LOCUS       DAZFFG010000001       138592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFFG010000001 DAZFFG010000000
VERSION     DAZFFG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 138592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 138592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..138592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.001"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(213..785)
                     /gene="gmhB"
                     /locus_tag="ACHJ7J_000001"
     CDS             complement(213..785)
                     /gene="gmhB"
                     /locus_tag="ACHJ7J_000001"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO4998288.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
     gene            973..2004
                     /gene="metN"
                     /locus_tag="ACHJ7J_000002"
     CDS             973..2004
                     /gene="metN"
                     /locus_tag="ACHJ7J_000002"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO4998289.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            1997..2650
                     /gene="metI"
                     /locus_tag="ACHJ7J_000003"
     CDS             1997..2650
                     /gene="metI"
                     /locus_tag="ACHJ7J_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO4998290.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2690..3505
                     /gene="metQ"
                     /locus_tag="ACHJ7J_000004"
     CDS             2690..3505
                     /gene="metQ"
                     /locus_tag="ACHJ7J_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO4998291.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3623..4027
                     /gene="rcsF"
                     /locus_tag="ACHJ7J_000005"
     CDS             3623..4027
                     /gene="rcsF"
                     /locus_tag="ACHJ7J_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO4998292.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            4024..4731
                     /gene="tsaA"
                     /locus_tag="ACHJ7J_000006"
     CDS             4024..4731
                     /gene="tsaA"
                     /locus_tag="ACHJ7J_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO4998293.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDACASYAQSAPAAE
                     MTVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRITDAGFEVFALEPR"
     gene            4842..6560
                     /gene="proS"
                     /locus_tag="ACHJ7J_000007"
     CDS             4842..6560
                     /gene="proS"
                     /locus_tag="ACHJ7J_000007"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO4998294.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEDSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6613..7437
                     /gene="yaeF"
                     /locus_tag="ACHJ7J_000008"
     CDS             6613..7437
                     /gene="yaeF"
                     /locus_tag="ACHJ7J_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO4998295.1"
                     /translation="MDKPKAYYRLLLPCFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(7637..8347)
                     /gene="nlpE"
                     /locus_tag="ACHJ7J_000009"
                     /gene_synonym="cutF"
     CDS             complement(7637..8347)
                     /gene="nlpE"
                     /locus_tag="ACHJ7J_000009"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO4998296.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8361..8783)
                     /gene="arfB"
                     /locus_tag="ACHJ7J_000010"
     CDS             complement(8361..8783)
                     /gene="arfB"
                     /locus_tag="ACHJ7J_000010"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO4998297.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            complement(8780..9325)
                     /gene="yaeQ"
                     /locus_tag="ACHJ7J_000011"
     CDS             complement(8780..9325)
                     /gene="yaeQ"
                     /locus_tag="ACHJ7J_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO4998298.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            9491..9691
                     /gene="yaeP"
                     /locus_tag="ACHJ7J_000012"
     CDS             9491..9691
                     /gene="yaeP"
                     /locus_tag="ACHJ7J_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO4998299.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9678..9938
                     /gene="rof"
                     /locus_tag="ACHJ7J_000013"
     CDS             9678..9938
                     /gene="rof"
                     /locus_tag="ACHJ7J_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO4998300.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9987..11285)
                     /gene="tilS"
                     /locus_tag="ACHJ7J_000014"
     CDS             complement(9987..11285)
                     /gene="tilS"
                     /locus_tag="ACHJ7J_000014"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO4998301.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(11350..11739)
                     /gene="yaeR"
                     /locus_tag="ACHJ7J_000015"
     CDS             complement(11350..11739)
                     /gene="yaeR"
                     /locus_tag="ACHJ7J_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4998302.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11796..13937)
                     /gene="ldcC"
                     /locus_tag="ACHJ7J_000016"
     CDS             complement(11796..13937)
                     /gene="ldcC"
                     /locus_tag="ACHJ7J_000016"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO4998303.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVVATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(14036..14995)
                     /gene="accA"
                     /locus_tag="ACHJ7J_000017"
     CDS             complement(14036..14995)
                     /gene="accA"
                     /locus_tag="ACHJ7J_000017"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO4998304.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(15008..18490)
                     /gene="dnaE"
                     /locus_tag="ACHJ7J_000018"
     CDS             complement(15008..18490)
                     /gene="dnaE"
                     /locus_tag="ACHJ7J_000018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO4998305.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18527..19123)
                     /gene="rnhB"
                     /locus_tag="ACHJ7J_000019"
     CDS             complement(18527..19123)
                     /gene="rnhB"
                     /locus_tag="ACHJ7J_000019"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO4998306.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(19120..20268)
                     /gene="lpxB"
                     /locus_tag="ACHJ7J_000020"
     CDS             complement(19120..20268)
                     /gene="lpxB"
                     /locus_tag="ACHJ7J_000020"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO4998307.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(20268..21056)
                     /gene="lpxA"
                     /locus_tag="ACHJ7J_000021"
     CDS             complement(20268..21056)
                     /gene="lpxA"
                     /locus_tag="ACHJ7J_000021"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO4998308.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(21060..21515)
                     /gene="fabZ"
                     /locus_tag="ACHJ7J_000022"
     CDS             complement(21060..21515)
                     /gene="fabZ"
                     /locus_tag="ACHJ7J_000022"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO4998309.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21620..22645)
                     /gene="lpxD"
                     /locus_tag="ACHJ7J_000023"
     CDS             complement(21620..22645)
                     /gene="lpxD"
                     /locus_tag="ACHJ7J_000023"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO4998310.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(22649..23134)
                     /gene="skp"
                     /locus_tag="ACHJ7J_000024"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22649..23134)
                     /gene="skp"
                     /locus_tag="ACHJ7J_000024"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO4998311.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(23256..25688)
                     /gene="bamA"
                     /locus_tag="ACHJ7J_000025"
     CDS             complement(23256..25688)
                     /gene="bamA"
                     /locus_tag="ACHJ7J_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO4998312.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25718..27070)
                     /gene="rseP"
                     /locus_tag="ACHJ7J_000026"
     CDS             complement(25718..27070)
                     /gene="rseP"
                     /locus_tag="ACHJ7J_000026"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO4998313.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(27082..27939)
                     /gene="cdsA"
                     /locus_tag="ACHJ7J_000027"
     CDS             complement(27082..27939)
                     /gene="cdsA"
                     /locus_tag="ACHJ7J_000027"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4998314.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27952..28710)
                     /gene="ispU"
                     /locus_tag="ACHJ7J_000028"
                     /gene_synonym="uppS"
     CDS             complement(27952..28710)
                     /gene="ispU"
                     /locus_tag="ACHJ7J_000028"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO4998315.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28899..30095)
                     /gene="ispC"
                     /locus_tag="ACHJ7J_000029"
     CDS             complement(28899..30095)
                     /gene="ispC"
                     /locus_tag="ACHJ7J_000029"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO4998316.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREAARKE
                     VMRLAS"
     gene            complement(30189..30746)
                     /gene="frr"
                     /locus_tag="ACHJ7J_000030"
     CDS             complement(30189..30746)
                     /gene="frr"
                     /locus_tag="ACHJ7J_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO4998317.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(31038..31763)
                     /gene="pyrH"
                     /locus_tag="ACHJ7J_000031"
     CDS             complement(31038..31763)
                     /gene="pyrH"
                     /locus_tag="ACHJ7J_000031"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO4998318.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31910..32761)
                     /gene="tsf"
                     /locus_tag="ACHJ7J_000032"
     CDS             complement(31910..32761)
                     /gene="tsf"
                     /locus_tag="ACHJ7J_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO4998319.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32896..33621)
                     /gene="rpsB"
                     /locus_tag="ACHJ7J_000033"
     CDS             complement(32896..33621)
                     /gene="rpsB"
                     /locus_tag="ACHJ7J_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO4998320.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33989..34783
                     /gene="map"
                     /locus_tag="ACHJ7J_000034"
     CDS             33989..34783
                     /gene="map"
                     /locus_tag="ACHJ7J_000034"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO4998321.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34845..37517
                     /gene="glnD"
                     /locus_tag="ACHJ7J_000035"
     CDS             34845..37517
                     /gene="glnD"
                     /locus_tag="ACHJ7J_000035"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO4998322.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37548..38372
                     /gene="dapD"
                     /locus_tag="ACHJ7J_000036"
     CDS             37548..38372
                     /gene="dapD"
                     /locus_tag="ACHJ7J_000036"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO4998323.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38686..39072
                     /gene="yaeH"
                     /locus_tag="ACHJ7J_000037"
     CDS             38686..39072
                     /gene="yaeH"
                     /locus_tag="ACHJ7J_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO4998324.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(39161..40318)
                     /gene="cdaR"
                     /locus_tag="ACHJ7J_000038"
     CDS             complement(39161..40318)
                     /gene="cdaR"
                     /locus_tag="ACHJ7J_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO4998325.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(40473..41897)
                     /gene="degP"
                     /locus_tag="ACHJ7J_000039"
     CDS             complement(40473..41897)
                     /gene="degP"
                     /locus_tag="ACHJ7J_000039"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO4998326.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(42027..43544)
                     /gene="dgt"
                     /locus_tag="ACHJ7J_000040"
     CDS             complement(42027..43544)
                     /gene="dgt"
                     /locus_tag="ACHJ7J_000040"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO4998327.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            43628..44326
                     /gene="mtnN"
                     /locus_tag="ACHJ7J_000041"
     CDS             43628..44326
                     /gene="mtnN"
                     /locus_tag="ACHJ7J_000041"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO4998328.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            44319..45119
                     /gene="btuF"
                     /locus_tag="ACHJ7J_000042"
     CDS             44319..45119
                     /gene="btuF"
                     /locus_tag="ACHJ7J_000042"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO4998329.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            45157..45780
                     /gene="yadS"
                     /locus_tag="ACHJ7J_000043"
     CDS             45157..45780
                     /gene="yadS"
                     /locus_tag="ACHJ7J_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO4998330.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(45827..46171)
                     /gene="erpA"
                     /locus_tag="ACHJ7J_000044"
     CDS             complement(45827..46171)
                     /gene="erpA"
                     /locus_tag="ACHJ7J_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO4998331.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(46164..46229)
                     /gene="yadW"
                     /locus_tag="ACHJ7J_000045"
     CDS             complement(46164..46229)
                     /gene="yadW"
                     /locus_tag="ACHJ7J_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO4998332.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(46253..47674)
                     /gene="clcA"
                     /locus_tag="ACHJ7J_000046"
     CDS             complement(46253..47674)
                     /gene="clcA"
                     /locus_tag="ACHJ7J_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO4998333.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(47686..47733)
                     /gene="yadX"
                     /locus_tag="ACHJ7J_000047"
     CDS             complement(47686..47733)
                     /gene="yadX"
                     /locus_tag="ACHJ7J_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO4998334.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            47899..49179
                     /gene="hemL"
                     /locus_tag="ACHJ7J_000048"
     CDS             47899..49179
                     /gene="hemL"
                     /locus_tag="ACHJ7J_000048"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO4998335.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(49214..51196)
                     /gene="fhuB"
                     /locus_tag="ACHJ7J_000049"
     CDS             complement(49214..51196)
                     /gene="fhuB"
                     /locus_tag="ACHJ7J_000049"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO4998336.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(51193..52083)
                     /gene="fhuD"
                     /locus_tag="ACHJ7J_000050"
     CDS             complement(51193..52083)
                     /gene="fhuD"
                     /locus_tag="ACHJ7J_000050"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO4998337.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(52083..52880)
                     /gene="fhuC"
                     /locus_tag="ACHJ7J_000051"
     CDS             complement(52083..52880)
                     /gene="fhuC"
                     /locus_tag="ACHJ7J_000051"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO4998338.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTALIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLENWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(52931..55174)
                     /gene="fhuA"
                     /locus_tag="ACHJ7J_000052"
     CDS             complement(52931..55174)
                     /gene="fhuA"
                     /locus_tag="ACHJ7J_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO4998339.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAATTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            complement(55394..57928)
                     /gene="mrcB"
                     /locus_tag="ACHJ7J_000053"
     CDS             complement(55394..57928)
                     /gene="mrcB"
                     /locus_tag="ACHJ7J_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO4998340.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(58124..60553)
                     /gene="hrpB"
                     /locus_tag="ACHJ7J_000054"
     CDS             complement(58124..60553)
                     /gene="hrpB"
                     /locus_tag="ACHJ7J_000054"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO4998341.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVRRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            60627..61157
                     /gene="thpR"
                     /locus_tag="ACHJ7J_000055"
     CDS             60627..61157
                     /gene="thpR"
                     /locus_tag="ACHJ7J_000055"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO4998342.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            61172..61876
                     /gene="sfsA"
                     /locus_tag="ACHJ7J_000056"
     CDS             61172..61876
                     /gene="sfsA"
                     /locus_tag="ACHJ7J_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO4998343.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSKSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            62054..62509
                     /gene="dksA"
                     /locus_tag="ACHJ7J_000057"
     CDS             62054..62509
                     /gene="dksA"
                     /locus_tag="ACHJ7J_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO4998344.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            62576..63472
                     /gene="gluQRS"
                     /locus_tag="ACHJ7J_000058"
     CDS             62576..63472
                     /gene="gluQRS"
                     /locus_tag="ACHJ7J_000058"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO4998345.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPGNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            63532..64929
                     /gene="pcnB"
                     /locus_tag="ACHJ7J_000059"
     CDS             63532..64929
                     /gene="pcnB"
                     /locus_tag="ACHJ7J_000059"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO4998346.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            64926..65405
                     /gene="folK"
                     /locus_tag="ACHJ7J_000060"
     CDS             64926..65405
                     /gene="folK"
                     /locus_tag="ACHJ7J_000060"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO4998347.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKTLGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVPQPAIW"
     gene            65748..66344
                     /locus_tag="ACHJ7J_000061"
     CDS             65748..66344
                     /locus_tag="ACHJ7J_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4998348.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            66431..67156
                     /locus_tag="ACHJ7J_000062"
     CDS             66431..67156
                     /locus_tag="ACHJ7J_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4998349.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVAPFSQASFGVTGLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            67198..69798
                     /locus_tag="ACHJ7J_000063"
     CDS             67198..69798
                     /locus_tag="ACHJ7J_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO4998350.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSGNSSAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACITHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVRFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLKTTYEDYGSLFISGSWTDYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINAPGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELTVRWYENNQTIDCKLHYQFPAQPQTLGSTNTLLL
                     NNLTCQVANH"
     gene            69812..70360
                     /locus_tag="ACHJ7J_000064"
     CDS             69812..70360
                     /locus_tag="ACHJ7J_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4998351.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVQD
                     GGPVVTGSNGEYSLTIPDVGLDKVVTGDVAAQANFKLVASDCSNGYSKIFTTLTGTTV
                     SGKLIVNETTSGATGVGMGIKRRDTADSAFFTPNNTDKFEWSTDEKTNGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            70375..70986
                     /locus_tag="ACHJ7J_000065"
     CDS             70375..70986
                     /locus_tag="ACHJ7J_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4998352.1"
                     /translation="MKLKVIATLIATVAVGVSVSNNLTYASTTSASLTVNSNLTMGTC
                     SAKVVDTSGAVIDTVVLGNVYISELVAKSKVQPFKIRFSDCAGLPGKSAQLVLAPKNN
                     GCAGGMSDTAAFSNKLNTTTDGGSNRAAVEVWTTTSPEGTGSEQFNCYQRNVINVDLS
                     NASTTQPYDFDLSARMIIADGWTVNDVLPGRFLSPTTFTITYQ"
     gene            71007..71597
                     /locus_tag="ACHJ7J_000066"
     CDS             71007..71597
                     /locus_tag="ACHJ7J_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4998353.1"
                     /translation="MLCRHHKFVRFLGLATALIAPFAYAGQDVDLTAQIVASTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQVNNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            71609..72721
                     /locus_tag="ACHJ7J_000067"
     CDS             71609..72721
                     /locus_tag="ACHJ7J_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4998354.1"
                     /translation="MQRKGNKLLIQLCSAILLFFTTSWYALANECYIERNAEGPYDMV
                     INPKPFSIQSAMVTVPEEISFATWDSHIDLRGDKIGCKSLGSNTSSVHFLNTADASLL
                     STYTTNHGNALLKTTVPGIVYSVELVCTSCGAAEELDLYLPAQSGADNHTDNASSKWA
                     YEYSDDSWYLRFRFYWTPEFKPQNGVSEGTAMPGKIASWYIGNNDQPWINFFVDTSSV
                     HFYVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKI
                     TVKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAVWSN
                     NLLLLPFTAQLVPDGSGAAVGVGTFSGNATFSFTYE"
     gene            72857..73651
                     /gene="panB"
                     /locus_tag="ACHJ7J_000068"
     CDS             72857..73651
                     /gene="panB"
                     /locus_tag="ACHJ7J_000068"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO4998355.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALVIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            73663..74514
                     /gene="panC"
                     /locus_tag="ACHJ7J_000069"
     CDS             73663..74514
                     /gene="panC"
                     /locus_tag="ACHJ7J_000069"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO4998356.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLQEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(74610..74819)
                     /locus_tag="ACHJ7J_000070"
     CDS             complement(74610..74819)
                     /locus_tag="ACHJ7J_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998357.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYQEELQKVRQEFAQRFLSK
                     GMSREDVAEVTTLPLTEIDKLINSN"
     gene            complement(74888..75760)
                     /gene="rpnC"
                     /locus_tag="ACHJ7J_000071"
     CDS             complement(74888..75760)
                     /gene="rpnC"
                     /locus_tag="ACHJ7J_000071"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO4998358.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGETTPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEFVLRLLNKGMSREDVAE
                     MANLPLAEIDKLIN"
     gene            76034..76414
                     /gene="panD"
                     /locus_tag="ACHJ7J_000072"
     CDS             76034..76414
                     /gene="panD"
                     /locus_tag="ACHJ7J_000072"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO4998359.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(76418..77647)
                     /gene="yadE"
                     /locus_tag="ACHJ7J_000073"
     CDS             complement(76418..77647)
                     /gene="yadE"
                     /locus_tag="ACHJ7J_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4998360.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(77711..78151)
                     /gene="yadI"
                     /locus_tag="ACHJ7J_000074"
     CDS             complement(77711..78151)
                     /gene="yadI"
                     /locus_tag="ACHJ7J_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4998361.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(78256..79026)
                     /gene="yadH"
                     /locus_tag="ACHJ7J_000075"
     CDS             complement(78256..79026)
                     /gene="yadH"
                     /locus_tag="ACHJ7J_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4998362.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(79023..79949)
                     /gene="yadG"
                     /locus_tag="ACHJ7J_000076"
     CDS             complement(79023..79949)
                     /gene="yadG"
                     /locus_tag="ACHJ7J_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4998363.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            80058..80720
                     /gene="can"
                     /locus_tag="ACHJ7J_000077"
     CDS             80058..80720
                     /gene="can"
                     /locus_tag="ACHJ7J_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO4998364.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(80761..81297)
                     /gene="hpt"
                     /locus_tag="ACHJ7J_000078"
     CDS             complement(80761..81297)
                     /gene="hpt"
                     /locus_tag="ACHJ7J_000078"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO4998365.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            81503..83893
                     /gene="gcd"
                     /locus_tag="ACHJ7J_000079"
     CDS             81503..83893
                     /gene="gcd"
                     /locus_tag="ACHJ7J_000079"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO4998366.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRYSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            83932..84030
                     /locus_tag="ACHJ7J_000080"
                     /pseudo
     CDS             83932..84030
                     /locus_tag="ACHJ7J_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(84095..85645)
                     /gene="cueO"
                     /locus_tag="ACHJ7J_000081"
     CDS             complement(84095..85645)
                     /gene="cueO"
                     /locus_tag="ACHJ7J_000081"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO4998367.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            85811..86158
                     /gene="yacC"
                     /locus_tag="ACHJ7J_000082"
     CDS             85811..86158
                     /gene="yacC"
                     /locus_tag="ACHJ7J_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO4998368.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            86264..87130
                     /gene="speE"
                     /locus_tag="ACHJ7J_000083"
     CDS             86264..87130
                     /gene="speE"
                     /locus_tag="ACHJ7J_000083"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO4998369.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            87146..87940
                     /gene="speD"
                     /locus_tag="ACHJ7J_000084"
     CDS             87146..87940
                     /gene="speD"
                     /locus_tag="ACHJ7J_000084"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO4998370.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(87978..88340)
                     /gene="yacL"
                     /locus_tag="ACHJ7J_000085"
     CDS             complement(87978..88340)
                     /gene="yacL"
                     /locus_tag="ACHJ7J_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO4998371.1"
                     /translation="MDYEFLRDTTGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(88515..91112)
                     /gene="acnB"
                     /locus_tag="ACHJ7J_000086"
     CDS             complement(88515..91112)
                     /gene="acnB"
                     /locus_tag="ACHJ7J_000086"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO4998372.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVCNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            91467..93314
                     /gene="yacH"
                     /locus_tag="ACHJ7J_000087"
     CDS             91467..93314
                     /gene="yacH"
                     /locus_tag="ACHJ7J_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO4998373.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAILAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHDDDYHHHDGGHRDGNGWQHNGD
                     NINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQ
                     LPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFSRR
                     QPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQAV
                     REKMQTNPQIQQRREAARERIQSASPEQRQVFKEKVQQSPLDQQQRDNARQRVQSASP
                     EQRQVFREKVKESHPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(93385..94809)
                     /gene="lpdA"
                     /locus_tag="ACHJ7J_000088"
     CDS             complement(93385..94809)
                     /gene="lpdA"
                     /locus_tag="ACHJ7J_000088"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO4998374.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(95017..96909)
                     /gene="aceF"
                     /locus_tag="ACHJ7J_000089"
     CDS             complement(95017..96909)
                     /gene="aceF"
                     /locus_tag="ACHJ7J_000089"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO4998375.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(96924..99587)
                     /gene="aceE"
                     /locus_tag="ACHJ7J_000090"
     CDS             complement(96924..99587)
                     /gene="aceE"
                     /locus_tag="ACHJ7J_000090"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO4998376.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(99748..100512)
                     /gene="pdhR"
                     /locus_tag="ACHJ7J_000091"
     CDS             complement(99748..100512)
                     /gene="pdhR"
                     /locus_tag="ACHJ7J_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO4998377.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            101053..102426
                     /gene="aroP"
                     /locus_tag="ACHJ7J_000092"
     CDS             101053..102426
                     /gene="aroP"
                     /locus_tag="ACHJ7J_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO4998378.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKLEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(102469..103323)
                     /gene="ampE"
                     /locus_tag="ACHJ7J_000093"
     CDS             complement(102469..103323)
                     /gene="ampE"
                     /locus_tag="ACHJ7J_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO4998379.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(103320..103871)
                     /gene="ampD"
                     /locus_tag="ACHJ7J_000094"
     CDS             complement(103320..103871)
                     /gene="ampD"
                     /locus_tag="ACHJ7J_000094"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO4998380.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTNAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            103959..104852
                     /gene="nadC"
                     /locus_tag="ACHJ7J_000095"
     CDS             103959..104852
                     /gene="nadC"
                     /locus_tag="ACHJ7J_000095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO4998381.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            105055..105495
                     /gene="ppdD"
                     /locus_tag="ACHJ7J_000096"
     CDS             105055..105495
                     /gene="ppdD"
                     /locus_tag="ACHJ7J_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO4998382.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN"
     gene            105505..106890
                     /gene="gspE"
                     /locus_tag="ACHJ7J_000097"
     CDS             105505..106890
                     /gene="gspE"
                     /locus_tag="ACHJ7J_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO4998383.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGELIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            106880..108082
                     /gene="hofC"
                     /locus_tag="ACHJ7J_000098"
     CDS             106880..108082
                     /gene="hofC"
                     /locus_tag="ACHJ7J_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO4998384.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMRWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(108117..109160)
                     /gene="guaC"
                     /locus_tag="ACHJ7J_000099"
     CDS             complement(108117..109160)
                     /gene="guaC"
                     /locus_tag="ACHJ7J_000099"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO4998385.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(109316..109360)
                     /locus_tag="ACHJ7J_000100"
     CDS             complement(109316..109360)
                     /locus_tag="ACHJ7J_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998386.1"
                     /translation="MIIKTLKMSARKRN"
     gene            109385..110005
                     /gene="coaE"
                     /locus_tag="ACHJ7J_000101"
     CDS             109385..110005
                     /gene="coaE"
                     /locus_tag="ACHJ7J_000101"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO4998387.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            110005..110748
                     /gene="zapD"
                     /locus_tag="ACHJ7J_000102"
     CDS             110005..110748
                     /gene="zapD"
                     /locus_tag="ACHJ7J_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO4998388.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            110758..110955
                     /gene="yacG"
                     /locus_tag="ACHJ7J_000103"
     CDS             110758..110955
                     /gene="yacG"
                     /locus_tag="ACHJ7J_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO4998389.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(111286..111675)
                     /gene="mutT"
                     /locus_tag="ACHJ7J_000104"
     CDS             complement(111286..111675)
                     /gene="mutT"
                     /locus_tag="ACHJ7J_000104"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO4998390.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(111735..114440)
                     /gene="secA"
                     /locus_tag="ACHJ7J_000105"
     CDS             complement(111735..114440)
                     /gene="secA"
                     /locus_tag="ACHJ7J_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO4998391.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(114502..115014)
                     /gene="secM"
                     /locus_tag="ACHJ7J_000106"
     CDS             complement(114502..115014)
                     /gene="secM"
                     /locus_tag="ACHJ7J_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO4998392.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(115245..116162)
                     /gene="lpxC"
                     /locus_tag="ACHJ7J_000107"
     CDS             complement(115245..116162)
                     /gene="lpxC"
                     /locus_tag="ACHJ7J_000107"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO4998393.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(116263..117414)
                     /gene="ftsZ"
                     /locus_tag="ACHJ7J_000108"
     CDS             complement(116263..117414)
                     /gene="ftsZ"
                     /locus_tag="ACHJ7J_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO4998394.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(117475..118737)
                     /gene="ftsA"
                     /locus_tag="ACHJ7J_000109"
     CDS             complement(117475..118737)
                     /gene="ftsA"
                     /locus_tag="ACHJ7J_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO4998395.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(118734..119564)
                     /gene="ftsQ"
                     /locus_tag="ACHJ7J_000110"
     CDS             complement(118734..119564)
                     /gene="ftsQ"
                     /locus_tag="ACHJ7J_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO4998396.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(119566..120486)
                     /gene="ddlB"
                     /locus_tag="ACHJ7J_000111"
     CDS             complement(119566..120486)
                     /gene="ddlB"
                     /locus_tag="ACHJ7J_000111"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4998397.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(120479..121954)
                     /gene="murC"
                     /locus_tag="ACHJ7J_000112"
     CDS             complement(120479..121954)
                     /gene="murC"
                     /locus_tag="ACHJ7J_000112"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO4998398.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(122008..123075)
                     /gene="murG"
                     /locus_tag="ACHJ7J_000113"
     CDS             complement(122008..123075)
                     /gene="murG"
                     /locus_tag="ACHJ7J_000113"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO4998399.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(123072..124316)
                     /gene="ftsW"
                     /locus_tag="ACHJ7J_000114"
     CDS             complement(123072..124316)
                     /gene="ftsW"
                     /locus_tag="ACHJ7J_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO4998400.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(124316..125632)
                     /gene="murD"
                     /locus_tag="ACHJ7J_000115"
     CDS             complement(124316..125632)
                     /gene="murD"
                     /locus_tag="ACHJ7J_000115"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO4998401.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKISGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(125635..126717)
                     /gene="mraY"
                     /locus_tag="ACHJ7J_000116"
     CDS             complement(125635..126717)
                     /gene="mraY"
                     /locus_tag="ACHJ7J_000116"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO4998402.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(126711..128069)
                     /gene="murF"
                     /locus_tag="ACHJ7J_000117"
     CDS             complement(126711..128069)
                     /gene="murF"
                     /locus_tag="ACHJ7J_000117"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO4998403.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(128066..129553)
                     /gene="murE"
                     /locus_tag="ACHJ7J_000118"
     CDS             complement(128066..129553)
                     /gene="murE"
                     /locus_tag="ACHJ7J_000118"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO4998404.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEEHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(129540..131306)
                     /gene="ftsI"
                     /locus_tag="ACHJ7J_000119"
     CDS             complement(129540..131306)
                     /gene="ftsI"
                     /locus_tag="ACHJ7J_000119"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO4998405.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(131322..131687)
                     /gene="ftsL"
                     /locus_tag="ACHJ7J_000120"
     CDS             complement(131322..131687)
                     /gene="ftsL"
                     /locus_tag="ACHJ7J_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO4998406.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(131684..132625)
                     /gene="rsmH"
                     /locus_tag="ACHJ7J_000121"
     CDS             complement(131684..132625)
                     /gene="rsmH"
                     /locus_tag="ACHJ7J_000121"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO4998407.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMIRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(132627..133085)
                     /gene="mraZ"
                     /locus_tag="ACHJ7J_000122"
     CDS             complement(132627..133085)
                     /gene="mraZ"
                     /locus_tag="ACHJ7J_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO4998408.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(133687..134691)
                     /gene="cra"
                     /locus_tag="ACHJ7J_000123"
     CDS             complement(133687..134691)
                     /gene="cra"
                     /locus_tag="ACHJ7J_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO4998409.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(134773..134859)
                     /locus_tag="ACHJ7J_000124"
     CDS             complement(134773..134859)
                     /locus_tag="ACHJ7J_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO4998410.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(134871..135362)
                     /gene="ilvN"
                     /locus_tag="ACHJ7J_000125"
     CDS             complement(134871..135362)
                     /gene="ilvN"
                     /locus_tag="ACHJ7J_000125"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4998411.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(135365..137089)
                     /gene="ilvI"
                     /locus_tag="ACHJ7J_000126"
     CDS             complement(135365..137089)
                     /gene="ilvI"
                     /locus_tag="ACHJ7J_000126"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO4998412.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(137407..138351)
                     /gene="leuO"
                     /locus_tag="ACHJ7J_000127"
     CDS             complement(137407..138351)
                     /gene="leuO"
                     /locus_tag="ACHJ7J_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO4998413.1"
                     /translation="MPEVQTDHPEMAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        33531 a        37907 c        34137 g        33017 t
ORIGIN      
        1 ggctttcagc tcactgtcgc gaggtggcgt atattacgct ttcctctttc agagtcaacc
       61 ctgaatttca ggattttttc tcttcaaccg aaccggctgt ttgtgtgaag tgattcacat
      121 ccgccgtgtc gatggaggcg cattataggg agtcgtttca ggaagacaag cggaaaaatg
      181 cttttttatt tcaaccgctc atcttttaat cattacgccg gtttttgctg cttttttatc
      241 gcttgcggaa ggtctgccag gctatttaac acccaatccg ccgcgttttc tgcttcaggc
      301 gtaacaggtt tacccgtacg caccagcact tttgttccca cgttcgccgc agccgctgcc
      361 tgcatatctt ctaatttatc gcccaccata taagaagcgg ccatatcaat atgcaaataa
      421 tcgcgtgctg acaaaagcat ccccggatgt ggtttgcggc agtcgcaaac ctggcgaaac
      481 tcttcaacac taccctgcgg atgatgcggg caataataga tgccatccag atcgacatcg
      541 cggtccgcca gcgaccagtc catccactcg gtcagcgttt caaactgcgc ctcagtaaat
      601 ttaccgcgtg caataccaga ctggttagtc accaccacca gcgcaaagcc catttttttt
      661 agctcgcgca tggcgtcaat aacaccgtcg ataaattcaa agttgtcgat ctcatggaca
      721 tagccgtgat cgacattaat ggtgccatca cggtcaagaa aaattgcggg tacgctcttc
      781 gccacctttt atagctcctt aataaggcat gtgacgctag tatcgcatgt ttcgacctgc
      841 aagaaagtgc tcttcgcata aacctgattg atttagacgt ctggatgcct taacatccat
      901 ttcattgacg gcgttgcccg tttcaggcat gcgaaatgcc acgactaact taatgacgat
      961 aataaataat caatgataaa actttcgaat atcaccaaag tgttccacca gggcacccgc
     1021 accatccagg cgttgaacaa cgtcagcctg catgtgccag ctggacaaat ttatggcgtt
     1081 atcggtgcct caggcgcggg taagagtacg cttatacgtt gtgtaaacct gctggagcgc
     1141 ccaaccgagg gtagcgtgct ggtcgatggc caggaactga ccacgctgtc agaatccgag
     1201 ttgaccaaag ctcgtcgcca gattggtatg attttccagc attttaacct gctctcttcg
     1261 cgtactgttt ttggcaacgt ggctctgccg ctggagctgg acaatacacc gaaagacgag
     1321 atcaaacgtc gcgtgacgga attgctgtca ttggttggtc ttggagataa gcatgatagc
     1381 tacccgtcga atctttccgg tgggcagaaa caacgtgtgg cgattgcccg tgcattagcc
     1441 agcaatccca aagtattgct gtgtgatgaa gccaccagcg cgctagaccc agcaacgaca
     1501 cgttctattc tcgaactgct gaaagacatt aaccgccgtc tgggtttgac gattctgttg
     1561 atcacccacg aaatggacgt tgtgaagcgc atttgtgatt gcgtggcggt tatcagtaat
     1621 ggcgaactga tcgagcagga cacggtaagt gaagtgttct cgcatccgaa aacgccgctg
     1681 gcgcagaagt ttattcagtc gaccctgcat ctggatatcc cggaagatta ccaggaacgt
     1741 ctgcaagcgg agccatttac tgactgcgtg ccgatgctgc gtctggagtt taccggtcaa
     1801 tcggtcgatg ccccactgct ttctgaaacc gcgcgtcgtt tcaacgtcaa caacaacatt
     1861 attagcgcgc agatggatta cgccggtggc gtgaagttcg gcatcatgct gactgaaatg
     1921 cacggcacac aacaagatac gcaagccgcc attgcctggc tgcaggaaca ccatgtaaaa
     1981 gtagaggtac tgggttatgt ctgagccgat gatgtggctg ctggttcgtg gcgtatggga
     2041 aacgctggca atgaccttcg tatccggatt ttttgggttt gtgattggtc tgccggttgg
     2101 cgtcctgctt tatgtcacgc gtccggggca aattattgct aacgcgaagc tgtatcgtac
     2161 cgtttctgcg attgtgaaca ttttccgttc catcccgttc attatcttgc tggtatggat
     2221 gattccgttt acccgcgtta ttgtcggtac atcgattggt ttgcaggcag caattgttcc
     2281 gttaaccgtt ggtgcagcac cgtttattgc ccgtatggtc gagaacgctc tgctggagat
     2341 cccaaccggg ttaattgaag cttcccgcgc aatgggtgcc acgccaatgc agatcgtccg
     2401 taaagtgctg ttaccggaag cgttgccggg tctggtgaat gcggcaacta tcaccctgat
     2461 taccctggtc ggttattccg cgatgggtgg tgcagtcggt gccggtggtt taggtcagat
     2521 tggctatcag tatggctaca tcggctacaa cgcgacggtg atgaatacgg tactggtatt
     2581 gctggtcatt ctggtttatt taattcagtt cgcaggcgac cgcatcgtcc gggctgtcac
     2641 tcgcaagtaa cgttcaacac aacataaata attgaagaag gaataaggta tggcgttcaa
     2701 attcaaaacc tttgcggcag tgggagccct gattggatca ctggcactgg taggctgcgg
     2761 tcaggatgaa aaagatccaa accacattaa agtcggcgtg attgttggtg ccgaacagca
     2821 ggttgcagaa gtcgcgcaga aagttgcgaa agacaaatat ggcctggacg ttgagctggt
     2881 aaccttcaac gactatgttc tgccaaacga agcattgagc aaaggtgata tcgacgccaa
     2941 cgccttccag cataaaccgt accttgatca gcaactgaaa gatcgtggct acaagctggt
     3001 cgcagtaggc aatacttttg tttatccaat tgctggttac tccaagaaaa tcaaatcact
     3061 ggatgaactg caggatggtt cgcaggttgc cgtgccaaac gacccaacta accttggtcg
     3121 ttcactgctg ctgctgcaaa aagtgggctt gatcaaactg aaagatggcg ttggccttct
     3181 gccgaccgtt cttgatgttg ttgagaaccc aaaaaatctg aaaattgttg agctggaagc
     3241 accgcaactg ccgcgttctc tggacgacgc gcaaatcgct ctggcagtta tcaataccac
     3301 ctatgccagc cagattggcc tgactccggc gaaagacggt atctttgtcg aagataaaga
     3361 ctccccgtac gtaaacctga tcgtaacgcg tgaagataac aaagacgccg agaacgtgaa
     3421 gaaattcgtc caggcttatc agtctgacga agtttacgaa gcagcaaaca aagtgtttaa
     3481 cggcggcgct gttaaaggct ggtaattttt aggctgtttc cacaatttgt aatatcattc
     3541 aggacgggcg cttgcccgtc ttgtcatttt tacaagctcc tgattcaata ttgacgtttt
     3601 gatcatacat tgaggaaata ctatgcgtgc tttaccgatc tgtttagtag cactcatgct
     3661 aagcggctgt tccatgttaa gcagatcccc tgtcgaaccc gttcaaagca ctgcacccca
     3721 gccgaaagcg gagcctgcaa aaccgaaagc gccgcgcgcc acgccggtcc gaatttatac
     3781 caatgcagaa gaattagtcg gcaaaccgtt ccgcgatctc ggtgaagtca gtggcgactc
     3841 ttgccaggcc tctaatcagg actctccgcc gagcattcca accgcacgta agcggatgca
     3901 aatcaacgcc tctaaaatga aagccaatgc ggtattactg catagctgcg aagtcaccag
     3961 cggtacgccg ggctgctatc gtcaggctgt atgtatcggt tctgcgctta acattacggc
     4021 gaaatgagca gtttccagtt cgagcaaata ggcgttattc gctcgcccta taaagaaaaa
     4081 ttcgccgttc cgcgccagcc aggtctggta aaaagcgcca acggcgaact gcatctcatt
     4141 gctccctaca accaggccga cgccgttcgc ggcctggaag cgttcagcca tttatggatc
     4201 cttttcgtct ttcatcaaac gatggaaggc ggctggcgtc cgactgtgcg tccaccgcgc
     4261 ctcggcggta acgccagaat gggggttttc gcaacacgct ctactttccg ccctaaccca
     4321 attggcatgt cgctggtaga gctgaaagag gttgtttgcc ataaagacag cgtgattctg
     4381 aagctcggca gtctggatct ggtcgatggt acgccggtag tggatatcaa accgtatctc
     4441 ccctttgccg aatcgcttcc cgatgcctgt gccagctatg cgcaaagtgc gccagcggca
     4501 gagatgacgg tgagttttac cgcagaggtc gaaaagcagc ttttgacgct ggagaagcgt
     4561 tatccgcagt taaccctgtt tatccgcgaa gtactggcgc aagacccgcg cccggcctat
     4621 cgtaaaggtg aggaaacggg caaaacctat gccgtctggc tgcatgattt taacgttcgc
     4681 tggcgcatta ccgacgcagg ttttgaagtc tttgcgctgg aaccgcgtta aattgacgcc
     4741 cttctctttt gacatttctt ttgcactggt aaactaaatc actttttttg tcccaggctc
     4801 gccttgagcc tgttctacct tccaactgga accgtaacaa catgcgtact agccaatacc
     4861 tgctctccac tctcaaggag acacctgccg acgccgaggt gatcagccat cagctgatgc
     4921 tgcgcgccgg gatgatccgc aagctggcct ccgggttata tacctggctg ccgaccggcg
     4981 tgcgcgttct gaaaaaagtc gaaaacatcg tgcgtgaaga gatgaacaac gccggtgcga
     5041 tcgaggtgtc gatgccggtg gttcagccag ccgatttgtg gcaagagagt ggtcgttggg
     5101 aacagtacgg tccggaactg ctgcgttttg ttgaccgtgg cgagcgtccg ttcgtactcg
     5161 gcccaactca tgaagaagtt atcactgacc tgattcgtaa cgagctgagc tcttacaaac
     5221 agctgccgct gaacttctat cagatccaga ccaagttccg cgatgaagtg cgtccgcgtt
     5281 tcggcgtcat gcgttcccgc gaattcctga tgaaagatgc ttactctttc catacttctc
     5341 aggaatccct gcaggaaacc tacgacgcaa tgtatgcggc ctacagcaaa atcttcagcc
     5401 gcatggggct ggatttccgc gccgtacagg ccgacaccgg ttctatcggc ggtagcgcct
     5461 ctcacgaatt ccaggtgctg gcgcagagcg gtgaagacga tgtggtcttc tccgacacct
     5521 ctgactatgc agcgaacatt gaactggcag aagctatcgc gccgaaagaa ccgcgcgctg
     5581 ctgctaccca ggaaatgacg ctggttgata cgccgaacgc gaagaccatc gcggaactgg
     5641 ttgaacagtt caatctgccg attgagaaaa ccgttaagac tctgctggtt aaagcggttg
     5701 aagacagtag cttcccgctg gttgctctgc tggtgcgcgg tgaccacgaa ctgaacgaag
     5761 ttaaagcaga aaaactgccg caggttgcca gcccgctgac ttttgcgact gaagaagaaa
     5821 ttcgtgccgt ggttaaagcc ggtccgggtt cactgggtcc ggtaaacatg ccgattccgg
     5881 tggtgattga ccgtaccgtt gcggcgatga gtgatttcgc tgctggtgct aacatcgatg
     5941 gtaaacacta cttcggcatc aactgggatc gcgatgtcgc taccccggaa gttgcggata
     6001 tccgcaacgt ggtggctggc gatccaagcc cggatggtca gggtacgctg ctgatcaaac
     6061 gtggtatcga agtcggtcac atcttccagc tgggtaccaa gtactccgaa gcactgaaag
     6121 cctccgtaca aggtgaagat ggccgtaacc agatcctgac tatgggttgc tacggtatcg
     6181 gcgtaacgcg cgtagtagct gcagccattg agcagaacta cgacgaacgc ggcatcgtat
     6241 ggcctgacgc gatcgcaccg ttccaggtgg cgattctgcc gatgaacatg cacaaatcct
     6301 tccgcgtaca agagcttgct gagaaactgt acagcgaact gcgtgcacaa ggtatcgaag
     6361 tgctgctgga tgaccgaaaa gaacgtccgg gcgtgatgtt tgccgatatg gaactgatcg
     6421 gtattccgca cactattgtg ctgggcgacc gtaacctcga caacgacgat atcgaatata
     6481 aatatcgtcg caacggcgag aaacagttaa ttaagactgg tgacatcgtc gaatatctgg
     6541 tgaaacagat taaaggctga tgccagaaag ggtcctgaat ttcagggccc ttttttacat
     6601 ggattgtata aaatggataa accaaaggcg tactaccgcc tgcttctccc ctgctttctt
     6661 ttactttcgg cctgtaccgt tgatatcagc cagccagact cctctgccac agcggtagat
     6721 gttgaggcaa aaacatgggc tgtcaaattc cagcatcaaa gctctttcac cgaacaatca
     6781 ataaaagaaa taacggtacc tgatctcaaa cccggcgatt tgctgttctc ctcaagcctg
     6841 ggggtaacct catttgggat ccgcgttttc agcacctcct ctgtgagtca cgttgcaatc
     6901 tatctgggtg aaaataacgt tgcagaagcg acaggcgctg gcgtccagat tgtttccctt
     6961 aaaaaagcga tgaagcatag cgataagctt ttcgtcttac gagtcccgga tcttaccccg
     7021 caacaagcca cagaaatcac cgcgttcgcc aacaaaatta aagatagcgg gtataactat
     7081 cgcggcattg tcgagttcat tccatttatg gtgacccgcc agatgtgctc actgaatcct
     7141 ttttccgcag attttcgcca acagtgcgtc agcggcctgg cgaaagcgca gttaagcagt
     7201 gtgggcgaag gagacaaaaa gtcgtggttt tgttcggaat ttgtcactga tgcatttgcc
     7261 aaagccgggc atccgcttac gctggcgcaa tcgggctgga ttagccctgc cgatctgatg
     7321 catatgcgta ctggcgatgt ctctgcgttt aagcccgaaa cgcagttaca gtatgttggg
     7381 cacctgaagc cagggattta tatcaaggcg ggtcgctttg ttggtttgac ccggtgataa
     7441 agtaaatgcc ttatccgcct taaggtatga ggggggttgt aggcatgata agacgcgcca
     7501 gcgtcgcatc aggcgttgag caccattgcc ggatgcggcg taaacgcctt atccggccta
     7561 caaaaaacgt gcaaattcaa aatgttgaaa tatatccttc tggcctgttt tgcgtttgtt
     7621 tctgtctcaa gacgggttac tgccccaaac tactgcaatc cttgttcggg taaaatttac
     7681 ccgccgtatc gggcgctaat actttggtcg gcgcaccggt atccggatta gcctccagcg
     7741 taaagtgacc ttcaactgac agtaacaccg gtttttcact gttaccccgc gcagccaggt
     7801 agccacgttc cagctctgcg ttattcgcta ccatgaaacg ttttccggtc gcgcaatcag
     7861 tgaaggtcgc cgcatcagcc atataaaaat acatgccccg cagggtcatc ggcgtcatgg
     7921 gtaaactgga ttgtgccggt tccagcgtat agttgaactg cgattcaatc ggattgcctt
     7981 cacgatcgag catctccagc gcatcgcctt tcgcccgata atatgacttt tcacctttgc
     8041 tgtcggttaa taccagcttg tcagcggttc gcgcccatgt accgtaggaa gcgaaggagg
     8101 aaggttcttc acgagcaccg agataacgct cattcatcac ccatgttccg tctttttcga
     8161 ggaacagaga ggtttcgatt ccttcgcaat cggcacacgg cagcacgccg cgccaacttt
     8221 gcggcatcgg tttcagttcg gctgcctgcg ccggagaaag cgtatcgact tcggcccgat
     8281 tattacaacc catcagagta aagaggctga ttacagccat cgctgtcact atcgcttttt
     8341 tcaccatcca ttccttcttt ttattcccga ccgctgcgca ctttgccgcg catcgccttc
     8401 acgcttgatt tttgtgcttt cgatgccagc ctgcgctctt tcgatgcacg ggtgggccgc
     8461 gtgggtcgtc gggctttttg ttctgttgtt aaatctttaa tcactgccac cagccgggcc
     8521 agcgcggctt cgcggttcag ttcctgactg cggtattcct gtgccttaat gacaatcacg
     8581 ccatcactgc tgatcaaatg atggctggcg gcgagcagac gctctttgta atactctggc
     8641 aggctggacg cccgaatgtc aaaacgcaga tgaatagccg ttgaggtctt attaacatgc
     8701 tgcccgcccg cgccctgcgc acgaatggcg gtgatctcaa gctcaccatc gggaatagca
     8761 acatgtcggg aaatcacaat catgaaggtt gttgccaggc ggttaagttc acttccagat
     8821 tattcttatc atccgataac cagatcacgc catcctgaat cgttgcctgc agcgtcatgg
     8881 tacgatcggc aaaggcgctt actttcgcca gttgttcatc gtccagatac cagacggaaa
     8941 gattggcaaa ctgcacacat ttgctctgat tttgctgcca ccagatttgc gccgcccgac
     9001 tattataggt aaacagcgcc acttctgcgg cctgggtgca ggctttctta atccgccgct
     9061 catccggcag ccccagctca atccacaaat caatgcccag gtgatcgtta cgcagccacg
     9121 cttccggctc atcatcggca cacaaaccac gggtaaattg cagacgttca tcagcatatt
     9181 tcaaccacgc cagcaagcgc agcatcatac gctcctgggt ttctgaaggg tggcgcgcca
     9241 gcgtcagaga ggcatcgaga aactggttgc ggtcgagatc ggccacatta accgtcgctt
     9301 tataaattgt cgctttaagc gccataagaa aactcctttc gaatcaggag gccattgtaa
     9361 cggaatacgt gcagttgcgg cgctgatatt gcagaaatga gtgtggtata gtcaccttgc
     9421 gaaacagagt ttatcttcgt aggcttagac ttgcatccac ggttaagtca gagtgctgac
     9481 aggagggcat gtggaaaaat attgtgagtt aatacgcaag cggtacgcgg aaatcgccag
     9541 cggagactta ggatacgttc cggacgcgct gggctgcgtt ttgaaagtgc tgaatgaaat
     9601 ggctgccgac gacgcccttt ctgaggctgt cagggaaaaa gcagcatacg ctgcggcaaa
     9661 cttactggtg agcgattatg tcaatgaatg atacgtatca accaatcaat tgtgatgatt
     9721 acgataatct tgagctcgcc tgccagcatc atttaatgct gacacttgag ctgaaagatg
     9781 gcgaaaaatt gcaggccaaa gccagcgatt tagtctcccg caaaaatgtg gagtatctgg
     9841 tcgtcgaggc cgctggcgaa acccgtgagc tacgtctgga taaaattacc agctttagcc
     9901 acccggaaat cggtacggtg gtggtaagcg aatcctgatt gacttttgcc ggatgcgacg
     9961 tttgatgcgt cttatccggc tttcacttaa ctaagcgttt tctgccagac aaaacttacg
    10021 cccttctcac cttcagccac accttcttgc gtcacaaata cccctgccgc cgcaatcagc
    10081 gtttcgccat aaaacagcag tggcgtggtg tcacgtagcc acggcggcac gcccagctcc
    10141 tgccagattt tcttcagctt acgtccgccg ttgcgaccga caatatgcag caatcctggc
    10201 gctttgaaac gcacgctgac cgcttcgtct gcacgcggag ggcgaatatc gcctcccgca
    10261 gtaagctgta cacttcccag ccccgccggt aattccagcg gttgtagcca cgtctgccac
    10321 ggcacaatgg tttcgctttg cccggcaacg gatttaatcc accacagttg cgattgatag
    10381 cggcgtattt caaacgcgcc aaagcgtaaa cagggggagg catcttcccg cgccagcgcc
    10441 acttcctgcc agatccgcac cagcgcgtcg cgggaaggca tcggtgcatt ctgccctgcc
    10501 agccagcggc ggataattgc cgcgcgacgg gcatcactca tcgccagcat tggcgcaatc
    10561 tgcagcgtcc cctgcggcgt ttggcagtgc gctaaatcat ctgccagcaa ttcatccagc
    10621 aggctctctt gttcagcaca aagtgcggcg ctgcgagccg ttgcttcggc aaaatgcggc
    10681 caacgctgct gcaataacgg cactacacgc aggcgcagaa agttacgatc gtatgagtcg
    10741 tcctgattac tttcgtcttc aatccagcgt aaaccatgcg ctaatgccca ctgcgccagt
    10801 tccccccgcg tgcgggcgag caacgggcgg ataagccgtg ttccggcaaa ctccgaaact
    10861 tccgccatag ccgaaagccc ggcagggccg ctaccacgtt ttagcgccag cagaaaggtt
    10921 tcacattgat cgtcgagatg ttgcgcggtg accagcactt caccgggcaa caaggtgcgg
    10981 gcaaatgcct gataacgcgc ctgccgcgcc tgggcctcaa tgcccagtcc ttcttgcgca
    11041 agttgcacgc gctcgaccac cagcggcacc tgccactgtt ggcagatgtt ctcgcaatgc
    11101 ctaacccagg catcggcatt ggcacttaaa ccgtgatgca catggatagc gcgtagagtg
    11161 acgcccggat tttccgtccg ccactgcacc aactgatgca gcagaacggt ggagtcaagc
    11221 ccgccgctaa aggccaccag aatctggcgt gaggtgagaa gttgtctatt gagcgtgagt
    11281 gtcatgatgg tgcgatttta cagtagcaat gcccggcacg ttaccgggca aatgccgcga
    11341 tgacaagcct tactgctcat acagttccaa cggcagcccg tccggatcgt taaagaaggt
    11401 gaagcgtttt tgcgtgtatg gatcgacacg gatggcttca cacttcacgt tatggctttc
    11461 aaggtgcgcc actgccgcat cgatgtcatc aacgctaaaa gccagatgac gcaggccgca
    11521 agcttccggt cggctggggc gctccggcgg gaacggaaat gagaaaagct caatcacata
    11581 ttgcccatta agcgccaaat cccctttcca cgagtcgcgc gcttcgcgat agacttcgct
    11641 ttgcagcgtg aaaccaagaa tatcgcagta gaaagctttg ctcaccgcat aatccgtcgc
    11701 aataatcgca atatggtgaa cctgttttaa acccagcata acgtctcctt tatttgttaa
    11761 cagcacgtta ctcgcccgga agccgctctg gcaagttatc ccgccatttt taggactcgt
    11821 acgcggtaaa cgccgtcttc gtcctgtttc gcaccgtgaa tatccgtttc aaaaccgggg
    11881 taatgttgcc ctacggaaca aagcatcagt agaaaatcga gtactgtgcg gctctctttg
    11941 gtcagcattt ctccaggcat caacagcggt acgcccggtg gataaggcag gatcatattt
    12001 gccgatactc taccgaccag ttgttccagc gcaatggttt ctacttcacc tttaatctgt
    12061 ctctgccatg cctgatgtgg cgtcatgatc atctccggca aagtatcgaa tgcccgtaac
    12121 atcaaaccgg gaagatcgtg tttacgaatc agcttatgga tcccttgcgc cagatcctga
    12181 atacgcatat tgcggtagaa atcgggatct tcagcataga gatcgggcag catatttttg
    12241 atccgcaggt tgagatcgta agagcgctta aattccgtca acccacgcaa taatcccatt
    12301 gctttggttt tatcgatgcc aatactaaag agaaacagca ggttataagg gccggttttc
    12361 tctactacga tcccacgttc gtcgaggaat tttgccacca gcgccgccgg gatcccctcc
    12421 tcactcatat tgccctgttc gtccatcccc ggtgtcaaaa tagtgacttt aaccggatcg
    12481 agaaacatat gatcggcatc cgcatcgtta aagccgtgcc actgttcgcc aggcgcaacg
    12541 ggccagcatt cggcttcatc cacctgcggc ggttgccaga tatcgaaaaa ccaaccgtca
    12601 gactcttccc gcaaccgctg gacctctttg cgaaaatgca gcgcccgctc caccgaacgg
    12661 ttaatcagcc gtttacccgg attaccacgc agcatcgctg ctgccgtctc aaccgaagca
    12721 acaatgggat aactgggcga ggtggtggta tgcatcataa aggcttcgtt aaaggcctct
    12781 tcgtcatact cgcctttaat gtggatcagc gaagcctgcg ataacgccgc cagcattttg
    12841 tgggtcgatt gcgtttcgaa gatcactttt cccgcaacac gctcgccgct cataccactt
    12901 ttcccctggt agatcggatg aaaatgggtg tacggcaccc aggcggaatc gaagtggatc
    12961 gacgggacat ccagcgtctg tttgatccag tcggtgttgt agagcaagcc atcataggtt
    13021 gagttggtaa tcaccgcatg aaccggccat tgtgcctgcg tggtggcaac gactttctct
    13081 tcgatgctgt cacgagtaaa ttcacggcgc gggatcccac ccagaatccc caacgcatta
    13141 cgcgtcggtt tcagccagac tggcactaca tcgttcatca tcaacagatg cgccagtgat
    13201 ttatggcagt tgcggtcgat caacagcgta ctgccggatg gcgcggcgta catacccaca
    13261 attttgttcg acgtcgatgt tccgttggta acgatataac tctgttccgc gccgaaagtc
    13321 cgcgcgatgt actcttccgc ttccaggtgt ggcccggtgt ggtcgagcaa cgaaccaagc
    13381 tcggtgaccg aaatagatac atcagcctta agagtattcc cgccgaaaaa atcataaaac
    13441 agacagccaa ccgggctttt ttgatatgcg gtgccgccca tatgccccgg cgtacaaaag
    13501 gtgtacttcc gctctttgac gtaggtaaac aaggctttcg tgaacggcgg tgtaatgtta
    13561 tcaagatatt cgtcggtgta ctgacgcata cgaatggcga tatcttccgc ctgccccagc
    13621 gcatattcaa aaaaccagag cgccatccgc atatcctgca cgctgacatc catcgtcgag
    13681 tgggtgttga tgaaggcata aagcgggaga tattcattaa gctgattgat atcgctacat
    13741 aaatcgagac tgtactcatc ccagtcaaaa atcacgccgc aaattcgagg gttatgttcg
    13801 ataaatttca gcaaatcaac gctgttttgt ggccagataa tctgaaagcc ttgcgccacc
    13861 agcgccgact ccagttcttt gatgggctca tctttataaa agacgccatg cggtcccata
    13921 atggcaatga tgttcatgtg ttcctcctgg aaaatccttc cttaatcata gcctgctcaa
    13981 accgtggcga taaaaaaggg ccaccgaagt gacccttttt cagaactttt gcgaattacg
    14041 cgtaaccgta gctcatcagg cgctgataac gacgattttt taaatcttca gtgcttaaca
    14101 cgtcgagatc ggccagatcc gccagcagtt gcgctttcaa cgatgccgcc atcgcttccg
    14161 ggttacggtg agcgccgccc agcggttccg ggatgatgga gtcgatcagt ttcagctctt
    14221 tcagacgcgg agcgatgata cccatcgctt cagccgccag cggcgctttg tcggcgctct
    14281 tccacagaat ggacgcacaa ccttccggcg agataacgga ataggtgctg tattgcagca
    14341 tattcacttt atcgcccacg ccaatcgcca gcgcaccgcc agaaccacct tcaccgataa
    14401 ccgtacaaac taccggtacg ccgaggcgag acatttcacg caggttgcgt gcaatggctt
    14461 cagactgtcc acgttcttct gcgcccacgc ccggataagc ccccggggtg tcgataaagg
    14521 tgatgatagg catcttaaag cgttcagcca tttgcatcag acgcagtgct ttgcggtaac
    14581 cttctggcgc tggcatacca aagttacggc gaattttttc tttggtttca cgaccttttt
    14641 gatgaccaat gatcatcacc ggacgaccat cgagacgggc gataccaccg acgatagctt
    14701 tatcgtctgc atacgcgcgg tcgccagcca gttcgtcaaa ttcatcaaat gccaggcgaa
    14761 cgtaatccag ggtataagga cgctgtggat ggcgtgccag ttgcgcaatc tgccatgcac
    14821 cgagatcggc gaagatttta cgcgtcagtt ctacgctttt ttcacgcaga cgatgcactt
    14881 cttcatcgat gttaatatcc agtttctcat cctgacggct aaccgcagtc agagaatcga
    14941 ttttcgcttc cagctctgca atcggctgtt caaaatcaag gaaattcaga ctcatagtat
    15001 tcctgtatta gtcaaactcc agttccacct gctccgaacc aatgaggcca cggagatcgt
    15061 ttaataaacg atcgctcgga gagacacgcc acgtcgcgcc aaaacgcaac cgcgcgcgtg
    15121 catccgccct ctgatagtag agatgtactg gaattgtccc agagcggtgg ggttccagag
    15181 actgacggag tcggtttaaa agctggtcat caatttgcct gtccgtcagc gagatagcaa
    15241 gcccgcgagc atatttttcc cgggcttcgt caatatccat cacttcgcga gcggtcattt
    15301 taagcccacc gctgaagtca tcaaagctga cctgtccgct gacgataagt atgcggtctt
    15361 tttccagcaa ttgctggtat ttatccaggg cgtcagtaaa caacatcact tccagccgcc
    15421 cggaacggtc atccagcgtg cagataccga tacgattgcc gcgcttggtg accataaccc
    15481 gcgcggcaac aacgagcccc gcagccgtga tgactttacc acgttctgtc gggtgcatgt
    15541 ctttcagcct tacgcctccg acataacgct caatctcttt taaatactgg ttgatagggt
    15601 gtcctgtcag gtacagacct aacgtttcac gttccccatc taataccacc tgctccggcc
    15661 acggttggca gctggcgtag gattgttcaa tttgttccgg ctcttcggcc agcacgccga
    15721 acatatcggc ctgaccgatg gcttccgctt ttgcgtgttg atcagccgct tttaacgcat
    15781 cgcccagcga gttcatcagc gctgcgcgat gtggcccaag acggtcaaat gccccggaca
    15841 tgatcagttt ttccagcacg cgacggttta actttttggt gtcggttcgg gcgcagagat
    15901 caaacagttc gcggaagtag ccgcctttat tacgggcttc gatgatggcc tcaatcggac
    15961 cttcaccgac ccctttgatc gcgccaatac catacacgat ttcgccgtcg tcgttgacgt
    16021 ggaaatggta aagaccggag tttatatctg gtggcaggat tttcagcccc atccgccagc
    16081 actcatccac caggcccacc accttctcgg tgttgtccat atcggcggtc attaccgccg
    16141 ccataaactc tgccggatag tgcgctttca gccataacgt ttgatatgac accaaagcat
    16201 aggccgcaga gtgcgatttg ttaaatccgt aaccagcgaa tttttccacc aggtcgaaga
    16261 ttttcatcgc cagttcggcg ttgattccgt tcttttctgc accttcagca aatacagaac
    16321 gttgcttagc catctcttcc ggctttttct tacccatcgc acgacgcagc atatccgcgc
    16381 caccgagggt ataaccagaa agcacctggg caatctgcat gacctgttcc tgatacagga
    16441 tgatgccgta ggttggctcc agtaccggtt tcaggctttc atgctgccac tgtacgtccg
    16501 gataggagat ctcttcgcga ccatgtttac ggtcgataaa gttatccacc atccctgact
    16561 gcaacgggcc ggggcggaac agtgccacca gtgcgatcat atcttcgaag cagtcaggtt
    16621 gcagacgctt gatcaggtcc ttcatgccgc gcgattcaag ctggaatacc gccgtggttt
    16681 ccgagcgttg caacatgtcg aagcttttct tgtcatccag cgggatcgcg gcgatatcca
    16741 gcggcggctc gccattcttc gcccgccgct tgttgatcat ctccagcgcc cagttgatga
    16801 tggtgagcgt acgcaaacca aggaagtcga acttcaccag tccggcgtat tcaacgtcgc
    16861 ttttatcaaa ctgggtaacc ggatgtttgc cctcttcatc gcaataaagc ggcgcaaaat
    16921 cggtaatttt ggtcggcgcg ataaccaccc caccggcgtg cttaccggcg ttacgggtga
    16981 ccccttccag tttgcgcgcc atgtcgatca gcgccttaac ttcttcatcc gcttcgtaga
    17041 tttccggcag ctgcggctcg gcttcaaacg ctttcgccag cgtcatcccc ggatcgggcg
    17101 ggatcagttt cgagatacga tcgacaaagc cgtacggatg ccccagtaca cggcctacgt
    17161 cgcggatcac cgctttcgcc gccatcgtac cgaaggtgat aatctgcgat accgcatcac
    17221 gaccgtacat gtccgctacg tgttcgataa cctgatcgcg tttctccata cagaagtcaa
    17281 cgtcgaagtc aggcatggag acacgttccg ggttaaggaa acgttcgaac agcaggtcaa
    17341 attccagcgg atcgaggtcg gtgattttca gcgcgtaggc caccagtgaa cccgcaccgg
    17401 agccacggcc tggccctacc ggtacgccgt tatctttcga ccactggata aattccataa
    17461 cgatgaggaa gtagcccggg aagcccatct ggttgataac ctgaagttca gtctccagac
    17521 gttcgtcata ttccgggcgg cgcttaacac gttcttcctc gtcagggaat aaaaaggcca
    17581 gacgctcttc caggccctct tttgcacgct tgaccagata atcttcggtg ctcatgtccc
    17641 cggtcgggaa ctgcggcagg aagtattcac caagacgcac ggtcacgtta caacgtttgg
    17701 cgatctcaac ggtgttggca agagcttcgg ggatgtcggc aaacagctcg cacatctcct
    17761 cttcgctacg catatattgc tgcggcgaat agttacgcgg gcgtttagga tcgtcgaggg
    17821 taaagccgtc gtggatcgcg acgcggattt cgtgtgcgtc aaagtcgctg ctgtcgataa
    17881 agcgcacatc gttagtcgcc acgacgggca aaccgcgcgc ttccgccagt tccaccgccg
    17941 cgtgcagata gctttcttca tccggcctgc cggtgcggat cagctcgaga aaatagcgat
    18001 ccgggaagtg ttcttcataa aacgcgacac actcatctac cagcgcgctg ttaccacgca
    18061 aaagactgcg tccgacgtcg cccatgcgcc cgccggaaag aaggatcaac ccttcgttta
    18121 attcgataag ccagtcgcga tcgatgatcg gcccggcggc accgtacccg cgctgatacg
    18181 cttttgagat cagcaacgtc agattctgat agccggtatt gttcgccgcc agtaccgtca
    18241 ggtgggttaa ctcatcaccc agcaggtcgc actggacgtt aaaatctgcc ccgacgatag
    18301 gcttaatccc tgcgccatgt cccgctccgt agaacttcac cagaccgcaa aggttggtga
    18361 aatcggtgat cgccagtgct ggcataccca acgccgccgc cttttttacc aacggtgcgg
    18421 ttttggccag gccatcgatc atcgagtagt cgctgtgcac ccgcaggtgt acgaaacgtg
    18481 gttcagacat cttcagattc cggtttactt aatctcgaca caagaatcag gacgcaagtc
    18541 ccagtgcgcg tttgacaggc ccaaagctgc gccgatggtg ttcggtcgcg ccgtgttcag
    18601 ccagtttttc cagatgaaaa gcggttggat accctttgtg ttgggcaaaa ccatattgcg
    18661 ggaaaacaat atccagcgcc gccatttcgg cgtcacgcgt cactttcgcc aggatagacg
    18721 cggcactgat ttccggtacg cggctatcgc ctttcaccac tgccatcgaa ggcatcggta
    18781 atttcgggca gcggttacca tcaatcaaca catattccgg cgcaatatgc agcccagcga
    18841 cggctcgctg catcgccagc atggtcgcat gtaaaatatt cagttcgtcg atttcgtgag
    18901 gttccgcgcg gcccagactc cagctcaacg ctttctcttt gatctcttca tagagcgcca
    18961 gacggcgttt ttcgctcagc tttttggaat cattcagccc ggcaatcggg cgcgccgggt
    19021 caaggatcac cgcagcggtg acgaccgcgc caactaacgg cccgcgtccg acttcatcca
    19081 cacccgcaac cagctgcgtg tgcggataga caaattcgat cattgtgcta actccagaac
    19141 ggcttgtgcc gcctgctcat cggcattgca gcggatctgc tgatgcagtt cacggaaggt
    19201 atcgtgcatc gcgtggctgg ttttcccgtt cgccaacagc ggtaacagcg ccgcagccag
    19261 tttttgcggc tcacactctt cctgcaataa ctctttgact aactctctgc ccgccagcag
    19321 atttggcagc gagacataat cagttttcac cagacgcttc gccaaccaga aggtgaaagg
    19381 cttcatgcga taccccacca ccattgggca tttcgccagc atacactcca gggctgccgt
    19441 acccgacgcc agtagcgccg catcactggc gaccatcgcc tcacggccca ttccatccag
    19501 caaatgaacc gacaggtctg gcgcaacttc cgctttgata cgctcaaact gctcacggcg
    19561 tttggcattc accagcggca ccacgatctc gagatccgga tatgtctggc gcaaaagctg
    19621 ggccgttttc aggaaatcgg cactaagcat ttcgacttct gcaccacggc tgcccggcaa
    19681 caatgccaga cagtgggcat cgtaagggat ccccagcaca tcacgggcac tatttttatc
    19741 tggatctaat ggcatggcat cagccatggt atgaccgata aagcggcacg gtacgttgta
    19801 tttgtcataa aacgcttttt cgaaaggcag aaatgcgagc accagatcgg tggctctgcc
    19861 tattttgaaa acgcgtttct gtcgccacgc ccagacggat ggactgacgt aatgaatggt
    19921 tttgataccc tgctttttga ggttaccttc aagagtaata ttgaagtcag gcgcatcaat
    19981 accaacaaaa acatctggct tcagttcgcc aaaacgcttt gtcagatcgg cacgaatatg
    20041 cagtaagcga cgcagacgac cgagcacttc aacaatgccc atcaccgcca gttcttccat
    20101 ttcgtaccag gcttcgcagc cttcagcctg cattcgtggc ccggcaacac caacaaagcg
    20161 ggcgttgggc acacgttctt tcagagcgcg gattaaaccg gcccccagga tatcgccgga
    20221 ggtttctccg gcgaccaggg caatagttaa tggacgctgt tcagtcatta acgaatcaga
    20281 ccgcgcgttg agcgtgcaaa gaaatcggta aaggctttca cttccggata tgtttccgcc
    20341 agttcagcaa tttccggttt cacttcatcg agcgttttac cgctacgata aattagctta
    20401 tacgcattgc ggatagcggt aattgcctca cggctgaatc cgcggcgctt cagcccttcg
    20461 atattgacac cgaacggcgt tgcgtggtta ccctgcgcaa tgacataagg agggacgtcc
    20521 tgcgccacac cggagcagcc gccaaccatc acatgcgcac caatgatgca gaactgatgg
    20581 actgcggtca tgccgccgat gatcgcgaag tcgtcaaccg atacgtgacc cgccagcgtt
    20641 gcgttgttgg cgagaataca gcggttacct accgtacaat cgtgcgcaat gtgcgcgttg
    20701 atcatcagta agttgtcgct gcccaccttc gtcaatccac cgccctggac tgtgccacga
    20761 tgaatggtga cgctttcgcg aatgcggtta cgatcgccga tttccacacg ggtcggttcg
    20821 ccagcatatt tcaaatcctg gttaacttcg ccgatggagg cgaactgata aatctcatta
    20881 tcgcggccaa ttttagtatg accattcacg acaacgtgag atttcagtac ggtaccctca
    20941 ccaatttcga catggggtcc aacgatacaa aaaggaccaa tgtgtgcgtt cgcgccaatc
    21001 gacgcgccct cttccacaat ggcggttgga tgcacaaagg cggatttatc aatcacgtat
    21061 caggcctccc ggctacgagc acacatcatc gttgcttcgc aaactacttt accgtcgacc
    21121 agagcaaccc ctttaaaacg ggtcaggccg cggcgcgttt tttcgaaagt gacttccatg
    21181 atcatttgat cgccaggcac gaccgggcgc ttgaagcgcg cttcgtcaat accagcgaaa
    21241 tagtacagct cacccggttc cagttttcct acgcttttaa acgccagaat acctgttgcc
    21301 tgtgccattg cttccagaat cagcacaccc gggaaaatcg gttttccagg gaaatggccc
    21361 tggaagaatg gctcattgac agagacattt tttactgcgc gcagaaaacg accttcttca
    21421 aaatccagca cgcgatccac cagtaagaaa gggaaacggt gcggcagaag ttctaaaatc
    21481 tcttcaatct gcagagtatg agtgttagta gtcaagatac tcttcctgtc aaaatataag
    21541 aaacgacaat aataacacgg cctgccgcaa tcgtaagaat gagacaggcc gtaaagtttg
    21601 gcgaacaaaa gatggaacgt tagtcttgtt gattaacctt gcgctcaagc gatttcagac
    21661 gcttgctcat gtcatcaatg ttcatcacca gtgcagcggt tttgcgccag actttgttgg
    21721 gttgcagcgg aatgcctgag gaatagacgc ctggttcagt gatgggacgc atcaccatac
    21781 ccatgcccgt aaccgtcact ttgtcgcata tttccatatg cccgttgatt acgctggctc
    21841 cgccgatcat gcagtaacga ccaattttca ggctgcccgc cataatgacg ccaccggcaa
    21901 ccgccgtatt gtcgccaatc acgacgttat gtgcaatctg gcactggtta tcaatgatca
    21961 cgccattgcc aataatagtg tcatccagcg cgccgcgatc gatggttgtg caggcaccga
    22021 tctccacgcg atcgccaata attacgcgac caatctgtgg gatcttcacc cagttaccac
    22081 gatcgttggc ataaccaaag ccgtctgcgc ctaccactgt tccggactgg atcaggcaat
    22141 tctgaccgat ctggatctca tggtaaatgg ttacgttcgc ccagagacgc gaacctgcac
    22201 cgattttgct gtttttacct acgaagcaac cggcaccgat aatcacgtta tcgcccagtt
    22261 caacgccgga ctcaatcacc gcgttagcgc caatcgatac gttgttaccc agcttcgccg
    22321 tcgcgtcgat caccgcactg ggtgcaatgt tctgcgcggg ctgcggcgtg gtatctaaaa
    22381 tttgcgccat gcgcgcgtaa gtcaggtagg gattcttcac taccagcgcg gcacttttcg
    22441 cgaaaggaag atcgtcctgg gtcatgacaa ccgcggacgc ctggcacaag cctaaatgct
    22501 cacggtattt tgggttaacc atgaacgtaa tgtgacctgt ttgtgcagat tgcatggacg
    22561 caacgccggt gatgacgata tcgccatcac cgtgtagttc tgcatccaac tgctgcgcta
    22621 aatcagccag tcgaattgaa ggcattactt atttaacctg tttcagtacg tcggcagtga
    22681 tgtcttttac atcgctgctg ttgtaagcaa cggcgtttgc atcaacaacc agatcgatat
    22741 cctggctgtt ggcaacggat ttcacagcag tctggatacg agtaaccagt ttgccgcgtt
    22801 cttcgttgga acgacgtgcg cgatcctgct caaaagcctg cgctttctga gcaaaagtct
    22861 ggcgctgagc catcacgtct ttttccagct tagtgcgatc gctgcccgct ttcatggact
    22921 gcagcttttt cattttagcc tgcagatcgg tttccatacg ctgcagttcg ctggcacggc
    22981 ctttgaactc attttccagc gtgttagaaa caccggtttt ctgcgctacc tgctggaaca
    23041 ggctgcccat gttgacgatt gcaattttgt cagccgcctg agcagaagtt gccagtgcta
    23101 aaccgagacc tgcagctaat aaccactttt tcacaataaa ctccttacca tcccatttgc
    23161 accggagggt gcagttcttt gcgtggcccg gcgatcttat attgatcgcc taaagtcatc
    23221 gctacactac cactacattc ctttgtggag aacacttacc aggttttacc gatgttaaac
    23281 tggaactgtt ctgccttgtc tccatcgtac tttttgaacg gctgggcgta ggagaacacc
    23341 aacggcccca atggggacat ccattgtaat gcgatacccg cagacatacg gatattgctt
    23401 ggatcactat agtccggata tccagaatat tggctggaat cccagtttgt atcccaaacg
    23461 gtacccatat cccagaagaa ggaagtacga accgagttag catacttatc gctaataaac
    23521 ggcgtcgggg tgatgaactc gaggctggca accgccatgg cgttaccgcc tacagcatca
    23581 tccgatttac acaggtcttt cgcgccgtcc tgagtcgcac attcgtaatc atagtccgga
    23641 tcataattac tggcctgatg cgggaagtaa actgctttcg gaccaatggt attggactgg
    23701 aagccacgca cggtgctgga accaccggca tagaagttct cgtagaacgg catctctttg
    23761 ccgcctaaac catcaccata accccagcgg gtacgcccca gaacaaccca tttgtgatcg
    23821 tcatcgatcg gcacataagt cgccgtgtct aacgtcactt tgtagtattc gttatccgat
    23881 ccaggaatgg tcactttacc ggtcaggttg acacgtgaac catctgtcgg gaagtaacca
    23941 cggtcaagct tgttataggt ccaaccatag ttgaacgtga agtcgtccgt tttgaagctg
    24001 ttatcctgat cagaggtgct cggatgttca cccatagagt acagataacg ccacatcgca
    24061 acctgaggct gcatgttgga cagggagtta tgtacataac ccagacctgc acgcagcgag
    24121 ttatattcgt tgatcgggaa gcccaacgtc acgtctgtac cataactctt gttggtatag
    24181 tcggacaggt cggcgtcatc tgcctggaag tcattataga agagacgacc accgaggctt
    24241 acaccatcta cagtgaagta cgggttggtt accgacaatt cagcataggt ctggtaatcg
    24301 tttttggtcc cgttgatacc aacagcataa cctgtaccta accagttatc ctgttgcacg
    24361 ccagcctgga agctcacgcc actttcagta ccgtaaccaa tcccgaagtt gaagctaccg
    24421 gtgttacgtt ctttcacctt gtagactaca tctacctggt ccgggctacc cggaacacgt
    24481 tgggtatcgg tatcgacagt ttcaaagaag cccagacgat tcagacgctc cttaccctga
    24541 tcgaccagat cgcttcccag ccatgcacct tccatctgac gcatttcgcg acgcaggacg
    24601 gcatctttcg aggtatcgtt accttcaaaa cggatcttac gcacgtagaa acggttaccc
    24661 gcatcaacgt tcacgcgtaa tttaacggtt ttgtcggcat cgttaatttc aggcatcgac
    24721 tgtacgcgcg gataggcata accatagcga ccgagaagct ttttgatgtc atcttccatc
    24781 ttggtcactt tggtgccgtt atacagctcg cccggctcga tcttagtcag ctgctcaatt
    24841 tcagcggagt gcccggcaag gttgccgctc acttcaacgc cagaaagctt gtactgatcg
    24901 ccttcggtga tgttcaccgt gacgtaaata ccttttttat ctggcgtcag actgacctgg
    24961 gtagagtcga tgttgaaacg ggcataaccg cggtccagat agtagctgcg cagggtttca
    25021 aggtcgcccg ccagtttctg tttctggtat ttacgatcgc ctaccacgtt ccaccacggc
    25081 acttcgtcac gcagttggaa atgagagatc agttcgtcgg tggtgaaagc atggttacca
    25141 acaatgttaa tttgctggat ttcagctgac acaccttcct ggaacaccag ttttaggtca
    25201 acacggttgc gcggcagcgg ggtcacgaca gctttaacgc tggcgctata tttaccgacg
    25261 ctgtagtaga agtcttccag acctttctcg atatcggcaa tggtggtgcg atcgagggat
    25321 tcgcccacac gcacaccaga agcctcgagg ttttgcttca gcatgtcatc tttcaccgat
    25381 ttgttaccgg agaaagtaat gctggcaatg gtcggacgtt cttttacctg aaccagaagg
    25441 gtatcaccat cacgaaggac gcgaacatcc tcaaagttgc cggtagcaaa cagagcgcga
    25501 atggtattac tgatatcttc atcattaacc gtgtcgcctg tgcgcaccgg catactgagg
    25561 agggccgcac caacggcgac acgctgaagg ccttcgaaat gaatatcttt cactacgaac
    25621 ccttcagcac cgtatacggt ggcgctgcta aacagcagcg acgctatgag caactttttc
    25681 atcgccatcg ttattatgcg ttcttcctaa ctaactctca taaccgagag aaatcattga
    25741 aaagtgcaag ccccattaac agcaccagca gaatcgagcc aatgcgataa caaaagtctt
    25801 gaacccgctc ggataccggt ccgcccttga tcttttcgat cgcaaggaac agcagatgcc
    25861 ccccgtcaag tacgggcaac ggaaacaggt taattatccc taagttcacg ctaataagcg
    25921 caagaaacgg caggtaataa actaccccga gttccgctgt catcccagcc cccttggcga
    25981 tagagatcgg cccactgagg ttgttcagtt tcacatcacc ggtgatcaat tttcccagca
    26041 tactgaccgt cagcttcatc agctgccacg ttttgtccgt ggcttcgacg atggcgttga
    26101 acggcccata ctggcgtaca actttatact catctggcaa aggaatgact ttcggctcaa
    26161 taccgacaaa accaatcgct ttaccattac ccggtttact ctccgggatt aatgtcaaag
    26221 acaagggact cccctgcctt tcgatttcta acgctaagga tttacccggg ttatcccgga
    26281 caagcatcac aaaggtcacc cactgcgtta agggctgacc atcgacttta acgatcctgt
    26341 cgcctgcttg caaacctgcc ttgcttgccg ccgagtttgg ctgcacattt tccagtacag
    26401 gttcaatttg cggcccacga ggacgaatcc ccagcgaaga taccggatct tctttatcag
    26461 gctcaaacgc ccagtgacgt aaatcgagct ttacatcccg ccgttggtcg ctgccaaatg
    26521 gcgctactgt aatggtggtg ctttcatcgc caattttatc gaccaactgc aaacgcacgg
    26581 catcccaatc aggcgtttcg ataccatcta cggcttttag ttccgtacct ggtgcaattt
    26641 gtgcttccgc agctatcgaa ttggctgcta tttcgccaac caccggacgt acgccaggca
    26701 caccaataat aaaaaccagc cagtaggcaa agatagcaaa aatgaagttt gcaaccggac
    26761 ctgcggcaat aatcgccgct cgttggccga cagatttatt attgaaggca tggtggcgga
    26821 gttccggaac gaccggttct gcgcgctcat ccagcatttt gacataaccg cccagcggga
    26881 tcagggcgat aacatattcg gtgccgagct tatcagttcg ccgccagagc gccttaccaa
    26941 accctattga gaaacgctca acgcgaacac cacaacgccg ggcaacccag aaatgaccaa
    27001 attcatgcac ggtgataagt acacccagtg caacgatgaa cgaagccaaa tcccagagaa
    27061 aactcagcat attaccttcc gttaaagcgt cctgaatacc agtaacaaca agcaagcaaa
    27121 gaccggtacc gcagccgtca ggctatcaat acgatctaaa ataccaccgt gtcctggaat
    27181 taaatgaccg ctgtccttaa ttcctgcttc acgcttaaac atactctcgg tcagatcgcc
    27241 gagcactgag gctaacgctg cgacaataga gcaaatgagt aaggtgacgg gagcgacgtc
    27301 gagattcgcc cacatgccat aaccccatga gattaccgct gcggtcgcta gtccacctat
    27361 aaagccttgc caggttttac ccggagaaac cttcggtgcc agcttatgtt taccaaacaa
    27421 tttgccaaac atatatgcgc cagagtcagc gccccatacc aggatcatga catagagcag
    27481 ccatattgcg ccactgtaat gattctcgtc atagtgccag gcccgtaacg ccagcatgcc
    27541 ccagaagaag ggaacaatgg ttagcacgcc aaaaataagg cgcaatgttt tagagttacg
    27601 ccagattgct gcggaacctg ggtaaaacag caccagcaat agcgcgacaa tccaccaacc
    27661 caacgaagcc caaagtgaga tttcaaccag cggttgatga atatttcggt gatattccgg
    27721 caacagaaaa agcatcagcg ccaacaataa cccgcataac accgccaacc atactcgctg
    27781 cgaacgagtg gtaaaaccgc taagctgtcc ccattcccac gctgccagca tgcagaccac
    27841 cagcgttaca atggcgaacc ccaccggcgg caacagaaac aacgccgcga tgacgacggg
    27901 tattaacaca aaagcagata tcaggcgata cttcagcaaa agcgaccccc atcaggctgt
    27961 ttcatcaccg ggctcggtgc cgccgaaacg acgctctcga ttagcaaagg catttaacgc
    28021 cccttcaaag tcttgttcat cgaaatcggg ccagagaaca tctgtaaagt aaagttcggc
    28081 ataggcaatt tgccaaagca aaaagttact aatgcgatgc tcccccccag tcctaattac
    28141 taaatctaca ggggccagtt catgcataca gacatgctgg tttagcatct cttcatctat
    28201 ctgatctggt tgcaggtttc cttgctgcac cttttcagcc agttgcctga ctccctggac
    28261 tatatcccaa cgtccaccgt agttcgccgc aatattcagc gtcagaccgg tattcccggc
    28321 tgttagcgct tcagatttac gaatacgttc ttgcaaacgc gagttaaagc gactggtatc
    28381 gccaataata cgcagacgca cgttatgtcg gtgcagactt tttacttcgc tatcgagcgc
    28441 ccacacaaac agttccatta acgcactgac ttcctgcgct ggtcggttcc agttttcact
    28501 actaaaggca tacagcgtta acgcctcaat accgttgttg gccgcaaaag agacagcccg
    28561 gcggacggat tttgccccgg ctttatgccc aaaggcacga atcttccctt gcttttttgc
    28621 ccagcgacca ttgccgtcca taatgatcgc aacatgacgg cagccatgcg ctggcaattt
    28681 ttcgctaagt ggttgagtag cagacaacat cacgcgtttt ttattccctg acagggttta
    28741 cggtactcag gaatactgaa gcctatacaa aaaaaagccg tgtaaaacca cggcatacct
    28801 gaccttataa agccaactac ttacgatcag atggcgcaga ctatatcact gaagccctac
    28861 gctaacaaat agcgcgactc tctgtagccg gattatcctc agcttgcgag acgcatcacc
    28921 tcttttctgg cggcttcacg cgcgttcgca tcaacagata acacatcgtc cacacattgt
    28981 ggttcgcgca tatccatttt ttccagtacg gataaattca acgcagcgat atccgtaaag
    29041 cggatttgtt gcgcaagaaa agcagcaacg gtgatttcgt ttgcggcatt caatgctgtc
    29101 gtcgctgcct ggccttgttc gaacgcctcc atcgccagtt tcaggcatgg ataacgatca
    29161 taatccggtg cggcaaatgt caacgcactt agtttgcaaa aatcgagcgg cttcacgcca
    29221 gagttcacgc gattcggcca tgccatggtg tgggcaattg gcgtacgcat atccggttcc
    29281 cccagctgcg ccagaacact gccgtcctga tagcgcacca ttgagtgaat cactgactgc
    29341 gggtgaatca gcacttccat ctggctggcg ctggcgttaa acagccaacg cgcttcaatg
    29401 tattccagac ctttgttcat catggtagcc gaatcgacag aaattttacg tcccatcgac
    29461 cagttcggat gacggcaggc ttgatcgggc gtcattgttg ccaaatcgcg caatggcgtc
    29521 tcacggaaag ggccaccaga cccggtaagt aaaatggaca ccacgccatt ttgctcaagg
    29581 tcagcgtatc ccagattatg ctggataggt tgcggtaaac tctgaaaaat ggcgttatgt
    29641 tcgctatcga ccggtaacaa ttgcgctttg ctctgcttta cggcgtccat aaacagacgt
    29701 ccgcaggtaa ccagtgattc tttattggcc agcaaaatgg ttttacccgc gcggatcgca
    29761 gcaagcgtag gtaacagccc agcagcgcca acaatggctg ccatcacctg atcaacatcc
    29821 tcaagcgctg ccatatcgca agcggcttgt tgcccactta agacttcggt gcggctaccc
    29881 tgttgctgta gcatcgtttt aagaagtttc gcactcgctt catcgtccat tacggcatag
    29941 cggggagaga attccaggca ctgttctacc atgcgagtga catttttgcc tgccaccagc
    30001 gcaactacgc ggaagtgttc gggattatgg cgcaccacgt ccagcgtgct gcaaccaatc
    30061 gagccagtcg agcccagaat ggtgagttgc ttcatgaaac atccagagtt gagacagaat
    30121 aaaaagcaaa acgccgccag ccgatccgta aggatctact gagcggcgtt tttttgtcgt
    30181 tcaagaaatc agaactgcat cagttctgct tctttgtctg ccagcgccgc ttcaattttc
    30241 ttgattgcag catcagtcag tttctgtaca tcgtcctgag aacggcgatc gtcgtcttcg
    30301 ctgatctctt tatctttcaa cagtgctttc actttgtcgt tcgcgtcacg acgcacgtta
    30361 cgtactgcaa cacgcgcttg ttctgcttca ccacgaacga ttttggtcag atctttacga
    30421 cgttcttccg tcagcggcgg cagcggaaca cggatgtcgc tacccgcaga gttcgggttc
    30481 aggccaagat cggacgccat aatcgctttt tcaacggccg gagacattga acgatcaaac
    30541 acgttgattt tcagagtacg ggaatcttct accgttacgc ttgccagctg acgcagcggc
    30601 gtcggcgtgc cgtaatattc cacgacaatg ccatccagca ggctgggaga agcacgaccc
    30661 gtgcgtattt tgctgatttg ggttttgaac gcttctacgc atttgtccat gcgtacttca
    30721 gcatcttttc tgatatcgct aatcacgtta cgaatccttg aaaacttgtc tcagtcagac
    30781 cagacagtca cacacagtgg aagtgtgcta agtatagccc tgattaaaca tattacgggt
    30841 aatcgtctgg attattaggc tattttattt gccattttgg ccccgagcag tgctcgccat
    30901 cctcacgtac ttttgtacgc tccggttgct gcgcgctggc cgtgaccaaa ctgcctgcaa
    30961 caataacgcc ttataaccag acttacgccc ccgcgatact tacgcggaat cttaccctta
    31021 tttatccatc acgggaatta ttccgtgatt aaagtccctt ctttttcacc cattaccaca
    31081 cggcgcagcg cacccggttt gttcatgttg aaaacacgaa tcggtaattt atggtcacga
    31141 gccagcgtga aggccgccag gtccatgact ttcagctctt tttccagcac ttcgctgtaa
    31201 gtcagttgct cgtacatggt tgcagttgga tctttcgccg gatcagcggt aaacacgccg
    31261 tcaactttgg ttgctttcag caccacatcg gcttcaattt cgataccacg caggcaagct
    31321 gctgagtcgg tggtaaagaa cgggttacct gtaccggcgg agaggatcac cacacggttg
    31381 ttgcgcaaca ggctgatagc ttctgcccag ctgtagctgt cgcacacgcc attcaatgga
    31441 atagcggaca tcaggcgagc gttcacatag gcgcggtgca gtgcatcacg cattgccagg
    31501 ccgttcatta cggtcgccag catccccatg tggtcgccca caacgcggtt catacccgct
    31561 ttcgccagac cagcgccacg gaacaggtta cccccaccaa tcaccacacc aacctgaata
    31621 cccagttcaa ccagttcttt gatttcctga gccatgcgat ccagtatgct tgcatcaata
    31681 ccgaatcctt cagtgccctg cagagcttcg ccactcaact taagcagaat gcgtttatag
    31741 acgggttttg cattggtagc catgtttctt tcctgagact gtcaacgatt gaaatgaatt
    31801 aatactagcg acattgtacg tcgcttttca gcgcagcccc acaagggtta gctgaattta
    31861 caagatgggc acaaaaagaa gccgccctca ggcggctcct tttcgataat taagactgct
    31921 tggacatcgc agcaacttct gctgcaaagt cagtctcaac tttctcgatg ccttcaccta
    31981 cttcgaagcg gatgaagcca gtcacttcag cgttatgctc tttcagcagc tgaccaacag
    32041 ttttgcttgg ttccataacg aacggctgac cggtcagaga aacttcgccg gtgaatttct
    32101 tcatgcggcc ttcaaccatt ttctctgcga tttctttcgg cttaccagac tgcatcgcga
    32161 tatccagctg tacctggtat tctttctcta ccacttcagc ggatacgtct tccggtttga
    32221 tgaattctgg cttgcttgca gcaacgtgca tagcgatgtg tttaaccagc tcttcgtcag
    32281 cgcctttagc agcaaccaga acgccgatac gcgcaccgtg ctgataagaa cccagaacgt
    32341 cgccttccag cgcagcaacg cggcgaatgt tgatgttttc accaattttc gctaccagcg
    32401 caacacgttc ttcttcgaac tgtgctttca gaacttcaac gtcagtgatt ttgccagcaa
    32461 cagctgcgtc cagaactttg tctgcgaacg cctggaaacc agcgtctttt gcaacgaagt
    32521 cagtctggca gttaacttcc agaatgatgc cgtagttgcc gtcgattttg gttttgatca
    32581 cgccgtcagc agcaacattg cctgcttttt tcgctgcttt aatagcaccg gacttacgca
    32641 tgttttcgat tgccagctcg atgtcgccgt tagcttcagt cagtgctttt ttgcaatcca
    32701 tcatgcctgc gccagtacgc tcacgcagct cttttaccag ggatgcggta atttcagcca
    32761 ttctaaaatc ctcggagatg tgatctgccc ggcattaaac cgcacagata taaaagtgaa
    32821 aaagggggcc atatgcaggc cccctaacca aacgtgtact acctggtcta taagggctcg
    32881 tacgagcgtg ccttattact cagcttctac gaagctttct tccgcctggg aagccagatc
    32941 ctgagaacgg ccttcacgta cggttgcagc aacagcgccc aggtacaggg tcacagcacg
    33001 gattgcgtcg tcgttacccg ggataacgaa gtcaacaccg tccggatcag agttggtatc
    33061 aacgatagca aataccggaa tacccaggtt gtttgcttct ttgatagcaa tgtgttcgtg
    33121 gtcagcatcg attacaaaca gagcgtccgg cagaccgccc atgtctttga taccgcccag
    33181 gctgttttcc agtttctcca gctcacgagt gcgcatcagc gcttctttct tggtcagctt
    33241 gtcgaaagta ccgtcctgag actgagtttc caggtctttc agacgtttga tggactgacg
    33301 aacggttttc cagttagtca gcataccgcc cagccagcga tggttcacga agaactggtc
    33361 gcagctcaga gcagcgtctt tcaccgcttc gcttgcagcg cgtttagtac caacgaaaag
    33421 gattttacct ttgcgagaag caatcttgtt cagttcagcc agagcttcgt tgaacatcgg
    33481 tacagttttc tcaaggttga tgatgtgaac tttgttacgc gcaccgaaga tgaacggctt
    33541 cattttcggg ttccagtaac gggtctggtg accgaagtga acaccagcct tgagcatgtc
    33601 gcgcatggaa acagttgcca tgattaaaac ctctatataa aagttggggt tatgcctcca
    33661 cgtatcccat attaccgacc ccaaagggca ccccggaata tgtgccgata cgtgtgtgtt
    33721 gttacacaaa gtgagattgt cgcttccgtc cagtctgtgt atacgaaatg gatcggaagt
    33781 ccggcgcgct ttataccaca aatacgccgt ggacaccaat aattgttggc gctgtgtaca
    33841 gcatcagacg tcgaattttc tattatagaa aaccttcagt ggcacgtttg gcgaaattca
    33901 gaatgattct caatttgcca gggtgtgata ccattgacgg cacttacata tatattgtcg
    33961 gtatcaccga cgctgatgga cagaattaat ggctatctca atcaagaccc cagaagatat
    34021 cgaaaaaatg cgcgtcgctg gccgactggc tgccgaagtg ctggagatga tcgaaccgta
    34081 tgttaaaccg ggcgtcagca ccggcgagct ggatcgcatc tgtaatgatt acattgttaa
    34141 tgaacaacac gcggtttctg cctgcctcgg ctatcacggt tatccgaaat ccgtttgcat
    34201 ctcaattaat gaagtggtgt gccacggtat cccggacgat gcgaagctgc tgaaagatgg
    34261 cgatatcgtt aacattgatg tcaccgtaat caaagatggt ttccacggcg atacctcgaa
    34321 aatgtttatc gtcggtaagc cgaccatcat gggcgaacgt ctgtgccgca tcacgcaaga
    34381 aagcctgtac ctggcgctac gcatggtaaa accaggcatt aatctacgcg aaatcggtgc
    34441 ggcgattcag aaatttgtcg aagcagaagg cttctccgtc gttcgtgaat attgcggaca
    34501 cggtattggt cgcggcttcc acgaagaacc gcaggtgctg cactatgact cccgtgaaac
    34561 caacgtcgta ctgaaacctg ggatgacgtt caccatcgag ccaatggtca acgcgggtaa
    34621 aaaagagatc cgcaccatga aagatggctg gacggtaaaa accaaagatc gcagcttgtc
    34681 tgcacaatat gagcatacta ttgtggtgac tgataacggc tgcgaaattc tgacgctacg
    34741 caaggatgac accatcccgg cgataatctc gcacgacgaa taagatgaag ccggcgaatg
    34801 ccggcttttt taatgcgata atttaatctt atgggtggcg cacaatgaat acccttccag
    34861 aacagtacgc aaacaccgct ctccccaccc tgcccggtca accgcaaaat ccatgcgcct
    34921 ggccccgtga tgaattaacc gtcggtggga taaaagccca tatcgatact ttccagcgtt
    34981 ggctgggtga tgcctttgac aatgggatct ctgcagaaca gttgattgag gcgcgcaccg
    35041 agtttatcga ccagctcctg caacgattat ggattgaagc gggattcagc cagattgccg
    35101 acctggcatt ggtcgccgtc ggtggctacg gtcgtggcga gctgcatcca ctttctgaca
    35161 tcgatttact gattttaagc cgtaaaaagc tcccggacga tcaggcgcaa aaagtgggcg
    35221 agctgttaac gctgctctgg gatgtaaagc tggaagtcgg tcatagtgtg cgcacgcttg
    35281 aagagtgcat gctggaaggg ttatcggatt taaccgtcgc caccaatttg atcgaatccc
    35341 gcttattaat tggcgatgtt gcgctatttc ttgaactgca aaaacatatt ttcagcgaag
    35401 gattctggcc ttccgacaag ttctacgcgg cgaaagttga agaacagaac cagcgccatc
    35461 agcgttacca cggcaccagc tacaatcttg aaccagacat caaaagtagc cctggcggct
    35521 tgcgcgatat ccacactctg caatgggtgg cccgccgtca ttttggcgca acatcgctgg
    35581 atgaaatggt tgggtttggc ttcttaacct cagcggagcg ggcggaatta aacgaatgtc
    35641 tgcatatatt gtggcgtatt cgctttgccc tgcatctggt cgtcagccgt tacgataatc
    35701 gcctgttatt cgatcgccag cttagcgtcg cccagcgtct gaattacagt ggcgaaggta
    35761 acgaaccggt cgagcggatg atgaaggatt acttccgcgt gacacgccgc gtcagtgaac
    35821 tcaaccagat gctgctgcaa ctgttcgatg aagccatcct cgcccttccc gccgacgaaa
    35881 aaccacgtcc aatcgacgat gagtttcagc tacgcggtac gctaatcgac ctgcgtgatg
    35941 aaacactatt tatgcgccag ccggaagcca tcttgcgtat gttctacacc atggtgcgca
    36001 acagtgcgat caccggcatt tactccacca cgttgcgcca gttacgccat gcccggcgcc
    36061 atctgcaaca accgctgtgt aatattccgg aagcacgaaa actgtttttg agcattctgc
    36121 gtcaccccgg agcggtgcgg cgcgggctat tgccaatgca tcgccatagc gtactcggcg
    36181 cgtatatgcc gcaatggtca catatcgtcg ggcagatgca gtttgatctg ttccacgcct
    36241 acacggtgga tgaacatact atccgcgtga tgctgaaact ggagagtttt gccagtgaag
    36301 aaacgcgcca gcgccatccg ttgtgtgtgg acgtctggcc gcgcctaccg tcaactgagc
    36361 tgattttcat cgccgcgctg tttcacgata tcgccaaagg acgcggcggc gaccactcta
    36421 ttctcggtgc tcaggatgta gtgcattttg ccgaactcca cgggctgaac tcacgcgaaa
    36481 cacagctggt cgcctggctg gttcgccagc acttgctgat gtcggtgaca gctcagcgtc
    36541 gcgatattca ggacccggaa gtcatcaagc agtttgccga agaagttcaa acggaaaatc
    36601 gtctgcgcta tctggtatgc ctgacagtgg ctgacatttg tgccaccaac gaaacgctgt
    36661 ggaatagctg gaagcaaagt ctgttgcgcg aactctattt tgccaccgaa aaacagctac
    36721 gacgcggaat gcaaaacacg ccggatatgc gcgaacgggt tcgccatcac caactccagg
    36781 cgctggcact gctgcgcatg gataacatcg acgaagaggc gctgcaccaa atttggtcac
    36841 gctgtcgtgc taactatttt gtccgccata gcccaaatca actggcctgg catgcgcgcc
    36901 atttattaca gcatgattta agcaaaccgc tggtattgct tagtccgcag gctacgcgtg
    36961 gaggcaccga gatttttatc tggagcccgg accgccccta tctgtttgcc gccgtctgtg
    37021 ccgaattaga ccgccgcaat ttaagtgtcc acgacgcaca aattttcacc actcgcgacg
    37081 gtatggcgat ggataccttc atcgtgctgg aacccgatgg cagcccgctg tccgcagatc
    37141 gccatgaggt tattcggttt ggtctggagc aagtactgac acaaagtagc tggcagccac
    37201 cgcagccccg tcgccaaccc gccaaattgc gccattttac tgttgaaacc gaagtgacgt
    37261 ttttgccaac ccataccgac cgcaaatcgt tccttgagct tatcgcgctc gaccagccag
    37321 gactgctggc gagagtcggg aaaatttttg ccgatctggg aatttcgctt catggtgccc
    37381 gaattacaac cattggcgag cgagtagaag atttattcat aattgccacc gctgaccggc
    37441 gtgcacttaa taacgagttg cagcaggaag tgcatcagcg gttgacagag gccctcaatc
    37501 caaacgataa agggtgatgt gtttactgat atgaaaagag tttaacaatg cagcagttac
    37561 agaacattat tgaaaccgct tttgaacgcc gtgccgagat cacgccagcc aatgcagaca
    37621 ccgttacccg cgaagcggta aatcaggtga tcgccctgct ggattccggc gcactgcgtg
    37681 tagcggaaaa aattgacggt cagtgggtga cgcatcagtg gttgaaaaaa gcggtgctgc
    37741 tctctttccg tattaatgat aatcaggtga tcgaaggggc agaaagccgc tacttcgaca
    37801 aagtgccgat gaaattcgcc gactacgacg aagcacgttt ccagaaagaa ggcttccgcg
    37861 ttgtaccacc tgcggcggta cgtcagggtg cgtttattgc ccgtaacacc gtgctgatgc
    37921 cgtcttacgt caacatcggc gcatatgttg atgaaggcac catggttgat acctgggcga
    37981 ccgtcggttc ttgtgcgcag attggtaaaa acgtccacct ttccggtggc gtgggcatcg
    38041 gcggtgtgct ggaaccgctg caggctaacc caaccatcat tgaagataat tgcttcatcg
    38101 gcgcgcgctc tgaagtggtt gaaggggtga ttgtcgaaga aggttccgtc atctctatgg
    38161 gcgtatacat tggtcagagc acccgtattt acgaccgtga aaccggcgaa atccactacg
    38221 gtcgcgttcc ggcggggtct gtggttgttt caggtaatct gccgtcgaaa gatggcaaat
    38281 acagcctcta ctgtgcggtt atcgttaaga aagttgacgc gaaaactcgc ggcaaagtcg
    38341 gcattaacga actgctgcgt accatcgact aaaagtatgc acacgggcag cacgacgctg
    38401 cccgattgct ttctggttca gaataattcg tcctactcat tcttcctccc ccttttttaa
    38461 atactttttc atcttgtttc ggactgtcat gtaattcgca aaacgaagat aaatgtgcta
    38521 atccatccca tggaatatcg ccacaataaa gcaggtaatt ttttgatttt tacgccatga
    38581 tagaagtctg gaagaaaaag gctttctgcg atcacagtcg agccgggcgg gccaggccaa
    38641 atgctaaaat aagcacaatt tactgactca ttttgaggta acgctatgta cgacaatctg
    38701 aaaagtctgg gtattaccaa tcctgaagaa attgatcgtt acagcctccg gcaggaagcc
    38761 aacaacgata ttctgaaaat ctatttccag aaagacaaag gcgagttttt cgccaagagc
    38821 gttaagttta aatatccgcg tcagcgtaaa acggtcgtcg ctgatggtgt gggtcagggt
    38881 tataaagaag tccaggaaat cagcccgaat ctacggtata tcattgatga gcttgatcaa
    38941 atctgtcagc gtgaccgcag cgaagttgat cttaagcgta agatcctcga cgacttacgt
    39001 cacctggagt cagtcgtaac caataagatc agcgagattg aagccgatct ggaaaaacta
    39061 acgcgtaaat aatcccgaat tgacggttaa ctcgggcatt cccctctccc cttcgggctg
    39121 aggggaatga gccagcgcct cctcaacctt aacgcataac ctaccgctct tcatccagtt
    39181 gtaacgccac atacagcagc aacctgtcat caaaattgcc caaatcaagc ccggtcagtt
    39241 ccgatatacg attaagccga tactccaggg tattacgatg aataaacaac gcctttgacg
    39301 ttgccagcgg ttgcacattg tgacgaaacc acgccgccag cgttcgtcgc agcaagccgt
    39361 tattgtccat cgctttcagc cgcgccagcg gtcgcgccag ttcgttggcc tgccagtcgc
    39421 cacgcaaact gtcgagtaac acaggtaaca tcagatcctg ataaaaatag cagcgacttt
    39481 ctggcatccg ctgtttaccc accaccatcg tcgttttcgc cgtacgatag gatcgggcaa
    39541 tactgccagg accggtaaaa tagttgccca gtgaaacgcg aaaacgcagc tggccgtact
    39601 ctttcatgcg ggtaatcagt tgttcaactc gcttacgatg atcttctgca tcccagcgcc
    39661 caaaagagtt caacgccggt ttcaacacca ccatttcggt tagcgagaca atcgccacca
    39721 gattattacg ctcgggcgta gtcagcgcgt tttgcagttg ttgtaactcc gccattgcgc
    39781 tgtccacacc aagctgaccg ctgtcgacct caacaatagc caccactcgc ggttgattga
    39841 gatcgatccc cagccgttgc gcccattcag taagtgcggg agtattctcc tctgcctgaa
    39901 tcaggttcat caccagttcc tcccgcaaac ggctatcctg cgccaacaag tgcatcaacc
    39961 gcgactgttc cagcatcatt tcagccgtca tgcagaccag ttcgccatat ttacgcagat
    40021 tctctggttc acctgtcagg ccaattacgc cgacaatttc accttccagc cgtaacggta
    40081 gattaatccc ttgccgcaca ccgtgcagat gacgtgccac cgcgtcatcg atatcgacga
    40141 ctcgtccctg tgaaagtacc agcaatgcac cttcgtgcaa ttcaccaata cgctcacgat
    40201 cgccgctgcc gataattcgc ccacgggcat ccattacgtt gatattggta tcgatgatgc
    40261 gcatggtacg tgccacgata tcctgcgcca ttttggtatc aagatgccag ccagccataa
    40321 gtcctccgtt atgcacggct tagcataagg aagtacgtaa cgtacggcat tgtgcaaatg
    40381 cctaaaggat gatgaagatg tatggagttg tggtgaagtt cacagattgt aaggagaacc
    40441 ccttcccgtt ttcaggaagg ggttgaggga gattactgca ttaacaggta gatggtgctg
    40501 tcgccgcgct gaatgttcag tgccagcaca gacggtttgc tgtcgagaac tttacgcagt
    40561 tcagcgatgt ttttcactgc ctgctggttc gcgccaataa tcacatcacc tttcttcagg
    40621 ccgatctgcg cagccggagt gcccgttttc acgttgttca ctaccacgcc ctgatctttg
    40681 cctttgttgc tcatctcagc gccttcaatg ccgttgaaga tggagctgga atcaacctga
    40741 ttctggctgc tctgctgcag ttccagattc acgttgacct gcttaccgtc gcgcagcaag
    40801 cccagggtca gtttgctgcc caccggcata gtacccactt gagcacgcag tgcggcaaag
    40861 ctgctgatcg gcttaccgtt cagtgaggtg atcacatcac ccgctttaat gcccgctttt
    40921 gcagcggagg aattaggcag aacctggctt acgaaagcac cgcgctgggc gtcaactttc
    40981 atcgctttcg ccagttcgga gttcagctca gtccccataa tacccagctc accgcgtttc
    41041 acctggccgt attccaccat ctgcgaggtc aggtttttca ccatgttgct cgggattgca
    41101 aaaccgatac cgatgttgcc gccgtccggt gcgaggatcg cggtgttgat accgatcagt
    41161 tcgccgttca ggttaaccag cgcaccaccg gagttaccac ggttgatcgc tgcatcggtc
    41221 tggatgaagt tttcgtagtt ttcagcattc aggccgctac gccccagcgc agagacaatc
    41281 ccggaagtta ccgtctcgcc cagaccaaac gggttaccaa tcgctacggt gtaatcaccc
    41341 acgcgcagtg catcagaatc cgccatctta attgcggtca ggtttttcgg gttctggatt
    41401 tggatcagcg cgatatcaga gcgcggatct ttgccaacca tcttcgcgtc gaacttacgg
    41461 ccatcgctca gttgaacttt aatgaccgtc gcgttatcaa caacgtggtt gttggtgacg
    41521 acatagcctt tatcggcatc aatgatgacg ccggaaccca gcgccatgaa tttctgttgc
    41581 tggccgccac cattaccgcc ctggccaccc tggcagaacg gagagctctg gaacggagaa
    41641 ccttcctggc agaacggaga atcatcaccg aagaactgct ggaaattacg cggcatacgc
    41701 ggcgtattaa cggttgtgct accttctacg ttaatgctga ccactgaagg catcaccttt
    41761 tcgagcatcg gtgcaaggct tggcatctgc tgggctgtcg ttgctgaaga agtctcagcc
    41821 gccgttgcag agagcggaga taacgccaaa cctaaactca gagccagtgc actcagtgct
    41881 aatgtggttt ttttcatgta tttcagtctc gattaacaga taacgcaaaa ttgctgtgtt
    41941 cttcagattc gttttatagc gcgaagttcc gaactaaagt ttctgcaaaa ggtaaaaatt
    42001 tattgttcgt ctttacaaaa gcctggttat tgttctacgg ccatcagacg tcggtattca
    42061 tcccaggcat agaggtcggt cataccgctg atataatcct gcagcaggcg gcaacggtaa
    42121 taatattccc atagcggaaa ctcaggagaa tctgacggta atttactgac agcctcgaca
    42181 taggccagcc gatggcgcgt cgagagtttg tggaataagc gagattcaat agggaaacgt
    42241 ttcacccgtt ctttttctac cagttcagta aagtctgata atgacaggct taataaaggg
    42301 cgataaatct ctaataatcc gctaatgacc cgatagccct gcaattcaag ctgctcgaca
    42361 tctggatggc taaacacatg ttttacagcg acatttttat atagcttaag aagatcgctg
    42421 cattcgctgg catcttccaa taatgcatga ttaaacgttc cggcgaaaat cgcaggcaga
    42481 ttatcaataa atcgttgtgc cgcgtagggt accagtttat ttagggtgtt tacccgtaaa
    42541 tacataaaaa actgatcttc cgtactgcgg cttaaactat ttgagcgtga tttttcccag
    42601 gcattttcaa ccaccagcga aaagagcgaa cctttctcat gctggcccca cgcctcgtgc
    42661 aaatgatgat aaagctgctc aacggtaaat attcttttct ctaccgcatc ttcaagatct
    42721 gccacacaat aggagatgtc gtcggcagct tccataatcc acgttaatgg aaaacggctg
    42781 taaagcgcca aattaagttc tttacgcaac cgggcaatat aggcttcttc agaaagataa
    42841 taacccggct ttttcattaa atagtgatgt gtctcaggcg tttcgccacg ccaccacgcc
    42901 ggacgggtat attttaaaat accgccaacc tgtgcccagg tgagattcat cctcatcaat
    42961 gtatgcacca gacgaatgcc ttgtgcattc ccctcaaaat gacataagtc ctgacgaatc
    43021 ttgcgccgca gctcgttaag cggttcttcc ccgtcccgta aacgtagtgc cgccacgctg
    43081 cagcgatcgt cagtcagagg ctggctttcg gcatcttccg ggtgcaaacg ttggcgaaac
    43141 cagtcattta tcgctgcttc gccaaaatga ccaaacggcg gattgccgat atcgtgcatc
    43201 aggcatgaca tctcaacaat gctttcaaaa ggtccggtca gttcatccag gccgtatgct
    43261 tccagcaatt taagctcttt cagacggctt aaaatttctt tggcgatgta gcgccccacc
    43321 tgctggactt ccatcgagtg ggtaagacgc gtgcgcacgg cggcattgcg ctccagtgga
    43381 aaaacctggg tcttttgttg cagacgacga attgccggag agttgatgat acgcccgcga
    43441 tcgctctcga agatccgcag gatctcatgt tcggttttaa cgccctgcgg tgaacggtaa
    43501 cgacgatgcc agtttatttt ttttcggaaa tcaatctgtg ccatgtcctc tcccgcgtga
    43561 gaaatacgct tccccgtaag cgcatggtaa actatgcctt caaatcgggc ttatcgcgag
    43621 taaatctatg aaaatcggca tcattggtgc aatggaagaa gaagttacgc tgctgcgtga
    43681 caaaatcgaa aaccgtcaaa ctatcagtct cggcggttgc gaaatctata ccggccaact
    43741 gaatggaacc gaggttgcgc ttctgaaatc gggcatcggt aaagtcgctg cggcgctggg
    43801 tgccactttg ctgttggaac actgcaagcc agatgtgatt attaacaccg gttctgccgg
    43861 tggcctggca ccaacgctga aagtgggcga tatcgttgtc tcggacgaag cgcgttatca
    43921 cgacgcggat gtcacggcat ttggttatga atacggtcag ttaccaggct gtccggcagg
    43981 ctttaaagct gacgataaac tgatcgctgc cgctgaggcc tgcattgccg aactgaatct
    44041 taacgctgta cgtggcctga ttgttagcgg cgacgctttc atcaacggtt ctgttggtct
    44101 ggcgaaaatc cgccacaact tcccacaggc cattgctgta gagatggaag cgacggcaat
    44161 cgcccatgtc tgccacaatt tcaacgtccc gtttgttgtc gtacgcgcca tctccgacgt
    44221 ggccgatcaa cagtctcatc ttagcttcga tgagttcctg gctgttgccg ctaaacagtc
    44281 cagcctgatg gttgagtcac tggtgcagaa actggcacat ggctaagtca ctgttcaggg
    44341 cgctggtcgc cctgtctttt cttgcgccac tgtggctcaa cgccgcgccg cgcgtcatca
    44401 cgctttctcc cgccaacact gaacttgcct ttgccgccgg gatcacgccg gttggggtca
    44461 gcagctattc cgactatcct ccacaagcgc aaaagattga acaggtttcc acctggcagg
    44521 ggatgaatct ggaacgcatt gtcgcgctga aacccgatct ggtgattgcc tggcgtggag
    44581 gtaatgccga gcggcaggtt gaccagctgg cttcgctggg aataaaagtg atgtgggtcg
    44641 atgcgacaag cattgaacaa attgccaatg cgttacgtca actggctccc tggagtccgc
    44701 aaccggataa ggccgaacaa gccgcgcaat ccctgctgga tcaatacgcg caattgaaag
    44761 cgcaatatgc tgataaacct aaaaaacgtg tttttctgca attcggcatt aatccgccat
    44821 ttaccagtgg aaaagagtcg attcaaaacc aggtactcga agtttgtggc ggagaaaaca
    44881 tctttaaaga cagccgggtt ccctggccgc aagttagccg cgaacaggtg ttagcacgct
    44941 cgccacaggc gattgtcatt acaggcggac cggaccaaat tcctaaaatc aaacaatact
    45001 ggggtgaaca gctcaaaatt cccgttattc ctctcacgag tgactggttt gaacgtgcaa
    45061 gcccacgtat tatcctcgct gcacaacagc tctgtaatgc gctttcacag gtagattagc
    45121 gcctgacctt tttaggtgct cagtggggat tgaacaatgc tcgtctattg gctggatata
    45181 gtcggcacag cggtatttgc catctccggc gttttgttag ccggaaaatt gcgtatggac
    45241 ccttttggtg ttctggtact gggcgtggtt accgctgtag gcggcgggac aattcgcgac
    45301 atggcgctgg atcacggccc ggtattttgg gtgaaagatc ccaccgatct ggtcgttgca
    45361 atggtaacca gcatgctgac catcgtgctg gtgcgccagc caagacgctt accaaaatgg
    45421 atgttgccgg tgctggacgc cgttggtctg gcggtgtttg tcggcattgg cgtgaataaa
    45481 gcctttaatg cggaagccgg tccgttaatc gcggtttgta tgggcgtcat tactggcgtt
    45541 ggcggcggga tcattcgtga tgttctggcc cgcgaaatcc ccatgatttt acgtacagaa
    45601 atctacgcaa ctgcctgtat tatcggcggt attgtccacg ctacggctta ttacacattt
    45661 tccgtaccac tggaaacagc cagtatgatg ggcatggtcg tgacgctatt gattcggctg
    45721 gcggctattc gttggcatct taagctaccg acgtttgcgc tggatgagaa tgggcgttga
    45781 agagagaaga tgtgccggat agtttatccg gcacatgaac aacagattag atactaaagg
    45841 aagaaccgca accgcaggtg cttttcgcgt tcgggttggt cacgatgaaa cgagaacctt
    45901 ccagtccttc ggtatagtca acggaaccgc cgaccagata ttgcaggctc atcggatcaa
    45961 ccaccaggcc aacgccctgt ttttcgatgg tcatatcgcc ttcgttcacc tgatcatcaa
    46021 aggtgaaacc atactggaag ccgctgcaac cgccaccggt gatatacacg cgtaatttca
    46081 gattcgggtt atcttcgtca gcgatcaggc ttttaacttt gttggctgct gcgtcggtaa
    46141 actccagcgg cagtgctacg tcatcactca tattttgctc caaacgacat cggcaattgg
    46201 gcaaattcta acccaataat aatcgccatt atctaatacc ctggtatttc gttcaagtat
    46261 tctcgctggc tgatgcggcc ttgcttcgcg ccagttgctc agcttcctgt tttgccagcg
    46321 tgcgcgcaag aatcgccgag tatagcggtt tcccgccggt aaattgcgct aatagtgttg
    46381 cgccaagacc ggtaataatc attggcaaaa tgagctggta gttatcggtc atctccagaa
    46441 ccagaatgat ccccgttaac ggcgcgcgaa tagatgccgc cagtaacgcc cccattccgg
    46501 caatagcaaa cgtccccgcc tcaaggtgat attgcggaaa cagctcaacg gcaaccattc
    46561 cgaaagcggt tcccagcaca gtacccagcg ccagcatcgg ggcaaaaata ccgcccggcg
    46621 cgccggaaga gaagcagagt aaggtggtaa tgacccgcgc gacgaagata aacaccagca
    46681 ttcccatgct gaaattcccc gcggtagcga taggaatcag gttaaaaccg ccgcccgacg
    46741 ttgctggtgc cacaaacccc agcaatccac acagaccgcc aatcgcacca cccatcagta
    46801 cccatttggt aatattgccg ccgtgcacac ggtgcagcaa atcctgcatc cccagcaccc
    46861 atttattaaa aatagggccg aaaatgccaa aaataatacc gaggatcaga taaagccaca
    46921 gcgtattaag cggcgcgtca gaaagtttac cgacgtcaat caacgcaact tcatgattaa
    46981 aaatccggta cataatggtc gacataatga cgccaataaa taccgcttta atcgaaatta
    47041 acgtatagcg aaactgcgga cgcatctctt cgataataaa caaaataccc gccagcggcg
    47101 cgttaaaggc cgccgccaga cccgccgccg caccggttgc caacagcgta tggcgagctt
    47161 catcaccttt caggcggaaa acatcgagta ccatccgccc gatattaccg ccgatctgca
    47221 cggttggccc ttcgcgcccc aacaccatgc ctccgccgag tgtccccagc ccgccaaaga
    47281 atttcaccgg caatacacgc caccagcgaa caggacgttg atcttccagc gccccttcaa
    47341 tttccgggat ccccgacccc cctgcttcgg gcgcgtattt gcgcaccaaa aagtagccga
    47401 acatcgccag caccgccgaa cagagaaaag cgacggttaa cagtagcgga taattatcag
    47461 cagtatgtac cagcgccccc atacgttggt tctgcaacca ggcgacacct ttgtcaaaag
    47521 caaccgctgc cagcccgaca agcgtgccga cgactgccgc cataaacaaa atggctaacg
    47581 gggttttatc gcgctcaaga agttggcgaa tcagttgtct gcgtcgcagg cgcgcggcct
    47641 gcggtgtttc taaagaggga gtatcagttt tcatccaatg atcacttatt ggtcatacaa
    47701 ataagatgac ggcattttac tcgcacgttc catgaaaatc ctttgcaaag cgtaatgttt
    47761 caaatgcaca aaacttttat actctaagaa atcaggattg caccagtaca agcagcctga
    47821 tgtttgacga gtatttaact tgttatgaat aacatagaat agcagccatt cactttttct
    47881 acgaatcagg aaccctccat gagtaagtct gaaaatcttt acagcgcagc gcgcgagctg
    47941 atccctggcg gtgtgaactc ccctgttcgc gcctttactg gcgtgggcgg cactccactg
    48001 tttatcgaaa aagcggacgg cgcttatctg tacgatgttg atggcaaagc ctatatcgat
    48061 tatgtcggtt cctgggggcc gatggtgctg ggccataacc atccggcgat ccgcaatgcc
    48121 gtgattgaag ccgccgagcg tggtttaagc tttggtgcac cgaccgaaat ggaagtgaaa
    48181 atggcgcaac tggtgaccga actggtcccg accatggata tggtgcgcat ggtgaactcc
    48241 ggcactgaag cgaccatgag cgccatccgc ctggcccgtg gttttaccgg tcgcgacaaa
    48301 atcattaaat ttgaaggttg ttaccacggt cacgctgact gtctgctggt gaaagccggt
    48361 tctggcgcac tcacgttagg ccagccaaac tcgccgggcg ttccggcaga tttcgccaaa
    48421 cataccttaa cctgtactta taacgatctg gcttctgtac gcgccgcatt tgagcaatac
    48481 ccgcaagaga ttgcctgtat tatcgtcgag ccggtggcag gcaatatgaa ctgtgttccg
    48541 ccgctgccag agttcctgcc gggtctgcgc gcgctatgtg acgaatttgg cgcattgctg
    48601 atcatcgatg aagtaatgac cggcttccgc gtggcactgg ctggcgcaca ggattattac
    48661 ggcgtagtgc cagatttaac ctgcctcgga aaaatcatcg gcggtggaat gccggtaggc
    48721 gcattcggtg gtcgtcgtga tgtaatggat gcgctggctc cgactggccc ggtctatcag
    48781 gcgggtacgc tttccggtaa cccgattgcg atggcagcgg gtttcgcctg tctgaatgaa
    48841 gtcgcgcagc cgggcgttca cgaaacgctg gatgagctga caacacgtct ggcagaaggt
    48901 ctgctggaag cggcagaaga agccggaatt ccgctggtgg taaaccacgt tggcggcatg
    48961 ttcggtattt tctttaccga cgccgagtcc gtgacgtgct atcaggatgt gatggcctgt
    49021 gacgtcgagc gttttaagcg tttcttccat atgatgctgg atgaaggtgt ttacctggca
    49081 ccgtcagcgt ttgaagcggg ctttatgtct gtggcgcaca gcatggaaga tatcaataac
    49141 accatcgatg ctgcacgtcg ggtatttgcg aagttgtgat aattttgcct cgcccaccgg
    49201 gcgaggcatt ctattaacgg ctctgctttc tcagcaaata gataaaatat ggcgcgccga
    49261 taaaggtcga cagcagcccc gccgggatct ggaatggaaa cagcaccatc cgcccacacc
    49321 agtcagcgaa taccagcagt aaaccaccca ccagcgccga aattacgatg tgtggcatcg
    49381 tccgtcgaaa gcccatcatc cgcgcaatat gcggtgccat taaaccaaca aaactcaacg
    49441 gtccaatagt catcgtcgcg gtcgccgtca ggcaagccgc taacagcagc agcgcaattc
    49501 gcgtcggcgt cagcgccatt cctacggctc gtgcggtatc accacccagc ggtaaaatgg
    49561 tcagccagcg gcggtacagc ggggtaatcg ccagcaaaat caccatcaca attccggtgc
    49621 gccagacctg cgcatcggtg gcgttgtagg tcgaaccaga gatccaggtc agcacttgcg
    49681 ccattcgcgg gtcaccactc gcctgcaaca tcattaaaag catggtgaat gcggtactta
    49741 acgccatccc cgccagtaac atgcggtgtg gggaaaatcc accgcgcccg gcggcgatca
    49801 taataatcaa cagcgtcacc gccgcaccga tacttcccgc gggtaacagc cagccaaagg
    49861 cattacccgg caccagaaac agcattaaca ccacgccaaa cgccgcgcca gagctaatcc
    49921 ccagcacttc cgggcttgcc atcgggtttc cggtcagccg ctgaataata cagcccgcca
    49981 ccgccagcat gacgcccgca aacagcgccg ccataattcg cggccagcgc cagggcatca
    50041 aatcctcgag caacgccccg ctcgcccacg tccagccgtg cgcatcacga ccaaacgaca
    50101 gcgccaccac cacagccatc aacagcagca cgccgcccgc gagggcaaac gccagcacat
    50161 gttggcgttc agccgcgaca cgatcgttga ccttcatatc cggcgcgcta atgctgcgta
    50221 aacgcggcaa cagccacagt agcagcggcg caccgatcaa cgcagtgacc gaaccggtgg
    50281 acacttccat ccacacgcga gtcagccaga ggatgatttg atcggaaagc cagaggatca
    50341 gcgcaccaat cagcgacgcc agcatcaacc gtggcagcag acgccgcgcc cccagcattt
    50401 ttgccagcag cggcgcgaac aacccgataa agccgataat ccccacagcg ttcaccagca
    50461 gcgcactgat gacaatcgcc agcgacagcg ccgccagacg cgcgagcgac aaggccagcc
    50521 cgagattgcg cgccacgcca tcatcaagcc ccatcagggt taacggacga agtagcagca
    50581 acgtcagcat cacaccgccc agcaactgcg gccataaacg ctcaacaccg ccccagtcgg
    50641 tttgcgtcag cgttccggtg ctccacagga acatgctttg cagttggtca tggtggaaga
    50701 taaccagtaa ctgattgatt gccccacaat aaaggctcac taccagcccc gcgaggatca
    50761 acgttaccgg cgaaagccgt ttcccccacg cgacgccaaa gacgattaag ccaacaacgc
    50821 aagcgcccgc cagcgcagca aactgactcg ccatcgcccc tgggatcgcc cagagcgtag
    50881 tgacggtaat ccccagttgc gcgcctgtag caacgccaag tgtcgtcggt tccgccagcg
    50941 gattacgcag cacttgctga aacagcacgc ccaccagccc caggcctgcg cccaccagca
    51001 gcgaaatcgc cagacgcggc agcaagctgt agtgaaaaat catctgctcg atgacgtcaa
    51061 tatccggcga ccaggcagcc tgcgcccact ggctacgcgg cagcgcctgc gagaagttca
    51121 tccaggtcag cgccgtagcg acaatcacca gcagcgccag caataatgcc gggaaaagcg
    51181 caatacgttt actcacgctt tacctccaat ggcgttatcc agaacgcgca caaagtgcat
    51241 tgccgagagc gttgcaccat aaaaccagac tgcgggtacg cgctgaaagc gtccggcgcg
    51301 gacaaacggc atggcctgcc acagcggcgt tgccattagc gcatccatgt ctttgctgtt
    51361 gtcgtgatca aaacagagca catcaacgtc tttatacgct gccagacgat cgatgctgac
    51421 ggcggtactg ccccagaagt tggtttcccc ttgccaggca tttgggatgc cgtactcatc
    51481 aagaatttcc tggaacaagc tgtttggacc gaagaccagc atatggcgcg gatcgataag
    51541 cgtcgtcagc aataacggac gcgcaccacg cttcacaaag cggggtttca tgctgcggat
    51601 aaagtcttcg tattgcgcta aatgcgtttc ggctgcgctt tgcaggttaa gtaaatccgc
    51661 catttccgtc agcgatttac gcgccatcgc cagcggctgt ttgccatcgc tgaagttaaa
    51721 tccgcgcccc ggcgcaatgc gtgccagcat ttctggtgaa gggccatatc ctgccgacca
    51781 gaccataaac gacggtttca tttcggtcag cagttcaagg ttaggttctg tacgcaaacc
    51841 aacgtcaatc actgagtccg gcaatggcgg ttcgctgacc cacaggcgat agttgatggt
    51901 atccgccacg ccgtaaggca cgatgccgag cgccagcagt aattccaccg gcaaccactc
    51961 cagcgccact atacgattgg gatcaatagc cgccgcgtgg gcggtattca tctgccataa
    52021 caacggagaa agcgccatcg ccgttaacag tcgacggcgc gaaataagag gtaagccgct
    52081 catcaataaa caaaactcac aggtgcagca cccgccggat gcggcaaaat acccatcggg
    52141 atgccataaa tcatttcgag ggtttcgccg cgcataattt ccgcaggcgt tccctgagca
    52201 atcatttcac cgccgcgcag ggcgaccaga taatcacagt aacgcgcagc catattgata
    52261 tcgtgcagca cggcaatgac cgtcaggcca cgttcctgac ttaaacgatg caccagtgcc
    52321 agcacatcaa cctggtgagc gatatccagc gccgaggttg gctcgtcgag caacagacaa
    52381 cggctatcct gcgccaccag catggcgatc cacgcccgct gacgttcgcc gccagagagg
    52441 ctatcgacca gccgatgcgc cagcggtttt aagccaacca gcgagatagc ttcctcgact
    52501 ttttcgcgat ctgccgcccc aaagcgcccc agcgcgccat gccacgggta acgaccaatc
    52561 gccaccagtt cacgcacggt catcccttct gccggaggaa gctgctgcgg caaataagcc
    52621 actttgcggg cgaacgcttt gctgctccag ttttccagcg gctgggcatc aagaagaatc
    52681 tccccttctg acggaggctg atgacggcca agcattttga gcagagtgga tttaccagaa
    52741 ccgttgtgac caatcagagc ggtcactttc ccggcaggaa aggttaacga cagcggatgc
    52801 aaaagcgtgc gcccgggcac acgaaaggag atattacgca gtgcaaaagt ggtatcggaa
    52861 tgattcgtgt attcctgcat aacagccaac ttgtgaaatg ggcacggaaa tccgtgcccc
    52921 aaaagagaaa ttagaaacgg aaggttgcgg ttgcaacgac ctgacgttct gcgccccaga
    52981 agcagccata agtgttaaag cagctggcga cgtattcacg atcgaacagg ttgttaacat
    53041 gcagcgccac gttggagccc gccatgccga ctcgcgccag atcataacgc actaacgcat
    53101 ccacgaccgt ataacttccc actttaaagg agttagccgg atcaccatag ctggagccag
    53161 tataacgacc accggtgccc agcgtcagac ctgaaagcgg accgtcaaag aaggtatagt
    53221 cagcccacag cgaagccatg tgttttggca cctgtgccgg cgtattgcct ttataggtag
    53281 tatcggtggt gtattccgca tcggtgtaag tataagaacc gactacgtta acactcgccg
    53341 acagcgccgc tttcgcttcg atttctacgc cacgtgcgcg gatctcgcca ccttcaaccg
    53401 agaagaagga accctcaggg tccgccatca ggttgttggt tttagtgaga ttatacacgg
    53461 caccagtaac tacaatcgga cgatcttccg gtacatattt cacgccgact tcatactgct
    53521 tacctttaga cggtgcgaaa atattaccat ccttcccaac ttgcgaagaa ggttcaaacg
    53581 attcgctata gctgaagtaa ggtgttacgc cattatcaaa caggtagtta acaccaccac
    53641 gccaggtaaa ctgtttgtca tcgcgtttat cggtcgtcgc ggcaacgcgg ttaagagatt
    53701 cttgatctgc ccagtcgtaa cgaccgccca gggtgaccag cactttatcc cactgcgcct
    53761 gatcctgaac ataaacgccc gtttgtttct gcttattcag aatgcggtaa ggaccggagt
    53821 ttgccggatc tttcgcattg aagtcgaaat cggtattcac cggattgtac agatcgagca
    53881 gcggtacgga gtcgtcataa ccaaaccagg cgttgatgtc attacgcata cgcataaagt
    53941 cgacaccggt cagcagggtg tggtcgatat cgccagtggc aaacttgctc tgcaactggg
    54001 tatcaacaga gaagttttgc agcttctcat catcaacgac gtatttacgc gccagataat
    54061 ggcctttatc cgctggcgct aatgccgcac actgtttgct gtaagcattc gctggatcgg
    54121 agcagacgcc gtaaccataa acgctgtttt gcgaggtttt gttttcagca aagcgcaggt
    54181 tctgacgcac agtaaaggtg tcgttaaatt cgtgatcgaa gctgtagccc accatcttct
    54241 cattacgaga ataggtgttg ttcttcgccc cttcgttaaa gtctgtcggc agacgcttac
    54301 cgttcggcag cggctcaacg gttccctctt tcggcaacca gccgtaataa cccgtttccg
    54361 gctcgttctg gaagtaagag aggaaggtga aattggtttt atcatccgga cgccaggtga
    54421 acgccggtgc aatagcataa cgctgctctt ctgacccttt ctgctgggca ttggcagaac
    54481 gcgcaagacc ggtcaggcga taagagtaaa cgccgtcatc atccagcgca tcgctaaagt
    54541 caaaaccagt ctggaacagg ctgtcagtac cggctttaaa ctgaacttct ttcagcggtt
    54601 cggtggtcgg acgcttgctg accatattca acaggccgcc aggactgctt ttaccgtaaa
    54661 gcacggaaac cgggccacgc ataatttcag cgcgttccag catatacggg tcaatgaccg
    54721 catcgttata gaagttgccc tgcaacttca ggccattcag atagttattc tggctttggc
    54781 cttctgccgc aaaaccgcga atgatcaggt ggtcataggt gttggatgcg ccacgcgtac
    54841 caacagagac acccggcgtg tagctaagcg cttcttttac cgacttcggc tgatgcagcg
    54901 ccatctcttc ggcggtcaca acagaaatag actgtggcac tttttgaatc ggcgtatcgg
    54961 ttttagtgcc ggtagctgac tgtcgcgccg caatagttgc agcaggcccc catgcgcttt
    55021 cttgcggcgc aggtgcagcg gtaacggtga tagtgtcttc tttcggttca accgctgcct
    55081 gtgcataaac agacatgccg ctaaccgctg tggctactac aactgcgatt ttacgcagtg
    55141 agtgttttgg ctgagcagtt ttggaacgcg ccattggtat atctctgatg taaagtgaat
    55201 gataacgtaa acgagaatga ttattataaa gataagataa tattcgaaac gctggcgggt
    55261 tcgcaagcgg tacgcaatct gagcgttcct gaagggttaa taaacaacca gatgaaaaga
    55321 aagggttaat attttagatg ggatgttatt ttaccggatg gcaactcgcc atccggtatg
    55381 tcacgcttag acgttaatta ctaccaaaca tatccttgat ccaaccggct acaccgtcgc
    55441 tgtctttctg ctcttgctga gcaggttgct gttgcggctg ttgctgcggc tgtgaagact
    55501 gatcaaacgg attgcctgac ggctgctgct gcatctcact ctgctggcac agcgattgcg
    55561 gatcgctggt ccagaccggc aagacacgca tgccaccgct gcaaacaaag ttgccgtcgt
    55621 agtccacgcc catatctgca atatcttctg gcggaacaag attcagcggc gttggcgtct
    55681 ggttagccag ataacgctga taaatcgaca ttgccccgct ggcaccatac agtttggtcg
    55741 gctggttgtt atcacggccg acccaggtga tggtcaccgt gctgccgtca atgcccgcaa
    55801 accaggtatc tacgttattg ttggtagtcc ctgttttccc tgccagatgc aggttcgggt
    55861 atttcgcccc aagctgacga cccgtaccac gttgtaccac ctgctgcatg gtccatagtg
    55921 tcagatacgc cgcctgcgcc ggaacagcgc gttccgcctg cgggaagctc tgatacagca
    55981 ctttgccatc ttccgcgatt accgaacgca gcgcagaaag cggtgcacgg ttaccaccgc
    56041 tggcgatggt ctggaatgcc tgcgccactt cgattggcgt taagttcaac gcccccagca
    56101 gcatcgccgg aaccggatgc agctgatctt tcggcacgcc cagtttaatc caggtatccg
    56161 taaccgcagg cagccccagc gccatcccca gatttaccgt cggcacgttc atcgaacggg
    56221 tcaacgcatc caccagcatc actctgccgc tttcgctata acgacggtca tcgttctgcg
    56281 gtgaccagac ctggccattc ggctgacgta gcgcgattgg cgcatccgca atccacgtat
    56341 tcagacgata gattttcggc tggcttaaag ccgtcagata agtcgctggt tttgcaaggg
    56401 aaccaatcga acgccgcgcc tgcatcgcac ggttgtagcc cgcaaactgc ggctcagaac
    56461 caccgaccat cgcacgaact tcaccactaa agcggtcgac gaccacaatc gcggtttcca
    56521 gatcgctcag cttacgctgt ttcttaagtg ccggaatgcc ttccacggcg gctttttctg
    56581 ccgcgtcctg ggccaccgag tcaaaggtag tgaagatctt cacgccggag agatctttta
    56641 ccttatcgcc cagttttgcc tgcaactcct gacgcaccag ctgcataaag gctggctgag
    56701 gagagatcac cccaccacgc ggctgaacac ccagcggacg cgcactcaac atgtcataga
    56761 gttcttgatc aataatctgt tgctgttgca gcagacgcag taccagatta cgtcgctcca
    56821 gcgccagttt cgggttacgc cacgggttgt agatggacgc ccctttcacc ataccaacca
    56881 acagcgcctg ctgatcgagg ctcaattctt ctaccgggcg accaaagtaa tacaagctcg
    56941 ccagcgggaa gccgcggatt tcgttgtcgc cgctctgacc gagatacacc tcgttcatat
    57001 acagctcaag aatacggtct ttgctgtaac gcgcgtccat gatcagcgcc atgtaggctt
    57061 cattcgcttt acgccaataa gagcgctcgc tggagaggaa caggtttttc accagctgtt
    57121 gcgtcagcgt gctcgccccc tgcaccgtgc gacctgcggt caggtttgcc agcaccgcac
    57181 gtccgatgga gtagagactg attccatcat gctcgtagaa atggcggtct tctgtcgcca
    57241 gcaaagtatc caccagcaaa tccgggaaac cactgcgcgg cacaaacaaa cgctgctcac
    57301 cgtttggtga ggagatcatg gtgatcagac gcggatcaag acggaagaaa ccgaactgac
    57361 ggttgttctc catattgacg atggtcgcca gatgatcgcc atcaaaggtc agacgcgcgc
    57421 gcacctgtcc ttctttactg tccgggaaat caaacggacg gcgaatcatc tcaatgctgt
    57481 tggcctgcac ggtaaattcg ccaggacggg tcatttttga cacctgacga tactgggttg
    57541 cctccagcag cttcaccatt tcgttcttgc tgatggtcat gtctggctca aggttgacca
    57601 ttcggccata aactgccgca ggcagttgcc agaccttgcc atcaatacgg ctacgaattt
    57661 tttgatcgag ataaacgccg taaatggcga tcagcacggc aaaaacgata gccagtttta
    57721 acagcagcca tagccagccg cgtttgccac gaggcttacg ccctttgcct ttgcccttac
    57781 ctttgcgcgg catcggttct tcatcctcat agtcatcata atcgtcgtaa tcatcgtcat
    57841 cttcgtaacg acgacggctt accttttgtt tgaccggacg cgtcggtttc cctttgcgtc
    57901 caattggctc gcggtcattc ccggccatgc tttttctccg caatattcag gcgcaaaagc
    57961 ccgattttct gttcttctgt tacaagacag aagaagatat ctctcaaaat ttgagctcga
    58021 tcggtcccct ctcccttaag ggagagggtt agggtgaggg tgaacgtgcg cgctgatgcc
    58081 ctcaccccgg ccctctccca cagggagagg gagaaaacca accttacgaa tactttttcg
    58141 tccgtcgcgt cggtgccgtg tttgccgggt cgtccggcca gacgtgtttg ggataacgcc
    58201 ctttcatctc cttttgcacc tcacgatacg cccctttcca gaaggccccc aaatcacgcg
    58261 tgatttgcag cggcctttgg gcgggtgaaa gcaattccag caccagcggc acgcgcccct
    58321 gggcgatcgt cggattagtg gcctcaccaa acatctcctg cattctcacc gccagcgcgg
    58381 gcgggttatc ttcatgataa cgaatggcga tccggcttcc cgtcggcaca gtgtaatgcg
    58441 caggcaattc actatccaga cgttgctgca ttccccaatc aagtaatccg cgtagtgcct
    58501 gataaatgtc gagtgatttc aggccgcgta gtgaatgtac accggccata tgtggcaaca
    58561 gccacgtttc cagcgtcgcc aacaaacttt catcatcaac cgctggccag tcatattctg
    58621 gcaaccactt tccggcgcat aacaaacgca agcgtagctg ttccgcttcc gccgtccagt
    58681 tgagcacgct taaaccttta tcacggatgc cgttaagcat cgcctgatgc aactcgtctt
    58741 ctgacggttt cgccaacggc tgcactttca ccgtcaactg accgatttgc agccgacgcc
    58801 aggctttcag tgtaccttgc gcgtcatccc attccacggt gtcagattgc tgcaccagct
    58861 gcggacagcg tcgcactaat tcatcaatat cgaccggtag cgccagtaaa atccgcgcat
    58921 ccggcgaggc gctgccctgc aataataacg gtgcgatcaa ccattcgtgg cggcttagcg
    58981 cgtcgtcggc atcgagcatc gctcccatgc cgtttgccag ttgatagcgt ccatcttgcc
    59041 cacggcggcg agcaatgcga tcggcaaacg ccccggcaag tagcggcgcg ataagcgaac
    59101 tgtctgcctc accgccgcgt acgtttaagc gttttaacag ttgctgacta cgttgctgcc
    59161 aggctggttg attgcgcgaa aacgccacgc ccaggtcact attgcccatc cgtggcggct
    59221 cttcgagaat ggcggcaatt tttgccgcgg tagcagcttc gtcgtcgctc tttgcgctaa
    59281 ccagcattgc cgctaaacgc ggatcgttac ccagcgctgc cattttttgc ccttgcgcac
    59341 taagccgttc accgtccagc gcccccagca tccgtaacag gcgtttcgcg gccagtagat
    59401 tcaccgttgg cggttgatcc agccagctca tctgcgccgg atcgctgcat ccccattgca
    59461 gtaattccat cagcaaaccg gaaagatcgc tttgtaatat ttccggttca ctttgcgccg
    59521 cggcgcgttc tgcttgttcc ttggcgatta aatgcaggca gatacccggc tccagacgcc
    59581 cggcgcgccc ggcacgctgc gtcatggatg cctggctaac gcgttgagta attagtcgcg
    59641 taagccccgt gcgcggatca aaacgcgcca cacgctcctg ggcacaatcc accaccagac
    59701 gaataccttc aatggttaaa ctggtttcag caatattggt cgccagcacc actttgcgca
    59761 tcccttgcgg tgccgggagg atcgcttttc gctgatcgtt cagcgacaac gcgccataca
    59821 gcgggcagag caacacatca ctgccgatgc gcgaagccag ttgttcctgc acacgctgaa
    59881 tttctccgac gccaggtaaa aataacagta atgatccact ttcctgacgc agcatttcag
    59941 cggtggcaac cgcgacggct tcgtcaaaac gctgatgcgc gggcagcggt aaataacggc
    60001 gttcaaccgg aaacgagcgc ccttctgaga tgacgacggg cgcttctggc agcatttgct
    60061 gcaagcggtc gttatccagc gtagccgaca taatcagcag tttaaggtca tcacgcagac
    60121 cttgttgcac atcgagtaac agcgccaacg ccaaatccgc ctgcaagctg cgctcatgaa
    60181 attcatcaag gatcaccagt ccaacaccgc tcagttccgg gtcacgctgg atcatccgcg
    60241 tcagcacgcc ttcggtaacc acttccaggc gggtattcgg cccgacgcag ttttgcgcac
    60301 gcatccggta gccaacggta tcgcctggct tttcgttaag cagttccgcc agccgttgcg
    60361 cgacgttacg cgccgccaga cgacgcggct ccagcaggat aattttcccg ttaatgccgg
    60421 gatgcgccag cagttgcagc ggcagccagg ttgatttccc ggccccggtc ggcgcactta
    60481 ataacacctg cggcgcacaa tcgagagcgg taagtaattc aggtaagacg gcagcaacgg
    60541 gcaacgacga cacaaaacgc tccagagggt taacattctt cgcgccacat tgtagcatcg
    60601 cggtaattca taaccgagtg cctcacatgt ctgaaccgca acgtctgttc tttgctatcg
    60661 acttacctgc agaaatccgc gaacagatta tccactggcg cgccacacac ttcccacttg
    60721 aggcgggacg tccggtcgcc gccgataatt tgcatctgac tctggcattt ttaggcgaag
    60781 tgagcgccga gaaagagaag gcgctttctc ttttagccgg acggattcgt caacctggtt
    60841 tcacactcac gcttgatgac gccggacaat ggctgcgttc gcgtgtggtg tggttaggga
    60901 tgcgtcagcc tccacgcggc ttaatccagc tggcgaatat gctccgttca caggctgccc
    60961 gcagcggttg ttttcaaagc aatcgtccgt ttcatccaca tattacctta ttgcgcgacg
    61021 ccagcgaggc ggtgacaatc ccgccgccag gttttaactg gtcgtatgcg gtgacggagt
    61081 tcacccttta cgcctcctcg tttgcccgtg gacgcacacg ctacacgccg ctaaaacgct
    61141 gggcgctaac gcaataacaa ggattgtcgc aatggaattt tctccccctc tacagcgcgc
    61201 gacgctaatt cagcgttaca aacgtttttt agccgatgtg atcacacccg atggtcgcga
    61261 attaacgcta cactgcccga atacgggtgc gatgaccggt tgtgcaacgc ctggcgatac
    61321 cgtctggtat tcgacttcag acaacactaa acggaaatac cctcacacct gggaattaac
    61381 tcaaagcaag agcggcgcat ttatttgcgt caacacgctt tgggctaaca ggttgacgaa
    61441 agaggctatc cttaatgaat caatttcaga actgtcaggc tatagctcgc tgaaaagcga
    61501 agtaaaatac ggcgcagaac gcagccgtat tgactttatg ttgcaggcgg attcgcgtcc
    61561 agactgctat attgaagtga aatcggttac gttagcggag aacgaacagg gatattttcc
    61621 cgatgcggtc actgaacgag gtcagaaaca ccttcgggag ttgatgagcg tagcggctga
    61681 aggccagcgt gcggttatct ttttcgccgt gctgcattca gccattacac ggttttcacc
    61741 cgcgcgccac atcgatgaga aatacgcgca actattgtca gaagctcaac agaggggggt
    61801 agaaattctg gcttacaaag cggaaatttc tgctgaaggc atggctctta aaaaatcact
    61861 gccggttaca ttgtagtaaa gtaagtaact ggttaattta cattctggtc gcgtgcgcaa
    61921 atacgctttt cctcacacag ttgtcaagtg ttacgtttag ataattgcta tccggaaaag
    61981 catctgctat ttatagcggc ctcatttttc ccccgaacat ggggatcgat agtgcgtgtt
    62041 aaggagaagc aacatgcaag aagggcaaaa ccgtaaaaca tcgtccctga gtattctcgc
    62101 catcgctggg gtggaaccat atcaggagaa gccgggcgaa gagtatatga atgaagccca
    62161 gctggcgcac ttccgtcgta ttctggaagc atggcgtaat caactcaggg atgaagtcga
    62221 tcgcaccgtt acacatatgc aggatgaagc agccaacttc ccggacccgg tagaccgtgc
    62281 agcccaggaa gaagagttca gcctcgaact gcgtaaccgc gatcgcgagc gtaagctgat
    62341 caaaaagatc gagaagacgc tgaaaaaagt ggaagacgaa gatttcggct actgtgaatc
    62401 ctgcggtgtt gaaattggta ttcgccgtct ggaagcgcgc ccgacagccg atctgtgcat
    62461 cgactgcaaa acgctggctg aaattcgcga aaaacagatg gctggctaat tacagccgtt
    62521 ccatcacgtt taccacaggc gggaaatgct cccgccttat tttttgttca aagagatgac
    62581 agacacacac tatattggcc gcttcgcccc ctctccttcc ggcgagcttc attttggttc
    62641 tctgattgct gcgcttggca gctatttgca ggctcgcgcc cggcaaggtc gctggctggt
    62701 acgcatagaa gatatcgacc cgcctcgtga agttcccggt gccgcagaaa ctatcctgcg
    62761 ccagctggaa cattacggcc tgcactggga cggcgatgtt ctctggcaat cgcaacgtca
    62821 ccacgcctat cgcgaagcac tcgcctggtt acatgagcaa ggactaagtt attactgcac
    62881 ctgcacgcgt gcacgtattc aaagcattgg cggtatttac gacggtcatt gccgggaatt
    62941 gcatcatgga ccaggcaacg ccgcagtgcg tatccgccag cagcatccgg tcacccagtt
    63001 tactgacctg ctgcgaggca ttattcacgc cgacgaaaaa ctggcacggg aagattttat
    63061 tattcatcgc cgtgatggtt tgtttgccta taacctggcg gtggtggtgg atgatcactt
    63121 ccagggcgtg agcgaaattg tccgcggggc ggatttgatc gaaccgacag tgcggcaaat
    63181 ctcgctgtac cagctttttg gctggaaagt gccagattac attcatctgc cgctggcgct
    63241 taatccacaa ggcgctaaac tttccaagca aaatcatgcg cctgcgttgc cgaaaggcga
    63301 tccacgcccg gtactaatcg cggcacttca atttctgggt cagcaggtag aagcacactg
    63361 gcaggatttc agcgtcgaac aaatccttca gtcagccgtc aaaaactggc ggcttaccgc
    63421 agtgcctgag tcggcaattg taaattcaac attctcaaat gcgtcatgct gagctatgat
    63481 tagccgctat ttttttgtcc tgaatgatgt ttgacactac cgaggtgtac tatttttacc
    63541 cgagtcgcta atttttgccg caaggtgcta agccgcgagg aaagcgaggc tgaacaggca
    63601 gtcgcccgtc cacaggtgac ggtgatcccg cgtgagcagc atgctatttc ccgcaaagat
    63661 atcagtgaaa atgccctgaa ggtaatgtac aggctcaata aagcgggata cgaagcctgg
    63721 ctggttggcg gcggcgtgcg cgacctgtta cttggcaaaa agccgaaaga ctttgacgta
    63781 accactaacg ccacgcctga gcaggtgcgc aaactgttcc gtaactgccg cctggtgggt
    63841 cgccgtttcc gtctggctca tgtgatgttt ggcccagaga ttatcgaagt tgcgaccttc
    63901 cgtggacacc acgaaggtaa cgtcagcgac cgcacgacct cccaacgcgg gcaaaacggc
    63961 atgttgctgc gcgacaacat tttcggctcc atcgaagaag acgcccagcg ccgcgatttc
    64021 actatcaaca gcctgtatta cagcgtagcg gattttaccg tccgtgatta cgttggcggt
    64081 atgaaggatc tgaaggacgg tgttatccgt ctgattggta acccggaaac gcgctaccgt
    64141 gaagatccgg tacgtatgct gcgcgcagta cgttttgccg ccaaattggg tatgcgcatc
    64201 agcccggaaa ccgcagaacc gatccctcgc ctcgctaccc tgctgaacga tatcccaccg
    64261 gcacgcctgt ttgaagaatc gcttaaactg ctacaagcgg gctacggtta cgaaacctat
    64321 aagctgttgt gtgaatatca tctgttccag ccgctgttcc cgaccattac ccgctacttc
    64381 acggaaaatg gcgacagccc gatggagcgg atcattgaac aggtgctgaa gaataccgat
    64441 acgcgtatcc ataacgatat gcgcgtgaac ccggcgttcc tgtttgccgc catgttctgg
    64501 tacccactgc tggagacggc acagaagatc gcccaggaaa gcggcctgac ctatcacgac
    64561 gctttcgcgc tggcgatgaa cgacgtgctg gacgaagcct gccgttcact ggcaatcccg
    64621 aaacgtctga cgacgttaac ccgcgatatc tggcagttgc agttgcgtat gtcccgacgt
    64681 cagggtaaac gtgcatggaa actgctggag catcctaagt tccgtgcggc ttatgacctg
    64741 ttggccttgc gagctgaagt tgagcgtaac gctgaactgc agcgtctggt gaaatggtgg
    64801 ggtgagttcc aggtttccgc gccaccagac caaaaaggga tgctcaacga gctggatgaa
    64861 gaaccgtcac cgcgtcgtcg tactcgtcgt ccacgcaaac gcgcaccacg tcgtgagggt
    64921 accgcatgac agtggcgtat attgccatag gcagcaatct ggcctctccg ctggagcagg
    64981 tcaatgctgc cctgaaaaca ttaggcgata tccctgaaag ccacattctt accgtttctt
    65041 cgttttaccg caccccaccg ctggggccgc aagatcaacc cgattactta aacgcagccg
    65101 tggcgctgga aacctctctt gcacctgaag agctactcaa tcacacacag cgtattgaat
    65161 tgcagcaagg tcgcgtccgt aaagctgaac gctggggacc gcgcacgctg gatctcgaca
    65221 tcatgctgtt tggtaatgaa gtgataaata ctgaacgact gaccgtaccg cactacgata
    65281 tgaaaaatcg tggatttatg ctgtggccgc tgtttgaaat cgcgccggag ttggtgtttc
    65341 ctgatgggga aacattacgt gaaatactca cttcactagc agtaccacaa cctgccatct
    65401 ggtaatagct gtattatttt attttcttga cgagagaaat acaaaatgat acggctattt
    65461 cattcaccaa gaaaaaatta ttttcttatc ttactcctca catcccccct tacacattaa
    65521 atatacttaa tttacatttt agtttttttt aattcattca tgaaatagca cttcatgcaa
    65581 atagattagg cattttttgt ttaaacgacc taaaataaca acaattcaat acgccacata
    65641 aatatattca cacccgtgat aattcacccc tattacatcc tccaaaaact ttgagtaata
    65701 tccgctcgca atattaattt catataattt ttgtaaggat gcatgtaatg aaaaaagcac
    65761 ttctcgcagc cgctctggtt atggcttctg gttccgccct ggctgtagat ggtggtcata
    65821 tcgactttaa cggtatggta cagtccggta cctgtaaagt gggtgtggta gatactggta
    65881 tgcacagcgt taccactgat ggcgtggtta ccctggatac tgcgaatgtt actgatactt
    65941 ttgctgaagt tagcgcaact gctgtcggtt tactgccgaa agagttcatg atttctgttg
    66001 agtgtgatcc aggtgctccg aagaatgctg agttaactat gggttctgca agctacgcga
    66061 acaccagcgg taccctgaat aacaatatga acatcactgt taacggtatt gcaccggctc
    66121 agaacgtaaa cattgcagtt cataacatga aaaacaaagc tggcgctgct gaaattaagc
    66181 aggtccatat gaacaactct tctgaagttc aggaactgac attagacgca gaaggtaaag
    66241 gccagtacgt atttaacgca tcttacgtta aagcaccgaa cagcccggct gtaactgctg
    66301 gtcatgtaac cactaacgcg ctgtacaccg ttgcttataa gtaattgatg gtgtgttgag
    66361 cataaggttt gccagcaggc aaaccttatt ttcgcgaatt taatctttct gcagacagca
    66421 aggagtgctt atgtttccct tggttaaaaa aacggttagt gcattatttg tgagtactct
    66481 gttagccagt gcaccagcat tcgctgacat tattatttcc ggaacacgta tcatttataa
    66541 cgctgataaa aaagatgtta acgttcgtct ggaaaataaa gggaatcgcc ccttactgat
    66601 tcagaactgg ttggatactg gcgacgacaa tgccgatccc agccagatca aagtgccgtt
    66661 taccgcgacc ccaccagttt cacgtgtgga gccaaaacgc ggtcaaaccg taaaagtgat
    66721 gtataccggt gcaacagccc tgccgaaaga ccgtgaaagc gtctactggt ttaacgtgct
    66781 ggaagtgccg cctaagccga aagatgctga ggctgacaaa aacttgttac agctggcttt
    66841 ccgcacacgc atcaaactct tttatcgccc gagtgggtta cagggagaac cggctgaagc
    66901 accggcgaaa atcacctgga aattaaacaa cgctcagtta caggcaaata acccaacgcc
    66961 atattacgtg tcctttaatg aggtcaagct ggaaagcggc ggcaaaacct acaacgtcaa
    67021 ttccagcatg gtcgcgccgt tcagccaggc aagctttggc gtaaccggtc tgccaggaag
    67081 cgtgtcatcc ggaaaagtgg tttttaaagc gatcaacgac tttggcggca acattgatgg
    67141 ttccgcaaca ttctaaaaaa taaacggatt ctttttaaca tcgccaggaa tgcaaccttg
    67201 taccagttta ctcatcaaaa aagccgtatc ccgaaaaaaa cgctacttgc ggcctgttgc
    67261 gccctttttt acagcggcaa cagttctgcg gcagacacgg tggaatatga cagttccttc
    67321 ttaatgggaa ctggcgcatc aacgattgat gttaaacgtt atgctcaagg caacccgaca
    67381 ccgccgggtc tctataatgt ccgcgtgttt gtaaacggtc aggcgacgtc cagcttagaa
    67441 attccgtttg tggatattgg cgaaaacagc gcagcggcct gtattaccca caaaaacctg
    67501 gcacaacttc acattaagca acctgaacag cctgtcactt tactcgccag agaaggtgag
    67561 gaagaggatt gtctggattt ggcaaagtca tacgaaaagg cggatgtgcg ctttgacggt
    67621 agtgaccagt ttctcgatct gacgatccct caggcctatg ttttgaaaag ctatggcggc
    67681 tacgttgacc cttctttatg ggaatcggga attaacgctg ccacactggc atataccctg
    67741 aacgcgtatc acacaagttc agataacgac aatagtgaca gcgtctatgg cgcatttaac
    67801 tcgggtatca atttaggagc ctggcacttc cgtgcgcgcg gtaactataa ctggacaaca
    67861 gataacggca gcgatttcga tttccaggat cgttacttac agcgtgacat tccggcaatc
    67921 cgttcacaga taattatggg tgacgcctac accaccggtg aaacgtttga ctctgtcaac
    67981 gtccgtggtg ttcgcctgta cagcgatagc cgtatgctac cttcggcgct ggccagttac
    68041 gctccgacca tccgcggtgt agcaaactcc aacgccaaag tcacggtgac gcaaagcggt
    68101 tataaaatct atgaaaccac cgtaccaccc ggtgaatttg ttatagacga cattagccct
    68161 tccggctttg gtagcgaact ggtcgtaacc attgaagaag cggatggttc caaacgcacc
    68221 tttacgcaac ccttctcgtc ggttgtgcaa atgcaacgtc ctggtgtagg tcgttgggat
    68281 ttcagtgccg gtaaagtcat tgatgatagc ctgcgttccg aacccaatat ggggcaagcc
    68341 tcttattact atggtctgaa taacctcttc acgggttata ccggcattca gttcaccgat
    68401 aacaactatc ttgccgggct gttaggtgtg ggtatcaaca ccagcatcgg cgcctttgcg
    68461 gtagacgtta cccattcccg tgctgaaatt ccggatgata aaacctacca ggggcaaagt
    68521 tatcgcgtga cctggaacaa acttttccag gataccggga catcatttaa cctcgcggcg
    68581 taccgctatt ccacccagga ttacctgggc ctgcatgatg cgttagtcct cattgacgac
    68641 gccaaacatt tgtctgccga tgaagacaaa aacaccatgc agacgtactc acgtatgaaa
    68701 aaccagttta ccgtcagcat caaccagccg ctgaaaacga cttacgaaga ttacggttca
    68761 ctgtttattt ccggtagctg gacggattac tgggcggcga acaatagccg cactgaatat
    68821 aatgttggtt acagtaaaag cgtttcgtgg ggcagtttca gcgtcaacct gcaacgtagc
    68881 tggaatgaag acggcgagaa agatgacgcg atgtacgtca gcgttagcgt acctattgag
    68941 aatattttag gtggcaagcg caaatcttct ggtttccgca atttaaatac tcagctcaat
    69001 accgatttcg atggttcaca tcagttgaat gttaacagtt ccggtaacac tgaaaacaat
    69061 ctggtgaact acagtgtcaa cgctggttat agcctcgata aaaacgccgg cgatttagcc
    69121 tctgttggtg gttatctcaa ctatgaatca gggttaggcg gtatttccgc ttcggcctcg
    69181 gccacttctg ataatagcca gcagtactcc atctccaccg atggcggctt tgtattacac
    69241 agtggtggtt taacgttcac taacaacagt ttcagcagta acgacacgct ggtgttaatc
    69301 aacgccccag gtgctaaagg cgcacgaatc aataacagta ataacgaaat cgatcgctgg
    69361 ggatatgccg tgacgtcttc tgtcagcccc taccgtgaaa accgggtagg tctgaacatt
    69421 gaaacactgg aaaacgatgt tgaactgaaa agtaccagcg ccaccaccgt accacgcagc
    69481 ggctccgttg ttttgacccg tttcgaaact gacgaggggc gttctgccgt gctgaatatt
    69541 actgccgcca atggcaaatc tattccgttt gctgcggagg tttaccaggg tgaggtgatg
    69601 atcggcagca tgggccaggg tggtcaggca tttgtgcgcg gtattaacga cagcggggaa
    69661 ttaacagtgc gttggtatga aaacaaccaa accattgact gtaagttgca ctaccagttc
    69721 ccggcccagc cacaaacgct gggaagtact aacaccttat tacttaacaa tcttacctgt
    69781 caggtagcaa atcactaata tggaaaattc tatgaaaagg atactactaa catccgcgtt
    69841 aataggtctg ggtttaccgg cggttggctc tgcaacagat cttaatgtcg attttacggc
    69901 caccgtatta gctaccactt gtacgataac catcgtacaa gatggtggcc cagttgtgac
    69961 gggtagcaac ggtgaatata gcctgacaat tcccgatgtt ggcctggata aagttgtcac
    70021 tggagatgtt gcagctcaag ccaactttaa attagtggct tccgattgca gcaatggtta
    70081 ttcaaagatt tttaccacac ttacaggaac aacggtttcc ggtaagttaa ttgttaatga
    70141 aacaacctct ggcgctacgg gtgtgggtat ggggattaag cgtagagata ccgcagactc
    70201 ggctttcttt acgccaaata atactgacaa atttgaatgg agtaccgatg agaaaactaa
    70261 cggtgtgcct ctgaccgtag cactgcgcga aacaacggcg ggagcaggac gcaccggtgc
    70321 gtttcaggca aaagccacct ttaacttcac ctacgaataa cagggacagc caccatgaaa
    70381 ttaaaagtca tcgctacact gattgctact gttgccgtgg gtgtcagcgt tagcaacaat
    70441 ctcacttatg cgagtacgac atccgcctct ttaaccgtaa acagtaacct gactatgggt
    70501 acctgtagtg cgaaggtagt ggataccagt ggtgccgtga tcgacaccgt tgtcttgggc
    70561 aatgtttata tttctgaact ggtagccaaa agcaaggtgc aaccatttaa aatccgcttc
    70621 agcgattgtg ccgggcttcc aggaaagagt gctcaactcg tcttagcgcc caaaaacaac
    70681 ggttgcgcag gaggcatgtc tgacactgcc gccttttcaa ataaactcaa caccaccacc
    70741 gatggtggtt caaacagagc agcagtcgaa gtatggacga ctacaagccc cgaaggaact
    70801 ggcagtgagc aattcaattg ttatcagcga aacgtcatca atgtcgatct ttctaatgcc
    70861 agcacaacac agccttatga tttcgattta agcgcgcgaa tgattatcgc tgacgggtgg
    70921 acagtcaatg acgtattacc gggtagattc ctctctccta cgactttcac aatcacttat
    70981 cagtaataac ggataaggat attagaatgc tatgcaggca tcataaattt gtacgttttt
    71041 tggggctggc aacagccctc attgcgcctt ttgcctatgc tggccaggat gtggatctca
    71101 cggcgcagat tgtggccagc acctgtcagg ttgaagtcag taataatggc gttgtcgatc
    71161 tcggcacagt gacgctggac tattttgccg acaatgtaac cccgacaacg gattatgcgg
    71221 gcggcaaaac gtttaatgtg aatgtggtct cttgcgataa cattcaaacg acacagtcgc
    71281 aaatgaaatt ggattttcag ccgcaggcgg gatcgcttgc acaggtgaat aatcaaattt
    71341 ttagtaatga atacgaacag caggctaccg gggcgaaaaa cgtcggcatt gtgatatttt
    71401 cggcccaacc gaatcagcag acatttaacg tgcgaggaac ggatggttcc tcgacagcaa
    71461 tttattcggt cgcgcctggc aatgctgttc cctcaacctg gaccttttat tcccgtatgc
    71521 agcgggtgaa taatgccctt cctcctgaat cgggaatggt aagaagccag gttattgtta
    71581 acgtatctta cgaataataa ggataattat gcaaaggaaa ggcaataaac tgttgattca
    71641 gttatgcagt gcgatactgc tattttttac cacatcctgg tatgcattgg cgaatgaatg
    71701 ttatatagag agaaatgctg aagggcctta tgatatggtg ataaacccaa agcccttcag
    71761 tatacagtca gcgatggtca cagtcccgga agaaatatcc ttcgcgacct gggattctca
    71821 tattgatctt cggggcgata agattggttg taaatcttta ggtagtaaca caagctcggt
    71881 gcattttctt aatactgctg atgccagcct cttatcaacc tatactacca accatggcaa
    71941 tgctttattg aaaacaaccg ttcccggtat tgtttattca gtagaattag tatgtacaag
    72001 ttgtggagcc gcggaggagc ttgatttgta tcttcctgca caaagtggtg cagataatca
    72061 tacagataac gccagctcaa agtgggccta tgagtatagt gacgatagct ggtatttacg
    72121 atttcgtttt tactggaccc cggaatttaa acctcaaaat ggggtttcag aaggaactgc
    72181 gatgccaggt aaaatagctt cctggtatat aggcaataat gaccaaccat ggataaattt
    72241 tttcgttgat acaagctccg ttcattttta cgttgacgaa ccgacctgtg caacagttgc
    72301 cctcgcacaa gatcagggca acgtcagtgg caaccaggta acgcttggga acagctatgt
    72361 ttcggaagtg aaaaatgggc ttacgcggga aatccctttt tctatccgtg ctgaatactg
    72421 ctatgccagt aaaattacgg ttaagttgaa agcggcaaat aaacccagcg acgccacact
    72481 agtaggtaaa acgactggct ctgcttcagg cgtggctgta aaagtaaatt caacttatga
    72541 caatagcaaa gtattgttaa aagcagatgg tagcaacacg gttgactata acttcgccgt
    72601 ctggtcaaac aacctgctgc ttttaccttt tacggcgcag ctggtaccgg atggcagtgg
    72661 tgctgctgtc ggtgttggaa cattttcagg taacgcgacc ttctccttta cctacgaata
    72721 agtaaaacgt cattccctca tcatcgcacc agagttctgc tctggtgcga acgttttccc
    72781 cacactaaaa accattgccc ccctgatttc gactgttaga atgcgatcaa tctgacttta
    72841 gctatcagga tacgttatga aaccaaccac cattgcttca ttgcagaaat gtaagcagga
    72901 aaaaaaacgc ttcgccacca tcaccgccta cgactacagc ttcgccaaac tgtttgctga
    72961 cgaagggctg aacgtgatgc tggtgggtga ttcgctgggc atgacggttc aggggcacga
    73021 ctccaccctg ccagttaccg ttgctgatat cgcctaccac actaccgcag tacgtcgtgg
    73081 cgcgccaaac tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
    73141 agcctttgaa aacgccgcaa cggttatgcg tgccggtgct aacatggtca aaattgaagg
    73201 cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg
    73261 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
    73321 cggcgatgaa gcgagcgatc gactgcttag cgatgcatta gccttagaag ccgctggtgc
    73381 acagttgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcact
    73441 ggtgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
    73501 gcacgatgcg ttcggcatta ccggcggcca tatccctaaa tttgccaaaa atttcctcgc
    73561 tgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
    73621 ttatccgggc gaagaacaca gtttccatta aggagtgacg ttgtgttaat tatcgaaacc
    73681 ctgccactgc tgcgccagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
    73741 gtacctacca tgggcaacct gcatgatggc cacatgaagc tggtcgacga agccaaagcc
    73801 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggag
    73861 gacctggctc gttacccacg gaccttgcag gaagactgcg aaaagctgaa caagcgcaaa
    73921 gtggatctgg ttttcgcgcc ctcggtaaaa gagatctacc cgaacggtac tgaaacccat
    73981 acttacgttg acgttccagg gctttcgacc atgctggaag gcgccagccg tccggggcat
    74041 tttcgcggcg tgtcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc
    74101 tgcttcggtg aaaaggactt tcagcaactg gcgctgatcc gcaaaatggt tgctgatatg
    74161 ggctttgata ttgagattgt cggtgtgcca attatgcgcg ccaaagacgg tctggcactg
    74221 agttcccgta acggttatct gacggcagaa caacgcaaaa ttgcgcccgg tctgtacaaa
    74281 gttttaagtt cgattgccga caaattgcag gctggcgaac gggatctcga tgaaattatt
    74341 gctattgccg ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc
    74401 gatgccgaca cgttgcagga ggtctctgaa aacagcaaac gggccgtaat tctggtagcc
    74461 gcctggcttg gcgatgctcg cctgatcgac aacaagatgg tcgaactggc gtaatactta
    74521 actggcgcta cggctgatgg cgccagttat taatttgccc tacgtcatgg cgatgacgtc
    74581 tgtaagtgat tattcatatc gctggcaatt tagttagagt taattagctt atcaatctct
    74641 gtaagaggta aagttgtcac ctctgcaacg tcttcccgag acatcccttt acttagaaaa
    74701 cgctgggcga attcctgtct taccttctgg agttcctctt gatatccctt ctgaaatccc
    74761 ttctcttcaa accactgcgc caacgtctgc ctggtttctc tccgtgtctt tatttccata
    74821 ggttccctta attatctggt cagttaacaa taacaattat ctggctataa ctgatgatgc
    74881 agacaactta gttaatcagc ttatcaatct cggcaagggg taaatttgcc atctctgcaa
    74941 cgtcttcccg agacattcct ttattcagaa gacgcaggac gaattcttgc ctttcctcct
    75001 gtcttcccct ctcttcaaac cactgcgcca gcgtcatcat agactcccct cccgtttccc
    75061 tgtctctcag cacaccataa aacaaatccg cttgttcagt atgaccgcgt tgcagcatat
    75121 agttttgcat ggcaactaac tgacttccgc tagtgtaccc ttcgtcaatc agcgtgacca
    75181 gttgctccag caataacatt aagtcgcgct ggcgaatatg cttttgcagt agttcgagaa
    75241 tcgcaatccg ccggtgctgc atgatttcgt catccggtgt gatagtgata tccaccagcg
    75301 gaaaaggact gttatagacg cgtcgcgcca gctccggcga gtaaaacata tcaaaccagc
    75361 acattgagag cggataaggt gtggtctcgc cctgataaaa cagtatcggc accaccagcg
    75421 gcagcttatc gtgatccgcc tccagatgcc ggtgcatggc agcaatagaa taacgcatca
    75481 tgcgaaaggc cattttctta tccggcttgc tttggtgttc aatcacaaca tgcagataac
    75541 cgggattgcc ctgcatttgc acggaataga gcacgtccgt gctgtgtcct ttcaggctct
    75601 cttcaatgaa actccccgac tctaaatgaa gcgtgttgag gtcacaaagt tcgcgtaatt
    75661 ccactggcaa atgtatctcc agaaagtcgc gagccgtctc cgcatgcatt aaaaattgtt
    75721 taaataccgc gtcatgcggt gtagtactcg gtgcatccat cttccctgtc ttccgtgttg
    75781 tcttgcgtga aggcagtatc cagtcaaggc gtgctatcgt cagccacctc tttattgttt
    75841 accgagcagc gttcagagaa atatcaattc gttacaggcg atacatggca cgcttcggcg
    75901 cggtacgcaa actgctgatg caaatgccgt cagatgagta gacactaaac aaaaatcggg
    75961 caatactgcg tgagaatttc ctcaaagcag gcaccgcctg cttcgttaac gacagggtag
    76021 aaaggtagaa gttatgattc gcacgatgct gcagggcaaa ctccaccgcg tgaaagtgac
    76081 tcatgcggac ctgcactatg aaggttcttg cgccattgac caggattttc ttgacgcagc
    76141 cggtattctc gaaaacgaag ccattgatat ctggaatgtc accaacggca agcgtttctc
    76201 cacttatgcc atcgcggcag aacgcggttc gagaattatt tctgttaacg gtgcggcggc
    76261 ccactgcgcc agtgtcggcg atattgtcat catcgccagc ttcgttacca tgccagatga
    76321 agaagctcgc acctggcgac ccaacgtcgc ctattttgaa ggcgacaatg aaatgaaacg
    76381 taccgcgaaa gcgattccgg tacaggttgc ttgattgtta tccctgcggc tggttactca
    76441 ccagccgcga catcgtctcc agcgaatccg ttcttaagat ataaagtcgt tttagtaaca
    76501 acggattatc ccccggtttt actttgcctt tcatggttgt caccgccagg tgaaatccgg
    76561 catcgtttgc tgccttcacg gctttgtcat taaagccgcc aaacggatac gaaagatacc
    76621 agacatgcgg attaaattgc gccagagcgc ggcgtgaacg tgcaaaatca aacagaatat
    76681 tgtgctcact acggctcagt aatatgggtc ggcgataacc atctacccga tgcaaaaaat
    76741 gggtatgtga ctggaaatca aatacatcgc gaatttcgtt aagctcagaa acgctcataa
    76801 attgcagcga ttttgggttc cacttctgcg ggtgacgttt gatgcgtgag gtaacaataa
    76861 acgccgtcgc cttcatgcca tattgtttca acacaggata cgcatagcgg ctcaccgact
    76921 tgaggccatc atcaaaggta atcaccaccg ctcgcgcagg gagattgatc ttattcttca
    76981 cgtaaccttc cagctgcacc atgctcagtg tcgcgtatcc cctgtcgcgc agccaggcca
    77041 tctggttatt gaaagcgcgt accgaggtgg tcgtcgaagt atggcgaaaa cgggtgtttt
    77101 cttcgtcgcg cagaatatgg tgataagtta gcaccggaag gccattatcg ggttgggcat
    77161 ccagcgcgct gatataggcc agtcgatcgc caatacggac ctgataccag gtttgattta
    77221 acctgtcttt cagtttatgc aaaatcgggt agcgcaaatt gtccgccagt accccaaatg
    77281 gcgcactgcc cgcactcggc gcgttataga ccggcgtatc tttccaggta actaagttct
    77341 gattactcag cggcttgttg aggtcaccca aaccgtcttc aactttttgt cgcccctgaa
    77401 ccggctcaag atgaccttta tcgataaacc ctttaccaaa gccaaaatta aatgcgtaat
    77461 aacttgcggc agtgggctcc acggcaatga tttgtccggc ccgaatattc cccacggtca
    77521 ccatcttgtc accaatttgc gcccagaccg cagcattttc gatggtttgc atataacggg
    77581 caggtaacgc ggcactgaca cttgcggtaa acagcatcag caggagaata acagcttgtt
    77641 tgtacatgaa aaaataaccg agggtgaaac aacgctttta ttgtagcaaa agcgctataa
    77701 gaatcgtggg ttaatactca tacaaattat gtttgaggac gaaaggcgga tttttttgtt
    77761 gttggtgcca aagactactc acctccggcg cacccagttc gacaatacgc tcgcgaaact
    77821 ctgaactggt catggtttca cggcaagcca tcatctgttc aattaacggt aacgtgacac
    77881 cagaaatcac ttcgcaacgg gagtgtttgt ggcttaataa tgaagccacg cgatacggtg
    77941 gcgctccggc tatatcggtt aagaagatga caccctcacc agagtccgct tcatgcagag
    78001 catcgcacat catgcggctg agcatattag agcttaaccc acgccagaaa ttcacggccc
    78061 ggcactgaag aagcgcccca tgttttttct ccagcgcatc cagtatctct tgcgcccggt
    78121 catcgtgaca ggtaattacc caacctaaca tcgccttcct ccttagccga aaatcagtct
    78181 accctgacca ttgttgataa agggtgatag ccgtcaaaac tcctctcccc caaatccaga
    78241 ggagaggaaa tagccttagc tacgcaaacc acgtccacgt tggatcagcg accaacagat
    78301 caaataaaac gccacaataa agaccaccag tacgccaaag gtagtgacca gcggaacatc
    78361 attgataccg aggaagccgt agcggaaacc actgatcata taaacgattg ggttcaggtg
    78421 cgacagccct tgccagaacg gcggcaacaa agtcagggag taaaagaccc cacccaaata
    78481 cgtgagtggc gttaacacaa aggttggcac caggctgatg tcatcgaacg ttttggcaaa
    78541 cacaccgttt agcaaacccg caagggagaa caacaccgcc gtgagcacca gggttaaggc
    78601 aacgaatacc cacgaatgca cctgaaacgg cacaaaaaac agtgaaattg ccgtcaccag
    78661 aatgccaaca aacagaccac gcgccacgcc accgccgaca tatccggcaa taatgacgtg
    78721 agtcggaacc ggcgctacca gcagctcttc aatattacgc tggaatttgg caccaaaaaa
    78781 tgatgacgcg acgttggcgt aggcgttggt gatcaccgac atcatgatca gccccggtac
    78841 gatgaactgc atgtagctga agccatgcat atcgccaata cgcgaaccaa tcaggttacc
    78901 gaaaataata aagtaaaggg tcatggtgat aactggcggc accagcgtct gcacccagat
    78961 acgcataaag cgatggatct ctttcgccca gatgcttttt aacgccaccc agtaaagatg
    79021 catcatgcgc gatctccttg tttttcatta accagtgaaa caaacagctc ttccagacgg
    79081 ttagctttgt tacgcatact taatacctga atgccctgct cacttaactg cgtaaatacg
    79141 ctgttgatcc cctgctcacg cagcacttca acttccagcg tcgcggtatc gaccagtcga
    79201 tactgatagc catcgagctt cggtaacgga cttttcggtg cgagatcgag aataaaggtt
    79261 tccgatttca gcttcgccag cagcgccttc atcgaggtat tttccaccag ctcaccgtgt
    79321 tgaataatgc cgatattgcg gcacagcatt tctgcttctt ccaggtagtg tgtggtgaga
    79381 atgatggtgg tgcctttgtc gtttaaatcc ttcaaaaagc cccacattga gcggcgaagt
    79441 tcaatatcca cgcctgcagt cggctcgtcg agaatcagta gtttaggttc atgcattaac
    79501 gcacgggcaa tcattaaacg gcgcttcatc ccgccagata acatgcgcgc acgttcgttg
    79561 cgttttcccc ataaatcgag ctgcttaaga tatttttcgc tgcggatgta cgcctctttg
    79621 cgctccacac cgtagtaccc tgcctgattc accacaattt gctgcacggt ttcaaacggg
    79681 ttgaagttaa attcctgcgg caccagtccc aactgacgtt tagcgttcac gacatctttc
    79741 tcgagatcgt aaccaaatac gctgacccgc ccggaggttt tatttaccag agagctgata
    79801 ataccgatag tggtcgattt cccggccccg ttcggcccga gaagcgcata aaaatcaccc
    79861 gcttcgacct gcaaatctat tccacgaagc gcctgaacgc cgcctggata ggttttttta
    79921 agctgttgaa gttccagtgc aatggtcata aatttttact taccttacgt tcttacactt
    79981 tatctgtggt ttaaatcgtc ccggagttgc cctatattag ccaaacgtaa ttatttggtt
    80041 acaggtcgtt aacctccatg aaagacatag atacactcat cagcaacaat gcactatggt
    80101 caaaaatgct ggtggaagag gatcccgggt tttttgagaa actggcacaa gcgcaaaaac
    80161 cgcgctttct atggattgga tgttccgaca gtcgcgttcc tgcagaacgt ttaaccggtc
    80221 ttgagccggg cgaactcttt gttcaccgta atgttgctaa cctggtcatt cacaccgacc
    80281 tgaactgcct ttccgtggtt cagtatgcag tggatgtact cgaagttgaa cacattatta
    80341 tctgtggcca ctacggttgc ggcggcgtac aagccgcagt tgaaaacccg gaactggggc
    80401 ttatcaacaa ctggctgctg catatccgcg atatctggtt caaacatagc tcattgctcg
    80461 gcgaaatgcc gcaagagcgc cgtctggata ccttgtgtga actgaacgtc atggaacagg
    80521 tgtataacct gggccactcc accattatgc aatcagcgtg gaaacgcggg cagaaagtta
    80581 ccattcacgg ctgggcctat ggcattcacg acggcttgct gcgtgatctg gatgttactg
    80641 ccaccaaccg cgaaaccctt gagcaacgtt accgtcacgg gatttccaac ctcaagctga
    80701 aacacgccaa ccacaaataa aaatgccatg ccggatgcaa cacatccggc aacttcacac
    80761 ttactcgtcc agcagaatca ctttgccgat atacggcaga tgacgataac gctgtgcgta
    80821 atcaataccg taacccacca caaactcatc cgggatcgag aaaccgataa attctaccgg
    80881 gacgttcact tcacggcggg acggtttatc cagcagcgta caaatcgcca gcgacttcgg
    80941 ttcgcgcagg cttaagatct cacgcacttt cgacagtgta ttccccgagt cgatgatatc
    81001 ttcaacaatc agcacgtcct tgccacggat atcttcatcc agatctttga ggattttcac
    81061 atcacgggtg gtggacatgc cgctaccgta gctggaggcg gtcataaagt cgacttcatg
    81121 agatacctga acttcacggc acaggtccgc cataaacata aatgagccac gcagcagacc
    81181 caccagcacc atatcgctgc cgctgtcttt gtaacgctca gtaatctgac gacccagttc
    81241 ggcgatacgc gctttaatct ccgcttcggg gatcattact tctacagtat gtttcatatc
    81301 tctaaccata tgatttaaaa acaaatcact tcgcacgggc aggttttggc accgcaacaa
    81361 agcgcaagac acgcagtata ccagtaaaac tattcattcg cggaatctgt taataaagct
    81421 cgaattgtga tgacggtcac acatgttaaa acctataatt aattgctact aaaatattaa
    81481 tgaattgaaa tggtgtctct ttatggcaat taacaataca ggctcgcgac gattactcgt
    81541 cacgctaaca gccctttttg cagcgctttg cgggctgtat ctactcattg gcggaggctg
    81601 gctggtcgcg attggcggct cctggtacta ccctatcgct ggccttgtga tgctcggcgt
    81661 cgcctggatg ctgtggcgca gtaaacgtgc cgcgctttgg ctatacgccg ccctgctgct
    81721 cggcaccatg atttggggcg tctgggaagt tggtttcgac ttctgggcgc tgactccgcg
    81781 cagcgatatt ctggtcttct tcggcatctg gctgatcctg ccgtttgtct ggcgtcgcct
    81841 ggtcattcct gccagcggcg cagttgctgc gctggtggtc gcactgctga ttagcggtgg
    81901 tatcctgacc tgggccggat ttaacgatcc gcaggaaatc aacggcacct taagcgccga
    81961 tgccacacct gccgaagcta tctcacccgt tgccgatcag gactggcctg cgtatggtcg
    82021 taatcaggaa ggtcaacgct attcgcccct gaaacagatc aacgccgata acgtccataa
    82081 cctgaaagaa gcctgggtgt tccgtactgg cgatgtgaag cagccgaacg atccgggtga
    82141 aatcaccaat gaagtgacgc cgattaaagt gggcgacacc ctttacctgt gtaccgctca
    82201 ccagcgactg tttgcgcttg atgccgccag cggcaaagag aaatggcatt acgatcctga
    82261 gctgaaaacc aacgagtctt tccagcacgt aacctgccgt ggcgtctctt atcatgaagc
    82321 caaagcagaa acggcttcgc cggaagtgat ggcggattgc ccgcgtcgta tcattcttcc
    82381 ggtcaatgat ggtcgactga ttgcgattaa cgctgaaaac ggcaaactgt gcgaaacctt
    82441 cgccaataaa ggcgtgctca atctgcaaag caatatgcca gacaccaaac cgggtctgta
    82501 tgaaccgact tcgccaccga ttatcaccga taaaaccatt gtgatggcag gttcagttac
    82561 cgataacttc tcaacccgcg aaacgtctgg cgtgatccgt ggttttgatg tcaacaccgg
    82621 ggagctgctg tgggcttttg atccgggcgc gaaagatccg aacgcaatcc cgtctgacga
    82681 acacaccttt acctttaact cgccaaactc gtgggcacca gcggcctatg acgcgaagct
    82741 ggatctggtg tatctgccga tgggcgtgac cacgccggat atctggggcg gtaaccgcac
    82801 accggaacag gaacgttatg ccagctcgat tctggcgctg aatgccacaa ccgggaaatt
    82861 ggcgtggagc taccagaccg ttcaccacga cctgtgggat atggatcttc cggcacagcc
    82921 gacgctggcg gacatcaccg ttaatggtca gaaagtgcca gttatttacg ctccggcgaa
    82981 aaccggcaac atttttgtgc tcgatcgtcg taatggcgaa ctggtggttc cggcaccgga
    83041 aaaaccggtt ccccaaggtg ctgccaaagg cgattacgtt acccctactc aaccattctc
    83101 ggaactgagc ttccgtccga cgaaagattt gagcggtgcg gatatgtggg gagccaccat
    83161 gtttgaccaa ctggtgtgcc gcgtgatgtt ccaccagatg cgctatgaag gcattttcac
    83221 gccgccatct gaacagggca cgctggtctt cccgggtaac ctgggaatgt ttgaatgggg
    83281 cgggatttcc gttgatccaa atcgtgaagt ggcaattgcc aacccaatgg cactgccgtt
    83341 tgtttcgaaa ctgatcccac gcggtccggg taacccgatg gagcagccga aagatgccaa
    83401 aggcacgggt acggaatccg gcattcagcc acagtacggt gtaccgtatg gtgtcacgct
    83461 caacccgttc ctctcaccat ttggtctgcc atgtaaacag ccagcatggg gttatatctc
    83521 ggcgctggat ctgaaaacca atgaagtggt gtggaagaaa cgtattggta cgccgcagga
    83581 cagtatgccg ttcccgatgc ctgtaccggt gccgttcaat atgggtatgc cgatgctggg
    83641 cgggccaatc tccacggcgg gtaacgtgct gtttatcgcc gctacggcag ataactacct
    83701 gcgcgcttac aacatgagca acggtgaaaa actgtggcag ggtcgtttac cagcgggtgg
    83761 tcaggcgacg ccaatgacct atgaagtgaa tggtaagcag tatgtggtga tctccgcagg
    83821 cggtcacggt tcatttggta cgaagatggg cgactatatt gtggcttatg ccctgccgga
    83881 tgatgtgaag taagacttgc tcagattgct gacaacgtgc gcgttgttca tgccggatgc
    83941 ggcgtgaacg ccttatctag cctacaaaat tgtgcaaaat caatggattg cacaggtagc
    84001 gtaggcctga taagcgtagc gcatcaggca gatttgcgtt tgtcatcagt ttaatgcccg
    84061 gagagatccg ggcatattcc cgaatacggt ctttttatac cgtaaaccct aacatcatcc
    84121 ccgtatcttc atgctccagc agatggcagt gcgccatata agcatgttct ttcggtgcgt
    84181 cgtgattaaa cttcaccagc acttcactga cattgccttc taccttaacg gtatctttcc
    84241 agcccgcgcg atgagccgct ggcggtttgc cattttctga caagatacgg aactgcgtgc
    84301 cgtgaatatg gaacggatgc agcatcatgt cgcccacgcc agagataacc caacgttcgt
    84361 actgcccttt cgccgccgca aacatcggct tattcatatc aaacgcctga ccgttgattt
    84421 tattggcatg gtggaaatcg aacttcccgc cgtggttcat atgattcata ttgccgtgcc
    84481 ccatatggcc catcatctgg ctgtgatcca ttccggccat cgcctgatcg ccatatttct
    84541 ccattagcat ctgcatcccc atcatatcga gcatcgggtc catagaaagt tgcagcttgc
    84601 gtaccgtcag cccttccagc gaaggtaacg caggcaggct acttaatgtg tctggcaaag
    84661 caccggaggc actaatagca atcggctgaa tccgcattac cggatgcggc ttgtcaaacg
    84721 gtgcaatcgc catccccatc tggctgaccg gcagcgtcac caggtcaaag ggtttgttgt
    84781 cgttaacctc caccagcact tcaaaacgct cgcccatcag caccggcagc tcgttcacct
    84841 tcaccggttc aggtagcaga ccaccgtcgc tggcaatcac ataaagcggg cgattgtcgc
    84901 tggtggcgaa attgagcgag cgggcattac agccattgag caaacgcagg cgcagccaac
    84961 cacgcggggc agcgtgttgc gggtagattg caccgttggt cagcaacgta tcgccaaacc
    85021 agcccacggc ggcggtcatc acatccagtt gataatcaat ctgcccgtcg gcgctaaatt
    85081 tcttatcctg aacgatcacc ggaacatcat cgatacccca ctgttttggc agcattaatt
    85141 tcaggatctc gtcatcttca atcaccacca gaccagccag ccccatcgcc acctgtcgcc
    85201 cggtcttgcc atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca
    85261 acgtcaccga gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacttcac
    85321 ccggtacttc cagcccgtgc cagtgcaacg tcgtctcttc cgtcagttgg ttatagatat
    85381 caaccgttac cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat
    85441 agccccaggt agttgcagtt ttctcgccaa aggtagactg acctgcgcca atagttaact
    85501 gaatgcgatt acgagcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg
    85561 ccgcaaagac tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat
    85621 atttcaagaa atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa
    85681 tcaaacgttg agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat
    85741 cgacaataat ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct
    85801 ggtagaagca atgaagacgt ttttcagaac agttttattc ggcagcctga tggccgtttg
    85861 cgcaaacagt tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt
    85921 ttttgtcttt ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg
    85981 cgcactggtc tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga
    86041 catgaaagcc ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc
    86101 taaaaagtgc aaagccctgg ctcgcgattc cttaagcctg cttgcctacg tcaaataatc
    86161 cctgatacct ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt
    86221 tgcgcccttt ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca
    86281 gtggcatgaa acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta
    86341 tcatgaaaag accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt
    86401 aatggcgctg gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat
    86461 gatgacccat gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg
    86521 cggcgacggt gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat
    86581 ggtggaaatc gatgcgggtg tcgtatcgtt ctgccgtcag tatctaccca accataacgc
    86641 cggtagctac gacgatccgc gctttaagct ggtgatcgat gatggcgtca atttcgttaa
    86701 tcaaaccagc cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg
    86761 cgaaagcctt ttcacttcgg cattttatga aggctgcaaa cgttgcctga atcctggcgg
    86821 tatcttcgtc gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca
    86881 tcgcaaactc agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta
    86941 ttacggcggt atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc
    87001 aaccgaaatt attcaggcgc gttttctcgc ctctggcctg aaatgccgtt attacaatcc
    87061 ggcaatccat acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca
    87121 gccgtcctaa ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct
    87181 gaccaaaagt ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg
    87241 cgacggttat attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct
    87301 gtcagaaacc tgttccatta tcggggctaa tattcttaac atcgcccgcc aggattacga
    87361 accacagggt gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat
    87421 cgacaaaaca gaacaccccg gcccactgcc agaaacggtc gttgcccatc ttgataaaag
    87481 tcatatttgc gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg
    87541 cgccgatatt gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct
    87601 gatccaccag cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttacccg
    87661 cgacattaac ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat
    87721 gtctgacgat atgaaggcgc tgtatgacat ggtggatgtg aacgtctatc aggaaaatat
    87781 cttccatacc aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa
    87841 accggaagac ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat
    87901 gcgcgagatt tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca
    87961 ggctccgcca gatttattta cttctgctgc acgaaattac ggtaagccgc cacgacctgc
    88021 agaaaatcct caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct
    88081 tccatttcat cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcaccgtcc
    88141 atccacaggg tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg
    88201 tgcgctgctt gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac
    88261 cagtgcccga ccacttcatg ccccatggac atacgcacct ttaccactcc ggtggtatcg
    88321 cgcagaaatt cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat
    88381 tgcgccattg atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt
    88441 tagaaataaa aaatgcccgg gaatctcatt caccgggcat tgtgtcgttt atgcgcagcg
    88501 cgtgcgctga ctttttaaac tgcagtctgg aaaatcacac catcggcttt ttcagtgtac
    88561 tgagaaagct ggttgaagtt caggtaacgg taagtatcaa cggcagtttt atccacctgc
    88621 gccacgtagg tctggtactc ttccggcgtc ggtagtttgc caatcagcgc cgcaacagca
    88681 gccagttccg cagaagccag gaagacattc gcgccagtcc ccagacggtt cgggaagtta
    88741 cgggtagagg tggaaaccac cgttgcaccg tccgccacac gcgcctggtt acccatacac
    88801 agggaacagc cagggatctc gatacgcgca ccactcttac cgaagacgct gtagtagcct
    88861 tcttcggtca actgtgcggc gtccatacgg gttggcggtg ccacccacag gcgggtcggc
    88921 aactggcctt tatgcgcatc cagcagttta cccgcagcac ggaagtgacc gatgttggtc
    88981 atgcaggaac cgataaacac ttcgtcgatc ttctcaccct gtaccgcaga cagcggacgc
    89041 gcgtcatccg ggtcgttcgg agcacacagg attggctctt taatatccgc cagatcgatg
    89101 tcgatcactg ccgcgtattc cgcatctgca tcggcttcca gcagctcagg attcgccagc
    89161 catttttcca tgccctgaat acgacgttcc agggtacgac gatcgccgta accttccgcg
    89221 atcatccact tcagcaggac gatattagag ttcaggtatt cgatgatcgg ttctttgttc
    89281 agcttgatgg tacaaccggc agcagaacgc tcggcggacg catcggtcag ttcaaatgcc
    89341 tgctcaactt tcagatccgg cagaccttca atttccagaa tgcgacctga gaagatgttt
    89401 ttcttgcctt tcttctcaac ggtcagcaga ccttgtttga tcgcatacag cggaatagcg
    89461 tgtaccagat cgcgcagggt gatgcccggc tgcattttgc ctttgaagcg caccagaacg
    89521 gattccggca tatccagcgg cattacgcct gttgcggctg caaatgccac cagaccagaa
    89581 cccgccggaa aagagatacc gatcgggaaa cgggtatggg agtcaccacc ggtaccgacg
    89641 gtatccggca gcagcatacg gttcagccag gagtggatta cgccgtcacc cggacgcagc
    89701 gacacaccgc cacggttcat aatgaagtcc ggcagcgtgt ggtgcgtgtt cacgtcaacc
    89761 ggcttcggat acgccgctgt gtggcagaaa gactgcatca ccaggtcagc cgagaagccc
    89821 aggcacgcca ggtctttcag ttcatcacgg gtcatcgggc cggtggtgtc ctgggaacct
    89881 acagaagtca ttttcggttc acagtacgcg cccggacgaa tgcctttcac gccacaggca
    89941 cggcctacca ttttctgcgc cagcgagaag ccgcgatcgc tctcagcgac atctttcgcc
    90001 tgacggaaca catcactgtg cggcagacca agtgcttcac gcgctttggt ggtcaggcca
    90061 cgcccgataa tcagcggaat acggccgcca gcacgcactt catcaatcag cacgtcggtt
    90121 ttcagttcga aggtcgccag cagttcgcct gtttcgtggt tacacacttc acctttgtac
    90181 gggtaaacgt caatcacgtc gcccatgttc aggttagaga cgtcgacttc gattggcagt
    90241 gcacccgcgt cttccatcgt gttaaagaag attggtgcaa ttttaccgcc gaggcacaaa
    90301 ccaccgccgc gtttgttcgg cacatgtgga atatcatcgc ccataaacca cagaacggag
    90361 ttggtggcgg atttacgcga tgaacccgta cccacaacgt caccgacgta cgccagcggg
    90421 aaacctttct gttgcagagc ttcaatctgt ttgatcgggc caacaacacc tggctggtct
    90481 ggctcaatac cttcacgggc gtttttcagc atcgccagcg cgtgcagtgg gatatccggg
    90541 cgcgaccacg catccggtgc cggagaaagg tcatcggtgt tggtttcgcc agtgactttg
    90601 aagacggtaa cggtcagttt ttcagccagc gccgggcgat tcaggaacca ttcggcatcc
    90661 gcccaggact gcattacctg cttcgcatat tcgttgcctg ctttggcttt ctcttctacg
    90721 tcatagaagt tatcgaacat cagcagcgtg tgagaaagtg ctttggcagc gatcggtgcc
    90781 agtttggcat catccagcgc gtcgatcagc ggatgaatgt tgtaaccacc ctgcatggtg
    90841 cccagcagtt cgatggcttt ttccggagtc agcagtgggg atttggcttc acctttcgcg
    90901 acagcagcca ggaagcctgc tttgacatag gcggcttcat cgacgccagg gggaacacgg
    90961 ttggttaata gatctaacag gaattcttct tcgcccgcgg gcgggttttt cagcagctct
    91021 acaagtgcgg ccatttggtt tgcatccagg ggtttgggcg caatcccctc agcggcacgc
    91081 tcagctacgt gcttacggta ttcttctagc acgacggttc tcctcgctct cattgtcata
    91141 gtgcggcagg cgattctctt cacgctcctg tgagacagca gtttgtaggg taaatgccca
    91201 gtaccgcaac gggcagaata gcaggatttt gacgatgtgt taatctgttt acaaaaaagc
    91261 aacataaaaa agtggctgaa tcgttaagga tggtctagag attgtttccc tccgtaaaaa
    91321 cttcgttaat tcgcatagtg ataatcacct ttctcaacga atcccaagat tagtcaaaat
    91381 ttaaactacc gcctctttat actcagattt acagcacctg cgggtggcag ttcgccgacc
    91441 attgcgattt ccttgagatc cgaattatga aaatgacttt gccgtttaaa ccccatgtgc
    91501 tggcgctaat ttgcagtgcc gggctttgtg ccgcctctgc cgggctatat ataaaaagcc
    91561 gcacagtgga agcgcctgtg gaaccgcaat cgacacaaca gactgcgcct gacatcaccg
    91621 cagttacgct tcctgcaacg gtttccgcac cgcccgtaac gcccgccgtc gttaaatccg
    91681 cattcagcac tgcacaaatc gatcaatggg ttgcacctgt cgcgctatac cccgactctc
    91741 tgctttcaca agtgttaatg gcatcaacct atccggcaaa cgttgctcaa gcagtgcaat
    91801 ggtcgcacga taatccactt aaacaaggcg atgctgctat tctggcggta tctgaccagc
    91861 cgtgggacgc cagcgttaaa tcactggtgg cctttccaca attgatggcg ttgatgggcg
    91921 aaaacccgca atgggtgcaa aacctgggcg atgcttttct ggcgcagccg caggacgtga
    91981 tggactcggt acagcgattg cggcaactgg cgcaacaaac cggctcgctg aagtcatcaa
    92041 ccgaacagaa agttattacc acaacgaaga aaactgtacc ggtaacacag acagtcacgg
    92101 ctcccgtcat accatccaat accgtttcaa ctgccaaccc cgtcattaca gagcctgcaa
    92161 caaccgtcat ttccattgag cccgccaatc ctgatgtagt ctatattccc aactacaacc
    92221 caaccgtggt ttacgggaac tgggccaata ctgcgtatcc gccggtttat ctgccaccac
    92281 cagccggaga accgtttgtt gacagctttg tgcgcggatt cggctatagc atgggcgttg
    92341 ctaccactta cgcactattc agcagcatcg actgggatga cgacgatcat gaccatcatc
    92401 atgacgatga ttatcatcac cacgatggcg gtcatcgtga cggtaatggc tggcaacaca
    92461 acggcgacaa catcaatatc gacgtcaaca atttcaaccg tatcaccggt gagcatctta
    92521 ctgataagaa tatggcatgg cggcacaatc caaactaccg taatggtgtg ccctatcatg
    92581 atcaggatat ggcaaagcgg tttcatcaaa ctgatgtcaa cggcggaatg agtgccacgc
    92641 agctacctgc tccaacacgc gacagccagc gtcaggcggc agcaagtcag tttcagcaac
    92701 gaacacacgc cgcccccgtc attacacgag atacccaacg tcaggcagcg gcacagcggt
    92761 ttaatgaagc tgaacactat gggagctatg acgacttccg cgacttcagc cgtcgccaac
    92821 cactgaccca gcaacaaaag gacgccgctc gtcagcgtta tcagtcagct tctcctgagc
    92881 agcgccaggc agttcacgag aaaatgcaga ctaacccgca gaaccagcag cgaagagagg
    92941 cagcgcgtga gcgcattcag cccgcctcgc ctgagcagcg ccaggcagtc cgcgagaaaa
    93001 tgcagactaa cccacagatc cagcagcgaa gagaggcagc gcgtgagcgt attcagtcag
    93061 cctcgcctga gcagcgccag gtgtttaagg aaaaagtaca gcagagccca ctggaccaac
    93121 agcaacgtga taacgcccgc cagcgtgttc aatcggcatc acctgaacaa cgtcaggttt
    93181 ttcgggagaa agttaaggag agccacccac aacgtctaaa cgacagtaac catactgcca
    93241 ggctaaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc ggagcaaggc
    93301 gactggaaag gtaaattaca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg
    93361 ccggatgttc cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcggt
    93421 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
    93481 atggatggtc agcgcgatgt cttcagcgtc acaacccatt tcgattgcca ggccgatttc
    93541 acccagcagc tcgccgccgt tggtaccgac aatcgcacca ccgatcacac ggtgagattc
    93601 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga
    93661 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
    93721 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
    93781 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
    93841 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
    93901 ggtacgcagc tgtttgtcaa cgcggatgaa accacgatcg tcaacttcca cgccagcttt
    93961 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
    94021 acgctgcggt tcagctggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt
    94081 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    94141 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    94201 cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc atttccagac cgatgatacc
    94261 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc
    94321 ccagatacgc ggatcttcat gcggaataaa cggcagttga atcgggcgag aacccgctgc
    94381 aatgatcgcg ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa cttccagggt
    94441 gttagccccg gtaaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    94501 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    94561 gatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    94621 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    94681 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    94741 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    94801 agtactcatc atgacctcta tatatttatc tccggcgggt ctgacgtttt ttttatgacg
    94861 cccattatcc accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta
    94921 aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta
    94981 ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc
    95041 agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg
    95101 gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca
    95161 caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat
    95221 cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg
    95281 catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg
    95341 agacagctcg atgatgcctt tcttgttgac gtctttgaat accggaacaa ccaaaccgtt
    95401 cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc
    95461 ttccgacagc gaactgttga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat
    95521 gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg
    95581 ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtgag taacatgcgg
    95641 gatcatcacc cagttacggc tcaggttcgc accagaaatt ttctggatac ggcccagttc
    95701 cacttcttcg atttcaccaa acttgctgaa gtccaccttc ggccacggca gcatgcctgg
    95761 gataccgccg ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta
    95821 agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag
    95881 gttaacgcca aactcgcgtg ccagacggcg gatcagcgga gtcgcgtgaa cataagcgtc
    95941 gttttcagca aattcagatt tgccttccgc tttcgcagct ggtgctgctg ccggggcttc
    96001 agcttttgct gccggagccg gcgctgccgc ttcctgtttc gcaggagctg ccgcaggcgc
    96061 tgcgccttca acttcgaaga tcataatcag cgagccagtt ttcactttat cgccaacgtt
    96121 gactttcagt tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttgtc
    96181 gccttctacg gtaatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac
    96241 ttcggtcact tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc
    96301 tgctggtgca ggggccgctg ccggagccgc ttcctgttta gccgccggag ctgccgcgcc
    96361 tgcttcaccc gcgacttcga agaccataat cagcgagccg gtagacactt tgtcgccggt
    96421 gttcactttg atttctttca cggtgcctgc aaacggagcc ggaacttcca tagaagcttt
    96481 atcgccttct acggtgatca gcgactgctc agcttcaact ttgtcgccaa ctttcaccag
    96541 gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc
    96601 ggcagccgcg ggtgctgctg ccggagctgc ttctttcttc tcttctgcct gagcaggtgc
    96661 agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgggtttt
    96721 atcgccaaca gagactttga tctctttaac gatacccgcc tgcggagacg gaacttccat
    96781 agaggctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac
    96841 tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc
    96901 gatagccatt attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg
    96961 ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt
    97021 tcgcccagcg ccgcaaccac gacataagaa gcatcaactt cgaagtggtg acgcaggttc
    97081 tcacggctgt cggaacgacc gaagccatca gtacccagta cgcggtagtc gtcagccggt
    97141 acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc cactgccgga
    97201 gcgtcgttca tcacctgagc gatatacgga acgcgcggag tttccagcgg gtgcagcatg
    97261 ttccagcgtt cacaatcctg accatcacgc gccagctcgg tgaaggaggt cacgctataa
    97321 acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc
    97381 aggatagaac cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt
    97441 ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg catgtgatag
    97501 ttttcgttca gcgtagtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc
    97561 tccagaccgt catgcatgat gacagcaact tcgtaagcgt aagccgggtc gtaagagata
    97621 cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttcg
    97681 ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtcg
    97741 ccagccgccc agcacagatc gccaatacgc tggaagccga acatcgagta atagatgtag
    97801 aacgggatca tcggcagatt gttggtgctg taagaggtcg ccgctgccag ccaggaacac
    97861 cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag
    97921 taagcaacct gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca
    97981 atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc
    98041 agacgatctt tgatcgactt gttcttcagc attacgttca gggcacgaac gaaagcgata
    98101 gtggtagaga tctctttgct ttgctcttcc agcagcgcgc cgaagtcttg caggctcggc
    98161 agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga
    98221 cgctgagcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt
    98281 ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacgccg
    98341 tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg
    98401 ccgtaacctt taatggtatg agcaaggatt accgtcgctt tgcctttggt ttcctgcgct
    98461 ttcttaaatg cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc
    98521 tgttcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt
    98581 tcacgaacgt acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg
    98641 ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta
    98701 ccccacatca ctttgatcac gttccagcca gcaccttcga agatgccttc cagttcgttg
    98761 atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg
    98821 aagaccaggt tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt
    98881 tcgtccattt caccgtcgcc gaggaacgcg taaacggttt gtttagaggt atctttcagg
    98941 ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccaga
    99001 cccatagata cggtcgggaa ctgccagaat tccggcatca atttcgggtg cggataggaa
    99061 gagaggccat tgccgtgaac ttcctgacgg aagttatcca gctgctcttg agtcagacga
    99121 ccttccagga aagcacgagc gtacacgccc ggggagatgt ggccctggaa gtaaaccagg
    99181 tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg
    99241 gttgcggaag actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca
    99301 cgcagaaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc
    99361 agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata
    99421 ccggtgcctg cggctacgtt gacaccgcct ttgcgggctt cagcaagcag ttggtcgatc
    99481 agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag
    99541 tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta
    99601 tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc cggaaagctc
    99661 aataagacag gttctacgtt tagttgccgc gcttttatat gcgcttgatt tacaacatct
    99721 tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga
    99781 acgctcacgg cggctttctt cacggcttct gtcgagcaaa atttcttcga taaaggccag
    99841 atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat
    99901 gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa
    99961 gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac
   100021 cacattgtgg gccgcttcgg tgacggcaat ctgatactgg agtacggcgt ttgattccgc
   100081 gtccagatcg ccagactgct gcgccagttc tatggcgtgg tggagttcac ggatgcgttc
   100141 cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg
   100201 tcgtgtttcg agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg
   100261 atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag
   100321 caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aatgagggac gggagacgtc
   100381 aaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc
   100441 gaggatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt
   100501 gctgtaggcc atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt
   100561 tcatgtctgt gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg
   100621 aaatcatgaa actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac
   100681 tatgaacaaa tgtagacttt acgtaggaaa gggatttttt aacgattatt aaactataaa
   100741 aatccgaatt gaaccgattc acttaccaat tttgtgattt ttaattcaat taaaacgaat
   100801 ttaaattcat tctgcatatt gagaggggtt gaggctgagc tttacaaacg gtttcttttt
   100861 aagcaactca tcttcaacca tgcataaagc gggtgcattc gctgccgcat accattattc
   100921 ttgatctgac ggaagtcttt ttgtaacaat tcaaacttct ttgatgtaaa caaattaata
   100981 caacaaacgg aattgcaaac ttacacacgc atcactgcgt agatcaaaaa aacaatcacc
   101041 gcacgaggtt tcatgatgga aggtcaacag cacggcgagc agctaaagcg cggccttaaa
   101101 aaccgccata ttcagcttat cgcgctgggt ggcgcgatag ggaccgggtt attcctgggt
   101161 agcgcctccg taatacagtc cgcagggcca gggattatcc tgggatacgc cattgctggt
   101221 tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca
   101281 ggctccttta gccactttgc ttataaatac tggggcagtt ttgccggttt cgcctctggc
   101341 tggaactact gggtactgta cgttttagtt gccatggcag agctgactgc tgtgggtaaa
   101401 tacattcagt tctggtatcc ggaaatccca acctgggttt ctgccgccgt gttctttgtg
   101461 gtgattaacg ccatcaacct gaccaacgta aaagtgtttg gtgagatgga gttctggttt
   101521 gccattatca aagttattgc ggtagtagcg atgatcatct tcggcggctg gctgctgttc
   101581 agtggtaacg gcggtccgca ggcaagcgtt agcaacctgt gggatcaggg cggtttcctg
   101641 ccgcacggct tcaccgggct ggtgatgatg atggcgatta tcatgttctc gttcggtggt
   101701 ctggaactgg tggggatcac cgcagcagaa gctgataacc cggagcaaag tatcccgaaa
   101761 gcgactaacc aggttatcta ccgcatcctg attttctata ttggttcgtt agccgttctg
   101821 ctctcactga tgccgtggac ccgcgttacc gccgatacca gtccgtttgt gctgatcttc
   101881 cacgagttag gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg
   101941 ctctccgtgt acaacagctg cgtatattgc aacagccgta tgctgtttgg tctggcacaa
   102001 cagggtaatg cgccaaaagc gctggcgtct gtcgataaac gcggcgtacc ggttaacacc
   102061 attctggtgt ctgcactggt aacggcattg tgcgtactga ttaactatct tgctccggaa
   102121 tccgcatttg gcctgttaat ggcgctggtg gtatctgcac tggtgatcaa ctgggcgatg
   102181 attagcctgg cgcatatgaa gttccgtcgc gccaagctgg aacaaggcgt ggtaactcgc
   102241 ttccctgctc tgctttatcc gctgggtaac tggatctgcc tgctgtttat ggcggcggta
   102301 ctggtgatta tgctgatgac cccaggaatg gcgatttcgg tatacctgat cccggtatgg
   102361 ctggtggtgt taggtatcgg ctatctgttt aaagagaaaa ccgcaaaagc cgtaaaagca
   102421 cattaatctc tctacgccct cacccgtaca gggtgagggc aataatcttt acaccaacgc
   102481 cccgtaaatc gtcagtagcg caatcaccac cacgaccacg aacgaggttt tcttcgccat
   102541 tgaaaccgct gccttcggcg tctccacctt atcgacatgc ggttcacgcg ccagagagaa
   102601 ctgcgccaga cgcgttaata cctgatattg cgaagtacgg aaatcaccca gcgaagcaaa
   102661 ccaggccggt aacgctttct caccatgacc gatcaaggca tataccacac ccgcaagacg
   102721 aaccggcacc caatccagta catgaagcac tgcatcaatg ccggactgta aacgatgatg
   102781 cggcgtctgg tatcgcgcca gccagtattg ccatgcacgc aagaacgcat accccatcag
   102841 cgtaacgggt ccccaggttc cccccacaat cagccagaac agcggtgcaa gataaaaacg
   102901 aaagttaatc cacagcaatg cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc
   102961 tgccgggacg ccgtgaatca tggtgagttc gccagccatc gtggcacggg catggctatc
   103021 attacgtgaa gcagctgtca gataagcatg ataatgaaga cgaactttac ctgcgccaat
   103081 acacagcaaa ccaatcagca gccacaccag cagcgtggga acgttgaaca atactccctg
   103141 caatgcgcgt aacagtaaaa aagtcacgcc catcgcaata atggtcatgc ctaacgtgcg
   103201 cccgagagaa aaatgtttca cccgccgaaa gaacgcttca agacgatgat caagctgcca
   103261 gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc agtaaggttg taaatagcgt
   103321 catgttgtct ccttgctgac cagcgcacga aaccgtgccc aatcaaatgc aggaccggga
   103381 tcggttttcc gatccggcgc aatatcacaa tggcccgtca tgttattagc gatatccgga
   103441 tagcaatcaa tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc attggtatac
   103501 gccagcgtat cggtgccttc aagctcaatc ccaatagaaa aatcattgca gcgttcgcgc
   103561 ccctgatact gagagactcc cgcatgccat gcacgtttat cgaaaggaac atactggact
   103621 atttcaccat cacggcgaat caaacagtga gcggagacgc gcaaatgggc gatctcagca
   103681 aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat ccacggaccg
   103741 ccaaactcgc ctggcggcag gctaatattg tgcaccacca gcagggtggg tgtttcgtca
   103801 tccgggcggc aatcgtaatg tggtgaggga acgcggcgcg cgccaaccaa ccacccctgt
   103861 tctaacaaca tgcaggatct ccttatatgt ggtgctaata cccggttcag agtagcatgt
   103921 ttctacctta tgattcgtta gctatctgga gttttaacat gccgcctcgc cgctacaacc
   103981 ctgacacccg acgtgacgag ctgctggaac gcattaatct cgatatcccc ggcgcggtgg
   104041 cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc caacaatgac attacggcaa
   104101 aacttttacc ggaaaattct cgctctcatg ccacggtgat cacccgcgag aatggcgtat
   104161 tttgcggcaa acgctgggtt gaagaggtgt ttattcaact ggcaggcgac gatgtcacca
   104221 taatctggca tgtggatgac ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg
   104281 aaggcccatc ccgcgtgctg ttaacgggtg aacgcactgc gcttaatttt gtgcaaaccc
   104341 tttcaggagt tgccagtaag gtacgccact atgtcgaatt actggaaggc accaacacgc
   104401 agttgttgga tacgcgcaaa accttacccg gcctgcgttc agcgctgaaa tacgcggtac
   104461 tttgcggcgg cggagcgaat caccgtctgg ggctttctga tgccttcctg atcaaagaaa
   104521 accatattat tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcatc
   104581 cggatgcgcc agtagaagtc gaagtagaga atctggaaga acttgatgaa gccctgaaag
   104641 caggagccga tatcatcatg ctggataact tcgaaacaga acagatgcgc gaagccgtca
   104701 aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa cgtcactgac aaaacactgc
   104761 gcgaatttgc cgaaacgggc gtggacttta tctccgtcgg tgcgctaact aaacacgtac
   104821 aagcactcga cctttcaatg cgttttcgct agttgctgac agatttatcc gctccatagt
   104881 gggcggataa tcaccccttc cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt
   104941 cgctgcaacg gaataaaaaa cctcggaagc agctttcccg tttcgttttt tgcccctcgc
   105001 ctgtgctgat ggcgtttgcc aaagtagcgc caacccaatc aaggagcgaa acagatggac
   105061 aagcaacgcg gttttacact tatcgaactg atggtggtta ttggcatcat tgccatttta
   105121 agcgccattg gtattcccgc ttatcaaaac tacctgcgca aagccgcact caccgacatg
   105181 ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga
   105241 ttagatacct gcgacggtgg cagcaatggc attccctcgc ctaccaccac ccgctatgtt
   105301 tcagccatga gtgtggcaaa aggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg
   105361 ctaagcgtcg tcatgacgcc gggttgggat aacgcaaacg gcgtcaccgg ctggacgcgc
   105421 aactgcaata ttcaaaatga cagcgcattg cagcaagcct gcgaagatgt cttccgcttt
   105481 gatgacgcca actaaggagc ggcaatgaat attccacagc tcacggccct gtgcctgcgt
   105541 tatcagggag tcttgctgga tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc
   105601 ccctcacatg aattgctgga cgcattgcat ttcgctacca ccaaacgtat tgagatcacc
   105661 tgctggacgc gccaacaaat ggaaggtcac gccagtcgca cacaacagac attgcccgta
   105721 gctgttcagg agaagcatca gcccaaagca gagttgctga ctcgaacgtt acaatctgcg
   105781 ctggaacaac gcgcgtctga tattcatatc gaaccagcgg acaatgccta ccgcatccgc
   105841 ttgcgtatcg acggcgtatt gcatccttta ccggatgttt caccggatgc cggagtcgca
   105901 ttaacagcca gattaaaagt gctgggaaac ctggatattg cggaacatcg cctgccgcag
   105961 gacgggcaat tcactgtcga actggcagga aacgccgtct catttcgtat tgcgacctta
   106021 ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac agcaggtgag ccaggcactg
   106081 gatgttaaca cgctgggaat gcagccgtta caactggcgg actttgctca tgccttgcaa
   106141 caaccacagg gactggtgct ggtaactggc cctaccggca gcggcaaaac ggtcacgctt
   106201 tatagtgccc tgcaaaagct gaataccgct gacattaata tttgtagcgt cgaagatccg
   106261 gttgagatcc ccatagccgg actaaaccag acgcaaatcc atccgcgtgc cgggctcacc
   106321 tttcagggcg ttttgcgtgc gttattgcgc caggatcctg acgtcatcat gatcggagag
   106381 atccgcgatg gcgaaacggc agaaattgcc attaaagccg cgcaaaccgg tcacctggtg
   106441 ttgtccacgc tacacaccaa ttccaccact gaaacgctgg tacgtttaca gcaaatgggg
   106501 gtcgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgt
   106561 aaactttgcc cacattgtcg ccgacagcaa ggggagctca tccatattcc agacaatgta
   106621 tggccgtcgc cgctgcccca ttggcaggca cccggttgtg tacattgcta ccacggtttt
   106681 tatggtcgca ctgccttatt tgaagttctg cccataacac cggtcatacg tcagcttatt
   106741 tccgctaata ccgacgttga atcgctggaa acgcacgccc gacaggcggg tatgcgaacg
   106801 ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc
   106861 cgcgtattgg ggatgccgca tggcgagtaa gcaactctgg cgctggcatg gcataaccgg
   106921 cgacggcaat gcgcaagatg ggatgcgatg ggcagagagc cgtgctttgc tgctcatggc
   106981 actacagcaa cagatggtta ctccactaag cctgaagcga attgctatca attctgcgca
   107041 gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac ttaaagccgg
   107101 gttaactctt tctgaagggc tggctctgct ggcggaacag catcccagta agcaatggca
   107161 agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt
   107221 attaccctgg tcagaggtat ttccgccgct ctatcaggcg atgatccgca cgggtgaact
   107281 gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaag cccagcgtca
   107341 gttgaccgac aaagtaaaat cagcgttacg ttatcccatc atcattttag cgatggcaat
   107401 catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac
   107461 cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg
   107521 cgaatggggc tggctgctgg tgttgttcgg cattatgctg acgatagcca ataagttgct
   107581 gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca tcccgattat
   107641 ggggtcactg atgcggggac aaaaactcac gcagatcttt acgattctgg cgctgactca
   107701 aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa tgcgctgccc
   107761 gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg
   107821 gctggcgtta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag tgagaacggg
   107881 agaggcctcc ggctcgctgg atctcatgtt agacaacctc gcccatcatc atcgggataa
   107941 cacaatggcg ctggcggata acctagcagc cttactggaa ccggcgttgc tgatcataac
   108001 gggaggaatt atcggtacgc tggtggtggc gatgtatctg ccaattttcc atttaggcga
   108061 tgcgatgagt gggatgggat aacgctggcg tgttgctcca caccagcgtt ggaagattac
   108121 aggttgttga agatgcggtt ttcttgttcc tgcacacgga taaatgtggt gcgcttggtc
   108181 agttctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata
   108241 tcacgcgcgg tattttcaac cgggcctcgc agcggcagct taacggtttt accttctgct
   108301 gcgcgatatt cagcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg
   108361 tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg
   108421 cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc tttcgcgaca
   108481 tcgcccggcg tggtgcagcc accatcgctg acgatcattc cgcccagacc gtgcgcagca
   108541 tcggcacatt cgattaccgc agaaagttgc ggataaccga cgcctgtttt gacgcgagtt
   108601 gtacaaacag aacctgggcc aatgccaact ttaacgatat cggcaccgga gaggataagc
   108661 tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct
   108721 tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata
   108781 caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca
   108841 tcagacgtac cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata
   108901 aacgcttgcc actcttcgac agaatagtgt ttatgcacag cagtcaaaat atcaaaagaa
   108961 gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc gataatcggc
   109021 acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg
   109081 gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc
   109141 agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg
   109201 caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct
   109261 ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat
   109321 ttcgctttcg tgcgctcatt tttaacgttt tgataatcat gatgaattcc cgggaatata
   109381 aattatgagg tatatagttg ccttaacggg aggcattggc agtggcaaga gtaccgttgc
   109441 caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt
   109501 ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg ctaacatgat
   109561 tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga
   109621 agagaaaaac tggcttaacg acctgctgca tccgctgatt cagcaagaga cgcaacacca
   109681 gatccagcaa gcaacctccc cctatgtact gtgggttgtg ccattgctgg tagaaaactc
   109741 actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa
   109801 gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtc gaacaaatcc ttgctgctca
   109861 ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc
   109921 ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca
   109981 gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat
   110041 gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta
   110101 cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgttagtga attactggat
   110161 gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttgaccg gcagcaacgt
   110221 aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt
   110281 cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg
   110341 cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc
   110401 agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgactgc
   110461 caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg
   110521 gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag
   110581 gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat
   110641 ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa
   110701 aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg
   110761 tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc
   110821 agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct
   110881 gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa
   110941 gaaccaaagc agtgacattt gtctgatgcc gcacgtaggc ctgataagac gcggacagcg
   111001 tcgcatcagg catcgtgtgc aaatgtcgga tgcggcgtga acgccttatc cgacctacaa
   111061 aaaaaaattt atcaatgagt tagaaatccc tttaggcctg ataagacgcg ccagcgtcgc
   111121 atcaggcatc gtgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacaaaaaa
   111181 aatttatcaa tgagttagaa atccctttag gcctgataag acgcgccagc gtcgcatcag
   111241 gcatcgtgtg cgaatgtcgg atgcggcgaa aacgccttat ctgatctaca gacgtttaag
   111301 cttcgcaatt accggttcat tggctggcgg aaaatcatcg gcattaagac cgactagcga
   111361 catccactca ccgggttgcc cttctttacc ccacggctcc ccttcccagc tttcgaccag
   111421 ccagaaccac agagttatat gcctgtccgg gaattcatat tccagttttt caaatagcga
   111481 aaaatgttgt ggggtaatcc cgacttcttc ctgaagttca cgcaccaccg cctgttccgg
   111541 cgtttcaccc atttcaattt taccgccggg aaactccagt ttattcgcca tgtgcgcatc
   111601 tgctgcgcga cgcgttataa agatttcatt gttctcgttg cgaataatac ctaccgcaat
   111661 ttgcagcttt ttcattgtct taaacctata aaaaaggcgc agaatcctgc gccttttact
   111721 tcaacagtta gcttttattg caggcgacca tggcactgct tgtatttttt accggaaccg
   111781 cacgggcaag gatcgttacg tcctactttg cgttcaccgg tttgcgccgc cagtgcagct
   111841 gcggctgcag agtcgtcatc ctgatggcta agctgctgca tttgcgctaa acgctcggct
   111901 tccatacgac gctgttgttc cagctcctca acctcttcag gcatacgtac ctgaactttg
   111961 ctcagcgtac tgataacttc atatttcaac gactccagca tcgctgcaaa catggagaac
   112021 gattcacgtt tgtattcctg cttcggatct ttctgtgcat agccacgcag gtggataccc
   112081 tgacgcagat agtccatcgc tgccaggtgc tctttccaca gggagtcaag cgtttgcagc
   112141 atgacgcctt tctcgaagtg acgcatcatc tcagcaccaa ccacttcttc tttacgctga
   112201 tacacttcga tggactgcgc cagaatgcgc tcacgcagcg tctcttcatg cagttcgggt
   112261 tctttatcca gccactcagc aattggtaaa tcgaggtcga aatcgttctt cagacgttcc
   112321 tgcagccccg gaatatccca catttcttcc agcgactgtg gcggaatgta ggcatcaatg
   112381 gtggctttga acacatcttc acgaatgctg ttgatggttt cgctcacatc gctgacatcc
   112441 agcagttcgt tacgctggga gtaaatggcg cgacgctgat cgttagccac gtcatcatat
   112501 tccagcagtt gcttacgaat gtcgaagtta cgactttcaa ctttacgctg ggcgttggca
   112561 atcgctttag tcacccacgg gtgttcaatg gcttcgcctg gcttcatacc cagtttacgc
   112621 atcattccag atactcggtc ggaagcaaaa atacgcatca gcgcatcttc catcgacagg
   112681 tagaaacggg aagaaccagc atccccctga cgaccagaac gaccgcgcaa ctggttatcg
   112741 atacgacggg attcgtgacg ctcggtaccg ataatatgca ggccacctgc tgccagtacc
   112801 gcatcgtgac gtacctgcca gtcggcttta attttttcaa tttgctctgc ggtcggattt
   112861 tccagcgcgg caacttctgc ctgccagcta ccaccgagca caatatctgt accacgaccc
   112921 gccatgttgg tcgcgatagt caccgcagcc ggataacctg cctgagcaac aatcgccgct
   112981 tcgttggcgt ggaatttggc gttcaggaca ttgtgcttaa taccggcttt ggtcagttcg
   113041 tttgacacca gctccgattt ttcgatggag atagtaccca ccagcaccgg ctggcctttc
   113101 gcagtacgtt ctttgatatc ttcaatgatc gcctgaattt tttccgcttc agtcatgtag
   113161 accaggtccg gcagatcttt acgaatcatt ggacggttgg tcggaacaac gacggtatcc
   113221 agtttgtaga tggagctaaa ttcgaaagct tcggtatcag cagtaccggt catccccgcc
   113281 agtttttcat acagacggaa gtagttctgg aaggtgatcg aagccagcgt ttggttttcg
   113341 ttctggatct gcacaccttc tttcgcttcc acagcctggt gcagaccatc ggaccagcga
   113401 cggccctgca tggtacgacc ggtgtgttcg tcaacgatga taacttcacc atctttaacg
   113461 atgtagtcga cgtcacgggt aaacagcgca tgagcgcgca gcgccgccgt tacgtggtgc
   113521 atcagcatga tgttggccgg agagtacaga gactcccctt catccatgat gccctctttc
   113581 accagcagtt cttcaatcag caccagacca cgttcggtca ggttcacctg gcgagatttt
   113641 tcgtccaccg agaagtggcc ttcgccctgg aaggtttcgg agtcttcttt ttcctgacgg
   113701 atcaggtgcg gaataatttt attcacgcgt ttatacattt ccgagctgtc ttctgccggg
   113761 ccggaaatga tcagcggtgt acgcgcttca tcgatcagga tggagtccac ttcgtccacc
   113821 agcgcatagt gcagtttacg ctgtacacgt tcttcagggc tgaacgccat attgtcgcgc
   113881 aggtagtcaa agccgtattc gttgttcgta ccgtaagtga tgtcagctgc gtaagcttcg
   113941 cgctttgccg gtgctggcat gcccggcagg ttgataccga cagtcaggcc aaggaattca
   114001 aacagcggac ggttgttttc ggcgtcacgt tgcgccagat agtcgttgac ggtaactaca
   114061 tgcacgcctt taccggttag tgcgttcaga taagcaggca gcgttgcggt cagggtttta
   114121 ccttcaccgg tacgcatttc ggcgatgcag cgttcgttaa gaaccatacc gccgagtaac
   114181 tgaacgtcga agtgacgcat accaaagacg cgcttacttg cctcacgtac cacggcgaaa
   114241 gcttccggga tcagattttc cagcacttcg cctttttcca gacgtgcacg aaactctgcg
   114301 gttttccctt tcagttcttc gtcggagagt ttttccatct ccggttccat ggcattgatg
   114361 atgttgacca ctttgcgcat ccggcgcagg gtgcgatcgt tacgactacc gaaaacttta
   114421 gttaataatt tgattagcat aataaaatct caaacgcccc gcgttgcgga gttaataaaa
   114481 tgaagtaaag gtttattgtt gttagctgag gcgttgaggg ccagcacgga tgccttgcgc
   114541 ctggcttatc cagacgggcg tgctgaattt tgcttgtggg gtaaaatgcg cataatcaat
   114601 gcgataaccc ttttcagacg gcgtgccttc ctgggtcagc agcgcgctga gcgtatccag
   114661 taatgcaaga tgttgcgcct gaagaggcaa agattcttca gcaacgggca gcgtttgcgg
   114721 tgccattgcg aaggaaagat gacggattac cgtgcgaatg gcatgttgat gccagtaatc
   114781 aacggaatag ttcgaattcg ggcggcgcgt gttcgcttcc agcaaggcca attgaccaaa
   114841 gttaactttg gctgaaggct cgtggttgcg ggttgtcgct tttgcgggcg cgtttggttc
   114901 agcggcgttg ctgagcgcag gcaaacctaa actcgccgca accatcccta ataagagatg
   114961 cggccagaag tagcgtttac caaactgtcg ccagcgcgtc agtattccac tcacgttatt
   115021 gccatcccga tcgtattcaa acgtatttag cgcacaaatc ttatcattaa agcctgaagt
   115081 ccacagcacg aatgaagata agttgtgcgt aaaagtgctt aagaaaacag cgttcgcacc
   115141 gttttttata gctcttataa aattcaaaaa aaaacgactg gttcacctgg ccggagagag
   115201 tgccagattt gccagtcgaa ttttatacga cagtataaat gtcgttatgc cagtacagct
   115261 gaaggcgctt tgaaggccaa cggcagttct gcgtcgtcct ggaaggtcac atattcccag
   115321 gcttcctgtt tcgccaggac agcctgcagc agtttgttat tcagtgcatg accggattta
   115381 taagcggtaa atgcaccaat aatattgtga ccacacatga acaagtcacc gatcgcatcg
   115441 agcattttgt gacgcacaaa ttcgtcttca aaacgcaggc cgtcttcgtt cagtacgcga
   115501 taatcgtcaa caacgatggc acaatcgaag ctgccgccca ggcacaaacc acgggactgc
   115561 agatattcga tatcacgcat gaaaccgaac gtacgcgcac ggctgatctg gcgcataaac
   115621 gcatcagcgg agaagttcat cgcatagcgc tggttgctgg aatcaatagc cggatggtta
   115681 aaatcgatgg tgaaatccag cgaaaaacca ttgtacggct taaattcagc ccacttatcg
   115741 ccatcttcga cacgaacagt ctctttgatg cgaacaaatt tcttggcgca gttcaactcg
   115801 tcgataccgg cgtcaagcag caggtataca aacggagcgg cgctgccgtc catgatcggg
   115861 atttccggcg cgttaacttc gataacaatg ttatcgatgc ccaggcccgc gagagcagca
   115921 ttgaggtgct ctacggttga aatccgtaca tcatgctcgt tgaccagaca cgtacagagc
   115981 atggtatcac gcacagattt ggcatcggcc gggaaatcta ccggtggatt caagtcggtg
   116041 cgacgataga tgaccccggt gttggccggc gcagggcgta acgtcagggt gactttcttg
   116101 ccggtatgta aaccgacacc cgtcgcctga acgatacgtt taagtgtcct ttgtttgatc
   116161 atcgtattat ctcgccaaat tacctatcca accgaagtgt actatacatt cggcgggcca
   116221 gtttagcaca aagagcctcg aaacccaaat tccagtcaat tcttaatcag cttgcttacg
   116281 caggaatgct gggatatcca gataatccgg ctctttcgca gtttgcggcg cattgtcatt
   116341 cacgacttta gcaaccggct tctgctcctg ggtcagcgga gccatcccgt gctgctggta
   116401 gcgatccatc actggctgct gaacctgctt attggtcacc agagtgattt caggacgttt
   116461 gtccatgccg atacctgtcg caacaacggt tactcgcagc tcgtcattca tatccgggtc
   116521 aagagaagta ccgataacca cagtcgcgtt gtcggaagca aatgcacgga tggtgttacc
   116581 taccgtttcg aactcatcca gacgcaggtc gaagcccgcc gtgatgttaa ccagcacgcc
   116641 gcgcgcgcca gacaggtcga tatcttccag cagcggagaa gagatagcca tttcagcagc
   116701 ttcttccgca cggtcttcac cgctcgccac gccagaaccc atcattgcgt agcccatttc
   116761 agacattacg gtgcgtacgt ctgcaaagtc cacgttcatc aaacccggac gagtaatcag
   116821 ttcagcgata ccttgcacag cgcctttcag tacatcgttc gctgcgccaa acgcatccag
   116881 cagggagata ccgcggccca gaactttcag cagtttgtcg ttcgggatag tgatcagaga
   116941 gtccacatgc ttggacagtt cagtgatccc ctgctccgcg aatgccatac gcttcttgcc
   117001 ttcaaagttg aagggcttag tgacgacagc aacggtcagg atacccaaat cttttgccac
   117061 ttcagcgacg actggtgctg cacctgtacc ggtaccacca cccatacccg cagcaataaa
   117121 gaccatgtct gcaccttcca gcgccgcacg cagtgcatcg cgatcctcat cagccgcatt
   117181 gcggccaact tctggattag cgccagctcc cagtcctttg gtgataccgc taccgatttg
   117241 aatcgtctgt ccaaccgctg ttttacgcag cgcttgtgca tcggtattta ccgcgaagaa
   117301 ttcaacacct tcaatgcgct cgcgcaccat gtgttcaaca gcattaccgc cgccgccgcc
   117361 gacgccgatg actttaatca ccgcgtcatt ggtaagttcc attggttcaa acatagtttc
   117421 tctccgattt gtgcctgtcg cctgaggccg taatcattgt cggcctcata aaaattaaaa
   117481 ctcttttcgc agccaactat tgagtcgctt gatccacgag ccaactgatg ctgtaacacg
   117541 tttttctact tcagcttcac cgttaagatg tgactctttc ccatagtgaa gcaatcccac
   117601 cgccgtcgaa taatacggct cctgagcata atccgttaaa ccggtaatgt tcagcggcgc
   117661 gccgatacgc acttgcgtat gaaacacgcg ctgagcacag gctgcaagac cttcgatctg
   117721 cgctgcgcca ccagttaata caatgcctgc cgccaggtga tgtttaaccc cttgttggcg
   117781 aagcttttcc tgcaactgca atatctcttc gttgaccagg ttgagcaact cggtatagcg
   117841 cggctcgatc acctctgcca gggtctgacg ttgcagactc cgcggcggac gaccgcctac
   117901 gctcggcact tccacgctct catcttttcc aacgatggaa cccagcgcac aaccgtggcg
   117961 aactttaatc gcttcggcgt cgcttggcgg cgtgccaaag gcgtaagcga tatcactggt
   118021 cacgacattg ccagcataag gaattacctt agtgtggcgc aatgccccac cggtataaac
   118081 ggcgatatcc attgtaccac caccgatatc gacgacgcag acacccagtt cacgttcatc
   118141 ttccgtcaat accgaataac ttgatgccag accggcaaat atcagttggt caactttcag
   118201 cccacaacgt tcaaccgctt tgacgatgtt tttcgccata tcgttgtggc atgtgatcag
   118261 gtgcactttt gcctgcatcc gcacgcccga aagtcctacc ggattcttga tcccttcctg
   118321 atagtcaatc gcatactctt gcgggatcac atgcagcaca cgatgctcat cgcgcacacg
   118381 caccgatttc gcggtatgga cgacgttttc cacatcttct tgcgtcactt cttcttcaga
   118441 aataggcacc ataccaattt cattctggca gctgatgtgc ttaccagaaa gcgccagata
   118501 taccgaagag atctgacaat ctgccatcaa ttctgcctgg tcaatggcgc gttgtacgca
   118561 cttgaccacg gattcgaggt cgttcacccc gcctttatcc ataccacgcg acgggcagct
   118621 gcccacgcca atgatattga ccataccgtc gggcagaact tcccctacta aagcggcaac
   118681 cttcgcggta ccaatctcca gtcctactac cagttttctg tccgtcgcct tgatcattgt
   118741 tgttctgcct gtgcctgatt ttgttgctga gtagattcct ctggcggcaa gggcgcccag
   118801 cctactgccg ctccagagtc ataacgcaaa tcaacgtagc taatccgttt gccatcggtt
   118861 tgcgcctgct gctgtaaaac cggataaagt tctacaaagc gagccaaacg tttcatcgta
   118921 tcgccccggc caagattgag cttaatatcg ttattcagcg tcaactgcca ggaacgccgc
   118981 gcggtcatcg ccgcttcctt cagagtaaat ctgtcctttg ccagcatctg ccccatttcg
   119041 cgatagccct gcaacacttc attggcgctg ccttccgggc catacagcat tggaagcacc
   119101 tgcttgctgg tgcgatctgg cggcacgctg aaggtatttc cttccgcgtc taccatgtgt
   119161 tgatcattcc accgcgcaat cggcacatat tcaaccagat gaatcttcaa ttcatcaggc
   119221 cactgctttc tgacgctcac ctgcttaatc cacggcaggc gttgttctat ttgcgtctgg
   119281 atgatgttga catcctgggt cataaaggta cctggctcac ccaatgccag gatcgactgc
   119341 cggatatcgt cattacgcgt gtaatgccgt tcaccggtca acaccagctt tgagagcggc
   119401 aggcgttgcg catcttccat ccagcccaac acgacccagc cgctcaccaa cactgtcgtt
   119461 aaaacggtca gcaggaaaag gatccccgcc agacgcgttc cattattgcg gcgagaagaa
   119521 acctcttctt cgctgtttcg cgtgttcaga gcagcctgcg acatattagt ccgccagttc
   119581 cagaattcgt actaccaact gcgagaagct catacctgcc tgacgtgccg ccatcggcac
   119641 caggctgtgg ctggtcatac ccggtgaggt attggcttcc agcagataaa actgtccatc
   119701 gctgtccagc ataacgtcaa tacgtcccca tcctttgcaa cctaacgtcg tccatgcttt
   119761 cagcactaat gcctgcaaat tggcctcttg tgacgcttcc agacctgcgg ggcagaaata
   119821 ctgtgtctca tcagagagat acttcgcctc ataatcatag aaggttccgg ccggttgaat
   119881 acgtattgac ggtaaaattt cttcaccgag tatcgcaacc gtgaactccg gcccacttag
   119941 ccatttttca atcaatactt cttcatcgtg ctgaaatgcc aatcttaatg catcttgtag
   120001 agcattttct gctactactt ttgacattcc cacactggaa ccttcgcggc tcggcttaac
   120061 gataaccggc aaacccagag cagaaatttc tgctaactgc ttatcactca ggcctttttc
   120121 aaactctgcg cgggttaacg ctacccacgg cgcgaccggt aaaccggcac cttgccacag
   120181 aagtttgctg cgtagtttat ccattgaaag cgcagatgcc atcactccgc tgccggtata
   120241 aggcaagccc atcagctcga gcatgccctg cagcgtacca tcttcaccgc cgcgaccgtg
   120301 tagcgcgata aacactttct gaaagcccat cgacttcagt tgcgtcacgt cgacttcttt
   120361 cgggtcgaca ggatacgcgt caataccgcc ttcacgcagc ccggctaaca ccgctgcgcc
   120421 agaattcaga gaaacttccc gctcagcgga ggtcccgccc aacaggaccg cgattttatc
   120481 agtcatgttg ttcttcctcc ggagtttgcg gcttcagttt gatttcagct aaagaacggg
   120541 caatttttcc gatattacca gccccttgaa cgagaatcag gtcgttaccg gttaataccg
   120601 gtgccagcat ctcggctacc tgcgcagggt ccggcaccag aatgggatca attttcccac
   120661 gtccacgaat tgtgcgacac agcgaacggc tgtccgctcc cggaattggc gcttcgccag
   120721 ccggatacac ttccagcatc aacagggtat caacctgcgt cagcacattg gcgaaatcat
   120781 catacaggtc gcgcgtacgg gtaaaacggt gcggctgaaa cagcattacc aggtttttat
   120841 ccggccagcc tgcgcgcgcc gctttaatgg tggcgtcaac ttccgtcggg tggtggccgt
   120901 agtcatcgac cagcattgcc gtaccgcttt taccattcac tggctccagc gggaattcac
   120961 cgaggaaatc aaaacggcga ccagtcccct ggaagctctc cagcgcacgc aaaatagcct
   121021 cgtcgtcaat gccctcttcc gtagcaaccg caaccgcagc tgcggcgttc agcgcgttat
   121081 gacggcctgg cgcattcagg gtgacgcgca tcggctcttt gtcctggcgc agcagcgtaa
   121141 agtgcccctg cgggccaatc tgctgataat cttctacacg cacgtcggca tcttcgctga
   121201 agccgtaagt cgtggtctga cgccccacac gcggtaacaa ttcgcggatc accggatcat
   121261 caacacacat caccgcacga ccgtaaaacg gcaggttgtg cagaaaatta ataaaagtct
   121321 gttttaaatt ctcaaagtcg ccctggtagg tatccatgtg gtcggcttcg atattggtga
   121381 caatcgccac catcggttgc agatgcagga acgatgcatc actctcatct gcttcggcaa
   121441 tcaggtaccg accatgcccc aaacgcgcat gaacccccgc cgcttttacc agcccgccgt
   121501 taacgaaggt tgggtcgagc cccgcttctg cgtagatgct ggagaccatc gcggtggttg
   121561 tcgttttgcc gtgtgtaccg gcaatggcga tgccatgacg aaaacgcatt aactcagcca
   121621 gcatttcggc acgacggatc accggaatac gcgcttcatg agctgcgaca atttccgggt
   121681 tatcggctga aatcgcgctg gaaacaacga ccacgctggc gtcacgtacg ttttccgggc
   121741 gatggttgaa ataaatcgtc gccccaagat tcattaactg ctgcgtgacc ggatttggcg
   121801 ctaaatcgga accactgatc tgataacctt cattggccag aacttcggca ataccgccca
   121861 taccggcacc accgatgccg acaaaatgta tgtgccgaac gcgacgcatt tcgggcacga
   121921 tggaacgcag ttttgccaat tgttgtgtat tcattcttta cgccattaac ttcttaaatt
   121981 catacgatgc aaaaggcatc gctacaatta cgcccgggca gcccggctca cttcatttgc
   122041 cactcgctcg gtggcatccg gaatggatgc ggcgcgggcg cgttctgcca tggttaataa
   122101 ggtttctcgc gaccacccgg ccagggtgtt ggcgacagca tccacgctaa gctgtggctg
   122161 ctcgataatt ttggctgcgc ccgctttttc cagcggtagc gcattccagt attgctggcg
   122221 gtctttatgt tgaaacggca caaacaacgc cggtagtcct gccgcggcga tttcactcac
   122281 cgttaacgca ccggagcggc aaacgacgac atccgcccac gcatacgccg ccgccatatc
   122341 atcaataaat tcagtcactt tatgctgcgg ttgccccgct tcggcatacg cctgttcaac
   122401 ggattgttgc gaacctttgc cgctctgatg ccagatagtg actgaatcac ccagtttcgc
   122461 agcaacctgc ggcattgtct ggttaagaat gcgtgcgccc tgggaaccac ccactaccag
   122521 cacacgaacc ggaccttcac gtccagccaa acgttgctgc ggcaacggta gcgccaacac
   122581 atcggtacgc accgggttac ccaccacttc cgcattgggg aaagcacctg gaaacgcctg
   122641 catcactttg gtggcaatct tcgccagcca tttattggtt aagcccgcaa taccgttttg
   122701 ttcatgaagt acaaccggaa tgcctaacga ccacgcggcc agaccaccag gacctgatac
   122761 gtagccgccc ataccgagca ccacgtcagg tttgtacgct ttcataatcg cccgcgcctg
   122821 acgccaggca ttgaagatac gcagcggcgc agctatcagt gcttttatac cttttccacg
   122881 cagaccagag atacgaatga aatcaatttc gatgccatgt tttggcacta agtccgcttc
   122941 catacggtcg gcagtcccca gccagcgaac ttgccaaccc tgagccatta gatggtgcgc
   123001 aaccgccagt cccgggaata catgtccacc ggttccgcct gccatcacca ttaatcgctt
   123061 tccttgacca ctcatcgtga acctcgtaca aacgcctgcg ctttctccag acgcgtttca
   123121 taatcaatac gcaacagcat catgatggct gtcgacataa tcagtaagct cgaaccaccg
   123181 taactgatca gcggcaatgt cagacctttg gtcggtaaca tccccgccgc cgcgcctacg
   123241 ttaaccagcg cctggaagct aaaccagatg ccaatggaac aggcgagaaa accggaaaaa
   123301 cggtggtcaa tttctaacgc tttgcggcca atcgacatcg cacgaaaagc gacgaagaat
   123361 accattaaaa gtgccagcac cacaccgaca taccccagtt cttcgccgat aatggcgaaa
   123421 ataaagtcag tgtgcgcttc cggcagatac tccagttttt gtaccgagtt acctaaacct
   123481 tgcccccaaa gttcgccgcg accaaacgcc atcagcgatt gcgttaactg atagccgctg
   123541 ccaaagggat cttcccacgg gttccagaat gcggtaacac ggcggatacg gtacggttcg
   123601 gcgaggatca gcaacacaac cgctgaaatg cccataccga taatggcaat gaactgccac
   123661 aatttcgctc ccgccaggaa caacatcgcc agcgtagtca caaacaacac caccaccgta
   123721 ccaaggtctg gctgtgccag cagtaacact gccaacacca gaatcacgcc catcggtttc
   123781 aggaagccgc gcaggttatt acgtacttcg tcacctttac gcaccagata gttggcgata
   123841 tagcaaaaca gcgacagttt tgtcagctcc gcaggctgga tacgcagcaa accgagatcg
   123901 atccaacgcg atgccccttt aacagagcta cccactacca gcacgatcat cagcaggatg
   123961 atagatccga gcagcatcgt ggcactgtag cgttgccaga actccatcgg cagacgcagc
   124021 gtaatgatcg ccagaataaa cgccaaaatc agatagacac catcacgctt cgcgaagaag
   124081 aacggatcgt tggttaagcg ttgccctatg ggcattgacg ccgaggtcac catgataaag
   124141 ccaatcgccg cgaggccgaa ggtcagccac agtaaggtgc gatcgtacat gatcaggctg
   124201 tcggtatctt tttcccgcga gcccatcacc cagcccttta gcgccgtgga gatccagacc
   124261 aggatactga atcctggcag gcgcggcatt ttcaggcgag ggagagataa acgcatcaac
   124321 ctaactcctt cgccagacgg gcaaactcat tgcctcgttg ttcaaagttc ttgaactgat
   124381 caaggctggc acaggccggg gagagcagaa ccatatctcc tggttgaaca cgcggagcca
   124441 gcaagcgcat cgcctgttcc atagtttcgg tttgttctgc cacttccggg cgtaacgccg
   124501 ccagctgcgc gccgtcacga ccgaaacaat acagacgtac gttatcgcca ttcaggtaac
   124561 gcgccagtgg gctaaagtcc gccgatttac catcgccgcc cagcaacaaa tgcagcgtgc
   124621 cgtctacgtg caggccattc agcgccgctt ccgtactgcc gacgttggtc gcttttgaat
   124681 cgttaatcca acgtacgccg ttatgctcca gcacaacttc aaagcgatgc ggcagaccag
   124741 tgaatgtggt taacgctttc aggctgctgg ctcgcggtaa ccctgcggca tctgccagtg
   124801 ccagcgccgc cagcgcattg gtgtagttat gctgcccgga aattttcatt tctttcacgt
   124861 tcagcacttt ctcgcctttc acccgcagcc aggtttcgcc ctgctgatga ttcaggtgat
   124921 agtcacccat gttgacgcca aagctgacgc agcgttcatc cgcaccgcga atcggcattg
   124981 ttaaggcatc atcagcatta accacgcaaa ctttcgcgtt ttcgtaaatg cgcagttttg
   125041 ctgcacgata ctgttgtaaa ccaaacggat agcgatccat atgatcttca gtcacgttca
   125101 gaatggtcgc tgctaccgcc tgtaagctgg aggtggtttc cagctggaag ctcgacagtt
   125161 ccagtacgta cagttcgcac tcatcatcca gcagcatcaa cgcgggcagg ccaatattgc
   125221 cacccacacc aacgttaacc cccgccgctt tcgccatttc acccactagc gtggtgaccg
   125281 tgcttttgcc gttagaaccg gtaatcgcca caatcggtgc ttgtgcttcg cgacaaaaca
   125341 gctcgatatc gccaacgatt tcgattccgg catcagcggc agcgcttaag gatggatgcg
   125401 ccagtgcaat accgggactg gcgacaatca gatctgccgc catcagccat tcatcattca
   125461 gactgcccgt gtggcgttca acggcttcgg gtaatttatc caggccaggc ggtgtcatac
   125521 gcgtatccat aacgcgcggc gtcacaccgc gagcgaggaa aaagtccacg caggaaagcc
   125581 cggtgaggcc caggccgata atgacaacat ttttaccctg gtaatcagcc atgattaacg
   125641 taccttcagc gttgccagac caatcaggac cagcatcagc gaaataatcc agaaacgcac
   125701 aatgacgcgc ggttccggcc agcctttcag ttcatagtgg tgatgaatcg gtgccatgcg
   125761 gaaaatacgt tgtccgcgca gtttaaagga gccgacctgc aggatgacag aaagcgtttc
   125821 taccacgaac acgcccccca taatcaccag caggaattcc tgacgtagca gtacggcgat
   125881 aatgcctaac gcaccaccta acgccagcga acctacatcg cccataaaga cctgcgccgg
   125941 ataggtgtta aaccacagga agcccagtcc tgccccgact atcgcggtac agacaataac
   126001 cagttccccg gcgtgtcgca gatacggtat gtgtaaatag ctggcaaagt tcatgttgcc
   126061 ggtcgcccac gccaccagcg caaaaccacc ggcgacaaat acggtcggca taattgccag
   126121 accgtcaaga ccatcggtca ggtttaccgc gttgccagta cccacaatga cgaagtaagc
   126181 cagcagaatg tagaacagcc ccagttgcgg catcacatct ttaaagaatg ggaccaccag
   126241 ctgcgttgcg ggcgtgtctt tgccaacaag gtacagcgcg aaggcgacac ccagcgcaat
   126301 gaccgacatc cagaaatact tccaacgagc gatcaaccct ttggtgtctt tacgcactac
   126361 tttgcgataa tcatcaacaa agccaataac accgtaacct accagcacca ccaacacgca
   126421 ccagacgtac ggattggacg ggtaagccca cagcagtacg gagatcacaa tcgccgtcag
   126481 gatcataatc ccgcccatgg tcggcgtacc gcgcttgctg aagtgtgatt caggaccgtc
   126541 gttacgcacc acctgaccaa aggagagttt ttgcaaatga gcaatcatgc gcgggcccat
   126601 ccacaatgag atgaacagcg cggtcagcag gctgacgatg gcgcgaaacg tcagatagga
   126661 aaagacgtta aagccggaat aatatttgac caaatgttcg gccagccaaa ctaacatgtc
   126721 ccattctcct gtaaagcgcg tactacctct tccatggcgg cactacgtga acccttaact
   126781 aaaatcgtaa ttacctgttg ctcagcaatc agtgatttaa gacgcgtaat taacgcagtt
   126841 ttatcagcaa aatgttcgcc aacgccgctg gcggtgctga tagcatggct ttgtttaccc
   126901 acgcttaaca cgcggtcaat accagcagct tttgccgcct cgcccacctg tacatggcag
   126961 gcttcgcttt cagcgcccag ttccgccata tcgcccacca ccagcacgcg gtagcccggc
   127021 atttcagcca gtacctggac tgctgcggtc attgaaccga cattggcgtt gtaggagtcg
   127081 tcgagcagca actggttttc tgccagttgg atggggaaca gacggcctgg aacagctttc
   127141 agatttgcca gccccgcttt gatagcatca agcgttgcgc ccacggacat ggagagcgca
   127201 gcggctgcca gcgcattcgc aatattgtga cgccccggca acggcagcag aacatccacg
   127261 ctaccggttg gggtttgcag ggtaaattcc gtaccgtgcg aagtcacatg gatattggtg
   127321 gcggtgaaat cgctgttggc ggcattaggt gagaaacgcc acactttgcg tgagccaatt
   127381 acgctctgcc aattcagcca gtcgttgttg tcggcgttca taatggcgat accgttttcc
   127441 ggcaggccgc taaagatttc acctttcgct ttcgcgacac ccgcaagcga gccaaaacct
   127501 tccagatgcg ccgctgccag gttgttgacc agcgcagctt ccgggcgagt cagactcaca
   127561 gtccaggcaa tttcgccctg atggttcgcg ccaagttcaa taactgcgta atcgtattcc
   127621 ggcgttaagc gcaacagcgt catcggtaca ccgatgtcgt tgttgagatt gcctgccgta
   127681 taaagcgtgt tgccgcactg gcttaaaatc gccgccgtca tctctttaac ggaggttttg
   127741 ccggaggaac ctgtcagagc aaccacgcgc gccggaactt gctggcgaac ccatgcagcc
   127801 agttcaccaa acgccagacg cgtatccttg acgattaact gcggcaggtc gatgtccagc
   127861 ggacggctaa ccagtagtgc gcctgcgccg ccagctttcg cctggtcggc aaaatcatga
   127921 gcatcgaaac gttcgccttt cagggcaaca aacaggcagc ccggcgtcag ttttcgggta
   127981 tcagtggtta cagcatcaag ggtgatatct gcaccttgca gttcaccgtt gagaatgtcg
   128041 gtaagttggc taagggttac gctaatcatg caatcacccc cagcagacgc gccaccgtga
   128101 cgcgatcgga gtagtccaga cgctgattgc caacaatctg gtaatcttca tggcctttgc
   128161 ccgcgaccag taccacatca ttctctttag cctgcataac ggcgcaagtc accgcttcag
   128221 cacggccttc catcactttg gcatgtccgg catctaacat tcccgccaga atatcgttga
   128281 tgatggcacg cggttcttcg gtacgcgggt tatcgtccgt caccacagcc acgtcagcaa
   128341 actcttcagc aattgcgccc atcagtggac gtttgccttt atcacgatcg ccaccacaac
   128401 caaagacgca ccacagcttg cctgcacagt gaagacgtgc agcctgcaag gctttttcca
   128461 gtgcatccgg ggtatgcgcg taatccacaa ccaccgtcgg tttgcccggt gcagtgaaca
   128521 cttccatacg tccgcaaacc ggttgcagac gagcagcggt tttcagcaga tcagccagtg
   128581 gatagcccag cgccagcagc gtcgccagcg cgagcagcag gttgctgacg ttaaaagcgc
   128641 ccatcagatg gctttcaatt tcgccatcgc cccaacttga gctaaagcga atcgtcgcac
   128701 cgctgtcgtg atagttcacg tcgatcgctt ttaaccagcg tccgtgacag ttcggattga
   128761 tatgctcttc cattgatacc gcaaccgcgt ccggcagttt tgccagccag cggcggccca
   128821 cttcatcgtc ggcgttaata atcgcctgac cgcaatgatg ctcagaataa agcagccatt
   128881 tcgcggcttc gtagtgttcc atatcaccat gataatcaag gtgatcgcgg cttaagttgg
   128941 taaagaccga cgccgcaaat ttcaatgccg ccacacggtg ctgtaccagc ccgtgggagg
   129001 aaacttccat tgcgcaaaaa gtcgcgccct gatccaccag ccccgccagc tcgtgctgaa
   129061 catcgactgc ggaaccggta gtattttctg tcgggatcac tttcccaagc agaccgttac
   129121 caacggtgcc cattaccgcg ctggtttcgc caagcagttg gctccactgc gccagcagct
   129181 gggtcgtcgt ggttttgccg ttggtgcccg ttacgcccac gagacgtaaa ttgtcagagg
   129241 gttcatggta aaagcggccc gccagtgcag ataaacgctc gttgagctgg ctgagataga
   129301 tgaccggtac gccgtgcatt tcacggattt caccgtcggt cgcctcacct ttcgcctctg
   129361 caataatggc agccacacct tgcgctatcg cctgcgggat atatcgacgc ccgtccgcct
   129421 gatgacctac tacagctaca aagagatcgc ccgccgcagc cacacggctg tcgagtgtca
   129481 tctctcgcag tgctcgcgaa ggtgcgtctg gcacccacgg agcaagaagg tcgcgcaaat
   129541 tacgatctgc cacctgtccc ctcgccttga ttaatcacaa attcattttt atcgcccgtt
   129601 gtcagcgcat ccggctcgat gttcatggta cgcaatacgc cgcccatgat ggcaccaaag
   129661 accggcgcgg aaacggcgcc gccgtagtat ttacccgcct gcggatcgtt gataacaaca
   129721 accagcgcga agcgcggctg actcgcaggc gcaacgcctg cggtataagc aatatattta
   129781 ttgatgtagc gaccgtccgg cccgaccttt ttcgcggtac cggttttaat ggcgatacga
   129841 tagcctttaa tcgccgcctt cacgccgccg ccgcccggta gcgccacgct ttccatcata
   129901 tgcaccacgg tgcggacaat ggattccggg aagacacgtt caccgggaac cggggggtca
   129961 actttcgtaa tcgacagtgg gcgataaatg ccgtagctgc cgatagttgc gtagactcgc
   130021 gctaactgta acggtgttac cattagcccg tagccgaaag agaaggtggc cctctctatg
   130081 tcagaccacc gttgtttttg aggatataag ccactgcgtt ctccgaccaa ccccaaattg
   130141 gtcgcttttc ccagtccaaa acgtgagtaa gtatctacta acgctgagga cggcatcgct
   130201 aacgccagct tggaaacacc gacgttactc gacttctgta ataccccggt cagggttaat
   130261 tcgctgtagc gtgccacgtc tttgatttcg tggccgttaa ttcgataagg aatggtattg
   130321 agtaccgagt tttcccgcac cacgccacgt tgcaacgcag tcatcaccac catcggttta
   130381 accgttgagc ccggttcaaa cacgtcggtg atggtacggt tacgcatcgc ctctttcggc
   130441 gtgccactca gattgttagg gttgtatgat gggctgttag ccatcgccag cacttcaccg
   130501 gtgttgacat ccaccagcac ggcgctacca gattcagcct tgttaaaggc caccgcattg
   130561 ttcagttcgc gataaaccag cgcctgcagg cgttcatcaa tactcagcgc caggttgtgc
   130621 gcagcctggc tgtcagtaga agaaatatct tcaattacgc gaccatagcg gtctttacgc
   130681 acaatgcgct cacccggctg cccggtaagc catttatcga aactcttctc gacgccctca
   130741 atcccctggc tatcgacgtt agtaaagccg atgaggtgag cagtcacttc gccggatgga
   130801 taataacggc gagattcttc acgcagatga atccccggca gtttcagttt tttgatgtag
   130861 tccgccatgt cagggtttac ctgacgcgcc agataaataa agcgcccttt cgggttggcg
   130921 ttaatgcggg ctgaaagctg atccagcgga atattgagcg cgttagccag cgccttccag
   130981 cggtcaccga cgctgatacc gccagcgtca tgcacttctt tcgggtcagc ccaaatcgct
   131041 tttaccggca cgctcaccgc taacgggcga ccagaacggt cagtaatcat gccgcgggag
   131101 gtggaaactt gctgaacgcg aagagaacgc atgtcgccct ctttcaccag catatccggg
   131161 gagataactt gtaaccacgc tacgcgtccg agcagaaaag ccagcgccag gagaatacag
   131221 ccgcataaca acgcaaaacg ccaactgata aagttggcat gttcttcctg acgttttggt
   131281 ttctgcgttt tcgccgctgc tttcatgcgt cgcgtttatc cttatttttg cactacgata
   131341 ttttcttgtg acggatcaac atgctgcatt tgcagctttt ccgtggcgat cctttccacc
   131401 cggctatggt cgccgagcgc attctcttca aggatcaggt tgcgccattc aatgtctaaa
   131461 gcatctcgct ccagcaccag ttgttcgcgc tgagcggtca gtaaacgggt atggtgcgcc
   131521 gtggttacca cggtcaccgc cgtcaaaata atgcaaatga acaggcagag tggcagcttc
   131581 ccaaatcgca aaagatcgtc accgataaca ccaggcaatg catggcgctc gtggcttccc
   131641 atcgatcctt taactttgct tagagcttct gtcactctgc tgatcatgcg ttcgtcctct
   131701 ctgcaatacg cagaactgaa ctacgggcac gagggttctc agccacctct tcttcgcccg
   131761 gcattaactt gcctagtgct cgcagctgac ggccacccag ttttttgagc tgctcttcag
   131821 tcatcggtaa ccctgccgga acttgcggac cgcggctgtt ttcacgcata aaacgtttca
   131881 caatacggtc ttccagcgag tggaagctga tgatcgaaag ccgccccccc ggggccagca
   131941 cgttgagcga gctttttagc gcctgctcta tctcctccag ttcactgttt acccaaatgc
   132001 gcaccgcctg gaaggtacgg gtcgcgggat gtttaaactt atctttcacc ggcgttgcag
   132061 cagccacgac ttccgccagt tctttggtgc ggatcatcgg ctgttcgcgg ttacgctcga
   132121 caatggcgcg ggcaatgcgt ttggcaaaac gctcttcacc ataggttttc aatacccagg
   132181 cgatatcggc ttcttctgcg gtttgtagcc attcagcggc tgactgccca cgggttgggt
   132241 ccatacgcat gtccagcgga ccatcgcgca taaaggaaaa gccgcgttca gcatcatcaa
   132301 gttgcggtga agagacacca agatcgagga gaatgccgtc gatcttgccg ataagatcgc
   132361 gctcggcaac gtattcgccc agcgcggaga aaggtccgtg gatgatggag aagcgcggat
   132421 catcaatagt cttcgcaacg gcgatagcct gcgggtcgcg atcgatcgcc agcaaacgcc
   132481 cctcttcgcc aagctgcgag aggatcagac gtgagtgacc accgcgacca aaagtcccat
   132541 caatgtagat gccatcagga cggatattga ggccattaac ggcttcatcc aacagcaccg
   132601 tagtatgttt atagttttcc atcattttat agagacaagt cctgcagtcg ctccgataag
   132661 tctccggtag ccaactgctc tgcgtcgata tcttccttga cctgttgatg ccaggttgtt
   132721 tcatcccaca gctcaaactt gttgaactgt ccaaccagca tcacttcttt tgtcagcccg
   132781 gcatgttgcc gcagtactgg cgcgattaac aatcgacctg cgccatccat ctgacattcg
   132841 ctggcatgac ctaacagtag gcgctgcaca cggcgctcaa ccgggttcat gctcgacaga
   132901 cgcgataatt tttgctcgat aatttcccat tcaggcaggg ggtaaagcag caggcacggg
   132961 tgatgaatgt caatggtgca aaccatttga ccggcagcgt tctcaagcag ctgttcccga
   133021 taacgggtag gcactgataa gcgccctttg ctgtcgagat tgactaacgt tgctccccgg
   133081 aacatgccag cctcacccct tattcccact ttgccccaca atttcccact gaaaggagtt
   133141 tacggagcgg aggaaaagct tgtcaagcca gtcacaaggc ataccgaggt aaaaaacccg
   133201 atgtttacgg ctaatatcag cgttgtttat tttacgtcca acgtgcgaag caaattaacg
   133261 ttatgaatag ctgtaagaaa aaaaccctca tcgtcatcac cgttttaaaa tgactcacta
   133321 ttttccacag caaatataaa gtgtcagttt gcgacgcgag cggcatttta ggacatatct
   133381 tccccggtta acagtccctg ttgcgtctgt gtagcgcttg atgccagcgg gtttgcgcaa
   133441 aagcaaagaa aagtgcttgt taaatcggca attcatctct gcatgtaaca aaataatgca
   133501 aaaacgcgct gtcgatatca cttaattatt gtaaatttca gctggtgagt taattaatgg
   133561 gcgggtttaa aagcatttat caataatagt caggaatagt cttatttact ttaaggatat
   133621 tgatgtccag tcccgtactc tacgcgccag agtgaaattc acctggcgcg tatttttgtt
   133681 cgcagcttag ctacggctga gcacgccgcg gcgatagaga ttacgtttaa tgcgcgttaa
   133741 accaggtttt ggcttacgtg gttcgtccag gcttgccagg acaatctcca gcacacgctc
   133801 tgcgacatcg cggtgacgtt gagccactgc cagcaccggg cactgtaaga agtcgagcag
   133861 ttcgttatcg ccaaaggtgg caattgccag gtcagaaggc agtttaccat cgcgacgtag
   133921 cgtgacatcc atcacccctt gcaacaacgc aaacgacgtt gtgaacagcg cctgcggcat
   133981 cggatgcgtt tccagccatt tttcgaataa ctgggcagcc gcctcccgct catagctgtt
   134041 ggcatacaga aaatgcactt cgcgcggatc atctttccag gcagtacgga aaccttgttc
   134101 acgcaggaag ctgacagaaa gctccggcag cgcgccaaga taaagcaccg tctcggcggg
   134161 aaacttacgt aactcttccg ccagcatttc ggcatcatcc tgatcggcac caaccacgct
   134221 ggtaaagtgt tcacgatcga gggcgcggtc aagcgcgaca atcgggaacg ggtcgttagc
   134281 ccagcgttga taaaaaggat gctcaggagg caacgacgtc gaaacaataa tggcatcgac
   134341 ctgacgctgt aaaaggtgct caatacagcg catttcgttg tctggctgat cttctgagca
   134401 ggcaatcagc agttgatacc cccgttgccg cgcctggcgt tcaagatagt tagcgatgcg
   134461 ggtatagctg gtgttctcca gatcggggat cacaagacca atagaacgtg tgcgtccagc
   134521 acgaagccca gctgccacgg cgttcgggtg gtaattgtgc tcacgcacga cagccatgac
   134581 tttttcaacg gttttgtcgc tcacacggta ttgcttcgct ttgccgttaa taacatagct
   134641 tgcagtggtc cgcgacactc ccgccagccg agcgatttca tccagtttca caattgcccc
   134701 ttgcgtaaaa tgtaaaaacc atagccattg tactggtatg ggttaaattc attaacatct
   134761 aagcgcagaa aattaactgc ggcaactgct tttattccag gttcccactg atttcgcaaa
   134821 aaaagcccaa cgtgacatgt tgggctgtaa attgcgcatt gagatcattc tcaacgcatt
   134881 attttatcgc cgcgcgaaag tccgaccaca ccagagcgag caacttccac aattttcgcc
   134941 acatcgcgaa tcgatgctaa aaatgcatca agcttatcgc tggtgcctgc taattgaacg
   135001 gtataaagcg agggtgtgac atcaataatt tgcccacgga atatttccgt attacgtttc
   135061 acttcgtcac gcccgtaacc gctggcctga attttcacca gcatgatttc ccgctcaaca
   135121 tgcgcgccct gccccaactc actcacgcgc aagacatcga ccagcttgtg taattgcttt
   135181 tcgatctgct caagtacttt ttcatcgccc acggtctgga tggtcatacg cgataatgtc
   135241 ggatcgtcgg ttggcgcaac ggtcaggctt tcaatgttgt agccacgctg ggaaaaaaga
   135301 ccaatcacgc gggataacgc gcctgattca ttttcgagta agactgataa tatccggcgc
   135361 ataatcaggt tctctccgtt ttgcttaacc acatttcatc cattccgccc ccgcgaatct
   135421 gcatcgggta gacgtgctcg ctgccatcga cggtaacatc aacaaacacc aggcgattat
   135481 tgcgcacctg ttccagcgcc tcgctaagtt tgctttccag ctcatgcgga tgagaaatct
   135541 ggatcccgac atgcccatag gcttccgcca gacggacgaa atcgggtagc gattgcatat
   135601 aagattgtga atgacggccg gaatagatca tgtcctgcca ctgcttcacc atccccagat
   135661 agcggttatt gagattcacc accagtacgg gcaactcgta ttgcaacgcg gtagacagtt
   135721 cctgaatgtt catctgaata ctgccgtcgc cagtgacgca aaccacggtt tcttctggca
   135781 acgccatttt gacgcccagt gccgcaggta aaccaaaacc catcgtgccg aggccaccgg
   135841 aattgatcca gcgacgcggt ttgtcgaatg gataataaag tgcagcaaac atctggtgct
   135901 gcccgacatc ggacgtcacg taagcgtctc ccttcgtcaa ccgccaaaga gtctcgatca
   135961 ccgcctgcgg tttaatcttt tcactgtgag tgtcatattt caggcactga cgagcgcgcc
   136021 actgttcaat ttgctgccac cagtcgcgga tctcatccag tggttgatgg gcggattctt
   136081 gcgacaagag ttcaagcatt tgttcgagga cctggcgagc atcccccaca atcgggatat
   136141 ccgcagtcac ggttttagaa atggaagtag gatcaatatc gatatgcaga acagtggcat
   136201 ttgggcagta ctttgccaga ttgttcgtcg ttcggtcatc aaatcgtacc ccgacggcga
   136261 aaatcacatc cgcgttatgc atcgtcatat tggcttcgta ggtaccgtgc attcccagca
   136321 tgcccagtgc ctgacgatgc gttgccggaa acgcccccag ccccatcaat gaggaaacaa
   136381 cgggcagatt caacgcctcc accgtttctt tcaactgctg atggcagccc gccgtgattg
   136441 ccccaccgcc tacgtagaca accggttttt ttgccgctac cagcgtttgc agagcacgct
   136501 taatttgccc tttatgtccg gtagtagtgg gattgtaaga acgcatactg accgactccg
   136561 gccagacata gggtaatttg ttcgccggat taagaatatc tttcggtaaa tcaacgacca
   136621 ctggtcctgg gcgaccactt gccgccagcc agaaagcctt tttcagcacc tgcggaatgt
   136681 cttccgtttg cttaaccaga aaactgtgtt taaccaccgg tcgcgaaatc cccaccatgt
   136741 cgcactcctg aaaggcatcg taacctatca acgaggtcgc tacctgcccg gaaaggacaa
   136801 ctaatggaat ggaatccata taagcggtgg cgatgccagt aatcgcattg gtcgcccctg
   136861 gacccgacgt taccagcacg acgccgactt ccccggtcgc gcgcgccaga ccatcggcca
   136921 tatgcactgc cgcctgctca tgacgaacta aaacatgatc gataccaccc acggtatgca
   136981 atgcatcgta aatatcaagg actgcgcctc cgggataacc gaatacttgt ttaacgccct
   137041 gatcgataag cgatcggacg accatctcgg ctccagacaa catctccatg gcctgcctca
   137101 ctgtttgacg gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg
   137161 cagccagaga cataaacgcc gggtaataag aataaacagg aggaaaggtg aaaagaaacg
   137221 ataaaatcct ccattacgaa ggataaaaat gataataaat cggctaaatc ccaccactta
   137281 cagcaatttc tctatttttt taattaatcc taaaatagag agcccacaca ttcagaataa
   137341 aattctacat tcaataaaaa atgcagaata aaccagacat tcatgtctga cctattctgc
   137401 attcagttag cgtttgcaaa ttgagactaa ttgctcttcc atccactgat ggcctttatc
   137461 gcgcccggca gcttcatgcc aggagagata acaggttctg ctatttagtt ttaacggcag
   137521 cggtaatacc tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat
   137581 agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata
   137641 cgcgatactg gcttgtttat caaccgtgtc ataccacggt tgactaaatg acgcgaaacg
   137701 atcgagcgaa accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccctt
   137761 aattgttggg tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct
   137821 ggtaaattca ggacgatgga agtcttcata actaatcaca aactccgttt cctgataacg
   137881 cagctgatgt tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc
   137941 aatctgctca atgtgattat aaatctgcga ggtcagaata ctgtctaacg ggctgcaaac
   138001 acaaagatga aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt
   138061 ttgtactagt tgcaatgcct gacgaactga accaaaaagt tgaaatgcgc gagcagtcgg
   138121 ttgaatacca cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc
   138181 aacagcgtta ctgaccgcag gttgcgacat tcccagaaca tgagcagcac gggtaatgtt
   138241 ttgctcctgc atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg
   138301 tggcttgctg aactccgcca tctctggatg atctgtttgt acctctggca tacttaactc
   138361 cactgtcaca cttaactccc tttcccttat tggaatgcag agaaataatc ctcgagaata
   138421 tgatttatca tgcatataaa ataagaaaaa ggaaaatgag taaaattagg aaaacataaa
   138481 gatacgggtt cttataaaaa cagaatccat tgccgtggaa agcgtcactt accacagcat
   138541 aataatccat aatgtttttg ttgcgaaaac aatctaatca taactaccgc ga
//
LOCUS       DAZFFG010000002       120188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFFG010000002 DAZFFG010000000
VERSION     DAZFFG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 120188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 120188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.002"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            111..1232
                     /locus_tag="ACHJ7J_000128"
     CDS             111..1232
                     /locus_tag="ACHJ7J_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4998414.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTIYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLEMGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            1232..4339
                     /locus_tag="ACHJ7J_000129"
     CDS             1232..4339
                     /locus_tag="ACHJ7J_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO4998415.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYLVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            4343..5716
                     /locus_tag="ACHJ7J_000130"
     CDS             4343..5716
                     /locus_tag="ACHJ7J_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO4998416.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDHRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            5725..6888
                     /locus_tag="ACHJ7J_000131"
     CDS             5725..6888
                     /locus_tag="ACHJ7J_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO4998417.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAISCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(6939..7745)
                     /gene="sapF"
                     /locus_tag="ACHJ7J_000132"
     CDS             complement(6939..7745)
                     /gene="sapF"
                     /locus_tag="ACHJ7J_000132"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO4998418.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(7747..8739)
                     /gene="sapD"
                     /locus_tag="ACHJ7J_000133"
     CDS             complement(7747..8739)
                     /gene="sapD"
                     /locus_tag="ACHJ7J_000133"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO4998419.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(8739..9629)
                     /gene="sapC"
                     /locus_tag="ACHJ7J_000134"
     CDS             complement(8739..9629)
                     /gene="sapC"
                     /locus_tag="ACHJ7J_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO4998420.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(9616..10581)
                     /gene="sapB"
                     /locus_tag="ACHJ7J_000135"
     CDS             complement(9616..10581)
                     /gene="sapB"
                     /locus_tag="ACHJ7J_000135"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO4998421.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(10578..12221)
                     /gene="sapA"
                     /locus_tag="ACHJ7J_000136"
     CDS             complement(10578..12221)
                     /gene="sapA"
                     /locus_tag="ACHJ7J_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO4998422.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(12534..12779)
                     /gene="ymjA"
                     /locus_tag="ACHJ7J_000137"
     CDS             complement(12534..12779)
                     /gene="ymjA"
                     /locus_tag="ACHJ7J_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO4998423.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(12913..14298)
                     /gene="puuP"
                     /locus_tag="ACHJ7J_000138"
     CDS             complement(12913..14298)
                     /gene="puuP"
                     /locus_tag="ACHJ7J_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO4998424.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            complement(14288..14452)
                     /locus_tag="ACHJ7J_000139"
     CDS             complement(14288..14452)
                     /locus_tag="ACHJ7J_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998425.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(14601..16019)
                     /gene="puuA"
                     /locus_tag="ACHJ7J_000140"
     CDS             complement(14601..16019)
                     /gene="puuA"
                     /locus_tag="ACHJ7J_000140"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO4998426.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQDGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            16231..16995
                     /gene="puuD"
                     /locus_tag="ACHJ7J_000141"
     CDS             16231..16995
                     /gene="puuD"
                     /locus_tag="ACHJ7J_000141"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO4998427.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            17022..17579
                     /gene="puuR"
                     /locus_tag="ACHJ7J_000142"
     CDS             17022..17579
                     /gene="puuR"
                     /locus_tag="ACHJ7J_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO4998428.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            17729..19216
                     /gene="puuC"
                     /locus_tag="ACHJ7J_000143"
     CDS             17729..19216
                     /gene="puuC"
                     /locus_tag="ACHJ7J_000143"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO4998429.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            19218..20498
                     /gene="puuB"
                     /locus_tag="ACHJ7J_000144"
     CDS             19218..20498
                     /gene="puuB"
                     /locus_tag="ACHJ7J_000144"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO4998430.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            20536..21801
                     /gene="puuE"
                     /locus_tag="ACHJ7J_000145"
     CDS             20536..21801
                     /gene="puuE"
                     /locus_tag="ACHJ7J_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO4998431.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVAYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(21921..22898)
                     /gene="pspF"
                     /locus_tag="ACHJ7J_000146"
     CDS             complement(21921..22898)
                     /gene="pspF"
                     /locus_tag="ACHJ7J_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO4998432.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSDNTSLPMLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            23065..23733
                     /gene="pspA"
                     /locus_tag="ACHJ7J_000147"
     CDS             23065..23733
                     /gene="pspA"
                     /locus_tag="ACHJ7J_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO4998433.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            23787..24011
                     /gene="pspB"
                     /locus_tag="ACHJ7J_000148"
     CDS             23787..24011
                     /gene="pspB"
                     /locus_tag="ACHJ7J_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO4998434.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            24011..24370
                     /gene="pspC"
                     /locus_tag="ACHJ7J_000149"
     CDS             24011..24370
                     /gene="pspC"
                     /locus_tag="ACHJ7J_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO4998435.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            24379..24600
                     /gene="pspD"
                     /locus_tag="ACHJ7J_000150"
     CDS             24379..24600
                     /gene="pspD"
                     /locus_tag="ACHJ7J_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO4998436.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            24675..24989
                     /gene="pspE"
                     /locus_tag="ACHJ7J_000151"
     CDS             24675..24989
                     /gene="pspE"
                     /locus_tag="ACHJ7J_000151"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO4998437.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            25108..26505
                     /gene="ycjX"
                     /locus_tag="ACHJ7J_000152"
     CDS             25108..26505
                     /gene="ycjX"
                     /locus_tag="ACHJ7J_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO4998438.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            26502..27563
                     /gene="ycjF"
                     /locus_tag="ACHJ7J_000153"
     CDS             26502..27563
                     /gene="ycjF"
                     /locus_tag="ACHJ7J_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO4998439.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            27711..29252
                     /gene="tyrR"
                     /locus_tag="ACHJ7J_000154"
     CDS             27711..29252
                     /gene="tyrR"
                     /locus_tag="ACHJ7J_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO4998440.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(29296..29802)
                     /gene="tpx"
                     /locus_tag="ACHJ7J_000155"
     CDS             complement(29296..29802)
                     /gene="tpx"
                     /locus_tag="ACHJ7J_000155"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO4998441.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVNFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            29921..30886
                     /gene="ycjG"
                     /locus_tag="ACHJ7J_000156"
     CDS             29921..30886
                     /gene="ycjG"
                     /locus_tag="ACHJ7J_000156"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO4998442.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQQILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(30861..31589)
                     /gene="mpaA"
                     /locus_tag="ACHJ7J_000157"
     CDS             complement(30861..31589)
                     /gene="mpaA"
                     /locus_tag="ACHJ7J_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO4998443.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(31880..32518)
                     /locus_tag="ACHJ7J_000158"
     CDS             complement(31880..32518)
                     /locus_tag="ACHJ7J_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4998444.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIDENIGINQPGTDGDKPFFM"
     gene            complement(32539..33468)
                     /locus_tag="ACHJ7J_000159"
     CDS             complement(32539..33468)
                     /locus_tag="ACHJ7J_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4998445.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAVISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHL"
     gene            complement(33468..34388)
                     /locus_tag="ACHJ7J_000160"
     CDS             complement(33468..34388)
                     /locus_tag="ACHJ7J_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4998446.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            34526..35425
                     /gene="pgrR"
                     /locus_tag="ACHJ7J_000161"
     CDS             34526..35425
                     /gene="pgrR"
                     /locus_tag="ACHJ7J_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO4998447.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            35762..37375
                     /gene="mppA"
                     /locus_tag="ACHJ7J_000162"
     CDS             35762..37375
                     /gene="mppA"
                     /locus_tag="ACHJ7J_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO4998448.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTNYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(37426..38457)
                     /gene="ynaI"
                     /locus_tag="ACHJ7J_000163"
     CDS             complement(37426..38457)
                     /gene="ynaI"
                     /locus_tag="ACHJ7J_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO4998449.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            38701..38958
                     /gene="ynaJ"
                     /locus_tag="ACHJ7J_000164"
     CDS             38701..38958
                     /gene="ynaJ"
                     /locus_tag="ACHJ7J_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO4998450.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(39008..39958)
                     /gene="uspE"
                     /locus_tag="ACHJ7J_000165"
     CDS             complement(39008..39958)
                     /gene="uspE"
                     /locus_tag="ACHJ7J_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO4998451.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(40110..40862)
                     /gene="fnr"
                     /locus_tag="ACHJ7J_000166"
     CDS             complement(40110..40862)
                     /gene="fnr"
                     /locus_tag="ACHJ7J_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO4998452.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(41057..41572)
                     /gene="ogt"
                     /locus_tag="ACHJ7J_000167"
     CDS             complement(41057..41572)
                     /gene="ogt"
                     /locus_tag="ACHJ7J_000167"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO4998453.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(41583..43109)
                     /gene="abgT"
                     /locus_tag="ACHJ7J_000168"
     CDS             complement(41583..43109)
                     /gene="abgT"
                     /locus_tag="ACHJ7J_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO4998454.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(43146..44591)
                     /gene="abgB"
                     /locus_tag="ACHJ7J_000169"
     CDS             complement(43146..44591)
                     /gene="abgB"
                     /locus_tag="ACHJ7J_000169"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO4998455.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETKVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(44591..45901)
                     /gene="abgA"
                     /locus_tag="ACHJ7J_000170"
     CDS             complement(44591..45901)
                     /gene="abgA"
                     /locus_tag="ACHJ7J_000170"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO4998456.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGIIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            46077..46985
                     /gene="abgR"
                     /locus_tag="ACHJ7J_000171"
     CDS             46077..46985
                     /gene="abgR"
                     /locus_tag="ACHJ7J_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4998457.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            47315..47878
                     /gene="smrA"
                     /locus_tag="ACHJ7J_000172"
     CDS             47315..47878
                     /gene="smrA"
                     /locus_tag="ACHJ7J_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO4998458.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(47899..49095)
                     /gene="dgcM"
                     /locus_tag="ACHJ7J_000173"
     CDS             complement(47899..49095)
                     /gene="dgcM"
                     /locus_tag="ACHJ7J_000173"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014832102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO4998459.1"
                     /translation="MVTVWIVSPFEEQLIYANSAARLLMQDLTFSQLRTGPYSVSSQK
                     ELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVIIFEGIEAPATLG
                     LKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAALNFYGYNHETMC
                     QKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQTYAGPIEIYGDK
                     LMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLL
                     LIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLD
                     TALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRVDDALYRAKNDGR
                     NRVLAA"
     gene            49266..50480
                     /locus_tag="ACHJ7J_000174"
     CDS             49266..50480
                     /locus_tag="ACHJ7J_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4998460.1"
                     /translation="MLTDTKLRAIANKPYDGPNEIADRDGLSARISPKGKIIFQFRYR
                     FNGKPVRMKIGDYGSMTLKDARYAVAEYKELLAKGKNPTVSVKQAISIERGKLTISAI
                     VSDYLSMPNVIKLVAYNDVRSALKRHVVDKFGEYIADDVSTKQWMDIFGEITAAGHAV
                     TAGLILKRMKIVVNSAIRRGLMTSTAINNIRVIDVGENYAVGERYLSCDEIGHFWRTV
                     DDSQIYRQNKIALKLLLLTGCRVGELQKMRREHLDLDNGVWTVPAAVAKTRSEIRRGL
                     SDLSIEMLREVLASHEYDYVFPPVINGGNRPVNASVIKVAAIFVRKRMGGKSWSCHDL
                     RRTCRTHLSAIGVPVHIAEKILGHSLRGILAVYDKYDYLDEQKEALNKFADYVLSLAD
                     VKPISSKNRITS"
     gene            complement(50420..50650)
                     /locus_tag="ACHJ7J_000175"
     CDS             complement(50420..50650)
                     /locus_tag="ACHJ7J_000175"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4998461.1"
                     /translation="MDKTATILLSRPAICRMLGGISRGTFYSWRKKWEENGTPFPDPV
                     DVLGTGRGVMYRYQDVMRFFDEIGLTSAKDNT"
     gene            complement(50650..51312)
                     /locus_tag="ACHJ7J_000176"
     CDS             complement(50650..51312)
                     /locus_tag="ACHJ7J_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998462.1"
                     /translation="MNKLKAIGVISHRTNPECYPSFEVTTCRTEYNFGSYYLLGVRAD
                     TGGTYSVMAASWKFDKYANLSNKDDDGMNKESEIIDELIEDERHDCETQPEKTEWAVG
                     EKPPCGVWLELLDPVDGRKYVILKYKYISYRLALFVEMDAPDFEQAGEWKHFAHKLYV
                     DPKEKALAEIAFSLAVNVMGEDAAKEIDFNHDNDFSCDYRNMAQAIIDGCIAHVEYTG
                     NK"
     gene            complement(51327..51503)
                     /locus_tag="ACHJ7J_000177"
     CDS             complement(51327..51503)
                     /locus_tag="ACHJ7J_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998463.1"
                     /translation="MVRYYMAKPTGILYKVDGEYVYYFHNHAREWRLCHAHFKQEIEN
                     HPEYFIKVDNVTVA"
     gene            complement(51503..51934)
                     /locus_tag="ACHJ7J_000178"
     CDS             complement(51503..51934)
                     /locus_tag="ACHJ7J_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO4998464.1"
                     /translation="MRQIRFEIVNDAVKENAIRQIREIQPDSKSPLIITIQEKTRSLS
                     QNALLWALLTDVSDQVNWYGKKLSPEDWKAVFTAGLKKYGVVPNLDKSGFVVLGTSTS
                     RMSKAEFSELIELIYSFGAEHGVQWSGDTKLNEEFIKRWGR"
     gene            complement(51931..52128)
                     /locus_tag="ACHJ7J_000179"
     CDS             complement(51931..52128)
                     /locus_tag="ACHJ7J_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998465.1"
                     /translation="MNRELDLTVEDLSTIAEYMRGDDPDKPVVVDMRYLKSAFMTSSR
                     LISLRAIMYARARWKNSNGVS"
     gene            complement(52342..52782)
                     /locus_tag="ACHJ7J_000180"
     CDS             complement(52342..52782)
                     /locus_tag="ACHJ7J_000180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998466.1"
                     /translation="MPETIKHLIKNGIVERVYTCFGRRRRLLGYRIKQLYAERRERVA
                     SLFGDYSVKKRMRDISAETGIPWNYLSRTLRLMVIDETLCVDPNRHGVNFYSLFKPGR
                     FGHANDLAFDFDRRLNEYRKNNGLLPDKPIFEIEKLNGETGLEL"
     gene            complement(52979..54394)
                     /locus_tag="ACHJ7J_000181"
     CDS             complement(52979..54394)
                     /locus_tag="ACHJ7J_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4998467.1"
                     /translation="MVTEQINSLRQEREASVIGGLLLGGLTPNAQDVLATLDPEVFTI
                     PLYKRAFEIIRAQARNRNLIDALLVGDEIGNENFVPLMQTARSCPSAANLKGYAQLLR
                     EEYQRRQMLELIDDMRYKLETGTLEVVKETMKDFDSRYSKLKVTKDKIIPVLLRDAVQ
                     EYTEVLSKRMECGVNSDNIKTGIEPLDEMLGGINATDLVLLAGRPGSGKSALALAIAR
                     AAAERPYPGSKDQRVGVLFFTLEMSLDQMTERAIAGAGNLSTDCLRNPVKLDDEGWAH
                     VGQGMSALANLDVWIVDASQLTVEEIRATTERMKQEHPNLGMVMIDYIGLMKLAKAER
                     HDLAVGQLSWSLKMMAKELRVPVLALAQLSRRVEERPNKRPNNSDLRDSGNLEQDADR
                     IIMVYRDGYYDEQSVAREYMEIIVSKNRHGKSGTVYQRFDDNGNILPCDQARAAAACI
                     QSMNHRTSRFNQRNNQNNTSF"
     gene            complement(54394..54936)
                     /locus_tag="ACHJ7J_000182"
     CDS             complement(54394..54936)
                     /locus_tag="ACHJ7J_000182"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO4998468.1"
                     /translation="MMWDDLFTYRDGVLYWSCNRANNKIKAGSVAGSKHHRGYWTICV
                     NGICCTWRHRIIWEMFNGPIPDGMEIDHINHIPGDDRIENLRMVTRQENNRNKIMPAN
                     NTSGCVGVTWNKKTHKWRAQIGIRGSDGKKKTIYVYYGDSFDDAVKARKEAERIYNFH
                     SNHGISLPIENTFCKQEVMT"
     gene            complement(54923..56053)
                     /locus_tag="ACHJ7J_000183"
     CDS             complement(54923..56053)
                     /locus_tag="ACHJ7J_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998469.1"
                     /translation="MSQRKISDEQLIAEYNNGLTYKQIAEKYGMSKRNVERLGAKLAK
                     RGLLSTRRAPGFGVNGESLLVDKNGNVIMRWIKTARDRDEMERLMEAARDAFTEEIPR
                     AEAVPVPEIDFQKSLALYPVFDLHIGALAHKAECGESYDTGIAERVLNDFFDYAVGAA
                     PMSEKAVLLLGGDVLHTDGLLPVTPSSNHVLDCDSRYAKLVYVAIRSVRRAVGKMLLN
                     HKDVEIQVLSGNHDQSGMIWLRAALAAFYEDEPRVTVDVSPTIVHHTQYGKTFLAYHH
                     GHTIKKPENLLSACVADWREDFGKSKYVYVHVGHLHHKSVTESSIGIVEHHGTLAGKD
                     AYSTNGGWRSQRCAAVIIYSPDYGEIGRFVYRPEMVGCHDVG"
     gene            complement(56087..56998)
                     /locus_tag="ACHJ7J_000184"
     CDS             complement(56087..56998)
                     /locus_tag="ACHJ7J_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO4998470.1"
                     /translation="MRKLTHEEQVAVIADVNPDVEVIGEITGNKTKVTCRCKVCRHEW
                     SPTPCNLKRGQGCPKCAGNAKLTHEEQVVAIAKVNPDVEVTGEITGDKTKVLCRCKVC
                     RHEWSSIPNALKCGHGCPKCARLKATLTHKEQVANIVKANHDVEVLGKITGDAKKVLC
                     RCKVCNHKWLAKPSHLKCGHGCPKCGKRGFLSHDHGKLYIMVDDLEVPTQMKIGVSVK
                     ENERRNQVLRSAHKAGVIITDLHIVKTLYGTTENMYELERTMHKAFSNYKINFPVKFS
                     GSEEFFYYRPEVFDMVEEAYKEIICCQ"
     gene            complement(57373..58359)
                     /locus_tag="ACHJ7J_000185"
     CDS             complement(57373..58359)
                     /locus_tag="ACHJ7J_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4998471.1"
                     /translation="MSMRLMVEVFHTEVGNPMHKLVLLKLADNANDNGECYPSYQHIA
                     KQCEINKSTAMRAIEALCKKGLLLKQPRYLIGGKEQTSNFYVIVLPSLSGSGTKQPGV
                     VAESNQGSGTKQPGVVAESNPNQSYITSKENIKDIPPCIPQEEKHVNQDGVNEAALRC
                     LDFYNDKAGCKYRDAKPFIELLTETKTRKAYTEDEITLVIEWALTQWRNRGGTPKPIN
                     ICRVTKFDGYLADAEQWRRVTATVNAAVVVSAFNQTFDGLLPPAELDRDLERKIYAFT
                     DYLKDKSITGFIAYFEAFKNTASDFYFGNGFTATLDFLLKPKTLRDTRAGAL"
     gene            complement(58381..58626)
                     /locus_tag="ACHJ7J_000186"
     CDS             complement(58381..58626)
                     /locus_tag="ACHJ7J_000186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998472.1"
                     /translation="MYYVRKAFDIRLNNNDPTQKLILIALATKAGCNGKSEVTPEEIA
                     GMCEIPLRDTISALHQLKRSGLISQLPGFFCVYQLMF"
     gene            58932..59579
                     /locus_tag="ACHJ7J_000187"
     CDS             58932..59579
                     /locus_tag="ACHJ7J_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4998473.1"
                     /translation="MKTKWYDLAKQLMRAQGMSQDSLADLMGITKGGLSHWLNGRREP
                     NLEDIARIMRALGRRQFTVTHDGMVIDDSVSNTLPGAPPRDLGSYPVVDWKDVVNNME
                     DARRSTLPHVTTSVICSDDSYWLVAKGESMNAPQGLSIPAGTMILVDPHAPAIDGKLV
                     IAQLEEGQIPTFKQLIIDGGRKLLRSLNPLYPPIPMNPESKIIGVVVDAKIVNLP"
     gene            60086..60376
                     /locus_tag="ACHJ7J_000188"
     CDS             60086..60376
                     /locus_tag="ACHJ7J_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998474.1"
                     /translation="MTVTIMYGKCDSKLNSRERRRIKRENERKSSPAINKTDNVDKAI
                     RFASEERCKPNGIKERRKGAVKWYTENESGNYYHATQPRQLGEKPLDKVRYH"
     gene            60526..61167
                     /locus_tag="ACHJ7J_000189"
     CDS             60526..61167
                     /locus_tag="ACHJ7J_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO4998475.1"
                     /translation="MKTELHKKLWTIQQTLNAPKGQYNKFGGYSYRSAEDILEAVKPL
                     LQNVTLMVSDEIVLIGDRYYVKATATLSDGEDSISATAFAREEKEQKGMTAGQLTGAT
                     SSYARKYALNGLFCIDDAKDLDTDAYAKQTGQQPRQQKNQPKQQPQQQKAPPNPDEVL
                     ARFCDAAAKAQDEKTLREIFGKCWKLLPENSEQRQKAQDVYGIRKAELNGEMG"
     gene            61167..61583
                     /locus_tag="ACHJ7J_000190"
     CDS             61167..61583
                     /locus_tag="ACHJ7J_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4998476.1"
                     /translation="MSLNLITLGGNIGNDMEVRYTQNGKAIGSFPLAVTNGYGDNKRT
                     MWITCLVFGERAEKLAPHIRKGGKIVVSGRLDVRQYDRNDGTKGTAVEVAVNEFEFMN
                     VNQQGQQQGKQQKAPPPQNNNVNNPPPMDFDDDIPF"
     gene            61599..61877
                     /locus_tag="ACHJ7J_000191"
     CDS             61599..61877
                     /locus_tag="ACHJ7J_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998477.1"
                     /translation="MVDYLKTPPPPRTKEQVLKEARDHIDHGLFLCGTAAERMAKRFS
                     DLYAKQIWFDNWQASFYPLQRKPDMHWPEYVDPRMRKYRGRMGQVIND"
     gene            61892..62116
                     /locus_tag="ACHJ7J_000192"
     CDS             61892..62116
                     /locus_tag="ACHJ7J_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998478.1"
                     /translation="MIEEKEVDKEMPDTADDYVTFDGGLYEFDTSAGWHDKWPLPTRK
                     ELNDRKSFGEDAERLANNKWLNKFIAEGDK"
     gene            62235..62627
                     /locus_tag="ACHJ7J_000193"
     CDS             62235..62627
                     /locus_tag="ACHJ7J_000193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998479.1"
                     /translation="MKVGKDIVTVAFCIICFMFVVVFCGGFAALVSLIFRGAVVMKTW
                     DAKAGDLVVLPEYRDDPGLVVLNRSDCNMARPILVRYLDGVIIVPVYFARVELMARNV
                     RVKPFRAYVEDHWRKLFAGLNGMYGVWL"
     gene            62618..62809
                     /locus_tag="ACHJ7J_000194"
     CDS             62618..62809
                     /locus_tag="ACHJ7J_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998480.1"
                     /translation="MAIKEVKVKDLKGGERLKAWWGREFTAAAFMIMQGGEVVIYDED
                     LKEVGRWHLEQYIEVLDEN"
     gene            62799..63131
                     /locus_tag="ACHJ7J_000195"
     CDS             62799..63131
                     /locus_tag="ACHJ7J_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998481.1"
                     /translation="MKIKFHKAYDAETNKLSLFIEFERRIVVVPYSRRFNDAAGRRQF
                     ALDMLLRGCGLMRPLSDLKRMMPGSFGQIDEIEVSHEELKRARDLFLSSEGNPFNPET
                     EMKWHHPL"
     gene            63145..63765
                     /locus_tag="ACHJ7J_000196"
     CDS             63145..63765
                     /locus_tag="ACHJ7J_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998482.1"
                     /translation="MKDIKTAYSLGSDGMLITRYAEDASFHEVETKDYHEVLKDMEAG
                     AYDADLNLALQIVDIMMDASIRDYVSLNAEEKTAVARYVFCLTFVKRMEEEYGRVPVP
                     EEIDPLAFGSAVIFPLDENHTGSVSLHSMRGVMKEIFEAKALQKCIDEGHKEEDVKAI
                     MPLFYGEMVGNNMRANDLGVQAAIAVLNDVRKNVQSMPEPEKRVLH"
     gene            63864..64082
                     /locus_tag="ACHJ7J_000197"
     CDS             63864..64082
                     /locus_tag="ACHJ7J_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998483.1"
                     /translation="MEREYMLYCVDGGVGSDAYVAGKSYPADEVLSNMHFRENENQVS
                     VWKRCVDGIEVVSLERYLGKFDYALIEA"
     gene            64182..64376
                     /locus_tag="ACHJ7J_000198"
     CDS             64182..64376
                     /locus_tag="ACHJ7J_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998484.1"
                     /translation="MKKVGGYTLPDNAKLAGIGFSGCYYYNIAGDVYHVYFVDGVQHF
                     RRLPDVELIKLIREVSLHAV"
     gene            64366..64569
                     /locus_tag="ACHJ7J_000199"
     CDS             64366..64569
                     /locus_tag="ACHJ7J_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998485.1"
                     /translation="MRFKHYREWKIPEAATKAAPGNFSGVYFYMDGKWYFGSRPDHYY
                     QEICKPHVWDIKERVKDGVIEDV"
     gene            64570..64806
                     /locus_tag="ACHJ7J_000200"
     CDS             64570..64806
                     /locus_tag="ACHJ7J_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFO4998486.1"
                     /translation="MIREVFRFLIAWLLAALFIFLACAVVIAVLIVGAMFITWSLPDV
                     RSWTELLFGIRVLFAFSVFVGFCWVASPEWDDPW"
     gene            64811..65044
                     /locus_tag="ACHJ7J_000201"
     CDS             64811..65044
                     /locus_tag="ACHJ7J_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998487.1"
                     /translation="MALFNLSEPQFNAVKTAARAALSACKAEVEKNGYSDKATRLILD
                     KHYRKVAPLISIERFVWLVGYLNNRWGAVQDYF"
     gene            65054..65593
                     /locus_tag="ACHJ7J_000202"
     CDS             65054..65593
                     /locus_tag="ACHJ7J_000202"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO4998488.1"
                     /translation="MKNDYGGSHTPKEIKDLWQTPKPVFRGMDREFEFVADVAANKEN
                     ALVPRYITEEMDTLHYQWGAVAMPGEYVWMNPPYSNPGPFVDKAALEHKRNHIGCVML
                     LPADISVGWFMNGVETANECRLITRGRLAFINAATGKPVSGNNKGSLFLIWHPRCRHE
                     CIFTQITRKELYARGADND"
     gene            65586..66083
                     /locus_tag="ACHJ7J_000203"
     CDS             65586..66083
                     /locus_tag="ACHJ7J_000203"
                     /inference="COORDINATES: protein motif:HMM:NF040395.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6378 domain-containing protein"
                     /protein_id="HFO4998489.1"
                     /translation="MTSAADLLRLAAETIEQRGKQNGYDRKEEKSAPKIATIYNAKKG
                     ANLTPLDVWDLLICLKEARLEAILVNESDPTDTLVDLISYSALKAEQILNDQEEEKKK
                     KQFNAANIADGIINDGAITSCKGLPPSMLNPQCGCIPTSVGVKFNRDNAIRDKRFDPN
                     EKGDN"
     gene            66064..66567
                     /locus_tag="ACHJ7J_000204"
     CDS             66064..66567
                     /locus_tag="ACHJ7J_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998490.1"
                     /translation="MKKVTIDRLLACVYVAVFVIINLIVNHCGPWVIPITTVAAVCVN
                     MMIRDFLLYDGGLKWSATTCAAAGAITVMINYDAGMVAIASFVAVVSGALISGGVYRV
                     LPGDFDSKRWPANIASAIGDALIFPTLSFMAFMPEISAMQFISKMAAVTVITIIMRRY
                     FTFEGRK"
     gene            66564..67346
                     /locus_tag="ACHJ7J_000205"
     CDS             66564..67346
                     /locus_tag="ACHJ7J_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO4998491.1"
                     /translation="MSRAKHWLNNWLRSWVVWSLYDGSGYAVKDWAEAGYKCYCFNYD
                     GANHGDYEGVKIIHPNIEYVNVWIDSHFLVMFSPELSVYPDPDIIFGFPPCDDLAVSG
                     ARWFSDKRKKDPHFQVKAAYNAKIVEKLANMYNVLWMVENPVGALSTLWRKPDFIFNP
                     CAYGGYLPEDDKHPAFQDVYPARDAYTKKTCIWCGNGFKQPLFRPVDLNSGDNPGWAK
                     TGGRTKRTKMIRSLTPRGFARAVFLANDRAITRTTLNRVLPD"
     gene            67697..68302
                     /locus_tag="ACHJ7J_000206"
     CDS             67697..68302
                     /locus_tag="ACHJ7J_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4998492.1"
                     /translation="MQNPKKPIRRRCKCCGVFFEPKYHNQTWCSDECLEELKFDQLCR
                     DREKAMKAMERKKRRDSQREERNRKRKQLNPRSYWIKQAQNVFNAYIRERDALLPCIS
                     CGTYYGEQCGWDAGHYRTVAAAGHLRFNDDNCHKQCRHCNQTLDGNIGGYRPALIRKI
                     GLARVVALENNNETHKWTIEECKEIIKVYQAKLDALRRKAA"
     gene            68299..68727
                     /locus_tag="ACHJ7J_000207"
     CDS             68299..68727
                     /locus_tag="ACHJ7J_000207"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4998493.1"
                     /translation="MNEYTFSLPYPPSNNRYYRHSRGFHYISKVGKEYREQVKDIIEL
                     LNLNINLSCRLAITIHAAPPDNRIRDLDNIPKCLFDSLTYAGFWTDDGQIDSIKIVRC
                     RKVKGGRLFIKIRERGDLLPDIDEYATNMWGGNGDNKHCC"
     gene            68934..69116
                     /locus_tag="ACHJ7J_000208"
     CDS             68934..69116
                     /locus_tag="ACHJ7J_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998494.1"
                     /translation="MKNEIKDLQIDIQRDEGDLETVRQIQAFHMRELLALKEFERKLV
                     QAISDRKRLVARYGGK"
     gene            69116..69838
                     /locus_tag="ACHJ7J_000209"
     CDS             69116..69838
                     /locus_tag="ACHJ7J_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4998495.1"
                     /translation="MNLESILKFHFPKSPRLSDESRGTSPDALNTTDALTAAGMAQSR
                     VELGYSAFLGKMELSQAEKHRAVVLLTERLRAMAKDYKYVMELNEAQRNDLIILVAVF
                     AFRDYCQSAATEKTCMECGGYKFTFDEYGEKHLCKKCKGHGKVLNHCKQCKGRGEVPD
                     KAASEAAEMPVFKTCQHCGGRGYSRFPVDLVRQAVNQLVFPVSRSTWWKKYRVFYEDA
                     IAELFKEEARADNEIKRVTRGE"
     gene            69835..70038
                     /locus_tag="ACHJ7J_000210"
     CDS             69835..70038
                     /locus_tag="ACHJ7J_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998496.1"
                     /translation="MMDKLEINDQFAVILFEDKTGGAWCKKVTGAEARLILGMASALN
                     DGELPAVPIEPVHIYGRSNDNEA"
     gene            70028..70297
                     /locus_tag="ACHJ7J_000211"
     CDS             70028..70297
                     /locus_tag="ACHJ7J_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998497.1"
                     /translation="MKPKLKEHLFAKLVNDLTLSARLCGDTQQLRAWIARDLRKYIEP
                     GGPGDEMTMDKAIKRRSADWSVSNTGLTGYIEGYNDCLQDHSDGK"
     gene            70287..70586
                     /locus_tag="ACHJ7J_000212"
     CDS             70287..70586
                     /locus_tag="ACHJ7J_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFO4998498.1"
                     /translation="MASKDLHLNLKGEYFHAILTGKKVEEYRLYNDYWRKRLEGREYE
                     RLIIKWGYPANHEKHRIINLPYFGYEVKTITHQLFGPDPVNVFAIKCNRNWMLRG"
     gene            70589..70801
                     /locus_tag="ACHJ7J_000213"
     CDS             70589..70801
                     /locus_tag="ACHJ7J_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998499.1"
                     /translation="MKSNRKRLVRAYDKALKAFDDLRRNKRQRRKWARMLVSEWHDED
                     FFLEARHMTQDVADALADENVYYMMW"
     gene            70806..71171
                     /locus_tag="ACHJ7J_000214"
     CDS             70806..71171
                     /locus_tag="ACHJ7J_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998500.1"
                     /translation="MQIIIDYLCHAANTVFGMYQQPFLKEWDEMLNDIIDKGLIVEVG
                     ELTIKFNYEGKEYDIWVGNRWYAYGHIYSIGGKYIKRRQEFRPRFRTMRRLRDLHMNI
                     FENQEACELFKIYGDKSWN"
     gene            71162..71392
                     /locus_tag="ACHJ7J_000215"
     CDS             71162..71392
                     /locus_tag="ACHJ7J_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998501.1"
                     /translation="MELKIWCAIDVVDNELSMFATDGKRVVIATWTRNHDDIAFRRKA
                     AEWLFSDGGYTMNIAQLARMKDEILVDSYTTA"
     gene            71358..71633
                     /locus_tag="ACHJ7J_000216"
     CDS             71358..71633
                     /locus_tag="ACHJ7J_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998502.1"
                     /translation="MKYWWTATQLHNGVNMRIYEHKRDKTRFFVRAGVAYQYHECGYI
                     EALAYDLDFEQEKAWFDFKIYRKRKPTRDERHAIRDFLISIERWESD"
     gene            71633..71860
                     /locus_tag="ACHJ7J_000217"
     CDS             71633..71860
                     /locus_tag="ACHJ7J_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998503.1"
                     /translation="MKVKFLHDHGYPSLKQVVGKVVNVVHSDNVTCMINGADLIAAGA
                     DDHYINPAWSYTFSLGDFVGDKGRGLQVVEG"
     gene            71961..72113
                     /locus_tag="ACHJ7J_000218"
     CDS             71961..72113
                     /locus_tag="ACHJ7J_000218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998504.1"
                     /translation="MKEWLFKRKDLLDDLVFAGIFRKRPANLEEEMLIEVIKNENQGK
                     LFPGER"
     gene            72076..72327
                     /locus_tag="ACHJ7J_000219"
     CDS             72076..72327
                     /locus_tag="ACHJ7J_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998505.1"
                     /translation="MKIRVSYFRAKDKATGKQMAILVNEAQYMFVLQPWCVVDLNDNC
                     RRHAARSAVGMKGWQPRDMENYCDWKLVAKYTVDYKGVF"
     gene            72346..72552
                     /locus_tag="ACHJ7J_000220"
     CDS             72346..72552
                     /locus_tag="ACHJ7J_000220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998506.1"
                     /translation="MQVKCVESNSDIFVVDKIYHVYQVLGVDCDERDQVLIDEDGDPW
                     KFLYRYASGGDVIGIRLRAKFELC"
     gene            72562..72861
                     /locus_tag="ACHJ7J_000221"
     CDS             72562..72861
                     /locus_tag="ACHJ7J_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998507.1"
                     /translation="MKSDKFIACCFVEVPKGDVFCNYEFKDSLGRSHVVVGKRGDWLY
                     ITDGDRIFTKNQLFRIKAENNEIPVGNMGIMNYHACLCLKHDAVNKARSLPGAEF"
     gene            72885..73208
                     /locus_tag="ACHJ7J_000222"
     CDS             72885..73208
                     /locus_tag="ACHJ7J_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998508.1"
                     /translation="MSKFISVKVFRGTLPNEERLGQFAGQPGACFRVAAEDDAHVKCF
                     HVSEPPFNADDLENMDRIKGLVFAYLMFQGIADMEVELLSAELVAEYKMTEAEEGGIK
                     IERIK"
     gene            73192..73446
                     /locus_tag="ACHJ7J_000223"
     CDS             73192..73446
                     /locus_tag="ACHJ7J_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998509.1"
                     /translation="MSASNKLYKVRCKGEYPGFTIGCEYLGHIGYGHFGELGMNTMDD
                     DGDSMTLDLDSDDFEYIPPVTCRVVDEFLAEHEDDEDDYD"
     gene            73433..73633
                     /locus_tag="ACHJ7J_000224"
     CDS             73433..73633
                     /locus_tag="ACHJ7J_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998510.1"
                     /translation="MIMIECLIAVTVLLYIAGAILMSCFIKFSGAQPGGLEIPLWPVV
                     VFLAFFDATAEQIRRAIKERKK"
     gene            73630..73812
                     /locus_tag="ACHJ7J_000225"
     CDS             73630..73812
                     /locus_tag="ACHJ7J_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998511.1"
                     /translation="MIEDGIYGVNLMENVFYMVEGDTVRISIDGGEYVETFFKTTKED
                     IKLGVDKGVLFKVGEL"
     gene            73809..73985
                     /locus_tag="ACHJ7J_000226"
     CDS             73809..73985
                     /locus_tag="ACHJ7J_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998512.1"
                     /translation="MNEIEDGIYLHKVFNIVYLLKGDKVMIRPDDDPSWELSDMDRWH
                     MQRLLDNGLIYRKQ"
     gene            74093..74293
                     /locus_tag="ACHJ7J_000227"
     CDS             74093..74293
                     /locus_tag="ACHJ7J_000227"
                     /inference="COORDINATES: protein motif:HMM:NF027408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO4998513.1"
                     /translation="MFDRIRAACAYVTGTVTAFFGAITINDVAVFVGILSTIGTFAVN
                     YYFKSQENKRAQEEHDARMGNK"
     gene            74295..74786
                     /locus_tag="ACHJ7J_000228"
     CDS             74295..74786
                     /locus_tag="ACHJ7J_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4998514.1"
                     /translation="MISQSLKNKIVAAAAGGAISIAAVMIKPLEGVDYDPYRDVVGVW
                     TVCYGHAGKDIVLGKTYTQSECDALLNKDLHKTAKAIDPYIKVEVSDFTRAALYSFAY
                     NVGATNFKTSTLLKLLNDGKKSEACAQLKRWVYAGGKKWQGLVNRREVEYAVCEWGET
                     WTR"
     gene            74774..75244
                     /locus_tag="ACHJ7J_000229"
     CDS             74774..75244
                     /locus_tag="ACHJ7J_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4998515.1"
                     /translation="MDKVKTLIIAAVVCIIASLTAVTCHYHSEATRLQEELTTTQGAL
                     KTASNTIQQMKERNAELSKLDKRYHDEIKAIRSDIADLRTGIDNGTIRLRVNAIPVRV
                     SDSTGSASAINGASCRLTPDAESAYLSLREQLKEKDAKITALQGYIKTQCLRKE"
     gene            75574..76116
                     /locus_tag="ACHJ7J_000230"
     CDS             75574..76116
                     /locus_tag="ACHJ7J_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998516.1"
                     /translation="MAKAKGIKLPQFKVPLFEHTTVFFCPTRDMFYEFCEKAGIPIEP
                     DFDLAGGLTLTCTGEKGGNFYVIAVFDNELGTLVHECAHTTFHVLSDVGVVATTDPTH
                     PANETYAYMVGRIFDAFFPILAESNEAQVAAMQAAEVVEQALEQAEQPKEEEKPAKKG
                     KRKPKAKEALVPRVMSFKRG"
     gene            77122..77607
                     /locus_tag="ACHJ7J_000231"
     CDS             77122..77607
                     /locus_tag="ACHJ7J_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4998517.1"
                     /translation="MVRTKKAKADDKKPAAKKTGRPHGYTEEKALEICELVSDGESIN
                     KISKMPGMPARSTILKWFRDVPEFSDMYMRAKEIGFEVLADEIIDLADAPENIKKEEL
                     NRHHLMIETRKWLLAKLQPRKYGERVTQEIVGNRDEAPVQVEVTKEEIARIVQEVEDE
                     V"
     gene            77610..79088
                     /locus_tag="ACHJ7J_000232"
     CDS             77610..79088
                     /locus_tag="ACHJ7J_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004136203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO4998518.1"
                     /translation="MLTIKERVIQSKCEKDGLFFNRYFYKQANGTKMLISGHHLAIRD
                     ALQRVINGEITRLIINIPPGYGKTMIATINMMARSLAINPRTRFLHVSYSNNLALLNS
                     STVRNMICTPEYQAMWPMKIRNDANSKSMWWTEYGGGVYATSSLGQVTGFRAGYMESG
                     FNGALIIDDPLKPADAYSDVVRKQVNTNYNDTLASRLAVQTTPVIVIMQRIHYDDLSG
                     YLLRGGSGEKWYHLNLPVKIDNSMDYWDLYPENEFAIPIAHNLPDGWLWPKKHNDIHE
                     AGLKAHRRSFEAQYMQRPRKFDEEGALWTEAMITAAHRMQITQDKVRTVIAIDPATTS
                     SDESDETGIVACSAYGGGKNAQYSVDGDYSGRMSPNDWAQASMNAYNIHEADAIVIET
                     NQGGEMAEATLRNAGFKGRIVKVHASKGKFARAEPISALYAQGRVAHTGSLYTLENQM
                     MEYVPATAKKSPDRMDAMVWGITELSQPQAMGLMLPKRLRGF"
     gene            79167..80537
                     /locus_tag="ACHJ7J_000233"
     CDS             79167..80537
                     /locus_tag="ACHJ7J_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4998519.1"
                     /translation="MPSNLDLAVNAALSQRQAAYARYAAANPFTMGIDAKRDSAWSEY
                     GFKEEITYEDLYKLYRRGGIAHGAVEKIVTTCWRTMPTLIEGTEDEKAEKETPWEREI
                     KKQFDNRFWRVIAECDRRRLIGRYAGLLIHVRDNQPWEKPVTKGVGIAKFTPVWAGAL
                     TPKEFEEDPDSENYGLPTWWEYKERINSKTIARRIHPDRIFIFGDYSDDAIAFLEPSY
                     NAFVSLEKVEGGSGESFLKNAARQLAISFDKEIDFRSLAATYGCDVTELREKFNEAAA
                     EMNKGNDVMMALQGATVSPLVTAVSDPSATYDVNLQTAAAGIDIPTRILVGNQQGERA
                     STEDLRYFNSRCMTRREEIGGELEDLFRKMADLRLISMPIDVSVIWDDLNAMTKAELL
                     EAAHKMAQINQACLATGEEIFSGDEIREAAGYDGPASVVEMEEENDDEGEEDNQANTS
                     SRDNAI"
     gene            80527..81423
                     /locus_tag="ACHJ7J_000234"
     CDS             80527..81423
                     /locus_tag="ACHJ7J_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO4998520.1"
                     /translation="MPSNTEDPTMTGKLRSGAIKRFKACLKKVADPYIAILDRMQYSL
                     AVNKKYTFQIYIDELHDLLEDASDMIDEIFELTDPENFWFWQEYVKVAYQRGTSQEYA
                     NLANQSVTYSRAYPEVSAVLTSQTYRTRLALVRTRVFEEMRGLTAQIKKDMARRLTEG
                     MARGLNPLEIARTLQQETQLPLYRCKRIARTEICTALRTARMDEAEAATEELNLRTMQ
                     MHISALSPTTRLSHAQRHGKTYTIEEQREWWSRSPNSINCKCSTITVLVDESGNILNK
                     RILERAQENYKVAHAKYGEDWE"
     gene            81429..82700
                     /locus_tag="ACHJ7J_000235"
     CDS             81429..82700
                     /locus_tag="ACHJ7J_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998521.1"
                     /translation="MSKELIQVNTKLTANTIRRETYNGREHIVVPSYTLPFNIVMNRE
                     YYPEAEIIANYQSLEGTLAPLGHPTVDGEFVSAFSPEGLNIGFCGAWNRNVELRGNRV
                     YVEKWVDVETASHSEQGRELLSRLEALEKGESKDPIWSSVAVYRQRMPATEEMKAQGA
                     DSVVKIMSIDHDAILLHEPPAASPEQGVGLMVNTDQAKPLMAVAMKENSYRTLERQLE
                     EAARKLFPDADYVYVVDFTDKEVTIATNTESAQVCTYEKQADKIILNNGELATNEEGK
                     SWFVQFAEHLSNLFSLNEKIKANKSEGEPMPLTKEERAELVKEINEGLAANIANAVAE
                     ALKPVQASVEELQTNQKAIKEEIAANANKEEAEKRAAVAKVHGEIVANALSGEALEAM
                     FKSLGKAAPMAANSAAGGNKPATPDINNYFA"
     gene            82711..83112
                     /locus_tag="ACHJ7J_000236"
     CDS             82711..83112
                     /locus_tag="ACHJ7J_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998522.1"
                     /translation="MARFRRVNIDGKSVTETAVSAAALKPGTPVKMAAGKFAAATDTT
                     GRIYVVNPAYHEGLGIEDAIPVGHSVVADYAEEGREFAILLPEGTYTKDAAITIGEGG
                     FKLVASDEPVFAFCQEAVTLEAADFVRVRVA"
     gene            83132..84184
                     /locus_tag="ACHJ7J_000237"
     CDS             83132..84184
                     /locus_tag="ACHJ7J_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4998523.1"
                     /translation="MLFNKHNLATNSRIRAQWDHLWAQRNMFNDRDGALIAANMANMT
                     ADILACNAVGGFDQEFWKAIDNQIIEMSTEETGIEIVNDLMAVQTVLPIGKTEKMYSV
                     SGDINDEVVVSIDGQAPHGFDHTEYGSDGDPIPMFAAGYGVNWRHSEGLKTVGIDLAL
                     DSQRLKLQKFNKARVDYYLNGNAKINVKGKPGQGIKNHRNTQQLVMTSEDLTTDGFDD
                     LIKFFTTGAFGVMARNNRVDQYDIMWVSPEIMANMAAPHIANGTVVGTVLNIVKPFIP
                     VKEIRQTFALKGNEFIAYQRRRNVITPLVGMTTGVVPLPRFMPTDNYNFKIMSAEGLQ
                     ITCDMLGRSGVVYGHK"
     gene            84250..84636
                     /locus_tag="ACHJ7J_000238"
     CDS             84250..84636
                     /locus_tag="ACHJ7J_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998524.1"
                     /translation="MVTAQQAREYLESQGVELPEVVLSLLVEQANSVNECLDANYPAS
                     TATLIQLYLIGLLGLTQANKYVSSHTGPNGASQSYRYVDFNKKWKAAYSLLYSLDKHH
                     CTAELIPPDPENTAHAGLWIGKSGRM"
     gene            84639..84824
                     /locus_tag="ACHJ7J_000239"
     CDS             84639..84824
                     /locus_tag="ACHJ7J_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998525.1"
                     /translation="MWNDLTLPKPLLPKLFTRVWVKTDTGRQVAAYLNDAGEWVILCP
                     RVAKTHPKIVKWSYGYE"
     gene            84817..85185
                     /locus_tag="ACHJ7J_000240"
     CDS             84817..85185
                     /locus_tag="ACHJ7J_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998526.1"
                     /translation="MSKIARFSYKALATIYPVKRDDWTNSDVYGTPYLIDCSWERTDG
                     TATDANGNEVSNTITVYTELLHKMQPVQRPEKGWMLATGDTTAIADPLAAGANFITGI
                     VEWDMSMFNDTPDYKIVTGG"
     gene            85188..85661
                     /locus_tag="ACHJ7J_000241"
     CDS             85188..85661
                     /locus_tag="ACHJ7J_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998527.1"
                     /translation="MPLKGVRRVRMKMTEEIRNIADKRTYEVLWMVGNVASGLASGMT
                     PVDTGFLINSMYQTVEKNGNGLCLSVGYTARYAEWVHDMPGTLMGQPREHFGKTNNLS
                     DFGPKQVVEFGGGTGKGYYWDPNAEPEFLRKAFEEPDNFNEIWNTIKLGYWTKQS"
     gene            85658..86044
                     /locus_tag="ACHJ7J_000242"
     CDS             85658..86044
                     /locus_tag="ACHJ7J_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998528.1"
                     /translation="MKRSEVYDEIRDWIKSHGYDEGYILQARFWNERSNSNNDRYIVI
                     QQNGGAAGEEAVTRDYFRILVISARNDANISEVEDLADAIRQSMITEYKTDKITHMKP
                     VGAIPAMQTREGRFIFTVAFQTIISR"
     gene            86057..86533
                     /locus_tag="ACHJ7J_000243"
     CDS             86057..86533
                     /locus_tag="ACHJ7J_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4998529.1"
                     /translation="MSQTCEKGSFLGRDVAVFYAIACPNAKPETDAYKALGMMRGKSL
                     SVEWETADATADKSADYTKESLVTYKSVAFSGDGISRTEEIHNQKALKRHVINPGETT
                     GAQPYVWLKLVSPLDVTEGPFLCTSFKEEDPHDDVSTWSIECESAGKVTVGDVPSV"
     gene            86588..87265
                     /locus_tag="ACHJ7J_000244"
     CDS             86588..87265
                     /locus_tag="ACHJ7J_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6246 family protein"
                     /protein_id="HFO4998530.1"
                     /translation="MIHVRTGQFAVVVDGRRYVFNPCFAAMAKIGSDRELVEYFATVH
                     GGKYPQRLPTDTDLRNRILARCYGELVQTSIHVLKCCSEDEIGPLLGECRFTPSGKLI
                     LKPGLMPTGDIITLAQHCMYHGLIGDGPEEDASATQEGEYKPTFDVLEFVYSAVAHLG
                     LSESEAWDMTMTGYRAAVRAKTPPDERNERSKPNVHINKRDYDEQMEAAKRVLELMKN
                     SEQKKAR"
     gene            complement(87308..87724)
                     /locus_tag="ACHJ7J_000245"
     CDS             complement(87308..87724)
                     /locus_tag="ACHJ7J_000245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998531.1"
                     /translation="MKRLIKAAMMSVMVGLAFNANANEEINIDCDEVANNVAEYHKIF
                     KKEPAAIKVFYKAIDKHFEGKPVYARWFNYGLVKEAAVGVVFEGNNEIRNRIANECNA
                     DKAAFAERIFNEGGAGVKNHAIVDLGNGRIEIVRIR"
     gene            87977..90325
                     /locus_tag="ACHJ7J_000246"
     CDS             87977..90325
                     /locus_tag="ACHJ7J_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4998532.1"
                     /translation="MATSLGTIYYEVDAKTGQLLVAQRQADQAFDSIERGAKRADRQV
                     NTLKTSIKALTRVIHLLLAAEAVRQFIDMAEQAKMLRVKIKLLTGEAEATERVFDRLK
                     EISKETGQSLKDTGDLWQGLAISLKNTSATEGQVLNLVSTLQKLGNLGGVSAEQLSNS
                     MRQFRQAIDAGVLQAEEFNSIRDNTPTIIQEMARQMGLSMGQFRAEMLDGKITAERML
                     NAIQAATQETNEKFAQLPRTSGMAFNELKVEVMGLVEQLDDLFGISDGVVTAIDLITG
                     GVEGLGKGAKFAATCFNTLKTAGSEFIDMFDDVAVKAGEVAEEIIRMVTPIKALMDGY
                     KWMKEIIDKRKEELNSNNEKKFGPTIGKFMTFKNDIEKATAAYDEFMQKQGEADDGKI
                     TGFDQPVGKPKKGKKGKKDKKSEADRLGDEGIKVSDQYNKDAAAMRKALENGKAIDAA
                     FAQGKITLLEYKAAQKGIGKELKDELAQIPVEELQDKWAHIVSPMDQLKGEIDPIKQA
                     QNEWAVRKQMLLDLGATEAQQRQAQLEYEQQIRDLKWEQWQAQSDTNGLIGACVSGLK
                     GGMGNALTGLLNGTQSLSEVFANLGSSILNTVGNKLSEIAANWIADQLMMETQSKATQ
                     ASTTAGAVAAQGEIAAAAAPAAAATAASTGGSWAAAGAAALSAIMSLATSIFGGGRYN
                     GGSVNGGSLYRVGEHGIPELFQTSNGRQYMIPGENGRVIPGRDLFSGGGINMPVNITV
                     QTTNGFSDEDSRRLEQTMERVAMKMMARESQRPGGMLQPRRK"
     gene            complement(90357..90536)
                     /locus_tag="ACHJ7J_000247"
     CDS             complement(90357..90536)
                     /locus_tag="ACHJ7J_000247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998533.1"
                     /translation="MIIHETEECRSINLSIEDDGRLWIVSDCEMIVIDKKQAEELIRA
                     LQLYVNGEFKNDEIE"
     gene            complement(90864..91118)
                     /locus_tag="ACHJ7J_000248"
     CDS             complement(90864..91118)
                     /locus_tag="ACHJ7J_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998534.1"
                     /translation="MKLVAIDKNLKAQKNAPGHVIKKAKELLNAFLNKDVAPKKLKDG
                     YGYAFRANLDWRVYSQDLKTWAIVEHHKFNELSGVRGCHK"
     gene            complement(91137..91292)
                     /locus_tag="ACHJ7J_000249"
     CDS             complement(91137..91292)
                     /locus_tag="ACHJ7J_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998535.1"
                     /translation="MERYVVVMLNNAFEQVGVAIVKGFDDAFKYGQFMMNAKEDEYRD
                     FFLKVVN"
     gene            91466..91819
                     /locus_tag="ACHJ7J_000250"
     CDS             91466..91819
                     /locus_tag="ACHJ7J_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4998536.1"
                     /translation="MPEVFRWTPQRSYSVTREPNVSVVKLGDGYEQRQEKGINTLLDS
                     YTLVFKGSSAGCGDSGNVAIQAEAFLRARGAVEAFYWSPSMDGVQRLFVCRSWSMTKD
                     GPVYTLNATFEQVVR"
     gene            complement(92049..92186)
                     /locus_tag="ACHJ7J_000251"
     CDS             complement(92049..92186)
                     /locus_tag="ACHJ7J_000251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998537.1"
                     /translation="MKIEVLALIVMGLIVFGVHAADWDWAAIANNMAHAHPMSLMYIN
                     Y"
     gene            complement(92279..92500)
                     /locus_tag="ACHJ7J_000252"
     CDS             complement(92279..92500)
                     /locus_tag="ACHJ7J_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4998538.1"
                     /translation="MYTLKQAARIIGYHEHEFIDLLIERGILYQVCLTLYPKAKYLQE
                     KLFIIMTDENQVNHSFVTDKGVNYLRDNL"
     gene            complement(92505..92990)
                     /locus_tag="ACHJ7J_000253"
     CDS             complement(92505..92990)
                     /locus_tag="ACHJ7J_000253"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO4998539.1"
                     /translation="MKWHDIFTYNEGKLFWKIPTSGRITNGKEAGSLHNGYIVICVQN
                     KKHKAHNIIWEMHNGPIPEGYVIDHINHIRSDNRIENLRMITKQENMKNMPLSRRNKS
                     GCVGVWWNETNKKWIVRVMIGGVYRHIGCFNNYLDACNASKEAHEIGGYYKEHGAAQS
                     R"
     gene            complement(92996..93343)
                     /locus_tag="ACHJ7J_000254"
     CDS             complement(92996..93343)
                     /locus_tag="ACHJ7J_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998540.1"
                     /translation="MQKYRLDVWTDKCEWERMGLFDTYAAARDEGRELLRCWSNARDF
                     DITPVQVPDKVEEMNTKDINFNVNVDFADSMDQSCPGEIHAKGFPADDPEAKREYEKA
                     AKDFCIDNLSCLD"
     gene            complement(93356..93511)
                     /locus_tag="ACHJ7J_000255"
     CDS             complement(93356..93511)
                     /locus_tag="ACHJ7J_000255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFO4998541.1"
                     /translation="MADKVWRLTVFLADGREMTVALYKDEGEALTDALLLAEDDRVLG
                     YRIEPVK"
     gene            93640..94344
                     /locus_tag="ACHJ7J_000256"
     CDS             93640..94344
                     /locus_tag="ACHJ7J_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4998542.1"
                     /translation="MRSIPTEMIIDSVDAGVGAVIDLFELDLTPLGGEVIRFHSGANG
                     YYGPVIWKGVAYNSYPIEATGFEMKNEGVYSRPQMVVANIGGLITGMNNDFNDLRGMK
                     VTRRQVEVKYLDAINFPNGNPDADPSIEAVSFYVVEAMSEETADQVQYELSTPIDADK
                     AVIPGRTILADVCQWQYRGDGCGYSGGPVATDKDESTSDPKLDRCSHRLSGCRLRFPR
                     PNPLPISCFPGSSKVG"
     gene            94347..95078
                     /locus_tag="ACHJ7J_000257"
     CDS             94347..95078
                     /locus_tag="ACHJ7J_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4998543.1"
                     /translation="MVMEDKMLRYAAAHPFEEVCGLVIDNDYFYPCANVSETPYNSFK
                     ISPDDYIKADELGVITAVFHSHTDNSLVLSARDRQQQVISGLPWFLCSGGRVRKFRPV
                     AHLLGRKFEHGKTDCYSLFRDAYHLCGVDLPDFERLDGWWLRGENLYLKNLPLNGFYQ
                     VDAQSIQPGDVIIRQPFKGADPCHAMIYLGDNTVLHHDNAGLLSRREQMRPAYVRQTN
                     SIWRSDKCSNLDLRAIFEDITARCV"
     gene            95021..95551
                     /locus_tag="ACHJ7J_000258"
     CDS             95021..95551
                     /locus_tag="ACHJ7J_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4998544.1"
                     /translation="MLKFRFTGNLRRYYGKVCLNVDTPAQGLRLLTAQNQEFKKAFLN
                     TPLRLRIAGKDYDEKTAPAAVNSKYPDWTTVIVAPVVEGGIAGIGVVGWILIGVSVVS
                     VAFSIFMSRNMKIKTSAESAQDNTISNNTYTSVENKVGQGRPVPILLGEMKIGSNVGS
                     LGIDTSNNRDALDVVS"
     gene            95565..98984
                     /locus_tag="ACHJ7J_000259"
     CDS             95565..98984
                     /locus_tag="ACHJ7J_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4998545.1"
                     /translation="MSSGGGKAKTPTLLNDNLYHKQFYRVLDILSEGPIYGPVNQNAP
                     LNNVMLNDTPITDANGGTSVPGVSVAWRNGTADQSPINGFNAIESTVIVNAKVTHDTP
                     IIRTVSDPNVNRVRLNLGVDSLVQSDDKGNQYNTSVVLMVDVKPSSSSTWSLVKDITI
                     GPGKQSGEYLEAHIIKAPDEKPFDIRVRRVTPDSTGDLLHNDTRWSSYSEIIDDNLSY
                     PHTAVAGAVIDHDQYTDTPSRTYHMRGLIVDVPDNYDPETRTYSGLWLGGFKKAYTNN
                     PAWLFRYLVKNERFGLARHAGYIDVDDGALYTLSQYCDQLVNDGYGGFEPRMTLNAYI
                     TEQMSARDLLDNIAGMFRGIALWDGQRLTVMIDAPQDPIATITNANVVDGAFTRSSIA
                     RAESYNAVIVSWTDPENGWEQSKEYVADDELIARDGYNETTLEAFGCTSRGQAYRAGK
                     WLIETAKREPSKFTFKMARDAIHFTPGDIIEILDNNRAGARLGGRIVANNGKVITVDK
                     VDSEYIAAGDTISLLDSDGKFKKHQITGVYGNNITLAAAPAWIRNGTVFAVSTESAKP
                     VLCRITSVAETENNSVYTIEAAQHDPHKQAVVDEGAIFEVNNDSLNHFRVPNIENLKV
                     LNVGSETVQCRATWETQTTTHRLTFEIRVYNADGRVVVSYETTNYRYEFYGLDAGSYT
                     LGIRGRNDSGMKGAESIVDLVIGAPAAPIGVNWVPGVFQATVYPISKTTLTSDTSYEF
                     YFAGETQITNPALITTNAQYTGRGYQWTFGGMKTDHVYYVYVRTRNAFGVSDFVEASG
                     KPTDNFDEITDFVTKDVMNSQQFKDLISDTSAIKDDVDNLKGATSDLINRVQNNEEDL
                     GVHKSRIDEMGVEISEQDSELAQARARLQNVSIALLNNSVAQTTSGRTLSAQLKQSRA
                     EITRIDEVVATEKEATAKALTTLEAKINDEIKGSVTNLEQTMATLEKTTAQQISEVTA
                     SINGQSVLIEENKTAIAGISGDMSASWSIKVAADNNGMKYATGMSFGLTGDGTNVQSQ
                     CIFLVDRFVLMTAANGTYTTPFYVANGAMYVREAFIKDASITNAKIGSYIQSNNYVSG
                     STGWKLDKGGTFENYGAVSGEGAMKMTNTTISVKDGNGVLRVQIGRITGSW"
     gene            99035..99346
                     /locus_tag="ACHJ7J_000260"
     CDS             99035..99346
                     /locus_tag="ACHJ7J_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998546.1"
                     /translation="MATFGVATWDANGNPNNYGIKPSVVVGYISLSSGQVSGTYTFTV
                     PAGCKLSYCMTLDYGTMSTTRRSVTVSGGTVSISSAGDTVGANIWPASQCEIVIFAEV
                     A"
     gene            99346..99939
                     /locus_tag="ACHJ7J_000261"
     CDS             99346..99939
                     /locus_tag="ACHJ7J_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998547.1"
                     /translation="MATFGAYLRLSNGNIFVTPEATPLCLYRRVTLTSSYQTVAVPSN
                     KPVMVFVKSHTASPVLAYRDTSSTVMIGAGNGYAYIFTIFTPSLPKWGMAIWNASGEL
                     VITNESRILTDLVTVGVAGSSGGIYIDQTLSGSYAICPQLLGAQVGQGAASIYAAAYY
                     NGVNTRIYGIKVGGVYAPTGFINHGNAITAIKTDAYD"
     gene            complement(99936..100175)
                     /locus_tag="ACHJ7J_000262"
     CDS             complement(99936..100175)
                     /locus_tag="ACHJ7J_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998548.1"
                     /translation="MKCLKNIAIAAIVACLFGCADMPKRECTAIYQTGGNDYAVAVFG
                     VTDVDGHKMIKAGYPFNFQWVSVDHFKKTDCPELN"
     gene            100335..102578
                     /locus_tag="ACHJ7J_000263"
     CDS             100335..102578
                     /locus_tag="ACHJ7J_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFO4998549.1"
                     /translation="MIYTTGTIAINGNTVTGTGTDFSAPLSLIRVGCTLIAVGNPVQI
                     FTITEIKSGTELSVTPAANPAIPAGTKFSILLSDSISVDGLAHDVAETLRYYQGKETE
                     IATAVEWWNEFGGDGQMDQLLADIRSETAQSTANAQKTESDKNAAAASKTEAANSATA
                     AKASQDAAKASETAASNSKTAASTSETNAAKSAADALNYRNQAQGIVGTSIGLGSSPR
                     DCPDISGDPSGYIGFMRIMEGATGFPSIASGDGNLTGFISQVDESPAYTGIFQGWNTR
                     SIYTYRWFSGLGPQWSQHARKDEVSRLTQGSGESALWAAGGKQKFVLLDGGDGNTLSW
                     GVWDNVASKWSPLSIAQGGTGAIDALTARNNLGVGVENSPQFRDMILSHTTDESSGWV
                     SGGVQKLVLLGSEGTKRAEGLAYAECNPDQPTQLTFAIYGVNEGYNYLSFNTMGQILA
                     ETAIFRSNYANPLVIKSSTPTICFNEEGRPDGSPFYNLIFDDGNWRIQKNGDGYNGEY
                     VIGYNYTLDEMELPNLLTKAIKLNSNDHEKSLLIRVEDSTKFSTLIKVNGNNTGRDSV
                     FELDFLNKETAEYDNYGFYCQILTDNSIQFQVNGAVNCTTLNQSSDRDLKDNIQVIGD
                     ATEAIRKMSGYTYTLKENGLPYAGVIAQEVMEALPEAVGSFTHYGKWLQGPTVDGNEL
                     REETRYLNVDYAAVTGLLVQVARETDNRVTELEEENASLRDNIAAMDERIAKLEALVS
                     QLTGSEK"
     gene            102971..103018
                     /gene="ydaN"
                     /locus_tag="ACHJ7J_000264"
     CDS             102971..103018
                     /gene="ydaN"
                     /locus_tag="ACHJ7J_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO4998550.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            103032..104015
                     /gene="zntB"
                     /locus_tag="ACHJ7J_000265"
     CDS             103032..104015
                     /gene="zntB"
                     /locus_tag="ACHJ7J_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO4998551.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            104290..104463
                     /gene="ynaL"
                     /locus_tag="ACHJ7J_000266"
     CDS             104290..104463
                     /gene="ynaL"
                     /locus_tag="ACHJ7J_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO4998552.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            104493..105866
                     /gene="dbpA"
                     /locus_tag="ACHJ7J_000267"
     CDS             104493..105866
                     /gene="dbpA"
                     /locus_tag="ACHJ7J_000267"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO4998553.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSIELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIATLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(105995..106930)
                     /gene="ttcA"
                     /locus_tag="ACHJ7J_000268"
     CDS             complement(105995..106930)
                     /gene="ttcA"
                     /locus_tag="ACHJ7J_000268"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO4998554.1"
                     /translation="MQENQKNTKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(106982..108217)
                     /gene="intR"
                     /locus_tag="ACHJ7J_000269"
     CDS             complement(106982..108217)
                     /gene="intR"
                     /locus_tag="ACHJ7J_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4998555.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRHSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVTMLNARLS"
     gene            complement(108219..108434)
                     /gene="xisR"
                     /locus_tag="ACHJ7J_000270"
     CDS             complement(108219..108434)
                     /gene="xisR"
                     /locus_tag="ACHJ7J_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO4998556.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            complement(108534..108722)
                     /locus_tag="ACHJ7J_000271"
     CDS             complement(108534..108722)
                     /locus_tag="ACHJ7J_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO4998557.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            complement(108715..108909)
                     /gene="ralR"
                     /locus_tag="ACHJ7J_000272"
     CDS             complement(108715..108909)
                     /gene="ralR"
                     /locus_tag="ACHJ7J_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO4998558.1"
                     /translation="MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYSGSEK
                     EALARWNKRITGNNNGGVHV"
     gene            complement(108974..110026)
                     /locus_tag="ACHJ7J_000273"
     CDS             complement(108974..110026)
                     /locus_tag="ACHJ7J_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecT family recombinase"
                     /protein_id="HFO4998559.1"
                     /translation="MNELTQQENINSNVAVFSPQSLAAIQTFSQVMASGMATVPEHLR
                     GNPSDCMAITMQAMQWQMNPYAVAQKTFVVNGVLGYEAQLVNAVISTRGPLTGRIEYD
                     WFGPWEKIIGKFEIRKSDKGKEYRVPGWKLADENAIGVRVQATLRGESKPRVLELLLA
                     QARTRNSTLWADDPRQQLAYLALKRWARLYCPEVILGVYTQDELDEPQEKIINPVQEH
                     KNTSACCAERETTIIEQDAGENWIDAFRERIEQAQSTEETTALRQEVEAHKNTLGALY
                     TELKGKVVQRHHRLNAIARIEKMINDLPSSGDPEAEQKFTALENTLNAVRPHLGELYE
                     AYKTTLTDLKPEYIGS"
     gene            complement(110038..113181)
                     /gene="recE"
                     /locus_tag="ACHJ7J_000274"
     CDS             complement(110038..113181)
                     /gene="recE"
                     /locus_tag="ACHJ7J_000274"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO4998560.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWESDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHPQD
                     NAQETKELAGGQEENAQADAHGDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQREHITALEMDQENSYIQNLLLAIRGMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKDFISAWLDTEHIDRGLLTKEWRKGNHVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIKEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEDIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKPQPSGTTADEQGEAETMEPDATEHHQDTQPLDAQSQVNSADAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVEGIRDPNDPKWVKGIQTRDSVYQN
                     QPETKQNTPETVKTSPDVKQPESVVQQKQEIVCNACGQTGGDNCPDCGAVMGDATYQE
                     TFDEENQVEAKENDPEEMEGAEHPHKENAGNHPHHDCSDETGEASAHVATEIMWPSYF
                     EPGRYENLPNEVYHSANGISSTMLKDARISLMYYHGRHIAGTIPNEESDALLRGRIIH
                     SYVLETDKFADEYTIPVPVPEYVVTTSNELIAIIKKHNASLPSLMTPELMKEWLESYN
                     STLIQPLSVSAGAEETGILYGSLPEEFRRIPEGEKHTASAMKACIKEYNASLPPLLKT
                     SGTREQLLEQIETVDPELAKKERAKSLPYNISGTKEQLTEIARKIRPELVTLEDWQKR
                     QQEEKAGKTFISPDMYEQAKNIHAALQNNTDAARLLNHPDRKSEISYFGFDEETGLEI
                     RVRPDIEIRLPYESICADVKSVSLGYVRQERLKDRLHREIIERDYHLSAAMYCDVANL
                     DKFFWVFVNKDAGYHWVAVVEASQELLELGRQEYRRTLRQINEALETNNWPAPITESY
                     TDELNDFDLRRLEALSI"
     gene            complement(113281..113554)
                     /locus_tag="ACHJ7J_000275"
                     /pseudo
     CDS             complement(113281..113554)
                     /locus_tag="ACHJ7J_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(113629..113799)
                     /locus_tag="ACHJ7J_000276"
     CDS             complement(113629..113799)
                     /locus_tag="ACHJ7J_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO4998561.1"
                     /translation="MTKKIKCAYHLCNKEIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     SCAEKDQMAHEL"
     gene            complement(113799..114020)
                     /gene="kilR"
                     /locus_tag="ACHJ7J_000277"
     CDS             complement(113799..114020)
                     /gene="kilR"
                     /locus_tag="ACHJ7J_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO4998562.1"
                     /translation="MIVHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDCMIKVFLGRDGLPVKAESW"
     gene            complement(114441..114593)
                     /locus_tag="ACHJ7J_000278"
     CDS             complement(114441..114593)
                     /locus_tag="ACHJ7J_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4998563.1"
                     /translation="MDTIDLGNNESLVYGVFPNQNGTFIAMTYTKSKTFKTEAGARRW
                     LIKNTS"
     gene            complement(114862..115506)
                     /locus_tag="ACHJ7J_000279"
     CDS             complement(114862..115506)
                     /locus_tag="ACHJ7J_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4998564.1"
                     /translation="MESLGIRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSALLTPECKIVAVAKGVQFNL"
     gene            115604..115831
                     /locus_tag="ACHJ7J_000280"
     CDS             115604..115831
                     /locus_tag="ACHJ7J_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4998565.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSRGKLSLNPELYKKKDTSQGEGKGDS"
     gene            115828..116265
                     /locus_tag="ACHJ7J_000281"
     CDS             115828..116265
                     /locus_tag="ACHJ7J_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4998566.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            116352..117158
                     /gene="ydaU"
                     /locus_tag="ACHJ7J_000282"
     CDS             116352..117158
                     /gene="ydaU"
                     /locus_tag="ACHJ7J_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003915366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO4998567.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEADVQTKQERNLTGVQTDVEVVFEHDVNTKATNKDTDKDLKTDPPLNPP
                     RGNRGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRTEQGANGAIRELEKFRQQ
                     GFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            117165..117911
                     /gene="ydaV"
                     /locus_tag="ACHJ7J_000283"
     CDS             117165..117911
                     /gene="ydaV"
                     /locus_tag="ACHJ7J_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4998568.1"
                     /translation="MKNIATGDVLERIRRLAPSHVIAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSAMHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            117934..118686
                     /locus_tag="ACHJ7J_000284"
     CDS             117934..118686
                     /locus_tag="ACHJ7J_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4998569.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTSDELATL
                     FGTTSRKVASTLAMAISKGRMIRVNQNGKFRYCIPGDNLPAEPKAASVTENDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETQADDMVLPSLHMANRELRRAKSH
                     VQKWERVCAALRELNKHRDIVRQMNCSGEDVK"
     gene            118683..118997
                     /locus_tag="ACHJ7J_000285"
     CDS             118683..118997
                     /locus_tag="ACHJ7J_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4998570.1"
                     /translation="MRWRGWVWAEILILRQCAGTMKVKIIGSLIGRSEAAVRTKAREM
                     GISLILRGDFHQSAKYLQRDIELVRQLHQRGVPRREIARKFGIPLRTVNNYVYFDRRV
                     SA"
     gene            118994..119221
                     /locus_tag="ACHJ7J_000286"
     CDS             118994..119221
                     /locus_tag="ACHJ7J_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998571.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLSCPGISASR
                     AGVFFMKTKLYGSTAWIKKAYRVALQEVNSE"
     gene            119214..119522
                     /locus_tag="ACHJ7J_000287"
                     /pseudo
     CDS             119214..119522
                     /locus_tag="ACHJ7J_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            119527..119856
                     /locus_tag="ACHJ7J_000288"
     CDS             119527..119856
                     /locus_tag="ACHJ7J_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4998572.1"
                     /translation="MNQINCQALREAAEAIKIAATPQKLLAFRMKATPQVVLALLDEQ
                     EARDKRITEMEVREVQLSTCYEPRYGHPINTDELCVMIPREHGRWIYLADLEHALRVA
                     GIRIKGQ"
     gene            119858..>120188
                     /locus_tag="ACHJ7J_000289"
     CDS             119858..>120188
                     /locus_tag="ACHJ7J_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998573.1"
                     /translation="MEITPEDESWNIVLFPVKDDDPRNQVNFLYEPSEKPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVKQLRQEERERRKNIEKLIQIE
                     RNVKARIR"
BASE COUNT        31677 a        27892 c        29970 g        30649 t
ORIGIN      
        1 ttatttcatt gcgaaattca tttcgcgatg cgggttcgtt ataactgaat atccctgaca
       61 tatcctcatt aattttaatg aggagggtat ttctcaccct ggagaaaata atgaagtata
      121 tagcaacatc tgtagtggca atgctgctct tatctggttg tgataatacg caaagtaaca
      181 attcatcccc gtcagaaaca gaagtcggcg ttgttacagt caaatctcaa ccggtctcgg
      241 tagtcagtga attaaccgga cgcaccagtg ctgcgctcag tgccgaagta cgtccgcagg
      301 ttgggggaat tatccagaaa cgcttattta aggaaggtga tctggtcaag gctggacagc
      361 cgctctacca gattgatgcg gccagttatc aggctgcatg gaatgaagct cgggcagcat
      421 tacaacaagc acaggcactg gtaaaagccg attgccagaa agcgcagcgt tatgcccgac
      481 tggtgaaaga gaacggtgtt tcacaacagg atgctgatga tgctcagtct acctgtgcac
      541 aagataaagc cagtgtagcg gcgaaaaaag ccgcactgga aactgcgcgc attaatcttg
      601 actggaccac ggtaaccgca ccgatttcag ggcgcattgg catttcgtcg gtaacccctg
      661 gcgcactggt gaccgcgtcg caagatacag cgttaacgac tattcgtggt ctggatacaa
      721 tatatgtcga cctcactcgc tccagtgtcg atttattacg tctgcgtaaa cagtcactgg
      781 cgaccaacag tgacaccatg agcgtctcac ttattctgga agatggcaca acctacagcg
      841 aaaaagggcg tctggaactt accgaagtcg ctgtagatga gtctaccggt tcggtgacat
      901 tacgggcgat tttccccaat ccacaacagc aattattacc gggaatgttt gttcgcgctc
      961 gtgtcgatga aggcgtgatg gaagacgcta ttctcgcgcc gcaacagggc gtcacgcgcg
     1021 atgctaaagg caatgcaact gcgctggtgg tgaataaaga caataaagta gagcagcgaa
     1081 cgctcgaaat gggagaaaca tatggtgata aatggctggt gctaaacggc ctgcacagcg
     1141 gcgaccgact gattgttgaa ggttctgcaa aagtcacttc agggcagacg gtcaaggctg
     1201 ttgaagttca ggctaatgga ggcaacgcct gatgttttcg cgcttttttg ttcgccgccc
     1261 ggtctttgcc tgggttatcg ccattttgat tatgttggcg gggattctgg ctattcgcac
     1321 attgcccgtc gcgcaatatc ctgacgttgc gccaccgacc attaaaattt cagccactta
     1381 tactggtgct tctgccgaaa cgctggagaa cagcgtgact caggttatcg agcaacaact
     1441 cactgggctt gataatttac tctatttcag ctcaaccagc agctctgatg gttcggtcag
     1501 tattaatgtg acctttgaac aaggtaccga tccagatact gcacaggttc aggtgcagaa
     1561 taaaattcag caggcggagt cgcgcctacc cagcgaagtg cagcaaacgg gtgttacggt
     1621 ggagaaatca caaagcaact ttttgctgat tgctgccgtg tatgacacca ctgacaaagc
     1681 ctccagttcg gatatcgccg actggttggt tagtaacgtt caggacccgc tggcgcgtgt
     1741 tgaaggtgtg gggagtctgc aagtctttgg cgcggaatac gctatgcgca tctggcttga
     1801 cccggccaaa ctggcgtctt actcgctaat gccttcagac gtgcaaagtg ctattgaagc
     1861 gcaaaacgtg caggttactg ccgggaaaat aggggcattg ccttcaccga atactcagca
     1921 actgaccgca acggtacgtg cgcagtctcg cttgcagacg gtggatcagt tcaaaaatat
     1981 tatcgtgaaa agccagtcag acggcgcagt tgttcgtata aaagatgtgg ctcgcgttga
     2041 gatgggcagt gaagattata ccgctatcgg caaacttaac ggtcacccgt ctgccggggt
     2101 tgctgtaatg ctttcgcccg gtgcgaatgc gctgaatacg gcgacgctgg tcaaggataa
     2161 gattgccgaa ttccagcgga acatgccgca gggatacgac atcgcttacc cgaaagacag
     2221 cactgaattt attaaaatct ctgtagagga cgtaattcag acgctgtttg aagccatcgt
     2281 attggtggtt tgcgtgatgt atttattcct gcaaaacctg cgtgccacac tgattccggc
     2341 gttagctgtc cccgtcgttt tgctgggcac attcggcgtt cttgcgttgt ttggctattc
     2401 gattaatacc ctgacgttgt ttgcaatggt gctggcgatc ggcttgctgg tggacgatgc
     2461 cattgtggta gtggagaacg tcgaacgcat tatgcgtgac gaagggttgc ccgcgcgtga
     2521 agccacggaa aaatcaatgg gcgagatttc tggcgcactg gttgccattg cgctggtgtt
     2581 gtcagccgta ttcctgccta tggccttctt tgggggatct acgggggtaa tttatcgtca
     2641 gttttcgata accattatct ccgcaatgct gctttccgtg gtggtggcat tgaccttgac
     2701 tcccgccctg tgcggttccg tcctccagca tgttccgcca cataaaaaag gctttttcgg
     2761 cgcatttaac cgcttttacc gccgtactga agataaatat cagcgaggcg taatttatgt
     2821 cctgcgccgt gctgctcgaa cgatggggct ttatctcgta ctgggtggtg gaatggccct
     2881 gatgatgtgg aaactgccgg gcagtttctt acccactgaa gaccagggcg aaatcatggt
     2941 gcagtacacg ctgccggccg gggcgaccgc tgcccgtaca gcagaagtga atcgccagat
     3001 tgttgactgg ttcctgatta acgagaaagc aaataccgat gtcattttta ccgttgatgg
     3061 tttcagtttt agcggcagcg gacagaacac cgggatggcg tttgtttcgt tgaaaaactg
     3121 gtctcaacgt aaaggggcag aaaacaccgc ccaggctatc gccctacggg caaccaaaga
     3181 gctgggcaca attcgtgatg ccacggtatt tgcgatgacg ccgccagccg ttgatgggct
     3241 ggggcaaagc aatggtttta cgtttgaatt gttagctaac ggtggaaccg atcgcgaaac
     3301 actactgcaa atgcgtaatc agttgataga aaaagcgaat caaagtccgg agttacattc
     3361 tgttcgtgcc aatgatttgc cacaaatgcc gcaattgcag gtagatattg atagtaataa
     3421 agcggtgtca ttagggttga gtttgaatga tgtcaccgac accctgtcca gtgcgtgggg
     3481 cggtacttat gtgaatgact ttattgaccg tggtcgtgtg aaaaaagtct acattcaggg
     3541 cgacagtgaa tttcgttccg cgccgtcaga cttaggtaaa tggtttgtgc gcggtagtga
     3601 taacgccatg acaccattct ctgcttttgc gaccacccgc tggctgtatg gaccggaaag
     3661 actggtgcgc tacaacggtt ctgcagccta tgaaattcag ggggaaaatg cgactggctt
     3721 tagttccggc gatgcaatga caaaaatgga ggaactggca aacagtcttc ctgcgggaac
     3781 aacctgggcc tggagtggtt tgtcattgca ggagaaactg gccagtggtc aggcattgag
     3841 tctgtatgca gtttctattc tggtggtttt cctctgcctt gcagcactgt acgaaagctg
     3901 gtcagtcccg ttctcggtca ttctggtgat ccctctgggg cttcttggcg cggcgctggc
     3961 ggcctggatg cgtgatttaa acaacgatgt ttacttccag gtggcgctat taaccactat
     4021 cggcttgtcg tcgaaaaacg ccattctgat tgtggaattt gctgaagcgg cggttgcaga
     4081 gggctattcc ttgagtcgtg cggcattacg cgcggcgcag actcgtttac gcccaatcat
     4141 catgacctcg ctggcgttta ttgcgggggt aatgccgctg gcgatagcaa ccggcgcagg
     4201 ggcgaacagc cgcatcgcca ttggtacggg cattattggc ggtacgctga ccgctacgtt
     4261 gctggctatt ttctttgttc ctctgttttt tgtactggtg aaacgtttgt ttgccggtaa
     4321 accgcgccgt caggagtaag ttatgttgcg tcgttctctt atttttctgg tgctgttgag
     4381 tgcgggatgt gtctcgctcg atccccacta cagcacgcct gaatccccaa tcccggcgac
     4441 cttgccggga gcacagggcc agggaaaggc aatcagccat gactggcagc aggtgatcca
     4501 cgatcaccga ttgcagcagg tggtgactat tgcactgaac agtaaccgcg atgtgcaaaa
     4561 agcgattgct gatattgact cagcacgggc gctctatggg caaactaatg cgtcgttatt
     4621 cccgacggtg aatgcggcct taagttcaac ccgcagtcgt tcgctggcga atggtacgga
     4681 aaccactgcc gaggctgacg gcacggtgtc cagttttacg ttagatctgt ttggtcgtaa
     4741 tcagagttta tctcgggcgg cgcgagaaac ctggcttgcc agtgaattta cagcccagaa
     4801 cacccgttta acgctgattg ctgaaatcag caccgcctgg ctgacactgg cggccgataa
     4861 cagcaacctg gccctggcaa aagaaacgat gaccagtgca gaaaactcat tgaaaattat
     4921 ccagcgccag caacaggttg gcacggcggc agcgacagat gtcagtgagg cgatgagtgt
     4981 ttatcagcaa gcgcgcgcca gcgttgccag ctatcagaca caagtgatgc aggataaaaa
     5041 tgcgctgaac ttactggcag gtacaacgct tgaggaaaat ttgctaccgg gaacactgga
     5101 aagcctgccg gaacagatga taagtctggt tcctgcggga gtgtcatctg acgttctgct
     5161 gcgtcgcccg gatatccagg aagcggaaca taatttgaag agcgccaatg ccgatattgg
     5221 cgcggcgcgg gctaactttt tcccgacgat ttcgctgact gccagcgctg gtgttggcag
     5281 tgacgcattg tcgtcgttgt tcagccacgg aatgcagatc tggtcattta cgccatcggt
     5341 cacattaccg ctgttcactg gcggaagtaa tctggcgcag cttcgttacg cagaagcaca
     5401 aaagcgtgga ctcattgcca cctatgaaaa aaccgttcag agcgcattta aagatgtcgc
     5461 taacgcactg gcgcggcgga ccacgcttga agagcaactg gatgcacagc gccagtatgt
     5521 taaagctgag caacaaacgg tcgatgttgg cttgcgtcgc tatcagacgg gtgttggtga
     5581 ttacctgaca gtgttgacgg cgcagcgcag cttgtggagc gcacagcagg agctgctggc
     5641 actgcaactg actgatttta caaaccgaat cacgctctgg cagtcgctgg gcggcggcat
     5701 gtcatcactc aagtaaggaa taatatggct agagtctctc tttcatgggc attgattctt
     5761 ggtcttttag ccggtatcgg cccgatgtgt accgatcttt atttgccggc tttgccggag
     5821 atgtctgagc aactggcggc aaccacgacc ataacgcaat taacgctgac tgcatcactg
     5881 atcggtcttg gcgtcggaca actgttattt ggccctctga gcgacaaaat agggcgtaaa
     5941 cgtccgttaa tcttgtcgtt gctattgttt attgtttctt ccattttgtg cgcgacaacg
     6001 aacaatattt actggctggt ggtctggcgt tttattcaag ggatcgcggg ggcgggtggt
     6061 tcggtgctct ctcgttctat tgctcgtgac aaatatcagg gagtaacgtt gacccagttt
     6121 tttgcgctgt taatgacggt gaatggcctg gcaccggtgt tgtcgccagt gctgggcggg
     6181 tacattgtta gcacttttga ctggcgcact ttattctggg taatggctga aattagcacc
     6241 gtattgttgc tgggctgcgt gttatttatt aatgagacct tgccagaaaa taaaaggggc
     6301 tcatcattgc tattaaccgg acgaagcgtg gtgcagaacc gccgttttat gcgcttttgc
     6361 ctgattcaaa gttttatgct ggcaggcttg tttgcgtata tcggctcttc gtcgttcgtg
     6421 ttgcagaagg aatttggctt tagtccaatg caatttagcc tggtgtttgg ccttaacggc
     6481 atcggactta tcattgcttc atggatcttt tcgcgtctgg cgcgacggat taatgcgatg
     6541 acattgttgc gaggtggcct gatagcggca atttcgtgtg cattgctcac ggtcttatgc
     6601 gcatggacac aattgcccat tccggcactg gtggcattat ttttcactat tgcattttgt
     6661 agcggcatcg gcactgttgg cggggcagag gctatgagtg cagtagggac acaggaatct
     6721 ggaacggcgt ctgcgttgat ggggatgagc atgtttgtct tcggcggtat agccgcgcca
     6781 ttgtcgggat ttggcggaga aacactgtta aaaatgagtc tggcaattac ggtgtgttat
     6841 acactggcat tcctggttgc tctcaccaga atcgacaatc aaaagtaatg aatacctcat
     6901 cctgaggggg gggccctgtc aggatgaggt gcagaacact aacggtcctt acgccacgca
     6961 tccgccgtca atgcttcgcc aaagtgcccg gcaatcagcc gtttggtgag ttcatgcagc
     7021 ggtgatgcca gcacatccgc ggtgctgcct cgctcgacaa cctcgccctg atgcatcacc
     7081 agcacctggt cgctaatgtg cttcatcatt ccgatatgct gggtaacata aatatacgaa
     7141 atgccctgtt tttcctgtaa ttccagcatc agattaatca actgcgaacg catcgacata
     7201 tccagtgaag cgagggcttc atcggcaata atgacttttg ggcgcaatat cagcgcgcgc
     7261 gccagaccca gacgctgttt ttgcccgggg gccaacatat gcggatagta actgacgtga
     7321 tccggtagca gcccaaccat acgcatcgtt tcaataatct gtttgcgacg ctgttccggt
     7381 tccaggttgg tgttcaggcg cagtggaaaa tccagaattt gcgagatacg ttgacgggga
     7441 ttcaacgagg tcgaaggatc ctgaaaaatc atgcgaatac gctgactgcg gaaggaataa
     7501 tcgccaaaat gcagtggatg atcgtcaatc aataactcgc cgctggtagg ttctatcatc
     7561 cccgccagca tttttgccag cgtggattta cccgaaccat tctcgccaat aatcgccagt
     7621 gtctggcctt cacgtagcgt aaagctcaag ggttttaccg cttctacggt ctggcgacga
     7681 aaccaaccag tccggtagcg gaacgtttta cttaaattac gcacttcaag cagcgtttcg
     7741 atcatctcac tctttctcca tgttcagcgg gaaatgacag gcatagagat gatttttcgc
     7801 ccccgtcaaa cgtggcgtca caatgcattc tcgttgtgca tacgggcaac gtggccccag
     7861 acgacaacca atcggtaact gttccagcag tgggatagcg ccgggcagcg tattgaggcg
     7921 acttttatgc ggcatcgcgc tgccgaagtc tggtatcgcg cggatcagcg cctgggtata
     7981 aggatgatgt ggcatcgtca ccaactcctt acttggcgcg gtttccaccg tttgaccgca
     8041 gtaaagcacg ttaattttat ccgcccattg gctaagcatt tgtaagtcat ggctgataag
     8101 caaaatagtg gtattgctgt tttggttgag acgcgtcagc aggcgaaaga tttgcgcctg
     8161 ggttgttggc tccattgagt ttgtcggttc gtcagcaatc agcagacgcg gttgattcgc
     8221 cagtgcaatg gctatcatca ctttctgaca ttcaccttcg gtcaactcat agggaaaact
     8281 gcgcatcgca tctttgtgat ctttaattcc cacgcggtgc agcagttcaa tggcacggcg
     8341 tttgcgccag ccaaaacgct gccaccaacg gcctttatag gtccaggctg ggatgttttg
     8401 catcaactgg cgacccacac gttctgaagg gtcaagacac gactgcggtt cctggaaaat
     8461 catcgacacg ttgtggccaa ccagtttgcg ccgttcgcgt gcggagagac gcagcaaatc
     8521 gatatcatca aaacgcatac ggtctgcagt aacacgccag ttatctttat tcaccccaca
     8581 aatcgctttc gcaatcaaac ttttgccgga accggattcg ccaacaagac cgcggatttc
     8641 accttcggtt aaggtcatac ttacccggtc aacggcttta acccactcat caccggtttt
     8701 aaattcaata gtcaggttac gaatatcgag taatggcatt attccacccc cgcaataatt
     8761 gcacgacgga caccgtcgcc aaggaggtta accaacagca cgctaatcat aattgccgca
     8821 cctggcagca tgacagtcca cggggcgaca taaatcagtt ccagcgcatc accgagcatc
     8881 gctccccatt caggcgaggg gagttgtgcg ccaagatcga gaaagcccag cgcggcgata
     8941 tcgagaattg ccatcgacag tgcgcgggtg atctcggtga ccagcccggc ggtgatgtta
     9001 ggcatcacag caaaccagag aatattcagc gttgatgcgc catccagacg ggcggcgata
     9061 acgtactctt tttccagttc gtcatgcacc atgctgtaaa tcgaacgtac catacgcggc
     9121 agcagcgcca gccagacggc aaacatggcg tgggacaaac tcggtccggc aaacgctaca
     9181 acgataattg ccaacagtag cgaagggatc gccagtaagg tatccagaat atggttaagc
     9241 accgctgagc ggaggccgtg tgtcgccccg gcgaaggtgc ccagtaccag gccacagatc
     9301 gtcgcggcaa gtgtcaccac aaatgcgcca cccacggttg gcgcagctcc gctcagtaaa
     9361 cggcttaaaa catcgcgtcc gaggtcgtca gtccccagga agaaagaaac ttcgccatag
     9421 cgcgaccatg atggcggcaa taattggtaa ccgagaaatt gctggtcgat gccgtaaggc
     9481 gcaaaccagc cgccaaaaat acacagtaca gccagccccg cgcagccgta caggccgacc
     9541 atcgcagagg cgtcactata aaatttgcgc caggcggtac gcagcgtgcc cggcgggcgc
     9601 ttttcgctgt atacgctatc gtaaggcata ccattcctta tgtttcagag ggttagccat
     9661 ggcacccaaa atatcagaaa tcacgttaac aataatgacc agtgagccac acaccatcac
     9721 tccggcggaa atggctgcgt aatcttgctg gcggatggcg ttaattaacc agcgccctaa
     9781 acccggccag ctaaaaacca tttcggtgat catcgccagc gtcagcatgg tagaaaactg
     9841 taagcccagg cgaggaataa ccggcggtaa cgcgttatgc agaacgtggc gacgcaaaat
     9901 agtaaagcgc gacaaaccgc gggtcgccgc cgcttttaca tagttctggt catacacttc
     9961 gatagtgctg atacgcatca ggcggatcac ttcagttgtt ggcgcaaccg acagggtgat
    10021 cacgggcaat atcatatggc ggattgcgct catgatcatt tcatcccgcc atggtgagtc
    10081 cgaaagccag gcatcaatca gcgcaaaacc ggtaatcggt ttcacttcgt agagcagatc
    10141 gaaacgccct gaaacgggca accaacctag cgtgagtgaa caaaacagcg tcaacagaag
    10201 cgccagccag aacaccggga ttgaaaagcc cagtaaggcg atggcgttga tcaagttatc
    10261 ctgccactta tggcgcgtaa tcccggcaat catccccacg gggatgccaa caatcagcgc
    10321 aaaaccgaag gcgaggatgc acagttccat cgtcgccggg aagacctctt ttaactgttc
    10381 ggcaattggt tgaccgttaa tgctggaaac accaaaatcc cagtggatca ggccattaaa
    10441 ccagaacacc caggcattcc acagtgacgc gccttgtaac ggcgcgtgtg gagtgaaata
    10501 gctcaaactg aagccaacga aggtcagcag gaacaaggtg acaatcaata acaaaatgcg
    10561 gcgtaaggtg aagataatca tggttttttc acctcatcct gtttctcgcg atacacccca
    10621 gcaaaggagg cgttaccaaa cgggctaagt accagacctt tgatatcgta ccgataggcc
    10681 tgcaaacgca atgacgacgc cagcggcaaa atgggcaatt cctgcgccag aatactctgc
    10741 gcttcgtcat aggcttcaat acgcgccgcc agctgctgcg aggagagcgc cttacgcaac
    10801 acgctgtcga atttcggatc gcaccagtgg gcgaggttgg tctgtgaatg aattgccgcg
    10861 cagctcagta acggacggaa gaaactgtcc gggtcgttac tgtccgtcgc ccaaccggat
    10921 aacgtcagat catggctcat atccatcaac cgcgcctcct gaaagcgacc ttctaccggc
    10981 acaatcacca cttttacgcc aacctgcgcc atatccgcct gaatcagttc ggcagttttc
    11041 agtggactgg ggttccacgc ctgcgaacgt gtgggcaccc acagtttcag cgttaaattt
    11101 tccagcccca acgacttcaa ctgttcgcgc gatttcgccg gattgtattc agtaatttta
    11161 gcctcgttgt cataggccca cgaggcgcgc ggtaaaatag aggccgccgt ttcagccgta
    11221 ccataataga tggattgcat caggcgctgg ttattaatcg ccagtgccag tgcatggcgg
    11281 acagcgggat tatttagcgg cggtttggcg gtgttaaatg ccagataggc gacgttcatc
    11341 ccaggacgca gcgttaaacg caagcgcggg tcgtcacgca aaatggatag ctggctggca
    11401 gcaggccagg ccagaacgtc gcattccccg gtcaggagtt tcgacagacg tccggtgccg
    11461 ccggagccta aatccaccac cacctgcggc attaacggtt taccgcgcca gaagtcatca
    11521 tgacgttgta ggcgaataaa ttgcccggcg cggtattccg acaactgata cggtccagtg
    11581 ccgaccggtt gacggtcgag ttgctcctgg cgatcttctt tctctaactt ccgggcatat
    11641 tctgccgaca tgaccgaagc ataatgggtt gcgaggtgcc acaaaaaaga agcatccggc
    11701 tgagccagac ggaactcaac ggtatgatta tccagtttac ggacgctttt cacgttatcg
    11761 gcaaattgca ggctgtcgaa gtaggggaag ttgctgccgt tgacgttatg ccacgggttg
    11821 ttgcggtcaa aaattcgctg gaaggtaaac accacatcgt cggcattcat tttacgagtg
    11881 ggagtaaacc agtcggtttt ttgaaacgga acatcgcgac gtagatggaa gcgataggtc
    11941 gcgccgttgt cgagtacttc ccagctttcg gcaagttctg gcatcaggcg ataggtatag
    12001 ggatcgacat ccagcagtcg atcataaaac tgggcggcaa gggtatcgac aattaaccca
    12061 ctgctcgctt tggatgggtt aaaggtgttg acttgcccgc tgacgcaata gacaaaaccg
    12121 ctgtcgcgga tatcagcatg cgggggggat tcaggcgcgg cgattgcctg accactcaca
    12181 agtccagcaa tcaccaaaag agacgataat acctggcgca taatattaag ggattttatg
    12241 taaagaggct atcttactaa tatttaatga catttgccat taccgtttgt gttcaggggt
    12301 cgtaatgagg gccgtgtggt tggtcgttga acaggtggcg tcgccatctg ttcaacattc
    12361 gtacccgtga tttctctata actataactc acagaacaac ttagcgagga gcagagcggt
    12421 acgatcgtga gaaacccatt aaagcacgct gcagtgcgtg tcattgttag ccggatgcgg
    12481 cgtgaacgct ttatccggac aacgatactg accgatcgtc tgcaattaaa taattactca
    12541 ttaccccatt gattcaaaaa ctctgcgatc tcatcaatgc gtacgggatt aatccccgct
    12601 tcagcagcca tttcatgttg ggcttcttcg ctgatctctt cattgttcat caaacgggtg
    12661 agcagtaact ggaagtagtg ggccagcggt gtgcgttcag cctctgcgac aggctctgcg
    12721 cattcggtcg cgtactcatc ggcaatatca aaatatttta gcgggatatc gtggttcatt
    12781 atttgcccct ggggttaatg cgctaagcat aagggcggat gatagcactc gtgatccggg
    12841 tcatacatct ttttatagtt aatggcgcgg tgcattaccc tcaggcagga taatgcgccg
    12901 cgcatccgac tattacgttt cgctcaccgg cgttctgtca ccatcataca acggcacttt
    12961 gcgatagcgg cggatcagat accataaata tgcgccgcct aaagaagccc ataccagacc
    13021 gagtgttagt gacgttgact cgaggttaac ccacagcaca cccaccgtca gcgcgccaac
    13081 cagcggcatc agcaaatagt ggaagtgatc cttccagctt ttattcattc ctttacgccg
    13141 ccagaaatga ttaaacaccg acagattcac gaaggtaaac gccaccagag caccgaagtt
    13201 aattaacgct gtggcggtaa ctaaatcgaa gaacagcgcc gacaacgcga caatcccgac
    13261 cataatgacg ttcagtgccg gagtccgcca ttttgggtgc acatagccaa acacgcgctc
    13321 cggaaacaca ttgtcacgcc ccattacata cagcaggcgc gacacgctgg catgtgaggc
    13381 caggccagac gctaacgtgt tcacaaacgt ggtgcagagg aaaatcgact ggaagagctt
    13441 gccgccgacg tacagcgcaa tttcaggcag tgcggcgtcc ggatctttaa agcggctgat
    13501 atcgggaaag aacagctgca taaagaacga cgccgcgata aagataacgc caccgtagac
    13561 cgccgtcagg aagatggctt tcgggatcac gcgtgcggcg tccggcgtct cttccgaaag
    13621 cgtggtcacc gcatcgaaac cgaggaacga gaaacagaca atcgtcgccc cggtaataat
    13681 cgggatcagg tgcgcgttct cgctgataaa cggctgaagt gaccagacgg tgccaacgcc
    13741 ttctccttta tgcagtccct gaactaccag gaagataaac accaccatga tggagatttg
    13801 caccagtaca aacagggtat tgaagttagc gaccaggttg acgctcttca gattcgcggc
    13861 ggttaaaatg gcgacgaagg ttactaccca cacccacggc ggcacttccg ggaagagggc
    13921 ggagagatag attttcgcca acaaaacgtt gatcattggc aaaaagagat aatccagcag
    13981 tgatgaccag ccgaccataa atccgacgtg cggattaatc gacttttgcg cgtaggtata
    14041 ggccgaaccg gcctccggaa actggcgaac cagtttgccg tagctgatag cggtaaacag
    14101 cacgcccgcc agcgccagca aataggacgc cggaacgtga ccgtcgctaa tgcctgacac
    14161 aatgccaaaa gtatcgaaca cagtcatcgg cgtgagatag gccagaccca tcattaccac
    14221 ctgccacaat ttcagtgatt ttcgcagacg ggttttgccg ggttgcgcag caatattcag
    14281 tggtgaatta atagccataa ttgctgcctc ccctgcgtaa taaggtttgc ggtcgtgtcg
    14341 acctgaaatc aaacctgttt agcaagggaa gcaaaggggg cggagtccag tcagtcgcag
    14401 gaggggcgca tacgcagcct ccctcgccat gcggggattt aatcatcggc atcatctcat
    14461 ttcctcgtca cagtcttaat cgttaacgga agcccgttgc cgcctgcgct ccataacgcg
    14521 acatccggga aatgtctgga ttaccagcaa atgtcaggcc tggctccgct caggccgatg
    14581 aaacaacccc gcaaggggta ctatgcgttt ttcaacatcc actcaatttc tgtttctgtg
    14641 atgaggcgct cgaactgcaa cagctcatca tttttacagg cgtgatagac atggcaaaac
    14701 cgttcgccta aatagcggcg caggtgatca ttctcgataa actcacccag ggcatcgctc
    14761 tggcgaatcg ggaagggtaa gccatcctgt tccagcccgt tgccttcgac ttcttcctgc
    14821 aacggaagct cgttatcaag accgtgcaaa ataccggcaa aaatcgctgc cattaccaga
    14881 tatgggttgg catcggcacc cgccacgcga tattccacgc ggtgattatg gcgatcgccg
    14941 catggaatac gcagggcgac ggtgcggttg ttatggcccc acgacgcctg cgtcggtaca
    15001 tacatccccg gctggaagcg gcgatacgag ttcacgtttg gtgccagcaa cgccattgat
    15061 gacggcatca gatcgatcat tccggcgagc atttttttca gcagcggcga atcttcgcct
    15121 tccgcgtcag aaagcacgtt ctcgccacga ttattttgca tactgatatg gatatgcatc
    15181 ccgctgcccg cgtgctcttc atacggcttc gccataaaag tggcgtgcat cttatgcttt
    15241 tctgccatca gacgcaccag gcgttttagt gccagcgcat catcgcaggc ttccagcacg
    15301 ttatcggtat ggtaaagatt gatttcaaac tgacccggcg aggcttcggc gaccgcaccg
    15361 tcggcgggaa ttaactgtaa ctgcgccagt tcatcaatat cattgagcac atcggcgaag
    15421 tggttgaggt tatcaaccga gtaaacctgg ctttgtgtat tgcggtcatc ggtgccgggc
    15481 gcgcagggcg gctgcagata cccttcagcg tcgcgctggc gatccagtaa atagaactcc
    15541 agctctaccg ctacgaccgg gaacaatccg cgctggcgca gctgctgcca caggcggtta
    15601 agaacgttcc gcggctcaac gtcaaaggga gcgccatctt catcgaccat ggtcaggagc
    15661 atctgaccga taaactctgg atcggcggca gaaggggtta aggaaccgag aacaggaaca
    15721 caggtacgat ccggctcgcc catttcctga cccagacctg cttcttctac tacgttgccc
    15781 agaatatcca ttgcaaatac cgaggccggg aaataacacc ctttctcgag cttcttcagg
    15841 cttgaaacag gaatacgttt tccacggaag caaccattca aatcggtaag taaaacatca
    15901 acatattgcg tattcgggta gcgctccagg tagcgtttca cttcctgcgt aaaggcgcta
    15961 ccccgcctct cttctgactg ctgaacaaag ttctctactt caacgatatt ggtttccatg
    16021 attcttcgcc tttggtttgt tttccgctcg ttatcaaagc gtaaaatata atgaccacca
    16081 ttcgaatctg tatgcaaact aaatgtttgt caaatgttaa attgagtttg caaaaatgaa
    16141 aacccactgc tagattgaaa aaatattgaa cataaaggtc atttaaaccg cagtaatggc
    16201 gataatttag tccactttgt gagattgagc atggaaaata taatgaacaa tccggttatc
    16261 ggtgtcgtaa tgtgcaggaa caggcttaag ggtcatgcga cccagactct gcaagaaaag
    16321 tacctgaatg ccatcatcca tgcaggcggc ttgccaattg cgctgccaca tgcgctggcg
    16381 gaaccgtcgt tacttgaaca acttttgccg aaactcgatg gcatttacct tcctggtagt
    16441 cccagcaatg tgcagccgca cctatatggt gaaaacggcg atgagcctga cgccgatccc
    16501 gggcgtgatc ttctgagcat ggcgttaatt aatgccgcac tcgaaaggcg catccccatt
    16561 ttcgccatct gccggggttt acaggaactg gtggtggcaa ccggtggttc gttgcatcgc
    16621 aagctgtgcg aacagcctga attgctggaa catcgggaag atcccgaact gccggtggaa
    16681 cagcaatatg caccgtcgca tgaagttcag gttgaagagg ggggattact gtctgcgttg
    16741 ttacctgaat gtagcaactt ttgggtaaac tctctgcatg gacaaggggc gaaggtcgtt
    16801 agcccacggt tgcgtgttga agctcgctcg ccggatggct tagttgaggc ggttagcgtc
    16861 atcaatcatc cttttgcgct gggcgtacag tggcacccgg aatggaacag tagcgagtac
    16921 gcgctttcgc gtatattgtt cgagggcttt atcaccgctt gtcagcacca tatcgctgaa
    16981 aaacagcgac tctgaccact acagtttaag gaaatgcaaa tatgagtgat gagggactgg
    17041 cgccaggaaa acgcttgtcg gaaatccgcc agcagcaggg gctttcacaa cgtcgtgccg
    17101 ccgaactctc cgggctgact cacagtgcta tcagtacgat agaacaagat aaagtcagcc
    17161 ctgccatcag tacgctgcaa aagctgctga aggtgtatgg tctgtcactc tcggaattct
    17221 tttccgagcc ggaaaaacct gatgagccgc aggtcgtcat taatcaggac gatttaattg
    17281 agatgggcag tcagggtgtg tcaatgaagc tggttcataa cggtaacccg aatcgcacgc
    17341 tggcgatgat ctttgaaacg taccagccgg gcacaaccac tggggaaaga attaagcatc
    17401 agggtgagga aataggcact gtactggaag gtgaaattgt tctgacgatt aatggtcagg
    17461 attaccacct cgtcgcgggg caaagttatg ccattaatac cggcatcccg cacagtttca
    17521 gtaatacgtc ggcaggtatt tgccgaatta tcagcgccca tacgcccacc acgttttaat
    17581 ctttttgttc tgtaagccgg gtaagcgcaa tgcgcccggc aatccataaa gaatgtcgcc
    17641 agaaataaac gggcatacgg cccggggagc tctgcgccct gacgttcaca aactgcatat
    17701 atctgataga cgtgaaacag gagtcataat gaattttcat catctggctt actggcagga
    17761 taaagcgtta agtctcgcca ttgaaacccg cttatttatt aacggtgaat atactgctgc
    17821 ggcggaaaat gaaacctttg aaaccgttga tccggtcacc caggcaccgc tggcgaaaat
    17881 tgcccgcggc aagagcgtcg atatcgaccg tgcggtgagc gcagcacgcg gcgtatttga
    17941 acgcggcgac tggtcactct cttctccggc taaacgtaaa gcggtactga ataaactcgc
    18001 cgatttaatg gaagccaacg ccgaagagct ggcactgctg gaaactctcg acaccggcaa
    18061 accgattcgt cacagtctgc gtgatgatat tcccggcgcg gcgcgcgcca ttcgctggta
    18121 cgccgaagcg atcgacaaag tgtatggcga agtggcgacc accagtagcc atgagctggc
    18181 gatgatcgtg cgtgaaccgg tcggcgtgat tgccgccatc gtgccgtgga acttcccgct
    18241 gctgctgact tgctggaaac tcggcccggc gctggcggcg ggaaacagcg tgattctaaa
    18301 accgtctgaa aaatcaccgc tcagtgcgat tcgtctcgcc gggctggcga aagaagcagg
    18361 tctgccggat ggcgtactga acgtagtaac gggttttggt catgaagcgg ggcaggcgct
    18421 ttcgcgtcat aacgatatcg atgccatcgc ctttaccggt tcaacccgta ccgggaaaca
    18481 gctgctgaaa gatgcgggcg acagcaacat gaaacgcgtc tggctggaag cgggcggcaa
    18541 aagcgccaac atcgttttcg ctgactgccc ggatttgcaa caggcggcaa gcgccaccgc
    18601 agcaggcatc ttctacaacc agggtcaggt gtgcatcgcc ggaacgcgcc tgttgctgga
    18661 agagagcatc gccgatgaat tcttagccct gttaaaacag caggcgcaaa actggcagcc
    18721 gggccatcca cttgatcccg caaccaccat gggcacctta atcgactgcg cccacgccga
    18781 ctcggtccat agctttattc gggaaggcga aagcaaaggg caactgttgt tggatggccg
    18841 taacgccggg ctggctgccg ccatcggccc gaccatcttt gtggatgtag atccgaatgc
    18901 gtccttaagc cgcgaagaga ttttcggtcc ggtgctggtg gtcacgcgtt tcacatcaga
    18961 agaacaggcg ctacagcttg ccaacgacag ccagtacggc cttggcgcgg cggtatggac
    19021 gcgcgatctc tcccgcgcgc accgcatgag ccgacgcctg aaagccggtt ccgtcttcgt
    19081 caataactac aacgacggcg atatgaccgt gccgtttggc ggctataagc agagcggcaa
    19141 cggtcgcgac aaatccctgc atgcccttga aaaattcacc gaactgaaaa ccatctggat
    19201 aagcctggag gcctgaaatg accgaacata ccagcagtta ctacgccgcc agtgcgaata
    19261 aatacgcgcc attcgacacg ctgaatgagt caatttcctg cgacgtttgc gtggttggcg
    19321 gcggctatac cgggctgtct tccgcactgc atctggcgga agcgggcttt gacgtggtgg
    19381 ttctggaagc ctcacgcatc ggctttggcg ccagcgggcg caacggcggg cagcttgtga
    19441 actcctacag ccgcgacatc gacgttatcg aaaaaagcta cggcatggac accgcccgta
    19501 tgctcggtag catgatgttt gaaggcggag agatcatccg cgaacgtatc aaacgttatc
    19561 agattgactg cgactatcgt cctggcggtc tgtttgtggc gatgaatgat aaacaactcg
    19621 ccacactcga agagcagaaa gagaactggg aacgctacgg caataaacag ctggagatgc
    19681 tggacgccaa cgccattcgc cgcgaggtcg ccagcgatcg ctacactggt gcgctgctgg
    19741 atcacagcgg tggacatatt cacccgctaa acctcgccat tggtgaagcc gatgccatcc
    19801 gcctgaacgg cgggcgcgtg tatgagcttt ctgcggtgac gcagatccag cacaccacgc
    19861 ccgccgtagt gcgtacagcc acaggtcagg tgacggcgaa gtatgtgatt gtcgccggga
    19921 atgcgtacct gggggataaa gtggagccgg aactggcgaa acgcagcatg ccgtgcggta
    19981 cgcaggtgat caccaccgag cggctatcag aagatttagc ccgttcgctg atcccgaaaa
    20041 actactgcgt ggaagactgt aactatctgc ttgattacta ccgtctgact gccgacaacc
    20101 gcctgctgta cggcggcggg gtagtgtatg gcgcacgcga cccggatgat gttgagcgcc
    20161 ttgtagtgcc gaaactgctg aaaaccttcc cgcaactgaa gggcgtcaaa atcgactatc
    20221 gctggacggg caacttcctg ctgaccctgt cgcgtatgcc gcagttcggt cgcctcgata
    20281 ccaacatcta ttacatgcag ggctacagcg gccacggcgt cacttgtacc cacctcgccg
    20341 ggcgtctgat tgccgaactg ctgcgcggcg acgccgaacg tttcgatgcc ttcgccaatc
    20401 tgccgcatta cccgttcccc ggcgggcgca cgctgcgtgt gccgtttacc gcgatgggcg
    20461 cggcttatta cagcctgcgc gatcgtctgg gcgtttaatt tccgattaac agtgaagagt
    20521 caaaaggtgt gaaacatgag caacaatgaa ttccatcagc gtcgtctttc tgccaccccg
    20581 cgcggggttg gcgtgatgtg taacttcttc gcccagtcgg cagaaaacgc cacgcttaag
    20641 gatgttgagg gcaacgagta catcgatttc gccgcaggca ttgcggtgct gaacacgggg
    20701 catcgccacc ctgaattggt cgcggcggtg gagcagcaac tgcaacagtt tacccacacc
    20761 gcgtaccaga ttgtgccgta tgaaagctac gtcaccctgg cggagaaaat caacgccctt
    20821 gccccggtga gcgggcaggc taaaactgcg ttcttcacca ccggtgcgga agcggtggaa
    20881 aacgcggtga aaatcgcccg cgcccatacc ggacgccctg gcgtgattgc gtttagcggc
    20941 ggctttcacg gtcgtacgta tatgactatg gcgttaactg gaaaggtcgc gccgtacaaa
    21001 atcggcttcg gcccgttccc tggttcggtg tatcacgtag cttatccgtc agatttacac
    21061 ggcatttcaa cacaggactc cctcgacgcc atcgaacgct tgtttaaatc agacatcgaa
    21121 gcgaagcagg tcgcggcgat tattttcgaa ccggtgcagg gcgagggcgg tttcaacgtt
    21181 gcgccaaaag agctggttgc cgccattcgc cgcctgtgcg acgagcacgg tattgtgatg
    21241 attgctgatg aagtgcaaag cggctttgcg cgtaccggta agctgtttgc catggatcat
    21301 tacgccgata agccggattt aatgacgatg gcgaaaagcc tcgcgggcgg gatgccgctt
    21361 tcgggcgtgg tcggtaacgc gaatattatg gacgcacccg cgccgggcgg gcttggcggc
    21421 acctacgccg gtaacccgct ggcggtggct gccgcgcacg cggtgctcaa cattatcgac
    21481 aaagaatcac tctgcgaacg cgcgaatcaa ctgggccagc gtctgacaaa cacgctgatt
    21541 gatgccaaag aaagcgttcc ggccatcgcg gcggtacgcg gtctggggtc tatgattgcg
    21601 gcagagttta acgatccgca aacgggcgag ccgtcagcgg cgattgcaca gaaaatccag
    21661 caacgcgcgc tggcgcaggg actgcttcta ctgacctgtg gcgcatacgg caacgtgatt
    21721 cgtttcctgt atccgctgac catcccggat gcgcaattcg atgcggcaat gaaaattttg
    21781 caggatgcgc tgagcgatta atagcccaac gcaataatgt ctgatgcgct gcgcttatca
    21841 ggcctgcaaa cgacgtattg attatgtatg ccggataaag cattcacgcc gcatccggca
    21901 agttgtattg cccaacttcg ctaaatctgg tgctttttca acaacgcgcg gaactgatga
    21961 taggttaacc ccagcaattc agctgcgcgc ttctggttat atttcccttg ttgcaaactg
    22021 agttgcagca actctttttc ctgctgcatc tgaaactcac gtaaatccag cggcagcatc
    22081 ggaagcgagg tattatctga aacggcgatg gcttcttcag gtggacgccg tttaaaggga
    22141 tcaataatga tgtcatcaag cggataatcg ctggtgccgt ggcgatacac tgaacgttcc
    22201 accacgtttt tcaattcacg aatatttcct ggccaacgat aattcagcaa tgtttctctg
    22261 gcgcgctccg taaaccccgg gaacagaggc agcttgattt cccgacacat ctggatggca
    22321 aagtgttccg ccattaacat aatgtcgctt tcacgttcgc gtagtggcgg cagttgcact
    22381 acatcgaaag ccagccggtc gagcaggtca gcgcgaaaag taccttcatt gaccatcgcc
    22441 gggagatcgg cattcgtcgc gcataccaac cgcacattca cctgcaatgg ctggctgccg
    22501 ccaacgcgct ccagttcacc gtactcaatc acgcgcaata atttctcctg caccatcatc
    22561 ggtgccgtag cgagttcatc aagaaatagc gtaccgccgt cggcacgttc aaatctccct
    22621 ggatgacgtt tttgcgcacc ggtaaacgcc cccgcttcgt gaccaaacag ttcggaatcc
    22681 agcagatttt catttaacgc cgcgcagtta agggaaataa acggcccttg ccaacgggag
    22741 gagagataat gcaggcggct ggcaatcagc tctttaccgg tgccgcgttc gccgatgatg
    22801 agcaccggtt tgtccagtgg tgcgagatgc gaaacctgtt ccagcacttc gagaaagctg
    22861 ttcgcctcac cgagtaaatt atctttgtat tctgccatga tgaaattcgc cacttgttag
    22921 tgtaattcgc taattcatcc tggcatgttg ctgttgattc ttcaatcaga tctttataaa
    22981 tcaaaaagat aaaaaattgg cacgcaaatt gtattaacag ttcagcagga caatcctgaa
    23041 cgaagaaatc aagaggacaa cattatgggt attttttctc gctttgccga catcgtgaat
    23101 gccaacatca acgctctgtt agagaaagcg gaagatccgc agaaactggt gcgtctgatg
    23161 atccaggaaa tggaagacac gctggttgaa gtgcgttcta cttcggcgcg tgcgttggca
    23221 gaaaagaaac agctgactcg ccgtattgaa caagcgtcgg cgcgtgaagt tgaatggcag
    23281 gaaaaagccg aactggcgct gctgaaagag agagaggatt tggcgcgtgc ggcgttaatt
    23341 gaaaaacaga aactgaccga tctgattaag tccctggaac atgaagtgac gctggtggac
    23401 gatacgctgg cacgcatgaa gaaagagatt ggcgagctgg aaaacaaatt gagcgaaaca
    23461 cgcgctcgcc agcaggcatt gatgttacgc catcaggcgg caaactcgtc gcgcgatgtg
    23521 cgtcgtcagc tggacagtgg caaactggat gaagcaatgg ctcgtttcga atctttcgaa
    23581 cgtcgtattg accagatgga agcggaagca gaaagccaca gcttcggtaa acaaaaatcg
    23641 ctggacgatc agtttgccga actgaaagcc gatgatgcaa tcagcgagca actggcacaa
    23701 ttaaaagcca aaatgaagca agacaatcaa taataacatc caggcggcgt ccgaacgcgc
    23761 cgccgctcat cgtctaagga gtacttatga gcgcgctatt tctggctatt ccattaacca
    23821 tttttgtgct gtttgtttta ccgatctggt tatggctgca ttacagcaat cgttctggtc
    23881 gcagtgaatt gtcgcaaagt gagcagcagc gattagcgca actggctgat gaagcaaaac
    23941 ggatgcgcga acgtattcag gcgctggaat ctattcttga tgcagaacat ccgaactgga
    24001 gggatcgcta atggcgggca ttaatctcaa taaaaaatta tggcgtattc cacagcaggg
    24061 catggtccgt ggcgtctgcg ccgggattgc caactatttt gatgtaccgg taaaactggt
    24121 gcgtatcctg gtggtgttgt cgattttctt cggtctggcg ctgtttaccc tggttgctta
    24181 catcattttg tcatttgcgc ttgatccaat gccggacaac atggcctttg gtgagcagct
    24241 accttccagc agtgaattgc tggatgaagt cgaccgtgaa ctggcggcaa gtgaaacgcg
    24301 tttacgcgag atggaacgtt atgtcacttc cgatacattc acgttacgta gccgtttccg
    24361 tcaactgtga ggaaagttat gaatactcgc tggcaacagg ccgggcaaaa ggtaaagcct
    24421 ggtttcaaat tagcaggcaa gctggtactt cttaccgcac tgcgctatgg cccggcgggt
    24481 gtggcgggct gggcgataaa atcagttgct cgccgaccgc tgaaaatgtt gctggctgtg
    24541 gcgctggaac cgctgttaag tcgggctgct aataaactgg cacagcgtta taaaaggtga
    24601 ggggagtttc gcaaaaattg ttaaatctca ggcctataat ggatggcaat tttcatccat
    24661 agaaggacgc ttacatgttt aaaaaaggct tacttgctct ggcactggtg ttttcactgc
    24721 ccgtttttgc cgctgaacac tggatcgatg ttcgtgttcc agagcagtat cagcaagagc
    24781 acgttcaggg ggccatcaat attcccctga aggaagtgaa agagcgcatt gccaccgtcg
    24841 ttccggataa aaacgacacc gtgaaagtgt attgcaatgc cggacgccag tcagggcagg
    24901 caaaagagat ccttagcgag atgggatata cccacgttga gaacgccggt ggcctgaaag
    24961 acatcgcaat gccgaaggtc aaaggttaac aaattactta ttgagcggtg taaacaacgc
    25021 cgcttattca tttgataaat caaaattaac ccacattatt atctccagta tctaatcctt
    25081 tctacagctt tctggcacag gacagcaatg aagcgactta aaaatgaact caatgcgctg
    25141 gtgaatcggg gtgtcgacag acatctgcgc ctcgctgtaa ccggacttag ccgcagcggc
    25201 aaaacagcgt ttatcaccgc gatggttaat cagttgctca atattcatgc cggagcacgc
    25261 ttgccgctgt taagtgcggt gcgtgaagag cgtctgctgg gcgtaaaacg cattcctcag
    25321 cgtgactttg gcattccgcg ttttacctac gacgaagggt tggcgcagct gtatggcgat
    25381 cctcccgcct ggccgacgcc aacgcgcggc gtcagcgaaa ttcgcctggc gctacgctat
    25441 aaatcgaacg attcgctgct gcgccacttt aaggatacct ccacgctgta tctggagatt
    25501 gtggattacc ccggcgaatg gttgctcgac ctgccgatgc tggcgcagga ctatttaagc
    25561 tggtcgcgcc agatgacggg cttactcaat ggtcagcgcg gcgaatggtc ggcgaaatgg
    25621 cgaatgatgt gcgaagggct ggacccgcta gcgcctgccg acgaaaaccg gctggcagac
    25681 attgccgccg cgtggaccga ttatctccac cactgtaaac agcaggggct gcactttatt
    25741 cagccagggc gctttgtctt gccgggagat atggcaggtg cgcccgcgct gcaattcttc
    25801 ccgtggccgg atgtcgatgc ctggggcgag tccaaactgg cgcaggccga taagcacacc
    25861 aatgccggaa tgctgcgcga gcggtttaat tattactgcg agaaggtggt gaaggggttc
    25921 tataagaatc attttctgcg ctttgaccgc cagattgtgc tggtggactg cctgcaacct
    25981 ctcaacagtg ggccacaggc atttaatgat atgcgtctgg cgctgacgca actgatgcaa
    26041 agttttcact acgggcagcg taccctgttc cggcgtttgt tttcgccggt tatcgataag
    26101 ctattgtttg ctgccactaa agcggaccat gtgaccatcg atcagcacgc taatatggtt
    26161 tcattactgc aacaactgat tcaggatgcc tggcaaaatg cggcgttcga agggatcagc
    26221 atggactgcc tggggctggc gtctgttcag gcgaccacca gcggcattat tgatgttaac
    26281 ggtgagaaaa tcccggcgct gcgcggtaat cgacttagtg atggcgcacc gctcactgtt
    26341 tatcctggcg aagtccccgc acgtttgcct ggtcaggcgt tctgggataa gcaaggcttc
    26401 cagtttgagg cgtttcgtcc gcaggtgatg gatgtcgaca aaccgctgcc gcatattcgt
    26461 cttgatgccg cgctggaatt tttaatagga gataaattgc gatgaccgaa ccgttaaaac
    26521 cacgtattga tttcgacggt ccgctggagg tcgatcagaa tcctaaattc agggcgcagc
    26581 agacctttga cgaaaatcag gcgcaaaatt ttgccccggc cacgctcgac gaagcgcagg
    26641 aagaagaggg gcaagtcgaa gcggtaatgg acgcagcgtt acgtccgaaa cgcagcctgt
    26701 ggcgcaaaat ggtgatgggc gggctggctc tgtttggcgc aagcgttgtc gggcagggtg
    26761 tacagtggac aatgaatgcc tggcaaactc aggactgggt ggcgctgggt ggatgtgccg
    26821 ctggggcatt gattatcggc gctggcgtag gttctgtggt aacagagtgg cggcgcttat
    26881 ggcgcttgcg acagcgcgcc catgaacgcg acgaagcgcg tgatttattg catagccacg
    26941 gaaccggcaa aggccgcgca ttttgcgaaa aactggcgca gcaggcgggt attgatcagt
    27001 cgcatccggc gctgcaacgc tggtatgcct caatccatga aacgcaaaac gaccgtgaag
    27061 tggtcagcct gtatgcgcat ttggtccagc cagttttaga tgcccaggcg cggcgcgaaa
    27121 tcagccgttc ggcagcggaa tcaacgttga tgattgcggt cagcccgctg gcgctggtgg
    27181 atatggcatt tatcgcctgg cgcaatctgc gtttgattaa tcgcatcgcc acgctgtatg
    27241 gcattgaact gggatattac agccgtttgc gcctgttcaa gctggtattg ctgaatatcg
    27301 ctttcgccgg agccagcgaa ttggtgcgcg aagtggggat ggactggatg tcgcaagatc
    27361 tcgctgctcg tttgtctacc cgcgcagctc aggggattgg tgcaggactt ctgacggcac
    27421 gactggggat taaagctatg gagctttgcc gcccgctgcc gtggattgac gatgacaaac
    27481 ctcgccttgg ggatttccgt cgtcagctta tcggtcaggt gaaagaaacg ctgcaaaaag
    27541 gcaaaacgcc cagcgaaaaa taatgcaata tcgggtgctg accggatatc tttacgccga
    27601 agtgcccgtt tttccgtctt tgtgtcaatg attgttgaca gaaaccttcc tgctatccaa
    27661 atagtgtcat atcatcatat taattgttct tttttcaggt gaaggttccc atgcgtctgg
    27721 aagtcttttg tgaagaccga ctcggtctga cccgcgaatt actcgatcta ctcgtgctaa
    27781 gaggcattga tttacgcggt attgagattg atcccattgg gcgaatctac ctcaattttg
    27841 ctgaactgga gtttgagagt ttcagcagtc tgatggccga aatacgccgt attgcaggtg
    27901 ttaccgatgt gcgtactgtc ccgtggatgc cttccgaacg tgagcatctg gcgttgagcg
    27961 cgttactgga ggcgttgccc gaacctgtgc tctctgtcga tatgaaaagc aaagtggata
    28021 tggcgaaccc ggcgagctgt cagctttttg ggcaaaaatt ggatcgcctg cgcaaccata
    28081 ccgccgcaca attgattaac ggctttaatt ttttacgttg gctggaaagc gaaccgcaag
    28141 attcgcataa cgagcatgtt gttattaatg gacagaactt cctgatggag attacgcctg
    28201 tttatcttca ggatgaaaat gatcaacacg tcctgacagg tgcggtggtg atgttgcgat
    28261 caacgattcg tatgggccgc cagttgcaaa atgtcgccgc ccaggacgtc agcgccttca
    28321 gtcaaattgt cgctgtcagc ccgaaaatga agcatgttgt cgaacaggcg cagaaactgg
    28381 cgatgctaag cgcgccgctg ctgattacgg gtgacacagg tacaggtaaa gatctctttg
    28441 cctacgcctg ccatcaggca agcccaagag cgagcaaacc ttacctggcg ctgaactgtg
    28501 cgtctatacc ggaagatgcg gtcgagagtg agctgtttgg tcatgctccg gaagggaaga
    28561 aaggattctt tgagcaggcg aacggtggtt cggtgctgtt ggatgaaata ggtgagatgt
    28621 caccacggat gcaggcgaaa ttactgcgtt ttcttaatga tggcactttc cgtcgggttg
    28681 gcgaagacca tgaggtgcat gtcgatgtgc gggtgatttg cgctacgcag aagaatctgg
    28741 tcgaactggt gcaaaaaggc atgttccgtg aagatctcta ttatcgtctg aacgtgttga
    28801 cgctcaatct gccgccgcta cgtgactgtc cgcaggacat catgccgtta actgagctgt
    28861 tcgtcgcccg ctttgccgac gagcagggcg tgccgcgtcc gaaactggcc gctgacctga
    28921 atactgtact tacacgttat gcgtggccgg gaaatgtgcg gcagttaaag aacgctatct
    28981 atcgcgcact gacacaactg gacggttatg agctgcgtcc acaggatatt ttgttgccgg
    29041 attatgacgc cgcaacggta gccgtgggcg aagatgcgat ggaaggttcg ctggacgaaa
    29101 tcaccagccg ttttgaacgc tcggtattaa cccagcttta tcgcaattat cccagcacgc
    29161 gcaaactggc aaaacgtctc ggcgtttcac ataccgcgat tgccaataag ttgcgggaat
    29221 atggtctgag tcagaagaag aacgaagagt aagcgcgaat atgcctgatg gtgcaacacc
    29281 atcaggcata ttaacttatg ctttcagtac agccagagct gcttcgtaat ccggctcggt
    29341 ggtgatttca tccaccagct ggctgaaatt cacattgtca ttttcatcaa taaccacaac
    29401 ggcacgcgct gccagacctt tcagtgggcc atcagcaatt gccacaccgt aggcttgtag
    29461 aaattcagcg ttacggaaag tggagagggt gataacgttg ttcagacctt ctgcgccgca
    29521 gaaacgagac tgggcgaacg gcagatcggc agagatacac agcacaacgg tgttgtcgat
    29581 ctcggttgcc agttggttaa acttacgtac tgatgcggcg caaacaccgg tatcaatact
    29641 tgggaaaatg ttcagcactt tgcgtttacc cgcaaactga ccgagggtga cgtcagacag
    29701 atcttttgcc acgagagtaa aagtctgcgc tttgctaccc gcctgcggga tggaattggc
    29761 gactgtaacc gggttgccct ggaaatgaac ggtttgtgac atgattatct ttcctgttta
    29821 catatagtta acgtcacacc tagtttatgc taactgtcaa taacacagca aacgctattt
    29881 gcgcttaatc cgcagaccac cgcgacaaca aggagtaaag atgagaaccg ttaaggtatt
    29941 tgaggaagcc tggcccttac ataccccgtt tgtgattgcc cggggaagtc gcagtgaagc
    30001 gcgcgtggtg gtggttgaac tggaagaaga gggtattaaa ggcaccggcg aatgcacgcc
    30061 atatccgcgt tatggagaaa gtgatgcctc ggtaatggcg caaattatga gcgtcgtgcc
    30121 gcaattagag aaagggctga cacgggagga gttgcaacaa attctccctg ccggcgcagc
    30181 acgtaatgcg ctggattgtg cattgtggga tctggccgcg cgaaaacagc agcaatcgct
    30241 ggctgatttg atcggcataa cgcttcccga gacagttatc actgcacaga cggtagtcat
    30301 cggtacgcct gatcagatgg ccaatagtgc atcaacactc tggcaggccg gcgcgaaatt
    30361 actgaaagtg aagctggata accatcttat cagtgagcgg atggtggcaa ttcgcacagc
    30421 tgtgcccgat gccacgctga tcgttgatgc aaatgaatcc tggcgtgcag aagggttggc
    30481 ggcacgttgc cagctattgg cggatttagg cgttgcgatg cttgaacaac cgcttcctgc
    30541 gcaggacgat gcggcactgg agaattttat tcatccgtta ccgatttgtg ctgatgaaag
    30601 ttgtcatact cgtagcaatc tgaaggcgct gaaagggcgc tatgagatgg ttaacattaa
    30661 gctcgataaa accgggggtc tgacggaagc gctggcgctg gcgactgaag cacgtgcaca
    30721 aggttttcgt ctgatgctgg gctgcatgtt gtgtacctct cgtgccatta gcgccgcttt
    30781 accgctggtg ccgcaggtca gtttcgccga tcttgacgga ccgacctggc tggcggtaga
    30841 tgtggaaccg gcgcttcagt tcacgacggg cgaattgcat ctttaggatg ccagcgcagc
    30901 aagtttgcca tcgcaaagag atatttttcg ctggcttcgt cggaggagat gggcggaaac
    30961 tctgcggtga tgcaatgcaa gttgagatct gcacaccaac tgccgaaaga gccaggcgtt
    31021 tcataaccga cgctggttac cagaggcaat tcaaacgcct gagccagcca ttcgcctaat
    31081 tcgctgtgtc tgggatcttc aatacaggcc agtggatcgt ggaatgagac cacccaggca
    31141 ggttgaatgc ggtgtataag ctggcacagc gcctgggttt caggttcgga gccaggtttg
    31201 tcaccagtca gtaatacgac atcacgttct tcagcggcgc tgttccagcg ataaaccgtt
    31261 tcaccttcct tccagtttgc cgccggaaag tttctgttta aatccacacc attagcattg
    31321 gcccgtaacc ccagctggca gccgtcagga ttcacacaca gcaccacatg atggcggcgc
    31381 aaagaaggtg tcaaagtccg cagagcacag gagagcgtga cgaccgaaga gttttcatca
    31441 ccgtgagtgc cagccagaat caaaccactt tcgcgactgg ctgcaggggc cggaaaccag
    31501 attaacggcg cgcccaataa tgaacgtccg taatgttctg ttccgggcgg aaatgcgccg
    31561 cgttcggcgc gtgggcgggt tacggtcatg atcgtctctg gttcaggaag tattacaggc
    31621 agtgttgtgc aaattttgcg gattatcaaa tccttttctt caacgctatg tcatcgatct
    31681 tgtatcactt ttttgccgga agttgtttgt atttttattg atgtgcagat attagaattg
    31741 tcgtaatgtt atatacatgg cgtattttat tcttactggt tctacacatg ttaatcgcat
    31801 ctgattgatc agttcacctt aataagttta ataaatcgta ctgcacatag tacagtacga
    31861 tggtggctat ataattgatt tacatgaaga atggcttatc gccatcagtc ccgggttgat
    31921 tgatgcctat attctcatca atgtgtttct caggtttatc gatgatatct gtaattaatg
    31981 ctgcgacact cttacgtgat acgatcgtac ctttgaatgg ttcattgcgt gaggtaagct
    32041 cataatcaat aatatcttca tccgtcagcc aggcagggcg aagaattgta tattccagtc
    32101 cagaagcctc aattgcatcc gcagccctgc ggaatggttt taggggctca ccaataactg
    32161 cattattcca ttcgccaaat tttccaggaa cttcatcata aatgccgagg gaaagaacaa
    32221 agatcagacg tttaacatca caggctttca tcgcagcgat cactgaattt gcctgaatat
    32281 ctaaatcttc ccccgtaagg ttggcataca caatatcctg tccctgcatg gcctgcttca
    32341 gtgctgcatg attcagtaca tcccccataa taatctggct attagttgga tagggtttgt
    32401 gtatttttgc tggctgacga gcaaataaag tttgtttaat ggtttgttta tctgccagtt
    32461 gattaatcac atggcgggct atctgaccgc cagcaccaag aatgagtaca tttttcataa
    32521 tatttctcat gaaacttatc acagatgggt gttgaaaaaa ggaataactt tttccagcgc
    32581 ctcctgaact gcttccggtt tgtcatacag ttcataatgt gagaaatcgg gtaatgaaac
    32641 taactgacga tcctgcgaaa ccatcgcccg gccatagact tccagtccat cacgataagc
    32701 accgaaggca ccgacttttt caccaactac taccatcacc ggctgagtca gtaacacttc
    32761 agtaaatgcg aaggcatccc aggccagcgt tttctgtgca tgtgagaaca acatttttgt
    32821 cgcacctccc ggttgttgcc cgcgtggtgt tttatagtag tcagttgctt cataaacatc
    32881 gcgttcggtc agtccatggg cttttgctgc atccaggctg gcgggaagta attcgttgat
    32941 ctgtaattct ccaccacgag cctctttggt tcgttgcgac gccattgcat tcaatgcgcc
    33001 aatcggatcg taattgctga aaccttcccg aaaaagacgc ccgatattaa cacccgtaat
    33061 actcactaca gctttaattc gtttttctgt cagcgcggca ttaatggcat agccaccccc
    33121 cccacacaca cccagcacgc caatgcgttc agcatcaaca tatggcagag ttactgcata
    33181 atcgataaca cggctaatat cctctacgcg ctgtgtcgga tcttctatcc aacgaggctc
    33241 cccaccagat tctccctgga aactggcgtc ataagcgaga acgacatagc ctttttcagc
    33301 caatgccttg ccatagatat tgccagacgt ctgctcctta caactgccaa aaggatggac
    33361 gctgataacc gccggatact gttttgactc atcaaaattt ggtggaaact gaatattggc
    33421 agcaatatgc caggccatat cactattgcg gatttgaata tgtttcatta cagagtttcc
    33481 tcaaagaacg gagctaatac tgaaattgct tcagccacat aggctttgcc gtcataaaga
    33541 tccatatggt ttgcgccttc aacaatgtga tagcgtttat cctggcttga tgcccgatcg
    33601 tacaggtcgt cactcatcca tttgctccct gcctggctgc ccgccacaat ctgcatcggc
    33661 tgagtcaggt acacttccgc catatggtaa gcatcatagg taataatctg gttaaggctg
    33721 cgcaaagtag cgtaacccgg tgctgttgga tactgcgcac gaggggtgtg gtaatactcc
    33781 caggcctgac gcagttcttc gttcggcgca tcggactcct tcattggtgc cagtggcata
    33841 atggcgtatt ctccgctgct aatatcgctg gttctggcgt ttgaacccgc ttcaacgtat
    33901 ggcagggcat caatagattt cacattgttt tcccaaccat tacggaacat cgaaccaata
    33961 ttgaccgcac taacggtacc gatggccttg atgcggcgat cctgaattgc agcattggct
    34021 gtatatcctg caccggcaca aattcccatc gcaccaattc gggtattgtc gacataagaa
    34081 agcgttgtca ggtaatcaat cacggcactg acgtcttcag tacgaatgta tgggttttct
    34141 aactgacgcg gctcgccgcc actttcacct tgataagatg cgtcataagc aatagtgaca
    34201 aatccctttt ccgccagttt ttcggcatag gtcccggccg tttgttcttt aacgccccca
    34261 cctgggtgag ataccacaat tgccggatat tgacgggttt catcaaattt tggaggaaaa
    34321 tagatcactg cagacaaaga gatggttgga ttattgctgt tagtgaagct gactttatta
    34381 ttcatcattc cgttcctcat ggagttgttg gttcgtttta acggttggtg atatcactat
    34441 agatattgat cattaagttg attagaccca aaatcatgat tagacctatc atttaaatga
    34501 ttaatagatt gtatggagat actgaatgaa gcgtgaagag atcgctgatc tgatggcgtt
    34561 tgtcgtcgtt gcagaggagc gtagcttcac tcgcgcagca gcccgcctga gcatggcgca
    34621 gtcagcttta agccagatag tgcgccgtat agaagaacga ttgggattgc ggcttttgac
    34681 gcgaaccacg cgcagtgttg tacccactga agcgggcgag catcttttgt ctgttcttgg
    34741 ccctatgctg catgacatag attctgccct ggcatccctg agcgatctgc agaaccgccc
    34801 atccgggaca atacgtatta ctactgtaga acatgcagca aaaacgatat tgttaccagc
    34861 catgcgcaca ttcctgaaat cacaccctga aattgatatt cagcttatca ttgattatgg
    34921 tttgactgat gtcgtttctg aacgttttga tgcaggcgtc cgtctgggtg gggagatgga
    34981 taaagatatg atcgccattc gaatcgggcc agatatacca atggctattg ttggctcacc
    35041 ggattatttt tctcgccgaa gtgttccaac gtcagtgtca caattaatag atcatcaggc
    35101 aattaatttg tatcttccca catcgggtac agcaaatcgc tggagattaa tacgcggtgg
    35161 acgtgaagtt cgtgttcgca tggaaggtca gcttttactg aatacgatag acctgatcat
    35221 tgatgctgca attgatgggc atggattggc gtatctacct tatgatcagg ttgagcgggc
    35281 tattaaagaa aaaaaactga tacgtgtttt ggataaattc acaccagatt tacccggtta
    35341 tcacctgtac tatccacacc gtcgacatgc tggctcggca ttctcattat ttatagatag
    35401 gctgaagtat aaaggtgctg tttagcacta cttgctgata cattaattta attcttctct
    35461 taacgtattc tcagttcctt tcaacgtttt ggttattttt tattcttcgt acaatggcga
    35521 cagatgctga ttatgatacc gaaaactggt ttgaacgtgc gaagcccgaa cgtagtgttc
    35581 ggtgttctat gtgctttgcc gcattttggc gtctattatt tacactaaat ctgatttaat
    35641 atattgatac ataaaacatt ttatgcttcc tgtgtcactt ttttgatgga agttgtttgc
    35701 atttctttaa ggcgaaacaa ataattacgc atcaatttta atgtcggtta gagggaaact
    35761 tatgaagcac tctgtttcag tcacgtgttg tgcgctgttg gtcagcagca tttctctttc
    35821 gtatgctgca gaagttccga gcggcacagt actggcagag aagcaggagc tggtgcgcca
    35881 cattaaagat gagcctgcgt cgctggatcc cgctaaagcc gtgggcctgc cagagattca
    35941 ggtcattcgc gatctgtttg aaggactggt gaatcagaac gagaaagggg agattgtccc
    36001 cggcgttgcg actcagtgga aaagtaatga caaccgtatc tggactttta ccctgcgcga
    36061 taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa gattttgtct acagctggca
    36121 gcgtctggtg gacccgaaaa cgctatcgcc atttgcatgg tttgccgcgc tggcgggaat
    36181 caacaacgca caggcgatta ttgatggtaa agctacgcct gaccagcttg gcgtcaccgc
    36241 agttgatgcc catactttga aaattcagct tgataaaccg ttgccgtggt ttgtgaattt
    36301 aaccgctaac tttgccttct tcccggtgca aaaagccaac gtagaaagcg gtaaagagtg
    36361 gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt cttaaagagc gcgtagtcaa
    36421 tgaaaaactg gtcgtggtac cgaataccaa ttattgggat aacgccaaaa cggtactgca
    36481 aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc actaagcgtt acctcgcggg
    36541 ggatattgat atcaccgaat ccttcccgaa aaatatgtat cagaagctgt tgaaggatat
    36601 tccggggcag gtttatacgc cgccgcagct cgggacctat tattatgcgt ttaacacgca
    36661 aaaagggccg acggcagatc agcgcgttcg tctggcatta agtatgacga tagatcgccg
    36721 cctgatgacc gaaaaagtat tagggacggg cgaaaagcca gcgtggcatt ttacaccaga
    36781 tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa caaatgagtc aggaagaact
    36841 gaatgcgcag gcaaaaactt tgttgagcgc agctggttat ggtccgcaaa aaccgctgaa
    36901 gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa attgcgattg ctgtagcatc
    36961 gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa aatcaggaat ggaaaaccta
    37021 tatcgatagc cgtaacaccg gcaattttga tgttatccgc gcctcatggg tgggggatta
    37081 taatgaaccc tccactttcc tgacattatt aacgtcaacg cattcaggaa atatttcacg
    37141 ctttaacaat ccggcatacg acaaagttct ggcccaggca tcgacggaaa ataccgttaa
    37201 agcgcgtaat gccgattaca acgcggcaga aaaaatcctc atggagcaag caccgattgc
    37261 accaatttat caatatacca atggacgatt aatcaaaccg tggctgaaag gttatcccat
    37321 taataaccct gaagatgtgg cgtacagtcg gactatgtat attgtgaagc attgatgtga
    37381 tgggaactgg cgttaccctt gcggataacg ccagtgatgt ctgatttagc gaccctgttc
    37441 cggcggtgta atgttatcca tatacagcgt ctggctgggg aaggcaaagt ccgcgccgtg
    37501 tgactgtaca atatcgataa tcttcaaata aacgtcttgc tgtgcagcaa gccactcagc
    37561 ccataccgtg gttttggtaa agcaataaac cataatattc aatgacgagt cagcaaactg
    37621 gttgaaataa accagtaagg tttgtcgctg gtcgatggcc gggtgatttt tcagcatctc
    37681 acgtacggct tcgacaataa cgcccacttt tgccgcatcc tcataacgta aaccaatggt
    37741 cgtggtaatg cggcggttgg tcattcgtcc tgggttttct acgctgatcg acgaaaacag
    37801 cgagttcggt acgtacaatg gacgattatc aaaggtcgta attttggtaa ttcgccagcc
    37861 aatttccgct actgtacctt cgatatttct gtccggtgaa cggatccagt cgccaatact
    37921 gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa ttactcagaa tatctttacc
    37981 ggccataccg acagccagac caccaatacc accaaaggtc agcaagccag aaaggctcat
    38041 gccgaaatgt tcgccataaa gcagaacaag caccacaata atggtgattt tgataatgcg
    38101 cgacataatc cgcgcactgg tgatatcgcg acctttttta atctgctgtt tttcaaactg
    38161 attaatcagc aaaaatagct taatcgtcag aataaccgca atcagggacg tacagataaa
    38221 atcgataacg cctggggtga taaatttgag tttatagttt tctataacat aattaataat
    38281 gctaccaaca gcactgataa ttatggtgta gattaaaaat tgcaccgcat ggaataaaaa
    38341 accttttctt ttacgatttc cgcggcgaaa ccaaaagctc atcagaatca atgctgcgca
    38401 gctaccgaaa ataatgacca gattaagcgc attatttgta aacagttcag cgatcattgt
    38461 tttatcaggc tcctccagat aattgtcgtc atgccggaaa cccctggcgg ggctatttta
    38521 ccgcgacaat tcattcagat catcaatagt cagggaagga agtagcaaca ttagctaact
    38581 aatcaatgcg ttgattgtaa atccagctaa aaggtgaggt tttcagagca gacaacggtg
    38641 aaatgtcatg gtattgttac gtttaggtaa caagaaattt gtctgcacaa ggattacatc
    38701 atgattatgg cgaaactgaa gtcagcgaaa gggaagaaat ttctctttgg tttgttggcg
    38761 gttttcatta ttgcggcgtc ggttgtgact cgcgcgacta tcggcggcgt tatagaacag
    38821 tacaatattc cgctgtctga gtggacgaca tcaatgtatg tgattcagtc atcgatgatt
    38881 tttgtttata gcctggtctt tactgtgttg ctggcaatcc cgttgggaat ttatttcctt
    38941 ggcggcgaag agcagtaagt aaaaaatagg cccgataact cgggccttgt cagttattga
    39001 agagtcgtta atcgtcttct tcgtcatcca gttcaacggg tgtctgatac tggtcaggtt
    39061 taatgactaa cagatcgcag cgaagatgat caatcacctg ttccgccgtg ttgccgagga
    39121 atgctgctga aataccggtg cgtcctaccg tgcccagaac cacaatcccc gcctgtaagt
    39181 gctccgccaa atcaggaatc acctcttctg gcagaccttt ttctacgtgc gtcatgtttt
    39241 cattaatgcc gaatttctgc cgcagagctt tcattgccag caaatgttgc ccacgaatgg
    39301 catcgttata aacgctcgga tcaaattccg gcagttcaat cgcgatatta attggcgtta
    39361 ctgggtaagc gccaaccaga tgaacttcgg tatggttgac ttgttctgcc agttcgatcg
    39421 tctctttgac cagtttttca ttgagcgcat tatgatacgg ctcttcactg gcgagattca
    39481 ccgccaccag cgccttacct ccttccggcc acggctggtc tttcaccatc cacaccgggc
    39541 ttgggcattt gcgtaacaga tgccagtccg ttggcgtaaa aatcaccgct tccagacggt
    39601 catgttggtg cgccattttt agcaccaaat cgtgcccgcc gctgatcact tcctgaatga
    39661 tggcttcgaa aggacggtta tgccagacca ctttaatttc aatgggaacg ccagcattga
    39721 gataatattt tgcctgctcg tggatccagg ctgtacgctg gctgatgacg ccctgacgca
    39781 tagcggtacg ttcgtccggg gagagcaggg tggtcatttc gtatgagaag tcatagatcg
    39841 gcaaaaaggc tttaattttg ccaccaatcc gttgatgtaa ataaacagct cgccgcaatg
    39901 ctggttggtc gtcctggtta ggatcgataa caacgagcat gttctgatac atagccatac
    39961 agggtctcct tacaacaact gtcaacgcag tttgtaatta aaagattaac ccatatctgg
    40021 tgaatgaaac agtgatgaac cttctgccag atcaataaat cagaaaaatt taatgatatg
    40081 acagaaggat agtgagttgt gcggaaaaat caggcaacgt tacgcgtatg accagcaagc
    40141 tgggccagcg catcgttatt ttcgatggtg atgtatttac ctttgactgc cagcataccg
    40201 cttttctgga agcgacccag cagacggctg atggtttcta ccgtcaggcc cagatagtta
    40261 ccgatatcgc cacgagtcat cgtcaggcgg aattcacgag gggagaagcc gcgttgggca
    40321 aaacgacggg acaggttgta gatgaatgca gccagacgtt cctcggcatt tttcttcgac
    40381 aacagcagga tcatgtcctg atcgcctttg atttcaccgc tcatcagacg catcatctgc
    40441 tgacgcagat tcggcatttt accggacaaa tcgtccagcg tttcgaacgg gatttcacat
    40501 accatcgagg tttccagcgc ctgcgcgaag ctcgggtgat ggccgctgcc gatggcgtca
    40561 aatcccacca ggtcgcctgc taaatggaaa ccagtgattt gctcgtcgcc ttgctcagtg
    40621 atggtataac ttttaatcgt accggagcgg atggcataaa gcgatttaag ttcatcacca
    40681 gccttaaaca gcgtctggcc tttctgaata ggcttcttcc gctcaatgat attatcaagc
    40741 tgatcaagct catgttcgtt gagtgtgaac gggatgcaaa gctggctgat gctgcaatcc
    40801 tggcaatgga tagcacaacc gccagactga atgcgccgta taattcgctt ttccgggatc
    40861 ataggtctgc tcaagccgta attgatattt gtcaatttta acatcttttt agggagcaag
    40921 taagtctaag caaaccttaa cagcagagaa ttccgatatt agatgtaaat atatgtctat
    40981 ctatttgaaa acccttaagt tgttaagggt aactttacat aaaagtgtga acaagctggc
    41041 acaaattgtt taatgtttac agcaaaagat aaccttcatg gcgcaataac cactcttttc
    41101 gctgaactcc gcctgcatat ccggtcatgg tgccgtttcg gccaataacc cgatggcaag
    41161 gtacgacgat gctgatggga ttcgatccgt ttgccgcacc aacggcacgc gccgcgccag
    41221 ggcggcccaa ttgttcagcc agttggccgt aatgcattac ctgcccgcag gggatagtgc
    41281 gtagtgtttt ccagacttcg cgctgaaatg gcgtcccccc tgtggcagtg ggaagcgtat
    41341 caataatgct aagattaccg gcaaaataat cacgaagctt gtcgcttaaa ccgcctgggt
    41401 tggtggcaga aatgcgctca tagccttctt tgcgataatg gatgtccagc agctgcacca
    41461 tgcgttcgct gtactcttcc cattcaaccg cccgcaggcg aaattgctca tcgcaaatca
    41521 cccacagtgg acccagtggc gtggcaattt tttcttcaag taatctcagc atccgttctc
    41581 tcttaagaca aacgtgggta aatacccgga cctatcggca gaccgacaag ataccacgcc
    41641 agcaacatca gcagccatac caccaaaaag ataagtggat agggcaagac taacgaatag
    41701 taagtaccca gtttcgcgtc tggtttgtag cgttgcagga atccaagaaa cagtggaaca
    41761 aaaggagata ccggcgctaa aggcaatacg gatgagtcgg caatacgaaa gaggatttgc
    41821 gcaaatgccg ggtgaaagcc aagtagcata aacattggta cgaaaatggg ggccagaatc
    41881 gaccagattg cggaaccgct ggcaataaac atgcataaga aagaggaaag caacgccaga
    41941 ccgacaaacg ccgggatgcc gctaagccct gaactttcca gtatatcggt cagccccacg
    42001 gcgatgaatt tccccatgtt gctccagtta aacatggcga caaactgggc gaggggaaaa
    42061 accatcacga taaatcccgc catctctttc atcggttcaa tcattaaatg cggtaaatcc
    42121 gcctgacgtc gaattgtgcg ggtagcgatg ccataagcca gcgagacaac aaagaaaaaa
    42181 agaatgatca gtggcacgat tcctttaata aagggtgatg gcatcacggt gtgattaatc
    42241 ggatcgcgca atatcccgtt ttccgggatc accatcagcg caatcgcagc aataaaaagt
    42301 agcgatacga cacctgctat gcgtaaacca aaacgctgac tttcggtcaa tgtctgcagt
    42361 ttctcatcgc tgtttccctg ccattgacct aaccgtggct cgatgatttt gtcggttatc
    42421 aggccgccaa caatcgtcag tacgactacg gagctggcca taaaatacca gttatcaatt
    42481 acactgacgt gcatttgcgg attgaacgca gccgccgctt ccgtgctgat ccccgacagc
    42541 aacacgtcgg ttgtgacaat cagtaaatta gccgtaaagc cgcaacctac gcctgcaatg
    42601 gcagccagta aacctgcaac tggatgcctg cccaccgcca gaaaaatcag cgcacccatc
    42661 ggtggcatga tcactaacgc cgcatcggaa gaaatgtggc tgaaaaaagc aataaacagc
    42721 accatataac tggcgtagcg ggcattaaca tgcgatgcca ttttaaccat tagtgctggc
    42781 agtaagccga cgcgctccgc cagaccggca cctaaaacca gcgctaggat cgcaccaagt
    42841 ggagcaaaac cgctaaagtt tttaataaca ttgggtaaaa accagtgtaa tccttcaaca
    42901 ctgagcaggt ttttcaccac caccggcgta ccatcggtcg ggtttttcgc actgacgcca
    42961 aaggccgaca aaattgccgt cgtcaccatg agtacgataa tcaaatagat aaagagcaaa
    43021 aaaggatggg gaaccttgtt accaattctt tcgacccagc catagagctt cccggattgg
    43081 gaggacgacg gtatggatga catactcatg ggcattcctc ggttgttgtg tttgcgttgt
    43141 tgttgttatt ttaaaggtga cggtgtcacg tttttcggga tagggcagtg gtacggttgc
    43201 gtgtccgtta cttgctgatg ctcttgttgg cattcttgca atagccctga atcaaggaag
    43261 agattgactg tggttgctgc catagttttc gccgccagca gcattccttt atgagcaata
    43321 gatgttcgcc cctggctaac cagttgccac gtatgtaggg gtgtaccgac ggcaaaacag
    43381 gggctgaaac actgggcaac aggcagtttc caactgacgt cgccgacatc agtcgatgcc
    43441 gcaagcacgt tatcggtggc ggcatatgga gcgacttcat tcgccagtac cgtttcacga
    43501 tgacgtagtg caaaaacctt gccgttttcg ccaccggttg cggcgatatt attcagactg
    43561 ttttgccgat cgttggaggt gagcgtagcc tgaatttgtt tcgcaaaagc cagttcttcg
    43621 gagttccatt ccggggtacc aaaatgggat agggcctggt acatggcatt ttctaaggtg
    43681 cgattcggga gataactgga acaggctttg tcgaagcggc attcaacctt ggtttcggtc
    43741 atcaatgccg caccttcggc gattttggcg acccgatcat aaatatgctg cacgtcggtc
    43801 atttcggggg cgcggataag ataaagcact tctgcctgcg cctggaccac gttgggcgag
    43861 atcccgccgc tatttgtgat ggcatagtgt acgcgcgctt tttcaataat atgttcgttg
    43921 aggaagttgg tgccagtggt catcaacgtt acggcatcaa gggcgctgcg tcccaaatga
    43981 ggggaattcg cggcatgtgc tgcgatccct ttaaagcgcc atgatgcctg aatgtttgcc
    44041 agcgtgcggg tattgaacat accggcaaag gcttccgggt gccaggtgag tgccgcatcc
    44101 acatcatcaa ataccccctc gcgaaccatg aacgttttac ccgagccgcc ttcttcgcca
    44161 ggacaaccat aaaagcgcac cgtgccgcct tgcccatatt gttccagcca tttcttgacg
    44221 gctattgcag cggcaaaggc ggcggttccc agcaaattgt gtccgcaacc gtgaccattt
    44281 tcaccgggcg tcacggatgt aggttgcgcg caacctgctt gctgacttaa acctgccagg
    44341 gcgtcatatt ctcccagcag ggcgataacc ggtttgcctt gaccaaacga agcaataaag
    44401 gcatttggga tattgcctac gttgcgggta acggtgaagc ctgcagattc cagcgccgaa
    44461 gccagatgct ccgctgacca gaactcttca aaacgtgttt ctggatgatc ccagatttga
    44521 tcggcaatat cggtataacg ttggcgatcg gcttcaatcg catcgtcgat aaaacgatag
    44581 atttcctgca tcagatacct cgcgtccagg gaaaattgag cgcggtgcgc gccagcgttt
    44641 cgacggcaat agcgagaacc tgctcgtcaa aatcgaattt ttcgttgtga tgacctgccg
    44701 ccagctgtgt gccaaacacc acgtaggagg cttgcccttg atgttgctgc acgcgggcca
    44761 tcattaatgt ggcatcttcg gaacccgcag gcgcttcaac acgttcaatg gcctgattaa
    44821 cccccgctac ctgagccgcc tgactttgca accatgcgac ccattgcggc gaaggagaac
    44881 tggcggtagc tgcacccatc agacgagttt caacgccgac accatacatg gttgctgcgc
    44941 cctgaatcgc ttgttgtgca cggtcaaaaa catattgatt aatgacgtcg ctggccccgc
    45001 gtgtttccac tttcagcaac gccgaggcag gaacaacatt acgaccgctt cctgcctgca
    45061 taacgcccac gtttactctg gaagcgcctt cgctgtgcgg ggcgattgca tgtagagcaa
    45121 gagttgcttg tgctgccgcc aacaaggcat tgtgaccgtc ttctggtttt gcgcctgcgt
    45181 gagcggcggt cccggtgaag tgcgcgtcaa atttggtggt tgccataaaa ttatcactgc
    45241 cgcacaccac ggtgcccgca ggtacgccag tgccaatgtg cacggcagta aaataatcaa
    45301 catcatctac gacacctgca tcgaccatcg cccgcgcgcc acgcgtacct tcttctgcag
    45361 gttgaaaaat cagtttgatg atgccatgta gtccggactc gaactgttta agggtatgcg
    45421 ccagcccaag cccaatggcg gtatgcccat catgaccaca ggcatgcatc attccggcgt
    45481 tacatgacgc aaaaccgtcg cggtaggggc gatggctgac atcctgctct tcactgagat
    45541 ccagcgcgtc catatcgaca cggaaagcca tcaccggacc ggggcgaccg gtatccaggg
    45601 tggcgacgat acccgtgaaa ccaccttcaa aagccgcaat ccattgtgct agcgcaccct
    45661 gttgacgagc gcgctcgaat tcgcgttgta gagtgagttc atcaggtagt cccatccgac
    45721 tactttcatt aactacttcg cggcccagcg ccagtgaata gccgagctgc tgcaattctt
    45781 ccgcaacaag ggtggcagtg cggaattcca cccagccaga ctctgcatag tgatgaaaat
    45841 cacgtcgcca gtgcgataat tttggggcaa gcgaattaac aaattgattc aaagactcca
    45901 taacctttcc cgtcatcagt aaaaagtgtg acccggttca cgtagcgata gtttttactt
    45961 atcactaact gatttttcac agttttaacc gttcataaat taccctgaca caatcatctg
    46021 cattaaagta gatgccagtt tctttggtct gataaataac ggttatcggt ggcgtcatgg
    46081 cttttcaggt aaaaattcat caaattcggg cttttgttga agtggctcgt cagggcagca
    46141 ttcgcggagc gagccgaatg ttgaatatgt cgcaaccggc actgagtaaa tctattcagg
    46201 agctggaaga agggttagcg gcgcaactct tttttcgccg tagtaaaggt gtgacgttaa
    46261 ctgatgccgg tgaaagtttt tatcagcacg ccagtcttat tcttgaagag ctgcgcgcag
    46321 cccaagagga tattcgccaa cgacaagggc aactggcagg gcagattaat atcggcatgg
    46381 gggccagtat ttcccgcagt ctgatgccag ctgtcatatc tcgttttcat cagcagcatc
    46441 cgcaggtaaa agtacgcatt atggaagggc aactggtgtc gatgattaat gaattgcgtc
    46501 agggagaatt ggatttcacc atcaatacct attatcaggg accgtacgac cacgaattta
    46561 cttttgagaa attactggaa aagcaattcg cgatcttttg ccgcccggga caccccgcca
    46621 ttggtgcccg ttcgatcaaa cagttactgg attacagctg gacaatgccg acgccacacg
    46681 gcagctacta caaacagttg agtgaattgc ttgacgatca ggcgcaaacg ccacaggtcg
    46741 gtgtagtctg cgagacgttc tcagcctgta tcagtctggt ggcaaaaagc gattttctca
    46801 gcatactgcc tgaagaaatg ggctgcgatc ccttgcacgg acaggggctg gtgatgttgc
    46861 cggttagcga aattttaccg aaagcggcct attatttgat tcagcggcgt gatagtcgcc
    46921 agacaccact gaccgcgtca ttaatcacgc aattccggcg agaatgcggc tatctgcaaa
    46981 gttaaaactg cataaaaaaa tagagtctgt cgacatccgc cagactctac agtacacaca
    47041 gcagtgcatc cgcgtcttaa atccggcatt gtctcctctg cgccggtgac tgtgttataa
    47101 cggttttaac ctttagttgc caattttctt cagtgacaga tttcacgaaa atataagtta
    47161 cattattaat atcgtgaatg aataatcatg cataagcatt ttgcttaaaa tatcggcaat
    47221 atttggaact tattactgga attttgggta atacgttgtt ggaccgaccc ggtctggtta
    47281 tcatatcgcg ctcttaattg cgggaggatg taacatgaac cttgacgaca aatcgctgtt
    47341 tcttgacgcc atggaagatg tccagccgct gaaacgtgct accgatgtcc actggcatcc
    47401 aacgcgtaac caacgtgcgc cgcagcgtat cgacacgctg cagcttgata atttcctcac
    47461 taccggattt ctcgacatta tcccgctaag tcagccgctg gagtttcggc gggaagggtt
    47521 gcaacatggg gtgctggata agctgcgtag tggtaaatat ccgcaacagg cgagcctgaa
    47581 tcttttgcgc cagccggtgg aagagtgccg caaaatggtg ttcagtttca ttcaacaagc
    47641 cctggcggat ggtttgcgta acgtgctgat tattcatggt aaagggcggg atgataaatc
    47701 gcatgccaat attgtccgca gctatgtggc gcgctggctg accgaatttg atgatgttca
    47761 ggcatattgc accgcgctac cgcatcatgg cggcagcggg gcgtgttacg tcgcactacg
    47821 taaaacggcg caggcgaagc aagaaaactg ggagcgccac gctaagcgca gtcgttgatc
    47881 tcgagacgca tccgcggctt atgccgccag cacgcggttg cgtccatcat ttttcgcccg
    47941 atacaaagca tcatcaacgc gtttaaacag ttcatcgatg ctttcatttc cttcgtgatg
    48001 cgccacacca atgctgacgg taaagcgtgg taagcccgaa atactcactt ttgccacgct
    48061 tacgcggata gtttcagcca gcgaaagtgc agtatccagt ggggttcttg gtagcaataa
    48121 gacaaactct tcgcctcccc aacgaaacac caaatcgcct ttgcgagcgc aactttcgag
    48181 ggtgcgggcg agggcgcata acacctcatc acctttagaa tgcccataga gatcgttaat
    48241 gtgtttaaaa cgatcggtgt cgatgagcaa caagctgtaa tcctgagcga tggcgagatg
    48301 ctgcatttgg cctggttccg taatgtgata aaactgtcgc cgattcagta atccggtcat
    48361 cgcgtcatgg tgagcagcat gttccagctg ctcctccagc cgtttttgct cagtaatatc
    48421 atgcacaata cataacatga gcttgtcgcc ataaatttca atcggtccgg cataggtctg
    48481 cacatgacga gtagatccat ccgccagttt atgaacaaaa ttcaaaggtt tatgaccgcc
    48541 gggtaaatgc gaaatttcat gcatgatagg catgacgcga cgcccgagca tatttatttc
    48601 ccaggtatgt ttctggcaca tcgtttcatg gttataacca tagaaattga gcgcggcgag
    48661 gttagcatcg acgatttgtc catctcgtga cgggtcaatc aacaacattg gtgcagagtt
    48721 agtcagaaaa aagcgcgcat aaaaaccttg ttttttgcgc tgataatttg ccgagcgact
    48781 ggcttttaaa cccagcgttg ccggcgcttc gataccttcg aaaataatca ccggttctgt
    48841 ttctgtcagc tctcgcaaaa caagccgaca gcttaatgct gtttcctctt ctttacgctg
    48901 aacagtgagg atttcgataa tatcgtgttg gttttgcaga tcggagaggt atttcggcag
    48961 ttctttttgt gaggagacgg aatagggacc ggttcgtagc tgactaaacg tgaggtcttg
    49021 catcaacagt cgcgccgcgc tattggcata aattaactgt tcctcaaagg gcgaaacgat
    49081 ccagacagta acgattcaca gtgtttagtt gcgttcagaa ttgttaatgg atgataaaaa
    49141 atatcattta aattcactaa gttacatatc aatactcagc agatgtgttt taatccgttc
    49201 gatgatgtta atatccgttc agataaaatt gtgtaccaaa tcgtgaccaa aaaagaggtg
    49261 tcataatgct gacggatacg aaattacgcg caatagcaaa caagccgtat gacggcccga
    49321 acgagatagc agatcgcgat ggcttgtctg ccaggataag cccgaagggg aaaattattt
    49381 tccagttcag gtacaggttc aacggtaaac ctgtaaggat gaagatcggc gactacggca
    49441 gcatgacgtt aaaagatgca cgctacgcgg tggcggaata caaagaacta ttagcgaagg
    49501 ggaagaaccc aactgtttca gtaaagcaag cgataagtat tgagcgtggc aaattaacga
    49561 taagcgccat tgtgagcgac tatttatcca tgccgaacgt tattaagctg gttgcatata
    49621 atgacgtcag gagcgcactt aagcgtcatg ttgtagataa attcggtgag tatattgccg
    49681 atgatgtcag tacgaaacaa tggatggata tattcggcga gatcactgcg gcaggccacg
    49741 cggttacggc tggcctaata cttaagcgaa tgaagattgt tgtgaacagt gctatccggc
    49801 gcgggctaat gaccagtacg gcgataaaca acatcagggt tattgacgtt ggcgaaaatt
    49861 atgcggttgg cgaacgttat ttatcatgcg atgaaatagg ccatttctgg cgaacggtgg
    49921 atgattcgca gatctaccgt cagaacaaga tagcattaaa gctattgttg ctcaccgggt
    49981 gcagggtggg agaattgcag aaaatgcgga gggaacacct cgacctggat aacggtgtgt
    50041 ggaccgttcc ggcagcggtg gcgaagacaa ggagtgaaat caggcgtgga ttatctgatc
    50101 tgtcaataga aatgctcagg gaggtattag cctcccatga gtatgattat gtgttcccgc
    50161 cagtgattaa cggcggtaac aggccagtaa acgcatcagt gataaaagta gctgcaatct
    50221 tcgtgcgtaa aagaatgggc ggtaagtcgt ggtcctgtca cgacctccgc cggacgtgca
    50281 ggacgcacct gtcagccata ggtgttccgg tacatatcgc agaaaagatt ttgggccatt
    50341 cactcagggg aatactggcg gtgtacgaca aatatgatta tcttgatgaa caaaaggagg
    50401 cgctaaataa gtttgcggat tatgtattat ctttggctga cgtcaaacca atctcatcaa
    50461 agaaccgcat tacatcctga tagcgataca tgacgccccg ccccgttccc agcacatcaa
    50521 ctgggtcagg gaatggggtt ccgttttcct cccacttttt acgccatgaa taaaacgttc
    50581 ccctgctaat tccgcccagc atacggcaga tggcagggcg actaagaaga attgttgccg
    50641 tcttatccat tatttattcc ccgtgtattc aacgtgtgcg atacatccat caataattgc
    50701 ttgcgccatg ttgcgataat cgcacgaaaa atcattgtcg tggttaaagt caatctcttt
    50761 tgcagcgtct tcccccataa cattaacagc aagcgaaaac gcgatttcag caagtgcctt
    50821 ttctttcgga tctacataca acttatgagc gaaatgcttc cattccccgg cttgttcaaa
    50881 atcaggcgca tccatttcta caaaaagagc aaggcgataa cttatatact tgtatttcag
    50941 aataacatat ttacgaccat cgacaggatc tagaagctca agccatactc cgcatggagg
    51001 cttttcgcct acggcccatt ccgttttttc tggttgtgtt tcgcaatcat gccgctcatc
    51061 ttcgattaat tcatcaatga tttcactttc tttattcatg ccgtcatcat ccttattcga
    51121 taaattagcg tatttatcaa atttccagct tgcagccata accgaataag tgccgccagt
    51181 gtcagcgcga acacccaata aatagtatga tccaaaatta tattctgtgc ggcatgttgt
    51241 tacctcaaac gatgggtaac actcagggtt agtgcgatgg ctaatcacgc caattgcttt
    51301 aagtttattc atttgcttat cctcaattac gccaccgtta cattgtcaac tttgatgaaa
    51361 tattcaggat ggttttctat ttcttgcttg aagtgcgcgt gacacaatcg ccattcacgc
    51421 gcatggttgt gaaagtaata aacgtattcg ccatcaacct tatacaaaat acctgtaggt
    51481 ttagccatgt agtaacgaac cattatcgcc cccagcgttt tataaactcc tcgtttaact
    51541 tcgtatcgcc agaccactga acaccatgtt ccgcgccgaa cgagtagatc agttcgatta
    51601 gctcgctaaa ttctgcttta ctcattcggc tggttgacgt acccagcaca acaaaacctg
    51661 atttatccag gttagggaca acgccatatt tcttaagccc ggcagtaaat accgctttcc
    51721 agtcttccgg cgacagcttt ttgccgtacc aattaacctg atcgctaacg tctgtaagca
    51781 gcgcccaaag caacgcgttt tgactcagcg agcgtgtttt ctcttggatg gtaattatca
    51841 gcgggctttt gctatcgggc tggatctctc ttatctgcct gatagcgttt tcttttacgg
    51901 cgtcgttgac tatttcaaat cttatttgcc tcatgacaca ccgttactat ttttccaccg
    51961 tgcccgcgca tacatgatcg cccgcaaaga aattagtcgc gagcttgtca tgaaagcgct
    52021 ttttaaatat ctcatatcaa caacaacagg cttatcagga tcgtcgccgc gcatgtattc
    52081 cgcaattgtg cttaagtctt ccaccgtcaa atctaattcc ctattcatga tcgccacctt
    52141 acacaatacg cattgtgttt cgtaattcac cgcgcaacgc ctgcaacgcg taatgaatag
    52201 gcacaaaagc ccggttaaat tttacgtgtc tgtatttgcg cattagtggc ggcgtataaa
    52261 ccatcgtgcc gcaaggatcg catgtgaatt taaagtgaga ttcgtcaata aacgtttcaa
    52321 ctgaataaaa aattacccgt ctcataattc caaccccgtt tcgccgttaa gtttttcaat
    52381 ctcaaatatt ggtttatccg gtagcaggcc gttatttttc cggtattcgt tcaggcgtct
    52441 gtcaaagtca aacgctaaat cattagcgtg accgaatcgc cctggtttaa acagcgagta
    52501 aaaattaacc ccgtgcctgt ttggatcaac gcaaagcgtt tcatcaatca ccattaaacg
    52561 cagagtacga gaaaggtaat tccacggtat tccagtttcc gcgctaatgt cgcgcatacg
    52621 ctttttaacg ctgtagtcac caaataacga cgcgacgcgc tcacgacgtt cagcatataa
    52681 ctgtttaatg cgataaccca gcagtcggcg gcggcgtcca aagcatgtat acacgcgctc
    52741 gacgatgccg ttttttatta agtgtttaat tgtctcaggc atgattccat ccttatcata
    52801 tccagccaat aaccccgttt tattgcgtaa ctggtgcatt gttgcgatgc catcaagttc
    52861 aaggatattg ataacttttg tttcaagttc agcgctcatt gtttttagcc tcatttaaaa
    52921 cgcgacaaca accaccagaa agcggtaaac tttccggtaa gtcgtttgct cagattcatt
    52981 aaaaagatgt gttattctga ttgtttcgtt ggttaaaacg gcttgtgcga tgattcattg
    53041 actgaataca cgcggcggct gcgcgagcct ggtcgcatgg caagatatta ccgttgtcat
    53101 caaaccgctg gtaaacagtt ccagacttac catgacggtt ttttgacaca atgatttcca
    53161 tgtattcgcg ggcaacagac tgttcgtcgt aatacccatc gcggtaaacc atgattattc
    53221 tgtcagcgtc ttgttcaagg ttgccggaat cacgaagatc agaattatta gggcgcttgt
    53281 ttggtcgttc ctcaactctg cgtgataatt gcgccagtgc caacactggt acgcgcaatt
    53341 ctttcgccat catctttaac gaccacgata attgccctac ggcgagatcg tgccgttcgg
    53401 ctttagccag cttcatcaac ccgatatagt caatcattac catacctagg tttggatgtt
    53461 cctgtttcat tcgttccgtg gtggctcgta tttcctcaac cgttaactgc gacgcgtcaa
    53521 cgatccacac atcaagatta gcaagtgcgc tcataccctg gccgacatgc gcccacccct
    53581 catcatcaag ttttaccgga ttgcgcaggc agtcagttga taaattacca gcgccagcga
    53641 tagcgcgttc agtcatctga tcgagtgaca tttcaagcgt gaagaacaaa acgccaaccc
    53701 gctgatcctt gctgccaggg taagggcgtt cagcagcagc gcgggcaatt gccagcgcca
    53761 acgcagattt accagaccca gggcgtccgg cgagaagcac caaatcagta gcgttaatgc
    53821 cgcctaacat ttcgtcgagt ggttctatcc ctgttttgat gttgtcagaa ttgacaccgc
    53881 attccatgcg cttacttaaa acttctgtgt actcctggac agcatcgcgc aacagaacag
    53941 ggataatctt gtctttcgtt acctttaatt ttgaataccg ggaatcaaaa tctttcatcg
    54001 tctctttgac gacctcaagc gtccccgtct ccagtttgta gcgcatatcg tcgatgagtt
    54061 ccaacatctg ccgccgctgg tattcttcac gtagtagctg tgcgtatccc ttcaggttgg
    54121 cagcagacgg gcacgatctc gccgtttgca ttagcggtac gaaattttca ttcccaatct
    54181 catcacctac cagcaatgca tctatgagat tcctgtttct ggcttgcgcc ctgataattt
    54241 caaacgcacg tttatacagc ggaatagtga acacttcagg atcaagcgta gcgagaacat
    54301 cttgcgcgtt aggagtaagg ccgcccaaca gcagcccacc gataactgac gcctcacgtt
    54361 cctgccgtag tgagtttatt tgctcagtta ccattatgtc attacctcct gtttacaaaa
    54421 tgtattttcg attggtaaag aaattccgtg attactatga aagttatata ttctttctgc
    54481 ttcttttctt gctttcacgg catcatcaaa cgaatcaccg taataaacgt atattgtttt
    54541 ctttttaccg tcagatcccc ttatacctat ttgcgcccgc catttatggg ttttcttgtt
    54601 ccatgtaacg ccaacgcatc cactagtgtt atttgctggc attattttat ttctgttatt
    54661 ttcctgcctt gttaccattc ttaaattttc gattctatcg tcaccaggga tatggtttat
    54721 gtggtctatt tccattccat caggaatcgg tccgttaaac atttcccaaa taatgcggtg
    54781 acgccatgta caacagatac cattaacgca gatagtccaa tagccgcgat gatgcttact
    54841 tccagccacg ctacctgctt ttattttgtt atttgcgcgg ttacaagacc aatatagaac
    54901 gccgtcccta taggtaaata aatcatccca catcatgaca ccccaccatt tcaggtctat
    54961 atacaaagcg accaatttcg ccataatcag gactgtaaat gataaccgcc gcgcaacgtt
    55021 gcgatctcca gcctccatta gttgaataag cgtcttttcc cgctaacgtg ccgtggtgtt
    55081 caactattcc tatagatgat tctgttactg atttgtggtg caaatgtccg acatggacgt
    55141 aaacatattt cgacttgcca aagtcttccc gccagtcagc tacgcaagca gacaataaat
    55201 tttccggctt tttgatagtg tgcccgtggt ggtaagcaag gaatgttttc ccgtactgcg
    55261 tgtggtggac gatagtaggt gacacatcaa ccgtcacgcg aggttcatct tcgtaaaaag
    55321 ccgccagcgc cgcacgtagc cagatcattc ctgactggtc gtgattgccg gataatactt
    55381 ggatctctac gtccttatgg tttaataaca tcttacctac agcacgacgt acagaccgga
    55441 tcgccacata aacaagtttt gcgtagcgtg aatcgcaatc caagacgtga ttgcttgatg
    55501 gtgtaactgg taatagtccg tcagtgtgaa gcacatcacc gcctaacaac aaaacagcct
    55561 tttcagacat cggcgcagcg ccaaccgcgt agtcaaagaa gtcattcaat acgcgctcag
    55621 cgatcccagt gtcgtagctc tcgccgcatt cagctttgtg ggcaagtgcc ccgatatgca
    55681 aatcaaatac cggataaagg gccaggcttt tttgaaaatc aatttcaggc actggcacgg
    55741 cctccgcgcg tggtatttcc tccgtgaatg cgtcgcgggc agcttccatt agcctttcca
    55801 tttcatcgcg gtcacgggca gttttaatcc agcgcatgat cacattgccg ttcttatcga
    55861 ctagtaacga ctcgccattg acgccaaaac caggcgcgcg gcgcgttgat aataaaccgc
    55921 gtttcgccag ctttgcgccc aggcgttcga cgttacgttt tgacatgcca tatttttcag
    55981 cgatctgttt gtacgttaaa ccgttgttat attcagctat tagctgttca tcgctgatct
    56041 ttctttgcga cataatgtta cttcctctgg tttacatttt gtatattcat tgacagcaaa
    56101 taatctcttt atatgcttct tctaccatgt cgaatacttc cggcctgtaa taaaaaaatt
    56161 cttcgctacc gctaaatttt acaggaaagt taattttata attgctaaaa gccttatgca
    56221 tcgttctttc taattcatac atattttcgg ttgttccata tagagttttc acgatatgca
    56281 aatcagtaat tataacgcct gctttatgtg cgcttcttaa tacctgattt cttcgctcgt
    56341 tctccttaac acttacacct attttcattt gtgtaggtac ttccagatca tcaaccatga
    56401 tgtaaagttt tccgtgatcg tgggataaaa aaccgcgttt accgcatttc gggcaaccgt
    56461 gcccgcattt aaggtgacta ggctttgcta accacttgtg attacaaact ttacagcgac
    56521 acaatacctt cttcgcatct ccggtaattt tccctaaaac ttcaacatca tgattagcct
    56581 taacaatatt tgcaacttgt tctttgtgtg ttaatgttgc ttttaatctc gcgcatttcg
    56641 ggcaaccgtg cccgcattta agggcattag gtatagacga ccattcatgt ctacaaacct
    56701 tgcatcgaca taacactttc gttttgtctc ctgttatttc tcctgtaact tcaacatcag
    56761 gattaacctt agcaatggct acaacctgtt cttcgtgtgt taacttcgca tttccagcgc
    56821 attttggaca accctgacct cgcttgaggt tgcaaggtgt aggcgaccat tcatgtctac
    56881 aaaccttgca tctacatgtg acttttgttt tattgccagt aatttccccg atcacttcaa
    56941 catcaggatt gacgtcagca ataactgcaa cctgttcttc gtgtgttaac tttctcatcg
    57001 atccaactcc acaaaacgca ccagacgcta tacaacgcaa aattccaccg cgtaacccac
    57061 ttgtaagggt gcagtgtctt tttcgcgcca gagacgctat caggtggcta attttcgatt
    57121 tttaaggtga cagggtagtt atggattaac tagcagatac aaaccgccga acaaagcgac
    57181 attaacagcg atagccatcg cgatcgcgaa agccagggcg aacatgcgtt taccgttcat
    57241 agctaaatcc tctctcaatc gctctctaac gagctaatcg caaaaaggta agaagtgtag
    57301 caccccatgc gatttagccc gctggtgagc ttgttttgtg ggtaattttg gttctttagt
    57361 gatgatggat agtcaaagag cgccagcgcg tgtatcacgt agcgtttttg gtttaagcag
    57421 gaaatcaagt gtcgcagtaa agccattgcc gaagtaaaaa tctgaagccg tgtttttgaa
    57481 cgcctcaaag taagcaataa agccagtgat gcttttgtct ttcaggtagt cagtgaacgc
    57541 atagatcttg cgttccaaat cccgatccag ttcggcaggt ggtaacaggc cgtcgaatgt
    57601 ctggttaaat gctgatacca cgacagcagc gttaacagta gctgtaactc ttcgccattg
    57661 ttcagcatca gcaagatacc catcaaactt agttacccgg caaatgttga taggcttagg
    57721 tgttccacca cggttacgcc attgagttaa agcccactca atcactaatg tgatctcatc
    57781 ctccgtatac gctttacgtg tttttgtttc tgtcagtagt tcgatgaatg gttttgcatc
    57841 acgatatttg catcctgcct tgtcgttgta gaagtcaagg caacgcaatg ccgcttcgtt
    57901 tacaccatcc tgattgacat gtttttcttc ttgggggata caagggggta tatctttaat
    57961 gttctcttta ctggttatat atgactgatt agggttgctc tctgccacta cccccggttg
    58021 ctttgtgcca ctaccctggt tgctctctgc cactaccccc ggttgctttg tgccactacc
    58081 tgacagtgaa ggtaaaacga ttacatagaa attgctcgtt tgttctttcc ctccgattaa
    58141 atatcgaggc tgttttagta acaaaccttt tttgcaaaga gcctcgattg ctctcattgc
    58201 cgtgcttttg tttatttcgc attgcttcgc tatgtgctgg taagaagggt aacactctcc
    58261 gttatcgtta gcgttatcag ccaactttaa cagcaccagt ttgtgcattg ggttgccgac
    58321 ctcagtatgg aatacctcga ccattagtcg catactcata aatattggcc tccttataaa
    58381 ctaaaacatt aactgataaa cacagaagaa acctggcagc tgactgatca ggccgcttct
    58441 tttcagttga tgcagtgcag agatagtgtc acgtaacgga atttcgcaca ttccggcaat
    58501 ttcttccggc gttacttcac ttttaccgtt gcatccggcc tttgttgcca gtgcaattaa
    58561 gatgagtttt tgcgtcggat cattgttatt caggcgaata tcaaaagcct tgcgcacgta
    58621 atacatattc tttctcctat ggttagagtg aacgcgctgg cgggtatcca attagcatta
    58681 gatagcagtg actgaccgct gaacagatcc cgccgttgcg tgatatttgt ttttcgcttc
    58741 gcagcgacac cggattttta aagagctttt aaggtatgtg ccttttgtgc tttccgttat
    58801 cttttttctt gcatccttgc aagtatggac ttaccttgtc tgcaagttta cagaatggcg
    58861 ctaacaggtc aaggattgaa aattacattt tgtgacttgc atcaaaattt tttaggttat
    58921 agggtagcga catgaaaaca aaatggtatg acttagcaaa acaactcatg cgggcgcagg
    58981 gtatgagtca ggactcattg gcagatctca tggggatcac aaaaggcggc ttgtcgcact
    59041 ggcttaacgg tcgccgcgag ccgaatcttg aagacattgc gcggatcatg cgggcgcttg
    59101 ggcgtcggca attcactgtt acacatgatg gaatggtcat tgatgattct gtttctaaca
    59161 cgttgccggg agcgccaccg cgtgatttag gcagttaccc ggttgttgac tggaaggatg
    59221 tagtaaacaa tatggaagat gcaaggcgat caacattacc acacgttact actagcgtta
    59281 tttgttcaga tgatagttat tggctggtag ctaaaggcga atcaatgaac gcgccgcaag
    59341 gattgagtat accagcgggg acgatgatac ttgtcgatcc gcacgcgcca gctattgacg
    59401 gaaaactggt tatagcccaa ctggaggagg ggcagatacc gacgtttaaa cagttgatta
    59461 ttgatggagg cagaaaactt ttgcgttcgc tgaatccgct gtatccgccg atccctatga
    59521 atccagaatc aaaaattata ggcgtagtgg ttgatgcgaa gatcgtaaac ctgccataaa
    59581 cattagccgc cggatggcgg cttttctttg cctggaaaac accaaaacgt aaacagaaaa
    59641 catgttttat catttaaata tcaatgacat ataaaatatt ttaaaaaagt atacaaaatg
    59701 gattgactcg gtatatcacg gggcgtatat tgcgaatcaa aggaaggagg caggaaacaa
    59761 aaaaagcacc ttccaggcac tttaaaaaaa aatcaggctt aacaccttgt caaccgtgga
    59821 gtaactcccc gatgggggac cgaaagcggc actgataaca aggcgcatgg ggtgtctaaa
    59881 gcgtcacatc ccccacgaaa ccgcacgcaa ggcgacgagt cagcttgctc ggagtgagca
    59941 aggtcacacc ggaaacggcg atgattgcgg gattagttga aagatgttaa gccgctcttt
    60001 aacaatctgg tcagccgctg gaagtgcggc aattaacgaa gatgattttt attaagtatc
    60061 atcaaggaca taaggagatc agattatgac agttacaata atgtatggaa aatgtgacag
    60121 taaactgaat tccagggaac gcagaagaat aaaaagagaa aacgaaagaa aatcatcacc
    60181 tgcaatcaat aaaacggaca atgtggataa agccattcgc tttgcaagcg aggaaagatg
    60241 caaacccaat ggcattaaag aacgtcgcaa aggagcggta aaatggtata cggaaaatga
    60301 aagcggcaac tactaccacg caacgcaacc acgccagcta ggggaaaaac cccttgataa
    60361 agtccgttac cattaataca aaatgtaaat aataggaggt gacattatgg tcattgaggc
    60421 gcttcaattc aaacttgcgg tagcggaaat gcttcacgat gccgaaatgt ggagcgccgc
    60481 aaataaatcc ttgtatgtag tgttaacagc gaaggagatc aaagagtgaa aacagagtta
    60541 cacaaaaaac tgtggactat tcagcaaacg ctgaacgcgc cgaaaggtca atacaataaa
    60601 ttcggtggat attcataccg atcagcggag gatattttag aggcggttaa accacttctt
    60661 cagaatgtca cgttaatggt tagcgatgaa attgttttaa tcggtgatcg ctattatgtg
    60721 aaagcaacgg caacgctaag cgacggcgag gattcaattt cagccactgc gttcgcacgc
    60781 gaggaaaaag agcaaaaagg aatgacggca gggcagctaa ctggcgcgac atcaagttac
    60841 gcgaggaaat atgcccttaa cggtcttttc tgtatagacg atgcgaaaga tttagatact
    60901 gacgcatacg ctaagcagac aggccagcag cctcgccagc agaaaaacca accaaaacaa
    60961 caaccacagc agcagaaagc gccgccaaat cctgatgagg tgttagcacg tttttgtgat
    61021 gcggcagcga aagcgcagga cgaaaaaaca ctaagggaaa tattcggtaa atgttggaag
    61081 ctgttacctg aaaattcaga gcaacggcag aaagcgcaag atgtatatgg gatccgcaaa
    61141 gcggagctta acggggagat gggttaatga gtttaaattt aatcacgctg ggcggtaata
    61201 tcgggaatga tatggaagtt cgctacacgc aaaacggaaa agctattggt agttttccgt
    61261 tggctgttac gaatggctac ggtgataata aacggacaat gtggatcact tgcctggtat
    61321 ttggcgagcg tgcggaaaaa ttagcgccac atatccgcaa gggtgggaaa atagtggtaa
    61381 gcggtcgcct ggatgttcgg caatatgacc ggaacgacgg cacgaagggg acggcggtag
    61441 aagtggcggt taacgaattt gaattcatga acgttaacca acaaggccag cagcaaggca
    61501 agcagcagaa agcgccaccg cctcaaaata ataacgtgaa taatccgccg ccgatggact
    61561 tcgacgatga tattccgttc tgattcaata ggttagcgat ggttgattat ttaaaaacgc
    61621 caccgccacc gcgaacaaaa gagcaagttc tcaaagaagc ccgtgatcat atcgatcatg
    61681 ggcttttttt atgcggaacg gcagcggaac ggatggcaaa aagatttagt gacctgtacg
    61741 caaaacaaat atggttcgac aactggcagg caagttttta tccattgcaa aggaaaccgg
    61801 atatgcactg gcctgaatat gtcgatccac gtatgcgcaa atatcgcggg cgcatgggcc
    61861 aggttattaa cgattaatga ggtattaaat catgatcgaa gaaaaagaag ttgataagga
    61921 aatgcctgac acagcagatg actacgttac ttttgatggt ggcctgtatg agttcgatac
    61981 gtctgctggg tggcatgata aatggcctct gcctactcgc aaggaattga atgaccgcaa
    62041 atcattcggc gaagatgccg aacgcctggc gaataacaag tggcttaaca agttcattgc
    62101 ggagggtgac aaatgaacct tgcaaaaatt gccgcattgc tggcggcggt agcggtggca
    62161 gtgatgtatc tcagcgtatc gctttatatt acggtggcga tcatcaaact tattactaac
    62221 atgtgaggcc aatcatgaaa gtaggtaagg atattgtgac tgtcgcgttt tgcattattt
    62281 gcttcatgtt tgtggttgtg ttttgtggcg gatttgctgc gttggttagc cttattttca
    62341 ggggggctgt tgtgatgaaa acatgggatg caaaggctgg tgatcttgtc gtgttgcctg
    62401 aatatcgtga tgaccctggc ctggttgtac tgaacagatc tgattgcaat atggcccgtc
    62461 cgattctggt cagatatttg gatggggtga ttatagttcc agtttacttt gctcgagttg
    62521 agctaatggc tcgcaatgta cgcgttaagc cattccgggc ttatgttgaa gaccactggc
    62581 ggaaattgtt cgccggactg aatgggatgt acggcgtatg gctataaaag aagtaaaggt
    62641 taaagacctg aaaggcggtg agcgattaaa ggcgtggtgg gggcgtgaat ttacagccgc
    62701 tgccttcatg atcatgcagg gcggggaagt cgttatatac gatgaagacc tgaaggaagt
    62761 cggcagatgg catcttgaac aatatatcga ggtgttagat gaaaattaaa ttccataaag
    62821 catatgacgc agaaactaac aagctatcgc tatttattga gttcgaacgc cggatcgtgg
    62881 ttgttcctta ttctcggcgc tttaatgatg cggcagggcg gcgtcaattc gcgttagaca
    62941 tgttgttacg tggctgcggc cttatgcgcc cgctatctga tttaaaacgg atgatgcctg
    63001 gatcattcgg tcagattgat gaaattgaag taagccatga agagctaaaa cgggcgcgtg
    63061 atttgttcct ttcaagcgaa ggaaacccgt ttaatccaga aactgaaatg aaatggcatc
    63121 acccactata aaaggataaa tatcatgaaa gacatcaaaa cggcttattc actcggcagc
    63181 gatggcatgt taatcactcg ttatgctgaa gatgcaagtt ttcatgaagt ggaaacgaag
    63241 gattatcacg aagtattaaa agatatggaa gctggagcgt atgacgctga tttgaatctg
    63301 gcgttacaga ttgttgatat tatgatggat gcatcgatcc gcgattatgt gtctttaaac
    63361 gctgaagaaa aaacggctgt agctcgttat gtcttctgcc taactttcgt gaaacgaatg
    63421 gaagaggaat atggtcgcgt gccagtgccg gaagaaattg acccgctggc gttcggaagt
    63481 gctgttattt tcccgttaga tgaaaatcat acgggtagcg tttctctgca ttctatgcgc
    63541 ggcgtaatga aagaaatatt tgaggctaaa gcgttacaga agtgtattga tgaaggacac
    63601 aaagaggaag acgtaaaggc gatcatgcca ttgttttacg gcgaaatggt tggcaataat
    63661 atgcgtgcaa atgatttggg tgtacaggcg gctatagctg tgcttaatga tgtgcgtaaa
    63721 aacgtacagt caatgcctga accggaaaaa cgcgtactgc attaacatcg tgattgctat
    63781 cacatgagta tacattttgt gttgtgattg catatccgtt ttgtaaactg aaattaaagg
    63841 tttacagcac ggaggaaagc aggatggaac gtgaatacat gctttattgc gttgatggtg
    63901 gcgtaggtag cgatgcctac gtcgccggga aaagctatcc agccgatgaa gtgctttcta
    63961 atatgcactt ccgggaaaat gaaaaccagg tgtctgtgtg gaagcgatgc gttgatggca
    64021 ttgaggtggt tagccttgaa agatatttag gtaaatttga ctacgcattg attgaggctt
    64081 aactatggac gaatcactgt tattcatgtg catttgctgg ggttttattg ccttatgcct
    64141 gtttatcagg tggtttattg aataccatat gaggtgattt catgaaaaag gttggcggct
    64201 atacattgcc ggataacgct aaattagccg ggatcggttt tagtggctgt tattactaca
    64261 acattgcagg agatgtttat catgtatact ttgtcgatgg tgtacagcat tttcgcagat
    64321 tgccagatgt cgaattgatc aaacttatcc gtgaggtgtc attacatgcg gtttaaacat
    64381 tacagagaat ggaaaattcc agaagcagcg acaaaggccg cgcctggtaa tttttctggc
    64441 gtttattttt atatggatgg taaatggtat ttcggcagca ggccggatca ctattatcag
    64501 gaaatatgca aacctcatgt atgggatata aaagagcggg tgaaagacgg ggtaattgag
    64561 gacgtttaaa tgataagaga agtgtttcgc tttttgatag cgtggctttt agccgcgctt
    64621 tttattttcc tggcgtgcgc cgttgttatt gccgtcctga tcgtcggcgc aatgtttatc
    64681 acatggtcgc ttcctgatgt tcggtcgtgg actgaattgc tttttggtat acgcgtgcta
    64741 ttcgctttta gtgttttcgt gggtttttgc tgggttgcct cgcctgaatg ggatgatccg
    64801 tggtgattat atggctttat ttaacctgtc agagccgcaa tttaacgccg ttaaaactgc
    64861 cgcccgcgcg gcgctttctg cctgcaaagc agaggtagaa aagaacggct acagcgataa
    64921 agccacgcgg ctgatattag ataagcatta tcgcaaggtc gccccgctaa tcagtattga
    64981 gcgttttgtg tggttggtgg ggtatttaaa caaccgctgg ggagcggttc aggattattt
    65041 ctaagggggc gtaatgaaaa atgattacgg aggcagccat acgccaaaag aaataaagga
    65101 tttatggcaa acgccaaaac cagtttttag aggaatggat cgggaattcg aattcgtcgc
    65161 ggatgtggcg gcaaacaagg aaaatgcgct agttcctcgg tatataacag aagaaatgga
    65221 cacgctgcat tatcagtggg gagcggtggc aatgcctggt gaatatgtct ggatgaatcc
    65281 gccatactcg aatccggggc cattcgttga taaggcggcg ctagaacata aacgaaatca
    65341 cataggttgc gtaatgttgt tgcccgctga catttctgtt ggctggttta tgaacggcgt
    65401 ggagacggca aacgaatgcc gattaatcac gcgcgggcgt ctggcgttta tcaatgctgc
    65461 gactggtaag ccagtaagcg gaaacaacaa aggcagcttg tttttgatct ggcatccacg
    65521 gtgcagacat gaatgtattt ttacgcagat aacacgtaaa gagctatacg caagaggtgc
    65581 agataatgac tagtgcagca gatttattac gacttgcagc ggaaacaatc gagcaaagag
    65641 gtaaacaaaa cggctacgac agaaaagaag aaaaatcagc gccaaaaata gccactatat
    65701 acaacgctaa gaagggggca aatttaacac cacttgatgt atgggatctg ttgatttgtc
    65761 tcaaggaggc gcgtttagag gccattttag ttaatgaaag cgatccgacg gacactctgg
    65821 ttgatttaat cagctacagc gcactaaaag cggagcaaat attaaacgat caggaggaag
    65881 agaaaaagaa aaaacagttc aatgccgcca atattgctga tggaataata aatgatgggg
    65941 ctataacaag ctgtaaggga ttgccgcctt caatgcttaa ccctcaatgt ggctgcattc
    66001 caactagtgt cggcgtgaag tttaataggg ataatgctat tcgtgacaag aggtttgacc
    66061 caaatgaaaa aggtgacaat tgacaggtta ttagcctgcg tttatgtcgc cgttttcgtg
    66121 atcattaacc ttattgttaa ccattgcggc ccgtgggtaa ttccgatcac cacggtagcc
    66181 gctgtgtgtg tcaatatgat gatccgtgac ttcttgttat atgacggcgg cctgaaatgg
    66241 tcggctacaa catgcgccgc cgctggcgca atcacggtaa tgataaatta cgatgctgga
    66301 atggtggcga tagcgtcatt cgttgcggtt gtttccggtg cgcttatttc tggcggcgtt
    66361 tacagagttt tacctggtga cttcgattca aaacgctggc ctgcaaatat agcgtcagcc
    66421 attggcgatg cgttgatttt ccctacgcta tcgttcatgg cgtttatgcc ggaaatatca
    66481 gcgatgcaat tcatctcaaa aatggcagcg gtaacggtga tcactatcat catgcgccgc
    66541 tatttcacgt ttgagggcag aaaatgagca gggctaaaca ttggttaaat aattggctta
    66601 gaagttgggt tgtatggtcg ctgtacgacg gcagcgggta cgccgttaaa gattgggcgg
    66661 aagcaggata taaatgctat tgcttcaact atgacggcgc aaatcatggc gattatgaag
    66721 gcgttaaaat cattcatcca aatattgaat acgttaacgt atggattgac agccattttc
    66781 tggtgatgtt ctcccctgaa ttgtctgttt atccagaccc tgacattatc tttggctttc
    66841 ctccatgcga cgatctcgcc gtatcaggcg ctcgatggtt ctctgataaa cggaaaaaag
    66901 atcctcactt ccaggtgaag gcggcatata acgcaaaaat tgtagaaaaa ctggcgaata
    66961 tgtataacgt gctgtggatg gtggaaaatc cggtgggcgc actgtcaacg ttatggcgta
    67021 aaccggattt tatcttcaat ccttgcgctt acggtggcta cctgccggaa gatgacaaac
    67081 atcctgcttt ccaggatgtt tacccggcgc gcgacgcgta tacaaagaaa acttgtattt
    67141 ggtgcggtaa cggatttaaa cagccgcttt tcagacctgt agatcttaac tctggtgata
    67201 accctggatg ggcgaaaacc ggaggcagga ctaaacgaac aaagatgatc cgctcactaa
    67261 cgccgcgcgg ctttgcccgt gctgtattcc ttgctaacga ccgggccatc acgcgtacta
    67321 cgctcaaccg cgttttaccg gactgaaatt tacaaaatgg cgtcattgcg tgatgcgggt
    67381 cacataatgg cgcttttcac cttgttctga agtatccatt ttgtatgctt caaacaagca
    67441 aaatgctctt taaaaatccg gcagcgctga aatgcgtaga aatcacctga aaaggagaga
    67501 gcattatgcc ttttgataat taccaatggc atgacgacta tgaacgagaa agcgtcatgc
    67561 gtgcaatgtg caatgtatgc agcacgaaga aaggagactg caacgagtgc aacgaatgtt
    67621 tagatcattg gctaaaggcc ggacacgccg aaaggctgaa cgaaacagag aatcaaaaat
    67681 aatcggggtg gtttttatgc aaaaccctaa aaaacccata agacgccgtt gcaaatgctg
    67741 cggcgttttt tttgagccta aatatcacaa tcaaacgtgg tgcagcgatg aatgtctgga
    67801 agaactgaaa tttgatcagc tatgtcgcga tcgtgagaag gctatgaagg ctatggagag
    67861 gaagaaacgc cgtgatagcc agcgtgagga acgcaaccgg aagcggaagc agttaaatcc
    67921 gcgcagctac tggattaaac aggcgcaaaa cgtttttaac gcttatatac gcgagcgtga
    67981 cgcgctattg ccgtgcatat catgcgggac ttactacggt gagcaatgcg ggtgggatgc
    68041 aggccattac aggacggtag ccgccgccgg acaccttcgc tttaacgatg ataattgtca
    68101 caagcaatgc aggcactgca accagacact agacggcaac atcggagggt atcgccctgc
    68161 gctgattcga aaaattggcc tcgccagggt agtagctctg gagaacaaca acgaaactca
    68221 caaatggacg atagaagaat gcaaggaaat cataaaagtc tatcaggcta aattagacgc
    68281 cttaaggaga aaggcagcat gaacgaatat acatttagcc taccataccc gccatctaat
    68341 aaccgctatt accgacattc acgcggtttt cactatatca gcaaggttgg taaagaatac
    68401 cgtgagcaag taaaagacat catcgagcta ttaaatttaa acatcaacct atcttgccga
    68461 ctggcaatta ccattcatgc cgcgccgccg gacaaccgga tcagagatct ggataatatc
    68521 cccaaatgcc tttttgatag ccttacttac gccggattct ggaccgacga cggacaaata
    68581 gactcaataa aaattgtacg ctgccggaag gtaaaaggcg ggcgattgtt tattaagatc
    68641 cgcgaacgcg gcgacctatt accggatatt gacgaatacg cgactaatat gtggggtggc
    68701 aatggagata ataaacattg ttgttaaatg tactcaatca agcgtcgaat ttttcactga
    68761 aggtaaaaaa tattcgggtt ttataaagaa tgggtgcttt gcttttattc tacaagacga
    68821 tcttgtatct gatttcgacg acgccgatgc ttggcgatta gtacagtatt tcccatcaaa
    68881 aaatgagtat ttcttacagg gccattttaa aactcagttt aaggtggtag aaaatgaaaa
    68941 acgaaattaa agatctgcaa atagatattc agcgcgatga gggtgatttg gaaacggtac
    69001 gacaaataca ggctttccac atgcgagaat tgcttgcgct gaaagagttt gaaagaaaac
    69061 ttgtgcaagc gatatctgat cgcaaaagac tggttgcgcg ttacggggga aaataatgaa
    69121 tttggaatct attcttaaat tccacttccc aaaatcaccg cgtttgtcag atgaaagccg
    69181 tggcacgtca ccggatgcgc ttaatactac tgatgcgcta actgccgcag gtatggcgca
    69241 atcgcgcgta gagcttggtt atagcgcttt tttagggaag atggaattat cacaagccga
    69301 aaaacacagg gccgtagtat tgcttacaga gcgtttaagg gctatggcaa aagattataa
    69361 atacgttatg gagctaaacg aggcgcaacg caatgatctc attattcttg ttgccgtttt
    69421 cgcgtttcgg gattattgcc agagtgccgc gacggaaaaa acttgtatgg agtgcggcgg
    69481 atataaattt acgtttgacg aatacggaga aaaacacctt tgcaaaaaat gcaaggggca
    69541 tgggaaggtt ctcaatcatt gtaaacaatg caagggccgt ggcgaagtac cggataaagc
    69601 agccagcgaa gcggcggaaa tgccagtgtt caaaacgtgc cagcactgcg gcgggcgcgg
    69661 gtactcgcgt ttccctgtag atcttgtccg tcaggcggtt aatcagcttg ttttcccggt
    69721 aagccgatca acatggtgga agaaataccg ggttttctat gaggacgcca ttgctgaatt
    69781 gttcaaagaa gaggcgcggg ctgataacga aattaaacga gtgacgcggg gtgaatgatg
    69841 gataagctgg aaataaacga tcagtttgct gttatcttgt tcgaagataa aacaggcgga
    69901 gcatggtgta agaaagtcac tggtgcggaa gcgcgattaa tattgggtat ggcaagcgcg
    69961 ttaaatgatg gcgaattgcc agccgtacca attgagccgg tgcatattta cggacggagt
    70021 aacgacaatg aagcctaaat taaaagaaca tctatttgca aagctggtta atgacctgac
    70081 attatccgcg cggttgtgcg gtgatacgca acaattgagg gcatggatag caagggattt
    70141 aagaaaatac attgagcctg gcggtcctgg cgacgaaatg actatggaca aggccatcaa
    70201 acgccgttcg gctgattggt cagtgagcaa tacggggtta accggatata ttgaaggcta
    70261 taacgactgt ttacaggatc atagcgatgg caagtaaaga cctacatttg aacctgaaag
    70321 gtgaatattt ccacgccata ttgaccggaa agaaggtgga ggaataccgg ctttataatg
    70381 actattggcg taaacgtctg gaagggaggg aatatgagcg cctgattatt aaatggggat
    70441 accctgccaa ccacgaaaaa cacaggatta ttaaccttcc ttattttggg tacgaagtga
    70501 aaactatcac gcatcaatta ttcggccctg atcctgttaa tgtattcgca attaaatgta
    70561 acagaaattg gatgttgaga ggttagaagt gaaaagtaac cgtaagcgcc ttgtaagggc
    70621 atacgacaaa gcattaaagg cttttgatga tctgcgacgc aataagcgcc agcgccgtaa
    70681 atgggcgcga atgcttgtta gtgagtggca tgatgaggat tttttccttg aagccagaca
    70741 catgacgcag gatgtagcag acgcattggc tgacgagaac gtttattaca tgatgtggtg
    70801 acaatatgca aataatcatt gattatcttt gccatgctgc aaatacagtt tttggcatgt
    70861 atcaacaacc attcctgaaa gaatgggatg aaatgttaaa tgacatcatt gataaaggat
    70921 taatagttga agtaggcgag ctaacgataa aatttaatta cgaaggcaaa gaatatgata
    70981 tatgggtagg taacagatgg tacgcatacg ggcatattta ttcaattggc ggcaaataca
    71041 ttaaacgtag acaggaattc aggccgcgat tccgcacaat gcgccgcctg cgtgacctgc
    71101 atatgaatat atttgaaaat caggaagcgt gtgaactatt caagatctac ggtgataaat
    71161 catggaatta aaaatctggt gtgctatcga cgttgttgat aatgagttgt ctatgttcgc
    71221 aactgacgga aagcgcgtgg tgatcgctac atggacgcgc aaccacgatg atattgcttt
    71281 tcgtcgcaag gctgcggagt ggcttttctc tgatggcggc tacacgatga acatcgcaca
    71341 gcttgccaga atgaaagatg aaatactggt ggacagctac acaactgcat aacggggtga
    71401 atatgcgtat ctatgagcac aagcgggata aaacccgctt ttttgttcgt gctggcgtgg
    71461 cgtaccagta tcacgaatgc ggttacattg aggcgcttgc ttacgatctg gacttcgagc
    71521 aagaaaaggc atggttcgat tttaaaattt accgtaagcg caaacctacg cgcgacgagc
    71581 gccacgctat ccgggacttt ttaatcagta ttgagcgatg ggagtcagat taatgaaagt
    71641 caaattcttg cacgatcacg gttatccgtc actgaaacag gttgttggta aggtggttaa
    71701 cgtcgttcat agtgataatg ttacttgcat gattaacggt gctgacctca ttgccgctgg
    71761 cgctgatgac cattacataa atccggcatg gtcgtatacg ttcagcctgg gtgacttcgt
    71821 tggcgataag gggcgcgggc tgcaagtggt cgagggttaa tatcatggac gtttacgaag
    71881 atctatacct tcagacgaat acccacactt tttattttct gaaaaactgc gtcgtatatc
    71941 gcagcgacga cggggtaata atgaaagagt ggttatttaa gcgtaaagac ctactcgacg
    72001 atctggtttt tgccgggata tttcgtaaac gtccggctaa cctggaagaa gaaatgttga
    72061 ttgaggtaat taaaaatgaa aatcagggta agttatttcc gggcgaaaga taaagcaaca
    72121 ggaaagcaga tggcgatcct ggttaacgag gcgcagtaca tgtttgtcct tcagccgtgg
    72181 tgtgttgtag atcttaacga caactgtcgc cgccacgctg cgcgaagtgc tgttggtatg
    72241 aaaggctggc aaccgcgcga catggaaaac tattgtgact ggaaattagt agcaaaatac
    72301 acggtagatt ataaaggggt tttctgatag tggtgaggtg gtgttatgca ggttaaatgt
    72361 gtcgaatcaa atagcgacat ttttgtagtc gataagattt atcacgtcta tcaggttttg
    72421 ggcgttgact gcgatgaaag agatcaagtg ctaattgatg aagatggcga cccgtggaaa
    72481 ttcctatatc gctacgccag cggcggcgat gttattggta ttcgccttcg tgcaaaattt
    72541 gaactatgtt aaggtttaat catgaaaagc gataaattca ttgcgtgttg ttttgttgaa
    72601 gtgcctaaag gtgatgtttt ctgcaattat gaatttaaag acagtttagg cagatctcat
    72661 gttgttgttg gtaagcgcgg agactggtta tatatcactg atggtgacag gatcttcacc
    72721 aaaaaccagt tattcagaat aaaggcagaa aataatgaaa tacctgtagg caatatgggg
    72781 attatgaact accacgcctg cctatgttta aagcatgacg cggtgaataa agcgcgtagc
    72841 ttacctggtg ctgaatttta atttattacg aaggagaata gcaagtgtct aaatttatca
    72901 gcgttaaggt tttccgtggc actttaccga acgaagaaag attggggcaa ttcgcagggc
    72961 aacctggtgc atgtttccgt gtagccgctg aagacgatgc gcacgttaaa tgcttccacg
    73021 tatcagaacc gccttttaat gcagacgatc ttgaaaacat ggatagaatt aaaggcctag
    73081 tttttgccta tctgatgttc cagggtatcg ctgatatgga agttgaattg cttagcgctg
    73141 aactagtcgc agaatacaaa atgactgaag cagaagaagg gggaattaaa attgagcgca
    73201 tcaaataagc tgtataaagt caggtgtaaa ggcgaatacc ctggctttac gattggatgt
    73261 gaatacctgg gccatatagg gtacggccat ttcggtgaat tggggatgaa tacgatggac
    73321 gacgacggag acagcatgac gcttgatctc gattctgacg atttcgaata catcccgcca
    73381 gtaacttgcc gcgtagttga tgagttcctg gctgaacatg aagacgatga ggatgattat
    73441 gattgaatgc cttattgctg tgactgtttt gctttatatc gctggcgcga tcctgatgtc
    73501 atgttttatt aagtttagcg gcgctcaacc tggtggccta gagattcctt tatggcctgt
    73561 agttgttttt ctcgccttct ttgatgctac ggcagagcaa atcaggcgag ccattaagga
    73621 gcgcaagaaa tgattgaaga tggaatctat ggtgtaaacc tgatggaaaa cgttttttac
    73681 atggtggaag gcgataccgt aagaatcagc atagatggtg gggaatatgt agagacattc
    73741 ttcaaaacaa caaaggaaga tattaaacta ggcgttgata aaggcgtact atttaaggtt
    73801 ggcgaactat gaatgagatc gaagatggca tctatttgca caaggtattt aacatcgttt
    73861 atttactgaa aggtgataag gtgatgataa gacctgatga tgatccgtct tgggaattaa
    73921 gcgacatgga tcggtggcat atgcagaggt tgcttgataa cggcctgata tacagaaagc
    73981 agtaaagccg tatttgtatg gcgtaaacaa acagattaaa atattctcta acaatgcgaa
    74041 actgtaacta cccggccaag tgccgggttt ttgctttgtt ggaggtaaat ctatgttcga
    74101 cagaatacgg gcggcgtgcg cttatgtgac tgggaccgta accgcttttt tcggtgcgat
    74161 aaccataaat gacgttgccg tcttcgtggg tatcctatca accattggca catttgccgt
    74221 taattattac ttcaaatcac aggagaacaa gcgagcgcaa gaggaacacg acgcgcgaat
    74281 ggggaataag taaaatgatt agccaatcgc tgaaaaacaa gattgttgcg gcggcggctg
    74341 gtggggctat ctctattgcg gcggtgatga ttaagccatt agagggcgtt gactacgatc
    74401 cttatcgtga tgttgttggt gtatggactg tttgctatgg tcacgccgga aaagacatcg
    74461 tgttgggcaa gacttacacg caatcagaat gcgatgcttt gttaaataaa gatctacaca
    74521 agaccgcaaa agcgattgac ccatatatca aagtcgaagt atcagatttt acccgcgcag
    74581 cactttattc attcgcctat aacgtaggcg caaccaactt caaaacatca accttattaa
    74641 aactgctcaa tgacggcaag aaatcagaag cgtgcgcaca gcttaagcgc tgggtatacg
    74701 ctggcggtaa gaagtggcaa ggcctggtaa accgccgcga ggttgaatat gccgtttgcg
    74761 aatggggtga aacatggaca aggtgaagac gttaatcatc gccgctgttg tttgcatcat
    74821 tgccagcctt acagccgtaa catgccatta ccatagtgag gcgacaaggt tacaggaaga
    74881 attaacgacc acgcaaggcg cactgaaaac agcaagtaac actattcagc agatgaagga
    74941 gcgaaacgcc gaactgtcaa aacttgataa gaggtatcac gatgaaatta aagctatcag
    75001 atctgacatt gccgatctgc gcactggtat tgataacggt actatcaggc tgcgcgtcaa
    75061 cgcaataccc gtgcgagtgt ccgactccac cggttccgcc agcgccatta atggagcctc
    75121 ctgtagactc actcccgacg ctgaatcggc ttatctatcc ctcagagaac aactaaaaga
    75181 aaaagacgcg aagataacag cgcttcaggg atacatcaaa acgcagtgct tacgcaaaga
    75241 ataacagcgc gtggacgtgt cgccgtttct gccagccagc cataaccggg ccaatccttc
    75301 ccgcgagcga cggcggaaat tcaaaaacac gtaataccgg atgcacgtca tcccatttac
    75361 aggtctggcg ctacctgggt agaagaaagc gccatttact cacctgccat gcgtatgacg
    75421 agcgcccgga tctgtttatc agagatgtta agcaggtcca tatttataaa attctggaaa
    75481 cggtactcac acagcgccgt tttcagtgtt ttatgtcatt cctgccgctg tataaacagg
    75541 aagtaaaagc aaaccagaca ggagattatt ctgatggcta aggctaaagg cattaagttg
    75601 cctcaattca aagtcccgct ctttgagcat acaacagttt tcttttgccc gacccgcgat
    75661 atgttttatg aattttgcga aaaggcaggg atcccaatcg aacctgattt cgacctggca
    75721 ggagggttga cgcttacttg cactggagag aaaggcggta acttctacgt gatcgcagta
    75781 ttcgacaatg agttaggaac gctggtacat gaatgcgctc acactacatt ccatgtttta
    75841 agtgatgtag gcgtcgtggc tactactgat ccaactcatc ctgcgaacga gacttacgct
    75901 tacatggtag gccgtatctt tgatgcgttc ttccccatcc tggcagaatc aaatgaagca
    75961 caggtggcag caatgcaggc ggctgaagtc gttgagcagg cattagagca ggcagaacag
    76021 ccgaaggaag aagaaaaacc agcgaagaaa ggcaaacgta agccgaaagc aaaagaagcg
    76081 cttgtgccgc gtgttatgag ctttaaacgg ggttgattat gcttactttc ctgttaggtt
    76141 tatttatcgg aatggctgcg gctgtaatat tgctggttgg cgagtaatga catggacact
    76201 tatgtaattg ccggattgat cggcgtttct gtattcctgg taagttttgt tatcgctgta
    76261 ctgataaccg taaaaagcaa atcatgaatg cctatgttct catctggttg ttggttctca
    76321 tcgtaggtat tggcgtgaat atttacaggc tatggtaaaa atatgattga cccgctcgtt
    76381 atcctttctt tatgcgtagc tgtatggctg gcgatcatga tattcattga aagctaaagg
    76441 tattcacatg aatatttacg atcttatctg gtggtcgttg gtcgccgtaa ttatctactt
    76501 ctggtgtaag aatgttgtat aggtgaatta tgtatggccc attctacgag ttaataccgc
    76561 ttgtttttct ggcgattgtt atatggtttt tctatgcgat gttcagagat cgcccatagg
    76621 tgacagcatg gatatattcg aattactggc gattatgtgc ggaatgatgc tgggcgttgt
    76681 cgccataatc atcatcattg gtctggttat aggtagtgtc aatgaacgca tatgaaatgc
    76741 tattgctggt ggctgtagtt gtagtcattg ccgttgattt ctatagaggg ttaaaaagat
    76801 gagagaagca gacttctttt gcatgattat agtgatcgtc atcgttgccg tgcttttaag
    76861 cgttaactga atcgcagggg gttaaaaatg aaatggcttg atttcttttt cctgaacgtt
    76921 gcgattgttc ttaccatgac cgcgctgact caataggtta tggcttggac gcgcatttta
    76981 tgcgcttcct aaattaagca agtcaaataa agtggatgac aacaatgaga gagctattcg
    77041 actttctgga aacggtaaca tactgcgcaa cattaattgc gtgtttatat attctgtgta
    77101 aatggttttg agaggtgaaa tatggtccgc acgaaaaagg caaaggctga cgataaaaag
    77161 ccagcagcca aaaaaactgg ccgtccacat ggctatacag aagaaaaggc attagagatc
    77221 tgcgagctag tgtcggacgg cgaaagcatt aacaagattt cgaagatgcc tggtatgcct
    77281 gcgcgttcaa caatccttaa atggttccgt gacgtgccag agttttcgga catgtatatg
    77341 cgcgcgaaag aaattggttt tgaggtgtta gctgatgaga tcatcgatct tgctgatgcg
    77401 ccagagaaca ttaaaaagga agagttgaac agacaccatt tgatgattga aacgcgcaag
    77461 tggttactgg caaaactgca accgcgtaaa tatggtgaac gtgtcacaca ggagattgtc
    77521 ggcaataggg atgaggcgcc agtacaggta gaagtgacga aagaagagat cgcccgcatc
    77581 gtccaggaag tagaagatga ggtgtgatta tgttgacaat caaagaacga gttattcagt
    77641 ccaaatgtga aaaggatggc ctgttcttca atcgctattt ctataagcaa gcgaacggaa
    77701 cgaagatgtt aatttcaggc catcatttag ccatccgtga tgcactgcaa cgcgttatta
    77761 atggtgagat cacccgcctc atcattaaca tccccccagg gtacggcaaa accatgatcg
    77821 cgactatcaa tatgatggcg cggtccctcg caataaatcc ccgcacaaga ttccttcacg
    77881 tttcctattc caacaacctg gcattactca attcctctac tgtaagaaac atgatctgta
    77941 cgcctgaata tcaggcaatg tggcccatga agatccgcaa cgatgccaac tcaaaatcga
    78001 tgtggtggac agaatacggt ggcggcgtgt atgcaacgtc atcacttggt caggtgactg
    78061 ggttccgtgc ggggtacatg gaatcaggat ttaatggcgc gttaatcatc gacgacccat
    78121 taaaacccgc tgacgcttac tctgacgtgg tgagaaagca agttaacacc aactataacg
    78181 acacgcttgc ttcacgtctg gcagtgcaaa caactcccgt catcgtcatt atgcagcgca
    78241 tccactacga tgatttgtcc ggctacctgc tacgcggtgg gagtggtgag aagtggtatc
    78301 acctgaacct gccagtgaag atagacaata gcatggacta ctgggatctg taccctgaaa
    78361 acgagttcgc catccctatt gctcacaacc tgccagatgg ctggctatgg cctaaaaagc
    78421 acaatgacat acacgaagcc ggactgaagg cacacagacg gtcatttgaa gcacagtaca
    78481 tgcagcgccc gcgtaaattc gacgaagagg gtgcattgtg gactgaagcc atgataaccg
    78541 cagcgcaccg gatgcagata acgcaggata aagtccgcac ggtgatagcc atcgacccgg
    78601 cgacaacatc atctgatgag tcagacgaaa cggggatcgt ggcatgttcc gcttatggtg
    78661 gcggcaagaa tgctcagtat tctgtagacg gtgactactc aggccgcatg tctcctaacg
    78721 actgggcaca ggcatcaatg aacgcctaca acatccatga agctgacgcg atagttatcg
    78781 aaacaaacca gggcggcgaa atggcagagg ccacgctacg caatgccgga tttaagggcc
    78841 gcattgttaa ggtgcacgca agcaagggta aattcgcccg cgcagagcca atatcggcac
    78901 tatatgcgca gggaagggta gcccacactg gcagcctgta cacgctggaa aatcaaatga
    78961 tggaatacgt gccagccacc gccaaaaaat cccctgaccg tatggatgcg atggtgtggg
    79021 gcatcactga attaagccaa ccacaggcaa tgggcctcat gttaccgaag cgcctgcgcg
    79081 gattttaaaa tctgactcac aatccccaac aagtttttct atttttcgcg tagcaacgcg
    79141 taaacatgta ttcaggagta aacattatgc catcaaattt agatttggcg gttaatgctg
    79201 ccttgtcaca acgccaggcg gcctatgccc gctatgcagc cgccaaccca ttcactatgg
    79261 ggatagatgc aaaacgtgat tctgcatgga gtgaatacgg attcaaagaa gagatcacgt
    79321 atgaagacct atacaaactg tacaggcgcg gcggtattgc tcacggtgct gtagaaaaga
    79381 ttgttacaac ttgctggcga accatgccga cgctgataga aggtactgaa gacgagaaag
    79441 ccgaaaagga aacaccctgg gaaagagaaa tcaagaaaca attcgacaac agattttggc
    79501 gcgtcattgc cgaatgcgac cgccgccgcc tcatcggtcg ttatgctggc ctgttgattc
    79561 atgtcaggga taaccagccg tgggaaaagc cagtcacaaa aggcgtaggt attgccaaat
    79621 ttaccccggt atgggctggt gctcttacgc cgaaggagtt cgaagaagac ccggacagtg
    79681 aaaactatgg cctgccgaca tggtgggaat acaaagaacg cattaacagt aagaccattg
    79741 ccagaagaat ccaccctgac cgcatattta tcttcggcga ctattctgat gatgctatcg
    79801 ctttccttga gccgtcctat aacgcctttg tgtcacttga gaaagtggaa ggcggtagtg
    79861 gtgaatcgtt cctgaaaaac gctgcccgtc agcttgctat ctcattcgat aaagagatcg
    79921 acttccggtc actggcagcc acatacggat gcgacgtcac agagctgcgc gaaaaattca
    79981 acgaggcggc ggctgaaatg aataagggta acgacgtgat gatggcatta cagggggcaa
    80041 cagttagccc gctggtgact gcagtatctg acccgtcagc aacctatgac gtcaacttac
    80101 aaaccgccgc cgctggtatt gatattccaa cccgcatcct ggtagggaac cagcaggggg
    80161 aacgcgcatc aacagaagac ctccgctact tcaacagccg ctgtatgacc cgcagggagg
    80221 aaatcggtgg cgagcttgaa gatctgttcc gcaagatggc agatctccgc cttatcagta
    80281 tgccgattga cgtatcagta atatgggacg acctgaacgc catgaccaaa gccgaactac
    80341 tggaagcggc acacaaaatg gcgcaaatca atcaggcttg cctggctact ggtgaagaaa
    80401 tatttagcgg tgatgagatc cgcgaggctg ccggatacga tggccctgcc agtgtagtag
    80461 aaatggaaga ggaaaacgat gatgaaggtg aagaagataa tcaggcgaat acctccagcc
    80521 gcgataatgc catctaacac cgaagacccg accatgacag ggaagctaag gtctggtgct
    80581 atcaagcgtt ttaaagcctg ccttaagaaa gttgcggatc cgtatatcgc catactggac
    80641 aggatgcaat atagccttgc tgttaataaa aaatacacct ttcagatcta cattgacgaa
    80701 ttacatgact tgctggaaga cgccagcgac atgattgatg agatattcga gttaacagac
    80761 ccggaaaact tctggttctg gcaagaatat gtgaaggtgg catatcaacg cggcacttct
    80821 caggaatacg ccaacctcgc taatcagtca gtcacatatt ccagagctta tcctgaagtg
    80881 tcggcggtat taaccagcca gacctatcgc acgcgccttg ctctggtgcg tacccgcgta
    80941 tttgaagaga tgcgcgggct gacagcacag atcaaaaagg atatggcccg ccgattaact
    81001 gaaggcatgg cccgtggttt aaatccactt gaaatagcgc gcacattgca gcaggaaacg
    81061 caattaccgc tatacaggtg caaacgtatt gcccgcactg aaatatgcac agcgttacgc
    81121 acagcgcgta tggatgaggc agaagcggcg acggaagagc ttaatttgcg cactatgcaa
    81181 atgcacattt cagcattgtc accgactacc aggctgtcac acgcacagcg gcacgggaaa
    81241 acgtacacca tagaggagca gcgcgaatgg tggagcagat cgccaaattc aattaactgc
    81301 aaatgtagca ctattaccgt gctggttgat gaatctggta acatattaaa caaacgaata
    81361 ttagagcggg cgcaagaaaa ttataaggtt gcgcacgcta aatatggcga agattgggag
    81421 taataaccgt gagtaaagaa ttaattcagg ttaataccaa attaaccgct aataccatcc
    81481 gccgggaaac gtataacggg cgtgaacaca ttgtagtccc gtcgtatacg ttgcccttca
    81541 atatcgttat gaatcgggaa tattacccgg aagctgaaat tatcgctaat taccagtcac
    81601 tggaaggaac acttgccccg ctgggccatc ctacggttga cggcgaattt gtttccgcat
    81661 ttagcccgga aggtctgaat attggctttt gtggggcatg gaacagaaat gttgaattac
    81721 gcggcaaccg tgtttatgtg gaaaaatggg ttgatgttga aaccgccagc cactcagaac
    81781 aaggccgcga attattaagc cgactggaag cgctggaaaa aggagaaagc aaagatccga
    81841 tctggtcatc tgttgccgta tatcgtcagc gtatgccagc tactgaagag atgaaagccc
    81901 agggtgctga tagcgttgtc aaaatcatgt cgattgacca tgacgcaatc ttactgcatg
    81961 aaccgccagc cgcatcgcct gaacagggcg ttggcctgat ggttaacact gaccaggcga
    82021 agccgttaat ggcggtggca atgaaagaaa acagctatcg cacgcttgaa agacaacttg
    82081 aggaagctgc gcgtaaactg ttccccgatg cagattatgt ctatgtcgtg gacttcactg
    82141 ataaagaggt aacgatcgcc actaataccg aaagtgctca agtttgcaca tatgaaaaac
    82201 aggctgataa aataattctc aataatggcg agcttgcaac caatgaggaa ggtaaatcat
    82261 ggttcgttca gttcgctgaa cacttatcta atcttttctc cctgaatgaa aaaattaagg
    82321 ccaataaatc ggagggtgaa cccatgcctt tgaccaaaga agaacgcgcc gaactggtaa
    82381 aagaaattaa cgaaggttta gccgctaata tcgctaatgc agtagcagag gcattaaaac
    82441 cagtacaggc aagcgttgaa gaattacaga ccaaccagaa agcgatcaaa gaagagatcg
    82501 cagcaaacgc aaataaagaa gaagcagaaa aacgcgccgc agtagcaaaa gttcacggcg
    82561 aaattgttgc taatgcttta agcggcgaag cattagaagc aatgtttaaa tccctgggta
    82621 aagcagcacc aatggcagca aactcagccg ctggtggaaa taaacctgct acccctgaca
    82681 ttaacaacta tttcgcataa gaggtgaatt atggcccgtt ttcgtcgtgt aaatattgac
    82741 ggcaaatccg ttactgaaac cgcagtatct gccgcagcac ttaaaccggg tactccggta
    82801 aaaatggcgg ctggtaaatt cgctgccgca actgatacca caggccgtat ttatgttgta
    82861 aatccggcat atcatgaagg tttaggcatt gaagatgcga tcccggttgg tcattccgtg
    82921 gttgctgact acgcagaaga agggcgcgaa tttgcaattc ttcttcctga aggcacttac
    82981 accaaagacg ccgcaatcac cattggtgaa ggaggcttta agttggtcgc atctgatgaa
    83041 ccggttttcg ctttctgcca ggaagctgta acccttgaag cggctgattt tgtacgtgtc
    83101 cgcgttgcat aataataaga ggtgaaaaac tatgttgttt aataaacaca accttgctac
    83161 taacagccgc attcgtgctc agtgggatca cctttgggcg cagcgcaaca tgttcaatga
    83221 tcgtgacggc gctcttattg ccgcaaatat ggcaaacatg accgctgaca tcctggcatg
    83281 taatgccgtt ggcggcttcg atcaggaatt ttggaaagct atcgataacc agattatcga
    83341 aatgagcacc gaagagactg gtattgaaat tgtaaacgac ctgatggctg ttcaaaccgt
    83401 attgccaatc ggtaaaaccg agaaaatgta cagcgtatcc ggtgatatta acgatgaggt
    83461 tgtcgttagt atcgatggtc aggctccgca tggcttcgat cacaccgaat acggtagcga
    83521 tggcgacccg atcccgatgt tcgcagcggg ttacggtgtc aactggcgtc attccgaagg
    83581 tctgaaaact gttggtattg atcttgcact ggatagccag cgcctgaaac tgcaaaaatt
    83641 caacaaagca cgcgttgatt attaccttaa cggtaacgca aaaatcaacg tcaaaggtaa
    83701 acctgggcag ggtattaaaa atcaccgtaa tacccaacag ctggtaatga catccgaaga
    83761 cctcaccact gacggatttg acgatctcat caaattcttc accactggtg cattcggtgt
    83821 tatggcccgc aacaaccgtg ttgatcagta tgatattatg tgggtatccc ctgaaattat
    83881 ggctaacatg gcagcgccgc acattgctaa cggtactgtt gttggtactg tactgaatat
    83941 cgttaaaccg ttcattccgg ttaaagaaat ccgccagacc tttgcactga aaggaaacga
    84001 gttcattgct taccaacgcc gccgcaacgt cattaccccg ctggttggta tgacgactgg
    84061 tgttgtccct ctgccgcgct ttatgccgac tgataactat aatttcaaaa tcatgtcagc
    84121 agaaggttta caaatcacct gcgacatgct gggccgttcc ggcgtagttt acggtcataa
    84181 ataatttcgt gtttcctgta actccccggc gcgatgccgg ggattttttt tgtatgtgga
    84241 gaaaacaaaa tggttactgc acaacaggcg cgggaatatc tcgaaagcca gggcgtggaa
    84301 ctgccggaag tggttttatc tttgctggtg gagcaggcaa acagcgttaa tgaatgcctt
    84361 gatgccaact atccggcctc cactgcaaca ttgattcaac tttatctgat tggcctgtta
    84421 ggactcaccc aggctaacaa atacgtttcc tcacacactg gcccgaacgg tgcaagccag
    84481 tcataccgct atgtcgattt caataaaaaa tggaaggcgg cctattcgtt gctttattcc
    84541 cttgataaac atcactgtac agccgaactg attccaccgg atccagaaaa caccgcgcac
    84601 gccgggctgt ggataggtaa gagcgggagg atgtgacaat gtggaacgac ctgacattac
    84661 caaaaccgtt attaccgaaa ctgtttaccc gcgtatgggt gaagacagat accgggcgac
    84721 aggtagccgc ctatctcaat gatgctggcg aatgggtaat tctttgcccg cgcgtggcta
    84781 aaacccatcc gaagatcgtt aaatggagct atggctatga gtaagatcgc gagattcagt
    84841 tacaaggcat tagccaccat ttaccccgtg aagcgtgacg actggacaaa ctccgacgta
    84901 tacggaacgc catatctgat tgattgttca tgggaacgca ctgacggaac cgcaacagac
    84961 gcaaacggga atgaggttag caacacaata accgtatata ccgaactgct tcacaagatg
    85021 cagccagtac agcgcccgga aaaagggtgg atgcttgcca ctggcgacac taccgccatt
    85081 gctgacccgc tggcggctgg ggccaacttt ataaccggga tcgttgaatg ggacatgagc
    85141 atgtttaacg acacgccgga ttataagatc gtgacaggag gttaatcatg ccactgaaag
    85201 gcgtcaggcg tgtccgaatg aaaatgactg aagaaatcag gaatatagca gacaaaagga
    85261 cttatgaagt gttgtggatg gttggcaacg ttgcctccgg ccttgcatct ggaatgacgc
    85321 cagttgatac gggtttttta attaacagca tgtatcagac agttgagaaa aacggtaacg
    85381 gtctttgtct cagtgttgga tatactgccc gctatgccga atgggtgcat gacatgccag
    85441 gtactttaat ggggcaaccg cgcgagcatt tcggtaaaac aaataacctt tcagatttcg
    85501 gaccgaaaca ggtagttgag ttcggcggcg gtactgggaa gggttattat tgggatccga
    85561 acgcggaacc tgaatttttg cgcaaggcat tcgaggaacc agataatttc aatgagatct
    85621 ggaacacgat taaacttggg tactggacga aacaatcatg aaacgcagcg aagtatacga
    85681 tgaaataagg gattggatta aatcccacgg gtacgacgaa ggttatattt tgcaggcccg
    85741 tttctggaat gagcgatcca attctaataa cgaccgatat attgttatcc agcaaaatgg
    85801 cggggcggct ggtgaagaag cagtaacccg tgactacttc cgcatcctgg ttatttcagc
    85861 gcgcaatgac gcaaatatca gcgaagtgga agacctcgca gacgcaatac gccaaagtat
    85921 gataaccgaa tataaaactg ataaaattac acacatgaag ccagttggcg ctattcctgc
    85981 aatgcagacg agagaagggc gctttatttt taccgtagct ttccaaacca ttatatccag
    86041 ataagaggta acaaacatgt ctcagacttg cgaaaaaggt tcgtttttag gtcgcgacgt
    86101 tgctgtattc tacgctatcg cttgccctaa tgcgaaacct gaaaccgacg cctataaagc
    86161 gttaggcatg atgcgtggca aatctctttc cgtagaatgg gaaaccgcag acgccaccgc
    86221 tgataaatca gcagactata ctaaagaatc actggtaact tacaaatctg ttgccttctc
    86281 cggcgacggc atatcccgca ccgaagagat ccacaaccag aaggcactta aacgccatgt
    86341 tattaaccct ggcgaaacaa ctggtgcgca gccttacgtc tggctaaaac tcgtttcccc
    86401 gctcgacgta accgaaggtc cgttcctttg cacctccttt aaagaagaag atccgcacga
    86461 cgatgtgtcc acctggtcca ttgagtgcga aagcgctggc aaggtcacgg taggtgacgt
    86521 tccgtcagtg taacagtaaa tattaacatc ggggccatct ggccccttta tttttagggt
    86581 ggcagatatg attcatgttc gaacaggaca atttgcggtc gtggttgatg gcaggcggta
    86641 tgtattcaat ccatgctttg ctgcaatggc taagatcggc agtgacaggg aattagttga
    86701 atacttcgca actgtgcacg gtggcaaata cccacaacgc ttgccaacgg atacagacct
    86761 ccgaaatcgc attctggcgc gttgttatgg tgaactggtg caaacgtcca tccacgtcct
    86821 gaaatgttgc tcagaggacg aaataggccc gttattgggc gaatgtagat ttactccttc
    86881 tggtaagttg atactaaaac ccgggctgat gcctaccggt gacattatca cgctggcaca
    86941 acattgcatg taccacggtt taattggtga tggcccggag gaagacgcga gcgcaaccca
    87001 ggaaggtgag tacaagccta cattcgatgt acttgagttc gtttattctg ccgtagctca
    87061 cctggggtta tctgaatcag aagcgtggga catgacaatg acaggatata gggccgctgt
    87121 acgcgctaaa acgccaccag acgaaagaaa cgagagaagt aagccaaatg ttcacataaa
    87181 taaacgtgat tatgatgagc aaatggaagc cgctaaaagg gtgctggaat tgatgaaaaa
    87241 tagtgagcaa aaaaaagccc ggtagagccg ggcgtcatat gtcgatgatg ctttttgata
    87301 gtgcccatta tctgatcctt acaatttcaa ttctgccgtt acctaaatca acgatagcat
    87361 gattttttac acctgcgccg ccttcgttaa atattctttc agcaaatgcg gctttatcag
    87421 cgttgcactc gttggctatt ctgtttctta tttcattgtt gccttcgaat acgacaccta
    87481 cagcagcctc cttcacaagc ccataattaa accatcttgc ataaactggt ttaccctcaa
    87541 aatgcttgtc aattgcttta taaaaaacct tgatggcggc tggttccttt ttgaaaattt
    87601 tatggtattc ggcaacgttg ttagctactt catcacagtc tatgtttatt tcttcgtttg
    87661 cattggcgtt gaatgccaga ccaaccatta ccgacatcat cgccgctttg attaaccgct
    87721 tcataatcca ccccgcttaa ttcttgtctt taagtgcttt caacatttct tcgatgaagt
    87781 cagcatagcg ctcatgttcg ttgggacggt agtttacgtt tttcattttc ttatcctctt
    87841 ttgcttcctc cggtttatcc ggcctttaaa tcccttttct gcttcctttg acttctttat
    87901 acaaaatgta ttcgttgaag tgaacacatt ttgtataaaa tagagacgca gatcgcactt
    87961 tttatgaggt taaatcatgg ctaccagttt aggtacaatt tattacgaag ttgatgcaaa
    88021 aactggtcag cttcttgtcg cgcaacgaca ggcggaccag gcttttgaca gtatagagcg
    88081 tggcgcaaaa cgcgctgacc gccaggtaaa cacgctgaag acatcaatca aagccctgac
    88141 cagggttata catctgctac ttgctgcgga ggcggttcgc cagtttattg acatggctga
    88201 gcaagcaaaa atgcttcgcg taaaaatcaa actccttaca ggtgaagcag aggcaacgga
    88261 acgggttttc gacagactga aagaaatatc aaaagaaaca ggccaaagcc tcaaggatac
    88321 tggcgacctg tggcaagggc ttgctatatc gctaaaaaac acatccgcca cagaaggcca
    88381 ggtacttaac ctggttagca cgctgcaaaa acttggtaac ttgggcggcg tgtctgcgga
    88441 acaattatca aactccatgc gccagttccg ccaggcgatt gatgctggcg tgttgcaagc
    88501 ggaagaattt aactcaatac gtgacaacac cccaacaatc atacaggaaa tggcccgcca
    88561 aatggggttg tcaatgggtc agttccgcgc tgaaatgctg gacggtaaga tcacggctga
    88621 aaggatgctc aacgcgatcc aggccgctac gcaggaaaca aacgagaagt ttgcacagtt
    88681 gccgcgcaca tccggcatgg ccttcaacga gcttaaagtt gaggttatgg ggcttgtcga
    88741 acagcttgat gatcttttcg gcatatctga cggcgttgta acagcaatag acttaatcac
    88801 tggtggcgtt gagggattag gtaaaggcgc aaaattcgcc gcaacatgtt tcaatacact
    88861 taaaaccgct ggtagcgagt tcatcgacat gtttgatgat gtggctgtta aggctggcga
    88921 ggtggcggaa gagattatca ggatggtaac gccaatcaag gcgctaatgg atggctacaa
    88981 atggatgaag gagatcattg ataaacgcaa ggaagagcta aacagcaata acgagaagaa
    89041 atttggcccc accattggca aattcatgac gtttaagaat gacatagaaa aagcaactgc
    89101 cgcttatgat gagttcatgc agaaacaggg tgaggctgac gatggcaaga tcaccggatt
    89161 cgatcagcca gttggtaagc cgaaaaaggg gaaaaaggga aaaaaagaca agaaatctga
    89221 agccgatcgg cttggtgatg aaggtataaa agtatcggac cagtacaaca aagacgccgc
    89281 cgctatgcgt aaagcactgg agaacggcaa ggccattgat gccgcattcg ctcagggcaa
    89341 aataaccctc cttgagtaca aggccgcgca aaaagggata ggcaaggaac tgaaggacga
    89401 attggcacaa atcccggtgg aagaattgca agacaaatgg gcgcacatag taagcccaat
    89461 ggatcagctt aagggcgaaa ttgacccaat caaacaggcg cagaatgaat gggcagtaag
    89521 aaagcaaatg ttattagacc tgggcgctac agaggcgcaa cagagacagg cgcagttaga
    89581 atatgaacaa cagatccgcg atctgaagtg ggagcagtgg caggcgcaaa gcgacacaaa
    89641 cggcctgatc ggtgcttgtg ttagcggcct caaaggtggc atgggtaatg ctcttaccgg
    89701 cctgctaaac ggtactcaat cactaagcga ggtttttgca aacctgggaa gcagcatact
    89761 taacaccgtt ggcaacaaac tatcagaaat tgccgccaac tggatagcag atcaattgat
    89821 gatggaaacg caaagcaagg ccacccaggc aagtacaacg gctggcgctg ttgcggccca
    89881 gggtgagatc gctgcggcgg cggccccggc agcggctgca actgctgcgt caactggtgg
    89941 tagctgggcg gcggctggtg ctgcggcgct ttctgcgatc atgtcgctgg caacgtcaat
    90001 ctttggtggc gggcgttata atggtggttc tgtgaatggt ggtagtctat atcgcgtagg
    90061 ggaacacgga ataccggagc tattccagac gagcaacggg cgtcagtata tgatccctgg
    90121 ggaaaatggc agggttattc ctggccgcga tcttttcagt ggtggcggca ttaatatgcc
    90181 tgttaacatc actgttcaga ccacaaacgg atttagcgac gaggacagtc gcagacttga
    90241 gcagacaatg gaacgtgtgg cgatgaaaat gatggcaagg gaatcgcaac gaccaggagg
    90301 aatgttgcaa cctcgccgca aataaactaa accccggtac aatgccgggg ttatctttat
    90361 tcaatttcat catttttaaa ttcaccgttt acataaagct gtaatgctct tattagttct
    90421 tcagcttgct ttttgtctat tacaatcatc tcgcagtcgc taacgatcca taatctcccg
    90481 tcatcttcaa tactaagatt aatgcttctg cattcttcag tctcatgaat aatcattata
    90541 aaccttcatc atgttattga tttcgtgaat aaatttactc aaaacttgta tatcctctct
    90601 caattttccc tccgtgtaca cttcttgcaa acaccgcgtc agtatacctg aaaaatggac
    90661 atgtatatgt atcccctttg ctatccatat attttagcac ccatgccatg cgttttacgt
    90721 tgcttttgtt tgccgggttt ttcattttat tttcctccgc aaatcacaat cttccgtttc
    90781 ttgctatata gctgcgagca aacatcacct gccagatagt ccagatctcc atcaacgtaa
    90841 atttatcgtc ttccataccc gcctcactta tggcaaccac gcacgccaga taattcatta
    90901 aatttatgat gctcgacgat cgcccatgtt ttcagatcct ggctatagac gcgccagtct
    90961 aaattggcgc ggaaggcgta gccgtaaccg tcctttaatt ttttgggtgc aacatccttg
    91021 tttaaaaacg cgttcagaag ttcttttgct tttttgatta cgtgtcccgg tgcgtttttc
    91081 tgtgctttca ggtttttgtc aatcgccacc aatttcataa tccaccccca gtcacattag
    91141 ttgaccactt tcaggaagaa atcacggtat tcatcttctt ttgcattcat catgaactga
    91201 ccgtatttga atgcgtcatc aaagcctttg actatcgcca ctcccacctg ttcgaatgcg
    91261 ttgttcagca ttaccactac atagcgttcc atattgtcac cctttgttgg tactgctttc
    91321 tcttcttgct ttctatatac atcttgttac gtcctgcgtg aagccatttt gtaaactggg
    91381 tataattctt gtgacgaagt tctcattttt cgcgtagaag cgagtaaaga tgtatgcaat
    91441 atgtaactta tagggggggg ggatcatgcc ggaagttttc agatggacgc cgcaaagaag
    91501 ctacagcgtg accagggaac caaatgtgtc ggtcgttaaa cttggtgatg gttatgaaca
    91561 gcgccaggag aaagggatta acacgttgct tgatagctac acccttgttt ttaaaggcag
    91621 tagcgcagga tgcggtgata gtgggaacgt agcgatccag gcagaagcat tcttgagggc
    91681 gcgcggcgct gtcgaggctt tttactggtc gccgtctatg gatggtgtgc aaaggctttt
    91741 tgtttgccgt agctggagta tgacaaagga cgggcctgta tacacgctaa acgcaacatt
    91801 tgagcaagtt gtcagatagt gggggttaat atgtacggga cttgtgtagt ggacaaaact
    91861 gacgctttta cattatttga cgactacgag atcaacgacc ttactgtaaa ggctgacaat
    91921 ggcgatatat ggtatttaca cgacgttggc gatgggtgcg tggggtgcag atccagggaa
    91981 gggaaggaag ttttattttt ggttgatgat gtataaaaac aaccccgctt cgtgcggggt
    92041 ttttcttgtc agtagttaat gtacatcaaa ctcattgggt gagcgtgcgc catattattg
    92101 gctatcgccg cccagtccca atcagcagcg tgaacaccaa atacgatcaa gcccattact
    92161 attagtgcta aaacctcgat tttcatgatt caccccatta actcataaag gcataaacga
    92221 tggcaatcgc gccagccact accaaaacat tttcgatcac ttcatccata atcgccgcct
    92281 ataagttatc acgcagataa ttgacgcctt tatcagtgac gaatgaatga ttaacctggt
    92341 tttcatccgt catgatgatg aataactttt cctgtaggta tttcgctttt gggtacagag
    92401 ttaaacagac ctggtacagt atcccgcgct caatcagcaa atcaataaat tcgtgttcat
    92461 ggtagccgat gatgcgagcg gcctgtttca acgtgtacac ataatcaccg tgattgcgcc
    92521 gccccatgtt ctttatagta ccctcctatt tcgtgcgctt cttttgaggc gttacacgca
    92581 tcaaggtagt tattaaaaca ccctatatgc cgatacaccc caccaatcat cactcttaca
    92641 atccattttt tattcgtttc attccaccat acgccaacgc acccgctctt atttcttcta
    92701 gataacggca tatttttcat gttttcttgt tttgttatca ttcttagatt ttctattctg
    92761 ttatcactcc ttatatggtt tatatggtca atcacatacc cttctgggat cgggccattg
    92821 tgcatttccc aaattatgtt gtgtgcctta tgctttttat tttgcacaca gataacaata
    92881 tacccgttgt gcaaacttcc ggcttccttg ccgttggtaa ttctaccgct tgttggtatc
    92941 ttccagaata atttaccttc attataagta aagatgtcgt gccatttcat aatttttaat
    93001 caaggcaact taaattatct atgcagaagt ctttcgcagc tttttcgtat tcgcgttttg
    93061 cttctggatc gtcagccgga aaaccttttg cgtgtatttc accagggcaa gactgatcca
    93121 tagaatcagc aaaatcaaca ttgacgttaa agttaatatc tttcgtgttc atctcttcaa
    93181 ccttatccgg tacttgtaca ggagtaatat caaaatcacg agcgttagac caacagcgaa
    93241 gtaattcgcg accttcatca cgcgccgcag catatgtatc aaataggccc atgcgttccc
    93301 attcacattt atcagtccaa acgtctaatc tgtatttttg cattttgtta acctctcatt
    93361 tgacaggttc tatcctgtac cccaaaacgc ggtcatcctc agcaagtaaa agcgcgtcag
    93421 taagtgcctc gccttcatct ttatacaggg ccacagtcat ttccctgcca tcagccagga
    93481 acaccgtcaa cctccacacc ttgtcagcca tgtcacacct ccgcgccatt ttgttaacca
    93541 tgcttccttt tgagtacaat atacatattg taaaaaacgc gatcaaccgt tttgatatga
    93601 tttagcgtga cgtcgatcac aaaatgacaa ggtgaaaaaa tgcgcagtat acctacagaa
    93661 atgattattg attccgtgga tgccggagtc ggcgcggtaa ttgacttgtt tgaattagac
    93721 ctcacgcccc tgggtggcga ggttatccgc ttccattccg gcgcgaatgg ctattacggc
    93781 ccggttatct ggaagggagt ggcttacaac agctacccaa tcgaggcgac tggcttcgaa
    93841 atgaaaaacg aaggcgttta ctcacgcccc caaatggtag tagccaacat tggcgggcta
    93901 atcactggga tgaataacga ttttaacgac cttcgaggaa tgaaggttac gcgccgccag
    93961 gtggaagtaa aatacctgga cgctatcaac ttccccaacg gcaacccaga tgcagatcca
    94021 tctattgagg ctgtatcttt ttacgtcgtt gaggcgatga gcgaagaaac agccgaccag
    94081 gtgcaatatg aactgtcaac gccaattgat gctgataaag ctgttatccc tgggcgcaca
    94141 attctcgctg acgtttgcca gtggcagtat cgcggcgacg gttgcggata tagtggtgga
    94201 cctgtagcta ccgataaaga cgaatcgacc agcgatccaa agctggacag atgcagccac
    94261 cgcttgagcg gttgtcgttt acgtttcccg cgtccaaatc cgttaccaat ttcctgtttc
    94321 cctggttcca gtaaggtagg ttaatcatgg taatggaaga caaaatgttg cgttatgccg
    94381 ccgcgcatcc gtttgaagaa gtttgcgggc tggtgataga taacgattat ttttacccgt
    94441 gcgctaacgt gtctgaaacg ccgtacaaca gctttaaaat ctcgcctgac gattatatca
    94501 aagctgacga attaggcgtt ataacggcag tttttcactc acatactgac aattcattgg
    94561 tattgtcagc acgggatcga caacagcaag ttatttccgg cctgccgtgg tttttgtgtt
    94621 ccggcggcag ggtaagaaag ttccgccctg tagctcacct gttaggccgt aaatttgagc
    94681 acgggaaaac agactgttac tcgcttttcc gtgacgccta tcacctttgc ggcgtggatc
    94741 tgcctgactt cgagcgcctt gatgggtggt ggctgcgtgg ggaaaacctg tatttaaaaa
    94801 acttgccgct aaacgggttt tatcaggttg acgcgcaaag catccagcca ggtgacgtaa
    94861 taatcaggca gccgttcaaa ggcgctgacc catgccacgc aatgatttac ctgggcgata
    94921 acactgtttt gcatcatgac aatgccggac tgttaagccg ccgcgagcaa atgcggcccg
    94981 cttatgttag gcaaactaat tcaatatgga gatctgacaa atgctcaaat ttagatttac
    95041 gggcaatctt cgaagatatt acggcaaggt gtgtttaaac gttgatacgc cagcgcaagg
    95101 acttcgctta ttgaccgcac agaatcagga gttcaagaag gcttttttaa acaccccatt
    95161 gcgcttgcga attgccggaa aagattatga cgaaaagacc gcgcccgcag cggttaatag
    95221 taaataccct gactggacta ccgtcattgt tgcgccagtg gtggaaggcg gtattgcggg
    95281 gattggcgtt gtaggctgga ttttgatcgg tgtttcggtt gttagcgttg cgttctcgat
    95341 ctttatgtct cgcaacatga agataaaaac atcagcagaa agcgcacaag ataacaccat
    95401 atctaacaac acctacacca gcgttgaaaa caaggtgggc cagggtaggc cagtgccgat
    95461 cctgttgggt gaaatgaaaa taggttcaaa tgtcgggtcg ttaggcatag acacaagcaa
    95521 caacagagac gctctagacg ttgtaagtta acaggagaaa agccatgagt agcggcggcg
    95581 gcaaagccaa aacaccaaca ttattaaacg ataacctgta tcataaacag ttttatcgtg
    95641 ttttagacat tctcagcgag gggccgatat acggtccggt aaaccagaat gcgccattaa
    95701 acaacgtaat gcttaatgac acgcccatca ctgacgcaaa cggaggtaca tcagtacccg
    95761 gtgttagcgt ggcgtggcgc aatggtacgg ctgaccagtc accaatcaac ggcttcaacg
    95821 ccattgaatc aacagttatc gtcaacgcaa aagtaactca cgatacacca ataataagga
    95881 ccgtttcaga tccaaacgtt aaccgcgtaa ggcttaatct tggcgttgat tctctggttc
    95941 agtcagatga taagggaaat caatacaata catccgttgt attgatggtt gatgttaaac
    96001 cttcatcatc atctacatgg tcacttgtta aagatattac cattggccca ggtaagcaaa
    96061 gcggcgagta cctcgaagcg cacatcatca aagcgccgga tgaaaaaccg tttgatattc
    96121 gcgttcgtcg cgtaactcca gacagcactg gcgatcttct tcacaatgat accaggtgga
    96181 gcagttacag cgagataatc gacgataatt tatcttatcc tcacaccgct gtagcaggcg
    96241 cggtaattga ccacgatcag tatactgata ctccgtcacg cacctatcac atgcgtgggc
    96301 tgattgttga tgttcctgat aactacgacc cggaaacgcg cacgtattca ggcttatggc
    96361 ttggtggctt taaaaaagcg tataccaaca acccagcttg gcttttccgg tatctggtta
    96421 aaaatgaacg cttcgggctt gcccgccacg ctggttacat tgacgttgat gacggcgcat
    96481 tatatacgct ttctcaatac tgcgaccagt tggtaaacga cggttacggc ggctttgaac
    96541 ctcgcatgac gcttaatgct tacatcactg agcaaatgag cgcccgcgac ctacttgata
    96601 acattgcagg tatgttccgt ggtatcgcgt tatgggacgg gcagcgcctt accgtgatga
    96661 ttgatgcacc gcaggatcca attgccacca tcacaaatgc taacgtcgtt gatggcgcgt
    96721 ttactcgttc aagtatcgcc cgcgcagaat cttacaacgc cgtgatcgtg tcatggactg
    96781 acccggaaaa cggttgggaa caatcaaaag aatacgtggc agatgatgaa ctgatcgccc
    96841 gcgatggtta taacgaaacc acgttagagg cttttggttg cacgtcacgc gggcaggcgt
    96901 accgcgctgg caaatggctg atagaaacag caaaacgcga accatcaaaa ttcacgttta
    96961 aaatggcccg tgacgccatt cactttacac caggggacat catcgagatc ctcgataata
    97021 accgcgctgg cgctcgccta ggcggtcgta tcgtggcgaa caatggcaaa gtgataacgg
    97081 tcgacaaggt tgattctgaa tatatcgcgg ctggtgacac catcagctta ctggatagcg
    97141 acggcaagtt taaaaaacac cagatcaccg gagtttacgg aaacaacatc acacttgcag
    97201 ccgcgcccgc atggattcgc aacggaaccg tatttgccgt gtcgactgaa tctgcaaaac
    97261 ctgttctgtg tcgaatcacc agcgtagcag aaactgaaaa taacagcgtg tacaccatcg
    97321 aggcggcaca gcatgacccg cacaaacagg ccgtagtcga tgaaggcgca atctttgagg
    97381 taaacaatga ttcgcttaat cacttccgtg ttcctaacat tgaaaatctg aaggtgttaa
    97441 acgttggttc tgaaacggtt caatgccgcg caacatggga aacacagaca acaacgcatc
    97501 gcctgacctt tgaaatccgc gtatataacg cagacggtcg cgtggtggta agctatgaaa
    97561 ccacgaatta ccgctatgaa ttttatggcc tcgatgcagg aagctacacg cttggtattc
    97621 gcgggcgtaa tgactctggc atgaagggcg ctgaaagtat tgtggatctg gttattggtg
    97681 caccagcggc ccctatcggc gttaattggg ttcccggtgt tttccaggca acagtgtacc
    97741 caatcagcaa aacaactctg accagcgata caagctatga attctatttt gctggcgaaa
    97801 cgcaaatcac gaatccggca ttaatcacca ctaatgcaca atataccgga cgtggttatc
    97861 agtggacgtt cggcggcatg aaaacagatc atgtatatta cgtttatgtg cgcacgcgca
    97921 atgcttttgg cgtgtctgat tttgtcgagg catcagggaa gccaacagat aacttcgatg
    97981 aaattacaga cttcgtgaca aaagatgtta tgaatagcca gcaattcaag gatttgatta
    98041 gcgacacaag cgcaattaaa gatgatgttg ataatctgaa gggggccacc tcagatttaa
    98101 ttaaccgcgt ccagaacaat gaggaagatt taggagtcca taaaagccgc attgatgaaa
    98161 tgggcgttga gatttcagag caggatagcg aacttgcaca agcgcgcgca cgcctgcaaa
    98221 acgtttctat tgcgcttctt aataactctg ttgcacaaac aacatctggg cgcacactat
    98281 ccgctcaact aaaacagtca agagcagaga tcacgcgtat agatgaggta gtcgcaacag
    98341 aaaaagaggc aacagcaaaa gcgctgacaa ctcttgaagc aaaaataaat gatgagatta
    98401 aaggatctgt caccaacctt gaacagacaa tggcaacact cgaaaaaaca acagcacaac
    98461 aaatcagcga ggtaacagcg tcaattaacg ggcagtccgt gcttatcgag gaaaacaaaa
    98521 cggcaattgc tggcatcagt ggtgacatgt cagcatcatg gagcatcaaa gttgcagcag
    98581 ataacaacgg catgaaatac gctacaggta tgtcgtttgg acttactggt gacggcacta
    98641 acgttcaatc gcagtgtatt ttccttgttg accgcttcgt attaatgact gcggcgaacg
    98701 gtacatatac aacacctttc tatgttgcaa atggggcaat gtatgtgcgt gaggcgttta
    98761 ttaaggacgc atcaatcact aacgcaaaaa taggttcata catccaatca aataactatg
    98821 tttccggtag cacgggatgg aaactagata aaggtggcac atttgaaaac tacggcgcag
    98881 ttagtggtga aggcgcaatg aaaatgacaa acacaactat ttctgttaaa gacggaaacg
    98941 gagttttgcg cgtccagatt gggagaatta ccggatcatg gtaaaattaa ggggccgact
    99001 tgttcggcct cagttttatt aaggaggtgc agttatggct actttcggcg tggcgacatg
    99061 ggacgctaac ggcaatccca acaactacgg aatcaaacca tccgtagtgg ttggttacat
    99121 ctccctttct tccggtcagg tgtcaggcac atatacattt acagttcctg cgggatgtaa
    99181 attatcttac tgcatgacgc ttgattacgg aacaatgtca acaacgcgca ggagtgttac
    99241 tgtttctggt ggtactgtat ctatttcatc agcaggggat acagttggcg ccaacatatg
    99301 gccagcatca cagtgtgaaa ttgtaatatt tgcggaggtg gcttaatggc aacttttggc
    99361 gcttatttaa gattaagtaa cggtaacatc tttgtcacac cagaagcaac gccactttgc
    99421 ttatatagac gtgtgacatt gacatcatct taccaaaccg tagcagttcc atctaataaa
    99481 cctgtgatgg tatttgtgaa aagccataca gcatcgcctg tattagctta tagagatacc
    99541 tcatcaactg taatgatcgg ggctggcaat ggctatgcgt atatttttac aatattcacg
    99601 ccgtcattgc cgaagtgggg gatggctatc tggaacgctt caggggagct agtgataact
    99661 aatgaatcaa gaatattaac agatcttgta acagttggtg ttgcaggatc cagcggtggg
    99721 atttatatag accaaacgtt atccggttct tacgccattt gcccgcaatt attaggtgca
    99781 caagttggtc agggcgccgc atcaatttac gcggcagcct attataacgg agtaaataca
    99841 agaatttacg gaataaaggt gggtggcgta tatgcaccaa caggatttat taatcatggt
    99901 aacgccatca ccgcaatcaa aacagacgca tacgattaat ttagttcagg gcaatccgtt
    99961 ttcttgaagt ggtcaacgct tacccactgg aagttaaacg gataacctgc cttgatcatc
   100021 ttatgcccgt caacatcagt gacgccgaaa accgcaacag cataatcatt gccgccagtt
   100081 tgataaattg ccgtgcattc acgtttaggc atatcagcgc agccaaacag acaagcaaca
   100141 attgcagcta tagcgatatt ttttaggcat ttcattgtct taccctcctt tgtttcgttg
   100201 tttgtagtat caacaaaatg tattttctaa acattgacat aaatcaacaa atacattttg
   100261 tgtaagcgaa aaatgcaaca cagatcacaa aatggtagaa ttattccgtt aattaacata
   100321 atggagtcat tgcgatgatt tatacgactg gaaccattgc catcaatggc aacaccgtta
   100381 caggtacggg aacagatttt tccgctccgc tttctctcat ccgtgtaggt tgcacgctta
   100441 ttgcagtcgg caatccggtg cagatcttta ccatcacaga aattaaaagc ggtactgaac
   100501 tgtcagtaac gccagcggct aatcctgcta tccctgcggg aacaaaattt agtattctgc
   100561 tttctgattc gatctcagtt gatgggcttg ctcatgacgt agccgaaacg ctgcgttact
   100621 accagggaaa agaaacagag attgccactg ccgttgagtg gtggaatgag ttcggcgggg
   100681 atggtcagat ggatcagctt cttgctgata ttcgctcaga gacagcgcaa tcaaccgcca
   100741 acgctcaaaa aacagaatca gataaaaacg cggcagcagc atcaaaaaca gaggcagcaa
   100801 actcagccac agcagcaaaa gcgtcacagg atgcagcaaa agccagcgaa acagcagcaa
   100861 gcaacagtaa aacagctgca tctaccagcg aaaccaatgc ggcaaaatca gcagccgatg
   100921 cgcttaacta tcgcaaccag gcgcagggta ttgtaggaac cagtatcgga cttggttcat
   100981 ctcctcgtga ttgtcctgac atttccggcg atccatccgg ttatatcgga tttatgcgaa
   101041 tcatggaagg cgcaacgggc tttccttcca ttgcttcagg agatggtaat ttaaccggct
   101101 ttataagcca ggttgacgaa tctcctgctt atacaggtat tttccagggc tggaacacca
   101161 gatctattta tacatatcgt tggtttagcg gattaggccc gcaatggtca caacacgcaa
   101221 gaaaagatga ggtaagcaga ttaacccagg gttctggcga atcagcttta tgggccgcag
   101281 gagggaagca aaaatttgtt ctgttggatg gaggggatgg gaatactcta tcttggggtg
   101341 tttgggataa tgttgctagt aaatggagtc cgttatcaat agcgcagggc ggtactggtg
   101401 cgattgatgc tctaactgct aggaacaatc taggggtggg tgtagaaaac agtcctcaat
   101461 tcagggatat gattctatca cacacaacag atgaatcatc tggctgggta tctggtggtg
   101521 tgcaaaaatt agtacttttg ggatctgaag gtactaaacg tgcagaaggt ttagcgtatg
   101581 cagaatgcaa cccagatcaa ccaacacaac tcacgtttgc tatatatgga gttaacgaag
   101641 gttataatta tttatccttc aatacaatgg ggcagatttt agccgaaaca gctattttca
   101701 gatcaaacta cgctaatcca ctggttatta aaagcagcac accaacaatc tgcttcaatg
   101761 aagaaggacg ccctgacgga tcaccttttt acaacttaat ttttgatgat ggtaactggc
   101821 gcattcagaa aaacggtgat gggtataatg gtgaatatgt aataggttac aactatactc
   101881 ttgatgaaat ggagcttccc aacctgctta caaaggctat taaattaaac tcaaatgatc
   101941 acgaaaaatc attgttgatt agagtagaag atagcacaaa atttagcacg ttaataaaag
   102001 taaacggtaa caacacagga agggactcag tttttgagtt agattttctt aataaagaaa
   102061 cagctgaata cgataactac ggcttttact gccagattct cactgacaat tcaattcaat
   102121 ttcaggttaa cggcgctgta aattgcacaa ccttaaatca atcatctgac cgcgacctga
   102181 aagacaacat tcaggttata ggtgacgcta ccgaagcaat ccgtaaaatg agcgggtaca
   102241 cttataccct taaggaaaac ggcctgcctt acgctggtgt tatcgctcag gaggtcatgg
   102301 aggcgctgcc ggaggctgta ggatcgttta ctcattacgg taaatggtta caaggcccga
   102361 cagttgacgg aaacgagcta cgcgaagaaa ctcgttacct caacgttgac tatgcggcgg
   102421 tgactggttt acttgtccag gtggcacgcg aaacagataa tcgcgtcacc gaactggaag
   102481 aggaaaacgc cagcctgcga gataacattg ccgcaatgga tgaaaggatc gcaaaacttg
   102541 aagcgcttgt cagtcagttg acaggaagcg aaaaataaca ggtgatgtgc gaagaacatc
   102601 gccgcgcgaa acgaggctaa cgcctcgttt ctttttgcgt accaaactgt gaccaaatat
   102661 caaaaaacaa aagcaaaagc actgtaacat actgttttat aaatgattat acacagcgct
   102721 aaccattcca gacaggacta gtgagtaagt ccagggtatt gaagttgtgc gtaatcattg
   102781 agatcccatt atttttatca atttttgttg ctatccgatc gcaaaaaagc acgtcatatg
   102841 atcagataat tctgataatg atagacgcta tttaacactt cacacggttt gtatacggaa
   102901 aagcattttg ctttttgtat tcaatttaga cagaatttta ttaatcattt cagggtaatg
   102961 gggtgatgag atgttgcgta acagggccag aaggctagac tacaaaataa tgcgttgatg
   103021 atggaggcac tgtggaagcg attaagggat cggacgttaa tgtcccggat gcagtatttg
   103081 cctggatgct ggatggtaga ggcggcgtta agccgctgga aaatacagat gtgattgatg
   103141 aagcgcatcc ctgctggctc caccttaatt atgtacacca tgatagcgcc caatggctgg
   103201 cgacaacacc gctgcttccc aataacgtac gtgatgcgct ggcgggcgag agcacacggc
   103261 cccgagtcag ccgtctcggt gaaggcacgc tgattacatt gcgctgtata aacggcagca
   103321 ccgatgaacg ccccgatcaa ctggtcgcca tgcgtgtata tatggacggg cggttaattg
   103381 tttcgacccg acaacgcaaa gtgctggcgc tggacgatgt ggtgagcgat ctggaagagg
   103441 gcacgggtcc gaccgattgc ggggggtggc tggttgatgt gtgcgatgcg ttgaccgatc
   103501 attccagcga atttatcgag cagctgcacg ataaaattat cgaccttgaa gataatctcc
   103561 ttgatcagca aattccgccg cgtggattcc tggctctgct gcgcaaacaa ttaattgtga
   103621 tgcgtcgcta tatggcaccg caacgtgatg tttatgctcg tcttgccagt gaacgtttgc
   103681 cgtggatgag cgatgaccaa cgccgtcgga tgcaggatat tgccgatcgc cttgggcgcg
   103741 gccttgacga aatcgacgcc tgtatagcac ggactggtgt gatggcggat gaaatcgctc
   103801 aggtgatgca ggaaaattta gctcgtcgta cctatacaat gtcattgatg gcaatggtct
   103861 ttttacccag tacctttctg acagggttat ttggcgtcaa ccttggtggg atccctggcg
   103921 gcgggtggca attcggattt tcaatttttt gtattctgtt agttgttctt attggtggtg
   103981 ttgctttatg gttgcatcgt agtaaatggt tgtaacaaaa gcaatttttc cggctgtctg
   104041 tatacaaaaa cgccgcaaag tttgagcgaa gtcaataaac tctctaccca ttcagggcaa
   104101 tatctctctt gcaggtgaat gcaacgtcaa gcgatgggcg ttgcgctcca tattgtctta
   104161 cttccttttt tgaattactg catagcacaa ttgattcgta cgacgccgac tttgatgagt
   104221 cggctttttt ttgcctgtta tttatcagcg tctacccttt aagagtccac ccaatgaccg
   104281 gagggaaata tgacgacact tatttatttg caaattcctg tccctgaacc gattcctggc
   104341 gatcctgttc cagtgcccga tccgatccct cgcccgcaac ccatgcctga cccaccaccc
   104401 gatgaagaac cgattaaatt gtcgcatcgt gagcgtagat ctgcgaggat acgcgcctgc
   104461 taactttgcg tcgatgacca cgagaataga ttgtgaccgc tttttctacc ctgaatgttt
   104521 tgcctcccgc ccaactcacg aaccttaatg agttgggtta tttaaccatg acgccggtgc
   104581 aggccgccgc gcttccggcg atccttgccg gaaaagatgt tcgcgtgcag gcgaaaaccg
   104641 gcagcggcaa aacggcggct tttggcctcg gcttgttaca gcaaattgat gcgtcgctat
   104701 ttcaaaccca ggctttagtg ctgtgtccta cgcgtgaact ggcggatcag gtggcaggtg
   104761 aattgcgtcg gctggcgcgt tttctgccaa ataccaaaat tttgacgttg tgcggtggtc
   104821 aaccgttcgg tatgcagcgt gattcgttgc aacatgcgcc gcatattatc gtggcaacgc
   104881 cgggccgttt gctggatcac ctgcaaaaag gcacggtatc actggatgcg ctgaatactt
   104941 tggtgatgga tgaggccgac cgcatgctgg atatgggatt tagcgatgcc attgatgatg
   105001 tcatccgttt tgcgccagca tctcgacaga cgcttctgtt ttcggcaacc tggccggaag
   105061 ccatcgctgc aatcagcgga cgagtgcaac gcgatccttt ggcgattgaa attgactcaa
   105121 cagatgcttt gccacccatt gaacaacaat tttatgagac atccagcaaa ggcaaaattc
   105181 ctctgttgca acggttatta agcttgcatc agccatcctc ttgcgtggtg ttttgcaata
   105241 ccaaaaaaga ttgccaggct gtctgcgacg cgctgaatga agtagggcaa agtgcattgt
   105301 cattacacgg cgatttggag caacgcgatc gcgatcagac cctggtacgt tttgctaacg
   105361 gtagcgcccg tgtactggtc gcgactgatg ttgctgcgcg tggtctggat attaaatcga
   105421 ttgagctggt ggtgaacttt gagctggcgt gggaccctga agttcatgta catcgcatcg
   105481 gtcgtacagc tcgtgcagga aatagcggtc tggcgatcag tttctgtgct ccggaagaag
   105541 cacagcgggc caatatcatt tctgacatgt tgcagataaa acttaactgg caaacgccgc
   105601 cagctaatag ttccattgcg acgctggaag cagaaatggc aacgttgtgt atcgatggcg
   105661 ggaaaaaagc caaaatgcgc ccgggtgatg tattaggtgc actgacagga gatatcgggc
   105721 ttgatggcgc cgatattggc aaaatcgccg tgcatccggc gcatgtctat gtcgcggtcc
   105781 gtcaggctgt tgctcataaa gcatggaaac agttacaggg cgggaagatt aaaggaaaaa
   105841 cgtgccgggt gcggttatta aaataatgaa atgttgaatt gccgggtgca agagtaaaca
   105901 tcttattcgg gattgccgga tgcgacgctg gccgcgtctt atccggcctc cataagagta
   105961 gcccgatacg cttgcgcatc gggcgctatc ctggctattt cacttcaacc acattcagcc
   106021 gtaactcatc caactgattt tcatcttctt ctggctgcca gcccgccggt tgtagtggga
   106081 tctcttcgcg atcaaacgcc agatcacccc cgttaaccac ttcagaaccg tgggtgatgc
   106141 ctttgaaatc gaacaggttg gtatcgcaca gatgcgacgg caccacattc tgcatcgcgc
   106201 tgaacatcgt ctcgatacgc cctggataac gtttatccca gtcacgcaac atgtcagcaa
   106261 tcacctgacg ttgcaggtta ggctgtgaac cgcacaggtt gcacggaata atcgggaacg
   106321 cttttgcatc ggcaaatcgc tgaatatctt tctcgcggca gtaggccagc ggacgaataa
   106381 cgatatgttt gccatcatcg ctcatcagtt tcggaggcat acctttcatc ttaccgccgt
   106441 agaacatatt taagaacaac gtttgcagga tatcgtcacg atggtggccc aacgcgatct
   106501 tcgtcgcccc cagttccgtt gcggtgcgat aaaggatacc gcgacgtagc cgagaacaca
   106561 gtgagcaagt ggttttgccc tctggaatct tctctttcac gataccgtaa gtgttctctt
   106621 caacaatctt gtactcaacg cccagctttt caagatactc gggcagaacg tgttccggga
   106681 agcccggttg cttttgatcg aggttaacag ccaccagcga aaaattgatt ggcgcgcttt
   106741 gctgcaaatt gcgcagaatc tccagcatgg tatagctgtc tttacccccg gagaggcaaa
   106801 ccatgatgcg atcgccttct tcaatcatat tgaagtcagc aatggcttcg cccacgttac
   106861 gacgcaggcg tttttgtaat ttgttcagat tgtattgttc tttcttcgta ttcttttgat
   106921 tttcttgcat tatttcggtt ctctggtact aaatggggca aattgggggc aaactttgca
   106981 actacgatag ccgcgcattc aacatggtta cctgttcgtc gttcatatca tcaatccaca
   107041 taccgtaaat ttcatacacc atctgcgcag tttcatgccc catctggctg gctataaatg
   107101 ccgggttcgc tcctgccgtc aacagccagc aggcaaaagt atgccgcgta tggtacggat
   107161 tacggcggcg aataccagca cgttttactg ctgcattcca ccttgccccc aaactgctta
   107221 ccgagtaata aggtttctgt tttccgttac acaccctggg catgaaaaca aaatgcagtt
   107281 tttgcttttc agttctgccg tactcccgat gataaaaagt gatttcgctt ttgcgatgat
   107341 gcccggtcag tttgtattgc tccttcagtg cttcaagagc cggctgcagt aatgttaccg
   107401 tccggatccc ggcatttgtt tttgggggac cgaacatatc aagtatcgtc aggtttcttc
   107461 tgacattcac aattcccttc tcgaaatcca catcctccca cgccagagct gccagttccc
   107521 cgtgacgaag cccggagtaa acggcaaatt tccacaagtt ttggctctgt cctttttcac
   107581 tttccattaa tgcattgaat tctgttttag ataacgggtc aggctttatt ctgtttcgct
   107641 gtaatttttt tactccttca aatggtttgg ttgatataaa tcccgactga tacgcaaaac
   107701 gtaacaacga acagagcagg gcgatatagt tatcaactgt gcgcacggtt cttccttttt
   107761 tattggatct tggattatcc aggtaaagcg tttctccatg caacagttca ttccggtaat
   107821 ttaagatatc gctatgacga atatgtgata tcggggtact ctcacaaatt attatcctga
   107881 gtgtttttaa ttgtgatttc gttttcttca ttgtgtttgt tgttaactct gtttctttaa
   107941 tttttgtcca gatatcacaa agctccccga atgtttttat gaccctcgtt gtcaccattt
   108001 ttgccccagt gctggactgg ggaaaacgtc ttaaatactc aaattcaccg gaactgattt
   108061 catgaactat caacgctctt aaattcccgg cctttttaat gttactgtta gtaatctccc
   108121 agcctttcaa tgtttcccga catcgtttgc ctcgaaacat gaaccagatg cgaatgtatt
   108181 tacctcgaat ctcgacacct gttggtaatt tagacatatc atgagtcttt gataaactga
   108241 tttatcttcg gatagttgta ccagataatc cctcgcttgc tgtctggctt acctaaagga
   108301 gatactcgtt tgaagtgaaa gccttccacc cagcagttct ggcggtatgc ttcaatttgt
   108361 ctggccccca gaccagtacg aagcatcaga ccgtattcaa ccatccactc ttcattaaag
   108421 atcacttgtg ccatcgcatc acctctggcg ggcgacaata ttagactgaa attgacgccc
   108481 gacgttgatt attaataatc agctatgaag ttttaatttg aatacaatgc aactcacgag
   108541 gacagaagtt tctcgcaatt aaaatttatc agttttactt tctgctctct ggaaacgcct
   108601 gcttcttttt tccctgagag cattttttcg cattctgatt tcgttaattt agattttgaa
   108661 tatcttgtcc agttagtagg tgtgccaccc tccttttcaa tagtggcggt aattttatac
   108721 atgaacacct ccattattat ttccagtaat tcgtttattc catcttgcaa gtgcttcttt
   108781 ttcacttcca ctatagccgg ttcgggattc gcacccgtta cacttcgctc ggtaataccc
   108841 tgaaatggct ttcactgtca cagatggaca accacaaaac ggacatggct taactttttc
   108901 atatcgcatt gtcttttctc tcataaatat ataattttgc gatggcggcg aggctacacc
   108961 gccatagtaa atatcaggag ccgatgtatt ctggtttcag gtctgtcagt gtcgttttat
   109021 acgcctcata taattctcct aaatgtggtc gtacagcatt cagcgtgttt tccagagccg
   109081 taaatttttg ttctgcttct ggatcacctg atgaaggcag gtcatttatc atcttttcaa
   109141 tacgggcaat agcattaaga cggtgatgac gctgaaccac ttttccttta agttctgtgt
   109201 agagagcacc aagtgtattt ttatgagctt ctacttcctg gcgaagtgcc gttgtttctt
   109261 cggtgctttg tgcctgctca atacgttcac ggaaagcatc gatccagttt tccccggcat
   109321 cctgctcaat aattgttgtt tcacgttccg cacagcaggc ggatgtgttt ttatgttcct
   109381 gaaccggatt aatgattttt tcctgtggtt cgtccagttc gtcctgggtg tacactccaa
   109441 gaatcacttc aggacaataa aggcgtgccc agcgtttcag tgccagatag gcaagttgct
   109501 ggcgaggatc atcggcccat aacgttgagt tacgtgttct ggcctgcgcc agaagtaact
   109561 caagtacgcg tggtttgctc tctccacgta gcgttgcctg gacacgaacg ccgatcgcgt
   109621 tttcatcggc cagcttccat ccaggtacgc gatattcttt ccctttgtcg ctcttcctga
   109681 tttcaaattt cccgataatt ttttcccacg gtccgaacca gtcatattca atacgcccgg
   109741 ttagcggccc acgagtactg attacggcat taaccagttg cgcttcatat ccgagcacac
   109801 cattcacaac gaaagttttc tgagctactg cgtaagggtt catttgccac tgcatcgcct
   109861 gcatggtgat ggccatacag tctgatggat ttccccggag gtgttccggt acagtagcca
   109921 tgccggaagc cattacctga gaaaatgtct gaattgcagc cagggactga gggctgaaaa
   109981 ccgcaacgtt agagttaata ttttcttgtt gagttaattc gttcattgtg tccttcctca
   110041 gatgctcagt gcttcaagac gacgaagatc aaagtcgttt aattcgtcgg tataactttc
   110101 ggtaattggt gctggccagt tgtttgtctc cagagcttcg tttatctggc gtagcgtccg
   110161 gcgatactcc tggcgaccaa gttcaaggag ttcctgcgaa gcttccacaa ctgccaccca
   110221 gtgatagcca gcatccttat tgacgaagac ccagaaaaat ttgtccaggt ttgccacatc
   110281 gcagtacatt gcggcgctca agtgataatc acgttcaata atttcacggt gtaagcgatc
   110341 ttttagtcgt tcttgccgca cataaccgag gcttactgat ttcacgtcgg cgcagatgct
   110401 ttcgtatggc agtcggattt cgatatcagg gcggaccctg atttccagcc cggtttcttc
   110461 atcaaacccg aaatagctga tttcagattt gcgatccggg tggttgagta gtcttgccgc
   110521 gtcagtattg ttttgcagtg ctgcgtgaat attttttgcc tgttcataca tatctggact
   110581 gataaacgtt ttcccggctt tttcttcttg ctggcgtttt tgccagtcct ccagtgttac
   110641 cagttccggg cgaattttcc gggcgatttc ggttaattgc tcttttgtgc cactgatgtt
   110701 gtaaggcaac gatttagcac gttctttttt tgccagttct ggatctacag tttcaatttg
   110761 ctccagaagc tgctcccgtg ttccactggt tttcagcaga ggagggaggc ttgcgttgta
   110821 ttctttaata caggctttca ttgctgatgc tgtgtgtttt tccccctcag gaatacgccg
   110881 aaattcctcc ggaagcgaac cgtaaaggat gcctgtttct tcggccccag cgcttaccga
   110941 cagtggctgt ataagagtgc tgttgtagct ttcgagccac tctttcatca gctctggtgt
   111001 catcagtgat ggcagactgg cattgtgttt tttaatgatg gcgatcagtt cgttagaagt
   111061 agtaaccaca tattcaggaa ccggtaccgg aatggtatat tcatcagcga atttatccgt
   111121 ttccagaaca tagctgtgaa tgatccgccc acgcagcaat gcatcacttt cctcgttcgg
   111181 aatagttccg gcaatgtgcc gcccgtggta atacatcaga ctgatgcggg catccttcag
   111241 cattgtgctg cttattccgt tggcggagtg ataaacctcg ttcgggaggt tttcatagcg
   111301 gccaggctcg aaatatgacg gccacatgat ttcagttgct acatgagctg acgcttcacc
   111361 agtttcatca ctgcaatcgt gatgcggatg gttgccagcg ttctccttgt gcggatgttc
   111421 agcgccttcc atttcctccg gatcattttc cttagcttca acctgattct cttcatcgaa
   111481 tgtttcctgg tatgttgcgt cgcccatcac cgcaccacag tcagggcagt tatccccgcc
   111541 agtctgaccg caggcattgc agactatttc ctgtttctgt tgcactactg actcaggttg
   111601 tttcacatcc gggctggttt ttacagtttc tggcgtgttt tgtttcgttt ctggctggtt
   111661 ctggtacaca gagtcgcgag tctggatccc ctttacccat ttcggatcgt tcgggtctct
   111721 aatcccttca acgaactcac cacgcgaggc agccagcaat ttgtcggcat cgacaggatt
   111781 ttttgatgga atgtttttcc gggcttcatg gagttctgcc cgcagttcct gatatttcgc
   111841 atcagcagaa tttacctgtg actgagcatc cagcggctgc gtgtcctgat gatgttcagt
   111901 tgcgtccggt tccattgttt cagcctctcc ctgttcatct gccgttgttc cagatggttg
   111961 cggtttttct tcatcatcct gttttccttc ttctgttact cgctgcggca tcggggcaga
   112021 ggagcgaccg caggcaatat ccacgatttc cggatcaggg ttggcatgat cggtttcagt
   112081 cagtactttg ttcagatatt cagtgacgtg cgcggggatg tcctcgatcc caattggtgc
   112141 ttctttcacg gacgcaacca cgatggcgcg agaataatcc agcccgccag gcatggtgat
   112201 gaatttgtcg cggaaaacag aaaagggcgg tttattttca gcgataattt ccttaatgcg
   112261 tttagcgtgt gccggatgaa ggttatagat gtccacgtcc attgaacggg ccagtacgcc
   112321 agtggctaca tcgcgcgtca gtgacgtcag atcgtggacg aaaccttcgc cgcgatcggt
   112381 gaggttcccg ccgccagcat tagcaccgga agccgtgcga gtgatgcgtg aaacatgatt
   112441 cccttttcgc cattcttttg tcagaagacc gcgatcaatg tgttcggtat ccagccaggc
   112501 tgaaatgaaa tccttaaatt catagggctg atgttttttc gtgatagaga aaactgcctt
   112561 aattgcatca gtcaggcgga gcagggcggc attatccaga gttgtcggtt ctgccatgcc
   112621 gcgtatggcc aacagcagat tctggatata gctgttttcc tggtccatct caagagcagt
   112681 aatgtgttcg cgttgttcac gggtggcatg atgcaggtat ttccgatccc cggccgcata
   112741 cgtaaaaatg tgcagaagac gctgtgtgaa ccgcaaagtg gctacagaga cttcgcaatc
   112801 ctggcaatcc ccgtgggcgt ctgcctgcgc attttcttcc tgacctcccg ccagttcttt
   112861 ggtttcctga gcattatcct gggggtgaac gtcgtctggc gctgctcccg gttttagttc
   112921 ccaggtcatg gagtctttgc tgagttgata gcgttcactc caggtaaaat cgatctcacc
   112981 ttcagggggc aggtcattca cgacaggaaa attcgtggca acagctttaa aatagttgct
   113041 cagtttttta cctgacttaa cgatcaggta gtcaagagtg gcacaggttg attcaaaatc
   113101 gtcgctttcc cacaggacga cgtcaggttc accggatgat tttttcgctt tccgtaaaag
   113161 gaagagtggt tttgtgctca ttgtttttta acctcaactc agattaaaat tactgcgagt
   113221 gatgaataaa tgtcccaggt tcttcactca ggcctgcaca ctgtgcaggc tttctttttt
   113281 tcagatttca ccgtttaatt tcattgcgat cagagttgcc agaaatccgg cttttttttg
   113341 cgggcagatt ctttccgatg tgaaccaggc acatttttgt gacaccttca tcaagtgttt
   113401 ttacgttgcc tgatggacca tcgatatcaa ccactgtgaa tggggtttct ttattttctg
   113461 tcttaattac gtagccaata cgctttcctt caagacttac ttcgtgaaca atgtcatcag
   113521 tagtaacagt agtgacttca taactggtaa tcatatgttt ctccttaatt aaggttgagt
   113581 gaatccctgc cattgctggc ataaattcag tttcgaatag tcagttaatt aaagttcgtg
   113641 tgccatctgg tctttttcgg cacaactttc actacaatat tttttcattt ccgtcgttgg
   113701 gataactcca cgcatgaaat gaagtggtct tttaatactt ttgctttctt caatttcttt
   113761 attgcaaagg tggtaagcac attttatttt cttagtcatc accatgactc cgcctttaca
   113821 ggtaaaccat cacgaccgag gaagacttta atcatgcagt cagtaatgca tgtttttgtg
   113881 gtcaggttac gaatataaag ttttcgcttt ttaatattgt ttgccgaggc aatatatgtc
   113941 cggccttcat gaagtacata gtcaccagga gtcacacact gacgtggtat ttcatcagtt
   114001 ccgaagtgat gtacaatcat aattatctcc atttttacaa atgaactttg tttatgcggt
   114061 gcctggtgcc tccaggtgac tgcaaccagt taacaattac agtcggcttt cccaccaaaa
   114121 ccaataagga ctaacatgac ttttaactgt gccgcgtgca cttagccgca ttcaccgcat
   114181 cacaaaattc actttaaaaa gggcggacat cagtcgaact tcaagaaaaa actgatgccg
   114241 ccaagactac acacagcagt gttgttattc acaaccggag gcgcactccc accatttaaa
   114301 tttaacagac aagaccgact ctttatggat atcggaaatg cgccttcgtg ttgcgcccgg
   114361 ttttatttca ccacctccgg gctttggtgg tctctgctat acccctacag cgagaatatt
   114421 gaataaatcc aataaatgga ttagctggta tttttgataa gccagcgacg cgcgccagct
   114481 tcagttttaa acgttttgct tttggtatac gtcatcgcga tgaacgtgcc gttctggttg
   114541 ggaaacacgc cgtatacaag agattcgttg ttgccaagat cgatagtatc catgttgacc
   114601 tcatttcccc ttaacgctgg gtggcggaac gttttatcta ctgcgctttg tatcaatcaa
   114661 caactgccgt catgttcgta tgcctcgggc tggctactta acccctgacc actgccgggt
   114721 aactcgaagt attgcctggc gttctgtggg gtggggtagt atgcaataaa tctaaaataa
   114781 tttagttttt cagtcaaggg aaatctagat taattttaaa aaaaggccgg catgccgacc
   114841 tcaagttaag gacgtggcgg gttataagtt gaactgtaca ccttttgcta cggcaacaat
   114901 tttacactct ggtgtaagta aggctgactg atagcgtgga ttgagagggc ttaaatacac
   114961 aagcttccca tcaataacta atttctttat agtcatagat ggttcatttt taagcggatc
   115021 aggaactatt acagcgacga tgctgccatt tttataactt tctcctggcc ttaggatcac
   115081 agtagcacca actgggatac tcggtgaccc tgatgggtta tgcattgtgt catcaggcat
   115141 tgaaacggca aaatcacctt cctcgacatc aaagaatgtg gtgatcctat cgacatttcc
   115201 cattgttttc tctcctttta agattaggaa agaaatcgcc tcaccccacg aaaggtaggg
   115261 gatcttggtg cctgaggtgc tttgtacaaa agataattca ggagacgata ctccatatag
   115321 gagataggac tcagtagttc ctaatgcctg ggctaactta cttagagctt tgctacctgg
   115381 ttcgtttaga tctttctccc agtaccctat agtaacccca gtcacgcctg aaagcttacc
   115441 cagttctact tgggtcagcc ccttatcttt tctaagtctc ttaagcctga tgccaaggct
   115501 ttccatcatt ttctcctgca aattgaatat aaattatttt agattgcatt gacctaaaaa
   115561 aaattatctt gtaatctaaa aatacttata tttgggaggt agaatgcgag ttgatgaact
   115621 tgtccagttt tttggttctg ttcagagggt tgccgatttt tatgggataa cccgcgaagc
   115681 tatttacatg tggcgtaagc gtcctggtga aatagttccg aaggggaggg ctgcggaagc
   115741 tgctgcatac tccaggggga aattatcttt gaacccagaa ctttacaaaa agaaggatac
   115801 cagtcagggc gaaggaaagg gggattcatg aaaatcaaac atgagcacat ccgcatggcg
   115861 atgaatgcgt ggctgcttta tccgagggta gggcgcaaaa aaatcgctga tgatatagcg
   115921 acagcatatt ttgagcttga aatgacttat ccaccaatgc atgacacctc tacgacagag
   115981 ggtattggat tgaacataca aaatattttt cgctggcttg aaaaggatac gcctgatgct
   116041 gttgaaaaaa ttcaggcatt aattccggct atcctgactg ttcttccgcg tgaactgcgt
   116101 tatcacctca gtatttttga cactgttgag cgccgtgcat tactggcggc gcaggaagcg
   116161 ttgagtacgg caattgatgc acatgatgat gcagtccagg ccgtttaccg gaaagcgtat
   116221 ttcagcgacg gcgggtcatc cggcgagtct gttgtggtgc attgatattt atgccagacc
   116281 cctgctgatt ctgttgattg gggaatcaca gaatatcacc agaggatggt tcgtcacaag
   116341 atgaggcaat tatggccgca ttaccataca tgcaactgta catagctgat tacctggctg
   116401 acaccatgca tttgtcagca gaggagcatg gtgcgtattt gttgctgatg ttcaattact
   116461 ggcaaacagg aaagccaata cctaaaaaca ggctggcaaa aattgcccgt ctgactaacg
   116521 agcgatgggc tgatgttgaa ccatccttgc aggagttttt ttgcgataac ggcgaggaat
   116581 gggtgcatct tcggattgag gaagatctgg catcagtcag ggaaaaatta accaaaaaat
   116641 cagccgcagg aaaagcatct gttcaggcca gaagaagcag aaaggaagca gatgttcaaa
   116701 caaaacaaga gagaaattta acaggtgttc aaacagatgt tgaagtggtg tttgaacatg
   116761 atgtcaacac aaaggcaact aataaagata cagataaaga tctaaaaaca gatccccccc
   116821 taaatccccc ccgggggaat cgaggtgtca aaaagtttga ccctctggat attgctttgc
   116881 cgaactggat ttctgtctcg ctttggcgtg agtgggttga atttcgccag gcattgcgta
   116941 aaccgattcg aacggagcag ggcgctaacg gggcgatacg ggagctggaa aaattccgcc
   117001 agcagggttt ttcacctgag caggtgattc gacacagcat cgccaatgaa taccagggct
   117061 tgttcgcgcc gaaaggtgtt cgacctgaga cgttactccg acaggttaac accgtctcgt
   117121 taccggatag tgcgatcccg ccaggcttca ggggataatt gaccatgaaa aatattgcga
   117181 caggcgacgt tcttgaacgt atccgcagac tggccccgtc acatgtaatc gcgccattca
   117241 agacggtagc ggagtggcgc gagtggcaac tttccgaagg ccagaaacgt tgtgaggaga
   117301 tcaaccgtca gaatcgtcag ttgcgggtgg aaaaaattct gaatcgctct ggcatccagc
   117361 cattgcaccg caaatgctcg ttttcgaatt accaggtgca gaacgaaggg cagcgatacg
   117421 cgttgagtca ggcgaaatcc atcgctgatg aactgatgac cgggtgtaca aattttgcgt
   117481 tcagcggaaa acctggtacc gggaagaacc acttagcggc agctatcggg aatcgcctgc
   117541 tgaaagacgg tcagacagtg attgtggtta ccgtggctga tgttatgagt gccatgcacg
   117601 ccagctatga cgacgggcag tcaggcgaaa aatttttgcg ggaactgtgc gaagtggatc
   117661 tgctggttct tgatgaaatt ggcattcagc gcgagacgaa aaacgagcag gtggtactgc
   117721 accagattgt tgatcgccgg actgcgtcga tgcgcagcgt ggggatgctg acaaacctga
   117781 actatgaggc catgaaaaca ttgctcggcg agcggattat ggatcgcatg accatgaacg
   117841 gcgggcgatg ggtgaatttt aactgggaga gctggcgtcc gaatgtcgtc cagccaggaa
   117901 ttgcgaagta atttttaccg ggagaaaaat ttaatggaga ctgtttttga cgcactgaaa
   117961 gcaatgggaa aagccacatc catagaactt gctgcgcgac ttgatatcag tcgggaagaa
   118021 gtgctgaacg aactatggga actgaaaaag gctggttttg ttgataaaag cgcgtacacc
   118081 tggcgtgtgg ctgataacaa cgttcagcag gaacagccag cgcaggcaga actgccggaa
   118141 gaaaccacca cagcaacagt agcgaaaatc tcagagtgcg atttaaccgc gacgattgaa
   118201 caacgcggac cacaaacgtc tgatgagctg gctacattgt tcggtaccac atcacgcaaa
   118261 gtggcctcaa cgctggctat ggcaatcagc aaaggacgca tgattcgcgt aaatcagaac
   118321 ggtaaatttc gttactgcat accgggcgat aatttaccag cagagccgaa agctgcatcg
   118381 gtaacggaaa atgatggtaa agcctttcct cagccagcag gtgttgcgtt accagtacag
   118441 gaagctgcaa cacaggaaga tattaaaaca gaaactgtgg cggacattgt gcagtcgttg
   118501 ccatcgttca ccgaaacgca agcggatgac atggttttac catcgctgca tatggcaaac
   118561 cgcgaactgc gtcgggcgaa aagtcatgtc cagaagtggg agcgtgtctg cgccgcgctg
   118621 cgggagctga acaagcaccg ggatattgta cggcagatga attgttccgg agaggatgtg
   118681 aaatgagatg gcgtggctgg gtgtgggctg aaatccttat tctgcggcag tgcgcgggaa
   118741 caatgaaggt aaaaatcatc ggcagtctga ttggccgtag tgaggcggct gtcagaacga
   118801 aagcccggga gatgggaata agtctgattc tgcgtggtga ttttcaccag tcagcaaaat
   118861 atctgcagag agatattgag ctggtacgac aactgcatca gcgtggcgtg cccaggagag
   118921 aaatcgccag aaaattcgga attccgttgc gcacagtgaa taactacgtt tatttcgaca
   118981 ggagggtgtc tgcgtgaaaa tcctgtatca ggattacggc ccggtggggc aggtggttat
   119041 cagcagtact gtaatggagt tccggaagca taaccgtgtg gtggatgcag tgctgttatc
   119101 ctgtccgggg atatcggcga gccgtgcagg tgtgtttttt atgaagacga agttatatgg
   119161 cagtacagcg tggataaaga aggcgtatcg tgtagcgttg caggaggtta acagtgagtg
   119221 aaattaaaga gatgctggta gttcgtgacg aatatggctg ctggacgcat cctgaatatg
   119281 aaaaattttg tgatggtagg gaatatattt caacggaaga atttaacgcc tagatggagt
   119341 caaataatct tcagtggact atccggacta tggatgaaga tgattttaat ccggatgcag
   119401 atggtcctga tatttccgcc tgggagtcgg aacggccgga gggtgatgga tggtttattg
   119461 gttcgataca tgacaccgaa gatggcccgg tttgtatctg gttgcggaaa attgtgaggt
   119521 gacacagtga atcagattaa ctgtcaggca ctgcgtgagg cggcagaagc aataaaaata
   119581 gcagccacac cacaaaaatt gctggcattt cgtatgaaag ccacaccgca ggttgtgctg
   119641 gctttactgg atgagcagga agccagagac aaacgcatta cagagatgga agtaagagag
   119701 gtgcagttat cgacttgcta tgaacctcga tatggtcatc cgataaatac tgatgagcta
   119761 tgcgtcatga tacccaggga gcatggacgc tggatttatc tggctgactt agagcatgca
   119821 ctacgagtgg caggtattcg catcaaagga cagtgacgtg gaaataaccc cagaagatga
   119881 gtcctggaat attgttttat ttccggtaaa agatgatgac ccacgtaatc aggttaattt
   119941 tctttatgag ccatcggaga aaccatattg ccatcacgct tctgtcaggg ttgatgaaaa
   120001 agagcgtcag gtccgctgca aaatatgtgg cgcagttgtg gagccatttg actggatgct
   120061 ctctgtggcg aaaagagaaa ccagactggc agatgatgta aaacaattgc gtcaggagga
   120121 gcgggaaaga cgtaaaaata tagaaaagtt aattcagatt gagcgtaatg tgaaagcgcg
   120181 gatacgca
//
LOCUS       DAZFFG010000003       118004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFFG010000003 DAZFFG010000000
VERSION     DAZFFG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 118004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.003"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            12..596
                     /gene="yraH"
                     /locus_tag="ACHJ7J_000290"
     CDS             12..596
                     /gene="yraH"
                     /locus_tag="ACHJ7J_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4998574.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGV
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            676..1371
                     /gene="yraI"
                     /locus_tag="ACHJ7J_000291"
     CDS             676..1371
                     /gene="yraI"
                     /locus_tag="ACHJ7J_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4998575.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGLVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1400..3916
                     /gene="yraJ"
                     /locus_tag="ACHJ7J_000292"
     CDS             1400..3916
                     /gene="yraJ"
                     /locus_tag="ACHJ7J_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4998576.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3927..5018
                     /gene="yraK"
                     /locus_tag="ACHJ7J_000293"
     CDS             3927..5018
                     /gene="yraK"
                     /locus_tag="ACHJ7J_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4998577.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5061..5921)
                     /gene="rsmI"
                     /locus_tag="ACHJ7J_000294"
     CDS             complement(5061..5921)
                     /gene="rsmI"
                     /locus_tag="ACHJ7J_000294"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO4998578.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTHHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5986..8022
                     /gene="lpoA"
                     /locus_tag="ACHJ7J_000295"
     CDS             5986..8022
                     /gene="lpoA"
                     /locus_tag="ACHJ7J_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO4998579.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITLRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7980..8375
                     /gene="yraN"
                     /locus_tag="ACHJ7J_000296"
     CDS             7980..8375
                     /gene="yraN"
                     /locus_tag="ACHJ7J_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO4998580.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8395..8985
                     /gene="diaA"
                     /locus_tag="ACHJ7J_000297"
     CDS             8395..8985
                     /gene="diaA"
                     /locus_tag="ACHJ7J_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO4998581.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHHSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8995..9570
                     /gene="dolP"
                     /locus_tag="ACHJ7J_000298"
     CDS             8995..9570
                     /gene="dolP"
                     /locus_tag="ACHJ7J_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO4998582.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9684..10724)
                     /gene="yraQ"
                     /locus_tag="ACHJ7J_000299"
     CDS             complement(9684..10724)
                     /gene="yraQ"
                     /locus_tag="ACHJ7J_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4998583.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10797..11432)
                     /gene="yraR"
                     /locus_tag="ACHJ7J_000300"
     CDS             complement(10797..11432)
                     /gene="yraR"
                     /locus_tag="ACHJ7J_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4998584.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11560..12078
                     /gene="yhbO"
                     /locus_tag="ACHJ7J_000301"
     CDS             11560..12078
                     /gene="yhbO"
                     /locus_tag="ACHJ7J_000301"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO4998585.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12058..12501)
                     /gene="yhbP"
                     /locus_tag="ACHJ7J_000302"
     CDS             complement(12058..12501)
                     /gene="yhbP"
                     /locus_tag="ACHJ7J_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO4998586.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12552..12854
                     /gene="yhbQ"
                     /locus_tag="ACHJ7J_000303"
     CDS             12552..12854
                     /gene="yhbQ"
                     /locus_tag="ACHJ7J_000303"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO4998587.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12841..13344)
                     /gene="yhbS"
                     /locus_tag="ACHJ7J_000304"
     CDS             complement(12841..13344)
                     /gene="yhbS"
                     /locus_tag="ACHJ7J_000304"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4998588.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13338..13862)
                     /gene="ubiT"
                     /locus_tag="ACHJ7J_000305"
     CDS             complement(13338..13862)
                     /gene="ubiT"
                     /locus_tag="ACHJ7J_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO4998589.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14071..15066
                     /gene="ubiU"
                     /locus_tag="ACHJ7J_000306"
     CDS             14071..15066
                     /gene="ubiU"
                     /locus_tag="ACHJ7J_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO4998590.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15075..15953
                     /gene="ubiV"
                     /locus_tag="ACHJ7J_000307"
     CDS             15075..15953
                     /gene="ubiV"
                     /locus_tag="ACHJ7J_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO4998591.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16034..17041
                     /gene="yhbW"
                     /locus_tag="ACHJ7J_000308"
     CDS             16034..17041
                     /gene="yhbW"
                     /locus_tag="ACHJ7J_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO4998592.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17158..18402)
                     /gene="mtr"
                     /locus_tag="ACHJ7J_000309"
     CDS             complement(17158..18402)
                     /gene="mtr"
                     /locus_tag="ACHJ7J_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO4998593.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18556..20445)
                     /gene="deaD"
                     /locus_tag="ACHJ7J_000310"
     CDS             complement(18556..20445)
                     /gene="deaD"
                     /locus_tag="ACHJ7J_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO4998594.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20438..20518)
                     /gene="yrbN"
                     /locus_tag="ACHJ7J_000311"
     CDS             complement(20438..20518)
                     /gene="yrbN"
                     /locus_tag="ACHJ7J_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO4998595.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20625..21509)
                     /gene="nlpI"
                     /locus_tag="ACHJ7J_000312"
     CDS             complement(20625..21509)
                     /gene="nlpI"
                     /locus_tag="ACHJ7J_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO4998596.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21618..23753)
                     /gene="pnp"
                     /locus_tag="ACHJ7J_000313"
     CDS             complement(21618..23753)
                     /gene="pnp"
                     /locus_tag="ACHJ7J_000313"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO4998597.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24000..24269)
                     /gene="rpsO"
                     /locus_tag="ACHJ7J_000314"
     CDS             complement(24000..24269)
                     /gene="rpsO"
                     /locus_tag="ACHJ7J_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO4998598.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24418..25362)
                     /gene="truB"
                     /locus_tag="ACHJ7J_000315"
     CDS             complement(24418..25362)
                     /gene="truB"
                     /locus_tag="ACHJ7J_000315"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO4998599.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25362..25763)
                     /gene="rbfA"
                     /locus_tag="ACHJ7J_000316"
     CDS             complement(25362..25763)
                     /gene="rbfA"
                     /locus_tag="ACHJ7J_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO4998600.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25928..28600)
                     /gene="infB"
                     /locus_tag="ACHJ7J_000317"
     CDS             complement(25928..28600)
                     /gene="infB"
                     /locus_tag="ACHJ7J_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO4998601.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28625..30112)
                     /gene="nusA"
                     /locus_tag="ACHJ7J_000318"
     CDS             complement(28625..30112)
                     /gene="nusA"
                     /locus_tag="ACHJ7J_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO4998602.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30140..30592)
                     /gene="rimP"
                     /locus_tag="ACHJ7J_000319"
     CDS             complement(30140..30592)
                     /gene="rimP"
                     /locus_tag="ACHJ7J_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO4998603.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30799..30875)
                     /locus_tag="ACHJ7J_000320"
     tRNA            complement(30799..30875)
                     /locus_tag="ACHJ7J_000320"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30839..30841),aa:Met,seq:cat)
     gene            31223..32566
                     /gene="argG"
                     /locus_tag="ACHJ7J_000321"
     CDS             31223..32566
                     /gene="argG"
                     /locus_tag="ACHJ7J_000321"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO4998604.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVDNLENKGQ"
     gene            complement(32574..34199)
                     /gene="yhbX"
                     /locus_tag="ACHJ7J_000322"
     CDS             complement(32574..34199)
                     /gene="yhbX"
                     /locus_tag="ACHJ7J_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4998605.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34659..34745)
                     /locus_tag="ACHJ7J_000323"
     tRNA            complement(34659..34745)
                     /locus_tag="ACHJ7J_000323"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34709..34711),aa:Leu,seq:gag)
     gene            complement(34760..35092)
                     /gene="secG"
                     /locus_tag="ACHJ7J_000324"
     CDS             complement(34760..35092)
                     /gene="secG"
                     /locus_tag="ACHJ7J_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO4998606.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35320..36657)
                     /gene="glmM"
                     /locus_tag="ACHJ7J_000325"
     CDS             complement(35320..36657)
                     /gene="glmM"
                     /locus_tag="ACHJ7J_000325"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO4998607.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36650..37498)
                     /gene="folP"
                     /locus_tag="ACHJ7J_000326"
     CDS             complement(36650..37498)
                     /gene="folP"
                     /locus_tag="ACHJ7J_000326"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO4998608.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37588..39522)
                     /gene="ftsH"
                     /locus_tag="ACHJ7J_000327"
     CDS             complement(37588..39522)
                     /gene="ftsH"
                     /locus_tag="ACHJ7J_000327"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO4998609.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39622..40251)
                     /gene="rlmE"
                     /locus_tag="ACHJ7J_000328"
     CDS             complement(39622..40251)
                     /gene="rlmE"
                     /locus_tag="ACHJ7J_000328"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO4998610.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40377..40670
                     /gene="yhbY"
                     /locus_tag="ACHJ7J_000329"
     CDS             40377..40670
                     /gene="yhbY"
                     /locus_tag="ACHJ7J_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO4998611.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40826..41302)
                     /gene="greA"
                     /locus_tag="ACHJ7J_000330"
     CDS             complement(40826..41302)
                     /gene="greA"
                     /locus_tag="ACHJ7J_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO4998612.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41550..42983
                     /gene="dacB"
                     /locus_tag="ACHJ7J_000331"
     CDS             41550..42983
                     /gene="dacB"
                     /locus_tag="ACHJ7J_000331"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4998613.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43023..44195)
                     /gene="cgtA"
                     /locus_tag="ACHJ7J_000332"
     CDS             complement(43023..44195)
                     /gene="cgtA"
                     /locus_tag="ACHJ7J_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO4998614.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44211..45176)
                     /gene="yhbE"
                     /locus_tag="ACHJ7J_000333"
     CDS             complement(44211..45176)
                     /gene="yhbE"
                     /locus_tag="ACHJ7J_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4998615.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45303..45560)
                     /gene="rpmA"
                     /locus_tag="ACHJ7J_000334"
     CDS             complement(45303..45560)
                     /gene="rpmA"
                     /locus_tag="ACHJ7J_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO4998616.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45581..45892)
                     /gene="rplU"
                     /locus_tag="ACHJ7J_000335"
     CDS             complement(45581..45892)
                     /gene="rplU"
                     /locus_tag="ACHJ7J_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO4998617.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46151..47122
                     /gene="ispB"
                     /locus_tag="ACHJ7J_000336"
     CDS             46151..47122
                     /gene="ispB"
                     /locus_tag="ACHJ7J_000336"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO4998618.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPKEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47351..47629
                     /gene="sfsB"
                     /locus_tag="ACHJ7J_000337"
     CDS             47351..47629
                     /gene="sfsB"
                     /locus_tag="ACHJ7J_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO4998619.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47677..48936)
                     /gene="murA"
                     /locus_tag="ACHJ7J_000338"
     CDS             complement(47677..48936)
                     /gene="murA"
                     /locus_tag="ACHJ7J_000338"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO4998620.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48991..49245)
                     /gene="ibaG"
                     /locus_tag="ACHJ7J_000339"
     CDS             complement(48991..49245)
                     /gene="ibaG"
                     /locus_tag="ACHJ7J_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO4998621.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49405..49698)
                     /gene="mlaB"
                     /locus_tag="ACHJ7J_000340"
     CDS             complement(49405..49698)
                     /gene="mlaB"
                     /locus_tag="ACHJ7J_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO4998622.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49698..50333)
                     /gene="mlaC"
                     /locus_tag="ACHJ7J_000341"
     CDS             complement(49698..50333)
                     /gene="mlaC"
                     /locus_tag="ACHJ7J_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO4998623.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50352..50903)
                     /gene="mlaD"
                     /locus_tag="ACHJ7J_000342"
     CDS             complement(50352..50903)
                     /gene="mlaD"
                     /locus_tag="ACHJ7J_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO4998624.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50908..51690)
                     /gene="mlaE"
                     /locus_tag="ACHJ7J_000343"
     CDS             complement(50908..51690)
                     /gene="mlaE"
                     /locus_tag="ACHJ7J_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO4998625.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51698..52507)
                     /gene="mlaF"
                     /locus_tag="ACHJ7J_000344"
     CDS             complement(51698..52507)
                     /gene="mlaF"
                     /locus_tag="ACHJ7J_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO4998626.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAKALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52717..53694
                     /gene="yrbG"
                     /locus_tag="ACHJ7J_000345"
     CDS             52717..53694
                     /gene="yrbG"
                     /locus_tag="ACHJ7J_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO4998627.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53708..54694
                     /gene="kdsD"
                     /locus_tag="ACHJ7J_000346"
     CDS             53708..54694
                     /gene="kdsD"
                     /locus_tag="ACHJ7J_000346"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO4998628.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54715..55281
                     /gene="kdsC"
                     /locus_tag="ACHJ7J_000347"
     CDS             54715..55281
                     /gene="kdsC"
                     /locus_tag="ACHJ7J_000347"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO4998629.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55278..55853
                     /gene="lptC"
                     /locus_tag="ACHJ7J_000348"
     CDS             55278..55853
                     /gene="lptC"
                     /locus_tag="ACHJ7J_000348"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO4998630.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55822..56379
                     /gene="lptA"
                     /locus_tag="ACHJ7J_000349"
     CDS             55822..56379
                     /gene="lptA"
                     /locus_tag="ACHJ7J_000349"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO4998631.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56386..57111
                     /gene="lptB"
                     /locus_tag="ACHJ7J_000350"
     CDS             56386..57111
                     /gene="lptB"
                     /locus_tag="ACHJ7J_000350"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO4998632.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57159..58592
                     /gene="rpoN"
                     /locus_tag="ACHJ7J_000351"
     CDS             57159..58592
                     /gene="rpoN"
                     /locus_tag="ACHJ7J_000351"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO4998633.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58615..58902
                     /gene="hpf"
                     /locus_tag="ACHJ7J_000352"
     CDS             58615..58902
                     /gene="hpf"
                     /locus_tag="ACHJ7J_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO4998634.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59020..59511
                     /gene="ptsN"
                     /locus_tag="ACHJ7J_000353"
     CDS             59020..59511
                     /gene="ptsN"
                     /locus_tag="ACHJ7J_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO4998635.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59557..60411
                     /gene="rapZ"
                     /locus_tag="ACHJ7J_000354"
     CDS             59557..60411
                     /gene="rapZ"
                     /locus_tag="ACHJ7J_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO4998636.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60408..60680
                     /gene="npr"
                     /locus_tag="ACHJ7J_000355"
     CDS             60408..60680
                     /gene="npr"
                     /locus_tag="ACHJ7J_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO4998637.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60894..61526
                     /gene="yrbL"
                     /locus_tag="ACHJ7J_000356"
     CDS             60894..61526
                     /gene="yrbL"
                     /locus_tag="ACHJ7J_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO4998638.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61523..62251)
                     /gene="mtgA"
                     /locus_tag="ACHJ7J_000357"
     CDS             complement(61523..62251)
                     /gene="mtgA"
                     /locus_tag="ACHJ7J_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO4998639.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62248..62901)
                     /gene="elbB"
                     /locus_tag="ACHJ7J_000358"
     CDS             complement(62248..62901)
                     /gene="elbB"
                     /locus_tag="ACHJ7J_000358"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO4998640.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63131..65467)
                     /gene="arcB"
                     /locus_tag="ACHJ7J_000359"
     CDS             complement(63131..65467)
                     /gene="arcB"
                     /locus_tag="ACHJ7J_000359"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO4998641.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65563..66492)
                     /gene="yhcC"
                     /locus_tag="ACHJ7J_000360"
     CDS             complement(65563..66492)
                     /gene="yhcC"
                     /locus_tag="ACHJ7J_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO4998642.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            67167..71627
                     /gene="gltB"
                     /locus_tag="ACHJ7J_000361"
     CDS             67167..71627
                     /gene="gltB"
                     /locus_tag="ACHJ7J_000361"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO4998643.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTQAIAKNYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71640..73058
                     /gene="gltD"
                     /locus_tag="ACHJ7J_000362"
     CDS             71640..73058
                     /gene="gltD"
                     /locus_tag="ACHJ7J_000362"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO4998644.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73242..74369
                     /gene="yhcG"
                     /locus_tag="ACHJ7J_000363"
     CDS             73242..74369
                     /gene="yhcG"
                     /locus_tag="ACHJ7J_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO4998645.1"
                     /translation="MESLSEGTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRLR"
     gene            complement(74429..74893)
                     /gene="nanQ"
                     /locus_tag="ACHJ7J_000364"
     CDS             complement(74429..74893)
                     /gene="nanQ"
                     /locus_tag="ACHJ7J_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO4998646.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74890..75765)
                     /gene="nanK"
                     /locus_tag="ACHJ7J_000365"
     CDS             complement(74890..75765)
                     /gene="nanK"
                     /locus_tag="ACHJ7J_000365"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO4998647.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADVKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75762..76451)
                     /gene="nanE"
                     /locus_tag="ACHJ7J_000366"
     CDS             complement(75762..76451)
                     /gene="nanE"
                     /locus_tag="ACHJ7J_000366"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO4998648.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPDIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNAAMKKAVL"
     gene            complement(76499..77989)
                     /gene="nanT"
                     /locus_tag="ACHJ7J_000367"
     CDS             complement(76499..77989)
                     /gene="nanT"
                     /locus_tag="ACHJ7J_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO4998649.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78098..78991)
                     /gene="nanA"
                     /locus_tag="ACHJ7J_000368"
     CDS             complement(78098..78991)
                     /gene="nanA"
                     /locus_tag="ACHJ7J_000368"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO4998650.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79113..79904)
                     /gene="nanR"
                     /locus_tag="ACHJ7J_000369"
     CDS             complement(79113..79904)
                     /gene="nanR"
                     /locus_tag="ACHJ7J_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO4998651.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            80284..81651
                     /gene="dcuC"
                     /locus_tag="ACHJ7J_000370"
     CDS             80284..81651
                     /gene="dcuC"
                     /locus_tag="ACHJ7J_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4998652.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHKLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(81694..82191)
                     /gene="sspB"
                     /locus_tag="ACHJ7J_000371"
     CDS             complement(81694..82191)
                     /gene="sspB"
                     /locus_tag="ACHJ7J_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO4998653.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(82197..82835)
                     /gene="sspA"
                     /locus_tag="ACHJ7J_000372"
     CDS             complement(82197..82835)
                     /gene="sspA"
                     /locus_tag="ACHJ7J_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO4998654.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(83230..83622)
                     /gene="rpsI"
                     /locus_tag="ACHJ7J_000373"
     CDS             complement(83230..83622)
                     /gene="rpsI"
                     /locus_tag="ACHJ7J_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO4998655.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(83638..84066)
                     /gene="rplM"
                     /locus_tag="ACHJ7J_000374"
     CDS             complement(83638..84066)
                     /gene="rplM"
                     /locus_tag="ACHJ7J_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO4998656.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(84285..85412)
                     /gene="zapE"
                     /locus_tag="ACHJ7J_000375"
     CDS             complement(84285..85412)
                     /gene="zapE"
                     /locus_tag="ACHJ7J_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO4998657.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDEIRAQMDKLWLALAGAKREISPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            85606..86004
                     /gene="zapG"
                     /locus_tag="ACHJ7J_000376"
     CDS             85606..86004
                     /gene="zapG"
                     /locus_tag="ACHJ7J_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO4998658.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            86158..87525
                     /gene="degQ"
                     /locus_tag="ACHJ7J_000377"
     CDS             86158..87525
                     /gene="degQ"
                     /locus_tag="ACHJ7J_000377"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO4998659.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            87615..88682
                     /gene="degS"
                     /locus_tag="ACHJ7J_000378"
     CDS             87615..88682
                     /gene="degS"
                     /locus_tag="ACHJ7J_000378"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO4998660.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(88745..89683)
                     /gene="mdh"
                     /locus_tag="ACHJ7J_000379"
     CDS             complement(88745..89683)
                     /gene="mdh"
                     /locus_tag="ACHJ7J_000379"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4998661.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            90118..90588
                     /gene="argR"
                     /locus_tag="ACHJ7J_000380"
     CDS             90118..90588
                     /gene="argR"
                     /locus_tag="ACHJ7J_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO4998662.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            90953..91216
                     /gene="yhcN"
                     /locus_tag="ACHJ7J_000381"
     CDS             90953..91216
                     /gene="yhcN"
                     /locus_tag="ACHJ7J_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO4998663.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(91272..91544)
                     /gene="yhcO"
                     /locus_tag="ACHJ7J_000382"
     CDS             complement(91272..91544)
                     /gene="yhcO"
                     /locus_tag="ACHJ7J_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO4998664.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(91636..93603)
                     /gene="aaeB"
                     /locus_tag="ACHJ7J_000383"
     CDS             complement(91636..93603)
                     /gene="aaeB"
                     /locus_tag="ACHJ7J_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO4998665.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(93609..94541)
                     /gene="aaeA"
                     /locus_tag="ACHJ7J_000384"
     CDS             complement(93609..94541)
                     /gene="aaeA"
                     /locus_tag="ACHJ7J_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO4998666.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(94549..94752)
                     /gene="aaeX"
                     /locus_tag="ACHJ7J_000385"
     CDS             complement(94549..94752)
                     /gene="aaeX"
                     /locus_tag="ACHJ7J_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO4998667.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            94935..95864
                     /gene="aaeR"
                     /locus_tag="ACHJ7J_000386"
     CDS             94935..95864
                     /gene="aaeR"
                     /locus_tag="ACHJ7J_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO4998668.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(95992..97437)
                     /gene="tldD"
                     /locus_tag="ACHJ7J_000387"
     CDS             complement(95992..97437)
                     /gene="tldD"
                     /locus_tag="ACHJ7J_000387"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO4998669.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(97776..101576)
                     /gene="yhdP"
                     /locus_tag="ACHJ7J_000388"
     CDS             complement(97776..101576)
                     /gene="yhdP"
                     /locus_tag="ACHJ7J_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO4998670.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKSAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(101644..103113)
                     /gene="rng"
                     /locus_tag="ACHJ7J_000389"
     CDS             complement(101644..103113)
                     /gene="rng"
                     /locus_tag="ACHJ7J_000389"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO4998671.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(103103..103696)
                     /gene="yhdE"
                     /locus_tag="ACHJ7J_000390"
     CDS             complement(103103..103696)
                     /gene="yhdE"
                     /locus_tag="ACHJ7J_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO4998672.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVTQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(103705..104193)
                     /gene="mreD"
                     /locus_tag="ACHJ7J_000391"
     CDS             complement(103705..104193)
                     /gene="mreD"
                     /locus_tag="ACHJ7J_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO4998673.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(104193..105296)
                     /gene="mreC"
                     /locus_tag="ACHJ7J_000392"
     CDS             complement(104193..105296)
                     /gene="mreC"
                     /locus_tag="ACHJ7J_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO4998674.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNTATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(105362..106405)
                     /gene="mreB"
                     /locus_tag="ACHJ7J_000393"
     CDS             complement(105362..106405)
                     /gene="mreB"
                     /locus_tag="ACHJ7J_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO4998675.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(106710..108650)
                     /gene="csrD"
                     /locus_tag="ACHJ7J_000394"
     CDS             complement(106710..108650)
                     /gene="csrD"
                     /locus_tag="ACHJ7J_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO4998676.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPTTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            108802..109776
                     /gene="yhdH"
                     /locus_tag="ACHJ7J_000395"
     CDS             108802..109776
                     /gene="yhdH"
                     /locus_tag="ACHJ7J_000395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO4998677.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            110754..111224
                     /gene="accB"
                     /locus_tag="ACHJ7J_000396"
     CDS             110754..111224
                     /gene="accB"
                     /locus_tag="ACHJ7J_000396"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO4998678.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            111235..112584
                     /gene="accC"
                     /locus_tag="ACHJ7J_000397"
     CDS             111235..112584
                     /gene="accC"
                     /locus_tag="ACHJ7J_000397"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO4998679.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            112693..112935
                     /gene="yhdT"
                     /locus_tag="ACHJ7J_000398"
     CDS             112693..112935
                     /gene="yhdT"
                     /locus_tag="ACHJ7J_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO4998680.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            112925..114376
                     /gene="panF"
                     /locus_tag="ACHJ7J_000399"
     CDS             112925..114376
                     /gene="panF"
                     /locus_tag="ACHJ7J_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO4998681.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFSRYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            114388..115269
                     /gene="prmA"
                     /locus_tag="ACHJ7J_000400"
     CDS             114388..115269
                     /gene="prmA"
                     /locus_tag="ACHJ7J_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO4998682.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVIANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            115598..116563
                     /gene="dusB"
                     /locus_tag="ACHJ7J_000401"
     CDS             115598..116563
                     /gene="dusB"
                     /locus_tag="ACHJ7J_000401"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO4998683.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            116589..116885
                     /gene="fis"
                     /locus_tag="ACHJ7J_000402"
     CDS             116589..116885
                     /gene="fis"
                     /locus_tag="ACHJ7J_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO4998684.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(117667..117948)
                     /locus_tag="ACHJ7J_000403"
     CDS             complement(117667..117948)
                     /locus_tag="ACHJ7J_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998685.1"
                     /translation="MTETDLLKIIRRITGISQQPDEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKKPERLRR"
BASE COUNT        28934 a        31685 c        29359 g        28026 t
ORIGIN      
        1 taaggttata aatgaataaa gttacaaaaa cagctattgc gggcttactt gcgcttttcg
       61 caggtaatgc cgctgcaacc gatggtgaaa tagtttttga tggcgagatt ttaaaatccg
      121 cttgtgaaat caatgactct gataagaaaa ttgaagttgc tcttggtcac tataatgccg
      181 aacagtttcg ttctgtaggc gatcgtagcc caaaaattcc attcactatt cctttagtta
      241 actgcccagt gactgggtgg gagcacgata acggcaacgt tgaagcgtct ttccgtttat
      301 ggctggaaac gcgggataat ggtaccgtcc ctaacttccc taatctggca aaagtgggct
      361 cttttgctgg cacagcggca acaggtgtgg gtatccgtat tgacgatgcg gaaagcggaa
      421 accttatgcc actaaatgct atgggcaatg ataacacggt ttatcagatc cctgcggact
      481 ccgctggagt tgttaatgtt gacctcatcg cttactacgt atcaaccgtt gaagcatcag
      541 aaatcacccc aggggaagca gacgctgtcg ttaacgtaac gctggattac cgttaatacg
      601 ttacggcgtt atctgacctt tcagataacg cccttttcct tcctctttct cgttgtatca
      661 ggttgaaaaa taactatgtc aaaacgaaca ttcgcggtga taataacctt gttgtgtagc
      721 ttctgtatcg gccaggcgct tgcagggggt atcgttttac agcgaacgcg agtgatctat
      781 gatgccagcc gcaaagaggc tgcgttacct gtcgcaaaca aaggcgcaga aacgccttat
      841 ttactgcaat catgggtaga taatatagat ggtacaagcc gtgccccatt tattataacc
      901 ccaccgctat ttcgtcttga agctggcgat gattcatcac tgcgaattat taaaacagct
      961 gataacctgc ctgaaaataa agagtcgctg ttctacatta atgttcgtgc cattccagca
     1021 aagaaaaaat cagatgatgt taatgctaac gagttgacgc tggtatttaa aacacggatc
     1081 aaaatgtttt atcgccccgc acacttgaag gggttggtca acgatgcgtg gaaatcactg
     1141 gaatttaaac gtagtgacca ttcactcaat atatataacc caactgaata ttacgtcgta
     1201 tttgccggac tggcagtcga taaagccgat ctcacaagca aaattgaata tatcgcgccc
     1261 ggagaacata aacagttacc acttccagca tctggcggaa agaacgtgaa atgggctgcg
     1321 atcaatgatt atggcggcag ttccgggaca gaaactcgtc cactgcaata acaaatataa
     1381 aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac gcacaccgaa
     1441 acagttggcg ctcattatca aacgctgttt gccgatggtg ctcactggca gcggcatgct
     1501 ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg aaaccacaaa
     1561 aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac aactaccagg
     1621 aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa aaattacatt
     1681 taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac tacgtgagct
     1741 gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata gcgtgatcaa
     1801 ctcgcttgaa caaatcattc ccggtacagc tgctgaattt gatttcaatc atcagcgact
     1861 taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg tctccccttc
     1921 tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag gttctgataa
     1981 ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa atggtgctaa
     2041 ttttggcccc tggcgattac gtaactattc tacgtggaca cgcaacgatc aggcgtcaag
     2101 ctggaacact atcagtagtt atttacaacg tgatatcaag gcgttgaagt ctcagttgct
     2161 tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta ctggcgtgca
     2221 actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc caacggtacg
     2281 cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg tgatctatca
     2341 aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt ccaacagcgg
     2401 cgatttagaa gtcacgattg aagaaagtga cggtacacaa cgtcgcttta tccagcctta
     2461 ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga ccgctggacg
     2521 ctatcgcgct gatgcaaaca gtgatagcaa ggaacccgaa tttgctgaag ccacggcaat
     2581 atatggtttg aataatactt ttacgctgta tggcggcctg ctcggttctg aagattatta
     2641 tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt cgatggatat
     2701 caacagagct gacacccaat tcgataacca gcactctttt catggctatc aatggcgtac
     2761 gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct actatcgcta
     2821 taccaacgat ggctatttta gttttgatga agccaatacc cgcaattggg actataacag
     2881 tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg gggtaagtct
     2941 gtatgcctcc ggttcacagc aagactattg gggcaataac gagaaaaaca ggaatatctc
     3001 tgttggggtt tccggccagc aatggggaat tggttacagc ctgaattatc aatacagccg
     3061 ctacactgat caaaataatg accgcgcact ctctttgaat ctcagtattc cgttagaacg
     3121 ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc gcccaaccca
     3181 acatgaaatg cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga
     3241 acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc
     3301 ttatggaacc ttcagtgccg gatacagtta cggtaatgac agcagccaat acaattacgg
     3361 cgttaccggc ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa
     3421 cgcttttgcg cttatcgatg ctaacggggc atctggcgtg aggatacaaa actatccggg
     3481 gattgctacc gatccctttg gctatgcagt ggttccttat ctcacaactt atcaggaaaa
     3541 ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgaac aaacaacaca
     3601 gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta
     3661 tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc
     3721 cagcaacgat gatacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc
     3781 tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcaaca
     3841 atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac
     3901 agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt
     3961 tgatatccac atcctgcgct tatgcctcct caggagggtg tggagccgac agcactagcg
     4021 gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca gataacgtga
     4081 caggacggga gtttacctct gcaacgctaa gtagcactaa ctggcaatac gcctgttcct
     4141 gctctgcggg taaggcagtt aaacttgtct atatggtcag ccccgtactt accaccactg
     4201 gacatcagac aggatattac aaactcaatg acagcctgga tattaaaacc acattacagg
     4261 caaacgacat tccaggactc acaaccgacc aggttgtctc tgttaacacc cgattcacac
     4321 agataaaaag cagcaccgta tattctgctg caacccaaac gggtgtttgc cagggtgaca
     4381 cgtctcgtta tggacccgtt aatattggtg caaataccac ctttaccctg tatgtcacca
     4441 agccatttct cggctcgatg accattccga aaacggatat tgccgtcatt aaaggcgcgt
     4501 gggtcgatgg aatgggaagc ccgtctacag gtgacttcca tgatttagtc aagttatcga
     4561 ttcagggaaa tctcaccgcc ccacagtcgt gcaaaattaa tcagggcgat gttattaagg
     4621 ttaattttgg attcatcaat ggtcagaagt ttaccacccg caatgccatg ccagacggtt
     4681 ttactccagt agactttgat atcacttatg actgtggtga tacttcaaag attaaaaact
     4741 cgttgcaaat gcgcatcgac ggtacaactg gggtagtaga ccagtacaac ctggtcgcca
     4801 gacgaagaag ttcagacaat gcgcccgatg tcggtattcg tattgaaaat ctcggcggcg
     4861 gagttgcaaa tattcctttt cagaacggta tccttcccgt tgatccttcc gggcatggca
     4921 ccgtcaatat gcgcgcctgg ccagttaatc tggtcggtgg tgagctggaa acaggaaaat
     4981 ttcagggcac agccaccatt accgtcatcg tgcggtaaaa gaagacgttg cgttttgatt
     5041 aacgcaacgc ttcggcactg ttacccctgc tgctccagcg catacttata cagcgcattt
     5101 ttcttcacgc cgtgaatttc tgcggccagc gccgccgctt ttttcagcgg cagttctgcc
     5161 tgtagcagcg ccagcgtgcg cagggcatcg gcgggtaagt cttcttcctg tgctttatga
     5221 ccttcgacaa tcagcaccat ttcgcctttg cgacggtttt catcttcctt tacccacgcc
     5281 agcagctcgc caacgggcgc gccgtgaatg gtttcccagg ttttggtcag ctcacgcgcc
     5341 agaaccacgt agcgggattc gcctaatacc gcaacgatat cttccaggct atctaacaga
     5401 cggtgggtag attcataaaa aatcagcgtg cgcggctccg cttcaatggc ttttagcgca
     5461 tcacggcggc cttttgattt ggcaggtaaa aagccttcgt aacagaaacg gtcagagggt
     5521 aaacccgcgg cgcttaacgc agtgatagca gcacacggtc cgggtagcgg caccacacga
     5581 atccccgctt cacggcaggt acgcaccaga tggtagccag gatcgttaat tagcggcgtt
     5641 ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca gcttcgccag cagcgtttcg
     5701 gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc gggcattaat cccaaaatgt
     5761 tgcagcaata aaccggtgtg atgagtatcc tcggcggcaa tcagatcaac ggcctgtaat
     5821 acctctaacg cacgctgggt gatatccgcc agattgccga ttggcgtcgg tacaatgtaa
     5881 agctggccct gagaattatc cgccgattgg tgttgtttca ttgtgtcgtc cgtattgccg
     5941 atttaatatt gagcattgcg taaaaaaaat atcactggat acattatggt accctcaaca
     6001 ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct gattttcgcc
     6061 ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac ggcgcaggct
     6121 gattctgcct tttatcttca gcagatgcag caaagctctg atgataccag gatcaactgg
     6181 caattactcg ccattcgtgc actggtgaaa gaaggtaaaa ccgggcaggc ggttgagttg
     6241 tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac actgctggcg
     6301 gtagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatt
     6361 acacccgccg atttagaaca aaaccagcaa gcccgttact ggcaggcaaa aatcgatgcc
     6421 agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga accgctgctt
     6481 ggcgcgaaag aaaaacagca gaatattgat gccacctggc aggcgctctc ctccatgact
     6541 caggaacagg cgaatacgct ggtgatcaac gccgacgaaa atattctgca aggctggctg
     6601 gatctgcagc gcgtctggtt tgataaccgt aacgatcccg acatgatgaa agccgggatc
     6661 gccgactggc agaaacgtta tccgaacaac ccgggcgcga aaatgctgcc aacgcagttg
     6721 gttaacgtaa aagcgtttaa accagcctcg accaacaaaa tcgccctgct gttgccactg
     6781 aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc ggcgaaaaat
     6841 atcggcactc agccagtggc agctcaggta gctgccgcac ctgccgcaga cgtagcagaa
     6901 caaccccaac cgcaaaccgt ggatggcgtt gccagcccgg cacaagcctc ggttagcgat
     6961 ctgaccggtg aacagcctgc agcccagccg gtgcctgtaa gcgccccggc gacaagcacc
     7021 gcagcggtaa gcgcacccgc aaatccatcc gcagagctga aaatctacga tacctcatca
     7081 caaccactta gccagatctt aagccaggtt cagcaggatg gcgcgagtat tgtggtcggt
     7141 ccgttgctga aaaataacgt tgaagagttg ctgaagagca acactccgct gaacgtactg
     7201 gcactgaacc agccggagaa tatcgaaaat cgcgtcaata tttgttactt cgcgctttca
     7261 ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa acaagcgccg
     7321 ctggtgctga tcccacgcag ttcattgggc gatcgcgtag ccaatgcgtt tgcgcaagag
     7381 tggcagaaac tgggcggcgg caccgttctg caacaaaaat ttggttccac cagcgaatta
     7441 cgcgcgggtg ttaacggcgg ttctggtatt gctttaacgg gtagcccgat tactctcaga
     7501 gcgacaaccg actccggcat gacgaccaac aatccaacgc tgcaaaccac gccaaccgat
     7561 gaccagttca ccaataatgg cggtcgtgtc gatgcggtgt acattgtggc aacgccgggt
     7621 gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag cggtgcaacg
     7681 ctgtacgcta gctcccgcag tgcgcaaggg accgctggcc cggatttccg actggagatg
     7741 gaaggcttgc agtacagcga aatcccgatg ctggcaggcg gtaatctacc gttaatgcag
     7801 caggcactca gcgcggtgaa taacgattat tcactggctc gcatgtatgc gatgggcgtc
     7861 gatgcctggt cgctggcaaa tcatttctca caaatgcgcc aggttcaggg ttttgaaatc
     7921 aacggtaata ccggaagcct gacggctaac ccggattgcg tgattaacag gaagttatca
     7981 tggctacagt accaacaagg tcaggtagtc cccgccagtt aaccaccaaa cagaccggcg
     8041 atgcgtggga agtacaagcg cgtcgctggc tggaaggcaa aggactgcga tttgtcgccg
     8101 ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc cggaccaccg
     8161 tttttgtcga ggtgcgttac cgccgttctg cgctttatgg cggcgcggca gccagtgtga
     8221 cccgcagcaa acaacacaaa ttattacaga ctgcccgctt gtggctcgcg cgtcataatg
     8281 ggagttttga tactgtggat tgccggttcg atgtggtagc cttcaccggg aatgaggttg
     8341 agtggattaa ggatgccttt aatgaccact cataattaag gtttaaggat tagcgtgcaa
     8401 gaaagaatta aagcttgctt cactgaaagc attcaaactc aaattgcggc ggcagaggcg
     8461 cttccggatg ccatctcccg tgcggccatg acgctggttc agtctctgct caatggcaac
     8521 aaaatcctct gttgtggtaa tggaacttcc gctgccaatg cacagcattt tgctgccagc
     8581 atgatcaacc gtttcgaaac ggagcggccc agcttacctg ccattgcact aaatactgat
     8641 aatgttgtct taacggcgat tgccaacgat cgcttacatg atgaagtgta tgcaaaacag
     8701 gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca tttccacccg tggcaacagc
     8761 cgcgatattg taaaagcggt agaagctgcc gttacgcgtg atatgaccat tgtggcattg
     8821 accggctatg acggcggtga acttgcaggt ttgttagggc cacaggatgt ggagatccgc
     8881 atcccttcgc atcacagtgc tcgcattcag gaaatgcata tgctgacggt aaattgcctg
     8941 tgcgatctga tcgataacac gcttttccct caccaggatg attaaggaga atacatgaag
     9001 gcattatcgc caatcgcagt ccttatttcc gcgctgctgt tgcaaggttg tgttgccgct
     9061 gccgtagtgg gtactgctgc tgtgggtacc aaagccgcaa ctgacccacg cagtgtcggc
     9121 acccaggtgg acgatggtac cctggaagtg cgcgtgaaca gcgcattgtc gaaagacgaa
     9181 cagattaaga aagaagcgcg cattaatgta acggcctatc agggcaaagt gctgctggtt
     9241 gggcagtcac caaacgctga actttcggct cgcgccaaac agattgctat gggcgtagac
     9301 ggtgccaacg aagtgtataa cgagattcgt cagggccagc cgattggtct gggcgaagcg
     9361 tctaacgata cgtggatcac caccaaagtg cgttcgcagc tcttaaccag cgacctggtg
     9421 aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat ggggctggtg
     9481 actgaacgtg aagcgaaagc ggcggcagat attgccagcc gggtgagcgg cgtgaagcgg
     9541 gtaactacgg cgtttacgtt tattaaatag cagccccttg taacgcctga tgcgacgctt
     9601 gccgcgtctt atcaggccta caacgatgca aaccgtaggt cggataaggc gtttacgccg
     9661 catccgacat gttttccctc aaatcagaat aacagcgcca gcccgccgac aatcacgcct
     9721 gacactgcca ccatcgcccc cgtcagccat aaggctttcg ccgggaacgc tttgcgcagc
     9781 ataatcagtg acggcaaact caccgccggg agcgtcatca acaatgccag cgccggagca
     9841 gttcccatac ctgccagcat catcgtttgt acaatcggaa tttctgctgc cgtgggaatg
     9901 acaaacaagc accctgctac cgccatcgcc accacccaca tcaggctgtt atcgacagca
     9961 ccatcggcat gggggaataa ccagacgcga gcggcaccta acaccagtac tgcaaggatg
    10021 taaaccggga tcgtactcca gaaaagcgtc catagcgccc tgccccagcg gctaaaaaat
    10081 ccgccctgtg cttccggtat gtcaatttcg accggtgctt gcgtttgcgg tgtttcacgc
    10141 acccattttt gcaccagcgt cgcaatcagc aacaccatca ccagcccggc caccagacga
    10201 atcgccgcaa aaccccagcc gaggacaaac cccataaaca ccagcgtcgc cgggtttaac
    10261 accggattgc ccatccagaa tgccagcgca ccgcccatcg acacctgttg gcgacgcatt
    10321 cccgccgcga ccggagccgc acagcaggta cacatcatgc ccggcagcga aaacagcgtt
    10381 cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac gcaacaacca gtcacgcggg
    10441 atcagcacct gaatcaacga accgaggatc acccccagca ccgccgcttt ccataccgcg
    10501 aggaaataga tcatcgcgta atccaacgcc gcctgccacg gattagcatc cgcctgcgca
    10561 aggatagatt taccgatact gtgggtttcg gcagcagtaa acgctttgcc gtagtaaggt
    10621 tgccatttca cataccagag accggcaatg acaacgagaa agaaaagcgc gggtttccac
    10681 cactgaatgg gcgttgccgc ctgagatgaa gactgaccag tcatagcatt ccccaggaat
    10741 aagttatgat aattgagcgc gtgaatatta cgctcactat caattcttgg gaataattat
    10801 tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca caccttcatg cccgggccgc
    10861 atcgcctctg ccagcatgac ccgcgccacg tctctcgcat caatggattt ccagttaccg
    10921 ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt gctacgatcg
    10981 cccagtaaca tcgacgggcg agcaatggtc aatttcggcc agttctgggc gattaatgct
    11041 tcttccatct cccctttgac gcggttatag aaaaacggcg agtgggcatt ggcacccata
    11101 gcactgacca ccagcatatg ctgcgcgccc agtcgccgcc cggttaatgc ggtatccact
    11161 accagcgtgt aatcggcatg aataaacgcc tctttgctcc ccgcttctcg ccgcgtggtg
    11221 ccgagacaac aaaacacaat gtcgatggga tcggtgacct gcgccagcgc gtcagtcagt
    11281 tgcggatcat ggggattaaa caccccgggc atatcgccca acggacgtcg cgtcggcgcg
    11341 gctatggcgt taactttcgg ttcgttaatc aacatccgca gcaggtgacc gcccaccagc
    11401 cccgttgcgc ctgtaatcag tacctgactc atcttcgctc ctttacagaa ttgtctgcct
    11461 tgcgctccac ggctcatgca ccaggcttaa tagaccatga ggtaattatc cccgattgtg
    11521 agaaattcgc ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa aattgccgtt
    11581 ttaatcactg atgaatttga agattcagaa tttacttcac ccgcagacga gttccgtaaa
    11641 gccggacacg aagtgattac cattgaaaaa caagcgggta aaacggtgaa aggcaaaaaa
    11701 ggagaagcca gcgtgaccat cgataaatcc atcgatgaag tgacgccagc ggagtttgat
    11761 gccctgttgc taccgggcgg tcattcaccg gattatctgc gtggcgacaa ccgttttgtc
    11821 acctttaccc gtgattttgt gaatagcggc aaacctgttt tcgccatctg tcacggtccg
    11881 cagctgctga tcagcgccga tgtgattcgg gggcgcaaac tgaccgcagt taaaccgatc
    11941 attattgatg ttaaaaatgc gggcgcggaa ttttacgatc aggaagtcgt ggttgataaa
    12001 gatcagctgg ttaccagccg gacgccggac gatctgccag cgtttaaccg cgaggcgtta
    12061 cgcctgctcg gtgcctgagc cgcgtagcca aatcattttt ttaccaaagc ccagcgtgtt
    12121 gtcggtgaat ttgatttcat cgagccggat ttcccacacc ggggccgaca gcattctggc
    12181 aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg ctttcctcac cttccagcct
    12241 gcggatctca cctttaaact gcacgccgcg aattaacgct accgttttcg gctgaccgtt
    12301 taccgttccg gcaaccgcag cctgcggccc actcatctgc gcatgacgcg ttttttcttc
    12361 cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga taaaaagcat tagcgcacca
    12421 gagttcccct tcctgctgca cacaccaggt gacaacatgt tgttttgcca accaacggct
    12481 gatggtgatg agtgtttcca ttgctgttct cttttatact gtgggcctga ccttaacatg
    12541 cgacgcagac gatgacaccc tggtttcttt acttgatccg taccgccgac aataagcttt
    12601 ataccgggat caccacggat gtcgaacgcc gctatcagca gcaccaaagc ggcaaagggg
    12661 cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt ttctgcgcca gtgggcgatc
    12721 gttcgctggc gttacgggcg gaatatcgcg ttaaacaact gaccaaacgg caaaaagagc
    12781 gtctggtagc ggaaggcgca gggtttgcag agctgttaag cagtctgcaa accccggaga
    12841 ttaaaagcga ttgaaatgct cgtgatactc aaccagacca gttacgccat tcaacgcgtc
    12901 atccgctaaa cgatgtacct ggaaggcgct ttcagtgcct ggccagcggc aacgcagatc
    12961 gtgatgtgcc gccagttcaa agccaaaacg gctgtacagc gccggatcgc ccagcgtcac
    13021 cactgcggca tagccgaact cattaagcga atcgagtcct tcatagacca gttggcgtgc
    13081 cagcccctgt ccacggtatt tttcatcaac cgccaacggt gccatgccga cccattgcag
    13141 gtcttcgccc tgcacatcaa ccggactaaa tgccacatag ccaatgacct gaccttcgtc
    13201 atctgtcgcc accagcccca gcgtcagaaa gccatcttca cgcagatcgt gaaccagctt
    13261 cgcttccgca tcactttcga atgagcgacg cagcagggca tcaatacccg gcgcatcaat
    13321 gggaatttct actcgaatta gcatggttca cctaccgatg tctgtttggt ttcaggcgcg
    13381 gttttcattc ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg taaggctttc
    13441 ggcatttgct ccagttcaat ggcgtccatc aggtttttca catacagccc cagctccgta
    13501 tcgccttcaa tcaccagccg acgctggaag aagagcgtat ccggatcctg tttacgcgcc
    13561 gcaatcatca gcagatcgct ggcgtcggca ctaaaactca catcagcttg cgcgttctgg
    13621 ctgaccacca gtttgccatt caccaccgag gtaaaccatt gcaggtcaat atcacgcaca
    13681 tgaatactta accagcggcc ttcaagaaac tccagctcgc catcatccag cgcctggcgg
    13741 aattgccagc ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg cgtcagtttt
    13801 accggtacac tcaacagaga tggccccaaa tgcacaatac gggaacgcag tttatccaac
    13861 acgagtttta ctccctgttt caacaatcat cctattttgc catatcagaa aaataacata
    13921 gcggtataaa tcaacaattc catatgaaat tgctgctacc accaatacaa ctttaactgc
    13981 cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc cagttcgtta acatttttgc
    14041 gttttgatag cgcaaccttc aggaaaaatt atggagctgc tctgccctgc cggaaatctc
    14101 ccggcgctta aggcggccat cgaaaacggc gcagatgctg tttatatcgg gctaaaagat
    14161 gataccaatg cccgtcactt cgccggcctt aactttaccg agaaaaaatt gcaggaagcg
    14221 gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg cgattaacac ttttgcgcat
    14281 ccggacggtt acgcccgttg gcagcgcgcc gtggatatgg cggcgcagct gggtgccgac
    14341 gcgctgatcc tcgccgacct cgccatgctg gagtacgccg ccgagcgtta cccgcatatt
    14401 gagcgtcatg tgtcagtgca ggcttcggcg accaatgaag aggcgattaa cttttatcat
    14461 cgccattttg acgttgctcg cgtggtgctg ccgcgcgtgt tgtcgattca tcaggtgaaa
    14521 caactggcac gggtcacacc tgtcccgctg gaagtgtttg ctttcggcag cctgtgcatt
    14581 atgtcggaag gtcgttgcta tctgtcgtcg tatctgacgg gtgagtcgcc caacaccgtg
    14641 ggcgcgtgtt ctccggcccg tttcgtgcgc tggcagcaaa cgccgcaggg gctggaatcc
    14701 cgcctgaacg aagtgctgat cgaccgttat caggacggcg aaaacgcagg ttatccgacg
    14761 ctatgtaaag ggcgttatct ggtggacggc gagcgctatc acgcgctgga agaaccaacc
    14821 agtctcaata ccctggaact gctgccggag ttaatggcgg cgaatattgc ttcggtgaaa
    14881 attgaaggcc gccaacgtag cccggcgtat gtcagccagg tggcgaaagt ctggcgtcag
    14941 gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac cgcaaagcgc gtggatggag
    15001 acgctcgggt cgatgtccga aggcacgcag accacccttg gcgcgtatca ccgtaaatgg
    15061 cagtgagaag agcaatgaaa tattccttag ggccagtgct gtggtactgg ccaaaagaga
    15121 cgctggaaga tttttatcag caggccgcca ccagcagcgc cgacgtgatt tatcttggcg
    15181 aagcggtttg cagcaagcgc cgggcaacca aagttggcga ctggctggag atggcaaaat
    15241 cgctcgccgg aagtggtaag cagatagtgc tctccacgct ggcgctggtg caggcatcat
    15301 ctgaactggg cgaactgaaa cgctatgttg agaacggtga gtttctgatt gaagccagcg
    15361 atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc ggacacgcgc
    15421 tgaactgcta taacgcggtg acgctgaaaa tattgctcaa acagggcatg atgcgctggt
    15481 gtatgccggt ggagctttcc cgcgactggc tggtgaatct gcttaatcag tgcgatgagc
    15541 tgggcattcg caaccagttt gaagtggaag tcctgagcta cggtcatcta ccgctggcct
    15601 actccgctcg ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag tgtgaaacct
    15661 gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca ggaaaaccaa caagtgtttg
    15721 tactcaatgg cattcagacc atgagcggct acgtttacaa cctcggtaac gagctggcat
    15781 ccatgcaggg cctggttgat gtggtccgcc tgtcaccgca gggtactgac actttcgcga
    15841 tgctcgacgc cttccgcgct aatgaaaatg gcgctgcacc actgccgttg acggcgaaca
    15901 gcgactgtaa cggctactgg cggcggctgg caggactgga gctgcaagcc taagtaaata
    15961 gctcactttg ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac
    16021 aaagtgagct gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat
    16081 tcccgaaggt tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc
    16141 tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc
    16201 cagtgctgcc acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct
    16261 ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg gtcattgcag aacagttcgg
    16321 cacgctcaat acactctatc cggggcgaat cgatttgggg ctgggtcgtg ctccgggtag
    16381 tgaccaacgg acaatgatgg cgctacgtcg tcatatgagc ggcgatattg ataatttccc
    16441 ccgcgatgtg gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg
    16501 cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag
    16561 cgcgcaactg gcggcgcagc ttggtctgcc gtttgcgttt gcctcacact tcgcgccgga
    16621 tatgctgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcag cacggctgga
    16681 aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga
    16741 atttctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccgggca
    16801 actgccgccg ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt
    16861 gcaacaggcg ctgagtatgt cgctggtggg cgataaagcg aaagtgcgtc atggtttgca
    16921 gtcgatcctg cgcgaaaccg acgccgatga gattatggtt aacgggcaga ttttcgacca
    16981 ccaggcgcgc ctgcattcgt ttgagctggc gatggatgtt aaggaagagt tgttgggata
    17041 gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg taggcctgat
    17101 aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc ccgctgatta
    17161 ctgatacacc ggcaacaaat taaagctcga taagatatgg accagtgcgt tgccaacgcc
    17221 aaataccaga atcagcgtaa tcatcggctt gccgccccag acgcggaatt tcgggctgcc
    17281 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    17341 tgctaaacca gcataaccaa tggcgtacag gaatccgttc gggaacagca gaccacccac
    17401 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    17461 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccgagga acgaactcgc
    17521 taccgcaaag tttgagaaca cgaccagcag cagatccaga ctacggctgt tcagtacgcc
    17581 gcttaacgcc tgtaccagca catcaatatt accgcccttc tctgcaatat cgataaactc
    17641 cggacgcggg atgttaccca tcgtcgccag caaccagatg gtatacagcg ccagcgccat
    17701 cagcgtaccg tacaccagac atttcacgat ggttttcgga tctttgccgt aatacttcat
    17761 caggcttggc acgttaccgt gataaccaaa cgatgccaga cagaacggca gggtcatcaa
    17821 cagatacggt gcataagacg cattgctttc ggcgacgttg aacaatgtcg caggctgcac
    17881 atgccccagc aggctaccaa aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    17941 cgctgtcatg cgactgacgg ctttagtgct caaccacacc acaaacgcta ccagcaatgc
    18001 aaaaccaaaa cccgccgccc gtgccgggac gtttagtgac atctctgcga aggtgtgatg
    18061 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaatgcaat
    18121 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    18181 actcgaaccg attctgtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    18241 acagaaccag gtgaagatca gcgccgccat tgaccagaag aaccacgccc cggacatgac
    18301 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    18361 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    18421 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    18481 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gactgtgttc
    18541 gctgttcagc gatgattacg catcgccacc gaaacgacga cgaccggtag agtcatcacg
    18601 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    18661 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    18721 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta attgcatatt
    18781 catcggcttg ttcagaatgc gagtacgtgt aaagtgttgc agcacttcac ccggcatacc
    18841 tttcggcagt tcgatggtgg agtgagaagc aaacagctta atgttaccga tgtaacggct
    18901 gctgatgtcg ccttcgttag caatcgcacc aacgatatga cgaacttcaa caccatcatc
    18961 gcggcccact tcaatacgat acaactgcat gtcaccaaca tcacgacgtt cacgacgcgg
    19021 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    19081 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    19141 ttcaccctgt gccattttca gcagtgcggc ggccagagtt tcgagatcca gctcttcacc
    19201 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    19261 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    19321 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    19381 acgacgctcg cggttctcaa cgaacaacag cgcgcggcca gcacgacccg cacgaccggt
    19441 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    19501 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    19561 acgaccatct ttcaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    19621 cagcgcggcg ctgttgtagc cgttacgctc aagagcttca gccacttcca gagtcgcatt
    19681 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    19741 tgcttcgttt ttacgcatac cccagacagt ccagtagctc tggctgatgt caggacgggt
    19801 agtcacgctg gactgaatgc gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    19861 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    19921 cgccataata gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    19981 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    20041 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    20101 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    20161 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    20221 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    20281 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    20341 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    20401 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    20461 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    20521 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    20581 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    20641 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    20701 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    20761 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    20821 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    20881 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    20941 aagtgctgtt tcaacacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    21001 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    21061 tgcgctaact tgtcacgacc gccgtaatat aatgcgatcc cgcgattcaa gtgcgcgtag
    21121 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    21181 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    21241 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    21301 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    21361 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    21421 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    21481 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    21541 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    21601 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    21661 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    21721 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    21781 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    21841 aatgcgccga agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    21901 tctgcagtga tctcttcgat acgacgaata gcgtgtttcg ctttctcgcc gtcggtcgct
    21961 gcgatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccggtttc ttcggtcagg
    22021 gcacggataa cagagccgcc tttaccgata acatctttga tcttgtccgg gttgatcttg
    22081 atggtatgga tacgcggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    22141 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    22201 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accgtcgcgg
    22261 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    22321 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    22381 ttgattggca cacctgcgtc catcagcgcc agagacgcac cgcacacgga agccatagaa
    22441 gaagaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    22501 atatccggca ttactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    22561 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    22621 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    22681 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    22741 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    22801 ccatcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    22861 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    22921 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    22981 taagcatcgc tcagacgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    23041 accggctccg gctgccagtc ccaacgcggt ttaccggctt ctttcaccag ttcattgatg
    23101 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    23161 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt acccgcaaca
    23221 accagatcca gtttgctctc tttcagctcg tcctgtgtcg ggttcagtac gtactggtca
    23281 ttgatgtaac ctacgcgggc agcaccaatc ggaccattga acggaatacc agacagagac
    23341 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacctgcgg gttaacagaa
    23401 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cgggcgaatc
    23461 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttggacggcc ttcacgacgg
    23521 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    23581 gggaagaagt cctgacctgg tttggctttt ttctgaccaa caacggtaac aaataccgcg
    23641 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    23701 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    23761 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    23821 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    23881 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    23941 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    24001 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    24061 aggtagtcca gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    24121 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    24181 gctacctgaa cttcggtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    24241 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    24301 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    24361 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    24421 cgccgggtat tcaaccacca gacgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    24481 cataccgata aatttgccgt tctcaccttc cgtgacgcga accagtcctt ccagcggcgc
    24541 accagatgta cgaaccgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    24601 caccaccgga tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    24661 cgcagccgga atatcctgct gttcagcttg ctcaacaagt tcacgcaggt gctccagggt
    24721 caccatccgt tcaaccggat atttacttac cgccagacgg cgcaggtaaa taacatgcgc
    24781 gccacagccg agtttctcac ccaggtcgtc aatgatagtg cggatataag tgccttttga
    24841 gcagtgaatt tccagctcca gctcattgcc ttcatggcga ataaacagca attcataaac
    24901 ggtaatcgga cgcgcttcac gcggaacttc aatgccctga cgcgcatatt cgtacagttt
    24961 tttgccctgg tatttcagtg ctgaatacat cgatgggatc tgttcgatat cgccacggaa
    25021 agtatccagt gccgccgcca gttgttctgc actaaaggtt accggacgct cttcaacgat
    25081 ttgtccgtcg gcgtcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    25141 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaatcggcaa
    25201 catgccggtc gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    25261 ccgtttcact ttttgcagcg catcgttgct ggacataccc tgaggtttat ccagcaacaa
    25321 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    25381 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    25441 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    25501 cgcatcgctt tacccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    25561 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    25621 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag acgaggatct
    25681 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    25741 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    25801 tttttaaaaa ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgctag
    25861 cgtcgcaaca ggcaatccat gccggatgcg gcttaaacgc cttatcccgc atagaaccct
    25921 aaaaacctta agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc
    25981 cagtgcggac gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt
    26041 cgttaacgtc atctttgaag cggcgcaggg actccagctc gccttcatag ataaccacgt
    26101 tgtcacgcag aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc
    26161 ctgcgatggc accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa
    26221 tctgctgttt cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca
    26281 ggttatagat gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg
    26341 cagaggcatc agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg
    26401 tggcgtcggt ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt
    26461 cagtagacag tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct
    26521 tcaggacgat attcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga
    26581 gtttagattt ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatagagtg
    26641 caacttcacg cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggaa
    26701 caccggacaa gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac
    26761 ccagttcgtt acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc
    26821 ccttgtgcag agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga
    26881 ggaaggattc gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa
    26941 cttccgcctg cagcaggata gcgtccagca gttcatcgat accggtaccc gctttcgcag
    27001 atacgtgtac gaactggctt tcaccgcccc actcttccgg caggatgccg tactgggaga
    27061 gttcgttctt aacgcgatcc ggatcggctt ctggtttatc gatcttgttc actgcaacca
    27121 ccaccggtac ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcattacgc
    27181 cgtcgtcggc agcaacaacc aggactacga tgtcagttgc ctgcgcacca cgtgcacgca
    27241 ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt
    27301 caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gccactttcg
    27361 ttgagcgaat gtagtccagc agagaggttt taccatggtc aacgtgaccc atgatggtca
    27421 caaccggcgc gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt
    27481 ccagctcgtt ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg
    27541 cggtttcctg atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt
    27601 tgatgacctg agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt
    27661 cgccgatcac aacgtcacgg ttaacggcct gagcaggctt ctggaagcct tgctgcagcg
    27721 aagaaccttt acgttttccg cccttaccgc cacgtactgc tgcgcgtgct tcttcacgat
    27781 cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac
    27841 cacggccacg gccgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt
    27901 gagaagtagt gacgtgataa tcgctggaat cctcagtcgg ttccgcgtta tcagtccatt
    27961 tgttttcttc cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt
    28021 tacgacgcgc ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg
    28081 ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt
    28141 ccgcagcttc acgtttcgct tgttcagcag cttcacgctt agcttgttct gcggcctcac
    28201 gttcagcttt ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct
    28261 cttccgcttc acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc
    28321 gtttcacaaa ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg
    28381 taccaggaat gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg
    28441 aatttttctg attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt
    28501 cagcagactt ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct
    28561 gtcgctctgc ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag
    28621 tttattacgc ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg
    28681 ctttttcgtc ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat
    28741 cttccagcgt acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt
    28801 caaggttcag cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg
    28861 caatggtggc cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa
    28921 ggccttcgat ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga
    28981 agccttcctc taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg
    29041 tgtcgatcgc tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca
    29101 cgttaagttc ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa
    29161 tcgcctgcgc caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca
    29221 ccacgataga agcaacgtct gccggtgcca ttgcgttaat cacaaactgc gccgggttat
    29281 catcccacag gacgatatcg atacgctcgc cgcccagttc agtagaaacc gcctgaacac
    29341 gcgcgccacg catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca
    29401 ccgcgatttt cgcacgagaa cccgggtcgc gagccgctgc tttaatttca atcacttctt
    29461 cgccgatttc tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag
    29521 tgacgaacag ctgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc
    29581 ggtcgccagg gcggaagttt tcacgcggca gcatatcttc gcgcaggatc acggcttcag
    29641 cgttgttgcc cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga
    29701 tgatttcacc ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca
    29761 ctttctgcac gataacctgt tttgccgtct gggtggtgat acggtcaaag gtaacagact
    29821 caatctgatc ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt
    29881 caagggtgat ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg
    29941 tgtcaaaatc accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt
    30001 ttttctttgt tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta
    30061 gcgccttttc attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct
    30121 tttcacctca tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca
    30181 gcgcgaacac ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt
    30241 tgataacgcc ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct
    30301 cttctccgac aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg
    30361 aggagacttc caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta
    30421 cctggtggct cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga
    30481 tgcgcagtgt ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg
    30541 cctcaactgg cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc
    30601 ccaagacata aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc
    30661 cgataaatcg gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt
    30721 tccagaagga ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa
    30781 aaggactcta agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat
    30841 gagcccgacg agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg
    30901 ttttgggtaa aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc
    30961 tgcaggcatt atagtgatcc atgccacatt ttgtcaacgt ttattgctaa tcacgtgaat
    31021 gaatatccag ttcactttca tttgttgaat acttttgcct tctcctgctc tcccttaagc
    31081 gcattatttt acaaaaaaca cactaaactc ttcctgtctc cgataaaaga tgattaaatg
    31141 aaaactcatt tattttgcat aaaaattcag tgagagcgga aatccaggct catcatcagt
    31201 taattaagca gggtgttatt ttatgacgac gattctcaag catctcccgg taggtcaacg
    31261 tattggtatc gctttttccg gcggtctgga caccagtgcc gcactgctgt ggatgcgaca
    31321 aaagggagcg gttccttatg catatactgc aaacctgggc cagccagacg aagaggatta
    31381 tgatgcgatc cctcgtcgtg ccatggaata cggcgcggag aacgcacgtc tgatcgactg
    31441 ccgcaaacaa ctggtggccg aaggtattgc cgctattcag tgtggcgcat ttcataacac
    31501 caccggtggc ctgacctatt tcaacacgac gccgctgggc cgcgccgtga ctggcaccat
    31561 gctggttgct gctatgaaag aagatggcgt gaatatctgg ggtgacggca gcacctataa
    31621 aggaaacgat atcgaacgtt tctatcgtta cggtctgctg accaatgctg aactgcagat
    31681 ttacaaaccg tggcttgata ctgactttat tgatgaactg ggcggtcgtc atgagatgtc
    31741 tgaatttatg attgcctgcg gtttcgacta caaaatgtct gtcgaaaaag cctactccac
    31801 agactccaac atgcttggtg caacgcatga agcgaaggat ctggaatacc tcaactccag
    31861 cgtcaaaatc gtcaacccga ttatgggcgt gaaattctgg gatgagagcg tgaaaatccc
    31921 ggcagaagaa gtcacagtac gctttgagca aggtcatccg gtggcactga acggtaaaac
    31981 ctttagcgac gacgtagaaa tgatgctgga agctaaccgc atcggcggtc gtcacggtct
    32041 gggcatgagc gaccagattg aaaaccgtat catcgaagcg aaaagccgtg gtatttacga
    32101 ggctccgggg atggcgctgc tgcacattgc gtatgaacgt ctgcttaccg gtattcacaa
    32161 cgaagacacc attgaacagt atcatgcaca tggtcgtcag ttgggccgtc tgctgtacca
    32221 gggacgttgg tttgattccc aggcgctgat gctgcgtgac tctctgcaac gctgggttgc
    32281 cagccagatc actggtgaag ttaccctgga gctgcgccgt gggaacgatt attcaatcct
    32341 gaataccgtc tcagagaacc tgacctacaa gccagagcgt ctgacgatgg aaaaaggcga
    32401 ctcggtgttc tcgccagatg atcgtattgg tcaattgacc atgcgtaacc tggatatcac
    32461 cgatacccgc gagaaacttt tcggttatgc caaaaccggc ctgctttcct cctctgccgc
    32521 ttcaggcgtg ccgcaggtgg ataatctgga aaacaaaggc cagtaattcg acatcaaccc
    32581 tgcccttcag gggtggggtt accttgctta tcctctgtaa actcctttct gagcatcaca
    32641 taatcgaaaa catcatggtt tgcatccaca acccgtgagt ctcctttata gtgtgcaatc
    32701 acttcctcaa gcgtttgcgg ctgttccggc tttgttaccc ccatccacgc attaataagg
    32761 taattattgt aagctgtcga aaagatgttg ttttccgttg tgcgatccac accatcccca
    32821 agaacggggc tataccagat aaacatcgga acatgatatg cctgctggct ggcctccctg
    32881 cctccatgaa aatagacgtt cttcttcgta gggtcacgtt ccaggccgtg gtcggcaaaa
    32941 tacatgaccg aggcgcggcg atcttttaat aattcaaaaa cctgacctag caaactatct
    33001 gtgtaatgaa tggagttgtc atagcaggca tcctgatcgt cctgcggttg aaacacggcg
    33061 ctggattgcg gataggcgct acaagccggt tcatggcttc catttaaatg aagaacaatc
    33121 agtttctttt gctgcgtatt ttgctgtaat gcttgcgata aatgcggcaa caacaattca
    33181 tcaaatcctc tgacataaac tgtttccatg gcacgcatgg cgatactggt aactgctgta
    33241 ccattctgcc gaaaagcgga ttgcgagctt agccagaaag tctgaaatcc tgcttgatta
    33301 gccatattaa taatgttgtc ggggtaatta tgaatgtcat ggctcaaaac agaatcagca
    33361 gttaaagaaa ggggaaccga cagcgcagtg taaggtgcac cgcttattgc ttgattaaac
    33421 agtttgatct gttttctttg tgcttcgact tgcggtgtcg tagagcgtgt atatccatac
    33481 aaagacatat tgtcgacacg tacagactcc cccacaatca acacgtaggt atcaatacct
    33541 gtatccctga ctgataattg aaaatacggc actgtgtttg caattgagag taatctttga
    33601 tgctctttcg ctgctaaagc aaaataattg agattgaaaa acggcgtata ggtagcaaat
    33661 cgcgacgcta gtatatatgg actgaacgca tttttatttt tggcatcttt ataagcaaat
    33721 tgacaagcgg aaaataaact tcccgaaatg acaatcagca ataatattcc tgtcactttt
    33781 tttgtcggca aggaaacatc atattttatt attacagcca aaaaaagaag ggataaaaag
    33841 gcaaaagcac atagataagg gctatacatt cccagcattt tggcaacttc atccggatca
    33901 ctctgcagca cactaatcgc aaatccatca ttgaaagttg taccaaaggt acaccatgaa
    33961 tagaggctaa tactcatgtc tgccgcagtc agtaacacaa atggtgcggc aacaagcagg
    34021 cgaagcggaa gggatgatat acgtttaaca accaggatgg tcagaacgaa gaacagcaat
    34081 cgctgaacca tatgcgctcc ggaagaggcg actaagttcg taataccaaa aacaataaca
    34141 ctaagataca acaaccaatg agatttaaaa cttctagcaa atttgttgaa tactgtcatc
    34201 cagaaatcca agttcacaac caatataaac aattatcaac aatgtgcaat tttatatcag
    34261 acatgttttt caactctgat ccatatcaga acgaaacact gttagttttt tctttttaaa
    34321 tgatgcggcc attgcgttct cagtaattaa ataacattat caatgcgtct ctaaacatta
    34381 cgcagaacgt agccatcagt agttatactg acaggagcag gatggtttat cgagaagtaa
    34441 acatatccgt attcgtaccg gaactggagt taacacaata aagtgccaat tatgtcagca
    34501 gtagggaaaa gtttataacg aaggcgtacc ggcaccatcg tttcaaggtg ccagctacga
    34561 gtaaagcaac tggacgagat acagatacct gacaaccatt cctcagacca ggaccaaaaa
    34621 cgaaaaaaga cgcttttcag cgtctctttt ctggaatttg gtaccgagga cgggacttga
    34681 acccgtaagc cctattgggc actaccacct caaggtagcg tgtctaccaa ttccaccacc
    34741 tcggcacgga aactactttt tagttcggga tatcgctggt cggcttagcc ggagcagctg
    34801 gctgagtttg ttcggttttc gccggcgcac tcaggttttc ccattcgcta cctttattgg
    34861 ttttgttgct attgatgtta cccagcacca gactgatgat gaagaataac gttgccagca
    34921 acgccgtcat gcgggtcatg aagttaccag aaccacttga accaaacagc gtagcggaag
    34981 cgcctgctcc gaaggaggct cccatatcag cgcctttacc ttgctgcagc atgatcagac
    35041 caacaaggcc aattgccaca ataaggaaaa ctaccaaaag agcttcgtac ataatcaacc
    35101 tgttccttgc ggagttgccg cgtaccaatg cttcaaccaa taaagcggga gtttttaatc
    35161 tttcccactg aagcgggtgt gaatactaac caaagcgaat gaccttcgca agggcaattt
    35221 tatcgcattg tatcaactgc ggaaaaaaac agcaaaaccg atgtgttcgc cgagaaaaaa
    35281 ggcgacaatc gccgcctttt tagccagtta tctaacgctt taaacggctt ttactgcatc
    35341 ggcgatgcgg tgtgcaaatt cagtcacctg cgcttcgtct tcgccttcca ccatcacgcg
    35401 aattaacggt tcggtgccgg atttacgcaa cagcacacgt ccacggctac ccagcgccgc
    35461 ttcaacttct gcggtcacag ctttgactga ttcatgctcc agcggatcgc cgctacctgc
    35521 ggtgtaacgc acgtttacca ggatttgcgg gaacattttc atgccgctgc aaaggtcgtg
    35581 caggctcata tggttacgcg ccatcgccgc cagcacctgc aaaccagcaa cgatgccgtc
    35641 accggtagtg gttttatcca gcaggatcac atgaccggaa ttctctgcac cgatacgcca
    35701 gcctttctcc tgcatttttt ccagtacgta gcggtcaccc actttcgcgc gcgcaaatgg
    35761 aattcctaac tgtttcaacg ccagttcaag ccccatgttg ctcatcaatg tacccacagc
    35821 gccaccacgc agctggccct gacgaagacc ttcacgcgcg atgatataca tgatctgatc
    35881 gccatcgact ttattgcctt catggtcaac cataatcacg cgatcgccgt cgccgtcgaa
    35941 ggcaataccg agatccgctt tttcagccag cacacgagcc tggagcgcgc gaacgtcggt
    36001 agcccccact tcggcattga tgtttacacc gttcggctca caaccgatag cgataacgtt
    36061 cgcccccagt tcgcgcagca cgttcggcgc gatgtgataa gtcgcaccgt ttgcacaatc
    36121 caccacaatc ttcagttcac taaggctaag ttcgttcggg aacgtggctt tgcaaaactc
    36181 gatatagcga cccgcagcat caacgatacg gctggcttta cccagttctg ccgaatcaac
    36241 gcagctgatc tccttttcca tttccgcttc gatggcctct tctaccgcat ccggcagttt
    36301 ggtgccgtcg atagagaaga atttaatgcc attatcgtag aacgggttat gcgatgcaga
    36361 tatcacaatt ccggcctctg cgcggaaggt acgcgtcaga taagccacgg ccggtgttgg
    36421 catcgggcca gtgaagagtg cggaaaggcc cgctgccgcc agacccgctt ccagtgctga
    36481 ctccagcata tagccagaaa tacgcgtatc tttaccaata ataatcttac gggagccgtg
    36541 gcgcgccagc actttacccg cggcccaacc cagcttaagc acaaaatcag gggtgatcgg
    36601 cgcatcccct acacgaccac gaatcccatc ggtaccgaaa tatttacgat tactcatagc
    36661 gtttgttttc ctttgcagac agagtggctt ccaccacccg catcgcttct acggtttctt
    36721 tgacgtcatg aacacgaatg atgtgcgcgc cttgcattgc ggcaatgacc gcacaggcca
    36781 gactaccgct caaacgctcg gacggcccca cgttcagcag ctgtccaatc atcgattttc
    36841 gtgacatacc caccaacagc ggcaagttga aatggtgaaa ttcagccagg cgcgccagta
    36901 atgaatagtt atgggagaga tttttaccga aaccgaatcc ggggtcgagc aacaatttct
    36961 cttttgcgat acccgcctgc tcgcaacgtg ctatttgctc aataaagtag cgattcactt
    37021 ctgcaaagac atcatcatac ttcggggctt cctgcatggt ttttggattt ccctgcatat
    37081 gcatcagaca aaccggtaaa ccggtttctg cagccgcctc cagagcgcca ggttcggaaa
    37141 gggagcggat atcgttaata atgtgagcgc caactttcgc tgactcacgg atgacttctg
    37201 gtttggatgt atcgaccgag atccagactt cgaagcgttg agcaattgcc tcaaccacag
    37261 gaataacacg ttgcaactct tcttcaacgc taacttccgc cgcccctggg cgcgtggact
    37321 cgccaccaac gtcaatgatc gtcgcgccag cgttgatcat cagattcgca tgtttcaccg
    37381 catctatcag cgagttatgc gtgccaccat ccgaaaagga atcaggcgtg acgttgagga
    37441 tccccattac gtgaggatgg ctaaggtcca gtgaagtacc ctgggcaaag agtttcatga
    37501 tgttatccct ggtatgaatt gataagaaaa aaccccggag cacgccccgg ggttttcggt
    37561 acaaatacag tcatctgatg cgggaactta cttgtcgcct aactgctctg acatggtgtt
    37621 acccgggttc ggcgtacgcg gttcatcaac cggacgtgga gcctttggac taccattgtc
    37681 gccagaattg ttagaagcgc ctggttcttc ccagcccgct ggcggacgta catcgcgacg
    37741 tgccatcagg tcatcaatct gcggtgcgtc gatagtctca tatttcatga gagcatcttt
    37801 catcgcatgc agaatatcca tattgtcggt cagaagctga cgcgcacgat tatagttacg
    37861 ctcaatcagt gctttcactt cctggtcgat gatacgtgca gtttcatcgg acatatgttt
    37921 cgctttcgct acgctacggc cgaggaacac ttcaccttct tcttccgcgt acagcagtgg
    37981 acccaatttc tcagagaagc cccactgagt caccatgtta cgtgccaggt tggtcgcaac
    38041 tttaatatcg ttggacgcac cggtagaaac atgttccggc ccgtagatga tctcttctgc
    38101 cagacgacca ccgtacagcg tagaaatctg gctttccagt ttctgacggc tggcgctgat
    38161 tgcgtcgccc tcaggcaaga agaaagtcac acccagcgca cgaccacgtg ggataatcgt
    38221 cactttgtgc accggatcgt gttccggcac caggcgaccg ataatcgcat gacccgcttc
    38281 gtggtaagcc gtcgattctt tctgcgcttc cgtcatcacc atggagcgac gttccgcacc
    38341 catcatgatt ttgtctttcg ctttctcgaa ctcaaccatc gacacaacgc gtttgttgcc
    38401 acgagcagcg aacagtgccg cttcgttcac caggttcgcc aggtcagcac cggagaaacc
    38461 aggagtacca cgggcaatga ttgccgcgtc gatatcgggt gccaatggta cgcgacgcat
    38521 gtgaaccttc aggatctgct cacgaccgcg aacatctggc aagccaacca caacctgacg
    38581 gtcgaaacgg ccaggacgca gcagcgctgg gtcgagaacg tccggacggt tagtcgcggc
    38641 gataacgatg ataccttcgt taccttcgaa gccatccatc tcaaccagca tctggttcag
    38701 agtctgttca cgttcatcgt gaccaccgcc cagaccagcg ccacgctggc ggcctacggc
    38761 gtcgatttca tcgataaaga tgatgcacgg tgccgctttc ttcgcctgtt cgaacatgtc
    38821 acgaacacgg gatgcaccca caccgacgaa catttctacg aagtcagaac cggagatagt
    38881 aaagaacgga actttcgctt cgcctgcaat cgctttcgcc agcagcgttt taccggtacc
    38941 cggaggaccg accatcagga cgcctttcgg gatcttaccg ccgagtttct ggaagcggct
    39001 cggctcgcgc agatactcaa ccagttcagc aacttcttct tttgcttcgt cgcagcccgc
    39061 aacgtcagca aaggtcgttt tgatctgatc ttccgtcagc atgcgcgctt tgctcttacc
    39121 aaacgacatg gcacctttgc caccgccgcc ctgcatttga cgcatgaaga agatccagac
    39181 accaatcagc aacagcatcg ggaaccagga gatgaagata gaagccagca ggcttggttc
    39241 ttcaggcggt tcaccgacaa ccttgacgtt cttggtcaac aggttatcca gtagtttcgg
    39301 atcctgaacc ggaatgtaag tggtataacg gttactatct ttcttggtaa cgttgatttc
    39361 acgtccgttg atacgcgctt cacgaacctg gtcgttattg acctcttgta ggaaggtaga
    39421 gtaatccacc ttacggccat tagactcgct gggcccaaag ctctggaata ctgacatcag
    39481 cacaacggca atgaccagcc agagtattag gtttttcgcc atgtcactca agggattaac
    39541 ctcttattac aactgtgtta aaaacagcgt caggatactc tatatccagc atctttcaaa
    39601 ctttcgtctg aaatctcccg gttagggttt acgcccggtc gctacaatat acacttcccg
    39661 cgaacgtgca cgagaagagt ccggcttacg aactttgacc ttcgtaaaca gggagcgaat
    39721 ttcccttaga tactcatcga aaccttcgcc ctggaacacc ttcactacaa aactgccacc
    39781 tggcgctaat acatcacgac acatttctag cgccagttcc accagataca tggcacgggg
    39841 gatatccacc gccggtgttc cgctcatgtt tggtgccata tcggacatga caacctggac
    39901 tttgctgtcg ccaacgcgct ccagcagtgc tttcatcacc agttcatcac gaaaatcgcc
    39961 ctgaagaaag tccacaccaa cgataggatc cataggtaaa agatcgcaag cgatgatgcg
    40021 gcctttgccg ccaatttggg tgaccacata ttgtgaccaa ccacccggag cagcaccaag
    40081 gtcgacaacc gtcattcccg gtttaaagag tttgtcactt tgctgtattt catcaagttt
    40141 aaaccaggca cgggaacgta accccttttt ctgtgcctgt tgaacatatt tatcgctaaa
    40201 gtgttcctga agccagcggc tggagctggc agaacgcttc ttacctgtca tttaactttc
    40261 ccatggggat aactcatcgt aaccaattgc gtaaattttt acgcgcctat ttggtgatat
    40321 atgggagatg gcggtagaat gacccgtttt caatcccaac gtaagcaaaa aatacgatga
    40381 atctgagtac taaacaaaaa cagcacctga aaggtctggc acatccgctc aagccagttg
    40441 ttctgcttgg cagtaatggt ttgaccgaag gggtgctggc cgagattgaa caagcgttag
    40501 agcaccatga actcatcaag gtgaaaatcg ccaccgaaga tcgcgaaact aaaaccttga
    40561 tcgtggaagc tatcgtgcgc gaaaccggcg cctgtaatgt acaggtcatc ggtaaaacgc
    40621 tggtgcttta tcgcccaact aaagaacgta aaatctcgct gccacgctaa gattatccta
    40681 aagttacaca catttgctgt gtaaaacgag gggttttccg caggcaggag agcaaaatgc
    40741 cacgctctgt tcgttgataa aaggccgcat agcggccttt ttcctttctt tacaatacat
    40801 caacatcttg agtattgggt aattcttaca ggtattccac cttaattact tcaaattcta
    40861 cttcgccgcc cggcgttttg atgaccacaa catcatcttc ttctttgccg atcaggccac
    40921 gagcaatagg tgagtttaca gaaatcaggt tttgtttaaa gtcagcttcg tcatcgccaa
    40981 cgatgcgata agtctgttct tcgtcagaat cgagattcag caccgttacg gtagcaccaa
    41041 aaataacgcg cccattgttg ggcattttgg tgacatcaat cacctgcgcg ttcgacagct
    41101 tggcttcgat gtctttaata cggccttcgc agaaaccctg ctgttcacga gctgcgtggt
    41161 attcggcgtt ttctttcagg tcgccatgct cacgcgcttc cgcgatagca gcaatgattt
    41221 caggacggcg cacagatttc agaaaatcca gctcttcgcg taatttttca gcgccgcgta
    41281 aggtcatcgg aatagcttgc atttgttata cctcttgaat attcctgatg gggcaagtct
    41341 tcacccaccc cggctgttcg gcctgcccgg cataactcct caccgggacc agaagcaaaa
    41401 aaataccgac ccgggtacaa gtcccaggtc agctacaatt cacattttga tagtcatttt
    41461 accctgaagt tcccgaaggg tcatcgttta ctttataggg cgttgcgccg tagtatgacg
    41521 gctcgattcc aggttgttag cgcgagatta tgcgattttc cagatttatc atcggattga
    41581 ccagctgtat agcgttcagt gttcaggccg caaatgttga tgagtacatt actcaactcc
    41641 ccgctggtgc caaccttgcc ctgatggtgc aaaaagtcgg cgcgtcggcc cccgctattg
    41701 attaccacag tcagcagatg gcgctgcccg ccagtaccca gaaagtgatt actgcgctgg
    41761 cggcgttgat tcaactcggc cccgattttc gtttcaccac gacgcttgaa accaaaggca
    41821 atgtggaaaa cggcgtactt aagggtgact tagtggcgcg atttggtgcc gatccgacgt
    41881 taaaacgtca agatattcgc aatatggtcg cgactttgaa aaaatctggc gtcaaccaaa
    41941 tcgatggcaa tgtgttgata gatacctcca ttttcgccag ccacgataaa gcccccggct
    42001 ggccatggaa tgacatgaca caatgcttta gcgctccgcc tgccgctgcc atagttgacc
    42061 gcaactgttt ctccgtctcg ctctacagtg ccccaaaacc tggtgatatg gcttttatac
    42121 gcgtggcatc ttattacccc gttacgatgt tcagccaggt acgcaccctc ccccgtggtt
    42181 ctgccgaagc gcaatactgc gaactggatg tcgtaccagg cgacctgaac cgctttacgc
    42241 tgacgggatg cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc gtgcaggatg
    42301 gagccagcta tgccggggca attctgaaag atgagttaaa acaggcaggt atcacctgga
    42361 gcggaacact gctgcgccag actcaggtta acgaacctgg aacggtagta gccagtaaac
    42421 agtcggcccc gttgcacgat ctgcttaaga ttatgctgaa aaagtcggac aacatgatcg
    42481 ccgatacggt tttccgcatg ataggccatg cgcgcttcaa tgtgcctgga acatggcggg
    42541 ccgggtcgga cgccgtgcgt cagatcctgc gccagcaagc cggtgtcgat attggaaaca
    42601 ccattattgc cgatggttca gggctttcgc ggcataacct gattgccccc gccaccatga
    42661 tgcaggtgct gcaatacatt gcccaacacg acaatgaact taactttatc tccatgctgc
    42721 cgctggcggg ctatgacggc tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg
    42781 atggaaaagt ctcagcgaaa accggttcgt tgcagggggt atataacctg gcgggattca
    42841 ttaccacagc gagcgggcaa cgaatggcgt ttgtacaata tctttctggc tatgcagtag
    42901 aaccggcgga tcagcgtaat cgccgtattc cgttagtgcg ttttgaaagc cgtttgtata
    42961 aagatattta tcagaacaat tagtcaaaaa gaaaccccgg cacatggccg gggttttgct
    43021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    43081 cagtcctcat catcctcttc agcaatctct tcaagctgct ggcgatgata atcatcccac
    43141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    43201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccactcgc cgcagagatc
    43261 agataatatt tatcttccca gcccagcgcc tcagcgatcg ctttcgcttt ctcttcggct
    43321 tctaccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    43381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    43441 ccgtcaatcg gatcgatatc aatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    43501 aggaagcgaa tgcccagacc tgcaccttcc gcagcgcctt caatcagtcc tggaatatcg
    43561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    43621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    43681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    43741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    43801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    43861 ccgtgtttgg tcatatcgcc catggtttca ccagtaccct ggtcgattac acgcgtacct
    43921 accggcactt taattgtgac gtctttaccg cgcttaccgg tacagtcgcg gcttgcgcca
    43981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    44041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    44101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    44161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    44221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    44281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    44341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    44401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    44461 gcttccgcca gggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    44521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcaatt
    44581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    44641 ttttgcgcca cgccataact cacccaaacc gtcgcagcac cgaccccaaa gataactccc
    44701 caggtgtaat cggttagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    44761 aggccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    44821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    44881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    44941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    45001 ctcttcttca ccgcaagaat ggcccccagg ccaatactcg ccatcaagaa acggtaaaac
    45061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    45121 cagcaaattg ctgtggtgag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    45181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    45241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    45301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    45361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    45421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    45481 ccgaaacgct taacgcccag gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    45541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    45601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    45661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    45721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    45781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    45841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    45901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    45961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    46021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat caccctatat
    46081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    46141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    46201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    46261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    46321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    46381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    46441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    46501 ccgcgcgttc cagatgatga ccagcctcgg ttcgctcaaa gtgctggaag tcatgtcaga
    46561 agccgtaaac gtcatcgcag agggtgaagt tctgcaactg atgaacgtta acgatccgga
    46621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    46681 tgccgcgcag tgttccggaa ttctggcggg ctgtacgccg aaggaagaga aaggcctgca
    46741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    46801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    46861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    46921 cgccatcgaa cagggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    46981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    47041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    47101 cgctgttcaa cgcgatcgtt aatcccctcc cctcatcccg cgcagtgcgc gggatgattc
    47161 cagtaaactc cataagaata cctattcagc gtaataatgc actgaaaaac cagtgtatac
    47221 atctttatat attctgaata ttcacactct ttacaggaac tttttagagc aatcggccat
    47281 caggagtata gtgatgctcg acagaagaag tgttctgaat gaaagcgact aacttaagga
    47341 gtgaggaaag atggaaagta atttcattga ctggcatccc gctgacatca ttgcgggttt
    47401 gcgcaagaag ggaacgtcaa tggcggcgga gtctcgcaga aatggtttga gttcctcaac
    47461 gctggcgaac gcattatcgc gcccatggcc gaaaggagag atgattattg cgaaggccct
    47521 gggaactgac ccctgggtta tctggccatc acgctaccat gatccgcaga cccatgagtt
    47581 tatcgacaga acgcagttga tgcgtagcta cactaaaccg aaaaaatgag tggtctggcg
    47641 gtagccccgc gaacggggct gccagctttc agacgattac tcgcctttca cacgctcaat
    47701 atttgcacct aaagcgcgca gtttgtcttc aatgcgttcg tagccacgat cgatgtgata
    47761 aatacgatca accaccgtcg tcccttccgc aatacagcca gccagcacca ggcttgctga
    47821 tgcacgcaga tcggttgcca taacctgtgc gccagaaagt ttttcaacac cgtgacaaat
    47881 aacggtattg ctttcgattt cggcgtgtgc gcccatacgg ctcagctctg gcacatgcat
    47941 aaagcggttt tcaaagaccg tttcggtgat gaatccggtc ccttcagcca ccaggttcaa
    48001 cagcgtgaac tgggcctgca tatcggtcgg gaatgccgga tgcggcgcgg tacgtacgtt
    48061 aacagccttc ggacgtttgc catgcatatc caggctaatc cagtcttcgc cgacttcgat
    48121 gtccgctcca gcgtcacgca gtttcgccag cacggcgtcg agagtatctg gctgcgcgtt
    48181 acggcagata attttgccgc gagaaatcgc cgccgccacc aggaaagtac cggtttcgat
    48241 acgatccggc agcacgcgat agacaccgcc gcctaaacgt tccacacctt cgatgacgat
    48301 acgatcggtg ccctgaccgc taattttcgc acccagcgta atcaggaagt tcgcggtatc
    48361 gacgatttcc ggttcacgcg ctgcattttc aataatcgtg gtgccttccg ccagggttgc
    48421 agcacacatg atggtcaccg ttgcgccaac gctgacttta tccatcacga tatgtgcgcc
    48481 tttcagacga ccatcgacgg aagctttaac gtaaccttct tccagtttga tggtcgcgcc
    48541 taattgttcg aggccagaaa tgtgtagatc aaccggacgc gcaccgatcg tacaaccgcc
    48601 aggtagtgaa acttgcccct gaccaaagcg cgctaccagc ggccccagtg cccagataga
    48661 agcacgcatg gttttaacca gatcgtaagg tgcgcagaat acattaacgt cgcgggcatc
    48721 aatatgcaca gaaccattac gttctacttt cgcacccagc tggcttagca gcttcattga
    48781 tgtatcgacg tctttcagtt tcgggacgtt ctggatctct accggttctt ccgccagtag
    48841 tgcggcaaaa aggataggca gagcagcatt tttagcgccg gaaattgtga cttcgccctg
    48901 gagcttcgtt ggcccctgaa cacgaaattt atccatttag tttgttctca gttaacaatt
    48961 catatccgct accggcgaat cgcccatagc tcaaaagccg ttcagtttgc gatcgcgcgc
    49021 ccactccgca ggggtatacg ctttgatcga cacagcatga atgcggttat ccgcaatata
    49081 ttccatcagc ggaccataga ccgtctgctg ttttttaacc cgactcatgc cgtcaaacaa
    49141 ctcacccacg gcaataacct gaaagtggct gccatcgccg gaaacgtgga cttcctggag
    49201 ggagagagcg ttcatcaaca cgctctgaat ttcattattt tccatgggat cttcaatcat
    49261 cagttaataa accagcgaaa catcttagag caaagttgcg ctggcataaa taagcaaaaa
    49321 gcctcgctga taaatcagac aaggctcgac ttgcaggcag gtttgccgga caggcggtta
    49381 acgccatatc cggcctgaaa aaatttaacg aggcagaaca tcagcaggca aattatacaa
    49441 tttcgccagg gtatacactt tgtcgtttac cccctgaagc gtcacgttgt tgccctgctt
    49501 tttcgccaga tcgataagat ggagcagcag tgccagtcca cccgtatcca cgcgggacac
    49561 acggctaaga tcgatgcagg taatcccctt caccgcttcc tcacgcattt cccaaagcgg
    49621 tagcagaacg tcctgatcca gctctccgga taacgccagc gtgtcacccg tctgcatcca
    49681 gctcagtgac tcgctcatta ttttttctct tccagagtga ttttctgttg agaaatcgat
    49741 ttcagttgcg cagtcaggcc gtcgatacct ttggtacgca gcagcgttcc ccactcgttt
    49801 tgtttggtag tgatcatact gacgccttca gcaatcatgt cgtaagcctg ccagttgccc
    49861 gtctgggagt ttttacgcca ctggaagtcc agacgcaccg gcgggcggcc attcgggtca
    49921 ataatggtaa cgcgaatagg cacaatggtt ttatcgccca gcggctgttc tggcgcaatc
    49981 tgataggttt gaccgtgata catcgccagc gcctgaccgt aagcctgctt caggtactca
    50041 cggaaagcgg caaagtaagc atcacgttga gctggggtcg cactcttgta atactggccc
    50101 agtaccagcg caccggcgta tttcacctgt acgtatggca gcagttcctg atcaacaatg
    50161 gtacgcagat aatccgggtt ggcccgaatt tgcggttgct cattcttcag gcgatcgaac
    50221 gttttctgcg ccgcctcgtc catcagctta tacggattgg tttggtctgc cgcggttgcc
    50281 gcactcagcg gtgcaatcac cagcaaagcg accatcatta aacgtttaaa catgcgtcgg
    50341 ttctcctgaa attatttcgt tgtacccaca ggttcagtgg tttcattatt acctggcgca
    50401 gcagctggcg catcgccact attcttattg tcatcgcctt tactaccgta aaggaactga
    50461 ccaatgagat cttccagcac catcgcagac ttggtgtcct gaattgtatc gccatccttc
    50521 aggatagcag tccccagttc cgggtcttca aaaccgacgt ttaatgccag atattgttct
    50581 cccagcaggc cggaagtacg aatgctcagc gaactggtat caggaatgtg gttataacgt
    50641 tgttcaattt ccagcgttac gcgtggcaga taggttttcg ggtccagcgt aatatccgcc
    50701 acccggccca caacaacacc accaatgctg accggagagc gcgctttcag gccgccaatg
    50761 ttatcgaacg tcgcataaag cgtgtaggtc ggttcagtac gtattgacgt cacgttcgcc
    50821 gccttcaggc aaacaaacag cgccgccagc agtgctgcta ataaaaagat acccacccaa
    50881 atttcatttt ttttcgtttg catgaactca attcccaaac atcaatgcgg tcagcacaaa
    50941 atccagcccc agaacagcca gagacgagtg gacaacggtg cgagtggttg cccggctaat
    51001 cccggcagac gtcgggatgg cgtcgtagcc gttaaacaac gaaatccacg tcaccgtgat
    51061 ggcgaacacc acgctcttaa tcagacagtt gaccagatcc atacgccagt cgacggcatt
    51121 ttgcattgcc gaccagaaga acccgctatc aatgcctttc caactgacgc cgaccagcga
    51181 tccgccccag atccccacgg cgacgaaaat aaccgtcaac agtggtaatg aaataacccc
    51241 agcccagaaa cggggagaaa taacccgacg cagcggatcc accgccatca tctccatact
    51301 ggagagttgc tctgtagcgc gcatcaggcc gatttctgcg gttaacgccg aaccagcacg
    51361 cccggcaaac aacagcgcgg caaccaccgg ccccagttca cgcagtagcg ataacgccac
    51421 cagcataccc agactggttt ccgcactata agtggtcaga accagataac cttgcagccc
    51481 caacaccatt ccgatgaaca cgccagaaac tacaataatc agcatcgaca ggacgccgac
    51541 attatagagc tggcgcacca gcagcggcgc atgtttacga aattccggtt tgccgaccag
    51601 cgcattgaat aacattaacc cggcccgccc gaacgttctc agggttttaa tccctttatg
    51661 tccgagcgac gccagcgcat ttaacagcat gagtggctta actccctggt aaaagatcag
    51721 cgtgataatc gccggcagga tagcggaacg gaacaggccc gtcagctatc ccgtccagaa
    51781 actgacgtac gcgcggatca ggattcgcct gcaacgcctt ggcactgcca tgagcgacaa
    51841 tttttttgtc cgccaggatc caggcgtgat ccgcaatact taacacttcc ggcacatcgt
    51901 gagaaaccac cacacaagtc acgcccagcg cgctgttcag ctcagaaatc agcttcacca
    51961 gtacgcccat ggtaatggga tcttgcccaa caaagggctc atcaaacatg atgagatccg
    52021 gctccagcgc aatcgcacgc gccagcgctg cacgccgcgc catcccaccg gaaagttcag
    52081 aaggcattag tttagccgct ccacgcagcc ccacggcctc cagcttcatc atcaccgtac
    52141 tatgcaacaa tggcgcggga agttgggtat gttcgcgcag tggataggcg acgttgtcaa
    52201 atacgttcat atcagtaaac aacgccccgg actgaaataa catgctcatc cgtttgcgca
    52261 ctgtatacag gcgcgaacga gacatcgccg gaatattctc accatcgaaa aggatctcac
    52321 catgatctgg tgcgatttgc ccgccaatca gacggagtag cgtcgtttta ccgatgcccg
    52381 atggccccat gatcgccgtg atcttccctc gcggcacggt cagggaaata ttatcgaaga
    52441 tgcagcgatt gccacgcgta aaactgacat cgcgcatatc gactaaattc gccacagact
    52501 gctccataat tcacccttcg tcttgcgttg attttctaag catggcgctc aatttaacct
    52561 tgaacccaac atatttacag aatattaccc gccgtggtta gcgaaagctg gcatttgttt
    52621 tactttttag ccgcataaag tcaaaattaa gaatccgtta cggctttctg aaaatcttca
    52681 gcggaccggc gagtatacct gaagaaagga cgttagatgc ttttagctac ggcactgtta
    52741 attgttggtt tacttttggt cgtttacagt gccgaccgcc tggtttttgc cgcgtctatt
    52801 ctttgccgaa cctttggcat cccgccgctg atcatcggca tgacggtggt cagtattggt
    52861 acatcgttac cagaaatcat cgtctcgctt gccgcgtctc tgcacgaaca acgcgattta
    52921 gccgttggta cagccctcgg ctcaaacatt atcaatatat tgctgatcct cggtctggct
    52981 gcgctggttc gtccttttac cgtccattct gatgttctac gccgtgaatt acccttaatg
    53041 ttgttggtca gcgtggtggc cggttccgta ctctatgacg gacaacttag tcgcagcgat
    53101 ggtatctttc tcctctttct ggctgtgcta tggctgctgt tcattgttaa acttgcacgt
    53161 caggctgaac gtcaggggac tgacagcctg accagagagc agctcgcaga gctgccgcgt
    53221 gacggcggat tgcccgtcgc gtttttatgg ctcggcattg cgcttatcat catgccagtg
    53281 gccacgcgga tggtggttga taacgccacg gtgctggcga attactttgc catcagcgag
    53341 ttgacgatgg gtctgacggc aattgctatc ggaaccagcc tgccggaact ggcaaccgca
    53401 atagcggggg ttcgcaaagg tgaaaacgac attgctgtcg gaaatatcat tggcgcaaac
    53461 atttttaata ttgtcatcgt gttgggttta cccgcgctga taacgccagg agagattgat
    53521 ccactggcgt acagtcgtga ctacagcgtg atgttgctgg tgagcattat ttttgcgttg
    53581 ctgtgctggc ggcgctcccc gcaaccgggc cgtggtgtag gggtattatt aactggcgga
    53641 tttatcgtat ggctggcgat gttgtactgg ttatcgccaa tactcgttga ataactggaa
    53701 acgcattatg tcgcacgtag agttacaacc gggttttgac tttcagcaag caggtaaaga
    53761 agtcctggcg attgaacgtg aatgcctggc ggagcttgat caatacatca atcagaattt
    53821 cacgcttgcc tgtgaaaaga tgttctggtg taaagggaaa gttgtcgtca tggggatggg
    53881 gaaatcgggg cacatcgggc gcaaaatggc cgcaacgttt gccagcaccg gtacaccttc
    53941 atttttcgtc catcctggtg aagccgcgca tggtgattta ggcatggtca ccccacagga
    54001 tgtggtgatt gctatctcta actcaggtga atccagcgaa atcactgcct taattccagt
    54061 gcttaagcgt cttcacgtac cgttaatctg catcaccggt cgcccggaga gcagcatggc
    54121 gcgcgccgca gatgtgcatc tgtgtgttaa agtagcgaaa gaagcctgtc cgttagggct
    54181 ggcaccgacc agcagcacca ccgccacgct ggttatgggc gatgccctcg ctgtcgcgct
    54241 gttaaaagca cgcggcttta ctgctgaaga ttttgcgctc tcacacccag gcggcgcact
    54301 gggtcgtaaa cttctgctgc gcgtaaacga tattatgcat acgggcgatg agatcccgca
    54361 tgttaagaaa acggccagtc tgcgtgacgc attgctggaa gttacccgca aaaatcttgg
    54421 tatgactgtc atttgcgatg acaatatgat gattgaaggc atctttaccg acggtgattt
    54481 acgccgtgtc ttcgatatgg gcgtggatgt tcgtcagtta agtattgccg atgtgatgac
    54541 gccgggggga atacgcgtgc gccctggcat tctggccgtt gaggcactga acttaatgca
    54601 gtcccgccat atcacctccg tgatggttgc cgatggcgac catttactcg gtgtgttaca
    54661 tatgcatgat ttactgcgtg caggcgtagt gtaaagattc aaggataaac aacaatgagc
    54721 aaagcaggtg cgtcgcttgc gacctgttac ggccctgtca gcgccgacgt tatagcaaaa
    54781 gcagagaaca ttcgtctgct aatcctcgac gttgatggcg tactgtcaga tggcctgatt
    54841 tatatgggca ataacggcga agagctgaaa gcgttcaatg ttcgtgacgg ttatggcatt
    54901 cgttgtgcgc tcacctctga tattgaagtc gctatcatta ccgggcgaaa ggctaaactg
    54961 gtagaagatc gttgtgccac attggggatc actcacttgt atcaggggca gtcaaacaaa
    55021 ctgatcgcct ttagcgatct gctggaaaaa ttggcgattg ccccggaaaa tgtggcttat
    55081 gttggcgatg atctcatcga ctggccggta atggaaaaag tgggtttaag cgtcgccgtg
    55141 gccgatgcgc atccactgtt gatcccgcgc gccgattacg tgacgcgcat tgctggcggt
    55201 cgtggcgcag tgcgcgaagt ttgcgactta ttactcctgg cgcagggcaa actggatgaa
    55261 gccaaagggc aatcgatatg agtaaagcca gacgttgggt tatcattgtg ctatcactgg
    55321 cggttctggt gatgatcggc attaatatgg ccgaaaaaga cgataccgcc caggtggtcg
    55381 tcaacaacaa tgatcccacc tataaaagcg agcatacgga cacgctcgtc tataacccag
    55441 aaggggcact aagctatcga ttgattgctc aacacgttga atattattcc gatcaggccg
    55501 tttcgtggtt tacgcagccg gtacttacca cgtttgataa ggataaaatc ccgacatggt
    55561 ccgtaaaagc agataaagcc aagctgacca atgaccggat gctctattta tatggacacg
    55621 ttgaagtcaa cgcactcgtg ccggactctc aacttcgcag aatcacgacg gataacgcgc
    55681 agatcaattt ggtgacgcag gatgttacct ctgaagacct cgtcacgtta tacggaacaa
    55741 catttaactc cagcggtctg aaaatgcgcg gcaacttacg cagcaagaac gccgagctga
    55801 ttgaaaaggt tagaacatcc tatgaaattc aaaacaaaca aactcagcct taatcttgtg
    55861 cttgccagct cacttctggc cgccagcatt ccggcatttg ccgtaaccgg agacactgat
    55921 cagccgatcc acattgaatc ggaccagcaa tctcttgata tgcaaggcaa cgtggttacc
    55981 tttaccggta atgtcatcgt cacccagggc accatcaaaa ttaatgccga caaagtggtc
    56041 gttacccgtc cgggcggcga acaaggtaaa gaagtgattg acggctacgg taaaccggca
    56101 acgttctacc agatgcagga caacggtaaa cccgttgaag gtcacgcttc ccagatgcac
    56161 tacgaactgg caaaagattt tgtcgttctg acgggtaatg cttatctgca gcaggtcgat
    56221 agcaacatta agggcgataa gatcacttac ctggtgaaag agcagaaaat gcaggctttc
    56281 agcgacaaag gcaagcgcgt aaccaccgtt ctggtaccgt cgcagctgca ggacaaaaac
    56341 aacaaaggcc agaccccggc acagaagaag ggtaattaat tcgttatggc aacattaact
    56401 gcaaagaacc ttgcaaaagc ctataaaggc cgtcgcgtgg tagaagacgt cagcctgacc
    56461 gtcaactccg gggaaattgt cggtctgctg gggccaaacg gtgccggtaa gaccaccact
    56521 ttctacatgg ttgttggcat tgtgccgcgc gatgcgggca acatcattat tgatgatgac
    56581 gatatcagtc tgctgcctct gcatgcacgc gcgcgccgcg gtatcggcta tctgccacag
    56641 gaagcctcca ttttccgtcg cctcagcgtt tacgataacc tgatggcggt actgcaaatt
    56701 cgtgacgact tgtctgctga acaacgtgaa gaccgtgcga acgagctgat ggaagagttt
    56761 cacattgagc acctgcgtga cagcatgggg cagtcactct ccgggggtga acgtcgccgc
    56821 gtagaaattg cccgcgcact ggctgcgaat ccgaaattta ttctgctcga cgaaccgttt
    56881 gccggggttg acccgatctc ggttatcgac attaaacgca tcattgagca cctgcgcgac
    56941 agcggcctgg gcgtgctgat cactgaccac aacgtgcgtg aaacactggc ggtttgtgaa
    57001 cgcgcttata tcgtcagtca ggggcatttg atcgcccacg gcacgcctac agaaatctta
    57061 caagacgaac acgttaagcg tgtatacctt ggggaagact tcagactctg atagggtaga
    57121 agtttgcgat gttttagcag gagagtacga ttctgaacat gaagcaaggt ttgcaactca
    57181 ggcttagcca acaactggcg atgacgccac agctccaaca ggcaattcgt ctgttgcagt
    57241 tgtcgacgct ggaacttcag caggagctac agcaggcgct ggagagtaat ccgctgcttg
    57301 agcaaatcga cactcatgaa gaaatcgaca cccgcgaaac gcaagacagt gaaacgctgg
    57361 acaccgccga cgcgctcgaa caaaaagaga tgccggaaga gctgccgctc gatgccagtt
    57421 gggacaccat ttacaccgct ggtacaccat ccggcaccag cggtgactac attgacgacg
    57481 agctaccggt ctatcagggc gaaacgacgc agaccttgca ggattacctg atgtggcagg
    57541 tcgagctgac accgttttcc gacactgacc gcgctattgc tacctctatc gtcgatgccg
    57601 ttgatgacac cggttatctg actgtcccgc tggaagatat tctcgaaagt atgggcgatg
    57661 aagagatcga catcgacgaa gttgaagccg tccttaagcg gatccaacgg tttgatccgg
    57721 tcggtgtggc ggcaaaagat ctgcgtgact gcctgctgat ccaactctcc caattcgata
    57781 aaaccacgcc gtggctggaa gaggccagac tgatcattag cgatcatctc gatctgttag
    57841 ccaatcacga cttccgcact ttaatgcgcg tcacgcgtct gaaagaagat gtgctgaaag
    57901 aagccgtcaa tctgatccag tcgctcgatc cgcgccccgg gcagtcgatc cagactggcg
    57961 aacctgagta tgtcattcca gatgtgctgg tgcgtaagca taacggtcac tggacggtag
    58021 aactcaacag tgacagcatt ccgcgtctgc aaatcaacca gcactacgcc tcgatgtgca
    58081 ataacgcgcg caacgatggt gacagccagt ttatccgcag caatctgcag gatgccaaat
    58141 ggttgatcaa gagtctggaa agccgtaacg atacgctact gcgcgtgagt cgctgtatcg
    58201 ttgaacagca gcaagccttc tttgagcaag gcgaagaata tatgaaaccg atggtactgg
    58261 ccgatatcgc ccaggctgtc gaaatgcatg aatcgacgat atctcgcgtg accacgcaaa
    58321 aatacctgca tagtccacga ggcatttttg aactgaagta tttcttttcc agtcacgtca
    58381 ataccgaggg cggcggcgaa gcttcctcca cggcgattcg tgcgctggtg aagaaattaa
    58441 tcgcggcgga aaacccagcg aaaccgttga gcgacagcaa gttaacctct ttgctgtcgg
    58501 aacaaggtat catggtggca cgccgcactg ttgcgaagta ccgagagtct ttatccattc
    58561 cgccgtcaaa ccagcgtaaa cagctcgttt gacccaaccg ataaggaaga cactatgcag
    58621 ctcaacatta ccggaaataa cgtcgagatc accgaggcac tgcgcgaatt tgttacagcc
    58681 aaatttgcca aacttgagca atattttgac cgaatcaacc aggtctatgt tgttctgaaa
    58741 gtggaaaaag tcacccacac ctcagatgca acactgcatg taaacggtgg tgaaattcat
    58801 gccagcgcgg aaggtcagga catgtacgct gccattgatg gcttaattga taagctggca
    58861 cgccagttga ccaaacataa agataaactg aaacaacact aattgtccgg gcaattagca
    58921 tgtgcatggc ggcctgttgt gcggcacaac gggccatttg tacggttaat gctccgagcc
    58981 tgttccactg tttgagtggg caggttctta ggtgaaatta tgacaaataa tgatacaact
    59041 ctacagctta gcagtgttct taacagggaa tgtacgcgaa gccgcgtcca ctgtcagagc
    59101 aaaaaacgcg ccctggaaat catcagcgag ctggcggcga aacaacttag cctgccgcct
    59161 caggtggttt ttgaagctat cctgacgcgg gaaaaaatgg gcagtaccgg tatcggcaat
    59221 ggtattgcca ttccgcatgg caaactggaa gaagatactc tgcgtgccgt tggcgttttc
    59281 gttcagctcg aaacgcctat agctttcgat gccatcgaca accaaccggt ggacttactt
    59341 tttgccctgc tggtgccggc agaccaaact aaaacgcacc tgcatactct gtcgctggtg
    59401 gcgaaacgtc tggcggacaa aaccatctgc cgccgtttac gcgcagccca gagcgatgaa
    59461 gagctgtatc aaatcattac ggataccgaa ggtactccgg atgaagcgta gttattcggt
    59521 aatgtctctt ttagacgttg tgaggagaaa cagtacatgg tactgatgat cgtcagcgga
    59581 cgttcaggtt caggtaaatc tgtcgccctg cgtgcgctgg aagatatggg tttttactgc
    59641 gtggataacc ttcccgtagt gttgttaccc gatctggctc gaaccctggc cgatcgcgag
    59701 atttctgccg ccgtcagcat tgatgttcgt aatatgccgg agtcaccaga aatattcgaa
    59761 caggcgatga gtaacctgcc tgacgctttc tcaccgcaac tcctgttcct ggatgccgac
    59821 cgtaatacct taattcgtcg ttacagtgac acgcgccgac tgcatccgct ttccagcaaa
    59881 aacctgtcgc tggaaagtgc tatcgacaaa gaaagcgatc tgctggagcc tctgcgttcg
    59941 cgagcggatc tgattgtcga tacctcagaa atgtccgttc acgagctggc agaaatgctg
    60001 cgtacccgtc tgctgggtaa acgtgaacgc gaactgacaa tggtctttga gtctttcggc
    60061 ttcaaacacg gtatccctat cgatgcagat tacgtctttg acgtgcgctt cttgccgaac
    60121 ccgcactggg atccgaaact gcgtccaatg acaggtcttg ataagcctgt cgccgcgttc
    60181 ctcgaccgcc acacagaagt acacaatttt atctaccaga cgcgaagcta tcttgagcta
    60241 tggttaccta tgctggaaac caacaaccgt agctacctga cggtcgccat tggttgtacc
    60301 ggcgggaagc accgttcggt gtatattgca gagcaactgg ctgactactt ccgctcgcgc
    60361 ggtaaaaacg tccagtcacg ccatcgtacg ctggaaaaac gtaaaccatg accgtcaagc
    60421 aaactgttga aatcacaaac aagctgggca tgcatgcccg gcctgcaatg aagctgtttg
    60481 aattaatgca gggttttgac gctgaagtgc tgttacgtaa tgacgaaggc accgaggctg
    60541 aagccaacag cgttattgcg ctgctgatgt tggattctgc caaaggacgg cagattgaag
    60601 ttgaagcgac cggtccacag gaagaggaag cactggccgc cgttatcgcc ctctttaatt
    60661 ctggttttga tgaagattaa tcttcatcac tttgacatac aactcccttc aaactccccc
    60721 cgacaataag aaaatcacgt actgaaatcg ttctcaatca acgtcaattg tacatttcgt
    60781 gcgcttttta ctttcagaag aaccttaaga aaaccttaag aagcattgtt taggtttcgt
    60841 ttaagttaat cgaccatact ggagatcgtc agaaaatatt tccaggagat ggcatgattc
    60901 gcttatctga acaaagtccc ctgggcaccg gaagacatcg gaaatgttat gcgcatccgg
    60961 aagatgccca acgctgtatc aagattgtct accatcgtgg cgatggtggc gataaagaga
    61021 tccgccgtga gttaaagtac tacgcgcatc ttggtcgccg gttaaaagac tggagtggaa
    61081 taccgcgcta tcacggtacc gttgagacgg attgcggaac cggttatgtc tacgatgtga
    61141 ttgccgattt tgacggcaag ccctccatta cgctgaccga atttgctgaa caatgccgtt
    61201 acgaagaaga cgttgcacaa ctgcgccaat tactgaaaca gctgaagcgt tatttgcagg
    61261 ataaccgtat cgtgacgatg tcgctgaagc cgcagaatat cctctgtcac cgcattagtg
    61321 agtcagaggt tatccccgtc gtctgcgaca acattggtga aagcacattg attccactgg
    61381 cgacctggtc aaagtggtgt tgtctgcgta agcaagagcg gttgtggaaa cgatttatcg
    61441 cccaaccggc gctggctatt gccctgcaga aagatctgca acctcgtgag agcaaaacgc
    61501 tggccctcac ttcgcgcgaa gcttaatcca gctggtgttg ttgcataaac ggctcaccgc
    61561 ctaactgata catctgccgt aaaatccacg cctgacggct gcgcacgtag cccgatggtg
    61621 cggagacttt gaaacgaagt ggattaggta atacagctgc cagtaaggca gcttccgatc
    61681 gggtaagttt gctcgcgggt ttgtggaaat agcgttgtgc cgcagcttcg acgccaaaca
    61741 caccgtcgcc aaattcggcg atattcaggt aaaccgtcag gatacgcttt ttgctccaga
    61801 ctgtttctat ccccagcgtt aatccggctt ccagcccttt tcgaacccag ctacgcccat
    61861 cccataaaaa gagatttttg gccgtctgct gagaaatcgt tgaagcaccg cgaatacggt
    61921 tttcattgcg ctcgttgtgc gccagggctt tctcaatgga agcgacatca aagccccagt
    61981 gctcaggaaa tttctgatct tctgcggcaa tcaccgccag tcccatccac ggcgagattt
    62041 gatccatact gacccagtca gaatgtgcca cgtaacgaaa attgccatgc agccaggcgc
    62101 tgacctgtcg ctcgaccatt accgctgaga aaggaacagg cgcaacgcta aacaacgcga
    62161 tgcccccgcc ccagaaaacg gcgaggacaa ccattaaccg cagaagaaaa cggcgaacga
    62221 aactaaacac cgttaagcgg cttttactca ttcagccaga accagcacgc gggaaaccag
    62281 cttatcaatg ccgctcgccg cttctgcaat gttctgcgcc agcatatatg ccggggtggt
    62341 gacaatctta ttgtcttcat caaccacgat atcatcgaca ggacacggca catgctctgc
    62401 gcccatctct tccagcactt ctgcggtatc gatatcagta ccgatggtca aacgcagcgg
    62461 gaaatcgaaa attttcggca gcatcgccgg ggcaatacac ataaaaccaa gcggttttcc
    62521 ggcctgatgc atcgcttgtg ccagcgcctt taattcacgg tcaacggtgc attcgcgacc
    62581 aagactggca aaattgctta aattctttgc cgcgccaaac ccccccggca caatcaacgc
    62641 atccagttca gcggcatcgg cctgggccag aggacggatt tcaccgcgcg ttattcgtgc
    62701 cgcttcaatc agcacattgc gcgtttccgt catcgcttcg ccagttaaat ggttgataac
    62761 atcaacctgc tgcttatccg gtgcaaagca gaccgcctgc gcaccgctgc gtgaaatagc
    62821 taacaacgtc aacaccgctt catgaatttc agaaccgtca tagacaccgc atccgctcag
    62881 aattacgcca attttcttca ttgtgatcat cctttcgcaa ttgactgaaa cacattaatt
    62941 tttttaataa aaatggtacg catcacacat ttaactgatt catgtaacaa atcatttaag
    63001 ttttgctatc ttaactgcgt gcggcctgaa aaacagtgct gtgcccttgt aactcatcat
    63061 aataatttac ggcgcagcca agatttccct ggtgttggcg cagtattcgc gcaccccggt
    63121 ctagccgggg tcatttttta gtggcttttg ccacccacgc tttcagcact tctacgtcgt
    63181 gacgccactc ttctttcatc tcttcaatcc attcaccgac gttatcttcc caggcaggaa
    63241 ggtcaggaga ctgaatttgc tgacccagct gttgcagatg gcgtaacccc actgaccccg
    63301 ccgcaccttt aattttatgt ccttcctcaa caatgccttt tttatcctgc gccgtcagat
    63361 tcgactccag cacgctgaca tagcctggca tcatcttctc aaacaccgct aacccgtcgg
    63421 tgatcagctt cggtcctaca agttcgagat actgttccag catgggaata tcgagcaatg
    63481 cttctgattt actgttctct tctgtcgtca ccgtactctc ctcatcatcc tgggtatccc
    63541 agaatttctt gatcatcgcg gttagcgccg gaacagaaag cggcttactc agcacatcat
    63601 ccattccagc attgaggtac tcttgtttgt ctttcaacac gttagcggtt aaggccacca
    63661 gcggcggtaa atcctcgcgc ggataacgct tcgtcagttc acgagagata tccagcccgg
    63721 tcatatctgg caactgaata tccagcaaca ccaggtcgta ttcgcccggt ttaaacatct
    63781 ccagcgccgc cttgccggtc atggcgacat caacgctgtt acctaatttt tccagcacag
    63841 aacgcgcgac aatcacgttc agttcaatgt cttccaccag cagcacattc agcgccggta
    63901 aaggcatatc gtcttcatca aacgcatcat cgacctcttc tgctaccgac ggcgcgtgga
    63961 tcgtcaacgt aaaggttgaa cctttaccct gttcgctggt aaccgtaata tcgccgccca
    64021 tatttttcgc cagacgacga gaaacggcca gaccaatacc ggtgccggtg gcaggtttac
    64081 cgccatgact gtctttcacc tgataataca tggcgaagat tttatccagc tcatcctgcg
    64141 gaatgccgat accagaatct tccacttcaa aatgcagcat atcgccttca tcgtagcgca
    64201 cgcgcacggt aacctggcct tgctgggtga atttgacggc gttactgatg aggttccaca
    64261 ggatctgccg taaacgcgtc ccgtcggtaa tgacctgatg cggtaatggc agcgtcggct
    64321 ccagattaaa gcgcaatcct ttttgttgcg cctgcaaggc ggagagattt tccagatcgg
    64381 caaggaagct ggtgaaatca accggctgat tatcaagctg gaccttgcgc cgttccatct
    64441 tatccatgtc gataatatcg ttgaagatat tccccagcgt gacggccgaa acatggatag
    64501 ttttgagata tttttcctgc tcggcggtga gttcggtatc cagtaggatg cggctcaggc
    64561 cgacgatacc gttcagcggt gtacgcaatt cgtgactgat ggtggaaata aacgtcgttt
    64621 tgtcgcggct ggcgcgttca agcgcatcct gataccgctt acgctcggta atgtcgcgac
    64681 caaagcccat caaaccgtga cgtttaccca cgcggtcgta gtacggcact ttacgaattt
    64741 caaagcaggc tttgcgtccg tcgggataat ccagccactg ctcataggtc agtgacacat
    64801 tatgacggaa cactttttca tcggtttcaa tgactttcgc agcggcttcc ggtgagtaaa
    64861 catcagcagg tttcagatga accagttgtt tttcgctttt tccggtcagc agctccatcg
    64921 cgcggttaca gccggaaaac tctttatctt cgttacgata aaaaaccagg tcgggtgaag
    64981 catcaaggaa ggaacgtaag aatgaggatt gctgctcgag ctgaatttgt gtctcttcgc
    65041 gctctttgat ttcaattttc agttggccga aggtttcctg cagttctgct tccgcttttt
    65101 cacgaacggc aatttcctga tttagctggg caatattatc ttttaactga acgttgaggc
    65161 tcaaatcgcg ctcgcgcatc tcctccagtt tttgcaccag ccgtgacaga cgttgtcgtg
    65221 actcctccag ttgctcgacg accaccgata gaaagtagac cgcccacggc gtaatcagca
    65281 aaccaaagaa gatagaacga ataacatcaa tgctttcgac ctgaccatgc agcaccatgg
    65341 ttaccgccat ttgtaccaca atggcaagaa cgacgagggc cagcgccagc aacattgaga
    65401 agcgcaccag acctaacttc atcatcaggt caacataata ctgcgccagc agacgaattt
    65461 gcttcattag ggaattcctt cacgacaacc tgccccaata atacccaatt ctgacagtta
    65521 cgttataaat tgtgtgagaa atgcggaatt cccctcaatg acctactccg taggaggtag
    65581 ccacggacgt cccagcgccg atccctgtac gccatgttca ttcagatagc gatccagctc
    65641 gaccattccc gtccatcgat tttcgcacca cagcggagca agcagcgtcg ggcgacgggc
    65701 gctggcggaa atgcggtggt agatcacttc cggtggcgta tggcgaatca tttctccggc
    65761 agtgagtgtg taatcctcca gttcaatacc gttcaaacgc cccgcttccc aggctttcgc
    65821 cataatgctg cctttcacaa tatgcagcgg atgcagcttt atgccgtcca cgccggtttc
    65881 aaccacgcgc tccagcgttt gcaaacattc agcctgccct tcgccaggca ggccgacaat
    65941 taagtgggaa cacaccttca gcccgcgctg acgtgccagc tgggttgtac gctgataaca
    66001 ggcaaaatca tggccgcggt tgatgcgatg cagtgttttg tcgtgggcgg tttgtagccc
    66061 cagctccagc cacacttcgt aaccctggtc cttatattcg caaagcagat ccagcaccgc
    66121 atccggcacg cagtccgggc gggtaccaac acacaaaccg acaatattgg cctggctcac
    66181 cgcctgctga tacatagaac gcagcacctg aacttccgca aaggtgctgg tatacgcctg
    66241 aaagtaggcc agatagcgtt tagcgcggtt aactaaattc gcctggtgcg ccagttgctc
    66301 ggcaatggaa cgatgctgct gcgcttcatc ggcaaacgag gccacattac agaatgtgca
    66361 gccaccacgc ccgatggtac cgtcacggtt agggcagcta aaaccgccgt gcagcgtcag
    66421 cttatgcacc ttttgcccat aacgacgggt gagatcacca ccaaacatat tgactaattt
    66481 ctgtaactgc ataatctgat agaccgcgcc ttgaaaagag gccaaagcct gccattttta
    66541 gctcaattcg gcgatgacct ggatcaatcg tcccggcctg cttttatcaa ctgcataatc
    66601 aatcaaaatt accgaaattt catgcataat cacataaatc acttttgctt atcttgtgtc
    66661 agattttttt atctccttat ggattttagg caaaaacagt agcatgaaac gccattacca
    66721 attaaggcag tataaaatgc tggttttctc gtcagttcaa ggcaggataa gggttaacac
    66781 gcctttatga cagtcaggaa ttggctgttt ctctaacgac gctcctttta gccttaatta
    66841 gaaaatctat tttaatttca gtcaattaat aaagaatttt gcgctaaagc acatttctgt
    66901 accaataagc ttgccatttg acctgtatca gctttcccga taagttggaa atccgctgga
    66961 agctttctgg atgagcagcc tgctcatcat atttatgcag taattgagat cccctcttca
    67021 ccgtattaac cgatgcgaaa aggacaacaa gggggcgaat gcgaggcgcg cgtatgacac
    67081 gcaaaccccg tcgccacgct ctttctgtgc ccgtgcgcag cggttcggaa gtggggttcc
    67141 cgcagagcct gggggaggtt cacgatatgt tgtacgataa atcccttgag agggataact
    67201 gtggtttcgg cctgatcgcc cacatagaag gcgaacctag ccacaaggta gtgcgtactg
    67261 caatacacgc actggcccgc atgcagcacc gtggcgcgat tctcgccgat ggtaaaaccg
    67321 gtgacggttg cggcttgctg ttacaaaaac cggatcgctt ttttcgcatc gttgcgcagg
    67381 agcgcggctg gcgtttagcg aaaaactacg ccgtcgggat gctattcctg aataaagatc
    67441 ctgaactcgc cgctgccgca cgccgcatcg ttgaagaaga actgcaacgc gaaaccttgt
    67501 cgattgtggg ctggcgtgat gtccccacta acgaaggcgt gctgggtgaa atcgccctct
    67561 cctctctgcc acgcattgag caaatttttg tgaacgcccc ggcaggctgg cgtccgcgtg
    67621 atatggaacg ccgtctgttt atcgcccgcc gccgcatcga aaagcgtctc gaagccgaca
    67681 aagacttcta cgtctgtagc ctgtcgaatc tggtcaatat ttataaaggt ctgtgtatgc
    67741 cggcggatct gccgcgcttt tacctggatc ttgcggacct gcgcctggaa tcggccatct
    67801 gcctgttcca ccagcgcttc tccactaaca ccgtaccgcg ctggccgctg gcgcagccgt
    67861 tccgctatct ggcgcataac ggtgaaatca acaccatcac cggtaaccgc caatgggcgc
    67921 gtgcgcgtac ctataaattc cagacaccgc ttatccctga cctgcacgac gccgcaccgt
    67981 tcgtcaacga aaccggctct gactccagtt cgatggataa catgctggaa ctgctgctgg
    68041 caggcgggat ggatatcatc cgcgccatgc gtctattagt accacccgcc tggcagaaca
    68101 acccggatat ggacccggaa ctgcgtgcct tctttgactt taactccatg catatggagc
    68161 cgtgggatgg cccggcgggc atcgtgatgt ctgacggtcg ttttgctgcc tgtaacctcg
    68221 accgtaacgg tctgcgtccg gcgcgctacg tcatcaccaa agataagctc atcacctgcg
    68281 cctctgaagt cggtatctgg gattaccagc ctgacgaagt ggtcgaaaaa ggccgcgtcg
    68341 ggccaggcga actgatggtg atcgacactc gcagtgggcg tattctgcac tcggcagaaa
    68401 ccgatgacga tctgaaaagc cgccaccctt ataaagagtg gatggagaaa aacgtccgcc
    68461 gactggtacc gtttgaagat ctgcccgatg aagaagtggg tagccgcgaa ctggacgacg
    68521 acacgcttgc cagctaccag aaacagttta actacagcgc ggaagagctg gactccgtaa
    68581 ttcgcgtact gggcgaaaac ggtcaggaag cggtcggttc gatgggcgat gataccccat
    68641 tcgccgtgct ctccagtcag ccgcgcatta tttacgacta cttccgccag cagtttgccc
    68701 aggtgactaa cccgccaatc gacccgctgc gtgaagcgca tgttatgtcg ctcgccacca
    68761 gcatcggtcg cgaaatgaac gtcttctgtg aagcagaggg ccaggcgcac cgtttaagct
    68821 ttaaatcgcc gattctgctc tactccgatt tcaaacagct cacgacgatg aaagaggagc
    68881 actaccgcgc agatactctg gatatcacct ttgacgtcac taaaaccacg ctcgaagcga
    68941 cggtcaaaga gctgtgcgac aaagccgaaa aaatggtccg tagcggcact gtgctgctgg
    69001 tgctctccga ccggaatatc gctaaagatc gcctgccggt tccggccccg atggcggttg
    69061 gcgcgatcca aactcgtctg gtcgatcaaa gcctgcgttg cgatgccaac atcatcgtcg
    69121 aaactgccag cgcccgcgat ccgcaccact tcgccgtgtt gctgggcttc ggcgcgacgg
    69181 ctatttatcc gtaccttgcc tatgaaacgc tgggccgcct ggtagacact caggcgattg
    69241 ccaaaaatta tcgtacggtg atgctgaact accgtaacgg catcaacaaa ggcttgtaca
    69301 aaatcatgtc caaaatgggc atctccacca tcgcctctta tcgctgctcg aaactgtttg
    69361 aagcggtcgg tctgcacgat gatgtagtgg gcctgtgctt ccagggggcg gtcagccgca
    69421 ttggcggggc aagctttgaa gacttccagc aggatctact gaatctgtcg aaacgtgcct
    69481 ggctggcacg taagcccatc agccagggtg gcctgctgaa atacgtccac ggcggtgaat
    69541 accacgccta caacccggac gtggtgcgca cgctgcaaca ggcggtacaa agcggcgagt
    69601 acagcgacta tcaggaatac gcgaagctgg ttaatgagcg tccggcaacc acgctgcgcg
    69661 atcttctggc aattacgccg ggtgaaaacg cggtcaacat tgccgatgtt gaaccggcaa
    69721 gcgaactgtt taaacgcttc gataccgcgg cgatgtctat cggcgcgtta agcccggaag
    69781 ctcacgaggc gctggcggaa gcgatgaaca gcatcggcgg taactctaac tccggtgaag
    69841 gtggtgaaga cccggcgcgc tacggcacca acaaagtgtc gcgcatcaag caggtggctt
    69901 ccggtcgctt tggggttact ccggcgtatc tggtcaatgc cgacgtcatt cagattaaag
    69961 tcgcccaggg cgcgaagccg ggcgaaggcg gtcagttgcc gggcgacaaa gtcactcctt
    70021 acattgccaa actgcgctat tcggtgccgg gcgtgacgct gatctccccg ccgccgcacc
    70081 acgatatcta ctctatcgaa gacttagcgc agctcatttt cgacctcaag caggttaacc
    70141 cgaaagcgat gatctccgtg aagctggttt ccgaaccggg cgttggtacc atcgccaccg
    70201 gtgtggcgaa agcttatgcc gatttaatca ccatcgcagg ctatgacggc ggcaccggcg
    70261 caagcccgct gtcgtcagtg aaatacgcag gttgcccgtg ggagctgggg cttgttgaaa
    70321 cccagcaggc gctggttgcc aatggtctgc gtcataagat ccgtttgcag gtcgatggcg
    70381 gcctgaaaac gggtgtcgat atcatcaaag cggcgattct cggcgcggaa agcttcggct
    70441 tcggcactgg cccaatggtg gcgctcggtt gtaaatatct gcgtatttgc cacctgaaca
    70501 actgcgccac gggtgtagca actcaggatg acaaactgcg taagaatcac tatcacggcc
    70561 tgccgttcaa ggtgacgaat tactttgagt ttatcgcccg tgaaacccgc gagctgatgg
    70621 cacagcttgg cgtaacacgt ctggtggatc tgattggtcg caccgacctg ctgaaagagc
    70681 tggacgggtt caccgccaaa cagcagaaac tggcgctgtc gaagctgctg gagactgccg
    70741 agccgcatcc aggtaaggca ctctactgca ccgaaaacaa tccgccgttt gataacggcc
    70801 tgctgaacgc gcagttgctg caacaggcga aaccgtttgt cgatgaacgc cagagcaaaa
    70861 ccttctggtt cgatatccgc aacaccgacc gttctgtcgg cgcgtcgctt tcaggctata
    70921 tcgcccagac gcacggcgat caggggctgg cagccgatcc tatcaaagcg tacttcaacg
    70981 gcaccgcagg ccagagtttc ggtgtgtgga acgcgggcgg cgtggaactg tacctgaccg
    71041 gggatgccaa cgactatgtc ggtaaaggca tggcgggcgg cttaatcgcc attcgtcctc
    71101 cggttggttc cgccttccgc agccatgaag caagtattat cggcaacacc tgcctgtatg
    71161 gcgcgaccgg tggtcgtctg tatgccgcag gccgcgcggg tgaacgtttc ggcgtgcgta
    71221 actccggtgc tatcaccgtg gtagaaggca ttggcgacaa cggttgtgaa tatatgacgg
    71281 gtggtatcgt ctgcattctg ggtaaaaccg gcgttaactt cggtgcgggc atgaccggcg
    71341 gcttcgctta cgttctcgat gaaagcggcg atttccgcaa acgcgttaac ccggaactgg
    71401 tggaagtatt aagcgttgac gatttggcga tccatgaaga gcatctgcgt ggtcttatca
    71461 ccgagcatgt gcagcatacc ggttcccagc gtggtgaaga gattctggcg aactggtcaa
    71521 ccttcgccac taaatttgcg ctggttaaac cgaagtccag tgatgtaaaa gcactgctgg
    71581 gtcaccgtag tcgtagcgca gcagagttgc gcgtgcaggc gcagtaaggg gtagcaacaa
    71641 tgagtcagaa tgtttatcaa tttatcgacc tgcagcgcgt tgatccgcct aagaaaccgc
    71701 tgaagatccg caaaattgag tttgttgaaa tttacgagcc gttttccgaa ggccaggcca
    71761 aagcgcaggc tgaccgctgc ctgtcgtgcg gcaacccata ctgcgagtgg aaatgcccgg
    71821 tacacaacta catcccgaac tggctgaagc tcgccaacga ggggcgtatt tttgaagcgg
    71881 cagaactgtc gcaccagacc aacactctgc cggaagtatg cggacgtgtc tgcccgcaag
    71941 accgtctgtg cgaaggttcc tgcaccctga acgatgagtt tggcgcggtg accatcggca
    72001 acattgagcg ttatatcaat gataaagcgt tcgaaatggg ctggcgtccg gatatgtccg
    72061 gcgtgaaaca gaccggtaaa aaagtggcga ttatcggcgc aggcccggcg ggtctggcgt
    72121 gtgcagatgt cctgacgcgc aacggcgtaa aagcggtcgt cttcgaccgt cacccggaaa
    72181 ttggcggctt gctgaccttc ggtattccgg ctttcaaact ggaaaaagag gtaatgacgc
    72241 gccgccgtga aatcttcacc ggcatgggta ttgaattcaa actcaatacc gaagtgggcc
    72301 gcgacgtaca gctggacgat ctgctgagtg attacgatgc cgtgttcctt ggcgtcggga
    72361 cttatcagtc aatgcgcggc gggctggaaa acgaagacgc tgatggcgtg tacgcagcgc
    72421 tgccgttcct catcgccaac accaaacagt taatgggctt tggtgaaacc aacgacgaac
    72481 cgttcgtcag catggaaggt aaacgcgtgg tagtacttgg cggtggcgat accgcgatgg
    72541 actgcgtgcg cacgtctgta cgccagggag cgaagcacgt aacctgtgcc tatcgtcgtg
    72601 atgaagagaa catgccaggt tcccgccgcg aagtgaaaaa cgcgcgggaa gaaggtgttg
    72661 agttcaaatt caacgtccag ccgctgggta ttgaagtgaa cggcaacggc aaagtcagcg
    72721 gcgtaaaaat ggtgcgtacc gaaatgggcg aaccggacgc caaaggccgt cgccgcgcgg
    72781 agatcgttgc aggttccgaa catatcgttc cggcagatgc ggtgatcatg gcgtttggtt
    72841 tccgtccaca cagcatggaa tggctggcaa aacacagcgt cgagctggat tcgcaaggcc
    72901 gcatcatcgc cccggaaggc agcgacaacg ctttccagac cagcaacccg aaaatctttg
    72961 ctggcggcga tatcgtccgt ggttccgatc ttgtcgttac cgctattgcc gaaggtcgta
    73021 aggcggcaga cggcattatg aactggctgg aagtttaagc tctgtcccag cgccgcctcc
    73081 tcagaaaaga gtcggcgctg aaattctcct ttcgttctgg aaagcgcctc gcataatctg
    73141 aagaaaacca atgtaatacg cacatctgga ttgttaccgg cagatgcccg gcgtatagct
    73201 gattccatta ttcaacactg cgccatatta agggacagat aatggaatcg ctctcagaag
    73261 gaaccacagc aggctaccag caaatccacg acggcattat tcatctggtc gatagcgccc
    73321 ggacggaaac ggtacgtagc gttaacgcgc tgatgaccgc gacatactgg gaaattggcc
    73381 gacgaattgt cgaatttgaa caaggtggcg aggccagggc tgcgtatggt gcgcagctaa
    73441 tcaagcgact atcaaaggat ttaagtctaa ggtataagcg tgggttctct gcaaaaaact
    73501 tacgccaaat gaggcttttt tacctctttt ttcaacatgt tgaaattcgc cagacagtgt
    73561 ctggcgaatt aacaccattg ggaattccgc agacaccatc tgcagaattc ccatcggcaa
    73621 agatttggca gacgctgtct gccaaatctt tccccctgcc ctggtccacc tacgtccgtt
    73681 tactttccgt taaaaacgct gacgcccgca gcttttatga aaaagagacg ctccgctgtg
    73741 gctggtctgt tcgccagcta gaacggcaaa ttgcgaccca attttatgag cgaacactgc
    73801 tgtcacatga caaatcagcc atgctgcaac aacacgctcc tgccgagacg catattcttc
    73861 cgcaacaggc gatacgcgat ccctttgtgc tcgaatttct ggaattgaaa gatgaatact
    73921 cagaatccga ttttgaggag gcgctgatca accacctgat ggatttcatg ctggaacttg
    73981 gggatgattt tgcctttgtt ggtcggcagc gaaggttacg cattgatgac aactggtttc
    74041 gtgtcgatct gctgtttttc caccgccgtt tacgctgcct gctaatcgtc gatctaaaag
    74101 tgggcaaatt cagctatagc gatgccggac agatgaatat gtatctcaac tacgccaaag
    74161 agcactggac gctaccggat gaaaatccgc ccatcggtct ggttctctgt gcagagaaag
    74221 gagtcggaga agcgcattat gcactggctg gtttgcctaa caccgttctg gcgagtgaat
    74281 ataagatgca actacctgat gaaaaacgac tcgcggatga actcgttcga acacaggcgg
    74341 tgctagaaga aggctataga ctccgttaat ttcagcggtt tcgaattctg cagacactgt
    74401 ctgcagaatc tttaaaacga actaagtttt aagccattaa atcagcctta accttcacca
    74461 caaccttttt aatctcacca ggttcgccga caacgcatcc tggtttatgc ggttctcccg
    74521 gcataaacac ggcgaacatt cccggtttta agttgatggt ttgctcgttt tcaatggcgc
    74581 tgcaaagctg gtaatcatct tcatggtgga actcttcaca ctggcgcgca gtgcctgccg
    74641 tgccaaacag aatccgttcc tcacctttta ataacagctg gatatcaatg tattgctcgt
    74701 gcaattccgc ttttttttcg acgggcgatt gagtgttaaa cgtcatgaca ttcataaaaa
    74761 tattgtcgcc ctgtaattcg taacgacccg gtgctttttc ttgcggcctg gcggctaagg
    74821 ctagtgttaa cgcgtcctgt aacgcaggat gtaacccagc agacggtaat gactgtactt
    74881 cacccatcat cataattttt ctccctgggc caacagcgca gccccaagta aacctgcatc
    74941 atggcggtag tgcgccgcca gtaaatcaac atgaaatgcc gctggctcct gcgccagata
    75001 cgtttccacc agcgccagat acccttctgc cagaccaaca ctgccaccga ccactacgca
    75061 ctggcaatcg gttgtggctt tcacatcagc aatcagcctt gccagcacat gtgcggagcg
    75121 gtgaatcagt tgctgcgcct gctcgtcgcc ctgcccggcg cgcatgaaaa tagttttcgc
    75181 atccgcgcca gccaactccc cctgcgctgc cgctgcaata ccgcgaccag aagcaatcgc
    75241 ttccacgcaa cctgtgcgtc cacagccgca gactgggccg tgtggatcgg ctagcgtatg
    75301 tccgatgtgc cccgccagac cgccagggcc ggtaagcagt ttgccgccgc tcactacacc
    75361 gccgccgacg ccagtggaaa cggtgataaa caccatctcg gttacatcgc cttccagcgc
    75421 ctgatactct gcccatgctg cggcctgcgc gtcgttaatg gcaatggtcg gcaaattggt
    75481 aagttgttcc agcgttttga ctaacggaaa gtgtaacaat ccaccaagat tatgcggatt
    75541 cagcgccagc aagctgccgt cgcgaataat cccggttgaa gcgatggcaa cccgctgcgc
    75601 atgagcttgc aatggagaga ctaatgcgga taaggcatca cgcagtgcct gcggagtctg
    75661 gctggcaggc gtaggaagtt ctcggcgatc gcggatctgc ccgtcagcgc caatcagcgc
    75721 ggcggcaagt ttagtaccac cgatatcaat cgccagcgtg gtcatagcac cgcctttttc
    75781 atcgctgcgt tgtaccactg acaaatgtgt tcaagacgcg taatggcaga accgaccgtc
    75841 accgcccacg cgccgtggcg catcgcatcc gccgcctgtg caggcgtgtt gtaacgccct
    75901 tcggcaatca cccgacatcc ggcgtcactc aacgttttca ccagcgccag atccggctct
    75961 tctggcgttt caggcgtggt atagccagaa agcgtagtgc caataatttc ggctcccagc
    76021 ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg tcatcgccag taaaccgtga
    76081 tgatgaatac gtgccagcag cgtttcaaca ggcaccggac gcgggcggtc ggtaccgtca
    76141 atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcga catcttcaat ataggccgtg
    76201 atgcgtaccg gagaatcctc cagatcgcgt ttcacaatcc cgataatcgg cacgctcacc
    76261 accgcacgcg tggcttgcag atttgccaca ccttcgatgc gaatggcaac cgcgcccgcc
    76321 tgctctgccg ctaatgccat ggcagcgaca atgtcgggtt tatcgagcgg gctgtccgga
    76381 accggctggc aggagacaat caggccaccg ttagcagcga ttttttgatc cagttgtgca
    76441 agtaacgaca tacatcttcc cttagcgaaa ggcccggtac agagaccggg caacaggatt
    76501 aacttttggt tttgactaaa tcgtttttgg cgctgccaaa cggcacggca ccgctaaatg
    76561 gtttgccgtc gatagcgtca tgagtacgca acgcttccgg gcgcagccaa cgctgaacac
    76621 gagaaggcat atccagacca atcagcagga tcaccacaaa tgtcagactg aacgagagcg
    76681 atgccagcgc agtacccaga tccagacgtt gagcgatcaa cgcgccgatg attggggcca
    76741 gtgcaccgcc caatgcgcca acgttgtagg taaagcccag gcccgctgca cgctggtcgg
    76801 tatcgaaata accgccaatc agttttggta agatcccggc gatcccttgt ccaagcattt
    76861 gctggaagaa cagtaacaga ccgagcaccc agacgtttgc gccgccaatc gcaaataccg
    76921 gaataatcag cagctgcgag gccagcaggc tacaaacgta cgctttgcgg gttcccagcc
    76981 agtcaccgag gaagccacct acgcagcatc ccacggccgc gccaaaacca ctaaagaaca
    77041 gcacattggc tacagtatgc gggttataag ccagatcggt tttcagatac gttggcagca
    77101 gcgcctgaat cggccatgag tagaggaaag caaacaacac gaccaccatc agcattacgc
    77161 cagttggcca gcgtttgccc gtactctgca ccataaagct gataaagatt gcggcgcata
    77221 acagcccaag aacagcgacg atcgcggcat tttgcaggtt accggcgaag cagaaccaca
    77281 gcgcagtagc cgccgccagt gtcattacga tattggcaat gcgatgttca ccacggtaga
    77341 gaatatccac cattgtgcgt actggtgctt tacctgcgtg tttctctttc cagtcttccg
    77401 cttccgggat gtttttacgc agccagagag caaagatgat tggcaaaatg ccgataaaga
    77461 acagcgcacg ccagccccag accggaacca ccaggctata gacctgagcg gcaacgacgg
    77521 cccctacaga gaagcctgaa atcagaaagc cactggcttt gttacgcaga tgttttggcc
    77581 agctttcaat gacataggtg gcgctggaac cgtattcacc cgccatcccc atgccgatga
    77641 ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa gccgcaggcc agcgtcccgg
    77701 ccgagaagag aacgatgctg gtgaccattg ccagacgacg cccgtagcgg tcacccatag
    77761 cgccgagcat caggccgccg aaccagcgag agataaaggc tgcggagatc agacttgccg
    77821 cctgcaccgt cgtcagcccg aattcacctt gtacttcggt gagtaccagg gcgattaaaa
    77881 cgaaatcaaa accgtcaagc agatatccca accaggcagc ggaaaatgcc cgccattgtg
    77941 cacgattgag atggcgatac cacgggatat tctgggttgt agtactcatt gtgagtctcc
    78001 cgcggtgggc gatgcccaca cgctttggta tgaaaattgt agggtacaga tgcgtttatt
    78061 tcccctcacc cggtaggggc gagcgagggg aaacaactca cccgcgctct tgcatcaact
    78121 gctgggccag cgccttcagt tctggcagat atttttcatc taccggacca aacggtttgc
    78181 ggcacagcgg cacagacacg acatccatat aatgcagaac agttttcagg ccgcggaata
    78241 cgcccgtttt gatcagtaaa tcaatgactt tattgcactc agtttgcagt ttctgcgcgg
    78301 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatat
    78361 tgtaagtact gccgataccg ccgtctgcgc ccgccagcaa gccggaagcg aagatttcgt
    78421 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
    78481 gatcgccaga ggtctgtttc agcgcgccga caccaggcag cgtaaccaag gtgttgatct
    78541 gatccagggt cagttttacc ccgctcaggg ctggaatgtt gtacaccacc atcggcaaac
    78601 catccgccga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
    78661 agtagaacgg tgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
    78721 gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
    78781 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
    78841 cttcgccggt cgaaccaccc acgtataaac cgtcaatgcc ctgctgaata ttgaactgaa
    78901 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
    78961 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
    79021 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
    79081 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
    79141 aaggcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
    79201 tcaggatcat gacgccggat cgcgtcaacg atcgcaatat gctgttgata gctaacgttg
    79261 ttatgctcgt gcagagcctg atcagccacg gttggacgtg cggcgatcag ccagtcgagc
    79321 agggcaacgt ggatcgccat gaagattggg ttacctggga tctccgccag cacgcggtgg
    79381 aaatcaacgt ctgagcgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
    79441 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
    79501 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
    79561 aggaaatctt tcgccatgcc ggaaagctca ccgatgatgg tgtccgcaga aggacgcgag
    79621 acgcgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
    79681 gcttcacgca ccgaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
    79741 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
    79801 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
    79861 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
    79921 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcagggtt
    79981 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
    80041 atgtaacgca gataaaaaca tcccgattga attatttaca agactattca cgagcattat
    80101 gaatattatg aatgtgttct tacaaaataa tcataagcgc atatttttta atgaaaaatc
    80161 acctcaccta caattaaaaa cacgacatca gcaccataaa tagacttgca aaaaatataa
    80221 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
    80281 acaatgttcg gcataattat atctgtcatc gtattaatta cgatgggcta tttgatcctg
    80341 aaaaactaca aacctcaggt ggtgctggct gccgcaggta tcttcctgat gatgtgcggt
    80401 gtctggttag ggttcggtgg tgtactcgat cccaccaaaa gcagcggcta cttgatcgtc
    80461 gatatttata atgaaatcct gcgcatgctg tccaaccgca ttgccggatt ggggctgtcg
    80521 attatggcgg tgggcggtta tgcccgctac atggagcgca taggggccag tcgcgcgatg
    80581 gtgagcttgt taagccgccc gttaaaactc attcgctcgc cgtatattat tctgtcggca
    80641 acttacgtca tcggccaaat catggcgcag tttattacca gcgcctccgg tctgggtatg
    80701 ttgctgatgg tcaccttatt tccgacgctg gtgagtctgg gagtaagtcg cctctctgcg
    80761 gtggcggtta tcgcaaccac gatgtccatt gagtggggga ttctggaaac gaactccatt
    80821 tttgcagccc aggtcgcggg aatgaaaatt gccacttact tcttccacta ccagcttccg
    80881 gtcgcctctt gcgtcattat ctcggtggcg atctcccact ttttcgtgca acgcgctttt
    80941 gacaaaaaag ataaaaatat caatcacgaa caggcagagc aaaaaactct cgataatgtc
    81001 ccgccgctct attacgccat tttacctgtg atgccgttaa tcctgatgct cggctcgcta
    81061 ttccttgccc acatcgggct gatgcagtca gaactgcatc tggtggtggt gatgttactg
    81121 agtttgacgg tgacgatgtt tgttgagttc ttccgcaagc ataaattacg cgaaacaatg
    81181 gacgatgtgc aggcgttttt tgacggcatg ggtacgcagt ttgccaacgt ggtaacgctg
    81241 gtggtcgcgg gtgaaatatt tgcgaaaggc ttaacgacga ttggcactgt cgatgcggtt
    81301 atcaggggtg cggagcattc tggtctgggc ggtattggcg tgatgattat tatggcgctg
    81361 gtcattgcca tttgtgccat tgtgatgggc tctggcaatg cgccgtttat gtcatttgcc
    81421 agtcttattc cgaatatcgc agccggacta catgtaccag cggttgtaat gattatgccg
    81481 atgcattttg ccacgacgct agcgcgcgcg gtttcgccga ttactgcggt ggtagtcgtt
    81541 acgtcaggaa ttgcaggcgt ttcgcctttt gcggtggtga agcggacagc gatccccatg
    81601 gcagtcggtt tcgtggtgaa tatgattgcc acaatcacgc tattttatta agtcattaaa
    81661 agacaaaaca ggccgccagg gcctgttttt gtattacttc acaacgcgta atgccggtcg
    81721 accaccgcgt ggtggctgcg gtggttcatc gtcaggatga gtgtcatcat cgtgatctgg
    81781 cttatcgcca tcaataaccg acataacggt ttcgttgtct gccgatgcct cttcatcatt
    81841 catgatgctg gcatcttcat cgtaggcagc ttcaggctca aacatcgtgc cagcgccatt
    81901 ttcacgggcg tagatagcca gcacggcagc cagcggcaca gaaacctgac gcggaatgcc
    81961 accaaagcgc gcgttaaagc gcacctcatc attcgccagt tccagattgc cgacagcacg
    82021 cggcgcaatg ttgagtacga tttgcccgtc acgcgcatat tccataggaa cctgcacgcc
    82081 agggagcgtc acatccacca ccaggtgcgg cgtgagctgg ttatccagca accactcata
    82141 gaatgcacgc agcagatagg gacgacgtgg tgttagctgt gacaaatcca tacagattaa
    82201 ctccggccca gacgcatttc acgttctgct tcagttaaag aagcaaggaa agagtcacgc
    82261 tcaaagacgc gggtcatata gcctttcagc tctttcgcac ccgggccgct gaactcgata
    82321 cccagttgcg gcagacgcca cagcagcgga gcaaggtagc aatcgaccag gctgaactca
    82381 tcgctcagga agtacggctt ctgaccgaag accggcgcaa tcgccagcag ttcttcgcgc
    82441 agttgcttac gtgcggcatc tgcttcagaa gcagaaccgt tgatgatggt gttcatcagc
    82501 gtgtaccagt ctttttcgat gcgatgcatg tacagacggc tttcaccgcg agctaccggg
    82561 taaacaggca tcagtggcgg atgcgggaaa cgctcatcca gatattccat aatgatgcga
    82621 gattcccaca gggtcagctc acgatccacc agggtcggaa cgctctgatt cgggttgagg
    82681 tcaatcagat cctgaggcgg attgtccttt tccacgtgtt cgatctcgaa acttacacct
    82741 ttctcagcca gcacaatgcg gacctgatgg ctatagatgt cagtaggacc ggaaaacagc
    82801 gtcattaccg aacgtttgtt ggcagcgaca gccatgaaaa cctccaggta tagtcagaat
    82861 ttttactgct accagccacc aggtggccag tcagaagttg tgttacccag taaggaacga
    82921 ctctctttgt tcgaaaatca aacaaaaaat gagcaatacc cgacatttgg gcagaaaatt
    82981 ggatgatagt ttaccagatt ttgcgacctt tgtggtgagt cgatgccgga aatggggaaa
    83041 aagagatgcg ctttagtctg aaatagttga cttagtccct tattggcgat gtggtttttg
    83101 ttttgcctgt ctgtcagacg gcagcaaaaa gcaactttcc attttttacg ctgattcaga
    83161 ttttagccat aaaaaaaccc gccgaagcgg gttttttcgc aaattgtttt ctgccggagc
    83221 agaagccaat taacgtttgg agaactgcgg acgacgacgt gctttacgca gaccgacttt
    83281 cttacgttca acctgacgag cgtcacgagt aacgaagcca gctttacgca gttcagaacg
    83341 cagggactcg tcgtattcca tcagagcgcg ggtgataccg tgacggatcg caccagcctg
    83401 accagagata ccaccacctt taacggtgat gtacaggtcc agtttctcaa ccatgtcgac
    83461 cagttccagc ggctgacgaa ctaccatgcg agcagtttca cgaccgaagt actgttccag
    83521 agaacgttgg ttgattacga ttttaccgtt gcccggtttg atgaaaacgc gagctgcgga
    83581 acttttgcgg cgaccagtgc cgtagtattg attttcagcc attgcctata atcccgatta
    83641 gatgtcaaga acttgcggtt gctgtgccgc gtggttgtgc tcgttacccg cgtaaacttt
    83701 cagtttacgg aacatagcac gacccagcgg gccttttggc aacatgcctt taaccgcgat
    83761 ttcaatcaca cgctcaggac ggcgagcaat catctcttca aaggtcgctt gtttgatacc
    83821 accgatgtgg ccggtgtggt gatagtacac tttgtcagta cgcttgttgc cggttacagc
    83881 aactttgtca gcgttcagaa cgatgatgta atcaccggta tctacgtgcg gagtgtattc
    83941 cgctttgtgc ttaccgcgca ggcgacgagc cagttcagta gccagacggc ccagagtttt
    84001 accggtcgcg tcaacaacat accagtcgcg ttttacggtt tctggtttag ctgtaaaagt
    84061 tttcattaaa agcttaccca ataaatagtt acacgttggt gaacacccaa acgtcttcaa
    84121 ttgttgaggt tcacacgaca aagtccggca aacctacccc ttcgaatagc ctatgccagc
    84181 acacaaaaag ttttgggaaa aaaactttct tgtaacgtgg ggtcgcagga ttatagagaa
    84241 gtcggggtca aagatcgacc cctttttgtg atttgtgaca ggttttaacc cgccaaatgc
    84301 tcgcgcttca gatactcttc gctttgcatc tcttgcagac gtgacaggca acgctggaac
    84361 tcaaatttaa gtcgctcgcc ctgataaatt tcatacagcg gcacttctgc actcaccact
    84421 aatttgacat ggcgctcgta aaactcatcc accagcgcaa taaagcgccg cgcttcgctc
    84481 tccatcaacc gcgtcataac tggtacatca aacaacatga ccgtatgaaa gagacgtgag
    84541 agcgcaatat agtcatgctg actgcgggcg tcgacgcaca gcgtagtaaa agagaccgcc
    84601 agcgtctggt tctcgacgcc cattgtcgct aatggccgat ggttgatttc taacgttggt
    84661 gaaatttctc gtttcgcccc cgccagcgcc aaccatagtt tatccatttg cgcccggatt
    84721 tcatcgttga gtggcgaaag ccacagatgc gcctgagtga gtgtacgcag tcgataatca
    84781 acaccagcgt ccacgttcat tacatcacaa tgctgtttaa tggcatcgat tgcaggcaga
    84841 aaacgcgcac gttgcaggcc atttcgataa agttcatccg gcggaatatt tgacgtcgct
    84901 accagggtaa taccgcgggc gaacagggct ttcatcagac cgccaagtag catggcgtcg
    84961 gtaatatcag aaacaaaaaa ttcgtcaaaa cagagcacgt cagtttcggc tttaaagcga
    85021 tcggcaataa tttccagcgg atcggtctgc ccctgtaagg cagttagctc ttcgtgcacc
    85081 cgcagcataa aacggtgaaa gtgcaggcgc tgtttccgct ctcccggcag gctttgatag
    85141 aaaaggtcca tcagccaggt tttcccgcgt cctacaccgc cccacatata taagccacgc
    85201 actggcgtat gctttgtgtc ttcgcgttta ccccacagct taccgacccg cgccattagc
    85261 ccactcgtcc tgggggctgg tggcctgcta ttgatgagtt cctgataaat aatttccagg
    85321 cggctgacgg cctctttttg aacgtcgtcg ggttgatggc tgccttcatt gagcgccttc
    85381 aggtattgcg atgttggggt aacgctttgc atgatcttat tgttattcct tgaataatcg
    85441 gtgcgccgtt gttcacggtt gacgaaaaaa aggccgttct acactacgcg atatgcagtc
    85501 gggattccac ttctgtggaa ttaacggtta tagtggcata atcagccgca ggcatggagc
    85561 ctgaagccaa caccctacgg aaacaaaaga caacgggaga tgttcatgac ctgggaatat
    85621 gcgctaattg ggttagtcgt cggcatcatt attggtgctg tggccatgcg ttttggtaat
    85681 cgtaaactac gccagcaaca ggcgttgcag tacgaactgg aaaagaataa agctgaactg
    85741 gacgagtatc gcgaagagct ggttagccac tttgcccgca gcgcggaatt actggatacc
    85801 atggcgcacg actatcgcca gctgtatcag cacatggcga aaagctccag cagcctgctg
    85861 ccggaactgt ctgctgaagc aaacccgttc cgtaatcgtc tggcagagtc tgaagccagc
    85921 aacgatcagg caccggtgca gatgcctcgc gactattctg aaggtgcatc cggcctgctg
    85981 cgtactggcg cgaagcgcga ctaatttatt tttcgggcgc agccattgcg ccctcctctt
    86041 ctctccctcc ccgactatca tttaatctgg tgtctcattg ttagccgtct gaaaattcaa
    86101 taacatcgaa ctgttttgaa tctcttttct tatcattcag gtacgagagc aggaataatg
    86161 aaaaaacaaa cccagctgtt gagtgcatta gcgttaagtg tcgggttaac tctctcggcg
    86221 tcatttcagg ccgtcgcgtc gattccaggc caggttgccg atcaggcccc ccttcccagt
    86281 ctggctccaa tgctggaaaa agtgcttccg gcagtggtga gcgtacgggt ggaaggaacg
    86341 gccagtcagg gacagaaaat cccggaagaa ttcaaaaagt tttttggtga tgatttaccg
    86401 gatcaacctg cacaaccctt cgaaggttta ggctccggtg tcatcatcaa cgccagtaaa
    86461 ggctatgtgc tgaccaacaa ccatgtgatt aatcaggcac agaaaatcag tattcagctc
    86521 aatgatgggc gcgagtttga tgcaaaactg attggtagcg atgaccagag cgatatcgcc
    86581 ctgttgcaaa ttcaaaaccc gagcaaatta acgcaaatcg ctattgccga ctccgataaa
    86641 ttgcgcgtcg gtgattttgc cgtggcggtc ggtaacccat ttggccttgg gcaaaccgcc
    86701 acctctggca ttgtttccgc attaggacgc agcgggttga atcttgaagg tctggaaaac
    86761 tttatccaga cagatgcttc cattaatcgc ggtaactccg gcggtgcact gttaaacctt
    86821 aacggtgagt taattggcat caacactgca atccttgcgc ctggcggcgg gagcgtcgga
    86881 attggatttg ccatccccag taatatggcg cgaacactgg cgcagcagct tatcgacttt
    86941 ggtgaaatca aacgcggtct gttaggcatc aaaggcaccg agatgagtgc cgatatcgcc
    87001 aaagccttca accttgacgt gcagcgtggc gcgtttgtca gcgaagtgtt gccaggttct
    87061 ggctcggcga aagcgggcgt caaagcgggc gatattatta ccagcctcaa cggcaaaccg
    87121 ctgaatagct ttgctgagtt gcgctctcgt atcgcgacca cagagccggg cacgaaagtg
    87181 aagcttggcc tgctgcgtaa cggcaaacca ctggaagtag aagtgacgct tgataccagt
    87241 acctcttcgt cggccagcgc tgaaatgatc acgccagcgc tggaaggtgc aacgttgagc
    87301 gatggtcagc taaaagatgg cggcaaaggt attaagatcg atgaagttgt caaaggaagc
    87361 ccagctgctc aggctggctt gcaaaaagac gatgtgatca ttggcgtcaa ccgcgatcgg
    87421 gtgaactcga ttgctgaaat gcgtaaagtg ctggcggcaa aaccggccat catcgccctg
    87481 caaattgtac gcggcaatga aagcatctat ctgctgatgc gttaatgtcg taaaccgggc
    87541 atcaggctga cgtgtgatgt ccggttaact cgtggtatgc tgctgccgtt ccctttttta
    87601 atgacgcctc catcatgttt gtgaagctct tacgttccgt tgcgattgga ttaattgtcg
    87661 gcgctattct gctggttgcc atgccttcgc tgcgcagcct taacccgctt tccactccgc
    87721 aatttgacag taccgatgag acgcctgcca gctataatct ggcggttcgc cgcgccgcgc
    87781 cagcggtggt taacgtttac aaccgtggtt tgaacaccaa ctctcacaac cagcttgaga
    87841 tccgcaccct gggatccggt gtaatcatgg atcaacgcgg ttatatcatc accaataaac
    87901 acgtcatcaa cgacgccgat cagatcatcg tcgccttaca ggatggacgt gtatttgaag
    87961 cattgctggt gggatctgac tctctaaccg atctggcggt acttaaaatt aatgccactg
    88021 gcggtttacc taccattcca attaatgcac gtcgcgtacc gcacattggc gacgtagtac
    88081 tggcgatcgg taacccgtac aacctcgggc agaccattac ccaggggatt attagtgcca
    88141 cgggtcgaat cggtctgaac ccgaccgggc ggcaaaactt cctacaaacc gatgcttcca
    88201 ttaaccacgg taactctggc ggcgcgctgg tgaactcgct gggtgaactg atgggcatta
    88261 acacgctgtc gtttgataag agtaacgatg gcgaaacgcc ggaaggtatc ggctttgcga
    88321 ttcctttcca gttagcaacc aaaattatgg ataagctgat ccgcgatggt cgcgtgatcc
    88381 gcggctacat tggtatcggc ggacgcgaga tcgcaccact gcacgcgcag ggcggtggta
    88441 tagatcaact gcaagggatc gtggttaatg aagtgtcacc tgacggcccg gcggcgaatg
    88501 cgggtattca ggtcaacgat ctgattattt cggtggataa caaaccggcc atctctgctc
    88561 tggagacgat ggatcaggtg gcagaaattc gccctggttc ggtgatcccg gttgtagtga
    88621 tgcgtgatga taagcagtta acgctgcagg tcaccattca ggaatatccg gcaaccaatt
    88681 aagtcgagtg ctcaaaacaa aaaaccggag tctgtgctcc ggttttttat tattcgctaa
    88741 tcaattactt attaacgaac tcttcgccca gggcgatatc tttcttgagc gtatccagca
    88801 taccttccag cgcgttctgt tcaaatgcgc tcagggtacc gatagattta cgctcttcca
    88861 cgccgttttt acccagcagc agcggttgag agaagaaacg ggcgtactga ccgtcgcctt
    88921 caacgtaggc acattcgaca acgccttgtt cgccctgcag tgcacgaacc agagacagac
    88981 caaaacgtgc agctgcctgg cccatagaca gggttgcaga cccgccaccg gctttcgctt
    89041 caaccacctc agtacccgcg ttctggatac gtttggtcag atcagccact tcctgctcgg
    89101 taaaactaac gccaggaacc tgtgacagca gcggcagaat ggtaacacca gagtgaccgc
    89161 caataaccgg cacttcaact tcgcctggct gtttgccttt cagttccgca acaaaggtgt
    89221 tggaacgaat gatatccagc gtggtaacgc cgaacagttt gtttttgtca taaacaccgg
    89281 cttttttcag cacttcagca gcaattgcaa cagtggtgtt aaccgggtta gtgataatac
    89341 caatgcacgc tttcgggcag gttttcgcaa cttgctgtac caggtttttc acgatgccgg
    89401 cgttaacgtt aaacaggtcg gaacgatcca tacccggttt acgcgctaca cctgcagaga
    89461 taagaacgac atctgcgcct tccagcgccg gagtcgcatc ttcaccagaa aaacctttga
    89521 ttttcacagc agtagggata tggctcagat cgacagccac accgggagtc actggagcga
    89581 tatcatacag agagagttct gaacctgaag gcagttgggt ttttaacagt agtgcaagcg
    89641 cctggccaat accgccagca gcgccgagga ctgcgacttt catcctaaac tccttattat
    89701 attgataaac taagatatgt tgctccgctg ccgcgacctt aatccacaaa attgccgttt
    89761 acaatgacca catctcaaga atgtgtagtc acgcaagttt agcgtttatg catttaattg
    89821 ccgtaatcag gaacctaact tacgtaatta acagtcgctt atgcattagc gcaacattcc
    89881 aacaggtggt gacaatatac cctaccgttc agccaaaaca acatcaattt gataacaatt
    89941 aatttacttt taagcagaat ttgcatgccg tgacgcaggc atgtttctca ataacgaaat
    90001 ttgataaaat cccgctcttt cataacatta tttcagcctt cttcagggct gactgtttgc
    90061 ataaaaattc atctgtatgc acaataatgt tgtatcaacc accatatcgg gtgacttatg
    90121 cgaagctcgg ctaagcaaga agaactagtt aaagcattta aagcattact taaagaagag
    90181 aaatttagct cccagggcga aatcgtcgcc gcgttgcagg agcaaggctt tgacaatatt
    90241 aatcagtcta aagtctcgcg gatgttgacc aagtttggtg ctgtacgtac acgcaatgcc
    90301 aaaatggaaa tggtttactg cctgccagct gaactgggtg taccaaccac ctccagtcca
    90361 ttgaaaaatc tggtgctgga tatcgactac aacgatgccg ttgtcgtgat tcataccagc
    90421 cctggcgcgg cgcagttaat tgctcgccta ctggactcac tgggcaaagc agaaggtatt
    90481 ctgggcacca tcgctggcga tgacaccatc tttaccaccc ctgctaacgg tttcaccgtc
    90541 aaagacctgt atgaagcgat tttagagctg ttcgaccagg agctttaatc tctgccccgt
    90601 cgcttctggc ggcggggaaa atgttgctta tccctctcaa ccccctgctt tcccctgcga
    90661 ttaatttaac gaatagtgcg ttttaccgcg acatatcatt cacatagtga atacatacgg
    90721 taaaaaaagc acattatgca aaattcatta tctgattgaa aaaactggca tttataataa
    90781 ataaccgtgt ttttaatact tttttgttat taaaattcac atttttaaca cttagtatca
    90841 actgaaacag ttagcgcggt attaattagc tcaataatta gtgtatactt gattttgtga
    90901 tatgggtcac gaaacaaagg cccagctaaa agattatgtc gaggtaaaaa tcatgaaaat
    90961 caaaaccact gttgctgcat taagcgtact ttctgttctc tctttcggtg cattcgctgc
    91021 cgactccatt gatgctgcac aagcacaaaa tcgtgaagca atcgggaccg tatccgtaag
    91081 tggtgtggcg tcttcgccaa tggatatgcg tgaaatgctg aacaaaaaag cggaagagaa
    91141 aggcgcaacg gcctaccaga ttactgaagc tcgtagcggt gacacctggc acgctacggc
    91201 tgaactgtac aaataaaccc tcatcgtctt gtccgacgat attgcccccg gttcgggggc
    91261 ttttttttgc gctaatgacg aacattaaaa cgcaaatgcc cttccagctc ttcctctgcc
    91321 tcatcaaaca gcaatattaa cgcgccaaaa cggcgacgcg ttttctctcc cagatgaaca
    91381 aattcaatct caagtggtag cggcaggaca tcgttcatta acacatccca tagtgagtcg
    91441 agatcgcgta ccttatcttt cgccagacca aaggtttggc taaagtcacg ataaaaatcc
    91501 tcctgactct caatctcatc aaaatcaaag gtataaatat tcatctgttg ccaccgtcac
    91561 gtttcgcggc gagttctgcc gtgcatcgct aagtatagcc atgaaaaaac cgacgctttt
    91621 ggcgtcggtt ttgacttaac tatcagtcaa cgcatgttga tacttatgga gcatccccgc
    91681 cagccgatgt accggttccg tcacctgcgg tggcgcttgc cagatgcgta gcttttcctg
    91741 gtagatttca agttcttcca gcaactggcc aaagtaccgg cgacgtttat catcgctacg
    91801 ggcagatatc acatggtctg ctgtgcggcg catttgtcgg tgaaacgccg ataaatcctc
    91861 gttaaccggg atcggcgcat cacgcaggcg ctggtgcgcg ataatcatcg tcagcgccag
    91921 gcgaaatttc ggcaaatccc ctgggaactt attcatcagc aaaaacagct gctgataaag
    91981 tgccgggagg tggttctctt tacgacgtgc cacattggta gtcatcgcgg aaacagcggc
    92041 agaaacaaac tgattaagca gtacacgtcc agtcctgtcg cgcgatttat cccgcaccag
    92101 caaaataacg gtgaacgcga gcacacagcc gacgatttgc cctaatgcgc tgtcgagaaa
    92161 ctgactgaaa tggaaagtca tcgggttatc cagcacgata atatttatgg tgctggccag
    92221 cgcccccatc gagcccagtc gccgtttctg tacttctatt ccgaggaaga atcccagcac
    92281 tgccaggcta atacacagca gcaacatgct ctgttgagta ttagggataa tcaccaaaaa
    92341 gtagagcagc cctaacggca gcgcggccag cgtcccgtag ataaagtcga tcgccaccat
    92401 gcgtggattc ggcaaacgca ttgccagtga cgtcactacc gcaatcatca ccattgcacc
    92461 actgccggaa gtccagcccg tccacagcca gaaaagcgtg cccagaatgc aggaaagtgt
    92521 ggttcgccag aagttaacca ttgcatgatg acgttcggct gactctactt tgacttccgg
    92581 ttcgccttgc aggatctctt cttcggtggc gttgattttt gtgttactga taacgccgcg
    92641 cttgagaagc tgataacgcg ttgccgccgc gacccagcta taaatggtga caggcgtttc
    92701 ccgttccccg gtccaggcga taactcttcg caggcgtttg agctgcttgt ggacgtcctg
    92761 cgcggtttct accggcgtgt caaaaaattc gcggaaagta tcagtgatca attccgggcg
    92821 cgtattttga ataagataag tttcgcagga ttgggtaatc agcgtcagcg atagcgtatt
    92881 gatcgctttt aaacgtcgat tggcccgcgc ccagcgggaa gattccatat tcaggttgct
    92941 gcgcatgcct tgtagcgccg tggtgcgtcg aaccaggtcg ccccaggctt tatcgacaac
    93001 ttcaccatcg ccatgcttga tacagagttg cattaattga tattgcgcga ccagcaaact
    93061 ttccagctct cgatccactt cttgtttgat cgatcgcgga gaaaagagca aatccgccat
    93121 aatcgcacac acaataccga taacgatctc gctacaacgt tcgacggcaa actgcggcgt
    93181 aagcaatggt tccggctgaa tggtgatcac aatgatcagc gcggtataac cggccagccc
    93241 ccacgcatac gagttttcta ttcgtaccag cgaggatatc caggtacaaa aaccggccca
    93301 gatacagcac accagaatca tcaataatgg tgcgcggatc atcgcaatga tgatcaccag
    93361 tccggcaata cagccaataa atgtgccgat gatgcgcaaa aagccacgat agcgaatagc
    93421 gccagaatac ggttcacctc ccgcagcaaa ggccggaccg gcggcaacaa tcgccgctgt
    93481 cagtaccgcc cagcgtggcg tttccagctg gaagtgaaag ccaacaaaca gcgccagtac
    93541 aatggcggtc gccagtttta ccgcaaagcg aatatgttgg ttagcaatgg agaaaatacc
    93601 catcgtgatt aaccaaactc acgcaggcga tgggccattt tacggaagaa cgaatcctgg
    93661 ctttcgtcgc gatcttgttt gccagtgacc accactgtag cagtggtgcc cgcaggccag
    93721 atgttctctt gctggttgtc gagacgaata cgaaccggaa cacgttgcgc caaacgcacc
    93781 cattcaaggt tagagtcgat ggtcgccatc cctttgtcgt cacgcgtgct gctggcgttg
    93841 gtgaccccag cggcaacact atcaacagtc cctttcagca ctttgttact gccaagcggc
    93901 gtgatctctg cacgataccc agggcgcacc ccttccagct tagtttcttc catataggcc
    93961 agtacataga aagagttctg tttcaccagc gcaaccgccg ttgatccacg agtaataaac
    94021 tcaccggtat agacgttgag gttggtcacc cagccatccg ctggcgcgcg gatcaccgtg
    94081 cgttcaagat ccagttttgc cagatcgcgg gtcgcctgcg ctttcgctaa ctgatgcaga
    94141 accgtttgta gtacgttgtt ggcctggtcg atctcttcgc gagacatcgc ctgcacaccg
    94201 agacggttac gacgtccggc ctcctggcgt ttctcctgtg ccagtacctg ataataagca
    94261 acatcggctt gcgcttcctc aagcgccttt tgatagcgcg gctggtcgat ggtgaacagt
    94321 acctgtcctt ttttcaccag ctggttatca tgaacattca cctgggtaat gagtccagaa
    94381 acgtccggcg cgatcgcaac gacgtcagcg ctaaagcgcg cgtcacgcgt ccagggggat
    94441 tcggtgtaat agacccaggc attaaaaatt gcgatgaagg ccagaatgac taatacgacc
    94501 gtgatggccg tacgggagaa ttttcttatt agtgttttca cttcaacctc aaacgaacag
    94561 tcgcgatatc aaataaaaca agcagcaata gagcgcggtg ttgaacaacg ccggatgcca
    94621 gacaaagtcg tagatacctg ttggcacaag tacccggcgc accagccaga aaatcgccag
    94681 tgataaaagc aattcaaaaa atatcggtgg gaaggacagc ccaaacacca cgataacggg
    94741 aaacagactc atgttgacct tggttgtata gagagagcag gcgttattat tttcagcatc
    94801 tgtcgccgca gagaagggca tggaaagccg ggcgagagca acattgctgt agattgatat
    94861 ttaatatatt agcgtaactg ttatgctgtt atctatatta tgtgatctaa atcactttta
    94921 agtcagagtg aataatggaa cgactaaaac gcatgtcggt gtttgccaaa gtagttgaat
    94981 ttggctcttt taccgccgcc gccagacaac tacagatgag cgtttcgtcc atcagtcaga
    95041 cggtgtcaaa actggaagat gagttgcagg taaagctgtt aaaccgtagc acacgcagca
    95101 ttggcctgac cgaagccggt agaatttact accagggctg ccgtcgtatg cttcatgaag
    95161 tgcaggatgt tcatgaacaa ctgtatgcct tcaataacac ccccatcggg acgctacgca
    95221 ttggctgttc ttcaactatg gcacaaaatg ttctcgccgg gctgacagcc aaaatgctga
    95281 aagaataccc aggtttgagc gtcaatctgg ttaccggaat tccagccccc gacctgattg
    95341 ccgacggtct ggatgtggtg atccgcgtcg gcgcgttgca ggattccagc ctgttttccc
    95401 gccgtcttgg cgcgatgcca atggtggtgt gcgccgcgaa aagctatctc acacaatacg
    95461 gcataccgga aaaacccgcc gatttaagta gccattcttg gcttgaatac agcgtgcggc
    95521 ccgacaatga atttgaactg attgcaccgg aagggatctc gactcgcctg atcccgcaag
    95581 ggagatttgt gactaatgat ccgatgacgc tggtgcgctg gctaacggcg ggtgccggga
    95641 tcgcctacgt gccgctgatg tgggtgatca acgagatcaa tcgtggggag ctggagatcc
    95701 tgctgccgcg ttatcagtca gatccacgcc cggtttatgc gttatatacc gaaaaagata
    95761 agctgccgct gaaggtacag gtcgtgatca actcgctgac ggattatttt gttgaggtcg
    95821 gtaaattgtt tcaggagatg cacgggcgcg ggaaagagaa gtaattcata ttgtactgtt
    95881 acgttgttca aacctgtgcc aacgggttcc cctcacccta accccagagg agcgagggga
    95941 ccgttcgtgc acgtagaaag attaattatc cttctgaaaa tactgaaatt attacgcagt
    96001 accgccaaca gtcaggttat caactttcag cgttggctgg cccacgccaa ccggcaaact
    96061 ttgcccttct ttaccgcaga cacccacgcc gttatccagt ttcaggtcgt tgccaaccat
    96121 cgaaatctgc tgcatggttt cgataccgga accaatcaac gttgcgcctt tcaccggctt
    96181 cgttacttta ccgttttcaa tcagatatgc ttctgaagtg gagaaaacga atttgccgga
    96241 ggtgatatcc acctgaccgc caccaaagtt cggtgcatag ataccgtact caacggattc
    96301 aataatttct tgcggggtcg atttacccgg cagcatatag gtgttggtca tacgcggcat
    96361 gggcagatgg gcgtaggatt cacggcgacc gttgccagtc ggcgtcatcc ccatcaaacg
    96421 cgcgttgagt ttatcctgca tgtagccttt cagaatgccg ttctcaatca gcacgttgta
    96481 ctggcctggc gtaccttcgt catcaatcgc caccgaacct cggcgatcga ccatcgtgcc
    96541 atcatcaacc acggtacaca gttctgaagc caccagctcc ccgacctgtc cactaaatac
    96601 tgaagtgcca cggcggttga agtcgccttc cagaccgtga ccaaccgctt catgcaacag
    96661 cacgcccggc caacctgcgc caagtactac cggcatggtg cccgctggtg cagcaacggc
    96721 agaaagattg accagcgcca tacgcactgc ttcttttgcc catgcatccg cacggacttc
    96781 gccgtcgaga tcggcaagaa agaactcata accaaaacga ccgccgccgc cactggcacc
    96841 gcgttcgcgt ttgccatctt cttcgaccag aacgctcacg gaaagacgca ccagcggacg
    96901 gacatccgcc gctagcgtgc cgtcggtggc cgcaaccaga attaattcat agacaccact
    96961 gaggctggca gtcacttcct gtacgcgctt gtccgcttcg cgagcaacct tatcgacgcg
    97021 acgcaggata tccagcttct cttcacggct catgctttgc agcggatcta ccgaggtata
    97081 caacgggcta tgctctaccg cgcccagcgt ctgtactttg ccatcaccac tatcacggac
    97141 gatggtgcgc gccgcttgcg cactctgttc cagcgccagc aggctgattt ggtcagcgta
    97201 agcaaatccg gttttttcac cgctgattgc acgcacgcca acgccctgat cgatgttgta
    97261 agaaccatct ttaataatac ggtcttctaa aacccaggat tcgtgatagc tcgactgaaa
    97321 atagagatcg ccataatcaa ggcgacgttc ggccagttga ccgaggatcg cgaacaagtc
    97381 ctgatgtttc aggccgttcg ccgctagcaa ttgttcactt accaggttca gactcatcgt
    97441 ttttgctact cgttagttac tgcagtagag gattttttac ggctgccgga tgcggcgtaa
    97501 acgccttatc cggcctacga ttatgttccg gtttgtaggc ctgataagac gcacagcgtc
    97561 gcatcaggca acggctgtcg gatgcggcgt aaacgcctta tccgacctac ggttatgttc
    97621 cgtttgtagg cctgataaga cgcacagcgt cgcatcaggc aacggctgcc ggatgtggcg
    97681 tgaacgccct atccgaccta cggttatgtt ctggctcgtt ggcctttggc aacgattatc
    97741 ctatgagatt ggggcaatta cgcgccctcg tcaaatcatt gcgctttttc tttacgcggt
    97801 tggcgcaaca cttcgttgat ttgcggatcg tccagcggac ccgaaatgtg atagcgcaaa
    97861 atggagactt tgctccacag cggccccagc actttactgg cggcaaacac tgccgcgcca
    97921 acaatggggt taaccgcaaa agccgcagcc acgcccaccg tcgcagaaat ctctggtgcg
    97981 acaaccgctt ccatattcag gtcgcgacgt accagattta ccgacccttt catggcgata
    98041 tccgcctcca ggccatccac caacgtgtcg tcggtgtgca taacgccgtc tttaatccac
    98101 gcggtgctgc gaatggagtc aaaatagaac ccttcgccaa aagtgtctct gaaatcaaaa
    98161 cgcagcttac gcatcagggc atctacgctc aataagcgca gcaattgccc ggcatgtccg
    98221 gtattgattt cggtaatttc gcctttaccc agttgagtat gaatgatgcc attcaacgtc
    98281 gcctcatctg gctgccacgg tgctttacgc cagtgtaaat cgtaatccac attaaatgac
    98341 gactgccgta tgggcgtcgt gacaccaaaa aattctgcgg cggcatcaat tttctgcccg
    98401 cgcagttttc ctttcagcga ggtacgttca ttccccggat tattaaccca ttcaccatcg
    98461 gcagtaagcc gcgagaaacc ggtatcaatc agtccattgg tcagtgttaa tgtatcgcca
    98521 gaaatggtga gatcactgtc aatacgaccg aatttttgcc cccagaacca gcactctgcg
    98581 catcgtattt gtgcgtccgg ccagccacgg aagttaatgc gctccgttgt cgggaaaggt
    98641 gatgacggcg ttgaatcacc acgagttttc gccacgctcg ggttgtaata aagatatttg
    98701 atattcgcca gccacggcgc gttattacgc atcgccagcg tggcgttgat ttcacgccct
    98761 tgcgcctcaa cctgggtgcc atttgccgtc ggttgcgaaa caatactcag gttattccat
    98821 tgctgatttc ccagcgacaa cataggcgta cgtaacgtta tgtgttgtgg gaaactcgct
    98881 gcaccaccga cactctccgc cgcacctttc tgaaacaggg ccagccactc ggcaccattc
    98941 atcggcggca tattgagttc aacgccactt tgttccggca acggcgggag cgttttactg
    99001 tctgccgccc aaatagcacg gtcgagcgtg agcttttgac cgagcaacca gcggctattg
    99061 aaatgattat ccgcaccagc ctgtccggtt aattcaaagc tgttgagatt gccatcaacc
    99121 ttaacgttta ccgctagtgg ttcacccgca gatttggcta acggtgaagg taagtgactg
    99181 ctcacattct tcagatcgcc gttcagctct acgttatagg tcgcaccagc atgataaggc
    99241 agatcaatgc ccactttacc atcccacgcc acgctgccac tcaatgcttc gttcaccgct
    99301 tcaggcagaa cgccggtttt cgctggttgc cagttaccgt tgagattcac cgcgacctgg
    99361 taggcttttg ccccttcttt ggtggaaaaa tccacgttca acggctgatt aaaccagctt
    99421 gctgtcagtg gttcactttg cagatcgcca ttgataaagc tgaatttacc gctcaaattt
    99481 ttcagggtgc tgtcgagtgg tttgataaac agactgttat tacgcagcgt cacatcacct
    99541 ttcgcggtta ccagttcgcc gttcagcggg atatcaagat gtaagcgagc attcacatcg
    99601 ccgtcgagct ggagttcttg cagggtcgcc cccaaagaat ctttcagcgg tgtctcatca
    99661 aagtaagggc caacggcttt acccggacct ttaatgtcag cgtcaatcag cagtttttct
    99721 tttgagtagt cagggatcac tgcggtgaga ttactcgcgc gcacgccacc cagattaacg
    99781 ccatcggttt tcatccataa accgtcgtta ataaagtcca gttcaatatc aaggttagtt
    99841 aatgcaggcc agtccggctg gaaggcaaac ttcgcgttgc gcagcggcac cagcacttca
    99901 aactgacctt cgttgtgttt ataggggaag agttgcggat tgccaccata aaccagcgtc
    99961 gcgttatccg cttcaccgcc ctgaatcgcg ccacttaagt aatcaaccag gtctttaccc
   100021 atcaagtttt ccgggaaata gcgccaggct tgtgaaccat catcggtact gatgccagcc
   100081 agaataccca gccagggttc atcgttagca ggttgcaggt aacgaaaacc gccgcgcgca
   100141 tggacggctt tggctttaac gtcaatatta cgcccatcca gctggaaacc tttgtcattg
   100201 ttcagccagc ttatagttgc ctggccgtcg gcgatttcca gtggcgcacg gaataccgtt
   100261 tcgtaaggca tctttgcctg cttcatcgac gcggtaagca aaccattttc aacgctgccg
   100321 gaaagcgtcc cggagaagtg ttccgcaccc ggtaataatt tccattgctt ccaggccaga
   100381 tcgctccacg atgcctgaaa acgggtcttg tctgccgcct gaagcgggat atccagcgcc
   100441 agagtgttaa tcttgccgct cggttgtgtg gagcgccaaa catcacccag tgcaggtgaa
   100501 agtttcgcgg ccagcgggcg tatgccctcc aggcctgcca gctccagatt actggcgcga
   100561 atccggagtt cgtcactgcg tttattgtct ttgccgccaa cgtcctgttc cggtatccag
   100621 gccagcgtca atgctccgct cggccagggt ttgccgtcca tcgtgatccg tgtatcggga
   100681 atagagaact gccagcccgg attttcacgc gtaatatgcg cggtcagatt atccaccgac
   100741 agcgtatgcg tttccttctc gcccaaccag ctggcaccgc cctgtttcag ccagacgtcg
   100801 ccaccggtta catcgccttt gtcgatcgtc atccagcctt caagggagaa ctgcgccgtt
   100861 tccagggcaa tattgtcctg catccattta ccgagccacg gcttcaggtc gatgtcatcc
   100921 gcctggagcc agacgcgacc attgcttaac aacccctcat catcgcgcaa atccatgcgc
   100981 acctgcatca cgccgtgctg tccggtaagg ctggagaggc ttaccaggcc ttccgcacgg
   101041 tgtcgacgtg gatcgttcag ccaggtgagt tgtgggatcg ccagctcggc gcgctgaccg
   101101 gatggcgtca ggaaactgac ttcactgtcg cggagatcga aatggtcaaa ttgacgaaga
   101161 aacagatcgc tgatgtgact ggcttccaga ctgtcatcgc taccaccgct ggtgatagga
   101221 gtgttggtgc gaaagcgcag ctgccagaaa gtgaggtcgc gaaactgcca gcgcatatgt
   101281 aacaggctct gccagacatc cagcgccaga gtaacgcgtt taaccgaaaa ttcgccgcca
   101341 tcttttagtt ctgcacggat gtcgtgtgct tcaagcgtcg ggccaaaatt ctgccagctg
   101401 gctgagagct gactggcttc aaccggcatg ccagtcgcgg attctatttt gttgaggatt
   101461 tccggacgcc aggcgtcaag atgcggtaaa gcaatacgca ggccgctaac cagcagggca
   101521 gcgatcacaa cgagcgcggc tccagtaagc agtaaaatcc ccggcaatcg cctcacccgt
   101581 cactccttgt ctgctaaata tgtgactcaa aaaccctttg ccggatggcg gcccagcatc
   101641 tgtttacatc attacgacgt caaactgctc ctggttatag agcggttcaa tttgtacttt
   101701 aacctgttta ccaacgaaaa tttccacttc cgccagcgag tgtgactctt cgcctttcaa
   101761 ggcttcagct actgccggag aagcatagac caggaaacgg tcggagtcgt aagcatggtg
   101821 gacacgaaca atctcgcgca tgatttcata gcataccgtt tccacggttt tcaccgttcc
   101881 gcgaccgtgg caggttgggc attcgttaca cagtacgtgc tcaatgcttt cgcgggtgcg
   101941 tttacgcgtc atctccacca gccccagcgc cgaaaaacca ttaacgctgg ttttcacccg
   102001 gtctttgctc aatgcctgct ccagcgagtg cagcacgcgt cggcggtgat cttcattatt
   102061 catatcgatg aaatcaataa tgataatccc gcccagatta cgcaaccgta actggcgagc
   102121 gatagcctgc gtcgcttcaa tattggtatt gaaaatggtg tcgtccagat tgcgatgacc
   102181 gacaaacgct ccggtattga tgtccacggt ggtcatcgct tcggtctggt cgataatcag
   102241 ataaccaccg gatttcagtt ctactttgcg ttccagcgct cgctggattt cgttttcgac
   102301 atcaaagaga tcgaaaatcg gctggcgtcc ggtgtaatgc tccagcttgc tggtcatctc
   102361 gggaatgtac tccgaggtga actcaagtaa tgcttcgtaa gtcaggcgtg agtcaacgcg
   102421 aatgcggtcc agttcggcgt cggcaaaatc acgcagaaca cgctgcgcca gcgccagttc
   102481 gccgtacagc tgataacggg tctgcgggcg ctttttacgc tccataactt tggtccagac
   102541 gcgtttcaga taagcggcat cggaggccag ttcagcctcg ccaacccctt ccgctgcagt
   102601 acggatgata aacccgccct gctcgtcgca atactccgcg accacttttt tcaggcgttc
   102661 acgttctgat tcgctttcaa tacgttggga aacaccaacg tgagaagccc ctggcataaa
   102721 gaccagatag cgagaaggca gcgtgatatc ggtggtcagg cgcgccccct tagtgccaag
   102781 cggatctttc accacctgca ccatcagatc ttgcccctga cgaaccagtt ccgagatgtc
   102841 gcgcaccgtg aattgctttt gttcttcacc cgccacacat tcggtgtgcg gcatgatgtc
   102901 agatgcatga agaaacgcgg ctttatccag cccaatatct acaaaagccg cctgcatacc
   102961 cggaagtaca cgacttacac gacccttgta gatattgcct actattccgc gtcgcgcctc
   103021 acgttcaata tgaatttcct gcagaatacc gccatcaata tacgccactc gcgtttccga
   103081 aggcgttacg tttactaaca attcagccgt catgtttatc ccttttctca cgcagtgcgt
   103141 taaaattact taataattca tacgtttcaa ccagcggtaa gccgactacg gcgtgatagc
   103201 tgccatttat cttcctgaca aaacagccac ccagcccctg aataccgtat gcacctgctt
   103261 tatctaacgg ttcaccgctg gcgacatagc ccgcgatgtc ttcgtctgtt aacgttctga
   103321 aagtcacatc ggtgaccacc aggcaatcga gaatgtgctg gctgtcggcc aacgccactg
   103381 ccgtcatcac ctgatgggtc tgacccgata atttgcgcaa catctgcgcc gcatgctctg
   103441 cgtcgcgcgg tttctccagc acttctccgt tcaggataac gatagtatcc gcacccagca
   103501 ccgggagatc ctgcgccgtt tgcgtgacac ccgcccgtgc tttctcgcgc gccagacgca
   103561 caacatactg ctgcgcgctc tcctgcggct gacgctgctc ctcaatgccc gtaacaatac
   103621 gttcaaaggt cacgccaagt tgcgcaagta actcctgacg acgcggagaa ccggaagcta
   103681 aatacagaga agtcatagat acctttattg cactgcaaac tgctgacgga ctttacgcat
   103741 cagcaagaaa atccacggcc agagcacccc attgactaca ctactccaga acacttccgg
   103801 tctgaaagag acgttaatca ctaaaaactc tgcccagaaa acaataatat ccaccaccag
   103861 cgaaagcaac atgacgacca gcgcctgctg ccataatgcg aggttgcgga aaagctggta
   103921 tttcagcgcc accaggtaag caatgatgct catcgccaat acgcgtacgc caagcgtcga
   103981 gccgctgatc agatccagta tggcacccat cacaaaacct gtgcccacat ttacgcgatg
   104041 gggcaaggcc aggatccaat acaacaagat gagtaacacc cagtttggcc ggaaaacaat
   104101 caggttatcc ggccagggca tgatttgcag caaaagcgca atgaggaaag agagccagat
   104161 tacccagcgt ccctggctac gatagctcgc cactattgcc ctcccggcgc acgcgcaggc
   104221 ggttgagcac cactttgcgg cggcgtagcc ctttgtggag agcgattagc agcgggctgt
   104281 gtcggcgcag caggcgcagt agctgtattt cctgtcgtgg gttgctgcgg agtcggctga
   104341 gcgatccccg ttgccggttc aggtaacttt ggccccatcg cgtctggcga aggcaatacc
   104401 tgcggcatca tctgcatcag acgttcatta gcaacacgat gcacctcttc cggcgtcatc
   104461 gggttagcgc cgttacgatc tgccccccac agcagcagca gataacgcaa acgttgcagc
   104521 cctgcagtcg gacgcgcctg aatcacagta taagcgcgct gggtatcgag ttttacggaa
   104581 gagacaaccg cgaccggata gccttccggg aaacgaccgc ccagaccgga agtcaccagc
   104641 acatcaccaa cacgaatatc cgtattcgcc ggcagatgct caagctgcaa atcatccgta
   104701 caaccgttac cggctgcaat tacgcggata tcgttgcgca gcacctggat tggcagcgcg
   104761 tgggtcgcat cacaaatcag cagcacgcga ctggtcagtt tagcgacggc caccacctga
   104821 ccaacaacac ctttgtcgct gatgaccggc tggccttcat aaacgccatt aacgctacct
   104881 ttatcgataa caacttgatc gctataagga tcgttaaccg tggagataac ctgagtcacc
   104941 attttctgct catcctgacg cagcggggaa cccagcagct cgcgcagacg cgcgttctcc
   105001 tgtttgtatt gtccaagcat cagcagttca ctgtttttca gcaacagttc ctgacgtaac
   105061 gcccggtttt caagttctag ttggtcacgc gaggccagcg tctgcgatac gccatccagc
   105121 aattcacgag gagcattgga aacaaagtag aaaggactga cggcggtatc catataagta
   105181 cggatttgac tgaacgtccc caggcggctg tcggcaataa taatgccgag cgccaccagc
   105241 accgccagaa taaggcgaat ctgtagcgac gggccacggc taaaaattgg cttcataagt
   105301 tatgcgtatt ctcgtatcag accaggcagg gtaaacagac gcttcccctg cctgcatccg
   105361 attactcttc gctgaacagg tcgccgccgt gcatgtcgat catttccagc gctttgccgc
   105421 caccgcgcgc cacacaggtc agcgggtctt cagcaacaac gactggaatg ccggtttctt
   105481 ccattaacaa acggtcaagg ttacgcagca gtgcgccacc accggtgagc accatgccgc
   105541 gctcggagat gtcggaagcc agttccggcg ggcactgttc cagtgcaacc attaccgcgc
   105601 tcacaatacc ggtcagcggt tcctgcagtg cttcgaggat ttcgttggag ttcagggtaa
   105661 aaccgcgtgg aacaccttct gccaggttac ggccacgaac ttcgatttca cggacttcat
   105721 cgcccggata agccgaaccg atttcgtgct tgatacgttc tgcggtggct tcaccgatca
   105781 gagaaccgta attacgacgc acatagttga tgatagcttc gtcgaaacgg tcaccaccaa
   105841 tgcgtacgga agaggagtaa accacgccgt tcaaggagat aacagcaact tcagtggtac
   105901 caccaccgat atcaaccacc atagaaccgg tcgcttcaga aaccggcagg ccagcaccaa
   105961 ttgcggcagc catcggttct tcaatcagga agacttcacg ggcaccagcg ccctgcgcgg
   106021 attcacgaat tgcgcggcgt tcaacctggg tcgcgccaac cggtacacaa accagaacgc
   106081 gcgggcttgg acgcataaag ctgttgctgt gcacttgttt gatgaagtgc tggagcattt
   106141 tttcagtcac gaagaagtcg gcgataacgc cgtctttcat tgggcgaatg gcagcaatat
   106201 tgcccggcgt acggcccagc atctgcttcg cgtcatgacc tactgcagct acgcttttcg
   106261 gtgaaccggc acgatcctga cgaatggcca ccacggaagg ctcattcaat acgatgcctt
   106321 gtccttttac ataaatgagg gtattcgcag tacccaggtc aatggacaag tcattggaaa
   106381 acatgccacg aaattttttc aacatactaa gggataatcc tgaaagctgg ggcggaaaag
   106441 aaaatccgct tactttacca accacacgca gcagcgacaa ggcgcaaaaa tcatctgcta
   106501 cggtgaaaat tagtgcagtt cgtttccttt gttacaaatc tctgcctgag tccagaaagg
   106561 cttaatgcat cagcagcatt cctcgcctgt ttgcaaccgc gtaaggtcat tcatctgcat
   106621 atgtgctgca acaatctggc gagcagacaa gcacactccc atgagacgca gcgcgcgtta
   106681 ttctacgtga aaacggatta aacggcaggt taaaccgagt atctttgtga atattttttc
   106741 acgttagtat caagtggctg tgaggacgcg aaaaaatccc cttgcccgcc tgtaacaccg
   106801 cgctgaatca gggtctgcca ctcgcttcgc gaacgcacac cggtggcgta aacctgggtg
   106861 ctggtccctg agcaggcttc caccaggctt tgaaccagca gctggttctc cgttcgcttc
   106921 tcaatgtttc tgaccagccc cggatggagc ttgagtaact caacattaag ttctttgatc
   106981 caactggtgc ttaccagcgt caaaccagcc tggttgacgg ctacccgtac ccctaaagca
   107041 ttcactaaac gaataacagg ttgtaaacga ctgatatgtt gacctacatc ggcctctgca
   107101 agttcaataa ttatgcgttt tcgttgtgat ttttcacatt gcattaacgt atcgcgcagc
   107161 caacgctgaa aacgcgggcg aatcagcgac tcaacggtaa cctgaatcgc cagattttcc
   107221 tctggccagt aacgcaatag tggaataaga cggctgattt gcagacggtc atactcttcc
   107281 gataagccaa actgcaagac catcggcata tactccgccg agctaacctc ttcattacca
   107341 tcaaagatgc ggcacatgag ttcgcgatga tgaacctgac cttcgcgagt aaccgccggt
   107401 ttttgataaa ggcgcgggcc gccgcgactg agcatttgct cgataagcgt acgccagcga
   107461 acattaccgc gtcctttttc aggcaacgag tcatcgtaaa tagcccagct attgccgccc
   107521 tgcaatcccg cattacgcgt ggcagactct gcatgttcca ttacctgctc ggtatcctga
   107581 ccactacgcc aggcgcagat accaatgtgg atcatatcgt cgcgatcgag cattttattg
   107641 ttcggcaagg tatcgacggc tttgattaac tgaccggcga tgctctctgc ctcttttaac
   107701 gtccggtgcg gtaacagcgc agcaaaatca ctgcggtggt aacgcgccag cagtgcgcca
   107761 gggtagcgca tcataaatgt cgacagcaga ttcgtcagag tgaagaactg ttcttcaacc
   107821 tggctgtgcc cccaggtatc gctcaacata ttgaaatccg gcagacgaat catcatcacg
   107881 atcccgtggg tacctacttt ctcctgatct tccagtaacg ttgctaactg attatcgaaa
   107941 aagagtcggt tattgaggcc ggttttcacg tcctgtgcgg cataagagcg gatcagcgta
   108001 tcaagacggc tgtgttgttc gcgtgcgttc tgaatttcac gaagcagcgt atccagcgca
   108061 ctgctggttg tgggcggcca ttcatagatg gttcccaaca cattagagcc acgctcaccg
   108121 tttaagatac gagtagcccg ggtttccagc aattcttgcc cggcaagttg ccgttgtaac
   108181 cagcgtaccg ccagaaagag cataagaatg ataaagccaa tcgcccccgt gagcggcgcg
   108241 gtggtcatca acgaatggaa atagttgccc atcggatcct gataaaccag acgcaacgac
   108301 atccccggat gctttatcaa cggaacgctc agttcacgaa acagatcgct ggtgccaacc
   108361 ggacgataac taccatttct ggccagggta taaacctgtt tatcaccatg cagcaggtct
   108421 acacgaacga tatctgctga caccattaat tcggtaattt gtggccttaa tgcgctgaag
   108481 tcattcgaca caaggtgggt atcaatcgcc gtcgccaccg cctgaacgcg atgactaaac
   108541 ttatactgaa tggcgttgta gaaacttagc gaacagccca gcaaagtcac aaaaattgtt
   108601 aacccggtga gcagcgtaac aaaggccgaa aatttcgtcg ttaatctcat ccttgtgtta
   108661 actccgatag tgaggaagcg ggcatactag caaatcagat ttatctcgca atttattgcg
   108721 cttcatcggc tttgcttttc cattagcgag tatagtcttc agaaattatt ttccaatcca
   108781 tcatgcacat gaggaccact tatgcaggcg ttacttttag aacagcagga cggcaaaact
   108841 ctcgcatcag tacagactct ggacgaaagt cgcctgccgg agggcgatgt cacggtcgat
   108901 gttcactggt cgagcctgaa ctataaagat gcgctggcga ttaccggtaa gggaaaaatc
   108961 atccgtaatt ttccgatgat tcctgggatc gattttgccg gaactgtacg caccagcgaa
   109021 gatccgcgtt ttcatgccgg tcaggaggtg ttactcactg gctggggcgt tggtgaaaac
   109081 cactggggtg ggctggcgga gcaggcgcga gtgaaaggtg actggctggt tgccatgccg
   109141 caagggctgg acgcgcgtaa agcaatgatt atcggtactg ccggttttac cgccatgctg
   109201 tgtgtgatgg cgctggaaga tgccggtgtt cgcccgcagg acggggagat tgtcgtgacg
   109261 ggtgccagtg gtggcgtcgg cagtaccgcc gtggcgctgc tgcataagtt gggttatcag
   109321 gtcgttgccg tttccggtcg cgaaagtacc catgaatatc tgaaaagttt aggtgccagc
   109381 cgtattctcc ctcgtgatga gtttgccgaa tcccgtcctc tggaaaaaca agtctgggct
   109441 ggggcaattg acactgttgg cgacaaagtg ctggcaaaag tgctggcgca aatgaattac
   109501 ggcggctgcg tggcggcctg tggtctggcg ggtggtttta ctctgccaac cacggtcatg
   109561 ccatttattc tgcgtaatgt ccgtttgcaa ggggtggatt cagtaatgac gccaccagaa
   109621 cgccgcgcac aagcctggca gcgactggtc gccgatttac cggaatcatt ctatacccag
   109681 gcggcaaaag agatatctct gtcagaggca ccgaactttg ccgaggccat cattaataac
   109741 cagatccagg gtcgcacgct ggtgaaggtt aactaaccat ttagcaagga ataataagag
   109801 agggaactca ttttgaaatc attattcaat cggttaacgg gaaaagcggt tagccggaca
   109861 gctttcgtcg aacaccttgg tcatgaagtt gtacaacatc atccaaactg gaaagtcatg
   109921 atttcgactg accataaatt gatgcgcatt gatactccac taaacagcta ttattgatac
   109981 gcctccgtcg cctgttaggt ttatggtgct ttgcctgggc gacgctacgc ttaacctgtt
   110041 actgatttct ggtaccatgg ggtgaataat ctgattttat ttggctacaa attaaccact
   110101 cgaacctatt taatgctgag cattgtcagt cggttaattt tgcgtgcttt agcattcaca
   110161 tctatccaga cgatgcagtg aaaattgggt aatccccagc aaccgctgcg taatgtcgtc
   110221 tatcttgtcg cgatcctggc atccctacat tatttgtggt ctgtgaagat tatctcattg
   110281 cagcccctca tcttcgcagg gctggcttta cagcttttca ccttacgtta taagaagttc
   110341 cgtcgatgat ggcgctaatt tcgtgaattg tgcggcttgt tgcaaattac acggtgttga
   110401 aggttattta catgttagct gttgattatc ttccctgata agaccagtat ttagctgcca
   110461 attgctacga aatcgttata atgtgcgacc tcgtcctccc tgacgcagtt tttgcgctgc
   110521 ggaaaaggtg acattggcgc aacgaaggta tattttgttt tttgccggag gatagcagca
   110581 gatcgctgca caatgtccgt caagtctaac attgacactc tggggcaaaa tagaccggcg
   110641 tcccggcctg ctggaattta tcgctatgca tacagctgtc ggggcatacg ctttacagac
   110701 ggcggtgaaa cgcctgtcac aatcacacta aacaaagagt acggaaccca ctcatggata
   110761 ttcgtaagat taaaaaactg atcgagctgg ttgaagaatc aggcatctcc gaactggaaa
   110821 tttctgaagg cgaagagtca gtacgcatta gccgtgcagc tcctgccgca agtttccctg
   110881 tgatgcaaca agcttacgct gcaccaatga tgcagcagcc agctcaatct aacgcagccg
   110941 ctccggcgac cgttccttcc atggaagcgc cagcagcagc ggaaatcagt ggtcacatcg
   111001 tacgttcccc gatggttggt actttctacc gcaccccaag cccggacgca aaagcgttca
   111061 tcgaagtggg tcagaaagtc aacgtgggcg ataccctgtg catcgttgaa gccatgaaaa
   111121 tgatgaacca gatcgaagcg gacaaatccg gtaccgtgaa agcaattctg gtcgaaagtg
   111181 gacaaccggt agaatttgac gagccgctgg tcgtcatcga gtaacgaggc gaacatgctg
   111241 gataaaattg ttattgccaa ccgcggcgag attgcattgc gtattcttcg tgcctgtaaa
   111301 gaactgggca tcaagactgt cgctgtgcac tccagcgcgg atcgcgatct aaaacacgta
   111361 ttactggcag atgaaacggt ctgtattggc cctgctccgt cagtaaaaag ttatctgaac
   111421 atcccggcaa tcatcagcgc cgctgaaatc accggcgcag tagcaatcca tccgggttac
   111481 ggcttcctct ccgagaacgc caactttgcc gagcaggttg aacgctccgg ctttatcttc
   111541 attggcccga aagcagaaac cattcgcctg atgggcgaca aagtatccgc aatcgcggcg
   111601 atgaaaaaag cgggcgtccc ttgcgtaccg ggttctgacg gcccgctggg cgacgatatg
   111661 gataaaaacc gtgccattgc taaacgcatt ggttatccgg tgattatcaa agcctccggc
   111721 ggcggcggtg gtcgcggtat gcgcgtagtg cgcggcgacg ctgaactggc acaatccatc
   111781 tccatgaccc gtgcggaagc gaaagctgct ttcagcaacg atatggttta catggagaaa
   111841 tacctggaaa atcctcgcca cgtcgagatt caggtactgg ctgacggtca gggcaacgct
   111901 atctatctgg cggaacgtga ctgctccatg cagcgccgcc accagaaagt ggtcgaagaa
   111961 gcaccagcac cgggcattac cccggaactg cgtcgctaca tcggcgaacg ttgcgctaaa
   112021 gcgtgtgttg atatcggcta tcgcggtgca ggtactttcg agttcctgtt cgaaaacggc
   112081 gagttctatt tcatcgaaat gaacacccgt attcaggtag aacacccggt tacagaaatg
   112141 atcaccggcg ttgacctgat caaagaacag ctgcgtatcg ctgccggtca accgctgtcg
   112201 atcaagcaag aagaagttca cgttcgcggc catgcggtgg aatgccgtat caacgccgaa
   112261 gatccgaaca ccttcctgcc aagcccgggc aaaatcaccc gtttccacgc acctggcggt
   112321 tttggcgtac gttgggagtc tcatatctac gcgggctaca ccgtaccgcc gtactatgac
   112381 tcaatgatcg gtaagctgat ttgctacggt gaaaaccgtg acgtggcgat tgctcgcatg
   112441 aaaaatgcgc tgcaggagct gatcatcgac ggtatcaaaa ccaacgttga tctgcagatc
   112501 cgcatcatga atgacgagaa cttccagcat ggtggcacta acatccacta tctggagaaa
   112561 aaactcggtc ttcaggaaaa ataagactgc aaaagcgtca aaaggccgga ttttccggcc
   112621 ttttttatta ctggggatcg acaaccccca taaggtacaa tcctcgcttt cttcacccat
   112681 cagggacaaa aaatggacac tcgttttgtt caggcccata aagaggcgcg ctgggcgctg
   112741 gggctgaccc ttttgtatct ggcagtttgg ttagtagccg cttacttacc tggcgttgcc
   112801 cccggtttta ccggctttcc gcgctggttt gagatggcct gcatcctgac gccgctgctg
   112861 tttattggac tgtgctgggc gatggtgaaa tttatctatc gcgatatccc actggaggat
   112921 gacgatgcag cttgaagtaa ttctaccgct ggtcgcctat ctggtggtgg tgttcggtat
   112981 ctctgtttat gcgatgcgta aacggagcac cggcaccttc cttaatgagt atttcctcgg
   113041 cagccgctct atgggcggta ttgtgctggc gatgacgctc accgcgactt atatcagtgc
   113101 cagttcgttt atcggcgggc ctggagctgc ttacaaatac ggccttggct gggtattgct
   113161 ggcgatgatt cagcttcctg cagtctggct ttcactcggt attctcggca agaagttcgc
   113221 gattctcgcg cgtcgctaca atgcggtaac gctgaacgat atgctatttt cccgctacca
   113281 gagtcgtctt ttggtgtggc tggcgagttt gagtttgctg gtcgcgttta ttggcgcgat
   113341 gaccgtgcag tttatcggcg gcgcgcgcct gctggaaacc gcggcgggta ttccttatga
   113401 aaccgggctg ctgattttcg gtatcagcat tgcgttgtat accgcctttg gtggctttcg
   113461 cgccagcgtg ctgaacgaca ccatgcaagg gcttgtgatg ctgattggca ccgttgtgct
   113521 gcttattggc gtggtacatg ccgctggcgg cttaagcaac gcagtacaga ccttgcaaac
   113581 catcgatccg caactggtta cgccacaagg cgctgacgat attctgtcgc ctgcctttat
   113641 gacgtcgttc tgggtactgg tgtgctttgg cgttataggc ctgccgcata ctgcggtgcg
   113701 ctgtatctct tataaagaca gcaaagcagt acaccggggg atcatcatcg gtaccattgt
   113761 agtcgcaatc ctgatgttcg gtatgcacct ggccggagcg ttaggtcgtg cagtgatccc
   113821 cgatctcacc gtaccggatc tggtgatccc gacgttaatg gtaaaagtgc tgccaccgtt
   113881 tgctgccggg atctttctgg ctgcacctat ggctgcgatc atgtcgacaa ttaacgccca
   113941 attgctacaa agttccgcta cgatcattaa agatctctat ctgaatatcc gtccggatca
   114001 aatgcaaaac gagacgcgtc tgaagcggat gtcggcggta attacgttag ttctcggcgc
   114061 gttgctgctg cttgccgcct ggaagccgcc agaaatgatc atctggctga atttgttggc
   114121 cttcggtggg ctggaagccg ttttcctgtg gccgctggtg ctgggtcttt actgggaacg
   114181 cgccaacgcc aaaggcgcgc taagtgcgat gatcgttggc ggcgtgctgt atgccgtact
   114241 cgcgacgctg aatattcagt acctgggctt ccaccctatc gtgccctcgt tactactaag
   114301 tttgctggct ttcctggtcg gaaaccgttt cggtacatcc gtcccgcaag ctaccgtttt
   114361 gactactgat aaataaagag ttttgccatg ccttggatcc aactgaaact gaacaccacc
   114421 ggcgcgaatg cggaagatct tagcgatgcg ctgatggaag cgggtgccgt ttctatcact
   114481 tttcaggaca cccacgatac gccagtattt gagccgctgc cgggcgaaac gcgcctgtgg
   114541 ggcgacaccg atgtgattgg tctgttcgac gctgaaaccg acatgaacga cgtggtggcg
   114601 attctggaaa accatccgct gctcggcgca ggcttcgcgc ataaaatcga acaactggaa
   114661 gataaagact gggagcgcga atggatggat aatttccacc cgatgcgctt tggtgaacga
   114721 ctgtggatct gcccaagctg gcgtgatgtg ccggacgaaa acgccgtcaa cgtgatgtta
   114781 gaccccgggc tggcgtttgg tacgggtacc catccaacta cctctctgtg cctgcaatgg
   114841 ctcgacagcc tcgatttaac cggtaaaaca gtcatcgact ttggctgtgg ttccggcatt
   114901 ctggcgatcg cggcgctgaa actgggtgca gcaaaagcca ttggtattga tatcgatccg
   114961 caggcgattc aggccagccg cgataacgcc gaacgtaatg gcgtttctga ccgtctggaa
   115021 ctctacttac cgaaagatca gccagaagaa atgaaagctg acgtggtgat cgctaacatc
   115081 cttgcaggcc cattacgtga actggcaccg ttaatcagcg tcctgccggt ttcaggcggt
   115141 ttgctgggcc tttccggtat tctggcaagc caggcagaga gcgtttgtga agcttatgcc
   115201 gacagcttcg cactggaccc ggtcgtggaa aaagaagagt ggtgccgtat taccggtcgt
   115261 aagaattaac cttcgcatcg ccgtagggtg acgcggggcg aagtgcgagc aagctcacaa
   115321 aaggcacgta aatttgccga ttatttacgc aaatttgcgt gccaaaattt tcattcataa
   115381 agaaaaattg agaacttact caaatttctt tgagtgtaaa ttttagtcac tattttctaa
   115441 tatgatgatt tttatgagta attatcgcgc tacgctcatt ttaaatgcaa ttctttgatc
   115501 catctcagag gattggtcaa agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac
   115561 gccgccttgc agtcacagta tggtcatttc ttaactcatg cgcatcggac aatatcagct
   115621 cagaaatcgc ctgatcgcag cgcccatggc tggcattaca gacagacctt ttcggacgtt
   115681 gtgctacgag atgggagccg gattgacagt atccgagatg atgtcttcta acccacaggt
   115741 ttgggaaagc gacaaatctc gtttacggat ggtgcacatt gatgaacccg gtattcgcac
   115801 cgtgcaaatt gctggtagcg atccgaaaga aatggcagat gcagcacgta ttaacgtgga
   115861 aagcggtgcc cagattattg atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa
   115921 gctcgcaggt tcagccctct tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt
   115981 cgtcaatgca gtggacgttc ctgttaccct gaagattcgc accggctggg caccggaaca
   116041 ccgtaactgc gaagagattg cccaactggc tgaagactgt ggcattcagg ctctgaccat
   116101 tcatggccgt acacgcgcct gtttgttcaa tggagaagct gagtacgaca gtattcgggc
   116161 agttaagcag aaagtttcca ttccggttat cgcgaatggc gacattactg acccgcttaa
   116221 agccagagct gtgctcgact atacaggggc ggatgccctg atgataggcc gcgcagctca
   116281 gggaagaccc tggatctttc gggaaatcca gcattatctg gacactgggg agttgctgcc
   116341 cccgctgcct ttggcagagg ttaagcgctt gctttgcgcg cacgttcggg aactgcatga
   116401 cttttatggt ccggcaaaag ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca
   116461 ggaacacgct cccaatgacc agtttcggcg cacattcaac gccattgagg atgccagcga
   116521 acagctggag gcgttggagg catacttcga aaattttgcg taaacagaaa taaagagctg
   116581 acagaactat gttcgaacaa cgcgtaaatt ctgacgtact gaccgtttct accgttaact
   116641 ctcaggatca ggtaacccaa aaacccctgc gtgactcggt taaacaggca ctgaagaact
   116701 attttgctca actgaatggt caggatgtga atgacctcta tgagctggta ctggctgaag
   116761 tagaacagcc cctgttggac atggtgatgc aatacacccg tggtaaccag acccgtgctg
   116821 cgctgatgat gggcatcaac cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga
   116881 actaattcag gttagctaaa tgcttgatta aaaaggcgct actcggcatg gggaagcgcc
   116941 ttttttatta aaatgactgc accactgact gcacataagc acgaacgaaa ccgaacaata
   117001 ataaatagaa tagatacctg ccagtggtta tgtctccgca gtgatgtgtt ttttcatttt
   117061 taatggggat aaaaacggtg tggtgagcgg tccggaagac gccatggcaa gagttttgcc
   117121 ccgcttctcc ctgtgcatga tagataaccg agggcggaat tccgccttcg gtgctcacat
   117181 atgagaaata atatttcgtc caccgatacc ctttatttgg gtatcgcgag taagccccag
   117241 aatctgggtg ttactcttac tacccaaact acgggtagtt tccgtagttt catggtcgag
   117301 ttgcagatct gcaactcacc caccagccaa cccattttgg ggctgtcggg aatatcaacc
   117361 agttactgac aatgctctgg gcgttctgtc tagctggtgg ctgtggctgg acgctaattt
   117421 ttgcgtacca cttttgcgca ctttttcagg gtcggatttc agatccccac cattctggga
   117481 gcagttgcag gggccgaata aatcaatagc cgtgctggta acgttttgca tgacaagttt
   117541 tgacagtttt ttatcttccg gcctcctcaa aattgaggat tgaagaaaag tcaatgggtt
   117601 aggtcatcgt gcaaatttgc actatgccag ccaacgcaat tttgcgttat cgggaatatc
   117661 agcaagttac cgccgcagtc gttccggctt cttccactgg taagtatttt tcgcgctctc
   117721 cctccgttgt tgagaacggc gacggtatgc cagcaactca aggactctgg ttcgtatgtt
   117781 gcgcatatcc acgccgttaa gctcaatacc gtcacggcgc atcacctcag ccaccacacg
   117841 cgcgtaattt tcggctatca cgctgtccgg ctgtgtagcc tgttcgtcag gctgctgact
   117901 gattccggta atgcggcgaa ttatttttag tagatcagtt tcagtcattc tcgtcatacc
   117961 ccatcaaacg ccgcaagtcg ctctttgtgg ctgtcgctca tatc
//
LOCUS       DAZFFG010000004       104426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFFG010000004 DAZFFG010000000
VERSION     DAZFFG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 104426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.004"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            479..1567
                     /gene="yehL"
                     /locus_tag="ACHJ7J_000404"
     CDS             479..1567
                     /gene="yehL"
                     /locus_tag="ACHJ7J_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO4998686.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDAAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            1578..3857
                     /gene="yehM"
                     /locus_tag="ACHJ7J_000405"
     CDS             1578..3857
                     /gene="yehM"
                     /locus_tag="ACHJ7J_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO4998687.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIGWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQSWCWQWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHKLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DDDFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            3850..4986
                     /gene="yehP"
                     /locus_tag="ACHJ7J_000406"
     CDS             3850..4986
                     /gene="yehP"
                     /locus_tag="ACHJ7J_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4998688.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGSSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            4983..6985
                     /gene="yehQ"
                     /locus_tag="ACHJ7J_000407"
                     /pseudo
     CDS             4983..6985
                     /gene="yehQ"
                     /locus_tag="ACHJ7J_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
     gene            7110..7571
                     /gene="yehR"
                     /locus_tag="ACHJ7J_000408"
     CDS             7110..7571
                     /gene="yehR"
                     /locus_tag="ACHJ7J_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO4998689.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(7612..8082)
                     /gene="yehS"
                     /locus_tag="ACHJ7J_000409"
     CDS             complement(7612..8082)
                     /gene="yehS"
                     /locus_tag="ACHJ7J_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO4998690.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(8129..8848)
                     /gene="btsR"
                     /locus_tag="ACHJ7J_000410"
                     /gene_synonym="yehT"
     CDS             complement(8129..8848)
                     /gene="btsR"
                     /locus_tag="ACHJ7J_000410"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO4998691.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(8845..10530)
                     /gene="btsS"
                     /locus_tag="ACHJ7J_000411"
     CDS             complement(8845..10530)
                     /gene="btsS"
                     /locus_tag="ACHJ7J_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO4998692.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            10752..11483
                     /gene="mlrA"
                     /locus_tag="ACHJ7J_000412"
     CDS             10752..11483
                     /gene="mlrA"
                     /locus_tag="ACHJ7J_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO4998693.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            11543..11650
                     /gene="yohO"
                     /locus_tag="ACHJ7J_000413"
     CDS             11543..11650
                     /gene="yohO"
                     /locus_tag="ACHJ7J_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO4998694.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(11631..12362)
                     /gene="yehW"
                     /locus_tag="ACHJ7J_000414"
     CDS             complement(11631..12362)
                     /gene="yehW"
                     /locus_tag="ACHJ7J_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO4998695.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(12367..13293)
                     /gene="yehX"
                     /locus_tag="ACHJ7J_000415"
     CDS             complement(12367..13293)
                     /gene="yehX"
                     /locus_tag="ACHJ7J_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO4998696.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(13286..14443)
                     /gene="yehY"
                     /locus_tag="ACHJ7J_000416"
     CDS             complement(13286..14443)
                     /gene="yehY"
                     /locus_tag="ACHJ7J_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO4998697.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(14450..15367)
                     /gene="osmF"
                     /locus_tag="ACHJ7J_000417"
     CDS             complement(14450..15367)
                     /gene="osmF"
                     /locus_tag="ACHJ7J_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO4998698.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(15619..17916)
                     /gene="bglX"
                     /locus_tag="ACHJ7J_000418"
     CDS             complement(15619..17916)
                     /gene="bglX"
                     /locus_tag="ACHJ7J_000418"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO4998699.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPMDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANITSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            18112..19827
                     /gene="dld"
                     /locus_tag="ACHJ7J_000419"
     CDS             18112..19827
                     /gene="dld"
                     /locus_tag="ACHJ7J_000419"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4998700.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPELDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(19866..20798)
                     /gene="pbpG"
                     /locus_tag="ACHJ7J_000420"
     CDS             complement(19866..20798)
                     /gene="pbpG"
                     /locus_tag="ACHJ7J_000420"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO4998701.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(20972..21559)
                     /gene="yohC"
                     /locus_tag="ACHJ7J_000421"
     CDS             complement(20972..21559)
                     /gene="yohC"
                     /locus_tag="ACHJ7J_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO4998702.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            21730..22308
                     /gene="yohD"
                     /locus_tag="ACHJ7J_000422"
     CDS             21730..22308
                     /gene="yohD"
                     /locus_tag="ACHJ7J_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4998703.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(22438..23199)
                     /gene="yohF"
                     /locus_tag="ACHJ7J_000423"
     CDS             complement(22438..23199)
                     /gene="yohF"
                     /locus_tag="ACHJ7J_000423"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4998704.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(23252..24688)
                     /gene="mdtQ"
                     /locus_tag="ACHJ7J_000424"
     CDS             complement(23252..24688)
                     /gene="mdtQ"
                     /locus_tag="ACHJ7J_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO4998705.1"
                     /translation="MNRDSFYPAIACFPLLLMLTGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKALAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            24912..24995
                     /gene="yohP"
                     /locus_tag="ACHJ7J_000425"
     CDS             24912..24995
                     /gene="yohP"
                     /locus_tag="ACHJ7J_000425"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO4998706.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(25382..26332)
                     /gene="dusC"
                     /locus_tag="ACHJ7J_000426"
     CDS             complement(25382..26332)
                     /gene="dusC"
                     /locus_tag="ACHJ7J_000426"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO4998707.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSHDIARAIQAIDIDKLR"
     gene            complement(26492..27685)
                     /locus_tag="ACHJ7J_000427"
     CDS             complement(26492..27685)
                     /locus_tag="ACHJ7J_000427"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO4998708.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(27700..28344)
                     /gene="maiA"
                     /locus_tag="ACHJ7J_000428"
     CDS             complement(27700..28344)
                     /gene="maiA"
                     /locus_tag="ACHJ7J_000428"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO4998709.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(28353..29054)
                     /locus_tag="ACHJ7J_000429"
     CDS             complement(28353..29054)
                     /locus_tag="ACHJ7J_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4998710.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(29070..30098)
                     /gene="gtdA"
                     /locus_tag="ACHJ7J_000430"
     CDS             complement(29070..30098)
                     /gene="gtdA"
                     /locus_tag="ACHJ7J_000430"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO4998711.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(30110..31468)
                     /locus_tag="ACHJ7J_000431"
     CDS             complement(30110..31468)
                     /locus_tag="ACHJ7J_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4998712.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            31595..32503
                     /locus_tag="ACHJ7J_000432"
     CDS             31595..32503
                     /locus_tag="ACHJ7J_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4998713.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            32634..33032
                     /gene="yohJ"
                     /locus_tag="ACHJ7J_000433"
     CDS             32634..33032
                     /gene="yohJ"
                     /locus_tag="ACHJ7J_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO4998714.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            33029..33724
                     /gene="yohK"
                     /locus_tag="ACHJ7J_000434"
     CDS             33029..33724
                     /gene="yohK"
                     /locus_tag="ACHJ7J_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO4998715.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            33854..34738
                     /gene="cdd"
                     /locus_tag="ACHJ7J_000435"
     CDS             33854..34738
                     /gene="cdd"
                     /locus_tag="ACHJ7J_000435"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO4998716.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            34888..35607
                     /gene="sanA"
                     /locus_tag="ACHJ7J_000436"
     CDS             34888..35607
                     /gene="sanA"
                     /locus_tag="ACHJ7J_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO4998717.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFSTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            35610..35849
                     /gene="yeiS"
                     /locus_tag="ACHJ7J_000437"
     CDS             35610..35849
                     /gene="yeiS"
                     /locus_tag="ACHJ7J_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO4998718.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            36043..37280
                     /gene="preT"
                     /locus_tag="ACHJ7J_000438"
                     /pseudo
     CDS             36043..37280
                     /gene="preT"
                     /locus_tag="ACHJ7J_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            37274..38509
                     /gene="preA"
                     /locus_tag="ACHJ7J_000439"
     CDS             37274..38509
                     /gene="preA"
                     /locus_tag="ACHJ7J_000439"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO4998719.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(38752..39762)
                     /gene="mglC"
                     /locus_tag="ACHJ7J_000440"
     CDS             complement(38752..39762)
                     /gene="mglC"
                     /locus_tag="ACHJ7J_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO4998720.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(39778..41298)
                     /gene="mglA"
                     /locus_tag="ACHJ7J_000441"
     CDS             complement(39778..41298)
                     /gene="mglA"
                     /locus_tag="ACHJ7J_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO4998721.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGSILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(41359..42357)
                     /gene="mglB"
                     /locus_tag="ACHJ7J_000442"
     CDS             complement(41359..42357)
                     /gene="mglB"
                     /locus_tag="ACHJ7J_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO4998722.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(42637..43677)
                     /gene="galS"
                     /locus_tag="ACHJ7J_000443"
     CDS             complement(42637..43677)
                     /gene="galS"
                     /locus_tag="ACHJ7J_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO4998723.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            complement(43819..44976)
                     /gene="yeiB"
                     /locus_tag="ACHJ7J_000444"
     CDS             complement(43819..44976)
                     /gene="yeiB"
                     /locus_tag="ACHJ7J_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO4998724.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDTQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(44993..45661)
                     /gene="folE"
                     /locus_tag="ACHJ7J_000445"
     CDS             complement(44993..45661)
                     /gene="folE"
                     /locus_tag="ACHJ7J_000445"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO4998725.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            45919..46755
                     /gene="yeiG"
                     /locus_tag="ACHJ7J_000446"
     CDS             45919..46755
                     /gene="yeiG"
                     /locus_tag="ACHJ7J_000446"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4998726.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(46787..48766)
                     /gene="cirA"
                     /locus_tag="ACHJ7J_000447"
     CDS             complement(46787..48766)
                     /gene="cirA"
                     /locus_tag="ACHJ7J_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO4998727.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(49058..50527)
                     /gene="lysP"
                     /locus_tag="ACHJ7J_000448"
     CDS             complement(49058..50527)
                     /gene="lysP"
                     /locus_tag="ACHJ7J_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO4998728.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(50732..51613)
                     /gene="yieE"
                     /locus_tag="ACHJ7J_000449"
     CDS             complement(50732..51613)
                     /gene="yieE"
                     /locus_tag="ACHJ7J_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO4998729.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            51712..52761
                     /gene="yeiH"
                     /locus_tag="ACHJ7J_000450"
     CDS             51712..52761
                     /gene="yeiH"
                     /locus_tag="ACHJ7J_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO4998730.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            52835..53692
                     /gene="nfo"
                     /locus_tag="ACHJ7J_000451"
     CDS             52835..53692
                     /gene="nfo"
                     /locus_tag="ACHJ7J_000451"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO4998731.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            53695..54783
                     /gene="yeiI"
                     /locus_tag="ACHJ7J_000452"
     CDS             53695..54783
                     /gene="yeiI"
                     /locus_tag="ACHJ7J_000452"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4998732.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADQIPVFVDT
                     VSEFKAGKIKPWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(54839..56089)
                     /gene="nupX"
                     /locus_tag="ACHJ7J_000453"
     CDS             complement(54839..56089)
                     /gene="nupX"
                     /locus_tag="ACHJ7J_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4998733.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(56189..57130)
                     /gene="rihB"
                     /locus_tag="ACHJ7J_000454"
     CDS             complement(56189..57130)
                     /gene="rihB"
                     /locus_tag="ACHJ7J_000454"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO4998734.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            57260..57958
                     /gene="yeiL"
                     /locus_tag="ACHJ7J_000455"
     CDS             57260..57958
                     /gene="yeiL"
                     /locus_tag="ACHJ7J_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO4998735.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(58026..59276)
                     /locus_tag="ACHJ7J_000456"
     CDS             complement(58026..59276)
                     /locus_tag="ACHJ7J_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4998736.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(59370..60308)
                     /gene="psuG"
                     /locus_tag="ACHJ7J_000457"
     CDS             complement(59370..60308)
                     /gene="psuG"
                     /locus_tag="ACHJ7J_000457"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO4998737.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(60296..61237)
                     /gene="psuK"
                     /locus_tag="ACHJ7J_000458"
     CDS             complement(60296..61237)
                     /gene="psuK"
                     /locus_tag="ACHJ7J_000458"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO4998738.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGYSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(61661..63352)
                     /gene="fruA"
                     /locus_tag="ACHJ7J_000459"
     CDS             complement(61661..63352)
                     /gene="fruA"
                     /locus_tag="ACHJ7J_000459"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO4998739.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(63369..64307)
                     /gene="fruK"
                     /locus_tag="ACHJ7J_000460"
     CDS             complement(63369..64307)
                     /gene="fruK"
                     /locus_tag="ACHJ7J_000460"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO4998740.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(64307..65437)
                     /gene="fruB"
                     /locus_tag="ACHJ7J_000461"
     CDS             complement(64307..65437)
                     /gene="fruB"
                     /locus_tag="ACHJ7J_000461"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO4998741.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            65805..66986
                     /gene="setB"
                     /locus_tag="ACHJ7J_000462"
     CDS             65805..66986
                     /gene="setB"
                     /locus_tag="ACHJ7J_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO4998742.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     VVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(66983..67237)
                     /gene="yeiW"
                     /locus_tag="ACHJ7J_000463"
     CDS             complement(66983..67237)
                     /gene="yeiW"
                     /locus_tag="ACHJ7J_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4998743.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            67392..67964
                     /gene="yeiP"
                     /locus_tag="ACHJ7J_000464"
     CDS             67392..67964
                     /gene="yeiP"
                     /locus_tag="ACHJ7J_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO4998744.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            68187..69653
                     /gene="yeiQ"
                     /locus_tag="ACHJ7J_000465"
     CDS             68187..69653
                     /gene="yeiQ"
                     /locus_tag="ACHJ7J_000465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4998745.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATAESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            69771..70757
                     /gene="yeiR"
                     /locus_tag="ACHJ7J_000466"
     CDS             69771..70757
                     /gene="yeiR"
                     /locus_tag="ACHJ7J_000466"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO4998746.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            70796..71509
                     /gene="lpxT"
                     /locus_tag="ACHJ7J_000467"
     CDS             70796..71509
                     /gene="lpxT"
                     /locus_tag="ACHJ7J_000467"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO4998747.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGILMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            71921..72487
                     /gene="mepS"
                     /locus_tag="ACHJ7J_000468"
     CDS             71921..72487
                     /gene="mepS"
                     /locus_tag="ACHJ7J_000468"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO4998748.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            72668..74224
                     /gene="pdeN"
                     /locus_tag="ACHJ7J_000469"
     CDS             72668..74224
                     /gene="pdeN"
                     /locus_tag="ACHJ7J_000469"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4998749.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            74306..76120
                     /gene="yejA"
                     /locus_tag="ACHJ7J_000470"
     CDS             74306..76120
                     /gene="yejA"
                     /locus_tag="ACHJ7J_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO4998750.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            76121..77215
                     /gene="yejB"
                     /locus_tag="ACHJ7J_000471"
     CDS             76121..77215
                     /gene="yejB"
                     /locus_tag="ACHJ7J_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO4998751.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            77215..78240
                     /gene="yejE"
                     /locus_tag="ACHJ7J_000472"
     CDS             77215..78240
                     /gene="yejE"
                     /locus_tag="ACHJ7J_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4998752.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLGDKGWVLWAPIRFGA
                     TSINFSTDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSIMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            78242..79831
                     /gene="yejF"
                     /locus_tag="ACHJ7J_000473"
     CDS             78242..79831
                     /gene="yejF"
                     /locus_tag="ACHJ7J_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO4998753.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(79835..80179)
                     /gene="yejG"
                     /locus_tag="ACHJ7J_000474"
     CDS             complement(79835..80179)
                     /gene="yejG"
                     /locus_tag="ACHJ7J_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO4998754.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(80512..81702)
                     /gene="bcr"
                     /locus_tag="ACHJ7J_000475"
     CDS             complement(80512..81702)
                     /gene="bcr"
                     /locus_tag="ACHJ7J_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO4998755.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(81730..82425)
                     /gene="rsuA"
                     /locus_tag="ACHJ7J_000476"
     CDS             complement(81730..82425)
                     /gene="rsuA"
                     /locus_tag="ACHJ7J_000476"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO4998756.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            82574..84334
                     /gene="radD"
                     /locus_tag="ACHJ7J_000477"
     CDS             82574..84334
                     /gene="radD"
                     /locus_tag="ACHJ7J_000477"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4998757.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRHHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMDFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            84459..84743
                     /gene="rplY"
                     /locus_tag="ACHJ7J_000478"
     CDS             84459..84743
                     /gene="rplY"
                     /locus_tag="ACHJ7J_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO4998758.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(84882..85889)
                     /gene="yejK"
                     /locus_tag="ACHJ7J_000479"
     CDS             complement(84882..85889)
                     /gene="yejK"
                     /locus_tag="ACHJ7J_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO4998759.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFMEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            86071..86298
                     /gene="yejL"
                     /locus_tag="ACHJ7J_000480"
     CDS             86071..86298
                     /gene="yejL"
                     /locus_tag="ACHJ7J_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO4998760.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            86318..88078
                     /gene="yejM"
                     /locus_tag="ACHJ7J_000481"
     CDS             86318..88078
                     /gene="yejM"
                     /locus_tag="ACHJ7J_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO4998761.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFAAYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWKKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            88153..88229
                     /locus_tag="ACHJ7J_000482"
     tRNA            88153..88229
                     /locus_tag="ACHJ7J_000482"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88187..88189,aa:Pro,seq:ggg)
     gene            complement(88331..90922)
                     /gene="yejO"
                     /locus_tag="ACHJ7J_000483"
     CDS             complement(88331..90922)
                     /gene="yejO"
                     /locus_tag="ACHJ7J_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO4998762.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKATTRVMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            91242..91889
                     /gene="narP"
                     /locus_tag="ACHJ7J_000484"
     CDS             91242..91889
                     /gene="narP"
                     /locus_tag="ACHJ7J_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO4998763.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(92100..93152)
                     /gene="ccmH"
                     /locus_tag="ACHJ7J_000485"
     CDS             complement(92100..93152)
                     /gene="ccmH"
                     /locus_tag="ACHJ7J_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO4998764.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVLEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSLQESK"
     gene            complement(93149..93706)
                     /gene="dsbE"
                     /locus_tag="ACHJ7J_000486"
     CDS             complement(93149..93706)
                     /gene="dsbE"
                     /locus_tag="ACHJ7J_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO4998765.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(93703..95646)
                     /gene="ccmF"
                     /locus_tag="ACHJ7J_000487"
     CDS             complement(93703..95646)
                     /gene="ccmF"
                     /locus_tag="ACHJ7J_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO4998766.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISMLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAVLRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVRPQKTAPEAV"
     gene            complement(95643..96122)
                     /gene="ccmE"
                     /locus_tag="ACHJ7J_000488"
     CDS             complement(95643..96122)
                     /gene="ccmE"
                     /locus_tag="ACHJ7J_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO4998767.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(96119..96328)
                     /gene="ccmD"
                     /locus_tag="ACHJ7J_000489"
     CDS             complement(96119..96328)
                     /gene="ccmD"
                     /locus_tag="ACHJ7J_000489"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO4998768.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRASQQQEAA"
     gene            complement(96325..97062)
                     /gene="ccmC"
                     /locus_tag="ACHJ7J_000490"
     CDS             complement(96325..97062)
                     /gene="ccmC"
                     /locus_tag="ACHJ7J_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO4998769.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(97104..97766)
                     /gene="ccmB"
                     /locus_tag="ACHJ7J_000491"
     CDS             complement(97104..97766)
                     /gene="ccmB"
                     /locus_tag="ACHJ7J_000491"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO4998770.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(97763..98386)
                     /gene="ccmA"
                     /locus_tag="ACHJ7J_000492"
     CDS             complement(97763..98386)
                     /gene="ccmA"
                     /locus_tag="ACHJ7J_000492"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO4998771.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(98399..99001)
                     /gene="napC"
                     /locus_tag="ACHJ7J_000493"
     CDS             complement(98399..99001)
                     /gene="napC"
                     /locus_tag="ACHJ7J_000493"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO4998772.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(99011..99460)
                     /gene="napB"
                     /locus_tag="ACHJ7J_000494"
     CDS             complement(99011..99460)
                     /gene="napB"
                     /locus_tag="ACHJ7J_000494"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO4998773.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(99457..100320)
                     /gene="napH"
                     /locus_tag="ACHJ7J_000495"
     CDS             complement(99457..100320)
                     /gene="napH"
                     /locus_tag="ACHJ7J_000495"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO4998774.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(100307..101002)
                     /gene="napG"
                     /locus_tag="ACHJ7J_000496"
     CDS             complement(100307..101002)
                     /gene="napG"
                     /locus_tag="ACHJ7J_000496"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO4998775.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(101009..103495)
                     /gene="napA"
                     /locus_tag="ACHJ7J_000497"
     CDS             complement(101009..103495)
                     /gene="napA"
                     /locus_tag="ACHJ7J_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO4998776.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(103492..103755)
                     /gene="napD"
                     /locus_tag="ACHJ7J_000498"
     CDS             complement(103492..103755)
                     /gene="napD"
                     /locus_tag="ACHJ7J_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO4998777.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(103745..104239)
                     /gene="napF"
                     /locus_tag="ACHJ7J_000499"
     CDS             complement(103745..104239)
                     /gene="napF"
                     /locus_tag="ACHJ7J_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO4998778.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            104348..>104426
                     /gene="yojO"
                     /locus_tag="ACHJ7J_000500"
     CDS             104348..>104426
                     /gene="yojO"
                     /locus_tag="ACHJ7J_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO4998779.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFA"
BASE COUNT        25327 a        28207 c        26047 g        24845 t
ORIGIN      
        1 ccagtacagc aatgagcacg gttaactttt gtgtcagcgt gtccatccgc tctccttatt
       61 aaggagcgca acaccagcac cttaccctta aactgcctgt cgatactggt ttacgtatgt
      121 tggaccacga tgttgcactc cggatcaggg caccgcctgc agcaccaccc gtccggtagc
      181 caggacttga aataggttcg atccggcgca acgtcccaac ggtctggatt ataaatctca
      241 ttatcttaat tgtaacgggg tccagccgtg gttataattc gttcgttgga acacgatgtt
      301 gcacaggctg tggtgttagg cctgaaaata gtaaataaaa gaacctgcct caccagcagg
      361 tttttttatt tactgtgatc tgctttccag atatttttcg ctcaaacaac taatgcgcca
      421 aacatttatt gcgcgtaaaa tatcgtttat ttcattaata catttcaggg atgaatatat
      481 gtcaccgcag aataatcatc tgcagcgtcc gcctgctgct gtgttatacg ccgatgaact
      541 ggcaaaatta aaacaaaatg atgccgcacc ttgcccgccc ggttggcagt taagtttgcc
      601 tgcggcccgt gcttttatcc ttggcgatag cgcgcaaaat attagccgta aagtggtgat
      661 tagtccctcc gctgtcgaac gtatgttagt cactcttgcc accgggcgcg gtttgatgtt
      721 ggtgggggaa cctggcaccg caaaatctct cctgtctgaa ttactggcaa ccgctattag
      781 cggcgatgcc gggttaacca tccagggcgg cgcatctact actgaagatc aaatcaagta
      841 cggctggaac tacgccctgc ttatcaatca tggtccatca acggaagcgc tggtccctgc
      901 cccgctttac cagggaatgc gcgacggcaa aatcgtccgt tttgaagaga taacgcgtac
      961 accgctggaa gtgcaagact gtctgttagg tatgctttct gacagggtga tgacggtgcc
     1021 ggaactcact ggtgaagcca gtcagctcta tgcgcgagaa ggctttaata ttattgccac
     1081 tgccaatacc cgcgatcgcg gcgtcaatga aatgagtgct gcgctaaagc gtcgcttcga
     1141 ttttgaaact gtctttccga ttatggattt cgcccaggag ctggagcttg tcgccagtgc
     1201 ttcggcgcgt ttgctggccc atagcggtat tccgcataaa gtgcctgacg cggtactgga
     1261 gttactggtc cgcaccttcc gcgatctgcg tgccaacggc gaaaagaaaa cctcaatgga
     1321 tacactaaca gcgattatgt ccaccgctga agccgtgaac gtggcccacg ccgtgggcgt
     1381 ccgcgcctgg tttttagcga atcgcgcagg tgagccagcc gatctggtgg actgcattgc
     1441 cggaaccatc gtcaaagata acgaagaaga tcgcgctcgt ctgcgccgtt acttcgaaca
     1501 gcgtgtcgcc acacataaag aagctcactg gcaggcttat tatcaagccc gccaccgcct
     1561 gccgtgagga aagatgcatg agcgagccgt taattgtcgg catccggcat catagtccgg
     1621 cctgcgcccg gctggtgaaa tcgttaatcg aaagccagcg gccacgatac gtgttgattg
     1681 aaggcccggc tgattttaat gaccgggtag acgaactgtt tttagcccac cagcttccgg
     1741 tagctattta cagttattgc cagtatcagg acggtgcagc ccccgggcgt ggtgcctgga
     1801 cgccatttgc tgaattttcg ccggagtggc aggcgctaca agccgctcgt cgtattcagg
     1861 cacaaactta cttcatcgat ttgccttgct gggcacagag tgaagaagag gacgattcgc
     1921 ctgatacgca agaggaaagc cagaccttgc tgctgcgtgc cacccgcatg gataacagcg
     1981 ataccctgtg ggatcacttg ttcgaagatg aaagccagca aactgcatta ccctctgcac
     2041 tggcgcacta ttttgcccaa ctgcggggcg atttccccgg cgatgcactc aatcgtcagc
     2101 gcgaagcctt tatggctcgc tggattggat gggcggtgca gcaaaataat ggcgacgtgt
     2161 tagtcgtctg cggtggctgg cacgctccgg cactggcaaa aatgtggcgc gaatgcccgc
     2221 aggacattaa caagccagaa ttgccctcgc tggcagatgc cgttacaggt tgttatctca
     2281 caccctacag tgaaaagcgc cttgatgtgc tggcaggata cctttcagga atgcctgccc
     2341 cggtctggca aagctggtgc tggcagtggg gcttacagca ggcgggagaa caactgctaa
     2401 aaacggttct tacccgtttg cgccaacacc acttgcctgc ttcgaccgcg gatatggctg
     2461 ccgctcatct gcatgcgatg gcgctggcac agttgcgcgg acataaacta ccgttacgca
     2521 ctgactggct ggatgccata gcaggctcgc tgattaaaga agccctgaac gcgccgttgc
     2581 cgtggagcta tcgcggagtt attcatcccg acaccgatcc gattctgcta acgttgatag
     2641 acacattagc gggtgacgga ttcggtaaac ttgccccttc tacgccacaa ccgcctctgc
     2701 caaaagatgt cacctgcgaa ctggaacgta ccgcaatctc tcttccggcg gagcttacct
     2761 taaatcgctt taaccccaat ggactagcgc aaagtcaggt gttacatcgt ctggcaatac
     2821 tggagatccc agggattgta cgccagcagg gaagcacact gacacttgca ggcaacggtg
     2881 aagaacgctg gaaattaacc cgcccgctta gccagcatgc ggcattgatt gaggccgcct
     2941 gctttggtgc cacactccag gaagccgcac gccataaatt agaagccgat atgctggacg
     3001 cgggtggaat cggcagtatc accacatgtc ttagccaggc ggcgttagcg ggtctggcgt
     3061 ccttcagtca acaattactg gagcaactca cattattaat cgcccaggaa aatcaatttg
     3121 ccgaaatggg ccaggcgctg gaagtgctat atgccttatg gcggctggat gaaattagcg
     3181 gtatgcaagg cgcgcagata ttacaaacga cgttatgcgc ggctatcgat cgcacgctgt
     3241 ggctgtgtga atccaacggc agaccggatg aaaaggagtt tcacgctcac ctgcatagct
     3301 ggcaagcgtt gtgtcatatc ctgcgtgatc tgcatagcgg tgtgcagctc cccggcattt
     3361 ctctttctgc ggcggtagcc ttactggagc gacgcagtca ggcaattcat gccccggcgc
     3421 tggatcgcgg cgcggctctt ggcgcactga tgcgtctgga acatcccaac gccagtgccg
     3481 aagcggcgct gacgatgctg gcgcaattat ccccggcaca atccggcgag gcgctgcacg
     3541 gtttgctggc attagcccgt catcaactgg cctgtcaacc tgcatttatc gccggtttca
     3601 gcagccattt gaatcaacta agtgacgacg attttattaa cgccctgccc gatttacgcg
     3661 cggcgatggc ctggctacca ccacgagaac gcgggacgct ggcgcatcag gtgcttgagc
     3721 attatcaact ggcgcaactt cccgtttcgg cgctgcaaat gccgttgcat tgtccaccac
     3781 aagccattgc acatcatcaa caactcgaac agcaggcact ggcatcgctg caaaactggg
     3841 gagttttcca tgtctgaact gaacgatctt ctgaccaccc gtgagctaca acgctggcga
     3901 ttaattcttg gcgaagcggc agaaacgacg ctttgtgggc tggatgacaa cgcccggcag
     3961 atagaccacg cgctggagtg gctgtatggg cgcgatcctg aacggctcca gcgtggtgaa
     4021 cgttccggtg gattaggtag ctcaaatctc accacccctg agtggatcaa cagtattcac
     4081 acgctgtttc cgcaacaggt gattgagcgg ctggaaagcg atgccgtact gcgctacggc
     4141 attgaagatg tggtgacgaa tctcgacgtg ctggaacgta tgcagccttc tgaaagtctg
     4201 ctacgcgccg ttttgcacac caaacatctg atgaaccccg aagtactggc tgccgcccgc
     4261 cggatagtgc gccaggttgt tgaagaaatt atggctcgac tggcaaagga agttcgtcag
     4321 gctttttctg gtgtccgcga tcgccgtcgc cgctcattta ttccactggc gcgaaacttt
     4381 gatttcaaaa gtactcttcg cgccaacctg caacactggc acccgcaaca cggcaagttg
     4441 tatatcgaat ccccccgctt taacagccgc attaaacgcc aaagcgaaca atggcaactg
     4501 gtcttactgg ttgatcaaag cggatcgatg gtggactcgg tgatccactc tgcggtgatg
     4561 gcggcctgtt tgtggcagtt acccggcatt cgtacccatc tggtggcgtt tgacacaagc
     4621 gtcgttgatc tcacggcaga cgttgccgat ccggtagagt tattaatgaa agtgcaatta
     4681 ggcggcggaa ccaatatcgc cagtgccgtg gagtatggtc ggcaacttat tgaacaacca
     4741 gcaaaaagcg tcattatcct cgtgagcgat ttttatgaag gaggttcatc atcattgctg
     4801 acgcatcagg tgaaaaagtg tgtccagagc ggcatcaaag tgctgggact ggcagcgctc
     4861 gatagcacag caacgccttg ctatgaccgc gatacggccc aggcgctggt taatgtcggc
     4921 gcacaaatag ccgccatgac accgggcgaa ctggcatcat ggcttgcgga gaatcttcag
     4981 tcatgaattc actacgtccg gaattattag aactgacacc gcaggcactg acggcgttaa
     5041 gcaatgccgg ttttgttaag cgcagcctta aggaactgga aaatggcaac gtcccggaaa
     5101 tcagccatga gaacggcgct ttaatcgcca ccttcagtga cggtgtccgt acccagctgg
     5161 cgaacggtca ggcactgaaa gaggctcagt gcacctgcgg ggctagcggc atgtgccgtc
     5221 atcgcgtgat gctggtgtta agttatcagc gactttgtgc caccactcag tctacggaaa
     5281 aagaagaaga gtgggatccg gcaatctggc tggaagaact ggctaccctt cccgatgcca
     5341 cccgcaaacg cgcacaggcg ctggtcgcta aaggcatcac cattgagttg ttctgtacac
     5401 cgggcgaaat tccctctgcc cgcttaccga tgagcgatgt gcgtttttat tcccgcagca
     5461 gtattcgttt cgcccgttgt gattgtattg aaggcacact ttgcgaacat gtcgtactgg
     5521 ctgtgcaggc cttcgtcgag gccaaaactc agcaagcgga atttactcat ttaatctggc
     5581 agatgcgcag cgaacacgtc acatcatctg acgatccgtt tgccagcgaa gaaggccaaa
     5641 cgtgtcgtca atatattcag caattaagtc aggcattatg gctgggcggc atcagccagc
     5701 cgccaatcca ttacgaggcc gctttcagcc gcgcacagca ggcggcggaa cgctgcaact
     5761 ggcgatgggt gagtgaatcg ctacggcaac tgcgcgccag cgttgatgcc ttccacgccc
     5821 gcgccagcca ctatcatgcc ggagaatgct tacgtcagct tgcggcatta aacagtcgat
     5881 taaattgcgc acaagagatg gcccggcgcg acagtgttgg tgaagttcct cctgtgccgt
     5941 ggcgcacggt cgttggctct ggcattgccg gagaagcaaa gcttgatcat ctgcggctgg
     6001 tgtctttagg tatgcgttgc tggcaggata ttgagcatta tggtttacgc atctggttta
     6061 ccgatcccga caccggcagt attttgcacc tttcgcgcag ttggccgcga agtgaacagg
     6121 aaaactcacc ggcagctacg cgtcggctgt ttagttttca ggctggcgca ctggcgggcg
     6181 ggcaaattgt ttcacaagca gcaaaacgca gtgccgatgg cgagctgctg ttagctaccc
     6241 gcaaccgctt aagcagcgtt gtgccgctgt cgcctgatgc ctggcaaatg ttgagcgcgc
     6301 cgttacgcca gccgggcatt gtggctttgc gggaatattt acaccagcgt ccccccgcct
     6361 gcatacggcc tcttaatcag gtcgataact tatttattct gccggtcgct gagtgtattt
     6421 cgctcggttg ggatagcagc cgccagacgt tggatgcgca ggtaatcagc ggcgaaggtg
     6481 aagataatca gctgacgtta tcactgccag cctcagccag cgcgccttat gctgttgaac
     6541 gcatggcggc gcttttgcaa caaacagacg acccgtgtgt ctggtttctg gctttgtcag
     6601 ttttgttgat gggcaattga cactggaacc acaggtaatg atgacaaaga ctcgtgcctg
     6661 ggcgctggac gcagaaactg cgcctgtggt ggcatcacta ccttctgcca gcgttctgcc
     6721 tgtgccgtct actgctcatc agttgctgat gcgctgccag gcattactta ttcaactgct
     6781 ccataacggc tggcgctatc aggaacagag tgctattggt caggcagagt tgctggcgaa
     6841 tgacctcacc gcggtgggtt tttatcggct ggcacatgta ttgggacaat ttcgtaatac
     6901 agaaagcgag gcacgggtag aagcaatgaa taacggtgtt ttgctgtgcg aacaattatt
     6961 ccccatgctt cagcaacaag gatgaaatag tgctttttac taagagttct actccagttc
     7021 cggactgctc acgccacggt attaggcata tcctatatag cccctggtga gagtcaccag
     7081 ttccttgatt aaataaaatg gagttttaca tgaaggcttt caataagctg ttttcccttg
     7141 ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg tgacaaagaa gaatcgaaga
     7201 aattcagcgc caatctgaac ggcactgaaa ttgccattac ctatgtctac aaaggtgaca
     7261 aggtacttaa gcaatcttct gaaaccaaaa ttcaatttgc ctccattggt gcaaccacca
     7321 aagaagacgc tgccaggaca cttgagccgt taagcgccaa atacaaaaac atcgcgggtg
     7381 ttgaagaaaa attaacctat accgatacct acgcgcagga aaacgtgact atcgatatgg
     7441 aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat caacgtttct gctgaagatg
     7501 ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat gaaagccgct ggttttaaag
     7561 aagtgaaata atctgtcggc ggccatgttt cgcatggccg ccatacccgt cttagctttt
     7621 cttcacatgc tggcgcgctg ccagtccacg cagaaaataa cgtaaaaatt gatcgccgca
     7681 ttcgcggaag tttttatgat ccggtgcgcg catcatcgct gtaatttccg gcatcgaaac
     7741 gcggaactgc tgttcggtga ggatcgccag aatgtcatcg gttttcagcg aaaacgcgat
     7801 gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt tccggctcca gtgccggagc
     7861 agactcatcc ttgccgcgtt tttcataaat caggccattg aggaatgacg acaaaacaat
     7921 gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt agccagacgg cgatctgttc
     7981 cgcggtggct tcgacattac ccagcgccag tatacgcacc aggtcattat tattggcttt
     8041 caaaatgtag cgcacgctgc gcagaatatc gttacttagc atgaggcctt caggtgttga
     8101 tgaggcaaaa agccatttta gcagtctttt acaggccaat cgcctctttt aagcttttca
     8161 gataacggcg gctgaccggc acggttaagc cattacgcaa aatcaactcg gcctggccgt
     8221 tatcttccag acgaatctcc tgtaaatgcg cgaggttaac cagatactga cgatggcagc
     8281 gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa ttcggtaaag ccctctttcc
     8341 cttcgtggct ggtaacgtag acaccgctca tccgactgct gacaaatgcc acatctttca
     8401 tttgcagcaa ataaatccga ctatgcccag tacaagggat aaatttcagc gcctgttgat
     8461 tttctggtaa cagcgaaaca tcctgcttgc tgcgttcctg ccgcaaacgc gccagcgttt
     8521 tctccagtcg cgcttcatca attggcttca gcagataatc aaaggcgtgt tcttcaaagg
     8581 ctttaatggc gtattcgtca aacgcggtga gaaaaacaat atacgggcga tgttccgggt
     8641 caagcatccc caccatttcc agaccactga tgcgcggcat ctggatatcg agaaacagca
     8701 catccgggcg cagtttatgc accgcgccga tcccttccac ggcgtttgaa cactcaccaa
     8761 cgatttcaat atcgctctgc tcctgcaaaa atacgcgcag gttctcccgt gctaacggtt
     8821 catcatcgac aattaagact ttaatcatgc ctcgtccctc catggtagtc gtaacgttat
     8881 tcgggtgtaa ctatcaggct cacaggcgac gcttatccca tagtcatcgc caaaccgttc
     8941 acgtaaacgc ttatccacca gattcatccc cagcccactg gcattggtta ccggttgata
     9001 caaaccggca ttgtcttcga tctccagcat taaatgttgc ccctcacgtc gggcgctgat
     9061 tgccactcgc cctgtatcca gcagttgtga tgtcccatgt ttaatggcgt tttccactat
     9121 cggttgcagg gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa tagcaatgtt
     9181 gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc agataagcat tcacatgttc
     9241 aatttcgtcg gcgagagtaa caaactccga aggccgcttt aagtttttgc ggaaaaaagt
     9301 ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg cggcggatca ccgctttaat
     9361 ggtgttaagc gcattaaaca aaaaatgggg gttcacctgg gcgtgaagca gtttgatctc
     9421 tgactgggtg agcatcgctt tttgccgctc atattgcccg gcaaggatct gcgccgaaag
     9481 cagttgcgca atcccctcac ccaacgtgcg gttgattgaa ctgaataaac ggtttttggc
     9541 ttcatacaat ttgatggtgc ccatcacccg ctgattttca ccacgtaacg gaattaccag
     9601 cgtcgacccc agtttgcatt gcggatgcaa agagcaacgg taaggtactt cgttgccatc
     9661 agcgtagacc acttcaccgg tttcaatcgc ttttaaggta taagtcgaag aaatcggttt
     9721 gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc agcaatttct ctcgatcggt
     9781 aatcgcgact gcaccaatat ccagctcctg atacagcacc tgtgccactt tcatgctgtt
     9841 cacttcgtta aacccctggc gcaaaatgcc ttccgtcgag gccgccactt tcagcgcagt
     9901 ggcagaaaaa gctgaagtgt atttttcaaa catcgcgcgt ttatcgagca atatacgcat
     9961 aaacagcgcc gcgccgacgg tattggtgac catcattggc gcagcaatat tactcaccag
    10021 acgcaccgca tcttcataag gtcgggcgat cgccaggatg atcaacattt gcaccatttc
    10081 agcgacgaac gtgacggcac cggcggtaat ggggttaaag actttatcag tgcgcccgcg
    10141 acggatcagg atgctgtgta ccaggccgcc gagcaatcct tcaacgatgg tcgagatcat
    10201 gcaacttagc gcggtcatgc cccccatcga atatcgatgt aacccgccgg tcaggccaac
    10261 cagcccaccg acgaccggac cgccgagtaa gccgcccatt accgcgccta tcgcacgggt
    10321 attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc agaagatgga
    10381 aaagacgatg tagcagagaa atttatgcgg cagacgaacc gtgacctgca ttaacggtat
    10441 gaataatggc gttttactca ttaaccacgc aatgactaaa aaaacgcaca tctgctgaag
    10501 cagcagcaac accagattaa aatcgtacat acccgcaaac cacacttccc tttaaaacgc
    10561 gtaacataca ttgcctgcgt ttaactttct ttgaactctt gcagaaaaat gagaattcgt
    10621 gagtacgatc actcaaaatc gcctggcaaa aataaaatca ccctataaat gcacaaaaaa
    10681 cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt aaaccgggac ctcgcgagca
    10741 agggtgagac gatggcgctt tacacaattg gtgaagtggc gttgctttgt gatattaacc
    10801 ctgtcacgtt acgcgcgtgg cagaggcgtt acggattgct gaaaccgcaa cggacagacg
    10861 gcggtcatcg gctgttcaac gatgccgata ttgaccggat ccgcgagatc aaacgctgga
    10921 tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct cagtaatgaa aatgttgatg
    10981 tgcagaacgg ctggcgcgat cagcaagaaa cattactgac ttacttgcaa agcggcaatc
    11041 tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga ttaccccgcc cagacgctca
    11101 ccacgcatct gtttattcct ctgcgccgac ggcttcagtg ccaacaaccg actctccagg
    11161 cgctgctggc gatcctcgac ggcgtactga tcaactacat cgccatctgt ctggcttcgg
    11221 cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg gaatattcac gataccaccc
    11281 gtctgtggct ggagggctgg attgccagtc aacaaggatg gcgcattgat gtcctcgccc
    11341 actcgctcaa tcaactacgc cctgaactgt tcgaaggccg tacattgctg gtgtggtgcg
    11401 gtgaaaatcg aacctccgcc caacagcagc aactcaccag ttggcaagaa caaggccatg
    11461 atattttccc actcggcatt taatgattcg ttaacaaatg cgctttactg tacaatcctt
    11521 tcgttaacat aaggagtgca ttatgcgcat agctaaaatt ggggtcatcg ccctgttcct
    11581 gtttatggcg ttaggcggaa ttggtggcgt catgctcgca ggttatacct ttattttgcg
    11641 tgctggctaa gcgcctgcac cagcctttca aacaggcggt ctgcgatgat cgccgccagt
    11701 gccaccagta acgccccctg gatcacatac gcggtattaa atccgctaag cccgatgatg
    11761 atgggcgtac ccagcgtgct ggcccctacc gttgaggcga tcgtcgccgt accaatgttg
    11821 ataatcaccg aagttcgcac gcccgccaga atcaccggag ccgccagcgg tagctcgacc
    11881 ttacgcagtc gctgacctcg actcattccc atacctttcg caacttctgt cacgctggca
    11941 tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg gcagcacacc ataaaggatc
    12001 aaggcgataa tcgctggttg cagaccaaac ccgatcaccg gaacggcgat cgccagcact
    12061 gcgacgggcg gaaaagtctg cccaacggcg gcaatagttt ccaccagtgg gcgaaattcc
    12121 gcgccccacg ggcgagtgac tgcaattccg gcaccagtgc caatgatcac cgcaaacaaa
    12181 ctcgaaattc ccaccagcca gaaatgagcc agtgccagag ctgcaaaact ttcttgctga
    12241 taaacgggtc gtggcagttg tgggaacaag gcagcaaaca gcggctggct gtaaggcagc
    12301 cagaaaatca gtgccacaaa caaagcaatg agccagaaca gcggatcgcg caacatcttc
    12361 atacgattac gcctcctcca gcagatcctg aaaatgcagc gtgccgcaag gctggccctg
    12421 cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca aacagggaga gcgcatcgcg
    12481 tagcgtcatc tcttctgcca gtgcctcacc ttctgctcgt tcttcgcgac gcacgtaatc
    12541 cgccacacta cgtaacgaaa gcaggcgcac acccagttca ctacgtccaa aaaactggcg
    12601 gacaaaatca ttcgccggac gagtcagcat cgtcagcgga ttcccctgct gcaccacttc
    12661 accgtgatcc atcaatacca gatgttctgc cagccgtagc gcctcatcaa tatcatgagt
    12721 gaccagcaca atagtgcgcc ccagcaaacg gtgaatgcgc gtcatctctt gttgcaacgc
    12781 gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc atcagtaaga cttgcggatc
    12841 ggcagccagt gcgcgcgcca ctcccacacg ttgctgctga ccaccggaaa gctgatgcgg
    12901 ataacgctca cgcaaatttg actccagccc cagtagcgcc attaattcgt cgatacgatc
    12961 gtcaatcctc gcccgcgacc atttttgtaa ttgcggcacg gtggcaatgt tttgtgccac
    13021 gctccagtgg ggaaacaggc caatagattg aatggcatag cccatacggc ggcgcaactc
    13081 cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg atctctccgc tgtcatgttc
    13141 caccaggcgg ttaatcattt tcagggtggt ggatttgccg gagccagatg tgccaatcag
    13201 caccgaaaaa ctcccttcct gaaaattgag attgagatcg ttaacggctt tttgtgcgcc
    13261 aaacagtttg ctgacatggc taaattcaat cattacgttt caccttcaac agtgcgataa
    13321 gtaaatcgaa cagcgcatcg atcagcaccg ccagaacaat taccgggatc acccccagca
    13381 acactaaatc aatggcgctg cttagcagcc cctggaaaac cagcgcacca aaaccgcctg
    13441 cgccgattaa cgccgcaatc accgccatgc ctacagtttg caccatcacc acccgcaggc
    13501 tgcgcagaaa taccggtaac gccagcggta actgaacatg caggaatcgc tgcgccccgc
    13561 tcatccccat cgctctggcg ctctccagca catcgcgcgg gatctggttc aagcctacta
    13621 ccacgccgcg caccagcggc agcaaggcat agagcaccag cgcaatcagt gcgggtgtca
    13681 ttccggttcc tgctatgccg agcttcccca gccacggaaa ggccgtcacc agcgcggcaa
    13741 gcggcgcaat caacaggcca aagagcgcca ccgaaggcac ggtctgaatg acattgagca
    13801 gagaaaaaat cgccccctgc cgcgcggtgg aaaagtagca ccagatgccc aatggcacac
    13861 caatcactaa cgcaggcagc accgcaccaa acagcaacgt cagatgttga gccagcgcgt
    13921 cgtcaaacac atcctgacgg ttggcgtatt ctttcattag cgagagatcg ttaagcgtgc
    13981 cggagtaaag caaccacagc ggaataatgg caatctgcat atgcaacaac cagcgccaca
    14041 gcggatgcgt ggagattcgg cggatggcat cgctacaggc cagcaatgcc agcgccgcag
    14101 ccagccagaa accactgccg aggctggtac gcgccagcgc actgccattt tgcgccagtt
    14161 gggtcgccgc ctttccagct ccccacacca gcaatacgaa gacgaattgc gccagaatga
    14221 gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc tgtcagccag gcgcaaccaa
    14281 cgcccaccag catccagatc gtttgcggcc acagttgcca gagatgacgc ccttcacccg
    14341 agactaaacg attaggcgcg tagctgataa acggcagcgc tgctgcgatt gccgtcagca
    14401 acagcagcag cgccagaaca ggattaatac gtagataagt cacgggaaat tacttcgtcc
    14461 accctttttg tttcaggtag tcggcagcca cttttttggc atccagtcct tccactgcaa
    14521 tgctggcatt cagttgctgc aatgtttttg catcgaggct ggcgaagact ggctgtagcc
    14581 actgtgccat ttgcggatac tctttcagca ccgactcacg caccactggt gcaggcgcgt
    14641 agataggttg cacgccttgc ggatcgctta aggtttgcag ccccagcgcc gcgaccggac
    14701 cgtcagtgcc gtaagccatt gcggcattaa cgccagaagt ttgctgggca gcggctttaa
    14761 tcgtcaccgc cgtgtcgcca ccagccagtg acagcaactg atcctgaccg agcttaaagc
    14821 cataggcttt ttcaaacgcg ggtaaggcat cggcgcgttc gataaactct gccgaggctg
    14881 ccagtttgaa ggtgccgccc tctttcagat aacgactcag gtcagcaagc gaagtgagtt
    14941 tgtttttctc tgccacatcc tgacgcacgg cgatggtcca ggtgttattt gcaggtgcgg
    15001 gcgtcagcca gattaacttg ttttgctctg catcgagttt tttgactttc tcgtaacctt
    15061 gctgcgcgtt tttccatgcc gcatcgtttt catctttaaa gaagaaagcg ccattaccgg
    15121 tatattccgg atagatatcc agttcaccgg aagtaatcgc cccgcgcacc acaggagtcg
    15181 ttccaagttg cactttattg accgttggta ctccgtggct ttcgagtact tgcaaaatga
    15241 tattgccgag cagcgcgcct tcggtatcga tttttgaacc gactttaacg ggagaagctg
    15301 cctgtagcgg caggctcacg gctgccaaca taaccagtga acctgcccag acctttgaga
    15361 gtggcatgat gctttcctca ttcttttact gttgttttca gcgaattaag agaaaagcat
    15421 agttgataat gacagagtta gcctgagcgg acggattcag ttgcagaatc agataaatac
    15481 ttaagaggca tattcggtcc ggcgtttcct gccggatgcg gcgcgagcgc cttatccggc
    15541 ctacaaaggg cgaaaattca gcaagttaag atatgaacat caggcaaagg gcgcaaacgt
    15601 cgcgcccttg ctaaagcatt acagcaactc aaactcgcct ttcttaacgc gtgcggaatc
    15661 agtgccgata aagacattga acttgccagg ctcggcgtca tatttcatct gttgattcca
    15721 gaacttcagc gcctcgatat caatcgggaa gctaacggtc tgagtttcgc ccggtttcag
    15781 ggtgattttc tcaaagcctt tcagctgttt caccggacga ctcatggaag ccgtcacatc
    15841 ctgcaagtac atctgcacta ccgtggcccc ttcgcgctta ccggtgttcg tcacctgcac
    15901 gctggccgtt actttaccgt cacgcttcat ggtcggcgca gaaagtttca cgtcagagac
    15961 ggtgaaagtg gtgtagctca gaccatagcc gaacggataa agcgccccgt tagcttcatc
    16021 aaaataacgc gaagtgtatt tgttcggctt gtcggcgtta tatgggcgac cggtattcag
    16081 atggctgtag tacacaggga tttgcccgac agatcgcggg aaggacatcg gcagcttgcc
    16141 ggacgggttg taatcgccaa acaatacatc ggcaattgca ttaccgcctt cagtccccgc
    16201 aaaccaggtt tccagaatcg catcagcctg ctgatcttct ttcaccagcg ccagcggacg
    16261 cccgttcatc agcaccagca ccagcggttt accggtggct ttcagcgccg caatcaagtc
    16321 acgttggctt tgcggaatag taatatcggt acggctggag gcctcgtgcg ccatcccctg
    16381 tgcttcaccg actacagcca ccaccacatc agactgtttc gccgtctgca ccgcttcatc
    16441 aatcatctct tgcggcgagc gcggatcgac ttttaccgct tcttcatact gattcaggaa
    16501 gtcgataatg cctttgtcac tggtaatgtt cgcccctttg gcatacagca ctttaccgtt
    16561 ttcaccgacg gcatttttaa tcccggtcaa cacggtcacg gattgatcgg caacacctgc
    16621 cgccgaccag cttcccatca cgtcacgttt actgtccgcc agtggcccaa ccaccgcaat
    16681 ggtggccgat tttttcagcg gtaacgtttc gagacggttt ttcagcaaca ccaggctttc
    16741 acgcgccact tcacgcgctt ctttacggtg caggcggctt tcggcattgg tatccatcgg
    16801 gtcagactct ttcggaccga gatggctgta tgggtcgtta aacaacccca tatcatattt
    16861 aacgttcagt acatggcggg cagcatcgtc cagctctgcc atcgtcactt tgccggattt
    16921 gatcaaccca ggcagatact tcgagtagta ctcgtcgctc atgctcatgt tgattccgga
    16981 tttcagcgcc acgcgcaccg catcttccgg gtctgccgcc gtgccatgtt taatcagttc
    17041 tttgattgca ccatgatcgg aaacggtgat gcctttaaag ccccactggt cgcgcagaac
    17101 atctttcagc agccaggagt cggaggtggc tggcgtgccg ttgagtgagt tcagcgccac
    17161 cattaccgca ccgctgcctg cgtccagccc cgctttgtac ggcggcatat aatcattaaa
    17221 caggcgctgc ggactcatat cgacggtgtt gtactcttta ccgccttcta ccgcaccgta
    17281 tgcggcaaag tgtttgacgc tggtcatcac cgagtagcga tctgccgggc ttttaccctg
    17341 catcgcttcc accatggttt tacccattat tgaggtgaga tacgtatctt cgccaaaacc
    17401 ttcggaagca cgtccccagc gcggatcgcg cgagacatcg accatcggtg cccaggtcat
    17461 attcaggcca tcatctgccg cttcataagc agagacacgt ccgaccgttt tcactgcatc
    17521 gaggttaaaa gacgaggcca gaccgaggct aatcgggaac accgtgcgct gaccgtgcag
    17581 cacgtcgtaa gcaaagaaaa gaggaatttt caggcggctt aattccatca cctgatcctg
    17641 catggcgcgg atatcctgac gggttacggt gttgaaaatc gccccaacct gaccgtcttt
    17701 gatcatctcg cggatcgcct ctttcgggtt atccgggccg acgctgatta agcgcagctg
    17761 accaattttc tcatcaactg tcattttctt aagcagttcg gtgacgaacg catcccgcgc
    17821 ttcaggcgtt aatggatggt taccgaataa atcatccgcc agtgctggct gcagggccag
    17881 actcaccgcg attcctactg aacatagcca tttcatatgg atttttcttc ctttgttgcc
    17941 cgacgtggca gcgaaaatgg tgcaaaaacc gtagtttgcc ataagcatga tggagagaga
    18001 aaaagaatgc tcagtttatt gtctgaattt tcaaaatatt cactcgctga attgtcatac
    18061 aaggcgctat tctagtttgt gatatttttt cgccaccaca aggagtggaa aatgtcttcc
    18121 atgacaacaa ctgataataa agcctttttg aatgaacttg cccgtctggt cggtcattca
    18181 cacctgctca ccgatcccgc aaaaacggcc cgctatcgca agggcttccg ttctggtcag
    18241 ggcgacgcgc tggctgtcgt tttccctggc tcactactgg aattgtggcg ggtgctgaaa
    18301 gcctgcgtca ccgccgacaa aattattctg atgcaggctg ccaatacagg cctgaccgaa
    18361 ggatcgacgc caaacggtaa cgattatgat cgcgatatcg tgatcatcag caccctgcgt
    18421 ctcgacaagc tgcacgttct cggcaagggc gaacaggtgc tggcctatcc gggcaccacg
    18481 ctttattcgc tggaaaaagc cctcaaaccg ctgggacgcg aaccgcactc ggtgattgga
    18541 tcatcgtgca ttggcgcatc ggttatcggc ggtatctgta ataactctgg tggttcgctg
    18601 gtgcagcgtg gcccggcgta taccgaaatg tcgttattcg cgcgtataaa tgaagatggc
    18661 aaactgacgc tggtgaacca tctggggatt gatctgggcg aaacgccgga gcagatcctt
    18721 agcaagctgg atgacgatcg catcaaagat gacgatgtgc gtcacgatgg tcgtcacgcc
    18781 cacgattatg actatgtcca ccgcgttcgt gatattgaag ccgacacgcc cgcacgttat
    18841 aacgccgatc cggatcggtt atttgaatct tctggttgcg ccgggaagct ggccgtcttt
    18901 gcagtacgtc ttgatacctt cgaagcggaa aaaaatcagc aggtgtttta tatcggcacc
    18961 aaccagccgg aagtgctgac cgaaatccgc cgtcatattc tggctaactt cgaaaatctg
    19021 ccggttgccg gggaatatat gcaccgggat atctacgata ttgcggaaaa atacggcaaa
    19081 gacaccttcc tgatgattga taagttaggc accgacaaga tgccgttctt ctttaatctc
    19141 aagggacgca ccgatgcgat gctggagaaa gtgaaattct tccgtccgca ttttactgac
    19201 cgtgcgatgc aaaaattcgg tcacctgttc cccagccatt taccgccgcg catgaaaaac
    19261 tggcgcgata aatacgagca tcatctgctg ttaaaaatgg cgggcgatgg cgtgggtgaa
    19321 gccaaatcgt ggctggtgga ttatttcaaa caggccgagg gcgatttctt tgtctgtacg
    19381 ccggaggaag gcagcaaagc gtttttacac cgtttcgccg ctgcgggcgc agcaattcgt
    19441 tatcaggcgg tgcattccga tgaagtcgaa gacattctgg cgctggatat cgctctgcgg
    19501 cgtaacgaca ccgaatggta tgagcattta ccgccggagc tcgacagcca gctggtgcac
    19561 aagctctatt acggccattt tatgtgctat gtcttccatc aggattacat cgttaaaaaa
    19621 ggcgtggatg tgcatgcgtt gaaagaacag atgctggaac tgctgcagca gcgtggcgcg
    19681 caatatcctg ccgaacataa cgtcggtcat ttgtataaag caccggagac gttgcagaag
    19741 ttttatcgcg agaacgatcc gaccaacagt atgaatccgg ggatcggtaa aaccagtaag
    19801 cggaaaaact ggcaggaagt ggagtaaaaa ttacggatgg cagagtatcg ccatccgtaa
    19861 ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca tttgggcggc tttttgtttt
    19921 ttatagctca acgctgctgc cggaacaggc atcactttac cggtttcaat ccaggtacgc
    19981 aggcggctgg catcggcaaa atgggtatat ttgccaaacg catccatcac taccagcgcc
    20041 accggtttat tattgataac cgtacgcatc accagacaat ggcccgccgc attggtaaag
    20101 ccggttttgg ttagctggat attccagtta tcgcgataca ccagatgatt agtattgcgg
    20161 aacggcagcg tatacgtcgg gttagagaag gttgccatat cttcccgggt ggtacttaac
    20221 tgcccgatca acggatattg tttgctggca atgagcagtt tggttaagtc acgggcggtt
    20281 gaaacgttat gcaccgatag tccggtcggt tcaacaaagc gcgtgttgtt cattccgagc
    20341 gatttcgctt tcgcattcat cgccttaata aaggctttgt aaccaccggg ataatggtgc
    20401 gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg ccagcaacag catatcttta
    20461 cggctgattt cgctattcag tcgtacgcgc gaatagaccc ctttcatctc cggcgtctgg
    20521 ctgatatcca cttttagttt ttcatccagc ggcagtcgtg catccagcac gaccatcgca
    20581 gtcattaatt tgctgataga cgcaatcgga cgcaccaaat ccgggtggtt cgaatagatc
    20641 actttgttgg tattcagatc aacaatcatc gcgctaccgg aggcaatttc cggttgtgaa
    20701 gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg ccaaaggcac agccagcatc
    20761 agggccaggc taaataaaga aactcgaaat ttcggcatga tgagcattca gatagtggtt
    20821 cacgcgcacg ggttgcgcac cgccggagta aggatttact gaggctagcg acgccatcat
    20881 aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg aaatgctgca ttgctgacat
    20941 ttacgccagc aatgcaacgt caaagaactt tctagaacaa acgataaccg tagccccata
    21001 gtataacggt aagggcgagc agcacttcca gtaccagcac gccaatcgcc agcgtcgaac
    21061 tggagaagct aaggccttcc tctttgttga tattcaggaa gctcggaata ccaaggtaaa
    21121 gcaggtaacc ggtgtaaaac agtgccaccg tgccgaccag cgcacacaac cagaccagcg
    21181 gataaagcgc caccagaccg cttaaaaaca gcggagttgc aacgtagccc gcgaagacca
    21241 tgcagtgcgc aagtgacgga cgctgcgggt aattacgcgc catccaccag atgacccgcc
    21301 ccatcaccgc aaccccagcc agcataacgc cgtaaaacaa gacagccagc gccagtccgg
    21361 taaaccagga taacttcagg atagtgccat cgccaaaatt ccagccaatc tgtgtagtgc
    21421 caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa aacgtggtgg gtgtaatggt
    21481 gagaaatcgt ttcgttttcg cgattaatca cctgcatttc acgatcggga tgggaaaaca
    21541 gtccccagac atggctcata ccgccccctt gttgtgagtt catgaacctg acagttcaag
    21601 tataagtcag cttgtgatta tttttttgtt cacccatgta atttgcaccg tctgatgtac
    21661 gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca aagggagaca
    21721 gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggtgatc
    21781 ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gcgttgcggc gcatcagggg
    21841 ctattaaagt tcccgctggt ggtactttct gtggcgcttg gcggcatgat tggcgaccag
    21901 gtgctttatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt ctcgaaacat
    21961 caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct gtttgtcatt
    22021 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    22081 ctgccgccga aaatctttct gccgcttaat attctcggcg catttgcctg ggcgttgatt
    22141 tttaccacta tcggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgat
    22201 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt aggcgtgcgc
    22261 tggtggctga aacgacgcgg gaagaaaaag ccggataatc aggcgtaaaa ccattgccct
    22321 ggataaggcg ttcgcgccgc atccgacatc cggggcacca tgcctgatgc gacgcttgtg
    22381 cgtcttatca tgcctaccaa aattttaccc attgaaataa ccacgcacct cactgcgcta
    22441 ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc
    22501 ggtggtgtaa tttgcgccct ctgagcacag ccacgctacc aggctggcaa tctcatgcgt
    22561 tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctgtcatc
    22621 catgccattc attggcgtgg cgatcgcccc aggcgcgact gcgttcacca aaatcttatg
    22681 cctgaccagc tccagcgcca ttgctttggt taacccgccg agcgcatgtt tagcggctgt
    22741 gtaggcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg
    22801 accaccctgc ccttgtttca ccatctggcg ggccgcaatt tgtgagcata aaaatgcacc
    22861 atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcgc
    22921 tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccagcc gttgaatgag
    22981 tttctccaac gcctgcgcac cttctggcag tttaccgaga tccagctgca caatctccgc
    23041 acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg
    23101 ccaggtaatg ccaatatcaa acccttgctg cgccagtaat aacgcgcact ctttgccgat
    23161 ccccgaatcg gaggcggtaa taatcgctac ctgtgccatc gagttctcca cttaacgctg
    23221 aataaacgtt aagtatagaa gtcacatatc atcagcgttt gtaacccccg cccaacgcac
    23281 cagtgagttg aatggaggca tccagccact gcccttgcaa taacaggcca ttggcccgct
    23341 cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa
    23401 agcgggcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct
    23461 ggtttttctc cgccagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt
    23521 caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcga
    23581 gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca
    23641 tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg
    23701 ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg
    23761 actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca
    23821 gttcatcagg aagctggctt gccactttcg gcaacgcgac cggtttaagc ttcaatgact
    23881 ttgtctggtg atttgtaagt gcgcttaacc gtgcctcaat aattttcatt ttccccgcga
    23941 catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg
    24001 aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt
    24061 tttcgatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc
    24121 gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc agtgctttaa
    24181 ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc
    24241 ccgccgttaa gccaaaagtt ccgttggtgt accacggacc ggtcgtacct gcggccggat
    24301 cgttcagagc aaacggcccc attagccctt ctgccgacat tttttgccgc tccatatccg
    24361 cagaaaagtc gatctgcgga ccatcctgag tggcaattgc cttcgcctgg gcttcagcta
    24421 gctgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta
    24481 atgaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt
    24541 tcagcgccgt gggtaatgcg gtatcaactt gtgcagcggg cgtttgctgg cttaacgcct
    24601 ggcgggtttc atgcataggc gcacacccgg tcagcatcag taacagcgga aaacaggcga
    24661 tggctggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg
    24721 agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata
    24781 aaacgacata tccagaaaaa tatacactaa gtgaatgata tcttccgatt tatcttaatc
    24841 gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata
    24901 aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac
    24961 tggtggtgac tggcgtattt aagatgatat tttaaaaatc agatatgaca tataagttgt
    25021 caggtcagga actagatatt ttgtgtactt gatttgcaaa cagctccggt gttggtggcg
    25081 tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc acatggatgt gggctgaggg
    25141 cgcgtcttac aaggacgttt cctcgcgccc gacccggtag ccgaagggga taagctgaag
    25201 gtaccgcgca gcggcgattt tgttcgccag agcccggggg tgcaggggga ggaggcgatt
    25261 gccctccccc tgcgcgctcc ttgcgccagt ggcaatatgt ggcttagttc atgaaaggag
    25321 gcaacaagat gatgaagctg aataagcgat tcattgtctc tgataaactg cgattccact
    25381 attatcgcag cttatcgata tcaattgcct gaatagccct tgcaatatca tgggaattat
    25441 tcaacacccg aacatgctga aataattccg ttgcttcatc gtattcttta cgcaaataac
    25501 tcaaccactg tttaatccgc gctacgtgat ataacccggt atcgccctgc ttttccagac
    25561 gggtatattt ttgcagcaaa gcaaccacct ccggccacgg cattcgcggt tcgttatatt
    25621 ttaccacccg gctcaggttg ggaatattga gcgccccgcg accaatcatc actgcgtcgc
    25681 agccgctgat cgccatgcat tgttgcgcgc tctgccagtc ccagatttca ccgttggcaa
    25741 tcaccggaat attcagccgc tggcgaatct caccaatcgc ctgccagtca atatgctccg
    25801 cgcggtaacc ctgctctttt gtccgtccat gcaccaccag ctccgtagca ccagcctgtt
    25861 gaaccgcatc ggcgatttca aatttcttct caccgctgtc ccagcccaga cgcactttca
    25921 cgctgacggg caaatgcgcc ggtacagctt cacgcatcgc ttttgcaccc tggtagatga
    25981 gttcaggatc tttgagtaac gtcgccccac caccgctacc gttgaccgtt ttcgacgggc
    26041 agccgcaatt gagatccacg ccccaggaac ctaattccac cgcacgggcg gcgttctctg
    26101 ccagccattg tgggaactga cctaacaact gaacgcgcac cagcgtaccg gatggtgtcc
    26161 ggctggcgtt ttgtaactca gggcaaatgc gatgaaagac ttttaccggc agcagttgat
    26221 ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc gttaacttcg gtcagcaatt
    26281 cacgcaccag agagtcaagc acaccctcca tcggtgccag taacacacgc atatcatcac
    26341 ccgcaaaaaa atgaggcgct atgttagcgc ctcctgtgtt tgcagacaaa ctgacttact
    26401 gattaattcg ggcaagattt ttaccctcac cctaaccctc tcccgaaggg gcgaggggac
    26461 cgcccgtgcg cctgattggg tgaagaagtg ctcagcgtgg ttgcaaacag ttatcttctt
    26521 tccagccgta caaccactcc atgccgcgat aaaattctgc ctgcgatttc cctttccaca
    26581 gcagattacg tacctggcgt tctacccctg cggcgtggta aacccgcccc atttcacggg
    26641 tcgaccagac gatgcgcgcg gtgcgcggaa ttcgtaccga ctcatacagc gcaaaggcct
    26701 gcgccgcatc accctcacac tgcgccagcg ccttacccag cgttactgca tcttccagcg
    26761 ccatacaggc tccctgcgcc atatattgcg ccaccggatg ggccgaatcg cccacaagcg
    26821 ttatgcgctt cgttccccac ttcgccaccg gttcacggtc agcggttgac cagcgtcgcc
    26881 atgaagtcgg tttatccaac atctggcgcg gacgggggtg aatcccttca aagtaagaga
    26941 gcacttcctc tttactacca tccttcacgc cccactcttc ctgctggcgg ctgtggaatg
    27001 ttaccaccag gttatactgc ttgccgccgc gcaaagggta atgcaccaag tgacaatgcg
    27061 gccccgccca cagtaccggt gcgttaatgc gcaaatcttc cggcatatcg gcacgatcga
    27121 taaccgcacg gtagacaaca tgtccggtga cacgcggcgc atcgcccaat aagctctggc
    27181 gcaccactga cttcacgccg tcacagccaa tcagaatatc gccggtccag ctattgccgc
    27241 gatcgtcaaa cacggtgaca tcgtcttccg tctcgcgaat atccacgacg ttggtggagg
    27301 tgtgatattc cacgtccgga tgcgtcagta ccgcttccca caccgaggcg tgaatatcaa
    27361 cccggtggat caccgcgtaa gggccgccga aatggtcacg aaaagcctgc ccggtttcaa
    27421 tgcacaccac ttcttcggcg ttcaccgcat ccatcatggt aatgtgatcg gtaaacaccg
    27481 cgcgctggcg ggcaacttcg ccgacgccga ggctgtccag cgccgaaaac gcatttggcc
    27541 ccaactggat gcccgcgcca atctcaccga tctcatgcgc cttttccagc agcaccactt
    27601 tgatccccag gcgggccagt gacagcgcag ttgccgcacc gccgatcccg cctcccacaa
    27661 ttactgcgcg tgtcactttc gtcattatag ttctcctggt caggctgaaa ttttatcttg
    27721 ttgattttct ggcgctgcgg caatgaacgc tggcagctgt gtgcaggcgt cgtacaccgc
    27781 tttgcagcgt ggatacccgc tcaaatcgca gttcatgcgt agcgcgttcg cccactgcgg
    27841 gacaaggcag caatctgcca gcgtcggcgt ctcgcccaca caaaattgcc ccgactggtt
    27901 ctggcgcaac agttgttcca ccgcgcttaa cccttgctgg atccagtgcg cataccaacg
    27961 ttttttttct tcttcactga cgttcagttc ttcggtcagg tagcgcagca cgcgcaagtt
    28021 attcaatgga tgaatatcac aagcgatggc atagacaatt tccagcacct tgttgcgggc
    28081 cggctcttcc tgcggcacca gcggaacttg cgggtagtgc ctctccagcc agtcaataat
    28141 cgccagcgat tgtccgagtg attcgccctc gtcagtcagc agcgtcggca ccagcccgac
    28201 cggattcatc cgccgatagg ccagttcatt ttgctgacca atacggatat tcaccccttc
    28261 ggtctggtaa ttaatgcctt tcagcgccag cgcaatgcgt acccggtacg acgctgaact
    28321 attaaaaaaa ctgtacagct tcatcatgca cctcagacaa tcttcaccgc gataggcgtt
    28381 aagccctcga cgttgcctgt gatcacatca cctttcacta ccgggccaac cccttccggc
    28441 gtaccggtga agatcagatc gcccggttgc agctcaaaga agcctgacag atagctgatg
    28501 gtttcattca ccgaccagat gagatggcga atatcgctgc gctgatgatc ttcgccgtta
    28561 acctgtaacc agattggcgc attatcaata ccgccgatat cgcgggcttt gtgcagcggc
    28621 gcgatgggcg ctgagagatc aaacgcttta ccgatttccc acgggcggcc catctggcgc
    28681 atttccattt gacggtcacg ccgagtcata tcaagacccg tggcataacc ccagacatat
    28741 tcatgggcgt gttccagcgg gatatcgctg ccttttttac caatcgccac caccagttca
    28801 atttcgtaat ggtagttatc ggtctgtgcc ggatacggca gcgccagcgt ttcaccagcg
    28861 gcaaccggaa caacggcatc cgccggtttg cagaagaaga atggcggttc acgatccgga
    28921 tcaaagccca tttcgcgggc gtgagcggca tagttacgtc cgacgcagta aacgcggcgc
    28981 acggggaatt gttcatcact gccaacaacc ggtacggtaa cgggcgcttg cggttcgaat
    29041 acgtattgag tcattttttc tctcctaaat taataacgcg cttcgcggaa cagccccagg
    29101 gcttcttgta ccggtctgtc cgaaaaacta aataaaacgg aatcttgcgt ggtctggaac
    29161 gacacgccat gccaggtcgg caccacgaag atatcttttg cactaaagct gaaggtttca
    29221 ttgccgatga tgacctgccc gctaccttcc accacatggt agatggtgct gtcagtggta
    29281 cgcgcaacgc gtgaggcaaa ccctttcggc agcagttgca ggaaagcgcc catcgacggc
    29341 atcggatagc cgccggtgac cgggttgacg tagcgcattt tgtagccatc ccactcgtcg
    29401 gcatcgccca gtcgggttaa atcgtgcagc acttcgcggc tgcggtcata acgatagtta
    29461 aagatcggcg aggagttccc cgtctgatgc cgcagcggca acatattggc agcgtaacgc
    29521 ggcagataat ctccctcttt acgcgttacc ggttgttgct cttccggata atcttcggca
    29581 aaaccgcagc ccagaatatt cactaatggc agatccagcc catcgagcca gataaccggt
    29641 tcgtcgccag ggttaccgtg atcgtgccag cgccactgcg gggtcaggat aaaatcgcct
    29701 tcattcattg gcgtgcgttc gccatcaacg gcggtaaatg cccctttgcc ttcaacaata
    29761 aaacgcagcg ccgactgatt atgacgatga ctcggtgcca cttcgcccgg catgatcagt
    29821 tgcaaaccgg catataaagt cgcggtaatg gaagactgcc cgcgcagcgc cgggttttcc
    29881 agcaccagca cgcggcgcac ggcctctttc gcaccaatca ggccgccgct ctccagtagc
    29941 agcgggcgga tctcctgata attccagtac gctggcgcac agttagcgtt gggcgttttc
    30001 ggcaccagat ggtgcagtga ctcccacagc ggggtcaggt tttgcccgga aatatgctga
    30061 taaaattgct cgcggctatt ttgattgtta tcagtcattg ggatgctcct tattcgttca
    30121 caacgcctgc gcgaggcagt gagtcaggga cagaggtcga ctggcgaacc acggtaatca
    30181 gcagggttaa catcaccgca ctgatcgccg caggcacagc gataatgaaa aacagggtgt
    30241 cgaaagagaa attcatcgcc atcatcacgc cgccagacag cgaaccaaca atcgcgccgc
    30301 agcggccgat agcatttgac cagctcacgc ccgtggcgcg actttgtgtt ggataaagtg
    30361 ttgcggtcag cgcattcagc cccacctgcg atccgctaat gccaatgcca gtgccgaaaa
    30421 tcgccagcgc catcaaccat aatccatcct ggcttaagcc aatcatcacg atgcatatag
    30481 cgcctatggc atagctcagc gccagcacgc ggaaaggatt gaacttatcc atcagcacac
    30541 ccagcgccag cgcgcctaac gtaccaccaa tctggaacgc ggcggtgacc caggacgcgt
    30601 gttgcaggtc gatgccgcga tggttcagta gcgtcggcat ccagcttgag agcaggtaaa
    30661 taatcagcag gctcataaag aacactaccc acagcatcaa cgtgatgggt aactgacgtc
    30721 ccataaacag ttgacgaatg ctgccttttg taacggaggc tgattcaatg agataaaagt
    30781 gggtatcgac ataacgctcg cgggtgatgg cgctgacggt tttcgcaatc accgcctgcg
    30841 gtagctggcg gcgcacctgc cagcgtggcg attccggcag aactaccagc agtgcgacaa
    30901 acagcatcag cggtaacacg ccccccaaca ccagaatgcc gtgccagcca atcaccggca
    30961 ccagctgcgc actgacaatc ccgccaaacg ccgaacccag cgtaaagccg caaaacatca
    31021 gcgtcaccag cgcgccgcgg cggcgggcag gcagatactc cgaggtcatg gtgatggtgt
    31081 tcggcatcgc gccgcccaga cccaggccgg tgagaaagcg caggaagacc agcgtttgca
    31141 gatccggtga gaaagccgag gcgaggctta acgcgccaaa caaaaagacg cacagctcaa
    31201 tcacccgctt gcgaccaaag cgatcggaca acgggccaca aagcagtgcg cctgcggtta
    31261 atcctagcag acccgcgcca aaaagcgggg cgagatcgcc tgcggtgagt tgccagtgag
    31321 tgcgaatatc aggggcgata aatccgatag ccgcggtgtc aaaaccatcg agcattacca
    31381 ccagaaaaca gcagatgata acgcgccact gcatcttgcc gaccggagca gcatcgataa
    31441 gtgcttgtaa atcacgtcgt tgagtcatag taaatgcctc agtgcaggta aggtagatgt
    31501 ttcttttgtt ttgtatatgt tgcgatattg tgattaaaga gatgagatgt acaatggcat
    31561 ttcgggcaga gaaataacct ggaggttatg gcaaatggcg aactgggcgc aaaaattaaa
    31621 actacatcac cttgaaatgc tggtggcgct gggtgaacag ggcaatctaa cccatgtcgc
    31681 acgaatgatg aatatcaccc aacctgcgct ttcgaaatgg ttgtcacaac tcgaagatga
    31741 gattggaatc acactttttg agcggcacag caaaggatta cgtccgactg aaggcggcaa
    31801 attgttattg caacatgccc aacggttaat caatgacatg gagcgatcgc aacatgaact
    31861 ggcccgtttt aaacaaggtg gactggtcgg cagtctgaaa attggctgtt ctcccgtcgc
    31921 cactgactgc gtatcccagg cgattttgca tttgcttacg aagatgccaa ctctacacct
    31981 gaatatcgaa gagaaggtga tgaccccatt gctgcaagat ctattggcag gttctgtcga
    32041 tgtggtcgtt gggcgaattg gcggtcgcgc attgcagctg ccactgaatt atcaggtgct
    32101 gtacaccgag ccagtatgct ttgtcgcccg ccctgagcac ccactggcga aatacgccac
    32161 cctcagctgg aatgatcttg ccaactggcg ctggatcgtc tggccgacgg gtacccccat
    32221 tcgcattagt attgataatg cgctggtaga ccacggcgta atgctgccag aaaacacgat
    32281 tgagtcggcg tcgatgaacg tcagtaccaa cctgttacaa agcagcgata tgatctctat
    32341 tttgtcatta cgcctggcgc aacgttatgc cagccaggga caactggtga tactcaacct
    32401 gccaaagata gaacagaaag gtagcgtcgg agtgttctgg cgcaataacg aaacgccttc
    32461 ttatgcttta agtcagtttc tgcacttttt acagcagaat tagtgtgctg aacggggcgc
    32521 tcgcccgagg ttacctccct aaaattcaac tatgctgaga attcttaatg tgatcggtag
    32581 cacgttttac gaattaattg tatgatgaat ccatctcatc tggggtgttg attatgagta
    32641 agacactgaa cattatctgg caatatttac gcgctttcgt cctgatttat gcctgcctgt
    32701 atgcaggcat tttcattgct tccctgctac cggtaaccat tccgggcagc atcatcggga
    32761 tgctgatcct gtttgtcctg ctggccttgc aaattcttcc ggcgaaatgg gtcaatccgg
    32821 ggtgctacgt actgattcgc tatatggcgc tattgtttgt gccgattggc gtaggcgtca
    32881 tgcaatattt tgatttgctc cgcgcacagt ttggcccggt agtggtttcc tgtgcaatca
    32941 gtacgctggt ggtttttctg gtggtgagct ggagttcgca actggtacac ggtgaacgta
    33001 aagtcgtagg tcagaaagga tcagaagaat gatggcgaat atctggtggt cattaccgct
    33061 gacgttgatt gtcttttttg ccgcccgcaa actggcggca cggtataagt ttcccctgct
    33121 taacccgttg ctggtagcaa tggtggtgat cattcctttt ttaatgctga ccggcatctc
    33181 ttacgacagt tactttaaag gtagcgaagt gctaaacgat ctgctgcaac cggcggtcgt
    33241 cgcgctggcc tatcctttat atgagcagct acaccagatc cgcgcgcgct ggaaatcgat
    33301 catcaccatc tgttttatcg gcagcgtggt tgcgatggtg acggggactt ccgtggcatt
    33361 gttgatgggc gcttcaccgg aaatcgctgc gtcaatcctg ccgaaatcag tcaccacgcc
    33421 tattgcaatg gcggttggcg gcagtattgg cggtattccg gcaatcagcg cagtttgcgt
    33481 gattttcgtc ggcatcctcg gcgcggtatt tggccataca ttgcttaatg cgatgcgtat
    33541 tcgtaccaaa gctgcgcgcg gtctggcaat ggggactgcc tcgcacgccc tcggtacagc
    33601 gcgctgcgcc gagctggatt atcaggaagg tgcatttagt tcgctagcgc tggtgttatg
    33661 cgggataatt acttcgctga tcgcaccgtt ccttttcccg attattctgg cagtaatggg
    33721 ctaaaatttg cgatgcgtcg cgcatttttg atgtaagttt cacgcgttgc ataattaatg
    33781 agattcagat cacatataaa gccataacgg gttcgtaaac tgttatccca ttacatgatt
    33841 atgaggcaac gccatgcatc cacgttttca aaccgctttt gcccaacttg cggataactt
    33901 gcaatctgca ctggaaccta ttctggcaga caagtacttc cccgctttgt tgaccgggga
    33961 gcaagtctca tcgctgaaga gcgcaacggg gctggacgaa gacgcgctgg cattcgcact
    34021 acttccgctg gcggcggcct gtgcgcgtac gccattgtcg aattttaatg ttggcgcaat
    34081 tgcgcgcggt gtgagcggaa cctggtattt tggtgccaat atggaattta ttggtgcgac
    34141 aatgcagcaa accgttcatg ccgaacaaag cgcgatcagc cacgcctggt tgagtggtga
    34201 aaaagcgctt gcagccatca ccgttaacta cacgccttgt ggtcactgcc gtcagtttat
    34261 gaatgaactg aacagcggtc tggatctgcg tattcatctg ccgggccgcg aggcacacgc
    34321 gctgcgtgac tatctgccgg atgcctttgg gccgaaagat ctggagatta aaacgctgct
    34381 gatggacgag caggatcacg gctatgcgct gactggtgat gcgctttctc aggcggcgat
    34441 tgcggcggca aaccgttcgc acatgcctta cagtaagtcg ccaagcggtg ttgcgctgga
    34501 atgtaaagac ggtcgaattt tcagcggcag ctacgctgaa aacgccgcat tcaacccgac
    34561 tctgccgccg ttgcagggag cgttaattct gttgaatctc aagggttatg attacccgga
    34621 tatccagcgc gcggttctgg cagaaaaagc cgatgcgccg ttgattcagt gggatgccac
    34681 ctccgcaacg ctgaaagcgc tcggctgtca cagtatcgac cgcgtgcttc tcgcttaagc
    34741 ctggtgccgg atgcggcgtg aacgccttgt ccggcttgcc agccctctcc tggtgtcgaa
    34801 aatcccggca aacagtttgc cgtttcttgc gcaaaaccag cgggtaaagt agcctgatgg
    34861 aaattttcct tagatcgagt ctcctgcatg ttaaagcgcg tgttcctcag cctgttagtc
    34921 cttatcggct tgctgctgtt gactgtgctc ggcctcgatc gctggatgag ctggaaaacc
    34981 gcgccttata tctacgacga attgcaggat ctcccctacc gccaggtcgg tgtggtgctc
    35041 ggaacagcaa aatattatcg tactggcgta attaatcagt attatcgcta ccgcattcaa
    35101 ggagcgatta atgcctataa cagcggtaag gtaaattatc tattactgag cggcgataac
    35161 gcattgcaaa gttataatga gccgatgacc atgcgcaaag atttaatcgc tgctggtgtc
    35221 gacccatcag atattgttct cgattacgca ggctttagta cgctggattc catcgtgcgt
    35281 acacgcaaag ttttcgatac taatgatttc attattatca cccaacgttt ccactgtgag
    35341 cgggcattat ttattgcgct gcatatgggg attcaggctc agtgttatgc cgtaccgtca
    35401 ccgaaagata tgctgtcagt acgtattcgc gaatttgccg cccgtttcgg tgcgctggct
    35461 gacctttata tttttaaacg tgaaccgcgt tttttagggc cgctggtccc tattccggct
    35521 atgcaccagg tgccggaaga tgcgcagggg tatcccgccg tcacacccga acagttactt
    35581 gaattacaaa agaaacaagg aaagtagtta tggatgtaca gcagtttttt gtcgttgccg
    35641 tttttttcct tatcccgata ttttgtttcc gcgaagcatg gaaaggctgg cgcgcaggcg
    35701 cgattgataa acgggttaaa aatgcaccgg aaccggtgta tgtctggcga gcaaaaaatc
    35761 ccggactctt tttcgcttat atggtggcat atatcggctt cggaatttta tctatcggca
    35821 tgattgttta tcttattttc tatcgttaat attttctctt ttctgttaat tattgagaat
    35881 aattattact tcactcaata agctgcggat ataacattct gaaccgcagc tatttgtgaa
    35941 tcctttcaca gtttaaattt ccccacgcgc atagccttaa tatcagcacg caatatttat
    36001 ttagcgttcg ccttaattac tcatagcatt aaggaagatc acatgccgca acaaaattat
    36061 ctggatgaac tcactccggc ttttacgcct ttactggcga ttaaagaagc ctctcgctgt
    36121 ttattatgtc acgacgctcc ctgtagtcag gcttgcccgg cacagaccga tccggggaaa
    36181 tttattcgct caatctactt tcgtaatttt aaaggcgctg ccgagacaat tcgcgaaaat
    36241 aatgccctcg gtgccgtttg tgccagagtg tgcccgacgg agaaattatg ccaaagcggt
    36301 tgtacccgtg ccggtgttga tgctcccatt gatatcggcc gcttacagcg ttttgttact
    36361 gattttgaac aacaaacagg gatggaaatt tatcagcctg gtactaaaac gctcggcaaa
    36421 gtcgcgatta ttggcgcagg tcctgccgga ttgcaggcca gtgtgacact gacaaaccag
    36481 ggttatgacg tcacgattta tgagaaagaa gcgcaccccg gtggctggtt gcgtaacggt
    36541 attccgcaat tccggttacc gcaatcagtg ctggatgcag agatcgcccg tattgaaaaa
    36601 atgggcgtga ccattaagtg caataacgaa atcggtaaaa cactcaccct ggagcaactg
    36661 aaagcggaaa accgcgcggt actggtcacc gtggggttat caagcggttc cgggctaccg
    36721 ctgtttgagc atagcgacgt tgagattgcc gtcgacttcc tgctacgtgc acgacaggcg
    36781 caaggcgata tcagcattcc acaaagcgca ttaattatcg gcggcggtga tgtcgcgatg
    36841 gacgtagcca gcacgctgaa agttctcggc tgtcaggcgg taacctgcat agcgcgtgaa
    36901 gagttagatg agtttccggc aagcgaaaaa gagtttgcca gcgcccggga gctgggcgtt
    36961 tcgatcatcg atggattcac gccagtagcc gtggaaggca ataaagtcac gtttaagcat
    37021 gtacggttat cgggcgaact gacgatggtg gcagataaaa ttattctcgc cgtcggtcag
    37081 catgccagac tggatgcctt tgcggagtta gagcgcagcg taacaccatc aaaacacaaa
    37141 attatcagac ccgcgatccg caagtctttg ctgctggcga tattgttgag ggtgacaaaa
    37201 ccgtggtcta tgccgtgaaa accgggaaag aagccgccga ggcgattcat cactatttag
    37261 agggagcttg ctcatgttaa cgaaagatct ttcgattact ttttgcggcg tgaagtttcc
    37321 caacccgttc tgcctctctt cttcgccggt aggcaactgc tatgagatgt gtgccaaagc
    37381 ctacgacaca ggttggggcg gcgtggtgtt taaaacgatc ggctttttta tcgccaacga
    37441 agtctcgccg cgttttgatc atctggtgaa agaagatacc ggttttatcg gcttcaaaaa
    37501 tatggagcag attgctgaac atccgttgga agagaatctg gccgccctgc gtcggctgaa
    37561 ggaagattac ccggacaaag tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg
    37621 ggaggagctg gcgcgcctgg tgcaagaagc tggcgcggat atgatcgagt gtaacttctc
    37681 ctgtccgcaa atgacttctc atgcgatggg tagcgatgtc gggcaaagcc cggagctggt
    37741 agaaaaatat tgtcgggcag tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac
    37801 gccgaatatc ggtgatatgt gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg
    37861 cattgcggcg attaacaccg ttaaatccat caccaatatc gatcttaatc agaaaatcgg
    37921 tatgccgatc gttaacggaa aatcgagtat ttccggatat tccggtaaag cggtaaaacc
    37981 gatcgccctg cgcttcattc agcaaatgcg cacccatcca gaactgcgcg atttcccaat
    38041 cagcggtatc ggcggcattg aaacctggga ggatgcggcg gagtttttat tgctcggcgc
    38101 agcaacgtta caggtgacca ccggcattat gcagtacggg tatcggatag tggaagatat
    38161 ggcgagcggg ttgtcgcatt atctcgccga tcagggattt gattcgttgc aggagatggt
    38221 aggtctggcg aataacaata ttgtcccggc ggaagattta gaccgcagtt atattgtcta
    38281 tccccgtatc aatcttgata aatgtgttgg ctgtggacgc tgttatattt cctgttacga
    38341 cggcggtcac caggcgatgg aatggagcga gaaaacccgc acaccgcatt gtaataccga
    38401 gaaatgtgtg ggttgtctgc tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg
    38461 ggaagtgaag tttaagaaag gcgagaaaga acacccggta acgttgtaaa accagagacg
    38521 catccggcat ttggttcctg tcgccggatg cggcttgaac gccttatccg gcctacaaaa
    38581 ccatacaaat cagtatgttg agctcattgt aggccggata aaacgcgcca gcgttgcatc
    38641 cggcatttgg ctcctgtcgc cggatgcggc gtgaacgcct tatccggcct acaaaaccac
    38701 acagatcatg atgttatgtg cgtttatgac cgaatgcgga ccacaatcat atcacttctt
    38761 acgcgcgtac ttcagtgaat ccagcgctac ggcgaagata ataatcgccc ctttgatgat
    38821 gtactgccag tatgggttta cgccgatata cgtcaggcca tagttgatga cggtaaaaat
    38881 aattaccccg gtcaccacgc caatcaccgt ccccacaccg ccgctgaacg atacaccgcc
    38941 taccacgcac gccgcgatag catccagttc atacataaag ccgaggttgt tggtggcaga
    39001 gccgatacgt ccggcttcta acatcccgcc gaaggcatag aacacaccag acaacgcgta
    39061 gatcatcagc aggttcaggc cgacgttgac accagatact tttgccgctt ccgggttacc
    39121 gccaatggca aaaatgttct taccgaagcg ggttttgttc cacaacaccc agacgaacgc
    39181 caccgcaatc aacgcgtaga aggtgatgta agagagacgg aaactcccca gcgcgacaaa
    39241 gccctgagca aaggtagaga agccactgtc aaaaccagaa attggcgacg ccccgacaaa
    39301 gtcgtaatag agcgagttga tgccatagac gatgatcatc gtgccgagcg tggtaatgaa
    39361 cggcgtcacg ttgagataag cgataatcag accgttgatc aaaccgatca ccgcaccaat
    39421 ggcacagaca atcagaataa ccagcgcaat cggcatcgtc gccatttctg ggaacacttt
    39481 gttggcgtta tccatggact gcaataacgt cgccgctact actgccgcca gccccacctg
    39541 gcgacctgca gaaaggtcgg tcccctgggt gacaattaac cctgccacgc cgagcgcgat
    39601 aataatacgc accgatgact gggtgagaat attacttaag ttcaacagac ttaaaaatgt
    39661 tgggtcctgg aaaataataa tcgccagcaa aactaaaaga acgacgtaaa taccgccctc
    39721 tttcaggtaa gtaagaaaac ttttcttatt taacgcactc atgggaagcc cctaatctta
    39781 aaggtgcaaa gacgcaagac gcagaatttc gttttgcgtt gttgtttttg tgtcgacaat
    39841 tccggaaacg agaccgttgc tcatgaccag aatacggtct gttatcccta acaactcagg
    39901 catttcagag gagataataa taatcccctt gcctttcttc gccagttcgg caattaactg
    39961 atagatctca aacttcgccc cgacatcaat accgcgcgtc ggttcatcga gcattaatat
    40021 ttctggttgc gttagtagcc agcgaccaat aatcaccttt tgctgattac caccggagag
    40081 cgaaccaatt tgcgtccgat gacccggcgt ttttacccgc atcgaatcaa tcacccactg
    40141 ggtatcgctt ttcatccgcg agttatccag taaaccaact ttatttttgt agttgcgaat
    40201 attggaaatt aacgagttaa aaccgatatc cagataggca taaattcccg ttgagcggcg
    40261 ctcctcagtt accagagcaa atccgtggtt tatggcttca ttggcattat ggttattgat
    40321 ctttttgccg tgcaacgtaa tggtgccagc cgatttctcg cgaataccaa ataacgtctc
    40381 aacaatatcg gtacgtttcg cccccaccag accggcaata ccgaggatct cccctttatg
    40441 cagatcaaac gagacatcgc gaatcgacgg ctggcgcagt gacgtcaggt tacgcacctc
    40501 gaggatgact tcgcccggct tgttttcttt gtcaggaaaa cgctggttaa gagaacgccc
    40561 aaccatcatg gcgataatct tgtccatcgt cagccctgcc agcggttcgg tggcgatcca
    40621 ctgaccgtcg cgcaatacgg taacttcatc acataactgg aagatttctt ccattttatg
    40681 cgagatataa acaataccgc agccgcgctc ttttaattta cgaataatag tgaacagatg
    40741 attgacctct ttttcggtta acgaagaagt cggttcatcc ataatcacaa ttttcgcgtt
    40801 ataggaaaat gctttggcga tttcgatcat ctgcatttgc gaaacagata atgtgccgac
    40861 gcgcgcacgc ggatcgatat caatatccag ttcatcaaaa atcgctttgg tttcgcggta
    40921 cattttgtcc tgatcgacaa acatgccttt ggtggggtat cgcccaagcc acatgttgtc
    40981 catcaccgaa cgttgtaata ccaggtttaa ctcctggtgt accatcgaaa taccattttc
    41041 cagtgcttct ttggccgaat ggaaatcgat ctctttaccc tggaataaaa tggagccgga
    41101 gtctttttga taaataccaa acaggcattt taataatgtc gattttcctg caccgttttc
    41161 ccccattaat gcatggatag aatgtggccg gacttttaaa ttaacgttat caagtgcctt
    41221 aacaccagga aaagacttgt tgataccgct catttccaac aagtattccc cggaagacgg
    41281 agtcgttgag ctgaccatat aattttacct tgttggccat acaataaggg cgcagtaata
    41341 gactgcgccc aatcagtctt atttcttgct gaattcagcc aggttgtctt tatctacgcc
    41401 aacataaggt acgcggacca ctttgttgtc gattttccag ttggtgccat cagccgcacc
    41461 tttaccatcg gccaggtttt tcgccagatc aaaggtcgct ttcgcctggt tgttagcatc
    41521 gttcagtacg gtgcccgcca gtgcaccgga tttcaccagc gccagcgctt ctggcagagc
    41581 atcgacgcca aacaccggaa tgctggactt gttgtgtgct ttcagtgctt ctaccgcgcc
    41641 cattgccatc gcatcgttgt tggcgataac cacttcgatt ttgttggcgt tcgggccaga
    41701 cagccaggcg tccatcttat ctttcgcctg agcggtatcc cacatagcgg tatctaactg
    41761 taactgttca gttttaatgc ctttgtcgtt cagctcttta atcacgtaag tggtacgtgc
    41821 ttctgcatcc ggatggcccg gttcaccttt cagcagtacg aattgaatct gaccgtcttt
    41881 attcagatcc caaccctgat tcgccgccca gtgtttagca atcaaatcgc cctgaataat
    41941 gccggactct ttggagtcag tgccaacgta gtaggctttg tcgtagctat ccagcgcctt
    42001 acgagacggt tctttgttga agaaaaccac tggcacgttt tgcccacgcg ctttctcaat
    42061 caccgtaccc gcagctgccg ggtcaaccag gttgattgcc agtgccttca cccctttcgc
    42121 cagcaatacg tcgatctgat cgttctgctt ggactggtca ttctgagaat cattcatcag
    42181 cagctgaaca tctggcgcgg ctttcgcatc ttgctcaata gccttgcgca ctacagacat
    42241 aaagttatcg tcgtacttat agattgttac accaatgcga gtatcagcag cgtgtgcagc
    42301 ggcaccgaat aacatgctgg ccatcacagc agacagggtt aacaccttct tattcatggt
    42361 atctccggtt tttcttatgc agggtagtgc ttgagataaa tgctcggcgg ggcagtagag
    42421 ttaatgaagt gttactgaac gccgaagctc actttgtaaa attcgttctt ccatgcccgg
    42481 taacgctcca gaaaacggct ttaattgttg ttatgacgct attacctcgg tgaaagtgat
    42541 tattcactgt tacatacggg ttacaacgtt aaaacggtgc aatcatagct atcacattgt
    42601 taagatactg tgaaatcact cacagattga aagcggttac atcgccagat ttgttgagtt
    42661 agtgatcgcc gccgcattct ggcgcgttgc gacagaatgg cgacgcacta acgtcggcat
    42721 aaaacagtgg ctggcacgag gatcaatatt gcctgctgca ccctgcaagg ccagttcggt
    42781 ggctaattta gccattgaag caatgggata acgcacggtc gttaattgcg ggtcggtgta
    42841 acgggcgatg ggaatatcat cgaaaccgat gattgagaga tgtaacggaa tcgcaatgcc
    42901 attatctttt aatgctgtca gtgcgccagc agccatattg tcgttataag caaatacagc
    42961 ggtaagttgt agattgcgcc ccagcagttc aaccatcgcc gcctcaccgc ccggcatgtc
    43021 cggcgtgcca gtgccaatcc agctttccgg cggaataata tcctgctctt tcaacgcact
    43081 catccagcct gctttacgca tggcgtcatc ttcaatgccg tgactggaag aaagataacc
    43141 aatacgttga tgaccgttat tcagcaacat gcgcgttgcc attcgggcac cgctgagatt
    43201 atccaggcaa acgcaacgat gggcgtaccc cggtacaacg cggttgatta acaccatacc
    43261 gggaatgtta tccataaatt gtgccagttc atcgtcactc aatgcttttg agtgaacaat
    43321 caacgcatta caacgctggc gaattaacac ctcaatggcg tgacgctctt tttccgcttc
    43381 atgatagcta ttgccgatta gcacgtattt ctgatgctgc tgagcgacca gatccaccgc
    43441 ttttaccagc gcgccgaaaa acgcatcaga aacgtccatc accaccacgc caatggtgtc
    43501 gctaacctga gttgccagcg cctgggcatt ggcgtttggc cgataatcca gctcactcac
    43561 ggctttcatt actgcttcac gcgtgtcggc actgaccagc gtgctgttat tgagcacccg
    43621 ggaaaccgtt gccacagaga cgccagcctg acgcgctaca tcacgaatgg tgatcatatt
    43681 cactacctgt tatgagattg cagtaaatga ctgcttgctg gcggctattt tgtcagcact
    43741 gaatacagga cttcgtgaat cgagtcacag caatggaaac ggttacagcc gttttgttaa
    43801 cgaatgtgat ccagatcgtt atcttgatgt tttagatatt gccggtccgg cagcacgcaa
    43861 agttaactga cgccagagcc attccaccgg cccctggcgg aagtaacgca gccagataac
    43921 agagaagagg atattcgcca gccataccgg aataacaaac gccagcagct ccaggcggtc
    43981 aaaatgcata aacaaaccga ggtggtaaaa aagcgtggta caaatcagcg tttgcaatag
    44041 atagttggtc agcgccatcc gtccgacgca ggcgatcgca agcaccagct taaagcggct
    44101 caattgcggc cagaaaccat aaaacagcga cgcatagcca atcgcctgaa acggtgcact
    44161 cagttcccgc ggcatctgaa gtaagaaggc gcaccagcga tatgcccagt ccagccgcca
    44221 ttgcagggcg atggcaggaa ggttaatgat caccccaatc gccaccagca caaaaccagt
    44281 acgacgatag tgacgtaagc tgaactgccc tttcagccag ccactgcgca tcaatgcggc
    44341 accaatgagc atcatcccag ccagttgcca gccatactgt gcgcccagtg ccagtaaact
    44401 gttcccaaca ccatcggcac gattactgat cgcttcaacg ccgccgtgaa gcttccagta
    44461 tttttcatac aaaatagccg atgcatccgg cgtccaggcg cggctggtct gggtatcgga
    44521 aatcaacccc aataacagca aaacgccaag ccccaccaga taaagcatga cgccggtatt
    44581 aaacaggctt tttaccgatg gcgcatcgcg caccagccgc cagcagatta agcccaccag
    44641 cccgtaagcc agcagaatat cgccgtccca gaacaataaa ccgtgaataa agcccagcaa
    44701 gactaacagc gttaaccgcg actggatcca gcgtctgcca cggggcagca acatttgcag
    44761 gcccgcacca aacagcagcg caaaaagcgt gaggaatttc acctggccga tgagatcgag
    44821 aaatgcccag gtccatgcat cctgcggcgt aatagcgccg taccaggcgg gattaagata
    44881 agccgccttt ggtagcccaa aggcgctgat gtttaatagc aggatcccca gaatggcgac
    44941 gccgcgaaca aaatcgagcg tgacgttgcg ctccatggtt cctgccttta aatcagttgt
    45001 gatgacgcac agcgcgcaga aactcgtggc gcgtattctg actggatttg aacaatccac
    45061 caagagaggt cgttgtcgtg gcactggttg catcgcggat gccacgcgcc ttcacgcaat
    45121 aatgcaccgc gtcgatagag acagccacgt tattggtgcc cagcagcgtt tgtagcgcaa
    45181 tgagaatttg ctgcgtcaga cgttcctgca cctgcggacg ctgggcaaag aactgcacaa
    45241 tacggttaat ttttgacaga ccgatcaccg aatctttcgg gatataagcc accgtcgctt
    45301 tgccatcgat ggtaacaaaa tggtgttcac aggtactggt cagagtgata tcgcgcacgg
    45361 tgaccatttc atcgaccttc attttgtttt caatgagggt gattttcggg aagttggcgt
    45421 aatccagacc ggagaaaatt tcatcgacat acattttagc gatgcgatgc ggcgtttcca
    45481 tcaaactgtc atcagccagg tcgagattca gcagctgcat aatttcggtc atatgaccag
    45541 caataaggct tttgcgcgtt tcgttatcca tttcatgcac gggcgggcgc agcggtgttt
    45601 ccagtcctcg cgcaactaac gcttcatgaa ccagggccgc ttctttactg agtgatggca
    45661 tttatgattt ctcctgcagg tgtgacgcct ccgccctgcg tgggggcaaa gttattaagc
    45721 tgatttacag cctgattatt gtgcgtgagg cggcgcacat aatccagtat tcacagcgat
    45781 aattattgta attgccgctg cctttcatca gcagatgtta aaacatcgtt atgcaaatac
    45841 ggaagtgaaa gttactcaca gcacattgaa taaacggtat gatgaagaaa ttgcaaacaa
    45901 cacaacaagg agccacgcat ggaaatgctc gaagagcacc gctgttttga aggctggcag
    45961 caacgctggc gacacgactc cagtacctta aactgcccga tgacgttcag tatctttctc
    46021 ccaccccctc gtgatcacac cccgccacca gtgctgtact ggctttccgg attaacctgc
    46081 aatgacgaga acttcaccac caaggcgggt gcccagcgcg tggcggcgga actggggatt
    46141 gtactggtga tgccagacac cagcccgcgc ggcgaaaagg ttgccaacga cgatggctac
    46201 gatttaggcc agggcgcagg cttttatctt aatgccacgc aaccgccgtg ggcgacgcat
    46261 taccggatgt atgattatct gcgcgatgaa ttaccggcgc tggttcagtc gcaatttaat
    46321 gtcagcgacc gctgcgctat tagcggtcat tcaatgggtg gtcacggtgc gctgattatg
    46381 gcgctgaaaa acccaggcaa atacaccagc gtttcggcct ttgcgccaat tgtgaatccg
    46441 tgcagcgtcc cgtggggaat caaagcgttt agccgctatt taggtgagga caaaaatgca
    46501 tggctggaat gggacagttg cgcgctgatg tatgccagta acgcgcagga tgcgatcccg
    46561 acgcttatcg atcagggtga taacgatcag tttcttgctg accaactgca acctgcagta
    46621 cttgcagaag ccgcgcgcca gaaagcatgg ccgatgacgc tgcgtattca gccgggatat
    46681 gatcacagtt actacttcat cgcctctttt atagaggatc acctgcgctt ccatgcgcag
    46741 tatttactga agtgaaagtc cgcccggttc gccgggcatc ttctcatcag aagcgataat
    46801 ccactgccat aaagtaacga cgtccgtctt cgttatagct gtagtcgtca cgactgagat
    46861 ccttgtcgcc aaggttcagc acgcctgcgc gcagtttgac gtctttagtc acctgccagg
    46921 ccgcgccggt attccagatg gtataaccgc ccggtgtttt agccgtcgcg ctgtcggctc
    46981 gtttctgccc ggtatagtga ccagaaacat agaatgacca gtcttccagc gccagcggtt
    47041 tccagtccag cgtaccgtta gcggtatgga atggcagatc ggatagcggt ttgttttcgc
    47101 cgttgctgac atcacgacca tcgttgtagg tgtagttgag tgacagtttc cattcatcgt
    47161 tgaacggaat tttcagctcg gtttccacgc cctgaatacg tgctttgtta acgttgtagt
    47221 agctaaatac cggcacaccg ttcgcagtaa aaccaacaaa gttttggtag cccggtgcag
    47281 cattgacgtc agacgtacgg ctgatgctga tacgatcttt cacatcgtta cggaaaacgg
    47341 taacgctgga ttcaacccct tccagccagc cttcttcacc catgtagtaa agccccagct
    47401 cccaactttc gctggtttct ggtttcagat ccgggctacc cacaatctta catgcgccac
    47461 ggcaggaatt gctcgtccag tcagggctaa gttgcaacag agaaggtgct ttaaatgccg
    47521 tcgcccagcc ccctttcacc gttacggtgt cggtggcgtt ataaaccagg taggcacgcg
    47581 gactccagtg ttcaccgtag gtttcgtgat cgtccatacg cacgccggtc gtcagcgcca
    47641 gcggctcgaa gatccgccat tcatcttcca caaacagcgc gtactggctg gcagacgttt
    47701 tggagctggt tcccccggtc aggttcaccg catcgctaag tttgtcgtga cgccattcac
    47761 cgccaaccgt gagaaactga ttaatcgccg tcagcggcaa cgtgtatttg ccgtcgaccg
    47821 tattgctttc ggaagttatc gggctgctgt tgccagggtt tttgttctcg actttctcac
    47881 cgtagtattt cagttcgctg gtgccgtaat cccaacgccc attatggctg acggagtagt
    47941 tctggcgttc caagcggttt ttgtccagcg agtcggaatc acgatcctga cggtcgaaac
    48001 cgtatccggc agtaaaatcg tgattttgat tcggtgtcca ggcaaattcg acattgccgt
    48061 cgcggctgga gaatccttca atacgcggcg tttctccggt atcggtggtc gttgagtttt
    48121 gcgggtcatc cttttcacgt tttgccaggc tgccgtaagc tttcattccc agcacaccat
    48181 caattaatgg tccactggta aagaactgac cgttataggt atcaccgcga tcgcgatgtt
    48241 cctgaatggt ggtatcgacg gtaacggtac ccgaccattt ctgaccgatt tttttggtga
    48301 tgatattcac tacaccgccg agcgcatcgg aaccgtacag cgacgacatc gggccacgga
    48361 ccacttcaat acgttcgatg gaatcgaccg ggatccagtt cagatcgaaa tcattgtggc
    48421 ggaagacggc attgcgggag ttaacgcgtt taccgtcgac aagaatcagg gtgtagctgc
    48481 tgtccagacc acgaatacta acgcccttac ggttatcccc ttcgttcgtc agttgtacgc
    48541 caggcacttc tttgaggaca tccttcagat tctgtaccgg ttttcgctgc aggtcttcct
    48601 gggtaatgac gctgatactg gcgggtgcat ctttaaggtt ttgttcaacg gaagatgcag
    48661 tgacaaccat cgtttcgcca tcatcatcga ccgctaacac aggccatgca caagaaatag
    48721 cggacaaaca cagcccgacc cgtacgaaag ggttcaacct aaacattcca tatctccatg
    48781 aggtaactac gaaaataaaa tgggttatcg ctcacatctt cttcacgtcc ccttgcgtac
    48841 ggcagcatcg cggtggactt atttgtatgc aggtgatcat cccagaaagc cggaccagcc
    48901 tctgatttgt cagccttttt tgattgcggc gtaacgataa tgcaaacgat aacaattatc
    48961 aatccaaatg ttaaatttta tatccgcatg atttgtcagg gaaatggaat tgaaaaagcc
    49021 ctctcggttg agagggctta gcaaggaagg gagaaactta tttcttatcg ttctgcggga
    49081 acttcatttc gctgtagcgt acgaagtggg ttcctttaat cagcttgtag ccgaaccaaa
    49141 taatcaggaa cagcgggata ccaatatacg ttgccgctac gccgccccag tcaatggtat
    49201 ctttaaggaa cgcttcgtag ttctggccca aagtgataat cagacacaga atgaatgcga
    49261 agatcggccc caatgggaag aaacctgaac ggtacggcag atcgttaatg tcgtgtccct
    49321 gcaatacgta accgcgacgg aagcgatagt ggctaatggc aatccccagc caggcgataa
    49381 aacccgtcat cccggaggtg ttcagcagcc acaggtatac cgtctggttg ccaaacatgg
    49441 aggtcaggaa gcacagaccg gcaatcaccg tcgtcgcata cagcgcatta cgcggcacgc
    49501 caccacgcga cagtttagcg aaaatgcgcg gcgctttacc gtcacacgcc agggtgtaca
    49561 gcatacgagt agacgcatac ataccggagt tacccgctga cagcaccgcc gtcagaataa
    49621 ctgcgttcat caccgccgcc gcagagagca gacccgcgtg ctggaacacc agggtgaacg
    49681 gactaacgct gatgtcttta acatcgttac gtagcaggct tggatcggtg tacggaataa
    49741 tcaggctgat aatcaggatc gcgaacacat agaacaacag gattcgccag aacacctgac
    49801 gtaccgcgcg tggaatgttt ttccccggat cttcggactc gcctgcagca ataccgatca
    49861 gctcggttcc ctggaaagag aagccgacaa tcatagctac gccgatcatc gccgcaaaac
    49921 caccagcaaa cggcgcttcg ccgattgtcc agttgctcca gcccgcaggc tgcgcgcctt
    49981 tgaagatacc gataatcatc agcacgccaa cgatgataaa gacaataact gtcgtgactt
    50041 tgatcagtga gaaccagtat tccgcttcac caaagccgcg aacagagatg tagttcagca
    50101 ggaagataac gccgaggaac aacgcactcc agatccagcc cggtgtatcc gggaaccacc
    50161 agctcatgac cagctgagct gcaaccaggt caacggcaat agtcaccgcc cagttgtacc
    50221 agtagttcca gcccagcgcg aagccaaagc cctcttcaac atagttctga ccgtaagtgg
    50281 caaacgaacc ggaaaccggc atatatgcag ccagttcacc gagactggtc atcaggaagt
    50341 aaaccatcag gccaatcagc atatacgaga gcaatgcccc gcccggacct gcctgagaaa
    50401 tcgttgcgcc agaggcaaca aaaagacctg taccgatgga accgccaatg gcaatcatcg
    50461 tcaggtgacg cgcctttaat tcacggcgta agcccggcgc ttctgtggtt ttagtttcgg
    50521 aaaccataca aaaatgctat ccatctaaaa aatgaggcgc gattgtagca gacgattggc
    50581 aatccttccg gcagaaatac acggttataa gacaccttca tgatcgccca gggattataa
    50641 gtaaagcagc caatctcttt tctggcgaat gcatgctgaa ggcgctgcgt cacgaaatcg
    50701 acacagcacc agcatgttct tgtacagcaa cttaacgcgg cacatttgcg ggatcgcaat
    50761 agtccagaaa gcgccgtagc gcgttggaaa ggtgtttttg ccgatgatgt atacgccaca
    50821 acgtacgcat caggcgcggc agagggaccg caacttcact taatgtgcct gcctgcaatt
    50881 ggtcttcaat cacccgtcgc gacaggcaac taattcccaa cccatgacgc accgcatgtt
    50941 tgattgcctc ggagttacct aattccatcg ccatctcaaa cttcggtaaa tgtgacagca
    51001 acagataatc gacaatctcc cgcgtgccgg aaccgcgttc acgcaggatc cacggcgcag
    51061 cggccagctg ttctaaggtg accggaccac gggccaacgg cgaagtcggc gcggcgaaaa
    51121 ccaccagctc gtcttccagc cacggttcag aaatgatttc agtgctgtgg cacggtcctt
    51181 caataaagcc aatatcaacg cggaaatcca gcaccgcttg catcacgtcc tggctgttcc
    51241 caacgctaag ttcaatcggc aactgcggat aatcatggcg ataacgggcg ataactgcag
    51301 gcagaatgta gttaccgatg gtactactgg catagatacg aatcgcgccg ttgtcttcgc
    51361 gaaacagttg ttcgatttca accgcctgtt caagcaatgc caacgcacgc ggatagagca
    51421 gccgcccgtg ttcatttaca accagtcttt tccccacgcg atcaaacagt tgcacgccaa
    51481 gctgcccttc caggtcggtc aaggctgcgc tcactgctga ttgcgacaac gccagcatca
    51541 ccgacgcctg ggtggttgat ccacttttca atacttctgc aaaaacttcc aactgccgga
    51601 gggtgatgtg catagtcgct taccacttat aaagattaat tataaatata taatcaattt
    51661 tatttttaaa ccagttagtc gtaaccttat gcccggttaa aggagagggt tatgacgaat
    51721 atcaccttac agaaacaaca tcgtacactg tggcatttta ttccggggtt agccctgagt
    51781 gcagttatca ccggggtcgc cctgtggggt ggttccattc ccgcggttgc gggtgccggg
    51841 tttagtgccc tcaccctcgc aatcttgttg gggatggttt taggcaacac catctatccg
    51901 cacatctgga aaagctgtga cggtggtgtg ctgtttgcca agcaatattt attacgtctg
    51961 ggtatcattc tttatggctt ccgtctgacg ttctcgcaaa ttgccgatgt cggtatcagt
    52021 gggatcatca ttgacgtgtt gacgctgtcc agtaccttcc tgctggcttg cttcctgggg
    52081 cagaaagtgt ttggtctgga taagcacacc agctggttga tcggtgccgg tagcagtatc
    52141 tgtggtgctg ccgcggtact ggcgactgag ccggtagtga aagcggaagc cagtaaagta
    52201 accgtggctg ttgcaaccgt tgttatcttc gggaccgtcg cgattttcct ctacccggcg
    52261 atatatccgc tgatgtctca atggtttagt ccggaaactt tcggtatcta cattggttct
    52321 actgtgcacg aagtggcgca ggtggtggcg gcaggtcatg ccatcagccc ggatgcagaa
    52381 aacgcagcag ttatttccaa aatgctgcgc gtgatgatgc tggctccgtt cctcatcctg
    52441 ctggcggcgc gtgttaaaca gctgtctggg gcgaacagcg gcgagaaaag caaaatcact
    52501 attccgtggt ttgccatctt gttcatcgta gttgccatct ttaactcgtt ccacctgtta
    52561 ccgcagagcg tggtgaacat gctggtaacg ctggatacct tcctgctggc aatggcgatg
    52621 gcggcgctgg gtctgaccac tcacgtcagc gcgctgaaaa aagctggggc gaaacctctg
    52681 ctgatggcac tggtactgtt tgcctggctg attgttggtg gtggtgcgat taactatgtg
    52741 attcaaagcg tcatcgcata aaccactaca tcttgctcct gttaacccgc tatcattacc
    52801 gttttcctcc agcgggttta acaggagtcc tcgcatgaaa tacattggag cgcacgttag
    52861 tgctgctggc ggtctggcaa atgccgcaat tcgcgccgcc gaaatcgacg caaccgcgtt
    52921 tgccttgttc accaaaaacc aacgtcagtg gcgtgccgca ccgctcacga cgcaaaccat
    52981 cgatgaattc aaagccgcct gtgaaaaata tcactacaca tcggcgcaaa ttcttcccca
    53041 cgacagttat ctgattaacc tcggacatcc ggtcactgaa gctctggaaa aatcgcgcga
    53101 tgcctttata gatgaaatgc agcgttgcga acagctgggg ctttctttgc tcaacttcca
    53161 ccctggcagc catctgatgc agatttcaga agaggattgc cttgcgcgta ttgccgaatc
    53221 cataaacatt gcgctggata aaactcaagg tgtgaccgcg gtgattgaaa acaccgccgg
    53281 tcagggcagt aacttagggt ttaaattcga acatctcgcg gcgattatcg acggcgtgga
    53341 agataaatcc cgtgtcggcg tctgcattga tacctgccat gctttcgctg ccgggtatga
    53401 tttgcgtact ccagccgaat gcgagaaaac gttcgctgat tttgcccgta ttgtcggctt
    53461 taagtatctg cgcgggatgc accttaacga tgcgaaaagc acctttggca gccgcgttga
    53521 ccgccatcat agcctcggtg aaggcaatat cggtcatgat gcgttccgct ggatcatgca
    53581 ggacgaccgt ttcgacggca ttccgctgat ccttgaaacc atcaacccgg atatctgggc
    53641 agaagagatc gcctggctga aagcgcaaca aactgaaaaa gcggtagcct gaagatgaat
    53701 aaccgggaaa aggagatcct tgcaatttta cggcgtaacc cgctgattca gcagaacgaa
    53761 attgcggaca tgctgcaaat cagccgttcg cgcgttgcgg cgcatattat ggatttaatg
    53821 cgcaaaggcc ggattaaagg caaaggttac attctcaccg agcaggaata ctgcgtagtg
    53881 gtggggacaa tcaatatgga tattcgcggg atggcggata tccattaccc gcaagcggct
    53941 tctcatcccg gtacaattca ttgctcggca ggcggcgttg gacgcaatat cgcccacaat
    54001 ctggcgctgt taggccgcga cgtccatttg ctttcagtga ttggcgatga cttttatggc
    54061 gaaatgctcc tggaagaaac gcgccgtgcc ggcgtgaatg tctccggctg cgttcgtttg
    54121 catggtcaaa gcacatcgac gtatctggca attgccaatc gagacgatca aaccgtgctg
    54181 gcgattaacg atacccatct gctggaacag ttgacaccgc aactactgaa cgggtcgcgc
    54241 gatttacttc gtcatgcggg cgtggtactg gctgattgca acctgacagc cgaggcgctg
    54301 gaatgggtct ttaccctcgc tgatcaaatc ccggtgtttg tcgataccgt ttcagaattc
    54361 aaagcgggca aaatcaaacc ctggctggcg catattcaca ccctgaaacc cactttaccg
    54421 gagctggaaa ttttatgggg acaggcgatc accagcgatg ctgaccgtaa tgccgcagtg
    54481 aatgcgttgc atcagcaagg tgttcagcaa ctgtttgttt atttgcccga tgagtcagtt
    54541 tattgcagcg aaaaggatgg tgaacaattt ttgctgactg cgccagcgca tacgacggta
    54601 gacagttttg gtgctgacga tggttttatg gcgggtctgg tatatagctt tctggaggga
    54661 aacaatttcc gtgacagcgc ccgttttgcg atggcctgcg cggcaatttc acgcgccagc
    54721 ggcagcttaa acaaccctac cctgtctgcc gataatgcgc tgtcattagt gccaatggtg
    54781 taacaatgtt gccggattcg acgcttgacc gcatcttatc cggctcataa atcgcacact
    54841 acgctaaacc aataaagaac ccggcaatgg tggcactcat caggttagaa agtgtcgccg
    54901 ccgccagcgc gcgtaaacca agctgggcga tttccggcgc acggtgtggc gcaaccgcag
    54961 aaaacgcccc caccaccacc ccgatagaac caaagttagc gaaaccgcac aacgcgaagg
    55021 aaataatcgc cacggtttta gcatccagag tgccaccagt ttgcagatag ggtgagaaat
    55081 tgaggtaagc gacaaattca tttattgcca gtttctgtcc aatcaaactc ccggcaagat
    55141 tcgcatcact ccagtcaaca cccatcaccc acgccagtgg ggccaacagg taacctaaaa
    55201 tggactccag cgaggcatgt tcaaaaccaa accagccgcc aacgccgccg ataataccgt
    55261 taattaacgc aatgatggcg acgaatgcca tcactactgt cgccacacct gcggcgattt
    55321 tcagcccggt cattgcccct gtcgcggcgg cttcaataat gctttttggc ggtgtttcgg
    55381 tgaaagagag gttattaaaa gaaacctgcg aagattccgt agccgggctt aacaggcggg
    55441 caaacaagat tccgcctggg atcgccatta acgatgccgc cagcaaatat tccacaggca
    55501 cgcccagtgc ggcgtaacca atcattgtcg aaccggcaat tgaggccatg ccactacaaa
    55561 tcgctgtaaa taattcattg cgattcagcc gatcgataaa gggtttgacg attgccggaa
    55621 tttcgttttg cccgaggaaa atggtagtga ccgcgacgaa tgactcgatc ttgctgatat
    55681 ttaatgcttt ctggaagata ccgccgagaa tgcgaattaa gatccccatc acaccgatgt
    55741 agtagagaat actcaccagc gcggtgacga agataattgc cggtaatact ctgaaaccaa
    55801 agataaatcc tgcgccatca aacagcgtgt ccattttcgg cccgaccagc gaaccgaaga
    55861 taaatgcgct acctgcatcg ctgtacgcca tcactttatg cacgccaaaa gcgacttttt
    55921 cagcgaccca acgccctggc ggtaaccaaa gcataatccc gccaatcacg acctgtaaca
    55981 ctaacgccgc gccaacggta cgcaggctga tcttcttctt gtttactgac agtaaaaacg
    56041 caatcgtcag caataccacc attcccagaa cacttctcat gacatccata gttattatct
    56101 cttcatgtca tattgggcga cggctttctg cccctcagaa atagccgggc ctgcatcttg
    56161 cgatactgac ccggcttgtt atcaacactt aatgggtttt gatgtagccg cgcacgcact
    56221 cttcgactaa gccccagaac cagtcggtat caatagtgat gccgactttg gtattagcgg
    56281 gcttgccaag aacgcccagc tcgtcgcaga cggtacgccc atagcaaggg ccactgttga
    56341 cgtccacttc gacgtacatc tcctgggttt taatgccatc agggttaatc agataaccaa
    56401 tgcaggtggc gtcgtgcacc gggccgccag ccaggccgta gttttcgaac tgggttttga
    56461 gagtgaagtt catgatgtcg ctgaacagct ctccggcagg gccgcctgcc ctttccattc
    56521 gtgcaatcac gtccggggtg caaacggtct ggttggtgag atcgaggccc atcatcacta
    56581 atggaacgcc agaggtgaac accacgcgtg cggcttccgg gtcagcaaag atgttgaact
    56641 cggcagatgg cgtgaagtta cctgtaccgt aagcgccgcc cattagcaca atttcacgga
    56701 ttttaggcag gatcgcgggt tgcatacgca ttgccaccgc gatatttgac aacggaccaa
    56761 ccggcaccag agtgatatcg ccatcgcttg ccatcagggt atcaatgatg tatttcaccg
    56821 cgtgggtact ttctgcctgg cgggtcagcg gctcgaatac cgggccatcc agtccggttt
    56881 caccgtgaat attatcggca acgatttgtt gacgcataat gggctgcggc atccccgcat
    56941 aaaccggaac attaatctcc agtttctggc aaacattcag gccattaatt aatgttttat
    57001 caagcgtctg attacccgca acaatggtga tacctaataa atcaatcgcc ggatgtttcg
    57061 ccgccatcat tatagcaata gcatcatcat gacccggatc acaatccaga ataatttttc
    57121 tcttttccat tgtttatttc ctctgtttcc agttgcgtta ttttttctac agcaaagaaa
    57181 tttcgatcgg gccgatgatt gaatcttaac aacagcgtac gtatgctaaa tatgagaaat
    57241 ctcatagcgg ataaacatcg tgaaagaaat ccacaataat gatcttaagc agcaattgat
    57301 gagtgaatcc gcgtttaagg attgcttttt aacggatgtt tcagccgata cgcggctgtt
    57361 tcatttttta gcgcgtgact acattgtgca ggaagggcaa cagccgtcct ggctgtttta
    57421 cctgacgcga ggccgcgcca ggctttacgc cacgctagct aatggtcgcg tatcgctgat
    57481 cgatttcttt gccgccccct gttttattgg cgagattgag ttaatcgata aagaccatga
    57541 accacgtgca gtacaggcga ttgaagagtg ttggtgcctt gcgctcccta tgaaacatta
    57601 ccgtccgctg ttattaaacg ataccctatt tttacgaaaa ctttgcgtca ccttaagtca
    57661 taaaaattat cgtaatattg tttctttaac tcagaatcaa tcatttccgt tagtgaatcg
    57721 cctggcagca tttatattac tctcgcagga aggtgatctt tatcacgaaa agcatacgca
    57781 agcggcagag tatttaggcg tttcttatcg acatctttta tatgttcttg cgcaattcat
    57841 tcacgacggt ttattaatta aaagcaagaa aggatatctc attaaaaaca gaaagcagtt
    57901 gtcagggctg gcgctggaga tggacccgga gaataaattc tccgggatga tgcagtaaaa
    57961 attatttgca atagcgcgat tgccggatgc aacgcttaat aagcgagtac atccggcaat
    58021 gaaactcaag ctaaaccaat aaagaacccg gcaatggtcg cgctcatcag gttggaaagc
    58081 gttgctgctg ccagcgcccg taaaccaagc tgggcgattt ccggagcacg ttttggcgaa
    58141 atagccgaaa atgccccaac gacaacaccg atagaaccaa agttagcaaa accacaaagc
    58201 gcaaaggaga taatcgcaat ggttttcact tccagcgtgc cgcccgtttg caggtatggg
    58261 gagaaactca ggtaagcgac gaattcgtta atcgccagtt tctgcccaat caggctaccc
    58321 gcaaggttgg catcactcca gtccacaccc atgatccacg ccagcggtgc cagcacatag
    58381 ccaaaaatac tttccagaga ggcattggca aaaccaaacc agccgccaat tccgccaata
    58441 atgccattaa tcagcgcaat aattgcgaca aacgccatta ccaccgtcgc cacaccggcg
    58501 gcgattttca gcccggtcat cgcaccgctc gccgctgctt cgataaagct ttttggcggc
    58561 gtttcgctga acgacagatt ttcaaacgtg acctgcgaaa gctcggttgc tggactaaga
    58621 atacgtgcaa acaaaatacc gccggggatc gccatcagcg atgccgctaa caggtagtca
    58681 attggtacgc ccattccggc ataaccaatc atcatcgaac cagcaatgga cgccatcccg
    58741 ctacaaatag ccgtaaacaa ctcgtttcga ttcatgcgat cgataaacgg tttaacgatc
    58801 gccgggatct cattttgccc gaggaaaata gtggtgactg cgacaaaaga ttcgattttg
    58861 ctgatgttga gggctttctg gaaaatgctg ccaaggatgc gaatcagcag ccccatcacg
    58921 ccaatgtagt acagcagact aatgagcgca gtgacgaaga taatcgccgg gagtacgcga
    58981 aaggcgaaga taaaacccgc accgtcaaag aggacatcca ttttcggccc gaccagcgaa
    59041 ccaaaaatga aggcgctacc ggcatcactg taagacatca ctttatgaac gcctaatgcc
    59101 gcctgttcta cagcccactt tcccggcgga aaatagagca tgatgccacc aatagcaatt
    59161 tgcagcagta gtgcggctcc aaccgtacgc aaactgatgc tctttttatt cactgacaac
    59221 agaaatgcta ttgccagtaa caccaccatc cccacaacac ttctcattat gtccataatg
    59281 attttccctt catgccagta aacccggcgt cagcgccagg ttttggtatg tttgatgagt
    59341 acgggcgacg gctttctgcc cgtcagaaat taacccgcga gacgctggta ttctttggca
    59401 atttcactcg ccagaatggc gttgttgaac accagttgga tgttggattt caggctgtca
    59461 ccgccggtca gttcagcaac gcgcgccagc aggaatggcg tactttcttt accaataaca
    59521 ccctgcgctt cagcttcagc taccgcctga tcgatcgccg cattgatagt gtgttccggc
    59581 atcgcaaact gttccgggat cgggttcgct accacgaggc caccgttcag cccgctttgc
    59641 catttcaccg ccattgcacg ggcaatttca ctggcgctgt cgagacgaat gctgacgtca
    59701 aacgggctgg tgcggcagaa aaacgcaggc agcgctttag tctgatagcc aattaacggc
    59761 acaccgaagg tttctaaata ctcagtggtt aatcctaaat cgagaataga tttcgccccg
    59821 gcacaaacaa cggtgacatt agtatttgcc agttcttgca aatcggcaga aatatcgaag
    59881 gtatgttccg ccccgcgatg aacaccacca attcccccgg tggcaaatac tttaattccg
    59941 gcaagcgccg caataatcat cgttgaagcc acagtggttg cgccattttt tccggcggca
    60001 acaacaaaag gtaaatcgcg acgactaact ttggtcacgt tatgcccttc acgacccagt
    60061 aattcaattt cttctttgct taaacccact ttcatcacgc cgccaataat ggcgatagtg
    60121 gcaggtactg cgccctgttt acgaatagtt tcttctactt caattgcggt ctgggcattt
    60181 tgtgggaacg gcatcccgtg agaaataatg gtcgattcca gcgccacaac cggttttttg
    60241 ttttttaaag cgtcctgcac ttccggggaa atttgtaata attcagggga aatttttaat
    60301 tcagacattc tgcgttctcc actaacgata taacgttggc aatcgataaa tcggggttat
    60361 tggtgtattc acaggagagc gccattgacg aatatccctg tgcgaaacga acagattcgg
    60421 caaacggcat tccgtctacc caacacgaag caagtcccgc catcatggca tcgcccgctc
    60481 cggtaacatt aataacattg gttttgatcg gcgcagacca accactttca ccgctgatat
    60541 cactgtaata aacgccgtcg ccgcccatgc tcaataccag tcggttcagg ccatgttgat
    60601 ggaaccaggc ggcaaccttt gccacatctt cacgccctga cagcgcaatc ccactcaggg
    60661 tttccgcttc aaggcggttt ggcttgagag tgtggatctg atttagacgg tcgcgcactt
    60721 tgacacattt ccacgctgaa accggatcga caaatacggg tacgttggca gcattatcca
    60781 gaatccatgc cagtgcctct tcactgatat tacagtccgc gacaatgacc tttgccctct
    60841 gaataaattc acggtgctgt gcgagatatt cagctgtaat agcgttgcta atattcatgt
    60901 catttatagc aaccagcatt tcaccggtat tatcgagtaa tgataaataa ctcgacgtat
    60961 tttctcccgg cacaatcagg catttatcga cataaacgcc agattgattg gtttgcgtta
    61021 gcagcgattg accataaaaa tcactaccta ctgcgctcag taaccaggct ttgttaccca
    61081 gcaacgccag gttttgtgca atatttcgcc ctactccacc aggtgtaaat tttattttac
    61141 ctggatttga atccgcataa tttaatgatt catgtgaata tccggcgaca tcaatattcg
    61201 ccgaacctat aattacgaca taatcctttt cgcgcatagc acgtcctctg gtaaaaaatt
    61261 accaaccctg tcaatccaca aggatataga tactgcaaag acggtttaaa catgtgttca
    61321 tatttaaaca tgtgctcata gtatgcatgt tttacgtaaa gtaaattgcc gccagagagg
    61381 caaagcgggc agatttgagc aggctcacaa aatgcgatta aatgcctgaa ttacaaaagc
    61441 gaggaatttg tgacgggatg cacagaacat gaacactaaa aaagggcaga aaagaagctg
    61501 cccttgagga aataacctta gtgtttgatg aacgtttcag gtttctactg aaagataaaa
    61561 ccatttaaat tcataaaatt acattcacaa accggtcatt ctctttgcgc tcatccaatg
    61621 atacagggca gtaaatttct gccctgtaac acacctttta ttacgcggct ttcgctactg
    61681 cgtccacttc cggacgtttc aggaaggcat aggccaaacc cgccaccagc gtaccggcaa
    61741 taattgctac caggtaaccc agtaccggcg taatagcgcc aggaatcagc agaacaaaca
    61801 gaccaccgtg cggtgccatc agtttcgcac caatcgccat tgagattgcg ccagtcagcg
    61861 ccccaccaac gatacaacac ggcagcacac gcatcggatc acgagcagca aacggaattg
    61921 caccttccga aataaagcac agaccaagaa ccagagcggc tttgccacct tcctgctgcg
    61981 ctttgtcaaa tttgcgacgc gccaccattg ttgccagacc cattgccagc ggtggcacca
    62041 tacctgccgc cataatcgcc gccatcgggc cataggtttg agtactcagc agacccacac
    62101 cgaatgcgta cgctgcttta tttaccggac cgcccatgtc agtacacatc atgccaccga
    62161 ggatcgcccc cagcagaacc gcattcgcag tccccatggt ctgcagccag tgagtcaacc
    62221 cttcgagaat gccagcaacc ggtttaccga tcaggtagat catcgccaga ccgaccacca
    62281 gactggaaat tagcggaatg atcaggatcg gtttcagcgc ctccatactc tgtggcagtt
    62341 tcagttgcgt actgattaac ttcgcaatgt aaccagccag gaagcccgca ataataccgc
    62401 caatgaagcc agaaccggtg ctgaccgcca gcataccgcc aatcagaccc ggcgtgagac
    62461 ccggacgatc ggcaatggag aaggcaatgt aacctgccag taccggcacc atcagcgcaa
    62521 aggctgaacc accaccaatc tgcatcagcg ccgctgccaa cgtacccggc tctttaaacg
    62581 cttcgatacc aaaagcaaaa gaaagcgcga tacacagacc acctgcaacg accatcggca
    62641 gcatgtaaga gacgcccgtc agcaagtgac ggtatgcgcc tgcactctct ttcttacctt
    62701 cagaggtcgc cgtttgagct ttgcccgccg gttcatacgg cgttgcttca gcaaccgctt
    62761 tatccagttc ctgcgcggtt ttcttcagcg ccagaccggt agaggtacga tacatcggtt
    62821 taccagcaaa tttcgccaga tccacttcga tatctgccgc cacaatcacc agatccgctg
    62881 cggctacttc ttcgggagtg attgcattac ccgcaccaac agaaccacgg gtttcaactt
    62941 tcacccacca gccacgtttt ttcgcttcgg tttcaatggc ttcagccgcc ataaaggtgt
    63001 gtgctacgcc agtcgggcaa gcagtcaccg caactacgcg tttcggaccg ctggcggcaa
    63061 ccggtgctgt cgcagcgacc ggcgcagtgt aaggtttcgc atggcctttg gcttcactca
    63121 ggaacagctc agggtgcgca actgcccggg aaatatcgcc cagccagaca tttttaccgt
    63181 tcagcgcgct gtcattcggg atggaatcac cgagaacaat cgccatttct gcgtcgttcg
    63241 gattgtcgat gatttccagt tttgcttttc gcgccgccgc gcccagcagg gtcttcgcca
    63301 tataggcgcg tgcctgaccg agattagcgt caataatcag cagcgttttc attatgcctc
    63361 tcctgctgtc agttaaaagg ttgtaagtcg acgcgcgcca tcattgcggc caactgcgga
    63421 cgatcggtaa tacccacatt gctttgactt accgccaggg ctgcaacagc agtcgccaga
    63481 cgcagtgtgt gttcactgga ttcacgcatt agcaagccat aaatcaggcc accaaccata
    63541 gaatcccctg cgccaacggt gcttacgaca tcgactgacg gtggtttggc gatccattcg
    63601 ccggaggcat taacccaaag cgcgccttcg gcacccagtg aaataacgac atgcgcaata
    63661 ccttgttcac gcagcgcatg cgcagcttca atcacatctt tcatttcagg cagtttacgg
    63721 cctgcccaga tttccagctc gcggcggtta ggtttcacca gccacggtgc cgctttcaaa
    63781 cctgctacta acgcttcacg gctactatca aagataatgc aagggcactg actacgcagg
    63841 cgagtcatcc agtcggtgaa cgcttccggg ctgacgcctg acggtaagct tccgctgaca
    63901 cagaccatat cgaactgacc gagccagctc agagaatcag tcacaaagcg ttcccagtcg
    63961 gcgggagtga cttcaaaacc cgagaagttg aagtcggtca cttcgccgtc tttttccgtc
    64021 agcttaacgt taattcgggt gcgcccctgt acaacctgga aacggttggc aatgcccagc
    64081 tcgctgaaca gttgctgaaa accatcctga ttgtctttac cgaggaagcc gccaacggtg
    64141 acatcaattc ccaggtcttt taatactttg gccacgttga tgcctttacc cgccgcatgc
    64201 agaccggtgg ttttcaccag gttcacttcg ccgcgttcaa tttccgggca gaaaccaaca
    64261 aggtcataag ccggattaag ggtgatagta gcaacacgtc tgctcattat gcgccctccc
    64321 caagaccagc agcgatagcg tcgccgattg ctttcagcgc ctgttcagca tctgcaccct
    64381 gggcggtaaa gcgtaggcga tgacctttct taacgccaag tgccacaact ttcatcagac
    64441 tacgtccgtt tgccggtttg ccggtgccat caaggtttgt cacggtaata tcactattaa
    64501 attgtttaat ggtgttgacc agcatggtac ctggacgagc atgcaggccg tgttcattgc
    64561 gcaccacaaa ctccgcgctt aacacgtcgt cggtcggcgc atcatcgctg gtcagcagcg
    64621 ccagcaacgt tgccgcatcc gctttcagca agcggtcagc tttattgtcg agcaacaaat
    64681 cagcgagacg cttaagaacc gcaatgggct gatcgtcatt catcgccaca ctcaccagca
    64741 tggctgccgt ttcgccgtcc acatcaaaag catttgccgc acggcttacc gcaatcgcgc
    64801 tacgcaaatt gccttcggcg ctatcgctca gccagatgcc ctgtccgagg ttcagcggtt
    64861 gttcattgat ggctttggtg acgaaagtgg cgtcaactgc ccccgcttct ttcagacgcg
    64921 cagcgttcag cgcctgaaga gtcagcagat cgctggcgac gatatccagc gtcagcattt
    64981 cgttgtcgag cttcagctgc tcactctgct tttcgcccat cagcaatgcg cgaagttctt
    65041 ctgctgttgt tgctgacttc agttgttcag caacggaatc atcgctcagt acgtgggtca
    65101 gctgacgtag caggcccaga tgctcatccg agctggcagc aataccgatc gctacgtacg
    65161 ctacctgacc gtcaccccaa gtgacgcctt ccgggaactg aaatacctga acgccggttt
    65221 tcagcacctg atcgcgggtg tcggtagtgc cgtgtggaat agcaatacca ttgccgagga
    65281 acgttgaggt ttgctgttcg cgcgccagca tgccattgac gtagccttct gctacattac
    65341 cggcctgcac cagcgccgca gcgacctggc gaatcgcctc ttctttgtct ccggcctttt
    65401 cgcccggatg gatgtcctgt acggataact ggaacatagt tctcctctct tgctgaattg
    65461 aaacgattca gcttctatga gaaaaaaagc gccaacctgg cttagggtta aagacaagat
    65521 cgcgctgaaa cgtttcaaga aagcataata cttctgtttc agcacgcaag gaaagccgta
    65581 aagttatgag caaaagtttg atgtgctgca caattttttg tcatttttcc taattgctga
    65641 tgggaaaatc cgttatcagc aatttcattt cagcagcgtc agcagccaat cagcaacagt
    65701 gaaatggcat tttgattatt cgtggcgctt ttgatttcgc tgtctgttta ttttctgaca
    65761 agcagcgtaa actccgcgtc ttcctcttcc agtgatcgac cagcatgcat aactcccccg
    65821 cagtctccag cgcgaaatcg tttgacctga cctcgacggc gtttttaatc gttgcctttc
    65881 tcaccggtat tgcgggcgct ctgcaaaccc cgacactcag tatttttctt accgatgtag
    65941 tacatgcccg tccggcgatg gtgggattct tctttaccgg cagcgctgtc attgggattc
    66001 tggtgagtca gtttctcgcc gggcgctctg ataagcgcgg cgatcgcaaa tcgctgattg
    66061 tcttttgctg cctgttaggc gtgctggcct gcaccctttt tgcctggaat cgcaactact
    66121 ttgttttgct attcgttggc gtctttctta gcagctttgg ctcgaccgct aacccgcaaa
    66181 tgtttgccct tgcccgtgaa catgccgaca aaaccggacg tgaagcggtg atgttcagct
    66241 cttttttacg cgctcaggtt tcactggcat gggtcattgg cccaccgctg gcttatgcct
    66301 tagcgatggg tttcagcttt acggtaatgt atctgagcgc agcggtagca tttattgttt
    66361 gcggcgtgat ggtgtggctg tttttaccgt cgatgcaaaa agagcttccg ctggcgaccg
    66421 gcacggttga agcgccgcgc cgtaaccgtc gcgatacgct gctgctgttt gtcatttgta
    66481 cattgatgtg gggctcgaac agcctgtaca tcatcaacat gccgctattt attatcaacg
    66541 aactccatct tcccgagaaa ctggccggtg tgatgatggg gaccgccgcc gggctggaaa
    66601 tcccgaccat gttgattgcc ggatatttcg ccaaacgtct gggtaagcgt ttcttaatgc
    66661 gcgttgctgc cgtgggtggc gtctgttttt acgcaggaat gctgatggcg cattcacctg
    66721 ccattctgtt gggcttgcag ctgctaaatg ctatttttat tggcattctg ggcggtatcg
    66781 ggatgctcta ttttcaggat ttgatgcccg gtcaggcagg ttcagccacc acgctctata
    66841 ccaacacgtc gcgcgtgggc tggatcatcg caggatcagt ggcgggcatc gtcgccgaga
    66901 tctggaatta tcacgctgtg ttctggtttg cgatggtgat gattatcgcc actctgtttt
    66961 gcttactgcg gattaaagat gtttaaggcg cggtcagcat ttccagatcg ataagccagg
    67021 tcattgcctg ctgacgcgag ttaccgcaca tttcagcgct ggcttgtaag cctgcacaga
    67081 ctttcggccg cagtggcgag gtgaaaattt tacagcgttg ctgttcatca agctgaatgc
    67141 agggcgtatt ggcgggcttg ccatctggca taccgggaat agggctggaa atcgaagggg
    67201 cagtgcaaca cgccccgcaa cccggacggc attccatacg ttgttctctt aaggttaacc
    67261 accggattga tgagcgcgca cagtaccacc ttttttgcac tagcaaaagt gcgaatacca
    67321 cttgcccgaa aggcccgtcg cgagtacttt gtcgcgatat ttttgacatt ttcgactaca
    67381 ggaatttttc gatgccaaga gcgaacgaaa ttaaaaaagg tatggtactg aattacaacg
    67441 gcaaactgct gctggttaag gatattgata ttcagtcgcc cactgcccgc ggtgccgcta
    67501 cgctgtacaa aatgcgtttt tctgatgtcc gtaccgggct gaaagtagaa gagcgtttca
    67561 aaggtgatga tatcgttgac accgtgacgc tgacccgccg ttacgttgac ttctcctatg
    67621 tcgatggtaa cgaatatgtc tttatggata aagaagacta taccccgtat accttcacca
    67681 aagatcagat tgaagaagag ttgctgttta tgccagaggg cggcatgccg gacatgcagg
    67741 tgctgacctg ggacggtcaa ctgctggcgc ttgagctgcc gcagaccgtt gatctggaaa
    67801 tcgttgaaac cgcaccaggc atcaaagggg catctgccag cgcccgtaac aaaccggcga
    67861 cattgagcac tggtctggtg attcaggtac cagaatattt aagcccgggc gaaaaaattc
    67921 gtatccatat cgaagaacgc cgttatatgg ggcgtgctga ctaacttcag ccgcatgcag
    67981 aaaagggata gctcaggctg tccctttttt aatttattat accaatcttc tattttgcgc
    68041 ttcctgaaca ccttacgccc gcaatattca ctcgttgatt gatcgccctc acacttcatc
    68101 gcattaacaa tccagaccaa tttcaattgc tgtcatataa ctttacactg tcattgttaa
    68161 ttaatcgtta ctaagacgtg actcctatga atacaatcgc ctccgttacg ctcccgcatc
    68221 atgtacacgc tccacgctac gatcgccagc agttgcaatc acgtatcgtt cattttggct
    68281 ttggagcctt tcaccgcgca catcaggcgt tactgaccga tcgtgtgctg aatgcccagg
    68341 gcggcgactg ggggatctgt gaaatcagct tgttcagcgg tgatcaactg atgagccagc
    68401 tccgcgcaca gaaccattta tataccgtgc tggagaaagg tgcggacggc aatcaggcga
    68461 taatcgtcgg tgccgttcac gaatgcctga atgcaaaact ggattcctta gcggcaatta
    68521 ttgagaaatt ttgcgagcca caggtggcaa ttgtttccct gacgattacc gaaaaaggct
    68581 attgtattga cccggccacc ggtgcactcg acaccagcaa tccgcggatt attcacgatc
    68641 tacaaacccc tgaagaacct cactccgcgc caggtattct cgtcgaagca ctgaaacgcc
    68701 gccgtgagcg cggccttaca ccgtttaccg tgctctcctg cgacaatatt cccgacaatg
    68761 gccatgtggt gaaaaacgcg gtgctgggaa tggcagaaaa acgttcgcca gaactcgccg
    68821 ggtggataaa agaacacgtc agttttccgg gaaccatggt cgaccgcatt gttccggctg
    68881 caaccgccga atcactggca gaaatcagcc agcatctggg ggtaaatgat ccctgcgcga
    68941 ttagctgcga accgtttatc cagtgggtgg tggaagataa cttcgtcgct gggcgtcctg
    69001 cctgggaagt cgcaggtgta caaatggtga atgatgtcct gccatgggaa gagatgaaac
    69061 tgcgtatgct taatggcagc cactcttttc tcgcttatct gggttacctc tcaggattcg
    69121 cccatatcag tgattgcatg caggatcgcg catttcgcca tgccgccaga acattaatgc
    69181 tggatgagca agcgccgaca ctgcggatta aagatgtcga tttaacacaa tatgcggata
    69241 agttaattgc acgttttgct aatccggcgc tgaaacataa gacctggcaa atcgcgatgg
    69301 atggcagcca gaaattaccg caacgcatgc tggcaggtat tcgcatacat ctggggcgtg
    69361 aaacggactg gtcgttgctg gcattaggcg ttgctggctg gatgcgttac gtcagcggcg
    69421 ttgatgatgc cggaaatgcc attgatgttc gcgatccgct tagcgataaa attcgcgaac
    69481 ttgttgcggg cagcagcagt gaacaacgcg tcaccgccct gctttccctg cgtgaagttt
    69541 tcggtgatga tctgccagat aacccgcatt ttgtgcaggc catcgaacaa gcctggcaac
    69601 aaatcgtaca attcggcgca catcaggcgc tattaaacac cctcaaaatt taacgatttc
    69661 tgcggttaaa gcggatgaag ctcgccttcg tccgctctcc ccttctcttt tctgccgttt
    69721 ttagccagga ttaacgctca gttaacttat cagagtattg cggagccgtt gtgaccagga
    69781 ccaacctcat caccggtttt ctcggcagcg ggaaaactac gtcgattctt catctgttag
    69841 cccataaaga tcccaacgaa aaatgggcgg tactggttaa tgaatttggg gaagtcggaa
    69901 ttgatggtgc tttgctcgcc gatagcggcg cattgctgaa agagatcccc ggcggctgca
    69961 tgtgctgcgt taatggttta cccatgcagg tagggttgaa taccttactg cgtcagggaa
    70021 aaccagaccg cttgttgata gagccgactg ggctgggcca tccgaaacag atcctcgatc
    70081 tgttaaccgc accagtctat gaaccgtgga tagatctgcg cgccacgttg tgcattctcg
    70141 atccacgcct gctgctggac gaaaaaagcg ccagcaatga aaacttccgt gaccagctgg
    70201 ctgccgcaga catcattgtc gccaataaat ccgaccgtgc gacacctgaa agcgagcaag
    70261 cgctacagcg ctggtggcag caaaatggtg gcgatcggca attaattcac agtgagcatg
    70321 ggaaagttgc cggtcatctt ctggatttgc cgcgtcacaa tttagccgag ttgcccgcca
    70381 gcgccgcgca ttctcatcag catgtcgtga aaaaagggtt agcagcgtta agcctgccag
    70441 agcatcaacg ctggcgtcgc agtctgaaca gtggacaagg acatcaggcc tgcggctgga
    70501 tattcgacgc tgatacggta ttcgacacca ttggcattct ggaatgggcg cgacttgcac
    70561 cggtggaacg cgtcaaaggc gtgctgcgta ttcccgaagg gctggtgcga atcaaccgtc
    70621 agggcgatga cctgcacatt gaaacgcaaa acgttgcgcc accggacagc cgtattgagc
    70681 tgatttccag cagcgaagct gactggaatg ccttgcagag cgcgctgttg aagcttcgtt
    70741 tagcgactac cgcgtaaggt tgcctgcgtt tttcagtaag ataattagag aaaatatgat
    70801 taaaaatttg ccgcaaatag tgttgttgaa tattgtcggc ctcgcgctgt ttctttcctg
    70861 gtatattccc gttaatcatg gattctggtt gccgattgat gcggatattt tttatttctt
    70921 taatcagaaa ctggtcgaaa gtaaggcctt tttgtggctg gttgcattga ccaacaatcg
    70981 cgctttcgac ggttgttcac tgctggcgat gggaattttg atgctgagtt tctggctgaa
    71041 agaaaacgcc ccaggcagac gacgtatcgt gattattggt ctggtcatgc tattaactgc
    71101 agtggtatta aaccagctgg gtcaggcatt aattcctgta aaacgggcca gcccaacatt
    71161 gacttttacc gatattaacc gcgtcagcga actgctctct gtccccacga aagatgcctc
    71221 acgagatagc tttcccggcg atcacggcat gatgctgctt attttttcgg cattcatgtg
    71281 gcgttatttc ggcaaagttg caggtcttat cgcccttatt atttttgtgg tttttgcgtt
    71341 tcccagagta atgattggcg cacactggtt tactgacatc attgtcggtt cgatgaccgt
    71401 gatattgatc ggtttgccct gggtgttgct gacgccatta agtgatcgat taatcacctt
    71461 ttttgacaaa tcactaccgg gaaaaaacaa acatttccaa aacaaataac tcacagtaat
    71521 taacatcatc aggtttattt ttatagtgag gataatcctg atgatgcgca ccgtgctttc
    71581 atccatcgaa agcaaaaatc attctctaag taaatgaatg gattgcatgc atttcactca
    71641 atcgtacttt aattgaccaa tcccgcttat taactttctg tatcactttt tcttataaaa
    71701 aatcatgtaa aaccgctcgc caagaccgca ccaatcgggt aatctcgaac tcgttttgcc
    71761 tcggcggtag attatcctca cagcatataa ttttgtgcgt tagtccacag atttggcctt
    71821 aaggaattgt ttcaacatgc ccaggtaatt agtctcgtgt cgcttggcat ttttttataa
    71881 cgatatttgt cgttaaggac ttcaagggaa aacaaacaac atggtcaaat ctcaaccgat
    71941 tttgagatat atcttgcgcg ggatccccgc gattgcagta gcggttctgc tttctgcatg
    72001 tagtgcaaat aacaccgcaa agaatatgca tcctgagaca cgtgcagtgg gtagtgaaac
    72061 atcatcactg caagcttctc aggatgaatt tgaaaacctg gttcgtaatg tcgacgtaaa
    72121 atcgcgaatt atggatcagt atgctgactg gaaaggcgta cgttatcgtc tgggcggcag
    72181 cactaaaaaa ggtatcgatt gttctggttt cgtacagcgt acattccgtg agcaatttgg
    72241 cttagaactt ccgcgttcga cttacgaaca gcaggaaatg ggtaaatctg tttcccgcag
    72301 taatttgcgt acgggtgatt tagttctgtt ccgtgccggt tcaacgggac gccatgtcgg
    72361 tatttatatc ggcaacaatc agtttgtcca tgcttccacc agcagtggtg ttattatttc
    72421 cagcatgaat gaaccgtact ggaagaagcg ttacaacgaa gcacgccggg ttctcagccg
    72481 cagctaataa accgtttgga tgcaatccct tggctatcct gacgagttaa ctgaaagcac
    72541 tgcttaggca gtgctttttt gttttcattc atcagagaaa atgatgtttc cgcgtcttga
    72601 tccaggctat agtccggtca ttgttatctt ttaaatgttg tcgtaatttc aggaaattaa
    72661 cggaatcatg ttcatacgcg ctcccaattc tggacgtaag ctcctgctta cctgcattgt
    72721 tgcaggcgtg atgattgcga tactggtgag ctgccttcag tttttggtgg cctggcataa
    72781 gcacgaagtc aaatacgaca cactgattac cgacgtacaa aagtatctcg atacctattt
    72841 tgccgacctg aaatccacta ctgaccggct tcagccgctg accttagata cctgccagca
    72901 ggctaacccc gaactgaccg cccgcgcggc gtttagcatg aatgtccgta cgtttgtgct
    72961 ggtgaaagat aaaaaaacat tctgttcatc tgcgaccggt gagatggaca ttccactcaa
    73021 tgaattgatt ccggcgctcg acattaataa aaatgtcgat atggcgatct tacccggcac
    73081 gccgatggtg ccgaacaaac ccgcaatcgt catctggtat cgcaaccctt tgctgaaaaa
    73141 tagcggcgtc tttgccgctc tgaatctcaa cctgacgcct tcactctttt atagttcacg
    73201 gcaggaagat tacgatggcg tcgccctcat tattggcaat actgcgctat ctaccttttc
    73261 ttcacgtttg atgaacgtta acgaattaac cgacatgcca gtccgtgaaa ctaaaattgc
    73321 gggcattcct ctgaccgttc ggctttatgc agatgactgg acatggaacg atgtgtggta
    73381 cgcattttta ctgggcggca tgagtggaac tgtcgttggc ctgctctgct attacctgat
    73441 gagcgtacgt atgcgccccg gcagagaaat catgaccgcc atcaagcgcg aacaatttta
    73501 cgtggcgtat caaccggtgg tggatacaca agctttgcga gtaacgggcc tggaagtact
    73561 gctacgctgg cggcatcctg tcgcgggaga aattcccccg gatgccttca ttaactttgc
    73621 cgaatcgcaa aagatgattg tgccgctgac tcagcacctg tttgagttaa ttgcccgcga
    73681 tgccgcagaa ttagaaaaag ttctgccggt aggcgtcaaa tttggcatta acattgcgcc
    73741 ggaccatctg cacagcgaaa gctttaaagc agatatccag aaactgctca cttccctacc
    73801 cgcacaccat ttccagattg tgctggaaat taccgagcgc gatatgctga aagagcaaga
    73861 agccacacaa ctcttcgcct ggctgcactc ggtcggcgta gaaattgcta ttgatgactt
    73921 cggcaccggg cacagcgcgc ttatctatct tgagcgtttt acgctcgatt atctgaaaat
    73981 tgaccgtgga tttatcaacg ccatcggtac ggaaacgatc acttcacccg tacttgacgc
    74041 ggtgctgacg ctggcgaaac gcctcaatat gctgacggtt gctgaagggg tcgaaacgcc
    74101 ggaacaggcg cgatggctaa gcgaacgcgg cgttaatttc atgcaaggct actggattag
    74161 tcgcccgtta ccgctggacg attttgttcg ctggctgaag aaaccgtata cgccgcagtg
    74221 gtaaggtgtg cttacgtccc ttattattca tagtgaaagc atgccggatt gcggctaatg
    74281 atgagtaaaa ggaaatccgt tgcagatgat tgtgcgcata ctgctgctgt ttatcgctct
    74341 gttcaccttt ggtgcgcagg cgcaggctat caaggaaagc tatgcctttg ccgtgctggg
    74401 cgaaccccgg tacgcgttta atttcaacca ttttgattat gtgaaccccg ccgcgccaaa
    74461 aggtgggcag ataacgttgt cagccctcgg caccttcgat aatttcaacc gctatgcact
    74521 gcgcggcaac ccgggcgcac gcaccgagca gttgtacgac acgctattta cgacttccga
    74581 tgacgaacca ggcagttatt acccgctgat tgctgaaagt gcacgctatg ctgacgatta
    74641 ttcctgggtg gaggtcgcta ttaatccgcg cgcccgtttt catgatggtt cgcccattac
    74701 tgcccgcgat gtagagttta cttttcaaaa atttatgacc gaaggcgtgc cgcaatttcg
    74761 tctggtctac aaaggcacca ccgtcaaagc cattgcgccg ttaaccgtgc gcattgagtt
    74821 agctaaaccc ggcaaagaag atatgttgag tctgttttcg ctgccggtat ttccagaaaa
    74881 gtactggaag gatcacaaac ttagcgatcc gctcgccacg cctccgcttg ccagtggtcc
    74941 gtaccgaatt acgtcctgga aaatggggca aaatattgtc tattcccgcg tgaaagatta
    75001 ctgggcagca aacttaccgg taaaccgtgg acgctggaat ttcgacacca ttcgctacga
    75061 ttattacctc gatgataatg tcgcctttga agcgtttaaa gcaggtgctt ttgatttgcg
    75121 tatggaaaac gacgctaaaa actgggccac gcgttatacc ggtaaaaatt tcgataaaaa
    75181 atacatcatc aaagatgagc aaaagaacga atcagcccag gatacgcgct ggctggcgtt
    75241 taatatccaa cgtccggtat tcagcgatcg ccgggtccgg gaagcaatca ccctcgcctt
    75301 tgactttgaa tggatgaaca aggcgttgtt ttacaatgcc tggagtcgca cgaacagtta
    75361 ttttcagaat accgaatacg cggccagaaa ttaccccgac gccgcggagc tggtgcttct
    75421 ggcaccaatg aaaaaagatc taccgtcaga agtcttcaca caaatctacc agccgccggt
    75481 atctaaaggc gatggctacg atcgtgacaa cctgttaaaa gccgacaaac ttcttaacga
    75541 agcaggctgg gtgctgaaag gtcagcaacg cgttaatgcc actacgggtc agccgctcag
    75601 ctttgaatta ttgcttcctg caagcagcaa tagtcagtgg gtattgccgt tccagcacag
    75661 cctgcaacgg ctgggtatca acatggacat tcgcaaggtg gataactcgc aaatcaccaa
    75721 ccgcatgcgc agtcgcgact atgacatgat gccgcgcgta tggcgggcga tgccgtggcc
    75781 cagttccgat ttacagattt cctggtcatc ggaatatatc aattccactt ataatgcccc
    75841 cggcgtgcaa agcccggtta tcgactcgct gatcaaccaa attattgccg cgcagggaaa
    75901 taaagaaaaa ttactgccgt tggggcgagc actggatcgc gtattaacgt ggaattatta
    75961 catgctgcca atgtggtaca tggcggaaga ccgtctcgcc tggtgggata aattctccca
    76021 acccgctgta cgccctgttt acagcctggg tatcgatacc tggtggtatg acgttaataa
    76081 agcggccaaa ctgccgtcag ccagacaaca gggagagtag atgggcgctt atctgattcg
    76141 ccgtctgttg ctggtgatcc caacgttatg ggcgattatc actatcaact ttttcatcgt
    76201 gcaaattgcg cctggcggtc cggtcgacca ggccatcgcc gccattgagt ttggtaatgc
    76261 cggagtatta cccggcgcag gcggtgaagg tgttcgtgcc agccatgcgc aaacgggtgt
    76321 cggcaatatc agcgacagta attaccgtgg cggacgcgga ttagatccag aagtgatcgc
    76381 cgagatcact catcgctacg gtttcgataa gccgatccat gaacgttact tcaaaatgct
    76441 ctgggactac atccgctttg attttggcga tagcctgttt cgcagcgcct cggtgctgac
    76501 gctgattaaa gacagtctgc cggtttccat caccctcgga ttgtggagca cgctgattat
    76561 ctatctggtg tcgattccgt taggcattcg caaagctgtt tataatggga gccgctttga
    76621 cgtctggagt agcgcattta tcatcatcgg ctacgccatt ccggcctttt tgtttgccat
    76681 cctgctgatt gtcttcttcg caggcggcag ctatttcgac ctgttccctc tacgcggcct
    76741 ggtttccgct aactttgatt cgctgccgtg gtatcagaaa atcaccgatt atctgtggca
    76801 tatcacgctg ccggtgctgg cgacggtgat tggtggcttt gcggcgctga ctatgctgac
    76861 aaaaaactca ttccttgatg aagtacgcaa gcaatacgtg gtgaccgcgc gtgcgaaagg
    76921 ggtaagtgaa aaaaatattc tctggaaaca tgtgttccgc aacgccatgc tgctggtgat
    76981 tgccggtttt ccggcgacgt ttatcagcat gttttttacc ggctcgctgc tgattgaggt
    77041 gatgttttcg ctcaatggtc ttggcttact gggctacgaa gcgaccgtct cgcgcgatta
    77101 tccggtgatg tttggtacct tgtatatttt caccctgatt ggcctgctgc tgaatattgt
    77161 cagtgatatc agctatacgc tggtcgatcc gcgtatagat tttgagggac gttaatgtcg
    77221 cgactcagcc ccgtcaatca ggcccgttgg gcgcgttttc gtcataaccg tcgcggctac
    77281 tggtcgttat ggattttcct cgtcttgttt ggtttgagtt tgtgttctga actgatcgcc
    77341 aacgataaac cgttgctggt gcgttatgac ggtagttggt atttcccgtt actgaaaaac
    77401 tacagtgaaa gcgattttgg cggcccgctg gcaagtcagg ctgattatca ggacccgtgg
    77461 ctgaaacaac gactgggaga taaaggctgg gtactgtggg cgccgattcg ctttggcgct
    77521 accagtatca acttttccac cgacaagccc ttcccctccc cgccttcccg gcaaaactgg
    77581 ctgggaacgg atgccaacgg cggcgatgtg ctggcgcgca ttctgtatgg cacgcggatc
    77641 tctgtcctgt ttggtctgat gttgaccctc tgctccagta taatgggcgt actggcgggg
    77701 gcgctacaag gctattacgg cggtaaagtc gatctctggg ggcaacgctt tattgaagta
    77761 tggtcgggga tgccgacact gtttttgatt attttacttt ccagcgttgt gcaacctaac
    77821 ttctggtggc tgctggcaat tactgtctta tttggctgga tgagtctggt cggcgtggtg
    77881 cgggcggagt ttttacgtac ccgcaatttc gactacatcc gcgcggcgca ggcgcttggc
    77941 gtcagcgatc gcagtattat tctgcgtcat atgttgccga atgcaatggt cgctacccta
    78001 acctttttac cgtttatttt atgtagttcg ataaccaccc tgacctcgct cgatttcctc
    78061 ggtttcgggt tgccgctcgg ttcaccgtcg ctcggcgaac tgctattaca agggaaaaat
    78121 aaccttcagg ccccgtggct tgggatcacc gccttcctgt cggtggcaat attattgtct
    78181 ttgctgatct ttattggtga agccgtccgc gacgcatttg atcctaataa ggcggtgtag
    78241 catgacgcaa actctgttag cgattgaaaa tttgtcggtg ggttttcgcc atcagcaaac
    78301 cgtacgtaca gtagtcaatg atgtttcact acagattgag gctggcgaaa cgctggcgct
    78361 ggtgggtgag tcaggttcag gcaaaagcgt taccgcgctg tcaattttac gcctgctccc
    78421 atccccgccg gttgaatatc tctccggcga tattcgtttt catggcgaat cgctgcttca
    78481 tgccagcgat caaacgttgc gcggtgtacg cggtaataag atcgccatga tttttcagga
    78541 gccgatggtg tcgttaaatc cattgcatac cctggaaaaa cagctttatg aagtgctttc
    78601 actccaccgc gggatgcgtc gggaagcggc tcgtggcgaa attcttaact gccttgatcg
    78661 cgtcggtatc cgccaggcgg caaaacggct gacagattat ccgcatcagc tctccggcgg
    78721 cgaacgccag cgggtgatga ttgcgatggc actgttaacg cgaccggaat tattaattgc
    78781 cgatgaaccg accaccgcac tggacgtctc tgtccaggcg cagattttac agctgttgcg
    78841 cgaactgcaa ggcgagctga atatgggcat gctgtttatt actcataacc tcagcattgt
    78901 cagaaaactg gcccaccgcg tggcggtaat gcaaaacggt cgctgtgtcg agcaaaataa
    78961 cgccgctacg ctatttgcct cacccactca tccttacaca caaaagctac tcaacagtga
    79021 accgtcaggc gacccggtgc cattgccaga acctgcctca acgttgctgg atgttgaaca
    79081 gcttcaggtt gccttcccca ttcgcaaagg gattttgaag cgcattgtgg atcataatgt
    79141 ggtggtgaaa aacatcagtt ttacgctacg agcgggtgaa acactgggtt tagtgggcga
    79201 gtccggttcc gggaaaagca cgacgggact ggcgctgctg cgactgatta attctcaggg
    79261 cagcatcatc tttgacggtc agccactgca aaatttaaat cgccgccagc tgttacctat
    79321 tcgtcatcgc attcaggtgg tatttcagga tccaaactcc tcgctcaacc cacgactcaa
    79381 cgttttgcag attattgagg aaggcttacg ggttcaccag ccgacgcttt ctgccgcaca
    79441 acgcgaacaa caagtgatag ccgtgatgca tgaagtggga ttagatcctg aaacacgcca
    79501 ccgttatccg gcggagttct ctggtggtca gcgacaacgt attgcgattg ccagggcatt
    79561 aattcttaag ccctcgctga tcatacttga tgaaccgaca tcatcactcg acaaaacggt
    79621 acaggcgcaa atattgacgc tattgaaatc attgcaacaa aagcatcaac tggcctattt
    79681 gtttatcagc catgatttgc acgttgtccg cgcgttatgt catcaggtta tcgtactgcg
    79741 acaaggggaa gtagtggaac aaggaccgtg cgcgcgcgtg tttgccacac cgcagcagga
    79801 gtatacgcgt cagctactgg cgttgagctg acgcttaaaa aggattgtag tttgaaaagg
    79861 gttcggcaat tgccacacca aaatttttca atcggcatgt tgcagcaaac tcgtcctggc
    79921 gatttacaaa caggcacggc tcaccttcac actccagcac cgaacatgga acttcgatgt
    79981 cgcttaacgc ctggcttagt ttatacatca ccgaccatgc attatcacca tccgggctaa
    80041 gcaatttaag cgccaccagg ctgttgtcac cgcacggtcc attttgcgga atcggttcaa
    80101 ccttcgaacc tgcgaaacca gcagaccagc gatagttctg cggcagtcga tggacgattg
    80161 agagttgtaa tgaagtcact tattttcccc ggaagcacat ttacttcaca atttatttac
    80221 atcaatttta acacatcatc aacaatccgt ctttatacag atcgtaaaga ttgatgcctc
    80281 gcctgcgtgg cctctatggc tctgatttaa gtaatatctg cgggcgtggt cacgtttgcg
    80341 gggctatatg tacccgtttc tgcgatctaa ctcaaccttt ttaactacaa tgatgtgact
    80401 ttttacataa attgatttta cataaaataa acatatatcg ggggaatgat agatttgtgg
    80461 ttgacatgca tcaacaaagg aagcctttta gcttcctcgt tgtgcaatag atcaccgttt
    80521 tttcggccga ctggcgtaca gacagaagag aatggagctg gttgcgcaga atgcaattga
    80581 ccaaatcatc ggccatgcag agttaaaggt cgcaagagaa agcaatgcgc caacaattgc
    80641 ccctatgcca aaacggaagg ttcctgccag cgaagatgcc gttcccgcca tatggggaaa
    80701 ctcatcaaga atgaccgcca tcgcattgga tgacaccatc gacacgcagc ccacaaacgc
    80761 cgcaacgcca accaccagcg accagaatcc cagccccagt agcgcactga tgaccatcca
    80821 cgctgccata ataaactgta tccacaaccc cgagcggaac atatttaacg cgccaatacg
    80881 gcggacgaag cggctattaa agatggtcat cacgaacagg aaaacaatgt ttagtgcaaa
    80941 gtaataacca aagttttccg gcgcgacgtg gttaatttca atgtagacaa acggtccggc
    81001 gcttaagaac gagaacatcc cggcaaagct gaaaccactg gccagcatat aactcaggac
    81061 acgcttatga cggaacagcg ccgcaaagtt accaatagtg gtacgaatgt gaaaaggctg
    81121 gcgacgctcc ggtggtaagg tttctttgat cagaaagaag atcattgccg aagccagaat
    81181 cgccgccacg gcgaggatcc agaagatgta atgccagctc agccacacca gcacccagcc
    81241 gccaactatc ggtgccatca gcggtgcaat ggttgtcacc agcatgacaa acgacatcat
    81301 ccgcgagaac tcttctttag ggtagatatc acgcatcaaa gcgttgataa ccacgctggc
    81361 cgcagccgcc gccagcccgt ggaagaaacg catcacaatc agctgatcga tggtttgtgc
    81421 caacgcacac gccaccgcgg cggcggcaaa caccagcgta ccgccaagaa ccaccggctt
    81481 acgcccgaag ctgtctgcca tcggcccgta gattaactgc cccaacgcaa agcccagaat
    81541 ataagtactg agggtcatct gcgtactgcc cgccggtacg ccaaactgcg ctgaaattac
    81601 cggtagcgcg ggcagataca tatcaatcga cagcggcatc aacatggcca gcaggccaag
    81661 gataaaaaca atagcaaacg acgaatgctg tcgggtggtc acaacgggct cctgaaagtc
    81721 attgaaaagt tagacgacgc tggcaatttc ttcttcagtt aacggacgat attcaccggg
    81781 ggctaaatca gcatccagcg taatgccgcc aatacgttca cgatgcagct caaccacatg
    81841 gttacccaca gcggcgaaca tgcgtttcac ctgatgataa cgcccttcgc tgatggtcag
    81901 acgaacctgc gttggggtaa tcacttccag caccgccggc ttagtgagat ctttttcgtt
    81961 atgcagctgc acgcctttag caaattgctc tgccgtatcg tcagctacgg gtgattccag
    82021 cgtcaccaga taggtcttct cgcaatgatg gcgcggagaa gtaatgcggt gcgaccactg
    82081 accatcatct gtcattagta ccagaccggt ggtatcgata tccagccgcc ctgcggcgtg
    82141 cagtttccac gccactggtt catctagaaa atagagcacc gttgggtgat cagggtcgtc
    82201 cgtggagcaa acatagccct gaggcttatt gagcatgaag taacgtggac cgtgttgctg
    82261 cgccagcggg ttgccatcgt aagcgacatc atgttcagga agcagtttga acgctgcatt
    82321 acggacgatt tcgccatcga cggtgacacg attgccgcgg atttcacgcc cggcaatagc
    82381 acggctaacg ccgagttgct gtgcgataaa tttatcaagt cgcatttgtg tgattttgcc
    82441 tgtaaaaacg gaggtcgggc attgacccga aaatctgaac tgttgtctgc ccagtatagc
    82501 ggtctaataa cttccctcaa gggaaaaaga accgtggcat actattagca gaataatcta
    82561 cctacgcgag accatgattt ttacacttcg cccatatcag caagaagccg tggatgccac
    82621 gctcaatcat tttcgtcatc ataaaacccc tgccgttatc gtgctgccca ccggtgcagg
    82681 taaaagcctg gtgatagcgg aactggcgcg gctggctcgt ggtcgcgtgc tggtgctggc
    82741 acacgttaaa gaactggtgg cgcaaaacca tgcaaaatat caggcgctgg gactggaagc
    82801 cgatattttt gccgccgggc taaagcgcaa agagagccac ggtaaagtgg tatttggcag
    82861 cgtgcagtcg gtcgcccgta atcttgatgc ctttcagggt gaattttcgc tgttgattgt
    82921 cgatgaatgt caccgtattg gtgacgatga agagagccag tatcagcaaa tcctcactca
    82981 cctgacaaaa gtgaatcccc acttacgcct gctggggctg actgccacgc cttttcgact
    83041 gggcaaaggc tggatctacc agttccatta tcacggcatg gtacgcggcg atgagaaagc
    83101 ccttttccgt gactgcattt atgaactgcc gctgcgttat atgattaaac acggctatct
    83161 gacgccgcca gaacgactgg atatgccagt agtgcaatac gatttcagcc gcttgcaggc
    83221 acagagtaat gggctgttca gcgaagccga tctcaaccgt gagctgaaaa aacaacaacg
    83281 tattaccccg cacatcatca gccagattat ggattttgct gcaacgcgca aaggggtgat
    83341 gattttcgcc gccaccgttg aacacgcaaa agagattgtg ggattactac ccgccgaaga
    83401 tgctgcattg attactggcg acacccccgg cgctgagcgc gatgtgttaa ttgaagattt
    83461 taaagcccag cgttttcgct atctggtcaa cgtcgcagta ctgaccaccg gatttgacgc
    83521 cccgcacgtc gatcttatcg ccattctgcg ccctaccgaa tcagtgagtc tttaccaaca
    83581 gattgtcggg cgcggtctgc gtctcgctcc gggcaagact gattgtttaa tccttgatta
    83641 tgcgggtaat cctcacgatc tctacgcgcc ggaagttggt acaccgaaag gcaaaagtga
    83701 caacgttccg gtacaggttt tctgccctgc ctgcggtttt gccaacacct tttgggggaa
    83761 aacgaccgcc gacgggacat tgattgaaca ctttggtcgt cgctgtcagg gatggtttga
    83821 agatgacgac ggtcatcgcg aacagtgtga cttccgtttc cgttttaaaa attgcccgca
    83881 atgtaacgcg gaaaacgata ttgccgcccg ccgctgccgc gaatgtgaca ccgtactggt
    83941 tgatccggat gatatgttaa aagcggcgct acgactgaaa gacgcgctgg tattacgctg
    84001 tagcggcatg tctttacagc atgggcacga cgagaaaggc gaatggttga aaatcaccta
    84061 ttacgatgaa gacggcgcgg atgtgagtga gcgtttccgt ctgcaaacgc ccgcccagcg
    84121 taccgccttc gagcagcttt ttatccgccc gcatacgcgc acaccgggca tccctctgcg
    84181 ctggatcacc gccgccgata tcctcgccca gcaagcctta ctgcgacatc cggattttgt
    84241 cgtcgcccgc atgaaaggtc agtactggca agtgcgtgaa aaagtgttcg attacgaagg
    84301 tcgttttcgt cgggcgcacg aattacgcgg ttaatggtag ttttgattga tgtataaggc
    84361 gattgagtat agaatctcgc ccgcttttgc atacgcaaag cagatcactt acctgttgct
    84421 gggtcgcctg tagcaggatt aatttaagag agaaagaaat gtttactatc aacgcagaag
    84481 tacgtaaaga gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac aagttcccgg
    84541 caatcatcta cggtggcaaa gaagcaccgc tggctatcga gctggatcac gacaaagtca
    84601 tgaacatgca agctaaagct gaattctaca gcgaagttct gaccatcgtt gttgacggta
    84661 aagaaatcaa agttaaagct caggacgtac agcgtcaccc gtacaaaccg aagctgcagc
    84721 acatcgactt cgttcgcgct taattgctga ataagttgta aaaaaccccg ctccggcggg
    84781 gttttttgta tctgcagatt atgcctgatt acggtattgc tattttttgc aggccagata
    84841 aggcgttcac gccgcatctg gcattcagaa cacaaagccg attaattgcc gccagacgtc
    84901 cggcgctgca attggtcgcg caaattcggc ggcgtgcctt taatggtcaa ggtatcggtt
    84961 gctggatccc agaagatgcg ttcacctaac agcatcgcat caaagttaat agtcaagcca
    85021 ccgccgctac cggcaaactt cgtcaactga cgcagtgtgc tacggtctgc cgggaagctc
    85081 tcttccagtt cgtagccttt ttcagccgca aactccatga agctcacttc gctgacgcct
    85141 gccagctctt tcgacagcga ctccagttcg atctcttcgc ctgcctgcag ctgctcatta
    85201 cagtagctat aaacctgctg gcgcacgttc tgtcgctctg ctttatccag ttgtgcttcg
    85261 gcagtgaaat catcaaccgc ttgcaacagc ccacggtttt gcgctttcgc gtttaaacct
    85321 tcgctggccc cgaggaaatc cataaagaaa tccgccactt tgcgccctac tctccctttc
    85381 aggaaagtga gatagcgggt ggactctgga ttggtttccc attcggttaa atcgatacgc
    85441 gcaacaatat ccgcatgatt gatatcaaga taatgggttg ggttgatgtc gagattttcg
    85501 ttaacgcgca tactgctcag gttgctcaga actgccacca gcaaatactc caccgccaga
    85561 tagcgatagt ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt cgctaattcg
    85621 tcacgcaggc gaccggttgc cgcgcggcta aatgccagaa agtcctcttc accctgacgc
    85681 tgtaagcgca gcgtctgcgc cagttcactc tcttcgctaa acaagccgta agctttattt
    85741 ttggcgctat agacccgatg cagttctgcc accatctcaa cgacggtttc tgtcggttcc
    85801 agcaatgaat cgcgcaacac cagctcaagg ttctgctcat cacgcttgat aagctggtgc
    85861 agagcaatct ggttgatatc cagactcatg ataaactctc ctttaagacc gggcggtatt
    85921 caaccaccgc cggttaagcg acgcaataaa agatggcggt cattttacag aaggcggcgt
    85981 aacgagaatg atttaacgca taaaaaagca gaaaaaaaac cgttgctacg gtaatatgtt
    86041 gccctttcat caactaactg atttcgattt atgccacaaa tttcccgcta cagtgatgaa
    86101 caggttgaac aattgctcgc agagctgctc aacgtactgg aaaaacataa ggctccgacc
    86161 gacctttccc tgatggtgtt aggcaacatg gtcactaacc ttatcaacac cagcattgcc
    86221 ccggcccaac gccaggcaat tgccaactct tttgcccgcg ccttacagtc ctctatcaac
    86281 gaagacaaag cgcactaagg gaaacagata acaggttatg gtaactcatc gtcagcgcta
    86341 ccgtgaaaaa gtctcccaga tggtcagttg ggggcactgg tttgcactgt tcaatattct
    86401 gctttcgctc gtcattggca gccgttacct gtttatcgcc gactggccga ccacgcttgc
    86461 tggtcgcatt tattcctacg taagcattat cggccatttc agcttcctgg tgttcgccgc
    86521 ctacttgctg atcctcttcc cgctgacctt tatcgtcggc tcccagaggc tgatgaggtt
    86581 tttgtccgtc attctggcaa cggcgggaat gacgctatta ctgatcgata gcgaagtctt
    86641 tactcgtttc catctccatc ttaatcccat cgtctggcaa ctggttatca acccagacga
    86701 aaatgagatg gcgcgcgact ggcagctgat gttcatcagc gtgccggtta ttttattgct
    86761 tgaactggtg tttgcgacgt ggagctggaa aaagctgcgc agcctgacgc gtcgtcgtcg
    86821 cttcgcgcgt ccgctggccg cattcttatt tatcgccttt atcgcctcgc atgtggtgta
    86881 tatctgggcc gatgccaact tctatcgccc gatcaccatg cagcgcgcta acctgccgct
    86941 ttcgtacccg atgacggcgc gacgttttct tgagaagcat ggtctgcttg atgcgcagga
    87001 gtatcaacgc cgtcttattg agcaaggtaa tccagacgcc gtttccgttc agtatccgtt
    87061 aagcgaactg cgctatcgcg atatgggcac cgggcagaat gtgctgttga ttactgtcga
    87121 tggcctgaac tactcacgct tcgagaagca gatgcctgct ctggcaggtt ttgctgagca
    87181 aaatatttcg tttacgcgcc atatgagctc cggcaacact acagacaacg gcatctttgg
    87241 cctgttctat ggcatctcgc cgagctatat ggacggcatt ctgtcgaccc gtacgcctgc
    87301 ggcattaatt actgcgctta atcagcaagg ctatcagctg gggttattct catcagatgg
    87361 ctttaccagc ccgctgtatc gccaggcatt gttgtcagat ttctcgatgc cgagcgtacg
    87421 cacccaatcc gacgagcaga ccgccacgca gtggatcaac tggctgggcc gctacgcaca
    87481 agaagataac cgctggttct cgtgggtctc tttcaatggc actaacattg acgacagcaa
    87541 tcagcaggca tttgcacgga aatatagccg ggcggcaggc aatgtcgatg accagatcaa
    87601 ccgcgtgctc aatgcactgc gtgattctgg caaactggac aacacggtgg tgattatcac
    87661 tgccggtcgg ggtattccac tgagcgaaga ggaagaaacc tttgactggt cccacggtca
    87721 tctgcaggtg ccattagtga ttcactggcc aggcacgccg gcgcagcgta ttaatgcgct
    87781 gactgatcat accgatctga tgacgacgct gatgcaacgc ctgctacatg tcagcacacc
    87841 tgccagcgaa tattcgcaag gtcaggattt gttcaaccct caacgccgtc attactgggt
    87901 taccgcagcg gataacgata cgctggcaat taccaccccg aaaaagacgc tggtgctgaa
    87961 caataacggt aaataccgca cttacaactt acgtggtgaa agagtgaaag atgaaaaacc
    88021 acagttaagt ttgttattgc aagtactgac agacgagaag cgttttatcg ctaactgatt
    88081 aattataaat cagttagcga aatatcttac ttgcaatcgg tgtggaaaac ggtagtatta
    88141 acagccacga gtcggcacgt agcgcagcct ggtagcgcac cgtcatgggg tgtcgggggt
    88201 cggaggttca aatcctctcg tgccgaccaa aaatcccaaa aaaaaccaac ccttacggtt
    88261 ggttttttta tatctgcaat taattcgata aacagaccgt gacacatcac agcctgttta
    88321 ttttctgtta tcagaacgtc cagaccacac ccgcctgagt attccacggc gattctaccc
    88381 ctgcgccatt gccatagctg actgacaaat gaccgcttaa cgtcggggta aacgatgacc
    88441 ttatcccagc ctgataaacg ccacgggttc cagccacatc attattaaat ttgccatcgt
    88501 cattaacttt cacctggtta gaatcggcgt attcctgacg tacggcggct ttcagccagg
    88561 gttccagcgt cgtaccgttt tgcaggtcca tgtgatagct taccgccgtt cccgcttcag
    88621 cgcgtaatat ccgggtattt cccacatccg cgcgcatgcc gtttgataac gtgtagtcct
    88681 gaccatctgt ggtaaagccg gtaaaggcca gataaggtct aacactccac aatccgtcaa
    88741 cccaacggaa cccgctctcg acatgagcac ccgcgccgtt actattgtaa tcgccaaacg
    88801 ctgttgcccc attactcatc ttgccatgga tggtgttggc aaaacggtca actttcacca
    88861 ccccatcaac ataggcaccg ttctgatgct cccaaccggc ataagccccc agggtatagc
    88921 tatcgatatt accttttccg ccgcgatcaa aaccaatatc agaatgagag taaccaaaga
    88981 acaagccgcg aattgtactg ctttcttcac gggagaaacg gctatcgata ccgagcgtca
    89041 ggcccgtcaa tgtttgctca aaaccagctc ccgcatcagt ggtcacgttg ttgcgggtgt
    89101 taattgccga actccacatc gccgtatcgt aactaacgcc ttttacgcta ccaagacgct
    89161 cacgcacggt gtccagttct gcatcaaata ccagcggttg tgcggccgcc atattcagca
    89221 catcagtggt tgaaggggta atttgcgcgc gattctctgc caaactccag ctatggttgc
    89281 cattatccag caaggtatat tcatacgtac cgatatcaac aacgcctccg gcattgccca
    89341 acgtaaatgc agcatcaccg ccgcccgttg ttaccagtgt aaggctatct cctgctgccg
    89401 ggctggcacc ggtgtccgtc acgaatattt tgaaatcacc tgttgcctga ccggtgacgt
    89461 tgagctgatc gctctgatga ttagccatat cggtacgcat ataaaaattg ccgtttcccg
    89521 acagggtatt ggtggtcaac gtaatgaaat tttccgcact tgccgtcact gatgaacgag
    89581 tcactggctc atccatcata atcacatttg cgtcagacat cgcaaggttg gctaagttga
    89641 ggagggtgtt ttggttcgtc gtagtgatat cggcaatgat ggtccatacg ctattttcga
    89701 gcgaaacgtc cgctttgctg gtatccacgg caccatgcgt tctaaaagaa gcgccctcag
    89761 aaaccactac cttacccacc aacattgcgg gtgcataatt tatttgcggc ttcatgacct
    89821 caagaatgcc atcattccct ctgactgaaa cgttaaccat tgtgccagcc cagacgttag
    89881 cgcggccatt gttgataacc atattttcag agatggcttt agaggaatac gttatacttc
    89941 cattctgatc tgatcgaccg agatcatata ccgcatttgc ctgcactctc gtaccagtat
    90001 cctttcccag cgattgcata gtggcatgct catcgaggat agtgtcaatg gcctgcgtgt
    90061 cctccataac aataagcccg gaaccatcat ccagttcata attttttgac acaccatctt
    90121 ttatactaaa ttggccttta ctgtttgtac cactcacttc cagcgcattc gttgagacaa
    90181 ttaattttcc gcctgctttt ttatcaacgc cagttgcagt cccgccatcc ataacctcca
    90241 gtaagccgcc actatctaca gtagtattat aagcgaagtc attttcttca actcgcaaac
    90301 ttccgccgtt ttccaacagc atattgtttg caattccatt cttgatatcg aactgaccga
    90361 gacggtttgt tccgaatacc tccatgaccc gcgtggttgc tttaatagca ccgcctgctt
    90421 tctgatctac cgcaatcgct aatcccccct ctttaacctc cagtaatcct cccttttcga
    90481 tagcggcaag gacggtaatc ccatcttttt caacgattaa gcgcccgcct ttaatcgtgg
    90541 gatcattcgc tgcgccataa acctgtagca caccattatt gagggtagtc tggtttgccg
    90601 cgccacccat ttgaacaatt tgataaccat catttaatac ggagtattct gcggtgccat
    90661 tcatttcaat gtactgatac ccgccgttaa tttcagtatt actacttata ccaaattctt
    90721 taatatgctg ttctccgcca ggattgattt tcgtattatt ggcggtccca taaaccagct
    90781 ggatatcttt ttccaggaca acaccatcaa ctgtctcacc atactcaact gttgatgcca
    90841 atgctatggg tgaatataac gctgtagcca ccagaacaga tattgcggaa cgacaaagag
    90901 aaacagaacc agattgatgc attgagcttt catcctatga aattaattgc tgttaaaagc
    90961 attgggtaca gaaaataccc atagctccat acccggagtc agtttttaaa aactgtttaa
    91021 agaaatgcac aagtattgtg attgattttt tagttgtttt tcttgatgag aagctgatgc
    91081 aaaattccgt ctttataatg aaaatgatgc caaaacgaac gacaaggttg tagttttcac
    91141 tacatgtcca tacataaaat ggggtaacat tcacgcgcct ggtagcgtta ccaacgctac
    91201 gctcaaacat aatgattcta ataaaacctc aggagactac tatgcctgaa gcaacgcctt
    91261 ttcaggtgat gattgtggat gatcatccac ttatgcgacg cggtgttcgt cagttactgg
    91321 agcttgatcc tggctttgaa gtggtcgccg aagcgggcga cggcgcgagc gcgatcgatc
    91381 tggcgaatag actggatatc gacgtgatct tgctggatct gaatatgaaa ggtatgagtg
    91441 gcctggatac cctcaatgcc ttgcgcaggg atggcgttac cgcgcaaatt attatcctga
    91501 ccgtatcaga tgcctccagc gatgtctttg cgctgataga cgcaggcgca gacggttatc
    91561 tgttgaaaga cagcgacccg gaagtgttgc tggaagcgat tcgtgccgga gcgaaaggca
    91621 gcaaagtctt tagcgaacgc gtcaatcagt acttacatga acgtgaaatg tttggcgcgg
    91681 aagaagatcc cttcagcgtg ctgacggagc gcgagctgga tgttctgcac gagctggcac
    91741 aggggctgtc aaataaacag attgcctcgg tgttgaatat ttccgagcag acagtaaaag
    91801 tacatattcg caatctgctg cgcaaactca atgtccgctc acgcgtggca gccaccattc
    91861 tgttcctgca acaacgcggg gcacaataaa aatagcccga tggatttatc atcgggctga
    91921 gatttatgac aaacgcaaaa ctgcctgatg cgctacgctt atcaggccta cgcggatcga
    91981 tcaatttatt gaattcacac gattgtgtag gccggataag gcgttcacgc cgcatccggc
    92041 ataaattaag cgcactttgt catcagtctg gcctgatgga atcaccatcg ggcctctttt
    92101 tatttactct cctgcagcga caaatgttgc atcgcctgcg cgatactacg ttcaatcacc
    92161 gcacggcgag tatcgttggc aggtaagagt ttcaacatca tctcccacgc ggcaacggct
    92221 tcgccaaatc gctgctgctc aaaggcatta aacgcataca tgcttaacac acgaatattg
    92281 ctatggtccg ttctcaccaa ctggcgcagc agttcgccgc cgaggcggtt gtcgttggga
    92341 tcagatgaac gtgtcaacgc ttcagcgtat cccagtgcag catcgctgtt cttcggatcg
    92401 aggcgatacg cagtagcgta tgcatcggtg gcgatactgg cgttacccag cgccatgcca
    92461 acgcggccca acataatcca accttctata tctcccggat ttttttgcag ttgagtacgc
    92521 atccccagcg caaggcgcga catctcttct tcgttgagcg gatcggcttt cggatccagc
    92581 gccctgtcca gcaacgccgg agcctgtgcc gtggcctgct gccagatttt cacctgctga
    92641 taattgccag tctggtagta gctgacgcca gccacaatta acgccaccac aatacccggc
    92701 agataaacaa catatccggc acgcttacct tccggcacgc tttgttcagg aaacgcttcc
    92761 agcaccacgc gtacccgacg ccgcgagcgg gcgtaaatga cccaaccgcc aatgccaata
    92821 gccactactg gcagcaccca cagcagcacg gtcagcggcg ttaacggcgg atcgtaagtg
    92881 acgaagttgc cgtaacgcgc caccatataa tcgacaatct ctttcttact tttaccttcc
    92941 tgcatcagtt catacacttt ctggcgcagg tcggtggcaa tcatcgagtt ggaatcggca
    93001 atgctgttgt tctggcattt cgggcaacgc agttcttcag tgagctgacg gaactgttgt
    93061 tcctgtgctt catctttaaa ctgcaacacg tcgatggtcg ccagcgctga gccggagatc
    93121 atcagcatca gcacgcccaa taaaaacctc attgtgcggc ctccttactg tatttctccc
    93181 acagcggctt gatctcttct tcccagacgc gaggattgag atcgcccgca tggcgatagc
    93241 gaatgatgcc gttgccgtca ataagaaacg tttctggcgc gccatagaca ccgagatcca
    93301 gccctaacat gccatcgcca tcaaacaggc ttagcgcgta aggattgccc agctctttca
    93361 gccagctgat tgccttctgg cgatcgtctt tatagttcat gccgaccacg cggatgccct
    93421 gcgcagaaag ctgattcaga tattgatgtt ccgcacggca ggtcggacac caggtcgccc
    93481 agacgttaag cagcactggt ttgccctgag tcagcacatc cgcctgataa aactgccccg
    93541 gattgtccag tgattcgaga cgaaacttcg gcactggctt gccaatgagc gccgattcca
    93601 gattggtcgg atcatcccct tcggcattac gcgccagctg ccacagcagc gccgcggcaa
    93661 tcgccaggaa gataatcaac ggaattaaca atactttgcg cttcatacag cctccggcgc
    93721 agttttttgc ggtctcacgc gcttacgata gcgaggatca aacagacaca gcagtccgcc
    93781 caacgccatc atcagcccgc ccgcccaaat ccagcgaaca aatggtttgt agtaaagacg
    93841 cacggcccac gcgccgtttt ccagctcttc accgagggcc gcgtacaggt cacgcgtgat
    93901 gccgccgtca attgccgctt cggtcatcat cgacccggca gtgttgtaat aacgtttttc
    93961 cgcatacagc accgtttccg gtttaccctc tcgcgttacg ccgatagtcg ccacaccgcc
    94021 acgccagttc gggccagtca cctctttgac atcacggaag gtgaagcgat attcatgaat
    94081 atcgacgcta tcgccggact tcatgcgcac atcacgctca acgctatagt tctggctaaa
    94141 ggcaatgcca acaattgtca ctgccagccc caggtgagcc gccaccatcc cccaataact
    94201 gaaggtggtt ttcgtgccgc gtgaaatacg cagcacagct tccgcaattg ccagcaccgc
    94261 aatccagcag gccattgcca ggccgagcac cgtcatcgcc accactttgc tttcgaacag
    94321 ccacggcaac agcagagaca gcaccagcat agagatgaag gcgataatca ataaattgcg
    94381 gattttacgt gggcgatcgc gcccccagcg caccagagga ccgacaccaa gcagtagcgc
    94441 aaacggcacc atcagccagg taaacatggt gttgaagaac ggttcgccaa tcgaaatact
    94501 gcccagtccc agttgcttgt gcaccagcgg cagcagcgtc cccagcaaca ccaccagcat
    94561 cgcggcgacc agcaaaacgt tgttcgctaa cagcaaagat tcccgcgacc acagcgcatt
    94621 gtttacgcgt gagcgaactt tgtgtccacg cgcggcaaac agcagcagcg aaccgccaat
    94681 caccagcacc ataaaggcga ggataaacat accgcgcgcc ggatcagacg caaacgcgtg
    94741 taccgatacc agcacgccgg aacgcacgag gaaagtcccc aacagacaca acgagaaggc
    94801 actgattgcc agcagtaatg tccacgcttt gaagctggcg cgttgttcag tgaccgcaag
    94861 tgagtgcatc agcgcagtcc ccaccagcca cggcataaac gaggcgtttt ctaccggatc
    94921 ccagaaccac cagccgcccc agccgagttc gtaataggcc catgcggaac cgagcacaat
    94981 gccgagcgtc agaaagatcc acgctgccag tgtccacgga cgagtaaaac gcgcataagt
    95041 gctgtccaga cgcccgctca acaaagaagc aatggcaaaa gcaaacgcca ccgagaaacc
    95101 cacgtacccc atatagagca gaggcggatg gaagatcagc cccggatcct gcaacagcgg
    95161 gttaagatcg cgcccttcaa tcgggaagtt cggcagcgtg cgtgagaatg ggttagaggt
    95221 aaagagaatg aacagcaaaa agccgacact gaccatcccc attatcgcca gtacgcgggc
    95281 cacaatatcc agcggaatac gctgactaaa aatcgccacc gcaaaggtcc agccgctcat
    95341 cagcagcacc cacagcagta gcgagccttc atgcgcgccc caggtagccg ccacgcgata
    95401 ccacaccgga agctgggtat tggagttgct ggcaacatag gtgacggtaa agtcgttgac
    95461 cacgaaagca ttgaccagca ccagaaatgc gccagccaca gacataaaca gcagccaggc
    95521 aaacaagcgg gaagacgcca tcatgcgcgc atctccgcgc gccacgcccc atagcggata
    95581 cacggacagc agcagcgcaa ttcccagcgc caggcacagc agcccgttac caatttccgg
    95641 catcatgatg ctgggtcctt ataaacactc gccgggcgac ggtggttagc ttccatcgct
    95701 ttctcaactt ctggcggtgt gtagttttca tcgtgtttcg ccagcacttc tttcgcgagg
    95761 atatgattgc ctttttccag ctcgccctgc accacaacgc cctgcccttc acggaacaga
    95821 tccggcaaaa tgccttcgta agagacatcc actgagcctt cagcgtcgta aatggtgaag
    95881 gtcactttca gcgaattggg atcgcgctgc acgctacccg gcatcaccat cccgccaacg
    95941 cgcagacgct gcccaacttc cggcatctgc tgagtttcac gcttgccgta gagaatctcc
    96001 cccggcgtat aaaagagatc gatattcgag cgcagcgcat atagcaccag accgatagtc
    96061 agcgccaaac ctgccaacac agcacaggca atccacaagc ggtttttacg gcgaatattc
    96121 atgcagcctc ctgctgttgc gaagcacgta aacgcgcctc acgcgcccgc tgttgcgcca
    96181 cgccacgcag aattgcgcga tgctgcatca ccgagtgcac gactaaaacc accagcggaa
    96241 tcacggtcat caccaccgcc agccagacaa aaaaggcgta accgcccatt gcgaaaaatt
    96301 cattccagga agcaaatgca ggggtcattt acggcctctt ttcagtatca gttcactcac
    96361 ccacggacgg cgtttttcca tcagcaaaat taaattccgc atccgcatca gcgtcagcgt
    96421 ggcagacagc agcaggaagc caaaaatcga ccagcgcagc ggcgaacgca tcgcaggatc
    96481 gatactttgc tgcatccgcg ttgatccctg atgcagggtg ttccaccact ccacggagta
    96541 atgaataatc ggcagattca ccacgccaat cagcaccaga atacctgccg cacggcccgc
    96601 cagacggcgg tcgtcgaagg cgtgccacag ggcaatcaca cccacataca aaaacagcag
    96661 caccagttca gaagtcagac gtgcatccca tacccaccag gtgccccaca tcggtttccc
    96721 ccatgcagag ccggtaacca gggcaataaa ggtaaacacg gcaccaatgg gggccatcgc
    96781 cgccaccgcc aggttggcca ttttcatctg ccagacaagg ccaataaaag ccgccaccgc
    96841 cattgatgca taaatgccca tcgaccagat cgccgcaggc acatgcaggt agataatgcg
    96901 gtagctattt ccctgctgat aatcagccgg agcaaagccg aatccccaga tccagccgac
    96961 ggtaagcacg accacactgg caattgccag ccacggtata aaccagccac agatttgata
    97021 cagccgtggt gggatcgcca gttgatgcag tgttttccac atagtttcga taccagactc
    97081 gaacaaaaat cagtaatcca gcgttattga atgctgattc gtaacgctgc cgccgtcgca
    97141 aaaggactta atgtcgcggt gcctgccagc aacgcgccta aaattgccag atacccgtca
    97201 acgggcaaat gcatagaagc cgcgtccatc gcggcggtgg caaagatgag taatgggata
    97261 gtcagcggta acaccagtat gctgagcagc acaccaccgc gcttaagtcc cactgtcagc
    97321 gccacgcccg gtgcaccgag aaagccaagc gtaggcgttc ccagcagtag cgttaacgcc
    97381 atcacttgcc agccataaac atccattccc agtagcattg ctaccagtgg cgaaaggatg
    97441 agtaacggca gaccggttac catccagtgc gccattacct tcgccagcac aacggcgggc
    97501 aagggtaacg gcaacaacat caattgttca agactgccgt cctgcaaatc gtcacggaac
    97561 agtcgttcca gcgccagcaa ggatgaaagc agcgctgcaa cccagataat gcccggtgca
    97621 atacgcgcca gcagttgcgg ctccggaccg atactgagcg gaaaaagggt aattacaatc
    97681 aggaagaacc acagcgggtt ggcgatttcg gcgctatggc gaaacgctac gcgcagctca
    97741 agacggaaaa tgcgccagaa catcatgcgg cccccgtttg cgtcagtgaa atgcggcgaa
    97801 ttttactttc agcaacgttg agcggctggt gggtagtcag aatcacaatc cccccctgct
    97861 ccgtatgctg cgccatacgc tgggtcagac gatcgacgcc gttgacgtca atcgcggtaa
    97921 aaggctcgtc gaggatccat aacgtggcac gggtcagcca cagacgcgct aaagcgacgc
    97981 ggcgttgttg cccggcagaa agttgattta ccggaatatc ttcgaatccg gcaagcccgg
    98041 cctgcgccag ggcttccaga cattgtgcgg tatcgccatc gcgatgataa aagtgcagat
    98101 tttctaacgc cgtcagccgg gttttgatcc ccggctgatg gcctatccat aacaggtttt
    98161 gatggtagct gtcgcgtacc tgatgcaagg gctgcccttg ccagagaacc tcgcctgcgt
    98221 cagggcgaga caaccccgtc agcaaacgga gaagcgttgt cttccccgcg ccgttgctac
    98281 cggtgatttg tacccactct cctgcgttca gcgtaaatga caagccacta aataaggttc
    98341 gttcatcccg ctcacaaagt aactctctgg cttcaagcat acccacgcaa taaccctgtt
    98401 aaaaacctgg ctcgacttca cgcatatcgg ggagcttgtg cgctatccct ttatggcaat
    98461 caatacaggt ttgcccatct ttcaccgcct ggtcatgcat cttcgcggca accgatttct
    98521 gggcggttgt atccatatac tcgaagttgt gacagttacg gcactcctgc gagttattgt
    98581 ccttcatgcg ccgccactca ttctgtgcca tcgtcagacg atgagcttca aatttctgcg
    98641 gcgtgtcaat aacgccaaaa attttaccat acagctcttt acttgctttg agcttgcgta
    98701 tcatcttcgg cacaaactcg tgcggaacgt gacaatccgg acaggtcgca cgtacgccgc
    98761 tacggttgtt gtagtgcacg gaatccatgt attcctgata caccgtgtta cgcatttcgt
    98821 ggcagctaat gcagaactct tcggtattgg ctttttccat cccagtgtta aagccgcccc
    98881 agaagacgat gccgccgaca aaaccgatca acagcagcgt ccccagcgcc agacggctgg
    98941 gggtacgcca ccatttccac aggcgcttaa tcagaccagg cttacggtca gaatttccca
    99001 taataacctc ttatttcccg taaccttttg atggggtaaa ggtattcccc acgattggcg
    99061 cggtatcggc ctgcggtacg tgacattgca gacagaaata acgacgcgga gccacttctg
    99121 cgcccacttt gccgtcgctg tccataaaat gagtaggact gatacgcggc gcaccagtgg
    99181 tgcgataact ttcgacaccg tggcactgta agcagcggtt ggtattggtc gttacctgat
    99241 aaccttcaac gctatgcggg atcattggcg gctgattcac atagttcagc ggcatccgat
    99301 cctgctcttt tggcatccga atggcccctt cctgcgtccc ggatacttcc ggcgactggc
    99361 taaaatcgac gccattagcc gcccaaaccg cgccgcttac caccagggcc agcatcgccg
    99421 tccattgaca cagcgctttc ttcaggtcat ggcttttcat gatttcgctc ccgaactcca
    99481 tcgtgtagtt attgtaaata catcctcaga acagacatcc acgcagcgac cgcacgtcat
    99541 gcaatcgcgg ctggtgacct gcaccgggct ttgttcatcc agcaccgggg cacgtagcac
    99601 atgcggttcc gggcaaacat gaaaacaatc catacagcgg ttacatttct gccgatcgct
    99661 ggccgcaacg gtaatcacac ctttgctacc cagcacgcca tacagcgcgc caaccggaca
    99721 aatgtgcccg caccagccgt gttcaacgac cagtagatca aataaaaaca gcgcgagaat
    99781 aagcagcgcg ccgctgccga agcccattac caggctacgc cccatcaggg aaacggggtt
    99841 gatccattcc cagatgagcg tgccagttaa ggctgatccc accagaatga ccaccaacag
    99901 cacgtagcga atgtggcgag ggatcgtcgc agactgattg aggtcaaagc gcctgcgtaa
    99961 ccagttcgct aagtcggtaa tcgggttcag cgggcagacc cagctgcaaa ataatcgctt
   100021 tcccgccagg gcgtagagca cggtgataat caccgcaccg gtcaacgcca ccgtggcagg
   100081 cagatgacca ctggcaagac tttgcagcgt catcagcgga tcggtcagcg gaacggtgtc
   100141 gaataacagg ctactgctgt agttgccgtg caagatccac acaccaaacc acggaccgct
   100201 caaaaacatc cccagcacga agaactgaca aaggcgacgt aacaccagcc aacggtgact
   100261 gcgccaccag ccttttttct ccagcgcctc gcgcccggcg tcacgtttac gatttgccat
   100321 tgttcccctc cagccagccg aagcggtaat ggtgacctaa ctcccctttc gccagtgaca
   100381 gcggtaacac tttgattgcc ggttgttcca gtacgcacac cttttcgcac ttaccgcagc
   100441 cggtacaggc gtcgctgtga accgttggca gaaaacgggc gtgcttacca gtacgcgtgt
   100501 tgcgctccag ttccagggtg atggcctcat caattttcgg gcattcacga taacaaacat
   100561 cgcagcgcag cccctgaaag ttgagacagt tttcctggtc caccagtacc gccagcccca
   100621 tccgcgcgtc gtcgatcgat tcgatctcac gatccagcgc accgctcggg cacactttgg
   100681 cgcacggaat gtcctcacac atttcgcaag gaatatcccg tgcgacaaaa tatggcgtgc
   100741 ccgccgacag gccagaagcc agcgtcgcca gttttaaggt gtcgtaaggg caagcctgaa
   100801 cacactgacc gcaacgcaca caggcactgg caaaggcgtt ctcgtttatg gctccgggcg
   100861 ggcgcaaccg cacgccagat gcgcgtgcgg tttgctgttg taaccccagc gccacaccca
   100921 cggcagccag cccgcctgct gtgcgaacga catcgcgcag aaagcggcgg cgaccatttt
   100981 gaggtttcgc tgaccgggac ataatgactt acaccttctc cagtttgacc gcgcacttct
   101041 tgaagtccgt ctctttcgag agcggatcgg tcgcatccag cgtcagtttg ttaaccagct
   101101 gtgcggcgtc gaagaacggc atgtacacca ggccctgcgg cggacggtta cgaccgcgcg
   101161 tttcgacaat cgagatcact tcgccacggc gagaaaccac tttcactttg tcgccacgac
   101221 gcagatcgcg cgctttcgca tccagcgggt gaataaacag gaccgcttcc gggaaggcgc
   101281 ggtgcagttc cggcacacgg cgagtcatac tgccggtgtg ccagtgctcc aggacgcgtc
   101341 cggtagagag ccacaggtcg tactcttcat ccggtgcttc cgccgccggt tcgaacggca
   101401 gcgcgaagat caccgcttta ccatccggtt taccgtagaa cttatagcct tcgcccgctt
   101461 tcacgtacgg gtcgttacct tcgctgtaac gccactgcgt ttctttaccg ttcaccaccg
   101521 gccagcgcag accgcgcgct ttgtggtagt catcgaacgg cgcgagatcg tgaccgtgac
   101581 cgcgaccaaa ccatgcgtac tcttcgaaca gccctttttg cagatagaag cccagctcgc
   101641 gggattcatc gttcagctga tcttccgcca gttcggatac agggaatttg ctcacttcgg
   101701 gggtggcata cagaacttcg tacagcgttt tgccacgcag ttccggtttc ttcgccagca
   101761 gctcttccgg ccatacttct tcagttttga agcggcggga gaactggact aactgccaga
   101821 gatccgattt cgcttcgcct ggcgcctgta cctgctgacg ccagaactga gtacggcgtt
   101881 cggcgttacc gtaagcgccc tctttctcta cccacattgc ggtcggcagg atcaagtcgg
   101941 cggccagcgc actgactgtc ggatacggat cggagacgat gatgaagttg cgcggatcgc
   102001 gccagcccgg catacgctct tcattgatgt tcggcccggc ctgcatgttg ttggtacaca
   102061 tggtccagta aacattcagc ttgccgtctt tcagcgcacg gtcttgtgct accgcatgca
   102121 gaccgatttt cgccggaatg gtgccgctcg ggatattcca cttcttctcg cagatatcac
   102181 gatgtttctc gttagttacc accatgtccg caggcagacg gtgagcaaag gtgcccactt
   102241 cacgcgcagt accacacgcg gaaggctgcc cggtcaggga gaacggaccg caacccggct
   102301 gggaaatttt gccggtcagc aggtgcaggt tgtagaccag gttgttggcc cacacgccac
   102361 gagtatgctg gttgaagccc atcgtccagt aggagatgac tttcttgttc ggatcggcat
   102421 acagctgcgc cagttgttcc agctgatctt ttggtacgcc agtcatttcg gcggtttttt
   102481 ccagcgtata ctcggcaacg aaggctttgt aatcttcaaa gctcatcggt tcggaggcgt
   102541 cagagcccgg attcttcgct gctttttcca gcggatgggt cggacgtaaa ccgtagccga
   102601 tgtccgtcgc ccctttgcgc aggttaacgt gcttgctgaa gaagtcctga tttatcgcat
   102661 tgttttgaat gatatagttg gcgatgtagt tcaggatcac caggtcagat tgcggcgtaa
   102721 agatgatacc gttatccgcc agctcgaagc tacgatgctg gtaggttgaa agcaccgcca
   102781 cggtgacgtc ctggttagag agacgacggt tagtgatgcg tgaccagagg atcgggtgca
   102841 tctccgccat gtttgcgccc cacagcacaa acgcgtcagc ctgctcgatg tcgtcatagc
   102901 agcccatcgg ctcatccata ccaaaggtac gcataaagcc aactactgcc gacgccatac
   102961 agtgacgcgc gttcgggtcg atgttgttcg aacggaagcc cgctttaaac agcttggacg
   103021 cggcataacc ttcccagata gtccactgac cagaaccgaa cataccgatc gattccggcc
   103081 ctttttcttt cagggcggtt ttgaatttct cttccatcac atcgaaggcc tgatcccagg
   103141 tgattggggt aaattcgcct tctttgtcat atttaccgtt tttcatacgc agcagcggct
   103201 gcgtcaaacg gtctttaccg tacatgatct tgggcaggaa atagccctta atgcagttca
   103261 ggccacggtt aaccggtgcg tccgggtcgc cctgacaggc caccacacgc ccctgctgcg
   103321 ttccgaccag aacgccgcaa ccagtaccgc agaaacggca cggcgcttta tcccatttaa
   103381 tggcttcctg ctgaccaaca acggcgcggg caacgcccgg cacgctgaga ccggcagccg
   103441 ccgcagcggc cgcaacggcg ttagctttca taaagctacg acgactgagt ttcatggtgt
   103501 ttcctcacct tgctcttcct gctggtgata aaccagcgac accgccagca cgccctctac
   103561 gttgcgtact gactcaatgg tttggatcag cgtttcgctg tcttctgctt ccaccaccac
   103621 aatcaactga ccgctcggcg cgtcgctgac agcaacttca cagccgggaa aggcgttcag
   103681 ttgggtgctg atgtctgaaa ttcgttcgct tttggcctgc acgaccaggc tgcaaacttg
   103741 ccagttagtg tgcatggaga tactccgcag ttatggctga taccggacag ctggctgcac
   103801 atgctccaca tccgttgcaa agttggctat taagttgcgg ctggtagatc ccggagagcg
   103861 tcgggcgaaa gataattgcc attggctcgc agctatcctg acagcggcgg cattcaactg
   103921 actgatacgc caggcacgcg tccccaatgg tgaactgcaa atcccatgcc ctggtgtggc
   103981 gcggagaaaa tagcgattcg gggcaagcct gcgcgcaggc gtaacagaag ctgcactcgt
   104041 tatttttgaa attaacgctc ggatagccgc ccgcgccgcg ttgcagaatg ttgttttcac
   104101 aagcattaat acaggcgtcg cagcgggtgc aatgggtcag aaaatgagat tcatcaccgg
   104161 accagggcgg acggataccg ttactggctt tgcgccagcg accagtgagt atgccccgac
   104221 gggatgcatc aatcttcaca ttgaccttcc atcattaacg cgctctgaaa attgagagcg
   104281 accaaataaa ccgcataatt aataagccat ttttatagcc gctaagatat taaaggatgt
   104341 gtcaaagatg cataccccga tcgaggtaaa acctgtagca ggatcaaaag agtggcggga
   104401 agcgtggcaa aaacgggctt ttgctc
//
LOCUS       DAZFFG010000005        96249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFFG010000005 DAZFFG010000000
VERSION     DAZFFG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.005"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..505
                     /gene="yfgH"
                     /locus_tag="ACHJ7J_000501"
     CDS             <1..505
                     /gene="yfgH"
                     /locus_tag="ACHJ7J_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4998780.1"
                     /translation="KCLLPVTMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVN
                     IISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVG
                     AAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPN
                     TKCPEKS"
     gene            521..1060
                     /gene="yfgI"
                     /locus_tag="ACHJ7J_000502"
     CDS             521..1060
                     /gene="yfgI"
                     /locus_tag="ACHJ7J_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO4998781.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAAAAAADKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1153..2730)
                     /gene="guaA"
                     /locus_tag="ACHJ7J_000503"
     CDS             complement(1153..2730)
                     /gene="guaA"
                     /locus_tag="ACHJ7J_000503"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO4998782.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2799..4265)
                     /gene="guaB"
                     /locus_tag="ACHJ7J_000504"
     CDS             complement(2799..4265)
                     /gene="guaB"
                     /locus_tag="ACHJ7J_000504"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO4998783.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4427..5797
                     /gene="xseA"
                     /locus_tag="ACHJ7J_000505"
     CDS             4427..5797
                     /gene="xseA"
                     /locus_tag="ACHJ7J_000505"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO4998784.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5794..6009)
                     /gene="yfgJ"
                     /locus_tag="ACHJ7J_000506"
     CDS             complement(5794..6009)
                     /gene="yfgJ"
                     /locus_tag="ACHJ7J_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4998785.1"
                     /translation="MELHCPQCQHVLDQDNGHAHCPSCGEVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6078..7550)
                     /gene="der"
                     /locus_tag="ACHJ7J_000507"
     CDS             complement(6078..7550)
                     /gene="der"
                     /locus_tag="ACHJ7J_000507"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO4998786.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7668..8846)
                     /gene="bamB"
                     /locus_tag="ACHJ7J_000508"
     CDS             complement(7668..8846)
                     /gene="bamB"
                     /locus_tag="ACHJ7J_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO4998787.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8857..9477)
                     /gene="yfgM"
                     /locus_tag="ACHJ7J_000509"
     CDS             complement(8857..9477)
                     /gene="yfgM"
                     /locus_tag="ACHJ7J_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO4998788.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9495..10769)
                     /gene="hisS"
                     /locus_tag="ACHJ7J_000510"
     CDS             complement(9495..10769)
                     /gene="hisS"
                     /locus_tag="ACHJ7J_000510"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO4998789.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10880..11998)
                     /gene="ispG"
                     /locus_tag="ACHJ7J_000511"
                     /gene_synonym="gcpE"
     CDS             complement(10880..11998)
                     /gene="ispG"
                     /locus_tag="ACHJ7J_000511"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO4998790.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VSQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12025..13038)
                     /gene="rodZ"
                     /locus_tag="ACHJ7J_000512"
     CDS             complement(12025..13038)
                     /gene="rodZ"
                     /locus_tag="ACHJ7J_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO4998791.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13323..14477)
                     /gene="rlmN"
                     /locus_tag="ACHJ7J_000513"
     CDS             complement(13323..14477)
                     /gene="rlmN"
                     /locus_tag="ACHJ7J_000513"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO4998792.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14627..15058)
                     /gene="ndk"
                     /locus_tag="ACHJ7J_000514"
     CDS             complement(14627..15058)
                     /gene="ndk"
                     /locus_tag="ACHJ7J_000514"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO4998793.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15207..17519)
                     /gene="pbpC"
                     /locus_tag="ACHJ7J_000515"
     CDS             complement(15207..17519)
                     /gene="pbpC"
                     /locus_tag="ACHJ7J_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO4998794.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17520..22481)
                     /gene="yfhM"
                     /locus_tag="ACHJ7J_000516"
     CDS             complement(17520..22481)
                     /gene="yfhM"
                     /locus_tag="ACHJ7J_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO4998795.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWHWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQIGVALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERGLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSTEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRIVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22688..23533
                     /gene="sseA"
                     /locus_tag="ACHJ7J_000517"
     CDS             22688..23533
                     /gene="sseA"
                     /locus_tag="ACHJ7J_000517"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO4998796.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVALEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVNVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            23893..24018
                     /gene="timP"
                     /locus_tag="ACHJ7J_000518"
     CDS             23893..24018
                     /gene="timP"
                     /locus_tag="ACHJ7J_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO4998797.1"
                     /translation="MKIRCFCIVLIVSGALFTEVNNNRSLSSNNLLVVNNLQNSR"
     gene            24574..24699
                     /gene="timP"
                     /locus_tag="ACHJ7J_000519"
     CDS             24574..24699
                     /gene="timP"
                     /locus_tag="ACHJ7J_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO4998798.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGNNLLVINNLQSSK"
     gene            complement(24730..25506)
                     /gene="sseB"
                     /locus_tag="ACHJ7J_000520"
     CDS             complement(24730..25506)
                     /gene="sseB"
                     /locus_tag="ACHJ7J_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO4998799.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25649..26932)
                     /gene="pepB"
                     /locus_tag="ACHJ7J_000521"
     CDS             complement(25649..26932)
                     /gene="pepB"
                     /locus_tag="ACHJ7J_000521"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO4998800.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVFACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26991..27191)
                     /gene="iscX"
                     /locus_tag="ACHJ7J_000522"
     CDS             complement(26991..27191)
                     /gene="iscX"
                     /locus_tag="ACHJ7J_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO4998801.1"
                     /translation="MGLKWNDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(27203..27538)
                     /gene="fdx"
                     /locus_tag="ACHJ7J_000523"
     CDS             complement(27203..27538)
                     /gene="fdx"
                     /locus_tag="ACHJ7J_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO4998802.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27540..29390)
                     /gene="hscA"
                     /locus_tag="ACHJ7J_000524"
     CDS             complement(27540..29390)
                     /gene="hscA"
                     /locus_tag="ACHJ7J_000524"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO4998803.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALVADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29407..29922)
                     /gene="hscB"
                     /locus_tag="ACHJ7J_000525"
     CDS             complement(29407..29922)
                     /gene="hscB"
                     /locus_tag="ACHJ7J_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO4998804.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(30018..30341)
                     /gene="iscA"
                     /locus_tag="ACHJ7J_000526"
     CDS             complement(30018..30341)
                     /gene="iscA"
                     /locus_tag="ACHJ7J_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO4998805.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30358..30744)
                     /gene="iscU"
                     /locus_tag="ACHJ7J_000527"
     CDS             complement(30358..30744)
                     /gene="iscU"
                     /locus_tag="ACHJ7J_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO4998806.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30772..31986)
                     /gene="iscS"
                     /locus_tag="ACHJ7J_000528"
     CDS             complement(30772..31986)
                     /gene="iscS"
                     /locus_tag="ACHJ7J_000528"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO4998807.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(32098..32586)
                     /gene="iscR"
                     /locus_tag="ACHJ7J_000529"
     CDS             complement(32098..32586)
                     /gene="iscR"
                     /locus_tag="ACHJ7J_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO4998808.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32947..33687)
                     /gene="trmJ"
                     /locus_tag="ACHJ7J_000530"
     CDS             complement(32947..33687)
                     /gene="trmJ"
                     /locus_tag="ACHJ7J_000530"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO4998809.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33806..34609
                     /gene="suhB"
                     /locus_tag="ACHJ7J_000531"
     CDS             33806..34609
                     /gene="suhB"
                     /locus_tag="ACHJ7J_000531"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO4998810.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWIIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34754..35608
                     /gene="yfhR"
                     /locus_tag="ACHJ7J_000532"
     CDS             34754..35608
                     /gene="yfhR"
                     /locus_tag="ACHJ7J_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4998811.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35799..37079
                     /gene="csiE"
                     /locus_tag="ACHJ7J_000533"
     CDS             35799..37079
                     /gene="csiE"
                     /locus_tag="ACHJ7J_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO4998812.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(37071..38210)
                     /gene="hcaT"
                     /locus_tag="ACHJ7J_000534"
     CDS             complement(37071..38210)
                     /gene="hcaT"
                     /locus_tag="ACHJ7J_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO4998813.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRHNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38370..39260)
                     /gene="hcaR"
                     /locus_tag="ACHJ7J_000535"
     CDS             complement(38370..39260)
                     /gene="hcaR"
                     /locus_tag="ACHJ7J_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO4998814.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            39396..40757
                     /gene="hcaE"
                     /locus_tag="ACHJ7J_000536"
     CDS             39396..40757
                     /gene="hcaE"
                     /locus_tag="ACHJ7J_000536"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO4998815.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40754..41272
                     /gene="hcaF"
                     /locus_tag="ACHJ7J_000537"
     CDS             40754..41272
                     /gene="hcaF"
                     /locus_tag="ACHJ7J_000537"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO4998816.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            41272..41592
                     /gene="hcaC"
                     /locus_tag="ACHJ7J_000538"
     CDS             41272..41592
                     /gene="hcaC"
                     /locus_tag="ACHJ7J_000538"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO4998817.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41589..42401
                     /gene="hcaB"
                     /locus_tag="ACHJ7J_000539"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41589..42401
                     /gene="hcaB"
                     /locus_tag="ACHJ7J_000539"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO4998818.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42411..43613
                     /gene="hcaD"
                     /locus_tag="ACHJ7J_000540"
     CDS             42411..43613
                     /gene="hcaD"
                     /locus_tag="ACHJ7J_000540"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO4998819.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGEVHLFSDEQHLPYER
                     PPLSKFMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASTTQHRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            43710..44132
                     /gene="yphA"
                     /locus_tag="ACHJ7J_000541"
     CDS             43710..44132
                     /gene="yphA"
                     /locus_tag="ACHJ7J_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4998820.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(44180..45052)
                     /gene="yphB"
                     /locus_tag="ACHJ7J_000542"
     CDS             complement(44180..45052)
                     /gene="yphB"
                     /locus_tag="ACHJ7J_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO4998821.1"
                     /translation="MTIYTLSHGGLTLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNHFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCASCSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(45064..46158)
                     /gene="yphC"
                     /locus_tag="ACHJ7J_000543"
     CDS             complement(45064..46158)
                     /gene="yphC"
                     /locus_tag="ACHJ7J_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4998822.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(46191..47189)
                     /gene="yphD"
                     /locus_tag="ACHJ7J_000544"
     CDS             complement(46191..47189)
                     /gene="yphD"
                     /locus_tag="ACHJ7J_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4998823.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLGFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(47213..48724)
                     /gene="yphE"
                     /locus_tag="ACHJ7J_000545"
     CDS             complement(47213..48724)
                     /gene="yphE"
                     /locus_tag="ACHJ7J_000545"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4998824.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48747..49730)
                     /gene="yphF"
                     /locus_tag="ACHJ7J_000546"
     CDS             complement(48747..49730)
                     /gene="yphF"
                     /locus_tag="ACHJ7J_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO4998825.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49827..53108)
                     /gene="yphG"
                     /locus_tag="ACHJ7J_000547"
     CDS             complement(49827..53108)
                     /gene="yphG"
                     /locus_tag="ACHJ7J_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO4998826.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHGLNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            53226..54419
                     /gene="yphH"
                     /locus_tag="ACHJ7J_000548"
     CDS             53226..54419
                     /gene="yphH"
                     /locus_tag="ACHJ7J_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO4998827.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54617..55870)
                     /gene="glyA"
                     /locus_tag="ACHJ7J_000549"
     CDS             complement(54617..55870)
                     /gene="glyA"
                     /locus_tag="ACHJ7J_000549"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO4998828.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            56198..57388
                     /gene="hmpA"
                     /locus_tag="ACHJ7J_000550"
     CDS             56198..57388
                     /gene="hmpA"
                     /locus_tag="ACHJ7J_000550"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO4998829.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVNGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(57433..57771)
                     /gene="glnB"
                     /locus_tag="ACHJ7J_000551"
     CDS             complement(57433..57771)
                     /gene="glnB"
                     /locus_tag="ACHJ7J_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO4998830.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57832..59166)
                     /gene="glrR"
                     /locus_tag="ACHJ7J_000552"
     CDS             complement(57832..59166)
                     /gene="glrR"
                     /locus_tag="ACHJ7J_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO4998831.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(59156..59869)
                     /gene="qseG"
                     /locus_tag="ACHJ7J_000553"
     CDS             complement(59156..59869)
                     /gene="qseG"
                     /locus_tag="ACHJ7J_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO4998832.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(60034..61461)
                     /gene="qseE"
                     /locus_tag="ACHJ7J_000554"
     CDS             complement(60034..61461)
                     /gene="qseE"
                     /locus_tag="ACHJ7J_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO4998833.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADGAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(62020..65907)
                     /gene="purL"
                     /locus_tag="ACHJ7J_000555"
                     /gene_synonym="purI"
     CDS             complement(62020..65907)
                     /gene="purL"
                     /locus_tag="ACHJ7J_000555"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO4998834.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            66165..67721
                     /gene="mltF"
                     /locus_tag="ACHJ7J_000556"
     CDS             66165..67721
                     /gene="mltF"
                     /locus_tag="ACHJ7J_000556"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO4998835.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67718..68221)
                     /gene="tadA"
                     /locus_tag="ACHJ7J_000557"
     CDS             complement(67718..68221)
                     /gene="tadA"
                     /locus_tag="ACHJ7J_000557"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO4998836.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(68279..68914)
                     /gene="yfhb"
                     /locus_tag="ACHJ7J_000558"
     CDS             complement(68279..68914)
                     /gene="yfhb"
                     /locus_tag="ACHJ7J_000558"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO4998837.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPVIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            69123..69971
                     /gene="yfhH"
                     /locus_tag="ACHJ7J_000559"
     CDS             69123..69971
                     /gene="yfhH"
                     /locus_tag="ACHJ7J_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4998838.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            70027..70287
                     /gene="yfhL"
                     /locus_tag="ACHJ7J_000560"
     CDS             70027..70287
                     /gene="yfhL"
                     /locus_tag="ACHJ7J_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO4998839.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(70481..70561)
                     /gene="shoB"
                     /locus_tag="ACHJ7J_000561"
     CDS             complement(70481..70561)
                     /gene="shoB"
                     /locus_tag="ACHJ7J_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO4998840.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70982..71362)
                     /gene="acpS"
                     /locus_tag="ACHJ7J_000562"
     CDS             complement(70982..71362)
                     /gene="acpS"
                     /locus_tag="ACHJ7J_000562"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO4998841.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(71362..72093)
                     /gene="pdxJ"
                     /locus_tag="ACHJ7J_000563"
     CDS             complement(71362..72093)
                     /gene="pdxJ"
                     /locus_tag="ACHJ7J_000563"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO4998842.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(72105..72833)
                     /gene="recO"
                     /locus_tag="ACHJ7J_000564"
     CDS             complement(72105..72833)
                     /gene="recO"
                     /locus_tag="ACHJ7J_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO4998843.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72845..73750)
                     /gene="era"
                     /locus_tag="ACHJ7J_000565"
     CDS             complement(72845..73750)
                     /gene="era"
                     /locus_tag="ACHJ7J_000565"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO4998844.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73747..74427)
                     /gene="rnc"
                     /locus_tag="ACHJ7J_000566"
     CDS             complement(73747..74427)
                     /gene="rnc"
                     /locus_tag="ACHJ7J_000566"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO4998845.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(74700..75674)
                     /gene="lepB"
                     /locus_tag="ACHJ7J_000567"
     CDS             complement(74700..75674)
                     /gene="lepB"
                     /locus_tag="ACHJ7J_000567"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO4998846.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(75690..77489)
                     /gene="lepA"
                     /locus_tag="ACHJ7J_000568"
     CDS             complement(75690..77489)
                     /gene="lepA"
                     /locus_tag="ACHJ7J_000568"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO4998847.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(77687..78166)
                     /gene="rseC"
                     /locus_tag="ACHJ7J_000569"
     CDS             complement(77687..78166)
                     /gene="rseC"
                     /locus_tag="ACHJ7J_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO4998848.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLLLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(78163..79119)
                     /gene="rseB"
                     /locus_tag="ACHJ7J_000570"
     CDS             complement(78163..79119)
                     /gene="rseB"
                     /locus_tag="ACHJ7J_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO4998849.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(79119..79769)
                     /gene="rseA"
                     /locus_tag="ACHJ7J_000571"
     CDS             complement(79119..79769)
                     /gene="rseA"
                     /locus_tag="ACHJ7J_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO4998850.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79802..80377)
                     /gene="rpoE"
                     /locus_tag="ACHJ7J_000572"
     CDS             complement(79802..80377)
                     /gene="rpoE"
                     /locus_tag="ACHJ7J_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO4998851.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(80374..80529)
                     /gene="rseD"
                     /locus_tag="ACHJ7J_000573"
     CDS             complement(80374..80529)
                     /gene="rseD"
                     /locus_tag="ACHJ7J_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO4998852.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80785..82407
                     /gene="nadB"
                     /locus_tag="ACHJ7J_000574"
     CDS             80785..82407
                     /gene="nadB"
                     /locus_tag="ACHJ7J_000574"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO4998853.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCSGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(82392..83129)
                     /gene="trmN"
                     /locus_tag="ACHJ7J_000575"
     CDS             complement(82392..83129)
                     /gene="trmN"
                     /locus_tag="ACHJ7J_000575"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO4998854.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGTGSGLLALMLAQRTDDSVMIDAVELESEAATQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            83261..84595
                     /gene="srmB"
                     /locus_tag="ACHJ7J_000576"
     CDS             83261..84595
                     /gene="srmB"
                     /locus_tag="ACHJ7J_000576"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO4998855.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(84628..85509)
                     /gene="yfiE"
                     /locus_tag="ACHJ7J_000577"
     CDS             complement(84628..85509)
                     /gene="yfiE"
                     /locus_tag="ACHJ7J_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4998856.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPYIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRSTVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFLPG"
     gene            85612..86199
                     /gene="eamB"
                     /locus_tag="ACHJ7J_000578"
     CDS             85612..86199
                     /gene="eamB"
                     /locus_tag="ACHJ7J_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO4998857.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLTVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(86255..86638)
                     /gene="grcA"
                     /locus_tag="ACHJ7J_000579"
     CDS             complement(86255..86638)
                     /gene="grcA"
                     /locus_tag="ACHJ7J_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO4998858.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86943..87632
                     /gene="ung"
                     /locus_tag="ACHJ7J_000580"
     CDS             86943..87632
                     /gene="ung"
                     /locus_tag="ACHJ7J_000580"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO4998859.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(87680..88717)
                     /gene="yfiF"
                     /locus_tag="ACHJ7J_000581"
     CDS             complement(87680..88717)
                     /gene="yfiF"
                     /locus_tag="ACHJ7J_000581"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4998860.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88924..89343
                     /gene="trxC"
                     /locus_tag="ACHJ7J_000582"
     CDS             88924..89343
                     /gene="trxC"
                     /locus_tag="ACHJ7J_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO4998861.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            89412..90110
                     /gene="tapT"
                     /locus_tag="ACHJ7J_000583"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             89412..90110
                     /gene="tapT"
                     /locus_tag="ACHJ7J_000583"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO4998862.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAEAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            90142..92802
                     /gene="pat"
                     /locus_tag="ACHJ7J_000584"
     CDS             90142..92802
                     /gene="pat"
                     /locus_tag="ACHJ7J_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO4998863.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92916..94271
                     /gene="pssA"
                     /locus_tag="ACHJ7J_000585"
     CDS             92916..94271
                     /gene="pssA"
                     /locus_tag="ACHJ7J_000585"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO4998864.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            94317..94640
                     /gene="yfiM"
                     /locus_tag="ACHJ7J_000586"
     CDS             94317..94640
                     /gene="yfiM"
                     /locus_tag="ACHJ7J_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO4998865.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(94637..95935)
                     /gene="kgtP"
                     /locus_tag="ACHJ7J_000587"
     CDS             complement(94637..95935)
                     /gene="kgtP"
                     /locus_tag="ACHJ7J_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO4998866.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(96052..>96249)
                     /locus_tag="ACHJ7J_000588"
     CDS             complement(96052..>96249)
                     /locus_tag="ACHJ7J_000588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998867.1"
                     /translation="KRKAILTDGLFALVKKMPDATLRVLYSHICQIQQRIRLPQLAHL
                     HTCSPHQKFILKLLNNHFPAN"
BASE COUNT        22990 a        26254 c        24129 g        22876 t
ORIGIN      
        1 aaaatgtctt ctgcctgtga caatgttagc gtcattcact ctggcaggat gccagtcaaa
       61 tgctgacgat catgccgccg atgtttatca aaccgatcaa ctgaatacca aacaagaaac
      121 taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa
      181 taaacggaac gcacaagcct tcggcgcgct tattggcgca gtcgctggcg gtgttatcgg
      241 ccacaacgtc gggtctggca gcaattccgg aacgacggca ggtgcagttg gcggcggagc
      301 tgtaggcgcg gcagcgggtt ctatggtgaa tgataaaacc ttagtggaag gtgtttcttt
      361 aacgtataag gaaggcacca aagtgtatac ctctactcag gtgggtaaag agtgccagtt
      421 tacgacaggt ttagccgttg ttattaccac gacgtataac gaaacgcgta ttcagccaaa
      481 taccaaatgt cctgaaaaga gctaataatc aggaggagtc atgaagaaag tttttctttg
      541 cgccatctta gcctccttaa gctatccggc tatcgcctca tcattgcagg atcaactctc
      601 tgctgtcgca gaagcggaac agcaaggtaa aaatgaagag caaaggcagc atgacgaatg
      661 ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa atgcccaggc
      721 cgccgctaac aaaagagcgg cggcagcagc agcggataag aaagctcgtc aggataaact
      781 ggacgccgaa gccaccgcgg ataaaaaacg cgatcaaagt tatgaagatg agctgcgcag
      841 cttagagatt cagaaacaaa agctggcgct ggcgaaagaa gaagcccgcg ttaagcgcga
      901 aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg tggtgcaatc
      961 tgaagctgac gccaacagaa atatgactga aggcggtcgc gatctgatga aaagcgtggg
     1021 caaagcagaa gagaacaaat cggacagctg gtttaattaa tcgatgttac taacttcaag
     1081 cctatgattc ttgaagataa aaaaccctct gtagtaacag agggttttgt tcattcatag
     1141 tgcagggtca aatcattccc actcaatggt agctggcggc ttgccgctga tgtcatacac
     1201 cacgcgggaa ataccgttca cttcattgat aatgcggttg gaaacgcgac cgaggaaatc
     1261 gtacggcaga tgcgcccagt gtgcggtcat aaagtcgatg gtttcgacag cacgcagaga
     1321 gacaacccag tcatacttac gaccatcgcc cattacgcca acggaacgta ctggcaggaa
     1381 gacggtgaac gcctggctga ctttgtcgta cagatccgct ttacgcagtt cttcaatgaa
     1441 gatggcgtca gcacggcgca gcaggtcaca gtactctttc ttcacttcac ccagaacacg
     1501 aacgccaagg cctggtcccg ggaacgggtg acggtacagc atgtcgtacg gtagacccag
     1561 ctccagacca atcttacgca cttcgtcttt gaacagctct ttcagcggtt caaccaggcc
     1621 catcttcatc tctttcggca ggccgcccac gttgtggtga gatttgatga cgtgtgcttt
     1681 accggttgca gacgccgcag attcgataac gtcagggtag atggtgccct gtgccagcca
     1741 cttcacgtct tccagtttca gcgcttcttc atcgaatact tcaacgaaaa cgcgaccgat
     1801 gattttacgt tttgcttccg gatcgttttc gccagccagc gctgacagga agcgatcttc
     1861 tgccggtacg tgaacaatgt taagaccaaa gtgatcgcca aacatatcca gaacctgctc
     1921 tgcttcgttg agacgcagca ggccgttgtc gacgaatacg caagtcaggt ttttaccgat
     1981 agcgcggtgc agcagcattg cggttacgga ggaatccaca ccaccagaga ggccgaggat
     2041 gactttatcg tcgcctacct gctcgcggat gcgagctaca gcatcgtcga taattttcgc
     2101 tggcgtccac agggcttcac actggcagat atcacgcaca aaacgctcca gcatgcgcat
     2161 accctggcgg gtgtgagtca cttccgggtg gaactgtacg ccatagaagc gtttttcttc
     2221 gttagccata atggcaaacg ggcagctttc ggtgctggct acggtgacga agtcggacgg
     2281 gatagcggta actttatcgc cgtggctcat ccagacatcg agcagcggtt taccgtctgc
     2341 ggtcagcgca tcttcgatac cgcgaaccag tgcgctgtcg tttacgactt caacctgcgc
     2401 gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg cccaactgca ttgccatggt
     2461 ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct tcaaagacat actgcggcgc
     2521 acgcggactg ttttcttcag tagtactttc cgggccgccg gaaagaataa tgccgcttgg
     2581 attgaagtca cgaatttgtg cttctgtcac atcccacgcc cacagttcgc agtaaacacc
     2641 cagctcacgc acgcggcgcg caaccagttg agtgtactga gaaccgaagt ccagaatgag
     2701 gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa gtgaaacaga
     2761 taatataaat cgcccgacat gaagtcgggc gaagagaatc aggagcccag acggtagttc
     2821 ggggactctt tagtaatggt cacgtcgtga acgtggcttt cctgaatgcc cgcaccgctg
     2881 atacgtacaa actccgcttt agtacgcagt tcgtcgatag taccacagcc ggtcaaaccc
     2941 atacaggagc gcaggccgcc catctgctgg tgaatgatct ctttcaggcg acctttataa
     3001 gctacgcgac cttcgatacc ttccggcacc agtttgtcgg cagcgttatc gctctggaaa
     3061 taacggtcag aggaaccttt ggacatcgcg cccagggaac ccataccacg gtaagatttg
     3121 taagaacggc cctggtagag ttcgatttca cccggagatt cttcagtacc cgccagcatg
     3181 gaacctacca tcaccgcgct tgcgccagcg gcgatagctt tggcgatgtc gccggagaag
     3241 cgaataccac catctgcgat aaccggaata ccggtgcctt ccagggcttc aactgcgtca
     3301 gcaacggcag tgatctgcgg aacacctacg ccagtaacga tacgagtcgt acagatagaa
     3361 ccagggccga taccgacttt aaccgcgctg caaccagctt ctgccagagc gcgtgcacct
     3421 gcagctgttg ccacgttgcc gccgatgatt tgcagatccg gatatttagc acgggtttca
     3481 cggatacgct gcaaaacgcc ttctgagtga ccgtgggagg agtcgatcag cagaacgtca
     3541 acgccagcgg caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc gccaactgca
     3601 gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg ttccgctttc
     3661 tggaaatctt tcacggtgat catgccgatc aggtggaatt cgtcatcaac caccagcgct
     3721 ttttcaacgc gtttttcgtg catttttgcc agcaccactt cacgggcttc accttcacgc
     3781 acggtgacca gacgctcttt cggcgtcatg taaacgctaa ccggctggtt caggtcggta
     3841 acaaaacgca cgtcacgacc agtgataata cccaccagtt cgttttcttc ggtaacgacc
     3901 ggatagcccg caaaaccgtt acgctcggtc agttctttca cttcgcgcag cgtcgtggtt
     3961 ggcagaacag tctgcggatc agtcaccacg ccagattcgt gttttttcac acggcgaact
     4021 tcttctgcct ggcgttcaat ggacatgttt ttgtggataa agccgatacc gccttcctga
     4081 gccagagcaa tagccaggcg cgcttccgtt acggtatcca ttgctgcgga aagcataggg
     4141 atattcagac gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt cggcagaacg
     4201 gtagaatgag caggaacgag gagaacgtcg tcaaacgtca gagcttcttt agcgatacgt
     4261 agcatgggca atatctcgac cagagtggtt aataaatatt gccgcggcat tatacagagc
     4321 gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg ctatccccga agggcgggtt
     4381 actatctact gaataacctg ctgatttaga atttgatctc gctcacatgt taccttctca
     4441 atcccctgca atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc tgcttgagca
     4501 tgagatggga caggtttgga tcagcggcga aatctctaat ttcacacaac cggcttccgg
     4561 tcactggtac tttacactca aagacgacac cgcccaggta cgctgcgcga tgttccgcaa
     4621 cagcaaccgc cgggtgacct tccgcccaca gcatgggcaa caagttttag ttcgcgccaa
     4681 tattacgctc tacgagccgc gcggcgacta ccagataatc gttgagagta tgcagccggc
     4741 cggtgaaggg ctgctgcaac agaagtacga acagctcaaa gcgaagttgc aggctgaagg
     4801 tttgttcgat cagcaataca aaaaaccact tccctcccct gcgcattgcg ttggtgtgat
     4861 cacctcaaaa accggtgctg cgctacatga tattttgcat gtgttaaaac gtcgcgatcc
     4921 ttctctgccg gtgataatct accccaccgc cgttcagggc gatgacgcgc cggggcaaat
     4981 tgttcgcgcc attgaactgg cgaatcagcg caatgagtgc gacgtgttga tcgttgggcg
     5041 cggcggcggt tcgctggaag atttatggag ttttaacgac gaacgcgtag cgcgggcgat
     5101 ttttgccagc cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg tgaccattgc
     5161 cgattttgtt gccgatctgc gtgcaccaac accgtcggct gccgccgaag tggtgagccg
     5221 taatcagcaa gagttactgc gccaggtgca atcgacccgt caacggctgg agatggcgat
     5281 ggattattat ctcgccaacc gcacacgtcg ctttacgcaa attcatcacc gattacagca
     5341 acagcatccg cagctccggc tggcacgcca gcaaaccatg cttgaacgcc tgcaaaaacg
     5401 gatgagcttt gcgctggaaa atcaacttaa gcgtgccggg caacagcagc agcgattaac
     5461 acagcggctg aatcagcaaa atccacagcc gaagattcat cgcgcgcaaa cgcgcattca
     5521 gcaactggaa tatcgtttag cagaaaccct gcgcgcacag cttagcgcca cgcgtgaacg
     5581 tttcggtaat gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc tggcgcgtgg
     5641 atacagcgtt actactgcta ctgacggcaa tgtactgaaa aaagtgaagc aagttaaagc
     5701 gggtgaaatg ctaaccacac gtctggaaga cggctggata gaaagtgaag taaaaaacat
     5761 ccagccggta aaaaaatcgc gtaaaaaggt tcattaagcc agcacgaatt caacgcgttt
     5821 tttcgatatc aagccatgcc cgtgctgaca gaagtaatct accgcaccgc aggcttttaa
     5881 cacctgcaac ggttgatggc agtccgggca aagagctttc atttcaataa cttccccgca
     5941 ggatgggcaa tgcgcatggc cgttatcctg atcaagcaca tgctgacatt gtggacaatg
     6001 cagttccata tgaattcctc cggtagccac ccctctacat tcatagaggg aatggcagat
     6061 aaaatactta cggataatta tttatttttc ttgatgtgct tcatcagacg cttacgttta
     6121 cgcatctggg ttggcgtcag ggtgttacgc ttgttcgcat acgggttttc cccttctttg
     6181 aactgaatac gaatcggcga tcccattacg tccagcgatt tgcggaagta gttcatcaag
     6241 taacgcttgt aggaatcagg caggtctttc acctgattac cgtgaatcac cacaatcggc
     6301 ggattataac caccggcgtg ggcatatttc agcttcacac gacgaccgcg taccagcggc
     6361 ggttggtgat cttcaacagc catcgtcatg atgcgcgtca gcatagaggt ccccacacga
     6421 cgggtggagc tgtcatacgc ttcacgtact gattcaaaca agttaccaac accactgccg
     6481 tgcaaggcag agataaagtg cacacgagca aaatcgataa agcccagacg gaagtccagc
     6541 gtttctttca cctgctcttt cacttcctgg ctcaggccat cccacttatt caccacaatg
     6601 acaagtgagc gcccactatt gagaataaag cccagcagcg agagatcctg atcggaaata
     6661 ccttcgcgcg catcaatcac taacatcacc acgttggcgt cttcaatggc ctgcaacgtt
     6721 ttgattacgg agaatttctc tacagcatcg gtgattttgc cgcgtttacg tacgccagcg
     6781 gtgtcaatga gcacatactc acgtccatcg cgttccattg gaatgtagat actgtcacgc
     6841 gtcgtaccag gcatgtcata aacaacaacg cgctcttcgc caagaatacg gttagtgagt
     6901 gtagacttac ctacgttcgg acgacccaca atcgccagtt tgatcggcag actttgcggg
     6961 tcgaagtcgt cttcctcttc ttcttcgccg ttctcttccg cttcaaattg cgcccagtat
     7021 tcagcgtctt cgtcgacttc ctcttgcggt gcgagatctt ccatccacgg cagtagcaca
     7081 tgctccagca gacttaatac gccacgaccg tgagacgcgg cgatcgggta gatttcacct
     7141 aaaccaagcg agtagaaatc aaccactgcc tgatcgggat ccagaccgtc agttttgttt
     7201 gccaccagga aggtcggttt ttcacgggag cgcagatgtt tggcaatcgc ttcatctgcc
     7261 ggcatcaggc ccgcgcgcgc atccaccata aacagtacga cgtccgcttc ttcaatcgcc
     7321 agcagcgact gttccgccat gcgggtttct acaccgtctt ctgtgccatc aatcccgccg
     7381 gtatcaatac agataaactc acggccttca atttccgcac gaccgtactt acggtcacga
     7441 gtcagacccg ggaaatccgc aaccagcgca tctcgggtgc gagttagacg gttaaataac
     7501 gtggattttc ctacgttagg gcgcccgaca agcgcgacca caggtaccat gtttaaagcc
     7561 tcatttttat aaatcatcag acaacgcacg ctatattcgc gtcgttgtta aaaacaggaa
     7621 aacggcccct gtccaggagc cgttttcaaa gtgaacgaca gagacgatta acgtgtaata
     7681 gagtacacgg ttccgtcttt tgcctggatc agcagtttgc cgtcagcggc aaccggttca
     7741 gtctggaaac cggaactatc aactttttgc tgggcaacga aacgaccatc ttcgacgtta
     7801 atccagtgca aataaccttc actgtcaccg accaccaggt tgccattata cagcaccgga
     7861 gaagtcagca ggcgatgcag cagatcgctt tgtgtccaca gcgtaacgcc gccatcaatg
     7921 gtcaacgcca tcacccggtc attttgatcg accagataga tgcgattgcc gtcgacgatg
     7981 aaatcattca ccgaacccag ttcgcgtttc cacataatct gaccactgcg cagatcaagc
     8041 gccgtcaggt taccattata ggccagcgcg aaaacaacgc cgttaacaac gacgggagtc
     8101 gtgtcaacat cgctcagacg gtcaatttcg gtagaaccgg tcgcctggga aatacgctgc
     8161 tgccaaatca tctggccctg ttccatcagc actgcgctga cgcgaccatt atcgcccccc
     8221 acgacggccg caccaaaagc cgttgccggc gcagactcgc cacgcaaaga gagcgaaggc
     8281 atatcgaggt taactgtcca tttgacagcg ccgtcagctt cgttcagcgc ttgtaactga
     8341 ccgttactgg tgtggattaa caccagaccg tcgctgacca ccgggcgcga aagtgcttca
     8401 cccgcgactt tagtttgcca tgccacagta ccatcgctgg tattcagcgc gtaaacctgc
     8461 gccttttcgc tgccaatgta gacatgccca ccagacacgg tcacaccgcc agaaagtaat
     8521 gcaggctctt tagagaacca gccatctttc tcggccaggc tgacagacca gatttctttg
     8581 ccatcatccg cattcagcgc ttttactaaa ccagcgcggt ccgctgcata gacaacgttg
     8641 tccgccagtg ccggatgaag attggaatag aagttgccaa tgccgctacc aacggaagtg
     8701 ctccacgccg tggtcggcgt aaactggttt tcaacggttg gcaatgggga catctttacc
     8761 acatcttctt cgctgttaaa cagcgaacag ccgcttaaaa gggtaacgga aagcagtcct
     8821 ggcagcagta atttacgcaa ttgcatcggg tccctctcag atggacaaat tattaatttt
     8881 catctgcatc atttcgctca gtgccggagt aacatcgctt ttcacgcctg cttcccatgc
     8941 actacgcgca ccttgcttat cacctttgct cagcaatgct tcaccacgca ggtcggcaac
     9001 aatggcagcc cacccttcac ctttgatggt atcaagggtt ttcagcgcgg catcagcctg
     9061 cttgagctgt acctgaacgc gagcaagacg cagatttatc acggctttga gattttcatc
     9121 gctcgtgtct gccagcccct gttgtaactg ggcggcagct ttctctagtt catttttgtc
     9181 aacaaattgc tgcgcaagtt ccaaagaagc cagcgcacca taagtatttt tattttcagc
     9241 agcaaatttt tccgccgccg ggatgctatc cggtttgcct tcgctcactg cggtaaccgc
     9301 attttgatag gcaagagaag cggagcgtgc agaatcaacc tgatggctgt tccagtagcg
     9361 ccagccaatc agtgcgccaa cgcccaaaat caccccaaca gccagtgctt tgccattttc
     9421 agcaaaaaag cgtttaaccg cttctacctg gtcgttttcg ttctcgtaaa tttccacgct
     9481 gtccttctcc ttccttaacc cagtaacgtg cgcaaatgcg cggctacgct atcctgcgca
     9541 actgccgttt gctcaccaga gcgcaaatcc ttcactactg ctgtgccgtt agccacttca
     9601 gactcaccca gcaccacagc aacgcgggca ccccatttat cagcacgggc aaactgtttc
     9661 ttaaagttgc cgccgccgtg gttggtcatc aatttcacgc ccggtaattc atcacgcaga
     9721 cgctcagcta atgccatagc cgcagattgt gtatcagcac ctgaagccac caggtatata
     9781 tcgacaacag gatcggcttt aaattccgga ttaacggcct gtactaacaa tacaagacgt
     9841 tcgaggccca tcgcaaaacc gacagccggt gttgcacgac cgcccagttg ttccacaaga
     9901 ccgtcataac gaccgcctgc acacacggtg ccctgggagc cgagactgtt agtcacccac
     9961 tcgaaaacgg tacggttgta gtaatccaga ccacgcacca gacgctggtt tacggtgtaa
    10021 gcgatccccg cgctctccag cagtttgcac agaccggcaa aatgctcacg agattcctcg
    10081 tccagataat cacctaatgc cggagcgtcg ttgagaagcg cctgcacttc cggatttttt
    10141 gaatccagca cgcgcagcgg gttagtgtac atgcggcgtt tgcagtcttc gtccagcttt
    10201 tctttatgct gctcaaggaa tgccaccagc gcatcgcggt aattggcgcg tgcttccagc
    10261 gaaccgatag aattcagctc aagagttacg tgttcggaaa tacccagcgc gcgccaccag
    10321 cgggcggtga gcataatcag ttcagcgtcg atatccggac cttgcagacc gaaaacttcg
    10381 cagcccaact gatggaactg acgataacgc cctttctgcg gacgctcgtg acggaacatc
    10441 ggcccgatat accacagacg ctgttcctga ttgtacagaa gaccatgctc gatgccggcg
    10501 cgtacacagc ccgccgtccc ttcagggcgc agagtcaggc tgtcgccatt gcgatcctca
    10561 aaggtgtaca tctctttttc aaccacgtcg gtgacttcac caatcgcacg tttgaatagc
    10621 ggggtctgct ctacaatcgg caagcggatt tcactgtaac cgtagctgcc gagcacgttt
    10681 ttcagtgtgc cttcaatgcg ctgccaaatg gccgtttcgc caggcaggta atcgttcatg
    10741 ccgcgaatgg cttgaatgtt ttttgccacg tttattctct ttctgaatat aaaaatgaac
    10801 cctcaacgct tccctcaatg tttggggagc catgcgggtt caatcataca cgggaagcga
    10861 ggcgcttccc atcacgttat tatttttcga cctgctgaac gtcaattcga cgcgcttcgt
    10921 ccagctgact ggctttcgca cgaatgcgtg cttccagctg gtcgatcata tcgttgttgt
    10981 ccagacggtc tttgcgcacg ccatcttcat agaggccgct tttcttgttg ccgccggtga
    11041 cgccgagtgt agaaaccagc gcctcacctg ggccattcac cacgcagccg ataatcgaaa
    11101 cgtccatcgg agtgatgata tcttccaggc gttgctccag cgcgttaacc gtaccgataa
    11161 catcaaactc ctgacgcgaa caggtcggac aggcgatgaa gttgatacct cgcgaacgga
    11221 tacgcagcga tttcaaaata tcgaaaccga ctttgatctc ttcgaccgga tcggccgcca
    11281 gtgatacgcg cagcgtgtcg ccgatgcctt cagacagcag cagacctaag ccaatggcgg
    11341 attttactgc cccgctgcgc gcgccacccg cttcggtgat ccccagatgc agcggctgat
    11401 cgatttgttt tgccagcaaa cgataagact caacagcgag gaagacgtca gacgctttca
    11461 cgctgacttt gaactgatcg aagttcaggc gatcgagatg atcaacatgg cgcatggcgg
    11521 attccagcaa cgcctgcggc gtcggttcgc catacttttc ttgcagatct ttttccagcg
    11581 atccggcgtt aacgccaata cggatcggaa tgtttttatc gcgcgcacag tcaaccacca
    11641 tgcgaatacg ctcttcatta ccgatattgc cagggttaat acgcagacaa tcgacgccgt
    11701 attccgctac tttcagcgca atgcgatagt cgaagtggat gtcagccacc agcggcacgt
    11761 taacccgctg tttgatgagt ttgaacgctt ctgccgcgtc catcgtcggt acagatacac
    11821 ggacgatatc agcgccaacg cgttccagcg ccttgatttg actgaccgtt gcttcgacgt
    11881 ctgtcgtacg cgtattggtc atggactgta cggcgatggg agcaccatcg ccaatcggca
    11941 cattcccaac gtaaatacgt gttgattttc tacgttgaat tggagcctgg ttatgcatga
    12001 aaaatctccc gcgttacccg tctgttactg cgccggtgat tgttcggcat tgagggtcag
    12061 acgcgcaacc tggttagttc tgataaaacg actcagatcg acaggtttcc cttgatactg
    12121 gatctgtact gcggctggcg caccaatttt cagtttgtac ggtgcctggc cggttaagtt
    12181 caaattaccg tctttacgct gcataccgct aaacaatttt ttaccggtag catcagtgac
    12241 ctccagccag caatcggcag taaagttcat caccagcgca ttcggatcag ccgccggcgt
    12301 ggtcacgcca gcctgatcgg ttggcaacgg cgcagcacca tctggcgttg ttgttgccgt
    12361 tggtgccggg gtcgcggcgg tatcaacatt tgcctgcgaa ggcgagacaa ccgcattctg
    12421 ttgcggatca acagctggtg ctggcgcagt tacggcaggt gtttgcgtgt tggttgcggt
    12481 agtatccaca gacgctggcg gcgtggacgt tgtagccggg tctgtagttg tcgacgtatt
    12541 taacggaaca ctctgcccct gctcgctatt actgctcagt tccgccgaag attgatcggc
    12601 catagtggtg atctcttcct gctgagcttt gtggtcttgc caccaccagg caccgctcag
    12661 gccgataacc acaaacaaca ccagccaggt gaaggtcatc agccagccgt cgcgtttttt
    12721 gcggcgttta ccgagggaaa aactctgcat cggcgcaact tttgcagccc gtagtggagc
    12781 ctgcttttcc agccctggca gcagttcttc ttctggaata tgtaccagac gcgcataaga
    12841 gcggatatat ccgcgcagga atgttgaagc aagatcggcg ggtgccttat cttcttcaat
    12901 gtcgcgtacc gtggaaactt tcaggcaaag tcgctcggca acggcctgct gactaagtcc
    12961 tagttgttcg cgagcattac gcaggcgagc gccggtagta agtgcttcat tttggtcgtg
    13021 cgtggcttca gtattcattc gctgcaggta cgtttaaatg agaattgaga tgccggtgaa
    13081 ccatcatgct cacccacacc gcgaaacatc cgttaagtta accgttatca tacagtataa
    13141 gaccgtctgt gtgctcgtga caaagtccac attcactaag gctaacttac tgttgcatcg
    13201 ttacatactg ccttaaagtc agcaaaaacg caccgttaat attgaccaga caattgcaac
    13261 ttaatacttc attcataaaa cattacgcca cggtacataa agtaaccgtg gcgtaatggc
    13321 tatcagaccg ctttaatgtc gatggcttca ccctgcatcc gtttacgcag ggtacgtttc
    13381 gtacggtcga taacatcgcc cgccaactga ccacaggcag catcgatatc atcaccacgc
    13441 gttttacgca caatagtggt gaaaccgtag ctcatcagca cttttgagaa acggtcgata
    13501 cggctgttcg aactgcgtcc atacggtgcg cccgggaacg ggttccacgg gatcaggttg
    13561 atcttacacg gcgtatcttt cagcagttcc gccagttggt gagcgtgttc agtgccgtcg
    13621 ttaacgtggt caagcatcac gtattcaata gtgactcggc cttgattggc gttggatttc
    13681 tccagataac ggcgcaccgc agcaaggaac gtttcgatat tgtacttttt gttgatcggc
    13741 acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg aaattgccag cgcaacgtcg
    13801 atcatatcgc ccagtttatc cagcgctgga actacaccgg aagtggaaag cgtcacgcga
    13861 cgtttagaca ggccaaaacc gaaatcatca agcatgattt ccatcgccgg aacgacgttg
    13921 ttcaggttga gcagcggctc gcccatgccc atcatcacta cgttagtgat cgggcgctga
    13981 ccggtgactt ttgctgcgcc gacgattttc gccgcacgcc acacctggcc aataatttcc
    14041 gatacccgca ggttgcggtt aaagccctgc tgggcggtgg aacagaattt acactccagc
    14101 gcacacccca cctgcgaaga gacgcagagc gtagcgcggt cgtcttccgg gatatacacc
    14161 gtttcgacac gctggtcacc cacggcaatc gcccatttaa tggtgccgtc agatgaacgc
    14221 tgttcttcaa ccacttccgg tgcgcggatt tccgccacct ctttcagctt gccgcgcaac
    14281 actttgttga tgtcagtcat ctcatcaaaa ttgtcgcagc aatagtgata catccacttc
    14341 atcacctgat cggcgcggaa gggtttttca cctaaatctt taaaaaactc ccgcatctgc
    14401 tgacgattga gatccagcag gttgattttt ccatctttgg tggtgacgtt ttcaggtgtg
    14461 actaattgtt cagacatatg ctattccggc ctcgttatta cacgttatgg cccctggagg
    14521 gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg ggggcgctgc attgtacaaa
    14581 ttctggcgca cggatgccac gtttgcacgc ggcatttacg aaattattaa cgggtgcgcg
    14641 ggcacacttc gccttcgccg aagaaataag cgatttcgcg agcggcagat tcgacggagt
    14701 cagaaccgtg ggtaccattt tcggtcaggc tgtcagcgta atcagcgcgc agagtaccgg
    14761 ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg gtgacgctga acggcgtttt
    14821 caccttccag cacggaaacc acgatcgggc cagaggtcat gaattcaacc agaccatcaa
    14881 agaacggttt tccatcgtgt tcagcataaa agccacgtgc ctgttcaacg gtcaggtgca
    14941 gcattttggt gccgacaatt ttgaaccctg cagcttcaaa gcgcgcaaag atattaccaa
    15001 tgacgttttt tgctaccgcg ttcggtttga taatggaaaa agtacgttca atagccattt
    15061 ttacctctgt aaattgttct gttgttgtct gtaccagcgt acgagatggc gcggattata
    15121 atgagcaaca gggccgttga ctattgatga aggtaacatt tttttaaaat aaacctagtt
    15181 taagcaacaa tcagggacaa aacagactat tgcatgacaa atttcactgt cgcgatttgc
    15241 cctacgtcat ccatcaccag caattgataa tcacctttat ccgtcaaatg cagggtcacg
    15301 ttgcgcccgc gttcagttaa cggttcgcca ttcagaaacc accagcgttc acctgcccct
    15361 ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg gtaaacgttt aataatctcg
    15421 ccatcgcgaa cgcctgtcag ctgcagcggg agctgggcat cgtgaccgta tggcgggcaa
    15481 attgtcgagg ccggtggtaa gcgcacagcg cgacgttctg atgcgggtaa ccagggttcc
    15541 agtggtagcg gccagacatt tatcatttct tgtcgcgctt gcgggcaatc agcggcaaca
    15601 cgtttgccat tttcatccag ccagatgggg aaacgaatgc cattaatgcc ttcctgctcc
    15661 ggcagtaata gagttggcgg ctgactcccg tccagcagcc aggttgccag gcggcggcga
    15721 cagttaccgt caccttccgg caaagactgt ccgcccggcc agcagataac gccacgagtg
    15781 actgagttcg ggcgcgggtc ttccggcaga ttagcactgc gcgacagtaa gatattattg
    15841 acctgattca acaatggtac ggcgctggca aaaccaaact gaccaacaac gggcgtgccg
    15901 tccggtctgc cagtccagat cccaatgaca tagcgagcgt taaccccaat cgcccaggcg
    15961 tcacgatagc catagctggt gcccgttttc catgccagtg gggcgacgcg cggcaaggca
    16021 ccatccggca agggttgcgc ttcatcagcc ataatccggc gaatgatcca cgcagccccc
    16081 gacgacatta aaggccgttc aagcagcgga tcgtcaggct gtaagcgcaa tttgcctgcc
    16141 ttgccgtggc gagcaaacgc ggtatacgct gccgccatat cttccagttt tgcaccagcg
    16201 ccgccgagaa tgagtgaaag attcggcgca gcaccgttgg gcaaatataa cggcaatcca
    16261 acattgcgta actttgccgc aaaccgtttc ggtccatagg cttccagcac ctgcacagca
    16321 ggtaagttca gcgagcgcac cagcgcctcg ctcatgctga tcgggccatg aaaaccgcta
    16381 tcaaagttac ctggtcgata atcaccggtg cgccggggga cgtcttgcag cagtgatgcc
    16441 gggtggatca agccttcatc cagcgccaga ccataaacaa acggtttgag caccgatcct
    16501 ggcgatcgga tcgcattgac catatcaaca tgaccaaagc gtgaatcatc gttgagatct
    16561 accgatccca cccagccgcg aacacgcata tcggtatgat caaccacgat catcgccagt
    16621 gagctgcgcg gtggtaaacg tcctttccag ttttgcgcca gttcttccag acgtcgttga
    16681 agaccggcat ccagcgtagt aacgattttg tcgcttttgc ttttaccgag catcatgcgc
    16741 gaaaacagcg gtgcaagttg cggcatttgt cggggtgcca gccagatggg ttcttccctt
    16801 gactctttta cctgctcacg ggaccacaca ccttgcacgg ccatccgttc gagcacttta
    16861 ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc gcagtcggct gggcgcttgc
    16921 ggcaaaaccg ccagcattgc cgcctcggaa tagcttaaat tcgcaggcga ctttccgaga
    16981 taagcccaac ttgccgcacc gatcccctgc aacgtaccgc caaacggagc gcggttaaga
    17041 tacaaggtca gaatttcacg cttagacaga tgccattcca gttgcaacgc gcgccagagc
    17101 tggcgaattt tgccgccaaa tgttttgggg tgaggatcaa gcagacgagc aacctgcata
    17161 gtgagcgtgc tgccaccgga aataacccgt cccgaagtga gatcttgcca tgctgcgcgc
    17221 gccaccgaga atggattcac ccccggatgc ttccagaacc agcgatcttc ataattgatc
    17281 agcgcttcaa ggtaacgtgg agaaacatct tcgattgtta ccggataacg ccagatgccg
    17341 tcagcatcgg cgaagcgcca gagcggcgta ccatcctgcg ccacgaccac tcgtgcggga
    17401 ttgacttcat gcagcggtag aggccagagt ttatctgctc cccacgctgc aagaaaaagg
    17461 agaaatggcg cagctgccag cgttatccag cagccgcgtt tggttaacaa gcgaggcatt
    17521 tacggtctga caatcagcaa atcttcagcc gcgccggtcg cccgccattg gggaacatac
    17581 attgattcca ccatcggttg cggtacctga tacgttcccg gcgtcaccgc ccgcgccaga
    17641 tacaccagcg ttaccggttg gtattcatca acggcaaccg ccgccacaat gcgatcgtca
    17701 cggaactcaa tgtgcttaat actcgcctgc tgcatctggt tcagtaagtt ctgcacttcg
    17761 ccaccacttt gctccaggct ggcgctaccg ttcgccagat tctggttttc cagttccaga
    17821 cccgcaggca gcagatccac gactaacgca tccggcacgc tgttactggc ttttacctgc
    17881 aaccagacca gcaccagatc gccgctacgt aacgagtcca gcgatttgct cttaccatca
    17941 gtaccaagaa tatgacgctc aatttgcagc acattgttcg ccggtaaagg tgcggattgc
    18001 ggataaccgc tggcatccac acgcagccat aacggctgct cgccactgtt ggtcacctgc
    18061 aaggtggcaa gttgatcgct attcagattg ctgttttgcg ctttctcgcc tgtcagcggc
    18121 tcagttgaga aagaggtttg cgcctgccat ttacctggta aatcctgaat cgtacgggca
    18181 gccaggaaca acgcgttact ttcctgcgtc gatagcccgc gttcaccaaa cgcctgctgc
    18241 gaaagtgtgt tcagcaggga gtactgctca tcgggtagca gtttgttctc ttccagcaag
    18301 gagagcatca acgcgttgtc gcgcagtgga ctaccgtaat cacccagcca tatccgctca
    18361 tcactattac gcggcgtttt tagcgccagc gcaatcgctt cttcaccacg cgtcgcatca
    18421 cccatggttt tcagcgcaac gccaatttgc agcagcggta aaccagaagc ggcatctgca
    18481 cgatgctccc agatttcacg cagcgcaccc agcggagcct tttgctgacg ggccaacacc
    18541 agcgcagcgt atgactgtac ggcgaattta ctggctttga gattatccgc gtacgggatc
    18601 gacatcatgc ccggatcttg taaatagcgc agcagacgct cattaccccg gttaatggcg
    18661 tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca ggaaatccat cacgtaagcc
    18721 gtcagccagt actcttcgtc accgttttta tcccacagcg caaagccgcc gttatcacgt
    18781 tgcatttgca gcaaacggga aatgccgata tcgaccgatg cacggcgttt ctcatcactg
    18841 tcgcctttga tgcccaacgc ctgcagttgg gctgcgttgg tataaagtga cgggaacagg
    18901 ccgctggcgg tttgctcaag acagccgtac ggatacgctt ttaactcttt gatataacgt
    18961 gcgatgttca gcggtggttt gccgctcaac aacaattgcc cttccagcgt aacaggcgag
    19021 aagttttgca atccatccgc cggaatcgcc catgtctcac caggctgtaa cgccgtaccg
    19081 taattaaccg tttgtgccgg gaacgccgga cggacgccga ttttccactg cttatgctga
    19141 tcggcaacgg tttcacccgg taacgctaac ccgctaatgg tggcctgaat ttctccatcg
    19201 ccataacccg gcaatgctcg caccgggata aacagcgtag tacgcacacc tggcgctaat
    19261 tcaacggctg cgggtgaatc gctgaccagt tcaagcaaac cactggcggt cagggcaacg
    19321 ttcagttttt gcggtttatc ggtaagatta gtgatatcca gcgtcagacg cgaggtatcg
    19381 ccactcgcca taaagcgcgg catgttcagt tcagcaatca ccggtgcggc aactatcact
    19441 ttgctttcgt tgctaccgaa gtcatctgcc gtccaggctt gcgccatgac gcgcaattca
    19501 ccgttaaaat cgccaatcgg cagtgtaacc gagccttcgc cctgttcgtt gagcgttacc
    19561 ggcagcgcct gctgcgcgac aatattgacg tgattgaccg gcggtttacc accacgtttc
    19621 agctcatcac catcgccacc gaaacgcaga gctgccagac gcccctgacc ttcaataacc
    19681 tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct gaccaaagaa cgcctgccac
    19741 ggatctggcg tgacgtagtc agtaatattc agcacaccac tatcaacggc agacaccagc
    19801 acattcacct gtttaggctt ctcgccattt ttagtgctgg ctttaatttt cacggttaat
    19861 ggttgattgg gacgcatttt tgctggtgtt tccagcgcca gatcgaggcg acggttttca
    19921 tcaccaagcg gcagatgcaa cacaccaacc gcgcgttttg gcgtcgcgga gcgagattta
    19981 tcgccaggac gcaccaccag cgtactcaaa taaagatcat gacgattcca ggttttatcg
    20041 accggaatcg tcagatccag cccttgagcc ggaacatcaa tctcttgcca ccacaacggc
    20101 ccttcactgg actcgaccat cgcataacct ttacccgccg ttggcgcggc aatatgcaac
    20161 ttaatggtat cgccagggcg ataactggct ttatccagtt tcagcgtgac acggtcgggt
    20221 cgcacggcac cgctcccgtc gctgttgtcc tgccagctat agccagccca gaaacgaaca
    20281 ctactgaccg cttcattcgg cgctttgact tccagacgat aagcgcccca ctctaccgga
    20341 aaactgacct tgccagtttc gtccgctttc agatccagag tttgttcatt ttcaatcaga
    20401 tctttttgat caaactgtga ttgccagcct tcatcttctg accagtgcca gtagtaatcg
    20461 cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg cttttttcac gccttgcgca
    20521 tcgctataaa cgatgtcaaa agcggcgtta ctgccttcat caacaatggg ctgtttcacc
    20581 gtgctgtcag tgcgataatc gtaaaccgat ttcgaggcga actgcggacg gagccccggc
    20641 aatgcatcgg caggccagat agcctgctca gcgcggcgcg tcactgggcg accgcccgat
    20701 tccagcaaac taccctggaa aataacctgt aatggggaat gcgtttcctt ccactggctt
    20761 tctgtagaaa cttcgccgcg ccctttatca tccagcgtca actgaacttc atccagcgtg
    20821 cgggaaaggt tttcggcagc tatatcgccg aattcaaaac caggtaaggc tgacacagcc
    20881 tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat taccattagc aggcgcaccg
    20941 tacaggtagt atcccaccac ggagaatttt acttcatcat tcggcgtaag cggggttttc
    21001 tcaccggtca gattcagcgc catacgctct ggcataaaat cttcgacgtg gaaatcccac
    21061 atccgatact ggttatcgcc cgtgttagcg cgaatatgcc acataccggt tgccgcattg
    21121 ctatcgagtg gccaggtaaa gtggtagagg ccattttccg gctgactaac gacgctcctg
    21181 agtacctgcc catcgggttt aatcacgtct aacttgatgg gttgattggg caacgcttta
    21241 ccgtctgcat cacgcagcaa accattgagg atgaccgttt cacccgggcg ataaagatcg
    21301 cgcgggccaa acatgaaaaa ctgtttgcta tagcctggcg cgccagcaat gttaaattct
    21361 gctaagtcca gcgccggaag ttttaaatcg agtagcgttg tctgaccgtc tttacgtgcc
    21421 agcaataatg cggcgttttt atcattttcc agctgcacat gcccctgagc gtcactggtt
    21481 gcctgagtca gagtctgccc tttctcattt aataaagaga cttcaattcc ttgctgggcc
    21541 gcgccgtttt ccagactttg ggtaaagata tccagacgat tgtgataacg gtgagctgaa
    21601 acgccgatat cacttaacgt aaacagcgtc gcgggattac tgtaatcgta acgtccagcc
    21661 tgattcatca cagccagata cacgcccgcc tgctgaagcg gtttgatatc gcccagcggc
    21721 agcaataatt tttcacgggt gttacgcgca ggattgagat caaaccgtcc ggtgtagacc
    21781 agatccgcca tctgcagcag tttgtctgac tgccagttcg ccagcgaatt gcggtattcc
    21841 cattggctaa tgaatgctgg cagagattct ggcttaacgc ggaagaagtt aacatcgaca
    21901 ttattaacgt tgagcgccat taccggcagc ccttcaacga ctttaccagg cagcagcgaa
    21961 ccacggctgg caaaaccgac gctgggttgg atgtcgcggg tagttatagt tttttcgtaa
    22021 tctttactga aggttgcgtt gttgagtgct ttgacctcct tgccaatagt aacgatcaaa
    22081 tcacgtttcg gttcgaggtg gcgtaaacgc agctctttaa gattatctga cagctcccag
    22141 gcaccatcca ctttgccgct ttttttatcg acgacatgaa taacgcgtga gaaatcctga
    22201 tccgggtcga gagggatgga gaacgtcagc accagcgtgg ctgcaccatc aagttggact
    22261 tcagagagat ccagcaatgt cagcgcctta ccggcactct gttgtgccag tttttgtcgc
    22321 tccggcacgg agagttttgc tgaactcgcg ttttctgaag aggccgcttt agtaacttca
    22381 gaaggcgcat cttttttcac cgctgttggc gcgttatcgt tgttgtcgca ccctgccagc
    22441 gccagcatta gcatgcaggc ggctacgcgt aactttttca tttttcatcc ctgccacaat
    22501 ggcccgttag caacgtcgaa taattattat gcgtgagaat ttacgttatg taattcagta
    22561 tcacgcccat acaatccgaa ttttagtgag aattgtggct cagacagcca aatcagcctc
    22621 ttttgcttgt cgcttttcac taaacatccg acaatttagc ctaaccccgg caaaaatgga
    22681 gatgcctatg tccacgacat ggtttgtagg agccgactgg ctcgccgaac atattgatga
    22741 cccggaaatt cagattatcg atgcccgcat ggcgtcgcct ggacaggagg atcgtaacgt
    22801 tgctctggag tatctgaatg gacatattcc cggcgcagtg ttttttgata tcgaagcgct
    22861 ttctgatcac acttccccgc ttccgcacat gctgccgcgc ccggaaacgt tcgccgtggc
    22921 gatgcgtgaa ttaggcgtta accaggataa gcacctgatt gtctatgacg aaggtaatct
    22981 tttctcagcc ccacgagcat ggtggatgct gcgcaccttt ggtgtagaga aagtgtcgat
    23041 tctggggggt ggacttgcag gctggcagcg cgatgatctg ttgttagaag aaggtgcagt
    23101 agagctgccg gaaggagagt ttaacgccgc gtttaatcct gaagcagtgg tgaacgtaac
    23161 cgatgtgtta ttggcaagcc atgaaaatac ggcgcaaatt attgatgccc gcccggctgc
    23221 acgttttaac gcagaagttg atgaacctcg cccaggttta cgtcgcggac atattcccgg
    23281 tgcattgaac gttccgtgga cggaactggt gcgcgaaggc gaactaaaaa cgaccgatga
    23341 actggatgcg atattttttg gtcgcggcgt cagctatgac aaaccgatta tcgtcagctg
    23401 cggctctggt gtaacagcag ccgtggtttt gttggcactc gcgacgctgg atgtgccaaa
    23461 cgtgaaactg tacgacggcg catggagtga atggggcgcg cgggcagatt taccggttga
    23521 gccattgaaa taagcttttt acaggcaata aaaaaccgcc gaaaataaaa aaccgccgaa
    23581 tttggcggtt ttttattgct ggtctggttc gcggcctttc cagcaggttg acttgtgttg
    23641 ccggagcaac gcaggcaccc cacagtgttg aaatactctc ttttatgcct gactatgtca
    23701 agataataaa cagagtaatt attcaaacat gagagtatta ttcctgacgg cccttttcgc
    23761 cgtctcgcaa acgggcgctg gctttaggaa aggatgttcc atgaccgtaa atgcaggcac
    23821 cccacagtgc ttgctatcgc ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc
    23881 atggaccgca caatgaagat acggtgcttt tgtatcgtac ttattgtttc tggtgcgctg
    23941 ttcaccgagg taaataataa ccggagtctc tccagcaaca atttactggt ggtcaacaac
    24001 cttcagaaca gcaggtaagc ccgaatgccg ccctttgggg cggcatattt tagattatcc
    24061 gattctgttt aaagtgatgc aaaaaaccac cccagcgacg ttcatagaat ggcgcaatat
    24121 gtcagtaata aaataacctg gcttattagt ccgaattcag acaaatataa acaaatcccg
    24181 ctcaatatta aaaatactaa ccggtaaaag atattactta aacatgtaaa ttcactttct
    24241 ttaagcaaca aaaaaccgcc aaaatcaggc ggttatttgt tgccggtttg gttcgcggcc
    24301 tctccagcag attgacttgt gttgcatgag caacgcaggt gcttcacagc agagcaatat
    24361 tcgccagcaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
    24421 atcgacaccg gccctttccg ccgtctcgca aacgggcgct ggctttagga aaggatgttc
    24481 cgtggccgta aatgcaggtg tttcacagcg cttgctatcg cggcaatatc gccagtggtg
    24541 ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga tacggtgctt ttgtatcgta
    24601 cttattgttt ctggtgcgct gttaaccgag gtaaataata accggagtct ctccggtaac
    24661 aatttactgg tgattaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttggg
    24721 gcggcatatt tatattattc gattttgctt aaagtcacgc aaaaaccccc cccagcgacg
    24781 ttcatagaat ggcgcaatat gttcggtaat aaagtggcta attcctttct cccctttttt
    24841 cacctgacaa atatcgattg gttcatcgcc aggtaatgta tctgtcgcta cacttcccgc
    24901 cgcctgaata atttcttcga tatcaccatc ggcttcaatg ccaataagta aattaggctg
    24961 tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc ttcactggct taatggtttt
    25021 aaataaggtg gtgagtgaat caatcatttg tgctggcggc tctgcgactt ccgataatat
    25081 cagcgattca ccgccttcca gtacttcctg actactcagc ggatttcctt cttcaccaat
    25141 caacaaactg atttcacgcg gcataaactc tttaccggtt ggcagtttgg cattaaggaa
    25201 gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg ggcattacga caaatgcctg
    25261 ttcgtcttca accgcctgtt gaagtgcttc taacgaggta aaaaaaggaa tgacgctggt
    25321 gccgtcttct ttttcccagt gctgtaaatc aagcgcgcta tcttcaacca cagcctcgcc
    25381 ctgcgccgcc gtaccaggca cccagacggt ggattccagt agagtacgga aaaaggccgg
    25441 gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct tcaagttcat tttttgtttc
    25501 ggacataaga accacaattc attcaacgtt cggggcacaa gtgcctgatg cgccatactt
    25561 atcaggccta caagggattc acaatttgtt gaatttgcaa tactttgtag gccggataag
    25621 gcgtttacgc cgcatccggc atattagttt acgccgttaa cagattagca atcgtgcgca
    25681 caccaagtcc cgtagcaccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg
    25741 agcagtcgat atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg
    25801 ctgccgtgct cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt
    25861 tagacggcag ctggctgcgg tggaattccg ccagcggcag acgccagaat ggttcgttct
    25921 cttgtgaggc actcgccagc aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt
    25981 gataatcatt acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca
    26041 tttccggttt ctgcgcactg gcatcaatca gaccatcggc aagcaccagg cgcccttcgg
    26101 catcagtgtt catcacttca acttttttac cgttgcgata agtgatgata tcgcccagct
    26161 tgaacgcatt gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt
    26221 tcagtccgcg cgtaatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt
    26281 ccgacttcat cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag
    26341 tgataccttt acctaccagg cacgcgaaca ctggcgcttc tttatcgcca gttgggttgt
    26401 aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg tccgacggtg tgcagcccca
    26461 tataaccttg ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg
    26521 cgacgttgct gatcagatca acagcacgct gtgccagttg cgatggtccc aattcttctg
    26581 ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct
    26641 ggcgctgggc atcgtccaga tccggccact ccactttacg cgtgcctttc ggggctttgt
    26701 aaccttgcca gaatgcccag cagcgatccg catcccagcc ttcaccgctt agctgaacat
    26761 gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg gatcagcccc agatcgtctg
    26821 ccccgttcaa atgcagagta atgccatcat tattaatgct gtaagttgct ttttctcccc
    26881 agcgcgcgtc ggcaggttgg gtagagaggg taattttcat cgcttctgtc attttagtta
    26941 tccttattcg tttaacagca aaacgggcta cccccaggca gcccgttcat tcactcggct
    27001 tcgtccagcc agactaacaa aatcgcttcg aggattttct cgttggatgc ctgcgggtcg
    27061 tcgtcgaaat cttccagatc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc
    27121 ggatcaagat cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcgttccac
    27181 ttaagtccca tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga
    27241 tttcgaccac taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct
    27301 ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagtgagt
    27361 caaaaccttc acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt
    27421 gttcaatctc gataccgtta cgcagcgctg cgtcgagaat ggtttcaccg ctattagctt
    27481 ccagaacagc gccatcaggg cagagatcct gatgaggcaa aataacaatc tttggcatat
    27541 taaacctcgt ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga
    27601 gcggcgaaat cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca
    27661 tcatcgccct gcgccacttc actcaggtga gcggcagcat cgtcaatgac ctgacgttct
    27721 gcggcgctta acagcgcggc atcagcaacc agcgcgccgt gcagactttc cagcacacgc
    27781 gccgcttcta ctttttgttc tgccagcatt cgggctttta cgtcctgctc ggcatagctc
    27841 attgagtctt tgatcatcga agcgatttcg ctatcggtca gaccgtaaga cggtttgacc
    27901 tgaatagacg cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg
    27961 gcatcgacct ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc cggaatacca
    28021 cgcagcgcaa aacgcgccag tgagcggcag tcctgcacca gttcgcgctc accctgcatt
    28081 acatggatag acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg agcgcgggcc
    28141 accggaatag tggtattacg cggaatcact ttctccacca ggccgcccat cgtttcgagg
    28201 cccagcgaca gtgggatcac atcaagcaac agcatttcgc tgtctggctt gttacccacc
    28261 agaatatccg cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatggaagtc
    28321 agcggtggac gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac gcgagtagaa
    28381 ccgcccacca tcaccacttc cagtacttcg tcagcctcaa cacccgcatc tttcagcgcg
    28441 cgacgacagg ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca
    28501 cggctgattt cgccctgcca gcccgcaacg ttaacggtca cggagtccgc atcgctcagc
    28561 gcgattttgg ctgcaatggc ggcatccagc agttcacgct gaacgcggtt atcgctacga
    28621 tcaggaatgc ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg
    28681 ccgagcgcgg aatcaccgcc ggttgccagc acttcaaaca cgccgcgact taagcgcaga
    28741 atggaaatat caaacgtccc gccaccgagg tcataaacgg cgatcacgcc ttcctgaccg
    28801 gaatccagcc cgtaggcgat agccgcagcg gtcggttcgt taagtaagcg caggacgtga
    28861 aggcccgcca gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc aaagtacgcc
    28921 ggaacggtga taactacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc
    28981 agtgctttga ggatgtccgc agaaacgcgc accgggttca gcagccccgc cgccgtttca
    29041 atcatcggca ggccgttttc gctggcctgg aattgataag gcagatgcgg atagcgttgc
    29101 tggatatcag ccagcgagcg tcccatcagg cgtttaacag aactaattgt gttggcggta
    29161 tcgagcgctg cattagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga
    29221 acaacagatg gcagcaggtg acggccttca tggtcggcta acgtttcggc ctgaccgctg
    29281 cgcactgtcg ccaccagcga gttggttgtg cccaggtcaa taccggccgc cagacgacgc
    29341 tgatgcggcg cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc
    29401 cagaaattaa aaatcgagca gtttttcttc gagttgttcg gcactgcttc gcagtttatc
    29461 gagaaaacgc agcttacgca cggtatccgc cgccgcgtcc caggtctcgt tgtctaactg
    29521 ttcaaccatc aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc
    29581 cagccgcgct tcatctttcg cctgttcgat ctcgtccagc tcttcgcgca gctccaactg
    29641 ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaagccgtg
    29701 caaagaaagc aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt
    29761 aatggttgca gattgctgta cggcggcgag ttgttccgcc tggcttccgc tggcgaattt
    29821 atcaggatga tactgacgtt gtagatcctg aaaacgcagg ctcagcgcct gggtatcgag
    29881 ttgataacgg gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg
    29941 atccgccgcg ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg
    30001 ggttatcggt atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttaacg
    30061 ttcgggttgg tgaatttaaa cccttcgttc aggccttctt ttacgaagtc cagctgcgta
    30121 ccgtccagaa attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac
    30181 acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacat
    30241 ccggaggttc tcacgcccag acgcaggcca aaccctttgc cgcggttagc cagaaaggta
    30301 tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta
    30361 ttttgcttca cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag
    30421 aatagaacag tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt
    30481 gatcgcctgc gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga
    30541 agcgatagcg gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc
    30601 gttgacttta atctgcaact tcatcacgtc gccacaggcc ggtgcaccca ccatgccgct
    30661 gccgacgttc tcgtcgttgt tgtcaaagga acccacgtta cgcggattct cgtaatggtc
    30721 gataactttt tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg
    30781 agcccattcg atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag
    30841 gtcacgcaga cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc
    30901 agtagtaaaa cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc
    30961 cagcgcgcgc agcacgtagg acggttcgag gcttgctgac gtacaggcgg aaccggaaga
    31021 aaccgcgagg tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac
    31081 gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc
    31141 tttgatgccg ttccacagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat
    31201 ctcttctttt gcgatgcgat aggcttcgcc cattccgacg atctggtgaa caggcagagt
    31261 gccggaacgc ataccgcgtt cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacgcg
    31321 cggtttacga cgcacataca gcgcaccgat acctttcggg ccatagattt tgtgaccgga
    31381 gaaagacatc aggtcaactt tcaactggct caggtcgata ggcagtttgc ccacgctctg
    31441 ggttgcatca acgtgataga taatgccacg agcacggcac atttcgccga tagccgcaat
    31501 atcctgtacc acgccgattt cgttattcac gtgcatgata gaaacgagga tggtgtcgtc
    31561 acgcatcgcc gcttcgagtt ctttcaggtc gataataccg ttacgctgcg gtgccaggta
    31621 ggtgacttcg aaaccttcgc gctccagctg acggcaggta tccagtaccg ctttgtgttc
    31681 ggttttgctg gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat
    31741 cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc
    31801 gaccagatcg gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa
    31861 acggtgagaa cgggaggccg ggttaccaaa ggttccgtcc atcgtcataa actgcatcat
    31921 tttctcggca acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa
    31981 tttcattgct ctataaactc cgtacatcac tcaatgcaag gaatcaggct accggctgga
    32041 tgtacgaccg tgtttacgga gtatttagca ctccggcctg attctgaatt ctttttatta
    32101 agcgcgtaac ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg
    32161 ctgacgacca gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt
    32221 gttgagaaaa ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt
    32281 atcgccgccc tggcaaccac ctttaccctg acaacgggta gcatctacag attcgtcaac
    32341 ggcgctaatc acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc
    32401 gcctggtcca cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc
    32461 cagataagaa agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc
    32521 ttcagagttg agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag
    32581 tctcatgtct tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg
    32641 gggtattgca tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt
    32701 gactgattta gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct
    32761 gttgtcggat gcggtgtgaa caccttatcc gacctacggt tctgttcact gtaggcctga
    32821 taagacgcgt taagcatcgc atcaggcaac ggctgtcgga tgcggcgtga acgccttatc
    32881 cgacctacgg ttctgtttac tgtaggcctg ataagacgcg taacgcgtcg catcaggcaa
    32941 cggctgttat tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg
    33001 caggatattc aactcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt
    33061 catcacctgc cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag
    33121 atgaccataa aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac
    33181 ctgctcgcca ttttcctgag ttgccagcca ggccatacgc acttcatagg caataacctg
    33241 aaccgccatt gccaggttca gcgaactgta ttccgggttt gccgcaatcg cgacatgata
    33301 atggcatttc tgcaactctt cattggtcaa gccgacgcgc tcacgaccaa acaccagcgc
    33361 caccggggta tttgccgctt cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat
    33421 cggccacggc agcgtgcggg aacgtgcgct ggtgcccacc accaggctac aaccggctaa
    33481 cgcttcgtcg agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccccgccgc
    33541 cagggcaatc gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa
    33601 tcccattgtt ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac
    33661 cagcacaatt cgaatatttt gcagcattgt ctttcttcgt ctaaagatta ttcgcgcatc
    33721 ttatcataaa acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt
    33781 ctttaacatc cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc
    33841 gcgcaaggcg ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag
    33901 ccagaaaggc agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat
    33961 cgacacgatt cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact
    34021 tgaaggtact gatcaggatg ttcaatggat tatcgatcca ctggatggca ctaccaactt
    34081 tatcaaacgt ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga
    34141 agttgctgtg gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg
    34201 cgcacagctg aacggctacc gtctgcgcgg cagcaccgct cgcgatctcg acggtactat
    34261 tctggcgacc ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt
    34321 cggcaaactg ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct
    34381 ggcttacgtc gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gcccgtggga
    34441 cttcgctgca ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg
    34501 tggtcataac tatatgctga ccggtaacat cgttgctggt aacccgcgtg ttgttaaagc
    34561 catgctggcg aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg
    34621 ggtgatatca ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc
    34681 tttactttct ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc
    34741 gcaaggaaag gttatggcac tgccagtgaa caaacgcgtt ctcaaaattc tgtttattct
    34801 ctttgttgtt gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta
    34861 tcctgacgat aaaatttacg gtcccgaccc ctggtcggcg gaatccgtcg aatttacggc
    34921 taaggacggt actcgtctgc aaggctggtt tatcccttct tcgacgggcc ctgctgacaa
    34981 cgccatcgca accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc
    35041 gctggtcagt tggttacccg agcgtaattt caacgttttt atgtttgatt atcgcgggtt
    35101 tggtaaatca aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat
    35161 caatgtggtg cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag
    35221 tattggcggg gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc
    35281 ggtgatcctc gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg
    35341 cagtggctac ttacttgatg agagttacag cggcgaaaat tacatcgcca gcgtcagccc
    35401 gatcccgctt ttactcattc acggtaaagc tgatcacgtt atcccatggc agcacagcga
    35461 aaagttgtat agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca
    35521 cattgatgct ttttccgatc gtcacggcga tgtttatcgc gaacagatgg tggactttat
    35581 ccttagcgcg ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa
    35641 aaccacctta agacatgcta atccactggt cagaacagtt taagatgaga aaaattctgt
    35701 gacgcttgcc aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt
    35761 agctattctt atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac
    35821 catctgtcct ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg
    35881 ggttgaccgc caccatggca accttcagcg agcttaatgg tgtggatgat gatattgcca
    35941 gtcttgatat cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga
    36001 ctggttatga cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat
    36061 ttcactggct acgacgtggt ttccgtctgt gtccgtcatt tattaccagc caattcaccc
    36121 ccgccctgaa gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca
    36181 atctacaagc gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaatcgcgcg
    36241 atattcattt cctgtgtctg tatctgcaat attgtttgct gcagcaccac gctggaatta
    36301 cgcctcagtt taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag
    36361 cgcaggaaat tggacgccac tggcagcgtc gggcgctcca gcctgtacca cctgatgagc
    36421 cactgtttat ggcactactt ttttccatgt tgcgggttcc cgatccattg cgggatgcgc
    36481 atcagcggga cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc
    36541 tgggaaatgt tcgtttttat gatgaacagg ggttatgcga tcagctttat acccacctcg
    36601 cccaggcgtt aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt
    36661 tcgccagact gtatccacgc ctggtacgca ccacccgcgc ggcgctggcc ggatttgaaa
    36721 gtgaatacgg cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg
    36781 cctggctaat gcaggaaaac gatctgcatg aaaaacagat tattctactg accgggaatg
    36841 atagcgagcg agaagcgcag attgagcagc agttacgcga attaacgtta ctgccgctca
    36901 acattaaaca tatgtcggta aaggcatttt tgcagacagg cgctccgcgt ggcgcggcac
    36961 tgattattgc tccttatacc atgccgttac cgctcttttc accaccgctg atctatacgg
    37021 acctgacact gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag
    37081 ggaacaactt tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat cacccagaac
    37141 acgccgtggc ccagatattg atacaggaaa ccggcgaaaa cggtcatgat agcgatactg
    37201 ccgcccatcg cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgct accctggcga
    37261 gcggcaatat agcgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt
    37321 tgcaccacta tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg
    37381 ccgcaaatcg ccgagatcaa cagcatatcg cgtgcactac aacggcggaa aagtttatta
    37441 ctcagtgcaa agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc
    37501 gaggccgagt agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgc
    37561 gccccctgca ataaacaaac gcaggccaga aagcgccagt tatggcgaac cagcgccaac
    37621 cacgctgacc aaccggtgct ctcctgttgg cggcttgccc cttgtggctg aatcgtcgga
    37681 cggatgagaa agccgagcag catggatgcc acgcccaacg tcaacagcac gaggatcacc
    37741 cgataatcaa acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc
    37801 gagccccaca gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc
    37861 agtgcatcgg tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc
    37921 atcagccacg ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tgtcagcagt
    37981 gccagcacgc gcaaggcgga aatcaggcga gaaggatcgc tgacgcgggg cgcgatgagc
    38041 aaactcccga gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc
    38101 gttaaaccaa tccctttaag ccagacgctc cagaaaggta gaaaaatgcc gtaactaaaa
    38161 aagtatgtga aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc
    38221 cgtcaggagg cgttagtctg gagttaatcg aatgttggtg caagttgaaa aataagaata
    38281 ttcgagcctg cttcccgatg tctgtaacgc cagttcacaa gcactcaaaa actgcgatcc
    38341 gtgacgcatg ttgcaaagat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc
    38401 acaatggcga tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt
    38461 aaagcaatgg aaggcacatt tccggcaatg ggacgaaaaa caacctgccc ggtattaaaa
    38521 ttattcatat aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc
    38581 accagaatat tcgttgccac ctggacgata tttggctggc tgttttcttg tgcaaaccac
    38641 gctttaacga tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg
    38701 ccatccagtt gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac
    38761 accaccacta atggttcgtc aaaaagctcc agataatcaa tctccgggct ataaacagga
    38821 tggcgcatta agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg
    38881 attaaactca ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact
    38941 tttggcagta aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc
    39001 tgaacaattt tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg
    39061 tcctggagaa aacattctcc cgccgccgtc aacgcgactt tacgcttatc cctcaccagt
    39121 aacggaacac cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag
    39181 gtatgcagtt tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag
    39241 cgtaaatgcc gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag
    39301 aaattaacta tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga
    39361 aggtcaatac atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca
    39421 tttaccaact gattgacacc caaaacggtc gggtcactcc gcgtatttat accgacccgg
    39481 acatttacca actggagctt gagcgtattt tcggtcgttg ctggctattt ctcgcccacg
    39541 aaagccagat cccaaaaccc ggtgatttct ttaacaccta catgggagaa gatgcagttg
    39601 tcgtggtgcg ccagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg
    39661 ccatgcgtgt gagttatgca gattgcggca acactcgcgc ctttacctgc ccgtatcacg
    39721 gctggtctta tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc
    39781 cacaagggtt gtgtaaatcc cactgggggc taaacgaagt tccttgtgtg gagagttata
    39841 aagggctgat ttttggcaac tgggatacca gcgcaccggg cctgcgtgat tacctgggtg
    39901 acattgcctg gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg
    39961 gcggcgtaca aaagtgggtg atcaactgca actggaaatt cccggcagag cagttcgcca
    40021 gtgaccagta tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag
    40081 atgatggcag cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg
    40141 ccaaagatgc gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa
    40201 aaccggatgc taatgtctgg gtcgatggcg cagtttcaag ctattaccgc gaaacctatg
    40261 ccgaagcaga acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata
    40321 ttttccccac gctttcatgg ctcaacggca ctgccacgct ccgcgtctgg catccgcgcg
    40381 gccctgatca agtcgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag
    40441 ttaaagccgc ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc
    40501 aggatgactc ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca
    40561 acagcaaact gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc
    40621 ctggcattac taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg
    40681 ccgatcttct gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc
    40741 aggaggtgat gaaatgagtg cgcaagtttc actagagtta catcaccgca ttagccagtt
    40801 tctctttcac gaagccagct tactggacga ctggaaattt cgtgactggc tggcgcagct
    40861 cgacgaagag attcgttaca ccatgcgcac cacagttaac gcgcaaacac gcgaccgccg
    40921 caaaggcgtc cagccaccga caacctggat ttttaatgac accaaagacc agctggagcg
    40981 gcgaatcgcc cgtctggaaa cgggcatggc atgggcagaa gagccgccgt cacgcacccg
    41041 ccacttaatc agcaactgcc agataagcga aaccgacatc ccaaacgtat ttgctgttcg
    41101 ggtgaattat ctgctttatc gggcacaaaa agagcgcgat gaaacgttct atgtcgggac
    41161 tcgttttgac aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat
    41221 cgtcctggat caagcggtaa tcacttccca taacctgagt gtactgttct gatgaatcga
    41281 atttatgcgt gtcccgttgc ggatgtgccg gagggtgagg ctctccggat cgatacctcg
    41341 cccgtcatcg ccctgttcaa cgttggcggc gagttttatg ccattaacga tcgttgcagc
    41401 catggtaatg cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg
    41461 ctacacgccg ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat
    41521 ccgctcacca cttatccagt acacgttgaa ggtggtgaca ttttcatcga cttaccggag
    41581 gcgcagccat gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat
    41641 tagggctggc gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac
    41701 tgtcggcggc gaaagtcgcc agtctgcgtc agcgatttgg tgaacatatt ctggcggtgg
    41761 aaggcaacgt gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgactcgtt
    41821 ccggcaagct ggattgtttt atcggcaatg cgggcatctg ggatcacaat gcctcactgg
    41881 ttaatactcc cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac
    41941 tcggttacct gctgggtgca aaagcctgcg ctccggcgtt aatcgccggt gaaggcagca
    42001 tgattttcac actatcaaat gccgcctggt atcccggcgg cggtggcccg ctgtacaccg
    42061 ccagtaaaca tgccgcaacc gggcttattc gccaactggc ttatgaactg gctccgaaag
    42121 tacgggtgaa tggcgtcggc ccttgtggta tggccagcga cctgcgcggc ccacaggcgc
    42181 tcgggcaaag tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt
    42241 taccgctgca atttttcccg caaccggctg attttacggg tccgtatgtg atgttggcat
    42301 cacggcgcaa taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga
    42361 ttcgcggcat tcgccacgta gcggctgggc tggatcttta aggaagtacg atgaaagaaa
    42421 aaacgatcat tattgtcggt ggtgggcaag cggcggcaat ggctgcggcc tcgctacgcc
    42481 agcaagggtt caccggtgag gtgcatctgt tttccgatga gcaacatctt ccttatgaac
    42541 gccccccgct ctcgaaattc atgttgctgg aagattcccc acagttgcag tctgtgttac
    42601 ccgctcactg gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacat
    42661 tgggccgcga cacacgagag ttagtgttag ccaacggcga aagctggcac tgggatcagc
    42721 tttttatagc aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac
    42781 gctgctttac cctgcgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg
    42841 aacggtcagt cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcacca
    42901 cgcagcacag atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg
    42961 caccaccgcc cgtgcaacgc tatcttttac agcgccatca acaggctggt gtgcgcattc
    43021 tgctcaataa tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa
    43081 gtggcgagac gcttcaggcc gatgtggtga tttacggtat tggtatcagc gccaacgacc
    43141 aactggctcg cgaggccaac cttgatacta ccaatggcat tgtcattgat gaggcttgcc
    43201 gcacctgcga tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg
    43261 gtgcactaca ccgctgcgaa agctgggaaa acgccaataa ccatgcgcaa attgccgctg
    43321 ccgcaatgtt agggctaccg cttccgctac tgccgccgcc gtggttctgg agtgatcagt
    43381 acagtgataa cttacagttt attggcgata tgcgtggcga tgactggctt tgtcgtggca
    43441 acccggaaac ccagaaagcg atttggttta atctgcaaaa cggcgtgctt atcggtgcgg
    43501 taacgctgaa tcagggacgt gagattcgct caatccgcaa atggatccag agcggcaaaa
    43561 cgtttgatgc gaaacagctg acagatgaga acatcgcgct taaatcactg taaccaggat
    43621 aattagcgaa tatctcaacg cctggggcgt ggcgaggtac cagagtgtgt attacgttta
    43681 aatcacatta tcttgcaaag ggattggtta tgaactcact acgttatttt gattttggtg
    43741 ctgcccgccc cgttttgtta ttaattgccc gtatcgccgt ggtcctaatt ttcattattt
    43801 ttggttttcc caaaatgatg ggctttgacg gtacggtcca atatatggcc tcgctgggcg
    43861 caccgatgcc gatgctggca gcgattattg cggtagttat ggaagtgccc gccgcgatat
    43921 taatcgtgct tggctttttc acccgtccgc tggcggtact gtttattttc tacacgctgg
    43981 gtacggcggt gattggtcac cattactggg atatgaccgg cgatgccgtc gggccaaata
    44041 tgattaattt ctggaagaat gtcagtatcg ctggcgcgtt cttgctattg gcaattaccg
    44101 ggccgggggc aatttctctc gatcgccgtt aggaaagatg ccggatgcgg cgtgaacgcc
    44161 ttatccggca ttttataaat tacagccact ctacccgcaa cgacatctct gaggttgttg
    44221 attcccccgg cgcaagcgca atgaggtccc ccccttccgg gcgatgatgg tcatccggcg
    44281 catggctcat cggctccaga cagaagaaat caaacgcata tcctttatca aacgccgggt
    44341 cggaaacaaa gatgaaataa cacggtgcag gtggcgtcgt ttctatgatg atggcataac
    44401 cctcctgcgg ctgctcgatg cgagcctgac cattccatcc ggcaaaacca ttgttcaccc
    44461 actggcgcgg caacggcgcg agctggttaa aatccaattc ttgcggtagc tgctcgcaaa
    44521 actcacccgc cagccactgc tcccgctcca gccagtaacc gctcgcctgt gcctgaatcc
    44581 gcgtttgcgg cgacaacgga aaataaggat gccagccggt accaaatggc agcgtctctg
    44641 ccccttgatt ggtgacagag agcgtcaccg tcagcgtatc cgccgttaaa tgaaacgcct
    44701 gacttacccg atagtgataa acaccgctgc gatgttcata caccagacac aaactatcat
    44761 cgctgcacga ggcacattgc cactgcccca gccagccgtc gccgtgcaga tagtgcgcat
    44821 cccattcaac attcggttgc agttgatact cacgcccctg ccagacaaaa tggttgccgc
    44881 tcacccggtt tgcaaatggc accagcggaa aacaagaggc atcggttgcc acaccgcttt
    44941 ttttaccagg acgtaagagc ggcgtcgtat cgcgccagaa accttctatg acaccgccct
    45001 ggtcagaaac gtccagcgtg agccccccgt gagataaggt atagatggtc atccgctgac
    45061 tccttaatcc gggaagttaa tcacaacttt tccgcatttg ccgctcgcca tcagcgcata
    45121 ggcatcacct gcctgttcca gcgagaagcg atgggtaatg gcgttacgcg gccacagctt
    45181 ccagtcagtc aaatcgtggg cgcatttttc catatggaac agactggtca cccaggagcc
    45241 gataatccgc cgttgatggt gcatcagatc ggcgctgacc tcgaattcca cttttccggt
    45301 ttcaccaatg taaaccaccc gcccccagtc agcagtggat tgcagtgcca gcaagcgacc
    45361 tgcggcatta ccagaacaat cgagcgcaac atccgcgcca ccgtgggtaa gttcagcaat
    45421 tatctgcggc agaccttcgg tggtcgctaa atagccgtga tccatcaccc ctaactgttt
    45481 tgccatcgcc agacgttccg gcagcatatc aacgccgatg atccgttttg caccgcgacc
    45541 tttcgccagc atcatcgcca tcatgccgac cggccccaga ccgaccacca gcacgttatc
    45601 gctgccggaa acttcgccgc gcaaaatccc ttcataggcc gtgccaacgc cgcaactgat
    45661 aaacgcacca tcttcgtagc tcagcgcatc cggcaggagg atcagatctt tttcttccgc
    45721 cagcagatat tcggcatgac cgccgtcacg ctgccagccg taagccgctt ttcctttgcc
    45781 agtacaagag ataggaaagc cgcggcggca gttcgggcaa aaaccacagc cagaaatgtg
    45841 atacaccagc acgcgatcac cctctttaaa atggcgacag ccttgcccca tcgccacaat
    45901 ttgcccgcac ggctcatgac cgttgataaa gccctggtat aacggtttat cgggcgccgc
    45961 cgccgtggcg cggtgttgat ggtagatata gtggacatcg cttccgcaaa tcccggagga
    46021 tttcattttg atcagtacct ggttaatccc cggcgtcggc accgcaactt cccgcagatc
    46081 gacggtcgaa tttcctggta aataagctgc cagcatcgtt ttcataaatc cctcattaac
    46141 aatacgatta attttcatcc ctgctgcacc cgcgccaggg gcgttaaggt ttagcgtttc
    46201 gctttactgc tacgctgggt cagcaagata ttcgccagca ccgccaccac gatgatgacg
    46261 ccgcgcacca cctgctggaa aaaggagtta atcccgagca gcaccagacc gttaccgatt
    46321 agcgtaatca ccagtacgcc aagcagcgta ccgaacaacg agccgcgacc accggaaagc
    46381 gccgtaccgc caaccacgac tgcggcgatg acgtcaaact ccagaccgtt tgcggcacct
    46441 gcgttaccag aaccgaggcg cgccgccaac aaaatgccgg tcaccgccgc taataatccc
    46501 gaaagggtaa agataagaat gcgtacccga cgaacgttaa taccgcacaa ctgcgccgcc
    46561 gtggcattac cgccaacggc aaaaaccgag cgcccgaagg cggttttgcg gctgatgaac
    46621 acaaacagcg caaacaacac catcatgatc agcgcggata ctggcacacc tagaaattgt
    46681 ccgcccagcc agtccagcac ctcgttttcg tcaatcggca ctggcagggc gttggtcata
    46741 aacaagccca ttccgcgcag ggcgctccac agccccagcg tggccacgaa acttggcacg
    46801 ttaaacacgc cacgcagcac cccggcgagc gttcccatca gcgcgcctaa cagcaacacc
    46861 agcagacaag caatcgccaa cggaacttca aattgcagca aaaatgccag gcacaccgag
    46921 acaaaagcca ccatcggccc aacgctgaca tcaatttcac cggagataat aatcagcgtc
    46981 atcgcccagg cggcaatccc aatggtggcg gcatcgcgca gcacgttcat ctggttattc
    47041 aatgagataa agccaggcgc gttcagggag aagcccagat aaagaatggc aatcaccacc
    47101 agcaaaccga tctcattaat atggcgactg acaaattgtt tgagcgagac gctcttgccc
    47161 tgcggcaatg gtaatgacga agcagacatc ttaggttcct cggtaaaatt catcagtgca
    47221 cagacagaat ggcggacatc agctcatcca cattgaccgg agcgtgaaac tcctgcgaga
    47281 acgtaccgtg ctgtaacaac agaatgcggt cgcacaccag cggtaactcc tccacttcac
    47341 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    47401 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    47461 cggcatagac ccaacgaccg atcactactt tttgctgatt gccgccagaa agcgtgccga
    47521 tgggcgtttc gctactggcg gccttgaccg tcatccgctg catcacttct tcggtcaggc
    47581 ggcggatggt ggaccattgc agcactccgt tggcgctgat tttttgccga ttggtcagca
    47641 ctgtattttc gtcaacgccc aaccagggaa tgatccccgc ttctttgcgg ttttctggcg
    47701 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcgtgatt ttctcgccgt
    47761 tgataacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    47821 tgcgccctgc ccccagcaga ccagcaatgc cgagcacttc gccacgacgt agcgtaaaac
    47881 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagcacg gcctgatcca
    47941 caatttcctg aggtgcaacc ggcgcaatat caacgtgatc gcgccctagc attagcgaca
    48001 caatatgatg cgtggaagtg ttttcgagca tcacatcgcc agccacctgc ccgtcgcgca
    48061 taacggtggc acaggaggca atgcggcgaa tttcttccat ccggtggctg acataaatca
    48121 ccgccacgcc cagtgccgac atctttttca ccgcgctgat caccagttca acttccgcac
    48181 tggcaagcga actggtaggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    48241 gcgcaatttc caccagctgc ttatgcgccg ggcttagtgt tgaaacaagt tgttcaggac
    48301 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    48361 caatcatgcc gttgcggcgg ggccactgac cgaggcagag gttttccgcc accgtcagcc
    48421 cttccaccag acttaactcc tgataaaccg cgcgtacccc cagttcagcg gcacggcgag
    48481 tcagcgtagc ttcgtcacct tccagtcgcg tctcgccaat ccatatatcg ccgctatccg
    48541 gacgttcgct accggtaagc attcgaatga gggtcgattt tcccgcaccg tttttgccta
    48601 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    48661 cgggataacg cttattacct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    48721 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    48781 tgtttaccgt cttccgtttt ggtatagaga tcgataggca cctgaatcac tttttcaccg
    48841 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    48901 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    48961 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    49021 tttaccgcgc cgagtgtcgc accgcccgat tcccccataa tggcgttgag atccggcgtg
    49081 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctgatta
    49141 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    49201 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    49261 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagcggatcg
    49321 ccgaccagat acgccgagac atatttatcg accccctttt gattaataca ggtgttgtag
    49381 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    49441 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    49501 ctttctttcg aaatatcacc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    49561 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg agtatcaagg
    49621 tatattgcgc caatggtcat ttctttttcc gcagccctgg caaataaagc gctacctaat
    49681 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agagcctcct
    49741 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    49801 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    49861 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    49921 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    49981 ttttctttat gctgctgttg taaatcgcca tacagcgaca acagatcagg ttgcgagacg
    50041 gcaaagaaat ccgcttcgat actggttttc gccatctctt gtgcccactg tttcatttca
    50101 ctaaacagtt gctgtgcgat ttgttgttcg cccagcagtc gcagcgccat tccttgccag
    50161 aagagataat caaccggctg atcgttgtaa taactgtgaa tgttaatggt gcgatcgcca
    50221 gtcgccgcca gacgtaaaca acgcgtcgct tcagtttcat cgccctgcgc gttggcgcat
    50281 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    50341 ttctccggat aatgcagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    50401 tcaagatgct gccaagcgcg taataactgg ttgaggataa actgactggt gaccttccct
    50461 tccccgcctt cccacgggtg gaatttgcgc gtggcgagaa tgtccgccgc tttgtctgcc
    50521 tgacccgtga gatgccacaa attgagcaac tctgcagtca tgtcgtcgcg tttcagcgca
    50581 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    50641 tcaagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    50701 tcaagataac gcgcggccag ctcataatcg tgttgcttat tccacgcatg gatcgctaac
    50761 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    50821 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    50881 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    50941 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    51001 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    51061 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagggc gttaacgtcg
    51121 cgtccctgac acagaccgcg ccactgcgcg agagctgatt cgctacgacc atcgaaccag
    51181 ttcagccacc acagagtggc gttcagcgga taatcgcgca gcagtttctc gcgctgcaaa
    51241 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaggca aagcacttcc
    51301 tgattggttg ggcaggcgcg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    51361 ccattacgcg ccgccagtcg tgccagacca taatagcctc cggctttact gttgccgctc
    51421 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    51481 ctggcgcgaa tcaaactcgc ctgtccgcac tgcggatttt tgttcagccc atgtgcgcgt
    51541 ttcagagcct gagtggcata cgccaccgct tgcgggaaat ctgcgcggtt atactccagc
    51601 atcgccagcg ccagattaca gcggtaatcc agcggatcca gcgccacgcc gcgcaggtag
    51661 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    51721 gcttcatctg tactggtaat gtcttgtgct gacagtggcg ctttggcgac gtccggcaac
    51781 ggcaactctt gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    51841 agctcaatgg tcagcctttc aggattgata ccgtgcaaca cgccctggat ggcggtcgca
    51901 ggcatcagtg ccacggcatc atcaagtaac gcgttgcatt tgccgatttc gcggatcgcc
    51961 aggcggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    52021 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccaa agaatgataa
    52081 ggcaggaaat actgctcaaa acgcttctct tcgtaagcat caagccaggt aaaatcgggc
    52141 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt atcagttagg
    52201 ctcttatccc acgcctggcc aaattcactg tgtccccagc tccactgctt tttacctggc
    52261 gcaatatggt ggttggcaac gtgtagcaaa ccgccatctt catcgtgaca ccacgcgcca
    52321 acaaaatcgt actgtgattt ttcagccata tatgaggttg gaacgggcac atttttatag
    52381 cgagaaatgt ccactccagc ggagtagtcc actttgtagt aagtgccggt ggcgataggg
    52441 aaagcggaga cggcccgttt gccgtgatca aacaccgccg ttacatccgg cgggaagacg
    52501 ctctgatgcc cttccccccc tttcactgcc gggttggccc accacaagaa atgacgcggt
    52561 gtggcgttgc cgttatagac gcggctggcg atttccagcg ccgcccggtc agggcgcagg
    52621 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgcctac ccacaccgtc
    52681 tgtgcgccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    52741 tggtgttgcg gccagttaaa ctcaatccca ccggagatcc acggtcccag cagccccacc
    52801 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    52861 gcgcgatgca cccgaccgcc caattccggc aggatcatca ctttgatgta gtcgttttcc
    52921 agccacaccg cctgccagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    52981 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttcgaggaa catgggatgt
    53041 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    53101 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    53161 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    53221 tgaagatgag agcctgcatt aacaatcaac agattcgcca ccataacaaa tgcgtgattc
    53281 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    53341 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    53401 atatcgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    53461 agggtgactg gacgctgtgt ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    53521 atgcttgttt aagtccgaaa ggtgaatttg agtatttaca gattgatgca ccgacgccgc
    53581 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    53641 gcaccatcaa tctggcgctg gcaatccacg gtcaagttga tcctgtgacc ggagtgtcgc
    53701 aaaccatgcc gcaagcgccc tggacaacgc cggttgaggt gaagtatctg ctggaagaga
    53761 agctcggcat tcgggtgatg gtcgataatg actgcgtgat gctggcgctg gcggaaaaat
    53821 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    53881 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    53941 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggctgcctgg
    54001 aaaccgtcgc ctcgttaagc gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    54061 cggttaatac tcagcttgat cctgaaaaac tgactacagc gcagttaatc gctgcctggc
    54121 aaagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    54181 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    54241 gtgcctttgg tgagcactgg cttaatacca ttattcgcca gacaggattt aacccgttcg
    54301 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    54361 cacaacaggt gctggggatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    54421 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    54481 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    54541 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    54601 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    54661 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    54721 cagccagttc tttcgcttct gcttctttga agccgcgacg cgtaattgcc ggagtaccca
    54781 cgcgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    54841 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcga
    54901 ccagatcaac caggaacaga tggttatcag taccgccgga aaccactttg tagccgcgtt
    54961 ccaggaacac ttctaccatc gctttagcgt ttttagcgac ctgctgctgg taagttttga
    55021 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    55081 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    55141 cacctttcgc caggatcagg ccgccgcgcg gacccgccaa ggttttgtga gtggtggtag
    55201 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    55261 cgtgcgccat atcaacgaac aggtaagcac cgatgctgtc agcgatttca cgcattttcg
    55321 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    55381 ctttggcttg tttttccaga tcggcgtagt cgatatgacc ggtagcatcg ataccgtaag
    55441 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    55501 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    55561 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    55621 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    55681 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    55741 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    55801 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    55861 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    55921 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    55981 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    56041 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    56101 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    56161 agatacatca attaagatgc aaaaaaagga agaccatatg cttgacgctc aaaccatcgc
    56221 tacagtaaaa gccaccatcc ctttactggt ggaaacgggg ccaaagttaa ccgcccattt
    56281 ctacgaccgt atgtttactc ataacccaga actcaaagaa atttttaaca tgagtaacca
    56341 gcgtaatggc gatcaacgtg aagccctgtt taacgctatt gccgcctacg ccagtaatat
    56401 tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc gcgcagaagc acaccagctt
    56461 ccagatcaaa ccggaacagt acaacatcgt cggtgaacac ctattggcga ccctggacga
    56521 aatgttcagc ccgggccagg aagtgctgga cgcgtggggt aaagcctatg gcgtcctggc
    56581 taatgtattt atcaatcgcg aggcggaaat ctataacgaa aacgccagca aagccggtgg
    56641 ctgggaaggt actcgcgatt tccgcattgt ggctaaaaca cctcgcagcg cgcttatcac
    56701 cagcttcgaa ctggagccgg tcaacggtgg cgcagtggca gaataccgtc cggggcaata
    56761 tctcggcgtc tggctgaagc cggaaggttt cccgcatcag gaaattcgtc agtactcttt
    56821 gactcgcaaa ccggatggca aaggctatcg tattgcggtg aaacgcgaag agggtgggca
    56881 ggtatccaac tggttgcaca atcacgccaa tgttggcgat gtcgtgaaac tggtcgctcc
    56941 ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca gtgacgttaa tctctgccgg
    57001 tgttggtcaa acgccaatgc tggcaatgct cgacacgctg gcaaaagcag gccacacagc
    57061 acaagtgaac tggttccatg cggcagaaaa tggcgatgtt cacgcctttg ccgatgaagt
    57121 taaggaactg gggcagtcac tgccgcgctt taccgcgcac acctggtatc gtcagccgag
    57181 cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt ctgatggatt tgagcaaact
    57241 ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc tgcggcccgg ttggcttcat
    57301 gcagtttgcc gcgaaacagt tagtggatct gggcgtgaag caggaaaaca ttcattacga
    57361 atgctttggc ccgcataagg tgctgtgatt tgatgttgcc ggatggaaac atccggcaac
    57421 ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg gtacggatgc gaatgacccg
    57481 tgccacgtca aagacgaaga ttttaccgtc accgattttg ccggtttgcg cagtgcgaat
    57541 aatggtatcg acacaggtat cgacaatgtc gtccggtacg acaatctcaa ttttcacttt
    57601 cggcagaaaa tccaccatat actccgcgcc gcggtacagc tcggtatggc ctttctggcg
    57661 gccaaaacct ttcacctcag tgaccgtcat gccggtaatg ccgacttcag ccagtgcttc
    57721 gcggacatcg tccagcttga agggtttaat aatcgcatca atctttttca tgctattcct
    57781 tgaaaaggtc gcctgtcttt tgatctgcta aacgtaacac ataacgccaa ttcattcctt
    57841 gaaatcgttt gcatccagct cgtgtcggga aagcagttta taaaattctg tccggttgcg
    57901 ccccgccatt ctcgccgcat gggtgacgtt gcctttggta atttgcagca gcttacgcaa
    57961 atagttgagt tcaaactgat tacgtgcctc aacaaaggtt ggcagcgccg tgttttcgcc
    58021 ctccagcgcc tgctctacta atgcatcact aatcaccgga gatgaggtca gcgccacgca
    58081 ctgttcaatc acgttgacca actggcgcac attacccggc cagctcgcgg tcatcaggcg
    58141 tttcatcgca tcggtagaga acgcgcggac aaacggttta tgtcgctctg ccgcctggcg
    58201 caacaggtga tttgccagta gcggaatgtc ttctgtgcgt tccgccagtg ccggaatttt
    58261 caggctgaca acgttgaggc ggtaatagag atcttcacgg aattccccgc gcgccatcgc
    58321 ttttggcagg tcacggtgag tggcagaaat aatccgcacg tcgatatcaa tatcgcggtt
    58381 actgcccagc gggcgcactt tacgctcctg caacacgcgc aacagtttga cctgcaacgg
    58441 cgcaggcata tcgccaatct catcgagaaa tagcgttccg ccttccgccg cctggaataa
    58501 accttcgcga ttgctgacag cgccagtaaa cgcgccacgc gcatgaccaa acagctccga
    58561 ctccagcaat tgctcaggca atgcaccaca gttaatagca ataaatggtt tgctattgcg
    58621 cgggctggcg ttgtggatag cctgggcgaa aatctctttc ccggtgccgc tctggccatt
    58681 aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc tgttccagca aacgcagcat
    58741 cagcgggctg cgggtgacaa tcgcctcgcg ccagcgttcg tcggtagctg gcgcggattg
    58801 ttccagcgca tcatcaattg cctgatatag cgcgtcttta tcgacaggct tggtgaggaa
    58861 actaaaaacg ccctgctgtg ttgcagcaac ggcatcagga atagaaccat gcgcggtaag
    58921 aataattacc ggcattcccg gctgcacttt ctggatttca gcaaacagct gcataccgtc
    58981 catttcatcc atccgcaggt cgctgataac taaatctact ttttcgcgat tcagtacccg
    59041 taatccttca gcgccacttt ccgccgtgac cacactgtag ccttcgctgg tcaggcgcag
    59101 gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat aataaatgcg caggtttatg
    59161 gctcatcagg agtgacctca tgggtggatg gcgcgggctt ttcaccctcg tgcggcgtat
    59221 ccggcgagaa atttccagcc ggtttgcggg tcgagagctg gcgttcaata tcggtcaggt
    59281 tttccagctt gcgggtggtg agttccagct gctgttgtag aacgtgatgt tgctggcgca
    59341 aagtatccag ctcgctgtca ctggactgct ggagtttact gtagcgttgg cgctcttccg
    59401 ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg atacagtggg cgaacctgtg
    59461 ccgggatctc ggtacttaac gcttcaatac gcgcgaccag ctggcggcgc tcatacggcg
    59521 taattttggc gtcggcgagc aaaatccctt gtttaaaggt attttgccag ctgccgtcgt
    59581 catattggcg ggcttgctga cgcgactgcg caggcattaa acgatcagca caatccatcg
    59641 cccgcagcca gtaaagcgga ttggtttcag ttgatttacc ttgcagcgcc cagatgtcgc
    59701 tacattcagt agaaagatag tcagccagtt gataaaccgg aattttttct tctgctggcg
    59761 tatcaatggc tggcttattg tgactctgca cgcaacccag caatgccaga catggcagcc
    59821 ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat gtgtcgcata ttcaccagac
    59881 ttaaagccta tcccagtggg cgtaattgtt gcagacagtt tggacatgga cagcgcggag
    59941 aaaccggagc gtacacgtcg tacgtgagga tttcgagcac tgcccggggc cgaactggca
    60001 aataaaatag cctgatggga taggctgtta gtattatttc gtgtttttcg acgacggtaa
    60061 ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc agatacagtt ccccttgcat
    60121 acggcgaata caatccctgg caatgcttaa tccgagaccg ctgcccttca ccgccccttt
    60181 tcgctggtgg cttccctgaa aaaagggttc gaagatcatg gcgcgttcct cttgcggaat
    60241 gggcgtgcct gtattgataa catcaatata aacccgcgca ccatgtaaac tgctgcggag
    60301 gcaaatgtta ccggattcag ccccgtagtg caccgcattg gagtaaagat tatccagtac
    60361 gctcatcagc agcattggct ccgccaggca agctgttgct ttgagatcga cgtcggtatg
    60421 catcatttta gcccgtgcgg gcaggctatg agcagaaact actgtctcca ccagcggtgc
    60481 taactcaaca ttctccagtt ccaccgcacc atccgcctgt ttacggttgt aatcaagcag
    60541 ttgttcgatc agcttttgca aattacggct gctgctatca agaatgctca ccacctcttt
    60601 ttgctctggc gtaagcggcc cgacaacctg atcagccagt aattcagtgc cctcgcgcat
    60661 actcgccagt ggcgttttta attcatgaga taaatgtctt aaaaattgat ggcgttggga
    60721 ttccagccat gacaggcgct cacttaacca aagaatacgt tgcccaaccg agcgtaactc
    60781 gctcggtcca ctgaacgaaa cgctattgcc cagagaacgc ccttccccca accgattgat
    60841 catcctctcg atatttttca ccggaccaat aatcatacgc gtgaaaagca gtaccattac
    60901 cagactcacc agaaatagca ccagcgattg ccaaccaaaa tattgcccac gttcggcgat
    60961 ttcacgctga agttgctgcc cacgagagaa caccacggtg cgcgtggcct gcaccatttc
    61021 ggtattggca ctggcaaagg cttcaagacg cgcggcggca gcagcatcgg gaccactgtt
    61081 gttacactga agttgggcca gattgttcaa gtcctgacgc aatgcctggt aaagtttatc
    61141 gtcaggcagc acgcctgcgt gagcatcgag catttcgctg taacgcttgc gctggctttg
    61201 ataaaccttc gcaagcgtag ggtcgtccag cacgcaatac tgacggtagc tgcgctccat
    61261 ctccagtgcc gcgttggtca tcgcttcact gcgccgggca tcaataagcg tagtgcggtt
    61321 aaccagcgcc gcctgatcgc taagcgcatt caggctttgc caggcttgcc atgccagcac
    61381 caacaggggt agcagaatca gcaaaaatgc cagcattacc agttgtcgta atgagcgggg
    61441 aaaaacgggc cagcgtttca aggtgttact ctcgtcagac gcgaatagcc tgatgctaac
    61501 cgaggggaag ttcagataca acaaagccgg gaattacccg gctttgttat ggaataaggc
    61561 ggtgcctaac tcgacgtttc gcccgatggt tgatatagct acgctgatat cagaagttgg
    61621 acggcaggca ccttgttgtg cgtcattcga attttatgta gcacgtcccg aaggggctga
    61681 cataagtcgg tgaatgagcc actgcttact attatgcagt aactgtgcca ataaagaaaa
    61741 tagtttggta acgtactgat tatacgttgc tttgaggggt ttatggcttc tccactattt
    61801 gggtgtaaat cagccactgt gtcgctaaaa agagacagct taagataaac ttattatatt
    61861 tcagttaaat caatcagtta agtgtcgcca aaaagcgacg cgccaaacca cccattgtcg
    61921 tgatttacag acacaaaaaa gctcccggag ttgggagctt ataatagtgg ttggtgttta
    61981 atgccggatg cgggttgcaa aaccaatggg tttgacgact taccccaact gcttacgtgc
    62041 attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttcaggat gccaggagtt
    62101 gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc gaccgctttc
    62161 agtcgtgacc gccgtaatac cgttcggaga accgttcggg ttagccgggt aggtttcagt
    62221 gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt ccagtgccgc
    62281 cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag caatcggcat
    62341 ttgcgagccc accatcccct gcaacagcag cgacgggctt tgggtcactt caaccaggct
    62401 gaaacgcgct tcaaagcgat cggaggtatt gcgcacaaaa cgtggccaca actcactacc
    62461 tgggatcagt tcacgcagat tagacatcat ctggcaaccg ttacataccc ccagcgccag
    62521 cgtttgcgga cggtggaaga aggttgcaaa ctcatcgcgt acacggtcat tgaacaggat
    62581 agacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac caccgcacgc
    62641 gaccagggcg tggaaatcct ccaggcccgt gcgtccagcc agcaggtcgc tcatatgcac
    62701 gtcgatagca tcgaagcctg cacggtggaa agctgccgcc atttcaacat gcgagttcac
    62761 gccctgctca cgcagcacag caactttcgg acgtgcgcca gtggcaatat acggtgctgc
    62821 cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg cgtcgtttga
    62881 tttcgcctga tgctcctgat cggcacactc cgggttgtca cgcaggcgct gcatctgcca
    62941 ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata cagtctgccc
    63001 gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat ggacgcaatc
    63061 agcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg cacgaacctg
    63121 aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac ccagagtggc
    63181 tatatccgcg tcaatgccgc aatgaccagc aaaggccatt tccgccagcg ttaccagcag
    63241 gccgccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca gcgcctgaat
    63301 cgcgtcatag aagcctttca gttgcgcgac atcgcgtaca tctgccggtt tgtcgccaag
    63361 ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt tgcccaaatc
    63421 aatcagcagc agtgcgttat cttcggtaga aagctgcggc gtgatggtgt gacgtacatc
    63481 ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt cgcgctcttc
    63541 gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg ggatcgtcag
    63601 gcccagcgcc ggacaaagct cttcgcccac ggctttaacg gcttcataca ggcccgcatc
    63661 ttcacctggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc gtttgatatc
    63721 gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac gggcagaggc
    63781 ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt caccgtagta
    63841 gctgtcgagg ctggcggtag tgaccgcgca gttagcgact ggcacctgcc acggtcccac
    63901 catctgatcg cgcgccacca tgccggttac gctgcggtca ccgatggtca ccaggaaagt
    63961 tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa tggtgatccc
    64021 ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg tcatcttcgg
    64081 cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa aatgacgatc
    64141 gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg gtgcgcgctc
    64201 acgcttacac agttcgtcaa acagcggtaa ctgatcggca gcaaccgcca gcacgtagcg
    64261 ttcctgggat tcgttacacc agatttccag cgggctcatg ccaggttcgt cgctcagaat
    64321 atcgcgcagt tcgaatttac cgccgcgtcc gccgtcgctc accagctccg gcatcgcgtt
    64381 agaaagaccg cctgcaccaa cgtcgtggat aaacaggatt gggttggcat caccaagctg
    64441 ccagcaacgg tcgatcactt cctggcagcg acgctccatt tccgggttgt cgcgctggac
    64501 ggaagcaaag tcgaggtcgg catcagactg acctgacgcc atggaagacg ccgctccgcc
    64561 gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac cgacgttgat
    64621 ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca gcatgatcgg
    64681 tttgtgataa ccgcgcagct cttcgccgtt gtggctgttc actttttctt cataagtacg
    64741 gaagtagcca ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcgc cgcccagcgg
    64801 gccttcggtc atgatgtcca gcgcggtgac aatgcgctca ggcttaccga aatcttcttc
    64861 ccacggctgt tcgaagccag gaattcgcag gttggaaacg gagaaaccaa ccaaacccgc
    64921 tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc cggaaccggt
    64981 cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga ctttcatcag
    65041 aatatgcgcc ggttcctgat ggaaatcgta gcggcccgtt tcgtggtcag caaagtagcg
    65101 gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga gaacgtgatc
    65161 tggcgtggtt tcgaaagtat ttttgatcat cttgaacagc gatttcggct gctgttcacc
    65221 gtcgataatc cagtcggcat taaaaatttt gtggcggcag tgctcagagt tcgcctgagc
    65281 gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag catcctgcag
    65341 atagtcaatt tcatcttccg ccagagccag gccaagacgc aggttagcgt cgatcagcgc
    65401 ctgacggccc tgccccagca aatcaacgct ggtaaccgga gtcggttgat ggtgggcaaa
    65461 caactgctct gcatcatcta aagcaaaaaa gaccgtttcc atcatgcggt cgtgcagttc
    65521 agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat agtaagcaac
    65581 gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat cggtcgcttt
    65641 cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt tgccttgcgg
    65701 ggcgtggctg gcgagtgccg ggccatattt cagcaggcgt tcaagttgtg cgtgctcatc
    65761 atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa tattgtgaac
    65821 cgggagcctg gcagcctgaa aacgtgccag cagtttgtta attcggaatg ccgacagtgc
    65881 aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt cggtgtaccc
    65941 aaggggggga aacgggcgtc attataaaga atctgatgcg ctgacgaaac cgtttgcgtg
    66001 gaaataaaat caccatcgtg aattagcaac gcgtgccgcc aatggctgta ataagttgcc
    66061 atctggcgca ggtttacgca aaatgccgct catttatgag taaacctttc actattatta
    66121 cgttttttca agctgggacg cgcacgacac agagaattaa ctaattgaaa aaattaaaga
    66181 ttaattatct gttcatcggc attctggcac tgctgctcgc ggtcgctctc tggccatcca
    66241 ttccctggtt tggtaaagcc gacaaccgta tcgccgccat tcaagcgcgg ggagagttgc
    66301 gtgtgagcac cattcatact cccctgactt ataacgaaat caacgggaaa ccttttggcc
    66361 tggattacga actggcgaaa cagtttgccg attacctcgg tgtaaaactg aaagtgaccg
    66421 tgcggcagaa tatcagccag ctgtttgacg acctcgataa tggtaacgcc gacctgctgg
    66481 cggcaggact tgtctataac agtgagcggg taaaaaatta tcagcctggc cctacctatt
    66541 attccgtgtc acaacaactg gtttataaag tgggtcagta tcgcccacgt acgctgggca
    66601 acctgacggc ggagcaactc accgttgcac cgggtcatgt ggtggttaac gatctccaga
    66661 ccctgaaaga aacaaaattc ccggaattaa gctggaaggt agacgacaaa aaaggctctg
    66721 cggaattaat ggaagatgtc atcgaaggaa aactcgatta caccattgct gattctgtcg
    66781 ccatcagcct gtttcagcgc gttcacccgg agctcgccgt agcgctcgat atcaccgatg
    66841 aacaaccggt gacttggttt agcccgttag atggcgataa taccctttcc gccgccctgc
    66901 tcgacttctt caacgaaatg aatgaagacg gtacgctggc acgcattgaa gagaaatacc
    66961 tggggcatgg cgatgatttt gattacgtcg atacgcgcac atttttacgc gccgtcgatg
    67021 cggtactgcc gcagttaaag cccctgtttg agaaatacgc cgaagaaatt gactggcgtt
    67081 tgctggccgc tattgcttat caggaatcgc actgggatgc acaggccact tcaccgacgg
    67141 gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca aagcctcggc attacggatc
    67201 gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta tttgcaggat atgatgagta
    67261 aagtgccgga aagtgtgccg gagaacgagc ggatctggtt tgccctcgct gcgtacaata
    67321 tgggctatgc gcatatgctg gatgcccgcg ccctgacggc aaaaaccaaa gggaatcctg
    67381 acagttgggc tgacgtaaaa cagcgtctgc ctttacttag ccagaaaccc tattacagca
    67441 agctgactta cggctacgct cgtgggcatg aagcctacgc ttatgtcgaa aatattcgta
    67501 agtatcagat tagcctggtg ggttatctgc aagagaaaga gaagcaggct acagaagcgg
    67561 cgatgcaact ggcgcaggat tatccggcgg tatcgcctac ggagttgggc aaagagaaat
    67621 ttccttttct ctcgtttctt tcccagtcgt catcaaacta tttgacccat tctccctctc
    67681 tgctgttttc caggaaaggg agtgaagaga aacaaaatta atccgtcgag gattgcgctt
    67741 ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa gtcactgagc aacgccgcgc
    67801 actcatccgc cagtattcct tccgtaattt ccactcggtg attcataccc ggatgatgca
    67861 gcacatccat taaagatccc gcagcgccag ttttcgcgtc acgcgcacca aagaccacgc
    67921 gaccaatgcg actgtggatc atcgctccgg cacacattac acatggttca agcgtgacat
    67981 acaacgtggc gtcgatcaga cgataatttt gcatcaccag accgccctgc ctcagggcca
    68041 tgatttctgc atgtgcggtg ggatcatgtc gaccaatcgg acggttccag ccttcgccaa
    68101 taacacgatt gttatgcact aataccgcgc cgaccggtac ttcccgctca tcccaggcac
    68161 gcttcgccag cgtcattgca tgacgcatcc agtattcgtg gctaaattcg acttcagaca
    68221 aaaagaaaac tccggttata aaagcgcggc gcattataca cggacgctat gctttactct
    68281 attccagttg ctggagttca ccgcgcgggg ttactcgcca acgatgctga cagaaataaa
    68341 gcagcggatt gtcctgatta ctgtcgctat agccactgta cagccgcagc ggcgtgccga
    68401 ttttgcgctc cagttgtgcg accttttcat gtcccagaca acgcatcgtc aatacccaac
    68461 caccatagcc acgctgaatt tggctggcga taagattaac ccgcggcagc cagggcgtat
    68521 cgaaataaac cgcttcaacc agcggctgcg gagagccggt aatcaaccag atatcagcat
    68581 cggaacttaa caggtaggtg gttaatcgct cctgaaccag cggaaaggcg gtaacattgt
    68641 cacgaaacca gcgtacgaaa tcggcctgca acgcctgtaa acgtgcttcg ctgtgaccaa
    68701 aggtgcatcc ccacagaagc aggctcatcg gccagcgtgc cgcacgacct tttatcaata
    68761 acgcaatggc aatgaccggc aacaacggca ggacaagtaa cgcattcagc ggttggcgac
    68821 gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg atgcaatgtt ccatctaagt
    68881 caaaaaacac cacacgacgc tcgtgagttg ccaaaccata ctcctctgga ctgaaacatc
    68941 ctgattaatt ttcgctacta catagcctaa cagatagatc atcacttttc cggcaacaat
    69001 cgaccgcatc atggctaact ggaattttta attcatggca attagcggca atggaatata
    69061 aaattcactc gcgtgtgtct catattgcct gttgtcgcca ctatttcatc gcgaggaaag
    69121 atatgaacgg cttacttcgt atccgtcagc gttaccaggg gcttgcccag agtgataaaa
    69181 aactggcgga ttatctgctg ctacaacctg atacggcgcg ccatttaagc tctcagcaac
    69241 tggccaacga agccggagtc agtcagtcca gcgtcgtgaa gttcgcgcaa aaactcggct
    69301 ataaaggttt tccggcgctt aagttggcgt tgagtgaagc gctggcaagc cagccggaat
    69361 cgccctccgt gcccattcat aaccaaatcc gcggtgatga tccgttacgg ctggtcggcg
    69421 aaaaactgat taaagaaaat actgccgcta tgtacgcaac gctaaacgtt aatagtgaag
    69481 agaaactgca tgaatgcgta acaatgttgc gctctgcgcg gcggataatt ctgaccggta
    69541 ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa gctgatgaag attggcttca
    69601 atgctgccgc agtgcgcgat atgcatgcgc tactcgctac cgttcaggcg tcgtcccctg
    69661 acgatctgtt attagccatt tcttacaccg gtgtacgacg cgagttaaac ctggcggcag
    69721 atgagatgct gcgagtgggc ggaaaagtgc tggcgattac cggctttact cccaatgccc
    69781 tgcaacagcg cgcttctcat tgcctgtata ccattgccga agaacaagcg acaaacagtg
    69841 cttcaatctc tgcttgtcac gcgcagggaa tgttaacgga tttgctgttc attgcgctga
    69901 ttcagcagga tctggaactg gcaccagaac gtattcgtca tagtgaagcg ctggtgaaaa
    69961 aactggtctg agtaaagaat gcgcgtataa tgcccgcccg gtttgtgttg ttttgagagt
    70021 ttccttatgg cgctgttaat cactaaaaaa tgcatcaatt gtgatatgtg tgaacccgaa
    70081 tgcccgaatg aggcgatttc aatgggagat catatctatg agattaacag cgataagtgt
    70141 accgaatgcg tagggcacta cgagacacca acctgccaga aggtgtgccc gatccccaat
    70201 actattgtga aagatccgca acatgtcgag acagaagaac agttgtggga taaatttgtg
    70261 ctgatgcacc acgcggataa aatttaatat tctactctgg aagtagagta ttaattattt
    70321 tactgggaag ccttaacgcc attatattta tttaattgat gacattagca taatcattca
    70381 ctaagttaat ttatatagta tctgcccaga cacttattta tagttattaa aggcgcgtcc
    70441 gattggttca ccggacgcac cttaagtacg tttccttgtg ttataagaac agaaggatca
    70501 gctgtaaaac agcaatgatg attttgatga cccgtttaat caggtatcgg caatcagtca
    70561 ttcgtttttc cttaaacaag gaatgcagcc gttcagatta gcccttacat ctccccaaaa
    70621 ctgaacgtgc gagttattga gggtgcatgc tgcactccac accagagctt tgacgacacc
    70681 actcgtttca atgggggaat tctgtggcat ggtgtaaagc acagcaaaat cgccaataac
    70741 gaaaccaatt ttaatgtact tacgaattgg cggtcacgtt aatcttgcca tcaatatcgc
    70801 tttcttcgta aaggctcgag tttttatgat aaagattgca agttgcttgt aaaagataac
    70861 taccctgatc cataatcgct gttgttgagg gtgcgtgctg cactaaatta aagttacaaa
    70921 gtaaaacccc cgttccttac cagttcgggg gttttacttt ttaaagagaa cggtattatt
    70981 tttaactttc aataattacc gtggcgcaag catagtggcg ctcatcggcc agcgtcacat
    71041 gcatatttgc aacgcccagc ttttccgcca gttttaatgc ctcgccccac agtcgcagcc
    71101 gtggcttacc aagctcatcg ttaaatactt caaactgatt aaacgccaga ccattgcgga
    71161 tcccggtgcc aaacgctttt gctgcggctt cttttacggc aaagcgtttc gccagaaaac
    71221 gcaccggctg gtggtgcgtt ttccaaattt cccattcgtt atcgcttaat acgcggcgtg
    71281 ccaggcgctc accggatcgg gcgatcactg cttctatgcg agcgatctct acgatatccg
    71341 tgcctaaacc taatattgcc attagccacg cgcttccagc atcaggcgct tcatttctgc
    71401 caccgcatct ttcagtccgg tcatcactgc acgaccaata atggcatgac cgatattcag
    71461 ttcgtgcatc tcagggatgg cggcaatggc ttttacgttg tgataggtca gaccgtgtcc
    71521 ggcgttaact ttcaaaccga gactagcggc aaaggtcgcg gctttggcga tacgcgccag
    71581 ctcttgcgcc tgttcggcgt cagttttggc atcagcatag caaccggtgt ggatctcgat
    71641 aaacggtgcg ccaacctctg ccgcagcttt gatctgctct tcatcggcgt cgataaacag
    71701 agaaacctga atcccggcat ccgccagacg tttgcaggca tcgcgcattt tgtcacgctg
    71761 ccctgcaacg tccaggccgc cttcggttgt tacttcctga cgcttttctg gtaccaggca
    71821 gcaaaaatgc ggcttcgtct caaccgcgat cgccagcatc tcttcggtca ccgccatctc
    71881 cagattcatg cgggtatcca gcgtctgacg caggatgcgc acatcgcggt cagtaatgtg
    71941 gcggcgatct tcacgtaaat gcacggtaat gccgtccgcc cccgcctgct cggcaataaa
    72001 cgcggcctgc accggatccg ggtaagcggt accgcgcgcg ttgcgcagcg tagcgatatg
    72061 gtcaatgttg acgcctaaca gtaattcagc catgacaatc ctcatcattc ataatgtgtt
    72121 ttcaccgttc gcttaggcat aaattgccgg aacagttccc tgctctttaa aggtttaccg
    72181 ccaagatacg gcttaagcgc catgcgggta aagcgtttcg cggcgcgtag tgtgtctgcg
    72241 tcaggaaatt cccgtgcgtt taacgctttt aactgccttc cggtgaacgt tttattgtcg
    72301 ataacgacgc ttgcgataaa ccctttttct tcgcgataac gatacgtcat ggtgtcatct
    72361 accggctcgc cgctacccgc acaatgggta aaattgacgc cataacccag atgcccgagc
    72421 agtgccagtt caaagcggcg cagcgcgggt tctggcgtac cagtgacccc tgcaagagac
    72481 tgaatgcagt gcaagtaatc gaaaaagagt tcagagaagc gcgtctcgta ttccagtacg
    72541 cgggagagaa gttcgttgat gtacagaccg ctgtaaagcg tgataccgct taatggcagc
    72601 gccagcgaga cggcttcagc actgcgcagc gttttgactt cgccacgccc gccaaaacgt
    72661 agcaagagag gggtgaaagg ctgtaatgca cctttcaggg tagagcgttt agagcgtgcg
    72721 cctttggcaa ccagacgcac gcgacccgat tcctccgtga agacgtccag catcaggctg
    72781 gtttcgctcc acgggcgact atgcaggaca aatgcgcgct gccagccttc catcggagtt
    72841 actcttaaag atcgtcaacg taaccgagac tgcgcagtgc gcgttcgtcg tcggcccaac
    72901 cggatttcac ttttacccac agctcaaggt gaacaggcgc ttcaaacatt tcctgcatgt
    72961 ctttacgcgc ttcaatcccg atggttttga tcttggcccc tttgttgcca atgaccatct
    73021 tcttctgccc ttcacgctca acgagaatca aaccgttgat gtcataacca ccgcgttcgt
    73081 tagagacgaa acgttcgatc tccacggtca cggagtacgg cagttcagcg ccgaggaaac
    73141 gcatcagttt ttcgcggatg atttcagacg ccataaaacg ctgtgagcga tcggtgatgt
    73201 aatcttccgg gaagtgatga gtcgcttcag gtagatgctt acgcacgatt gccgcaatag
    73261 tgtcaacatt cagcccggtt tcggcagaga ttggcacgat atcgaggaag ttcatctggc
    73321 ttgccaggaa ctgcaggtgc ggcagcagat cggctttctc ctgcacgttg tccactttgt
    73381 tcaccgcgag gattaccggc gctttgcctt cgcgcagttt gttgagcacc atttcgtcgt
    73441 ccggcgtcca gcgggtgcct tcaacgacaa aaatcaccag ctcaacatcg ccaatagagc
    73501 tgctcgccgc tttgttcatc aggcggttaa tggcgcgttt ttcttccata tgcaggcccg
    73561 gtgtatcgac gtagatcgcc tgatacgcgc cttcagtatg gatccccaca atgcggtgac
    73621 gagttgtctg cgccttgcgg gaggtgatgg agattttctg ccccagcagt ttgttcaaca
    73681 atgtggattt gccaacgttc ggacgtccga cgatggcaat aaatccgcag taacttttat
    73741 cgatgctcat tccagctcca gttttttcaa cgcctgttcg gcggcagcct gctcagcctt
    73801 acgacggctt gaacctgtgc caaccaccgg ttcactcagg ccgctgacct ggcagtggat
    73861 tgtaaattcc tgatcgtgcg cttcgccacg gacctgaact accagataag tcggcagcgg
    73921 cagatgacga ccctgcaaat attcttgcaa gcgcgttttc ggatcttttt gtttatcgcc
    73981 tgggctaatt tcgtccagac gagtttgata ccagttgagg attaatttct cgacggtttg
    74041 aatatcactg tcgaggaata cgccaccaat taatgcttcg acggtgtcgg cgagaattga
    74101 ctcacgacga aatccaccgc ttttaagttc acctggtcct aaacgtaagc actcgcctaa
    74161 ctcaaattcg cgcgccagtt ccgccagcgt attgccacgg accagcgtgg cgcgcatccg
    74221 gctcatatcg ccttcatcca cacgagggaa acggtgataa agcgcattgg cgataacgta
    74281 gctcagaata gagtcgccta aaaattctaa acgctcgtta tgtttgctgc tggcactacg
    74341 atgagttaat gcctgctgca acagttcctg atgattaaaa gtgtagccca gcttccgttg
    74401 aagccgatta attacgatgg ggttcatgcg ataccaataa ataaatgcgt caacaattca
    74461 gcacacgaaa cagacctgat atacatggct ctgctaactg cttcgctgca gtttcagttt
    74521 agcggtatat ggggaccaac gctgtttcgt gtgccgtggc aacctggagg tgccaacctt
    74581 aaacttcggg ggaatattct atacacaacg acgggggatg tcgttagcca cgggagattt
    74641 atctcataaa taattcacgt tgtcgccata acggcgacaa cgtgaacgaa gatgagctat
    74701 taatggatgc cgccaatgcg acttaagcgc acacctgtcg gccattcgcc ttcttgctta
    74761 tcgaagctca tccagatagc cgttgcccga ccgaccagat tcgcttcagg cacaaagccc
    74821 cagtaacggc tgtccgcgct gttgtcgcgg ttgtcgccca tcatgaagta ttgtcccgga
    74881 ggaacaatcc aggttgccag ttgttgccct ggctgctggt aatacatccc cacctgatcc
    74941 tgcgcaatcg gcactgtcag aatgcggtgc gtcacatcac ccagtgtctc tttacgctcg
    75001 gaaagacgaa ttccattttc tttggtttcg tttttcggca cttcaaagaa tccgctggtc
    75061 gcttccccac cattacggcg tgagaaggtc tgaacgaaat cgctcggttc cacgtttgag
    75121 taggtgaccg gcagcgcgtt ttcacacgcc tggccggaac tgcatcccgg ttgaatcgtc
    75181 agctcttttg agaccggatc gtaagtgact ttatcgcccg gtaaacccac cgcgcgcttg
    75241 atgtaatcaa gctttggatc ttccggatat ttaaagacca cgatatcgcc gcgtttcgga
    75301 tgaccggttt cgatcagcgt tttctggtag ataggatctt taatgccata agcaaacttc
    75361 tctaccagaa taaaatcacc gattaacaga gtcggcatca tcgaacctga cgggatctgg
    75421 aacggttcat aaataaacga acgcacaatc aatacgatag ccagtaccgg aaaaacagaa
    75481 gcaccggttt ccagccagcc aggcttcggc gcaacctttt tcaacgttgc tttatccagt
    75541 gagtcaccgg cagccgcctg cgccgctgcc tgacgttccc gccgtttagg tgcgaaaaag
    75601 aatttatcca cgcaccataa aatgcccgtc accagtgtgg caatcaccag aatcagggca
    75661 aacatattcg ccatgccaac tcctaagggt tatttgttgt ctttgccgac gtgcagaatg
    75721 gcgaggaacg cttcctgcgg cagctcgacg ttaccgatct gcttcatgcg tttcttacct
    75781 tctttctgct tctgcagcag ctttttctta cggctgatat cgccgccata acatttagcc
    75841 agtacgtttt tacgcagctg tttcacggtg gatcgcgcaa taatgtgcgt accaatcgct
    75901 gcctgaatgg cgatatcaaa ctgctggcgt gggatcagat ctttcatctt ctccaccaac
    75961 tcgcgaccgc ggttttgcga attatcacgg tgggtgatca acgccagcgc atcaacacgt
    76021 tcaccgttga ttaatacgtc tacacgtacc atgtcggacg cctggaagcg tttgaagttg
    76081 taatccagag acgcataacc acgcgaggta gatttcaggc gatcgaagaa gtcgagcacc
    76141 acttccgcca tcgggatctc gtaggtcagc gccacctgat taccgtggta aaccatattg
    76201 gtctgcacgc cgcgtttttc tacgcacagc gtaataacgt tgccgagata tgcctgcggc
    76261 agcagcatgt gacactctgc aatcggctcg cgcagttcgt aaatgttatt taccgcaggc
    76321 agcttggatg ggctgtcgac gtagataact tcccttgacg tggtttcaac ttcatacact
    76381 acggtcggcg cagtggtgat cagatccaga tcgtattcac gttccagacg ctcctggatg
    76441 atctccatgt gcagcaggcc aaggaagccg cagcggaaac caaagcccag cgcgctggag
    76501 ctttccggct catagaacag tgaggcatcg ttcaggctga gtttacccag cgcgtcacgg
    76561 aaggcttcat agtcgtcgga acttaccggg aacagaccgg cgtatacctg cggtttgact
    76621 ttcttaaagc caggcagcgc cttttctgcc ggattacgcg ccagcgttaa ggtatcgccg
    76681 actggagcgc cgtggatatc tttaatcgca catacgagcc agcctacttc gccacatttc
    76741 agttcagtgc ggtcaacctg tttcggcgtg aagatgccca gacggtcggc gttataggtc
    76801 tgcccggtac tcatgacttt cactttgtcg cccttacgca gggtgccgtt tttaatacgg
    76861 ataagtgaaa caacgcccag gtagttgtcg aaccatgagt cgataattag tgcctgcaac
    76921 gggccttccg gatcgccttc cggcggcgga atgtcgcgca ccagacgttc gagaacgtcc
    76981 tgcacgccaa cgccggtttt cgctgaacag cgcaccgcgt cagtggcgtc gatgccgacg
    77041 atatcttcaa tttcttccgc cacgcgttca ggatcggctg ccggcaggtc aatcttgttc
    77101 agtaccggca ccacttcgag atccatttcc atggcggtgt agcagtttgc cagggtttgc
    77161 gcttccacgc cctgcccggc gtcgaccacc agcaatgcac cttcacaggc agccagcgaa
    77221 cgggaaactt cataggagaa gtctacgtgg cccggggtgt cgataaagtt aagctgatag
    77281 gtttcgccgt cagacgcttt gtagtccagc gtcacgcttt gcgctttgat agtaatgcca
    77341 cgctcacgct caagatccat ggaatcgaga acctgcgcct ccatttcacg gtcagacagg
    77401 ccaccgcaga tctggataat acggtcagac agcgtcgatt taccgtggtc aatgtgagct
    77461 atgatcgaaa agttacgtat attcttcata aagtatgatt attgtgcctt aatgcccggt
    77521 taaccaggct tttagaagtc gctgttctga gcttaacgtc tgtattaata gaaacgccgc
    77581 attctacact acaacattga ggcgaggaaa tgttcatacc gtatggattg tggtatctgg
    77641 aaacgtcctc gcatttgtta tgcaaaatgc aacaaagcca gtgaaatcac tggctcgcgt
    77701 cttccgaaga tgtttcaaat cgcacaaggc caggcggcaa tgccacgctt aatatgatcg
    77761 gttgccattc tgcccgggca gcaaacttac gcgagtagcc gcgagcaatc aggaacccgc
    77821 caataccgcc gaggaccgca ccacataatg ctgcgacgtc agaagcaaag agtagctgaa
    77881 atagcgaagc gatgaggagt aatcccacca gcggcgacat ataaaccagt aatgcggagc
    77941 taagcaggct gccttcggcg atccccaatt ccactttttg ccccggcact aacggctcat
    78001 cacagggtac gacaatggta tgcgtggttt gcgggccaag tttattcaac acgcggctac
    78061 cgcaaccggc gcgtgaagcg cagctgctgc atgaggcttt aacatcacaa ctgaccagcg
    78121 cctgcccgtt ttgccaggag acgacggtag cccactcttt gatcattgcg ctgccccgaa
    78181 cttaatattc tcggcaatgc gtttcgccgt ttgcggcggc agttcaccga caatggtgat
    78241 ttcggcgtta tcacgtacgc ttgtactgac ggttctgcgt ccggtgcgca acatctgatc
    78301 ggtgctcgat ggcgtagcgc ggttaacgtt taccgagaag ctgaataatc cgtcggaata
    78361 gagacgtgat tcgataggca tgttgtccat cgtcggtagc ggacgtcgac tactggaaac
    78421 ttcgctaaaa ccctgtggca accaggttgg cgtccagctg aatttagctt tttcacctac
    78481 aggaacagaa agcaacggcg gcaaatttgc cttcgccagc gtctgcatac tgctgctgat
    78541 atcctgattg acgttaaaag caatcacgcg aaattgttcc agcgtttcac catcgcgatc
    78601 aagaagatca acccgcattg gtaatttcga ttcggtgtcc atccacacga tgtagctgta
    78661 gcgtgtgcca tcacgagcaa ccacgcgaat aacttcacaa agacgatcag caatacgcgt
    78721 gcgcccgacg gagataaagt cgtagtaagg agaaaggcgt ttgaaatcgg tatagatgag
    78781 cgatggcaga gaatcaacaa tgtaatcgcc attaagagta aacggttcaa ggcccggttc
    78841 aaaatagctg atttcattgc cgcgctgtac cacttcccgg cgcgggccat ccatttgcaa
    78901 caattgtgca agaggacggt tatcgaggcg tgcatgtcga taacgcagag actcaacacc
    78961 ctgtttattg atgctgatga atgacagctc gtaattcagt gactgactgg ccaggttcat
    79021 ctgctgtaat aacgccccgg acgcgggagt ggccgaggcg ttagcagaga ataacaggct
    79081 acctgtcact aatgacatgg caaaccaaag ttgcttcatt actgcgattg cgttcctaaa
    79141 gtttgaattc ctggcacctg tacagcggct tgctgggttt gcgcctgctc aaactgaagc
    79201 tgttcagagt ggagtcggcg ttgcagttcg taatcctgca acattgcatt aatgcgacga
    79261 cgctgctcct gtacctgctg ctgttgacca ttatttgcgg tcgcttcaga aggtactccc
    79321 aggctaaccg ggctggcttt acccatcatc ggcagtgtat taaataccgg tgtttcgggc
    79381 tgctgggacg tttcagattg tccattatag tgctggacgc caacgataac tgcaagcgat
    79441 acgcatgcgg ctacgcccat ttgggtaagc tgtgccgccc acggacgtac tttctgccag
    79501 aatggcattt tctgccattg atgcggcgca ggctgggctt ccgggatcaa tgtcgccggt
    79561 tgacgtactg gctcttcttc aatggcggcc atcacgcgtg aagagatatc gaaatggagc
    79621 acctcgggag tatcaccccg cattgagtca cggattaagt gatagctttc ccaggttttc
    79681 tgcatttctg ggttatgagc cagttcgtta agcagctcac tatccagcgt ttcgccatcc
    79741 attaaagcgg aaagttgttc tttctgcatg cctaataccc ttatccagta tcccgctatc
    79801 gtcaacgcct gataagcggt tgaactttgt tatcaatagc ttccctcgct cggaagatac
    79861 gtgaacgcac cgtacctacc ggacaatcca tgatagcggc tatctcttca tagctcaggc
    79921 catccagctc ccgcaaggtt attgccatgc gtaaatcttc cgggagggac tcaatagttc
    79981 ggaaaactat ctgtctcagt tcttctgaca acattaagtt ctcagggttc gaaatttctt
    80041 tcaacgcgcc gccactttcg aagttttcag cttcaatggc atccacatca ctggaaggtg
    80101 gacgacgccc ctgagcaacc aggtaatttt tcgctgtatt tacagcaatc cgatacagcc
    80161 atgtataaaa agcgctatct ccccggaacg aatccagcgc acgataggct ttaataaaag
    80221 cttcttgtac cacatcagga acatcacccg acggcacata gcgggaaacc agactcgcca
    80281 ctttatgctg atagcgcact accagtaagt taaaggcttt ctgatctccc ttctggaccc
    80341 gttcaaccag gacctggtcc gttaactgct cgctcatccg aggtaaagtc tccccaaacc
    80401 aaatttccac gcgctatcga aacgccactc cattagctgc aatttgagca agcaaagggt
    80461 tagagtgtct cgtttttgta aagttccgta acgcatctgt ttttgtttgt catgctgtag
    80521 acggatcatt atctatcatt ataagtctac agaatctgaa catcgcatta tctgtgtaga
    80581 aatgcccatt taactgcctg aagagtaacc caacggcctt tttatttcac cacctaatcc
    80641 tccaccagcc agtaacttct ctttttctcg ccgccctgcg tcagcgtgtt tagccactgt
    80701 aacaaatatt aaaatagcag gtgtttattc gcacaacatg atgctatgct gaccaaaccg
    80761 tgtttagtaa attaaacaaa gaaaatgaat actctccctg aacattcatg tgacgtgttg
    80821 attatcggta gcggcgcagc cgggctttca ctggcgctac gcctggctga ccagcatcag
    80881 gtcatcgttc taagtaaagg cccggtaacg gaaggttcaa cattttatgc ccagggcggt
    80941 attgccgccg tgtttgatga aactgacagc attgactcgc atgtggaaga cacattgatt
    81001 gccggggctg gtatttgcga tcgccatgca gttgaatttg tcgccagcaa tgcacgatcc
    81061 tgtgtgcaat ggctaatcga ccagggggtg ttatttgata cccacattca accgaatggt
    81121 gaagaaagct accatctgac ccgtgaaggt ggccatagtc accgtcgtat tcttcatgcc
    81181 gccgacgcaa ctggtagaga agtagaaacc acgctggtga gcaaggcgct gaaccatccg
    81241 aatattcgcg tgctggagcg cagcaacgcg gttgatctga ttatttctga caaaattggc
    81301 ttgccgggca cgcgacgggt tgttggcgcg tgggtatgga accgaaataa agaaaaggtg
    81361 gaaacctgcc acgcaaaagc ggtggtgctg gcaaccggcg gtgcgtcgaa ggtttatcag
    81421 tacaccacca atccggatat ttcttctggc gatggcattg ctatggcgtg gcgcgcaggc
    81481 tgccgggttg ccaatctcga atttaatcag ttccacccta ccgcgctgta tcacccacag
    81541 gcacgcaatt tcctgttaac ggaagcactg cgcggcgaag gcgcttatct caagcgcccg
    81601 gatggcacgc gttttatgcc cgattttgat gtgcgcggcg aactggcccc gcgcgatatt
    81661 gtcgcccgcg ccattgacca tgaaatgaaa cgcctcggcg cggattgtat gttccttgat
    81721 atcagccata agcccgccga ttttattcgc cagcatttcc cgatgattta tgaaaaattg
    81781 ctcgggctgg ggattgatct cacacaagaa ccggtaccga ttgtgcctgc tgcacattat
    81841 acctgcagtg gtgtaatggt tgatgatcat gggcgtacgg acgtcgaggg tttgtatgcc
    81901 attggcgagg tgagttatac cggcttacac ggcgctaacc gcatggcctc gaattcattg
    81961 ctggagtgtc tggtctatgg ctggtcggcg gcagaagaca tcaccagacg tatgccttat
    82021 gcccacggcg tcagtacgtt accaccgtgg gatgaaagcc gcgttgagaa ccctgacgaa
    82081 cgggtagtaa ttcagcataa ctggcacgag ctacgtctgt ttatgtggga ttacgttggc
    82141 attgtgcgca caacgaagcg cctggaacgc gccctgcggc ggataaccat gctccaacaa
    82201 gaaatagacg aatattacgc ccatttccgc gtctcaaata atttgctgga gctgcgtaat
    82261 ctggtacagg ttgccgagtt gattgttcga tgtgcgatga tgcgcaagga gagtcgtggc
    82321 ctgcatttta cgctggatta cccggaactg ctcacgcatt ccggtccatc gatcctttct
    82381 cccggcaatc attacataaa cagataaaaa gcctgggtta gcgccgtata cgcttcggaa
    82441 tagttctggt ctggcccacg aatgactaag cgatcgctaa agcattctcc cgcctgcggg
    82501 gagaatgcca gcagcacccg atgcggcagt cgcgcttcgt tttccgccac atccgtccgc
    82561 aaacgtaaat gccagcccat acttaatgcc agctccgtaa aactattacc aatctgctct
    82621 ggcagcacca cgcagaaaaa tccctcttcg gtaatgcact ccgccgcaca ggtcagcagc
    82681 gatgggtgat caagcgtagt ggtatagcga gcctgttccc gttgaggtgt cgcgcactct
    82741 actccctgct gatagtaagg tgggttactg atgattaaat cgaagcgtac tgtctgctgt
    82801 gtgatccact gctgaatatc cgccgtatgg acgttaatcc gctctgccca cggggactgg
    82861 ttgatatttt cctgcgcctg agtggcagct tcactttcca gttcaactgc atcaatcatc
    82921 acgctgtcat cggttcgctg cgccagcatt aatgccagca acccgctacc agtgccgata
    82981 tcaaggcaac gttttacccc agcaaccggt gcccatgcgc ccagcaatat gccatccgtt
    83041 cccactttca tcgcacagcg atcatgagca acaaaaaact gtttaaaagt aaatccatta
    83101 cgacgaagca cggatgtaga ctgtgacatg aaaataaaac cttgcaggaa aaacggcgat
    83161 agcaccgggt gagaacaata cccgagaagc gatatccata caaacagatg aagattgcag
    83221 ccgtaacgtc tataatcagc gccccacaca gaggtagaac atgactgtaa cgactttttc
    83281 cgaacttgaa ctcgacgaaa gcctgctgga agccctccag gataaaggtt tcactcgccc
    83341 gaccgccatt caggctgccg ccattccgcc tgcgctcgat ggccgtgatg tactcggttc
    83401 tgcgccgaca ggcaccggta aaacggcggc gtatctgctg ccagcgttgc agcacctgct
    83461 cgatttcccg cgtaagaaat ccggtccgcc gcgtattttg atcctcaccc caactcgcga
    83521 gctggcgatg caggtggccg atcatgcccg cgaactggcg aaacatacgc atctggatat
    83581 cgccaccatc accggcggcg tagcctatat gaaccacgcg gaagtgttca gcgaaaatca
    83641 ggacatcgtg gtcgccacga ccggacgtct gctgcaatac ataaaagaag agaacttcga
    83701 ttgccgcgcg gttgaaacgc tgatcctcga cgaagcagac cgtatgctgg atatgggctt
    83761 cgctcaggat atcgaacata ttgctggcga aacgcgctgg cgtaaacaga ccctgctctt
    83821 ttcggcaact ctggaaggcg atgcgattca ggactttgcc gagcgtctgc tggaagatcc
    83881 ggtggaggtt tctgccaatc cgtccacccg tgagcgcaaa aaaattcatc agtggtatta
    83941 ccgcgccgat gatcttgagc ataaaaccgc gttgctggtg catctgttaa aacagccgga
    84001 agcgacccgc tcaattgtgt ttgtgcgtaa gcgtgagcgt gtgcatgagc tggcaaactg
    84061 gctgcgcgaa gcgggcatca acaactgcta tctcgaaggt gagatggtac aaggcaagcg
    84121 taacgaagcg atcaagcgtt tgaccgaggg tcgcgtaaac gtactggttg caaccgatgt
    84181 tgccgcgcgc ggtatcgaca ttcctgacgt cagccacgtc tttaacttcg atatgccgcg
    84241 cagtggcgat acttatttgc accgtatcgg acgtaccgcg cgcgccggtc gtaaaggcac
    84301 cgcaatttcg ctggtagaag cccatgacca tctgctgctg ggcaaagttg gtcgctatat
    84361 tgaagagcca attaaagctc gcgtgattga tgagttacgc ccgaaaacgc gtgcgccaag
    84421 cgagaagcag accggcaagc catcgaagaa agtactggca aaacgcgccg agaagaaaaa
    84481 agctaaagag aaagagaagc cgcgggtgaa aaaacgccat cgcgatacca aaaatattgg
    84541 taagcgccgt aaaccaagcg gaacgggcgt gccaccgcaa acgacagaag agtaatctca
    84601 atgccgggtt taagcccggc attattatta tcccggcaaa aaacactctt caacacactg
    84661 aataaaaatg tgcatcgccg ggctaaccgc ttttccagca tgatgtgcgc acattgccgt
    84721 aatggtctgc gactgttcgc caaaaggcaa ttcaattaat tctccgctct caagctcttt
    84781 ttccaccgca aagcggggca agtaactgac accgatattc gccgcgacac aacgtttgat
    84841 actttcaatg cttaacagct caatggtgtt ttccaccgtg ctccgccgct ggcgcaatgt
    84901 gctttcaaag atctgccgaa agacacattg cggttcgttg ataataaagc tacaggcgtt
    84961 atgtcttccc ggctcagtaa aatcgacatc tgcaatttgc ggtgaagcta ccagcaccag
    85021 cgattgctca cccaattctc gtcggttcag ggcatcatca ttccctacgc gataaaaaac
    85081 cccgacatcg gcctcatcat tcaatagcgc atcacggatc acgtaacagt tcagagactg
    85141 caacgataaa cgcacttttg gggcgcgttg ccgaaaacgc tgcaacacct gcggcattcg
    85201 gtaagagagc aacgtttcgc ccgaaacaac gcgtagttcc ccgtccggat ccgactcctt
    85261 tttggcagcc tcacgaaggg tatccatcac tctggtgagt tcataaatgt acggcagcag
    85321 ctttttccct tcgcgggtaa ggcacattcg tcgaccaatt ttctcaaata actggactga
    85381 gaactcctgc tcaagctgct gaatatgaaa agtcaccgtc gattgtgtgc agcacaattt
    85441 ttgcgaagct cgcaaaaagg aaccctcttc caccacggtt ttaagcgtaa taaaacggcg
    85501 cagatccata accccaaacc tatcgaaaat atcgaattta gaatgtaaaa acattcattt
    85561 ttttaaatgt tccgtgtcgg gtactgtcta ccaaaacaga ggagataaca agtgacaccg
    85621 acccttttaa gtgctttttg gacttacacc ctgattaccg ccatgacgcc aggaccgaac
    85681 aatattctcg cccttagctc agccacaacg cacggatttc gtcagagtac ccgcgtgctg
    85741 gcagggatga gtctgggatt tttaatcgtc atgttgctgt gtgcgggcat ttcgttttca
    85801 ctgactgtta tcgacccggc agcagtgcat ctgttgagtt gggcaggagc ggcatatatt
    85861 gtctggctgg cgtggaaaat cgctaccagt ccaacaaagg aagacgggct tcagacaaaa
    85921 ccaatcagtt tttgggccag ctttgctttg cagtttgtga acgtcaaaat cattttgtac
    85981 ggtgttacgg cactgtcgac atttgttctg ccgcaaacac aggcgttaag ctgggtagtt
    86041 ggcgtcagcg ttttgctggc gatgattggg acgtttggca atgtgtgctg ggcgctggcg
    86101 gggcatctgt ttcagcgatt gtttcgccag tatggtcgcc agttgaatat cgtgcttgca
    86161 ctgttgctgg tctattgcgc ggtacgcatt ttctattaac gaaaaaaagc ggaagaagtc
    86221 gccctcttcc gcttagtaac ttgctactta agccttacag gctttcagta aaggtacgag
    86281 caataacgtc gcgctgctgt tccggagtca gagagttaaa gcgaactgca tatccggata
    86341 cacggatggt cagctgcgga tatttttccg gatgcttaac tgcatcttcc agagtttcac
    86401 gacgcagaac gtttacgttc aggtgttgac caccttcaac gcgaacttct ggtttcactt
    86461 ctactggaac ttcacggtat tcaatgtcac ccagtttgct tactgcaacc acttcatctt
    86521 ctgcataacc tgcttttgca acgatgcaac gtgcttcgcc tttttcgctg tccagcagcc
    86581 agaaagagtt cagcagatcg tcgttagcgg ctttagtaat ctggatacct gtaatcatgt
    86641 gatgcctccc cggcaaaatt atttgatttg ttcagcctgt cgcggccaat tggtaaaacc
    86701 attgttgctt gagtgtatat atactcctca aacccccttg attctttgat ttaaatcaac
    86761 aaaaaccaca catcgattgt ggtcgcaaat ggattttatt gttttacatc aacttatgcg
    86821 ggtgtgaaat tttaccaatt tacatttttt tgcactcgtt taagtctaaa aaatgagcat
    86881 gattttgttc tgtagaaaga agcagttaag ctaggcggat tgaagattcg caggagagcg
    86941 agatggctaa cgaattaacc tggcatgacg tgttggctga agagaagcag caaccctatt
    87001 ttcttaatac ccttcagacc gtcgccagcg agcggcagtc cggcgtcact atctatccac
    87061 cacaaaaaga tgtctttaac gcgttccgct ttaccgagtt gggtgacgtt aaagtggtga
    87121 ttctcggcca ggatccttat cacggaccgg gacaggcgca tgggctggca ttttccgttc
    87181 gccccggaat cgccactcct ccgtcactgc tgaatatgta taaagagctg gaaaatacta
    87241 ttccgggctt cacccgccct aatcatggtt atcttgaaag ctgggcgcgt cagggcgttc
    87301 tgctactcaa tactgtgttg acggtacgcg caggtcaggc gcattcccac gccagcctcg
    87361 gctgggaaac cttcaccgat aaagtgatca gcctgattaa ccagcatcgt gaaggcgtgg
    87421 tgtttttgtt gtggggatcg catgcgcaaa agaaaggggc gattatagat aagcaacgcc
    87481 atcacgtact gaaagcaccg catccgtcac cgctttctgc gcatcgtgga ttctttggct
    87541 gtaaccattt tgtgctggca aatcagtggc tggaacaaca tggcgagacg ccgattgact
    87601 ggatgccagt attaccggca gagagtgagt aaatttgcgg gaaaatgccg gatggcagag
    87661 ttgccacccg gctgatttat caggctttat tctgacgcca ccattcacca agcaaaacgc
    87721 ctgttgcaac agagatattc agcccggcaa cgttgcccgt accgtcaatc ttcacgcgca
    87781 gatcgttcgg atcgcgtgcg gcatccggta acccttcata ttcctgaccc agcaccagta
    87841 ccattttcgc tggcagactg gttttgaaca gcggtttacc ctgctcgctg gaagttgtca
    87901 ctacggtgta acccgcctga cggaaatcat ccagcacgtt aacaatgttg tcgccagtaa
    87961 tcggctgaac gtgctctgcg ccgccttctg cggtacggat agccgcaccc gactctagca
    88021 gtgccgcatc ctgcaccaca acacctttca cgccgaagtg cgcgcagctg cgcatcatgc
    88081 cacccaggtt atgcgggtta gattcgtttt ccagtgccag aacgcaatcc tgcgcacctg
    88141 cctggcttac ccactgctgc acggttgtgc cattacgctt tttgatcagg aagcaaacgc
    88201 cgccgtgatg ttccgtgcct gacgcttttg tcaattccgc ttcatccacc acatggtacg
    88261 ctttgcggtt tgccgccatc cagcgcaagg cttctttaaa acgtggcgtt acgctctgga
    88321 taaaccaggc gcgaacaatc gcttccggac ggctctggaa cagtgcctga catgcatttt
    88381 cgccgtagac gcgggtttct tccgcacgct gacgacgcaa cacttccgga tcaataaaac
    88441 ttttaccact gataccaccg tgatcggcct tttccggcgt ctcatcaccc ggcgcgcggg
    88501 aaaccgtgcg ccacggcgac tcttcccact tgcggtcacg gggctgactc tgtttgtcat
    88561 cgcgggcggg gcgacggcca ccgtcagcac gagattttcc tggacgcccg ccccctttcc
    88621 cggtacgcgg gttgtgggta cgtttatcag aatcatcatc actgcggaca tacatcactt
    88681 tgaccttgcc gcttttacct ttcatttcat cgttcatgct tttctccacc agcgctgcgc
    88741 gaagcgcgca gattacccga agtccgcgcg gttcgccatg atttcgtacc aaagcctgcg
    88801 actatcatac ctattgaata aaacagattg ttgtctggaa caatgtcccc gataatatgt
    88861 aacatattag aaacataccg gcgtcgttgc cgataagtct ccttactcat cccgaggtta
    88921 gttatgaata ccgtttgtac ccattgtcag gccatcaatc gcattcccga cgatcggatc
    88981 gaagatgcgg caaaatgcgg acgctgcggt cacgacttgt ttgacggaga ggtgattaat
    89041 gcgaccggtg aaacgctcga caaattgctg aaagatgatc tacctgtggt gatcgacttc
    89101 tgggcaccgt ggtgcggccc ctgccgtaat ttcgcaccaa tttttgaaga tgtcgcgcaa
    89161 gagcgtagcg gtaaagtgcg ctttgtgaaa gtgaataccg aagctgaacg tgaattgagc
    89221 agtcgctttg gaattcgtag tataccgacg atcatgattt tcaaaaacgg tcaggttgtc
    89281 gacatgctta atggcgcagt accgaaagcg ccgttcgaca gctggttgaa cgaatctctt
    89341 taatcttacc ggggcgcatc ttgtgccccg ttttctcctc tgcgacaatg gcgttttttc
    89401 gacgctctct tatgaccgaa aacgctgttc tccagttacg cgccgaacgt attgcgcgcg
    89461 caacacgtcc ttttcttgcc cgcggtaatc gcgttcgtcg ctgccaacgc tgtcttttgc
    89521 cagagaaatt atgtctctgt tcaacaatca cgccagcgga agcaaaaagt cgcttctgtt
    89581 tgctgatgtt cgacaccgag ccaatgaagc ccagtaatac cgggcgtctg attgctgata
    89641 ttttgcctga taccgttgcg tttcaatggt cgcgtaccga accctcgcag gatttgctgg
    89701 atctggtaca aaacccggat tatcagccaa tggtggtctt tcccgcctcg tatgctgatg
    89761 agcaacggga agtgatcttc acaccacctg ccggtaagcc accgctgttt atcatgctcg
    89821 atggtacctg gccggaagct cgcaagatgt ttcgtaaaag tccgtatctg gataatcttc
    89881 ccgtcatttc cgtcgatctt tcccggcttt ctgcctatcg cctgcgtgaa gcccaggctg
    89941 aaggccaata ttgtactgcc gaggtagcca tcgcactgtt agatatggcc ggcgataccg
    90001 gggcggcagc agggttaggc gagcatttta cccgctttaa aacacgctat ctggcaggaa
    90061 aaacgcaaca tctgggtagc atcacagcag aacagttaga aagcgtttaa aatcattcgg
    90121 tcacttctgc gggagaccgg tatgagtcag cgaggactgg aagcactact gcgaccaaaa
    90181 tcgatagcgg taattggcgc gtcgatgaaa cccaatcgcg caggttacct gatgatgcgt
    90241 aacctgctgg cgggaggctt taacggaccg gtactcccgg tgacgccagc ctggaaagcg
    90301 gtgttgggtg tgttggcctg gccggatatt gccagcttgc cctttacacc cgaccttgcg
    90361 gttttatgta ccaatgccag ccgtaatctt gctcttctgg aagagctcgg cgagaaaggc
    90421 tgtaaaacct gcattattct ttccgccccg gcatcgcaac acgaagatct ccgcgcctgc
    90481 gccctgcgcc ataacatgcg cctgcttgga ccaaacagtc tgggtttact ggctccctgg
    90541 caaggtctga atgccagctt ttcgccggta cctattaaac gcggcaagct ggcgtttatt
    90601 tcacaatcgg ctgccgtctc caacaccatc ctcgactggg cgcaacagcg tgagatgggc
    90661 ttttcctact ttattgcgct cggcgacagc ctggatatcg acgttgatga attgcttgac
    90721 tatctggcac gcgacagtaa aaccagcgcc atcctgctct atctcgaaca gttaagcgac
    90781 gcgcgacgct ttgtttcggc ggcccgtagt gcctcgcgta ataaaccgat tctggtgatt
    90841 aaaagcggac gtagcccggc ggcacagcga ctgctcaaca cgacggcagg aatggacccg
    90901 gcatgggatg cggctattca gcgtgccggt ttgttgcggg tacaggacac ccacgaactg
    90961 ttttcggcgg tagaaaccct tagccatatg cgcccgctgc gtggcgaccg gttgatgatt
    91021 atcagcaacg gtgctgcgcc tgccgcgctg gcgctggatg ccttatggtc acgcaatggc
    91081 aagctggcaa cactaagcga agaaacctgc cagaaactgc gcgatgcact gccaggacat
    91141 gtggcaatct ctaacccgct cgatctgcgc gatgacgcca gcagcgagca ttatgtcaaa
    91201 acgctggaca ttctgctcca tagccaggat tttgatgcgc tgatggttat tcattcgccc
    91261 agcgccgctg ctcccgcaac agaaagtgcg caagcattaa ttgaagcggt aaagcatcat
    91321 ccccgcagca agtatgtttc tctgctgacg aactggtgcg gcgagcactc ctcgcaagag
    91381 gcacgacgtt tattcagcga agccgggctg ccgacctacc gtaccccgga aggaaccatc
    91441 actgctttta tgcatatggt ggagtaccgg cgtaatcaga agcaactacg cgaaacgccg
    91501 gcgttgccca gcaatctgac ttccaatacc gcaaaagcgc atcttctatt gcaacaggcg
    91561 attgccgaag gggctacgtc gctcgatacc catgaagttc agcccatcct gcaagcgtat
    91621 ggcatgaaca cgctccctac ctggattgcc agtgatagca ccgaagcggt gcatattgcc
    91681 gaacagattg gttatccggt ggcgctgaaa ttgcgttcgc cggatattcc acataaatcg
    91741 gaagttcagg gcgtcatgct gtacctgcgt acagccaatg aagtccagca agcggcaaac
    91801 gctattttcg atcgcgtaaa aatgacctgg ccgcaggcgc gggtccacgg cctgttggtg
    91861 caaagtatgg ctaaccgtgc tggcgctcag gagttgcggg ttgtggttga gcacgatccg
    91921 gtttttgggc cgttgatcat gctgggtgaa ggcggtgtgg agtggcgtcc tgaagatcaa
    91981 gccgtcgtcg cactgccgcc gctgaatatg aacctggccc gctatctggt tattcagggg
    92041 atcaaaagta aaaagattcg tgcgcgcagt gcgctacgcc cattggatgt tgcaggcttg
    92101 agccagcttc tggtgcaggt ttccaacttg attgtcgatt gcccggaaat tcagcgtctg
    92161 gatattcatc ctttgctggc ttctggcagt gaatttaccg cgctggatgt cacgctggat
    92221 atcgcgccgt ttgaaggcga taacgagagt cggctggcag tgcgccctta tccgcatcag
    92281 ctggaagaat gggtagaatt gaaaaacggt gaacgctgct tgttccgccc gattttgcca
    92341 gaagatgagc cacaacttca gcaattcatt tcgcgagtca ccaaagaaga tctttattac
    92401 cgctacttta gcgagatcaa cgaatttacc catgaagatt tagccaacat gacgcagatc
    92461 gactacgatc gggaaatggc gtttgtagcg gtacgacgta ttgatcaaac ggaagagatc
    92521 ctcggcgtca cgcgtgcgat ctccgaccct gataacatcg atgccgaatt tgccgtgctg
    92581 gttcgctcgg atctcaaagg gttaggctta ggtcgacgct taatggaaaa gttgattacc
    92641 tatacgcgag atcacggact acaacgtctg aatggtatta cgatgccaaa caatcgtggc
    92701 atggtggcgc tggcccgcaa gctcgggttt aacgttgata tccagctcga agaggggatc
    92761 gttgggctta cgctaaatct tgcccagcgc gaggaatcat gagtaaggta ctggaaatgt
    92821 tgaccactta atcgggactg gtgttattat tgcccgctta tgtcgtctgc attgcacaga
    92881 ggacccttca atgaacagag aagaaatgca ctgtgatgtt gtcaaaattt aagcgtaata
    92941 aacatcaaca acaccttgcc caactaccca agatttctca atcagttgat gatgtcgatt
    93001 tcttttacgc tcccgccgac ttccgggaga cgctgctgga aaaaatagcc agcgcgaagc
    93061 agcgcatttg cattgtcgcc ctgtatctcg aacaggatga cggtggcaaa ggcattctga
    93121 acgcgttgta tgaagctaaa aggcagcgtc cggaactgga tgtgcgggtg ctggtcgact
    93181 ggcatcgtgc acaacgtgga cgcattggcg ctgcggcatc taacactaac gctgactggt
    93241 actgccgcat ggcgcaggaa aatccgggcg tagatgttcc ggtttatggc gttccaatca
    93301 atacccgtga agcccttggc gttctgcact ttaaaggctt tatcatcgac gatagcgtac
    93361 tttatagcgg tgccagcctg aacgatgttt acctgcatca gcacgataaa tatcgctacg
    93421 accgttatca ccttatccgt aaccgtaaga tgtcagacat tatgtttgaa tgggttacac
    93481 agaatattat gaatggccgc ggcgttaatc gtctggatga tgttaatcgg ccaaaaagcc
    93541 cggaaatcaa gaacgatatt cgtctgttcc gccaggagct gcgtgatgcc gcttatcatt
    93601 tccagggcga tgccgacaac gatcagcttt ccgtaacgcc gctagtgggg ctggggaaat
    93661 cgagtctgtt gaacaagacc attttccatc ttatgccttg tgccgagcag aaactaacca
    93721 tctgtacgcc atacttcaac ctgccagcaa tccttgtgcg caatattatc cagttgctgc
    93781 gcgaagggaa aaaggtcgaa attattgttg gtgataaaac cgcgaacgac ttctacattc
    93841 cggaagatga acctttcaag ataattggcg cattgcctta tctctatgag atcaatctgc
    93901 gtcgtttcct gagccgtttg cagtattacg tcaatactga ccagctagtg gttcggttat
    93961 ggaaagatga cgacaacacc tatcacctaa aagggatgtg ggttgatgat aagtggatgt
    94021 tgatcaccgg taataacctg aacccgcgcg cctggcgtct ggatctggaa aacgccattt
    94081 tgatccacga tccacaactg gagcttgcgc cgcagcgaga gaaagaactg gagctgatcc
    94141 gcgagcatac caccatcgtt aagcactatc gcgatctgca aagtattgcc gattatccgg
    94201 tgaaggttcg taaactcatc cgccgtttgc gccgtatccg catcgaccga ttaattagcc
    94261 gcatcctgta atcacaaccc cgtcctgtac ggggtttgtt ttttggaggc cacgttttgc
    94321 gtattctttt tgtctgttcg ctattgttac tttctggatg cagccatatg gctaacgata
    94381 gctggagcgg gcaggataaa gctcaacact ttatcgcctc ggcgatgctt tccgccgccg
    94441 gaaatgaata ttcacagcat caggggatga gccgggatcg cagtgccatg tttggattga
    94501 tgttctctgt cagtttgggg gcgtcaaaag agctttggga tagccgcccc gaagggagcg
    94561 gctggagctg gaaggatttg gcctgggatg tcgccggtgc aagcaccggc tataccgtct
    94621 ggcaactgac ccgtcactaa agacgcatcc ccttcccttt gcgatgtagc atcaaagaaa
    94681 ccagaaacgc caccacggcc atcaaggtca cataccagaa gaaggctgtt tccattccta
    94741 ttgatttcag cgacaacgct acgtactccg ccgaaccacc aaatatagca ttagcgaccg
    94801 catatgacag accaacgcct aatgcgcgaa cctgtgccgg gaacatctca gccttcagta
    94861 ttccactgat tgatgtataa aaactcacta tcagcagggc acacatcacc agaccaaaag
    94921 cggcataagg cgaggaaacg ttttgcaatg ctgagagaat aggaacggta aaaatggctg
    94981 ccagtgaacc gaaacataac attgaggtac ggcgaccaat cttatccgac agcgcgccaa
    95041 tgagtggttg aataagcatg aatacaaaca atgcggcagt cataatgcca ctcgccacgt
    95101 tggcatgcat tcccgcagta tttaccagat acttctgcat ataagtggtg aaggtataga
    95161 aacaaaggga gcccgcagcg gtaaaaccga gaaccatgat gaatgcacgg cgattgcgcc
    95221 ataatccttt cagagatcca gcttctttta aagcgcgcgt ttcttgttgc gaagtttcat
    95281 ctaactgacg acgtaaccac aacgccacaa cagctaacac agctcctaac gcgaaaggaa
    95341 tacgccatcc ccactctctg agtgcagcgt cttccatggt gtgttgtaaa accacgacaa
    95401 ccagtagggc tagcagttgt ccgccgatca acgtcacata ctgaaatgat gcgtaaaaac
    95461 ctttgcgccc ttcaacggca acttcactca tataggtggc gctggtgcca tattctccgc
    95521 caacggataa tccctgaaat aaacgcgcga gaagcaataa tgccggagcc cacgtaccga
    95581 ttgtttcata accaggcaaa caggcgatta ccagtgagcc gaaacacatc atacacaccg
    95641 atagcagcat cgattttttg cgaccatgtt tatcggctat gcggccaaat agccaaccgc
    95701 ctattgggcg catgaggaat cccgcagcaa aaacacctgc tgtttgtagt agttgagtcg
    95761 tcgtgttccc ggaagggaag aagatgtggg caaagtagag tgaacagaac gagtagacat
    95821 agaaatcgaa ccactcgacc agattacctg aagaggcccc cacaatcgcc caaatgcggc
    95881 gacgtgtatc actacttgtc agtttgctgt ctgccgttac agtactttca gccataccat
    95941 tatgtctcct gccgtaatcc gatgcttttg tcggtcgctt ttgtttattt cttttggtaa
    96001 agaaaatgtt atacatttgt tgcgtgtcat tatgcaacct taaccatgaa tttagttagc
    96061 aggaaaatgg ttattgagga gcttaaggat aaatttctgg tgaggagaac acgtatggag
    96121 gtgggcaagt tggggaagcc gtattcgctg ctgaatctgg catatgtggg agtataagac
    96181 gcgcagcgtc gcatcaggca tttttttcac caacgcaaaa aggccatccg tcaggatggc
    96241 ctttcgctt
//
LOCUS       DAZFFG010000006        87995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFFG010000006 DAZFFG010000000
VERSION     DAZFFG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.006"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(55..813)
                     /gene="loiP"
                     /locus_tag="ACHJ7J_000589"
                     /gene_synonym="yggG"
     CDS             complement(55..813)
                     /gene="loiP"
                     /locus_tag="ACHJ7J_000589"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO4998868.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            1091..3082
                     /gene="tkt"
                     /locus_tag="ACHJ7J_000590"
     CDS             1091..3082
                     /gene="tkt"
                     /locus_tag="ACHJ7J_000590"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4998869.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKEAAWNEKFAAYAKAFPQEAAEFTRRMKGEMP
                     SDFDAKANEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            3396..3839
                     /gene="cmtB"
                     /locus_tag="ACHJ7J_000591"
     CDS             3396..3839
                     /gene="cmtB"
                     /locus_tag="ACHJ7J_000591"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO4998870.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSATDADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            3867..5255
                     /gene="cmtA"
                     /locus_tag="ACHJ7J_000592"
     CDS             3867..5255
                     /gene="cmtA"
                     /locus_tag="ACHJ7J_000592"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO4998871.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAKSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            5270..6547
                     /gene="yggP"
                     /locus_tag="ACHJ7J_000593"
     CDS             5270..6547
                     /gene="yggP"
                     /locus_tag="ACHJ7J_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4998872.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLNAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            6544..7509
                     /gene="glpX"
                     /locus_tag="ACHJ7J_000594"
     CDS             6544..7509
                     /gene="glpX"
                     /locus_tag="ACHJ7J_000594"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4998873.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGKGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            7531..8040
                     /gene="fumE"
                     /locus_tag="ACHJ7J_000595"
     CDS             7531..8040
                     /gene="fumE"
                     /locus_tag="ACHJ7J_000595"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO4998874.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     EAPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            8037..8750
                     /gene="yggC"
                     /locus_tag="ACHJ7J_000596"
     CDS             8037..8750
                     /gene="yggC"
                     /locus_tag="ACHJ7J_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO4998875.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIKRVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPIEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLMNS
                     RQANLIVEMTEEGRYHFTS"
     gene            9035..10054
                     /gene="epd"
                     /locus_tag="ACHJ7J_000597"
     CDS             9035..10054
                     /gene="epd"
                     /locus_tag="ACHJ7J_000597"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO4998876.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            10104..11267
                     /gene="pgk"
                     /locus_tag="ACHJ7J_000598"
     CDS             10104..11267
                     /gene="pgk"
                     /locus_tag="ACHJ7J_000598"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO4998877.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11482..12561
                     /gene="fbaA"
                     /locus_tag="ACHJ7J_000599"
     CDS             11482..12561
                     /gene="fbaA"
                     /locus_tag="ACHJ7J_000599"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO4998878.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12625..12723)
                     /locus_tag="ACHJ7J_000600"
                     /pseudo
     CDS             complement(12625..12723)
                     /locus_tag="ACHJ7J_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12919..13794
                     /gene="mscS"
                     /locus_tag="ACHJ7J_000601"
     CDS             12919..13794
                     /gene="mscS"
                     /locus_tag="ACHJ7J_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO4998879.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKENKENEAVTR"
     gene            complement(13836..14879)
                     /locus_tag="ACHJ7J_000602"
     CDS             complement(13836..14879)
                     /locus_tag="ACHJ7J_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO4998880.1"
                     /translation="MTFKHFLLSLVSGLLLISSHNVAAEKILRYTDHEPYGGMRTQII
                     KEIFFAEIEKESQGRLKIEPHWNGETAISYDALTTISDGSKADMGIVVPEYTAKQLPL
                     HQIFKSFAIGPDHGASQVEFFRRVYAEIPEFNAELERNNIVNLQFFLGYPVGFFSTRP
                     IDKLTALQGTTWRTASFWHRAYLTHTGAKTVTLPWNEQITTALRDGQLDGLMVNLDSG
                     YDIHAERAAPNVLLSPSLWLGHVYLLVMNKQTWENLDNRDREAIQRAAITTEKALGKA
                     LDNNLISMVKTLEQEGAQVRYLKKSELDAWQKAIGYQQEQTQWVEKQNNEGVEKAGGV
                     MQKVANILDETMR"
     gene            complement(14934..15863)
                     /locus_tag="ACHJ7J_000603"
     CDS             complement(14934..15863)
                     /locus_tag="ACHJ7J_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998881.1"
                     /translation="MHAYDLTLLPHPIRENMPRQQGWCFGLPPGITPEQWPLDPNNGF
                     PLNHGFTLLLPEDYRIYGPEIVALSFFAVAPEHNDGGTPCIEELLDVFENFESGIPPE
                     DPDLYVFWLAEKQRHPRLFRMEDILGCSYAVILLTQQEFNGPFCEPPELIPNHYRNQQ
                     DTPEWMTTGSAFSYFQASVRPKDTPESNFVYRKMGTIPEQSLAFNLAISCKPRAFDPN
                     AGISPTERNNTEYQSIRYFSKDAEGKSKCETHQWHSAHQPDHLGGSMVPLQSIPDGMS
                     PFYIEFEEYFGGYNFGTGNAWLDFKNMKFDFSC"
     gene            16067..16702
                     /gene="argO"
                     /locus_tag="ACHJ7J_000604"
     CDS             16067..16702
                     /gene="argO"
                     /locus_tag="ACHJ7J_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO4998882.1"
                     /translation="MISYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            16795..17535
                     /gene="yggE"
                     /locus_tag="ACHJ7J_000605"
     CDS             16795..17535
                     /gene="yggE"
                     /locus_tag="ACHJ7J_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO4998883.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            17703..18599
                     /gene="ygfI"
                     /locus_tag="ACHJ7J_000606"
     CDS             17703..18599
                     /gene="ygfI"
                     /locus_tag="ACHJ7J_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4998884.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(18596..20074)
                     /gene="scpC"
                     /locus_tag="ACHJ7J_000607"
     CDS             complement(18596..20074)
                     /gene="scpC"
                     /locus_tag="ACHJ7J_000607"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO4998885.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(20098..20883)
                     /gene="scpB"
                     /locus_tag="ACHJ7J_000608"
     CDS             complement(20098..20883)
                     /gene="scpB"
                     /locus_tag="ACHJ7J_000608"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO4998886.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGAKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(20894..21889)
                     /gene="meaB"
                     /locus_tag="ACHJ7J_000609"
     CDS             complement(20894..21889)
                     /gene="meaB"
                     /locus_tag="ACHJ7J_000609"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO4998887.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIRDGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(21882..24026)
                     /gene="scpA"
                     /locus_tag="ACHJ7J_000610"
     CDS             complement(21882..24026)
                     /gene="scpA"
                     /locus_tag="ACHJ7J_000610"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO4998888.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(24230..25123)
                     /gene="argP"
                     /locus_tag="ACHJ7J_000611"
     CDS             complement(24230..25123)
                     /gene="argP"
                     /locus_tag="ACHJ7J_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO4998889.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            complement(25194..25553)
                     /locus_tag="ACHJ7J_000612"
     CDS             complement(25194..25553)
                     /locus_tag="ACHJ7J_000612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase"
                     /protein_id="HFO4998890.1"
                     /translation="MDSWRHRHYPFIAQLEYLNQHGASRVPVDLWYCVSRSEDAWYVD
                     KLTSLCAQASVTLHLLDAHKGERLQSHYLTDKIANKGDTHFWFCGPQSFAKALSKGLY
                     ENGIASQNFHYDRFCMR"
     gene            complement(25499..26518)
                     /locus_tag="ACHJ7J_000613"
     CDS             complement(25499..26518)
                     /locus_tag="ACHJ7J_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HFO4998891.1"
                     /translation="MKKANLTGTLVCFVACIIFLSSSDIFREKPIALIWFIRQNTLFF
                     TGTLAWCLMTLTMCLSLRSSWLNKVLGGLDKAWRLHKWAGICAIAFAFAHWLDEKLPQ
                     LFVAFGWLTHPGKIVDINLTPVQENWLHAGLLAGECAMFIMIAMVFVSLSKKVPYHLF
                     HLVHRLFPVFYLAIAFHVFTALFKSYWWETPAAYLLILITIPGVFAAFISLLKLNGNK
                     NKHQATIKSIVNHPGQITEVILELEHVIDYSPGQFAFLTFAHSKESHPFTIASYAIEK
                     NTLRFAIKNLGDYTSTLASSITIGQSALVEGPWGKFDFTLPCSRQVWIAGGIGITPLS
                     PSLSI"
     gene            complement(26580..26840)
                     /locus_tag="ACHJ7J_000614"
     CDS             complement(26580..26840)
                     /locus_tag="ACHJ7J_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFO4998892.1"
                     /translation="MSIKQNLFAALFLCTLTGHAFAATEIHKPDNNLEQLSKVSMSTH
                     NSNPEGVESQIKEKAEELQASNYYISSFSGKNYIHATVIFYK"
     gene            complement(26857..27486)
                     /locus_tag="ACHJ7J_000615"
     CDS             complement(26857..27486)
                     /locus_tag="ACHJ7J_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFO4998893.1"
                     /translation="MKILHIDSSPMLKHSSSRKLSQTLVEALCQHFPKATIIHRDVGL
                     TPPPHLREETFMALCDKAECGSAQIRQEVNGIHAAIEELASCDVLIIGAPMINHSVSS
                     GLKTWIDQVCQAGLTFRFTSAGAVGLLTDKPTFIVSTRGGIYCDEAHLALDHQESYLQ
                     ATLKLMGITNTHILRAEGVDKTQPGRELAEQNALAQIPELITQVFNHYL"
     gene            27587..28483
                     /locus_tag="ACHJ7J_000616"
     CDS             27587..28483
                     /locus_tag="ACHJ7J_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4998894.1"
                     /translation="MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERL
                     GVRLIQRTTRRLALTAAGEQFAVECRALVAQGERARAVVMAAQESPQGMLRVSAPVFM
                     AETWFGEFVSEFIARWPLAKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKL
                     HTQADILVASSQWLARHPQVKAIDDLNRVDGILRLTNNGGSQWQLINGEQEITLQPEA
                     RLQTNNIRVMLQAVLGGNGVALLPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGR
                     KGMPAIARYFMDELTTRLANGV"
     gene            28539..29198
                     /gene="rpiA"
                     /locus_tag="ACHJ7J_000617"
     CDS             28539..29198
                     /gene="rpiA"
                     /locus_tag="ACHJ7J_000617"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO4998895.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29454..30686
                     /gene="serA"
                     /locus_tag="ACHJ7J_000618"
     CDS             29454..30686
                     /gene="serA"
                     /locus_tag="ACHJ7J_000618"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO4998896.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            30925..30984
                     /locus_tag="ACHJ7J_000619"
                     /pseudo
     CDS             30925..30984
                     /locus_tag="ACHJ7J_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
     gene            complement(31085..31687)
                     /gene="fau"
                     /locus_tag="ACHJ7J_000620"
     CDS             complement(31085..31687)
                     /gene="fau"
                     /locus_tag="ACHJ7J_000620"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO4998897.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKIWEW"
     gene            complement(31708..31891)
                     /gene="ssrS"
                     /locus_tag="ACHJ7J_000621"
     ncRNA           complement(31708..31891)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHJ7J_000621"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(31933..32262)
                     /gene="zapA"
                     /locus_tag="ACHJ7J_000622"
     CDS             complement(31933..32262)
                     /gene="zapA"
                     /locus_tag="ACHJ7J_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4998898.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            32430..33008
                     /gene="ygfB"
                     /locus_tag="ACHJ7J_000623"
     CDS             32430..33008
                     /gene="ygfB"
                     /locus_tag="ACHJ7J_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO4998899.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            33034..34356
                     /gene="pepP"
                     /locus_tag="ACHJ7J_000624"
     CDS             33034..34356
                     /gene="pepP"
                     /locus_tag="ACHJ7J_000624"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO4998900.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARK"
     gene            34356..35534
                     /gene="ubiH"
                     /locus_tag="ACHJ7J_000625"
                     /gene_synonym="visB"
     CDS             34356..35534
                     /gene="ubiH"
                     /locus_tag="ACHJ7J_000625"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO4998901.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLECGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLRRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            35557..36759
                     /gene="ubiI"
                     /locus_tag="ACHJ7J_000626"
                     /gene_synonym="visC"
     CDS             35557..36759
                     /gene="ubiI"
                     /locus_tag="ACHJ7J_000626"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO4998902.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PSQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQASDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(36833..36916)
                     /locus_tag="ACHJ7J_000627"
     CDS             complement(36833..36916)
                     /locus_tag="ACHJ7J_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998903.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            37207..38301
                     /gene="gcvT"
                     /locus_tag="ACHJ7J_000628"
     CDS             37207..38301
                     /gene="gcvT"
                     /locus_tag="ACHJ7J_000628"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO4998904.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            38325..38714
                     /gene="gcvH"
                     /locus_tag="ACHJ7J_000629"
     CDS             38325..38714
                     /gene="gcvH"
                     /locus_tag="ACHJ7J_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO4998905.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDVLSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            38832..41705
                     /gene="gcvP"
                     /locus_tag="ACHJ7J_000630"
     CDS             38832..41705
                     /gene="gcvP"
                     /locus_tag="ACHJ7J_000630"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO4998906.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLSDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(41869..43302)
                     /gene="bglA"
                     /locus_tag="ACHJ7J_000631"
     CDS             complement(41869..43302)
                     /gene="bglA"
                     /locus_tag="ACHJ7J_000631"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO4998907.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDILREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGKNL"
     gene            43347..43658
                     /gene="yqfB"
                     /locus_tag="ACHJ7J_000632"
     CDS             43347..43658
                     /gene="yqfB"
                     /locus_tag="ACHJ7J_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO4998908.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDSYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            43822..44481
                     /gene="yqfA"
                     /locus_tag="ACHJ7J_000633"
     CDS             43822..44481
                     /gene="yqfA"
                     /locus_tag="ACHJ7J_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO4998909.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(44558..45538)
                     /gene="ygfZ"
                     /locus_tag="ACHJ7J_000634"
     CDS             complement(44558..45538)
                     /gene="ygfZ"
                     /locus_tag="ACHJ7J_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO4998910.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIQAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            45781..46047
                     /gene="sdhE"
                     /locus_tag="ACHJ7J_000635"
     CDS             45781..46047
                     /gene="sdhE"
                     /locus_tag="ACHJ7J_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO4998911.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            46028..46435
                     /gene="ygfX"
                     /locus_tag="ACHJ7J_000636"
     CDS             46028..46435
                     /gene="ygfX"
                     /locus_tag="ACHJ7J_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO4998912.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(46475..46996)
                     /gene="fldB"
                     /locus_tag="ACHJ7J_000637"
     CDS             complement(46475..46996)
                     /gene="fldB"
                     /locus_tag="ACHJ7J_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO4998913.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            47108..48004
                     /gene="xerD"
                     /locus_tag="ACHJ7J_000638"
     CDS             47108..48004
                     /gene="xerD"
                     /locus_tag="ACHJ7J_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO4998914.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            48029..48739
                     /gene="dsbC"
                     /locus_tag="ACHJ7J_000639"
     CDS             48029..48739
                     /gene="dsbC"
                     /locus_tag="ACHJ7J_000639"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO4998915.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            48745..50478
                     /gene="recJ"
                     /locus_tag="ACHJ7J_000640"
     CDS             48745..50478
                     /gene="recJ"
                     /locus_tag="ACHJ7J_000640"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO4998916.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            50569..51667
                     /gene="prfB"
                     /locus_tag="ACHJ7J_000641"
     CDS             join(50569..50643,50645..51667)
                     /gene="prfB"
                     /locus_tag="ACHJ7J_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO4998917.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            51677..53194
                     /gene="lysS"
                     /locus_tag="ACHJ7J_000642"
     CDS             51677..53194
                     /gene="lysS"
                     /locus_tag="ACHJ7J_000642"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4998918.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(53237..53785)
                     /gene="idi"
                     /locus_tag="ACHJ7J_000643"
     CDS             complement(53237..53785)
                     /gene="idi"
                     /locus_tag="ACHJ7J_000643"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO4998919.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            53840..53911
                     /locus_tag="ACHJ7J_000644"
     CDS             53840..53911
                     /locus_tag="ACHJ7J_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998920.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(53908..54033)
                     /gene="yqfG"
                     /locus_tag="ACHJ7J_000645"
     CDS             complement(53908..54033)
                     /gene="yqfG"
                     /locus_tag="ACHJ7J_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO4998921.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(54035..55483)
                     /gene="uacT"
                     /locus_tag="ACHJ7J_000646"
     CDS             complement(54035..55483)
                     /gene="uacT"
                     /locus_tag="ACHJ7J_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO4998922.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            55919..57838
                     /gene="ygfT"
                     /locus_tag="ACHJ7J_000647"
     CDS             55919..57838
                     /gene="ygfT"
                     /locus_tag="ACHJ7J_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO4998923.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKAARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKAFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            57838..58326
                     /gene="ygfS"
                     /locus_tag="ACHJ7J_000648"
     CDS             57838..58326
                     /gene="ygfS"
                     /locus_tag="ACHJ7J_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4998924.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(58362..59729)
                     /gene="ghxQ"
                     /locus_tag="ACHJ7J_000649"
                     /gene_synonym="ygfQ"
     CDS             complement(58362..59729)
                     /gene="ghxQ"
                     /locus_tag="ACHJ7J_000649"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO4998925.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(59765..61081)
                     /gene="guaD"
                     /locus_tag="ACHJ7J_000650"
     CDS             complement(59765..61081)
                     /gene="guaD"
                     /locus_tag="ACHJ7J_000650"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO4998926.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(61099..62499)
                     /gene="xanQ"
                     /locus_tag="ACHJ7J_000651"
     CDS             complement(61099..62499)
                     /gene="xanQ"
                     /locus_tag="ACHJ7J_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO4998927.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(62664..65534)
                     /gene="xdhD"
                     /locus_tag="ACHJ7J_000652"
     CDS             complement(62664..65534)
                     /gene="xdhD"
                     /locus_tag="ACHJ7J_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO4998928.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDSLSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFHAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(65531..66310)
                     /gene="ygfM"
                     /locus_tag="ACHJ7J_000653"
     CDS             complement(65531..66310)
                     /gene="ygfM"
                     /locus_tag="ACHJ7J_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO4998929.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(66361..67689)
                     /gene="ssnA"
                     /locus_tag="ACHJ7J_000654"
     CDS             complement(66361..67689)
                     /gene="ssnA"
                     /locus_tag="ACHJ7J_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO4998930.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(67692..70787)
                     /gene="ygfK"
                     /locus_tag="ACHJ7J_000655"
     CDS             complement(67692..70787)
                     /gene="ygfK"
                     /locus_tag="ACHJ7J_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO4998931.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAIFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRPVETNETVTLLVDSLITAIGEQQDTEALNAMGV
                     PLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQ
                     NDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPNR
                     ANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSSN
                     PGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRVE
                     V"
     gene            complement(71109..71687)
                     /gene="mocA"
                     /locus_tag="ACHJ7J_000656"
     CDS             complement(71109..71687)
                     /gene="mocA"
                     /locus_tag="ACHJ7J_000656"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO4998932.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAFQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            71790..72560
                     /gene="yqeC"
                     /locus_tag="ACHJ7J_000657"
     CDS             71790..72560
                     /gene="yqeC"
                     /locus_tag="ACHJ7J_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO4998933.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQT
                     RGRRVLITTTTHMFMPKSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            72608..74233
                     /gene="yqeB"
                     /locus_tag="ACHJ7J_000658"
     CDS             72608..74233
                     /gene="yqeB"
                     /locus_tag="ACHJ7J_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO4998934.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(74454..75386)
                     /gene="arcC"
                     /locus_tag="ACHJ7J_000659"
     CDS             complement(74454..75386)
                     /gene="arcC"
                     /locus_tag="ACHJ7J_000659"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4998935.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLCDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(75434..76819)
                     /gene="hyuA"
                     /locus_tag="ACHJ7J_000660"
     CDS             complement(75434..76819)
                     /gene="hyuA"
                     /locus_tag="ACHJ7J_000660"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO4998936.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLIAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(76872..78083)
                     /gene="ygeY"
                     /locus_tag="ACHJ7J_000661"
     CDS             complement(76872..78083)
                     /gene="ygeY"
                     /locus_tag="ACHJ7J_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO4998937.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(78141..79337)
                     /gene="dpaL"
                     /locus_tag="ACHJ7J_000662"
     CDS             complement(78141..79337)
                     /gene="dpaL"
                     /locus_tag="ACHJ7J_000662"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO4998938.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(79395..80582)
                     /gene="ygeW"
                     /locus_tag="ACHJ7J_000663"
     CDS             complement(79395..80582)
                     /gene="ygeW"
                     /locus_tag="ACHJ7J_000663"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO4998939.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            81061..82839
                     /gene="ygeV"
                     /locus_tag="ACHJ7J_000664"
     CDS             81061..82839
                     /gene="ygeV"
                     /locus_tag="ACHJ7J_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO4998940.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGIVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(82879..83358)
                     /gene="xdhC"
                     /locus_tag="ACHJ7J_000665"
     CDS             complement(82879..83358)
                     /gene="xdhC"
                     /locus_tag="ACHJ7J_000665"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO4998941.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(83355..84233)
                     /gene="xdhB"
                     /locus_tag="ACHJ7J_000666"
     CDS             complement(83355..84233)
                     /gene="xdhB"
                     /locus_tag="ACHJ7J_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO4998942.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRSVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEHTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(84244..86541)
                     /gene="xdhA"
                     /locus_tag="ACHJ7J_000667"
     CDS             complement(84244..86541)
                     /gene="xdhA"
                     /locus_tag="ACHJ7J_000667"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO4998943.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSEMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            86957..87712
                     /gene="actS"
                     /locus_tag="ACHJ7J_000668"
     CDS             86957..87712
                     /gene="actS"
                     /locus_tag="ACHJ7J_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO4998944.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            87791..87864
                     /locus_tag="ACHJ7J_000669"
     tRNA            87791..87864
                     /locus_tag="ACHJ7J_000669"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87823..87825,aa:Gly,seq:ccc)
BASE COUNT        21381 a        21795 c        23083 g        21736 t
ORIGIN      
        1 atgtcagatg cggcgtgaac gccttatctg acctacgttc gacaccacca ggctttactt
       61 aatcccatcc gcgctcatgc gatcgcgaat atgctgggcg cgttcggcgg atgcaggatg
      121 gtcgtcaaac attgagcttt ggcgaccttc ttccagtttt gccagttttt caaagctggt
      181 ggcaagacct gccgggctga tgccgcgttg gcgcagaaga tcgtaagaat aatcatcggc
      241 ttctgcttcc tggcgctggg agaattgcga attaactaat ttctcgccca gatcaccgag
      301 ttgtgattga gataaactgc cgacaatccc gcccgcagag gccgcagcta ctcgcacggc
      361 atttgtacca agtgccacct gcatcccttt cttcacatgg cctaacgcca cgtgccccat
      421 ttcgtgaccg atcaccgctt cgacttcgtt atccgtcatc atatccatca gcccgctata
      481 gacacggata cagccgttag ccattgcaaa ggcgttcaca tccttcgcca tatacacttt
      541 gtaatttacc ggctgaccgt tgatattgtt gcctagcgca ttggcaatag ttgtcaggcg
      601 tttagcgtat tcgctattgg ctggcgcaat cgtcgccttg ctgtccatct cctgacatgc
      661 ctgatcgctc agggttttca cctgcgcatc actcaaactg taagcctgaa aagcttccgc
      721 tcctgatgag agcagtccgt tggagtccat attctggcaa ccagtcagta ccgttgccac
      781 gctcattgct accaataagg cgcgaatttt catgtttttt cttccgcaca aggtcaggat
      841 tattctataa atgcgccgtt tgcaggtgaa ccgacgctca gtctcagtat aaggaaaagc
      901 gcagcggacg ggcgagtaga ttgcgcaaca tgcgagcatg atccagagat ttctgaagcg
      961 gcaaaaggat gttccatgta catgacgcgc ggcttgcggt aaattgttgg caaattttcc
     1021 ggcgtagccc aaaacgcgct gtcgtcaagt cgttaagggc gtgcccttca tcatccgatc
     1081 tggagtcaaa atgtcctcac gtaaagagct tgccaatgct attcgtgcgc tgagcatgga
     1141 cgcagtacag aaagccaaat ccggtcaccc gggtgcccct atgggtatgg ctgacattgc
     1201 cgaagtcctg tggcgtgatt tcctgaaaca caacccgcag aatccgtcct gggctgaccg
     1261 tgaccgcttc gtgctgtcca acggccacgg ctccatgctg atctacagcc tgctgcacct
     1321 caccggttac gatctgccga tggaagaact gaaaaacttc cgtcagctgc actctaaaac
     1381 tccgggccac ccggaagtgg gttacaccgc tggtgtggaa accaccaccg gtccgctggg
     1441 gcagggtatt gccaacgcag taggtatggc gattgcagaa aaaacgctgg cggcgcagtt
     1501 taaccgtccg ggccacgaca ttgtcgacca ctacacctac gccttcatgg gcgacggctg
     1561 catgatggaa ggcatctccc acgaagtttg ctctctggcg ggtacgctga agctgggtaa
     1621 actgattgcg ttctacgatg acaacggtat ttctatcgat ggtcacgttg aaggctggtt
     1681 caccgacgac accgcaatgc gtttcgaagc ttacggctgg cacgttattc gcgacatcga
     1741 cggtcatgac gcagcatcca tcaaacgcgc agtagaagaa gcgcgcgcag tgactgacaa
     1801 accgtccctg ctgatgtgca aaaccatcat cggtttcggt tccccgaaca aagccggtac
     1861 tcacgactcc cacggtgcgc cgctgggtga tgcagaaatc gctctgaccc gcgaacagct
     1921 gggctggaaa tacgcgccgt tcgaaatccc gtctgaaatc tatgcgcagt gggatgcgaa
     1981 agaagcaggc caggcgaaag aagctgcctg gaatgagaaa ttcgcggctt acgccaaagc
     2041 cttcccgcag gaagcggctg aattcacccg tcgtatgaaa ggtgaaatgc cgtctgactt
     2101 cgacgcgaaa gcgaatgaat tcattgcgaa gctgcaggct aacccggcga aaatcgccag
     2161 ccgtaaagcg tcgcagaatg ctatcgaagc gttcggcccg ctgttgccgg aattcctcgg
     2221 cggctccgct gacctggcac cgtctaacct gaccctgtgg tctggttcta aagcaatcaa
     2281 cgaagatgct gcgggtaact acatccacta cggtgttcgc gagttcggta tgaccgcgat
     2341 tgctaacggt atctccctgc acggtggctt cctgccgtac acctccacct tcctgatgtt
     2401 tgtcgaatac gcacgtaacg ccgtacgtat ggctgcgctg atgaaacagc gtcaggtgat
     2461 ggtttacacc cacgactcca tcggtctggg cgaagacggc ccgactcacc agccggttga
     2521 gcaggtcgct tctctgcgcg tgaccccgaa catgtctaca tggcgtccgt gtgaccaggt
     2581 tgaatccgcg gtcgcgtgga aatacggtgt tgagcgtcag gacggtccga ccgcgctgat
     2641 cctctcccgt cagaacctgg cgcagcagga acgtactgaa gagcaactgg caaacatcgc
     2701 gcgcggtggt tatgtgctga aagactgtgc tggtcagccg gaactgatct tcatcgccac
     2761 cggttctgaa gttgaactgg cggttgctgc ctacgaaaaa ctgactgccg aaggcgtgaa
     2821 agcgcgcgtg gtttccatgc cgtctaccga cgcattcgac aagcaggacg cggcttaccg
     2881 tgagtctgta ctgcctaaag cggttactgc acgcgttgcg gtggaagcgg gtattgctga
     2941 ctactggtac aagtatgttg gcctgaatgg cgctatcatc ggtatgacca ccttcggtga
     3001 gtcagctccg gcagagttgc tgttcgaaga gttcggcttc accgtcgaca acgtcgttgc
     3061 gaaagcaaaa gaactgctgt aattagcatt tcgggtaaaa aggtcgcttc ggcgaccttt
     3121 tttattacct tgatatgtcc gtttgcggac aagcaataga taaagcgtgt tgtagatcac
     3181 aaatatttat atgcaataaa tatcaattat gtaatatgca tcacgatatg cgtgttgaca
     3241 tttgttgtta tatctataac tcaatgttat ataagaaatt aataattcac tgttttcaaa
     3301 acaccggttt ccctgctcaa ttgctttcat taaaccgcac gagtatttta tttttaccgc
     3361 cggacggcac cctaaaaata aaataaggat aatttatgcg gcttagtgat tattttccag
     3421 aatcatcaat ctcagttata cattcagcaa aagattggca ggaagctatc gatttctcga
     3481 tggtatcatt gctggataaa aactatatca gcgagaatta cattcaggca attaaagatt
     3541 ccaccattaa caatggccct tattatattc tcgcaccagg cgtggcaatg cctcatgcgc
     3601 gaccggaatg tggggcgctt aaaaccggga tgtctttgac attacttgaa caaggtgttt
     3661 attttccggg gaatgacgaa ccaattaaat tactcatcgg actctctgct accgatgccg
     3721 attcgcacat tggcgctatt caggcgttaa gtgaattact gtgcgaagaa gaaatactcg
     3781 aacaactctt aacagcatca tcagaaaaac aattagcgga cattatcagc cgcggataat
     3841 tactctctcc tctacaggat aatattatgg aaaacaagtc tgctcgtgca aaggtccagg
     3901 cttttggggg ctttttgact gcaatggtca tccccaatat tggtgctttt attgcctggg
     3961 gttttattac tgcgttattt attcccaccg gttggctgcc taacgaacat ttcgccaaaa
     4021 ttgtcggccc gatgattacc tatttattgc ccgtgatgat tggttctacc ggtggtcatc
     4081 tggtcggcgg taaacgcggc gcggtcatgg gcggaatagg tactattggt gtgatcgtcg
     4141 gcgcagagat cccgatgttc cttggctcaa tgattatggg gccgctcggt ggtctggtca
     4201 taaaatatgt tgataaggca ctggaaaaac gcatacctgc cggttttgag atggttatca
     4261 ataacttctc attaggtatc gcggggatgc tcctttgcct gctgggtttt gaagtgatcg
     4321 gcccggcggt gttaattgcc aatactttcg tcaaagagtg tattgaggcg ctggtacatg
     4381 cgggttatct gcctctgctg tcagtcatca atgaaccggc gaaagtgctt ttccttaata
     4441 atgcgatcga tcagggcgtc tattatccgc tgggaatgca acaggcttcg gttaacggta
     4501 aatccatctt ctttatggtg gcctctaacc caggtccggg cctggggctg ctgctggcgt
     4561 ttaccttgtt tggtaaaggg atgagtaaac gttctgcgcc cggggcgatg attattcact
     4621 tcctcggtgg gatccacgaa ctgtatttcc catatgtgct gatgaagccg ctgactatta
     4681 ttgccatgat tgcgggcggt atgtctggca cctggatgtt taacttactg gacggtggtc
     4741 tggtggctgg cccaagtccg gggtctatct ttgcttacct ggcgctgacg ccgaaaggtt
     4801 cgttcctggc gacaattgcc ggtgttacgg taggtaccct ggtgtccttt gctatcactt
     4861 cgctgatact gaagatggaa aaaacggtgg aaacggagag cgaagatgag tttgctaagt
     4921 cagccaatgc ggttaaggcg atgaaacaag agggtgcatt ctcgttaagc agggttaagc
     4981 gtatcgcctt tgtttgcgat gcggggatgg gctccagtgc gatgggcgcg accaccttcc
     5041 gtaaacgcct ggaaaaagcg gggctggcaa ttgaagtaaa acattacgcc atagaaaacg
     5101 tgcctgcgga tgcggatatc gtcgttactc atgccagtct ggaagggcgc gtgaaacgtg
     5161 tgacggataa accactgata ttgattaata actatattgg cgatccaaaa ctcgacactt
     5221 tatttaatca attaaccgcc gaacataaac actgattaca gaggtaaaaa tgaaaaccaa
     5281 agttgctgct atttatggca agcgggatgt ccgtctgcgc gaatttgaac tgccagaaat
     5341 taccgataat gaattactgg tgagtgtgat ttctgacagc gtctgtttat ccacctggaa
     5401 agcggcgtta ctcggtagtg aacataaacg cgtacccgac gatttagaaa atcatccggt
     5461 cattaccggg catgaatgtg ccggggttat tgtcgaagtg ggtaaaaatc tcactggcaa
     5521 atataaaaaa ggccagcgtt ttgtattgca accggcgatg gggttaccaa gcggatattc
     5581 agcgggctac agctacgaat attttggcgg caatgccact tatatgatta ttcccgaaat
     5641 cgccattaat ttgggctgcg tattaccgta tcacggctct tattttgctg cggcgtcgct
     5701 ggcagagcct atgtgctgca ttattggtgc ttatcatgcc aattatcaca ccacgcaata
     5761 tgtttatgag catcgcatgg gcgtcaaacc tggcggcaat attgcactgc tggcgtgtgc
     5821 aggtccgatg ggcattggcg ctatcgatta cgccattaac ggcggcatac aaccgtcgcg
     5881 ggtggtggtg gtcgatatcg acgacaaacg tctggcgcag gtacagaagc tgctgccggt
     5941 ggaactggcg gccagtaaag gcattgagct ggtgtatgtg aataccaaag ggatgagcga
     6001 tcctgtccag atgctgcggg cgctgacagg agatgccggg ttcgatgaca tttttgttta
     6061 tgcggcggtg cctgctgtcg ttgagatggc tgatgaatta ctggcggaag atggctgtct
     6121 gaacttcttt gccgggccga cggataaaaa cttcaaagtg ccgtttaatt tctacaacgt
     6181 tcattacaac agcacgcacg tcgtcggtac atccggcggt tcaacggacg acatgaaaga
     6241 ggcgattgcc cttagcgcca ctgggcagtt acagccgtcg tttatggtga cccatatcgg
     6301 tggcctgaat gcggtgccag aaaccgtgct caatctgccg gatatccctg gcggtaaaaa
     6361 actcatttat aacggcgtga ccatgccgct cactgccatt gccgattttg ccgaaaaagg
     6421 caaaaccgat ccgctgttta aagagttggc gcggctggtt gaggaaacgc acggcatctg
     6481 gaatgaacag gccgagaaat atctgctggc acaatttggc gttgatatcg gggaggccgc
     6541 gcaatgatgt ccctggcgtg gccattattt cgcgttacgg aacaggcagc gttggctgcc
     6601 tggccgcaaa ccggatgtgg cgacaaaaat aaaattgatg gcctggcggt caccgcgatg
     6661 cgccaggcat taaacgacgt cgcttttcgt ggacgagtag ttatcggcaa aggagaaatt
     6721 gaccatgcgc cgatgctgtg gattggcgaa gaggtgggca aaggcgatgg gccagaagtc
     6781 gatatcgcgg ttgatcccat tgaaggcacg cggatggtgg cgatggggca gagcaatgcg
     6841 ctggcggtca tggctttcgc ccctcgagat agcctgttac atgcgcccga tatgtatatg
     6901 aaaaagctgg ttgttaatcg actggctgcc ggggcgattg atctgtcttt gcctctggcg
     6961 gacaacttgc gtaacgtggc gcgggcgtta ggtaaaccgc tggacaagct gcgcatggtt
     7021 acgctggata aaccgcgtct gagtgccgcg attgaagaag caacacagct gggcgtgaag
     7081 gtttttgccc tgccggatgg cgatgtcgcc gccagcgtgc tgacctgctg gcaggataat
     7141 ccttacgatg tgatgtacac catcggcggt gcgccggaag gcgtgatttc tgcctgtgcc
     7201 gttaaagcat taggcgggga tatgcaggcc gagctgattg acttttgtca ggctaaaggg
     7261 gattacacgg aaaatcggca gattgcagag caggagcgca agcgttgcaa ggcaatgggc
     7321 gttgacgtta accgtgtgta ctcgctcgat gaactggtga gggggaacga tatcctcttt
     7381 agcgctacgg gcgtgacggg cggtgagctg gtgaacggta tccaacagac ggcgaatggg
     7441 gtgcggacgc agacattact gatcggcggc gcggaccaaa cgtgtaatat aatagactcc
     7501 ctgcattgat ggtgatctat cggaagacca atggcgacgc tgacagaaga tgatgtgctt
     7561 gagcaactgg atgcacagga caatttattt tcatttatga aaactgcgca ttctattttg
     7621 ctccagggga tacgccagtt tctgccgtcg ctgtttgtcg ataacgatga agagatcgtg
     7681 gaatatgcag tgaagccgtt actcgcccaa agcggcccgc ttgacgatat tgatgttgcg
     7741 ctgcgtttga tttatgcgct ggggaaaatg gataaatggc tgtacgccga tatcactcat
     7801 ttttcccagt tctggcatta cctgaacgaa caggatgaag cgcccggatt tgccgatgac
     7861 atgacctggg attttatcag caacgtcaat agcataaccc gcaatgcgat gctctatgac
     7921 gcgttaaaag cgatgaagtt cgctgatttc gccgtctggt cagaggcgcg ttttagcggg
     7981 atggtcaaaa cggcgctgac gctggcagta acgaccacct taaaggaatt aacgccgtga
     8041 aaattgaatt aacggtgaat gggctgaacg tgcaagcgca gtaccatgat gaagagataa
     8101 aacgcgtgca taaaccgttg ctgcgtatgc tggcagcctt acagacggta aatccgcagc
     8161 ggcgtactgt ggtttttctt tgcgctccgc cggggacggg gaaatctacc ctaaccacct
     8221 tctgggaata tctcgcgcaa caagacccgg aactgcccgc tattcagacg ctgccgatgg
     8281 atggttttca ccattacaat agctggctgg atgcgcatca attgcgcccc ttcaaaggcg
     8341 caccagagac atttgacgta gcgaaactgg cggaaaatct gcgccaggtc gtggaagggg
     8401 attgtacgtg gccgcagtac gatcgacaaa agcatgatcc tattgaagat gcgttgcacg
     8461 ttaccgcacc actcgtcatc gtcgaaggaa actggctatt actggatgat gaaaagtggc
     8521 aggagttggc atctttctgc gatttttcta tttttattca tgcacctgct caaattctgc
     8581 gggagcgctt gattagtcgt aaaattgcgg gaggattatc gcggcaagaa gccgaagcat
     8641 tttatgcccg aaccgatggc cccaatgtcg agcgtgtgct gatgaatagt cgtcaggcca
     8701 atctgatagt agagatgact gaagaaggcc ggtatcactt cacaagctga caatttattc
     8761 cagttattgc agggttatta ttacgccagc ctggaataaa gcttcccaca aaaggggata
     8821 aaagtgtgat gtgagtcaga taaatgtctt ctttgactgg acaatcattc cttttattcc
     8881 acgtttcgct tatcctagct gaagcgtttc agtcgattaa atgttcgaca attaaccaat
     8941 cagtcgcagt ttgcgacagg taaggtttcc ccggacgatt tgctggatta ctctgtctgg
     9001 caaatttgct cagggaaaac cttgcaggag atctatgacc gtacgcgtag cgataaatgg
     9061 cttcggtcgc atcgggcgta atgtggttcg tgctttgtat gaatccggac gccgggcgga
     9121 aattaccgtg gtggcaatca acgaactggc ggatgctgcg ggcatggcgc atttgttgaa
     9181 atatgacacc agccatggcc gttttgcatg ggaagtacga caggaacgcg atcaactttt
     9241 tgttggtgat gacgccatcc gcgtattgca tgaacgttca ctgcaatcgc tcccctggcg
     9301 tgaacttggc gttgatgtag tcctcgactg caccggcgta tatggctccc gcgagcatgg
     9361 cgaagcgcat attgccgccg gggccaaaaa agtgctcttt tcacatcctg gcagtaacga
     9421 tctcgacgcg accgttgttt acggcgtcaa tcaggatcaa cttcgtgcgg aacaccgcat
     9481 cgtttctaac gcttcctgta ccacgaattg cataattccc gtcatcaaat tgttagatga
     9541 tgcgtacggt attgagtccg gcactgtgac cacaattcac tccgccatgc acgatcaaca
     9601 ggttattgat gcataccatc ctgacctgcg tcgcacccgg gcagccagcc agtcgatcat
     9661 tccggtcgat actaaactgg ccgccggtat cacacgattt tttccgcaat ttaacgatcg
     9721 ctttgaagcg attgcggtac gtgtgccaac cataaatgtg acggcaatcg atttaagcgt
     9781 gacggtgaag aaacctgtaa aagccaatga agtcaacctg ttgctgcaaa aagcagcaca
     9841 aggtgcattt catggtatag ttgactatac ggaattgccg ttggtctctg tagattttaa
     9901 ccacgatccg cacagtgcca ttgtcgatgg cacccaaacc cgggtcagtg gcgcacacct
     9961 gatcaaaacg ttggtctggt gcgataacga atggggcttt gctaaccgaa tgctcgacac
    10021 gacgttagct atggctactg ttgctttcag gtaagacgca agcagcgtct gcaaaacttt
    10081 tagaatcaac gagaggattc accatgtctg taattaagat gaccgatctg gatcttgctg
    10141 ggaaacgtgt atttatccgt gcggatctga acgtaccagt aaaagacggg aaagtaacca
    10201 gcgacgcgcg tatccgtgct tctctgccga ccatcgaact ggccctgaaa caaggcgcaa
    10261 aagtgatggt aacttcccac ctgggtcgtc ctaccgaagg cgagtacaac gaagaattct
    10321 ctctgctgcc ggttgttaac tacctgaaag acaaactgtc taacccggtt cgtctggtta
    10381 aagattacct cgacggcgtt gacgttgctg aaggtgaact ggttgttctg gaaaacgttc
    10441 gcttcaacaa aggcgagaag aaagacgacg aaaccctgtc caaaaaatac gctgcactgt
    10501 gtgacgtgtt cgtaatggac gcattcggta ctgctcaccg cgcgcaggct tctactcacg
    10561 gtatcggtaa attcgctgac gttgcgtgcg caggcccgct gctggcagct gaactggacg
    10621 cgctgggtaa agcactgaaa gaacctgctc gcccgatggt ggctatcgtt ggtggttcta
    10681 aagtatctac caaactgacc gttctggact ccctgtctaa aatcgctgac cagctgattg
    10741 ttggtggtgg tatcgctaac acctttatcg cggcacaagg ccacgatgtg ggtaaatccc
    10801 tgtacgaagc tgacttggtt gacgaagcaa aacgtctact gaccacctgc aacatcccgg
    10861 ttccgtctga tgttcgcgta gcaaccgagt tctctgaaac tgcaccagct accctgaaat
    10921 ctgttaacga tgtgaaagct gacgagcaga tcctggatat cggtgatgct tccgctcagg
    10981 aactggctga aatcctgaag aatgcgaaaa ccattctgtg gaacggtccg gttggcgtgt
    11041 tcgaattccc gaacttccgc aaaggtactg aaatcgtggc taacgctatc gcagacagcg
    11101 aagcgttctc catcgctggc ggcggcgaca ctctggcagc aatcgacctg ttcggcattg
    11161 ctgacaaaat ctcctacatc tccactggcg gcggcgcatt cctcgaattc gtggaaggta
    11221 aagtactgcc tgcagtagcg atgctcgaag agcgcgctaa gaagtaaaaa atcacagggc
    11281 agggaaacct gcccttgttt cagcgcgctt ttagagcacg cattttttca tactctaaat
    11341 aattcgagtt gcaggaaggc gacaagagag tgaatctcca ggagcttaca taagtaagtg
    11401 actggggtga gcgaacgcag acgcagcaca tgcaacttga agtatgacga gtataaggcc
    11461 cgacgataca ggacaagaga catgtctaag atttttgatt tcgtaaaacc tggcgtaatc
    11521 actggtgatg acgtacagaa agttttccag gtagcaaaag aaaacaactt cgcactgcca
    11581 gcagtaaact gcgtcggtac tgactccatc aacgccgtac tggaaaccgc tgctaaagtt
    11641 aaagcgccgg ttatcgttca gttctccaac ggtggtgctt cctttatcgc tggtaaaggc
    11701 gtgaaatctg acgttccgca gggtgctgct atcctgggcg cgatctctgg tgcgcatcac
    11761 gttcaccaga tggctgaaca ttatggtgtt ccggttatcc tgcacactga ccactgcgcg
    11821 aagaaactgc tgccgtggat cgacggtctg ctggacgcgg gtgaaaaaca cttcgctgct
    11881 accggcaagc cgctgttctc ttctcacatg atcgacctgt ctgaagaatc tctgcaagag
    11941 aacatcgaaa tctgctctaa atacctggag cgcatgtcca aaatcggcat gactctggaa
    12001 atcgaactgg gttgcaccgg tggtgaagaa gacggcgtgg acaacagcca catggacgct
    12061 tctgcactgt acacccagcc ggaagacgtt gattacgcat ataccgagct gagcaaaatc
    12121 agcccgcgtt tcaccatcgc agcgtccttc ggtaacgtac acggtgttta caagccgggt
    12181 aacgttgttc tgactccgac cattctgcgc gactctcagg aatatgtttc caagaaacac
    12241 aacctgccgc acaacagcct gaacttcgta ttccacggtg gttccggttc tactgctcag
    12301 gaaatcaaag actccgtaag ctacggcgta gtgaaaatga acatcgatac cgatacccaa
    12361 tgggcaacct gggaaggcgt tctgaactac tacaaagcga acgaagctta tctgcagggt
    12421 cagctgggta acccgaaagg cgaagatcag ccgaacaaga aatactacga tccgcgcgta
    12481 tggctgcgtg ccggtcagac ttcgatgatc gctcgtctgg aaaaagcatt ccaggaactg
    12541 aacgcgatcg acgttctgta agtcattcct ttctgcttat ctcaaggccc gctctgcggg
    12601 tctttttttc gccaaaagaa aaattgcctg atgcgctacg cttatcaggc ctacacgtat
    12661 cctgcaatat attgaattcg caggtttttg taggccggat aaggcgttca cgccgcatcc
    12721 ggcatgacaa agcgcacatt gatatctgtc tgagccccgc tctgcgggga ttttttcgcc
    12781 aaaagcaagg tgatacagat gagaaatctg tgatctattt ggcaaaatta tgctttattg
    12841 tttacccttg tcagactgcc cgtcataagg cggcggagtg tatttctcca ttttgagtca
    12901 gttgaaaagg aatattgaat ggaagatttg aatgttgtcg atagcataaa cggcgcggga
    12961 agctggctgg tggctaacca ggcgctgctg ctaagttatg cagtaaacat cgtggcggca
    13021 ctcgcgatca tcatcgttgg tttgattatc gcgcggatga tttccaacgc ggtgaatcgc
    13081 ctgatgatct cccgtaaaat cgatgccact gttgctgatt ttctttctgc attagtccgt
    13141 tacggtatta tcgcctttac gctaatcgct gcactagggc gcgtgggcgt acaaaccgcg
    13201 tccgtcattg ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt gcaggggtca
    13261 ctgtctaacc tggccgctgg cgtgttactt gtcatgttcc gcccgttccg tgccggagaa
    13321 tatgttgacc tcggcggcgt agccggtact gtgctgagtg tgcagatttt ctccaccacc
    13381 atgcgtactg cagacggtaa aattatcgtt attccgaacg gtaaaattat tgccggaaat
    13441 attattaact tctcccgcga gccagttcgc cgtaacgagt ttattattgg cgtggcgtat
    13501 gattccgata tcgatcaggt taagcagatc ctgaccaata ttatccagtc tgaagatcgc
    13561 attttgaaag atcgcgaaat gactgtgcgc ctgaacgaac ttggtgcatc gtcgattaat
    13621 ttcgtggtcc gcgtctggag caacagcggc gatctgcaaa acgtgtactg ggatgtgctg
    13681 gagcgtatta aacgtgaatt tgatgccgcc ggtatcagct tcccgtaccc gcaaatggat
    13741 gtgaacttta agcgggtgaa agaaaataaa gaaaacgaag ccgtaactcg ttaatcatgt
    13801 aaagcgccca tgaagttcat gggcgcacta ccaatttaac gcattgtttc atcaagtata
    13861 ttggcaactt tttgcatgac tcccccggct ttctccacgc cttcattatt ttgcttttct
    13921 acccactgag tttgctcttg ctgataacca atcgctttct gccaggcgtc cagctctgat
    13981 tttttcaaat agcgaacctg tgcaccttcc tgctcaagcg tttttaccat gctgatcagg
    14041 ttgttatcta atgccttgcc cagcgctttc tcggttgtaa tggccgctcg ttgaatagcc
    14101 tcacgatctc tgttatcaag gttttcccac gtttgtttat tcattaccaa cagataaaca
    14161 tgaccaagcc agagcgaagg ggagagcaac acattcggcg cagcacgttc agcatggatg
    14221 tcatatccgc tatcaagatt gaccattaag ccatcgagtt gtccgtcgcg gagtgctgtc
    14281 gtaatttgct cattccacgg taaggttacg gtttttgccc ccgtatgagt taaataagcc
    14341 cgatgccaga aactggctgt tcgccaggtg gttccctgaa gtgcagtcaa tttatcaatg
    14401 ggcctggtag agaaaaagcc taccgggtag ccaaggaaaa actgtaaatt cacgatattg
    14461 ttacgctcaa gttcagcgtt aaattcggga atttccgcat atacgcgacg aaagaattct
    14521 acctgactgg ctccatgatc cgggccaata gcaaagctct tgaagatttg atgaagcggt
    14581 aattgtttcg ctgtgtattc cggtaccact atacccatat cagctttgct accatcactt
    14641 attgtcgtca gagcgtcata gctgatggcg gtttcaccgt tccagtgtgg ttcgattttc
    14701 aaacgcccct gcgactcttt ttcaatttct gcaaaaaata tctctttaat tatttgtgta
    14761 cgcattcccc cgtagggttc atggtctgta taacgaagaa ttttttcagc ggcaacgttg
    14821 tgggaagata tcagaagtaa tcctgatacc agagaaagta aaaagtgttt gaatgtcatc
    14881 tcagttccat ctgtaatttt attggtcgtc attaatatta aaagccgtga actttaacaa
    14941 ctaaaatcga acttcatatt tttaaagtcc agccacgcat tgccagtacc aaaattatac
    15001 ccgccgaagt attcttcaaa ttcaatataa aaaggtgaca ttccatctgg tatagattgc
    15061 aatggaacca ttgaaccacc gagatgatcg ggttgatgtg cactatgcca ctggtgagtt
    15121 tcacatttac tctttccctc tgcatcttta gaaaaataac gaatggactg atattccgta
    15181 ttatttcttt ctgtggggct aattcccgca ttaggatcaa aagcgcgcgg cttacatgaa
    15241 atcgccagat tgaaagccag cgactgctca ggaatggtgc ccattttacg atatacaaaa
    15301 ttgctttcag gcgtatcttt tggtctgacg cttgcctgaa aataactaaa cgcactgccg
    15361 gtggtcatcc attctggtgt atcttgctgg ttgcgatagt gattaggtat aagttcaggc
    15421 ggttcacaaa atgggccgtt gaactcttgc tgggtgagta aaatcaccgc ataagaacaa
    15481 cctaatatat cttccatccg aaatagccgt ggatgtcgtt gcttttctgc cagccagaaa
    15541 acataaaggt ctggatcttc ggggggtatt ccggactcaa aattctcaaa cacatccaat
    15601 aattcttcta tacatggtgt tccgccatcg ttgtgctcgg gcgcaacagc aaagaaagat
    15661 aacgcgacaa tctctggccc ataaatccga taatcttccg gcaacagcag ggtaaatcca
    15721 tgattcaatg ggaaaccatt atttggatcc agcggccact gttcgggcgt tatgcctggc
    15781 ggtaggccaa agcaccaacc ttgttggcgc ggcatatttt cgcggatagg atggggcaag
    15841 agggtaagat cataagcatg cattgtgtga gtcctttata aagcgaggcc gtaatggccg
    15901 tagcacgatt cctgtgagcg atattgttat ccaaaaaata gccacgcatc ctgactgttc
    15961 gttttcttat taatattatt aatatgctat taaaaatatc catttcctct aatgatgctc
    16021 acacgctata gtctgcaaat cagataatta gctcggaata aattacgtga tatcttatta
    16081 ctttcaaggt cttgcacttg gcgcagctat gatcctaccg ctcggtccac aaaatgcttt
    16141 tgtgatgaat cagggcattc gtcgtcagta ccacattatg attgccttac tttgcgctat
    16201 cagcgatttg gtcctgattt gcgccgggat ttttggtggc agcgcgttat tgatgcagtc
    16261 gccgtggttg ctggcgctgg tcacctgggg cggcgtagcc ttcttgctct ggtatggttt
    16321 tggcgctttt aaaacagcga tgagcagtaa tattgagtta gccagtgccg aagtcctgaa
    16381 gcaaggaaga tggaaaatta tcgccaccat gttggcagtg acctggctga atccgcatgt
    16441 ttacctggat acttttgttg tactgggcag ccttggcggt cagcttgacg tcgaaccaaa
    16501 acgttggttt gcgctcggga caattagcgc ctctttcctg tggttctttg gtctggctat
    16561 tctcgcagcc tggctggcac cgcgcctgcg cacggcaaaa tcacagcgca ttatcaatct
    16621 ggtagtggga tgtgtcatgt ggtttatagc cttgcagctg gcgagagacg gtattgctca
    16681 tgcacaagcc ttgttcagtt aggcgctatc tgatggaaaa ataaaacaga ggcgctaagc
    16741 ttgcctccag aggtcctgaa ttttgggcaa gtcaattaac acggagagac taacgtgaag
    16801 ttcaaagtta tcgccctggc ggcattaatg ggtattagcg ggatggcagc gcaggctaac
    16861 gaattgccgg atggaccgca tattgtcacc tccggtacgg caagcgtgga tgcggtgcca
    16921 gacattgcca ctcttgcgat tgaagtcaac gtggccgcga aggatgccgc tactgccaag
    16981 aaacaggcag atgagcgcgt cgcacaatac atttccttcc ttgaactcaa tcagatcgcg
    17041 aaaaaagata tcagctcagc gaacttacgt acccagccag attatgatta tcaggatggt
    17101 aaaagtatcc tgaaaggcta ccgcgctgtg agaacggtgg aagtcacgct ccgtcagtta
    17161 gacaaactga attccttgct ggacggcgcg ctgaaggcgg gtcttaacga aattcgttct
    17221 gtgtcgctgg gcgtggcgca gccggatgcc tataaagaca aagcgcgtaa ggcagcgatt
    17281 gataacgcga ttcatcaggc gcaggaactg gcgaacggct ttcatcgtaa actggggccg
    17341 gtatatagcg tgcgctacca tgtttccaac tatcagccca gcccaatggt gcggatgatg
    17401 aaagccgatg ccgcgccggt gtccgcccag gaaacttacg agcaggccgc tattcagttt
    17461 gatgatcagg tcgatgtggt cttccagtta gaacctgtgg atcaacaacc cgctaaaaca
    17521 cctgcagcac aataatactt gtagcccgac gtattcgtcg ggctaatgcc tttacccgat
    17581 attgcctcct ttttctgtaa ttttctgtaa aaccatgacg taatcaccta tctttgctcg
    17641 cggtttcatc aaggataggc tgactgaaac ggttaagcgg acaaggatgc gggggagggg
    17701 ggatggacat ttttatttcg aaaaagatgc gcaatttcat tttattagcg caaaccaata
    17761 atattgcccg ggcggcagaa aaaatccata tgacggcttc gccatttggt aaaagcattg
    17821 ccgcgctgga agaacaaatt ggctatacgc tatttacccg caaagataat aatatcagcc
    17881 tcaataaagc cggacaggaa ctgtatcaaa aactgttccc ggtttatcaa cggctttctg
    17941 cgattgataa tgaaatccat aactcaggac gtcgttcacg agaaattgtg attggcatag
    18001 ataatactta tccgacaatt attttcgatc agttgattag tctcggagat aagtatgaag
    18061 gcgtgaccgc ccagccagtt gagttcagcg aaaacggcgt tatcgataat ctttttgacc
    18121 gccagttaga ttttattatc tccccacagc atgtatctgc acgagttcag gaactggaaa
    18181 acctgactat cagtgaacta ccaccgctgc gattgggctt tctcgtttct cgccgttatg
    18241 aagagcgtca agagcaagag ctgcttcagg agctgccctg gctacaaatg cgctttcaaa
    18301 accgggctaa cttcgaagca atgattgatg cgaatatgcg accttgtggg atcaatccaa
    18361 ccattattta tcgtccctac agttttatgg ccaaaatcag cgccgtggag cgcggacatt
    18421 ttctgacggt gatcccgcat tttgcctggc gactggtgaa tccggcaacg ctgaaatatt
    18481 ttgatgcgcc gcacaggccg atgtatatgc aggaatatct ttattcaatc agaaatcatc
    18541 gttataccgc cacgatgctt cagcatattg ctgaagatcg tgacgggaca aaccattaac
    18601 ccagcatcga gccggttgca attaaattac ggtgtaagtc gaagacgtgg ctaagatcgt
    18661 ggtgaatatg tccgccaggc gcattttcca gatagcgatg cagataatcc cgatacatag
    18721 gatgtgcaca attatcaatg atcgtgcggg cgcgttgaag cggggaaaga ccgcgcagat
    18781 cggcgatccc ttgttcggtg atgatcactt tgacgctgtg ttcgctgtga tcaacatggc
    18841 tgcacattgg cacgacggtt gagatcttgc cttctttagc aatcgatggg gccataaaga
    18901 tcgacagata cgcgttgcgt tcaaaatcac cgctgccgcc gatgccgttc atcagatcga
    18961 ccccggctac gtgcgttgag ttggcatgcc cgtaaatatc aaactccagg ccgacgttca
    19021 gagcgatgac gcccagacga cggatgattt ccgggttatt ggaaatctcc tgcggacgca
    19081 acacaatgcg gctggcaaag taatccatat tgtcgtaaat cttgcgcagg gaatcggccg
    19141 agattgtcag gctggaggcg ctggccccgc tgattttgcc ggtttccagt aaatgcacca
    19201 ccgattcctg tagcacttcc gaatacatca taaacggagg aatttccggg ttttccccca
    19261 gacgcgccat taccgcatta ttgatattgc ccacgccact ttgcagcggc agaaattccg
    19321 gcggaatacg cccatgcgcc atttcctgca ataagaacgt gaccacgtta tcggcaatct
    19381 gctggcacat gggattttgc ttatccagca tattaccggc gtcaggcaag ttggtttcca
    19441 cgacggcgac aatctttttc ggatcgattt gcacatagcg ggtaccgacg cgatccattg
    19501 catggaagat cgacacgcta ttgcgccgtg gtggcgcgcc aggaatcaca atatctgcca
    19561 gttctgcaac gcgcggatcg tgatagtgat tgagttcaat gatcactttc ttcgctcgca
    19621 gcagccaggt cggcgcatta ccgatcccgc tggttaacca gactcgacca tctggtgcca
    19681 gtgccgatgc ttcaatgacg gcaacatcaa tgtcgccgaa gaaaccgtaa ttgaccattt
    19741 gcgccacttc gctcaaatgc aggtcaacga aactcaccgc gccctgattg atctttttac
    19801 gtaaaccgga cgatgtttga tatggcgcac gccaggaaac agcatcggcg tcagaaagta
    19861 catcgtcagc ggcggcgctg attgacgccc ccgtcagcag gcgaatttga tacggctttt
    19921 tggcctcatg ctgttcgtta gctctgcggg caatcgcggt gggtagggct ttcggcgaac
    19981 ccgccggggt aaagccgcta aatgccacca tgtcgttatg ctggataatt tctgccgctt
    20041 catcggcggt catccttgtc cactgagttt ccatttttac tccttcgttc gcagggatta
    20101 atgaccaacg aaattaggtt tacgtttttc gaggaaagcg ttcatccctt cctggtaatc
    20161 ttcgctgtca taaaccgcgc ggcgcatccc ctgaatacgt tcaaattcat cggagttcat
    20221 ggtgtgtgct tcgcccagta cacgcagctc ttctttgata acggcaatgg ctaacggcgc
    20281 tttctcagag atgtggtgcg ccatttgtaa ggtgaaatct tccagttctt ccacttccac
    20341 aacatggttg aggatgccga cagccagcgc gcgctgggcg gtgattggcg aagcggtaaa
    20401 aatcagctct ttgacaatgt ggaagcccgc gtcgcgggtc aggttgtgaa tgccgaccag
    20461 gttatacggg acgccgaggt ttacaggcgt cattgagaag gttgaggtac tggcggcgat
    20521 gatcagatcg gaactcatga tcatttcaaa tgcgccaccc caaacactac cttccaccat
    20581 cgaaatgatc ggtttcggga atttttggat catgcgggtg atttgacgca atggatcatc
    20641 ataggagagc ggatcgcgac cgccagacgg cagttcgtga atatcgtgac ctgcggagaa
    20701 gactttggct ccactcggcg cgcgcaaaat gatacagcga atttccggcc ggttgagatc
    20761 gcttaacgcc tgcataagat catcaataaa gactttactt aaggcattaa gttttcggcc
    20821 atagttaaac tcaatgaccg ccactttgtt gatagtggca acgttcacat actgataaga
    20881 cataaaaatt cctttaatca aaatattgcg tctggataaa ttcactgagc tgccgcaggc
    20941 cggtgcgcgg tgagagcgta ttgtttttga ccgctaaaag cgtctggcgg taatagcgat
    21001 cgaaatcttc attcgcgaac aggtgattca gtacttcttc ttcggtctgc ttacgcagcc
    21061 attccaccga ttgttgttgc cgcacttgtt gtaaacgacc actggcagtt agcgcggttt
    21121 tgaagtcgat gatggcgtgc cagatctcat cgattccacg tttttccagt gcgctacaag
    21181 tcagaacccg tggctgccat tcgtcgtatt tacgtcgcag aatatgcagg gcactctcgt
    21241 acatatgccg ggcaatggcg acattggtat ggttatcgcc atcgtctttg ttgataacga
    21301 tcagatcagc cacttccatc agcccttttt taatgccctg cagatcatcg ccgccaccgg
    21361 caatttgcaa cgagataaaa cagtccacca tgcgggcgac ttccgtttcc gactgcccga
    21421 cgccaaccgt ttcgacaatc actacgtcat aacccgccgc ttcgcataac agcattaatt
    21481 cccgcgctcg ctgactggca ccgcccagat gaccggagga tggtaccggg cgaataaacg
    21541 ccgtctcggc acgcgccagg tcattcatgc gggttttatc cccgagaatg ctaccgccag
    21601 tgaccggact gctgggatcg accgcaataa ccgcaacctt taatccatca cgaatcaaca
    21661 acatgccaaa ggcttcaaga aaggtacttt tccccgcgcc gggtgtgccg gtaacgccca
    21721 gtcgcagggc gttaccgcag tacggcataa tggcatcaag cagttgcgta cttagcgcct
    21781 gatgacgcgg gtgacggctt tccaccagcg tcatggcctg ggcgagtgtt gcacgctcac
    21841 cctgacgtaa gcggcgaata ctttctgcca gcgtggcttc attaatcatg atgctggctt
    21901 atcagattca gtacgtcgcg cacactgtcg agcataggtg tacctggacc ataaatcgcc
    21961 gccacgccgc gctcttgcag gaaggcgtaa tcctgcggtg gaatgacgcc accggcgact
    22021 acgcagatat cttcgcgtcc ccattttttc agcgcttcga ccagttccgg gatcagcgtt
    22081 ttatgaccgg cagccagtga ggatgcgccc actacgtgaa cgtcattttc tactgccagg
    22141 cgggcgatct cttcaggtgt agagaacatc gggcttaaat ctacgtcgaa accgagatcg
    22201 gaataggcgc tggcgatcac tttcgcgccg cgatcgtgcc catcctggcc cattttggcg
    22261 atcagaatgc gcgggcgacg accattgtcg gcaaggaact gctccgtttg cgcaacaatg
    22321 gcatcgaact cggaggccga tttctcagat tgatgatagc tttgcgcaat cacgccggta
    22381 acacactggc ttggcaccag ataacggtcg aaagcggctt ccagcgcatc ggaaatttca
    22441 cccagggtgg cgcgaacgcg agcggcatta acagcggcag ccagcaggtt ttcgttatgc
    22501 tgtgcggcgt gagtcagggc gttcaacgcg gcggttacgg cggcatcatc acgggtggcg
    22561 cgaatgcgtt ccagcgaagc aatttgctcg ttacgcacca tcacgttgtc gatctcaagt
    22621 acatcggttt cgtcttcgtg atccagtttg tacttgttga caccaacgat gacacgcttg
    22681 ccctggtcga tcagcgactg ttcgcgcgct gaggcctctt cgatcattcg ttttggcaga
    22741 cctgcttcga tcgctttcgc catgccaccg gcttcgtcga tctgttggat aatagctctg
    22801 gcttgtttga cgatttgatc ggtcagcgac tcaatgtaat aggatccggc cagtggatcg
    22861 acggtgcggc agagttctga ttcttcctgg atgatgatct gggtgttgcg ggcaatgcgt
    22921 gctgagaaat cggtaggcaa accaagcgct tcgtcaaagg cgttggtatg cagtgactga
    22981 gtaccgccca gcgtcgcagc cagtgcttca atggtggtgc ggataacgtt gttatacgga
    23041 tcctgttcag tcaggctcca gcctgaggtc tggcagtggg tacgcagcgc cagtgatttc
    23101 gggtcctgtg cgccaaatcc actgaccgct tcgctccata aataacgtgc cgcacgcaac
    23161 atggcgacgt tcataaacag atccatgccg atgccgaaaa agaacgacag gcgaggagcg
    23221 aagtcatcaa ttttcagtcc ggcagagatt gctgctttga tgtactcaat cccatcagcg
    23281 agcgtaaatg ctacctgctg cacgcagttg gcacccgctt cacccatgtg gtaaccgctg
    23341 atactgatgg tattaaatcg cggcatgttg ccggaacacc aggcgatgat gtcggcgata
    23401 atgcgcattg acggttttgg cgggtaaata taggtgttgc ggcagaggta ctctttgagg
    23461 atatcgttct gaatggtgcc ggtcagttta tcaggtgtaa caccttgctc ttccgcggcg
    23521 acgatataaa acgccagtac tggtagcact gcgccattca tggtcatcga aaccgacatt
    23581 ttatccagcg ggatctggtc gaacaggact ttcatatctt ccacggtgtc gatagcgacg
    23641 cccgctttgc cgacgtcgcc cgccacgcgc gggttatcgg agtcgtagcc tcggtgggtg
    23701 gcaaggtcaa acgcaacgga aagacctttt tgcccggcgg ccaggttacg gcgataaaaa
    23761 gcgttggact cttttgctgt tgaaaaacca gcatactgac ggatggtcca cggttgggcg
    23821 gtatacatag tggcacgcgg gccacgaacg tagggcggca aaccaggaag ggtacctgtc
    23881 acctccagat tatcgagatc ggcttcggta tacagcggct tgatggcgat cccttccgcg
    23941 gtttgctgaa ccagcgagtc gacagttttc tcccgacggc tcaattcctt gttggcaagc
    24001 tgttgccact cctgcacgtt agacattatt tgctccgtaa aacgcaatca aaatgagggt
    24061 atttgcgcct acggtgagct ttttacgtgg gtttgtcgcc agcaaaaaaa atgcattcag
    24121 acgtcgtttt ttgccgcctg atatttgtct attttgtgat gtgattcaag gaatacaaaa
    24181 agaagtgagg acaacgcctg atatgtgcta tcaggcatta tttgatggat taatcctgac
    24241 gaaggacttt gtgaccataa tcgagtaacg catcagtgac tttacgcatc atgcggcttt
    24301 caggagcaaa gcggtgccag tagagcatcc gtcgttgaaa tagcccaggc gttaagtcaa
    24361 tcagttcacc gctggccagc tctttctcga tttgcaggtg cgggatcata cagcaggtgg
    24421 tgccctggcg agcaagttgt acgaacgctt ctgaagaatt aacgatatgg cagggcacgc
    24481 tgcctggagg cagatcgaag ttttgctgca aaaaggcctg gtgcatatcg tcaagatggt
    24541 caaacgcgac cactggcgct ttcagtaatg ccgaacgcgt tacgccgtta gggaaatatt
    24601 tttcggcaaa gggttttgag ctgacgaaca gatagtcgag tgcaccaagt ttatcgacaa
    24661 gacaactcgg cagcgcctga tgttgaatac tcaccgcgcc gaccacttcg ccgcggcgca
    24721 gacgttcctg agtgcgggtt tcatcttcta cctgcaagtt gaggcggata ggcgaatcag
    24781 ccaacacagg agccagtgca ggaagcaacc acgtcgccag actgtcggcg ttgaccgcca
    24841 gtgaaagcag cagcggagtc gaaccggttt gttcatcgcc cagccactct tcttccagca
    24901 actccacctg gcgcagcagt gccagcagtt tttgcccttg ttccgtcggg cgcggcggta
    24961 cggtacgcac caacagcggc tgcccgaaca tattttccag ttgcttaatg cgctgtgaga
    25021 cggctgattg tgtaatgcac agcttttgtg ccgcgcgctc aaatcctcgt tcacgtatca
    25081 ccgcatccag tgcctgtaat gttctgtagt ccgggcgttt cattgctgtt gtctgctcct
    25141 ggtaaatgct ttttcctgca ctatgacaca attttgcgtt gggtacagag catttaacgc
    25201 atacaaaagc ggtcgtaatg gaagttttgg ctggcaatcc cattttcata taaccctttg
    25261 ctgagcgctt tggcaaagct ctgtgggccg caaaaccaga aatgggtgtc acctttattt
    25321 gctattttat cagtaagata gtgcgactgt aggcgctcac ctttatgcgc atcaagcaga
    25381 tgcagtgtga cactggcctg ggcacaaagt gaggtaagtt tatcaacata ccatgcatcc
    25441 tcgcttcggc tgacgcaata ccagagatcc acaggaacac gggacgcgcc atgctggttc
    25501 agatactcaa gctgggcgat aaaggggtaa tgccgatgcc tccagctatc catacctggc
    25561 gggagcaagg gagagtaaaa tcaaactttc cccatggccc ttcaacgaga gcggactggc
    25621 cgattgttat cgaggatgcc agagtcgagg tataatcccc taaatttttg atggcaaagc
    25681 gcaacgtgtt cttctctatt gcatatgaag cgatagtaaa agggtgagac tctttactgt
    25741 gtgcgaacgt taagaatgca aactgacccg gagaatagtc gatgacatgt tccagctcca
    25801 gtatgacttc ggtaatttgt cccggatggt taacaatact tttaatggtg gcctgatgct
    25861 tgtttttatt accgtttaat ttcagcagcg aaatgaaagc cgcaaagacg cctgggatag
    25921 taattaaaat taataaatac gcagcaggag tttcccacca atatgattta aacagtgcag
    25981 taaatacatg aaaagcaatt gccagataaa aaacggggaa caaacggtga acaagatgaa
    26041 ataaatgata gggaactttt ttgcttagag acacgaaaac catcgctatc ataataaaca
    26101 ttgcacattc acccgctaac aatcccgcat gaagccaatt ttcttgtact ggcgtcaggt
    26161 taatatcaac gattttacca gggtgagtaa gccagccaaa ggcgacgaat aactggggta
    26221 atttttcatc cagccagtgg gcgaaagcaa aagcgatggc gcatattcct gcccacttat
    26281 gtaatcgcca tgctttatcc agaccgccca gcactttatt aagccatgat gaacgcagcg
    26341 ataaacacat cgtcagcgtc atcagacacc acgcaagggt tcccgtaaaa aagagcgtgt
    26401 tttgtcgaat gaaccatatt agcgcgattg gcttttcacg aaaaatatcg ctggacgaca
    26461 aaaaaataat acatgcgaca aagcaaacca acgtacctgt aagattagct tttttcattt
    26521 tttatttcat tttggcagcg gagcatagaa cagttccatg ctcccgttaa atagagtgat
    26581 tatttataga aaatcacagt ggcgtgaata taatttttac cggagaaaga acttatataa
    26641 taattgctgg cttgtaactc ttccgctttc tctttgattt ggctttcaac gccttctggg
    26701 ttgctattgt gtgtagacat gctcactttt gataactgtt caaggttatt gtccggttta
    26761 tgtatctcgg ttgcggcaaa tgcgtgtcct gtgagagtgc aaagaaataa cgcggcaaat
    26821 aaattttgct taatagacat aataactcct tttatattaa agataatgat taaatacttg
    26881 tgtaataagt tcagggattt gtgctaatgc attttgctcg gcgagttcgc gtcccggttg
    26941 ggttttgtcg acgccttccg cacgtaaaat atgtgtattg gtaatgccca ttaatttcag
    27001 cgtggcttgc aggtaacttt cctgatgatc cagcgccaga tgtgcttcat cgcaatagat
    27061 accgccgcgc gtcgagacaa taaacgtcgg tttatccgtc agtaacccaa cggcaccagc
    27121 ggaagtaaaa cgaaacgtta aaccagcctg acagacctga tctatccagg ttttgaggcc
    27181 agatgaaacg ctgtgattaa tcatcggcgc gccaataatg agcacatcgc acgatgccag
    27241 ttcttcaata gcggcgtgaa tcccattcac ctcctgccga atttgtgcgc tcccgcactc
    27301 tgctttatcg caaagcgcca tgaaggtttc ttcacgtaaa tggggcggcg gcgtcaggcc
    27361 aacgtcgcga tgaataattg tggctttcgg aaagtgttgg caaagcgctt ctaccagcgt
    27421 ctggcttaat ttccgcgaac ttgaatgctt aagcattggg ctactgtcga tatggagaat
    27481 tttcatgggc ttgctctcgt ttctgcgatg agagaagttt aactgttgtt gatatgatga
    27541 ttaaatagac ataattgaac tttattgttg tgaaaataga acaatcatga tcgactggaa
    27601 tgattattac tactttgccc tgattgccga tgaaggcagc tacagccagg cggcgctgcg
    27661 tgcgggcgtg tcgaaatcgg tattaagccg acgaatggct gcgctggaag agcgccttgg
    27721 cgtacggctt attcagcgaa ctacgcggcg tctggccctg acggcggcag gcgaacagtt
    27781 tgccgtcgaa tgtcgggcgc tggtggcgca gggggaacgg gccagagcgg tggtgatggc
    27841 cgcccaggaa agcccacagg gaatgctgcg cgtcagcgcg ccggtgttta tggctgaaac
    27901 atggtttggt gagtttgtca gtgagtttat cgcccgttgg cccctggcga aggtgcaact
    27961 gctggcaatt aatcgcccgg tggatatggt cgctgaaggc attgatattg cgctggttac
    28021 ggcttccggc gcgcttgcgg actcgacgct gcattataaa aaattacata cccaggccga
    28081 tattttagtt gccagttcgc agtggctggc ccgccatccg caggtgaagg ccattgacga
    28141 tcttaaccgc gtcgacggga tccttcgcct gacaaacaat ggcgggagcc agtggcagtt
    28201 aattaatggc gaacaggaga ttacgttgca gcctgaggcg cgtttgcaga cgaataacat
    28261 tcgcgtcatg ttgcaggcag tgcttggtgg gaatggcgtg gcgttattgc ctcaatttgc
    28321 ctgtaaacag gggcttgcga acggtgaatt ggtgcgcctg tttgcaccgt ggagcggcat
    28381 acccagacca ttgtatgctt tatttgcggg gcgaaagggg atgcctgcca ttgcgcgata
    28441 ttttatggat gagttaacca cgcggcttgc caacggggtc tgaatcgctt tttttgtata
    28501 taatgcgtgt gaaatttcat accacaggcg aaacgatcat gacgcaggat gaattgaaaa
    28561 aagcagtagg atgggcggca cttcagtatg ttcagcccgg caccattgtt ggtgtaggta
    28621 caggttccac cgccgcacac tttattgacg cgctcggtac aatgaaaggc cagattgaag
    28681 gggccgtttc cagttcagat gcttccactg aaaaactgaa aagcctcggc attcacgttt
    28741 ttgatctcaa cgaagtcgac agccttggca tctacgttga tggcgcagat gaaatcaacg
    28801 gccacatgca aatgatcaaa ggcggcggcg cggcgctgac ccgtgaaaaa atcattgctt
    28861 cggttgcaga aaaatttatc tgtattgcag acgcttccaa gcaggttgat attctgggta
    28921 aattcccgct gccagtagaa gttatcccga tggcacgtag tgcagtggcg cgccagctgg
    28981 tgaaactggg tggtcgtccg gaataccgtc agggcgtggt gaccgataat ggcaacgtga
    29041 tcctcgacgt ccacggcatg gaaatccttg acccgatagc gatggaaaac gccataaatg
    29101 cgattcctgg cgtggtgact gttggcttgt ttgctaaccg tggcgcggac gttgcgctga
    29161 ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc tgacggggga acctcccccg
    29221 ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca cgtttttacc tggctattgt
    29281 cgattgctct aaataaatcc tctaaaccag catattcatc caagaattac ctttgcgtga
    29341 tatttcctca acatcgcgac gcaaacgttc atattgccgc aatattattt tttgatatgt
    29401 tgaaaggcgg acgcaaatcc gcacacaaca tttcaaaaga caggattggg taaatggcaa
    29461 aggtatcgct ggagaaagac aagattaagt ttctgctggt agaaggcgtg caccaaaagg
    29521 cgctggaaag ccttcgtgca gctggttaca ccaacatcga atttcacaaa ggcgcgctgg
    29581 atgatgaaca attaaaagaa tccatccgcg atgcccactt catcggcctg cgatcccgta
    29641 cccatctgac tgaagacgtg atcaacgccg cagaaaagct ggtcgctatt ggctgtttct
    29701 gtatcggaac aaaccaggtt gatctggatg cggcggcaaa gcgcgggatc ccggtattta
    29761 acgcaccgtt ctcaaatacg cgctctgttg cggagctggt gattggcgaa ctgctgctgc
    29821 tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg tggcgtgtgg aacaaactgg
    29881 cggcggggtc ttttgaagcg cgcggcaaaa agctgggtat catcggctac ggtcatattg
    29941 gtacgcaatt gggcattctg gctgaatcgc tgggaatgta tgtttacttt tatgatattg
    30001 aaaacaaact gccgctgggc aacgccactc aggtacagca tctttctgac ctgctgaata
    30061 tgagcgatgt ggtgagtctg catgtaccag agaatccgtc caccaaaaat atgatgggcg
    30121 cgaaagaaat ttcactaatg aagcccggct cgctgctgat taatgcttcg cgcggtactg
    30181 tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa acatttggcg ggggcggcaa
    30241 tcgacgtatt cccgacggaa ccggcgacca atagcgatcc atttacctct ccgctgtgtg
    30301 aattcgacaa cgtccttctg acgccacaca ttggcggttc gactcaggaa gcgcaggaga
    30361 atatcggcct ggaagttgcg ggtaaattga tcaagtactc tgacaacggc tccacacttt
    30421 cggctgtgaa cttcccggaa gtctcactac cactgcacgg tgggcgtcgt ctgatgcaca
    30481 tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa aatctttgcc gagcagggcg
    30541 tcaacatcgc cgcgcaatat ctgcaaactt ccgcccagat gggttatgta gttattgata
    30601 ttgaagccga cgaagacgtt gccgaaaaag cgctgcaggc aatgaaagct attccgggta
    30661 ccattcgcgc ccgtctgctg tactaattcc ccttctctga caatcaacgg gcgtcactga
    30721 cttgcccgtt tttttatccc ttctcccgcg catgtcgctg ccatttgtag tgcgctgcaa
    30781 tcgtctttgc gagtctgctc gaagaaataa atcaaagtca gatcaatgcg ttgtgtttat
    30841 ttaatgagga tttacgatgg cggagcagca cagggctgta acggtaaagc cctcaccgaa
    30901 gcgagggctt aaaggagaag ggttatgatg tgacttgtca tcatactgat tgtactgtta
    30961 ctcataagtt tcccagctta ttaacagtca gtcacagggg aggtgcaatc ctcccttacc
    31021 cttactcact aaattaggtt aaataatcaa cgatgtcaat cagggcgatg caggggtgtc
    31081 gccctcacca ctcccagatt ttcgatggcg taaccaccgc aggcagcggg atgtcccatt
    31141 cttctacggg gagtttttcc actaactggc aatcatgcgc ataacccacc ggttgcattt
    31201 tatagtgctg ccagttttgt aaggtccgat cataaaaacc accgcccatt cccaggcgct
    31261 gaccgtactc atcaaaggcg accagcggtg tgatcaacac gtctaatcgg gaaaggggga
    31321 gcacgtcacg cacatccagt tttggctcat ggatcttcaa cctgttcatc accagttcac
    31381 tttgcggatg gtaattcagg aacagcaaat taccggcact aaagggatgc aaaactggaa
    31441 gatatacgcg cttaccggcg cgccagagtt gttctatgag tggttgggtg tcgagttcgc
    31501 catcaaaaga gaggaataca gcgaccgtat gtgccatcac caccggggga taagtcatca
    31561 tccgggtagc ggcttgttga cccatttcct gctgttgttc cggcgttaac gcacgacgac
    31621 gttgccgaat cattttgcgg atttcttgtc gggataatgt cagtgggagt tctggtagtt
    31681 gcgtcatggc tggtgccaga taagaaggga atctccgaga tgccgccgca ggctgtaacc
    31741 cttgaaccct tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct cggacggacc
    31801 gagcatgctc accaaccgcg gagcgccaca ttcttgtggt atgaaatatc ggctcagggg
    31861 actggcccgc ttgcgaacat ctcagagaaa ttttgtcttc acggttactc taccacagta
    31921 aaccgaaaag tgtcattcaa agttttggtt agttttttcg gtgatgcgac cttgttcaag
    31981 taacgcttgt tctatggtct gctgcagcat ccgaatacgc tgttccatac ttgccgcgta
    32041 gtcacgagtc ttcgcttttt cttgcgctaa ctcatagctg atattcaatg cggcaatgaa
    32101 gaccaactgt tcagtatttg tgactctagt gcgttctttc agatcttgca accgttggtt
    32161 cagatcgtcc gctgcctgat tcaacgcatc cctttggtca ggcgggcagt tcacacgcag
    32221 tgaacggcca aaaatttgga tatcgacggg ttgtgcagac atgccacctt cctgctgatt
    32281 gactgcgctg ccttcgtctt tagacccatg gtctgcgaag gggcgacact atagctaccc
    32341 tgatgagaag agacaagccc ttttctggtc caccagggcc caaagtggta gcatatcatg
    32401 aatattcctc cctttgacga cgaatgctta tgtctataca gaacgaaatg cctggttaca
    32461 acgaaatgaa ccagtatctg aaccaacaag ggacgggtct gacccctgct gagatgcatg
    32521 gtttaatcag tgggatgata tgtggcggta acgatgacag ctcatggctg ccgctacttc
    32581 acgacctgac gaacgaaggc atggctttcg gtcatgagct ggcacaggca ctgcgtaaaa
    32641 tgcactctgc caccagcgat gctctgcagg atgacggctt cctttttcag ctttatctgc
    32701 ctgatggcga tgatgtcagc gttttcgatc gggctgatgc gctggccggt tgggtcaatc
    32761 acttcctgct tggtcttggc gttacgcaac cgaagctgga caaagtgacc ggcgaaaccg
    32821 gtgaagccat cgacgatctg cgtaacatcg cgcagttggg ctacgacgaa gacgaagatc
    32881 aggaagagct tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt
    32941 tatgccacga cacctttact catccgcaac cgaccgcgcc agaagtacaa aaaccgactc
    33001 tacactaaaa acaaaaaacg taaggagagt gttatgagtg agatatcccg acaagagttt
    33061 cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc gctgattttt
    33121 gctgcaccag aagtaacacg tagcgccgac agcgaatacc cctatcgtca gaacagtgac
    33181 ttctggtact tcaccggctt taacgaaccg gaagcggtgc tggtgctgat taaaagcgat
    33241 gacactcata accacagcgt tctgtttaac cgcgttcgcg acctgacggc ggagatctgg
    33301 tttggccgtc gtttaggtca ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg
    33361 gcattcagcg aaattaatca gcaactttat caactactta atggtctgga tgtggtttac
    33421 cacgcccagg gcgaatatgc atatgctgat gaaatcgtga acagcgcgct tgagaaactg
    33481 cgtaaaggtt cgcggcaaaa tctcaccgcg ccggcaacga tgatcgactg gcgtcctgtt
    33541 gttcatgaaa tgcgcctgtt caaatcacca gaagagattg ccgtacttcg ccgcgcgggg
    33601 gaaatcaccg ccctggcaca tacacgggcg atggaaaaat gccgtcctgg gatgttcgag
    33661 taccatctgg aaggcgaaat tcaccacgaa tttaaccgcc acggcgcgcg ctatccttcc
    33721 tataacacta ttgtcggcag cggtgaaaac ggctgcattc tgcactacac cgaaaacgag
    33781 tgtgaactgc gtgacggaga tctggtgttg attgatgcgg gttgtgaata caaaggttac
    33841 gctggcgata ttacccgcac cttcccggtc aacggcaaat tcacccaggc tcagcgtgaa
    33901 atctacgaca ttgtgcttga gtctctcgaa accagcctgc gcctgtatcg tccgggaact
    33961 tccattcagg aagtcaccgg tgaagtggtg cgcatcatgg ttagcggcct ggtaaaactc
    34021 ggcatcctga aaggtgatgt tgatgaactg atcgctcaga acgcccatcg tcctttcttt
    34081 atgcatggcc ttagccactg gttaggactg gatgtccatg acgttggcgt ttatggccag
    34141 gatcgctcgc gcattctgga accgggcatg gtactgaccg tagagccagg gctgtatatt
    34201 gcgccggatg cagaagtgcc agaacaatat cgcggtatcg gcattcgtat tgaagacgac
    34261 attgtgatta ccgaaaccgg taacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa
    34321 gaaatcgaag cgttgatggc agctgcgaga aagtaatgag cgtaatcatc gtcggtggcg
    34381 gcatggcggg cgcgacgctg gcgctggcta tttcccggtt aagtcacgga gcgctgccgg
    34441 tacatttgat tgaagcgacc gcgccagagt cacatgctca tccgggcttt gatggacgag
    34501 ctattgctct agcggcgggg acctgtcaac aactggcgcg catcggcgtc tggcaatctc
    34561 tggctgattg cgcaaccgcc atcactaccg tgcatgtcag cgatcgtggt catgccggat
    34621 tcgtcaccct cgccgcagaa gattaccaac tggcggcgct ggggcaggtt gtcgaattgc
    34681 acaatgtcgg gcaacggctg tttgcattgc tgcgcaaagc acctggcgta acgctgcatt
    34741 gcccggatcg cgtggctaac gttgcccgta ctcagagtca tgttgaagtg acgctggagt
    34801 gtggcgagac gctgacgggc cgcgtgctgg tagccgctga tggcacccat tcagcgttag
    34861 ctactgtgtg cggcgttgac tggcagcagg agccttacga acaactggct gtgattgcca
    34921 acgttgcgac ttccgttgcg catgaaggac gcgcttttga acgctttacg caacatggcc
    34981 cgctggcgat gttgccgatg tctgatggac gctgttcgct ggtctggtgt catccactgg
    35041 aacggcgcga agaggtgctg tcgtggagcg acgagaagtt ttgccgtgaa ctccagtcgg
    35101 cctttggctg gcgacttggg aaaattaccc acgctggtaa acgcagtgct tatccgctgg
    35161 cgttaaccca cgccgccaga tctattaccc atcgtaccgt gctggtgggc aatgcggcgc
    35221 aaaccctgca ccccatcgct gggcaaggat ttaatctcgg tatgcgtgat gtgatgagcc
    35281 ttgcggaaac cctgactcag gcgcaggagc gcggagaaga catgggtgat tacggcgtat
    35341 tgcgccgtta tcagcagcgt cgacagagcg atcgagaagc aaccatcggc gtcacggaca
    35401 gccttgtaca tctttttgcc aaccgttggg cgccgctggt tgtcgggcgc aacattgggc
    35461 tgatgacgat ggaattattc accccggcac gcgatgtgct ggcgcagcgc accctcggtt
    35521 gggtggcgcg ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga tgtagccatt
    35581 gttggcggcg gcatggtggg gctggcggtt gcctgcggct tacagggtag cggcttacgc
    35641 gttgccgtac tggagcagcg cgtaccggaa cctctggcgg cggatgcacc atcacaactg
    35701 cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca cccgtcttgg cgtctggcag
    35761 gacattctct ctcgccgagc cagctgttat cacggtatgg aagtgtggga caaagacagc
    35821 tttggtcaca tttcgtttga cgatcaaagc atgggctata gccatcttgg gcatatcgtt
    35881 gaaaactcag tgattcacta cgcgctgtgg aacaaagcgc agcaggcgtc agatatcact
    35941 ctgttggccc ccgcagaatt acagcaggtc gcctggggag aaaacgaaac cttcctgacg
    36001 ctgaaagatg gcagtatgtt aacggcgcgt ctggtgattg gcgcggacgg cgctaattcc
    36061 tggttgcgca acaaagccga tattccgctg actttctggg attatcagca tcacgcgctg
    36121 gtagcgacca tacgcacgga agaaccgcat gatgcggtgg cgcggcaggt tttccatggc
    36181 gaaggcattc tggccttttt accgcttagc gatccgcatc tttgctcgat tgtctggtca
    36241 ctgtcgccag aggaagcgca gcggatgcag caggcagggg aagacgaatt taatcgcgcg
    36301 ttaaatatcg cttttgataa tcgcctgggc ttatgcaagg ttgagagcga gcgtcaggtg
    36361 ttcccgctga cggggcgtta tgcgcgccag tttgccgcac accgtctggc gctggtgggc
    36421 gacgccgcgc ataccattca cccgctggcg gggcagggcg ttaatctcgg ctttatggac
    36481 gccgcagagc tgattgctga actgaaacgg ttgcatcgtc agggtaaaga catcgggcag
    36541 tacatttacc tgcgccgcta tgaacgtagc cgcaagcaca gcgcggcgct gatgctggct
    36601 ggtatgcagg gattccgcga tctgttttcc ggtaccaatc cggcgaaaaa actgctgcgt
    36661 gatattggtt tgaaactggc cgacacgctt cctggcgtta agccacaact tatccgtcag
    36721 gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa aatttctctt ctgctgttta
    36781 tttgatacgc atcacacttt catctcccgg ttttttcgcc gggagatttt cctcatttga
    36841 aataaactaa tttcacctcc gttttcgcat tatattttct aatgccatga ttttttgatt
    36901 tagtgttttt tgacattttt ttagctctta atattgtctt attcaaattg actttctcat
    36961 cacatcatct ttgtatagaa aatggtgtgt tttttggttt tttattctgt cgcgattttt
    37021 gcatttttta accataagct aatgtgatga tcaattttac cttatggtta acagtctgtt
    37081 tcggtggtaa gttcaggcaa aagagaacga ttgcgttggg gaccgggagt ggctccgatg
    37141 ctgggttttg tggtgataat ttcaccatga aaaagttgtc agccccgctt attcaatgag
    37201 gacaagatgg cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg
    37261 gtggatttcc acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat
    37321 gcggtacgta ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt cgatcttcgc
    37381 ggcagccgca cccgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc
    37441 aaaagcggca aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgat
    37501 ctcatcgtct actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc
    37561 gaaaaagacc tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt
    37621 cgcgatgacc tttccatgat tgccgtgcaa gggccgaatg cgcaggcaaa agctgccaca
    37681 ctgtttaatg acgcccagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag
    37741 gcgggggatc tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg
    37801 ctgcccaatg aaaaagcggc ggatttctgg cgcgcgctgg tggaagcggg cgttaagcca
    37861 tgcggcctgg gcgcgcgtga cacgctgcgt ctggaagctg gcatgaattt gtatagtcag
    37921 gagatggacg aaaccatctc tcctttagcc gccaacatgg gctggaccat cgcctgggaa
    37981 ccggcagatc gtgactttat cggtcgtgaa gctctggaag cgcagcgtga acatggcaca
    38041 gaaaaactgg ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta
    38101 cgttttaccg atgcgcaggg caaccagcat gaaggcatta tcaccagcgg gactttctcc
    38161 ccgacgctgg gttacagcat tgcgctggcg cgcgtgccgg aaggtattgg tgaaacagcg
    38221 attgtgcaaa ttcgcaaccg tgaaatgccg gttaaagtga cgaaacctgt ttttgtgcgt
    38281 aacggcaaag ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag
    38341 cagaactgaa atacagcaaa gaacacgaat ggcttcgtaa agaagccgac ggcacttata
    38401 ccgttggcat caccgaacac gctcaggagt tgttgggcga tatggtgttt gttgacctgc
    38461 cggaagtggg cgcgacggtt agcgcgggcg atgactgcgc ggttgctgaa tcggtaaaag
    38521 cggcgtcaga catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgtactga
    38581 gcgattcccc ggaactggtg aacagcgaac cgtatgcagg cggctggatt ttcaaaatca
    38641 aagccagcga tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt
    38701 tagaagacga gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaaattt
    38761 acgcctcacc ctcactctct tcgtaaggag agaggttcac aattcactgc acgtttcagg
    38821 aaccatcgct catgacacag acgttaagcc agcttgaaaa tagcggcgct tttattgaac
    38881 gccatatcgg accggacgcc gcgcaacagc aagaaatgct gaatgccgtt ggcgcacaat
    38941 cgttaaacgc gctgaccggc cagattgtac cgaaagatat tcagcttgcg accccgccgc
    39001 aggttggcgc accggcgacc gaatacgccg cgctggcgga actcaaggct attgccagtc
    39061 gcaataaacg cttcacgtct tacatcggca tgggttacac cgccgtgcag ttaccgccgg
    39121 ttatcctgcg taacatgctg gaaaatccgg gctggtatac cgcgtatact ccgtatcaac
    39181 ctgaagtctc gcagggccgc cttgaggcac tgctcaattt ccagcaggta acgctggatt
    39241 taaccgggct ggatatggcc tctgcttctc ttcttgacga agccaccgct gccgccgaag
    39301 caatggcgat ggcgaaacgc gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt
    39361 ccgatgtgca tccgcaaacg ctggatgtgg tccgtactcg tgccgaaacc tttggttttg
    39421 aagtgattgt cgatgatgcg caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt
    39481 tacagcaggt tggcactacc ggtgaaattc acgactacac tgcgcttatc agcgaactga
    39541 aatcacgcaa aattgtggtc agcgttgccg ctgatattat ggcgctggtg ctgttaactg
    39601 cgccgggtaa acagggcgcg gatattgttt ttggttcggc gcaacgcttt ggcgtaccga
    39661 tgggttacgg tggcccacac gcggcattct ttgcggcgaa agacgaatac aaacgctcaa
    39721 tgccaggccg tattatcggt gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg
    39781 cgatgcagac tcgcgagcaa catattcgtc gtgagaaagc gaactccaac atttgtactt
    39841 cccaggtgct gctggcaaac atcgccagcc tgtatgccgt ttatcacggc ccgattggcc
    39901 tgaaacgtat cgctaaccgc attcaccgtc tgaccgatat cctggcggca ggcctgcaac
    39961 aaaaaggtct gaagctgcgc catgcgcact atttcgacac tctgtgtgtg gaagtggccg
    40021 acaaggcggg cgtactggcg cgtgccgaag cggctgaaat caacctgcgt agcgatatcc
    40081 tgaacgcggt tgggatcacc cttgatgaaa ccaccacgcg cgaaaacgtg atgcagcttt
    40141 tcagcgtact actgggggat aaccacgggc tggagatcga cacgctggac aaagacgtgg
    40201 ctcacgacag ccgctctatt caacctgcga tgctgagtga tgacgaaatc ctcacccatc
    40261 cggtgtttaa tcgctaccac agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc
    40321 gtaaagatct ggcgctgaat caggcgatga tcccgctggg ttcctgcacc atgaaactga
    40381 acgccgccgc cgagatgatc ccgatcacct ggccggaatt tgccgaactg catccgttct
    40441 gcccgccgga gcaggccgaa ggttatcagc agatgattgc gcagctggct gactggctgg
    40501 tgaaactgac cggttacgac gccgtttgta tgcagccgaa ctctggcgca cagggcgaat
    40561 acgcgggcct gttggcgatt cgtcattatc atgaaagccg caacgaaggg catcgcgata
    40621 tctgcctgat cccggcttct gcgcacggca ctaaccctgc ttctgcacat atggcaggaa
    40681 tgcaggtggt ggttgtggca tgtgataaaa acggcaacat cgatctggct gatctgcgcg
    40741 cgaaagcgga acaggcgggc gataacctct cctgtatcat ggtgacctat ccttccaccc
    40801 acggcgtgta tgaagaaacg atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc
    40861 aggtttacct tgatggcgcg aacatgaacg cccaggttgg catcacctcg ccgggcttta
    40921 ttggtgcgga cgtttcacac cttaacctac ataaaacttt ctgcattccg cacggcggtg
    40981 gtgggccggg tatgggaccg atcggtgtga aagcgcatct ggctccgttt gtaccgggtc
    41041 atagcgtggt gcaaatcgaa ggcatgttaa cccgtcaggg cgcggtttct gcggcaccgt
    41101 tcggtagcgc ctctatcctg ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag
    41161 ggctgaaaaa agcaagccag gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc
    41221 aggatgcctt cccggtgctg tataccggtc gcgacggtcg cgtggcgcac gaatgtattc
    41281 tcgatattcg cccgctgaaa gaagaaaccg gcatcagcga gctggatatt gccaagcgcc
    41341 tgatcgacta cggtttccac gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg
    41401 ttgaaccgac tgaatctgaa agcaaagtgg aactggatcg ctttatcgac gcgatgctgg
    41461 ctatccgcgc agaaattgac caggtgaaag ccggtgtctg gccgctggaa gataacccgc
    41521 tggtgaacgc gccgcacatt cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc
    41581 gtgaagttgc ggtattcccg gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc
    41641 tggatgatgt ttacggtgac cgtaacctgt tctgttcctg cgtaccgatt agcgaatacc
    41701 agtaatttac tggttcgact atcttctaaa ggcgcttcgg cgccttttta gcttgttaat
    41761 aaagtgcgct ttgtgtctcg gatgaagcgt aaatataaaa tcctggagtt caatatatga
    41821 caggagcgcc aggcctgatg agcgtatcgc atcaggcaca tcggctaatt acagattctt
    41881 gccgttgctg gcaatcacct ctttgtacca gttaaagctc ttcttacgtg aacgcgacat
    41941 atcgccagta ccgtcgtcat gtttattcac atagataaag ccgtagcgtt tgctgtactg
    42001 cccggtggtg aacgacacgc agtcgatgca gccccacggc gtgtagccca tcagatccac
    42061 gccatcgtaa gtcaccgctt ttttcatctc ttcgatatgg gcgcgcaggt agtcaatgcg
    42121 gtagtcgtcg ttgatgctgc catcttcttc cactttgtcg taagcgccaa aaccgttttc
    42181 gacaataaac agcggcttct ggtaacgctc atacagttcg caaagcgcgt aacgcagacc
    42241 cacagggtca atctgccagc cccagtcaga cgctttaaca tacgggtttg gtacgctgcc
    42301 ttcaaaacca gagatagcat cgccggtgcc gccttcggcc ttcactgcgt tggtcatgta
    42361 atagctgaaa ccaagataat cgcaggtgcc ttcgcgcagt atatcgagat cgccatcttc
    42421 cattttgatg ttaaatccgc gacgctccca ctcgttcaac acataggacg ggtaatagcc
    42481 gcgtagctgc acatcggtaa agacgtagcg ttcgcgcatc gactcctgag cgaacatcac
    42541 atcgtccggg ttacaggagt aaggatagag cggcaccatc gccagcatac agccgacttt
    42601 catctccggg ttaatgcgac gcgcagcttt caccgccagg gcgctggcga caaactggtg
    42661 atgcagcacc tgatacatcg tctcttccgg gttctcatgc tcggtataaa ccacgccgga
    42721 gcagcagtaa ccgaacagcg gtgcacgcca gttacgctgg ttgttaattt cgttgaaggt
    42781 catccagtat ttgactttat gcttatagcg ttcaaatacc acttccgcga aacggacaaa
    42841 gaaatcaacc actttacggt tggtccagct accgtattgc tgcaccagat gcagcggcat
    42901 ttcaaagtgg gagagggtga tcactggttc gatgttgtat ttcagcaatt catcgaacag
    42961 agagtcgtaa aacttcagcc cttcttcgtt tggctgagct tcatcgcctt ttggaaaaat
    43021 gcgcgtccag gcgatggatg tacgaaaaca tttgaagccc atttcggcaa atagcttgat
    43081 gtcttccttg tagtgaccat aaaaatcaac ggcttcatgg tttggatagt attttcctgg
    43141 caagacttct ttggtgattt cgcgcggcac gccgtgtgcg ccaccggtca gaacgtcaca
    43201 aatgctcggc cctttgccgc ctttgttcca gccgccttcg acctgatgag cggcaactgc
    43261 gccgccccat aagaaatctt tcggtaaggt gagttttttc actatcatag gctccagatg
    43321 agtcaattct tacaggaaat agtaccatgc agccaaacga catcactttt tttcaacgtt
    43381 tccaggatga cattctggct gggcgtaaaa ccatcaccat ccgcgacgag tctgaatcgc
    43441 acttcaaaac gggtgatgtg cttcgtgtcg ggcgttttga agatgacagt tatttttgca
    43501 cgattgaagt caccgcaacc tcaaccgtaa cgctggatac gctgacggaa aaacatgcag
    43561 agcaggaaaa tatgaccctg actgaactga aaaaggtcat tgccgacatc tatcccggtc
    43621 agacacagtt ttatgtgatt gaatttaaat gtctttaaat tgaactcacg cctgttagtt
    43681 gaaatttcgc atttaatttc atgatttatc ggagattaat cccaaatcag aatttatttt
    43741 agctaacagg tgttcactgg aactattctc agttacgctg gagaggtata catgtgcgtg
    43801 tacgtttctc cggagtaagt tatggttcag aagcccctca ttaagcaggg atattcgctg
    43861 gcagaggaaa ttgccaacag cgtcagtcac ggcattgggt tggtgtttgg tatcgttggg
    43921 ctggtgttgc tactggttca ggcggtggat ctcaatgcca gcgccacagc gataaccagc
    43981 tacagcctct atggcggcag tatgatcctg ctgttcctcg cttcgacgct ctatcacgcc
    44041 attcctcatc aacgggcaaa aatgtggctg aagaaatttg accattgcgc tatttacctg
    44101 ttgattgctg gaacctacac gccgtttttg ctggtggggc tggattctcc gttagcgcgc
    44161 gggttgatga ttgttatctg gagcctggca ttgctgggga ttctgttcaa actgaccatc
    44221 gcgcaccgat tcaaaatttt atctctggtg acttatctgg cgatgggctg gctgtcgctg
    44281 gtggtaattt atgaaatggc agttaagctc gcggcgggca gcgttacctt actggcggta
    44341 ggcggcgtgg tttattcgct cggggtgatt ttctacgtct gcaaacgcat tccatacaac
    44401 catgccatct ggcacggctt cgtgctcggc ggtagtgtgt gccactttct ggcgatctat
    44461 ttgtatattg ggcaggcgta atgggtatgc ctttgtaggc ctgataagac gcgccagcgt
    44521 cgcatcaggc gataaatgcc tgatgcgaca cagagattta ctcttcgagc gaatacggca
    44581 gcggctcgat atgcaatgta ttcgcatcgt cacgcacgcg gaagatgctg tccggttcca
    44641 tatcgttatt catgacgacc tgtaccacga cctgaccatc ttccagtttt accgcagcca
    44701 gcaccgtacc ggtgcgacgc cagttctcgc ccattttcag ctctaaatct tcaccagctt
    44761 ccggcagtcg gctggcgcta cctgccagca accagagcgc acgtttattg gcaccacgga
    44821 attttgctcg cgccaccatc tcttgtccgg tataacagcc tttcttaaag ctgataccgc
    44881 ccagcgcctg gagattggtc gcctgtggga taaactgccc gctgttcgcg gcatcaatca
    44941 ccgggaaacc cgcttgaatg tttaatgcca gccactgttg gctattgttc agttccgctt
    45001 caccgcgcag tttatcggtc agcatattgg cagtagcttc atcggttacg atcaggaaac
    45061 gttctgccgg gtgttcaaac catagcaaag tggtcgcgcc ttctttaact acctgttttt
    45121 ctctcgaagg tagttcacta aagagattgg ccagtgcggc gcgcgcctga aaaccggcaa
    45181 caccaagcag cacacgctcg tcgtctggcg cgatggtcac tttagagaat accgcatatt
    45241 ttttcagttc agtcagctgc ggttcacgca cgctgcgccg ttcaatccac gcaaagccat
    45301 cgccgtcgcg gaacagacgt aaattgctcc acattttacc tttggcgtcg caatgggcgg
    45361 cgagcaggtg ctgattttct gtcatctggc tgacatctgc tgtcacctgg ccctgcatat
    45421 atttttcgct gtccgcgcca gtaatggtgg caagcgccca gtcatcaagc gtcatcagtg
    45481 tcagtggcaa acgggcagaa gccgtaggct gacgtggagg aaaaggtgta aaagccataa
    45541 tagagtcctg agttgcttaa cggcgtatta ataactaatg gtaaaagagc tatgaggcaa
    45601 tgcaagtgct ttcactacgc gatctgtgcc ttctgcgagg cgacttccaa aaaaatgaag
    45661 cccttgaatt gttgtgaatt aatttcgcga tcgcgattgt gtttactgat attcccgtaa
    45721 aagaagtcga aaaacacgtt acaatagcgg ggtactggat tttcgtgaga caggaagaag
    45781 atggacatta acaacaaagc ccgcattcat tgggcatgcc gccgtggtat gcgcgaactc
    45841 gatatttcaa tcatgccgtt tttcgaacat gagtacgaca gcttaagcga tgacgaaaaa
    45901 cgcatcttta ttcgtctgct ggaatgtgac gatccggacc tgttcaactg gctaatgaat
    45961 cacggtaaac cggccgatgc agaactggaa atgatggtcc gactcatcca gacacggaac
    46021 cgggaacgtg gtcctgtggc aatctgattt gcgcgtctcc tggcgcgcac agtggctttc
    46081 cttgctgatt catgggctgg ttgccgctgt tattttactc atgccctggc cgctcagtta
    46141 caccccgtta tggatggtgt tactttcgct ggtggtgttt gattgcgttc gcagccagcg
    46201 gcgtattaat gctcgccagg gggaaattcg cttgttgatg gacgggcgtt tgcgttggca
    46261 agggcaggag tggagcatcg tcaaagcacc gtggatgatt aagagcggca tgatgctgcg
    46321 tttacgttct gatggcggta aacggcaaca tttatggctg gcagccgaca gcatggacga
    46381 agccgaatgg cgggatttac ggcggatttt gttgcaacaa gagacgcaaa gataaagatg
    46441 acggcaaagc atatgcgcta agccgtcagt gagatcaggc gtaatgctct gccatttcgt
    46501 tgaggatttg ctcgcaccag ctctgaatac gctcgtcgct aaggtcatac tggttagttt
    46561 catccagcgc cagacccacg aacagttgcc cgtcagcaat caccggtttc gggctggtaa
    46621 attcatatcc ttccgttggc cagtagccga cgaacttcac gcctttggta gaaagtttgt
    46681 catgcagcat ccctagcgca tcgaggaacc attcaccgta cccgagttga tcgccaagcc
    46741 catacagcgc aacaatttta ccttcgaggt tcaggtcgtc gagctgatcc cagacggctt
    46801 cccagtcttc ctggatttcg ccaaaatccc aggtcgggat acccagaatg agcacatcgt
    46861 actgctccat taatttcggg gagtcgtcct tgaggttatg taaggtcacc agttctgggc
    46921 cgataatatc gcggattttt tctgccgcca tttcggtgta acaggtgctg gaaccgtaaa
    46981 aaagacccat attcataacg taagcttctc gattctgctg tggcttttgc ggctagtgta
    47041 tcagaatcgc tttagtcacg gcacaataag gcataatgga tcaaatgagt gaccataagg
    47101 ggcgcaagtg aaacaggatc tggcacgcat cgagcagttt cttgatgctc tgtggctgga
    47161 aaaaaatctg gctgaaaata cgttgaacgc ttaccgtcgc gatctgtcaa tgatggtgga
    47221 gtggttgcat caccgcgggt tgacgctggc gacggcgcaa agtgacgatt tacaggcatt
    47281 actggcagaa cggctggagg gcgggtataa agccaccagc tcagcgcgtt tgctgagtgc
    47341 agtgcgccga ttgttccagt atctttatcg cgaaaagttt cgtgaagacg atcccagtgc
    47401 gcatctcgct tcaccgaaat tgccccagcg tttgccaaaa gatttaagcg aagcgcaggt
    47461 cgaacgttta ttacaggcac cattaattga tcagccactg gagctacgcg ataaagccat
    47521 gcttgaagtg ttgtatgcta ccgggctgcg tgtctctgaa ctggtcggac tgacaatgag
    47581 tgatatcagc ctgcgtcagg gcgtagtgcg ggtcattggt aaaggcaaca aagagcgctt
    47641 ggtaccgtta ggtgaagagg cggtttactg gctggaaacc tatctggaac atgggcgtcc
    47701 gtggctgctg aatggtgtgt caattgacgt gttgtttccc agccagcgtg cgcagcagat
    47761 gacgcgacag accttctggc accgtattaa acattatgcc gtgctggcgg gtatcgacag
    47821 cgaaaagctg tcaccgcatg tgttgcgtca cgcttttgcc actcatttat taaatcatgg
    47881 tgcggattta cgcgtggtgc agatgctact gggccacagc gatctttcca ccacgcaaat
    47941 ttatacgcat gtcgctactg agcgtctgcg gcaacttcat caacagcatc acccgcgggc
    48001 gtgatgtctg aaaagaacgg gaagatttat gaagaaaggt tttatgttgt ttactttgtt
    48061 agcggcgttt tcaggctttg ctcaggctga tgacgcggca attcaacaaa cgttagccaa
    48121 aatgggcatc aaaagcagcg atattcagcc cgcgcccgta gctggcatga agacagttct
    48181 gactaacagc ggtgtgttgt acatcaccga tgatggtaaa catatcattc aggggccaat
    48241 gtatgacgtt agcggcacgg ctccggtcaa tgtcaccaat aagatgctgt taaagcagtt
    48301 gaatgcgctt gaaaaagaga tgatcgttta taaagcgccg caggaaaaac acgtcatcac
    48361 cgtgtttact gatattacct gtggttactg ccacaaactg catgagcaaa tggcagacta
    48421 caacgcgctg gggatcaccg tgcgttatct tgctttcccg cgccaggggc tggatagcga
    48481 tgctgagaaa gaaatgaaag ctatctggtg tgcgaaagac aaaaacaaag cgtttgatga
    48541 tgtgatggca ggtaaaagcg tcgcaccagc cagttgcgac gtggatattg ccgaccatta
    48601 cgcacttggc gtccagcttg gcgttagcgg tactccggca gttgtgctga gcaatggcac
    48661 acttgttccg ggttaccagc cgccgaaaga catgaaagaa ttcctcgacg aacaccaaaa
    48721 aatgaccagc ggtaaataat tcgcgtgaaa caacagatac aacttcgtcg ccgtgaagtc
    48781 gatgaaacgg cagacttgcc cgctgaattg cctcccttgc tgcgccgttt atacgccagc
    48841 cgaggagtac gcagtgcgca agaactggaa cgcagtgtta aaggtatgtt gccctggcag
    48901 caactgagcg gcgtcgaaaa ggccgttgag atcctttaca acgcttttcg cgaaggaacg
    48961 cggattattg tggtcggtga tttcgacgcc gacggcgcca ccagcacggc tctaagcgtg
    49021 ctggcgatgc gctcgcttgg ttgcagcaat atcgactacc tggtaccaaa ccgtttcgaa
    49081 gacggttacg gcttaagccc ggaagtggtc gatcaggccc atgcccgtgg cgcgcagtta
    49141 attgtcacgg tggataacgg tatttcctcc catgcggggg ttgagcacgc tcgctcgttg
    49201 ggcatcccgg ttattgttac cgatcaccat ttgccaggcg acacattacc cgcagcggaa
    49261 gcgatcatta accctaactt gcgcgactgt aatttcccgt cgaaatcact ggcaggcgtg
    49321 ggtgtggcgt tttatctgat gctggcgctg cgtacctttt tgcgcgatca gggctggttt
    49381 gatgagcgta acatcgcaat tcctaacctg gcagaactgc tggatctggt cgcgctgggg
    49441 acagtggcgg acgtcgtgcc gctggacgct aataatcgca ttctgacctg gcaggggatg
    49501 agtcgcatcc gagccggaaa gtgccgtccg gggattaaag cgctgcttga agtggcaaac
    49561 cgtgatgcac aaaaactcgc cgccagcgat ttaggttttg cgctggggcc acgtctcaat
    49621 gctgccggac gactggacga tatgtccgtc ggtgtggcgc tcttgctgtg cgacaacatc
    49681 ggcgaagcgc gcgtgctggc aaatgaactc gatgcgctaa accagacgcg aaaagagatc
    49741 gaacaaggaa tgcaggttga agccctgacc ctgtgcgaga aactggagcg cagccgcgac
    49801 acgctacccg gcgggctggc aatgtatcac cccgaatggc atcagggcgt tgtcggtatt
    49861 ctggcttcgc gcatcaaaga gcgttttcat cgtccggtta tcgcctttgc gccagcaggt
    49921 gacggtacgc tgaaaggttc cggtcgctcc attcaggggc tgcatatgcg tgatgcactg
    49981 gagcgattag acacactcta ccctggcatg atgctgaagt ttggcggtca tgcgatggcg
    50041 gcgggtttgt cgctggaaga ggataaattc gaactctttc aacaacggtt tggcgaactg
    50101 gttacagagt ggctggaccc ttcgctattg caaggcgaag tggtgtcaga cggtccgtta
    50161 agcccggccg aaatgaccat ggaagtggcg cagctgctgc gcgatgctgg cccgtggggg
    50221 cagatgttcc cggagccgct gtttgacggt catttccgtc tgctgcaaca gcggctggtg
    50281 ggcgaacgtc atttgaaagt catggtcgaa ccggtcggcg gcggtccgct gctggatggt
    50341 attgctttta atgtcgatac cgccctctgg ccggataacg gcgtgcgcga agtgcaactg
    50401 gcttacaagc tcgatatcaa cgagtttcgc ggcaaccgca gcctgcaaat tatcatcgac
    50461 aatatctggc caatttagcg tcatcttctc tataaaaaag agcgtggatt gggtacaatc
    50521 ccgctcttat caccgcattt tgactagctc aataaaagaa atcagaccat gtttgaaatt
    50581 aatccggtaa ataatcgcat tcaggacctc acggaacgct ccgacgttct tagggggtat
    50641 ctttgactac gacgccaaga aagagcgtct ggaagaagta aacgccgagc tggaacagcc
    50701 ggatgtctgg aacgaacccg aacgcgcaca ggcgctgggt aaagagcgtt cctccctcga
    50761 agccgttgtc gacaccctcg accaaatgaa acaggggctg gaagatgttt ctggtctgct
    50821 ggaactggct gtagaagctg acgacgaaga aacctttaac gaagccgttg ctgaactcga
    50881 cgccctggaa gaaaaactgg cgcagcttga gttccgccgt atgttctctg gcgaatatga
    50941 cagcgccgac tgctacctcg atattcaggc ggggtctggc ggtacggaag cacaggactg
    51001 ggcgagcatg cttgagcgta tgtatctgcg ctgggcagaa tcgcgtggtt tcaaaactga
    51061 aatcatcgaa gagtcggaag gtgaagtggc gggtattaaa tccgtgacta tcaaaatctc
    51121 cggcgattac gcttacggct ggctgcgtac agaaaccggc gttcaccgcc tggtgcgtaa
    51181 aagcccgttt gactccggcg gtcgtcgcca cacgtcgttc agctccgcgt ttgtttatcc
    51241 ggaagttgat gatgatattg atatcgaaat caacccggcg gatctgcgca ttgacgttta
    51301 tcgcgcgtcc ggcgcgggcg gtcagcacgt taaccgtacc gaatctgcgg tgcgtattac
    51361 ccacatcccg accgggatcg tgacccagtg ccagaacgac cgttcccagc acaagaacaa
    51421 agaccaggcc atgaagcaga tgaaagcgaa gctttatgaa ctggagatgc agaagaaaaa
    51481 tgccgagaaa caggcgatgg aagataacaa atccgacatc ggctggggca gccagattcg
    51541 ttcttatgtc cttgatgact cccgcattaa agatctgcgc accggggtag aaacccgcaa
    51601 cacgcaggcc gtgctggacg gcagcctgga tcaatttatc gaagcaagtt tgaaagcagg
    51661 gttatgagga accaacatgt ctgaacaaca cgcacagggc gctgacgcgg tagtcgatct
    51721 taacaatgaa ctgaaaacgc gtcgtgagaa gctggcgaac ctgcgcgagc aggggattgc
    51781 cttcccgaac gatttccgtc gcgatcatac ctctgaccaa ttgcacgcag aattcgacgg
    51841 caaagagaac gaagaactgg aagcgctgaa catcgaagtc gccgttgctg gccgcatgat
    51901 gacccgtcgt attatgggta aagcgtcttt cgttaccctg caggacgttg gcggtcgcat
    51961 tcagctgtac gttgcccgtg acgatctgcc ggaaggcgtt tacaacgagc agttcaaaaa
    52021 atgggacctc ggcgacatcc tcggcgcgaa aggtaaactg ttcaaaacca aaaccggcga
    52081 actgtctatc cactgtaccg agctgcgtct actgaccaaa gcactgcgtc cgctgccgga
    52141 taaattccac ggcttgcagg atcaggaagc gcgctatcgt cagcgttatc tggatctcat
    52201 ctctaacgat gaatcccgta acacctttaa agtgcgctcg cagatcctct ctggtattcg
    52261 ccagttcatg gtgaaccgcg gctttatgga agttgaaacg ccgatgatgc aggtgatccc
    52321 aggcggtgcc gctgcgcgtc cgtttatcac ccaccataac gcgctggatc tcgacatgta
    52381 cctgcgtatc gcgccggaac tgtacctcaa gcgtctggtg gtcggtggct tcgagcgtgt
    52441 attcgaaatc aaccgtaact tccgtaacga aggtatctcc gtacgtcata acccagagtt
    52501 caccatgatg gaactctaca tggcttacgc agattacaaa gatctgatcg agctgaccga
    52561 atcgctgttc cgtactctgg cacaggatat tctcggtaag acggaagtga cctacggcga
    52621 cgtgacgctg gacttcggta aaccgttcga aaaactgacc atgcgtgaag cgatcaagaa
    52681 atatcgcccg gaaaccgaca tggcggatct ggacaacttc gactctgcga aagcgattgc
    52741 tgaatctatc ggcatccacg ttgagaagag ctgggggctg ggccgtatcg ttaccgagat
    52801 cttcgaagaa gtggcagaag cacatctgat tcagccgacc ttcattactg aatatccggc
    52861 agaagtttct ccgctagcgc gtcgtaacga cgttaacccg gaaatcacag accgctttga
    52921 gttcttcatt ggtggtcgtg aaatcggtaa cggctttagc gagctgaatg acgcggaaga
    52981 tcaggcgcag cgcttcctgg atcaggttgc cgcgaaagac gcaggtgacg acgaagcgat
    53041 gttctacgac gaagattacg ttaccgcact ggaacatggc ttaccgccga cagcaggtct
    53101 gggaattggt atcgaccgta tggtaatgct gttcactaac agccatacca tccgcgacgt
    53161 tattctgttc ccggcgatgc gtccggtgaa ataagcatta cgttatgctc acaaccccgg
    53221 caaatgtcgg ggttttttat ttaagctggg taaatgcaga taatcgtatt ctggcttcgc
    53281 gatttgtcgc ctgcatcacc atccacggac tgaacgccca cggcgtggca tcaataccgc
    53341 gtaatacatc agctaaatca caccattgat aatccatcac ttcatcatcg ttgatctgta
    53401 acgcactggt ggtgcgtgcg gcaaataccg gacacacttc attttccaca atgccattcg
    53461 gatcggtggc gcgatagcga aagtcaggat agatagattc aggaggcgta atttccacgc
    53521 caagctcata acggcaacgg cggatcaccg cgtcttcgtt gctttctccc agttgtgggt
    53581 gcccacaaac cgagttagtc cacacgccag gccatgcttt tttgctcagt gcgcggcggg
    53641 taactaataa ttgtcctttg gcattaaaca gccaactgga gaacgcgaga tgtaagcggg
    53701 tgtctgccgt gtgtgcggca tacttttcca gcgtacccgt gggaactccc tgtgcattca
    53761 ataaaatgac gtgttccgtt tgcataattt ctcacatgta attctgatca ctcatcatta
    53821 tagataattg aagagtgaaa tgattaacca agtgagcgtt tatcgacaac cgcccgtttt
    53881 gagcggatgt cgacaggtaa aaaccattta aaaaagtagc atcatatatt tgaagcgact
    53941 ttcaatggcc attgcaacac agtcagaaat gacaggaata gagagagtaa aaagcaacag
    54001 cagactgaat atagcgcgca ttaaaaaatt catattattc tccatgctca tttttcttca
    54061 gcttgcgcat cagaagccac atacgtactg tcctgacttt taaatcttta tcagagacgg
    54121 attccttcac caggtcagca tgatgctggt agccattaaa gaagacattc agcacaacgg
    54181 cgctaagggt tgcgagcata atgccgctat gtagcagcgg ttgcagtacg gccggtaact
    54241 tagaaaagaa atcgtgagag agcgtcggag tcatgccaac gccgagactg atcgccacaa
    54301 tatagaggtt gtaacggttg gtggtgtagt tacagcgcga cagaattcga atccctgtcg
    54361 ccagtaccat gccgaacatc accagcccag cgccgcccag cacaaattgc ggaatggagg
    54421 ctaccagcac cgccattttt ggcaccatgc caaacaggat taatataatt cccgaagaaa
    54481 tacacaccca gcggctatga acgcgcgtca cgctaaccag gccaacgttt tgagaaaatg
    54541 acgtgtgggg gaagctgtta aacgtgccac caatcattgt ccctacgcca tcgacacgca
    54601 gcccgcgaat aatatcgtgc gaagagagtt tacgaccgac tatttcaccc agtgccagga
    54661 acatccccat cgactcgata aacacgatga ttaacacggc agtcatggtc agaatggaaa
    54721 cgggatcgaa aatcggcata ccaaacgaca tcggggtgac aatcgcaaac cacgaggcat
    54781 catgtagccc ggataaattg acttcattca tcatccacga aagtaaaaag ccaaatacaa
    54841 tccccagtaa tacggcgacg ttggacataa atcctttcgc atagcgagta atgagcaaga
    54901 taaaaattaa gacggcaaag gagataccta aataaacggg attaccatat tgcggattcc
    54961 ctttacctcc ggcagcccag tcaataccca cctgaatgat gctaagcccg atagaagtaa
    55021 taaccacacc ggtaaccagt ggcgggaata aaggcatcaa gcgaccgata agtggcgcta
    55081 ataatgtggt gataaaaccc gcggcgatag tggcaccaaa tatccccagc aggccgatat
    55141 ccgggttcat cccaatggct atcattggtg ttacagcagc aaaggtcacc gacataatca
    55201 ccggcaggcg gatccccata aagcggccga taccgataca ttgcaataat gtgacgatcc
    55261 cgcagcaaaa gagatccgag ctaatgagca tcgcaatagc atcttttgag aggcccagtc
    55321 ggtcaccaat cataagagga acagcgactg cacctgcgta catgacaagg acgtgttgca
    55381 gaccgagtat gatcagcttt cctggtgata atatgcggtc aacctcatca gttgggcggt
    55441 cttgcccaga agatgaggga agttgggaat ctatggcgct catcagtgtt tctcctttcg
    55501 cccgccagag taaaaagcaa ccgtttactt taatgaaact gtctaaacac atgctcgtgg
    55561 agtgccagtt tttgcgagga aggggagtta gagacaggaa ttgcggaggc aatcacataa
    55621 tcgtggttaa accacgaaaa tgagaagaga acctgacaat ggaattatct gtaattttct
    55681 catattgata tttcattaat cattctggca tgtcgcactc tcgcatttaa tcgtttttat
    55741 ctggatagcg ctcttttgat cggcaatgtt gaaataatga aagcggcata gtttgagggg
    55801 caataatttg aactcagtcg caaagttata attaaaacgg tcatgctacc taatatatgc
    55861 ccgtttattg catcgcgaaa tttgagaaga cataactcat taaattaaag ggatgcaaat
    55921 gaataagttt atcgctgctg aagctgcgga atgtataggc tgtcatgctt gtgaaattgc
    55981 ctgtgcggtg gcacacaatc aagaaaactg gccgctgagt cacagtgact ttcgaccgcg
    56041 tatccacgtt gtagggaaag gccaggctgc gaatccggtg gcctgccatc actgcaacaa
    56101 tgccccttgc gttacggctt gtccggttaa tgctctgact ttccagtccg atagcgtaca
    56161 actggacgag caaaaatgta ttggttgtaa aagatgcgca atcgcttgcc cctttggcgt
    56221 cgttgagatg gtcgatacga ttgcgcagaa atgcgacctt tgtaaccagc gcagttccgg
    56281 cacgcaagcc tgtattgaag tttgcccaac acaggcgtta cgactgatgg acgataaagg
    56341 gttacagcag ataaaggcgg cccgccagcg caaaacggca gcaggaaaag cgtcatcaga
    56401 cgctcagcca tctcgcagtg cagcgttgct ccccgttaac tcgcgtaaag gcgcagataa
    56461 aatttcagcg agtgaacgga aaacccactt tggcgaaatc tattgcgggc tggatccact
    56521 gcaagcgact tatgagagtg accgctgtgt ttattgtgcc gaaaaagcta actgcaactg
    56581 gcattgcccg ctgcataacg ctattccgga ttacatccgt ctggtacagg aaggaaagat
    56641 tattgaagcg gcagaacttt gccaccagac cagttcctta ccagaaatct gcggcagggt
    56701 atgcccacag gaccgtcttt gtgaaggcgc atgtactttg aaagatcact ctggcgcagt
    56761 ctctatcggt aatctggaac gctacatcac cgataccgcg ctggcgatgg gctggcgtcc
    56821 cgatgtcagc aaagttgttc cccgtatcga gaaagtggcg gtgattggcg ctgggcctgc
    56881 agggttaggg tgtgctgata ttctggcgcg cgcaggagtt caggtcgatg tctttgatcg
    56941 ccatccagaa attggcggta tgctgacttt tggcattcct cctttcaaac tcgataaaac
    57001 ggtattaagc cagcggcgag agatattcac cgcaatggga atcgactttc atcttaactg
    57061 tgaaattggc cgcgatatta cctttagcga tttaacttct gaatatgatg cagttttcat
    57121 cggcgtgggg acttacggga tgatgcgagc tgatctgccg catgaagatg cgcccggtgt
    57181 cattcaggct ctaccgttct tgactgccca tacccgccag ctcatggggt tgccggagtc
    57241 tgaagagtat ccgctgacgg acgtggaagg taagcgagtc gtggtattgg gcggtggcga
    57301 tacgacaatg gattgtttgc ggacttccat tcgcctcaat gctgccagcg tgacctgcgc
    57361 gtatcgtcgt gatgaagtca gtatgccggg ctcgcgcaaa gaggtggtca atgcgcgcga
    57421 ggaaggtgtc gagtttcaat tcaatgttca gccgcaatat atcgcttgtg acgaagatgg
    57481 gcgtttaact gcggtgggcc tgattcgtac cgccatgggt gagccggggc cggatggtcg
    57541 ccgtcgtcct cgtccggttg cgggttcaga gtttgaattg cccgccgatg ttctcattat
    57601 ggcctttggt ttccaggcgc atgccatgcc gtggttgcag ggcagcggaa ttaaactcga
    57661 taaatggggc ctgattcaaa ccggcgacgt tgggtatttg cctacccaga cgcatctgaa
    57721 aaaagccttt gctggtgggg atgcagttca tggcgcggat ctggttgtca ctgccatggc
    57781 cgcaggaagg caggcggcgc gcgatatgtt aactctgttt gatacgaagg catcgtgatg
    57841 aaatcgttaa ttatcgttaa tccggctgac tgtattggct gccgtacttg tgaagtggcc
    57901 tgtgtggtcg ctcacccttc agaacaggag ttgaatgccg atatcttttt accccggctg
    57961 aaggtacagc gactggactg catcagtgcg ccggtgatgt gccaccaatg tgaaaacgcc
    58021 ccttgtgttg gcgcttgccc cgtgggggcg ctgacgatgg gcgagcaggt ggtgcagacc
    58081 aattctgccc gttgtattgg ctgtcagagt tgcgtcagtg cgtgcccgtt tggaatgatc
    58141 accattcagt cgttgccggg ggatacccgg caacaaatcg tgaaatgcga tctctgtgaa
    58201 cagcgggaag aggggccagc ctgcgttgaa tcttgcccaa cacaggcgct gcagttgctt
    58261 actgaaagag aactcaggcg agtccgccag cagcgtattg ttgccagcgg tgagaatcct
    58321 ctgtgaaaga aatctccgcg ctgaaaggcg cggagaatct attagattgc ccaaccaccc
    58381 gcgtaaaatg cgaccagtgc ggcagtaatg atcaccgtac caatattcag cttttgccat
    58441 tcgcgtgcaa agacgcggcc tacgaccagt gtcacaaagc ccagcataat accggtaacg
    58501 atattacaag tcagaacgat gaacacggca cacaccaggc cagccatagc gtcaataaaa
    58561 tcattgaaat ccagcttcga gacgttactt aacatcagca aacctacgta catcagagcg
    58621 ggtgcagtgg cgtaaccagg gatcaaaaat gacaacggtg ataagaacaa aatcagcagg
    58681 aataacgccc ccactacggt tgcggtcaac cctgttttac ccccggcggc ggtacctgcc
    58741 gctgattcga tataaaccgc tgcgggcgct gcgcccacca ggccggagaa tattgaactt
    58801 actgagtcac tggtcagggc tttgccgccg ttgatgatct ggttgtcttt atccaacaaa
    58861 ttcgcctgac cggcgacggc acggatggtg ccagtagcgt cgaacactgc ggtcatcacc
    58921 aatgccagta cactcggaag tacagttggc tggagtgcgc ccataatatc gaggctgaaa
    58981 atcagagact taccatcttc gccagtcagg cttggcatcg ccaccagacc gtggtatttc
    59041 accgctggat caaagattaa gccgatgatc gaaattgcaa taatcaccaa caagatcccg
    59101 ccgggtacgc gacacttctc cagaccgaag atgaccgcca gccccagcaa gctcatcatc
    59161 accgggaagg aggtaaacgc accgagcgcc actggcaagc cttcaatcgg gtttttgata
    59221 accattccca caccgttagc agcaatcagc agcagaaaca gcccgatacc gatacctgta
    59281 ccgtgagcga tccccatcgg taaattacgt aagatccagg tacgcacacc ggttacggaa
    59341 atggcggtga agatgacgcc catcagaaat accgcgccca gtgcgacagg aacgctaatt
    59401 tgttgcccga gtaccagact gaatgcggta aacgccgtca aggaaatcgc gcaaccaatc
    59461 gccattggca aattagccca taaccccatc agcaacgagc cgaagcccgc gaccagacag
    59521 gtggcaacaa acacagctgc gggaggaaaa cctgctttgc ccagcattcc cggaacgacg
    59581 ataacggaat aaaccatggc cagaaaggtc gttaagccag ccagtacttc ctgacgaacg
    59641 gtactgccac gagcggtaat tttaaaataa ttatccagcg cgccctgtgg ctttggtgcg
    59701 tccggtgttt gtaggatgtc tccagacata atgatgtcct cacgaggatg taaaagtttt
    59761 attattagtt gcgttcgtac accagacgac catcaacgta ggtgcggtag atcgaacggt
    59821 catcgcccaa cgtcatcatc acgaacaatt tgtcgactaa agaaacagag ttgtcatagc
    59881 gcagctgctg tagcggagtg gcggtgggtt ccatcaccac gaaatcagcc tctttgccag
    59941 gtaaaaagtt gccaatcaaa tcgtcaaggc ccagagattt cgctccgccg agcgtggcca
    60001 ggtaaaacgc ttcatatgcc gagaggcgat agccttgtaa ttgcaacact ttgtaggctt
    60061 cgttcagcgt ttgcagcatg ttgaaagttg ttccggcacc gatatccgtt cccatgccca
    60121 ctttgacttt cttctgccat gcttttttca agttgaataa gccgctgccg aggtaaaggt
    60181 tggaggtcgg acagaaagca atgctggatt tggtttcgct gagacgatcc cactcttttt
    60241 cttcgagatg gacgcagtga gcaaagacac agtttttacc ggtcaggccg tactgatggt
    60301 aaacatccag ataaccatca tggtcaggat aaagcgattt cacccaggca atttcatctt
    60361 tgttttcaca gagatgggta tgtacccacg tatccggata ttcctctttc aggcgttgcg
    60421 ccatcgccat ctgttcagga gaagaagtag gggcgaagcg tggtgtaatc gcatatagca
    60481 gacgaccatt tttgtgccag cgttcaatca gttctttgct ttggtgatag ctgctttcgg
    60541 cagtgtcgag cagataatcc ggtgcgttac ggtccatcat caccttaccg gcaatcatac
    60601 gcatattgat atgactggcg gcttcaaaca gcgcatcaac ggattgcgga tgaacagtgc
    60661 caaacaccag cgccgtggtg gttccgttac gtaaaagctg cttgatgaag aacgccgaca
    60721 tttcgcgggc gtactctaaa tcctcataac gacgttcagt agggaaggtg tgtttattca
    60781 accactccag caattgttca ccataggccc ccaccatttc actttgcgga taatggatat
    60841 gtgtatcgac aaagcccggt actatcagtt tgccgcgata gtcgcgcacg cgaatggtgt
    60901 caggaatttg atgctttccg ttttcccatt cgccaaacca ttccactttt ccctgtttaa
    60961 tgagtaataa accatcctca ataaaccgca gcgcagaggc aatctcttcc gggttatcga
    61021 ccgtacgggt gacatcaata aaactgcctc gtaccgcttt taacgtgtgt tctcctgaca
    61081 tcattgactc ctttactgtt aatccatctc ttccgctgag gtaataccag gcagaacgtt
    61141 ttcctgtcgg tagccaccag ggaggataat atttaataaa atcgcagtta acccgccagc
    61201 acaaataggg ttttcaacta atacataaat agaggctggc aatattttaa aaatttcggg
    61261 atcgtaggag acgccaagcc ctaaacctaa agaagtggcg acaataagtg tttcacgccg
    61321 ctttaatccg ttggtgatga tgatgcgaat cccagcgatg gcaatcatgg aaaacatcaa
    61381 cgtcattgcg cctcccagaa ctgccgaggg aatggtcgtg aagaagccgc caatcatcgg
    61441 aaataagccg aggataacca gcattaccgc gatggttcgc ccgacataac gtgaagcgac
    61501 gccagtcatc tgaataaccc cattattttg cgcaaacgtg gttaatggta atgaaccaac
    61561 agcggaggcg ataacagaaa ccagaccatc tgccagcacg ccgcctttca gtcgggactg
    61621 atactcttcc ccctgaatgg ggcggcggga aaccattgcc gtggcggtga tatcgccgac
    61681 tgcttccagc acgctaagca gataaatcgt gccaaccacc aggaactgat ggaagctaaa
    61741 actaaagccg tatttgaacg gatgtgggat ggtgattaac ggcaaattgc gcatactgct
    61801 gaaatccacc atgcccaggc ataacgatgc gatatagccg acacatagcc caatggcgat
    61861 ccctcccatg cgtagcaacg gactgcgaca gcagttaaag ccgatcacca caatcaaaac
    61921 caataaacca acgccgagat gttcgtaatt gccgaacgta ccgctgcttt tggctgcaaa
    61981 tcctccacca aaatcgataa tgccgacttt aatcaggctt aagccgatca tcagtaccac
    62041 aataccgctg acagtaggcg taataacccg acgtaaatag ggcaggataa aagaagagcc
    62101 gacaaccaga aatgcgccaa cgaaggagac gccgagaagc gacgacatga ttaactcttc
    62161 gtgaaaaccg tcgcttttca tgctgctgcc cagcgcaatc atgaccgtaa caaatgaaaa
    62221 attgactgac tgaattgaga gtaggccaga accgacgatg ccgtagcggt ttacttgtaa
    62281 ccaggtacca ataccagagg cgatcatcgc catagaaaca agataggcgg ttgtttcagc
    62341 ggaaagctgt aaggccgcac ccacgattaa cgcgggggtt accatcggaa cgaaaattgc
    62401 caacagatgg gtaatggcac caacgagagc ctgatgaaag ggagggcgat cctccagttc
    62461 aaatataagg tcagaacctg catggtttat atcagacatc ccttcactcc ttttaaagta
    62521 aatttgatca tctgaattag taaaataaat tcccacgaat ttggggcgca ggacgccttg
    62581 agaacggctt atctcatttt cgcagtcact atttagtcat cgttcaaaca gagaaacagg
    62641 ccgccgaagc ggccatttat tatttatatt ttttccagcg cagtgagtat tttctccggt
    62701 gtgaaatgcc attcgcgtaa ccagatgccg catgcatcgt gaattgcggt agcaatcgcc
    62761 ggagctgcgc catttacacc gatttccgag atcgatttcg ccccgaacgg gccgacttta
    62821 tcgtcgctcg gcaccagcac agcgtggaaa tcacgcggaa tgtcaccaat tttcggtgcg
    62881 ccataactgc gtaaatcacg cgttaacggg tgaccttcgg cgtcataaat gatctcttcg
    62941 ctcatactgt ggccgatagc acgcagggtg gcaccgtaga tttgtcccaa cgctaactct
    63001 ggattgaccg gtgtaccgca gtccagcaag gcgtagaatt tatccaggcg gatttcaccc
    63061 gtacgcgtgt tgacggcaac ttcagcgaag tttgcgccat acgggaaagc gaaatcaggc
    63121 gtgatataac tgccagttcc caccagtgaa ccaaagccgg tgccggtttc gcctttatgg
    63181 gcaatatccc cgaaactaac ttcgcctttc ttgccgcgca cgacgcccgg cgttgctagt
    63241 tgaacatctg ccactggctc acccaacatt tgcgcgccgt ggaacagaat tttctcccgc
    63301 agattttccg ctgccaaacg cgccgcgtta cccgagaagc aagtaccgga cgaggcatat
    63361 gcgcctttat caaacaacgc atgatcggta tcaccggaga taacatgcac gtcctgcggt
    63421 gggcagtgca gcacttctgc tgccagtttc gtcactacag tatccagacc agtaccaata
    63481 tccgcaccgc cagaatgaac gataaaggtg ccgtccgatt ccagtttgat catgcagtta
    63541 gcctgatcga tatccgggat acccgatttc tgcatgataa tggcgacgcc gcgaccgatg
    63601 tgccagtcac cattttgcgg tttcggtgaa gaccattgga tcatctcgcg accctgacgc
    63661 aggatctctt ccagtgcgca gctggcggcg gaaggaacgg aggttggcgc tttaccttca
    63721 ccaattgcac cgagaatttt cagctcttgc ccttcgtgta cccggttacg ttcgataatt
    63781 tccagttggt cgatctgtaa ctgttcagca agttccgcta atgccatggt gatagcgaag
    63841 ttacctttcg gtgcgccata accctgataa gcaccatttg ggcaaatgtt gctgtagtag
    63901 gtggtgacct ggaaatcgac gttatcgcac ggatataagg gcagcgacag cgccggtccg
    63961 ttacacggta cggtgagtga gtggttgccg taagggccag tgttggcgcg gaaatccatc
    64021 ttcactgccg tcaggcgacc atcttttttc gctcccagtt tgacggtgac tttcgcgacg
    64081 tgacgagagg tgttagcaat aaactcttct tcacgggtgt agcggaacag taccggacgc
    64141 ccggtcacgc aggttgccca tgcgcacact tcttccagca ggatgtcctg tttggaacca
    64201 aaaccgccgc caactcgctc tttaatgaca tgaactttat gctgtttcat gcccacgagg
    64261 cgcgcgacct ggcggcgtaa gtgccatggt acctgggtgg aggcgtggat aaccagacga
    64321 tcgccatcca tgcgggtaaa gcagatatgt gtttcagtcg ggcactgctg cgcctgcgtt
    64381 gagttatagg ttcgctcaat gatcacatcg gcatcggcaa agcctttgtc catatcgcca
    64441 atatgaccat gaatactggc ggcgatattt ttgcgggggc gagaaccgat cgggaagtta
    64501 atgatcatat gctcgccgcg ctgggctgca tggctgttat cgtcttccag agtatctggc
    64561 gcaccagcaa catacaccac cggttcatcg tgcacgacag gcgcatcttc cgccattgct
    64621 tcgtcgatcg acattaccgg cttaagcact tcatattcaa cgtcgatgag cttcagtgct
    64681 tcgagcgcaa tttcttcact ttcagcgacg actgcagcaa cacgatcgcc gacgtgacgc
    64741 attttcttgc cgaacatacg gcggtcaagc ggtgacggtt ccggtgcgct ctgaccaccc
    64801 ggggtatagt agatatccgg gcaattcagg tgagtaataa cgtgaacgac gcccggtaag
    64861 gcttcagctt tgctgacatc cagatgagta atcagtgcgt gagcgtgtgg gctacgtaac
    64921 attttaatga cgcaggcgtc agccgttacg cggtcttcaa catagcaggg cttcgcctgc
    64981 accatttttg cggcatcagt tttaggataa tgcttaccaa tgacttcaag gtcgtcacgg
    65041 aaagtcggag cgatatcaat ggtggcctgc ggattatttt tacgtgcaac cgccagttca
    65101 atgacctgat agtattgctg ccagccagca tcgcggctaa acagacctga gagcgagtcg
    65161 tcgatctctt cgcgggtagg tgcggcgatg cgatcgagaa gatcggtgat aatcagagcc
    65221 gcagctggat cgttataacc agactgcacc acgccaacat caaccattgc ctgttgaacc
    65281 agacttaact cgttccattt gcccagagat tctgcggtac gaatatctgc cttctctaac
    65341 tgtgcggcaa taagcaagga cgcgttaacg atattaccgt taaagattat tgcgtcagaa
    65401 ccggcaaacc cgaagccatc atcactgttg cgtacagagt gcattcccat gttaaacaac
    65461 agcttttgca cgttttcgcc tggattaacg gttagctcct gaggcgcgcc atttaaagta
    65521 aagtggatga tcatacggct tcctccccag cctgttggca gtcggcatac aggtcggcta
    65581 ccagaactcc cgtgatatag cgtttatagg ccacgctgcc ccgcaaatct tcctgcggga
    65641 aaatggcgtt ggcgacagcc tgttcaagtg cattgccttc cagattttgt ttttcgacat
    65701 cgtgcagacg cagtgcttta ctggcgacgc catccagcgc aattcgcata ccgtcgtggt
    65761 ctgtcattgc aacggctgcc gtcacgacgg ttaaacctgc ctgagaacgg ctaattttcc
    65821 gggtcgcaca ggtgcgatac ggatctttaa tgataatttc ggttagcagg cgatcgcatg
    65881 ggcaggccag gtagtcctcg attgacagcg tttcgccgtt gccaaaaacc agttcagcat
    65941 ccagtgccag caggacggga agcagcaccg actcttcctg gcgggcggcg atttcaccac
    66001 caatggtcga ctgattacga acatggcgtg agtaaacaaa accgagggct tcacgcagcg
    66061 ctgcaggaat aaatcgcgca tcacgcagtg gctgcaagcg agacattgca ccaatccgca
    66121 gtgcaccgtt atcccagtca acccagtcca gttccagatc ctgtaaggaa atggcaatct
    66181 ttttatcggt acgggttggt gtagcgttga gtttgctgcc cccggcgaac cagacggctt
    66241 catcctggta gcggcgcttc agttccagcg cctgttcgac agagtcgggc ctgaaaaatt
    66301 gttcaatcat cttctttcct tatttaaccc tcttccctgc gggaagaggg catctgtcat
    66361 ttatgccagc gcatccatcc gacgccacat actggcagcg gcttttctgg cttgtgcata
    66421 aatggaatcg caatcgaagt taaactgacg gtcttcatag accatcacac cattgaccat
    66481 cacgctgtga acgctgcctg agcccatacc gaaagcgata tgcccggcga tattgtctgc
    66541 cagcagcggc gtcggcgagt tgtaatcgca aatggttaaa tcagctttgt aaccggcttc
    66601 cagaagccca aatttcgcgc caaagttgcg gctcatcagt tcgttaccgt tagtcagggc
    66661 tttggcaaaa ctgtcaggcc acagcggacc acccgcatcg cgatgtttaa agaaggcaaa
    66721 tttcatctct tcaaacatgt ccgatccaat gccgtccgtt cccaacgcca gattacggat
    66781 gtcgctaagg tgatggttgt agccgacatg gttgttcatg tttgaacggg cgttatgcac
    66841 caggaacgca tcgcgttgat tgagtagggc gatgtcatct ttcgacaagt acagcccatg
    66901 agcgaccagc gttttgctgt cgatgagatc gaattgcgcc agtcgtgcca gtaggtcttt
    66961 gccgtaccag tggtgactgt aggaaacgtc gtaaaggtct tccgcagcgt gaatatgcaa
    67021 accacggcct gtggctttca cggcttcacg cagcatctcc agaccggcat ctggcacggt
    67081 aaacggcgcg tgagcaccga tatgtgcttc caccagatac ggctcggttg ctgctttctt
    67141 cgcctcatca atctgacggg cgaagcggat gttttcttct acaccttcct gcaactcttt
    67201 gatgccgttg ttacggtcag tagtttcaaa acaggtcatc gcgcgcaggc caacttttaa
    67261 aaatgcgtcg cgcaatgtgg agagcgaccc gccgatatac gccggagagg catggtgatc
    67321 gataaccgat gtacatccgc tcttaatcgc ttccagggaa caaatcagtc cgctgtaata
    67381 gagcgactct tcatcaaggg cgcgatcgag ccgccaccag agatttttca gcgttgagat
    67441 gaaatccggg caaggggcga tgtttgccat aattccgcgg gaaagccccg agtaaaaatg
    67501 gttgtgcgag cagacaatcc ctggcatcac aatccggcca tgcatctctt tgaagctggc
    67561 gtcggggtag cgttgcgtca gggcatcgcc gatagcgaca atcacatcgt tttcgatggc
    67621 gatatcaacg ccttcctgca cttttgccgg gtgtagctgt accgcagtga cattcttcag
    67681 aatcaacatg attacacctc cacgcggccc agcagataat gatgatgctg gtggacatgg
    67741 ctgatgatgc ggcacatatc gttcagctcc ggtggtacgt tgttaaactg acctttgctg
    67801 tcgatgttta acacccagct ttggttattc agacgtactc gtacccggca atcttccaca
    67861 aggaagcctg ggttgctgct gttatcaaag tcttgcgcca ggctgaagac ggtgattttg
    67921 tctttgtacg gtttaccgtt ccacggacag aactgagcgc agttgccgca ttcgttacag
    67981 taagcgtcga ggtgcagcgt ctggaaacgg ttctggaagc ctgggaccgc aatggagacg
    68041 ttggcgcggt tcgggcagac atccacacac ttgctgcaaa cgtagttaca ttcgaggcag
    68101 cgagcggctt cctgggcgac aaacgcgtca cgatcgtcac tgttcaccag cgtgatagag
    68161 atatcgcctt tacgttgata gatttccgct ggattgacgt tgttccagta tttatcgttc
    68221 tggtgggaac ggatattttc ccgactaagg atggcatcgg tcgcccgacg cgcggttccg
    68281 acagcagcga caatggagga tggtccgcgc tgtacgtcgc cgatcataaa gacgtcagtc
    68341 agacgagttt cgccattatg gtcgacgtct ggccagccgt ttttgtccag cggcacgccc
    68401 atcgcattca gggcttcagt atcctgctgt tcaccaatgg cggtgatcag gctgtctaca
    68461 agcagtgtta ctgtttcgtt ggtttcaacc ggacgacgac ctttctcatc cggttcgcca
    68521 agcgacataa cgcgcaaggt taaggtgcca tcagcatcga aacgttccgg attgttcagg
    68581 aaacggaact ccacgccgtc gtgcaacgct tcttcatact cttcgcgcca tgcgggcatc
    68641 tcttgcagtg aacgacggta aacgatcgtt gctttttcta cgcctggaac gcgtaacgcc
    68701 gcacgagcgc agtccattgc ggtgttaccc gccccgacaa cgaccacatg tttgcccagc
    68761 ttgagcgctg tacccttgtt gtattcacgc aggaagggga gtgatttcca gacattttgg
    68821 ttgtcgcccg ccagtttcac accgctattt ttatcagtgc cggtggcaat cagaacatag
    68881 tggaagccct gattttttaa ctgttcaacg gttaaatcgg gtgagcagcc atactcaaat
    68941 ttcacgccgt gatcggcaac aaaatcgata tcgtgctgaa ttaactctgc aggaatacgg
    69001 aactgaggaa tgatattttt cactacgccg cccgcattgg cttcgcgttc aaacagcgta
    69061 accggatggc ccgctctggc aaggaagtaa cctgcagcca gacccgccgg acctgcacca
    69121 atcacggcaa ccggatggcg tgaaccagaa ccggctggtt tgtgccagcg ttgcttatat
    69181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag tgcactgtcg
    69241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggcg
    69301 ggcagggcgt tgcgttggta gataagttcc agcgcgtcgg catagcggtg ttcgccaagc
    69361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
    69421 tagcagtcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
    69481 ttctgggtgt attccatggt tagcgcgtct gctgccagtc tgttcagtcg ttcgacgtca
    69541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
    69601 agatagccac caggtttcag caggtcggtt gccatcgtga tagggcgaat accagtatca
    69661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
    69721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
    69781 gacatataca tctcttcacc gggcagtgcg cctttattgt tgatggtgcc gagagtgtta
    69841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtgccatcag gcgttccagc
    69901 atttccagcg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
    69961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggttggg
    70021 ttaagtttca caaaggtgtt gagccctttt tcttccagca tgtaacggca aatggcttcg
    70081 atttcatgcg gaggacagcc gtgcatggtg gagagggtga cgccctgcac catactggtg
    70141 gggatgcgag cgggtaaagc ttgcaagctt tcgcgttttt cctgcaatcc gtggcgggct
    70201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgcgc gaatttcgga
    70261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
    70321 tcgaggttgt aaccaacgct catattaaag atgaacgatt taccggaatc agaaggctgg
    70381 aagatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
    70441 agcagggtaa attcggtaga ccattcggtg ttaaagcact cgtcttcggc atcgatacag
    70501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
    70561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
    70621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacgggtgag
    70681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
    70741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
    70801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
    70861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggcggct
    70921 cgaaacttaa gggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
    70981 cctgaaaatt attattgcca gcccatttat cactttttct cttttctcaa aattagtgat
    71041 tccttatcat tatgataaag agtgctggag gggttaaata tttgaacgca atcgtttaac
    71101 tacgttttct aaatttcagt ataccttttc ttcgctgtaa taaagtcgtc cggagtatca
    71161 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
    71221 gcctgacgca tattggtgac attgggtcgc tggaatgcct gcatcaagca tggttttgac
    71281 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
    71341 aacgaccaga tttttctaaa aatatcaatg gtgagggttg gcatgtcacc gtgggtgaga
    71401 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga ctgatgttag taaaccctgg
    71461 gcataatctg ggttgtgaat aatagtgata ttactctggt tcgcataacg ttcgtgcagt
    71521 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
    71581 atacttgtat caagaattgt tccctgttgc cagggtaaca tcattttcca ttgccccatt
    71641 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
    71701 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttgggggt
    71761 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattat
    71821 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
    71881 aaaccagctt gcttttttgg ctggcagaac tgctccagac tcgcggcagg cgtgtgttaa
    71941 tcacgacgac gacacatatg tttatgccaa agtcacactg gcccgtggtt ttctgtcgtg
    72001 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
    72061 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
    72121 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
    72181 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
    72241 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
    72301 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
    72361 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
    72421 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
    72481 tgcaacaaca tgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
    72541 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
    72601 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
    72661 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
    72721 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
    72781 tgaagagtcg cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
    72841 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
    72901 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
    72961 agcccagagt gcggcgctat taggatttga tattgccgtt gctgatattt atcgcgaaag
    73021 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
    73081 ggcagtggaa gcactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
    73141 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
    73201 aagtcgtcgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
    73261 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
    73321 gatcgccatc agcgtgctgg cagaaatatt acaggtgaaa aataacgcgc cgggtgggct
    73381 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcggggga
    73441 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
    73501 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
    73561 cgaaatgacg gtcgaaggcg tcactgcccg cctggcaacc agctctgcgg aagcgatgaa
    73621 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
    73681 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
    73741 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgctggga aggattgtca
    73801 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
    73861 gcaggagaat accggtgttc ctggcaatat tatggggcat accacccgac gggtgatccg
    73921 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
    73981 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
    74041 tggcttgttg aacgacggac tggcagtggt cggtggtttt aaaattggtg atatcgatcc
    74101 tcgtggtgaa acggctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
    74161 cgtacttgaa gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
    74221 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctga gactgatgac
    74281 aaacgccaaa ttgcctgatg cgctacgctt atcaggccta caagtgcatt gcaatctatt
    74341 gaatttgcga tgttttgtag gacggataag gcgctcgcgc cgcatccggc atcaacaaag
    74401 cgcacttact tgacaatctt tggccggatg gtattaccca cccggccttt aaattactga
    74461 caaatcaccg ttcctgtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
    74521 agtgcttcac gacctgcgcg agagcgagca aacgaggcgg cagcttccac tttaggcagc
    74581 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcaca taatgacagg
    74641 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
    74701 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
    74761 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
    74821 ccgccaacgg tgatcaccac ctgacctgca tctaccagag ctttaaccgt ttctttttca
    74881 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
    74941 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
    75001 atcggcttgg tcgggttgag gaatgccggg tcgttagcat cgacttcgac ctgggtaacc
    75061 agcgttgcta caggtttatt aatgccacga gaaagcagtt cttctcttaa tgcgttttgt
    75121 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
    75181 gagtgcgctt cagtttttgc ggcagcttca aaggcctggt taatcatgcc cacctgcggc
    75241 ccgttaccat gagtgacgat aacttcatgt ccctgggcaa ttaaatcaac aattgcctga
    75301 gaagtaattt ttaccgcttt catttgcccg gccaggtcat cgcccagcgc attcccgccc
    75361 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
    75421 tccctggcag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
    75481 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
    75541 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
    75601 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
    75661 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcatcgcgc
    75721 tggttaattc aacaaaacgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
    75781 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
    75841 tagaaatttg caggcgttga gccatcgaga acgtgcagtg atcggtcgct accacgtcaa
    75901 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
    75961 ggctaagaat gaacttcatg ccatcttctg tatcgtagct gcgttcgtcc aacaggagat
    76021 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
    76081 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
    76141 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
    76201 gatagcgcgg tgcgattaac cctgcggcga taaattccgc ccgcttgctg gcgatagcag
    76261 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcagacgg cgtaatgcct
    76321 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
    76381 acccttcctc gaccatcata gggatttcgt cgaggatggc gtgattaatg tgctggatca
    76441 caccgtgaaa gctgtagtcg atgaccgcct tatgggcggc atagccacga taaacctcca
    76501 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
    76561 taccgccaca cgcagctgcg cgggtaccgg taaaaaaatc atcacaactg cgcgcgatgc
    76621 cgacatcaat attgaaatgc gtatggacat ccacgccgcc agggaaaacg taacagccag
    76681 tggcatcaat ttcttcatac gggagctgcg gcgaaatatt gttgcccaac tggcgaacaa
    76741 tcccgctttc aatcagcaaa tcctgtttgg cttgtccatc tgcgttaacg acagtgccgt
    76801 ttttgatcaa tacgcgcata gcaaactcca aaaaccctcc ggcgaaccgg aggggaataa
    76861 gtgaagagta attattcggt tgccagccag cttaacggga ttgcagcgta catcgcggca
    76921 caggtcacca agtgagattt ccaggttttt tcgttaggtg catgcgcttc aggttcttta
    76981 cccgggccaa agccgatcac cggaatgccg tgacggccca tgatagatac gccgttagtt
    77041 gagaaggtcc acttatcaac aaccggcgct ttgccaaaca gcccttcgta ggcattcacc
    77101 agtgctttaa cggtgaagtg atcttcttcc actttccagg tcgggaagta gcattcggtt
    77161 gggtaaacca ggccagtcca ggacggacgg tcgtagttgt acatagaaac aaccgcgtta
    77221 gctttctgta ctgcaggcag ggcgcggatt tcgtccagcg cgccttccca ggtttcgccc
    77281 caggtcagac ggcggtcaat agagacggcg cagctatctg ctacagcgca acggcttggg
    77341 gatgtgaaga agatttcaga aacggtgagg gtgcctttgc cgaggaattc gtcataaccc
    77401 agacgttggg agagttcttg taattcgcca agaatcggac ccattttgaa aatggcgttg
    77461 tcaccgcgtt ctggcgcaga accgtggcag ctaacaccct gaacatcaat acgaatttcc
    77521 atacgaccgc gctgaccacg gtatacctgg cagtcggttg gttcggtact gaccacaaat
    77581 tccgggcgaa tgccggattg ttcaataatg tactgccagc acagaccgtc gcagtcttct
    77641 tcctgcacag taccggtaac cagcagggta tattcatctt ccagaccgag gtctttaatg
    77701 attttaccgg cataaaccat agatgccatg ccgccttcct ggtcggaagt accgcgacca
    77761 ccgatcagct catcagtttc catgccttcg tacggatcga agtcccagtt tttgatgttg
    77821 ccaatgccga cggtatcgat atgagcgtcc attgccacca gacgcgggcc gtggccgata
    77881 taaccgagaa cgttacccat cgggtcgatt tcaactttat cgaagccgac tttttccatc
    77941 tcttctttaa tacgatgtac tacgcgtttc tcgtcgcagc tttcactggg aatagcaacc
    78001 atgtcgcgca ggaagcgagt catgtccgcc tggtaatctt ttgctttttc aagaatcagt
    78061 ttgaatggaa tattcttagc cattattttt ctccagattt tttactcccc gttttgcagg
    78121 gagtagttcc aatagggtga ttaaggtgct acagcgtgtt tgccttccca gacaacttcg
    78181 cggtagtgct tcacgtcggt gtcgccttca gtgctgataa ccagcactac ggcatctttg
    78241 ttcagcgcca gtttttccat caggctttga cgttgcgggt gataatgaac cgccgcgaga
    78301 acgcccaaac cgacagcgcc ggattcaccg gagatgatgc gcgggtcgtt gccgtacgga
    78361 ttacccagca cgcgcatacc taatgcggca acgctgtcct ggcaggagat gaattgggtg
    78421 gcgcagttac gtaggatttc ccagcccagc gggttaggtt cgccacaggc caggcccgcc
    78481 atgatggtgg ccatatcacc gccaacgttg acgatgtcgc ctttgacgcc ggagcgataa
    78541 atacagtcag ctttgtcagg ttcaacaata atgctgtgca gattttgcgg gctatagacg
    78601 tcgaccagat aacccagcac accaccggcc attgctccga caccggcttg cagcagaacg
    78661 tgcgtcgggg ttacgcccat ttcacgcatt tgctcgacgg cttcatctgc cagggttgcg
    78721 tagccttgca tgatccaggt tgggattttg gtgtaacctt cccatgccgt gtcctgtacc
    78781 acttcccagc cgtgctgctg cgcgtgttgc atggtcaggc gaacggtatc gtcatagttc
    78841 atatccgtga cgatgcactc ggcaccgagg ttcagaatgg cgtcaacgcg ttcctgagca
    78901 gaacctttcg gcatgtaaat caccgcattc tgtccgagtt gctgcgctgc ccacgccaca
    78961 ccgcgcccgt ggttgccgtc ggtggtggtc gcgaaagtca ttttttcgcc gatggcattt
    79021 ttcaggtgct caaatgacag cgtttcgata tcaagatgat atttttcaca caataattga
    79081 gcgatagcgt acgcaccgcc gagcattttg aaggcgttca gaccgaatcg ttttgattcg
    79141 tctttgacaa gaattttctt cacaccaaaa aggtttgcga gatcgtccag cgcacaaagc
    79201 ggtgttgggc gataaccagc tattttctgg tggaactggc gcgccagttt ggcctggctt
    79261 tgcgaaaaga gcggtgatgt ttcgccgttg aaaaatttgt tatcggcgat atcaatcttc
    79321 aatgagaaaa cggacatatc ctatcctttt ggcctctgga aatagagggc cgaccagctg
    79381 atcggcccga ggggttattt cacgcgttct tgcgcttctt tcagcagttg ctcgagcagt
    79441 gcacctggtt tggcgtattt acgggacagg atcatcgcgg cgatgatata aggcttccag
    79501 ctggcttctt tgtaggtagc gatacggtat ttttcgaata cgccttcagt cacttcacct
    79561 tctttacagg atacaccgct gatatcagct ggcaggcagt gcatgtacag ggcttcgcca
    79621 tcacgggtca gttccatcat ctcttcagta caatgccagt ctttgtgttg cgcgttctgt
    79681 gccagacact gtttttccag tgcttttaag ccttcgtgat cgttcgcacg cagcaattca
    79741 gtacgctctt ccatcacttt gtaaggtgcc catgacttcg gataaacgat gtctgcgtct
    79801 ttgaaggctt cttccatgct ggtgacctga cggaagctac cgccggaggc tttagcattg
    79861 tttttagcga cttcaacgac atccgggatc aggtcgtagc cttccggatg ggccagggtg
    79921 acatccatac cgaagcgagt catcagaccg atgatgcctt gtggtacaga gagcggtttg
    79981 ccatagcttg gggagtaggc ccaggtcatg gcgatttttt tacctttcag gttttccaat
    80041 gaaccaaagt gttcacgtaa ccatgccagg tcagccattg actgagtcgg gtggtcaata
    80101 tcacattgca ggttcactaa agccggacgt tgcggcagca caccctgctt gtaaccgtca
    80161 tcaagtgcag cgccaacttc acgcatatag gcgttgcctg cgcccagata catatcgtcg
    80221 cgaataccaa tagcgtcggc gcagaaggag atcatattgg cggtttcacg cacggtttcg
    80281 ccgtgagcga tttgtgattt gccttcgtcg agatcttgct gtgcaaggcc gagcaggtta
    80341 agcgcggaag cataagagaa gcgggtacgg gtggagttgt cgcggaatac ggaaatacct
    80401 aatccactat taaagacttt ggttgagatg ttttcagcac gcagtgcttt taatgctgcg
    80461 gcaacgtcca gtacttgttt cagttcatct ggcgtctgtt cccacgttaa caaaaaatct
    80521 ttctcgtgaa ggtgagaggt cagcgaattg atatccttaa tcagctcatt aacagttttc
    80581 atcataaagt cccggtagtg gaatgacagg caaagtggtt tatttcactg tgacttgcca
    80641 aagtgattgg tgttttcaca gctatacctg ggactaacaa taagtgtgcc agaattatcc
    80701 acggggctgc aaaagactaa tttgacggat aatgtgtggg gttgatcaca aattgatgct
    80761 agcttccggt tgtttgtaaa atacgaaaac gtttggcttt ttttattgcg ttatttattt
    80821 gctgctaagt atcagaaata agataaaggg gcaggctatc cctcgtcagt ttgataaact
    80881 caccgccgta attctcatat tgatatagaa atgtgatatc tcttccataa tttcattaga
    80941 aagtgataaa aatcttatgt ttttatcaaa gccaatttcg cctgagttaa tatcatcatg
    81001 atctgtgaac catcaacgtc ttcaatgatc gacttttaag taagtctgga aggtaaagct
    81061 atggagcttg ctactacgca gtcagtattg atgcaaattc aaccgacaat tcagcgtttt
    81121 gccagaatgc ttgccagcgt tttgcagctt gaggttgaga tcgttgatga aaacttgtgt
    81181 cgcgtcgccg gaactggcgc gtatgggaag tttcttggtc gccagttgag cggcaactca
    81241 cgcctgctcc gccacgtcct ggaaacgaaa actgaaaaag ttgtgacaca gtctcgcttc
    81301 gatccccttt gcgaaggttg cgatagtaaa gaaaattgcc gcgaaaaagc atttctgggt
    81361 acgcctgtca ttttacagga tcgttgtgtt ggggtgataa gtttgattgc cgttacccac
    81421 gagcaacaag agcatatcag tgataattta cgcgaatttt ctgattatgt tcgccatata
    81481 tccaccattt ttgtttcgaa acttctggag gatcaggggc caggagataa catcagtaaa
    81541 atattcgcga ccatgatcga taatatggat cagggcgtat tagttgttga tgatgaaagt
    81601 cgggttcagt ttgttaatca gactgcctta aaaacacttg gtattgtaca aaataatatt
    81661 attgggaaac ctatccgttt cagaccatta acatttgaga gtaattttac ccatggacat
    81721 atgcagcata ttgtttcgtg ggacgataaa agtgaattaa tcattggcca attgcataac
    81781 attcagggcc gacaattatt tttaatggcg tttcaccaat cgcataccag tttttctgta
    81841 gcaaatgcac ctgatgaacc gcatattgaa caattggttg gcgagtgccg tgttatgcgg
    81901 caattaaaac gactcattag ccgtattgca cccagcccat ccagcgttat ggtggttggt
    81961 gaaagcggca cgggtaaaga agtcgtcgcc cgtgcaatcc ataagttgag cggaagacgg
    82021 aataaaccct ttattgctat caactgtgcc gcgattccgg agcagcttct ggagagcgaa
    82081 ctgttcggtt atgttaaagg cgcatttact ggcgcttctg ccaacggtaa aacagggttg
    82141 attcaggcgg cgaatacggg cacgctgttt ctcgatgaaa taggtgatat gccattaatg
    82201 ttgcaggcta aattactgcg cgctattgag gcacgtgaaa ttctgccgat tggtgccagt
    82261 agcccaatac aagtcgacat tcgcatcatt tctgcaacta accagaattt ggcccagttc
    82321 attgccgaag gtaaattccg cgaagatctc ttctaccgac ttaatgttat cccgataact
    82381 ctgccaccgc tgcgtgaacg tcaggaagat attgaactac tggtgcatta ctttttacat
    82441 ctgcataccc gtcgtctggg atcggtttat cctggcattg ctcccgatgt cgtcgaaata
    82501 ttgcgtaagc atcgttggcc cggaaacctg cgcgagttaa gcaatttgat ggaatatctg
    82561 gttaacgttg ttccttcagg tgaagttatc gacagcacgc tattgccgcc aaatctgctg
    82621 aataatggca caacggagca aagtgatgta acagaggtca ctgaggcaca cctgtcactc
    82681 gatgatgcgg gcggcacggc gctggaggag atggaaaagc aaatgatccg cgaggcgctt
    82741 tcacgtcata acagcaagaa gcaagttgct gatgaactgg gcatcggcat tgctacgctc
    82801 tatcgcaaga ttaagaaata tgagttgtta aacacataac ctcagtgaat ccggcctgaa
    82861 ttcaggccgg atatcctttt acttcgtttt ctcgcaatcc agaactgtat ttacaatcat
    82921 ctgatacccc gtgcagcgac aaagatttcc cgccagtccg cgacgaattt ccgtaatggt
    82981 taatggcttc tcgcgtggtt tcgccagcat tgccgtggta gccataatca ggccaggcgt
    83041 acaaaacccg cactgcactg cgccggattt cgcataagcc tgctgaacat gagaaagttt
    83101 tccgcctttc gcttcacctt ccagcgtgcg gatctctttt ccttcagccc aggcggcaag
    83161 gtataagcaa ctgtctattg ctgtgccgtc gaccagcacc gtacaggcac cacattcacc
    83221 cacgcagcac ccttgtttga cacttagcag tccttgttcg cggagtaatt ccgagagcgg
    83281 cgtgcctggt gcggcatgaa gctgaaaagg catcccgtta atggtgcatt cgatggtaat
    83341 tgtttcgctg tgattcattg caattttccc cccgccgcgg cgacggcttc gctaatcact
    83401 tttttggtca tcgtctggat gagatgcaga cgaaactctt tactggcccg ccatgaagaa
    83461 cgcggggcga catcttgcag gacagattcg ctgatagctt ccagcgtttg caggtttaat
    83521 ggcgcatttt gtgcagtatg ttcggcatgt tggcagcgaa tcggcgttgg cgcggcaaca
    83581 ccaaatgcca ggcgtaattc gctgaaattg ccgttatcca gtcggcaatg tgcggcacag
    83641 ccaatcgttg aaatatccat tgcgtcgcgc atggcatatt taaaatgcgc gctgcccacg
    83701 tgttctttcg gctggggcgg aaaatgaaag gcgacaagga tttcgtcatg ctcaagagac
    83761 actttgcccg gcccggtgtg aaagccatta atcgggacga aacgaacact gcgtggggag
    83821 tggatctcca gtttcgcgtc ataaatgagc gttggcgtgg cagaatctgc gctggtggca
    83881 ccgttgcaaa tatttccacc gtaggtagcg acgttacgga tctgcggtcc agcaatggac
    83941 gaggccgcag cacataacgc cgggagatga cgttgagtta taggatcttc tattagctgg
    84001 gtaaatgtcg ttgcagagcc gatacgtagc gagccatctt ccgccagcgt aattccccgc
    84061 agctccgcca gattatgaat atcaacaata tggcgataac ggtcattgtg atggtggagc
    84121 tgaatcagta cgtcagtgcc accggcgagc agtttggcct gcgggttgtc agccagcagg
    84181 ttgatggcat cggcaagggt tgctgcgcga tggtaagaag caaaatcaaa catgatgtta
    84241 tcctcaaatc aatcctgcca gatgaaactc ttcatataac cgttttggcg tcagcggcag
    84301 tgtattgatt gcaacaccgg tagccatctt caccgcgtta cgaatagctg cggcaacagg
    84361 aattattggt ggctcaccca gtgacttatg tccgtatgcg gattgcggct cattgatttc
    84421 gacgaacgcg ctttccagtt gtggcagatc cggcatggtc ggcattttgt aatccagcag
    84481 attggggtta cggaccacgc cgcttttcgc atcgatgatc atctcttcaa atagcgccca
    84541 gccaatgccc attcccattc cgccgtgtac ctgaccttct gccagcagcg gatttagaat
    84601 atgccccgaa tcatgaacgt tgaggatgcg gttgatggtg actttgcaca gcgcaatatc
    84661 gaccgtcaga tcaacaaagg tacagccgaa cgccggtggg ttagtggtgg ttttgatgga
    84721 gctttcagca gagagctgcc cgccgcgttc agggtggtag aaagcgtcca tcgccaaatc
    84781 ttttaacgac attaacggct cttccggtcg ttcaaccagc acgatatggc cttttatcag
    84841 ggtcagattc atctctgact gatgtagcat gactgcggcg tgagcgatga ttttctcttt
    84901 taataacagt gccgcactgc gcagcgcagg cgcagcaaca tagctctgac gtgaggcaaa
    84961 tgcgccggga tcgaatggcg taacgtcggt atcttgtgtt gaaataacgc gaacgtcgct
    85021 gaccggaacc cccacggttt ctgccaccat ttgcgagaag acggtgtcgg caccctgacc
    85081 gatttccgtc gcgccgcttt gcacgttgat ggttccatcc tgattcatca gaaggcgcgc
    85141 gcctgctatt tctacgccga caggccaggt attagaggtg tagctaaaac aggcgacgcc
    85201 aacgccgcgg cgtaaattgc cttgctggtt ctggcattct gcacggcgtt tttcccattc
    85261 aaagattttc cggccttttt caagacactc cggcaaccct gcgctgtaaa tacgtttgcc
    85321 cgtgagcgga ttagcatctc cttcgcgggc ggcgttgcgt aaacgaattt caacaggatc
    85381 aatacctaac gctgtcgcgg cgtcatcaag catagactca acggcaaata cgacttgtgg
    85441 cgcgccataa ccacgcatcg caccagccga ggggaggttg gtatagcagg tctttgaact
    85501 gtaagcgtag gcacaacgag gataaaggta agcgacttta ttcccccccg cagaagcgat
    85561 ggagtgcccg tgagatgcat aagcgccggt gttagacaga acatccagac tataaccttt
    85621 caatgttccg tcgcggttca cgcccatttg cccgtcaata gtaaaagcgt ggcgggtacg
    85681 ggttgcgagg aaacactctt cacggctaag ggaaactttc accggaatgc cgccaagctt
    85741 gctggtcagg aatgccgcca ttggctcttc cagtacatcc tgtttattac caaaaccgcc
    85801 accgacaaat ggtttgatga ctcgtacgca tgaccaggga atatccagcg cctgaccaac
    85861 cacgcggcga acaatgtgcg ggatctgggt gctggaaacg atggtaattc gcgagtcatc
    85921 ctccatccac gccagcgatg ttacgctttc catgtgacaa tgttgaataa cgggggtctg
    85981 atagtgcccc tgtacctggt agtcggcggc atcgattgtt tgttggacat tgcccgtcga
    86041 catcgtgctt tgtttcagta aattgccgcc gttatggatt ggtgcagcgt cctctgccag
    86101 cgccgcttct ggcgtggtga taacgggtaa ttcttgccac tcaatgctga ccaattgcgc
    86161 cgctttttct gccgtgagtt catcgcgggc cacgacgatg gcaacggcgt caccatgatg
    86221 acgaacatgg cgagttagca gtgcgcgatc ggcggtatca cgcttgtttt cgtcaagtgt
    86281 ccatgcatgc ccagctgtag cgaatgggat atcaggcaca tcttcccagg taaaaatcgc
    86341 cagtacgcct ggtaaacttc tggcttgttc atcattaata cttacggcat aaccatgtgc
    86401 gatagggcta cgtacatatt tcgcataaca catgcccgcc ataacataat cgtcagtata
    86461 tcgtgcccgc ccggtgacct tagcaatggc atcaacgcgc atgcatgatt cacccgtagc
    86521 ggttgcttcc cgcgcttcca tagaaatccc ctcgatttga acatttggtt cttatggcaa
    86581 agatttagtc taagcaggca agatttacgc cagaaacgac gataagtgtt aggggaggaa
    86641 ggaagtgtga tgacgttaaa aaaacacaga tataatacga aacaggactc tgaatagggg
    86701 cagtgatacg tatcaatatg agtggcgata aaatcaatgt gataaattgg gattattacg
    86761 catcaggatg ggaaataggg taaaaaagtc aggaaattcg atacctctac agcgaaaatt
    86821 ttgctgatga tacttccctt tgccaaatga aattaacctc tttctctatt accgcgtttt
    86881 tccagaagca agagattggg tatcaagagg ctggctgcta tgatatggcg tcttgttttt
    86941 taagcgagga aagattttga gtgcgggacg cctgaataaa aaatctctgg gtatcgtgat
    87001 gttgttatcg gttggactgc ttttggcggg ctgttcgggt agcaaatcat ccgatacagg
    87061 aacgtattcc ggctccgttt acaccgtgaa acggggggat acgctatatc gtatttcgcg
    87121 caccacggga accagcgtaa aagagctggc gcgactgaac ggcatttccc ccccttacac
    87181 cattgaagtt ggtcagaaac taaaactggg tggggcgaaa agtagcagta gtacacgtaa
    87241 atcaaccgcc aaatcaacga ccaaaaccgc atcggttaca ccgtcatcag cggtaccgaa
    87301 atcttcctgg ccgccagtag ggcaacgttg ttggttatgg ccaacgacag ggaaagttat
    87361 catgccgtat tcgacagcag atggcggcaa taaagggatt gatatctctg ctccacgggg
    87421 tacacctatt tacgccgcgg gtgcaggaaa ggtggtgtat gtgggcaacc agctgcgtgg
    87481 ctacggtaat ctcatcatga ttaaacacag tgaagattac attacggctt acgcccataa
    87541 tgacacgatg ctggtaaata atgggcaaag cgtgaaggct gggcaaaaaa tcgccaccat
    87601 ggggagcacg gatgcggcat ctgttcgcct gcatttccag attcgttacc gtgcaacggc
    87661 aattgatccg ctacgttact tgccgccgca gggcagcaag ccaaaatgct gatggcgaat
    87721 taatcagcag tcagcagcgc ggcacttgct aaggagagcg taaggtttat aatgccttac
    87781 gcatctcgaa gcgggcgtag ttcaatggta gaacgagagc ttcccaagct ctatacgagg
    87841 gttcgattcc cttcgcccgc tccaatttat cttcacctca tcaatctgcc atttttgctg
    87901 tatctgattt tctatattat cttaattcaa tcgcttcaga cttttcgcaa tcgccgtaac
    87961 acttccccat ttttttgtaa ataaaaagcg tttac
//
LOCUS       DAZFFG010000007        80723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFFG010000007 DAZFFG010000000
VERSION     DAZFFG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.007"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..162
                     /gene="yhfL"
                     /locus_tag="ACHJ7J_000670"
     CDS             <1..162
                     /gene="yhfL"
                     /locus_tag="ACHJ7J_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO4998945.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
     gene            458..1795
                     /gene="frlA"
                     /locus_tag="ACHJ7J_000671"
     CDS             458..1795
                     /gene="frlA"
                     /locus_tag="ACHJ7J_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO4998946.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPSFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1816..2838
                     /gene="frlB"
                     /locus_tag="ACHJ7J_000672"
     CDS             1816..2838
                     /gene="frlB"
                     /locus_tag="ACHJ7J_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO4998947.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGEQASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2889..3716
                     /gene="frlC"
                     /locus_tag="ACHJ7J_000673"
     CDS             2889..3716
                     /gene="frlC"
                     /locus_tag="ACHJ7J_000673"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO4998948.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            3716..4501
                     /gene="frlD"
                     /locus_tag="ACHJ7J_000674"
     CDS             3716..4501
                     /gene="frlD"
                     /locus_tag="ACHJ7J_000674"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO4998949.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEGAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPELVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4601..5332
                     /gene="frlR"
                     /locus_tag="ACHJ7J_000675"
                     /gene_synonym="yhfR"
     CDS             4601..5332
                     /gene="frlR"
                     /locus_tag="ACHJ7J_000675"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO4998950.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5463..6467)
                     /gene="trpS"
                     /locus_tag="ACHJ7J_000676"
     CDS             complement(5463..6467)
                     /gene="trpS"
                     /locus_tag="ACHJ7J_000676"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO4998951.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(6460..7218)
                     /gene="gph"
                     /locus_tag="ACHJ7J_000677"
     CDS             complement(6460..7218)
                     /gene="gph"
                     /locus_tag="ACHJ7J_000677"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO4998952.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(7211..7888)
                     /gene="rpe"
                     /locus_tag="ACHJ7J_000678"
     CDS             complement(7211..7888)
                     /gene="rpe"
                     /locus_tag="ACHJ7J_000678"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO4998953.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(7906..8742)
                     /gene="dam"
                     /locus_tag="ACHJ7J_000679"
     CDS             complement(7906..8742)
                     /gene="dam"
                     /locus_tag="ACHJ7J_000679"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4998954.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(8849..10135)
                     /gene="damX"
                     /locus_tag="ACHJ7J_000680"
     CDS             complement(8849..10135)
                     /gene="damX"
                     /locus_tag="ACHJ7J_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO4998955.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(10227..11315)
                     /gene="aroB"
                     /locus_tag="ACHJ7J_000681"
     CDS             complement(10227..11315)
                     /gene="aroB"
                     /locus_tag="ACHJ7J_000681"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO4998956.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(11372..11893)
                     /gene="aroK"
                     /locus_tag="ACHJ7J_000682"
     CDS             complement(11372..11893)
                     /gene="aroK"
                     /locus_tag="ACHJ7J_000682"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO4998957.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(12294..13532)
                     /gene="hofQ"
                     /locus_tag="ACHJ7J_000683"
     CDS             complement(12294..13532)
                     /gene="hofQ"
                     /locus_tag="ACHJ7J_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO4998958.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(13444..13848)
                     /gene="hofP"
                     /locus_tag="ACHJ7J_000684"
     CDS             complement(13444..13848)
                     /gene="hofP"
                     /locus_tag="ACHJ7J_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO4998959.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPRWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(13838..14278)
                     /gene="hofO"
                     /locus_tag="ACHJ7J_000685"
     CDS             complement(13838..14278)
                     /gene="hofO"
                     /locus_tag="ACHJ7J_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO4998960.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRVSHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(14262..14801)
                     /gene="hofN"
                     /locus_tag="ACHJ7J_000686"
     CDS             complement(14262..14801)
                     /gene="hofN"
                     /locus_tag="ACHJ7J_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO4998961.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(14801..15580)
                     /gene="hofM"
                     /locus_tag="ACHJ7J_000687"
     CDS             complement(14801..15580)
                     /gene="hofM"
                     /locus_tag="ACHJ7J_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO4998962.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            15700..18252
                     /gene="mrcA"
                     /locus_tag="ACHJ7J_000688"
     CDS             15700..18252
                     /gene="mrcA"
                     /locus_tag="ACHJ7J_000688"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO4998963.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(18417..18977)
                     /gene="nudE"
                     /locus_tag="ACHJ7J_000689"
     CDS             complement(18417..18977)
                     /gene="nudE"
                     /locus_tag="ACHJ7J_000689"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO4998964.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            19297..21432
                     /gene="igaA"
                     /locus_tag="ACHJ7J_000690"
     CDS             19297..21432
                     /gene="igaA"
                     /locus_tag="ACHJ7J_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO4998965.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYTLQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21497..22165
                     /gene="yrfG"
                     /locus_tag="ACHJ7J_000691"
     CDS             21497..22165
                     /gene="yrfG"
                     /locus_tag="ACHJ7J_000691"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO4998966.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            22176..22577
                     /gene="hslR"
                     /locus_tag="ACHJ7J_000692"
     CDS             22176..22577
                     /gene="hslR"
                     /locus_tag="ACHJ7J_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO4998967.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            22602..23480
                     /gene="hslO"
                     /locus_tag="ACHJ7J_000693"
     CDS             22602..23480
                     /gene="hslO"
                     /locus_tag="ACHJ7J_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO4998968.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23543..25267)
                     /gene="yhgE"
                     /locus_tag="ACHJ7J_000694"
     CDS             complement(23543..25267)
                     /gene="yhgE"
                     /locus_tag="ACHJ7J_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO4998969.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYSYFYQSVWHNILILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            25646..27268
                     /gene="pckA"
                     /locus_tag="ACHJ7J_000695"
     CDS             25646..27268
                     /gene="pckA"
                     /locus_tag="ACHJ7J_000695"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO4998970.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(27344..28696)
                     /gene="envZ"
                     /locus_tag="ACHJ7J_000696"
     CDS             complement(27344..28696)
                     /gene="envZ"
                     /locus_tag="ACHJ7J_000696"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO4998971.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(28693..29412)
                     /gene="ompR"
                     /locus_tag="ACHJ7J_000697"
     CDS             complement(28693..29412)
                     /gene="ompR"
                     /locus_tag="ACHJ7J_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO4998972.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            29640..30116
                     /gene="greB"
                     /locus_tag="ACHJ7J_000698"
     CDS             29640..30116
                     /gene="greB"
                     /locus_tag="ACHJ7J_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO4998973.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            30213..32534
                     /gene="yhgF"
                     /locus_tag="ACHJ7J_000699"
     CDS             30213..32534
                     /gene="yhgF"
                     /locus_tag="ACHJ7J_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO4998974.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            32991..33218
                     /gene="feoA"
                     /locus_tag="ACHJ7J_000700"
     CDS             32991..33218
                     /gene="feoA"
                     /locus_tag="ACHJ7J_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO4998975.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            33235..35556
                     /gene="feoB"
                     /locus_tag="ACHJ7J_000701"
     CDS             33235..35556
                     /gene="feoB"
                     /locus_tag="ACHJ7J_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO4998976.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            35556..35792
                     /gene="feoC"
                     /locus_tag="ACHJ7J_000702"
     CDS             35556..35792
                     /gene="feoC"
                     /locus_tag="ACHJ7J_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO4998977.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            35995..36873
                     /gene="rpnA"
                     /locus_tag="ACHJ7J_000703"
     CDS             35995..36873
                     /gene="rpnA"
                     /locus_tag="ACHJ7J_000703"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO4998978.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(36900..37670)
                     /gene="bioH"
                     /locus_tag="ACHJ7J_000704"
     CDS             complement(36900..37670)
                     /gene="bioH"
                     /locus_tag="ACHJ7J_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO4998979.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSAREEWPGIKPEVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHLLVALKQRV"
     gene            37708..38391
                     /gene="gntX"
                     /locus_tag="ACHJ7J_000705"
     CDS             37708..38391
                     /gene="gntX"
                     /locus_tag="ACHJ7J_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO4998980.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            38450..39025
                     /gene="nfuA"
                     /locus_tag="ACHJ7J_000706"
     CDS             38450..39025
                     /gene="nfuA"
                     /locus_tag="ACHJ7J_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO4998981.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            39386..40702
                     /gene="gntT"
                     /locus_tag="ACHJ7J_000707"
     CDS             39386..40702
                     /gene="gntT"
                     /locus_tag="ACHJ7J_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4998982.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(40747..42831)
                     /gene="malQ"
                     /locus_tag="ACHJ7J_000708"
     CDS             complement(40747..42831)
                     /gene="malQ"
                     /locus_tag="ACHJ7J_000708"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO4998983.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQVWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDNE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSCGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(42841..45234)
                     /gene="malP"
                     /locus_tag="ACHJ7J_000709"
     CDS             complement(42841..45234)
                     /gene="malP"
                     /locus_tag="ACHJ7J_000709"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO4998984.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHQAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            45846..48551
                     /gene="malT"
                     /locus_tag="ACHJ7J_000710"
     CDS             45846..48551
                     /gene="malT"
                     /locus_tag="ACHJ7J_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO4998985.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(48594..49610)
                     /gene="rtcA"
                     /locus_tag="ACHJ7J_000711"
     CDS             complement(48594..49610)
                     /gene="rtcA"
                     /locus_tag="ACHJ7J_000711"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO4998986.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFKTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(49614..50840)
                     /gene="rtcB"
                     /locus_tag="ACHJ7J_000712"
     CDS             complement(49614..50840)
                     /gene="rtcB"
                     /locus_tag="ACHJ7J_000712"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO4998987.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEK
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            51027..52625
                     /gene="rtcR"
                     /locus_tag="ACHJ7J_000713"
     CDS             51027..52625
                     /gene="rtcR"
                     /locus_tag="ACHJ7J_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO4998988.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIVSVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(52607..53365)
                     /locus_tag="ACHJ7J_000714"
     CDS             complement(52607..53365)
                     /locus_tag="ACHJ7J_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO4998989.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(53382..54212)
                     /gene="glpG"
                     /locus_tag="ACHJ7J_000715"
     CDS             complement(53382..54212)
                     /gene="glpG"
                     /locus_tag="ACHJ7J_000715"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO4998990.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(54257..54583)
                     /gene="glpE"
                     /locus_tag="ACHJ7J_000716"
     CDS             complement(54257..54583)
                     /gene="glpE"
                     /locus_tag="ACHJ7J_000716"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO4998991.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            54773..56278
                     /gene="glpD"
                     /locus_tag="ACHJ7J_000717"
     CDS             54773..56278
                     /gene="glpD"
                     /locus_tag="ACHJ7J_000717"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO4998992.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEITRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYRWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(56493..57095)
                     /locus_tag="ACHJ7J_000718"
     CDS             complement(56493..57095)
                     /locus_tag="ACHJ7J_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4998993.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACIITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFVRRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(57095..58087)
                     /locus_tag="ACHJ7J_000719"
     CDS             complement(57095..58087)
                     /locus_tag="ACHJ7J_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4998994.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINDVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFLSPQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(58113..59621)
                     /locus_tag="ACHJ7J_000720"
     CDS             complement(58113..59621)
                     /locus_tag="ACHJ7J_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4998995.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWDYLE
                     NSRTPEAYNKIVKGDADIIFVAQPSGGQKKRAKESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTFGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENKTSETQKLVEWFLTPQGQSLVEDVGYVPLYPTME"
     gene            complement(59747..62194)
                     /gene="glgP"
                     /locus_tag="ACHJ7J_000721"
     CDS             complement(59747..62194)
                     /gene="glgP"
                     /locus_tag="ACHJ7J_000721"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO4998996.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(62213..63646)
                     /gene="glgA"
                     /locus_tag="ACHJ7J_000722"
     CDS             complement(62213..63646)
                     /gene="glgA"
                     /locus_tag="ACHJ7J_000722"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO4998997.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(63646..64941)
                     /gene="glgC"
                     /locus_tag="ACHJ7J_000723"
     CDS             complement(63646..64941)
                     /gene="glgC"
                     /locus_tag="ACHJ7J_000723"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO4998998.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITDAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(64959..66932)
                     /gene="glgX"
                     /locus_tag="ACHJ7J_000724"
     CDS             complement(64959..66932)
                     /gene="glgX"
                     /locus_tag="ACHJ7J_000724"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO4998999.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(66929..69115)
                     /gene="glgB"
                     /locus_tag="ACHJ7J_000725"
     CDS             complement(66929..69115)
                     /gene="glgB"
                     /locus_tag="ACHJ7J_000725"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO4999000.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGIQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGNWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(69388..70491)
                     /gene="asd"
                     /locus_tag="ACHJ7J_000726"
     CDS             complement(69388..70491)
                     /gene="asd"
                     /locus_tag="ACHJ7J_000726"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4999001.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            70683..71276
                     /gene="yhgN"
                     /locus_tag="ACHJ7J_000727"
     CDS             70683..71276
                     /gene="yhgN"
                     /locus_tag="ACHJ7J_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO4999002.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(71322..72617)
                     /locus_tag="ACHJ7J_000728"
     CDS             complement(71322..72617)
                     /locus_tag="ACHJ7J_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO4999003.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHKWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(72524..74620)
                     /locus_tag="ACHJ7J_000729"
     CDS             complement(72524..74620)
                     /locus_tag="ACHJ7J_000729"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO4999004.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPITDHIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLSLNLNKQVAAGAFTQYGFKGNLPAILQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(74760..76100)
                     /gene="gntU"
                     /locus_tag="ACHJ7J_000730"
     CDS             complement(74760..76100)
                     /gene="gntU"
                     /locus_tag="ACHJ7J_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4999005.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(76104..76631)
                     /gene="gntK"
                     /locus_tag="ACHJ7J_000731"
     CDS             complement(76104..76631)
                     /gene="gntK"
                     /locus_tag="ACHJ7J_000731"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO4999006.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(76770..77765)
                     /gene="gntR"
                     /locus_tag="ACHJ7J_000732"
     CDS             complement(76770..77765)
                     /gene="gntR"
                     /locus_tag="ACHJ7J_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO4999007.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(77989..78684)
                     /gene="yhhW"
                     /locus_tag="ACHJ7J_000733"
     CDS             complement(77989..78684)
                     /gene="yhhW"
                     /locus_tag="ACHJ7J_000733"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO4999008.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(78807..79844)
                     /gene="yhhX"
                     /locus_tag="ACHJ7J_000734"
     CDS             complement(78807..79844)
                     /gene="yhhX"
                     /locus_tag="ACHJ7J_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4999009.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            80179..80667
                     /gene="yhhY"
                     /locus_tag="ACHJ7J_000735"
     CDS             80179..80667
                     /gene="yhhY"
                     /locus_tag="ACHJ7J_000735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4999010.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        19445 a        21958 c        20283 g        19037 t
ORIGIN      
        1 aaatttatta aagttgcact ggtaggtgcg gtactggcta cgttaactgc atgtactggt
       61 catattgaaa accgtgataa gaactgctct tacgactacc tgctgcaccc agcaatttct
      121 atttctaaaa tcattggcgg ttgcggtcct actgcacagt aagtctctga cagaaatcag
      181 ctaacaccgg tttaattgcc cgacgccctc tggattttcc gagggcgtat tggtttctat
      241 atccttcttg ctccccaccc gtaaaaatat tttcacttta atttcagttg gataaccaca
      301 ttcatcagcc acagcgcgat cctgtgcgaa attatgtgat cttcctccac attacataac
      361 atcatatgtt gttatattca tcatgcattg tcatgttacc ttttaaatga ctgcaaactc
      421 tcccctacaa cgtgttggac aagaaaaagg tatcgctatg ggaagccagg aactccaacg
      481 caagctcgga ttttgggccg ttcttgcaat cgccgtcggg acaaccgtcg gctccggtat
      541 ttttgtatct gtgggtgaag tggcaaaagc ggcgggcacg ccgtggctga cggtgctcgc
      601 atttgtcatc ggcgggttaa ttgtgatccc gcaaatgtgc gtctatgcgg aactatccac
      661 cgcttatccg gaaaatggcg cagattatgt ttatctgaaa aatgccggaa gccgaccgct
      721 ggctttcctc tccggctggg ccagcttctg ggccaacgat gcgccgtcat tgtcgattat
      781 ggcgctggcg atagtcagca atcttggctt tttaacgcct atcgatcctc tgctcggtaa
      841 atttatcgcc gccggattaa ttatcgcctt tatgctgctg cacctgcgct ccgttgaagg
      901 cggcgcagcg tttcagacgc taattaccat cgccaaaatt atcccgttca ctatcgtcat
      961 tggtcttggg atcttctggt ttaaagcgga gaattttgcc gcccctacca ccactgcgat
     1021 tggcgcaacg ggcagcttta tggcgctact ggcgggaatc tccgccacca gttggtcgta
     1081 caccggcatg gcctctatct gttatatgac cggcgaaatt aaaaaccccg gtaaaactat
     1141 gccacgagcg ctaattggtt cctgtctgct ggttctggtg ctctacaccc tgctggcgtt
     1201 ggtaatttct ggcctgatgc ccttcgacaa actcgccaat tctgaaacgc cgatttccga
     1261 cgccctgacc tggatcccgg cactcggcag caccgccggg atcttcgtgg ctatcacggc
     1321 gatgatcgtc attctcggat cgctttccag ctgcgtgatg taccagccgc ggctggaata
     1381 cgcgatggcg aaagacaatc tgttcttcaa atgcttcggc catgtgcatc caaaatacaa
     1441 cacgccggat gtctccatca tcctgcaagg ggcgctgggg atcttcttca tcttcgtttc
     1501 cgatctcacc agcctgctgg gttatttcac cctggtgatg tgtttcaaaa ataccctcac
     1561 cttcggctcc atcatctggt gtcgtaaacg cgacgattac aaaccgctgt ggcgtactcc
     1621 gtctttcggg ctgatgacca ccctcgccat tgcatcaagc ctcattctgg tcgcctcaac
     1681 ctttgtctgg gcaccgattc ccggccttat ctgcgccgtc atcgttattg ccactggtct
     1741 gcctgcttac gccttctggg cgaagcgtag ccgccagctc aacgctttgt cgtaatgtta
     1801 tctggagaaa ataaaatgtt ggatattgat aaaagcaccg tggactttct ggtcaccgaa
     1861 aatatggttc aggaagtgga aaaagttctc agccatgacg ttccgctggt gcacaccatc
     1921 gtggaagaga tggtgaagcg cgacatcgat cgtatttatt tcgttgcctg cggatcgcca
     1981 ctcaacgcgg cgcaaacggc gaaacatctg gcagatcgct tttccgatct tcaggtttac
     2041 gccatttccg gctgggagtt ctgcgataac accccgtatc gcctcgacga tcgttgcgcg
     2101 gtgattggcg tttctgacta cggtaaaacc gaagaggtga tcaaagcgct ggaactgggt
     2161 cgggcctgcg gcgcgcttac tgcggcattc accaaacgcg cggacagccc gattacctcg
     2221 gcggcggaat ttagcattga ttatcaggcc gactgtatct gggaaattca cctgctgctc
     2281 tgctacagcg tggtgctgga gatgatcacc cgcctcgcgc caaacgcgga aatcggcaag
     2341 atcaaaaacg atctcaaaca gctgccgaat gcgctcggtt atctggtacg cacctgggaa
     2401 gagaaaggtc gccagctcgg tgaacaggcc agtcagtggc cgatgattta cacggttgcc
     2461 gcaggcccgc tgcgcccgct gggttacaaa gaaggcatcg tgacgctgat ggaatttacc
     2521 tggacgcacg gctgtgtgat tgagagcggt gagttccgtc acggcccgct ggagattgtc
     2581 gaaccgggcg ttccgttcct gtttctgctc ggcaatgacg aaagtcgtca caccaccgaa
     2641 cgcgccatta actttgttaa acagcgtact gacaacgtga tcgtcatcga ttacgccgaa
     2701 atttcgcaag ggctgcaccc gtggctggca ccgttcctga tgttcgtgcc aatggagtgg
     2761 ctctgctact acctgtctat ttacaaagat cacaacccgg atgaacgccg ctattacggt
     2821 ggtctggtgg aatattaatc ctctctcccg gcccggcaac gggccggatt tacgcaagga
     2881 gttacccgat gaaaacaggt atgtttacct gcggccacca gcggctgcct attgaacacg
     2941 cattccgtga tgcaagcgag ctgggttacg atggcatcga aatttggggc ggccgcccgc
     3001 acgcgttcgc gccggactta aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga
     3061 cgtatcagat gccgattatc ggttacacgc cagaaaccaa cggctatccg tataacatga
     3121 tgctgggcga tgaacatatg cgtcgtgaaa gcctggacat gatcaagctg gcgatagata
     3181 tggcaaaaga gatgaacgcg ggttatacgc tgatttctgc ggcccacgcg ggctatctca
     3241 cgccgcctaa cgttatctgg gggcggctgg cagagaactt aagcgaactg tgtgagtacg
     3301 cggaaaacat cggcatggat ctgatcctcg aaccgttaac gccgtatgaa tcgaacgtcg
     3361 tgtgtaatgc taatgatgtg cttcatgcgc tggcgctggt gccttcgccg cgcctgttca
     3421 gcatggtcga catttgcgcg ccgtatgtcc aggcggaacc ggtgatgagt tatttcgaca
     3481 aactgggcga taaattacgt catctgcata ttgtcgacag cgacggggcc agcgacacgc
     3541 attacattcc tggagaaggc aaaatgccgc tgcgggaact gatgcgcgat attattgatc
     3601 ggggctatga gggatactgc acagtggaac tggtaacgat gtatatgaac gagcccagac
     3661 tttacgcccg ccaggcgctg gaacgctttc gcgcgctgct gccggaggat gagtgatgaa
     3721 aaccctggcg acaatcggcg ataactgcgt cgatatctac ccgcaactga ataaagcgtt
     3781 ttctggcggt aatgcggtga acgtggcggt gtactgcact cgctacggta taaagccggg
     3841 atgcattacc tgggtgggtg acgatgacta cggcacaaag ctgaagcagg atctcgcccg
     3901 catgggcgtc gatatcagcc atgtccatac gaaacacggc gtaaccgcac aaactcaggt
     3961 ggaactgcac gacaacgatc gcgtttttgg cgactacacc gaaggcgtga tggccgactt
     4021 tgccctgagc gaagaagatt acgcctggct ggcgcagtat gacattgtgc acgcggcaat
     4081 ctggggacat gcggaaggcg ctttcccgca gctgcacgca gcgggcaaac ttaccgcttt
     4141 cgacttctcc gacaagtggg acagcccgct ctggcagaca ctggtgccgc atctcgattt
     4201 tgcctttgcc tctgcaccgc aagaagacga agcgctgcgt ctgaagatga aagcgattgt
     4261 tgcccgtggc gcaggaacag tgattgtcac gctgggcgaa aacggcagca ttgcctggga
     4321 tggcgcgcag ttctggcgtc aggctcctga actggtgacg gttatcgaca ccatgggtgc
     4381 cggagattcg ttcattgccg gattcctttg cggctggtct gcggggatga cattaccgca
     4441 ggcgatggcg cagggaacgg cgtgcgcggc gaaaaccatt cagtaccacg gtgcctggta
     4501 ggtataacgt tggcgtgagc atcttcacgc caacgtgctg ttacttgccg gaaaacgacc
     4561 ctataatccg agtaattcat tctttatttc agggtcgatt atgtcagcta cggaccgcta
     4621 ctctcatcaa ctcctctacg ctaccgtccg ccagcggctg ctggatgata tcgcgcaggg
     4681 ggtttaccag gccgggcaac agatccctac cgaaaacgag ctttgtacac aatataacgt
     4741 cagccgcatt accattcgca aagccatcag cgacttagtg gcagacggcg tactgatccg
     4801 ctggcagggc aaaggcacct ttgtacaaag ccagaaggtc gaaaacgccc tgctgaccgt
     4861 cagtggtttt accgattttg gcgtctcaca aggcaaggcg acgaaagaga aagtgatcga
     4921 gcaggaacgg gtcagcgccg cgccgttttg cgaaaagctg aacatccctg gcaacagcga
     4981 agtgttccat ctttgccggg tgatgtatct cgataaagag ccgctgttta ttgatagttc
     5041 ctggatcccg ctgtcgcgtt atcctgactt tgatgagatt tacgtcgaag gaagctccac
     5101 ctatcagtta tttcaggagc gttttgacac gcgagtggtc agcgacaaaa agaccatcga
     5161 tatctttgcc gccacccgcc cgcaggcgaa atggctgaaa tgcgaactgg gcgaaccgtt
     5221 gtttcgcatc agcaaaatcg cctttgatca gaatgacaaa ccggtgcacg tctccgaact
     5281 cttctgccgc gccaaccgca tcaccttaac gattgataat aaaagacatt aaccgtaggc
     5341 cggataagat gcaccagcat cgcatccggc gatgccggcg cactgaattt tacatcccgc
     5401 acgttcccct caccctaacc ctctccccaa aggggcgagg ggaccgtccg agcgttttga
     5461 tattacggct tcgccacaaa accaatcgct tcatacaccg cttttagcgt acgggaagcg
     5521 tgcgcgctgg ctttttctgc gccgtctttc atcacctgtt gcaggaaggc ttcatcgttg
     5581 cggaaacggt gatagcgttc ctgcaattca gtcagcatac cggaaacggc atcagccact
     5641 tcacctttca gatgaccata catcttgcct tcgaactgtt tttccagttc tgggatgctc
     5701 tggcccgtta ccgcagaaag gatatccagc aggttggaaa cgcccgcttt gttctgcaca
     5761 tcatagcgaa ctaccggcgg ctcgtcggag tcggtgaccg cgcgtttgat tttcttcact
     5821 accgatttcg gatcttccag caggccaata acgttattgc ggttatcgtc agacttggac
     5881 atcttcttgg tcggctccag cagcgacatt acgcgcgcgc cggatttcgg aataaacggc
     5941 tccggcacct taaagatatc gccgtacagc gcgttgaaac gctgggcaat atcgcggctc
     6001 agttcgaggt gctgtttctg gtcttcaccc accggtacca gattagtttg atacagcagg
     6061 atgtccgctg ccatcagcac cggatagtca aacagaccag cgttgatgtt ctcggcataa
     6121 cgcgcagatt tatctttaaa ctgcgtcatg cggctcagtt cgccgaagta ggtatagcag
     6181 ttcagtgccc agcctaactg cgcatgttct ggtacgtggg actgaacaaa aatggtgctt
     6241 ttctcaggat cgataccaca agccagatac aaggccagcg tatccagcgt cgctttacgc
     6301 agcttctgtg catcctggcg cacggtgatc gcgtgttggt caacgataca gtaaatgcaa
     6361 tggtagtcat cctgcatgtt tacccactga cgcagcgcac ccatgtagtt accaatggtc
     6421 aattcacctg agggctgtgc gccactaaaa acgatgggct tagtcatttt tcgattcctg
     6481 attttcgcta tgcggaagcc ctaatgcggg cagaaggtca tttatagact gataaattac
     6541 atcaggctgg ctgagatcga tagcctcgcc gtagttatat ccgtaggtta agccaactga
     6601 tgggcaacct gccgcttttg ctgcctgaat atcattgcgt gagtcgccaa caaacagcat
     6661 ctgttgtggg gcaattccca tccgctcagc caccagtaac agcgggtccg gatgcggttt
     6721 tttgttttgc acatcatcac caccaatcac cacgctgaag tatttggcga tatctaaggc
     6781 ttcgagcagc ggcgcgacga acggcgtcgg tttgttggtg accaggccta gcggcaggcc
     6841 tttagcctgc aacgcgccca acgtatcggc aacgtgcggg aacaaaaacg tcccctcttc
     6901 ggcaacctcg ccataatagc gatcgaacag tttacgcaga atacgtacct gttcttctgc
     6961 cggaatgtcg tcatcaacgg gcggtttacc cattgtttta cgcagagtcg cacgttcctg
     7021 acgcgcccag gtcaatgcgc gctccatcag aacatctgcg ccgttaccaa tccaggtaat
     7081 aacgcgttct tcacctgcga cgggtaactc cagcgcatac agcgccatat ctaccgcagc
     7141 agcaagaccg ggagcactgt ccaccagcgt accgtcgaga tcaaaagcga cgccgcgaat
     7201 atcttcaaac ttactcatga cttacctttg ccagttcact gcgcatttca tcaatgactt
     7261 ttttgtagtc tggctggtcg aagattgccg aaccggcaac gaacatatcc gcgcccgccg
     7321 cggcgatttc gccaatgttg ttcaccttca cgcctccgtc cacttccaga cgaatgtcaa
     7381 agccagactc gtcgatacga cgacgtactt cgcgcagttt atccagtgtt tgaggaatga
     7441 aagactgacc gccgaaacca ggattgacgg acatcagcag gatcacatcc agcttatcca
     7501 tcacgtaatc cagatagctc agaggtgtcg ccgggttaaa taccagaccc gctttacagc
     7561 cattttcttt aatcagttgc agcgtgcggt caacatgctc ggaggcttct ggatgaaagg
     7621 taatgatgct ggcaccagcg gcagcgaaat caggcacaat gcgatcgaca ggtttcacca
     7681 tcaggtgtac gtcgataggg gcggtaatgc cgtagttacg caaggatttc agcaccattg
     7741 gcccaatcgt cagattggga acatagtggt tatccatgac gtcaaaatgc acgacatcag
     7801 cgccagctgc cagggctttt gcggtatctt cacccaggcg ggcaaaatca gccgacagaa
     7861 ttgagggggc aatcaaatac tgtttcatcc gcttctcctt gagaattatt ttttcgcggg
     7921 tgaaacgact cctggtttgt acaaagccag cagttcgtcc acctttttac gtgtgccgcc
     7981 gttgctgctt atactgcgtc gaactttgac gacatgcaat tttgcgcgct gataccactc
     8041 acgcgttaac atcgtatcgt gattggagat cagcacagga atatggcgct caaccagacc
     8101 ttcggcgatc tccgccagat gcgcttgttg ttcaagcgta aaactgtttg tgtgatacgc
     8161 cgtaaagttg gcggtcgcag acagcggtgc ataaggcgga tcgcaataga cgacagatgc
     8221 atcatctgcg cgcgccatgc tatcggcgta agactcacaa tagaaaaagg cattctgcgc
     8281 tttttcagcg aagtgataca actctgcttc cgggaaatag ggttttttgt agcggccaaa
     8341 cggcacatta aactcaccgc gcagattgta acgacacagg ccgttgtaac cgtagcggtt
     8401 caaatataaa aacagtaccg cccgacggaa cggatcctgg cttttgttga actcttcgcg
     8461 gaactgatag taaacctcgg cgcaatttgt ttcgggaaca aacagctcgc gtgcggcctg
     8521 tacgtactca tcagtacgca tcttcacaat gttatagaga ctgatcaggt cgctattgat
     8581 atcggcaagg atataacgag aaaagtcggt gttgagaaac accgacccgg cacctacaaa
     8641 aggctcaacc agacattcgc ccttgggcaa atgccgttta atatcatcaa gcaggggata
     8701 cttgccccct gcccacttca aaaaagcgcg atttttcttc atgctgacta actaattaca
     8761 ccttctccgg ctgtggagaa agctccgaca gcatccagcg cttcaggcaa ctcccgcaac
     8821 gataaaccat tgcgggagat aaccttgatt acttcagatc ggcctgtacc tgacgcagcg
     8881 gtttcgccca cgggtttttg gcctgaacat ctgctggcaa tgtagatacc gcttttttcg
     8941 cctcttcttt cgaagcgtac acgccagaaa ccaggacata ccacggctga ccattacgcg
     9001 tcgtttcata gacaacgtag tttttcagat tctctttctt cgcccaaccg ttcaggttgt
     9061 cgtagttaga ggaactgctc agctgcagag tgtaatggct ggacggtgcc gatttcaacg
     9121 aaccaacatt acctgcggtc ttccctccag cggctggtgt tgccgtggtt tgcgccgggg
     9181 atgccgtctg tactggagcc gtagtcgccg tctcttttgg tgctggcgta gaagtcgcag
     9241 ccggtgcctt cgtgctcgcc acaggagcag ctggttcagt acgcttcggc gtctgtgcta
     9301 ccggcttcgg ttccgttttc acggttgctt gcggtttttt cggttcaatc accgcctgct
     9361 gacgagcagg acgcgtagtg gccggacgtt cagcagtttg cgttttcgcc gtgtcacgcg
     9421 atgcattgcc attgcgtact ggcgcgacag ttgctggttc agtcggcaat gtagaattga
     9481 ccgccacatt gttcaactgt tgctgatttt gtggctgggt cagcgcattg ttcaggtcac
     9541 cctgcacttc aacacgttgt tgaccatccg ttgccgccgg ggtttgccct tgagtcggcg
     9601 tagaagagat cggcggcaga gaaacatcct gctgagtatt ttccgcagac gtggttcccg
     9661 gcgctggctg cacgccattg gcctgatcgg tcgcattgcc agcaagatca atactcttct
     9721 cgccagacgc ggtttgatcg ctggaagagg tcgagggggc ttttagcgca gaaccgatac
     9781 cgatgatcaa cagcagtaga accagaatgc cgacgcccat catcatatac tgacgagaag
     9841 cgggtttact ggctgctttt ttgcgcttac gcggacgacg ctctacgcgc tcttcatcca
     9901 cggtttcatc ttcggattcg tcaatttctt cttcgatttc cggttcctca ttgcgctctt
     9961 tttgcgcacg agtcggacgg cgatcgtcag tgtcatcaag ttcaatatca tcaaaattga
    10021 tctgcggttc gccacgttca gtacgctcag aacgttcaga agattgacga gaacgaccag
    10081 tacgacgatc gctgggatcg ggtttcagct cgtcttctgg tttgaattca tccatttaac
    10141 accccactaa aaggttaatg cttaccacgt tgcaattaac ctgaagctaa tagaccgctt
    10201 gataagcggc ctgacctttc ttgttgttac gctgattgac aatcggcaat ggcgttaaga
    10261 acaagctcgt gcgaaacgcc gctgcgaact tcactcttac caattgccaa cggaagaatt
    10321 aagcgcatct ctcccgcaag gactttcttg tcacgcagca tatgcggtaa atacgcctgc
    10381 gcggacattt cgcgcggccc attgaccggt aacccagccc gcttgagcag ggttataata
    10441 cgctgcgttt cggcagaact aaactgcccg agacgttccg acgtccgcgc cgccatcacc
    10501 atacccgcag cgaccgcttc accatgtaac caattgccat accccatttc agcttcaatg
    10561 gcatgaccaa aggtgtgtcc cagattcagt aaagcacgta acccggtttc gcgctcgtcg
    10621 gcggcgacaa cttctgcctt cagttcacaa caacggcgaa tacagtacgc cattgccgga
    10681 ccgtccagac gcaacaacgc atccagattc tcttccagcc agttaaaaaa cgcaccgtca
    10741 agaataatgc cgtatttgat gacttctgcc agccccgacg ctaactcacg cgggggaagc
    10801 gttttcagac agtcgagatc caccaccact gaagcaggct ggtagaacgc gccaatcatg
    10861 tttttaccga ggggatggtt gaccgcagtt ttgccgccaa cggaggaatc gacctgcgac
    10921 agtaacgtcg tcgggacttg aatgaaacgt acaccgcgct gataactcgc cgccgcgaag
    10981 ccggtcagat cgcccactac gccgccgcca agcgccacca gcgtagtatc gcgaccatgc
    11041 ggcttttgta acaacgccgt aaagacggta tcgagtacag ccaggctttt atactgctcg
    11101 ccgtcaggaa ggataacgct atcgacgtta acacccgcct gttcaagtac gccgcggacc
    11161 ttatcgagat acagaggagc cagggtttcg ttggtgacca acatgacctg ctcgcccgat
    11221 ttcagcggta agaatgaagc tggttcatta aacaaaccag atgcgatggt aattgggtaa
    11281 ctacgttccc cgagagtaac gacaatcctc tccataacgc gacatccacc ttaattactg
    11341 tacccgcaga cgagtgtata taaagccaga attagttgct ttccagcatg tgaataatct
    11401 ggtttgcaac cactttagcg ctttgatcat cagtacgaat ggtcacgtcg gcaatctctt
    11461 catacagcgg attgcgttca ttggccaacg cttccagaac ttcacgtggc ggtgtttcaa
    11521 cgtgcagcaa cgggcgtttt ttatcacgct gcgtgcgtgc aagttgcttt tcgatggtcg
    11581 tttcaagata aacgacaacg ccacgagcgg aaagacggtt acgcgtttca cgggatttca
    11641 cagagccgcc gccagtagcc agcacaatac cctgtttctc ggtcaactca ttgatgacct
    11701 tttcttcgcg atcgcggaag ccttcttcgc cttctaaatc gaaaacccag cccacatcag
    11761 ctccggttcg tttctcaatc tcttgatcgg aatcgtaaaa ttccatattg agttgttgag
    11821 ctaactggcg cccaatagtg ctttttccgg cacccatagg cccaaccaga aagatattgc
    11881 gtttctctgc cattttttcg gtactactaa gactattcgt taatgataaa cccgcttcgc
    11941 tcagagagcg ccgcaggaca tgaactgaaa cctcataaga tattgcgaga gtcagactga
    12001 aaattatctc aatactccag cgggtttggc aactgaataa atcacccatc cctctgcata
    12061 tctggtcgct gcaagcgcgc tgccttgcta ccaccgctct ggcgataaat caccgggtaa
    12121 gattagcgta aaaaagacag caaaatgccg cctgaatgat aaatcatcat catggaacgc
    12181 aggcagcgaa gagcaccaac tctcaaatcg gtactccttg tatgctaaat acctgcgcgc
    12241 gtcaaataga tgaaacacgt tcagcgtaaa aacattaccg cttttacggc tgtttactca
    12301 ctggaaacca gtcgtggcgt gataaacacc actaactcgc gtcgttcatc ttcttttccg
    12361 tcatgacgaa ataattgccc gaaccaggga atgtcgccaa gcaacggcac gctatcctga
    12421 cccgatttat ttttacgggt aaaaatgccg cccagcgcca acgtttctcc gcttttgacc
    12481 tcgacctgcg tttcgatctc ctgcttatca atcgccagca cttcgccatc ggcctgctgt
    12541 agcacctgcc ccggaacgtt ctggctgatg tgtaatttca gccggatgcg acctttttgt
    12601 aacaccgtgg gcgtgacctc catccccagg actgcctctt taaattccac cgacgtcgcg
    12661 ccactttccc cgctggaaac ctgatatgga atttcgctcc cctgtttaat gctggcaggc
    12721 tgaagatgtg aggccagcag acgcggacta gcgataatat ccagctgctg tttttgttcg
    12781 agcgcagaaa gctcaagatc cagcaaacgt ccattgatgc gcccaatgtt aaaaccgaca
    12841 tgcgttgtcg ccgtcgctac ggagaggtcg ctgccaagcg tggtgacttg cccaacgcca
    12901 ccagcttgtt gcgcatcggc cagcgtccat ttcacgccta actcacgcaa acttttttca
    12961 ttaatggtga caatatgcgc cgacagctca acctgcccga ccggcagatc catttgcgct
    13021 acccactgtt caagggcgct taacgccgtt ttgttatctc gtagcaaaag gcgattggtg
    13081 cgtttatcga cggtcatact ccctttggcg ctcagtagct tctccccagc tttcgccagc
    13141 tctccggcgt cggcgtattg cagagtaata ttgcgatttt ccagcggcag atttgcctgc
    13201 gcccgcgcct gctccgcttc ctgacgggcg atattgtcat tctgccaggc aatggaatgc
    13261 actgagagaa tgttgccctc ctggcgcgtt atcagtccgg cgcttttcac tacagtttgt
    13321 agtgcctgct tccagggaac atctgttaga tgtaacgaca ccgtaccgct gacgtctggc
    13381 gacaccacca ggttcaactt ctcctgttca gccagcgcct gcaacacctg agctaccgga
    13441 acgtcatcca ccatcagcgt cactttttgc ggctttgccg cctgtacgcc gggtatcagc
    13501 atcaacagta gtgcggctat ccattgcttc atttgtatct ccttgccgtt gccacaacca
    13561 tcgtggcggt tcgcagtttg tcccggtacc cagcgttagt acgtctggcg tcagctgtaa
    13621 aattgtccag ccgttttcca gcacatcgtt ttgctgcacc cgtcgccatt tcttttgccc
    13681 gtcttttatt acaccgatga tgcgctcgcc tcgccctacc atcccctgat agcgccactg
    13741 gctaagttcg ctaatccggc atagatcttc cggcggttta aaagggtcac gcataccggt
    13801 taaaaggcac aatgcaatac ctgccaacaa ccagcgttta accctcatgc ggcgtctcca
    13861 gttgtagcgt gaacaaaaga tcatcacctt ccacgcttaa tgaaaaacgg ctcacgctga
    13921 cgttgcgctc tgccagccgt gtaaatgccg acggcactgc ttcccacagc gttttcaacg
    13981 ccaactcacc tccctgcgcg gatggatgcc agaaaaccag ttgtgcgccg ggcaactgaa
    14041 aatccagtgg cgaaaagggc agcgtttttt cttcgctgaa gggagtggtg tcaaccaggc
    14101 gatacagtgt ggcccactgc tgatgatgac ttacccgtag tcgaattaat gcgtcgcgct
    14161 cttcatggtg tgtcgatgac agaaaaatga gcgtaactaa catcagcaac cagactgccc
    14221 agcaaagctg gcggagacgg ggggatgtgg cgaaccacca gtcaaagaac atgttcatcg
    14281 ctaaccttcc ttgttaactg atactcaaat tgccagcgtc cctgcgcatc ctgctgcgtg
    14341 gccccccgct gattgagatg aaaagatgca tcctggcgga gtgacgtttc tagtgcgttt
    14401 aacgcggtaa tgcttgttgt aagccccttg atctccagcg ttccctgctg ccagcttatc
    14461 gttgtcagcc aggcgtgctc gggtaaaagc gccgccagtg cttccagcgc agattgccag
    14521 tcgcgggtaa attgtcgctg gcgctgcctt tgcaaacgct gctcacgaat ttgttggcgc
    14581 tccagcaaac gtggtttcgt tatctgtaag ctgcgggcga gttgttgttc cccctgaagc
    14641 aaaacggcgt ctacgcgagc ttcggcgctg cttgtcagac gcagtattag cgttatcccg
    14701 acggccagca gcagaggcgc aacgaacatc agcaaccaga aacgaagaaa agcggtccgg
    14761 cgttgctgtc gccagggcaa aaaattaatt ggcgggttca tcagtacgcc ttcccaagcg
    14821 ccagccccag cgcgatggca aagtctccac ctggcggcgg taacggcggc tgacgaacag
    14881 aaacggcctc ccagggatca aatccgcctt cgccacatat cgcgacgctt tcgggatcaa
    14941 cggataacgc tgccgccagc tccttcgcgc tagtcatccc caccgccagt ttgcgccccc
    15001 agcgatagcg cgtcgcccac agccactgtt cgttatcacg ccaggccaga cattgctgat
    15061 gagaaggtaa aaaaggcagg aatcgctgta atgcactggc atccggggtg atcgcactaa
    15121 catgaacacg caacctttct gccagagtaa gcagcgttgc cagctctttg ctttgcgcgg
    15181 cagtcacgtt ataggcggga ctgagtgagt cttcgctgta atcgaagcgc agggagtccg
    15241 gatccatatc cagctcgcga gccatcgtcc cggacagcca ggccgtttgc tcccgctcac
    15301 cgagggacat cgacgggcgc ggaaatgacc gctgtaatgt gcgactggcg ggaaacgcta
    15361 acatgatgtg atggcgctgc ggcagttcgc gactccacgg taacaacgtt ttagccagcc
    15421 gctgcgcatc aacaatccgc ccatctttga taatgtcgtg cgccagcggc aaccgccacc
    15481 agcgttgcaa aaagcattct tttgcgcccc gcacgatagc aaccgctacc gcttcttgct
    15541 gttgtaaatg caaaccaatt tgccagatct taaatgccat tgtgatgatc tccttatcac
    15601 ccgtcactct gacgggtata tcaatgcgtc tggcttgcct ttatactacc gcgcgtttgt
    15661 ttataaactg cccaaatgaa actaaatggg aaatttccag tgaagttcgt aaagtatttt
    15721 ttgatccttg cagtctgttg cattctgctg ggagcaggct cgatttatgg cctataccgc
    15781 tacatcgagc cacaactgcc ggatgtggcg acattaaaag atgttcgcct gcaaattccg
    15841 atgcagattt acagcgccga tggcgagctg attgctcaat acggtgagaa acgtcgtatt
    15901 ccggttacgc tggatcaaat cccgccggag atggtgaaag cctttatcgc gacggaagac
    15961 agccgcttct acgagcatca cggcgttgac ccggtgggga tcttccgtgc ggcaagtgtg
    16021 gcgctgttct ccggccacgc gtcacaaggg gcgagtacca ttacccagca gctggcgaga
    16081 aacttcttcc tcagtccaga acgcacgctg atgcgtaaga ttaaggaagt cttcctcgcg
    16141 attcgcattg aacagttgct gacgaaagac gagatcctcg agctttatct gaacaagatt
    16201 taccttggtt accgcgccta tggtgtcggt gctgcggcac aagtctattt cggaaaaacg
    16261 gtcgaccaac tgacgctgaa cgaaatggcg gtgatagctg gactgccgaa agcgccttcc
    16321 accttcaacc cgctctactc aatggatcgt gccgtcgcgc ggcgtaacgt cgtactgtcg
    16381 cggatgctgg atgaaggata tatcacccag caacagttcg atcagacacg cactgaggcg
    16441 attaacgcta attatcacgc gccggagatt gctttctctg cgccgtacct gagcgaaatg
    16501 gtgcgccagg agatgtataa ccgttatggc gaaagtgcct atgaagacgg ttatcgcatt
    16561 tacaccacca tcacccgcaa agtgcagcag gccgcacagc aggcggtacg taataacgtg
    16621 ctggactacg acatgcgcca cggctatcgc ggcccggcaa atgtgctgtg gaaagtgggc
    16681 gagtcggcgt gggataacaa caagattacc gatacgctga aggcgctgcc gacctatggt
    16741 ccgctgctgc ctgccgcagt caccagcgcc aatcctcagg aagcgacggc aatgctggcg
    16801 gacgggtcga ccgtcgcatt gagtatggaa ggcgttcgct gggcgcgtcc ttaccgttcg
    16861 gatactcagc aaggaccgac gccgcgtaaa gtgaccgatg ttctgcaaac gggtcagcaa
    16921 atctgggttc gtcaggttgg cgatgcatgg tggctggcac aagtgccgga agtgaactcg
    16981 gcacttgtgt cgatcaatcc gcaaaacggt gccgttatgg cgttggtcgg tggctttgat
    17041 ttcaatcaga gcaagtttaa ccgcgccacc caggcactgc gtcaggtagg ttccaacatc
    17101 aaaccgttcc tctacaccgc ggcgatggat aaaggtctga cgctggcaag tatgttgaac
    17161 gatgtgccaa tttctcgctg ggatgcaggt gccggttctg actggcagcc gaagaactca
    17221 ccaccgcagt atgccggtcc aattcgctta cgtcaggggc tgggtcagtc gaaaaacgtg
    17281 gtgatggtac gcgcaatgcg ggcgatgggc gtcgactacg ctgcagaata tctgcaacgc
    17341 ttcggcttcc cggcgcaaaa cattgtccac accgaatcgc tggcgctggg ttcagcgtcc
    17401 ttcaccccaa tgcaggtggc gcgtggctac gcggtcatgg cgaacggcgg cttcctggtg
    17461 gacccgtggt ttatcagcaa aattgaaaac gatcagggcg gcgtgatttt cgaagcgaaa
    17521 ccgaaagtag cttgcccgga atgtgatatt ccggtgattt acggtgatac gcagaaatcg
    17581 aacgtgctgg aaaataacga tgttgaagat gtcgctatct cccgcgagca gcagaatgtt
    17641 tctgtaccaa tgccgcagct ggagcaggca aatcaggcgt tagttgcgaa gactggcgcg
    17701 caggagtacg caccgcacgt catcaacact ccgctggcat tcctgattaa gagtgctttg
    17761 aacaccaata tctttggtga gccaggctgg cagggtaccg gctggcgtgc aggtcgtgat
    17821 ttgcagcgtc gcgatatcgg cgggaaaacc ggtaccacta acagttcgaa agatgcgtgg
    17881 ttctcgggtt acggtccggg cgttgtgacc tcggtctgga ttggctttga tgatcaccgt
    17941 cgtaatctcg gtcatacaac ggcttccgga gcgattaaag atcagatctc aggttacgaa
    18001 ggcggtgcca agagtgccca gcctgcatgg gacgcttata tgaaagccgt tcttgaaggt
    18061 gtgccggagc agccgctgac gccgccgccg ggtattgtga cggtgaatat cgatcgcagc
    18121 accgggcagt tagctaatgg tggcaacagc cgcgaagagt atttcatcga aggtacgcag
    18181 ccgacacaac aggcggtgca cgaggtggga acgaccatta tcgataatgg cgaggcacag
    18241 gaattgttct gattaaaaag gcgcttcggc gccttttcag tttgctgaca aagtgcactt
    18301 gtttatgccg gatacgggtg aacgcgttat ccggctaaca aaatcgtgaa aactcaatat
    18361 attgcaggaa ccccataagc ctgataagcg ttgtgcatca ggcaaacttc acgcatttac
    18421 actcgcccct gccctttcaa ccattcgcgc acgaggaaca gcgcgctgac attacgcgct
    18481 tcgttgaagt cagggtcttc cagcaaatcc atcatatgcg ccagcggcca gcgcacctgt
    18541 ggtagcggct ctggctcatc gccttccagc gattccgggt agagatcttg cgctaccact
    18601 atattcattt tgctggaaaa gtaagacggt gccatgctga gcttcttcaa aaaagtcaga
    18661 tcgttcgcac caaatccaac ctcttctttt agctcgcggt tagcggcttc gtagacgctt
    18721 tcacccggat caattaatcc tttcgaaaaa cctaattcgt aggattcagt tcccactgcg
    18781 tattcgcgga tcaggatcag gtgatcgtcc acaatcggca caatcatcac tgcttcccgg
    18841 ttggttggac gcattcgttc ataaacacgc cgcacgccat tgctgaactc cagatccacg
    18901 ctctcgacgg taaacagtcg ggaacgggct acagtttcaa cattcagaat ggtgggtttt
    18961 tgtaatgatt tgctcatcgt gggatctatg ctgtgaaatc agcggttatt gtgcgatatc
    19021 ggacacgctt tcggcaatgt gaattgcatg ttatttacat ttatgtaact taataaataa
    19081 tcgtcctcaa atcaaattaa aagtcaatag gttgaaataa ctccaggaat ttgctgatat
    19141 tccgccttca gagggtttgc tatgatcagc ggttactgtg atgtgcttaa tgatgctcaa
    19201 gttaaactcc acgcttgccg atagccaacc gcagaatcat gtattgtgtc cggtgcgact
    19261 gaccacgcct gacagactaa gtaagatggg gaaagcatga gcaccattgt gattttttta
    19321 gctgctttgc tggcctgctc actacttgcg ggatggctga taaaagtgcg atccagacgg
    19381 cgtcagctgc cctggaccaa cgccttcgcg gatgcgcaaa cgcgtaaact cacacctgaa
    19441 gaacgtagcg ccgttgaaaa ttatcttgag agcctgacgc aggtattaca ggtgcctggc
    19501 ccaacgggag ccagcgcggc accgatctct ctggcgctga atgccgaaag caacaacgtc
    19561 atgatgctga cacacgctat cacacgttac ggcatctcta ccgacgatcc gaataaatgg
    19621 cgttactacc tcgattcggt agaagtccac ctgccccctt tctgggaaca gtacatcaac
    19681 gatgagaata ccgttgaact gattcatacc gattcgctgc cgttggttat ttcactcaac
    19741 ggtcatacgc tgcaggagta catgcaggaa acgcgcggct ataccttgca acctgttccg
    19801 tccacgcagg cgtcgattcg cggggaagag agtgagcaaa tcgagctact caatattcgc
    19861 aaagaaacgc atgaagaata tgcgctgagt cgtccgcgcg ggctgcgtga agcgttgctg
    19921 atcgtcgcct cctttctgat gttctttttc tgcctgatta ccccggatgt atttgttccg
    19981 tggctggcag gcggcgcgtt actgctgctg ggcgcaggtc tgtgggggct attcgccccc
    20041 ccggcaaaat cctccctgcg ggaaattcat tgtctgcgcg gtacaccccg tcgttgggga
    20101 ttgtttggcg aaaacgatca ggaacagatc aacaatattt cactcggtat tatcgacctg
    20161 gtctatcccg cacactggca gccatacatt gctcaggatc tcggtcaaca aaccgatatt
    20221 gatatctatc tcgaccgcca cgtagtgcgt caggggcgat atctttcgct gcatgatgaa
    20281 gtaaaaaact ttccgttaca gcactggctg cgcagtacga ttatcgctgc gggctcgctg
    20341 ctggtgctgt ttatgctgtt attctggatc ccgctggata tgccgctgaa attcactctc
    20401 tcatggatga aaggcgcgca gaccattgaa gccaccagcg taaaacaact ggctgacgct
    20461 ggcgtgcggg taggcgatac attgcgtatt agcggtacgg gaatgtgtaa tattcgcact
    20521 tccggaacct ggagcgcgaa aaccaattca ccttttttac cgtttgactg ctcgcagatc
    20581 atctggaatg acgcccgctc attaccatta ccagaatctg aactggtcaa caaagcgacg
    20641 gcattgactg aagcagttaa tcgccagctg caccctaaac cggaagatga atctcgcgtc
    20701 agcgcctcat tacgttcagc aattcaaaaa tccggcatgg tattgcttga tgattttggc
    20761 gacattgtac tgaagacagc ggatctgtgt tctgccaaag atgactgtgt gcgactgaaa
    20821 aatgcgctgg tcaatctcgg caacagtaaa gactgggacg cgctggtaaa acgcgccaac
    20881 gccgggaaac tcgatggcgt gaatgtgtta ttacgcccgg taagtgcgga atcgctggat
    20941 aacctggtgg caacctccac cgcgccgttt atcacgcatg aaacggcacg agcggcacaa
    21001 tcactaaaca gtccggcccc cggcggattc ctgattgtca gcgatgaagg cagcgatttt
    21061 gttgatcaac cctggccttc ggcatcactt tacgactacc cgccgcaaga acagtggaac
    21121 gctttccaga aactggcaca aatgctgatg catacgccgt ttaacgccga gggtatcgtc
    21181 acaaaaatct tcactgacgc caatggtacg cagcatatag gccttcatcc gatcccggat
    21241 cgttccggcc tgtggcgcta tctcagcacc acactgctgc tactaacgat gctgggtagc
    21301 gccatttata atggcgtaca ggcctggcgg cgttaccagc gtcatcgcac tcgcatgatg
    21361 aagattcagg cctattatga aagctgcctg aacccgcaac tgatcacccc ttcagaaagc
    21421 cttatcgaat aacacgtttg cgcggcaggt tatgctaccc tgtcgcgcaa attgcttcac
    21481 tctggagatt tccctcatgc atatcaacat tgcctggcag gacgtagata ccgttctgct
    21541 ggatatggac ggcacgttgc tcgacctcgc cttcgataac tatttctggc aaaagctggt
    21601 gcctgaaaca tggggcgcga aaaacggggt tacgccacag gaagcgatgg aatatatgcg
    21661 ccagcaatat cacgacgtac agcatacgct aaactggtac tgtcttgatt actggagtga
    21721 gcaactgggt ctggatatct gtgcgatgac caccgagatg ggaccgcgtg ccgtactgcg
    21781 tgaagatacc attccgtttc ttgaggcact gaaagccagc ggtaagcagc gaattttgct
    21841 caccaatgcg catccgcaca acctggcggt aaaacttgag cataccggtc tggacgcaca
    21901 ccttgattta ttactttcca cccacacatt tggttatccg aaagaggatc agcggttatg
    21961 gcatgcggtg gccgaagcta cgggtctgaa agctgaaaga acgctgttta ttgatgacag
    22021 cgaagcgatt ctcgatgctg ccgcgcaatt tggtattcgt tactgcctcg gcgtgactaa
    22081 tcctgactca gggattgccg agaaacagta tcaacgccat ccgtcactga atgactaccg
    22141 ccgcctgatc ccctcgctaa tgtgaaggag acgccatgaa agaaaaacct gctgttgagg
    22201 ttcgactgga taaatggcta tgggctgccc gtttttataa aacccgcgcg ctggcccgtg
    22261 aaatgattga aggcggtaag gtgcattaca acgggcagcg cagcaagccg agcaaaatcg
    22321 tcgagctgaa tgccacgctc actctgcgcc agggaaatga cgaacgcacg gtgattgtaa
    22381 aggcgattac tgaacagcgt cgccccgcca gcgaggcagc cttgctgtat gaagagactg
    22441 cggaaagtgt agagaaacgc gaaaaaatgg cgctggcacg taaacttaat gccttaacca
    22501 tgccgcatcc ggaccgacgc ccggacaaaa aagagcgccg cgacctgtta cgatttaaac
    22561 acggcgacag tgaataactg tcacctgcaa gagagatgat tatgccgcaa catgaccaat
    22621 tacatcgcta tctgtttgaa aactttgccg ttcgcggcga actggtaacc gtttcggaaa
    22681 ccctgcaaca gatccttgag aatcacgatt atccgcagcc cgttaaaaac gtgctggcag
    22741 aactgctggt tgcgaccagc ctgttaaccg ctacgctgaa gtttgatggt gatatcaccg
    22801 tacagctgca aggcgacggt ccgatgaatc tggcggttat taacggtaac aacaaccagc
    22861 agatgcgcgg tgtggcgcgc gtgcagggcg aaattccaga aaatgccgac ctgaaaacgc
    22921 tggtcggcaa tggttacgtg gtgatcacca ttaccccgag cgaaggtgaa cgctatcagg
    22981 gcgtggtggg tctggaaggt gataccctgg cggcctgcct ggaagattac tttatgcgtt
    23041 ctgaacagct gccgacgcgt ctgtttattc gcaccggcga cgtagacggc aaaccggctg
    23101 caggcggtat gttgttgcag gtaatgcctg cgcaaaatgc ccagcaggac gactttgacc
    23161 acctggcgac gctaaccgaa accatcaaaa ccgaagaact gctgacctta ccggcaaacg
    23221 aagtgttgtg gcgtttgtat cacgaagaag aggtgacggt ttacgatccg caggatgtgg
    23281 agttcaaatg cacctgctcg cgtgaacgtt gcgccgatgc gctgaaaacg ctgcctgatg
    23341 aagaagtcga tagcatcctg gcggaagatg gcgaaattga catgcattgt gattactgcg
    23401 gtaaccacta tctgttcaat gcgatggata ttgctgaaat ccgcaacaac gcgtctccgg
    23461 cagatccgca agttcattaa tgagtatgtc cggcagagag ggttctgccg gattttacga
    23521 ctaatacacc tgatgcgctt tgtcactcat tcagattcac atccagcctt tcaccatcca
    23581 ctacaagatc gcgccacgct ttgggcttcg ttcccagttt tccatccttc gcagctgtga
    23641 gtaatttttc tttcgttata tcacgcggaa gtaaactcat cgtaagctct tgccattctt
    23701 tcttgtcagg atcaaagcca tagacaatgt atctttcatc atcaaaggca aagaggatcc
    23761 gctctgccct gccatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa
    23821 tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt
    23881 taccagaacc aggggcaatt aacacatttt cggctaatac tttttccgat atctgttgct
    23941 gaagatgttg ctctccatct aacgccatca acagatcccg cgcacgtttc gggtctttca
    24001 taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt
    24061 cgagcatgta gatagttacc tggtctggcg tgtttttgcc actctgataa cgcgccatat
    24121 gactgttcac gctaatgcgc atactgtcca gcaccggaga attaagaagc accagtatta
    24181 ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc
    24241 ctttacgcca tacgatactg acaaaatacc ccagcgacca taccagtaac accagcaccg
    24301 ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacg
    24361 cccaggcggc gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac
    24421 gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga
    24481 tcaattgcaa aaaggccagc gttaacagca acccagcggc ggagatgtgg cgagaaatcg
    24541 cactcaggcc tgtaaacggc agggtgatga taaacatcag ggttagcaat gatactaacg
    24601 gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat agagtcgatt aaacgtgact
    24661 gtgttcgcgc gaggatcacc gccagcgcgg taaccagacc taatgtaaga tacataaacc
    24721 agtcggttgc aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta aacaactcgc
    24781 cccacagtaa aagcaccagc cacgtcaggc cattggcgag aaaaatcacc agtaatataa
    24841 gtatgttatg ccatactgac tggtaaaagt agctatagcg gctactgtca ttgcggatac
    24901 gaaggctttg ttgtatccac ggtagcaaca acattgccat cagcagcaga tagcagccaa
    24961 aatcccaaag cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcaacc
    25021 agccgctcat ccccagcgtg gcaataaaca ccagcgccag ccatccccaa aggcgcttct
    25081 gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcaacggtc gcaggcacgc
    25141 cataaacaat ccaactgtga ttttttccgg caagccaggc gatcagcaga tagcaaacca
    25201 gcccctgaag taatccggta gcaatcatcc cccagcgtgt tgcgggtgaa agttcaacgt
    25261 tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta
    25321 ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccgg
    25381 ttgaatttgc atcaatttca ttcaggaatg cgattccact cacaatattc ccgccatata
    25441 aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat
    25501 agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata
    25561 cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa
    25621 tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc cgcaagaact
    25681 cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct acgacctgct
    25741 gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt taactaatct
    25801 gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag ataagtatat
    25861 cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa
    25921 cgataacaaa cctctctctc cggaaacctg gcaacatctg aaaggcctgg tgaccaaaca
    25981 gctttccggc aaacgtctgt tcgttgtcga cgccttctgt ggtgcgaacc cggatactcg
    26041 tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt
    26101 tattcgcccg agcgatgaag agttggcagg tttcaaacca gactttatcg ttatgaacgg
    26161 cgcgaagtgc actaacccgc agtggaaaga acagggtctg aattccgaaa acttcgtggc
    26221 ttttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa
    26281 gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg cttctatgca
    26341 ctgctccgcc aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac
    26401 tggtaaaacc accctttcca ccgacccgaa acgtcgcctg attggcgatg acgaacacgg
    26461 ctgggacgat gacggcgtgt ttaacttcga aggcggctgc tacgcaaaaa ctatcaagct
    26521 gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgcgatgcgc tgctggaaaa
    26581 cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa ccgagaacac
    26641 ccgcgtttct tatccgatct atcacatcga taacatcgtt aagccggttt ccaaagcggg
    26701 ccacgcaacg aaagttatct tcctgaccgc agacgccttt ggtgtgctgc cgccggtttc
    26761 tcgtctgact gccgatcaaa cccagtacca cttcctctct ggcttcaccg ccaaactggc
    26821 cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct tcggcgcggc
    26881 attcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta tgcaggcggc
    26941 gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac gtatctcgat
    27001 taaagatacc cgcgccatta tcgacgctat cctcaacggt tcgctggata atgcagaaac
    27061 cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacacgaa
    27121 gattctcgat ccgcgtaaca cctacgcttc cccggaacag tggcaggaaa aagccgaaac
    27181 cctggcgaaa ctgtttatcg acaactttga taaatacacc gacacccctg cgggtgccgc
    27241 gctggtagcg gctggtccga aactgtaatg acttgaagct ggagaatatc tatccagtat
    27301 cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct tcttttgtcg
    27361 tgccctgcgc ccgcgttacc ggcacaggca gccaggcgcg aatggaaagc ccgccccgct
    27421 cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc acaatcgcca
    27481 gccctaatcc cgtgccgcta atggtgcgtg cactgtcgcc acgaacaaac ggctggaaaa
    27541 ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg aaccaggcgc
    27601 gattcggctc cgtcccgctg ctgactttga tccagccatt gccgtaacgg gcggcgttga
    27661 ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcact tcaatgctgc
    27721 cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca ataacctcac
    27781 cgagtactgc gttgagatcc gccatttcca tcggcatctc ctgcccggtg cgcaggtagt
    27841 cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc gattctgcca
    27901 ggtagccatc ctgctcgctc atcatctcgg tcgccaggcg aatacgcgtc agcggtgtac
    27961 gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt tgcttaacac
    28021 cagccgccat atggttaaag gcgcgggtaa cggaacgcac ctcggacgcg ccatactcac
    28081 gcagcggcgg cggaataatc cctttaccga cctgcaaagc agcatgttcg agatcgacca
    28141 acggtcggtt ctggatacga ataaacagcc acgccccgcc tatcgccaat agcataatcg
    28201 ccaacgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc agcggcacgc
    28261 gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa cttttgttga
    28321 cctcaacgcg cacttccgtc gggccgccca gttgctgtgc catctgatgg cttaagaatt
    28381 catagtgttg tgcccaacgc agacctgcct cttcggcagc ttcgttggaa tagagagaga
    28441 tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac tgcgtgccgt
    28501 cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg actttattaa
    28561 actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc gtcaccaggc
    28621 tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt cgtggcgaga
    28681 agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca ggccccaaac
    28741 ggtctgaatg taacgcggat gcgctggatc ttcttccacc atgcggcgca gacgcgaaat
    28801 ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa ggttcatcag
    28861 cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta ccgcaaactc
    28921 accgctggtg agcggcatcg gctcgtcttc gcggaacatt tcgcgcgtac cgaggttaag
    28981 tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg gtagctcgtt
    29041 cgcctgacga cgcagcaccg cacggatacg ggccagcagt tcacgcgggt taaacggttt
    29101 tggaatgtaa tcgtcagcgc caatctccag gcctacgata cggtccactt cttccccttt
    29161 cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc ggcaaatcga
    29221 caagccatct tcaccaggta acatcaaatc cagtaccata agatggaaag attcacgagt
    29281 cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc cttgttcggt
    29341 gagataacgc tccagcagcg cgcgcaggcg catgtcgtca tcgaccacca gaatcttgta
    29401 gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta
    29461 aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa attgttacaa
    29521 agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat tgattctgtt
    29581 gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga
    29641 tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag cttaattatc
    29701 tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca agtctgggcg
    29761 accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa atcgaccgtc
    29821 gcgtgcgcta tctcactaaa tgcatggaaa atctcaaaat cgtcgattac tcccctcagc
    29881 aggaaggcaa agtctttttt ggcgcgtggg tggagattga aaacgacgat ggcgtgactc
    29941 accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac atctctatcg
    30001 attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggct gtggtgaata
    30061 ctcctgccgg ggaagcgagc tggtatgtta atgctatcga gtacgtgaaa ccgtaaggaa
    30121 gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat aactatcccc
    30181 tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc tgccgcatta
    30241 ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc ctgcttgacg
    30301 aagggaatac cgtgcctttt attgcacgtt atcgtaagga aatcaccggc ggtctggatg
    30361 acacgcagct gcgtaatctg gaaacgcgtc tgggatacct gcgcgagctg gaagagagac
    30421 gtcaggcgat cctcaagtcc atttccgagc aaggcaaact caccgatgat ctggcgaatg
    30481 ccatcaacgc caccctaagc aaaaccgaac tcgaagacct ctacctgccc tacaaaccta
    30541 aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg gctgacctgc
    30601 tgtggagcga tccgtcacac acgccagaag tcgccgctgc gcaatatatt gatgccgata
    30661 aaggcgtggc agataccaaa gccgcgctgg acggcgcgcg ctatatcctg atggaacggt
    30721 ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag aacgcgcatt
    30781 tggtttctac ggtggtgagc ggtaaagaag aggaaggggc gaaattccgc gactatttcg
    30841 atcatcacga accgttgtcc acggtgcctt ctcaccgcgc gctggcgatg ttccgtgggc
    30901 gtaacgaagg cgtacttcag ctttcgctga atgccgatcc acagttcgat gagccgccga
    30961 aagagagcta ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg aacaatgctc
    31021 cggcggatag ctggcgcaaa ggcgtggtga gctggacctg gcgcatcaag gtgctgatgc
    31081 atctggaaac cgaactgatg ggcaccgtgc gcgaacgcgc ggaagatgaa gcaatcaacg
    31141 tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg cgtgcaacga
    31201 tgggcctcga tccgggtctg cgtactgggg taaaagtggc ggtggtcgat gccactggca
    31261 aactggtagc gaccgatacc atttacccgc acaccggaca agccgcaaaa gcagcgatga
    31321 ctgttgctgc gctgtgtgaa aagcataacg ttgaactggt ggcgatcggc aacggtacag
    31381 cttcccgcga aaccgaacgt ttctatctcg acgtgcagaa gcagttcccg aaagtgaccg
    31441 cacagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcggcttcc gagctggcag
    31501 cgcaggagtt cccggatctc gacgtttcgc tgcgtggcgc ggtttctatc gcccgccgtt
    31561 tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc gtaggtcagt
    31621 atcagcatga cgtcagccag acgcaactgg cccgcaaact ggatgcagta gtagaagact
    31681 gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt tccgctatta actcgcgtgg
    31741 cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag aacggccagt
    31801 tccagaaccg ccagcaactg ctgaaagtca gccgtctggg gccgaaagcc ttcgagcagt
    31861 gcgcgggctt cctgcgcatt aaccacggtg ataacccgct ggacgcctct accgttcacc
    31921 cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca ctgaaagatc
    31981 tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact gatgaaaaat
    32041 tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt cgcgatccgc
    32101 gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac gacctgcaac
    32161 cgggtatgat cctcgaaggc gctgtcacca acgtcaccaa ctttggcgcg tttgtcgata
    32221 tcggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag tttgtggaag
    32281 atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg gaagtggatc
    32341 ttcagcgtaa acgtatcgcc ctgactatgc gtctggacga gcagcctggc gaaaccaact
    32401 ctcgtcgcgg cggcggtaac gatcgcccgc aaaacaaccg cccggcagcc aaaccacgcg
    32461 gtcgtgaagc gcagcctgcc ggtaacagcg cgatgatgga cgcgctggcg gcagcgatgg
    32521 gtaaaaaacg ttaaacgccc ggactggcct acggttcgaa tttgcacgaa atcgtagggc
    32581 agataaggcg ttcacgccgc atctggcaac gaacgccttg tctgacataa aagtgccgga
    32641 gaatatctcc ggcatattta ttccacagcc aaactcacaa tatattccgt taatatttat
    32701 cacttcatta acaactgaaa ccttaattaa acattagcca gtcctggtaa ttcgctattc
    32761 gaattatatt ttcgctgcga tataaccttg agccacatca acattgagtc agattattat
    32821 tcaaaccaac attcgcacac attttaagta ttgctgatag aaaccattct cattatcatt
    32881 gtactgttga ttatttaatc tctccttcgt tggcaaatca tctggtctca tgtcgctgtc
    32941 aaacgcccca tgaggtagtt atccagttaa tgagaaacaa gtaggcacct atgcaataca
    33001 ctccagatac tgcgtggaaa atcactggct tttcccgtga aatcagcccg gcatatcgcc
    33061 aaaaactgct ttctcttggc atgttacctg gctcctcttt taatgtggtg cgcgtcgctc
    33121 cactcggcga ccccattcat atcgaaaccc gtcgtgtgag cctggtatta cgcaaaaaag
    33181 atctggcctt attagaagtg gaagcggttt cctgttaata cggtgataac aacaatgaaa
    33241 aaattaacca taggcttaat tggtaatcca aattctggca agacaacctt atttaaccag
    33301 ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg ttaccgtcga acgtaaagaa
    33361 gggcaattct ccaccaccga tcatcaggtc acgctggtgg acctgcccgg cacctattct
    33421 ctgaccacca tctcatcgca gacctcgctc gatgagcaaa tcgcctgtca ctacattttg
    33481 agtggcgacg ccgacctgct gattaacgtg gtggatgcgt ctaaccttga gcgtaacctg
    33541 tacctgacgc tacaactgct ggaactcggc attccctgca tcgtggcact gaacatgctc
    33601 gacattgccg agaagcaaaa tattcgtatt gaaattgatg ctctgtcggc gcgtcttggc
    33661 tgcccagtga tcccgctggt ttcaacccgt ggtcgcggta ttgaagcgct caagctggcg
    33721 attgatcgct ataaagctaa cgagaatgtg gaattggtgc attacgcaca gccgctgctc
    33781 aacgaagcag attcactggc aaaagtgatg ccttccgaca tcccgctgaa acaacgtcgc
    33841 tggctgggcc tgcaaatgct ggaaggcgat atctacagcc gcgcctacgc cggtgaagcg
    33901 tcgcagcatc tggatgccgc cctcgcccgt ctgcgtaatg agatggacga tccggcgctg
    33961 cacattgccg atgcgcgtta ccagtgcatt gctgccatct gtgatgtggt aagcaacacc
    34021 ctgacggcag aacccagccg tttcaccact gcggtagata aaatcgtgct caaccgtttc
    34081 ctcggtctgc cgattttcct ctttgtgatg tacctgatgt tcctgctggc tatcaacatc
    34141 ggcggggcgt tacagccgct gtttgacgtc ggctccgtgg cgctatttgt gcatggtatt
    34201 caatggattg gctacacgct ccacttcccg gactggctga ctatcttcct cgcccagggc
    34261 ctgggtggcg gtattaacac cgtgctgcca ctggtgccgc agatcggcat gatgtacctg
    34321 ttcctctcct tccttgagga ctccggctat atggcgcgtg cggcgtttgt gatggaccgc
    34381 ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc cgctgatcgt cggtttcggt
    34441 tgtaacgtac cgtcggtaat gggcgcacgt acgcttgatg caccgcgtga acgtctgatg
    34501 accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc tggctatctt cgcagtattc
    34561 gcggctgcct tcttcgggca gaacggtgcg ctggcggtct tctcgctgta tatgctcggt
    34621 atcgtgatgg cggtgctgac tggcctgatg ctcaagtaca ccatcatgcg cggtgaagcg
    34681 acgccgtttg tcatggagct gccggtctac catgtaccac acgttaaaag cctgattatc
    34741 cagacctggc agcgtctgaa aggcttcgtt ctgcgtgctg gtaaagtgat tatcatcgtc
    34801 agcattttcc tgagcgcttt caacagcttc tcgttgagcg ggaaaatcgt cgataacatc
    34861 aacgactcgg cgctggcttc cgtcagccgg gtgatcaccc cggtcttcaa gccgattggc
    34921 gtgcatgaag ataactggca ggcaacagtt ggcctgttta cgggtgcaat ggcgaaagaa
    34981 gtggtggtgg gtacgctcaa caccctctac accgcagaaa atattcagga cgaagagttc
    35041 aatccggcgg aatttaacct cggtgaagag ttgttcagcg cggtagatga aacctggcag
    35101 agcctgaaag acaccttcag ccttagcgta ctgatgaacc ccattgaagc tagcaaaggc
    35161 gacggcgaaa tgggtaccgg ggcgatgggc gtgatggatc agaaattcgg tagcgcagct
    35221 gccgcataca gctacctgat tttcgtcctg ctgtatgtac catgtatctc ggtgatggga
    35281 gccatcgccc gtgaatcaag ccgtggctgg atgggcttct ccatcctgtg ggggctgaat
    35341 atcgcttact cactggcaac attgttctat caagtcgcca gctacagtca gcacccaact
    35401 tacagcctgg tgtgcattct ggcggttatc ctgtttaaca tcgtggttat cggtctgctg
    35461 cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa cccgcaagtc ggtaagtagt
    35521 tgctgcgcag ccagcaccac cggtgattgc cattaatggc ttcacttatt caggtgcgtg
    35581 atttgctggc gttacggggc cgtatggaag cggcccagat aagccagaca ttgaacactc
    35641 cacagccaat gattaacgcc atgctgcaac aactggaaag tatgggcaaa gccgtgcgga
    35701 ttcaggaaga acctgacggc tgcctctccg gcagttgtaa aagctgcccg gaaggaaaag
    35761 cctgtctgcg cgagtggtgg gcgctgcgtt aaccatactc catcgcctgt tttgaaaagc
    35821 ccggtatgcg tttgcatccg ggcttttttg cgtgcggctt tccataaaaa tgcaattctt
    35881 gcagcgcgac gtaagttcct ctgaaagcgt ctcgcaggga tgaaaactgg ctaatgcaca
    35941 ggtgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca ggtgaaggaa cgccatgagc
    36001 aaaaagcaga gttccacccc acacgatgcg ctgttcaaac tctttttacg ccaaccggag
    36061 acggcacgcg attttcttgc gtttcattta ccggcaccca ttcacgcgct ctgtgatatg
    36121 aaaaccctca agctggagtc gagcagcttt attgatgacg atctgcgtga aagctattcc
    36181 gatgtgctgt ggtcggtgaa aacagaacaa ggaccaggat acatctattg tctgattgaa
    36241 catcaaagca cctcaaacaa actgatcgca tttcgcatga tgcgttacgc tattgccgca
    36301 atgcaaaatc accttgatgc cggatacaaa acgttgccga tggtggtgcc attgttgttt
    36361 taccacggta ttgaaagccc ctatccctat tcgctgtgtt ggctggattg tttcgccgat
    36421 cccaaactgg caaggcagct ttatgcctcc gcatttccgc tgattgatat caccgtcatg
    36481 cctgatgatg aaatcatgca gcaccgacgc atggcgctgc tggagttaat tcaaaaacat
    36541 attcgtcaac gcgacctgat ggggctggta gagcaaatgg cctgcttatt aagtagtgga
    36601 tacgctaatg acagacaaat caaagggctg tttaattaca tactgcaaac cggcgatgcg
    36661 gtacgtttta acgattttat cgacggcgtt gccgaacgat caccgaaaca caaggagagt
    36721 ttaatgacta ttgcggaaag attgcggcag gagggggaac aatccaaagc cctgcatata
    36781 gccaaaataa tgcttgaatc cggagtccct cttgcagaca tcatgcgctt taccgggctg
    36841 tcagaagaag agttggctgc ggcgagtcag taaagttctg tctcgccatt caaaagtgcc
    36901 tacaccctct gcttcaacgc caccagcagg tgacgaaact cgaccggatg cgaaataaac
    36961 ggcgcatgag ccgctttggc gaagatgtat gactcgctgt gtggccagag tttatccagc
    37021 atcggcacca ctttgcgcgg caccagacca tcgagatagc catacagtcg caaaaacggc
    37081 atgggtacgt tttgcagcgg caagcggaga tcaaccgttt tgaggatttc cagcccacca
    37141 ttgagcacgt caacctccgg catcggtaac gccagaacgg ttttcttcaa cgcccgcgca
    37201 tcctggcggg ccgtttcagt acccatggtt tgtaacgcca ggaaccgttc caccgtacgc
    37261 tgaaaatcat cactgagttg ctgctgaaat cccgccaaca cctccggttt gatccccggc
    37321 cactcttcac gagcgctgaa acacggcgac gacgccacgg tgaccagcgc ctgaacacgc
    37381 tcgggatggg ttaacgcaat ctggcttgcc accagcccgc ccagactcca gcctaaccaa
    37441 atggctttat caggtgcctg tcgcagcacg gcttcggcca tctccgcaag tgacagcgca
    37501 ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa gatgcagcgt aaaatgcgag
    37561 ctaagttcct cgtcaatgca acgccacact tcggcattca gtccccatcc gtgcagcagc
    37621 acaagatgaa cattcccctg acctttggtc tgccaccaga tgttattcat ccgctattgt
    37681 tctcttttga cttacaagga tgaacatatg ctaacagtac cgggattatg ctggctatgc
    37741 cgaatgccgc tggctttagg tcattggggg atttgttcgg tctgctcacg cgccgcccgt
    37801 acagataaaa cgttatgccc acaatgtgga ttacccgcca cacactccca tcttccctgc
    37861 ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg tcacggttgc cgactatgcg
    37921 ccgccgttaa gtctgctcat ccaccagctt aagttttccc gccgcagcga aatcgccagc
    37981 gccctgtcac gtctgttact attggaagtc ttacacgctc gtcgcaccac cgggttgcaa
    38041 ttgccggatc gtatcatcag cgttccgtta tggcagcggc gtcactggcg tcggggattt
    38101 aatcagagcg atttgctgtg tcagccgtta tcacgctggt tgcactgcca atgggatagc
    38161 gaagccatca cacgtacacg ggccactgcg acccagcatt ttcttagcgc ccggctacgc
    38221 aggcgcaacc tgaaaaatgc ctttcgcctt gaattgcctg tgcaaggtcg ccatatggtg
    38281 attgtggatg atgtcgttac caccggaagt accgtcgcag agattgcgca gttgctttta
    38341 cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc gaaccttgta gagcctcgat
    38401 gatgggcgta ttataaccaa ctaaaatagt caactattag gccattacta tgatccgtat
    38461 ttccgatgct gcacaagcgc actttgccaa actgctggca aatcaggaag aagggacaca
    38521 aatccgcgta tttgtgatta accctggcac gcctaacgct gaatgtggtg tttcttattg
    38581 tccgccggat gccgtggaag ccaccgacac cgccctgaaa tttgacctgc tgaccgcgta
    38641 tgttgatgag ttaagcgcac catacctgga agatgcagag atcgattttg ttaccgacca
    38701 gttgggttct cagctaacac tgaaagcgcc taacgccaaa atgcgtaaag tggcagacga
    38761 tgcaccactg atggagcgcg tggagtatat gctgcagtcg cagatcaacc cacagcttgc
    38821 tggtcacggt ggccgcgttt cactgatgga aatcaccgaa gacggttacg ccattctgca
    38881 atttggcggc ggctgtaacg gttgttccat ggtcgatgtg acgctgaaag aagggatcga
    38941 gaagcagcta ctaaacgaat tcccggagct gaaaggtgtg cgcgatctca ccgaacacca
    39001 gcgcggcgaa cactcctact actaagttat cccctctttt gtagattgcc cgatgcgacg
    39061 ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga cgttggcgca gcattgcttt
    39121 ggcaaatccc tcccttcgtt tccccgcgat aatatgacca acctctcata atttaaattt
    39181 accccgctct ggtgattctc aaacgccaga tgttacccgt atcattcaca tgggtaccaa
    39241 acatactcct gacatctgac tacaataatt agttttagtg ggtatcagtc gtggtgccgc
    39301 aatatctctg ttcccgattg ggataattag agtttgtcgt cagaaaattg acgttaccca
    39361 taacaaatga aaggccaggt aaatcatgcc attagtcatt gttgctatcg gtgtaatctt
    39421 gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc atcgctctcg tcctcgtggc
    39481 gcttgctgtt ggattaatgc aaggaatgcc gctggataaa gttattggct ccatcaaagc
    39541 cggtgtcggc gggacgctcg gtagccttgc cctgatcatg ggttttggcg caatgctggg
    39601 caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc accacgctga ttgccaaatt
    39661 tggtaaaaaa cacatccagt gggcggtggt actgaccggt tttaccgttg gttttgccct
    39721 gttctatgaa gtgggctttg tgctgatgct gccgctggtg tttaccatcg cggcttctgc
    39781 gaatattcca ctgctgtatg ttggtgtacc aatggcggct gcactgtctg tgacccacgg
    39841 cttcctgcca ccgcatccgg gtccgactgc gattgccacc attttcaatg ccgatatggg
    39901 taaaaccctg ctgtacggta ctattctggc aatcccgacc gtgattctcg ccggtccggt
    39961 ttacgctcgc gtgttgaaag gtatcgataa gccaattccg gaaggtctct acagcgcgaa
    40021 aaccttcagc gaagaagaga tgccgagctt tggcgtcagc gtctggacct ctctggtgcc
    40081 ggtagtactg atggcgatgc gtgcgattgc cgaaatgatc ctgccgaaag gtcacgcttt
    40141 cctgccggta gcggagttcc tcggtgaccc ggtaatggct acgctgattg ccgtgctgat
    40201 tgcgatgttc acctttggtc tgaaccgtgg tcgttcaatg gatcagatta acgacacgct
    40261 ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc atcggtggtg gcggtgcgtt
    40321 caagcaggtg ctggtagaca gcggcgtgga caaatacatt gcttccatga tgcacgaaac
    40381 caacatttct ccgctgctga tggcctggtc gattgctgcc gtactgcgta tcgcgctggg
    40441 ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg gcaccgctga ttgcaacgac
    40501 gggtgttagc ccggagctga tggttattgc ggttggttcc ggtagtgtga ttttctctca
    40561 tgtgaacgat ccgggcttct ggctgttcaa agagtacttt aacctgacta tcggcgagac
    40621 catcaaatcc tggtcgatgc tggaaaccat tatctcggtg tgcggtctgg taggctgtct
    40681 gctgctgaat atggtgattt gataacactg actgccggat gcatcgcatc cggcatcaac
    40741 cgcactctac ttcttcttcg ctgcagctct gcgccgtctg tccaaatcct tcagcaactt
    40801 gttcacgcca tcatcggcaa acatcgtctc aagcgttgcg gaaagcttgc gtcgccagtt
    40861 tttatactgg taactggtgc caggaatatt caccggttcg gccatatcca gccagtcttc
    40921 cggctgtagt cctaacagag cactgttact gtcggcaatg tagcgctgca aaccacggtt
    40981 cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg gcagacaacc
    41041 atatttatgc agtgcatcca gcagcccttg cttcgccagt tcgcgatcct gatacagacc
    41101 gcgcagtacc acttcatccg gatacagccc cagggttttg cccagcgtta gatccccgct
    41161 ctcccagtag ccgcgtagcg taggcaggtc atgtgtcgcc gcaaccgcca tcgactgctc
    41221 cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg ttttcgaaat agagcacttt
    41281 gtaggagtac acaccgcagc tgcgcagctt accgacaatc tctaccggta cggtaccgag
    41341 atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa tcgaaagcag
    41401 atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat acggtatcca
    41461 ccacaaacgc agcatcgaca tcacatggtc aattcgtaat gcgccgcagt tttgcatatt
    41521 ggcacgcaac agctcgataa acggttcata agcacgcgcg gtgatgatat gcgggtccat
    41581 tggcggcaat ccccagttct gccccaacgg gccgaggata tccggcggcg cgccaaccga
    41641 cgctttcagg caatacagtt cacggtcgca ccaggtttcc gccccacctt ccgctacgcc
    41701 aaccgccaga tcacgataca agccaatcgg catttcatag ccctggctta tctcccagca
    41761 ggcggcaaac tggctgtaag ccagccactg cagccagaga taaaaatcaa cgtcatcgcg
    41821 atgttcttcg cagaactgac gcacttctgg tgaatccacg ttctgataca tctctggcca
    41881 tgcaggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta gcgcatcaaa
    41941 ggctgcctgc cagaacaggc tgtcgccttg atctgcaaca aactggcgaa acgcggccat
    42001 ctgctcatta tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg tcatttttag
    42061 ggcggtaacc gtggagtaat cgacccagtc ggcatcccgc gcctgttgca gcgtctgttg
    42121 cgtggtcggc aactgccacc agacctgagc ctcttcgcta agatggaaat cttcgacggc
    42181 gttaacgtcg atataaatca cattcagcca gcggcgagaa gacgggctgt atgggctggc
    42241 gctctccgga tttgccggat agagcgcatg aatcgggttc agtccgatga acgacccgcc
    42301 acgttttgcc acatccacca gcatcgcttt gagatcgcca aaatccccaa taccccagtt
    42361 tttttccgat cgcagcgtat aaagctggac gcaggcaccc cacagctttt gtttattcag
    42421 caacgcctgc ggttcgtaac agcgtttcgg ggcgacaatc acccggcaat gcgcgcgctg
    42481 gtcgtcctgg gtgagtgtca gcgtgtgata accttccggc agcttcgtcg gtagattgaa
    42541 cgctttgccc cccgttacat ggcctttgta ctgtgttcct tcttcggtgg tcagcagcca
    42601 gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg tataaaccat
    42661 gacattcggg actggcgtta ccgccacttt cgtggcggta cgttgatgca tcgcgtcaag
    42721 caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat tgatgtaatt
    42781 ggggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca tcgagcttcc
    42841 ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat ccgagctaaa
    42901 cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct ggtcgcggta
    42961 cagcacatcc acctgctttt gtgcctctac ataggctgcg aaatccgcca tcaccagata
    43021 cggatcgccg ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat gcttatcgcc
    43081 gtcgctgtat ttgccgcttt ccagctcttt caatactgcg tccagcacct tatctttctt
    43141 ccgccatttc accgggtcgt agcctttggc cagaattgcc ttcacttgtt ccacggtatg
    43201 accaaaaata aagatatttt cttcaccgac tttctcggcg atttcaacgt tcgccccatc
    43261 cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg taccggaagc
    43321 ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccggaatca gtttttccgc
    43381 cgccgaaacg caataatccg gcaggaacac caccttcaac ttatcgccaa ccagcggatc
    43441 gttgttgatc acgtcagcca ctttgttgat cgcaaagata atattcttcg ccaggtagta
    43501 gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt acgcgatcag cctgcgggtt
    43561 ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcaggtgct ggcgtttgta
    43621 ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct caataccggt
    43681 acgaactttc acaaactccg ccagacggac tttattcgcc tgcttgatct cgcgatattg
    43741 ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagctgat cgagatcgtt
    43801 agcccactct tttttcagtg atttatccaa cagagccgcc agtgccgggt tgcactgttt
    43861 gatccagcga cgtggggtaa taccgttggt gacgttatgg aatttgttcg gccatagctg
    43921 gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg caacaccgtt
    43981 caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttgt cgtgcaccac
    44041 cgccagtttg gcccacactt tttcatcgcc cggccaggtt ttctccacca gcgttttaaa
    44101 gcgagtatta atttcgttaa taatctgcat gtggcgcggc agtaagcctt tcaccagttt
    44161 cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag cgaaagtttt
    44221 gctggtaatg gcccaggcgt catcccagct catctggtgc tcatcgatca gcacgcgcag
    44281 cagttctgga atcgcgatag ttgggtgggt atcgttcagc tgaataactt cgtaatccgc
    44341 cagttcgtgc agtttacgcc ccgcctgatg atggcgacgc aaaatatccg ctaccgaaca
    44401 ggcacactgg aagtactgct gcatcaagcg cagcttttta ccggcagtat ggttgtcgtt
    44461 tggatagaga actttggtca gtttttccgc attgatgccc tgctgttcgg cacgcaggaa
    44521 atcaccgtcg ttaaatttag tcagatcaaa cggatgcgcg tgcgtcgcct gccacagacg
    44581 cagcggctgc gccacgccat tacgataacc gacaacgggg agatcccacg cttgaccggt
    44641 aatggtaaac tccggctccc agcgtccgtc tttcgtcact ttaccgccaa tccctacctg
    44701 cacatccagt gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc agtcatccgg
    44761 cgcttcaacc tgtttgccat ccacaaaaga ctggcggaac aaaccatatt gatagttcag
    44821 accgtaaccc gtcgctgact gaccgacagt tgccattgag tcgaggaagc acgccgccag
    44881 acgtcccaga ccaccgttac ccagcgccgg gtcgatctct tcttccagca ggtccgtcag
    44941 gttgatgtca taagccttca acgaatcctg tacctcctga taccagccga gattcaacag
    45001 gttgttgccc gtcagacgac caatcaaaaa ctccattgag atgtagttaa catgtcgctg
    45061 attcgccacc ggtttggcga atggctgagc acgcagcatt tcggccagtg cttcactcac
    45121 tgccagccac cactggcgag gagtcatttc agccgcagaa tttaagccat aacgctgcca
    45181 ctgacgtgaa agcgcttcct gaaattgctt atcgttaaaa ataggttgtg acataggagt
    45241 tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct cgatgttgac
    45301 cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca aggaaatgtg
    45361 atctcaacca cttaaagcta gtgcaaacca caggattagc agcaaatcaa tgccatacag
    45421 cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa aaagcggaag
    45481 gtaactctat aaattaagta aaggagtgaa acagtttcac aagtaaaata tccagtgtgc
    45541 tccatctcat tcttaataga tttattaaga tcatcttttt agatagcact ttcatcagga
    45601 atgaagaaga aacccttgct taaatgaatc tgatgaacat aagggaaacc agtattcacg
    45661 ctggatcagc gtcgttttag gtgagttgtt aataaagatt tggaattgtg acacagtgca
    45721 aattcagaca cataaaaaaa cgtcatcgct tgcattagaa aggtttctgg ccgaccttat
    45781 aaccattaat tacgaagcgc aaaaaaaata atatttcctc attttccaca gtgaagtgat
    45841 taactatgct gattccgtca aaattaagtc gtccggttcg actcgaccat accgtggttc
    45901 gtgagcgcct gctggctaaa ctttccggcg cgaacaactt ccggctggcg ctgatcacaa
    45961 gtcctgcggg ctacggtaag accacgctca tttcccagtg ggcggcaggc aaaaacgata
    46021 tcggctggta ctcgctggac gaaggtgata accaacaaga gcgtttcgcc agctatctca
    46081 ttgccgccgt gcaacaggca accaacggtc actgtgcgat atgtgagacg atggcgcaaa
    46141 aacggcaata tgccagcctg acgtcactct tcgcccagct tttcattgag ctggcggaat
    46201 ggcatagccc actttatctg gtcatcgatg actatcatct gatcactaat ccagtgatcc
    46261 acgagtcaat gcgcttcttt attcgccatc aaccagaaaa tctcaccctg gtggtgttgt
    46321 cacgcaacct tccgcaactg ggcattgcca atctgcgtgt tcgtgatcaa ctgctggaaa
    46381 ttggcagtca gcaactggca tttacccatc aggaagcgaa gcagtttttt gattgccgtc
    46441 tgtcatcgcc gattgaagcc gcagaaagca gtcggatttg cgatgacgtt tccggttggg
    46501 cgacggcact acagctaatc gccctctccg cccggcagaa tacccactca gcccataagt
    46561 cggcacgccg cctggcggga atcaatgcca gccatctttc ggattatctg gtcgatgagg
    46621 ttttggataa cgtcgatctc gcaacgcgcc attttctgtt gaaaagcgcc attttgcgct
    46681 caatgaacga tgcactcatc acccgtgtga ccggcgaaga aaacgggcaa atgcgcctcg
    46741 aagagattga gcgtcagggg ctgtttttac agcggatgga tgataccggc gagtggttct
    46801 gctatcaccc gctgtttggt aacttcttgc gccagcgctg ccagtgggaa ctggcggcgg
    46861 agctgccgga aatccaccgt gccgccgcag aaagctggat ggcccaggga tttcccagcg
    46921 aagcgattca tcatgcgctg gcggcaggcg atgcgctgat gctgcgcgat attctgctta
    46981 atcacgcctg gagtctgttc aaccatagcg aactgtcgct gctggaagag tcccttaagg
    47041 ccctgccgtg ggacagcttg ctggaaaatc cgcagttggt gttattgcag gcgtggctga
    47101 tgcaaagcca acatcgctac ggcgaagtta acaccctgct agcccgtgct gaacatgaaa
    47161 tcaaggacat cagagaaggc accatgcacg cagaatttaa cgctctgcgc gcccaggtgg
    47221 cgattaacga tggtaatccg gatgaagcgg aacggctggc aaaactggca ctggaagagc
    47281 tgccgccggg ctggttctat agccgcattg tggcaacctc ggtgctgggt gaagtgctgc
    47341 actgcaaagg cgaattgacc cgctcactgg cgctaatgca gcaaaccgaa cagatggcac
    47401 gccagcacga cgtctggcac tacgccttat ggagtttaat tcagcaaagt gaaattctgt
    47461 ttgcccaagg gttcctgcaa accgcgtggg aaacgcagga aaaagcgttc cagctgatca
    47521 acgagcagca tctggaacag ttgccgatgc atgagtttct ggtgcgcatc cgagcacaac
    47581 tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc ggcgcgtagc gggattgaag
    47641 tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct ggcaatgttg attcaatgct
    47701 cgctggcccg tggtgattta gataacgccc gtagccagct gaaccgtctg gaaaacctgc
    47761 tggggaatgg caaatatcac agcgactgga tctctaacgc caacaaagtc cgggtgattt
    47821 actggcaaat gaccggcgat aaagccgccg ctgccaactg gttgcgtcat acggctaaac
    47881 cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg caacattgcc cgtgcacaaa
    47941 tcttgctggg cgagtttgaa cctgcggaaa ttgtcctcga agaactcaat gaaaatgccc
    48001 gcagtttgcg gttgatgagc gatctcaacc gtaacctgtt gctgcttaat caactgtact
    48061 ggcaggccgg acgtaaaagt gacgcccagc gtgtgttgct ggacgcatta aaactggcga
    48121 atcgcaccgg atttatcagt cattttgtca tcgaaggcga agcgatggcg caacaactgc
    48181 gccagctgat tcaactcaat acgctgccgg aactggaaca gcatcgcgca cagcgtattc
    48241 tgcgagaaat caatcaacat catcggcata aattcgccca tttcgatgag aatttcgttg
    48301 aacgtctgct aaatcatcca gaagtgccag aactgatccg caccagcccg ttgacccaac
    48361 gtgaatggca ggtcctgggg ctgatttact ccggttacag caacgaacaa atagcgggtg
    48421 aactggaagt ggcggcaacc accatcaaga cgcatatccg caatctgtac caaaaactcg
    48481 gcgtcgccca ccgtcaggct gccgtgcagc acgcgcagaa actgctgaag atgatgggtt
    48541 atggagtgtg agtttagccg gataatgcat tggatccggc ttacatcttt atatcattca
    48601 atactcaccc gcgttacgcc atccgtttct atcaaaccaa accgcaccgg caagaaacgc
    48661 tccaccaccg cgatattggt cagcagatgg catgagggat gggcgaccgt aaattgtccc
    48721 gcgcccgcca gcgccattgg caacactagc tggtcagcaa gatattcccc cactgccgcc
    48781 gggcttgcca ggtagcgttt cacctctttc accaactgtg ccgcaaccac ctcggcactg
    48841 acgcgctttt caccgacgac aaaaaagcgt tcggtgatat tttcactttc gacttcaagc
    48901 gagacggtat tacccggccc ctggtcgcgc ggcaggttat gaatattctg ctcatgcagg
    48961 gaaaaacttg ccgccagcgt agcgatttca cgctcagcga catgccgcgg cacgccagct
    49021 aataacacct ctccacgcat ctgcacaatg ttcccgcgct cgccaagttg caaggtttta
    49081 aacgatgcca ctggcgagac ttctgttgcc accacgccgc ctccggcagg ataaaaaccg
    49141 tgacgtaaca gcgtggtttg ctgatgaatt ccaattttcg ccagcagcgg ctccagcacc
    49201 cggcggataa aatccgcagg tggggccgac gggttatcgg tgcctccgct cacttcaaca
    49261 cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg tttgcagcac cagcgtacaa
    49321 cttcctgcgc taccgatagc aaagcggtaa tcgccgccgc gcacggtgcc aggccggaag
    49381 accagacgct gcgaccccag ctccgcgcct tccaccgttg ccctacaaat ttccgcagct
    49441 gcttttaccg cggtcagatg ctggcgcaac agtcccggtt ttgcccgccc ggcacgaatg
    49501 ccggtgatgg taaatggctg gccggttatc atcgacagac tcagcgccga gcgcaggatc
    49561 tgcccgccac cttcgccctg tgcgccatcc agcgcaatca tccttttcat catttatcct
    49621 tttacgcaca ccacctgacg cagggtatag ataacttcca ccagatcgct ttgtgccgcc
    49681 atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc ggcatcttta
    49741 cggcattcca catgcgcggt ggcacgaatt tgatcttcca cgctgaacag ttttttcgct
    49801 ttagtacggc tcataacccg cccggcaccg tggctgcacg aacagaacga ctcttcattt
    49861 cccagcccac ggacgataaa gcttttcgct cccatcgacc ctggaataat tccatattga
    49921 ccagcacgcg cagacaccgc gcctttacgc gtcacgtaga tctcttcacc aaagtgctgt
    49981 tctttttgca catagttgtg gtgacagttg atcttttcca tcgccagcgt ttgtggctgt
    50041 ctgaccgttt tctgcgtaat gctctgcaac gccgttacta cgttttccat catcgcatcg
    50101 cggttaaggc tggcaaaaag ctgcgcccag gccacggctt tcaggtaatc atcaaagtat
    50161 tccgtacctt ccataaagta cgccagatca cgcgacggca atgtttcaag cgtttcctgc
    50221 atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat tccgcgtgaa
    50281 ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc aataaagtgg
    50341 ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa acgcggatat
    50401 ttttgcgtta accactgata accggcttca agctcagccc atttcgcatc gacgttaaca
    50461 ggcgggtttt cccatgcgcc tttatcacgt ttacaacggc cagtggtacg cccgtgcggc
    50521 accgcagttt caattgcctg acgcagctcc gccaggtttt caggcagatc tgccgccgtt
    50581 aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc cgccggaata
    50641 atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag gtgtacatca
    50701 ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat aagttgctga
    50761 cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc attttcagtg
    50821 gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt ggtatgtcct gataattgca
    50881 accgtcgtgc cagaaaatta aataagcaga ttaatttttt aattcattgt tttaaaaaag
    50941 attatatctt tacgtccgta accggagatt tcccgcaaag ccgatttacc gataatgaaa
    51001 tatcgtcttt tataaggata tctaagatgc gtaaaacagt ggcttttggc tttgtcggca
    51061 ccgtactgga ttatgccgga cgcggcagtc agcgctggtc gaaatggcgt ccgtcactct
    51121 gtatatgcca gcaagaatcg ttagtcatcg atcgactgga attattgcac gacgcccgct
    51181 cgcgctcgct gtttgaaacg cttaaacgcg atatcgtcag cgtttcgcca gaaacagaag
    51241 tggtgagcgt tgagattgag ctgcataacc cgtgggattt cgaagaggtt tacgcctgcc
    51301 tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat ttaattcaca
    51361 tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggctgaa gcgcgttacc
    51421 tgcccgcccg gttgatacaa tcttcacctc cgcgcaaaaa agaacagccg cgcggcgcag
    51481 gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc agccgctttg
    51541 ccgaggaacg ccagcaaacg cttgattttc ttaagtccgg tattgccacg cgtaaccccc
    51601 acttcaaccg catgattgag cagattgaaa aagtggcgat taaatcccgc gcaccgatcc
    51661 tgctaaacgg cccaaccggt gcgggcaagt catttctggc gcgacgtata tttgaattaa
    51721 aacaggcgcg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgca accctgcgcg
    51781 gcgataccgc catgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc ggggcgcggg
    51841 aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt gatgagattg
    51901 gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc cattgaagaa aaaacctttt
    51961 atccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc ggaacggtgc
    52021 gtgatttgcg ccagctggtt gccgatggca aattccgcga agatctatac gcgcggatca
    52081 atctctggac cttcaccctg ccggggctac gccagcgtca ggaagatatt gaacctaacc
    52141 tggattatga agtggagcgc cacgcttcac tcaccggcga cagcgtgcgt tttaacaccg
    52201 aagcgcggcg cgcctggctg gcctttgcga catctcccca ggcaacatgg cgcggtaact
    52261 ttcgcgagct ttctgccagc gtcacgcgga tggctacctt tgccactagc ggacgcatca
    52321 ctctggacgt ggttgaagat gagataaacc gtctgcgcta taactggcag gagagccgcc
    52381 cctccgcgct tacgcagcta ctgggcgcag aggcagaaaa catcgatctc ttcgaccgta
    52441 tgcaactgga acacgttatc gcaatctgcc gccaggcaaa gtcgctttcc gcagccggac
    52501 gccagctttt tgacgtttcg cgccagggca aagccagcgt caatgacgcg gatcggctac
    52561 gcaaatatct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag cacagctcca
    52621 gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg gcgtcggtgt
    52681 agacggcatc taccatgctg atgctgccca tattgaccat cgcgttacgg ccaaatttcg
    52741 agtgatcgac aaccagcata acgtggcgcg agttctcaat aatggcgcgt ttggtgcgaa
    52801 cttcgtggta atcgaactcc agcagcgagc cgtcgctatc gatgccgctt atccccagaa
    52861 tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca atgatcccgc
    52921 catcgcggct gcgtaattcg ccaccggcga gaataatgcg gaaatcttct tttaccatca
    52981 acgtgttagc gacgttgaga ttgttggtga caatacgcaa attactgtga ttaagcagcg
    53041 cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag ccgttgggga
    53101 tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg gctttacgat
    53161 cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc accgccatga tggcgcagga
    53221 tcaggttttg ctctgccagt tcgttgaggt cgcggcgaat agtctgcggg ctgacggaga
    53281 aatgctctac cagctcttcg gtactgacat aaccctgctg tttaaccagt tcgataatac
    53341 cgttgtgacg ttgtgtttgt ttcatttcta aatccctgga attattttcg ttttcgcgca
    53401 ttgagcgaat caacaaaagc catcgctaaa cccacggcta acccggcgat gtgtgctccg
    53461 ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat cagcgcaaag
    53521 ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc acgtagccag
    53581 acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg cccgctgaat
    53641 ttttgctgca catagccgct taacagggcg ctgataagcg taatgacaat tagcttaccg
    53701 ctaccgaggc gtttttccac cgcaccgccg agataccacc accagagcag gttaaagagg
    53761 atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa ctcaaatttc
    53821 agtgctggat cgaatggcca ggccagccat aacatcactt cctgatcgcc gagaatttgc
    53881 atggcaataa acaccaccac gcaggcgatc atcatcaccc aggttaccgg acctgcgcgt
    53941 tcacgcaagg cggcaaagaa aggataacgg cgataatgca ggccactgcc ggtatggcct
    54001 gcctgccagc tcgccgccag ataacgcgga tctgccgggt tttcgagaaa acgcgccagc
    54061 tccgcccgta cgcgctcggc ctgggactca tccgccagcc agacatcgct ttggttatgt
    54121 tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc ctgcgccacg
    54181 cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc cacacaaaag
    54241 gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactga cgttgccagg
    54301 cttcaaagcc gccatcaatg ctatagacca catcgtagcc ctgttgcagc agatactgcg
    54361 ccgcgccttt gctgctatta ccgtggtaac acatcaccat caccggtgtg tcaaagtcgt
    54421 tatcacgcat aaaagcgccc agcgtgtcgt tggttaaatg gaaagcctgc accgcatgtc
    54481 ccattgcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt tcctgcaact
    54541 tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc tctttcttta
    54601 caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa acgccataat
    54661 gttatacgta tcactctaaa atgttttttc aatgttacct aaagcgcgat tctttgctaa
    54721 tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca gcatggaaac
    54781 caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg cagacgccgc
    54841 cgggcgcggt ttatctgtgc tgatgctgga ggcgcaggat ctcgcttgtg cgacctcttc
    54901 cgccagttca aaactcattc acggtggctt gcgctacctt gagcactatg aattccgcct
    54961 ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggccccgc atatcgcctt
    55021 cccgatgcgt tttcgcctgc cgcatcgtcc gcatctgcgc ccggcgtgga tgattcgtat
    55081 tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat caaccggttt
    55141 gcgttttggc gcaaattcag tgttaaaacc ggaaatcacg cgcggattcg aatattccga
    55201 ctgttgggta gacgacgccc gtctggtact cgccaacgct cagatggtgg tgcgtaaagg
    55261 cggcgaagtg cttacccgca ctcgcgccac ctctgctcgc cgcgaaaacg gcttgtggat
    55321 tgtggaagcg gaagatatcg acaccggcaa aaaataccgc tggcaggcgc gcggcctggt
    55381 gaacgccacc ggcccgtggg tgaaacagtt cttcgacgac gggatgcatc tgccttcgcc
    55441 ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgtgtgc atacccagaa
    55501 gcaagcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc cgtggatgga
    55561 tgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggcgatccga aagcggtgaa
    55621 gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact ttaaaaagca
    55681 gttaagccgt gacgatatcg tctggactta ctccggtgtg cgtccgctgt gtgatgatga
    55741 gtccgactcg ccgcaggcca ttacccgtga ttacaccctt gatatccatg atgaaaacgg
    55801 caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgca aactggcgga
    55861 gcacgcgctg gaaaaactaa cgccgtatta tcagggcatt ggcccggcat ggacgaaaga
    55921 gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattatgccg ctcgcctgcg
    55981 ccgccgttat ccgttcctga ccgaatcgct ggcacgtcat tacgcgcgta cttatggcag
    56041 caacagcgag ctgctgctcg gcaatgcggg agcggtaagc gaccttgggg aggatttcgg
    56101 tcatgagttc tacgaagcgg agctgaaata tctggtggat cacgaatggg tccgccgcgc
    56161 cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg atcaacaatc
    56221 tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg cgtcgtaaat
    56281 taacgtaagg tgatcaggtc agatttcaat ctggcctgag actgatgaca aacacaaaat
    56341 tgcctgatgc gctacgctta tcaggcctac gtggtttatg caatatgttg aatttgcatg
    56401 attttgtagg cctgataagg cgttcacgcc gcatcaggca tgaacaaagc gccacgttgt
    56461 caacaatctg acctactttt tgattcaggg aattaccgca aagcccacgc catcataccc
    56521 aacgtaacaa aactcagaca cgttccccac tcactccact tccgtatctg gcttctattt
    56581 ttgctacgcc actggcaata atatcccaac gcaaaaccaa gctgtgcact tatcggaccg
    56641 acaaagtcga tccactgttg agaccaaaat ggcgaaaaat tcagtgggag ccagacaact
    56701 gtcagaaaga aactgcgcat attttccagc aaagcaattt gcgctggcgc gacataaaaa
    56761 atgatattcc acaggagcaa ccagaaataa actggtgcaa ataccagcca acaatgcagt
    56821 ctgcttttca attcaggtgg cgttctgcgc acaaattgcc aaccaacgcc caccattgca
    56881 cagaagaaaa gcgcatattc agccgataac catagttgta attgcagaag ggaaatacgc
    56941 ctgtgcatga gcgggattga gaacagtatt tcccccagaa aactcagcgt gataatacaa
    57001 gcagccagcg caaccaccgt cggccacacc aacaacggca caataacaaa gccgagaaat
    57061 aacggataaa tataaagtct gattatattc atcattattg tcctgatgat tcgggggatg
    57121 cttcatacag cggcacatac cccacatcct ctaccaactg ttgcccctgc ggacttaaaa
    57181 accagtcaac aaatttctgc gtttccggcg tgggattttc acgcgtcacc atataggcat
    57241 ccacaatgta tggataagtg ccgttgcgga tattttcctc ggtaggcgca acgtcattta
    57301 tagccaaaag tttaatctgc ttgcgcattt tgagcatatt gtcattcata cgagtgacgt
    57361 aatagtggaa ggtgtaccca atggaagatg gcgtgttttg atagtcggca acttcagtaa
    57421 ttaaaccgcc cataagatca atcacagtgg attttttagc tggcagcatt ggggtgtctt
    57481 gcataaccaa ccccttcata atcgtttggc ttccagaatc ttcagggcgc tgccagactt
    57541 ttatatgttc gtcactacca cctactttat tccagcggct agttttgccg ctaaaaatat
    57601 ctttaacctg gtgctcagag agagaattta ccgggttatt aatatcaacg ataaagacaa
    57661 aggcttcgcg ggcgaaagcg gtatatttca gtttaacacc cgcttcttct gcacgttttt
    57721 tctgtccttc cgacggttgt gcaacaaaaa taaccgtcgc ggaaccttta atcagactat
    57781 cgtaggcttc agctgttcgg gtttgctgca aaccatacgc ctgccaggcc agcgaggttc
    57841 gatccttatc ttttggctcg ggtactaatt tataatacgc ggaggcatac agcgggtata
    57901 aggctgtagc gccgtacact ctcggccatc ttgaccaatc atcgctgtaa cgtaatgcag
    57961 gttgcccccg cagagacgtc agtccatggc tctcttcttc cctgacgctg ttatagtacc
    58021 cttcccggtc gacacatccc gccagtaacg ccgtgcctaa tagtaaaaca ccggtgaatt
    58081 tattcatctc gctgattcct ttctctcgag cgttattcca tggttggata cagcggcaca
    58141 tatcccacat cttctaccag actctgcccc tgcggcgtta aaaaccattc gaccagtttt
    58201 tgtgtttctg acgttttatt ttctctcgtc accataaatg catcgacgat gtacgcatat
    58261 ttgccgttgc gaatgttttc cgccgtcggt gtaataccgt taatcgctag caattttata
    58321 tttttatcag cattcatttg cgtcgcgtaa tagcggaagg tatagcctat tgcgttgttt
    58381 gtattacggt attcggcaac gactttaatc attccctcca tcacgcttgc cacttccgtt
    58441 tcctgcggcg agatcatgcg gacatttttc atgacctgtg attgcatcac tgtctggctg
    58501 ccagagtctt ccgggcgctg ccaggtctgg atctcctgat cgttaccgcc aaaagtacgc
    58561 cagttggtaa ttgcaccact gaaaatgtca cgcacctgtt gttcagtcag ggaattaacc
    58621 ggattatccg cattgacgat gaaaacgaat gcttcacggg caaagggggt gtacagcaaa
    58681 gtgacaccag attcctttgc acgttttttt tgcccaccgg aaggttgcgc cacgaaaata
    58741 atatcagcat cgcccttaac gattttgtta tatgcttctg gcgtacgaga gttttccaga
    58801 taatcccaaa cgtgaaaatc ctctggtatt acacttaatg cataaaatgc agaagcataa
    58861 attgggtacg cagccgtggc gccatcgatt cgcggccagt tttgcctaaa ctgaatttgc
    58921 ggtttgccac gcagcggcgt cagtcgatta ttgagtttat cgggtcgcca ggccctgata
    58981 tcaatatttt cgcgtacacc gacaccagga ttttgctgct tatataaatg ggcctgatac
    59041 ccggcgacaa taccaagtaa gagaagtaga aacaggagta agttacggca acgtagtccg
    59101 gacgtgccgg ataaaggata tctggtcggc caggcgtatc ccagcgaaaa actgatatgc
    59161 gccccaacgg gaataatgac catccaccag tactcaccac ccactaatga gactaaaaaa
    59221 acgcccaacc agggaagcat aaggtttgcc aaccaaccag ctcccatctg gcgaaattga
    59281 taatctgcca gatgtagcgt gattatccag ccaaccaggg ttaatagcgc gggtaagaga
    59341 aaaaagcatt gccgccagac ggctatttct ctgttttctg gcgcgctatc accggcccac
    59401 acgcgaccag agaaaaatat tattatcgcc cccagcgtca gcataaagac gttaagtaga
    59461 agataaattg ttggcgtgat aaggatatta caaacttcaa taataccggt aagcccgaca
    59521 cccagcatgg caacaaccgc tgccaaaaat tgcgccagta tggggatgcc gaaaaaagtc
    59581 attatcagcg aggtcaaaat ccattttcta tttggcatca ttctgtccat tcttcctgaa
    59641 tgacggaatt atactccccg agaaccctcg ccccctctgg acacttttcc gaaataaagg
    59701 cgaaaaaaaa cgggaccttg tggccccgtt ctgtttttta gcgaacttac aatctcactg
    59761 gatcaatatg ccagatatga tcagcgtact ctttgatagt acggtcagaa gagaagtagc
    59821 ccatattggc aatgttcagc atcgcttttg cggtccactc ttcctgaagc tcgtagagtt
    59881 catcgacttt atcctgacaa tcgacatagc tgcgataatc cgccagtacc tggtagtgat
    59941 cgccgaagtt gatcagcgaa tcaaccagat cgcgatagcg acccggatct tccggactga
    60001 atacaccgct gccgatttgc gtcagcacct gatgcagctc ctcatctttc tcgtagtatt
    60061 cacgcggttt gtagccctga cgacgcagtt cttccacttc ttccgctgtg ttaccaaaaa
    60121 taaagatatt gtcagcaccg acatgatcca gcatctcgac attcgcaccg tccaacgtac
    60181 cgatagtcag cgcaccgtta agcgcaaact tcatgttact ggtgccggaa gcttccgtcc
    60241 ctgccagcga aatctgttca gacagatctg ccgccggaat gatcaactgc gccaggctaa
    60301 cgctgtagtt cgggatgaac acgactttca gcttatcgcc aatctgcgga tcgttgttga
    60361 tcactttcgc tacgtcattg atcaaatgga taatatgctt cgccatgtaa taggccgaag
    60421 ccgccttacc gccaaaaata ttcacgcgcg gtacccactt cgcatccggg tcggccttga
    60481 tgcggttata gcgggtaatc acatgcaaca cattcatcaa ttgacgtttg tattcgtgaa
    60541 tacgtttgat ttgcacatcg aacaacgcct ttggattcac caccacattc agctgctggg
    60601 cgatatactc cgccagacgc tttttattct ccagcttcgc ctgatgcaca gcgtgattaa
    60661 ccattgggaa atcacagtgt tgttgcagct cattaagcag gctaaggtcg gtgcgccagt
    60721 tacggcccag gtgttcgtcc agcacggctg aaagcgatgg gttcgctacc gccagccagc
    60781 gacgcggcgt cacaccgttg gtgacgttag tgaaacgacc cgggaagatt ttcgcaaagt
    60841 cggcaaacaa cgattgcacc atcagattag agtgcagttc cgatacaccg ttaactttgt
    60901 ggctcacaac aaccgccagc caggccatac gcacacgacg accgttggat tcatcaatga
    60961 tcgacgcccg tcccaacaga tcggtatcgt tcggatactg ttcctgcaag gttttcagga
    61021 aatagtcgtt gatttcaaag atgatctgca ggtgacgcgg cagaatttta cccagcatat
    61081 caaccggcca ggtttccagc gcctcgctca tcagcgtgtg gttagtgtag gagaagacct
    61141 gacaacacac ctcaaacgcg tcgtcccagc taaattggtg ctcatcgatc agcagacgca
    61201 tcatctcagg aatcgacagt accggatggg tatcattgag atgaatcgcg attttatccg
    61261 ccaggttatc gtaggtttta tgcaactgat aatggcggct taaaatgtcc tgaatggtcg
    61321 aggaaaccag gaagtattcc tgacgcaggc gcagctcacg cccggagtag gtggagtcat
    61381 ccggatacag tacgcgagat acgttctcgg agtggttttt atcttccact gccgcgaagt
    61441 agtcaccctg gttgaattta ccgaggttaa tttcgctact ggcttgcgca ctccacaaac
    61501 gcagcgtgtt ggtcgcgtcg gtgtcgtaac cagggattat ctgatcgtaa gcgactccca
    61561 gaatctcttc ggtttcaatc cagcgcgttt ttttaccttc ctgctgaatg cgaccaccaa
    61621 aacggacttt atagcgcgtg ttgtggcgtt tgaattccca cgggttacca tattccagcc
    61681 agtagtcagg cgactctttc tggctaccgt taacgatgtt ctgcttgaac ataccgtagt
    61741 catagcggat gccgtaaccg cgccccggca accctaacgt cgccagagaa tcgaggaagc
    61801 aagccgccag acgtcccagg ccaccgttac cgaggcccgg gtcattttct tcatcaatca
    61861 gctcttcgag atttaacccc atcgcttcca gtgcgccctg tacatcttcg taaattccta
    61921 gcgacaacat ggcgttggag agcgtacggc caatcaaaaa ctccatcgac aggtagtaaa
    61981 cctgacgagt ttcttgcgac aactgggcac ggtttgaacg taaccagcgc tccacgagac
    62041 gatcgcgcac agcaaataac gttgcgttca gccattcatg tttattggcg acgaccgggt
    62101 cctttccaat cgtaaacatc agcttgtaag cgatagagtg cttaagagct tctacgctaa
    62161 gcgtgggcga tgaatatgta aacggagcat tcatataggc gtttcctgaa aactatttca
    62221 agcgatagta aagctcacgg tacgacttcg ccgcgacctg ccagctaaaa tccattgcca
    62281 tagcctgacg ttgcacaaac cgccacagag aaggacggga ccacagtaca aaagcacgtc
    62341 gaatagctcg taacagcgac caggcattac tatcttcaaa gacaaaccca ctggcgacgc
    62401 catctgcaag gttctcaaga gaacagtcag aaaccgtatc agcaagccca ccggtgcgcc
    62461 gcactaacgg cagcgtaccg tacttcaatc cataaagttg cgttaagccg cacggttcaa
    62521 aacggctggg caccagaatg acgtccgcgc cgcccataat gcgatgcgaa aatgcttcgt
    62581 gatagccaat ctgaacgccc acctggccgg ggtattccgc tgccgccgca aggaaacctt
    62641 cctgcagcac cggatcgccc gcgccgagta gcgccagctg cccgccctgc tccagaaggc
    62701 ccggtaaggc ttccagcacc aggtcgagac ctttctggct ggtcagacgg ctcaccactg
    62761 caaaaagcgg cactttatcg tcaaccttaa gccccattgc gatttgtaac tggcgcttat
    62821 tttccgcttt atcttccaac gtatcgcggg tgtaacgcga ggccaacagt aagtccgtct
    62881 ctggactcca gattttctcg tccacgccgt tcagtacgcc agaaagacgc ccttcacgat
    62941 gacgctgttg caacaaacct tccataccgt aggcaaactg cggttcggtg atctcgcgag
    63001 cgtaggttgg actgaccgcc gtaatgtgat cggcatagta cagaccggcc ttcaggaaag
    63061 agatttgtcc gttgaattcc agcccatgaa tattaaagaa tgaccatggc aattggatgt
    63121 cattcatgtg atgtgcataa aacatgcctt gataggccag gttgtgcaca gtaaacaccg
    63181 acttcgccgg acgcccgcgc gccgccagat acgcaggcgc aaggcctgca tgccagtcgt
    63241 gcgcatgcac cacatcagga cgccagaatg ggtcaagccc gctggccatt tctgccccaa
    63301 cccaccccag tagcgcaaaa cgcaatacgt tgtcggtata ggcaaataag ttggtatcgt
    63361 gatacgggct tcccggacga tcatagagat gcggcgcgtc aatcaggtat atgccaaccc
    63421 cgttgtaatg accgaacaac agcgtgatat gcccggcgaa ggtatcacga cgggatacta
    63481 cctgcgcatc ggtcacgcca cggcgaatat cgggaaatgc aggcaacagt acgcgagcgt
    63541 caacgccgtc tgcgatttgt gctgcgggta atgccccaat aacatcagcc agaccgccgg
    63601 ttttaagcag cgggaacatc tctgaacata catgtaaaac ctgcattatc gctcctgttt
    63661 atgccctaac ttccgtagca tttcgcgcgt taccagcacg atgccttctt ctgaacgata
    63721 gaaacgacgt gcatcttcct ctgcgttttc accaatcacc atgccttccg gaataacaca
    63781 agcacgatca atgacgcagc ggcgcagacg gcacgagcga cctacccata cttccggtaa
    63841 caatacggcg gaatcaatgt tgcagaatga attcacgcga acgcgcgaga acagaacgga
    63901 ctgcaccacc accgaaccgg agatcacaca accgccggaa accagtgagt taagggtcat
    63961 cccgtggcta ccggagcgat cctgcacgaa tttcgctggc ggtaatgatt cattgtaggt
    64021 gcgaattggc caattgcgat cgtacatatc cagctccggc accacagagg ccagatcgag
    64081 gttcgctttc cagtaagctt ccagcgtacc cacatcgcgc cagtacggct cggcatccgg
    64141 atcggattgt acgcaagaga gcgggaacgg gtgcgcatag gccagaccgg cgtcggtgat
    64201 cttgggaatc aaatctttgc caaagtcgtg gctggagttc tcatcgcgat cgtcttcttc
    64261 cagcagttca tacagatagt cggcgtcaaa gacgtagata cccatactcg ccagagattt
    64321 gctcggatcg ttcggcattg acggcgggtt agcaggtttt tcaacgaatt cgataatttt
    64381 atcgttctca tcaaccgcca taacgccaaa tgcggaggct tcttcaatcg gtactggcat
    64441 acaagcaacg gtgcaacgtg cgcctttttc gacgtgatcg ataagcatac gcgagtagtc
    64501 ttgcttgtag atatggtcgc ccgccaggat caccacatat tccgctttat aacggcggat
    64561 aatatcgaga ttttgggtga ccgcatctgc ggtgccgcga taccagtttt cccctttcat
    64621 tctctgctgt gctggcagca gatcgacaaa ctcgttcatt tcttcattga agaatgacca
    64681 gccgcgctga atgtgctgca ccagagtgtg ggactggtac tgggtgatca cgcccatacg
    64741 acggatcccg gagttgatgc agttagacag cgcaaagtcg ataatgcgga acttaccgcc
    64801 gaagtgtacg gccggttttg ctcgcttatt ggttaaatcc ttcaggcggg taccacgtcc
    64861 tcccgccagt atcagggcaa cagatttcaa tggcagctgg cgcgccaaca ttaagtgatc
    64921 gttcttctct aaactaacca tgactaactc cttttttatc atctctggaa cacacacaat
    64981 ccgtgtgcag gtccctgcca gacagccgta atcactgggt tatcctctcc agcgaatggg
    65041 ggaatggcgt gccactcccc agcaggtaaa acaatctctg ttacctcaag cgtggcgtta
    65101 attgcgatca aaaagcgatc cgagagcaga atttgcagct gtttcggccc gttttgccac
    65161 tcatccgtgc ttaaaggttg agcatatcga tttagccaac ggacattgcc gtcgccttct
    65221 tcccaccagc gattctccac caaagcggga atacgcttgc gcagatggat taacgcggcg
    65281 gtaaatgcgg ttaaaccact gcttgcctgc gaccagtcca accaggttaa ttggttatcc
    65341 tgacagtagg cattgttatt gccatgctgg ctgtgaccat gttcgtcacc ggccagtaac
    65401 atcggcgtgc cctgggagag caacaacgtt gttaacaggg catgaatgct gtcgcgccgc
    65461 cgttcaacca gatcaagagt accgcctaac ccttctttac catgattgtt actgtaattg
    65521 ttgttggtcc cgtcgcgatt ttcttctccg tttgcttcat tgtgtttatg gttgaagcaa
    65581 acgcagtcgc gaagcgtaaa accgtcatgt gcggtgacga gattaatcgc ggcactcggc
    65641 agacgaccat tacgtttaaa aacatcgctg gaggcagcaa aacgcccggc aaacgccccc
    65701 agaggcaaat cataatgtag ccagaaacga cgggcagcat cgcggaaatg atcgttccac
    65761 tcggcaaaaa gcggcgggaa atttcccacc tgataaccac caggagcgat atcccacggt
    65821 tcagcaatta acttcacctg cgagagcacc gggcagttct ggatagcggt aaacaacggc
    65881 gcatcctgac ggaactctgg cgtacggccc atgactgccg ccagatcaaa gcggaaacca
    65941 tcgacgtggc aggtttctac ccaataacgc aggcaggcgc tggcataatc caccaccgcc
    66001 gggtgactca aattgagcgt gttgccacaa ccggtccagt tgtgataatc gccgtcttct
    66061 cttatccaat aatagctacg gttgtcgatc ccacgcagcg agaataacgg gccgtcgagg
    66121 tccagttccg cactatggtt gagcacgata tcaagaatga cttcgatacc cgctttatgc
    66181 agtgctttga ttgcatcgcg aaactcatgc agcgccgttt ctggcgagca ggcatacgcc
    66241 ggatgcagcg caaacatcgc caccgggttg taaccccagt agttacttag ccccatgcgt
    66301 tgcagacgtg gttcactggc aaactgcgcc accggcagca gttccagcgc ggtaatgccc
    66361 aactgtttca aatagttgat catcaccgga tgcccgaggg ctttataagt gccacggatc
    66421 tcgaccggga tctccgggtg caggtacgtt aatcctttaa catgggcttc ataaatgatg
    66481 gtgctgcccc acggcgtgcg cggcggggca tcatcttccc agtcatagtg atcaaccacc
    66541 actacgcatt tcggcgcaat ggcggcgttg tcgcgatagt caggttcatt atgaccggcg
    66601 tgcagcagcg ggttatcttt aaattccccg tcaatttgcc gcgcgcaagg atcaatcaac
    66661 aacttcgccg ggttaaagcg atgcccctcg gcgggttgcc aggggccatg aacgcgataa
    66721 ccataacgca aacccgggcg cgcatccggc agataaccgt gccaaatgtc gccactgtgc
    66781 cctggcaagt catagcgatg ttcctggcca ttggcgtcaa agacacacag ctctacccgc
    66841 tcggcatgag cggagaaaag tgtgaagttg acgccctgac cgtcgtaatg cgcgccgagg
    66901 ggagcgggct tgccaatggc gagttgtgtc attctgcctc ccgaaccagc cagatagtgg
    66961 ccagcggtgg tagcgtcagg cttagtgaat gctgacgacc gtggctggca atctcatcgc
    67021 tgtgtaccgt gccgccattg cctgcattac tgccgtgata gtgcatggaa tcggtattga
    67081 ggatctcacg ccagttgcct ggctgattaa tgccgaagcg ataatcatga cgcggcaccg
    67141 gcgtaaagtt actggcaacg atgatttcgt taccctcttt atcgcgacgc acaaagatca
    67201 gcaccgagcg ttctttgtca tccaccacca gccattcaaa gccgtacggg tcaaaatcca
    67261 gttcatgcat cgctttatgg tggcggtagg tgaggttcag atcgcgcacc aaacgctgga
    67321 taccgtggtg ccagttatcg ccgccttcca acagatgcca gtcgaggctg gcgtcatggt
    67381 tccactcgcg gccctgggca aattcgttcc ccatgaacag cagtttcttg cccggaaatg
    67441 cccacatcca gccatagtaa gcgcgcaggt tagcgaattt ctgccatgcg tcgcccggca
    67501 tgcggtcgag aatcgatttt ttaccgtgga ccacttcatc atgcgacaac ggcaggacga
    67561 agttttcagt gtagttgtag agcattccga aggtcagttt atcgtgatga tactgacgat
    67621 aaatcgggtc gagcttcatg tagtccaggg tgtcatgcat ccagccgagg ttccacttgt
    67681 accagaagcc cagaccgccc atatcctgcg gacgagaaac gccagggaaa tcggtagact
    67741 cctcagccat tgtcaccgca ccggaaacct gctcaccaag aatacggttg gtattacgca
    67801 agaattcaat cgcttcaaga ttctcgcgcc cgccaaattc gttcgggatc cactccccct
    67861 ctttacggct gtagtcacga taaatcatcg acgccaccgc atcgacgcgc agcgcatcaa
    67921 taccaaaacg ttcaatccag taaagcgcgt taccgacgag gaagttactg acttcacggc
    67981 gaccatagtt gtaaatcagc gtgttccagt cctgatgata gccttcgcgc ggatcgctgt
    68041 gttcatacaa gttcgtacca tcaaattcgg caagcgcaaa gtcatcagtc gggaagtggc
    68101 ctggcaccca gtcgagaatc acgttcagac cagctgcgtg tgcagcatca atgaaataac
    68161 ggaagtcgtc gcgagtacca aaacggcggg ttggtgcata caggccggtt ggctgataac
    68221 cccaactgcc atcgaaggga tgctcgttaa tgggcagtag ttcgaggtgg gtaaagccca
    68281 tccatttagc ataaggcacc agttgatcgg ccagctcgcg gtagctcaac cagaaattgt
    68341 tgtcggtgtg gcgacgccag gagcccaggt gaacttcata aatagagatt ggcgcatcaa
    68401 actgattcgc ttttttgcgc tcttcagtct gtacaacctt ttccggcagc ccgcaaataa
    68461 gagacgcggt ttccgggcgc atttgcgctt cgaaggcata agggtcggac ttcagacgca
    68521 agttgccatt ggcatcaatc atctcgtatt tatagagctg accgttatgc gccccaggga
    68581 taaacagttc ccagatgccg ctctctttac gcaggcgcat cgggtgacgg cgaccgtccc
    68641 agtagttgaa ttgcccaacc accgagaccc gacgggcgtt tggagcccag acagagaaac
    68701 gcgtacctgt gacgccatcc atagtatctg catgcgcgcc taaagtttca tacgggcgca
    68761 ggtgagtacc ttcagataat agccaggcat ccatttcctg gattagcgga ccaaaacggt
    68821 aaggatcatc aatcaggttt tgctgcccat gccagacaac agccaactga tagcggaaaa
    68881 aattcttacg tcgcggaatg acgccactaa agaatccccg tgagtcgaga cactccagtt
    68941 ttgcgagttt gcgcccggtt ttcggttcaa tcacccacac atcggtagcg tcgggtaaaa
    69001 gggcacggac ttccagtccc gcggtggttt tatgcattcc cagtacggaa aaaggatccg
    69061 caaaatggcc tgcaattagc gcgttaatca cgtctctatc gatacgatcg gacatgcttg
    69121 tcttcctgtt ttattgtgtc accccatcca attggatttt tgacttctgg ttgtgacatt
    69181 tttttgacct gaacggcgca gcactctgtg catcctctct gcgtcgtcct cacttcaggt
    69241 aaggctgtga atactaatgt attccgccac ccttaaagaa tagccaatgc tctatttaac
    69301 tcccggtaaa tcatgaaaca tctgcgctta ctcctgtatt acgcactaac aggggcggca
    69361 tcgcgcccca gatttaatga ataaagatta cgccagttga cgaagcatcc gacgcagcgg
    69421 ctccgcggcc ccccacagca gctggtcgcc cacggtaaag gctgacagga actctggtcc
    69481 catattcagc ttacgcaggc ggcctaccgg cgtggtcagc gtgccggtaa cggcagctgg
    69541 ggttagctca cgcatagtga tttcccgatc gttcggaacg actttcgccc acggattgtg
    69601 tgcagccagc agttcttcca cggtcggaat agacacatct tttttcaatt taatagtgaa
    69661 tgcctggctg tggcagcgca atgccccgac acgcacacat aaaccatcta ccggaattac
    69721 ggaagatgtg ttgaggatct tgttggtttc cgcctgccct ttccactctt cgcggctctg
    69781 accgttatcg agctgtttgt cgatccacgg aatcaggcta cccgccagcg gcacgccaaa
    69841 gttatccacc ggcagctcac cgctacgggt taaggttgtg actttgcgtt cgatatcgag
    69901 aatagcagag gacggggtcg cgagttcatc tgctacatgg ccatacagat ggcccatctg
    69961 ggttaataac tcacgcatat gtcgcgcacc accgccggaa gcggcctggt aggttgcaac
    70021 ggacacccaa tcaacaagat cattggcgaa taaaccaccc aacgacatca acatcaggct
    70081 tacggtacag ttaccgccaa caaaagtcct gatgccatta tttaatccgt cggtaatgac
    70141 gtcctgattg acggggtcaa gaatgatgat ggcgtcatct ttcatgcgca gagacgatgc
    70201 tgcgtcaatc cagtaaccct gccatccgct ttcacgaagc tttggataga tttcgttggt
    70261 ataatcgccg ccctgacagg tcacaatgat atcgagggcc tttagcgcct ccagatcaaa
    70321 ggcatcctga agtgtgccag tggttccgcc aaaagacggc gcagcctgac caagctgaga
    70381 agtagaaaaa aagacagggc gaatggcgtc gaagtcgcgc tcttcaacca tgcgttgcat
    70441 gagaacggag ccgaccatac cgcgccagcc gataaaacca acatttttca taagcgtttt
    70501 ttcctgcaaa gatgtgtgct gtataaatgt gccggtctcc tcttggcaca tctttcacca
    70561 tacaaaaagc agccaaagtc gcaagtgaaa ttaatcaatg atagcgaagc catcagtaat
    70621 gcgacttatc ctgctttgtt agcacgcaga aagtccgcgg caattatcag ggaatttgag
    70681 ttatgaatga aatcatttct gcagcagttt tattgatcct gattatggat ccgctcggaa
    70741 acctacctat tttcatgtcc gtactgaaac atactgaacc gaaaagacgg cgggcaatca
    70801 tggtgcgaga gttgcttatt gctctcctgg tgatgctggt gttcctgttt gcgggtgaga
    70861 aaattctggc atttctcagc ctgcgagcag aaaccgtctc catttctggc ggcatcattc
    70921 tgtttctaat tgccattaag atgattttcc ccagcgcttc aggaaatagc agcgggcttc
    70981 cggcaggtga agagccattt atcgtgccgc tggcaattcc gctggtcgcc gggccaacta
    71041 ttctcgccac gctgatgttg ttgtctcatc agtacccgaa tcagatggga catctggtga
    71101 ttgctctgct gctggcctgg ggcggcacct ttgtcatcct gctacagtct tcgctatttt
    71161 tacgtctgct gggcgagaaa ggggtgaacg cacttgaacg cctgatggga ttgattctgg
    71221 tgatgatggc aacccagatg ttcctcgacg gcattcgaat gtggatgaag gggtaaggtt
    71281 caaacaggtg ctgcacacaa ccagacgcgg cacctgtttt attacctgaa ctgataatga
    71341 ccttcgccca atcggataac ccttccctct tcttctgcgc ggttcagcca cacactaatt
    71401 tgttcgatgg ttaaaccagt acaagacgcc agcctttcaa tacttatcga ttcctttgca
    71461 aggatcgcca gttccgccac aaagagttga taaaaatcga caggcgtata cgcgggcatt
    71521 actgcgtaat ttgcgtcatc ttcatgcagc gaaaacagtt ctggttgagc gttacggagt
    71581 gctctgtcat acatcagccc aggaacgtct ggcagtctga tcgtcacatt ctcagcctgt
    71641 tcagcactcc atgacgcccc attttctact aatcgtgaat tggctgattg catatcctga
    71701 tttggtttaa cctgcacagg cagccactta tgttttaatg cctccatcgc cccggttatc
    71761 gttccaccgg tcattcccgc acgtacaacc attgcgcttt cagcaaggca ataaatatat
    71821 ttatttcgcg ccatcgcatt gccgacggtg aaacgtacct ctgggtaaaa cggcgaaatc
    71881 aacaccaggt tgcctgctat aagcccttca cgccatttca ctaacgtact cgtttttaac
    71941 aagctatcag ctaatacgcc aacggcagtt cccccggcct ccagtgccga cgccattgca
    72001 cattcatcaa tacctcgcgc accaccagag ataacgcaaa tcccctgttg ggccagtttc
    72061 gcggccagtt gttgggtata gcgcaaatcg tcagtcggag catctcgcga gccaacaatc
    72121 gccatacctt ccgcttgcag taatgcttta ttcccgcagc caaataaaac gggaggtgca
    72181 tccgttcgca atcggttttt caagcgaacg ggataatcag catctccgcg ggttaagatc
    72241 cataaaccgg cgcgttgcca cttatctacc gccaacgcca gactatgacc acgcgccagc
    72301 aagccaagaa tacgctcctg agaaatacgc ggatcgctcc agcctgtaag tttctcttgc
    72361 ggttgcggca ccagaagctc ggcgggactg atacgttggt gcttcagcca taatgcaaac
    72421 ttcccccatt cactattact aagaggttta tactcactcg ccgccgcgcg agaaaaatcg
    72481 ctggttagca ggagagttgc ttgtgcattg gctgaaagat tcatcaattt tttaccgagg
    72541 tagaagcaag ggcaagagga taaaccgtcg ggcaacctgc ctggcgtaac agtgctgtca
    72601 gaactgtcag cgtccatgca gaatccacga tatcgtcaac cagcaacgcc ggaccaggca
    72661 tcaaaggagg ggtaatcaca aacgccccgt cgagattttg acactggtgg aaacggtttt
    72721 gctgcatttt ctgcggtgga ttgtccacga ctttttcaat ggcatcaata aaaggtaagc
    72781 caagtttcgc cgccagtcgc cgggcaaaat caggaaccag gtcgaggtgc tttaatgaag
    72841 gaacgcagca tacccaggtt ggttcaggat gcggattcca gcgttggcga accatttccg
    72901 cacatgcctc caccagttca tcactgaatc gccccgtttt tttctcctgt gctacctgct
    72961 ttccccaccc ggaatctccc caacgagaaa ggattcgccc cgtagatcct tgtaaaatcg
    73021 caggcaagtt ccctttaaat ccgtattgcg taaaagcccc agctgccacc tgtttattga
    73081 gatttaatga caggtcagcg tgtttaataa ataagtttgc cgcatggagt aagccgctat
    73141 caatgtcggg cgacaacaac ggatgctgaa gacaactgct acatttacca caatgttcgg
    73201 cacctggctc atcgagtgcg cggcgcagaa attgcatttt gcactccttg gtcgtgatat
    73261 agctttcaac gtctgccagc tcactttttc tctgattcat caaatggtta attcgttcat
    73321 ggtcaggaga aaaactgaca attgttctgc gccataattt ctcggtatac accacaggcg
    73381 atggattttc agctaccaat aatttcagtg ctttttctat ttgcccataa cggagattcg
    73441 tccgttgttc aatgcctcgt aaggtaagac cgtcattcac gctaagtacg ttgagtattt
    73501 catgaatttg cgcctccgca ggaaaggcgc tttcacggaa gaatttatga atagcacggt
    73561 cttcactgcc gcaaagcagt atgccaacag cagaatcgat accgcgaccg gcacgcccca
    73621 cctgttggta gtaaccaact atcgaacctg gcatctggta atgaataaca aaccccaaat
    73681 ccggtttgtc aaagcccatt cccagcgccg ttgtcgcaac cagcacttta attttatttg
    73741 ccagcaacgc ctgctccaga tattccctgg cagtattaga atcttccgcg ccttcacaag
    73801 tcacgccgct gtagtaagca aatgcactga taccattttt ccttaaccac tcggcgacaa
    73861 gttcggcatc gcgagtagtc agggtataaa caattcccga tttggaaaac tgagggataa
    73921 ccgttgctaa ccatgccaga cgggatgact gttcacctaa gactaaggca tcaagcgcca
    73981 aactttcgcg agccagcgtt ccacgctgaa tcacaatgtc acccaattgc tgacggatat
    74041 cctcaacgac acggttattc gctgtcgcgg ttgtacccag aacaggggta ttcgcaggta
    74101 gttggcgcaa aatatctaat atacgtcggt aatccgggcg aaaatcgtgg ccccaatcag
    74161 agatgcagtg cgcctcgtcg accacaagca agccaatatg atcggttata ggatataaaa
    74221 ccgtttcgat aaagtcctgg tttgccaaac gttccgggga aaccagcaag caatcgacct
    74281 caccttgcag caacttatcg ctaatgcgct gccattcttc tctgtttgta gaattcaacg
    74341 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
    74401 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
    74461 agtacacagc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgttggt
    74521 tcaccaccgc gtctatggct tcccattgcc cttccctgaa agtagcggtg gtatttccta
    74581 acatccgctg taaaagcaat tcagctgcgt gcttttccat ctttatctcc tgcgatcttt
    74641 aggcagtgat tagaacggat tatttgcagg taaatttgcg agagagatca aaaagatgaa
    74701 gagaggagaa aagagaaatg aattactgcg agcaagctcg cagtaatttg tcgcagtaat
    74761 taactcaaca gctggaacgc aatcatccca acgatggcac cgacagtgcc gaggatggtt
    74821 tccatcatgg tccaggtttt cagcgtttcg gcttcggtcg cgccggtaaa tttaccgaac
    74881 aaccagaaac cagcgtcgtt aacgtggctg acaacaatcg aaccaccggc gatacaaatc
    74941 gacagcgccg ccatttgcgc accggagtag ttcagttgtt caatgaccgg catcaccagc
    75001 cccaccgccg ttaaacaggc aacggtggca gacccctgaa tgatgcgcac tgcagctgcc
    75061 agcacgaagc aggtgatagc aatcggcagg cccatgccgg ttaacgcttc gcccagtgcc
    75121 ggacctacgc cagagtcaac cagcacctgt ttgaacacgc cgcccgcacc aatcaccagc
    75181 agaatgatcc ccgccggttg cagcgcgtga ccgcaaatct ccatcacttt gtcttttggc
    75241 atgccctgac gcattgccag gccataaatc gctaccagac aagcaaccag aatcgcggta
    75301 aacggatgac caataaactc gaaccattcg taagcggtag agccttccgg cacaaaacgc
    75361 gcggcaatgg ttttcagccc caccagcacc agcggcagca ggatcagcga caggctgaat
    75421 ccgaaggatg gcattttgcc ttcgccgaga tgcggttcgc tgatgtcgtc aggaatatgc
    75481 agctcaacgt aacggctgat gaagttaccc cacagcggcc cggcaataat cattcccgga
    75541 attgccgcac acaggccaat caggatcatc cagccaaaat cggcgttcat ctgcgatgcc
    75601 agcagcattg gcgctggccc tggcaccagg aacgccgccg ctgccgccac gcctgcgaat
    75661 aatgggatta ccagcttcac caggttcgta ccggtgtggc gcgccattga gaaagcaacg
    75721 ctaatcagca gaacaatcgc cacttcaaag aacagcggta gcgcacagac cagccccgca
    75781 aggccgatgg cataatgcgc gcggctgtga ccgaaggatt taagcatttt gacggcaatc
    75841 tgatcgactg cgccggtttc atgtaagatc ttgccaaaca tagctcccag ggcgacaacc
    75901 accgccagga agccgagggt gcctcccatc cctttttcca tcgtcgctgc gattttatcg
    75961 agcggcatac cggaaaaaag gccagccccc atggacacca ccattaaagc caggaaagcg
    76021 tgcatacgcg ccttcatgac taaaaacagc agcagtaaaa cagaccctac tgctgttaaa
    76081 acaagcgtta atgtagtcac tacttatttg ccttttttaa taacctcaat ggtgcttgcc
    76141 acaacacctt ccagcggttg atcgatatcc accaccagta catcggtttc gtccgcaccc
    76201 ggctcctgca gcgtttcaaa ctgcgtcacc aacatttggg ttttaaagaa atggcctttg
    76261 cgcgctttca ggcggctttc aatcacatca aaatcgcctt tcaggtagat gaaagagaga
    76321 ttcggattac cttcacgcag caagtcgcga tagtgttttt tcaatgcaga acagacgatc
    76381 agcgacactt tattagtgcg ctgcatagca aacgcggcgt cgttcagcgc ctgcaaccac
    76441 ggtttgcgat cgtcgtcatt cagcggttcg ccagacgcca ttttttcgat attgcgccgt
    76501 ggatggagga aatcgccatc aagaaacgcg gcatgaagtt gatgcgccac ttcacttgcg
    76561 accgcagatt tgccgctgcc cgatacgccc atcaagacgt aaatgtggtg atcatggtta
    76621 gtcgtgctca aagtggtgcc cccacaatac aagaattaaa aatgttacgg gtaactgtta
    76681 tcggtaacat tctccggccg gacaatatca gaagcaatat ccatccgtgc cttaagtgta
    76741 taagtgtgag ctacttcaaa tttgtgggct taaatagatc cgcccggtga caaggtgaaa
    76801 cctaaatcta acattttcgg tgtcacagat tcgccacgaa tacgcgccag aaggcgttca
    76861 gcgccaatac tgcccatccg ctcacgcggc gtcagcacgc tcgcaagtcg tggctccatc
    76921 acctgaccaa tgtcatgacc gtggaaaccg gcaatcgcca tatcgtcagg aacttttaac
    76981 cccagacgct gacattcaaa cgccgcgccg accgccagat catcattagt acagaacacg
    77041 ccatccagct gcggatattc ccgccgcgcc tggcgaatca gttcaatacc ggaagagtaa
    77101 gaagaagatt gctcaaccat cacgctatac ggcaccaggc ctgcatcccg catcgcctgt
    77161 tcgtatccct tctgtttgat gatagtacgt tcgtcgagac gtgcgccgag ataggcaata
    77221 tggcgatgcc cgcgagcaat aatggcggcg gtcatctggc gtgccgcttc aaagttatca
    77281 aaaccgacgg caatatcgag gcatggcgac ttgctgtcca tcagttccac cacgggaatc
    77341 cccgccactt caatcatctt taaggtgcgc ggcgtgtggg tacgttcggt gaggataagt
    77401 ccgtcgatat tccatgagag cattgattcg agacgttctt gctccatttc cggtttataa
    77461 ccgtagtggg ccagcatggt ctgataaccg tgcgcgtcgg tgacgctttc gattccgcgt
    77521 aatacttccg cgaaaacctg gttggtgaga gaaggtaaca ggacgccaat cgcccggctg
    77581 gtggcgttag agaggatatc gggcgcacga ttgggaatat agcccagttc atcaagagcc
    77641 gcggcaatct tgccgcgtag agcgacggaa acctgctccg ggttgcgtaa aaaacggctg
    77701 accgtcattt tggtcacgcc tacacggtca gccacatcct gaagtacggg tcttttcttt
    77761 ttcatcgtcc tgaaggtaca aaagagatag attctcttag tttaacacgg acgcatcaga
    77821 accttcccgg ggaaaacagt gcttgtttac aattattaga tatacactaa atcaattgca
    77881 tcgcattgtg ctaacgacgc ggcaaaaccc cggaagcgta catcagcacg cgaccggagc
    77941 gagcgaggaa gcctgcaaaa aggcaggctt caaagatgcc gttgagtttt aaaccggcgg
    78001 cagatcgaac agtaacactt cgctgtcgct atccgcatgg atggagattg cctgctcatc
    78061 ccagattgcc agaccatcgc tgatcgaggc tttcacgccg ttaatggtga cattgccttt
    78121 caccacctgg atccagacgc ggcgttcagc ggcaatctga tgcaccgact gctcatcttt
    78181 cagcaacgcc cagcggtaca gttccatatc ctgatgcact ttcaatgaac catcacgcgc
    78241 atctggcgaa agcaccagtt gtttgccctg cactgcatcg aagcgacgct gttcataacg
    78301 cggcgtaata ccgttttctt caggcatgat ccagatctga tacagatgca gacgctcggt
    78361 gctgcttggg ttgtactctg agtgacgaat acccgtaccc gcactcataa tctggaactc
    78421 acccgccgga acctgctctt tattgcccat gctgtcctgg tgctcaacag taccttccag
    78481 cacgtaggtc aaaatttcca tatctttatg cgggtgagtg ccgaagccct gccctgcttc
    78541 aatcacgtcg tcgttaatca cgcgcagcgc ggagaagccc ataaagttcg gatcgtaata
    78601 gttggcaaaa gagaaagtat gccaggagtc cagccagcca tgatttgcat gaccacgttc
    78661 atttgctttg cgtaagtaga tcatttgtat tcgccccctg aatgatttcg atgggcttag
    78721 tgtggacccg atctgcctgg gatgatagag ggtgaaaatt gacccctctg ttcaaaaaaa
    78781 ttgaactatt tgaggggcca tcaaacttac ttcgcgagag tcacggtgga gggagaggct
    78841 tgctcaaatc cgcgttcaag gatttccaga ttggtaagaa cttcagattc cttgacgtaa
    78901 tttggcgcac cgtgggtgat ggtttgatac aacgcatcat aaacgcgccc gtaatcgccc
    78961 atctccggct tcatctcttc tctgaccgtc acgccctcgt cattgacata ctccagcaca
    79021 ccgaccgaat catccgctgc gaatcccggt tcgcccggca taatattagc cttcaggctg
    79081 gtttcctgct ggtcgatacc gtatttaata aacgaacctt tcttaccgtg aacgataaat
    79141 ttcggataat cgattttcac cagatggctg gttttgacga tggcttttag atcgccataa
    79201 aacagttgcg cttcgaaagt atcgtcagga ttggctttat tacgcaggct gcggatgtca
    79261 taagcgacgt gatccgggcg accgaacaga gaaataatct ggtccatcgt atgcacacca
    79321 aggccataga acgcgccatc ctgcggcaac ccaggtttgg tttctgcgac cgggcggtaa
    79381 tagtcaaaat ggctttccac ttcgacaatc tctcccagct tgccgctttc aatcgctttt
    79441 ttcgctgtca ggaagcagga atcaaagcga cgattctgat atggcgtgac ggtcagccct
    79501 ttgcttttcg ccagtgcaaa cagctctttc gcctgcgcaa gtgtcggagt gaacggtttt
    79561 tcgaccagca catttttccc ggcttccagc gcgcgtttcg cgtactcgaa atggctgtcc
    79621 gcgtgggtgc agaccacaac cagcttaaca tcgggatcgt ttagcacttc gtcgagatcg
    79681 ctggtgaaat ggatatgaga gtaaatggga gcctgttctt ccggcttcgc atggcgacga
    79741 aaaatatgcg cgacatgcca gctatccttg cggttaagta catacggcag atggtaacgg
    79801 gtggtgcttt tgccgaagcc aataaaggcg cagttgatga ccatgatttc gtccttttta
    79861 aggtggttat ttacacctta gcgcaaagcg gacgtggttc ctactggagt gcgcataacg
    79921 aacacaagca cttcccgagg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
    79981 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
    80041 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
    80101 tgcaaaaaaa atgcatttga ctcgcattcg aaagtcaatg atgttgaaag ggacatttac
    80161 cccaaagagg acaaaggaat gagtgagata gtgatacgcc atgctgaaac gcgcgattac
    80221 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
    80281 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caaacagtta
    80341 gttgcctgta ttgatggaac cgtcgtgggt cacctcacca ttgacgtgca acagcgccca
    80401 cgccgcagtc atgttgccga ttttggtatt tgtgtcgact cccgttggaa gaatcgcggc
    80461 gtcgccagcg cccttatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
    80521 cgtatcgagc taacggtgtt cgtcgataac gcgccagcaa ttaaggtcta taaaaaatac
    80581 ggctttgaaa ttgaagggac tggtaagaag tacgcattac gtaatggtga atatgtcgat
    80641 gcgtattata tggcgcgggt gaagtaatat agtgcccttt ttctgagatg gaaaaagggt
    80701 gtcattcaaa atcggcatac ctt
//
LOCUS       DAZFFG010000008        76135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFFG010000008 DAZFFG010000000
VERSION     DAZFFG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.008"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            25..558
                     /locus_tag="ACHJ7J_000736"
     CDS             25..558
                     /locus_tag="ACHJ7J_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4999011.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            608..1291
                     /locus_tag="ACHJ7J_000737"
     CDS             608..1291
                     /locus_tag="ACHJ7J_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4999012.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1304..3754
                     /locus_tag="ACHJ7J_000738"
     CDS             1304..3754
                     /locus_tag="ACHJ7J_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4999013.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMRGEKVSEYRF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDEAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSHTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGKWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYTIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     VSNFDFIAGRSQIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDTRHEGTSYQVAYNKYLLQTATHFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNNWQHISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSLLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            3767..4726
                     /locus_tag="ACHJ7J_000739"
     CDS             3767..4726
                     /locus_tag="ACHJ7J_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO4999014.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNIGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(4764..5663)
                     /gene="nhaR"
                     /locus_tag="ACHJ7J_000740"
     CDS             complement(4764..5663)
                     /gene="nhaR"
                     /locus_tag="ACHJ7J_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO4999015.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(5729..6895)
                     /gene="nhaA"
                     /locus_tag="ACHJ7J_000741"
     CDS             complement(5729..6895)
                     /gene="nhaA"
                     /locus_tag="ACHJ7J_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO4999016.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            7481..7633
                     /gene="mokC"
                     /locus_tag="ACHJ7J_000742"
     CDS             7481..7633
                     /gene="mokC"
                     /locus_tag="ACHJ7J_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO4999017.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(7737..8867)
                     /gene="dnaJ"
                     /locus_tag="ACHJ7J_000743"
     CDS             complement(7737..8867)
                     /gene="dnaJ"
                     /locus_tag="ACHJ7J_000743"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO4999018.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8956..10872)
                     /gene="dnaK"
                     /locus_tag="ACHJ7J_000744"
     CDS             complement(8956..10872)
                     /gene="dnaK"
                     /locus_tag="ACHJ7J_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO4999019.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            11250..11654
                     /gene="yaaI"
                     /locus_tag="ACHJ7J_000745"
     CDS             11250..11654
                     /gene="yaaI"
                     /locus_tag="ACHJ7J_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO4999020.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            11680..12393
                     /gene="msyB"
                     /locus_tag="ACHJ7J_000746"
     CDS             11680..12393
                     /gene="msyB"
                     /locus_tag="ACHJ7J_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4999021.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            12542..13108
                     /gene="satP"
                     /locus_tag="ACHJ7J_000747"
     CDS             12542..13108
                     /gene="satP"
                     /locus_tag="ACHJ7J_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO4999022.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(13143..13730)
                     /gene="mog"
                     /locus_tag="ACHJ7J_000748"
     CDS             complement(13143..13730)
                     /gene="mog"
                     /locus_tag="ACHJ7J_000748"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO4999023.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(13845..14798)
                     /gene="tal"
                     /locus_tag="ACHJ7J_000749"
     CDS             complement(13845..14798)
                     /gene="tal"
                     /locus_tag="ACHJ7J_000749"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4999024.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            15077..16507
                     /gene="yaaJ"
                     /locus_tag="ACHJ7J_000750"
     CDS             15077..16507
                     /gene="yaaJ"
                     /locus_tag="ACHJ7J_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO4999025.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            16577..17353
                     /gene="yaaA"
                     /locus_tag="ACHJ7J_000751"
     CDS             16577..17353
                     /gene="yaaA"
                     /locus_tag="ACHJ7J_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO4999026.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(17506..17802)
                     /gene="yaaX"
                     /locus_tag="ACHJ7J_000752"
     CDS             complement(17506..17802)
                     /gene="yaaX"
                     /locus_tag="ACHJ7J_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO4999027.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(18016..19302)
                     /gene="thrC"
                     /locus_tag="ACHJ7J_000753"
     CDS             complement(18016..19302)
                     /gene="thrC"
                     /locus_tag="ACHJ7J_000753"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO4999028.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(19303..20235)
                     /gene="thrB"
                     /locus_tag="ACHJ7J_000754"
     CDS             complement(19303..20235)
                     /gene="thrB"
                     /locus_tag="ACHJ7J_000754"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO4999029.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(20237..22699)
                     /gene="thrA"
                     /locus_tag="ACHJ7J_000755"
     CDS             complement(20237..22699)
                     /gene="thrA"
                     /locus_tag="ACHJ7J_000755"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO4999030.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(22725..22844)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(22780..22845)
                     /gene="thrL"
                     /locus_tag="ACHJ7J_000756"
     CDS             complement(22780..22845)
                     /gene="thrL"
                     /locus_tag="ACHJ7J_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO4999031.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(23059..23745)
                     /gene="yjtD"
                     /locus_tag="ACHJ7J_000757"
     CDS             complement(23059..23745)
                     /gene="yjtD"
                     /locus_tag="ACHJ7J_000757"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4999032.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(24145..24285)
                     /gene="yjjY"
                     /locus_tag="ACHJ7J_000758"
     CDS             complement(24145..24285)
                     /gene="yjjY"
                     /locus_tag="ACHJ7J_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO4999033.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            24381..25097
                     /gene="arcA"
                     /locus_tag="ACHJ7J_000759"
     CDS             24381..25097
                     /gene="arcA"
                     /locus_tag="ACHJ7J_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO4999034.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(25157..26509)
                     /gene="creD"
                     /locus_tag="ACHJ7J_000760"
     CDS             complement(25157..26509)
                     /gene="creD"
                     /locus_tag="ACHJ7J_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO4999035.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKDVTMDDQLRIWK
                     "
     gene            complement(26567..27991)
                     /gene="creC"
                     /locus_tag="ACHJ7J_000761"
     CDS             complement(26567..27991)
                     /gene="creC"
                     /locus_tag="ACHJ7J_000761"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO4999036.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDHEHVTLKVLDTGSGIPYYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(27991..28680)
                     /gene="creB"
                     /locus_tag="ACHJ7J_000762"
     CDS             complement(27991..28680)
                     /gene="creB"
                     /locus_tag="ACHJ7J_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO4999037.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(28693..29166)
                     /gene="creA"
                     /locus_tag="ACHJ7J_000763"
     CDS             complement(28693..29166)
                     /gene="creA"
                     /locus_tag="ACHJ7J_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO4999038.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            29377..30246
                     /gene="robA"
                     /locus_tag="ACHJ7J_000764"
     CDS             29377..30246
                     /gene="robA"
                     /locus_tag="ACHJ7J_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO4999039.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(30243..30890)
                     /gene="gpmB"
                     /locus_tag="ACHJ7J_000765"
     CDS             complement(30243..30890)
                     /gene="gpmB"
                     /locus_tag="ACHJ7J_000765"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO4999040.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            30942..31454
                     /gene="yjjX"
                     /locus_tag="ACHJ7J_000766"
     CDS             30942..31454
                     /gene="yjjX"
                     /locus_tag="ACHJ7J_000766"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO4999041.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(31601..31927)
                     /gene="trpR"
                     /locus_tag="ACHJ7J_000767"
     CDS             complement(31601..31927)
                     /gene="trpR"
                     /locus_tag="ACHJ7J_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO4999042.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(32017..33954)
                     /gene="sltY"
                     /locus_tag="ACHJ7J_000768"
     CDS             complement(32017..33954)
                     /gene="sltY"
                     /locus_tag="ACHJ7J_000768"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO4999043.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGATEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTEGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            34165..35832
                     /gene="ettA"
                     /locus_tag="ACHJ7J_000769"
     CDS             34165..35832
                     /gene="ettA"
                     /locus_tag="ACHJ7J_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO4999044.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(35988..36875)
                     /locus_tag="ACHJ7J_000770"
     CDS             complement(35988..36875)
                     /locus_tag="ACHJ7J_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001484212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4999045.1"
                     /translation="MIDLRQLKYFVVVAEEEHVGRAAERLHISQSPLSRQIAQLEERL
                     GLTLFERNQQRIRLTADGQTFLAETRSLLTHANRLESLGKRLGRGEDGGLCIGYIENA
                     MHSGVLSTALRTLRVSRPNVHIALYSQPPATQIEGLRQRSLDFSLVDTPPTDSEQDLE
                     YIQVLDDRMLLALPQNHPLAQEENLVASQLGDEQWIAVIHNDDAHSRDHFVAACVNAG
                     FNPDIRLEAGEPLTALGLVAAGLGLALIQHSLRHNAPEGVVLRELPWMNYSTQLWAAW
                     HKANQRPLVSHFRQILRGE"
     gene            37009..38019
                     /locus_tag="ACHJ7J_000771"
     CDS             37009..38019
                     /locus_tag="ACHJ7J_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015368058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO4999046.1"
                     /translation="MSIKNLLPGKIGLGGAPLGNMFRAIPEEEAHATVSEAWNLGVRY
                     FDTAPLYGSGLSEIRMGEALSQYPRDEYVLSSKVGRIMLDEMEDPAKRDFGEKGGLFE
                     HGLKNKILNDYSADATLRSIEDSLKRLKTDRLDIVWIHDPAQDFYGDSWLEQFNIART
                     GAFRALTRLRDEGVIKAWGLGVNRVEPCELTLALDEPKPDAFLLAGRYSLLDHERALQ
                     RLMPEALEQNVDIVVGGPYSSGVLAGGEHFEYQKASAAIQQKVAQINAIATRFNVNVK
                     AAALQFALANPAVAAVIPGSSRPGRMAEDLAALNAPVPAEFWAEMRRQNLVAENAPLP
                     TR"
     gene            38039..38836
                     /locus_tag="ACHJ7J_000772"
     CDS             38039..38836
                     /locus_tag="ACHJ7J_000772"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO4999047.1"
                     /translation="MKLDLTGKVAVVSGSTSGIGQGIAAGLAKAGATVVVVGRKQSGV
                     DDAMAAIAAQTPTASLRGVVADLSTEDGVAALIAAVPTADVLVNNLGIFNDKDFFSVP
                     DDEWMHFYNVNVLSGVRLARHYAPGMTEQGWGRIIFVSSESGVAIPGDMINYGVTKAA
                     NLAVSHGLAKRLAGTGVTVNAILPGPTFTDGLKEMLADAAAQSGRSAREQADEFVKTS
                     RPSSIIQRAADVEEVANLAVYLASPLSSATTGAALRVDGGVVDTLTM"
     gene            38862..39278
                     /locus_tag="ACHJ7J_000773"
     CDS             38862..39278
                     /locus_tag="ACHJ7J_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HFO4999048.1"
                     /translation="MAKTTVSIEIPASADAVWQLMGGFDALPDWLPFIPKSVVTEGGR
                     VRSLTTSDGGTVVERLEAFDNRQRSYTYSIIQAPFPVVDYLSTITVHETADSQVSRVE
                     WFGEFTPVNVTNEEAEALFTGIYRDGLKALKDNFVA"
     gene            39436..39648
                     /locus_tag="ACHJ7J_000774"
     CDS             39436..39648
                     /locus_tag="ACHJ7J_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFO4999049.1"
                     /translation="MKTIIAIFTASLFSVAAFNASAQSVTATGTTLDSAEAVIAQKAK
                     EMNASEYKITSARMGNTVTMSAELYK"
     gene            39730..39843
                     /locus_tag="ACHJ7J_000775"
                     /pseudo
     CDS             39730..39843
                     /locus_tag="ACHJ7J_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40115..41347)
                     /gene="nadR"
                     /locus_tag="ACHJ7J_000776"
     CDS             complement(40115..41347)
                     /gene="nadR"
                     /locus_tag="ACHJ7J_000776"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO4999050.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEESAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(41368..42750)
                     /gene="radA"
                     /locus_tag="ACHJ7J_000777"
     CDS             complement(41368..42750)
                     /gene="radA"
                     /locus_tag="ACHJ7J_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO4999051.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(42799..43767)
                     /gene="serB"
                     /locus_tag="ACHJ7J_000778"
     CDS             complement(42799..43767)
                     /gene="serB"
                     /locus_tag="ACHJ7J_000778"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO4999052.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            43873..44517
                     /gene="ytjB"
                     /locus_tag="ACHJ7J_000779"
     CDS             43873..44517
                     /gene="ytjB"
                     /locus_tag="ACHJ7J_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO4999053.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            44545..45561
                     /gene="lplA"
                     /locus_tag="ACHJ7J_000780"
     CDS             44545..45561
                     /gene="lplA"
                     /locus_tag="ACHJ7J_000780"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO4999054.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     THAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWIA
                     GAVR"
     gene            45593..45856
                     /locus_tag="ACHJ7J_000781"
     CDS             45593..45856
                     /locus_tag="ACHJ7J_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4999055.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(46017..46736)
                     /gene="deoD"
                     /locus_tag="ACHJ7J_000782"
     CDS             complement(46017..46736)
                     /gene="deoD"
                     /locus_tag="ACHJ7J_000782"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO4999056.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(46816..48039)
                     /gene="deoB"
                     /locus_tag="ACHJ7J_000783"
     CDS             complement(46816..48039)
                     /gene="deoB"
                     /locus_tag="ACHJ7J_000783"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4999057.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(48091..49413)
                     /gene="deoA"
                     /locus_tag="ACHJ7J_000784"
     CDS             complement(48091..49413)
                     /gene="deoA"
                     /locus_tag="ACHJ7J_000784"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO4999058.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(49540..50319)
                     /gene="deoC"
                     /locus_tag="ACHJ7J_000785"
     CDS             complement(49540..50319)
                     /gene="deoC"
                     /locus_tag="ACHJ7J_000785"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO4999059.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGSDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            50577..52127
                     /gene="yjjI"
                     /locus_tag="ACHJ7J_000786"
     CDS             50577..52127
                     /gene="yjjI"
                     /locus_tag="ACHJ7J_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO4999060.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            52099..52962
                     /gene="yjjW"
                     /locus_tag="ACHJ7J_000787"
     CDS             52099..52962
                     /gene="yjjW"
                     /locus_tag="ACHJ7J_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO4999061.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPSQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(53176..53958)
                     /gene="yjjV"
                     /locus_tag="ACHJ7J_000788"
     CDS             complement(53176..53958)
                     /gene="yjjV"
                     /locus_tag="ACHJ7J_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4999062.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(53955..55028)
                     /gene="yjjU"
                     /locus_tag="ACHJ7J_000789"
     CDS             complement(53955..55028)
                     /gene="yjjU"
                     /locus_tag="ACHJ7J_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO4999063.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(55150..55311)
                     /gene="ytjA"
                     /locus_tag="ACHJ7J_000790"
     CDS             complement(55150..55311)
                     /gene="ytjA"
                     /locus_tag="ACHJ7J_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO4999064.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(55438..56043)
                     /gene="osmY"
                     /locus_tag="ACHJ7J_000791"
     CDS             complement(55438..56043)
                     /gene="osmY"
                     /locus_tag="ACHJ7J_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO4999065.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKESSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(56436..58025)
                     /gene="prfC"
                     /locus_tag="ACHJ7J_000792"
     CDS             complement(56436..58025)
                     /gene="prfC"
                     /locus_tag="ACHJ7J_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO4999066.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(58116..58793)
                     /gene="yjjG"
                     /locus_tag="ACHJ7J_000793"
     CDS             complement(58116..58793)
                     /gene="yjjG"
                     /locus_tag="ACHJ7J_000793"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO4999067.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTDESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(58808..59254)
                     /gene="rimI"
                     /locus_tag="ACHJ7J_000794"
     CDS             complement(58808..59254)
                     /gene="rimI"
                     /locus_tag="ACHJ7J_000794"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4999068.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(59223..59636)
                     /gene="holD"
                     /locus_tag="ACHJ7J_000795"
     CDS             complement(59223..59636)
                     /gene="holD"
                     /locus_tag="ACHJ7J_000795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO4999069.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            59739..60770
                     /gene="rsmC"
                     /locus_tag="ACHJ7J_000796"
     CDS             59739..60770
                     /gene="rsmC"
                     /locus_tag="ACHJ7J_000796"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO4999070.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            61038..61124
                     /locus_tag="ACHJ7J_000797"
     tRNA            61038..61124
                     /locus_tag="ACHJ7J_000797"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61072..61074,aa:Leu,seq:cag)
     gene            complement(61163..61399)
                     /gene="yjjZ"
                     /locus_tag="ACHJ7J_000798"
     CDS             complement(61163..61399)
                     /gene="yjjZ"
                     /locus_tag="ACHJ7J_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO4999071.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     MMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            61540..62328
                     /gene="fhuF"
                     /locus_tag="ACHJ7J_000799"
     CDS             61540..62328
                     /gene="fhuF"
                     /locus_tag="ACHJ7J_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO4999072.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQHRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDLQQCGDCTLK"
     gene            complement(62366..63043)
                     /gene="bglJ"
                     /locus_tag="ACHJ7J_000800"
     CDS             complement(62366..63043)
                     /gene="bglJ"
                     /locus_tag="ACHJ7J_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO4999073.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(63001..63726)
                     /gene="yjjQ"
                     /locus_tag="ACHJ7J_000801"
     CDS             complement(63001..63726)
                     /gene="yjjQ"
                     /locus_tag="ACHJ7J_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4999074.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            64347..65117
                     /gene="yjjP"
                     /locus_tag="ACHJ7J_000802"
     CDS             64347..65117
                     /gene="yjjP"
                     /locus_tag="ACHJ7J_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO4999075.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            65108..65581
                     /gene="yjjB"
                     /locus_tag="ACHJ7J_000803"
     CDS             65108..65581
                     /gene="yjjB"
                     /locus_tag="ACHJ7J_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO4999076.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            65688..66227
                     /gene="dnaT"
                     /locus_tag="ACHJ7J_000804"
     CDS             65688..66227
                     /gene="dnaT"
                     /locus_tag="ACHJ7J_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO4999077.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            66230..66967
                     /gene="dnaC"
                     /locus_tag="ACHJ7J_000805"
     CDS             66230..66967
                     /gene="dnaC"
                     /locus_tag="ACHJ7J_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO4999078.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            67016..67510
                     /gene="yjjA"
                     /locus_tag="ACHJ7J_000806"
     CDS             67016..67510
                     /gene="yjjA"
                     /locus_tag="ACHJ7J_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO4999079.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            67764..70055
                     /gene="opgB"
                     /locus_tag="ACHJ7J_000807"
     CDS             67764..70055
                     /gene="opgB"
                     /locus_tag="ACHJ7J_000807"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO4999080.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(70192..71214)
                     /gene="lgoD"
                     /locus_tag="ACHJ7J_000808"
     CDS             complement(70192..71214)
                     /gene="lgoD"
                     /locus_tag="ACHJ7J_000808"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO4999081.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            71353..72267
                     /gene="lgoR"
                     /locus_tag="ACHJ7J_000809"
     CDS             71353..72267
                     /gene="lgoR"
                     /locus_tag="ACHJ7J_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO4999082.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            72482..73843
                     /gene="lgoT"
                     /locus_tag="ACHJ7J_000810"
     CDS             72482..73843
                     /gene="lgoT"
                     /locus_tag="ACHJ7J_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4999083.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(73892..75556)
                     /gene="tsr"
                     /locus_tag="ACHJ7J_000811"
     CDS             complement(73892..75556)
                     /gene="tsr"
                     /locus_tag="ACHJ7J_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO4999084.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWVDYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(75675..76121)
                     /gene="hpaR"
                     /locus_tag="ACHJ7J_000812"
     CDS             complement(75675..76121)
                     /gene="hpaR"
                     /locus_tag="ACHJ7J_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO4999085.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT        18650 a        20108 c        18732 g        18645 t
ORIGIN      
        1 ataccagtta attaaggatg atttatgaaa aagtatatta ttcccttcgt tgcgacaacg
       61 ttgatgttta gcgcaacaac cttcgccaag gatgtcgatc aaggaacact aaccattaga
      121 ggagaagttg ttgatacaac gtgttacttt gttaatggtg attcgacaac agatatcgtt
      181 gtcgaagatg ttgtaaccag tgcaatgaat caacttatca ataatgaaat ctatacttta
      241 aaaaccggtt caacctctca tgacttagaa attcagtgtg acggaaatac agccccaagt
      301 atcgaagtac tagcatcaga gtttgatgct aataacttta cgctgaataa aggaactgct
      361 gagaacattg gctttgccat tttcctcgat gatgggactt tgaccagaat gagaccaggg
      421 ttaaaaacac cgctgaaaga taatggctct ggtcaatatt ttcttaattt gaatgcacaa
      481 tatgcccgca caagtggcaa tccagttaca aaaggctccg tagagtcaac cgtaacgatt
      541 aaaatcagtg cagattaacg ttttttgccg gtagctctgc taccggtatt atttacggag
      601 tgattacgtg agaataataa cagcattact gatgtcattt tttattttac ctgttcatgc
      661 cggtgtggtc atctatggta cgcgcattat ttatcctgaa aaaaacagag aagttctcgt
      721 acagttaatg aatcaaagta aaaatgcctc tttaattcag gcatggatcg atgatgggaa
      781 caccactatc gcaccagaaa aaattcaggt cccatttatt ctcacaccgc cagtaagtcg
      841 tgtagcaggt ggttccggcc aacaacttaa aataagaaaa atgccaaaca atttgcctca
      901 caataaagaa agtttatttt atctaaatgt tctggatatc cctccaaaca atccacaaaa
      961 tgcaggaaaa aacaaaatta aacttgcatt acaaaaccgg ataaagttac tttggcgtcc
     1021 aagcggaata gctccggtag ataaaaaaag cctttctcaa ttaaatatca aaaagaaaaa
     1081 taacgccatc tcaataaaca acgaaacagc caactggata acggttacaa ctatcaaagc
     1141 acaaaacgtg aaagttaata atgaaagtat cttactgcct ccattttcga ataacgacat
     1201 tacattaaaa aataaccacg catcagaata tgaactaacg gtagttgatg attacgggaa
     1261 taacatccat agtaaaatcg ccgcgagata acaggaaaaa gtcatgttaa ggacgacacg
     1321 atgggtcgct gctattattt ttttgtattc ttttccgggt tatgctgaag aaacttttga
     1381 tacgcacttt atgattggcg gaatgagagg agaaaaagtt tccgaatatc gtttcgataa
     1441 taaacaaccg cttccaggaa actacgaact tgatttttat gtaaataacc agtggcgtgg
     1501 aaaacaagat atcacgatcc cagagtcacc agtcaaacca tgtctgccta aggtgctact
     1561 aaccaaactg ggggtaaaga ccggtaacct caatacagaa gataattgta ttttactgga
     1621 tgaagcggtt catggtggac aatatcagtg ggatatcagt gaacaccgac taaatttaac
     1681 agtaccacag gcgtatatca atgaacttga aagaggctac gttcccccag aaagttggga
     1741 tcgcggcatt gatgcttttt atacctccta taatttaagt cagtatcgct cttatgacag
     1801 caataataac agtcatacag ccagttatgg ccggtttaac agcggattaa atttatttag
     1861 ttggcagtta cactctgatg ccagttatag caaacctgat gatatgaaag gcaaatggca
     1921 aagtaatact ctttacctgg aacgtggctg gtcgcagata ttaagcacag ttcagatagg
     1981 agaaaattac acatcctcac tcatattcga ctctctgcga tttagcggaa tacgcttatt
     2041 tcgcgatatg caaatgctac ctgattctat gcagagcttt acacctttag tacaaggtgt
     2101 tgcgcaaagt aacgccttga taacggtttc gcaaaatggc tataccattt accaaaaaga
     2161 ggttccccca ggaccattta ctatcgcaga tttgcaactt tcgggcagcg gctctgatct
     2221 cgatgtcagc attaaggaag cagatggtag tgttcgttcc tttttggtgc cctactcttc
     2281 cgtgcccaat atgctgcaac ctggtgtttc aaatttcgat tttatcgccg gacgtagtca
     2341 gatatatggt gtcaagaatc aggaggactt tctcgaggca aattatatat atggcctcaa
     2401 caatcttttg acactttatg gcggtacgat tttatccgat aactataatg ctataactct
     2461 gggaaatggt tggaatacac cactgggtgc catctctttt gacgctaccc gatcgagtag
     2521 taagttaaat aatgatacca gacacgaagg tacgagctat caggtcgcct ataataaata
     2581 tctattgcaa acggccactc actttagcgt ggccgcctgg cgttatgctt cgcaggatta
     2641 caggacattc agcgaccatc tttacgaaaa tgataaaata aatcatcaga gtgactatga
     2701 tgatttttat gatattggca gaaaaaatag cctttctgcc aatataatgc aacctttatc
     2761 caataatctg ggaaatgtat cattaagtgc actttggcgg aattactggg ggcgaagtgg
     2821 aaatgctaaa gattaccaat tcagttactc caataactgg caacacatta gttatacttt
     2881 ctctgccagc caatcctatg atgaaaacga taaagaagag gagcgtttta atctgtttat
     2941 ctccattcct ttctactggg gtgatgatat tgccaaaaca cgtcaccaaa ttaacttatc
     3001 gaattctacc tcattttcca aagatggcta ttcctccaac aatactggaa ttactggcat
     3061 agccggtgaa catgatcagt taaattatgg tatatatgtt aatcagcaac aacaaaataa
     3121 tgatacctcg cttggtacga atttaagctg gagaactcca atcgccacaa tagatggcag
     3181 ctatagccat tctaaaaacg cctggcaaag tggtggaagt attagtagtg gattagttgt
     3241 ctggcccggt ggtattaata tcactaacca gctatccgat acatttgcaa ttctggatgc
     3301 gcctgggtta gaaggcgcgc atattaatgg acaaaaatac aaccgaacaa acagcaaagg
     3361 ccaggttgtt tacgacctga tgatacctca tcgtgaaaac catctggtac ttgatacagc
     3421 aaacagtgaa agtgaaacag aattgcaggg caatcgtcaa attattgcgc cttaccgtgg
     3481 agcagtttct tatgtgcagt ttacaactga ccaacgtaag ccctggtata tacaggcact
     3541 gcgtcccgat ggttcgccat taacttttgg ctatgacgta ctggatctcc aggaaaacaa
     3601 tattggagtc gttggccagg gtagtctcct ttttattcgc gtagatgaaa ttccaactgg
     3661 cataaaagtt gctctcaatg atgaacagaa tttattctgt actattactt ttcaacacgt
     3721 tatcgatgaa aacaaaacat atatatgcca gtaaaaggag tttactatga agtggctttt
     3781 attaatcacc ttgagtctat attcttttat tgtgcaaagt gctccctgtg gattgaccaa
     3841 tatcggggaa caacgtggaa catatatact caagccactg agtatgaaag gtaatctcac
     3901 cgccgaaaaa attgaaaaca ccggaaatat cattgagtgt gcagacttgc aagagacgac
     3961 taacgccatt aaagttcatt ttttaacaca gaacgcttta aggtttaaag tcggaagtcg
     4021 tcattatatt attaatttcc cctttgaatc tgacggtaaa aaaatagagc ttccagggaa
     4081 gtcatcttat aatttagagg agatgctatc tgcgataaat ttcaccattc catactctat
     4141 cgcatctgta tcagatagtt ccaacaccgc agatattttt ccaggaactc cttttccttt
     4201 gttgttcgaa attgaagtga gttcatgtac agggaataat actaattgta atagcgtccg
     4261 ctttaatttt aatttaaata ctattttaca ggtaaattta atgacctgtg gttttgaaga
     4321 tcaatccatc gacactggac gatttgtact gtctcgtatc aataatgaaa attatcagtt
     4381 tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt ttgaacccag
     4441 tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca gcatattaaa
     4501 aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac tctccaatga
     4561 tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc atccccatga
     4621 aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca atgtctcaag
     4681 cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta tctcccccac
     4741 cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc agaataatcc
     4801 gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa aatagcatgg
     4861 tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc ggcataaaag
     4921 tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc aaaagctttc
     4981 atcaaagcgg catcatcaaa ctcgccaagg atttctacgt ttaatccctg ggagttaaac
     5041 cagttaagta atttgcgccc taacattgaa cgtcgcccag gaatcaaaag ccgccgttct
     5101 tcaagacaag ctgggaacgg tttttccggc ggtggatttg tacaccagaa actcacgcca
     5161 cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagaatctat cggacagtcg
     5221 gaaatgatca tatccagttt atgctggctt aattgctcca gcagcatttc gtgggtggat
     5281 tcgaagcagc gaagatgaat cggctcgcct tctactactg cagcattcag cacgctactg
     5341 accagacgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt ggattctttg
     5401 cgatagttca caatatccag catttcctgg cttaaggtga acattttatc ggcatagcga
     5461 tagaccagtt ctcccagctc gctgggctcg agaccacgtc ccttgcgctt aaataatttg
     5521 ccttgcaggc gctcttccag cgcccgaatc tgtccggtaa tggtttgtgg tgttaaataa
     5581 agcgcctccg ctgcgccaac cacagaacct tctttataaa catgccagaa gtaatacaag
     5641 tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga aaagggagcc
     5701 gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga acgcgtaacc
     5761 agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt ttcgcccagt
     5821 taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata gacatagtaa
     5881 aaccgatacc gcacaggatc cccaccgcca taatttgctg ataagtcgtt ccctcaggca
     5941 gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc agcggtttgc
     6001 caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca tccagcgtga
     6061 cgccttgcag tgaaacgcca gcattagcaa atgcaaacag tggtaaaatc agatacgcca
     6121 cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc ttctctttca
     6181 aaggaataaa gaagccgaca attacccccg ccagggttgc gtgaaccccc gatttcaaca
     6241 ctgcagtcca caacactacg ccaaccagaa tatagacgcc cgtgcggcgt acaccacaca
     6301 gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagagag gccatcgata
     6361 agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg ataatagcca
     6421 gagccatcaa aaagatcttc agcgctaacg gaacacgact tcccaatagc gccagcacac
     6481 caagtgcaaa ggcaatgtca gtagccgccg ggatcgccca cccttcgcgg gtaatcggat
     6541 cggcatagtt aaaggccaga tagagtaatg ccggcacaat catcccacca atagcggcga
     6601 taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt tcacgtttaa
     6661 cttccagacc gactaacagg aaaaataccg ccatcagcgc gtcatttatc cataacagca
     6721 tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc agaaagtcgt
     6781 gataccatcc actggttgcg ccgctgttgg ccatcatcat cgccaggata gcggcaatga
     6841 taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt ttcacttttt
     6901 atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa tcatcgttaa
     6961 aatcagatta tagtgtagga ataattcgtt ttttacgatc tatagaagaa tttgcgccat
     7021 cagcggtgag atgcgttttt gacggctgta agcgctgacg ggtgacccta agccctgata
     7081 atcatagctt aaggtgagga tttctgaggg attaattagg aaaagtaata attaatttgt
     7141 tgtcagtcgg cgatgtttgt taggccaata tttcaaaaat aatccagctt gtcgtcaata
     7201 attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat tacgccataa
     7261 aaatgcgagg cgtcttccgg attagaatcg ataaaacaga atgggttaaa gagagcgata
     7321 taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg cagatagaga
     7381 aaagccccga gtgatatttt accatcaacc cgaggccccc tatatgctga acacatgtag
     7441 agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc ataaggcgat
     7501 gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg taacgagaaa
     7561 agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt tcacggctta
     7621 cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg tgctcgcata
     7681 tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg gggaggttag
     7741 cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga gcgcgggctg
     7801 ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag ctgtttctgc
     7861 ttctcgttca ggcctacagg tgtttcgacg acaacgcggc acagcaaatc accctgtgcg
     7921 ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc ggtctgggtt
     7981 tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat ttcgccaccc
     8041 agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc ttcacgctcg
     8101 aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg tgcgccatgc
     8161 tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac ccctgccggg
     8221 attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca tttgttgcac
     8281 ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg cacggcaaag
     8341 aaaccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt ctgcggctgt
     8401 gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt cggaatgcgg
     8461 atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt atagcgtaaa
     8521 tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc gccgaaaacg
     8581 tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac cgccgccgcc catgccacct
     8641 tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga gtcggtcaga
     8701 acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc accctggtta
     8761 cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttttgatttc acgctcttcc
     8821 gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt ttttaaattg
     8881 cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca gtataattac
     8941 ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt cgacaacatc
     9001 gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct gggcatgttg
     9061 ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca ttttcgcttc
     9121 gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg cagactcgat
     9181 agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct tacgggtgct
     9241 gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact tacggtcagc
     9301 ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt tcagaccaga
     9361 agacgccttg atggtgatct tctgctcttt accgctgttt ttatctttcg cggaaacgtg
     9421 caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc cgcgcggtgc
     9481 cgggttgata ccatccaggt tgaactgacc cagagatttg ttatcagccg cacgtttacg
     9541 ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcag tagagaacac
     9601 ctggctgtgc ttggtcggga tagtggtgtt tttcgcgata agcgtggtca tcacaccgcc
     9661 catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt cttttacgtc
     9721 accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt ccgggttaac
     9781 gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca ttggcatacg
     9841 agtctgacca ccaacgagga taacgtcgtc gatatcagat acagacaggc cagcgtcctg
     9901 cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca ggctttccag
     9961 tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg cagtgatgta
    10021 cggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt tttctgccgc
    10081 ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct gatctttctt
    10141 gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac ccaggtgggt
    10201 atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt cgatttcaat
    10261 aatagaaata tcgaaagtac caccacccag gtcataaacc gcgatagtac ggttgccagt
    10321 acctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga tacgttttac
    10381 ttcgagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat cgttaaagta
    10441 tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt cagcggtttt
    10501 cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct ggcctttaac
    10561 ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga tggaaacatc
    10621 acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg caaacagggt
    10681 gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag tttcaccatc
    10741 ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcattct ccagcacacg
    10801 aggagtggtg ccatccataa tcgctacaca agagttggta gtacccaagt cgataccaat
    10861 tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt gtgagtctgt
    10921 aataggggcg aaacgtctgg tttcaactgc ccaatttcat tttttttgca gactcactca
    10981 ctgcggttga ctactaaatg gggtcgtaac ccacgtcatc aagggggaga tgcaaaaatt
    11041 ttttgtgctt ttgcgcagaa atttcgtgat atggcaggac aagacgtaaa attgacagca
    11101 attttattca tataaagaat gaatcgcagt caaggtattc atcaaaaaat tgtaataaaa
    11161 agaaaagatt acgtgcctga atcttctctt tatcagcagt aaactagtgg gtattcatcc
    11221 ccctacctct tcccactaag agaatcctta tgaaatccgt ttttacgatt tccgccagcc
    11281 tggcgattag cctgatgctg tgctgcacgg cgcaggcaaa cgaccataaa ctcctcggcg
    11341 tcattgcaat gccgcgtaac gaaaccaacg atctggcgct gaaacttcct gtttgtcgca
    11401 ttgtgaaacg catacaactc tctgccgacc atggcgattt acagttaagc ggtgcatcgg
    11461 tttatttcaa agccgcccgt agcgccagtc agagcctgaa tattccttca gaaataaaag
    11521 aagggcaaac cactgactgg atcaacatta acagcgataa cgacaataaa cgctgcgtct
    11581 caaaaatcac cttttccggt catacggtga actcatcgga catggccacg ctgaaaatta
    11641 tcggcgacga ctaacgccag aatatagaag ccacaaaaaa tgaatgttaa ttacctgaat
    11701 gattcagatc tggattttct ccagcattgt agtgaggaac agttagcaaa tttcgcccgg
    11761 ctgctcaccc ataatgaaaa aggcaaaact cgcctctcca gcgtactgat gcgcaacgaa
    11821 ctgtttaaat cgatggaagg ccatcccgag caacatcgcc gcaactggca gctgattgcc
    11881 ggagaattac agcattttgg tggcgatagt atcgccaaca aactgcgcgg acacggtaaa
    11941 ttgtatcggg ccattttgct cgatgtttca aagcgattga agctgaaagc cgacaaagag
    12001 atgtctacgt ttgaaattga gcaacagtta ctggaacaat ttctgcgtaa tacctggaag
    12061 aaaatggacg aggaacataa gcaggagttt ctgcacgcgg tcgatgccag ggtgaatgag
    12121 ctggaagagc tgctgccgct gctgatgaaa gacaaattat tggcaaaagg tgtgtcgcat
    12181 ttgctttcca gccaattgac ccgcatttta cgcacccacg cagcaatgag cgtgcttggg
    12241 catggtttgc tgcgcggcgc gggtctggga ggccctgtgg gcgcagcatt aaatggggtt
    12301 aaagcggtca gcggcagcgc ctatcgcgtg acgattccag ccgtactgca aatcgcctgc
    12361 ctgcgccgga tggttagcgc cactcaggtc tgataccgga aaccggggaa aattttcatt
    12421 ttcccctcaa aagatcgtag acactgcccc actggctgat tattatgccg cgccctgaaa
    12481 acactacagt tattcaggga aattatttca ccattcattc gatgatgatt tttgaggaat
    12541 tatgggcaac actaagttgg ctaatccggc accgctgggc ctgatgggct tcggcatgac
    12601 caccattctg cttaacctgc acaacgtggg ttatttcgct ctggacggta ttattcttgc
    12661 catgggcatt ttctacggcg gcatcgcgca aatttttgcc ggtctgctgg agtacaaaaa
    12721 aggcaacact ttcggtttaa ccgcattcac ctcttacggt tctttctggc tgacgctggt
    12781 tgcgattctg ctgatgccga aactgggtct gaccgatgcg ccaaatgcac agttccttgg
    12841 tgtctacctg ggtctgtggg gcgtatttac gctgtttatg ttcttcggca ctctgaaagg
    12901 cgcacgcgtc ctgcaattcg ttttctttag cctgaccgtg ctgtttgccc tgctggcgat
    12961 cggtaacatt gccggtaatg ccgcgatcat ccactttgcc ggctggattg gtctgatctg
    13021 cggtgccagc gcaatctatc tggcgatggg tgaagtgctg aacgagcagt ttggtcgcac
    13081 cgttctgccg attggtgaat cccactaatt gtcttgagat ccccccgctc ggggggattt
    13141 ttttattcgc taacatcgcg tcttgcactc ttcggtctga atgcagcaac cacttctggt
    13201 gccgtttcaa cgtatggccc ttccagcaac tgaatgcagt acggtacgct ggcaaaaata
    13261 ccgtgtacca caacgttacc ctcagcgtcc ttcacacctt ccagcgtctc tttaatagac
    13321 ttcggctgac cgggtaagtt aaggatcagc gcctgtttgc gaatcacacc cacctgacgc
    13381 gaaaggatcg cagttggtac aaaatgcagg ctgatctggc gcatctgttc accaaagcca
    13441 ggcatctcgc ggtccgctac tgccagcgtc gcatcgggcg ttacgtcacg acgcgccggg
    13501 ccagttccgc ccgtggtgag caccagatgg caactcattt catccaccag ctcacacaac
    13561 gtttgctcga tgatcgcctg ctcatcgggg attaagcggg tttccagttc aaacggcgtg
    13621 gttagcgccg atgtcagcca ttcttccagt gcagggatgc ctttatcctg ataaacgccg
    13681 ctggatgcgc gatcagagat ggaaactaag ccaatacgta aagtattcat caagattttt
    13741 ccgtttaaac agtctcgtta aacagaatga tacactgcga agggagtgac agacaggctt
    13801 cagaagaggt agcgtgaccg gcttcccggt cacgctaaga atgattacag cagatcgccg
    13861 atcatttttt ccagtttttc ctggtcaaca gcaaacttac ggataccttc cgccagttta
    13921 tctactgcca ttggatcctg gttgtgctgc cacaggaact cggactcagt gatacgcgcc
    13981 ggacgcgctt tcacttcgcc ggtgtaagac agtttacgtt cgatagcccc ttcgctctcc
    14041 gccagctctt tcagcagtgc cggtgcgatg gtcagacggt cgcagcctgc cagttccaga
    14101 atttcgccga tgttacggaa gcttgcgccc ataaccacgg tttcataacc gtgctctttg
    14161 tagtactggt agatttcaga tacagaaacc acgcccggat cttctgccgg agcgtactct
    14221 ttcttatcgg tattcgcttt gtaccagtca agaatacggc caacaaacgg cgagatcagg
    14281 aacacgcccg cttccgcaca agcacgagcc tgagcgaagg agaacagcag ggtcaggtta
    14341 cagttgatgc cttctttttc cagctgttct gcagcacgga taccctgcca ggtagaagcc
    14401 agtttgatca gaatacgatc gttgctaata cctgcatcgt tgtagagttt gatcaggcgt
    14461 tttgctttcg caattgacgc ttcggtgtca taggaaagac gcgcatcaac ttcagttgag
    14521 atacggcccg gaaccagttt caggatttcc agaccaatat ttactgccag tttgtcggtc
    14581 gcgtccacga tctgctgcgc gcgatcgttg ctctgctgtt tcgcccaggc gacagcatca
    14641 tcaatcaact tacgatattc cggaatctgc gctgcgttaa gaatgagaga agggttggtt
    14701 gtggcatcct gcggttgata cagcttcatt gccgcgatgt ccccagtgtc ggccactacg
    14761 gtggtgtact gacgaaggga ggtcaatttg tccgtcatga tagtatttct ctttaaacag
    14821 cttgttaggg ggatgtaacc ggtctgccct gatgatatca cgacgcaacg tcagcgcaac
    14881 cgccgacaag acggttatcg ccaggcgaga ctgtttcgga tttctgacac tgcgttgata
    14941 tcgcccgcca tttttaaaca aaacctcatg tatgctacgc agaagttatc aagtacctcg
    15001 tagcgtatat acttcttaaa caattggtaa cgtttacaca ggaaagtcat cgcgaccggc
    15061 aataagaggg atatgcatgc cagatttttt ctcctttatt aacagcgtcc tttggggatc
    15121 ggtaatgatt tacctgctct tcggcgcagg ttgttggttc acttttcgca ccggatttgt
    15181 gcagtttcgc tacatccgcc agtttggcaa aagtcttaaa aatagcattc atccacagcc
    15241 aggcggttta acctcatttc agtcattgtg taccagtctt gcggcgcgcg tgggtagcgg
    15301 caacctggcc ggcgttgcgc tggctattac cgccggtgga cctggagccg tcttttggat
    15361 gtgggttgcc gcgtttatcg gcatggcgac ctcgtttgcc gaatgttccc ttgcacaact
    15421 ttataaagaa cgtgacgtca atgggcagtt tcgtggcgga ccggcatggt atatggcgcg
    15481 cgggctgggg atgcgctgga tgggcgttct gttcgccgtc tttttgctca tcgcctacgg
    15541 cataattttc agcggagttc aggcaaacgc tgttgcccga gctctgagtt tttcttttga
    15601 ttttcccccg ctggtgacag gcattattct cgctgtcttt gctctgctgg caatcactcg
    15661 cggtcttcat ggcgtcgccc ggctcatgca ggggtttgtc ccgttgatgg cgataatctg
    15721 ggtactgacc agcctggtga tttgcgtaat aaatatcggg caacttcccc acgtcatttg
    15781 gtctattttt gagagtgctt ttggctggca ggaagcggca ggcggcgcgg cgggatatac
    15841 cttaagccag gcgattacta acggttttca gcgcagtatg ttttccaatg aggcgggaat
    15901 gggttcgacg ccaaacgcgg cagcggcagc ggcgtcctgg cctccgcatc cggcagcgca
    15961 aggaattgtc cagatgattg gcatttttat cgacaccctg gtcatctgta cggcaagcgc
    16021 catgctgata ttactggcgg gtaacggcac aacctacatg ccgctggaag gtattcagct
    16081 tatccagaag gcgatgcggg tgctaatggg ttcctggggt gctgagtttg ttaccctcgt
    16141 ggttattctg tttgccttca gctccatcgt tgccaactac atttatgccg aaaacaatct
    16201 cttcttttta cgcctgaaca accctaaagc gatctggtgt ttgcggatct gcaccttcgc
    16261 aacggtcatc ggcggcacct tgctaagtct tccgctgatg tggcaactgg cagatatcat
    16321 aatggcctgc atggctatta ccaatttgac cgccatttta ctgctctcgc ctgtggttca
    16381 taccattgcc agtgattatc tacgccagcg taaactcggc gtgcgcccgg tgtttgatcc
    16441 gttgcgttat ccggatatcg gtcgccagct ttctcgggac gcgtgggatg atgtttcgca
    16501 ggagtaatca caactatcga tcaactcatt ctcgtttttt gctaaagtcg gcataaattt
    16561 cctgcaagga ctggatatgc tgattcttat ttcacctgcg aaaacgcttg attaccaaag
    16621 cccgttgacc accacgcgat atacactgcc ggagctgtta gacaattccc agcagttgat
    16681 ccatgaagcg cggaaactga cgcctccgca gattagcacg ctgatgcgta tcagcgacaa
    16741 actggcaggt atcaacgccg ctcgctttca tgactggcag ccagatttca cgccggagaa
    16801 tgcccgccag gcgattctgg cgtttaaagg tgatgtctac accggcttgc aggccgaaac
    16861 cttcagcgaa gacgatttcg attttgccca acagcatttg cgaatgcttt ccggcttgta
    16921 tggcgtactc cgcccgctcg atttaatgca gccttatcgt ctggaaatgg ggatccgtct
    16981 tgagaatgcc cgagggaaag atctgtatca attctgggga gatatcatca ccaacaagct
    17041 gaacgaggcg ctcgcagcac aaggcgataa tgtggtgatt aatctggcgt cagatgagta
    17101 ttttaaatcg gtgaagccga agaaattgaa tgccgagatt atcaagccgg tgttcctcga
    17161 tgagaagaac ggcaaattta agataatcag cttctacgct aagaaagcac gcggtctgat
    17221 gagtcgtttc attattgaaa atcggctgac caaaccagag caactgactg gttttaatag
    17281 cgaaggttac ttctttgatg aagcttcctc cagcaatggc gaactggtgt ttaaacgcta
    17341 cgagcagcgt taagtttgca gtcaatgccg gatgcggcgt gaacgcctta tccggcctac
    17401 aaaagcatgc agattcaata tattgcagaa ttcacgtagg cctgataaga cgcgccagcg
    17461 tcgcatcagg cgctgaatgc cggattgtta cccggcattt gtcatttagc ggtgatgttt
    17521 gcctggacca tgaccgccgt gatgatcatg atgagctttc ttatggtgac gcggcggtgg
    17581 cggcggtccg tgtgggtgcc agcgattgcc tcgccattca taatgttgtt tccaccagcc
    17641 gtggtcgcgc cagtgaccgc catcccagta ataaccgcga ttatcacgat cgcctatctg
    17701 taattttact gacgggacta acgtaatttc cgcagcctgc gttgccatgg gagcgaccag
    17761 aaccagggaa agtgcgagta cgatagattg catctttttc acaggttact ccttttatca
    17821 ttgcccgtta tgggtcgata tccggagatt acgcgatgag aacggcggtt gttattctcc
    17881 gcaatcctaa tctctaagtt accgcattta ttgagaattt ctcctttttc cctgtcgttt
    17941 ctctccatat tttcttcaca aaaaagccgg gctgcataaa agcaaacccg gcctgattga
    18001 gataatgaat agatgttact gatgattcat catcaattta cgcaacgcag caaaatcggc
    18061 gggcagatta tgcgaaagca agggtaaatc ggcacgttct gccagctctt ttggcagatc
    18121 caacgtttca ccgagaatcg cttccacgct ctctttaaat ttcgccggat gcgcggtgcc
    18181 gaggaacaag ccatattcgc ctggattcaa ctggtcacgc agcgcacgat acgctacggc
    18241 agcgtgcggc tccgaagtgt agcccagttc ttttaactca cgcattgtct gttgcgtggt
    18301 ttcatcatcc actgctgcat aacccagctc tttcagttgc cagattttgc ggcggaacaa
    18361 ctcttccaca cgcggccagt tgttcggctg actcacgtcc atcgcgttgg ataacgtcgc
    18421 ctgagtcgct ttgggtgacc actgaccgtc atgcaggaaa cgtggcacgg tatcgttcac
    18481 gttggtcgca gcaataaaac gtttcaccgg cagaccgagt gacttcgcca gcagacccgc
    18541 cgtcaaatcg ccgaagtttc cgcttggcac cgagacaacc agctggttgc gcgcttcctg
    18601 cggcagctgc gcaactgctt caaagtagta gcaaatctgc gccagcaacc ggctgatgtt
    18661 aatcgagtta gctgagttta accccagcgc cactttcagc tcttcatcat caaacgcctg
    18721 cttcaccagc gcctgacagg catcgaaatc gccgtcgatg gcaacagttt cgatattgcc
    18781 gcccaatgta cagaacagtt tttcttgcag tggactgatt ttgcctcgtg gatagaggat
    18841 aaccactttc acattcggta aaccgtagaa agcatgagcc actgccgctc cggtatcacc
    18901 agaggtcgcg gtcagaatgg tcactggctt atcgcccgca atatgggtca gcatttgtgc
    18961 cataaagcga ccgccgaaat ctttaaatgc cagcgttggc ccgtggaaca attccagaca
    19021 accgacatcg ctttcaacat tggcgaccgg agccgggaag gcaaacgccg cgcgcacgcg
    19081 ctcttccagg atttcctgcg ggatttcatc accaataaac gccgagagga tcttcgcact
    19141 gcgggtgaca aaatccagct tcagcatctc atcaatttca gtcaggctga attccggcag
    19201 gtcgtgcgga aaaaacagcc cctgattttt gcccaacccc tgggttacgg cttgcgcaaa
    19261 gctgacctgc tcattgtgat ctttcagatt gtagagtttc atttagtttt ccagtactcg
    19321 tgcgcccgcc gtatccagcc ggcaaatatg aacaaaacct tcctgatttt gcaggtagtt
    19381 cttacccaac cagtcggcaa cgcgctgggc ggtatccggc ttgtcacaca gagcgaacaa
    19441 ggtcgggccg gagccggaga taccgctcgc taccgcgccg atttccgcaa ccgcctgccg
    19501 cgcctgccgg aagccaggca gtaaccgttc acggtagggt tctgcgataa catctttcat
    19561 cagcttcgcg gcaagctcag gctgacggga atagcaggcg tgaatgaagc ctgccaagtg
    19621 tcgcccgtgc gcaatgcaat cctggcggcg atactgcgcc ggtaaaatag ccctggcttc
    19681 tgccgtcgag actttaatcc ccggatacgc cagcacccac agccactcat caaaccctgg
    19741 cacttgctgg ctgatgatgt cgttttcttc gatcatcaac tgcataccac caagaaaaca
    19801 cggtgccacg ttgtcgtaat gaatgctgcc ggagatacgc ccttccagct cgcccatcaa
    19861 agccagcaaa cgagtgtcat taagcggctt gccgcagtgt tcattcatcg ccatcagcgc
    19921 cgcgaccacc gaacaggcgc tggagcctaa gcccgaaccg atcggcatat tcttttccag
    19981 tgtcatcgcc actggaattt gcttgcccag ctcctggcaa aaacgctccc agcactgata
    20041 aacgatattc tcccgtggtt ctgacggcag cttatcggca aaacgtccga ggttgttgag
    20101 actgaatgtc tctgccgcct caaccgtgac tacatctccg agcaatgcac catcaacggg
    20161 tgtcaccgcc gccccgagca catcaaaccc gacgctcata ttggcactgg aagccggggc
    20221 ataaacttta accatgtcag actcctaact tccatgagag ggtacgtagc agatcggcaa
    20281 agacaccggc agcggtaacg tcattgcccg caccatatcc gcgcagcacc aacggcagcg
    20341 gctgataata gtggctatag aaggccaggg cgttttcgcc atttttcact ttgaacagcg
    20401 gatcattacc atccacttcg gcaatcttca cgcggcagac gccatcttca tcaatattgc
    20461 caacatagcg caaaactttt ccttcatcac gggccttcgc cacgcgcgcg gcaaagagat
    20521 cgtcgagctg tgacagattc gccataaaag cggcaacatc accctcggcg ttaaactctg
    20581 cgggcagcac tggttcaatt tcaatatccg ccaactccag ttcacgtccc gtttcacgag
    20641 cgagaatcaa tagtttacgc gctacatcca taccagaaag atcatctcgc ggatccggtt
    20701 cggtataacc catttcccgc gccagcgtag tcgcctcgga gaaactcatg ccttcgtcta
    20761 acttgccgaa gatataagaa agcgaacctg aaagaatgcc ggagaacttc atcaattcat
    20821 caccagcatt gagcagattt tgcaggttct caataaccgg taatccagcc ccaacgttgg
    20881 tgtcatagag gaatttacgc cgcgattttt ccgccgcatg acgcaacaga tggtagtaat
    20941 ccatcgacga ggtgttggcc tttttgttcg gcgtgacaac gtggaaacct tcgcgcagga
    21001 agtcggcata ttgatccgcc actgcctggc tggaggtgca gtcaacaatg accgggttca
    21061 gcagatgata ttctttcacg aggcgaatta agcgcccgag attaaacggc tctttggctt
    21121 gcgccagttc ttcctgccag ttttccaggt ttaggccatg cacattggtg agcagagcct
    21181 tcgagttggc aacgccgcag acacgtaagt cgatatgttt attcttcaac cagctttgct
    21241 gacgcttcag ttgctccagc agcgcaccgc caacgccacc gacgccaatc acaaacactt
    21301 cgataacctg atcggtattg aacagcatct gatgagtaac gcgcacgcca gtggtcgcat
    21361 catcgttatt taccacgaca gagattgagc gttcagaaga tccctgagca atggcgacaa
    21421 tgttgatatt ggcgcgggcc agcgcggcaa agaatttcgc cgagatccca cgcaaggtgc
    21481 gcataccatc acctaccacc gagataatgg ccagccgttc cgtcaccgcc agcggctcca
    21541 gtaagccttc tttcagttcc aggtagaact cttcctgcat tgcccgttca gctcgcacac
    21601 agtcgctttg cggaacgcag aaactgatac tgtattcgga agatgattgc gtaatcagca
    21661 ccacggaaat acgggcgcgt gacattgcag caaagacgcg cgccgccatg ccgaccattc
    21721 ctttcatccc cgggccggaa acgctgaaca ttgccatgtt attcagattg gaaatgccct
    21781 tgaccggtaa ttcgtcttca tcacggctgg caccaatgag cgtacctgga gcttgaggat
    21841 ttccggtatt tttaatcagg cacgggatct ggaactgggc gatgggggta atggtgcggg
    21901 ggtgaagaac tttagcgccg aagtaggaaa gctccatcgc ttcctggtag gacatcgact
    21961 tcaacaacct cgcatcgggc acctgacgcg ggtcgcaggt ataaaccccg tcaacgtccg
    22021 tccaaatctc gcaacaatcg gcgcgtaaac aggcagccag caccgccgcg gagtagtcgg
    22081 aaccgttgcg tccaagtacc accagttcgc ctttttcatt accggcggtg aaacctgcca
    22141 tcagcaccat gtgatcagcc ggaatacgac ttgccgcaat acggcgggtg gactcagcaa
    22201 tatcgacggt agattcgagg taatgcccca ctgccagtag tttttcgacc ggatcgataa
    22261 cggtaacgtt gtgaccacgc gcttctaaca cgccggccat aatggcgatt gacattttct
    22321 cgccacggca aatcagcgca gcgttaatgc tatccgggca ctgccctaac aaactaatgc
    22381 catgcaggac atgttttatt tgggcaaatt cctggtcgac gaaagttttc aattgcgcca
    22441 gcgggaatcc cggctgggcg gcggcgagtc ccgtcagaag ttcggcaaaa atacgttcgg
    22501 catcgctgat attgggtaaa gcatcctggc cgctaatggt tttttcaatc atcgccacca
    22561 ggtggttggt tattttggcg ggggcagaga ggacggtggc cacctgcccc tgcctggcat
    22621 tgctttccag aatatcggca acccgcagaa aacgttctgc atttgccact gatgtaccgc
    22681 cgaacttcaa cactcgcatg gttgttacct cgttaccttt ggtcgaaaaa aaagcccgca
    22741 ctgtcaggtg cgggcttttt tctgtgtttc ctgtacgcgt cagcccgcac cgttacctgt
    22801 ggtaatggtg atggtggtgg taatggtggt gctaatgcgt ttcatggatg ttgtgtactc
    22861 tgtaattttt atctgtctgt gcgctatgcc tatattggtt aaagtattta gtgacctaag
    22921 tcaataaaat tttaatttac tcacggcagg taaccagttc agaagctgct gtcagagact
    22981 ctttttttaa tccacacaga gacatattgc ccgttgcagt cagaatgaaa agctgaaaaa
    23041 tacttactaa ggcgtttttt atttggtgat atttttttca atatcatgca gcaaacggtg
    23101 caacattgcc gtgtctcgtt gctctaaaag ccccaggcgt tgttgtaacc agtcgaccag
    23161 ttttatgtca tctgccactg ccagagtcgt cagcaatgcc atggctcgtt cgcgtaaagc
    23221 ttgcagttga tgttggtctg ccgttgtgtc acttttcgtc ggttgttgta ttaatgttgc
    23281 taattgatag caatagacca tcaccgcctg ccccagattg agcgaaggat aatccgccac
    23341 catcggcaca ccagtaagaa cgtcagccaa cgctaactct tcgttagtca gcccggaatc
    23401 ttcgcgacca aacaccagcg cggcatggct catccatgaa gatttttcct ctaacagcgg
    23461 caccagttct actggcgtgg cgtagtaatg atatttcgcc cgactgcgcg cagtggtggc
    23521 gacagtgaaa tcgacatcgt gtaacgattc agccagcgtc gggaaaactt taatattatc
    23581 aataatatca ccagatccat gtgcgaccca gcgagtggct ggctccagat gtgcctgact
    23641 atcgacaatc cgcagttcgc taaaccccat cgttttcatt gcccgcgccg ctgccccaat
    23701 attttctgct ctggcgggtg cgaccagaat aatcgttata cgcatattgc cactcttctt
    23761 gaccaaatga ccgcgaacag ggtgatcact gtcaacttat tacgcggtgc gaatttacaa
    23821 attcttaacg taagtcgcag aaaaagccct ttacttagct taaaaaaggc taaactattt
    23881 cctgactgta ctaacggttg aattgttaaa aaatgctaca tatccttctg tttacttagg
    23941 ataattttat aaaaaataaa tctcgacaat tggattcacc acgtttatta gttgtatgat
    24001 gcaactagtt ggattattaa aataatgtga cgaaagctag catttagata cgatgatttc
    24061 atcaaactgt taacgtgcta caattgaact tgatatatgt caacgaagcg tagttttatt
    24121 gggtgtccgg cccctcttag cctgttatgt tgctgttaaa atggttagga tgacagccgt
    24181 ttttgacact gtcgggtcct gagggaaagt acccacgacc aagctaatga tgttgttgac
    24241 gttgatggaa agtgcatcaa gaacgcaatt acgtacttta gtcatgttac gccgatcatg
    24301 ttaatttgca gcatgcatca ggcaggtcag ggacttttgt acttcctgtt tcgatttagt
    24361 tggcaattta ggtagcaaac atgcagaccc cgcacattct tatcgttgaa gacgagttgg
    24421 taacacgcaa cacgttgaaa agtattttcg aagcggaagg ctatgatgtt ttcgaagcga
    24481 cagatggcgc ggaaatgcat cagatcctct ctgaatatga catcaacctg gtgatcatgg
    24541 atatcaatct gccgggtaag aacggtcttc tgttagcgcg tgaactgcgc gagcaggcga
    24601 atgttgcgtt gatgttcctg actggccgtg acaacgaagt cgataaaatt ctcggcctcg
    24661 aaatcggtgc agatgactac atcaccaaac cgttcaaccc gcgtgaactg acgattcgtg
    24721 cacgcaacct gctgtcccgt accatgaatc tgggtactgt cagcgaagaa cgtcgtagcg
    24781 ttgaaagcta caagttcaat ggttgggaac tggacatcaa cagccgttca ttgatcggcc
    24841 ctgatggcga gcagtacaag ctgccgcgca gtgagttccg cgccatgctt cacttctgtg
    24901 aaaacccagg caaaattcag tcccgtgctg aactgctgaa gaaaatgacc ggccgtgagc
    24961 tgaaaccgca cgaccgtact gtagacgtga cgatccgccg tattcgtaaa catttcgaat
    25021 ctacgccgga tacgccagaa atcatcgcca ccattcacgg tgaaggttat cgcttctgcg
    25081 gtgatctgga agattaatcg gctttaccac cgtcaaaaaa aacggcgctt tttagcgccg
    25141 tttttatttt tcaaccttat ttccagatac gtaattgatc gtccattgta acgtctttac
    25201 tggctttcat tttcggcagt gaaaacgcat accagtcgat attacgagtc acaaacatca
    25261 tgccggccag cgccaccacc agcacactgg ttcccaacaa cagcgcgcta tcggcagagt
    25321 tgagcagtcc ccacatcaca ccatccagca acaacagcgc gagggtaaac aacatgctgt
    25381 tgcgccagcc tttcaatacc gcctgcaaat aaataccgtt cattaacgcc ccaatcagac
    25441 tggcgattat ccatgccacg gtaaaaccgg tatgttcaga aagcgccagc aagagcaaat
    25501 aaaacatcac caatgaaagc cccaccagca aatattgcat tgggtgtaaa cgttgcgcgg
    25561 tgagcgtttc aaaaacaaag aacgccataa aagtcagcgc aatcagcaga atggcatact
    25621 tagtcgcccg gtcagttaat tggtattgat cggctggcgt cgtcactgcg acgctaaacg
    25681 cggggaagtt ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg agattattag
    25741 caaaccagct gctttgccat tgcgcctgaa aacctgactc actaacttcc cgtttggctg
    25801 gtagaaaatc acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc atttcgctat
    25861 tacgccccgc aggcaccaca gaaagatcgc cggtaccgct taaattcagg gccatattca
    25921 gcttcaggtt ctgcttgcgc cagtcccctt cgggtaaagg gatatgcacg ccctgcccgc
    25981 cttgctctaa cccggtgccg ggttcaatgg tcagcgccgt tccgttaact tcaggcgctt
    26041 tcaccacacc aataccacgc gcatccccga cgctaatcac aataaatggc ttgcctaagg
    26101 tgatatttgg cgcgtcgagt tcgctaagac gcgaaacatc gaaatcggct tttagcgtta
    26161 aatcactgtg ccagacctga ccggtataaa tccctatctt gcgttcttcc acgttctgat
    26221 tgccatcaac catcaatgac tcaggtaacc aaaaatggat aaaacttcgt ttccgctcca
    26281 cggttttatc ctcttcctgc accgtataaa gctcggtcac aggaatagcg ataagcggcc
    26341 caacgagttt ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca tcgctacggt
    26401 aatcggcacg ttcgacaatc acctgccgaa tcagcattat cggaatcaac aacagcaata
    26461 ctgcaccaaa cagagtggtc attttccaga acagggggga tttcaacata gtcttctcct
    26521 ttgcaatggc agcaggatac gaaggctatg tgggaagaat ttgaagctat gtgaagtgac
    26581 ggtgaagtcg aagcgaggcc agcacgccgc cttcctgcac gttgcgcagc gtgacttcgc
    26641 cgttaaacaa acgggcgacc tcactgacga acgccaaccc cagaccgctg cttttttgcc
    26701 catttgcacg cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg tagtaaggaa
    26761 taccactacc ggtatccagc accttaagcg tgacgtgttc atgatccact tcggcgctta
    26821 gcgtgatgca accgctctcg ggagtaaaat cgatggcgtt atccagtaaa ttccccagcg
    26881 cctgctccag taacgccggt tcagcagcaa cgttaacctc agtaggcata acatgcaaag
    26941 tgatgttttt ttctgccaac tgcacggtgc gcgcttcgct gacgcggcga aataatgccg
    27001 ccacatcaac agcagtcaga acgacttcct gacgattctc cagtcttgcc tggcgtagta
    27061 acgtttccac cagcgcctgc attcgcgcat tttgcgtcag aatgttgtca gtaaaacgag
    27121 ctaccacttc cggcggcgga ccttcgcgta aaatttccgc cgcgccacga atcgccgcca
    27181 gtgggctttt tagctcatgg gttaacgcat aaacatactg ctcaatatag tttttccctt
    27241 ccagcttcac gcgcatactt tccagcgcct gcgcaagctt acgcaactcg ctactgccga
    27301 gttcaggaag aggaacgggc ttattgtcag tgacggaatc ggcatagcga gtgagccggg
    27361 caatggagcg gttgatccac caaaccatgc ctgcgccaat caccagtgca atccccagca
    27421 aaatggcgct ggcccataaa attcgccgct cgctgcgctt aatgaccgga gccatcgccg
    27481 cgttcggttt gcctacgctc aaaacgccaa taagccgcga gccgtccata atcggcgcgg
    27541 caacatacat caccgaactt tcgggatcgg caggattttg caacgtgctg cgcgcaccat
    27601 actgaccacg caacgttagc cagacgtcat tccagcgcga ataatcctgc ccaacggctt
    27661 tatttgccga atcgaacaat actttgccct gcgcatcggt catatagaca tgatattcat
    27721 tgcgcacttt gttaatgcca ccgatattgg cgcgaaacgg gcgatgctgt agctgattaa
    27781 acgcctgcgc cagttgccca tgcgttgggt ccccagagag caaatcggga cgcgccagct
    27841 ccgccagcaa cgttgcggta tcgattaacg tcccctccgt tgctcttcgc acgcccggtt
    27901 taacttcttt gacaaaaata gccagtacga accaggccgc caccgccacc agtaaaaaat
    27961 agcccagcag caaccgcatg ccgatacgca ttacaggccc ctcaggctat atcccatgcc
    28021 gcgatgagta ttaatcggag aaagatcggg gttgatggcg cgcagcttgg cacgcaacgt
    28081 tttaatgtgg gtatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc acacgctatc
    28141 cattagttgc tggcgggacc agacgcggcc tggcgacttg agtaacgtct tcagcaataa
    28201 aaactcatac cgcgtcagcg ttaatggcgt gtcaaaccag ctgatctgcg ccgcgggttc
    28261 attcaattca aaatgaccaa tacggatgac gggagacggc gacgagaact tcttcacccg
    28321 acgcagcaag gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt tagccacata
    28381 gtcgtcagcg ccaatttcca gcccaagcag gcgatcgacc tcttcacttc gggccgtcag
    28441 gaacagtaca ggtaacgccg gatggagcgc cagtaactgg cggcacaatt caaagccact
    28501 gatatcaggc aaaccaacgt cgagaatcat gacatcggga acctgctggc gagctttatc
    28561 cagcaccggc aagcctcgct caaagacctc gacggcaaaa ccttcctgct gcaacatgta
    28621 gaccagcgtg tcggctatcc cttgctcatc ttccactaac cagaccgttt cccgttgcat
    28681 aaatcgcctc tgttattgtc gccacggcat gacaggaacc gcgctaatcg cgtttttcgg
    28741 cgaaccttct acaactttgt cggagtaagc cagataagcg agcgcgttgc gtttggcgtc
    28801 ataaaagcgc acgacctgta acgacttaaa gaccagggac gtgcgttttt tgaatactac
    28861 ctcgccctga gctttgccgt ttttaatacg atccgacagt tcaatcggcc cgacttgctg
    28921 acaagaaata gccgcatcgg aggtatcttc cgccagaccc aatccccctt taataccacc
    28981 tgttttcgcc cggctcacat aacaggtgac atttttcaca tcgggatcgt caaaggcttc
    29041 cacaacaatt ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg aaccaatctc
    29101 ttctgcatga gccaatggcc ccagcattat caggcttaaa gacaggatca aatgcttgta
    29161 tttcatattg ttaccatttt tatctattac acgagcaatt agttcgtcac ggtaaataac
    29221 atattgatta agaacaaaat ctcaatactt ttatttccgt caagacctaa aacatactct
    29281 actaagaaaa aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca attacctgat
    29341 gtcaggtgct cgttgttgaa aggatgagga tattttatgg atcaggccgg cattattcgc
    29401 gaccttttaa tctggctgga aggtcatctg gatcagcccc tgtcgctcga caatgtagcg
    29461 gcgaaagcag gttattccaa gtggcactta cagagaatgt ttaaagatgt cactggccat
    29521 gctattggcg cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc actacgcctg
    29581 actgcgcgtc cgattctgga catcgcgctg caataccgct tcgactctca acagacattt
    29641 acccgcgcat tcaagaagca gtttgcccag actcctgcac tttaccgccg ttctcctgaa
    29701 tggagcgcct ttggtattcg cccgccgctg cgtctgggtg aattcaccat gccagagcat
    29761 aaatttgtca ccctggaaga tacgccgctg attggtgtta cccagagcta ctcctgttcg
    29821 ctggagcaaa tctctgattt ccgccatgaa atgcgttatc agttctggca cgattttctc
    29881 ggcaacgcgc cgaccattcc gccagtgctc tacggcctga atgaaacgcg tccgagtcag
    29941 gataaagacg acgaacaaga ggtattctat accaccgcgt tagcccagga tcaggcagat
    30001 ggctatgtac tgacggggca tccggtgatg ctgcagggcg gcgaatatgt gatgtttacc
    30061 tatgaaggtc tgggaaccgg cgtgcaggag tttatcctga cggtatacgg aacgtgcatg
    30121 ccaatgctca acctgacgcg ccgtaaaggt caggatattg agcgatacta cccggcagaa
    30181 gacgccaaag cgggagatcg cccaattaat ctgcgctgtg aactgctgat tccgatccgt
    30241 cgttaacgct gcagctcatc taatgcaggg gcgtccagat gcgagatgtc ccctgcggtt
    30301 tcaacgaccc agccagacgc cagccacagg ctttcctgat aatccacgcg cgaaatagaa
    30361 cagttacgca gacgtaagcg gcgctctgcc catgctggta atccgagaat cgtactcacc
    30421 aggcatccca gtgcaatacc atggctcacc agcagcggtc ggcttccctg cggtaagtcc
    30481 cggcaagatt ccagagccgc attgacgcga tcgctgagct cctgcattga ctcgccttca
    30541 ggaatacgcc cgtcaacggt gccattgacc agctgccgac gccagttctc ttcttcttcg
    30601 gtcagagaat cgatatgtct tttttccagc acacccatgt ttaattcacg caggcgagaa
    30661 tcaaagatga tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg ccgggtgcgt
    30721 cctaaatcgc tactgatgat atgcgtaatg ccaagctctt tggcacgggt tgccacctgc
    30781 atcgcctgtt gctcaccttt ggcggtcagc gggctgtcag actggccctg aatacgtcgc
    30841 tcggcgttcc actgcgtttc accgtggcgg acaaggtata cctgtaacat gctttttttc
    30901 cgtatactgc tgtaacgtta attttctgag taatgctgat tatgcaccaa gttgtctgtg
    30961 cgaccaccaa tcccgctaaa attcaggcca ttctgcaggc atttcacgag atcttcggcg
    31021 aaggatcctg ccatattgca tccgttgccg tcgagagcgg tgtaccggaa cagccctttg
    31081 gcagtgagga aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc cgtttacttc
    31141 cagaggctga tttttgggtg gcgattgaag ctggcatcga tggtgacagc actttcagtt
    31201 gggtggtgat tgaaaacgcc agccagcgcg gcgaggcgcg ttcggctact ttgccgttgc
    31261 ctgcggtgat tctggaaaaa gtgcgtgaag gtgaggcgct tggtcccgtg atgtcgcgtt
    31321 ataccggtat tgatgagatt ggtcgtaagg aaggggcgat cggcgtgttt accgccggga
    31381 aactcactcg cgccagcgtc tatcaccagg cggtgatcct ggcgctgagt ccgtttcata
    31441 atgccgtgta ttaagcgcct gatgcgacgc tgccgcgtct tatcatgcct accaaacata
    31501 ttgaaattac gggtatttgt aggacggata aggcgttcac gccgcatccg gcattcggtg
    31561 cacgatgcct gatgcgccac gtcttatcag gcctacaaaa tcaatcgctt ttcagcaaca
    31621 cctcttccag ccactggcgc agctcaacgg gcgcggcttt caggctgtta gatccacgcg
    31681 taatcgtcgc gatgcccgcg ccgagttcat tttttaactc acgctggctc atttcgccgc
    31741 gcaacagctc ttcgacaata cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca
    31801 gcatcaggtt taacaacggt aaatggagat cgttttggta ggcattctta agcaggtcga
    31861 caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt
    31921 gggccataat atgtcgccat tgttagcggg ggaagcaaaa tgcctccccc ggttgtactc
    31981 gctaaagagt acgatagcat atcataaacg tgcggatcag taacgacgtc cccattccgt
    32041 ggcactcatc aacgtcggtt tatcccccat gaaatagcgg tagtaagcgt cataagccag
    32101 cacgttcttc acataaccgc gcgtctcgga gaatggaata ctctcgacaa atgccactgc
    32161 gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc cgccctggtc cggcgttata
    32221 agctgctgag gagaaaatac gattattgcc aaactgctga taaacatatt gcaggtaact
    32281 ggtgccaatg ttgatattcg tttccggatc cagcaattgc ccaggactgc tataaccggg
    32341 aatagagaac atcttcaccg tatgggtcgc tgtaccaggc ataatctgca tcaggccgct
    32401 ggcccctacc ggtgatttca ctttcggatt ccaggcgctc tcctgacgag caatcgccat
    32461 cgcatagctt tgcgggatct ccttaccgct ggtgtagcgt ttgaaaagat cgttgtaagc
    32521 cagcgggaat cgctcttcca gatgatccca cagcttcccg gcgatcgttg cctgaacgct
    32581 aagatcccac cattggttgt tgaaagcata ccgcgccagt tgagcttgct ctgtttttga
    32641 cttgctcttc accagattgg cccactcgct acgcgcggtg ttatcgagat tccagtacat
    32701 caactcgcgc acgcgcgcca tctccggccc ctcagtcagg gcgctgtcaa cattctgcgg
    32761 cgctttatca atcttcagct catactcttc gccgatgcgt tgtgctgcaa ccatcgggta
    32821 gaaaccacgc tgttgcatga gttgatgcaa aatctctttt gcttcagctt cacgtccgcg
    32881 ctccagcaat aaatccgcct gccagtaacg ccattcatct ttctctttcg cctccatcgg
    32941 caaacgcgcc agccaggtat tcaggccgcg acgatcgcca gtgccaagcg ccattcgcac
    33001 acggcgttca ataagcgaag tagattgcga gcgcataatg gcgtcatcgc gccatttcgc
    33061 ctgctcgtcg gtgacatcgt tgcccatcaa acgccaggcg acgatatcgc gcagctcctg
    33121 aatctgatct tcattaagct gctgcgcctg ggcaagcgat gggatcatca gccgtgcatt
    33181 ttcagcatcc tgccgcgcca cactggcaaa cgccaccgcc gccatttgac gggtaaaatc
    33241 ggtcgctccg gttgtacgcg cgaaggtcag taccgtatta gggttgttcg ccagtgaaat
    33301 gattgccgag gcgatagtct ggtaatcggc aggcatctgc cctgccagca ctgttaccag
    33361 gccagtgtta cccgctttca tcgccagacg gatacgctct aaatacgcca gcggatcttg
    33421 tttacctgac gcacgccaga cactaaataa cttgtcacag gcgttaggct ggctcttgcc
    33481 ggttagccac agctctttcg ccccttgcca ggcttcttca ctctgcccgg tgttccattt
    33541 cgcatagtag taattacatt gtgcttcggt agctccgggc ttttccgggc taaaggctaa
    33601 caggccacgc cagtcttcac gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg
    33661 agcgggagga agcgtggggt tagcgcgaac aaagttagtg accgtcaccg ccggttgatt
    33721 catcaggtca tcggtgatct ggcggtattc caggtaggga taaagcggat aatccttcag
    33781 tccaggcatc atttgttcga ccacatccat ttgtcgatta tcccaggcct gcttgatttg
    33841 cgcgtaacga ctacgctgct catccagtga gtcggctcgc gccacgctgc tgaccgtcag
    33901 cagacagaca ccggcagcca acagccgcca ggtaacttgt ttggcttttt ccacaagcgc
    33961 atcctctaag tgtaaataca tcaatgcagc gtcatgccgc gtaatagatt tatgctaacc
    34021 agtcattgcc gtttacgcca cgttacggac acttttttac ttttactgcg aaggtgatcg
    34081 accaactggg attaggcggg gaaaaaggct acacttcgcc tttgaaaact catcatcacg
    34141 ataaacatag aggcgaagtc caacgtggct caattcgttt ataccatgca tcgtgtcggc
    34201 aaagttgttc cgccgaaacg tcatattttg aaaaacatct ctctgagttt cttccctggg
    34261 gcaaaaattg gtgtcctggg tctgaatggc gcgggtaagt ccaccctgct gcgcattatg
    34321 gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc agccagacat caagattggt
    34381 tatctgccgc aggaaccgca gctgaacccg gaacacaccg tgcgtgagtc cattgaagaa
    34441 gcggtttcag aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc gctgtacgcc
    34501 gatccggatg ccgattttga caagctggcc gctgaacaag gccgtctgga agagatcatt
    34561 caggctcacg acggtcataa tctgaacgta cagctggagc gtgcggcgga tgcgctacgt
    34621 ctgccggact gggacgcgaa aatcgctaac ctctccggtg gtgagcgtcg tcgcgtggcg
    34681 ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc tcgacgaacc gaccaaccac
    34741 ctggatgccg aatccgtggc ctggctggaa cgcttcctgc acgacttcga aggcaccgtg
    34801 gtggcgatta cccacgaccg ttacttcctc gataacgttg cgggctggat cctcgaactt
    34861 gaccgcggtg aaggtattcc gtgggaaggt aactactcct cctggctgga gcagaaagat
    34921 cagcgtctgg cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag
    34981 ctggagtggg tacgtcaggg aactaaaggc cgccagtcga aaggtaaagc acgtctggca
    35041 cgctttgaag agctgaacag caccgaatac cagaaacgta acgaaaccaa cgaactgttt
    35101 attccacctg gaccgcgtct gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc
    35161 tacggcgatc gcctgctgat tgatgacctg agcttctcga tcccgaaagg ggcgatcgtc
    35221 gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt tccgtatgat ctccggtcag
    35281 gaacagccgg acagcggcac catcactttg ggtgaaacgg tgaaactggc atcggttgat
    35341 cagttccgtg actcaatgga taacagcaaa accgtttggg aagaagtttc aggcgggctg
    35401 gatattatga agatcggcaa caccgagatg ccaagccgcg cctacgttgg ccgctttaac
    35461 tttaaagggg ttgatcaggg taaacgcgtt ggtgaactct ccggtggtga gcgcggtcgt
    35521 ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc
    35581 aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg ccctgctgga gttcccgggc
    35641 tgtgcgatgg ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac gcacattctg
    35701 gattaccagg atgaaggtaa agttgagttc ttcgaaggta actttaccga gtacgaagag
    35761 tacaagaaac gcacgctggg cgcagacgcg ctggagccga agcgtatcaa gtacaagcgt
    35821 attgcgaagt aatgcgtaaa atgccggatg cggcgcgcca gcaccgcatc cggtattagt
    35881 taaatgatta gtccccggaa aaaggcagaa ttttctttta gtgtattagc tcagatgcgg
    35941 actgacatct caaatcaagc gggaactcat gattcccgct taacgttcta ttcaccgcgc
    36001 agaatctggc ggaaatggga aaccaacgga cgttggttgg ctttatgcca ggctgcccat
    36061 aattgcgttg aataattcat ccacggcaat tcacgtaata ccaccccttc cggcgcatta
    36121 tgacgcagac tatgttgaat taaggccaga cctaatcccg cagcgaccag acctaatgcc
    36181 gtgaggggtt cacccgcttc caggcgaata tcaggattaa acccggcgtt aacacaggcg
    36241 gcgacaaaat gatcgcgaga atgcgcatcg tcgttatgaa tcaccgcaat ccactgctca
    36301 tcgccaagtt gtgacgcgac aaggttttcc tcctgcgcca gaggatgatt ttgcggtaag
    36361 gccaacaaca ttcgatcatc cagaacctga atatattcca gatcctgctc gctgtcggtg
    36421 ggcggcgtat cgaccagaga aaaatcgaga ctgcgctggc gcaggccttc aatctgggta
    36481 gccggtggtt ggctgtaaag tgcgatatgc acattcggac gcgatacacg taacgtacgt
    36541 agcgccgtag aaagcacacc ggaatgcatg gcgttttcga tatagccaat acacaagcca
    36601 ccatcttccc cccgtcccag gcgtttgcct aacgactcca ggcggttggc gtgtgtcagc
    36661 aacgaacgtg tttcggcaag gaatgtctgt ccatcagcgg tcaggcgaat gcgctgctga
    36721 ttacgctcaa acagcgtcag ccccagtcgt tcttccagtt gtgcaatctg gcggctcagc
    36781 ggcgattgcg agatatgtaa tcgctcggct gcgcgtccca cgtgctcttc ttctgcgacc
    36841 accacgaagt acttcagttg gcgtaaatca atcatgttag acctcaaagg acttaagtca
    36901 gacgaattat gtcctatcaa gtcacaacct cgcaaggtat tgtgatccac gtcacagggc
    36961 gatagcaaaa aaacgcccta acccaaaccg aataattgag gacatcttat gagcattaaa
    37021 aatttactcc ccggcaaaat tggcttaggc ggcgcaccac ttggcaacat gttccgtgcc
    37081 attccggaag aagaggccca cgccaccgtg agcgaagcct ggaacctggg tgtgcgttac
    37141 tttgataccg cgccgctgta cggctctggc ctttctgaaa tccgcatggg tgaagcgctg
    37201 tctcagtatc cacgcgatga atacgttctg agtagcaaag tcggtcgcat catgctggac
    37261 gaaatggaag atccggcaaa acgggatttc ggtgaaaaag gtggcctgtt cgaacacggc
    37321 ctgaaaaaca aaattctgaa tgactactct gctgacgcca ctctgcgctc gattgaagac
    37381 agcctgaaac gcctgaaaac agaccgtctg gatattgtct ggatccacga cccggcgcag
    37441 gacttctacg gtgacagctg gctggagcag ttcaacattg cccgtaccgg tgcatttcgt
    37501 gcgctgacgc gtttgcgtga cgaaggtgtg attaaagcct ggggactggg tgtgaaccgc
    37561 gtcgaacctt gcgaactgac gctggcgctg gacgaaccga agccggatgc gttcctgctg
    37621 gcggggcgtt attccctgct ggatcacgaa cgtgcgctgc aacgcctgat gccagaagca
    37681 ctggaacaga acgtggatat cgttgtgggc ggtccataca gctctggcgt actggcaggc
    37741 ggcgagcact ttgaatatca gaaagcctct gcggccatcc agcagaaagt ggcgcaaatt
    37801 aacgccattg ccacacgttt caatgtgaac gttaaagcgg ctgcgctgca gttcgcgctg
    37861 gcaaacccgg ccgttgcagc ggttattcct ggctccagcc gtcctggtcg tatggcagaa
    37921 gatctggctg cactgaatgc gcctgttccg gctgaattct gggcagaaat gcgccgtcaa
    37981 aatctggttg cggaaaacgc tccgctgccg acccgttaat caaccaggtg agaaaacgat
    38041 gaaactggat ctgacaggta aagtcgcggt ggtcagcggc tcaacctccg gcattgggca
    38101 gggtatcgcc gccggactgg caaaagcagg cgcaacggtg gttgtcgtcg gccgtaaaca
    38161 gagcggcgtt gatgacgcca tggctgcgat tgcggcacaa actcccacag cgtcactgcg
    38221 cggcgtggtg gccgatctca gcaccgaaga cggcgtggct gcgctgatcg ctgcagtccc
    38281 aacggctgat gtgctggtca ataatctggg catttttaac gataaagatt tctttagcgt
    38341 tccggatgat gagtggatgc acttctataa cgtcaacgtc ctctccggcg ttcgtctggc
    38401 gcgtcactat gcgccaggca tgacggaaca gggctggggg cggattatct ttgtctcgtc
    38461 cgaatccggc gtggcaatcc ctggcgacat gatcaactac ggcgtcacca aagccgcgaa
    38521 tctggcggtt tctcatggtc tggcgaaacg actggcgggg accggtgtga ccgtcaacgc
    38581 cattctgcca gggccaacct ttaccgatgg cctgaaagag atgctggcgg atgccgctgc
    38641 acaatcggga cgcagtgcgc gtgaacaggc cgatgaattt gttaaaacct cgcgtccgag
    38701 ctccatcatc cagcgtgcgg cagatgtgga agaagtggcg aatctggcgg tttatctcgc
    38761 gtcaccgctt tcctcggcaa ccacaggggc agccctgcgc gtcgatggcg gcgtcgtcga
    38821 caccctgaca atgtaaccct ataaattaag agaaaattat catggctaaa acaactgtat
    38881 cgatcgaaat tccggcttct gccgacgccg tatggcaatt gatgggcggc tttgatgcgc
    38941 tgccagactg gctaccattc atcccgaaaa gcgtggtgac agaaggcggt cgcgttcgtt
    39001 cgctgacaac ctctgacggc ggcactgtgg ttgaacgtct ggaagcgttt gataaccgtc
    39061 aacgcagtta tacctattcc attattcagg caccgttccc ggtggtggat tacctctcca
    39121 ccattaccgt tcatgaaacc gccgacagcc aggtttcccg tgtggaatgg ttcggtgaat
    39181 tcaccccggt gaatgtgacg aacgaagagg ccgaggcgct gtttaccggc atctatcggg
    39241 atggactcaa ggcgcttaag gacaactttg ttgcctgatg cgatcgacag gcgtcggcac
    39301 gtccggcgtc tgattattaa caaaaacaga atagtctatt gtcatttgta tcatttatgt
    39361 tatcagacag caggcgtatt ctctccccat cgcgttaata aacgccaaac aattaagaca
    39421 ctctggaata taaacatgaa aacgatcatc gcaattttta ccgcttctct tttctctgtc
    39481 gcggcgttta acgcatcggc gcaaagcgtg acagcaaccg gtacaacact ggattccgct
    39541 gaagcggtta ttgcgcagaa agcgaaagag atgaacgcct ctgaatacaa aattaccagc
    39601 gctcgcatgg gcaataccgt caccatgagt gccgaactgt ataaataatc gcgcgaggaa
    39661 cccattacgt aatttgctaa atatgcgctg aatgcgttgc cattaataaa ccgtattgta
    39721 ctgccaccga tgacgcgttc ccgcgccggt gcgggtgacg ttgcgattga catgatggcg
    39781 gagtattacg cgcagcgcgc cagtgctgga cttatcatct gcgaaggtac gcaaatcagc
    39841 agaagcgctg cgcataattt tccacggcac gctgatttac tccggtaaat acaccaaagc
    39901 gcgcgccgaa gcgaactgtc agatttgatt gcattctggc tacgcaaccg gtcggttgga
    39961 gtatgaagtt gctcacgaat agccttagca tccgcaattt cgtagcgggt cacccgtaca
    40021 ggttctttct aatctcgaca gtctcttcca gagaggtttt caactcatca aaaaaactca
    40081 ttttatacct cgcctttgag cagtttcgtc acggttatct ctgctccccc atcatctccc
    40141 gcaccagctc cacgcagcgc aggaaacggc tgtcgtaatc ttcctcttca acccgcacaa
    40201 attcgatatt gttctcttcg agcatctcca ccagcaagtt ctggaactct ttgcgatcca
    40261 ccgaactgcc gaggctgcgt aaaccatccg ccacccacgg cgtgttgttt tccagcagga
    40321 tcaccagatc aaaacggtat tcatcaatca gcgcctgcac gaacggatgc tcgcgtccct
    40381 cgtacttttt gcagaacgcc tgagtggtga caaaatcggt atcgataaat gccactttat
    40441 tagcatattt caccgcaaaa tcaatgtatt gagcgtggcc cagcgcgatt ttatcgtagt
    40501 cggaatactg caatgcgatc tcatcaccgc cgaggtgtga aaagacataa tcgcgaccat
    40561 attcccacgc actggtggta ttgaagatat tggcaagttt gtttaccagg gtggatttac
    40621 cgctcgactc gccgccaagg atcgccacgg tacgcacaaa gaacggcttc acttccgtag
    40681 gaatatattc ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg atactcataa
    40741 aggtacgttt cggatcgacc agcaccgtct cgatccccag atgttccata tactgtggcg
    40801 catcggcttc ttccgaggtg tagatcagat ccggctggat ccctttttca gccataaact
    40861 ttttgatacc gttgctccac acatcccagc cgtgcggata cggctccatg ccctcttcgt
    40921 tgaaagcatg aatgcgaata tttttctgat atttaaaagt ttgcaataac cagcgcagac
    40981 gatccggcac ggtcggctgc tgcgacatgg cactttcttc gaacaacgcg cggtcacggg
    41041 tatcgtcaaa acccataatg atatgcagct cgtcaacctg gctacaggcg cgctggataa
    41101 ggtagatatg tccggtatgc agtgggtaga acttaccgaa tacgacaccg atcgttttct
    41161 tctgccgggg aaactcaagc cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctgg
    41221 ggcttttgat tttggcattc agtaactggc ttaaataccc tttggtcata ccgctggcat
    41281 cagctacctg ctgtagcgtg cagccctgtt gcttgatggc agttttcagg taatcaaatg
    41341 acgacatatc tccctccgta tttctcatta taagtcgtcg aacacgctaa gcgcgtcgga
    41401 gagtttttta acgccaaaaa tctgcatccc ttccggcgct tttttcggca cgttagccgc
    41461 cggaacaatc gcccggcgaa aaccgtgttt cgccgcttca gagattcgtt cctgaccgct
    41521 gggcaccggg cggatctccc ctgccagccc gacttcacca aacaccacca gatcctgtgg
    41581 cagcggtctg tcacgcaggc tggaaaccat cgccagcagt aacgctaagt cggcgctggt
    41641 ttcggttacc ttcacgccgc cgaccacgtt cacaaacaca tcctgatcgg ccatttgcag
    41701 accaccgtga cggtgcaaga cagccagcag gattgccaga cggttttgtt ccagccccac
    41761 tgccacgcgg cgcggattcg ccatcatcga gtgatcgacc agcgcctgaa tctccaccag
    41821 caacggacgc gttccttccc acaccaccat cacggagctg ccggaggtca cttcatctcc
    41881 gcgacttaag aaaattgccg aagggttgct gacttcacgc agaccctgtt cggtcatcgc
    41941 gaatacgccc agctcattca ccgcgccgaa gcggttttta tggctgcgta aggtgcgaaa
    42001 acgggagtcg gcatcgccat ccaaaagcac cgaacagtcg atacagtgtt caagcacttt
    42061 cgggccagcc agcgaaccat ctttggttac gtgccccacc atgacaatcg ccacaccgcg
    42121 cgttttggcg aagcgtgtca aataagccgc cgtttcacgc acctgcgcca cgctaccagg
    42181 cgatgactgt acatccgcca tatgcatcac ctggatcgag tcaattacca tcagcttcgg
    42241 ttgctcttct tcggcaatca ggcagatctg ttcgatgctg gtttccgaca acatattgag
    42301 attgtcagtc ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca gcgactcttc
    42361 gccggtgaca tacagcgttt tcatctgctg ggccagtttg cacagcgttt gcagtagcag
    42421 cgtggatttc cccgcaccag ggttaccgcc aatcagaatg gcacttcctg gcaccacgcc
    42481 gccgcctaat acgcggtcga actctttaaa tccggtggaa aaacgcggca gctcttcaag
    42541 gctgatatcg gagagtttct ggacttttgc caccccggcg ctaccggcat agccgctgag
    42601 acgctcgtta cgcgccaccg ttggcgacgc agcaagacgc acctcggtga tggtgttcca
    42661 ggcatgacag gcactgcact gcccctgcca gcgcggataa tcggccccgc attcattaca
    42721 aacaaaggcg cgttttggag cttttgccac ggtgtacctc gttaatgctg tgccgcccgc
    42781 aggatggcgg gcgagcaatt acttctgatt caggctgcct gagaggatgc agaatacccc
    42841 catcaggtca gcgtgacgga tggtgacttc cgccttttca ttcacttttg gcttggcatg
    42901 gtaggcaatc cccagccctg ccgctttgat catcggcagg tcattggctc catcgccaat
    42961 cgccacggtc tgcgccagcg ggatttcata ctcctgcgcg aggcgagtca gagttttcgc
    43021 tttgtactgc gcgtctacga tgtcgccgat cacattgccg gtaaatttac cgtccatgat
    43081 ctccagttca ttggctacca cggctgtcag gcgcagcttg tcgcgcaggt attcagcaaa
    43141 gaaagtaaag ccgccggagg caatcgccac tttccagccc agcgtttcca gcttgagtac
    43201 cagctgcgtt aagcctggca tcagcggcag attttcacgc acctgttgca ggatattggc
    43261 gtcagcgcct ttcagcgtcg ccacacggct gcgcaggctg gcggtaaaat cgagttcgcc
    43321 gcgcatcgcc cgttcggtta cttccgccac catctcgccc gttccggcca gtttggcaat
    43381 ttcatcaata cattcaatct ggatggcggt ggaatccatg tccatcacca gcaaacccgg
    43441 cgtgcgcagg tgcgggattt tccccagcgg ggcgacatcc agttgcgctt catgggccag
    43501 gcgcgtagcc cgtgcggtga gtgaacctgc cagacgaatc acctgataat cttccacgca
    43561 ccaggcggca acaatcacca tcgccgcacc cagtttgctc tggtattggg tcagacgctg
    43621 tttatccagc ccacgaccat acagcagcca gccgctacga cctgcgtggt aatccagtgg
    43681 catcacttca tcaccactta atgaaagagg cagacccggc cataaagaga catcttcagg
    43741 caggtcgcac caggtaatgt taggcattaa ggctcctgta aaatcgttcg aagcagggaa
    43801 aataacgcat gaggctacct tgtatccatt gcttctggca acattaagtc tcaaattttc
    43861 aaagggtgga agatggctcg cacaaaactg aaattccggc tgcatcgggc agtgattgtc
    43921 ctgttctgtc ttgccttgtt agtggcactg atgcaggggg cgtcatggtt tagtcaaaac
    43981 caccagcgac agcgtaatcc acagctggaa gaactggccc gcaccctggc gcgtcaggtg
    44041 acgctgaacg ttgcaccgct gatgcgtacc gactcaccgg atgaaaaacg cattcaggca
    44101 atcctggatc agttaacgga tgaaagccgt atcctcgacg caggcgtgta tgacgaacaa
    44161 ggcgatctta tcgcccgttc tggcgaaagc gtcgaagtgc gcgaccggct ggcgctcgac
    44221 ggcaaaaaag cgggcggcta ttttaaccag cagattgtcg agccaattgc gggtaaaaac
    44281 ggaccgctcg gctatctgcg cctgacactc gacacccata cgctcgccac cgaagcccaa
    44341 caggtggata acaccactaa cattttacgc ctgatgttgc tgctctcact ggcaatcggc
    44401 gtagtgctga cccgcacgct gctacagggt aaacgcaccc gctggcagca atcgcccttc
    44461 ctgttaaccg ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc
    44521 attactacaa gaaaggaaat cgttatgtcc acattacgcc tgctcatctc tgactcttac
    44581 gacccgtggt ttaacctggc ggtggaagag tgtatttttc gccaaatgcc cgccacgcag
    44641 cgcgttctgt ttctctggcg caatgccgac acggtagtaa ttggtcgcgc gcagaacccg
    44701 tggaaagagt gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt
    44761 agcggtggcg gcgcggtgtt ccacgatctc ggcaatacct gctttacctt tatggctggc
    44821 aagccggagt acgataaaac catctccacg tcgattgtgc tcaatgcgct gaacgcgctt
    44881 ggcgtcagcg ccgaagcgtc tggacgtaac gatctggtgg tgaaaaccgc cgaaggcgac
    44941 cgcaaagtct caggatcggc ctatcgcgaa accaaagatc gtggcttcca ccacggcacc
    45001 ttgctgctca atgccgacct tagccgcctg gcaaactatc tcaatccgga taaaaagaaa
    45061 ctggcggcga aaggcattac ctcagtgcgt tcccgcgtga ccaacctcac cgagctgctg
    45121 ccggggatca cccatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat
    45181 ggcgagcgcg tggaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc
    45241 gccgaaacct ttgcccgcca gagtagctgg gagtggaact tcggtcaggc tccggcattc
    45301 tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa
    45361 aaaggccata tcacccacgc ccaggtgttt accgacagcc tcaaccccgc gccgctggaa
    45421 gccctcgccg gacgactgca aggctgcctg taccgcgcag atatgctgca acaggagtgc
    45481 gaagcgctgt tggttgactt cccggaacag gaaaaagagc tacgggagtt atcggcatgg
    45541 atagcggggg cggtaaggta actcgctgat taaaatatcc aaggaaggaa atatgatcga
    45601 tctggaaaac caggaacggg agattatcaa tttaatgttc agccagcgta tttcctggct
    45661 agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa tgttaggtat
    45721 ctcgattaat tctctaaaac agatcgaaaa gacggaacgg cttagctcaa acatcaaaag
    45781 caaaatggca gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac
    45841 ggctgaacat aaatgaatag attcgtgaat agatacttga atgtatttta ttttctatcc
    45901 atatgaaaaa taatagtaaa tctataaatc agttgctatt gatgaataga tactaaaaag
    45961 ccggaacagt ctcccgctcc ggcttaacac aacaatcgcc tttcagcgaa acacaattac
    46021 tctttatcgc ccagcagaac ggattccagt gcgattttga tcatatcgtt gaaggtggtc
    46081 tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt ggtcagatac ggtgcagatg
    46141 gtcagggctt tcgcgccaaa ttctgcagcg acgccgtaga taccagccgc ttccatttcc
    46201 acgccgagaa tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac
    46261 aggtcagcgg agaacaggtt accaacgcga gcatcaatgc ccagtgcttt agccgcgtct
    46321 accgcgttac gcaccatgtc aaagtcagcg atagcggcaa agtcatgatc tttaaaacgg
    46381 atgcggttaa ctttggaatc ggtgcaggca cccataccga taacgacgtc gcgcagtttt
    46441 acgtgcggca gaactgcgcc acaggaaccc acgcggataa ttttcttcac gccgaaatcg
    46501 gtgatcagtt ctttggtgta gatggagcag gacggaatac ccataccgtg acccattacg
    46561 gaaattttgc ggcctttgta agtaccagtg aagcccagca taccgcgaac gttgttcact
    46621 tcacgggcat cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc
    46681 atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt agccattgtt
    46741 ttatccttat caacatgttt tcaccctctc cctgtgggag agggccgggg tgagggcatc
    46801 aagccgcacc cattatcaga acatggcttt gccatattcc atatcagaag taccaaaata
    46861 ttttgccaga gtctggccga tatccgcgaa ggtttcacgg tgacccagtg agcccggttt
    46921 tactttcggg ccgtaaacca gtaccggaat gtgttcacgc gtgtggtcag taccggtcca
    46981 ggtcggatca cagccgtggt cagcggtgag gatcaggatg tcgtcatcgc gcagcagaga
    47041 catcagctcc ggcagacggc ggtcgaacag ttccagaccc gcagcataac cggcgacgtc
    47101 gcgacggtgg ccccaggaag agtcgaagtc aacgaagttg gtgaagacga tggtgttatc
    47161 acccgcttct ttcatctctt tgatggtggc gtcaaacagc gcgtccaggc cagtcgcttt
    47221 cacttttttg gtgataccgc agttggcgta gatgtctgca attttaccga cagaaaccac
    47281 ctggccgtgt ttttcatcaa ccagtttctg cagcacggtc ggtgctggcg gctcaacagc
    47341 caggtcgtga cggttaccgg tacgctggaa gttaccggct ttgtcgccga taaacggacg
    47401 agcgataaca cgaccgatat tgtagccgcc gttggtcagc tcttcacggg cgatttcgca
    47461 cagttcgtag agtttatcca gaccgaaagt ttcttcatgg caggcaatct ggaacacgga
    47521 gtcagcggag gtatagaaaa tcggcttgcc ggttttcatg tgctcttcgc ccagctgatc
    47581 cagaatgacc gtaccggaag agtggcagtt accgaggtaa cccggcagat tagcgcgttc
    47641 aaccagttta tccagcagct cttgcgggaa gctgttttcg tgatcggaga aatatcccca
    47701 ctcaaacaga accgggacac cggcgatttc ccagtgacca gacggggtat ctttaccgga
    47761 tgacatttcg tgcgcccatg cgtacgcgcc gataacttca gcgttgccgt ccattcccgc
    47821 cggaatgaaa ccggtagaac cttcgtgtgc tttcgccagc cccagacggg tcagatttgg
    47881 cagattgagc gggcctttac gaccgttatc agcttcgcct ttggcacaag cttctgcgat
    47941 atgacccagg gtgtcagccc cgacgtcacc aaagcgttct gcatcttctg tagcgccgat
    48001 gccgaatgag tccagcacca taataaatgc acgtttcata tgttctccgt actcagtgct
    48061 tcaaatttaa atgatcagat cagtataccg ttattcgctg atacggcgat agacagttgg
    48121 tgtgctttcc ggtgctttat cggcaagttt aattgccgct ttcaccgctt tcgccgcttc
    48181 ctgccagctg ttttcgtctt tcgcgtggat aaccgccagc ggacgctgac cgtctacctg
    48241 gtcgcccaga cgtgccatat cagtaaagcc gacgctgtaa tcgatggtgt cagatgcctg
    48301 acggcgtccg ccgcccattg caaccactgc catccccagc gcgcgggtat ccatttcact
    48361 gacaaaacct tcggtatcag catagactgc tttcgtcagc atcgctgtcg gcagatactt
    48421 cgcgtagttc tcaacgaagt cggtcgggcc tttttgtgcc gctaccatac gaccaaagac
    48481 ttctgccgct ttaccgttgt ccagcaccgc ctgcaatttc gcgcgcgctt cggcgtcatc
    48541 tttcgccagt ttgccggaga tcagcatctc cacacacagc gccatcgtga catcaaacag
    48601 acgcgggtta cgatattcac ccgtcaggaa ctgcaccgct tcacgaactt caaccgcgtt
    48661 acctgcactg gaggccagta cctgattcat gtcggtgagc agcgcggtgg tgcgcacgcc
    48721 agcgccgtta gccacgccaa caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg
    48781 cataaacgcg ccgctaccca ctttcacgtc catcaccagc gcgtccagac cttccgcaag
    48841 tttcttcgcc agaatagagg cggtgatcag cgggatggag tccacggttg cggtaatatc
    48901 acgggtcgcg tagaaacgtt tatccgccgg agccagtgag ctggtctgac cgataatcgc
    48961 cacgccgacg tctttaataa tttcgcggaa acggttgtca tccgggaaaa tgtcgaagcc
    49021 agggatggat tccagtttgt cgagcgtacc gccagtatga ccgaggccgc gaccagagat
    49081 catcggaata tagccgccgc aggctgcgac catcggcccc aacatcagcg aagtcacatc
    49141 gccgacgcca ccggtggagt gtttatcaac aatcgggcca ttcagatgca ggcttttcca
    49201 gtcgagaacg gttcctgaat ctcgcatcgc catggtcagc gagacacgct caggcattgt
    49261 catatcgtgg aagaaaatgg tcatcgcgag ggcggcaatc tgcccttcgg agatagtgtt
    49321 gtcgcgaata ccgttgataa agaaacgaat ttcttcatcg ctcagcgcat gaccatcacg
    49381 ttttttacga ataatttctt gtgcgagaaa caaggtaacc tcccgaggaa aagagtatga
    49441 ggcggctttt gccggatgcg tctacgcctt atccgaccta cgggtagata cgtctcgtag
    49501 gcctgataag cgcagcgcat caggcgtaaa gcatcttact tagtagctgc tggcgctctt
    49561 accgtcgccg tgacccagcg ctttcagcag gcttgccagc aggctggaag caccaaagcg
    49621 gtagtgacgc gcatcagccc agtcagcacc gaacagttca tctgcgatgg cgagatattt
    49681 ctgcgcatct tccgcagtac gcacgccgcc cgccggtttg aaaccaacgg ttttttctac
    49741 gcccatatca cggatcactt ccatcatgat gcgcgcgctt tccggcgtcg cgttcacagc
    49801 cactttaccg gtagaggttt tgatgaagtc cgcacccgct ttgatggaga tttcagacgc
    49861 tttacggatc agcgcttcgt ctttcagttc gccggtttcg atgatcactt tcagcagtac
    49921 attcgctgcc gcgcaagcct ctttacaggc tttcaccagg tcaaaaccaa cctgctcgtt
    49981 acccgccatc agcgcgcggt acgggaacac aacgtcaact tcatcagcac cgtaggcgat
    50041 tgccgcacgg gtttctgcca gcgcgatttc gatgtcgtcg ctaccgtgtg ggaagttggt
    50101 taccgtagca atacggattt ccggggtgcc ctgctctttc agtgttttgc gagcaatcgg
    50161 gataaagcga ggatagatac agatagcggc ggtattgccg accggggttt tggcctgatg
    50221 acacagagca attactttct cgtcggtgtc gtcgtcattc agggtggtca ggtccatcaa
    50281 tttcagtgca cgcaggctgc ttgctttcag atcagtcatt tcattctcct ggcttgtcgc
    50341 caataaaatt caccttgcga gtttgttagt attctaacat ctactccgca acacacttcg
    50401 atacacatca caattaagga aatctactta caagtttgca tcactgtaat gcgatctggt
    50461 tcaaataatt cactttcaaa tgaatgcgtc agtggtggca aacgcatcag gatcaaagtg
    50521 aacatcacga aacttcttac aatggcgcat caccttcggc ataaaaggaa aagaagatgc
    50581 ccacttctca tgaaaatgca ctgcaacaac gttgccagca aattgtcacc agcccagtgc
    50641 ttagcccgga gcagaagcgc cattttctag cactggaagc agaaaacaat ctgccttacc
    50701 cacagcttcc tgccgaagcc cgccgcgcgc tggatgaagg tgtaatctgc gatatgtttg
    50761 aaggtcatgc gccgtacaaa ccgcgctatg tcttacccga ttacgcccgt tttctggcga
    50821 acggttccga atggctggag ctggaaggcg cgaaagatct tgatgacgca ctctctctgc
    50881 tgaccattct ttaccaccac gtaccgtcgg tcacatcgat gccggtctac ctggggcaac
    50941 tggatgcgtt gttgcaaccg tatgttagaa ttctaacaca agacgagatc gatgttcgaa
    51001 taaaacgttt ctggcgttac ctcgacagaa ccctgccaga cgcctttatg cacgccaata
    51061 tcggcccgtc tgattcgccc attacccgcg cgatcttacg tgcagacgca gagttgaagc
    51121 aggtttcacc gaacctgacc tttatctacg atcctgaaat cacccctgat gacctgctgc
    51181 tggaagtggc gaagaacatc tgtgaatgta gcaaaccgca catcgccaac ggtccggtgc
    51241 atgataaaat tttcacaaaa gggggctacg ggattgtgag ctgttacaac tcactgccgc
    51301 tggcgggtgg tggcagcacg ctggtacgcc ttaacctgaa agccattgcc gagcgcagcg
    51361 aatcgctgga tgacttcttt acgcgcactc taccgcacta ctgccagcag cagatcgcca
    51421 tcatcgatgc gcggtgtgaa ttcctctatc aacaatcaca cttctttgag aatagcttcc
    51481 tggtgaaaga agggctgatt aaccctgaac gttttgtgcc aatgtttggc atgtatgggc
    51541 tggcggaagc ggttaacttg ctgtgtgaaa aagaagggat tgccgcgcgc tacggtaaag
    51601 aagccgccgc aaatgaagtg ggttatcgca tcagcgcgca actggcggag tttgtcgcca
    51661 atacccccgt gaaatatggc tggcaaaaac gcgccatgtt acacgcacag tcggggatca
    51721 gttccgatat cggcaccacg ccgggcgcgc gtttgccgta tggcgatgag ccagatccga
    51781 tcacccatct gcaaactgtc gccccgcatc atgcttatta ttattccggc atcagcgaca
    51841 ttctgacgct cgacgaaacc atcaaacgta acccgcaggc actggtacag ctttgcctcg
    51901 gtgcctttaa agccggaatg cgtgaattta ccgccaatgt cagcggtaac gatctggttc
    51961 gcgttaccgg ttatatggtg cgtttgtcgg atttagaaaa atatcgcgcc gaaggttcac
    52021 gcaccaacac cacctggctg ggcgaagaag ccgcacgcaa cactcgtatt ctggaacgcc
    52081 agccgcgcgt gataagccat gaacagcaga tgcgctttag tcagtaagat tatccccttc
    52141 tcctgcgttg acgggccagg cagtcgtctg gctctgtttt tgcagggctg caatctgcgc
    52201 tgcaaaaact gtcacaatcc gtggacgatg ggacgttgca atgactgtgg ggagtgcgtg
    52261 ccacagtgtc cgcatcaggc gttgcagatt gttgacggca aagtggtgtg gaacgctgtg
    52321 gtctgcgagc aatgtgatac ctgcctgaag aggtgtccgc aacatgccac gccaatggcg
    52381 caatccatga gcgtggatga ggtgcttagc catgtccgca aagcagtgct gtttatcgaa
    52441 gggataacgg tgagtggcgg cgaagccacg acccaactgc cgtttgtagt ggcgctgttt
    52501 actgctatca aaaacgatcc gcaactgcgc cacctcacct gcctggtgga cagtaacggt
    52561 atgttgagcg aaaccggctg ggaaaaattg ctcccggtgt gcgacggcgc aatgctcgat
    52621 ctcaaagcgt gggggagcga atgtcatcag caattaaccg gacgcgataa tcagcagatt
    52681 aagcgcagca tctgtttgct ggcagagcgc ggtaagctgg cggaactgcg tttgctggtg
    52741 atccctagcc aggtggatta tttgcaacac atcgaagaac tggcggcgtt tatcaaggga
    52801 cttggcgatg ttccggttcg cctgaacgcg tttcatgccc acggcgtgta tggcgaggcg
    52861 caaagctggc cgagcgccac gccggaagac gttgagccgt tggctgatgc gttacgggag
    52921 cgcggggtga gccggttaat atttccggcg ctgtatttgt gacatgccgg atacggccta
    52981 cggttcggca cagacttgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcattgtg
    53041 cgccaactgc cggatgcggc gtgaacgcct tatccggcct acggttcggc acaaacctgt
    53101 aggcatgata agacgcgtta agcgtcgcat caggcattat gcgccgactg ccggatgctg
    53161 cctacgaagg gcaaactacc cacgaacgtt aaacaccgca tacgtgttat taagcaacac
    53221 ttcggcaatc tcatcttctg gttccgggcg caactcgcaa agcacgtcaa acacccgcgc
    53281 agcctgttcc gggcggttag gctgcccctg aaagccgttg agcggcatat ccggcgcgtc
    53341 ggtttccagc aacaacgatg ccagcggtaa ttttgcgatg acatcgcggg ttttactggc
    53401 gcgtggatag gtgatagtac cgcctacgcc gattttgtag cccagctgta caaaccgttc
    53461 agcctgttgc tggctgccag aaaatccgtg gacaacacca gcacgcggca aatcgtggcg
    53521 tttaagatgc atcgccagtt tgtcgtgtgt gcgccgtgaa tgcaggatca ccggcagatc
    53581 gtagcgtttc gccagtttca gttgttcgtc gagtaaccac tgctgcctct caaattgcgg
    53641 atcgtcgccg aagagatcca gaccgatctc ccccaccgcc accaccttcg ccggacgcct
    53701 ttccagcgcc tgctgtagct gctcaagaga cacatcgcta tgtttttcca acataccagg
    53761 atgcaagccc aatgcggcat acagcggttg ataattttcc gctaacgcct gcacgcgggc
    53821 aaaattttcc gcctcggttg ccggaacaat gatcttgcct acgcccgctt gtgccgcgcg
    53881 ttgcaggctg gcctcttcat cgccaataaa aggcgggaaa tcaaaatggc agtgggtgtc
    53941 gataaaacgg caaatcaagc cagatcctca ttgttaaatg tcgggtcgtt cgcctgcgga
    54001 gcatcgctca cttcggcaac cagcgtatcg ttggcgactg gcgcaggcgg aataacgatc
    54061 gattccggcg tcaccactgg caccagatgg cgggtaagcg gcgctttttc agttaatagc
    54121 ttgccaaccg tggcgaggaa ataacgaccg cataaacgcc caagtttata gtcttcacgc
    54181 agcgccggaa tccgactgcc aagcgcgata ctatgtaatg gcttcggcgg ataaatttcg
    54241 aatatccgca gcttgcccgg tggtttctca ataaattgct gaatgtcacg atagctggtt
    54301 tcatgatgct gcaccagatt gaccagcggc tgcaggctac tgtcacccag ccagcgttcc
    54361 atgcgtttga accactgcgg cgtgtagtac atttgtgacg gcacagtgcg gatgacgact
    54421 aacgttttag cgccctgcct tgccgcctct ttaaccggaa tcgcatcact gatcccgcca
    54481 tccaggtagt taatgccttc cagcgacact ccgctacgat aaaagccagg tatcgcactg
    54541 gaggcgcgaa tcacatccag ccagttttgt ttggttggta aaaagtaatt cggcgcgtag
    54601 tcatcctgac gacaggcgca catataaaac gatttgccgc tgtcaaacaa ccgcgcggcg
    54661 gtatccattt gcaacggcat ctggcttgca gtggcctcca ccagccagtc gagatcgata
    54721 agatttcctc cacggacaaa gcgcaatgga tcgaaaaatt cgcgttttgt ggtatagcgc
    54781 atgatgactt tgcgcgcgta accgggctga ttgcagataa acgccgagag gttctgcgcc
    54841 ccggcagatg tgccgagata aagatcgaaa ggattaaact gcgcgcgcat aaactcatcc
    54901 agcacgccag ccgtgaaaat tccacgctgc ccgccgcctt cgcacaccag agctattcgt
    54961 ccaggctgga acggccttag cgacaacggc gcaatattac caagcgttac aggtattcgc
    55021 tgccccacct ctgccttcct gtttttgttg tgaaagaatg acaaagtaac gcaatttatt
    55081 attcgctaaa accgcaaaag ccagtccgct ggactggctt tggataaaat tttcttaatg
    55141 gtctgaaatc tagggtcgtt ttcggcccat gaacaaactc accaggaaca gaataatccc
    55201 gacgacaaag acaattttag ctgcgcctgc agcggtaccg gccagaccac caaacccaag
    55261 tgcggcggcg attaacgcga taaccagaaa tatgatgccc caacgaaaca taagcgtctc
    55321 ctttaccata gttaatgtca ccgcttgata tgagcgtgaa aatcactcaa tgcgatattt
    55381 ttagtgtggt gcacatcgcg cctcccgaca aagttcggga ggacgaatta cgacgaatta
    55441 cttagttttc agatcatttt taacgctttt cacaccatct accgctttgg cgatactttc
    55501 agcacggtca ctttgtgcct gagaatctac ggtaccggag agctgaacca cgccatctgt
    55561 tgtttcaact ttcactttac gtgaagggac aatgtcatcc gccagcagtt tggctttgat
    55621 ttcactggtg gtggcggtgt cacccgcgta gcctttcaca gagctttctt tagcgtcgcg
    55681 aacgtgaagt ttgtcgctga cagaggtcac gccctcaacg cctttcgcca ctttcaccgc
    55741 ctcttcggcc tgagcctggc tttcaacgaa accgctcagg gtcacgactt tttgatctgt
    55801 tttgacagag atatcggtgc tcttgatgtt gtcatggtcc actagggccg ccttcacttt
    55861 cgcggtgatg gcgctgtcat ccatgaaatt accgacttta ttcatagagc tatcgacttt
    55921 ttgccctgcg ctttcattgg tagtctgcgc gttgttttct gcgtaggcag agccggtcgc
    55981 gacggcagag gtcaacatta cagccagcag agttttcgaa atcttcagtc ttgtcatagt
    56041 catcgattta ttcctgtatg tttgctcgta atttgagcct ggcaacacga ggttgcattg
    56101 ctgaataggg agagacttca ccctctacag aagtcaatgg tcgccatcac aaaagcgatg
    56161 ggtgatgaat aacgaccatt acagcctctg aatcagttat taatatcggt agaatgacaa
    56221 tcgacggctt tagatactga tatctacgca ttgaacggta tttaacgccg tcaaaaatgt
    56281 cattactttg ttaaatatag atcacaattt tgaaaccgct cgggatatca gcgaaaacat
    56341 aagcaaaagt gaatgtttta agaacattcc gtaagcggct aataaggaag ggaaattgac
    56401 agggcgcagc ggctaccgcg ccctggagac aagaattaat gctcgcgggt ctggtggaac
    56461 tgaacgtccg gataacgttc ctgcgccagg cgcaggttaa ccatgctggt agcgatgtaa
    56521 gcgaggttat cgccgccatc aagcgccagt tggctttcgt tcttacgctt gaactcttcg
    56581 aatttcttcg cgtcagcaca ttctacccac cgggcggtag caacgttgac tgattcatat
    56641 actgcttcaa cgttgtattc gctcttcagg cgcgctacca ccacatcaaa ctgcagcaca
    56701 ccaactgcac caacgatcag atcgttgttg gagatcggac ggaacacctg caccgcgccc
    56761 tcttcggaaa gctgtaccag ccctttgagc agctgttttt gcttcagcgg atctttcaga
    56821 cggatacgac ggaacagttc tggtgcgaag ttcggaatac cggtgaactt catcatctca
    56881 ccctgggtaa aggtgtcgcc gatctgaatg gtgccgtggt tgtgcaggcc gaggatatcg
    56941 cccggatacg cttcttcaac gtgcgaacgg tcacccgcca taaaggtcag cgcgtcggag
    57001 atcaccacat ctttcgcagt gcgcacctgg cgcagcttca tgcctttttc atatttaccg
    57061 gacaccacac gcataaacgc cacgcggtcg cggtgtttcg ggtccatgtt ggcctgaatt
    57121 ttaaatacga agccggtaaa tttgtcttcg ctcgcctcta cggtacgggt atcagtctga
    57181 cgcggcatcg gcgcaggcgc ccactccacc aggccatcca gcatatgatc aacgccgaag
    57241 ttacccagcg cagtaccgaa gaataccgga gtgatttcgc ccgcaaggaa cagctctttg
    57301 tcgaactcgt tagacgcgcc tttcaccagt tccagttcgt cacgtagctg ctgtgccaga
    57361 tcttcaccaa ccgcagcgtc gagatccggg ttattcagcc ctttaacaat acggacttcc
    57421 tgaatggtgt ggcctttacc gctctgatag agataggttt cgtctttata aaggtggtaa
    57481 acgcctttaa acagcttgcc gcagccaatt ggccaggtga tcggtgcgca gccaattttc
    57541 agctcgttct caacttcatc gagcagctcc atcgggtcgc ggatatcacg gtcaagtttg
    57601 ttcataaagg tgaggatcgg cgtgtcgcgc agacgggtaa cttccatcag cttgcgggta
    57661 cgatcttcaa caccttttgc ggcgtcgata accatcaggc agcagtccac cgccgtcagg
    57721 gtacgatagg tatcttccga gaagtcttcg tgccccgggg tgtcgagcag gttaaccagg
    57781 caatcgtgat acggaaactg catcacagac gtagtaatgg agatcccacg ctgcttttcc
    57841 atctccatcc agtccgactt agcgtgctgg ttggaaccac ggccttttac tgtaccggcg
    57901 gtctgaatgg cctgtccgaa cagcagcacc ttctcggtga tggtagtctt accggcgtcc
    57961 gggtgagaaa taatggcaaa agtgcggcgc ttcgccacct cttgcaaata aggagacaac
    58021 gtcataattc tttcttcttg agtaagcggc atcgcgccgc gcttgttgaa aacgaaaaat
    58081 tgcggctatt ttacccatca acggggggga ggcaatcagt gtttacacag gagctgctcc
    58141 agttcgtgca acgaagaaac ggtccaggtg ggcgcgatgc cttctggttg ctcgcgatgg
    58201 tgcgcattca gccagcaggt cgcaagcccg gcgttgatgc caccgagaat atcggactcg
    58261 tcagtgtcgc caaccatcag cacgcgtgaa cggtcaggat tgcccgcctg ttccagtgca
    58321 taatcgaaaa ttttcttatt cggtttagca acgccaactt cttcggaaat caccagcaaa
    58381 tcgaagtaat cacgcaggcc cgtgcgttcc agacgcactt gctgcaaggc gctaaaaccg
    58441 ttggtgatga tgccgatttt ggcgttgcca cgaatggcgt taagcagaga aaccgcgccc
    58501 ggcagcggcg tgcagatttc cgccatcgca ttaataaagg cttcgttgag tttacctggc
    58561 tcgacgttca gccgttcggc ccagctctca aaccgcccgt gctgaagctg taatgaagtg
    58621 atcgcgccgt tttgataatc cacccacagt ggcttgttaa cggcctgata gtcctgaaaa
    58681 tcttcagcgg taaaggtgac gctgtaatca agaaacatcc gctgcaggcc ggtgaatgag
    58741 tcaaaggtaa acagcgtttc atcggcatca aagaaaatcc agtcccactt cattattcca
    58801 ccttgtctta catactgatt ggcaacgcca tgatgatggc gtcttcgcga ccgtccgtgg
    58861 tggggtagta attgcggcga atcgtcgcct cgttaaagcc taaactttcg tacagggcaa
    58921 tggcggcagc atttgaggca cggacttcca gccatagtgt cgccacgccg cgtttttcca
    58981 gttcgtcgat cagatgttcc agcagcgccc ttcccaatcc ctgacgctga tagtcaggat
    59041 cgaccgcaat attgaacaat gtagcttcat ccagcaccac ttgcgtaatc gcaaacgccg
    59101 ccattttgcc gttttgcgtt aactgaaagt tgagataacg ctcgccctgg ttgctggcaa
    59161 aggttttttc actccacgga aaggcgtggg cgcgttgttc aatgtggtaa gccgctggta
    59221 aatcagtcgt ttcgagggaa gaaatcgtgt tcatatgtgc aaatttgttg ccataacgcg
    59281 gcgcgtgccg ttgggtttgc ccgtaattcg gtgagcgccg gtgatgccac ctgagcgcct
    59341 tccaatgata gcggttcgtc agtacccaac cgccagctgt tgcagcgact gccttgcggc
    59401 agcatcgcga ttttttctgg cgtcagttgc agcacttgat cggggctgac ggttaatgcg
    59461 cgcagaacat cgctcactaa aggatcagtc agggcgggaa gatcgtttgc caccatcacc
    59521 agacggacgt gtgccgggat cgcaatggca atctccccct gcaacgcgcc agggcgacgc
    59581 agcgaccact gggtaatgcc cagttgctgt aactgccagt ctcgtcggga tgtcatagcg
    59641 aatcgctcct gttgtcaggg gcgcaaatat agcaaattcg tcgataccgc gccaacatat
    59701 ggctataatc gccgccagta tcaattgagg agcattccat gtctgcattt accccggcaa
    59761 gtgaagtctt gctgcgtcac agtgatgatt tcgaacaaag ccgtattctg tttgccggag
    59821 acttacagga tgacctgccc gcgcgtttag ataccgcggc cagccgtgct catacccagc
    59881 aattccacca ctggcaggta ttaagccgcc agatggggga taacgcccgt ttcagtctgg
    59941 tcgccacggc ggatgacgtc gcagattgcg atacgctgat ttactactgg ccgaagaaca
    60001 aaccggaagc ccagttccag ttgatgaatt tactttctct gctgccagtg gggacagata
    60061 tttttgtcgt tggcgagaac cgcagcggcg tgcgcagcgc cgagcagatg ctggcagatt
    60121 atgcgccgtt gaataaagtc gacagcgctc gtcgctgtgg cctctatttt ggtcgtctgg
    60181 aaaaacagcc ggtatttgat gccgataaat tctggggcga atacagcgtc gatggcctga
    60241 cggtcaaaac gctgcctggc gtgtttagcc gcgacggtct ggatgtcggt agccagttgc
    60301 tgctctcaac gttaactccg cacacgaaag gtaaagtgct ggatgtcggc tgtggcgcgg
    60361 gcgtgctttc ggttgccttt gcgcgccatt cgccgaaaat tcgtctcact ctgtgcgatg
    60421 tctctgcgcc agcggtagaa gccagccgcg caacacttgc cgccaacggc gttgaaggtg
    60481 aagtctttgc cagcaacgtc ttttccgagg tgaaaggtcg ttttgatatg atcatctcca
    60541 acccgccgtt ccacgatggg atgcaaacca gcctggatgc ggcgcaaacg ctgattcgcg
    60601 gcgcagtgcg tcatcttaat agcggcggcg agctgcgaat tgtagcgaac gccttcctgc
    60661 cttatccgga cgtgctggat gagacatttg gcttccacga agtgatcgcg caaacagggc
    60721 gcttcaaggt gtatcgcgcc attatgaccc gccaggcgaa gaaaggttaa ttatctcgcc
    60781 gaataccgtg tcggatgcgg cgtgaacgcc ttatccgacc tacaaaacct gcacgttagc
    60841 ccttcgtagg ccagataaga cgcgccagcg tcgcatctgg catctgcccc cgcatgccta
    60901 ccaaacctgc acgctagttt cctgatggac atttttccag caattacaca tcaggcgata
    60961 attaactatt gacgaaaagc tgaaaaccac tagaatgcgc ctccgtggta gcaattcttt
    61021 ttaagaattg atggtatgcg aaggtggcgg aattggtaga cgcgctagct tcaggtgtta
    61081 gtgtccttac ggacgtgggg gttcaagtcc cccccctcgc accaattatc tttacttcct
    61141 ttcttgtttc ttccctgttt tatcatccct gattcaaatt cattatcatc agcatgatgc
    61201 cagcaataag cgccaggcac gatggtagca aaacgtaatg ccgcaactgt ttgtgataca
    61261 gcatcatcgg cgtcaatact aatcccatca gaatgataat ccgccattga tccggcgata
    61321 aatccgcata catcagccct gcaatcagca atcccggtag caacactccc cagccactgc
    61381 ccagcgtacg ttgcaacatg gtttcatctc tttcattgat aatgataacc aatatcatat
    61441 gataattttt atcatttgca agccagagaa atcccttgct atcgggtaaa cctatcgcta
    61501 ggattagcaa tcattatcat ttagattact atcccgatta tggcctatcg ttccgcaccg
    61561 ctctatgaag atgttatctg gcgaacacat ctccagccgc aggatgctgg gcttgcacaa
    61621 gccgtacggg cgatgatcgc caaacatcgt gaacatttgc tggagtttat ccgcctggat
    61681 gaacccgctc cgcttaacgc catgacgctg gcgcaatggt catcaccgaa tgcgctaagt
    61741 tctctactcg cggtctattc cgatcatatc taccgcaacc aaccaacgat gatccgcgag
    61801 aacaaaccgc tgatctcact gtgggcgcag tggtatatcg gcctgatggt gccaccatta
    61861 atgctggcgc tactgacgca ggaaaaggca ttagatgtgt cgccggaaca tttccatgct
    61921 gagtttcacg aaaccggacg cgtcgcctgt ttctgggtcg atgtgtgtga agataaaaac
    61981 gcaacaccac attcgccgca gcatcgaatg gaaacgttaa tcagccaggc gctggttccg
    62041 gttgtgcaag cactagaagc gaccggagag atcaacggca aacttatctg gagtaatacc
    62101 ggttatttga tcaactggta tctcactgag atgaagcaac tgctcggcga ggctactgtt
    62161 gaatcgctgc gccatgccct cttttttgag aaaacgctca cgaacggtga agataatcca
    62221 ctctggcgta ccgtggtact gcgcgacggc ctgctggtgc gccgcacttg ctgccagcgt
    62281 tatcgcttac cggatttgca gcaatgtggc gattgtacgc tgaaatgaaa gcactgccgg
    62341 ggaagtaaac ccggcatcat gcggattaat agggatgcaa cacattactt gtttcgcaat
    62401 gccgcataca taacagaata tctgcggcct ccaacaaccc tgcgtcagaa ctgacgccca
    62461 gtttcgacat cacattaaat ttatgtgcac ggatcgtttt gatattgcgt ttaagctgct
    62521 cggcaatttg tgtcattgag tagccacgcg acataaagcg caatatttct ctctccgtcg
    62581 ggcttaacgt ccggctttgg ttaatgtacc actgattgtt cagtcggtcg gtcgcctgac
    62641 gtacaccatt taatgacaaa aagagttcct gatgaaaaat ctccagcgtt gacgctttac
    62701 ttaatacacc gtccagcggt gatggcgaca atgaaccaat cagccgagct tcaatatcat
    62761 catccgcaat aactaaacgc cgggtacgcg gaaacttaat cgccagttca gtcaggcaag
    62821 ataatccctc tctgcgctca cttctcatgg cagaaaaaga aaaaatgacc gccgcaaacg
    62881 agatccgcga catggcatcc tgaaatgact cctgactggt ataggtatgg agcttataag
    62941 ggttgcccgc aaactttctg aataaactct caataccgat actactcatg acgcattttt
    63001 ctatgagtgc gacatttctc ttcttaattc ggctgtgttc cattctgcgg catctccata
    63061 aacagaaggc aagttaagtt ctcttgcacc ctgcgcgcta tttatccatg catacatttc
    63121 agcgttgctg tgaattgcca gtcgacgcat cgcactgttt ttttgggcgc tgatagtttt
    63181 attacttttc ttaagcaatg aagctatctg gttgatgccc catcctttac ctaatagccg
    63241 cagtaccttg cgctctgaaa gagtgagaat gacggagcga tagctaaaat catgagtttc
    63301 agtcattgcc ggggaggtca gcatggcgct gatacgctct gcgtgcgtat tgccggaacg
    63361 tacggtcttg accagattct caatgggttc aacatcagac aataacgtcg ccgtagggca
    63421 catgagcaat tctactgcct gggaatacca ggagcgtgac accatgaaaa cccagtgaat
    63481 ttcccgatat tgtgagatta aagaataata atgttcacaa acagaacgtg gatcttcact
    63541 ttcaccggct aaatcagcaa tgactaatcc ggaacgacgt aattgtaata aggtaaggtc
    63601 ctccgcagag gcgctggata ttatttcata ttcagggaag tgagtcctca tgacctcttt
    63661 taaccctgct tgcataacag gtattttact gataacaatt ccatttttgc agcatcctgg
    63721 caacatattt cctccacatc cactgtctaa aaattatgat ggtttattca ggatattcat
    63781 tccttaaatg tttccagcaa attaatagat taagcgaata aattatcctt agcattcact
    63841 ttcatatcag catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat
    63901 caaatgataa cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag
    63961 aaatacttaa tgaaaaacag aaatctcatt catttaattt ataattaaat gatatctctt
    64021 cccgcagaat gcgggaatca cacaatcaat atttattcaa taaaatctga tgattttaac
    64081 ttgttaacac taaaaaacgc gcccagctat ctattatcct gacccgtaaa ggctaaaaca
    64141 ataaattcat tatagtgata atattcatgt gatagtttgt ttgcggcgag agataattcg
    64201 ctttttatca ccgagcttgt cccgataacc aggattaatc agcgcaattt tgatcttaac
    64261 ctaccgcaaa acaaccttgc ttcatgatac aaatggattc tcattatctg aacaatacgc
    64321 agcacgtcta tgacaaaggg cgagttatgc aaactgagca acagcgagcc gtaacacggc
    64381 tttgtatcca gtgtggatta tttcttttgc aacatggtgc ggaaagcgcg ttggttgatg
    64441 agctttcctc acgactgggt cgggcgctgg gaatggacag cgtcgaaagt tctatctctt
    64501 cgaacgccat agtgctgaca actattaaag atgggcaatg cctgacatcg acacgtaaaa
    64561 atcacgatcg cggcattaat atgcatgtgg tgactgaagt ccagcacatt gtgattcttg
    64621 cggagcatca tctgctggat tacaaaggcg tagagaaacg atttagccaa attcagccat
    64681 tacgttaccc aagatggctg gtagccttaa tggttggcct ttcttgcgcc tgtttctgta
    64741 aactcaataa cggtggctgg gatggtgccg tcatcacctt ctttgccagt acgaccgcga
    64801 tgtatatccg ccagctgctg gcacaacgtc atcttcatcc acagatcaac ttttgcctta
    64861 ccgctttcgc cgccaccacc atttccggat tgcttttgca actccccact ttcagcaata
    64921 cccccaccat tgcgatggcc gccagcgttc tgctgctagt gccgggcttt ccgttgatta
    64981 atgccgtcgc cgatatgttt aaaggccaca tcaataccgg actggcacgc tgggcgatcg
    65041 ccagtctgct gacactggct acctgcgtcg gcgtagtgat ggcactgacg atttgggggc
    65101 tacgcggatg ggtgtgatcg aatttctgtt agcgttggcg caggatatga tcctcgccgc
    65161 cattcctgcg gtcggctttg cgatggtgtt caacgttccc gtgcgggcgt tacgctggtg
    65221 tgcgctgctt ggctcgatag gtcatggttc ccgaatgatc ttgatgacca gcgggttgaa
    65281 tattgagtgg tcaaccttta tggcttctat gctggtcggc accattggta ttcaatggtc
    65341 gcgctggtat ctggcgcatc cgaaagtgtt taccgtggcg gccgttatcc ctatgttccc
    65401 gggcatatcg gcttataccg caatgatttc ggcggtaaaa atcagccagt taggttacag
    65461 cgaaccgttg atgattaccc tgttaaccaa ctttcttaca gcttcatcga ttgttggtgc
    65521 gttatccatc ggtctttcca ttcctggatt atggttgtac cgcaagcgcc ctcgcgtata
    65581 aaattgcctc gcttaagcac acggatgaga gacagcctcc tctcctccgt gtgttactat
    65641 aaaagttatc tcccttctcg ttcatcgttc catatttgag aaacagtatg tcttccagag
    65701 ttttgacccc ggacgtcgtt ggtattgacg ccctggtaca cgatcaccaa accgttctgg
    65761 caaaagctga aggcggtgtg gttgccgtat ttgctaacaa tgccccggcg ttttatgccg
    65821 tcacgcctgc acgcctggct gaactgctgg cgctggaaga aaagctggcg cgtccgggaa
    65881 gcgatgtcgc tctggacgat caactctatc aggaaccgca agccgctccc gttgctgtac
    65941 ccatggggaa attcgccatg tatccggact ggcaacccga tgccgacttt atccgcctgg
    66001 cggctctatg gggcgtggca ctaagagagc cggtgaccac cgaagaactg gcctcattca
    66061 ttgcctactg gcaggcggaa ggtaaagtct ttcaccatgt gcagtggcaa caaaaactgg
    66121 cgcgcagcct gcaaatcggt cgtgccagca acggcggact gccgaaacga gatgtgaata
    66181 cggtcagtga acctgacagc caaattccac caggattcag agggtaacga tgaaaaacgt
    66241 tggcgacctg atgcaacgcc tgcaaaaaat gatgcctgcc catatcaaac ctgcattcaa
    66301 aacgggtgaa gagctactgg cctggcagaa agaacaaggg gcgatccgct ccgccgctct
    66361 cgaacgtgaa aatcgggcga tgaaaatgca gcgcaccttt aaccgctccg gtattcgtcc
    66421 actgcatcag aactgctcct ttgagaacta tcgcgttgag tgtgaagggc agatgaatgc
    66481 gttaagcaaa gcgcgccagt atgtcgaaga gtttgacggc aacatcgcca gcttcatctt
    66541 ttctggtaag cctggaaccg gcaaaaacca tctggcggcg gcaatctgca acgagctgct
    66601 gctacgcggt aaatccgtat tgatcatcac cgtggccgat attatgtcgg cgatgaaaga
    66661 taccttcagg aatagcggta ccagcgaaga acaactgctt aacgatctga gcaacgttga
    66721 tctgctggtg atcgatgaga tcggcgtgca gaccgaatcg aaatacgaaa aagtgatcat
    66781 caaccagatc gtcgatcgcc gctcttcttc caaacgccca accgggatgc tgaccaacag
    66841 taatatggaa gagatgacca agttgctggg cgaacgcgtg atggaccgta tgcgcctggg
    66901 taacagtttg tgggtgatct tcaactggga tagctaccgt agccgtgtaa caggtaaaga
    66961 gtattaatcc ccgtttctgg cggtatactg cgacacaatt ccaccttgag taatgatgat
    67021 gaaaactgtt aaacatcttc tgtgctgtgc cattgccgcc agcgcattaa tttccaccgg
    67081 ggtgcatgct gcgtcctgga aagatgcgct ctccagcgcc gccagcgaac ttggcaacca
    67141 aaacagcacg acacaggaag gcggttggtc gctcgcgtca ttaactaact tgcttagcag
    67201 cggaaaccag gccttaagcg cagataacat gaacaatgcc gcaggcattc tgcaatactg
    67261 cgcgaagcaa aagctggcgt cggtaaccga tgccgaaaac atcaagaatc aggtgctgga
    67321 aaagctgggc ctgaacagtg aagagcaaaa agaagacacc aactatctgg acggtattca
    67381 gggtttgctg aaaaccaaag atggtcagca actcaatctg gataacatcg gaacgactcc
    67441 gctggcagaa aaggtgaaaa ccaaagcctg cgatctggtg ttaaagcagg ggctgaactt
    67501 catttcctga accgcgaaac gccgcgtttt agctgttacc cggctgacgc ggctctctcc
    67561 tgcccttttc cctgctcctg gacggttttc cccctgttac acgggaagcg accgcgatca
    67621 aaaaagtctg tttataaact aattcctaac aacatcgcat ttttttgaca ctagaattgc
    67681 agcaatatgg cgacgccatt acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg
    67741 cccggtttag tgaggatcgc ccgttgtcag aactactctc tttcgccctt tttctcgcct
    67801 ctgtgctgat ttacgcatgg aaagcgggac gtaacacctg gtggtttgca gccacgttaa
    67861 cggtgctggg gctatttgtc gttttaaata tcaccctgtt tgccagcgac tattttactg
    67921 gcgatggtat taacgacgcg gttctctata ccttaaccaa cagcctgacc ggtgctggcg
    67981 tcagcaaata cattttgccg ggtatcggca ttgtgctggg gctgacggcg gtgttcggtg
    68041 cgctgggctg gatcctgcgc cgtcgtcgtc atcatccgca ccattttggt tacagcctgc
    68101 tggcgctctt actggcgctg ggttcggtgg acgccagccc ggcatttcgt cagataacgg
    68161 aactggtgaa atcccagtca cgcgatggcg acccggactt tgcggcttat tataaagagc
    68221 cgtcgaaaac tatccctgac ccgaaactca acctggttta tatctacggc gaaagtctcg
    68281 agcggaccta ttttgataac gaagctttcc cggatctcac gcctgaactg ggcgcgttga
    68341 aaaatgaagg cctggatttc agccacacgc agcagctgcc aggaacggat tacacgattg
    68401 cgggcatggt ggcttctcag tgcggcatac cgctgtttgc cccctttgaa ggcaacgcct
    68461 ccgcctctgt ctccagcttc ttcccgcaga acatctgtct gggagatatc ctgaaaaact
    68521 cgggttatca gaactatttc gtgcagggcg caaatctgcg ttttgccggt aaagatgtgt
    68581 tcctgaaatc gcacggcttc gatcacttat acggctcaga agagctgaaa agcgtggtgg
    68641 ccgacccgca ctatcgcaac gactggggat tctacgacga taccgttctc gatgaagcgt
    68701 ggaaaaagtt tgaagaactt tcccgttctg gtcagcgatt ctcactgttt accctgacag
    68761 tcgataccca tcacccggat ggttttatct ctcgtacctg taaccgcaaa aaatatgatt
    68821 ttgacggtaa accgaatcag tcattcagcg cggtaagttg cagccaggag aatatcgcga
    68881 cgtttatcaa caaaatcaaa gcgtcaccgt ggtttaaaga tactgtcatc gtcgtctctt
    68941 ctgaccattt agcgatgaac aacacggcgt ggaaatacct caataagcag gatcgcaata
    69001 acctgttttt tgtcattcgt ggcgacaagc cgcagcaaga gacgctggca gtgaagcgta
    69061 acacgatgga taacggcgcg acggtgctgg acattctcgg tggcgataac tatctcggac
    69121 ttggtcgtag cagtttatcc gggcagtcga tgtcggaaat cttcctcaat atcaaagaga
    69181 aaacattggc gtggaagcca gatatcatcc gcctgtggaa attccctaaa gagatgaaag
    69241 agttcaccat cgaccagcag aaaaacatga ttgccttctc gggtagccat ttccgtttgc
    69301 cgctgctgtt gcgggtttca gacaaacgcg tggaaccgct gccggaaagc gaatactcag
    69361 caccgctgcg tttccagctg gccgatttcg ctccacgcga caatttcgtc tgggttgacc
    69421 gttgctacaa gatggcacaa ctctgggctc cggaactggc actttccacc gactggtgtg
    69481 tctcgcaggg gcagcttggc ggtcagcaaa ttgttcagca tgttgacaaa acaacatgga
    69541 agagcaagac ggcatttaaa gatacggtca tcgacatggc gcgttacaaa ggcaatgtcg
    69601 atacgctgaa gattgttgat aacgatattc gctacaaagc cgacagtttc atctttaacg
    69661 tcgccggtgc gccagaagag gtgaaacagt ttagcgggat ttcccgtccg gagtcgtggg
    69721 gccgctggtc caacgcgcag ctgggcgatg aagtaaaaat cgagtacaag catccgctgc
    69781 caaagaaatt tgacctggtg attaccgcca aagcatacgg caataacgcc agccgtccta
    69841 ttccggtacg cgtaggcaat gaagaacaaa cccttgtgct gggcaatgaa gtgaccacca
    69901 ccacgctgca tttcgataac ccaaccgatg ccgacacact ggtaattgtg ccgccggaac
    69961 ctgtctcaac caacgaaggg aatatcctcg gacactcgcc gcgtaagctc gggatcggca
    70021 tggtggaaat taaagtggta gaacgtgaag ggtaattcct gactgatgtg taggtcgcat
    70081 agggtgcgac ctacacacct tctccggctt acaacatatt attaagaaac aaaaaaacag
    70141 cgcccaacac ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt
    70201 aattacgcct ttaattaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag
    70261 cgtggcgaac ggatagcgat gggttaacat catgtcagca gtgatttttc cttccgccat
    70321 cagtcgaccg actttagcaa aatcttccgg cgtggcgttg cggctaccca tcatcgtcgt
    70381 ttctttttta tggaattccg gatcggagaa ctgcaactca cctttaaaca gaccgacaaa
    70441 taccaccgtg ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat gttgattacc
    70501 tgtcgcgtcg atcactttct gcgccagcga accaccaaac tgcgcccgca gctgcgcgtc
    70561 aaaatcttcg gctgacgggt ccagcacggg caactccaaa cgcgttgcca catgttcacg
    70621 gcgcgccgga ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc
    70681 cgcgcccaga ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc
    70741 tgcgcgacgc accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg
    70801 gtcaataccg tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc
    70861 gccatcctga tgcacgccaa tgactgaaat tttttcacag caattggtac gcccgctttt
    70921 acacgccggg cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt
    70981 aagatcagca atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac
    71041 ccgtggataa ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat
    71101 cccgacagac ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg
    71161 tttccagact aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg
    71221 gctctccatt ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa
    71281 aatctgtttt gtgaaaacac acgcataaaa atgttttaaa tcgggtttta atggactcac
    71341 aaggaggaaa atatgagtcg ttcacaaaat ttacgccaca atgtgattaa ccaggtcatt
    71401 gatgatatgg cgcggggtca tatcccttcc ccacttccat cgcaaagtgc gctggcggaa
    71461 atgtacaaca tcagccgcac tacagtgcgt catattctca gccacttacg cgaatgcggc
    71521 gtcttaacgc aggttggaaa cgactatgtt attgcgcgta aacctgacca tgatgacggt
    71581 ttcgcctgta ctaccgcttc aatgagtgaa caaaacaaag tgtttgagca ggcttttttc
    71641 acgatgataa accagcgtca gttacgccct ggggaaactt tttctgaact gcaactggct
    71701 cgggccgcgg gtgtcagccc ggtggttgta agagaatatc ttttaaaatt cggccgttat
    71761 aatcttattc aaagcgaaaa gcgcggccag tggagcatga agcaattcga tcaatcctac
    71821 gccgagcagc tgtttgagct acgcgaaatg ctcgaaacac attcacttca gcactttctc
    71881 aacctgcctg atcatgatcc ccgctggctg caggcgaaaa ccatgctcga acgtcatcgg
    71941 ctactgcgtg acaacatcgg caatagcttt cgcatgttct cgcaacttga cagagatttt
    72001 cactccttac tgctttcagc tgccgacaat atcttttttg atcaatcgct tgagatcatc
    72061 tcagtcatct ttcattttca ctatcaatgg gacgaaagcg atcttaaaca gcgcaatatc
    72121 attgccgtcg atgagcatat gaccattctc agcgctctga tttgccgcag tgaccttgat
    72181 gccacccttg cgctccgtaa ccatttgaat tcagcgaaac aatcaatgat tcgatccatt
    72241 aatgagaaca cgcgttatgc tcattaagta cggattaaaa acagcaaact attagcgggt
    72301 gcacggtttt atcaaaagat aaataccggg aaattcccgc ttacctatgc tcacaatcag
    72361 tcgactaaaa atgtttttgt gagcggtagt aaagtcctaa aactttaacc tgccgttaat
    72421 gtcaaccttg cgtaatgcac aattacgtaa acaattaaaa ttataaatac aggagtccgg
    72481 cgtggaaaaa gaaaatatca ccctcgatcc gcgttcttca tttactccat cttcgtcggc
    72541 agatattccc gtgccaccag atggattagt tcaacgcagt acccgaatta aacgcattca
    72601 aaccaccgcc atgttgttat tattttttgc ggcggtaatc aattatctcg accgcagttc
    72661 gctgtcggta gcaaatttaa cgattcgtga agaattggga ttaagtgcca ccgaaatcgg
    72721 cgctttgctc tccgtgtttt cactcgctta cggcattgcg caacttcctt gcggcccact
    72781 attggatcgt aaaggcccac gcctgatgct gggactgggg atgttcttct ggtcactgtt
    72841 ccaggccatg tctggcatgg tgcacagctt tacgcagttc gtgttggtgc gtatcggtat
    72901 ggggattggt gaagcgccga tgaacccatg cggtgtaaaa gtcattaacg actggttcaa
    72961 tatcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca ttggcgttgc
    73021 cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg ggatgtttat
    73081 taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct atcgcaaccg
    73141 cgagcacgta gaactgactg ccgttgaaca agcttatctc aatgcaggta gcgtcaatgc
    73201 ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc gcacaatgtg
    73261 gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc tggcctggct
    73321 tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt tgatggcggc
    73381 tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta ctgactggct
    73441 ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg ccgggatgtt
    73501 ctgttctgcc gcctttacgc tgatagtacc acaagcgaca acatccatga tggcggttct
    73561 gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgct ggggcttgat
    73621 ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga actttgccag
    73681 cttcatctgc gcctctttcg cgccgatcat tactggtttt attgttgata caacccactc
    73741 attccgtctg gcactaatca tttgcggttg cgtcaccgca gcgggagcac tggcgtacat
    73801 cttcctggtt cgtcagccga tcaacgaccc gcgtaaagat taaactccct atgcccgata
    73861 acattttgct tatcgggcat ttttatggcg attaaaacgt ttcccagttc tccccgctat
    73921 ctgccactgc cattttacgc ggcgtagctg gcgtcacggt ttttaccaca gccgatgttt
    73981 cacgctgctg ttgctgctgt tgctgaatcc ggaacactgc cacagcttcg gtcaggcgac
    74041 tggcctgctc ttccagcgcg gcggcggcag cggcagactc ttccaccagc gcagcgttct
    74101 gttgagttac ccggtccatc tcagcaaccg ctaagccaac ctgatcgata ccacggctct
    74161 gctcatcaga agcagaagct atttcgccca taatgtccgt cacgcgggtc acagcactga
    74221 caatctccgc cattgtttcc ccggcgcttt cgaccagcgt agagccaaca tccactttcc
    74281 ccaccgagtc ttcaatcagg cttttaattt cacgagccgc ctgggcgcta cgctgggcca
    74341 gattacgcac ttctcccgca accaccgcaa aaccgcgccc ttgctcaccc gcacgcgcag
    74401 cctcaaccgc cgcgttcaaa gccagaatat tggtctggaa agcaatgccg tcaattacgc
    74461 tgataatatc ggcgattttc tgcgaactgg tggagatatc acgcatagtc tgcaccacgt
    74521 tatctaccac tttgccgccg cgttgcgccg tttcagaagc acttaacgcc aggtggctgg
    74581 cctggcgcgc attctcggcg ttctgtttca ccgttgcggt cagttgctcc atgctggctg
    74641 ccgtctcttc cagcgaagcg gcctgttgct cggtgcgcga agagagatca ttattgccgg
    74701 tagcgatttc gctggcaccg ctatagatgg cattggcccc gttgcgcaca tcaccgacgg
    74761 tacgcatcag ttctccctgc atatggcgca aactctctgc cagttgcccc atctcattag
    74821 agccatccac ctcaatcggt ttcaccaaat cgccgcctgc aatatgacga atgctgtcaa
    74881 tcaggcgatt cattggcgct accagcgagg ctttaatacc gaaccagacg gcgaagatga
    74941 ccgccagtac gacgatcatc acgcccacca gaatccacat cgcctggctg taggaggcat
    75001 tgttatcgct gacggcgata tcatagagcc gatcgttttg ctccatgtaa gccacatact
    75061 gcttctcgaa accgtcttga tatccctggg tcggctgatc aaagaactcg ttgattttgc
    75121 ctgcacctaa cagttggatc agctccgcca gcgcattgtg ataaatatcg taattacgtt
    75181 tgatctctgc cgctgcggcg gtgctctgac gcgggtcacg cggcaacgct tcgtaatcca
    75241 cccagttttt ttccgcctgt ttcagcgaaa tactggcact ctgcatcagc tcagcaacgg
    75301 ttgaaccgct accaatatta ttctgatcca tcatgtagcg gatacccgcg cggttgaggg
    75361 tgttacgcgt ctgcaacaac gcgacccagc tgccattcag cgtggattgc tgctggcgaa
    75421 tggtttgtaa aacagtgaaa ttttctttgt cattctttaa ggcattaaag aacagaccgc
    75481 ctgatgtcag ttgtaaaagg ccaaaaacgg ccaaaaccag cagtaagctg gtcacgatct
    75541 tgatacgttt taacatggtt tctctttcct gtggaccgat gcaagatttt cggcctggaa
    75601 agcaaaaact ttatgaaaag gtgtaacccg ccatccatgg aaaaaccagg cgctggttat
    75661 cagatactaa aaagttattc attatcgccc gggatgtctt cttgtcggga attgcccaga
    75721 gcaataaatt cctctaacag atgggttaac tgttgcattt tctcggcagt aaactgagct
    75781 tcaatctgcc gataggcttc ttcaatctgt gtctgggcgc ggttatacag cgcctgcccc
    75841 tctttggtta gcgagatata gagcttccgc tgatcattga ttggctttaa tcgcaacact
    75901 aaaccgtcgc gttccatccg cgtaaggatc ccggtcaggc ttgggcgcaa aatgcaggcg
    75961 cgatacgcca gatcgtgaaa atccattgat gggctttccg ccagaatacg cacgatgcgc
    76021 cactgttgtt cagtcaaatt atgccgttta acgattgggc gaaaataact catcgccgct
    76081 tctcgcgcct ggagcaacgc aatggttagt gagtcgtgca ttatctttcc cctga
//
LOCUS       DAZFFG010000009        69571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFFG010000009 DAZFFG010000000
VERSION     DAZFFG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.009"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            104..325
                     /locus_tag="ACHJ7J_000813"
     CDS             104..325
                     /locus_tag="ACHJ7J_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4999086.1"
                     /translation="MHMNKRTNRQDWHRADIVAELRKRNMSLAELGRSNHLSSSTLKN
                     ALDKRYPKAEKIIADALGMTPQDIWPSRY"
     gene            333..2321
                     /locus_tag="ACHJ7J_000814"
     CDS             333..2321
                     /locus_tag="ACHJ7J_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO4999087.1"
                     /translation="MKEWYTAKELLGLAGLPKQATNITRKAQREGWEFRQVAGTKGVS
                     FEFNIKSFPVALRAEILLQQGRIETSQGYFEIARPTLEAHDYDREVLWSKWDKASDSQ
                     RRLAEKWLPAVQAADEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYGIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGATMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGDRAVDAELFLKTLAEGVAMFNART
                     GRETEMCGGKLSFDDVFEREYARTVVRKPTEEQKRMLLLPAEAVNVSRKGEFTLKVGG
                     SLKGAKNVYYNMALMNAGVKKVVVRFDPQQLHSTVYCYTLDGRFICEAECLAPVAFND
                     AAAGREYRRRQKQLKSATKAAIKAQKQMDALEVAELLPQIAEPAAPESRVVGIFRPSG
                     NTVQVKYQETDDEYETDRDEYLNHSLDILEQNRRKKAI"
     gene            2360..3298
                     /locus_tag="ACHJ7J_000815"
     CDS             2360..3298
                     /locus_tag="ACHJ7J_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4999088.1"
                     /translation="MNISDIRAGLRTLVESEKTTYAQIARESGVAAGTLSAFVNNKYN
                     GDNERVAQTLERWLENYHRAAELPEPPRFVETRTARQIWTSMRFASLTESISVICGNP
                     GVGKTEAAREFRRTNNNVWMITITPSCASVLECLTELAYELGMNDAPRRKGPLSRALR
                     RRLEGTQGLVIIDEADHLGAEVLEELRLLQESARIGLVLMGNHRVYSNMTGGNRTVEF
                     ARLFSRIAKRTAINKTKIDDVKAIADAWQITGENERELLQQIAQKPGALRILNHSLRL
                     AAMTAHGKGERVNEDYLRQAFRELDLDVDISTLLRT"
     gene            3312..3539
                     /locus_tag="ACHJ7J_000816"
     CDS             3312..3539
                     /locus_tag="ACHJ7J_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999089.1"
                     /translation="MMSRNIKMATEVKTWLQARGSHVNESYLGVARPILEITCPPPEL
                     VRSAIRIMEHKSGIARSVWTARFNGCQIIWR"
     gene            3539..3787
                     /locus_tag="ACHJ7J_000817"
     CDS             3539..3787
                     /locus_tag="ACHJ7J_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999090.1"
                     /translation="MMSSEAEKYIKWVKQAQNNNVALCAFNCPKCKEQIMTQCSPENK
                     VWDSFACCPWCNAVYFKQVQGTKVKASVVIIQNSISRQ"
     gene            3790..4059
                     /locus_tag="ACHJ7J_000818"
     CDS             3790..4059
                     /locus_tag="ACHJ7J_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999091.1"
                     /translation="MAKVIFEFNNTENIEFQENGGFAVNMNVRLEESSPEDQTGPHDV
                     MAGIIKSMAPEIIKKATEELLKSARELGLEAEGELFSYNPNAARH"
     gene            4080..4499
                     /locus_tag="ACHJ7J_000819"
     CDS             4080..4499
                     /locus_tag="ACHJ7J_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999092.1"
                     /translation="MNVKIRNEIQALIRIQERNNNGGELREFICARKVDGCDEKTYLI
                     AFDHYSICARYCGESISRAIAFGGAFNVDLWEYVMDREYICASDPEAREMWQRIWRDY
                     RLMAKGWAHCCYSSLALKAVQLSLRHIPASQREPLLY"
     gene            4512..4799
                     /locus_tag="ACHJ7J_000820"
     CDS             4512..4799
                     /locus_tag="ACHJ7J_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999093.1"
                     /translation="MKCHRKRWSREDREFIEANVGKMTVEEMAEKLKVATTALRAHAR
                     RHGISLCVYRISEHDKYLCRELYKEGLDIHVIARKMELSNRAVSSIVYSGY"
     gene            4819..5343
                     /locus_tag="ACHJ7J_000821"
     CDS             4819..5343
                     /locus_tag="ACHJ7J_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4999094.1"
                     /translation="MAKPAKRIKSAAAAYVPQNRDAVITDIKRIGDLQREASRLETEM
                     NDAIAEITEKFAARIAPLKSDIETLSKGVQGWCEANRDELTNGGKVKTANLVTGDVSW
                     RVRPPSVSIRGMDAVMETLERLGLQRFIRTKQEINKEAILNEPGAVAGVAGITVKSGI
                     EDFSIIPFEQEAGI"
     gene            5438..5692
                     /locus_tag="ACHJ7J_000822"
     CDS             5438..5692
                     /locus_tag="ACHJ7J_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999095.1"
                     /translation="MTNQEMKECIVSLCAWHKARVEECRFFIDKKDADITFDNDGEKC
                     VIPAHSKEANLLRLGARLALFIFEKFPVTLNVKDDEDDEE"
     gene            5679..5906
                     /locus_tag="ACHJ7J_000823"
     CDS             5679..5906
                     /locus_tag="ACHJ7J_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999096.1"
                     /translation="MTRNKQQAPEITAEVLIKDSAECLASVPALIQQANAKNLRRAAS
                     FIRAGIARQMMIAQMCMREAQRLEHMEANGD"
     gene            5899..6096
                     /locus_tag="ACHJ7J_000824"
     CDS             5899..6096
                     /locus_tag="ACHJ7J_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999097.1"
                     /translation="MIKSIGFVLLVSTCGNDACDAIPVTERIWPTEQICRQVMEHVQK
                     RYPNEIFYCEEVLRNENTEHY"
     gene            6074..6385
                     /locus_tag="ACHJ7J_000825"
     CDS             6074..6385
                     /locus_tag="ACHJ7J_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999098.1"
                     /translation="MKIQSITDADLMAATLISSGIPEQKVNDFLNNVVYQRYEQARED
                     LISEKRAIIAESLSYAWIIEELRGIIAGQDKLAAVAALDVLQRMSKDLFHTEFIFKEL
                     G"
     gene            complement(6347..6751)
                     /locus_tag="ACHJ7J_000826"
     CDS             complement(6347..6751)
                     /locus_tag="ACHJ7J_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HFO4999099.1"
                     /translation="MKIDYDELARILDVFLDSNDAFITLSDIGINTSHDEQKLIFHLL
                     LLTENGLISNRNLETGSPKCIGLIPTTKGFVWNAIPIRLTQTGHDFANALHKKPILER
                     IKNEFADAPFDVVKDISKSLLAQFFKNKLGME"
     gene            6782..7333
                     /locus_tag="ACHJ7J_000827"
     CDS             6782..7333
                     /locus_tag="ACHJ7J_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO4999100.1"
                     /translation="MSRTSLIKLIHVARRNLQLDDDTYRAFLMQNTGKISCRELTVAQ
                     LELVLDAMKERGFKKQNKHPRRRFKGHVTPREKVLKIWQQMAEDGFIADGSDTALDKY
                     VERLTARRNGGQGVSTLAWCHGESLQIVLETLKQWHIRCIREAFSRYGLPLPVSPSGR
                     ELRGYDAMTAAYARARKTRRLAQ"
     gene            7330..7719
                     /locus_tag="ACHJ7J_000828"
     CDS             7330..7719
                     /locus_tag="ACHJ7J_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO4999101.1"
                     /translation="MTQDLFGDFQDDSILAHLDNESGDTSRFPALLAELNDVLRQELS
                     RLGVEPAHSLEIVVAICKHLGGGQVYIPRGQVLDSLVRDLRIWNDFNGRNVHELAIRY
                     GVTFNTVYKAIRRMRRIKYRQYQPPLF"
     gene            7802..8362
                     /locus_tag="ACHJ7J_000829"
     CDS             7802..8362
                     /locus_tag="ACHJ7J_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFO4999102.1"
                     /translation="MPEFSPAFLHSLNFVIRPDVEGGYVNDPTDRGGETKYGISDRRD
                     GVIDGKTDVNGDGKPDTRIKDLTHEQAAQIYWRDYWLAAKCNEWPDGVSLFVFDAAVQ
                     HGVKRAIQILQAAAGVDDDGIVGKNTRAAVLRADTDWLLLQCFLRRSRYYAGIIKSSA
                     SQGKYLNGWFNRLDLLASVCREVLHG"
     gene            8355..8573
                     /locus_tag="ACHJ7J_000830"
     CDS             8355..8573
                     /locus_tag="ACHJ7J_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999103.1"
                     /translation="MGKGWDASFRFGRRERLRQEVLHREAGGPPPKPMDYTGHDGTHG
                     SYYMKGWQSVDMPEIIHHCQRYREKHHV"
     gene            8566..8946
                     /locus_tag="ACHJ7J_000831"
     CDS             8566..8946
                     /locus_tag="ACHJ7J_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999104.1"
                     /translation="MFKRFCYLLPNCLPVMLLALILIISVFYGLRAVLFWNAAVFSVA
                     LCFTGTIFDDLSRTGKIRRYASINAIDFLGGLVGLSLSAIATGLLEEVHQFTIYALSG
                     FCSIWFCFRLHICLRRSVPSGAEA"
     gene            8943..9068
                     /locus_tag="ACHJ7J_000832"
     CDS             8943..9068
                     /locus_tag="ACHJ7J_000832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999105.1"
                     /translation="MMYRRFSNECSGMATLLMVMKGITVIALLAVVTGICLLVFR"
     gene            9079..9489
                     /locus_tag="ACHJ7J_000833"
     CDS             9079..9489
                     /locus_tag="ACHJ7J_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO4999106.1"
                     /translation="MTNQQDLFEHDPAVSQLMDHIDNIPAPEQEARWPRALVELVDVL
                     ETELKRQGVDDARSIARKQVMSLSWFLGGRQYYIPRGDALLAALRDDLIYCQFNGRNI
                     EELRREHRLSQPQIYKIIARQRKLHSRRHQPDLF"
     gene            9592..10020
                     /locus_tag="ACHJ7J_000834"
     CDS             9592..10020
                     /locus_tag="ACHJ7J_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolysin"
                     /protein_id="HFO4999107.1"
                     /translation="MNDFKKRLPRIIRTLAIVVLAACGGWQLSGDGDSSLVSVQYLPY
                     TDQHGELTVCLNHHGPDIQQRPYSYGECIMLLKADLKPVYDTLNTHLNRPLTEEQKNA
                     LAVFMFSAGSGAVPAPRNNQLLNDDVLPVPGLMPGMSSGL"
     gene            10033..10332
                     /locus_tag="ACHJ7J_000835"
     CDS             10033..10332
                     /locus_tag="ACHJ7J_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO4999108.1"
                     /translation="MDIYSVLTYSIGSGSTIGGILWWAVRRTFASTERVEQIESRLTA
                     LETKCENMPSAQDLHAVRLQTAETNGEVKAMNRHIQVLTHQLELLLEKALGENNK"
     gene            10329..10619
                     /locus_tag="ACHJ7J_000836"
     CDS             10329..10619
                     /locus_tag="ACHJ7J_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO4999109.1"
                     /translation="MIDDILTQDRRLVILRSLMDCNNEANESILQDCLDAYGHNVSRD
                     VVRGQIDWLAEQQLVTVENLRGFYVVTLTSRGQDVAEGRARVSGVKRPRPRF"
     gene            10631..11206
                     /locus_tag="ACHJ7J_000837"
     CDS             10631..11206
                     /locus_tag="ACHJ7J_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HFO4999110.1"
                     /translation="MDRKTRGRASKVDLLPENVRKTLHEMLRDKAIPQARILEEINAL
                     IEDAGLPDDMKLSRSGLNRYATNVEQVGHNLRQMREMTSALTAELGDKPMGETTKLIL
                     EMARSQLFKAMMRQIENPESDVDIDLLKNAMLAAQRLESTAMSSHKREKEIRQAFAEE
                     AANAVSEELRGQDGISEELEQRIRDVLLGKA"
     gene            11220..11414
                     /locus_tag="ACHJ7J_000838"
     CDS             11220..11414
                     /locus_tag="ACHJ7J_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999111.1"
                     /translation="MAVETKEKTSAEIALKLKPIVEAALAEEGNIHIVTKAESLYLLM
                     QIMVGPRIAGQIIRESQINQ"
     gene            complement(11404..11589)
                     /locus_tag="ACHJ7J_000839"
     CDS             complement(11404..11589)
                     /locus_tag="ACHJ7J_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999112.1"
                     /translation="MKEKLTIDQKIEIAKIASDFVIAGMNTNKQPACLAGSDQNVKSE
                     RELLKHIYQLLEAMITD"
     gene            11644..13299
                     /locus_tag="ACHJ7J_000840"
     CDS             11644..13299
                     /locus_tag="ACHJ7J_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO4999113.1"
                     /translation="MSTKESKLKALHAPRKIDLRKEAELLGVNIVTDIGEAQPRNEPV
                     FLGYQRRWFEDESQICIAEKSRRTGLTWAEAGRNVMTAAKPRRRGGRNVFYVGSRQEM
                     ALEYIAACALFARAFNQLAKADVWEQTFWDSDKKEEILTYMIRFPNSGFKVQALSSRP
                     SNLRGLQGDVVIDEAAFHEALDELLKAAFALTMWGARVRIISTHNGVDNLFNQYIQDA
                     REGRKDYSVHRITLDDAIADGLYRRICYVTNQPWSPEAEKAWRDGLYRNAPNKESADE
                     EYGCIPKKSGGAYLSRVLIEAAMTPARDIPVLRFEAPDDFESLTPQMRHGIVQDWCEQ
                     ELLPLLDALSPLNKHVLGEDFARRGDLTVFVPLAITPDLRKRECFRVELRNVTYDQQR
                     QILLFILSRLPRFTGAAFDATGNGGYLAEAARLIYGPEMIDCVNLTASWYQEWMPKLK
                     GEFEAQNITIARHQTTLDDLLHIKVDKGIPQIDKGRTKDEGGKGRRHGDFAVALCMAV
                     RASYMNGFVIDEDSIQALPPRHRGDDVDNDDFDDYHQFERGGW"
     gene            13299..14837
                     /locus_tag="ACHJ7J_000841"
     CDS             13299..14837
                     /locus_tag="ACHJ7J_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFO4999114.1"
                     /translation="MGRILDISGQPFDFDDEMQTRSDELAMVMKRTQEHPSSGVTPNR
                     AAQMLRDAERGDLTAQADLAFDMEEKDTHLFSELSKRRLAIQALEWRIAPARDASAQE
                     KKDADMLNEYLHDAAWFEDALFDAGDAILKGYSMQEIEWGWLGKMRVPVALHHRDPAL
                     FCANPDNLNELRLRDTSYHGLELQPFGWFMHRAKSRTGYVGTNGLVRTLIWPFIFKNY
                     SVRDFAEFLEIYGLPMRVGKYPTGSTNREKATLMQAVMDIGRRAGGIIPMGMTLDFQS
                     AADGQSDPFMAMIGWAEKAISKAILGGTLTTEAGDKGARSLGEVHDEVRREIRNADVG
                     QLARSINRDLIYPLLALNSNSTIDINRLPGIVFDTSEAGDITALSDAIPKLAAGMRIP
                     VSWIQEKLHIPQPVGDEAVFTVQPVVPDNGSQKEAALSADDIQQKDDIDRMSVSPEDW
                     QRSVDPLLKPVILSVLKDGPEAAMNKAASLYPQMDDAELIDMLTRAIFVADIWGRLDA
                     AADH"
     gene            14818..16137
                     /locus_tag="ACHJ7J_000842"
     CDS             14818..16137
                     /locus_tag="ACHJ7J_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016024179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO4999115.1"
                     /translation="MPQQTIDLAYAARLPPKEAVAYFRAKGYNITWNWYEQLADAHAR
                     AFTVAKAARMDVLTTIREEVERAVSEGITREEFTRTLAPRLQKLGWWGKQIIVDAEGN
                     AKEIELGSPRRLATIYNVNTRTAYGAGRYAQMMNTADLYPYWQYVAVMDSRTRPEHAR
                     LHNMIFRYDDIFWQTHYPPNGWSCRCRVRAISATRMKELGLQVSYGASFMDTREVEAG
                     TDESTGEIFRTSSTTFDNGRVKMTPDVGWSYNPGSAAFGTDQALIRKLVEVRDAQLRE
                     QVVQTLNNSRERQLAFSLWLNRLAKSRHAGREIRALGFMTESVAEAVHQRTGNMPARL
                     LVMNGESLATAADAALKAEDLQRLPSLMAEPQAVLWDRKNHQLLYVVATRDGTAQIVV
                     RTSQTVGRQNDRADVLVSISRVSAQSLEAAIADGMIDVLEGHVEVNK"
     gene            16134..16604
                     /locus_tag="ACHJ7J_000843"
     CDS             16134..16604
                     /locus_tag="ACHJ7J_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4999116.1"
                     /translation="MSLDMNVAVDVRRIRLALDDLATVTHDGYVPRVMAAALLSSSEQ
                     AFERQADPDTGQSWESWSDPWLAWRQEHGFVPGSILTLHGDLARSITSAYGPDYALIG
                     SPKVYAAIHQWGGKPDMPPGPAGVPARPYMGLDKSGEQEIYDAIRKRVSAALTQ"
     gene            16806..17891
                     /locus_tag="ACHJ7J_000844"
     CDS             16806..17891
                     /locus_tag="ACHJ7J_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO4999117.1"
                     /translation="MKKHAIGIAALGALSIDDDGWCQLLPAGYFSARDGRPFDVTTGQ
                     GWFIDGEIAGRLIEGIRALNQDVLIDYEHNQLRKDKGLPPEQLVAAGWFNADEMQWRE
                     GEGLFIHPRWTAAAQQRIDDGEFGYLSAVFPYDTATGAVLQIRLAALTNDPGATGMKK
                     LTALAADLPDILQQENKPMNETLRKLLARLGVTVPENADITDEQATAALTALDTLESN
                     AGKVAALSAELEKAQKAAVDLTKYVPVESYNALRDELAQATAQSATASLSAVLDKAEQ
                     EGRIFKSERTYLEQLGGQIGVAALSAQLEKKQPIAALSAMQTTTAKIPSLEKPAVAVL
                     SADEQAAVKALGITEAEYLKMKQEQEK"
     gene            17888..18805
                     /locus_tag="ACHJ7J_000845"
     CDS             17888..18805
                     /locus_tag="ACHJ7J_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO4999118.1"
                     /translation="MIVTPASIKALMTSWRKDFQGGLEDAPSQYSKIAMVVNSSSRTN
                     TYGWLGKFPAMKEWVGKRTIQQMAAHGYSIANKTFEGTVGVSRDDFEDDNLGIYAPMF
                     QEMGRSAAVQPDELIFKLLKDGFTQPCYDGQNFFDKEHPVYPNVDGTGSAVNTSNIVE
                     QDSFSGLPFYLLDCSRAVKPLIFQERRKPELVARTRIDDDHVFMDNEFLFGASARRAA
                     GYGFWQMAVAVKGDLTLDNLWKGWQLMRSFEGDGGKKLGLKPTHIVVPVGLEKSAEQL
                     LNRELFADGNTTVSNEMKGKLQLVVADYL"
     gene            18872..19282
                     /locus_tag="ACHJ7J_000846"
     CDS             18872..19282
                     /locus_tag="ACHJ7J_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001725307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO4999119.1"
                     /translation="MSGTSLNSQRLDTSRITCTAIIKCLRPVYRRAGIAFTRGENTVE
                     VTEEQLAIIRADSVLSVVSTTAAETLAEAGGLDVLGVGDLNARIRATVAGLDQANPEH
                     FTAGGEPKVKAVSAVLGENVTAAQIKAALAEAQA"
     gene            19279..19701
                     /locus_tag="ACHJ7J_000847"
     CDS             19279..19701
                     /locus_tag="ACHJ7J_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp36 family protein"
                     /protein_id="HFO4999120.1"
                     /translation="MSYASLDDMVKRYQRSNLDKLTADKSDNFEPDDALITQALQDAS
                     ALIDGYISPRYQLPLSVVPVTLVRVCCDIAFYYLCDEQATEQCTQRYKDAIRWLGVVL
                     DGKLPLGADVHGVEPVSENLVEVQSDAAIFSRNQKGFI"
     gene            19701..20249
                     /locus_tag="ACHJ7J_000848"
     CDS             19701..20249
                     /locus_tag="ACHJ7J_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp37"
                     /protein_id="HFO4999121.1"
                     /translation="MIATTEAALLDAVRGLFGRTLRDVKTHPGTWSDAAIKKILIAPP
                     SVYLAWLGCGEGRTRREVESRWVFYVVAKMLNGRETDRPGIYQIVERLIAGMNGHTFG
                     PTTGLRLTSVRNLYTDAQSNSGVALYGIYFSGITPLPSGVDLDSLDDYERHWQTWKFP
                     DETPEFAAHINVNQEKDHDAEN"
     gene            20233..20439
                     /locus_tag="ACHJ7J_000849"
     CDS             20233..20439
                     /locus_tag="ACHJ7J_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO4999122.1"
                     /translation="MMLKIKPAAGKAIRDPLTMKLLAPEGEEKPRNSFWIRRLAAGDV
                     VEVGSTENTADDTDAAPKKRSKSK"
     gene            20436..21923
                     /locus_tag="ACHJ7J_000850"
     CDS             20436..21923
                     /locus_tag="ACHJ7J_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4999123.1"
                     /translation="MSDISFNAIPSDVRVPLTYIEFDNSNAVSGTPAPRQRVLMFGQS
                     GSKASAAPNVPVRIRSGSQASAAFGQGSMLALMADAFLNANRVAELWCIPQGNGTGNA
                     AVGEISLSGTAGENGSLVTYIAGQRLAVSVAAGATGAALADLLVARIKGQPDLPVTAE
                     VRADSGDDDTHADVVLSAKFTGALSAVDVRWNYYAGETTPYGIITAFKAASGKNNNPD
                     ISASIAGMGDLQYKYIVMPYTDEPNLNLLRTELQERWGPVNQADGFAVTVLSGTYGDI
                     STFGVSRNDHLISCMGIAGAPEPSYLYAATLCAVASQALSIDPARPLQTLTLPGRMPP
                     AVGDRFTWSERNALLFDGISTFNVNDGGEMQIERMITMYRTNKYGDSDPSYLNVNTIA
                     TLSYLRYSLRTRITQKFPNYKLASDGTRFATGQAVVTPSVIKTELLALFEEWESAGLV
                     EDFDTFKEELYVARNSDDKDRLDVLCGPNLINQFRIFAAQVQFIL"
     gene            21933..22289
                     /locus_tag="ACHJ7J_000851"
     CDS             21933..22289
                     /locus_tag="ACHJ7J_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4999124.1"
                     /translation="MAGNQRQGVAFIRVNGMELETMEGASFTPSGITREEVTGSRVYG
                     WKGKPRAAKLECKIPGGGDIGLDEIIGWENITAEFQADTGETWMMANAWQADEPKNDG
                     GEISLVLMAKQSKRIA"
     gene            22299..22733
                     /locus_tag="ACHJ7J_000852"
     CDS             22299..22733
                     /locus_tag="ACHJ7J_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4999125.1"
                     /translation="MDEMNLSPEAQELRDSIVAELQSGGLKLKDGLPFGTGDETEMQY
                     DVTLRELTAGDLIDAQAAAEKLVMSKEGPVLVSSPSRMGLEMLRRQIASVGCIKGPLS
                     LALMRKLSVDDFQRLSLATEMHDMAVAASLTQERGRVAAVPE"
     gene            22878..24989
                     /locus_tag="ACHJ7J_000853"
     CDS             22878..24989
                     /locus_tag="ACHJ7J_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4999126.1"
                     /translation="MTGKRLKASVIIDLNGNLSRRSRQYSNQINALSRSGQSSLRALR
                     MEVVRVSGAIDRMGSLSTRTFRMLSAGALGVAGVGYTANKLFISAAAQREQQIIAMNS
                     LYHGDKARAQAMMAWAKQNAKDTTWGLSGVLDEIRSSKGFGMTDEQTKQFITMLQDQG
                     AMHGWDLPTAQGASLQLKQMFARQQITAADANLLTGYGINVYQALADATGTDVKKIRD
                     LGTKGKLGMKSILTVFRTLSEQSKGAQASAMNSWDGMFAQMEANLLEFRIKVANSGPF
                     EEIKNEMRRVLNWHDAADKSGELDKRADEIGQKFLTTFRTLKSAAQELWRWLKPGKDA
                     LAWVDQNIVSLKKLAAVLVSVWLANKALRAGWAVAKPSWQVASYPFKTGRRMWRWMRK
                     RKHGQTGIPAPDALPATELLRELTVQRVFVTNWPNGFGGYGGFSRYGGKTPAALPRQP
                     LLLSGPQPLALPAPRTVPALPAPKPLALPAPSPVLALPSPGVPGATRRTSLPLPGKSG
                     LLSRLAGSAAGQLVTGTIGKLAGAGRTVGGLFSGIGNKLADSAIGRVVTKGAGALGWM
                     GKGAGRALSRLGGPVMGALQLAPVLMDEQASTHEKAGAIGSTAGAWLGGAVGSLAGPL
                     GTVAGATLGSVAGEYLGGFVTDLYQKWTAPEREPQEQKVNAEASLRVELGEGLRLTSS
                     RVTEDGMGLNIYAGDNYITGW"
     gene            24994..26481
                     /locus_tag="ACHJ7J_000854"
     CDS             24994..26481
                     /locus_tag="ACHJ7J_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO4999127.1"
                     /translation="MFEDALNAVNSVRDKIGGGKKTTGKGTFRNVPFLVIEEQKQAGG
                     RRLVKREYPLRDTGGVNDLGKKLRSRTFSICILNSNAETARDEAGALMDALDAPGSGE
                     LVHPEFGTVDVMVDSWECRTKADELNYYAFTVTVYPSLQDTAPDAETDTSTAVSAQAV
                     AVTGSLGDTLSSVWQTVKDGTAAATAVMDAVTGVIDDICDAVDNLGITQTISGLMGSL
                     SAMKGSVTGLINKPAMLTSSLMGALSGVSSLCDTRTAFSTWNRLAQRFESRHASTTDR
                     QGAITTSYSSPVAEKNIATLNYVMLAAAQTYRAEAASQALTAALDSSRQMDNAARAPA
                     LDVSVTTTGIASGASSTSSTVTQSQLQLTGITPDGGFSPVSFSDSSTATPPVFESVSD
                     IEKTTAMLGAALDSVILTASEQGFSTDSVQLTQLRLLVVADLEKRGLQLAGSESHHLP
                     ETLPAMVALYRFTGNSRNWQRLARRNGISNPLFVPGGVSIEVINE"
     gene            26474..27613
                     /locus_tag="ACHJ7J_000855"
     CDS             26474..27613
                     /locus_tag="ACHJ7J_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO4999128.1"
                     /translation="MSNTVTLRADGRLFTGWTSVSVTRSIESVAGYFELGVNVPPGTD
                     LSGLAPGKAFTLEIDGQIVCTGYIDSRRRQMTADSMKIIIAGRDKTADLIDCAAVYSG
                     GQWKNRTLEQIARDLCAPYGVTVRWELSDKESSAAFPGFTLDHSETVYEALVRASRAR
                     GVLMTSNAAGELVFSRAASTATDELVLGENLLTLDFEEDFRDRFSEYTVKGYARANGA
                     EGDDIDAKSIVSRKGTATDSDVTRYRPMIIIADSKITAKDAQARALREQRRRLAKSIT
                     FEAEIDGWTRKDGQLWMPNLLVTIDASKYAIKTTELLVSKVTLILNDQDGLKTRLSLA
                     PREGFLVPVESDRKNRKGGDSNGGIDALVEDYYRRHPEKTPPWKE"
     gene            27601..28194
                     /locus_tag="ACHJ7J_000856"
     CDS             27601..28194
                     /locus_tag="ACHJ7J_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4999129.1"
                     /translation="MERVNDSAMNRLLTPLMRRVRLMLARAVVNVINDGRKVQNLQVG
                     LLDDEESDEVERLQNYGHFSVPLPGAEALIACVGAQRDQGIAVVVEDRRYRPTNLEPG
                     DAGIYHHEGHRIRLTKDGRCIITCKTVEVYADEGMTVDTPRTTFTGDVEIQKGLGVKG
                     KSQFDSNITAPDAIINGKSTDKHIHRGDSGGTTGPMQ"
     gene            28191..28628
                     /locus_tag="ACHJ7J_000857"
     CDS             28191..28628
                     /locus_tag="ACHJ7J_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO4999130.1"
                     /translation="MTDLAIIWTNGRGDIAQDGIDMLTDDSLTTDVTISLFTDRRALD
                     SDTLPDGSDNRRGWWGDSYRDRPIGSRLWLLSREKATPDTLERARGYAEEALEWLKTA
                     GRVSAINVRAEQLHQGWLYLYIALTLPDGSVIPYEFKAAFNGV"
     gene            28629..29711
                     /locus_tag="ACHJ7J_000858"
     CDS             28629..29711
                     /locus_tag="ACHJ7J_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4999131.1"
                     /translation="MAYSPPTLSSLIARTEQNIEQRLPGSWPQAREKTLSAIAYAQAG
                     LAAGCHEHISWVGRQIIPSTADEDELLEHCRFWGVRRKQATAASGPLTVTTSAATTIP
                     AGTRWQRADGVVYSLADAIVIDHAGTTEITVTALAAGEAGNTGENTLLTLITPVACVV
                     SDAITVKGFSGGADIESAAELLSRLEYRVQYPPFGGNQFDYVRWAREVSGVTRAWCFP
                     TWKGGGTVGVTFVMDNRSNIFPQPADVERVADYIAGHTDPITGLIVGQPDGVNVTVFA
                     PKAKPVNPRIYISPKTAELKQAITHAINTMFFNEVTPGGALAPSRIIRAVAGVTGLDD
                     FEVRFPTEIQRSENTELLTPGTIEWL"
     gene            29702..30244
                     /locus_tag="ACHJ7J_000859"
     CDS             29702..30244
                     /locus_tag="ACHJ7J_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO4999132.1"
                     /translation="MAVTPYQTAFLQLLPSGLAWNKSPDSKLSALAQAISDVIATAAD
                     DARQMLRERFPSTSRWYLGEWESFLGLPDCTSENGTLSERQRAAANKMRMTGNLSRRF
                     YEWLAAQYGFTVRLTDSTEGQWVTQVNIYGIKNYRNATVLDNVLTPLRVYESGALECL
                     LEKYKPAHQIYKFVYHDGDN"
     gene            30244..31779
                     /locus_tag="ACHJ7J_000860"
     CDS             30244..31779
                     /locus_tag="ACHJ7J_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4999133.1"
                     /translation="MFYIDNDSGVTVMPPVSAQRSAIVRWFSEGDGNNVITWPGMDWF
                     NIVQAELLNTLEEAGIQPDKTKLNQLALSIKAIMNKNALLIKNNLSEIKTAGASAQRT
                     ARENLDIYDASLNKKGLVQLTSATDSSSETLAATAKAVKIAMDNANARLAKDRNGADI
                     PNKPLFIQNLGLQETVNKAANAVQRSGDKMTGELKIGTMNALRIFNDAFGLIFRRSEE
                     SLHFIPTAEGQGENGDIGPLRPFAINLRTGAISVSHGAKIDSGLALGTDNALGGNSIT
                     LGDNDTGIKQGGDGVLLFYSNGQLAFGLQPASADFYKRVAYIHQGITPDGSGAFADQL
                     NNATAPFVQTRFAWNPAPGGHYVPIAKGLSIRNGQGYPGAVSFGYLLTEQMGFPIPCV
                     HMRGDGGNDALWQFNPNDKSFISPGSLVAGGVRYNTDGNIFGGCWGSNLADYLNSTYV
                     RDIRLGSAESISAWRGPGYWDTSGYVLTAAGNSNTDEFIDTLTRRPIQKLIGGTYYNV
                     VSI"
     gene            31781..32308
                     /locus_tag="ACHJ7J_000861"
     CDS             31781..32308
                     /locus_tag="ACHJ7J_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4999134.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLYTEDGKNWYEEQKNF
                     QPDTIKIAYDKNNVIVTVSKDVSTINPEGLSVVEVSDITANRRADNYGNWMFLNGKVV
                     KREYTEQELQQQAELQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(32336..32869)
                     /locus_tag="ACHJ7J_000862"
     CDS             complement(32336..32869)
                     /locus_tag="ACHJ7J_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4999135.1"
                     /translation="MMHLKNITAGNPKTVEQYQLTKQFNIKWLYSDDGRNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWMFKDGVVI
                     KRTYTEEEKRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLFAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(32872..33585)
                     /locus_tag="ACHJ7J_000863"
                     /pseudo
     CDS             complement(32872..33585)
                     /locus_tag="ACHJ7J_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            33651..34244
                     /locus_tag="ACHJ7J_000864"
     CDS             33651..34244
                     /locus_tag="ACHJ7J_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4999136.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIIERTIAGLAAARNKGRIGGRPPKLTKAEWEQAGRLLAQGIP
                     RKQVALIYDVALSTLYKKHPAKRTHIENDDRINQIDR"
     gene            34327..34515
                     /locus_tag="ACHJ7J_000865"
     CDS             34327..34515
                     /locus_tag="ACHJ7J_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4999137.1"
                     /translation="MKSIRCKNCNKLLFKADSFVHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            34466..35161
                     /locus_tag="ACHJ7J_000866"
     CDS             34466..35161
                     /locus_tag="ACHJ7J_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO4999138.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGRAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGVYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            35277..36125
                     /gene="eutH"
                     /locus_tag="ACHJ7J_000867"
                     /pseudo
     CDS             35277..36125
                     /gene="eutH"
                     /locus_tag="ACHJ7J_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
     gene            36122..37525
                     /gene="eutA"
                     /locus_tag="ACHJ7J_000868"
     CDS             36122..37525
                     /gene="eutA"
                     /locus_tag="ACHJ7J_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO4999139.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIEPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            37537..38898
                     /gene="eutB"
                     /locus_tag="ACHJ7J_000869"
     CDS             37537..38898
                     /gene="eutB"
                     /locus_tag="ACHJ7J_000869"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO4999140.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            38919..39806
                     /gene="eutC"
                     /locus_tag="ACHJ7J_000870"
     CDS             38919..39806
                     /gene="eutC"
                     /locus_tag="ACHJ7J_000870"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO4999141.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCTTTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            39816..40475
                     /gene="eutL"
                     /locus_tag="ACHJ7J_000871"
     CDS             39816..40475
                     /gene="eutL"
                     /locus_tag="ACHJ7J_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO4999142.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            40488..40988
                     /gene="eutK"
                     /locus_tag="ACHJ7J_000872"
     CDS             40488..40988
                     /gene="eutK"
                     /locus_tag="ACHJ7J_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO4999143.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            41034..42086
                     /gene="eutR"
                     /locus_tag="ACHJ7J_000873"
     CDS             41034..42086
                     /gene="eutR"
                     /locus_tag="ACHJ7J_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO4999144.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(42092..42991)
                     /gene="hemF"
                     /locus_tag="ACHJ7J_000874"
     CDS             complement(42092..42991)
                     /gene="hemF"
                     /locus_tag="ACHJ7J_000874"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO4999145.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     TRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(42995..43864)
                     /gene="amiA"
                     /locus_tag="ACHJ7J_000875"
     CDS             complement(42995..43864)
                     /gene="amiA"
                     /locus_tag="ACHJ7J_000875"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO4999146.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            44078..44503
                     /gene="ypeA"
                     /locus_tag="ACHJ7J_000876"
     CDS             44078..44503
                     /gene="ypeA"
                     /locus_tag="ACHJ7J_000876"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO4999147.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            44490..44939
                     /gene="yfeZ"
                     /locus_tag="ACHJ7J_000877"
     CDS             44490..44939
                     /gene="yfeZ"
                     /locus_tag="ACHJ7J_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO4999148.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            45146..45721
                     /gene="yfeY"
                     /locus_tag="ACHJ7J_000878"
     CDS             45146..45721
                     /gene="yfeY"
                     /locus_tag="ACHJ7J_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO4999149.1"
                     /translation="MKSLRLLLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            45817..46716
                     /gene="yfeX"
                     /locus_tag="ACHJ7J_000879"
     CDS             45817..46716
                     /gene="yfeX"
                     /locus_tag="ACHJ7J_000879"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO4999150.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDTHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(46774..48078)
                     /gene="pbp4b"
                     /locus_tag="ACHJ7J_000880"
     CDS             complement(46774..48078)
                     /gene="pbp4b"
                     /locus_tag="ACHJ7J_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO4999151.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGINLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(48083..49507)
                     /gene="murP"
                     /locus_tag="ACHJ7J_000881"
     CDS             complement(48083..49507)
                     /gene="murP"
                     /locus_tag="ACHJ7J_000881"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO4999152.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(49511..50407)
                     /gene="murQ"
                     /locus_tag="ACHJ7J_000882"
     CDS             complement(49511..50407)
                     /gene="murQ"
                     /locus_tag="ACHJ7J_000882"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO4999153.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            50571..51428
                     /gene="murR"
                     /locus_tag="ACHJ7J_000883"
     CDS             50571..51428
                     /gene="murR"
                     /locus_tag="ACHJ7J_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO4999154.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            51557..52348
                     /gene="ucpA"
                     /locus_tag="ACHJ7J_000884"
     CDS             51557..52348
                     /gene="ucpA"
                     /locus_tag="ACHJ7J_000884"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO4999155.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            52506..53522
                     /gene="cysP"
                     /locus_tag="ACHJ7J_000885"
     CDS             52506..53522
                     /gene="cysP"
                     /locus_tag="ACHJ7J_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO4999156.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGMGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            53522..54355
                     /gene="cysT"
                     /locus_tag="ACHJ7J_000886"
     CDS             53522..54355
                     /gene="cysT"
                     /locus_tag="ACHJ7J_000886"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO4999157.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            54355..55230
                     /gene="cysW"
                     /locus_tag="ACHJ7J_000887"
     CDS             54355..55230
                     /gene="cysW"
                     /locus_tag="ACHJ7J_000887"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO4999158.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGTGMLVSAFILLVPMVYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            55220..56317
                     /gene="cysA"
                     /locus_tag="ACHJ7J_000888"
     CDS             55220..56317
                     /gene="cysA"
                     /locus_tag="ACHJ7J_000888"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO4999159.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLDHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGRFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDNAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            56451..57362
                     /gene="cysM"
                     /locus_tag="ACHJ7J_000889"
     CDS             56451..57362
                     /gene="cysM"
                     /locus_tag="ACHJ7J_000889"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO4999160.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57365..57733)
                     /gene="yfeK"
                     /locus_tag="ACHJ7J_000890"
     CDS             complement(57365..57733)
                     /gene="yfeK"
                     /locus_tag="ACHJ7J_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO4999161.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            57838..58689
                     /gene="pdxK"
                     /locus_tag="ACHJ7J_000891"
                     /gene_synonym="thiJ"
     CDS             57838..58689
                     /gene="pdxK"
                     /locus_tag="ACHJ7J_000891"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO4999162.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(58732..59241)
                     /gene="crr"
                     /locus_tag="ACHJ7J_000892"
     CDS             complement(58732..59241)
                     /gene="crr"
                     /locus_tag="ACHJ7J_000892"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO4999163.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(59282..61009)
                     /gene="ptsI"
                     /locus_tag="ACHJ7J_000893"
     CDS             complement(59282..61009)
                     /gene="ptsI"
                     /locus_tag="ACHJ7J_000893"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO4999164.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(61054..61311)
                     /gene="ptsH"
                     /locus_tag="ACHJ7J_000894"
     CDS             complement(61054..61311)
                     /gene="ptsH"
                     /locus_tag="ACHJ7J_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO4999165.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(61695..62666)
                     /gene="cysK"
                     /locus_tag="ACHJ7J_000895"
     CDS             complement(61695..62666)
                     /gene="cysK"
                     /locus_tag="ACHJ7J_000895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO4999166.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(62851..63612)
                     /gene="cysZ"
                     /locus_tag="ACHJ7J_000896"
     CDS             complement(62851..63612)
                     /gene="cysZ"
                     /locus_tag="ACHJ7J_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO4999167.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            63842..64840
                     /gene="zipA"
                     /locus_tag="ACHJ7J_000897"
     CDS             63842..64840
                     /gene="zipA"
                     /locus_tag="ACHJ7J_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO4999168.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVLPQVAPSPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            64911..66926
                     /gene="ligA"
                     /locus_tag="ACHJ7J_000898"
     CDS             64911..66926
                     /gene="ligA"
                     /locus_tag="ACHJ7J_000898"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO4999169.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            66928..67146
                     /gene="ypeB"
                     /locus_tag="ACHJ7J_000899"
     CDS             66928..67146
                     /gene="ypeB"
                     /locus_tag="ACHJ7J_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO4999170.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(67143..68141)
                     /gene="yfeH"
                     /locus_tag="ACHJ7J_000900"
     CDS             complement(67143..68141)
                     /gene="yfeH"
                     /locus_tag="ACHJ7J_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO4999171.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            68231..69157
                     /gene="yfeR"
                     /locus_tag="ACHJ7J_000901"
     CDS             68231..69157
                     /gene="yfeR"
                     /locus_tag="ACHJ7J_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4999172.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(69148..69489)
                     /locus_tag="ACHJ7J_000902"
     CDS             complement(69148..69489)
                     /locus_tag="ACHJ7J_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO4999173.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
BASE COUNT        17008 a        17192 c        19123 g        16248 t
ORIGIN      
        1 caaatcacta ttgatttgaa aatgattcca cactacattg cacgatatgc cgtgagctac
       61 tttatagcgt agctcacggg ggaatctgaa aggattacac gaaatgcata tgaataaaag
      121 aactaaccgc caagattggc acagagctga catcgttgca gagctacgaa aacgcaatat
      181 gtcactagct gaattgggaa gatctaatca tctttcgtct tcaacattaa aaaatgcttt
      241 ggataagagg tatccgaaag cggagaaaat cattgcagat gcactgggaa tgacaccaca
      301 agatatttgg ccgtctcgat actaggtgcg ctatgaaaga atggtataca gcaaaagagt
      361 tgctcggttt ggcaggttta ccaaagcaag ccactaacat tacacgtaag gcacaaagag
      421 aaggttggga gttcaggcag gttgcaggaa ctaaaggtgt atcatttgaa ttcaatatca
      481 aatcattccc tgtcgcatta cgtgctgaaa ttctgttgca acaagggaga attgaaacaa
      541 gtcaggggta ttttgaaatc gcccgcccca cgctggaagc ccatgattat gatcgtgagg
      601 tgctgtggag caaatgggat aaagccagcg attcccagcg cagacttgct gaaaaatggt
      661 tgcctgcggt tcaggctgca gacgaaatgc tgaaccaggg gatttcaaca aaaacggctt
      721 ttgcaaccgt tgctggacat taccaggtca gcgcatccac tttgcgggac aagtattacc
      781 aggtacagaa gtttgcgaag cctgactggg cggctgcact tgttgatgga cgtggagcat
      841 cccgtcgcaa tgttcacaaa agtgaatttg acgaggatgc ctggcagttt ctgattgcag
      901 attatctgcg accggaaaaa cccgccttcc gcaaatgtta tgagcgtctg gaactggcag
      961 cccgcgagca tggctggagt attccctccc gtgccacggc ctttcgccgg attcagcaac
     1021 tggacgaggc aatggttgtt gcctgtcgtg aaggtgaaca tgcactgatg catctgatac
     1081 cggcacagca gcgaactgtg gaacacctgg acgccatgca gtggatcaac ggcgacggtt
     1141 atctgcataa cgtctttgta cgctggttta acggtgatgt gatccgtccg aaaacatggt
     1201 tctggcagga tgtgaaaacc cgaaaaattc tgggctggcg ctgcgatgtg agcgagaaca
     1261 ttgattcaat tcgcctctcg ttcatggatg ttgtgactcg ctacggtatc ccggaggatt
     1321 ttcacatcac cattgataac acccgtggtg ctgcgaataa atggctgacg ggaggcgcgc
     1381 caaatcgcta ccgctttaag gtgaaagagg acgatccaaa ggggctgttt ttactgatgg
     1441 gagcgaccat gcactggaca agcgttgttg ccggtaaagg ctggggccag gcaaaacctg
     1501 tagaacgtgc tttcggtgtt ggtggccttg aggaatacgt tgataaacat ccggcactgg
     1561 ctggcgcata tacggggcca aatccgcagg caaaacctga taactatggc gatcgtgctg
     1621 ttgatgcaga gctgtttctg aaaacccttg ccgaaggtgt ggcgatgttc aatgccagaa
     1681 caggtcgtga aacagaaatg tgcgggggta aactctcgtt tgatgatgtt ttcgagcgtg
     1741 aatacgccag aacggttgtg cgtaaaccaa ccgaagaaca aaaacggatg ctgttattgc
     1801 ctgctgaggc ggtgaacgtt tcacgcaaag gcgagtttac gcttaaagtt ggtggctccc
     1861 ttaaaggcgc gaaaaacgtt tattacaaca tggcattaat gaatgccgga gtgaaaaaag
     1921 ttgtggtcag gtttgatcca cagcagctac acagcacggt ttattgctac accctggacg
     1981 gtcggtttat ctgtgaagcg gaatgtctgg caccagttgc atttaatgat gctgcggcag
     2041 gccgtgaata tcgccgccgc cagaaacaac tgaaatctgc aactaaagca gccattaagg
     2101 cgcagaaaca aatggacgcg ctggaagttg ctgaactgct gccgcagata gccgaaccag
     2161 cagcaccaga atcaagggtt gttggtattt tccggccttc cggtaacacg gtgcaggtga
     2221 aataccagga aaccgatgat gaatatgaaa ccgatcgtga tgaatatctg aatcactcgc
     2281 tggatattct ggaacagaac agacgtaaaa aagccattta attaacgttt aaacaaaatt
     2341 taattacgag gttattcaga tgaatatttc tgatattcgc gcaggactgc gcacgcttgt
     2401 agaaagcgaa aaaacaactt atgcgcaaat cgcccgtgaa tctggagttg ccgcaggaac
     2461 gttaagcgcg ttcgtgaata acaagtacaa cggcgataat gagcgtgttg cgcagacgct
     2521 ggaacgctgg ctggagaact atcaccgcgc cgcagaactg ccagaacccc ctcgttttgt
     2581 ggaaaccaga acagcacgtc aaatctggac gagtatgcgc tttgccagcc tgactgaaag
     2641 tatttccgtt atctgcggta atccaggtgt gggaaaaact gaagcagccc gcgaatttcg
     2701 tcgcaccaat aacaatgtct ggatgatcac cattacccca tcctgcgcca gtgttctgga
     2761 atgcctgact gaactggctt atgaactggg aatgaatgat gcaccgcgtc gtaaagggcc
     2821 actctcccgc gctttgagac gccgtctcga aggtacacag gggctggtca ttatcgacga
     2881 agctgatcat cttggcgcag aagttctgga agaactccgc ctgttacagg aatccgcccg
     2941 tattggtctt gttctgatgg ggaatcaccg cgtttattca aatatgactg gcggaaacag
     3001 aacggttgaa tttgcccgcc tgttttcccg tattgcaaaa cggacggcaa tcaataaaac
     3061 caaaattgat gatgtaaagg ccattgcgga tgcctggcaa attaccggcg aaaacgagcg
     3121 ggaattatta cagcagattg cgcaaaagcc aggtgcactt cgtatcctga accactcgct
     3181 gcgccttgct gccatgactg cccacggcaa aggtgaacgt gttaatgaag attatctgcg
     3241 tcaggctttc cgtgaattag acctggatgt tgatatttca accctgctgc gtacgtaagg
     3301 agagagaaat catgatgtca cggaatatca aaatggcaac ggaagtaaag acatggttac
     3361 aggcacgcgg cagccacgtt aatgagtcat atctcggtgt tgcacgcccg attcttgaga
     3421 ttacctgccc accaccggag ctggtcagga gcgccatcag aattatggag cataaatcag
     3481 gaattgcccg ttcagtatgg acagcccgat ttaatggttg tcagattatc tggagataat
     3541 gatgagctct gaagctgaaa aatatattaa atgggttaaa caagcacaga ataacaatgt
     3601 agccctgtgt gcgtttaatt gccctaaatg caaagaacaa atcatgacgc aatgctcacc
     3661 tgaaaataaa gtatgggatt cctttgcatg ttgtccgtgg tgcaatgcgg tttactttaa
     3721 acaggtacaa ggtacgaagg taaaagccag cgtagttatt attcagaatt caataagcag
     3781 gcaataaaaa tggcaaaagt aatctttgag tttaacaata cagaaaatat tgagtttcag
     3841 gaaaatggag gttttgccgt aaatatgaat gtgcggctgg aagaatcctc acctgaggac
     3901 cagaccgggc cacatgacgt aatggcaggc atcatcaaga gcatggctcc tgaaatcatt
     3961 aaaaaagcga ccgaggagtt gttgaaatca gccagggagc ttgggctgga agctgagggg
     4021 gaactattca gctataaccc aaatgcagcc agacattaat tcacaaagga acgctgacaa
     4081 tgaacgtcaa aatccgaaat gaaattcagg cattaatccg aattcaggaa cgcaataaca
     4141 acggtggtga attacgcgag tttatttgcg cccggaaagt tgatggttgt gatgaaaaaa
     4201 cttacctgat tgctttcgac cattacagca tctgtgcgcg ttattgcggc gaaagtatat
     4261 cccgcgccat tgcatttggc ggtgcgttca acgtggattt atgggagtac gtcatggacc
     4321 gggaatacat ctgcgcatca gaccctgaag cccgtgaaat gtggcagcgt atctggcgcg
     4381 attaccggtt aatggcaaaa ggctgggcgc actgctgtta ttcctcgctt gccctgaaag
     4441 cggttcagct atcgctgcgg catattccgg catcgcagcg tgagcctctg ctgtactgaa
     4501 tggtggctga catgaagtgt catcgtaaac gctggtcacg cgaagaccgg gaatttatcg
     4561 aagccaacgt cgggaaaatg accgttgaag aaatggcgga aaaactgaaa gtcgccacaa
     4621 ccgccctccg ggcacatgcc agaaggcacg gaatatcatt gtgtgtatac cgaatcagtg
     4681 aacacgacaa atatttatgc cgtgaacttt ataaagaagg gctggatatt catgtcattg
     4741 cccgaaagat ggaattaagc aatcgagctg tatccagcat tgtatacagc ggatattaat
     4801 taacaggagc tttaatttat ggctaaacca gcaaaacgca tcaaaagtgc cgcagcggct
     4861 tatgtgccac aaaaccgcga tgcggtgatt accgatatta aacgcatcgg ggatttacag
     4921 cgcgaagcat cacgtctgga aacagaaatg aatgatgcca tcgcggaaat tacggagaaa
     4981 tttgcggccc ggattgcacc gcttaaatcc gatattgaaa ccctttcaaa aggcgttcag
     5041 ggatggtgtg aagcgaaccg cgacgaactg acgaacggcg gcaaagtgaa gacggcgaat
     5101 cttgtcaccg gtgatgtatc gtggcgggtc cgtccaccat cagtaagcat tcgtggtatg
     5161 gatgcagtga tggaaacgct ggagcgtctt ggcctgcaac gctttattcg tacgaagcag
     5221 gaaatcaaca aagaagcaat tctgaatgaa ccaggcgcag ttgccggtgt tgccggaatt
     5281 acggttaagt ctggaattga agatttttcc attatcccgt ttgaacagga agccggtatt
     5341 taataccagc attaatattt aattaattaa ctttctttca attatggcgc gatgcgtcag
     5401 gggattgctc gcgcctgaat ctgaaatggt gatatttatg acgaatcagg aaatgaaaga
     5461 atgcattgtc tctttatgtg catggcataa agcacgcgtg gaggaatgtc gtttttttat
     5521 cgataaaaaa gatgcggata ttaccttcga caatgacggt gagaaatgcg ttatccctgc
     5581 acattcaaag gaagcaaacc ttctgagact tggggcaagg cttgcccttt tcatattcga
     5641 aaaatttcct gtaacgctga atgtaaagga tgacgaagat gacgaggaat aagcaacaag
     5701 caccggaaat aacagcggag gtgttgatta aagacagcgc cgaatgccta gcatcagtac
     5761 ctgcattgat tcaacaggcg aatgcaaaaa atctccgtcg tgcagcttcg tttattcgtg
     5821 caggcattgc ccgccagatg atgattgcgc agatgtgtat gcgcgaagca cagcgtctgg
     5881 aacatatgga ggcaaatggt gattaagtca attggctttg tcctgctggt tagcacctgc
     5941 gggaacgatg cctgcgatgc catacccgta acggaaagaa tctggcccac ggagcagata
     6001 tgcaggcagg tgatggaaca cgtgcaaaaa cgttatccca atgagatttt ttattgcgaa
     6061 gaggtattaa gaaatgaaaa tacagagcat tactgatgct gacttgatgg ctgctacatt
     6121 aatttccagt ggcataccag aacaaaaggt gaatgatttt ctgaataatg ttgtgtatca
     6181 acgttatgag caggccagag aagatttaat ttcagaaaag cgcgcgataa tcgctgaatc
     6241 cttaagttat gcatggatta tcgaagagtt gcggggaatc attgccggtc aggataaact
     6301 ggcggcagtt gccgcccttg atgtgctaca acggatgagt aaggatttat tccataccga
     6361 gtttattttt aaagaactgg gctaataaac ttttgctgat gtcttttaca acatcaaatg
     6421 gggcatcagc aaactcattt tttatccgtt caaggatggg tttcttatgt aatgcgttgg
     6481 caaagtcgtg gcctgtctgg gttagtctta tcggtattgc attccagaca aatcccttag
     6541 tggttggtat aagccctata catttaggag atcctgtttc cagatttctg ttactgataa
     6601 gaccattttc tgtcagcaac aataaatgga aaattagttt ctgttcatca tgtgatgtat
     6661 tgatgccaat atcactaagg gtgataaaag catcatttga atccagaaaa acatccagaa
     6721 tccgggccag ttcatcatag tcaattttca tatctcattc cttaatatga gaggtgatca
     6781 aatgagtcgc acatcactga ttaaattaat ccatgtagcc cgtcgtaatc tacaacttga
     6841 cgacgacacc taccgtgcct ttctgatgca gaacacgggc aaaattagtt gccgtgagct
     6901 gaccgtcgcg caactggagc tggtgctcga tgccatgaaa gaacgcggtt ttaagaagca
     6961 gaataaacac ccacgccgtc gctttaaggg gcatgtcacg ccccgcgaaa aggtattaaa
     7021 aatctggcag caaatggcag aagacggttt tattgccgat ggcagtgata cagcactgga
     7081 taaatacgtc gaacgactga cagccagacg taatggcgga cagggtgttt ccacgctggc
     7141 gtggtgtcac ggagaatcgt tacaaattgt gcttgaaacc ctgaaacagt ggcacatccg
     7201 ctgtatccgt gaagcctttt cccgttatgg actcccgttg ccggtcagcc cgtccgggcg
     7261 tgaactgcgt ggctatgatg ccatgacagc agcttatgcc cgtgcaagaa aaacacggag
     7321 actggcacaa tgacgcagga tttgtttggc gactttcagg atgacagtat cctggctcat
     7381 cttgacaatg aatccgggga tacgtcacga tttccggcac tgctggcgga actgaacgat
     7441 gtgctgcgtc aggaactgtc ccggctgggc gttgaacctg cgcactctct ggagattgtt
     7501 gtggctatct gtaaacatct tggcggtggt caggtctata ttcctcgtgg gcaggtgctg
     7561 gattcactgg ttcgggattt acgaatctgg aatgatttta acgggcgcaa tgttcacgaa
     7621 ctggcaattc gttatggtgt gacgtttaac actgtctata aagccatccg acgaatgcgg
     7681 cgtataaagt atcgtcagta tcaaccccct ctgttttaaa cagtaaagcc ggttaatccg
     7741 gcttttttta cgccctcaat attctgttgt gacgataaaa cgttcattac aggagggcaa
     7801 catgcctgag ttttcccctg cattccttca ttcgctgaat tttgttatac ggcctgatgt
     7861 tgaaggtggt tacgttaatg atcccactga ccgtggcggc gaaaccaaat acggcatttc
     7921 tgaccgtcgc gacggcgtta ttgatggcaa aaccgatgtt aacggtgacg ggaagcctga
     7981 tacccgtatt aaagatttaa cccacgagca ggcggcgcaa atctactggc gtgattactg
     8041 gctggcggca aagtgtaacg aatggcctga tggtgtttcc ttattcgtct ttgatgctgc
     8101 cgttcagcac ggtgttaaac gcgcaattca aatcctccag gcggcagcag gtgttgatga
     8161 tgatggcatc gtcggcaaaa acacccgcgc agccgttctg cgtgccgaca ctgactggct
     8221 gctgttgcag tgtttcctgc gtcgttcccg ctattacgca gggattatca aatccagtgc
     8281 ctcgcagggg aaatatctga acggctggtt taaccgcctg gatctgctcg ccagcgtctg
     8341 ccgggaggtt ctgcatgggt aaagggtggg atgcgtcctt tcggtttggc agacgcgaac
     8401 gtttacgaca ggaggtgtta catcgggagg ccggaggtcc accaccaaag ccaatggatt
     8461 acacagggca cgatggcacc cacgggagct attacatgaa aggctggcag tcagtcgata
     8521 tgccagaaat tattcaccac tgccagcgtt acagggaaaa gcatcatgtt taagcgtttc
     8581 tgttatttgt taccaaattg tctgccggtc atgttgctgg cgctgattct gattatcagc
     8641 gtgttttatg gtttgcgggc ggtgctgttc tggaatgctg ccgttttcag tgtggcgctg
     8701 tgctttaccg gcactatttt tgatgatctg tcccggacag gaaaaatccg acgttatgct
     8761 tctattaatg ccattgattt tcttggtggc ctcgtcgggt taagtctttc ggctattgca
     8821 accgggcttt tagaagaagt gcatcagttc acgatttatg ctttatctgg cttctgtagc
     8881 atatggtttt gctttcgcct ccatatctgt ctgcgacgtt cagtaccgtc tggcgcggag
     8941 gcatgatgta cagaagattc agtaatgaat gttccggcat ggctacgttg ctgatggtga
     9001 tgaagggtat cacagttatt gcactgcttg ccgtagtcac ggggatctgt ctgttagttt
     9061 ttcgctgatg ggggcaaaat gacaaatcag caggatttat ttgaacatga tccggctgta
     9121 agccagttaa tggatcatat cgacaatata ccggctcctg agcaggaggc ccgctggccc
     9181 cgcgcgctgg ttgagctggt tgacgttctg gaaacagaac tgaaacgtca gggcgtggat
     9241 gatgcacgga gcatagcccg taaacaggtg atgtccttat catggttcct ggggggacgg
     9301 cagtattaca tcccccgggg tgatgcactt ctggcagcgt tgcgggatga tctgatttac
     9361 tgccagttta acggtcggaa catagaggag ctgcggcgtg aacatcgtct gtcacaaccg
     9421 cagatttaca agattattgc ccggcagaga aaacttcatt cacggcgaca tcagcccgat
     9481 ttattctgag taacagcaat caatcggtgc aggccggttg tgttaattaa taataacccc
     9541 gcctgcaaat tcccctgagt taccctcctt ttattaccga aggagggttt catgaacgat
     9601 tttaaaaaac gtctcccccg catcatcaga acacttgcca tcgtggtact ggcggcatgt
     9661 ggtggctggc agttgtccgg cgatggtgat tcatcgctgg tttctgttca gtaccttcca
     9721 tacacagacc agcacggcga actgacagtt tgccttaacc atcacggtcc tgatattcag
     9781 caacgccctt acagctatgg tgaatgcatc atgctgttga aagcagattt gaagcctgtt
     9841 tatgacacgt taaataccca tctgaacagg ccactgacag aggaacaaaa aaatgcactg
     9901 gcggtgttta tgttcagcgc cggttctggt gctgtgcccg ctccccgtaa taatcagctg
     9961 ctgaatgatg atgtgttacc ggtgccggga ttaatgccgg gtatgtcgtc agggctgtaa
    10021 acaggagagc gtgtggatat ttattcagtg ctgacctact cgataggcag tggcagcacc
    10081 attggtggca ttttgtggtg ggcggtgagg cgtacatttg ccagcacgga acgtgtggag
    10141 caaatcgaaa gccgtttaac cgcgcttgaa acgaagtgcg aaaacatgcc cagtgcacag
    10201 gatttacatg ctgtccggtt acagaccgca gaaaccaatg gtgaagtgaa agcgatgaac
    10261 cggcacatac aggtgctgac ccatcaactg gagcttttgc tcgaaaaggc gctcggagag
    10321 aacaacaaat gattgacgac attctgacgc aggatcgccg cctggtcatc ctgcgttcac
    10381 tgatggactg caataacgag gctaacgaat ccattttgca ggattgcctg gatgcttacg
    10441 ggcataacgt ttcacgtgat gtggttcgtg gtcagattga ctggctggca gaacagcagc
    10501 ttgtcaccgt ggaaaacctg cgcggctttt atgtcgtgac actgacctcc cgtggtcagg
    10561 atgtggctga aggccgggct cgtgtttccg gtgtcaaacg tccacgtccc cgcttctgaa
    10621 cggaggcatg atggacagga aaactcgggg acgggcttca aaagtggatt tgctgccgga
    10681 aaacgtgcgc aaaacactgc atgaaatgct gcgtgacaag gcaatcccac aggcccgcat
    10741 tctggaagaa attaacgccc tgattgaaga tgccggactg cctgatgaca tgaaactctc
    10801 ccgttccggc ctgaaccgtt acgcgacaaa cgtcgaacag gtaggccata acctgcgaca
    10861 gatgcgtgaa atgacgtcag cactgaccgc agaacttggc gacaagccaa tgggggaaac
    10921 cacaaagctg attctggaaa tggcccgcag ccagcttttt aaggccatga tgcggcaaat
    10981 cgaaaacccg gaatcagatg tggatattga cctgcttaaa aacgccatgc tggcagcaca
    11041 gcgtctggaa tccacggcaa tgtccagcca caaacgcgag aaagaaatcc gtcaggcatt
    11101 cgcggaagaa gcggcgaacg ctgtcagtga agaactgcgc gggcaggatg gcatcagcga
    11161 agaactggag cagcgtatcc gtgatgtgct gctgggaaaa gcgtaaagga gaaaatgtaa
    11221 tggctgttga aaccaaagaa aaaacgtctg cggaaattgc cctgaaactc aaacccatcg
    11281 tcgaagcggc gctggctgag gagggcaata ttcatatcgt cacaaaagcc gaatcactgt
    11341 atctgttaat gcagattatg gttggcccgc gtatcgcggg ccagatcatc agagagtcac
    11401 aaattaatca gtaatcattg cctccaacaa ctgataaata tgttttaaca actcccgttc
    11461 tgacttcaca ttttgatcag aaccggcaag acacgctggc tgtttattcg tattcatgcc
    11521 agcgataaca aagtcacttg ctatttttgc aatctcaatt ttttggtcaa tggtcagctt
    11581 ttctttcata gcaacctctt tttactgatt aaagcctgaa aatttatacc ggatttttca
    11641 cctgtgagca ccaaagaaag caaacttaaa gcccttcatg caccccgcaa aatcgacctg
    11701 cgcaaggagg ccgaactgct tggcgtgaat atcgtgaccg atatcgggga agcgcagccc
    11761 cgcaatgaac cggttttcct gggctatcag cgccgctggt ttgaggatga aagccagatt
    11821 tgcattgctg aaaaatcccg tcgtaccggt ctgacatggg ccgaagcggg ccgcaatgtc
    11881 atgacggccg caaagccgcg tcgccgtggt ggtcgcaacg tcttttatgt gggttcccgt
    11941 caggaaatgg cgctggaata catcgccgcc tgtgccctgt ttgcccgcgc ctttaaccag
    12001 ctggcaaagg ctgacgtctg ggagcagacc ttctgggaca gcgacaaaaa agaagaaatc
    12061 ctcacctaca tgatccgctt tccgaatagt ggatttaagg tgcaggcgct ttcaagccgc
    12121 ccgtctaacc tgcgtggttt acagggggat gtggtgattg atgaagcggc atttcatgaa
    12181 gccctggacg aactcctgaa agcggccttt gcgctcacca tgtggggtgc ccgcgtgcgg
    12241 atcatatcga cacataacgg cgtcgataac ctgtttaacc agtacattca ggatgcacgc
    12301 gaggggcgca aggattacag cgttcaccgt atcacgctgg acgatgccat cgctgacggg
    12361 ctgtaccgac gtatctgtta tgtcacgaat cagccgtggt cgccggaagc ggagaaagcc
    12421 tggcgtgatg gcctgtaccg gaacgcgccg aacaaagaga gcgccgacga ggaatacggc
    12481 tgtattccca aaaaatccgg tggcgcttac ctgtcccgtg tcctgattga agctgccatg
    12541 acacccgcgc gggatatccc ggttctgcgt tttgaagccc cggacgattt tgaatcgctg
    12601 acaccgcaga tgcgccacgg tatcgttcag gactggtgtg aacaggagct gctcccgttg
    12661 ctcgatgcgc tgtcgccgct caataaacac gtactgggcg aagactttgc acgccgtggc
    12721 gacctgacgg tgtttgtgcc gcttgccatc actccagact tacgcaaacg cgaatgtttc
    12781 cgcgtggagc tgcgtaatgt gacctatgac cagcagcgac aaatcctttt attcattctg
    12841 tcccgtttac cccgttttac cggcgctgca tttgatgcca ccggtaacgg cggttatctg
    12901 gcagaagccg cccgtctgat ttatggcccg gagatgatcg actgtgtgaa tctgaccgcg
    12961 tcctggtatc aggagtggat gccaaaactg aaaggcgagt ttgaagcgca gaacatcacg
    13021 attgcccggc accagaccac acttgatgac ctgctccaca tcaaggtgga caaaggtatt
    13081 ccgcagattg ataaggggcg cacaaaagat gaaggcggta aaggccgacg acatggcgac
    13141 tttgccgtgg cgctctgtat ggctgtcagg gcgtcttata tgaatggttt tgttatcgac
    13201 gaggacagca tacaggcgct gccaccacgc caccgtggtg atgatgttga taacgatgat
    13261 tttgatgatt atcaccagtt tgaacgaggg ggctggtaat gggccgtatt ctggatattt
    13321 ccgggcagcc gtttgatttt gacgatgaaa tgcagacccg cagcgatgag ctggcaatgg
    13381 tcatgaagcg cacgcaggag catccctcca gtggcgtgac gccgaaccgg gccgcgcaga
    13441 tgctgcgtga tgccgaacgc ggtgatctga cagcacaggc tgacctggcc tttgatatgg
    13501 aagaaaagga cacccacctt ttttccgaac tgagcaaacg ccgccttgcc attcaggcgc
    13561 tggaatggcg catcgctccg gcacgtgatg ccagcgcaca ggagaaaaaa gacgccgaca
    13621 tgctgaatga atatctgcat gatgcggcgt ggtttgaaga tgccctgttt gatgccggtg
    13681 atgccattct caagggttat tccatgcagg agattgaatg gggctggctg ggaaaaatgc
    13741 gcgtgccggt ggcgctgcat catcgcgatc cggcgctgtt ctgcgcgaac cccgacaatc
    13801 tgaacgaatt acgtctgcgt gatacgtctt atcacgggct ggagctgcaa ccgttcggct
    13861 ggtttatgca ccgggccaaa tcccgcaccg ggtatgtcgg caccaatggc cttgtgcgca
    13921 cgcttatctg gccttttatt tttaaaaact attccgtgcg tgattttgcg gagttcctgg
    13981 aaatttatgg actccctatg cgcgtcggta aatatccgac cggctccacg aaccgcgaga
    14041 aagccacgtt gatgcaggcc gtgatggata tcgggcgacg tgccggtggc attattccga
    14101 tggggatgac gctggatttt cagagcgccg cagatggtca gtcagacccc tttatggcga
    14161 tgattggctg ggcagaaaaa gcgatttcaa aagccattct gggcggcacc ctgaccacag
    14221 aggccggaga taaaggtgcg cggtcgctgg gtgaagtgca tgacgaggtt cgccgggaaa
    14281 tccgtaacgc ggatgtgggc cagcttgccc gcagtattaa ccgtgacctg atttatcccc
    14341 tgctggcact gaacagtaac agcaccattg atattaaccg cctgccgggc attgtatttg
    14401 ataccagtga agcgggtgat attacggcac tgtctgacgc cattccgaaa ctggcggcag
    14461 gaatgcgtat tccggtctcc tggatacagg aaaaacttca tattccacag ccggtcggtg
    14521 atgaagccgt gtttactgta caaccggttg tcccggataa cggttcgcag aaagaggccg
    14581 cattatccgc tgatgatatc cagcagaagg atgatatcga ccggatgagc gtttcaccgg
    14641 aagactggca gcgttcggtt gatcccctgc tgaaacccgt cattctttct gtgctgaaag
    14701 atggcccgga agccgccatg aacaaagccg cttcattata tccgcagatg gatgatgcag
    14761 aacttatcga catgctgacc cgtgccattt ttgttgccga tatatggggg cgtctggatg
    14821 ccgcagcaga ccattgatct ggcgtatgcc gcacgcctcc cgccaaagga ggctgtcgcg
    14881 tatttccgcg ccaaaggcta caacatcacc tggaactggt acgaacaact ggcagacgcg
    14941 cacgcccgtg cctttaccgt tgccaaagct gcccggatgg atgtcctgac cacaatccgt
    15001 gaggaagtgg agcgggctgt cagtgagggt atcacccgtg aggaatttac ccgcacactg
    15061 gctccccggt tgcaaaagct cggatggtgg ggaaaacaaa tcattgtgga tgcagaggga
    15121 aacgcgaagg aaatcgaact gggcagtccg cgtcgtctgg caaccattta taacgtcaat
    15181 acccgtacag cgtacggggc gggacgttat gcacagatga tgaataccgc agacctgtat
    15241 ccgtactggc agtatgtcgc cgttatggac agcagaacgc gcccggaaca tgcccggctg
    15301 cataatatga ttttccggta tgacgatatt ttctggcaga cacattaccc gcccaatggc
    15361 tggagctgtc gctgccgcgt tcgtgcgata tctgcgaccc gaatgaaaga actgggatta
    15421 caggtcagct atggtgcttc attcatggat acccgcgaag tggaggccgg cacggatgaa
    15481 agcacaggag agattttccg cacatcgtca accacgtttg acaacggtcg ggtaaaaatg
    15541 acgccggacg ttggctggtc atataacccc ggttcggcgg cattcggtac tgatcaggcg
    15601 ctgattcgaa aactggtgga agttcgtgac gcgcagctgc gtgagcaggt tgtacaaacg
    15661 ctgaataaca gccgggagcg ccagcttgct ttttccctgt ggcttaatcg tctggcgaaa
    15721 tcacgtcacg ccgggcgtga gatccgggca ctgggtttta tgacggaatc ggtagctgaa
    15781 gccgtacatc agcgcaccgg gaatatgcca gcccgtttac tggtgatgaa tggagaaagt
    15841 ctggctaccg ctgctgatgc tgccctgaaa gcagaggatt tacagcgtct gccgtctctg
    15901 atggcagaac cacaggcggt attgtgggac agaaaaaatc accagctgct gtacgttgtg
    15961 gcaacccgtg acggcacggc ccaaattgtg gtcagaacat cacagacagt cggcaggcag
    16021 aatgaccggg cggatgtgct ggtcagtatc agccgcgtat ctgcacagtc gcttgaagcc
    16081 gcgattgctg acgggatgat tgacgtactg gaagggcatg tggaggtgaa taagtgagtc
    16141 ttgatatgaa cgttgccgtg gatgtgcgac gtatccggct tgctcttgat gatctggcaa
    16201 cggtaacgca tgacggatat gtgccacgtg tgatggctgc ggcattgctg tcgtcttctg
    16261 aacaggcttt tgaacggcag gcagacccgg acacgggcca gagctgggag tcatggagtg
    16321 acccgtggct ggcatggcgt caggagcatg gttttgttcc gggtagcatt cttaccctgc
    16381 atggcgatct ggcccgcagc atcacttccg cttacggccc ggattatgcc ctgattggct
    16441 caccaaaagt ttacgctgct attcatcagt ggggcggtaa gcctgatatg ccccccggtc
    16501 cggcaggtgt tcctgcacgt ccttatatgg ggctggataa gagcggtgag caggaaattt
    16561 atgatgccat cagaaaacgc gtaagcgccg ctctgacgca gtaaccagat acagagataa
    16621 aatcacgcga taaagaattt ttgaaggttc tgtggcgttt ttgaaggggt tttgaagggg
    16681 gtagcgtcat gcatttccct tcccctttat ctttctccta cagaattact aaaactggat
    16741 aaccccgcct tttctcccgt tccccgcaca ctggctactc acaaatcacg atgagcaggc
    16801 cggacatgaa aaaacacgcg attggtattg ctgcgctggg tgcgctgtcc attgacgatg
    16861 acggctggtg ccagctattg cctgccggtt atttcagtgc ccgtgacggt cgcccgtttg
    16921 atgtaacaac tggtcagggc tggttcatcg acggagaaat tgccggacgc ctgattgaag
    16981 gtatccgcgc acttaaccag gacgtgctga ttgattacga gcataaccag ctgcgtaagg
    17041 acaaaggatt acccccggaa caactggtcg cggctggctg gtttaacgcc gatgaaatgc
    17101 agtggcgtga aggtgaaggg ttatttatcc acccacgctg gacagcggca gcccagcagc
    17161 gcatcgatga cggtgaattt ggttatctct ctgcggtttt tccgtatgac accgccactg
    17221 gtgcggtact gcaaatccgc cttgctgcgc tgaccaatga tcccggggcc accggcatga
    17281 aaaagctgac cgcactggcg gcagaccttc ccgacatcct tcaacaggag aacaaaccca
    17341 tgaatgaaac gctgcgcaag ctgcttgcgc gtcttggggt gactgtcccc gaaaatgcag
    17401 acatcaccga tgaacaggca acagcggcac tgaccgcgct ggacacgctg gaaagtaacg
    17461 ccgggaaagt ggcggcgttg tctgccgaac tggaaaaggc acagaaagcc gccgttgacc
    17521 tgacgaaata cgtcccggtg gaaagctaca acgccctgcg tgatgaactg gcacaggcga
    17581 ctgcacaaag cgccacagcc agcctgagcg ccgttctgga caaggcggaa caggaaggcc
    17641 gcattttcaa aagcgaacgt acctatctgg aacagcttgg cggtcagatt ggcgtggcgg
    17701 cactgtcggc acagcttgag aaaaaacagc ccatcgctgc gttatctgcc atgcagacca
    17761 ccacagcgaa aatcccgtcg ctggaaaaac cggcggtggc ggtgctgtct gctgatgaac
    17821 aggcagcagt gaaggcgctc ggcattactg aggctgagta tctgaaaatg aaacaggaac
    17881 aagaaaaatg attgtcaccc cggcatccat taaagccctg atgacctcct ggcgtaagga
    17941 ttttcagggc ggtcttgaag acgccccgtc gcagtacagc aaaattgcaa tggtggtgaa
    18001 ctcctcatcc cgcactaata cctatggctg gctgggtaaa ttccctgcca tgaaagaatg
    18061 ggtgggtaag cgaaccattc agcaaatggc agcgcatggc tactccatcg ccaataaaac
    18121 gtttgaaggc accgtgggcg taagccgtga tgacttcgag gatgacaatc tgggtattta
    18181 tgcgccgatg tttcaggaga tgggccgttc tgcggcagtt cagccggatg agctgatttt
    18241 taaacttctg aaagatggct ttacccagcc ctgctatgac ggtcagaact tcttcgacaa
    18301 ggagcatccg gtatatccga atgtggacgg cacgggcagc gcggttaata cctccaacat
    18361 cgttgagcag gacagcttca gcggtctgcc attctatctg ctggactgct cccgcgcagt
    18421 caaaccgctg attttccagg agcgccgcaa accggaactg gtcgcacgta cccgtatcga
    18481 tgacgaccac gtttttatgg ataacgagtt cctgtttggt gccagcgccc gccgtgctgc
    18541 cggttacggc ttctggcaga tggctgtcgc ggtaaaaggc gatctgacgc tggataacct
    18601 ctggaagggc tggcaactga tgcgttcttt tgaaggtgat ggcggtaaga agctgggcct
    18661 gaagccgacg catatcgttg taccggtcgg actggaaaaa tccgcagagc aactgcttaa
    18721 ccgtgagctg ttcgcggatg gcaataccac cgtctctaac gagatgaagg gtaagctgca
    18781 actggttgtc gctgactacc tgtaaccgtc gttaaaaggc ggttcaggcc gccttttaac
    18841 ctgatttaaa ggaaaaggag gagcatgtgt tatgtcaggc acttcgctta attcacaacg
    18901 gctggacact tctcgcatca cttgcacggc gattattaaa tgtcttcgcc cggtctaccg
    18961 ccgcgccggt atcgccttta cgcgtggcga aaacacagtg gaagtcaccg aagaacaact
    19021 ggcgatcatc cgtgccgaca gcgtgttgtc tgttgtatcg acaacggcag cagaaacgct
    19081 cgctgaggct ggggggctgg acgttctggg tgtgggcgat ctgaacgccc gtatccgtgc
    19141 gaccgttgcc ggtctggatc aggcaaaccc ggaacacttt accgcagggg gagaacccaa
    19201 agtgaaagcg gtcagtgcgg tgctggggga aaacgtcact gcggcacaaa tcaaagcagc
    19261 actagctgag gcacaagcat gagctatgcc tctcttgacg acatggtaaa gcgttaccag
    19321 cgcagcaatc tggacaaact gaccgctgat aaatcggaca actttgaacc agatgatgca
    19381 ctgattacgc aggcgttaca ggatgccagc gcgcttatcg acggttatat cagtccccgt
    19441 taccagctgc cattgtcagt ggttcctgta acgctggttc gtgtttgttg cgatattgct
    19501 ttttattacc tgtgtgatga acaggcaaca gagcaatgca cacaacgtta caaagacgcc
    19561 atcaggtggc tgggggtggt tctcgacgga aaattaccgc ttggcgcaga tgtgcatggt
    19621 gttgaaccgg tcagcgaaaa tctggttgag gtgcagtctg acgcagccat tttctcccgc
    19681 aaccagaagg ggtttatctg atgattgcca ccacagaagc tgctttgctc gatgctgttc
    19741 gtgggttgtt cggacggacg ctgcgggatg taaaaacgca tcccggcacc tggtctgatg
    19801 ccgcaattaa aaaaatcctg attgcgccgc cgtctgttta tctggcgtgg ctgggctgcg
    19861 gtgaaggccg cacccgccgt gaagtggaaa gccgctgggt gttttacgtg gtggcaaaaa
    19921 tgctgaacgg tcgggaaacc gaccgtccgg ggatttacca gattgttgag cgcctgattg
    19981 ccggaatgaa cggacataca tttggcccca caaccggcct gcgtctgacc agcgtccgca
    20041 acctgtatac cgatgcccag agtaattcag gagtcgcatt gtacggtatc tatttcagtg
    20101 gcatcacgcc gttaccgtcc ggtgtggatc tggactcgct ggatgattac gagcgccact
    20161 ggcagacctg gaaattcccg gacgaaaccc cggaatttgc cgcacatatc aatgtgaatc
    20221 aggaaaagga tcatgatgct gaaaattaaa cccgcagcgg gaaaagccat ccgtgacccg
    20281 ctcacgatga aattactggc tccggaggga gaagaaaagc cccgtaacag tttctggata
    20341 cgtcgccttg cagccggtga tgtggttgaa gtcgggagca ccgaaaacac ggcggatgat
    20401 accgacgctg cgccgaaaaa acggagtaaa tcgaaatgag cgatatttca tttaacgcga
    20461 tcccgtcaga tgttcgcgtt cccctgacgt atatcgaatt tgataacagt aatgccgtca
    20521 gcggaacacc ggcaccccgt cagcgcgtgc tgatgttcgg gcaaagcgga agtaaagcca
    20581 gtgcggcacc aaacgtgcct gtccgtatcc gttccggctc acaggccagt gcggcgtttg
    20641 gtcagggttc catgctggca ctgatggcag atgcattcct gaacgctaac cgcgtggcgg
    20701 agctgtggtg tatcccgcag gggaacggca ccggtaatgc tgctgtcggt gaaatctcac
    20761 tgtcaggaac ggcaggcgaa aacggctcgc ttgtgactta cattgccggt cagcgactgg
    20821 cggtatctgt cgcagcaggt gcaacgggag cggcgctggc tgacctgctg gttgcccgaa
    20881 tcaaaggcca gcctgattta ccggtgacgg cagaagttcg cgcagacagc ggggatgatg
    20941 acacccatgc agatgtggtt ctgagtgcaa aatttacggg tgcattatct gccgtggatg
    21001 tgcgctggaa ctattacgcg ggtgaaacca ccccttacgg gattatcacg gcattcaaag
    21061 ccgcatccgg gaaaaacaac aacccggata tcagcgccag catcgcgggg atgggtgatc
    21121 tgcaatacaa atatattgtg atgccttaca ccgatgaacc aaacctgaat ctgttacgca
    21181 ctgaattgca ggaacgctgg gggccggtca atcaggctga tggctttgca gtaacggtgt
    21241 tgtccggcac gtatggggac atttccacgt ttggtgtcag tcgtaacgac catctgatct
    21301 cctgtatggg aattgccggt gcaccggaac cgtcatatct gtacgccgcc acactgtgtg
    21361 ccgttgccag tcaggcgctt tccatcgacc cggcacgtcc gctccagacg ctgacgctgc
    21421 ccggaagaat gccgcctgcg gtgggtgatc gtttcacctg gtcagaacgt aatgccctgc
    21481 tgtttgacgg catctccacg tttaacgtga atgatggtgg tgaaatgcag attgaacgca
    21541 tgatcacgat gtaccgcaca aacaagtacg gtgacagcga tccgtcttat ctgaacgtga
    21601 ataccatcgc cacgctgagt tatctacgtt attcgctgcg aacccgcatc acgcagaaat
    21661 tcccgaacta caaactggca agtgatggca cccgctttgc cactggtcag gccgtggtga
    21721 cgccatccgt gatcaaaacg gagctgctgg cactgtttga agagtgggaa agcgccgggc
    21781 tggttgagga tttcgacacg ttcaaagagg agctgtacgt tgcacgtaac agcgatgaca
    21841 aagaccgtct tgatgtgctg tgcggcccga atctgattaa ccagttccgc attttcgcgg
    21901 cacaagttca gttcattctg taaggagcat ttatggctgg aaatcagcgt cagggggtgg
    21961 cgttcatccg tgtcaatggc atggagcttg aaacgatgga gggggcctcc ttcacacctt
    22021 ccggcatcac ccgtgaagaa gtgaccggct cgcgggttta tggctggaaa ggtaaaccac
    22081 gtgccgcaaa gctggaatgc aaaattccgg gcggtggtga tatcggtctg gatgaaatca
    22141 tcggctggga aaatatcacg gcagagtttc aggcagatac cggggaaacc tggatgatgg
    22201 cgaatgcctg gcaggcagat gaaccgaaaa acgacggcgg cgaaatttcg ctggtactga
    22261 tggcaaaaca aagcaaacgc attgcataag gggaaacaat ggacgaaatg aatttaagtc
    22321 ctgaagcaca ggaactgcgt gacagtattg tggcggagct tcagtccggt gggctgaaac
    22381 tgaaagacgg cctgccgttc ggcaccggtg acgagactga aatgcagtat gacgtcacac
    22441 tgcgtgaact gaccgcaggc gatctgattg atgcccaggc ggctgctgaa aagctggtaa
    22501 tgagcaagga aggcccggtg ctggtcagtt ccccgtcccg gatgggactg gaaatgctgc
    22561 gccgacagat tgccagcgtg ggttgcatca aaggcccgtt atcgctggcg ctgatgcgca
    22621 agctgtctgt tgatgacttc cagcgtctgt cgctggctac agagatgcac gacatggctg
    22681 tggcggcatc gctgacgcaa gaacgggggc gagtggctgc ggtgccggaa tgatattgag
    22741 aaagcggcga cagcgattgg cgttattctg aaaagtggcc cggaatgggc cttgtctctc
    22801 ccgctgtccc gctttttccg gcactgccag caggctaaaa ccctctctca atatcaccgt
    22861 taaatcagga aatatctatg accgggaaac gcttaaaagc gtcagtcatt attgacctta
    22921 atggcaatct ttcccggcgt tcccgccagt attcgaacca gataaacgcc ctgtcccgta
    22981 gtgggcaaag ttccctgcgt gccctgcgta tggaagtggt gcgcgtgtct ggtgccatcg
    23041 acagaatggg ttctctctca acccgtacat tccgaatgct gtcagccggg gcgctgggtg
    23101 ttgccggggt gggttacacc gccaataaac tgtttatcag cgcagcagct caacgtgaac
    23161 agcagattat cgccatgaac tcgctgtatc acggcgataa ggccagagcg caggcaatga
    23221 tggcctgggc gaagcagaac gcgaaggaca ccacgtgggg gctgagtggt gttcttgatg
    23281 agatccgttc gtcgaaaggc ttcggcatga cggatgaaca gacgaaacag ttcatcacca
    23341 tgttgcagga tcagggggcc atgcacggct gggatttacc cactgcacag ggtgcctcac
    23401 tgcaactgaa acagatgttc gcccgccagc aaatcacagc ggcggatgcc aacctgctga
    23461 ccggttacgg tatcaacgtt tatcaggcgc tggcggatgc aacgggaacg gacgttaaaa
    23521 aaatccgtga tctggggaca aagggcaagc tgggcatgaa atccattctg acggtattca
    23581 gaacactgtc agaacagtca aaaggtgcac aggccagtgc gatgaactcc tgggacggga
    23641 tgttcgccca gatggaagcc aacctgcttg aatttcgcat aaaggtggca aacagcggcc
    23701 cttttgaaga aatcaagaat gagatgcgcc gggtgcttaa ctggcacgat gcggcggata
    23761 aatccgggga gcttgataaa cgggctgatg aaattggtca gaaattcctg accacattca
    23821 gaaccctgaa atctgcggca caggagttat ggcgctggct gaaaccgggt aaagatgcgc
    23881 tggcatgggt tgaccagaat attgtcagcc tgaaaaaact ggctgctgtt ctggtgtctg
    23941 tctggcttgc caataaagca ctgagggcgg gctgggccgt ggcgaagcca tcatggcagg
    24001 ttgccagcta tccgttcaaa accgggcgtc gaatgtggcg ctggatgcga aaacgcaaac
    24061 atggacagac aggcattccg gcaccggatg cgctacctgc tacagaacta ctgcgggaac
    24121 tgacagttca gcgcgttttt gtgactaact ggcccaacgg gttcggtgga tatggcggat
    24181 tttcccgata cggaggtaaa acaccggcag cattgccccg ccagccgtta ttgctgtccg
    24241 ggccacaacc gctggcactt ccggccccac gcactgttcc cgcattacca gcaccgaaac
    24301 cactggcatt acctgcacca tccccggtac tggcattgcc atctcccggt gtgccggggg
    24361 ccacacggcg cacatcgtta cctttacccg gcaaatccgg tctgttgagc aggcttgcag
    24421 gcagtgctgc cggacaactg gttacgggaa caatcggaaa gctggctggt gccggtcgta
    24481 ccgttggcgg gttgttttcc ggtatcggaa acaaacttgc agacagtgcc attggtcggg
    24541 ttgtgacaaa aggggccggg gcgctgggct ggatggggaa aggcgcgggt cgtgcactgt
    24601 cacgtcttgg cggtccggtg atgggggcac tccagcttgc tcccgttctg atggatgaac
    24661 aggcatcaac tcatgaaaaa gccggagcaa ttggcagcac tgccggcgcc tggctcggcg
    24721 gtgccgttgg cagccttgcc ggaccgctgg gcacggttgc cggtgccacg ctgggcagtg
    24781 tcgccgggga atatctgggc ggttttgtga ctgacctgta tcagaaatgg acggccccgg
    24841 aaagggaacc gcaggaacaa aaagtgaatg cggaagcatc gctgcgtgtc gagcttggcg
    24901 aaggattacg tctgaccagt tcccgcgtca ccgaggatgg tatggggctg aatatttacg
    24961 cgggcgataa ctacattaca ggctggtaaa accatgtttg aagatgcttt aaatgccgtt
    25021 aattctgtcc gggataaaat cggcggtggt aagaaaacaa ccggcaaagg cacgttccgt
    25081 aacgtgccgt ttctcgtcat tgaggagcaa aaacaggctg gcggacgtcg tctggttaaa
    25141 cgcgagtacc cgttacgtga taccggcggt gtcaatgacc tggggaaaaa acttcgctcc
    25201 cgcacattca gcatctgcat tctgaacagc aacgcagaga ctgccagaga tgaagcgggt
    25261 gcgctgatgg atgctcttga tgcaccgggc agcggcgaac tggtgcatcc agagttcggc
    25321 actgtggacg tcatggtgga ttcatgggaa tgccgtacta aagcggatga actgaattat
    25381 tacgcgttca ccgttaccgt ttatccgtcg ttgcaggata cagccccgga cgcagaaaca
    25441 gacaccagta cagccgtatc tgcacaggcc gttgctgtaa ctggttcgct gggggatacg
    25501 ttgtcctctg tctggcagac cgtaaaagat ggcacagcgg cggcaacagc cgtaatggat
    25561 gccgtaaccg gcgtcatcga tgatatctgt gatgcggtgg ataatctggg gatcacccag
    25621 actatcagcg gcctgatggg gtcactttct gcaatgaaag gttctgtgac cggcctgata
    25681 aataaaccag ccatgctgac atcttcactg atgggggcgt tatccggcgt ttcatcgtta
    25741 tgcgataccc ggacagcatt ttccacctgg aaccgtctgg cgcagcggtt cgaaagccga
    25801 catgcctcca ccactgacag acaaggtgcc attaccactt catacagcag cccggttgca
    25861 gaaaaaaata ttgccacact gaactacgtc atgctggcag cagcacagac ataccgggcg
    25921 gaggcagcaa gccaggcact gactgcggca ctggattcca gccgtcagat ggataatgcc
    25981 gcccgtgcac cggcgctgga tgtatcagtc acaacaaccg gcatcgccag tggtgccagc
    26041 agcacatcgt ccacggtcac acaaagccag ttgcagttaa ccgggataac cccggacggc
    26101 ggcttttcac cagtatcctt ttcagacagt agcacagcca cgcccccggt atttgaaagc
    26161 gtgtccgata tcgaaaaaac aactgccatg ctgggggcgg cgctggatag cgtcattctg
    26221 acggcatctg agcagggttt ttcgacagac agtgttcagc ttacgcaact gcgtctgctg
    26281 gttgttgccg acctggaaaa acgcgggctg caactggcgg gtagtgaatc acaccacctg
    26341 ccggaaacgc tcccggcaat ggtggcactg taccggttca ccggaaacag ccggaactgg
    26401 caacggctgg cccgcaggaa cggtatcagc aacccgttat ttgttcccgg tggtgtcagt
    26461 attgaggtga ttaatgagta ataccgttac actgcgagcg gatggcaggc tgtttaccgg
    26521 ctggacgtca gtctctgtca cccgctccat tgaatccgta gccggatatt ttgagctggg
    26581 ggtgaatgtg ccaccgggta cagatttatc cgggctggca ccggggaaag cgttcacgct
    26641 ggaaatcgac gggcagattg tctgcaccgg ttacatcgat tcgcggcgac gccagatgac
    26701 cgctgacagc atgaaaatca ttattgccgg gcgcgacaaa acggctgacc tgattgactg
    26761 tgcagccgtt tacagtggcg ggcagtggaa aaaccgcaca ctggaacaga ttgcgcgtga
    26821 cctgtgcgct ccttatggcg ttaccgttcg ctgggagctt tccgataagg aaagttcggc
    26881 agcttttccc ggcttcacgc tggaccattc agaaaccgtt tatgaggcgc tggtgcgtgc
    26941 ctcccgcgca cgcggtgtac tgatgaccag caatgccgca ggagagctgg tattcagccg
    27001 ggctgccagc acagccactg atgagctggt tctcggagaa aatctgctga cactggattt
    27061 tgaggaagac ttccgcgacc ggttcagcga atacaccgtc aaggggtatg cccgcgcaaa
    27121 cggtgctgag ggtgatgata ttgatgcgaa aagtatcgtc tcccggaaag ggaccgccac
    27181 tgacagtgat gtgacccgtt acagaccgat gatcatcatt gccgacagca aaattacggc
    27241 gaaggatgca caggcccgcg ccctgcgtga gcaacgccgc agactggcaa aatccatcac
    27301 ctttgaggca gaaattgacg gatggacacg caaggacggg caactctgga tgccgaacct
    27361 gctggtcact attgatgcct cgaaatatgc catcaaaacc acagaattac tggtcagcaa
    27421 agtcaccctg atactgaatg accaggacgg gctgaaaacc cgcctcagcc ttgcaccacg
    27481 cgaaggcttt ctggtgccgg ttgaaagcga ccgtaaaaac aggaaaggcg gcgacagtaa
    27541 cggcggtatt gatgcgctgg ttgaagatta ttatcgcaga cacccggaga aaacgccgcc
    27601 gtggaaagag taaatgattc cgccatgaac cgcctgttga caccgctgat gcgtcgtgtg
    27661 cgtctgatgc ttgcgcgtgc cgttgttaac gtgattaacg acgggcgaaa ggttcagaac
    27721 ctgcaggtcg gtctgctgga tgatgaggaa tctgatgaag tggagcgcct gcaaaattac
    27781 gggcatttca gcgttcccct gccgggcgca gaggcgctga ttgcctgtgt gggtgcacaa
    27841 cgtgatcagg ggattgccgt tgtggtggaa gaccgccgct accgcccgac aaatcttgaa
    27901 ccgggtgatg caggcattta ccaccacgag gggcatcgaa tcaggctgac aaaggacggg
    27961 cgctgcatca ttacctgtaa aacggttgag gtttacgctg atgaaggtat gaccgttgat
    28021 acacccagaa ccacatttac cggcgacgtt gagatccaga aaggtctggg cgttaaaggt
    28081 aaaagccagt tcgacagcaa tattactgcc ccggatgcca tcatcaatgg caaatcgacg
    28141 gataagcata tccaccgcgg cgacagcggc ggaaccacgg ggccgatgca atgaccgatt
    28201 tagccattat ctggacgaac ggacgcggcg atattgcaca ggatggcatt gatatgctga
    28261 ccgacgacag cctgacaacc gatgtgacaa tctccctgtt tacagaccgg cgcgcgctgg
    28321 attctgacac gctgccggat ggttcagata atcgccgtgg atggtggggg gacagttacc
    28381 gcgaccgccc catcggttcg cggttgtggc tgttgtcacg tgaaaaagcc acgccggata
    28441 cgctggaacg cgccagaggg tatgccgaag aggcgctgga atggctgaaa acagcgggcc
    28501 gggtaagtgc gattaacgtc agagcggaac agttacatca gggctggtta tacctctaca
    28561 ttgcactgac attaccggat ggttccgtta ttccttatga gtttaaagca gcatttaacg
    28621 gggtttaaat ggcttattca ccaccgacat tatcatcgct gattgcccgt acagaacaga
    28681 atattgaaca gcgcctgccg ggtagctggc ctcaggcccg tgaaaaaacg ctgagtgcca
    28741 ttgcttacgc ccaggcaggg cttgctgccg gttgtcacga gcatatttcg tgggttgggc
    28801 ggcagattat cccgtcgaca gcagatgaag atgagttgct ggagcactgc cggttctggg
    28861 gtgtgcgccg caaacaggcg acagccgcca gcggcccgct gactgtcacc acatcggcag
    28921 cgacaaccat tcctgccggt acacgctggc agcgtgctga tggtgtggtt tacagcctgg
    28981 ctgatgccat tgtgattgac catgcaggaa cgacggaaat taccgttacc gcactggctg
    29041 ccggtgaggc aggaaatacc ggtgagaata cccttttaac gttgatcacc ccggttgcct
    29101 gtgttgtttc cgatgccatc actgtaaaag ggttttccgg tggggctgat attgagagtg
    29161 cagcggagct gctgtcacgg ctggaatatc gtgtccagta ccctccgttc ggcggtaatc
    29221 agtttgatta tgttcgctgg gcacgtgaag tcagcggcgt tacccgtgcc tggtgttttc
    29281 cgacatggaa aggcggtggc acagtcgggg tgacgtttgt tatggataac cggagcaata
    29341 tttttccaca accggcagat gtggaacgcg tggcggatta tatcgccggt catacagacc
    29401 cgataaccgg cctgattgtc ggacagcctg acggtgtaaa tgtcacggta tttgcgccaa
    29461 aggcaaagcc ggtaaatcca cggatttata tatcaccgaa gacagccgaa ctgaaacagg
    29521 ctattaccca cgccattaat actatgtttt ttaatgaagt gacgccgggc ggcgcacttg
    29581 ctccctcccg tattatccgt gcggtggcag gtgttaccgg tctggatgat tttgaagtgc
    29641 gtttcccgac agagatccag cgttcggaga atacggaact gttaacaccg gggacaattg
    29701 aatggctgta acaccctatc agacggcctt tctgcaatta ctgccgtcag ggcttgcctg
    29761 gaataaaagc cccgacagca aattatctgc gctggcgcag gccatcagtg acgtgattgc
    29821 cactgcggcg gatgatgcgc ggcaaatgct gcgggagcgt ttcccttcca catcccgctg
    29881 gtatctgggg gagtgggaat catttctggg gttgccggac tgtaccagcg aaaacggcac
    29941 cctgtcagaa cgccagcggg ctgccgcaaa taaaatgcgt atgaccggca atctgagcag
    30001 gcgcttttat gaatggctgg ctgcgcagta cggttttacc gtcaggctga cggattccac
    30061 agaaggccag tgggttacgc aggtcaatat ttacggtatt aaaaattatc gcaacgcaac
    30121 ggtgctggat aatgttctga cgccgttacg tgtttatgaa tcgggtgcgc tggagtgttt
    30181 actggagaaa tataaacccg cgcatcagat ttataaattt gtttaccatg acggagataa
    30241 ctaatgtttt atattgataa tgacagcggc gtaaccgtca tgccgcccgt atccgcccag
    30301 cgtagtgcta tcgttcgctg gttttcagaa ggtgacggga ataatgttat cacatggccc
    30361 ggcatggact ggtttaatat tgtgcaggcg gagttattaa acacgctgga agaagccggt
    30421 attcaaccgg ataaaacaaa attaaaccag cttgcactgt ccattaaagc catcatgaac
    30481 aagaatgcgc tgctgataaa aaataacctc agcgaaatta aaacagccgg ggcatcagca
    30541 cagcgtacag cacgtgaaaa tctggatatt tatgatgcca gcctgaacaa gaaaggactc
    30601 gtccagctaa ccagtgcaac tgacagctcc agtgaaacgc tggcagccac cgcaaaagca
    30661 gtgaaaattg cgatggataa tgccaatgcc cgactggcaa aagaccggaa cggagcagat
    30721 attcccaata agccgttgtt tatccaaaac ctcggtttac aggaaacggt aaacaaggct
    30781 gctaacgccg ttcagcgttc cggcgataaa atgaccggag aactgaaaat tggcacgatg
    30841 aatgcgctgc gaatttttaa tgatgccttc ggtcttattt tccgtcgttc agaagagtcc
    30901 cttcatttca tccctacggc tgaaggacaa ggcgaaaacg gtgatatcgg cccattaagg
    30961 ccattcgcta taaatctgag aacaggtgct atatctgtca gccacggggc caaaattgat
    31021 agtgggctgg cgcttggtac agataacgca ctgggcggta attcaattac tctcggagat
    31081 aacgacacgg gtattaaaca gggcggcgac ggtgtccttt tattctattc aaatggacaa
    31141 ctggcatttg ggctccaacc cgcatctgct gatttttata agcgggttgc atatattcat
    31201 cagggaataa ctcctgatgg aagtggcgca tttgcagacc agttaaacaa tgccaccgcg
    31261 ccttttgttc agacgcggtt tgcctggaat cccgcgcctg gcggtcatta cgtaccaata
    31321 gctaagggcc tgtctattcg caatggacag gggtatccgg gtgctgtcag ctttggctat
    31381 ttactgacag aacaaatggg atttccaatt ccatgtgttc atatgcgtgg cgatggcggt
    31441 aatgatgctt tatggcagtt taacccaaac gacaaatctt ttatttcacc gggctctctt
    31501 gttgcgggcg gagtccgtta taacaccgat ggaaatatat ttggcgggtg ttggggttca
    31561 aatttagctg attatctcaa tagcacatat gtaagagaca ttcgacttgg tagcgcagaa
    31621 tcaatatcag catggcgagg ccctggttac tgggatactt ctggatatgt tctgaccgca
    31681 gccggaaaca gcaacaccga tgagtttatt gatacattaa ccagaagacc tattcagaag
    31741 ttgattggtg ggacatatta taacgttgtg agtatttgac atgatgcatt taaaaaacat
    31801 taaggcagga aatccaaaaa caccagaaca ataccagtta accaaaaaag ctggagttgt
    31861 ctggctttat actgaagatg gtaaaaactg gtacgaagaa caaaaaaact ttcaaccaga
    31921 tactataaaa attgcatacg ataaaaataa tgtaattgtt accgtgagca aagatgtttc
    31981 aactattaac ccggaaggtt taagtgttgt tgaagtatcc gatattacgg caaatcgtcg
    32041 cgcagataat tacggaaact ggatgtttct gaatggtaaa gtggtaaaac gggaatatac
    32101 agaacaggaa ctgcaacagc aggctgagtt acaaaaagcc gctttgcttt ccgaagcgga
    32161 gtctgtgatt caaccgctgg aacgtgctgt caggctgaat atggcaacag atgaggagcg
    32221 cacacgactg gaagcatggg aacgctacag tgttctggtc agccgtgtgg atacggcaaa
    32281 gccagaatgg ccacaaaaac cagagtaaaa aataaggccc gataggggcc ttctctcatt
    32341 ctggttgttc gggaaacgtt actggcaggc tggaggtgtc tgtagattcg actttctgcg
    32401 caaagagcat ccactctgtt aatttttgtt tattctcgtc ggaaatgatg cccagccgta
    32461 gctgtgagtc ccatagctgg gttttatccc tgacaagttg caacaggctt tgcttttcat
    32521 tttccgcttg ttgcctcttc tcttcctcgg tgtaagttcg ctttatcact acgccatctt
    32581 tgaacatcca tttccccgaa atatcagccc ggcgatttgc tgtaatatca ggtaattcaa
    32641 cgacgcttgc accttctgga ttaattgctg aaacatcttt ttcaatgcaa ataataacgc
    32701 cgttatggtc atagaccatt ttcaaagtgt ctggttggaa attcttttgt tcctcgtacc
    32761 agtttctccc atcatcggaa taaagccact taatattgaa ttgttttgtt agctggtatt
    32821 gctccactgt tttaggattg ccagcagtaa tattttttaa gtgcatcata attaaatact
    32881 ccccacgtta taccaagtcc cattaatgca atactgaatt ggccttgcct gagttgtatc
    32941 aattagttca tctctatttg agttaacgga accggtaaca acataacctg acttatcaga
    33001 ccaaccaggt ccattccatg tctgaacaga tgataggcct cctaaacgga cgcctgtaat
    33061 aaatcgtgaa ttacattctg ctttggtata tgcaccaata tctcctgctg atggtttgtg
    33121 agcggttgta tatagttgcg cccatccaga ccaattagca tctgctgtat ctcgtcggga
    33181 gcgaatatat gccggagcat gaccgccgct ggtcccactc cacccaataa ataactctcc
    33241 ttcgccaact gccgctacgc cagtaagatg aagaacattg ccataagtaa aaggatatcc
    33301 attgttgtac gcttcataca tttggatacc aggcgtaccc tttgcgctcc cacctaatgc
    33361 tgtaacacgg ttacgagata ctaacgtatt aatatttata tcgctggaac catcaaattt
    33421 aactccatta atatttcttg ctgttttcag ttttgtcgca gtagcggcat tgccggacag
    33481 ttccccggaa aggccgccgt tgaatgtttg tcgattagtc caggtatttg ctgtactgag
    33541 taacggtatt ttttctccgc ttgtgccgag ttctcgtaaa ccgaggtttt agataatggc
    33601 cgtttctggc ctgcatggca tgatttgcgc ttttaaacgg gagatccaga gtgctgattg
    33661 gctatgtaag ggtatcaaca aatgaccaga atacagacct gcaacgaaac gctcttgttt
    33721 gtgcaggatg tgaacaaata tttgaagata aattaagcgg aacaaggaca gaccgaccgg
    33781 gattaaaacg tgctttaaag cgccttcaaa aaggtgacac actggttgtc tggaaactgg
    33841 atcgcctcgg gcgaagcatg aaacatttga tttctctcgt aggggaatta cgagagcgag
    33901 ggattaattt tcgcagtctt actgacagta ttgatacgtc atctccaatg gggcgttttt
    33961 tcttccacgt gatgggtgcc ctggctgaaa tggaacgaga actaatcatc gagcgaacga
    34021 tagctggact tgctgccgcc agaaataaag gccgtattgg tgggcgacca cctaaactaa
    34081 ccaaagcaga gtgggagcag gccgggcgtt tattagcaca aggaatcccc cgcaagcagg
    34141 tcgcattgat ctacgatgtg gccctctcaa ctctgtataa aaaacaccct gcgaaacgaa
    34201 cgcatataga aaacgacgat cgaatcaatc aaatcgatcg gtaatacaga tcgattatgc
    34261 cctaataacc acacttaacc cacgatgttt tttaagatag tggcgaattg atgcaaagga
    34321 ggtgagatga aatcaattcg ctgtaaaaac tgcaacaaac tgttatttaa ggcggattcc
    34381 tttgttcaca ttgaaatcag gtgtccgcgt tgcaaacgtc acatcataat gctgaatgcc
    34441 tgcgagcatc ccacggagaa acattgtggg aaaagagaag aaatcacgca ttctgacgaa
    34501 accgtgcgtt attgagtatg aaggccagat tgttggctat ggttcaaagg agctgcgcgt
    34561 tgaaaccata tcctgctggc tagcccgcac aattattcag acaaagcact attcccgccg
    34621 ttttgtgaat aactcttacc ttcacctggg ggtattcagc ggacgcgatc tggttggcgt
    34681 tctccagtgg ggatatgccc ttaaccccaa ctcaggtcgt cgtgtcgtgc ttgaaacgga
    34741 taaccggggc tatatggagt tgaaccgcat gtggctacac gacgacatgc cccgcaactc
    34801 tgaatcacgg gccattagct acgcgctgaa agttatcaga ttactgtatc cgtcagtgga
    34861 gtgggttcag tcctttgctg acgaacgctg cggacgcgca ggcgttgtgt atcaggcgtc
    34921 gaattttgat tttattggca gtcatgaaag tacgttctac gagctggatg gtgagtggta
    34981 tcacgagata acgatgaacg cgattaagcg aggtggacaa cgaggcgtgt atttacgggc
    35041 taataaagag cgtgccgtgg tgcacaaatt taatcagtat cgctacatca gattcctgaa
    35101 caagcgagca aggaagcggc taaataccaa actattcaag gttcagccat accctaagtg
    35161 atccccgtgt aatgaataaa aagcagtaat taatactgct gtttcatttg aagcgcgaaa
    35221 gctaaagttt tcgcatttat cgtgaaacgc tttcgcgttt ttcgtgcgcc gcttcactcg
    35281 ggtcgatgat ggggccaacg attgtgtttt ccattccggt ggcgctcggc attatcgaac
    35341 cttctgaccg tcgttatctg gcgctcggcg tgctggcggg cattgtgacc attccgattg
    35401 gttgtatcgc tggtggtctg gtagctatgt actccggtgt tcagatcaac ggccagccgg
    35461 tggaattcac tttcgccctg atcctgatga acatgatccc ggtgatcatt gttgcgattc
    35521 tggtggcgct ggggctgaaa ttcatcccgg aaaaaatgat caacggcttc cagatcttcg
    35581 ccaaattcct cgttgcattg atcaccctcg gtcttgccgc tgcggtagtg aaattcctgc
    35641 ttggctggga actgatcccc ggtctggatc ctatctttat ggcccctggc gataaacccg
    35701 gtgaggtgat gcgcgccatt gaagttatcg gttctatctc ctgcgttctg ttaggggcgt
    35761 atccgatggt gctgctgctg acccgctggt ttgaaaaacc gctgatgagc gtcggtaagg
    35821 tactgaatat gaacaacatc gcggcagccg gcatggtggc aacgcttgcc aacaacattc
    35881 cgatgttcgg catgatgaag cagatggata cccgcggcaa agtgatcaac tgcgccttcg
    35941 ccgtttccgc ggctttcgcc ctgggcgacc acttaggctt cgccgctgcc aacatgaacg
    36001 ccatgatctt cccgatgatt gtcggcaaat tgatcggcgg cgtaacggcg attggcgtgg
    36061 cgatgatgct ggtgccaaaa gaagacgcga ccacggctaa aaccgaagcg gaggcgcaat
    36121 cgtgaacact cgccagctat tgagcgtcgg tatcgatatc ggcaccacca ccacccaggt
    36181 gattttctcc cacctggagc tggttaaccg tgcggcggtg tcgcaggtgc cgcgctacga
    36241 attcattaaa cgcgaaatta gctggcaaag tccggtgttc tttacccctg tcgataaaca
    36301 gggcggttta aaagaagcgg aactgaaaac cttaatactc gagcaatatc aggctgcggg
    36361 tattgagccg gaaagcgttg attctggtgc catcatcatc accggtgaaa gcgcgaaaac
    36421 ccgcaatgct cgcccggcgg tgatggcgct ctctcagtcg ctgggcgatt ttgtcgttgc
    36481 cagcgccggg ccgcacctcg aatccgtgat cgccggtcac ggagccgggg cacaaaccct
    36541 ttctgaacaa cggctgtgtc gggtactgaa tatcgacatc ggcggtggca ccgcgaacta
    36601 cgccctgttc gatgccggaa aaatcagcgg caccgcctgc ctcaacgtcg gtggtcgcct
    36661 gctggaaacc gacagccagg ggcgcgtggt ttacgctcat aaaccggggc agatgattgt
    36721 ggatgagtgc ttcggtgcag gcactgacgt ccgttcgctg accggcgcgc agctggtgca
    36781 ggttacccgg cggatggcag aactgattgt cgaagtgatt gacggaacgc tctcgccgct
    36841 cgcgcaggca ttgatgcaaa ccggtttgct gcccgcaggt gttacgcccg aaatcattac
    36901 gctttctggc ggcgtgggcg aatgttatcg ccaccagccc gccgacccgt tctgttttgc
    36961 cgatattggc ccgctgctgg caacggcgct gcatgaccat ccgcgcctgc gtgagatgaa
    37021 cgtgcagttt ccggcgcaaa ccgtgcgtgc cacggtgatt ggcgcggggg cacataccct
    37081 ttcgctctct ggcagcacaa tctggcttga gggcgtacaa ctgccgctgc gcaatttgcc
    37141 ggtggcgatc ccgattgatg aaacggatct ggtgagtgcc tggcaacagg cgctgattca
    37201 actggatctt gatcccaaaa ctgacgcgta cgtgctggcg cttcccgcct cgctgcctgt
    37261 gcgttacgcc gcggtactga cggtcatcaa cgcgttggtc gatttcgtcg cgcgttttcc
    37321 gaatccgcat cccctgctgg tggtggccgg gcaggacttt ggtaaagctc tgggcatgtt
    37381 gttgcgccca cagctacaac aactcccgtt ggcagtcatt gacgaagtga ttgtccgcgc
    37441 gggggactat atcgacattg gtacgcctct ttttggcgga tcggttgtgc cggtgacggt
    37501 gaaatcactc gcatttcctt cctgagggaa cgacttatga aactaaagac cacattgttc
    37561 ggcaatgtat atcagtttaa ggatgtaaaa gaggtgctgg ctaaagccaa cgaactgcgt
    37621 tcgggggatg tgctggcagg cgttgcagcg gcaagttcac aggagcgcgt ggcggcaaag
    37681 caggtgttgt cggaaatgac cgtagcggac atccgcaata atccggtgat tgcctatgaa
    37741 gatgattgcg tgacgcggct gattcaggac gatgttaacg aaacggccta caaccagatt
    37801 aaaaactgga gcatcagcga actgcgtgag tatgtgctga gcgatgaaac cagcgtggac
    37861 gacattgcct ttacccgcaa agggctgacc tcggaagtgg tcgcggcggt agcgaagatt
    37921 tgctccaacg cggacctgat ctacggcgcg aagaaaatgc cggtaatcaa aaaggccaat
    37981 accaccatcg gtattccggg caccttcagc gcccgtttgc agccaaatga cacccgtgac
    38041 gacgtgcaaa gtatcgccgc gcaaatctac gaagggcttt ccttcggggt gggcgatgcg
    38101 gtgatcggcg tcaacccggt aactgacgac gtggaaaact taagccgcgt gctggacacc
    38161 atctatggcg tgatcgacaa attcaacatc ccaactcagg gctgcgtact ggcgcacgtc
    38221 accacccaga tcgaagcgat tcgtcgtggc gcaccaggcg ggctgatttt ccagagtatc
    38281 tgtggcagtg aaaaagggct gaaagagttt ggcgtggaac tggcgatgct cgacgaagcg
    38341 cgcgcagtgg gtgcagagtt caaccgtatc gctggggaaa actgcctcta cttcgaaacc
    38401 ggacaaggtt cagcgctatc cgctggcgct aacttcggcg cagaccaggt gacgatggaa
    38461 gcacgtaact atgggctggc gcgtcattac gatccgttta tcgtcaacac cgtggtcggc
    38521 tttattgggc cggagtatct ctacaacgac cgccagatta tccgtgcggg cttagaagat
    38581 cactttatgg gcaagctgag cggcatctct atgggctgtg actgctgtta caccaaccac
    38641 gctgacgctg accagaacct caacgaaaac ctgatgatcc tgctcgccac cgcaggctgc
    38701 aactacatca tggggatgcc gctgggtgat gacatcatgc tcaactacca gaccaccgca
    38761 ttccacgata ccgccactgt gcgtcagtta ctcaacctgc gtccgtcacc ggagtttgaa
    38821 cgctggctgg aaagcatggg cattatggca aacggtcgcc tgaccaaacg ggcgggcgat
    38881 ccgtcactgt tcttctgatg acgcggagat aacacatcat ggatcaaaaa cagattgaag
    38941 aaattgtacg cagcgtgatg gcgtcaatgg gacaaacggc ccccgcgccg tcagaagcaa
    39001 agtgcaccac caccacctgt gcggcaccgg tgacctcgga aagctgtgcg ctggatttag
    39061 gttccgcgga agcaaaagcg tggattggtg ttgaaaaccc gcatcgcgca gacgtattaa
    39121 cagaactgcg ccgcagcacc gtggcccgcg tttgtaccgg tcgtgccggt ccgcgtccgc
    39181 gtacccaggc gttgctgcgt ttcctggccg atcactctcg ttcgaaagat accgtactaa
    39241 aagaagtgcc ggaagagtgg gtgaaagcac aaggcttgct ggaagtccgc tccgaaatca
    39301 gcgacaaaaa tctctacctg actcgcccgg atatgggccg tcgcctgtgt gcagaagctg
    39361 ttgaagcgct gaaagcgcag tgtgttgcca atccggacgt gcaggtcgtt atttctgatg
    39421 gcctgtcaac tgatgccatc actgtgaact acgaagagat cctgccaccg ctgatggcgg
    39481 gcctgaaaca ggccgggctg aaagtcggta caccattctt tgttcgttat ggtcgcgtga
    39541 agattgaaga tcagatcggt gagatcctcg gcgcgaaagt agtgatcctg ctggtgggcg
    39601 aacgtccggg gttgggtcag tcagaaagcc tctcctgcta cgccgtttac tcgccgcgta
    39661 tggcgaccac cgttgaggcc gatcgcacct gtatctctaa cattcaccag ggcggcacgc
    39721 cgccggttga agccgcagcc gtcattgtgg atttggcgaa acgtatgctg gagcagaaag
    39781 catccggcat caacatgacc cgataaggag gcatcatgcc agctttagat ttgattcgac
    39841 cgtctgtcac cgctatgcgg gtgattgcct ccgttaacgc cgattttgcg cgtgaactga
    39901 aattgccgcc gcatattcgt agcctcggtc tgatttctgc cgactccgat gacgtcacct
    39961 acattgccgc tgatgaagcg accaagcagg cgatggtgga agtggtgtat ggccgctcgc
    40021 tgtatgcggg cgctgcacac ggcccgtcac cgactgccgg tgaagtgctg attatgcttg
    40081 gtgggccaaa cccggcggaa gtgcgtgcag gtctggatgc gatggttgcg catattgaaa
    40141 atggcgcagc tttccagtgg gctaatgacg cgcaagatac ggcattcctg gcacatgtag
    40201 tttcgcgtac cggttcttat ctctcatcaa ccgccgggat cacgcttggc gatccgatgg
    40261 catatctggt ggcaccgccg ctggaagcga cctacggcat tgatgcagcg ttgaaatctg
    40321 ccgacgtaca gctggtgacc tatgtcccgc caccgtctga aactaactac tcggcagcat
    40381 ttttaaccgg tagccaggca gcgtgtaaag cggcctgtaa cgcctttacc gatgcagtgc
    40441 tggaaatcgc gcgtaatcca atccagcgtg cgtaatggag gttgccgatg atcaatgcac
    40501 tgggattgct ggaagtggac ggcatggtcg ctgcgataga tgcagcggat gccatgctca
    40561 aagcagctaa cgttcgtctg ctcagtcacg aagtgcttga ccctggtcgc ttaacgctgg
    40621 tggtggaagg cgatctggcg gcgtgtcgtg cggcgctgga cgcaggttgt gctgccgcga
    40681 tgcgtaccgg gcgtgtcatc agccgtaagg agatcggtcg gccagacgat gacacccagt
    40741 ggctggttac tggctttaac cgccagccga agcaacccgt aaaggaaccc gacgcgccag
    40801 ttatcgtcgc ggaatctgct gacgagttgt tggcgctgtt aacatcagta cgtcagggaa
    40861 tgacggcagg agaagtggct gcccactttg gctggccgct ggaaaaagcc agaaatgcgc
    40921 tcgaacagct cttttctgcc gggacgttac gtaaacgcag tagtcgttat cgtctcaagc
    40981 cccattaacc tgtcggaggt gccgggtgtc ctacaacacc cggcattaac atcatgaaaa
    41041 agacccgtac agccaatttg caccatcttt atcatgaacc cttacccgaa aacctgaagc
    41101 tcacgccgaa ggtcgaagtg gataatgttc atcaacgaca gacaacggat gtctatgaac
    41161 atgctttgac gattaccgcc tggcagcaga tttacgatca gctgcatccg ggcaagtttc
    41221 atggtgaatt tacggaaatt ctactcgatg atattcaggt ttttcgtgaa tacaccggtc
    41281 tggcgctgcg tcagtcgtgt ctggtctggc cgaactcgtt ctggtttggc attccggcga
    41341 cgcgcggtga gcagggattt atcggttcgc aatgtctggg aagcgcagaa atagcgacgc
    41401 gccccggtgg aaccgagttt gaattaagta cgccggacga ttacacgatc ctgggcgtag
    41461 tgctttctga agatgtcatc acccgtcagg ctaacttttt gcataatccg gatcgggtat
    41521 tacatatgtt gcgtagccag tcggcgctgg aagtgaaaga gcagcataaa gccgcgctgt
    41581 ggggctttgt ccaacaggcg ctggcgacgt tttgcgagaa cccggaaaat ctccatcagc
    41641 ctgcagtgcg aaaagtgctg ggggataatt tgctaatggc gatgggggcg atgctggaag
    41701 aagcgcaacc aatggtgacg gcggaaagca tcagtcatca gagttaccgt agattacttt
    41761 cccgcgcccg tgaatatgtg ctggaaaaca tgtccgaacc ggtgacggtg ctggacttgt
    41821 gtaatcaact gcatgtcagc cgccgcacgc tacaaaacgc gtttcacgct attttaggca
    41881 ttggcccgaa cgcgtggctg aaacgcattc gcctgaacgc cgtacgccgc gaactgataa
    41941 gcccgtggtc gcaaagcaca acggtaaaag acgccgccat gcagtgggga ttctggcatc
    42001 tggggcaatt tgccacggat taccagcagc tgtttgccga gaagccgtcg ttgacgttgc
    42061 atcagcggat gcgggaatgg gggtgaggga gttacaccca atccctgacc ttaataaact
    42121 cacttaacgc cgcttctggg ctgccatctt ttggctgata atcatactcc cagcgtacca
    42181 gtggcggcat tgacatcagg atcgactcgg tgcgcccgcc agtttgcagg ccaaacagag
    42241 tgccgcgatc ccagaccaga ttgaactcga cataacgacc gcgacggtag agctgaaaat
    42301 tgcgctcgcg ctcgccgtag gccatcgctt tacgtcgctc tacaattggt aaataagcgt
    42361 cggtgtagcc tttgcctacc gcctgcataa aggcaaaaca gtggtcgaaa tctggcgtgt
    42421 ttagatcatc aaagaacagc ccgccaatac cgcgctgttc gttgcgatgt ttgaggtaga
    42481 agtaatcgtc gcaccacttt ttgtaacggg tataaacgtc ttcaccaaat ggcaggcaca
    42541 ggtcacgggc ggtgcggtgc cagtgaatgg cgtcttcttc aaaaccatag aaaggggtta
    42601 aatcgaagcc gccgccaaac caccagacgg gctctgcacc cggtttttcg gcaataaaaa
    42661 agcgcacatt cgcgtggctg gtgggaacat acgggttatg cggatgcact accagagaaa
    42721 cgcccatcgc ctcgaaactg cgcccggcaa gttccgggcg atgagcggtg gcggaagcag
    42781 gcatcgcctc gccgtggacg tgagaaaagt tgacgcctgc ctgttcgaaa acaccaccat
    42841 tacgcaacac ccgactacgc ccgccgccgc caccttcgcg ctgccagcta tcttcgacaa
    42901 attctgcgcc atcgacggcg gtcagctgct gacaaatcgt atcctgaagg ttgagcagaa
    42961 actgtttaac ctggtgtgcg tcgggtttca taacttatcg ctttttcgaa tgtgctttct
    43021 ggttgtcgaa ccagtggaaa taactgatca cgccttcagc aatcgctgtg gcgattttct
    43081 gacgaaacgc cgccgtgcct aacagtcgct cttcttccgg gttggtgata aacgaggttt
    43141 ccaccagcac cgaaggaacc gacggcgatt tcaacaccac aaacgcggct tgttcggtgt
    43201 tgcggctgtg cagtttatgc accggcttaa tctttttcag aatatgcgag ccaagcgtca
    43261 ggctgttttt aatagtatcg gtttgcacca gatcaaacag cacttgctgc aatagatgat
    43321 ccttatcagt cgccttttta cctgcaactt catcggcgcg gttttcgcgt tcagacaggt
    43381 atttcgccat tgcgctactt gccccacggt tagagagggc aaataccgaa gcaccggcag
    43441 ctttcgggtt ggtaaagcca tcggcatgaa ttgacataaa cagatctgcg ccatgtttat
    43501 gggcgatttc aacgcgatcg taaagtggga taaacgtatc gccagaacgc gttaaacgcg
    43561 catcaatccc atgattacgc aaaatggaac ggacgttttt agcaatcgcc agcaccacat
    43621 gtttttcttt cgaaccgttg cgcccgatcg ctccggtatc aatcccgccg tggcctggat
    43681 cgagaacaac gacacgtttg ccgccagatt ttttagcttt cggcttgctg tgtccgttgc
    43741 tggtttttaa aggttcgtct ttggcgatgg cttgcgacat tcctgacaac gtcagggcag
    43801 ccaaaccggc tttcagcacc tggcggcgcg aagtgagtgt ttttagtggt ttaaaagtgc
    43861 tcatacggcc tgagttgtaa taataaagtt ccagatgtta tatcgtatcg cgtaatccgt
    43921 tacgaccgtt tgctgttgag aattgtttta cttttcattt caatagttga caactcccca
    43981 ttatgccact ttttcaaccg attttcgtca gatattggct aattcgaccg ttcacgccat
    44041 aatcagcctt ttaacccgca aaaaggtaac gaataccatg gagatacgcg tatttcgcca
    44101 ggaagatttc gaagaggtca tcaccctttg ggagcgttgc gacttgctgc gtccgtggaa
    44161 cgatccggaa atggacatcg agcgtaagat gaaccatgac gtcagtttgt ttctggtcgc
    44221 tgaggtaaac ggtgaagtgg tcggaacggt gatgggcggt tatgacgggc atcgcgggtc
    44281 tgcttattat cttggcgtgc atccagagtt tcgtgggcgt ggaattgcca atgcgttgct
    44341 taaccggctg gagaaaaagc tgattgctcg cggctgcccg aaaattcaga tcaacgtgcc
    44401 ggaagataac gacatggtgc tcggaatgta tgaacgcctg ggatatgaac acgccgacgt
    44461 gctgagtctg ggtaagcgtt tgattgaaga tgaagagtac tgagtttcat cctgtccatt
    44521 atgatgcgca cggtcgcctg cgtttaccct tgctcttctg gcttgtgcta ttgcttcagg
    44581 cgcgaacctg ggtgctgttt gtcattgccg gtgcgtcgcg cgagcagggt actgcgctgc
    44641 taaatctgtt ttatcccgat cacgataatt tctggctggg gctgattcct ggcattccgg
    44701 cggtgctggc gtttttgttg agcggtcggc gggctacgtt tcctcgcacc tggcgtgtac
    44761 tctattttct gttgctgttg gcgcaggtgg ttttactttg ctggcaaccg tggctgtggc
    44821 tgaacggtga atccgttagc ggtatcggtc tggcgctggt agtggcggat attgtggcgt
    44881 taatctggct gctgaccaat cgacgtttac gcgcttgttt taatgaagaa aaagaataac
    44941 gcagaggtgc gctttgttta tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat
    45001 ttcgcaaatt caatatattg caggaaaaag taggcctgat aagcgtagcg catcaggcaa
    45061 ttttgtgttt gtcatcagcc tctggcggca cttttttgca aattagcact ccaagcaacg
    45121 ttattgaata accaaaggaa gtgacatgaa atcgctgcgt ttattgttat gcgctatgcc
    45181 gttgatgcta actggctgtt ccactatgtc gtcagttaac tggtctgccg ctaacccgtg
    45241 gaactggttt gggtcgtcca ccaaagtgag cgagcagggc gttggtgaat taacggcgtc
    45301 cacaccactg caagaacaag ccattgccga tgcgcttgat ggcgattatc gcctgcgcag
    45361 cggaatgaaa accgcgaacg gcaacgtggt gcgctttttt gaagtgatga agggcgacaa
    45421 cgtggcgatg gtgattaacg gcgatcaagg cacgatcagt cgcattgacg tgctggatag
    45481 cgatattcct gctgacaccg gtgttaaaat cggtactccg tttagcgacc tttacagcaa
    45541 agcatttggc aattgccaaa aagctgatgg tgatgataat cgtgccgtcg aatgtaaagc
    45601 cgaaggcagt caacatatta gctaccagtt cagcggggaa tggagtggtc ctgaagggtt
    45661 aatgccttcg gacgataccc tgaaaaactg gaaagtcagt aaaattatct ggcggcgtta
    45721 atttgcgtct gaacaaaccg ccgccgcgaa aaaacacgta aaatagcgct caacaatgcc
    45781 acggattgcg tggcattctt attttcagga ggaacaatgt ctcaggttca gagtggcatt
    45841 ttgccagaac attgccgcgc ggcgatttgg atcgaagcca acgttaaagg ggaagttgac
    45901 gccctgcgtg cggccagtaa aacatttgcc gataaactgg caacttttga agcgaaattc
    45961 ccggacacgc atcttggtgc ggtggttgcc tttggtaaca acacctggcg cgctctgagc
    46021 ggcggcgttg gggctgaaga gctgaaagat tttccgggct acggtaaagg ccttgcgccg
    46081 accacccagt tcgatgtgtt gatccacatt ctttctctgc gtcacgacgt aaacttctct
    46141 gtcgcccagg cggcgatgga agcctttggt gactgcattg aagtgaaaga agagatccac
    46201 ggcttccgtt gggtggaaga gcgtgacctg agcggctttg ttgacggtac agaaaacccg
    46261 gcgggtgaag agacgcgtcg cgaagtggcg gttatcaaag acggcgtgga tgccggcggc
    46321 agctatgtgt ttgtgcagcg ttgggagcac aacctgaagc agctcaaccg gatgagcgtt
    46381 cacgatcagg agatgatgat cgggcgcacc aaagaggcca acgaagagat tgacggcgac
    46441 gaacgtccgg aaacctctca cctgacccgc gttgatctga aagaagatgg caaagggctg
    46501 aagattgttc gccagagcct gccgtacggc accgccagtg gcactcacgg tctgtacttc
    46561 tgcgcctact gcgcgcgtct gcataacatt gagcagcaac tgctgagcat gtttggcgat
    46621 accgatggta agcgtgatgc gatgttgcgt ttcaccaaac cggtaaccgg cggctattac
    46681 ttcgccccgt cgctggacaa gctgatggcg ctgtaagcgt ttctgattcg atgatgaaag
    46741 gccaggcagt atcacaactg actggccttt ttattacttc tgctttaacg ccgcatacac
    46801 ttgatcaacc acccaaccat aagttgcaat cggcaactga ccgctttcaa acatattggg
    46861 atttttttgt ggatcggcta ctggcgaatg gggcttgttg cttaacatca caatcgccat
    46921 gtgattcacc ggatcgataa cggttaccgt cccggtccag cccgtgtgac cgtaggtttg
    46981 cgggcttgcc agcgtgccaa acgtcggtgt catggtggca ttaccattca cgcgccagcc
    47041 gaggccaaaa gtggcgtctt ccttagagct ggtggtgaac atcttcaccg tttccgcact
    47101 gaacagctgc acatcgccat agcccccgcc gttcagcatc gtttgcatta acaccgcaat
    47161 atcgccggta ttggaaaaca aacctgcgtg cccggaaacg ccgcccatcg aataaaaggc
    47221 tttttcatcg tgcacctgac cccagagagt ggaggtgcgg atattcggga agtggatcac
    47281 tccatcgcgg gtattgccgt tcaattccgt ggcggcaatt tgttgcggtt taaagccttt
    47341 cagcagcggg ttaaataccg tatgtgtcag gccgagcggg cgataaatcg actcttcaac
    47401 atagcggtca agcggctgac cggtgaccga ctcgacgata aatccaagca gcatataatc
    47461 gacatcactg taaatatgct tgctgccggg ctgatactct agcggcgtgc gcttgatcat
    47521 ctccagcgtc tggcctttat cctgggaata taacgcgccc gcgaccgctt tattcggata
    47581 ttgtgggtcc gccgggaaac cgccgctgtg atgcagcagg tcagaaatcc gcagcgtgtt
    47641 tttgcctttg atggtgtcat ttggactatc ggcaaaccct ggaatatact tcgcaatcag
    47701 atcgtcagga tgcagcttgc cttcgctcat tagcttttgc agagcaaagt tagtggcgta
    47761 catcttggta ttggaagcca ggtcatacag cgtcccggtg gtggctttga caggctgttc
    47821 catcaatacg ctgccatcgt actttttcgc cgctccccag gctttgcgat acacaatctg
    47881 attatctttt ataatcagca ggtttatgcc gggataaccg gcgtccactt gctggctaat
    47941 ccagcgatcc atctggttaa gccgttcgac gttgaaccct gctttctctg gcgaactttc
    48001 tgtcaaaaca gggtattttg cggcgctgac gctacaacta acagccagca gagaaagata
    48061 gagcattgtc cgtttcataa tatcagtcca gattgacgtt acggcagcca aaaagcgtgg
    48121 tgaaaataaa cccgcaaacc catgccacca gaatcccgcc agcgtagact gccatcgccg
    48181 gaagaatgcc ttgtgctgaa gtcatcagcg gcagcgccac cagaccagac ggcccaaagg
    48241 cgctgtttaa gcccatcggc agaccccacc aggctatcaa tccgataaac aaaccgcccg
    48301 ccgcgccgcc taaacaggcg gtaataaacg gtttcatgcg gggcagggtg acgccataaa
    48361 tcaacggttc gccaacgccc agcaggccgg gaataatcgc cccgcgtacc tgactgcgta
    48421 atgcactgtg cggttgcgcc cgccagtaga gtgctagcgc cgcgcccacc tggcctgcgc
    48481 ctgccatcga aaggatggga aataagctgt tgaatccctg gctgtccatt aacgcgaggt
    48541 aaacaggaat aaagccctga tgcacgccaa ataccacggc gatcaggaac agcccggcta
    48601 aaaccgcaca accgagcgga ttactgttca ggtgcataaa cagccacgac ataccttcga
    48661 ataaccagcc gcccagcggc ataatgatca ggtaggccag cgtagcggtg atcagtaggg
    48721 tgattaacga ggtcagcagc atgtcgagat cgtcgggcat aaagcggcgc accatgcctt
    48781 cgatgcgggc gcaggcccac gcggcaatca acacgccgat aatattgccg cgcggatcga
    48841 tgggcagacc aaagaaatcg tgaaaaccgg cgtagtaacc ggtggttgca gcggggttat
    48901 aaccgagcaa aaagagtgcg gcaataattg cgccattgac gcccgtgccg ccaaatgcct
    48961 gggcggcgtt atagcccacc agaatcacca ggaaggtgaa caaacctttg ctgaacacct
    49021 tcataaaatt cagcgcgtcg ggtagcgtgc cctgagcatc tgccggaacg tgcatcaccg
    49081 tggcaattaa cgtcgctatc cccagcagca gaccggcggc aataaaaccg gggataagcg
    49141 gcgtgaagat ggtggcgaat ttggcgagaa attgttgcac gccggaggtt tgtttggctt
    49201 ttaactgacg tttgttctgg gcggcgattt ctgcggcatc ctgtaccggt gcttctccca
    49261 gcagctcgct catcgctttt gcggcacgat gcgctttacc aggcccaaaa acgacctgca
    49321 cctggtcgct ggtcaaaatg acgcccttca cgccttccag cgttttgata ttgggatcaa
    49381 ccagtgaact gtcatgtaca cccagacgca ggcgcgtcat acagttacca caactggcga
    49441 tattgcccgg tccgccgaca cgggtaagaa tggtgttcag aagttcactg ctgatctctt
    49501 tggccatggg ttattccttg tctaaaacct gacgaataaa gccgccgtgt tgatccaggc
    49561 gttttttagc ttctgcggca tcgagatttt tcagcaccat cacaatggcc gttttacagt
    49621 tgcgctcgca ggcaattaac gccgcttccg cttgttcggc attacatccg gtggcgtttt
    49681 taacgatatt gacctggcgc acatgcagtt tttcgttggt ggcgaccaca tcgaccatca
    49741 ggttgccgaa cactttgccg gatttaatca tcagcccggt ggagagcata ttgagcacca
    49801 gtttctgcgc cgtacccgct ttcatccgcg aagaaccggt aaccacttcg gcacctacaa
    49861 tcggtgtaat ggcaaactct gcggtggttg aaacggcgct ccccggatta caggaaattc
    49921 ccactgtgcg gcagccgagc tggcgtgcgt attccagtcc ggcaattaca tacggcgtgc
    49981 gaccactggc cgcaatgcca accaccacat cctgcgcggt taagccgatg tttttcagat
    50041 cattaacacc gccttcccgg ctatcttcag cgccttccac cgcgtgctga atggcatatt
    50101 cgcctccagc aatcaaacca accaccagac ccggtttaac gccgtaggtg ggcggacatt
    50161 cgctggcatc cagaatcccc agacgaccgg atgttcccgc accgagataa atcagacgac
    50221 cgccgccgct tacctgggcg tggataacat cgatcgccgc ggcgatatcc ggcagtacgc
    50281 gctcaacggc aagcggtacg gttttatctt catcgttgat aatccggcac atttccagcg
    50341 ttgatacgcg gtcaatttca gccgaggcgg cgttcgagcc ttcggtaatc atcttttcaa
    50401 gttgcatcgg tggtgacctt actatttacg atcaaataat tgcagatgta tggaatcata
    50461 tatcccgaat tgacagactt aaaggaatca ttgagtctac actcgcagag tgtgaagagc
    50521 gtcataagaa cttaagcgga atttttgctg ccagcccagg agagtggaag atgttgtacc
    50581 tgacgaaaat acgcaatgcg gaaagtgaat ttaccgagaa cgagcaaaaa attgctgact
    50641 ttctgcgggc caacgtgagt gagctgaaat ccgtctcttc gcgcaagatg gcgaaacagc
    50701 tcggcatcag ccagtcgagt attgtgaagt ttgcccagaa gctcggcgca caaggattta
    50761 ctgaattgcg tatggcgtta ataggcgaat acagtgccag ccgggagaaa acgaatgcca
    50821 cggcgctgca cctgcacagt tcgattacca gtgatgattc gctggaagtg attgcccgca
    50881 aactgaatcg cgaaaaagag ctggcgctgg agcaaacctg cgcgttgttc gactacgcgc
    50941 ggctgcaaaa aatcattgag gtcatcagta aagcaccgtt tatccagata acgggcctgg
    51001 gcggatcggc gctggtgggg cgcgatctgt cgttcaaact gatgaaaatt ggatatcgcg
    51061 tggcctgcga agccgacacg cacgtacaag ccaccgtttc ccaggcgttg aaaaaaggcg
    51121 atgtacaaat cgctatctct tacagcggca gtaaaaaaga gattgtcttg tgcgcagaag
    51181 cggcacgaaa gcagggggca acggtgattg ccattacttc tctggctgac tctccgctgc
    51241 ggcggctggc gcatttcacg ctcgataccg tttccggtga aaccgaatgg cgtagctcgt
    51301 cgatgtctac ccgcacggcg caaaattctg ttaccgattt actgtttgtt ggtcttgtgc
    51361 aactcaatga tgtggagtca ttgaaaatga ttcagcgcag cagtgagcta acacaacgcc
    51421 tgaaatgatg cataaagcag cgactggatt gagattttcc tgaattagtg agctgatccg
    51481 cagcaatatt ttgtttatcc tgtattttca gagggaatgg agtgtaacgc tctgtattaa
    51541 caaggagagc attaaaatgg gtaaactcac gggcaagaca gcactgatta cgggcgcatt
    51601 gcagggaatt ggcgaaggaa ttgccagaac ttttgcgcgt catggcgcaa acctaatctt
    51661 gctggatatc tcccctgaga tcgaaaagct ggcggacgaa ctgtgtggtc gtggtcatcg
    51721 ctgtacggcg gttgtcgccg atgtgcgtga cccggcgtcg gtagccgcag ctatcaaacg
    51781 cgcgaaggaa aaagaagggc gcattgatat cctggtgaat aacgcaggcg tttgtcgtct
    51841 gggcagtttc ctcgatatga gcgatgacga tcgcgatttc catattgaca tcaatattaa
    51901 aggcgtatgg aacgtcacga aggcggtgct gccggagatg attgcgcgca aagatggtcg
    51961 cattgtgatg atgtcttcgg tcactggcga tatggtggcc gatccgggtg aaacggcgta
    52021 cgccttaacg aaagcggcga ttgttggcct gactaaatcg ctggcggtgg agtacgcgca
    52081 atccggtatt cgcgttaacg ccatctgccc gggatacgtc cgcacgccaa tggcggaaag
    52141 cattgcccgc cagtcgaacc cggaagatcc agagtcggtg ctgactgata tggcaaaagc
    52201 aatcccgctg cgtcgcctcg ccgatccgct ggaagtcggc gaactggcgg ccttcctcgc
    52261 atcggatgaa tccagctatt taaccggtac acagaatgtg attgatggcg gcagcacact
    52321 gccggagacg gtcagcgtcg gtatctgatt cacctctgtt tcctccctgc atgtgtgggg
    52381 aggatttcgt cttattccct tttcaacttc caaatcacca aacggtatat aaaaccgtta
    52441 ctcctttcac gtccgttata aatatgatgg ctattagaaa gtcattaaat ttataagggt
    52501 gcgcaatggc cgttaactta ctgaaaaaga actcactcgc gctggtcgct tctctgctgc
    52561 tggcgggcca tgtacaggca acggaactgc tgaacagttc ttatgacgtc tcccgcgagc
    52621 tgtttgccgc cctgaacccg ccgtttgagc aacaatgggc caaagataac ggcggcgaca
    52681 aactgacgat aaaacaatct catgccgggt catcaaaaca ggcgctggcg attttgcagg
    52741 gcttaaaagc cgacgttgtc acttataacc aggtgaccga cgtacaaatc ctgcatgaca
    52801 aaggcaagct gatcccggcg gactggcaaa cgcgcctgcc gaataatagc tcgccgttct
    52861 actccaccat gggcttcctg gtgcgtaagg gtaacccgaa gaatatccac gactggaacg
    52921 acctggtgcg ctccgacgtg aagctgattt ttccgaaccc gaaaacgtct ggtaacgcgc
    52981 gttacaccta ccttgcggca tggggcgcag cggacaaagc tgacggtggc gacaaagcca
    53041 aaaccgaaca gtttatgact cagttcctga aaaacgttga agtgttcgat accggcggtc
    53101 gtggcgcgac caccaccttc gccgagcgcg gtatgggcga tgtattgatc agcttcgagt
    53161 cggaagtgaa caacatccgc aaacagtacg aagcgcaggg ctttgaagtg gtgattccga
    53221 aaaccaacat tctggcggaa ttcccggtgg cgtgggtcga taaaaacgtg caggccaacg
    53281 gtacggaaaa agccgcaaaa gcctacctga actggctcta cagcccgcag gcgcagacca
    53341 tcatcaccga ctattactac cgcgtaaata acccggaagt catggacaaa ctgaaagata
    53401 aattcccgca gaccgaactg ttccgtgtgg aagacaaatt tggctcctgg ccggaagtga
    53461 tgaaaaccca cttcaccagc ggcggcgagt tagacaagct gttagcggcg gggcgtaact
    53521 gatgtttgca gtttcatcaa gacgcgtgct gccgggcttt accttaagcc tcggcaccag
    53581 tctgctgttt gtttgcctga ttttgctcct gccactctcc gcgctggtga tgcagctggc
    53641 ccagatgagc tgggcgcagt actgggaggt gatcaccaac ccgcaggtgg tcgcagccta
    53701 caaagtgacg ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt tcggcctgct
    53761 gatggcgtgg atcttaactc gttatcgctt ccctggccgc acgctgcttg atgcgctgat
    53821 ggatttaccc tttgcgctgc caacggcagt cgccggttta acgctggcct cgctcttttc
    53881 cgtgaacggt ttttacggtg aatggctggc gaagtttgat atcaaagtca cctacacctg
    53941 gcttgggatt gcggtggcga tggcctttac cagcattccg tttgtggtac gtaccgtgca
    54001 gccggtgctg gaagagttag gcccggaata tgaagaggcg gcagaaacgc ttggcgcaac
    54061 gcgctggcag agtttctgca aagtggtgct gccggagctt tctccggcgc tggtggcggg
    54121 cgtggcgttg tcgtttaccc gtagcctcgg tgagtttggc gcggtgattt ttatcgccgg
    54181 gaatatcgcg tggaagacgg aagtgacgtc gctgatgatt tttgtgcgct tgcaggagtt
    54241 tgattacccg gcggcgagcg cgattgcctc ggtgatcctc gcggcatctc tgctgctgct
    54301 gttctcaatt aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg
    54361 gaagtgacac agttgaagcg ttatgacgcg cgcccgatta actggggcaa atggtttctg
    54421 attggcaccg ggatgctggt ttcggcgttc atcctgctgg tgccgatggt ttacatcttc
    54481 gtgcaggcat tcagcaaggg gctgatgccg gttttacaga atctggccga tccggacatg
    54541 ctgcacgcca tctggctgac ggtgatgatt gcgctgattg ccgtaccggt aaacctggtg
    54601 ttcggcattc tgctggcgtg gctggtgacg cgatttaact ttcccggacg ccagttactg
    54661 ctgacactgc tggatattcc gtttgccgta tcgccggtgg ttgccggtct ggtgtatctg
    54721 ctgttctacg gctccaacgg cccgctgggt ggctggctgg atgaacataa tctgcaaatt
    54781 atgttctcct ggccggggat ggtgctggtc accatcttcg tgacgtgtcc gtttgtggtg
    54841 cgcgaactgg tgccggtgat gttaagccag ggcagccagg aagacgaagc ggcgattttg
    54901 cttggcgcgt ctggctggca gatgttccgc cgcgtcacat tgccgaacat ccgctgggcg
    54961 ctgctgtatg gcgtagtgct gaccaacgcc cgcgcgattg gcgagtttgg cgcggtgtcg
    55021 gtggtttccg gctcgattcg tggcgaaaca ctttcgctgc cgttacagat tgaattgctg
    55081 gagcaggact acaacaccgt cggctccttt accgccgccg cgctgttgac gctgatggcg
    55141 attatcaccc tgtttttaaa gagtatgttg cagtggcgcc tggaaaatca ggaaaaacgc
    55201 gcgcagcagg aggaacatca tgagcattga gattgccaat attaagaagt cgtttggtcg
    55261 cacccaggtg ctgaacgata tctcactgga tattccttct ggtcagatgg tcgcgttgct
    55321 ggggccgtct ggttccggga aaaccacgct gctgcgcatt attgccgggc tggagcatca
    55381 aaccagcggg catattcgct tccacggcac cgacgtcagc cgtctgcacg cacgcgatcg
    55441 caaggtcggt ttcgtgttcc agcattacgc gctgttccgc catatgacgg tattcgacaa
    55501 tatcgctttt ggcctgacgg tgctgccgcg tcgcgagcgc ccgaatgccg cggcgatcaa
    55561 agcgaaagtg acaaaattgc tggagatggt gcagcttgat catctggcgg atcgttatcc
    55621 ggcgcagctt tccggcggcc agaaacagcg tgtggcgctg gcgcgtgcgc tggctgtgga
    55681 accgcaaatt ctgctgcttg atgaaccgtt tggcgcgctg gatgcgcagg tgcgtaaaga
    55741 gctgcgtcgc tggctgcgtc aactccatga agaactaaaa ttcaccagcg tttttgtgac
    55801 ccacgatcag gaagaagcga ccgaagtagc tgatcgtgta gtggtgatga gccagggcaa
    55861 tattgaacag gctgacgcgc cggatcaggt atggcgcgaa ccggcgaccc gttttgtgct
    55921 cgaatttatg ggcgaagtga accgcctgca gggaaccatt cgcggcgggc ggttccatgt
    55981 tggcgcgcat cgctggccgc tgggctacac acctgcgtat caggggccgg tggatctctt
    56041 cctgcgccct tgggaagtgg atatcagccg ccgtaccagc ctcgattcgc cgctgccggt
    56101 acaggtactg gaagccagcc cgaaaggtca ctacacccaa ttagtggtgc agccgctggg
    56161 gtggtacaac gaaccgctga cggtcgtgat gcatggcgac aatgccccgc agcgtggcga
    56221 gcgtttattc gttggtctgc aacatgcgcg gctgtataac ggcgacgagc gtatcgaaac
    56281 ccgagatgag gaacttgctc tcgcacaaag cgcctgatag gttgagtgaa tgttaaacgc
    56341 ccggaggcgc ttcccgcgat ccgggctttt taatggcaag gtttgtaacc tgtaggcctg
    56401 ataagacgcg caagcgtcgc atcaggcaac accacgtatg gatagagatc gtgagtacat
    56461 tagaacaaac aataggcaat acgcctctgg tgaagttgca gcgaatgggg ccgaataacg
    56521 gcagtgaagt gtggttaaaa ctggaaggca ataacccggc aggttcggta aaagatcgtg
    56581 cggcgctttc gatgatcgtc gaggcggaaa agcgcgggga aattaaaccg ggtgatgtgt
    56641 taatcgaagc caccagtggt aacaccggca ttgcgctggc aatgattgcc gcgcttaaag
    56701 gctatcgcat gaaattgctg atgcccgaca acatgagcca ggaacgccgt gcggcgatgc
    56761 gtgcttatgg tgcggaactg attctggtca ccaaagagca gggcatggaa ggtgcgcgcg
    56821 atctggcgct ggagatggcg aatcgtggtg aaggaaagct gctcgatcag ttcaacaatc
    56881 ccgataaccc ttacgcccat tacaccacta ccgggccgga aatctggcag cagactggcg
    56941 ggcgcattac ccattttgtc tccagcatgg gaacaaccgg cactatcacc ggcgtgtcac
    57001 gctttatgcg cgaacaatcc aaaccggtga ccattgtcgg cctgcaaccg gaagagggca
    57061 gcagcattcc cggcattcgc cgctggccta cggaatatct gccggggatt ttcaacgctt
    57121 ctctggtgga tgaggtgctg gatattcatc agcgcgatgc ggaaaacacc atgcgcgaac
    57181 tggcggtgcg ggaaggaata ttctgtggcg tcagctccgg cggcgcggtt gccggagcac
    57241 tgcgggtggc aaaagctaac cctggtgcgg tggtggtagc gatcatctgc gatcgtggcg
    57301 atcgctacct ttccaccggg gtgtttgggg aagagcattt tagtcagggg gcggggattt
    57361 aagattattg cgcaggcact gttttatccg tctgcgcaat taacgcatgt aaaaaaggct
    57421 gtgcgttctc atcgctctta ccggggatct tcacaatata cggcttcccg gtaatcgatg
    57481 acgacgaagc aaccttatca ataaactgct cggcagtgtc aatgcggtta cgggtattgc
    57541 ccagcttcag acgcagatga gaaaccgctt catcgcaggt atgttcatca ccgttgcgca
    57601 caaatatcaa atcctttttc tgtgctaatc cctccagcat ggcgttgatg cgggcttctt
    57661 catgggcagt taacttcgcg taagcgggga gtgtcattag tagtgtaatg accagacaaa
    57721 tgattttctt cactgaatta ccttatcctt gttttgtaat agccgatttg actgccagaa
    57781 tcaggtaaag tttcggttgg aatacgatga ggatcatcgg caaggagaaa aaattaaatg
    57841 agtagtttgt tgttgtttaa cgataagagc agggcgctgc aggcggatat cgtcgccgtg
    57901 cagtcgcagg tagtgtacgg cagcgtaggc aacagcattg ccgtgcctgc tatcaaacag
    57961 aacggcctga atgtctttgc cgtgccgacg gtattgctga gcaatacgcc gcattatgac
    58021 actttctacg gtggtgcgat tccggatgaa tggtttagcg gctatttgcg tgcgcttcag
    58081 gagcgtgatg cgctgcgcca acttcgagct gtaaccacgg gctatatggg aacggcatcg
    58141 caaatcaaaa tccttgccga gtggctgact gcgctacgca aagaccatcc tgatctattg
    58201 atcatggtcg atccggtgat tggcgatatt gatagcggaa tttatgtcaa acctgacctt
    58261 cccgaggcgt atcgacaata tttactgccg ctggcgcagg gaattacccc caatatcttt
    58321 gagctggaaa tcctgaccgg taaaaattgc cgcgatctcg acagtgccat tgctgccgca
    58381 aaaagtctgc tttcagacac actaaaatgg gtggtgatta ccagcgcctc cggtaatgaa
    58441 gaaaatcagg agatgcaggt tgtggtggtc actgccgaca gcgtgaatgt catttcccat
    58501 tcaagggtaa aaaccgacct gaaagggact ggcgacctgt tttgtgctca gcttatcagt
    58561 ggtttgctga aagggaaggc attaaacgat gcggtgcacc gcgcggggtt gcgcgtactg
    58621 gaagtgatgc gctacaccca gcagcatgag agcgatgaat tgattttgcc gccgctggcg
    58681 gaagcataaa aaaatggcgc cgatgagcgc catttttcac tgcggcaaga attacttctt
    58741 gatgcggata accggggttt cacccacagt cacgctaccg gacagtttga tcagttcttt
    58801 gatttcgtcc atgttggaga taacaaccgg agtcagggta gacttggctt tctcttccag
    58861 cagcggcaga tcaaattcaa tgacagtatc gccaactttc acgcgctgac cttcttcagc
    58921 aatacgcttg aagccttcgc ctttcagttc aacggtgtcg ataccgaagt ggacgaacag
    58981 ttcaacgccg ctatcagatt cgatagagaa tgcgtggttg gtttcaaaga ttttaccaat
    59041 ggtgccgtct acaggcgcga ccattttgtt acccgttggt ttgatagcaa taccatcacc
    59101 aacgattttt tccgcaaaaa cgacatccgg cacgtcttcg atattgacga tctcgccaga
    59161 gagcggagca atgatctcaa tagttccggt atccttcttg tcgtcggaaa ccagagattt
    59221 cagtttatcg aacaaaccca tgatcttctc ctaagcagta aattgggccg catctcgtgg
    59281 attagcagat tgttttttct tcaatgaact tgttaaccag cgtcattaac tcgtccgttg
    59341 tcggttgagc aagagcctgc tctgctaaca ccttcgcatc ttcgaagttc gtgttacgga
    59401 taatcttctt aatgcgcggg atagaaatgg cgctcataga gaattcgtcc agacccatcc
    59461 ccagcaacag aagtgtagca cgttcatcgc cagcaagctc accacacatg ccagtccatt
    59521 tgccttcagc atgagaagca tcaataactt gcttgatcaa gttcagcacg gacggtgaca
    59581 ttggctggta aaggtgtgaa atcatatcat taccacggtc aactgccaga gtgtactgcg
    59641 ttaaatcatt ggtgccgata ctaaagaaat caacttcttt ggctaaatga cgtgcaattg
    59701 ttgcggcagc cggtgtttcc accattacgc cgatttcaat tgactcgtca aacgctttac
    59761 cttcgtcgcg cagttcctgt ttgtagattt cgatctcttt gcgcagtgca cgcacttctt
    59821 caacagagat gatcatcggg aacataatgc gcaatttacc gaaagccgag gcacgcagga
    59881 tagcgcggag ctgatcgcgc aggatctctt tacgatccat cgcgatacgg atagcgcgcc
    59941 agccgaggaa cgggttctct tctttcggga agttcatgta tggcagctct ttgtcgccgc
    60001 cgatgtccat ggtacgaacg ataaccgctt gcgagccaca cgcttcagcc actgctttgt
    60061 aagcagcaaa ctgttcttct tcagtgggca gcgcgtcgcg gtccatgaac aggaactcag
    60121 tacgatacag accaacgcct tcagcgccgt tacgctctgc accttcaacg tcacgaaccg
    60181 taccaatgtt agcgcatact tctacctggt gaccgtccag cgtaatagct ggcagatctt
    60241 tcagtttagc aagctctgct ttttcagaag ccacttgctc ctgaacagcg cgcattttat
    60301 caataacttc gttggttgga ttgacgtaaa cctgattatt tacggcatcc agaatcagat
    60361 agtcgtcatt tttcacctga gaggtgacgc taccggtacc cacgatagca ggcagttcca
    60421 gagaacgcgc catgatagag gtgtgggaag tacggccacc cgcgtcggtg atgaaaccca
    60481 gcaccttctt caggttcagc tgtgcggttt cggacggcgt caggtcagcg gcaaccagaa
    60541 tgacttcatc ctgaatggcg ctcaggtcga taatcttcag gcccaggatg ttgcgcagca
    60601 ggcgcttacc gatatcacgt acgtcagccg cacgttcttt caggtattca tcatccagct
    60661 cttccagggc agaagcctga ccttcgataa cttcatgagc agctgcgtca gctgtcatgt
    60721 gtttatcttt aatcagggct atgatttcct gctccagctc ctcatcttcg agcagcataa
    60781 tatgcccttc aaagatggct tctttttctt caccgaacgt ttcaccagct ttcgttttga
    60841 tcgtttccag ctgggctgat gccttggcac gaccgctcag aaaacgttca acttcctgat
    60901 caacctggtc ggcagaaatt tttttccggt caatgacaat ttcgtcttct ttcagaagca
    60961 gagctttacc gaaagcgata cccggggatg ctaaaatgcc tgaaatcata accctacctt
    61021 acttgtgact gatttttaaa agaacccggg aaattactcg agttccgcca tcagtttaac
    61081 cagatgttca accgctttct gctcgtcttc gccttctgcg gagatagtca caacggtacc
    61141 ttgagtcagg cccagagtct gcagtttaaa caggcttttc gcgctggcgc ttttgccgtt
    61201 ggaagtcaca gtaatttcag aagtgaagcc cttagcttct tttacaaact gggcagcagg
    61261 gcgggtgtgc agaccgttcg gagcggtaat ggtaacttct tgctggaaca ttgtatttcc
    61321 ccaacttata ggtttagtgt tgtggaacta aagtctagcc tggcggttcg actttagcct
    61381 gtattgttag cgccagtttt taacagacgc gacgcacgaa gagcgcaata cagtccattg
    61441 ctggcgggtg ttgaccgctg acgtttcgta cgtcattaaa cattatgccg ctaaaggaag
    61501 aattgaacca aatcataaaa tcgattcagc tttggctttt cctgtaacga ttaatttcgc
    61561 gcatcaaaat aattagtctg gttaaatacc agttccgaca ggctgaatca atgccaggag
    61621 gggcgaaagt ttgaagcagg ccacaaaaaa gcacctaaaa aggtgctttt ttacgcattt
    61681 ttaacaagct ggcattactg ttgcaattct ttctcagtga agagatcggc aaacaatgcg
    61741 gtgcttaaat aacgctcacc cgatgacggt agaataacca caatattctt gttggtaaag
    61801 ctttcatctt cttgtagttt caacgccgca gcaacagctg ctccagaaga gatacctgca
    61861 agaatacctt cttcttccat cagacgacgg gcggtagaaa tcgcttcttc attggtgatg
    61921 ccaatgactt tatcgaccag cttgagatcg aggttagccg ggataaaacc agcgccaata
    61981 ccctgaattt tatgcgggcc aggtttaatc tcttcacctg ccagcgcctg ggcgataact
    62041 ggagaatcgg ttggctcaac ggcgacagag ataagatcgg tcttaccttt ggtgccttta
    62101 atgtagcggc tgacgccagt cagcgtaccg ccagtcccaa cgccagcaat aaatacatca
    62161 acctgaccgt cggtatcttc ccagatctcc ggaccggtgg tcttttcgtg aatttcaggg
    62221 tttgccggat tgctgaattg ttgcagcagc aggtatttct ctggattgct ggcgacaatt
    62281 tcttctgctt tttggattgc gcctttcatg cctttagcac cttcggtcag caccaggttt
    62341 gcacctaacg ctttcagcag cttgcggcgt tcaatactca tggtttctgg catggtcagg
    62401 gtgagtttgt aaccgcgagc ggcagctaca taggccagtg caatcccagt attaccgctg
    62461 gttggttcaa ccagttcaac gcctggtttc agcacgccgc gcttttcggc atcccaaatc
    62521 atgttggcac cgatacggca cttaacgctg aagctggggt tacgagattc caccttcgcc
    62581 agaatgcgtc cgttaccgat gcgattcagg cgaaccagcg gcgtgtgacc gatagtcagc
    62641 gagttatctt caaaaatctt actcatggcc tgtccttaac tgtatgaaat tgggatacaa
    62701 caggtagcat acccgctcag agaatatgcg gaagtaagga tttagcatat ctatatacag
    62761 aagggaaata atgacatgca agatggaata aggggcgaca taagccaccc ctgtttcaca
    62821 caaacggttt acaaaataac cggtagattg ttaccgccac atcgcgtgtt tatcgcgata
    62881 gcaatcgacc cacatcgccg tcgcgccaca aacggcaacg ggcatgatga acagattaag
    62941 cagcgggatc atcgtaaaca ggctggttaa agcaccaaac tgcatattgg tgattttgcg
    63001 tgtgcgcagg gcggtgcgca tctctttaaa cggcactttg tggttatcga aggggtaatc
    63061 gcaatactgg atggctaaca tccaggcgct aaacaggaac cacagtaccg gcgcgacggt
    63121 ttgcccaata ccagggatga agtaaagaat tagtaataca attgcgcgcg gcagatacca
    63181 agcaaatttt tgccattcgc gtttcatgat tcgcggcaca tctttcatga taccgaaaat
    63241 cccggtatct ggcggtgtag cgccagtcaa tcgtgcttcc agttgttcag ccaataaacc
    63301 gttaaacgga gcggcaatcc agttagcaat cgtggagaag aaatagccaa acactaacag
    63361 cacagagatg acagccagag gccacaacag atagctcagc cattgtaacc agtccggaac
    63421 ataactcatg agagtcggga tccagacatc gagttgcgta aagagccacc agaatgcgcc
    63481 ccccatcaac aaaatattga ccagcagcgg taaaataacg aaacgccgaa tcccaggttg
    63541 cgagacgagc ttccagcctt gcgcaaaata gtaaaaaccg ctgcgtgggg cagatgtgaa
    63601 tgatgaaacc ataatcagga tgagctcctt ttgaccaatc ccaggaaaat tgtgcgtatt
    63661 ttaccgggta attgcgcaat ggacagttag gatatgttcg aaaaaacagc aaaaagcacg
    63721 atttcatcta tctttgtgct gtgaaagtta atagtgcact tgcacttgag gtaatcggca
    63781 aatactctta gtgagtaaat gtttgccgtg gtggcaaggt gttagaacaa cagagaatat
    63841 aatgatgcag gatttgcgtc tgatattaat cattgttggc gcgatcgcca taatcgcttt
    63901 actggtacat ggtttctgga ccagccgtaa agaacgatct tctatgttcc gcgatcggcc
    63961 attaaaacga atgaagtcaa aacgtgacga cgattcttat gacgaggatg tcgaagatga
    64021 tgagggcgtt ggtgaggttc gtgttcaccg cgtgaatcat gccccggcta acgcccagga
    64081 gcatgaggct gctcgtccgt cgccgcaaca ccagtaccaa ccgccttatg cgtctgcgca
    64141 gccgcgtcaa ccggtccagc agccgcctga agcgcaggta ccgccgcaac atgctccgcg
    64201 tccagcgcag ccggtgcagc aacccgtgca gcagccggcc tatcagccgc agcctgaaca
    64261 gccgttgcag cagccagttt tgccacaggt cgcgccatcg ccgcagccag tgcattcagc
    64321 accgcaaccg gcacaacagg ctttccagcc tgcagaaccc gtagcggcac cacagcctga
    64381 gcctgtagcg gaaccggctc cagttatgga taaaccgaag cgcaaagaag cggtgattat
    64441 catgaacgtc gcggcgcatc acggtagcga gctaaacggt gaactgcttc ttaacagcat
    64501 tcaacaagcg ggcttcattt ttggcgatat gaatatttac catcgtcatc ttagcccgga
    64561 tggcagcggc ccggcgttat tcagcctggc gaatatggtg aaaccgggaa cctttgatcc
    64621 tgaaatgaag gatttcacta ctccgggtgt cactatcttt atgcaggtac cgtcttacgg
    64681 tgacgagctg cagaacttca agctgatgct gcaatctgcg cagcatattg ccgatgaagt
    64741 gggcggtgtc gtgcttgacg atcagcgccg tatgatgact ccgcagaaat tgcgtgagta
    64801 ccaggacatc atccgcgaag ttaaagacgc caacgcctga tacacttaag gcaaattaac
    64861 tcctcttcga acccccgctt gtcgggggtt tttagcattg atggtgcgat atggaatcaa
    64921 tcgaacaaca actgacagaa ctgcgaacga cgcttcgcca tcatgaatat ctttatcatg
    64981 tgatggatgc gccggaaatt cccgacgctg aatacgacag gctgatgcgc gaactgcgcg
    65041 agctggaaac caaacatcca gaactgatta cgcctgattc gcctacccaa cgtgtaggtg
    65101 ctgcgccgct ggcggctttc agccagattc gccatgaagt gccaatgctg tcgctggata
    65161 acgtttttga tgaagaaagc tttcttgcct tcaacaaacg agtgcaggac cgtctgaaaa
    65221 gcaacgagaa agtcacctgg tgctgtgagc tgaagctgga tggccttgct gtcagtattc
    65281 tgtatgaaaa tggcgtttta gtcagtgccg cgacccgtgg cgatggcacc accggggaag
    65341 atatcacgtc taacgtgcgt accattcgcg ccattccact gaagctgcac ggagagaata
    65401 tcccggcgcg tctggaagtg cgtggtgaag tgttcctgcc gcaggcgggg ttcgaaaaga
    65461 ttaacgaaga tgcgcgacgc acgggcggga aagtttttgc taacccacgt aatgcggcag
    65521 ctggttcact gcgtcagctt gatccgcgta ttacagcgaa gcgaccgctc acttttttct
    65581 gctatggcgt tggtgttctg gaaggtggcg agctgccgga tactcatctt ggccgtttac
    65641 tgcaatttaa aaagtggggg ttgccggtca gcgatcgggt aacgctttgt gaatcggcgg
    65701 aagaagtgct ggcgttctat cacaaagtgg aagaagaccg cccgacgctg ggctttgata
    65761 tcgacggcgt ggtgattaag gtcaactcac tggcacagca ggagcagctt ggctttgtcg
    65821 cgcgagcccc gcgctgggcg gtagcgttta aattcccggc gcaggaacag atgacctttg
    65881 tgcgtgacgt cgagtttcag gttgggcgta ctggcgcgat tacgcctgtt gcgcgtctgg
    65941 aacctgtcca tgttgcaggc gtgctggtga gtaacgcaac cttacacaat gcggatgaaa
    66001 tcgaacgtct tggtttacgc attggcgata aagtggtgat tcgccgcgct ggcgacgtga
    66061 tcccgcaggt ggttaacgtt gtgctttctg aacgcccgga agatacccgt gaggttgtat
    66121 tcccgacgca ttgtccggta tgtggttctg acgttgagcg tgtggaaggt gaagcggttg
    66181 cccgctgtac cggtggcctg atttgcggtg cgcagcgtaa agagtcgctg aaacactttg
    66241 tttcccgccg tgcgatggat gttgacggaa tgggcgacaa aatcatcgat cagctggttg
    66301 aaaaagaata tgtccacact ccggcggatc tgttcaaact caccgcaggc aaacttaccg
    66361 gactggagcg tatggggcca aaatcggcac aaaacgtggt taacgcgctg gaaaaagcga
    66421 aagaaaccac ctttgctcgc ttcctctatg cacttggcat ccgtgaagtc ggcgaggcca
    66481 ccgcagcagg tctggcggca tatttcggca cgctggaagc gctggaagcc gcttcgattg
    66541 aagagctgca aaaggtgcct gatgttggca ttgtcgttgc atcccacgtt cacaacttct
    66601 ttgccgaaga aagcaaccgc aatgtcatca gcgagctgtt ggcggaaggt gttcactggc
    66661 ctgcgccgat cgttatcaac gcggaagaga ttgacagccc gtttgctggt aaaaccgtgg
    66721 tgcttacggg cagcttaagc cagatgtcgc gtgatgacgc taaagctcga ctggtcgaac
    66781 tgggcgcgaa agtcgcgggc agcgtgtcga agaaaaccga tctggtgata gcgggtgaag
    66841 ctgcaggatc taaactggcg aaggcgcagg aactgggcat tgaagtcatc gacgaagcgg
    66901 aaatgctgcg tttgctgggt agctgagatg gaaaaagagc agctgattga aatagccaat
    66961 acgataatgc cgtttggcaa atacaaaggg cgtcgcttaa tcgacctgcc ggaggagtat
    67021 ctgctgtggt ttgcccgcaa agatgagttc ccggcaggga agctcggtga gctaatgcaa
    67081 atcacgctgc tgattaaaac cgaggggctg acgcaactgg tccagcccct gaagcgtccg
    67141 ctttaagctt tatcggtgct gctttcctgc tgcgcctgta actgttcggt ctggcgtttg
    67201 tatcgacgcg ccagcaccgc acagaccatc aattggatct gatggaaaat catcaggggc
    67261 agcaccatca taccgatcac cgatgtgggg aacagaatgt ttgccatcgg gatgccattt
    67321 gccagactct ttttcgaacc acaaaagacg atagtaattt catctgcctt attgaagccc
    67381 agtcggcgtg ccataaagac gttaactaca atcacgatag ccagaagaac gcagctgacc
    67441 accacgataa acagcaatga tccccagcca actttatgcc agataccatt aacgacggct
    67501 tcgctgaacg ctgtataaac caccaacaga atggacgtct ggtcagtttt cgcaatccat
    67561 tttttattgc gcgacaccca gtctccaatc cacggccggg aaagatgccc caacacaaac
    67621 ggcagcagca gttgcagcat aattttaccg acctgctcaa ggctgccccc tgcaccgtga
    67681 acattcatca ccagaccaac cagcaatggt gaaaggaaaa tccccagcag gctggatgct
    67741 gacgcagaac aaaccgccgc cgcgacgtta ccgcccgcca ttgacgtgaa ggcgattgca
    67801 gactgcacgg tagccgggag aatgcacaag tagagaaaac cggagtagag catcgggtcg
    67861 acatttaccg gtttccacca ggcaaacagt acacccagaa tcggaaacag cacgaaggtg
    67921 ctgcacatta cccacaaatg cagtcgccag tgaccaccgc cagcaataat cgcctcacgc
    67981 gacaacttcg cgccgtgcat aaagaacagc agggcaatag ctgcggtggt cagattttca
    68041 aagaagggga cgaaatcgcc tctggccgga aagaaagagg ccagcaacac caccgtgatc
    68101 agggttaagg tgaaaggatc gaggatacga aaaagtttca taaagactcc agatgatcga
    68161 tggttctatt gtgcttcttc cagtttgaga aataaaattg atttattgca tccattcatg
    68221 aagaaaacag atgaattatt ctttaaaaca attaaaggtt ttcgtcacag tagcgcagga
    68281 gaaaagtttt agtcgtgcag gagagcgtat cggcctgagc cagtcggcag tgagtcacag
    68341 tgtgaaggag ctggaaaatc atactggtgt tcgcctgctg gacagaacca cgcgtgaagt
    68401 ggtgcttaca gatgcagggc agcagttggc tttgcgtctt gagcgactgc tggatgaact
    68461 taacagcacg ttgcgcgata ccgggcgtat ggggcaacaa ctgagcggaa aagttcgggt
    68521 cgctgccagc cagaccattt ccgcgcatct tattccgcaa tgcattgctg aaagccatcg
    68581 ccgctatcca gatattcagt ttgtcctgca cgatcgtccg cagcagtggg tgatggaaag
    68641 tattcgccag ggagatgtcg actttggcat cgtcatcgat ccaggcccag tgggcgatct
    68701 gcaatgtgaa gcgattcttt ccgagccttt ctttctgctt tgccatcgcg atagcgcttt
    68761 ggccgtggaa gattatgtac cctggcaggc attacaggga gcgaaattgg tgttgcagga
    68821 ttacgcgtca ggcagccgac cgctgattga cgcagcgctg gcgcgcaatg gtattcaggc
    68881 gaatattgtg caggagattg gacatccggc gacgctgttc ccgatggtag ccgcaggcat
    68941 cggcatcagt attcttcccg cactggcgct accattgcct gaaggtagcc cgctggtggt
    69001 aaagcgcatt acgccggttg ttgaacgcca gttgatgctg gtgcgcagga agaaccgttc
    69061 actttccacc gccgctgaag cgttatggga cgtggtacga gaccagggca atgcgctaat
    69121 ggctggtcgg gaaggggatc cgttgtatca gatatagata tcgatatgag tcgtattttc
    69181 aacaggattt agaagggata cattcagaga caaacgctgc tctttctcct gcgccttttc
    69241 tgcctgctgt ctctgcaact gcgccagttg tgtttccagt agggtgattt gctgttgaat
    69301 caattcggcc tgtttttgct tgtcttcagc actgccgcta gtgtctacaa tctctttaat
    69361 ttgttgagtg agtttgatga tttgctgact tatccgcgcg atttgtgaag caacatcgct
    69421 accttgtgat gccctgttac tactctgtgt ttgtatggaa ggaattgaag ttgaagagat
    69481 agtggccatt gttcagtcct cgtcacttta cgaggtaata tcggtagaag aacaaggaac
    69541 ttgagaagaa aaatcaatct tacaaatcgc a
//
LOCUS       DAZFFG010000010        67603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFFG010000010 DAZFFG010000000
VERSION     DAZFFG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.010"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..246
                     /locus_tag="ACHJ7J_000903"
     CDS             <1..246
                     /locus_tag="ACHJ7J_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFO4999174.1"
                     /translation="SRKDHFDIISNQCPVLIPLYKHRYMSTSSYTGGNPVYSVYNSDI
                     ICIGNDLTCWMETEFNLALSGHYQADKKPVKFWDDFL"
     gene            complement(241..957)
                     /locus_tag="ACHJ7J_000904"
     CDS             complement(241..957)
                     /locus_tag="ACHJ7J_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999175.1"
                     /translation="MEWLSEIRKLRESISVGIQAARRLLEKTGGDVDEAIKLFHIDQI
                     NILTAKVDVTHQEAENVLLAANYDMAEALRRIDEQRYTLTELIIRKNKDAGDALSNIE
                     LAITYEWNLTCKFWFGFKAFQSLPPQLQAFMLVCEWRNYWGWEGLDSALYYEMDNVPQ
                     QLQVVGLDEMAKVIVVARNRYDELRAQGKEHNKIIKDDKFKKFMKHCEQLDSEVDSIL
                     LQFVKDNIEVFPRRHNGHDL"
     gene            1140..1562
                     /locus_tag="ACHJ7J_000905"
     CDS             1140..1562
                     /locus_tag="ACHJ7J_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999176.1"
                     /translation="MTPLERITQLVNINGDVNDPDTLRPLLSLEDFFIGNNIVGSICC
                     NVTPEQSPQTIYHHLQKIRERNDVSDVLVEITMFDDPDWPFSERILVITSASPEEVQS
                     WFVEEIAPDECWEGWSEDTNHGWVEVPAGMHPVICWWD"
     gene            complement(1640..3097)
                     /gene="nuoN"
                     /locus_tag="ACHJ7J_000906"
     CDS             complement(1640..3097)
                     /gene="nuoN"
                     /locus_tag="ACHJ7J_000906"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO4999177.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3104..4633)
                     /gene="nuoM"
                     /locus_tag="ACHJ7J_000907"
     CDS             complement(3104..4633)
                     /gene="nuoM"
                     /locus_tag="ACHJ7J_000907"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO4999178.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4797..6638)
                     /gene="nuoL"
                     /locus_tag="ACHJ7J_000908"
     CDS             complement(4797..6638)
                     /gene="nuoL"
                     /locus_tag="ACHJ7J_000908"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO4999179.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6635..6937)
                     /gene="nuoK"
                     /locus_tag="ACHJ7J_000909"
     CDS             complement(6635..6937)
                     /gene="nuoK"
                     /locus_tag="ACHJ7J_000909"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO4999180.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6934..7488)
                     /gene="nuoJ"
                     /locus_tag="ACHJ7J_000910"
     CDS             complement(6934..7488)
                     /gene="nuoJ"
                     /locus_tag="ACHJ7J_000910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO4999181.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7500..8042)
                     /gene="nuoI"
                     /locus_tag="ACHJ7J_000911"
     CDS             complement(7500..8042)
                     /gene="nuoI"
                     /locus_tag="ACHJ7J_000911"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO4999182.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8057..9034)
                     /gene="nuoH"
                     /locus_tag="ACHJ7J_000912"
     CDS             complement(8057..9034)
                     /gene="nuoH"
                     /locus_tag="ACHJ7J_000912"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO4999183.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9031..11757)
                     /gene="nuoG"
                     /locus_tag="ACHJ7J_000913"
     CDS             complement(9031..11757)
                     /gene="nuoG"
                     /locus_tag="ACHJ7J_000913"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO4999184.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFKVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11810..13147)
                     /gene="nuoF"
                     /locus_tag="ACHJ7J_000914"
     CDS             complement(11810..13147)
                     /gene="nuoF"
                     /locus_tag="ACHJ7J_000914"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO4999185.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13144..13644)
                     /gene="nuoE"
                     /locus_tag="ACHJ7J_000915"
     CDS             complement(13144..13644)
                     /gene="nuoE"
                     /locus_tag="ACHJ7J_000915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO4999186.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13647..15449)
                     /gene="nuoC"
                     /locus_tag="ACHJ7J_000916"
     CDS             complement(13647..15449)
                     /gene="nuoC"
                     /locus_tag="ACHJ7J_000916"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO4999187.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15543..16205)
                     /gene="nuoB"
                     /locus_tag="ACHJ7J_000917"
     CDS             complement(15543..16205)
                     /gene="nuoB"
                     /locus_tag="ACHJ7J_000917"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO4999188.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16221..16664)
                     /gene="nuoA"
                     /locus_tag="ACHJ7J_000918"
     CDS             complement(16221..16664)
                     /gene="nuoA"
                     /locus_tag="ACHJ7J_000918"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO4999189.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17295..18233)
                     /gene="lrhA"
                     /locus_tag="ACHJ7J_000919"
     CDS             complement(17295..18233)
                     /gene="lrhA"
                     /locus_tag="ACHJ7J_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO4999190.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
     gene            19153..20370
                     /gene="alaA"
                     /locus_tag="ACHJ7J_000920"
     CDS             19153..20370
                     /gene="alaA"
                     /locus_tag="ACHJ7J_000920"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO4999191.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGIRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20454..21053
                     /gene="yfbR"
                     /locus_tag="ACHJ7J_000921"
     CDS             20454..21053
                     /gene="yfbR"
                     /locus_tag="ACHJ7J_000921"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4999192.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21112..22944)
                     /gene="yfbS"
                     /locus_tag="ACHJ7J_000922"
     CDS             complement(21112..22944)
                     /gene="yfbS"
                     /locus_tag="ACHJ7J_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO4999193.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23031..23681)
                     /gene="hxpA"
                     /locus_tag="ACHJ7J_000923"
     CDS             complement(23031..23681)
                     /gene="hxpA"
                     /locus_tag="ACHJ7J_000923"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO4999194.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23692..24186)
                     /gene="yfbU"
                     /locus_tag="ACHJ7J_000924"
     CDS             complement(23692..24186)
                     /gene="yfbU"
                     /locus_tag="ACHJ7J_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO4999195.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24269..24724)
                     /gene="yfbV"
                     /locus_tag="ACHJ7J_000925"
     CDS             complement(24269..24724)
                     /gene="yfbV"
                     /locus_tag="ACHJ7J_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO4999196.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25062..26264
                     /gene="ackA"
                     /locus_tag="ACHJ7J_000926"
     CDS             25062..26264
                     /gene="ackA"
                     /locus_tag="ACHJ7J_000926"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO4999197.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26339..28483
                     /gene="pta"
                     /locus_tag="ACHJ7J_000927"
     CDS             26339..28483
                     /gene="pta"
                     /locus_tag="ACHJ7J_000927"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4999198.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28673..30193
                     /gene="yfcC"
                     /locus_tag="ACHJ7J_000928"
     CDS             28673..30193
                     /gene="yfcC"
                     /locus_tag="ACHJ7J_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO4999199.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30226..30768)
                     /gene="yfcD"
                     /locus_tag="ACHJ7J_000929"
     CDS             complement(30226..30768)
                     /gene="yfcD"
                     /locus_tag="ACHJ7J_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO4999200.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30826..31377)
                     /gene="yfcE"
                     /locus_tag="ACHJ7J_000930"
     CDS             complement(30826..31377)
                     /gene="yfcE"
                     /locus_tag="ACHJ7J_000930"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO4999201.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31433..32077)
                     /gene="yfcF"
                     /locus_tag="ACHJ7J_000931"
     CDS             complement(31433..32077)
                     /gene="yfcF"
                     /locus_tag="ACHJ7J_000931"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO4999202.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVRHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32213..32860
                     /gene="yfcG"
                     /locus_tag="ACHJ7J_000932"
     CDS             32213..32860
                     /gene="yfcG"
                     /locus_tag="ACHJ7J_000932"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO4999203.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32917..33279
                     /gene="folX"
                     /locus_tag="ACHJ7J_000933"
     CDS             32917..33279
                     /gene="folX"
                     /locus_tag="ACHJ7J_000933"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO4999204.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33300..34193
                     /gene="yfcH"
                     /locus_tag="ACHJ7J_000934"
     CDS             33300..34193
                     /gene="yfcH"
                     /locus_tag="ACHJ7J_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO4999205.1"
                     /translation="MNIVITGGTGLIGRHLIPRLQELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34241..35131)
                     /gene="rpnB"
                     /locus_tag="ACHJ7J_000935"
     CDS             complement(34241..35131)
                     /gene="rpnB"
                     /locus_tag="ACHJ7J_000935"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO4999206.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRIMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(35328..36101)
                     /gene="hisP"
                     /locus_tag="ACHJ7J_000936"
     CDS             complement(35328..36101)
                     /gene="hisP"
                     /locus_tag="ACHJ7J_000936"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO4999207.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSTRLQQFLKGSLK"
     gene            complement(36109..36825)
                     /gene="hisM"
                     /locus_tag="ACHJ7J_000937"
     CDS             complement(36109..36825)
                     /gene="hisM"
                     /locus_tag="ACHJ7J_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4999208.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36822..37508)
                     /gene="hisQ"
                     /locus_tag="ACHJ7J_000938"
     CDS             complement(36822..37508)
                     /gene="hisQ"
                     /locus_tag="ACHJ7J_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO4999209.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37598..38380)
                     /gene="hisJ"
                     /locus_tag="ACHJ7J_000939"
     CDS             complement(37598..38380)
                     /gene="hisJ"
                     /locus_tag="ACHJ7J_000939"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO4999210.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGADPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38601..39383)
                     /gene="argT"
                     /locus_tag="ACHJ7J_000940"
     CDS             complement(38601..39383)
                     /gene="argT"
                     /locus_tag="ACHJ7J_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO4999211.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDTELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39649..40218)
                     /gene="ubiX"
                     /locus_tag="ACHJ7J_000941"
     CDS             complement(39649..40218)
                     /gene="ubiX"
                     /locus_tag="ACHJ7J_000941"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO4999212.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHNARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40313..41830)
                     /gene="purF"
                     /locus_tag="ACHJ7J_000942"
     CDS             complement(40313..41830)
                     /gene="purF"
                     /locus_tag="ACHJ7J_000942"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO4999213.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41867..42355)
                     /gene="cvpA"
                     /locus_tag="ACHJ7J_000943"
     CDS             complement(41867..42355)
                     /gene="cvpA"
                     /locus_tag="ACHJ7J_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO4999214.1"
                     /translation="MVWIDYAIIVVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42888..43550)
                     /gene="dedD"
                     /locus_tag="ACHJ7J_000944"
     CDS             complement(42888..43550)
                     /gene="dedD"
                     /locus_tag="ACHJ7J_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO4999215.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43540..44808)
                     /gene="folC"
                     /locus_tag="ACHJ7J_000945"
     CDS             complement(43540..44808)
                     /gene="folC"
                     /locus_tag="ACHJ7J_000945"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO4999216.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGKELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44878..45792)
                     /gene="accD"
                     /locus_tag="ACHJ7J_000946"
     CDS             complement(44878..45792)
                     /gene="accD"
                     /locus_tag="ACHJ7J_000946"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO4999217.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45948..46607)
                     /gene="dedA"
                     /locus_tag="ACHJ7J_000947"
     CDS             complement(45948..46607)
                     /gene="dedA"
                     /locus_tag="ACHJ7J_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4999218.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46690..47502)
                     /gene="truA"
                     /locus_tag="ACHJ7J_000948"
     CDS             complement(46690..47502)
                     /gene="truA"
                     /locus_tag="ACHJ7J_000948"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO4999219.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47502..48515)
                     /gene="usg"
                     /locus_tag="ACHJ7J_000949"
     CDS             complement(47502..48515)
                     /gene="usg"
                     /locus_tag="ACHJ7J_000949"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4999220.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48581..49717)
                     /gene="pdxB"
                     /locus_tag="ACHJ7J_000950"
     CDS             complement(48581..49717)
                     /gene="pdxB"
                     /locus_tag="ACHJ7J_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO4999221.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49816..50811
                     /gene="flk"
                     /locus_tag="ACHJ7J_000951"
     CDS             49816..50811
                     /gene="flk"
                     /locus_tag="ACHJ7J_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO4999222.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSTLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50808..51986)
                     /gene="yfcJ"
                     /locus_tag="ACHJ7J_000952"
     CDS             complement(50808..51986)
                     /gene="yfcJ"
                     /locus_tag="ACHJ7J_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HFO4999223.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52270..53490)
                     /gene="fabB"
                     /locus_tag="ACHJ7J_000953"
     CDS             complement(52270..53490)
                     /gene="fabB"
                     /locus_tag="ACHJ7J_000953"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO4999224.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53649..55655
                     /gene="mnmC"
                     /locus_tag="ACHJ7J_000954"
     CDS             53649..55655
                     /gene="mnmC"
                     /locus_tag="ACHJ7J_000954"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO4999225.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRSDLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSHKDDCWLLNFAGDK
                     QATHSVVVLANGHQISRFSQTSSLPIYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDDQQLNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55776..56054)
                     /gene="yfcL"
                     /locus_tag="ACHJ7J_000955"
     CDS             complement(55776..56054)
                     /gene="yfcL"
                     /locus_tag="ACHJ7J_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO4999226.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(56088..56636)
                     /gene="epmC"
                     /locus_tag="ACHJ7J_000956"
     CDS             complement(56088..56636)
                     /gene="epmC"
                     /locus_tag="ACHJ7J_000956"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO4999227.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEELN"
     gene            complement(56636..57445)
                     /gene="yfcA"
                     /locus_tag="ACHJ7J_000957"
     CDS             complement(56636..57445)
                     /gene="yfcA"
                     /locus_tag="ACHJ7J_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4999228.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFIGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57445..58269)
                     /gene="mepA"
                     /locus_tag="ACHJ7J_000958"
     CDS             complement(57445..58269)
                     /gene="mepA"
                     /locus_tag="ACHJ7J_000958"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO4999229.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58273..59358)
                     /gene="aroC"
                     /locus_tag="ACHJ7J_000959"
     CDS             complement(58273..59358)
                     /gene="aroC"
                     /locus_tag="ACHJ7J_000959"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO4999230.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59393..60325)
                     /gene="prmB"
                     /locus_tag="ACHJ7J_000960"
     CDS             complement(59393..60325)
                     /gene="prmB"
                     /locus_tag="ACHJ7J_000960"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4999231.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            60491..61042
                     /gene="smrB"
                     /locus_tag="ACHJ7J_000961"
     CDS             60491..61042
                     /gene="smrB"
                     /locus_tag="ACHJ7J_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO4999232.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61156..61995)
                     /locus_tag="ACHJ7J_000962"
     CDS             complement(61156..61995)
                     /locus_tag="ACHJ7J_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO4999233.1"
                     /translation="MKIKVILFGMLLFGLNSSVQADVTFNNVQTYGASYGSGLTTSIV
                     YKIDVLTPEGAYYGTYSQGGIMVQAGVVPNLTWTGPGVPPDITIPPGRAPIVDDSYCP
                     GMTSGWVCHRLPVNVHVTGTTHGCPWVVTTSIYSTARPPQAGPYYYQGPNAHQSNCPT
                     EPVASYDISWDENTVVHNKSLSLQSTGGVIEKSLPTFLMKDGRLCDGSDFSDDRGAYC
                     RLVSQLMTFSVQGCDNNKVNVTANRHPVTDKQLHDMQVRVDTSERQSFDATCRFQYIL
                     NEL"
     gene            complement(61996..62514)
                     /locus_tag="ACHJ7J_000963"
     CDS             complement(61996..62514)
                     /locus_tag="ACHJ7J_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4999234.1"
                     /translation="MKTWFALIVMLLLAGNSTARADNNLHFYGNLLSKSCTLVVQGQV
                     LAEVHFPSISSRDLMSREQSDRVPVVFQLKDCKGPVGYKVRVTLTGTEDSDLPGFLAL
                     DASSGAQGMSIGMETVDGIQVPINNSAGAIFNLSNGANNLNFKAWLQAKSGREVTSGE
                     FTASLTAIFEYI"
     gene            complement(62517..62993)
                     /locus_tag="ACHJ7J_000964"
     CDS             complement(62517..62993)
                     /locus_tag="ACHJ7J_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4999235.1"
                     /translation="MKILLYLLLCMSSGIVNASPDITFKGTLVLPPACTISDGNTIEV
                     EFRDVIIDSIDGNNGREVVPYDIKCDAVTPGSSWDMTLTWIGTQTSYDDAAIETDVTG
                     LGIKLRQNGQPFRVNTPIQINADTKPQLEAVPVKAVDAVLTDGTFSASATLRVEYQ"
     gene            complement(62990..63493)
                     /locus_tag="ACHJ7J_000965"
     CDS             complement(62990..63493)
                     /locus_tag="ACHJ7J_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4999236.1"
                     /translation="MRQIFVVGVGILLLGVSGIYNIILANSKPVDLILQVHVDPPPPC
                     SVTGSSVDFQTVNINDINGSNYRKDAGYTLSCPDRKADDLRMQLRGNTTTINGEKVVD
                     VGVPGFGIRIENADNNSLFSIGENNWTPFNITSQPKLNAVPVKQSGAQLSGADFSGSL
                     TMVVDYQ"
     gene            complement(63509..64261)
                     /locus_tag="ACHJ7J_000966"
     CDS             complement(63509..64261)
                     /locus_tag="ACHJ7J_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4999237.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDEQGNKISSPLTVLPPVQRIDAMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKKPWQHKV
                     TLTRSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LSYVNDYGARMPLYFKCGGDTCQVDVEQSRKG"
     gene            complement(64284..66923)
                     /locus_tag="ACHJ7J_000967"
     CDS             complement(64284..66923)
                     /locus_tag="ACHJ7J_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4999238.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAEDEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPSQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDVTHSHTRLDKETAYGK
                     GSLDGNSFRVSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNIMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQSDKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSMAQVDVSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDNAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHQI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(67005..67568)
                     /gene="yfcV"
                     /locus_tag="ACHJ7J_000968"
     CDS             complement(67005..67568)
                     /gene="yfcV"
                     /locus_tag="ACHJ7J_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4999239.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16680 a        18335 c        16858 g        15730 t
ORIGIN      
        1 tcacgcaaag atcattttga tataatttca aaccagtgcc cggttttgat tcctttgtat
       61 aagcatcgtt atatgtctac atcttcatat actggtggta atcccgtgta ttcagtatat
      121 aacagcgata tcatctgtat tggcaacgat ctgacatgtt ggatggaaac ggaattcaac
      181 ctggcattat ctggacatta ccaggcagat aaaaagccag ttaaattttg ggatgatttt
      241 ttataaatcg tgtccgttgt ggcgacgtgg gaaaacctca atattatcct tcacaaattg
      301 cagtaatata gagtctactt cactatccag ttgttcacag tgcttcataa attttttaaa
      361 tttatcatct ttaatgattt tattatgctc tttgccttgt gctcttaact catcataacg
      421 atttctcgcc acaacaataa ctttagccat ttcatctaag cctactacct gtaattgttg
      481 ggggacattg tccatctcat aatataacgc actatccagg ccttcccatc cccaatagtt
      541 acgccattca cataccagca taaaggcttg taattgtgga ggcagcgact ggaatgcctt
      601 aaaaccaaac caaaatttgc atgtcagatt ccattcatat gttattgcta attctatatt
      661 acttaaagca tctccggcat ccttattttt acgtataatc aattccgtca gtgtataacg
      721 ctgttcatca atacgccgaa gagcttctgc catatcgtag tttgccgcca gcaaaacatt
      781 ctctgcctcc tgatgagtga cgtctacttt tgccgtcaat atattgattt ggtcaatatg
      841 aaacaactta atggcttcat caacatcacc accagtcttc tctaacaaac gcctggcagc
      901 ctggataccg acagatatgc tctcccgaag tttgcgaatt tcacttaacc attccatgtc
      961 ataagccttt aaatatttaa gatattatat ataacaatgg tataactgca tttcctgctt
     1021 aacgtccatg ttatgatact cctgaaacta ataagacgta tggcgttgtt tctatttatt
     1081 cgttaatgaa ttcagacaat accattcact tgatgaatga attatttcaa ggaatgatta
     1141 tgacaccttt agaacgcatt acccaacttg ttaatatcaa cggtgatgtt aatgatcctg
     1201 ataccctgcg tccattgtta tctcttgagg atttttttat cggcaataat atcgtcggtt
     1261 cgatatgttg caatgtgact ccagaacaat ctccgcaaac tatctatcac catttacaga
     1321 aaattcgtga gcgtaacgat gtcagtgatg ttttggtaga gattacgatg ttcgatgacc
     1381 cagactggcc ctttagtgaa cgtattttag ttatcacttc tgcttcacca gaagaagttc
     1441 agagctggtt tgtagaagaa attgcccctg atgagtgttg ggaaggatgg tcagaagata
     1501 cgaatcatgg atgggttgag gtccctgcgg gtatgcatcc ggttatttgt tggtgggatt
     1561 aactcttcta atttctttat aaataccata aaaaaaccgc cgaacatgtc ggcggctttc
     1621 tgacttacaa actaacagat tacatcagtg gcattgctaa ccgcacaatg ctaatcagcg
     1681 gttgtggcca tacacccagc accagtacca acagtgcgga gatcagcacc acgataccgc
     1741 ccgcgctgta ctgccagttt gacggtgcat cgcgacccgg ttgttccggt gcgtgcaggt
     1801 acaggctcac cgccacgcgc aggtagtagt agaggccgat tgccgaaccg acaaccacgg
     1861 cacccaccag ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccga
     1921 taaagcccag cgtcatcggg ataccggcca gagacagcat catcaccgtc atcaccgccg
     1981 cgaggatcgg acgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc
     2041 cacgatacgg gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat
     2101 aaccggccag gtaaacccct accgcttcca tcgacatctc gccggtttgc agcgcaatca
     2161 gcgccaccag cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatgt
     2221 tggtctggct cagcgccatc aggttaccga agatgatgga ggcaaaggcg ataatcgcca
     2281 gcaccacccg aatcgcttcg ctgtcaccca ccggtgcgta gaggaacaga cgcatcacca
     2341 caccgaagat agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc
     2401 cctggtatac gtctggcgtc cacaggtgga acggcaccag agagagtttg aagccgaggc
     2461 caacaatcat caggccgaaa cctgccagca acagcggctc gttgagcata ccgtctccga
     2521 ggtttttacc cagcgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac
     2581 cgaacagcag gaaagaagac gctgcggcag aaaggatggt gtatttgata ctggcttcca
     2641 gcgaacgttt ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt
     2701 caataccgag gaacagagac gccagatggt ttgcattcgc cagcaggatc ccgcccagcg
     2761 cggcaattaa caccaacagg tagaactcat ccttgttgtc gttatagcct tcaagccacg
     2821 ggtaggcgaa agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg
     2881 cgaaaccatc aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccaacaaacc
     2941 agagcgaaac cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat
     3001 tgcgtcgcca cgcaatggag agcatcacaa ccaccaccgt caagccgacg atcagcaacg
     3061 gtagcagtgc gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag
     3121 taacggaatt aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa
     3181 tcggctgcgg atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca
     3241 gctcacgcag cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga
     3301 agtaagcgcg atgtaacatc gccagcgaat aaacagatgc aaagaccagc ccaaaggtag
     3361 agataacggt aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga
     3421 cgaagttacc ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacagtgctg
     3481 gcagccattt catcttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac
     3541 gttcataaag ctgaccgcag agaataaaca gacccgccgc tgacaagccg tgcgcaatca
     3601 tctggattac cgcgccctgg taggccaact ggctgccggt gtagatagca atcagtacga
     3661 agcccatgtg ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg
     3721 ccatccacgc accgtagaag atgccgataa cacccagcca catagcgatt ggcgcgaact
     3781 ctgccgacgc atttgggaac agcggcaggg agaaacgcag caaaccgtaa gcggcagttt
     3841 tcagcaagat ccccgcgagg tcaacggaac cggcggtcgg agcctgggag tgcgcatccg
     3901 gcagccagcc atgcagcgga accaccggca ttttgactgc gaaggcgatg aagaagccca
     3961 gcatcaacag atattccaca ccactggaca ttggcgtatt caacagctct tcatagttga
     4021 aggtccagac gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg gcgatcaaca
     4081 tcaccagacc actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt
     4141 taccgtcaga ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca
     4201 tttcccagaa gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc
     4261 ccaggatcca catcaggttg aggtggaaga agccctgata tttttcgatc tctttccacg
     4321 aacagagtac cgccagcaca ccgagcagac cggtcagcac gaccattagc agcgacagcc
     4381 cgtcaatggc gagatggata gagataccaa aacgcgggat ccacggcatg tcgaattcag
     4441 actgccactg cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt
     4501 gcagcgacag cgccagcgtc aatcccatgg tgatcagcgc gatccagcgc ggcaccttga
     4561 cgccaaagcg ttcggtctgc cagcacagga agccgccgat aaagggaatt aatattagcc
     4621 agggtagtaa catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa
     4681 cgaattctca cgacagaaaa ttctcttcgg actgggggta gtgtcggatg caccgcgaag
     4741 gaggcttatc cgacctacgt tcttatatgc ccaggggcaa tcccacaatc cttaactcaa
     4801 cgcagtacca tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac
     4861 cagcgcagat aaccgttctc gcttaacagc aggcctttac ctgcaaagcg ggaaagaacc
     4921 gccggaatgt tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccagg
     4981 aacggcttga cgaacacttt gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag
     5041 gtgcccagca gacggcccgg cgcactgttg gcgatggagg tcaccagagt acgtttaccc
     5101 agccacagcc aggctgccag cagaatgccg accaccgcga ccacgccaga ggtaatttcc
     5161 agggtcaaca tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgcc ctgcagcggc
     5221 ggtacaatca gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg
     5281 tgagttaccc ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa
     5341 atcatacgga aggtgtagag cgaggtcata aacgcaccga ccagacctgc caccatcaga
     5401 ttgatatgac cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca
     5461 gtgaccagcg gcagtgccga cagtgctgcg ccgcccacca ggaagcagag ataaaccagc
     5521 ggaatagatt tacgcagacc gcccatcttg aagatgttct gttcgtgatg gcaggccaga
     5581 atgacggaac cggatgccag gaacagcagc gctttaaaga acgcgtgggt catcaagtga
     5641 aaaatcgccg catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc
     5701 atggtagagt aagcgagaac acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc
     5761 agcagcagcg taaccgcccc gacaataccc accagatgca gaacttccgg cgtcatcagg
     5821 aacaggccgt gggtacgggc gatcaggtag acacccgcgg ttaccatcgt tgcggcgtgg
     5881 atcagcgcgg agacaggcgt cgggcccgcc atcgcgtcgg caagccatgt ctgcaacggc
     5941 aactgcgcag atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcata
     6001 ttattgccgt cagcaaagtg cgctggtgcc agttccacca tttcgcggaa gttcagggtg
     6061 cccagttcgt tgtaaagaat gaacagcgcg aaagcgagga acacgtcacc cacacgggtc
     6121 acgacgaacg ctttcattgc cgctgcgcca ttcttcggat cggtgtaata gaaaccgatc
     6181 agcagatagg agcacaggcc cacgccttcc cagccgaggt acatcagcag caggttgtcg
     6241 gcaagcacca gaactaccat gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag
     6301 tagccctctt caccgcgcat gtaccaggag gcgtacatgt ggatcaggaa acccacacca
     6361 gtgaccaccg agagcatggt cagcgacagg ccgtccagca ccagattgaa accgatgtta
     6421 aagtcgccta ccgacatcca cgtccacagc ggctggctgt aagcctgctc gccgttagcg
     6481 aagaaatcaa cgccgataaa agcggttacc agcgccgcca ggcccacaga gccaacgccg
     6541 acgatcgccg aaacgttttc agaccagcgc ccacgggaga acgccagcag gacgaagcca
     6601 atcaatggca aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat
     6661 cgatgttcag gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg
     6721 cttctgccgc cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc
     6781 agtagcttcc ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa
     6841 tcagcataaa cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga
     6901 ttgccgcgag gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt
     6961 cgcgctgtcg tctttacgat tgctcagcac ttcacccgca cgctcttcac gaccgacgtg
     7021 gaaggccaca accagacccg cgagcagcag catagaagcc agttccaccg ccagtacgta
     7081 aggcccgaac agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccttg
     7141 atcattaaca ccaaggatgg cgtaaacaat caccaccagc atgatggctg acaaaattgc
     7201 cggaccaatc cacacctgcg gtttcagcca ctggcgttct tgttcgattt ctgaaccgcc
     7261 caggttgagc atcatcacca cgaacacgaa cagcaccata atggcacccg cgtagacgat
     7321 aatttccagc gcaccggcga agtaagcgcc tagtgagaag aacaccccgg aaatcgccag
     7381 cagcgaaata atcaggtaca gcagtgcgtg taccggattg gtatgggtga tcactcgcaa
     7441 ggtcgcaagt atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct
     7501 tacggtaaca ggctcttgac gtcgataggc ttggcttcgt tctctgcttc gcccttatct
     7561 ttgccgtcga ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga
     7621 ccggagatca gcagatcctc tttttcgtaa accagatcct ggcgcttgta ttcccccatt
     7681 tcgaaatccg gggttaactg aatcgccgtg gtcggacagg cttcttcgca caaaccacag
     7741 aaaatgcagc gtgagaagtt gatgcggaaa aattccggat accagcgacc gtctttggtt
     7801 tctgcttttt gcagcgagat acagccgacc gggcaggcta ccgcgcagag gttacaggct
     7861 acgcaacgct cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg ataacggggc
     7921 ggcagataga ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg
     7981 ccgatcatcc agatactacg aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc
     8041 atggtcttat tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt
     8101 tgatcagcgt cagcggcagg cagattttcc agccgaagga cattacctgg tcataacgcg
     8161 gacgcggtaa cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcagcg
     8221 cgaaccagat gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca
     8281 tcaatgcaga gatggtcaca atcccgatgt actcacccac gaagaacaga ccgaacttca
     8341 tgccggaata ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa
     8401 acgggtgacg gtgacacacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt
     8461 gcgggatgac gttccacaca tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac
     8521 cggcttgcgc caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct
     8581 gcgcagaagc acgcatcgca cccagcagcg agtatttgtt gttacttgac cagcccgcaa
     8641 acagcaccgc gtaaaccgcc aaaccggcca tcatcaggaa gaacaaaatc ccgatgttca
     8701 ggtcggcaac cacccaaccc ggactgactg gcacaatcgc aaaggccagc agcagtgagg
     8761 taaaggcaat catcggtgcc agggtaaaga tgacgcgatc cgagaatttc gggatccagt
     8821 cttctttaaa gaacattttg atcatatccg cgaccagctg gagcgaaccg ccccagccaa
     8881 cacggttagg tccgtaacgg ttctggaaca gacccagcag gcgacgttcg ccaaagctca
     8941 tgaatgcccc gcaggtgaca accaccagca ggatcaccac cgctttgagg atggtcagca
     9001 ggatctcaat cagttccggt gatatccaac tcattgttgt gcctccttga gatcctcaag
     9061 atgcgcgcca gccagcaccg gagcaatgcc ggacataccc atcggcaagc ccacctgccc
     9121 tgccgtcagc ccttcagcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa
     9181 ggagacgcgt gtacctgcgt tcacacccaa cttcgcggca tccgctgggt tgagtttgat
     9241 gtacggctgc ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcactgcc
     9301 aaacaggtgg taatacggcg caatacgcca tttcccgtcc tgcggctgga agcgtgccgg
     9361 tacgctggtg aagtaatcca gaccattctc gctggtttca aacagacgca cgcccggatc
     9421 gccaaagcgc agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt
     9481 ccagcctggc gcccaggcaa acggcacttg cgaacggtgc gcggtcggct ggttgttacc
     9541 ttccatcgag aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct
     9601 aatattggcg cgcatggcgg tacgaccgct gtaacggtgc ggttcacggg ccagtttctg
     9661 cccacgaata cggaatgtcg catccggcgc agcatctttg atacctgcca gttccgggat
     9721 tttcgccaca acagcgtcaa tcacatggtc gagctgcgtc cagtccactt cacggctcaa
     9781 cagggtactg tgcagcgagt gtaaccagcg ccagctttcc agcatgacag ttttgctgtc
     9841 gtaataagcc ggatcgtaaa ccttgaagaa acgttgggcg cgaccttcat tattaattac
     9901 cgtaccgtcg ctttcagcaa agctggcagc ggaaagtacc agatgggcgt tttccatgat
     9961 cgctgtgcgt tgatggtcaa ccaccatcac cagcggcgct ttagccagcg cagcattcac
    10021 gcgggtagca gaagcgtgac gatgcagatc gttttccagc accaccaccg cgtcggcgcg
    10081 tccggtttcc agttcggtta acgcttcttc aagcgatcca ccgcccataa tgcccagccc
    10141 catgctgttg acggaacggg caatcatggt gataccgacg tcagcgccgc gacctttcag
    10201 ggctttcgcg acgttagccg cagcctgaat tacttcagcg ctaccggcgt tcgtcccgga
    10261 gataatcaac ggtttcttcg cacccgccag tgcctgcacg atgacgtcga ttttgctttg
    10321 cagttcaggt tcaataccgt caaccgctgg cgcggagtta tccagcgcat gggcgatggc
    10381 aaaacctaaa cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc
    10441 cagacgggtg tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt
    10501 gaggattgcc gcaatctgcc agtcagccac tttctgtgct gccgccattt cgcgcgcttt
    10561 acctttcacc gcctgacgca ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc
    10621 gcccagcacc agtaccgcat cgtaagattc gatttcgcgc agagcaggag tataaatgcc
    10681 gccttcacgc agtactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc
    10741 ggtgtagaag ttttcttcgc ccaccagctc acgcagcgca aagttacttt ccacgctggc
    10801 gcgcggagaa ccaatgccga tcactttctt cgactgacgc agaatatctg ctgcgccctg
    10861 cattgcctgt tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg
    10921 cggacgatct ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta
    10981 gtggtttacc gtaccgttgt aacggttttc gatacgacgc agttcgccat aacgctcgcc
    11041 ggggctgatg ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc
    11101 ccatttacgg ttgtaacgct cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat
    11161 ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta
    11221 gacgttgtcg tgcgcgccat aaacgcccag atctgtaccg tcagcgtaat ctttgtagta
    11281 acgcacgcag cggtagcagg cgatgcagcg gttcatttcg tgagagatga acggccccaa
    11341 atcctgatta cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat
    11401 cacggtcata tcctgaagat ggcagttacc gccttcttca cataccggac agtcgtgcgg
    11461 gtggttggtc attaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc
    11521 aatggaaata aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg
    11581 cgtgtcttcc gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc
    11641 cagcgccgga tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag
    11701 caggttgtcc gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag
    11761 catgcttcca gttttctcag tcagagactg agcgttaatc gaaattcggt taccagcgct
    11821 ctttcagcag gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga
    11881 tccccgcctc aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac
    11941 caggtgcgtg ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg
    12001 tttcgatatc gcccggctga ccttcgccac gctccagcgc acgcagaatt ttcacgctcc
    12061 acggcagacc gtcgcggcac ggcgtacacc agccgcagga ctcacgggcg aaaaactctt
    12121 ccaggttacg caccagcgac accatgttga tctcatggtc aaccgccatc gccagcgccg
    12181 tacccagacg gcttcccgct ttaccgatac tttcgaattc catcggcaga tcaaggtgcg
    12241 cttcggtcag gaagtcagtc cccgcgccgc ctggctgcca ggctttaaat ttcagaccat
    12301 cacgcatacc accggcgtaa tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt
    12361 cccacagtcc cggatttttc acccgaccgg agaagcccat cagcttggtg ccagcatctt
    12421 tacttttcga gatgttctga taccactcca cgccgttagc gaggatcgcc ggaacgttac
    12481 acagggtttc gacgttgttg acacaggtcg gtttgcccca tacgccggag gttgccggga
    12541 aggggggttt cgagcgtggg ttagcacgac gcccttccag ggagttgatt aacgctgttt
    12601 cttccccgca gatgtagcgc cctgccccgg tatggacgaa cagttcgaag tcgaaacctg
    12661 ttcccataat gtttttgcca agcaagcccg cttcagtggc ttcggcaatg gcgcggcgca
    12721 gatttactgc cgcttcgata tattcgccgc gcaggaagat gtagccacgg taagctttca
    12781 gcgcaaacgc ggagatgagc ataccttcca ccagcaggtg cggcagttgc tccatcaaca
    12841 ggcggtcttt ataggtgccc ggctccattt catcggcgtt acacagcagg taacggatgt
    12901 tcatggattc gtctttcggc atcaggctcc acttcaggcc agtcgaaaag cccgcgccgc
    12961 cgcgcccttt cagaccagcg tcttttacct gattaacgat ttcgtccgga gacagcccgg
    13021 tcagcgcctt acgcgcccct tcgtaaccgt ttttgctgcg gtattcgtcc agccacactg
    13081 gctgtttgtc atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt
    13141 ttttcattta taccgctcca gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt
    13201 gtcctcatcg atcatcatgt ttggcccttt atcacagttc cccaggcagc aagttggcag
    13261 cagcgtaaag cggccatcaa atgtcgtttg ccctggtttg atgttcagct ttttctcgag
    13321 cgccgcctga ataccctgat acccgttgat atgacagacc acgctgtcgc aataacggat
    13381 cacatggcga ccaaccggct gacggaagat ctgactgtag aacgttgcca caccttcgac
    13441 gtcgcttgcc ggaataccca gcacatcggc gatcgcgtgg atcgcaccat ccggcaccca
    13501 gccacgctgc ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta
    13561 gtggtgcatc tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt
    13621 ttgtggttgt tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata
    13681 ctgcccagat aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc
    13741 tgcaaatgcg cataactcgg cgtgcggata cgagtgcgat aactcatggt gctaccgtcg
    13801 ctggtcaggt agtaactgtt gatccccttg gtcgcctcaa tcatctggaa agattcattg
    13861 gcaggcatca ccggccccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt
    13921 tgcagcgtgc gctctttcgg cggtggcgtg gtcagcgggt gatccgcttt gaacgggcct
    13981 tccggcatgt tgttgaggca ctgctcaaga atgcgcagac tctggcgcag ctcttccact
    14041 ttcagcatta cgcgggtgta gcagtcagaa acaccaccac caaccgggat ttcaaagtcg
    14101 aagttttcat agccagaata aggacgcgcc ttacgcacgt cgaagtcgat cccggtagca
    14161 cgcaggcccg cgccagtggt gccccactcc agcgcctctt tcgcgccata ggcggcaacg
    14221 ccctgggaac gacctttcag aatggtattt tgcagcgccg ctttctcgta agacgccaga
    14281 cgtttcggca tccagtcgag gaactcacgc agcaggcgat cccagccacg cggcaggtca
    14341 tgcgctacac cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt gattgcttcc
    14401 accagatcgt aaattttctg acgatcggta aaggcgaaga acacgggcgt cattgcgccg
    14461 acgtcctgaa taaaggtcga gatatacagc aggtgactgt tgatgcggaa cagttcggag
    14521 agcattacgc gaatgacgtt aacgcgatcc ggcacggtga tcccggccag tttctctacc
    14581 gccagcacgt aaggcatttc gttaacgcag ccgccgaggt attcgatacg gtcggtatac
    14641 ggaatgtagc tgtgccagga ctggcgttcg cccattttct ccgcaccacg gtggtggtag
    14701 ccgatgtctg gtacgcagtc gacaatctct tcgccgtcga gctgcaaaac gatacggaaa
    14761 gcaccgtgcg ccgacgggtg gttcggaccg aggttgagga acatgaagtc ctcgttttcg
    14821 gtgccgcgct tcatccccca ctcttccggt ttgaaggtca gggcttccat ctccagatcc
    14881 tgtttggctt tggtcagctc aaacggcgag aattcggtag cgcgcgccgg gtaatcttta
    14941 cgcagcgggt gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg
    15001 tcgaatgtaa tgccaaacag atcccaggtt tcacgctcat accagttagc gttcgggaac
    15061 agtttggtga aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg
    15121 tcgcggttac gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac
    15181 ccttcgcggt gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa
    15241 ggtttcggca gtttctttaa gaaatcgcca acttccagta attgttcacg cttgatccac
    15301 acaacgggaa ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg
    15361 cgcagttcgc caattaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc
    15421 gcggttaagt cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag
    15481 taagcttcgc gaaatagatt taggaataag cgaagaaaaa tccctttgcc gacaggcgca
    15541 aattaaatct cgtcaggtgt acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt
    15601 tcgcgctctg attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc
    15661 ggacgacgtt ctttgccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca
    15721 ggacgcggcg ggcagcccgg gatatacaca tcaaccggga tgaatttatc gacgccctgc
    15781 acaacggaat aaatatcgta cataccacca gagttggcac aggcacccat tgagataacc
    15841 cattttggtt ccagcatctg gtcatacaga cgctgaataa ccggtgccat tttggtaaag
    15901 caggttcctg caaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg
    15961 ccaaaacgcg ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag
    16021 gaaagaccga agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg
    16081 agcttgccca taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc
    16141 tgcttttgca gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa
    16201 tccatcttaa tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc
    16261 atacgctcgc ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa
    16321 accagacctg ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg
    16381 ctttcgcgga tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg
    16441 aagaacatgg ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag
    16501 tcgataccgg attcaaacgg cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac
    16561 caaccgccta ccagcatcag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat
    16621 gcccagtgat gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa
    16681 gtagcgccag attcactgct cttcacggca gatggacgcc acatcgattc atggggagga
    16741 ataaaaaaaa ccttacaatc actgtagaaa ttcttttata cagctaattg atgtggtctt
    16801 ttactccttt ctataacctt ttgtcaactt taacaaaagt ttcttcacat tagtttacat
    16861 aatatcaaca ccattagcat ttaatgccct ttcaccccag atccttgacg actccaggat
    16921 aattagatgt tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt
    16981 gacaggaatt tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg
    17041 cttttgtcat tccataacaa aaacgcagca acaaatttac gtatttttta acatcattgt
    17101 agcaggtgat ttttttcagg cgattatttg tgcgtacggg gcgtgaatct ctggtgattg
    17161 aaaaatgaac agttttgtac gttctgcacg atgtgaagaa ggttctttac taaaaaaaag
    17221 ccgctgaggt ttaaaacacc cccagcggct cgttttttac actattgtct caggaattat
    17281 ctatggtccg tcgattactc gatatccctt tcaatcaaca atgaatcatc cccttccgga
    17341 gcagatattg ggctgtattg ccacggatta tggtagctct ccatcgcctg atagatcacc
    17401 tgggccagct cattattact ggacgggtca tagcacagca aatattcggt atcaggtagc
    17461 ggcggtaagc catctactcc actcagcaca cgcaagtccg gactcatcat ctcgaccggc
    17521 cttgccgtca cgccaagacc ggctttcact gccgcacgaa cggccggaag cgtcgaggcg
    17581 acataagcca gtcgccaggg aatatctgct ttattaagcg tcgccagcac catatcgcga
    17641 aacggacttg gatcatccag caatacaaga gggatcggct cacctttttg caatacgtat
    17701 tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa
    17761 ggacgatggg tggttaccat caaatccact tcctgggatt ccagcatttc tgccatatag
    17821 gcattacgtt taacgcggac atccagcgct aatttcggat aaaccgaact cacgcgattt
    17881 aacaggaaag gtaagatcgt atcggcagat tcatctgaag caccgatagt taacacgccc
    17941 tgaagattac tgaacattaa tgatgagcag gcctcatcat taaaacgcag gattttcctg
    18001 gcgtaaccaa gaagttgaat gccatgttca gttaacagtt tgttgcgacc gtgacgagcg
    18061 aacagttctt tcccaacgag ttgttccaga cgctgcattt gctgacttac ggcggactga
    18121 gtacgacaca cagcggcagc tgcggcagca aaagtgttca gatcggcaac agcaacaaat
    18181 gttctcagca gatcgaggtc gaggttaatt atcggacgat ttgcacttat catatattat
    18241 cacttactgg cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc
    18301 atttgtaatg tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata
    18361 tgagttgcac taaaaaagcg actcacattg ttccgttata atgcctgaag tagatcacag
    18421 aatatatctt cagggatcgc atatctatta agttactcac tcttttctat ttatgacatg
    18481 cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt
    18541 catacgcgaa cacgtacaaa cagcaatggt gaggcggcat caagagcagg gatccgtcat
    18601 ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg aattatctga
    18661 tgcaaagtta tgttatgtta ggcaaagtaa tcttctatta ttaataagca catcaaaacc
    18721 tttttgaata ttaaataata attaattagc atcatcctca ttcattaatt ccgcttaaca
    18781 atagtttcac accttgcccc cattgcgaca cctcggaaat catcacgctg ttaaactctc
    18841 cgtaatgcgg aaatatcatc atcgcgtttc attagtgaat tcttaaatga ggcattttca
    18901 cacaattatc ttacagataa aaaaaccaga cttacaatta agaatcagaa caattcacta
    18961 tataacatta catgtaaagc atatacacct cattattttg tcattattaa gttattaaca
    19021 gcacaatcga gccttcccct ctggcaaaat cttattctgc agaccttcaa aacaccgtcc
    19081 tgggggagta cattgttcta agctgacttc cacggcaggg agtggcgata acagcaaaaa
    19141 aggtcaagat tcatgtcccc cattgaaaaa tccagcaaat tagagaatgt ctgttatgac
    19201 atccgtggtc cggtgctgaa agaagcaaaa cgcctggaag aagaaggtaa caaggtactg
    19261 aaactgaaca tcggcaaccc agccccgttc ggttttgacg cgccagatga aatcctcgtt
    19321 gacgtgatac gcaacctgcc taccgctcaa gggtattgcg attccaaagg tctttactcc
    19381 gcgcgtaaag ccatcatgca gcactatcag gctcgtggca tacgtgatgt taccgtggaa
    19441 gatatttaca tcggcaatgg tgtatcggag cttatcgttc aggcaatgca ggcattgctg
    19501 aacagcgggg acgaaatgtt ggttcctgca ccagattacc cactatggac cgcggcggtt
    19561 tcgctttcca gcggtaaagc ggtgcattat ctttgcgatg aatcctctga ctggttcccg
    19621 gacctcgatg atattcgcgc taaaattacg cctcgtacgc gtgggatcgt tattatcaac
    19681 ccaaataacc caaccggcgc ggtatattcc aaagagcttt taatggagat tgtggagatt
    19741 gcacgtcagc ataatctcat tatcttcgcc gatgaaattt atgacaaaat tctctacgac
    19801 gacgctgagc atcactcaat tgcgccgctg gcacctgacc tgctgaccat tacctttaac
    19861 ggactgtcga aaacgtaccg cgttgcaggc ttccgtcagg ggtggatggt gttgaacggg
    19921 ccgaaaaaac acgccaaagg ctacatcgaa ggtctggaaa tgctggcttc aatgcgcctg
    19981 tgtgctaacg ttcctgcgca acacgccatt cagaccgcgc tgggtggtta tcagagcatc
    20041 agtgaattta ttacccctgg cggtcgtctt tatgagcagc gtaaccgcgc gtgggaattg
    20101 atcaacgata ttccgggcgt ttcctgcgtg aaacctcgtg gtgcgctgta catgttcccg
    20161 aaaatcgacg ccaaacgctt taacattcac gacgatcaga aaatggtgct ggatttcctg
    20221 ttgcaggaaa aagttctgtt ggtgcaaggg acggcattca actggccgtg gccggatcac
    20281 ttccgcattg tcacgctacc gcgtgtcgat gatatcgagc tgtctttgag caagttcgcg
    20341 cgtttccttt ctggttatca tcagctgtaa tcttaatttc actgccggag attgcatccg
    20401 gcagtgttat cccgccacaa tgacctgatg atgtcatcat acgtaaggtc actatgaaac
    20461 agagccattt ttttgcccat ctctcccgcc tgaaactcat taaccgctgg ccgctcatgc
    20521 gcaacgtgcg gacggaaaat gtgtccgaac acagtttgca ggtagcgatg gtcgcccatg
    20581 cgctggcagc tatcaaaaat cggaaatttg gcggtaatgt caacgccgaa cgtatcgctt
    20641 tactggcgat gtaccacgat gcctcagaag tgctcaccgg cgatctccct actccggtga
    20701 aatacttcaa ttcgcaaatc gctcaggagt acaaggctat tgaaaaaatc gctcagcaaa
    20761 aactggtcga tatggttccg gaagagctgc gggatatctt tgcgccgtta attgacgagc
    20821 atgcatatag cgatgaagaa aaatcgctgg tgaaacaggc agatgcactg tgtgcatatc
    20881 tgaaatgtct ggaagaactc gcggccggaa ataatgaatt cttgctggca aaaacgcgac
    20941 tggaagcgac gcttgaagcg cgtcgcagcc aggagatgga ctacttcatg gaagtatttg
    21001 ttcccagctt ccatctttcg ctcgatgaga ttagccagga ttcaccgctg taagcagcgg
    21061 gagtctgcgt cgcatcaggc aataagcgcc ggatgcgaca tcaggctctt gtcaaaacgg
    21121 aaacagcatc gggatcatca ccacacaaac cgccatcacg ataatggtga acggtacccc
    21181 cagcttcaca aagtcactga agctgtaatt tcccggacct aaaaccagtg tgttaaccgg
    21241 tgaagaaacc ggtgtcataa aggcggcgga tgctgccatc gccacgacca tcgcgaatgg
    21301 ataaggcgac acccccatcg ttttggcagc agccagcgca atcggagcca tcaacaccgc
    21361 cgtcgcggta ttagagataa atagcccaat aaccgccgac aagacaaaca aacaacccag
    21421 catcatatgt ggcccgtaac caccgccaat gtccatcagc cctttcaccg ccagcgcgac
    21481 acctcccgtt ttctgtaatg ccacagcaaa cggcatcatc ccaacgatca aaataatgct
    21541 cggccagtga atggatttat aggcgctttc agcatctata cagcggaatt tccccatcag
    21601 caggcaggcg atgatagcgg caacaggatt aggaatttca tctgtcagca ttaacgccac
    21661 cattagcacc agacagaaaa tggcatgggg tgcctggctg tgcgcgggcg atgcttcact
    21721 cacctcttcc ggtaagttca gcgctacgaa gtcgcggccc tgtttggcca gcataccgat
    21781 cagtttccag ttacccacaa ccaggatgat atcgcccagc agcagaggct catccgccag
    21841 cgaaccttcc agcgccacgc cattgcgctt tagccccacc acattcagtc cgtagcgggt
    21901 acgaaaacca atttcgcgca ccgatttacc aataagttct gactcaggaa ttaatgaaat
    21961 ctctgccatg cccacatcaa gggcctggtc agaaaaatac tcgccgcgca gtaccatcgg
    22021 ctccagcaat tgctcactac aaaattgccg gagatcgaca tcagccgcag acatatcaat
    22081 aagcaaaacg tcacgcgcgc gaaattcaga aaccccatta acgttcacga taacgcgacg
    22141 aaaacgccgc cagcgttcaa caccgatgac gttagcgcca taacgctcac gtaatttgag
    22201 atcgtccagc cgttgaccaa tcattggcga tccggggcga atagccagac gtcgcgcacg
    22261 cccggtcagt cgatattcac ggataagatc gcgaaaggtt cgacgcgtcc agccttcgcg
    22321 ctgcggggtc tgggtatccc ctttcagcat gaaacgcatc actaacatat acaaaatacc
    22381 cagcaccagc acgaccaggc caataggtgt tacgctaaag aaactgaagc cgtgatagcc
    22441 ttcacgcagc aattcactgt tgactaccag gttcggcggc gtcgccacca gcgtcatcat
    22501 gccgcttatc agcccggcaa aacttaacgg catcatcaga cgtgacggcg aagtttgcat
    22561 acgcatggca acgcttaaca ccacgggaat aaagatagcg acaacgccgg ttgaactcat
    22621 aaacgcgcca agccccgcga cggtcagcat caacaaaacc aacattttga tttcactgtt
    22681 gcccgcaact ttgaccagcc atgttcccat tacggtggca acaccggtac ggaccaaacc
    22741 atcgccaata ataaacaagg cggcaatcag gacaacgtta ggatcagaaa agccggaaaa
    22801 tacttctggg actgtcagcg ttccgcttaa tgcaaacgcg acaataacaa acaaagcgac
    22861 cgcatccata cgcactctgc ccgtcgcaaa caagacgatg gcaacggcca gtaatgaaag
    22921 aacccaaatc aattcaccgt tcacaactta tccttgttaa ttgaggggga tgacttgatt
    22981 ctgccataaa aaagccccga cgagacgggg ctaaatcatg atcaggtgtt tcactgaata
    23041 ataacatcgc catttggctg tttggtcaca gtaatttgct ccagactgtg gaggaccaaa
    23101 tcgacctcat tcaggcgcgg ggtatctgcc ggagcgttaa ccgcaatgac atggcaaccc
    23161 gccgccaggc cagaaagcac gccagcggga gcatcttcca ccaccacaca ctcctgcggc
    23221 gcaagcccca gcagctgcgc gcctaacaga tacgcatcag gttctggttt tccgcgcttc
    23281 actcgctcag cggttacaaa cacctctggt gcgggaagcc cagctatttt atggcgcgct
    23341 cgcgctaccg gcatggagcc agaagtcaca atggcccacg gaatacctgc tttattcaaa
    23401 tgattgagta aggcgattgc ccccggaagc gcggtaatac cttcggtttc cgtggcctcg
    23461 atttgctcca gacgcgtaaa ctcggcggca atatcagcct cggatttgcc cgccataaaa
    23521 tggcgcagag aggtgatcgc ctgtttaccg tgaatgaaag ccagcacctc ttccggcgct
    23581 aacccatgac gtctggccca gttgctccac gcccgttcta ccgcaggcag ggaatccacc
    23641 agcgttccat caagatcaaa cagaaaacct ttgcaccgca cgcgggcctc ctcaggcgtt
    23701 gataatttga ttaatttcgt tggcgctcaa atggtactga cgcgggcagg catgccacac
    23761 attaagcatg cgctgatatt tttcccacat tggcgtctgg gcgttaaaac cgtgagttcc
    23821 ggcgtcaaaa tgggtatagc gcccttccac attaaccata aagcggacat aaccgaggta
    23881 acgtgcttca gtggcggcgt caaagccgag gaaggtgaca cgacgttcat cgatggattg
    23941 ctggtcctgc aaattagacc aggaaacatg caacgcatga tacatctcca taatgtcgat
    24001 gatagtgcgg caggtttctt ctttcagctc gccaaactcg cgatccagtt cacgcatctg
    24061 taatccataa ccacgctcaa tgattgtttg caggcgacgg taacgttcag catttgccgg
    24121 atcgagcata gtcatcatct tgtactggtt agacaaaata agacgttgcg cgttggtcat
    24181 ttccattgtt gactcctgta tcactctatt acggtgaaaa aaaagaaggc tgagtatgcc
    24241 ttcttttata tgcgtaatca ggggtcaatt acaaatcatc aaggaaagtt ttatccagtt
    24301 gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta atcaggcttg ccttcaacgg
    24361 gtgccaacac ctgtccagac tcctgcaatt taccgcgaac ttcataaaac cagttgagga
    24421 ttgcaggggg taatggcgtg acagaacgct tgcccagcca ccacaatccc tgcatgggta
    24481 aacttaaggc gaacagggca gtggcaactg ccggcccaag ctgaccgccc agggcaatct
    24541 gccagcagag agtaaatacg gcgatcggcg gcataaaacg gatcgcataa cgcgtcatct
    24601 tgataacgcg attttcgaca aagaccgggg caaggcgttt ttccagcggc cacgtctttg
    24661 agtaatgctg tccccggcga aacaagctaa aaaaattaac agaacgatta tccggcgttg
    24721 acatgcttca cctcaacttc acatataaag attcaaaaat ttgtgcaaat tcacaactca
    24781 gcgggacaac gttcaaaaca ttttgtcttc catacccact atcaggtatc ctttagcagc
    24841 ctgaaggcct aagtagtaca tattcattga gtcgtcaaat tcatatacat tatgccattg
    24901 gctgaaaatt acgcaaaatg gcatagactc aagatatttc ttccatcatg caaaaaaaat
    24961 ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc tctatggctc
    25021 cctgacgttt ttttagccac gtatcaatta taggtacttc catgtcgagt aagttagtac
    25081 tggttctgaa ctgcggtagt tcttcactga aatttgccat catcgatgca gtaaatggtg
    25141 aagagtacct ttctggttta gccgaatgtt tccacctgcc cgaagcacgt atcaaatgga
    25201 aaatggacgg caataaacag gaagcggctt taggtgcagg cgccgctcac agcgaagcgc
    25261 tcaactttat cgttaatact attctggcac aaaaaccaga actgtctgcg cagctgactg
    25321 ctatcggtca ccgtatcgta cacggcggcg aaaagtatac cagctccgta gtgatcgatg
    25381 agtctgttat tcagggtatc aaagatgcag cttcttttgc accgctgcac aacccggctc
    25441 acctgatcgg tatcgaagaa gctctgaaat ctttcccaca gctgaaagac aaaaacgttg
    25501 ctgtatttga caccgcgttc caccagacta tgccggaaga gtcttacctc tacgccctgc
    25561 cgtacaacct gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc accagccact
    25621 tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa ctgaacatca
    25681 tcacctgcca cctgggcaac ggtggttccg tttctgctat ccgcaacggt aaatgcgttg
    25741 acacctctat gggcctgacc ccgctggaag gtctggtcat gggtacccgt tctggtgata
    25801 tcgatccggc gatcatcttc cacctgcacg acaccctggg catgagcgtt gacgcaatca
    25861 acaaactgct gaccaaagag tctggcctgc tgggtctgac cgaagtgacc agcgactgcc
    25921 gctatgttga agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg gacgtttact
    25981 gccaccgcct ggcgaaatac atcggtgcct acactgcgct gatggatggt cgtctggacg
    26041 ctgttgtatt caccggtggt atcggtgaaa atgccgcgat ggttcgtgaa ctgtctctgg
    26101 gtaaactggg cgtgctgggc tttgaagttg atcatgaacg caacctggct gcacgtttcg
    26161 gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc ggtggttatc ccaaccaacg
    26221 aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac accgccagct
    26281 cagctggcgg tgctgttttg taacccgcca aatcggcggt aacgaaagag gataaaccgt
    26341 gtcccgtatt attatgctga tccctaccgg aaccagcgtc ggtctgacca gcgtcagcct
    26401 tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca aacctatcgc
    26461 tcagccgcgt accggtggcg atgcgcccga tcagactacg actatcgtac gtgcgaactc
    26521 ttccactacg acggccgctg aaccgctgaa aatgagctac gttgaaggtc tgctttccag
    26581 caatcagaaa gatgtgctga tggaagagat cgtcgcaaac taccacgcta acaccaaaga
    26641 cgctgaagtc gttctggttg aaggtctggt cccgacacgt aagcaccagt ttgcccagtc
    26701 tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta tgtctcaggg
    26761 cactgacacc ccggaacagc tgaaagagcg tatcgaactg acccgcaaca gcttcggcgg
    26821 tgccaaaaac accaacatca ccggcgttat cgttaacaaa ctgaacgcac cggttgatga
    26881 acagggtcgt actcgcccgg atctgtccga gattttcgac gactcttcca aagctaaagt
    26941 aaacaatgtt gatccggcga agctgcaaga atccagcccg ctgccggttc tcggcgctgt
    27001 gccgtggagc tttgacctga tcgcgactcg tgcgatcgat atggctcgcc acctgaatgc
    27061 gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca ctttctgcgc
    27121 acgcagcatt ccgcacatgc tggagcactt ccgtgccggt tctctgctgg tgacttccgc
    27181 agaccgtcct gacgtgctgg ttgccgcttg cctggctgcc atgaacggcg tagaaatcgg
    27241 tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc atttctaaac tgtgcgaacg
    27301 tgctttcgct accggcctgc cggtatttat ggtgaacacc aacacctggc agacttctct
    27361 gagcctgcag agcttcaacc tggaagttcc agttgacgat cacgagcgta tcgagaaagt
    27421 tcaggaatac gttgctaact acatcaacgc tgactggatc gattctctga ctgccacttc
    27481 tgagcgcagc cgtcgtctgt ctccgccagc gttccgttat cagctgactg aacttgcgcg
    27541 caaagcgggc aaacgtatcg tactgccgga aggtgacgaa ccgcgtaccg ttaaagcagc
    27601 cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc cggcagagat
    27661 caaccgtgtt gcagcgtctc agggtgtaga actgggtgca ggcattgaaa tcgttgatcc
    27721 agaagtggtt cgcgaaagct atgttggtcg tctggtcgag ctgcgtaaga acaaaggcat
    27781 gaccgaaacc gttgcccgcg aacagctgga agacaacgtg gtgctcggta cgctgatgct
    27841 ggaacaggat gaagttgatg gtctggtttc cggtgctgtt cacactaccg caaacaccat
    27901 ccgtccgccg ctgcagctga tcaaaactgc accgggcagc tccctggtat cttccgtgtt
    27961 cttcatgctg ctgccggaac aggtttacgt ttacggtgac tgtgcgatca acccggatcc
    28021 gaccgcagaa cagctggcag aaatcgcgat tcagtccgct gattccgctg cagccttcgg
    28081 tatcgaaccg cgcgttgcta tgctctccta ctccaccggt acttctggtg caggtagcga
    28141 cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg acctgatgat
    28201 cgacggtccg ctgcagtacg acgctgcggt aatggctgac gttgcgaaat ccaaagctcc
    28261 gaactctccg gttgcaggtc gcgctaccgt gttcatcttc ccggatctga acaccggtaa
    28321 caccacctac aaagcggtac agcgttctgc cgacctgatc tccatcgggc cgatgctgca
    28381 gggtatgcgc aagccggtta acgacctgtc ccgtggcgca ctggttgacg atatcgtcta
    28441 caccatcgcg ctgactgcga ttcagtctgc acagcagcag taatctcgtc atcatccgca
    28501 gctttgcgct gcggatatct gaaccggaaa tagtcactat ttccggtttt ttattctctt
    28561 aatttgcatt aatcctttct gattatcttg cttaactgcg ctgcatcaat gaattgcgcc
    28621 atctcacttt gcatacttac cactttgttt tgtgcaaggg aatatttgcg ctatgtccgc
    28681 aatcactgaa tccaaaccaa caagaagatg ggcaatgccc gatacgttgg tgattatctt
    28741 ttttgttgct attttaacca gccttgccac ctgggtagtt ccggtgggga tgtttgacag
    28801 tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca cgcaaagtcg tagatccaca
    28861 ctcatttcgc attctgacta acgaagcagg cgaacctgag tatcaccgcg tacagctgtt
    28921 cacgacgggc gatgaacgcc cgggcctgat gaacttcccg tttgaagggt taacctcagg
    28981 atcgaaatac gggacagccg ttggcatcat catgtttatg ctggtgattg gcggcgcgtt
    29041 tggcattgtg atgcgtacag gaaccattga taacggtatc ctggcgctta ttcgccatac
    29101 ccgcgggaat gaaattctct ttattcctgc gctgtttatt ctgttttcac ttggcggcgc
    29161 ggtatttggt atgggggaag aggccgtcgc ctttgccatt atcatcgcac cgctaatggt
    29221 ccggctgggc tatgacagta ttaccaccgt cctggtgacc tatattgcca cgcaaatcgg
    29281 ttttgccagt tcgtggatga acccgttttg tgtggttgtt gctcagggga ttgccggcgt
    29341 tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg gttatcgcca ctctgattgg
    29401 cctgatcttt accatggtgt acgcctcacg agtgaaaaag aatcctcttc tgtcacgcgt
    29461 gcatgagtcc gaccgcttct ttcgtgaaaa gcaggcggat gttgaacaac gtccgtttac
    29521 ctttggtgac tggctggtat tgattgtcct gaccgccgta atggtctggg tgatttgggg
    29581 cgtgatcgtt aatgcctggt ttattccaga aattgccagc cagttcttca ccatgggtct
    29641 ggtgattggc atcatcggcg ttgttttccg ccttaacggt atgacggtta ataccatggc
    29701 ttcatccttc accgaagggg cgcgaatgat gatcgcccct gccctgctgg tgggtttcgc
    29761 caaagggatt ttgctgctgg tcggtaatgg tgaagcgggt gatgccagcg tgttaaatac
    29821 tatcctcaac agcattgcca atgccattag cggtctggac aacgcggtcg cggcctggtt
    29881 tatgttgctc ttccaggcgg tatttaattt cttcgtgacg tccggttctg gtcaggcggc
    29941 gttaaccatg ccgttactgg caccgcttgg cgatctggtc ggtgttaacc gtcaggttac
    30001 cgtgctggcg ttccagtttg gtgatggctt cagtcacatc atttacccga cctcagcttc
    30061 gttaatggcg acgctcggtg tttgccgggt ggacttccgt aactggctga aggtgggcgc
    30121 gaccctgctt ggactgctgt ttattatgtc cagcgtcgtg gtgatcggcg ctcagttgat
    30181 gggctaccac taaaaatgtt aagagccgca attgcggctc ttttttcatt ctgccgtttc
    30241 agtctctacg gcttcatttt tggcattgcg cttcatccac aacgccagcg ctttcagcga
    30301 gtctggagtg aactcatcgc agcgtgcggt gatttcttcc ggcgtcagcc agcaaacttc
    30361 actgacttca tcttcctgta gggcgaaggg accgtgagag acgcagctga acaacgcgcc
    30421 ccagacacgg caatttttat cttcgaaata gaactgcccg tgctcggcaa aggggacacc
    30481 ggcaatgccc aactcttctt ccgcttcgcg acgtgcggat tccagcagtt gctcatcggc
    30541 ctggactaca ccgcctgcgg tcgcatctaa catgccgggt aaaaagtctt ttgtctcggt
    30601 acgacgctgg accagaattt tgcccatgcc atcatgcacg acgatgtaag ttgcacgatg
    30661 acgcagacac tgtgcccgca tttgctcccg gctggcttgt gcaatgactt cgttctcttc
    30721 attgacaata tccacccatt cagtacttgc caaacgacgc tgttccatta tcaggaaacc
    30781 ttctttttct ggcgctctta cggcgcattt tgagttgtgg gtaaattacg gattaatcgc
    30841 gacctgcgca atgatacttt gatcattgag tgcgattaca ctaagtacgt cattatccag
    30901 catgccataa ctcgccggat taccgccttt cggaatactc accgagccgg ggttgaagtg
    30961 gaaaatctcc ccccgctgtt ctgctactgg cagatgggta tgaccgtaaa ccagcacatc
    31021 gttctggttt aaagcaggca gattttccgg gccaaaaaga tgaccatgcg tcaaaaacag
    31081 acgttgtttt tccagtaata cctgttgcca cggcgcggtt atcgggaaat gcagcagcat
    31141 ttgatccact tcgctgtcgc agttgccgcg cacagcgata accttatgtg ccacttcatt
    31201 aagccgttca gcgactttgg ctggcgcgta accctccggt aaagcattac gcgggccgtg
    31261 attcaacacg tcgccaagga tcaccagcca ctgggcaccg ctttgggcaa acaactccag
    31321 aacacgttcc gtcgccggta acgacccatg aatgtccgat gcaaacatca gtttcatcac
    31381 tcactcctcg tcgaaaaaac gtaagctatg atactggatt ctgtcgttgc tatcagccag
    31441 attgcttcgc tgaaagtgca ataaaacgct ggacagacgc tcgctgccac tggaatgtcg
    31501 cataatccac caggcattct ggcacctcat cgccatgtcg taccaggcgg ttaatcatta
    31561 gcgccagatc agtatcagca atgcaccatt caccaaataa attcggctga cccagtacta
    31621 acaaatgttc tgccatcgcg aacagtttct ctgcactggc ttttccctcg gccgttagtg
    31681 gcgctttttt cgcccccgca aagacaacat ccgtcggacg ctcttcgcgg atgggcatca
    31741 gatcgctgcg caaccaggcc tgaatctgtc gtgcacgcgc acgattttct aaatcaagcg
    31801 gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc gcaatggcag
    31861 aagattcact caactcaaaa tcatcgattt gtaataacgg cacacggcgt gtctgaccgt
    31921 aaccttgcca cgtcggttgc aaatgttcac cgctgtcgag gtcgatggtc ttgatatgaa
    31981 acgacaggcc tttttcctgc aaagccaccc aggcggataa cacataaggg gagaaacagt
    32041 gggcatctga ccaaagcgtg atagcgggtt tactcattat gtcctcggca ataaaggggg
    32101 ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact cacgtaagtc
    32161 gccgcgcttt ctatacttgt ggtgttcaga tgattagaca accggagctg caatgatcga
    32221 tctctatttc gccccgacac ccaatggcca caaaattacg ctgtttctcg aagaagcagg
    32281 gctggattat cgcttgataa aggtagacct ggggaaaggc ggtcagtttc gcccggaatt
    32341 tttgcgcatt tcgcctaaca acaaaattcc ggcaattgtt gatcattctc cagccgatgg
    32401 cggcgaaccg ctaagcctct ttgaatctgg tgccattttg ttgtatctgg ctgagaaaac
    32461 ggggctcttt ttgagtcatg aaacgcgtga acgcgccgcc acattacagt ggttattctg
    32521 gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat cattttaatc acgcagcccc
    32581 ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc tgtaccatgt
    32641 actgaacaag cggctggaaa actcgccctg gctgggaggc gagaactaca gcattgcgga
    32701 tattgcctgc tggccgtggg ttaatgcctg gactcgccag cgaattgacc tcgcaatgta
    32761 tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg ggcaggcact
    32821 gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg ttctcgtgta
    32881 ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc cgctattatt
    32941 cgtataaaga accttcgttt acgtacattt atcggaatta aggaagaaga aattaacaac
    33001 cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc gcgcactagc
    33061 gaagatatca acgatgcgct gaattatcgc accgtaacga aaaacattat tcagcatgta
    33121 gagaataacc gtttctcttt gctggaaaaa ttaactcagg atgtgctcga tatcgcacgt
    33181 gaacatcact gggtgacgta tgctgaagtg gagatcgata aactgcacgc gctgcgctac
    33241 gccgattcgg tatccatgac cttaagctgg cagcgttaac cgccatatcg ggaggctgca
    33301 tgaacatagt gatcaccgga gggacgggat taattggtcg ccatttgatt ccacgtttgc
    33361 aggagctggg ccatcaaatc acggtaatga cgcgtaaccc gcagaaagcc agttccgttc
    33421 tcggccctcg ggtgacacta tggcaagggc ttgccgatca aagcaacctc aacggcgttg
    33481 atgcggtaat caacctggcc ggagaaccga ttgctgataa acgctggact cacgagcaaa
    33541 aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat ttgattaatg
    33601 ccagcgacac gccaccgtcg gtactcattt ccggctcggc aacgggctat tatggcgact
    33661 taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttacc cataaactct
    33721 gcgcccgctg ggaagaaatt gcctgccggg cgcaaagtga caaaacgcga gtgtgcctgc
    33781 tgcgtaccgg tgtagtgctg gcaccggatg gcggtattct cggtaaaatg ctgccgccgt
    33841 ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca gtatctggcc tggattcata
    33901 tcgatgatat ggtcaacggc attctctggc tgctggataa cgagctgcgc gggccattta
    33961 atatggtttc gccctacccg atacgcaatg aacaatttgc ccatgcgctc ggtcatgcgc
    34021 tgcatcgccc ggctattttg cgcgtccccg caaccgccat tcggctgtta atgggcgaat
    34081 cttcagtact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa gaagcgggtt
    34141 ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga tgtcgttcgc tgatgtggtt
    34201 tactgcaaac atccgccagt taactcccgg tgttacaggg ttagtggctt tgcgcgataa
    34261 gatcgtctgg tgaaagtcgg gtcagcgtca ggatgacttc gtggtcaaat cctttttcca
    34321 gcatctcctg agcaatacgc agagcttctt cgtgtttgcc ctgcattgcg ccttcttcac
    34381 gtaatctgtc agcaatggtc atcagtttct ccttttcttg tggtgcgcgt tctgttatct
    34441 caccaataaa tgcacggaaa cgctgggcat ctcccgtttg taatacataa ttaaacaggg
    34501 cttttagctg tctgtcatta gtgttccctg taactagcag cgaaacaatt tggtcgacta
    34561 atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc gccattttgc
    34621 gatgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga aaagccgatg
    34681 aatatatttt acgggcaatc gcaggctcgg caaattcatc aagccagcag agtgaataag
    34741 gataaggact tctgcaacca tgataaaaca gcatcgggat caccaatgga agctctttat
    34801 agcccgcatc aagatggttt tgcattgccg caatggaata acgcattatg cgaaaagcca
    34861 ttaattcttc cggcttactt tggtgctcta tcactacata aatataaccc actccctcct
    34921 gcgttttcac agaccacaag aggtcggaat aatattgccg caggtcttca tcaataaaac
    34981 tgtttggttc cagtttaagc gtcgttaaat cacacagttt gcgcagcggc gcgggaagat
    35041 gaatatcaat aaaatcccgc gcggtgtctg gatggcgtaa aaaagattta aataccgcat
    35101 catgcggcgt ggaagttgtc gatattgtca tggcgttccg tcacccctca aatttaagat
    35161 gacgcgacaa taaccacgat taacacccaa tgcagcaggc agtttttatc tttggacagc
    35221 gaattcagaa gtatttttag cggactgcaa cttttacatt tcagcggaag ctgactcgca
    35281 aattcccctt gaaaatactc cggcagcgtt agccaccgga gaggcaatta tttcagcgat
    35341 cccttaagga actgttgcag acgagtgctt tgcgggttgc caaataattg ttctggcgct
    35401 ccctcttctt ctattttccc ctgatggagg aaaatgacgt gagtagaaac atggcgagca
    35461 aagcccattt cgtgggtcac caccaccatc gtttttccct cttccgccag ctgctgcata
    35521 atgcgcagca cttcgcccac cagctctggg tcaagggccg atgtcggttc atcaaacaac
    35581 agaacttccg gttccatcgc cagcgcccgc gcgatagaaa cacgctgttg ctgaccgccg
    35641 gaaagatgca ccggatattt cccctgtgca cgttcgtcta tcccgacttt tgccagatac
    35701 ttcaccgccc gctcgcgcgc ttcctgcttg ctcaggccca acacctgaat cggcgcttcc
    35761 atgacgtttt ccagcaccgt catatggctc cagagattga agtgctggaa taccatcgtc
    35821 aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga ctttgagttg accgtctttg
    35881 tcgcgcacca gattgatcgt ctggccattg accacgatcg acccttcact cggtttttcg
    35941 aggaagttaa tgcagcgcag aaaggtactt ttccccgatc ccgacgatcc gatgatgctt
    36001 atgacatctc cggcattcgc ttgcagtgac acccctttca gcacttcgtg ttcgccgtag
    36061 cgtttgtgca aatcgataac gtttaattta ttttcggaca tcgtattctc agtgcgttga
    36121 agaaggtttc acatgctgca accagcgttt ttccgctctg cgaaagaggc tgatcaggac
    36181 ataagagatg attaaataga gcaccgcggc aatgccgaag gcggtaaaag gttgatacgt
    36241 ggcggcgtta atatcgcggg ctattttcag cagatccggc accgtggcag taaatgccaa
    36301 cgcagtagag tgcagcatca ggatcacttc gttgctgtat gccggtaacg caatacgcag
    36361 cgcagaaggc aaaataatgc agcgatacat tttaaaagtc gagaagccat aggctctggc
    36421 ggcttcaatt tccccatgcg gaaccgaacg gattgcccca gcaaaaatct cggtagtgta
    36481 agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc aggccactgc ggaaaaaggc
    36541 gttaagaaac tcggtcccct taacaatttc cagcgtatac atgccggaat agaacaccag
    36601 caactgaaca tacagcggcg taccgcgaaa aatataggta aataaccaga ttggaaactg
    36661 gatgtattta ttactggaga cacgaccaat cgccagaaac agcgccagaa ctccgcctat
    36721 cactaccgac aaaataagca gccacagagt gatcgccacg ccggtaaagc gataaccgtc
    36781 ggtccacagc agcggtttcc agtattcatg taagatttcg atcacaggtc agccctcttc
    36841 acacccacgg agtagcggcg ctcaaggaac agcagcacac cattggaaac ggtggtgaaa
    36901 accaggtaaa tcacgccaca gacgatagcg aaatagaacg gttcccaggt acttttgcct
    36961 gccagctgcg tggctttgac cacatcttcc aggccgagta acgaaaccag tgcggtagat
    37021 ttgaggatca cctgccagtt gttgccaatg cctggcaggg cgtaacgcat catcgccgga
    37081 aacatgatcc gccgaaacac ttgcccacga gtaaaaccaa acgccgtcgc cgcctctata
    37141 tgtcctttcg gcactgccat aaaagcacca cggaacgttt cggtaaaata agcaccgtag
    37201 ataaaaccga gagtgataat accagcgacc atcggatcga tatcaatctg cccgacgccc
    37261 atcgcctccg tcaccgtgtt tagcgcaatc tgcaaaccgt agaaaatcag cagcatcaac
    37321 actaagtccg gcacgccacg aatcagcgtg gtgtaccctt cgaaaatcag ccccgaaagc
    37381 cgattttgcg agagcttacc gccagcgcca attaaaccga tgattacagc gagcactaca
    37441 gagctgatag ccagctctag cgtgacgagc gcaccctgta aaataacacc tgaaaaccca
    37501 tacaacatgc tgcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc tccctccggt
    37561 tacccgaagg gagcgggctt tctttcaccg atggcgatta accaccataa acatcaaaat
    37621 cgaagtactt tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg gcaaaggctt
    37681 tgttcagtgc ctcgcgcagt tcgttatctt ctttacgcag gcccatgccg gtccctacgc
    37741 caaacagttt ttcatcttta acagacgggc caccgaattt gtaatcttta ccgacaggtt
    37801 gtttgaggaa accttcgctg gcagcgacct catcctggaa cgcggcatca atacgtccgg
    37861 cagtcaggtc agaataaatg ttgtcctgcc cctgatacga gacgatttca atgccttttg
    37921 gtgcccaatg ttcattaccg aacgtctcct gggtggtgcc ctgcaatacg cctacccgtt
    37981 tgcctttcag cgactcgact gtcggctgaa tgtcagaatt tttcgccacc accaaacgag
    38041 aatcggcagc gtacagtttg tcggtgaagg ctatttcttg ctgacgtttt tccgtaatgg
    38101 aaagcgatga catgatggcg tcaatcttct tcgcttttaa ggacgggatt aacgcatcca
    38161 gcggattttc gacaaacgta cattgcgtat tgatgcgttt gcataattcc tttgccagat
    38221 cgatatcgaa gccaaccagt tcgccttgtg aattttttga ttcaaatggc gcataggtcg
    38281 ggtcggcacc gatgcggatg ttttgcggaa tcgcagcaaa tgccgcagtt gcgctggaga
    38341 aggccagaac cagagagagc gatagcacca gttttttcat aatgtcctca actgacagtc
    38401 tttttatagg gaatatttac aggtttgtta cacttatcgt gccataaaat tgaccatcaa
    38461 ggcaaaatat cccgccccgt taggcgtatt atttttcatt aaaaatgctt aagtatgcat
    38521 ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc atgatgatca ttccatcagg
    38581 cacagcttcc ctgagacgta tcagtcaccg tagacattaa agtcaaaata ctttttcgcc
    38641 atcttgtcgt aagtgccgtc ctgacgcagc tctccaagcg ccttattaaa cgcagccgtc
    38701 agttcagtat catctttacg tagccctaca ccggtgccat caccgaagta ttttttgtct
    38761 ttcactgatg agccagcaaa ggcgaagtct ttaccagcag gttgcttgag gaatccttcg
    38821 ctggcagcaa cttcatcctg caatgcagca tccagacgtc ctgcagccag atcggaatag
    38881 accaaatcct ggttggcata ggccaccaca tccacacctt tgctacgcca ggtctcgtta
    38941 gcgtaagctt cctgggttga tccctgcagc acaccaacat gtttaccttt cagtgaatcc
    39001 agcgttggct gaatcggcga acctttggcg gcaatcaaac gagaatctgc ggcgtacaac
    39061 ttgtcggaga aagcaatctc ctgctgacgt ttatcggtaa tggagagcga cgaaataatg
    39121 gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt caaagtcact ggcaacccag
    39181 gtacatttca cctgcatccg tttgcacatc tcgttaccga gatcgatatc aaagccaaca
    39241 aaatcacctt tagcatcttt cgacgagaac ggcgcatagg tggtatcggt tccgatacgt
    39301 accgtctccg gtagcgccgc atagctggaa gccgctgcgg agagaccgac taacaaagac
    39361 agagcgagaa tcgacttctt catacataac cctcaagtga aaatggcttt tttatgttgt
    39421 gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct tatgccatct tgacgacaac
    39481 agcaacattc aacgttaaat gtgttaataa gacgttgcat tattgtcctg aagttgaaga
    39541 tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat ggcagaaaca aaatgtcgag
    39601 gatttgatcg cggttgcaaa attgccctga aacagggcaa cagcggggtt atgcgccctg
    39661 ccagcgggca aagagatctt caggaagggt tatcgcaaac tggtcaagaa cacgattaac
    39721 cgtctgattt atcacatcat caagggattg agggcgatga taaaacgccg gaacgggagg
    39781 cataatcacc gcaccgattt ctgctgcctg cgtcattaaa cgcagatggc ctaagtgcaa
    39841 tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct ttcagcacaa catctgccgc
    39901 acgggtcagt aagccatcag tatagctatg gacaatgccg gacagggttt tgattgaaca
    39961 gggtaaaatc accatcccca gtgtctggaa agaaccggaa gagatgctgg cggcaatatc
    40021 gcgcgcatta tgcgtgacat cagctaatgc ctgcacttcg cgcagggaaa aatccgtttc
    40081 gagggataag gtctggcgcg ccgcctggct catcaccaga tgcgtttcga tatctgtgac
    40141 atcgcgcaga acctgtaata gacgcacgcc ataaatcgcg ccgctggcac cgctgatgcc
    40201 tacaatgagt cgtttcataa atagttgccc tgtcagactt gcaggcagac tttgcaggat
    40261 ttcggcggga gttgcaagtc agggtgccag atcggcaccc tgagcgaagg catcatcctt
    40321 cgttatgcat ttcgagattt tccacttcgt tctgacgttg cactgctttg gcgtcgtcat
    40381 tacgtaaagt atcgaggaaa tcgaggtagc cctgatcaac atctttggtg acgtagacgc
    40441 cgttgaacac cgagcattca aactgctgga tatccgggtt ttcagcgcga acggcttcga
    40501 tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg cgaatttcat
    40561 ctacttcgcg accgtgagcg atcagttccg tagcgctcgg catatcgata ccgtaaacgt
    40621 tcgggaagcg aatttccggt gccgcagaag cgaggtacac tttcttcgct ccggcttcgc
    40681 gtgccatctc gataatctgc tcagaagtgg tgccacggac gatggagtcg tcgaccagca
    40741 ggacgttttt atcgcggaac tcggcgcggt tggcgttcag tttacggcgc acggacttac
    40801 gacgcagctg ctggcccggc atgatgaagg tgcggccaac atagcggttt ttaacgaagc
    40861 cctggcggta cggtttgccc agaatacgag caatttctag cgcgatatca cacgaggttt
    40921 ccgggatcgg gatcaccacg tcgatatcca gatcttccca ttcgcgggca attttctcgc
    40981 ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga aattttgtcg atgaacgagt
    41041 ccgggcgagc aaagtataca tactcaaaca ggcacggatt gctgaccgga ttgtcagcac
    41101 attgacgggt aaacaactgc ccttcttcag tgatgtaaat cgcttcgcca ggcgcgacat
    41161 cacgcaggaa atcaaagccc agtgtatcga gcgcaacgct ttcggaagcg accatatatt
    41221 ctgtgcggtt ctcgtcaata tcacgttttc ccagtacgag cggacgaata ccgttcggat
    41281 cgcggaaagc aaccataccg tggccgataa tcatcgccac acaggcatac gcgccgcgga
    41341 ttaagcggtt tgtagcagcg atggcggcga aaatattgtc ggcttccagc gggtagtggc
    41401 ggaagttgtc cagctcgctg gcgaagatat taagcagaat ttccgagtcg gaagtggtgt
    41461 tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg ttggtcagat
    41521 tgccgttgtg ggcaagcgta atgccatacg gggagttaac gtaaaacggc tgcgcttcag
    41581 aggcgctgga gctgccagcc gtggggtaac gcacatgacc aatgcccata ttgccctgca
    41641 aacgctgcat atggcgagct tcaaatacat cgctcaccag cccgttcgct ttacgcaaac
    41701 ggaagcagtt attggcatct atggtgatga tgccggcggc atcctgaccg cgatgctgaa
    41761 gcaccgttaa ggcatcataa atcgactggt taaccggcat aacaccggcg ataccgacaa
    41821 taccgcacat acgtcttttt cctcgttaag ccacatctca gagcacttac gctctgggca
    41881 agaaacttga cgagctttgc agataatcaa aaaaccatct gatgataaaa ctgaactgcg
    41941 ggatcagctg ggatttgctc cagtcttcgc ttttcgacac cccggtaaag gagtcgagaa
    42001 agaagagaat ggcagcaaca atcaacacac cgcgcaacgc accgaaacag acgcccagca
    42061 cccgatcggt gcctgacaac cccgttttct ccaccagctg gcctatcacg aagttcacga
    42121 tagcaccaac gatcagggta gcgataaaca gtaccgcgat ggcaatccca tttcgaacca
    42181 gttcgtcttc aaagcccgta aaccagactg acaggtaagt gtagtaatga ctggcaacaa
    42241 agaaagcaca accccatgtc accagcgata acgcttcacg aacaaagccg cggatcaggc
    42301 taaccagaga ggaaaaagca atcaccacga ttatggcgta atcaatccag accatatgtg
    42361 tcccacgatt ttacgccctg tcatcctgtt cggggcgcat tctaacagaa aaagaaaacg
    42421 tttgcgtagg gatttccttc ccgcgcatca ataaaaatgg cgctgaaaaa atattcaacg
    42481 ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt gtcggatgcg gcgtaaacgc
    42541 cttatccgac ctacggttct acccctgcgt aggcctgata agacgcacca gcgtcgcatc
    42601 aggcaacggc tgtcggatgc ggcgtgaacg ccttatccga cctacggttc tacccctgcg
    42661 taggcctgat aagacgcacc agcgtcgcat caggcaacgg ctgtcggatg cggcgtgaat
    42721 gccttatccg acctacggtt ctacccctgc gtaggcctga taagacgcgc caggcgtcgc
    42781 atcaggcaac ggctgtcgga tgcggcgtaa acgccttatc cgacctacgg ttctacccct
    42841 gcgtaggcct gataagacgc gccagcgtcg catcaggcaa gaccgtatta attcggcgta
    42901 tagcccatta ccacgccact taagccagaa agttgcttca actcacccag cgatcctttc
    42961 agcttatctt tcgaggcatc tggcccaacc agaatacggg taattttacc ctgcactggc
    43021 gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac aatctcattc
    43081 actttatcgg catttttcag cgcccccagt tgcacaacat aggctttacc cgtcggtgca
    43141 gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtgc ttcaaccttt
    43201 ggtttaggcg gctccaccgg tttcggcttc ggtggggcga ccggtgcagg ttccggttca
    43261 aactcggtgt tattggctgc aatagtggcc ggatcgagcg acggtgctgc ggcatcacct
    43321 gcccgcacct cttccgctgc gccttccggc ggctgcgtcg gcaacgcctg ggtggcggct
    43381 ggcatcatat caggctcatc gcgatcgccc gcttttggca ccagcgggat agccgcaaac
    43441 tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat cacccccagc
    43501 gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac cgcttctcct
    43561 cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa gaaccacaca ccagcacggt
    43621 gtcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgcaacgc tatcaaatga
    43681 tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg gcccttccag
    43741 tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc cggcaatatc
    43801 tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta gcgctttcat
    43861 acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa taacgcgtgg
    43921 cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc cgtcgcgaat
    43981 ggcattttca ctgacctcca gcccgctggc acgcagtgcc gccagcgccg ttgcggcatt
    44041 cggttgcggg acaagcggca acggcaggtt ttccagcgtg ccgtgagcat cgctaaacgc
    44101 ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta acagtgcacc
    44161 tttttcctgc gccacatcag caatggtaga aggcatttcc ggctcaccga caattgccgg
    44221 tttttcgctg cggaagatgc ctgctttctc gcgaccaata ctttcgcgat ctggccccag
    44281 ccagtcggta tgatccagcg caatactggt tactaccgcg acatcggcgt cgacaatatt
    44341 ggttgcgtcc agacgaccgc ccagccctac ttccagaatc accacgtcaa gttgtgcctg
    44401 tttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg aaatatcacc
    44461 gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc gattccggca attccttacc
    44521 ctgcacacgt acgcgctcgg tataacgcac cagatgcggc gaactgtaga cgcccacttt
    44581 gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt tgccattcgt
    44641 acccgcaacg gtaaatacaa atggcgctgg tttcaggacg ccaagacgcg ccgcgacctg
    44701 gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca gataagaaag
    44761 ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg tatccgctga
    44821 ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta tcagttatca
    44881 ggcctcaggt tcctgatccg gtaccggggg taccactacg ccttcacgcg gcgcttcagg
    44941 attcggcgct ggcagattca tcaacttcgc cagaatgctc gccagtttca ggcgcatttc
    45001 cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc aggaattcac tgcgctggaa
    45061 tcctggcggc agtttttcgc gaacggtctg ttcgataaca cgcggaccgg caaagccgat
    45121 taacgctttc ggttcagcga tgttgagatc gcccagcatg gcgaaacttg cagaaacacc
    45181 gcccatcgtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct gcatttttgc
    45241 cagtgccgca gaagttttcg ccatctgcat cagcgacatc agtgcttcct gcatacgtgc
    45301 gccaccagag gcggagaagc agatcagcgg gcagttatct tccagcgcct gctcaacggc
    45361 acgcacgaaa cgtgcaccca caacagaccc cattgaaccg cccataaagg cgaactcgaa
    45421 tgccgcagcg acaaccggca ttccatacag agtgcctttc atcaccacca gcgcatcttt
    45481 ttcgccggtt tctttctgcg cagatgccag acggtcttta tacttcttgg agtcacgaaa
    45541 cttcagcaca tctttcggct caagctcgct acccagctcc acaaggcttc cttcatctaa
    45601 caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact tcggacagac
    45661 ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat cacacttagt
    45721 ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt taattcgttc
    45781 aatccagctc attagggacc tttctgtctg aacctggttc gatgccagtt ttatctttgg
    45841 ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt aaaacataac
    45901 agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta ttttgcggcg
    45961 cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc ccggcaaaat agaaaccaca
    46021 ataatcccga cgatcagcag cttaaggtta tcctgaacca tcgggattgt accgaagaaa
    46081 tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc cgatcacgtt ataagcggcg
    46141 aaatgacggt acgacatgtg gcccattccc gcaacaaacg gggcgaacgt tctgacgatc
    46201 ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt tctcataaaa ctgatgggtt
    46261 ttgtcgagat aactgcgacg gaaaattttc gaattgggat tactgaataa cttttcaccg
    46321 aacaaccgcc cgatagtgta attgaccgcg tcacccacaa tcgcggcgat cagcatcagc
    46381 accaccatca tatggacgtt gagatcgttg gtttccagtg atgccaacgc tccggcaaca
    46441 aacagcaatg aatcacccgg taaaaacggc gttaccacca gaccggtttc acagaacaaa
    46501 attaagaaca aaatggcata aacccagacg ccgtactccg cgaccagctc tgccagatgc
    46561 acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt tgcctatgcc
    46621 ttgtactcgt catctttcag gctgtaacta cgttggctac agcctgaaat gttccgagca
    46681 ttattctcgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa gatcataccg
    46741 gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg ctgccgccag
    46801 cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt tgtgggcacc
    46861 gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg cattcgcttt
    46921 gatatctacc accacataag gaccgtgacg cgtgacatta atatgcataa cgttgcgcca
    46981 cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct cgcccagcaa
    47041 gcattgcgca gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat gggttacccc
    47101 tttactcagt accgccgggc gcagccgatg attgtagatg atatagcgat aacggcgagc
    47161 cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac gcacggcgat
    47221 gtcaccaggt aaattcgcat ttacgcccaa cgtccacgca gcgtctttgc gctgcgcggt
    47281 tgtttcgaaa tgcaccacct gcccggtacc gtgtacccct gcgtcagtac gcccggcgca
    47341 gaagacggtg atgggttcgt tcgccacctg ggagagcgcc ttttccagct tctcctgcac
    47401 actgcgaact tcgttctgcc gttgccagcc gtaatactta ctgccgtcgt actcaatgcc
    47461 cagcgcaatt ttataaactg gcggttgttg ctggtcggac attagtacag atactcctgc
    47521 accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac gttatcggcc
    47581 accgaccaga actggacttg ctccggcata ccgtagtcat tacgcacgca gccaacagaa
    47641 agatgcggcg tacccgaagc atcccctacc tgagttggga attcgttctc ttcagagagc
    47701 acaatatctt cgccttgaac aaacgcatca cgcgcttctt ctgctgccag tggacgcaga
    47761 gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac gacgcttgcc
    47821 gaaatcatca gtccttcgtc ctgcaggatt ttgcgtactt cgtcaacgat acgacgttct
    47881 tcacgcacgc taccttcgct atccggcagt aacggcagca tgttgaacgc cagctgacgc
    47941 ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca atttagcact ctgccccgct
    48001 aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt aacgctgata
    48061 cgtgataaac cgccctgatc gattaacggt ttcagtgccg ccagcagctg gctggtcaga
    48121 ctgtccggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa cgggtttact
    48181 tccggcacca ccagcggtac gtcgggttcg agagcaaaca atccgctgct gtcgatcacc
    48241 aggcaacctg agttggtcgc ttcttcaacc caggctgcgg tagcttcttt gcctgcgaca
    48301 aaaaatgcca gctgcgcctg cgtccagtcg aattcagcag catcctgcac ggtgattgtc
    48361 ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc ataaatttcc
    48421 ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccac agcgccagtt
    48481 gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag aaatagcaaa
    48541 gctccctgcc aatgttacag cagagagcat gaagaagaga ttaacgtgcc ggatgatgaa
    48601 cggcgttaaa acccagttta cacagcaatg atgccgcact ggcgtcatca caaattacat
    48661 acagagatga ccattcacgg cgctcaagat agtttttgcg cagtttatcg aactcacccg
    48721 gtatcccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac accaaatgca
    48781 ccagcctttt cagcgtcggt tgatcgagcg ggccatgcag cgtaatgcga ccaaactctg
    48841 gcgcgggcag taatgtatcc agcgcaacgt gctgttcatg cccaataaac ttgctatagg
    48901 cttcaaacac ttgcgtagta ccgcgtgctt taccttccag ggtatagcct gcaatatgcg
    48961 gcgtgccgat atccactttt gtcagcaatt ccacattaag ttccggctcg ccttcccaga
    49021 catccagcac cacgcttaac ttctggcctt cattcaggca ggtcagcagc gcggtattat
    49081 cgacgactgc gccacggcag gcgttaatca gaatcgctcc cggcttcagg ctacggatca
    49141 gtttttcatc cgccagatgc agcgttttgt acggcccgtc tttgaagagt ggcgtatgga
    49201 aagtcagaat atccgcatgc tgaactaatt catccagcga gcggaaatca ccctcatccc
    49261 cacggtcagc gcgaggcgga tcgcaaagta aggttttaat ccctaacgct tccagtcgtg
    49321 cctgtaaacg ccgaccaacg ttaccaacgc ccacgatccc cacggtacgc tcgtgcagtg
    49381 aaaatccatc gcgttcggca agcatcagca gggaggagaa aacatattcc accaccgcaa
    49441 tcgcattaca gccaggtgca gcggaaaaac caattcccgc ctgcttcaac catgcttcat
    49501 cgacatggtc ggtccccgca gtggcagtgc caacaaattt aatgggtttt cctgccagca
    49561 aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct gccagttgag
    49621 cgacggggat tggacgcccg ggaaccgcga tcacctcacc caaacggcta aataagtcgc
    49681 gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct gtatgagacg
    49741 agagttaacc ggacaagtgt gccataatct cgtggccagg catacttgcg aagatttcag
    49801 gtataaggat acgtaatgat acaacctatt tccggccctc ctcctgggca accaccaggt
    49861 cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca gcaacgtact
    49921 tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacagcaaag agcagaactg
    49981 tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct ttcgcgtcac
    50041 ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg ccgcgcaaaa aagccattct
    50101 gcccgccagc ttttagcgca attaggggag tatttacgtc tggggaataa tcgtcaggcg
    50161 gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct ctcaccggag
    50221 caattaaaaa ccattctcac cctgttgcag gaagggaaga tggttattcc gcaaccgcag
    50281 cagcgcgagg cgaccgatcg tcctctatta ccggcggagc acaatgcgct caaacagctg
    50341 gtgactaaac ttgcggcggc aacgggggag cccagcaaac agatctggca atcgatgctg
    50401 gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa ccatctggtg
    50461 acctggctgc aggcgcgtca gacgctaagc cagcaaaata cgccgacgct ggaatcacta
    50521 caaatgacgc taaaacaacc tttagatgcc agtgaattgg cggcgttatc ggcatatatc
    50581 cagcaaaaat atggcctttc tgcgcaatca acgctttctt ctgcccaggc cgaggatatt
    50641 cttaatcagc tttatcaacg gcgggttaaa ggaattgatc cgcgtgatat gcaaccgctg
    50701 cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg tcccgcgctg
    50761 tggatactgt tagtcgcgat tatcctgatg ctggtctggc tggttcgtta accccgacga
    50821 aatgacagta tcgtgacaat aattcccagc accgcagaga tcgccccggc aagaaatacc
    50881 gaagagtaac caaacgtggt cgccagcatt cccgccagcg gcccggagac gccgagggcg
    50941 atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga ggggacacgt
    51001 ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc ggttaacgcc
    51061 gcgcctgcta atgcgaccca cgcacctggg gcttgccaga gcagcaacaa gcccaccgtt
    51121 tctacaagca gagagactat cgccactttc acgccgccaa aacgatccgg catccagcca
    51181 aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg taagagtaaa gcccgccatc
    51241 gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc aaaaccaacg
    51301 ccttgtagtg ccagccctaa ccctggtttc cagataagcc cgacaacgct ccacagagat
    51361 ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc ccacgccagt
    51421 aagggtaatg ccattgtggt gagcgccagt gcggcaaaac cgtaatggct atgaatcaac
    51481 aggccaagcg gtgcaccaac agcgagggca ccgtaaatcg ccattccatt ccatgacatc
    51541 actttgccag agtgttttgg ccctacgatg cctaatcccc aggtcagagc gcctgtcagt
    51601 aactggcttt caccaaagcc aagaatcaaa cgcccgatga ccaacagggc aaatttgaac
    51661 ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc cccccgccag accacaagct
    51721 aacatcccct gaagcgccga acgttttgcg ccatattgat cggccagtcg cccggcgtaa
    51781 ccacgcgtca gcaccgtagc cagaaactga atgccgacgg caatgccgac catggtattg
    51841 ccatagccca attcatgatg aacaaacaac gggataaccg gcaacggcaa ccctacggtc
    51901 atgtaggtga gaaaaaccgc aaaagcgatg cggaagagcg aaaaattggc agaagatcgt
    51961 gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa ggcgaggagt
    52021 atccccgtct catctctctg gtttcagggt tacagtgcgt tggcaggaat taacgcgtac
    52081 gtcttttcag aaggaaatcg acaaagcggg aagtttgcct ggaacaggcg gcgattgtca
    52141 atgatgtgaa aaagggaacc atcgggttcc ctttggcgtt agtgccggag gccgcattgg
    52201 cgcgtaacgt cggatgcgac gctgacgcgt cttatccgac ctactccgac tactccgacc
    52261 tactgcgaat taatctttca gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc
    52321 gaagctgtta gacataacag tggtcagttc gcgatcggtc gtttcggtca cgatgttcag
    52381 acccgcagcc tgctcgtcca gctcttcaat gttgatgctc ggggcgataa agccgtgttc
    52441 cagcatcagc agagagtaga tagcttcctg tacgccagca gcgcccagag agtgaccggt
    52501 catggctttg gttgcagaaa tcgccgggct cttatcgccg aacacttcac ggatagctgc
    52561 cagctccttc acgtcgccaa ccggagtcga agtaccgtgg gagttcaggt aatcgattgg
    52621 ggtatcaacg ccatgcatcg ccatctgcat acaacgtact gcgccttcgc cagacggagc
    52681 aaccatgtct gcgccatcag acgttgcgcc gtagccaacg atttcagcat agatgtgagc
    52741 accacgcgcc agcgcgtgtt ccagctcttc aaccaccacc ataccgccgc caccagcgat
    52801 aacgaaaccg tcacggtgag cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta
    52861 tttagtagac agcgcaccca ttgcgtcgaa ttcgcaagcc atttcccagc acagctcttc
    52921 gccgccgcca gcaaacacga tgtcctgttt gcccagttgg atctgctcta ctgcgttacc
    52981 gatacagtgt gcggaagtcg cacacgcgga gctgatggag tagttaacgc catgaatttt
    53041 aaacggggtg gcgaggcagg cagaaacgcc ggatgccatc gctttggtga ccacatacgg
    53101 gccaaccgct ttcaggccgc gcgggccgcg cattgcgtca gcgccgaaca cctggaaacg
    53161 cggggagccg ccgccggaac ctgctatcag gccaacgcgc gggttattct ggtaagcttc
    53221 cggagagagg cccgcatcag cgattgcctg ctccatagaa aggaatgcat aaatggatgc
    53281 gtcgctcata aagcgcacaa ctttgcggtc aatgaggcca gtggtatcca gttttacgtt
    53341 gccccagacg tggctacgca tgccggaatc cttcagctcc tgagagaaag tgatccctga
    53401 acgtccttca cgcagagatg ccaggacttc ctgctggtta ttaccgatgc tggaaacaat
    53461 gcccaggcca gtaatcactg cacgtttcat tcaatacctc tgtaagtcgc acatagagta
    53521 agtttcgaat gcacaatagc gtacacttgt acgccgaaca agtccgatca gccaattaat
    53581 agagaaattt gcgcagcctt acacacatcg ctaagatcga gccaccgcct gtaagacgag
    53641 taacttacgt gaaacactac tccatacaac ctgccaacct cgaatttaat gctgagggta
    53701 cacctgtttc ccgagatttt gacgatgtct atttttccaa cgataacggg ctggaagaga
    53761 cgcgttatgt ttttctcgga ggtaaccaat tagaggcacg ctttcctgag catccacatc
    53821 ctctgtttgt ggtagcagag agcggcttcg gcaccggatt aaacttcctg acgctatggc
    53881 aggcatttga tcagtttcgc gaagcgcatc cgcaagcgca attacaacgc ttacatttca
    53941 ttagttttga gaaatttccc ctcacccgtt cggatttagc cttagcgcat caacactggc
    54001 cggaactggc tccgtgggca gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt
    54061 gccatcgttt attactcgat gaaggccgcg tgacgctgga tttatggttt ggcgatatta
    54121 atgaactgac cagccaactg gacgattcgc taaatcaaaa agtagatgcc tggtttctgg
    54181 acggctttgc gccagcgaaa aacccggata tgtggacgca aaatctgttt aacgccatgg
    54241 caaggttggc gcgtccgggc ggcacgctgg cgacatttac gtctgccggt tttgtccgcc
    54301 gcggtttgca ggacgccgga ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg
    54361 aaatgctttg tggggtgatg gaacagacat taccactccc ctgctccacg ccgtggttta
    54421 atcgtacggg cagcagcaaa cgggaagtag cgattatcgg cggtggtatt gccagcgcgt
    54481 tgttgtcgct ggcgttatta cggcgcggct ggcaagtaac gctttattgc gcggatgaag
    54541 cccccgcgct gggtgcttcc ggcaatcgcc agggggcgtt gtatccgtta ttaagcaaac
    54601 acgatgaggc gctaaaccgc tttttctcta atgcgtttac ttttgctcgt cggttttacg
    54661 accaattacc cgttaaattt gatcatgact ggtgcggcgt cacgcagtta ggctgggatg
    54721 agaaaagcca gcataaaatc gcacagatgc tgtcaatgga tttacccgaa gaactggctg
    54781 tagccgttga ggcaaatgcg gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca
    54841 ttacttatcc gcaaggtggt tggctgtgcc cagcagaact gacccgtaat gtgctggaac
    54901 tggcgcaaca gcagggtttg cagatttatt atcaatatca gttacaggat ttatcccata
    54961 aggatgactg ttggttgctg aattttgcag gtgataagca agcgacgcat agcgtggtgg
    55021 tactggcgaa cgggcatcaa atcagccgtt tcagccaaac atcatctctg ccaatatatt
    55081 cggttgccgg tcaggtcagc catattccga cgacacccga actggcaaaa ctgaagcagg
    55141 tgctgtgcta tgacggttat ctcacgccac aaaatccggc gaatcagcat cattgtattg
    55201 gtgccagtta tcatcgcggc agcgaagaga cggcgtacag tgaagacgat cagcagctga
    55261 atcgccagcg attgattgat tgtttcccac acgcacaatg ggcgaagacg gttgatgtca
    55321 gtaaaaaaga ggcgcgttgc ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag
    55381 gtaatgttcc cgattatgag gcaacactcg tggaatatgc gtcgctggcg gagcagaaag
    55441 ataaggcggt aagcgcgccg gtttttgacg atctctttat gtttgctgct ttgggttcgc
    55501 gtggtttgtg ttctgcccca ctgtgcgcag agattctggc ggcacagatg agtgaagaac
    55561 cgatcccgat ggatgccagt acgctggcgg cgttaaatcc gaatcggtta tgggtgcgga
    55621 aattgttgaa gggtaaagca gttaaggcgg ggtaatctgc tttggcagtg tttgtcggat
    55681 gcggcgtgaa cgccttatcc gacctacgtg tgacgtgtag gcctgataag acgcgataag
    55741 cgtcgcatca ggcatggtgc tccagacgcc gcaacttact gctgtgatgc ctgctgaaac
    55801 aggttttccc acatatcggt caccagcgcc tggtcacgcg gcgacaattc accagcacca
    55861 atggcttttt ccagactctg gctaacggtc gtatgcaccg cctgagcgga gtggtcatca
    55921 ccactttcca gttctgcgat ggctaatgtc aggtggccac gcaaataccc actggcaaac
    55981 aactcatcat cactggcatg gtcaaccata ccgtcgatta atgccagaat gcgtgattca
    56041 aactccgcga tcatcttctt tcctcattgt aaaacgtggc gctggcttca gttgagctct
    56101 tccggccacg ggaactgttc cgccgtaagt tccggcgtgt gataataatt ttgtagtgct
    56161 ttaatgaaac gtgccgggcg ttccggaatg ccatttgtta gataatccat cacctgcgca
    56221 tgtactcgcc gttgaaacac cacgcggtcc ggttcgaagt cgccttccag attgtcgcaa
    56281 ctgacgttaa acggatatcc agccgccacg cagaacaacc agtcgagcgc ctgcggcttc
    56341 acttcaacat cttcaaactg gctttgcgtc tgggcatcgc gtccgtccgg gcaataccag
    56401 tagccgaaat caaccagctc acggcgcgct tttccggcaa tacaccagtg cgaaatctca
    56461 tgaattgcgc tggcataaaa gccatgagca aagacgattc ggttatacgg tacttccgca
    56521 tcagcaggaa gatagatcgg ttcgtcgtcg cctttaatca gacgggtatt aaaatcatcg
    56581 gcaaagcagc tattaaaaat ttcaatcaac tgctcgtagt ggtgtgtact gttcattagt
    56641 tcatccccaa ccagtggagg atctcctgtc catggctatc gtaaagtaat ttggcactca
    56701 tcaccgccga gacgataacg atcatcgggc ggatcagttt ttgtcctttg ctcaacacca
    56761 gtcttgaacc catgcgcgcg ccgaggaact gccccaccag catcacaaag cccgtcgccc
    56821 aaatcacttt gccgccgaga ataaacagca gtaaaccgcc gatgtttgac gttgcgttga
    56881 gcaatttggc atgagccgtg gctttggcga ggttaaatcc gcacagcgta acaaaggcca
    56941 gcgcgtaaaa cgatccggct gccggaccaa agaatccgtc ataaaaaccg acgcagccac
    57001 cggcaattaa cgcgaagggt aaaccgtaca tccggcgctg gcgatcttct tcacccagct
    57061 ttggcatcag caagaaatag aggccgatac aaatcaccag aatgggcaaa atctgccgta
    57121 agacatcagc ctgaacgtat tgcaccagca gtgcgccgct cattgagccg ataaaggtca
    57181 tggcgatatt gagtttctga tcgcttaaac taaccacttt gcggcgaata aagtagatag
    57241 tggcggaaat agagccgccg caggcttgca gtttattggt tgccagcgca ttagcgggag
    57301 acatccccgc tgccattaat gccggaatcg tgagtaaccc accaccaccg gcaatcgagt
    57361 cgataaatcc cgccagcatg gcgacaaaaa agagaactcc cagcaacagc ggggaaacca
    57421 taaacaggct attaaacgtt tccattagat cacgtgctca tccagtagcg cctggcagga
    57481 aggcggcaac ggaggcggtg tcttcttctc aggctttgtt gttcccggtt ttggaggttc
    57541 aaaccagctt tgcagttctg ccccgcaacc atcgcctggt ggcggtaaag gttgatcttc
    57601 acactccaga ctatcggcag gacaacgtaa tcgtacatgc atatgcgcac gatgctggaa
    57661 ccagggtcgc actttgcgca accagtcgcg atcggtgccc gcatcaaggc aaagttgttg
    57721 tttaatcgcc ggattaacaa aaatgcgcgt gacgtctttg tcctgggcgg cgagtttgat
    57781 caagctgaaa atttccggct tccacagcgt ggagacaacg tgtttaccgt cgcgggaaac
    57841 taagtccagt gcttgcgggc gcaagagctg cgcggaggtc cagcgagttt tcggcagttg
    57901 cagaaagata tcgacatcca gtccggtctg gtggctggcg tgaccgccgt tgaaacgccc
    57961 accagcaggc attcccatat cgccaatcaa caccgtaccc atgcccagat tgctcacctg
    58021 gctactcagg cgctggataa acatcaccag gtccgggtga ccgaaatagc gacgctgatc
    58081 ggtacgcatg acctgataat gttcggactg tatcggcaac gtgtcagcgc cgacgataca
    58141 accattggaa aaactgccta tcgattgtgc gctacccggc acaggttggg ttattttttg
    58201 ccacggcgtc gctgccaggc tgacgctact ggcaagcagg gccagcagcg caatcgcggt
    58261 tttgttcatt ttttaccagc gtggaatatc agtcttcaca tcggcatttt gcgcccgctg
    58321 ccgtagcagg tgatccatta aaacgatcgc cagcatcgct tcagcgatcg gcaccgcgcg
    58381 gatcccgaca caaggatcgt gacggccttt ggtgatcatc tcaacttctt cgccaaagcg
    58441 gttaatggtg cgccccggca cggtaatgct ggaggtcggt ttcagcgcca tatgggcaat
    58501 gatttgctgc ccgctgctga taccgccaag aatgccgccc gcatggttgc tctggaaacc
    58561 gtctttggtg atttcgtcgc gattctggct gccgcgcagc gccactacgt caaaaccgtc
    58621 gccaatttcc acgcctttca ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc
    58681 caggcggtca aagaccggct caccaagccc ggcaggaacg ccactggcaa caacggtgac
    58741 tttcgcgcca atagagtcgc cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc
    58801 gattttgtcc gggtccgggc agaagaacgg attttgttca acctgcgacc agtctttgat
    58861 ttccagcgga atgtcgccca tctgggtcag gcagccgcgg atttcaatac caaatttctc
    58921 ggcgagatat tttttggcaa tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga
    58981 agagcgtcca ccgccgcgat aatcgcgcag accgtatttt tgttcgtagg tgtaatcggc
    59041 atggccagga cggaaaacgt ccttaatcgc actataatcc tgagaacgct gatcggtatt
    59101 ttcgatcaac aaaccaatgc tggtgccggt agtaacacct tcaaaaacac cggagagaat
    59161 tttgacctga tctggctcac gacgctgggt ggtatagcgc gatgtcccag ggcgacgacg
    59221 gtcgaggtca tgttgcaggt ccgcttccgt cagcggaatg cctggcggaa caccatcgac
    59281 gatgcagccg agcgccagcc cgtgcgattc gccgaaggtg gttacgcgaa agagttgtcc
    59341 aattgtgttt ccagccatca cggctccgtt attgttgtgt ttgcgtgttt acttaatctt
    59401 tataaatcgc gaaatgttca cgggccgcca gcagctgcgc tttggtgagc ataaacacac
    59461 catcgccgcc gttatcaaac tccagccagg tgaacggaac atcgggatat tgttccataa
    59521 gatgtaccat gctgttgccg acttcacaaa tcagtacgcc atcatcagca agataatctg
    59581 ccgcgtttcc gagaatgcga cgcgtcagtt tcaggccgtc ggtgccagat gccaggccca
    59641 gttccggctc gtgacggtat tcgtttggca ggtcggacat atcttccgca tcgacatacg
    59701 gcgggttagt gacgatcagg tcgtactgca ctttcggcaa atcacggaac agatcggaac
    59761 gaatcggaat gacgttgtgg atcagaccgt gttcttcgat gttctgttcg gcaaccgcca
    59821 gcgcgtcggg agagatatcc accgcgtcga cttccgcatc cgggaaggca taagcacagg
    59881 caatggcgat gcagccgcta ccggtacaca tatctaaaat atgctgcggt tgcttgctga
    59941 taagtccggc aaatttattg ttgatcaatt caccaatcgg cgagcgcggc accagcacgc
    60001 gttcatcgac gtaaaattca tggccgcaga accacgcttt gttggtcagg taagctaccg
    60061 gaatgcgttc gttgactcgg cggatcacgc gctcaacaat acgatgtttt tcgctggagg
    60121 tcagacgcgc ggtgcgcata tcttccggaa tatccagcgg caggtagagc gacggcaaca
    60181 ccagttgtac cgcttcatcc cacgggttat cggtgccgtg accgtaccag atatttgccg
    60241 cgctgaagcg gctaaccgac cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg
    60301 cttcatcaac gaaaatttta tccacgtatt cctccagggc atgatcgcaa taatttcggc
    60361 ggctagtttg ccatgaagat gacgataaat cagcattcac gcgcggtgag tgaggaaaaa
    60421 tacgtttaaa acgatcgatt gcgctacgag tcgggtaaac tgtaggaaaa ttagaaatag
    60481 agacagataa atgaaaaaga aaacaacact tagcgaggag gaccaggctc tgtttcgcca
    60541 gttgatggcg gggactcgca agattaagca ggacacgatt gtccaccgac cgcagcgtaa
    60601 gaaaatcagc gaagtgccgg tgaaacgctt gatccaggag caggctgatg ccagccatta
    60661 tttctccgat gagtttcagc cgttattaaa taccgaaggc ccggtgaaat atgttcgccc
    60721 ggatgtgagt cattttgagg cgaagaagtt gcgacgcggt gattattcgc cggaattgtt
    60781 tttggattta cacggtctga cgcagctgca ggccaagcag gaactgggcg cgttgattgc
    60841 cgcctgccgt cgtgaacatg tgttttgcgc atgcgtaatg cacgggcacg ggaagcatat
    60901 tttgaaacag caaacgccgc tgtggctagc gcaacatccg catgtaatgg catttcacca
    60961 ggcgccgaaa gagtacggcg gagatgcagc gttgttagtg ttgattgaag tggaagagtg
    61021 gctaccgccg gagttgccgt gaatattagg gggcggatgc gttttgccat ttcgccctga
    61081 atgctgggac caaatgccgg atgcggggta aacgccttat ccggctacga acactgtcga
    61141 ggctaataat attaattaaa gttcattaag aatatattga aagcgacatg tagcatcaaa
    61201 actttgccgt tcactagtat caacacgcac ctgcatgtca tggagttgtt tatcagttac
    61261 aggatgtcgg tttgccgtta cgttcacttt attgttatca caaccctgga cactaaacgt
    61321 cattaattgt gataccaggc gacaataggc tccacggtca tcactgaagt cactgccatc
    61381 acataatcgc ccatctttca ttaagaatgt cggcaacgat ttctcaataa ccccccccgt
    61441 agattgcagg cttaatgatt tattatgaac aacggtgttt tcatcccagg aaatatcata
    61501 gcttgctact ggctctgttg gacaatttga ttggtgcgca ttcggcccct gataataata
    61561 tggccccgcc tgcggaggcc ttgctgtgct ataaattgat gttgtcacca cccaagggca
    61621 accatgggtt gtacccgtaa catgtacatt tacaggaagt ctatgacaaa cccaaccaga
    61681 ggtcataccg ggacaataag aatcatctac tatcggtgcc cgccctggcg gaattgttat
    61741 gtctgggggg acacccggtc cagtccaggt taagttaggc acaactcctg cctgtaccat
    61801 aatcccccct tgtgaatatg tcccataata tgccccttca ggggttaaca catctatttt
    61861 ataaacgata gatgttgtta gcccactgcc ataagatgcg ccataagtct gaacattatt
    61921 aaatgtaacg tctgcttgta cagaagaatt aagcccgaac aataacatac caaataaaat
    61981 cacttttatt ttcatttaga tatactcaaa aattgccgtc aggctggcag taaattcacc
    62041 agaagtgact tcacgcccag atttcgcctg caaccaagcc ttaaagttaa gattattagc
    62101 accgttactc aggttaaata ttgcccctgc cgaattgttg atggggacct gtatcccatc
    62161 aactgtttcc atgccaatgc tcataccttg cgcacccgat gatgcatcca aagccagaaa
    62221 ccctggcaaa tcgctatctt cggtaccggt taacgtaact ctaactttgt aaccaacagg
    62281 ccctttgcaa tctttcaatt gaaaaacgac cggcacacga tcagattgtt cccgtgacat
    62341 taaatctctg ctgctgatac ttgggaaatg gacttcagcc agcacttgtc cttgtactac
    62401 cagggtacaa gatttactta ataaatttcc gtagaagtgc aaattattgt ctgcacgcgc
    62461 tgtgctattt cctgccagta acagcattac tatgagtgcg aaccaggttt tcatcttcat
    62521 tgatattcca cccgtaatgt ggccgaagct gaaaacgttc cgtctgttaa tacagcatct
    62581 accgccttca cggggacagc ctctaattgt ggtttcgtat cggcgttaat ttgtatcggt
    62641 gtatttactc taaatggctg accattttgc cgaagtttaa tccctaatcc tgttacatca
    62701 gtttcgattg cagcatcatc ataacttgtt tgtgtgccta tccatgtcaa tgtcatatcc
    62761 caggaactac cgggtgtaac agcatcacat ttaatatcgt agggcacgac ctctcgccca
    62821 ttatttccat caatgctatc gataattaca tccctgaact ccacctcgat agtgtttcca
    62881 tcactaatag tacatgccgg aggcaataca agagttcctt tgaaggtgat atcaggtgat
    62941 gcattcacaa taccgctgct catacagagc aataaataca agagtatttt cattgataat
    63001 ccaccaccat tgttaaactc ccactaaaat cggcccctga aagttgtgca ccactttgtt
    63061 ttaccgggac tgcattaagt ttcggctgtg atgtgatatt gaaaggggtc cagttgtttt
    63121 ctccaatgga gaatagtgag ttgttatctg cattctcgat tcgaatacca aatcctggaa
    63181 cccctacatc aacgactttt tctccattaa ttgtggtggt gtttcctctc aactgcatac
    63241 gtaaatcatc agcttttcgg tcagggcatg acaacgtata tcctgcatct ttacgataat
    63301 tactaccatt gatatcgttg atattcacag tctgaaaatc tactgaactc cccgttacgc
    63361 tacatggcgg aggaggatct acatgtacct gtaaaatgag atcaacaggt tttgaatttg
    63421 caaggataat gttatatatc ccactaacgc ccagcagtaa tatacccacc cccacaacaa
    63481 aaatttgtct catgtcgctc tcccggcatc agcctttgcg gctctgctct acatccacct
    63541 ggcaggtatc accgccacat ttaaaataca gcggcatccg tgcgccatag tcgttgacgt
    63601 aagacagcac cgggacgcta tccattttga cctttaacgg cacggaggtt tttggtgata
    63661 tcaccatggg ggaaaatccc gctgccggat tgccattttt ctgcgcactg gcgttactga
    63721 taaccacgaa gtacggcgtc gggttattga cggtaaactc ctgaccgctg cgggttaagg
    63781 tcaccttatg ctgccacggt tttttcatgc tgacgttttc cagcgccttt ggtcgccaga
    63841 acagcttgat gcgcgtttgc agcgctatct gtaacgtgtt gggtttgttc gatttcggcg
    63901 gaatttcacg cacgttgaag tagaacacgc tctcgcggtc ggcaggcagt ttgttgatat
    63961 ctggcatgcc ctgtaccttc acctgaccgt tcatcatggc atcgatgcgc tgcaccggcg
    64021 gcagtaccgt cagtggcgag ctaatcttgt taccctgttc atcttccatc cagctttgtg
    64081 ccagatacgg cattttcggg tcgttattgc gcagcgtgac gctgattgat ttatcacttt
    64141 cgttaaaaat caggcgtgta cggtccggcg tgacagaggc ctgtactgac aggctggcgg
    64201 cagttatcag taacgccagc gtcgttgcgg ccgttctggc agagcaaaat agattcgtca
    64261 tggatcattc cttaatcacg ttgttaattg gcggataccg ataccggcgc ttccgttggc
    64321 atcacctgct gggtctgctc ctggatcacc ggcttaattt gatgaggcat cgaaggagat
    64381 atcgcccctg tttgttgaca tggtagtaac aggccattga acagatcggc tggcagcggg
    64441 tcaggcagat gaatatcgca gtgggccaca ccgccccatg aaacgatcat atgctcgcct
    64501 ggtttgacac ccgcgaggta gacgttgccg tcatcatcaa ccagaccgac gttctgcgcg
    64561 ttgtcatttt tcacttccgc gccaaacggc ggatagctgc catcttgcag acgcaagacc
    64621 gccatcgctt tttgcccgct gatcaccgag aacttacggt agccaatggc cccttcggta
    64681 agcgtcgcct gcaccacgga ctgggtggct tcggcgtttt ccggcaggtt gttaaggtcg
    64741 atatacgcct ggttgcggta gtagttgttg acgtctgcca ccacggcctt accgaacata
    64801 ttggtgtaaa ccgccgcgcc atttccttcc accggaacac cggccacgcc atcggcatct
    64861 attaacagac gcgtgccgcc catattctgg gtacggtgga gcgcgccccc ctgcgcggtg
    64921 agcgtcgccc cgccctggag tgaaatcccc gccgaggtgt actgtccttc atggtagttg
    64981 gcgctgacgt ccacctgcgc catcgaacca tcgtggctgt aatagccatc aacgctggag
    65041 tgattgtcgc tggtgcctac gttcaactgg taatgggttg cgtcatcgac acggctgaaa
    65101 taccccacct gactgctgtc cgaaccgctg ccgtagttgc cgttatagct gatggtgctg
    65161 ctgtcacccc acggcatgct gagcgatatg taaacacctt tatcggactg gttgtcatac
    65221 tcgtagcggt atccggtcat ggaaatgctc atgttgcgga tactgcccag gttgaagtag
    65281 tgcgagagca tgatgttgta gttagtctgt tcgtcgcgat cccagtaggt atggcgggtg
    65341 tagttgagat aaacagacac accggcatcc ctgaagttct ggttataggt ggcggtgtac
    65401 atctctttgt cgttgccggt gcgcaccatt tcgctgtcgc tggcatcgag atactcgctc
    65461 atggtcatga agttctcttc cgagaagcgg tatccggcaa aagtgacgcg gctgttcagt
    65521 tcatcgaagt ctttggaata gctcacgcga aacgagttac cgtccagtga acctttaccg
    65581 taggcagttt ctttatccag gcgggtatgc gagtgggtga catcaaaggc caccgcacca
    65641 aacacagaca gatcgcgacc gacgccaagc gccgccgact gatagtgttc atctgccagc
    65701 gccccgccgt acagcgacca gccgttggca atcccccagg aagcttcgcc accggagaag
    65761 aaaccgcctt caacatgatg cccccactcc tgcgggcggc ccatcatcag tttatagcgc
    65821 acctggccgg ggcgggtcag gaacggcatc gaggcggtgt tgatgtcata ttcctgcacc
    65881 tgaccgttct gttcttcaat gcggatatgc agcgtaccgg agaccgaatc gccaagatcc
    65941 tggatgcgga acggccccgc cgggacctgg gtttcgtaga tgacgcggcc caactggctg
    66001 acggtcactt tcgcggtggt gtgcgccacg ccggagatat ccggcgcata gccgcgcaga
    66061 ttgggcggca acatttgatc gtcggtgctg atactgccac ccacgtagtt aaagccgtcg
    66121 aaaatatcag aattcaggta gtcttcgcca aggccaagtt tggcttttag cgacggtaag
    66181 gctcgccagg cgtagtagcg gctccactcc cagttttttt gcgtgtcatc accgttgata
    66241 acatcgtcat cattgctttt actatgcaaa taatcagtct gccagtcggc acgaagacgc
    66301 catgcgccga ggttcacccc aaccgtcccg ttaccgctaa tttcattgct gtcatccccg
    66361 ccattttctt catgtcgtgt ctgggcggta atactgtagt cagcaattaa accagatata
    66421 ccgtcatccc agcgtgaagg cggatcccag ttgatgtcgg tatattcaag gtaagcctgg
    66481 ggtaatgaaa tgactaacgc tgactgactc aggtcagctt taatatcaat gccttccagt
    66541 tgactgggtt tcaggcattt gccgtcgtgg atccattgca ggttttttgc cacatcctct
    66601 tttaagccaa actgcgcgac cagttcaggc gtcaggcagg cataggtttt actggcatcg
    66661 ttctcagagg cgtaccagta aatatcgtat tcttccgtca gcggctgttt atttagttga
    66721 acctgtaaat tgtatttccc aggctcgaca taaccctggc tggaaaatcg cttcagatcg
    66781 atttttgtgt cgccttttaa ctccagaaaa cgggaatcaa actgaatttc atcttcagcc
    66841 caggcattga cataactccc ggacattgcc aatgcaacgc agcagggaag tacccgcagt
    66901 cggaaaagag aatggtcagg catacacgta tccatccgta attattataa tttgcgcgaa
    66961 ttccatttaa aaaccccata gcccgaaagc tatgggggta tcacttagag ataagtaatc
    67021 tggaaggtcg tgttggcttc aaaattacct aaatctggcg catcagcagc gcccaccagc
    67081 caggctttaa agtccagcgt ctgcttcgct ttgcctttgt tggtcgccgt atcgtttacg
    67141 attttctgtt cgatacccgc gccgctttta taagaggttc cctgagcgtc accaatggcc
    67201 aggctgacat tgttaaatgc cgcaccagta tcggtattgt aaatggtgta gtaattgccg
    67261 ctgttggtag aagacgcgtt accggtgaag gtagtggtca tcgttgtctg ggtgtcaaac
    67321 acacagtctt gcagatggat ctggaaagct ttcggtgtag aagtcccgtt gtttttcagg
    67381 atccctgaac caatgtcacc catatcaact tccagtttgt gatcatcagg aacgatggag
    67441 cacggggaat cttcaatggt gccgtagaaa cgtgctgtac cattgttgcc agcggcgatt
    67501 gttgaagtag aagcgaacgc acccattacc attgttgcag caatagctgt ttttacaaac
    67561 ttactcatta tttgtcctta aaccataaat aattaaaaat ata
//
LOCUS       DAZFFG010000011        66890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFFG010000011 DAZFFG010000000
VERSION     DAZFFG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.011"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..724)
                     /locus_tag="ACHJ7J_000969"
     CDS             complement(<1..724)
                     /locus_tag="ACHJ7J_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO4999240.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDSLSPKQK
                     QTTLCCFWNKIASSFPEPYNSTITHNIIFYIDKENLMMRGTISIVDEVVKTYSLPIEK
                     DDNGHYDFSGLYLAHSNISGKDPNKDADKDFGIDMGNCNCSNVNFEHTYFDGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCT"
     gene            complement(955..1929)
                     /gene="yajO"
                     /locus_tag="ACHJ7J_000970"
     CDS             complement(955..1929)
                     /gene="yajO"
                     /locus_tag="ACHJ7J_000970"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO4999241.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLDMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELEATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(1984..3846)
                     /gene="dxs"
                     /locus_tag="ACHJ7J_000971"
     CDS             complement(1984..3846)
                     /gene="dxs"
                     /locus_tag="ACHJ7J_000971"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO4999242.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRLMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(3871..4770)
                     /gene="ispA"
                     /locus_tag="ACHJ7J_000972"
     CDS             complement(3871..4770)
                     /gene="ispA"
                     /locus_tag="ACHJ7J_000972"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO4999243.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(4770..5012)
                     /gene="xseB"
                     /locus_tag="ACHJ7J_000973"
     CDS             complement(4770..5012)
                     /gene="xseB"
                     /locus_tag="ACHJ7J_000973"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO4999244.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5218..6666
                     /gene="thiI"
                     /locus_tag="ACHJ7J_000974"
     CDS             5218..6666
                     /gene="thiI"
                     /locus_tag="ACHJ7J_000974"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO4999245.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGVDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(6720..7310)
                     /gene="yajL"
                     /locus_tag="ACHJ7J_000975"
     CDS             complement(6720..7310)
                     /gene="yajL"
                     /locus_tag="ACHJ7J_000975"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO4999246.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(7273..8184)
                     /gene="panE"
                     /locus_tag="ACHJ7J_000976"
     CDS             complement(7273..8184)
                     /gene="panE"
                     /locus_tag="ACHJ7J_000976"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO4999247.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEITQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8352..8843
                     /gene="yajQ"
                     /locus_tag="ACHJ7J_000977"
     CDS             8352..8843
                     /gene="yajQ"
                     /locus_tag="ACHJ7J_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO4999248.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(8971..10335)
                     /gene="yajR"
                     /locus_tag="ACHJ7J_000978"
     CDS             complement(8971..10335)
                     /gene="yajR"
                     /locus_tag="ACHJ7J_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4999249.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(10484..11374)
                     /gene="cyoE"
                     /locus_tag="ACHJ7J_000979"
     CDS             complement(10484..11374)
                     /gene="cyoE"
                     /locus_tag="ACHJ7J_000979"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO4999250.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11386..11715)
                     /gene="cyoD"
                     /locus_tag="ACHJ7J_000980"
     CDS             complement(11386..11715)
                     /gene="cyoD"
                     /locus_tag="ACHJ7J_000980"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO4999251.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(11715..12329)
                     /gene="cyoC"
                     /locus_tag="ACHJ7J_000981"
     CDS             complement(11715..12329)
                     /gene="cyoC"
                     /locus_tag="ACHJ7J_000981"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO4999252.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(12319..14310)
                     /gene="cyoB"
                     /locus_tag="ACHJ7J_000982"
     CDS             complement(12319..14310)
                     /gene="cyoB"
                     /locus_tag="ACHJ7J_000982"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO4999253.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(14332..15279)
                     /gene="cyoA"
                     /locus_tag="ACHJ7J_000983"
     CDS             complement(14332..15279)
                     /gene="cyoA"
                     /locus_tag="ACHJ7J_000983"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO4999254.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(15739..17214)
                     /gene="ampG"
                     /locus_tag="ACHJ7J_000984"
     CDS             complement(15739..17214)
                     /gene="ampG"
                     /locus_tag="ACHJ7J_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO4999255.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(17258..17836)
                     /gene="yajG"
                     /locus_tag="ACHJ7J_000985"
     CDS             complement(17258..17836)
                     /gene="yajG"
                     /locus_tag="ACHJ7J_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4999256.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            18141..18458
                     /gene="bolA"
                     /locus_tag="ACHJ7J_000986"
     CDS             18141..18458
                     /gene="bolA"
                     /locus_tag="ACHJ7J_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO4999257.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            18802..20100
                     /gene="tig"
                     /locus_tag="ACHJ7J_000987"
     CDS             18802..20100
                     /gene="tig"
                     /locus_tag="ACHJ7J_000987"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4999258.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            20346..20969
                     /gene="clpP"
                     /locus_tag="ACHJ7J_000988"
     CDS             20346..20969
                     /gene="clpP"
                     /locus_tag="ACHJ7J_000988"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO4999259.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            21095..22369
                     /gene="clpX"
                     /locus_tag="ACHJ7J_000989"
     CDS             21095..22369
                     /gene="clpX"
                     /locus_tag="ACHJ7J_000989"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO4999260.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            22557..24911
                     /gene="lon"
                     /locus_tag="ACHJ7J_000990"
     CDS             22557..24911
                     /gene="lon"
                     /locus_tag="ACHJ7J_000990"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO4999261.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            25102..25392
                     /gene="hupB"
                     /locus_tag="ACHJ7J_000991"
     CDS             25102..25392
                     /gene="hupB"
                     /locus_tag="ACHJ7J_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO4999262.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            25584..27455
                     /gene="ppiD"
                     /locus_tag="ACHJ7J_000992"
     CDS             25584..27455
                     /gene="ppiD"
                     /locus_tag="ACHJ7J_000992"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4999263.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            27606..27977
                     /gene="ybaV"
                     /locus_tag="ACHJ7J_000993"
     CDS             27606..27977
                     /gene="ybaV"
                     /locus_tag="ACHJ7J_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO4999264.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            28083..28481
                     /gene="fadM"
                     /locus_tag="ACHJ7J_000994"
     CDS             28083..28481
                     /gene="fadM"
                     /locus_tag="ACHJ7J_000994"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO4999265.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(28533..29228)
                     /gene="queC"
                     /locus_tag="ACHJ7J_000995"
     CDS             complement(28533..29228)
                     /gene="queC"
                     /locus_tag="ACHJ7J_000995"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO4999266.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(29293..30993)
                     /gene="ybaE"
                     /locus_tag="ACHJ7J_000996"
     CDS             complement(29293..30993)
                     /gene="ybaE"
                     /locus_tag="ACHJ7J_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO4999267.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKHGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGSFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKTVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            31093..31911
                     /gene="cof"
                     /locus_tag="ACHJ7J_000997"
     CDS             31093..31911
                     /gene="cof"
                     /locus_tag="ACHJ7J_000997"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO4999268.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            32064..32522
                     /gene="decR"
                     /locus_tag="ACHJ7J_000998"
     CDS             32064..32522
                     /gene="decR"
                     /locus_tag="ACHJ7J_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO4999269.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            32552..34324
                     /gene="mdlA"
                     /locus_tag="ACHJ7J_000999"
     CDS             32552..34324
                     /gene="mdlA"
                     /locus_tag="ACHJ7J_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4999270.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPAQENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            34317..36098
                     /gene="mdlB"
                     /locus_tag="ACHJ7J_001000"
     CDS             34317..36098
                     /gene="mdlB"
                     /locus_tag="ACHJ7J_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4999271.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDNNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVLQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            36279..36617
                     /gene="glnK"
                     /locus_tag="ACHJ7J_001001"
     CDS             36279..36617
                     /gene="glnK"
                     /locus_tag="ACHJ7J_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO4999272.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            36647..37933
                     /gene="amtB"
                     /locus_tag="ACHJ7J_001002"
     CDS             36647..37933
                     /gene="amtB"
                     /locus_tag="ACHJ7J_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO4999273.1"
                     /translation="MKIATIKTGLASLAMLPGLVIAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(37983..38843)
                     /gene="tesB"
                     /locus_tag="ACHJ7J_001003"
     CDS             complement(37983..38843)
                     /gene="tesB"
                     /locus_tag="ACHJ7J_001003"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO4999274.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            39061..39633
                     /gene="ybaY"
                     /locus_tag="ACHJ7J_001004"
     CDS             39061..39633
                     /gene="ybaY"
                     /locus_tag="ACHJ7J_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO4999275.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPANVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(39664..39975)
                     /locus_tag="ACHJ7J_001005"
     CDS             complement(39664..39975)
                     /locus_tag="ACHJ7J_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO4999276.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            40138..40234
                     /gene="ffs"
                     /locus_tag="ACHJ7J_001006"
     ncRNA           40138..40234
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHJ7J_001006"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            40354..40707
                     /gene="ybaA"
                     /locus_tag="ACHJ7J_001007"
     CDS             40354..40707
                     /gene="ybaA"
                     /locus_tag="ACHJ7J_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO4999277.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(40749..42299)
                     /gene="pdeB"
                     /locus_tag="ACHJ7J_001008"
     CDS             complement(40749..42299)
                     /gene="pdeB"
                     /locus_tag="ACHJ7J_001008"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO4999278.1"
                     /translation="MRTRHLVGLISGVLILLVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(42463..42933)
                     /gene="ylaC"
                     /locus_tag="ACHJ7J_001009"
     CDS             complement(42463..42933)
                     /gene="ylaC"
                     /locus_tag="ACHJ7J_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO4999279.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(43049..43600)
                     /gene="maa"
                     /locus_tag="ACHJ7J_001010"
     CDS             complement(43049..43600)
                     /gene="maa"
                     /locus_tag="ACHJ7J_001010"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO4999280.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(43772..43990)
                     /gene="hha"
                     /locus_tag="ACHJ7J_001011"
     CDS             complement(43772..43990)
                     /gene="hha"
                     /locus_tag="ACHJ7J_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO4999281.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(44016..44390)
                     /gene="tomB"
                     /locus_tag="ACHJ7J_001012"
     CDS             complement(44016..44390)
                     /gene="tomB"
                     /locus_tag="ACHJ7J_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO4999282.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(44936..48085)
                     /gene="acrB"
                     /locus_tag="ACHJ7J_001013"
     CDS             complement(44936..48085)
                     /gene="acrB"
                     /locus_tag="ACHJ7J_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO4999283.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(48108..49301)
                     /gene="acrA"
                     /locus_tag="ACHJ7J_001014"
     CDS             complement(48108..49301)
                     /gene="acrA"
                     /locus_tag="ACHJ7J_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO4999284.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            49443..50090
                     /gene="acrR"
                     /locus_tag="ACHJ7J_001015"
     CDS             49443..50090
                     /gene="acrR"
                     /locus_tag="ACHJ7J_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO4999285.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            50218..53580
                     /gene="mscK"
                     /locus_tag="ACHJ7J_001016"
     CDS             50218..53580
                     /gene="mscK"
                     /locus_tag="ACHJ7J_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO4999286.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            53641..53739
                     /locus_tag="ACHJ7J_001017"
                     /pseudo
     CDS             53641..53739
                     /locus_tag="ACHJ7J_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(53792..53953)
                     /gene="rsmS"
                     /locus_tag="ACHJ7J_001018"
     CDS             complement(53792..53953)
                     /gene="rsmS"
                     /locus_tag="ACHJ7J_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO4999287.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(53967..54494)
                     /gene="priC"
                     /locus_tag="ACHJ7J_001019"
     CDS             complement(53967..54494)
                     /gene="priC"
                     /locus_tag="ACHJ7J_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO4999288.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            54564..54941
                     /gene="ybaN"
                     /locus_tag="ACHJ7J_001020"
     CDS             54564..54941
                     /gene="ybaN"
                     /locus_tag="ACHJ7J_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO4999289.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            55094..55645
                     /gene="apt"
                     /locus_tag="ACHJ7J_001021"
     CDS             55094..55645
                     /gene="apt"
                     /locus_tag="ACHJ7J_001021"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO4999290.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            55774..57705
                     /gene="dnaX"
                     /locus_tag="ACHJ7J_001022"
     CDS             55774..57705
                     /gene="dnaX"
                     /locus_tag="ACHJ7J_001022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO4999291.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKDAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    57038..57102
                     /gene="dnaX"
                     /locus_tag="ACHJ7J_001022"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            57758..58087
                     /gene="ybaB"
                     /locus_tag="ACHJ7J_001023"
     CDS             57758..58087
                     /gene="ybaB"
                     /locus_tag="ACHJ7J_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO4999292.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            58087..58692
                     /gene="recR"
                     /locus_tag="ACHJ7J_001024"
     CDS             58087..58692
                     /gene="recR"
                     /locus_tag="ACHJ7J_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO4999293.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            58802..60676
                     /gene="htpG"
                     /locus_tag="ACHJ7J_001025"
     CDS             58802..60676
                     /gene="htpG"
                     /locus_tag="ACHJ7J_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO4999294.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            60857..61501
                     /gene="adk"
                     /locus_tag="ACHJ7J_001026"
     CDS             60857..61501
                     /gene="adk"
                     /locus_tag="ACHJ7J_001026"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO4999295.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            61633..62595
                     /gene="hemH"
                     /locus_tag="ACHJ7J_001027"
     CDS             61633..62595
                     /gene="hemH"
                     /locus_tag="ACHJ7J_001027"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO4999296.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(62592..63551)
                     /gene="aes"
                     /locus_tag="ACHJ7J_001028"
     CDS             complement(62592..63551)
                     /gene="aes"
                     /locus_tag="ACHJ7J_001028"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO4999297.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            63703..65007
                     /gene="gsk"
                     /locus_tag="ACHJ7J_001029"
     CDS             63703..65007
                     /gene="gsk"
                     /locus_tag="ACHJ7J_001029"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO4999298.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(65137..66813)
                     /gene="ybaL"
                     /locus_tag="ACHJ7J_001030"
     CDS             complement(65137..66813)
                     /gene="ybaL"
                     /locus_tag="ACHJ7J_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO4999299.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSHRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT        16654 a        16936 c        17590 g        15710 t
ORIGIN      
        1 cagtgcaatt catatttgtc aggtcgcatt tcttaaatct acaattgtta aaattagcat
       61 ttgtacaatt cgcatttgta aacttgacgc catcaaaata agtatgttcg aagttgacat
      121 ttgagcagtt acagtttccc atatctatac cgaaatcttt atcggcatct ttattgggat
      181 ctttaccgga aatattgctg tgtgctagat atagaccgct gaaatcgtaa tgtccattgt
      241 catccttttc tattgggagt gaataagtct ttactacctc gtcaacaata gagatagttc
      301 cacgcatcat cagattttct ttgtctatat aaaaaataat gttatgtgtg atggtactgt
      361 tgtatggctc tggaaatgag gatgcaattt tattccaaaa acaacaaagc gtggtctgct
      421 tttgtttggg cgagaggcta tcaaaatcca gctttaaata tgagtctaaa tctgacgttg
      481 catctatatt aaagttggga tctggagtta acgctgaatt ataatttaga actgacaatg
      541 tattgatgta tttatgaatc gatttaaatg ccttttgttt atcactgtcc agaaaaaaat
      601 cacgaatttt gttccacagc ctttcaataa gattttgatt taaatcttgc ttcattgagt
      661 ttcttaaatc tgcaaccggt acatcagtaa acagtatcgg ttgtttggtg atggttttca
      721 tcatgattgt aatcctctta cattgactaa ataagacatt tttaggggat attatcggga
      781 gaaactatca aaaatcgctc aaagcgatga agatgatgaa tagctgtaaa tttaatcacg
      841 aagaatttct cttgcagagg tggattatgg ttacagaagg tgatgggagg tacgttgtcc
      901 ccatcatcgc agacgatggg gaattcagca gattaaatgg gcgaaaagcg aaatttactt
      961 aaatcctacg accggatgcg gtttatacgg cgtttccagt tcggcaatct gttctggctt
     1021 caaagtgata tccaccgcgt tcagtagctc atcaagctgt tcttcccgcg atgtaccgat
     1081 aatcggtgcg gcaatgcccg gtttacttaa caaccaggcc agcgcaactt gtgctcgtgt
     1141 cgcttccagt tcttcactga cgcccgttaa ccgctccgca atttgcgcgt cattttcatc
     1201 gctttcttta tagagatttt tccccacctc atcagacacc aggcgtgcgg tagtttctcc
     1261 ccacggacgc gtcagtcgcc cccgcgccag cggactccac ggaatgaccg ccacgccttc
     1321 ctgataacac agcggcagca tctcgcgctc ttcttcgcga taaatcagat tgtagtgatc
     1381 ctgcatactg acaaactgcg cccagccgtg ctgtttttgt agttccagtg cctgagcaaa
     1441 ctgcgaagcg tgcatggatg acgcgccgat ataacgcgct ttcccggctt ttaccacgtc
     1501 gttgagggct tccagcgtct cttcgatcgg cgtgttgtaa tcccagcgat gaatttgcag
     1561 gatatcgaca taatccatgt cgagacgtcg caggctgtcg tcgatagagc gcaaaatttg
     1621 tgcacgggat aatccttccg gtaaatcacc aacgcgatgg aacactttgg tggcaacgac
     1681 cacgtcttca cgacgggaga aatcccgcag tgcgcgaccg acgatctctt cgctgctgcc
     1741 atcggaatag ctattggcgg tatcaaagaa atttatgccg ccttccagtg cgcgtttaat
     1801 tatgggacgg ctgctctctt ccggcagtgt ccatgcgtga ttaccacgat ctggctcgcc
     1861 aaaggtcata cagccgaggc aaagtcggga aacgcgaagg tcggtttttc ctaaggggtt
     1921 gtattgcatg ctgccactcc tgccatgaat aatttcttaa gcatagcagg agtggagtag
     1981 ggattatgcc agccaggcct tgattttggc ttccataccg gcggcatcga ggccgagttc
     2041 ggcgcgcatt tcttcctgag ttccttgtgg aataaagaag tccggcaggc caatgttcag
     2101 cacgggtact ggtttacgat gggccatcag cacttcgttc actccgctgc ctgcaccgcc
     2161 cataatggcg ttttcttcta cggtgaccag cgcttcatgg ctggcggcca tttccagaat
     2221 taacgcttca tcaagcggtt tcacaaaacg catatcgacc agcgtagcgt tcagcgattc
     2281 agcgactttc gctgcttctg gcatcagcgt accaaagtta aggatcgcca gtttctcgcc
     2341 acgacgcttc acaatgcctt tgccaattgg tagtttttcc agcggcgtca gttccacgcc
     2401 aaccgcgttg ccgcgcgggt agcgcaccgc ggacgggcca tcgttatagt gatagccggt
     2461 atagagcatc aggcgacatt cgttttcatc gctcggagtc ataatgacca tttccggtat
     2521 gcagcgcagg taagagagat caaaagcgcc ctgatgggtt tgaccgtcag caccaacaat
     2581 gcccgcacgg tcgatggcga acaggaccgg aagcttttga atcgccacgt catgcaacac
     2641 ctgatcatag gcgcgttgca ggaaagtgga gtaaatcgcg acaatgggtt tgtacccacc
     2701 aatcgccaga ccggcggcaa aggtcaccgc gtgttgctcg gcaattgcca catcgaagta
     2761 gcgatccggg aatttacgag aaaactcgac cataccggaa ccttcacgca tcgccggagt
     2821 aatcgccatc agcttgttgt ctttcgctgc cgtttcgcac aaccagtcgc caaagatttt
     2881 tgaatagctt ggtaaaccac cgctactttt cggcaaacaa ccgctggagg gatcaaattt
     2941 aggcacggcg tggaaggtga ttgggtcttt ttctgccggt tcataaccac gacctttttt
     3001 ggtcatgata tgcaggaact gcgggccttt caggtcgcgc atgttcttaa gcgtggtgat
     3061 aagccccagc acatcgtgac cgtcaaccgg gccgatgtag ttaaagccca gctcttcaaa
     3121 caacgtgcca ggcactacca tgcctttaat atgttcttcg gtacgtttga gcagctcttt
     3181 aattggcgga acgccagaga aaactttttt cccgccttcg cgcagtgaag agtaaagctt
     3241 accggaaagc agctgtgcca gatggttatt gagcgcgcca acattttcgg aaatcgacat
     3301 ttcattgtcg ttgagaacca ccagcatatc aggacggata tcgcccgcgt gattcatcgc
     3361 ttcaaacgcc atgccagcgg taatagcgcc atcgccaatg acacagacgg tacggcgatt
     3421 tttgccttct ttctcggcag caaccgcaat accaattccg gcactgatgg aggttgatga
     3481 atgcccgacg cttaatacgt catattcgct ttcgccgcgc cacgggaacg ggtgtagacc
     3541 gcctttctga cggatggtgc cgattttgtc gcggcgtccg gtcaaaattt tatgcggata
     3601 agcctgatgc cccacatccc aaatcaattg gtcaaacggg gtgttataga catagtgcag
     3661 cgccacggtc agttcgaccg tgcccagccc ggaggcgaag tgcccgctgg aacggctcac
     3721 gctgtcgagt aaatagcggc gcagttcgtc gcagagtttc ggtaaactct cttttggcaa
     3781 cagtcgtaac tcctgggtgg agtcgaccag tgccagggtc gggtatttgg caatatcaaa
     3841 actcatcagg ggcctattaa tacttattgt ttatttatta cgctggatga tgtagtccgc
     3901 tagcgcttcc agtgccgaag tatcgatcga ctgttcagcc agttgtttca gcgactggcg
     3961 ggcatcgtcg atcagatcct gggctttctt ccgggcttgc tcaagaccca ggagtgcagg
     4021 gtaggtactt ttgccaagct gctggtcggc accctgacgt tttcccaacg ttgcagtatc
     4081 tcccaccaca tccaggatgt catcctgaac ctggaaggca aggccgatgc tctctgcgta
     4141 cttgtcgagt actggcagag cacgacgccc tttatctccg gcgcttaatg caccaaggcg
     4201 aacggcggcg cgaatcaatg cgccggtttt atgacgatga atacgctcga gtgcgtccag
     4261 aggtacatgt ttgccttccg cgtctaaatc taatgcctga ccaccgcaca ttccggcaat
     4321 accgctggcg ctcgccagtt cagaaatcat cgaaattctg tcgcgatccg acacttccgg
     4381 catatcggca tcgcttagaa tcgagaacgc cagcgtttgt aaagcatcgc cagcgagaat
     4441 cgcgttcgct tcgccaaact tcacatggca ggtcggcaaa ccgcgacgca gatcgtcatc
     4501 atccatcgcc ggtaaatcat catgaattaa tgagtaagcg tggatacact caacggcggc
     4561 agcgggtgcg tccagcgtgt ttgtgctaac gccgaacata tgaccggtgg cataaaccag
     4621 aaaaggtcgc aggcgcttac cacctaataa tgcgccatac tgcatggttt cgaccacggg
     4681 agtgttctga aagggcagtg gggcgataaa acggctcagc gcctggttgg cctgcttaac
     4741 gcaggcttcg agttgctgcg gaaagtccat tactcattgt ccggtgtaaa aggggttaga
     4801 gaggcgtctt cattgtcaga cagcagaatt tgtacgcgct gttcggcttg ttgtaatttg
     4861 gcctgcccct gacgtgccag ctgcacgccg cgttcgaact cgttcagcgc ctcttccagc
     4921 ggcaggtcgc cactttccag acgggttaca atctgttcca gctcgctcag cgccttttca
     4981 aagctggcgg gcgcctcatt tttcttcggc ataatgaatg tctgactctc aatatttttc
     5041 gccccgtcat ggtaacggac tcagggcaaa tagcaaataa cgcgcaatgg taaggtgatg
     5101 tgcgcagcaa agcgatgtta gtggtatact tccgcgcctg gatgcagccg caggtgtggg
     5161 ctgctgtatt tttccctata caagtcgctt aaggcttgcc aacgaaccat tgccgccatg
     5221 aagtttatca ttaaattgtt cccggaaatc accatcaaaa gccaatctgt gcgcttgcgc
     5281 tttataaaaa tccttaccgg gaacattcgt aacgttttaa agcactatga tgagacgctc
     5341 gctgtcgtcc gccactggga taacatcgaa gttcgcgcga aagatgaaaa ccagcgtctg
     5401 actattcgcg acgccctgac ccgtattcct ggtatccacc atattctcga agtcgaagac
     5461 gtgccgttta ccgacatgca cgatattttc gagaaagcgt tggttcagta tcgcgatcag
     5521 ctcgaaggga aaaccttctg cgtacgcgtg aagcgccgtg gcaaacatga ttttagctcg
     5581 attgatgtgg aacgttacgt cggcggcggt ttaaatcagc atattgaatc cgcgcgcgtg
     5641 aagctgacca atccggatgt gactgtccat ctggaagtgg aagacgatcg tctcctgctg
     5701 attaaaggcc gctacgaagg tattggcggt ttcccgatcg gcacccagga agatgtgctg
     5761 tcgctcattt ccggtggttt cgactccggt gtttccagtt atatgttgat gcgtcgcggc
     5821 tgccgcgtgc attactgctt ctttaacctc ggcggcgcgg cgcatgaaat tggcgtacgt
     5881 caggtggcgc attatctgtg gaaccgcttt ggcagctccc accgcgtgcg ttttgtcgct
     5941 attaatttcg agccggtcgt cggggaaatt ctcgagaaaa tcgacgacgg tcagatgggc
     6001 gttatcctca aacgtatgat ggtgcgtgcc gcgtctaaag tggctgaacg ttacggcgta
     6061 caggcgctgg tcaccggcga agcgctcggc caggtgtcca gtcagacgct gaccaacctg
     6121 cgcctgattg ataacgtctc tgatacgctg atcctgcgtc cgctgatctc ttacgacaaa
     6181 gagcacatca tcaacctggc tcgccagatt ggcaccgaag actttgctcg cacgatgccg
     6241 gaatactgcg gtgtgatctc caaaagcccg acggtgaaag cggttaaatc gaagattgaa
     6301 gcggaagaag agaagttcga cttcagcatt ctcgataaag tggttgagga agcgaataac
     6361 gttgatatcc gcgaaatcgc ccagcagacc gagcaggaag tggtggaagt ggaaactgtc
     6421 aatggcttcg gcccgaatga cgtcatcctc gatatccgtt ctatcgatga acaagaagat
     6481 aagccactga aagtcgaagg ggttgatgtg gtttctctgc cgttctataa actgagcacc
     6541 aaatttggcg atctcgacca gagtaaaacc tggctgttgt ggtgtgagcg cggggtgatg
     6601 agccgtctgc aggcgctcta tctgcgcgag cagggcttta acaatgtgaa ggtatatcgc
     6661 ccgtaatttg tggttgtaac gtcgcatctg gtcagatgcg acgcttaccg catccgacac
     6721 tactcgtaat aattataaat ccctgccgcc atcaccagtt gtgatgccac ttcatgggct
     6781 ttctcgcgcc caaccaacag gtcgataatt ttcagaccaa agtcgatagc tgtacccggc
     6841 ccctggctgg tcagcaattt tacccgtgca tcccagacga cgcgtttgtc ctgccattgt
     6901 tcggcgggaa ttttgtcttt cagcgttggg aagccggtca tattgccaat cgggaagata
     6961 tcgtgcggca ccagcacggt ggctggcgcg gcgcaaatag ccgcgacgat acgcccggaa
     7021 cggtggaact gtttaacggt ttcaaccagc agggtgctat cgcgaaaaca ctccgcgcct
     7081 ttaatgccac caggcagcac gatcacgtca tattcgccat cagccacttc gaccagcggc
     7141 gcatccgcca gcagcttaac gccgcgcgag caggtaatcg ccaggttgcc atcgctggct
     7201 acgctggcag tggtgacttt gataccgccg cgaaccaaga ggtcgatcgt ggtgacggct
     7261 tcagtctctt cactaccagg ggcgaggcaa accagtgccg atgcgctcat attcactctc
     7321 ctttcttttt accatttcaa acaggcgggt gttttccggt acggcaatcc catgcgcgcg
     7381 ggcgcggcgt aagagaaaac cattgatata gtcgatttcg gtgtggcgca gcgcgcggat
     7441 atcctgcaac atcgacgaga tattttccgc tgtggcatca atcacctgca tcacgtaatc
     7501 acgtaaatct tccgctgaag tatgatgccc ttcgcgttcg atcactgccg cgacttcttc
     7561 gcatatttgc gtaatttctt gcggatgatg acgtaattca ccgttcgggc aattccagat
     7621 ggcagtcagt ggattaatca cacagttgac tgccagcttg cgccacagct cggcgcgaat
     7681 attgttatgc caggcaacgt caggcaacac ggtttgcaaa atatccgcca gataactgta
     7741 atcgccgtcc tgttgccgtg ctggaccaat atgcgtgata ccgtttgcca catgaataat
     7801 gacattgccg tcgcggcggg cggcatgggt ggtggtgccc atcagtaatg gctgctgaat
     7861 gttttgcaat tcttcgatgg tgcccatgcc gttgtgaatt aacagtattg gcgtagttac
     7921 aggcagtgtg gacgcgaggc ttttgacggc atcggaaacc tgccatgctt tcagcgtcac
     7981 caggagcaga tcgctggtgg caagaaaatc gggatcgttg gcggtcagcg attcgttaaa
     8041 tatcgaacca tcagtctcaa ccagattcac actacaataa ggttgcggta cgcgcagcca
     8101 gccctgaact tcgtgaccct gtttacaaag tgctgtaagc cataattgcc ctaaggcacc
     8161 gcatcccaat acggtaattt tcattgttcc tcctcacccg caaccactcc gggtgttcaa
     8221 taaggctatc ccttaattgt gcatgctgtt gcgactatgc acaattaagg gatacgtcct
     8281 ggtgcaggac tgttggttat ttaactttgc aggtattatg cttcgcatca aaaatgaagg
     8341 gagaggaaaa gatgccatct ttcgatattg tctctgaagt tgatcttcag gaagcacgta
     8401 acgcggtcga taacgcgagc cgcgaagtgg agtcccgttt tgacttccgt aacgttgaag
     8461 cctcatttga gctgaacgac gccagcaaaa ccatcaaagt gttgagcgag tccgatttcc
     8521 aggtcaatca gttgctggat attttgcgtg ccaagctgct gaagcgcggc attgaaggca
     8581 gttcgctgga tgtaccggaa aatatcgttc atagcggtaa aacctggttt gtggaagcga
     8641 aactgaaaca gggcattgag agcgcgaccc agaagaaaat cgtcaagatg atcaaagaca
     8701 gcaaactgaa agtgcaggcg caaattcagg gcgatgagat ccgcgtgact ggcaaatctc
     8761 gtgatgattt gcaggctgtc atggcgatgg tgcgcggtgg cgatctgggt cagccgttcc
     8821 agttcaaaaa cttccgcgat taatcgcgac aacctgcgct ttgttcatgt cagatgcggc
     8881 atgaacgcct gattcggcct acaaagcttt gtaactcagg aacttagtag gcctgataag
     8941 cgtagtgcag caggcaattt cgcctcgctt ttatgcctgg cgaattgcct gttcaacctc
     9001 aaagcgattt gtcactttgc tgtcgatttt cacataagct gaatgttctt cttctgcaat
     9061 caacacttct ttgatgcctt cagtttctag caaacgcact tttaacgcct cgtttgcggc
     9121 aatgttcgcc gggatttcaa tgcgcaagct gctgacatac ggcggttctt tcatggtact
     9181 ggcgactgcc agccacactg cggccagcat tgcgccagcg agaaatacgc cctgaccgtc
     9241 aaacatgccg tcaatccagc cgcccagtga accgccaatc gccacgccaa gaaactggct
     9301 ggtggagtaa acacccatcg ccgtaccttt gtaacctgct ggcgactctt tactgataag
     9361 tgagggcagg agggcttcca tcaaattaaa cgccacaaag aaaagctgca cgccgaccac
     9421 cagttgccag aactgcgttt gcgcgttcca caacacaatt tccgcaacca cgatcaaccc
     9481 gacgcagaag acaaagactt gcttcatttt gcgcttaact tcagcgtaga taatgaaagg
     9541 cacgaccgag ccaaaggcga ttagcattgt cgccagatag accttccagt gttcagccgc
     9601 cgggaacccc gcatcagcca gttgcccagg cagagcaaca aaggtcgaca tcagcaaaat
     9661 atgcagacac ataatgccaa agttgagttt cagcagccgc ggttccgcca gcactttgct
     9721 gaaactgcct ttcaccattc cggactcacg attaagtacg tgagtgctac tgttgggcac
     9781 aacccaaatg gtcaacgcaa tgccggtcgt tgccagaata gcgatcatcc agaacagcgc
     9841 gtgcagccca agtttgtgag tgatgatcgg gccaagcacc atcgcaatgg caaaggtaat
     9901 gccaaagctc acgccgataa acgccattgc tttggtgcgg ttttgttcgc gcgtgagatc
     9961 ggaaagcagc gccataacgg cagcggcaat cgcaccggag ccttgtagcg cccgacccag
    10021 aataattccc cagatggagt cagagagcgc agcgataacg ctaccggcgg caaacactgc
    10081 cagcccaccg acaattaatg gtttgcgacc aatgcggtcg gaaagcaggc caaacggaat
    10141 ctgaaaaacg gcctgagtca gaccataaat accaatggca ataccgatta atgcttcgct
    10201 ggcaccttgc agagccatgc cgtacgtggt cagaaccggc agaaccatga acatgcccag
    10261 catgcgcaac gagaatacgg tccctaaacc ccaggtcgcg cgccgctcac ctggcgtcat
    10321 tttataatcg ttcattacca cctctgtttt aaattcgcga ctagtgtaaa gcggcaaacg
    10381 aatggggtaa acatgcggat tttttagtaa atatttcgag agggatatgt ttctaatgct
    10441 aagaaaaaag gtgccgtagc acctttttaa tagagaggtt ttgttaccac acagcagcca
    10501 gcagcgtatg cgagtccggt accataaaat caacggacat catcaccgag agggcagtga
    10561 tggcgatgat agagaagccg aacagcttgc gcgcccagat tctgtcatca gcaactttat
    10621 aaccgcgcag agccatacct aaccaccaga cgctaaccgc cgcggcgacc accagatatt
    10681 tatacccagc gtaaccgcca agagagagca tcagcgtggc aacggcaaag gcgatgatat
    10741 acagcgtgat gtgattcttc gccaccgaaa tgccttttac cactggcaat accggaatgt
    10801 ttgccgcctg gtaatcctta aagcggaaaa tggcgatggc ataggagtga ggcatctgcc
    10861 acaggctgaa gatagccagc aggatcgctg cgccgctatc gaactcgccg gttactgcac
    10921 agtagccaat caccggcggc gcagcgccgg agagcgaacc aatcaatgtg ccgtagactg
    10981 agtggcgttt catgtacagg ctataaacgc cgacataaac cacaaagccc atcaccccca
    11041 gccagcaggc cagcggattc gcgccaaacc acagcagcat aaagccagca atacccagca
    11101 aggtggcgta caccagcgag acagcaggag agatcaggcc tttaaccagc acccgattct
    11161 tcgtcctttc catctttctg tcgatatccc tgtcgatgta gttgttaaac acacaacccg
    11221 acgccacaac cagtgacacc ccaaccagcg tgtagataaa caggggataa tcaatgctgc
    11281 cctttgaggc cagcaggaat cccccaatca ccgagatcag gttgccaaag atgatgcctg
    11341 gtttcgttac ttgcaggtat tgcttaaaca tcataaccgc cgctcttagt gcatcatcat
    11401 gttgtagttg aggttccaca taatccagat ggagcctaca accaggatag cgatgattag
    11461 cacggtgaag acaaacgccg tcatgttcca gccttcatct gatttggtat tcatgtgcag
    11521 gaagcacacc agatgcacca gaacctgtac cactgccatt gccaggattg ttcccagaat
    11581 tacggccgga gaggcagccc ctgtcatcac catccagaac ggaatcaccg tcagaatgat
    11641 cgacaggata aagcctgtca tgtaggtttt tacgctgcca tgggacgcgc cgctgtgatc
    11701 ggtagaatga ctcattacat cgcccccatc agataaacaa cagtgaacac acagatccaa
    11761 accacatcca ggaagtgcca gaacaggctc aggcacatga tgcgggtacg gttagtgctg
    11821 gtcaggccgc gacgagcgat ttgtaccatc agcaccgcca tccagataag accggaagtg
    11881 acgtgcagac cgtgcgtgcc gaccagcgca aagaacgctg acaggaagcc gctgcgatcc
    11941 ggacccatgc cgttaacaat caggtgatgg aattcgtaga tttccatccc gataaatccg
    12001 gcgccaaaca accaggtcaa cgccagccag gagataacct ggcttttgtt gtttttgtac
    12061 atggcgatag ccgccatgcc gtaggtgatg gagctgaaca acagcaagaa agtttcaacc
    12121 agaacgaacg gcagttcgaa aatgtcctta cctgtcgggc cgcctgcggt gccgttcacc
    12181 agaacggcat aggtagcaaa caagatagag aacagaatgc agtcgctcat caggtagatc
    12241 caaaatccga agattttggt tccgcctgca tcgtggtgcc cgtgttcgtg cgcgtgggca
    12301 gtcgcgtgcg tcaaagtatc agttgccatt tttcagccct gccttagtaa tctcatcgaa
    12361 atgctggttt tccagttttt cgatttctgc caccggcacg tagtaatcca cgtcctcgtc
    12421 gaagcttttc acgatccagg tgatgatcat gcctgcgaag ccaacaatcg ccagccacca
    12481 gatatgccag atcatggcga aaccgaagat ggtggagaaa gctgcaatga cgatacctgc
    12541 accgctgttt ttcggcatat gaatttcttc atagtggtca ggctttttat acgcttcgcc
    12601 tttctctttc atttcccaga atgcatcacg ctcgtgaacg tgcggcacaa cggcaaagtt
    12661 atagaacgga ggcggggaag aggttgccca ctccagcgta cggccacccc acgggtcgcc
    12721 agtcaggtca cggttctggt cgcggtcacg aatagaaacg tacatctgaa taacgaggca
    12781 gagaatacct agcgcaatca gtactgcacc gctggctgca atcatcagca tggtgtggaa
    12841 ctgcgggtca atctgctggc tcaaacgacg ggtcatgccc atgaagccca gcgcgtacag
    12901 tggcataaag gcaacgaaga agccgatgat ccagaaccag aacgcgcgtt taccccaggt
    12961 ttcgttcagt ttgaaaccga acgctttagg ccaccagtag gtcatccctg cgaagcagcc
    13021 gaagaccacg ccgccgatga tcacgttatg gaagtgcgca atcaggaaca ggctgttatg
    13081 tagaacgaag tccgcgcccg gtactgccag cagcacgcca gtcataccgc ccaccgagaa
    13141 ggtgacgata aaaccgatag tccacagcat cgcagaatgg aacacgatgc ggccctgata
    13201 catggtgaac agccagttga agatcttcac accggtcggg atggcgataa tcattgtggt
    13261 gataccaaag aaggcgttta cgttcgcgcc cgcacccatc gtaaagaagt ggtgcagcca
    13321 aacgatgaac gacagcacgg tgatacagac ggttgcccat accagcgagg tataaccaaa
    13381 cagacgttta cgcgagaagg ttgccgcaat ttcggagaac acaccgaaaa caggcaggat
    13441 caggatgtaa acttccgggt ggccccaggc ccaaatcagg ttgatgtaca tcatcatgtt
    13501 gccacccata tcgttggtaa agaaatgggt gcccagatag cgatccaggg tcaacaacgc
    13561 gacggtaacc gtcagaattg ggaaggaagc aataatcagt acgttcgcgc acagtgatgc
    13621 ccaggtaaat actggcatct tgaacatggt catgcccggt gcgcgcatct tcagaatggt
    13681 aacgaagaag ttgataccgg taagcgtcgt acctataccg gatagctgga gactccatat
    13741 ccagtaatcg acaccgactc ccggactgta ctctattccc gatagcggtg gataggccag
    13801 ccagccggtc tgcgcaaatt cgcccacgcc gagagaaacg ttaaccagaa tcacaccaac
    13861 aacggtaaac cagaagctta agttgttgag gaacgggaac gcaacgtcac gcgcgccaat
    13921 ctgcagcgga accaccaggt tcatcagacc gataacgaaa ggcatcgcca cgaagaagat
    13981 cataatcacg ccgtgcgcgg tgaagatctg atcgtagtgg tgaggtggca ggaagcccgc
    14041 ttcgcccgcc gaggcaagag cctgctggct acgcatcata atcgcgtcag caaaaccacg
    14101 cagcaacatc acaatcgcca cgatgatata catgataccg aggcgtttat ggtcgacgga
    14161 ggtcagccac tctttccaca ggtaggtcca cttaccgaag taagtgatca ggccaacgag
    14221 cgccagacct cccaaaataa tgccagcgat cgtaaccatg acgataggtt catggaacgg
    14281 gactgcatca agtgataatt ttccgaacat ctttattctt cctcaacccc tttaatgggc
    14341 ggattccgcg tggctcatgt ccatgccttc cataccttcg tgtgcgctgt gctcaccttc
    14401 tggctgggtc atgtccatgc tcttaccgtg agccataaac ttgttaatta catcggcaaa
    14461 caagtctggt ttcacgttgg agaaatattc cacctggttg tattcgctag gcgcggccag
    14521 tttttcgaac gctgccatgt cagacatgga gttcggcgac tgtttcgctt ttgcgaccca
    14581 ctggtcgaat gcggcgcgat ccggtgttgc aatagctttg aacttcatgc ctgagaagcc
    14641 cgggccgctg tagctggcgg agataccgtc ataagtgccg ggttcgttgg cgatcagatg
    14701 caggcgagtc tgcataccgg ccatggcata aatctggcta cccagacgcg gaatgaagaa
    14761 ggagttcatc acggagttgg aggtcacttt gaagtacacc ggagtgttcg ccgggaaagc
    14821 gatttcattc acggtagcaa tgccctgttc cgggtagatg aagaaccatt tccagtccat
    14881 ggaaaccact tcgatggtaa tgggcttctc gtcgtgtgcc agcggcttgc taggctcaag
    14941 agcgtgagtg gttttccagg tcagtaccgc aaggaagatg atgattaaga taggtaccgt
    15001 ccagaccaca gcttccactt tattggagtg tgaccagttc gggctgtact tagcatcttt
    15061 attgctcgca cggtacttcc aggcgaaacc aacagccatc aagattgcgg gaataacgac
    15121 aatcaacatc aggccaaatg ccgtcagtat cagtgaacgt tgctccagac caatctgtcc
    15181 tttgggatct aacagcgcag aattacagcc actgagcaat acagtgcctg caaataatga
    15241 caaccatccc aaacttttat tgtatttcct gagtctcatt taacgacctc aattccacgg
    15301 gatctggtgg cgtttaaagt gtgtgggcat tttacgggaa ggttacatta ctgtaaacat
    15361 gattaaattc ctgttacttg gatttggctg tcaggtcaca tatgttgcaa aacatatcag
    15421 gttgatttta agaagctgtt gcaaaagggc gttataacaa aaggggagtg cagaaggagt
    15481 ctgggcgggc aattggtata accaatgcga aataaaacaa ttatttaaca aataattatc
    15541 attggagtga caacttggtg gtgagaaaat aaagactcat tagctgaatc gtgtaagaaa
    15601 aatagaattt ataaatggcg ccaataattt ccaaacgcta agccgcacaa aagaacaaat
    15661 attaataagc gatgctgatg tttatttagc cgtaataatt acggcgagtg attactacac
    15721 ctaaataata tttacagatt acgtcagatg cgtttttcgt agcgccagat aatccagcaa
    15781 accaccaagc acgacgccag aaagggcgac taaaaccccg acttccagca gagcaggcag
    15841 gaaagagaag tgcgtcaaat ccagcgcgtc catcgtcaac agcagtaacc acacggccaa
    15901 caggctgacg cccgccgcca gtgtccacat ggcaaaggca taacctgccg gatatgcggt
    15961 acgggagata aagttgtcat ttactcgtgt atattcaagc gtctggcggc aaaccagcag
    16021 caaaataagc cctggtacgg cagcggcgac ggagaataga tagaatgtcg accagccgtg
    16081 tgcttcaaca aaccaacccg ccacggggcc gacataaact cgccctacag cagaaagcgc
    16141 tgagagcagg gcaaattgag tagcggaaaa tgacttatta catagcgtca ttaacagcgc
    16201 gacaaaggct gatgtgccca tcccgccaca gaggttttcg aaaaagacgg ctgcgcccat
    16261 gctgtagaga tgcttatcag taatcgacag cagccagtaa ccagcgttag acgcaccttg
    16321 taaaatgccg aaaatcagca gtgcccggaa cagtgacagg cgctgcatca aaatcccacc
    16381 gtacaacgcg ccaacaatgg tcgctagtaa gccaagcgtt ttgtttacca cgcctacttc
    16441 acccgcatca aacccgacgc cgcgaatcaa aaacgtggtt gtcaggctca tggcgaatgc
    16501 gtcgcccagc ttatacagca cgataagaag caaaataagc caggcattat tgcgaccaaa
    16561 gaaatctcgc agaggtgcaa caaccgcttg ttccagcgtt tttggcacag gaatggtgtc
    16621 ggttggttct ggtgcaagca acgtcgcgat aatacagggg atcaacagcg ccgccatcaa
    16681 ccagtacatg ccctgccagc ccagccattt atctgccagc cacagggcta ggccgccgga
    16741 aaccagcatc cctaaacggt agcccagaac gctgattgcc gcgcccgcac cacgttcttc
    16801 tgccgggagc acatcggttt tccacgcatc gaagacaata tcctgggagg cagagcaaaa
    16861 agcgatcacc accgccagcg ccgccatcca gcggagttgg gtgcctggtt cgagaaaacc
    16921 catcgccgca atggcgacta ataacaggat ttgcgtggcg agcagccaac cgcgccgccg
    16981 cccaaaaaat gggggcgtgt agcggtccat cagcggtgac cagaggaatt taaaaacgta
    17041 agcctggcct accagagaga agaaaccaat ggttttgaga tcgatattct cgaccgtcat
    17101 ccaggcctgt aaggtgccgg atgtcagggc gagcggtagc ccggaagcaa aacccaggat
    17161 cagcaatatg gctgaacgcg gctgttgaaa aatacgtaaa tattgactgg acatgggcgt
    17221 ctacaggccc ggcttgcacc gggccagagg gcagaaatta acgcgcgttc tgcttgatga
    17281 attcgtgaat actggtgtcc tgagacatat cagcgatggt atcggtcagc acactgttaa
    17341 ccgcatcggc gatatttttg ttggaggcct ggaacgcacc ttcaacgttg tagctggcac
    17401 gatagttttt ggtcattttg ttgccattct gcgcggtagc gatgatggcg atatccgctt
    17461 tggtagcgat gttgtagcgc acgttgccct gggacacgtc agcatacagt tggctaacga
    17521 tgatttgcag attaaccggg ccattcggac caaccatgta accacgcgcg gtcatctgtt
    17581 tttccagcac ttcttgcagc aggaaacgca gatcgcggga ggcggtcagg gtaacgattt
    17641 gattatcgcg ggtgactttt gccagcgcct gatcggtacg ctgatcggca ccattaatgc
    17701 ttacggtgac gcccatcagg cttggatcct gctgtggcag tgtaatcgtc ggggaaactt
    17761 caatagttgt tggcggtttt gcgcatcctg caagcataaa cagagcaact aacgggaaga
    17821 ggattttttt gaacatgttc gggctctcag agactcttaa gcgtgtttgg taaaaattcc
    17881 cgccatcata acattgccaa cggcgagggg aagtaggtaa ggcatgtaaa ttcatcatgt
    17941 tgacgaaata atcgcccctg gtaaaagaaa cgctgatgcg aggcctgtgt ttcaatcttt
    18001 aaatcagtaa acttcatacg cttgacggaa aaaccaggac gaaacctaaa tatttgttgt
    18061 taagctgcaa tggaaacggt aaaagcggct agtatttaaa gggatggatg acatctcagc
    18121 gttgtcggag gagatatttc atgatgatac gtgagcggat agaagaaaaa ttaagggcgg
    18181 cgttccaacc cgtattcctc gaagtagtgg atgaaagcta tcgtcacaat gtcccagccg
    18241 gctctgaaag ccattttaaa gttgtgctgg tcagcgatcg ttttacgggt gaacgttttc
    18301 tgaatcgtca tcgaatgatt tacagtactt tagcggagga actctctact accgttcatg
    18361 cgctggctct gcatacttac actattaagg agtgggaagg gttgcaggac accgtctttg
    18421 cctctcctcc ctgtcgtgga gcaggaagca tcgcgtaaaa acgcatttgc aactgtcggc
    18481 gcttttccag tatgttgcta aagattttat gaaaaacggc ctgcgggccg ttttgttttg
    18541 tctggatttt gcgctttttg cccagcattc agacgaaaat tgcccgggaa ttgtgaaaaa
    18601 atacgcgaca gcgcgcaata accgttctcg actcataaaa gtgatgccgc tataatgccg
    18661 cgtcctattt gaatgctttc gggatgattc tggtaacagg gaatgtgatt gattataaga
    18721 acatcccggt tccgcgaagc caacaacctg tgcttgcggg gtaagagttg accgagcact
    18781 gtgatttttt gaggtaacaa gatgcaagtt tcagttgaaa ccactcaagg ccttggccgc
    18841 cgtgtaacga ttactatcgc tgctgacagc atcgagaccg ctgttaaaag cgagctggtc
    18901 aacgttgcga aaaaagtacg tattgacggc ttccgcaaag gcaaagtgcc aatgaatatc
    18961 gttgctcagc gttatggcgc gtctgtacgc caggacgttc tgggtgacct gatgagccgt
    19021 aacttcattg acgccatcat taaagaaaaa atcaatccgg ctggcgcacc gacttatgtt
    19081 ccgggcgaat acaagctggg tgaagacttc acctactctg tagagtttga agtttatccg
    19141 gaagttgaac tgcagggtct ggaagcgatc gaagttgaaa aaccgatcgt tgaagtgacc
    19201 gacgctgacg ttgacggcat gctggatact ctgcgtaaac agcaggcgac ctggaaagaa
    19261 aaagacggcg ctgttgaagc agaagaccgc gtaaccatcg acttcaccgg ttctgtagac
    19321 ggcgaagagt tcgaaggcgg taaagcgtct gatttcgtac tggcgatggg ccagggtcgt
    19381 atgatcccgg gctttgaaga cggtatcaaa ggccacaaag ctggcgaaga gttcaccatc
    19441 gacgtgacct tcccggaaga ataccacgca gaaaacctga aaggtaaagc agcgaaattc
    19501 gctatcaacc tgaagaaagt tgaagagcgt gaactgccgg aactgactgc agaattcatc
    19561 aaacgtttcg gcgttgaaga tggttccgta gaaggtctgc gcgctgaagt gcgtaaaaac
    19621 atggagcgcg agctgaagag cgccatccgt aaccgcgtta agtctcaggc gatcgaaggt
    19681 ctggtaaaag ctaacgacat cgacgtaccg gctgcgctga ttgacagcga aatcgacgtt
    19741 ctgcgtcgcc aggctgcaca gcgtttcggt ggcaacgaaa aacaagctct ggaactgccg
    19801 cgcgaactgt tcgaagaaca ggctaaacgc cgcgtagttg ttggcctgct gctgggcgaa
    19861 gttatccgca ccaacgagct gaaagctgac gaagagcgcg tgaaaggcct gatcgaagag
    19921 atggcttctg cgtacgaaga tccgaaagaa gttatcgagt tctacagcaa aaacaaagaa
    19981 ctgatggaca acatgcgcaa tgttgctctg gaagaacagg ctgttgaagc tgtactggcg
    20041 aaagcgaaag tgactgaaaa agaaaccact ttcaacgagc tgatgaacca gcaggcgtaa
    20101 tttacgcagc ataacgcgct aaattcgcac aaaggcccgt caccgccagg tggtgggctt
    20161 ttttttgtca tgaattttgc atggaaccgt gcgaaaagcc tctttcggtg ttagcgtaac
    20221 aacaaaagat tgttatgctt gaaatatggt gatgccgtac ccataacaca gggactagct
    20281 gataatccgt ccataaggtt acaatcggta cagcaggttt tttcaatttt atccaggaga
    20341 cggaaatgtc atacagcggc gaacgagata actttgcacc ccatatggcg ctggtgccga
    20401 tggtcattga acagacctca cgaggtgagc gctcttttga tatctattct cgtctactta
    20461 aggaacgcgt catttttcta actggccagg ttgaagacca catggctaac ctgattgtgg
    20521 cgcagatgct gttcctggaa gcagaaaacc cagaaaaaga tatctatctc tacattaact
    20581 ctccaggcgg ggtgattact gccgggatgt ctatctatga caccatgcag tttatcaagc
    20641 ctgatgtcag caccatctgt atgggccagg cggcctcgat gggcgctttc ttgctgaccg
    20701 caggggcaaa aggtaaacgt ttttgcctgc cgaattcgcg cgtgatgatt caccaaccgt
    20761 tgggcggcta ccagggccag gcgaccgata tcgaaattca tgcccgtgaa attctgaaag
    20821 ttaaagggcg tatgaatgaa cttatggcgc ttcatacggg tcaatcatta gaacagattg
    20881 aacgtgatac cgagcgcgat cgcttccttt ccgcccctga agcggtggaa tacggtctgg
    20941 tcgattcgat tctgacccat cgtaattgat gccagaggcg caactgtgcc gctatactta
    21001 tccagggcgg cacaacgctg taagcggctt gcgcctgaga atggcatttg cgtcgtcgtg
    21061 tgcggcacaa agaacaaaga agaggttttg acccatgaca gataaacgca aagatggctc
    21121 aggcaaattg ctgtattgct ctttttgcgg caaaagccag catgaagtgc gcaagctgat
    21181 tgccggtcca tccgtgtata tctgcgacga atgtgttgat ttatgtaacg acatcattcg
    21241 cgaagagatt aaagaagttg caccgcatcg tgaacgcagt gcgctaccga cgccgcatga
    21301 aattcgtaac cacctggacg attacgttat cggccaggaa caggcgaaaa aagtgctggc
    21361 ggtcgcggta tacaaccatt acaaacgtct gcgcaacggc gataccagca atggtgtcga
    21421 gttgggcaaa agtaacattc tgctgatcgg tccgactggt tccggtaaaa cgctgctggc
    21481 tgaaacgctg gcgcgcctgc tggacgtccc gttcaccatg gccgacgcaa ccacgctgac
    21541 cgaagccggt tatgtgggcg aagacgttga aaacatcatt cagaagctgt tgcaaaagtg
    21601 cgattacgac gtacagaaag cgcagcgcgg gattgtctac atcgatgaaa tcgacaagat
    21661 ttctcgtaag tcagacaacc cgtctattac ccgtgacgtt tccggtgaag gcgtacagca
    21721 ggcactgttg aaactgatcg aaggtacggt agctgctgtt ccaccgcaag gtgggcgtaa
    21781 acatccgcag caggaattct tgcaggttga tacctctaag atcctgttta tttgtggcgg
    21841 tgcgtttgcc ggtctggata aagtgatttc ccatcgtgta gaaaccggct ccggcattgg
    21901 ttttggcgcg acggtaaaag cgaaatccga caaagcaagc gaaggcgagc tgctggcgca
    21961 ggttgaaccg gaagatctga tcaagtttgg tcttatccct gagtttattg gtcgtctgcc
    22021 ggttgtcgca acgttgaatg aactgagcga agaagctctg attcagatcc tcaaagagcc
    22081 gaaaaacgcc ctgaccaagc agtatcaggc gctgtttaat ctggaaggcg tggatctgga
    22141 attccgtgac gaggcgctgg atgctatcgc taagaaagcg atggcgcgta aaaccggtgc
    22201 ccgtggcctg cgttccatcg tagaagccgc actgctcgat accatgtacg atctgccgtc
    22261 catggaagat gtcgaaaaag tggttatcga cgagtcggta attgatggtc aaagcaaacc
    22321 gttgctgatt tatggcaagc cggaagcgca acaggcatct ggtgaataat taaccattcc
    22381 catacaatta gttaaccaaa aaggggggat tttatctccc ctttaatttt tcctctattc
    22441 tcggcgttga atgtggggga aacatcccca tatactgacg tacatgttaa tagatggcgt
    22501 gaagcacagt cgtgtcatct gattacctgg cggaaattaa actaagagag agctctatga
    22561 atcctgagcg ttctgaacgc attgaaatcc ccgtattgcc gctgcgcgat gtggtggttt
    22621 atccgcacat ggtcatcccc ttatttgtcg ggcgggaaaa atctatccgt tgtctggaag
    22681 cggcgatgga ccatgataaa aaaattatgc tggtcgcgca gaaagaagct tcaacggatg
    22741 agccgggtgt aaacgatctt ttcaccgtcg ggaccgtggc ctctatattg cagatgctga
    22801 aactgcctga cggcaccgtc aaagtgctgg tcgaggggtt acagcgcgcg cgtatttctg
    22861 cgctctctga caatggcgaa cacttttctg cgaaggcgga gtatctggag tcgccgacca
    22921 ttgatgagcg agaacaggaa gtgctggtgc gtactgcaat cagccagttc gaaggctaca
    22981 tcaagctgaa caaaaaaatc ccaccagaag tgctgacgtc gctgaatagc atcgacgatc
    23041 cggcgcgtct ggcggatacc attgctgcac atatgccgct gaaactggct gacaaacagt
    23101 ccgttctgga gatgtccgac gttaacgaac gtctggaata tctgatggca atgatggaat
    23161 cggaaatcga tctgctgcag gttgagaaac gcattcgcaa ccgcgttaaa aagcagatgg
    23221 agaaatccca gcgtgagtac tatctgaacg agcaaatgaa agctattcag aaagaactcg
    23281 gtgaaatgga cgacgcgccg gacgaaaacg aagccctgaa gcgcaaaatc gacgcggcga
    23341 agatgccgaa agaggcaaaa gagaaagcgg aagcagagtt gcagaagctg aaaatgatgt
    23401 ctccgatgtc ggcagaagcg accgtagtgc gtggttatat cgactggatg gtacaggtac
    23461 cgtggaatgc gcgcagcaag gtcaaaaaag acctgcgtca ggcgcaggaa atccttgata
    23521 ccgaccatta tggtctggag cgcgtgaaag atcgcatcct tgagtacctc gcggttcaaa
    23581 gccgtgtcaa caaaatcaag ggaccgatcc tctgcctggt agggccgccg ggggtaggta
    23641 aaacctccct gggtcagtcc atcgccaaag ccaccgggcg taaatatgtc cgtatggcgc
    23701 tgggcggcgt gcgtgatgaa gcggaaatcc gtggtcaccg ccgtacttac atcggttcta
    23761 tgccgggtaa actgatccag aaaatggcga aagtgggcgt gaaaaacccg ctgtttctgc
    23821 tcgatgagat cgacaaaatg tcttctgaca tgcgaggcga tccggcctct gcactgcttg
    23881 aagtgctgga tccagagcag aacgtagcgt tcagcgacca ctacctggaa gtggattacg
    23941 atctcagcga cgtgatgttt gtcgcgacgt cgaactccat gaacattccg gcaccgctgc
    24001 tggatcgtat ggaagtgatt cgcctctccg gttataccga agatgaaaaa ctgaacatcg
    24061 ccaaacgtca cctgctgccg aagcagattg aacgtaatgc actgaaaaaa ggtgagctga
    24121 ccgtcgacga tagcgccatt atcggcatta ttcgttacta cacccgtgag gcgggcgtgc
    24181 gtggtctgga gcgtgaaatc tccaagctgt gccgtaaagc ggttaagcag ttactgctcg
    24241 ataagtcatt aaaacatatc gaaattaacg gcgacaacct gcatgactac cttggtgttc
    24301 agcgtttcga ctatggtcgc gctgataacg aaaaccgtgt cggtcaggta accggtctgg
    24361 cgtggactga agtgggcggt gacttgctga ccattgaaac cgcgtgcgtt ccggggaaag
    24421 gcaaactgac ctataccggt tcgctcggcg aagtgatgca ggagtccatt caggcggcgt
    24481 taacggtggt tcgtgcgcgt gcggaaaaac tggggatcaa ccctgatttt tacgaaaaac
    24541 gcgacatcca cgtccacgta ccggaaggtg cgacgccgaa agatggtccg agtgctggta
    24601 ttgctatgtg caccgcgctg gtttcttgcc tgaccggtaa cccggttcgt gccgatgtgg
    24661 caatgaccgg tgagatcact ctgcgtggtc aggtactgcc gatcggtggt ttgaaagaaa
    24721 aactactggc agcgcatcgc ggcgggatta aaacagtgtt aattccgttc gaaaataaac
    24781 gcgatctgga agagattcct gacaacgtaa ttgccgatct ggacattcat cctgtgaagc
    24841 gcattgagga agttctgact ctggcgctgc aaaatgaacc gtctggcatg caggttgtga
    24901 ctgcaaaata gtgacctcgc gcaaaatgca ctaataaaaa cagggctggc aggctaattc
    24961 gggcttgcca gccttttttt gtctcgctaa gttagatggc ggatcgggct tgcccttatt
    25021 aaggggtgtt gtaaggggat ggctggcctg atataactgc tgcgcgttcg taccttgaag
    25081 gattcaagtg cgatataaat tataaagagg aagagaagag tgaataaatc tcaattgatc
    25141 gacaagattg ctgcaggggc tgatatctct aaagctgcgg ctggccgtgc gttagatgct
    25201 attattgctt ccgtaactga atctctgaaa gaaggggatg atgtagcact ggtaggtttt
    25261 ggtacttttg ccgttaaaga gcgtgctgcc cgtactggcc gcaacccgca gaccggtaaa
    25321 gagatcacca tcgctgccgc taaagtaccg agcttccgtg caggtaaagc actgaaagac
    25381 gcggtaaact aagcgttgtc cccagtgggg atgtgacgaa gttcaagggc gcatctactg
    25441 atgtgccttt tttatttgta ttcggtgact ttctgcgtct tgtgggctga caattgcccc
    25501 cgtttcttgt cacaataggc ctttgcgcgc atcgatacgt tgcgtgaggt acacagtcat
    25561 ctacagcgga gtgttgttac accatgatgg acagcttacg cacggctgca aacagtctcg
    25621 tgctcaagat tattttcggt atcattatcg tgtcgttcat attgaccggc gtgagtggtt
    25681 acctgattgg cggaggcaat aactacgccg caaaagtgaa tgaccaggaa atcagccgtg
    25741 ggcaattcga gaatgccttc aacagcgagc gtaatcgcat gcagcaacag ctgggcgatc
    25801 aatactccga gctggcagcg aacgaaggct atatgaaaac cctgcgtcaa caggtgctga
    25861 atcgtctgat cgacgaggcg ctgctggatc agtacgcacg tgagctgaaa ctgggtatca
    25921 gcgatgagca ggttaaacag gcgattttcg cgaccccagc cttccaggtt gatggcaaat
    25981 ttgataacag ccgctataac ggtatcctca accagatggg gatgaccgcc gatcagtacg
    26041 cccaggcgct gcgtaaccag ctcactaccc aacagctgat taacggcgtt gctggtaccg
    26101 attttatgct gaaaggtgaa accgacgagc tggcggcact ggtcgctcaa caacgcgtgg
    26161 tgcgtgaagc gactatcgat gttaacgcgc tggcggcgaa gcagcctgtg accgaacagg
    26221 aaattgccag ctactacgaa caaaacaaaa acaatttcat gacgccggaa caattccgcg
    26281 tgagttacat caagctggat gccgcaacga tgcagcaacc ggttagcgat gcggatctcc
    26341 agagctacta cgatcagcat caggatcaat tcacccagcc gcagcgtacc cgttacagca
    26401 tcatccagac caaaactgaa gatgaagcga aagcggtact tgatgagctg aataaaggcg
    26461 gtgattttgc tgcattagcc aaagaaaaat ctgccgatat tatctctgct cgtaacggcg
    26521 gcgatatggg ttggttagaa gatgccacta tcccggatga actgaaaaat gctggtctga
    26581 aagaaaaagg ccaactgtct ggtgtcatca aatcttcggt cggtttcctg attgtacgtc
    26641 tggacgacat tcagccagcg aaagtgaaat cgttagacga agtacgtgac gatgtcgcgg
    26701 cgaaagtgaa acacgaaaaa gccctcgatg cgtactacgc gctgcagcag aaagtgagcg
    26761 atgcggcaag caacgacacc gagtctctgg ccggtgcaga gcaagctgcc ggcgttaaag
    26821 ccactcagac gggttggttc agcaaagaga acctgccgga agagttgaac ttcaagccgg
    26881 ttgctgacgc tatctttaac ggcggtctgg taggtgaaaa cggcgcgccg ggcatcaact
    26941 ctgacatcat caccgtagac ggcgaccgcg cattcgtgct gcgcatcagc gagcacaaac
    27001 cggaagcggt gaaaccgttg gcagatgttc aggaacaagt taaggcactg gttcagcaca
    27061 acaaagctga acaacaggcg aaagtggatg ctgagaaact gctggttgat ttgaaagccg
    27121 gcaaaggtgc ggaagctatg caggctgccg gtctgaaatt tggcgagccg aaaaccttaa
    27181 gccgttccgg tcgtgacccg attagccagg cggcgtttgc actgccactg ccagcgaaag
    27241 acaaaccgag ctacggtatg gcgaccgata tgcaaggtaa tgtggttctg ctggcgctgg
    27301 atgaagtgaa acaaggttca atgccggaag atcagaaaaa agcgatggtg cagggtatca
    27361 cccagaacaa cgcacaaatc gtatttgaag ctctgatgag taacctgcgt aaagaggcga
    27421 aaatcaaaat tggcgatgcg ctggaacagc aataatcctg aagccgcctc gcaaaaaaat
    27481 gctttgcagc tgtaacaaga aaaggtcgct ttcgcggcct tttccatttc tgaacattgc
    27541 catttgttta ctgttttcac tcccgttaag gtgattccac tgttaacaaa caaggagaaa
    27601 acagtatgaa acacggaatt aaagcactgc tcattaccct gtccctggcc tgtgccggaa
    27661 tgtctcatag cgcgctggcg gcagcttctg tggcgaaacc gacggcggta gaaaccaaag
    27721 cggaagctcc tgcagcacaa agtaaagcag cagtaccggc gaaagccagt gacgaagaag
    27781 gcacccgggt cagcattaat aatgccagcg cggaagagct agcccgcgcg atgaatggcg
    27841 ttggcctgaa gaaagcgcag gcgattgtca gttatcgtga agagtatggt ccgtttaaaa
    27901 cggtggagga tctaaagcag gtgccgggga tgggcaattc gctggtggaa cgtaatctgg
    27961 cggtattaac cctctaatta atttgcatag tggcaatttt ttgccagact gaagaggtca
    28021 taccagttat gacctctgta cttataacag tttttataaa aacaacgtaa ggttattgcg
    28081 ctatgcaaac acaaatcaaa gttcgtggat atcatctcga cgtttaccag cacgtcaaca
    28141 acgcccgcta ccttgaattt ctcgaagaag cccgctggga tgggttggaa aatagtgaca
    28201 gttttcactg gatgacggcc cataacatcg ctttcgtggt ggtcaatatc aatattaact
    28261 atcgtcgccc agcggtatta agcgacttgt taaccattac gagccagtta cagcaattaa
    28321 acggtaaaag cggcatctta agccaggtca ttaccctgga gccggaaggg caggtagtag
    28381 cggatgcgct tattaccttt gtttgtattg atcttaaaac gcagaaagca ttagctctgg
    28441 aaggggaatt gcgcgaaaag ctggagcaga tggttaagta aacgttttgt ggtgccggat
    28501 gctcaagccg catccggcga cacccggaat aattacttca acccggtttt ctgcttcatc
    28561 gctgccatca ccgtcggttt atcggccaga taatgattca aaccgttggc gcgtaaatta
    28621 catgccgcac aatgaccgca accgtcgcct ttaatgccgt tatagcaggt caacgtttcg
    28681 ttacggacta aatccagttt gccgtaataa tctgccagcg cccaggtttc cgctttatca
    28741 atccacatca gcggcgtttc aaaacgaata tctttcgcca tgcccaaact gacggcatgg
    28801 tttagtgctt tcacaaactc atcgcggcaa tccgggtagc cggagaaatc cgtttcgcag
    28861 acgccggtaa ttacggcttc tgcttttacc tgatacgcat atattgccgc cagcgtcagg
    28921 aacaaaatat tacgtcctgg gacaaacgta ttcgggatgc catcggcttc aggttcataa
    28981 tcaggcaccg gaatgctgtc acgcgtcagg ctgctgaccg ccagctcgtt gagcagcgtt
    29041 acatcaagaa ccttatgcgc gcgtgctccc agtttcagcg ccagttcgcg tgccacgtcg
    29101 atttctgcgc gatgtcgctg accgtaatcg aacgttacgc aatggacttc atcatattgt
    29161 tgtaatgcct gcaccagaca ggtggtggaa tcctggcctc cactgaacac aacgacagca
    29221 cgtttcatag ataatcctgc ttgaacaata aaagcgttat ggtaacgcct gcgattagcc
    29281 cggaccagct attcattgcg acggcgcggg aagccaggct tcggtaaatt caaaccagcc
    29341 gcgcggtgtc agtcgcacac cgttcacgcc gggaggggca ctaatgcgat agtgatagtt
    29401 gaatagcggc gtcagcgtcg cgtctgccat taactggcta aaaaccgttt tcagggcatt
    29461 aaatcggttt tcctcatcag gcattacttg caccgcgtcc agtgtcgatt gcaaatgggc
    29521 atatgctgga gcgtcgaaaa catgtggcca cagtggatcg cagcgcagcc attgctccag
    29581 agtatattcc ggtgcttcgc caattaatct gtcgcccatc atgaggtctg cgtgtgccag
    29641 tagggtcgtg tcgtcccagt tttttgcgtt atgaaaaata attgtgagtt cacagccttc
    29701 cgctgccagt gtcgcctgta ggcgttctgc catggtatga agttctatcg gtaggtgata
    29761 aaccagcgtc aaggttttcg gtagtttgac ttcatccggt acttgccaat gcggaatagt
    29821 ccagcctggc agtaatgcat gactggcggt gatcaggttt tctccgactt ctaacgtttg
    29881 taataaaccg gattgatgaa taatggagat tacttttcgc gcctgccaga gggagagtcg
    29941 gggacttttg cgcaacgtta aatagcaaaa acctaaactg atgccactac tgacctggct
    30001 gacccgttgc agctcctccg gtttgccgat ggtgatttgc acgggatgcc gacaactggt
    30061 tcccaaatct ttttcgaaaa gcggcggagt tatccagtac tcaaccgctt taagcagcgg
    30121 atgacgtaaa tggtaataat catggctttc caggcgcacc agctccgctg tgaattgtgt
    30181 taagcgaaaa gaacccgtgc cgatcagtgg gaattgcgga tgcgccagat ggctgcaata
    30241 gctcgccagc cggtgcgcaa gccagtaatc agggcgatgt aaaaagaagg tcagacactg
    30301 cggatgggtg acttcaatac gcttcacgct aataaataat tgatccagtg ctggcagttg
    30361 taacagcatc aataatcgct ggtgtaagtg tgaggctttt actgcatcgc cgttatgcca
    30421 gtgtagggtt gaacgaagat aaaagtccca gcgtaacccg tcagtagagg tttcccagtg
    30481 atgcgctaaa tcgccaatcg ggcgctgggt attattatcg aagcgggtca ggccggaaaa
    30541 tatctgtccg gcgagatgct gctcggcacg gccgggcaaa aagcccggtt gtagcggttc
    30601 gagcgggcga tagtagggaa tacgcaacgt gggcgtatcg ttttgccatt gtccgcccat
    30661 aaacggctgt aacagagtgc gcagctcacc tggggccagt tgcgccagct ccagcacatc
    30721 ttgctgcttt ccggtttcca gtgccttttc catcatcgca ttgcgtagcg attctggcgt
    30781 gaccagaaag cgtaattgtc cgtgctttcc gcgtcctgac tgcgcctgcc actccagcca
    30841 tcccgcctct tgtgcctgac gcaacagcgt acgaacatgg cgttcgctgc aaaaacagcg
    30901 ttcggccagt tcgctgacgg tgacggtttg cggctttccg gcagaaggtt gccacagacg
    30961 ttgatactgg ttaagacggt tgagcagtcg catataaacc cggaacaata ttatttaact
    31021 attcactatt acttccgtat atatcaggtg atactcaatc accattaacc gtgtcacaga
    31081 gtggagaaag aaatggctcg tctggcagca tttgatatgg atggcacttt attgatgcct
    31141 gaccatcatt taggtgagaa aaccctctct actttggcgc gactgcgtga acgcgacatt
    31201 accctcactt ttgccacggg gcgtcatgcg ctggagatgc agcatattct cggggcgcta
    31261 tcgctggatg catatttgat taccggcaac ggaacgcgcg tgcattctct ggaaggtgaa
    31321 cttttacatc gtgatgattt acctgcggat gtcgcggagc tggtgctgta tcagcaatgg
    31381 gatacccgag ccagcatgca tatcttcaat gacgatggtt ggtttaccgg gaaagagatc
    31441 cctgcgttgt tgcaggcatt tgtctatagc ggttttcgtt accagataat cgatgtcaaa
    31501 aaaatgccac tcggcagcgt caccaagatc tgcttctgtg gcgatcacga cgatcttaca
    31561 cgcttgcaga tccagctata cgaagcatta ggcgagcgtg cacatttgtg tttttccgcc
    31621 acggattgcc tcgaagtgct gccggtgggc tgcaataaag gcgctgcact gacggtgctg
    31681 acccaacatt taggtttatc gttgcgcgat tgcatggcct ttggtgatgc gatgaacgat
    31741 cgcgaaatgt taggcagcgt cggtagcgga tttattatgg gcaatgcgat gccgcaactg
    31801 cgcgcggagc tcccgcattt accggtgatt ggacattgcc gaaatcaggc tgtctctcac
    31861 tatttgacgc actggctgga ctatccacat ctaccttatt cccccgaata acgagatccc
    31921 ttccagcacc gggcaattgc ccggtttttt ttgcgttgaa tttgtcattt tgtgccgtgg
    31981 tgtttaaacc gcacagaata aattgtcgcg atttcacctt taaaatagaa ttaaaagaga
    32041 aaaaaattct ctgtggaagg gctatgttag ataaaattga ccgtaagctg ctggccttac
    32101 tgcagcagga ttgcaccctc tctttgcagg cactggctga agccgttaat ctgacaacca
    32161 ccccttgctg gaagcgcctg aaacggctgg aggacgacgg tatccttatc ggcaaagtcg
    32221 ccctgctgga tccggaaaaa ataggcctcg gcctgaccgc ctttgtgctg ataaaaacgc
    32281 aacatcacag cagcgaatgg tattgccgct ttgtcacggt ggttaccgaa atgccagaag
    32341 tgctggggtt ctggcgcatg gctggtgaat acgattatct gatgcgcgtc caggttgccg
    32401 acatgaaacg ctacgacgag ttttataagc gtctggtaaa cagcgtgccg gggctgtcgg
    32461 acgtcacttc cagcttcgcg atggaacaga ttaaatacac cacttcttta cccatcgaat
    32521 aaatatccag aatcaggtca ggacacaacg cgtgcgatta tttgctcaat taagctggta
    32581 tttccgtcgg gaatggcgtc gctatctcgg ggctgtcgcg ttgcttgtca ttatcgcgat
    32641 gctgcaactg gttccaccaa aagtggttgg tattgttgtc gatggcgtga cagaacaaca
    32701 ctttactacc gggcagatcc tgatgtggat cgccaccatg gtgctgattg ccgttgtggt
    32761 ttatctcctg cgttacgtct ggcgcgtatt gctgtttggt gcgtcttatc aactggctgt
    32821 tgaactgcgt gaagattatt accgtcagct aagccggcag catcctgagt tttacctgcg
    32881 tcatcgcacg ggtgacctca tggctcgtgc gactaatgac gtcgatcgcg tcgtgtttgc
    32941 cgccggagaa ggggtgctga cgctggtgga ttcactggta atgggctgcg ctgtgttgat
    33001 tatgatgtct acgcaaatta gctggcagtt gaccttattt gccctgttac cgatgccagt
    33061 gatggcgatc atgattaagc gcaacggcga tgctttgcat gaacgcttta agctggcaca
    33121 ggcggcgttt tccagtctta atgaccgcac ccaggaaagc ctcaccagta tccgcatgat
    33181 caaagccttt ggtctggaag atcgccagtc ggcgttattt gccgcggatg ccgaagatac
    33241 cggcaaaaaa aacatgcggg tggcgcgtat tgatgctcgt ttcgacccga ccatctatat
    33301 cgcgattggt atggcgaact tgctggcgat tggcggcggt agttggatgg tggtgcaggg
    33361 cagtttaacg ctgggccagc tcaccagttt tatgatgtat ttaggtctga tgatttggcc
    33421 aatgctggcg ctggcatgga tgtttaacat tgtggaacgt ggtagtgctg cgtacagccg
    33481 tattcgcgcg atgctggcgg aagcgccggt ggtgaacgat ggtagcgaac ccgtgccgga
    33541 agggcgtggc gaactggatg taaatattca ccagttcacg tatccgcaga ctgaccatcc
    33601 tgcgcaggaa aacgtcaatt tcgccctgaa acccggtcag atgctgggta tctgcgggcc
    33661 gactggttcc ggcaaaagta ccctgttgtc gctcattcag cgtcatttcg acgtcagcga
    33721 gggggatatt cgctttcatg atattcctct gacgaagtta caactcgata gctggcgtag
    33781 ccgcctggcg gtagttagcc agacgccatt ccttttttct gacaccgtgg cgaataacat
    33841 cgcgctgggt tgcccgaatg ccacccagca agagattgag catgtcgcgc ggttagccag
    33901 cgtacatgac gatattttgc gtctaccgca aggttacgat acagaggtgg gcgagcgcgg
    33961 tgtgatgctt tccggcgggc aaaaacagcg tatctccatt gctcgtgcgt tattagtcaa
    34021 cgcggaaatc ctcatccttg atgatgcgct ttcggcggtg gacggacgca cagagcacca
    34081 gatcctgcat aacctgcgtc agtgggggca gggaagaacg gtaatcatca gtgcccatcg
    34141 cctttctgca ctgacggaag ccagtgaaat tattgtgatg cagcacggac atatcgccca
    34201 gcgtggcaat catgatgagc tggcgcaaca aagtggctgg tatcgcgata tgtatcgcta
    34261 tcaacaactg gaggcggcgc tcgacgacgc tccggaaatt cgcgaggagg ccgtcgatgc
    34321 gtagttttag ccaactgtgg ccgactctca agcgtctgtt agcgtacggt tcgccgtggc
    34381 gtaaaccgct ggggattgcg gtcctgatga tgtgggttgc ggcagcggca gaagtcagtg
    34441 ggccgctgct tatcagctat tttatcgaca atatggtagc gaaaaataac ctgccgttga
    34501 aagtggttgc agggctggct gcggcgtatg ttgggctaca actgtttgcc gccgggctac
    34561 attacgcgca gtcgctgctg tttaatcggg cggcagtagg cgtagtgcaa cagttgcgta
    34621 ccgacgtgat ggatgctgcg ttacgccagc cattaagcga gtttgatacc caacccgtcg
    34681 ggcaggtgat ttcccgcgtc actaatgaca ctgaagtgat ccgcgatctc tacgttaccg
    34741 tagtggcaac tgtcctgcgc agtgccgcac tggtgggcgc gatgctggtg gcgatgttca
    34801 gcctcgactg gcgaatggcg ctggtggcga taatgatttt cccggtggtg ctggtggtaa
    34861 tggtgatata ccagcgttac agcacgccga ttgtccgtcg tgtgcgcgcc tatctggcgg
    34921 atatcaacga cggctttaac gaaatcatca atggcatgag cgttatccag cagttccgcc
    34981 agcaggcgcg atttggcgaa cgcatgggtg aggccagtcg ttcacactat atggcgagga
    35041 tgcaaaccct gcgcctcgac ggttttctcc tgcgtccgct gctgagtctg ttttcatcgc
    35101 tcattctctg tggcttgttg atgctgtttg gcttctccgc cagcggcacc attgaagtgg
    35161 gcgtgttgta tgcgtttatc agctatcttg ggcgacttaa cgaaccctta atcgaactga
    35221 ccacgcagca ggcgatgctg caacaggctg ttgttgctgg tgagcgcgtg tttgagctga
    35281 tggacggacc gcgccagcaa tatggcaatg atgatcgccc gttacagagc ggcaccatcg
    35341 aagtcgataa cgtgtcattt gcttatcgcg ataacaatct ggtgctaaag aacattaatc
    35401 tctctgtgcc ttcgcgcaat tttgtggcgc tggtcgggca taccggcagt ggcaaaagca
    35461 ccctcgccag tttattgatg ggctattacc cgctaacgga aggtgagatt cgccttgatg
    35521 gtcgtccatt aagttcgcta agtcacagcg cgctgcgcca gggcgtggca atggtgctgc
    35581 aagatccggt ggtgctggcg gataccttcc tcgccaacgt gacgctgggg cgtgatatct
    35641 ccgaagaacg cgtctggcag gcgctggaaa ccgtgcaact ggcggagctg gcgcgtagca
    35701 tgagcgacgg tatttacacg ccgctgggcg agcaggggaa taatctctct gtcgggcaaa
    35761 agcaactgct ggcactggca cgcgtgttgg tggagacgcc gcaaatcctg atccttgatg
    35821 aggcaaccgc cagcattgac tccggtactg aacaggcgat tcaacatgct ctggcggcgg
    35881 tgcgtgaaca taccacgctg gtggtgattg ctcatcgctt atcgaccatt gttgatgccg
    35941 acaccattct ggtgcttcat cgtgggcaag ccgtggagca gggcactcac cagcaactgc
    36001 tggcggccca gggacgctac tggcagatgt atcaactgca acttgcgggc gaagagctgg
    36061 cagccagcgt gcgtgaagag gaatcattga gcgcctgaat agcgcaatat ttcatcgttg
    36121 gtgcaaaaat gtaacgcact gtgcactgtc atagtgcgtt ttcattttca aacttcttaa
    36181 cttcctgctc tctttctcgt ttttcatttc tggcacaccg cttgcaatac cttcttcgtg
    36241 tagcagaacc attaccgaat tctgaccgga ggggatctat gaagctggtg accgtgataa
    36301 tcaaaccatt caagctggaa gacgttcgtg aagcgttatc ttccattggt attcagggcc
    36361 tgaccgtcac cgaagtgaaa ggtttcgggc gtcagaaagg gcatgccgag ctgtaccggg
    36421 gggcggaata cagcgtcaac ttcctgccaa aagtaaaaat tgatgtggcg attgctgatg
    36481 accaactcga tgaagtgatc gatatcgtca gtaaggcggc ttacaccgga aaaattggcg
    36541 acggcaaaat cttcgtcgct gaattgcaac gcgtcattcg tattcgtacc ggcgaagccg
    36601 acgaagcggc gctgtaatct ctggcacaca gcaacaggaa cgaaaaatga agatagcgac
    36661 gataaaaact gggcttgctt cactggcgat gctgccggga ctggtaatag ctgcacctgc
    36721 ggtggccgat aaagccgaca atgcgtttat gatgatctgt actgcgctag tgctgtttat
    36781 gactattccg gggattgccc tgttttacgg tgggttgatt cgcggcaaaa acgtgctgtc
    36841 gatgctgacg caggtgacgg tgacatttgc actggtctgt attctctggg tggtttacgg
    36901 ttactcgctg gcgtttggtg agggcaacaa cttcttcggc aacattaact ggttgatgct
    36961 gaaaaacatc gaactgacgg cggtgatggg cagcatttat cagtatatcc acgtggcgtt
    37021 tcagggatcg tttgcctgca ttaccgtagg cttgatagtt ggggcgctgg cggaacgaat
    37081 ccgcttctca gctgtgttga ttttcgtggt ggtatggctg acgctctctt acattccgat
    37141 tgcgcatatg gtctggggcg gtggtttgct ggcttctcac ggtgcgctgg atttcgcggg
    37201 tggcaccgtg gtgcacatta acgccgcaat cgccggtctg gtgggcgcgt atctgatagg
    37261 caaacgcgtg ggcttcggca aagaggcgtt taaaccgcac aacctgccga tggtcttcac
    37321 cgggactgcc attctctata tcggttggtt tggctttaac gccgggtcag cgggcacggc
    37381 gaatgaaatc gcggcactgg catttgtgaa tactgtggtc gcaacggcgg cagcaattct
    37441 tggctggatc ttcggtgaat gggcgctgcg cggtaagcct tcactgctgg gagcgtgttc
    37501 tggcgcgatt gccggtctgg tcggcgtgac gccagcgtgt ggctacattg gtgttggcgg
    37561 cgcgttgatt atcggcgtgg tagctggtct ggcgggcttg tggggcgtta ccatgctcaa
    37621 acgcttgctg cgggtggatg atccctgcga tgtcttcggt gtgcacggcg tttgtggcat
    37681 tgtcggctgt atcatgaccg ggatttttgc cgccagctcg ctgggcggcg tgggcttcgc
    37741 tgaaggtgtg acgatgggcc atcagttgct ggtacagttg gaaagcattg ccattacgat
    37801 cgtctggtcc ggtgtggtgg catttattgg ctacaaactg gcggatctga cggttggcct
    37861 gcgtgtgccg gaagaacaag agcgagaagg gctggacgtc aacagtcacg gcgagaatgc
    37921 ctataacgcg taacaagcac tgcaaaaaaa tagccggacg gttttcacct ccggctattt
    37981 ttttaattgt gattacgcat caccccttcc tgaacggtcg aggcaaccag tacgccgtcc
    38041 tgggtataaa actcaccgcg cacaaagcca cgtgcgctgg acgccgaggt gctctccacg
    38101 ctatacagca gccattcatt caaattaaac gggcgatgga accacatgga atggtcaatg
    38161 gtggcaatct gaatccccgg ttcgagaaaa ccgatgccgt gcggctgtag agctaccggc
    38221 aggaagttta gatcagaagc gtaaccgagc agatactgat gaacgcgcag gtcatccggc
    38281 acgctaccat ttgcgcggat ccacacctga cgatgtggtt ctgcgacgtg acctttcagt
    38341 gggttatgaa actccacagg acggacttcc agcggacgat cgcagatgaa tttatctttc
    38401 agcactggcg gcagcaggtg cgccagcgac tgggcgattt gcgtttccga aggcaggcca
    38461 tcaggcgctg gcgcggacgg cattgttttt tgatgttcga aacccgcttc tggtgcctgg
    38521 aaagaggccg tcatataaaa aatcggtttg ccgttttgaa tagcagcaac ccggcgggcg
    38581 ctgaagctgt taccgtcacg cagcgtttcg acatcataaa taatcggctt cttactatcg
    38641 ccagggcgaa gaaagtagct gtgaaacgaa tgtaccagcc gctcttcagg gacggtctct
    38701 tttgcagcat acaaggcctg acccacgacc tggccgccaa acacctggcg taaacctaaa
    38761 tcttcactct ggccgcgaaa gagtccttcc tcaatttttt ccagatttaa caatgtcagt
    38821 aaatttttta gcgcctgact catataactc tccagtaaca aagctgccgc agcaagccaa
    38881 agtgagttga gtataacgca aatttgctac tggtccgatg ggtgcaatgg tctgaattac
    38941 gggctaatta caggcagaaa tgcgtgatgt gtgccacact tgttgacgtt actattttgt
    39001 taaccactct tccggcgagg aaagttagcc cgctggtgca ttgataataa ggagaaatga
    39061 atgaaactcg tgcacatggc cagtggttta gcggttgcga ttgcgttggc ggcttgcgca
    39121 gataaaagcg cggatattca gacgccagcc ccggctgcaa atacgtctat ttcagcaaca
    39181 caacaacctg ctatccagca accgaatgtc tccggtaccg tctggatccg tcagaaagtc
    39241 gcactgccgc ctgatgctgt gctgaccgtg acactttctg acgcgtcgtt agccgatgca
    39301 ccgtcaaaag tgctggcgca gaaagcggtg cgtactgaag gtaaacagtc accattcagc
    39361 tttgttctgc catttaaccc ggcaaatgtt cagccgaacg cgcgtattct gttgagtgcg
    39421 gcgattaccg tgaatgacaa actggtattt atcaccgata ccgttcagcc ggtgatcaac
    39481 cagggcggaa ctaaagccga cctgacattg gtgccggtac agcaaaccgc cgtgccggtt
    39541 caggccagcg gtggcgcaac gactaccgta ccttcgactt caccaactca ggtgaatccg
    39601 tcttcggcag ttccagctcc tacgcaatat taagcgacgt taaccctctc cgcccggaga
    39661 gggttagtag ttccagcgat aacgctgcaa gtcgatttgc ccgcttcccg ataccatcac
    39721 accttctgcc agtaatgcct gtcgctgacg ctgtaaatcc ggtccggtta gcgaaattgt
    39781 gccgtggcga ttaaccaccc ggtgccaggg taaggtgctg ccttcaggga gacgctttaa
    39841 cacaccgccc acctggcgcg cggcgcgggg cgatcccgcc agtttcgcca catcaccgta
    39901 agtggttaca tagccttcgg gaatagcggc gacgatttgc cagacgcgtt ggggaaatga
    39961 atcttctttt tccatctttt cttcctgagt taatttttca gcataatctg gaaaaacgcc
    40021 cgagtgaagc cgcattgcgc aagaaaccag catctggcac gcaatgggtt gcaattagcc
    40081 ggggcagcag tgataatgcg cctgcgcgtt ggttctcaac gctctcaatg ggggctctgt
    40141 tggttctccc gcaacgctac tctgtttacc aggtcaggtc cggaaggaag cagccaaggc
    40201 agatgacgcg tgtgccggga tgtagctggc agggccccca cccatttcgg cctctcaccg
    40261 tttcgtcaaa aaatcccaac atggctaaac tttaaccaca actgacgtcg caagaattgt
    40321 ctggctgcgc agtacgcttc ggaggtatgt ctgatgaagt atgttgatgg ttttgtggtt
    40381 gccgttcctg ccgataaaaa ggatgcctat cgggaaatgg ccgctaaggc tgcgccattg
    40441 tttaaagagt ttggcgcgct tcgtattgtc gaatgctggg ccagcgatgt accggatggc
    40501 aaagtgaccg attttcgtat ggcggtgaaa gcggaagaga atgaagaggt tgtctttagc
    40561 tggattgaat acccttcaaa agaggtccgc gacgcagcta atcaaaagat gatgtcggac
    40621 ccacggatga aagagttcgg cgagtccatg ccgtttgacg gcaagcgaat gatctatggc
    40681 ggattcgagt caatcatcga cgaataggta gcgtgacggg ctgtgcggcg tgccgtcagc
    40741 ccaaaaattc acaaatgttg ctcggcccag cgtaagaaat cttctttcgg taatgccttg
    40801 ctgtagagcc agccctgacc gtagtgcacg ccatgctggc gtaaccactc ttcttgttta
    40861 ctggtttcga ttccctccgc taccattttc agtttcagtg tttttgccat ttcgatgata
    40921 tgcggcgtga catttttata ttccagcgca tcaacgaaag atttatcgat cttcagaatg
    40981 tcgacatcca gatcctgtaa ataacttaaa cttgaatacc ccgtaccaaa atcatcaaga
    41041 taaatttcat ggcccgcctc ccggtagcga gaaattatcg gggcgctggt tttcggatcg
    41101 gcaaactcgc gttcagtgag ttcaagcgcg atctgtctgg gattaacctg atagtgattg
    41161 atcatttcac gcagcaattg cgggattttt tccgaggtga gcacggtgga ttcaagattg
    41221 atcgaaatat gctgctgtgg atgctgacgc agccagtcgc ccatatcttc aaagacgctt
    41281 cttataatca gtagcgtcaa tggctcagaa aggcccgttt gctgtgccag cggaataaaa
    41341 ctatctggtg acaaccaact accgtctgtc tgcggccagc gcgccagtgc ctcagcaccg
    41401 acaattttgc cattggctaa ggagacaatc ggctgatagt gcacgcaaat atcacgattt
    41461 tcgatagcat cctgcagccg atgatgcggt gactggatac ggcgcaaaat acgcagcaca
    41521 aacatcgctg ccagcaggcc aatcaccaac ccgagcggta accaaataaa gacttgccga
    41581 tgccagcctt tctgcaacat tttcgttgaa gcccaggtga taattgaaat attcatctcc
    41641 gggaagggca ggatatcgta aatgattcca ttattttcga tatgctcacc gggtgttttt
    41701 tgtagcctgg taataattcc ctgagcaatt tcatcgctgc tggttatgac aacgttatgg
    41761 gcattgccaa taatggcggc atcaatttgc catgagctat agggaatgac atcaataaag
    41821 gaagcggggt cgatcatgac gacataatgt gccgttccca tggcgaccat gtaacggata
    41881 atgcctaaat cgttatgcga tgttaaccag acacgataac catctttcga aattttacct
    41941 ggctctggga aggtatcggg cgggctttca tgctccagag acgaacactg gggaacgttg
    42001 ttatcgatat aaaccacttc ctgaatatag cgataactgt aagatacccg acgcatttcc
    42061 atgagatggg cttcgctaca ggcagcgcct tgccatcttt ccagctcctg cagcgcatct
    42121 ttcccttgtg tcgccacctt attggcccga atagcgacgc gggaggaata ggtatccagc
    42181 tcttcaataa acgatgtttc tacctgctga tgggccagcc agatgcttaa gccgacaggc
    42241 agcaatacta aaagaatcag tactcccgaa ataaggccga ccagatgtcg tgttctcacc
    42301 agcatatcct tattaacaca gtgtagggaa agtatatctg attgtggaaa aaggctggca
    42361 ggagataagg aggtagagct ggtatttcat gaagtgatgg gtggaaacga tcgttgtcat
    42421 ttgaccaggc gcagagcttt acgcctggta tgtaatccta acttaagatg ttgattcggc
    42481 gcgagcgagg gtaaaaatat cgtaaaaaga caattcacct ttacggacga tcattttttg
    42541 cagttgttcc ttatgaacat ccgcgacacg cggagagttc aagatggctt caaccagcgc
    42601 ggcaggtaca aagcgcgtgt tgtaccattc gccgttatag caaacgcgga aatccaacac
    42661 gtcgatatct tcatagcggt agcgtgggta gacatcgaaa aagaagaaac cattaagcag
    42721 gataaataat acaaccagta gccagacaga gcctgaaagc gtttcggact gcaacatcac
    42781 cgccagggtg gcaaaaaaag caacgtacat accgataaac agccccggat gtttacggat
    42841 aaaactgata ctaaagcggg gtttgttgtc gcgtttttcg cgggtattca gagactcaat
    42901 cgtttcggtc agcaggcgtt gtatttcggt catcgtttgc cttcgtggta tctgcaactt
    42961 tccagggaac accctatttt aacgtgggaa tagtagaggg aaagtaacag atttaccaca
    43021 attttgcata acagttgcga aaaaccgatt acaatttttt aattattctg gctggattac
    43081 cgcccacgac aacgttgtcc gggacatctt ttgtgacaac tgcacctgag gctaccacga
    43141 cgttatcacc aatggtcaca ccagggttaa tgaccgcgcg tccgccaatc cagacgttat
    43201 taccgatggt gacgggtttc cccagttcag caccgctatt acgtgctaca gggtcgatgg
    43261 gatgtgttgc cgtgtagata tgaacgcctg gtgccaacat acagttatca ccgatgcgaa
    43321 tagggcagac atcaagcatc acgcaatcga agttggcgaa aaaattatta ccgagaaaaa
    43381 tgttatagcc atagtcacag cgaaacgttg gctcaatata agcctctgtc acctgaccga
    43441 atagatcagc gagaatttgc tggcgtaatg tgtgctcttc cgccagggaa tgattgtatc
    43501 ggtgaataag ctgacgagcg cgcaggcgat cgcgagataa cgtctcatct gccgagcgat
    43561 acaactcacc agcaatcatc ttttcttttt ctgtgctcat tactgacctc cggttgctga
    43621 aaatgcggca acacagtaag acgaaaagtc agtgaagaga acgttcccat tacatctgtg
    43681 tgataaagat cacatagggt atgtcttcat ggcgaaaaag tataaaattc ttaataaaca
    43741 gccggttata gctccgaaag cgaattattt attagcgaat aaatttccat actgaggaag
    43801 ggatcttgtc gtacagttta ttcatggtca attcggcgag gcggtgatct gcggctgagt
    43861 aaaataccgc cagttcatta tctgataatt cgtatttatt tttctcgata acacgctcca
    43921 gcgtgtcaat tgtctggcaa cgacgtaaac gcattaaata atcggttttc gttaaaggtt
    43981 tttcggacat acttctaccc atggttgtaa taattctaac aagataaact cgcaggattc
    44041 tctttcgtcg cattgacaaa acaacggaat agtcgattac ctgacttccg ccatttctga
    44101 agatcctgca tattaatacc ataactgctg aacaacataa aggtgtcatc cagatattcg
    44161 tcgatctgct caatgagctt attgtcttca ttatacttaa ttttgtaatt aagtgcgaag
    44221 gtcgcaatat gctcaatcag ttcattcaac tggaggttga tcgccgaggt tgggtcgttt
    44281 acccagccat gattgctttc ttcaaggttt gcaaggcagt catgatacag ggtttcacag
    44341 agaaacttaa gctgtgcgat atcatgtctt ttgggtgagt attcatccat aacgcgtccc
    44401 cttcttagcg gttgaactaa cggacacctt tcgggatgga aaaaacttac tgacctggac
    44461 ttgccctctc gtttgttagc ttaactataa gccactcttt gcaggttttc atcgcattat
    44521 tacgaaaaat tacaattagg aaggaaacgg gacaggactc actttttcat actagcacta
    44581 acccacagca ggtaaaagca gtttgctcga actattcata acatttcagg attatcgcca
    44641 gatgaaattg gtgacactaa taccaggatt gctctgaata tgacgtaata accgaggaat
    44701 gaataaagaa ttaccgcaaa taattaagaa tagcctctaa atgattatgg attattaaat
    44761 ctattagcgc tacttaatat aaattaacta acaatcagta ttttttatga attttatccg
    44821 tggttaatac tggttttcgt atgagatcct gagttggtgg ttcaattact ccttaatgtt
    44881 cgtaggttat gcataaaaaa ggccgcttac gcggccttag tgattacacg atgtatcaat
    44941 gatgatcgac agtatggctg tgctcgatat cttcattctt gcggctaaag cggcggcgaa
    45001 ccaccacaaa gaataccgga acgaagaaga tagccagtac cgttgcggtc accatcccgc
    45061 ccattacacc ggtacctact gcgttctgcg cgccggaacc agcaccagta ctgataacca
    45121 gcggcataac gccgaggata aacgccagcg aggtcatcag gatcggacgt aaacgcatcc
    45181 gcactgcatc aagcgtcgct tcaatcagac ctttaccttc tttatccatc aagtctttgg
    45241 cgaattcgac gataagtatc gcgttcttcg ccgacaaccc aatggttgtg agcaggccta
    45301 cctggaagta aacgtcattg gtcaggccac ggaaggtggc agccagcaac gcaccgataa
    45361 cccccagcgg aacgaccagc ataacggaga acggaatcga ccagctctcg tacagcgccg
    45421 ccagacacag gaatacgaca atcaacgaaa tcgcgtacag tgaaggtgcc tggttgccgg
    45481 agaggcgttc ctgataggac atccccgtcc agtcatagcc aacaccggta ggcagtttgc
    45541 tcgccagttg ttccatcagc tccattgctt caccggtact tttccccggt gccgcctggc
    45601 ctaagatttc catagatggc aggccgttgt aacgttccag acgcggcgaa ccgtactccc
    45661 aacgagaaga ggagaacgcc gagaacggca ccatctgacc atcagcagca cgaacatacc
    45721 agtcgccgat atcatccggc agcatacggt atttcgcttc tgacatgacg taaactttct
    45781 tcacacgacc gcggtcgata aagtcgttca catagctgcc gccccatgca gcgcccagag
    45841 tggtgttaat gtcgttgata gaaacaccca gcgcctgcgc tttttcctgg tcgatatcaa
    45901 tcttaaactg cggggtatct tccagaccgt ttggacgtac gctggtcaac atatcagggt
    45961 gcttcgctgc ttctgcaagc aactggttac gcgcctgagt cagtttttcg tgaccaaggc
    46021 cagcctggtc aatcagctca aagtcaaagc cggttgcggt acccagttcc acgatcgcgg
    46081 gcaggttaaa ggcgaaaacc atcgcatctt tgatttgcga gaaagcgcgt gttgcacgca
    46141 tggtaatcgc ttcaactttg ttttcttcgc ccggacgatc ggcccagtcc ttcaaggaaa
    46201 cgaacgcaat accggtattc tgaccacgtc ccgcaaagcc gaagccgtta acggcgaaca
    46261 ccgactcaac gttgttcttt tctttggtca gatagtaatg cgttacctca ttgagcactt
    46321 tctgtgtacg ttcctgcgtt gcacctgctg gcagctgaac catggtcata aatacgccct
    46381 ggtcctcatc tggcaagaag gagcttggca gacgcacgaa cagataggcc atgccgacca
    46441 cgatgatcag atacagcacc aggtaacgcc ctgtactgcg cagaataccg cctacgctgt
    46501 cggtgtagtg gtgcgtgctc ttctcgaaca tgcggttaaa ccagccgaag aagccttttt
    46561 taccttcccc gtgatcgcct ttggcaatcg gtttcagcat ggtggcacaa agagccggag
    46621 tcaggatcaa cgctaccagt accgacagcg ccattgctga aacaatggta atagagaact
    46681 gacgatagat agcaccagta gaaccgccaa agaaggccat cggtacgaat accgccgaca
    46741 gtaccatcgc gataccgacc agagcgccct gaatctgccc catcgactta cgggtggctt
    46801 cttttggcgg caaaccttct tccgccatca cacgctcaac gttttctacc acaacgatag
    46861 cgtcatccac caacaggccg atggcgagca ccatcccgaa cattgttagc gtgtttatcg
    46921 agaagccaaa ggcggcaagg acggcaaagg tcccgagcaa taccaccggt acggcaatgg
    46981 tcggaatcaa cgtcgcgcgg aagttctgca ggaacagata cataaccagg aacacgagga
    47041 tgatcgcttc gaccagcgtt ttaaccactt cgtgaataga gattttcacg aacggcgtgg
    47101 tgtcgtacgg gtaaacaatt ttcagacccg acgggaagaa cggttccatc ttcgccagtt
    47161 cagcacggat tgccgcagcg gtatccagcg cgtttgcacc ggtcgccagc ttgatcccca
    47221 gaccggaagc cggttggccg ttaaactctg cgatgatgtc gtagttctca ccacccagct
    47281 caatcttcgc cacatcacgc agcagcacgc gggaaccatc ctgattcact ttcagcagga
    47341 ttttgccgaa ctcttcagta gaggtcagac gcgtctgagc aataatagag gcgttaagct
    47401 gttggccttt caccggcggc gtaccaccga gctgacccgc cgcaacctgg gcgttctgcg
    47461 ctttgatggc ggtaatgaca tcaaccggcg ttagctggaa tttgttcagc tcattcgggt
    47521 tcatccagat acgcatcgcg tactgtgaac cgaacaactg aacgtcaccc acgcccgacg
    47581 tacggctgat ggcatctttc atattcgccg ccacatagtc ggagatatcc tcctgcgtca
    47641 tggtgccatc ggtgttgata acgccgacaa ccatcaggaa gctgctggat gatttctcaa
    47701 cgctcacccc ttgctgctga acttcttgcg gcagcaacgg catcgccagc tgcagtttgt
    47761 tctgcacctg aacctgcgcg atatccgcat cagtaccaga ctcaaaggtc agggtgatct
    47821 gcacggtacc cgtggagtca ctgttagagg acatgtacat caggttatcg ataccgttca
    47881 tattctgttc gataacctgt gtcaccgtgt cctgcactgt tttcgcatca gcgccggggt
    47941 aggaggcgga gatcgttact gccggcggtg caatcgtagg atattgcgcc accggcagtt
    48001 tgaggatcgc cagcccccct gccaacatga tgataatggc gatcacccac gcaaaaatcg
    48061 ggcgatcgat aaagaaatta ggcatgtctt aacggctcct gtttaagtta agacttggac
    48121 tgttcaggct gagcaccgct tgcggcttgc tggttattat cagcggtaac ttcttgtgct
    48181 tttacctgga caccaggacg cactttctgc agcccactta ttactacgcg atcgcctgct
    48241 ttcagacctt ctgtcaccag ccacttatcg ccaatagcct ggcttgcaac gatcggacgg
    48301 gtttccactt tgtcatccgc gccaactacc agtacggtgg catcgccacg cggcgtacgg
    48361 gttacgccct gttgcgggac taaaatagcg tttggattaa gcccttcttc cagacgtgcg
    48421 cgcacgaaca tacccggcag cagagtgtga tccgggttcg ggaagatagc gcgtagggtg
    48481 atagacccag tggtctgatc aacggtaacg tcagagaatt ccagcgtacc gtcctgcggg
    48541 aacttaatgc cgtcactggt gatcagcgac actttggctt tgccgttctc ttgtttcagc
    48601 gtgccattcg ccagttcctg tttcaggcgc aggaagtcgt tgctggactg ggtcacatca
    48661 acgtagatcg gatcaagttg ctgcacggtt gccagcgcag tcgcctgacc gttctgtacc
    48721 aatgcgcctt ccgtcacgtt cgacttacca atgcgaccgc taatcggaga ggtgactttg
    48781 gtgtaagcca gattgatccg cgcagtttca acagcagctt tcgccgcagt taccgcagca
    48841 ttcgcctgtt gcgcatcagc cagagcctga tcgtactctt gcttactgat gtactgagta
    48901 ccgagcagtt tctgataacg attcaccgtc aattgcgcga tattggctgc agcctgggct
    48961 ttcgccagat cacctttcgc actgtcgtat gtcgcctgat aggtcgcagg atcaatctga
    49021 tagagagaga cacctgcttc gatgtcgcta ccttctttga aattacgctt caggataatc
    49081 ccgctaactt gaggacgaac ttctgcgatc cggtaggcac tggtgcgacc cggaagctcg
    49141 gttgtgatct gcagaggttc agttttgact gttactacgc caacggcggg catctgctgg
    49201 ccaccttgtt gggcctgttt gtcgtcacat cctgttaggg ctaagctgcc tgagagcatc
    49261 agaacgaccg ccagaggcgt aaaccctctg tttttgttca tatgtaaacc tcgagtgtcc
    49321 gatttcaaat tggtcaatgg tcaaaagtta ataaacccat tgctgcgttt atattatcgt
    49381 cgtgctatgg tacatacatt cacaaatgta tgtaaatcta acgcctgtaa attcacgaac
    49441 atatggcacg aaaaaccaaa caagaagcgc aagaaacgcg ccaacacatc ctcgatgtgg
    49501 ctctacgtct tttctcacag cagggggtat catccacctc gctgggcgag attgcaaaag
    49561 cagctggcgt tacgcgcggt gcaatctact ggcattttaa agacaaatcg gatttgttca
    49621 gtgagatctg ggaactgtca gaatccaata ttggtgaact agagcttgag tatcaggcaa
    49681 aattccctgg cgatccactc tcagtattaa gagagatatt aattcatgtt cttgaatcca
    49741 cggtgacaga agaacggcgt cgattattga tggagattat attccacaaa tgcgaatttg
    49801 tcggagaaat ggctgttgtg caacaggcac aacgtaatct ctgtctggaa agttatgacc
    49861 gtatagaaca aacgttaaaa cattgtattg aagcgaaaat gttgcctgcg gatttaatga
    49921 cgcgtcgcgc agcaattatt atgcgcggct atatttccgg cctgatggaa aactggctct
    49981 ttgccccgca atcttttgat cttaaaaaag aagcccgcga ttacgtcgcc atcttactgg
    50041 agatgtatct cctgtgcccc acgcttcgta accctgccac taacgaataa ccctgaatct
    50101 gactccagga tttttcctgg acattttcgt cgttgctatt ctggttcact gcgtcgtgat
    50161 attcttgcgg tttgactttt tcaggtcgtt cttcaggttc agaaaccttc attcatcatg
    50221 actatgttcc agtattacaa acgatcacgg cattttgttt tttcagcatt tattgctttt
    50281 gtttttgtct tgttatgcca gaacacggcg tttgcgcggg cgtcatcgaa tggtgatctg
    50341 ccgacaaaag cggacctgca ggcgcaactt gactcactaa ataaacaaaa agatctttct
    50401 gctcaggaca aactggtgca gcaggatctg acagatacat tagccaccct cgataaaatc
    50461 gatcgcgtaa aagaagagac cgttcagcta cggcaaaaag tcgctgaagc gccggaaaaa
    50521 atgcgccagg cgaccgcggc gttaacagca cttagcgatg tcgataacga cgaagaaacg
    50581 cgcaaaattc tgagcacgct ttcgttgcgc cagctggaaa ctcgcgttgc ccaggcgctg
    50641 gacgatttgc aaaacgcaca aaacgatctg gcgtcttata acagccagct ggtttcgtta
    50701 cagacgcagc ccgaacgcgt gcaaaatgcg atgtataacg cttcgcagca gctgcaacaa
    50761 attcgcagtc gtctggatgg gactgatgtc ggcgagactg ccttacgtcc cagccagaaa
    50821 gtgttaatgc aggctcagca ggcgttgctg aatgcggaga ttgaccagca gcgtaaaagc
    50881 ctggaaggga ataccgtctt gcaggatacc ttgcaaaagc aacgtgatta cgtgacggcg
    50941 aacagcgccc gtctggagca ccagttgcag ctgttgcaag aagcggtaaa tagcaagcgc
    51001 ctgactttaa ccgaaaaaac ggcgcaggaa gccgtctccc cggatgaagc cgcgcgtatt
    51061 caggctaatc cgctggtgaa gcaggaactg gaaattaacc agcagttaag tcagcgtctg
    51121 attaccgcga ctgaaaacgg taatcagttg atgcagcaaa acattaaagt caaaaactgg
    51181 ctggagcggg cgctgcaatc ggaacgcaat attaaagagc agattgccgt cctgaagggc
    51241 agcctgctgt tgtctcgtat cctttaccag caacaacaaa cgctgccctc ggcggatgaa
    51301 ctggaaaaca tgaccaaccg catcgcggat ttgcgtctcg aacagtttga agttaaccag
    51361 cagcgtgatg cactcttcca gagcgatgcg ttcgtcaaca aactggaaga aggtcacacc
    51421 aacgaagtca acagcgaagt tcacgatgcg ttattgcaag tggttgatat gcgtcgcgaa
    51481 ttgctggatc aactcaacaa acagttgggt aaccagctga tgatggccat taacctgcaa
    51541 atcaaccagc agcagttaat gagtgtgtcg aaaaacctga aatccatcct gactcagcaa
    51601 atcttttggg tgaacagtaa ccgtccaatg gactgggact ggatcaaagc gttcccgcaa
    51661 agcctgaaag atgaatttaa gtcgatgaaa attacggtga actgggaaaa agcctggccc
    51721 gccgttttta tcgctttcct cgctggtttg ccgctgctgt tgattgccgg gctgatccac
    51781 tggcgtctgg ggtggctgaa agcgtatcaa caaaaactgg cttccgctgt gggttccctg
    51841 cgtaacgaca gccagctcaa cacaccaaaa gcgatcctta tcgacctgat ccgtgcactg
    51901 ccggtgtgcc tgattattct cgcggttggc ctgattctgt tgaccatgca gctcaacatc
    51961 agcgaattgc tatggtcgtt cagcaaaaaa ctggcgattt tctggctggt gtttggcctg
    52021 tgctggaagg tactggagaa aaacggcgtt gccgtgcgtc actttggtat gccggaacag
    52081 cagaccagcc actggcgtcg gcaaattgtc cgcatcagtc tcgcgttgct gcctatccat
    52141 ttctggtctg tggtggcaga actttccccg ctgcatctga tggatgatgt gctggggcag
    52201 gcgatgattt tcttcaacct gctgctgatt gccttcctgg tatggccgat gtgccgcgaa
    52261 agctggcgtg ataaagagtc gcacaccatg cgactggtca ccattaccgt gctgtcgatt
    52321 atcccgattg ctttgatggt gctgaccgcc acgggttatt tctacaccac gctgcgcctc
    52381 tccgggcgtt ggattgaaac cgtttatctg gtgatcatct ggaacttgct ctaccagacg
    52441 gtattgcgcg gcttaagcgt agcggcgcgg cgtatcgcct ggcgtcgtgc gctggcgcgt
    52501 cggcagaatc tggtgaaaga gggcgcagaa ggtgccgaac cgccggaaga acccaccatt
    52561 gcactggaac aggttaacca gcagacgctg cgtattacca tgttgctgat gtttgcgctg
    52621 ttcggtgtca tgttctgggc aatttggtcc gatttgatca ccgtgttcag ctatctcgac
    52681 agcatcacgc tctggcatta caacggcact gaagctggcg ctgcggtggt gaaaaacgtc
    52741 accatgggca gtctgttgtt tgcgattatc gcctcaatgg tggcctgggc gttgattcgc
    52801 aacctgcctg gtttactgga agtgctggtg ctctcgcgac tgaatatgcg ccagggcgcg
    52861 tcgtatgcca ttactaccat ccttaactac atcattattg ccgttggtgc gatgacggtg
    52921 ttcggatcgt tgggcgtctc ttgggataaa ctccagtggc tggcagcagc attatccgta
    52981 ggtcttggtt ttggtttaca agaaattttc ggtaacttcg tctccggctt gatcattctg
    53041 ttcgaacgtc cggtgcgtat tggcgatacg gtaaccattg gtagcttctc ggggacggta
    53101 agtaagatcc gtattcgtgc gacaacgatt accgatttcg atcgaaaaga agtgatcatc
    53161 ccgaacaaag cgtttgttac cgagcgtctg atcaactggt cgttgactga cactactacg
    53221 cgtctggtga tccgtctcgg cgtggcctat ggctccgatc tggaaaaagt gcgtaaagtg
    53281 ttactgaagg cggcgactga gcacccaagg gtgatgcacg aaccaatgcc ggaagtcttc
    53341 tttacggcgt ttggtgccag cacgttggat catgagctgc gtctgtatgt gcgtgaactg
    53401 cgtgaccgta gccgtactgt cgatgagctg aaccgtacta tcgatcagct gtgccgtgaa
    53461 aacgacatca acattgcctt taaccagctt gaagtgcatc tgcacaacga gaagggcgat
    53521 gaggtgacgg aagtcaaacg cgactacaaa ggcgacgatc cgacgccagc ggtagggtaa
    53581 taacgaaagg gggcgacatt ttgttgcccc gagattgcta acaaagtgcg cgttgttcat
    53641 gccggatgcg gcgtgaccgc cttatccggc ctacgaaacc gcaaaaattc aatatattgc
    53701 aggatcggtg taggcctgat aagcgtagcg catcaggcag ttttgcgttt gcccgcaacc
    53761 ttaggggaca tttagccacc ccatttattt ctcacttttc cgcctcatca tcgcgcgtta
    53821 atttcttttc ataatcgcgt tttacaatat ccagcgcgcg cagcacggtg cgggctggga
    53881 tctgattttc ttccagcagc acaattaaat cgacggccag tttgacatcg tcgggggcat
    53941 tttccagtga catactctct ccattgctag cgggttaaac gcgctaacct gttttcgatt
    54001 ttttccagcg catggcgaca ccgcgccagg cgcgcttcat aggcttccac ttcacgatgc
    54061 agcgtttgct gttccacgag atcggtcacc cgcgccaggc gggctctgcg ttcggcaacc
    54121 atctcacgta gccgccgctc aaaatcctga tgctgaatac gtttacgctg ccagcgggca
    54181 attttgggtg gtgcgctatc ccactcgcgt agcgaccagg ctgtggcttc acgcgctata
    54241 gcctccagtt gcgccgccag atgttctgcc agccaggcga cttgcggcag ttgctgttgc
    54301 tcaactgcat gacgaagcgc agccagatta tcgcccgcct catcgagaca agcctgcagt
    54361 gttgtcgcac gggtctgaaa aaggtgcctg tcaaagcggg cacttagcgt ggcaaattgc
    54421 gctaccgggg cacaacgctg gcgcagcgtg gcgagctgtc cttccagttt ttccagcagc
    54481 agggcggttt tcacgataag acctcaatga aaatgataat tgttatgcta gagtagccac
    54541 tctgtaagct gacaacactc aatatgcaac gaatcatttt aatcatcatt ggctggctgg
    54601 cggtagtgct gggtacgctg ggcgtggtat taccggtatt accgacgacg ccgtttatcc
    54661 tgctagcggc ctggtgcttt gcccgttctt ccccgcgctt tcacgcctgg ttgctgtacc
    54721 gctcatggtt tggcagctat ctacgtttct ggcagaaaca tcatgcgatg ccgcgcggcg
    54781 tcaaaccgcg ggcgattttg cttattttgc tcacgtttgc catttctctg tggttcgtcc
    54841 agatgccatg ggtgcgcatc atgttgctgg taattctcgc ctgtttgctt ttctatatgt
    54901 ggcgaattcc ggtgattgat gaaaagcaag aaaagcactg aagcacaaca atcgcagttg
    54961 caattattgc gtacagccag tacattctgg cgttttcgag cacaggcgca ggcggtcaaa
    55021 ggttaaacaa ctgttacttt tgatacgttt aaaacgcgcc gtgagtacca ccgtaacaag
    55081 caggcataca cttatgaccg cgactgcaca gcagcttgag tatctcaaaa atagcatcaa
    55141 aagcattcag gactacccaa aacccggcat tcttttccgc gatgtcacca gcttactgga
    55201 agacccgaaa gcttacgctc tcagcatcga cttgctggtt gagcgttaca aaaatgcggg
    55261 cattaccaaa gttgtcggca ccgaagcgcg tggcttcttg tttggcgctc cggtagctct
    55321 gggtctgggc gttggctttg taccggtccg taaaccgggc aaactgccgc gtgaaaccat
    55381 cagtgaaact tacgacctgg aatacggcac cgatcagctg gaaatccacg ttgatgccat
    55441 caaaccgggc gacaaagttc tggtggtgga cgatctgctg gcaaccggcg gcactatcga
    55501 agcgaccgtt aaactgatcc gtcgtctggg tggtgaagtg gctgacgctg cgttcattat
    55561 caacctgttc gatctcggcg gcgaacagcg tctcgaaaaa cagggcatta ccagctacag
    55621 ccttgtcccg ttcccgggcc attaattatc gccagtcttg tgctgcccac gctacggaca
    55681 gcacaagatg tgcattcagc ctcgccgttc tgacggggct gtgttagcat taccccttcg
    55741 tgaatccacc ttccagcgtt tcagagcctg ccaatgagtt atcaggtctt agcccgaaaa
    55801 tggcgcccac aaacctttgc tgacgtcgtc ggccaggaac atgtgctgac cgcactggcg
    55861 aacggcttgt cgttagggcg tattcatcat gcttatcttt tttccggcac ccgtggcgtc
    55921 ggaaaaacct ctatcgcccg actgctggcg aaggggctaa actgcgaaac cggcattacc
    55981 gcgacgccgt gcggcgtgtg cgataactgt cgtgaaatcg agcaggggcg ctttgtcgat
    56041 ctgattgaaa tcgacgccgc ctcgcgcacc aaagttgaag acactcgcga cctgctggat
    56101 aacgtccagt acgctccggc gcgtggtcgt ttcaaagttt atctcatcga cgaagtgcat
    56161 atgctgtcgc gtcacagctt taacgcactg ttaaaaactc ttgaagagcc gccggagcac
    56221 gttaagtttc tgctggcgac gaccgatcca cagaaattgc cggtgacgat tttgtcgcgc
    56281 tgtttgcaat ttcatctcaa ggcgctggat gtcgagcaaa ttcgccatca acttgagcac
    56341 atcctcaatg aagaacatat cgctcacgag ccgcgggcgc tgcaattgct ggcgcgcgcc
    56401 gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc aggcgattgc cagcggtgac
    56461 ggccaggttt caacccaggc ggtcagtgcg atgctgggta cgcttgacga cgatcaggcg
    56521 ctgtcgctgg ttgaagcgat ggtcgaggcc aacggcgagc gcgtaatggc gctgattaat
    56581 gaagccgctg cccgtggtat cgagtgggaa gcgttgctgg tggaaatgct cggcctgttg
    56641 catcgtattg cgatggtaca actttcgcct gctgcacttg gcaacgacat ggccgccatc
    56701 gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga cggatattca gctttactat
    56761 cagacgctgt tgattggtcg caaagaatta ccgtatgcgc cggaccgccg catgggcgtt
    56821 gagatgacgc tgctgcgcgc gctggcgttc catccgcgta tgccgctgcc tgagccagaa
    56881 gtgccacgcc agtcctttgc acccgtcgcg ccaacggcag taatgacgcc aacccaggtg
    56941 ccgccgcaac cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc
    57001 agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa
    57061 aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga
    57121 ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg
    57181 ccagccaaaa aagacgcgta tcgctggaag gcgaccactc cagtgatgca gcaaaaagaa
    57241 gtggtcgcca caccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg
    57301 gcggcgaagc tggcggcaga agccattgag cgcgacgcgt gggcggcaca ggtgagccaa
    57361 ctttcgctac caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac
    57421 aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggtgcacag
    57481 caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa cggttgaact gactatcgtt
    57541 gaagatgata atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa
    57601 aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcga
    57661 ttcttcgatg cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt
    57721 acgttcgtca tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg
    57781 taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat
    57841 cgcgcagctg gaagtcaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg
    57901 tgcacacaac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat
    57961 gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca
    58021 gaaagaaaaa atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc
    58081 gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg
    58141 ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc
    58201 ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc
    58261 gattgccgca ccttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag
    58321 gaaaacggtc aaatctgcgt ggtggagagt ccggcggaca tttacgccat tgagcagacg
    58381 gggcagtttt caggtcgtta ttttgtgttg atgggacatc tgtcaccgct ggacggcatc
    58441 ggtccggatg atatcggcct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact
    58501 gaagtgatcc tcgccaccaa ccctacggtt gaaggtgaag ctaccgctaa ctacattgcc
    58561 gagctttgcg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc
    58621 ggcgagctgg aaatggtcga tggcaccacg ctgtcacact cccttgccgg gcgtcataag
    58681 attcgttttt aagcaaacga gagcaggatc acctgctctc gcttgaaatt attctccctt
    58741 gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta
    58801 catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat
    58861 gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc
    58921 cgatgcggcg gacaagttgc gtttccgtgc gctctctaac ccggacctgt acgaaggtga
    58981 tggcgaactg cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga
    59041 taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc
    59101 cggtaccaaa tcattcctcg aatcgctggg ttctgaccag gcgaaagaca gccagctgat
    59161 cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg
    59221 tactcgcgcg gcaggcgaaa aaccagaaaa tggcgtgttc tgggaatcgg ctggcgaagg
    59281 tgaatacacc gttgccgaca tcaccaaaga agatcgtggt actgaaatca ccctgcatct
    59341 gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg cgttccatca tcagcaaata
    59401 ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag atggcgaaac
    59461 cgttatctcc tgggaaaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga
    59521 aatcaccgat gaagagtaca aagagttcta caaacacatc gcccacgact ttaacgatcc
    59581 gctgacctgg agccacaacc gtgttgaagg taagcaggag tacaccagcc tgctgtacat
    59641 tccgtcccag gctccgtggg atatgtggaa ccgcgatcat aaacacggcc tgaaactgta
    59701 cgttcaacgc gtgtttatca tggacgacgc tgaacagttt atgccgaact atctgcgctt
    59761 cgtgcgtggc ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca
    59821 ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct
    59881 ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct
    59941 ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct
    60001 gcgttttgct tctactcata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt
    60061 ttcccgcatg aaagaagggc aggagaaaat ctactacatc accgcagaca gctatgcggc
    60121 agcgaagagc agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct
    60181 ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt
    60241 ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag
    60301 cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgtgtgaaag ccctgctcgg
    60361 cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgattgtctc
    60421 tactgatgcg gacgaaatga gcactcagat ggcgaaactg tttgccgcag cgggccagaa
    60481 agtgccggaa gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc
    60541 ggcagatact gaagatgaag cgaagttcag cgagtgggta gaactgctgc tggatcaggc
    60601 actgctggca gaacgcggca cgctggaaga tccgaacctg tttattcgtc gtatgaacca
    60661 gctgctggtt tcctgatgta atgccggatg accttcgtgt catccggcat ttttcttttc
    60721 atcatctgca ctttccgcaa attatctcgc cattaaccgt ttcagcccct ggtgcctttc
    60781 ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt tcaaaaaaat cgacacattt
    60841 taaggggatt ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg ggaaagggac
    60901 tcaggctcag ttcatcatgg agaaatatgg tattccgcaa atctccactg gcgatatgct
    60961 gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca ttatggatgc
    61021 tggcaaactg gttaccgacg aactggtgat cgcgctggtt aaagagcgca ttgctcagga
    61081 agactgccgt aatggtttcc tgttggacgg cttcccgcgt accattccgc aggcagacgc
    61141 gatgaaagaa gcgggcatca atgttgatta cgttctggaa ttcgacgtac cggacgaact
    61201 gatcgttgac cgtatcgtcg gtcgccgcgt tcacgcgccg tctggtcgtg tttatcacgt
    61261 taaattcaat ccgccgaaag tagaaggtaa agacgacgtt accggtgaag aactgactac
    61321 ccgtaaagac gatcaggaag aaaccgtacg taaacgtctg gttgaatacc atcagatgac
    61381 agcaccgctg atcggctact actccaaaga agcggaagcg ggtaatacca aatacgcgaa
    61441 agttgacggc accaagccgg ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta
    61501 atcaagccgc agcagatctg ctttcgggca ggtctgctca tccacctcag gcaatacctc
    61561 tcacagcaat tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga
    61621 taagaggcgg taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc
    61681 gatgccccca cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc
    61741 gtggttgata cctcacggtt gttatggtgg ccattgctgc gcggcgtgat tttgccgctg
    61801 cgctcgccgc gtgtggcgaa gctgtatgcc tctgtctgga tggaaggtgg ctcgccgctg
    61861 atggtttaca gccgccagca acagcaggcg ctggcacaac gtttaccgga gacgcccgta
    61921 gcgctgggaa tgagctacgg ctcgccatca ctggaaagcg ccgtagatga actactggca
    61981 gagcatgtag atcatattgt ggtgctgccg ctttatccgc aattctcctg ttcaacggtc
    62041 ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata
    62101 tcgtttatac gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta
    62161 cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt
    62221 ccccagcgtt atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc
    62281 gaactggctt ccgcactggg gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc
    62341 tttggccggg aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa
    62401 aaaggcgtag gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg
    62461 ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg gtgcgggcgg gaaaaaatat
    62521 gaatatattc cggcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt
    62581 gccgcgtatc gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg
    62641 gcggttttca tcatccgtga ataatgcaaa aaggcgtgca gcgtgcctgg gtagagtttg
    62701 aactcacagg gctgctgatg cgccgctaac gtctggtaaa gcagacggct gtcatccagc
    62761 agcggatcga actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta
    62821 ttaaacagac agtaatacgg cgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca
    62881 tacatctgca aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc
    62941 acggaatccc gtaatccgta aagcccatac cacagcaaaa cgcccgcaac tttaccgcaa
    63001 tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa
    63061 tcaccggcaa agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    63121 taacaacagg cagccacaat ttcctctatc gcttgcggaa aacgcgcttc aggtgaaagg
    63181 gtgtaatcaa tgccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    63241 tcgtgggtat cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgct
    63301 gggctatctg gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc
    63361 atgtaagctc tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg
    63421 taatactgtc gttgctcagc aatcgttccc gttgcgggcc agggcggtaa atccggctga
    63481 agagtattca caacggtctt catttcagca gaaataaggt ccagaacagg taatttgttt
    63541 tccggcttca tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa
    63601 aatgcgatcc cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg
    63661 tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa
    63721 cgtaaatcca aacattactt ccccgtaaat gcacgcgatc cgctgcttca gcaattccag
    63781 ccagaaaacg aaaccagcgc cgcctgggta gtgggtatcg atcaaacgct ggtcgatatt
    63841 gaagcgaaag tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg
    63901 gtgattgagg atgatgtagc cgaagcgctt tatcaggaac taaaacagaa aaacctgatt
    63961 acccatcagt ttgcgggtgg caccattggt aacaccatgc ataactactc ggtgctcgcg
    64021 gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagctatgcc
    64081 tatcgttacc tgtgtaacac ctccagccgt accgatctta actatctaca aggcgtggat
    64141 ggcccgattg gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc
    64201 agccctggcc acatgaacca gctgcgggct gaaagtattc cggaagatgt gattgccgga
    64261 gcctcggctc tggttctcac ctcttatctg gtgcgttgca agccgggtga acccatgccg
    64321 gaagcaacca tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg
    64381 ctgggcacca agtttgtcat tgcagagaat ccgcagtggt ggcagcaatt cctcaaagac
    64441 cacgtttcta tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat
    64501 ccgttgttag catctgacaa ggcactggac tgggttgatc tggtgctgtg caccgcaggg
    64561 ccaatcggct tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat
    64621 ccgttgctgc cgggcgctat agccgaattc aaccagtatg agtttagccg cgccatgcgc
    64681 cacaaggatt gtcagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg
    64741 ccggaaaaaa tcatgaacac caacggagca ggggatggcg cactggcagc gttgctgcat
    64801 gacattaccg ccaacagcta ccaccgtagc aacgtaccaa actccagcaa acataaattc
    64861 acctggttaa cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat
    64921 caggtactga accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc
    64981 ctggaagagt cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt
    65041 cacgccgcat ctggcatttt gctctcaatg cctgatgcga cgctggcgcg tcttatcagg
    65101 cctacatatc cgcgcctatt atccggcaac caccgtttac cccgtcacca cctcacccgc
    65161 cggtggcgtt tccagcagtt ccagcatagt acgggcaatt tcacgttcac ccatcactac
    65221 ctgattcgcg ccacgttcgg tgatatacgc cacttcatcg tcataatggg cgcgggcaat
    65281 aatctcaata tccggatttt tcgcgcgggc agatgccaca atctcgcctg cttcataacc
    65341 gttgggaatc gtcaggatca gccattttgc acattccaga tgcgccagtt gcataatttc
    65401 ttcgttcgcc gcattgccca atacagcgcg gaccccacgc tctcgcagct catcaacacg
    65461 ggttcgtgac gtctcaatca ccaccagcgg aatatcagag gcgagcaatt tctcccccag
    65521 caggctgcct acacgaccgt aaccaaccag tagcgcatgg ttgcaaatat ccactgggat
    65581 ctgcttctct tcttcgattg cctcttccag cgtctgctct tccagcgttt cggtcttcgc
    65641 cagatatttc tccagcagcg caaacaggac cgggttgagc ataatcgaca aaatcgcacc
    65701 tgccagtacc aggttctgcc cggcttgcgg caacagatcc agcgccatgc ccagtcccgc
    65761 caggataaac gcgaactcac caatttgcgc caggctggcg gcgatggtta atgccgtacg
    65821 ttgggagtga ccaaacagtc gcaccaggaa aaatgcggct aacgacttac caaacagaat
    65881 aatcgccagc gtcgccagca ctgccagcgg ttgctgaatc agaattaacg gatcaaacaa
    65941 catcccgacg gagacaaaaa acagcaccgc aaacgcgtcg cgcaatggca gcgtatcgtg
    66001 ggcggcacgg tgactcagtt cagactcgtt cagtaccatc ccggcaaaga acgcaccgag
    66061 tgcaaaggag acatcaaaca gctctaccgc accaaaggca atccctaacg ccagcgccag
    66121 caccgacaag gtaaacagct cgcgagaacc ggttgccgcg ctgcgtgcca taatccacgg
    66181 caccagacgg cgacctacca gcatcataat ggcgataaac gcgatcactt tgccgatggt
    66241 gatccccata tcgactgcaa gagtggcaaa gcccacatcg ccctgttcca tcattcctgc
    66301 cactgcgggc agcaacacca gcgtcagaac cattaccagg tcttccacaa tcaaccaacc
    66361 gatggcgatt tgccctcgat gactgtcaat taattgccgt tcttcaagtg cgcgtagtaa
    66421 caccacggta ctggcggtgg aaagacataa accgaacacg ataccggtca ttaacgacca
    66481 gcccagcacg gcagagagcg ccatacccag cagcgtcgcc acggctatct gggcgatcgc
    66541 accgggaatg gcgatggcct ttaccgccat caaatccttg agcgaaaaat gcaaaccgac
    66601 gccgaacatc agcaaaataa cgcccagttc agccagttcc ggcgcaagct tggtatcggc
    66661 aacaaagccc ggagtgaatg gtcctgccag cacacccgct aacagatatc ccaccagagg
    66721 agaaatacgt agtttattgg ccagcatgcc gaggataaag gcgagcacaa ggccgccaac
    66781 aatggtggtg ataagcgggg tggcgtgatg cattccgtct ccttttcctg gtggttattg
    66841 tccatttttg gccgggaaaa ccaaaattac aggtaatagt ttatgacaat
//
LOCUS       DAZFFG010000012        66763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFFG010000012 DAZFFG010000000
VERSION     DAZFFG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.012"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            125..1099
                     /gene="hemB"
                     /locus_tag="ACHJ7J_001031"
     CDS             125..1099
                     /gene="hemB"
                     /locus_tag="ACHJ7J_001031"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO4999300.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1205..2056)
                     /gene="tauD"
                     /locus_tag="ACHJ7J_001032"
     CDS             complement(1205..2056)
                     /gene="tauD"
                     /locus_tag="ACHJ7J_001032"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO4999301.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPLQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLMSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWHEAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVLWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2053..2880)
                     /gene="tauC"
                     /locus_tag="ACHJ7J_001033"
     CDS             complement(2053..2880)
                     /gene="tauC"
                     /locus_tag="ACHJ7J_001033"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO4999302.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2877..3644)
                     /gene="tauB"
                     /locus_tag="ACHJ7J_001034"
     CDS             complement(2877..3644)
                     /gene="tauB"
                     /locus_tag="ACHJ7J_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO4999303.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3657..4619)
                     /gene="tauA"
                     /locus_tag="ACHJ7J_001035"
     CDS             complement(3657..4619)
                     /gene="tauA"
                     /locus_tag="ACHJ7J_001035"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4999304.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRRDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            4922..5551
                     /locus_tag="ACHJ7J_001036"
     CDS             4922..5551
                     /locus_tag="ACHJ7J_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO4999305.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            5551..7092
                     /locus_tag="ACHJ7J_001037"
     CDS             5551..7092
                     /locus_tag="ACHJ7J_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4999306.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            7165..8481
                     /gene="uhpC"
                     /locus_tag="ACHJ7J_001038"
     CDS             7165..8481
                     /gene="uhpC"
                     /locus_tag="ACHJ7J_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4999307.1"
                     /translation="METTMHARSAREIDQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            8478..9509
                     /locus_tag="ACHJ7J_001039"
     CDS             8478..9509
                     /locus_tag="ACHJ7J_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4999308.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            9578..11656
                     /locus_tag="ACHJ7J_001040"
     CDS             9578..11656
                     /locus_tag="ACHJ7J_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4999309.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            11668..12714
                     /gene="fbpC"
                     /locus_tag="ACHJ7J_001041"
     CDS             11668..12714
                     /gene="fbpC"
                     /locus_tag="ACHJ7J_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO4999310.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            12874..13149
                     /gene="frmR"
                     /locus_tag="ACHJ7J_001042"
     CDS             12874..13149
                     /gene="frmR"
                     /locus_tag="ACHJ7J_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO4999311.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            13184..14293
                     /gene="frmA"
                     /locus_tag="ACHJ7J_001043"
     CDS             13184..14293
                     /gene="frmA"
                     /locus_tag="ACHJ7J_001043"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO4999312.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            14386..15219
                     /gene="fghA"
                     /locus_tag="ACHJ7J_001044"
     CDS             14386..15219
                     /gene="fghA"
                     /locus_tag="ACHJ7J_001044"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4999313.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYSEQLRTQNLEKVCQEMNIKALIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(15545..16084)
                     /gene="yaiL"
                     /locus_tag="ACHJ7J_001045"
     CDS             complement(15545..16084)
                     /gene="yaiL"
                     /locus_tag="ACHJ7J_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO4999314.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(16186..17397)
                     /gene="mhpT"
                     /locus_tag="ACHJ7J_001046"
     CDS             complement(16186..17397)
                     /gene="mhpT"
                     /locus_tag="ACHJ7J_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO4999315.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(17977..18990)
                     /gene="mhpE"
                     /locus_tag="ACHJ7J_001047"
     CDS             complement(17977..18990)
                     /gene="mhpE"
                     /locus_tag="ACHJ7J_001047"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO4999316.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCEAAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(18987..19937)
                     /gene="mhpF"
                     /locus_tag="ACHJ7J_001048"
     CDS             complement(18987..19937)
                     /gene="mhpF"
                     /locus_tag="ACHJ7J_001048"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO4999317.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLDANVEQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(19934..20743)
                     /gene="mhpD"
                     /locus_tag="ACHJ7J_001049"
     CDS             complement(19934..20743)
                     /gene="mhpD"
                     /locus_tag="ACHJ7J_001049"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO4999318.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIENAKAAYAIQRI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGYPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(20753..21619)
                     /gene="mhpC"
                     /locus_tag="ACHJ7J_001050"
     CDS             complement(20753..21619)
                     /gene="mhpC"
                     /locus_tag="ACHJ7J_001050"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO4999319.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            complement(21637..22581)
                     /gene="mhpB"
                     /locus_tag="ACHJ7J_001051"
     CDS             complement(21637..22581)
                     /gene="mhpB"
                     /locus_tag="ACHJ7J_001051"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO4999320.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(22583..24247)
                     /gene="mhpA"
                     /locus_tag="ACHJ7J_001052"
     CDS             complement(22583..24247)
                     /gene="mhpA"
                     /locus_tag="ACHJ7J_001052"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO4999321.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            24438..25271
                     /gene="mhpR"
                     /locus_tag="ACHJ7J_001053"
     CDS             24438..25271
                     /gene="mhpR"
                     /locus_tag="ACHJ7J_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO4999322.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            25386..26162
                     /locus_tag="ACHJ7J_001054"
     CDS             25386..26162
                     /locus_tag="ACHJ7J_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO4999323.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKPIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIVA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            26182..27135
                     /locus_tag="ACHJ7J_001055"
     CDS             26182..27135
                     /locus_tag="ACHJ7J_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFO4999324.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKHFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            27172..28254
                     /gene="lacI"
                     /locus_tag="ACHJ7J_001056"
     CDS             27172..28254
                     /gene="lacI"
                     /locus_tag="ACHJ7J_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO4999325.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEVAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPTLFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            28377..31451
                     /gene="lacZ"
                     /locus_tag="ACHJ7J_001057"
     CDS             28377..31451
                     /gene="lacZ"
                     /locus_tag="ACHJ7J_001057"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO4999326.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFSAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPMNVAPQGKQVIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQVAITVDVEVASNTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            31503..32756
                     /gene="lacY"
                     /locus_tag="ACHJ7J_001058"
     CDS             31503..32756
                     /gene="lacY"
                     /locus_tag="ACHJ7J_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO4999327.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            32822..33433
                     /gene="lacA"
                     /locus_tag="ACHJ7J_001059"
     CDS             32822..33433
                     /gene="lacA"
                     /locus_tag="ACHJ7J_001059"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO4999328.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSDV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(33536..34717)
                     /gene="cynX"
                     /locus_tag="ACHJ7J_001060"
     CDS             complement(33536..34717)
                     /gene="cynX"
                     /locus_tag="ACHJ7J_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO4999329.1"
                     /translation="MRNHQKTFTILLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGLYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLLLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(34723..35193)
                     /gene="cynS"
                     /locus_tag="ACHJ7J_001061"
     CDS             complement(34723..35193)
                     /gene="cynS"
                     /locus_tag="ACHJ7J_001061"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO4999330.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINLKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(35212..35883)
                     /gene="cynT"
                     /locus_tag="ACHJ7J_001062"
     CDS             complement(35212..35883)
                     /gene="cynT"
                     /locus_tag="ACHJ7J_001062"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO4999331.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     AQPYF"
     gene            35992..36891
                     /gene="cynR"
                     /locus_tag="ACHJ7J_001063"
     CDS             35992..36891
                     /gene="cynR"
                     /locus_tag="ACHJ7J_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO4999332.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAAVHSPELEAIPL
                     LKESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(36893..36984)
                     /locus_tag="ACHJ7J_001064"
                     /pseudo
     CDS             complement(36893..36984)
                     /locus_tag="ACHJ7J_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37024..38307)
                     /gene="codA"
                     /locus_tag="ACHJ7J_001065"
     CDS             complement(37024..38307)
                     /gene="codA"
                     /locus_tag="ACHJ7J_001065"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO4999333.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYWQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(38297..39556)
                     /gene="codB"
                     /locus_tag="ACHJ7J_001066"
     CDS             complement(38297..39556)
                     /gene="codB"
                     /locus_tag="ACHJ7J_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO4999334.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(40262..42148)
                     /gene="prpE"
                     /locus_tag="ACHJ7J_001067"
     CDS             complement(40262..42148)
                     /gene="prpE"
                     /locus_tag="ACHJ7J_001067"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO4999335.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNLPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFALLHHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(42188..43639)
                     /gene="prpD"
                     /locus_tag="ACHJ7J_001068"
     CDS             complement(42188..43639)
                     /gene="prpD"
                     /locus_tag="ACHJ7J_001068"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO4999336.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            complement(43673..44842)
                     /gene="prpC"
                     /locus_tag="ACHJ7J_001069"
     CDS             complement(43673..44842)
                     /gene="prpC"
                     /locus_tag="ACHJ7J_001069"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO4999337.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(45188..46078)
                     /gene="prpB"
                     /locus_tag="ACHJ7J_001070"
     CDS             complement(45188..46078)
                     /gene="prpB"
                     /locus_tag="ACHJ7J_001070"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO4999338.1"
                     /translation="MSLHSPGKVFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            46317..47903
                     /gene="prpR"
                     /locus_tag="ACHJ7J_001071"
     CDS             46317..47903
                     /gene="prpR"
                     /locus_tag="ACHJ7J_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO4999339.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            complement(48004..48279)
                     /gene="yahO"
                     /locus_tag="ACHJ7J_001072"
     CDS             complement(48004..48279)
                     /gene="yahO"
                     /locus_tag="ACHJ7J_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO4999340.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            48426..49097
                     /gene="yahN"
                     /locus_tag="ACHJ7J_001073"
     CDS             48426..49097
                     /gene="yahN"
                     /locus_tag="ACHJ7J_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO4999341.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(49114..49359)
                     /gene="yahM"
                     /locus_tag="ACHJ7J_001074"
     CDS             complement(49114..49359)
                     /gene="yahM"
                     /locus_tag="ACHJ7J_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO4999342.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(49772..50587)
                     /gene="yahL"
                     /locus_tag="ACHJ7J_001075"
     CDS             complement(49772..50587)
                     /gene="yahL"
                     /locus_tag="ACHJ7J_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO4999343.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(50830..51879)
                     /gene="yahK"
                     /locus_tag="ACHJ7J_001076"
     CDS             complement(50830..51879)
                     /gene="yahK"
                     /locus_tag="ACHJ7J_001076"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO4999344.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHALGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(51966..52040)
                     /locus_tag="ACHJ7J_001077"
                     /pseudo
     CDS             complement(51966..52040)
                     /locus_tag="ACHJ7J_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(52256..53638)
                     /gene="yahJ"
                     /locus_tag="ACHJ7J_001078"
     CDS             complement(52256..53638)
                     /gene="yahJ"
                     /locus_tag="ACHJ7J_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO4999345.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(53648..54598)
                     /gene="yahI"
                     /locus_tag="ACHJ7J_001079"
     CDS             complement(53648..54598)
                     /gene="yahI"
                     /locus_tag="ACHJ7J_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO4999346.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(54674..54994)
                     /gene="yahH"
                     /locus_tag="ACHJ7J_001080"
     CDS             complement(54674..54994)
                     /gene="yahH"
                     /locus_tag="ACHJ7J_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
                     /protein_id="HFO4999347.1"
                     /translation="MNALSGLQVAQEFVGLISVAHQAFVTIAGCGVNALSGLRMAQES
                     VGLISVAHQAFVTIAGCGVNALSGPRMAREFVGLISVAHQAFVTIAGCGVDALSGLRV
                     AREL"
     gene            complement(55020..56438)
                     /gene="yahG"
                     /locus_tag="ACHJ7J_001081"
     CDS             complement(55020..56438)
                     /gene="yahG"
                     /locus_tag="ACHJ7J_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4999348.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGHWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(56438..57985)
                     /gene="fdrA"
                     /locus_tag="ACHJ7J_001082"
     CDS             complement(56438..57985)
                     /gene="fdrA"
                     /locus_tag="ACHJ7J_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4999349.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(57975..58838)
                     /gene="yahE"
                     /locus_tag="ACHJ7J_001083"
     CDS             complement(57975..58838)
                     /gene="yahE"
                     /locus_tag="ACHJ7J_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4999350.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDDQGITVGQHLHIEMSRCQRWLSPTLQMNAE
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHHFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(58878..59483)
                     /gene="yahD"
                     /locus_tag="ACHJ7J_001084"
     CDS             complement(58878..59483)
                     /gene="yahD"
                     /locus_tag="ACHJ7J_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO4999351.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            59741..60238
                     /gene="yahC"
                     /locus_tag="ACHJ7J_001085"
     CDS             59741..60238
                     /gene="yahC"
                     /locus_tag="ACHJ7J_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4999352.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            60330..61262
                     /gene="yahB"
                     /locus_tag="ACHJ7J_001086"
     CDS             60330..61262
                     /gene="yahB"
                     /locus_tag="ACHJ7J_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4999353.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(61304..62392)
                     /gene="pdeL"
                     /locus_tag="ACHJ7J_001087"
     CDS             complement(61304..62392)
                     /gene="pdeL"
                     /locus_tag="ACHJ7J_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4999354.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(62534..66517)
                     /gene="ehaA"
                     /locus_tag="ACHJ7J_001088"
     CDS             complement(62534..66517)
                     /gene="ehaA"
                     /locus_tag="ACHJ7J_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO4999355.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQYLSVNNGGEVNASEKISLGY
                     YDQNSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TDYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGLTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT        15354 a        18279 c        17150 g        15980 t
ORIGIN      
        1 ttacaaagat gtcctgggct ctctttcatt ctcttcttgt caacatcgcg acaactttcg
       61 taaaacatcc ccaccctgct tcaggtatac tatgcccctc gattccacaa acatcaggca
      121 gaccatgaca gatttaatcc aacgccctcg tcgcctgcgc aaatctcctg cgctgcgcgc
      181 tatgtttgaa gagacaacac ttagccttaa cgacctggtg ttgccgatct ttgttgaaga
      241 agaaattgac gactacaaag ccgttgaagc catgccaggc gtgatgcgca ttccagagaa
      301 acatctggcg cgtgaaattg aacgcatcgc caacgccggt attcgttccg tgatgacttt
      361 tggcatctct caccataccg atgaaaccgg cagcgatgcc tggcgggaag atggactggt
      421 ggcgcgtatg tcgcgcatct gcaagcagac agtgccagaa atgattgtca tgtcagacac
      481 ctgtttctgt gaatacactt ctcacggtca ctgcggtgtg ctgtgcgagc atggcgtcga
      541 caacgacgcg actctggaaa atttaggcaa gcaagccgtg gttgcagctg ctgcaggtgc
      601 agacttcatc gccccttccg ccgcgatgga tggccaggta caggcgattc gccaggcgct
      661 ggacgctgct ggctttaaag atacggcgat tatgtcgtat tcgaccaagt tcgcctcctc
      721 cttttatggt ccgttccgtg aagctgccgg aagcgcatta aaaggcgacc gcaaaagcta
      781 tcagatgaac ccaatgaacc gtcgtgaggc gattcgtgag tcactgctgg atgaagccca
      841 gggcgcagac tgcctgatgg ttaaacctgc tggagcgtac ctcgacatcg tgcgtgagct
      901 gcgtgaacgt actgaattgc cgattggcgc gtatcaggtg agcggtgagt acgcgatgat
      961 taagttcgcc gcgctggcgg gtgctataga tgaagagaaa gtggtgctcg aaagcttagg
     1021 ttcgattaag cgtgcaggtg cggatctgat tttcagctac tttgcgctgg atctggctga
     1081 gaagaagatt ctgcgttaat ttgtttcctc tcagaaaggg ggagttaacc tgttgaaagt
     1141 acggtgaacg ccccctcacc ctaaccctct ccccaagggg gcgaggggac cgtccactct
     1201 cgtattaccc cgcccgataa aacggtttat ccccaagaat cgtcgcccga tgcataatcc
     1261 gtcgctgtgg caggtaatcg gcattggcat agtgctgggt cacgcggtta tcccaaatcg
     1321 caatatcatt tggttgccag cgccagagca cctgaaactc cggtttggtg atatgggcaa
     1381 acaaaaaact taacaaggct tcgctctctt tctcgctgac atcaacaatt cgcgtagtaa
     1441 agccttcatt cacaaacagc gcctgtttac cgctcaccgg atgcgttcgc accaccggat
     1501 gtagcaacgg cggatttttc gcaaccgcct cgtgccagcg ttgatgctcc tcctcggttt
     1561 tgcggtattt gtattccggg aacgatttac ggaaatcatg ctccgcacgc aacccgctca
     1621 tcagctggcg gaagggaaca gagagcgcct cataggccgc aataccgctg gtccagagcg
     1681 tatcgccgcc ggtcgaaggt aactctttcg ctgccagaat cgcccctgcg ggtggcgttt
     1741 caataaatgt cacatcggta tgccagttgt cgttatctgg cggattatcg ttatgggtat
     1801 ccagcacgat gatctcgtca accccttcgg catgagggta aacagggtga atatgcagtt
     1861 cgccaaaacg ctgggccagc gcgcgttgct gcagcggcgt aatagcttga tcgcgcagaa
     1921 acaccacctg atggcgcagc accgcatggt aaagctgttc aaactgatta tcgcttaacg
     1981 ggcgcgtcag gtcggcaccc gaaatttgtg cgccaatata cggccccagc ggggtaatac
     2041 tcaaacgttc actcattgta cttctccatg ccagggcgtc aggcggcgct gtaacgcgcg
     2101 caaacccagt tctaaaagaa aggcgataat cgcaatcacc gcgatccccg ccagcaccac
     2161 gtcagtcgcg agaaattcac ccgctgactg aaccataaat cctaaaccgc gcgtcgcggc
     2221 aatcagctcc gccgctacca gggtagacca gcccacaccc agaccaatgc gtaatccggt
     2281 gaggatttcc ggcagcgctc cgggcaaaat gacaaaccac agcacctgcg cacggctggc
     2341 acccagcgac tgggcggcac gaatgcgaac ctgctgcgcg cttttcaccc ccgccagcgc
     2401 cgacatcgcc accggtgcaa aaatcgctaa atagatcagt aagatcttcg aggtttcacc
     2461 aataccaaac cagatcacca tcagcggcaa ataagccagc ggcggcaccg gacgataaag
     2521 ctcgattatc ggatccagaa tgccgcgcac cgtcgggctt agccccatgg cgatcccgac
     2581 cggaataccg atcaacaccg ccgcgagtag cgccagcaca atgcgcgtca ggctggctgc
     2641 cagatgctgc cacagcgtgg cgtccataaa tccctgcggt ccggcaatgg tgagcagttt
     2701 tgccagtact tgttgcggcg gcggcaaaaa cagcgggcta atcagttgca gcgccgccac
     2761 cgtccaccat acagtgagta aaaccgctaa cgtgccaatg cttaaggtca cctgacgcga
     2821 gagcggccag cgccatttca gccgccgcga atgcagtttt tcattaatga gcacactcat
     2881 gagaacgcct cccgttgctc aaatacgcgg cttaaaacat attcgcgcat ggcgatgaat
     2941 tgtggatcgg acttgatgct gcggctcgac tctcccgcaa caaagcggcg agcaaagttg
     3001 agcggcagcc gctccagcac acggccaggg ccggatgaaa gcagaaccag ttcagtcgcc
     3061 ataaacaccg cttcttctat atcgtgggta atcaacagca cctgcttgcc cgtctcctgc
     3121 cagagtttca gcagcagggt ttgcatctgg tcgcgggtga aggcgtccag cgcaccaaac
     3181 ggttcgtcga gtaataacag ctggggattc gccgccagcg cgcgggcaat ccccacccgc
     3241 tgacgttgac caccggaaag ctgccagatg tagcgttttt ctgcgccttc cagccccact
     3301 tttttcagca tctggtgcgc gatttccagt cgctgcattt tctctatacc tgccaattgc
     3361 aggccgaacg ccacgttgtc ctgtacattg cgccacggta gtagcccttc attctgaaaa
     3421 actacgccac gctctgctcc cggtccctca atacgcttac ccgccagttg aatgctgcca
     3481 tgctgataag gcacaaaacc ggcaatcaga ttcagcaggg tggttttacc gcagccggac
     3541 ggccccagca ccaccagtag ttcgccgctt tccagcgtca ggttgatatc ttccagtgcc
     3601 ggtttgccgc cataatcggc gtaaagatga gagatttgca gcatctgcgc ctccttttat
     3661 tgcacgaagc gcgaggtaac gtactggctg taatcattcg ctacagccgg gaccttgccc
     3721 tgctctttca aaaactgcgc ggtgtcgatg atcgctttgt tcaccggtcc ggtcagttct
     3781 gccgtttgtt gctgcggcgt cagataggta ttccccttca ccagccccgg aacgtcacct
     3841 tcaggcacgc cgcttaaacg cgccagtttg ctgatgtttt ccggctgttt cagccacacg
     3901 tctgggttag caatgtacgg ttgctgagca tcgatggcgc ttttagcgaa cgctttcacg
     3961 acctcaggat gtttctcggc aaaatctctg cgcaccaccc aaacgtccag cgttggcgcg
     4021 ccccactgtc cgacctgttc agaatcggtc aacaccttgc cgtctttttc cagggcgtta
     4081 accgccggtg cccagacata agcaccatca atatctcccc gctgccaggc agcgataatc
     4141 gcgggcggct gcaggttcac aatctccact tgcccgggtt taatgcccca gtgtttcagc
     4201 gccgccagca ggctgtagtg ggtggtggag ataaacggta cggcgatgcg tttgccaatc
     4261 agatcttccg gtttgctgat agttttcttt accaccagcg cttcggagtt acccaatttt
     4321 gacgccagca agaagacttc aatcggcacc tgttggctgg ctgcaaccgc taacgggctg
     4381 gaaccgaggt tgccgatttg cacgtcgcct gaagccagcg cccgcacgat gctggctccg
     4441 ctgtcaaact tacgccagtc cacggttgct ccgctttctt tagcaaaggt gttgtcggcc
     4501 tgagccactt tcgccggttc ggctgaggtt tgatacgcca cggtgacgtt caccgcctgc
     4561 gcctgaaaag cgatgaatgc cagtgcggca agaagagtgt tacgcgatga aattgccatg
     4621 attgtctgct cccctgtctt gttatgggag cagtatttcg ttgcgggtca gtttgataaa
     4681 ggaattaaaa ggtcttgtta agaacaaagc gtttttagaa aaaaatcggc aatggttagc
     4741 gagtttgcgc attttgcggt tttggcgaac gtatcccgcc ggttcacaag ccagataagc
     4801 tgtgcgcgct ccggcaaatt attgccattt tgttacatcc attccatatc tgtcgctggt
     4861 tgattgccag cgccgcgccg tgatgcatga taggagaatt agataataag agaagaaaag
     4921 tatgattcgg gtagtgctgg tggatgacca tgtggtggtg cgctctggct tcgcacaatt
     4981 actctctctg gaagacgatc tggaggtcat tggtcagtac agtagtgccg cgcaggcatg
     5041 gtcggcatta atccgtgatg acgtcaatgt tgccgtcatt gatattgcca tgccagacga
     5101 aaacggcctc agtttgctta agcgcctgcg tgcacaaaaa ccgcaatttc gcgccattat
     5161 tcttagcatt tatgacgcgc caacctttgt gcaaagcgcg ctggatgctg gtgccagcgg
     5221 gtatttaacc aaacgctgtg ggccggaaga gttagtgcag gcggtgcgct cggtcgggct
     5281 gggcgggcat tatctctgtg ccgatgcgat tcgggcttta cgcggcggcg ggcaacccgc
     5341 ccaggcgctg gaaattctca ccccacgaga acgtgaagtg tttgatttac ttgtcaaagg
     5401 agacagcgtt aaagagatcg cctttaaact cgaactcagt cataaaacgg tacatgtgca
     5461 tcgcgccaat gtgctgggaa aactgaactg ccatagcacc attgaactgg tacattttgc
     5521 gctcgaccac catctgctgg cggggcatta atgtcgcgca atcttcgtca ttgggtgatt
     5581 tcgctgttta ttgtgctggc ctggggaacc ggctggctga tgctgtggac gctcagcttt
     5641 tatctgacca ataacggtca acaggcggtg ttgtttctcc cgcagggagt ttatctcgcc
     5701 cttgttattt tgctctctcg ccgctactgg cctgcgttgg tgctcccccc tttgctgatg
     5761 atgttctggc tccatagtga acagctattg aacggttatc tcatgctggc aacgccggtt
     5821 attagcctgt ttcctgccct ccttgcgcaa aacttctggc accgttttcc actttactgg
     5881 cagcgtctga cgttgctgtt agccactgtc acggcagctt cattgttgaa taccgccctg
     5941 ctctcccctt ttatgtcagg cccaataatg ctgccgggtt tgacctcatt taccggcggc
     6001 gtattactga cgccgtttgt ctatcttatc tttgagtttc tgcgccagca gcatcgctac
     6061 cagctcctgg gactcgatac gcataatccc cccttgcgta cctctttaat catctggtgt
     6121 agcctgtttt ttattattgg tatcggcact cagatggtgc tgtcacctga aattgaacgt
     6181 ctgctgttga tcgtggtctt tttgcccaat gtggtgatgg cgtggaaatt tggctggcaa
     6241 ggtggcgtac tctccgggct tttgggcagc atgatgatca ccatcgcccg ccagattggc
     6301 gtgggcttta gtaacctggt ggagctggag attttcctcg cgacccaggc cttgctggga
     6361 attggcctgg gcattgccat cagtcgccag caacatctgg cgctaaacct gcaccattac
     6421 cgacaacggc tggaggccga gctggcagcg cggcgggcat tggcggaaaa gctgatccac
     6481 accgaggagg acacgcgaaa aaacctagcg cgagagctgc acgacgaaat cggtcagaac
     6541 atcaccgcca ttcagatcca gtcgcaactg gtgaagcggg cgcgtgatcc ggcgcaaatt
     6601 caatccgcag ccagccaaat caatgagctc gcccgccgca ttcatctctc cacccgccaa
     6661 ttgctgcgcc agttgcggcc tcccgcgctt gatgaactga catttcgcga agcgttgttg
     6721 catctgatta atgaatttgc ctttagtgaa cgcggcattc attgccagtt tgcctatcaa
     6781 ctgaactcca caccagaaaa cgaaacggtg cgctttacct tgtatcggtt attacaggag
     6841 ttgctcaata acgtctgtaa acacgccgaa gccagcgaag tgacgattat cctgcgccag
     6901 cagggagagg tattgcatct ggaagtcagc gacaacggtg tggggatcgc cagcgggaaa
     6961 atggccggat ttggcattca ggggatgcgt gagcgcgtca gcgccctggg cggcgactta
     7021 acgctcgaaa aacagcacgg cacgcgggta attgttaact tgcccacatt tttgcaacaa
     7081 atcgccggat aaccaggaaa aagtcttagg tatcccggat tttctctcat cccctttcac
     7141 tttcattttc atatagtcag caacatggag acaaccatgc acgcgcgttc agcgagagaa
     7201 attgatcaat gctaccggac attacgtccg cgattgctga tgtgcatgat tattggctac
     7261 gccgcatttt atctgacgcg aaaaagcgtg aattacgtcc ttccggcgct acaaaccgac
     7321 ctcggactgg ataaaggcga cattggcctg ctgggttcgc tgttttatct cagctacgga
     7381 ttgtcgaaat ttaccgccgg actgtggcat gacagccacg gacaacgcgg ttttatgggc
     7441 gtcggcttgt tcgccaccgg gttattgaac gtggtgtttg cctttggcga atcacttacg
     7501 cttctgcttg tggtctggac actgaacggt ttttttcagg gctggggatg gccaccgtgt
     7561 gcccgcttgc tgacccactg gtactcacgc aatgagcgcg gtttctggtg gggctgctgg
     7621 aatatgtcga tcaacattgg cggagcgatt attccgctga ttagcgcctt tgccgcccac
     7681 tggtggggct ggcaggccgc gatgctgaca ccggggataa tcagcatggc attagggatc
     7741 tggttaacgc tacaactcaa aggcacgccc caggaagagg gattaccgac agttggtcac
     7801 tggcgtcacg atccgctgga gttacgtcag gaacaacaaa gtccgccgat ggggctgtgg
     7861 caaatgttgc gcaccaccat gctgcaaaat ccgctgatct ggctactggg cgtctcttat
     7921 gtactggttt atgtgatccg gattgcgctt aacgactggg gcaatatctg gctgacggaa
     7981 agccacggtg tcaacctgct cagcgccaac gccacggtaa tgctgtttga agtgggcggc
     8041 ttactcggcg cgttatttgc cggatgggga tcggatttgc tgtttagcgg gcaacgagcg
     8101 ccaatgattt tgctgttcac gttggggttg atggtgtcag tcgccgcact gtggctggca
     8161 ccggttcatc actacgctct gctggcagtg tgctttttta ccgtgggctt tttcgtcttt
     8221 ggcccacaaa tgctgattgg acttgcggcg gtggagtgtg ggcacaaagc tgcggcgggt
     8281 tcaatcaccg gatttctcgg cctgttcgct taccttggcg cagcgctggc aggctggccg
     8341 ctgtcgctgg tcatcgaacg ctacggctgg ccgggaatgt tcagtttact ctcggtcgcc
     8401 gcagtactta tgggtttact gctgatgccg ttactgatgg cgggcatcac caccactcac
     8461 gctcgaagga taaaacaatg aaaatgacct ttacctctac tctgattgct tctgccgttg
     8521 cgctggcgac tttaacgggt gccgcgcagg caaaaggccg gctggtggtc tattgcagcg
     8581 ccaccaatga aatgtgtgag gccgaaacca aagcgtttgg cgagaaatat gatgtaaaaa
     8641 cctcgtttat ccgcaacggt tccggaagca ccctggcaaa agtcgatgct gaaaagaaaa
     8701 acccccaggc tgacgtctgg tacggcggca ctttagaccc gcaatcgcag gctggcgaga
     8761 tgggcctgct acaggcgtat aaatccccta accttgagca gatcatggag aaattccgcg
     8821 atccggcaaa agtgaaaggg aatctgtcct ctgccgttta tgtggggatc ctcggttttg
     8881 gcgtaaacac cgatcgtctg aaagagaaaa acctgccggt gccgaagtgc tggaaagatc
     8941 tcaccaaacc ggaatataaa ggcgaaattc aaattgccga tccgcaaagt tccggtactg
     9001 cctacaccgc cctggctact tttgcgcagc tgtgggggga agatcaggcc tttgattatc
     9061 taaaacaact gaacgccaac gtctctcagt acaccaaatc aggcatagcc ccggcacgta
     9121 acgccgcccg tggcgaaacg gcgattggta ttggcttcct gcatgactac tcgctggaaa
     9181 aagaacaggg cgcgccgctg gagttgattt ccccgtgtga aggtacaggc tacgaaattg
     9241 gcggcgtcag cattctgaaa ggcgcgcgca acctcgacaa cgccaaactg tttgtggact
     9301 gggtattatc aaaagaagcg caggaactgg cgtggaagaa agggaagtct tatcagatcc
     9361 tgactaatac caccgccgaa acgtcgccaa attcgctgaa gctcgacgac ctgaaattaa
     9421 tcaactacga catggataaa tatggttcca cggatgttcg taaagcattg attaataaat
     9481 gggtcagcga agtgaagatg ggtaaataaa cccgcattag gcgcatttgc cgggtgcagc
     9541 gcagacgcct gccccggcca accataccgg aactgctatg tcacagacac ttgcactcca
     9601 tcccgtgaaa aagcgggatg cgattttcct ttgggttttg ctgggctggc tggcatttgt
     9661 cctgttgccg agctggagcc tcgattatgg cctgctggaa tccacgagtg atgagatcct
     9721 cggggcgtac ggctggtcgc attttaatat cagctggctg tggtatttac tcccgagcct
     9781 gttgctggtc cgcccgtttc gtcaggccac gcgcgaacag cgtagtcgtc attatttcga
     9841 tgccggctgg gcattgctct gtatggcgtt tatcgtcatc agcgccaccg tggctggacg
     9901 cggtttaggc tacgccgcca tcgtgctgtt tgtcgaacta ggcgcgatta tgacgctggc
     9961 cttaacccgc ctcgaatggc tgggcggcga ccgcttcgtg atcggctcac tgattgcgat
    10021 tgtggcgctg attggcattt ttatcgtctg gccgagcatc gccattttta tcccgatgtt
    10081 caccaacgac gcgggagagc tcgccccgct ggcgtttatg accgtgctct cgcaggcaca
    10141 tattgttcag gtgatcgtca actctatcgt cttgtcgata gcggtgggga taggctgtac
    10201 tttcttcggc ctggtgctgg cgatttatac cacgcgcatc gctaaacgca gcgccattat
    10261 tggtcgcatc ttttccattt tgcccatcgt tacgccaccg tttgtggtcg gcttaggcgt
    10321 gacgctcatg atggggcgct ccggctatgt tacggaattt atggtggagt ggtttgggct
    10381 gaccaacacc aactggctgt acggttttac cgggatctgg ctggcacagg ttctggcatt
    10441 cacgccaatg gcatttatga tcctcgacgg ggccattaaa accattcatc cctcgctgga
    10501 agaggcctcc tacaccctgc gcgccagccg ctggcaaacc tttaacggcg tgtttgtgcc
    10561 gttgttgaag cccgcactcg ccaatgcctt tttaatcgtc gtggtacagt cactggctga
    10621 tttcagtaac ccgctggtac ttggcggtaa cttcgacgta ctggcgacgc aaatttactt
    10681 ctatattacc ggctcgcagc tggattatca ggccgccagc acccttggcg ctttcctgct
    10741 gctgttctcg ctgctggtgt tctgcatcca gtatatgtgg attggtaaac gctcttacgt
    10801 cacggtttcc ggcaaatcct accgtggcga cgtccagccg ctaccggtca ccctggtgtg
    10861 gagcgtggtc gccatacttg cgatatggat tgcctttaac gccctgctct acggcagcat
    10921 tttctacggc agcttcacgg tgaactgggg cgtggactac accctgacgc tggataactt
    10981 tatcaagcta tttggtcagg gaatgagcga cggcgcctgg ccttcactgc tggataccct
    11041 gctctacgcc gggattgccg cccccattac cgctatcttt ggtctgctca tcgcctgggt
    11101 ggtggtgcgt caacaattca aaggcaaaaa gaccatcgag ttcaccacca tgctgtgctt
    11161 tgccgtaccg ggtacggtgg cgggcgtctc gtatattctt gcctttaaca gcgctccggt
    11221 ttatctcacc ggaacggcgg caattgtgat tatctcgatg gtgatgcgta acgttccggt
    11281 gggtattcgc gccgggattg cgggtctggg gcagatcgac aaatcgctgg atgaagcctc
    11341 actcagcctg cgcgcgggtt ccttgcgaac cataacccac attttactgc cgctgctgcg
    11401 tccggccatt ctctcggccc tgatctacag ctttgtgcgg gccatcacga cggtcagcgc
    11461 catcgtcttt ctcgtcacgc cggacacccg cgtggcaacc gcctacattc ttaaccgcgt
    11521 tgaagacggc gaatatggcg tagccatcgc ctatggttcg attctgatcg tggtgatgct
    11581 ggcgattatt ttcctctttg actggctgat cggagaagct cgtatctccc gttctaaagc
    11641 caaaaaccag gcgtaatgga gcgcactatg agtcagaaaa attttgttga actgcgcaac
    11701 gtcactaaac gattcggcag taatacggtt atcgacaata tcaatctcac cattccacaa
    11761 gggcaaatgg tgacgctgct cggtccttcc ggctgtggca aaaccaccat tttgcgtctg
    11821 gttgccgggc tggaaaaacc gagcgaaggg caaattttta ttgatggcga agatgtcacg
    11881 catcgttcca ttcaacagcg cgatatctgc atggtgtttc agtcatacgc tctgttcccg
    11941 catatgtcgc tgggtgaaaa cgttggctac gggctgaaga tgcttggcgt gtcgcgtagc
    12001 gaagtgaaac agcgggtgaa ggaggcgctg gcaatggtgg atctggaagg cttcgaggac
    12061 cgctatgtcg atcagatttc cggtggtcag caacagcgtg tggcactggc ccgcgcgttg
    12121 atcctcaagc caaaggtgct gctgtttgat gagccgttaa gtaacctcga tgccaacctg
    12181 cgccgcagta tgcgcgataa gatccgcgag ctgcaaaagc agtttaatat cacgtcgctc
    12241 tacgtcactc acgatcaaag tgaagctttc gcggtgtccg acactgtgct ggtgatgaat
    12301 aaaggtcaca tcatgcagat tggctcaccg caggatctct atcgtcagcc agcctcccga
    12361 tttatggcaa gttttatggg cgacgccaac ctgttcccgg cgaactttag cgaagagtat
    12421 gtcgatatct acggttatcg cctgccgcgc gcggcgcatt tcccggcgca aggtagcggc
    12481 accgtcggcg ttcgcccgga agccatcacc ttaagcaatc acggcgaaga gagtcagcgt
    12541 tgtgttattc gccatgtcgc ctacatgggg ccgcagtacg aagtgaccgt agagtggcat
    12601 ggacaggaga ttttattaca agtaaacgcc acccgtttac agcccgatat tggtgagcac
    12661 tattacctcg aaatccatcc ttacgggatg tttgttttag cggatgcggc ataaaaatag
    12721 cacataaacg gtccccatct gtcttgaggg accgttcacc tggttaggct tcggacaaca
    12781 attattctcc agtgttatat actatagggg ggtatgtatt gacatatagc atacccccct
    12841 atagtatatt gcgtgcagat gatgaggtgc gaaatgccca gtactccgga agagaagaaa
    12901 aaggtcctta ctcgagttcg tcgtattcgg gggcagattg atgctctgga acggtcgctg
    12961 gagggtgatg ccgaatgccg tgccatactc caacagatcg ctgccgttcg gggcgcggct
    13021 aatgggctga tggcagaagt gcttgaaagc catatccggg aaacgtttga ccgaaatgac
    13081 tgctacagcc gcgaagtcag ccaatccgtt gacgacacta ttgaactggt tcgtgcctat
    13141 cttaaatagc tgaatctatt accatattga ggaagagcga gagatgaaat cacgtgctgc
    13201 cgttgcattt gctcccggta aaccgctgga aatcgttgaa attgacgttg caccaccgaa
    13261 aaaaggtgaa gtgctgatta aagtcaccca taccggcgtt tgccataccg acgcatttac
    13321 cctctccggc gatgacccgg aaggtgtatt cccggtggtt ctcggtcacg aaggggctgg
    13381 cgttgtggtt gaagtcggtg aaggcgtaac cagcgtcaaa ccaggcgacc atgtgatccc
    13441 gctttacacc gcagagtgcg gcgagtgtga gttctgtcgt tctggcaaaa ctaacctctg
    13501 tgttgcggtt cgcgaaaccc agggtaaagg cctgatgcca gacggcacca cccgtttttc
    13561 ttacaacggg cagccgcttt atcactacat ggggtgctct acattcagtg aatacaccgt
    13621 agtcgcggaa gtgtctctgg caaaaattaa tccagaagca aaccatgaac acgtctgcct
    13681 gctgggctgt ggcgtgacca ccggtattgg cgcggtacac aacacagcta aagtccagcc
    13741 aggggattct gttgctgtgt ttggtcttgg cgcgattggt ctggcagtgg ttcagggcgc
    13801 gcgtcaggcg aaagcgggac ggattatcgc tatcgatacc aacccgaaga aattcgatct
    13861 ggcgcgtcgc ttcggtgcca ccgactgcat taacccgaat gactacgaca aaccgattaa
    13921 agatgtcctg ctggatatca acaaatgggg tatcgaccat acctttgaat gcatcggtaa
    13981 tgtcaacgtg atgcgtgcgg cgctggaaag tgcgcaccgc ggctggggtc agtcggtgat
    14041 catcggggtc gcgggttccg gtcaggaaat ctccacccgt ccattccagt tggtcactgg
    14101 tcgcgtatgg aaaggttccg cgtttggcgg cgtgaaaggt cgttcccagt taccgggtat
    14161 ggttgaagat gcgatgaaag gtgatatcga tctggaaccg tttgtcacgc ataccatgag
    14221 cctggatgaa attaatgacg ccttcgacct gatgcatgaa ggcaaatcca ttcgaaccgt
    14281 aattcgttac tgatttcccg ctggtttacc ccgtccactt cagacggggt tcttaatact
    14341 ctccccgggc agccgtccgg gggattaacc gtgagataat gactgatgga actcattgaa
    14401 aaacatgcca gctttggcgg ctggcaaaat gtgtatcggc attattccgc atcactgaaa
    14461 tgtgaaatga atgtcggcgt ctatctccca ccaaaagctg caggtgaaaa attaccggtg
    14521 ctgtactggc tttcgggcct gacctgcaat gagcagaatt tcattactaa atcggggatg
    14581 cagcgttacg cggctgagca caacattatt gttgttgcgc cggacaccag tccgcgaggc
    14641 agtcatgtcg cagatgccga ccgttacgat ctcgggcaag gtgccgggtt ttacctgaac
    14701 gcgacgcaag cgccgtggaa tgaacattac aaaatgtatg actatatccg caacgagctg
    14761 ccggatttag tgatgaatca ttttccggca acggccaaaa agtctatctc tggtcattct
    14821 atgggcggac tgggcgcgct ggtgctggcg ttacgtaacc cagatgaata tgtcagcgtc
    14881 tcggcgtttt cgcccattgt ctccccatcg caagtaccgt ggggacagca agcctttgcc
    14941 gcatatcttg gtgaaaataa agatgcctgg ttggattacg acccggtgag tcttatttca
    15001 caaggtcaac gcgttgcgga aatcatggtt gatcaggggt tgagtgatga tttttactcg
    15061 gaacagctgc ggactcaaaa tcttgaaaag gtctgtcagg agatgaatat caaggcgtta
    15121 atccgttatc acgaaggtta tgatcacagc tattattttg tctccagttt tattggcgag
    15181 catattgcct accacgccaa taaactgaat atgcgttaat aatagtgcac gactgccgga
    15241 tgcggcgtga acgccttatc cgcctacatt tcgcccgcag gccgtaggca tgataagacg
    15301 cgcatagcgt cgcatcaggc atctgcgcac gactgccgga tgcggcgtga acaccttatc
    15361 cggccaacat ttcgcccgta aaccgtaggc ctgataagac gcgcaagcgt cgcatcaggc
    15421 atctgcgctc aactgccgga tgcggcgtaa acgccttatc cggcctacat ttcgcccgtc
    15481 aaccgtaggc ctgataagac gcgcatagcg tcgcatcagg catctgcgca cgacttatct
    15541 atgtttacca catcaaatca tcaggcactt tgaagtcggc atacggatcg tcttcatcct
    15601 gctcttcggc actgagcgcg ctgtgcaaca caatactgct ggcatcacgc tgagcaattt
    15661 tatcggcaac gctggcggga ataatggcgt attcaccttc gctattgtta tcgaccaaca
    15721 agcgggcaat cgccagacga ccattaatca actgcgcctg tgtgagctta tcgacaaaaa
    15781 tcttcttaat cagattgccg tcagtgaagt taaaaccaat atcgccattg gcaatggtga
    15841 ttcggttcat ttcaatgagc tgcttcacct gggctttata ttctttcgcc aacgccgctt
    15901 gtttttgctg ttcgctaagc tgtttatcac gctcaagctg tgccttttta ttttcttcta
    15961 ccgccgcccg agcttcacgc gcctgaacgc gcgatttttt cgccgttctc tcgaccttcg
    16021 ccgctttttt gctggtgact aatcctgctt tgagcaactg ctcttgtaag gtaagttttg
    16081 ccatctttgt ttccaaaccc gctgaataat ttttagcgag tatacctgta aacgccgtgg
    16141 gtgtattggg ggcaagtccc tttcgcccct gcttaataag gcgcgtcagg catcggcaca
    16201 cggctgcatt cttgatctcc gactcatcaa aataaacacc gccaacccag caacaagaat
    16261 acccggtgca gaagcggcca ttacgccgac cgtgccagtg cctaatgcca gcattttccc
    16321 ggccagtaac ggaccgctca tagcccccag acgccctacc gccacggctg ttcccacacc
    16381 tgttgcgcgg atctgcgaac tgtaaaacaa cggtgccagg gcatacaaaa cgctttgccc
    16441 acctgtcgca aacaaccccg cgacaaatcc cgccagcaac ataccgttaa atgacgacac
    16501 cgttccaagc gccagcagcg aagctaacat gccgctataa atcagtagcg acatggttac
    16561 tggacgcagc ttatccatca atgcgcccaa cattaacgtc ccgcttgccg cccccatttg
    16621 cagtgcaaac atcacccctg ccgcctgcga tggctggaat ccttgctcca ccaaaagcag
    16681 cggtagccag ttgatcaaca tgtagaccac cagcagagtg aagaaataac acagccacag
    16741 cagcagcgtc gcggttgccg tttctggcgc aaataacgca cgcagtggtg gcgcagcctg
    16801 tttttcgcca gcgaaaaccg ccgactccgg caaccagcgc attaatagcg gcaccagaat
    16861 caacggcacc acaccaccta cccaaaacac cgtttgccat gctaagtttg cccccgcgaa
    16921 acccagtgtc gccgccagcg ccgcgccaat gggaacaccg caatacatca ggctcactgc
    16981 cgtcccacga aaacgtggac ccgcggcttc agacgtcagg gcgataagat tcggcaacgc
    17041 cgcccccagc ccgacaccgg tcatcagccg cgcaaagacc agtgagggga aatcccaggc
    17101 aatcgccgtt gccagcgaga acaaaccaaa cagcgcaact gagccaatca aaatgcgctt
    17161 gcgaccataa cggtccgcca gcattccgcc aaccaacgcg ccgggtagta aaccgagtat
    17221 tccggcgctg aatatccagc ccatttgcat tttatcgagt gcgaaagcct gggcgatgcc
    17281 acccgccgca atgccagccg cctgaagatc cagcccttcc atcagagcga ccaaaaaaca
    17341 aagcccgatg gtcagcatca ggcgggatga agatgatgaa ggggtacgag tcgacataag
    17401 atgaacctcg ttgcgagtgc aggtgaatcg cgactgatac attgccagcc gcgtaatcag
    17461 aactatttaa tgactgccta cgggcgaaat gtaggccgga taaggcgttc acgccgcatc
    17521 cggcagtcgt gtgcagatgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaaattc
    17581 tcacgccatc cgtaggccgg ataaggcgtt tacgccgcat ccggcagtcg tgcgcagatg
    17641 cctgatgcga cgcttgcgcg tcttatcagg cctacaaatt cccgcgccat ccgtaggccg
    17701 gataaggcgt ttacgccgca tccggcagtc gtgtgcagat gcctgatgcg acgctaacgc
    17761 gtcttatcag gcctacaaat tcccgcgcca tccgtaggcc ggataaggcg tttacgccgc
    17821 atccggcagt cgtgcgcaga tgcctgatgc gacgctaacg cgtcttatca ggcctacaaa
    17881 ttcccgcgcc atccgtaggc cggataaggc gttcacgccg catccggcag tcgtgctggg
    17941 atgggcatac aaaatgcacg ttctcggtag tcatcattat ttgttgttgc gcagatccag
    18001 cgccacgtcg acgatcatat cctcctgacc gccaaccatc cggcgtttgc ccagctcaac
    18061 gagaatatcc accgcgctta agccataacg cgccgccgcc gcttcacagt gacgcaggaa
    18121 gctggagtaa acaccagcgt atcccagcgc cagcgtttcg cgatcgactc gtaccggtcg
    18181 atcctgcaac ggacgcacca ggtcgtcagc ggcatccatt aacgcataga gatcggtccc
    18241 atgctgccaa cccagtttat ccgcggcggc aataaatact tccagcggcg cgttacctgc
    18301 gcccgctccc attccggcga ggctggcgtc gattcggtcg cagccctctt ccaccgccgc
    18361 gatagagttc gccacgccaa gactcaggtt atggtgagcg tgcatgccgg tttgcgtttc
    18421 tggtttcagc actgctttca gggcgcggaa acggtcacgg atatcgctca tgttcatcgc
    18481 accgccagaa tccaccacat aaatacaggt cgcaccgtag ccttccatca gctttgcctg
    18541 cttggcgaga ttctccggcg tggtcatatg gctcatcatc agaaaaccaa cggtgtccat
    18601 tccgagctcg cgggcatact gaatatgctg ggcggaaaca tcagcttcgg tacagtgcgt
    18661 agccacacga accacccgcg cgccagcctg ccaggcattt ttcagatcgt gaatagtgcc
    18721 gattcctggc agcaacaacg tcgcgatttt ggcgtgcttc accacatccg ccgctgcttc
    18781 aatccattca aggtcgctat gtgcgccgaa accatagtta aagctggacc cctgcaaacc
    18841 gtcgccgtgg gccacttcaa tcgaatccac gcgggcatcg tccagtgctt tggcaatctg
    18901 gcgaacgttt tccagcgaat actgatgacg aatggcgtgc ataccgtcac gcaacgtgac
    18961 gtccgagata taaagttttt taccgttcat gccgcttctc ctgccttgcg cgccagtgac
    19021 tgggccattt tttccgctgt cgccagcgca ctggaagtca taatgtcgag gttgcccgca
    19081 taggcaggca gataatgcgc tgcgccttcg acttccagcc agaccgctgt tttcagtccg
    19141 gagaattgcc ccacgcccgg taaattgacc ggtttatcct gcgggataac ttcaaactgc
    19201 acgcgctgtt tcaggcgata tcccggtacg taagcctgca ccgcctcagc catttcatta
    19261 attgaggctt cgatatcatc ttgtgaagct tcgtcgctca atacatacac cgtgtcacgc
    19321 atcatcaacg gtggctctgc tgggttaagc acgataattg ccttcccttt tgccgcgccg
    19381 cccaccactt caatggctcg ggaagtggtt tccgtaaatt catcgatatt ggcacgcgtg
    19441 ccaggtccgg cagatttact ggcgatagaa gcgataattt cggcgtaatg aacacgcgcc
    19501 acgcgtgaaa ctgccgccac cattggaatg gtggcctggc cgccgcaggt gaccatattg
    19561 acgttcagtt gttcgacatt cgcatcaagg ttaaccaccg gcacgcagta agggccgatg
    19621 gcagcaggcg tcaggtcgat taagcgaata tccggtttcg cttcgcgcaa agcggcatcg
    19681 tttttcacat gtgcgcccgc gctggtcgca tcaaatacaa tgtcgatatc agcaaattca
    19741 ggcatgttca tcagcccgat caccccttca tgggtggtgg cgacgcccat acgtctggca
    19801 cgcgccagac cgtcggactg aggatcaata ccgaccatca ccgccatctc cagatgctga
    19861 ccgtgacgca aaattttaat catcagatcg gtgccaatgt tgccagaacc gataatggcg
    19921 actttacgct tactcatgac agacttcctt ttggggctgc gcttgaaaat gtcgcagcaa
    19981 ctgaacctat gccttcaata tgggcttcaa aacgatcgcc cgcattcact gccaccatcg
    20041 gacctaatgc cccggtaaga atgatatctc cggcgcgcag cggttcaccc agactggcca
    20101 ttttgcgtgc cagccagacg gcggcattaa gcggatatcc caggcattcg ctgccgcgcc
    20161 cgctggaaac ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc aggtctaacc
    20221 ccgccggacg ttgcgccgga ccgccgatga catacacccc gcaggaagcg ttatctgcca
    20281 cggtatcgac aaactgaatc gaccagtcgc gaatgcggct ccccaccacc tccagcgccg
    20341 gaagtaccca ttcaatggcg ttatacaatt cgtcgaaggt gatatcggtt gcgggcaaat
    20401 cgcggttcaa caccagtgcg atctccgctt caatgcgggg ttgcagaaca cgggaaaaag
    20461 gaatgatttc gttatcgcca taacacatgt cggcaaataa tgtcccgaaa tccggttgat
    20521 caacgcccag ttgttgctgc actttcggat gtgtcaggcc cactttacgc cccaccacgc
    20581 gacgcccctg cgcaacgtca tgttgcacat tgatgcgctg aatcgcgtag gcggctttag
    20641 cgttttcgat accaatcaga tcgcgcagcg gcgcaatcgc ttcgccctgc tctgcggcgc
    20701 ggcgtaaatc cgccgccagt tgctcaagag tatgcttcgt catgaccgtt ccttaagcac
    20761 gtgcgaggaa attcagcacc agttgattga aagcgtcggc atgttcccac tgcgcccagt
    20821 gaccacagtc gcggaagata tgcagttcag aaccggcaat gccggacagc agacgcagac
    20881 ccgcatccat cggcacaaag cggtcgttgc gcccccagac aatcagggtt tgcgctttga
    20941 tttccgccag acgtgggcca aaatccggga actgtttcgg attagcttcc aggctcttaa
    21001 cgaagttttc caggtgatcg cggcgcgaca gcatattatt caggcgcgct tcaaacaggg
    21061 cgtcggtcaa atcgctggta tcaaaaacga agatatccat catcagcttc aggttttcga
    21121 tagtcggctg acgataaagc tgattcagtc gcttaatacc ttcggttggc atcggcgtaa
    21181 acaaactcat gccgcccgta ccgccgccca tcagcaccag tttgccgacg cgctccggcc
    21241 agttaagggt gaacgccaca gaactatggc cgcccatcga gttgcccaac aggtggattt
    21301 tggcgatatc cagttgatcc accacgcttt tcaggatgcg cgcattaaga tccgatcgtg
    21361 aaccactatt aacgatcgaa tcgctcttgc cccaacccgg acaatccagc aggatcaccc
    21421 gatagcccgc ctctaccagc ggatcgatat tgcggctgaa gttcgcccag ccagtagcac
    21481 ccgggccgga accgtgcagc aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat
    21541 ggatgcgcag cgttttaccc gcttcttcta cattcagaaa acggctggtg gcggcttcgg
    21601 tttgtggctg ataactcatc atcttctcct gcatattcag ttctctgttc tggcgcttaa
    21661 cgagccaaat ccggcaatcc actccgggat tgggcggtaa taacgccctt cgctacgcca
    21721 gttgccaaat gcagaaatag cggcaaaagc ggcgacccag gttttgattt catgtgtcga
    21781 ctttccggca atggcggaaa gctcttcgtt actgacggca tccagttcct gtatgcgtcc
    21841 ctgctccagc aaagtcatga actggttatc ccaaatcggg ttgagcggat gcagagttct
    21901 ctgatcctca acaaacttct cagcggcgct aatcacccgt tgctgacgca attcgcgctc
    21961 actggcgggt aaatctttcc cgctccccaa cagacggtcg cgcatatggg catcggcttt
    22021 cgccagttcg ggcaccggcg gctgatggga aagcccaccg gaacccagga acagcacgcg
    22081 tttattgaga gtgctggtga aacgtccaat ggcttcaccc aacatgcggg tacgctggaa
    22141 accgggcagc ggcgtggcga caccgttgat gaacacaggc agaactggca ccttatccag
    22201 cccaccgagc aggaactcca gcggttgggc gaacccgtgg tccacctgca tacagtaaga
    22261 aacggcaaga tcgatcccgc tcttcatgac ggcatgcgca caggcctccg ccagctccac
    22321 aggcactggc agctctcctg ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc
    22381 taaacagaac ggtggcatca cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag
    22441 caccaccagt tcaggggaga aagccgcaat acgctcgcgg gcgctggcaa tcacgccatt
    22501 gacctcatcg agtacctctt gtgccgggtc gacatacccc accagcggcg agtgggaaag
    22561 acaatgaaga taagcgtgca tatcaggcta ccttttcgac agaaacgtcg gcatcagggc
    22621 gggtcagcgt catcaccgac gccagtttat tcagggtctt gcccagggtt tgcggaatgg
    22681 cggtggcggc aacaaagcga tccgggcgca tcaccaccag cgaagcattg tgttgcgcga
    22741 accagctacg caggcgacct tgcgtatccc ccacgcgtag tacgccgtcg tggttatcct
    22801 gtgcggtatg aatttgcact tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc
    22861 actgctggat ttgctcgtcg ctcatccccc acagtggatt gcatccccag ccaattaccg
    22921 cgaagttcgc gccgatcgcg ttatcgagca gcgtcacgtc gccgttttcc agcgtgactt
    22981 tcggctgaat aaacatcttg ccgaccggag agtgcttcgc ttcgccctca cgtaccagcg
    23041 caccgccgta atattgcggc atcggcttga agcgcatttc gaggaagtag cgttttactg
    23101 gcggcagata attcagcagc caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg
    23161 gcggagccag cacgttgccc gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc
    23221 gacgttcttg ttgataggta tcgagcagcg catcgcgggc tttcccctgg ataaccaacg
    23281 ccagtttcca tgcgaggtta aaggcgtcgc gcatgccact gttatagccc tgcccctgcc
    23341 ataccggcat gatgtgtgcg gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt
    23401 gcgccagtcg cgcgttgtgg gtgtagacac gctggcgaat caattcaaca ttgtccggat
    23461 taggcagcac tttgcttaac agcttgcgca tattttgcgg ctcacgcagc tgttcttcgg
    23521 tttctcctgg catcaccata aattcaaagc gacgtaccgc atgaggtagc gcggcagaaa
    23581 catacgggcg caccggatcg caacacaaat agatatgcgg cgtacttaac ggatcgttgg
    23641 cgatatctac cacaatccac tgatttggcg cagttttacc ttcaaacggc acattcaggg
    23701 tgcgacggac aaaacttgcc ccaccatcac aggccaccag ccactgggct ttgacgattt
    23761 cccgctgccc ttctgccgtt ttcaggtgca aggtcacttc gtcatcttgc tgactgaagg
    23821 cctccagctc gcgggaaaac aagcagcgca cattcggaaa acgcgacacc ccttccagca
    23881 tcaccgcatc gacctgcggc tgaataaagg cgttacggcg cggccagcca aattcatcgg
    23941 tcattggctg aatatcagca aaacagcggc ccttcggggt gagaaaacgc atcgcgtgcc
    24001 acggcgtagt atgcggcaga acattatcga ccaggccgac cgactgcatg gtgcgcagcg
    24061 cctcgtcatc aataccaatc gcacgcgggt agtcgatcaa cttatcgagt ttctccacca
    24121 ccagcacgtc aatgcccatc tggccgagat agttcgccat catcagcccg accggaccgg
    24181 caccagcgat cgccacctga acgctatggt taacagcagg ctggatgtca gggtgttgta
    24241 ttgccatttc agtacctcac gactcggaca aaatgccgtt gcgcgcacag tacagcgcaa
    24301 cttattttgt taaaaacatg taaatgattt tttattgtgc gctcagtata ggaagggtgt
    24361 tttcggctac aatcaaaaca tgcccgaatg tgcaccaggt gcacaacgtt gttttaacta
    24421 tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct
    24481 taacccgcgg tctaatgtta ttaaatatgt taaataaact tgacggcggt gccagcgtcg
    24541 ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgc
    24601 tgcaggaaga gggatatgtc cggcgtagtc cctccgatga tagttttcgt ctgaccatca
    24661 aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc
    24721 cactgctggg cgatctgtta cgcgaagtgg tatggccgac agatgtgtcc acgctggatg
    24781 ttgatgcaat ggtggtacgc gaaaccactc accgtttcag ccgcttatcc tttcaccggg
    24841 caatggtcgg gcggcgttta ccgcttctga aaaccgcctc gggcctgacc tggctggcct
    24901 tttgtccgga acaagaacgc aaggaattaa tcgaaatgtt agcctcccgc cccggtgatg
    24961 actatcaact ggcacgagaa ccgttaaagc tggaagccat tctggcgcgc gcgcgcaaag
    25021 agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg
    25081 taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg
    25141 caatgaccat cgaacaagca gcggaaaagc atcttccggc actacaacgg gtagcaaaac
    25201 agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga agcggcgtgc
    25261 atttacgcta gttacgccaa tgtgtaattt ggcccattct catcgttatt cgcccatttg
    25321 cccacatgca cagtacgggc tgcacgctac gctaatgaca tcaatactgt gatgtggagc
    25381 aaatcatgaa aattacgcaa attcgtaatg ccacccaaat tatccaatac gctggcaaaa
    25441 catttcttat cgacccgtta ctcgcgccta aaggaagtta tccgggtttt gccggaacgg
    25501 cacgcgcaga aatccgcaat ccaacggtag aattaccgtg cgatttaaca acgttactca
    25561 acgttgacgc cctaatcgtg acccatttgc atgaagatca ctgggacgaa acagcctccc
    25621 gcgtcgtccc taaagataaa ccgatttatg tgcaaaatga gcaagatgct gaatcactga
    25681 ataaacaagg atttacgcat cttaccgtac ttaccgacga gactaaattt ggcgatatta
    25741 ctctgcataa aacgacaggg cagcacggct ccgatcgcac ctatgccgac ccggcgatgg
    25801 cgaagcgttt aggcgcagcc tgcggcgttg ttttccagca cccgacagag aaaacgctct
    25861 atctggctgg cgataccctc tggcgtgacg acgtcgaggc tacaatgcta actttccaac
    25921 cagaagtggt tgtgttgaat gccggatttg cgcatgttct ggggcatggc gcgataatta
    25981 tgggagcaga agatattctc aaaacacatt ttaccctccc agaagcgcaa attgtcgcga
    26041 cacatatgga ggccgtaaac cactgtttgc tgacgcgtga tgcgctcaag gaatacgcca
    26101 gagataatca aattatcgaa tttgtgaacg taccagaaga tggtgaaacg ttgacattct
    26161 aattcggaga taaagagtgt tatgcgcccg ctaactgttg ccattatcgc cgttgccggt
    26221 tttagtcctt ttcacctttc cgtaccgttt atcgtattta gcgaaaaaat ggcggagaaa
    26281 aaacactttc acgtaattat ctgcgctgaa aagccgggaa acgtggactc tgccgatggg
    26341 ttttccgtaa ccgccaccca tgactatacc gcagtcatcc aggcggatat tgtgataatt
    26401 ccttactggg gaaccattac acaaaaaccg ccacaaaaac tgctggaagc cttaacgacc
    26461 gcacgggata acggcgcaca gattgtcggg ctttgcctgg gcacgtttgt gctcggctat
    26521 gcaggtttac tgaaaaataa gcgtgccgcc acgcactggg agttcgagcg tgaatttcag
    26581 gcacgttttc cacaaacaca tctggatatt aacgcgttgt acgtagacga tgacggcatt
    26641 attacctctg ccggtactgc cgcggcgctg gattgctgtt tgtatattgt tcggcaacat
    26701 tttggcagcg actatgctaa ccatattgcc cgacggatgg tcgtaccgcc atatcgcact
    26761 ggcggtcagg cgcagtttat tgagcaaccg gtgccgaaaa atacccatga tgaacgcatt
    26821 aatctcctgc tggattacct gaggcaaaac attgcgcaac agcatgatct cgactcgctg
    26881 gcgcagcgag taatgatgag tcgccgcaca ttaactcgcc attttatgaa agcgaccggt
    26941 tcgagtatcg ccgaatggct cattactgaa cgcttacgcc gtagccagga actgttggga
    27001 tccagtcagt tgcccgttga gcggatagcg gctgaggtgg gttttctctc acctgtgacc
    27061 tggcgtcagc attttaaatc tcacttcggc gtcagccccg ccgaatggcg caaaaccttt
    27121 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
    27181 gtaacgctat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
    27241 gtgaatcagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagt ggcgatggcg
    27301 gagctgaatt acattcccaa ccgcgtggca caacaactgg cggggaaaca gtcgttgctg
    27361 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
    27421 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
    27481 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
    27541 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cgctaatgtt
    27601 ccgacgttat ttcttgatgt ctctgaccag actcccatca acagtattat tttctcccat
    27661 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
    27721 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggcggg ctggcataaa
    27781 tatctcactc gcaatcaaat tcagccgata gcggaacggg agggcgactg gagtgccatg
    27841 tccggttttc aacaaaccat gcaaatgcta aatgagggca tcgttcccac tgcgatgctg
    27901 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
    27961 gttggtgcgg atatctcggt agtaggatac gacgataccg aagacagctc gtgttatatc
    28021 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
    28081 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agcttttgcc cgtctctctg
    28141 gtgaaaagaa aaaccaccct ggcacccaag acgcaaaccg cctctccccg cgcgttggcc
    28201 gattcattaa tgcagctggc acgacaagtt tcccgactgg aaagcgggca gtgagcgcaa
    28261 cgcaattaat gtgagtcagc tcactcatta ggcaccccag gctttacact ttatgcgccc
    28321 gactcgtatg ttgtgtgaaa ttgtgagcgg ataacaattt cacacaggat acagctatga
    28381 ctatgattac ggattcactg gccgtcgtat tacaacgtcg tgactgggaa aaccctggcg
    28441 ttacccaact gaatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    28501 aggcccgcac caatcgccct tcccaacagt tgcgcagcct gaatggtgag tggcaatttg
    28561 tctggttttc ggcaccagaa gcggttccgg aaagctggct ggagtgcgat cttcctgtcg
    28621 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgacgcg cccatctaca
    28681 ccaacgtgac ctatcccatt acggtcaatc cgccatttgt tcccacggag aatccgacgg
    28741 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa
    28801 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt
    28861 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag
    28921 aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg cagttacctg gaagatcagg
    28981 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcacaaa ccgaccacac
    29041 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gcggtactgg
    29101 aggcagaagt tcagatgtac ggcgagctgc gcgatgagct gcgggtgacg gtttctttgt
    29161 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg
    29221 agcgtggcgg ttatgccgat cgcgtcacac taggtctgaa cgtcgaaaac ccgaaactgt
    29281 ggagcgccga aatcccgaat atctatcgtg cggtggttga actgcacacc gccgacggca
    29341 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtacggatt gaaaatggcc
    29401 tgctgctgct gaacggcaag ccgttgctga ttcgcggcgt taaccgtcac gagcatcatc
    29461 ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc
    29521 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacaccc
    29581 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg
    29641 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc cgcgatgagc gaacgcgtaa
    29701 cgcgaatggt acagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg
    29761 agtcaggcca cggcgctaat cacgacgcac tctatcgctg gattaaatct gtcgatccat
    29821 cccgcccggt gcagtatgaa ggcggcggag ccgacacctc cgcaaccgat attatttgcc
    29881 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca
    29941 aaaaatggct ttcgttgcct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg
    30001 cgatgggtaa cagtcttggc ggcttcgcta aatactggca ggcgtttcgt cagtaccccc
    30061 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa
    30121 acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt
    30181 tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccggcgctg acggaagcaa
    30241 aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg
    30301 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggca
    30361 agccgctggc aagcggtgaa gtacctatga atgtcgctcc acaaggtaaa caggtaattg
    30421 aactgcctga actaccgcag ccggagagca ccgggcaact ctggctaacg gtacacgtag
    30481 tgcaaccgaa cgcgaccgca tggtcagaag ccggacacat cagcgcctgg cagcagtggc
    30541 gtctggcgga aaacctcagc gtgacactcc cctccgcgcc ccacgccatc ccgcaactga
    30601 ccaccagcga aacggatttt tgcatcgagc tggataataa gcgttggcaa tttaaccgcc
    30661 agtcaggctt tctttcacag atgtggattg gcgatgaaaa acaactgttg acgccgctgc
    30721 gcgatcagtt cacccgcgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca
    30781 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag
    30841 cgttgttgca gtgcacggca gatacacttg ccgacgcggt gctgattacg accgctcacg
    30901 cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggaa
    30961 gtggtcaagt ggcgattacc gttgatgttg aagtggcgag caatacgccg catccggcgc
    31021 ggattggcct tacctgccag ctggcgcagg ttgcagagcg ggtaaactgg ctcggattag
    31081 ggccgcaaga aaactatccc gaccgcctta ctgcggcctg ttttgaccgc tgggatctgc
    31141 cattgtcaga catgtatacc ccatacgtct tcccgagcga aaacggtctg cgctgcggga
    31201 cacgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc
    31261 gctacagcca acaacaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag
    31321 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc
    31381 cgtcagtgtc ggcggaattc cagcttagcg ccggtcgcta ccattaccag ttggtctggt
    31441 gccaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca
    31501 ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact
    31561 tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata
    31621 tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc
    31681 aaccgctgtt tggtctgctt tctgacaaac tcgggctgcg caaatacctg ctgtggatta
    31741 ttaccggcat gttagtgatg tttgcgccgt tctttatttt tatcttcggg ccactgttac
    31801 aatacaacat tttagtagga tcgattgttg gtggtattta tcttggcttt tgttttaacg
    31861 ccggtgcgcc cgcagtagag gcatttatcg agaaagtcag ccgtcgcagt aatttcgaat
    31921 ttggtcgcgc gcggatgttt ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca
    31981 tcatgttcac catcaataat cagttcgttt tctggctggg ttctggctgt gcactcatcc
    32041 tcgccatttt actctttttc gccaaaacgg atgcgccctc ttccgccacg gttgccaatg
    32101 cggtaggtgc caaccattcg gcatttagcc ttaaactggc gctggaactg ttcagacagc
    32161 caaaactgtg gtttttgtca ctgtatgtta ttggcgtttc ctgcacctac gatgtttttg
    32221 accaacagtt tgctaatttc tttacttctt tctttgccac cggtgaacag ggtacgcggg
    32281 tatttggcta cgtaacgaca atgggcgaat tacttaacgc ctcaattatg ttctttgcgc
    32341 cactgatcat taatcgcatc ggtgggaaaa acgccctgct gctggctggc actattatgt
    32401 ctgtacgtat tattggctca tcgttcgcca cctcagcgct ggaagtagtt attctgaaaa
    32461 cgctgcatat gtttgaagta ccgttcctgc tggtgggctg ctttaaatat attaccagcc
    32521 agtttgaagt gcgtttttca gcgacgattt atctggtctg tttctgcttc tttaagcaac
    32581 tggcgatgat ttttatgtct gtactggcgg gtaatatgta tgaaagcatc ggtttccagg
    32641 gcgcttatct ggtgctgggt ctggtggcgc tgggcttcac cttaatttcc gtgttcacgc
    32701 ttagcggccc cggcccgctt tctctactgc gtcgtcaggt gaatgaagtc gcttaagcaa
    32761 tcaatgtcgg atgcggcgca agcgccttat ccgaccaaca tatcaggacg gaatgatcgc
    32821 aatgaacatg tcgatgacag aaagaataaa agcaggcaag ctatttaccg atatgtgcga
    32881 aggcttacct gaaaaaagac ttcgtgggaa aacattaatg tatgagttta atcactcgca
    32941 tccatccgac gttgaaaaaa gagaaagcct gattaaagaa atgtttgcca cggtagggga
    33001 aaacgcctgg gtagagcccc ccgtctattt ctcttacggt tccaacatcc atataggccg
    33061 caatttttat gcaaatttca atttaaccat tgtcgatgac tacacggtaa cgatcggtga
    33121 taacgtactg attgcaccca acgttactct ttccgttacg ggacaccctg tacaccatga
    33181 attgagaaaa aacggcgaga tgtactcttt tccgataacg attggcaata atgtctggat
    33241 tgggagtcat gtggtcatta atccaggcgt caccatcggg gataattctg ttattggcgc
    33301 gggtagtgtc gtcataaaag acattccacc aaacgtcgtg gctgctggcg ttccttgccg
    33361 ggttattcgc gaaataaacg accgggataa gcaatattat ttcagagatt acaaagttga
    33421 atcgtcagtt taaattagca aaattgcctg atgcgcttcg cttatcaggc ctacaaattc
    33481 agtgttctac gttagccgca tccggcatga acaaagcgca gaaacaagcg tcgcatcatg
    33541 cctctttgac ccacagctgc ggaaaacgtg ctggtgcaaa acgcagggtt ataatcatca
    33601 gcccaacgac gcacagcgca tgaaacgccc agtccatcag gtaattgccg ctgatactac
    33661 gcagcacgcc agaaaaccac ggggcaagcc cggcgatgat aaaaccgatc ccctgcataa
    33721 acgccaccag cttgccagca atagccggtt gcacagagtg atcgagcgcc agcagcaaac
    33781 agagcggaaa tgcgccgccc agacctaaac cacacaccat cgcccacaat accggcaatt
    33841 gcagcggcag ccagataaag ccgcagaaac ccaccagttg taacgccagt gccaacagca
    33901 aaagtttgcg ccgatcctga tggcgagcca tagcaggcat cagcaaagct cctgcggctt
    33961 gcccaagcgt catcaatgcc agcaaggaac cgctgtactg cgcgctggca ccaatctcaa
    34021 tatagaaagc gggtaaccag gcaatcaggc tggcgtaacc accgttaatc agaccgaagt
    34081 ataggcccag cgtccaggcg cggggagtaa ataccacgcg aaccggagtg gttgttgtct
    34141 tgtgggaaga ggcgacctcg cgggtgcttt gccaccacca ggcaaagagc gcaacaacgg
    34201 caggcagcgc ccaccaggcg agtgtttgat accaggtttc gctatgttga actaaccagg
    34261 gtgtcagggc ggcaccaagt ccaccgccgc ccatcagagc cgcggaccac agccccatca
    34321 ccagtggcgt gcgctgctga aaccgccgtt taatcaccga aggcatcacc gcctgaatga
    34381 tgccgatccc caccccaccg agcagtgcgc tgctaagcag cagcgcactt tgggggtaaa
    34441 gctcacgcat caatgcaccg acggcaatca gtaacaaact gatggcgacg ctgcgacgtt
    34501 cgctgacatg ctgatgaagc cagcttccgg ccagcgccag cccgcccatg gtaaccaccg
    34561 gcagagcggt caacagggca gccacgctaa agctcattcc gcttgcctgg cgcaattgcg
    34621 gtagcagtgg accgacggag gtgagcagtg gtcgcatatt aagaccaatc agcaccagaa
    34681 ccagcagtat cgtgaacgtc ttttgatggt tgcgcatggc tgtcagaacg gtttggtcgg
    34741 cagatattta ccatctaagg tgatgaccgc acgttcgcca ccttccgggt ccgccacttt
    34801 cttaacgtcg agttttaagt taatcgcgct gataatgcca tcgccaaatt tctcatgaac
    34861 caacgctttc agggttgtgc cgtacacctg caacatttca tagaaacgat acatcgttgg
    34921 gtcggttgga atacggtcat caatgcagcc acgcagtggg atcatctgta acagtagaat
    34981 ggcgtcttcg tcgagatcca gcttcgcccc gacctggcgg gcggcgtcgg caggaagcgc
    35041 ctgctgaccc agcaaagccg cggtcacaaa ggcttctgcc agcccggtgc cgtcggcaat
    35101 ctcggcaaat gagagatctt ttttagcttt gctgagcaaa atggcatcgg caagatcaag
    35161 acgaatattg cggttaattt gcgactgaat catggtgcat ctcctgatgg tttaaaaata
    35221 aggttgcgct gcggtcggtt ggcgtagcgg tatggcacaa acgcgaggat tagcggccag
    35281 tggcacaaac tggcgggttg cgccgtcaaa cgctgcgatg ctgccgcttt caatgtcgta
    35341 gacccagccg tgcagggcga tccgcccctc ttcgagcgcc aggcgcaccg atggatgagt
    35401 ttgcaaatta gccaactgag caatgacgtt ttcacgtacc atcgccgccg cttttgacgg
    35461 taaatcggga tgcgggcgcg cctcattaac gacgcgggct gaatcggcat aacgcagcca
    35521 gtgggagacg gcaggcatat ggtccatgca ctgacagctg gcaatggcgg tcatcgcgcc
    35581 acagttggaa tgaccacaaa tcacaatgtc agatacccga agcgcagcga cggcatactc
    35641 caccgaagca gaaacgccac cgggttccgg cccgtaggaa gggacgatat tgcccgcgtt
    35701 gcgaataacg aacagatcgc caggctcacg ttgcgtcacc agctcaggga ccagacggct
    35761 gtcggagcag gagataaaaa gtgtgcgcgg gctttgctgt gtcgccaact gtttaaataa
    35821 ggcttcccgc ttcggaaatg cctcgcgctg gaatttaagg aatccatcaa taatctcttt
    35881 caccgttaac ctctgtctgt ctctggaatg agaggcagac tactcgccga ttgtcataag
    35941 gtaaaagtct catttatgat gagttccatt ggatttactt ataggttgcg aatgctctct
    36001 cgacatatca attattttct tgccgtggct gaacatggca gcttcacccg tgccgccagt
    36061 gcgttgcacg tctcccagcc tgcgctttcc cagcagattc gccagttaga ggagagttta
    36121 ggcgtgccgc tgtttgaccg tagcgggcga acgattcgtc tcactgatgc aggagaagtc
    36181 tggcgacagt acgccagccg ggcgttacag gaactggggg cgggtaaacg ggcgattcat
    36241 gatgttgccg atctgacgcg aggatcgctg cgtatcgccg tcacccccac ctttacgagc
    36301 tactttatcg gccccttaat ggcggatttc tatgcgcgct atcccggcat cacgctccag
    36361 ctacaggaaa tgtcgcagga gaaaatcgaa gatatgcttt gccgcgacga gttggacgtt
    36421 gggattgcct tcgcggctgt gcattcgccg gagctggagg caattccttt actgaaagaa
    36481 agtttagcgt tagtcgtggc gcaacatcat ccgctggctg cctgtgaaca ggtggcgttg
    36541 agtcgcttgc atgatgaaaa actggtcctg ctcagcgcgg aatttgccac cagagagcaa
    36601 attgaccact actgcgagaa agcggggctg catccacagg tggtcattga ggcgaactca
    36661 attagcgcgg ttctggagct gattcgccgc acttcccttt ccacattgtt accagcagcg
    36721 attgccacac aacatgacgg gcttaaagct atttctcttg ccccgccact actggagaga
    36781 acggcggttt tgttgcggcg gaaaaatagc tggcagacag ccgccgcgaa ggcatttttg
    36841 cacatggcgt tggataaatg cgcggttgtt ggcggaaatg aatcgcggta accactgaca
    36901 caattgcctg atgcgctacg cttatcaggc ctacgagttc agtgctctac gtaggccgga
    36961 taaggcgttc acgccgcatc cggcataaac tacgctcgct ttgtcaacac acagcccagc
    37021 cgatcaccgt ttgtaatcga tggcttctgg ctgctccaga tatacggtgg tttgtgccgg
    37081 ttgtgtactg gcaatcacct tgccgccacg taccgaataa cgtaccggaa cctgacggcg
    37141 cagcgcatca aatccatttt cagccggcag gataatcagg ttggcgctgt ttccggcggc
    37201 aatgccgtaa tcctgcaaat tcaacgtcct ggcgctgtgg tgggtgatta aattcaggcc
    37261 atcgttaatt tgccagtagc ccatcaactg acaaacatgc agccccatat gcagcacttg
    37321 cagcatattc gccgttccca gcggatacca cggatcgaag acatcatcgt gaccaaagca
    37381 gacgttaatg ccggactcca gcatctcttt aacgcgcgtg atgccgcgac gttttggata
    37441 cgtatcgaaa cgcccttgca gatgaatatt gaccaacggg ttggcgacaa agttaatacc
    37501 ggacattttc agcaagcgga acaggcgtga ggtatacgcc ccgttatagg agtgcattgc
    37561 cgtggtgtgg ctggcggtaa ctcgcgcgcc catgccttca cggtgcgcca gggcagcaac
    37621 ggtttcgaca aagcgcgact gttcgtcatc gatctcatca cagtgaacgt cgatcagacg
    37681 gtcgtatttt tgcgccaggg cgaaggtttt atgcagcgac tccacgccgt attcacgggt
    37741 aaattcaaaa tgcggaatcg cccccactac gtctgcccct aagcgtaacg cctcttccag
    37801 caacgcttca ccgttgggat acgacaaaat cccttcctga gggaaggcga cgatttgcag
    37861 atcaatccac ggcgcgactt cctgcttcac ttccagcatt gctttcagcg cagttagcgt
    37921 tgcatccgaa acatcgacat gggtacgcac atgctgaatg ccgttggcaa tctgccattt
    37981 cagcgtttgc catgcgcgtt gtttcacatc gtcatgggtt aataacgctt tgcgctcggc
    38041 ccagcgttca atgccttcaa acagcgtgcc ggactgattc cagttcggtt gtccggcggt
    38101 ttgcgtggtg tccaggtgaa tatgtggctc cacaaacggc ggtataacta aaccttgttc
    38161 ggcatccagg ctgttttcag ttatgggcat cacgccggat tgcgcatcaa tggcgctgat
    38221 ttttccgtcc tgcagatgaa tctgccacag cccctctttg cctggtaacc gggcgttaat
    38281 aattgtttgt aaagcgttat tcgacactgt tagcctccac atgcgtcatt gctgctgtcg
    38341 ttttacgatt caaaatcggg ttaaggatca gatagctcag cgcgccacct aataccgcgt
    38401 tgaccggaac aattcccggt aaccagtggc ctgcggcaat ccccaaggcg accgccagaa
    38461 tcgccaccca attgacactc atcatacgcg tggtcgcaaa gtgctcatag cggcgacggt
    38521 tcatcagata gtcggcaatg atcacgccac ccaccggagg aatagctgcc gaaaggaagg
    38581 tcaaccagcc gacaaaattg ttatacagcc ataatgcgca gaccgtaccg ataataccgt
    38641 taatcaccga aagggttttg ctcgacatcc cggtgatgtt ggcgaaacct aaacccgacg
    38701 catagagcgc gttatcgttg gtggtccaga tattcagccc gagcaccaca atcgcaggca
    38761 gcagcaggcc ctgagcaatc atcacatcag agatatccgc catccccagt gccgcagcgc
    38821 ccgctgcacc gaaaataaac atcaacgagt tgccgagaaa aaaggccacc atcgccacca
    38881 gcacagccag tttggcattg cgaccaaagc ggacaaagtc agcggtgagc gtgcccgcac
    38941 tgataaatga ccccacaacc agcgccagcg cgacattgaa atctaacggt tgtgcgggaa
    39001 cgaccgcttt taatgcgtcc aggccgccca tgccgttaac agccagccac acggaatagc
    39061 cgccaaggca ggcgatagcc ggaaccgcaa tcaccgaaag aaccgtcagc gccgaaatgc
    39121 caaagaagac ggtgacggtc atcagtaaac cggaaacggc aatcagcaaa ttaatatcca
    39181 gcccagttgc cttgcccacc ggaatagcaa acatcgccac gccaacgcca aaccagccaa
    39241 cctgggtgcc gcccagcagc agtgaaggca gccatgagcc tttaacacca aaagagaagc
    39301 gagcaagaag atgggtggtc aggccggttt ttgcgccaat gtaaccaaga aatgaagtgt
    39361 aaataccgag gagaagatta ccgatgagaa ctgcgaggaa gaaatcatga tagctaagac
    39421 cggttccgag agtgccgccg gtccacatac tggcggaaaa gaaggttaat cccagcatga
    39481 cgaacgtcaa tgccaatacc cctttccgcg ccgactgcgg gactggcccc tggctaaagt
    39541 tgttatcttg cgacacgaaa ttcccccttt ttgctgtttg aaacccaaaa aatccgccgc
    39601 attctattca tctgaagata aaaagcaatc gttttcgtgg ggaaatatat tttttatatg
    39661 aatgagtgca aaaaggtggg ggaaaattat cggtgtaaaa acagtgggca ggcatgataa
    39721 gacgcggtaa gcgtcgcatc aggcatcggc gcatggtgtc ggatgcggcg cgagcgcctt
    39781 atccgaccta cggttcaggc ctgtaggcat gataagacgc gtcagcgtcg catcaggcat
    39841 cggcgcatgg tgtcgaatgc ggcgcgagcg ccttatccga cctacggttc aggcctgtag
    39901 gcatgataag acgcgcctgc gtcgcatcag gcatcggcac acggtgtcgg atgcggcgcg
    39961 agcgccttat ccgacctacg gttcaggcct gtaggcatga taagacgcgt cagcgtcgca
    40021 tcaggcatcg gcgcacggtg tcggatgcgc cgcgagcgcc ttatccgacc tacggttcag
    40081 gcctgtaggt atgataagac gcgcctgcgt cgcatcaggc atctgcgcac ggtgtcggat
    40141 gcggcgcgag cgccttatcc gacctacggt tcaggcctgt aggcatgata agacgcgcca
    40201 gcgtcgcatc aggcatcggc gcatggtgtc ggatgcgcca cgcgacgcat ccgacaatac
    40261 cttactcttc catcgcctgg cggatctgat ccaacgacgc aggatcatca atggtcgtca
    40321 gatccccagg atcgcgtcct tcgcaaatcg cctggatcgt gcggcgcagc atttttccgg
    40381 atcgcgtttt tggcaattgc gagacaaacc agacgtgcgc cgggcggcca aagttgccaa
    40441 tctggctgtc caccagcgcc ataatcgcct tctcttgcga atgcgccaca tcacgatctt
    40501 ccagactgtc gctctctttc ggaatgacaa acgccaccgc tacctgccct ttcagcgcat
    40561 ctttcacccc aaccaccgcc acttcggcaa cgcccggatg actggagata ctctcttcaa
    40621 tctcgcgcgt ccccagccga tgcccggcaa cgttaatcac atcgtcagtg cgcccgagaa
    40681 taaagtgata accgtcagca tcgcggatgc cccagtcaaa ggtggcgtac accgggcggg
    40741 aaaacagcga ccagtacgtc ttcacaaagc ggtcgtcgtc gccccagatg gtctgaatac
    40801 accccggcgg cagcggccct tccaccacca gcatcccttt ctcgttgacg ccacacggtt
    40861 cgccggtaac ttcattaagc aactgcacgt tatagccata catcggcacg ccggggcttc
    40921 ccagacgcgt cggcctgtcg tccagaccgc gagcaatcgc cataatcggc cagccggatt
    40981 cggtctgcca gtagttgtcg atgaccggca catccagcgt attgctcacc caactggcgg
    41041 tcggctcgtc cagcggttct ccagccagat agagcacttc cagcgacgag agatcgtgtt
    41101 tgcgaatttc agcggtaggg aattttttca gcacgcgaat ggcggtcggc gctgagaaca
    41161 tccggctcac ctgatatttc tcgacgattt tccaccacac accgcagtcc ggccaggtcg
    41221 gtaatccttc gtaaacgata gtcgccatcc ccgccagcag cggcgcgtag acgatatacg
    41281 aatgccccac cacccagccg atatccgaag cacaaaagaa cacaccgccc gctttgccgc
    41341 caaaaatggt gtccatcgag gtcgccagcg ccaccgcata tccgccgacg tcacgctgca
    41401 cgcctttagg tttgccggtc gtgccggaag tgtagagaat gcaggaggtt tcgttggatt
    41461 ccagccacgc caccggcacc cgcgcgccga tgtgttgatg gtgtaacaac gcgaaatcga
    41521 catcccgccc gctaacgcgc gccattttcg ccagcccgcg atccaccagc aaaacatggc
    41581 gcggctggtg ctgcgcctga cttatcgcat cgtcgagcaa ttttttatag ggaatgattt
    41641 tgccaccgcg cgctccggca tcagccgaga caatcagcac cggtttagca tcatcaattc
    41701 gcgccgccac gctatgagag gcaaatccac caaacaccac cgagtgaata gcgccaatgc
    41761 gcgcgcaggc cagcagggta atatgcgctt cggcaatcat cggcatatac accagcaccc
    41821 gatcgccacg ctgcacgccc agcgagcgca gcattgacgc caccgcgttt acttcgtcat
    41881 gaagctggcg aaaggtaaag gtgcgctctt cttctgtttc cgaagagacg gcaatcagcg
    41941 ccagcgcctc tggctgtttc tccagccagc ggtcgatagc gttgtgacac aagttggttc
    42001 ggccttcaca aaaccaacgg gcaaacggca gattgctgtg atcgagcgtt tgcgtaaagg
    42061 gcgtctgcca gtcaatacgc cgggcctgct cggcccagaa ctgctccggt tcgttaatcg
    42121 aacgctgata aaattcgcta aaagacataa tgctctcctg ttgaacttac gccttaccgc
    42181 cgtttactta aatgacgtac aggtcgagat actcattgac cggcatctgt tccaggcgag
    42241 ctctgtcgag agaaacctcc agaatgcgct gctgctggcg agtcgggaac tggcgcgcga
    42301 gattgatttt gaatttatct accagcttcg gaatgccatc ctgacggcgg cgagcatgac
    42361 caatggggta ctccaccacc acttcttcaa agcgcgtacc gtcagtgaac tcaagggtaa
    42421 tggcattggc gatggcgcgt ttttccgggt cgtggtagtc agcggtaaac gccggatctt
    42481 caaagcaatt gatcttctcg cgcagggcgt caatgcgttt atcttgcgca acgttgtcct
    42541 cgtaatctgc cgccgttaag cgcccgaaca gcagcgggat cgccaccatg tattgaatgc
    42601 agtggtcgcg gtcagccggg ttattgagcg gccctttttt gtcgatgatg cgaatacagg
    42661 cttcgtgggt gcgaatggtc actttttcga tatctgccgc cgttttgcct gctgcctgca
    42721 tctgttcata gagcgtcatc gccgcttcaa ctgccgtctg ggagtggaac tccgccggga
    42781 aggagatttt gaacagcaca ttttccataa cgtaggaacc gtacggacgc tggaagcgga
    42841 acgattcacc tttaaaggag acgtcgtaga agccccatac cggcgcggtc agggctgacg
    42901 ggtaacccat ttcgccggtt ttcgccatca gcgccagacg taccgcgcgg gaagtggcat
    42961 cgcccgccgc ccaggattta cgcgtgccgg tgttcggcgc atggcgatag gtgcgcagcg
    43021 actgaccgtc cacccacgcc agcgaaacag cgttgagaat ttcctcgcgg gtcaggccga
    43081 gcatttcggc gaccacggcg gtggaagcca ctttcactaa cagaacgtgg tcgaggccga
    43141 cgcggttaaa ggagttttcc agcgcgatgc agccctgaat ttcatgggct ttaatcattc
    43201 cggtcagcac ctgtttcatg gtcaacggcg ctttgccgct ggcgaccgcg ttgcgcgaaa
    43261 gccagtccgc cgttgccaga atgccgccga gattgtcgga aggatggccc cactctgccg
    43321 caagccaggt atcgttgaaa tcgagccagc ggatcatcgc gccgatgtta aatgccgcct
    43381 ggacggggtc gagctggaac tgagttccgg ggacgcgcac gccgttgggt acgacggtgc
    43441 ccggaacaat tggccccagc agttttttac aggccgggta ttcgagagct tccagaccgc
    43501 agccgagcgt gtcgagcagg cagtaatgtg cggtgtcgta ggccacttta gagctgattt
    43561 cgtagttcat gacgtaatcg acgatatcaa cgatttcacg atcaaattcc gggcggatgt
    43621 tgttgatttg agctgacatt gggtacgttt ccttattgtt attcgtagag gtttactggc
    43681 gcttatccag cgcgacaaac tggcggtctt ccggtccaac ataattggcg gaaggacgga
    43741 taattttgtt gtcctgacgt tgttcgataa tgtgcgccgc ccagccggtg acgcgggcga
    43801 taacaaacag tggtgtgaac atctcggtgg gaacgcccat catgttgtag gaaacagcgg
    43861 agaaccagtc gagattgggg aacatctttt tgctctccca catcaccgtt tccagacgat
    43921 cggcgatgtt gtacatcttc agcgagccgc cttcctgcga gagctgcttc gccacacgtt
    43981 tgatcacctg atgacgcggg tcggcgatgg tataaaccgg atgaccaaaa ccaatgacca
    44041 cttctttgtt ttccacccgc ttgcggatat cggcttcggc ttcgtctggc gtttcgtagc
    44101 gttgctggat ctccagcgac acttcattcg ccccgccgtg ttttggcccg cgtagtgcgc
    44161 caatcgcgcc aataatcgcg gaatacatat cagagccggt gcccgcaatc acccgactgg
    44221 taaaggtgga ggcgttaaac tcgtgttcgg cgtacagcac cagcgaaata tgcatcgcct
    44281 tttcccagct ttgcgatggc ttttcgccat gcagcagatg caggaagtga ccgccgatgg
    44341 agtcgtcatc ggtttccggc tggatacgtt cgccgttatg gctgtagtga taccaataaa
    44401 ggagaatcga gctaagtgat gccagcagtt tgtcggcaat atcccgcgcg ccagagacgg
    44461 tatgcccctc tttttctggc agcgtgcagc cgagcgcgga aacgccggtg cgcataacat
    44521 ccatcgggtg tgacgccgcc ggtaaggctt ccagcacggt acgcacgtta gccggtaaac
    44581 cgcgcagggc tttcagtttc gttttgtagg cggcgagttc gtcacgggtc ggcagtttgc
    44641 cgtggatcag cagatgcgcc acttcttcaa attcgcaatg ttccgccaga tcaagaatat
    44701 cgtagccacg gtaatgcaag tcattgccac ttttacccac ggtgcagagc gccgtattgc
    44761 ccgccggaac gccagaaagc gccacagatt ttttcggttt aatgacatgg gtactgtttt
    44821 gcaggatcgt tgtgtcgctc atattgtcct cgtcattgct atcatttgta gggtcgagat
    44881 tgttgtcgga tgcgacgcca tgcgtcttat ccgacctaca gttcagtaca ccccgtaggc
    44941 ctgataagtc gcgtcagcgg cgcatcaggc atcggttgtc ggatgcgacg ccatgcgtct
    45001 tatccgacct acagttcagt tcaccccgta ggcctgataa gtcgcgtcag cgacgcatca
    45061 ggcatcggtt gtcggatgcg acgccatgcg tcttatccga cctacagttc agtacacccg
    45121 taggcctgat aagccgcgtc agcggcgcat caggcatcgg ttgtcgaata caaccaacgg
    45181 gcgtttttta tttcacctgg ctacgggcaa acaggtcgtc gagcttctct tcgtactggt
    45241 agtagttgat gctttcgtac agctcgttgc gggtttgcat ggtgtcgatg acgcttttct
    45301 gcgtgccttc ctgacgcagg acgttgtaga catgttcagc ggcgcggttc atggcgcgga
    45361 acgctgaaag cgggtacagc gccattgcga catgggcgct gcgtaattcg tcggtggtaa
    45421 acagcggcgt ggcaccaaat tcggtaatat tggcgaggat cggcacctgc accgcatcgg
    45481 caaactggcg gtacatggcg agttcggtaa tcgcctccgg gaacaacatc tcggcacccg
    45541 cttcaacata ggcctgcgca cgctcgatcg ccgcatccag cccctctacc gccagcgcat
    45601 cggtgcgcgc catgatcaca aaatcaggat cggttttcgc atccaccgcc gcgcggatcc
    45661 gatccaccat ctcctctttc gagacgatcg ctttattcgg acgatgaccg cagcgtttcg
    45721 caccaacctg atcttcaata tgcaatcctg ccgcaccagc tttaatcatc gatttcacgg
    45781 tgcgcgccac gttaaaggcc gaagaaccaa aaccgatatc cgcatccacc agcagcggca
    45841 gcgaacaaac gtcggtgata cggcgaatgt cggtcagcac atcatcaagg gtagaaatac
    45901 cgagatcggg cagccccagc gaccctgccg ccacgccacc gccggagaga taaattgcct
    45961 gatatccggc acgctgcgcc aacagcgcat ggttggcgtt aatggtgcca acaatctgca
    46021 atggattttc tttagtcagt gcagcgcgaa acactttacc tggggagtgt agagacatag
    46081 cccatccttt gttatcaact tgttatttgc gttgataaac acaaagcaag gggtgtgcca
    46141 caattgcctt aataaaatta aatctttaat ttcaatttgt tagagataaa gtcatgaact
    46201 aatccgcaaa tatgcgtttc agttaacgtt tcaggcaatg tttcaccgcg tttcattgca
    46261 acaattatga aacaagacta aacccaatat tcggtttctt aactttgcgg tgcgctatgg
    46321 cacatccacc acggctgaat gacgacaaac cggttatctg gacggtatct gtaacgcgcc
    46381 tgttcgagct gtttcgcgat atcagcctcg agtttgatca cctggcgaac attaccccta
    46441 ttcagcttgg ctttgaaaaa gcagtgacct acatccgcaa gaaactggca aacgaacgct
    46501 gtgacgccat catcgctgct ggctctaacg gcgcgtacct gaaaagccgc ctgtcagtgc
    46561 cagttatttt gattaaaccg agcggctacg atgtgttaca ggcactggca aaagccggaa
    46621 agctcacctc ttctatcggc gttgtcactt atcaggaaac tattccggca ctggtggcgt
    46681 ttcaaaaaac ctttaatttg cgcctcgatc aacgtagcta cattaccgag gaagacgcac
    46741 gcgggcagat taacgagcta aaagctaacg gcaccgaagc ggtggtcggt gcggggctga
    46801 ttaccgatct ggcagaagaa gccggaatga ccggaatttt tatctattcc gccgccaccg
    46861 tgcgccaggc gttcagcgat gcgctggata tgacgcgcat gtcgttacgc cataacactc
    46921 acgatgccac ccgcaacgcc ctgagaactc gttacgtgct gggcgatatg ctcggtcaat
    46981 caccacagat ggaacaagta cgacagacta ttttgctgta tgcccgctcc agtgcagcgg
    47041 tgttgattga gggggaaacg gggacgggca aagagctggc ggcccaggca attcatcggg
    47101 aatattttgc ccgccacgat gcgcgacagg gcaaaaagtc gcatccgttt gttgccgtca
    47161 actgcggggc gattgccgaa tcgctgctgg aagcggagct gtttggctat gaggaagggg
    47221 cgtttaccgg ctcgcgacgc ggaggtcgcg ccggactgtt cgaaattgcc cacggcggca
    47281 cgctgttttt ggatgagatt ggcgaaatgc cgctaccttt gcagactcgc ctgttacggg
    47341 tgctggaaga aaaagaggtc acccgcgtcg gcgggcatca gcctgttccg gtagatgtac
    47401 gggtcattag cgccactcac tgcaatctgg aagaagatat gcggcaaggg cagtttcgcc
    47461 gtgatctgtt ttatcggctg agtattttgc gtctgcaatt gccaccactg cgcgagcggg
    47521 tggcagatat tcttccgctg gcggaaagct ttttgaaagt gtctctggcg gcgctctccg
    47581 ccccattttc tgccgcatta cgccagggat tacaggcaag cgaaaccgtg ctggtgcact
    47641 acgactggcc aggtaatatt cgtgaactgc gcaatatgat ggagcgactg gcgctatttt
    47701 taagtgtgga accgacgccg gatttaacgc cgcagttttt gcagctgcta ctgccggaac
    47761 tggcacgcga gtcggcgaaa attcccgctc cacgcttact gacaccacaa caggcactgg
    47821 agaaatttaa aggcgataaa acagcagcgg cgaattattt aggcatcagc cggacgacgt
    47881 tctggcggcg gctgaaaaac tgaactgccg aatgcagcgc gaacgcttta tccggcataa
    47941 atttcaatag atgatgatca ccgataaact tacgcatcgg ggatgtttac atagggttca
    48001 gaattacttc ttcttataaa tatttgccgt gccgtggatc ttattgtcag tttgaccgga
    48061 ggtcagtacc aacacatcag cccctttttc atccgctttt ttgatcaaat cctctttcgc
    48121 atctgcggtc gacatttcat tactggttga aatatcacct attttttcat attgcgattc
    48181 gactttttca aattccgctt tagtcatcaa ttcagcggca tagacattgg taacggcaaa
    48241 cgctaacgca ccgactaaca ttttagagat tattttcata ttacggctcc agagttgtta
    48301 tcacactatc acccacccac attattgggt tgattaataa ttagttcagt cagggcataa
    48361 acgccagtaa ccggaaataa gacactttgt gatctctctc gcacccgatt atttttggta
    48421 gaaatatgat gaagttactt cacttattta tggatgaaat cactatggat cctttgcacg
    48481 ccgtttacct gaccgtagga ctgttcgtga ttactttttt taatccggga gccaatctct
    48541 ttgtggtagt acaaaccagc ctggcttccg gtcgacgcgc aggagtgctg accggactgg
    48601 gcgtggcgct gggcgatgca ttttattccg ggttgggttt gtttggtctt gcaacgctaa
    48661 ttacgcagtg tgaggagatt ttttcgctta tcagaatcgt cggcggcgct tatctcttat
    48721 ggtttgcgtg gtgcagcatg cgccgccagt caacaccgca aatgagcaca ctacaacaac
    48781 cgattagcgc cccctggtat gtcttttttc gccgcggatt aattaccgat ctctctaacc
    48841 cgcaaaccgt tttatttttt attagtattt tctcagtaac attaaatgcc gaaacaccaa
    48901 catgggcacg tttaatggcc tgggcgggga ttgtgctcgc atcagttatc tggcgagtct
    48961 ttcttagtca ggcgttttct ttgcccgctg tgcgtcgtgc ttatgggcgt atgcaacgcg
    49021 ttgccagtcg ggttattggt gcaattattg gtgtattcgc gctacgcctg atttacgaag
    49081 gtgtgacgca gcggtgagca gataatttac tctctacgta atcaacctga tttgccgaat
    49141 cccgaccatc ggcgatgata gcccccgtaa tgcgtggtcc ttatttccga taagtttact
    49201 tgagtcttta gcttcccatt tcagcccact ctcccctgtg tgggcttttt tcattggtct
    49261 gacccgtcct gaatagccaa acaatggcaa tgttccactt tgcagggatg ataataaggg
    49321 gatctgattc agaagagttt tgaaaagttg gaccgccatc gaggcctcga accccgtacc
    49381 atacaacaat cctggttgtc gtctgctctt cctgatgagc taatggcggt ttttgatatg
    49441 agccttacag catattttct gtcaggcttg agtgcgatgc gtaaggtaca gaaaagaccc
    49501 cacacaaaca tgatggaatt atgatttttt tcatatgaat gtcaacccac acattcacaa
    49561 aacatctgca tgagatcatt ttttatacac cgcatggctg cgaaaccact actggtgctt
    49621 ttttaagcga agcgtcgccg atcacaacaa aatatcggcg aaaaaggcgg tggaatgcca
    49681 gttggcatac agatatacag atgaaatgat tacctgcatt gcgattcact ctctggtgga
    49741 ataacaactt gcaggtggaa ccctttctga actaaagagt gtagcggatt ttaacgtgct
    49801 tatttacagg ataatatctg tcacctaatt cctgcactct attagatatc cattcattta
    49861 atcgtgcatc atctctgtat gtttttacgt ccagaacaga gagcaataac atcaccagtg
    49921 attcaggaaa aacagggtag atatttttta agtgtaataa taatccatgc gcaagtgcct
    49981 gcttaatggg cgcgtctgtc tccggaatag acgacatttc gcgaagccat ttttccacat
    50041 gcatacactc tttcccttta gggaaaattc tcagcaaagg catatggtgt tcaagaagaa
    50101 tacatttgca atctgttcgc actgaatcga taaactgcac taatttctta agcccggtaa
    50161 agcgtccagc ctgcacgttt gctttaatat caagaaacgc atcatcctgg tcatataccc
    50221 aaactgtccc tttttgattt tcaagtatgc gaaaaacaaa cttaaccggg tgatcagggt
    50281 aatccatcca gtcataaaat ccagggaaat cattaaactg cacatttatg gtgagtgcgt
    50341 tatcgtctga ccactcacag tctcgtacgg cagccatgag atgaatgagt ttgtcgtcat
    50401 cgactaaatc atcacgacaa tcataaggat aatcataata ttgtttaatc aaaaccttga
    50461 atatatcgac acaggaaacc agggagcttt tgatatcctc tggtaactga ttgtttttaa
    50521 cagtattaag tatgctttgt tgcgtatatg gcatcaaatt attgtgcgga gcttttaatg
    50581 atatcatcac gatctctctt aaataaaaat aaacgtcgca gataataaag ctctgattaa
    50641 accaggcact atcagaaatc gctcataact aacgtgtgaa gtattgtgta ctggagggag
    50701 ttaatttaac aatttgaatt tacgttatag gttttactgc tactttgata ttatttcgct
    50761 ctccttaact aaaaagttaa gtggagagcg aaattaatat gttaaaccac aggatattta
    50821 ttaatttttt cagtctgtta gtgtgcgatt atcgataaca aaacgatatt tcacatcacc
    50881 acgcagcatt cgctcatagg cttcattaat ttgatcggcc cgaatcatct ctatatcagc
    50941 cacgatgcca tgttcggcgc aaaaatcgag catctcctga gtttctggaa tgccgccaat
    51001 catagaaccg gctatcgcac ggcgtttcat gatcaggttg aaaacttccg gcgatttatg
    51061 cggtgtcgca ggcgcaccaa ccagcgtcat ggtgccatca cgcttcagca aggtggtaaa
    51121 atcgtcgaga ttatgtggcg cagctactgt attcaaaatg aaatcgaaac tcttcagatg
    51181 agccgccatc tcatcggcat tgcgtgagtt aacaacttca tcggccccca gggcttttgc
    51241 cgcttcgcgt tttgcctcag aagtggtaaa tgccaccaca tgtgccccca tcgcgtgggc
    51301 cagcttaatc cccatatgtc ccagaccgcc gatgccgacc acgcccactt ttttacccgg
    51361 cccggcctgc cagtgacgta gcggcgagta cgtggtgatc cctgcacaca acaaaggagc
    51421 caccgccgcc agctgctctt gcgggtgacg aatacgcaga acatatcgct catgaacgac
    51481 gatctgttgt gagtagccgc ccagagtatg gcccggttcg tccggcgttg gcgagttata
    51541 ggtgccggtc atgtgatcac agtagttttc cagcccgtct tcacactctt cgcaatgttt
    51601 acaactgtcg acaatgcagc cgacaccgac cagatcgccc agcgcatgtt tttctacctg
    51661 atcaccaacg gctaccacac gccccacaat ttcatgaccc ggcacgcagg gataaaccgt
    51721 ccccgcccac tcggaacgga cctggtggag atcggaatgg cagacgccac agtaagcgat
    51781 ttcgattttg acatcattcg gtcccggttc acgccgtgtg atatccatcg gttcaagtgg
    51841 ttgtttagcg gaatatgcac caacagcttt gatcttcatt gtgtttactc ctgattagct
    51901 atgtgtattt ggcgatacct gatatgtcat cgcggcaaaa cgcgtccatt gaatatagcc
    51961 aatatttaaa ccacccgatc cagccccaac atgccgcgag cctgaatgaa acagagcagc
    52021 aacgttcccc ataaggcgag cgcctgtaag tgtgatattt gctatccgta aaagactaaa
    52081 atcggcaaaa ctgatgatct tttcacgtag acaaatagtt atctacacaa cgtcaataag
    52141 ggcatattcc taaaagataa tgtccatttc tgccgacaca ctcatattca gattgcccca
    52201 ctccctgggg cttaaatcta ttactattca ccgttgcagg cgtttcaaat gcacatcaac
    52261 ctgccacact cccccacacc agttgccctt tatggaacgt tgcggttctc ggcgagatac
    52321 gcgccaccgc ctcggcagaa caggaggcgt ccaccagcac aaagctggcg tcatcctgcg
    52381 cttttggcca tacacgctcg cctttttcat tcagcggcaa tacatcgcca gtggctagaa
    52441 acagcgaacg ggagaggttc tgttcgttag gacgaatata gagctgcgcg tagagattgg
    52501 ctttttccag catgtcgccc agaccataag gcgaccagtg gtcgataacg ctgtcagtgc
    52561 cagtcatcac ttttacgcct ttgtcgtgca actgtttgag cggcatatgc agcgtgccaa
    52621 tcggcaccgt cgaggcgata gaaatttgct gcgccgccat ccgattcgcc agcgcatcga
    52681 cctgttgttc gttgagcgta gccagcgcaa aggcgtggct gatggtcagc ttacctttca
    52741 gttgcggcgt tttctctacc gtttcaacca tataattgat ggctgccacg cccgacggag
    52801 tggtttcgtg caggtgaata tcgacgcctt tgtcgtagtc cagcgcaatc tggaacatgg
    52861 tatcgaggga tttttccatc gcgccatcga cactggtcgg gtccagcccg ccgacgtaat
    52921 gcgcacccgc ctgcattgct tcacgcatta agggttccga tttcgacagc agcaaaccgt
    52981 gctgcgggaa ggcgacgatt tcacactcaa agcccgcctg acgtcgcgcc agcaccgcct
    53041 gcaaattttg cagatttttc aggccggaaa ctggttcgat attgcagtga ctgcgggcaa
    53101 tggtggtgcc tttcgactgc aatagatcga tcagtttttc cgcccgctcc tgcgtgtacg
    53161 gttgcagctc cggcagcatt ttctgctcga gtttgatcat gtcctggatg gtggtgcctg
    53221 ccggacgatt gagcgagcgc cacggcccgc cgtagaaggt tttgtcgaga tgaatatgca
    53281 tgtcgcgggt ggtgggcagc atcagcttat cgccagcgtc atagtgcggc agcgtggcat
    53341 ctgggtgctg cttattctcg cgcagcgcga caattttgcc gttctgaatc tccacggtct
    53401 ggcgcgccgt gcgggtctgc accgccacgc cattttcgta gtcaaaaccg gtttccagca
    53461 gcacgttatc gagatagtaa tgcgggtcag tgattttcat ggtgttgttc gcttcgcagt
    53521 ttagggtgcc agctaccgcc gcatgggcga gcggcacaga ggtaccaaac agcgcggcgg
    53581 cggtgaccat cttaccgctc tggctcagaa attcacggcg gctattgctt tctttcatct
    53641 tacgtcctta cgttttaata atatgggtgc ccgtttcacc gcgcagcgcc gcaggcaggc
    53701 attccggcgt ggtgataatc acttctttgc cgccctgttc caggaacgtc aggctggcga
    53761 tgatttttgg caacatgctg ccgggcggga aatgaccttc ctgcatatag cgagtcatgg
    53821 tggcaatatc cacccgatcg agcgcctgct gctgcggttt gccaaagtga atacacactt
    53881 tttcgacgcc agtggtgatc accagaatgt cggcgtgaat ttcacgggcc agcagcgcag
    53941 tagagagatc tttgtcgata accgcgtcca cgctttggta atctcccgcg tcagtacgca
    54001 ctaccggaat tccaccgccg cccgcgccaa ttacgacaaa accttgttgg atcagcgctt
    54061 taatggcagg tgcttcgaca atacgtttcg gttccggcga ggcgaccacg cggcgatagc
    54121 cccgcccggc atcttcaaca aaacaccagt cagggtttgc cttttgtaat tcgtcacgct
    54181 ggctgtcact aaagaatgcg ccgatgggct tggtgggatg ggcaaaacct ggatcgtttt
    54241 tatccacttc cacctgagtc accacggtga cggctttttt ctcgccgtga cgcgccagtc
    54301 ggttgttcag cgcctgttgg atcagatagc caatgccgcc ttgcgtatcc gctacacagt
    54361 tcgccagcgg cgttaatggc agcccttcgc gctcgtgggc aatctccgca cggcgtaaat
    54421 ccagcccgac ctgcggcccg ttgccgtggg tcagcacaat gtcgtaatcg gaagccagca
    54481 tttccagcac cgtatcggcg acggctttca ccgcctccgc ctgatgctca atcgactggc
    54541 tggcgttatc tttgataatg ctgttgccac caatggcaac gaccacaagc tctttcatgt
    54601 tgttatgtcc gctacagagg gtgatgaggt tatgaccaac gccagactgc ctgatgcgct
    54661 acgcttatca ggcctacaat tctcgcgcca ctcgtaggcc ggataaggcg tccacgccgc
    54721 atccggcaat ggtgacaaat gcctgatgcg ctacgcttat caggcctaca aattctcgtg
    54781 ccattcgtgg gccggataag gcgttcacgc cgcatccggc aatggtgaca aatgcctgat
    54841 gcgctacgct tatcaggcct acagattctt gcgccattcg taggccggat aaggcattca
    54901 cgccgcatcc ggcaatggtg acaaatgcct gatgcgctac gcttatcagg cctacaaatt
    54961 cttgcgccac ttgtaggccg gataaggcgt tcacgccgca tccggcatga acatgtaggt
    55021 cagacgccgt aacgttcgca ccagccgaga atggctttct cgaagcaggc gaatggtgga
    55081 tgcacaatgc ccgcgccaat catgccgacg cccgcatctt tatgggcgat ggcggtgttg
    55141 atcactggca gaatgccgct gctacccacg cgggtgatgt cgattgccga cggcacgccc
    55201 ataaaaccga gcagcggaat ggtgacgttg gggttttcgc caagggtgat ttcgcgcatc
    55261 tgacgggaga aatcgatagc ctcttccacc gtgccaccca ccagcgcgac gattgcaggt
    55321 gcggtcgcca tggcaaaccc gccaataccg taggtttctg tgatggcgct gtcgccgata
    55381 tccagccccg aatcttccgg cttataacca gcaaacatcg ggccgatcac ctgctgcgct
    55441 gggccggtaa accaatgccc cggcaggccg ctgacccgca ggccgaactc gacgccgtta
    55501 cgcgccatgg tggtaacgac ggtgctgtat tcgatgccgt gcgccgcatc catcgccgct
    55561 ttacacatcg ccatccacgt cgggccggag aagtagtcgc tgctggcgac aaactcaaac
    55621 acttcgcgct gttgctcgac ggagtagccc gcctgaataa tccccggcgt cagcgcctga
    55681 atcagcagcg tcgtcccggc gttattgcgg ttatggcact catcgcccat atgcagcgcc
    55741 tgcgccagca ttaagcgcag atcgatctcg ccgataattt tcatcgcgtc gcgcagtatt
    55801 ggtccctgca catcacgcat ccagttcagg cggtcaatca cgctctggtc gttagcgccc
    55861 atacgcaaaa tcttcgccat ctgctcgctc atgttggtat aagcgatgtt gccgtaggtt
    55921 ttgtttttta cgatgtgcat aaacatcgag gccgaggtaa cacccgccat cgatcccacg
    55981 cagtcgtgct cgtgacacgg cgagaaggtg atctccccgg aagccgccag ttcagccgcc
    56041 tcgtcgagat ctttcgccag tccttcgaac accagcgctc cggtgaccgc gcctttcatc
    56101 gcgccgcaca ttttttccca ggtgatcggc ggcccggcgt gaagaatggt tttcggcgtc
    56161 atgcccggca ccacgttaat cgcctgatca aaaccaatta gcaccggatg cgactggata
    56221 atgcgttcca gcgcctgctg gttagcggcg gcgattttgt ctgccagcgg cgaatcggca
    56281 atgttatcca gcgcctgcac cacctgcata ttgccctgcc ccggcggcgt ccagtcgagt
    56341 tgagtcactt ctacatgctg ctttttcagg tcatcgctaa acatggcgat gccgacgtta
    56401 ataacgttca atggttggct aaacagtgac tggctcatca ggcttcctcc cctttgcaga
    56461 taaattcacg cgccagcaat ccggtattgg tgctgctgct cgccagaatc actccggcat
    56521 caagcagcat ctggctttgt tgttctaacg atggcgtatc aagatcggta cccagcacat
    56581 aggcgagaat gatcaactcg cgtccttcgg cagcggcgat cgctttcgct tctttgatcg
    56641 cctcgatggt ggagccgacc ggatcttcat gcgatccaaa tccgagcaca aaatccatca
    56701 cgatcaccgc cacttctgga tcgcgcgcct cttcgatcaa gcgactgatg cggttggtgg
    56761 ggtcaatcat tggatgcggc ttgccgttgg tgaagtcgtc atcgccaaag tcgaggaagg
    56821 tgtgtttgat gctgcggttg atatctttca ggcggaattc cggatcgggc tgaatgttgc
    56881 tgtagacatc gccatgtttt tccatcaccg cgaacatggt ttcgtcgcac aacgtgccgc
    56941 cgcagaacag gccacgaatg tatttctgct gcggttgcag acgcgcacgc acatccgcaa
    57001 tcaacggctg gttaagcgta tgcaggtcga gattttcctg tttcacgccg gagagcatca
    57061 ccgcttttag cgccgcttct ttggtgccgc gagcaaactg tagcccctgc tcgtcaaccg
    57121 gcgtttcgcc acgaccgagg aagcagacca ccaccggctt gcggcaggcg cgggcgcgtt
    57181 ccagcacttt acgggccacc gcaggcgcgg gaggttttga aatcagcgcg ataatttcag
    57241 tttgcggatc gttttccagc atcccgatgg cgtcgagcat catcaggccg ccgattttct
    57301 cgctcaggtc gcgcccgccg gtgccgatga gttgcgaaac gccgccacca aattcatgaa
    57361 tgcggacgct cagctcctgg ctgccggtgc cggatgcgcc aacaataccg atgttgccgc
    57421 gacgcacggc attaccaaaa cagagcgccg cgccgttgat aatcgccgtg ccacagtctg
    57481 gccccatcat cagcagccct ttttcgtggg ccaactgttt gagcgccagt tcatcttcaa
    57541 ccgagacgtt atcggaaaac agcatcacgt taagatcgtt ttgcagcgcc tgacgcgctt
    57601 cgcgcgcggc aaacagaccg ttgaccgaaa tcaccgccag gttactttcc gggatatgct
    57661 ttttggcgct ggcaatagtg gcgtaacgcg cctcgtgcga gccgctttgc gctttggtgt
    57721 tgaacagttc ttcaatctcc accagcaact gctcgttgtc cgcccccgat ttaccattga
    57781 tgacaatcat caggtcgccg tttttcgcct gctccagctc cggcgtcagc agtcctaaat
    57841 ttttcagcac acctttgttc atttcggtcg ccatcgccac aaatgcctgc tcgacgccgt
    57901 cgagtttatt tgcacgcgtg gagatagaca tcagcgagac agaatcaaaa taggtattcg
    57961 gtttaatgac tattttaact gacataattc cctccgtagg tttggctcag cgcacaacca
    58021 tcggccatgc catacagcat gtcgcagccg gaactggagc caatatgttt aattttttct
    58081 attgcctgag ttgcgttccc agggatgtca taaataatgt tgtgaataaa atgatgaata
    58141 ttttcccggc agcgttgttg taatgcggct tccagcgtta tggcacttaa taacgtggta
    58201 ttatttctgc cgcgttgcaa gccgagataa aattcctctt tgtattttcc cgccggatgc
    58261 ccggaaataa ataaaataag tgccagacct gttaaataat cgtcggctga tggcgtaagg
    58321 ccaatcccta agcccatcat actggcgacc gctgaggcga tattttgttt atcgattact
    58381 gcctgtataa ctgcccgtcg tttaatatgt aattctttat ttaacgcctg ataaaacgga
    58441 ttatcgccgc gataattaaa cagggtttca ttttccccca ggtgctgatg aataatgtcg
    58501 tgccactgtt gccaggcgat aaggtgaaaa ttttcagcgt tcatttgcaa agttggggac
    58561 agccaacgct ggcaacgact catctctata tgaagatgtt gaccaaccgt aattccttga
    58621 tcgtcaaact gaaccttatc accatgccgg aacagaccat taaagtgagt gagtgccaac
    58681 cgacaactgt ttggcgcatt gtcgtactct tcacaaagca gcgtcagcaa ctgctggcga
    58741 gccgggagtg cgatatttac cgctctggca aagacctgtt caacctgtcc ttgcccccgc
    58801 tgcgccagaa aatccgcatc cgcagttagc gcccacacac accgtttttc tggtgtgttg
    58861 atagcaagcc tcccggttta tgcacctgcg gcaatcagta actgcgcaat ctcttcaaag
    58921 ccccgttccc gcgccagttc cagtggcgtt ttgccatatt tatcggtcag atgcgggctg
    58981 gcaccgtgtt ccagcaataa ctgcacaatc gcctgctgtt taatcccgcc atcgttaagc
    59041 acaatcgctt ccagtagtgg cgtccagcca acatggttgg tctggttaac gttaatttcc
    59101 gtatgcgcca aaagctcttt tacaatactt aaatggcctt tttcacaggc aggcgtcagg
    59161 ccgacaccgc caaaacgggt gacgcaatta aggtctggtt tagccggtaa aataattcgt
    59221 agcagcgtta aatcaccgtt cagacagcta attaaaaaag gatttaaaca ggtatgatct
    59281 tgcttattaa tatccgctcc ggcatcaatt aatgcctgaa cgcaagcata ttgctgatat
    59341 aaacttgcca gcgtaattgc cgttttcccc tgacgatcgc aggtattaat atcgacaccc
    59401 agcgcaaggc aggtttttac tttatcgata tcaccctgtt gtgcagccaa taaatagtcg
    59461 gcaggtagat tttttatact cattcacatt cccctggttt ttgtggctgt tttcgatgaa
    59521 ttgagaatag caacgataat ctgataaaag aacccgctta aatttaattc gccgtatttc
    59581 gcctttttgt tgaatgatgt tcaacaatct tttcgcagtg tgcggtagtg caatatttgt
    59641 ttctttgacc cgataccctt tcactcaatc aggttttatt tctctatagt ctgcacatgt
    59701 aagagtagct ttcgtccatg tctaaccaga ggggaacaag atgaacggac tcacggcaac
    59761 gggtgtcacc gtcggtattt gtgcagggct ttggcagtta gtgtcttcac atgtcgggct
    59821 ctctcaggga tgggaattat taggaaccat tggcttcgtc gccttttgca gtttttatgc
    59881 cgcaggcggc ggtaaatcgg ggtttatcag aagtctggcg gttaattatt ccgggatggt
    59941 gtgggcgttt ttcgccgcgc tgactgctgg ttggctggcc tctgtaagtg gactgtccgc
    60001 gttctgggcg agcgtaatta ctaccgtacc cttctcggcg gtggtggtct ggcaagggcg
    60061 tttctggtta ctgtcattta ttcccggtgg ctttctcggc atgacgctgt tttttgccag
    60121 cgggatgaac tggacggtga cgttactggg ttttctggcg ggtaactgcg tggggataat
    60181 ttcagaatat ggtggacaaa aattaagcga agcgacgact aaacgcgacg ggtattaaaa
    60241 gttattcaaa aatgattatc tatctttaac cgatacctct acagtcatag ctggagaaat
    60301 tcatagtaga taatggcgag aatggtttaa tgaactcaat ttttaccgaa gaaaacctgt
    60361 tggcatttac taccgctgcg cgttttggca gcttcagtaa agccgcagaa gagttgggtt
    60421 taaccacttc cgccattagc tacaccatca agcgtatgga gacggggctg gatgtggtgc
    60481 tgttcactcg cagtacccgc agcattgagt taacggagtc cgggcgttat tttttccgca
    60541 aagctaccga cctgctgaat gatttttatg ccatcaaacg tagcatagat actatttcgc
    60601 agggcataga agcgcgtgtg cgcatttgta ttaatcagct gctttatacg cccaaacaca
    60661 ccgcgcgctt attacaggtg ctgaaaaagc agtttcctac ctgccagatt accgtgacga
    60721 ccgaagtgta taacggcgtc tgggatgcga ttattaataa tcaggccaat atcgccatcg
    60781 gcgcacccga tacactactg gacggcggcg gtattgatta cactgaaatt ggcgcgattc
    60841 gctgggcttt tgctatcgcc ccggatcacc cgttagcctt tgtgccggaa cccatcgccg
    60901 aaagccaact gcgtctctac cctaatatta tggtggagga caccgcgcat acgattaata
    60961 aaaaagtggg ctggttgctg cacgggcagg agtcgattct ggtgccagat ttcaacacca
    61021 aatgtcagtg ccagatttta ggtgaaggga ttggtttttt gccggattac atggtccgtg
    61081 aggcgatgac gcaatccctg ctggttaccc gacaaatcca taatccgcgc caggattcgc
    61141 gcatgttact ggcgactcag cattctgcta cgggtcaggt gacgcagtgg attaaaaaac
    61201 aatttgcgcc aaatggcata ttaacgggga tctatcagga tttactgcat cgggagaatt
    61261 gataccactc aacctcccga tgcgctgtga atagcggttt acatcaacca cctgctttca
    61321 ttacccattc agagataaat ttattacccg gtactggcgg agagtacaaa tagccctgca
    61381 aaaagtgaac gcctttaccg atcattaaat ccgcctgctc ctgggtttct accccttccg
    61441 ccacgatact cagaccaggc ttacgcgcca gttcgacaat attgtccaca atatgaccgg
    61501 atatttcgtc gacactcgcc atttgcacaa atgacttatc gatcttaata aaatcgaccg
    61561 gaaacgcctg cagataacga taggtcgcat aacccgtacc aaagtcatcc agcgcaaagg
    61621 taatgttatg ctgatgaagg ctgtcaaata tcgctctggc ttctggcgtt accggaatag
    61681 ggttacgttc cgttagctcg agaaccagtt tgattttatc gtttcctaat ttcttgacca
    61741 ggttcaggca ttctttttca aatcccgcgg ccaaaaaaca acccgccgag acgttgatac
    61801 caatatggaa attatccggc agcaaatgtt ttaccggcat cagaatatcc gcagtctgtt
    61861 tcatcagttg gcgggtcatt atgacaataa gaccggatga ctccgccaga ggaataaact
    61921 gatccggcgg gatgattccc gtttgtggat gttcccagcg gacaagcact tcacagcccg
    61981 tcagtacccc agtctgcgcg cagaaaaccg gttggatcca cggtttaaat tcatggtttt
    62041 ccaacgccag actgatggct tcaggcgtga cgatatggtg acaacggtta tcagggatat
    62101 aaaaattatt tttatttccc gtgccactaa tgacttgcgg atgatattga aaaaagatat
    62161 cccgccagaa ggtaatatca ctctggatgc ctagtttttc atacagctgc ttcttttgat
    62221 gtgaaattgt ctttgcactg cgatttctgt actgtgagat ttctgtaacc gacattccct
    62281 ccattagcag cttgagtaaa agccgttctt tttcggacac gataccaggc aacgtcaaat
    62341 tcaccgttgc cccgaactct tccaaaaaaa cactcaaatc atgcgaagcc attgaacaca
    62401 ccttgattaa atcttgtgcg ccagctaaat ctgcgagtct tcactctgac ggataaagct
    62461 ggctgcaaga aaatgacaca gagagccgaa ttatctcccg ttcggctctc tctcctttta
    62521 ctcattattc agatcagaat tgccacttaa tgccaaccat tgcagaggtg tcattatatc
    62581 ccctatcggc aacctgaacg cccacattcc cccagacatt caggttggca tttagctgtc
    62641 cttccacccc ggtttttatc tcagcaatat ttcgggctcc atcctgagtg acggacacgc
    62701 catccatact ggtgctgaaa tccttactgt tatgcagcca gttcacttct acaaacggct
    62761 ggaactcgcg ggatttaccg tcatccattt tatggtggct cttcagccag gtttttacgc
    62821 caagtcgggt ttgaacattg ccatcaccgt tgctatgaac gagggttccg ttgctttcgc
    62881 ggtgcttatc ggctttgact cccatccagg taacctgtgc ttgcggctga acataccatt
    62941 cattacgcgt tccctggctg ccattaaatt cagctaattt gtgtttgtat ccagcttcca
    63001 gtgaagcggt aagtcctttt gatttatagg attcactttg taagtcatcc cctttcactg
    63061 tgttatcaaa ccagctgtac tgcgcccaac tgtcgagata cgcgccatta cgcgactcgt
    63121 catcggcata ccaggtggca tagaggcctg tgctatatcc gttcacactc gcttttgcac
    63181 gataaccggt tcgcgaggaa atggttttgc tgtcgctgtt gccatatccc gccatgaccc
    63241 caacatgcca gctgtcgctg ccgttttggc tccactgcgc gacatcgcct cccagttgca
    63301 gaacatagcg attgctttgg gttttcagct ggccgctgcc atcacgccat ttattatgac
    63361 caccttcatg gcgcatccac atagtggttt gtttctgctc acccgtcacc atgtcggtat
    63421 agtccgtatt acccagacgc tcatgcagac gcgtggtgaa catggtattc gctgctgcaa
    63481 ggttcgcaat gtagctaccc gcctccggtc gcaggtcatt atccacattc agatccggac
    63541 ccggcgtagg tgtcgggtta gggttcggct ctggattcgg gtttggctcc ggattcggca
    63601 tcgggtctgg ctccggctgc agttcaggag aatcactgcc gctggtcaga taccagttac
    63661 cactatttgc cccttgtcca cgcgcgagag tgtagtcata cgcccccgca acgatacgac
    63721 cagcctgaac aaattcgccc tcagacttac cgtctacatg gataagttcg ataccgttaa
    63781 gggttttcgc acctgtaccg ccagcgttat tcaccgtaac ggcagtcgtg ccgctagtat
    63841 cgccctcgac aaccaattta tcggttactg agtcatcgcc attcagtacc gtattcatta
    63901 ctagttgacc gttattaccg tggtagttcc ccttcaccac taacacattg ccagcttgct
    63961 gtcctgacat tccggtcatc agcgtaccgt tgttggtcag attgccgccc agggtgtaat
    64021 tcacagacgc agccgctctt ttttcagcgc tattattcaa cgccagagta ccgttgttct
    64081 caacgtttgc gctacccaat ccctgcgcat gacttaccag gacattagcc gaattagcaa
    64141 tgttcacatt cgccgtcagg ttgctgttat cgcccgcgat gtccagcgtg ccgccctgaa
    64201 tattcagctc tccgctcccc gttaacgaac cggttgaagt gcccccgttg gtaaccgtca
    64261 gactgccgcc atttaagctc agcagtgaat cggcgctgcc attgagcgtg cccacggtct
    64321 ggctgtgacc attcatatcc agttcagtct ctgccgccag acgcagttca tgggtattac
    64381 ccaacacgtt atcgttattc aacaaaagcg tcccactgcg cagatcggta acaccggtat
    64441 agtcgttgtc tttgttagaa agcgatacgg tctgaccctt ctggctgctg aatgccaggt
    64501 caccactgcc ggtaatcttt gcgctgagat cggcggcatt ctcgctttta ccgttagagc
    64561 tcagcaccag agcatcgctg tcggtagcgt gtaaatcaag ctgggtcagg ccgtaaccga
    64621 tatacagacc gtcattgttt accccactcg tcagacgata gtcgtaagtg ccgacggccg
    64681 ctttttgccc attctgctga atatcacgtt gggcgctgct ggagaggatt tgcccatttt
    64741 tatcctgcaa ttgcagattg cccgcatcgc ccagaacttc cgcaccttgc gcatcaacca
    64801 acttaatggt gctgttacca tcatcgactt cagtgagcga taacgcagag tcaatatcac
    64861 gagatactga gcttacaacg tcactgtcag aaacctgaat cacaccctcg ccgcgcaaat
    64921 ccagcgtttt actaacgttg accgtccctt ctgtcatctg tgcacctgct gtcagggcac
    64981 caaaatcaac ggttccgccg gagaatccca aagtgctaat ggagtgagcg cccttaccca
    65041 ctgttaccag gcttccctga cccgcagtga tcccgccttt ctgcagaaac tcagcattct
    65101 tttccagatt aaatgtcgtg ttctggaacg ccagtgaacc ctggaaccag tctgtataag
    65161 caaactcatc gaaggagaat tcgtgattcc ccatgtttat ttcaacaata cctctacctt
    65221 caagatcgtt atcaaatacc caatctttat tggcattgat ttgcagtttg ccatcaacat
    65281 ccaggtccgc acggccgatg ttcttgtctt cagaaataac cgcgacagca ccagaagcga
    65341 catcaatata tccgctgaat gcatagttat tgccggtcag tgccactgtc ccactgtcga
    65401 tacttattgt accattgccg gaaatcttat tggcaaaggt catgctatcg tcagccttca
    65461 ggtgtaattt cccgcggttg ttgatttctg ctgtacctat attttgggtg atccctaaag
    65521 cgccattttg ctcaattttg actttaccgc taaaggcgga gttatccccg gttaagtaag
    65581 tcgtaccatt gagcgcattg atatagccca ggccctcgct gtcactgaca atatccgcgg
    65641 aaatagtcgc gtttttatcc gtgtggttta aggtgatttt cttctcggat gtctttgccc
    65701 acacaaactc aattttttcg gcatcaataa tccctgcagc cttcgctgcg cttccttcct
    65761 gtgcacctaa cgctaactca gagttggtgc ttaaactaat agtcggcgca gagattttac
    65821 caccttccga aacggcaagt gttgtatcgg agttttgatc ataataaccc aggctaattt
    65881 tctcactggc attaacttca ccgccgttat taactgacag gtactgatta ccgccatatc
    65941 cgccgtaaac gcccataaag aaggtcccgg catttacctg actaccttcg ccgcttacat
    66001 tcccctgcgt ataagtcaga cgactggtgc ccttaacatg aattttgccg ccgttggaga
    66061 cattaaaata gccttgatca tgtctcatgc ctgagtttga ctcatacaaa tcatcagcat
    66121 ataactcgct gccagcattg gagacattca ggaagcccgt acctttcagc aaaaagttaa
    66181 gtgggccatt gattgttacc tttcctccgg taatatccaa cccaccacca gacgcgagag
    66241 tcagacgcgg acgggaagta tctgtcgcat tgattaccag attggcattg tctgtcaccg
    66301 taatacggta atcgatgctc tggttctctt tgccaaaatc aatagtttta tcctggttta
    66361 cgatgacatc atcggcgaat accagagatg aatataaact tatcgcgcta attaaaacag
    66421 cccgggactt ttttcctttt actctgctcc ccagctccga gcagacgctc caacagttac
    66481 gggacctgtt ccactttagc cgatatattt tattcatcac aatctccagc aatacatcca
    66541 aataaaacca agattgcata aacagcaacg cattaaatgc cattccggtt actgttcatc
    66601 gcatataaaa attcaaaaat tttgcatagc attggcaacc acatatctga gtatgtagtt
    66661 cagagggtaa taaaatagca agttactact gaaaacttat tttaagttaa cactcttaac
    66721 tttatcatta cattcccccc agtctctaat acctgcgcat tat
//
LOCUS       DAZFFG010000013        63180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFFG010000013 DAZFFG010000000
VERSION     DAZFFG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.013"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..805
                     /locus_tag="ACHJ7J_001089"
     CDS             <1..805
                     /locus_tag="ACHJ7J_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4999356.1"
                     /translation="SLQPYLVINKGAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIM
                     SITENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHH
                     GITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHTIINSKKFKTSRAISCR
                     SFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSK
                     HTGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            995..1108
                     /gene="yhiY"
                     /locus_tag="ACHJ7J_001090"
     CDS             995..1108
                     /gene="yhiY"
                     /locus_tag="ACHJ7J_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO4999357.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGIIR"
     gene            1171..2238
                     /gene="yhiI"
                     /locus_tag="ACHJ7J_001091"
     CDS             1171..2238
                     /gene="yhiI"
                     /locus_tag="ACHJ7J_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFO4999358.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     NLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2235..4970
                     /gene="rbbA"
                     /locus_tag="ACHJ7J_001092"
     CDS             2235..4970
                     /gene="rbbA"
                     /locus_tag="ACHJ7J_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO4999359.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIAHGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4970..6094
                     /gene="yhhJ"
                     /locus_tag="ACHJ7J_001093"
     CDS             4970..6094
                     /gene="yhhJ"
                     /locus_tag="ACHJ7J_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4999360.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6167..6442
                     /locus_tag="ACHJ7J_001094"
     CDS             6167..6442
                     /locus_tag="ACHJ7J_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4999361.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6439..6798
                     /locus_tag="ACHJ7J_001095"
     CDS             6439..6798
                     /locus_tag="ACHJ7J_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4999362.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6848..7708)
                     /locus_tag="ACHJ7J_001096"
     CDS             complement(6848..7708)
                     /locus_tag="ACHJ7J_001096"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO4999363.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(7740..8009)
                     /locus_tag="ACHJ7J_001097"
     CDS             complement(7740..8009)
                     /locus_tag="ACHJ7J_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO4999364.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEDE"
     gene            complement(7999..9507)
                     /locus_tag="ACHJ7J_001098"
     CDS             complement(7999..9507)
                     /locus_tag="ACHJ7J_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4999365.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9500..10858)
                     /locus_tag="ACHJ7J_001099"
     CDS             complement(9500..10858)
                     /locus_tag="ACHJ7J_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4999366.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(10935..11216)
                     /locus_tag="ACHJ7J_001100"
     CDS             complement(10935..11216)
                     /locus_tag="ACHJ7J_001100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4999367.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKN"
     gene            complement(11213..11686)
                     /locus_tag="ACHJ7J_001101"
     CDS             complement(11213..11686)
                     /locus_tag="ACHJ7J_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4999368.1"
                     /translation="MQGIQFQENFIQRLPAGLGVEEIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(11711..12457)
                     /locus_tag="ACHJ7J_001102"
     CDS             complement(11711..12457)
                     /locus_tag="ACHJ7J_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4999369.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(12656..13057)
                     /gene="nikR"
                     /locus_tag="ACHJ7J_001103"
     CDS             complement(12656..13057)
                     /gene="nikR"
                     /locus_tag="ACHJ7J_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO4999370.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(13063..13869)
                     /gene="nikE"
                     /locus_tag="ACHJ7J_001104"
     CDS             complement(13063..13869)
                     /gene="nikE"
                     /locus_tag="ACHJ7J_001104"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO4999371.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(13866..14630)
                     /gene="nikD"
                     /locus_tag="ACHJ7J_001105"
     CDS             complement(13866..14630)
                     /gene="nikD"
                     /locus_tag="ACHJ7J_001105"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO4999372.1"
                     /translation="MPQQIELRDIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAAALGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLRTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(14630..15463)
                     /gene="nikC"
                     /locus_tag="ACHJ7J_001106"
     CDS             complement(14630..15463)
                     /gene="nikC"
                     /locus_tag="ACHJ7J_001106"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO4999373.1"
                     /translation="MNFFISSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMTCLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(15460..16404)
                     /gene="nikB"
                     /locus_tag="ACHJ7J_001107"
     CDS             complement(15460..16404)
                     /gene="nikB"
                     /locus_tag="ACHJ7J_001107"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO4999374.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(16404..17978)
                     /gene="nikA"
                     /locus_tag="ACHJ7J_001108"
     CDS             complement(16404..17978)
                     /gene="nikA"
                     /locus_tag="ACHJ7J_001108"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4999375.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(18089..18676)
                     /gene="acpT"
                     /locus_tag="ACHJ7J_001109"
     CDS             complement(18089..18676)
                     /gene="acpT"
                     /locus_tag="ACHJ7J_001109"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO4999376.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(18678..19907)
                     /locus_tag="ACHJ7J_001110"
     CDS             complement(18678..19907)
                     /locus_tag="ACHJ7J_001110"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO4999377.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(19904..20635)
                     /locus_tag="ACHJ7J_001111"
     CDS             complement(19904..20635)
                     /locus_tag="ACHJ7J_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO4999378.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(20635..21099)
                     /locus_tag="ACHJ7J_001112"
     CDS             complement(20635..21099)
                     /locus_tag="ACHJ7J_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO4999379.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(21096..22265)
                     /locus_tag="ACHJ7J_001113"
     CDS             complement(21096..22265)
                     /locus_tag="ACHJ7J_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO4999380.1"
                     /translation="MIYISAVGMINALGNNLDEISANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(22267..22851)
                     /locus_tag="ACHJ7J_001114"
     CDS             complement(22267..22851)
                     /locus_tag="ACHJ7J_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO4999381.1"
                     /translation="MIKPTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISVWQPQLPTGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(22848..25166)
                     /locus_tag="ACHJ7J_001115"
     CDS             complement(22848..25166)
                     /locus_tag="ACHJ7J_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO4999382.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLIRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIHQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(25135..25740)
                     /locus_tag="ACHJ7J_001116"
     CDS             complement(25135..25740)
                     /locus_tag="ACHJ7J_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO4999383.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(25737..26159)
                     /locus_tag="ACHJ7J_001117"
     CDS             complement(25737..26159)
                     /locus_tag="ACHJ7J_001117"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO4999384.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(26163..27839)
                     /locus_tag="ACHJ7J_001118"
     CDS             complement(26163..27839)
                     /locus_tag="ACHJ7J_001118"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4999385.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVSKQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLAVAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(27830..28183)
                     /locus_tag="ACHJ7J_001119"
     CDS             complement(27830..28183)
                     /locus_tag="ACHJ7J_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO4999386.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(28170..29531)
                     /locus_tag="ACHJ7J_001120"
     CDS             complement(28170..29531)
                     /locus_tag="ACHJ7J_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFO4999387.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLDESFWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVANSPQIATQSFTFSAIAPEAFIELFTSGSTGQPKRVIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQLAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPFPGMQFQAEGDSFRLFSPLIAEDNGLLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(29528..30109)
                     /locus_tag="ACHJ7J_001121"
     CDS             complement(29528..30109)
                     /locus_tag="ACHJ7J_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999388.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLVRRQVMKRDRA"
     gene            complement(30114..30365)
                     /locus_tag="ACHJ7J_001122"
     CDS             complement(30114..30365)
                     /locus_tag="ACHJ7J_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4999389.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(30377..30634)
                     /locus_tag="ACHJ7J_001123"
     CDS             complement(30377..30634)
                     /locus_tag="ACHJ7J_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO4999390.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(30609..31430)
                     /locus_tag="ACHJ7J_001124"
     CDS             complement(30609..31430)
                     /locus_tag="ACHJ7J_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO4999391.1"
                     /translation="MSKLLSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(31427..32149)
                     /locus_tag="ACHJ7J_001125"
     CDS             complement(31427..32149)
                     /locus_tag="ACHJ7J_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO4999392.1"
                     /translation="MKFTLNITDWQALAPGLSEAQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDTFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDEWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNADTFSLPGERVQWRWSRG"
     gene            complement(32190..33248)
                     /locus_tag="ACHJ7J_001126"
     CDS             complement(32190..33248)
                     /locus_tag="ACHJ7J_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4999393.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYAASVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(33317..33694)
                     /locus_tag="ACHJ7J_001127"
     CDS             complement(33317..33694)
                     /locus_tag="ACHJ7J_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4999394.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(34086..35135)
                     /gene="yhhT"
                     /locus_tag="ACHJ7J_001128"
     CDS             complement(34086..35135)
                     /gene="yhhT"
                     /locus_tag="ACHJ7J_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4999395.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            35267..36484
                     /gene="yhhS"
                     /locus_tag="ACHJ7J_001129"
     CDS             35267..36484
                     /gene="yhhS"
                     /locus_tag="ACHJ7J_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4999396.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMAVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(36488..37045)
                     /gene="dcrB"
                     /locus_tag="ACHJ7J_001130"
     CDS             complement(36488..37045)
                     /gene="dcrB"
                     /locus_tag="ACHJ7J_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO4999397.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(37118..37783)
                     /gene="yhhQ"
                     /locus_tag="ACHJ7J_001131"
     CDS             complement(37118..37783)
                     /gene="yhhQ"
                     /locus_tag="ACHJ7J_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO4999398.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            38004..38249
                     /gene="tusA"
                     /locus_tag="ACHJ7J_001132"
     CDS             38004..38249
                     /gene="tusA"
                     /locus_tag="ACHJ7J_001132"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO4999399.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(38332..40530)
                     /gene="zntA"
                     /locus_tag="ACHJ7J_001133"
     CDS             complement(38332..40530)
                     /gene="zntA"
                     /locus_tag="ACHJ7J_001133"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO4999400.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPENATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(40604..41230)
                     /gene="yhhN"
                     /locus_tag="ACHJ7J_001134"
     CDS             complement(40604..41230)
                     /gene="yhhN"
                     /locus_tag="ACHJ7J_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO4999401.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            41371..41730
                     /gene="yhhM"
                     /locus_tag="ACHJ7J_001135"
     CDS             41371..41730
                     /gene="yhhM"
                     /locus_tag="ACHJ7J_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO4999402.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGKQ"
     gene            complement(41733..42002)
                     /gene="yhhL"
                     /locus_tag="ACHJ7J_001136"
     CDS             complement(41733..42002)
                     /gene="yhhL"
                     /locus_tag="ACHJ7J_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO4999403.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(41992..42588)
                     /gene="rsmD"
                     /locus_tag="ACHJ7J_001137"
     CDS             complement(41992..42588)
                     /gene="rsmD"
                     /locus_tag="ACHJ7J_001137"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO4999404.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            42738..44234
                     /gene="ftsY"
                     /locus_tag="ACHJ7J_001138"
     CDS             42738..44234
                     /gene="ftsY"
                     /locus_tag="ACHJ7J_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO4999405.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETQAL
                     AAEAAEEAVMVVPLAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            44237..44905
                     /gene="ftsE"
                     /locus_tag="ACHJ7J_001139"
     CDS             44237..44905
                     /gene="ftsE"
                     /locus_tag="ACHJ7J_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO4999406.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            44898..45956
                     /gene="ftsX"
                     /locus_tag="ACHJ7J_001140"
     CDS             44898..45956
                     /gene="ftsX"
                     /locus_tag="ACHJ7J_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO4999407.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            46201..47055
                     /gene="rpoH"
                     /locus_tag="ACHJ7J_001141"
     CDS             46201..47055
                     /gene="rpoH"
                     /locus_tag="ACHJ7J_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO4999408.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTAAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            47326..48429
                     /gene="livJ"
                     /locus_tag="ACHJ7J_001142"
     CDS             47326..48429
                     /gene="livJ"
                     /locus_tag="ACHJ7J_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO4999409.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(48629..49012)
                     /gene="panM"
                     /locus_tag="ACHJ7J_001143"
     CDS             complement(48629..49012)
                     /gene="panM"
                     /locus_tag="ACHJ7J_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO4999410.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            49436..50545
                     /gene="livK"
                     /locus_tag="ACHJ7J_001144"
     CDS             49436..50545
                     /gene="livK"
                     /locus_tag="ACHJ7J_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO4999411.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            50593..51519
                     /gene="livH"
                     /locus_tag="ACHJ7J_001145"
     CDS             50593..51519
                     /gene="livH"
                     /locus_tag="ACHJ7J_001145"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO4999412.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            51516..52793
                     /gene="livM"
                     /locus_tag="ACHJ7J_001146"
     CDS             51516..52793
                     /gene="livM"
                     /locus_tag="ACHJ7J_001146"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO4999413.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAVFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            52790..53557
                     /gene="livG"
                     /locus_tag="ACHJ7J_001147"
     CDS             52790..53557
                     /gene="livG"
                     /locus_tag="ACHJ7J_001147"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO4999414.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            53559..54272
                     /gene="livF"
                     /locus_tag="ACHJ7J_001148"
     CDS             53559..54272
                     /gene="livF"
                     /locus_tag="ACHJ7J_001148"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO4999415.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            54391..55137
                     /locus_tag="ACHJ7J_001149"
     CDS             54391..55137
                     /locus_tag="ACHJ7J_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4999416.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQNKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            55497..56813
                     /gene="ugpB"
                     /locus_tag="ACHJ7J_001150"
     CDS             55497..56813
                     /gene="ugpB"
                     /locus_tag="ACHJ7J_001150"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO4999417.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            56911..57798
                     /gene="ugpA"
                     /locus_tag="ACHJ7J_001151"
     CDS             56911..57798
                     /gene="ugpA"
                     /locus_tag="ACHJ7J_001151"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO4999418.1"
                     /translation="MSSSRPVFRARWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            57795..58640
                     /gene="ugpE"
                     /locus_tag="ACHJ7J_001152"
     CDS             57795..58640
                     /gene="ugpE"
                     /locus_tag="ACHJ7J_001152"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO4999419.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            58642..59712
                     /gene="ugpC"
                     /locus_tag="ACHJ7J_001153"
     CDS             58642..59712
                     /gene="ugpC"
                     /locus_tag="ACHJ7J_001153"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO4999420.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            59709..60452
                     /gene="ugpQ"
                     /locus_tag="ACHJ7J_001154"
     CDS             59709..60452
                     /gene="ugpQ"
                     /locus_tag="ACHJ7J_001154"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4999421.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(60439..60879)
                     /gene="yhhA"
                     /locus_tag="ACHJ7J_001155"
     CDS             complement(60439..60879)
                     /gene="yhhA"
                     /locus_tag="ACHJ7J_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO4999422.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            60999..62732
                     /gene="ggt"
                     /locus_tag="ACHJ7J_001156"
     CDS             60999..62732
                     /gene="ggt"
                     /locus_tag="ACHJ7J_001156"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO4999423.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAHEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(62770..63054)
                     /gene="yrhB"
                     /locus_tag="ACHJ7J_001157"
     CDS             complement(62770..63054)
                     /gene="yrhB"
                     /locus_tag="ACHJ7J_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO4999424.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKAAFGLP"
BASE COUNT        14754 a        16500 c        17379 g        14547 t
ORIGIN      
        1 aagtcttcag ccatatttag ttattaataa aggcgcagtg acgccatgta cgtttctggg
       61 tgaccaaacc ggcgatcgat ttagtaatat ctgtggcgat caatttatca tcgatttgtt
      121 aaaacgcata atgagtatca ctgaaaatgt tcatgtatta gctggaaatc atgaaacgaa
      181 ttgcaatggt aactatatgc agaacttcac gcgtatgaaa ccacttgatg aggataccta
      241 cgacgggata aaagattatc ctgtatgttt ctatgacccc aaatacaaaa taatggcaaa
      301 tcatcatggg ataacttttg atgatcagcg aaaacgttac attataggac cgataacagt
      361 ctctattgat gaaatgacca atgctctcga tccggtcgaa ttagcggaaa ttattaataa
      421 aaagcatcat actataatta atagtaaaaa gtttaaaact agccgggcta tctcttgtcg
      481 atcctttaat cgttattttt ctgtttcgac agattacaga cctaaacttg aagcactatt
      541 agcatgctca caaatgttag gtataaatca agttgttgca cataacggta atggtggaag
      601 agaacgcata ggtgaaacgg ggacagtttt aggtcttaac gcccgtgaca gtaaacatac
      661 gggaagaatg ttcagcatgc ataattgcca aattaaccct ggtgcggggc ctgagataac
      721 cactccctgg aagtcttacc aacatgaaaa aaacagaaat ggacttatgc ctttgattag
      781 aaggcgcaca atgttacagc tctgaaccat agacaacatg aatattaatg ttactatctg
      841 gttgagactg ttgataaaac gtaataagga atgctctgtt cctgcaactg aacaggcgtg
      901 aaacgctatc caacaggatg gataccgttt tgtcagccag ttttatagat cacatttatg
      961 agtatgcgtg tttcatttac ggccaggaat gctgatgatg acaacattac tacccgtttt
     1021 cactaagcct tccccgttgg cgctcaatgc tctgcgcgct ggtcgtattt gtcgtttcct
     1081 tcttatcccg gatggaataa tccgctgaac gtcgttcggc ggcagttgta acacgcgtcg
     1141 tttgcttaca gggattatgg cgggtcgatt atggataaga gtaagcgcca tctggcgtgg
     1201 tgggttgtcg ggttactggc ggtggcggct atcgtggcgt ggtggctgtt gcgcccggca
     1261 ggtgtgccgg aaggctttgc cgtcagcaat gggcgcattg aagcgacgga agtggatatc
     1321 gccagcaaaa ttgccgggcg tatcgacacc attctggtga aagaaggcca gtttgttcgc
     1381 gaaggcgaag tgctggcgaa gatggatact cgcgtgttgc aggagcagcg actggaagcc
     1441 atcgcgcaaa tcaaagaggc acaaagcgcc gttgccgccg cgcaggcttt gctggagcaa
     1501 cgccaaagcg aaactcgtgc cgcacagtcg ctggttaatc aacgccaggc agaactggac
     1561 tccgtagcaa aacgtcatac gcgttcccgt tcactggccc aacgaggggc tatttctgcg
     1621 caacagctgg atgacgaccg cgccgccgct gagagcgccc gagctgcgct ggaatcagca
     1681 aaagcccagg tttcggcttc taaagccgct atagaagcgg cacgcaccaa tatcattcag
     1741 gcgcaaaccc gcgtcgaagc cgcacaagcc actgaacgcc gcattgccgc agatatcgat
     1801 gacagcgaac tgaaagcccc gcgtgacgga cgcgtgcagt atcgggttgc cgagccaggg
     1861 gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg atctcagcga cgtctatatg
     1921 accttcttcc tgccaaccga acaggcgggc acgctgaaac tgggcggtga agcccggctg
     1981 atcctcgatg ccgcgccaaa tctgcgtatt cctgcaacca tcagttttgt cgccagtgtc
     2041 gcccagttca cgccaaaaac tgtcgaaacc agcgatgaac ggctgaaact gatgttccgc
     2101 gtcaaagcgc gtatcccacc ggaattactc cagcagcatc tggaatatgt caaaacaggc
     2161 ttgccgggcg tagcgtgggt gcgggtgaat gaagaacttc cgtggcctga cgacctcgtg
     2221 gtgaggttgc cgcaatgacg catctggaac tggttcccgt cccgcctgtc gcgcaactgg
     2281 cgggcgtgag ccagcattat ggaaaaaccg ttgcgctgaa caatatcaca ctcgatattc
     2341 cggcccgctg tatggtcggg ctgattggtc cggatggcgt cgggaagtcg agtttgttgt
     2401 cgttgatttc cggagcccgc gtcattgagc agggcaatgt gatggtgctg ggcggcgata
     2461 tgcgcgaccc gaagcatcgc cgcgacgtct gcccgcgcat cgcctggatg ccgcaggggc
     2521 tgggcaaaaa cctctaccac accttgtcgg tgtatgaaaa cgtcgatttt ttcgctcgcc
     2581 tgttcggtca cgacaaagcg gagcgggaag tccgaattaa tgagctgctg accagcaccg
     2641 ggttagcccc gtttcgcgat cgtccggcag ggaaactctc cggcgggatg aagcaaaaac
     2701 tggggctgtg ctgtgcgtta atccacgacc cggaactgtt gattcttgat gagccaacaa
     2761 cgggggttga cccgctctcc cgcgcccagt tctgggatct gatcgacagt attcgccagc
     2821 ggcagagcaa tatgagcgtg ctggtcgcca ccgcctacat ggaagaggcc gaacgcttcg
     2881 actggctggt agcgatgaat gccggagaag tgctggcaac tggcagcgcc gaagagctgc
     2941 ggcagcaaac gcaaagcgcc acgctggaag aggcgtttat aaatctgtta ccgcaagcgc
     3001 aacgccaggc gcatcaggcg gtagtgatcc caccgtatca acctgaaaac gcagagattg
     3061 ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt cgttgccgtt gatcacgtta
     3121 atttccgcat tccacgcggg gagatttttg gttttcttgg atcgaacggc tgcggtaaat
     3181 ccaccaccat gaaaatgctc accggactgc tgcccgccag cgaaggtgag gcgtggctgt
     3241 ttgggcaacc ggttgatcca aaagatatcg atacccgccg tcgggtgggc tatatgtcgc
     3301 aggcgttttc gctctataac gaactcaccg tgcggcaaaa ccttgagtta catgcccgtt
     3361 tgtttcacat cccggaagcg gaaattcccg cgcgagtggc tgaaatgagc gagcgtttta
     3421 aactcaacga cgttgaagat gttctgccgg agtcattgcc gctcggcatt cgccagcggc
     3481 tttcgctggc ggtggcggtg attcatcgcc cggaaatgtt aatcctcgat gagcctactt
     3541 ctggcgtcga tccggtggcg agggatatgt tctggcagtt gatggtcgat ctctcgcgcc
     3601 aggataaagt gactatcttc atctccaccc actttatgaa cgaagcggaa cgttgcgacc
     3661 gcatctcact gatgcacgcc ggaaaagtgc ttgccagtgg tacaccgcag gaactggttg
     3721 agaaacgcgg agccgccagt ctggaagagg catttatcgc ctatttgcag gaagcggcag
     3781 ggcagagcaa cgaagccgaa gcgccgcccg tggtacacga caccacccac gccccgcgtc
     3841 agggatttag cctgcgccgt ctgtttagct acagccgtcg tgaagcactg gaactgcgcc
     3901 gcgatccggt ccgttcgacg ctggcgctga tgggaacagt gatcctgatg ctgataatgg
     3961 gttacggcat cagtatggat gtggaaaacc tgcgctttgc ggtgctcgac cgcgaccaga
     4021 ccgtcagtag ccaggcgtgg acgctcaatc tctccggttc ccgttacttt atcgaacagc
     4081 cgccgctcac cagttatgac gagcttgatc gtcggatgcg tgcgggcgat atcacggtgg
     4141 caattgagat ccctcccaat ttcgggcgcg atatcgcgcg tggtacgcct gtggaactcg
     4201 gtgtctggat cgacggtgcg atgccgagcc gcgccgaaac tgtaaaaggt tacgtgcagg
     4261 ccatgcacca gagctggcta caggatgtgg cgagccgaca atccacaccc gccagccaaa
     4321 gcgggctgat gaatattgag acgcgctatc gctataaccc ggacgtaaaa agcctgccag
     4381 cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat cccgtcaatg ctaagcgccc
     4441 ttagcgtggt gcgggaaaaa gagctagggt cgattatcaa cctttacgtg acccccacca
     4501 cccgtagcga gtttttgctt ggcaaacagc tgccgtacat cgcgctgggg atgctgaact
     4561 ttttcctgct ctgcggcctg tcggtgtttg tgtttggcgt accgcataaa ggtagtttcc
     4621 tgacgctcac cctggcggcg ctgctgtata tcatcatcgc caccggaatg gggctgctga
     4681 tctccacctt tatgaaaagc cagattgccg ccattttcgg tacggcgatt atcacgttga
     4741 tcccggcgac acagttttcc gggatgatcg atccggtagc ttcgctggaa gggccgggac
     4801 gttggatcgg cgaggtttac ccgaccagtc attttctgac tatcgcccac gggacgttct
     4861 cgaaagcgct ggatctgact gatttgtggc agctttttat cccgttgctg atagccatac
     4921 cgctggtgat gggcttaagc atcctgctgc tgaaaaaaca ggagggatga tgcggcattt
     4981 acgcaatatt tttaatctgg gtatcaaaga gttgcgcagt ctactcggtg ataaagcgat
     5041 gctgacattg attgtcttct cgtttacggt gtcggtgtat tcgtcagcga ccgttacgcc
     5101 aggatcgttg aacctggcgc cgatcgccat tgccgatatg gatcaatcgc agttatcgaa
     5161 tcggatcgtt aacagcttct atcgcccgtg gtttttgcca ccggagatga tcaccgccga
     5221 tgagatggat gccggactgg acgccgggcg ttataccttt gcgataaata ttccgcctaa
     5281 ttttcagcgt gatgtcctcg ccggacgcca gccagatatt caggtgaacg tcgatgccac
     5341 gcgcatgagc caggcattta ccggcaatgg gtatatccag aatattatca acggtgaagt
     5401 gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg ttggtatcgc tggaaacccg
     5461 gatgcgcttt aacccgaacc tcgatcccgc gtggtttggc ggggtgatgg cgatcatcaa
     5521 caacattacc atgctggcga ttgtattgac cggatcggcg ctgatccgcg agcgtgaaca
     5581 cggcacagtg gaacatttac tggtgatgcc gataacgccg tttgagatca tgatggctaa
     5641 ggtctggtcg atggggctgg tggtgctggt ggtatcggga ttatcgctgg tgctgatggt
     5701 gaaaggcgtg ctgggcgtac cgattgaagg ctcgatcccg ctgtttatgc tgggcgtggc
     5761 gctcagtctg tttgccacca cgtcaatcgg catttttatg gggacgatag cgcgttcaat
     5821 gccgcaactg gggctgctgg tgattctggt gctgctgccg ctgcaaatgc tttccggtgg
     5881 ctccacgccg cgcgaaagta tgccgcagat ggtgcaggac attatgctga ccatgccgac
     5941 gacacacttt gttagcctcg cgcaggccat tctctaccgg ggtgccggat tcgaaatcgt
     6001 ctggccgcag ttcctgacgc tgatggcaat tggcggcgcg tttttcacca tcgcactgct
     6061 gcgattcagg aagacgattg ggacaatggc gtaaggaagg gaaacttgac ttcatactgt
     6121 gatgtcatac tattgatgtc ataaagtgaa gtcataagga catgccatga gaaccaaggt
     6181 acaggcttta cggaagaaac aaaaaaatac attggatcaa atatttaaaa ctcctgttcc
     6241 tcaagggatc aagtggtctg atatagagtc acaggttaaa gcattaggcg gagaaattaa
     6301 ggaaggaaga ggttcgcgtt gtaagttcat actaaatatg agcgttgcgt gtttccatcg
     6361 gcctcatccg tcgccagata ccgataaagg cgctgtagaa agcgtgcgtg actggttact
     6421 aagtataggg gtaaaaccat gatcaaactg aagacgccaa actctatgga gattgccggg
     6481 caacctgctg ttatcactta cgtgccagaa ctgaatgcat ttcgtggcaa attcctgggc
     6541 ttgtcaggtt attgtgattt tgtctctgac agcattcagg ggctgcaaaa agaaggggaa
     6601 ctttccctgc gagagtatct tgaggattgt aaagctgcgg gtattgagcc gtatgcgcgt
     6661 acagaaaaga ttaagacctt tacgttacgc tatccagaat cgctgagtga acgccttaat
     6721 aatgcggctg cgcagcaaca agtctctgtt aatacctata ttattgaaac actgaatgag
     6781 cgtttgaatc atttataact tatagcggcc caacaatttc tgttgcgccg ctatattttt
     6841 gtggctctta agatgccttt ctaaccaacc gatgcccctc attcatcatc aggattttgc
     6901 tatacacatc ttcctcgacg gcgttttttg ctgatgccat gactcgtgcc agattcgcct
     6961 cgttgtgatt attcacatac gctttgccga cggcggtgat aaacgctttg cgcagatcgc
     7021 tggcgatatt tactttagcg acgcgataat taacaaagct gcgtaatatc tctggggcga
     7081 taccggagcc gccgtgaata accaacggca ccggacagac ttcggcaatg cgcttcagca
     7141 gggggatatc gatacgcggt atatcgtcca ggccatgaac gttgccaata gaaactgcca
     7201 gcatgtcgca accggtacgc tcgacaaagg tcttcacctt ttccggttcc gttttgcaat
     7261 cggcttcgct gacgtgatca tcttctttgc cgagaatcgc ccccagctcc gcttcaaccg
     7321 gtacgccata ggatttacag aaatcgacag cttcctgggt gaaagcgata ttctcttcaa
     7381 aagagaatgc ggcaccgtca atcatgaccg aggtaaagcc cgcgcgcacc gcctgtttaa
     7441 catcctcctg ggttttcccg tgatccagat gaagactgac cggaacatcc attttatcgg
     7501 cgtgacgttt cacgatttca tatatgtaat cgtatcccga cagttgtgca ttggttggcg
     7561 caatctgaat gaagttgggt aatcccgaac gctcaatagc gtttaaaatt gaaattgtcg
     7621 tctccagatt ggtggtatta aatgcgcccg ctaatacccg tttttcctga atgcggtcga
     7681 gcagtatccg tccattaact aatggcatat tgaaaacctc atcttttaat atcagttaat
     7741 tattcatctt caccgaagcg gtcgcgaaat aagtcggtga gagtctgtat ggcctcgttt
     7801 tcatcatcac cgtcgcagcg tatttccacc gtttttccgc cttttattcc ggcgcgcata
     7861 atattcatca tgctggtggc attaatatca ttaccctcat agtgcagcgt cactttacag
     7921 ctatattttt tggcggcttt cgccagacag gccgccggac gtgcatgcag gccagtactg
     7981 tttctgacct ccacggtttt attcaacata gtgttcctcc cgatgatgta atggcatcac
     8041 gccttcttgt tgtaatttca actgatacca gcttttccat ttgtgctgat aacgctgtaa
     8101 ttgatggtat ctcgcccgat tcggtaacaa acgttgggct atagcgactg gcggcggtgg
     8161 aatatccggt gcggcggtta ataatgctcc gacagaaacc gcctcgttaa atgcaattgc
     8221 ttcaaccgga cgctgtaaaa tatccgcctt taattgcagc caaagaggat tatgggttgc
     8281 cggaccgatc acttttaatg cgctggtgcc aggcacgtta aaacaggcga agcagttggc
     8341 aaattccatg gttaatccca gtgatacgct ggcaattaac atgtcgatac ttaacgtatc
     8401 gccaagccca taaaataatc cacgggtatg gcgattttta tagggcgaac ccgaaccgcg
     8461 aagatgtgga ataaagacgg gaatgtcatc gagcaaccaa ttccccgcca gataatcagc
     8521 atgtccgcga gtaagtgatg cggcgatctc ctcatcggtt agccggaacg tgttgcgcag
     8581 ccactccagc gcgaaaccgc ccacgggcag cgacgcaaac agggtgaact ggttggcaag
     8641 tgagtaacag ccgtttgcca gcttgtcacg tttggcctgt tcatccagcg tcggttgtgt
     8701 atccagttgc agaatgcctt ccgtggtccc cgtcgagtta aggatatcgc ctggcatcat
     8761 ctgcaacgct cgcgcaccca ccatatggtc atgtccggcc agcgtcacgc tgaccggttg
     8821 cgagaaccca agcgtcttgc aaagtgactc gctgacccat ccggcgtgct cgcctggctg
     8881 caccaatggc gcaaatgccg tacagggaac gtgtaacaac gccgccgctt tcgctgacca
     8941 ttcgcctttg cgcacgtcca gacacagcgt gcggctggct aaggtgatat ccgtgcgctg
     9001 ttcgccagtc agccgccaga gcagcacttc cggcgcatgt agccagcgca ggccgcggcg
     9061 attacgcagc gggtaatgtt ccagcaacca gcgcattttg aaggcagagt aattgctgtg
     9121 tagtggtaga ccgcagatgt cataaagtgc cgcactgtct gcctcgctaa gcgttgccag
     9181 atactcttca ccgcgacggt cataccatgc cagcattggc gtcaggatct cgccatgctc
     9241 gtcaaggaac acgcctgatt cgccaaaact ggcaatgctg atccgtctga ctggcagcgg
     9301 cgaagcggcg ttcagttgcg ctatcgcctg gcggacctcc tgccagaggg cgtcgatatc
     9361 gaaatcgaca tcgccctgtg gggagatctg ctttgccgtc acgaatttat gcgcgcccaa
     9421 cgtcgttgcg tccaggcagg aaaagcaggt gactttgcaa ttggtggtgc caatatcgat
     9481 aacgatagct gcgctgttat caggcataaa ccgtgctccg cgtcattcgg aaaatgccat
     9541 acagtaccag ggcgataacc ggcaccagac caatggcgat gatattgccg ttgaagattt
     9601 ccagcagcca gaagcggaac gggttaccgc catcaaggaa actggaaacc tggccgctgg
     9661 agccttcggc aaatttgaag ttaacgtctt tcgccatgcc ggtaataaac ggtgcgattt
     9721 tagtcgcaat ccacaggtcg gcaataatca ccgggatcgc cgccagaacg gtgcggaaaa
     9781 tattgccacg gctggctaaa gcaatcatcg acgccattac cgccaggttg gcaaggtcgc
     9841 caagcggcag gacttcatta cctgggagga caaacgcgat aaagaccgag attggcgtta
     9901 gcagcagggc ggtggagata atcgccggat tacctacggc aacggcgata tccagtccga
     9961 tatagagatc gtcacggtca gggtatttgg cattgagata ctttttaatg gcttcagaca
    10021 gcggtaataa accttccatc aggatgcgaa ccatgcgtgg caggatgaac atcacaccgc
    10081 caatgctgat gccaagcagc aacacttttt tgaaatcgta tcctgcaatc acgccgagca
    10141 gtatgcccag aatagtgcca accatcatcg gttcgccgaa gatgccaaac tttttctgta
    10201 cggtttccgg gtcgatatga atacgattga ggccggggat atggtcgatg attttgtcga
    10261 ccagcagacc gaccgggaag aacaccaccg aagagagcgt cggcaatgag atcccttcca
    10321 gtccaaagta tttttgtacc agtggcgcag accagtcggc gagtttaagt actaccaccg
    10381 cagcaatcgc cgccgccagc aaaccaaaga acaggctgcc ggtgctgtaa taaaccaacg
    10441 taccagcaag ggcaaaatgc cagtagttcc acatatctac gtcaacggtg cgggttttat
    10501 ttaacgccag catcgccacg ttaatcagaa tggtcagggg aataacaaac ggggcaattg
    10561 gcgaacccca tgtaatagcg gaaagcggcg gccagcctaa atctaccacc ggtaaattaa
    10621 ttccggtgcg ttcaaccatc gcctgggccg ctggcccgac attgctggtc agaatatcga
    10681 aaatggcata aataccgaca aaaccaatgc cgactgttaa tgacgaacgt aaggctttag
    10741 cgggttttac tttaaaacag agagcaagaa taaataacac caacggcaac attaccgtcg
    10801 ggccaaatcc caatatatat tgcacaatat tatagagggt atgcgcgata tcgttcataa
    10861 taaacctctt tttcaggcga gagcgcacct cgcgttagcg cctgatggag tgggttgaaa
    10921 ttaattatta ttaattaatt tttaaggatc gaaacaattt gcgccagtac tttctcttcg
    10981 ccaataccgg taataatcgg caagccgtta actaccggga cgtcgagttc ataaggcact
    11041 ttagtcgttg agactaccag tgctacgcca tcagtatttc caggtaaaga ggcaacgttg
    11101 gtttgggaat aattgacgtt aagaccgtgc tctttacaat attccatgac tttttctgtt
    11161 accgccgtgg atgtggcaat acccgtggcg cagacaaata aaatggtttg actcatattt
    11221 cacctcgttc tttcattttg tttaagatta ctgtgtgtat ttctgttgtt tccatcgcaa
    11281 gcagggcttg cataaagggc gtatcctgaa tcatttccat aatccagccc agggcattta
    11341 attgtttatt actttcgctt aaggcgagca aaaaaatcac cctgacaggt acggggtcag
    11401 gatcgccgcc catatcggca aactctaccg gttccggcag gatgccgacg gcaattgcat
    11461 tgtgcctgac atgtttatgg tccgtgtgcg gaatagcgac gcacggtggt tcggtaggca
    11521 gccccgttgg gtacgtcgcc tcacgtttca gtacgtgatc ggcaaaatca gggaccacca
    11581 actcggcggc aaccaacggt tgcgctaact gacggatgat ctcttcaacg cctaaccctg
    11641 ccggaagtcg ttgaataaaa ttttcctgaa actgtattcc ttgcatttca ttcctcatta
    11701 aaaagtgtat ttaactcact tcccgccgtt gcagcacggt gccaagataa tatttattgc
    11761 cttttaacca gacgttgcca aactcaacgg gtaatcctcg ggagaaaaag actaactgct
    11821 ccagatgcaa aacgggtgcg tcttcactga tatctaaaaa atgaccgcgt tcattaccaa
    11881 ttaatcgtgc ggcatagcga ctttccgagt aacgaatttt tctttgcgac aaactttcta
    11941 ttgttggaaa taaattgtgt tgattaaaat ctatttccgc gatgccgggg catagctcaa
    12001 tattgatacg gttctctatc agcattgctt tttcatcacc aatcgaacgt aaacgttcaa
    12061 ggtaaagaat atcctggccg ggcgttattc ttaatttctc tgccacataa cgattagccg
    12121 gttcaatccg tgacgtaata acttcagtgg taaaatgtat tttctggctt tccagcgatt
    12181 ccgcgaatga caataatcct tcacctaacg gatatgccac gttctctttt ttgacaaatg
    12241 ttcccttacc ctggacctgt attaacacgc cttcttttac cagttgggcg accgcttttt
    12301 taacggttcc ccgactcact tccagcatat ccataatctg gtattccgaa ggaatgcgat
    12361 cgtcttccac cagatctccg gtataaatac tttctcttat ccactccacc acttgttgat
    12421 agagcggtat ttgtgacgac ttacttaatg atttcatggc gcgcacctga acatttacag
    12481 ttgtctatgg acaatcatgg acaacttcaa gcgtatagta ggtgaacggg agaaaaagag
    12541 gatcgaagcg tctcacattt tgagggacca aatcgtgatc aaaaaaatgt gacgtgagtc
    12601 gatatttgtc agaagtttac tgcgccgtaa ggaaataacg gcgcgttggt aggtgttaat
    12661 cttccttcgg caagcactgc aaatgcccgt gccgcacgcc gcgctgggcg atgacgtcat
    12721 cggcaaaatg ctgcacgtca cccatgtcac ctttcagcac ggcgatttcc agacagtcgt
    12781 cgtggttgat atggacatgc agcgtggcga cggagaggtc gtggtgatga tgctgggtgg
    12841 aaacaatacg gctggctaag tcgcgttttt cgtgttcata cacatacgac agcaccgcga
    12901 aaccttgtgt gccgtgctgc tgggtagctt cctgcgccag ggcactacgc agaatatcgc
    12961 ggatagcttc ggaacggttg ttataaccac gacgctggct caggctgtcc agcgtctcca
    13021 gtaaatcgtc atcaagcgtg atggtgactc gttgcatttg cgttaaacct tttctgaggt
    13081 gcgacggcgc acggggaatg cgggtaatac cgcgttttgt agcacacgtc cggcgtcaga
    13141 ggaaaaggtt aatttatctc ccaccgcctg ggtttcgacg atttgtccgt tgtccatcac
    13201 catcactcgc tggcaaaagc gttccaccag gcgtaagtca tgagtgatga acaggcaggc
    13261 agtgccaaac tgttgttgta gctttttcag caggcgaatg acgcccgcct gtaacacgag
    13321 atcaaggtta gaaactgctt catccagaat cagcagtttc ggttcgaccg ccagcgcgcg
    13381 agccaggcag acgcgctgga gctggccgcc gcttaactga ggtgggcgct tgtcgagcac
    13441 gctgtcgtcg agatcgaccg ccttcagcat ttcgctggcg cgcgccagtt gttcggattt
    13501 tttcagtgat agcaggtggc gcatcggttc acgcaggatc tcgcgcacgg ttttgcgcgg
    13561 attcacggcg ctgatggagt cctgaaatac catctgaata tcgcggcgga acgctttacg
    13621 ctgggcgcga ttgagtttcg ccagcggttc gccacgccag ctaatatttc cctgtgaggg
    13681 cgattctaag cctaccagta gccgcgcgag agtgcttttt ccgcagccgc tgcgtcccag
    13741 cagggcgacg gtttcaccgc ttttcagggt cagggaaacg ttattcagca ccgcctggtg
    13801 ctgatgtttt ccgctaaacc caccgtgggc atagtgatgg gaaaggtcgg agacgttaag
    13861 taaagtcatg atgccagctc cataccgtag agggcgagat gagcggaaac caggctgcgc
    13921 gttaccgcat gtttgggggc gttaaacagc gtttcgacat cgccctgttc gacaatctta
    13981 ccgtcagaca tcaccgccac gtcatccgcc agacgcgcca ctacgcccat atcatgggtg
    14041 accagcagca tccccggtgc ctgtttttgc ataatgcttt ccagcagatc gaggatgcgc
    14101 gcctgtgcta ccacgtcgag gtcggtggtt ggttcatcgg cgatgataaa cggtgattcg
    14161 caaagcaccg ccatcgcaat catcatgcgc tgcaacatgc cgccgctcat ctcgaacggg
    14221 tacagcttca gcacgcgtgc ggcgttttcc agccccaccg cttctatggc agcggtaagc
    14281 gtagcgtcat cggcgggttt ccccagcgcc aggcaggttt cacgcgcgtg ggtgtgcatg
    14341 gtgcgtagcg gattaaaggc gctgcgcggg ttctgcatga tggtggcaat tttgatgccg
    14401 cgcagggcgc agggagaaac cggtttgcca tcggctaaaa tttcccccgc cgtctggcga
    14461 acgcctgcgg gcagaattcc cagcgccgcg gcgcaggtta atgatttccc gctgccgcta
    14521 ccgccgacta acgccagcac gcgcccgcgt tgcagggtta acgatacgcc gtgcaccagc
    14581 ggctgcgcgg cctgtagcgc gatatcgcgc agttcaatct gttgcggcat tagtgtgcgt
    14641 gctccgtcac cagatgaggg tccagatgat cgcgcagtgc gtcacccacc aggttaaagg
    14701 ccatcacgct gataaacagc gccagccccg gccagaacat ttgcagcggc tgggtccaga
    14761 tatactggcg cgcgtcgtta atcatcacgc cccattcggc ggtaggcgcg gtcacgccga
    14821 ggccgaggaa ggacatcccc gcgacgtgca gcatcatatg gccgatatcc agcgttgcca
    14881 gcaccagcag cgaagggatc accgcacctg ccagatgatc aacaaacact cgcacatggc
    14941 ccgcgccaga aagccgtgat gccagcacaa actcgcgctg gcgcagtgaa atcaccaggc
    15001 tgcgcaccat gcgtgcatac cacgcccagt gcgacagggc gatggcgata attacgttgg
    15061 tcagcccggt gccgagcacg ccaaccataa agaatgacag aatcgaggtc gggaaggtca
    15121 taaacatatc ggcgacgcgc atggtagcct gatcaacgcg cccgccaatc agcccggcgc
    15181 tgccgccaat aaccagccct aatgtcagca ccagcagcag gcaggtcatt accgaaccga
    15241 gcgacacgcg ggtcgctgcc atcagccgcg agaaaatatc gcgccctaag tgatcggtgc
    15301 ccaaccagtg ctgcgcatcc ggcgaaagca ggcgcgacgg caaatcaatc gcctgggggt
    15361 catacggcag ccaccactgg ctggtgagcg caatcagcgc cagcagggcg ataatgatca
    15421 gcgccaggcg taccgaccag cgggaagaga tgaaaaagtt cacgcgtgcg ctccttcatg
    15481 acgacgaatg cgcgggtcca gcgcggcgtt gagcaaatcg acaatcaaat tacagaccac
    15541 aaaaaccacc accatcatca gcgtaaagca ctggatcacc ggatagtcac ggttaaaaat
    15601 cgccgacacc gcatagcgcc cgacgcccgg ccaggcaaag atgttttcga taatcatcgt
    15661 cccgccaatc agttcgccga tgtgcatccc cacggcggtg atcatcggca gcgaggcatt
    15721 gcgcaggatg tggcgacgtt cggtctgttt gtcgttcagg ccgcgcagac gcgcccaggt
    15781 gacgtgacgc tgaccggcga cgtccagcat actggcgcgc agtagccgtg cattaatcgc
    15841 cagcgacata aaggcaatgg aaaccgcagg caaaatgatg tgctgccagc cgccgtaacc
    15901 catcgcgggt agccattgca gatacaccga aaacgccatc accagtaaaa acgccagcca
    15961 gaagttaggc atcgacacgc caagaaacgc gatgaaacgt acggcgaaat ccggcagacg
    16021 gtcacgatgg cgcgccgccc agataccgag cggtacggaa gtgagcagaa ttaataccag
    16081 cgccgcaccc gccagttcca gcgtagcagg caggaagttc agcatatcgt ccagcaccgg
    16141 gcgctgcgtg gcaaatgaga tgccgaaatc aagatgcagc gccttccaca accaggtgcc
    16201 gtactgcaca tacagcggct gatccagtcc gagcatggtg cgggtagagg ccagcatctc
    16261 tggcgtcggc ggcaggttag acagacgcaa ataatcgagc gccgggtcgc cggtaccgag
    16321 gcgcagcatc agaaaaatga tcaccgaggc ggcgagcacc atcgggatca gcagcagaaa
    16381 gcggcgtaat acgtaacgca acattaaggt ttcaccggtt taatctgttc gaacggaatt
    16441 tcggtggcga tcggcgcgta ggggatgtta cccagctccg gttttgatac caccatcatt
    16501 gagatgtaac tgataggcag ataaaccgcc tcgtcatgca gacgggtcag aatgtcgcga
    16561 tacagtgcct gacgttgtgc ttcgtcatgg gtcgccagca cttcgccgat ctctttatca
    16621 atcagcggct tgtcggctaa tccttgctgt gcctggaagt cagcgtgtga cggtacgcgc
    16681 attgaactca ggaaggcgtg tggatcatat ggcgcgcccc aggtgcggtg gaaaatcatg
    16741 ccaaaacgac cgtcgcgctg acgagcatag atactgctct cttcttcgcc aatcagcgag
    16801 acatctgcgc caatctggcg catatcagcc tgaatgattt ccgccatcga tttgcttaac
    16861 gcatcggtgc cgatgaacga cagttcaatg cgcagcggct gaccattttt ctcgcggatg
    16921 tctttgcccg caggcagcgt ccaaccggct ttttccagca acgctttcgc tttctgcgga
    16981 tcgtactggc gcggtttcag gccgaggttg gcgtagggca cagaaggggc aaacagggtg
    17041 tcggcgacct gctgggtgcc atacaacgcg ttatcaatca gcgatttttt gtttaccgcg
    17101 taattaagag cttcacgtac tgccagctca ttggtggggg ctttggcggt attaagcgcc
    17161 agcatcacgg tttcgatcgg ctgtgacagt tgggtatggt aagccgggtt ctggctaaag
    17221 cgggcgaagg tatcgagcgg taataaccct tcgtttccgt acagcaggtc gatatcgcca
    17281 gtttcaaacg ctaccgcgcg ggtcgtcggg tccgggatga cgttaaaagt gatcttctta
    17341 atcgccggct tttcgcccca gtagttttcg ttacggacga agacatcgta ctgattcagt
    17401 ttcgattcct gcaaaatcca cggtccggtg ccaatcggcg ctttaattcc gttcatggtt
    17461 tcatggtttt taaactgcga gggggcgata aagcggaaag ggcgcggcag ggccagttct
    17521 tgcaggaaag gatagtaggc gcttttcagg gtgatttgca gctctgtttt actgagtgct
    17581 ttgacatcaa caatctggtt tgccagctcc agccaggcgt gacgttgacg gttatcgagc
    17641 actgcgcgga agttttctgc cgccgcctct gcatcaaacg gttcgccgtt agagaatttc
    17701 acgtcatcac gcagggtgaa ggtccaggtt ttaccatctt ctgaatgagt ccagcttttt
    17761 gccagccacg ggatcaccga accgtctgcc tgatatttca ccaatggttc ataaaccatg
    17821 ctctgggcga acatctggtt aggcgtgtaa aggtgcgggt ttagtggccc gacattcacc
    17881 ggccaggcgg tggtgatttc atctggtgca gcggcatgga cgataaaaga cgcacaagcc
    17941 agcagcgcaa atagagtgcg gcggagtgtg gagagcatgg taaccccaat ggattaaaat
    18001 agatggcgga aataagtatg acgattttaa gtattcgtca tactgattac ctgttctgga
    18061 tcaatagatg ggcagtcggg tgggcggatc agttaactga atcgatccat tgcacactgt
    18121 cggcggtgag cgtaaagggg gtgggggtgc agatcgcaag gcttaaattt tctagctgac
    18181 aatggctgac tgacagcgag gagtgatagg tgctatctac gctgacgatt tgccaggcgc
    18241 tgccaccacg ctgtttaacg atggcttctt tgcgcgtcca gatgcgccag aacatttcca
    18301 gttgctgatc aggatgcacg gcgtccatct cagcgtgttc cccgaggctg aacacggcgt
    18361 tcgccagcca gcgccagttg gcgcgcgggc gaatcacttc gatatcgcag ccgacttcgc
    18421 cttcatcact caacagcagg gcgatatcgt caccgctatg gcttaagttg aaccatagcg
    18481 gcgtttccgg cgcaaatgca ggtttgcctt gttcgccata gatgatctcc ggtagcgggg
    18541 aaagcgtgtg cgaaagcaat gcacgccccg ccagccagcg ttcgcgtcgt ggaccttgcg
    18601 gtgcttgctc gcgtaaaccc ggtggcagtg gagctgcgct taaggtcgaa actttcccca
    18661 gaactatccg atacatatca gggccaacgt ttaatgatga tggatgtatt aatgccgcca
    18721 aaggcaaagt tgttactctg caaaaactca caatcaacct tgcgggcttc atgcatgatg
    18781 taatccagcg cgccacaatt cgggtctggt ttattcaggt ttagcgtagg cgcaaaccaa
    18841 ccttcgcgca tcatttgcag actcatccag gcttcgagcg cgccacaggc accaagcgta
    18901 tgaccaaaat aacttttcag cgaggagagc ggcacattat caccgtaaat ggttgctgtc
    18961 gccagacttt ctgccatatc accgcgatcg gtcgccgtac catgcgctga aatatagccg
    19021 atatcttgtg cgcttaaccc tgcgattttc agcgattgct ccatacaata ctgcatggtt
    19081 tcgcgctgcg gctgggtaat atgtgccgcg tcgcagttag tcgcaaagcc gacgatttca
    19141 ccgtaaatgg tcgcgccgcg cgctttggcg tgctccagtt cttcgagaat gagcgtacct
    19201 gcgccttcac caatcaccag cccgtcgcgg ttttcatcga acggtgaagg tgtggttttc
    19261 ggcgcatcat tgtgctggct ggtggcgaaa agcgtatcaa ataccgctgc ttctgaagga
    19321 cataactctt cggcaccacc agcaaccatc accgtttgat aaccgtggcg aatggcttcc
    19381 cacgcataac cgattgcctg gctgccggaa gtacaggcgc tggaggttgg gatcacccgc
    19441 ccgcgcaggc cgaaaaagag gccagtatta acggcggtgg tgtgcggcat catctgcaca
    19501 taggtggtgc cagtgatgtt attggtgtgc ttttccgtca gcatggtggc gaactcgctt
    19561 accgggccgg tactgccggt cgatgaaccg tacgcaatgc ccgtttcgcc gctggttaat
    19621 atcggatcgc caatcaaccc tgcctgctct aacgccagct cgctggcacg ggtcgacatt
    19681 tgcgacacgc ggcccatcgc acggatacgc ttgcgggtgt agtgctccgg tagcgtgaaa
    19741 tcatcaacag gcgcgccaag cagggtgtgc agcccgtcgt agacctgcca ctccggcatt
    19801 ttgcgcaccg cattttcata cgccagtaag cgtgcagaaa cgtcctgcca gttttcacca
    19861 aaggcagtca cgccgcccat tcctgtaatc actacgcgac gtgtcataac atccctccgt
    19921 tgatggaaat aacctggcgg gtgacgtaac ccgcaatatc agacattaaa taactggcaa
    19981 gcccggcaac ttcttccgcc tggcccatac gtttcatggg gatcatcgac attgcctctt
    20041 tcagagcgct ctcttccatt tcgatcatcc cggtatcaat aagccccggc gcgatgcagt
    20101 tgacggtgat tttgcgcttc gcgagttcga tagccagcgc ttttgttgcg ccgataattc
    20161 cggctttggc agcgctgtaa ttcacctggc cgcgattgcc cattacgccg gaaaccgagg
    20221 atagcgtgat gatgcgccca ccctggcgtg cgccgatcat cggcataatg cacggctgaa
    20281 tgacgttgta gaaactgtcg aggttggtgt ggatcaccgc atcccagtcg tcatcgctta
    20341 aagccggaaa agcggcatcg cgggcgatcc cggcgttact gaccacgccg taccaggcac
    20401 cgtgttgcgc gatctcatgc tccagcactt cccgacactg ttcgcggttt gcgacgtcga
    20461 aactgagcaa acgcccgtta cccccattgg cgacaatggc attcagcgtt tcctgagcgc
    20521 ctgtggcgtc acggtgatag tgaacgccaa tgttaaagcc gtctgccgcc agttggcagg
    20581 cgatggcgcg accaatgcct ttgctggcac cagtaaccag aactgaacga ctcatcagga
    20641 cgctccttgt tgaaacaggg tggtaagttc ttccgcggtc ggctggaagg tgttaatgcg
    20701 gcccgtcgcc tcgccgtcgt tgatgctgca ctcaaaactg ccgaagcgat cgtcttgcat
    20761 cagcagtttg acggtaatgg tcagcgtctg gcctgcgggc agaatgccag tggtgcaaag
    20821 caattcacgc gcgcccagta tcatccctaa ctcaatactg tttttgccct gctggtggcg
    20881 atgccagcca gaccagacgc cgacggtttg cgccatcagt tcaagggtaa accagccggg
    20941 gagattgccg tccgggtcga gaaacggtgc cagtacgcca ctgggcgaaa ccgtcacgcg
    21001 gcacaccgcg ctgtcatcgc tgacgctcac cacgtcttcc agcagtaaca tgggcgcgtc
    21061 gtggggcaga taagcgccgg gggataaata gtggctcacg aaaccctccc gagcagaatg
    21121 ctggcgttat tgccgccaaa ggcgaaggaa ttggacagga tcaccggacg tgccagcggc
    21181 tgcggttttt cgataatgcc acacggtggc agcgtgggat cgcgcggcga caggctgaag
    21241 tcctgagcgg gcagcggtaa atcacgctgt aaaatcaaca tactaattgc cgcctcggta
    21301 atgcccgctg cgcccagcgt gtgtccggtc aaatgtttgg tggagctgca cggtacgcgt
    21361 tcgccaaaca gggcgttaac caccattgat tcaatctgat cgttgagttg tgtggcggtg
    21421 ccgtgcaggt tgatatagcc gacgtcatct ggcgtaagct gcgcatcggt cagcgcctga
    21481 ttaatagcgc ggatcgctcc ttcgccctgc ggatgcgggg ctgaaatatg gtaagcgtcg
    21541 ctcgattcac caacgcccag caacgcgatg ggctgcggtt cccgggtcag cagcattagc
    21601 cccgcccctt caccaatggt gatacctgcg cggtcacgac caaacggctg acataaggtt
    21661 ggcgacagcg attcgaggct gtgaaaaccg ttaatcggca tccggctcag ggtgtctgcg
    21721 ccacccacaa tggcggcatc gaccagcccc gcttcgataa gcctgcgccc gctgattatc
    21781 gctctggcgc tggaagagca ggcggttgaa agggtatagg ccgggccgtc gagcgccagc
    21841 cagtggctga gaaaacgcga cggatcgccg agttcctgtt gcgcgtagtg ccatgtggtg
    21901 ctcggctgcc cgtgggtgcg caggttaacg tgcgtgtcgc cttcatgcag gccggaggtg
    21961 ctggtgccaa gtacaatcgc gatgcgctcg cgaccatatt tcgctatagc gtcatcaacc
    22021 tgcggctgga gctgcgccag cgccgccagc aggatctgat tgttgcgcga gcgatgcgca
    22081 gcgaattttt ccgggataag cggaagctcg ccatccacgc cagccaggac cgcctgcgga
    22141 tgcccctgca accagcctgc gcgtgggcgc attccggggg cgacaccgcg agccaggttg
    22201 gctgagattt cgtcaaggtt atttcccagg gcgttgatca tgccgacggc ggaaatataa
    22261 atcatatcag tcacctaagt attgaatggt gatgtggtat ttaaagacat gctgctcaat
    22321 gctgattggc acgcgtttgc ccttgcgatt cagataggtg atttccgtga ccagtttgcc
    22381 gctggcgtta cgcagctcgc gtttgtcgcc gttgtcgcga agcgtccagc cagtgggaag
    22441 ttgcggttgc cagacgctaa tcggccagtg gctgagcatc acgtcagcca gcacctgact
    22501 tgcgggcggc agctgcggga cgacgatgga ttgctcggcg cgtagccctt gcgcatcgta
    22561 ggtcaccaga aacaggcgaa tgccgaccga tgatagtccg gcaagggtga ttttctggtc
    22621 gtcggcgtta agcatcacta gcagcgactg ggttttgccg ttgaagctgc cggtcaacag
    22681 ttgctgggaa ttgacggcgg gtgaaatccc cggcgcaggc agcgtgatac gcgtgccagg
    22741 ttgcagccat gcctgcgggc ggccttcctg ttccggttgc gagtgactac agccggtgag
    22801 gataagcgta gcggtcaggg cgaacgctcg ccagaatgtg ggtttgatca tttttttgtt
    22861 ctctttttat cgggcatagc cagcggcgaa agcaggaagg cggtgaaaat accgctcacc
    22921 agcacaatgc caaaactgct gatggcctgg gtggcgctga ataccagcat tcccagcgtc
    22981 agcaaggtgg tgagcattgc cagcgcgatc gccagtagcg aagttaacgg tgtaccgcgc
    23041 ggattactga aaaacagcgt gtagttgata ccgatgccaa gcaccagcac cagcgccagc
    23101 agcgaaaaga gattcaccgc ctgcccgctc atcgccagca ccgccagacc acagcccagc
    23161 gaaagcaccg aaggcaccag gctgataagc cctttgcgcc agccgagacg ggccactgcg
    23221 ccgcaggcaa tcactgccag cgccaccagc aacaagccgg ttaagacgta gcggtaaagt
    23281 gcgaacaatt catcaaaggt gcttttgcga tcaacccagg caatgccgca agggtaatat
    23341 gtggcgattt cctgcatcaa cgcgctactt ttaacccctt caaccggcac cagtacgccg
    23401 ctttcgccat tttccagcgt cagccacagc agacgccagc cttcactggc ggggcttgcc
    23461 agccattcat caacgttcac tggcatggcg ttgagatccg ggttcactgc cgtcagcccg
    23521 gcattttgca gcgcttttgt tactgtcggg gccgccgttt tcagcagttg taaatcttcc
    23581 tcctgccgcg caagggagtt cagcggaatg gtgcggtagt tgctgataag cccctctttt
    23641 ttcgcatact caagtgaggc ggtatatttc tccagtcgcc gcaatgtttg ctgtggcgaa
    23701 tcgccgtaaa ccacaaacca tttttgatcg acgctttgtc cggtcagggc ggtaatggct
    23761 ttttcctgcg ccagaatgtg ctgcggcagc gcctgtaact gcgagatatc gtcatcgacg
    23821 cgcagcattg acatccccgc cagcgaaaac agcgccagcg cgacgggcag acccagcgac
    23881 agttttttat tgcgccgcca cgctgccagc cagcgcagca tcagcgccat cgccggaacc
    23941 gggcgcaccg gcaggccacg gcacagccac ggatgccaga acaggacggt cagacaagag
    24001 gcgctcaacc cgacggcggc aaaaatcgcc atctggtgaa ttccggggaa gggggcgagc
    24061 atcataatca gatatgccgc cacggtggtg agcagcgcca gtagcaaggc attgcgcact
    24121 ttcgccaggc tttgccacgg cgaaacgtcg ttgccgtgca ccatccgctc ggtgagataa
    24181 tagagcgtgt agtcagcgga aatgccgata acgctcatgc tcatcaccag cgtcatcagg
    24241 tgtaattcac cgaaaatcaa taaagtggcg accgttcccg ccagcgcgcc gatgccgatg
    24301 gaaatcacgc aaagcagcaa cgggcgtaaa gagcggaaca ccgccacaat cagcaatatc
    24361 acccccagca gcgtagccac tcccagggtg gagatatcct gcttcgcctg ttggctggcg
    24421 taatcgctgt aaaacaccgt gccgcgcgag agcaactgtg cctgcgggta acgcgttttc
    24481 agatcctttt ccagcgtatt cagggtcgtg atgagctggt gggtttgctg catatcaaac
    24541 gacgatcccg ccagttcgcc gtgcagcaga taccagtagt tgccctgggg atcctgcgtc
    24601 accagccaac cgtccatcag ccgcaaacgc tggccgtttt tcgccattgc cagctgcgag
    24661 ccgcgtatta acatcagcgg atcgttttgc agctccttgc cgcttacccc ggagaatgcg
    24721 gaataaagct gggagaggat ccactgtgcc tgcgcttcgc cgccgttttg caggcgggcg
    24781 cgggtgttgg ggtcaatcag gccgttgcga tgctgccaga aaaacgctcc ccacgcctgc
    24841 tggctggcgg catccattgg ccctttaacg tcgccgagcg cagcggattt ttgcagcagc
    24901 gtcagccact cctgagcgac caaaggatta gcctctttac cggggctgac cagccacacc
    24961 agttggcggt caagacgctg cataaagcca tcattcagcg ctgggggaat atcgcccatc
    25021 gcctgtttgg gtaacatagc cagcacgcta ctgttcagcc gtgattgcgg cagcaggatc
    25081 agcaacgccg ccgccatgac caggcagact agcccccata acagcgcggg gcgtttactg
    25141 ggcggcaaaa cgttggcgtt cgtcatcggt cagttgcgct ggcgtcagtt gatgttgggt
    25201 aagagcaata tcggtgcgat cgccctgttt atcattaagt tgaatgctct ccagataggt
    25261 tttcccggcg agatcgatgg tgttgaaaat tttatccagc ggcgtggtgg tcggcgtcag
    25321 gcgcagcgtc cagcggcctt cgcctttgtc agcaaattcg acgcggaagt tttgttccag
    25381 cactttgcga tcggcctgga acagcgcgcg cagcaggtgg ttaaactgga acatctgcgg
    25441 gttgttttct gccgtgatga tttgcggcgg ctgaccgttg atcacctgca ccatgcgttt
    25501 atcgtccagt aatagttgca tggggaacgg tgaggtttga tcccacaaca gcccctggtc
    25561 gcgggcgatc aacatctggc cctgagatcg cagcggctgc ggcagatctt taatcgtccg
    25621 ggtttgatcg aaatgggcgc ggatcaccgg ttgttcggta aagcgttgct gaagatcgtc
    25681 cagggtcagg gcgctcacaa acgggctaat cagcagcgcc agcagcggta aaaatttcat
    25741 ggtttgactc ccatgcgttc aaacagaata tcggggctga caaagcacag ttcgcggctt
    25801 tgctcgtcga ccgcgacctg aatggtgtat ccggtggtgg cgcgtttgcc cgtttcggca
    25861 tcgaaaatct gatagccaat ccgcagacgg ttttcgaact cttcgatatg cgcacgcacg
    25921 cggatgcgtt gctcaaaggt gagagcgtgg cgatatttca cccgcgcatc gaccactggc
    25981 cacaaataac cggactcttt catctgccga tagccgtaat tgaactggtt gagcagcgcc
    26041 tcgcgggcca cttcaaagta gcgaaaatag ttaccgtgcc aggccacgcc catcatatcg
    26101 acgtcgtgaa agggaatggt cagctcgact tcagcggtaa atcggggatc gttaagcacc
    26161 ctttactcct tgtcctgaat ttccggcagt tgccagaaat cgaaaaaatt aaaccagtcg
    26221 aggggcgact ggagcgcgta atgttccaga cgcgcggcgt agtggtcgat agcgttttgc
    26281 agcgcctgtt ggcgttcgcc acgcggcaac agcagcgggt cggcaaaggt ttcgcagtga
    26341 atatgcagct tgccatgctg gcgtagggcg aaaatcagat tcactgggca gcgcaaaata
    26401 gaggcgagaa taaacggccc ctgtgggaag ggcgcaggct ggcccataaa cggactccag
    26461 cagacgcgcc agtcgccgcc gcgttgcggg gtgacggcga tgcggtcacc gacaatcgcc
    26521 acccattcgc cgttatccag cttctctttc agcaggatgg cggtttccgg gccgatatct
    26581 gttaccggca tcaggttaat gcctgcctga ggagccatct cctgcattat ctgtttaaag
    26641 cgttgggcgt tttcgctaaa caccagcgcg ttaatggttt tgtagccctg aatttttgcc
    26701 agcgcccggc acacttccac atcgccaaga tgcgaggcca gcagcaattt gccgcgcgga
    26761 tcgcggacgt caagcgccgc ttctgcgcct ggcgcaaaca gcacatcacg ccccagttgt
    26821 agctcgccgc gccagctggc gattttgtcg agcatggcat taccgaaacg tagaaagtgc
    26881 tgatagctgt tgagttttgc cgcctgtggt tgatgctgtc gtacacgggc gagccagtct
    26941 tgcgacgctt tgcgcgcacg agaagcagtg agccagtaga cgcccaccac cgggtaaagc
    27001 agcgcgctaa acgccgttct tcccagcaga cgccagacca gcagcatcag gcgcattccc
    27061 cataatcctt tcacttcact ctgccgcgcc cagtggctgg aagagcggcg catcagcagt
    27121 gaaggaatac gcggcaacat gccgaaaaac agacgcgtgt gcatgagcga gatgcggacg
    27181 ttatctttca gggcatcaaa atgcgaaagc ccgtccagtg gataggtgac gcgggtcggc
    27241 acgaaatagc tggtgtttcc ctgccagtag aggcgcacca tcacttcagt gtcgaaatcc
    27301 atccgcttgc cgatggtggc gtgttttgcc agttgcagcg ttggcgcaac cggataaacg
    27361 cgaaagccgc acatgctgtc tttcagttgc agggagagcg tttcgatcca tacccagacg
    27421 tgggtgaccc agcgcccgta aagccgtgag cgggggatgg agtcatcgta aattggctgg
    27481 ccggagatca gcgccgcagg gtgttgttca gcgactgcca gcagtttggg gatatcttca
    27541 atcgcatgtt gaccgtcagc atccacctgc accgcatggc tgaaccctgc gtctgccgct
    27601 gcctgtaagc cacgcatcac tgccgcgcct ttacctgcgt tttctgctaa gcggattaag
    27661 gtcacgccag gctgtttgct aaccagatta tccagttgct gttgcgtggc ggcatcgctg
    27721 ccgtcgtcca ccacaataca ggggagatta aatggcttaa gacgcgccag cacgcccggc
    27781 atcatcgcgc cgtggttata gcaggggatc aacacgcagg gagaaaagtt taccgacata
    27841 ggcggatctt cccgctactg gcggtgtggc gggcgtcacc gtcgtgacgc tgatagctaa
    27901 agctcagaat ctggcgttct tcctgccagt taagcgcgag cgtgacgcgg ttttccggca
    27961 gcaacggaga ctggaatttt atgttctgaa tggagtgaaa acgccagcca ggcgcgagca
    28021 gattgagcgc gtagctcatc gcccagtcga tttgcgcaac tcctgggagt aatggttgca
    28081 cggcaaaatg accgcgaaac cagaacagat ccgccctgag atgcagaaca atttcaaggt
    28141 gattcgtttg tgcctgatgg cgctcgattt catggggctt catgaaataa ctcctgtaat
    28201 tgcgcataga cacgcttgtt catactgttt actggcattt catcaataac gcgccagtaa
    28261 cgcggaatag caactggctc cagcgtcggg cgcagtaaac gccgccaggt catttcctgg
    28321 ctatgcccgc cgccgttctg ccattgcaga tgagcttcgt catccagcac cagcaaaacg
    28381 cctatgctct gacgcccgcc gcgcgtaacg ggaactgccg ccgcctcgcg aatgccgtcc
    28441 agcgccagta gacgttgttc tacttcctgg agtgaaatac gtttttcttc aatcttaacg
    28501 atgcgcccac gacgccccat cagatggaac tgaccatctt cgctaaactg taagatatcg
    28561 tcgagtaaca ggccgttatc ttccgctatc agcggtgaaa agagacgaaa agaatcacct
    28621 tcggcctgga actgcatacc agggaagggt agccagcggc gttgctcttg ttcgcggtaa
    28681 cgccaggcga tgacaccggt ttccgtgctg ccgtagattt catcaggcca gacgcgcagc
    28741 cagcttgcgg tgtgttgtac atcctgccac ggcagctcac cgccagcaga gagaagcacc
    28801 tgaacggggg gcggagaaag ctgggtatcc aggcgcttca aaaatgccgg gctgctgatg
    28861 aagatatagc gatgctgatg actcaacgcg gcaagctgtt cgacatacca gagcatggcg
    28921 gcatgcagcg gtaatcccaa cgccatgggc aggaaaacgc gaaaagtcag gccgtacaaa
    28981 tgctgcggca atacggaacc gacgacacga catccggcaa gacgtgcgcc taatcgttct
    29041 gccagcagtt ctgcttcacg atccagcaaa tgaacaggtt taatcacccg tttcggctgt
    29101 cccgtggaac cggaggtaaa cagctcgata aaagcctctg gcgctatagc ggaaaaggtg
    29161 aaggattgtg tcgcgatttg tggagaattt gctaccaaca gcaatgaacc ttgccagtta
    29221 agttcgctgt cgctaagcac accatcaaaa agttcacgtt gctcgttaag ctggataaca
    29281 cggttatgcc ccggaagcac cggcgttttt ccggcgtgta gcgtggcgag cagggcaacg
    29341 ataaacagat agctgttctc aaagcacagc gcccagcgtt cacccggctg ttgctgcaag
    29401 cggcagataa gttgcgcgac atcgtgacgc aaatcgccca gcgtccagaa gctttcgtcc
    29461 agccacgcaa tgggtgtgtc atcagggcgt ggcgcagtca gccagcgtgc aagcggtaag
    29521 gtctgcttca tgcgcgatct cttttcataa cctggcggcg caccagccat tcaccagcca
    29581 ttaacgtccc catcaacaaa taagcaatca tgccgttcca ggcggtccat aacgacatat
    29641 cggcataaag cgcggtaaag agggcaattc caccgttaat gatgaaaaag ccgcaccaga
    29701 tttgcgtgac gtggcgggta tagcgcacgc ccttttccgg gaggtctggc tcctgcaacc
    29761 gcgccagccg ctcaactatc ggcattgccg accacagcga accgccaaaa accgccagca
    29821 tgaccgcgtt aaccactacc gggtaaaaca gcagtagctg gtgcgttttc aacataaagc
    29881 tggcgacaca caatgcgatg cccacgacag cgacgagttg cgtgacggct tgtaaaggcc
    29941 ccgcctggcg acgggtctgg cgcagacgca acaaaagcag caacgccatt agcggtaaca
    30001 gccagtgcag gccgttatgc gctaagccaa accaaatcac aaacggccag gcgagcagca
    30061 ataatccagt cagcagctta atcatcggta gcgagcgaat gcccgacatc agatcaggct
    30121 tcttgtagca ggcgttctac agcctcgacg acatcctgga ctgtacgcac tgctttaaac
    30181 tcttccggct tgattttctt accggttttc ttttgcaggt gcacaatcat gtcaacggcg
    30241 tcgatgctgt ccagttccag atcttcgtac aggcgcgctt caggtttgat gtcctgcgga
    30301 tcgatttcaa acagcttaac cagcagggct gagacttcct gatagacggt ttgttgttct
    30361 gtcatgatgg ctttcctcag gcacgttgcg cagcaatgaa agaggccaga gtggcgacgg
    30421 agaaaaagtg ctgacgcatc tcttcgcttt ccgcagagag cacgatgccg tactcatttt
    30481 ttactgccag tcccagttcc agggcatcga tagagtccag ccctaaaccg tcgccaaaca
    30541 gcggcgcgtc ggtatcgata tcatcaggcg tcaactcgtc cagattgagg gtgctgatga
    30601 tgaggttttt aatttccaga taaagcgctt gcattattta ttcctgagag aggtaaacaa
    30661 ccttgttgta attgaagcaa aagatgccgg tttaactgcc ttgctgccag cgccggttct
    30721 tgtgaagttg catcgtaaaa tttattgatt tcaacccgac catggactgc aaccgtaaaa
    30781 aacggccggt caggtggaac gtcataccat ttactttgtt tatccagcag atgctcgcta
    30841 cagccgatcg tcacgatgcg taaatcgctg gcacagcgca cggcaatatt cgccgccccg
    30901 cgctgcagcg tcattttttc accgggcttc gttcgcgtgc cttcggggaa aatcaggata
    30961 gtgtcaccct gtgccagtcg ctgttggcag cggggtaaca gggtttcagc ttcgctgttg
    31021 attaaataat ctgccgcccg cacgacgccg ccaagaaaag gattttttaa cagcgcgctt
    31081 ttcaccaggc agtcagtttc tggcatgact gacgccagca gcacgtaatc gatcagcgtg
    31141 ggatgattgg ctacgacgag gcaaccgcgt tcctgacgta aaatttcagc gccatcaata
    31201 cgataatcca gcacgccaag ccctttcgcg acagttaaaa acaggcgaaa actggcggca
    31261 atgctgcgac gcgccaaacg acggcggcgg gaagtatccc acaccaaaac taacaatatg
    31321 ttaaaccaca cgacagaaag cagcaggccg ccgagaccaa acagggcaaa acataacccg
    31381 gtcatgacca gtcgccaggt ccactccaga cgtgacagca gcttactcat ccacggctcc
    31441 agcgccactg tacgcgctcg ccaggcagtg aaaatgtgtc cgcattttgt aaatagtgct
    31501 gtaaaaacaa catgctttgc ggcagtgttg tttcattacc cgcaatggct gactgcgttt
    31561 cacattgcca ctcatcgcca gcttcaatca ccagcgcgac ggcgtacggc caggtcggca
    31621 tttctgcggg cagttgtgga tgatagaact caggcagaaa gccatcaaaa tcgaccatca
    31681 gcacgcgctg gtatcccgct tgtaacaggc taagcacttc acacaaacct tgctggaaag
    31741 tgtcgcgacc agcagaaagc gatgacgaaa cgatcggctg tttggccgca atggtcaggt
    31801 tacccacgga agagttgtgt actgaaagtg caaagtcggt cggagaaagc gcctgttcgg
    31861 ttgccagtgc atgaacaatg cggtaattgc gctccagttc gccatgacga ctggtataca
    31921 aaacggcgtc tggctgatgg cggcgtagca ttgccagacc gcattcaaca gccagtttgc
    31981 tgcctgaact gaggcggcgg gcggtcatca ttggcagttc acttaatttt gccaacggcg
    32041 cggcagggtc aatcgcccac ggctggcgcg accatgcttg ccattgctgc gcttcgctaa
    32101 gtccaggcgc cagtgcctgc cagtcggtaa tatttaatgt aaatttcatc aacgcgcgcc
    32161 cgcggtaaac caaaattatt aagtggcgat tatttctttt ggcatatcaa aagtgtatgc
    32221 ccaacgccta aattatcgtg gcgctctgct acctggaatc ctgctttatt cagatagtcg
    32281 taaaatttct ctgcactata gaaacggctg ttaccgtttg ccatgcaggt aaaataaagt
    32341 gaggacgcgt tcaggctaaa cgatgctgct tcaaaccgct gcgcatccca gaacaactcc
    32401 atgatgcaaa cttttgcgcc aggcttcatt acgctggcaa ttttgctgag tatgctaatt
    32461 atttgttcgg gtgaaaaaca gtctaaaaat tggctcatcc accagatatc agcctcgccc
    32521 ggtaatgggg catcgcttag catatccacc gcatggaaat ctatgcgatc agaaaaacct
    32581 gcattcgcga tattttcttt cgctagtacg atttgttgtg gcagatctaa taacgtcacg
    32641 gcgatgtttt cattgtattt gcagcaacgc aaagcccact tacccgtatt gccgcctaca
    32701 tcgtacaatg ttgtggggtt gttggcgaat acataaggca gtgcggcgtt aaatgcgcca
    32761 tctgaatagt aatgatcaaa cgcaaaccag ctttcacgag cggcgtctgg taattgcgaa
    32821 agtgccggat atatcgtcga ccagtcgcca aatactttta acccggaagg ttttccttca
    32881 ttgagtgaat cggcgaggaa gaagagcccc tggtaacaga catcctgggt gaaatccata
    32941 ttcacgcgag tcatagtgtc atgcaggagg taatgaccga ttttcgccag gtaataaatt
    33001 ccctctttac aggtaataat tcgcccgctt aagcccatat ctaacagaac gctggcagca
    33061 tattcgttga tatggctatg ttcggtgata tcagccagcg ttgcgccatg tttgccttgc
    33121 cgatccagat atgaaagtac acccgcatta cgtaggcaga gtgctgtttg aaaaagcata
    33181 ggggcaaagg caatgcgttg agcttcggta atcgcatcca gtgcgctaag agaatcctgt
    33241 tcgtacatgt tgggcacaac ctgttaagag ggaattaaat tattatcgga ccgcaacggc
    33301 ccgactttag gaagaattat aaatctgcgc ccgctgacaa attcagttga atatctttgt
    33361 cgagattgcg gacccagcga ttgtagtttt tatgaatttg tccgcctgac gcttgtaggt
    33421 tctggctatt ttcataattg atatcgtagc tggatgccgt gtagttaatg cgtatttccg
    33481 ctgaatgatc gcgtgactgt tgacgtgctt taattatgcc ttgtccggct tcggacataa
    33541 tccatttgcg ttcaacgcca gctttcagaa tcgctttttt cacttgttcc tgggtatgac
    33601 cagcgctgac ggttgagtga acctgatcaa ttggcgcagt acgcgcgcaa cccgctaaag
    33661 cacccacaac agcaatagcc gcaaaccatt tcatgtaatg tctcattaga accccattaa
    33721 agtattgttt ttacgagtaa agcaagtttt aactgtaatt gctggcatca ctattcttta
    33781 tttagagaaa aaattacttg tgagataagt cagtaagtgc acatcagagt aaaaatctct
    33841 gaaatgacta aattactgcc agcagggacc aactataagt gagtgagaaa aatgaggcaa
    33901 taaaaataac acaatgttat cagtgcattc tttgagaagg atttaatcgc tattcggcgt
    33961 ttatatatat ttgacttaag gaatggtttt tttaaattaa ttttctgaat aatgtttcat
    34021 catttcctca aatcaaatgg ccactgttag ataaacggtg gccattgcct gctcaggagg
    34081 tgctattaac ccggtaaccg acttttcggt ctgcccggcc ccagtaaaat cgccagtttg
    34141 ctgccgcctt tcgtcgtttc catccagatt ttacacacgc tggttaatgg caccgaaagt
    34201 agcatcccta ccgggccgag cagccatccc caaattaaca atgaaagaaa taccaccatg
    34261 gtggacatcc ccaggcgatg gcccatcatc cgtggttcta aaatattgcc gatgaccata
    34321 tggaccacta aaaacaatgc gccgaccaga atacattcgt aaacaccatt aaacagcagc
    34381 acctgaatca ttggcggtac ggcggaaatt accgcgccga tattgggcac gtagttaagc
    34441 aaaaacgcca gtactgccca catcagcgca aactgcaccc ccatcagctc cagccccagc
    34501 cagacgatga cacctgtcca taaactgagt agcgtcttta acgccagata atgcgaaacg
    34561 cctttaagtg cgcggtgtaa tcctgcgatg tgaatctgtg ggttattcag cgcaaaacgc
    34621 attttgtaag ggacgtggcg cacttcaaac agcataaaaa ctacggtcat caccagcaaa
    34681 agcacgctcg ccattgcccc ggaaagcccg gtcattagcg ctgtggtgaa ggtaaccact
    34741 ttttccgagt ccatccgctg cagcattcgc tccggcgaca tatgcaaatt aagaaaaggc
    34801 aacatctcct gcaatttaaa aagtttgcgc gtcagctcct tattaaactt cggcagcata
    34861 gagataaatt cgttaaacga tgccgccagt acgccgacca gcgcggttag tgcgatcagc
    34921 atcaccacca ctacaatcgt aatggcaacg gggcgttgta ctccccgacg aataaaccag
    34981 gtgacgagcg ggttgaggac gatggcaaaa aacagcgcca gtaacagctg cacaatgata
    35041 tctgccgctg cgtgaatgcc cgcgaggatc actaccagcg aggccagctt gagcagaatg
    35101 tgcatgcccg ttttatcggg ttgaggggtt tccattgggg cttccttgtg actttttgta
    35161 ttaagtgtag cgggagtcac gccagcaata ttctgattct tactgctgat tttcacgtca
    35221 gcacgtggta aaaatgaaac actgttgtaa aaatgtggtg atcctcatgc ccgaacccgt
    35281 agccgaaccc gcgctaaacg gattgcgcct gaatttgcgc attgtctcca ttgtcatgtt
    35341 taacttcgcc agctacctca ccatcgggtt gccgctcgct gtattaccgg gctatgtcca
    35401 tgatgtgatg ggctttagcg ctttctgggc aggattggtt atcagcctgc aatattttgc
    35461 caccttgctg agccgtcctc atgccggacg ttacgccgat ttgctgggcc ccaaaaagat
    35521 tgtcgtcttc ggtttatgcg gctgcttttt gagcggtctg ggatatctga cggcaggatt
    35581 aaccgccagt ctgcccgtca tcagcctgtt attactttgc ctgggacgcg tcatccttgg
    35641 gattgggcaa agttttgccg gaacgggatc gaccctgtgg ggcgttggcg tggttggctc
    35701 gctgcatatc ggacgggtga tttcgtggaa cggtattgtc acttatgggg cgatggcgat
    35761 gggtgcgccg ttaggtgtcg tgttttatca ctggggcggc ttgcaggcgt tagcgttaat
    35821 cattatggcc gtggcgctgg tggccatttt gttggcgatc ccgcgtccga cggtaaaagc
    35881 cagtaaaggc aaaccgctgc cgtttcgcgc ggtgcttggg cgcgtctggc tgtacggtat
    35941 ggcgctggcg ttggcttccg ccggatttgg cgtcatcgcc acctttatca cgctgtttta
    36001 tgacgctaaa ggttgggacg gtgcggcttt cgcgctgacg ctgtttagct gtgcgtttgt
    36061 cggtacgcgt ttgttattcc ctaacggcat taaccgtatc ggcggcttaa acgtagcgat
    36121 gatttgcttt agcgttgaga taatcggcct gctactggtt ggcgtggcga ctatgccgtg
    36181 gatggcgaaa atcggcgtct tactggcggg ggccgggttt tcgctggtgt tcccggcatt
    36241 gggtgtagtg gcggtaaaag cggttccgca gcaaaatcag ggggcggcgc tggcaactta
    36301 caccgtattt atggatttat cgcttggcgt gactggacca ctggctgggc tggtgatgag
    36361 ctgggcgggc gtaccggtga tttatctggc ggcggcggga ctggtcgcaa tcgcgttatt
    36421 actgacgtgg cgattaaaaa aacggcctcc ggaacacgtc cctgaggccg cctcatcatc
    36481 ttaaaactta ctgaataacc agcgtattaa tgatgttttc tgcggtggtc tgcgcttttt
    36541 gctgatcgtc agctggcaga gtaatttgca tggtcagcag ttgattaccc acgttaccca
    36601 gaataacgga agagtacgcc gtctggcctt tcgcggagat aatactgtct aactgctgca
    36661 ttttgtgacc tttcagctca atggctttat tggttaccac ttgcagctgc ggatcgcggc
    36721 tacgttgctg atcttccaga cgcttcgcca gcaccgccag atcttctttc ggatcatcgc
    36781 ccatgatgac gatgactgct ttctgcccgg tggcgtcgga ccagacatgc atgttattgg
    36841 cctgcgttcc cagcttaccg ctctggtcgg tcatatccgc tggcagcgag aaacttaact
    36901 tgccatcaag caggttgacg ggattcccgg tagcgttact ttccgcgacc gaaccctgcg
    36961 ccgtagcgtt agtgtcttta tcatcacagg ccgcaagccc cataaccagc aggccaattc
    37021 cgacatattt aaccagattg cgcattgact tcttcctttc gataaacggc cataacggct
    37081 cattcatcca tcttatcaca actctgataa cgaaccttta actcgcctgc aaagcgttga
    37141 tttcggattt atctgccagt cttttcaaca gcatattgag taatacgcca tacattggca
    37201 ggaagaaaac gatactgatt aacactttga aacagtaatc gaccagcgcg atttccatcc
    37261 agtgttcagc cataaaggca tccgggctac gccagaaggc aatgaagaaa aaggccagcg
    37321 tgtcgctgac gttaccgaac agtgtggacg ctgtcggtgc cagccaccag cggcgactct
    37381 gacgcaggcg gttaaaaacg tgcacgtcga ggatctgtcc cagcgcgtag gccatgaaac
    37441 tggcggtggc gatacgggcg acaaacaggt tgaagtgggc gagtgcgccg aatccctgcc
    37501 aggaacccat atagaatagc gacgagatga cgtaggagat cagcaatgcc gggatcatca
    37561 ccgcaaagat gatacgacgg gccagcggtg cgccaaaaat acgtacggtc aggtcggtag
    37621 caaggaaaat aaacgggaag ctaaacgcgc cccaggtggt atggaaacca aaaatggaga
    37681 cgggaagctg aaccagatag ttactggagg tgatcaccag cagatgaaat aacgataacc
    37741 agaacaacgc cttatagcgt tgagtttgcg agaaaacgtt catattgtac ctttttgatt
    37801 aaccattggg gtgagggaac ccaatacgta cgacacgtct gttaccttaa cataataacg
    37861 tgcttaccct tttttcgagc cgccgcatga tactgcttta cgttgacaat gcaatggtta
    37921 attttcacgc aatcgttaac ctggtttgct tacggactca cagggcgtaa actagcgccg
    37981 tttttttatg tgatgagaag aaaatgaccg atctcttttc cagccctgac cacacactcg
    38041 acgcacttgg cctgcgctgc ccggaacccg taatgatggt gcgcaaaaca gtgcgcaata
    38101 tgcagcctgg tgaaacgttg ctgattattg ctgacgatcc ggcgacgacc cgcgatattc
    38161 ctgggttttg tacctttatg gaacacgaac tggttgctaa agagacggat ggactgcctt
    38221 atcgttattt gattcgtaaa ggcggttgag gtggatcacg gactgttacc ctaaccctct
    38281 ccccagaggg gcgagtggac cgatcgcgct caatgttgcg atcggtttgc cttatctcct
    38341 gcgcaacaat cttaacgcat tcgctgtcac cagcaccgtc gcacccgtat ctgccagcac
    38401 cgccagccat agtccggtca ttcctaacag cgtggtgacg aggaagatcc ctttcagccc
    38461 cagcgcaata gtgatgttct ggcggatatt ggcgtgagtg gcgcgtgcca gttcaatcat
    38521 ttgcaccaga ccgcgcaggt ggttatgggt taatgctgcg tcggcggttt ccagcgccac
    38581 gtctgtgccg ctgcccattg caatcccgat ggcggcagct ttcattgctg gcgcgtcgtt
    38641 aataccgtca ccgaccatcg ccagcggcgc atgttgattc agctcggtca ccgctttgac
    38701 tttatcttcc ggcaacaggc ccgctttaaa ctccagcccc agctccccgg caattgccgc
    38761 cgctgcgcgt ggattatcgc cggtgaggat cacccctttg acgcccagcg cgttcagttc
    38821 actgatggca gttgcagcat cggcgcgcag ggtatcctgt aacgcaatga cacccagcac
    38881 gtcatcgtta cgtactacca gcactaccgt ttgcccggcg ctttccagtt cgttaattaa
    38941 accagcaaat gcatcagcgg gatgtttccc ggcagcgcaa atcaatacgc gctcaccgtt
    39001 aacctgcgct tcaatgccag acccgaccag cgcccgctgt gattcggcgg cgggaatggc
    39061 gagtgcagca acctgtgctt cgcgcacgat ggcctgcgcc agtggatgcg tcgcgccttg
    39121 ctcgaccgcc gccgccagtg tcagcagttc agattcacta atacccgttg ccggatgaat
    39181 cgcggtaacg cgcggtttac cgacggtcag cgtaccggtt ttatcaaacg ccacctgggt
    39241 aacacgacct agctgctcca gcgccgcacc gcctttaatt aacgccccac gacgtgccgc
    39301 tgctgccagc ccggaggtaa tcgccgcggg cgtggagata actaacgcac acgggcagcc
    39361 aatcagcagc agcgtcagcc ctttataaat ccactcttgc cagctggcgg caaacagcag
    39421 cggcggcacc agcgttacca gcagggcgac ggccataatc gctggcgtgt aaatacggct
    39481 gaaacggtcg ataaaccgct caatgggagc gcgacgctct tcggcttctt cgatcagttt
    39541 cagaatccgg tcaatggcgc tggctcccgg ttcggacaat acttccaacg tcaccagacg
    39601 gtctacgctg gtggcgcctg caggaacttt atcgcccgtc gcacgctcca ccggaatgga
    39661 ttcgccggtc agggcgcttt catcaaaact ggcaaagggt gagagcagtt taccgtcggc
    39721 aggcaaacgc ccacccgccg caacttcaat cacatcgcca gggcgcaggc tgttaattgc
    39781 cacctcttca cgctcaccgt tacgcaggcg cgtggcgttt tctggtttca gcgccattaa
    39841 cgcgctgacg ccctgacgcg cgcggctggc ggcccaacct tccagtcgtt caccaatcaa
    39901 aaacagcagc aacaccatcg cagcttcagc cgttgcgcca ataaacagcg caccaatagc
    39961 agctacgctc attaaggttt caatggcgaa gtagctgccg gatttgatca gccgtaatgc
    40021 ctgacgagca atcgggtaca gcccaaccag cgtggtcgcg ataaacgcca gttgcccgaa
    40081 cggatgattg aactgctcca gaccccagct gattgccatc atcacgatta gcgtaatcag
    40141 cggcagattc tctttcaggc gcgattcttg tggctcgtcg gcggcctgct catcgcgcag
    40201 ggaatagccc gctttttgca ccgcagattc aacctgggca cggatgtcat tgtcggcatc
    40261 aaccaccagt ttttcggtgg cgaacagtac ctgcacctga ttcacgcctg caagctggcg
    40321 cacggcattt tctaccttgc gcgcacaggc ggcgcagtcc atgccgctga ctttccagct
    40381 atagcgggtg ccggagacgt tttcagagag cgttggcgtg ctggaacatg cgccgtcgca
    40441 gcaacagtcg ttggcgttct gtaccgtggt tagcggtttg aacgcagcaa attgaggggc
    40501 tttcttgccg tgattgtcag gagtcgacat ggcatcctcc ggttaagttt tttctcatta
    40561 accgaaggat acactctgga gtcgactcca gagtcaagtt ttatcagaga tacagcgagc
    40621 ggacgatgag gaagtgaccg gcaaagtagc aggccgcagc aatcgcgtta tccgcacgga
    40681 agcgtcggcg atagtggcta cccagccaga caaagttact gataaacagc aacgaagcgc
    40741 cgacaaacgc ggagagcgcc ggagcggtcg gacggaagaa ccacagttca cctgccagcc
    40801 acaccatcac cagcgtcatg ccgataaacg tgcagatggg ccaacgatac tcttccagcc
    40861 gcgtccagat aatcgccagt aacagcgcgc ccagaaccag cagtaccaac ggcagaggcc
    40921 agaagaaaga gagcgtcatc tgactggcga aatagatggt gtacagcagg tgcgagagga
    40981 aaaacgcgcc gatggcgtac atcagacgtt gacgtggcaa cagggttagc gcatcgccca
    41041 gcagtgaggc gcacagcccc gccagcacca gatagctaat ggcgtcgaac atcggcgctt
    41101 gccaggccag taacaacagg agaagaaggg ttaacggttt aaacacccag cgttgccagg
    41161 ttggaccacg atacgatgca tccacagata gccatgcgga aagacagaca gcgataaacg
    41221 accaaagcat cttagttcct tgaattcgtt attttttcgt aagcggaatg cctacggtct
    41281 tgcttccagt ttagtggcga gggtggacgg atgacaacac cataactggc agggtatgct
    41341 taattccgca ttttccgatg agggataaag atgagcaaac cacctctttt ctttattgtt
    41401 atcattggct taatcatcgt cgccgcatcg tttcgtttta tgcaacagcg acgggaaaaa
    41461 gctgataatg atatggcacc gctccagcaa aagctggtgg tggtgagcaa caagcgggaa
    41521 aaaccgatta acgatcgccg ttcgcgccag caggaagtga ctccggcagg caccagtatg
    41581 cgctatgagg caagcttcaa accgcaaagc ggaggaatgg agcagacgtt tcgcctcgac
    41641 gcccagcagt accacgccct gacagtgggc gataaaggta cgctgagcta taaaggaacg
    41701 cgctttgtca gctttgtagg caaacaataa ggttattttt taactttaaa tttcttctgc
    41761 cagaccagca gttcaaacac gccgaaaagg aaaatccgca ccttttcggc ggtggtcatc
    41821 tgcgggccat ctttcggcaa ggtcgatttc aacagcgcca gctgcatacc atgcatcagc
    41881 acggtaaaaa tcagcgccac gttaacgaaa atattcagcg ggcgcgggaa gggatgcacc
    41941 aggttgagga ttaaaaatcc ccaaacgcag agcattaaca aacgtccaat attaatcagc
    42001 atcactttct ccttgtgctt cgcgttgata cagccgataa gccacctgac ccgccacttt
    42061 ttcccgatgc aatgaccagt ttgccggaac agtgggcaga ccgttttcga cttcgctttc
    42121 gacataaatc agggcttcgt cagccagcca gccgttatct tccagtaaat ttatcgtctc
    42181 ttctaacaag ccacggcgga acggtggatc gacaaacacg atattatgcg gtgtgccttt
    42241 ttgcgccagg aatgacatcg cgttgctgtt caccacgcgt gcattgcctg cttttagtgt
    42301 cgccagattc ttaattaact gctgagaaac tgcacgatcc atctcaatca acgtggcccc
    42361 cgcagcgtag cgtgataacg cttccagccc cagcgcgccg ctgccggcga agcaatccag
    42421 acattgggcg tcaacaatga ccggagccag ccagttaaac aacgtttcgc gtacgcggtc
    42481 ggtggtgggg cgcagacctg ggctatcagg aaccgggagt ttacggcctc gccactgccc
    42541 gccaataatg cggatttggc cgctgccgga atgattcggt tttttcatga taaattgctc
    42601 aatccgccag ataacacata cttgcaggcg gtgatgtgaa ttaagttaag tgatagacta
    42661 tttcatcatt tttttagctg ctatgtacat agcgttaacg ctgtgccatg aagcaacagc
    42721 gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg ctttttttcc tggctgggct
    42781 ttggtcaaaa agagcagacc ccggaaaaag agacagaagt tcagaatgaa caaccggttg
    42841 tagaagaaat cgttcaggcg caagagcctg cgaaggcttc ggaacaagcc gttgaagagc
    42901 agccgcaggc gcatactgaa gccgaggcgg aaacttttgc tgccgacgtt gtggaagtca
    42961 ctgaacaggt tgctgaaagt gaaaaagcgc agccggaagc ggaagtcgtt gcacagccgg
    43021 aaccggtcgc agaagaaacg ccggagccag tggctatcga acgtgaagaa ctgccgttgc
    43081 cggaagacgt caacgccgaa gaggtttcgc cagaagagtg gcaggccgaa gcggaaaccg
    43141 tagagattgt cgaagcggcg gaagaagaag cggctaaaga agaaatcacc gacgaagagc
    43201 tggaaacaca ggcgctggct gccgaagcgg cagaagaggc ggtgatggtg gttcctctgg
    43261 cagaagaaga gcagccggtg gaagaaatcg ctcaggagca ggaaaagccg accaaagaag
    43321 gtttcttcgc gcgcctgaaa cgcagcctgt taaaaaccaa agaaaacctc ggttccggat
    43381 ttatcagcct gttccgcggt aaaaaaatcg acgatgatct ttttgaagag ctggaagaac
    43441 agctgttgat cgccgatgtg ggcgtggaaa ccacacgtaa aattatcacc aatctgacgg
    43501 aaggcgcatc ccgcaagcag cttcgtgatg ccgaggcgct ctatggcctg ctgaaagaag
    43561 agatgggcga gattctggcg aaagtcgatg agccgctgaa tgttgaaggt aaaacgccgt
    43621 tcgtgatcct gatggtgggc gtcaacggtg tgggtaaaac cacgacgatt ggtaagctgg
    43681 cgcgtcagtt tgagcagcag ggtaaatcgg tgatgctggc ggcgggcgat actttccgtg
    43741 cagcagcggt tgaacagctt caggtctggg gtcagcgcaa caatattccg gtgattgccc
    43801 agcataccgg tgcggattcc gcctctgtta tcttcgacgc cattcaggca gctaaagcgc
    43861 gtaatatcga cgtcctgatt gccgataccg ccggacgtct gcagaacaaa tcgcatctga
    43921 tggaagagtt gaagaaaatc gtccgcgtga tgaagaaact cgacgttgaa gcgccgcatg
    43981 aagttatgct gactattgat gccagcaccg ggcagaacgc ggtaagccag gccaaactgt
    44041 tccacgaagc tgttggctta accggcatca cgctaacgaa actggacggc acggcgaaag
    44101 gcggggtaat tttctcggtg gctgaccagt ttggtatccc tatccgctac attggtgtcg
    44161 gcgaacgtat tgaggatttg cgtccgttta aggcggacga ctttatagag gcactttttg
    44221 cccgagagga ttaacaatga ttcgctttga acatgtcagc aaggcttatc tcggtgggag
    44281 acaggcgctg cagggcgtta cgttccatat gcagccgggt gagatggcgt ttctgaccgg
    44341 tcattccggc gcagggaaaa gtaccctcct gaagctgatc tgtgggattg agcggcccag
    44401 cgccgggaaa atctggttta gcggccatga catcacgcgt ctgaaaaacc gtgaagttcc
    44461 gtttctgcgc cgccagattg gcatgatttt ccaggatcac catctactga tggaccgtac
    44521 tgtctacgat aacgtggcga tcccgctgat tatcgccggt gccagcggtg acgatattcg
    44581 tcgccgggtg tcggcggcgc tggataaagt cgggctactg gacaaagcga agaactttcc
    44641 tattcagctt tccggcggtg aacaacagcg tgttggcatt gcccgcgcgg tggtgaacaa
    44701 gcctgcggta ctgctggcgg acgaaccgac tggtaacctg gacgacgcgt tgtcggaagg
    44761 gattctacgt ctgtttgaag agtttaaccg cgttggggta accgtattga tggcaacgca
    44821 cgacatcaac ctgatttcgc ggcgttccta tcgcatgctc accctgagcg atggtcactt
    44881 gcatggaggc gtgggccatg aataagcgcg atgcaatcaa tcatattcgg cagtttggcg
    44941 ggcgtcttga tcgcttccgt aaatcggtcg gcggctcagg cgacggcggt cgtaacgcac
    45001 cgaaacgcgc gaaatcctcg ccaaaaccgg taaatcgcaa aaccaacgtt ttcaacgaac
    45061 aggtgcgcta tgccttccac ggcgcattgc aggatctgaa aagcaaaccg ttcgccacgt
    45121 ttttaacggt gatggttatc gccatctccc tgacgctgcc cagcgtctgt tatatggtgt
    45181 acaaaaacgt taaccaggcg gcgacgcaat attatccgtc accgcaaatc actgtttatc
    45241 tgcaaaaaac gctggacgat gacgctgctg cgggcgtggt ggcacagttg caggccgagc
    45301 agggcgtgga gaaagtgaat tatctttctc gtgaagacgc actgggcgag ttccgtaact
    45361 ggtctggttt tggtggtgcg ctggatatgc tggaagaaaa cccgcttccg gcagtggcgg
    45421 tggtgatccc gaaactcgac tttcagggga cggagtctct gaatacgctg cgtgatcgta
    45481 tcacgcagat taacggcatt gacgaagtgc ggatggatga cagctggttt gcccgtctgg
    45541 cggcgttgac cgggctggtc gggcgcgttt cggcgatgat cggcgtgttg atggtggcgg
    45601 cagtgttcct cgtcatcggt aacagtgtac gcctgagtat ctttgctcgc cgtgactcca
    45661 tcaacgtgca gaaactgatt ggtgcgacgg atggtttcat cctgcgcccg ttcctgtacg
    45721 gtggcgcact gctgggattt tctggcgcat tgttgtcatt aattttgtca gaaattctgg
    45781 tgctgcgatt gtcatcggcg gttgcggaag tggcacaggt tttcggaacg aagtttgata
    45841 tcaatggctt atcgttcgat gaatgcctgc tattgctgct ggtatgctcg atgattggct
    45901 gggtggcagc gtggcttgcc acggtacaac atttacgcca ctttacgcct gaataataaa
    45961 agcgtgttat actctttccc tgcaatgggt tccgtagcag ggagagagac cccgttgtct
    46021 cttcccggta tttcatctct atgtcacatt ttgtgcgtaa tttattcaca agcttgcatt
    46081 gaacttgtgg ataaaatcac ggtctgataa aacagtgaat gataacctcg ttgctcttaa
    46141 gctctggcac agttgttgct accactgaag cgccagaaga tatcgattga gaggatttga
    46201 atgactgaca aaatgcaaag tttagcttta gccccagttg gcaacctgga ttcctacatc
    46261 cgggcagcta acgcgtggcc gatgttgtcg gctgacgagg agcgggcgct ggctgaaaag
    46321 ctgcattacc atggcgatct ggaagcagct aaaacgctga tcctgtctca cctgcggttt
    46381 gttgttcata ttgctcgtaa ttatgcgggc tatggcctgc cacaggcgga tttgattcag
    46441 gaaggtaaca tcggcctgat gaaagcagtg cgccgtttca acccggaagt gggtgtgcgc
    46501 ctggtctcct tcgccgtcca ctggatcaaa gcagagatcc acgaatacgt tctgcgtaac
    46561 tggcgtatcg tcaaagttgc gaccaccaaa gcgcagcgca aactgttctt caacctgcgt
    46621 aaaaccaagc agcgtttggg ctggtttaac caggatgaag tcgaaatggt ggcccgtgaa
    46681 ctgggcgtaa ccagcaaaga cgtacgtgag atggaatcac gtatggcggc acaggacatg
    46741 acctttgacc tgtcttccga cgacgattcc gacagccaac cgatggcacc ggtgctctat
    46801 ctgcaggata aatcatctaa ctttgccgac ggcattgaag atgataactg ggaagagcag
    46861 gcagcaaacc gtctgaccgc cgcgatgcag ggtctggacg aacgcagcca ggacatcatc
    46921 cgtgcgcgct ggctggacga agacaacaag tccacgttgc aggaactggc tgaccgttac
    46981 ggtgtttccg ctgaacgtgt acgccagctg gaaaagaacg cgatgaaaaa actgcgcgct
    47041 gctatagaag cgtaatttcc gctattaagc agagaaccct ggatgcgagt ccggggtttt
    47101 tgttttttgg gcctctacaa taatcaattt cccctccggc aaaacgccaa tccccacgca
    47161 gattgttaat aaactgtcaa aatagctatt ccaatatcat aaaaatcggg tatgttttag
    47221 cagagtatgc tgctaaagca cgggtagtca tgcgtaaaac gaaataaagt gctgaacaac
    47281 aacatcacaa cacacgtaat aaccagaaga atggggattc tcaggatgaa cataaagggt
    47341 aaagcgttac tggcaggatg tatcgcgctg gcattcagca atatggctct ggcagaagat
    47401 attaaagtcg cggtcgtggg cgcaatgtcc ggtccggttg cgcagtacgg tgaccaggag
    47461 tttaccggcg cagagcaggc ggttgcggat atcaacgcta aaggcggcat taaaggcaac
    47521 aaactgcaaa tcgtaaaata tgacgatgcc tgtgacccga aacaggcggt tgcggtggcg
    47581 aacaaagtcg ttaacgacgg cattaaatac gtgattggtc acctctgttc ttcatcaacg
    47641 cagcctgcgt ctgacatcta cgaagacgaa ggcattttaa tgatcacccc agcggcaacc
    47701 gcgccggagc tgaccgcccg tggctatcag ctgatcctgc gcaccaccgg cctggactcc
    47761 gaccaggggc cgacggcggc gaaatatatt cttgagaaag tgaaaccgca gcgtattgct
    47821 atcgttcacg acaaacagca atacggtgaa ggtctggcac gagcggtgca ggacggcctg
    47881 aagaaaggca atgctaacgt ggtgttcttt gatggtatca ccgccgggga aaaagatttc
    47941 tcaacgctgg tggcgcgttt gaagaaagag aatatcgact tcgtttacta cggcggttat
    48001 cacccggaaa tggggcaaat cctgcgtcag gcacgcgcgg cagggttgaa aactcagttt
    48061 atggggccgg aaggcgtggc taacgtctcg ctgtctaaca ttgcgggcga atcagcggaa
    48121 gggctgctgg tgaccaagcc gaagaactac gatcaggttc cggcgaacaa acccattgtt
    48181 gatgcgatca aagcgaaaaa acaggaccca agcggcgcat tcgtttggac cacctacgcc
    48241 gcgctgcaat ctttgcaggc gggcctgaat cagtctgacg atccggctga gatcgccaaa
    48301 tacctgaaag cgaactcggt tgataccgtg atgggtccgc tgacgtggga tgagaaaggt
    48361 gatctgaaag gctttgagtt cggtgtgttt gactggcacg ccaacggcac agcgaccgat
    48421 gcgaagtaat tgttaatcgg caactttggg ttgccgccaa attgctaata tagagtacgt
    48481 tgcttcatgc cagatgcggc gtaaacgcct tatccggcct acaagatcca aagaaatcag
    48541 taaattgcaa cacacattgt aggcctgata agcgtagcgc atcaggcaat ttctcgttgg
    48601 tcatcacttt tgaaatcaaa cttgacgatt aacgcttctc ccagccgccc tgttgtgccg
    48661 taaaccccag tgcctgcata aacgctgtca tcacaccgcg atcttccacg cctgcatccg
    48721 ccatccacca gcatgaaacg ccaggattgt tacgcaaaac ctcttccagc agatattgcc
    48781 ccacaccgcg acgtcgagtg acttcccgca cgcgcaggga atccagtgct ccctcggtgc
    48841 cgcttaaggt tacccgtacg gcagcgagca ggcgctcgtt aaaacgcgcg gcgtagatac
    48901 ggtggttatc gtcaacctgt aacgaggaag gggaatactc cggccagatc ttttgcaggt
    48961 caatccggtc ctggtcgcta aatttttcta atcgaatgat ggtcagcttc atgggtaacc
    49021 cgtgtaaatc acaaaagtgg aaccagtgta gcgaaataat ttaatcggag gctttctctt
    49081 ttttatttct tttggcaggt gattaatttt ttaacagcaa taattacaaa gttaaaacat
    49141 tagagaatga aaaataccca gcataatccc ctgaatggta gtgaattatt ccgccctttg
    49201 tgccgttatt ttatgctgac aacggcactt ttttctgttt atctatcaat aaattcagaa
    49261 tattatctgt tcttaatcga ctgaaaaata gggactttaa tcgctattat cacaaaatac
    49321 tgcgctaaac ccttaatcag acaggcaaaa acagtgcagt ataaaaaaag aacagtctga
    49381 tttgttaaca cataaaaaca aagcaacaca acatcacgaa tggggatttt tgactatgaa
    49441 acggaatgcg aaaactatca tcgcagggat gattgcactg gcaatttcac acaccgcttt
    49501 agccgacgat attaaagttg ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg
    49561 cgatatggaa tttaacggcg cgcgtcaggc gattaaagac attaatgcca aagggggaat
    49621 taaaggcgat aagctggttg gcgtggaata tgacgacgcc tgcgacccga aacaagccgt
    49681 tgcggtcgcc aacaaaatcg ttaatgacgg cattaaatac gttattggtc atctgtgttc
    49741 ttcttctacc cagcctgcgt cagatatcta tgaagacgaa ggtattctga tgatctcgcc
    49801 gggagcgacc aacccggagc tgacccaacg cggttatcaa cacattatgc gtactgccgg
    49861 gctggactct tcccaggggc caacggcggc aaaatacatt cttgagacgg tgaagcccca
    49921 gcgcatcgcc atcattcacg acaaacaaca gtatggcgaa gggctggcgc gttcggtgca
    49981 ggacgggctg aaagcggcta acgccaacgt cgtcttcttc gacggtatta ccgccgggga
    50041 gaaagatttc tccgcgctga tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta
    50101 cggcggttac tacccggaaa tggggcagat gctgcgccag gcccgttccg ttggcctgaa
    50161 aacccagttt atggggccgg aaggtgtggg taatgcgtcg ttgtcgaaca ttgccggtga
    50221 tgccgccgaa ggcatgttgg tcactatgcc aaaacgctat gaccaggatc cggcaaacca
    50281 gggcatcgtt gatgcgctga aagcagacaa gaaagatccg tccgggcctt atgtctggat
    50341 cacctacgcg gcggtgcaat ctctggcaac tgcccttgag cgtaccggca gcgatgagcc
    50401 gctggcgctg gtgaaagatt taaaagctaa cggtgcaaac accgtgattg ggccgctgaa
    50461 ctgggatgaa aaaggcgatc ttaagggatt tgattttggt gtcttccagt ggcacgccga
    50521 cggttcatcc acggcagcca agtgaaaatc ccaccgcccg tgaaatgcgg gcgggtttag
    50581 aaaggttacc ttatgtctga gcagttttta tatttcttgc agcagatgtt taacggcgtc
    50641 acgctgggca gtacttacgc gctgattgcc atcggctaca ccatggttta tggcattatc
    50701 ggcatgatca acttcgccca cggcgaggtt tacatgatcg gcagctatgt ctcctttatg
    50761 atcatcgccg cactgatgat gatgggcatt gataccggct ggctgctggt agcggcggga
    50821 ttcgtcggcg caatcgtcat tgccagcgcc tacggctgga gtatcgaacg ggtggcttac
    50881 cgcccggtgc gtaactctaa gcgcctgatt gcactcatct cggcaatcgg tatgtccatc
    50941 ttcctgcaaa actacgtcag cctgaccgaa ggttcgcgcg acgtggcgct gcccagcctg
    51001 tttaacggtc agtgggtggt gggccatagc gaaaacttct ctgcctctat tactaccatg
    51061 caggcggtta tctggattgt tactttcctc gccatgctgg cgctgacgat tttcattcgc
    51121 tattcccgca tggggcgcgc gtgtcgtgcc tgtgcggaag atctgaaaat ggcgagcctg
    51181 cttggcatta acactgaccg ggtgattgcg ctgacctttg tgattggcgc ggcgatggca
    51241 gcggtggcgg gcgtactgct cggtcagttc tacggcgtca ttaaccccta tatcggcttt
    51301 atggccggga tgaaagcctt taccgctgcg gtgctcggtg ggattggcag cattccggga
    51361 gcgatgattg gcgggctgat tttggggatt gcggaggcgc tctcttctgc ctatctgagt
    51421 acggaatata aagatgtggt ctcattcgcc ctgctgattc tggtgctgct ggtgatgccg
    51481 accggtattc tgggtcgccc ggaggtagag aaagtatgaa accgatgcat attgcaatgg
    51541 cgttgctctc tgccgcgatg ttctttgtgc tggcgggcgt ctttatgggc gtgcaactgg
    51601 agctggatgg caccaaactg gtggtcgata gcgcttcgga tgtccgttgg cagtgggtgt
    51661 ttatcggcac ggcggtggtc tttttcttcc agcttttgcg accggctttc cagaaagggt
    51721 tgaaaagcgt ttccggaccg aagtttattc tgcccgcgat tgatggctcc acggtgaagc
    51781 aaaagctgtt tctcgtggcg ctgctggtgc ttgcggtggc gtggccgttt atggtctcac
    51841 gcgggacggt ggatatcgcc accctgacca tgatctacat tattctcggc ctcgggctga
    51901 acgtggttgt tggtctttct ggtctgctgg tgctggggta cggcggtttt tacgccatcg
    51961 gcgcttacac ttttgcgctg ctcaatcact attacggtct ggggttctgg acctgtttgc
    52021 cgattgccgg gttgatggca gcggcggcgg gcttcctgct cggcttcccg gtgcttcgct
    52081 tgcgcggcga ctatctggcg atcgttaccc tcggtttcgg cgaaattgtg cgcatattgc
    52141 tgctcaataa caccgaaatt accggcggcc cgaacggaat cagtcagatc ccgaaaccga
    52201 cactcttcgg actcgagttc agccgtaccg ctcgtgaagg cggctgggac acgttcagta
    52261 atttctttgg cctgaaatac gatccctccg atcgtgtcat cttcctctac ctggtggcgt
    52321 tgctgctggt ggtgctaagc ctgtttgtca ttaaccgcct gctgcggatg ccgctggggc
    52381 gtgcgtggga agcgttgcgt gaagatgaaa tcgcctgccg ttcgctgggc ttaagcccgc
    52441 gtcgtatcaa gctgaccgcc tttaccatca gtgccgtgtt tgccggtttt gccggaacgc
    52501 tgtttgctgc gcgccagggc tttgtcagcc cggaatcctt cacctttgcc gaatcagcct
    52561 ttgtactggc gatagtggtg ctcggcggta tgggctcgca atttgcagtg attctggcgg
    52621 ctgttctgct ggtggtatcg cgcgagttga tgcgtgattt caacgaatac agcatgttaa
    52681 tgctcggtgg tttgatggtg ctgatgatga tctggcgtcc gcagggcttg ctgcccatga
    52741 cgcgcccgca actgaagctg aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc
    52801 attattatct gttaacggcc tgatgatgcg cttcggcggc ctgctggcgg tgaacaacgt
    52861 caatcttgaa ctgtacccgc aggagatcgt ctcgttaatc ggccctaacg gtgccggaaa
    52921 aaccacggtc ttcaactgcc tgaccggttt ctacaaaccc accggcggca ccattttact
    52981 gcgcgatcag catctggaag gtttgccggg gcagcaaatc gcccgcatgg gcgtggtgcg
    53041 taccttccag catgtgcgtc tgttccgtga aatgacggta attgaaaacc tgctggtggc
    53101 gcagcatcag caactgaaaa ccgggctgtt ctcaggcctg ttgaaaacgc cgtctttccg
    53161 tcgcgcccaa agtgaagcgc tcgaccgcgc cgccacctgg cttgagcgca ttggtttgct
    53221 ggaacacgcc aaccgtcagg cgagtaacct ggcctacggt gaccagcgcc gtctggagat
    53281 cgcccgctgt atggtgacgc agccggagat tttaatgctc gacgaaccgg cagcaggtct
    53341 taacccgaaa gagacgaaag agctggatga gctgattgcc gaactgcgta accatcacaa
    53401 caccactatc ttgttgattg aacacgatat gaagctggtg atggggattt cagacagaat
    53461 ctacgtggtc aaccagggga cgccgctggc aaacggtacg ccggagcaaa tccgtaataa
    53521 cccggacgtg atccgtgcct atttaggtga ggcataagat ggaaaaagtc atgttgtcct
    53581 ttgacaaagt cagcgcccac tacggcaaaa tccaggcgct gcatgaggtg agcctgcata
    53641 tcaatcaggg cgagattgtc acgctgattg gcgcgaacgg ggcggggaaa accaccttgc
    53701 tcggcacgtt atgcggcgat ccgcgcgcca ccagcgggcg aattgtgttt gatgataaag
    53761 acattaccga ctggcagacg gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag
    53821 ggcgtcgcgt cttctcgcgg atgacggtgg aagagaacct ggcgatgggc ggcttttttg
    53881 ccgaacgtga tcagtttcag gagcgcataa agtgggtgta tgagctgttt ccacgtcttc
    53941 atgagcgtcg tgttcagcgg gcgggcacca tgtccggcgg tgaacagcag atgctggcga
    54001 ttggtcgcgc gctgatgagc aacccgcgtt tgttgttgct tgatgagcca tcgctcggtc
    54061 ttgcgccgat tatcatccag caaattttcg acaccatcga gcagctgcgc gagcagggga
    54121 tgactatctt cctcgtcgag cagaacgcca accaggcgct gaagctggcg gatcgcggct
    54181 acgtgctgga aaacggccat gttgtgctct ccgataccgg cgatgcgctg ctggcgaacg
    54241 aagcagtgag aagtgcgtat ttaggcgggt aaattatcga atacccggcg gccacgttct
    54301 gccgggctct tcgtccgtat acagacttgt ttcaactgac cgggaggatt aaaaccagac
    54361 cacttatttg ccacggaaag gttccctccc atgcattatg ccgttgttgt catgcttgaa
    54421 ctgctttgct gtattcacgc ttaccgtacc ggacaagagc ggtactggat ttttatcatt
    54481 ttctgttttc cggtgattgg ctgcgtggcg tatttcgtca tagtgatgct gcctgaaacg
    54541 ggcgctgacc gtcacgggca tactttgctg atgcgcctgc agaacaaact caaccctgag
    54601 cgccacctgc gcaaattaac cgaagaactg gcgattgccg agacgaatca aaaccattat
    54661 gcgttggcta atgaactggc gcgtctgggg cgttatcacg aagctgttcc ccattatcag
    54721 caggcgttaa gcgggatatt cgcccatgaa gcggcaatga tgctaagcct ggcgcaggca
    54781 cagtttgcta ttcaggagtt cgccgcctgt cagcagacgc tggaagatgt gatgcgttat
    54841 aaccctgatt ttcagtcggc agacgggcat ttgttatttg ccagaacgtt agccgcacaa
    54901 gaaaaatacg ctgatgcgga aagcgagttt gaagtattga tttcttatta ccccggcccg
    54961 caggcgcgta tttactatgc ggaaatgttg gcgaaaatga gtcggttacg tgaagccaac
    55021 gagcagtatg ttgctgtagt tgatacggct aaacgctcaa gacctcatta tagaaaacac
    55081 caccgggaat ggattaagac cgccaatgaa cggctgaagc agagtgtggt gcaatgaaaa
    55141 tcttctttgc cgttagacca gtgtcgcggc aatcttttgt taaaattgaa aaatgtagct
    55201 ctcatcgggc agcaaaactg gacgcagaat ttgctgccat ttttgctctt catggtaagg
    55261 agttctggcg attagctggc cgttaatcgc ctcttttcag tgcattctcg ggatatccgt
    55321 gccccatctc ccattttccc tcacccgtac cgtcaccgcc ttgtcatctt tctgacacct
    55381 tactatctta caaatgtaac aaaaaagtta tttttctgta attcgagcat gtcatgttac
    55441 cccgcgagca taaaacgcgt gaattcgcgc attcggtaca acaagagaga taaacgatga
    55501 aaccgttaca ttatacagct tcagcactgg cgctcggact ggcgttaatg gggaatgcac
    55561 aggcagtgac gaccattccg ttctggcatt ctatggaagg ggaactgggt aaagaggtgg
    55621 attctctggc ccaacgtttt aacgccgaaa acccggatta caaaattgta ccgacctata
    55681 aaggcaacta cgaacagaat ttaagcgcgg ggattgccgc atttcgtacc ggcaatgctc
    55741 cggctatttt gcaggtttat gaagttggca ccgccaccat gatggcgtcg aaagccatta
    55801 aaccggtata tgacgtgttt aaagaggcgg ggattcaatt cgatgagtcg cagtttgtgc
    55861 cgacggtttc cggttactac tccgacagca aaacgggcca cttactctcc cagccattca
    55921 acagctcgac ccccgttctc tattacaaca aagacgcctt caagaaagca ggattagacc
    55981 cggaacagcc gccgaaaacc tggcaggatc tggcggacta ttctgcaaaa ctgaaagcct
    56041 ccggaatgaa gtgcggctac gccagcggct ggcagggctg gatccaactg gaaaacttta
    56101 gcgcctggaa cggtctgccg tttgccagca aaaacaacgg ctttgacggc acggatgcgg
    56161 tgctggagtt caacaagccg gagcaggtga aacacatcgc catgctcgaa gagatgaaca
    56221 agaagggcga tttcagctac gtcgggcgta aggatgaatc caccgagaag ttctataacg
    56281 gtgattgcgc gatgaccacc gcctcttccg gttctctcgc taatattcgt gagtacgcca
    56341 aatttaacta tggcgtaggc atgatgcctt acgacgccga tgcgaaagat gcaccgcaaa
    56401 acgccattat cggcggggcc agcctgtggg tgatgcaggg caaagataaa gaaacgtata
    56461 ccggcgtggc gaagttcctc gacttcctcg cgaagccaga aaacgctgcc gagtggcatc
    56521 agaaaaccgg ttatctgcca atcaccaaag cagcgtatga cctgacccgt gagcagggct
    56581 tttatgagaa aaacccaggg gcggataccg cgacgcgtca gatgctgaac aagccgccgt
    56641 tgccgttcac caaagggctg cgtctgggca acatgccgca gatccgcgtg attgtggatg
    56701 aagagctgga gagcgtgtgg accggtaaga agacaccgca gcaggcactg gataccgccg
    56761 ttgagcgtgg gaaccagtta ctgcgccgct ttgagaaatc gacgaagtct taatcagtgt
    56821 aatgtcggat gcgtttcgct tatctgacct ggcatcgcgt gtaggccgga taagcgaagc
    56881 gcatccggca cagttcagga attaaccgta atgtcatcat cccgtccggt gttccgcgcg
    56941 cgctggctgc cttatctgct ggtcgcgccg cagctcatca tcaccgttat cttttttatc
    57001 tggcctgcgg gcgaagcgtt gtggtactcg ctgcaaagcg tcgatccgtt tggtttctcc
    57061 agccagtttg tcggcctgga taacttcgtc gcgctgtttc atgacagcta ctatctcgac
    57121 tccttctgga cgacgataaa attcagcacc tttgtcaccg tcagcggttt gctggtatcg
    57181 ctgttctttg cggcgctggt ggagtacatc gtgcgcggca gtcgtttcta tcaaaccttg
    57241 atgctgctgc cgtatgccgt ggctcccgcc gttgccgccg tattgtggat cttcctgttt
    57301 aaccccggtc gcgggctgat cacccatttt ctcgctgagt tcggctacga ctggaaccac
    57361 gcgcaaaaca gcggtcaggc aatgtttctg gtggtgtttg cctcagtatg gaagcaaatc
    57421 agctacaact tcctgttctt ctatgccgcg ctgcaatcca ttccccgttc gttgatcgaa
    57481 gccgcagcca tcgacggtgc agggccgatt cgccgcttct ttaagattgc gctgccgctt
    57541 atcgctccgg tgagtttctt cctgctggtg gtgaacctgg tgtacgcctt cttcgacacc
    57601 ttcccggtga tcgacgccgc cacgtccggc gggccggttc aggccaccac gacgctgatt
    57661 tataagatct accgcgaagg ctttaccgga ctggatctgg cttcgtctgc cgcgcagtcg
    57721 gtggtgttga tgttcctcgt catcgtgctg acggtggtgc agttccgcta tgttgaaagc
    57781 aaggtacgtt accaatgatt gagaaccgtc cgtggctgac gatattcagc cataccatgc
    57841 tgatcctcgg gatcgcggtg atcctcttcc cgctgtacgt ggcgtttgtc gcggcgacgc
    57901 tggataaaca ggccgtctac gccgcgccga tgacgctcat ccccggcacg catctgctgg
    57961 aaaacatcca caacatctgg gtaaacgggg taggcacaaa tagtgcgccg ttctggcgga
    58021 tgttgcttaa cagcttcgtg atggcgttca gcattacgct cggcaaaatt accgtctcga
    58081 tgctctcggc attcgccatt gtctggtttc gttttccgct gcgtaacctc tttttctgga
    58141 tgatttttat caccctgatg ttgccggttg aagtacgtat cttcccgacg gtggaagtca
    58201 tcgccaacct gaagatgctc gacagctacg caggtttaac gctgccgctg atggcctcgg
    58261 cgaccgcgac gtttcttttc cgccagttct ttatgaccct accggatgaa ctggtggaag
    58321 ccgcgcggat cgacggcgcg tcgccaatgc gcttcttttg cgacatcgtt tttccgttgt
    58381 ccaaaactaa tctggcggcg ttgtttgtga tcacctttat ttacggctgg aaccagtatt
    58441 tgtggccgtt gttgattatt accgacgtgg atcttggcac caccgtggca gggatcaaag
    58501 ggatgatcgc cactggcgaa ggcaccacgg aatggaactc ggtgatggct gctatgttgt
    58561 taacgcttat ccctccggtg gtgattgttt tagtgatgca gcgtgccttc gtgcgcggcc
    58621 tggtcgatag tgagaaataa gatggcagga ctgaaattac aggcagtaac caaaagctgg
    58681 gatggcaaaa cccaggtcat taaaccgctg actcttgatg tggcggatgg cgaatttatc
    58741 gtgatggtcg ggccgtctgg ctgcgggaaa tcgacgctgc tgcgcatggt tgccgggctg
    58801 gagcgggtga cggaaggcga tatctggatc aacgaccagc gggtgaccga aatggagccg
    58861 aaagatcgcg ggattgcgat ggtgttccag aactacgcgc tttatccgca tatgagtgtt
    58921 gaagaaaaca tggcgtgggg gctgaaaatt cgcggcatgg gcaagcagca aattgccgag
    58981 cgcgttaaag aagcggcgcg cattctggaa ctggacggtc tgcttaagcg ccgcccgcgc
    59041 gagctttccg gcggtcagcg tcagcgtgtg gcgatgggcc gcgcgattgt gcgcgatccg
    59101 gcggtgttcc tgtttgatga gccactctct aacctcgatg ccaagctgcg cgtacagatg
    59161 cgtcttgaac tgcaacagct gcaccgtcgc ctgaaaacga cttcactcta cgttactcac
    59221 gatcaggttg aggcgatgac cctcgcccag cgagtaatgg tgatgaacgg cggcgttgcc
    59281 gaacagattg gcacgccagt tgaagtctac gaaaagcccg ccagcctgtt tgtggcgagt
    59341 tttattggca gtccggcgat gaatctgctg acaggccgcg tgaataatga aggcacgcat
    59401 tttgaactgg acggcggtat cgtgctaccg ctaaacggtg gctaccgtca gtatgccggg
    59461 cgtaaaatga ctctcggcat tcgcccggaa catattgcgc taagctcgca ggcagaaggc
    59521 ggcgtaccgc tggtgatgga cacgctggag atcctcggcg cagataacct ggcgcacgga
    59581 cgctggggcg aacagaagct ggtggtgcga ctggcgcatc aggagcgccc gacggcaggc
    59641 agcacgctgt ggctgcatct ggcggaaaat cagctgcatc tttttgatgg tgaaacagga
    59701 caacgagtat gagtaactgg ccttatcccc gcatcgtcgc acatcgtggc ggcggtaagc
    59761 tggccccgga aaacaccctg gcggcaatcg acgtcggggc aaaatacggt cataagatga
    59821 tcgaatttga cgcgaagtta tcgaaagatg gcgagatctt tctgctccat gacgacaatc
    59881 ttgaacgtac cagcaacggc tggggcgtcg cgggtgaact gaactggcag gatttactac
    59941 gtgtggatgc aggcagttgg tacggcaaag cgtttaaagg tgagccgctg ccgttgcttt
    60001 cgcaggtggc ggaacgctgt cgcgaacatg ggatgatggc gaatatcgaa atcaaaccca
    60061 ccactggcac cgggccatta acgggcaaaa tggtggcgct ggcggcacgc gagctgtggg
    60121 ccggtatgac gccgccgcta ctgtcatcgt ttgagattga tgctttagaa gccgcacaac
    60181 aggcagtacc agaactgccg cgcggtttgt tgctggatga gtggcgcgac gactggcgag
    60241 aactgaccgc gcggctgggc tgcgtctcta ttcatctcaa tcataagtta ctcgataaag
    60301 cgcgagtgat gcagttgaaa gacgccggat tacggattct ggtttatacc gtcaataaac
    60361 cccagcgcgc ggcagagttg ctgcgctggg gcgtggattg catctgtacc gatgcgattg
    60421 atgtgattgg tccgaacttt acggcccaat agttttcaac ggtatatcgg gctgcggcgt
    60481 gccgggctga ttcagcatgt cgccgtttct cctctccggc aacatatgct gctgattcaa
    60541 cgaactatcc ggattacggt tgctgtttaa catcccgcca ttggtgttgg gcagcatttg
    60601 ctgccgggcg ggatttcgct cccccggctg tgactgcaac acccgctgag aattattgtt
    60661 tatctggttt tctaaatgct gttgttgcaa ctgagtttgc gttttcagtt gctgattcag
    60721 catccctttt tgctggattt gctgagtctg catctgggtt tgcatccgct gctgactggg
    60781 gatctgatac cccggctggt tagggttgtt cagagtatta atgggctgtg caaagccgac
    60841 aaacggcagg agtgccgtaa gaatcagaag tcgtttcatc gcgtatcctc ctctgaagat
    60901 attctttaag tttactcctt tcctgcaatc atcaggatta attcagagtt atataccagg
    60961 cttagctggg gttgcccctt aatctctgga gaataacgat gataaaaccg acgtttttac
    61021 gccgggtggc tattgctgct ctgctctcag gaagttgttt tagcgccgcc gccgcgcctc
    61081 ctgtttctta cggcgtggag gaagatgtct tccatccggt acgcgcgaaa caggggatgg
    61141 tggcatctgt agatgctact gccactcagg tgggggtgga tatcctcaag gagggcggga
    61201 atgccgttga tgccgccgtg gctgtgggct acgcgctggc ggtaacgcat ccgcaggcgg
    61261 ggaatctggg cggcggtggt tttatgttaa tccgctcgaa aaatggcaat accaccgcta
    61321 tcgatttccg cgaaatggca cccgccaaag cgacccgcga tatgttcctt gatgatcagg
    61381 gtaacccgga cagcaaaaaa tcactcactt cgcatctggc ttccggcacg cccggtacgg
    61441 tcgctggttt ctcgctggcg ttggataaat acggcacaat gccgttgaac aaagtggtgc
    61501 agccagcgtt taaactggca cgcgatggtt ttatcgttaa cgacgcgctg gctgacgatc
    61561 tcaaaaccta cgggagcgaa gtgctgccga atcacgaaaa cagcaaggct atcttctgga
    61621 aagagggcga gccgctgaaa aagggcgaca agctggtgca ggcgaacctg gcaaagagcc
    61681 tggagatgat tgccgaaaac ggcccggacg aattctacaa aggcacgata gcggaacaga
    61741 ttgcccatga gatgcagaaa aacggtggcc tgatcactaa agaagattta gcggcgtata
    61801 aagcggtcga acgcacaccg ataagcggcg attatcgcgg gtatcaggtt tactccatgc
    61861 caccgccatc ctccggtggg attcatatcg tgcagatcct caatattctg gaaaacttcg
    61921 atatgaagaa atacggcttt ggcagtgccg atgcgatgca aatcatggca gaggctgaga
    61981 aatacgccta cgccgaccgc tcggaatatc ttggcgaccc ggattttgtg aaagtcccgt
    62041 ggcaggcgct gaccaataaa gcctatgcca aatctattgc cgagcaaatc gatatcaata
    62101 aagcgaaacc gtccagcgag attcgccctg gcaagcttgc gccttatgag agtaatcaaa
    62161 ctacccatta ctcagtggtg gataaagacg gtaacgccgt ggcggtgacc tatacgctga
    62221 acaccacctt cggtacgggt attgtcgcgg gcgagagcgg tattttgctc aataaccaaa
    62281 tggatgattt ctctgccaaa ccgggcgtac cgaacgttta cgggctggtg ggcggtgatg
    62341 ccaacgccgt cgggccgaac aaacgcccgc tgtcgtcgat gtcgccgact attgtggtga
    62401 aagacggtaa aacctggctg gtcaccggta gccctggcgg gagccggatc atcactacag
    62461 tgcttcaaat ggtggtgaat agcatcgatt atggcatgaa cgtcgccgaa gcgaccaatg
    62521 cgccgcgttt ccaccatcaa tggttgccgg acgagctgcg tgtcgaaaaa ggatttagcc
    62581 cggatacgct caagctgctg gaagcaaaag gtcagaaagt ggcgctgaaa gaggcgatgg
    62641 gcagtacaca aagcattatg gttgggccgg acggtgagtt gtacggcgca tccgacccgc
    62701 gctcggtgga tgatttaact gcggggtatt aaggttagcg gccctcttcg tgggaagagg
    62761 gctattttgt cagggcaagc cgaaggcagc tttttttatt tcgtaatcct gtagatattc
    62821 ttccagcggc tttgccgttc ccagagaatg aatttcacca ctgtctttat caataataaa
    62881 aggtgcgtta ccagctaagc gcgcggcctc atctccagtt tcgagaaatt ctcgtgcttc
    62941 gaaacagaaa taccagccct ggctaaagcg tccatgtaga gtaatgacga ccgggagatc
    63001 tgcatcatca aggtaatggt tcgctttcgc gaatgcgtcg tgataagtaa tcataattat
    63061 aataaatatt attgttgagt gttatattat tgttcctgtg tgatacattg agcaaaggcg
    63121 cgttcatctt cgtcaaagat attattaaat gctggttttg aaaaaagttc atcggataag
//
LOCUS       DAZFFG010000014        62540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFFG010000014 DAZFFG010000000
VERSION     DAZFFG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.014"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            213..422
                     /gene="fumD"
                     /locus_tag="ACHJ7J_001158"
     CDS             213..422
                     /gene="fumD"
                     /locus_tag="ACHJ7J_001158"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO4999425.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            878..1504
                     /locus_tag="ACHJ7J_001159"
     CDS             878..1504
                     /locus_tag="ACHJ7J_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO4999426.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLTGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1525..3627
                     /locus_tag="ACHJ7J_001160"
     CDS             1525..3627
                     /locus_tag="ACHJ7J_001160"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO4999427.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTHETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            3640..4278
                     /locus_tag="ACHJ7J_001161"
     CDS             3640..4278
                     /locus_tag="ACHJ7J_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO4999428.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4342..5010
                     /locus_tag="ACHJ7J_001162"
     CDS             4342..5010
                     /locus_tag="ACHJ7J_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4999429.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            5007..5792
                     /gene="phsC"
                     /locus_tag="ACHJ7J_001163"
     CDS             5007..5792
                     /gene="phsC"
                     /locus_tag="ACHJ7J_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO4999430.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVKYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5796..6608
                     /gene="ydhT"
                     /locus_tag="ACHJ7J_001164"
     CDS             5796..6608
                     /gene="ydhT"
                     /locus_tag="ACHJ7J_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO4999431.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(6620..8224)
                     /locus_tag="ACHJ7J_001165"
     CDS             complement(6620..8224)
                     /locus_tag="ACHJ7J_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO4999432.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAMAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEQATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYAILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQHPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARTVVKPASRARRRLSFD"
     gene            complement(8350..8655)
                     /locus_tag="ACHJ7J_001166"
     CDS             complement(8350..8655)
                     /locus_tag="ACHJ7J_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO4999433.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8763..8839)
                     /locus_tag="ACHJ7J_001167"
     tRNA            complement(8763..8839)
                     /locus_tag="ACHJ7J_001167"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8803..8805),aa:Val,seq:gac)
     gene            complement(8844..8920)
                     /locus_tag="ACHJ7J_001168"
     tRNA            complement(8844..8920)
                     /locus_tag="ACHJ7J_001168"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8884..8886),aa:Val,seq:gac)
     gene            9228..10484
                     /locus_tag="ACHJ7J_001169"
     CDS             9228..10484
                     /locus_tag="ACHJ7J_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO4999434.1"
                     /translation="MGSDAKNLISDGNVQIVKTGEVIGATQLSEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDFDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10525..11898)
                     /gene="mdtK"
                     /locus_tag="ACHJ7J_001170"
     CDS             complement(10525..11898)
                     /gene="mdtK"
                     /locus_tag="ACHJ7J_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO4999435.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            12113..12754
                     /gene="ribE"
                     /locus_tag="ACHJ7J_001171"
     CDS             12113..12754
                     /gene="ribE"
                     /locus_tag="ACHJ7J_001171"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO4999436.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12794..13942)
                     /gene="cfa"
                     /locus_tag="ACHJ7J_001172"
     CDS             complement(12794..13942)
                     /gene="cfa"
                     /locus_tag="ACHJ7J_001172"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO4999437.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(14233..15444)
                     /gene="punC"
                     /locus_tag="ACHJ7J_001173"
     CDS             complement(14233..15444)
                     /gene="punC"
                     /locus_tag="ACHJ7J_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO4999438.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            15557..16489
                     /gene="punR"
                     /locus_tag="ACHJ7J_001174"
     CDS             15557..16489
                     /gene="punR"
                     /locus_tag="ACHJ7J_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO4999439.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(16486..17511)
                     /gene="purR"
                     /locus_tag="ACHJ7J_001175"
     CDS             complement(16486..17511)
                     /gene="purR"
                     /locus_tag="ACHJ7J_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO4999440.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17810..17899
                     /gene="cydH"
                     /locus_tag="ACHJ7J_001176"
                     /gene_synonym="ynhF"
     CDS             17810..17899
                     /gene="cydH"
                     /locus_tag="ACHJ7J_001176"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO4999441.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            18065..19234
                     /locus_tag="ACHJ7J_001177"
     CDS             18065..19234
                     /locus_tag="ACHJ7J_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4999442.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19380..19961)
                     /gene="sodB"
                     /locus_tag="ACHJ7J_001178"
     CDS             complement(19380..19961)
                     /gene="sodB"
                     /locus_tag="ACHJ7J_001178"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO4999443.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGDEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(20089..20916)
                     /gene="mepH"
                     /locus_tag="ACHJ7J_001179"
     CDS             complement(20089..20916)
                     /gene="mepH"
                     /locus_tag="ACHJ7J_001179"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO4999444.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            21251..21598
                     /gene="grxD"
                     /locus_tag="ACHJ7J_001180"
     CDS             21251..21598
                     /gene="grxD"
                     /locus_tag="ACHJ7J_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO4999445.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21649..26265)
                     /locus_tag="ACHJ7J_001181"
     CDS             complement(21649..26265)
                     /locus_tag="ACHJ7J_001181"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO4999446.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAAQG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHISAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDVAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPAMISGQERLACMDDAARLRDALGVQLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGVVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLSTQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(26358..27005)
                     /gene="rnt"
                     /locus_tag="ACHJ7J_001182"
     CDS             complement(26358..27005)
                     /gene="rnt"
                     /locus_tag="ACHJ7J_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO4999447.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(27108..27515)
                     /gene="gloA"
                     /locus_tag="ACHJ7J_001183"
     CDS             complement(27108..27515)
                     /gene="gloA"
                     /locus_tag="ACHJ7J_001183"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO4999448.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(27596..28693)
                     /gene="nemA"
                     /locus_tag="ACHJ7J_001184"
     CDS             complement(27596..28693)
                     /gene="nemA"
                     /locus_tag="ACHJ7J_001184"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO4999449.1"
                     /translation="MSSEKLYSPLQVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28731..29330)
                     /gene="nemR"
                     /locus_tag="ACHJ7J_001185"
     CDS             complement(28731..29330)
                     /gene="nemR"
                     /locus_tag="ACHJ7J_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO4999450.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRNSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATSAV"
     gene            29433..29672
                     /locus_tag="ACHJ7J_001186"
     CDS             29433..29672
                     /locus_tag="ACHJ7J_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO4999451.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29721..30617
                     /locus_tag="ACHJ7J_001187"
     CDS             29721..30617
                     /locus_tag="ACHJ7J_001187"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO4999452.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30698..31219
                     /gene="sodC"
                     /locus_tag="ACHJ7J_001188"
     CDS             30698..31219
                     /gene="sodC"
                     /locus_tag="ACHJ7J_001188"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4999453.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(31220..33232)
                     /locus_tag="ACHJ7J_001189"
     CDS             complement(31220..33232)
                     /locus_tag="ACHJ7J_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4999454.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(33232..34089)
                     /locus_tag="ACHJ7J_001190"
     CDS             complement(33232..34089)
                     /locus_tag="ACHJ7J_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4999455.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVFSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(34092..34328)
                     /locus_tag="ACHJ7J_001191"
     CDS             complement(34092..34328)
                     /locus_tag="ACHJ7J_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO4999456.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            34529..34963
                     /gene="slyA"
                     /locus_tag="ACHJ7J_001192"
     CDS             34529..34963
                     /gene="slyA"
                     /locus_tag="ACHJ7J_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO4999457.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(35010..35477)
                     /gene="slyB"
                     /locus_tag="ACHJ7J_001193"
     CDS             complement(35010..35477)
                     /gene="slyB"
                     /locus_tag="ACHJ7J_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO4999458.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35751..36860
                     /gene="anmK"
                     /locus_tag="ACHJ7J_001194"
     CDS             35751..36860
                     /gene="anmK"
                     /locus_tag="ACHJ7J_001194"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO4999459.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36958..37287
                     /gene="mliC"
                     /locus_tag="ACHJ7J_001195"
     CDS             36958..37287
                     /gene="mliC"
                     /locus_tag="ACHJ7J_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO4999460.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNSRR"
     gene            37346..38002
                     /gene="pdxH"
                     /locus_tag="ACHJ7J_001196"
     CDS             37346..38002
                     /gene="pdxH"
                     /locus_tag="ACHJ7J_001196"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO4999461.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            38131..39405
                     /gene="tyrS"
                     /locus_tag="ACHJ7J_001197"
     CDS             38131..39405
                     /gene="tyrS"
                     /locus_tag="ACHJ7J_001197"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO4999462.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            39464..40327
                     /gene="pdxY"
                     /locus_tag="ACHJ7J_001198"
     CDS             39464..40327
                     /gene="pdxY"
                     /locus_tag="ACHJ7J_001198"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO4999463.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(40371..40976)
                     /gene="gstA"
                     /locus_tag="ACHJ7J_001199"
     CDS             complement(40371..40976)
                     /gene="gstA"
                     /locus_tag="ACHJ7J_001199"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO4999464.1"
                     /translation="MKLFYKPGACSLASHITPRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(41083..42585)
                     /gene="dtpA"
                     /locus_tag="ACHJ7J_001200"
     CDS             complement(41083..42585)
                     /gene="dtpA"
                     /locus_tag="ACHJ7J_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO4999465.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(43196..43831)
                     /gene="nth"
                     /locus_tag="ACHJ7J_001201"
     CDS             complement(43196..43831)
                     /gene="nth"
                     /locus_tag="ACHJ7J_001201"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO4999466.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43831..44526)
                     /gene="rsxE"
                     /locus_tag="ACHJ7J_001202"
     CDS             complement(43831..44526)
                     /gene="rsxE"
                     /locus_tag="ACHJ7J_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO4999467.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(44530..45150)
                     /gene="rsxG"
                     /locus_tag="ACHJ7J_001203"
     CDS             complement(44530..45150)
                     /gene="rsxG"
                     /locus_tag="ACHJ7J_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO4999468.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(45154..46212)
                     /gene="rsxD"
                     /locus_tag="ACHJ7J_001204"
     CDS             complement(45154..46212)
                     /gene="rsxD"
                     /locus_tag="ACHJ7J_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO4999469.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHVPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(46213..48531)
                     /gene="rsxC"
                     /locus_tag="ACHJ7J_001205"
     CDS             complement(46213..48531)
                     /gene="rsxC"
                     /locus_tag="ACHJ7J_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO4999470.1"
                     /translation="MLKLFSTFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEP
                     EEQVDPHKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(48524..49102)
                     /gene="rsxB"
                     /locus_tag="ACHJ7J_001206"
     CDS             complement(48524..49102)
                     /gene="rsxB"
                     /locus_tag="ACHJ7J_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO4999471.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGSTRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(49102..49683)
                     /gene="rsxA"
                     /locus_tag="ACHJ7J_001207"
     CDS             complement(49102..49683)
                     /gene="rsxA"
                     /locus_tag="ACHJ7J_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO4999472.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(49760..50200)
                     /locus_tag="ACHJ7J_001208"
     CDS             complement(49760..50200)
                     /locus_tag="ACHJ7J_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO4999473.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(50286..50501)
                     /gene="ydgT"
                     /locus_tag="ACHJ7J_001209"
     CDS             complement(50286..50501)
                     /gene="ydgT"
                     /locus_tag="ACHJ7J_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO4999474.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(50774..50899)
                     /gene="blr"
                     /locus_tag="ACHJ7J_001210"
     CDS             complement(50774..50899)
                     /gene="blr"
                     /locus_tag="ACHJ7J_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO4999475.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            51142..52182
                     /locus_tag="ACHJ7J_001211"
     CDS             51142..52182
                     /locus_tag="ACHJ7J_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4999476.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKSGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(52217..53218)
                     /gene="add"
                     /locus_tag="ACHJ7J_001212"
     CDS             complement(52217..53218)
                     /gene="add"
                     /locus_tag="ACHJ7J_001212"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO4999477.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLDHGIRASIN
                     TDDPGVQGVDIIHEYNVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(53322..54494)
                     /gene="malY"
                     /locus_tag="ACHJ7J_001213"
     CDS             complement(53322..54494)
                     /gene="malY"
                     /locus_tag="ACHJ7J_001213"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO4999478.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGLDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNATFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(54504..56096)
                     /gene="malX"
                     /locus_tag="ACHJ7J_001214"
     CDS             complement(54504..56096)
                     /gene="malX"
                     /locus_tag="ACHJ7J_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO4999479.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGIVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            56271..57299
                     /gene="malI"
                     /locus_tag="ACHJ7J_001215"
     CDS             56271..57299
                     /gene="malI"
                     /locus_tag="ACHJ7J_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO4999480.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            57412..58179
                     /gene="hdhA"
                     /locus_tag="ACHJ7J_001216"
     CDS             57412..58179
                     /gene="hdhA"
                     /locus_tag="ACHJ7J_001216"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO4999481.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            58408..58998
                     /gene="uidR"
                     /locus_tag="ACHJ7J_001217"
     CDS             58408..58998
                     /gene="uidR"
                     /locus_tag="ACHJ7J_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO4999482.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            59389..61200
                     /gene="uidA"
                     /locus_tag="ACHJ7J_001218"
     CDS             59389..61200
                     /gene="uidA"
                     /locus_tag="ACHJ7J_001218"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO4999483.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELYVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGKTQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            61197..>62540
                     /gene="uidB"
                     /locus_tag="ACHJ7J_001219"
     CDS             61197..>62540
                     /gene="uidB"
                     /locus_tag="ACHJ7J_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO4999484.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQ"
BASE COUNT        15752 a        15425 c        16288 g        15075 t
ORIGIN      
        1 gttacgcaaa aacttcgctg tttgcgcaac gtctggtgcc ggggttgtca ctgtgaagat
       61 aagtgaaagt tcatccggtg tataaataca tcatcgctca attgctacag ttaaaataat
      121 ttatcgcgtt atttgtctga ttttgtttga tttttgttta attggttaga ttgatatatg
      181 ctgatagttg gcatgataaa taggtataaa gaatggggaa tcggacaaaa gaggatgaac
      241 tgtaccgaga aatgtgcaga gttgtcggta aagttgtgct ggaaatgcgc gatctggggc
      301 aggaaccaaa gcatattgtt atcgcaggcg tattacgtac agcattagcc aataaacgca
      361 ttcagcgcag tgagctggaa aagcaggcga tggaaaccgt cattaacgca ctggtaaaat
      421 gaccattcag gatgaggcga taagcttcat cctgatattc ttgcttcagt tgcggtccag
      481 ttaatcattc taaataacaa ctgctgaaat tatacgaatt tcgatatgtg ttttaattta
      541 taacgataat attattttta ttttctgcaa tgaccgtcac aaataattgt cgcttaagtt
      601 aaattcattt caaatgataa taaatatcag ttcacttata atttcttgct ttgtaatttt
      661 aattgataac gatcaatgtt aagagcccag aataaccaca ttattttttc tcctaaccat
      721 aaaggattag tttatcggcg ttaaatcttt tctcatacac tatcgggtta gggaaaatat
      781 ctggtgttgg ggatagagtc agcagcgtat tagttgaatg cttaatatca ggctgctgaa
      841 ttgtcagcac tgtgtttgcg aagaggatgc gaaataaatg aacccggttg atcgtccact
      901 attagatatt ggcttaacgc gattagaatt cttgcgtata tcaggaaaag gccttactgg
      961 tctaacaatt gcccctgcgt tactgtcact tttgggttgc aaacaggaag atattgatag
     1021 cggcacagta gggttgataa atacacccaa aggggtgctg gtgacccagc gagcgcgatg
     1081 cacgggttgc caccgctgtg aaatctcttg taccaacttc aacgatggct cagtagggac
     1141 attcttctcc cgtatcaaaa tccatcgcaa ttatttcttt ggcgacaacg gagtcggctc
     1201 tggcggcggc ctgtatggcg atctcaacta taccgcggac acctgccgtc aatgcaaaga
     1261 gccgcaatgc atgaacgtct gcccgattgg tgcgattacc tggcagcaga aagaaggctg
     1321 tattaccgtc gatcataaac gttgtattgg ctgtagcgcc tgtactacgg catgcccgtg
     1381 gatgatggcc accgtaaata ccgaaagtaa aaaatcctcg aaatgtgtgt tatgcggtga
     1441 atgcgcaaac gcctgcccaa caggggcgtt aaaaattatc gagtggaaag atattactgt
     1501 gtgaatcttg caaaggaaaa aatcatggct aacggttgga caggtaatat attaagagtc
     1561 aatctcacga caggaaatat taccctcgaa gattccagta agtttaaaag ttttgtcggt
     1621 ggcatgggct tcggctacaa aattatgtat gacgaagtac cgccaggcac gaaacctttc
     1681 gatgaagcga ataaattagt ctttgctacc ggcccattaa ctggatctgg tgccccctgt
     1741 agttctcgcg taaatatcac ctcactttct acttttacca aaggaaattt agtcgtcgat
     1801 gcccatatgg ggggcttttt tgcagcgcaa atgaaattcg ctggatacga cgtcattatt
     1861 atcgaaggga aggcgaaatc accggtatgg ctgaagatta aagatgacaa agttagcctg
     1921 gaaaaagccg atttcttatg gggaaaaggg acgcgcgcaa cgacggaaga aatttgtcga
     1981 ttgaccagtc cggaaacctg tgtggcggct attggtcagg ctggggaaaa ccttgttcct
     2041 ctctctggca tgttgaatag ccgtaaccac agcggtggtg cgggaactgg cgcaataatg
     2101 ggttcgaaaa acctgaaagc gattgcggtt gaagggacga aaggggtcaa cattgccgat
     2161 cgtcaggaga tgaagcgtct caatgattac atgatgactg aacttatcgg tgcgaataac
     2221 aaccatgtcg tgccaagtac gccgcaatcg tgggcagagt attcagatcc caagtcacgc
     2281 tggacagcac gtaaagggct gttttggggc gcggctgaag gcggtccaat tgaaacgggt
     2341 gaaattccgc caggcaatca gaatacggtc ggctttcgta cctataaatc cgtttttgac
     2401 ttaggtccgg cggcagagaa atacacagta aaaatgagcg gctgccactc ttgcccgatc
     2461 cgttgtatga cccaaatgaa tattcctcgg gtaaaagagt ttggcgtgcc cagtacaggt
     2521 ggtaacactt gtgtagcaaa ctttgtccat accaccatct tcccgaacgg gccgaaagat
     2581 tttgaagata aagacgatgg tcgtgtgatt ggtaacctgg ttggtctgaa tttgttcgat
     2641 gactacggcc tgtggtgtaa ctacgggcag ctgcatcgcg actttactta ttgttacagc
     2701 aaaggtgtgt tcaagcgtgt tctgccagct gaagagtatg cggaaattcg ctgggatcaa
     2761 ctggaagcgg gtgacgttaa cttcattaaa gatttttact accgtctggc gcatcgtgtg
     2821 ggtgagctga gccacctggc tgatggttca tatgccatcg cagaacgctg gaatttgggt
     2881 gaagagtact ggggctacgc gaaaaataaa ctctggtcgc cgtttggcta tccggttcac
     2941 catgccaatg aagcgtcagc gcaggtcggt tccattgtta actgtatgtt caaccgtgac
     3001 tgcatgacgc atacccatat caactttatt ggttccggct tgccattgaa actgcaacgt
     3061 gaagtggcga aagaactttt tggttctgaa gatgcttacg atgaaaccaa aaactacacg
     3121 ccgatcaatg acgcaaaaat taaatatgct aagtggtcgc tgttgcgggt ctgtttgcat
     3181 aacgccgtca ccctgtgcaa ctgggtctgg ccaatgaccg tttcgccgct gaaaagccgt
     3241 aattaccggg gcgatctggc gcttgaagcc aaattcttca aagcgatcac tggcgaagag
     3301 atgactcagg aaaaattaga tttagctgca gagcgtatct ttacattgca tcgtgcctac
     3361 acggtgaaac tgatgcaaac caaagatatg cgtaatgaac acgatcttat ctgttcctgg
     3421 gtattcgaca aggatccgca gatcccggtc tttactgaag gtactgacaa aatggatcgt
     3481 gacgatatgc atgcttcgct cacgatgttc tacaaagaaa tgggctggga cccacagctt
     3541 ggttgcccaa cccacgaaac attgcagcgt ctggggctgg aagatattgc cgccgatctg
     3601 gcagcacaca atctactgcc tgcgtaagga atgggtaaga tgaaccatca ggatgaattg
     3661 ccgttagcga aagtcagcga agttgacgag gcgaagcggc aatggttgca ggggatgcgt
     3721 caccccgttg atacggtaac ggaaccagaa ccggctgaaa tcctggcaga gttcattcgc
     3781 cagcattctg tggctgggca gctggtggcg cgcgccgtgt ttctctcgcc accctattcg
     3841 gttgcagaag aggaactgtc agttttgctg gaaagcatta agcagaacgg agactacgcg
     3901 gatatcgctt gtatgacggg aagtcaggat gattactatt actcaacgca ggccatgagt
     3961 gaaaactacg cggcgatgtc tttgcaagtt gtggagcagg acatttgtcg cgccattgca
     4021 cacgccgttc gttttgagtg ccagacgtat cctcgtcctt acaaagtcgc catgctgatg
     4081 caagcgccat attacttcca ggaagcgcag attgaagccg cgatcgccgc gatggatgtt
     4141 gcacctgaat atgccgatat acggcaggtg gagtcatcga cggcggtgtt gtacttgttt
     4201 agcgagcgat ttatgaccta tggcaaggct tatggcttat gcgaatggtt cgaggtagag
     4261 cagtttcaga acccctgaaa tttcagggag atgtcatgga tagggtggac agttgcagcc
     4321 actgcattag gggataatca gatgtccttc actcgacgca aatttgttct ggggatggga
     4381 acagtaatct tttttactgg ttctgcgtct tctctgttag cgaacacgag gcaagaaaag
     4441 gaagttcggt atgccatgat tcatgacgag tcacggtgca atggttgtaa tatttgcgcc
     4501 cgcgcatgcc gtaaaactaa ccacgtcccc gctcagggaa gccgtttatc gatagcacat
     4561 attccagtca ccgataacga caacgaaacg cagtatcact tttttcgcca gtcatgtcag
     4621 cactgcgaag atgctccgtg catcgacgta tgcccgacag gggcgtcgtg gcgtgatgag
     4681 caggggatcg tgcgggtaga aaaatcgcag tgcatcggct gcagttattg cattggcgct
     4741 tgtccgtacc aggttcgcta ccttaaccct gtcactaaag ttgcagataa atgtgatttc
     4801 tgcgctgagt cccgactggc aaaaggcttt ccgcccattt gcgtcagcgc ctgcccggaa
     4861 cacgcattga tctttggtcg cgaagatagc ccagaaattc aggcgtggtt gcaggataat
     4921 aaatactatc aatatcagct acctggtgcc gggaaaccgc atctgtatcg ccggttcggt
     4981 caacatttga ttaaaaagga aaatgtatga acccgtcgca acatgctgaa cagtttcaga
     5041 gccagttagc gaactatgtg ccacagttca ctcccgaatt ttggccggtg tggttgatca
     5101 ttgccggagt attgctggtt gggatgtggt tggtgctggg gctgcacgcc ttgcttcgtg
     5161 ctcgtggcgt gaagaaatca gccaccgatc atggtgagag gatttatctt tacagcaaag
     5221 cggtcagatt atggcactgg tcgaatgcgt tactctttgt attgttgctg gcaagtgggc
     5281 tgataaatca ctttgcgctg gtgggcgcaa ctgcggtaaa aagtctggtt gcggtgcatg
     5341 aagtttgcgg atttttgtta ctggcatgct ggctcggctt tgttctgatc aatggcgttg
     5401 ggggtaatgg ccaccactat cgcattcgtc gtcagggttg gctggagcgg gcggcaaaac
     5461 aaacgcgatt ttatttgttt ggcattatgc agggggaaga acatcctttc ccggcaacaa
     5521 cccagtctaa atttaatccc ttacagcagg ttgcttatgt tggtgtcatg tatggattgc
     5581 tgccgttgtt actattgacg gggctgttgt gtctctatcc gcaagccgtg ggagatgtgt
     5641 ttcctggcgt aaaatactgg ttattgcagg cgcattttgc tctggcattt ataagcctct
     5701 tttttatctt cggtcatctt tatctttgca ccacggggcg tacgccacac gaaaccttta
     5761 aaagcatggt cgatggctat caccggcact aacgaatgat cattacacga gcagacctgc
     5821 gagaatggcg tatcggagcg gtgatgtatc gctggttcct gcgccatttc ccacgcggcg
     5881 gtagttatgc cgatattcat catgcactga ttgaggaagg atatacggac tgggcggaga
     5941 gtctggttga gtatgcgtgg aaaaaatggc tggctgatga aaacttcgcg catcaggaag
     6001 tttcctctat gcaaaaactg gccacagatc ccggagagag agcattttgc agtcagtttg
     6061 cccgcagcga cgatcatgct cgcatcggct gttgtgagga taacgccaga atcgcaaccg
     6121 ctgggtatgc tgcacaaatt gccagtatgg gttatagcgt gcggattgga agtgtcggct
     6181 ttaacagtca cattggcagc tccggtgagc gcgcacgggt tgctgtaacc ggcaattctt
     6241 cgcggataag cagcgctgga gatagcagtc ggattgcgaa taccggaatg cgagtacgcg
     6301 tttgtacatt gggtgaacga tgtcatgtcg ccagtaatgg tgacctggtg caaatcgcca
     6361 gctttggcgc aaacgccaga attgccaaca gcggcgacaa tgtacacatc attgccagcg
     6421 gtgaaaattc aacggtagtg agcacgggcg tggtcgactc aattattctc gggccaggag
     6481 gaagcgccgc actggcatat catgacggcg aaagagttcg tttcgccgta gccattgaag
     6541 gtgaaaacaa tattcgagcc ggagtccgct atcgtcttaa tgaacagcat cagtttgttg
     6601 agtgctgata gctttacctt taatcaaacg aaagacgccg acgagcacgg gatgcaggtt
     6661 ttacgaccgt ccgagccatc gcctcaccaa tttctgcaca tgccgtaagt ccctgaacga
     6721 aaggctggtc gtgcattaac cagggcaacg cgccgaacgc tacgcgccca cgaatatctt
     6781 cgggttgctg taacgtataa tcttcaccaa catcagggat ttcatcccct gttttctgta
     6841 attgttcgcg tagtccaggg aaaggaatat ctttcacttt aagcggatgc tgcccgcggg
     6901 catcaataaa aacgtcaaac gagtagctgt tgtcttccgt tttcaggacg gtgcgcgact
     6961 cgttaatttc cattttgtag tcttcaccga gagcgagaat atgaatgatt cccgcttcac
     7021 gtaaggccag cagccgacga atagactctg aagggattgc ggcataatta tcgatgaaaa
     7081 cccgggcaag gcctttactg aaccgtttat ggtcgtgttc attcagatgt ggaacaattt
     7141 cctgtactgc ttcatgcagg cgcagaatgg cataacgcca gggaacagta tgtttttctc
     7201 gtttattgcg ttcgacttcc tggagatttt tttctgccca gtcaaatggg tcgcgttgtt
     7261 tgcgctcggc aaaccaggct tgagcaaagg aatcgacatt caggctctct aaggctatgc
     7321 gttggctcca gtctggatca gcaaacttga tttcctctac tatcaatcta aatacccgat
     7381 ccaaaaggcc atattcgcct ttttgaatct cagcatttaa tgcctgatcg gtgacgatgt
     7441 gtaagggctc gtagggaata gggcaataga aatcggcttc gggtaaaata cccgtgcgcg
     7501 acattaacgt aatatttagc ttttcacttg cgttatcgcg gtgaaaaacg acgtgttgtt
     7561 tatcatcttc aatgaacgaa ccatgctgaa ttgccactgc cattgccgca tccagtccgc
     7621 tcaaggatgt tcccataata cccacgttac acgcatcgac ctttgcttcc atcaggcctg
     7681 accacgggct gggaaaatac gttcgggttg cttgttcttc atcaggccag acgtgacccg
     7741 tggcaatcac cgctaaatca aacgtctcgc tgggtaaatc ctgatttgta gcgagcatga
     7801 cgccagcatt tgtaatttgc agatcggtaa cctggcatga ttcataaaca gccattgcaa
     7861 atttttgctt tcgtgcctgg tctactaatc gtaaaaattg atcgcggaaa tattcgccca
     7921 gcagaattcg cggtaaaaac tgacgatcgt gcaaggtttc ttttttaacg ccataacgct
     7981 ggagatgact ggcttcttgc ttttgtagcc attcgagata cgtacaataa atcggcggta
     8041 tttcaatact ggcaatattt gccagcatca tttttgagtt ttcctcatca ctgtatggca
     8101 tcccgacacc ggcctcgtca gcctgctcga agatagaaat agaaagtgga gtttgttgct
     8161 gtagaagcga gaataaggtg tagatccccg taggcccggc acccacaata gcaatttttt
     8221 tcattgttac ttacctgtac gcattctttc gaatttagtg ttatcagata agtttggttg
     8281 agatttttag aatttgagaa ttttcagaca gggatgaaaa gggcgctaac cgcccttgat
     8341 taaattctgt caggcgagtt ttgcctgatt gatttgacta agtggctcat tgacatcgaa
     8401 aactttggcg acaacttcct caacgccgag atttttcagt cgggcagtgt gtttttccag
     8461 ataggcaagg gcgctttttt catcagtgaa aaggtagatc ccaccggctt cgtggttctt
     8521 ttcactttcg gtccatacct tccacagaaa accaggttcc tgattaatcg actcagcaag
     8581 cggtttaagc tgctcagcca ttgcgtcgcc aaatgggcca ttgaaagaaa aatgaagttg
     8641 taacaaagtt gccatagcga ttctccttct gataacgcat acgaagacag tgtaaatctt
     8701 ttaacgtatt ggatacagag aagaagcaga gggaaaaagg agaagaccag attaagaaaa
     8761 tctggtgcgt ccgagtggac tcgaaccacc gacccccacc atgtcaaggt ggtgctctaa
     8821 ccaactgagc tacggacgca ggatggtgcg ttcaattgga ctcgaaccaa cgacccccac
     8881 catgtcaagg tggtgctcta accaactgag ctatgaacgc aacgttgtag gtgacaacgg
     8941 ggacgaatat tagcggcaga gtgggaaggt ggcaagaggc aaaacgtaat tttctgcgct
     9001 atttcgaccg tttgcagagc ttttaagcaa attggctata ttttgttgat ttgcaagggc
     9061 gatttttatt caggatcgca tttacatctg atgcaaccca ataattcgtg tatcagagtt
     9121 taaacagagt tgtttgccac catctcaaat ctgtcgtgga taaatcctcc gtagccttta
     9181 cacttaggta gcaataaagg cacacagatg cagcaaagga ataaatcatg ggatctgatg
     9241 cgaaaaactt gattagcgac gggaacgtgc aaattgttaa gaccggcgag gtcattggcg
     9301 cgacgcaact tagtgaaggc gagttgattg ttgaagctgg cggaagagcc gaaaataccg
     9361 tggtcacggg ggccggctgg ttgaaagtgg caaccggtgg gatcgccaaa tgcacacagt
     9421 acggtaacaa tggcacgcta tcggtcagcg acggtgccat tgccacagat attgttcagt
     9481 ccgagggagg cgcaattagt ctctctacgc tcgctacggt taatggccgc catcccgaag
     9541 gtgaattcag cgttgataaa ggttatgcct gcggtttgtt gctggaaaat ggcggtaacc
     9601 tgcgtgtact ggaaggccat cgcgcggaaa aaattattct cgatcaagag ggcggcctgt
     9661 tggtcaatgg gacaacctca gcggtcgtgg tagatgaagg tggtgaattg ttggtgtatc
     9721 caggtgggga agccagcaat tgtgagatta atcagggcgg cgtttttatg ctggcgggga
     9781 aagccaatga tacgttgctt gctggtggca ccatgaataa tctcggtggt gaagactttg
     9841 acactattgt tgagaatgga gccatctatc gtctggggac ggatggtctt cagctctaca
     9901 gttccggtaa gacgcaaaac ctgtccgtta atgtgggtgg tcgggctgaa gtgcatgccg
     9961 gtacgctgga aaatgcggta atacaaggtg gaacagtgat cctgttgtca cccaccagcg
    10021 cggacgaaaa ttttgtcgta gaggaagatc gcgcaccggt tgaactgacc gggagtgttg
    10081 cattactgga cggcgcttca atgattattg gctatggcgc agatctgcaa caatcaacga
    10141 ttactgtaca gcagggcggt gtattgattc tcgacggcag tacggtaaaa ggtgacagtg
    10201 tcactttcag tattggtaac atcaatctga atggcggaaa actgtggctg atcactggtg
    10261 cggcaacgca tgtgcaactg aaagtgaaac gcctgcgcgg agagggagcg atttgcctgc
    10321 aaaccagtgc gaaagaaatt tcacctgact tcatcaatgt gaaaggggaa gttaccgggg
    10381 atatacgcgt tgagataaca gatgccagtc ggcaaactct gtgtaatgca ctgaaactac
    10441 agccagacga agacgggatt ggcgcaacgc tccagcctgc gtaaaaatac aggcgcaacc
    10501 cgtgaaggtt gcgccttgtc tttattagcg ggacgctcgt tgcagaatga tggctgacgg
    10561 catacgttgc aggaaccgca tacgcagcat catcataatg gctgccgacg tcaggccaat
    10621 aataaagcct atccagaagc ctgctggccc cataggttca acgaccagat cggtcagtgc
    10681 cagaatatag ccgcttggca agcccagcac ccagtaagcc gtaaaggtga tatagaaaat
    10741 ggaacgcgta tctttataac cacgcaaaat cccactgcca atcacctgga ttgagtcaga
    10801 aatctgatat accgccgcca gcaacatcaa atgcgcagcc agcgttacaa cctcgggatt
    10861 gtcgttgtac aacagggcga tttgctcccg cagtgaaacc gtgaatatgg ccgtcagggt
    10921 tgccatacag acacccacca taagcccggt ccgcgcagcg gtttgcgcat ccagcgttga
    10981 gccctgaccc agacgataac ctacgcggat agttaccgct gccgccagcg acattggaag
    11041 cacgaacatt agtgaactaa agttcagggc aatctggtgt cctgcgacat caacaatacc
    11101 gagcggagac actaacagag ccacgacggc aaacagtgtc acttcaaaga atagtgccag
    11161 cgcaatcggc aaaccgagtt gaatcagtcg tttcataacc gcaggatcgg gtttcgcggt
    11221 gcctttttcg ttacgaatat cgcgcatgga gcgggcgcgt ttaatgtaag aaaccatggc
    11281 aaggaacatg acccaataca ccgccgcagt agccacgcca caaccaacgc caccgagctc
    11341 aggcataccg aaatgaccat aaataaagat atagttcacc gggatgttca ccagcaggcc
    11401 gataaagccc attaccatac ccggcttggt ttttgccaga ccttcacact ggttacgggc
    11461 aacctggaag aacagatatc ccggcgcgcc ccacaacaac gcacgcagat aacccacggc
    11521 tttgtccgcc agagccggat cgatgttttc catggagcgg ataatgtaac ctgcattcca
    11581 cagcaccagc ataatgagaa cggaaacaaa acctgccagc cagaaacctt gtcgcacctg
    11641 atgcgcaatg cgctcacgtc gaccggaacc atttaattgc gcgataaccg gcgttaatgc
    11701 cagcagcagt ccgtgaccaa agaggatcgc cggaagccag atagaagtac cgatagcgac
    11761 cgccgccatg tcggtggcac tgtagccgcc cgccatcacg gtatcgacaa aacccatcgc
    11821 agtttgggcg atttgcgcga gaatcaccgg gattgctaat gctaataaca gacgcgcttc
    11881 actgatatac ttctgcacgt gaacaccttt tatttgtagt tatatgagag actaaaaagc
    11941 cgcctgaatg ggcagcaaaa agaagaagca ggggaaattc agtctattgt agcgaggatt
    12001 tattaattct ccagtgaaaa aatagtcatt cttacccctt cactggtaag ggcgtttgcc
    12061 acctgctatt gtgctgaaca gaatgtctta actgatttca ggagttgtaa gtatgtttac
    12121 ggggattgta cagggcaccg caaaactggt gtcgattgac gagaaaccaa attttcgtac
    12181 gcatgtggtg gagttacccg accacatgct ggacggcctg gaaaccggtg cgtccgtggc
    12241 gcataacggt tgctgcctga ccgtgacgga aattaacggc aaccatgtca gttttgacct
    12301 gatgaaagaa acgttacgca ttaccaatct tggcgattta aaagtggggg attgggtaaa
    12361 cgttgagcgt gcggcgaaat tcagtgatga aattggcgga cacttaatgt caggtcatat
    12421 tatgaccacc gctgaagtgg cgaaaatatt aacctcggaa aataatcgcc agatctggtt
    12481 taaagtccag gatagtcagt tgatgaaata tatcctgtac aaaggattta ttggcatcga
    12541 cggtattagc ctgaccgtcg gcgaagtcac gccaacgcgt ttttgcgtcc atttaattcc
    12601 ggaaacactg gaacgcacga ctcttgggaa gaaaaaactt ggcgcacgcg tcaacattga
    12661 aatcgatcca caaactcagg cagtggtaga tacggtagaa cgtgtgctgg cggcacgaga
    12721 aaatgccatg aatcaaccag gtacagaagc ctgatctgaa atcgcccccg gagcgggggc
    12781 gatagaatag cctttagcga gcgactcgaa ggccgttttc aacaccgcgt gagaacacca
    12841 cctgccagag ctgaatatca cgggcgcgga aagcacctgc gcaggcattc agataatagg
    12901 taaacattcg tttaaagcgt tcactgtagt tatccgcaat ttctggccat gcggcgagga
    12961 atcgttcata ccacgccatc aacgtagtat cgtaatcagc accgaagtta tgccagtctt
    13021 ccatcacaaa gtggggttcg ctggattgag caatctggcg tacagagggc aggcaaccgt
    13081 tcggaaaaat atatttgtta atccagggat caacattcag atcggttttt ttcgaaccga
    13141 tagtatggag caggaatata ccttccggtt tcaaattacg atccaccacc gcaaaatagg
    13201 tatcgtaatt tttcggtccg acgtgctcga acatccccac agaaacaata cgatcaaact
    13261 ggtcgttcag gtcacgataa tcttgcagca aaatggtgac atccaggcct tcacagcgtt
    13321 cctgagccat tttttgctgt tcggcagaaa tggtgacgcc aaccacgctt acgtcataat
    13381 tagacgccat gtagtgcgcc agtccgcccc agccgcagcc aatatccagt acgcgcatcc
    13441 ctggttttaa ctgcaatttt tcacaaatca ttttgagctt cgcctgctgg gcagattcca
    13501 gattatcggc atctttccag taagcgcagg aatattgcat gaagggatca agcatgcggc
    13561 tgaacaagtc attacccaaa tcataatgct ctttgccgac tatccaggca cgttttttac
    13621 tctgcagatt gaagagacga gcgccggcaa tacgcagcgt gtctttgaaa tgatggggga
    13681 gttggttctc gagacctgcg cgtaagactt tgctaaaaaa catatccagt cggtcacatt
    13741 cccaccagcc atccatataa ctttcgccta accccaaaga gccttcttgc agaacgcgtt
    13801 taaaaaaatc ggggtttttc acacgaatat ccgccggggc agaaccgtta atggctatac
    13861 cggcacggct aagtaattcg ttggcgatac ggtaccagtt gtcatccggt acactgactt
    13921 cttctataca cgatgaactc atagtttctc catcactggt cggtgatcag aaccttaaaa
    13981 cagcgtagac gctttttggg ctttgtgaga aatctcgcag aaaaaaccgc gagtctataa
    14041 tccgacgtag aacagaggga aaggaggaac ccttgccgaa atgaccatcc atagtgagta
    14101 gggaacgatc ctgtccccgt aaaaacataa tatttatcgt agctttaaca actcgaaatt
    14161 agtataggcc gctaatttca atcatacaac aaaattttgt tagcagccta agtataagta
    14221 tatcgatata gatcagtgtg attcgctatg agcgacttcg gcattgccat gattctggca
    14281 gccaacttct tcacaacgtt gcatcatgta acccagcgcg accagcacta ctgttgataa
    14341 catcacgctg gtggtggtga gcaatggcgt gctgatactg atcagccagg aaactaccag
    14401 acttgcgagg aagcacagac ccagttgtag agtgttctgc aacgctgcgg cgcgaccagt
    14461 tgcgtgtggg aagggacgca gcgcctgggc gacaacaata gggtagatcg cgccattggc
    14521 aatcgccatc acacagaatg ggatcaggat ttcgaccagc gacacatggc taatgaagcc
    14581 cgcagcccag gtcgcaatga cgctgacagc aaacagcacc agcaaccacg gtaataactg
    14641 cttgccttgc catttctgca gcgcggcgcg acagccataa ccaccaatca gaaacgcgat
    14701 agtttgcgga acataactta aaccaataac tgccgggctg tagcccattt cactaaggat
    14761 gaacggtgaa ccggtcagcc atgcaaaaaa actggctgaa caggccgcgt atatcagcac
    14821 gttgccgcga taggttttag aacgtagcag gtcggtaaag gtcagaccat cctgactatt
    14881 gttacgggcc ttcgtcgtgg gtttgagcca gaaaataggc agaatcagca ccacggtaat
    14941 ggcaaaaagg gtggcgaaaa tcgcctgcca ggaaaaatgg accagcagcc agcttcctaa
    15001 cagaggagcc agggccggag atagacccac cagcggcatg atggtcgcaa aaattcggtt
    15061 aactttctgt gaaggataat aatccgtcac taacgcttgc cagataaccg ccgcggcgca
    15121 gacacccaca gcttgtacaa aacgcaatac cagcagcata gcggcgtttt ctacccacag
    15181 catccccaga ctacctaacg caaaaattgt caggccgatt aataataccg gtttacgacc
    15241 ataacggtcg gaaagcggcc cccacagaag ctgggctgcg gcaaaaccgg caaggaacag
    15301 actaaggctg gcactaacag cagacgcagg cgtttgcaga tcggcctgta tggcggcgaa
    15361 agcaggcagg tacatatcgg ttgccagaaa accgagtacg ctcaaacccg ccagccagac
    15421 taaaaatctt ttcccaggtt gcatgtatat tctctaaatc tctcaattaa acaccgccgc
    15481 aaagtgtagg gagtgaaatt agtgttgtga aacggtaata tttgatgact ggtttcaaaa
    15541 attttgcagg cagactatgt ggtcagaata ttcactcgaa gttgttgatg cggtagcgcg
    15601 taatggtagt tttagtgccg cagcccagga gttgcatcgc gttccttctg cggtcagcta
    15661 taccgtacgt cagctggaag agtggctggc ggtgccgctg tttgaacgac gtcaccgtga
    15721 tgtggaactg accgctgccg gggcgtggtt tctcaaagaa ggtcgctctg ttgtcaaaaa
    15781 aatgcagatc acccgccagc aatgtcagca gatagcgaac ggctggcgtg gtcagttagc
    15841 tattgcagtg gataatattg tcaggccaga acgtacacgg cagatgatcg ttgattttta
    15901 tcgccatttt gatgatgtcg aacttcttgt ctttcaggaa gtgttcaacg gtgtctggga
    15961 tgcgctttcc gacgggcgcg tggaactggc gattggcgca acacgcgcga ttccggtagg
    16021 cggtcgttat gccttccggg atatggggat gctaagctgg agttgcgttg ttgccagcca
    16081 tcacccgctg gcgttgatgg atggcccgtt cagcgatgat acgttgcgca actggccgtc
    16141 gttggtgcgc gaagacacct cgcgaacgct acctaagcgt attacctggt tgctggataa
    16201 ccaaaaaaga cttgtcgtgc ctgactggga atcatcggca acctgtatct cggcaggatt
    16261 atgcataggg atggtgccaa cacatttcgc caaaccgtgg cttaatgagg ggaagtgggt
    16321 agcattagaa ctggaaaacc cctttccgga ttcggcatgt tgtctgacat ggcagcaaaa
    16381 tgatatgtcg cctgcgttaa cctggttgct tgagtatctc ggcgatagtg agacgctgaa
    16441 taaggagtgg ctgcgggagc cggaagagac tcccgcaacg ggtgattaac gacgatagtc
    16501 gcggaacggg ccgtcagcca cggagcggcg ttcaatcaag cgcggatgca cttcaatgga
    16561 ctgcggttct tcacgtttgt tgacgatacg atccaacagc atgttgaacg ctgtttcacc
    16621 cagcgagtct tttggctgat ggatcgtggt cagcgccggc gtaaaatagc gggcgttgcg
    16681 cacgttatca taaccgatca gcgaaacatc ctgcgggacg cgcagcccca tttcatcagc
    16741 agcacaaagt gcgcccattg ccatgatatc gccaccacag aagacggcag tagggcgatg
    16801 cggctgcgac aggatttgct gcatggcgcg ataaccggat tcaggttcaa aatcaccctg
    16861 cacaatccag ctttccggca ccttgatcat cgcttcttcc atcgccttca taaaaccggc
    16921 aaggcggcct gcgccggtgt tacgttccag cgggccgggg ataacgccga tttcgcgatg
    16981 gccgcgttct atcagataac gcccggccat gtagccgcct tcgaacgcgt tatcaatgac
    17041 cgcatcggtg aagtcagctt ttgcttcacc ccagtccatc accaccattg ggatatggcg
    17101 atactcttcc agcatcgcca gcaacggctc tgggtactca gaacacatta ccagcagacc
    17161 atcgacgcgt ttttgcgcca tcatcgacag ataagcccgc tgtttctcaa gattattcca
    17221 cgcattgccc agaatcaggg tgtaaccttt ctggaagcaa tttttttcaa ctgcttcaat
    17281 gatctcggca aaataggccg cttcgctgct ggtcgccagc aaaccgatag acttggtgtg
    17341 gttaaccttc aggctacgcg ccaccgcgct aggggagtag tgtaattctt taatcgctgc
    17401 ccacacggcg ttgcgcgttt cttcagcgac gaaacgtgtt ttgttgatca cgtgtgacac
    17461 agttgtagtg gaaacgtttg ctcgtttcgc tacatctttt attgttgcca ttccatttca
    17521 ctccagaccc taacttcagc tcctgcaaaa tcgcaaggta aacgtttgcc tttacacacc
    17581 ttacgcacaa caaaagtgtt gcggtacgcc ggaaaaacga cacggagcgt caggaagggg
    17641 tcaatggcca gtacgcaaat agtgtaagcg tggaattttg gccgatcttg acgaaaaggg
    17701 gaagtggtaa aaacacttat cattataaat gacgggagat tttttcatca cagtgtgtaa
    17761 aaatgcgtaa tatcactttt tgtgtggtaa taaaaaagga gaaaacttga tgagcaccga
    17821 ccttaaattt tcactggtaa caacgattat cgtcctcggt ttgatcgtag ccgtgggttt
    17881 gactgccgcg ctgcactgat ttcctctcga gggagcgcgt tctccctcgc atctccttcc
    17941 tgtttgttag caaaatggca aaagtgtttt ctctttttgt tattttcatt tttttgtgat
    18001 tgctgtcatg ctttcgccac tttaatgaat atcgcgtaat gacgcgaaga ggtggagata
    18061 aggcatgaaa attaactatc cgttgctggc gctggcgatt ggcgcgtttg gtatcgggac
    18121 aacggagttc tcgccaatgg gcttgttgcc cgtcattgcg cgcggtgtgg atgtctcgat
    18181 tcccgctgcc ggaatgttaa tcagtgccta tgcagttggc gtaatggttg gcgcgccgct
    18241 gatgacgctt ctactttctc atcgtgcccg ccgcagtgcg ttgattttcc tgatggcaat
    18301 tttcacgctc ggcaacgtac tttccgccat cgcgccggat tatatgaccc tgatgctttc
    18361 acgcattttg accagcctga atcatggagc attttttggt ttgggttcag tcgtggctgc
    18421 aagcgttgtg ccaaaacata aacaggccag cgcagttgcc actatgttta tggggttaac
    18481 cctggcaaat atcggtggcg tgccggcggc gacctggttg ggtgaaacca tcggctggcg
    18541 gatgtcattt ctggcaacgg cggggctggg agtgatttca atggtaagtc tgttcttctc
    18601 attacctaaa ggtggtgcag gggcacgacc tgaagtgaaa aaagagctgg cggtattaat
    18661 gcgtccgcag gtgctgtctg cattgctgac gacggtactg ggagctggtg caatgtttac
    18721 tctctacacc tatatctctc cggtactgca aagtattacc cacgcaacac cggtgttcgt
    18781 cacggcaatg ctggtgctga ttggtgtcgg attctctatc ggtaactatc tcggcggcaa
    18841 actggcagat cgttcagtta acggcacgtt gaaaggcttt ttgttgctgc tgatggtgat
    18901 tatgctggca atcccgttcc tggcccgcaa tgagttcggc gcagctatta gcatggtggt
    18961 gtggggcgct gcaacctttg cggtcgtacc gccgttacag atgcgcgtga tgcgtgtcgc
    19021 cagtgaagcg ccaggtctgt cttcatcagt caatattggt gcctttaatc ttggaaatgc
    19081 gctgggagca gccgctggtg gtgcggtaat ttccgctggg ctgggataca gctttgtgcc
    19141 ggtgatgggg gcgattgtcg cgggactggc attattgctg gtgtttatgt cagccagaaa
    19201 acaacctgaa acagtttgcg ttgctaacag ctaattaaac aaaaacgcag aaggataacc
    19261 ttctgcgtta cagttgctga caacgtgcga tttatcttgc cgaatgcgct gttaacgcct
    19321 catccgcgtt ggcatggtaa gcgaagcgca tcaggcaatg ctgcatttgc catcagttat
    19381 tatgcagcga gatttttcgc tacgaattcc cagttcacca gcgcccagaa gtgctccaga
    19441 tagccaggac gtgcattgcg ataatcgatg taatatgcgt gttcccagac atcaacggtc
    19501 agcagcggag tcgcatcggt ggtcagtgga gtacccgcgt tagaggttga aacgatagcc
    19561 agtttgccat cgctgttttt caccagccag gtccagccag aaccaaagtt tttgatcgct
    19621 gcatcagtaa actgagcttt gaaatcggca aagctgccaa aagatgcggc gatagcttca
    19681 gcgacttttc cagtcggttc gtcaccggcg ttcggtgcca ggcagttcca gtagaaagta
    19741 tggttccaga cctgagctgc gttgttgaat acgccacctt cagagctgcg aataatctct
    19801 tccagtgatt taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt agtgacataa
    19861 gtctgatgat gcttgccgta gtgatactcg atagtttccg cagaaatgtg cggtgccaga
    19921 gcatctttag catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat
    19981 taatttgcat acgtacaata gccttattgt gcgtatggat agggtagcaa attaaaaggt
    20041 gagacaaaag atgaacttac cctgttgccg taacaacagg gtaaagtttt agcgaagtgt
    20101 ttttggggtc attacccggc gagcgccaac atagtggcgc tgccagtagt cttcactgag
    20161 agaagtgatt tgaatttcct gacccgtacg cggtgactga ataaacttgc cgttgccgac
    20221 atatacgccg acatgatcgg ctgtaccgcg tccctgagta cggaaaaaga ccaggtcgcc
    20281 gtttttcagt tcactacgtt cgattggcgc tgcatcacgc aggtgataca tttcattcgc
    20341 cgtacgcgga atacgaattt tcaccaaatc tttataagcg taataaacca ggccgctgca
    20401 atcaaaaccg gtacgcggtg agctgccacc ccaacgatat ggcttaccaa tttgctgcat
    20461 cagtttattc attgccactt ttgtggcttt ttgcaccttc gctttgtgtg cgtcggcaag
    20521 ggttcctgac gctgcatttt tgactttcac acaatgcgat ttatagccct tacgtttggt
    20581 acacttttcg gtaaagctgg ctgaggccgt tttattgacg gtatgtttgc tgcgagtttt
    20641 ggcgttttta gaagcggtaa tggaagattt tttaacggaa gaagcggttt tgcttttggt
    20701 cgtactttta ctggcggctt ttttcgctgt tttctggctg gctttctggg tctttttggt
    20761 ggttgccgtg cttttctttt tatctgcttt tttggtgata tgagtggtag cagaactctc
    20821 cctggcttgc tttgaagcat gggccatagg cgttaaaggc agggtggtaa aaagtaaagc
    20881 acagagcgtg atcgaaatac ggtttatccg cgccactgag cgtccctctg ttaaggcagg
    20941 tctatcatgc ctgcgtctga tttacaaaac ttgatgattc taatgcatag aagaagagaa
    21001 agttaatagc ttttattgtt aaaaaatacg tttcgttggc gagcgcaaaa aaatgcagca
    21061 gtgaaagcta aaaatggtca taaatacgcc agtttgccgt gcatcaggga agaaacgaac
    21121 atttgaaatg caacaaatag agcaaaggga taaaatagca aaagcgtgat gaaaatgtta
    21181 ttttccgatg ccgcactgac ccgctacaat gtcagattac tgccgtaaag aagtttgagg
    21241 aagcaagaca atgagcacca ctatcgaaaa aatccaacgc cagattgctg aaaacccgat
    21301 cctgctgtac atgaaaggtt caccgaaact gccgagctgc ggtttctctg cccaggcagt
    21361 ccaggcgctt gccgcatgtg gcgaacgttt tgcctatgtt gatattctgc agaatccgga
    21421 cattcgtgcg gaactgccga aatatgctaa ctggccgacc ttcccgcaac tgtgggttga
    21481 cggcgagctg gtaggcggtt gtgatatcgt gatcgaaatg tatcagcgtg gtgaactgca
    21541 gcagctgatc aaagaaactg ccgctaaata caagtctgaa gagccggacg cggaataatc
    21601 cgtcctgcaa tactgacgga tgcgaacggg catccgtcag tcctttctct atccccaatc
    21661 cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc agcgtaaaca tcggcgtttg
    21721 ccggaccggt agatcattca cttctgttag cgtaaagcgc agccgtggtt cgcgacgcag
    21781 tgcggtagtc agcgcgtgga aaacctctgg ggcgagtaat tcctcttttt cctgccagac
    21841 cagcattttt ttgccacctt gcgccagata gagtaacaat ttgccgccag aaacgaccac
    21901 cagcgcaccc gcccgacgcg ttggtaccag cgttgccgga tgtgcaggcc agggcagaag
    21961 atttccccac acattagccg gatcgttggc ggagagcgcc actggtgtat agtggcgcgt
    22021 ttgcgcggct tgtgtcgaca ggtcgcgcaa gcggtcaata gtcagacgtt cagcgaattg
    22081 cgcgccaccc agaccttcta caaaacgacc tcgcataatt cgcccggaat cttccatact
    22141 tcgacaaagc gtttgcatcg atggaaaccc gccagggata ttttcggcta tcaccgcctg
    22201 acgactgatg atgccgtagc ggtcgagcat attttccgcc agcgccagca tcctttcggt
    22261 atcgtttagt ggctccacct gcaataacga ccagcgtcca gccagatttg gtgtgttgta
    22321 agatacccgc ggcgagacgg gttgtgcata gacaggacgc ccacgacgag cccggtgact
    22381 gcggcgagtt gaggtgcgtg cgttggaact gctgcgggtg agggcgcgta acggtgccca
    22441 aatatcgctg gtgatgacgc cttgccagac cagcgcccat aacgcctctt gcagtgccga
    22501 gggatcaacc gattcgccga ttttgtcgcg tatccgttgg ctgatttgtt gtgcaaacca
    22561 ggctcctccg tcagccagaa cagcgactat cgcttgctgc agcgccgaac gattcgcctg
    22621 atccgcttcg gcgggagtga acgattctgc ggcatattcc tgtagatgca gtgccaccag
    22681 gccgtcatct tcacccagct ttttttgtcc cgaccagata accactccgg ttgccagtaa
    22741 ttcatcgagc atttctggcg aatagtcgcg tacgcgggca ggcaggatct ggctttccca
    22801 gagtgaggcg ggtaaaccga ctccggcaag ctgttcgatc acccgcatca cgccatctac
    22861 gccctcataa acgcctggcg atgttgaggc aaagagcgcc gggctaccat cggtggcggg
    22921 taatacgccc tgacgttcca gcagcaatcg cgcataggtc gtggctgcaa cgggacgcgt
    22981 cgcttctctg gcggcttgca gcgagcgcaa acgcagacga cgaaatactt catcgctcac
    23041 ccagatgtct tgttgcagat tcatcactag accctgttcg cgcagttgct gaagctgctc
    23101 ttcgacaatg gcaataccga gactaaactc atgagccagt tgttcagccg tgaccagagc
    23161 atgagcccgg agatagcgca gaaagaggtc gcgaagcggg taactgactc tatgcaaata
    23221 aatctctggc agagactctg gtagttgtac gccgagggca tcgcgcagcc tggcggcatc
    23281 atccatacag gcaagacgct cctgtccgct aatcatcgcc gggaagattc gttttactgt
    23341 aagaagattt tccagatacg acgcaatctc ttcactgctg cctgtatgcc gttgcgccag
    23401 gtcttcaacg gtcattggcc ccagttcgcg cagcaggtcg aacaatcctt cttcaccttt
    23461 cgctcttctg ccaggagcca gtcgttgcaa ctcttcttcc acctggcgaa tgacctgcgg
    23521 gtcgagtaat tcccccggat cgacctgtcc gagtagattg cgcagtaact cgctgtccag
    23581 cgacagtacg gatgcccggc gctctgccag cggggcgtcg ctctggtaca taaattccgc
    23641 gacatagccg aacaataaac ttgtggcgaa aggcgagggc gtagtagtcg ttacatcgga
    23701 tatttgaatt tcgccaccgt tcaggcgacg catcaaacgt tccagtgcgg gaagatcata
    23761 aacatcttgc agacattcgc gtagggtttc gagaatgacc ggaaaatccg gatatccctg
    23821 agcgatttcc agcaactgac tggcgcgcag ccgttgttgc cagagcgggg tgcgatggcc
    23881 cggcgtgcgg cccggcatta ataacgcccg cgcggcgcat tcgcgaaaac gggcggcgaa
    23941 aagtgctgag ctgcctaccg cctcgcggac aatttgcagc aacttttctg gttcaaacaa
    24001 aaaaatcgcg gcgtcgggca atttaccatc agtgtcagga atgcgcgcaa caatgccgtc
    24061 atcactggcg accacagacg catcagcgcc ccatagcgca tgtattcgcc cggcaatcgc
    24121 cagcgcccag ggttcatgca cccgtcttcc atagggagag tgcaaaataa tacgccagtc
    24181 gccaatttca tcacggcacc gttcgaggac cagatgacga ctccccggaa cgatgcccgt
    24241 cgcgttgcgc tgctcgtcaa tcaacccctg aatattggcg atcgtatttt cttcagccag
    24301 ccacggggga atagtcccgg aaaagaacgc gccatccgcc agtaaatgga gaaaatcgcc
    24361 gatcatctcg cctaattcag ccggacgtcc gttaccttca ccacgccaaa agggaagccg
    24421 ggcagaacga cccggagcag gagtcacaat cacctgatcg cgggtgattt gctggatccg
    24481 ccatgaggta gcgccgagag tgataatgtc gttcacccgc gactcatata ccatctcctc
    24541 atccagttca cccacccgcc gcgaaccggc ctgttcttca ccttcgggta ataacacgct
    24601 atacatgcca cgatccggaa tggtgccacc gctggtaacc gccaacagct gagcgccagg
    24661 tcgggcggtc aatatcccgg tctcccggtt ccagaccagt tttgggcgaa aagcagaaaa
    24721 atcgccagag ggatagcgcc cggaaagcat atccagcgtg gcgtcaaaaa cacgtcttgg
    24781 cagatctttc cacggtgcgg cacggcgtac gcgggagtac cattcgtcta cctgtaatgc
    24841 atccatcgcc gcggcggcaa cggtgtgctg cgcaaggacg tcgagtggat tatgcggtgg
    24901 tgtcaggttt tccagcctgc ctgcgaacat acactctaca atgactgcgg aatcgactaa
    24961 gtcacgccgg gttcggggga aaaacagccc tttggagata cctcctacct ggtgtccggc
    25021 acgtccaatg cgttgtaacc cactggcaac agaaagcggc gttgccacct gaatcaccag
    25081 atccaccgcg cccatatcaa tcccaagttc gagactggaa gttgcgacca cgcagcgtaa
    25141 ttcacccgat ttcagcgcct gttcggtgat tgctcgttgt tctttagaga cggagccgtg
    25201 gtgtgagcgg gcaataaaaa cgtcgctact ttgtacacgg ttagaggttg cgccggaggt
    25261 cgactcgaaa tgggccacat caacggcgat agaaggggaa cgctgtaagc gtgcggcgta
    25321 aagctcattt aatcgtgccg tcagtttttc cgccagcccc cgcgaattag taaagacaat
    25381 ggtcgagcga tggcgcaaca cttcatcaag gatacccgtt tcaatatatg gccagatgga
    25441 gccttcccgg ccggcatggc tgtcttcgcc agtgccgctg gcgaccgatg agacatcatc
    25501 catattggcg accggtacga caatccgtat ctgcggatgg cgcattgcgg gcgggttgac
    25561 taccgtaacc gggcgatcgc caccaagaaa tgctgccaca tcgctggcgg agcgcacagt
    25621 ggcagaaagg ccaattcgct gtgctgagat gtggagcagc gcatcgagcc gctccagact
    25681 taacgccaga tgcgcaccac gtttactgcc cgctaccgcg tggacttcat caataattac
    25741 cgtttcgacg ccgcgtagcg tttcgcgcgc gcgggaggtc agcatcagat agagtgattc
    25801 gggtgtagtg atcaaaatat ccggcggatt acgggtgagt ttgctgcgtt cctgtgcagg
    25861 cgtatcgcca gtacggatcc ccacgcgaag attgacttcc gtttcgccgc gccgccgccg
    25921 ttcatcggca ataccctgta acgggatctg caagttgcgc tgaacgtcgg tgcccagggc
    25981 ttttatcggt gaaatataga ggatgcgtga ggttttacgt ttatgcgcct cgcgggtatc
    26041 ttcgccgcct tcgcggaaga gctgatcgag ggcgtagaga aatgctgcca gcgttttccc
    26101 ggagccagtc ggcgcaatca ccagcgcatg ttcgccttgc gccgccacat gccaggtttg
    26161 cgactggaca gcggtcggct gtttaaaggc gcgaagaaac cagtcgcggg tcgccggtga
    26221 aaacacgtcc ggcaggggcg atgaagggtt tggattatct gccattaacg ctattccccg
    26281 tgaatatcat gcatgatgcg tggaatagcc tcactataga tgcctgaatc attgcatgtc
    26341 atcaggcatc gactcgatta cacctcttcg gcggcaggta gcggccagcc tcccagacgt
    26401 ttccagcggt tgacgatttc acaaaacagc accgcagtgc gttcggtgtc gtacagcgcg
    26461 gagtgagctt gggtgctgtc gaagtccatg ccagcggtct ggcaagcctt tgacaatacg
    26521 gtttgtccga gtgccagccc ggccagtgca gcagtgtcaa aagtggcgaa agggtggaac
    26581 gggttacgtt tcagtgaggc gcgttcagcg gcggccatca taaagctgtg atcgaaattg
    26641 gcgttgtgcg ccaccataat ggcgcggtta cagccgctcg ctttaatacc tttacgtaca
    26701 actttaaaaa tttcgtgcag cgcctcgtat tcgctgaccg cgccgcgatc gggatcgttc
    26761 gggtcaatgc cgttgaaggc aagggcttct ggttgcaaat ttgcgccgac aaatggttcg
    26821 acgtggaaat gtaatgtggt gtccggcatc agccagcctt gttcatccat tttcagggtg
    26881 atggcggcaa tctcaagcag cgcatcggtt ttggcgttaa atccggctgt ttcaacatcg
    26941 atcacaacag gataaaaacc acgaaaacgg tcgcacagac cggtaagttg agcgttatcg
    27001 gacatctgat tctcttaata cggataaatt gcagcgcgca ttatgacaaa tattacttgt
    27061 cgatgcagta aagatgcggg cgcgatgagt tcgcgcccgg caggagatta gttgcccaga
    27121 ccgcgaccgg cgtctttctc ttcgattaac tcaattttgt aaccgtccgg atcttccaca
    27181 aacgcgataa ccgtggtacc gccttttacc ggacccgctt cacgggtcac gttacccccg
    27241 ttttgacgga ttttttcgca cgcttcagcg gcattatcta cgctaagcgc gatgtgacca
    27301 taagcagtgc cgagttcgta tttatccacg ccccagttgt aggtcagttc aatcaccgct
    27361 tcttcggttt ccgggccgta gccaacaaac gccagtgagt atttgtattc cgggttttcg
    27421 ctggtacgca gcagtttcat gcccagaact ttggtataaa aatcgatgga gcgttgcaaa
    27481 tcgccaacgc gcagcatggt atgaagaaga cgcatctttt tatcctcaaa atggctaaca
    27541 gattcaaaat gttgttttag tatagcggcg gctttgcgcc gctcgcaatg ttggattaca
    27601 acgtcgggta atcagtgtag ccttccgcac cgccaccata gaaactttca gcacgctgtg
    27661 ggttaagctc agctttgcgc tgcaagcggg cgaccagatc cgggttcgca atccagtcac
    27721 gaccaaatgc caccgcatca attaaccctt tgccaatcag cgtttcggct ttttctaccg
    27781 tgtatgcacc tgcgccgata atcggaccgt ggaaacgggc gcgtactttt tcgcggaacg
    27841 catcagtata cggttcacct cccgcccaat ctggttctga catatgcaga taagcaatgc
    27901 cgcgtttacc cagttgttca atcagataca gtgcatcggc ttcttcattc gggccgttat
    27961 ctgtgttctg gaaagtaccg attggcgaaa cgcgaatgcc aatgcgatcg gcaccccatt
    28021 cttcaatccc ggcatcgacc acttctagta ccaaacgtgc gcgattttcc acgctgccgc
    28081 cgtactgatc ggtacgatgg tttgaagaag gggaaaggaa ctgatgcagc aaataaccgt
    28141 gagcagagtg gagctctacc agatcaaaac cggcttcacg cgcgttagca atggcctgac
    28201 ggaaatcatt gacgatacct ggaatctctt ccagttcaag cgcacgcggc atggatgttt
    28261 caacacggat cgcctgacca ttttcatcgc gcagagaagt acgtgttccc gcgctaagtg
    28321 ctgaaggcgc taccggtgcc tgaccgccag gttgcaggct ggcgtgagaa atgcgtccgg
    28381 tgtgccacag ctgtacggcc atatgaccat tttcagcatg aacgccagcg gtaatttttt
    28441 tccatgcggc aatttgttcc ggactatgga tgccaggtgc accagcatac ccttttgcct
    28501 gggcagaaat ttgcgtggct tcgctaataa tcaaaccggc actggcacgt tggcgatagt
    28561 attccgccat caacggggta gggatgtcac ccggttcaat actgcgcaga cgcgtcagcg
    28621 gtgccataaa aatacggttt gccgccgtga tcgcgcccac ttgcagtggg gaatacagtt
    28681 tttcagatga cataaaggct cccggtcgtc tggtgataaa aaaatgcctg ctaaacggca
    28741 gacgtcgcaa taatggtttt tacatgtgcc agcgcgtttt caagtggctc gaaactgcgc
    28801 gaaattttgg cctgcagatt tgcgccgagc cacagcgcgt aaagcacttg cgcctgttgt
    28861 agcggttcgc cgcaaaaggt taaacaatgg ctattacgac cattttccag cgcctgagag
    28921 agcagggcga tcacgccgcg agcaccttta tccattgcgc tgcgcatatc ttctgacaga
    28981 tcgcacactt cggcagagag ttttactgtc aggcaaccac tgatggttcc atgttggcaa
    29041 aactggttca gtgtttgctg gtaataagcc agtatgcggt cgcggtagtt accttctccg
    29101 gattgcagca actccgtcag tcgctggtga tatgcggcgt aatggcgctc aagcatggca
    29161 acgccaaacg cttctttaga gcgaaagtag tgatagaagg accctttcgg cacttcagcg
    29221 gtttttagta attcgcttag ccccatcccg gtgaatccac gttgcaggca aagttgctcg
    29281 cccgtcgcca ggagatgttc acgagtatca tgttcggtgt gtttgttcat gtgttggagt
    29341 gtagtagacc agtcggtcta gtgcaagcaa agttgctact ttggacatta acagcttcaa
    29401 taatggtaat aatcttatct gaggagggcg aagtggcgga gcaattagag ttctttcccg
    29461 tccagagccc gtgccgggga atttgccagt ctgacgaacg cgggttttgt cgcggctgtt
    29521 ttcgaagccg ggatgagcgt tttaactgga ataaaatgag cgatggcgaa aaacaagagg
    29581 tgctgcgcct ttgtcggcaa cgattaatgc gcaaattacg tgcaaataag ccagcgtcat
    29641 cagacgaacc ggagcaacca tcactctttt gagcctaatc ttgcgtatac tcctctcata
    29701 tctgataaga ggaagcgatt atggttcagc gtattactat tgcgccgcaa ggcccggagt
    29761 tttcccgttt tgtgatgggc tactggcgat tgatggactg gaatatgtcc gcccgccagc
    29821 tggtcagttt tattgaagag catctggatc tcggcgtgac caccgtggac catgctgata
    29881 tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc gctgaaactg gcacctcacc
    29941 tgcgtgagcg gatggaaatc gtcagtaaat gcggtatcgc gacgaccgcg cgtgaagaaa
    30001 acgtcattgg tcattacatc actgaccgcg atcacatcat taagagcgcc gaacagtcgc
    30061 taattaatct tgcgaccgat catctggatc tgctgttaat ccaccgcccg gacccgttaa
    30121 tggatgccga tgaagtggcg gacgcgttca aacatctgca tcagagcggc aaagtgcgtc
    30181 attttggcgt atcgaacttt acgcctgcgc aatttgccct gttgcaatca cgtctgccgt
    30241 ttacccttgc cactaatcag gtggaaatat ccccggtaca tcagccgtta ctgctggatg
    30301 gcacgctcga ccaactgcaa caactgcgtg ttcgtccgat ggcgtggtcc tgccttggtg
    30361 gtggtcgtct gtttaatgat gattatttcc agccgctgcg tgatgaactg gctgtggtgg
    30421 cggaggagtt aaacgcgggc tcgattgaac aggtggttta cgcctgggta ttacgtttac
    30481 cgtcgcagcc gctgccaatt atcggctcag gtaaaattga gcgcgtacgg gcagctgtcg
    30541 aagcagaaac actgaaaatg acccgtcaac aatggtttcg tatccgtaaa gcggcactgg
    30601 ggtacgacgt accgtaagca cttttaggaa tagccgccgt tcaaaaatgt gtcactggtt
    30661 tacacttatt caggaatgca caatgaacgg aggtcctatg aaacgtttta gtctggctat
    30721 tctggcgctg gttgttgcaa ccggcgcaca agctgccagt gaaaaagtcg agatgaacct
    30781 cgtcacgtcg caaggggttg ggcagtcaat tggtagcgtc accattactg aaaccgataa
    30841 aggtctggag ttttcgcccg atctgaaagc attacccccc ggtgaacatg gcttccatat
    30901 tcatgccaaa ggaagctgcc agccagccac caaagatggc aaagccagcg ccgcggaatc
    30961 cgcaggcggg catcttgatc cacaaaatac cggcaaacat gaagggccag aaggtgccgg
    31021 gcatttaggc gatctgcctg cactggtagt caataatgac ggcaaagcta ccgatgccgt
    31081 catcgcgcct cgtctgaaat cactggatga aatcaaagac aaagcgctga tggtccacgt
    31141 tggcggcgat aatatgtccg atcaacctaa accgctgggc ggtggcggtg aacgctatgc
    31201 ctgtggtgta attaagtaat taagaggcca gcgtaccttg cggtggtgct tgctcaagtt
    31261 gcgaaagcga gcagtacagc cgccagacaa ttgccgccag ctcacgggcg gcaggttgat
    31321 gatgacgggc aaggctgtcg caaatccgct gtaattcttc aagtgtggcc gccagtgatt
    31381 tttgctgaac gccacgctca ctcatcactc cccgcaacag tgaaatacag ttatcccgta
    31441 ctcgcgataa cggatcggaa cgcgattccc agtcgcgtaa ttgccagaca acatgagaac
    31501 agttcagcag cactacaccc cagcgtaata accaacggcg agccagcgca tcctggctgt
    31561 tactcaactg actgacgtga tgataagtga gcgattcaaa ttcgctttca ctcagtgttg
    31621 gatggcggct tagttgatcg acaaaatccc gacgcagcgc gcgaatatgg cgacggcttt
    31681 tacgagcatc cgatcctgga cgcagaatgg cgaacgctaa ccacgccaac gcgacgccaa
    31741 cgattttcgc cagattatcg ttaagaaaat cagcaaaatc atacaccggg ggattagtga
    31801 cagcgataaa cgaacccata aaaacaatca gttgccccca cagtgcggca aattttggca
    31861 tctgcaattt aagaagctgc atcgtcgcca gcagtggaaa gagaaacagc aaaaattgcc
    31921 acagatcact aatctggacc atcaggccaa atttgaccac aaagctgaat agcgaaagta
    31981 acaccagcgt gcgcatcagc agtgacaacg acttaaacgg tgctgcgacg gcggagtaga
    32041 gtacgcagct aattgctgcc agcgttaatg cattggcacc ggcatcccat tgcgaagcaa
    32101 tactccatgc gccaatcatc atcaacgtac aaaatgtacg cagcccgctc cacatagctt
    32161 cggcgttatc ggtatgacga gccaggccgg atgtccgtgg gagtctggta tgatcatcta
    32221 caccgctttg cagacgatgt aattcctgac tactttgcag ataaaggcgg caaaaataac
    32281 gtagtcgctg ccagaaggcg acgtgccgat agtcggcgac gttggtcggg cgtagcgggg
    32341 cgataatacg tgcgacggtg taaacatctg tttgcgaact ggcgagcgcc gtcagcaact
    32401 gttcgagaat ttctcgtgtg gcacctggcg gtgagggcca gttgagcaac atacgtcgca
    32461 ggctggagat gacactggtc atacgtaatt gctggtggag cagcgcatta aggcgcgcgt
    32521 tttgctggcg aaaacgatag tggctccaga aagcctggat acgcagcaaa ttcatggtca
    32581 gtatctgccc aatcacccct tcatgtgctg cacgaatggc atcggttgtt tcaggctgcc
    32641 agagtaaact ggcatgttcc agtaatcggg cgtgcatgtt tttcaatgcg gttaaaaggg
    32701 cagtagcatc ggaactgctc ggcaggatca tcatcatcat gccgccgcac aaaataccga
    32761 caatcacctc gcaaacgcgc gcctgagcga tatcccacag ctggctggcc tcagtaatat
    32821 taaccatcgg aaaggcgatg atggcagccg tgtagcccgc cagttgaaat gcatacgcga
    32881 cgttattcgt gaagtgcgca caggcccagg tacaaaagcc aagccacgcc gacatgctca
    32941 atagaaaaaa ccacggctca ttgagcgtat gtccggcaag aagcagtgcc gcaatggctc
    33001 ccagtaaact gccagcgatg cgtccgaggc ttttgctgat gacaccgcca acggtgggaa
    33061 agctaaccac tgcagccgag gtcatcgccc aatagggttc atccagattc aaataatagg
    33121 caaccgtcag cgccagacac atagcaatgg tattgcgtaa cgcataacgc cattgcgcca
    33181 gcgtggccct gaaccagggc aaattgcgca aggaccatga cgatgcgttc attattgacc
    33241 tacggcaatg ctacaggtgg tgcccgacac cagcgtgaca tcgcctggaa ctttatccag
    33301 cgcaaagcgg acgggaacgc gttgggcgag acgaacccag gggacgttag gtttcacatc
    33361 cggaataagg cttgagtcac tttcaacgct ctgatcataa atcgcccgac cgatactgaa
    33421 aacgtgaccc tgtaacgttt tgttatcact atagagggta atttgtgctg gtgcaccttc
    33481 gcggatatgg cgcaacttgg tttcttcaaa ataaccaatg acgtaaaacg agtggctgtc
    33541 gacaagggca aacagcggtt tcccggtgtc agcgtaatcg ccgatgcggg tagtgaggtt
    33601 agtcacccat ccactcaccg gggcgcgaat ttccgtttgc gccagttgcc attgtgtctg
    33661 tttcagcgtc gcctgtgcgg catcaacact ggcctgcatc gctttaacat tgaggttagc
    33721 ggtatcgagt tcttcggcag aaataaaatt ttgtgagaga tgacgacgac gattcgcttc
    33781 gttattggct tttgcgaggt cagattgcgc tttcgccagc tgtgcctgtg cgttcagctc
    33841 ggcgatctga aagggcgttt tatcgatcgt gagcagaaga tcccccgcgt taaccagctg
    33901 gttgtctttt atattcagct caacaatacg tcccgacacc tgtggggtga tagaaacctg
    33961 ttctgcgcgt attttcccgt cgcgtgtcca cggagattgc atgtaataat tccacagcca
    34021 ccatccggca agaaccgcaa ctaccgctac aatgattgtt gagaaatact taattgtttt
    34081 aattgacata gttaccacgc aatcagtatt gccagagcaa ggcaaacgca aatcgcaaac
    34141 agcgataaat ccattaacaa gggatgccag atgtcaccgg cgtagatcca gccacgaagc
    34201 aggcggtgta cgacaagcca gatgacgaat ccaaacgcga atgctttgaa aaacggagga
    34261 aagtagacgg acgcaccgaa caccagatct tgtaagggca atcctgttgc attgagcata
    34321 aacttcacaa agtagattcc tttacgaccg aatatattgc gttagattaa taaatattct
    34381 ttaagtgcga aaaatttacg cgcaatttct gaaaaatatg tctgacggta accaaatgca
    34441 gcaatacatt tgttttagca atacaattgc tgcacactat tctaaaagcc gcataatatc
    34501 ttagcaagct aattataagg agatgaaatt ggaatcgcca ctaggttctg atctggcacg
    34561 gttggtgcgc atatggcgtg ctctgataga ccatcgcctg aaaccgctgg agttaacaca
    34621 aacccattgg gttacgttac acaatatcca tcagttacct ccagaccagt cgcaaattca
    34681 actggcaaaa gcgattggca tcgagcagcc atcactggtc cgtactctgg accaactgga
    34741 agaaaaaggg ttaatttcgc gtcaaacttg tgccagcgat cgtcgggcta aacgtattaa
    34801 actgacggaa aaggcagagc cgctgatcag cgaaatggaa gctgttatta acaaaacccg
    34861 cgcggaaatc ttacatggca tctccgcaga ggaactggaa caactgatta cgctcatcgc
    34921 aaaacttgag cataatatca ttgagttaca ggccaaaggg tgaaatgaag ggggcgtgtg
    34981 gtcagtaacc tgaccacacg caaacttatt tagcgcggag aaacggtcac ctgactgcca
    35041 ttgctggcca gtacgacacg ttggcccgga gagaaacgag tgttgccttg tttctgtacc
    35101 accatgatgg tattaccatc gtctttacga atttccagct cgacaccttg cgttttgttc
    35161 attgcactct gtacgccctg accagctacg ccacctgcaa cagcgcctgc tgcagtagcc
    35221 agagaacgcc cggttccgcc accaacagta tttcccagga aaccaccaag aacagcaccg
    35281 ccaattgcac cgataacgtt ggaatcatca ccgccctgaa tctgtaccgg acgtacgtta
    35341 acgatggttc catagctgac attctgtact tgttttgctt cagaagcggt ataaacatcc
    35401 cctgacaggg tgtcgttatt aacacaaccg acaagagaca gacctaccat tgaaacaacc
    35461 aatacgcgtt taatcattga aaaatctcct gttcaccatg aaacgccacg caagcatccc
    35521 tcatggtcaa agtatatggc atatgtgaat ccatggttac atgttctacc aaatatgagc
    35581 aaatcataat gaaagtagga tcaaccaatt ataaacaaaa cattcatgat gattattgat
    35641 ttgaagataa tgttttacta ccaacggtaa aaatcggtca ttccgcatgg tattcacgaa
    35701 aagtttatgt ttgagtggtg cgcaaacgtc agattgaaaa aggatgaact atgaaatcgg
    35761 gccgctttat tggcgttatg tcaggcacca gccttgatgg tgttgatgtt gtgttggcga
    35821 caattgatga acaccgggtc gcacagctgg caagtttgag ctggccgatc ccggtgtctc
    35881 tgaaacaggc tgtactggat atttgccagg gccagcagct tacactttcg cagtttggac
    35941 agcttgatac tcaactcggg cgactttttg ctgatgcggt caatgccttg cttaaggaac
    36001 aaaacctgca ggcgagagat atagttgcga tcggctgtca cggtcaaacc gtctggcatg
    36061 aaccgacggg cgtggcacca cacactttac agattggcga taacaatcaa attgtggcac
    36121 gcaccggaat tacggttgtc ggtgattttc gccgtcgcga tattgccttg ggaggacaag
    36181 gcgcaccgct ggtacctgcg ttccatcatg ccttgctggc tcacccaacc gagcgacgaa
    36241 tggtgctcaa tattggcggc atcgccaatc tgtcactgct cattcctggg cagccggttg
    36301 gcggctacga taccggtcct ggtaacatgc tgatggatgc ctggatctgg cgtcaggccg
    36361 gtaaacctta cgataaagat gccgagtggg cacgggcggg taaagttatt ctcccactgc
    36421 tgcaaaatat gctcagcgac ccatatttct cgcaacctgc accgaaaagc accggacgcg
    36481 aatactttaa ctacggctgg ctggagcgcc atttacgcca ttttccgggt gttgatcccc
    36541 gtgatgttca ggccacactg gcagaactca ccgccgtgac catttctgaa caagttttgt
    36601 tgagcggtgg ctgcgaacga ttgatggtat gtggtggagg tagtcgtaat ccgctactca
    36661 tggcgcgtct ggcggcatta ctgccaggca cagaagtcac caccaccgat gccgttggca
    36721 ttagtggcga tgacatggaa gcattggctt tcgcctggct tgcctggcgg acgctggcag
    36781 gattaccagg aaatctgcct tccgtcactg gcgcaagcca ggagacggta ctgggggcta
    36841 ttttcccggc taacccgtga tatcagagtt aactgaattt ttcttaccgc gtctgccgtt
    36901 acacttggaa cgttagggag ggcgtattgc cctccagacc aggaaagtct tcgggaaatg
    36961 tctatgaaaa aactgttact gatctgtttg ccggtgcttc tcaccggctg tagcgcgttc
    37021 aatcaactgg ttgagcgcat gcaaaccgat acgctggaat accagtgtga tgaaaaaccg
    37081 ttgacggtca aactgaataa tccgcgccag gaagtcagtt ttgtttacga taatcaacta
    37141 ctgcatctca aacagggcat ttcagcctct ggtgcacgtt acactgacgg aatctatgtt
    37201 ttctggtcga aaggcgagga agcgactgtc tataaacgcg accgcatcgt cttgaataac
    37261 tgtcagttac aaaattcacg gcgttgagat ttttccaggg gcggcgcaca atagcgtcac
    37321 ccactgacaa tccgtaaaga aaaccatgtc tgataacgac gaattgcagc aaatcgcgca
    37381 tctgcgccgt gaatacacca aaggcgggtt acgccgccgc gatcttcccg ccgatccatt
    37441 aacccttttt gaacgttggc tctctcaggc ttgtgaagcc aaactggcgg accctaccgc
    37501 gatggtggtc gctaccgtgg atgaacatgg tcagccttat cagcgcatcg ttttactcaa
    37561 acattacgac gaaaaaggca tggtgtttta caccaacctc ggtagccgta aagcacatca
    37621 aattgaaaat aatccgcgcg ttagcctgct gttcccgtgg catacccttg aacgccaggt
    37681 gatggtgatc ggtaaagcag aacgactttc gactctcgaa gtgatgaaat attttcatag
    37741 ccgcccgcgt gatagccaga ttggtgcatg ggtttcgaag cagtccagtc gcatttctgc
    37801 ccgcggtatc cttgaaagta aattcctgga gctgaagcag aagtttcaac agggcgaagt
    37861 gccattgccg agcttttggg gcggttttcg cgtcagcctt gaacagattg agttctggca
    37921 gggcggtgag catcgcctgc atgaccgctt tttgtaccag cgtgaaaatg atgcgtggaa
    37981 gattgatcgt cttgcaccct gaaaagatgc aaaaatcttg ctttaatcgc tggtactcct
    38041 gattctggca ctttattcta tgtctctttc gcatctggcg aaaagtcgtg taccggcaaa
    38101 ggtgcagtcg ttatatacat ggagattttg atggcaagca gtaacttgat taaacaattg
    38161 caagagcggg ggctggtagc ccaggtgacg gacgaggaag cgttagcaga gcgactggcg
    38221 caaggcccga tcgcgctcta ttgcggcttc gatcctaccg ctgacagctt gcatttgggg
    38281 catcttgttc cattgttatg cctgaaacgc ttccagcagg cgggccacaa gccggttgcg
    38341 ctggtaggcg gcgcgacggg tctgattggc gacccgagct tcaaagctgc cgagcgtaag
    38401 ctgaacaccg aagaaactgt tcaggagtgg gtggacaaaa tccgcaagca ggttgccccg
    38461 ttcctcgatt tcgactgtgg agaaaactct gctatcgcgg cgaacaacta tgactggttc
    38521 ggcaatatga atgtgctgac cttcctgcgc gatattggca aacacttctc cgttaaccag
    38581 atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg aagatcaggg gatttcgttc
    38641 actgagtttt cctacaacct gttgcagggt tatgacttcg cctgtctgaa caaacagtac
    38701 ggtgtggtgc tgcaaattgg tggttctgac cagtggggta acatcacttc tggtatcgac
    38761 ctgacccgtc gtctgcatca gaatcaggtg tttggcctga ccgttccgct gatcaccaaa
    38821 gcagatggca ccaaatttgg taaaactgaa ggcggcgcag tctggttgga tccgaagaaa
    38881 accagcccgt acaaattcta ccagttctgg atcaacactg cggatgccga cgtttaccgc
    38941 ttcctgaagt tcttcacctt tatgagcatt gaagagatca acgccctgga agaagaagat
    39001 aaaaacagcg gtaaagcacc gcgcgcccag tatgtactgg cggagcaggt gactcgtctg
    39061 gttcacggtg aagaaggttt acaggcggca aaacgtatta ccgaatgcct gttcagcggt
    39121 tctttgagtg cgctgagtga agcggacttc gaacagctgg cgcaggacgg cgtaccgatg
    39181 gttgagatgg aaaagggcgc agacctgatg caggcgctgg tcgattctga actgcaacct
    39241 tcccgtggtc aggcgcgtaa aactatcgcc tccaatgcca tcaccattaa cggtgaaaaa
    39301 cagtccgatc ctgaatactt ctttaaagaa gaagatcgtc tgtttggtcg ttttacctta
    39361 ctgcgtcgcg gcaaaaagaa ttactgtctg atttgctgga aataatgcat taagtggaaa
    39421 gggggagtga gaaatcactc cccctggttt ttatacaggg aacatgatga aaaatattct
    39481 cgctatccag tctcacgttg tttatggtca tgcgggtaac agtgcggcag agtttccgat
    39541 gcgccgcctg ggcgcgaacg tctggccgct gaacaccgtt caattttcta atcacaccca
    39601 atacggcaaa tggactggct gcgtgatgcc gcccagccat ttaaccgaaa ttgtgcaagg
    39661 cattgccgcc attgataaat tacacacctg tgatgccgta ttaagtggct atctgggatc
    39721 ggcggagcag ggtgaacata tcctcggtat cgtccgtcag gtgaaagccg cgaatccgca
    39781 ggcgaaatat ttttgcgatc cggtaatggg tcatccggaa aaaggctgta tcgttgcacc
    39841 gggtgttgca gagtttcatg tgcggcacgg tttgcctgcc agcgatatca ttgcgccgaa
    39901 tctggttgag ctggaaatac tctgtgagca tgcggtaaat aacgtcgaag aagcggttct
    39961 ggcagcgcgc gaactcattg cgcaagggcc acaaattgtg ttggttaaac acctggcgcg
    40021 agctggctac agccgtgacc gttttgaaat gctgctggtc accgccgatg aagcctggca
    40081 tatcagccgt ccactggtgg attttggtat gcgccagccg gtaggtgttg gtgatgtgac
    40141 gagcggttta ctgctggtga aactgcttca gggggcaacg ctgcaggagg ccctggaaca
    40201 tgtgaccgct gcagtatacg aaatcatggt gaccaccaaa gcaatgcagg aatatgagtt
    40261 gcaagtggtt gctgctcagg atcgtattgc caaaccagaa cattacttca gcgcaacaaa
    40321 gctctgaaat atctcatttc ggcccacaac gacctgtggg ccgacggcta ttactttaag
    40381 ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag ccatacgttg cataaatgcc
    40441 gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt atgcccagcg cagaaccgta
    40501 aacagatagg catcagcaat tgtaaatctt tgcccgcaga tccagtgctc atccttcagt
    40561 gcctcgttca catattgcag cttcttctcc agttgcgcgc gaactgtcgg tttgtactct
    40621 tccggtgtat cagggcgaaa caaaggtgtg aaacctttat gcagctcggt ggcgatgtaa
    40681 ttcagccatt cgatggtttt atagcgggaa atactgttta ccggtgccag caactggcgg
    40741 tcggggacgc tgtcggcaag atactgcata atcgctacac cttccgtcag caaagtaccg
    40801 tcatccagca gcaatgcagg tacctgtccc ttagggttaa cggcaaagta atcgtcacca
    40861 ttttcgagac gttttttcat taaatccaca ctgacgagcg taaaatcctt tccgctctca
    40921 cgcggggtga tatgggaagc gagagagcag gcgcccggtt tgtagaacaa tttcatcggt
    40981 aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaaaagccg
    41041 ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta cggctgcttt
    41101 cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttcggcg cggtcagcag
    41161 catcagtact gcaataacgg cagtagcgac accaatctgc aagaatacgc gaccatagac
    41221 ttccagtgac atcagcggat cggtaacgtt atccggcaca gccatcatac ccgcaacata
    41281 accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa tgaagcccat
    41341 cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga tcatcagttc
    41401 cccgatactc tgcaggccat agctcgcgac cagccagctt acagacacga tacctgcgtc
    41461 agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca tcaccatacc
    41521 gattgcaaac ttggttggca tcggcagggt atcgcccatc ttgttataga tagcggccag
    41581 aatcgggcta ccaatgatga tccagaacgg gttcagtgcc tgatactgct caggttctac
    41641 ggccaggccg agaatggtgt gttcaacgtt acgaatcgca aagaagttca gtgacgttgg
    41701 catctggctg tacagcacga agaagataat ggcttcgagc atcaggatga aggcaacgat
    41761 cattttacga cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga taaccacgat
    41821 accaaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca gcagccaggt
    41881 ggcgatagcg atcagtgcca caacaccaat aatggtcagc agcaggttac ggtagttgat
    41941 aggctcgaag tctggttttg aaccgtactg tttaacccag cgttggcaga aggcgaagtt
    42001 aacgatagtg atcagcaggc ctacaacgct caatgcaaac gcaacgctcc agccgtattt
    42061 cgcggccagc cacggggtgg cgatcataga gaagaaagag ccgatgttga cggacatgta
    42121 gtacatggtg aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg tagaaagcag
    42181 agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca tacccatata
    42241 aacgataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg ccagcacaat
    42301 agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc agccgccgat
    42361 agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg aatccgcttc
    42421 agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc cgtaataacc
    42481 aaaacgttcc cataactcaa tcgagaaaat gagatagaac gccttcggtt gtttgaaagc
    42541 gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta cctctttttt
    42601 tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt
    42661 gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc agacaagtcc
    42721 tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg ggaaatgtta
    42781 tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat tgagaggggg
    42841 atgatggctt gacggcgata tgttctgcta tttagcgttt ttcctagtga gatagtccag
    42901 tttctgaaaa atagccagtg taatgttttg taggtcaata tttgttgcgt gtgtaaattt
    42961 aaatgatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg tgatcttgat
    43021 cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt ttggttatcc
    43081 gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta aaaagatgtt
    43141 ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct tttcttcaga
    43201 tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca cagcggggct
    43261 tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag tcgactttaa
    43321 actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt ttccccggcg
    43381 caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc
    43441 cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc agggcttcaa
    43501 gcgcagcacg atcttccgga acctcgccat tatgctgctc cagtaagata cggcaggttt
    43561 tgatgatatt ttctgctttg ctgttataaa gcccaatcgt tttgatatag gttttcaccc
    43621 cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc caccgggtag agtttcgccg
    43681 tcgccttatt aacactgaca tcggtcgcct gagcggaaag cagtacggca atcagcaatt
    43741 caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca cgcaggcgag
    43801 tgaggatctc caggcgtttt gctttgttca tcagacattc cctgtttcac cgtttggcaa
    43861 tgcacgttcg gcagctgctt cagtacggcg ctttttcatt ctttcatcaa tcaggtattt
    43921 tcctgccagc atcagtccca ggccaataaa tgcacctggt ggcagcatcg ccagcaggaa
    43981 aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac ctaacagcgc
    44041 atctgcaccg tcaaacaatg tgccattgcc gataatttcg cgtagtgaac ccagcacgaa
    44101 catggcgcag gttgcgccca taccaattga aaagccgtcc agtgccgaaa gcgccggacc
    44161 ttttttggcg gcgaaggctt cagcacggcc cacaacgata cagttagtga caatcagcgg
    44221 aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca gcatctgtac
    44281 agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct cggctggcgt
    44341 ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag
    44401 tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc aacagaggac aaaggccgag
    44461 caactggacc agcgcagagt tgtttttcca caacccctga acaataacgt ctttaatttc
    44521 gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta acgtctgagc
    44581 gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt
    44641 gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat ccgcaccact
    44701 gattttttta cccgcaaaat gggtgatcca gtcagaaagg cgcagttcga ttttatcgcc
    44761 aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc cgttaaaatc
    44821 ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg cttccagaac
    44881 ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac ctttacctaa
    44941 ctctggcgca gttaccagat agcaactctg tgccagcgca ttgttatagc gttcggctgg
    45001 cagcacctga tcaaataatg ccttttgttg cagactggcc tgttcagcaa tcgtcgtttt
    45061 ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc gctgcaaata gcgctaacgt
    45121 aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg
    45181 cggacgggtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga cggcaaaagc
    45241 cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc ccgcaagcgc
    45301 gccgaaaata agacgaccac gattggtcgt agaagcggta accgggtcag tcaaaataaa
    45361 gaatgcgccg agcatggtcg ctccagacag cagatgaatt tgcggtgctg ccagtgtttc
    45421 tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga agctgagggg
    45481 aacatgccag cgaatcgctt tctgccatag caaccatacg ccgccagcca gccaggcgag
    45541 atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat attgcataat
    45601 ctgttcaacc gaatgaccgg cacggacaga ggttttaaat gtatccagcg gtgtcgcctg
    45661 actaatgcca tcaataccta agcgtagcgt gttcatatca ccaccactgg cggtatgacc
    45721 gctgaaaata acctggatgg cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg
    45781 cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat aaccaatcat
    45841 tgccggatta aacgggtttt gtcccagacc gccatataac tgtttagcga tgatcaccgc
    45901 aaacaccgta cccagcacga ccatccacca tggcgcgagg gggggaatac ttaccgccag
    45961 caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg actgcttgcg
    46021 tagtttgagt acgagagctt cggctaacag agcactaacc gatgccaaca ggatctgaac
    46081 gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg ctgcgagcaa
    46141 cactaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg agctagctat
    46201 tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt tggcctgaac
    46261 gcgggcaata gccgcggcaa ctgccgcttt gtgcggatca acctgttctt ctggttctgc
    46321 gttagcctgt tgctgttcca gcttgcgcgc tttggcgcgt gcaatagccg cttcgacggc
    46381 agctttgcgc ggatcaacct gttcttctgg ctccgcatta gcctgttgct gttccagctt
    46441 gcgtgctttg gcgcgtgcaa tagcggcttc gacggcggct ttgcgcggat cgaccggttc
    46501 ttctggttcc gcattagcct gttgctgttc cagcttgcgt gctttggcac gggcaatagc
    46561 ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat tggcctgttg
    46621 ctgttccagc ttgcgcgctt tggcgcgggc gatagcggct tcgacggcgg ctttgcgcgg
    46681 atcgacctgt tgttctggtt ccgcattagc ctgttgctgt tccagcttgc gtgctttggc
    46741 acgggcaata gcggcttcga cggcagcttt gcgcggatcg acctgttgtt ctggttccgc
    46801 attagcctgt tgctgttcca gcttgcgcgc tttggcgcgg gcgatagctg cttcaacggc
    46861 agttttacgt ggatcagtaa cggttgctgc gtcgttagtt tgctgcagtt ctgcctgttt
    46921 cgctctggct tgcgctttac gggcttcccg tgctgcaata attgcactgt tatccgggcg
    46981 ttcgcccgct ttaatcacaa taggctgtgt agcctgggcc tgtttctctt tcacccgcgc
    47041 cagagcggca gcaatcgcat ctttatcttt ggctgcaggt tgaacggctg cgctcttatg
    47101 tcgttcaagg cgagccgctt tttcgcgctc cagacgagcc tggcgcgctt cgaaacgcgc
    47161 tttggcttct gcggcgcgct tttcttcctg acgaatagcc gcaatttcag ctttttcctg
    47221 acggaaatat tgcaccaggg gaatattgct cgggcaaacc cacgcgcaag ccccacattc
    47281 aatgcagtcg gcaatgttat gcgtggtagc tttatcgtgt tgctgacctt tgctgaacca
    47341 gtacaactgt tgcggcaaaa gatccgcagg gcaggcgtca gcacaggcgc tacaccggat
    47401 gcagctttgt tcttcctgtg gttcgccaag ctcattggca gagggagcca acagacagtt
    47461 ggtaatcttt acgaccggga catccagcca tggcaaggta aagcccatta gcgggccgcc
    47521 cataatcacc atttgatcgg cagaggggca gaatccggcg tcattcaata aatgacgcac
    47581 tggcgtcccc agccgtgccc agacgttgcc cgggcgagcg attgcttcgc cagtcagggt
    47641 tacgacacgc tcggtaatcg gctcgccatc aataacggca cgtttcactg cataagcagt
    47701 gccgacgttt tgcattaata cgccgatatc ggatgaacgc ccgccatgtg gaacctgctt
    47761 cccggtcaga atgtaggtta attgtttagc accgccagaa ggatatttgg ttggaatcac
    47821 ccgcagagaa atatcgtgag agtccgccag caccgcgcgc agcatggaaa tcgcctgcgg
    47881 tttgttatct tcaatgccga taagaatttc gcgtggctgc agaatatgcg caagaatgcg
    47941 aataccctct acgacctgag ccgcgcaatc ctgcattaaa cggtcatcgg cggtaatgta
    48001 cggctcgcac tcagcagcgt tgataatcaa cgtctcaatc ttatctccgc cgccctgcaa
    48061 tttaacgcct gtcgggaatc ctgcaccacc cagccccgca acaccaaact gatgaatgcg
    48121 ctcgattaac tcttcgcgac tgcgagagcg ataatcggcc cagccgtctc gcgggatcca
    48181 gcagtcttca ccatcggcat caataatcac gcttaattca gctaaagctg aaggatgagc
    48241 cgtagagtgg ggcgcaatag ccgtaacggt acccgaggtg ggcgcgtgaa caggcagcat
    48301 tttgccgcga ccacgggtaa gcggctggcc gcgcaatact ttatcgccga cgctaacgca
    48361 caactcacct tcagcgccaa tatgctgttt cagtggaata acaaaacgct gcgccagggg
    48421 tacctggcgc aggggtgtac cgttggactg ggttttcatc tccggtggat ggatgccgcc
    48481 gttgaaatcc cagattttat tttttctgaa tgtagagaat aacttaagca tggtgttcca
    48541 cgggaatgat acgcacggga atggtgttca gatcccattt ccaggagtca ggtgtttctg
    48601 cgaccggttg caacgagatg cagtgcgtcg ggcacggatc aacacataaa ttgcagcccg
    48661 tacagagatc actcattacc gtatgcatgg cacgggtaga gccaacgatg gcgtctaccg
    48721 gacacgcctg aatacattta gtgcagccaa tacagttatt ttcatcaata accgccacca
    48781 tccgcgcagg cgttagctct tgcgcttcgc catccagcgg ctgcggctcg acattaagca
    48841 actcggcaat ttttagcatc acagcttcgc caccaggggc gcaacggttg attttttcac
    48901 cgttacagct gatggcttcc gcgtaggggc gacagccggg ataaccgcac tgaccacact
    48961 ggctctgcgg taagatttcg tcaattttct caacgaccgg atcgtcttcc accgcaaaac
    49021 ggcgggaggc ataacccaga atggcgccaa acgccaggcc cagcaggctc acggcggcaa
    49081 cggcaatcca gatagcattc attacaactt caccaaacca ctaaagccca taaaggccag
    49141 agacataaga cctgcggtaa ttaacgcaat ggcattaccg cgaaaaggtg ccgggacatc
    49201 agccacggca aggcgttcgc ggatggctgc gaagagaacc atcaccagcg aaaaaccgac
    49261 agcggcggaa aaaccgtaca gcgccgactg caagaaattg tgcccgagat tgatattcag
    49321 caacgccacg ccgagcactg cacagttggt ggtgataagc ggcaaaaaaa tccccagcag
    49381 acggtaaagc accgggctgg ttttgcgcac caccatctcg gtgaactgca caaccacagc
    49441 aatcaccaga ataaacgcca gggtacgcag gtaaatcaga ttaagtggga tcaaaatcca
    49501 cgtatcgata agccaggcgc aaatagacgc cagcgtcatc acaaacgttg ttgccagccc
    49561 catgcccatc gcggtttcca gctttttgga aactcccata aacggacaga gaccgagaaa
    49621 cttgaccagt acaaagttat tgaccagtac agttccaaca aacagtaaca ggtagtcagt
    49681 catttttcgg cctgaaataa aaaagccgcc tattatccgt taatccagag caggcgacaa
    49741 caggttaact gtaaggttat tacggattca caaacgtcgc tttcacccgc gacgagcgtt
    49801 tgaaataggg gacgatcaat gcggttgcca acaaagtaaa cagcaactgg cgaacggcta
    49861 tgccatcttc cacgggtgaa aaagcgaacg cttttactgc cagcaatacg gaaataagca
    49921 gccagataat atagtgttta ggaacacaac gccgacgttt aaagaaagcg atggtcaacc
    49981 acagtgtgta ataccacatg gcgatagcag taataaaaga aacgccccat aagatttgtg
    50041 tcgtcagtgc ttgtgcgccg agcgtttgaa atgtttgcgg agaagataac gcagcggtat
    50101 acagcaacaa cgccagcgtc gtacttaaca gagcgactaa aagccaggcc aacgggccaa
    50161 gcaaccagcc tccaatacgt tgtggcgttg tggtggtcat aggttctcca gaatagcgac
    50221 gcaaacaggc aaatctaatt ttaggggcgt tattataagg cttttataac cgatcgctac
    50281 tttctttatt ggacatagtg ccagacggac ttcggtacct ggccgaggtc aaacaaacgc
    50341 ccgccagaaa ccagctctgc gcgacggtga tcggcagcac gatacatatt gatcagttcc
    50401 tgatcgtccg tcagggtgta attaagatgg tcgtaaagtt tttccagact ttcgagtgaa
    50461 ctgattttgc gaaattttaa taagtagtcc tgaacagtca taaaaggtcc ataaaataat
    50521 taataccaaa ggggtaggta ttattagtga tgataaaatt tcaccaaaag tgaacgcttg
    50581 tgttattaca ataataagaa tgcgctgatg gcaaatattc agggatgaaa aactgctatg
    50641 aaaagagtaa cgcgctttac tatccctgac aatcaccaac aacaccgaac aagataataa
    50701 attcctggtt taatatccga caagtgaaaa catgcaccct gacgggcagc atgtcgctct
    50761 acaagcgcag agcttacttg tgttgtaccg tagtactctg ttcaggtggc tgatagttgt
    50821 caatgtgact cgccacgcca agaagaatga ctgaaacgac aagaacgatc caacctgtta
    50881 attcaataag acgattcatt acaactcaca tctctgttga ttgatccatt aacttcaggg
    50941 ggtaaatgtt acttagcaat aatagctcca gcaagatttt tactgaggtt tttgcgatat
    51001 ttagcttttg tcgttggaaa aatcgctatt tttttgactt aagttaaaca acatccctta
    51061 ttgctggcag gttattaaac tgttgagcga gggtaaagga tagtgtcaaa tagccatcat
    51121 acttcaatga gaggcaatga catgagcgac aacatccgtg ttgggttgat tgggtatggt
    51181 tatgcgagca aaaccttcca tgcgcccctg attgcgggca cgcccgggct ggaactggcg
    51241 gtaatctcca gcagtgatga aacaaaagta aaagccgact ggccaacggt tacggttgtc
    51301 tctgagccga agcatctgtt taacgatccc aacatagacc tgattgtcat tcctacaccc
    51361 aacgataccc atttcccgtt agccaaagca gcgcttgagg cgggtaaaca tgtggtcgtt
    51421 gataaaccct ttaccgtgac actgtcacaa gcgcgagagc tggatgcgct ggcaaaaagc
    51481 ctggggcgtg tgctgtctgt attccataac cgtcgctggg atagcgattt cttgacgcta
    51541 aaaggtttac tcgcggaagg cgtgctgggt gaagtcgctt actttgagtc tcattttgac
    51601 cgcttccgtc cgcaggtgcg cgatcgttgg cgtgaacagg gtggcccagg cagtggtatc
    51661 tggtacgatt tagcaccgca tcttcttgat caggctatta cattatttgg tttaccggtc
    51721 agcatgacgg tggacttggc acagttacgg cctggagcgc agtcgaccga ttatttccac
    51781 gccatcttgt cctatccaca gcggcgagtc attttacacg gtaccatgct ggcagccgct
    51841 gagtcagcac ggtatatcgt gcatggatcc cgaggcagtt atgtgaaata tggcctcgat
    51901 ccacaggaag aacgtctgaa aagtggcgag cgtctgccgc aggaagactg gggctacgat
    51961 atgcgtgatg gcgtacttac ccgcgtggaa ggtgaggaac gtgtcgaaga aacgctgttg
    52021 acggtgcctg ggaattatcc ggcttactat gcggctattc gtgatgcgtt aaatggcgat
    52081 ggtgaaaatc cggttccggc aagccaggca atccaggtaa tggagttgat tgagctgggc
    52141 atcgaatccg ccaaacatcg cgcgactttg tgccttgcgt gaaaatggat ggtgcaattt
    52201 gcaccatcca tttttgttac ttcgctgcga ctttttctcg cagtgcgcgt ttttcctcag
    52261 cgctgaggaa agccatttcc agaccattaa tctgtgcctg gcggatttgc tcgcgggata
    52321 acccagcagc tggcgcggca acgttatatt cgtgaatgat atccactccc tgtacgccgg
    52381 gatcgtcagt gttaatgctg gcacgaatgc catgatcaag gaacgttttc agcggatgtg
    52441 cagccagctc agccacggtg ctggtctgaa tattggaggt cagacaggat tcaataccaa
    52501 tttgttgctc ggcgagaaaa tccatcagcg cccgatcttc aatggctttt acgccatgtc
    52561 caatacgctc cgcacccagt tcacgaatcg cctgccagat gctttccggc ccggcagctt
    52621 cgcctgcatg gacggtaata tgccagcccg catcacgcgc gcggttgaag tgagaaagaa
    52681 acagacttcc cgggaaacca agttcatcac cggctaaatc aagtgcggta atctggtcac
    52741 ggtgggctaa aaaggcctcc agctcttgct gacaggcggc ttcgccgaag gttcggctca
    52801 taatgccgat aagcttcgcc tgcacaccaa aggtgcggca accttcacgt acgccatcga
    52861 tcaccgcttc aacaacacct gcaacaggca gctgatgtgc cattgccatg tagcctggag
    52921 aaaaacgcag ctcgacatag tgcaggccgt gacgggctgc atcttcaatg ttttcaaatg
    52981 ccacgcggcg acaagcatca agagaggcga gaactttaac gccccagtca agtttggtca
    53041 gaaagctcac cagatcgggt tcgttggcaa tgacctgaac gtggggaatc agtgtttcca
    53101 gggattgtgc aggaagcgag atattatact ggcggccaag ttcaagaatg gtctggggac
    53161 gaatgttgcc atcaaggtgg cgatgaatat cagttaatgg cagggtggta tcaatcatgg
    53221 tcgcactctt tttctggata taaaagtgcg caccattata aaaacaaagc agggtaaaaa
    53281 tccacgattg cgcaacgatt ttttacgttg cgcaattggg gttagcgaac agcgcggatg
    53341 gcgttaatta atccagccac acctttttcc agtttcgaac gtgggcagcc ggcattgagt
    53401 cggacaaaac cacgaccttc ttcaccgtag gtataccccg gcatgatcgc aactttttct
    53461 tgttcgataa gtgctttttg caacgcgttg tcgtcaatat tcaacggacg taaatcaagc
    53521 caggccagat aagtggattg cgggatctgc cagttgagtt caggaaacgt ggcgttcatt
    53581 ttatctgcga tatacgtcag gttatctttc agatagacgc gtaaggcatc cagccacggt
    53641 gcaccttgct gataggcggc gatatgggca gttaacgcca gtaccgaagg ggaagaaagc
    53701 ccatcaagtc ctttcagtgc cgataaatag gcatcacggc tgctgctatt ttctataatc
    53761 ccgtaagcac cggtcagcgc gggaatattg aaacttttcg atcccgacgt cagcaacgcc
    53821 cagtctcccc gcgccacatt actccaggga atatgcggct gctcgcccca aaccatatcc
    53881 atatggattt catcggaaat aaccctcaca ccatgacgct cgcacaggtc agccatgatc
    53941 tccagctcat cgcacgtcca cacttttccg gtaggattct gtgggctaca caggagcata
    54001 attttacatt ctggtttcgc caacacggct tccagcttgc ccatatcgca aaaccagccg
    54061 tcagcctgct tctctaaagc aacgggcatt actgtgcgct gattaccttc aatggccttg
    54121 taaaatgcgt cataggcggg tgtgtggatc accacgcctt cacctgtttc agaccactga
    54181 cgaatcagtt ctgagaccat atagataaca gaagggccat acaccaccga ctgaggatcg
    54241 atggcggtgt aatgctgggt ggaaaaccag tgggcaatag ccgcgagaaa ctcatcgttc
    54301 ttccagcggc tgtagccaaa cacgccgtgc atcaggcgct gattcagcgc ctcgataatg
    54361 cagggggcag tggcaaaatc catgtctgaa atcgtgaaag gtaacaggtc agcagtgccg
    54421 aaacggtcag cgacataatc ccactgcgta caccatgtgc catgacgatc cacgaccttt
    54481 gaaaaatcga acatatctta tccttatgcc tggacagtat gcatcagacc ggccatttca
    54541 tcttttactg actgaacttg tggcccgata acaacctgca ggttatgttg attaagttgt
    54601 actacgccaa ttgcccgatt gtccttcagt gcctgcacat taacaagcga catatctttc
    54661 acagacaaac gcaaacgggt aatgcagtta tcaaggctga caatattgtc ggcaccgcct
    54721 aaggcttcga ggattgccgg aacgttgtaa cctgatttac ccggcgcacc ggcaacggct
    54781 ttttcgattg aactggccac ttcgctatcg cgccccgggg ttttcagatt gaagcgggtg
    54841 atagcgaaac ggaagatgac gtagtaaacg acaaaccaga ttgccgccac cactggcacc
    54901 atgtaccact tggttgacag accatgcaaa ataccgaaca ccacgaagtc gatgatattg
    54961 ccgtcggtat taccgatggt gacgccgagc acagacatga cggtgaagcc gaggccggtt
    55021 aacagcgcgt ggatgacata cagaactggc gctacgaaca ggaacaggaa ttccagcggt
    55081 tcggtcgtgc cgccaacgac acaggcgatc aggccagaaa tcagcagccc tttaatttta
    55141 tggcgatttt ccgggcgcgc gcagtgatac atagctaacg ctgcacctgg cagaccgccg
    55201 agaaacgcag gcattttgcc ttgcgaaagg aaacgcgtgg cgctttcaga aaaaccgtga
    55261 gtggtcgggc aactcaattg cgcctggaag atggtcagtg caccgctgac ggtttgaccg
    55321 cagacttcct gcgtgccgcc tgcgtcggta aagcgaatta atgccaccag aatgtgatgc
    55381 agaccaaacg gcaacagcag acgttcaccg gtaccaaaca gcatcggtcc gaaatcaccc
    55441 gcgctattga tcatatggcc taagccgcta atacccatgg cgaaaattgg ccagactaat
    55501 ggaatcacca gaccgacaat gcccatcacc agcgaggaga tgattggtac gaagcgcgta
    55561 ccgccgaaga atgccagcgc atccggcagg cggatattat ggaatcgctc atgcagcatc
    55621 cagacgataa taccggcgat caccgcaccg aggatcccgg tgtcgatcga ctggatccca
    55681 agaatgctct ggatgttatt ggctttcaga acggcggcgt ctgtggttgg cagaatgcct
    55741 ttattggtca accagaagtt taccgcgagg ttcattaccg catatccgac gaagccagcg
    55801 aatgccgcta cgcctttgtt ttcgcgtgcc aggcccagcg ggatggcgat acagaacatg
    55861 acaggcagga aactaaaagc aaacgagcca atcttactca tccaggtaaa gatagcctgc
    55921 aacacggggt tgcccaggac cgggatcagg gttatgacat catggctgct aagagaacta
    55981 ccaatgccga gcataatgcc gcagaacgac aataatgcca cgggtaacat gaaggttttg
    56041 cctaactgct ggaagaactc ccacagcgtg actttcggtg ctgttttcgc cgtcataaaa
    56101 cgactcctcg tagagaacaa tctggattca gtaaattgcg cgagaagata aaacgtttta
    56161 tcaaatttta gtgaggcata aatcacatta cgcaacgata atagcgggta taagataaat
    56221 aaaaggtaaa acgttttacc tgtcacataa tcagggagta ggtcatctgc atggctacag
    56281 ccaaaaaaat aaccattcat gatgttgcgc tggctgcggg cgtgtcggta agtaccgttt
    56341 cgctggtgct tagtggcaaa gggcgaatct ctaccgccac aggagaacgc gttaacgccg
    56401 ccattgaaga gctgggattt gtgcgcaatc gccaggcgtc ggcgctgcgc ggcgggcaaa
    56461 gcggcgtcat tggtttgatc gtccgtgatt tatctgcgcc gttttacgcc gaattgacgg
    56521 ccggattgac ggaagctctg gaagcgcagg gacggatggt ttttttgctt cacggcggta
    56581 aagacggcga gcaactggca cagcggtttt cactgttact gaatcagggt gtcgatggtg
    56641 tggtaattgc cggggctgca ggaagcagcg atgacctgcg acggatggca gaagaaaaag
    56701 ctatcccggt gattttcgct tcccgtgcca gttatcttga tgatgttgat acggttcgcc
    56761 cggacaacat gcaggctgca cagttgttga cggagcatct cattcgcaat gggcatcagc
    56821 ggatcgcctg gctgggaggg caaagttcct cattaacccg agcagaacgg gtggggggct
    56881 attgtgcaac tctactaaaa tttggcctgc cgtttcacag cgattgggtg ttggagtgca
    56941 cttccagcca gaagcaagcc gcggaagcta tcacggcgct tttacgtcat aacccgacca
    57001 tcagtgccgt ggtttgctat aacgaaacta ttgcgatggg ggcatggttt ggtttgctga
    57061 aagcagggcg gcaaagcggg gaaagcggag tcgatcgtta ctttgagcaa caggtttcgc
    57121 tggcggcatt taccgatgcg acaccaacca cacttgatga tatacccgtt acctgggcca
    57181 gcacgcctgc gcgggaactt ggtaccacac ttgcggatcg catgatgcaa aaaatcaccc
    57241 atgaagagac gcattcacgc aatcttatta ttcccgcccg gctcattgcg gcgaaataac
    57301 cctctcttta tctgctatac actggtagtg ttctttcctc aaggttaatg ataaaaaatg
    57361 tggcacacag gcggtgcctt ttatttttac tgaagttcac aggaggttta tgtgtttaat
    57421 tctgacaacc tgagactcga cggaaaatgc gccatcatca caggtgcggg tgcaggtatt
    57481 ggtaaagaaa tcgccattac attcgcgaca gctggcgcat ctgtggtggt cagtgatatt
    57541 aacgccgacg cagctaacca tgttgtagac gaaattcaac aactgggtgg tcaggcattt
    57601 gcctgccgtt gtgatattac ttccgaacag gaactctctg cactggcaga ctttgctatc
    57661 agtaagctgg gtaaagttga tattctggtt aacaacgccg gtggcggtgg acctaaaccg
    57721 tttgatatgc caatggcgga ttttcgccgt gcttatgaac tgaatgtgtt ttcttttttc
    57781 catctgtcac aacttgttgc gccagaaatg gaaaaaaatg gcggtggcgt tattctgacc
    57841 atcacttcta tggcggcaga aaataaaaat ataaacatga cttcctatgc atcatctaaa
    57901 gctgcggcca gtcatctggt cagaaatatg gcatttgacc tgggtgaaaa aaatattcgg
    57961 gtgaatggca ttgcgccggg ggcaatatta accgatgccc tgaaatccgt tattacacca
    58021 gaaattgaac aaaaaatgtt acagcacacg ccgatcagac gtctgggcca accgcaagat
    58081 attgctaacg cagcgctgtt cctttgctcg cctgcagcca gctgggtaag cggacaaatt
    58141 ctcaccgtct ccggtggtgg ggtacaggag ctcaattaat acactaacga accggtaaac
    58201 agccgtatgt gttgtttacc gggatagcgt gtgcaattca tcaacgtctc tgctaaacaa
    58261 ctggcagcca aatcacggct attggttaac caatagctgg atgaaaagca tacgaataga
    58321 gtgtgccttc gcactattca acagcaatga taggcgctca cctgacaacg cggtaaacta
    58381 gttattcacg ctaactataa tggtttaatg atggataaca tgcagactga agcacaaccg
    58441 acacggaccc ggatcctcaa tgctgccaga gagatttttt cagaaaatgg atttcacagt
    58501 gcctcgatga aagccatctg taaatcttgc gccattagtc ccgggacgct ctatcaccat
    58561 ttcatctcca aagaagcctt gattcaggcg attatcttac aggaccagga gagggcgctg
    58621 gcccgtttcc gggaaccgat tgaagggatt cattttgttg actatatggt cgagtctatt
    58681 gtctctctca cccatgaagc ctttgggcaa cgggcgctgg tggtcgaaat tatggcggaa
    58741 gggatgcgta acccacaggt cgccgccatg cttaaaaata agcatatgac gatcactgaa
    58801 tttgttgctc agcggatgcg tgatgcccag caaaaaggcg agataagccc agacatcaac
    58861 acggcaatga cttcacgttt actgctggat ctgacctacg gtgtactggc cgatatcgaa
    58921 gcggaagacc tggcgcgtga agcgtcgttt gctcagggat tacgcgcgat gattggcggt
    58981 atcttaaccg catcctgatt ctctctctct ttccgcgggc tggtgacaac tgaacccgcg
    59041 tttcatactt taatttccct gtacaaaata caatctcgcc ggagcgggga attcctccct
    59101 aaaatattca ccgtagccat atgtcatgag agtttatcgt tcccaatatg ctcgaacgaa
    59161 cgttcggttg cttattttat ggcttctgtc aacgccgttt taaagattaa tgcgatctat
    59221 atcacgctgt gggtattgca gtttttggtt ttttgatcgc ggtgtcagtt ctttttattt
    59281 ccatttctct tccatgggtt tctcacagat aactgtgtgc aatacagaat tggttaacta
    59341 atcagattaa aggttgacca gtatttttat cttaatgagg agtcccttat gttacgtcct
    59401 gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat
    59461 cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg cgttacaaga aagccgggca
    59521 attgctgtgc caggcagttt taacgatcag ttcgccgatg cagatattcg taattatgtg
    59581 ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag gttgggcggg ccagcgtatc
    59641 gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt gggtaaataa tcaggaagtg
    59701 atggagcatc agggcggcta tacgccattt gaagctgatg tcacgccgta tgttattgcc
    59761 gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg
    59821 ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc
    59881 tttaactacg ccgggatcca tcgcagcgta atgctctaca ccacgccgaa cacttgggtg
    59941 gacgatatca ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg
    60001 caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt
    60061 gcaactggac aaggcaccag cgggactttg caagtggtga atccgcacct ctggcaaccg
    60121 ggtgaaggtt atctctatga actgtacgtc acagccaaaa gccagacaga gtgtgatatc
    60181 tacccgctgc gcgtcggcat ccggtcagtg gcagtgaagg gcgaacagtt cctgatcaac
    60241 cacaaaccgt tctactttac tggctttggc cgtcatgaag atgcggattt gcgcggcaaa
    60301 ggattcgata acgtgctgat ggtgcacgat cacgcattaa tggactggat tggggccaac
    60361 tcctaccgta cctcgcatta cccttatgcc gaagagatgc tcgactgggc agatgaacat
    60421 ggcatcgtgg tgattgatga aactgcagct gtcggcttta acctctcttt aggcattggt
    60481 ttcgaagcgg gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa cgggaaaact
    60541 cagcaggcgc acttacaggc gattaaagag ctgattgcgc gtgacaaaaa ccacccaagc
    60601 gtggtgatgt ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc acgggaatat
    60661 ttcgcgccac tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc
    60721 aatgtaatgt tctgcgacgc tcacaccgat accatcagcg atctctttga tgtgctgtgc
    60781 ctgaaccgtt attacggatg gtatgtccaa agcggcgatt tggaaacggc agagaaggta
    60841 ctggaaaaag aacttctggc ctggcaggag aaactgcatc agccgattat catcaccgaa
    60901 tacggcgtgg atacgttagc cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag
    60961 tatcagtgtg catggctgga tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc
    61021 ggtgaacagg tatggaattt cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc
    61081 ggtaacaaga agggcatctt cacccgcgac cgcaaaccga agtcggcggc ttttctgctg
    61141 caaaaacgct ggactggcat gaacttcggt gaaaaaccgc agcagggagg caaacaatga
    61201 atcaacaact ctcctggcgc accatcgttg gctacagcct cggtgacgtc gccaataact
    61261 tcgccttcgc tatgggggcg ctcttcctgc taagttacta caccgacgtc gctggcgtcg
    61321 gtgccgctgc ggcgggcacc atgctgttac tggtgcgggt attcgatgcc ttcgccgacg
    61381 tctttgccgg acgagtggtg gacagtgtga atacccgctg gggaaaattc cgcccgtttt
    61441 tactcttcgg tactgcgccg ttaatgatct tcagcgtgct ggtattctgg gtgccgaccg
    61501 actggagcca tagcagcaaa gtagtgtatg catatttgac ctacatgggc ctcgggcttt
    61561 gctacagcct ggtgaatatt ccttatggtt cacttgctac cgcgatgacc caacaaccac
    61621 aatcccgcgc ccgtctgggc gcggctcgcg ggattgccgc ttcattaacc tttgtctgcc
    61681 tggcatttct gataggaccg agcattaaga actccagccc ggaagagatg gtgtcggtat
    61741 accatttctg gacgattgtg ctggcgattg ccggaatggt gctttacttc atctgcttca
    61801 aatcgacgcg tgagaatgtg gtacgtatcg tggcgcagcc gtcattgaag atcagtctgc
    61861 aaaccctgaa acggaatcgc ccgctgttta tgttgtgcat cggtgcgctg tgtgtgctga
    61921 tttcgacctt cgcggtcagc gcctcgtcgt tgttctacgt gcgctatgtg ttaaatgata
    61981 ccgggctgtt cactgtgctg gtactggtgc aaaacctggt cggtactgtg gcatcggcac
    62041 cgctggtgcc ggggatggtc gcgaggatcg gtaaaaagaa taccttcctg attggcgctt
    62101 tactgggaac ctgcggttat ctgctattct tctgggtttc cgtctggtca ctgccggtgg
    62161 cgttggttgc gttggccatc gcttcaattg gtcagggcgt taccatgacc gtgatgtggg
    62221 cgctggaagc tgataccgta gaatacggtg aatacctgac cggcgtgcga attgaagggc
    62281 tcacctattc actattctca tttacccgta aatgcggtca ggcaatcgga ggttcaattc
    62341 ctgcctttat tttggggtta agcggatata tcgccaatca ggtgcaaacg ccggaagtta
    62401 ttatgggcat ccgcacatca attgccttag taccttgcgg atttatgcta ctggcattcg
    62461 tcattatctg gttttatccg ctcacggata aaaaattcaa agaaatcgtg gttgaaattg
    62521 ataatcgtaa aaaagtgcag
//
LOCUS       DAZFFG010000015        60685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFFG010000015 DAZFFG010000000
VERSION     DAZFFG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.015"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            153..602
                     /gene="alaE"
                     /locus_tag="ACHJ7J_001220"
     CDS             153..602
                     /gene="alaE"
                     /locus_tag="ACHJ7J_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO4999485.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(639..983)
                     /gene="ygaC"
                     /locus_tag="ACHJ7J_001221"
     CDS             complement(639..983)
                     /gene="ygaC"
                     /locus_tag="ACHJ7J_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO4999486.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1135..1464
                     /gene="ygaM"
                     /locus_tag="ACHJ7J_001222"
     CDS             1135..1464
                     /gene="ygaM"
                     /locus_tag="ACHJ7J_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO4999487.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1614..2948)
                     /locus_tag="ACHJ7J_001223"
     CDS             complement(1614..2948)
                     /locus_tag="ACHJ7J_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4999488.1"
                     /translation="MPRYQDIARQLKTAIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGENQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YLDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISHFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3036..3467
                     /locus_tag="ACHJ7J_001224"
     CDS             3036..3467
                     /locus_tag="ACHJ7J_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4999489.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3676..3921
                     /gene="nrdH"
                     /locus_tag="ACHJ7J_001225"
     CDS             3676..3921
                     /gene="nrdH"
                     /locus_tag="ACHJ7J_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO4999490.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3918..4328
                     /gene="nrdI"
                     /locus_tag="ACHJ7J_001226"
     CDS             3918..4328
                     /gene="nrdI"
                     /locus_tag="ACHJ7J_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO4999491.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4301..6445
                     /gene="nrdE"
                     /locus_tag="ACHJ7J_001227"
     CDS             4301..6445
                     /gene="nrdE"
                     /locus_tag="ACHJ7J_001227"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4999492.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFDHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VVDERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYNENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6455..7414
                     /gene="nrdF"
                     /locus_tag="ACHJ7J_001228"
     CDS             6455..7414
                     /gene="nrdF"
                     /locus_tag="ACHJ7J_001228"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4999493.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKRLEKISEAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7770..8972
                     /gene="proV"
                     /locus_tag="ACHJ7J_001229"
     CDS             7770..8972
                     /gene="proV"
                     /locus_tag="ACHJ7J_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO4999494.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKAIDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8965..10029
                     /gene="proW"
                     /locus_tag="ACHJ7J_001230"
     CDS             8965..10029
                     /gene="proW"
                     /locus_tag="ACHJ7J_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO4999495.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTNTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10085..11077
                     /gene="proX"
                     /locus_tag="ACHJ7J_001231"
     CDS             10085..11077
                     /gene="proX"
                     /locus_tag="ACHJ7J_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO4999496.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11269..12453
                     /locus_tag="ACHJ7J_001232"
     CDS             11269..12453
                     /locus_tag="ACHJ7J_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4999497.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12577..13314
                     /gene="ygaZ"
                     /locus_tag="ACHJ7J_001233"
     CDS             12577..13314
                     /gene="ygaZ"
                     /locus_tag="ACHJ7J_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO4999498.1"
                     /translation="MESPTTQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13304..13639
                     /gene="ygaH"
                     /locus_tag="ACHJ7J_001234"
     CDS             13304..13639
                     /gene="ygaH"
                     /locus_tag="ACHJ7J_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO4999499.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13730..14260
                     /gene="emrR"
                     /locus_tag="ACHJ7J_001235"
     CDS             13730..14260
                     /gene="emrR"
                     /locus_tag="ACHJ7J_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO4999500.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14387..15559
                     /gene="emrA"
                     /locus_tag="ACHJ7J_001236"
     CDS             14387..15559
                     /gene="emrA"
                     /locus_tag="ACHJ7J_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO4999501.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQTFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15576..17114
                     /gene="emrB"
                     /locus_tag="ACHJ7J_001237"
     CDS             15576..17114
                     /gene="emrB"
                     /locus_tag="ACHJ7J_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO4999502.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17178..17693)
                     /gene="luxS"
                     /locus_tag="ACHJ7J_001238"
     CDS             complement(17178..17693)
                     /gene="luxS"
                     /locus_tag="ACHJ7J_001238"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO4999503.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17843..19399)
                     /gene="gshA"
                     /locus_tag="ACHJ7J_001239"
     CDS             complement(17843..19399)
                     /gene="gshA"
                     /locus_tag="ACHJ7J_001239"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO4999504.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEFAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19472..19900)
                     /gene="yqaA"
                     /locus_tag="ACHJ7J_001240"
     CDS             complement(19472..19900)
                     /gene="yqaA"
                     /locus_tag="ACHJ7J_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO4999505.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19897..20463)
                     /gene="yqaB"
                     /locus_tag="ACHJ7J_001241"
     CDS             complement(19897..20463)
                     /gene="yqaB"
                     /locus_tag="ACHJ7J_001241"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO4999506.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20744..20820)
                     /locus_tag="ACHJ7J_001242"
     tRNA            complement(20744..20820)
                     /locus_tag="ACHJ7J_001242"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20784..20786),aa:Arg,seq:acg)
     gene            complement(21018..21094)
                     /locus_tag="ACHJ7J_001243"
     tRNA            complement(21018..21094)
                     /locus_tag="ACHJ7J_001243"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21058..21060),aa:Arg,seq:acg)
     gene            complement(21157..21233)
                     /locus_tag="ACHJ7J_001244"
     tRNA            complement(21157..21233)
                     /locus_tag="ACHJ7J_001244"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21197..21199),aa:Arg,seq:acg)
     gene            complement(21298..21374)
                     /locus_tag="ACHJ7J_001245"
     tRNA            complement(21298..21374)
                     /locus_tag="ACHJ7J_001245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21338..21340),aa:Arg,seq:acg)
     gene            complement(21378..21470)
                     /locus_tag="ACHJ7J_001246"
     tRNA            complement(21378..21470)
                     /locus_tag="ACHJ7J_001246"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21434..21436),aa:Ser,seq:gct)
     gene            complement(21786..21971)
                     /gene="csrA"
                     /locus_tag="ACHJ7J_001247"
     CDS             complement(21786..21971)
                     /gene="csrA"
                     /locus_tag="ACHJ7J_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO4999507.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(22206..24836)
                     /gene="alaS"
                     /locus_tag="ACHJ7J_001248"
     CDS             complement(22206..24836)
                     /gene="alaS"
                     /locus_tag="ACHJ7J_001248"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO4999508.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24964..25464)
                     /gene="recX"
                     /locus_tag="ACHJ7J_001249"
     CDS             complement(24964..25464)
                     /gene="recX"
                     /locus_tag="ACHJ7J_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO4999509.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25707..26768)
                     /gene="recA"
                     /locus_tag="ACHJ7J_001250"
     CDS             complement(25707..26768)
                     /gene="recA"
                     /locus_tag="ACHJ7J_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO4999510.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26848..27345)
                     /gene="pncC"
                     /locus_tag="ACHJ7J_001251"
     CDS             complement(26848..27345)
                     /gene="pncC"
                     /locus_tag="ACHJ7J_001251"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO4999511.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27490..28575)
                     /gene="mltB"
                     /locus_tag="ACHJ7J_001252"
     CDS             complement(27490..28575)
                     /gene="mltB"
                     /locus_tag="ACHJ7J_001252"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO4999512.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGMPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28831..29394
                     /gene="srlA"
                     /locus_tag="ACHJ7J_001253"
                     /gene_synonym="gutA"
     CDS             28831..29394
                     /gene="srlA"
                     /locus_tag="ACHJ7J_001253"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO4999513.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            29391..30350
                     /gene="srlE"
                     /locus_tag="ACHJ7J_001254"
                     /gene_synonym="gutE"
     CDS             29391..30350
                     /gene="srlE"
                     /locus_tag="ACHJ7J_001254"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO4999514.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLVQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLSNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            30361..30732
                     /gene="srlB"
                     /locus_tag="ACHJ7J_001255"
                     /gene_synonym="gutB"
     CDS             30361..30732
                     /gene="srlB"
                     /locus_tag="ACHJ7J_001255"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO4999515.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30736..31515
                     /gene="srlD"
                     /locus_tag="ACHJ7J_001256"
     CDS             30736..31515
                     /gene="srlD"
                     /locus_tag="ACHJ7J_001256"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO4999516.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31621..31980
                     /gene="gutM"
                     /locus_tag="ACHJ7J_001257"
     CDS             31621..31980
                     /gene="gutM"
                     /locus_tag="ACHJ7J_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO4999517.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFAKPQKIPAITGMYPGDLRPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            32047..32820
                     /gene="srlR"
                     /locus_tag="ACHJ7J_001258"
                     /gene_synonym="gutR"
     CDS             32047..32820
                     /gene="srlR"
                     /locus_tag="ACHJ7J_001258"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO4999518.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVMLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            32813..33778
                     /gene="gutQ"
                     /locus_tag="ACHJ7J_001259"
     CDS             32813..33778
                     /gene="gutQ"
                     /locus_tag="ACHJ7J_001259"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO4999519.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGNDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLVGAINLQDFYQAGII"
     gene            complement(33775..35289)
                     /gene="norR"
                     /locus_tag="ACHJ7J_001260"
     CDS             complement(33775..35289)
                     /gene="norR"
                     /locus_tag="ACHJ7J_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO4999520.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35476..36915
                     /gene="norV"
                     /locus_tag="ACHJ7J_001261"
     CDS             35476..36915
                     /gene="norV"
                     /locus_tag="ACHJ7J_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO4999521.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            36912..38045
                     /gene="norW"
                     /locus_tag="ACHJ7J_001262"
     CDS             36912..38045
                     /gene="norW"
                     /locus_tag="ACHJ7J_001262"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO4999522.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGIQA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(38275..40527)
                     /gene="hypF"
                     /locus_tag="ACHJ7J_001263"
     CDS             complement(38275..40527)
                     /gene="hypF"
                     /locus_tag="ACHJ7J_001263"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO4999523.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMMRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLEGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRVFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40680..41207)
                     /gene="hydN"
                     /locus_tag="ACHJ7J_001264"
     CDS             complement(40680..41207)
                     /gene="hydN"
                     /locus_tag="ACHJ7J_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO4999524.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(41356..42366)
                     /gene="ascG"
                     /locus_tag="ACHJ7J_001265"
     CDS             complement(41356..42366)
                     /gene="ascG"
                     /locus_tag="ACHJ7J_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO4999525.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            42626..44083
                     /gene="ascF"
                     /locus_tag="ACHJ7J_001266"
     CDS             42626..44083
                     /gene="ascF"
                     /locus_tag="ACHJ7J_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO4999526.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEIVSLLPGDMHPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            44092..45516
                     /gene="ascB"
                     /locus_tag="ACHJ7J_001267"
     CDS             44092..45516
                     /gene="ascB"
                     /locus_tag="ACHJ7J_001267"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO4999527.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(45675..46145)
                     /gene="hycI"
                     /locus_tag="ACHJ7J_001268"
     CDS             complement(45675..46145)
                     /gene="hycI"
                     /locus_tag="ACHJ7J_001268"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO4999528.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(46138..46548)
                     /gene="hycH"
                     /locus_tag="ACHJ7J_001269"
     CDS             complement(46138..46548)
                     /gene="hycH"
                     /locus_tag="ACHJ7J_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO4999529.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46545..47312)
                     /gene="hycG"
                     /locus_tag="ACHJ7J_001270"
     CDS             complement(46545..47312)
                     /gene="hycG"
                     /locus_tag="ACHJ7J_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO4999530.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(47312..47854)
                     /gene="hycF"
                     /locus_tag="ACHJ7J_001271"
     CDS             complement(47312..47854)
                     /gene="hycF"
                     /locus_tag="ACHJ7J_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO4999531.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47864..49573)
                     /gene="hycE"
                     /locus_tag="ACHJ7J_001272"
     CDS             complement(47864..49573)
                     /gene="hycE"
                     /locus_tag="ACHJ7J_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO4999532.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPCGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(49591..50514)
                     /gene="hycD"
                     /locus_tag="ACHJ7J_001273"
     CDS             complement(49591..50514)
                     /gene="hycD"
                     /locus_tag="ACHJ7J_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO4999533.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLTLCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(50517..52343)
                     /gene="hycC"
                     /locus_tag="ACHJ7J_001274"
     CDS             complement(50517..52343)
                     /gene="hycC"
                     /locus_tag="ACHJ7J_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO4999534.1"
                     /translation="MSAISLINSGVAWFVAAAILAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLSMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQREGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(52340..52951)
                     /gene="hycB"
                     /locus_tag="ACHJ7J_001275"
     CDS             complement(52340..52951)
                     /gene="hycB"
                     /locus_tag="ACHJ7J_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO4999535.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(53076..53537)
                     /gene="hycA"
                     /locus_tag="ACHJ7J_001276"
     CDS             complement(53076..53537)
                     /gene="hycA"
                     /locus_tag="ACHJ7J_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO4999536.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53749..54099
                     /gene="hypA"
                     /locus_tag="ACHJ7J_001277"
     CDS             53749..54099
                     /gene="hypA"
                     /locus_tag="ACHJ7J_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4999537.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPLCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            54103..54975
                     /gene="hypB"
                     /locus_tag="ACHJ7J_001278"
     CDS             54103..54975
                     /gene="hypB"
                     /locus_tag="ACHJ7J_001278"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO4999538.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     TPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54966..55238
                     /gene="hypC"
                     /locus_tag="ACHJ7J_001279"
     CDS             54966..55238
                     /gene="hypC"
                     /locus_tag="ACHJ7J_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO4999539.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            55238..56359
                     /gene="hypD"
                     /locus_tag="ACHJ7J_001280"
     CDS             55238..56359
                     /gene="hypD"
                     /locus_tag="ACHJ7J_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO4999540.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            56356..57366
                     /gene="hypE"
                     /locus_tag="ACHJ7J_001281"
     CDS             56356..57366
                     /gene="hypE"
                     /locus_tag="ACHJ7J_001281"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO4999541.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLIAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            57440..59518
                     /gene="flhA"
                     /locus_tag="ACHJ7J_001282"
     CDS             57440..59518
                     /gene="flhA"
                     /locus_tag="ACHJ7J_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO4999542.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            59563..59991
                     /locus_tag="ACHJ7J_001283"
     CDS             59563..59991
                     /locus_tag="ACHJ7J_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO4999543.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(60030..60374)
                     /gene="ygbA"
                     /locus_tag="ACHJ7J_001284"
     CDS             complement(60030..60374)
                     /gene="ygbA"
                     /locus_tag="ACHJ7J_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO4999544.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(60406..60585)
                     /locus_tag="ACHJ7J_001285"
     CDS             complement(60406..60585)
                     /locus_tag="ACHJ7J_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999545.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     EPFFFRVIVPFKIKL"
BASE COUNT        14349 a        16427 c        15673 g        14236 t
ORIGIN      
        1 ttttatctac aaaaactgac taaataaaaa tttttcacta attgattagt catagccagc
       61 gatatacgct atgcaaaaat gcagatggca atgagatcca ctgctttcat ctccattaac
      121 atcccattac gcttttatta aggagcatta gcatgttctc accgcagtca cgcttgcgtc
      181 atgcagttgc agatacgttc gcgatggttg tttactgttc tgtcgtgaac atgtgtattg
      241 aagttttcct ctccggaatg agcttcgaac agtcttttta ttccagattg gtagcgattc
      301 cggtgaacat cttaattgca tggccatacg gtatgtaccg cgatctgttt atgcgcgcgg
      361 cacgcaaagt tagcccgtcg ggctggataa aaaatctggc ggatatcctg gcttatgtga
      421 cgttccagtc accggtgtat gtggcgatct tgttagtggt gggcgcagac tggcatcaga
      481 ttatggcggc ggtcagttca aacatcgttg tttcgatgtt gatgggggcg gtttatggct
      541 acttcctcga ttattgccgc cgactgttta aagtcagccg ttaccagcag gtaaaagcct
      601 gactcttctt ttcgcgactg gcatcgccag tcgcgtcttt aactggcttc gccaaataaa
      661 ccattcaaat aacgttcaag cgcaacacgc gaactaaagc catgcgggat accgtaatgc
      721 tcgtgtcgct cgcctgctaa atagagcgga aattgttgat aatgatcgca agttttaata
      781 tcggaagcgg gttcagccag tgtcgaacta cgttctttta atgttggatg aattaccagt
      841 gcggtacgtc ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa
      901 ccatacgctt tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc
      961 acctcgtctg gtcgtaaata catatttgat cctcgttatt atcaaccgcg ggcctacctt
     1021 acctgattgc gcattcacaa cgctttcaga aaagtccata aagccgcgat gaatctcagt
     1081 taagaaaatt atggtctaca ctgaaaatta catcgaattc taatggggga tcatatgttt
     1141 aaccgaccga accgcaacga cgttgatgat ggcgtgcagg atatccagaa tgatgtcaat
     1201 caattagctg acagcctcga atctgtattg aagtcctggg gcagcgacgc caaaggggaa
     1261 gctgaagccg cacgcagtaa agctcaggca ttgctgaaag aaaccagggc gcgaatgcat
     1321 ggtcgtactc gcgtccagca agccgcacgc gatgccgttg gctgcgcgga ttcttttgtt
     1381 cgtgaaagac cctggtgtag cgtgggtaca gcagctgcgg taggtatttt tatcggcgca
     1441 ctgttaagca tgcgcaaatc gtagtgagac gatgacaagc agtagtctga cttctgaaaa
     1501 gtactcagtg cgtcaacgcc ttgtccgcct acaggcctga aaaacatagc gcatcagggg
     1561 attttgccct ggtcaccagt cttataggac cccgaagcct ggcttcgggg ttattaattt
     1621 aaagcactgc gcaattgctg tgctacctgt cgcgccatac tttcgctaac gatattcgta
     1681 aacgagagca ataatcctcc gtcctcgctt gagcggatac gccagtcgct aagtgcctgt
     1741 acggcaagac cagcagcatt ggctttatgc acgaatgtca cgtcattacc tgctagtttg
     1801 atcaccatct ggataccgcc tttttgcggc acaacctgaa atccctgctg cgtaagtgcc
     1861 tgctcaatcc actgccgtcg ctgggcataa tgctggcgca ttttcttcag atgccgccag
     1921 aaatgcccct cacggatgaa atctgccagt gtgttctgcc acagtacggg tacagcacat
     1981 ggttccagtg acgcctgctg ccggaaatgc gaaatctgct ccaccggcac caccagccac
     2041 gcacagcgca atgcaggaaa tagcgctttg ctgaatgttc ctgcataaat cacccgctgc
     2101 ggcgcgtcga gactttttag tgccggtaac ggcttaccgt gatagcgaaa ctcactgtcg
     2161 taatcatctt caataatcca tgcctgacta cgctctgccc attccagtat ctgataccta
     2221 cgcgctaaag agagtgccac acccagcggg ctttggtgtg ccggagtaat cagggcaaaa
     2281 cgcgcatcaa gataattttg tattccgctt gtgacatcca gtccgttgtc atcaaccggc
     2341 acaggtaaaa tttccacacc gtggcgagtg acaatcgggc gaatcagcgg aaagccggga
     2401 tcttccatcc acatcccgtc tcccggttgc gccagagcgt gcagaattaa agccattgag
     2461 gccgcataac cgtgagtgat aaagacctgc tctggctgac aatcaatccc acgtgaaacg
     2521 cgtaaataat caacaatagc ctcgcgcaag gccgcctcgc cgcagacatc gcctaatgcc
     2581 aaatcaaaac gcgtctgggt acgcagacgg cggcccatca cccgcgccca caattcacgc
     2641 ggaaaaagat caagtgcagg tagccccatc tgaaaaggca gcggctgctg attttcacca
     2701 gcaaaagcca cgacatccac agcagactgt tgcggatgta tctgctcact gacaaaggta
     2761 cccgcctgtc cccgcctgac cagccatccc tgcgccacca gctcagcata agcattttcg
     2821 acagtggatc gagagacacc cagctcctgc gaccaggtac ggctcgaagg tagccttgcg
     2881 ccaggtttca gttttccttg ctcgatagcc gtttttaact gacgggcgat atcctgataa
     2941 cgtggcatat ggtctgcttt tttaggtaat ttatggctct ctttagcagt ccattatagt
     3001 gctacattgc gggcgttaat aagaggagaa actgtatgac tacattacgt cagccttact
     3061 acgaacttag cccggcagtg tataacgcac tggtgcaggc caaaacggca ctggaaaata
     3121 gcacgctgga taccacgttg atggagttaa tttatttgcg cgtctcgcaa atcaacggct
     3181 gcgcattttg tctggagatg cacagcaaag cattgcgcaa atccggtgtg ccacaacaca
     3241 aactggacgc cctggcaggc tggcgcgtaa gccatcattt tgatgaacgc gagcgagcgg
     3301 cgctggcatg ggcggaatca gtgaccgata ttgccagaac ccatgcggaa gacgaggttt
     3361 atcagccttt gcttgagcat ttcagcgcag cagaaatcag cgacttaacg tttgccatcg
     3421 ggctgatgaa ttgttttaac cgtctggccg tttccatgcg gatgtaattt gctgttgccg
     3481 ggtatgcgcc cggcaaaaaa acacaaaatc ccctaccccg ttgagccatt caccggtggc
     3541 gtaatcaatc taaaattccc atatcttgtg tggttgaatc ttttttcaac tacatctagt
     3601 atctctgtat caacagagag acaacccgac gcgtatcatc gcgccgtatc ttcattttaa
     3661 acggaaatac gaatcatgcg cattactatt tacactcgta acgattgcgt tcagtgccac
     3721 gccaccaaac gggcgatgga aaaccggggc tttgattttg aaatgattaa tgtcgatcgc
     3781 gttcctgaag cggcagaagc gttgcgtgct cagggctttc gtcagttgcc ggtagtgatt
     3841 gctggcgatc ttagctggtc tggtttccgt ccggacatga ttaaccgtct gcatccagcg
     3901 ccacacgcgg ccagtgcatg agccagctcg tctacttctc cagcagctcc gaaaacacgc
     3961 agcgttttat cgaacgttta ggtctgcccg cggtgcgcat cccgctcaat gagcgggaac
     4021 ggattcaggt agacgagcct tacatcctga tcgtgccctc ttacggcggc ggcggtacgg
     4081 ctggcgcggt gccacgacag gtaattcgct ttttaaacga cgagcacaac cgggcgttgc
     4141 ttcgcggcgt tattgcttcg ggtaatcgca actttggtga ggcgtatggc cgcgctggag
     4201 atgtgattgc ccggaaatgc ggcgtgccgt ggctgtaccg ctttgaactc atgggtacgc
     4261 aaagcgatat cgaaaacgtt cgtaaaggag taaccgaatt ttggcaacga caaccgcaga
     4321 atgcctgacg caggaaacga tggattacca cgcgctgaat gcgatgctta acctctacga
     4381 tagcgcaggt cgcattcagt tcgataaaga ccgccaggcc gttgacgcct ttattgcgac
     4441 gcatgtgcgt ccgaacagtg tgaccttcag tagccagcag cagcgcctga actggctggt
     4501 caacgaaggt tactatgatg aaagcgttct taatcgctac tctcgcgact ttgtcattac
     4561 gctgtttgac cacgcacaca ccagcggttt tcgtttccag acattcctcg gggcatggaa
     4621 gttttacacc agctatacgt tgaagacatt cgacggtaaa cgttatctgg aagattttgc
     4681 cgatcgagtc acgatggtgg cgctgacgct ggcacaaggc gatgagacgc tggcgttgca
     4741 actgacagat gaaatgctgt caggacgctt tcagccagcc acgccaacat tcctcaactg
     4801 cggtaagcag cagcgcggcg aactggtttc ctgttttttg ctgcgtattg aagacaatat
     4861 ggagtcgatt ggtcgggcgg taaattccgc actgcaactg tcgaaacgcg gcggcggcgt
     4921 agcatttttg ctgtcgaatc tgcgggaagc gggcgcgcca attaaacgta ttgaaaatca
     4981 atcttctggc gtaattccgg tgatgaaaat gctggaagac gcattttcct atgccaacca
     5041 actcggcgcg cgtcaggggg ctggggcagt ctatttacat gctcatcatc ccgatattct
     5101 gcgttttctc gacacgaaac gggaaaatgc cgacgaaaaa atccgcatta aaacactgtc
     5161 gcttggcgtg gtgatcccgg atatcacttt ccatctggca aaagagaatg cgcagatggc
     5221 gctgttttcg ccttatgacg tagagcgagt ttatggcaag ccgtttgccg atgtggccat
     5281 cagtcaacac tatgacgaac tggttgtcga tgaacgcatt cgaaaaaaat acctcaacgc
     5341 ccgtgatttc ttccagcgac tggcagaaat ccagtttgag tccggctatc cgtacatcat
     5401 gtatgaagac acggtaaacc gtgctaaccc tatcgccggg cgcataaata tgagtaatct
     5461 ctgctcagag attttgcagg ttaacagcgc ctcagagtat aacgagaatc tcgactatgc
     5521 ccgcacaggc catgatatct cctgcaattt aggttcgttg aatattgccc acaccatgga
     5581 ttcccccgac tttgcccgca cggtagagac tgccgtgcgc ggtttaacgg cagtatcaga
     5641 tatgagtcat atccgcagcg tgccgtccat cgaagccgga aatgccgcct cgcacgccat
     5701 cggactgggg cagatgaatt tacatggtta tctggcgagg gaaggcatcg cttatggttc
     5761 gccggaagca ctggatttca ccaatctcta tttctatacc atcacctggc acgcactgcg
     5821 tacctcgatg ttgctggcgc gcgaacgcgg tgaaaccttc gccgggttca aacagtcacg
     5881 ctatgccagc ggtgaatatt ttagccaata tctgcaaggt aactggcagc cgaaaaccgc
     5941 gaaagttggc gaactgtttg cccgcagcgg tattacgtta cctacccgtg agatgtgggc
     6001 gcagctgcgc gatgacgtga tgcgctacgg catttacaac cagaatctcc aggcggtgcc
     6061 gccaaccggt tctatctctt atatcaacca tgctacgtcg agtattcatc cgattgtggc
     6121 gaaagtagag atacgcaaag agggcaaaac aggacgcgtt tactaccctg ccccgtttat
     6181 gactaacgag aatctggcgc tgtatcagga cgcttacgaa attggcgcag aaaagatcat
     6241 cgacacctac gcggaagcga ctcgccatgt cgatcagggg ctgtcgctga cgctgttttt
     6301 ccccgatacc gccaccactc gcgatatcaa caaagcgcag atttacgcct ggcgcaaagg
     6361 tatcaaaacg ctctattaca tccgcctgcg tcagatggcg ctggaaggca ctgaaattga
     6421 aggctgcgtc tcctgtgcac tttaaggaat atctatgaaa ctctcacgta tcagcgccat
     6481 caactggaac aagatatctg acgataaaga tctggaggtg tggaatcgcc tgaccagcaa
     6541 tttctggcta ccagaaaagg tgccactgtc gaacgatata cctgcatggc agacattaag
     6601 tgtcgtagaa caacaactga caatgcgcgt ttttactggc ctgacgctgc tcgacacgct
     6661 gcaaaatgtt atcggcgcgc cttcgctgat gcccgatgcg ctcacgcccc atgaagaggc
     6721 ggtattatcg aatatcagct ttatggaagc ggttcatgcc cgttcttaca gttcgatttt
     6781 ctcgacgttg tgccagacca aagatgtcga tgccgcctac gcctggagtg aagaaaacgc
     6841 accgttgcag cgaaaagctc agattattca gcaacattat cgcggtgatg atccgctgaa
     6901 aaagaaaatc gccagtgtgt ttcttgaatc ttttttgttc tattccggtt tctggctgcc
     6961 gatgtatttt tccagccgcg gaaagctgac caataccgcg gacctgatcc gtctgattat
     7021 ccgcgatgaa gcagtccacg gttactacat aggctataaa tatcagaaaa gactggaaaa
     7081 gatctccgag gcacaacgtg aagagttaaa gagtttcgcc ttcgatttgt tgctagaact
     7141 ctatgacaac gagttgcaat ataccgatga gctgtacgcc gaaaccccgt gggctgacga
     7201 tgtgaaagcg tttctctgtt acaacgccaa taaggcgttg atgaatctgg gctacgaacc
     7261 tttatttccc gcagaaatgg cggaagtgaa tccggcaatc ctcgccgcgc tttcgccgaa
     7321 tgccgatgaa aatcacgatt tcttttccgg ttcaggctcc tcttatgtga tggggaaagc
     7381 ggttgaaaca gaagatgaag actggaattt ctgagggtgt tattttcaaa aatatcacta
     7441 cccgcgacag ggaaataatt cccgccaaat agctttttat cacgcaaata ttttgtggtg
     7501 atctacactg atactctgtt gcattattcg cctgaaacca caatattcag gcgttttttc
     7561 gctatctttg acaaaaaata tcaacttttc tcgatttgct ctcagccctt atatcacggg
     7621 aaattccggc gatttgctca catcaatatt catgccacat ttgccctcag gggttgcctc
     7681 agattctcag tatgttaggg tagaaaaaag tgactatttc cattgggtaa tgtatcgaca
     7741 taaacaaata acaggaatct ttctattgca tggcaattaa attagaaatt aaaaatcttt
     7801 ataaaatatt tggtgagcat ccacagcgag ctttcaaata tatcgaacaa ggactttcaa
     7861 aagaacaaat tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca
     7921 ttgaagaagg cgagatattt gtcatcatgg gattatccgg ttcgggtaaa tccacaatgg
     7981 tacgccttct caatcgcctg attgaaccca cccgcgggca agtactgatt gatggtgtgg
     8041 atattgccaa aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg
     8101 tcttccagtc ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgtttggta
     8161 tggaattggc cggaattaat gccgaagaac gccgggaaaa agccattgat gcactgcgtc
     8221 aggtcgggct ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc
     8281 aacgtgtggg attagcccgc gcgttagcga ttaatccgga tattttatta atggatgaag
     8341 ccttctcggc gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac
     8401 aggcgaaaca tcagcgcacc attgtcttta tttcccacga tctcgatgaa gccatgcgta
     8461 ttggcgaccg aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg
     8521 aaattctcaa taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta
     8581 gtcaggtatt cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa
     8641 cccctggctt cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg
     8701 gctacgttat cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa
     8761 ccgcgttaac gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg
     8821 atgcacaaac gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc
     8881 ccgtggtcga cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg
     8941 ctttagatcg tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca
     9001 gcggcggaca gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccgact
     9061 gacggcggtg gtgctgactg gctgaccaat acgcctgcgc caaacgtcga gcattttaat
     9121 attctcgatc cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc
     9181 gactgggtcg ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat
     9241 atcctcaacg gtttccagca attgctgctg ggtatgcccg cgccggtggc gattatcgtt
     9301 ttcgctctca tcgcctggca gatttccggg gtcggaatgg gcgtggcgac gctggtttcg
     9361 ctgattgcca tcggcgcaat cggtgcctgg tcgcaggcaa tggttactct ggcgctggtg
     9421 ttaaccgccc tgctgttctg tatcgtcatc ggtttgccgt tggggatctg gctggcgaga
     9481 agtccgcgag cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg
     9541 tttgtttatc tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg
     9601 gtgacgatca tctttgctct gccgccgatt atccgtctga ccattctggg gattaaccag
     9661 gttccggcgg atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg
     9721 ttcaaagttc agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg
     9781 atgctggccc tttctatggt ggtcatcgcc tcgatgattg ccgtcggcgg gttgggtcag
     9841 atggtacttc gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg
     9901 attgtgatcc tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc
     9961 agtcgcggca accgtcgctg gtacaccact ggccctgtcg gtctgctgac ccgcccattc
    10021 attaagtaac tctgcacttg cccggtgacg ccgggcatat caccctgcca aaaaaggaat
    10081 aacaatgcga catagcgtac tttttgcgac agcgtttgcc acgcttatct ctacacaaac
    10141 ttttgctgcc gatctgccgg gcaaaggcat tactgttaat ccagttcaaa gcaccatcac
    10201 cgaagaaacc ttccagacac tgctggtcag tcgtgcgctg gagaaattag gttataccgt
    10261 caataaaccc agcgaagtgg attacaacgt tggctacacc tcgcttgctt ccggcgatgc
    10321 aaccttcacc gccgtgaact ggacaccact gcatgacaac atgtacgaag ctgccggtgg
    10381 cgataagaaa ttttatcgtg aaggggtatt tgttaacggc gcggcacagg gttacctgat
    10441 cgataagaaa accgccgacc agtacaaaat caccaacatc gcacaactaa aagatccgaa
    10501 gatcgccaaa ctgttcgata ccaacggcga cggtaaagcg gatttgactg gctgtaaccc
    10561 tggctggggc tgcgaagggg cgatcaacca ccagcttgcc gcgtatgaac tgaccaatac
    10621 cgtgacgcat aatcagggga actacgcggc gatgatggct gacaccatca gtcgttacaa
    10681 agagggcaaa ccggtgtttt actacacctg gacgccgtac tgggtgagta atgagctgaa
    10741 gccagggaaa gatgtggtct ggttgcaggt gccgttctct gcactgccgg gcgataaaaa
    10801 tgccgatacc aaactgccga atggcgcgaa ttacggcttc ccggtcagca ccatgcatat
    10861 cgttgccaat aaagcctggg ccgagaaaaa cccggcagca gcgaaactgt ttgccattat
    10921 gcagttgcca gtggcagata ttaacgccca gaacgccatt atgcatgacg gcaaagcctc
    10981 agaaggcgat attcagggcc acgttgatgg ctggatcaaa gcccaccagc aacagttcga
    11041 tggctgggtg aatgaggcgt tggcagcgca gaagtaattt ttattcgggc ggataaggcg
    11101 tttacgccgc atccgccgtt ctgtacacaa tgcctgatgc gacgctggcg cgtcttatca
    11161 tgcctacaaa ccacaccgca catttcaaca atctatcttt catcccatat tcatcaacat
    11221 ccgctattat tgatttccag cttaatcatc acctgatgaa caaaaataat gaccaaacct
    11281 aatcatgagc ttagcccggc gctaatcgtg ctgatgtcta tcgccaccgg tctggcggta
    11341 gccagtaact attacgccca gccattgctc gacaccatcg cgcgtaactt ttccctttcc
    11401 gccagttcgg caggctttat tgttaccgcc gcgcagttgg gctatgccgc aggtctactg
    11461 tttcttgttc ccctcggtga tatgtttgaa cgccgccgcc tgattgtctc gatgacctta
    11521 ctggcggcat gcggcatgtt gattaccgcc agcagtcagt cgctggcgat gatgatcctc
    11581 ggtacggcat taaccggttt attctcagtc gtggcacaaa ttctggttcc actggcagcg
    11641 acgctggctt caccggataa acgcggcaaa gtggttggca ccattatgag cggcctgctg
    11701 ttggggatat tgctggcacg aaccgttgcc gggttactgg caaacctcgg cggctggcgc
    11761 accgtctttt gggttgcctc ggtgttaatg gctctgatgg cgctagcatt atggcgtggt
    11821 ctgccacaaa tgaaatcaga aacccacctc aattacccac agttgttggg ttccgttttc
    11881 agtatgttta tcagcaataa gatcctgcgc acccgcgcgt tgctgggctg cctgaccttt
    11941 gccaacttca gcattctctg gacctcaatg gcctttttgc ttgccgcacc gccttttaac
    12001 tacagcgatg gtgtaattgg tctgtttggg cttgcgggag ctgccggagc gttgggcgct
    12061 cgtccggcgg gcggttttgc cgacaagggc aaatcgcacc acaccacaac attcggtctg
    12121 cttctgctgt tactttcatg gctggcaatc tggtttggtc acacttccgt actggcgttg
    12181 attatcggca ttctggtgct ggacctcacc gtacagggcg tgcatatcac taaccagacg
    12241 gtaatttatc gaatacatcc tgatgcgcgt aatcgcctga ccgcaggtta catgaccagc
    12301 tactttattg gcggtgccgc cggttcgcta atttcagcct cagcctggca acatggcggt
    12361 tgggctggcg tttgtctggc tggcgcgacg attgccctgg ttaacttact ggtctggtgg
    12421 cgaggttttc atcgtcagga agccgcaaat taagcaatca gcaaccgttt tcgtggcttt
    12481 acacttataa gggtgttaag aagcccatca gtctgataag gttaagatat tcattcagtc
    12541 tatttataat attaacaatc gttaagcgta cactctatgg aaagccctac tacacagcct
    12601 gctcctggtt cggcgacctt catggaagga tgcaaagaca gtttaccgat tgttattagt
    12661 tatattccgg tggcctttgc gttcggtctg aatgcgaccc gtctgggatt ctctcctctc
    12721 gaaagcgttt ttttctcctg catcatttat gcaggcgcga gccagttcgt cattaccgcg
    12781 atgctggcag ccgggagtag tttgtgggtt gctgcactga ccgtcatggc aatggatgtt
    12841 cgccatgtgt tgtatggccc gtcactgcgt agccgtatta ttcagcgtct gcaaaaatcg
    12901 aaaaccgccc tgtgggcgtt tggcctgacg gatgaggttt ttgccgccgc aaccgcaaaa
    12961 ctggtacgca ataatcgccg ctggagcgag aactggatga tcggcattgc cttcagttca
    13021 tggtcatcgt gggtatttgg tacggtaata ggggcattct ccggcagcgg cttgctgcaa
    13081 ggttatcccg ccgttgaagc agcattaggt tttatgcttc cggcactctt tatgagtttc
    13141 ctgctcgcct ctttccagcg caaacaatct ctttgcgtta ccgcagcgtt agttggtgcc
    13201 cttgcaggcg taacgctatt ttctattccc gtcgccattc tggcaggcat tgtctgtggc
    13261 tgcctcactg cgttaatcca ggcattctgg caaggagcgc ccgatgagct atgaggttct
    13321 gctgcttggg ttactggttg gcgcggcgaa ttattgcttc cgctatttgc cgctgcgcct
    13381 gcgtgtgggt aatgcccgcc caaccaaacg tggcgcggta ggtattttgc tcgacaccat
    13441 tggcatcgcc tcgatatgcg ctctgctggt tgtctctacc gcaccagaag tgatgcacga
    13501 tacacgccgt ttcgtgccca cgctggtcgg cttcgcggta ctgggtgcca gtttctataa
    13561 aacacgcagc attatcatcc caacactgct tagtgcgctg gcctatgggc tcgcctggaa
    13621 agtgatggcg attatataac ccacaagaat catttttcta aaacaataca tttactttat
    13681 ttgtcactgt cgttactata tcggctgaaa ttaatgaggt catacccaaa tggatagttc
    13741 gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc cgccacgaag attttcctta
    13801 tcaggagatc cttctgactc gtctttgcat gcacatgcaa agcaagctgc tggagaaccg
    13861 caataaaatg ctgaaggctc aggggattaa cgagacgttg tttatggcgt tgattacgct
    13921 ggagtctcag gaaaaccaca gtattcagcc ttctgaatta agttgtgctc ttggatcatc
    13981 ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa cgcggttgga tcgaacgtcg
    14041 tgaaagcgat aacgatcgcc gctgcctgca tctgcaatta acggaaaaag gtcacgagtt
    14101 tttgcgcgag gttttaccac cgcagcataa ctgcctgcat caactctggt ccgcgctcag
    14161 cacaacagaa aaagatcagc tcgagcaaat cacccgcaaa ttgctctccc gtctcgacca
    14221 gatggaacaa gacggtgtgg ttctcgaagc gatgagctaa cgcgtcatct cgctcaaaaa
    14281 tccagattta taaaagaaaa aatgaccggc cagcatcgca acatgctggc ctttttggca
    14341 agcaggtcgg ctcagccgat gagttaagaa gatcgtggag aacaatatga gcgcaaatgc
    14401 ggagactcaa accccgcagc aaccggtaaa gaagagcggc aaacgtaagc gtctgctcct
    14461 ccttctcacc ttgctcttta taattattgc cgtagcgata gggatttatt ggtttttagt
    14521 actgcgtcat ttcgaagaaa ccgatgacgc atacgtggca gggaatcaaa ttcaaattat
    14581 gtctcaggtg tctggcagcg tgacgaaagt ctgggccgat aacaccgatt ttgtaaaaga
    14641 aggcgacgtg ctggtcactc tcgacccgac agatgctcgc cagacgtttg aaaaagccaa
    14701 aactgcactg gcttccagcg ttcgccaaac ccaccagctg atgattaaca gcaaacagtt
    14761 gcaggcgaat attgaggtgc aaaaaatcgc cctcgcgaaa gcacaaagcg actacaaccg
    14821 ccgtgtgccg ctgggcaatg ccaacctgat tggtcgcgaa gagctgcaac acgcccgcga
    14881 cgccgttacc agtgcccagg cgcaactgga cgtcgcgatt caacaataca atgccaatca
    14941 ggcgatgatt ctggggacta aactggaaga tcagcctgcc gtgcaacagg ctgccaccga
    15001 agtacgtaac gcctggctgg cgctggagcg tactcgtatt gtcagtccga tgaccggtta
    15061 tgtctcccgc cgcgcggtac agcctggggc gcaaattagc ccaacgacgc cgctgatggc
    15121 ggtcgttcca gccaccaata tgtgggtgga tgccaacttt aaagagacgc agattgccaa
    15181 tatgcgtatc ggtcagccgg tcactatcac caccgatatt tacggcgatg atgtgaaata
    15241 caccggtaaa gtggttggtc tggatatggg cacaggtagc gcgttctcac tgcttccggc
    15301 gcaaaatgcg accggtaact ggatcaaagt cgttcagcgt ctgcctgttc gtatcgaact
    15361 ggaccagaaa cagctggagc aatatccgct gcgtatcggt ttgtccacgc tggtgagcgt
    15421 caataccact aaccgtgacg gtcaggtact ggcaaataaa gtacgttcca caccggttgc
    15481 ggtaagtacc gcgcgtgaaa tcagcctggc acctgtcaat aaactgatcg acgatatcgt
    15541 aaaagctaac gctggctaat ccagaggtgc gtgtgatgca acagcaaaaa ccgctggaag
    15601 gcgcgcaact ggtcattatg acgattgcgc tgtcactggc gacattcatg caggtgctgg
    15661 actccaccat tgctaacgtg gcgatcccca ctatcgccgg gaatctgggc tcatcgctca
    15721 gccagggaac gtgggtaatc acttctttcg gggtggcgaa tgccatctcg atcccgctta
    15781 ccggctggct ggcaaagcgc gtcggggaag tgaaactgtt cctctggtca accatcgcct
    15841 ttgctattgc gtcgtgggcg tgtggtgtct ccagcagcct gaatatgctg atcttcttcc
    15901 gcgtgattca ggggattgtc gccgggccgt tgatcccgct ttcgcaaagt ctattgctga
    15961 ataattaccc gccagccaaa cgctcaatcg cgctggcgtt gtggtcgatg acggtgattg
    16021 tcgcgccaat ttgcggccca atcctcggcg gatatatcag cgataattac cactggggct
    16081 ggatattctt catcaacgtg ccgattggcg tggcggtggt gttgatgaca ctgcaaactc
    16141 tgcgcggacg tgaaacccgc accgaacggc ggcggattga tgccgtgggg ctggcactgc
    16201 tggttattgg tatcggcagc ctgcagatta tgctcgaccg cggtaaagag ctggactggt
    16261 tttcatcaca ggaaattatc atccttaccg tggtggcggt ggtggctatc tgcttcctga
    16321 ttgtctggga gctgaccgac gataacccga tagtcgatct gtcgttgttt aagtcgcgca
    16381 acttcaccat cggctgcttg tgtatcagcc tcgcgtatat gctctacttc ggcgctattg
    16441 ttctgctgcc gcagttgttg caggaggtct acggttacac ggctacgtgg gcagggctgg
    16501 cctctgcgcc agtagggatt attccggtga tcctgtcgcc gattatcggc cgcttcgcgc
    16561 ataaactgga tatgcgccga ctggtaacct tcagctttat tatgtatgcc gtctgcttct
    16621 actggcgtgc ctataccttt gaaccgggta tggattttgg cgcatcggcc tggccgcagt
    16681 ttatccaggg gtttgcggtg gcctgcttct ttatgccgct gaccaccatt acgctgtctg
    16741 gtttgccacc ggaacgactg gcggcggcat cgagcctctc caactttacg cgtacgctgg
    16801 cggggtctat cggcacgtcg ataaccacaa ccatgtggac caaccgcgag tcgatgcacc
    16861 atgcgcagtt aaccgagtcg gtaaacccat tcaacccgaa tgcccaggcg atgtacagtc
    16921 aactggaagg gcttgggatg acacaacagc aggcctccgg ctggattgcc cagcagatca
    16981 ccaatcaggg gctgattatt tccgccaatg agatcttctg gatgtcagcc gggatattcc
    17041 tcgtcttgct ggggctggtg tggtttgcta aaccgccctt tggcgcaggt ggcggcggag
    17101 gcggtgcgca ctaagtacaa ctaagccagt tcatttgaac tggctttttt caattaattg
    17161 tgaagatagt ttactgacta aatgtgcagt tcctgcaact tctctttcgg cagcgccagt
    17221 tcttcgttgc tgttgatgcg cacgtcacgt tccagaatgc tacgcgcaat atcctgcgct
    17281 tcctgcaacg agtgcatctg gtaagtgcca cactggtaga cgttcagttc cgggatctga
    17341 ttctgatcct gcactttcag cacgtcttcc attgccgctt tccaggcatc agcaacacgc
    17401 tgctcatctg gcgtaccaat cagactcata taaaaaccgg tgcggcagcc cattggcgag
    17461 atatcgataa tctctacacc attaccgtta agatggttac gcataaaacc agcaaacagg
    17521 tgctccaggg tatggatccc tctttctggc atcacttctt tgttcggcac gcagaagcgc
    17581 agatcgaaca cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc cacccgaact
    17641 gcaggcgctt ccatccgggt atgatcgact gtgaagctat ctaacaacgg catttagcca
    17701 cctccggtaa tttttttaaa aattttctga actctttctt cccaggcgag tctgagtata
    17761 tgaaagacgc gcatttgtta tcatcatccc tgaattcaga gatgaaattt tggccactca
    17821 cgagtggcct ttttcttttc tgtcaggcgt gtttttccag ccacaccgca aacggttcgg
    17881 tatctgcggc ttccatttcc tgctgacggc gttcagacgc ctcgcgctcg gctacaaaat
    17941 cctcttcgcg cagaatttcc agcggctctt cacgcagcag attacggtag gcttctgcaa
    18001 atgctttgcc tgttccgcca ataccagtat caatcataga ccttaagata cgggcagaga
    18061 aagtcagatc gggattatcg aagcaggcga ccagttcatc acacactttc tgatacgctt
    18121 cgccgccgtt aatgctatcc agcgtttgcg cgacgcgttt cagatcgcgg aacagatctt
    18181 tacccacctg cggtaacggg aactgcgcgg tttcgcagcc gatacccagc gtcagacccg
    18241 gtttgcgacc ttcgaggatc acccggttcc agttaacgcg tgtacaggca agttcgctac
    18301 tgctcatttc cggtgcatca gccagcgcac accagaccat aaacaggtcg aggaatcgca
    18361 cctgctgttc atctacacca atcggcgaga acgggttgat gtccagcgaa cgcacttcaa
    18421 tatattcaat gccgccacgt aacagcgcat cagaaggcga ctcgccgctg cgggtaacgc
    18481 gttttggacg aatcggcgcg tacagttcgt tttcaatctg cagcacattg ctattgattt
    18541 gcagccgctt accatctttc tcaataccaa tcttcgcgaa ctcttccgat ggcgttttga
    18601 ttgcctgttt caggcccgct acgtactcgt aaagatcgtt gaaggtaata ccaagattgc
    18661 tttgcgattt attggtatag ccaagatcgc tcaaacgaag agaggtcgca tacggcaggt
    18721 aatacatacc gcactcggtt ttctcaaacg gcagcgacgt tggtttacct tgcaggaatg
    18781 aagagcatat tgccggagac gcgccaaaca gataaggaat gacccaaccg aagcgatagt
    18841 agttgcgaat aacgcggaaa tagcccgcag agattttctc tttagcatca gcgcccgaaa
    18901 tatcgccaca cttcgcttgc cagaatgcca taggcaaaga gaagttgtaa tgcacgccgg
    18961 agatggtttg catcagcgcg ccgtagcgat ttttcagccc ttcacggtac agcgttttaa
    19021 agcgtccggt gttagaagtg ccatactgcg ccagttcgat gtcctgacct tctgcgatgt
    19081 agcatggcat acttaacggc cacatccgct catcgcccat gttgcgcgcc gtatagcgat
    19141 gcaggtcgcg cataaaggtc agcatatgtt caatatcacc atccactggt gtaataaatt
    19201 ccagcaatgc ttccgcaaaa tcggtagtta tccatttatg cgtcagtgcg gaacctaatg
    19261 cttcaggatg acctgttgtt gccagtgtgc catcagcatt aacacgcaaa gtttcgcgct
    19321 ccagcccacg ctgtatcccc tttaacgcct gaggatgttt ttccagccag gccagcgcct
    19381 gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa ttagcataac
    19441 tgtaatggtg accatatgtg caggcctaca attagtgcca ccacatcatg ccctgaacgg
    19501 tcgctgctgc aactgcaaca tagcgtaacg ctttaccaag gcacaaaaaa aagattaccg
    19561 gtccccacga gatgcgcatc catcccgcta acagacacag taaatcgcca accacgggca
    19621 tccagcttaa taatagcgtg actgcaccat agcgtttcag ccagccggta gctttctctt
    19681 gccagcgcga tgttttacgc aatggaaaga aacgcccaag gataacgtta gttaaccctc
    19741 caaggctatt acccattgtt gctgttaaaa ctaaaaccca gggatgactg atcccggaaa
    19801 gcaacattgc caccagcacg acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac
    19861 tactggcaaa caacgagaaa agcgataacg cttcactcac agcaagcgaa catccacagc
    19921 gtccatgcct gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa agaccacaca
    19981 ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt
    20041 atggtgtttg acgtgatcgg cagcgacgac ggcgtcaaaa taacggcgta atcccaggtg
    20101 agccagcaat gcctcagcga tggcgctttc actccccgtt cctaccgcca ttggccgacg
    20161 tccatgccaa cttttcacca cttcaacaag aggaagcggc tcgacactat ccagcagcat
    20221 acttcttact gcttctgttt tttcacgcgc taacgcatgc gggtcgagat cggcctgatt
    20281 cagctcaata attgcctgag caatacgcca ggtgggcgat ccattaagcg caatcatcgc
    20341 ctgaacatcg tactgaagac cgtagtgccc taatacttcg cgccacgctt tacggtgcgt
    20401 aggctccgta tccaggattg tgccatccat atcaaaaatt aaacctgcat aacgctcgta
    20461 catggtcttc tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc ttgattttgt
    20521 cgctgatggc gaaggagaag aaaattcagg aaatggataa agtagttatt tagaaatgag
    20581 atatttttga aggaaatttt ttggagaaga tggtgcatcc gggaggatga ctcactggcg
    20641 ttcgcccttc gggccgttgc tggcgcaacg ttatcatccc tggtgcctgc ggttaattcc
    20701 gctacctctt cgataccttc attgctgaag atttcggaga atatggtgca tccgggagga
    20761 ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc
    20821 atcgggaaat ttattttact gctgatattg ataccgctac taaagccata tcaagtaaga
    20881 gatggtgcat tcgggaggat tactcgcctt gcggctcgcc cttcgggccg ttgctggcgc
    20941 aacgttatcc tccctggtgc ctgcggttaa ttccgctacc tcttcgatat cttcattgct
    21001 gaagatttcg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    21061 agccgagtac tctatccagc tgagctacgg atgcatcggg aaacttattt tactgctgat
    21121 attgataccg ctactaaagc catatcaagt aagagatggt gcatccggga ggattcgaac
    21181 ctccgaccgc tcggttcgta gccgagtact ctatccagct gagctacgga tgcatcggga
    21241 aacttacttt actgcagatt ttttgatacc gctactaaag ccgtatcaag taagagatgg
    21301 tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc
    21361 tgagctacgg atgcaaatgg cggtgaggcg gggattcgaa ccccggatgc agcttttgac
    21421 cgcatactcc cttagcaggg gagcgccttc agcctctcgg ccacctcacc acacgcctct
    21481 tacgagtgct tcgaagaact tgtttatcgc tcatcgtcgc tgcgtggcgc acatattact
    21541 ttctgagact tataagtcaa acaatttttc ccacactttt atcgtttgca cacttcacgt
    21601 tcaattagtc tgtaaaaacg tcaaaaagag tgttttatca acagaaaaat ggaggtctga
    21661 cagatagcag taatgtaaaa aaatggagac ttaagttgaa tgaacgggag taaagcgaaa
    21721 agactataga gtgaaggaga aattttgagg gtgcgtctca ccgataaaga tgagacgcgg
    21781 aaagattagt aactggactg ctgggatttt tcagcctgga tacgctggta gatctcttca
    21841 cggtgaacag aaacttcctt cggggcattt acgccaatac gtacctggtt gccctttacc
    21901 cctaaaactg tcacggtgac ctcatcccca atcatgaggg tctcaccaac tcgacgagtc
    21961 agaatcagca ttctttgctc cttgaaagat taaaagagtc gggtctctct gtatcccggc
    22021 attatccatc atataacgcc aaaaagtaag caatgacaaa cacattacat ctaagcagtc
    22081 atggcattac attctgttaa acctaagttt agccgatata cacaacttca acctgacttt
    22141 atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag tccacggcat tgccttacgc
    22201 ttatattatt gcaatttcgc gctgacccag cctttcacac tggctaacgc tgcaggtaag
    22261 gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac gtccaccacc cttgccgccc
    22321 acctgctgag cgaccatacc aatcagttcc cctgctttca cacgatctgt gacgtcctta
    22381 gatacgcctg caatcagaga aaccttacct tcggctaccg ttgccagcac gataattgtc
    22441 gaccccagct gattttttaa atcgtcaacc atggtacgca acattttcgg ctcaacaccg
    22501 ctaagctcgc taaccaacag cttaacacca ttaacatcaa ttgccttact ggaaagattt
    22561 gcgctctcct gtgcggcagc ttgttcttta agctgttgta gttctttttc taactgacgc
    22621 gtacgttcca gtactgagcg cactttatca gccagattat tgctatcgcc tttcagcaga
    22681 tgtgcgactt cgcttaagcg atcgctgtct gcatgaacgg tggcgatagc gccttctccg
    22741 gttaccgctt caatacgacg aacgcctgca gcagtacccg attcagagat gatgcggaac
    22801 agaccaatat caccagtgcg gctggcgtga gtaccgccac acagctcggt ggagaaatcg
    22861 cccatgctca gcacgcgtac gcgctcatca tacttctcgc cgaacagcgc catcgcacct
    22921 ttcgctttcg ccgcttcgag atccatgatg ttggtttcga tcggcaaatt gcgacgaatc
    22981 tgtgcgttca ccaggtcttc gaccgcacga atctcttccg gtttcatcgc ttcgttgtgt
    23041 gagaagtcga agcgcagcac cttgtcgtta accagtgaac ctttctgcga tacatgagta
    23101 cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg cggagtgatt cagacgaata
    23161 cgggcgcgac gagcctcatc aacatcagcc tgcacagcgt cgcccacttt cagagaaccc
    23221 gcagcaagtt taccgatgtg accaatcgcc tggccgtatt tctgcgtatc ttccaccacg
    23281 aaggagaagt tagcgccttt cagttcgcct ttatcaccaa cctgaccgcc ggattccgca
    23341 tagaatggcg tttgatccag cacgaccaca gcttcctggc ctgcattgat ggcatcaacc
    23401 gctttaccat caacaaacag cgcggtcact ttgccgttca gttccagatg gtcatagcct
    23461 ttaaattcag atgcactgtc aacacggatc attgcgttgt aatcggcacc aaagccgctg
    23521 gcttcgcgcg cccgacgacg ctgctcttcc attgctgctt caaaaccagc ttcgtcaact
    23581 ttgatgttgc gctcacgaca aacatcagcc gtcaggtcaa ccgggaagcc ataggtgtcg
    23641 tacagacgga aagcagtttc accatccagc gtatcaccag aaagttttgc cagctcttca
    23701 tccagcaacg ccagaccgcg ctccagagta cgagcaaact gctcttcttc agtcttcagc
    23761 acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac ccgcagagcc cataacgtcg
    23821 atcagcggac caaccagttt gtagaagaag gtttctttcg cgccgagcat attaccgtga
    23881 cgcactgcgc gacgaatgat acgacgcagt acataaccac ggttttcatt ggacggcatt
    23941 acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt cagcgattac gcgcagcgat
    24001 ttattgctca gatcggttgc gccagtgact ttcgctaccg cctggatcaa cgtgcggaac
    24061 aggtcgatgt catagttaga gttaacgtgt tgcagcaccg cagcaatacg ctccagaccc
    24121 ataccggtat ctacagacgg cttcggcagc ggttccatcg tgccatcggc ctggcggttg
    24181 aactgcatga agacgatgtt ccagatctca atgtagcggt cgccgtcttc ttccggactt
    24241 cccggagggc caccccaaat gtggtcgccg tgatcgtaga agatttcggt gcacgggccg
    24301 cacggaccag tgtcacccat ctgccagaag ttatcagatg cgtatggcgc acctttgtta
    24361 tcgccgatgc gaataatacg ttcgcgcggg atccctactt ctttttccca gatttcgtag
    24421 gcttcgtcgt cgctttcata gacggtaacc cacagacgct ctttcggcag ggcaaaccat
    24481 ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat cgtgtttgaa atagtcgccg
    24541 aagctgaagt tgcccagcat ttcgaagaag gtatggtgac gcgcggtgta accgacgttt
    24601 tccaggtcgt tgtgtttacc accggcacgc acgcagcgtt gggaagtggt agcgcgggaa
    24661 taattacgct tgtcgagccc aaggaacaca tccttgaact ggttcatccc ggcgttggta
    24721 aacaacaaag ttgggtcgtt atggggtacc agggagctgc tggcaactac ctgatgtccc
    24781 ttactatgga aaaagtcgag aaacgcctga cggatctcag cggtgctctt gctcataatt
    24841 atcctgaaat caagctaacg aaatatcgcc accagctcca gcgtgtctta accgccgggc
    24901 tggtaactga aaagtgggaa taagataagt tttcttgact gggaagtaaa ataccgtatg
    24961 cgttcagtcg gcaaaatttc gccaaatatc ctggatatct tccatcagat agccacgata
    25021 gagcagaaaa cgctggatct taactttttc tgaaaagaca gttggcaaag gttcaccata
    25081 ttttcgcgtc gcctgatcgc gcgccagtgc gcaccagtcg atgtcacatt cacgcatcgc
    25141 tttttctgtc gcttcgcggg aaataccttt ctgattcagt tcctggcgaa tacgcgcagg
    25201 tccataacct ttgcggctac ggctggcgat aaagcgcgca acaaatcggc tgtcatcgag
    25261 atagccatgt tcatggcacc aggcaataac gcgctcgtaa tcttctgccg tagcatcaat
    25321 ctcttctggg ccatttttgc ccataatcgg tgccgcgagt ttacgtcgca gttcttgctc
    25381 actgtgatcg cgcaccgcca gaatgcgtac cgcacgatcc aacaggcgag catatgccgg
    25441 gcgacgggac gttgattctg tcatggcata tccttacaac ttaaaaaagc aaaagggccg
    25501 cagatgcgac ccaggttttg ctgataaaca gatttaatag tcaatgagtg tgcagaatgt
    25561 tcccgcaggc ccaattcgtt agaggatcga ggtgagagta atttttcacg ccagtgctgg
    25621 cctgttcccc tcaccctaac cctctcccca aaggggcgag gggaccgtcc gtgcacacat
    25681 tatctattat catcaataac gaacgattaa aaatcttcgt tagtttctgc tacgccttcg
    25741 ctatcatcta cagagaaatc cggcgttgag ttcgggttgc tcagcagcaa ctcacgtact
    25801 ttcttctcga tctctttcgc ggtttccggg ttatctttca gccaggcagt tgcattcgct
    25861 ttaccctgac cgatcttctc acctttgtag ctgtaccacg cgcctgcttt ctcgatcagc
    25921 ttctctttta cgcccaggtc aaccagttcg ccgtagaagt tgataccttc gccgtagagg
    25981 atctggaatt cagcctgttt aaacggcgca gcgattttgt tcttcaccac tttcacgcgg
    26041 gtttcgctac ccaccacgtt ttcgccctct ttcaccgcgc cgatacgacg gatgtcgaga
    26101 cgaacagagg cgtagaattt cagcgcgtta ccaccggtag tggtttccgg gttaccgaac
    26161 atcacaccaa ttttcatacg gatctggttg atgaagatca gcagcgtgtt ggactgcttc
    26221 aggttacccg ccagcttacg catcgcctgg ctcatcatac gtgccgcaag gcccatgtga
    26281 gagtcgccga tttcgccttc gatttccgct ttcggcgtca gtgccgccac ggagtcaacg
    26341 acgataacgt ctactgcgcc agaacgcgcc agggcgtcac agatttccag tgcctgctcg
    26401 ccggtgtccg gctgggagca cagcaggttg tcgatatcga cgcccagttt acgtgcgtag
    26461 attgggtcca gcgcgtgttc agcatcgata aacgcacagg ttttaccttc acgctgcgct
    26521 gcggcgatca cctgcaacgt cagcgtggtt ttaccggaag attccggtcc gtagatttcg
    26581 acgatacggc ccatcggcag accacctgcc ccaagcgcga tatccagtga aagcgaaccg
    26641 gtagagatgg tttccacatc catggaacgg tcttcaccca ggcgcatgat ggagccttta
    26701 ccaaattgtt tctcaatctg gcccagtgct gccgccaacg ctttctgttt gttttcgtcg
    26761 atagccattt ttactcctgt catgccgggt aataccggat agtcaatatg ttctgttgaa
    26821 gcaattatac tgtatgctca tacagtatca agtgttttgt agaaattgtt gccacaaggt
    26881 ctgcaatgca tacgcagtag cctgacggcg caccgcatca cggtcgccgc tgaagcattc
    26941 ccgccgggta atgccttcac cgcgggcagt ggcaaaagca aaccagacgg tgccgacagg
    27001 cttctcttca ctgccgccat ccggcccggc gataccacta atagacacgg cgtaatcagc
    27061 acgagccgct ttcagtgcgc ctatcgccat ttccaccacg acgggttcgc tcaccgcgcc
    27121 atgctgcgcc agcgtctctt cgcgtacgcc gatcatttgc gctttcgctt cgttactgta
    27181 ggtgacaaat ccgcgttcaa accaggcgga actgccggca atatcggtaa tcactttcgc
    27241 tacccaaccg ccggtacaag actcagcagt tgttacggtt gcgccacggg ctttcagcgc
    27301 ctgcccaacc tgttcgctta actgcatcag ttcactgtca gtcatcacaa cctctgtcag
    27361 tcaaaaattt atgccggaca agatagcact ttcgtcagga agatggtgtt ggcgttgcaa
    27421 ttttacgagg aggattcaga aaaaagctga ttagccagag ggaagctcac gcccccctct
    27481 tgcaaatagt tactgcacgc gcgccagcgc cacggcttgt cctaactgcc agaccgccat
    27541 tgcgtaatgg gtgctgtggt tataacgggt aatggtgtag aagttcggca taccgtacca
    27601 gtactggtaa ccggtaccga catccagacg cagcaggctg gcttgttgat ggttgcccag
    27661 cggctgctgt ggcgttaaac ctgccgcggc aagctgcgaa atgctgtact tggttttgaa
    27721 gccatttggc aagcctggag cctgaccgtt tgccattacc gcgacctgat cgcctttcac
    27781 ccagccgtgc gctttgaaat aattcgccac gctaccgatc gcatcaaccg gatcccacag
    27841 gttgatatgc ccatcgccgc tgaaatccac tgcatattgt ttgtaagacg acggcataaa
    27901 ctgaccatag cccatcgccc cggcaaagga acctttcaga ttgagcggat cgtcctgctc
    27961 gtcgcgcgcc atcaacagga aggtttccag ctcgccagag aaatactccg cgcggcgtgg
    28021 gtagttaaat gacagcgttg ccagcgcatc gaggatgcga gttttcccca tcacgcgccc
    28081 ccagcgagtt tcaacgccga taatcccgac gataatttcc ggcggtacgc catacacctg
    28141 ccacgcgcga ttcaacgcat cttcatactg attccagaaa accacaccgt tctgcacgtt
    28201 gtccggcgta ataaattttt tgcgataacg cagccatgcg ccgttcggac ctgatggcgg
    28261 tttcaccgat gtggttggtg cctggttatc catcagccgc agtaccgaat ccagacgctt
    28321 cgcctgggag agaatttcct gcaactgctg acgatcgaaa ccgtgtttgt tcaccattct
    28381 gtcgatgaac tgctgggcat tcgggttatt agcgaaatcg ccgcccatct gcatcacatt
    28441 gtgctgcggc tcaagcagga agccgccaga cggcgttccg gtggtcgttt cagtctcagt
    28501 aggttttggc ttgctgctac aggcggcaag caacacaaaa aggggaagca atgttacata
    28561 acgacgcttg aacatgaggg gtccatttaa cagattcaac caggggcaag tatggtaaag
    28621 catcacgccc cgcacaagga agcggtagtc actgcccgat acggacttta cataactcaa
    28681 ctcattctcc tcgctatcct tttactcaaa ctttcaaatt aaaatattta tctttcattt
    28741 tgcgatcaaa ataacacttt taaacctttc aatctgatta gattaggttg ccgtttggta
    28801 ataaaacaat aaatcctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag
    28861 tggtttatcg ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt
    28921 cttccgctgt tgattagtct gctggttatc atgaacgcac tgattaattt tatcggtcag
    28981 catcgtattg aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctgctg
    29041 ttaccgtgca ttggcacgtt tgtcttttgc aatccgatga ccttaagcct gggtcgcttt
    29101 atgccggaaa agtacaaacc cagctactac gcggccgcct cttatagctg ccactcaatg
    29161 aatggcctct tcccccatat caacccaggc gaactgtttg tttatcttgg cattgccagc
    29221 ggtctgacaa cgctgaacct gccacttggc ccactggcgg tgagctatct gctggttggt
    29281 ctggtcacca acttcttccg cggctgggtg accgatctga ccaccgccat ttttgagaaa
    29341 aagatgggta ttcaacttga acaaaaagtt catctggcag gagcaacatc atgacgcgta
    29401 ttcggatcga aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg
    29461 gcaaaaaaat cgtctatatc accgccggta cccggcctgc gattgttgac aaactggtac
    29521 agcttactgg ctggcaggct atcgacggat ttaaagaagg tgaacccgcg gaggcggaaa
    29581 ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg cggcatctat ccgaaacggc
    29641 gtattcccac cattaatatc cactcgacgg gcaagtccgg tccactggcg cagtacattg
    29701 tggaagatat ttatgtctca ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga
    29761 caccacaacc ctcttccgtg ggccgtgact atgacaccag caagaaaatc actgaacaaa
    29821 gcgatggttt actggcgaag gtgggaatgg gtatgggttc tgccgttgcc gtgttgtttc
    29881 aatctggtcg tgacaccatc gacactgtat taaaaaccat tctgcccttt atggcattcg
    29941 tttcggcgct cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc
    30001 ttgctccgct ggcgagccat ccactgggtc tggtgatgct ggcgctcatc tgctccttcc
    30061 cactgctttc acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga
    30121 ttggcgtgca gattggtctc agcaatattc cgccgcatct ggctttacct gcgctgtttg
    30181 ccatcaacgc gcaggcggcc tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc
    30241 gtcaggacac ggttcgcgtc ggtgtccctt ctgttctggt gagccgcttt ttaaccggcg
    30301 caccgactgt actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac
    30361 atgaccgtta tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc
    30421 agcgaccaga tgctcatcac ctttcgtgaa ggcgcgccag cggacctcga agagtattgc
    30481 ttcattcatt gccacggcga gttgaaaggt gcactccatc ccggtttgca attttcgctc
    30541 gggcagcatc gctatccggt gaccgccgtt ggcagcgtgg cggaagacaa ccttcgcgaa
    30601 ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc
    30661 catgtggcag gccctgtccc cgacgatatc gcgccgggat cggttttgaa gtttgaatct
    30721 gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc
    30781 gcgttcctgt gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag
    30841 agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aggaacagcg
    30901 tatggttttg gtgctgacgc caccagcgag caaagcgttc tggcactctc tcgtggggta
    30961 gatgaaatct ttggtcgcgt ggatttgctg gtctacagcg ccggaattgc aaaagcagcc
    31021 tttatcagcg acttccagct cggcgatttt gaccgttcgc tacaggtgaa tctggtgggt
    31081 tatttcctgt gtgcccgtga attttcgcgt ttaatgatcc gcgacgggat tcaggggcgc
    31141 attattcaga tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc
    31201 gccgcgaaat ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggaatac
    31261 ggcattacag tgcattcact gatgctaggt aacctgctga aatcgccgat gttccagtct
    31321 ttgctaccgc aatatgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac
    31381 atcgacaaag taccgctcaa acgcggctgc gattatcagg atgtgctgaa tatgcttctg
    31441 ttctacgcca gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt
    31501 caggtgatgt tctgatcaac agcggagatc cgttaaggat ctccgtgaga ctaataggat
    31561 gcctgatgcg ctacgcgcat caggcataca ggacttccgc cactacatta aggaaaagtt
    31621 atggtatccg cactcatcac cgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc
    31681 ggctggcaaa tttctcgttt taaccgtgcc ttcgacacac tatgccagca agggagggtt
    31741 ggcgtgggcc gttccagcgg acgctttaaa ccgcgggtcg tggtcgccat cgccctggac
    31801 gatcagcagc gcatcgtcga caccttgttt atgaaaggac tgaccgtttt cgccaaaccg
    31861 caaaaaatac ccgcaattac gggtatgtat cctggtgatt tacggcccga tgtgatcttt
    31921 ccccatgatc cactatcaca gaatgctcta tcattggcgc ttaaactgaa acgtggataa
    31981 tttcgttgtg aatgttactt gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc
    32041 acgcccatga aacctcgtca gcgtcaggcc gccattctgg agtatctgca aaagcagggt
    32101 aaatgctcgg ttgaagaatt ggcgcaatac tttgacacca caggcacaac cattcgcaaa
    32161 gatctggtca ttctggaaca tgccggaacc gtcattcgta cttatggcgg agtgatgttg
    32221 aataaagagg aatccgatcc gcctatcgat cataaaacac tcatcaacac ccacaagaaa
    32281 gagctgattg cagaagctgc cgttagtttt atccatgatg gcgattcgat cattcttgat
    32341 gctggcagta ccgttttgca gatggttccc ctgctctcgc gctttaataa catcacggtg
    32401 atgaccaaca gcctgcatat cgtcaatgcg ctatccgaac tggataacga acaaactatc
    32461 ctgatgccag gcggaacgtt tcgtaaaaaa tcggcctcat ttcacgggca gctggcagag
    32521 aatgccttcg agcatttcac cttcgacaaa ttattcatgg gtactgacgg catcgatctc
    32581 aatgcgggcg taaccacctt caacgaggtt tataccgtca gtaaggcaat gtgcaatgcc
    32641 gcgcgcgaag tgattttgat ggcggactca tcaaagtttg gccgtaaaag ccccaacgta
    32701 gtttgcagtc ttgaaagcgt cgataagctg attaccgacg cagatatcga tccggcgttt
    32761 cgtcaggcgc tggaagagaa agggatcgat gtgatcataa ccggagagag caatgagtga
    32821 agcactactg aacgcgggac gtcagacgtt aatgctggaa ttgcaggaag caagccgttt
    32881 accggaacgt ctgggcaatg attttgttcg cgccgccaat atcatcctgc actgcgaagg
    32941 caaagtggtg gtttcgggaa ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac
    33001 gcttgccagt actggcacac cggctttttt tgtccatccg gcagaagcac tacacggcga
    33061 tctggggatg atcgaaagcc gcgatgtgat gctgtttatt tcttactccg gtggcgcgaa
    33121 ggaactggat ctgattattc cgcgtctgga agataaatct atcgcgctgc tggcgatgac
    33181 cggcaaaccg acgtcaccgc tgggcctggc ggcgaaagcg gtgctggata tctccgtaga
    33241 acgcgaagcc tgcccgatgc accttgcacc gacctccagc accgtcaata ccctgatgat
    33301 gggtgacgcg ctggcgatgg cggtcatgca ggcgcgcgga tttaatgaag aagattttgc
    33361 ccgctcccac ccagccgggg cactgggcgc tcgcttgctg aataaagtgc atcatctgat
    33421 gcgtcgtgat gacgctatcc cacaggtagc gttaaccgcc agcgtgatgg atgcgatgct
    33481 ggaactcagc cgcaccggtc tggggctggt ggcggtatgt gacgctcaac aacaggtaca
    33541 aggcgtcttt accgacggcg atttacgtcg ctggctggtt ggcggcggcg cactcaccac
    33601 gccagttaat gaagcgatga cgacaggcgg taccacatta caggcacaaa gccgcgccat
    33661 cgacgccaaa gagatcctga tgaagcgcaa aatcactgcc gcaccggtgg tagatgaaaa
    33721 cggcaaactc gtcggcgcaa ttaacctgca ggatttctat caggccggga ttatttaatc
    33781 cttcaatccc agacgtttcg ccagccgatg caggttggcg acgtcggttt ccagcattcg
    33841 cgcgcaggct gcccagttgt gatgattttg tgccagtgcc tgacgaatag tttcacgctg
    33901 gaacgcttct gtcgcttcac gcaggttttg cttaacaacg ggcaccgccg ccgcttctgg
    33961 cggcggtaac gtcacctcag gaaaagcaaa atgttgcgcc tcaagaatca cttcatcgcc
    34021 gttgcgggtg gctctcgcca gcactaccgc ccgatgaata gcatgttcca gttcgcgcac
    34081 gttccccgga aaacgatagt gttgcagtaa atttcgcgct ccggcactta ataccacgcg
    34141 ggagagcccc agccgcaaac gacactgctc gcagaaatac cccgccagca gaatgacatc
    34201 atcgccccgc tcacgcagcg gcggcaccga aagtggaaac acgctcaggc ggtggaacaa
    34261 gtcagcgcga aatcgccctg ccagcacctc ttcgcgtaag tcgcggttag tcgccgccag
    34321 cacgcgcaca tcgacccgca aactgcggtc atcgccaacg cgctgaatat cgccatactg
    34381 caacacccgc agcagcttgg cctgcaatgc caacgacaac tcgccgatct catcgagaaa
    34441 cagcgtgccg ttatccgcca tttcaaactt cccactgcgg ttactgatag cgccagtaaa
    34501 cgctcctttc acatgcccga acaactcact ttccgccaca ctttccggca gtgcggcaca
    34561 gttgagatag accagcggat tcaccgcccg tggcgaggcc tcatgaatcg ctttcgccac
    34621 cagctcctta ccggttcccg tctcaccgct gatcaggacg ttgagatcgg acgccgccac
    34681 aatctcaatc tcttttttca gttgcgtcat gcctggcgac agaccgatca tctgcgtctg
    34741 tttcaccgct tcaaatggag cggcatcgcc tggcagcata ttctggcttt ccagttgttc
    34801 aatcagcaac gcattgctta acgctcccgc cgccagcgcg gcaatcaggc gtaactcttc
    34861 gtcgctgaaa acatcgaact gatcgggctg catcccgtcg agcgtcaatg cgccgatcag
    34921 gttttgcccg gcaaacagcg gcagaccaac gcaggcgtga accttcagac tctcctgccc
    34981 tggaatcaaa ccgtcatagg gatcgggcaa ttcgctgtct gcgggaaagc gcaccacatc
    35041 acccgcgcgg gcaatcgctt ccagccgtgg atgaccttcc agcgcaaagc gtctaccgag
    35101 tacatccttc gccagcccgt cgatggcaag cggaataaac tgccgcgagt cgtaacgtag
    35161 caacgcagac gcatcgcact ccagcacctg acgcagcgtg gtgatcaggc gctgaaaacg
    35221 atcctgatga ccaatcccac gctgcaattc gatggcgata ttcgccagca catcaacgga
    35281 aaaactcatc tttgcctcac tgtcaatttg actatagata ttgtcatatc gaccatttga
    35341 ttgatagtca ttttgactac tcactaatga gcataatttt atttatagag taaaaacaat
    35401 tagataaaaa actggcacgc aatctgcaat tagcaagaca tctttttaga acacgctgaa
    35461 taaattgagg ttgctatgtc tattgtggtg aaaaataaca ttcattgggt gggtcaacgt
    35521 gactgggaag tgcgtgattt tcacggcacg gaatataaaa cgctgcgcgg cagcagctac
    35581 aacagctacc tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga ccataaattc
    35641 agccgcgaat ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat cgattacatc
    35701 gtgattaacc atgcagaaga ggaccacgcc ggggcgctga ccgaactgat ggcacaaatt
    35761 cccgatacgc cgatctactg tacagccaac gctatcgact cgataaatgg tcatcaccat
    35821 catccggagt ggaattttaa tgtggtgaaa actggcgaca cgctggatat cggcaacggc
    35881 aaacagctca tttttgtcga aacaccaatg ctgcactggc cggacagcat gatgacttac
    35941 ctgacaggcg acgcggtgct gttcagtaac gatgctttcg gtcagcacta ctgcgacgag
    36001 catctgttca acgatgaagt ggatcagacg gagctttttg agcagtgcca gcgttactac
    36061 gccaatatcc tgacgccgtt cagccgcctg gtaacgccga aaattaccga gatcctgggc
    36121 tttaacttac cagtcgatat gatagcgacc tctcacggcg tggtatggcg cgataacccg
    36181 acgcaaattg tcgagctgta cctgaaatgg gcggctgatt atcaggaaga cagaatcacc
    36241 attttctacg acaccatgtc gaataacacc cgcatgatgg ctgacgctat cgcccagggg
    36301 attgcggaaa ccgacccacg cgtggcggtg aaaattttca acgtcgcccg aagcgataaa
    36361 aacgaaatcc tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg
    36421 aataacgtga tgatgccgaa aatcgccggg ctggtggagg agatgaccgg attacgcttc
    36481 cgtaacaaac gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt
    36541 ctttccacgc gcctgcagga tgcgggtttc gaaatgtcgc ttagcctgaa agcgaaatgg
    36601 cgaccagacc aggacgctct ggagttatgc cgtgaacacg gtcgcgaaat cgcccgtcag
    36661 tgggcgctcg cgccgctgcc gcagagcacg gtgaatacgg tagttaaaga agaaacctct
    36721 gccaccacga cggctgacct tggcccacgg atgcagtgca gcgtctgcca gtggatttac
    36781 gatccggcaa aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtc
    36841 ccggataact tcctctgccc ggaatgctcc ctcggcaaag acgtctttga agaactggca
    36901 tcggaggcaa aatgagtaac ggcattgtga tcattggttc gggcttcgcc gcccgccaac
    36961 tggtgaaaaa tattcgcaaa caggacgcca gtattccatt aaccctgatt gccgccgaca
    37021 gcatggatga gtacaacaaa cctgacctca gccatgttat cagtcagggg caacgtgccg
    37081 atgaccttac ccgccagacg gcgggtgaat ttgccgagca gtttaatctg cacctgtttc
    37141 cgcacacctg ggtgacggat atcgatgccg aagcccgtgt ggtgaaaagc cagaataatc
    37201 agtggcaata cgacaagcta gtactggcaa ccggtgccag tgcctttgtc ccgcctgtgc
    37261 ctgggcgtga gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc
    37321 aactgcggga tgcccgacgc gtgttgattg ttggcggtgg tttgattggt agcgaactgg
    37381 cgatggattt ttgtcgtgca ggcaaaatgg ttacgctaat cgacaacgct gccagtattc
    37441 tggcgtcgtt aatgccaccg gaagtaagca gtcgcttgca gcatcggttg acggagatgg
    37501 gcgttcatct gctgttgaaa tctcagttgc aggggctgga aaaaacggat tctggcattc
    37561 aggcaacgct ggaccgccag cgcagtatcg aagtggatgc ggtaattgcc gccaccggac
    37621 tgcgcccgga aaccgccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg
    37681 tcgatagtta tctgcaaacc agtaatgccg atatttacgc gctgggcgat tgcgcggaaa
    37741 ttaacggtca ggtattgccg ttcctgcagc cgattcaact tagcgcgatg gtgctggcaa
    37801 aaaatcttct cggcaataac acgccgctga aactaccggc gatgctggtg aaaatcaaaa
    37861 cgccggaatt accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaga
    37921 ttaataccga acgccaggga atggtggcgc gcggcgttga cgatgctgac cagcttcgcg
    37981 cctttgtggt cagtgaggat cggatgaaag aggcatttgg attgttgaaa acgttgccgg
    38041 tgtaggtggg ctactgtgcc taaaatgtcg gatgcgacgc tggcgcgtct tatccgacct
    38101 acggggagcg catgtgttgg tcacataatg cgttcatgtc acatacagct actgtgccca
    38161 aaatgtcgga tgcgacactg gcgcgtctta tccgacctac ggggtgcgca tgtgtaggcc
    38221 ggataaggcg tttatgccgc atccggcaat aatggtgttc agatgcaaca cgttttatcc
    38281 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    38341 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    38401 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    38461 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    38521 atgaaacgcc cacgcgcgtt gattaaccgg tgcctgccag ttcagccact gctgccagaa
    38581 agtggcgaga tccagttgat tgtccaccag cggcattgtc accggatgcg tcactccgtg
    38641 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    38701 tggcgcacag cccagtgccg ccgccactgc atcgaacacc cgcccacacg atgacgccag
    38761 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    38821 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    38881 gttacgccac ggctgcttcg ccgccaaatc gccccccgga agcgccactg caggcaagcc
    38941 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    39001 gttctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc cctccagtgg
    39061 ccactgatgc tctgccagac acgccgccgc gtgggcatga tgatgcagca ccgtttgcgt
    39121 cggcagattc atttcgcgcg cccactggct ggagacatag ccaggatgtg cgtcatgcac
    39181 aacgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    39241 catctggatg ccatcgtcac ttaaatcgcc cagatgctgg ctcaacaccg cttgttcacc
    39301 gcgcaccagg cagaaggtat ttttcagatc cgcgccgaga cacagcacag gcggaacatt
    39361 tttaaagccc ggaggcaaag ccagcgcatc cggcacatac ccccgcgaac ggcgcatcat
    39421 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtat
    39481 taagaatccg tcagcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagccgg
    39541 tggtttaccg ctcaggttgc cagaggtcat caccagcggg cattgcagtt cctgtaacaa
    39601 caaatgctgg agcgggttcg caggcaacat taccccgact tcgttaaggt caggggcgat
    39661 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cggcgggcgt
    39721 ggtaagcaac tggcgcgcag cgtctggtaa accgtcagcc actggcaaca tgaccgccag
    39781 cggtttcgcc gggcgatgtt tgcgcgcccg aagtgtcgcc accgcgttac tgttacgtgc
    39841 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    39901 ctgtgcgata gccgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    39961 ttcaagatgc ggaccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    40021 cgggtcgcgg tattctttat cacaggccgg acacagcgga aacgccgcca tcacggtaaa
    40081 cgggcgatcg taaggcatgg cgcgaataat ggtgaaacgc ggaccacagt gagtacagtt
    40141 gataaacgga taacgataac gccgttcgcc tggggtattc atttcggcaa gacaggcagg
    40201 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgcctgtgc tctggcggat
    40261 agtgaactca gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    40321 cgccagcggc gggcagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcaa
    40381 ccggacttcc acgccatcgc cgtcattaca gacatcgccg tgaagattta attgctgtgc
    40441 cagttgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    40501 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    40561 cgcccagccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    40621 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    40681 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattgcga
    40741 tcgacacaaa ttagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    40801 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagccctgcg
    40861 ccgctgttgc gaatcaccgg acgtaccacc acttccatcg cgccatacgg gcaagccaca
    40921 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacat gaacaaaccc tttatcacgg
    40981 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    41041 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    41101 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    41161 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    41221 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt cactgttatt
    41281 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    41341 atgtgatgac aatgtttatc gcgaaagagc aatgagtgag tcgcggcaga tcagttttcc
    41401 gctgaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    41461 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    41521 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    41581 gcgctcgtgt aacgctttca tcgcacctat cgccatatcg tcgttactgg caactaacgc
    41641 gctaaattta gccccacgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    41701 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg ccagcgcgtc
    41761 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    41821 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaaactgg tctgtttatg
    41881 atcgcaccag acgctatggc tgctgttttt gcgcaggcgg cgattaagca ccattatcgg
    41941 ctgactgtgc gcgtcaatga tgtcatcgat ctcatccacg cttaaaaaac gcgggtaaat
    42001 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    42061 gctgtgttta ccatctgcca atagcaactg ccgccctttc tcttccgcca ttcgcgcggc
    42121 atgaaagagt aattcactaa aataaatgcc gtggtaaagc gtgttggtca ccaccagccc
    42181 cagcgtctga gtactcttcg ccgacagatt gcgcgccagc aagtttgggc ggtaaccgct
    42241 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    42301 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    42361 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    42421 gtccttgcgc aatcggcggg aaaaatattc aggtgaccgg tttcacaaat ataaaaaatg
    42481 aacaattaac tctcttgctt atttggtgac aactattcat gattttgtga aaccggtttc
    42541 ttaattccgt ttcagcatcg gcatttttcc gtcacgtcga ctgataacaa ctacatctac
    42601 cctactgata acaggataaa atccgatggc caaaaattat gcggcgctgg cacgctcggt
    42661 gatagcggca ctgggcggcg ttgataacat ctcggcagtc acgcactgta tgacgcgttt
    42721 gcgctttgtt atcaaagatg atgcgcttat cgacagcccg acgttaaaaa ccatccccgg
    42781 cgtgctcggc gtggtacgca gtgacaacca gtgtcaggtg attatcggca ataccgtttc
    42841 acaagccttt caggaaatcg tcagcctgct gccgggagat atgcaccccg ccctgcccgt
    42901 gggtaaaccc aaactcacgc tgcgtcgcat tggtgcgggg atcctcgatg cgctgatcgg
    42961 caccatgtca ccgctgatcc cggcgattat cggcggatcg atggtcaaac tgctggcgat
    43021 gatcctcgag atgagcggcg tgctgacaaa aggatcgccg accttaacca ttctgaatgt
    43081 gattggtgac ggtgctttct tcttcctgcc gctaatggtc gcggcttcgg ccgccatcaa
    43141 atttaaaacc aatatgtcgc tggcgattgc catcgcgggt gtgctggtac atccgagttt
    43201 tattgaatta atggcgaaag cggcccaggg tgaacatgtc gaatttgccc tgatcccggt
    43261 caccgcggtg aaatacacct acacggtgat cccggcgctg gtgatgacct ggtgcctgtc
    43321 atatatcgaa cgctgggtgg acagcattac accggcggtg accaaaaact tcctcaagcc
    43381 aatgctgatt gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc cgattggtat
    43441 ctggatcggt agcgccattt cggcgctggt ttacaccatt catggttatc tgggctggct
    43501 ttcagtcgcc attatgggcg cgctgtggcc tctgctggta atgaccggga tgcaccgcgt
    43561 ctttacgcca accatcattc agaccattgc cgaaaccggc aaagaaggga tggtcatgcc
    43621 gtcagagatc ggcgctaacc tgtcgctggg cggttcatca ctggcggtgg cgtggaaaac
    43681 gaaaaacccg gaactgcgcc agactgcgct ggctgcggcg gcatcagcca ttatggcggg
    43741 gatttccgaa ccggcgttat acggcgtggc gatccgcctg aaacgtccgc ttatcgccag
    43801 tcttatcagc ggttttattt gcggcgcggt tgccggtatg gcggggcttg ccagccactc
    43861 aatggcagcg ccggggctat ttaccagcgt gcagttcttc gatccggcga atccaatgag
    43921 catcgtctgg gtgttcgcgg tcatggcgct ggcggtcgtg ctgtcgttta tcctcacact
    43981 gttgctcggc tttgaggata ttcctgttga ggaagcggct gccgaggcgc gaaagcatca
    44041 gagcgtacaa ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa aatgtcagta
    44101 tttccagaag gttttttatg gggcggcgcg cttgccgcca accagtctga aggtgcgttc
    44161 cgtgaaggtg gcaaaggact gacaacggtc gatatgatcc cacacggcga gcatcgaatg
    44221 gcggtgaaac tggggctgga aaaacgtttt cagttgcgcg atgacgagtt ttatcccagc
    44281 catgaggcga cggattttta tcatcgttat aaagaagata tcgccctgat ggcagagatg
    44341 ggattcaagg ttttccgtac ctcaattgcc tggagccgtc tctttccgca gggcgatgaa
    44401 atcacgccca atcagcaggg cattgctttt tatcgttctg tctttgaaga atgtaaaaag
    44461 tacggtatcg aaccgctggt cacgttgtgc cacttcgatg tgccgatgca tctggtcacc
    44521 gaatatggct cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta cgcccggacc
    44581 tgctttgaag catttgatgg tctggtgaaa tactggctaa ccttcaatga aatcaacatt
    44641 atgttgcata gcccgttctc cggcgcgggt ctggtgtttg aagaaggtga aaatcaggat
    44701 caggtgaaat atcaggccgc gcatcaccag ctggttgcca gtgcgctagc caccaaaatc
    44761 gcccatgagg ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg caacttctac
    44821 ccttacagtt gcaagccgga agatgtctgg gcggcgctgg agaaagatcg ggaaaacctg
    44881 ttttttatcg atgtgcaggc gcggggcgcg tatccggctt actctgcccg cgtatttcgc
    44941 gaaaaagggg taaccatcga caaagcaccg ggcgatgatg agatcctgaa aaacaccgtc
    45001 gattttgtct ctttcagcta ttacgcctcg cgctgcgcct cggcggagat gaacaccaac
    45061 aatagcagtg cggcgaacgt agtgaaatcg ctgcgtaacc cgtatctaca ggtgagcgac
    45121 tggggctggg gaattgatcc actcggtctg cgtatcacca tgaacatgat gtacgaccgc
    45181 tatcagaagc cgctgtttct ggtggaaaac ggcctcggcg caaaagatga acttgctgcc
    45241 aatggcgaga ttaacgatga ctatcgcatc agctatttac gcgaacatat ccgcgcaatg
    45301 ggcgaagcga ttgcagacgg cattccgctg atgggctaca ccacctgggg ctgtattgat
    45361 ttagtttccg cctctacggg tgaaatgagt aaacgctacg gttttgtcta cgtagaccgt
    45421 gacgacgcag gcaacggcac gctgacgcgc acgcgtaaga aatcgttctg gtggtataaa
    45481 aaagtgattg ccagtaatgg ggaagattta gagtaagcaa ccgtgccgga tgcggcgtga
    45541 acgccttatc cggcctacat agttcgtgcg acatgtaggc ccgataagcg cagcgcatcg
    45601 ggcaatttag catttggcat cagagctggt tcaccccatc aagaacatcc ctgtcctgat
    45661 tccttattga aaaactactc ttcttccacc gctaactgcg cgaagccgcc gttcccttcc
    45721 cagccttcca gtcgttgata aacggtttct accgcatctt taatcggctg ggtcatcggg
    45781 tagtaaaagc cgacgatatc cggctggatg ccgaggaaaa tcacttcgcc aatatcttct
    45841 ttcaactgat cgataaggta gttcaacggc atgttatggg tagtcatcat aaacatctcg
    45901 gcgatatcat ccgggtcgat gatgcggatt tcgccggggt ttagccccat atcagtagcg
    45961 tcaacaatca gtaaccgcgt agggcgcagt tcacggatgg cgacgatgtc gttttccggt
    46021 gcgctaccgc cgtcaatcac cacccagtta cctttcggcg cggcggcgca cttttccgcc
    46081 agcagcggac ctgcgccatc atcgcccatc atgctattgc caacacagag taaaacgtca
    46141 gtcacgcagt ctcctcacca tcaaatagat ggcgttttcc tgatgaatat catgcagcat
    46201 acttaacagc attgtactcc attcctgctg ctgcgaagtt tgcgctgccc gggctttatc
    46261 gaatgcattg gccagcatcg gcacatggtt aatgtcgatg acgatctcac catatttcgg
    46321 cacaccttcc attttgcggc gggcttcgct gcccgcctcc agagtggcaa tccatgccag
    46381 atattcatcc cacgggcagg tgagcgccgc ttccaggcaa tcgataaccc caaggtggtg
    46441 accaatcgcc aggctgtaat agaccacctg ctgcgcctcg gcgggcgtgg catcgttctc
    46501 atcaataaat ttacggctca gttgactgaa caccaccttt tcactcatcg gatacgcgcc
    46561 tcttcaacaa catgattcag atggctgaca atctcgttca gacgcggatc gttttccgct
    46621 tccagccagc gtgcaacctg ttcttcgccc tgccctaact gtgtaaggta atcatcggca
    46681 atctgacgac cgtaacgata acccgccagt cgacgtgctt cgcgatccac tttcacacgc
    46741 agcggctgca ccatatcgcc atgcagaatt tctgccggtt gttcatcctg ctcacccggg
    46801 ccacgggcgt gaattttctg ctccagcagg ccgagcgcca ttgcaaagcc gtacagcgtg
    46861 gccgctggcg ttggcgggca gccagggata taaacatcca ccgggacgat tttatccgta
    46921 ccgccccaca cgcagtagag atcgtggaag atcccgccac tgttaccgca ggcaccgtag
    46981 gagatacaga ttttcgggtc cggcgcggac tgccacgcac gcagcgcagg ggatcgcatt
    47041 gcacgggtga ccgcgccggt aaacagtaaa atatccgcat gacgcggtga aggaacgact
    47101 ttaatgccga agcgttctgc atcaaacagc ggcgaaagcg tggcgaaaat ttcgatttcg
    47161 cagccgttgc agccgccgca gtccacgcgg taaacgtagg cagagcgttt gatttttttc
    47221 agtaacgacg ccttcatact ggcgatggat tcatccaccg tcatcgggac cggaatgccg
    47281 ttggcgtcac gggggcctaa taaattgctc atcagatggc ctctttcata tggcgagtca
    47341 gttcaatacg gtcggacggc accaggcatt tctggcgctt acattccggg caagtctcaa
    47401 agctttcgcg gtggttttcc gcgcggctgt cgccattgtg cttaagcagc gcaatggcgt
    47461 agtcgatctc tttctggacg gcgaaaggac gattgcagac gcgacagttg cacagcgcga
    47521 agcgggactg ttgcaggaag tcttctttct tccacaccgc cagttcgtac tcttgcgaca
    47581 gcttaatcgc cgccgtcggg caaacttctt cgcagcgtcc acagaagatg caacgcccga
    47641 gattaaactc ccacgcaagc tctcctgtgg cgaggtcagt ttcaaccgtt aaggcgtttg
    47701 acgggcaggc attgacgcag gccgcgcagc cgatgcactg ctgcgggttc tgctctggct
    47761 taccacggaa gtttttatca accgcaatcg gctccagcgg ataagacgag gtcgccgtgc
    47821 cggttttgat gacttttttg ataaaggtaa acatggcgat tccttatttc agcggcgagt
    47881 ttttacgctc aatgctgtaa cgctcgagtt ctttgtacgg caccactttg ctcttcttct
    47941 tacgcacatc gaccacggtc atgcggtcgg tacaggagta gcaagggtcg aggctaccga
    48001 taatcagcgg cgcatcggaa acggtattgc cacgcagcat gtagcgcagg gtcggccagt
    48061 tcgcgtaagt cgcggcacgg cagcgccagc ggtacagctt ctggttgtcg ccggtcatgc
    48121 tccagtggat atcatcgccg cgcggcgctt cggcaaagcc cagcgcaaag cggtgcggaa
    48181 tgtaggtaaa gccttccacc atcagtgggc cacacggcag gttatccaga ccgtagtcga
    48241 tcatgttcag cgcggtatag acttcgttga tacgcacttt cagacgggaa ataacgtcgc
    48301 agccctgctc gctgtggact tccattggca gcaggccata accaacaaac gggtgatcgg
    48361 cgcgagtatc gcgagcgtga ccgctggcac ggaccatcgg gccgacgtta ctgaagtcgc
    48421 gagcgatttc cgggtccaga cgaccaatgc cgacagtgcg ttgttccatg ttcggtgtgc
    48481 tcaacagcac atccaccagc tcctgcactt cacgacgcat ctgttgcgcc agctggcggg
    48541 tctggatcat gtcatctttc agcagatcgc gacgaatccc gccgatcagg ttcaggccgt
    48601 aggttttacg cgcaccggta aggatctctg ccattttcat ggaggtttca cgcacgcgga
    48661 agaactgcat aaagccggag tcaaagccgg taaagtggca ggcaagaccg aggttgagca
    48721 gatgcgagtg caggcgttcc acctccagca gaatggcgcg gatcatctgc gcacgttctg
    48781 gcacctgaat acccatcgcg ttttccaccg acgtggtgta ggcggtgctg tgagcaaagc
    48841 cgcagatccc gcacacacgg tcagagagga aggtcacttc gttgtaaccc atgcgggttt
    48901 ccgccagttt ttccatgccg cgatggacgt agaacagacg gtagtcggcg tcgataatgt
    48961 tttcgccatc gacgaacaga cggaagtgac ccggttcgtc ggaagtgacg tgcaacggac
    49021 caatcggcac gacgttgttt ttcttgtcgc ccagttcgtt gatgaactcg taggtttcag
    49081 catcagtagt tggtgccgga cgctgacggt aatccatgct gtctttacgc agcggataaa
    49141 gttcatccgg ccagtcatcc ggcagcacca gacgacgttc atccggcaga ccaaccggaa
    49201 tcaaaccgta catatcgcgc acttcgcgct cgccccacac cgccgccgga acgcgcggcg
    49261 tcacggacgg atactccggt ttgttggcat caacttcgac gcgcaccgta atccagcact
    49321 tggtaccctt ctccatcgac agcacgtaat aaacggcgta atgaccattc agtttgcgtt
    49381 cgtcgttacc aaaaagcacc gacagccagc ccccctgctt gtagtagaga aactccacca
    49441 cttccggcag atagttcacc tttacggtaa cagtcagctg atctttggtc tgccaggcgt
    49501 ggtccagcac gacgcccgga aatgcctcat tcagcgcggc gagataatgt tgacctaatt
    49561 tttcttcaga catgctcaaa ctctctttaa tcacgccgcc agcaaggaga cgaacgctaa
    49621 aaatgcaaag ccaaacccag cccaggtaat gcgcggagta atatcaagac gcagacgggc
    49681 catgctgttt tcgaacagcg cgataaccag gacgccgacg accagtttga cgatggcaat
    49741 caccagcgcc agcagcagcc caccgacggt gaaggtttcc atttgtcccc acggaataaa
    49801 caccccgacg aacatctgca gcaccaccag ctgtttcagg ctgatacccc atttcatgac
    49861 gccaaagccg ctgccgctat actcagagag cggaccttcc tgcaactcct gctcggcttc
    49921 cgccaggtcg aacggcagtt tgcccatttc gataaaagtg gcgaacgcac aggcacaaag
    49981 cgtcagcacc agcgggatgc tctggctcag cggccagtga taaacggtgt cggtgatgtt
    50041 gctgatgttg gtggaaccgg caacctgtgc ggcaacccac agaccaagca gcagcatcgg
    50101 ttcgaccagc acgccaagca tcgcttcacg gctcgcgccg atagcagtaa acgggctacc
    50161 ggtatccaga ccggaaatgg caaagaagaa acgcgcgatg gcaaagagat acagtaaggt
    50221 gatcaaatcg cccagttgcg gcagcggaga accaacggtc accaccggca gcgcagtagc
    50281 gatagtcagc atgacgccca ccatcacata cggcgtcagg cggaacaccc agccggaggc
    50341 atccgggcca acgctctgac gccccagcag tttgataatg tcgcgatact cctgcaacac
    50401 gcccggcccg cgacggttat gcaagcgggc gcgcgccacg cgggttatac cggagagcag
    50461 cggcgcaacg gcaaataaca ccagcgcctg aattaacgga tataaaacac tcattctcag
    50521 gctcctcgtg aaacaataat caccaccagt accgccagtt caaccagcgc catccggcgg
    50581 aacagcaacg cactcccctc gcgctgccag cccggcacca gcgacaccgg attcagccat
    50641 ttgcgcagtt tcagcaccgg cgcaaacgcc tgtttcaccg gcatggcaaa accgtgagcg
    50701 gtaatcacca ttgatttttc gtggtcgtaa ccgcacaccc aggccgcacc gcgggaacgc
    50761 gatggcaaac gatcgccttt gcaaatcgcc ataatgatga atggcagcag cgggcaggca
    50821 atcagcagca acgtgatcat tggttgagaa acggtggtgt tagcaggctc cagaggcaga
    50881 ggtactgcag cagagagcat cgacagcagc cacggcgcag caacaccgcc aattacgcag
    50941 caaatcgcca gtgccactac gcttacgctc atcaggagcg gtgcacaggt ggcgttttcg
    51001 gcttctttgg tacgcggcgc gccgaggaac gtgacgccat agactttcgc catacacatc
    51061 accgccagcg caccggtaat tgccagaccc acagcgagca gcggccccag cagacgggca
    51121 acaaacgcgc cactattgct cagtttgaag aaggattgat agataaccca ttccccggca
    51181 aacccgttca gcggcggcag cgcagccatt gccatcagcc cgactaacat ggcgatggag
    51241 ataaccggca ttttcttgcc aataccaccg agtttttcga tatcgcgatg accggtacgg
    51301 aaccagacgc tccccgcccc gaggaacagt acgcttttga acaggctatg gttaagcaga
    51361 tggtacagac caccgaccag gccaagagca atcagcgccg gttgttcgag cgcgataccc
    51421 gttacgccag cgcccagccc cagcaggata atgccgatat tttccagggt gtggtaagcc
    51481 agtagacgct ggatattatg ttccatcaac gcatacagac cgccgacaaa cgcggtgatc
    51541 atgccgagca ccagcagcgc gatcccccac cacagcggtg cattaccgcc cagcagtgac
    51601 agggttaaaa tgcccagcag gccaattttc atgactaccg tagaaaacaa cgcggcagct
    51661 ggcgcagagg cgttcgcatg tgcctgcggc acccagccgt gcagcggaat aatcccggcc
    51721 agcaggccaa agccaatcac accgagcagc cagatatcgg aaccgagcgg cagctgttgc
    51781 atacgcatat ccagcaggcg cagatccagc gtgccgtaac gctgccacag cagccagcaa
    51841 gcaatcgcca gcagcagagt gccaagacgc cccagcgcaa accacagttt gccctctttg
    51901 ctgttgctgg tgaggaacac cgcacacaag gccatgactt cggccattac cacgaacatg
    51961 ccgaggttgc tggcaatgac ggcgcagacg gcggcagcca tcaacatatt gatctgcaag
    52021 ccgttgcact tcacctgcgc gtggcgatgc cagtcaatgt tgtagaggct gacaaacaga
    52081 ccgcacagac cgagcgtaat cagccaaatc gcgttaagcg gagagatttg cacatcgtag
    52141 cttaccagcg acagcgcacc gctcacgcca accgcgccag tcagtacagt gaagcccgcg
    52201 gctgccgtat acagactacc aaccgcgccg ccaattccgg ctatccagcc gcttagcgcc
    52261 ttttgaaaag aaaagagaaa tgccagaata gcggcggcga caaaccacgc cacgccgcta
    52321 ttgatcaggg aaattgcgct catttagcct ctccactttg agcctgctga aacaaggtga
    52381 gatcgccaaa gtccgtgtta aaggtcagct cacgcttacg tttgctgacg cgggcgatat
    52441 cggtgttatc caccagatgc agggctttag tcgggcacat ccgcacgcag gccggacctt
    52501 gttcatcaaa gctacaaagg tcacatttga cggcgatcgc gcgaatacct ggcacccagt
    52561 caagcaatgt gctgacacgc gccggagcag gcggtgccgg tggcgctttc ggggtattgg
    52621 cgtttgccgg aatatccagc ggacggctgc cggaaaattc aattgcgcca aacgggcagg
    52681 cgatgccaca cagtttgcag cttacgcaca ggctttcatt caactgcacg gccccatcaa
    52741 cacgggtgat ggcgttaacc gggcagacca ccgcgcaggg tgcatcttca cagtggtgac
    52801 agagctgcgg cgcagattct ttttcattta gcatcactct caggcgcggc attgattgca
    52861 ggccgtgctg gcgatgcgtc tctgaacagg cggcctcaca agtgtggcag ccgatacaga
    52921 gcgtggagtc agcaattaca aaacgattca ccaggcattc ctcaggtgat tgtcattttt
    52981 gacgaaaaca tgccgatgaa atgtcatttt cgacactcat cgacacgccc atccccaaac
    53041 aggcgtaacg cctgcaaaac gggcaaagcc tcagctcatg ctgccgggct ttgtcccttt
    53101 accagttggc ttaaattcac cggcacattg ttttcaatag cggtgtataa gctgtcgtaa
    53161 acgccagcga ttttttcgag atagtgttcc agaacctgtt cgcgatcgcg gatgttgacc
    53221 ttgccatcaa tcatgccgtc aatgctcagt tgcgcctgcg caatccgctg tttgtcttcg
    53281 ccatcttttg tttcgacgta atactcgatg gtgtggctgt taaagccatc cgccagggtg
    53341 acgtaaatac gaaaatgttc aaaaaggacg aaacagagtt ctttgtccgg cgcgcagctc
    53401 atggcgtgat gcaggcgcgc tttcgataac gtgatcccct gaaccagcga attgcagtag
    53461 atgtgccact ggtcctgtag gcgacgatgc cgctgtgcga tgtaatcggc tttctcgctt
    53521 atttcccaaa tagtcattgt caggttaccc gtttaacaga gatgccagct ttaagcattt
    53581 tttgtgccaa cttttaattt attgttatta aagcgatttt taagattttg gtgaatttcg
    53641 tcgccgtgtc gacgtgtcat ttcgacatca tcgacattat tcactgcagg gataatcaac
    53701 actggcacaa ttattgcttg tagctggcaa tagttaatgg gaggcgatat gcacgaaata
    53761 accctctgcc aacgggcact ggaattgatc gaacagcagg ccgcaaaaca cggcgcaaaa
    53821 cgcgtaactg gggtctggct caaaattggc gcattttctt gtgtcgaaac cagctctctt
    53881 gccttttgtt ttgatctggt ttgccgcggc agcgtggcgg aaggttgtaa actgcacctc
    53941 gaagaacaag aggccgaatg ctggtgtgaa acatgccaac agtatgtgac gctactgacc
    54001 cagcgcgtcc gccgctgtcc actgtgtcat ggtgacatgc tacagattgt ggcagacgac
    54061 ggtttacaga ttcggcggat agaaatagac caggagtgag cgatgtgtac aacatgcggt
    54121 tgcggtgaag gcaacctgta tatcgagggt gatgaacata accctcattc cgcgtttcgt
    54181 agcgcgccat ttgccccggc ggcacgcccg aagatgaaaa tcaccggcat taaaacgcct
    54241 gaatttaccc ccagccagac tgaagaaggc gacctgcatt acggtcatgg cgaagcgggc
    54301 actcacgcac cgggcatgag ccagcgtcgg atgctggaag tcgaaattga cgtgctggac
    54361 aaaaataacc gtctggctga acgcaaccgc gcgcgctttg ctgcccgcaa gcaactggtg
    54421 ctcaacctgg tttccagccc aggttccggt aaaaccaccc tgctgacgga aaccttaatg
    54481 cgcctgaaag acagcgttcc gtgcgcggtt attgaaggtg accagcaaac cgtgaacgat
    54541 gccgcacgca ttcgcgctac cggcacacca gcgattcagg tgaataccgg taaaggctgc
    54601 catcttgacg cacagatgat tgctgacgcc gcaccgcgcc tgccactgga cgataacggt
    54661 attctgttta tcgaaaacgt tggcaacctc gtatgcccgg cgagcttcga tctcggtgaa
    54721 aaacacaaag tggcggtgct ttccgttacc gaaggtgaag acaaaccgct gaaatatccg
    54781 catatgtttg ccgccgcctc gctgatgctg ctcaacaaag ttgacctgtt gccgtatctc
    54841 aactttgacg ttgagaagtg catcgcctgc gcccgcgaag tcaatccaga aattgaaatc
    54901 atccttattt ccgccaccag cggcgaaggg atggaccagt ggctgaactg gctggagaca
    54961 cagcgatgtg cataggcgtt cccggccaga tccgcaccat tgacggtaac caggcgaaag
    55021 tcgacgtctg cggcattcag cgcgatgtcg atttaacgtt agtcggcagc tgcgatgaaa
    55081 acggtcagcc gcgcgtgggc cagtgggtac tggttcacgt tggctttgcc atgagcgtaa
    55141 ttaatgaagc cgaagcacgc gacactctcg acgccttaca aaacatgttt gacgttgagc
    55201 cggatgtcgg cgcgctgttg tatggcgagg aaaaataatg cgttttgttg atgaatatcg
    55261 cgcgccggaa caggtgatgc agttaattga gcatctgcgc gaacgtgctt cacatctctc
    55321 ttacaccgcc gaacgccctc tgcggattat ggaagtgtgc ggtggtcata cccacgccat
    55381 ttttaaattc ggcctcgacc agttactgcc tgaaaacgtt gagtttatcc acggtccggg
    55441 ttgcccggtg tgcgtactgc cgatgggcag aatcgacacc tgcgtggaga ttgccagcca
    55501 tccggaagtc atcttctgta cctttggcga cgccatgcgc gtgccgggga aacagggatc
    55561 gctgttgcag gcaaaagcac gcggtgccga tgtgcgcatc gtttactcgc cgatggatgc
    55621 gttgaaactg gcgcaggaga atccaacccg caaagtggtg ttcttcggct taggttttga
    55681 aaccaccatg ccaaccaccg ctatcactct gcaacaggcg aaagcgcgtg atgtgcagaa
    55741 tttttacttc ttctgccagc acattacgct tatcccgacg cttcgcagtt tgctggaaca
    55801 gccggataac ggtatcgacg cgttcctcgc gccgggccac gttagtatgg ttatcggcac
    55861 tgatgcctat aattttatcg ccagcgattt tcatcgtccg ctggtggtcg ctggtttcga
    55921 accccttgat ctactgcaag gcgtggtgat gctggtggag cagaaaatag cggcccacag
    55981 caaggtagag aatcagtatc gtcgggtggt accggatgcc ggtaacctgc tggcgcaaca
    56041 ggcgattgcc gatgtgttct gtgtcaacgg cgacagcgaa tggcgcggct taggcgtgat
    56101 tgaatcttct ggcgtgcacc tgacgccgga ttatcaacga ttcgatgccg aagcacattt
    56161 ccgcccggca ccgcagcagg tctgcgatga cccgcgcgcg cgttgtggcg aagtcttgac
    56221 gggcaaatgt aagccgcatc aatgcccgct gtttggtaac acctgtaatc ctcaaaccgc
    56281 gtttggtgcg ctgatggttt cctccgaagg agcgtgcgcc gcgtggtatc agtatcgtca
    56341 gcaggagagt gaagcgtgaa taatatccaa ctcgcccacg gcagcggcgg ccaggcgatg
    56401 cagcaattaa tcaacagcct gtttatggaa gcctttgcca acccgtggct ggcagagcag
    56461 gaagatcagg cacgtcttga tctggcgcaa ctgatagcgg aaggcgaccg tctggcgttc
    56521 tccaccgaca gttacgttat tgacccgctg ttcttccctg gcggtaatat cggcaagctg
    56581 gcgatttgcg gcacagccaa tgacgttgcg gtcagtggtg ccattccgcg ctatctctcc
    56641 tgtggcttta tcctcgaaga aggattgccg atggagacac tgaaagccgt agtgaccagc
    56701 atggcagaaa ccgcccgcac ggcaggcatt gccatcgtta ctggcgatac taaagtggtg
    56761 cagcgcggcg cggcagataa actgtttatc aacaccgcgg gcatgggcgc aattccggcg
    56821 aatattcact ggggcgcaca gacgctaacc gcaggcgatg tattgctggt gagcggtaca
    56881 ctcggcgacc acggggcgac tatccttaac ctgcgtgagc agctggggct ggatggcgaa
    56941 ctggtcagcg actgcgcggt gctgacgccg cttattcaga cgctgcgtga cattcccggc
    57001 gtgaaagcgc tgcgtgatgc cacccgtggt ggtgtaaacg cggtggttca tgagttcgcg
    57061 gcagcctgcg gttgcggtat tgaaatttct gaatcagcgc tgccggttaa acctgccgtg
    57121 cgcggcgttt gcgaattgct gggactggac gccctgaact ttgccaacga aggcaaactg
    57181 gtgatcgccg ttgaacgcaa cgcggcagag caagtgctgg cagcgttaca ttcccatcca
    57241 ctggggaaag acgcggcgct gattggtgaa gtggtggaac gtaaaggtgt tcgtcttgcc
    57301 ggtctgtatg gcgtgaaacg aaccctcgat ttaccacacg ccgaaccgct tccgcgtata
    57361 tgctaataaa attctaaatc tcctatagtt agtcaatgac cttttgcacc gctttgcggt
    57421 gctttcctgg aagaacaaaa tgtcatatac accgatgagt gatctcggac agcaagggtt
    57481 gttcgacatc actcggacac tattgcagca gcccgatctg gcctcgctgt gtgaggctct
    57541 ttcgcaactg gtaaagcgtt ctgcgctcgc cgacaacgcg gctattgtgt tgtggcaagc
    57601 gcagactcaa cgtgcgtctt attacgcatc gcgtgaaaaa gacaccccca ttaaatatga
    57661 agacgaaact gttctggcac acggtccggt acgcagcatt ttgtcgcgcc ctgatacgct
    57721 gcattgcagt tacgaagaat tttgtgaaac ctggccgcag ctggccgcag gtgggctata
    57781 cccaaaattt ggtcactatt gcctgatgcc actggcggcg gaagggcata tttttggtgg
    57841 ctgtgaattt attcgttatg acgatcgccc ctggagcgaa aaagagttca atcgtctgca
    57901 aacatttacg cagatcgttt ctgtcgtcac cgaacaaatc cagagtcgcg tcgttaacaa
    57961 tgtcgactat gagttgttat gccgggaacg agataacttc cgcatcctgg tcgccatcac
    58021 caacgcggtg ctttcccgcc tggatatgga cgaactggtc agcgaagtcg ccaaagaaat
    58081 ccattactat ttcgatattg acgatatcag tatcgtctta cgcagccacc gtaaaaacaa
    58141 actcaacatc tactccactc actatcttga taaacagcat cccgcccacg aacagagcga
    58201 agtcgatgaa gccggaaccc tcaccgaacg cgtgttcaaa agtaaagaga tgctgctgat
    58261 taatctccac gagcgggatg atttagcccc ctatgaacgc atgttgttcg acacctgggg
    58321 caaccagatt caaaccttgt gcctgttacc gctgatgtct ggcgacacca tgctgggcgt
    58381 gctgaaactg gcgcaatgtg aagagaaagt gtttaccact accaatctga atttactgcg
    58441 ccagattgcc gaacgtgtgg caatcgctgt cgataacgcc ctcgcctatc aggaaatcca
    58501 tcgtctgaaa gaacggctgg ttgatgaaaa cctcgccctg accgagcagc tcaacaatgt
    58561 tgatagtgaa tttggcgaga ttattggccg cagcgaagcc atgtacagcg tgcttaaaca
    58621 agttgaaatg gtggcgcaaa gtgacagtac cgtgctgatc ctcggtgaaa ctggcacggg
    58681 taaagagctg attgcccgtg ctatccataa tctcagtggg cgtaataatc gccgcatggt
    58741 caaaatgaac tgcgcggcga tgcctgccgg attgctggag agcgatctgt ttggtcatga
    58801 gcgtggggct tttactggtg ccagcgccca gcgcattggt cgttttgaac tggcggataa
    58861 aagctccctg ttcctcgacg aagtgggcga tatgccactg gagttacagc cgaagttgct
    58921 gcgtgtattg caggaacagg agtttgaacg cctcggcagc aacaaaatca ttcagacgga
    58981 cgtgcgtcta atcgccgcga ctaaccgcga tctgaaaaaa atggtcgccg accgtgagtt
    59041 ccgtagcgat ctctattacc gcctgaacgt attcccgatt cacctgccgc cactacgcga
    59101 gcgtccggaa gatattccgc tgctggcgaa agcctttacc ttcaaaattg cccgtcgtct
    59161 ggggcgcaat atcgacagca ttcctgccga gacgctgcgc accttgagta atatggagtg
    59221 gccgggtaac gtacgcgaac tggaaaacgt cattgagcgc gcggtattgc taacacgcgg
    59281 caacgtgctg cagctgtcat tgccagatat tgctttaccg gagcctgaaa cgccgcctgc
    59341 cgcaacggtt gtcgcccagg agggcgaaga tgaatatcag ttgattgttc gcgtgctgaa
    59401 agaaactaac ggcgtggttg ccgggcctaa aggcgctgcg caacgtctgg ggctgaaacg
    59461 cacgaccctg ctgtcacgga tgaagcgact gggaattgat aaatcggcat tgatttaaca
    59521 gatgtggtta tactcgatca acattcaatg aaaggaggca gtatggcagt ttcagcaaga
    59581 aatcaattaa ccggaacagt tagcgcagta gcaatgggcg cggtgaatga cgaagtagaa
    59641 ctgacgctgg caggtggtgc aaagctggtg gcaattgtga ctcacagcag ccagcaggct
    59701 ctgggtctgg cgaaagggaa agaagcgatt gccctgatta aagccccgtg ggtaacgctg
    59761 gcaacggaag actgtggact gaaattctct gctcgcaacc agtttgctgg cagcgtgtcg
    59821 acaatcaccg aaggcgcagt gaacgccact gtccatatca aaacggatgc gggttttgaa
    59881 attgtggcag tggtaacaaa tgaaagtcag gatgaaatga aactcaccac tggctcacgc
    59941 gttattgcat tgatcaaagc atcagccatc ctgattgcca ccaaagcgta aacgccttat
    60001 ttgccggaga gtatggctct ccggcagatt tatttctttg gacgatattt ttctggcaac
    60061 tccggcaccg gacgcttgtc atcgatgaga tgacgcacgg ttaagatcgg atgacgccac
    60121 agcattctcg gcccggccca acgcataatc tgtttcatct cttcacgctt tgcaggctgg
    60181 taacagtgca ccggacactg cttacaggct ggtttctctt cgccgaacac acatttatcc
    60241 agccgctttt gcgcgtaagc aaacaacgcc tcgtaatgct ccggctccgc tgacgcctgc
    60301 gggcatttcg cttgataaag atcgatcatt tttttaatcg tcagtttttc acgagagata
    60361 cgcttgccgg acatgctgcc tccatctcat taagatgcat ttatattaca acttaatctt
    60421 aaagggcact atgactctaa agaagaaggg ttcgccaacc gatacaattt tgcgtacttg
    60481 cttcataagc atcacgcaaa agctgcaaaa cagcatcttt cccggaacca gcatcaagaa
    60541 ctcgccgttc gcttcttccc ctgaaatgat taactccggt atcatgtgcg ccttatgtga
    60601 ttacaacgaa aataaaaacc atcacacccc atttaatatc agggaaccgg acataacccc
    60661 atgagtgcaa tagaaaattt cgacg
//
LOCUS       DAZFFG010000016        57618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFFG010000016 DAZFFG010000000
VERSION     DAZFFG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.016"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            74..616
                     /gene="zapC"
                     /locus_tag="ACHJ7J_001286"
     CDS             74..616
                     /gene="zapC"
                     /locus_tag="ACHJ7J_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO4999546.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(613..1722)
                     /gene="ycbX"
                     /locus_tag="ACHJ7J_001287"
     CDS             complement(613..1722)
                     /gene="ycbX"
                     /locus_tag="ACHJ7J_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO4999547.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRNLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            1966..4074
                     /gene="rlmKL"
                     /locus_tag="ACHJ7J_001288"
     CDS             1966..4074
                     /gene="rlmKL"
                     /locus_tag="ACHJ7J_001288"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO4999548.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTTDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4086..5993
                     /gene="uup"
                     /locus_tag="ACHJ7J_001289"
     CDS             4086..5993
                     /gene="uup"
                     /locus_tag="ACHJ7J_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4999549.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6123..7376
                     /gene="pqiA"
                     /locus_tag="ACHJ7J_001290"
     CDS             6123..7376
                     /gene="pqiA"
                     /locus_tag="ACHJ7J_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO4999550.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7381..9021
                     /gene="pqiB"
                     /locus_tag="ACHJ7J_001291"
     CDS             7381..9021
                     /gene="pqiB"
                     /locus_tag="ACHJ7J_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO4999551.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9018..9581
                     /gene="pqiC"
                     /locus_tag="ACHJ7J_001292"
     CDS             9018..9581
                     /gene="pqiC"
                     /locus_tag="ACHJ7J_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO4999552.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9837..10004
                     /gene="rmf"
                     /locus_tag="ACHJ7J_001293"
     CDS             9837..10004
                     /gene="rmf"
                     /locus_tag="ACHJ7J_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO4999553.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10074..10592)
                     /gene="fabA"
                     /locus_tag="ACHJ7J_001294"
     CDS             complement(10074..10592)
                     /gene="fabA"
                     /locus_tag="ACHJ7J_001294"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO4999554.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10661..12421)
                     /gene="ycbZ"
                     /locus_tag="ACHJ7J_001295"
     CDS             complement(10661..12421)
                     /gene="ycbZ"
                     /locus_tag="ACHJ7J_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4999555.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAV
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12607..13059
                     /gene="matP"
                     /locus_tag="ACHJ7J_001296"
     CDS             12607..13059
                     /gene="matP"
                     /locus_tag="ACHJ7J_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO4999556.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            complement(13135..14175)
                     /gene="ompA"
                     /locus_tag="ACHJ7J_001297"
     CDS             complement(13135..14175)
                     /gene="ompA"
                     /locus_tag="ACHJ7J_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO4999557.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14532..15041)
                     /gene="sulA"
                     /locus_tag="ACHJ7J_001298"
     CDS             complement(14532..15041)
                     /gene="sulA"
                     /locus_tag="ACHJ7J_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO4999558.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLPPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15260..15889
                     /gene="sxy"
                     /locus_tag="ACHJ7J_001299"
     CDS             15260..15889
                     /gene="sxy"
                     /locus_tag="ACHJ7J_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO4999559.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     NGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15852..18014)
                     /gene="yccS"
                     /locus_tag="ACHJ7J_001300"
     CDS             complement(15852..18014)
                     /gene="yccS"
                     /locus_tag="ACHJ7J_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO4999560.1"
                     /translation="MAYMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18024..18470)
                     /gene="yccF"
                     /locus_tag="ACHJ7J_001301"
     CDS             complement(18024..18470)
                     /gene="yccF"
                     /locus_tag="ACHJ7J_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO4999561.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18593..20647
                     /gene="helD"
                     /locus_tag="ACHJ7J_001302"
     CDS             18593..20647
                     /gene="helD"
                     /locus_tag="ACHJ7J_001302"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO4999562.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRGLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20679..21137)
                     /gene="mgsA"
                     /locus_tag="ACHJ7J_001303"
     CDS             complement(20679..21137)
                     /gene="mgsA"
                     /locus_tag="ACHJ7J_001303"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO4999563.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21233..21895)
                     /gene="yccT"
                     /locus_tag="ACHJ7J_001304"
     CDS             complement(21233..21895)
                     /gene="yccT"
                     /locus_tag="ACHJ7J_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO4999564.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22068..22481
                     /gene="yccU"
                     /locus_tag="ACHJ7J_001305"
     CDS             22068..22481
                     /gene="yccU"
                     /locus_tag="ACHJ7J_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO4999565.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22526..22843)
                     /gene="hspQ"
                     /locus_tag="ACHJ7J_001306"
     CDS             complement(22526..22843)
                     /gene="hspQ"
                     /locus_tag="ACHJ7J_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO4999566.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22901..24091)
                     /gene="rlmI"
                     /locus_tag="ACHJ7J_001307"
     CDS             complement(22901..24091)
                     /gene="rlmI"
                     /locus_tag="ACHJ7J_001307"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO4999567.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24186..24464
                     /gene="yccX"
                     /locus_tag="ACHJ7J_001308"
     CDS             24186..24464
                     /gene="yccX"
                     /locus_tag="ACHJ7J_001308"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO4999568.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLSGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24461..24790)
                     /gene="tusE"
                     /locus_tag="ACHJ7J_001309"
     CDS             complement(24461..24790)
                     /gene="tusE"
                     /locus_tag="ACHJ7J_001309"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO4999569.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24881..25540)
                     /gene="yccA"
                     /locus_tag="ACHJ7J_001310"
     CDS             complement(24881..25540)
                     /gene="yccA"
                     /locus_tag="ACHJ7J_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO4999570.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25747..25834)
                     /locus_tag="ACHJ7J_001311"
     tRNA            complement(25747..25834)
                     /locus_tag="ACHJ7J_001311"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25798..25800),aa:Ser,seq:tga)
     gene            26260..27378
                     /gene="hyaA"
                     /locus_tag="ACHJ7J_001312"
     CDS             26260..27378
                     /gene="hyaA"
                     /locus_tag="ACHJ7J_001312"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO4999571.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            27375..29168
                     /gene="hyaB"
                     /locus_tag="ACHJ7J_001313"
     CDS             27375..29168
                     /gene="hyaB"
                     /locus_tag="ACHJ7J_001313"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO4999572.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLIDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPKECRGVGFTEAP
                     RGALGHWAAIRDGKLDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGNELISVQVR"
     gene            29187..29894
                     /gene="hyaC"
                     /locus_tag="ACHJ7J_001314"
     CDS             29187..29894
                     /gene="hyaC"
                     /locus_tag="ACHJ7J_001314"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO4999573.1"
                     /translation="MQEKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSTGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWHKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            29891..30478
                     /gene="hyaD"
                     /locus_tag="ACHJ7J_001315"
     CDS             29891..30478
                     /gene="hyaD"
                     /locus_tag="ACHJ7J_001315"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO4999574.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            30475..30873
                     /gene="hyaE"
                     /locus_tag="ACHJ7J_001316"
     CDS             30475..30873
                     /gene="hyaE"
                     /locus_tag="ACHJ7J_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO4999575.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            30870..31727
                     /gene="hyaF"
                     /locus_tag="ACHJ7J_001317"
     CDS             30870..31727
                     /gene="hyaF"
                     /locus_tag="ACHJ7J_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO4999576.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVNALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            31861..33405
                     /gene="appC"
                     /locus_tag="ACHJ7J_001318"
     CDS             31861..33405
                     /gene="appC"
                     /locus_tag="ACHJ7J_001318"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO4999577.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLMAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SSAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            33417..34553
                     /gene="appB"
                     /locus_tag="ACHJ7J_001319"
     CDS             33417..34553
                     /gene="appB"
                     /locus_tag="ACHJ7J_001319"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4999578.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            34566..34658
                     /gene="cbdX"
                     /locus_tag="ACHJ7J_001320"
     CDS             34566..34658
                     /gene="cbdX"
                     /locus_tag="ACHJ7J_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO4999579.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            34738..36036
                     /gene="appA"
                     /locus_tag="ACHJ7J_001321"
     CDS             34738..36036
                     /gene="appA"
                     /locus_tag="ACHJ7J_001321"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO4999580.1"
                     /translation="MKAILIPFLSLLIPLTPQSTFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFAQIVNEARIPACSL"
     gene            complement(36156..38336)
                     /gene="etk"
                     /locus_tag="ACHJ7J_001322"
     CDS             complement(36156..38336)
                     /gene="etk"
                     /locus_tag="ACHJ7J_001322"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO4999581.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(38356..38802)
                     /gene="etp"
                     /locus_tag="ACHJ7J_001323"
                     /gene_synonym="yccY"
     CDS             complement(38356..38802)
                     /gene="etp"
                     /locus_tag="ACHJ7J_001323"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO4999582.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(38790..39929)
                     /gene="gfcE"
                     /locus_tag="ACHJ7J_001324"
     CDS             complement(38790..39929)
                     /gene="gfcE"
                     /locus_tag="ACHJ7J_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO4999583.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(39975..42071)
                     /gene="gfcD"
                     /locus_tag="ACHJ7J_001325"
     CDS             complement(39975..42071)
                     /gene="gfcD"
                     /locus_tag="ACHJ7J_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4999584.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(42071..42817)
                     /gene="gfcC"
                     /locus_tag="ACHJ7J_001326"
     CDS             complement(42071..42817)
                     /gene="gfcC"
                     /locus_tag="ACHJ7J_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4999585.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLAVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(42814..43458)
                     /gene="gfcB"
                     /locus_tag="ACHJ7J_001327"
     CDS             complement(42814..43458)
                     /gene="gfcB"
                     /locus_tag="ACHJ7J_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO4999586.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDIIWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(43565..43870)
                     /locus_tag="ACHJ7J_001328"
     CDS             complement(43565..43870)
                     /locus_tag="ACHJ7J_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999587.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(44312..44524)
                     /gene="cspH"
                     /locus_tag="ACHJ7J_001329"
     CDS             complement(44312..44524)
                     /gene="cspH"
                     /locus_tag="ACHJ7J_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO4999588.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            44810..45022
                     /gene="cspG"
                     /locus_tag="ACHJ7J_001330"
     CDS             44810..45022
                     /gene="cspG"
                     /locus_tag="ACHJ7J_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO4999589.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            45033..45221
                     /gene="ymcF"
                     /locus_tag="ACHJ7J_001331"
     CDS             45033..45221
                     /gene="ymcF"
                     /locus_tag="ACHJ7J_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO4999590.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            45196..45426
                     /gene="ymcE"
                     /locus_tag="ACHJ7J_001332"
     CDS             45196..45426
                     /gene="ymcE"
                     /locus_tag="ACHJ7J_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO4999591.1"
                     /translation="MHRWISQNNIRLPRGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            45416..45589
                     /gene="gnsA"
                     /locus_tag="ACHJ7J_001333"
     CDS             45416..45589
                     /gene="gnsA"
                     /locus_tag="ACHJ7J_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO4999592.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(45638..46711)
                     /gene="yccM"
                     /locus_tag="ACHJ7J_001334"
     CDS             complement(45638..46711)
                     /gene="yccM"
                     /locus_tag="ACHJ7J_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4999593.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRYMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(46783..49527)
                     /gene="torS"
                     /locus_tag="ACHJ7J_001335"
     CDS             complement(46783..49527)
                     /gene="torS"
                     /locus_tag="ACHJ7J_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO4999594.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTLEVGSCFCLRLPLRVATAPAPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLEHYL
                     QLQVNNDIPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            49610..50638
                     /gene="torT"
                     /locus_tag="ACHJ7J_001336"
     CDS             49610..50638
                     /gene="torT"
                     /locus_tag="ACHJ7J_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO4999595.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQSSTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(50611..51303)
                     /gene="torR"
                     /locus_tag="ACHJ7J_001337"
     CDS             complement(50611..51303)
                     /gene="torR"
                     /locus_tag="ACHJ7J_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO4999596.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQVQPLTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            51433..52605
                     /gene="torC"
                     /locus_tag="ACHJ7J_001338"
     CDS             51433..52605
                     /gene="torC"
                     /locus_tag="ACHJ7J_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO4999597.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGISLIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            52605..55151
                     /gene="torA"
                     /locus_tag="ACHJ7J_001339"
     CDS             52605..55151
                     /gene="torA"
                     /locus_tag="ACHJ7J_001339"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO4999598.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LKNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRHGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVDQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            55148..55747
                     /gene="torD"
                     /locus_tag="ACHJ7J_001340"
     CDS             55148..55747
                     /gene="torD"
                     /locus_tag="ACHJ7J_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO4999599.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQLAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(55899..56204)
                     /gene="cbpM"
                     /locus_tag="ACHJ7J_001341"
     CDS             complement(55899..56204)
                     /gene="cbpM"
                     /locus_tag="ACHJ7J_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO4999600.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVPHP"
     gene            complement(56204..57124)
                     /gene="cbpA"
                     /locus_tag="ACHJ7J_001342"
     CDS             complement(56204..57124)
                     /gene="cbpA"
                     /locus_tag="ACHJ7J_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO4999601.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            57385..>57618
                     /locus_tag="ACHJ7J_001343"
     CDS             57385..>57618
                     /locus_tag="ACHJ7J_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999602.1"
                     /translation="MGSNLHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKQLYEMQN"
BASE COUNT        14132 a        14456 c        15178 g        13852 t
ORIGIN      
        1 ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt tttataaggt
       61 tggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt acgatgaaga
      121 gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac gttttgcgcg
      181 caaaatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg acgccgcgct
      241 ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac aaaaagccga
      301 actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc cgaaaagctg
      361 gcattttgtt tcccatggcg aaatgtgggt gcccatgccg ggggatgcgg cctgcgtttg
      421 gttgagcgac actaacgagc aagtgaattt actggtcgtt gaatctggcg agaatgccgc
      481 gctatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc agttgggcga
      541 cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca gcttcagcct
      601 cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc tacagcaaag
      661 aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc cttctaaaag
      721 ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac gaattccctg
      781 attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct gtccctgcca
      841 gtcaatatct acattggcgt ccggttgttg cgtgatgttg gcagtatcat cagcggcagc
      901 tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc caacgcgaat
      961 cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt tatcctgggc
     1021 agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt gccctttttc
     1081 tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa acaccacatc
     1141 accaatacga atcactttcc agcgatcttc ttcccaggct gacgcgccgg aaaccaccag
     1201 attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga gattacgtaa
     1261 cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacaggg gtacagtgtt
     1321 gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca cttcgcggga
     1381 gaaaaatcca cttaaccatt tgttgatcgc ttctggcgca attcgcgcgg taaaatgtgt
     1441 gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa catatgcgct
     1501 actgccatct ggtgcggtga gatgcaagcc atcatgcacg ggtgaaggag taaatcgtac
     1561 catctgggga aactggcgag cggtgataaa cgtaccgtca ggttccgtga tcataaagat
     1621 gcgatcgaag gccagaccac tgacatctgc cagagcatgt gtaagaccaa tgccgcgcat
     1681 cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt cctcaaatga
     1741 aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc aacaattttc
     1801 ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt gtgctcgaaa
     1861 tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag actgactgca
     1921 acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa ttctctgttt
     1981 gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa cctgggggcc
     2041 gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg gcttgtttac
     2101 cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg cgagtgtaag
     2161 gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac agagatgttt
     2221 aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat ccgcaacagt
     2281 cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg gaaaaatctg
     2341 ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg gctgcataaa
     2401 gaaaccgcca gtatcgccct tgatctcagt ggtgatggtt tacatctgcg tggctatcgc
     2461 gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt gatgcgatcc
     2521 ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac gttgctgatt
     2581 gaagcggcga tgctggcgac cgatcgcgca ccaggcttgc accgtgggcg ttggggcttt
     2641 agcggctggg cgcagcatga tgaagctatc tggcaggaag tgaaagcgga agcgcaaact
     2701 cgcgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga cagcgacgca
     2761 cgggtgattc aacgtgcacg cactaacgcc cgtcttgcgg ggattggtga actgatcacc
     2821 tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc gtacggcaca
     2881 gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct gattgcgctg
     2941 catagcctgc tgggccgaat catgaaaaac cagttcggtg gctggaatct ctctttgttt
     3001 agtgcctcgc cggatctgct cagctgcctg cagctgcgtg cagacaaaca gtacaaggcg
     3061 aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgcagaaag cactacagac
     3121 agcaaaccgg cgatggtggc tgaagactac gccaatcgcc tgcgtaagaa cctcaaaaaa
     3181 ttcgagaagt gggctcgcca ggaagggatt gaatgttacc gcctgtatga cgccgatctg
     3241 ccggaatata acgttgccgt tgaccgttat gctgactggg tggtggtgca ggagtatgcg
     3301 ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat tatcgctgca
     3361 accatttcag tactggggat tgcgccaaac aaactggtgc tgaaaacccg tgaacgccag
     3421 aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttctgga agttaccgaa
     3481 tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct gttcctcgat
     3541 caccgtatcg cccgccgtat gctcggccag atgagcaaag gcaaagattt cctcaacctg
     3601 ttctcttata ccggcagcgc caccgtgcat gcgggattag gcggtgcacg cagcaccacc
     3661 accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg tctgaatggc
     3721 ctaaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct gcgtgaggca
     3781 aatgaacagt tcgatctgat ctttatcgat ccgccaacct tctctaactc aaaacgaatg
     3841 gaagatgcgt ttgatgttca gcgcgatcat ctggcgctga tgaaagattt gaaacgtctg
     3901 ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg tatggatctc
     3961 gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac gctctcccag
     4021 gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc ctgaaaggaa
     4081 tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac gcgccgcttc
     4141 tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg ggccgcaacg
     4201 gcgcaggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg gatgacggtc
     4261 gcattattta cgagcaggat ttgattgtag cgcgtctgca acaggatccg ccgcgtaacg
     4321 ttgagggtag tgtttatgat ttcgttgccg aaggcattga agaacaagcg gaatacctga
     4381 aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa aatctcaacg
     4441 aactggcaaa ggttcaggaa cagctggatc accacaacct gtggcagctg gaaaaccgca
     4501 tcaacgaagt gctggcgcaa ctggggttag atcctaacgt tgcgctgtcg tcgctttccg
     4561 gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagcaatccg cgcgtattgc
     4621 tgctggacga accgactaac cacctggata tcgaaaccat cgactggctg gaagggttcc
     4681 tcaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt atccgcaata
     4741 tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatccg ggcaattacg
     4801 atcagtacct gctggaaaaa gaagaagccc tgcgcgtgga agaactacaa aatgccgagt
     4861 tcgatcgcaa actggcgcag gaagaagtgt ggattcgcca ggggatcaaa gcacgccgta
     4921 cccgtaatga aggccgcgta cgcgccctga aagcgatgcg tcgcgagcgt ggcgaacgtc
     4981 gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc ggtaagatcg
     5041 ttttcgaaat ggaagacgtt tgctaccagg ttaacggtaa gcaactggtg aaagattttt
     5101 ctgcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg tgcggcaaaa
     5161 ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt attcacgttg
     5221 gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaacttgat cccgataaaa
     5281 cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc aagccacgcc
     5341 acgttttggg ctatttgcag gacttcctgt tccatccgaa acgggcgatg acgccagtac
     5401 gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc ctcaaaccaa
     5461 gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg ctggagctgc
     5521 tggaagaact gatcgacagc taccagggca cggtattgct ggtaagccac gatcgtcagt
     5581 ttgttgataa caccgttaca gaatgctgga tcttcgaagg cggcggtaaa attggtcgtt
     5641 atgtcggcgg ttatcatgac gcccgtggtc agcaagagca gtatgtggcg ctcaaacagc
     5701 ctgcggtgaa aaaaaacgaa gaagccgccg cgccaaaagc agaaactgta aaacgcagca
     5761 gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa ttgctcgaag
     5821 atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc ttcttcagtc
     5881 agccgcatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag caggagctgg
     5941 agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc tgatcgcaaa
     6001 atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa ctgggtttat
     6061 caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat taaggagtac
     6121 caatgtgcga acatcatcat gccgcgaagc acatcctatg ctcgcagtgt gacatgctgg
     6181 tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt ggcacaacgt
     6241 taaccgtggc gtgggatgcc ccccggcagc gtcctaccgc ctatgcgttg gctgcactgt
     6301 tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca ggagttacca
     6361 gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac gccagcctcg
     6421 gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata accattctgt
     6481 tactggtgaa tcgcgcggaa ttaccggttc gtttaaaaga gcaactggca cgggtgcttt
     6541 ttcaactcaa aacctgggga atggcggaga ttttcctcgc gggtgtgctg gtcagtttcg
     6601 ttaaactgat ggcttacggc agcattggcg taggcagcag ctttctcccc tggtgtttat
     6661 tttgtgtcct gcaactgcgt gcttttcagt gcgttgatcg tcgctggtta tgggacgata
     6721 tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg atacgtcagg
     6781 ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc gtgtgcccgc
     6841 gctgtggtac taaagggtac gttcgacgta gaaacagcct gcaatggaca ctcgcgctgc
     6901 ttgttacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg gtgacggatt
     6961 tattaggctc gaagatgcca tcgacgattc tcgctggggt catcctgtta tggagcgaag
     7021 gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca acgttaaaga
     7081 tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc gacagtgaaa
     7141 gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg attgacgttt
     7201 tcgttatcgc cgtgctctcg gcgctggtgc gtatgggagg tttaatgagt atttatccgg
     7261 caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct gctatgacgt
     7321 ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag tcctgacagt
     7381 atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc tcccgtgtgg
     7441 atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca ttacagccat
     7501 cagggaccgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg tggcaaaacc
     7561 accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact cgctgatgat
     7621 ttgacacacg ttgaaatcaa agcgcggctg aattccggta tggaaaaatt gctgcataaa
     7681 gataccgtct tttgggtggt gaaaccgcag attggtcgag aagggattag cggtctggga
     7741 accctgctgt ctggggttta tatcgaactg cagccaggcg cgaaaggcag caaaatggat
     7801 aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg tatccgtgtg
     7861 attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct gttccgtggc
     7921 tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa tatcagttat
     7981 caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg cttctggaaa
     8041 gatagtggca ttgcggttga tctgacgtca gcgggaatgc gtgtggagat gggctcattg
     8101 acaacgctgc tgagtggcgg tgtcagcttt gatgtgccgg aaggtctgga tttagggcag
     8161 ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat tcaggattcg
     8221 ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg cggtctgcaa
     8281 ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa agtgccattc
     8341 tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt gctgattcgt
     8401 atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt tgagcacctt
     8461 ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct ggtcactggc
     8521 gcactgtatg ttgatctcga tttctatcca aatacgcctg caataaccgg tattcgtgaa
     8581 tttaatggtt atcagattat cccgaccgtt agcggcggcc tggcgcaaat ccagcaacga
     8641 ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat tgaacaggca
     8701 accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac gctggatagc
     8761 atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat gcagtcaacg
     8821 ttgcgtgaat tgaatcgcag tatgcagggc ttccagcctg gctccgcagc ctacaacaag
     8881 atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc ggtgctgaaa
     8941 acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa agatccagag
     9001 ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg tggctggccg
     9061 gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg cagagcggta
     9121 cacaaagtac cgccagccag ggcaatcgcc tgttatgggt agagcaggtc gctgtacctg
     9181 actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat gtgattgcca
     9241 acaacaactt gtgggccagc ccgttggatc aacagttgcg caataccctg gtagccaacc
     9301 tgagcacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc gcccaggaca
     9361 cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc attgtcagtg
     9421 gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt ctggaaggag
     9481 tgcaaactca ggatggttac gatgagatgg ttaaagtgct ggccggtgtc tggagtcagg
     9541 aagccgcttc tattgcacaa gagataaagc gtctacctta attataaaga tttgtaaata
     9601 taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc ggaaaaattt
     9661 gttcctcttc acattttttg tacaaccgac atgcccgtgt agctcacaaa tatgacagtg
     9721 gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac tgtgattgca
     9781 catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa cgaggcatga
     9841 agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag gccggcatcg
     9901 ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca caatggctgg
     9961 gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt ctctttaaaa
    10021 agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta taatcagaag
    10081 gcagacgtat cctggaacag accgactttc aggtcgctgg cggtatagat cagacgacca
    10141 tcaaccagca cttcgccatc tgccagaccc ataatcagac gacgattaac aatgcgttta
    10201 aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt gaatttcact
    10261 tcgccaacgc ccagcgcgcg gcctttacct tcgccgccca gccagccgag gtagaaccct
    10321 accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc gccaataaag
    10381 tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac ataccctttg
    10441 tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag catgttcggt
    10501 gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc aagaaggtct
    10561 tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat tttattgaag
    10621 cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga ataaaccagt
    10681 tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat gcttgcgcga
    10741 tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac accagattta
    10801 ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat ttgccttctt
    10861 ctaccgcttt caccagttca ctgtgaagac ttaaatggcg aacgttagca gtggggataa
    10921 tgacaccttg tttacccgtt aactcacgtt gctggcaaat agcaaagaag ccttcgattt
    10981 tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa cctgtgatag
    11041 cgatactctg attcaccggc acatcagcga gggcgcttat cagggcgcag agttcagcca
    11101 tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc gatgctgaga
    11161 aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcctgcatg atcatcatcc
    11221 ctttcgcatg gatattgccg ccaagctccg ctttgcgctc gatgtcggtg aattcaccat
    11281 cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt ggatgacccg
    11341 gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg gtttcaatca
    11401 ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct tcgcgccatt
    11461 cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca tcacacaggg
    11521 aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt gtttcttgtt
    11581 caccggtgta gcgtactgct tcgcggataa gtaccggcca ggcatccgct cccggtgcag
    11641 gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactggctt actgactccg
    11701 catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct gaaagctctg
    11761 gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg actttcagct
    11821 tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac gcaacccagt
    11881 caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt tgcgccagca
    11941 gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga ccaggctgca
    12001 gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct tctacccagt
    12061 cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact gcgttacgta
    12121 agcggatggt gtggccggaa acctcatagt gaccgcccac gagttggcct gcatcgcttt
    12181 gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc ggggccttcg
    12241 ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca aattgcagcc
    12301 gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc tgagcaaata
    12361 tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc gtaatggtca
    12421 aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg gcactacagc
    12481 acacgtagaa agcaccgaca atactcctgg catgggcgtt aaagctcaca ggatggagat
    12541 tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg taacattgag
    12601 atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg gaagtatctg
    12661 gtgaagaagc atcgcgaagg ggagttaatt acccgttaca tagaagccag tgccgcccag
    12721 gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa tggctggatt
    12781 gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg ggccagacgg
    12841 aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga tctggaattt
    12901 attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct ttctgaaacg
    12961 attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa aatgtcttct
    13021 ttgaagcatg atctgcaggc attgctgggt aaggaataac tgacgaaact cagttcaatt
    13081 tactaaaggt gaaaaaaaac cccgcagcag cggggttttt taccagacga gaacttaagc
    13141 ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctcaacgc gacgatccgg
    13201 agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt tgccagtaac
    13261 cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac ctttagagat
    13321 caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt aagcgtcaga
    13381 accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat ccaggttgct
    13441 cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg ttgctttgtt
    13501 gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt ccggtgccgg
    13561 agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt aggaaacacc
    13621 caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac cgatgttgtt
    13681 ggtccactga tattccagac gggtagcgat ttcaggagtg atcgcgtact caacaccgcc
    13741 agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg atttggtgtc
    13801 tgcacgccaa accataccac ccagacgagt gtacacgtcc aggtcgtcag tgattgggta
    13861 acccagttta gcggtcaact gaacgccctg agctttgtat gcaccgtttt caacgctgcc
    13921 tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat acgggttaac
    13981 ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc cattgttgtc
    14041 gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt accaggtgtt
    14101 atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg caatcgcgat
    14161 agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg aatatctcca
    14221 acgagataac acggttaaat ccttcaccgg gggatctgct caatattaac tctaccgata
    14281 tcttcggctt atgccgagca cccctggcga tgtaaagtct acaacgtagt tgaaaactta
    14341 caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg tataaggtac gtttaatctt
    14401 ttttgtcagc gacaatttac agaagaaaat cgcggaaacc gcttcagaca agcctccgca
    14461 aggaaaatta gtcacgactg aaagcattgg ctgggcgaca aaaaaagttc caggattaat
    14521 cctaaattta cttaatgata caaattagag tgaattttta gcccggaaag ttgtctcgtt
    14581 gcgtgagagg atgcgcttac cggacgcata ataaacccca tagcgttacc ttcatttgcc
    14641 gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat ctgccaacca accgatcacc
    14701 acactgtaat tgcccgtgcg taaagcgcga accattgact ccacagtgtg gcaagggggg
    14761 agctggctaa tctgcattac tttcgttaag ggtagcccag atgcctgaac ccattcccga
    14821 ctcagttttt gttgcggtgt taaccagagt tgccagcgcg attgctgacc gagttgctgt
    14881 aacaatggca acagtagaag ttgcgtcatc atgggctgat cttcgcgata gacaacttca
    14941 ctgataagcc cggctgtagt gttttccgta gagacacgcg caattttact tgctgcggat
    15001 gagaacgacg aagaacgatg tgcatagcct gaagtgtaca taatcaatcc agcccctgtg
    15061 agttactgta tggatgtaca gtacatccag tgacaacaaa gatcaaccct attttcggaa
    15121 agagcctcgc aaattttgtc gttggtgacg ggaaaacata aattaatctt gcccctaaag
    15181 aataagttgc ctattttcgt atttaacgga tccgttaatg tgaatcattc ttttatgtta
    15241 tgattttaaa aggaatttta tgaaaagcct ctcctataag cggatctata aatcacaaga
    15301 atacctggca acgttgggca caattgaata ccgatcattg tttggtagtt acagcctgac
    15361 cgttgacgac acggtatttg cgatggtttc taatggtgag ttgtatcttc gggcttgtga
    15421 gcaaagtgca cagtactgtg taaaacatcc gcctgtctgg ctgacatata aaaagtgtgg
    15481 ccgatccgtt accctcaatt actatcgggt tgatgaaagt ctatggcgaa atcaactgaa
    15541 gctggtgcgt ctgtcgaaat attctctcga tgcagcgctg aaagagaaaa gcacgcgcaa
    15601 tacccgggaa agactgaaag atttgcccaa tatgtctttt catctggaag cgattctcgg
    15661 ggaggtgggg attaaggatg tacgggcgtt acgtatactt ggggcaaaaa tgtgttggtt
    15721 gcgactgcgg cagcaaaaca gtctggtgac agaaaagatt ctgtttatgc ttgaaggtgc
    15781 cattatcggc attcatgaag ctgcgctccc ggtggcacgc cgccaggagc ttgcagaatg
    15841 ggctgactct cttacgccga aacaggagtt tcctgcggaa cttgagtaat ctggcgttgc
    15901 agacgaccaa tctcaggcag taatgcaatc aataacccaa cttgttggac gaccagaggt
    15961 tctttgctgt ctgcccgtgg ttcaagttgc tgcatccgct gtttcaggcc agctaatgcc
    16021 tgattgacgc gttcttcatc agcaggttga tgatgtaacg cgtcatcaac atagcaaact
    16081 gcgtcatcaa gaaacgccag aatttcagga ttagttaact gctcccggtg agcaccgagg
    16141 gctgagatat agctggtaaa cgtatggtta aggcacagca accgaaacgc ggcttcgcga
    16201 atttgcgggg taacgttcgg ttcgctggac atatttgata ccaccgacgc cagttcagca
    16261 tcacggttgt gtgcatcgcg gcgggcaata cgatacgcca gacggttatc acgcccctga
    16321 tggtattgct ccagtatggc atcgagatag cgacagttgg cctctgtggc gcgttcgagc
    16381 atgcgcggca gattgcgaaa ctgccagtca ggccagatgt agctcactgc cgcccacgca
    16441 atggcacaac caatcagcgt atcgattacg cgaggtaacg ctacttcaaa accttcaccc
    16501 agtaagttaa aacacagtag caccaaaagt gtgatgaaca tcgttgcatg agcgtattgc
    16561 acgttacgga aggcaaaaaa gagcacgccg gtaataacca gcagcaccag ctgcccttcc
    16621 agtgatggca caaaccacag cacaggaatg ccaatggcga tacctaccag cgtaccaata
    16681 atccttaact tcaggcggtg gcgcgtggcg ttatagtttg gctggcagac aaacaaactt
    16741 gtcagcaaga tccaataccc gtgatgcatt ccggttattt gaatgatggc gtagccgaag
    16801 cacaacacca gcgacattct taccgcatga cggaagaggg cggattccgg cgtgaagtga
    16861 cggctaagac gcagccagat atcactcaat ccgtgcgggc tgtcatcagc gagctcattt
    16921 tcgtcattat tatggggcag tgcctgggcc tgttctgatt caattgttgc cagttgggca
    16981 tcaatggcgc gtaaattgtt cagcaaaaat cccagtgttt tgagtaaatc ggcaggcgcg
    17041 ccgttatcgc gcatccgctc cagggcagca tcaatatgcg taaaagcgcg ctcaaaatgc
    17101 ggatcatgtt gataaggctg acgcagcaaa atacagcgtg acagttgctg gcacgcctgg
    17161 ccctgcatcg acatcagccg ctgaaaacgg aacagcacgt cgctgtggcg aaaatgttca
    17221 cgcaatgttt gatactgaat atgagaagag ctggcacgct cgtgaatatc ctgtgcgaca
    17281 aagtaataat gcagcgtgcg acgcgttccc cgttgaccac gatcgccacg taagcgggtc
    17341 agcagcgaga gtttcgtctg attcaatgtc gccatcagct gaccgttggc gagagccaaa
    17401 tcgtacagcg gtgcctggct ttcatcttca atatcaggat caaacatgcg cgacttgagc
    17461 tcaagataac gcgccagttg ttcatagcaa cgcgccaggt tgtcctgcag cgggcggacc
    17521 gggaacagca gatgaccaat aagtgtcagg acgttgtacc agacggcacc ggccagcaga
    17581 tacatcggct gctgatacca gtgctcatac agtgatgttc ccaacattgt gtaaatggcg
    17641 atcagcaatg caccgaaggc aattgttgca tagcgttgac ccagaccgcc gagcaaaatg
    17701 aagccgctgg tagagagcgt taagccaatc gcaaatagcc agggccaggg aaacagcaat
    17761 tctactgagg ccgaggcgat aaaaaagcag aacagcgtaa tgatgaggtt acgcaaacgt
    17821 cccgccagtc ggtcatcgag atcggtcagc gccgctgcca ccatccccag cgtcagcgga
    17881 atcgtcagtt ttacatcacc cagccaccac ggaaacgctg tggttccaca aagcgcaata
    17941 aaaatacgcg cgtaatacag ccaggcgctg ttccaggtat agcgtttgag caaaggactt
    18001 agcatatagg ccatattagt ggattattca aaacgacgac gtgcattggc ttcacgcgca
    18061 gcttgcgctg tttctaccga taccacgcga cgaccaaccg gccatagtgc aatagcggca
    18121 attttaaagt tcgcaatacc gacaggaatg ccaatgatgg aaatacattg tgcgatgccc
    18181 gttgcaatgt gcatcaggca taaccaccag ccaaagaaaa tcagccagaa aatattcaat
    18241 accgtaccgc cagtattcag cagcacattt ttgccagccg ggttcagttc atcgacatga
    18301 atagcttcat tgccataagg caccagagac agtttagtga tctcccagca ggatcgtgtc
    18361 agcggtaagg taaaaatcag cacaatactg accagagtcg ccaacagcca gcccagagtg
    18421 gtggcaaatc cgccaagcac aaagttcaga atgttcaaaa cggtacgcat aaaacctcgc
    18481 tttactgtgg ttttcagtaa tgggcggcaa ttgtaacgtt tttttgggct ggagcacgtt
    18541 ttctctgacg gttacactga taagaaataa tttcgtgtgg atctacagag tcatggaact
    18601 gaaagcgaca acgcttggaa aacgtctggc acagcaccct tacgatcggg cggtgatcct
    18661 caatgccggg attaaagtct ccggcgatcg ccacgaatac cttattcctt tcaatcaatt
    18721 actggcgatt cactgtaagc gcggtctggt atggggcgag ctggaatttg tactgccgga
    18781 cgaaaaagtg gtgcgtctgc acggtaccga atggggcgag acgcagcgtt tttaccatca
    18841 tcttgatgct cactggcggc ggtggagtgg cgagatgagc gaaattgcgt ctggtgtttt
    18901 acgccagcaa ctggatttga ttgccacgcg cactggagaa aataaatggc tgacgcgtga
    18961 gcaaacctct ggcgttcagc aacaaatccg ccaggctttg tcggcgttgc cgttgccggt
    19021 taaccgactg gaagaattcg ataactgccg tgaggcgtgg cgtaaatgtc aggcctggtt
    19081 gaaagatatt gaaagcgctc ggttgcagca taaccaggcg tataccgaag ccatgcttac
    19141 cgagtatgcg gattttttcc gccaggtcga gtcttcaccg ctgaatccgg cgcaggcccg
    19201 ggcagtcgtt aatggcgagc attctctgtt agtgctggca ggtgcaggaa gcggaaaaac
    19261 gtcggtgctg gtggcccgtg caggctggtt gctggcgcgt ggtgaagcgt cccctgagca
    19321 aattttattg ctggcgtttg gtcgcaaagc cgctgaagag atggacgagc gtattcgcga
    19381 acggctacat accgaagaca ttaccgcacg cacgtttcat gcgctggcgc tgcatattat
    19441 tcagcagggc agcaaaaaag ttccgatagt cagcaaactg gaaaatgata ccgctgcccg
    19501 tcatgaactc tttattgctg agtggcgcaa gcaatgcagc gaaaagaaag cgcaggcgaa
    19561 gggctggcgg caatggctga cggaagaaat gcagtggtca gtgccagaag gtaacttctg
    19621 ggatgatgaa aaattacagc gtcgtcttgc ctcacgcctc gatcgttggg taagtctgat
    19681 gcggatgcac ggtggtgcac aggcagaaat gattgccagt gcacccgaag agattcgcgg
    19741 tctgttcagt aaacgtatca agttgatggc cccgttatta aaagcctgga aaggtgcgct
    19801 gaaggcagaa aacgctgtcg atttttcggg ccttattcat caggcgattg tgattctgga
    19861 gaaaggtcgc tttatcagcc cgtggaagca tattctggtt gatgaatttc aggatatctc
    19921 gccgcagcgg gcagcgttgt tagcggcatt acgcaagcaa aacagtcaga cgacgttgtt
    19981 cgccgttggt gatgactggc aggcgattta ccgattcagc ggtgcgcaaa tgtcgctcac
    20041 caccgctttc catgaaaact ttggtgaagg cgaacgctgt gatttagaca cgacttaccg
    20101 ttttaacagt cgtatcggtg aggtggcaaa ccggtttatt cagcagaacc caggccagct
    20161 gaaaaagccg ctaaacagct taaccaatgg agacaaaaaa gccgtcacgt tattggatga
    20221 gagtcaactt gacgctttgc tggataagct ctctggttat gccaaaccgg aagagcgcat
    20281 tctgatcctg gcgcgttacc atcacatgag gcctgccagc ctggaaaaag cggcaacacg
    20341 ctggccgaag ttgcaaatcg actttatgac cattcatgcc agcaaagggc aacaggcgga
    20401 ttacgtcatc atcgttggct tgcaggaggg aagtgatggt tttccggctg cggcgcggga
    20461 gtcgattatg gaagaggcgc tactgccacc ggttgaggat ttcccggacg ctgaagaacg
    20521 gcggttaatg tacgtggcgc tgacccgggc acgtcatcgg gtatgggcac tgtttaacaa
    20581 agagaatccc tctccctttg tggaaatact gaaaaatctg gatgtgccgg tggcgagaaa
    20641 accgtaagaa acaggtggcg tttgccacct gtgcaatatt acttcagacg gtccgcgaga
    20701 taacgctgat aatcggggat cagaatatcg accgcgtcgt tgaaatgcgg cgactggata
    20761 atgaagtctg ccgttgccac gttggtggcg accggaatgt tccataccgt cgccagacgc
    20821 agcaaggctt tcacgtcagg atcgtgcggc acggcattta gtggatccca gaagaaaatc
    20881 aatacatcaa ttttcccttc tgagatcaat gcgccaacct gctggtcacc ccccattggg
    20941 ccactcaaca tcgcgttgac gttcatgccg gttgcgcggg aaatcaagtt accggtagtg
    21001 cctgttgcat acagtacgtg ttgttccagt aacggttgat gtcgttccac ccagctcatc
    21061 agcatttgtt tgcagtgatc gtgtgctacc agcgcaatat gtttccgcgc aggtaaagtg
    21121 cgagtcgtca gttccataat gtacatccgt agttaacttt cctacagatt actgtaagca
    21181 cttatcgctg caagataaag accgaaaaag cctgcgcaca ggcacaaaaa tctcaggaag
    21241 atggttgttt ttccgcccat tgcaggaaag tatttcgcgt ttgtgggtca gccagtttaa
    21301 accaatactt cagccgttgt tctgtgagca cctgagactg cggtggaata gcatccagct
    21361 cggaaacacc cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat tgcggtagtg
    21421 aagcgcgttt cgcggatttg ttatagcgtt caacctcgac ctcataatca atcgtttttg
    21481 ccgttgaggt aatggcgaga atatccagct ttaccggaat cggcgtcgca tcgccatcca
    21541 acaattcaag gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc tccaggcgag
    21601 gcaggcgaaa attgacctgg ttgatgagct gggtattaaa actgaccacc agcggtgggg
    21661 agatatacag ccgttcttca ctattggaaa gatgaatcgt cttctcaacg cgaaacacta
    21721 actgatgcgg tccattatcc agttcaatgc tgtccgcacc gcgcagcaga gagctggaga
    21781 ccttcttgcc gtcgagtacc agaagatcga catcggttga aagccgcagc gtggtggcaa
    21841 aaacggatac cggcaaacat aatgcaatca aggtggtcac gatgccggtt ttcataggta
    21901 gctcctgtca aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta atcatactta
    21961 ctaacatata gacatatttc ccgcttttgc tctcattcat tcgtattagc tgcatggttg
    22021 gcatgtcggc tttgtcgtac actttgtgaa acagtctgga ggaaataatg aaagaaaccg
    22081 atattgcagg cattttaacg tccactcata ccattgcgct ggtgggggca agcgacaaac
    22141 ccgatcgtcc cagctatcgc gtgatgaaat atctactcga ccaggggtat cacgtcattc
    22201 cggtttcgcc aaaagttgcc ggcaaaacgc tgctaggcca gcaggggtat ggcacgttgg
    22261 cagacgttcc ggaaaaggtc gatatggtcg atgttttccg taattcagaa gcggcgtggg
    22321 gcgtggcaca ggaagctatt gccattggcg cgaaaaccct ctggatgcaa ctgggggtga
    22381 ttaatgagca agcggcagta ctggcacggg atgccgggtt gaatgtggtg atggatcgct
    22441 gcccggctat tgagatccct cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa
    22501 aaccttccgg ggatttgttc agggattagt tacgcagacg cggggcctgg agttgtttgc
    22561 ggatggtctg cgccagctca tccatggatg gctgttcagg atgttcatcc tgcaattcgc
    22621 tacttagctg ggcttcagcc aggtaggtat gaaccggtag gccgttatcg tcctccatca
    22681 ccacgtgata ccacggagcg gcgcgaagct cgtcattcac cgccagctca tcaggcgacg
    22741 gttcagagag cgaataaacc gggtcgatat ccacgaccac tccgagataa cctaacaggg
    22801 aatggcggac ctgctggccg ataccgaatt tgctggcaat catagtcacc tcccgggaaa
    22861 tctttacctt tgatataagt gtaaaattcc acatttcaag ttacatgacg cgacaggcaa
    22921 accctttcag atatagccct tccggatagg tagcgatcac cggatgatcg gccgcctgac
    22981 ggaactgctc tataaattgt acatcacgac cggcatcaat tgcggcatcc gcgatgattt
    23041 tctgaaataa atcgctggtc atcaggccag aacaggagaa agtcaggaga ataccgcctt
    23101 cattcagcaa ctgaatcgcc agcatgttga tatctttata gccacgacac gcgcccatca
    23161 actggctttt attctcaaca aacttcggcg gatccatcac gataacgtca aatttttcac
    23221 cgcgatcgcg ataagtacgc agcaatttaa agacatcatc acggacaaac tcagccttgc
    23281 tcagatccag tttgttcagc tcaacgttct gccgtgcaat atccagcgct tcctgggagg
    23341 tatcaacgct gacaacctgg ctgcaaccgc ccatcagtgc cgatacggcg aaaccaccgg
    23401 tataggagaa acagttcagc acacgtttat tttcaacgta gcggcgggta gccaggcggc
    23461 tgtctcgctg gtccaggtag tagcccgttt tgtgtccgtg ctgtatgtcc accagcagct
    23521 tcatgccatg ttcttcaatc ggcagcaggg caggcggcaa ctcgccggtg acgaggccct
    23581 gggtcagctc catcccttct tttttacgta ccgcaacatc gctgcgatcg taaatcgcac
    23641 attccgggta cagcgtttgc agggcgctaa ttaatgccgc gcgctgatac tctgccccag
    23701 cactcagcag ttgcagcacc agaaaattac cgaaacgatc gatagtaata cccggcaggc
    23761 catcagattc accggcgatt aaacgatagc tgtcgaggcc atctttttgc gccagccagt
    23821 cacgccattt ttgtgcttgt tgcaaacggc gggaaaaaaa agcaatgtcg atagactcag
    23881 acgggtcaaa cgtccagacg cgcgcccgga tttgcgaagc tggcgaataa gcgccgcgtg
    23941 ctaaccattt tccctgatga tcaacaatat cgatggtttc accgaggctg gctttacctt
    24001 ccatacgggc aacggccccg gaaaagaccc acggatggcg acgaagtaat gatttttcgc
    24061 gccctttggc taacactaaa cgtacactca taatttactt ttctgtcgat gcaaagaaat
    24121 gggcgtcatt gtccggagtt ttccatggat ttgcaacgcg tccattaagg ataaggagag
    24181 cagcaatgtc gaaagtctgc ataattgcct gggtttacgg gcgagttcag ggcgtaggat
    24241 ttcgctatac cacgcagtac gaagcgaaaa aactcgggtt aagcgggtac gccaaaaatc
    24301 ttgacgatgg cagcgtggaa gtggttgctt gcggtgaaga agggcaggta gaaaaattaa
    24361 tgcagtggtt aaagagtggc ggcccgcgtt ctgcgcgtgt agaacgggtg ctcagcgagc
    24421 cgcatcatcc ctcgggtgaa ttaaccgatt ttcgtatccg ttaaatacat tttaccggtt
    24481 ttggcaggcc agcaattttg gtggcttgct tagccggacc tttcggaaac agtcggtata
    24541 aatagcggct gttacctttt tcttcgccaa atttattcgc catcgctttt accagcatac
    24601 gaatcgccgg agacgtatta aattccagat agaaatcacg cacaaaacgc accacttccc
    24661 agtgttctgg cgacagcgaa atcccttcgt tctctgcaat caccactgcc agcggctcac
    24721 tccactggct gctttctttg agatagcctt cggtatccgt ttctatctct ttaccttcga
    24781 agatcagcat aattattact accttaatca gactgccggc agtgtaacaa aaaacaaagc
    24841 cccgcataaa gcgaggctat gacagtgtta gcgggtgaga ttaatcgcgg ctggcgaagc
    24901 ccagaatgct cagcaggctg acgaagatgt tgtacagcga aacatacagg ctaaccgtgg
    24961 cacgaatata gttcgtttca ccgccatgaa tgatgttgct ggtttcaaac aaaatagcgc
    25021 cagaggagat caaaatgaag accgcgctga tcgccagatg cagagcaggc agctgcagga
    25081 agatattagc aaccatacca atcagcacca ccacaatacc cgccatcagc ataccgccga
    25141 ggaacgacat atctttgcgg gtggtcagca catatgcaga gcagcagaag aacactaacg
    25201 ccgttccgcc cagtgccata gcgattacgt cacccattcc ggcagacaga taggtgttca
    25261 gaattggtcc gaggatataa cccagaaaac cggtaaaggc gaatgcggag ataatcccgg
    25321 tcggcttatt cgccgtttta taggtcagga acatcaaacc atacataccc accagcgtca
    25381 gaatcagacc cggagatggc agcatcagca ccgtactggc agttgcggta atcgccgaaa
    25441 aggccagcgt caggctcagc agaaaatagg tattacgcag caccttatgg gtgctaagca
    25501 gtgatgtacg gtcatgtgaa gaactaacaa tacgatccat tgagtcactc tctatgacag
    25561 atgtaattaa ttaagcagca taatgataat gcgtaagggc acccagaagt tttacccatc
    25621 tttacgcatt tgatctggaa caggtttaac agcggattat caggtcatta agcaaatata
    25681 acgccctgag aatttcgaca ggcaaaagaa aaaggggtta gcatttagct aaccccttat
    25741 cttatctggc ggaagcgcag agattcgaac tctggaaccc tttcgggtcg ccggttttca
    25801 agaccggtgc cttcaaccgc tcggccacac ttccggaatg acgcgcacta taaacatccc
    25861 gatgcggcgt gtaaacccct aatttgtttg tttgcctgaa aaacagccaa aagtgcattg
    25921 atagtgtgaa ataacagcag attgatcatt tcatcaccat gaattccttc tcttttactc
    25981 gtttagcaac aggctaaaca tcccaccgcc cggcaaaaag aaaaataggt ccatttttat
    26041 cgctaaaaga taaatccaca cagtttgtat tgttttgtgc aaaagtttca ctacgcttta
    26101 ttaacattac tttctggcga cgtgcgccag tgcagaagga tgagctttcg ttttcagcat
    26161 cttacgtgaa gcgatggttt gccttgctac agggacgtcg cttgccgacc atgtgcgccc
    26221 ggtgtcctgc cggtgtcgca aggaggagag acgtgcgata tgaataacga ggaaacattt
    26281 taccaggcca tgcggcgtca gggcgttacc cggcgcagct ttctcaaata ttgtagtctg
    26341 gctgccacgt cgctgggatt aggcgcggga atggcaccaa agattgcctg ggcgctggag
    26401 aataaacccc gcattccagt ggtatggatc cacggtctgg aatgcacctg ctgtaccgaa
    26461 tcttttatcc gctccgctca cccgctggca aaggacgtca tcctttccct gatttccctc
    26521 gattacgacg atactttgat ggctgccgcc ggaacccagg cggaagaagt ctttgaagac
    26581 atcatcacgc aatacaatgg caaatatatc ctcgcagtag aaggtaatcc gccgctgggc
    26641 gagcagggga tgttctgtat cagcagcgga cgaccgttta ttgagaaact caaacgtgcc
    26701 gctgccggag ccagcgcgat tatcgcctgg ggaacctgcg cgtcctgggg ctgcgtgcag
    26761 gccgcgcgac ccaatccgac acaggcaacg cctatcgaca aagtcatcac cgacaaaccc
    26821 attatcaaag tacctggctg cccgccgatc ccggatgtga tgagcgccat cattacttac
    26881 atggtgacct ttgatcgctt gccagatgtc gacagaatgg gtcgtccact gatgttttat
    26941 ggtcagcgta tccacgataa atgctatcgc cgcgctcact tcgacgccgg agagttcgtc
    27001 cagagttggg atgatgacgc tgcccgcaaa ggttattgcc tgtacaaaat gggctgcaaa
    27061 gggcctacca cctataacgc ctgttcctcc acacgctgga atgatggcgt ttctttccca
    27121 atccagtctg gtcacggctg cctgggctgt gcggaaaatg gtttctggga tcgcggttcg
    27181 ttctacagcc gcgtggtcga tattccacaa atgggtactc attccaccgc cgataccgtc
    27241 ggtttaaccg cgcttggtgt ggtggcagcg gctgttggtg tgcacgcagt cgccagcgcc
    27301 gttgaccagc gcagacgcca taatcagcaa cctacagaaa ccgaacatca gccaggcaat
    27361 gaggataaac aggcatgagc actcagtacg aaactcaggg atacaccatc aataatgccg
    27421 gacgccgcct ggtggtcgac ccgattacgc gcattgaagg ccacatgcgc tgcgaagtga
    27481 atattaacga tcagaatgta atcaccaatg ccgtctcctg cggcaccatg tttcgcgggc
    27541 tggagatcat cctgcaaggg cgcgacccgc gcgatgcgtg ggcgttcgtt gaacgtatct
    27601 gcggcgtctg tactggcgta cacgctctgg cttcggttta cgccatcgaa gatgccatcg
    27661 gtattaaagt gccggacaac gccaatatca tccgcaacat tatgctggca acgctctggt
    27721 gccacgatca tctggtgcac ttctatcagc ttgccgggat ggactggatc gatgtgttag
    27781 atgcgctgaa agccgacccg cggaaaacct ccgaactggc gcaaagtctc tcctcttggc
    27841 cgaaatcatc ccctggctat ttcttcgacg tacaaaatcg cctgaagaaa ttcgttgaag
    27901 gcgggcagtt ggggatcttc cgcaatggct actgggggca cccgcagtac aaactgccgc
    27961 cagaagccaa cctgatgggc tttgcccact atctcgaagc tctcgatttc cagcgtgaaa
    28021 ttgtcaaaat ccacgcggtc tttggcggta aaaacccgca tccaaactgg attgtcggcg
    28081 ggatgccttg cgccattaac attgacgaaa gcggcgcggt cggggcggtc aatatggaac
    28141 gcctgaacct ggtgcagtcg attatcaccc gcacggcaga cttcattaac aacgtgatga
    28201 tccccgacgc cttagccatc ggtcagttca acaaaccgtg gagcgaaatc ggcacgggtc
    28261 tttctgataa atgtgtcctc agctacggcg cattcccgga tattgccaac gactttggtg
    28321 agaaaagtct gctgatgcct ggcggcgcgg tgattaacgg cgacttcaac aatgtgctgc
    28381 cagtggattt gattgatccg cagcaggtgc aggagtttgt cgatcacgcc tggtatcgtt
    28441 atcccaacga tcaggtcggg cgtcatccat tcgatggcat taccgacccg tggtacaacc
    28501 ccggcgatgt caaaggcagc gataccaaca ttcagcagct gaatgaacag gaacgctact
    28561 cgtggatcaa agcgccgcgc tggcgcggta acgcgatgga agtggggccg ctggcacgca
    28621 cgttaatcgc ttatcacaaa ggcgatgctg cgaccgttga gtcggtcgat cgcatgatgt
    28681 cggcgctgaa cctgccgctt tccggtatcc agtcaacgtt gggccgcatt ttgtgccgcg
    28741 cgcacgaagc acagtgggcc gcaggtaagt tgcagtattt cttcaacaag ctgatgacta
    28801 acctgaaaaa cggcaatctt gccaccgcct ccacggaaaa atgggaaccc acaacctggc
    28861 cgaaagagtg ccgtggtgtc ggctttaccg aagcgccgcg cggagcgtta ggccactggg
    28921 ccgccattcg cgatggcaag cttgatctct accagtgcgt ggtgccgacc acctggaacg
    28981 ccagcccgcg cgatcccaaa gggcaaattg gcgcttatga agccgcgctg atgaacacca
    29041 aaatggcgat ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc tttgacccgt
    29101 gcctcgcctg ttcaacgcac gtgctgggcg acgacggaaa cgagcttatc tccgtgcagg
    29161 tacgttaaca gcgaaggaga atcatcatgc aagagaaaag cgacaatgtt gtcagccact
    29221 atgtctttga agcgccggtg cggatctggc actggctgac ggtgttatgc atggcggtat
    29281 tgatggtcac cggttacttt atcggcaagc cgctaccttc cgtcagcggc gaggcgacgt
    29341 atctgttcta tatgggctac atcaggttaa ttcacttcag caccgggatg atttttaccg
    29401 tggtcttgct gatgcggatc tactgggctt ttgttggcaa tcgatactcc cgcgagctgt
    29461 ttatcgtgcc ggtatggcat aaaagctggt ggcagggcgt gtggtatgaa atccgctggt
    29521 atctgtttct ggcaaaacgt ccgagtgccg atataggcca taaccccatc gctcaggcgg
    29581 cgatgttcgg ctatttcctg atgtctgtct ttatgatcat caccggcttt gcgctgtaca
    29641 gcgaacacag ccagtacgct atttttgcgc cgttccgtta tgtggtggaa tttttctact
    29701 ggacgggcgg caactcaatg gacattcaca gctggcatcg gctggggatg tggctgattg
    29761 gcgcgtttgt gatcggtcat gtctatatgg cgctgcgtga agacatcatg tccgacgaca
    29821 cggtgatctc caccatggtc aacggctacc gtagccacaa atttggcaaa ataagtaaca
    29881 aggagcgttc atgagcgagc aacgcgtggt ggtaatgggg ctgggcaacc tgctgtgggc
    29941 cgatgaaggt ttcggcgtgc gggtggcgga acggttgtat gcccattacc actggcccga
    30001 gtatgtggag attgtcgatg gcggtactca gggactgaac ttgctggggt atgtcgaaag
    30061 cgccagccat ctgttgattc tcgatgccat tgactacggg ctggaacctg gaacgctgcg
    30121 aacctatgcc ggagaacgta ttccggctta tctcagcgcg aagaaaatga gcctgcacca
    30181 gaacagtttc tccgaagtgc tggcgctggc agatatccgc ggacatctgc cagcacatat
    30241 tgccctcgtc ggtctgcaac ccgcaatgct cgacgactac ggcggcagcc tgagcgaact
    30301 ggcacgggag caactgcccg cagcggaaca ggcggcgctg gcgcagctgg ctgcatgggg
    30361 aattgtgccg caaccggcta atgaatcgcg ctgtctcaat tatgactgtc tgtcgatgga
    30421 aaattacgaa ggcgttcgct tgcgccagta caggatgaca ctggaggagc agggatgagc
    30481 aacgacacgc catttgatgc gttgtggcaa cgaatgctgg cgcgcggctg gacgccggtc
    30541 agtgaatccc gtcttgacga ctggcttacg caagcgccag acggcgtggt gttattaagc
    30601 agtgacccga aacgcacgcc agaggtcagc gataatccgg taatgattgg cgaattactg
    30661 cgcgagtttc ccgactatac atggcaggtg gcgattgctg accttgagca gagcgaagcc
    30721 atcggcgatc gctttggcgt ctttcgcttt cccgccactt tagtgtttac cggcggaaac
    30781 tatcgcggcg tgctgaatgg tattcacccg tgggcggaac tgataaacct gatgcgtggg
    30841 cttgtcgaac cacaacagga gcgtgcctca tgagcgaaac ttttttccat ctgctggggc
    30901 caggaacgca accgaacgat gacagtttca gcatgaatcc actgccgatc acctgtcagg
    30961 tgaatgatga accgagtatg gcggccctgg agcaatgtgc tcacagcccg caggtgaatg
    31021 cgctgttaaa cgagttacaa catcaactaa gtcaacgcca gccgccgttg ggcgaggtgc
    31081 tggcagtcga tctgttaaat ctcaacgccg acgatcgtca ctttatcaat acgcttctcg
    31141 gggaagggga agtgtcagtg cgcattcagc aggctgacga cagtgaaagt gaaatacagg
    31201 aggcgatctt ctgcggatta tggcgggtgc gcagacgtcg cggcgaaaag ttgctggagg
    31261 acaaactgga agctggctgc gcgccgctgg cgttgtggca ggcggcaacg caaaatctct
    31321 tgccgacaga ttcgctgtta ccgccgccca ttgatggcct gatgaatggc ctaccgttgg
    31381 cgcatgagtt actggcgcat gtacgtaacc ccgacgcgca gccgcacagc attaatctga
    31441 cgcaattacc catcagcgag gctgatcggc tttttctctc acgtctctgt gggccgggaa
    31501 atattcagat tcgtaccatt ggctatggcg agagctatat caacgccacg gggttacgcc
    31561 atgtctggca tttacgctgt acggacacct taaaaggccc gttactggaa agttatgaaa
    31621 tctgcccaat accggaagtg gtgctggcag cgccagaaga tttggtcgac tctgcgcagc
    31681 ggcttagcga ggtatgtcag tggctggcgg aagctgcacc gacgtaaaaa gacggtaagt
    31741 atcgctttca ggcttatgaa tatcgcaatc ggcgaatacc tctggtcgta gagtttcagg
    31801 ataaagaggg agatctacca ttaacgggtt atttttctct cttcgcctac aggagtgcgc
    31861 atgtgggatg tcattgattt atcgcgctgg cagtttgcgc tgaccgcgct gtatcacttt
    31921 ttatttgtac cccttaccct ggggctgatt tttttgctgg ctatcatgga aaccatttac
    31981 gtggtcaccg gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa gctcttcggt
    32041 atcaactttg ctcttggcgt ggctaccggc ctgaccatgg agtttcagtt tggtactaac
    32101 tggtcattct attccaacta tgtgggcgat atttttggcg caccgctggc gatggaagca
    32161 ttaatggcct tcttcctcga atccaccttt gtcgggctgt tcttcttcgg ctggcagcgg
    32221 ctgaataaat accagcacct gctggtgacg tggctgatgg cgttcggttc aaatctctct
    32281 gcattgtgga tattgaatgc caacggttgg atgcaatacc cgaccggtgc gcattttgat
    32341 atcgacaccc tgcgtatgga gatgaccagc ttcagcgagc tggtctttaa cccggtcagc
    32401 caggtgaaat ttgtgcacac cgtaatggcg ggctacgtga ccggggccat gtttattatg
    32461 gcgatcagcg cctggtattt actgcgcgga cgggagcgcg atgtcgcatt acgctcgtct
    32521 gccatcggtt ccgtcttcgg tactctggcg attattggca cgctgcaact cggagacagt
    32581 tctgcgtatg aagtcgcgca agtacaaccg gtaaaactgg cggcgatgga aggggagtgg
    32641 caaacggaac ctgcacctgc accgttccat gtggttgcct ggccggaaca ggatcaagag
    32701 cgtaacgcct ttgccatcaa aattcccgcg ctgctaggga tcctcgccac tcactcatta
    32761 gataaacccg tgccgggtct gaagaatttg atggctgaaa cctatccacg cttgcaacgc
    32821 ggacgtatgg cctggctgtt aatgcaggaa atatcgcaag gcaatcgtga gccgcatgtg
    32881 ttgcaggcat tccgggagct ggaaggcgac ctgggctacg gcatgttgct ctcccgctat
    32941 gcgccggata tgaatcatgt cacagccgca cagtaccagg cggcgatgcg tggtgcaata
    33001 cctcaggttg cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg ttccctgctg
    33061 ctactggtga tgctgattgc gcttgtccag acgctgcgtg gcaaaatcga ccagcatcgc
    33121 tgggtgctga aaatggcgct ctggagtttg ccgttgccgt ggattgcgat tgaagccggg
    33181 tggtttatga ccgagtttgg tcgtcagccg tgggcgatac aggacatctt accgacatac
    33241 tccgcgcact ccgctttaac cacaggacaa ctggctttct cactgatcat gatcgtaggc
    33301 ctttacaccc tgttcttaat cgccgaagtc tacctaatgc agaaatatgc ccgtctgggg
    33361 ccgagcgcga tgcagagtga acaaccgacg cagcaacagg ggtaaaggag aaaatcatgt
    33421 ttgattatga aacattgcgc ttcatctggt ggctgctgat tggcgtgatc ctggtggtct
    33481 ttatgatctc cgacggattt gacatgggga ttggctgttt gctgccgctg gtggcgcgtg
    33541 atgatgatga acgccggata gtgattaata gcgttggtgc gcactgggaa ggcaatcagg
    33601 tctggttgat cctcgcaggt ggggcattat ttgccgcctg gcccagagtg tatgcagcgg
    33661 cgttttccgg cttttatgtg gcgatgatcc tggtgctgtg ctcgctgttc ttccgcccgc
    33721 tggcctttga ttatcgcgga aaaatcgccg atgcacgctg gcgtaaaatg tgggatgccg
    33781 gtctggtcat cggcagtctg gtgccgccgg tagtcttcgg tatcgccttc ggcaacttgt
    33841 tgctcggcgt gccgtttgcc ttcacaccgc aattacgcgt ggagtatctc ggcagcttct
    33901 ggcaactgct gacgccattc cctttattgt gcggattgct cagccttggg atggtgattt
    33961 tgcaaggtgg cgtctggtta caactgaaaa ctgttggtgt gattcatctg cgttcacagc
    34021 tggcgactaa acgcgctgca ctgttggtga tgctgtgctt tttgctggcg ggttactggc
    34081 tgtgggtcgg tattgatggc tttgtactgc tcgcccagga tgctaacggt ccttccaatc
    34141 cgttaatgaa actggtggca gtgctacctg gtgcctggat gaataatttt gtcgagtcgc
    34201 ccgttttgtg gatcttcccg ctgctggggt tcttctgccc attgctgacg gtgatggcga
    34261 tttatcgtgg tcgcccgggt tggggatttt tgatggcgtc attgatgcaa tttggcgtga
    34321 ttttcacggc aggcatcacg ctgttcccct ttgtcatgcc gtcaagcgtg agtccaatct
    34381 ccagcctgac gttgtgggac agcacttcca gtcagctgac gctgagcatt atgttggtaa
    34441 tcgtgctgat atttttgccc attgtgttgc tctacactct ctggagctac tacaaaatgt
    34501 ggggacgcat gacgacagaa actctccgcc gtaacgaaaa cgagctgtac taaggagcag
    34561 gaacaatgtg gtatttactt tggttcgtcg gcattttgtt gatgtgttcg ctctccaccc
    34621 ttgtgttggt atggctggac ccgcgattga aaagttaacg aacgtaagtc tgatgcggcg
    34681 cattagcgtc gcatcaggca atccataatg tcggatatga aaagcggaaa catatcgatg
    34741 aaagcgatct tgatcccatt tttatctctt ctgattccgt taaccccgca atctacattc
    34801 gctcagagtg agccggaact gaagctggaa agtgtggtga ttgtcagtcg tcatggtgtg
    34861 cgtgcaccaa ccaaggccac gcaactgatg caggatgtca ccccagacgc atggccaacc
    34921 tggccggtaa aactgggttg gctgacaccg cgcggtggtg agctaatcgc ctatctcgga
    34981 cattaccaac gccagcgtct ggtagccgac ggattgctgg cgaaaaaggg ctgcccgcag
    35041 tctggtcagg tcgcgattat tgctgatgtc gacgagcgta cccgtaaaac aggcgaagcc
    35101 ttcgccgccg ggctggcacc tgactgtgca ataaccgtac atacccaggc agatacgtcc
    35161 agtcccgatc cgttatttaa tcctctaaaa actggcgttt gccaactgga taacgcgaac
    35221 gtgactgacg cgatcctcag cagggcagga gggtcaattg ctgactttac cgggcatcgg
    35281 caaacggcgt ttcgcgaact ggaacgggtg cttaatttcc cgcaatcaaa cttgtgcctt
    35341 aaccgtgaga aacaggacga aagctgttca ttaacgcagg cattaccatc ggaactcaag
    35401 gtgagcgccg acaatgtctc attaaccggt gcggtaagcc tcgcatcaat gctgacggaa
    35461 atatttctcc tgcaacaagc acagggaatg ccggagccgg ggtggggaag gatcactgat
    35521 tcacaccagt ggaacacgtt gctaagtttg cataacgcgc aattttattt actacaacgc
    35581 acgccagagg ttgcccgcag ccgcgccacc ccgttgttgg atttgatcat gacagcgttg
    35641 acgccccatc caccgcaaaa acaggcgtat ggtgtgacat tacccacttc agtgctgttt
    35701 atcgccggac acgatactaa tctggcaaat ctcggcggcg cactggagct caactggacg
    35761 cttccaggtc agccggataa cacgccgcca ggtggtgaac tggtgtttga acgctggcgt
    35821 cggctaagcg ataacagcca gtggattcag gtttcgctgg tcttccagac tttacagcag
    35881 atgcgtgata aaacgccgct gtcattaaat acgccgcccg gagaggtgaa actgaccctg
    35941 gcaggatgtg aagagcgaaa tgcgcagggc atgtgttcgt tggccggttt tgcgcaaatc
    36001 gtgaatgaag cacgcatacc ggcgtgcagt ttgtaatgca taaaaaagag cattcagcta
    36061 cctgaatgct ctgaggctga tgacaaacga agaactgtct aatgcgtaga ccggaaaagg
    36121 cgttcacgcc gcatccggtc actttacctt cagttttact ctttctcgga gtaactataa
    36181 ccgtaatagt tatagccgta actgtaagcg gtgctggcgc gtttaatcac accattgagg
    36241 attgcgcctt taatattgac gcctgcctgt tccagacgct gcattgacaa gctcacctct
    36301 ttggcggtgt tcaggccaaa acgcgcaacc agcaggctgg tgccgacaga gcgccccacg
    36361 accgcagcat cgctcaccgc cagcatcggc ggcgtatcga caatcaccag atcgtaatgg
    36421 tcgttcgccc attccagtaa ttgacgcatc cgatcgcgca tcagcagttc agacgggtta
    36481 ggtggcacct gaccgcgagt aatcacatca aagcctcctt tgccaaaatg ctggatcact
    36541 ttgttgagct catctttacc tgccagatat tccgacaagc catgttcatt actcacggta
    36601 aacaggttat gcgaataacc acggcgtaag tcggcatcaa taaataacac tttttgatcg
    36661 gactgggcga tcaccgctgc cagagttgaa ctgacaaatg ttttaccact gtctggcgtc
    36721 gcaccggtga tcatcagaat gttattctca gtctccatca tagcgaaatg cagactggtt
    36781 cgtagcgcac gtacggcttc cacagcagaa tccgccgggt tatccaccgc caggaagggg
    36841 atatttttag tacgatggcg ctgctgatta gaaaataaat ttttcttacg cagacgggta
    36901 cgtttatcca gccactcgga catcgggatg gtggcataaa cgctgatgcc gtgctcttcc
    36961 agttgttccg gggcttctac accacgacgc aacatcgcac gcgccagcac ggcacccacc
    37021 gaaataaaca ggccaagaat aaaaccaagc accacattca acgctttttt cggtttcact
    37081 ggctgcggct gagtgactgc cgggtcgata atccgcacgt taccaatggc actggatttc
    37141 gaaatactca actcttgctg gcggttaagt aattgcagat ataccgcacg gcccgcttct
    37201 acgtcacgac ttaaacgcaa cacttcctgt tgggtggaag gcatagccga tacccgctta
    37261 ttcaggcgtt tgcgttcttg ctccagcgtc tggcgttttt ccagcagcgc acgataagtt
    37321 gggtgatctt tcttatacag ctgggagatc tctgcctcgc ggaaggtcag ctcattgagt
    37381 tgattatcaa cgttcacaat ctgctcaaga acggctttgg cttccaggtt aaggtcaacc
    37441 gaatcgcgct gctggcgata aacgttgagt ttttcttccg cctggtccag ctcgctgcgc
    37501 acttcaggca actggcgctg taagaattca aggctttgtg aatcctgcgc cgcctggcga
    37561 gcgatattct gttgcaaata gttgttagcg atgctgttca gtatacgggt aatcaactgg
    37621 ggatcatcac cggtcatggt aagttccagc atcccgcttt ctttactgcg ttcgctaacg
    37681 gtaaaggttt cctgcaacgc gttaatcgct tccagttcgg tacgttggct caggacaaac
    37741 tgtgttcctg gtttggcctt aatatccgcg atagtcagcg caacgccatc tttttccaga
    37801 cgctggccga ccataccatt gacggtgaac tcttcacctt ccagcgtata gtggccgttt
    37861 tccccaaccg tgagtgtcag ttgctgatcc tgaccattca gttgtggaat atgcatccag
    37921 ctgatcgcca gctcacctgg tttctctttg gttaatctcg cccagccgcg acccacaatc
    37981 ggaaaatact tctgctcaac catgtcgcgc agattcagtt cagcaatggt tttaccgaga
    38041 atcatgcgcg attgcagcag ttggatctcc ggtgcagact cgggcgatga gttagggatc
    38101 atatcactca ggccgctgag aatggcgttg ccctgttttt gctcaacctg gaccagagta
    38161 tctgcctgat aaattggtgt acttaacagc gagtaagcga cagcgatcag cgtgaataac
    38221 gcggtcacgc tgataataaa cttacggtga tcccataact cgccgaccag acgaagcaga
    38281 tcgatctcat tttcctgagt gctgcctggt ggcgtattca tatttttagt tgtcatcgta
    38341 ttttataaac tcaaattacc ggctgaggcg cttcgcccat tcctgactgg cgcgctccaa
    38401 cataccgtag acatgttcaa atgcgtcctg acttttacga tagggatccg ggatctcttt
    38461 ctgttccagc cattgcccaa acagcattgt cttcccgcgg acctcgggag cgattgcggt
    38521 aacctgagca atatgttccg actccattgc cagaatcaga tcgtaatttc gtgccatctc
    38581 agcagtgagc ttacgtccgg catgtccttc aagagatacg ccatgattag cggcaacatc
    38641 tgctgctgtc gcatctgcag ggtgttttac cagaccatga acacctgccg atttgacttt
    38701 tacgcctggc agacgtttac gcagcaagcg ctcgccaatg ggcgaacggc aaatgttgcc
    38761 ggtacaaacc accaggattg agttaaattt tagttgggcc atgtttgaat ccaccgtgaa
    38821 gtctctgtca ggtcatgcac cccggaaatc gtcggtacca gcagggaaat cacacggttc
    38881 caacgagcca gcggcgcggt agtgacataa acaatgtcgt aaggttcaag ctggaattcc
    38941 gttccgagta tcattgccga tgcgtccttc gcattcagct gataaatatt ggcgattttg
    39001 ccattctgct tgttttgcgt tgcacgaata acaaaaatcc ccgtcgcatc agcgacatcc
    39061 tgattcatac cctcagcgtt acccagcgct tccgccaggg tcataccgct gcggtccatt
    39121 ttcagcgtgc tctgtttacc cacttcaccc atcacgaaga ctttcagatc atcgttgcgc
    39181 ggaataaata aaatatcgcc cgggtgcagc agtttgttct ggcgcaagtc gccacgctgc
    39241 atcagcgcat agaggttcac tttggtcttt acgccattct gggtgagcac cacgttgcgc
    39301 cagtctgcat ctgcggtcag accgcccgcg gcgttaatcg catccatgat ggtcagagga
    39361 atattggtaa tcggctgctg acccgatttg ctgacttcgc cggtgacgta cgctttttgc
    39421 gagcggaacg ccgcgacgct gacatcgact tgcgggcttt caatgactga atccagacgc
    39481 gctgtaatct cgttgcgtac ctgggtaagt gttttcccgg ccactttcag gcgaccaata
    39541 taggggtaga aaatcgcacc atcggcgtta acccagttgc cggtatcact ggcgctacgg
    39601 tattgccctg cgggggtggt tagttccggg tgatcccaga ccgtaaccat caacacatca
    39661 ccaatgccga tacgatattc atagttcgcg atttgctgct ccagctccgg gttagcctgc
    39721 gacataatcg gtttggcgcg caactgatca atcaatcctg gcgtgacggg atagatattg
    39781 accattttgt ccaggtcata ctgattgtca ggtagttcaa tgacatcctt gttactggtc
    39841 gataaattct gaccaggaac aagggtacat gctgtcaaag acagaacagc caatgtcagt
    39901 accgaaaact taaaaatgtt ctttttcatt ggtttatatt tatcagtcaa tgtaatgtta
    39961 tgaattacgt caggttattg tccaaccggt acttctcgct ctgccgtcat tggatagagc
    40021 tggtatttgc gtgacagcat ttgtccacca tcacgcgtca atggtgtcca cgaaaccacc
    40081 gcgcggttgc ggttaggtcc aatggtcatc aagtcgaacg gaatcgagat ataaaaacct
    40141 ttactaaagc cgccttcgcc gtagtcatct ttagatacgt tactgattgc cgcccatacc
    40201 cctaccgcca cgccgctgtc aaagcgtttg gcgacgtcga tcgttgcccc tttatctttt
    40261 gccagatatt gcccaacgct aagtttcatc agtacgccat tgagcgtcgg cgggttccag
    40321 taagccgtca cgaaacctgt gggcgtggaa taatcggtga agcgcatcat gttgtcccag
    40381 tcacgctgct tcacgtagtt aacgtccaca cccagcgccc agctggcatc tagcgggcga
    40441 taaagcagct cggaaccgac accggcatac atcgtttcca gataaccgcc atacacctgg
    40501 ccatagaaac cattgcctaa gtcggcaaag taattcgcct gcaagttgtt gagataaacg
    40561 tcattgcgaa cgtaatcacg gatatgcgtg cgcacgcggg gcagggtaga gtccgcgggc
    40621 aacagtgaag acttaaactt gtcgtagttg ttgtaaatat tggtgaaaat accgccatca
    40681 agcagcaggt ggtcggtaaa ccagtagcgg gcgctggaca tcagccccag ctggaacata
    40741 tagaaatctt ccgggccgcc cagcgactgt gaaagtgttg ggttgaaaga gtagctaaag
    40801 cgatcttcac gaatacggta gccccgacca aaggcagaaa gatcttcagc ttcaacacgt
    40861 tgttgttgca gtggctctga ttgaccaggc gctgtacctg ccagctgttt gcgcaggctg
    40921 gctacgtcgg tttcggtagt caccatcgcc atatgctcgc gcttttgcgt cacgctaatc
    40981 ttctcaacgc cttgcggcag gttattcacc agaatccggt tggcacgatc gaccgcttca
    41041 cgagagtcac ggtatttgta ttgctgacca gacatataca gcgtcttatc gcgtagctga
    41101 atttctggcg cgtcaaagcc cgcgttatac ttcagggcgg taagttggtt tgctaccgtg
    41161 gtgtactgca atccttcaga ttcaggcgca ggttgatatg ccggttttgg cgtatcgcgc
    41221 agggcagggc gcagatcgtt gaaattggtc cgtaaagtga aaccaaacat caacgtgtta
    41281 ccgcgttcat aactcaggtt gaggtctgcc cagctggcag cgcgataaac tgcaccgacg
    41341 ttgaaatggc ttgcctgagg cagtttgcca gcgaaatcat tctggtaatt gttgccgtcg
    41401 tattcgagtt tcagacgcag gggattccac ggcgtttgat actcaatgcc gccaaagatg
    41461 gaagccgggc cacgaaagat atcgctaaag ctgatatcgc ccgcatcgtg agactctgcg
    41521 cgatgacaat atttatcgct cacccggcaa aacgggttgg taatattgcc cgcattaccg
    41581 gcgtagcccc atgccatccc gagggtgaaa tcaaatggcc ccgcttgctt gctggccacc
    41641 agatactcac cgtcaaacag acccgtacca gcaatatcac gtttaccaaa cgccacttgc
    41701 ggtagccagt aaccttcttc ccacaagcga agtttaaaat cgaaagattt gtctttgtat
    41761 gactgatcgc cgctgaaatc ttcccactgg ctatatttac gtgtgcgcac atccgtataa
    41821 cgaatggtgc cttccagcca tgggaacagc gccacggagg tggagtagaa acggtattga
    41881 tcgttatccc ggtagttgac gctgaattca ccttccggtg cgatgcgcgc atttggcatc
    41941 tgcaacaatc ctgtgccgcc aaagtccgat tgcgacgaac ccagcggatc cgggtaggtt
    42001 aatacttcag catgacaggc actggagacg gcaatggcca ggcagcttaa aagataagaa
    42061 ttcttcttca tcagtcagga acacgttgcg ttaaaacaga cacaatctgg tcatttaaat
    42121 cagcatattt ttcgggcagt acatgcgcag aaaagcctaa ccaaagctgg ctgccaggcg
    42181 gtggttccac atgtctttta ttccacaggg caacaggggc gacgacagtc tcaccctcag
    42241 gagtaatcac gataacgtta ttcttgtccg cgcctgcaag acgggggtga tcttgcagat
    42301 aatcggtaac gctacggcct gcctgccagg gcaattgccc agccccagaa accgcaccca
    42361 gcagagtaat agtcaccgga cgctgtacgg tatagagcgt atagtccccg accagcgggg
    42421 gattgctatt ctcatccact cgtacaaaat cagggtccag tttgaccgct aagcgaccag
    42481 tgatattgag attgagtaac tgctggcgca cggatttaat cgttgccgcc acatcgtcgt
    42541 cagcttctgc ttcccaggaa gcaagctgtg ccatgacatg ttggtagtct ttaagcgctt
    42601 tagctttcgc cgcagagtcg gtcagcaagg caccaggcca ccagaggcga tcgcgcagtt
    42661 gcggttgtgt caccaactgg gcaacatttt ccacggggcc aacggaaagt gtctgttgtt
    42721 cgccaggcag ataaatagtc accgttcctt gcgcaaaggc atggggtgtc attacgtaaa
    42781 gtacgctggc aatgaaatac gactgtaatt tattcatggt gcgggtttaa gaaacgtcat
    42841 ttctacggga ataacccctg cgcctaacat ttgccgactt tgacgaacct ggccagtagc
    42901 cgaatcgacc cagaatgtgt tttgccactg tttttctggc agcgtggaag tcacctcttc
    42961 ctgccagaca ttacacagca catcgcgacc cgcaatatgc agcgtctcta atccagcaaa
    43021 ggaaaaagtg gaactcaggg tcgcagagcg aaaatggttg tcttcactcc agataatatc
    43081 gcgcgtccag cggctgccgt cttttattgc cagcgcgtta cgcagagggt cctgcccgga
    43141 atttgtcact tccagcagat tattattaag tttgacggtc tttaaaagac gtccattgtg
    43201 ggtaaccagc atggcgttat cctgggataa ccatttgctt tgttcttgtt ctatatatcc
    43261 cagtaccaca aagattcgtt gcccttcatt aaggcgtaaa tacatcgtgg aataaggcaa
    43321 cgcctggatc tgctgatccg ctacggtgat atcctgatta tcgaaaaggc tggcgcgaaa
    43381 tgtatcgacc atactttgct ggctgtgcgt acatcccgca agaaatagtg cgaaaatcga
    43441 taaaataaga gggcgcacct ttaatccttg aaaggttaat taaaaaaatg caaatctcta
    43501 aagcccggta cgaatatcgc aaccgggtat tatttaactt taatttcgac gcgtttaagc
    43561 agaattagcg cgtagtggac gtggtggttg aggtgttatg agaaccgcca tcgccactgt
    43621 catttgctgc aactactgcc aacgtcgcca caccaacagc cgttgccaca ccgacaactg
    43681 tcgccgtgcc gacagcgcca gcagccacgc caccagtggt agcagccgtc gtggttgcac
    43741 cggtagtggt cgttgtggtg ccagtagtac ccgtcgttgc ctcggtcccg tttgctgctt
    43801 caggggcagc aaacgcagct ggcgtcgtca acatgaaggc cataaggatt gcagaaagct
    43861 tgtgtttcat aacttttcct ttattcatcg catggacaat acgtacgctt gagatcagga
    43921 ttgtctactt tgagtataac atccttgtga aatctccctt tcctcatacc acttgcacta
    43981 tatacagcgg gtgagaaagg cgcgcagagg gaaatataag attgttaact attcccctct
    44041 ggaaatacga cttgtgagca taagataaat cttagcaaaa tacaaattaa agataagaaa
    44101 attgttaaga aagctattaa ctattgttgg caatatagtt tcaagtggta ctataactcg
    44161 ctgttaagtg gcatttataa ctattagttg tagagatatt cggttaaatg gattagtttt
    44221 ccggtgaaaa agtaacttgt gttataatac atggtgttga ctatttaact atgccaataa
    44281 aaccagtcag ctttaaacaa gcagcgtcat attaagagag ataaacattt gccgctgttg
    44341 gtcctcgcag gccatttacg cggcaaaatt ccacgcgtaa tcctggtata agcacttctg
    44401 cgtcgcgggg agtgaatgcg gaaatatgga cctgaacttc tttacgaccg tcggagggga
    44461 taatgaatcc tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg
    44521 acaaacaaat tccttactga aaatactgcg ctgcactata cggggttaat aaaataaagc
    44581 cagcgatatt taagaccgcc ggacggctaa aataaaattt gcttaatctc aattatcatg
    44641 cgttaatagc tgcgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt
    44701 tctcattatc aggcattatc cccttctttt gagtctctct cctgaacact aagtagtttc
    44761 tgtattaaag ccctgtttgc cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa
    44821 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
    44881 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
    44941 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
    45001 agcgaacgta gttacgctct aaggttgccg ttattactca acatctccat tttcgctgtc
    45061 catgttgtca tggttcacag taccgcacat cggcattcga tgtgacggag cgaaaccctt
    45121 tgggcgctaa gtgtattttt tgtaaatcga cgatgatcac ctttgataac gtcgcgctac
    45181 aaatacgcac tgaccatgca ccgctggatt tcacaaaata atatcaggct ccctcgtgga
    45241 gcctttttta tatctgtctt attttttttc aacgctgtat gtatagtaag cgataacctg
    45301 ttgattattg aatctttcgg ggagatggct tataacattt cttacctgac cagggtaccg
    45361 ggaaccaaca ccttactggc gtgttgctgt cttttaagac cagaagaggt taacagtgaa
    45421 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
    45481 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca ccgcgcgaga
    45541 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
    45601 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
    45661 gcggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
    45721 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
    45781 acaacgcaaa ggctttttta tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg
    45841 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
    45901 ttaatttatc gaccgggatc cgtgatgggc aattttttgc gcatttgcca cagtcgatac
    45961 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
    46021 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
    46081 aaacggtcac gctgattaat gtcgcggtcc ccatataacg aaagaaatcg agcattttaa
    46141 cgtccatcac cacgctgtag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
    46201 ataacgcgat atagagaaaa aaactcaaca gcaaatactt tacaccgcgc agaggaatat
    46261 ccagccagcg gggaaggaca aattgccgac caaaaagttt gttaccgaga gcgccgatta
    46321 attcagaaag cgtaccaacc gggcataacc atgaacaaaa ggcctttttg agtaatagac
    46381 tgatgacgat aaaagcgacc aataacagca ttgcggcggc gtggacggac ggtaactgac
    46441 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
    46501 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
    46561 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
    46621 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
    46681 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
    46741 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
    46801 tttcttattc aaccatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
    46861 gggggcagat aatggctgct gctccagttg tgcgcatagc tggctggcgc tgcgcattcc
    46921 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
    46981 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
    47041 cagccattcg tggatcttct ccgtccccat taattgagca tcttcattga gttgcgatac
    47101 atcaagtggt atatcgttat tgacttgcag ttggagatag tgctccagta actgaccgag
    47161 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
    47221 cagcgtttcg tcaatgacat gagcgctaaa gccaatcaaa accagcgacg gatattgctg
    47281 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
    47341 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
    47401 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
    47461 atcttcaatt aacagcaaac gtaaaccgtc cagacgcacc gcctgattgg ctgtttttgg
    47521 cgcgggtgct gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccag
    47581 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
    47641 ccccagcccg gtgccgccgc gtttgccact tacctgaata aatggctgga agatttctgc
    47701 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcacc
    47761 atcagtacgg ctacgtaaaa cgatctgccc ttcgtcagta aaacgcaggg cgttgctcaa
    47821 caggttggta atgacctgac gaatacgccg cggatcgccc attaacgcag tcggtacatc
    47881 gtcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccac tcattaattg
    47941 cagggtactt tccagcagcg gtcgcggttc aaagggttca tcgctgaccg aaacattctt
    48001 gccgcctgct tcgatagccg aataatcgag aatatcgttc aggatggtca gcaacgattc
    48061 gccagagtcg gtaatcgccc gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
    48121 cagtagctgt gcggtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
    48181 cgccagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
    48241 ttctatcacc agttcctgca attcagctgt acgtgctttg acctgcgccg ccagctgttc
    48301 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
    48361 atccagctcc cgtacgccag cggtttccgg aaaaggggag tcaatatcac cgtccagcag
    48421 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg actgagcgat aaaccacgcg
    48481 ccagaggatc agaatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
    48541 cccgcgtgca ctggcttttt ccagatgcgc cagtccatgc tgattgcgca gctcaatggt
    48601 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
    48661 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca gcaaatcgct
    48721 atattggcta acggtagtta acgttgttgc gacctgagca cgaactcccg gatcttcaat
    48781 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
    48841 cgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
    48901 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
    48961 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
    49021 ggtcgctccg gcggaggttg ccgcattatt cgcctgacct tgcgccaggc gtgcgatctc
    49081 atcggcggca gcgactatct gctgactgag tcgctgttgt tgctggcgca gttgcaaacg
    49141 ctgccccacc agttcccctt gctgacgtaa cgaacgggag atctcctgct cctgttgttc
    49201 aatagcggtg gtatcaaaac cttgttcccg taacgcttgc agcaacgcat taatcttcag
    49261 gctttgtgcg gtgagcattc gcccctgcgc ctgccacatc ttttcgttat cggcactggt
    49321 taggttctgc gcggcgaaaa gttcccaggc gctggcttcg ctcaactggc gcgccatatt
    49381 catggtagga atcaatgcct gagtattgtc tttttccacc tggctgataa agcgcaggtt
    49441 gtaccatccc accagggtac tggtcagggt taacagcgcc atcagggcaa agcccatcca
    49501 gagtcttcgg gtcagggtta aattcacggt cggtgcactt aaggtgaaaa atgttgagtc
    49561 gcaaagagga atgcatctag cataaagcct tattattgat gaggctatca tgcgcgtact
    49621 gctattttta cttctttccc ttttcatgtt gccggcattt tcggctgata acctgttgcg
    49681 ctggcatgat gcgcagcatt tcacggtgca atcctctacg ccgcttaaag ccaaacgcgc
    49741 ctggaaactg tgcgcgcttt atcccagcct gaaagattca tactggttat cgttgaacta
    49801 tggtatgcag gaggctgctc gccgctacgg tgtggattta aaagtgctgg aggcaggcgg
    49861 ctacagccag ttggctaccc agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga
    49921 ggccattttg ctcggtagta gcacgacgtc atttcccgat ctgcaaaagc aggtagcaag
    49981 tctgccggtg atcgaactgg taaatgctat tgatgctccc caggtgaaaa gccgcgttgg
    50041 tgtaccctgg tttcagatgg gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg
    50101 taaaccactg aacgtgctgt tgatgcccgg acccgataac gccgggggca gtaaggagat
    50161 ggtcgagggt tttcgcgcag ccattgccgg aagcccggtg cgtattgttg atattgcgct
    50221 tggtgataac gatattgaaa tccagcgtaa cctgttgcag gagatgctgg aacgccatcc
    50281 agaaatcgac gtcgttgccg gaacggccat tgcggcagag gcggcaatgg gggaagggcg
    50341 taacctgaaa acgccgctta ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg
    50401 gctgaagcgg ggaagagtga ttatggctgc cagcgatcaa atggtctggc agggggaact
    50461 ggcagttgag caggccatca ggcaattaca ggggcaatcg gtgtctgata atgtcagccc
    50521 accgatttta gttctgacgc cgaaaaatgc cgaccgcgaa catatccgcc gctcgctgtc
    50581 gccaggggga tttcgtccgg tctattttta tcagcacaca tcagcggcta agaaataacc
    50641 ttcgccatgt tgcgtcacca gtaaatccgc gctgagttta tggcgtaaac gacgaattaa
    50701 cacatcgacg gtgcgcaggt cagggttttc cacccgacgc gcagagagca tacgaagcag
    50761 acgttcacgg ctgagaattt cgcccggatt ggtcacaaat gccaccaaca tttcatactc
    50821 tgcgcgggtc agtttaatcg gctcgccatc ccgctccagc gtatggcgcg acacattcag
    50881 gcaataaccg gcaaagcgat agcagttgtc ctgagtgagc ggttgaactt gtcgcgcgag
    50941 gtcgattcgc cagagcagat tttttacccg tactaccagt tcgcgcagtt ccagcggttt
    51001 ggtgacgtaa tcgtctgcgc ccatttccag cccaacaata cggtcaatcc gatcgctgcg
    51061 tccggtaacc agaataatcc ccaccgttga gcgttctcgc agggcgcggg ttaacatcag
    51121 gccattttca tcgggcaagt tgatatccag cagaattaaa tccaccggct gattctgcat
    51181 aatttcccgt agcccggcac cgctcgccgt aacggaaacg gtatacccct cctgagtgaa
    51241 gtaggattgt aatcgcgcct gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg
    51301 catcagaggg ttttactcat tctgttcata tctgttcata ttctgccgta agccattcat
    51361 cctgaccagt gccgctgttc atatttgctc attaagatcg cttcactaaa ccataatttt
    51421 acaggggcta ttatgcggaa actctggaac gcgctacgcc gacccagtgc tcgttggtcg
    51481 gtactggcgc tggtcgctat tgggattgtg attggcattt cgctgattgt attgccacac
    51541 gttgggatca aagtcaccag cacaaccgaa ttttgtgtca gttgccacag tatgcaaccg
    51601 gtgtatgaag aatataaaca gtcggtgcat ttccagaacg cctccggcgt gcgagctgaa
    51661 tgccatgact gtcatatccc gccggatatt ccaggcatgg tgaagcgcaa actggaagcg
    51721 agcaacgata tctaccagac ctttattgcc cactccattg atacacctga aaaatttgaa
    51781 gccaaacgcg cggaacttgc cgagcgtgaa tgggcgcgaa tgaaagaaaa caactcggca
    51841 acctgtcgct cctgccataa ctacgatgcg atggatcatg cgaagcagca tcctgaagcg
    51901 gcacgtcaga tgaaggtggc agcgaaagat aatcaatcct gcatcgactg tcataaaggt
    51961 attgcccacc agttaccgga tatgagtagc ggcttccgta agcagttcga tgagctgcgc
    52021 gccggtgcta atgacagtgg cgacacgctg tactctattg atattaagcc gatttatgcg
    52081 gcgaaaggcg ataaagaagc ctcaggttct ctgctgcctg cctcggaagt gaaagtcctt
    52141 aaacgtgacg gcgactggct gcaaattgaa attaccggct ggacagaaag cgccggacgt
    52201 cagcgtgtac tcacccaatt cccaggtaaa cgcatctttg ttgcctcgat tcgtggtgat
    52261 gtgcagcagc aggtgaaaac gctggagaaa accaccgttg ccgacaccaa taccgagtgg
    52321 agcaagttgc aggccaccgc gtggatgaag aaaggcgaca tggtgaacga tatcaaaccg
    52381 atctgggcat atgcggattc gttgtacaac ggcacctgta accagtgcca cggcgcaccg
    52441 gaaatctctc actttgacgc taacggctgg attggcacgc tcaacggcat gattggcttt
    52501 accagcctcg ataaacgtga agaacgcacc ttgttgaaat atctgcaaat gaatgcgtct
    52561 gacaccgcag gtaaggctca cggcgataag aaggaagaaa aataatgaac aataacgatc
    52621 tctttcaggc atcacgtcgg cgttttctgg cacaactcgg cggcttaacc gtcgccggga
    52681 tgctggggcc gtcattgtta acgccgcgcc gtgcgactgc ggcgcaagcg gcgactgagg
    52741 ctgtcatctc gaaagagggt attcttaccg ggtcgcactg gggggctatc cgcgcgacgg
    52801 tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat ccgtcgaaaa
    52861 tgatcgccgg attgccggac cacgtacaca acgcggcgcg tattcgttat ccgatggtac
    52921 gcgtggactg gctgcgtaag cgccatctga gcgacacctc ccagcgcggt gataaccgtt
    52981 ttgtgcgtgt gagctgggat gaagccctcg acatgttcta tgaagaactg gaacgcgtac
    53041 agaaaactca cgggccgagt gccttgctga ccgccagtgg ttggcaatcg acggggatgt
    53101 tccataacgc ttcggggatg ctggcgaaag ctattgcctt gcatggtaat agcgttggta
    53161 cgggcggaga ttactctact ggtgctgcgc aggtgatcct gccgcgcgta gtcggttcga
    53221 tggaagtgta tgaacagcaa acctcctggc cgctggtatt gaagaatagc aaaaccattg
    53281 tgctgtgggg ctccgatttg ctgaaaaacc agcaagcgaa ctggtggtgc ccggatcacg
    53341 atgtttatga atattacgcg cagttgaaag cgaaagtcgc cgccggtgaa attgaggtca
    53401 tcagcatcga tccggttgtt acatccaccc atgagtatct ggggcgcgag catgtgaagc
    53461 acattgcggt taacccgcaa actgacgtgc cgctgcaact ggcgctggcg catacgctgt
    53521 acagtgaaaa cctgtacgac aaaaacttcc tcgctaacta ctgtgtgggt tttgagcagt
    53581 tcctgccgta tctgctgggt gagaaagacg gtcagccgaa agatgccgca tgggctgaaa
    53641 aactgaccgg cattgatgcc gaaaccattc gtggactggc gcggcagatg gcggcgaaca
    53701 gaacgcagat tattgctggc tggtgcgtac agcgtatgca gcacggtgaa cagtgggcgt
    53761 ggatgattgt ggttctggcg gcgatgctgg ggcaaattgg cctgccaggt ggtggctttg
    53821 gttttggctg gcactataac ggcgcaggca cgccggggcg taaaggcgtt attctgagtg
    53881 gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgattac aaaggctaca
    53941 gcagcaccat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg aaagtgatca
    54001 actggaacgg taaatcggta aaactgccgc cgctgaaaat gtgtattttt gccggaacta
    54061 acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc aagctggaaa
    54121 cggttatcgc catagataac cagtggacct caacctgccg ctttgccgat atcgtgctgc
    54181 ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac tccaaccgtg
    54241 gcattatcgc catgaaacag gtcgtgccgc cgcagttcga ggcgcgcaac gactttgata
    54301 ttttccgtga gctgtgccgc cgctttaatc gcgaagaagc ctttaccgaa gggctggacg
    54361 aaatgggctg gctgaaacgc atctggcagg aaggtgttca gcaaggcaaa ggacgcggcg
    54421 ttcatttgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag tttgaccatc
    54481 cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa ccgctgggca
    54541 cgccgagtgg cctgattgag atctattcga aaaccatcgc cgatatgaac tacgacgatt
    54601 gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg cctggctcgc
    54661 agacgtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac tcgcagttat
    54721 gtgagtcgga aactctgcgt cagcaatata cggtagcggg taaagagcca gtgttcatta
    54781 acccgcagga tgccagcgcg cgcggtattc gtcacggtga tgtggtacgt gtctttaacg
    54841 cccgtggtca ggtgttggca ggggctgtgg tttctgaccg ctatgcaccc ggcgtggcgc
    54901 gaattcatga aggggcatgg tacgatcctg ataaaggcgg tgagcctggt gcgctgtgca
    54961 aatacggtaa ccccaacgtg ttgaccatcg acatcggtac ttcacaactg gcgcaggcga
    55021 ccagtgcgca cactacgctg gtggaaattg agaagtacaa cggaacagtg gatcaggtga
    55081 cggcgtttaa cggccccgtg gagatggtgg cgcagtgcga atatgttccc gcgtcgcagg
    55141 tgaaatcatg accacgctga cagcacaaca gatagcctgt gtttacgcct ggctggcgca
    55201 gttgttctcc cgtgaactgg acgatgaaca actgacgcag atcgccagtg cgcagttggc
    55261 tgaatggttt tcgttgctga aaagcgaacc gccgctcact gcggcggtgg atgagctgga
    55321 aaaccgtgtt gccacgctga cagtacgtga cgatgcccgt ctggaactgg ccgctgactt
    55381 ttgcggcctg tttctgatga ctgacaaaca agcggcgctg ccgtatgcat cggcctacaa
    55441 acaggacgag caagagatta aacgcttgtt agttgaggca gggatggaaa ccagcggcaa
    55501 tttcaacgaa ccggcagatc atctggcgat ctatctcgag ttgttgagtc atctgcattt
    55561 ttcgctggga gaggggacgg ttcctgcgcg aagaatcgac agtttacggc aaaaaacact
    55621 cacggcgctg cggcaatggt taccagagtt tgttgcgcgt tgccatcagt atgacagctt
    55681 tggtttttac gcggcactaa gccagttatt gctggtgtta gttgagggcg accaccaaaa
    55741 cagataacgt cgtttgttgg cctgataaga cgcgtttagc gtcgcatcag gcattagggc
    55801 accgccgccg gatgcggcgt gaacgcctta tccggcccac aggaaaagta ctctttgcag
    55861 gcaggctaag atgcgtcatc acatccggca aacacacatc acggatgagg tacaaaccgg
    55921 gaaagccgct ggcgcagcag gcggttttcc tgcttcaagt gcgcaatatc atccattaac
    55981 gtcagcgcca ccgcgatccc cggccagtcc agagccagtt catgacgtag acgtaccgcg
    56041 cgttgcacca caatggcggc atggtcgtca aatacccagg ttgtttcctg gatctcacgc
    56101 ggttcaacca cccccaaacc gacaatttca ttcaattcct cttcagagat gccggtatgc
    56161 aggcaaaatt cggtaatagt aaaagtcacc gtaacattag ccattatgct ttcccccaat
    56221 ctttacgtgg atcaaaagac gactgggcgt ctgccagttg ctgccacagc gcggcagtgt
    56281 tttcatccgg tttcggcggc atcacgattt tcagtaccgc atacagatcg ccggtctgtt
    56341 ttttgctcac cagacctttg cctttaacgc gcaatcgttg cccggcctgg ctgccaggcg
    56401 ggatagtcag caaaatgctt tctttcagtg ttggaacggt gactttagca cccagcgccg
    56461 cttcccacgg gctaaccggc accacaattt ccagatcctg gccgacaata tcaaacagcg
    56521 gatgtggcgc aatatgaatc accagccaca ggtcgccatt tggaccgccg ttttcgcccg
    56581 gcgtcccctg gcctttcagg cggatacgtt gaccattgcc gacgcctgcc gggatcttca
    56641 cattcagcgt tttcgggatt tcctgttcga tcatgccaaa ggcgttataa accggcaggt
    56701 tatagctgat ggtacgctta tgctcagtaa gcgtttcttc gaggaatacc gccacttcga
    56761 tttcaatatc gtggccgcgt gtggcggggc gttgacggct ctggcgggca tgctgaccga
    56821 aaattgacga gaagatatca tcaaaatctt cggcgttaaa actctgaccg tcgccatggt
    56881 ggaactgacg gttaaattgc ggatcgttgc gatgttgcca catctgatca tactcagcgc
    56941 gacgttgttc atcacttaac acttcccagg cttcagcgac ctctttgaag cgggcttcgg
    57001 catccggttc tttgctgaca tcaggatggt atttacgggc cagtcgacga taggcggtct
    57061 tgattgtctt gagatcgtcc gtcggtttca cgcccatgat ggcgtaataa tcctttaatt
    57121 ccatagcgtt atctcgcgta aatcaacaca aattgaagga acccctgtaa ggtaactcct
    57181 ataagtgtag ggtaatcctc aaaatttcat atgccaacac agaatatgtt attgaaatca
    57241 tcgcggagag gatgtcgcca tcaagatggg ttgctgaaca tattttaaac aggtgaaaaa
    57301 gggtgagcga tttttgatag ttgaaccagg cactttaagt ttaactaggg cgtcattatt
    57361 tattaaattt tataggcgct atatatgggt agtaatttac atggaattag ttgcactgca
    57421 aataattatt tgaaacaggc ctggaacgat ataaaaaatg agtacgaaaa aaatcaaaca
    57481 tattcaatca cgctttttga aaacacactg gtgtgtttta tgcggttgta caatgaactc
    57541 agacgtaaag taaatgaaga ggatactcca tgtctggaat gtgaatcgct agaaaaacaa
    57601 ttatatgaaa tgcaaaat
//
LOCUS       DAZFFG010000017        56983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFFG010000017 DAZFFG010000000
VERSION     DAZFFG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.017"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            261..383
                     /locus_tag="ACHJ7J_001344"
                     /pseudo
     CDS             261..383
                     /locus_tag="ACHJ7J_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            504..1244
                     /locus_tag="ACHJ7J_001345"
     CDS             504..1244
                     /locus_tag="ACHJ7J_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4999603.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(1523..2500)
                     /locus_tag="ACHJ7J_001346"
     CDS             complement(1523..2500)
                     /locus_tag="ACHJ7J_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO4999604.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3278..3874)
                     /locus_tag="ACHJ7J_001347"
     CDS             complement(3278..3874)
                     /locus_tag="ACHJ7J_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999605.1"
                     /translation="MSAVTSRGPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSG
                     EAPVYGPNLRASCRRMEASGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAE
                     QRAAEVVVLPLVPASGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNR
                     EGRVVRREGDPLEVAQWLQACHDAGIKVRVQVNESVTP"
     gene            complement(3862..4131)
                     /locus_tag="ACHJ7J_001348"
     CDS             complement(3862..4131)
                     /locus_tag="ACHJ7J_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999606.1"
                     /translation="MDEKVYFRLSYETMTGDTEDFINACLERANRADCNDTDAEIARA
                     RSAIELWYCLALAGRAPDDVADRDHLRFTEMLLRAPTAEQRACQL"
     gene            complement(4451..5383)
                     /locus_tag="ACHJ7J_001349"
     CDS             complement(4451..5383)
                     /locus_tag="ACHJ7J_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-4TM family putative pore-forming effector"
                     /protein_id="HFO4999607.1"
                     /translation="MNAIYTNQSTRENLDLLYAQARVYDRVKNWNRLNFLFSIIVPLL
                     LSFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCR
                     VLGIRRNELKVEEIPRDDIIREAAYFRDSPEKARKQFGEEGWYVSKVYDAPQAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYVVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLGYTLRNIQDEMFAYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQK"
     gene            complement(5387..6382)
                     /locus_tag="ACHJ7J_001350"
     CDS             complement(5387..6382)
                     /locus_tag="ACHJ7J_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFO4999608.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGS
                     RLHSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
     gene            7088..7306
                     /gene="ccdA"
                     /locus_tag="ACHJ7J_001351"
     CDS             7088..7306
                     /gene="ccdA"
                     /locus_tag="ACHJ7J_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO4999609.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            7308..7613
                     /gene="ccdB"
                     /locus_tag="ACHJ7J_001352"
     CDS             7308..7613
                     /gene="ccdB"
                     /locus_tag="ACHJ7J_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO4999610.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            7614..8422
                     /locus_tag="ACHJ7J_001353"
                     /pseudo
     CDS             7614..8422
                     /locus_tag="ACHJ7J_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8602..9246
                     /locus_tag="ACHJ7J_001354"
     CDS             8602..9246
                     /locus_tag="ACHJ7J_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO4999611.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            9333..9641
                     /locus_tag="ACHJ7J_001355"
     CDS             9333..9641
                     /locus_tag="ACHJ7J_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO4999612.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATVPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            10053..11033
                     /locus_tag="ACHJ7J_001356"
     CDS             10053..11033
                     /locus_tag="ACHJ7J_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO4999613.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGGDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            11026..11442
                     /locus_tag="ACHJ7J_001357"
     CDS             11026..11442
                     /locus_tag="ACHJ7J_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO4999614.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(11444..12718)
                     /gene="umuC"
                     /locus_tag="ACHJ7J_001358"
     CDS             complement(11444..12718)
                     /gene="umuC"
                     /locus_tag="ACHJ7J_001358"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HFO4999615.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPET
                     KALGIRMGQPWFQVRQMHLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLREVNRCISPEVFGHQLREQVKSWAGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GMVALTAENRNRIFKLLGLQPVGKVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAIHQAVVQYAERAA
                     EKLRGERQYCRQVATFIRTSLFAVKAPCYSNSAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPHKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(12718..13155)
                     /gene="umuD"
                     /locus_tag="ACHJ7J_001359"
     CDS             complement(12718..13155)
                     /gene="umuD"
                     /locus_tag="ACHJ7J_001359"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO4999616.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLHPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(13152..13400)
                     /locus_tag="ACHJ7J_001360"
     CDS             complement(13152..13400)
                     /locus_tag="ACHJ7J_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4999617.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            13818..14720
                     /locus_tag="ACHJ7J_001361"
     CDS             13818..14720
                     /locus_tag="ACHJ7J_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO4999618.1"
                     /translation="MYFSAEPAQITEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTVAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWQQSG
                     TGQRRWEGLPDEVRENITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAALSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(14671..14901)
                     /locus_tag="ACHJ7J_001362"
     CDS             complement(14671..14901)
                     /locus_tag="ACHJ7J_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFO4999619.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRSCRAASAGAVKRGARRH
                     PLPEKKRAVARPSPSYFIHRNAPPCRLSTPAR"
     gene            14802..15029
                     /locus_tag="ACHJ7J_001363"
     CDS             14802..15029
                     /locus_tag="ACHJ7J_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999620.1"
                     /translation="MTRRGRNAACGQCPASGRARDGRFYRSRLASVTVYASPSPFSDE
                     RPSSRFRGIFSPSNRRRLRYSTVGLTRYRTW"
     gene            15106..15789
                     /locus_tag="ACHJ7J_001364"
     CDS             15106..15789
                     /locus_tag="ACHJ7J_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO4999621.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALLVSFYGWNRIDRFMAAWKRAGFSVVGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            15790..16011
                     /locus_tag="ACHJ7J_001365"
     CDS             15790..16011
                     /locus_tag="ACHJ7J_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999622.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANHLLMEAYTEILELDACFKD"
     gene            15992..16459
                     /locus_tag="ACHJ7J_001366"
     CDS             15992..16459
                     /locus_tag="ACHJ7J_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4999623.1"
                     /translation="MRALKIEKETRMYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQ
                     ALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTFPAE
                     LLASLIQTAEQALWKREWAARDYGLAVPECVIRRQAVVNQARALLKNNTHEND"
     gene            16504..17274
                     /locus_tag="ACHJ7J_001367"
     CDS             16504..17274
                     /locus_tag="ACHJ7J_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999624.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPDWISIYVR
                     LDAPRLATLLINRHDGVLLPILASAVQKMTGTGAEVVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLAAVRDAIRSICYQVAEDARGIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPEVLNAILNRGARFSSVEMYLVSECVEHILASGLVCDVLR
                     IPDEPPRRWFDRDILREVVLEARDEIRSMADALAKIRG"
     gene            17689..18114
                     /locus_tag="ACHJ7J_001368"
     CDS             17689..18114
                     /locus_tag="ACHJ7J_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4999625.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAKKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            18161..18583
                     /locus_tag="ACHJ7J_001369"
     CDS             18161..18583
                     /locus_tag="ACHJ7J_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4999626.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDTFVREEREAMDVVLQALDGEHMS"
     gene            18580..18771
                     /locus_tag="ACHJ7J_001370"
     CDS             18580..18771
                     /locus_tag="ACHJ7J_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999627.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGSQYILQGGRNR"
     gene            complement(18947..19231)
                     /locus_tag="ACHJ7J_001371"
                     /pseudo
     CDS             complement(18947..19231)
                     /locus_tag="ACHJ7J_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19744..19974
                     /locus_tag="ACHJ7J_001372"
     CDS             19744..19974
                     /locus_tag="ACHJ7J_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999628.1"
                     /translation="MTISIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFYRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            20026..21387
                     /locus_tag="ACHJ7J_001373"
     CDS             20026..21387
                     /locus_tag="ACHJ7J_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO4999629.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKFKERPDVRWRRIKKIEADLRKSEKNIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYECAMLDESGSVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            21434..21997
                     /locus_tag="ACHJ7J_001374"
     CDS             21434..21997
                     /locus_tag="ACHJ7J_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4999630.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTSVPTMIIRLRA"
     gene            complement(22083..22568)
                     /locus_tag="ACHJ7J_001375"
     CDS             complement(22083..22568)
                     /locus_tag="ACHJ7J_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999631.1"
                     /translation="MAVSAPCDRGLNRCRAPDTTTGRHPPQRREARFAKRRCALHPGG
                     LLPGCQDRRPRRDRGGATGTVAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKRH
                     KQENNKTDSNQSSRKKKNSSSSSSSSSSRNNNIISNRQMTGLKRQKPTAERPRNERGG
                     A"
     gene            22828..23367
                     /locus_tag="ACHJ7J_001376"
     CDS             22828..23367
                     /locus_tag="ACHJ7J_001376"
                     /inference="COORDINATES: protein motif:HMM:NF010292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4999632.1"
                     /translation="MAVRGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEAQQFSGQPQPEPLAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGDDYGFSDDIPF"
     gene            23424..23657
                     /locus_tag="ACHJ7J_001377"
     CDS             23424..23657
                     /locus_tag="ACHJ7J_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO4999633.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYWNVFIEDDHGEHFRLV
                     VRNNGALVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            23721..25679
                     /locus_tag="ACHJ7J_001378"
     CDS             23721..25679
                     /locus_tag="ACHJ7J_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO4999634.1"
                     /translation="MSVTESKTKTERKSSRKPAKKQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHVLSGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTSAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRINTEHCQVLALENDTARQVLVFEAACQ
                     SGWGGKPEVQTIRRMVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHIREAEGWEWCAGRMEPVGFCREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMAKVMAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMGSPRPQTDATERADNLADAA"
     gene            25734..26168
                     /gene="psiB"
                     /locus_tag="ACHJ7J_001379"
     CDS             25734..26168
                     /gene="psiB"
                     /locus_tag="ACHJ7J_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO4999635.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSNERRELTHAVMRELDASD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQV"
     gene            26165..26927
                     /locus_tag="ACHJ7J_001380"
                     /pseudo
     CDS             26165..26927
                     /locus_tag="ACHJ7J_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            27100..27249
                     /gene="mok"
                     /locus_tag="ACHJ7J_001381"
                     /pseudo
     CDS             27100..27249
                     /gene="mok"
                     /locus_tag="ACHJ7J_001381"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            27191..27316
                     /locus_tag="ACHJ7J_001382"
     CDS             27191..27316
                     /locus_tag="ACHJ7J_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO4999636.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(27617..27937)
                     /locus_tag="ACHJ7J_001383"
                     /pseudo
     CDS             complement(27617..27937)
                     /locus_tag="ACHJ7J_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27939..28226
                     /locus_tag="ACHJ7J_001384"
     CDS             27939..28226
                     /locus_tag="ACHJ7J_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999637.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYNYALSQGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRALC"
     gene            28344..29165
                     /locus_tag="ACHJ7J_001385"
     CDS             28344..29165
                     /locus_tag="ACHJ7J_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4999638.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMISGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALLVIAEQFRKWKS"
     gene            complement(29461..30063)
                     /locus_tag="ACHJ7J_001386"
     CDS             complement(29461..30063)
                     /locus_tag="ACHJ7J_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO4999639.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGINPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRIPATKKSLTEINSVQSN"
     gene            30374..30757
                     /gene="traM"
                     /locus_tag="ACHJ7J_001387"
     CDS             30374..30757
                     /gene="traM"
                     /locus_tag="ACHJ7J_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO4999640.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQAEFNKVLLECAVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPENDED"
     gene            30951..31643
                     /locus_tag="ACHJ7J_001388"
     CDS             30951..31643
                     /locus_tag="ACHJ7J_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFO4999641.1"
                     /translation="MCALDPRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSKEDKPFSGESYVRLQVEIFLSSLELECQSLGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNEESVVLWQINIFPDYPFFRVEKENYYHRDSYVQSVISNMTAKSLVVF
                     CFYALGYKHINIAKELKITEVASKKRIKKINADINKRLGSFDLFRNKCIKTGVMYEIL
                     NVLSEYMGVKKM"
     gene            31731..31958
                     /gene="traY"
                     /locus_tag="ACHJ7J_001389"
     CDS             31731..31958
                     /gene="traY"
                     /locus_tag="ACHJ7J_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO4999642.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVIEENESTFKEL"
     gene            31992..32351
                     /gene="traA"
                     /locus_tag="ACHJ7J_001390"
     CDS             31992..32351
                     /gene="traA"
                     /locus_tag="ACHJ7J_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO4999643.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGL"
     gene            32366..32677
                     /gene="traL"
                     /locus_tag="ACHJ7J_001391"
     CDS             32366..32677
                     /gene="traL"
                     /locus_tag="ACHJ7J_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO4999644.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            32699..33265
                     /gene="traE"
                     /locus_tag="ACHJ7J_001392"
     CDS             32699..33265
                     /gene="traE"
                     /locus_tag="ACHJ7J_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO4999645.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            33252..33980
                     /gene="traK"
                     /locus_tag="ACHJ7J_001393"
     CDS             33252..33980
                     /gene="traK"
                     /locus_tag="ACHJ7J_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO4999646.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAGAGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPAGQLLAGARMDVYVIRDGESN"
     gene            33980..35407
                     /gene="traB"
                     /locus_tag="ACHJ7J_001394"
     CDS             33980..35407
                     /gene="traB"
                     /locus_tag="ACHJ7J_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO4999647.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            35397..35987
                     /gene="traP"
                     /locus_tag="ACHJ7J_001395"
     CDS             35397..35987
                     /gene="traP"
                     /locus_tag="ACHJ7J_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO4999648.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVGTADEHLK
                     EVYTEDTSLSEKIRKCHVYFPDDRANRNNGDKNEHA"
     gene            35974..36294
                     /locus_tag="ACHJ7J_001396"
     CDS             35974..36294
                     /locus_tag="ACHJ7J_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO4999649.1"
                     /translation="MNMRNINIITARSVPDKSVSDDFMNAVLSNCTTKIVLPAPKVFS
                     SESLPHNFNMAAVGVMKKGMSMRLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            36291..36806
                     /gene="traV"
                     /locus_tag="ACHJ7J_001397"
     CDS             36291..36806
                     /gene="traV"
                     /locus_tag="ACHJ7J_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO4999650.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVQVSSVVPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            36941..37162
                     /gene="traR"
                     /locus_tag="ACHJ7J_001398"
     CDS             36941..37162
                     /gene="traR"
                     /locus_tag="ACHJ7J_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO4999651.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            37155..37631
                     /locus_tag="ACHJ7J_001399"
     CDS             37155..37631
                     /locus_tag="ACHJ7J_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999652.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRRVLSRQELLWLMSLTEDCPDM
                     HQELLNCLLSGKKLTGLDKREIRSLWWEKMDAMQESATRQREQDTIRQFSEESKSE"
     gene            37710..37928
                     /locus_tag="ACHJ7J_001400"
     CDS             37710..37928
                     /locus_tag="ACHJ7J_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999653.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            37956..38303
                     /locus_tag="ACHJ7J_001401"
     CDS             37956..38303
                     /locus_tag="ACHJ7J_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999654.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMLICGLFYNDYLEQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEARRVRENELNKIREKQ
                     LQALSDADSAKER"
     gene            38434..41064
                     /gene="traC"
                     /locus_tag="ACHJ7J_001402"
     CDS             38434..41064
                     /gene="traC"
                     /locus_tag="ACHJ7J_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO4999655.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEILGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTLGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKRSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            41061..41447
                     /gene="trbI"
                     /locus_tag="ACHJ7J_001403"
     CDS             41061..41447
                     /gene="trbI"
                     /locus_tag="ACHJ7J_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO4999656.1"
                     /translation="MSSTQKPADVTAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            41444..42076
                     /gene="traW"
                     /locus_tag="ACHJ7J_001404"
     CDS             41444..42076
                     /gene="traW"
                     /locus_tag="ACHJ7J_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO4999657.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMNAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            42073..43065
                     /gene="traU"
                     /locus_tag="ACHJ7J_001405"
     CDS             42073..43065
                     /gene="traU"
                     /locus_tag="ACHJ7J_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO4999658.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            43092..43400
                     /locus_tag="ACHJ7J_001406"
     CDS             43092..43400
                     /locus_tag="ACHJ7J_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999659.1"
                     /translation="MEKKLSSKYIHMYMSISRVFIRLTGNVLKLFPFAMVICPVVWFY
                     LCPEHIISDIVNDWQDADTLEKILLVKLYLKTVLILTVVSVVVLSLSSDKFMSVKRRK
                     "
     gene            43463..43981
                     /locus_tag="ACHJ7J_001407"
     CDS             43463..43981
                     /locus_tag="ACHJ7J_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999660.1"
                     /translation="MSFLSFLRKISNKEKEIFISEERFFSLLERVSAFDVVREWAENG
                     GAYYPLLECRRLTDIEDVRKSEDILNRLEFIDRRFREFISIMSEYNRNDKSKNRWERE
                     LSEYPPEKIYQVRREGNHLSLKNYGDDFTLDRDTAVATVQITVSRKCITQEEMDELYA
                     LSERLKEKCAGN"
     gene            44008..44097
                     /locus_tag="ACHJ7J_001408"
                     /pseudo
     CDS             44008..44097
                     /locus_tag="ACHJ7J_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
     gene            44189..44368
                     /locus_tag="ACHJ7J_001409"
     CDS             44189..44368
                     /locus_tag="ACHJ7J_001409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999661.1"
                     /translation="MMEKEQKQCGLQLPLHQDVSLPVPRDTFSMEKKVITAEQYRDWQ
                     LIAASLRKKINGERS"
     gene            complement(44365..44604)
                     /locus_tag="ACHJ7J_001410"
     CDS             complement(44365..44604)
                     /locus_tag="ACHJ7J_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999662.1"
                     /translation="MNNNTSEDRPDFSFSYMDNQRADEIKALKILLFSIIRNMPHDTA
                     ISIINNIVNTDNNEALNRLCLEITDAMLNTTKIVD"
     gene            44797..45435
                     /gene="trbC"
                     /locus_tag="ACHJ7J_001411"
     CDS             44797..45435
                     /gene="trbC"
                     /locus_tag="ACHJ7J_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO4999663.1"
                     /translation="MNKKIYILFALTTGLFISAHASESVNTPENRQFLKQQETLSQQL
                     RETPDTQLQAWAEQQVRVNPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKATAEAVLALVKDGATDGVQIDPTLFSQYGI
                     RSVPAMVVFCSQGHDIIRGNLRVGQALEKVAATGDCRQVAQGLLDGTGDKSR"
     gene            45432..45803
                     /locus_tag="ACHJ7J_001412"
     CDS             45432..45803
                     /locus_tag="ACHJ7J_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999664.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESMMPRLLWGWSVGASFNSDLQLCFRLQPEGPHHWY
                     HRYCRRSLRADFRTLVRLLNP"
     gene            45829..46251
                     /locus_tag="ACHJ7J_001413"
     CDS             45829..46251
                     /locus_tag="ACHJ7J_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO4999665.1"
                     /translation="MALTKKQREKLRMKFGGRCAYCGCELPEKGWHADHVQAVLRKSE
                     QCMKAAEKRIFRLKSTGDVFRPEADCPENLVPACAPCNLLKTTYSLEMFRMQVSLQVE
                     RGRRSSVNFRTAERFGLIQVVTKPVVFWFEQYQRESEV"
     gene            46248..48098
                     /gene="traN"
                     /locus_tag="ACHJ7J_001414"
     CDS             46248..48098
                     /gene="traN"
                     /locus_tag="ACHJ7J_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO4999666.1"
                     /translation="MKRLLPLLLGLAVSVVQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVESRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEFCTRT
                     ARSTGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNSTFNVGLANGQSGTFPLSGATGLPVTQGQVLTGSGCTANGNCLSGGNGDR
                     QVYEKLASGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSARSPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            48125..48382
                     /locus_tag="ACHJ7J_001415"
     CDS             48125..48382
                     /locus_tag="ACHJ7J_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO4999667.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKADERKNDERNEDE"
     gene            48375..49118
                     /gene="traF"
                     /locus_tag="ACHJ7J_001416"
     CDS             48375..49118
                     /gene="traF"
                     /locus_tag="ACHJ7J_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO4999668.1"
                     /translation="MNKALLPLLLCCFLFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKQALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            49132..49473
                     /gene="trbA"
                     /locus_tag="ACHJ7J_001417"
     CDS             49132..49473
                     /gene="trbA"
                     /locus_tag="ACHJ7J_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO4999669.1"
                     /translation="MHTGDLCFFTGASLFIVGGVGYFFTGPNNKGVYLLSSFVFFVVN
                     IISVCVLPDNFLFLCGAVMNQCAVPVVSMAVSTMPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(49454..49789)
                     /locus_tag="ACHJ7J_001418"
     CDS             complement(49454..49789)
                     /locus_tag="ACHJ7J_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFO4999670.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            49871..50155
                     /gene="traQ"
                     /locus_tag="ACHJ7J_001419"
     CDS             49871..50155
                     /gene="traQ"
                     /locus_tag="ACHJ7J_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO4999671.1"
                     /translation="MISKHRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
     gene            50142..50687
                     /gene="trbB"
                     /locus_tag="ACHJ7J_001420"
     CDS             50142..50687
                     /gene="trbB"
                     /locus_tag="ACHJ7J_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO4999672.1"
                     /translation="MSLTKLLPVILLIMATGVRASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            50617..50961
                     /gene="trbJ"
                     /locus_tag="ACHJ7J_001421"
     CDS             50617..50961
                     /gene="trbJ"
                     /locus_tag="ACHJ7J_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO4999673.1"
                     /translation="MPPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIAMGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFMGAIVGWISVSEIYYRRMTGLNESS
                     EISEGTYDERKK"
     gene            50958..51338
                     /gene="trbF"
                     /locus_tag="ACHJ7J_001422"
     CDS             50958..51338
                     /gene="trbF"
                     /locus_tag="ACHJ7J_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO4999674.1"
                     /translation="MNDTELEIKQIEREIERSCDNAKKYFWLFVVFFAAGLIVRNVMH
                     DFFSAGIDSWKADPELNNFRYMWNILMYVIPIMLYALAAGFLAAASLSPLCEIIFGRG
                     RIFLMKRRMRRENKLREGGNNASH"
     gene            51325..52698
                     /gene="traH"
                     /locus_tag="ACHJ7J_001423"
     CDS             51325..52698
                     /gene="traH"
                     /locus_tag="ACHJ7J_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO4999675.1"
                     /translation="MLRIKPLLVLCAALLTVTPVALADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAVFQSQVQVQQDALLVVERQMSYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            52695..55520
                     /gene="traG"
                     /locus_tag="ACHJ7J_001424"
     CDS             52695..55520
                     /gene="traG"
                     /locus_tag="ACHJ7J_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO4999676.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVVVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASDDPYTIIFSQPSPLRGVYDNNNNFITCKDASVSLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRELLSDSYSYFYGASQSASQIMRKNV
                     TINALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVAIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLTAVFNKLTLSVLKGYVFALMWFQTWPLLYAILNSAMTFYTKQNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINTTRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPKILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKYQVDNMVTEYRGNIGDTQSSIRGEENIVGRQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEETVKKMIDEKKNQLKGSL"
     gene            55517..56026
                     /gene="traS"
                     /locus_tag="ACHJ7J_001425"
     CDS             55517..56026
                     /gene="traS"
                     /locus_tag="ACHJ7J_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFO4999677.1"
                     /translation="MITHYDIKAETQRLKDVLSVEGVNIPPLLQVIKPGGYVFLWILL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFVIFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIFSFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            56040..56771
                     /gene="traT"
                     /locus_tag="ACHJ7J_001426"
     CDS             56040..56771
                     /gene="traT"
                     /locus_tag="ACHJ7J_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO4999678.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKSTVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
BASE COUNT        14019 a        13570 c        15461 g        13933 t
ORIGIN      
        1 ctttctatga attaatgatt acacttacag tacaaatatt atttgtgtgt aaacatattc
       61 attcaataat gacggtaaga aacttctata agtttaccgc ataatctaac cattaacttc
      121 atttattatc atgcgaacaa aatttattat gcatcaggtt gatgatgtga ttaatcagtc
      181 tgaagcttgg ggaatagata aggatatggt gtcagaaacc ttcagttgtc gttgcaggat
      241 ttttctccgt ttctgccttg tcctggcatt accccaattc tcccaaaaac ggcaaaccag
      301 ccatcagccc gcgccacgac tggcttaaaa cgggatccct gctgcaaaac gcgcctttac
      361 gtatttggtt ttacccgcaa taatgcgtga taagcaaaca aaaggatccg cgtgctgcga
      421 tttattgtcg tggaaggatc cgggcgttca ggtcagaata cctgtatcca taaacagtgc
      481 gtgtgctacg tgaaaaataa ctcatgaaca atgtcattcc cctgcagaat tcaccagaac
      541 gcgtctccct gttacccatt gcgccggggg tggattttgc aacagcgctc tccctgagaa
      601 gaatggccac ttccacaggg gccacaccgg cctacctgct ggccccggaa gtgagtgccc
      661 ttcttttcta tatgccggat cagcgtcacc atatgctgtt cgccaccctc tggaataccg
      721 gaatgcgtat tggcgaagcc cggatgctga cgccggaatc atttgacctg gatggagtaa
      781 gaccgtttgt gcggatcctg tccgaaaaag tgcgtgcgcg acgcggacgc ccgccaaaag
      841 atgaggtccg cctggttccg ctgacagata taagctatgt caggcagatg gaaagctgga
      901 tgatcaccac ccggccccgt cgtcgtgaac cattatgggc cgtgaccgac gaaaccatgc
      961 gcaactggct gaagcaggct gtcagacggg ccgaagctga cggggtacac ttttcgattc
     1021 cggtcacacc acataccttc cggcacagct atatcatgca catgctctat caccgccagc
     1081 cccggaaagt catccaggca ctggctggtc acagggatcc acgttcgatg gaggtttata
     1141 cgcgggtgtt tgcactggat atggctgcca cgctggcagt acctttcaca ggtgacggtc
     1201 gggatgctgc ggagatcctg cgtacactgc ctcccctgaa gtaacggcac ttccttatat
     1261 aagcagtggt cagcagaaca gacataacat aagctggagc aggtagataa gctgtagtga
     1321 gtaaaccatg ttttatgaag ggagcaatgc ctcagcatca ggttacgggg tgactcacgt
     1381 aagggacagg cagatggcag ctcagtcaca ggcagcactg caggaaactg aatataaact
     1441 gcagtgagca gaccactcac tgcacctgat aatataagct gtagtcagta aaggagcact
     1501 cttcactgac tacagtttat gttcagcggg atttgaagag tttttccagg tcatccagtg
     1561 tgatgcccag tttttcaagc agggcaagtt tctccgccat ctccggactg actttttccg
     1621 caggtgaagg tggcaacgga ttttccttac tctcatcatt cggtgctttt aaccggggac
     1681 gccggtagtg aatgcagaag agttttgtcc gcccccgctg gatctccgtg taatcaagat
     1741 atccaatctc gcgcagctgc tccattgccc gtctgaccgt ctggttctgg gaaaatacag
     1801 gagacttcag attgaggcgt gcacgcagcc gcgccagcga tatcggtgcc ggatcccggg
     1861 gcaggctctc tataaaggtg tagagtgcct gggcggactc ccgtcgcttc agggcattaa
     1921 tcgccttaag ctggagaagg acttttctgt caaactggta cagttcaaac aggcggggat
     1981 cagcctgtaa ctgaacaata tcccgctcag tatcgtagta ggctgactgt accagatggg
     2041 tgatgtattc ccgggtgtgc ttctcatcgg tacgggaaaa cgagatcacg gtaccggcaa
     2101 tgcgcttcag ggaagggctg atgcgctcac gcagcctgcg ggatgactgg cttgaaggta
     2161 taccacacag ttttgcaaac tcaacaaaag gcagttcaac tttgtcacca atcacgttat
     2221 ggcgggcaaa ggaatgaatg atccccaccc aggtcttgaa atcgttatcc atatccaggc
     2281 gggggccggt gatctcaacc ttatcgaatc cctcagcacg ggccagggaa agacgtgtca
     2341 gctcttccgt ggcatcagta cgtgacagtg tatttttttt actgttcttc agtgatttaa
     2401 gggtcggtac aaaaacgccc agacgcatca gcgccacagg ttgtacggtg ttgttattgt
     2461 ttggtgtcag tgtcaccagc tcaccggacg acttatccac ctcgccgaac agttttttga
     2521 tgtctgaatt ttcgttttcc agaaagcctc ctgcctgtgg acagtaaaag aataaacaga
     2581 aaatcgtgtg tcagaacccc gtcaactgac tgaagaaaaa cctgcagtct gtatttaaca
     2641 actgagtctg gcgtactgac catagtttat gtcattaact tacttcagta tatgcattac
     2701 tgaatacagc ttatcatgaa cctgttacag tttatccata acatgccaca gcttatgtga
     2761 tcgtgctccc ggaccattaa tgacaaggtt tacagcgata tggggatctt taatggatcc
     2821 tttgttgatc attcatggat ctgtttactg gatcgggcct ctggatatgt ggataaatca
     2881 ttccagaaaa acagcataca ctaccgatta tgaaggcttc agggcgtacc tgctcactgt
     2941 aactacagat atattgcgct tcctcgccgg ttcgcgggct tcgcccggtc accggtctgc
     3001 ggcgagcact gttttttctg aagtctgtct tcacaggtaa tttttgacgg aatctgccgt
     3061 acttcctgag cgcacggtca tatccgaagt acacttcata tgtgtgtttc aatgccaggg
     3121 attgtgtata caaactacag gcaaagtctg gttatcaccg gtggtagcct gctccgatgg
     3181 aaacatggtg aactgattga caccatgccg gataactctg tcagcgccag tgatataaga
     3241 cggtcaaaga tcgtggcgtt gacatgctgg catgttttta cggggtgacg ctttcattaa
     3301 cctgtacacg aacttttatt ccggcatcat gacaggcctg cagccactgc gccacttcca
     3361 gcggatcgcc ctcccggcgt accaccctgc cttctctgtt ccataactgc agacaggtac
     3421 tgccgtcgag acgtaccaca aaatccccac ggcaggcctg atacgtcatg ccgttcagct
     3481 ccaccgccag atcagtggca gaagtgccgt ctgcgggcag acctgatgcc gggaccagcg
     3541 gcagtacgac gacttctgcc gcgcgctgtt ctgcccggag gcggtcctgt tctgccagca
     3601 gcagaggctg tgccacggcc cggccggcgt ccgtcagctc gacagccagc tgcagattcg
     3661 gggcgcgcag ggtgcgaagc cagcctgatg cttccatgcg ccggcaggag gcgcgcaggt
     3721 ttggtccgta gaccggggct tccccgcttc gctccagcac ccgttctatg tcccgagtca
     3781 gcacgggtcc aggccgtttt tcatcaaggg cagccagcac aatcagcacc cgtcgttgca
     3841 gcggcgacgg cccacgggag gttacagctg acatgcgcgt tgttctgctg tcggggcgcg
     3901 caggagcatt tcggtgaacc gcagatggtc cctgtcggcg acgtcgtctg gggcacgccc
     3961 cgcgagtgcc agacagtacc agagttcaat ggcgctgcgt gcgcgggcaa tctccgcatc
     4021 ggtatcgttg cagtccgcac ggtttgcccg ttcaagacat gcgttaatga agtcttccgt
     4081 atcgccggtc atcgtttcat agctgaggcg aaagtatact ttttcatcca tcctgcatgc
     4141 tccgtttgat tatgtacatt aatatgtaaa atcatacata gaataattta tctctacttt
     4201 agtgaatatt atatcagata tgaatttatg tattatgaaa aatatcatat ctagtatgat
     4261 tttatgtgtg tttcttaatt atcatattat ttgcgtcttt tctgccgcgc agatccggtt
     4321 tctgtgaaag ctggatgcta accgtctgag gtgaaggtga ctatgtgttt acgctgacac
     4381 tcaggcaacc agaattgttt atcggatgtg ctccggcata cgtaaatcct acaccggagc
     4441 taaggaggga ttacttctga aggtgaagct ccttcagatt tgtttcaaag taatcaacat
     4501 aaattcgttc atttttgggc ttcattatca gctgaatacc gttgggtacg ggcggacatg
     4561 aggcccggta ggcaaacatc tcatcctgga tattccggag cgtataaccc agttgctctt
     4621 cttctgccct tccggatacc cttatgccgg ataattcctt ctcaacgtac tttttcagct
     4681 tctcgcttct tttcatactg ctgcgattat ccagaaactg aagcaagaaa tagcggatga
     4741 ccggcagtgt gaacaccaca tagaagagga tctcattcag cccggatttc atgacgatgc
     4801 cgtaaacaag catcgcgaca ggcgagacaa taaaagcgga aagataaaat acgtgcagaa
     4861 cctccctgag cgatttgtcc cagccgagat tttttccgtg gcaaaggagc gccattacgg
     4921 cctgcggcgc gtcatacacc ttactgacgt accacccctc ttcgccaaat tgctttcgtg
     4981 ctttttccgg gctgtctcta aaatatgcag cttcccggat gatgtcatcc cgtgggatct
     5041 cctccacctt cagttcatta cgtcggatgc caagtacccg gcagtcgtac atttcctgga
     5101 ttgaggctgc cttcctgcgc agagccgaga tgtgtccact gataacaatg ttaaaggtaa
     5161 gcaccaggag tccatataac cccaacaacg aagacaacag ctctgaatcg acaaattccc
     5221 ggctccggtt gtatacggta acgaaagaaa gcaggagcgg tacgatgatg ctgaagagaa
     5281 aattcagtct gttccagttt tttaccctgt cgtagacacg ggcctgcgca tacagtaaat
     5341 caaggttttc tctggttgac tggtttgtgt aaatggcatt catggttcac ccgtaaacag
     5401 gaaagaacgg cccgaacaca tcgcgccatt tattgatgga tttttcatac tctttattat
     5461 tttcaaacca tctcgcctcc gatacttttt gcgcatcgag ataacatctg tccgatatcg
     5521 ccttacgtgc ctcggctgaa agtgagttga tatcaccctg tatgcctttg ggatcgttga
     5581 cagagtaccg cacggattga ccaaggtggc ggaaaagcgc ttccagctcc atgtcaacaa
     5641 acgatgagca ggatgtcctg ccagcgtaat agtccagaat cagcgtttcc agcaggtagg
     5701 aactcatgga tggcatcgtc ggtcgccgct gccagtattt gactgctctg atgacattga
     5761 gcacattgcc gttattctgc acattgatag tagtgactct gtccctgtct ttacgggggt
     5821 cggtaaactt ccagtggccg ttgccatcgg ggatcaggta gtaagtccgt ccaaaggcat
     5881 cttcgctggt aataaagcag gggacgatgt caaaattcca gtccttgctg accagtttca
     5941 gcgtgacagc ttcctggtta cgcctgatat cagcctgggc gtactgggca atgtcctgca
     6001 agcggttttt aaaggcattc agaattctga cggagcagac ggtgtctgct ccggaatggc
     6061 ggtaactgtg cagacgtgac ccctctccgg aggaggttac agttatccgg tcactcagga
     6121 tggtataggt ggcaccttgc ccggttaagc caaacatcag atcaatatca tcaagcggac
     6181 ggattttggt tcttctggca aaggagccaa aggcaatatg gatcgctgga aaactcaccg
     6241 gaaatttatc gtccttctca aaatcattca tcttaccgat aagccagtcg cgacttgcac
     6301 gcgcgtcatc ggtatctgcc tttttgagat tcacggtatc tttcataaat tcgttaaagg
     6361 cagctattac tgtggtcgcc atgttcagtc ctttaattat ttattgcaaa aggcgcaggg
     6421 ctctgtcgta catctgaaca ttccagtgcc caggaagagt cggtacagtt tattattagt
     6481 acagcaagtt cactaccgtc tcgctctcta caagggcata taatttacat gggaaatatg
     6541 agcctcgttc tttcattgat aattatgcaa agtatttcag ttattaatgc caaataagca
     6601 ataatgcaat taagttcaaa atatattatt agtattgcat tatcagcaca aatccttctt
     6661 ctcctctcct gagatccggt ttccggttgt tacggatctt cctctccccc acacaacgca
     6721 cctcctgtaa gtacaacatg tcgtactgca ttcagctgct catgacacta tatgttgtgt
     6781 tatttccctg acctgtgatg cgccgtgcag gggcggaaaa cagcgatatg attatttccc
     6841 cagcgtggta cacttccgga aacccgtcaa taacagaaaa atcggatctg ccggaaatgg
     6901 tccttcgata attacgcttt gtttttataa gtctgatttt aatttttatt ggttaacata
     6961 atgcaaggta aaataaataa ggcttactaa aagccagata acagtatgtg tattaacgcg
     7021 ctgatttttg cggcataaga atatatactg atatgcatac cagaagtatg tcaaaaagag
     7081 gtgtgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agctgctcaa
     7141 ggcgtatgat gtgaatatct ccggtctggt cagtacgacc atgcagaacg aagcccggcg
     7201 actgcgcgct gaacgctggc aggctgaaaa tcaggaaggg atggcagagg tcgccaggtt
     7261 tattgaaatg aacggctctt ttgccgatga gaacagggac tggtgaaatg cagtttaagg
     7321 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtccag agtgacatta
     7381 ttgacacgcc cgggcgacgg atagtgatcc ccctggccag tgcccgcctg ctgtcagata
     7441 aagtctcccg tgaactttac ccggtggtgc atgtcgggga tgaaagctgg cgcatgatga
     7501 ccaccgatat ggccagtgtt ccggtctccg ttatcgggga agaagtggct gatctcagcc
     7561 accgggaaaa tgacatcaaa aatgccatta acctgatgtt ctggggaata taaatgtcag
     7621 gctccgttat acacagccag tctgcagcca tggtaccggc agtgtattct gccggtacag
     7681 tctccacaac ttcctgttgt cattgattat ccggcagctc tggcacttcg ccagatgtcg
     7741 atggttcatg atgaactgcc gaaatatctg ctggctccgg aagtgagtgc cctgctccat
     7801 tacgtcccgg atctgcgccg caagatgctg ttggccacac tgtggaacac cggtgcgcgc
     7861 attaatgaag cactggcgct gacgcggggg gatttttcgc tcgcgcctcc gtatccgttt
     7921 gtgcagcttg cgaccctgaa acaacgggcc gaaaaagccg ccaggacggc aggaagaatg
     7981 ccagccggcc aagcagactc accggctggt tccgctctcc gacacctggt acgtcagcca
     8041 gctgcagacg atggtcgcaa cactgaaaat tcctctggaa cggcgtaata aacgaaccgg
     8101 caggacagag aaagcgcgga tctgggaagt gacggacaga acggtcaaga cctggattgg
     8161 ggaagcggtt gtcgccgctg ccgctgatgg tgtgacgttc tctgtcccgg tcacgccaca
     8221 tacgttccac cattactatg cgatgcacat gctgtatgcc ggtataccgc tgaaggtcct
     8281 gcagagcctg atggggcata agtccatcag ctcaacggag gtctacacga aggtgtttgc
     8341 gctggatgtg gctgcacggc accgggtgca gttttcgatg cctgagtccg atgcggtgtc
     8401 tatgctcaaa agaattcctt aagctctgac ttttttaaat ccccttagga tatctcatgg
     8461 atatattatc ggtatttcat attctgttga catgcaggac cggtatagag taaaaaaacc
     8521 tctattgagt acctcttgga tatccgaaag gtgtcttttg ggttctttat gggtattaca
     8581 tgtggattct ggagatacac tatgcctgtt attgctaata cacatcccaa aggtggtgtg
     8641 ggtaagacaa cttcatccgt aaacattgtt ggtgaaatga agtctgatac agttgatctg
     8701 gatactcata ccgggctttc tatcattctg gggctgagac ctgaaggaaa agaaatttcc
     8761 gtgaaagtac ccaaaacagt ggatgagtta atcgaaatta tgactcccta caagaacagc
     8821 gataagacgt tacttattga ctgtggggga tttgactcag atcttacccg tactgcgatt
     8881 gcctttgcag actgcgttat tgtcccttca aaagactccc tgactgagcg tattggtttg
     8941 atgcattttg atggagtact ggacgaaatc agctctatca tggggaccga tataactgct
     9001 cacctgtatc tctgtaaagt taatcctaac aagaagaaat tccctaagct tgatgcaatc
     9061 ctgccatcgt tcaaacatct gaagctgatg aagagccgca tttctgctcg tgcagaattt
     9121 gaggatgtca ttgagactgg gatgggaatc actgagtctg ttcatgggcg ctattcggca
     9181 ggaggtaagg aagttattgc ccttatcgaa gaaatcaatc atctgattga aaataacaaa
     9241 cagtaaagtg tatctattgg gtatccatat agtatccttt gggcttttaa tggatatctt
     9301 ttgaatatct aacaaacatc ctgggttatc tcatgagcaa agcgaaattt agtgcacagg
     9361 ctgaaaaact gaaaaacttg gtggattctg ccagattaca aacttcttct tctgccgtaa
     9421 cagcaacagt cccgtctcag ccaaatgaag gtgcagagga agtttcaggt cgtcccgtgg
     9481 ccgccaggac gaaggctaag aatcttaagg ctataccgct gtcgtatttt gaagctcatg
     9541 cggaacttaa agcgaccagt aaaacatctc tggattttag ctcatatatt attgaggcta
     9601 taagagagaa gcttgaacgt gatggtgcaa taggtcaata aactatcaga taccttaagg
     9661 gtatcttatg tgtgtctttt aaatgcgtta tagggattcc ttgtgtacct aaagagtccc
     9721 tattagacat ctttctgtat atggtgacat gattcgtcac tgctttcctt tcctctcagt
     9781 ttcaccattt cattcccttc ccttaacctg aaccatgatt tcaaagatgt ttttatatta
     9841 catttatgtg ttgcatataa cattcataat gtttttgtat ctatgatttc tctggtctta
     9901 aggttgcatg tgcaatatac atgcaattta ttgtgttttt atgttgcttt caccctttca
     9961 gaaagaccat ctttccatta agattgaata tacattgtat gtatgaagtg tgtttgtttt
    10021 ttctatttgt ttatgagcaa cggagactgg ttatgaaaat ctttattgat gatggttcta
    10081 ccaacattaa acttgcctgg ttggaggatg gtgacgtgaa aacgttaatc agccctaaca
    10141 gctttaaacc ggaatggtct tttagtttac tggatgatgc tgccccggct aattacgaga
    10201 tcgacggcga gaagttttca tttgatccat taagtgctga tgctgtcgta acgactgaaa
    10261 cacgctatca gtacagtgat gtgaatgttg tggccattca gcatgcgcta cagcagaccg
    10321 gcctgaaagc gcagcctgtt gatgtcatcg tcacattgcc gatttctgaa taccttgacg
    10381 ccaacaatca gaaaaataag caaaacattg aaagaaagaa aaaaaatgtg atgcgtgaag
    10441 tacgggtgca ggggggggat gcatttgtta ttcgcagtgt ttccgttctt cctgaaagta
    10501 ttcccgctgg tttcagtgtt cttgcggggc ttgaggatga tgaatccctt ttgatcgtag
    10561 atctcggtgg tacaactctt gatgtgtcgc atgttcgtag caaaatgaca gggattacaa
    10621 aaacctggtg tgatccgaat atcggcgtgt ccctgattac atccggggtt aaggaacaga
    10681 tggctgtaca tgctaataca agagtgagct cattccaggc tgacaatatc attgtgcacc
    10741 gtaatgaacc tgactacctt tcccgtcgca tttataatgc tgaacagcgt gagtctatca
    10801 ttaatgttat caatgaacgg cagaagttgc ttattaaacg agttaatgat gtcatcagcc
    10861 gtttcaccga ctatacacat gtaatgtgtg ttggtggtgg ggctgaaatt gtggccgagg
    10921 ctgtgaagaa cctgactaag gttcctgatg agcgctttta tctgagctca tccccgcagt
    10981 ttgatttagt catgggaatg ataaaaatga aaggtggtgt gactaatgag tgatgaaaat
    11041 aaatccagac gctgctcatt tgaacttttc ccggatgaac ggacaggaga taagatagca
    11101 gatgagctca ttgcaaatga aaaactgaag gagcggggac gtttcatgcg cgcgatgtta
    11161 gttactggtg cggcgtttgc agctatagat aaacgtcttc ctttgctgat atcggagtta
    11221 ctgacagaaa atacgactct ggatgatatt aataaggtga tttccagcgt aataccaggt
    11281 gctttttcgg ttgaaaagaa attgcttgaa ttactggaaa aacaatctgg tctccatacg
    11341 tctgtggatt gcagtactcc tcttacagag cagagtctgt cgagaaatga cggtgaagac
    11401 cagaccagaa gaaacgctga aaatatgttc ggagatgact gaactaaccc agcctggcca
    11461 ggggaatatc tcgccattta gttgtataac actgactcaa catttcccgt ttcatttgcc
    11521 attcaggggc ggttcctcgc cccgcaaacc acactttccc ttttcccgac tggttcagtt
    11581 catcgagtgt tttcattaac ttttcgctgt ttttatgggg ctggatctca tcaaataatc
    11641 ccggctgcgc tatacccgat ggtgtgaaat cagccagcat gactcctgcc ttcatatagc
    11701 ggtagccttc acgccagacg tggtttaacg ctctgcatgc tgcggcaata atgtcccggc
    11761 tgtcctgtgt gggcaatgga agcttttcca cagcggaatt gctgtaacag ggggctttca
    11821 ctgcaaagag tgacgttcgt ataaatgtcg ccacctgccg gcaatactga cgctccccac
    11881 gtagtttctc tgctgcccgc tccgcatact gaacaacagc ctggtgtatg gcatctttgt
    11941 ctgtgattcg ttcaccaaaa ctgcgactac agacaatctg ctgttttgcc ggtggtgctt
    12001 cttccaggga tatgcaggac tcgccgttga gttcgcgtac cgtacgctca agaatgacgc
    12061 tgaagttttt ccggatgaat gccgtgttag cctgcgccag ctgcagtgct gtgttaatac
    12121 ccagcgcatt cagcttttct gtcagtctgc gtcctactcc ccagacttta ccaacaggct
    12181 gcagccccag taacttgaag atccgattac ggttttctgc cgtcagcgcg accataccgg
    12241 aaaactgtgg ccattgcttt gttgcccact gtgcactttt ggccagcgtt tttgtaggcg
    12301 caatgcccac ccccatggtg agtcctgccc agctctttac ctgttccctg agctgatgac
    12361 caaaaacttc cggagagatg caacggttca cctcccgcaa atcaatgaac atttcatcaa
    12421 ttgagtaggg ctcaactgcg ggagaaagcg actccagaac agccataacc cgttggctca
    12481 tgctgtggta cagcgcataa ttgctggaaa atacatgtat tttcttctcc aggtgcattt
    12541 gtctgacctg aaaccagggt tgccccattc tgataccaag ggcttttgtc tccgggctgc
    12601 gtgcgatcac acagccatcg ttattgctga gtacgatgac cggttcgttg cgaaggtccg
    12661 ggcggaaaac tttttcacat gaggcgtaga aactgttgat atcagccagt gcaaacatca
    12721 gcgtaactcc cgcgtcctgt gtatcacgtg agtgacaaca ccaaaaatac agatgttttc
    12781 cggatacagt gtgcggaact ccgggctgtc tgaaaccggc tccagtgctg ggcgtgggtg
    12841 caacagcagt cgtttgacgg taaactcccc gtcgatctca gcgataacga tgtccccgtg
    12901 ttgtggtttt tcggccctgt ccactaccag cagatcacca ttctgcacgc cagcctggtt
    12961 catcgattca ccgctggcgc gcagaaagaa ggtggctgca ggtctgctga tgcaatagct
    13021 gttcagatcc agttcctgct cagcataatc agtggcagga gaaggaaaac cggcctggca
    13081 acgatcggca aacaacggtc gcacgtaact gtcatcgcct gaagggtcag ccggacggtg
    13141 ataaacggta ctcatgctgc aggcagccag ctgtcgtcct gccagatttc ttccagcaga
    13201 gacaggatac gttctttatc cttgtcgttg cgggctcctg tgacagatac agacggggcg
    13261 ctgccttggt ctatacgcag ccagatttcc gggtagtgtg gtttcagacg ttgttcgatt
    13321 tcattctgta gcgcctgaag tgtgcctgat ttgaggtttt ttgtgctctg gcggtcgaaa
    13381 agaatttcaa tgcggatcat gttctcatct cccttttctt ttgctgtatg catatacagt
    13441 attctaatga ctgaatccac acacagtcaa atgtcattat tgtgactaaa aaacgctcaa
    13501 ttctctgagg gtgtggctgt atgcctgctg tggactgctg tgtccagctt atccacagca
    13561 ttttgtgcac ggttctgtgg acaaaatacc tggttaccta ggccgtgccg gcacgttaac
    13621 cgggctgcat ccgatgcaag tgtgtcgctg tcgccggcct tctcacccgg tcacgtttcg
    13681 tcgttcctcc tccacgcgct gcggcttcgg ggccgcacct gcattcgtat gcggtcgccc
    13741 ggttacaggt gcggcacggc ctgatggagg ccgcatgtga gaggagaatt cccatgccaa
    13801 actggtgcag taaccgtatg tatttttctg ccgaaccggc acagattact gagattaaac
    13861 gactggccag cggtgcagtt acaccacttt atcgccgcgc cacaaatgaa ggtattcagc
    13921 tgtttctggc cggaagtgcc ggacttctgc agaccactga agatgtgcgg tttgaaccat
    13981 gccccggact gacggttgcc ggacgtggtg ttgtatcgcc ggagaatatc gcgttcaccc
    14041 gctggctgac gcacctgcag aacggtgtgc tactggatga acaaaactgc ctgatgctgc
    14101 atgaactctg gcagcagagt ggtactggcc agcgtcgctg ggaaggatta ccggatgagg
    14161 tcagggaaaa catcaccgtg catttcaccg ccaaaagagg tgactggtgt ggcttctgga
    14221 gtaacgagga tgtatcggtg tggtggaacc gtctgtgtga caacgtactg ccggaaaaaa
    14281 ctatgccgtt tgacctgctg acggttctgc ccacccgcct ggatgttgaa gtgaatggct
    14341 ttaacggtgg tgttctgaac ggtgttcctt ctgcatatca ctggtatacg gaacagtatg
    14401 gcgtgaagtg gcctgtgggg tatgacctga atatcagcag tcagggagaa aacttcattc
    14461 aggtggattt cgatacgccg tggtgtcagc cggaaagcga cgttattgca gcattaagcc
    14521 gccgtttcag ttgcacgctg gagcactggt acgccgagca gggctgcaat ttctgtggct
    14581 ggcagcgata tgagcgtgga gagcttgttg atgtgctgtg gggggagctg gaatggtctt
    14641 ccccgacaga tgatgatgag ctgccggaag tcaccgcgcc ggagtggata gtcgacaagg
    14701 tggcgcatta cggtggatga agtatgacgg agacgggcgg gcaaccgccc gtttcttttc
    14761 cggtaaagga tgtcgccggg ctcctctttt tactgcgccg gctgacgcgg cgcggcagga
    14821 acgctgcctg tggtcagtgt cccgcgtccg gccgggcacg ggacgggcgc ttttaccgct
    14881 cccggctggc gtcggtgaca gtgtacgcca gcccgtcgcc cttttctgat gaacgtcctt
    14941 caagccgctt tcgcggcata ttctcgccgt caaacagacg acggctgcga tattccacgg
    15001 tcggcctgac ccgttaccgg acgtggtgaa cagcccacag gcagcgggga acgggcgcca
    15061 caggggcgcc ctcccggtgc cctctgtaag agaaggagtg ctgttatgtc ccgttttatc
    15121 ctgggtgact gcgtgcgtgt tatggccacg tttccgggca atgctgtcga tttcatcctc
    15181 accgacccgc catatctcgt cggtttccgt gaccgtcagg ggcgtactat cgccggcgat
    15241 aaaaccgatg aatggctgca accggcctgt aatgaaatgt accgcgtgct gaaaaaagac
    15301 gcgctgttgg tcagcttcta cggctggaac cgtattgacc gctttatggc ggcctggaaa
    15361 cgcgccggat tcagcgtggt agggcacctt gtattcacca aaaactacac atccaaatct
    15421 gcgtatgtcg cgtaccgtca cgaatgcgcc tatatcctgg caaaaggccg tccggcgctg
    15481 cctcagaagc cattacccga cgtgctgggc tggaaatatt caggcaatcg tcatcacccg
    15541 acggaaaagc ccgttaccag cctgcaacca ctgattgaga gcttcacaca cccgaacgca
    15601 attgtgctgg acccgtttgc aggcagcggc tcaacctgcg tcgccgccct ccagtccgga
    15661 cgccggtata tcggtatcga gctgcttgag cagtatcacc gtgccgggca gcaacgcctt
    15721 gccgccgtgc aacgggccat gcagcagggg gccgcgaatg atgactggtt tatgccggag
    15781 gctgcgtaaa tgaactatgc cggacacgaa aaacttcgcg ccgaagtggc ggaggtggcc
    15841 aatgccatgt gcgacctgcg tacaaccatg aatgagatgg agcggcggta cagctttaat
    15901 gctgacaccc tgccggaacg tctggtgcgt cagacgttgt ttcgcgcaaa ccacctcctg
    15961 atggaggcat ataccgaaat tctggaactg gatgcgtgct ttaaagattg agaaggagac
    16021 gagaatgtac ggaacatgcg aaacgctatg ccgggcgctg gcagcaaagt atccgggaga
    16081 cacgccgttg atgctggtta tctggtcccc ggaagagatt caggccctcg ctgacggaat
    16141 ggatatttcc ctgtccgatc atgaaatcag gaccgtcctg gcgcgcctgg aggacatccc
    16201 ggaagaccag cggactgaat ccggtatttc ttccggcgtg gcgatggaga tcatcaataa
    16261 cgtgagcgaa aaccgccagg tgaccttccc ggctgaactg ctggcgtccc tgattcagac
    16321 cgccgaacag gcactgtgga aacgtgaatg ggccgcccgt gattacggcc tcgccgtccc
    16381 ggaatgcgtt atccgccgtc aggcggtggt taatcaggcc cgcgccctgc tgaaaaacaa
    16441 cacacacgaa aacgactgat gttatcgccg cctccgggcg gcgattcagg gagaccatga
    16501 ataatgaata aaacgctgaa tgcactggtt tgtcgtcacg cccgtaacct gctgctggcg
    16561 cagggctggc cggaagagac ggatattgaa caactgaacc cgcactatcc ggactggatc
    16621 agcatttatg tgcggctgga tgcgccccgg ctggcgacgt tacttatcaa ccgccacgat
    16681 ggcgtgctgc tgccgatcct agcatctgcc gtgcaaaaaa tgaccggaac cggggcggaa
    16741 gttgtactgt ccggtagcca gtggcaggcg ctgccggtac ttccggcaga cggaacgcag
    16801 gtgtctttcc cgtatgctgg tgagtggctg gcagaagacg aaatcagggc tgtacttgcg
    16861 gcggtgcgcg atgcaatacg aagcatctgt taccaggtgg cagaagatgc acggggtatc
    16921 cgggcggcgc tgaccacaac cgggcagacg ttgctgaccc gccagacgcg ccgctttcgt
    16981 ctggtcgtga aggaaagcga ttacccctgc tggcttgatg aagatgacga aaatctgccc
    17041 gaggtgctca atgccatcct gaaccgtggc gcacgttttt cgtcggtgga aatgtacctg
    17101 gtcagtgagt gtgtggaaca tatcctggcc agtggcctgg tctgcgatgt gctgcgtata
    17161 ccggatgaac cgccccgccg ctggtttgac agggatattc tgcgggaggt ggtcctggag
    17221 gcacgggatg aaatccgcag catggcggat gccctggcaa aaatcagggg ctgatgatgg
    17281 cggggaaaat ccccgccttt ttcctgccgg tccccggaca gagcacaaag gaagtcgccg
    17341 ccggctccgc tttacccggc catgcggggc atggccttgt gggttttcag ttatgtggcc
    17401 gctgtgtcgt gtgcgggctg tgccgtgcct ccatcttagc caggctgaca gggatgcaag
    17461 ggtacgctcc gccgctgcgg tcacccggtc cctccttccc gtctgccgtg attttccggt
    17521 cgttcacccg ctcagatttc gcggtctcac cctccaactc ccgccagccg tgtgcgtcag
    17581 gctgcggctt cccttgcgcc ctgtgcatcc ccgccttatc gcccggcttt tatggaggca
    17641 cggcaccgcc cggtgtcgcg gaatatgtga ataacggagg aaaacatcat gcaatatgcg
    17701 aaacctgtca ctctgaatgt tgaagagtgc gaccgtctct cctttctgcc ttacctgttt
    17761 ggcaacgatt ttctgtatgc cgaggcgtat gtgtacgcgc tggcgaaaaa aatgatgccg
    17821 gagtatcagg gaggcttctg gcacttcatc cgcctgccgg acggtggcgg ttacatgatg
    17881 ccggatggcg accgtttcca catggtgaac ggtgcaaact ggtttgaccg taccgtgagt
    17941 gctgatgccg caggcatcat tctgacttcc cttgtgatta accgccagtt gtggctgtac
    18001 cacgacagcg gggatgcagg actgacccag ctttaccgga tgcgcgatgc gcagttgtgg
    18061 cgtcacatcg aatttcaccc cgaatgcaac gcgatttacg cggctctgga ctgattaacg
    18121 gacggggcgg caatgccgcc cctgtaaagg agacgagaac atgtactgca ctgttaaaga
    18181 aattatccgc gatgtactgg atacagacgt gccggacagt gaatgcgttt ttgccgtagt
    18241 gctgacccgt ggggatgtgc gtcacatagc ccaggactgg agtctgacag acgatgagct
    18301 ggaaaccgtc atgcagcggc tggacgatgc ctttgagcat ggtgcggatg tcagcgttgt
    18361 tcacgacgtt gttcgtgaac tgatggaaga aaagcgcgcc agccgtcatg tgacagtccc
    18421 ggcggtgatg ctggaaaaag tgatggcgct ggcaggcagt gaaatgaagc gcctgtatgc
    18481 cgtcgggagt gagaacgggg gcgacggtga cacattcgtc agggaagaac gcgaagcaat
    18541 ggacgttgtg ttacaggcgc tggacgggga gcacatgtca tgaatatcag cacagaaacc
    18601 cgcgaaattc tgcgcaatta caaagccgtg attaatgcgc ggcgtcgtga aatggggcag
    18661 aaaccgctca ccaccgcgca gattgttgat gaaatctgtg attttgtggc gaatcagcag
    18721 gctgttttcc tcggcagtca gtacatcctt cagggcggca gaaacaggtg atacaggggc
    18781 aggcggtgga ggccgtctgt cagcccgcgc gcccccctgg caggctgacg cctgccggaa
    18841 gggaagcggg ccacgctgac gcgtcgcccg ttacccttcc cgtttgtgat gtgtccggaa
    18901 tgtttttttc tgatgttatt tttctgtggt taccgtgcct gttattgttc ttcttcctct
    18961 tcttcttttc gttgctctcc tgtttatggc tttcccgggc gtcccgcttg cggggcgggc
    19021 tgtcgtgaac ggagccagcg gagctgtctc cgccctggcg ggcttccatc cctgacgcaa
    19081 aaccccgggc ttgcagcgac cggaacggtc gcgccaccct tgtcgcgccg tggcctgcgc
    19141 tcctggcatc ccggcaaaag gccgccggga tgtgaggcgc aacgccgttt cgcgcacgcg
    19201 ctctccgtcg ttgcggcggg gccctgctca ttgcctgccg ggtgccacac acccgtatga
    19261 gtccctgtcg ccgggggctc atacagccac aacctgccgc actgactgtc cagggtaaat
    19321 gaacagccca caaaattttt tacccaaaac ccggtaaaaa attttgcgtt ctgcccagga
    19381 caggtgcgtc aggtcgtggc agtgatgccc cttgcggcga aggttctcaa acgggtgaag
    19441 ttcataccac agggaataca gcagcaggtt cccgcgaaac gtcaaaaccg cggctcccga
    19501 aactcaacgg gtgatgttct ttaaaaatca ggatttattt cagactccgg caatacgcat
    19561 ttatttgttc tgtcagtcat ccggctggca ggtaatgaga acgcaggaac agaaggagga
    19621 aaacaccgga aatcgtcaga tgcagatatg aaaaaagcag gtcagggacc tgcttaatcc
    19681 atgccgcaga gactgcacga acagtttttg cggctccagt caatcgtcat aagaggctaa
    19741 attatgacaa taagtattgt ttcaccttcc gcagcggctg taaagccccg ccgtcatccc
    19801 cgtttcagac gcgaagacat ccccgcgccg gaaattgacc ccgtgctgaa agccttcggg
    19861 cgacacatag cccggagttt ttaccggggg cgtggtgtac acatccccgc catgaaaaac
    19921 acggccttcg ggcaggtgct ccgcacactc gaactgaaac gcgcttttaa ctgatgaggt
    19981 gagccgcccc gcgaggggcg gcaacagacg gaaggagaac attttatgac attagcagca
    20041 atcaccatga ccgccccgga agccgccagc cctgtgcaga tgtaccgcgc gacctactca
    20101 ccggatgaca acaaactgcg cctgtatgcc gcgtcacgtc ttgacccgga gacgtataag
    20161 aaagtacatg atgccggttt ccgctgggca ccaaaacagg cgctgtttgt cgcgccagcc
    20221 tggacaccgg gccgggaaga cgtgctcctc tcacttgccg gagagattga ggatgaagac
    20281 agcacgctcg ctgaacgtca ggaagcacgg gcggagcggt ttaccggata cagcggaaag
    20341 cgggccagtg aatccgcaca ggcacttgat gaagtggaaa gactggccgc gatgatcccg
    20401 cccggtcagc ccattcttgt ggggcatcac agcgaacgcc gcgcccgtcg tgatgcgcag
    20461 cgtattgaaa acggcatgaa acgtgccgtg atgctctttg aacgtgcgga atactgggaa
    20521 gagcgggcgc ggtcggcact gcttcacgcg aagtttaaag aacgtccgga cgttcgctgg
    20581 cgtcgcatca aaaaaatcga agctgatttg cgcaagtctg aaaagaacat cgcgcagtcg
    20641 cagaaatatc tgacgatgtg gcgggctgaa tcgctggatc tgaatatggc aaaactcatc
    20701 agcagtcatg accatatcag cgcctgtttc ccgctggata cgtatccgcg cccggcagaa
    20761 aaaagccagt atgaagggag ccggtcgtta tggtcggccc tggatgatga catcatcacc
    20821 acggagcagg cccgcgaaat tgcgatccgc tgtcatgaac ggcagattca gcatcagcaa
    20881 cgctgggtta accactatca gaaccgcctg atctatgagt gtgccatgct ggacgaaagc
    20941 ggcagcgtgg ttacccggac acaggatttt gagccgggcg gacaggtttt cagccggggc
    21001 gagtggctga ccatcatacg cgtgaacaaa agcaacgggg cggtgagttc agtcacaacg
    21061 ccgaattaca gttttctcgg gtacagcggc acgatgaaag tgacgcccga tcgcatcacg
    21121 gactacaaag caccatcggc agaagaggct gccgtcgcca gccaggccgc gaagcgtccg
    21181 ccggtagtca actatccggg ggaaggtttc cgggaaatga caaaagcaca gtgggccgcc
    21241 ctgccccggg actgtaaggc cgtgcgcagt gtggcagaag cagaagacca cggggcatac
    21301 cgctaccgcc gcacaatgga caataatttc cgtctggtga atgtgtatat caccgacatg
    21361 aaaattacgg aaatcccaca gaaataaggt atatcccccc gggaaatccc cgggggatga
    21421 cggagataat gacatgcaca gtcagttaaa agaacgtatc cggctgatgc gcgcaaggct
    21481 ggataacgcc gcgccggttg ctgaaatccg ggctgaatct cagctttttg tgactcctgc
    21541 cccggtctgt gatcgcctgg tgacgctggc ggagatcagc aaccgtgatc acattctgga
    21601 accctctgcc ggcaccgggg ccatactgcg ggcgattcgg gataccgcgc cgggggccat
    21661 gtgtgatgcg gtggaaatca acagcgggct ggtccggtat ctgcgggaaa attttaacgg
    21721 tgtcagggtt cagtgtggcg acttcatgga atggcagccg gtgcagtatt acagccgggt
    21781 tatcatgaat ccgccgttca gccacgggca ggatatccgg catatccttc gggccttttc
    21841 cctgttgcgt ccgggtggtg tgctggttgc cgtctgtctg aacgggccgc gccagcagga
    21901 gaagctgtta ccgttttctg acgtccgcga ggagctgccg cgcggcacgt ttgcttatac
    21961 cagtgttccg acgatgatta ttcgtctgcg tgcctgatga ctccgggcag gcggggggat
    22021 aaatgcccgt ctgcccgcgc cgggaaaaga ccgcgcgccg gtggcgctga aaggggccgc
    22081 gctcacgcgc cgcccctttc attacgcgga cgttctgctg tgggcttctg tcttttcaga
    22141 cctgtcattt gcctgttact gatgatgttg ttgttcctgc tactgctact gctactgcta
    22201 ctgctactgt tcttcttttt tctgctgctc tgattactgt ctgttttgtt gttctcctgc
    22261 ttatgccttt tccgggcgtc cccctggcgg ggcgggctgt cgtgaacgga gccagcgaag
    22321 ctgtctccgc cctggcgggc ttccatccct gacgcgaaac cctggcttgc agcgaccgtt
    22381 ccggtcgcgc cgcctctgtc gcgccgtggt ctgcgatcct ggcatcccgg caaaagaccg
    22441 ccgggatgta aggcgcaacg tcgtttcgca aaccgcgctt cacgtcgttg cggcgggtgt
    22501 ctgcccgttg ttgtgtccgg tgccctacac ctgtttaagc ccctgtcgca gggggctgat
    22561 acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca aaatttttta
    22621 cccaaaactc ggtaaaaaat tttgcgctct gccccggaca ggtgcgtcag gccgtggcag
    22681 tgatgcccct tgcggcgaag gttctcaaac aggtgatggt taataccgga ggaaacaaaa
    22741 ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat gatgggggct
    22801 gaaaacatca acagaaggag acacatcatg gcagttcgtg gcattaacaa ggtcattctc
    22861 gtaggacgcc tgggaaacga tccggaagtc cgttacatcc ccaacggggg cgcagtggca
    22921 aacctgcagg tggccacgtc agaaagctgg cgtgacaaac agacggggga gatgcgggag
    22981 cagacagaat ggcatcgcgt ggtgttgttc ggcaagctcg cggaagtggc aggtgaatat
    23041 ctgcgcaagg gcgcacaggt ctacatcgag gggcaactcc gtacccgtag ctgggaagat
    23101 aacggtatca cccgttacgt cactgaaatt cttgttaaga ccacgggcac catgcagatg
    23161 ctgggacgtg ccgcaggtgc tcagactcag ccggaagagg cgcaacagtt cagcggtcag
    23221 cctcagccgg aaccactggc ggaggccggt acgaaaaaag gaggcgcaaa aacgaaaggc
    23281 cgtggacgta aggccgcaca gccggagcct cagccgcaac cgccggaggg tgacgattat
    23341 ggtttttcag acgatatccc gttctgaacg actgactgtg acaaccgccc cgtcctgtgc
    23401 ggggcatcac cggagagatg aggatgagcg aatatttcag aatacttcag gggctgccgg
    23461 acggctcctt tacccgcgaa caggcggaag ccgttgccgc acagtactgg aacgtcttta
    23521 tcgaggatga ccacggggaa cattttcgcc tggttgtccg caataacggt gcactggtct
    23581 ggcgtacctg gaattttgag gacggtgccg ggtactggat gaaccatgtc atccgggatt
    23641 tcggcattct taagtaagag aggtgccgga cgggaacgcg tccggcagac atacaaccat
    23701 cccggtcaaa ggagaaatat atgtcagtta cagagtctaa gacaaaaacg gagcgtaaat
    23761 ccagccgtaa acctgcaaaa aagcaggaaa cagtcctgtc tgccctgctg gcgcagacgg
    23821 aggaagtgag cgtgccgctg gcctcactga ttaaatcacc gctgaatgtg cgcacggtgc
    23881 cgtattctgc ggagtccgtc agcgaactgg ctgagtccat taagggcgtt ggtctgttgc
    23941 agaatctggt tgtgcatgtc ctgtctggtg accgttatgg cgttgccgca ggtgggcgca
    24001 ggctggcggc actcaacatg ctggcagagc gcaacatcct tccggctgac tggcctgtcc
    24061 gcgtgaaggt tatcccgcag gaactggcga ctgccgcatc aatgactgaa aacggtcatc
    24121 gccgggatat gcaccctgcc gaacagattg ccggattccg cgcaatggcg caggaaggca
    24181 aaacatctgc acaaatcggt gatttgctgg gttattcgcc ccgccacgtt cagcgaatgc
    24241 tgaaactggc agaccttgcg cctgtcatcc tcgatgcgct ggcagaagac cgcatcaaca
    24301 cagagcactg tcaggtgctg gcgctggaga acgacaccgc gcgtcaggtg ctggtgtttg
    24361 aagctgcctg tcagtcggga tggggcggta aaccggaagt acagaccatt cgtcgtatgg
    24421 tgaccgaaag tgaagtggcg gtggcgggga acagtaaatt ccgcttcgtg ggggctgatg
    24481 ccttctcgcc agatgaactg cgcaccgatt tgttcagcga cgacgagggt ggctatgtgg
    24541 actgcgtggc gctcgatgcc gccctgctgg aaaaactcca ggctgtcgct gaacacattc
    24601 gggaagccga aggctgggaa tggtgcgccg gacgtatgga gcctgtcggt ttctgccgtg
    24661 aggatgccgg aacataccgt tgtctgccgg agccggaggc ggtgctgacc gaggcggaag
    24721 aagaacgcct gaacgaactg atggcacgtt acgacgcgct ggaaaaccag tgtgaggaat
    24781 ccgacctgct ggaagcagaa atgaagctga ttgactgcat ggcgaaggtc atggcgtgga
    24841 cgccggagat gcgtgccgga agcggtgtgg tggtgtcctg gcgttatggc aacgtatgtg
    24901 tccagcgtgg tgtgcagttg cgcagcgagg atgacgcggc tgccgacgct gaccgcacgg
    24961 aacaggtgca ggagaaagcg tcagtggagg aaatcagtct gccactgctg acgaaaatgt
    25021 cttccgaacg cacgctggca gtccaggcgg cgcttatgca gcaaccggac aaatctctgg
    25081 cactgctggc atggacgctc tgcctgaatg tgtttggcag cggagcgtac agtaaaccag
    25141 cacaaatcag cctggaatgt aaacattatt cgctgaccag cgatgcgcca tcggggaagg
    25201 aaggtgccgc attcatggcg atgatggcag aaaaagcccg tcttgcagcc ctgctgccgg
    25261 agggatggtc acgggacatg acgacgttcc tgtcactcag tcaggaggtg ctgttatccc
    25321 tgctcagttt ctgcaccgcg tgcagcatcc acggcgttca gacccgcgag tgtggtcaca
    25381 cgtcacgcag tccgcttgac tcgctggaaa ccgctatcgg ctttcacatg cgcgactggt
    25441 ggcagccgac gaaagcaaac ttcttcggac acctgaaaaa gccgcagatt atcgcagccc
    25501 tgaatgatgc cggactgtcc ggtgccgcac gggacgcgga gaagatgaag aaaggcgatg
    25561 ccgctgaaca tgcagagttc cacatgaaag acaaccgctg ggtgcctggc tggatgggtt
    25621 caccacgtcc acagacggat gccactgaac gcgccgataa cctggctgat gccgcctgat
    25681 gaacaaccac accgccccgc cggagacggg gcggcagcaa gggagatacc gtgatgaaaa
    25741 ctgaactgac cctgaatgta ttacagacca tgaacgcaca ggaatatgaa gatatccggg
    25801 ctgccggaag taatgaacgc cgtgagctga cacatgccgt gatgcgggag ctggatgcgt
    25861 cggacaactg gacgatgaac ggcgagtatg gcagtgagtt cgggggcttt ttccccgtcc
    25921 aggtccgttt cacgccagcc cacgaacgtt ttcacctggc gttatgttca ccgggtgacg
    25981 tctctcaggt ctgggtgctg gttctggtga atgccggtgg tgagccgttt gcggtggttc
    26041 aggtgcagcg gcggtttgca ccggaagccg tcagccattc actggcactg gcggcgtcac
    26101 tggatgcgca ggggtacagc gttaacgaca tcatccatat cctgatggcg gaaggaggtc
    26161 aggtatgagc gcatgttcac aggcactggt tcccctcagc acagagcaac aggccgcatg
    26221 gcgggcggtg gctgagacgg aaaaacgccg ccatcaggga aacacgctgg cggaatatcc
    26281 gtatgcaggg gcgtttttcc gctgtctgaa cggaagccgc aggatatcgc tgtctgacct
    26341 gcgatttttc atgccgtccc tgaccgcaga agaactgcac ggcaaccgtc ctcagtggct
    26401 gtatgccatc gatgtgctga ttgaaacaca gggcgaggtc tgcctccttc cccttcccgg
    26461 tgatgcagca gagcggttgt tcccgtcggt gcggttccgt gtcagggaac gcagtcgcca
    26521 taaatccgtg ctggtcatgc agaagtacag ccgccagcag gcacgggagg cagagcagaa
    26581 agcccgtgcg taccagacgc tggtcgctca ggctgaaatc gaactggcgt tccattcacc
    26641 ggaaacagtc ggaagctggc acgcccgctg gagtgaccgg gtggcagagc atgacctgga
    26701 aaccctgttc tggcagtggg gtgagcgttt tccgtcactg gccgggatgg aacgctggca
    26761 gtggcaggat atgccattct ggcaggttat cgcagaggca ggtatggcag ccagagaggc
    26821 cggtcatgct gtccgtgaga tggagcggtg gatggtgccg aacaaactcc gggaggcagc
    26881 atgatacggc aacaatcaca cggattgccc gtaaacagcc tgaatgagcg ggttattttc
    26941 aggaaaagtg aatgtggtca gagcgcaggg atctgggcta tgatgtgccc ggcgcttgag
    27001 gctttctgcc tcatgacgtg aaggtggttt gttaccgtgt tgtgtggcag aaggacaaaa
    27061 gccccgtagt taattttcat taacccacga ggcccctgca tgcttagaca acatcaggat
    27121 agcctcttac cgcgctttgc gcaaggagaa gaaggccatg aaactaccac gcagctccct
    27181 tgtctggtgt gtgttgatcg tgtgtctcac actgttgata ttcacttatc tgacacgaaa
    27241 atcgctgtgc gagattcgtt acagagacgg atacagggag gtggcggctt tcatggctta
    27301 cgaatccggt aagtagcaac ctggaggcgg gcgcaggccc gccttttcag ggctgatgca
    27361 ggtctgactg cactgaagcg cctttataaa ggggctgctg gttcgccggt ggcccctttc
    27421 tccttgctgg cattgtacgg gcatgaacaa ccagactgcc aggaaggctg tttccctccg
    27481 ggaacgggga tgaacgtgcg cctgcggcgc ccgttctttc cccccgcctt ctctgtttat
    27541 gacctgtcag aaatcacatc accttccctt tgattattct ctttttcctc ctgttctgac
    27601 tctgattact gctgccctga ttattgtctg ttttgttgtt ctcctgctta tacctttgcc
    27661 gggcgtcccc ctggcggggc gtgctgtcgt gaacggagcc agcgaagctg tctccgccct
    27721 ggcgggcttc catccctgac gcgaaaccct ggcttgcagc gaccgttccg gtcgcgccgc
    27781 ctctgtcgcg ccgtggcctg cgctcccggc atcccggcaa aaggccgacg ggatgtaagg
    27841 cgcaacgccg tttcgcaaac cgcgcttcac gtcgttgcgg cgggtcccga aactcaaaag
    27901 atgatggggg ctgaaaacca gagagaggag aaagcaggat gagtacccga aatatccacg
    27961 ttaacaccgc gtcgtacacg cttcttgttg ccgggaagaa aaaaaacacc ggggaagagt
    28021 gggacgtcct ggaattcagc agcctgactg agctgaaaaa atatcgcaaa agccacccgg
    28081 aaaagatggc cttcagctat aactacgcgc tcagccaggg tgtggatacg cagttccgcc
    28141 atatcaacat tgcggaagct gatcacttca aacagttcct gcgtcagatt aagcgtgccg
    28201 gcctcgatat ccgggcgctc tgctgaccgg atgtgatgaa tatcccttct gattgttata
    28261 atgttgttaa tggagacggg agggatgata ttaagcagaa aaatttttta ttaaatttat
    28321 gctttcataa aggagtagct gttatgcgat tagcttcccg ttttggtcgg tataattcca
    28381 tccgccgtga acgtccttta acggatgatg aattaatgca gttcgtgcct tcggtatttt
    28441 ccggtgataa acatgagtcc cggagtgaac gttatacgta tattccaaca atcaatatca
    28501 tcaataagtt acgtgatgaa ggtttccagc cattctttgc ctgtcagagt cgggttcgtg
    28561 atttggggcg tcgcgaatac agtaaacata tgttacgtct tcgcagagaa gggcatatta
    28621 acgggcagga agttcctgaa attatcctgc ttaattcaca tgatggttca tccagttatc
    28681 agatgatctc cggaattttt cgttttgtct gcacaaatgg cctggtgtgc ggaaataatt
    28741 ttggcgaaat ccgcgttcca cataaaggtg atattgtcgg gcaggttatc gagggagcgt
    28801 atgaagtgct cggtgtcttt gataaggtca ctgataatat ggaggcgatg aaagaaattc
    28861 atcttaacag tgacgagcaa catttatttg gcagagctgc actgatggtc aggtatgaag
    28921 atgaaaataa aacgccagtg acgcctgaac aaataattac tccccgtcgt cgggaggata
    28981 aacagaacga tctctggaca acctggcagc gggttcagga gaatatgata aaaggtggat
    29041 tatcggggcg aagtgcctcc gggaaaaata ccaggacaag agccattaca ggtattgatg
    29101 gtgatataag aatcaacaag gcgttgttgg tgattgccga acagttcaga aagtggaagt
    29161 catgataata taagtaaagc cccgaaaaat tttcggggct ttacttatat tatcattctt
    29221 cggcatcgct gtgtcgtctg atatacctca atttcatcaa ctggctttcc ggttgtctgt
    29281 aaatattgtg cccgcgaaaa atggtctggc aggaggctgc gcagttaccg ggtatatata
    29341 agatgggttc ccgcttcgcg gggcttcggc cagtcagtat tgtcactcat atgatatttt
    29401 tgtgtgtggc cttccatgcc gcttttgcgg cataaccggt atctgacaat gtctgtaaga
    29461 ttaattgctt tgcacgctgt taatttccgt aagtgatttc ttcgtagccg gaatccggat
    29521 acctttactg ctttttatcc cggtataaat ccggtaaaca tctgatgcgt aggcaagacg
    29581 tctctggttc tggcgttcgg tcttcctgaa tccggcattg tatgcaccaa cggcctccca
    29641 ggagacgccc cattttttaa aggctattgc cagataataa gcaccggtat aaatgttcat
    29701 gcagggatct gttgtcagat gttccggatt aattccatag cgggccagtt cgttaaaatg
    29761 ctgggaatct acctgcatca gtccgctgcc atatcccgtt accggattaa taccgatggc
    29821 attaacccgg taacgggatt ctttccatga tattgctctc agtaaatcag gatctatttt
    29881 gtaatccctg cctgcaagat caaagcaatc agtggcctgg cagatctcat ttataaacat
    29941 caggcagatg gctaacatcc attttttcat ttttccacct ctggtgactt tatccgtaaa
    30001 taatttaacc cactccacaa aaaggctcaa caggttggtg gttctcacca ccaaaagcac
    30061 cacaccccac gcaaaaacaa gtttttgccg atttgttttt taaatcatta gtttatgttc
    30121 caaataatgt attttaattt attttacatt attaaatagg gtcgttgacg accctatggc
    30181 gacccttgtt agggtcagtg ctgacaattt tcatgaaaaa catttggata tattagggcc
    30241 gcccctagca gcgccgtgcg tttttttata gtatgcccct aggggcgctg ctaggggttc
    30301 tcttcttttt tcagaccata ctatgagtag atgaaataaa actcttattt cagaatctaa
    30361 aaggtactgt cttatggcca gagtaaatct gtatatcagt aatgaggttc atgaaaaaat
    30421 taacatgatt gttgaaaagc gtcgtcagga gggagcaaga gataaagata taagcctttc
    30481 aggaactgct tcaatgcttc ttgaattagg gctccgtgta tatgacgctc agatggagcg
    30541 taaagagtca gcgttcaatc aggctgagtt taataaggtg cttcttgaat gtgcggtaaa
    30601 aacacagtca acagttgcta agattttagg tattgagtca ctaagccctc atgtttccgg
    30661 gaatccaaag tttgaatatg cgaatatggt cgaagatatc agggagaagg tgtcatctga
    30721 gatggagagg ttctttccag aaaatgatga ggattaaaaa agaattaaaa gttgatttgc
    30781 ttcaaataac aggattttga tctggttcaa tttgatgaca attaaccgca taaaggttat
    30841 attaattatg tggttaatgc cacgttaaaa cttgatatta aaaatcgccg atgcagggag
    30901 gtaagagctc cctgcatcga ctgtccatag aatccttaac ggaggttcct atgtgtgcac
    30961 tggatcctag agaaaggcca cttaacagtc aatctgtaaa taaatacatt cttaacgttc
    31021 aaaatatcta caggaattct cctgttcctg tttgtgtccg taacaaaaac aggaaaatcc
    31081 tttatgccaa tggggccttt attgaactct tttccaaaga agataaaccc ttctctggtg
    31141 aaagttatgt acgtctgcag gttgaaattt ttctttcatc acttgagctt gaatgccagt
    31201 ctcttgggca tggttccgca ttttgtcgtc gttttaattt tcatggagaa atctatcaaa
    31261 taaggatgga aaatgtttca ttttataatg aagaatctgt tgttctatgg caaattaata
    31321 tattccctga ctatccattc tttcgtgtgg aaaaagaaaa ttattatcat cgtgatagct
    31381 atgtacagtc tgttatatcg aatatgacag caaaatcatt ggttgttttt tgtttctatg
    31441 cattagggta taaacatata aatatagcta aagaattaaa aatcacagaa gtggcttcta
    31501 aaaaaaggat taaaaagatt aatgcggata tcaataaaag attgggttcc ttcgatttgt
    31561 tcaggaataa atgtattaag acaggtgtaa tgtacgaaat attaaacgtt ttgagtgaat
    31621 atatgggtgt taaaaaaatg taataaagca gggctcgttt tgggcccatt ttcgacaggc
    31681 catttctgtg caatcatgat tttattggtc aggaaacaga ggtgaattga gtgaggaggc
    31741 gtaacgcgag aggcgggata agcagaacag tctcagtgta tctcgatgaa gatacaaata
    31801 accgacttat tcgggccaaa gatcgtagcg gtagaagtaa aactatagag gttcagatcc
    31861 gtctcagaga tcatctgaaa cggtttcctg atttctataa tgaagaaata ttcagggagg
    31921 tgatcgaaga gaacgagtca acatttaaag aactttagtt tttatctatc aatattaaac
    31981 agaggaatca tatgaatact gttttaagtg ttcagggtgc ttctgcgccc gttgaaaaga
    32041 aatcgttttt ttctaaattc actcgtctga atatgcttcg cctggctcgc gctgtgatcc
    32101 cggctgctgt tctgatgatg ttcttcccgc aactggcaat ggcagcacag ggccaggacc
    32161 tgatggcaag cggtaacacc acggttaagg cgaccttcgg taaggactcc agtgttgtta
    32221 aatgggttgt tctggctgaa gttctggtcg gtgcggtcat gtacatgatg accaaaaacg
    32281 tcaagttcct ggccggtttt gccatcatct ctgtatttat tgctgtgggt atggccgtcg
    32341 ttggcctctg acaggaaata aaacgatgtc gggagacgaa aataaactta agaaatatcg
    32401 tttcccggaa acactgacca accagagccg ctggtttggc ctgccactgg atgaactgat
    32461 ccccgcagca atctgtattg gctggggtat cacaacatca aaatatctgt tcggtattgg
    32521 cgcagcggtt ctggtttatt tcgggattaa aaaactgaaa aaagggcggg gcagttcctg
    32581 gttacgtgac ctgatttact ggtatatgcc aacagccctg ctgcgcggta tttttcataa
    32641 tgttcccgat tcgtgtttcc ggcagtggat taaatagaac tgataccagg attgttatat
    32701 ggaacacggt gcccgtttaa gtaccagtcg tgtaatggcc atcgccttta tatttatgtc
    32761 agtgcttatt gttctcagcc tctctgttaa cgtcattcag ggggtgaata actaccgtct
    32821 tcagaatgag caacgcactg ccgtgacgcc aatggcattt aatgccccct ttgccgtgtc
    32881 acagaacagt gccgatgcct cttatttaca gcagatggcg ctgtcattta ttgccctccg
    32941 tctgaatgtt tcatcagaaa ccgtcgatgc ctcacatcag gcgcttctgc aatatatccg
    33001 cccgggcgca cagaaccaga tgaaagttat tctggctgaa gaagcgaagc gtattaaaaa
    33061 cgataacgtg aactcagcct ttttccagac cagtgttcgt gtctggcctc agtatggccg
    33121 tgtggaaatt cgtggtgtgc ttaaaacctg gattggtgat tcaaaacctt tcactgatat
    33181 caaacattac atccttattc tgaagcggga aaacggggtg acatggctgg ataatttcgg
    33241 ggaaacagac gatgagaaaa aataatacag caataatatt cggcggcctg tttttttcct
    33301 gcagcgtgat ggccggagcc ggtacgctgg cccccaccgt ggtgccaatg gtgaatggtg
    33361 gtcaggccag tattgccatc agcaatacca gcccgaatct gtttaccgtt cccggtgacc
    33421 ggattatcgc cgtgaacagt ctggatggtg ccctgaccaa taatgagcag accgcctccg
    33481 gcggtgtggt ggtggccacc gtcaacaaaa agccctttac gttcattctg gaaacagaac
    33541 gtggtctgaa tctttccatt caggccgttc cccgtgaagg cgcggggcgt accattcagc
    33601 tggtcagtga cctgcgcgga accggagaag aagccggtgc gtgggaaacg tccacgcctt
    33661 acgaatccct gcttgtgacc atcagccagg ccgtccgtgg cggaaaatta cccgcaggct
    33721 ggtatcaggt cccggtgaca aaggaaaccc tgcaggcccc ggcggggctg tcttcagtgg
    33781 cagatgccgt atggacgggg aatcacctga agatggtccg ctttaccgtg gaaaataaaa
    33841 cgctgtctgc cctgaatatc cgggaaagtg atttctggca gccgggaacc cgtgccgtga
    33901 tgttcagcca gcctgccggc cagttactgg caggtgcgcg catggatgtg tatgtcatcc
    33961 gtgacgggga gagcaactga tggccagtat caataccatt gtgaaacgca agcagtacct
    34021 gtggctgggg attgtggttg tcggtgcagc ctccgcgatt ggtggtgcac tgtatctgtc
    34081 tgatgtggac atgtccggta acggtgaggc ggtggctgaa caggagcctg tgccggatat
    34141 gaccggtgtg gtggatacga cctttgatga caaggtgcgt cagcatgcca ccacagagat
    34201 gcaggtgacg gcagcgcaga tgcagaagca gtatgaggaa atccgtcgtg agctggatgt
    34261 tctgaacaaa cagcgcggtg atgaccagcg tcgtattgaa aagctgggac aggacaatgc
    34321 cgccctggct gagcaggtaa aagccctggg tgctaatccc gtcacggcga ccggtgagcc
    34381 tgtaccgcag atgcctgcct caccgcccgg cccggaaggc gaaccacagc caggaaacac
    34441 ccccgtatcc ttcccgccgc agggcagcgt tgctgttcca ccgccgacgg cgttttatcc
    34501 cgggaatggt gtcacgccac caccacaggt gacgtaccag tctgtgccgg tgcctaaccg
    34561 gatacagcgt aaggtgttta cccgtaatga gggaaaacag ggaccatcac tgccgtacat
    34621 tccgtcagga agttttgcga aagccatgct gattgaaggg gcggatgcca atgcctctgt
    34681 caccggtaat gaatccacgg tgccgatgca gctgcgtatc accggcctgg tggaaatgcc
    34741 gaacagcaag acgtatgacg caacgggatg ttttgtgggt ctggaagcct ggggggatgt
    34801 gtccagtgag cgtgccattg tacgcacccg caatatcagt tgcctgaagg acggcaaaac
    34861 tattgatatg ccgattaagg ggcatgtcag cttccggggt aaaaacggta tcaagggcga
    34921 agtggtgatg cgtaacggca aaatcctcgg ctgggcatgg ggcgcgggat ttgttgacgg
    34981 tatcggtcag ggaatggagc gcgcctccca gccggctgtc gggctgggtg ccacagccgc
    35041 ttacggggct ggtgatgtcc tgaaaatggg tatcggtggc ggcgcatcga aagccgcaca
    35101 gacgctcagt gactactaca tcaaacgtgc cgaacagtat cacccggtga taccgattgg
    35161 tgcgggcaac gaagtgaccg tggtgttcca ggacggcttc cagctgaaaa ccgtggaaga
    35221 gatggcgctg gaacgcacgc agagcagagc ggaagaagac aatccggaaa gtccggttcc
    35281 tgttccgcca tcggctgaaa gtcatcttaa cggctttaat actgaccaga tgctgaagca
    35341 gctgggcaac ctgaatccgc agcagtttat gtccggcagc cagggagggg gcaacgatgg
    35401 caaataatat gtcgtcccgt caggcgtgcc atgccgcccg gtatgttgtc gcccgggttc
    35461 tgcgaggact gttctggtgt ctgaagtaca ccgtcattct gccactggcc acaatggccc
    35521 tgatggctct gtttgtttta tggaaggaca acaccacgcc ggggaaatta ctggttaagg
    35581 agattaactt cgtaagacaa acagcacctg ccggacagtt cccggtcagt gaatgctggt
    35641 tttcttcctc tgattcttca ggcagatcag aaatacagga tatctgccat taccgcgcgg
    35701 ctgatgctgc agattatgtc agggagactg accggtcact gatgcaactg gtcaccgcat
    35761 tctgggcaac gctggcactt atgtatatgt cgtttgcggc aggcaccggg aaatatccga
    35821 tgtggcccgg aaaaatgaaa tgtgtccggg tgggaaccgc agatgaacat ctgaaggaag
    35881 tttatacgga agatacttct ctgtcggaaa aaatcaggaa atgccatgtt tatttccctg
    35941 atgacagggc aaacagaaac aacggagata aaaatgaaca tgcgtaatat taatattatt
    36001 actgcacgct ctgtaccgga taaaagcgta tcagatgatt ttatgaatgc tgttctcagt
    36061 aactgcacca caaaaatagt tcttcctgct ccgaaggtgt tcagttctga atcattgccg
    36121 cacaatttta atatggctgc tgtcggtgtg atgaagaagg gaatgtcgat gagattattt
    36181 tcttctccgg gaaatacagt ggtctccgga catccctgtg cagggaaaac aaatctgaca
    36241 gcagagttaa tttctgctgt tgtggggagt aataaaaaag gagaagggca atgaaacaga
    36301 tttctttatt tattcctctg ctgggaacct tattactttc cggctgcgca ggaacaaata
    36361 cggaatttga gtgtaacgcc accacatccg atacctgtat gacgatggag caggccaatg
    36421 agaaggccaa aaaactggag cagtcctcag aagcaaagcc ggttgcggca tcactgccac
    36481 gcctggctga agggaacttc cggacaatgc cggtgcagac cgtcaccgcg accactccgt
    36541 ccggcagcag acctgcggtg acagcacatc cggagcagaa actgctggca ccacgcccgc
    36601 tgtttaccgc tgcccgggaa gtgaaaacgg ttgttcaggt cagttcagtt gtgccggtaa
    36661 cacctccccg tccgctaaga acgggtgagc agacggctgc attatggata gcgccttata
    36721 ttgataatca ggatgtttat catcagccat ccagcgtgtt ttttgttatt aaaccgtctg
    36781 cgtggggaaa accacgtatt aattaactgg ccctgaagtt gtatatcagc cggcaatgcc
    36841 ggcgggcttt ctgtattcaa aatctgcctg taaaacgagt gccctgtgcg tgaaaaggga
    36901 tgggcggtag cgtgtcagaa catgtcggga ggaatattcc gtgagtgatg aagccgatga
    36961 agcatattca gtgacagaac aactgaccat gacaggaata aaccggatac gccagaaaat
    37021 aaatgctcat ggtattcctg tttatctctg tgaagcatgc ggaaatccta ttccggaagc
    37081 ccggcggaaa atatttcccg gtgtgacgtt atgtgttgaa tgtcaggcgt atcaggaaag
    37141 acagaggaaa cactatgcat aaatcagaaa atgaaaaaat agattttatt ctggatgagc
    37201 atgagcggat attcagaaaa cagggttctt tgtgtatggc actgggtaag ggctttttag
    37261 ttatagctat tctttgtggt gttgccacat tctgtgtttc tggcctatat ctgaaatccc
    37321 tgtgcctgtc cgttgcctgc tgttgtgggg tattcagccg tatttttaat tattacagcg
    37381 taccggagga atcacgcagg gtactcagtc gtcaggaatt gctctggctt atgagtctga
    37441 ctgaagactg tccggatatg caccaggaac tgcttaactg cctgctgtcc ggtaaaaaac
    37501 tgaccggtct ggacaagagg gaaatcagga gtctctggtg ggaaaagatg gatgcaatgc
    37561 aggagtctgc cactcgtcag cgtgaacagg ataccattcg gcagttttct gaagagagta
    37621 aatccgaatg aaaaatctgc ggtgtttctg acctcatagt taactgaatg aatattcagt
    37681 gattaatatt taactggagg taataaagaa tgcatacctt tatttttctt gtgctgataa
    37741 aaaatattaa cggtggcgga agctggaatg ttacccccat gccaaatacg gacgtatgcg
    37801 tgcaggttct tcggacaatc gaagcacatg gcggcagggg ctttggaaat acaggggagt
    37861 gtattgaagt taaaacaaat gaacctttga acagcgtgat ggaaacacta aatggtggtg
    37921 ggcaatgagt gcttactgct tcgaaggaaa atgaaatgaa gtcgatatat ttaaaatctg
    37981 ttctggcttt tatatttgtt ggcgttatgg ctatgttgat atgtggtctt ttttacaatg
    38041 attaccttga acaacaacct gcaacaccag aacaactgac agagatttta caggaaactc
    38101 cctgtgcagc agaagcattt caggagacac ttaattatca gtctgaacct ttaacacttg
    38161 gtaaggcaaa taaaatcgca tcagaatgca gaaaaagaaa cgaaatggca gaggcgcgac
    38221 gggtaagaga aaacgagctc aataagatac gcgaaaaaca acttcaggca ttaagtgatg
    38281 ctgattcagc aaaggaacgt taattgtaat cctggtttcc tgttaaaagc ctccatctct
    38341 tatttgtgct gtggtttcag aaatggcact gcgtgatata tccacgccac agactgatat
    38401 tttccttttt taaacgcact aaggaatact catatgagta ataacccact tgaggccgtc
    38461 actcaggcgg ttaactctct cgtcacagca ctgaaactgc ctgacgagtc cgcaaaggcc
    38521 aatgaaatac tgggtgaaat gagtttcccg cagttcagcc gtctgctgcc gtaccgtgat
    38581 tacaaccagg aatccggtct gttcatgaat gacaccacgc tgggctttat gctggaagcc
    38641 attcccatta atggggcgaa tgagtccatt gtggaggcgc tcgaccacat gctgcgtacc
    38701 aaactgccgc gcggtattcc gttgtgtatc catctgatgt ccagtcagct ggttggtgac
    38761 aggattgaat acgggctgcg tgagttctcc tggtcaggtg aacaggccga acggtttaac
    38821 gccattaccc gggcctatta tatgaaagcg gcagcgacac agtttccgct gccggaggga
    38881 atgaatctgc ccctgaccct gcgccattac cgggtgttta tctcgtactg ttctccctcg
    38941 aagaaaaaaa gccgggccga cattctggaa atggaaaacc tggtgaaaat catccgggcg
    39001 tcgttacagg gggccagtat caccacacag acggtggatg cacaggcctt tatcgatatt
    39061 gtcggggaga tgattaacca taacccggac tccctgtacc cgaaaagacg tcagctggac
    39121 ccgtattctg atctgaatta tcagtgtgtg gaggacagtt ttgatctgaa agtccgggct
    39181 gattacctga cgctgggcct gcgtgaaaac ggcaggaaca gcacggcccg catcctgaat
    39241 ttccatctgg cccgtaaccc ggaaatcgcc ttcctgtgga acatggccga caactacagc
    39301 aacctgctga acccggaact gtccatctcc tgtccgttca tcctgacgct gactctggtg
    39361 gtggaagacc aggtgaaaac ccacagcgaa gccaacctga agtacatgga cttggagaag
    39421 aagtcgaaga cctcctatgc caaatggttt ccgtccgtgg agaaagaggc gaaggagtgg
    39481 ggggaactgc gtcagcggct gggctccggg cagtcctctg tggtgtccta cttcctcaac
    39541 atcacagcat tctgcaagga caataatgaa acggcactgg aagtggagca ggacatcctg
    39601 aacagcttcc gtaaaaacgg ttttgagctg atttcaccgc gctttaacca catgcgcaat
    39661 ttcctgacct gcctgccctt tatggccggg aaagggttgt ttaaacagct gaaagaggcc
    39721 ggtgtggttc agcgcgcaga gagctttaat gttgccaacc tgatgccgtt agtggcggat
    39781 aaccccctga caccggcagg tctgctggcc cccacctacc gtaaccagct ggcgtttatc
    39841 gatattttct tccgggggat gaataacacc aactacaaca tggcggtctg tggcacctcc
    39901 ggggccggta aaaccgggct gatacagcca cttatccgca gcgtgctgga ctccggaggc
    39961 tttgccgtgg tgttcgacat gggggatggc tacaagtccc tgtgtgagaa catgggcggg
    40021 gtgtatctgg acggtgaaac cctgcgtttt aacccgtttg cgaacatcac cgatattgac
    40081 cagtcagcgg agcgtgtccg tgaccagttg tcagtgatgg ccagccccaa cggtaacctg
    40141 gatgaagtgc atgaaggtct gctgttgcag gcggtcagag cctcctggct ggccaaagag
    40201 aacagagcac gtattgatga cgtggtggat ttcctgaaaa acgccagtga cagcgagcag
    40261 tatgccgagt caccgactat ccgcagccgt ctggacgaaa tgattgtgct gcttgaccag
    40321 tacactgcca acggcactta cggccagtat tttaactctg atgagccgtc cctgcgggat
    40381 gacgcaaaaa tggtggtgct ggagctgggc ggactggaag accgtccgtc actgctggtt
    40441 gcggtgatgt tctccctgat tatctacatt gagaacagga tgtaccgcac gccgcgtaac
    40501 ctcaagaaac tgaacgttat tgatgaaggc tggcgtctgc tggacttcaa aaatcacaag
    40561 gtcggtgaat ttattgagaa aggctaccgt acggcccgcc gtcataccgg tgcctatatc
    40621 accatcacac agaacatcgt cgactttgac tctgacaagg cctccagtgc tgcccgcgcg
    40681 gcatggggta actcctccta caaaattatc ctcaaacaga gtgcgaagga gttcgcgaaa
    40741 tacaaccaac tgtatccgga ccagttcctg ccactgcagc gcgacatgat tggtaagttt
    40801 ggtgcggcca aagaccagtg gttcagttcc ttcctgctgc aggtggaaaa ccattcctcc
    40861 tggcaccgtc tgtttgtgga cccgttaagc cgcgccatgt acagctctga cggcccggat
    40921 tttgagtttg tgcagcagaa gcgtaaagag gggctgagta ttcatgaggc ggtgtggcag
    40981 ctggcgtgga agaggtcagg gccggagatg gcttcgctgg aagcctggct ggaagaacat
    41041 gagaaataca ggagtgtggc atgagttcaa cgcagaaacc cgccgacgtc acggcagaac
    41101 gacgcagcca ctggtggtgg acggtgccgg gatgcctggc aatggtgtta ctgaacgcag
    41161 ccgtcagtta cggcattgtc agactgaacg caccggtgac tgtcgccttc aacatgaaac
    41221 agaccgtgga tgcgtttttt gacagcgcca gtcagaaaca actgtcagaa gcgcagtcaa
    41281 aagccctttc agcgcgtttt aacacggcac tggaagccag cctgcaggcg tggcagcaga
    41341 aacaccacgc ggtcattctg gtgtcgcctg ctgtggtaca gggggcaccg gatatcaccc
    41401 gggaaatcca gcaggatatt gcccggcgaa tgagggcgga accatgagat gccgggggct
    41461 gattgccctg ctgatatggg ggcagagtgt ggccgccgcc gatcttggta cctggggcga
    41521 tctgtggccg gtaaaggagc cggacatgct gacggtgatc atgcagcgcc tgacggcact
    41581 ggagcagtcc ggtgagatgg gccggaaaat gaatgccttt aaggagcggg tgatccgcaa
    41641 cagtctgcgg ccccctgccg tgcccggtat cggacgcacg gagaaatacg gcagccggtt
    41701 atttgacccg tccgtcagac tggctgcgga tatccgggat aacgaagggc gggtgtttgc
    41761 ccgtcagggt gaagtgatga atccgctgca gtatgttcct tttaaccaga cgctgtattt
    41821 catcaatggc gatgatccgg cgcaggtggc ctggatgaaa cgccagacgc cgcccacact
    41881 ggagagcaaa attatcctcg tgcagggcag tatcccggag atgcagaagt ctctggacag
    41941 ccgtgtttac tttgaccaga acggtgtgct ctgccagcgc ctcggcattg atcaggtccc
    42001 ggcacgggtg agcgccgttc ccggcgatcg cttcctgaag gtggaattta ttccggcaga
    42061 ggagggcaga aaatgaagcg aagcctgtgg ctgctgatgt tattccttct cgccggtcat
    42121 gtccctgcag cgtctgcgga ttctgcctgt gaggggcgtt ttgtaaaccc gatcacggat
    42181 atctgctgga gctgtatttt cccgctctcg ctgggcagta tcaaagtcag tcagggtaag
    42241 gtccccgaca cggcgaaccc gtcgatgccc attcagattt gtccggcacc gccgccgctg
    42301 ttcaggcgta tcgggctggc cattggttac tgggagccga tggcgctgac ggacgtcacc
    42361 cggtcaccgg gatgcatggt taacctgggc ttcagcctgc cggcttttgg taaaacggca
    42421 cagggaacgg cgaaaaagga tgagaagcag gtgaacgggg cgttctatca cgttcactgg
    42481 tacaaatacc cgctgacgta ctggctgaac atcatcacat cgctgggctg tctggaaggt
    42541 ggtgacctgg atatcgctta tctttctgaa attgacccca cctggacgga cagcagcctg
    42601 accaccattc tcaatccgga agctgtcatc tttgccaatc cgatagcgca gggggcctgt
    42661 gcagcagatg ccattgccag cgcctttaat atgcctctcg atgttctgtt ctggtgtgcc
    42721 ggttcgcagg gcagtatgta cccgttcaat ggctgggtga gtaatgagtc cagtccgttg
    42781 cagtcctccc tgctggtcag tgaacgcatg gcgttcaagc tgcaccgtca gggcatgatt
    42841 atggaaacca tcgggaaaaa taacgccgtc tgtaatgaat atccgtcccc aatcctgccc
    42901 aaagaacgct ggcgttatca gatggtgaat atgtatccgg acagcgggca gtgccatccg
    42961 ttcgggcgca gtgtgatgcg ctgggagacg ggaaaaaatc cgcccaacac aaagaaaaac
    43021 ttcggctacc tgatgtggcg taaacgtaac tgtgtatttc tgtaacttaa acaaaaagag
    43081 gaatatttta aatggaaaag aaattaagca gtaaatacat tcatatgtat atgagtatat
    43141 cccgagtgtt cattcgtttg acaggcaatg tattaaaact gtttcctttt gctatggtga
    43201 tatgccctgt ggtgtggttt tatctttgcc ctgaacatat tatctcagac attgttaacg
    43261 actggcagga tgcagataca ctggaaaaaa tactgcttgt caaactgtat ctgaagacgg
    43321 ttcttattct gacagtggta agcgttgttg tcttgtctct ttcgtcagat aagtttatgt
    43381 ctgttaagcg caggaaatga gtctgtcggt tctcataaat ataactgtgt gtgcatatgt
    43441 aaaatataaa taaaagtgtg ttatgagttt tttatcattt ttacgaaaga taagtaataa
    43501 agaaaaagaa atttttatca gtgaggagcg ttttttctcc ctgctggaaa gggtgagtgc
    43561 ttttgatgtg gtcagagaat gggctgaaaa tggtggagca tattatcctc tgctggagtg
    43621 ccgtcggctt acagatattg aagatgtcag gaaatcagag gatatactga accgactgga
    43681 gtttattgac agaagatttc gggaatttat cagcattatg tctgaatata atcgtaatga
    43741 taaatctaaa aacaggtggg agagggaatt gtctgaatat ccaccggaaa aaatatacca
    43801 ggtccggaga gagggaaacc atctgtcatt aaaaaattac ggtgatgatt ttactctgga
    43861 cagagatact gccgtggcta cggtgcagat tactgtcagc agaaaatgca ttactcagga
    43921 agaaatggat gaattatatg cattgtctga gagactgaaa gaaaagtgcg ccggtaatta
    43981 aattatatca ctgtgtcggg agatgatatg agccggaaaa tatttgtact ttttgccctg
    44041 gctgcaggga tgcatatcac tgttcatgcc tcagacaatg tgaacactcc tgaaaactga
    44101 caattcctga agcagcagga aaatttaagc agacaactac gcgaaaacgt aactgtgtat
    44161 ttttataacc ctgacaagaa aaggataaat gatggaaaaa gaacagaaac aatgtggcct
    44221 gcagctcccc ttgcatcagg atgtatctct ccctgtaccc cgtgacactt tcagtatgga
    44281 gaaaaaggta ataacagcag agcaatacag agactggcaa ttaattgctg ccagtctcag
    44341 gaaaaagata aatggggaaa gaagctaatc aacgattttc gttgtgttta acatggcgtc
    44401 agttatctcc agacacaatc tattgagcgc ctcattatta tcagtattta cgatgttatt
    44461 aataatactg atggctgtat catgaggcat atttctgatg atgctgaata ataatatttt
    44521 taaagccttt atttcatctg ctctctgatt gtccatataa ctgaatgaaa aatcagggcg
    44581 gtcttctgat gtgttattat tcattgtaac tccttgttgg tatgttttta gcctgtcatc
    44641 tcttgcaatc gatatatcag gctgaaatat tttaccacaa cgcgaccggg cgcgtaatcc
    44701 cggaattaat aatccctgaa ataccgagag aatctggttg ttgatatcag atgttggttg
    44761 tagcgattaa ttactgcctg tttgcaggag aattatatga acaaaaaaat atacatttta
    44821 ttcgccctga ctacaggact gtttatctct gcccacgctt cggaaagcgt gaatacgccc
    44881 gaaaaccgcc agttcctgaa gcagcaggaa acactgagtc agcagttacg tgaaacgccg
    44941 gacacacagt tgcaggcatg ggcagagcag caggtcaggg tgaatcccct tcagcattcc
    45001 gataaccagt ttctcgatga actggtccgt aaacagcagg ctgctcagaa ggataaacca
    45061 cagcagggtg ccgtgtattt tgtgtcgttt tccatacccg aagaggggct gaaacggatg
    45121 ctgggtgaaa cccggcacta cggtattccg gccacactgc ggggcatggt gaacaacgac
    45181 ctgaaggcca cggctgaagc cgtgctggca ctggtgaagg acggggctac cgatggagtt
    45241 cagattgacc cgacgctgtt ttcacagtac ggcatccgca gtgtacccgc aatggtggtg
    45301 ttctgcagcc aggggcacga catcatccgg ggaaacctgc gggtcggcca ggcgctggag
    45361 aaagtggccg ccacggggga ctgccggcag gtggctcagg gactgctgga cggcaccggg
    45421 gataagtcgc gatgagttta cctgccgggt attaccggat tgacccggac atccgtgcac
    45481 ttgtggccgc gatgaacgtt cacggattcc gtacatatgc gtcctgtcag gggcatggtt
    45541 ttcctgttac aaagctgccg ccttatattg cctttgcctg tccggtaaaa atggctgccc
    45601 ttctggagca gcgactgcgg caggatgcgg agtccatgat gccccggttg ctgtggggct
    45661 ggtccgtcgg ggcttctttt aacagtgacc ttcagctctg tttccggctt cagcctgaag
    45721 gccctcatca ctggtatcac cggtactgtc gccgctcact gcgtgcagac ttcaggacgc
    45781 tggtccgctt actgaatccg taaatctgtt cccgttaagg aggcattgat ggcgctgaca
    45841 aagaaacagc gggaaaaact gcgtatgaaa ttcggcggtc gttgtgcgta ctgcggctgt
    45901 gaactgccgg agaaaggatg gcatgccgac catgttcagg cggtgctccg caagtcagag
    45961 cagtgcatga aggctgcaga aaagcgcatt ttcaggctga aatccaccgg tgatgttttc
    46021 agaccggagg ccgactgtcc ggaaaacctc gtcccggcct gtgcgccgtg caacctgctg
    46081 aaaacaacat attcgctgga gatgttcaga atgcaggtgt ccctgcaggt tgaacggggg
    46141 cgcagaagca gcgtgaattt caggaccgca gaacggttcg ggctgattca ggtggtgacc
    46201 aaaccagtcg tgttctggtt tgagcaatat cagagggaga gtgaggtatg aaacgtcttc
    46261 tgccattgct gctggggctg gcagtcagtg tggtacaggc tgacagcaac agtgattacc
    46321 gggcgggttc tgattttgcc cgtcagattc aggggcaggg tacaggcagt attcagggct
    46381 ttaagccaca ggagagtatc cccggctata acgcgaatcc ggacgagaca aaatattacg
    46441 gtggtgtgac tgccggcggg gatggcggcc tgaaaaatga tggcaccacg gaatgggcga
    46501 ccggtgaaac cggaaaaacc attacagagt cattcatgaa caagccgaaa gacattctgt
    46561 caccggatgc gccgtttatt cagaccggca gggatgtgga gagccgggct gacagcatcg
    46621 tgggaaatac cgggcagcag tgcagtgcac agcagataaa ccgcagtgaa ttcacaaact
    46681 atacctgtga acgggacacg atggtggagg agttctgcac acggactgcc aggagcacag
    46741 gagactggaa atacacggat gaatatcgtg aggtgacgat accgcactca cagttccgtt
    46801 tcagtatgaa cgggctgaaa ctggtgttca gtgtcacggc tccggtaaca ggcacggtgg
    46861 aaagtgcttc attgtctgta tatgccgctt ttttcttcct gaactcccgt tataccttca
    46921 tgaattcaac gttcaatgtt ggcctggcaa acgggcagtc ggggactttc cccctgtccg
    46981 gggcgacagg tttgccggtg acacaggggc aggtactgac cggcagtggc tgtactgcta
    47041 acggtaactg tctgtccggc gggaacggtg accgacaggt ttatgagaaa ctggccagcg
    47101 gcgcgtccac ctttacactg aaactcagga tgaaggtccg tgataaagaa tgggttccac
    47161 gggtggagtg ggtggagagc tgtccgttta ataaagcgga tggcgtgctg aaaggcactg
    47221 agtgttcaga accggggggt acgaaaacag gagtgatgga aggtaaaacc tggagtatca
    47281 cacaggcgtg ctgggcatac cgggataaat atgtcacaca gtccgcagat aacggcacct
    47341 gtcagaaata tgtcgataat ccggcctgta cgctggcctc acgtcagtgc gcgttttatt
    47401 ccgatgaggg aacctgtctg catgagtatg ccacgtactc ctgcgagtcc agaacgtccg
    47461 gaaaagtgat ggtctgtggt ggtgatgtct tctgccttga cggtgaatgc gataaggcgc
    47521 agagcggaaa aagcagtgat tttggtgagg ctgtatcaca gctggctgcg cttgccgctg
    47581 ctggtaagga tgttgccgcc ctgaatggtg tggatgtcag ggctttcacg ggcgaggcga
    47641 aattctgccg taaggccgca gccggcttca gtaactgctg taaggatggt ggctggggtc
    47701 aggatgtggg gctggcgaag tgtaacagtg aagaaaaggc gctgggaaag gcaaaggaca
    47761 acaaactgac ggtgagtgtt ggcgagttct gttcgaaaaa ggtactcggt gtctgcctgc
    47821 agaaaaagcg cagctactgc cagtttgatt cgaaactggc gcagatagtc cagcagcagg
    47881 ggcgtaacgg tcagttgcgt atcgggtttg gcagcgccag aagtcctgac tgccggggga
    47941 ttacggttga tgagctgcag aaaattcagt tcgacagact ggatttcact aacttctacg
    48001 aagacctgat gaataaccag aaaatcccgg acagtggcgt tctgacgcag aaagtgaaag
    48061 agcagattgc tgaccagctg aagcaggcag gacagtaagg tatggaaaat aaccggggaa
    48121 aataatgaag gtcatattca cttctaatcg tcttgttgat tttcttatcc ggcttttgat
    48181 tacagcgatt gtcatttccc cggtaattat ctggtcgtgg gatacggtaa aagaaaccac
    48241 ggcagacggg atgcttgccg ctgctttcgt tatcctttac tccggtgtgc tcctgtttat
    48301 tctgtatttt tgtttttctg ccctgactga cctgcagaaa gctgatgaaa gaaaaaatga
    48361 tgaaaggaat gaagatgaat aaagcattac tgccactgtt actctgctgc tttctttttc
    48421 cggcatcagg gaaagatgca ggctggcagt ggtataacga gaaaataaat ccgaaggaaa
    48481 aagaaaataa acctgtacct gcagcccccc gtcaggaacc ggatattatg cagaaactgg
    48541 ccgcgctgca gacggcaaca aagcaggcgc tgtacgaagc cattctgtat cccggcgtgg
    48601 ataattttgt gaaatatttc cgtctgcaga attactggac tcagcaggcc gggcttttca
    48661 ccatgagcgc cagaaaggcc atgctggcac atcctgaact ggactataac ctgcagtaca
    48721 gccattacaa cggcacggta cggaaccagc tggcggcaga ccaggcgcag cagcgacagg
    48781 ccattgcgaa actggctgaa cactacggca tcatgttctt ttaccggggg caggacccta
    48841 tcgacgggca gctggcgcag gtcattaatg gtttccggga tacgtatggg ctgagtgtta
    48901 ttcccgtttc cgtggatggc gtgattaatc cgctgttgcc ggattcccgg actgaccagg
    48961 gacaggcaca gcgcctcggc gtgaaatatt tcccggccat gatgctggtt gacccgaagc
    49021 agggaagcgt tcgtccattg tcatacggct ttatttcgca ggatgacctg gcaaaacagt
    49081 tcctgaatgt ctctgaagat tttaaaccga atttttaacc aaaggatata tatgcataca
    49141 ggggatttgt gtttttttac aggtgccagc ctgtttatcg tggggggggt tggttatttc
    49201 tttacaggac caaataataa aggagtttat ctgctgagct cgtttgtctt ctttgtagta
    49261 aatatcataa gtgtatgtgt gctgccagat aatttcttgt ttctttgtgg tgcggttatg
    49321 aatcagtgtg ccgtaccggt tgtttcaatg gcagtcagta ccatgccggt tatcacgaag
    49381 tttgacagtt tcagaaggat gtttgtttgt attatgttgt cagccatatg gtctggagtg
    49441 atgtggtttt ttattatttc attagtgaaa tgactggtgc taatttgtca taaatgccaa
    49501 atccaatagt gaggttgatt accacagacc agaggataaa ccagactgct atccagtgac
    49561 ttctgaataa ctcacaaaaa taaacataga tgattagaaa tagccatatc acaacagcga
    49621 taattgctat aaataacagt attgttacaa ataaatcagc aaaggtttta atggggaggg
    49681 aatgcaataa tgaatatacc agtaaagcaa aagccgcatt acctgataat gactctctta
    49741 tgatgtttct cactatttcc agtttttctt taaaagataa ctttttcata taacacactg
    49801 attaatttca cgaaagttaa gctgattata tacgtgaata aattcatgta aaccacaaca
    49861 ggatattcaa atgataagta aacacagatt ttctttaccc cggctggata ttacaggaat
    49921 gtgggtattt tccctgggtg tctggtttca tatcgtcgcc cgtcttgttt acagcaaacc
    49981 ctggatggct ttttttctgg cggagttgat tgcggccatc cttgtgctgt tcggcgcata
    50041 ccaggtgctg gatgcatgga ttgcccgtgt cagccgggaa gagcgtgagg cactggaagc
    50101 caggcaacag gccatgatgg aagggcagca ggagggggga catgtctctc actaaattac
    50161 tgccggtcat tttgttgata atggccactg gtgtgcgggc ctccacccgg gatgaaattg
    50221 aacgactctg gaatccgcag ggtatggccg cgcagccagc acaaccagca gcaggcacgt
    50281 cagccaggac agaaaagccg gccccccgct ggttccgcct cagcaacggc aggcaggtta
    50341 acctggctga ctggaaggtg gtgctgttta tgcaggggca ttgcccttac tgtcaccagt
    50401 ttgacccagt actgaaacag ctggcacagc agtacggctt ttccgttttt ccctacaccc
    50461 tggatggtca gggggatacc gcctttccgg aggcattacc ggtgccaccg gacgtgatgc
    50521 agaccttctt cccgaatatc ccggtggcca caccgaccac cttcctggtc aacgtcaaca
    50581 cgcttgaggc attaccgctt ttacaggggg caacggatgc cgccggtttt atggcgcgga
    50641 tggataccgt tctgcagatg tacggaggga aaaaaggtgc gaaataagca ggtggtgtta
    50701 ctcattgccg gaatatccgg catcgcgatg ggaataatcg tcagcctgaa tatccccttt
    50761 atccgtcagg ggctttttta tcccgccagc cctgtagaaa ttgtcgtttc gctgtgtctt
    50821 accttttctg tttctgttgt gttttttatg ggggcaattg tgggatggat ttcggtgtct
    50881 gaaatatatt acagacgcat gaccggtctg aatgaatcat cagaaatatc agagggaact
    50941 tatgatgaaa gaaaaaaata aatgatacag aactggaaat caaacaaatt gaaagggaga
    51001 ttgaacgtag ttgtgataat gcaaaaaaat acttctggct ttttgtggtt ttctttgctg
    51061 ccggattgat tgtaaggaac gttatgcatg attttttcag tgccggtatt gattcgtgga
    51121 aagctgaccc ggaattaaat aattttcgtt atatgtggaa catcctgatg tatgtcattc
    51181 cgattatgtt atatgcgctt gcagctggtt ttcttgctgc ggcttccctg tcgccgttgt
    51241 gcgaaataat attcgggagg ggccggatat tcctgatgaa gcgccgtatg cgccgggaga
    51301 acaaattaag ggagggtggc aataatgctt cgcattaaac ctcttctggt tctttgtgcc
    51361 gctttactga cggtcacacc cgtagcgtta gcggatgtga acagcgacat gaatcagttc
    51421 tttaataagc tgggctttgc ttccaatacc acacagccgg gcgtctggca ggggcaggcc
    51481 gccggctatg cttacggcgg ctctctgtat gcccgtactc aggtcaaaaa tgttcagctg
    51541 atttccatga cgctgccgga tatcaatgcc ggatgtggcg gcatcgacgc ctatctcggc
    51601 tcgttcagtt ttattaatgg cgagcagttg cagcgttttg ttaagcagat tatgagcaat
    51661 gctgccggtt acttttttga ccttgccctg cagacaacgg tgccggaaat aaaaaccgca
    51721 aaagacttcc tgcagaaaat ggcaagtgac attaacagta tgaacctcag ttcctgtcag
    51781 gtggcacagg ggcttgtggg gggcctgttc cctcagacgc aggaatctca gcagaaagtt
    51841 tgtcaggaca ttggcagtga aagcaatatc ttcgccgact gggccgcatc ccgtcagggg
    51901 tgcacggtcg gagggcagtc ctcatctgtt cgtgacaaag ccagcgacaa ggacaaggag
    51961 cgggtgctga aaaaccagaa tatcatatgg gaggcgctga aaaaaaacag aatgtttgac
    52021 ggcaacaaag agttgaaaga atttatcatg acgctcaccg gtacgctgat ttttggggaa
    52081 gacagcgaga ttacccccct gccggcgcgt accactgacc gcgatatcct ccgggcgatt
    52141 atggaggggg gaacggcaaa aatttaccac tgcaacgatt cggacaagtg tctgaaggtg
    52201 gtggcggatg caaatgtgac catcgcccgg gataaagccc tgaaatcgca gattacaaaa
    52261 ctgctgacca gtattcagaa taaagcagtg agtgacactc cgcttgacgc ccgggaacag
    52321 gggtttattt ccagcaccac cattcccgtt tttaaatatc tgattgaccc gcagatgctc
    52381 ggtgtgtcca gcagcgtcgt ttaccagctg acggactata tcggctacga catcatgctc
    52441 cagtacattc aggagctgct tcagcaggcc cgggccatga ttgccaccgg gaactatccg
    52501 caggctgtga tggacaatgt tctggagaat ctgaaccagg cacagcaaca gattgctgtc
    52561 ttccagagtc aggtgcaggt ccagcaggat gcgctgctgg ttgtcgaacg tcagatgagc
    52621 tacatgcgcc agcagctttc cgcccgcatg ctcagtcgtt accagaacaa ctatcacttc
    52681 ggagggaatt cactgtgaat gaagtttatg tgattgccgg tggtgagtgg ttgcggaata
    52741 acctgaacgc cattgccgcc tttatgggga cccggacatg ggattccatc gaaaaaattg
    52801 cgctcacatt atctgttctc gcagtggctg tgatgtgggt acagcggcac aacgtgatgg
    52861 atttgctggg ctgggtggtc gtgtttgtgc ttatcagcct gctggttaat gtccgcacat
    52921 cggtgcagat tattgataac agtgacctgg tgcaggtcca tcgtgtggac aatgtgccgg
    52981 tcgggctggc gatgccactt tcactgacga cccgtatcgg gcatgcgatg gtggccggtt
    53041 atgagatggt gttcgctcag cctgacagcg ccacctacag caagacaggc atgctgttcg
    53101 gggcgaatct gattgtgaaa agcaccgatt ttctgtcccg gaacccggaa atcatcaatc
    53161 tgtttcagga ctacgtccag aactgcgtgc tgggtgatat ttacctgaac cataaataca
    53221 cgctggaaga cctgatggct tccgatgacc catacaccat aattttttct cagcccagtc
    53281 cgctacgggg cgtttatgac aacaataata atttcataac ctgtaaggat gcgtcggttt
    53341 cgctgaaaga caggctgaat ctcgatacaa agacgggagg caaaacctgg cattattatg
    53401 tgcagcagat atttggcggc agaccggacc ctgacctgtt attccgggaa ctgctcagtg
    53461 acagctacag ttatttctac ggtgccagcc agtcggccag ccagattatg cgtaagaatg
    53521 tgacaattaa cgccctgaaa gagggtatca ccagtaatgc ggcccgtaac ggtgatactg
    53581 ccagcctggt cagtctggcc accacctcgt cgatggagaa acagcgtctg gcgcatgtag
    53641 ccattggtca tgtgctgatg cgaaacttgc cgatggtaca gaccattctg gtaggcatta
    53701 ccattggtat cttcccgctc ctggtactga cggctgtctt caacaaactg acactgtccg
    53761 tcctgaaagg ctatgtgttt gccctgatgt ggttccagac ctggccgttg ctgtatgcca
    53821 ttctgaacag tgccatgaca ttctatacga agcagaacgg ggctccggtc gtgctctctg
    53881 aactctctca gatacagctg aaatactctg acctggcctc cactgccggg tatctttcag
    53941 ccatgatccc cccgttgtca tggatgatgg taaaaggcct tggtgccgga ttttccagtg
    54001 tgtacagcca tttcgcttcc tctgctatca gcccgactgc cagtgcggca ggtagtgtgg
    54061 ttgacggtaa ttactcctac ggcaacatgc agacggaaaa cgtgaacggc ttcagctgga
    54121 gcaccaacag caccacgtcg tttggtcaga tgatgtacca gaccggcagt ggcgcaactg
    54181 ccacacagac ccgtgacggt aatatggtga tggatgcgag cggggcgatg tcccggttac
    54241 cggtcggcat caatacaacg cgtcagattg cggcggcaca acaggaaatg gcccgggaag
    54301 catcgaacag agcagaaagt gccctgcatg ggttcagcag cagtattgcc agtgcctgga
    54361 acacgctcag tcagtttggt tctaaccggg ggagcagtga ttctgtcacc ggtggcgctg
    54421 acagtacgat gagtgcacag gactccatga tggccagccg tatgcgcagt gcagtggaaa
    54481 gctatgcgaa ggcgcataat atcagtaatg agcaggcgac tcaggaactg gcatcaacag
    54541 gttctaagtt ctcgtatggc ggatatgcag atgctcatgc tgaatggggg attaaaccta
    54601 aaattcttgg tgttggtggt ggagttggtg tcagaggcgg atttaaggga agtcttgatc
    54661 gaacggatga tgattcacat tctgcaagta gtggcagcag ggctacccat gatgctcgac
    54721 atgatatcga tgctaaagcc agtaaggact ttaaagaggc cagcgattac tttaccagtc
    54781 gcaaagtcag tgaatccggc agccatactg acaataatgc cgattcccgt gtggaccagc
    54841 tgtctgcagc cctgaactca gcaaaacaga gttacgacca gtacacgacg aatatgaccc
    54901 gcagccatga atatgctgaa atggcatccc gtactgaaag catgagtggg cagatgagtg
    54961 aagacctgtc gcaacagttt gcacagtatg tgatgaaaca cgctccgcag gatgcagaag
    55021 ctattctgac aaataccagt tcgccggaga ttgcagaacg ccgtcgggca atggcgtggt
    55081 cttttgtgca ggaacaggtg cagcctggtg ttgataatgc ctggcgtgaa tcccgtggtg
    55141 atattggtaa aggaatggag agcgtacctt cgggtggtgg cagccaggat attattgctg
    55201 accatcaggg acatcaggcc attattgagc aaagaacgca ggacagtaat atccgaaatg
    55261 atgtaaaata tcaggttgat aatatggtca cagaataccg aggcaatatc ggggatactc
    55321 agagcagcat acgtggcgaa gaaaatattg taggaagaca gtattctgaa ctacaaaatc
    55381 accataaaac agaagcatta tctcagaata ataaatataa tgaagagcga gaaagtcaaa
    55441 agataatacc tgagccatca gaagagactg tgaaaaaaat gattgatgag aaaaagaatc
    55501 aactgaaggg atcattatga ttactcacta tgatataaaa gcggagacac agagactaaa
    55561 ggatgtactc tctgtagagg gtgttaatat tccgccatta ttacaagtca taaagccagg
    55621 ggggtatgta tttttatgga ttttattatg gccgacattt ctccgtttgc tagcagataa
    55681 agttgatatc cgagatgccg gatttgatat atgttttagt ggcgtaatgg ggtttgtcat
    55741 ttttgttgcc attactaatg gaatgatgtt gtatcttgct atccctaaaa aattcagaga
    55801 tgagtcgaaa gttatatctt ttatgtatga taagaacaaa acttatatct tgtcgtttgt
    55861 gattgttttt tcaatagttt ccttcgccca tacgttctta tttggttttt tatcgataac
    55921 tctgtttgta attttttcct ttatttatac tatagatata aatcgttata atctctctgc
    55981 aatagtctca gtaataggtc tgttcaaaaa agaatctgtt agttaaaagg atattttgga
    56041 tgaaaacaaa aaaactaatg atggttgcac tggtcagttc cactctggcc ctttcagggt
    56101 gtggtgcgat gagcacagca atcaagaagc gtaaccttga ggtgaagact cagatgagtg
    56161 agaccatctg gcttgaaccc gccagcgaac gcacggtatt tctgcagatc aaaaacacgt
    56221 ctgataaaga catgagtggg ctgcagggca aaattgctga tgctgtgaaa gcaaaagggt
    56281 atcaggtggt gacttctccg gataaagcct actactggat tcaggcgaat gtgctgaagg
    56341 ccgataagat ggacctgcgg gagtctcagg gatggctgaa ccgtggttat gaaggcgcag
    56401 cagttggtgc agcgttaggt gccggtatca ccggatataa ctccaattct gccggtgcca
    56461 cactcggtgt gggccttgct gctggtctgg tgggtatggc tgcagatgcg atggtggaag
    56521 atgtgaacta taccatgatc acggatgtgc agattgcaga gcgtactaag tcaacggtga
    56581 caacggataa tgttgccgct cttcgtcagg gcacatcagg tgcgaaaatt cagaccagta
    56641 ctgaaacagg taaccagcat aaatatcaga cccgtgtggt ctcaaatgcg aacaaggtta
    56701 acctgaaatt tgaagaggcg aagcctgttc tcgaagacca gctggccaaa tcaattgcta
    56761 atatattgta atttaggatg agactggata aagctgtacc agtctcatat aaaatcccta
    56821 ttatggaata aagggggcag tcatgttaat cagaaaatgc ataatgttat caatttgttt
    56881 cctcattcag ggatgcataa gtacacaagt atctgaagga actttatata aatataagaa
    56941 taaacatata agagaatttt tgtccgacca ggtaatgtat aac
//
LOCUS       DAZFFG010000018        53480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFFG010000018 DAZFFG010000000
VERSION     DAZFFG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.018"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            257..343
                     /gene="leuL"
                     /locus_tag="ACHJ7J_001427"
     CDS             257..343
                     /gene="leuL"
                     /locus_tag="ACHJ7J_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO4999679.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            436..2007
                     /gene="leuA"
                     /locus_tag="ACHJ7J_001428"
     CDS             436..2007
                     /gene="leuA"
                     /locus_tag="ACHJ7J_001428"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO4999680.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2007..3098
                     /gene="leuB"
                     /locus_tag="ACHJ7J_001429"
     CDS             2007..3098
                     /gene="leuB"
                     /locus_tag="ACHJ7J_001429"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO4999681.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3101..4501
                     /gene="leuC"
                     /locus_tag="ACHJ7J_001430"
     CDS             3101..4501
                     /gene="leuC"
                     /locus_tag="ACHJ7J_001430"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO4999682.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4512..5117
                     /gene="leuD"
                     /locus_tag="ACHJ7J_001431"
     CDS             4512..5117
                     /gene="leuD"
                     /locus_tag="ACHJ7J_001431"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO4999683.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5225..5356)
                     /gene="sgrT"
                     /locus_tag="ACHJ7J_001432"
     CDS             complement(5225..5356)
                     /gene="sgrT"
                     /locus_tag="ACHJ7J_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO4999684.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5445..7103
                     /gene="sgrR"
                     /locus_tag="ACHJ7J_001433"
     CDS             5445..7103
                     /gene="sgrR"
                     /locus_tag="ACHJ7J_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO4999685.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIARIMQQILASHQVTMEIKEISYDQWHKGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPLDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7332..7901)
                     /gene="espY2"
                     /locus_tag="ACHJ7J_001434"
     CDS             complement(7332..7901)
                     /gene="espY2"
                     /locus_tag="ACHJ7J_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFO4999686.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDQLTHWLIK
                     FNELKEHARDPENMAAKASLSPKDTLCVSFFIGDEAIFTLKLQLKENTRTGCIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8143..8242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8273..9256
                     /gene="thiB"
                     /locus_tag="ACHJ7J_001435"
     CDS             8273..9256
                     /gene="thiB"
                     /locus_tag="ACHJ7J_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO4999687.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9232..10842
                     /gene="thiP"
                     /locus_tag="ACHJ7J_001436"
     CDS             9232..10842
                     /gene="thiP"
                     /locus_tag="ACHJ7J_001436"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO4999688.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVIAVALAAFLALWSNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPELLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNSTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10826..11524
                     /gene="thiQ"
                     /locus_tag="ACHJ7J_001437"
     CDS             10826..11524
                     /gene="thiQ"
                     /locus_tag="ACHJ7J_001437"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO4999689.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASALLGITG"
     gene            complement(11638..12402)
                     /gene="yabI"
                     /locus_tag="ACHJ7J_001438"
     CDS             complement(11638..12402)
                     /gene="yabI"
                     /locus_tag="ACHJ7J_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4999690.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12488..13366)
                     /gene="araC"
                     /locus_tag="ACHJ7J_001439"
     CDS             complement(12488..13366)
                     /gene="araC"
                     /locus_tag="ACHJ7J_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO4999691.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13705..15405
                     /gene="araB"
                     /locus_tag="ACHJ7J_001440"
     CDS             13705..15405
                     /gene="araB"
                     /locus_tag="ACHJ7J_001440"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO4999692.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHMP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15416..16918
                     /gene="araA"
                     /locus_tag="ACHJ7J_001441"
     CDS             15416..16918
                     /gene="araA"
                     /locus_tag="ACHJ7J_001441"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO4999693.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17086..17781
                     /gene="araD"
                     /locus_tag="ACHJ7J_001442"
     CDS             17086..17781
                     /gene="araD"
                     /locus_tag="ACHJ7J_001442"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4999694.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDREHGVFVIKPS
                     GVDYSTMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17856..20207
                     /gene="polB"
                     /locus_tag="ACHJ7J_001443"
     CDS             17856..20207
                     /gene="polB"
                     /locus_tag="ACHJ7J_001443"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO4999695.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGNASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20371..23277
                     /gene="rapA"
                     /locus_tag="ACHJ7J_001444"
     CDS             20371..23277
                     /gene="rapA"
                     /locus_tag="ACHJ7J_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO4999696.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDTARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23289..23948
                     /gene="rluA"
                     /locus_tag="ACHJ7J_001445"
     CDS             23289..23948
                     /gene="rluA"
                     /locus_tag="ACHJ7J_001445"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO4999697.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEERKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24066..24881)
                     /gene="djlA"
                     /locus_tag="ACHJ7J_001446"
     CDS             complement(24066..24881)
                     /gene="djlA"
                     /locus_tag="ACHJ7J_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO4999698.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25136..27490
                     /gene="lptD"
                     /locus_tag="ACHJ7J_001447"
     CDS             25136..27490
                     /gene="lptD"
                     /locus_tag="ACHJ7J_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO4999699.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27543..28829
                     /gene="surA"
                     /locus_tag="ACHJ7J_001448"
     CDS             27543..28829
                     /gene="surA"
                     /locus_tag="ACHJ7J_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO4999700.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            28829..29818
                     /gene="pdxA"
                     /locus_tag="ACHJ7J_001449"
     CDS             28829..29818
                     /gene="pdxA"
                     /locus_tag="ACHJ7J_001449"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO4999701.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            29815..30636
                     /gene="rsmA"
                     /locus_tag="ACHJ7J_001450"
     CDS             29815..30636
                     /gene="rsmA"
                     /locus_tag="ACHJ7J_001450"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO4999702.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30639..31016
                     /gene="apaG"
                     /locus_tag="ACHJ7J_001451"
     CDS             30639..31016
                     /gene="apaG"
                     /locus_tag="ACHJ7J_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO4999703.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31023..31871
                     /gene="apaH"
                     /locus_tag="ACHJ7J_001452"
     CDS             31023..31871
                     /gene="apaH"
                     /locus_tag="ACHJ7J_001452"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO4999704.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAAAS"
     gene            complement(31868..32182)
                     /locus_tag="ACHJ7J_001453"
     CDS             complement(31868..32182)
                     /locus_tag="ACHJ7J_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO4999705.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(32185..32418)
                     /locus_tag="ACHJ7J_001454"
     CDS             complement(32185..32418)
                     /locus_tag="ACHJ7J_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO4999706.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(32504..32983)
                     /gene="folA"
                     /locus_tag="ACHJ7J_001455"
     CDS             complement(32504..32983)
                     /gene="folA"
                     /locus_tag="ACHJ7J_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO4999707.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLAKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33175..35037)
                     /gene="kefC"
                     /locus_tag="ACHJ7J_001456"
     CDS             complement(33175..35037)
                     /gene="kefC"
                     /locus_tag="ACHJ7J_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO4999708.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(35030..35560)
                     /gene="kefF"
                     /locus_tag="ACHJ7J_001457"
     CDS             complement(35030..35560)
                     /gene="kefF"
                     /locus_tag="ACHJ7J_001457"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO4999709.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35671..37002)
                     /gene="yaaU"
                     /locus_tag="ACHJ7J_001458"
     CDS             complement(35671..37002)
                     /gene="yaaU"
                     /locus_tag="ACHJ7J_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4999710.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(37061..37348)
                     /gene="fixX"
                     /locus_tag="ACHJ7J_001459"
     CDS             complement(37061..37348)
                     /gene="fixX"
                     /locus_tag="ACHJ7J_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO4999711.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37345..38631)
                     /gene="fixC"
                     /locus_tag="ACHJ7J_001460"
     CDS             complement(37345..38631)
                     /gene="fixC"
                     /locus_tag="ACHJ7J_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4999712.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38682..39623)
                     /gene="fixB"
                     /locus_tag="ACHJ7J_001461"
     CDS             complement(38682..39623)
                     /gene="fixB"
                     /locus_tag="ACHJ7J_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO4999713.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39638..40408)
                     /gene="fixA"
                     /locus_tag="ACHJ7J_001462"
     CDS             complement(39638..40408)
                     /gene="fixA"
                     /locus_tag="ACHJ7J_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO4999714.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPTVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40881..42395
                     /gene="caiT"
                     /locus_tag="ACHJ7J_001463"
     CDS             40881..42395
                     /gene="caiT"
                     /locus_tag="ACHJ7J_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO4999715.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42426..43568
                     /gene="caiA"
                     /locus_tag="ACHJ7J_001464"
     CDS             42426..43568
                     /gene="caiA"
                     /locus_tag="ACHJ7J_001464"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO4999716.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43697..44914
                     /gene="caiB"
                     /locus_tag="ACHJ7J_001465"
     CDS             43697..44914
                     /gene="caiB"
                     /locus_tag="ACHJ7J_001465"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO4999717.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44988..46541
                     /gene="caiC"
                     /locus_tag="ACHJ7J_001466"
     CDS             44988..46541
                     /gene="caiC"
                     /locus_tag="ACHJ7J_001466"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO4999718.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALLADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46650..47435
                     /gene="caiD"
                     /locus_tag="ACHJ7J_001467"
     CDS             46650..47435
                     /gene="caiD"
                     /locus_tag="ACHJ7J_001467"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO4999719.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMMM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47441..48031
                     /gene="caiE"
                     /locus_tag="ACHJ7J_001468"
     CDS             47441..48031
                     /gene="caiE"
                     /locus_tag="ACHJ7J_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO4999720.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGHDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRTVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(48150..48545)
                     /gene="caiF"
                     /locus_tag="ACHJ7J_001469"
     CDS             complement(48150..48545)
                     /gene="caiF"
                     /locus_tag="ACHJ7J_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO4999721.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48806..52027)
                     /gene="carB"
                     /locus_tag="ACHJ7J_001470"
     CDS             complement(48806..52027)
                     /gene="carB"
                     /locus_tag="ACHJ7J_001470"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO4999722.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(52045..53193)
                     /gene="carA"
                     /locus_tag="ACHJ7J_001471"
     CDS             complement(52045..53193)
                     /gene="carA"
                     /locus_tag="ACHJ7J_001471"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO4999723.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        12732 a        14437 c        14092 g        12219 t
ORIGIN      
        1 ttgcaacgcg tgcattttgt ctcctttttc ctcgtggatt agatgcaaaa atttattctg
       61 aaatatgtca accgatgaaa agcgtcggta gttaagcaga aattaatatc gcttgtttta
      121 accacagcag cacaattagc taattttacg gatgcagaac tcacgctggc gggacgtttt
      181 tattgcgtca gggttgacat ccgtttttgt atccagtaac tctaaaagca tatcgcattc
      241 atctggagct gatttaatga ctcacatcgt tcgctttatc ggtctactac tactaaacgc
      301 atcttctttg cgcggtagac gagtgagcgg catccagcat taagccagca cgcagtcaaa
      361 caaaaaaccc gcgccattgc gcgggttttt ttatgcccga agcgaggcgc tctaaaagag
      421 acaaggaccc aaaccatgag ccagcaagtc attattttcg ataccacatt gcgcgacggt
      481 gaacaggcgt tacaggcaag cttgagtgtg aaagaaaaac tgcaaattgc gctggccctt
      541 gagcgtatgg gtgttgacgt gatggaagtc gggttccccg tctcttcgcc gggtgatttt
      601 gaatcagtgc aaaccatcgc tcgccaggtt aaaaacagcc gtgtatgcgc gttagctcgc
      661 tgcgtggaga aagatatcga cgtggcggcc gaatccctga aagtcgccga agccttccgt
      721 attcatacct ttattgccac ttcgccaatg cacatcgcca ccaagctgcg cagcacgctg
      781 gacgaggtga tcgaacgcgc tatctatatg gtgaaacgcg cccgtaatta caccgatgat
      841 gttgaatttt cttgcgaaga tgccgggcgt acacccattg ccgatctggc gcgagtggtc
      901 gaagcggcga ttaatgccgg tgccaccacc atcaacattc cggacaccgt gggctacacc
      961 atgccgtttg agttcgccgg aatcatcagc ggcctgtatg aacgcgtgcc taacatcgac
     1021 aaagccatta tttccgtaca tacccacgac gatttgggcc tggcggtcgg caactcactg
     1081 gcggcggtac atgccggtgc acgccaggtg gaaggtgcaa tgaacgggat cggcgagcgt
     1141 gccggtaact gttccctgga agaagtcatc atggcgatta aagttcgtaa ggatattctc
     1201 aacgtccaca ccgccattaa tcaccaggag atatggcgca ccagccagtt agttagccag
     1261 atttgtaata tgccgatccc ggcaaacaaa gccattgttg gcagcggcgc attcgcacac
     1321 tcctccggta tccaccagga tggcgtgctg aaaaaccgcg aaaactacga aatcatgaca
     1381 ccagaatcta ttggtctgaa ccaaatccag ctgaatctga cctctcgttc ggggcgtgcg
     1441 gcggtgaaac atcgcatgga tgagatgggg tataaagaaa gtgaatataa tttagacaat
     1501 ttgtacgacg ccttcctgaa gctggcggac aaaaaaggtc aggtgtttga ttacgatctg
     1561 gaggcgctgg ccttcatcgg taagcagcaa gaagagccgg agcatttccg tctggattac
     1621 ttcagcgtgc agtctggctc taacgatatc gccaccgccg ccgtcaaact ggcctgtggc
     1681 gaagaagtca aagcagaagc cgccaacggt aacggtccgg tcgatgccgt ctatcaggca
     1741 attaaccgca tcactgacta taacgtcgaa ctggtgaaat acagcctgac cgccaaaggt
     1801 cacggtaaag atgcgctggg tcaggtggat attgtcgcca actacaacgg tcgccgcttc
     1861 cacggcgtcg gcctggccac cgatattgtc gagtcctccg ccaaagccat ggtgcacgta
     1921 ctgaacaata tctggcgtgc cgcagaagtc gaaaaagagt tgcaacgcaa agctcaacac
     1981 aacgaaaaca acaaggaaac cgtgtgatgt cgaagaatta ccatattgcc gttttgcccg
     2041 gagatgggat cgggccggaa gtgatgaccc aggcgctgaa agtgctggat gccgtgcgca
     2101 accgctttgc gatgcgcatc accaccagcc attatgatgt aggcggcgca gccattgata
     2161 accacgggca gccgctgccg cctgcgacgg ttgaaggttg tgagcaagcc gatgccgtgc
     2221 tgtttggctc ggtaggcggt ccgaagtggg aacatttacc accagaccag caaccagaac
     2281 gcggcgcgct gctgcctctg cgtaagcact tcaaattatt cagcaacctg cgcccggcaa
     2341 aactgtatca ggggctggaa gcattctgtc cgctgcgtgc agacattgcc gcaaacggct
     2401 tcgacatcct gtgtgtgcgt gaactgaccg gcggcatcta tttcggtcag ccaaaaggcc
     2461 gcgaaggtag cggacaatat gaaaaagcgt ttgataccga ggtgtatcac cgttttgaga
     2521 ttgaacgtat cgcccgcatc gcgtttgaat ctgcccgcaa gcgtcgccac aaagtcacct
     2581 caatcgacaa agctaacgtg ctgcaatcct ctattttatg gcgtgaaatc gtcaacgaga
     2641 tcgccacgga atacccggat gtcgaactgg cgcatatgta catcgacaac gccaccatgc
     2701 agctgattaa agatccatca cagtttgacg ttctgctgtg ctccaacctg tttggcgaca
     2761 ttctgtctga cgagtgcgct atgatcactg gctcgatggg gatgttgcct tccgccagcc
     2821 tgaacgagca aggttttgga ctgtatgaac cggcgggcgg ctcggcacca gatatcgcag
     2881 gtaaaaacat cgccaacccg attgcgcaaa ttctgtcgct ggcactgctg ctgcgttaca
     2941 gcctggatgc cgatgatgcg gcttccgcca ttgaacgcgc cattaaccgc gcattagaag
     3001 aaggcattcg caccggggat ttagcccgtg gcgctgccgc cgttagtacc gatgaaatgg
     3061 gcgatatcat tgcccgctat gtggcagaag gggtgtaatc atggctaaga cgttatacga
     3121 aaaattgttc gacgctcacg ttgtgtacga agccgaaaac gaaaccccac tgttatatat
     3181 cgaccgccac ctggtgcatg aagtgacctc accgcaggcg ttcgatggtc tgcgcgccca
     3241 cggtcgcccg gtacgtcagc cgggcaaaac cttcgctacc atggatcaca acgtctctac
     3301 ccagaccaaa gacattaatg cctgcggtga aatggcgcgc atccagatgc aggaactgat
     3361 caaaaactgc aaagaatttg gcgtcgaact gtatgacctg aatcacccct atcaagggat
     3421 cgtccacgta atggggccgg aacagggcgt gaccttgccg gggatgacca ttgtctgcgg
     3481 cgactcgcat accgccaccc acggcgcgtt tggcgcactg gcctttggta tcggcacttc
     3541 cgaagttgaa cacgtactgg caacgcaaac cctgaaacag ggccgtgcga agaccatgaa
     3601 aattgaagtc cagggcaaag ccgcgccggg cattaccgca aaagatatcg tgttggcaat
     3661 tatcggtaaa actggcagcg caggcggcac cgggcatgtg gtggagtttt gcggcgaagc
     3721 aatccgtgat ttaagcatgg aaggtcggat gaccctgtgc aatatggcaa tcgaaatggg
     3781 cgcgaaagcc ggtctggttg caccggacga aaccaccttt aactatgtca aaggtcgtct
     3841 gcatgcgccg aaaggcaaag atttcgacga cgccgttgcc tactggaaaa ccctgcaaac
     3901 cgacgaaggc gcaactttcg ataccgttgt cactctgcaa gcagaagaga tttcgccgca
     3961 ggtcacctgg ggcaccaatc caggccaggt gatttccgtg aacgacaata ttcccgatcc
     4021 agcttcgttt gccgatccgg ttgaacgtgc gtcggcagaa aaagcgctgg cctatatggg
     4081 gctgaaaccg ggtattccgc tgaccgaagt agctatcgac aaagtgttta tcggttcctg
     4141 taccaactcg cgcattgaag atttacgcgc ggcggctgaa atcgccaaag gccggaaagt
     4201 cgcgccaggc gtgcaggcac tggtggttcc cggctctggc ccggtaaaag cccaggcgga
     4261 agcggaaggt ctggacaaaa tctttattga agccggtttt gaatggcgct tgcctggctg
     4321 ctcaatgtgt ctggcgatga acaacgaccg tctgaatccg ggcgaacgtt gtgcctccac
     4381 cagcaaccgt aactttgaag gccgtcaggg gcgcggcggg cgcacgcatc tggtcagccc
     4441 ggcaatggct gccgccgcgg ctgtgaccgg acatttcgcc gacattcgca acattaaata
     4501 aggagcacac catggcagag aaatttatca aacacacagg cctggtggtt ccgctggatg
     4561 ccgccaatgt cgataccgat gcaatcatcc cgaaacagtt tttgcagaaa gtgacccgta
     4621 ctggttttgg cgcgcatctg tttaacgact ggcgttttct ggatgaaaaa ggccaacagc
     4681 caaacccgga cttcgtgctg aacttcccgc agtatcaggg ggcttccatt ttgctggcgc
     4741 gggaaaactt cggctgtggc tcctcgcgtg agcacgcgcc ctgggcattg accgactacg
     4801 gttttaaggt ggtgattgcg ccgagttttg ctgacatctt ctacggcaat agctttaaca
     4861 accagctgct gccggtgaaa ttaagcgacg cagaagtgga tgagctgttt gcgctggtga
     4921 aagccaatcc ggggattcat tttgatgtag acctggaagc gcaagaggtg aaagcgggtg
     4981 agaaaaccta tcgctttacc attgatgcct tccgccgcca ctgcatgatg aacggtctgg
     5041 acagcattgg gcttaccttg cagcacgacg acgccattgc ggcttatgaa gcgaagcaac
     5101 ccgcgtttat gcgttagttt gtacaccgtc cggtactcaa tcgccaaaaa gaaaaaagcc
     5161 agcagataat cactgctggc gtggcgatta acaccaatac tcagtcacac atgatgtccg
     5221 caaatcagta gtcagaatga ctcccctccc actgcatcag ttcttcaagc atggttaatc
     5281 gttgtgggac gcttaaccaa cgcaaccagc acaacttcgc tgtcgcggta aaatagtgct
     5341 gataaaactg acgcatgggg cacctccttg cttcatcgtt aggaattatt gacttaatat
     5401 agggaaaata aaattgctgt cttttgcgca ggagttcccc ttttatgcca tctgctcgtc
     5461 tgcaacaaca gttcatccgc ctgtggcaat gctgcgaggg taaatcgcag gacacaacgc
     5521 tcaacgaact ggcagcgtta ttgagctgct cgcgtcgtca tatgcgcacc ctgctcaaca
     5581 ccatgcagga tcgcggctgg ctgacgtggg aagcggaagt cgggcgtggt aaacgctcgc
     5641 gtctgacatt cctctatacc gggctggcgc ttcagcaaca gcgggcggaa gacctgctgg
     5701 agcaggaccg tatcgaccaa ctagtgcagt tagttggcga caaagcgact gtgaggcaaa
     5761 tgctggtttc tcatctgggc cgcagcttcc gccaggggcg gcacatcctg cgcgtgctct
     5821 actatcgtcc gttgcgtaat ctgctacctg gcagcgcatt gcgccgttcc gaaacccata
     5881 tcgcccggca aatcttcagt tcgctaacgc gcataaatga ggaaaatggg gaactggaag
     5941 cagacatcgc ccaccactgg cagcaaatat caccgcttca ctggcgtttc tttttgcgtc
     6001 caggagtcca ttttcatcat ggtcgtgaac tggaaatgga cgacgtgatc gcctctttaa
     6061 aacgaatcaa tacgctgccg ctctattcgc atattgctga cattgtgtcg ccgacgccct
     6121 ggacgctgga tatccacctc acgcagccgg atcgctggtt gccgttactg ctgggacaag
     6181 ttccggcgat gatcctgccg cgcgaatggg aaaccctcag taactttgcc agccatccca
     6241 tcggcaccgg tccgtatgcg gtgattcgca acagcaccaa tcaactgaaa attcaggcat
     6301 tcgatgactt cttcggttac cgggcattaa tcgatgaagt taacgtctgg gttctgccgg
     6361 aaattgccga cgagccagcc ggagggctga tgctgaaagg cccacagggc gaggaaaaag
     6421 agattgaaag ccgactggag gaaggttgtt actatttact gttcgacagc cgaacccatc
     6481 gcggggcgaa tcaacaagtc agagactgga taagctatgt gctttcgccg accaatctgg
     6541 tctatttcgc cgaggaacag taccagcaac tgtggtttcc ggcctatggg ctgctccccc
     6601 gctggcacca cgcccgccca acacattgcg aaaaacctgc cgggctggaa catcttaccc
     6661 tgacgtttta tcaggatcat attgaacatc gggtgattgc caggatcatg cagcagattc
     6721 tggcgagtca tcaggtcacg atggaaatca aagagatcag ctacgatcaa tggcacaaag
     6781 gtgagatcga aagcgacata tggcttaaca gcgccaactt tacgctgccg ctggattttt
     6841 cgctgttcgc gcacttgtgc gaagtacccc tgctgcaaca ctgtattccg ctcgactggc
     6901 aagccgacgc agcccgctgg cgcaatggcg aaatgaacct ggcgaactgg tgccagcaac
     6961 tggtcgccag taaagcaatg gtgccactta tccaccactg gctgatcatt caggggcaac
     7021 gcagtatgcg cggcctgcgc atgaataccc tcggctggtt cgattttaaa tcagcgtggt
     7081 ttgcgccgcc ggatccagag tagcttgcgc cttttggcgg tcatgagcgc attttttata
     7141 ttcagacgga atgataaaat acattacttt tatattttct ctgttttatg catcgtctga
     7201 ttagcgtcgc aaataagatt aaagaagggg cattccagcc cctctttcat attctgagtt
     7261 aatcaataac ggcgcagtct agccttgttc tggccggaaa gatcatcttt ttcaggttaa
     7321 cctcgcgcag gtcagtcata ccaacggcta ttgttcgttt cagcattgct caggtcaacc
     7381 tcgagacagt caataccaca aatgacaacc ccgttaaagt aagcattact cagatcaatg
     7441 cacccggtcc tggtgttctc tttcagctgc aacttcagag taaatatcgc ctcatcgcca
     7501 ataaaaaaac tgacgcataa cgtatctttt ggcgagagtg aggccttcgc ggccatattt
     7561 tccggatctc ttgcgtgttc tttgagttca ttaaatttta tcagccagtg agttaactga
     7621 tcacgcgcct tcccgtcgac agccccggta aagtattttg agtagtcatc actattctgg
     7681 aaataaaatg catccttcag aatttgcctt gccgcagtct gggaatgttt atctgaccgg
     7741 aagagattgc gtaacgtctc ccatagctct tcaaagatat tcatattctc ggtctttttg
     7801 accaagttag ccatcctgat aatctgagaa ttagtaaggc ctggatcttt agtggtatta
     7861 ctagcaggga tactaatctg ttctgggttt cttactttca tgctgatagc tccactctga
     7921 gtaaaaaact cagaataagc gtcctgaata agaatactgt caaaaatcgc tcattcacgc
     7981 aataaacggt tacgttacct attttgactg tagcgaaggg attctttcgg atcaacgctc
     8041 tccagctatg caaatacgct cttgatagct gaacgaatat tggctttttt aatacgcctt
     8101 ctctatggat tgctggttga taacaaaatc actacactaa cgccgttctc aacggggtgc
     8161 cacgcggacg cgtgcgctga gaaaataccc gtcgaacctg atccggataa cgccggcgaa
     8221 gggatttgag gctccttctc aagtcctttg ccactctttt ttgaggtgca aagtgttaaa
     8281 aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac ccgttctgac
     8341 tgtttatacc tacgattcct tcgccgccga ctgggggcca ggtccggtgg ttaaaaaagc
     8401 ctttgaagcc gactgtaatt gcgaactgaa actggtggcg ctggaagatg gcgtttcgct
     8461 tctcaaccgt ctacggatgg aaggcaaaaa cagtaaagcc gatgtggtgc tggggctgga
     8521 taacaacctg ttagacgccg ccagtaaaac cggactgttt gccaaaagcg gtgtggcagc
     8581 ggatgccgtt aacgttcccg gcggctggaa taatgacact ttcgtaccgt ttgattatgg
     8641 ctacttcgcc ttcgtttatg acaagaacaa actgaaaaac ccaccacaaa gcctgaaaga
     8701 actggttgag agcgatcaaa actggcgggt gatttatcag gatccgcgca ccagtacacc
     8761 ggggctgggt ctgttgctat ggatgcaaaa agtctatggc gatgacgccc cacaagcctg
     8821 gcagaaactg gcgaagaaaa cggtcacggt caccaaaggc tggagcgaag cctacggcct
     8881 gtttttaaaa ggtgaaagcg atctggtact gagttacacc acctctccgg cttatcacat
     8941 tctcgaagag aagaaagata actacgccgc cgcgaacttc agcgaaggtc actatctgca
     9001 agtggaagtc gccgctcgca ccgctgccag caagcagccg gagctggcgc aaaaattcct
     9061 ccagtttatg gtttctccgg ctttccagaa tgcgatccca accggcaact ggatgtatcc
     9121 ggtggcaaac gtcacgctgc ctgccggttt tgaaaaattg accaaacccg caaccacgct
     9181 ggagttcacg ccagccgaag tggcggcaca acgtcaggca tggattagcg aatggcaacg
     9241 cgccgtcagc cgttaattcc cggctggtta attccaggtg taagcgccgc cacgctggtg
     9301 atagcggttg cgctggcggc gtttctcgcc ctgtggtcga acgcgccgca gggtgactgg
     9361 tcggcagtct ggcaggacag ctacctgtgg catgtggtgc gcttctcctt ctggcaggcg
     9421 tttctctcgg cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc gctctatcgc
     9481 aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt gatcctcccg
     9541 gtgctggttg ctgttttcgg cattcttagc gtctatggtc gccagggctg gctggcatcg
     9601 ctctgccaat cgctcggtct ggagtggacc ttttcgccct acggcctgca aggtattttg
     9661 ctggcgcacg tattttttaa tctaccgatg gcgagccgct tattactcca ggcactggaa
     9721 aacattcccg gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg ctggcatttt
     9781 ttccgcttcg tcgaatggcc gtggttacgg agacaaatcc cgccggttgc tgcgcttatc
     9841 tttatgctct gtttcgccag cttcgccacc gtactgtcgc tgggcggcgg tccgcaggcg
     9901 accactatcg agctggcaat ctatcaggca ctgagttacg actacgatcc tgcccgcgcg
     9961 gcgatgctgg cgctgatcca gatggtgtgt tgccttgggc tggtgctgtt gagtcagcga
    10021 ctgagtaagg ccattgcgcc cggcaccacg ctgctgcaag gctggcgcga cccggacgat
    10081 cgtctgcata gccgcatttg cgacacggtg ttaattgtgc tggcgctgct gctgttgctg
    10141 ccaccgttac tggcggtgat cgtcgatggg gtaaatcgcc agttgccgga attgctggca
    10201 caaccggtac tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc ggcaggcgta
    10261 ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc gcgaactgcg ggcgcggcag
    10321 aaaatgctgg cgggtcaggc gctggagatg agtggcatgt tgatcctcgc catgccgggg
    10381 attgtgctgg caacgggctt ctttttactg ctcaacagca ccattggcct gccgcaatct
    10441 gctgacggca ttgtgatttt caccaatgcg ttaatggcga tcccctacgc cctgaaagta
    10501 ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca tgttgtgtca gtcgctgggg
    10561 attgaaggct ggtcgcgctt gaaagtggtg gaactgcgcg ctctgaaacg tccgctggca
    10621 caggcgctgg cctttgcttg tgtgctgtcg attggtgatt ttggcgtggt ggcgttgttc
    10681 ggtaacgatg atttccgtac cctgccgttt tatctctacc agcaaattgg ctcctatcgc
    10741 agccaggacg gcgcggtcac cgcgttaatt ctgctactac tctgttttct gctgtttacc
    10801 gtgattgaaa aactaccggg gcgaaatgtt aaaactgact gatatcacct ggctttacca
    10861 ccatttgccg atgcgtttta gcttaacggt ggaacgcggc gagcaggtgg cgatcctcgg
    10921 gccaagcggc gcgggtaaaa gtaccctgct gaatttgatt gccggttttc tgacgccagc
    10981 cagcggttcg ctgactatcg atggcgttga tcacaccacg acgccgccgt cacgccgtcc
    11041 ggtgtcgatg ctgtttcagg agaacaacct gttcagccac ctgacggtcg cgcagaacat
    11101 cgggctgggg ctaaatccgg gattgaaact gaacgcggca cagcaggaga aaatgcacgc
    11161 gattgcccgt cagatgggga ttgataattt aatggcgcgg ttacctggcg agctttccgg
    11221 cggtcagcga cagcgagtgg cgttagcgcg ttgtctggta cgcgaacagc cgattttatt
    11281 gctcgatgaa ccgttctctg cgctcgatcc ggcgttacgt caggagatgt tgacgctggt
    11341 gagcacgagc tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca gcgtggaaga
    11401 tgcggcgcgg atcgccacgc gctcggtggt ggtcgccgac gggcgtatcg cctggcaggg
    11461 taagaccgaa gaattgttga gcggtaaggc gagtgcttcg gcgctgttgg gaattacagg
    11521 ttaatatgca ctgtcggatg cggcgtgaac gccttatccg acctacgggt agtgcgtttt
    11581 gtaggcctga taagacgcgg caagcgtcgc atcaggcaac ccgcacaaga ctatctccta
    11641 aaccccaacc actttacgca aaatatcgat atacaccggc atcaacgggt ggcgaattaa
    11701 caccaccagc gccaccacgc caatggcaga aatcaacggc gtcaaccaca acaaacgacc
    11761 gcgggacaaa taatgactca aacggtcagt cgctttacca ctgcgccata accgccagca
    11821 cagccagcca ccaacccaca aaaataccgc cgtcgccagc agcaaccatt taaactcacc
    11881 gctctgcatt ccggctggaa tatcgatcgc cgcacccgcc agaatccccg gcaggaagta
    11941 aaacggtggc cacagcaggc agccgataat attcggcgtg ataaatttag ccaccggcag
    12001 atccagcatt cccgccacca ttggcaccag cggacgcgtc gggccaacaa aacgaccgac
    12061 cagaatggtg aacatgctgt gttgatgcaa cgcatgttca gttttatcga gtaatgcttt
    12121 gttcttcttc agaaatgacc agcgatgcaa tggcttttta aaacgccagc ccagccagaa
    12181 ggagatccag tcgcccagca gacaaccgac gatccctgcc agccaggcgt gccagaaact
    12241 taactcgccg ctgccaatca gcgctcccag ccccgccatc agcaccgtac cgggtagaat
    12301 caaaccgacc agcgccagcg actccagaaa ggccaccaac accaccgcca tcaatgaata
    12361 cacggtggat tgggtaataa agtgttccag caatgcttgc ataatgtgcc tgtcaaatgg
    12421 acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg
    12481 ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga
    12541 actcgctcgg gctggccccg gtgcattttt taaatactcg cgagaaatag agttgatcgt
    12601 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
    12661 tcgcctggct gatgcgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
    12721 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
    12781 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat
    12841 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
    12901 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
    12961 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaacccgtat
    13021 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
    13081 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg
    13141 gcgggaacag caaaatatca cccggtcggc agacaaattc tcgtccctga tttttcacca
    13201 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
    13261 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcgt
    13321 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc
    13381 cgccattcag agaagaaacc aattgtccat attgcatcag atattgccgt cactgcgtct
    13441 tttactggct cttctcgcta acccaactgg taaccccgct tattaaaagc attctgtaac
    13501 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa
    13561 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca
    13621 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
    13681 cgtttttttg gatggagtga aacgatggcg attgcaattg gcctcgattt tggcagtgat
    13741 tctgtgcgag ctttggcggt ggactgcgcc agcggtgaag agatcgccac cagcgtagag
    13801 tggtatcccc gttggcaaaa agggcagttt tgtgatgccc cgaataacca gttccgtcat
    13861 catccgcgtg actacattga gtcaatggaa gcggcactga aaaccgtgct tgcagagctt
    13921 agcgtcgaac agcgcgcagc tgtggtcggg attggcgttg acagtaccgg ctcgacgccc
    13981 gcaccgattg atgccgacgg taacgtgctg gcgctgcgcc cggagtttgc cgaaaacccg
    14041 aacgcgatgt tcgtattgtg gaaagaccac actgcggttg aagaagcgga agagattacc
    14101 cgtttgtgcc acgcgccggg caacgttgac tactcccgct atattggcgg tatttattcc
    14161 agcgaatggt tctgggcaaa aatcctgcat gtgactcgcc aggacagcgc cgtggcgcaa
    14221 tctgccgcat cgtggattga gctgtgcgac tgggtgccag ctctgctttc cggtaccacc
    14281 cgcccgcagg atattcgtcg cggacgttgc agcgccgggc ataaatctct gtggcacgaa
    14341 agctggggcg gcctgccgcc agccagtttc tttgatgagc tggacccgat cctcaatcgc
    14401 catttgcctt ccccgctgtt cactgacacc tggactgccg atattccggt gggcacctta
    14461 tgcccggaat gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc cggcggcgcg
    14521 tttgactgcc atatgggcgc agttggcgca ggcgcacagc ctaacgcact ggtaaaagtt
    14581 atcggtactt ccacctgcga cattctgatt gccgacaaac agagcgttgg cgagcgggca
    14641 gtgaaaggta tttgcggtca ggttgatggc agcgtggtgc ctggatttat cggtctggaa
    14701 gcaggccaat cggcgtttgg tgatatctac gcctggttcg gtcgcgtact cggctggccg
    14761 ctggaacagc ttgccgccca gcatccggaa ctaaaagagc aaattaacgc cagccagaaa
    14821 caactgcttc cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga tcacatgccg
    14881 gtggtgctcg actggtttaa cggccgccgc acaccgaacg ctaaccaacg cctgaaaggg
    14941 gtgattaccg atcttaacct cgctaccgat gctccgctgc tgttcggcgg tttgattgct
    15001 gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg gatcgccgtc
    15061 aataacgtga tggcgctggg cggcatcgcg cggaaaaacc aggtcattat gcaggcctgc
    15121 tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg accagtgctg tgcgctcggt
    15181 gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag acatcccatc agcccagcaa
    15241 aaaatggcca gtgcggtaga gaaaaccctg caaccgtgca gcgagcaggc acaacgcttt
    15301 gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca ctatcttcca
    15361 acttccgccc cggcacaggc tgcccaggcc gttgcgactc tataaggaca cgataatgac
    15421 gatttttgat aattatgaag tgtggtttgt aattggcagc cagcatctgt atggcccgga
    15481 aaccctgcgt caggtcaccc aacatgccga gcacgttgtt aatgcgctga atacggaagc
    15541 gaaactgccc tgcaaactgg tgttgaaacc gctgggcacc acgccggatg aaatcaccgc
    15601 tatttgccgc gacgcgaatt acgacgatcg ttgcgctggt ctggtggtat ggctgcacac
    15661 cttctccccg gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac cgttgctgca
    15721 attccacacc cagtttaacg cggcgctgcc gtgggacagt atcgatatgg attttatgaa
    15781 cctgaaccag accgcgcacg gcggtcgcga gttcggcttc attggcgcgc gtatgcgtca
    15841 gcaacatgcc gtggttaccg gtcactggca ggataaacaa gcccatgagc gtatcggctc
    15901 ctggatgcgt caggcggtct ctaaacagga tacccgtcat ctgaaagtct gccgttttgg
    15961 cgataacatg cgtgaagtgg cggtcaccga tggcgataaa gttgccgcac aaatcaagtt
    16021 cggtttctcc gtcaatacct gggcggttgg cgatctggtg caggtggtga actccatcag
    16081 cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc tgctacacca tgacgcctgc
    16141 aacacaaatc cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta ttgagctggg
    16201 gatgaagcgt ttcctggaac aaggtggctt ccacgcgttc accaccacct ttgaagattt
    16261 gcacggtctg aaacagcttc ctggtctggc cgtacagcgt ctgatgcagc agggttacgg
    16321 ctttgcgggc gaaggcgact ggaaaactgc cgccctgctt cgcatcatga aggtgatgtc
    16381 aaccggtctg cagggcggca cctcctttat ggaggactac acctatcact tcgagaaagg
    16441 caatgacctg gtgctcggct cccatatgct ggaagtctgc ccgtcgatcg ccgcagaaga
    16501 gaaaccgatc ctcgacgttc agcatctcgg tattggtggt aaggacgatc ctgcccgcct
    16561 gatcttcaat acccaaaccg gcccagcgat tgtcgccagc ttgattgatc tcggcgatcg
    16621 ttaccgtcta ctggttaact gcatcgacac ggtgaaaaca ccgcactccc tgccgaaact
    16681 gccggtggcg aatgcgctgt ggaaagcgca accggatctg ccaactgctt ccgaagcgtg
    16741 gatcctcgct ggtggcgcgc accataccgt cttcagccat gcactgaacc tcaacgatat
    16801 gcgccagttc gccgagatgc acgacattga aatcacggtg attgataacg atacccgcct
    16861 gccagcgttt aaagacgcgc tgcgctggaa cgaagtgtat tacggatttc gtcgctaagt
    16921 agccgcatca ggtatgtaac gcctgatgcg accctggcgc gtcttatcag gcctacgcgt
    16981 tccgactttg taggccggat aagcaaagcg catccggcat tcttttcccc tcgccccttt
    17041 ggggagaggg ccggggtgag ggaaaatgac ctctctggag tcaacatgtt agaagatctc
    17101 aaacgtcagg ttttagaagc caacctggcg ctgccaaaac acaacctggt cacgctcaca
    17161 tggggcaacg tcagcgccgt tgatcgcgag cacggcgtct ttgtgatcaa accttccggc
    17221 gtcgattaca gcaccatgac cgctgacgat atggtcgtgg tcagcatcga aaccggtgaa
    17281 gtggttgaag gtgcgaaaaa gccctcctcc gatacgccaa ctcaccgact actctatcag
    17341 gcattcccgt ccattggcgg cattgtgcac acgcactcgc gccacgccac catctgggcg
    17401 caggcgggtc agtcgattcc agcaaccggc accacccacg ccgactattt ctacggcacc
    17461 attccctgca cccgcaaaat gaccgacgca gaaatcaacg gcgaatatga gtgggaaacc
    17521 ggtaacgtca tcgtagaaac cttcgaaaaa cagggtatcg atgcagcgca aatgcccggc
    17581 gttctggtcc attctcacgg cccatttgca tggggcaaaa atgccgaaga tgcggtgcat
    17641 aacgccatcg tgctggaaga ggtcgcttat atggggatat tctgccgtca gttagcgccg
    17701 cagttaccgg atatgcagca aacgctgctg gataaacact atctgcgtaa gcatggcgcg
    17761 aaggcatatt acgggcagta atgactgtat aaaaccacag ccaatcaaat gaaaccaggc
    17821 tataatcaag cctggttttt tgatggattt tcagcgtggc gcaggcaggt tttatcttaa
    17881 cccgacactg gcgggatacc ccgcaaggaa cagaggtctc cttctggctg gcgacggaca
    17941 acgggccgtt gcaggttttc cttgcgccgc aagagtccgt ggcgtttatt cccgccgatc
    18001 aggttccccg cgctcagcat attttgcggg gtgaacaagg ctttcgcctg acgccgctgg
    18061 cgttaaagga ttttcaccgc cagccggtgt atggccttta ctgtcgcgcc catcgccagt
    18121 tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac cgtctacgag gccgatgtgc
    18181 gcccgccaga acgctatctg atggagcggt ttatcacctc accggtgtgg gtcgagggtg
    18241 atatgcacaa tggcgctatc gttaatgccc gtctgaaacc gcatcccgac tatcgtccgc
    18301 cgctcaagtg ggtttctatt gatattgaaa ccacccgcca cggtgagctg tattgcatcg
    18361 gcctggaggg ctgcgggcag cgcatcgttt atatgctggg gccggagaat ggcaacgctt
    18421 ccgcgctcga tttcgaactg gaatacgtcg ccagtcgccc gcagctactg gaaaaactta
    18481 acgcctggtt tgccaactac gatcctgatg tgatcatcgg ttggaacgtg gtgcaattcg
    18541 atctgcgaat gctgcaaaaa catgccgagc gttatcgtat tccgctgcgg ctggggcgcg
    18601 ataacagcga gctggagtgg cgcgagcacg gctttaaaaa cggcgtcttt tttgcccagg
    18661 ctaaagggcg gctaattatc gacggtatcg aggcgctgaa atccgcgttc tggaatttct
    18721 cttcattctc gctggaaact gtcgctcagg agctattagg cgaaggaaaa tctatcgata
    18781 acccgtggga tcgaatggac gaaattgacc gccgtttcgc cgaagataaa cctgcgctgg
    18841 cgacttataa cctgaaagat tgcgagctgg tgacgcagat tttccataaa actgaaatca
    18901 tgccgttttt gctcgaacga gcaacggtga acggcctgcc ggtagaccgc cacgggggat
    18961 cggtggcggc gtttggtcat ctctattttc cccggatgca tcgcgctggt tatgtcgcgc
    19021 ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg ctacgtgatg gattcgcgac
    19081 ctgggcttta tgattcggtg ctggtgctgg actacaaaag cctgtacccg tcgatcatcc
    19141 gcacctttct gattgatccc gtcgggctgg tggaaggcat ggcgcagcct gatccagagc
    19201 acagcaccga aggctttctc gatgcctggt tctcgcgaga aaaacattgc ctgccggaga
    19261 ttgtgactaa catctggcac gggcgcgatg aagccaaacg tcagggtaat aagccgcttt
    19321 cgcaggcgct gaagatcatt atgaatgcct tttatggcgt gctcggcacc accgcctgcc
    19381 gcttcttcga tccgcggctg gcatcgtcga tcaccatgcg tggtcatcag atcatgcggc
    19441 aaaccaaagc gttgattgaa gcgcagggct acgacgtgat ctacggcgat actgactcaa
    19501 cgtttgtctg gctgaaaggc gcacattcgg aagaagaagc ggcgaaaatc ggtcgtgcac
    19561 tggtgcagca cgttaacgcc tggtgggcgg aaacgctgca aaaacaacag ctgaccagcg
    19621 cactggaact ggagtatgaa acccatttct gccgttttct gatgccaacc attcgcggag
    19681 ccgataccgg cagtaaaaag cgttatgccg ggctgattca ggagggcgac aaacagcgga
    19741 tggtgtttaa agggctggaa accgtgcgta cggactggac gccgctggcc cagcagtttc
    19801 agcaggagct ttacctgcgt atcttccgca acgagccata tcaggagtac gtccgcgaaa
    19861 ccatcgacaa actgatggcg ggtgaactgg atgcgcggct ggtttaccgt aaacgccttc
    19921 gccgtccgct gagcgaatat cagcgtaatg ttccgcctca tgtacgcgcc gctcgccttg
    19981 ccgatgaaga aaaccaaaag cgcggtcgcc ccttgcaata ccagaaccgc ggcaccatta
    20041 agtacgtatg gaccaccaac ggcccggagc cgctggacta ccaacgttca ccactggatt
    20101 acgaacacta tctgacccgc cagctacaac ccgtggcgga gggaatactc ccttttattg
    20161 aggataattt tgctacactt atgaccgggc aacttgggct attttgagca aaaaaagagt
    20221 tcgccagata ccattttgat gcgtgacgaa tgctttgcca tccagtacca tagcgccctt
    20281 tccattcctg gacctgaata acaccactac ctcataagca cggtagcggg tggttattgc
    20341 ctgcaattaa agatatagag ccgaacacat atgcctttta cacttggtca acgctggatc
    20401 agcgatacag aaagcgaatt gggacttgga accgttgtcg cggtggatgc gcgaactgtc
    20461 actttacttt tcccatctac tggtgaaaac cgtctgtacg cacgcagtga ttcccccgtg
    20521 acccgcgtga tgttcaaccc tggtgatacc attaccagcc atgacggctg gcagatgcaa
    20581 gtcgaagaag taaaagaaga aaatggcttg ctgacctata tcggtactcg cctggatact
    20641 gaagagtccg gcgtagccct gcgtgaagtt ttccttgata gcaaactggt gttcagcaaa
    20701 ccgcaggacc gcctgtttgc cgggcagatt gaccgtatgg accgctttgc gctgcgttat
    20761 cgcgcgcgta agtattccag cgaacagttc cgtatgccgt acagcggctt gcgcggtcag
    20821 cgtaccagcc tgatcccgca ccagctcaac atcgctcatg atgttggtcg ccgccacgcg
    20881 ccgcgcgtcc tactggctga cgaagtgggt ttagggaaaa ccattgaagc cgggatgatc
    20941 ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc tgattattgt cccggaaacc
    21001 ttacagcatc agtggctggt agaaatgctg cgccgtttca acctgcgttt tgcgctgttt
    21061 gatgatgagc gttatgccga agctcagcac gatgcttaca acccgttcga taccgaacaa
    21121 cttgtgattt gctcgctgga ttttgcccgt cgtagcaaac agcggctgga acatctctgt
    21181 gaagccgaat gggacctgct ggtggtcgat gaagcccatc acctggtgtg gagcgaagat
    21241 gcgccaagcc gtgaatatca ggccattgaa caactggcag agcacgtgcc gggcgttctg
    21301 ctgctgaccg cgaccccgga acagctgggg atggaaagcc acttcgcccg tctgcgtctg
    21361 ctggacccga accgtttcca cgattttgcg cagttcgttg aagagcagaa aaattatcgt
    21421 ccggttgcgg acgccgttgc catgctgctg gcaggtaata aactgagcaa tgacgaactg
    21481 aacatgctcg gcgagatgat cggcgagcag gatatcgagc cgctgttgca agcagcaaac
    21541 agcgacagcg aagatgccca gagcgcccgt caggaactgg tttcgatgct gatggatcgc
    21601 cacggcacca gccgcgtgct gttccgtaac acccgtaacg gtgtgaaagg cttcccgaaa
    21661 cgcgagttgc acaccattaa gctgccgcta ccgacgcagt atcagacggc tattaaagtc
    21721 tccggcatta tgggcgcacg taaaagtgcg gaagatcgtg ctcgagatat gctctacccg
    21781 gagcgtattt atcaggaatt tgaaggtgat aacgccacct ggtggaactt cgatccgcgc
    21841 gttgagtggc tgatgggcta cctgaccagc catcgctctc agaaagtgct ggtgatctgc
    21901 gctaaagctg ccactgcgct gcaactggag caagtactgc gcgaacgtga aggtatccgc
    21961 gctgctgtgt tccacgaagg catgtcgatt attgagcgtg accgcgctgc cgcctggttt
    22021 gctgaagaag acaccggcgc acaggtactg ctgtgctcgg aaatcggttc tgaaggacgt
    22081 aacttccagt tcgccagcca catggtgatg tttgacctgc cattcaaccc ggatctgctg
    22141 gagcagcgta ttggtcgtct ggatcgtatc ggtcaggcgc acgatattca gatccatgtg
    22201 ccttatctgg aaaaaactgc tcagtcggtg ctggtgcgct ggtatcacga aggtctggat
    22261 gcattcgagc acacctgccc gaccggacgc actatttacg atagcgtcta caacgatctg
    22321 attaactatc tggcttcacc ggatcaaacc gaaggctttg acgatctgat caaaaactgc
    22381 cgcgagcaac atgaagcgct gaaagcacaa ctggaacagg ggcgtgaccg cctgctggaa
    22441 atccactcca acggtggcga aaaagcccag gcactggcag aaagcattga agagcaggat
    22501 gacgatacca acctgatcgc cttcgccatg aacctgttcg atattatcgg tatcaatcag
    22561 gacgatcgcg gcgacaacat gatcgtgctg accccgtccg atcatatgct ggtgccggac
    22621 ttccctggcc tgtcggaaga tggcatcacc atcacctttg atcgtgaagt ggcgctggcg
    22681 cgtgaagatg cacagtttat tacctgggag catccgctga tccgcaacgg tctggatctg
    22741 atcctttctg gcgatactgg tagcagcacg atttcactgt tgaaaaacaa agcgttaccg
    22801 gtaggtacgc tgttggtgga actgatttac gtggtcgaag cccaggcgcc gaagcagttg
    22861 cagctcaacc gcttcctgcc accgacgccg gtacgtatgc tgctggataa aaatggcaac
    22921 aacctggcgg cccaggtgga gtttgaaacc tttaaccgcc agcttaacgc ggtcaaccgt
    22981 cacaccggca gcaaactggt taatgccgtg cagcaggatg ttcacgctat ccttcagctg
    23041 ggtgaagcgc agatcgagaa atcggcccgt gcattgattg ataccgcacg taacgaagcc
    23101 gacgaaaaac tgtctgccga gctgtctcgt ctggaagcgc tgcgcgcagt gaacccgaac
    23161 attcgtgacg acgaactgac cgccattgag agcaaccgtc agcaggtaat ggaaagcctg
    23221 gatcaggcag gctggcgtct ggatgccctg cgtttgatcg ttgtgacgca tcagtaacgg
    23281 agccgaaaat ggggatggaa aactacaatc caccgcagga accctggttg gttatcctgt
    23341 atcaggatga ccacattatg gtggtcaaca agccgagcgg tttgttgtca gtgccgggtc
    23401 gtctggaaga gcgcaaagac agcgtgatga cgcgcattca gcgtgattat ccgcaggcag
    23461 aatcggtgca tcgtctggat atggctacca gcggcgtgat tgtagtggcg ctgaccaaag
    23521 ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga gccgaaaaag cagtatgtgg
    23581 cccgcgtctg ggggcatcca tcccctgcgg aaggtctggt agatctgccg ctgatttgcg
    23641 actggccaaa ccgcccgaaa cagaaagtct gttacgaaac aggtaaacct gcgcagacgg
    23701 aatatgaagt ggtggagtat gcggcggata acacggcaag agtggtgtta aaaccgatta
    23761 ccgggcgttc gcatcaattg cgtgtgcata tgctggcgct gggtcatccg attctcggcg
    23821 atcgttttta tgcatcaccg gaagcgagag cgatggcacc gcgtttgttg ctacatgcag
    23881 agatgttgac gattacccat ccggcgtatg gcaatagtat gacgtttaaa gcgccagcgg
    23941 atttttaata gtcacgctgg cgaagttcgg taggccggag aaggcgttca cgccgcatcc
    24001 ggcaattttg cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg cctacagttt
    24061 gaaacttatt taaatccttt ctgctgcttt atcagctcat acgcctgctg aatttcctgc
    24121 gctttctgct tcgccatctc catcatctca ggcggtaaac ctttcgccac cagcttatcg
    24181 ggatgatgtt cactcatcag cttacggtag gcacgtttga tggtggtcgc atcatccgtc
    24241 ggcttcacgc ccagcacatt acaggcatct tccagcgtcg gaccacgctg cgcttgctgc
    24301 cagttaccac cgccagattg ctgctgataa ccgccgccaa actgtgcacc gccctgcatc
    24361 atgcgcaaaa actggtcaaa ctgggcgcgg gagatcccta attcttcagc aatgacatac
    24421 agcaccgccc gttcattcgg gtgcagtgaa ccatcagcaa acgccgcctg aatctggatc
    24481 tccagaaaca tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa ctggcgcatc
    24541 ttttcgcgca gcgggtaatt gtctgatttt cccacccgga acgcgttttg cgccgcagta
    24601 cgggaagcgc catgaaggtt cattcggtcc atcaactggc tggcgatatg aatatcagcc
    24661 tccgtgacgc gacctttgga tttggttaaa tgccccatca cttcaaaagt ggtggcaaaa
    24721 aacagcgcct gacgctcacg ctggttggcg aaccacgcca ttttacggct acgggcttta
    24781 tcaaacatat ggccaattaa caggcctaac actacgcccc aaaagccgcc gcccatcagt
    24841 aaggccacgg ccacgccaat gatttttccc caatactgca tatattcccc aaatcgacac
    24901 acggatatca gggctatctc ccacaatata aaggtgcttt taccgttttc cggctgcggt
    24961 caattgtggg acatcgccta taatttgcat tatcatacct gtcattcaat gccgtgccta
    25021 acaccactga cgctattcgg acaggattaa cactagcgta gagatgacga gtacgttagt
    25081 ctctgaccgt ttgtcacgcg caacgttacc gatgatggaa caataaaatc aacgtatgaa
    25141 aaaacgtatc cccactctcc tggccaccat gattgccacc gccctttata gtcaacaggg
    25201 actggcagcc gacctcgcct cacagtgcat gttgggcgtg ccaagttatg accgtcctct
    25261 ggtacagggc gataccaatg acttacccgt gactatcaat gctgaccacg cgaaagggga
    25321 ctacccggat gacgccgtgt ttactggcag cgtggatatc atgcagggta acagccgtct
    25381 gcaggccgac gaagtgcagc tccatcaaaa agaggcacca ggacaaccgg agccggtacg
    25441 taccgttgat gcgctcggta atgtccatta cgacgataac caggtgatcc tcaaagggcc
    25501 gaaaggctgg gcgaatctga acaccaaaga taccaacgtc tgggaaggtg attaccagat
    25561 ggtgggtcgc cagggccgcg gtaaagcgga cctgatgaaa caacgtggcg aaaaccgcta
    25621 taccattctg gataacggta gctttacctc ctgtctgccg ggttctgaca cctggagcgt
    25681 ggtaggtagc gaaattattc atgaccgcga agaacaagtt gcggagatct ggaacgcccg
    25741 ctttaaagtt ggcccggtac cgatctttta tagcccctat ttgcagttgc cggtgggtga
    25801 caaacgtcgc tctggtttct tgatcccgaa cgccaagtac accaccacca actactttga
    25861 gttctacctg ccatattact ggaacatcgc gccaaatatg gatgccacca tcacgccgca
    25921 ttatatgcat cgtcgtggca acatcatgtg ggagaacgaa ttccgctacc tctcccaggc
    25981 gggcgctggc ttgatggaac tggactatct gccttcagat aaagtctatg aagatgaaca
    26041 cccgaacgat gacagttcac gtcgttggtt attctactgg aaccactccg gggtcatgga
    26101 tcaggtgtgg cgtttcaacg tcgactacac caaggtcagc gatcctagct acttcaatga
    26161 tttcgataac aagtacggtt ccagtactga cggctacgca acgcaaaaat tcagcgttgg
    26221 ctatgcggtg caaaacttca atgccaccgt ttcaaccaag cagttccagg tcttcagcga
    26281 acagaacacc agtagctact cggcagagcc gcagttagac gttaactact accagaatga
    26341 tgttggtccg tttgatacgc gtatttacgg ccaggcagtg cactttgtta acaccagaga
    26401 cgacatgcct gaagcaaccc gtgttcacct ggagccgacc atcaatttgc cgctctctaa
    26461 taactggggc agcatcaata ccgaagcgaa gttgctggca acccattatc agcaaaccaa
    26521 tcttgactgg tataactcca gaaacacgac caagctggac gaatccgtta accgcgtaat
    26581 gccgcaattc aaagttgacg gcaaaatggt ctttgaacgc gatatggaaa tgctggctcc
    26641 gggttatacc caaacgctgg aaccgcgcgc gcagtatttg tacgtgccat atcgcgatca
    26701 gagcgacatc tataactacg actcatctct gctgcaatct gactactctg gcctgttccg
    26761 ggaccggact tacggcggtc ttgaccgtat tgcctccgct aaccaggtga cgaccggtgt
    26821 cacatctcgc atatatgatg atgctgccgt tgaacgtttt aatatttccg ttggtcaaat
    26881 ctactatttc acggagtctc gcactggcga tgacaacata acatgggaga atgacgacaa
    26941 aacgggctca ttggtgtggg caggcgatac ttactggcgt atctccgagc gttggggatt
    27001 gcgtggcggg attcagtacg atacacgtct ggataacgta gcgaccagta actccagcat
    27061 tgaataccgt cgggatgaag accgtctggt acagctgaat taccgttacg ccagcccgga
    27121 atatattcag gctacgctgc ctaagtacta ttccactgct gagcaatata agaatggtat
    27181 ttcgcaggta ggtgctgtcg ccagctggcc aattgccgat cgttggtcca ttgttggggc
    27241 ctactactac gacaccaatg cgaacaagca agctgactct atgttaggtg tgcaatacag
    27301 ctcctgctgc tatgcaattc gcgtcggtta cgagcggaag ctgaacggtt gggataacga
    27361 taaacaacat gcggtatatg acaacgcaat cggctttaac atcgaacttc gcggcctgag
    27421 ctccaattac ggtctgggta cgcaagagat gctgcgttcg aacattctgc cgtatcaaaa
    27481 cactttgtga tttgttgatt taccacgtaa tccgcagtgc ggttaattga aatggaaaaa
    27541 gtatgaagaa ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat accagtttcg
    27601 ctgcccccca ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc gtgctggaaa
    27661 gcgacgttga tggattaatg cagtcggtaa aactgaacgc tgctcaggca aggcagcagc
    27721 ttcctgatga cgcgacgctg cgccaccaaa tcatggaacg tttgatcatg gatcaaatca
    27781 tcctgcagat ggggcagaaa atgggagtga aaatctccga tgagcagctg gatcaggcga
    27841 ttgctaacat cgcgaaacag aacaacatga cgctggatca gatgcgcagc cgtctggctt
    27901 acgatggtct gaactacaac acctatcgta accagatccg caaagagatg attatctctg
    27961 aagtgcgtaa caacgaggtg cgtcgtcgca tcaccatcct gccgcaggaa gtcgaatccc
    28021 tggcgcagca ggtgggtaac caaaacgacg ccagcactga gctgaacctg agccacatcc
    28081 tgatcccgct gccggaaaac ccgacctctg atcaggtgaa cgaagcggaa agtcaggcgc
    28141 gcgccattgt cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg attgctcatt
    28201 ctgccgacca gcaggcgctg aacggcggcc agatgggctg gggccgtatt caggagttgc
    28261 ccgggatctt cgcccaggca ttaagcaccg cgaagaaagg cgacattgtt ggtccgattc
    28321 gttccggcgt tggcttccat attctgaaag ttaacgacct gcgcggcgaa agcaaaaata
    28381 tctcggtgac cgaagttcat gctcgccata ttctgctgaa accgtcgccg atcatgactg
    28441 acgaacaggc ccgtgtgaaa ctggaacaga ttgctgccga tatcaagagt ggtaaaacga
    28501 cttttgctgc cgcagcgaaa gagttctctc aggatccagg ctctgctaac cagggcggcg
    28561 atctcggctg ggctacacca gatattttcg atccagcctt ccgtgacgcc ctgacccgcc
    28621 tgaacaaagg tcaaatgagt gcaccggttc actcttcatt cggctggcat ttaatcgaac
    28681 tgctggatac ccgtaatgtc gataaaaccg acgctgcgca gaaagatcgt gcataccgca
    28741 tgctgatgaa ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa caacgtgcca
    28801 gcgcctacgt taaaatcctg agcaactaat ggttaaaacc caacgtgttg tgatcactcc
    28861 cggcgagccc gccgggattg gcccggactt agttgtccag cttgcacagc gtgagtggcc
    28921 ggtcgaactg gttgtttgtg ccgatgccac tctccttacc gaccgggcag cgatgctcgg
    28981 tttgccgctc accctccgca cttattcccc caactctcct gcacaaccgc aaactgcggg
    29041 cacattaacg ctacttcctg tcgcgctacg tgaatctgtc actgcggggc agttagcggt
    29101 tgaaaatggg cattatgtgg tggaaacgct ggcgcgagcg tgcgatggct gtctgaacgg
    29161 tgaatttgct gcgctgatca caggccccgt gcataaaggc gtcattaacg acgcaggcat
    29221 tccgtttacc ggtcataccg agtttttcga agagcgttcg caggcgaaaa aagtggtgat
    29281 gatgctggcg accgaagaac ttcgcgtggc gctggcaacg acgcatttac cgctgcgcga
    29341 tatcgcagat gctatcaccc ctgcgctttt gcacgaagtg attgctattt tgcatcacga
    29401 tttgcgcacc aaatttggta ttgccgaacc gcgcattctg gtctgcgggc tgaatccaca
    29461 cgcgggcgaa ggcggtcata tgggtacgga agagatagac accattattc cggtgctcga
    29521 cgagttgcgg gcgcagggga tgaaacttaa cgggccgctg cctgccgata ccctgtttca
    29581 gccgaaatat ctcgataacg ccgacgccgt gctggcgatg taccacgatc agggtcttcc
    29641 cgtgctaaaa taccagggct tcgggcgcgg tgtgaacatt acgctgggcc tgccctttat
    29701 tcgcacatca gtggaccacg gcaccgcgct tgaactggcg ggacgtggcg aagccgatgt
    29761 cggcagtttt attacggcgc ttaatctcgc catcaaaatg attgttaaca cccaatgaat
    29821 aatcgagtcc accagggcca cttagcccgt aaacgcttcg ggcaaaactt tctcaacgat
    29881 cagttcgtga tcgacagcat tgtttctgcc attaacccgc aaaagggcca ggcgatggtc
    29941 gaaatcggcc ccggtctggc tgcattgacc gaaccggtcg gcgaacgtct ggaccagctg
    30001 acggtcatcg aacttgaccg cgatcttgcg gcacgtctgc aaacgcatcc attcttaggc
    30061 ccgaaactga cgatttatca gcaggatgcg atgaccttta actttggcga actggccgag
    30121 aaaatgggtc agccgctgcg tgttttcggc aacctgcctt ataacatctc cacgccgttg
    30181 atgttccatc tgtttagcta tactgatgcc attgccgaca tgcactttat gttgcaaaaa
    30241 gaggtggtga atcgtctggt tgcaggaccg aacagcaagg cgtatggtcg attaagcgtc
    30301 atggcgcaat actattgcaa cgtgatccca gtgctggaag taccgccgtc agcctttaca
    30361 ccaccaccca aagtggattc cgccgtcgtg cgcctggttc ctcatgcaac gatgcctcac
    30421 ccggttaaag atgttcgcgt gttgagccgc atcaccaccg aagcctttaa ccagcgtcgt
    30481 aaaaccattc gtaacagcct cggcaacctg tttagcgtcg aggtgttaac aggaatgggg
    30541 atcgacccgg cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca gatggcgaac
    30601 tatctggcgg agaacgcgcc tttgcaggag agttaacgat gatcaattcg ccccgagtgt
    30661 gtattcaggt tcaaagcgtc tacattgagg cacaatcttc acctgataat gaacgttacg
    30721 tttttgctta taccgtaacc atacgcaatc tggggcgagc gccagtgcag ttgttggggc
    30781 gttactggct gatcaccaat ggcaatggcc gtgaaaccga agtccagggc gaaggagtgg
    30841 ttggcgtcca gccacttatc gcgcctggcg aagagtacca gtacaccagc ggtgcaatca
    30901 ttgaaacccc gctgggcacc atgcagggtc actacgaaat gatcgatgaa aatggcgtcc
    30961 ctttcagcat cgacattccc gtattccgac tcgccgttcc cacactcatt cattaaaaga
    31021 atatggcgac ataccttatt ggcgacgttc atggttgtta cgatgaactg atcgcattgc
    31081 tgcataaagt agaatttacc cctgggaaag ataccctctg gctgacgggc gatctggtcg
    31141 cgcgcggccc gggttcgctg gatgttctgc gctatgtgaa atccttaggc gacagcgtac
    31201 gtctggtgct gggcaatcac gatctgcatc tgctggcggt atttgccggg atcagccgca
    31261 ataaaccgaa agatcgcctg acaccgctgc tggaagcgcc ggatgccgac gagctgctta
    31321 actggctgcg tcgccagcct ctgctgcaaa tcgacgaaga gaaaaagctg gtgatggccc
    31381 acgcagggat cacgccgcag tgggatctgc agaccgccaa agagtgcgcg cgcgatgtag
    31441 aagcggtgct gtcgagtgac tcctatccct tctttctcga cgctatgtac ggcgatatgc
    31501 caaataactg gtcaccggaa ttgcgggggc tgggaagact gcggtttatc accaacgcct
    31561 ttacccgtat gcgtttttgc ttcccgaacg gtcaactgga tatgtacagc aaagaatcgc
    31621 cggaagaggc ccctgcccca ctgaaaccgt ggtttgcgat tcctggacct gtcgctgaag
    31681 aatacagcat cgcctttggt cactgggcat cgctggaagg caaaggtacg ccggaaggta
    31741 tttacgcgct ggatactggc tgctgctggg gtgggtcatt aacctgcctg cgctgggaag
    31801 ataaacagta ttttgtccag ccgtcgaaca gacataagga tatgggcgaa ggagaggcgg
    31861 ccgcgtctta aatcccgtcg agcataaaat ctaatgctcc tttaatcaac gttcggtgcg
    31921 cgctggcatc gcaaaatttc gttcccagcg cgttaactgg aacagttgca gtctcatgcg
    31981 tcatgagcac atattctttg ccgtctacca gtaaaaggat tgggttaagc cgttctggtg
    32041 ggcgaatacg gctaaatcgt tcaataggtg ttaatggaat aacaatacgg cgattaatca
    32101 caccaataat gtcgctggta acatcgataa caaagggaaa cgcggcgttc ctgctgcgac
    32161 tgcggtatac cgtaaattgc atcgctagaa gctccggtac tcatcagaga aacatccggt
    32221 ttcatcagcc aattgattta acgcagcgat agcttctgcg ttctcttcac gccaacgtgt
    32281 tgctgcatgt tttttaagtt cagcattgag cgcaaccgta agggtggcgc tcatatttaa
    32341 gcctgcatca cgagcgcgat tgactaactc acggtcgacg gtgacggtca cactttgtgt
    32401 ggtacgtttt gcagtcatga tatcgtgctc ctgtacaatt tatacgtcta gtatacacgg
    32461 atatctgcac gtatcagtga tgccggaatt ctaatataca aaattaccgc cgctccagaa
    32521 tctcaaagca atagctatga gagttctgcg catcagcgtc gtggaattcg ctgaataccg
    32581 attcccagtc atccggctcg taatccggga aatgggtgtc gccttccact tctgcgtcga
    32641 tatgcgtcag atacagtttt tgcgcttttg ccaggaactg ctcataaacg cgaccgccgc
    32701 caatcaccat gatttctggt acgtcaccac acgccgcgat ggcttcatcc accgacttca
    32761 cccacgttac gcgatcgtcc gtacccggtt gactgctgag gataatattt ttgcgtcctg
    32821 gcaacggacg accgatggat tcccaggtat ggcggcccat aatcacgggt ttatttaagg
    32881 tgttgcgttt aaaccaggcg agatcggcag gcaggttcca cggcatggcg ttttccatgc
    32941 cgataacgcg atctaccgct aacgccgcaa tcagactgat cattgagatt tcccgataaa
    33001 aaaaattgtc gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt
    33061 tgaggaaaat tttatattct gctggcgagt ccacgctctc cccctggact cgccgcatta
    33121 caatgaaaca aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattagga
    33181 tgagggtttc gtttccggtt catccgccat gttgccggta tgtttacctt cttctgttcc
    33241 ctgccagcca tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga tgatctcact
    33301 taacatcgcg ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat
    33361 tgccatctct tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc
    33421 ttcatacggc cccagaccta aactttccag cgccagacgc ccggttttca gcgcaccttc
    33481 aaaggtttca cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtggtcgac
    33541 atcgcgggcg cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctccgtcag
    33601 ttgcaggttg gtttgcggat cgtcaatagc gttaatcagc acttccgctt tcgccgctcc
    33661 ggcagattcc agtaaatcca tccgcgtggc atcgccgtaa aacactttca taccaaattt
    33721 acgcagcgtt tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga
    33781 gagcagtaaa cgtccggtaa tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg
    33841 ctgttcttca tcgatctcat cggcttcacg cgcttcctca gtagaagatt gctcaaggcg
    33901 attgaggatc accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag
    33961 cgatttcgcc cactccggct ccagcacatt cgccatctgc gccgcgccaa ataccacaaa
    34021 ggcaaactca ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt tatttggcac
    34081 ctgcaacggt cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag
    34141 caaaatgaca atgcgcaatg ggttttcaat cagcgtgcca aagtctatcg acatgccaac
    34201 accgatgaaa aacagcccca acagcaaacc tttaaacggt tcgatatcgc tttccagcgc
    34261 atgacggtat tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga
    34321 caagccgacc tcttccagca gcaaaccaaa accaaacaca aggaataacg ccacggcact
    34381 aaacacttcc cgcaagccag agcgggcaac aaaacgcagc gccggacgcg taacatagcg
    34441 ccccagcaat accaccagta ccagcgcacc cgccactttt aacgccgaga gagcaaatgc
    34501 gcccatcgtc gtcgaggcac tgctcgccgc cagtagcgga atcatcgcca ccaacgggat
    34561 cgccgcgata tcctggaaca gcagcaccgc aaaggcactg cgacccattt gcgtcaccat
    34621 cagattgcgt tcattcatcg cctgcatggc aatcgccgta gaggagagcg ccagcgtcat
    34681 gccgatcaat tccgcgacct gccagcgcag cccaagtaac atacagaaca gccccagcag
    34741 gccaccgcaa atcaccatct gcaatgcgcc gccgccgaac actgccgcac gcagcttcca
    34801 cagcctttgt ggatcgagtt cgaggccgat aataaacagc atcagtacca cgccaatctc
    34861 ggcaaagtgc agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat
    34921 gcagccggcg atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac
    34981 aatcagcgcc gccgatccga ggtagatcag cgcctgaacc agcgtatgac tatccatgat
    35041 gtgcctcctg ccattccagc agacgttgct tatagtggcg cgcctgccct tcgagggttt
    35101 cgtcgtcaca aataaaggta cagtgcatgg caaacggtgg cagccagttc agcccgcagt
    35161 agattgccgt cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt
    35221 caaaatggct ttccccgccg ccggtcgtca ccgcccatag caaatgtttg ccatgcagcg
    35281 ccgtgccgcc gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc caaagtttaa
    35341 gaagcggagg aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag
    35401 agagcgcctc ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag
    35461 agcgaatttc gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg
    35521 aatgatgcgg atacggatgc gcataaatga tgaggatcat agttattagc ccgcgtgttt
    35581 acgcctttgt tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat
    35641 tgattcagaa aagagagaaa tctgaacaaa ttatcccatt ctctgcccgc ggatcgtcat
    35701 attgctggtc tgcgccagtg acattcctcg agtctccggg gcaaacgcta cggaaatcaa
    35761 caagccaaac agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt
    35821 gataaatatc ggcagtgccc aggtcgaaac aatggtgcca atacgactta aggacataat
    35881 cacgcccacg gcagaggcgc ggatatccgt cgggaagagt tcattaggat agagccactg
    35941 caaattaccc ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca
    36001 gatccccata tccgggatta gccccaaaac cgccagcgcc agcgtcatca tggcaaagct
    36061 gccaatcaac aatggacgcc gtccggcagt gtttagccac agcatcggcg gaatacagcc
    36121 gagcataaag aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc caacccccaa
    36181 tcccaacaaa ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg
    36241 gcaggtccag atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc
    36301 gcgaaaacgg gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt
    36361 tatcatcatc tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc
    36421 aggcagttcg aatcgaccaa tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat
    36481 ccagcgccag ccgccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc
    36541 gccgacatac cacatggcgg cgataaagct gatggaaaat gcccgctggc gggtactgga
    36601 gaactcggtg atcattgagg tggcgatggg ataatctgca ccgatgacaa tgccgataag
    36661 tacccgcatc accaacagtt cgacggggga tgaaacaaac atcgtcgcca cagatatcac
    36721 accgatggcg atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggaaatata
    36781 gccaaacagc gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat
    36841 ccagtcagcg tccagtttta gcgccggagt cagttgctcc agcgccacgc caatcattac
    36901 cagtacataa ccatccaaaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat
    36961 tgaggagaat ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt
    37021 tgcaatgaga gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca
    37081 caccaaaggt gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc
    37141 gacaggtgcc gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct
    37201 tcttgtacag acctgcgggg cacgctttca ccagcagctc cagggcctgt ttatcggcat
    37261 cggccttcac aacaatgtgt ggatgctctt catcgacatt gaatttattg acgcccagtt
    37321 tgacgtccac attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat
    37381 cagattgatg aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg
    37441 cgcactgccg tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggtagcc
    37501 gctaaacatg cgtgggttat caaggaacgc cggtagtttc tggtacatgc gcatatcgcg
    37561 cagcgggcca ctctccagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc
    37621 gcttttcatc gctgaaagca cggtttttgc tgcggcttcc ccggcggcaa tcgccagatc
    37681 cataccgcga atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa
    37741 tacgccgtca ccaaccaact ccggcagcat gttgatgcct gcttccggca ctacgtgagc
    37801 ggaatattcc accagcttgc cacccgcgat cagcggtgca acggccggat gttgtttgaa
    37861 atcttccagc atttgcggca ctgatttttt cgcgtcatgc agatgatgca gaccacaaac
    37921 cagccccagc gacagggtgt tttcattggt ataaagaaaa ccgccgccca tcaggccatc
    37981 agtaggtgat cccgcaaaca ggcaagccgc gccctgatta ccctgcaact gaaaacggtc
    38041 ttcgattacc gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg
    38101 tttgacgcgt tttgccatcc ccaatttttc ggcaaggatg gagttcaccc catcagcaag
    38161 gatcaccgtt ttcgcttcaa tcacatcgcc atcggcttct acaccgacga ctttgccatc
    38221 gcgctgtacg aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc
    38281 ctgctccatc aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatgg
    38341 cgaggtttcg tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc
    38401 gagtttttca tgggtgatca ggcgttctac gggggcggat tcggcgaaac ctggaataat
    38461 gtgttccaga ctgtgggcat agagacgccc gccggtgacg ttcttggcac ctgcggaatt
    38521 gccacgctcg ataactaaca cttgcgcacc ttcgcgggcg agcaccagtg cggcaaccga
    38581 accggcaagc cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc
    38641 ctgtcagcgg cagggccgta atgcagccct gccagagtgg atcaacgcgc taaagctgcg
    38701 gtcagcgccg gaaggatctt cacggcgtcg ccaacaatgc cgtaatccgc gtactggaag
    38761 atcggcgcat ttttatcttt gttgatggcg aaaatggttt gcgatgcatt agcaccaacc
    38821 atgtgctgga tctgcccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg
    38881 ttggagatac cgacatagcg ttcgtgctcc atccattttt cgttttccgc caccggacga
    38941 gaacaggcca actccgcacc tatcgcctta caaagctgtt ctgccagcgc aatgttctct
    39001 ttgctgccaa tgccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg
    39061 ttgctctggc gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc
    39121 acggtgtgcg tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtgccactg
    39181 ctgatggtca gtaccgcata cggcgtggca atgcgttctt cgccaatcgc caggccgccg
    39241 taaaccatgt gtttcactgt tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta
    39301 gacaccgccg ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg
    39361 tttggcaaca gcaccaggcc gtctgcgccg tgctggcgaa tagtgtcagc catgacactg
    39421 gcgtaatctt cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg
    39481 ccgagctgga ttgcctgtgc gccgtcggca tcattgagga caaaggtgtt gatttgatta
    39541 gctaaagcct gcgcaccgtt catcagttcc ggcagacgag aaggggtatc gctgaatacc
    39601 cagacttgag aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt
    39661 cagcaaatgc ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc
    39721 gctgtttcgg cgcggcaacc tgttgttctg accaggctgc ctctgcgtta aaaccgatat
    39781 ccgccgccga ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag
    39841 gaatttgtgg ggagttgata tcagtggaaa cagcaacaac cgtaggcagc ggaatgctta
    39901 aggtttcggt ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga
    39961 taattttgct gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa
    40021 cctgctgggc ataaaggtcg gaagaaccat cgccacagag gatcagatca aagcctgctt
    40081 tctgggcggc tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt
    40141 catcaatcac cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg
    40201 cgttggtcag ggctttaccg cccacactta aggctgtcac ctgcgcctct gctgcctgtt
    40261 gctttagctg gcaagccgct tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg
    40321 ctttgctgaa gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca
    40381 cgcacttata gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt
    40441 tcatatcggt ggtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat
    40501 caccaatatt gaaaatgtca cacgcagata aatccaactt tcaatattgt taagttcctc
    40561 accaatattg aaaacatggc gcagcaaaaa gaaattttca atattgtttt atagatcacc
    40621 aatattgaaa gtcagatgct atttttttac ccacgaaaga acataacaaa atgattaata
    40681 accaaaatta acggaaaaac gtcatctatt cacccgacac aaaaatgtga taacaatcac
    40741 agaatgcagc ttattgaata cccattatga gttagccatt aacgcgtcca cgaggttaat
    40801 aataattata ttaaatgtta acaaaaataa aacaaacggg aacgcaaaat aaatattcgt
    40861 tttcacagtg gaattaactc atgaagaatg aaaagagaaa aacgggaata gaaccgaagg
    40921 ttttctttcc gccattaata atcgtcggca tactttgttg gcttacagtc agagatctgg
    40981 atgcggcgaa tgtcgttatt aatgctgtat tcagttacgt caccaatgta tggggatggg
    41041 catttgaatg gtatatggtg gtgatgcttt tcggttggtt ctggctggtg tttggcccgt
    41101 atgccaaaaa gcgtttaggt aacgaaccgc cagaatttag caccgccagt tggatcttta
    41161 tgatgttcgc ctcctgtacg tctgctgccg tactgttctg gggatcgatt gagatctact
    41221 actacatctc caccccgccg tttggcttag aaccgaactc gacaggagcg aaagagttgg
    41281 ggctggctta cagcttgttc cactggggac ctctgccgtg ggccacttac agcttccttt
    41341 cagtcgcctt cgcttacttc ttctttgtcc gcaaaatgga agtgattcgc cccagctcga
    41401 cactggtgcc gctggtaggt gaaaaacacg ccaaagggtt gttcggtacc atcgtcgaca
    41461 acttctatct cgtcgccttg atcttcgcga tgggtaccag tctgggcctt gccacgccgc
    41521 tggtgaccga gtgtatgcaa tggttgtttg gcattccaca taccctgcaa ctggacgcta
    41581 tcatcattac ctgctggatt atcctcaacg ccatttgcgt cgcctgcggt ctgcaaaaag
    41641 gggtacgtat cgccagtgac gtgcgtagtt acctgagctt cctgatgctg ggttgggtgt
    41701 tcattgtcag cggtgccagc ttcatcatga actacttcac cgattcggtg gggatgttgc
    41761 tgatgtatct gccgcgaatg ttgttctata ccgatcccat cgctaaaggc ggcttcccgc
    41821 agggctggac cgtgttctac tgggcatggt gggtgattta cgccatccag atgagtatct
    41881 tcctcgcccg catctcccgt ggtcgtactg tgcgtgaact gtgcttcggc atggtgatgg
    41941 ggctgacagc atccacctgg atcctgtgga ccgtgctcgg tagtaacact ctgctgttga
    42001 tggataaaaa catcatcaac attccaaatc tgatcgaaca gtacggtgtg gcgcgcgcca
    42061 tcatcgaaac ctgggccgct ctgccgctca gcaccgccac catgtggggc ttcttcatcc
    42121 tctgctttat tgccaccgtt acgctggtta acgcctgctc ttataccctg gcgatgtcca
    42181 cttgccgcga agtacgcgat ggtgaagaac caccgctgct ggtgcgtatc ggttggtcaa
    42241 ttctggttgg cattatcggt attgttttgc tggcgctcgg cggcctgaaa ccgattcaaa
    42301 ccgccattat cgccggagga tgcccgctgt tcttcgtcaa cattatggtg acgctctcct
    42361 ttattaaaga cgcgaaacag aactggaaag attaattaac ccccaaaata tcaagaggtt
    42421 gaaagatgga ttttaattta aatgatgagc aggaactgtt tgtcgccggt atccgcgaac
    42481 tgatggccag cgaaaactgg gaggcctatt ttgccgagtg cgaccgtgac agcgtctacc
    42541 cggaacgttt tgtcaaagca ctggcggata tgggtatcga cagtctgctg atccctgaag
    42601 agcacggtgg tctggacgcg gggtttgtta ctctcgccgc cgtgtggatg gagctgggac
    42661 gtctgggggc accaacctat gtactgtacc agctgccggg cgggttcaat accttcctgc
    42721 gcgaaggcac acaagagcag atcgacaaga ttatggcttt ccgcggcacc ggtaagcaga
    42781 tgtggaactc agcgattacc gaaccgggcg cgggctccga cgtgggtagc ctgaaaacga
    42841 cttatacccg tagaaatggt aagatttatc ttaatggtag taagtgtttt attaccagta
    42901 gcgcctacac cccgtacatc gtggtgatgg cgcgcgacgg agcttctccg gacaaacctg
    42961 tctacaccga atggtttgtt gatatgagca aaccgggcat caaagtgacc aaacttgaaa
    43021 agctcggtct gcgtatggat agctgctgtg aaatcacctt tgacgacgtg gaactggacg
    43081 agaaagacat gttcggtcgg gaaggtaacg gctttaaccg cgtcaaagaa gagttcgacc
    43141 atgaacgttt cctggtagcc ctgaccaact acggtacggc gatgtgcgcc tttgaagatg
    43201 cggcgcgcta cgccaaccag cgcgtgcagt ttggcgaggc tattggtcgt ttccagttga
    43261 ttcaggaaaa attcgcccac atggcgatca aattaaactc catgaaaaac atgctgtatg
    43321 aagcagcgtg gaaagcagac aacggcacca tcacctctgg cgatgcagcg atgtgcaaat
    43381 acttctgcgc caatgcggca tttgaagtgg tggatagcgc aatgcaggtg ctgggcggtg
    43441 tcggaattgc tggcaaccac cgcatcagcc gcttctggcg tgatctgcgt gtagaccgcg
    43501 tctccggggg atctgacgaa atgcagatcc tgacgctggg tcgtgcggtg ctgaagcaat
    43561 accgctaagt ccgtgcgctg cctgatgcga cgctgacgcg tcttatcagg cctacgggag
    43621 cacgaatgta ggccggataa ggcgtttacg ccgcatccgg cagtcatgcg ctacataaat
    43681 ttttcaggag ctaataatgg atcatctacc catgccgaaa ttcggaccgc tggccggatt
    43741 gcgcgttgtc ttctccggta tcgaaatcgc cggaccgttt gccggacaaa tgttcgcaga
    43801 atggggcgcg gaagttattt ggatcgagaa cgtcgcctgg gccgacacca ttcgcgttca
    43861 accgaactac ccgcagctct cccgccgcaa tttgcacgcg ctgtcgttaa atattttcaa
    43921 agatgaaggc cgcgaagcgt ttctgaaatt aatggaaacc accgatatct tcatcgaagc
    43981 cagtaaaggt ccggcctttg cccgtcgtgg cattaccgat gaagtactgt ggcagcacaa
    44041 cccgaaactg gttatcgctc acctgtccgg ttttggtcag tacggcaccg aggagtacac
    44101 caatcttccg gcctataaca ccatcgccca ggccttcagt ggttacctga ttcagaacgg
    44161 tgatgttgac cagccaatgc ctgccttccc gtataccgcc gattactttt ctggcctgac
    44221 cgccaccacg gcggcgctgg cagcactgca taaagcgcgt gaaaccggta aaggcgaaag
    44281 tatcgacatc gccatgtatg aagtgatgct gcgtatgggc cagtacttca tgatggatta
    44341 cttcaacggc ggcgaaatgt gcccgcgcat gagcaaaggt aaagatccct actacgccgg
    44401 ttgcggtctg tataaatgcg ccgacggcta catcgtgatg gaactggtgg gcattaccca
    44461 aattgaagag tgctttaaag atattggcct cgcacatctg ctcggtacgc cggaaatccc
    44521 ggaaggcact cagcttatcc accgtatcga atgcccttac ggcccactgg tagaagaaaa
    44581 actcgatgcc tggctggcgg cacataccat cgcagaagtt aaagaacgct ttgccgaact
    44641 gaatatcgcc tgtgccaaag tactgaccgt accggaactg gaaagcaatc cacagtatgt
    44701 tgcccgtgaa tcaatcactc agtggcaaac gatggatggt cgcacctgca aagggccgaa
    44761 catcatgccg aaattcaaaa ataaccccgg acaaatctgg cgcggaatgc cctcacatgg
    44821 catggacacg gctgccattt tgaaaaatat cggctacagc gaaaacgaca ttcaggagtt
    44881 ggtcagcaaa ggtctggcca aagttgagga ctaaacgcgt ggcgtcagtc ggcttgcctg
    44941 actaacgccc gcccaataac aaacgaacaa aaatggatag aggtgcaatg gatatcattg
    45001 gcggacaaca tctacgtcaa atgtgggacg atctggcgga cgtttacggt cataaaacgg
    45061 cgctgatttg tgaatccagc ggcggagtcg ttaaccggta tagttatctt gagttaaatc
    45121 aggagattaa ccgcacggca aacctgtttt atacgctggg gattcgcaaa ggcgacaagg
    45181 ttgcactaca tctcgacaac tgcccggaat ttatcttttg ctggttcggg ctggcaaaaa
    45241 ttggcgcgat tatggtgccg attaacgccc gcctgttacg cgaggaaagc gcgtggatcc
    45301 tgcaaaatag ccaggcgtgc ctgctggtga ccagtgcgca attttatcct atgtatcaac
    45361 agattcagca ggaagatgcc actcaattgc ggcacatttg cctgacagat gtggcacttc
    45421 tcgctgatga tggcgtgagt tcgtttactc aactgaaaaa tcaacaacct gccaccttgt
    45481 gctatgcacc gccgctatcg actgacgata cggcggaaat tctcttcacc tccggcacca
    45541 cctcccgacc gaaaggtgtg gtgattaccc attacaacct gcgcttcgct ggatattact
    45601 ccgcctggca gtgtgcactg cgtgacgatg acgtctacct gacggtaatg cctgcgtttc
    45661 atatcgattg ccagtgtact gcagcgatgg cggcgttttc tgccggtgcc acctttgtgc
    45721 tggtcgagaa atacagcgcc cgcgccttct ggggacaggt gcagaagtac cgcgccacca
    45781 ttaccgaatg tattccgatg atgattcgta cgttgatggt gcagccgcct tcagcgaacg
    45841 atcggcaaca ccgcctgcgg gaagtgatgt tttatctcaa cttgtcggag caggaaaaag
    45901 atgcgttttg tgaacgcttc ggcgttcgct tgctgacgtc ttatgggatg acggaaacca
    45961 ttgtgggcat tatcggcgat cgccctggcg ataaacgacg ctggccgtcg attggtcggg
    46021 cggggttttg ctacgaagcg gagatccgcg acgatcacaa tcgcccgctc ccggcaggtg
    46081 agatcggtga aatctgtatt aaaggcgtac cagggaaaac catcttcaaa gagtattttc
    46141 tcaacccgaa agccactgca aaagtgctgg aagctgatgg ctggctgcat accggcgata
    46201 ccggataccg cgacgaagaa ggcttttttt atttcgtcga tcgccgctgc aatatgatca
    46261 aacgcggtgg cgagaatgtc tcctgcgtgg agctggaaaa tatcatcgcc acccatccga
    46321 aaattcagga catcgtggtt gtgggtatta aagattcgat tcgcgatgaa gccatcaaag
    46381 catttgtggt gctgaatgaa ggtgaaacat tgagcgaaga ggaatttttt cgcttctgcg
    46441 aacaaaatat ggcgaaattt aaagtgccct cttatctgga gatcagaaaa gatctgccac
    46501 gtaattgctc ggggaaaata attagaaaga atctgaaata aatgaaacgg cagggaacta
    46561 ccctgccagc taataatcac gttctaaaac aatatgcatt ttttgcaggg atgttgtcgt
    46621 ccctgaaaaa gcaaaaatgg agaaaaggaa tgagtgaatc attacatctg acccgcaatg
    46681 gatcaattct ggaaattacc cttgatcgcc caaaagcgaa tgctattgat gcaaaaacca
    46741 gctttgaaat gggcgaagta tttctaaatt tccgtgacga tccgcaatta cgtgtcgcca
    46801 ttattaccgg tgccggagag aaattctttt ccgcgggctg ggatttaaaa gcggcagcag
    46861 aaggcgaagc accggatgct gactttggcc cgggtggttt tgcgggctta accgaaattt
    46921 tcaatctcga caaaccggtt atcgcagctg tgaacggcta cgcctttggc ggcggtttcg
    46981 aactggcgct ggcggcagat tttattgttt gtgccgataa cgccagcttc gccctgccgg
    47041 aagccaaact gggcatcgtt cctgacagcg gcggtgtgct gcgtctgccg aagatcctgc
    47101 cgcctgccat cgtcaatgaa atgatgatga ccggcagacg aatgggcgca gaagaggcgc
    47161 tgcgttgggg gatagtcaac cgcgtggtta gccaggcgga actgatggat aatgcccgcg
    47221 aactggctca gcagctggtt aacagcgccc cgctggcgat tgcggcgctg aaagagatct
    47281 accgcaccac cagcgaaatg ccggtagaag aagcgtatcg ctatattcgc agcggcgtgt
    47341 tgaaacacta tccatcggtt ctgcattcgg aagatgccat tgaagggccg ctggcgtttg
    47401 ccgagaagcg cgatccggtg tggaaaggac gttaacaacc gtgagctatt acgcctttga
    47461 ggggttaatt ccggtggttc acccgacggc gtttgtccat cccagtgcag tcttgattgg
    47521 cgatgtgatt gtgggagccg gtgtctacat tggcccactc gcctcactgc gtggtgacta
    47581 cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta tgcatggcta
    47641 ctgcgacact gacactatcg ttggggaaaa cggccatatc gggcacggag cgatcctgca
    47701 tggttgtgtg attggtcacg atgcattggt cgggatgaac agcgtgatta tggatggtgc
    47761 ggtcattggc gaagagagca ttgttgccgc catgagcttt gtcaaagcgg gctttcgcgg
    47821 cgagaaacgt cagttgttga tgggtacgcc agcccgcgcg gtacgcactg ttagtgacga
    47881 cgagttacac tggaaacggt tgaataccaa agagtatcag gatcttgtcg ggcgctgtca
    47941 tgcatcgtta catgaaacac agccactgag gcaaatggag gaaaatcgcc cccgtttgca
    48001 ggggacgacg gatgtgacgc ctaaacggtg atcttgccgg atgcggccta aacgccttat
    48061 ccaactctgc tggagagcaa ccgtaggccg gataagatgc gccagcatcg catccggcga
    48121 ccaggtcgca cggtatcatc gaaatgacat taacgacgca tactctttga caacatcatc
    48181 tgccacttct gctcgcggtt aagttccgtg agcggaacgg caggaatacg cggtgtcttt
    48241 cttacgccga ctaatttttc ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg
    48301 atatcgacca ctttaaccag tcgctgacac tggcatcccc gcccttccag cttattaggg
    48361 atcatcttaa cttcgcagct tatttctgtg acttccgaca gaatataagt cagggtatta
    48421 accgccttgc tgtgttcaat atcgaaatga atagagatct ctcttgctat cacccaccga
    48481 ttttcagcca tcatccattc ggcgattaac aagtagagtg gtttttcaac atatccttca
    48541 cacatacgaa ctccagtttc acaaattaaa agtctggcta tgactcgttt tgaagcaatt
    48601 aaacaaggcc atattaaaat tgtggatata tcgatagtat gcaacttcac cgtcgataag
    48661 tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat
    48721 gggcaaaaaa aacggtcctc atcagagaac cgtattagtt attcgatcaa attagcggat
    48781 gaaaaatatc cgccatgaca cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa
    48841 ttactttttc ggtcgcatcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg
    48901 tggtgtcata atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa
    48961 tcgcgcgacg acctgaagtg gtgttgatga tgtaggtata ttcgccattc ttgatgcggt
    49021 cctgaatgtg cggacgacct tcatgcacct tgtttaccag acgcggattg atacccgctt
    49081 cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt
    49141 ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc agcgcacgac
    49201 cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct tcagcgaagg
    49261 tgcggcccac gcccatgact tccccggtag agcgcatttc tggccctaac agcgggtcaa
    49321 cgcccgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac ggcgggataa
    49381 cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc gccgccactt
    49441 ttgccagcgg tacgccggtg gctttggaga cgaacggaac ggtacgcgcc gcacgcgggt
    49501 taacttcaat caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc atcaggccgc
    49561 gcacctgcaa ttcgaaggcc agtttctgca cctgctggcg catcacatcc tgaatttcct
    49621 gacttaaggt gtaggctggc agagaacatg cggagtcacc ggagtgcacg cccgcctgct
    49681 cgatgtgctc cataatgccg ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt
    49741 caacttctac tgcgtcatca aggaaatgat ccagcaacac tggcgcatcg ttagacacgc
    49801 tgaccgccgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt tccatcgccc
    49861 gaccgccgag aacgtaagac ggacgtacca ccagcgggta gccaatctct ttcgccttct
    49921 ctaccgccat ttcaatagcg gtaacggtgg cgttcgccgg ttgtttcagt ttcagacgct
    49981 caaccgcatg ctggaagcgt tcacggtctt ctgcacggtc gatagcatcc gggctggtgc
    50041 cgataaccgg tacgccagca gcttccagcg cgcgcgccag tttcagcggg gtctgaccgc
    50101 cgtactggac gataacgcct ttcggcttct cgatacgcac gatttccagc acatcttcca
    50161 gagttaccgg ctcgaagtag aggcggtcgg aagtgtcgta gtcggtagag acggtttccg
    50221 ggttacagtt aaccataatg gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta
    50281 cgcagcagta gtcgaattcg ataccctgac cgatacggtt tggaccgccg ccaagcacca
    50341 tgattttttc acggtcggta gacggattcg cttcgcactc ttcttcataa gtggagtaca
    50401 tgtaagcggt gtcggtggca aactctgccg cacaggtatc cacgcgcttg tagaccgggt
    50461 gcaggtcata ctggtcacgc agcttacgga tttccgcttc gcgtacgccc gccagttttg
    50521 ccaagcgcgc atcggcaaag cctttgcgtt tcagctggcg caggaattca gcgttcaggc
    50581 cagtgatgcc cacttccgcc actttctctt ccagacgcac cagttcttca atctgtacta
    50641 ggaaccagcg gtcaatgttg gtcaggttga agacgccgtc cacagacagg cccgcacgga
    50701 aagcatcggc gatgtaccag atacgctctg ctcctgcgtc tttcagttcg cgacggattt
    50761 tggttaacgc ttccgggtca tccaggctca ctttcgggtc gaatccagtc gcaccgactt
    50821 ccaggccgcg cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc gccatcactt
    50881 cgccaaccga tttcatctga gtggtcagac ggtcgttagc accggcgaat ttttcgaagt
    50941 tgaagcgagg aattttagta accacatagt cgatggacgg ctcgaaggag gccggagtac
    51001 gtccgccagt gatgtcgttc atcagttcgt cgagggtgta acccaccgcc agtttcgccg
    51061 ccactttagc aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc
    51121 gcgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca aactggacgt
    51181 tggaaccccc ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg ttacgcatga
    51241 tttgatattc tttgtcggtc agcgtttggg ctggcgcgac agtgatggag tcaccggtgt
    51301 ggatgcccat cgcatcgaag ttttcgatag agcagacgat gatgcagttg tcgtttttat
    51361 cacgcaccac ttccatctcg tactctttcc agccgatcag cgactcatca atcagcaact
    51421 ctttggtcgg agagagatcc agaccgcggg cgcaaatttc ttcaaactct tcacggttat
    51481 aagcgatacc gccgccgcta ccgcccatgg taaaggatgg gcgaataatg cacgggaagc
    51541 ccacgtcagc ggcaaccgcc agcgcttctt ccatcgtgtg cgcgataccg gaacgcgcag
    51601 tttccagacc aattttcttc atcgctacgt cgaaacgacg gcggtcttct gctttatcaa
    51661 tcgcatcggc agtggcacca atcatggtga cgccgaactc ttccaacacg ccctgacgtt
    51721 ccagctccag cgcgcagttc agcgccgtct gaccgcccat cgttggcagc accgcgtccg
    51781 ggcgctcttt ttcaataatc ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg
    51841 catcggccat ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc
    51901 ggtaaccctc ttcgcgcagg gctttacatg cttgcgcgcc agagtagtca aactcacacg
    51961 cctgaccgat aacaatcggg cccgcaccca gaatcaggat actttttata tctgtacgtt
    52021 ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct caattaactc
    52081 gataaagtgg tcgaacaacg gcgcggcgtc gtgtggacca gggctggctt cagggtgacc
    52141 ctggaagctg aacgccggtt tatcggtgcg atgaatgccc tgtaacgtac cgtcgaacag
    52201 ggatttatgc gtgacacgca ggtttgcagg taatgttgct tcatccaccg caaaaccgtg
    52261 gttctgggcg gtgatcatca ccacgttttt ctccacatct ttaaccggat ggttgccgcc
    52321 gtggtgacca aatttcattt tgacagtctt cgcaccgctc gccagcgcca gcagctgatg
    52381 accgagacag atgccgaata ccggaatatc ggtttcgagg aatttctgga tggcggtaat
    52441 ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatgc cgtctggatt
    52501 cattttcagc acatcttccg cagaagtttg cgccggaacg atggtcaggc gacagcctct
    52561 atccaccagc atccgcagga tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa
    52621 cggcagctcg tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc agctcccttg
    52681 tgtccagcta taggcttctg cggtggtcac ttcttttgcc agatccatgc cgttcagacc
    52741 tgggaacgcg cgggcttttt ctaacgccag cgccgcatcc gggttatcgc ctgcgataat
    52801 gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg tatcgatatc
    52861 ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg tattacggaa
    52921 gttgctggca atcagcggca gatcgcgaat caccagacct tgtgcatgta cctgagaaga
    52981 ttcttcatcg gcgtcattag tgccgacatt gccaatatgg ggataagtaa gagtaacgat
    53041 ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg aagtattgaa
    53101 aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa actgggttcc
    53161 gtcttccaga accaatagcg ctgacttaat caaaacaccc tccagagaat attcactcac
    53221 tttatttgca tattaattca caatggtgac gtaaatcaat gcaaatctgc ttaccgacga
    53281 atttctggca aacggcggca ttctggagat atgggcgcta aaagtcaact taatggcggg
    53341 attttttata ttattttgtg gctcatgcat gttttacagg cattaagcga caaaaagatc
    53401 atctaacctg ttctggaaaa cgcttgcgca gcaagaagat tgcatgaagt aaaaacgagt
    53461 agaaaaaagt ggaccaaatg
//
LOCUS       DAZFFG010000019        50387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFFG010000019 DAZFFG010000000
VERSION     DAZFFG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.019"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(87..1451)
                     /gene="rhlE"
                     /locus_tag="ACHJ7J_001472"
     CDS             complement(87..1451)
                     /gene="rhlE"
                     /locus_tag="ACHJ7J_001472"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO4999724.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQLRRGEGGAKSSSAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            1680..2351
                     /gene="cecR"
                     /locus_tag="ACHJ7J_001473"
     CDS             1680..2351
                     /gene="cecR"
                     /locus_tag="ACHJ7J_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO4999725.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2354..3349
                     /gene="hlyD"
                     /locus_tag="ACHJ7J_001474"
     CDS             2354..3349
                     /gene="hlyD"
                     /locus_tag="ACHJ7J_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO4999726.1"
                     /translation="MKKTVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3342..5078
                     /gene="ybhF"
                     /locus_tag="ACHJ7J_001475"
     CDS             3342..5078
                     /gene="ybhF"
                     /locus_tag="ACHJ7J_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4999727.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5071..6204
                     /gene="ybhS"
                     /locus_tag="ACHJ7J_001476"
     CDS             5071..6204
                     /gene="ybhS"
                     /locus_tag="ACHJ7J_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4999728.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6215..7321
                     /gene="ybhR"
                     /locus_tag="ACHJ7J_001477"
     CDS             6215..7321
                     /gene="ybhR"
                     /locus_tag="ACHJ7J_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4999729.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTYQTVPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7283..7693)
                     /gene="ybhQ"
                     /locus_tag="ACHJ7J_001478"
     CDS             complement(7283..7693)
                     /gene="ybhQ"
                     /locus_tag="ACHJ7J_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO4999730.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7826..8587
                     /gene="ybhP"
                     /locus_tag="ACHJ7J_001479"
     CDS             7826..8587
                     /gene="ybhP"
                     /locus_tag="ACHJ7J_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4999731.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDDAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8584..9825
                     /gene="clsB"
                     /locus_tag="ACHJ7J_001480"
     CDS             8584..9825
                     /gene="clsB"
                     /locus_tag="ACHJ7J_001480"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO4999732.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALIGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9825..10781
                     /gene="ybhN"
                     /locus_tag="ACHJ7J_001481"
     CDS             9825..10781
                     /gene="ybhN"
                     /locus_tag="ACHJ7J_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO4999733.1"
                     /translation="MSKSHPRWRLAKKILTWLFFITVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWVLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10817..11530)
                     /gene="ybhM"
                     /locus_tag="ACHJ7J_001482"
     CDS             complement(10817..11530)
                     /gene="ybhM"
                     /locus_tag="ACHJ7J_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4999734.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11736..12440)
                     /gene="ybhL"
                     /locus_tag="ACHJ7J_001483"
     CDS             complement(11736..12440)
                     /gene="ybhL"
                     /locus_tag="ACHJ7J_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4999735.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRIFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12576..13028)
                     /gene="moaE"
                     /locus_tag="ACHJ7J_001484"
     CDS             complement(12576..13028)
                     /gene="moaE"
                     /locus_tag="ACHJ7J_001484"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO4999736.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13030..13275)
                     /gene="moaD"
                     /locus_tag="ACHJ7J_001485"
     CDS             complement(13030..13275)
                     /gene="moaD"
                     /locus_tag="ACHJ7J_001485"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO4999737.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13268..13753)
                     /gene="moaC"
                     /locus_tag="ACHJ7J_001486"
     CDS             complement(13268..13753)
                     /gene="moaC"
                     /locus_tag="ACHJ7J_001486"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO4999738.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13756..14268)
                     /gene="moaB"
                     /locus_tag="ACHJ7J_001487"
     CDS             complement(13756..14268)
                     /gene="moaB"
                     /locus_tag="ACHJ7J_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO4999739.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14290..15279)
                     /gene="moaA"
                     /locus_tag="ACHJ7J_001488"
     CDS             complement(14290..15279)
                     /gene="moaA"
                     /locus_tag="ACHJ7J_001488"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO4999740.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15267..15411)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15676..16584
                     /gene="yvcK"
                     /locus_tag="ACHJ7J_001489"
     CDS             15676..16584
                     /gene="yvcK"
                     /locus_tag="ACHJ7J_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO4999741.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16775..18796)
                     /gene="uvrB"
                     /locus_tag="ACHJ7J_001490"
     CDS             complement(16775..18796)
                     /gene="uvrB"
                     /locus_tag="ACHJ7J_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO4999742.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19375..20052)
                     /gene="bioD"
                     /locus_tag="ACHJ7J_001491"
     CDS             complement(19375..20052)
                     /gene="bioD"
                     /locus_tag="ACHJ7J_001491"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4999743.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLLLDYATVNPYTFAEPTSPHIISAQEGRPIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20045..20800)
                     /gene="bioC"
                     /locus_tag="ACHJ7J_001492"
     CDS             complement(20045..20800)
                     /gene="bioC"
                     /locus_tag="ACHJ7J_001492"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO4999744.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLVMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20787..21941)
                     /gene="bioF"
                     /locus_tag="ACHJ7J_001493"
     CDS             complement(20787..21941)
                     /gene="bioF"
                     /locus_tag="ACHJ7J_001493"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO4999745.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFFHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLGEIQQVTQQHNGWLMVDDAHGTGVIGDQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21938..22978)
                     /gene="bioB"
                     /locus_tag="ACHJ7J_001494"
     CDS             complement(21938..22978)
                     /gene="bioB"
                     /locus_tag="ACHJ7J_001494"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO4999746.1"
                     /translation="MAHRPRWTLSQVTELFDKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23065..24354
                     /gene="bioA"
                     /locus_tag="ACHJ7J_001495"
     CDS             23065..24354
                     /gene="bioA"
                     /locus_tag="ACHJ7J_001495"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO4999747.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24413..24889
                     /gene="ybhB"
                     /locus_tag="ACHJ7J_001496"
     CDS             24413..24889
                     /gene="ybhB"
                     /locus_tag="ACHJ7J_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO4999748.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25041..26324
                     /gene="ybhC"
                     /locus_tag="ACHJ7J_001497"
     CDS             25041..26324
                     /gene="ybhC"
                     /locus_tag="ACHJ7J_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO4999749.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(26465..28726)
                     /gene="ybhJ"
                     /locus_tag="ACHJ7J_001498"
     CDS             complement(26465..28726)
                     /gene="ybhJ"
                     /locus_tag="ACHJ7J_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO4999750.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(28909..30342)
                     /gene="ybhI"
                     /locus_tag="ACHJ7J_001499"
     CDS             complement(28909..30342)
                     /gene="ybhI"
                     /locus_tag="ACHJ7J_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO4999751.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(30418..31470)
                     /gene="ybhH"
                     /locus_tag="ACHJ7J_001500"
     CDS             complement(30418..31470)
                     /gene="ybhH"
                     /locus_tag="ACHJ7J_001500"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO4999752.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIIPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            31654..32607
                     /gene="ybhD"
                     /locus_tag="ACHJ7J_001501"
     CDS             31654..32607
                     /gene="ybhD"
                     /locus_tag="ACHJ7J_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4999753.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFS
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(32648..33643)
                     /gene="pgl"
                     /locus_tag="ACHJ7J_001502"
     CDS             complement(32648..33643)
                     /gene="pgl"
                     /locus_tag="ACHJ7J_001502"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO4999754.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            33792..34610
                     /gene="ybhA"
                     /locus_tag="ACHJ7J_001503"
     CDS             33792..34610
                     /gene="ybhA"
                     /locus_tag="ACHJ7J_001503"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO4999755.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(34611..35669)
                     /gene="modC"
                     /locus_tag="ACHJ7J_001504"
     CDS             complement(34611..35669)
                     /gene="modC"
                     /locus_tag="ACHJ7J_001504"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO4999756.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(35672..36361)
                     /gene="modB"
                     /locus_tag="ACHJ7J_001505"
     CDS             complement(35672..36361)
                     /gene="modB"
                     /locus_tag="ACHJ7J_001505"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO4999757.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     SLLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLVSEW
                     LARISRERAGR"
     gene            complement(36361..37134)
                     /gene="modA"
                     /locus_tag="ACHJ7J_001506"
     CDS             complement(36361..37134)
                     /gene="modA"
                     /locus_tag="ACHJ7J_001506"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4999758.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(37301..37450)
                     /gene="acrZ"
                     /locus_tag="ACHJ7J_001507"
     CDS             complement(37301..37450)
                     /gene="acrZ"
                     /locus_tag="ACHJ7J_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO4999759.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            37579..38367
                     /gene="modE"
                     /locus_tag="ACHJ7J_001508"
     CDS             37579..38367
                     /gene="modE"
                     /locus_tag="ACHJ7J_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO4999760.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            38435..39907
                     /gene="modF"
                     /locus_tag="ACHJ7J_001509"
     CDS             38435..39907
                     /gene="modF"
                     /locus_tag="ACHJ7J_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO4999761.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            40169..41185
                     /gene="galE"
                     /locus_tag="ACHJ7J_001510"
     CDS             40169..41185
                     /gene="galE"
                     /locus_tag="ACHJ7J_001510"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO4999762.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            41195..42241
                     /gene="galT"
                     /locus_tag="ACHJ7J_001511"
     CDS             41195..42241
                     /gene="galT"
                     /locus_tag="ACHJ7J_001511"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO4999763.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            42245..43393
                     /gene="galK"
                     /locus_tag="ACHJ7J_001512"
     CDS             42245..43393
                     /gene="galK"
                     /locus_tag="ACHJ7J_001512"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO4999764.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTEISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNNFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            43387..44427
                     /gene="galM"
                     /locus_tag="ACHJ7J_001513"
     CDS             43387..44427
                     /gene="galM"
                     /locus_tag="ACHJ7J_001513"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO4999765.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLLPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGNGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            44637..45389
                     /gene="gpmA"
                     /locus_tag="ACHJ7J_001514"
     CDS             44637..45389
                     /gene="gpmA"
                     /locus_tag="ACHJ7J_001514"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO4999766.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(45547..46599)
                     /gene="aroG"
                     /locus_tag="ACHJ7J_001515"
     CDS             complement(45547..46599)
                     /gene="aroG"
                     /locus_tag="ACHJ7J_001515"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO4999767.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            46915..47295
                     /gene="ybgS"
                     /locus_tag="ACHJ7J_001516"
     CDS             46915..47295
                     /gene="ybgS"
                     /locus_tag="ACHJ7J_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO4999768.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            47409..48350
                     /gene="zitB"
                     /locus_tag="ACHJ7J_001517"
     CDS             47409..48350
                     /gene="zitB"
                     /locus_tag="ACHJ7J_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO4999769.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(48347..49066)
                     /gene="pnuC"
                     /locus_tag="ACHJ7J_001518"
     CDS             complement(48347..49066)
                     /gene="pnuC"
                     /locus_tag="ACHJ7J_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO4999770.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALTWLAVCVIAIGLMTVFINPVFAFLTRVAVIIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(49104..50147)
                     /gene="nadA"
                     /locus_tag="ACHJ7J_001519"
     CDS             complement(49104..50147)
                     /gene="nadA"
                     /locus_tag="ACHJ7J_001519"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO4999771.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIERLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT        12217 a        13360 c        12841 g        11969 t
ORIGIN      
        1 ataaaatcag acatgtcatg catgtctgac cgtgttacct gaataatggg caaaagcctg
       61 tgatactctt ttgcccttac atggaattac tgcgcagcgg caggtttacg cggacggcgg
      121 cgacgttgtt gttcgcctgc cggtttggca tcgccgaggc ggcgagacgg tttttctgca
      181 ggttttgcgc ttgaagattt tgcgccacct tctcctcggc gtagttgctg ttgaccgcga
      241 ccaccgccct gcccacgacc accgccgcca cgttgctggc gaccgttctg gatcggttcg
      301 gctttgattg acgggtccgg ctcatagccc ggaatcgcaa tgcgcgggat ctcttttttc
      361 agcagttttt cgatatcacg cagcagtttg tgttcatcaa cacacaccaa cgacaacgct
      421 tctccggtgg ctgcggcacg accggtacgc ccgatacggt ggacataatc ttccggtacg
      481 tttggcagtt catagttgac gacgtgcggc aactcttcga tatccagacc gcgcgcagcg
      541 atgtcagttg ccaccagtac acgaatatcg cccgatttaa aatcagccag cgcacgggta
      601 cgcgcacctt gcgatttatt accgtggatc gccgcactac ggatgccatc tttattaagc
      661 tgttcagcga gatggttagc gccatgtttg gtacgggtaa acaccagcac ctgctgccag
      721 ttccctttcc caatcatgtg cgacagcaat tcgcgtttgc gcttcttatc aacaaagtga
      781 acatgctggg tcacctgatc agacgcggta ttgcggcgtg ccacttcgat ttccagcggg
      841 ttgtgcaaca gtttttccgc cagggcttta atatcgtcag agaaggtcgc ggagaacaac
      901 aggttctggc gcttcgcagg tagttttgtt aacacgcgac ggatatcgtg gataaagccc
      961 atgtcgagca tgcggtcagc ttcatcgagg acgaggattt caacctgatc cagcttcact
     1021 gcattctgat gttccaggtc cagcaaacgt cccggcgttg ccaccagcac atcaacgccg
     1081 ccgcgcaact tcatcatttg tgggttaatg ctgacaccgc caaacaccac cagcgaacga
     1141 atgttcagat atttgctgta atcacggacg ttttcgccaa tctgcgcagc cagttcacgg
     1201 gtcggggtaa gaatgagagc acgtaccgga cgacgccctt tggcgtgcgg ctggcgagtg
     1261 atcaggtgtt gcaacagcgg cagcgtaaag cccgctgttt tgccggtgcc ggtctgggcg
     1321 ctagccatca ggtcgcggcc ttccagcacc gcagggatcg cctgctgctg aataggggtg
     1381 ggttcacggt aaccctgctc ggcaacggcg cgcaggatat cagggcttaa acccaaagaa
     1441 tcgaaagaca taactactcc gaaccgcccc gaccgttacc ggtgtagttt tcagggagat
     1501 actgaaaagg aaaatgacaa aaaccacaat gtcatgaggg agcggagtgt agcagttttt
     1561 gtgacgcagc gcataaatta tccctgtgac tggcgtagct aaaattctta gccatactgg
     1621 acaactcccc tatggagtca taatcttaat caatcatttg attaagaatg gactgtgcga
     1681 tgaataatcc tgccatgaca atcaagggtg aacaggcgaa aaaacagctg attgctgccg
     1741 cactggcgca gtttggtgaa tatggaatga acgccaccac ccgcgagata gccgcccagg
     1801 ccgggcagaa tatcgctgcc atcacctact acttcggttc gaaagaagat ttgtacctcg
     1861 cctgcgccca gtggattgcc gattttattg gcgagcagtt ccgtccgcat gccgaggaag
     1921 ccgaacgctt gttcgcccaa ccacagcctg atcgggctgc catccgtgaa ctgatccttc
     1981 gcgcctgcag gaacatgatt aagctgctca cccaggatga caccgtcaac ctcagcaagt
     2041 ttatctcccg tgagcagctc tctcccacgg cagcctacca cctggtgcac gaacaggtga
     2101 ttagtccgct acacagccac ctgacacgcc tgattgccgc ctggaccggc tgcgacgcca
     2161 atgacacccg catgatcctt catacccatg cgttgattgg tgagattctg gcgttccgtc
     2221 ttggcaaaga aacaattctg ttacgtaccg gctggaccgc gttcgatgaa gaaaagaccg
     2281 aactgatcaa ccagacggtg acctgtcata tcgacctgat tttgcaagga ttatcgcaaa
     2341 ggagtttgta gtgatgaaaa aaactgtcgt gatcggactg gcggtagtgg tacttgccgc
     2401 cgtggttgcc ggaggctact ggtggtatca aagccgccag gataacggcc tgacgctgta
     2461 tggcaacgtg gatattcgta cggtgaatct tagtttccgt gttggggggc gcgttgaatc
     2521 gctggcggtg gacgaaggtg atgcgatcaa agcgggccag gtgctgggcg aactggatca
     2581 caagccgtat gagattgccc tgatgcaggc gaaagcgggc gtctcggtgg cacaggcgca
     2641 gtatgacctg atgcttgccg ggtatcgcga tgaagaaatc gctcaggccg ccgcagcggt
     2701 gaaacaggcg caagccgcct atgactatgc gcagaacttc tataaccgcc agcaaggact
     2761 gtggaaaagc cgcaccattt cggcaaatga cctggaaaat gcccgctcct cgcgcgacca
     2821 ggcacaggca acgctgaaat cagcacagga taaattgcgt cagtaccgtt ccggtaaccg
     2881 tgaacaggac atcgctcagg cgaaagccag cctcgaacag gcgcaggcgc aattggcgca
     2941 ggcggagctg aatttacagg actcaacgtt gatagcaccg tctgatggca cgctgttaac
     3001 gcgcgcggtg gagccaggta cggtcctcaa tgaaggtggc acggtgttta ccgtttcact
     3061 aacgcgtccg gtgtgggtgc gcgcttatgt tgatgaacgt aatcttgacc aggcccagcc
     3121 ggggcgcaaa gtgctgcttt ataccgatgg tcgcccggac aagccttatc acgggcagat
     3181 tggtttcgtt tcgccgactg ctgaatttac cccgaaaacc gtcgaaacgc cggatctgcg
     3241 taccgacctc gtctatcgcc tgcgtattgt agtgaccgac gccgatgatg cgttacgcca
     3301 gggaatgcca gtgacagtac aattcggtga cgaggcagga catgaatgat gccgttatca
     3361 cgctgaacgg cctggaaaaa cgctttccgg gcatggacaa gcccgccgta gcgccactcg
     3421 actgtaccat tcatgccggt tatgtgacgg ggttggtggg gccggatggc gcaggcaaaa
     3481 ccacgctgat gcggatgctc gccggattac tgaaacccga cagtggcagt gccacggtga
     3541 ttggctttga tccgatcaaa aacgacggcg cgctgcacgc cgttctcggc tatatgccgc
     3601 agaaattcgg tctgtatgaa gatctcaccg tgatggagaa tctcaatctg tacgcggatt
     3661 tgcgcagcgt caccggtgaa gcgcggaagc aaacttttgc tcgcctgctg gagtttacgt
     3721 ctcttggccc gtttaccgga cgcctggcgg gcaagctctc cggtgggatg aaacaaaaac
     3781 tcggcctggc ctgtaccctg gtgggcgaac cgaaagtgtt gctgctcgat gaacccggcg
     3841 tcggcgttga ccctatctca cggcgcgaac tgtggcagat ggtgcatgag ctggcaggcg
     3901 aagggatgtt aatcctctgg agtacctcgt atctcgacga agccgagcag tgccgtgacg
     3961 tgttactgat gaacgaaggc gagttgctgt atcagggaga accaaaagcc ctgacacaaa
     4021 ccatggccgg acgcagcttt ctgatgacca gtccacacga gggcaaccgc aaactgttgc
     4081 aacgcgcctt gaaactgccg caggtcagcg acggcatgat tcaggggaaa tcggtacgtc
     4141 tgatcctcaa aaaagaggcc acaccagacg acattcgcca tgccgacggg atgccggaaa
     4201 tcaacatcaa cgaaactacg ccgcgttttg aagatgcgtt tattgatttg ctgggcggtg
     4261 ccggaacctc agaatcgccg ctgggtgcaa tattgcatac ggtagaaggc acacccggcg
     4321 agacggtgat cgaagcgaaa gaactgacca agaaatttgg tgattttgcc gccaccgatc
     4381 acgtcaactt tgccgttaaa cgcggggaga tttttggttt gctggggcca aacggcgcgg
     4441 gaaaatcgac cacctttaag atgatgtgcg gtttgctggt gccgacttcc ggccaggcgc
     4501 tggtgctggg gatggatctg aaagagagtt ccggtaaagc gcgccagcat ctcggctata
     4561 tggcgcaaaa attttcgctc tacggcaacc tgacggtcga acagaattta cgctttttct
     4621 ctggtgtgta tggcttacgc ggtcgggcgc agaacgaaaa aatctcccgt atgagcgagg
     4681 catttggcct gaaaagtatt gcctcacacg ccaccgatga actgccatta ggttttaaac
     4741 agcggctggc gctggcctgt tcgctgatgc atgaaccgga tattctgttt ctcgacgaac
     4801 cgacttccgg cgttgacccc ctcacccgcc gtgaattctg gctgcacatc aacagcatgg
     4861 tagagaaagg cgtcacggtg atggtcacca cccactttat ggatgaagcg gaatattgcg
     4921 accgcatcgg cctggtgtac cgcgggaaat taatcgccag cggcacgccg gacgatttga
     4981 aagcacagtc ggctaacgat gagcaacccg atcccaccat ggagcaagcc tttattcagt
     5041 tgatccacga ctgggataag gagcatagca atgagtaacc cgatcctttc ctggcgtcgc
     5101 gtgcgggcgc tgtgcgtcaa agagacgcgg cagatcgttc gcgatccgag tagctggctg
     5161 attgcggtag tgatcccgct gttgctgctg tttatttttg gttacggcat taacctcgac
     5221 tccagcaagc tgcgggtcgg gattttactg gaacagcgta gcgaagcggc gctggatttc
     5281 acccacacta tgaccggttc gccctacatc gacgccacca tcagcgataa ccgtcaggaa
     5341 ctgatcgcca aaatgcaggc ggggaaaatt cgcggtctgg tggttattcc ggtggatttt
     5401 gcggaacaga tggagcgcgc caacgccacc gcaccgattc aggtgatcac cgacggcagt
     5461 gagccgaata ccgctaactt tgtacagggg tatgtcgaag ggatctggca gatctggcaa
     5521 atgcagcgag cggaggacaa cgggcagact tttgaaccgc ttattgatgt acaaacccgc
     5581 tactggttta acccggcggc gataagccag cactttatta tccccggtgc ggtgaccatc
     5641 atcatgacgg tcattggcgc gattctcacc tcgctggtgg tggcgcggga gtgggaacgc
     5701 ggcactatgg aggctttgct ctctacggag attacccgca cggaactgct gctgtgtaag
     5761 ctgatccctt attactttct cgggatgctg gcgatgttgc tgtgtatgct ggtgtcagtg
     5821 tttattctcg gcgtgccgta tcgcggttcg ctgctgattc tgttttttat ctccagcctg
     5881 tttttactca gtaccctggg gatggggctg ctgatttcca cgattacccg caaccagttc
     5941 aatgccgctc aggtcgccct gaacgccgcg ttcctgccgt cgattatgct ttccggcttt
     6001 atttttcaga tcgacagtat gcccgcggtg atccgcgcgg tgacgtacat tattcccgct
     6061 cgttatttcg tcagcaccct gcaaagcctg ttcctcgccg ggaatattcc agtggtgctg
     6121 gtggtaaacg tgctgttttt gatcgcttcg gcggtgatgt ttatcggcct gacgtggctg
     6181 aaaaccaaac gtcggctgga ttagggagaa gagcatgttt catcgcttat ggacgttaat
     6241 ccgcaaagag ttgcagtcgt tgctgcgcga accacaaacc cgcgcgattc tgattttacc
     6301 cgtgctaatt caggtgatcc tgttcccgtt cgccgccacg ctggaagtga ctaacgccac
     6361 catcgccatc tacgatgaag ataacggcga gcattcggtg gaacttactc aacgttttgc
     6421 ccgcgccagc gcctttacac atgtgctgct gctgaaaagc ccacaggaga tccgccccac
     6481 catcgacaca caaaaggcgt tactgctggt gcgtttcccg gctgacttct cgcgcaaact
     6541 ggatacctac cagaccgtgc cgttgcagtt gatcctcgac gggcgtaact ccaacagtgc
     6601 gcaaattgcc gccaactacc tgcaacagat cgtcaaaaat tatcagcagg aactgctgga
     6661 aggaaaaccg aaacccaaca acagcgagct ggtggtacgc aactggtata acccgaatct
     6721 cgactacaaa tggtttgtgg tgccgtcgct gatcgccatg atcaccacta tcggcgtaat
     6781 gatcgtcact tcactttccg tcgcccgcga acgtgaacaa ggtacgctcg atcagctact
     6841 ggtttcgccg ctcaccacct ggcagatctt catcggcaag gccgtaccgg cattaattgt
     6901 cgccaccttc caggccacca ttgtgctggc gattggtatc tgggcgtatc aaatcccctt
     6961 cgccggatcg ctggcgctgt tctactttac gatggtgatt tatggtttat cgctggtggg
     7021 attcggtctg ttgatttcat cactctgttc aacacaacag caggcgttta tcggcgtgtt
     7081 tgtctttatg atgcccgcca ttctcctttc cggttacgtt tcgccggtgg aaaacatgcc
     7141 agtatggctg caaaacctga cgtggattaa tcctattcgc cactttacgg acattaccaa
     7201 gcagatttat ttgaaggatg cgagtctgga tattgtgtgg aatagtttgt ggccgctact
     7261 ggtgataacg gccacgacag ggtcagcggc gtacgcgatg tttagacgta aggtgatgta
     7321 acttcttatc tttcgccagc aaagacacta ccgccgggcc ggcaaggatt gccagccctg
     7381 caattgccag caaaaagttg ttttccagaa cgcgggacaa cagccacagc acaatcagcg
     7441 ctgcgccaaa ataccaggtc gtggtcagct cttccagcac gtctgccacc tcacgccagc
     7501 gttgctcggg gtggcgctga aacaccagca tcatcactac cgtgacacaa tacagtgcgc
     7561 ataatcccac gccgacgtga accagagtct tactcatcca gcccaccacc agcagcgcca
     7621 ctaccagtaa atgcaacata atctgccagc aacttagacc cgttgcgaca cgaacacgtt
     7681 gttgccactt catggcttct ctcctgaaga atttatctct acttactcag agtaccgaac
     7741 tttacggaaa attccgtaag accgcacctt tttgtgacgc cgactacact atttcaatga
     7801 gataagaggc aaagacagga aaattatgcc cgatcaaaca caacaatttt cgttcaaggt
     7861 gctcaccatc aatattcaca aaggctttac cgcgtttaac cgacgcttca ttttgccgga
     7921 acttcgcgat gccgtgcgta ccgtcagcgc cgatattgtt tgcctgcagg aagtgatggg
     7981 cgcgcacgaa gttcatccgc tgcatgtgga aaactggccc gatacctcgc actacgagtt
     8041 tctcgccgac actatgtgga gcgattttgc ctacggtcgc aatgccgtat acccggaagg
     8101 gcatcacggc aacgccgtac tgtcgcgtta tcccattgaa cattatgaga atcgcgatgt
     8161 ttcggtcgat gatgcggaaa agcgcggcgt gctctactgc cgcattgtgc cgccgatgac
     8221 cggaaaagcg attcatgtga tgtgcgtaca tctgggcctg cgtgaggcgc accgtcaggc
     8281 gcagcttgcg atgctcgccg aatgggtgaa tgagctaccg gacggcgaac cggtattggt
     8341 ggcgggtgat ttcaatgact ggcggcaaaa agctaatcat ccgttaaaag tgcaggccgg
     8401 actggatgag atttttaccc gcgcccacgg acgcccggcg cgcacgtttc cggtgcaatt
     8461 tcctctacta cgactggaca ggatctacgt caaaaatgcc agcgccagcg cgccaaccgc
     8521 gttgccgctg cggacatggc gacacctttc tgatcatgcc cctttaagtg cggagattca
     8581 tttatgaaat gtagctggcg cgaaggcaat aagatccagt tgctggaaaa cggcgagcaa
     8641 tattatcccg cggtgtttaa ggcgattggc gaggcacaag aacgcatcat tcttgaaacg
     8701 tttatctggt ttgaggatga cgtcggcaaa caactacatg cggcactact ggcagcagcg
     8761 caacgcgggg ttaaagcgga agtcttgctg gatggctacg gttcgccgga tctcagcgat
     8821 gagtttgtca atgaactgac ggcagctggc gtagtgttcc gctactacga tccccgccct
     8881 cgcctttttg gtatgcgcac caatgtgttt cgccggatgc atcgcaaaat tgtggtgatc
     8941 gacgcgcgta tagcctttat tggcgggctg aattactccg ccgagcatat gtccagctac
     9001 ggtccagagg ctaaacagga ttacgcggta cgccttgaag ggccgattgt cgaagatatt
     9061 ctccagtttg agctggaaaa cctgcctgga cagagcgcgg cacgacgctg gtggcgacgt
     9121 catcacaaag cggaagagaa ccgccagccg ggagaagcgc aggtattgct ggtctggcgc
     9181 gataacgaag aacatcgcga tgatatcgaa cgtcactatc tgaaaatgct cactcaggcg
     9241 cggcgggaag tgattatcgc caacgcctac ttcttccccg gctatcgatt tttacacgcc
     9301 ttgcgtaaag cggcacggcg cggggtgcgg atcaaactga tcattcaggg cgaaccggat
     9361 atgccgattg tcagagtcgg tgcacgcttg ctgtataact atctggttaa aggtggcgtt
     9421 caggtgtttg agtaccgccg ccgcccgctc cacggcaaag tagcattgat ggacgatcac
     9481 tgggcgacag tagggtccag taatctcgat ccgctcagtt tgtcactgaa tctcgaagca
     9541 aatgtcatca tccacgatcg tcattttaac cagacgctgc gcgataatct gaacggcatt
     9601 atcgccgcag attgtcagca ggtggatgaa accatgctgc ccaaacgcac ctggtggaac
     9661 ctgaccaaaa gcgtgctggc gttccacttt ttacgccact tcccggcgct gattggctgg
     9721 cttccggcac acacaccacg tctggcgcag gttgatcccc ccgcacaacc gacaatggaa
     9781 acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac cctgatgagt aaatcacacc
     9841 cgcgctggcg cttagcaaag aagatcctca cctggctgtt ttttatcacg gtgattgtgt
     9901 tgctggtggt ctacgccaaa aaagtggact gggaagaggt ctggaaggtc atccgcgact
     9961 acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt cgtcagctat ctgatttacg
    10021 gctgctatga cctgctcgcc cgcttttact gcggtcacaa actggcgaag cgccaggtga
    10081 tgctggtgtc gtttatctgc tacgccttca acctgacgct cagtacctgg gtcggcggca
    10141 ttggtatgcg ctatcgtttg tactctcgtc tggggttgcc cggtagcact attacgcgaa
    10201 ttttctcgct cagtattacc accaactggc tgggctatat tttgctggcg gggattatct
    10261 ttaccgcagg cgtagtggaa ctgccggacc actggtatgt cgatcaaacc acgctgcgca
    10321 ttctcggcat tggcttactg atgattatcg cggtttattt gtggttttgc gctttcgcga
    10381 agcaccgcca tatgaccatc aaagggcaaa agctggtgct gccttcatgg aaattcgccc
    10441 tcgctcagat gctgatttcc agcgttaact ggatggtaat gggagcgatt atctgggtgt
    10501 tacttggtca aagcgtgaac tatttctttg tactgggcgt gttgctggtt agtagtattg
    10561 ctggcgtcat cgtgcatatt ccggcgggaa ttggtgtgct ggaagcggtg tttatcgcgc
    10621 tactggctgg ggagcataca tccaaaggca caattatcgc cgccctactc gcttaccgtg
    10681 tgctgtatta ctttatccca ctgctgctgg cgctgatctg ttacctgttg ctggaaagcc
    10741 aggcgaagaa gctacgggcg aaaaatgaag cggcgatgtg aagttcagtt aagttcttta
    10801 gtatgtgcat ttacgactaa tgaaaaaaac gcgtatgcct ttgccagaca agcgttatag
    10861 ctatcgctgc aagaatccca aagaagccga taaaatagta atagagtgtt aatgcacctg
    10921 taacagcgca acgaaccgga ctccacaact cttcactatg gcattttcct gccagttcaa
    10981 ggagcttatt tcgtccatgc gagataatgc ctgaccataa cacaatcatt aagcaactta
    11041 ttacccaaat gggccgttca ctcattaatg ccgcattcac gatgattacc agggccaacc
    11101 ctgtcaacgt catcataatg ataaaacggt gagaaccagg atcaacatta aataataaac
    11161 cggccagcat actgattaaa aacatcgcgc ccgcagtgct gaacacgatg ataatggcat
    11221 atacaggaac taaacttgca acgacaatcg ccaccaaaga gagtaaaaac agatatatta
    11281 cgcctgcgat ggcggcattt atcttgttga tatccttact taagccatac atacaaagta
    11341 ataataaccc tatggcgaca tacattggtg gtgaatcata tttggttgcc gtaatttcga
    11401 gatacttagc accccaggcc agccctgcgg ttgccaccaa acctaaagcg gtaatgagcc
    11461 atataccgtt taatatcctg gtctcgtgct gaaaatctaa tttattgctg ttttgcaagt
    11521 atgattccat tcttttagtt gtccttagaa aaaatattaa tattcaccat caataaattt
    11581 aaacccacgc atacaaaata cttttgtatt tatggatacg gaaaacgctt ccgctcggcc
    11641 ataaggcgta ttttatacct ggtacttgtt aagtgtcgtt tttagtaaga aaaaaaatcc
    11701 cgacacatgg ccgggatatt gatggtgaaa aagaattaac ggcggttgcc aaaaattcgc
    11761 agcaacatca ggaacaggtt gatgaagtcc agatacaagg ttaatgcgcc aagaatggaa
    11821 tatttgcgca ggttcgacgc gtcgcgggta tcaatctgct cacccatgtt tttcagtttc
    11881 tgggtgtcgt atgcggtcag ccccacaaaa acaatcacac cgatataggt gacggcccac
    11941 atcaacgctt cgcttttcag ccagaagttg accagcgatg ccaacacaat gccgattaac
    12001 gccataaaca gcatattgcc aaagccactt aaatcgcgct tcgtggtgta accgtacagg
    12061 ctcattgcgc cgaacatccc ggcagtaacg acgaaggtac tggcgataga agcagcggta
    12121 taaacaatga aaatactgga aagcgtaaga cccgtcagcg ccgaataaag cataaagagc
    12181 atcgtcgtta cacctgcgct cagcttttga atcatcgctg ataacacaat aaccaatgct
    12241 aattgcgcga tgatcagacc gattaaaaag atacggttag tgaacaacag ctccatcacg
    12301 gccgcggaat tagccgcata ccaggcaaca aatgcggtca gcaacaagcc aacggtcatc
    12361 cagccataga cttgcgccat ataagtctgc aagccagccc ggggttgtac aattgaatca
    12421 gaacgtggga atctgtccat gacgatctcc tgaagatata aggaatatct taaggatact
    12481 gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca tgtactgagc ggtgaaattg
    12541 ccggacgcag cggtgcctta tccggctaac aaaaactacc agcgttttgc cgcctgctga
    12601 tcgctctccc gagcttcgac ccagcggtcg ccctccggcg tggcttcgcg cttccagaac
    12661 ggtgcgcggg ttttgagata atccataata aactgcccgg cttcaaacgc actgctgcga
    12721 tgcgcactgg tgacaccgac aaaaacgatt tcatcgcccg gccataattc cccgatgcgg
    12781 tgaatcaccg tgacgcgccc cagcggccag cggttacgcg cttcatcgac aatttctgcc
    12841 agtgcttttt cagtcatccc cggatagtgt tcgagggtta atgctttgac gctgtcgccc
    12901 aggttatggt tgcgcacctt accggtaaag gtgactaccg caccgtcttc gtcacgctcc
    12961 gccagccacg ggtactcttc tcctacgctg aacggctgcg gaccaacaac aattttggtt
    13021 tctgccatct taacctccgg ttaccggcgg gaagaaagct acttcgtcgc cgtcagtcag
    13081 cggatggtca aaactcacca gcgtctggtt gacggcagcc agtaatttgc catcttccag
    13141 cgccagcgcc cagcgatcgc tctgcgcagc caggtgctgg cgtaacgctt caacggttgg
    13201 gaaatccgca gccacttcgg ttgcatctgt tcctaccaac tcgcgcacct gggcgaaaaa
    13261 aagaacttta atcatccgct tccaccttaa agtcacccga cttgccgccg ctcttcgcca
    13321 gcaaacgtac cggaccaatc accatatctt tttgcaccgc tttgcacatg tcataaatgg
    13381 tcagcgccgc cacggaggcc gcggttaacg cttccatttc gacaccggtt ttcccggtca
    13441 ggcggcataa ggtttctata cgcacccgat tgtgctccgg ctcggcctgt aaattgactt
    13501 caactttgct gagcatcagc ggatgacaca gcgggatcag atcccaggtg cgttttgccg
    13561 cctgaatacc ggcaatacgc gcagtggcaa atacgtcgcc tttgtggtgg cgaccatcaa
    13621 taatcatcgc cagcgtctcg ctgcgcatgg tgacaaaggc ttccgcccgc gcttcacgca
    13681 cggtttccgc tttggcggag acatccacca tgtgcgcttc gccagcggcg ttgatatggg
    13741 tcagttgcga catacttatt tcttcaaatg tggatggaaa ttacacggac gcgtacgggc
    13801 atccagctgc ggcgcgatga tattttccca tgcggtacgg cacgctttgg tcgaacccgg
    13861 catggcgaaa atcagcgttt tgttagcgac gcccgctacc gcacgagatt gcaacgtgga
    13921 agtgccaatc tcttcaaacg acaacatacg gaacacttca ccaaaacctt caacttcacg
    13981 gtcgaacaac ggcagcaatg cttcgggagc ctgatcacct tccgtcaggc cagtcccccc
    14041 cgtaatcaac accacttgta catcgtcgct ggcgatccac gcagatacct gagcgcgaat
    14101 agcgtagcgg ttttctttca caatggcttt atcgacaacg tgatggcccg cttcttgcgc
    14161 cgaatcgcgc aaatagtgac cggaggtatc gtcttcttca ccgcgacgat tagaaaccgt
    14221 aagaatagca atacgggtcg ggataaattc agtgcttacc tgactcatct gatctctcct
    14281 tttgacgttt tagccgccaa tgtacgataa gttttgcgta ataccggtgt tgttttgatg
    14341 caggaaatgg gtctgtttct tctcccgcag cgccgctgaa atacgcgctt ccagcgcctg
    14401 ttgctgggta tcgtcttcca gcagatcgcg caggttaacg ccgccttcac caaacaggca
    14461 gagatggagt ttaccaatgg aggaaacgcg cagacggttg caagtggcgc agaagtcttt
    14521 ttcatacggc atgataaggc caatctctcc ggcgtaatcc ggatgacaaa agacttgcgc
    14581 gggaccgtcg ctgcgttgac gtaattggtg gatccagccg cgacgcagta gctcgtcacg
    14641 cagaacctga ccagagatgt gatgcttacg gaagagctcg ctgccctcgc ccgtttccat
    14701 cagttcgatg aaacgcagct ggatagggcg atgctggatc cagttcagaa aggtgtcgag
    14761 ctggtgatga ttcacatcac gcatcagcac ggtattgact ttgaccttct caaaaccggc
    14821 ctcaaatgca gcatcgatcc ctgccatgac ctggttgaat ttatcctgcc cggtaatagc
    14881 gtgaaactgg cgggcgtcca gactatcaac gctgacgttg atgccagtaa gtccagcatc
    14941 gcgccagttc gccacatcgc gttccagacg gtaaccattg gtggtgaccg caatctggcg
    15001 gatagcgtca ttttctcgca cagcggcgat gatatcggta aagtcgcggc gtaaagacgg
    15061 ctcaccacct gtcagacgga ctttttcggt gcccagactg gcgaaggcgc gcgtaacccg
    15121 gcgaatttca tcgacggtca gaaagccttt attggtgacg ccgctcggtt tgtagccatc
    15181 cggcaggcag taggtgcaac gaaagttaca cacatcggta atcgacaggc gcaagtagta
    15241 aaacttacgc gcaaatgcat cagtcagttg tgaagccatg tacacctttc cagatacggg
    15301 aggcgaagtc atttcttcct tcgccctggt ggcaatcttt acgagcattg tcacggccaa
    15361 agcaccgtat cagttgaccc aggtgcagag gctagagtgt ttagtggtta agccgatact
    15421 agcgtgtaaa tgagattttt accatctaca ctttcgctat gtaatcatgt atatagcgtc
    15481 atgatcgtgc tatttcgcta cagaataagg gaaaatggcg gttttacttt gatatacatc
    15541 atcagggagg catagcgtac atcgtccttt agaggtagaa gaaaatagtt tgtatcctca
    15601 atattggcag gttaattgct gtttccccgc aatttgcgct actgtagcgc ggataatctg
    15661 actccaggaa actatatgcg caatcgtacg ctggctgatc ttgatcgtgt cgttgctctc
    15721 ggcggagggc atggactggg acgcgttctc tcatcacttt cgtctttggg ttctcgttta
    15781 acgggtatcg tcaccaccac cgataatggt ggctcgacgg ggcgtattcg ccgctcagaa
    15841 gggggcattg cctggggcga tatgcgcaac tgcctcaacc agctgataac tgaacccagc
    15901 gtcgcttctg caatgtttga atatcgtttc ggcggtaatg gcgaactttc cgggcacaac
    15961 cttggaaacc tgatgttgaa ggcactggat caccttagcg tgcggcctct tgaagcgatt
    16021 aatctgattc gcaatctgct gaaagtggac gcacatttga ttccgatgtc ggagcatccc
    16081 gttgatctga tggcgattga cgatcagggg catgaagttt acggcgaggt caatatcgac
    16141 cagttaacta cgccgattca agagttattg ttaacgccta atgtacccgc aacgcgtgag
    16201 gcggttcacg ctatcaatga agcggatctc atcattattg ggcctggcag tttttatacc
    16261 agcctgatgc ccattctgct gctgaaggaa atcgcgcagg cattacgccg cacgccagcg
    16321 ccgatggtct atatcggcaa tctggggcgt gagttgagtt tacctgcggc caatttgaag
    16381 ctggaaagca agctggcaat tatggagcag tatgttggta aaaaggtcat tgatgcggtc
    16441 atcgtcgggc caaaagtgga tgtctcggcg gtgaaagagc ggattgtgat tcaggaggtg
    16501 ctggaggcca gcgatattcc gtatcgtcat gaccgccagt tgttacatag cgcactggaa
    16561 aaggcgttac aggctttagg ttaacggaaa tccgaaggaa aactccggct tcctattgaa
    16621 gacaaagtgc gcgttgttta tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc
    16681 gcgctaattc aatgtattgc agaatcatgt aggcctgata agcgtagcac atcaggcaat
    16741 tttgcttttg tcatctgtct tcttcgttat cctgtcacga agccgcgata aacaattcac
    16801 gcagctgatg caactggtca cgaatttgtg ccgcttcttc gaactccaga ttctgcgcgt
    16861 gttgcatcat taacccttcc agctcatgga ttttctgttg caacgcttta ggcgacatat
    16921 ccatcggcac attatccggc tcaacaatcg gtcgcgattt tcctctgccc ttcgctttgg
    16981 ttttagcaat gttctgcccc aacgccagga tatcgaccac tttcttgttc aagccttgcg
    17041 gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc gcggcgacgt tcggtttcgc
    17101 caatcgcttt cgccattgat ggggtgatct tatcgccgta gagaatcgct ttaccgttaa
    17161 cgttacgtgc cgcacgacca atggtctgga tcaacgaacg ttcggaacgc aggaagcctt
    17221 ctttgtcagc gtcgaggatc gccaccagcg aaacctccgg catatccaga ccttcgcgca
    17281 gtaagttgat ccccaccagc acgtcgaact cacccagacg caagtcgcgg ataatctcca
    17341 tacgttcgac ggtgtcgata tccgagtgaa gataacgcac gcgctcgcca tgttcttcga
    17401 gatattcggt gagatcttcc gccatccgct tggtcagtgt ggtgaccagt acgcgttcgt
    17461 taattgccgc tcgctgacga atctccgaaa gaagatcatc aacctgtgtc gccactggcc
    17521 gcacttcgat aatcggatca agcaatccgg ttggacgcac cacctgatcc accacatcgc
    17581 cgccggattt ttccagctca taattacctg gcgtcgccga aacatagatg gtttgcggcg
    17641 ctaatgcttc gaactcttca aatttcagcg gacggttatc cagcgctgat ggcaggcgga
    17701 agccgtactc caccagtgtc tctttacgcg cccggtcacc gcgatacatg ccgccaattt
    17761 gtggaatggt gacgtgagat tcatcgacca ccagcagccc atcggcaggc aggtaatcaa
    17821 acagcgtcgg cggtggctca cccggtccac gaccagagag gaagcgcgag tagttttcaa
    17881 tccccgaaca gtagcccagc tcgttcatca tctccagatc aaactgggta cgctgggtca
    17941 gccgctgctc ttccagcagt ttgttgtttt ccaacagcac tttgcgtctg gcggccagct
    18001 cttctttgat ctcctccatc gcctgtacga tgcgctcgcg cggtgtgacg tagtgcgttt
    18061 tcgggtagat ggtaaaacgt ggaatagtgg aaacaatctg cccggtcagc gggtcaaata
    18121 acgacaatcg ttccacttcc tcgtcaaaca gttccacgcg aagtgcaatg tcatccgatt
    18181 ctgccgggaa tatatcaatc acctcgccac gaacgcggaa agtaccgcgc tggaatgcct
    18241 gatcattacg agcgtattgc agctccgcca gtcggcgcag aatcgcgcgc tgatcgataa
    18301 tcataccgac cgtgagatgg agcatcatct tgagatataa atcaggatcg cccagaccat
    18361 aaatcgcgga aacagacgcg accacaacca catcacgccg ctccagcatc gctttggtgg
    18421 cggacaaacg catctgttcg atatgttcgt ttaccgaggc atctttctca atgaaggtgt
    18481 cggaactcgg tacataggct tctggctgat agtagtcgta gtaggagacg aaatattcca
    18541 ccgcgttttc cgggaagaac tctttcattt cgccatacag ctgggccgcc agcgttttgt
    18601 tgggcgcaag taccatggta gggcgctgaa ggtcagcaat gacattggca atggtgaagg
    18661 ttttcccgga accagtcaca ccaagtaacg tctggtgcgc caggccatct tccagcccct
    18721 cttcgagacg tcgaatcgcc tctggctgat cgccagaagg tttaaaagcg gaattcagtt
    18781 tgaacggttt actcatgagt cgctacctga aggagttggg cgggcaggta tgtaatttta
    18841 ctcgtcgtac ttaattatgc caacaaatta tactggataa aaaaacagtt catcaccata
    18901 atatttctgt tacagcgtaa actccgctgt caatcatgag caaaatttac tctgtggcga
    18961 gataaaactc ggaccttacc gggttatccc caaagcaacg gcttttttaa catttgtcaa
    19021 gatgagtgat gtcagtttta tggcaagaga tgcctgttca gtgactgcca ttgcaattat
    19081 ctaaccagtt aaaaaataaa agatatttct ttgagccgtc tttaacgcca attcgcgtgc
    19141 aagccgcgta ttctctcgct tgcctcgtgt tttctaactc taatacacat ggttatccac
    19201 aggaatagtg gataactgtc tgcagcccct atcccccgcc gcctgggcaa ttcccacatt
    19261 cgccgcatga tgcggaagta aaattttttt catgattatt ttagaattta attggttaaa
    19321 ttgcagtcaa tcgaagacgc gatctcgctc gcaatttaac caaatacagg atggctacaa
    19381 caaggcaagg tttatgtact ttccggttgc cgcattttct ggattttctg caagccaggg
    19441 gatctctccc agcagcggcg cgggaatcat gcgggtgagc gtggtcatat attcagcgtg
    19501 acgttttccc ggaggcgtga catcattcgc cacccaaccc gccagcgtca gtccggcgtg
    19561 ttgtattgcc tgtgcagtca acatcgcgtg attaatacag ccgagtttca cgccaaccac
    19621 cagtattacc ggcagttgtt cctgtgttac ccaatccgca aaagtgaaag tgtcggaaag
    19681 gggcgtaaac cagccgccag cgccttccac taacacccag tcagcctgtt gttcaagcgc
    19741 gcgtaatccg gagctcatta ccgatgattc tatcggcctg ccctcttgcg cgctgatgat
    19801 gtgcggtgag gtgggttctg cgaaggtgta aggatttacc gttgcgtaat ccagcagaag
    19861 gctgctgttg cgctgtaacg ccaacgcgtc actattacgt aaaccctccg gggtcttttc
    19921 gctgccagag gcgaccggtt tataacctgc cgtccgataa cctactgcct ttgcggcttg
    19981 taaaagtgca caactggcga cagttttccc cacttcggta tccgttccgg tgacaaaata
    20041 acgtttactc acgaacaatc actcccaaaa aaagatgata cgtcagagga tatcgcccct
    20101 gttgttgcgg ccaggccagt tgcaatcgct gcaactgtga acgcgttaat attcgctggt
    20161 cgcgcccttc atgaagatgc gtggcaccga tgcctttcag cgaacgcatg gcactgagcg
    20221 catcatcaaa ccatagtgtg atgggctgaa tacgatgttg ataatgcacg ccgttcagcg
    20281 actgttcgat ttcatctggc ggtaaaaagc gattagcatg cgggcgctcg tccaccgcct
    20341 gccacgcctg atgcagttcg ggtaacgatc cctgcaccag cgtggtaaac gcgaccacgc
    20401 ctccggggcg caccacccga tacagctcgc ggagtgccgt tgataaatta ccgcaccact
    20461 gcaccgcgag attgctccat gcaagatcga acgtcgcagt cgctaacggc agggattcga
    20521 tatctcccgc cagataatgg tctgcagcat ctttctggcg ggcttgcgca agcattggcg
    20581 gcgagagatc taacgccgtc acctgcgcgt gacgctctcg ccagtggcgg ctcatccagc
    20641 caggcccaca ccccgcgtcc agtacgtggg tgtatttacg ctgtgggagc attaccagta
    20701 agacgtcagc actctggcgc tgtaactctg cgtgttgctc atagtgcgcg gctgcccgac
    20761 caaatgccgc tgcaatggct tgtttattaa ccgttgccat gcagcacctc cagcagacgg
    20821 tcgatatcct gcatttcatg cgcggcggtt agcgttaagc gcagtcgcgc agtaccagcg
    20881 ggtacggttg gcgggcgaat cgccgtgacc cagcagcctt gctggcgcag tttttctgcc
    20941 agttgtaacg cacggctgtt atcaccgacg atcaatggct gaatggcgct gcatgaatca
    21001 gccagcgtaa acggcaaatc ctgtactccg gcacgaaaac gcgtaatgag tgccgccagt
    21061 ttttcgcgcc gtgcatcacc ctcatcactg cgaatgaccg ccagcgacgc acgtaatgcc
    21121 tgcgcctgag cgggcggcat actggtgctg tagataaggt ggcgggcgaa ttgcagcaga
    21181 taatccgcca ccgtactgga gcaaagcacc gctgccccac tgacgccaaa tcctttgcca
    21241 aaagtcacca ccagcaattc tggttttacc ttttgcagcc agcagctgcc acgcccctga
    21301 tccccaataa cccccgtgcc gtgggcatca tcgaccatca accagccatt gtgctgttgc
    21361 gttacctgct ggatttcccc cagtggcgca ctatcgccgt ccatgctgaa cacgccttct
    21421 gtcaccacca gttgctgccc cggacagggg gaagcaagca gtcgcgccag atgagtgaca
    21481 tcgttatgaa aaaaacggcg aagctgcgac gggcttaaac tggcagcctc cagcaatgag
    21541 gcatggctaa gccggtcagc gacaatgcgg tcctcttttg ccatcatcgc ggcaatgacc
    21601 gcctgattag cggcaaaacc agagataaat agcagtgccc gcgaatagcc cagccactcg
    21661 gccagttctt cttccagcgc ctgatgcgcc acgctataac cgctgacgtg accggaaccg
    21721 ccgctaccga cgccaaattg ctccgcactc tgcttccagg cacggataat ttgcggatga
    21781 tggcttaaac ctaaataatc gttactggaa aagttcagat actggcgatc gtccgccacc
    21841 agccagcgtc cggctccttg cgccactgga taacggcgac gcagggcatc ggcagcacgc
    21901 cgcgcatcga gcgccgcgtt gattttctcc tgccagctca tagtgctgcc gcgttgtaat
    21961 attcgtcagt gtccggggtc atcagtgcct gttccagacg ctgctgttgt tcgttatcgc
    22021 ccgccagcac ggcagtttgc tgcggattta gccccagttt gcggaacagt tgcaggtctt
    22081 tatcttcttc cggattcggc gtggttagca gtttgcaacc gtagaaaatc gagtttgcgc
    22141 cagccataaa gcacattgcc tgagtctgtt cattcatctg ctcgcgtccg gcagaaaggc
    22201 gcacgtaaga ggtcggcatc atgatccgcg ctacggcaat ggtgcgaata aaatcaaagg
    22261 catcgacatc atcgttatct gccagtggag tacctttcac cttcaccagc atgttgattg
    22321 gcacgctttc cggcggcgtc ggcaggtttg ccagttgcag taataatccg gcgcgatctt
    22381 ttaccgtttc gcctaaaccc acaatgccac ctgagcagac tttgatcccg gcatcgcgca
    22441 ctttttccag cgtatcaagg cgttcctgat aagtacgtgt ggtgatgata ttgccgtaaa
    22501 actccggcga ggtgtcgagg ttatggttgt agtaatccag cccggcattc gcaaggcgct
    22561 gcgcctgaga ttcactcaac gtacccagcg tcatacacgc ctccagcccc atcgctttta
    22621 ctccctgcac catttgttcc aggtatggca tatcgcgttc gtggggattc ttccacgccg
    22681 cgcccataca gaagcgcgtc gatcctgccg ctttcgcttt gcgcgccgac tccagcacct
    22741 gttcaacttc catcaaccgc tcggcttcca gcccggtttt gtagcgcgag ctttgcgggc
    22801 agtatttgca atcttccgga caagctccgg tcttaatcga cagcagagtg ctgacctgca
    22861 cctgacgagg atcgaaatgc tggcgatgga cctgctgcgc ttcaaacagc agatccagca
    22921 acggtttatc aaataattct gtgacttgcg acaatgtcca gcgtgggcgg tgagccatgg
    22981 ggcttctcca aaacgtgttt tttgttgtta attcggtgta gactcgtaaa cctaaatctt
    23041 ttcaatttgg tttacaagtc gattatgaca acggacgatc ttgcctttga ccaacgccat
    23101 atctggcacc catacacatc catgacctcc ccgctaccgg tttatccggt ggcgagcgcc
    23161 gaaggttgcg agctaatttt gtctgacggc agacgcctgg ttgacggtat gtcgtcctgg
    23221 tgggcggcga tccacggtta caatcacccg cagcttaatg cggcgatgaa gtcgcaaatt
    23281 gatgccatgt cgcatgtgat gtttggcggt atcacccatg cgccagccat tgagctgtgc
    23341 cgcaaactgg tggcgatgac gccgcaaccg ctggaatgcg tttttctcgc ggactccggc
    23401 tccgtagcgg tggaagtggc gatgaaaatg gcgttgcagt actggcaggc caaaggcgaa
    23461 gcgcgccagc gttttctgac cttccgcaat ggttatcatg gcgatacctt tggcgcgatg
    23521 tcggtgtgcg atccggacaa ctcaatgcac agcctgtgga aaggctatct gccagaaaac
    23581 ttgtttgctc ccgccccgca aagccgcatg gatggtgaat gggatgagcg cgatatggtg
    23641 ggctttgccc gcctgatggc ggcgcatcgt catgaaatcg cggcggtgat cattgaaccg
    23701 attgtccagg gcgcaggcgg gatgcgcatg taccatccgg aatggttaaa acgaatccgc
    23761 aaaatgtgcg atcgcgaagg tatcttgctg attgccgacg agatcgccac cggatttggt
    23821 cgtaccggca aactgtttgc ctgtgaacat gcagaaatcg cgccggacat tttgtgcctc
    23881 ggtaaagcct taaccggcgg cacaatgacc ctttccgcca cactcaccac gcgcgaggtt
    23941 gcagaaacca tcagtaacgg cgaagccggc tgctttatgc acgggccaac ttttatgggc
    24001 aatccgctgg cctgtgcggc tgcaaacgcc agcctggcga ttctcgaatc tggcgactgg
    24061 cagcagcagg tggcggctat tgaagtacag ctgcgcgagc aactggcacc agcccgtgat
    24121 gccgaaatgg ttgccgatgt gcgcgtactg ggggcaatcg gtgtggtcga aaccactcgt
    24181 ccggtgaata tggcggcgct gcaaaaattc tttgtcgaac agggtgtctg gatccggcct
    24241 tttggcaaac tgatttacct gatgccgccc tatattattc tcccgcaaca gttgcagcgt
    24301 ctgaccgcag cggttaaccg cgcggtacag gatgaaacat ttttttgcca ataacgggaa
    24361 gtcagcgtga gggtttctgg ctacactttc tgcaaactag aaaggagggt tcatgaaact
    24421 catcagtaac gatctgcgcg atggcgataa gttgccgcat cgtcatgtct ttaacggcat
    24481 gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc ctgcgggaac
    24541 gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca accggctccg gctggtggca
    24601 ctgggtagtt gttaatttac ccgctgatac ccgcgtatta ccgcaagggt ttggctctgg
    24661 tctggtagca atgccagacg gcgttttgca gacgcgtacc gattttggta aaaccggata
    24721 cgatggcgca gcgccgccga aaggcgaaac ccatcgctac atttttaccg ttcacgcgct
    24781 ggatgtagag cgtattgatg tcgatgaagg agccagcggc gcgatggtcg ggtttaacgt
    24841 tcatttccac tctctggcaa gcgcatcgat taccgcgatg tttagttaat cactctgcca
    24901 gatggcgcaa tgctatctgg tatcacttaa aggtattaaa aacaactttt tgtcttttta
    24961 ccttcccgtt tcgctcaagt tagtataaaa aagcaggctt caacggattc atttttctat
    25021 ttcatagccc ggagcaacct gtgaacacat tttcagtttc ccgtctggcg ctggcattgg
    25081 cttttggcgt gacgctgacc gcctgtagct caacaccacc cgatcaacgt ccttctgatc
    25141 aaactgcgcc tggtacctct tctcgcccga ttctgtcggc aaaagaagcg cagaatttcg
    25201 atgctcaaca ctattttgca tccctgacac caggtgctgc agcgtggaat ccgtccccga
    25261 taaccctgcc tgcgcaacct gactttgttg tcggcccggc gggtactcaa ggtgtaacgc
    25321 ataccacgat tcaggcggcg gtagatgcgg caattatcaa gcgcaccaac aagcgccagt
    25381 atattgccgt gatgcctggt gagtatcagg gaacggtgta tgtccctgct gctccaggtg
    25441 gaattactct gtacggtaca ggtgaaaaac cgattgatgt gaagattggg ctttcccttg
    25501 atgggggcat gagccctgcc gactggcgtc acgacgtcaa cccgcgcggc aaatatatgc
    25561 caggtaaacc agcgtggtat atgtacgata gctgccagag caaacgcagc gacagtatcg
    25621 gtgttctgtg ttctgcggtc ttctggtcac aaaacaatgg cctgcaactg caaaacctga
    25681 ccatcgaaaa cacgctgggt gatagcgtag atgcgggtaa ccatccggcg gtggcactgc
    25741 gtactgacgg tgaccaggta cagattaaca acgttaacat tctcggtcgt cagaacacct
    25801 tctttgtcac caacagcggt gtgcagaacc gtctggaaac gaatcgtcag ccgcgtacgc
    25861 tggtgaccaa cagctacatt gaaggggatg tggatatcgt ttctggtcgc ggcgcagtgg
    25921 tgttcgataa caccgaattc cgcgtggtga actcacgtac tcagcaagaa gcgtatgtgt
    25981 ttgcaccggc tacgctgtcc aacatctatt acggtttcct cgccgtaaac agccgtttca
    26041 atgcttccgg tgatggcgtg gcgcaactgg gccgctcgct ggatgttgat gccaatacca
    26101 acggtcaggt ggtgatccgt gatagcgcca tcaacgaagg ttttaacacg gctaaaccgt
    26161 ggggcgatgc ggtgatctct aatcgtccgt ttgcgggtaa caccggcagc gttgacgata
    26221 gcgacgaaat acagcgcaat ctgaatgaca ctaactacaa ccgcatgtgg gaatacaata
    26281 accgcggcgt gggtagcaaa gtggttgcag aggcgaagaa gtaagagcta ttaactattt
    26341 gccggatgcg gcgtaaacgc cttatccggc ctacggttcg atgcgatttg taggtcggat
    26401 aagatgcgcc agcatcgcat ccgacaatac gtgccgaatg cgacctacat tcacatggcg
    26461 ctttttacat ctgacggttt ttattgaagt taatcaaact acccgccttg ataatctcgc
    26521 gctcttcagc cgtcaggctt tccatgtaaa gcgtaatttc cgttaccggc gcatcttcat
    26581 ggatcacata acctttaaac gtcgtacccg gattatccag cgccgcttta atgccaggga
    26641 tgtaaatgta atcccccact tcaaaggttg gtacttccgc catttgcagc ggtaacatcc
    26701 cccagttgat gacgttagaa cgatagcgtt tagtcgcgta ctcctcggca atattcgcca
    26761 gaccgccaat cacacgctgg cagctcgccg cctgttcacg cgcagaacca tcgcctggtt
    26821 tcaccgcata gaccatactg ccaatttcag tttgcagcgg atcaatatgc tcctgaccag
    26881 caatctgctt aatgcgcgca aacacctctg tcagctcgct gacattcccc gccagacgct
    26941 gattttccag ctcagcagtc gctttacttc tgccaacata accgggatcg cggcgagaca
    27001 gggtaaactc cgccagacca atcggatttg aacgataaga agaggtttca ccggaaggga
    27061 tcagttcgtc ggtggtggtc acttcgtcga ggatcttcga gcacactttg aggacgatat
    27121 tgtcagtcag cgcacctaat tccggccagt ctttaatgtt cggtccgtaa atcagcggtt
    27181 gctgagttgc ccctttcaca aagccctgat aaacacggtt tttatacggc gttacatcga
    27241 aggcgtactc cggcacgttg tcccagcaat cgagttcgct ggcagaagtt aaatagccac
    27301 cgtttgccgc agtcgcagcg atagaacgag cgtccatcaa cgccaccgct gacatctgcc
    27361 cattggctgg cttagagcct tcgcggttcg ggaagttacg cgtggtgtgg cgaatactta
    27421 aaccgttgtt gattggcgta tcgcccgcgc caaagcatgg gccgcagaac gcggttctga
    27481 tgattgcgcc tgcgccaatc aaatctgcta ccacaccttt tttggcgaga tccataaaca
    27541 ccggctgtga tgacgggtaa accgccagcg agaaggtgtc attgccacag gattgaccgc
    27601 gcagtgcatt cgccgccgcg atgacgtttt cgtagttacc gccagaacag cccgcgataa
    27661 tcccctgctg cactttcagg cgaccatttt ccactttgtc cagcagcgag agtttggctt
    27721 taccgtgcgc cacgcgttcg gactcaattt caatctcacg cagaatgtcg gtcaggttct
    27781 ggttcagtgt gtcgatttca tacacgttgc tcgggtggaa cggcagcgca atcattggtt
    27841 tgatggcgct taaatcaacg ctgatgcagc catcgtagta cgccatcggt tgagggttaa
    27901 gctggcagta atcctggccg cgaccgtgca gcgccagcca gttatggact tcttcatcgg
    27961 tttgccagac agaacttaaa caggtcgttt cagtggtcat aacgtcaacg ctgttacgga
    28021 aatcggtaga gagcgcagca acaccgggac ctacgaattc catcactttg tttttgacgt
    28081 agccgttttt gaacacggca ccgatgatag ccaacgccac atcctgcggc cccacatacg
    28141 gcgctggttt tccggtcaga tgcaccgcaa caactcccgg atagtcgata tcccaggtgt
    28201 cattaagcaa ttgttttacc aactcgccgc cgccctcacc gactgccatt gtccctaatg
    28261 caccgtaacg ggtgtgactg tctgacccga ggatcatttt gccgccacct gccatcatct
    28321 cacgcatata ttgatggatg accgcaatat gcggaggcac aaaaataccg ccataacgct
    28381 gagccgccga taaaccaaaa acatggtcat caccgttaat ggtgccgccg acggcgcaaa
    28441 gtgagttatg gcagttggtc agcacatacg gcagcgggaa acgttccata ccggacgctt
    28501 tagcggtctg tacaatacca acaaaggtaa tatcgtgaga ggctaatgaa tcaaacttaa
    28561 ttttaagttt atccatattt ccggacgtat tatgagaaga gagaatagac caggcaatag
    28621 tgcctttttt ggcttcttct tttttaattt cgccggtgaa atgttcttcg gcaattattt
    28681 cgttattact ggcgagaaac acgccttttt cagataactt gatcattaaa tgctccagat
    28741 atctgcgcac cgtgaagtgc gcagaaagat aataaatgga tatactctaa ataattcgag
    28801 ttgcaggaag gcgacaagcg agtgaatccc caggagctta cataagtaag tgactggggt
    28861 gaacgaacgc agactcagca catgcaactt gaagtatgac gagtatattt acagcatgtt
    28921 ccagccgatc agcatattcc accaccacac tccgagggtg atatgcacca ggaaggttaa
    28981 tatcgtcagt accgcaccga ccaaccacca ggatttaata tcgttataac ccacgccaaa
    29041 gatgaccgga cctgccgcgc cgccatagtg agtaaccatg ccgccatagg agttggagaa
    29101 caacagtgcc agcgcggtta acattaacgg tgcgccggag acgttcgcca gcatggcaaa
    29161 taccggtaac atagcaacga tataggcact accggaagcg aagaaataac gcacgatgat
    29221 gctgaggaaa ataataacga agaaagcaac gtttccgtga ccatcaaatg ccaggttatt
    29281 tttaaagact tcagctaacc attcgaagaa tttaactttc gataataagg aacttaagcc
    29341 gataataccg ccgtaccaga ttaaggtatt ccagccgcct ttatttttaa ccacgtcttc
    29401 ccaggtaacg atacccagca gcagcatggt cgccataaca acaatagcca ctgaagattc
    29461 atcaaccccc agagacttac tgaaaatcca gcccagcagc gccagcacaa agacaccgag
    29521 cagcattttt tcgcggattt tcatcggtcc cagttcggca aggcccgctt tagcgatggt
    29581 tttgttatcc accttcttaa tttctggtgg atacatggtg taaatcacca gcggggtgac
    29641 cagcagcata atgatgcccg gcaatccggc ggccagcgcc catccacccc agctaatttg
    29701 caggtgcaga atgtcgttga tcattttcag cgccagaatg ttccccgcca ttgcggtaaa
    29761 gaacatatag ctggtggttt tggtgaccat gtaaatggac atcatcaggt aatgtccgac
    29821 acgacgcgga cttttttccg gttcggaccc caaagccacc gccacgctgt tgatgatcgg
    29881 taacacaatg ccgcccgcac gcgcggtgtt agacggtgtt gccggagcca ataccagatc
    29941 gaggaatacc gtaacgtaac ccagacccag cgtggtgtta ccgattttac caatcagcag
    30001 ataggcaata cgtttaccta aaccggtggt cacaaatgcg gcgcttaagg taaacgccga
    30061 gaacaccagc caggtagtac ctgaagagta accgcttaat acggcggtgg tcttaaacgc
    30121 accgtcggat aagttaccga ccaccaccat ggaggcagca acggcaatta acagtacgac
    30181 aggttccggg aaaggcttga taaccagccc cacaatggcc gccaggtaaa taccaaaaag
    30241 cacccacgcc agttcgctta atcctgccgg agctggcata aagccaataa tacatgggat
    30301 cgctaatatc agaattagct tccataacga tttcttattc atttaaaatc ctgttctttc
    30361 tgtatgactc atacagaagt cctttattga ttgtccttat cctgacaacg aatcttttca
    30421 gggaagataa acttcaccgg aaaatatttt tctggtcgtc cgaataacag atgcgcgtaa
    30481 cgtcgtggca tcctgacctt cattacttaa atgaacgtcg agcgcaccac tggggtgttc
    30541 aatattgata ttgccatatc ctacagaagg gatgatttgt cgggtgacgg tgccttccaa
    30601 tgcacaacta ctggaaatag caatagcacc ggttatcgcc aacgcgcgat ggcaagaatg
    30661 cggcataaaa taacgcacat taattgcccc gcctttctgc gctggagaaa taagcacagg
    30721 tttagggata accatattac tgacatcacc taagcccatt gctttacccg cttgtagacg
    30781 gatagattca atgcgggcta ataatgcttt gtcggcatcc agttccgccg gtaattcata
    30841 gcctgttttt cccagatatt cagccggaat aatgacgact ggcatcgcca tatcgataca
    30901 ggtcaccggg acatcgtcaa aataatcaat ctgattatca gtcgggaaaa cttttccggt
    30961 tttggttcca gcggcattca ggaaagtgag cgcaaccggt gcggcagtac ccggtacgcc
    31021 gtcaattctg gcactaccct cgtactcgac aacaccattt ggcgtttgca catcagcttc
    31081 gataaacgta cccgtattga cgttgcggat acgtacgcgg gtaactggcg aagtcgctgc
    31141 aatcaaacca ttttcaatgg cgaatgcccc aacgccagac agcatattgc cacagttagg
    31201 cgtggtatcg acacgttgct catggacgat aacttgagca aacagataat caacatcagc
    31261 acgcggatcg ctggaacggc ttataatggc gactttactg gtcagtggat taccgccgcc
    31321 aataccgtca atttccagat cgttaccgga acccataatt gccatcaata ttttatcgcg
    31381 ctgcgtttga tcttcgggta aatgttccgc taacaggaac gcgcccctcg aggttccacc
    31441 tcgcatcatc acgcagggta tttttttcat aataatgccc tcaatttaaa aacataactt
    31501 attgcgatat ggttacatta agggcaaagc atctctaatg actttagctc aacgattaat
    31561 aacatttttt cattaattta agttggctaa atgcagttaa aatttttaac ggccagccac
    31621 ccaaaatcga cgcttccatt ttccggagta ataatgaagc atgaattatc aagtatgaag
    31681 gcatttgtca tactggcaga gtccagttca tttaacaatg ccgctaaatt actcaacatt
    31741 acgcaacctg cattaacgcg aagaataaaa aagatggagg aagatttaca tattcaacta
    31801 tttgagcgta caactcgcaa ggttacgtta acaaaagcag gaaaaaggtt gctcccggag
    31861 gcgcgggaat taataaagaa attcgatgaa acgcttttta atattcgtga tatgaatgct
    31921 tatcatcgtg gtatggtgac gttagcgtgt attccgaccg cagtatttta ttttttaccg
    31981 ctggcaatcg gtaaatttaa tgagctgtat cccaatatta aagtgcggat tctggaacaa
    32041 ggcacgaata attgcatgga atcggtgcta tgtaacgaat cagactttgg cataaacatg
    32101 aacaacgtca caaattcatc cattgatttt agcccactgg tcaacgagcc gttcgtgctg
    32161 gcctgtcggc gtgaccatcc attggccaaa aagcagcttg tagaatggca ggagttagtg
    32221 ggttacaaaa tgattggcgt acgctcctcc agcggcaacc gactgttgat agaacaacag
    32281 ctagccgaca agccctggaa gctggactgg ttttacgaag tgcgtcatct ttcgacgtca
    32341 ttaggactgg tggaggcagg actggggatt tcagcgcttc ctggtctggc aatgccccat
    32401 gcgccctatt cttcgattat tggtatccca ctggtggaac ccgttatacg tcggacattg
    32461 gggattattc gccgcaaaga tgccgtgctt tctccggcag ctgaacgctt ttttgccttg
    32521 ctgattaatt tgtggactga cgataaagac aatttgtgga ccaatatcgt cgaacgccag
    32581 agacatgcgt tacaggagat aggttaatac accggcgcag gagagactgc gccgggtaaa
    32641 tcagcggtta gtgtgcgtta accaccaccc acattggccc ctgcccgacc gcgtagcggc
    32701 ctttttcatg cagtagcccc tgctcaccaa caatttcgta taccgagata tggtgagatt
    32761 tttgcccggc ggcaatcaga tacttaccac ggtgatcgat attaaagccg cgcggctggg
    32821 tttcagttgg ctggaagccc tctttactca acacgctgcc atcttccgaa acgctgaaga
    32881 cagtaatcag gctggcggta cggtcacagg catataagtg acgaccatcc ggggtaatat
    32941 gaatatcagc cgcccaacgg gtgtcggaga agttttccgg catcatatcc agcgtctgga
    33001 cacattcgat attgccgtgc ggatctttca gttcccagac atccactgag ctgtttaact
    33061 cattgacgca atacgcatat tgttcgtttg gatggaatac catatgacgc gggccggccc
    33121 cttcaacggt agtcacttcc gcagggtcct gcgccaccag atgaccatca tcgctgaccg
    33181 taaacaggca aatgcgatcc tgctttaatg ccggaaccca cagcgtacgg ttgtccggtg
    33241 agatattggc ggaatggcaa ccgtccagcc cctcgaccac atcgacgacg ccaactggca
    33301 ggccatcttc cagacgcgtt acgctcacgt tacccgcatt gtaagaacct acaaagacaa
    33361 actgcccctg gtgatcggtg gaaatatgcg tcggactacc cggcagcgca gactctgcgg
    33421 caaaggtcag tgcgccatcg tccggggcga tacgatacgc caggacgcga aactcagggc
    33481 gaacaccaac atagagataa cgtttgtccg ggctgaccac catcggctgc acctgccccg
    33541 gcacatcgac aacctgtgtc agcgtcagtg cgccttcatg attcaggttc cagacgtgaa
    33601 tttgctggct ctcagggctg gcgatataaa ctgtttgctt catgaatgct cctttgcatt
    33661 tagcaactga ctgtaacagc taaaattagt cgcttttggc ggtgaatgct gaattaaaac
    33721 gcagaatttt gtccgatagt ggtatcggtg taccattcgc gcagaagaaa attcttaatc
    33781 tggaatacgc catgaccaca cgcgtgattg ctctcgactt agacggcacc ttattgaccc
    33841 cgaaaaagac cctgctacct tcatcgatag aagccctggc ccgcgctcgc gaagcaggtt
    33901 atcaattaat catcgtcaca ggtcgccatc acgtcgctat tcatcctttt tatcaggcgc
    33961 tggcgctgga tacacctgct atttgctgta atggcaccta tttgtatgat tatcatgcaa
    34021 aaactgtgct tgaagccgat ccaatgcctg ttaacaaagc cctgcaactc attgagatgc
    34081 tgaatgaaca ccacattcac ggtctgatgt atgtcgatga cgcgatggtt tatgagcacc
    34141 cgaccgggca tgtcattcgc acgtctaact gggcgcaaac cctgccgccg gaacagcgtc
    34201 cgactttcac acaagtcgct tctctggctg aaacggcgca acaagttaac gccgtatgga
    34261 agttcgccct cacgcacgat gacctgccgc aattgcagca ttttggtaag catgtcgaac
    34321 atgaactggg actggagtgt gaatggtcct ggcacgatca ggttgatatt gcacgcggcg
    34381 gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc gcaaggctgg tcgatggaaa
    34441 acgtcgtggc gttcggcgat aactttaatg atatcagtat gctggaggcc gctggtacag
    34501 gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg cgccaacatt gtgattggtg
    34561 ataacaccac cgacagcatt gcccagttca tttatagcca cctgatttaa tcaggcggtt
    34621 atcgacacac ttttaatttg cgcgtacagc cacaggccag gtttgatcgc cagttcatcc
    34681 ctggcccacg ggctgatacg cgcccacagc gttttaccgc cgacttccag ttccacttcc
    34741 acctggccgt tgtcgtcata actattaaca acttttgccc gcaatacgtt acgaatgctg
    34801 gtttgctgcg gcggttgtaa aaccagagaa acatcggaag cctgaatgcg gatacgtagc
    34861 gcagcttgca gcggttcgtc cagcttattg acccacaaat gctgatcgcc cagcgccagc
    34921 gcagtcatcg cgtaatgcgg atgatgctca agtaccgtca ctttcagaat gctactttgt
    34981 tgctctttcg gcagccacgg attcatcacg ctactacccc agacttcttc cagcgcgcca
    35041 aaggctttca cctgaccgtt ttccagtacc atcactctgt ctgccagatg gaggatctca
    35101 tccagcgaat ggctgacata caacatcggg atgttgattt cccgcgtcag acgttgcaga
    35161 taaggcaaca gttcgcgttt acgcggaata tccagtgacg ccagcggttc atccagcaac
    35221 agcaattctg gtgccgtcag caaagcccgc ccaatcgcta cgcgctgttt ttcgcctccg
    35281 gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc ctaaaagcgc caccagctta
    35341 tcgaactgat cgaccatgct tttcgccatg ccgtagcgca gattgccacg cactttgtaa
    35401 tgcggaaaca gccgcgcatc ctgaaaaaca tagccaacgc gacgcttttc tggcgtcagg
    35461 cagatacctt tttcggcatc atttagtacc cgaccattga ggacaatcct ccctttttgc
    35521 gggcgcgtca gtccactgat ggcgttaatc agcgaagttt ttccggcacc ggagacgcca
    35581 aagatagcag tgatgccatt ggcgggcagc gtttcattaa tagtcaggca atggttgccc
    35641 aacgtctggg aaaaattcag ttccagcatg attagcgccc cgcccgttca cggctgattc
    35701 tggccagcca ttctgaaacc aacagggaga tcatcgccag cgcaatagaa ataatgcaca
    35761 gtctcgctgc gccactttcc ccgccggggg tctgaatcag ggtatacatg gcagaaggaa
    35821 tggttcgcgt ttcaccagga atgttcgaca caaaggtgat ggttgcgcca aattcaccga
    35881 gagaacgagc aaaagccagt accgtaccaa caataattcc cggtaaggtc agcggtaacg
    35941 tgatagtaaa gaaaacgcgc cagcgcccgg cccccagtgt tcttgcggcc tgttccagtt
    36001 tgacatcaac cccttccagc gccagacgaa ttgcccgcac catcagcgga aacgacatga
    36061 cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa ggttatacca aaccagtcat
    36121 acagacgttc accgataaat ccgcgccgcc ccatcgaaac taataataag taaccgacga
    36181 ccacgggcgg taacaccagc ggtagatgca gtacgctgtc gagcagagat ttgcccggaa
    36241 acgtgcaacg caccagtaac caggcaaaaa agatcccaaa cggcaggcta aacagcacag
    36301 ccagggaaga aactttcagg cttaataaaa ctgcctgcca ttctggatcg gtcagtatca
    36361 ttacttggtt gtaaatccgt aacgtttaaa gatttccgca gcctgcggtc ccttcagata
    36421 atcataaaat gctttcactg tcgcattgtt atgcccttcc acaacagcaa ccggatattc
    36481 cactttttta tgtgaatctt ccggaaaggt ggcaaccact tttacccctt tgctggcaac
    36541 tgcgtcagaa ccgtaaacaa tgcccaacgg cgcttcattg cgttcgacca acgccagcgc
    36601 gccacgaaca tcttccgccg gagccagttt cggggagagc gtatcccatg cgcccagttt
    36661 ttgcagtgct tcttttgcat aaatgccagc agggacatgt tccggatcgc caaccgcgag
    36721 gcgaccgcca ttcagcagcg aagtccagtt agttttgctg tcgatgatga aatctttctg
    36781 ctcgctggct ttcggtgcta caacgaccag gctattgccg agcagtgtct gacgcgttgc
    36841 tgtatcgatc gcttttttat caaccgcata atccatccat ttctgatcgg cagaaataaa
    36901 cagatccgca ggggcgcccg cttcaatctg acgggcgaga gtagatgacg aagcgaaaga
    36961 agaaaccaca tccacgcctt tctctttttt atactgcgta gcaatgtcct gcattgcgtt
    37021 agtcagtgat gcggcggcga acaccgtgat tttcccttca tctgccagtg cattgccagc
    37081 aacagcgaaa gagagtgccg ccccggcaaa caagttcaac catttacgag ccattggtaa
    37141 ccccttaatg taacgttatg taggcgacaa tataacgact aactcggaat tttcccagca
    37201 gttattgcta acctttgtga ggtagataag aaaaaatatc ggcaggaaaa gcaggaagtt
    37261 gagaaaaaga aaatgcccga ctaagcgggc attcagggaa tcaatgattt tgtccaggct
    37321 ggtctttttt accaacacca gaaaagatgt tgaatacttc accaagaccg taaatcagac
    37381 ccaggatgat ggccatcacg acaggtacca tgattacggc gaataccaga ctttttaata
    37441 actctaacat ggtcaactcc agatatagtc atgagactat tctaaccgct aagcacagaa
    37501 aagcactccc cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc acaataagac
    37561 ttttgccagg acattgttat gcaggccgaa atccttctca cccttaagct ccaacaaaaa
    37621 ttattcgccg acccgcgccg catttcgcta ctaaaacaca ttgcgctttc cggttccatt
    37681 agccagggag cgaaagatgc cggtatcagc tataaaagcg cttgggatgc cattaacgag
    37741 atgaatcagt taagtgagca tattctggtc gagcgcgcaa caggcggtaa aggtggcggc
    37801 ggcgcagtac tgacccgcta tggtcagcga ctgattcagc tctatgactt actggcgcaa
    37861 atccagcaaa aagcctttga tgtgttaagt gacgatgacg ccctgccgct gaacagcctg
    37921 ctggccgcga tctcacgttt ttcactgcaa accagcgccc gtaaccagtg gttcggtacc
    37981 atcaccgccc gcgatcatga tgacgttcaa cagcatgttg atgtcttact ggctgacgga
    38041 aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg cgcgtctggg gctggatgaa
    38101 ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag gcattactca ggacgaagcg
    38161 gtcgcgcaaa acgctgacaa ccaattaccg ggtattatta gtcatattga gcgcggcgca
    38221 gagcagtgcg aagtattaat ggcgctaccg gacgggcaaa cactgtgcgc cacagtgccg
    38281 gtaaatgaag cgacttctct tcagcaagga cagaatgtca cggcctactt taatgccgac
    38341 agcgtgatta tcgccacgct gtgctaagcg tgttgacaat ttgttatgaa acacgtatcc
    38401 ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa ttttgcaagg
    38461 cacgtttcgt cttagcgaca caaaaacgct gcaattgcct cagctaacgt taaacgcggg
    38521 tgatagttgg gcgtttgtcg gttcgaatgg aagcgggaaa tcggccctgg cccgcgcgct
    38581 ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc cagttttccc acatcactcg
    38641 tctctccttc gagcaattgc aaaagctcgt cagcgacgaa tggcagcgga ataacaccga
    38701 tatgctcggc cctggcgaag atgacaccgg acgcactacg gctgagatta ttcaggatga
    38761 agtaaaggat gcaccgcgct gcatgcaact ggcacagcag ttcggtatta ccgccctcct
    38821 cgaccgacgc tttaaatacc tttccactgg cgagacgcga aaaaccctgc tgtgtcaggc
    38881 gctgatgtcg gagcctgact tgttgattct tgatgagccg ttcgatggcc tggatgttgc
    38941 ctcacgtcag cagctggctg agcgactcgc ctcgttacat cagtccggta ttactctggt
    39001 actggtgctc aatcgcttcg atgagatccc ggagtttgtc cagtttgctg gcgtgctggc
    39061 ggattgcacg ttagcggaaa ctggcgctaa agaggaactg ctccaacaag cactcgtcgc
    39121 gcaactggcg catagtgaac agcttgaagg tgtgcaactg ccggagccgg atgaaccttc
    39181 agcacgtcac gccttaccca ccaacgaacc gcgcattgtg ctgaataatg gcgtggtttc
    39241 ttataatgat cgccccattc ttaataacct tagctggcag gtgaatcctg gcgagcactg
    39301 gcaaattgtc gggccaaatg gtgcgggaaa atcgacgtta ttaagcctga ttactggcga
    39361 tcatccgcaa ggttacagca acgatttgac gcttttcgga cgacgtcgtg gcagcggcga
    39421 aaccatctgg gatatcaaaa agcatatcgg ttacgtcagc agtagtttgc atctggatta
    39481 ccgggtcagt actactgtgc gtaatgtgat tctttctggc tattttgatt cgattggcat
    39541 ttatcaggcc gtttcggatc gccagcaaaa actggtgcag cagtggctgg atattctcgg
    39601 cattgataaa cgcacggctg acgctccgtt ccatagtctt tcctggggac agcagcgtct
    39661 ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg cttattctcg acgaaccatt
    39721 gcaggggctt gatccgctga atcgccagct tattcgccgt tttgttgatg tgctgattag
    39781 cgaaggtgaa acgcaattgt tgtttgtttc gcaccatgct gaagatgcgc cagcttgtat
    39841 tacccatcgt ctggagttcg tgccggacgg tggactctat cgctatgtgc tgacaaaaat
    39901 atattgagtc ggtagtgctg accttgccgg aggcggcgta aacgctttat ccggcacacg
    39961 gtcagacgca tccaggcatg aaactgcgtc tttttttcag ataaaaagcg caatcattca
    40021 caatccctct gttttataat cacttaatcg cgcataaaaa acggctaaat tcttgtgtaa
    40081 acgattccac taatttattc catgtcacac ttttcgcatc tttgttatgc tatggttatt
    40141 tcataccata agcctaatgg agcgaattat gagagttctg gtcaccggtg gtagcggtta
    40201 cattggaagt catacctgtg tgcaattact gcaaaacggt catgatgtca tcattcttga
    40261 taacctctgt aacagtaagc gcagcgtact gcctgttatc gagcgtttag gcggcaaaca
    40321 tccaacgttt gttgaaggcg atatccgtaa cgaagcgttg atgaccgaga tcctgcacga
    40381 tcacgctatc gacaccgtga tccacttcgc cgggctgaaa gccgttggcg aatcggtaca
    40441 aaaaccgctg gaatattacg acaacaatgt caacggtact ctgcgcctga ttagcgccat
    40501 gcgcgccgct aacgtcaaaa actttatttt tagctcctcc gccaccgttt atggcgatca
    40561 gcccaaaatt ccatacgttg aaagcttccc gaccggcaca ccgcaaagcc cttacggcaa
    40621 aagcaagctg atggtggaac agatcctcac cgatctgcaa aaagcccagc cggactggag
    40681 cattgccctg ctgcgctact tcaacccggt tggcgcgcat ccgtcgggcg atatgggtga
    40741 agatccgcaa ggcattccga ataacctgat gccatacatc gcccaggttg ctgtaggccg
    40801 tcgcgactcg ctggcgattt ttggtaacga ttatccgacc gaagacggta ctggcgtgcg
    40861 cgattacatc cacgtaatgg atctggcgga cggtcacgtc gtggcgatgg aaaaactggc
    40921 taacaagcca ggcgtacaca tctacaatct cggcgctggc gtaggcagca gcgtgctgga
    40981 tgtcgtcaat gccttcagca aagcctgcgg caaaccggtt aactatcatt ttgcaccgcg
    41041 tcgcgagggc gatcttccgg cctactgggc ggacgccagc aaagccgatc gcgaactgaa
    41101 ctggcgcgta acgcgcacac tcgatgaaat ggcgcaggac acctggcact ggcagtcacg
    41161 ccatccacag ggatatcccg attaaggaac gaccatgacg caatttaatc ccgttgatca
    41221 tccacatcgc cgctacaacc cgctcaccgg gcaatggatt ctggtttcac cgcaccgcgc
    41281 taagcgcccc tggcaggggg cgcaggaaac gccagccaaa caggtgttac ctgcgcacga
    41341 tccagattgc ttcctctgcg caggtaatgt gcgggtgaca ggcgataaaa accccgatta
    41401 caccgggact tacgttttca ctaatgactt tgcggcctta atgtctgaca cgccagatgc
    41461 gccagaaagc aacgatcctt tgatgcgttg ccagagcgcg cgcggtacca gccgggtgat
    41521 ctgcttttca ccagatcaca gtaaaacgct gccagagcta agtgttgcgg cattgacgga
    41581 aatcgtcaaa acctggcagg agcaaaccgc agaactgggg aaaacgtacc catgggtgca
    41641 ggtctttgaa aacaaaggcg cggcgatggg ctgctctaac ccgcatccgc acggacaggt
    41701 ttgggcaaat agcttcctgc ctaacgaagc tgagcgcgaa gaccgcctgc aaaaagaata
    41761 ttttgccgag cagaaatcac caatgctggt ggattatgtt cagcgcgagc tggcagacgg
    41821 tagccgtacc gttgtcgaaa ccgaacactg gttagccgta gtgccttact gggctgcctg
    41881 gccgttcgaa acgctactgc tgcccaaagc ccacgtttta cggatcaccg atttgaccga
    41941 cgcccagcgc agcgatctgg cgctggcgtt gaaaaagctg accagtcgtt atgacaacct
    42001 cttccagtgc tccttcccct actctatggg ctggcacggc gcgccattta atggcgaaga
    42061 gaatcaacac tggcagctgc acgcgcactt ttatccgcct ctgttgcgct ccgccaccgt
    42121 acgtaaattt atggttggtt atgaaatgct ggcagagacc cagcgagacc tgaccgcaga
    42181 acaggcagca gagcgtttgc gcgcagtcag cgatatccat tttcgcgaat ccggagtgta
    42241 agaaatgagt ctgaaagaaa aaacgcaatc tctgtttgcc aacgcatttg gctaccctgc
    42301 cactcatacc attcaggcgc ctggccgcgt gaatttgatt ggtgaacaca ccgactacaa
    42361 cgacggtttc gttctgccct gcgcgattga ttatcaaacc gagatcagct gtgcgccacg
    42421 cgatgaccgt aaagttcgcg taatggcagc cgattatgaa aatcagcttg atgagttttc
    42481 ccttgatgcg cccattgtcg ctcatgaaaa ctatcaatgg gctaactacg ttcgtggcgt
    42541 ggtgaaacat ctgcaactgc gtaacaacaa cttcggcggc gtggacatgg tgatcagcgg
    42601 caacgtgccg cagggtgccg ggttaagttc ttccgcctca ctggaagtgg cggtcggaac
    42661 cgtattgcag cagctttatc atctaccgct ggacggcgca caaatcgcgc ttaacggtca
    42721 ggaagcagaa aaccagtttg ttggctgtaa ctgcgggatc atggatcaac ttatttccgc
    42781 gctcggcaaa aaagatcacg ccttgctgat tgactgccgc tcactgggta ccaaagcagt
    42841 ttccatgccg aaaggtgtgg ctgtggtcat catcaacagt aacttcaaac gtaccctggt
    42901 tggcagcgaa tacaacaccc gtcgtgaaca gtgcgaaacc ggtgcgcgtt tcttccagca
    42961 gccagccctg cgcgatgtca ccattgaaga gttcaacgct gttgcgcatg aactggaccc
    43021 aatcgtggcg aaacgcgtgc gtcatatcct gactgaaaac gcccgcaccg ttgaagctgc
    43081 cagcgcgctg gagcaaggcg acctgaaacg tatgggcgag ttgatggcgg agtctcatgc
    43141 ctctatgcgc gatgatttcg aaatcaccgt gccgcaaatt gacactctgg tagaaatcgt
    43201 caaagctgtg attggcgaca aaggtggcgt acgcatgacc ggcggcggat ttggcggctg
    43261 tatcgtcgcg ctgatcccgg aagagctggt gcctgccgta cagcaagctg tcgctgaaca
    43321 atatgaagca aaaacaggta ttaaagagac tttttacgtt tgtaaaccat cacaaggagc
    43381 aggacagtgc tgaacgaaac tcccgcactg gcacccgatg gtcagccgta ccgactgtta
    43441 actttgcgta acaacgcagg gatggtagtc acgctgatgg actggggtgc gactttactt
    43501 tcagcccgta ttccgctttc cgatggcagc gtccgcgagg cgctgctcgg ctgtgccagc
    43561 ccggaatgct atcaggatca ggccgcgttt ctgggggcct ctattggtcg ttatgctaac
    43621 cgcatcgcca atagccgtta tacctttgac ggtgaaaccg tgacgctgtt gccaagtcag
    43681 ggcgtcaacc aattgcacgg cggcccggaa gggttcgaca aacgtcgctg gcagattgtg
    43741 aaccagaacg atcgtcaggt gctgtttgcc ctgagttcag atgatggcga tcagggcttc
    43801 ccgggtaatc tcggcgcgac ggtgcaatat cgtctgaccg acgataaccg tatctccatt
    43861 acttatcgcg ccacagttga taaaccttgc ccggtgaata tgactaatca cgtctatttc
    43921 aatcttgacg gcgagcagtc tgacgtgcgc aatcacaagt tgcagattct ggcggacgaa
    43981 tatctgccgg ttgatgaagg cggcattccg cacgacggcc tgaaatctgt cgccggaacg
    44041 tcttttgatt tccgcagcgc caaaatcatc gccagtgagt ttcttgccga cgacgatcag
    44101 cgcaaagtga aaggttacga tcacgcattc ttgttacagg ccaaaggcaa tggcaagaaa
    44161 gtggcggcgc atgtctggtc agcagatgaa aaattgcagc tgaaggtcta caccaccgct
    44221 ccggctctgc aattctactc cggcaacttc ctcggcggca caccgtcgcg gggaaccgaa
    44281 ccttacgccg actggcaagg gctggcactg gaaagcgagt ttctgccgga cagcccgaac
    44341 caccctgaat ggccgcaacc ggactgcttc ctgcatcctg gtgaagagta ttccagcctg
    44401 acggaatatc agtttattgc tgagtaagtt taagccctcg cgatgagggc tttccccttg
    44461 tattgttcaa atcaccagca aacaccgaca tatttgcaac tcaatattca caacaacctt
    44521 acactgcgac actattttcg ctatggttat gcgtaagcat tgctgttgct tcgtcacagc
    44581 aatataatga gaattattat cattaaaaga tgatagatga tttgaggagt aagtatatgg
    44641 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    44701 tcaccggttg gtacgacgtg gatctttctg agaaaggcgt aagcgaagca aaagcagcag
    44761 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    44821 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    44881 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    44941 aaaccgccga aaagtatggc gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga
    45001 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga
    45061 aactgagcga gaaagagctg ccgctgactg aaagcctggc gctgaccatt gaccgcgtga
    45121 tcccttactg gaatgaaact attctaccgc gtatgaagag cggtgagcgc gtaatcatcg
    45181 ccgcacacgg taactctctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag
    45241 agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt
    45301 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcagcg aaagcagcgg
    45361 cggttgctaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaatgag aaagccgact
    45421 gcaagtgagt cggctttttt gttgctaaca atggagcaca ttgcctgatg cggcgctgcg
    45481 cgtcttatca ggcctacggt gattcatcgg atacgccact ctggcggtgc atccgacaat
    45541 taaaccttac ccgcgacgcg cttttactgc attcgccagt tgacgtaaca gagcatcggt
    45601 atcttcccag ccaatgcagg catcagtgat gctcttaccg taggccagcg gctccccgct
    45661 ctccaggctc tgattgcctt caaccagatg actttccacc atcacgccaa taatggcctt
    45721 ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca tccatctgct ttttgaattg
    45781 ttttgacgag ttagcatggc tgaaatcgat catcacctgt gctggcaggc ctgctttgtt
    45841 cagcccttct ttcacttcag caacgtgctt cgcgctgtag ttaggctctt taccgccgcg
    45901 cagaatgata tggcaatcgc cgttaccgct ggtattcaca atcgccgaat gcccccattt
    45961 cgttacggac aggaagcagt gcggcgcacc ggcggcatta atggcatcga tagccacttt
    46021 aatcgtaccg tcggtgccat ttttgaagcc gaccggacaa gaaagccctg atgccagttc
    46081 gcggtgcacc tgcgattcgg tggtacgtgc gccaattgcg ccccagctca tcaggtcagc
    46141 gagatattgt ggggtgatca tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt
    46201 aatatcaagc agcaatttac gggcaatacg caaaccgtcg ttgatctgga agctattgtc
    46261 catatgcgga tcgttaatca gccctttcca gcccaccgtg gtgcgcggct tttcaaaata
    46321 gacgcgcatt acgatttcca gctcatcttt cagctcttca cgcagcgcca gcaagcgagt
    46381 ggcgtactct ttagccgcta caggatcatg aattgagcac ggaccaatca ccactaacag
    46441 gcgatcatca ttccctttca ggattttatg gatcgctttt cgggcatggg caaccgtatt
    46501 agcggcattt tcagtagcgg ggaatttttc cagcaatgcg acaggaggaa gtaactcttt
    46561 gatttctttg atgcgtaaat cgtcgttctg ataattcatg tctgttccag tgttgccata
    46621 cttatcttag tgaatgcaat acctccaatc tatatcttcc gtcagaatgt gtaaacgggg
    46681 ttttacacta tgaacggatt aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa
    46741 aaacgagatc tctcctacaa tttctaactg taactccttt gtttgttaat tatttcaaga
    46801 ttctctgctg gatttctttg cctggctgaa atcttaaacc aatgccttat attcacctgc
    46861 aaatgcactg ttggaagagg ttatccgaca taacgaccat aacaggagca tcctatgaaa
    46921 atgacaaaac tggccacatt atttctgact gccactctaa gccttgccag cggtgccgca
    46981 ctggctgccg atagcggagc gcaaactaat aacggccagg caaacgccgc cgctgatgcg
    47041 ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg
    47101 gtaaatacgg gttctggcgg cacaatgctg catccggatg gttcttcaat gaacaatgac
    47161 ggcatgacca aagatgaaga gcacaagaac accatgtgca aagatggtcg ctgtccggat
    47221 atcaataaaa aggtacaaac cggtgatggc atcaataatg atgtcgatac caaaaccgac
    47281 ggtaccacgc agtaaccctg caaaatagcc aataatggga gagcggagtc tctccctttt
    47341 aatttttccc ttctaaaagc agtatgatgt cataaagtta agtgagaata attaagggat
    47401 gactcgtcat ggcgcactca cactcacaca cgtcctcaca cctgccagaa gataataatg
    47461 ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt tatgctggta gaagtcattg
    47521 gtggttttct ttctggttct ctggctttgc tggccgatgc gggtcatatg ttgaccgata
    47581 ctgccgccct gcttttcgcc ctactcgccg tgcaattttc acgtcgtcct cccaccattc
    47641 gccacacttt cggttggcta agactgacca cgctcgcggc gtttgtgaat gccatcgccc
    47701 tcgtggtaat taccattttg attgtctggg aggcgataga acgcttccgc acgccgcgtc
    47761 cggtcgaggg cggcatgatg atggcaattg ccgtggctgg gttgctggca aatatacttt
    47821 ctttctggtt acttcatcac ggcagcgaag agaaaaacct caacgtacga gcagcggcac
    47881 tgcatgtgct cggggacctg ctgggttcgg ttggggctat tattgccgcg ttgattatta
    47941 tctggaccgg ctggacacct gctgacccca ttctctcgat actggtgtcg cttctggttc
    48001 tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga attacttgag ggtgcaccgg
    48061 tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca
    48121 atgtgcacca tgtgcatgta tggatggtag gcgagaagcc ggtgatgacg ttgcatgtgc
    48181 aggtgatccc gccacacgat cacgatgcct tgttggatca gatccaacac tacctgatgg
    48241 atcactatca gattgagcac gccaccattc agatggaata tcaaccttgt catgggccag
    48301 actgccacct gaatgaaggg gtgtctggcc attcacatca tcaccattaa tgggacagcg
    48361 cgcgtgagcc tctttcacgt gcgctgttga tccacatccg gctgccgttg agcgcaataa
    48421 aggtcaggat gatgtactcc agagacatgg cgtaaacgcc ctgaagtgca aaaataacga
    48481 cgctaatcac gttaataatc acccacaaca gccagttttc cacatactta cgcgtcatca
    48541 gaatcattgc cacgatagat aacaccatca tgcatgaatc ccagaacggg aaagcgtccg
    48601 gttgcagttc aggcatcaca acctgtaatc ctaatgcttg catgatgatg actgccacgc
    48661 gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat cagaccaata gcaatgacac
    48721 aaaccgccag ccaggttaat gccttcggca atggtaacca gcgaattttc aactccgcct
    48781 cgttctgact ggtttgtcgc gaccacgcat accagccgta aatattcgcg gcaaagaaaa
    48841 acacctgtaa tagcaggctg gcatacagct gaatctgaaa gaaaataatg ccaaacaagg
    48901 tgacgttgat caggccaaag aagtagttgc tgatcttctc cagactggca aggccaatac
    48961 acagcaaccc ggcgatcgtg cctaccgctt cgatccatga gagatcataa ccgcctgccc
    49021 ctattggtat atgtaccagg atattctgca cactaaaaaa atccatcttt cccccaaagc
    49081 gtaacgttac gccttattat tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga
    49141 ttgagcggca ccagcgccct ctctcgcagc ctctcatcaa catgaacctc gtgattactt
    49201 ccttcctgtt ctaatgcctc agcgatagcc tgaaggccat tcatggccat ccacggacaa
    49261 tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg gtgcttccag taactcttta
    49321 tctggcaccg cctgctgcat tttgtagaaa atgccccgat cggttgccac gataagccgc
    49381 tgatgtggca atgtttttgc agcagcgatc agttgactgg tggaaccgac cgcatccgcc
    49441 atatcgacaa tagcttgtgg tgattctgga tgcaccagta tggcagcatc cgggtattct
    49501 tcttgcaagc gggttaacgc ctgagtctta aattcatcat gcacaatgca agcaccctgc
    49561 cagcataaaa tgtctgcgcc cgtctgtttt tgcacgtaac gccccagatg tttgtcgggt
    49621 gcccagatga ttttttcacc caaactatca agatgatcaa taagttcgac ggcaatgctt
    49681 gaagttacca cccaatctgc gcgcgctttt accgcagcag aagtgttggc gtagacgacg
    49741 acagtacgat cgggatgggc atcgcaaaat gcgttaaatt cttcaacagg gcagccgaga
    49801 tccagtgaac actcagcctg aagtgtcggc atcagaattg ttttttccgg actgagaatt
    49861 ttggcggttt ctcccataaa tctcacccca gcgactaaca acgtagaagc gggatgcttt
    49921 gcaccgaagc gcgccatttc caaagaatca gaaatacagc caccggtttc ttctgccagt
    49981 tgttgaattt cgggatcggt atagtagtgg gcaaccatca ccgcattacg ttcttttagc
    50041 agacgttcta tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg cgtcggcttc
    50101 ggggggaaag gataaatcgc cgtgtctgga tcaaacatca cgctcatctt accatctcgt
    50161 tttacaggct taacgttaaa accgacatta gcgtgatatt cgctgttatc ggtgtaattt
    50221 gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa ttacgtatga
    50281 gatgccgaat gccacacttt gcgtcttatt cctccgacgt aaagttgttt acgcagcaag
    50341 cgcggagtat gtcatcaggg tcaagaattg aaattgagag atgcctg
//
LOCUS       DAZFFG010000020        49026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFFG010000020 DAZFFG010000000
VERSION     DAZFFG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.020"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(12..332)
                     /gene="chbB"
                     /locus_tag="ACHJ7J_001520"
     CDS             complement(12..332)
                     /gene="chbB"
                     /locus_tag="ACHJ7J_001520"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO4999772.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(632..970)
                     /gene="osmE"
                     /locus_tag="ACHJ7J_001521"
     CDS             complement(632..970)
                     /gene="osmE"
                     /locus_tag="ACHJ7J_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO4999773.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1172..1999
                     /gene="nadE"
                     /locus_tag="ACHJ7J_001522"
     CDS             1172..1999
                     /gene="nadE"
                     /locus_tag="ACHJ7J_001522"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO4999774.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETDNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2229..3116
                     /gene="cho"
                     /locus_tag="ACHJ7J_001523"
     CDS             2229..3116
                     /gene="cho"
                     /locus_tag="ACHJ7J_001523"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO4999775.1"
                     /translation="MVRRLPSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSQAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3076..3651)
                     /gene="ves"
                     /locus_tag="ACHJ7J_001524"
     CDS             complement(3076..3651)
                     /gene="ves"
                     /locus_tag="ACHJ7J_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO4999776.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3854..4339)
                     /gene="spy"
                     /locus_tag="ACHJ7J_001525"
     CDS             complement(3854..4339)
                     /gene="spy"
                     /locus_tag="ACHJ7J_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO4999777.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4669..5637)
                     /gene="astE"
                     /locus_tag="ACHJ7J_001526"
     CDS             complement(4669..5637)
                     /gene="astE"
                     /locus_tag="ACHJ7J_001526"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO4999778.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5630..6973)
                     /gene="astB"
                     /locus_tag="ACHJ7J_001527"
     CDS             complement(5630..6973)
                     /gene="astB"
                     /locus_tag="ACHJ7J_001527"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO4999779.1"
                     /translation="MNAWEVNFDGLVGLTHNYAGLSFGNEASTSHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQAQLLAN
                     LRARVNSFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMVLVLPQECREHAGVWCY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6970..8448)
                     /gene="astD"
                     /locus_tag="ACHJ7J_001528"
     CDS             complement(6970..8448)
                     /gene="astD"
                     /locus_tag="ACHJ7J_001528"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO4999780.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSDEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVAGIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPEREKFEQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8445..9479)
                     /gene="astA"
                     /locus_tag="ACHJ7J_001529"
     CDS             complement(8445..9479)
                     /gene="astA"
                     /locus_tag="ACHJ7J_001529"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO4999781.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9476..10696)
                     /gene="astC"
                     /locus_tag="ACHJ7J_001530"
     CDS             complement(9476..10696)
                     /gene="astC"
                     /locus_tag="ACHJ7J_001530"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO4999782.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACERFVSGGSS"
     gene            11142..11948
                     /gene="xthA"
                     /locus_tag="ACHJ7J_001531"
     CDS             11142..11948
                     /gene="xthA"
                     /locus_tag="ACHJ7J_001531"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO4999783.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12115..12825
                     /gene="ydjX"
                     /locus_tag="ACHJ7J_001532"
     CDS             12115..12825
                     /gene="ydjX"
                     /locus_tag="ACHJ7J_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4999784.1"
                     /translation="MNTERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPHLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISSLTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRHSPLTHPKNEG"
     gene            12830..13507
                     /gene="ydjY"
                     /locus_tag="ACHJ7J_001533"
     CDS             12830..13507
                     /gene="ydjY"
                     /locus_tag="ACHJ7J_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO4999785.1"
                     /translation="MLQHYSVPWKKGLAALCLLAVAGLSGCDQKESTAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13522..14229
                     /gene="ydjZ"
                     /locus_tag="ACHJ7J_001534"
     CDS             13522..14229
                     /gene="ydjZ"
                     /locus_tag="ACHJ7J_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4999786.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINSSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14229..14777
                     /gene="ynjA"
                     /locus_tag="ACHJ7J_001535"
     CDS             14229..14777
                     /gene="ynjA"
                     /locus_tag="ACHJ7J_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4999787.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14787..15953
                     /gene="ynjB"
                     /locus_tag="ACHJ7J_001536"
     CDS             14787..15953
                     /gene="ynjB"
                     /locus_tag="ACHJ7J_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4999788.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKSGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTTPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15926..17461
                     /gene="ynjC"
                     /locus_tag="ACHJ7J_001537"
     CDS             15926..17461
                     /gene="ynjC"
                     /locus_tag="ACHJ7J_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
                     /protein_id="HFO4999789.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRRQCLNWQLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDANQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALQLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17461..18114
                     /gene="ynjD"
                     /locus_tag="ACHJ7J_001538"
     CDS             17461..18114
                     /gene="ynjD"
                     /locus_tag="ACHJ7J_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4999790.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18181..19488
                     /gene="ynjE"
                     /locus_tag="ACHJ7J_001539"
     CDS             18181..19488
                     /gene="ynjE"
                     /locus_tag="ACHJ7J_001539"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO4999791.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLFLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMDQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDIAAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19497..20117)
                     /gene="ynjF"
                     /locus_tag="ACHJ7J_001540"
     CDS             complement(19497..20117)
                     /gene="ynjF"
                     /locus_tag="ACHJ7J_001540"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4999792.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20204..20611
                     /gene="nudG"
                     /locus_tag="ACHJ7J_001541"
     CDS             20204..20611
                     /gene="nudG"
                     /locus_tag="ACHJ7J_001541"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO4999793.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREISGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20577..20849)
                     /gene="ynjH"
                     /locus_tag="ACHJ7J_001542"
     CDS             complement(20577..20849)
                     /gene="ynjH"
                     /locus_tag="ACHJ7J_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO4999794.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLNTNPLVWQRVKP"
     gene            21085..22428
                     /gene="gdhA"
                     /locus_tag="ACHJ7J_001543"
     CDS             21085..22428
                     /gene="gdhA"
                     /locus_tag="ACHJ7J_001543"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO4999795.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGDEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22545..23585)
                     /gene="ynjI"
                     /locus_tag="ACHJ7J_001544"
     CDS             complement(22545..23585)
                     /gene="ynjI"
                     /locus_tag="ACHJ7J_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4999796.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSINI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDDQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23712..25673)
                     /gene="topB"
                     /locus_tag="ACHJ7J_001545"
     CDS             complement(23712..25673)
                     /gene="topB"
                     /locus_tag="ACHJ7J_001545"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO4999797.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(25678..26721)
                     /gene="selD"
                     /locus_tag="ACHJ7J_001546"
     CDS             complement(25678..26721)
                     /gene="selD"
                     /locus_tag="ACHJ7J_001546"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO4999798.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(26838..27389)
                     /gene="ydjA"
                     /locus_tag="ACHJ7J_001547"
     CDS             complement(26838..27389)
                     /gene="ydjA"
                     /locus_tag="ACHJ7J_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO4999799.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27550..29406
                     /gene="sppA"
                     /locus_tag="ACHJ7J_001548"
     CDS             27550..29406
                     /gene="sppA"
                     /locus_tag="ACHJ7J_001548"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO4999800.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASIVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29560..30576
                     /gene="ansA"
                     /locus_tag="ACHJ7J_001549"
     CDS             29560..30576
                     /gene="ansA"
                     /locus_tag="ACHJ7J_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO4999801.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            30587..31228
                     /gene="pncA"
                     /locus_tag="ACHJ7J_001550"
     CDS             30587..31228
                     /gene="pncA"
                     /locus_tag="ACHJ7J_001550"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO4999802.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(31321..32679)
                     /gene="ydjE"
                     /locus_tag="ACHJ7J_001551"
     CDS             complement(31321..32679)
                     /gene="ydjE"
                     /locus_tag="ACHJ7J_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4999803.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWLLSGKYFIESPRWLAGKGQIAGAECQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(32797..33555)
                     /gene="ydjF"
                     /locus_tag="ACHJ7J_001552"
     CDS             complement(32797..33555)
                     /gene="ydjF"
                     /locus_tag="ACHJ7J_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4999804.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(33692..34672)
                     /gene="ydjG"
                     /locus_tag="ACHJ7J_001553"
     CDS             complement(33692..34672)
                     /gene="ydjG"
                     /locus_tag="ACHJ7J_001553"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO4999805.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIHAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(34682..35629)
                     /gene="ydjH"
                     /locus_tag="ACHJ7J_001554"
     CDS             complement(34682..35629)
                     /gene="ydjH"
                     /locus_tag="ACHJ7J_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4999806.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRVGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(35634..36470)
                     /gene="ydjI"
                     /locus_tag="ACHJ7J_001555"
     CDS             complement(35634..36470)
                     /gene="ydjI"
                     /locus_tag="ACHJ7J_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO4999807.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(36491..37534)
                     /gene="ydjJ"
                     /locus_tag="ACHJ7J_001556"
     CDS             complement(36491..37534)
                     /gene="ydjJ"
                     /locus_tag="ACHJ7J_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO4999808.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGSGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(37551..38930)
                     /gene="ydjK"
                     /locus_tag="ACHJ7J_001557"
     CDS             complement(37551..38930)
                     /gene="ydjK"
                     /locus_tag="ACHJ7J_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4999809.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIHQTGKPLPPVVIADDGKTPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(38957..40033)
                     /gene="ydjL"
                     /locus_tag="ACHJ7J_001558"
     CDS             complement(38957..40033)
                     /gene="ydjL"
                     /locus_tag="ACHJ7J_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4999810.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPMCGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(40403..40675)
                     /gene="yeaC"
                     /locus_tag="ACHJ7J_001559"
     CDS             complement(40403..40675)
                     /gene="yeaC"
                     /locus_tag="ACHJ7J_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO4999811.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(40717..41130)
                     /gene="msrB"
                     /locus_tag="ACHJ7J_001560"
     CDS             complement(40717..41130)
                     /gene="msrB"
                     /locus_tag="ACHJ7J_001560"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO4999812.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            41472..42467
                     /gene="gapA"
                     /locus_tag="ACHJ7J_001561"
     CDS             41472..42467
                     /gene="gapA"
                     /locus_tag="ACHJ7J_001561"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4999813.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            42551..43435
                     /gene="yeaD"
                     /locus_tag="ACHJ7J_001562"
     CDS             42551..43435
                     /gene="yeaD"
                     /locus_tag="ACHJ7J_001562"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO4999814.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(43486..44340)
                     /gene="yeaE"
                     /locus_tag="ACHJ7J_001563"
     CDS             complement(43486..44340)
                     /gene="yeaE"
                     /locus_tag="ACHJ7J_001563"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO4999815.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMENLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(44430..45176)
                     /gene="mipA"
                     /locus_tag="ACHJ7J_001564"
     CDS             complement(44430..45176)
                     /gene="mipA"
                     /locus_tag="ACHJ7J_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO4999816.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            45612..47546
                     /gene="yeaG"
                     /locus_tag="ACHJ7J_001565"
     CDS             45612..47546
                     /gene="yeaG"
                     /locus_tag="ACHJ7J_001565"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO4999817.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            47661..48944
                     /gene="yeaH"
                     /locus_tag="ACHJ7J_001566"
     CDS             47661..48944
                     /gene="yeaH"
                     /locus_tag="ACHJ7J_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO4999818.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        12176 a        12799 c        11930 g        12121 t
ORIGIN      
        1 tttaaaataa attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg
       61 cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt
      121 aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg
      181 gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact
      241 tcatattttt ctgcctgtgc gcgcattttt gataccagta aagaggtaga catgcccgca
      301 gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt
      361 cagccagaca ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa
      421 catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt
      481 tgaccttata aattgtctct ccttgacctg cggttatgac cctcttcaca tttcgggcaa
      541 ataattcgcg aagataatta agaactcagg ccaaaaaaaa accggcgcaa tggccggttt
      601 ccgttgttac tcaagctttc agactaagtg attacttcgc agcctgtgga tcagtgtcgt
      661 attcagcaca ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa
      721 agtaggtttc tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccacgag
      781 catggatcat gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc
      841 tcatgccttt tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat
      901 aagccgtaca acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat
      961 tcttgttcat attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg
     1021 gcgctttttc aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt
     1081 ttaaccacca ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg
     1141 aagttttgtc ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc
     1201 gctgggcgca aaaccgcaga ttaatgctga agaggaaatt cgtcgtagta tcgattttct
     1261 gaaaagctac ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg
     1321 tcaggactcc acgcttgccg gaaagctgtg ccagatggcg attaatgagc tgcgccagga
     1381 aaccgacaac gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggccga
     1441 cgaacaagat tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa
     1501 tatcaagggc gcggtattgg ctagcgagca ggcattgcgg gaagcaggca ttgaactgag
     1561 cgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc
     1621 gggtatgacc agcggtgtcg tggtgggcac cgatcatgca gcagaagcca ttaccggatt
     1681 cttcactaaa tatggtgacg gcggtacgga cattaatccg ctgtatcgtc tcaacaaacg
     1741 tcagggtaaa cagttactgg cggcattagg ttgcccggaa cacctttata agaaagcgcc
     1801 aacggccgat ctggaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac
     1861 ctatgacaat atcgacgact atctggaagg gaaaaacgta cctgaacagg tcgccagaac
     1921 aatagagaac tggtatctga aaaccgaaca taaacgccgt ccgccaatta ccgttttcga
     1981 tgatttctgg aaaaagtaat aatttgcaca tattggattg tgcgaaaaag agtaatttgt
     2041 tcacgccgga tgcggcgtga acgccttatt cgacctacaa aactatgcag attcaataca
     2101 ttgcaggagt cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc
     2161 gacccgcctc ttttttaatc gcttcccgcc tgttacactg gatagataac cagcattcgg
     2221 agtcaacagt ggtacggcgt ttaccttctc cgcggctcga atttgaagct gcggcaattt
     2281 atgaatatcc cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt
     2341 atctgtttca tggcgaaagt gacaccatgc cgctctatat cggcaaaagc gttaatatcc
     2401 gcagtcgggt gctttctcat ttacgcaccc ctgatgaagc cgccatgcta cggcaatccc
     2461 ggcgaattag ctggatctgc accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat
     2521 taatcaaaga acaacagccg ctgtttaata aacggttgcg ccgcaatcgc cagctctgtg
     2581 ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac
     2641 aagcccccaa cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga
     2701 ccatcgctga tgaacaaaaa ctttgttatg gcctgctggg actggaaccg ttaagtcgcg
     2761 gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga
     2821 gccatgagga acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt
     2881 ggccttggca aggggcggtg gcgctgaaag aacagcaccc ggaaatgact caatatcata
     2941 ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg acaacgttaa
     3001 ttcggacacc cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt
     3061 ccggtaacta tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc
     3121 actgaacaac agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca
     3181 gcacgcgccc tggtcggtag tgagcagttt gtcgcccagt tgccacgccc cgttaatcac
     3241 aaacaccacc ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt
     3301 cgccttacag acatccaggc gcgtcataat attgaaatcc atcgacatct gccctgccgt
     3361 cagtttcgct tttaccacct ggtccgccgc aaaggcaaaa ggctgtaacg gttttaaggt
     3421 atggttaaag tggtctgcgc tttcaaggaa catctcgccg ccttccagca acgtcactat
     3481 cctttccata ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc
     3541 acgccagtaa aaatcacgtt ttgccggtgg gaaagtgcaa atttctcgcg tttcaccggc
     3601 agcgtttcgc cacagattta ccgacatttt acgcatatca aagtattcca tactcgctcc
     3661 cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg
     3721 tgatttacgt tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac
     3781 cgccgatcct gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt
     3841 tttggctgaa agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg
     3901 tcagacgctt ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt
     3961 aaattttgtt ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt
     4021 ttgcgatctg cgcttcagct tttgctttat cgaaggtatc gctggcaatg atgtcatgca
     4081 ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt
     4141 cgcggatctg ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat
     4201 gcggaccgaa cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggtag
     4261 tggtgtctgc ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca
     4321 gtgcagttaa tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt
     4381 aagtagcgtg ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc
     4441 aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa
     4501 aatttgatgg agagtgatga gcgaatatta caacacgatg attttgcaga gattatgaag
     4561 aactataccg gatgactggt gataaataaa gcaaataacc aggattaatc tgtattaatt
     4621 tataagaaag caacttaata cccgcagaat gatttctgcg ggtaagtatt agcttatttt
     4681 ttcgagcatt aatcccgcgc gtaatcccaa cgctaccaac ggattaggga ataacacata
     4741 ctctacatca tgggttacgg taaaacgttc ctctccgtcc tgcgccagca atgttccttt
     4801 ctcaaacggc ataaaattca gcgtgtcact tgccatatgc atttcgaagg acggcgagtg
     4861 gcgagtaatt tgcgaaacca cccgataacg gagcggcggt gttctcacga taccgacact
     4921 ctcaccagat agcagcgcag caattgcgct ggcagttact gcaaactggc gaagatcgtt
     4981 ttgtccaaag ggcaacgctt tgccgagttc cagcgtacag gccagcgcgc caaaatgtct
     5041 ggcgctgaaa tgggtaaacg taccaccagg ctcctgatgg aacaccagtg cctccagacc
     5101 cgccgcaccc agccacgtca ggaatttctc gtcccagggt atgtcgcgtt gcggtaatac
     5161 accgaactgc ggatgcaagg agccacgaat tgcggtatgt agatcaaggt gccagcgcac
     5221 agattcttta ccctggtcat aaaaatcttc caggcactgt tccagttcgc gcgcccgaca
     5281 ggtttccccg ctttcagcaa acagctgcca acgaccgcca aacattcggt tcatatcgct
     5341 atggcaataa cgtttccctt gcttcagcgc aggaggattc ccgaggatta ccagcaaccg
     5401 ccaacgtaac gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca gcatctccac
     5461 aggtgccgtc tcattaccgt gtattcccgc tgaaatcacc aacgcgcctt gcggtggcgt
     5521 taatggcgtc agttccagca caccatcgcc cagccagcgc cagcgaacgc cgttgatttc
     5581 tcgctcggtg ataaccggtt ttttacccgt taaggtcaga gcaagaaaat tatccattcc
     5641 cccctccctc gcgctggaac ggataaaccg aaccgagatt cagtaattga ctcaatacat
     5701 ccagtgcttc ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca gcagtaagac
     5761 gatcgcggta gtaacgatcc acccagtcat tgagcgcatt aaacaaggta tcgttcatca
     5821 tcaccgccgg attcaccgcc cggcgttctt cttgtgtcaa taccacccgc aaccgcaggc
     5881 acgccgggcc gccgccattc gccatgcttt cacgtaaatc aaagactttt agttcgctaa
     5941 tcgggttgtc agcggcaagg agttcattga gataacacca tactccggcg tgttcccgac
     6001 actcctgtgg cagcaccaac accatggaac catcatcgcg gctcagcagt tggctgttga
     6061 acagatatgt agacaccgca tccgacacgg aaacctgcgc cgtgggcacc tctattgcca
     6121 taaagctatt gacccgagca cgcaggtttg ccagtaactg cgcctgtcga gcgaacgcct
     6181 gttgatggca aaacagcacc tggcggttac tcacggcaat cacgtcatta tgaaaaacgc
     6241 cctggtcgat aacgtccggg ttttgctggg cgaaaatcac ctgttgggga ttcacctgat
     6301 tcagccttgc caccgcctcg ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc
     6361 gggtatcatt gccttcttct cgcccgtaga caaaaagttg catacccggt tcgccataat
     6421 gaccgccgag acgattgtgg tttgctgccc cctcatcacc gagcaacgct acctgcggca
     6481 acgccgaatg aacgctaaat ttctcttcgt cgttaaaaat cgcttttaaa agtgattcgg
     6541 tgacgtgcgc ttccagcgag cggtgaaatt tattgttcag gttggcaacg gtgagatgca
     6601 ctttgccatc cagcgtatcg gcagatggcg cgatcgttgc cgcattggct acccacattg
     6661 gcgaagcgga gctgacgctg gaaagccagt gcggtgcctg gcgtgcaact ttttccagta
     6721 cctgctcatc gctgccgctg aatcccaact gacgcagcac cggaataaac ggacgctcgt
     6781 gcggcgggat caccgcctgg gggaatcccg catcggcaag ggctttcatt ttcagtaagc
     6841 cctgcttcgc cgccagtcgt ggattagata cctgaaaacg atggctggtg gaggcttcat
     6901 taccaaacga caggcccgcg taattgtgcg tcagccctac cagcccgtcg aaattgactt
     6961 cccaggcgtt catcgcacca cctcatcgga aaaatccagc ccggggttaa gcgttgcggg
     7021 caaagttaac gagtccgact ccaggctcgc cataggccat gcgcagtaat ctgcggcata
     7081 ccaggcgctg gggcgatggt taccggatgc accaatgccg ccgaatggcg cggtactggc
     7141 agcaccagta agcggtttgt tccagttaac aatccccgcc cgcgcctcca gcaacagttg
     7201 ttcgaatttt tcccgctcgg gggaaaccag cccacaagag aggccaaagc gagtgttatt
     7261 agccatcctg atcgcctcat cgaaggtgtc gtaacgccag acgcgcaata gcggcccgaa
     7321 cacttcttca tccggcacgc ctgcaacacc cgtcatttca ataatccccg gcgtcaacag
     7381 cgatgttccc gcttgcagca aacgcggcgc aagcagggtt cgtccgccca tcgcttccag
     7441 ttgctgccat gcagtaacca cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa
     7501 cggctgcggt tcgtcatccc agttgcccgg cgttaatcgc tggctgacgg caaccagacg
     7561 agcaagaaac gcatcgccct gcgccccgct tttcagcaat aaacggcggg cgcaggtgca
     7621 gcgttgaccg gcggtgacaa acgccgactg aatggtcaga tggacagcgg cgtcgatacc
     7681 cgccacctca tcgataatta gcggattatt accgcccatc tcaagggcga gaattttctc
     7741 cggctgaccg gagagctggc gatgcagctg gtagccggta ttggcgctac cggtaaacag
     7801 caaaccgtcg agatcctcca gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac
     7861 caggttcagc acgcccggcg gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc
     7921 actccacggt gtcagttcgc tgggtttaaa gataatggtg ttacctgcca gcaatgccgg
     7981 aacgatatgt ccgttcggca aatgaccagg gaaattatac ggcccaaaca ccgccagcac
     8041 gccgtgcggg cgatgtcgca ggctcgccgc gccgtccggc atctcgctac gctgctcgcc
     8101 ggtacgagcg tgatacgcct taattgatat cgcgatttta ttgatcatcg ccgtcacttc
     8161 ggttgccgct tcccagcgcg gcttgccggt ttctcgggca atgattgcgg ttaattcgcc
     8221 tttattcctt tccagcaatc cggcaaagcg ttcgacaacg acctgacgtt cagccaatga
     8281 gagccgcgcc cagcgcggaa acgccgcacg ggctgcccga caagcctgcc cgacctgagc
     8341 ggcatcggca tcattgcctt gccataacac ctcgtccgat accggattac gcttcacacg
     8401 cgatgcgccc tgacccgtta tccagtcacc gttaatccat aaagtcatgc tgttttctcc
     8461 tctgcgcaca ggcgcaccag acgaacgcga tcccctgcgt ggcatttgag ggcatccagt
     8521 tgtgcggcgg ttaaaatcaa acgctcggtt gccggatcgg tacgcaccag caccacgcgg
     8581 aaatggtgat aattttcatt ggcgaccagg caggccggaa aatcgccctg cgcaggctgc
     8641 ccttctgcca cttccaccag ccgactttta cggatggcgc gcacgcggtc gatgtcacac
     8701 tcaagcgtcg gcccaccgtc gaagatgtcg atatagttgc ggtaacgaaa accttctttc
     8761 tccagcaccg cgcgggcagg cgcggtttgc ggatgtacct gaccgatgac gtcctgggct
     8821 tcctgggata aaaagtgcgt atagatcgga tgtttcggca tcagttctgc aataaatgcc
     8881 ttttgcccgg tgccgcagag aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta
     8941 ccgaggcttt gccagaacgg tgaatagccg tgttcgtcaa tcaccccgcg catttcagca
     9001 accactttgt cattaaactt gtcgcgaaaa gccgccataa acataaagcg cgatttcgac
     9061 agcaaatagc cgttgccctc tttgcgccag tccgggtcga gaaacagcgt gcacagctcg
     9121 ctgctgccgg tgtgatcgtt actgagaaac agcgtcggca gcgcgttata gacattcagc
     9181 tctttcgaag cgtgaaccag cgtgccgacg cgatagttgt accagggatc gttcagccca
     9241 actgccacct caatggcaca aatccccgcc acggtgcctg tctcgctatc ttccagcacg
     9301 aacacatagc cctgctcact tttgggcagt tcgccttgcc aggttttgat tgccctttcg
     9361 atacgcgccg aaagcgtggc ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg
     9421 ctggcaagct gcatcagcgc cgagacatct gagcgttcaa cgggacggat gaccatcatg
     9481 atgaacctcc gctcacaaag cgttcacagg ccgcagcaaa gcgatccagt ccggtcgtca
     9541 cctcttcttc gctgacattg agcgcaggcg caaaacgcac cacgttgcca cccgcaatca
     9601 gtaccatcac gcctgctttc gccgcctcct gagagatctg tttcgcttgc ccggcgtaat
     9661 cggcattcag tacacagcca atcaaaagac ctaagccgcg aacttcactg aacaaaccgt
     9721 agcggtgatt aatggtatta agacgctcaa caaaccagtc gtgacgttgc ttaaccccgt
     9781 taagcatctc tggcgtattg atgagctcca gcactttgcc tgccaccgcc gaggccagcg
     9841 gattgccgcc ataggtggtg ccatgagtgc caacggtcat cacgctggcg cactcttcgg
     9901 ttgctaacaa cgcaccgacc gggaaaccgc cgcccagcgc tttggcggta gttaacagat
     9961 caggcgtcac gccgtagtgc atataggcat acagttcccc ggtgcgcccg acgccggttt
    10021 gtacttcatc aaaaatcaac agcgcattgt ggcgatcaca caattcacgc agaccttgta
    10081 aaaatgcgtt gctggctggc accacaccgc cttccccctg gatgggttcg acaatcaccg
    10141 cacaggtaga gtcgtcaatc agcgcgctgg cagagttaat atcgttatat gcagcatgac
    10201 ggatatctgg tggcagtggc gcaaaatcct gtgaataggc tggctgccca cccgcactaa
    10261 cagtaaacag cgtgcgacca tgaaacgcat ttttaaacgc cacgataccg ctcttatggc
    10321 tgccgtagcg gtcgtgagcg aatttacgcg ccagttttag cgccgcttca ttggcttccg
    10381 caccggagtt acaaaagaag acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca
    10441 gtcgcagtac cggctcgttg gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt
    10501 cgttcagcgc ctcacgcagt tccggatgcg catggcccag cgcgttcacc gcaatgccac
    10561 ccgcgaagtc gatatactct ttcccctgct gatcccacaa gcgcgaacct tcgccacgta
    10621 ccggtataaa cggtgccgga gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg
    10681 taattggctg agacatagcg acctctacag taaataattc gttatttata tgttaataat
    10741 aagtaatgtt tgcgctgtaa atgtagattg cagggttcgt gccagccagt gataaaagtg
    10801 cataaaccgc ggaggctaac tggaaatcaa ggagttataa ccaaaccata tgcatttaaa
    10861 gtgcatatta agtgaatacg tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg
    10921 ttatgcagaa gtaaaatata attctggaat tgtgatcatt gacgaaattt actggaaatt
    10981 actgcgccat tctgacgcag cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca
    11041 tcattaacaa ccatcgatca aatcacttaa caacaggcgg taagcaaagc gaaattctgc
    11101 taccatccac tcactcttta tctgaataaa tggcagcgac tatgaaattt gtctctttta
    11161 atatcaacgg cctgcgcgcc agaccccacc agcttgaagc catcgtcgaa aagcaccaac
    11221 cggatgtgat tggcctgcag gagacaaaag ttcatgacga tatgtttccg ctcgaagagg
    11281 tggcgaagct cggctacaac gtgttttatc acgggcagaa aggccattat ggcgtggcgc
    11341 tgctgaccaa agagacgccg attgccgtgc gtcgcggctt tcccggtgac gacgaagagg
    11401 cgcaacggcg gattattatg gcggaaatcc cctcgccgct gggcaatgtc accgtgatca
    11461 acggttactt cccgcagggt gaaagccgcg accatccgat aaaattcccg gcaaaggcgc
    11521 agttttatca gaatctgcaa aactatctgg aaaccgaact caagcgtgaa aatccggtgc
    11581 tgattatggg cgatatgaat atcagtcctg gcgacctgga tattggcatt ggcgaagaga
    11641 accgtaagcg ctggctgcgt accggaaaat gttctttcct tccggaggaa cgcgaatgga
    11701 tggagagact gatgagctgg ggattggttg ataccttccg ccatgcgaat ccccaaacag
    11761 cagatcgttt ctcatggttt gattaccgct caaaaggttt tgacgataac cgtggtcttc
    11821 gcatcgacct gctgctcgcc agccaaccgc tggcagaatg ttgcgtagaa accggcatcg
    11881 actatgaaat ccgcagcatg gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc
    11941 gccgctaatt tagcagctct cctggctcaa actgggtcag gagaattaac cttgagaaaa
    12001 atcaacaaac tgtcagtaat gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt
    12061 ttttagttgt ctgaccactt ctctattatc aagtttgata taggaaactc cacgatgaac
    12121 actgagcgta aatttctgtt tgcctgtctt atttttgcgc tggtcattta cgctatccac
    12181 gctttcggtt tattcgatct gctcaccgat ttaccccact tacagacact catccgccag
    12241 agcggattgt tcggttatag cctctatatt ctgttattca tcattgccac cctctttctg
    12301 ttaccaggaa gcatactggt gatcgcggga ggaatagttt ttggcccgca cttagggaca
    12361 ctactctcat taattgccgc cacgctggcc tcctcgtgct cattcctgct ggcgcgctgg
    12421 ctgggacgtg atttactgct gaaatacgtt ggccatagcc ataccttcca ggccattgaa
    12481 aaaggcattg cgcgtaacgg tattgatttt cttattctga cccgcttaat cccgttgttt
    12541 ccgtacaata ttcaaaatta cgcttacgga ttaacaacaa tcgccttctg gccttatacc
    12601 cttatttcgt cactcacgac cctgcccggt attgttattt ataccgtgat ggcaagcgat
    12661 ctcgccaatg aaggcattac actgcgcttt attttacaac tctgtctggc gggcctggcg
    12721 ctgtttattc tcgtccagct cgccaaactc tacgcccgac acaaacatgt ggatctgtct
    12781 gcttcgcgcc acagcccact tactcaccca aaaaatgaag gatagaacga tgttgcaaca
    12841 ttattcagtg ccatggaaaa aaggactggc tgcactctgc ttactggctg ttgctgggct
    12901 tagcggctgc gatcaaaaag agagtacggc ggcaaaagtg gaatacgacg gactttcgaa
    12961 cagccaaccg ctgcgcgtcg atgccaataa ccatacggta accatgctgg tgcaaattaa
    13021 tggtcgtttc ctcaccgacg acactcgtca cggtattgtg tttaaagatg gctccaacgg
    13081 acataaatcg ctgtttatgg gttatgcgac cccgaaagca ttttatgaag ccctgaaaga
    13141 ggcaggtggt acgccgggcg aaaacatgac gatggataat aaagaaacga ctcatgtcac
    13201 aggcagcaaa ctggatattt cggtcaactg gcaaggggcg gcaaaagcgt attccttcga
    13261 tgaagtgatt gttgacagta atggcaagaa actggacatg cgctttggcg gtaatttaac
    13321 ggcagcagaa gagaagaaaa cgggttgcct ggtgtgtctg gatagctgcc cggtcggcat
    13381 cgtcagcaat gcaacataca cttatggtgc ggttgaaaaa cgtggtgaag ttaaattcaa
    13441 aggcaatgcc tcagttctcc cggcggataa cacgctggca acggttacct ttaaaatcac
    13501 cgaataaagc caggataaag gatgatgaag atgcaatcgc gtaaaatctg gtactaccgt
    13561 ataaccctca tcatcctgtt gttcgctatg ctgctggcat gggcgctgct tcccggcgtc
    13621 catgagttta tcaatagcag cgttgcggcg tttgccgccg tggaccaaca gggtatagaa
    13681 cgctttattc agtcttacgg cgcactggcg gcagttgtct cgttcttgtt gatgatttta
    13741 caggccattg ctgcaccgct acctgcgttt ttgatcacct ttgccaatgc gtcgctgttt
    13801 ggcgcgttct ggggcggctt gctgtcgtgg accagttcga tggccggcgc ggcgctgtgc
    13861 ttttttatcg ccagagtgat gggccgcgaa gtggtggaaa aattaaccgg caaaaccgta
    13921 cttgacagta tggacggctt tttcactcgc tacggcaaac acaccatcct ggtctgtcgg
    13981 ttattgcctt ttgtcccttt cgatccaatc agctatgctg ccggtttgac ttcaatccgt
    14041 tttcgctcgt tttttatcgc caccgggctt ggtcagttac cggcgactat tgtttattcc
    14101 tgggcgggca gcatgttaac aggcggtacc ttctggtttg tcaccggact gtttattctg
    14161 tttgccctga ccgtggtgat ttttatggcg aagaaaatat ggcttgagcg ccagaagagg
    14221 aatgcctgat gggtttaccg ccgcttagca aaattccttt cattttacgt ccgcaggcgt
    14281 ggctgcatcg tcgccattac ggcgaggtgc taagccccat tcgctggtgg gggcggatcc
    14341 cgtttatctt ttatctggtg tcgatgtttg ttggctggct ggagcgcaaa cgctcaccgc
    14401 tcgatccggt agtacgatcg cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt
    14461 tttgtgtgga tatcaccagt atgaaagtcg ccgagcgcac cggcagcagc gataaactgc
    14521 tggcagtggc tgactggcgg caaagcccgc tctttagcga tgaagaacgg ctggcgctgg
    14581 agtacgccga agccgcaagc gtaacgccgc caacggtcga tgatgccctg cgtacccgac
    14641 tggctgcgca ttttgacgct caggcgctca ccgaactgac ggcattgatc ggcctgcaaa
    14701 acctgtcggc tcgttttaat tctgccatgg acattcccgc tcaggggctg tgccgtattc
    14761 cggaaaaacg ttcttaagga gagatgatgc gccattgtgg gtggttgctg ggattgttat
    14821 cgctgttttc tctggcaaca catgccagtg actggcaaga aattaaaaat gaggccaaag
    14881 ggcaaaccgt ctggtttaac gcctggggcg gcgataccgc aattaaccgc tatctcgact
    14941 gggtgagcgg cgagatgaaa acccattacg ctataaacct gaagattgtc cgtctggcgg
    15001 atgccgcaga cgcggtgaag cgcattcaga ccgaagccgc agccggacgt aaaacgggcg
    15061 gctcggtgga tctgctctgg gtgaacggcg aaaacttccg caccttaaaa gaggccaatt
    15121 tactgcaaac cgactgggca gagactctgc ccaactggcg ctatgtcgac acacagctgc
    15181 cggtgcggga agatttttca gtgcctacag aaggggctga atcgccctgg gggggcgcac
    15241 aactgacatt tatcgcccgc cgcgatgtta cgccacagcc accacaaacg ccgcaagcct
    15301 tactggagtt tgctaaagcc aatcccggca cggttaccta tccgcgccca ccggacttta
    15361 ccggcacggc gtttcttgaa cagttgctga ttatgctgac gcccgatccc gccgcattaa
    15421 aagaagcgcc ggacgatgcg actttcgccc gtgtcactgc tcccttgtgg caatatcttg
    15481 atgcgctgca tccgtatttg tggcgcgaag gaaaggattt cccgccttcg cccgcgcgga
    15541 tggatgctct gctgaaatcc ggcacattgc gcctgtcgct gacctttaac cccgcgcatg
    15601 cgcagcaaaa aatcgccagc ggcgatttgc ctgcaagcag ttacagtttt ggctttcgcg
    15661 aggggatgat tggcaacgtg catttcgtca ccactcctgc caacgcgaat gccagtgctg
    15721 cggcgaaggt agttgccaat ttcttgctct cacccgatgc gcaattgcgt aaagcagatc
    15781 ccgctgtctg gggcgatcct tctgttctcg atccgcaaaa actgcctgat gggcagcgcg
    15841 aaacattgca atcaagaatg cctcaagatc tgccgccagt actggctgaa ccgcacgcag
    15901 gttgggtaaa tgcgctggaa caagaatggc tacaccgtta cggtacgcat taatcttttt
    15961 gctgtgggcg atggtggcgg tgatttatgc accgctgatc ccggcagctc tcacgctgat
    16021 atcgcctgct ttgtcgttga cacactggca ggcgttattt gccgatccac agttaccgca
    16081 ggcattactg gcaacgctgg tgtcgacaac catcgcggcg gtcggggcgt tgttgattgc
    16141 cctgctggtg attgtggcgc tgtggcctgg gccgaaatgg cagcgtatgt gcacccgtct
    16201 gccgtggctg ctcgccattc cccatgtggc ttttgccacc agcgcccttc tgctctttgc
    16261 tgacggaggg ctgctttatg actatttccc gtatttcact ccgccaatgg acaaattggg
    16321 tattgggctg ggcttcacgc tggcggtgaa agaaagcgca tttctgctgt ggatcttagc
    16381 agcggtattg agtgaaaaac ggctgttgca gcaggtcatt gtgctggatt cgctgggcta
    16441 cagccgcagg caatgcctga actggcagct gttgccctcc gtcgcgcctg cgctggcaat
    16501 ggcgatgctg gcgattgtgg cctggtcgct gtcggtcgta gatgtggcga ttattcttgg
    16561 gccaggtaat ccaccgacgc tggcagtaat tagctggcag tggttaactc agggcgacgc
    16621 caatcaacaa acgaaaggcg cactcgccag cctgctgctg atgctgttac tcgccgccta
    16681 cgttttgctg ggctatctgc tatggtgtac ctggcggcgt actattcccc gcgtagatgg
    16741 cgttcgtaag cctgccacgc ctttattgcc aggcattacg ctggcgagtt ttttaccctt
    16801 gaccggtgtg ctgtgtgtgg ttctgctggc gatcctcgcg gatcagtcga cgatcaatag
    16861 tgaagcgctc atcaacagcc tgacaatggg gctggtggcg acattcatcg ctttgctcct
    16921 gttactgctg tggctggaat gggggccaca gcgtcgccag ttgtggctat ggttgcccat
    16981 tttattaccg gcactgcagc tggtggcggg ccagtacacg ctggcgctat ggctgaacat
    17041 ggatggaagc tggacggcgg tggtctgggg gcatctgctg tgggtaatgc cgtggatgct
    17101 gtttatcctg caacccgcct ggcagcgcat tgattcacgg ttaattttga ttgcacaaac
    17161 actgggctgg tcgcgggcca aaatcttctt ttacgtgaaa tgcccactca tgttgcgccc
    17221 tacgctgatt gccttcgcgg tgggattttc agtcagtatt gcgcagtata tgccgacgct
    17281 gtggctgggc gcggggcgtt ttccgacgct caccactgag gcggtggcat taagcagcgg
    17341 cggcagcaac ggtattctcg ccgcccaggc tttatggcaa ctgctattac cgcttattat
    17401 ttttgccctg accgcgttag tcgcaaaatg ggtaggttat gtcagacaag gactccgcta
    17461 atgctctgcg tgaaaaatgt ttcgctacgt ttaccagaaa gttgcttgct gacaaacgtt
    17521 aactttacgg tggataaagg tgacattgtc acgttaatgg ggccgtctgg ctgtggaaaa
    17581 tccactctgt tttcatggat gattggtgca ctggctggac agttttcttg tacaggtgag
    17641 ctatggctca atgagcaacg gattgacatg ctacccaccg cacagcgtca gattggcatt
    17701 ctttttcagg atgcactgtt atttgaccag ttcagtgtcg ggcaaaattt actgctggcg
    17761 ctaccggcga cacttaaggg gaatgcccga cgtaatgccg tgaatgatgc acttgagcgt
    17821 tcaggccttg acggagcttt ccatcaggat cctgccactt tgtctggcgg tcagcgagcg
    17881 cgcgttgctc tgcttcgcgc ccttctcgct cagccaaaag cgttgctact ggatgagcca
    17941 ttcagccgtc ttgatgtggc tctgcgcgat aattttcgcc agtgggtgtt cagcgaagtt
    18001 cgcgaactgg cgatccccgt cgttcaggta acgcacgatc tccaggatgt tcctgctgat
    18061 agttctgttc tggatatggc gcagtggtca gaaaattaca acaaactgcg ataacgcaaa
    18121 gtttttctca atgcgtcagt tcagaatggc gcactcaaaa ctacaatgtc gggattttcg
    18181 atgaaacgtg tttctcaaat gaccgcgctg gcaatggctt tagggctggc ttgcgcttct
    18241 tcgtgggccg ctgaactggc gaagcctctt acacttgacc agcttcaaca acaaaatggc
    18301 aaagcgatag atactcgccc cagcgcgttt tataacggct ggccacaaac cttaaatggc
    18361 ccttctggtc atgaacctgc cgccttaaac ctctctgcca gctggcttga caaaatgagc
    18421 accgaacagc tcaacgcgtg gatcaagcaa cataacctga aagccgatgc cccggtggcg
    18481 ctgtacggta atgacaaaga tgtcgacgcc gtcaaaacgc gactgcaaaa agcaggtttt
    18541 acgcatatct ccattctgag tgacgcgcta agcgaacctt cccgtctaca aaaactgccg
    18601 cactttgagc agctggttta tccgcaatgg ctacacgacc tgcaacaagg taaagaggtt
    18661 acggcgaaac ctgccggtga ctggaaagtc attgaagcgg cctggggcgc tcctaagctt
    18721 ttccttatca gccatattcc cggcgctgac tacatcgata ccaacgaagt ggaaagtgaa
    18781 ccgctgtgga acaaagtttc tgatgaacaa ctaaaagcga tgctggcaaa acacggcatt
    18841 cgccatgaca ccacggtcat tctttacggg cgagacgttt acgccgcagc acgtgtggcg
    18901 cagattatgc tctacgctgg cgtgaaagat gtgcgcctgc tggacggcgg ctggcaaacc
    18961 tggtccgacg cgggactgcc tgttgagcgc ggaacgccac cgaaagtgaa agcagaaccg
    19021 gatttcggcg tgaagatccc ggcgcaaccg cagctgatgc ttgatatgga tcaggcccgt
    19081 gggctgctgc atcgccagga tgcatcgctg gtgagcattc gttcgtggcc agaatttatc
    19141 ggtacgacca gcggttacag ctatattaaa ccaaaaggtg aaatagccgg agcacgttgg
    19201 ggacacgctg gtagcgactc gacgcatatg gaagatttcc ataacccgga tggcacaatg
    19261 cgtagcgccg atgatattgc cgctatgtgg aaagcatgga atatcaaacc agatcagcaa
    19321 gtttcattct actgcggcac cggctggcgc gcgtccgaaa cctttatgta cgcacgagca
    19381 atgggctgga ataacgtctc cgtttacgac ggcggctggt acgaatggag cagcgatcca
    19441 aaaaatccgg tagcaaccgg tgaacgcggc ccggacagca gcaaataaca ttgcaattac
    19501 tgacgctgga gcgatttcag cgtcagatac ccactccaca cccgcgtaaa ggtcgtcatc
    19561 cagcacaacg caccaaatac ccacgcaaac cacggaaaac aggccggaaa gaggcaactc
    19621 agaacaaaca gcaaaatcgt ctcgctgcct tcggttaacc cgcccagata ataaaacgat
    19681 ttatgcgcat aaccgggatt atcaatctga tgtttcgccg ccagcgcagc aaaagcgaga
    19741 aaactgctgc ccgtgccgat aaacgcaaac agcagccagc cgcctgccag ggcgttttgc
    19801 tccggtgcgg cgaggataaa cccaaaaggc accagcgcgt aaaaaagaaa atcgagagaa
    19861 atatcgagaa agccgcccgc gtcagtaagc cctctgcgcc gcgccagtgc gccatccaga
    19921 ccatcaagca gacgattcaa caaaatgacg actagcgccg ccagatacca gcccagagcc
    19981 agaaacggca gagccagcac gccgatggca aatcccacta atgttaaacc atctggcgta
    20041 atgcccggtt tatcaagaac ccgcacgcac tgatgcaaca acggtttaat ccggggatga
    20101 agatggcggt ctagcacagg cactccttaa atataaagcc tttctgattg agcaacagtg
    20161 cggatattat ggcatttttc gcttatctgc ccgtgtgtaa tttatgaaaa tgattgaagt
    20221 tgttgccgcc atcattgaac gtgatggcaa aattttactc gcgcaacgcc ccgcccagag
    20281 cgatcaggcg ggattatggg agtttgccgg tggtaaagtc gagctggatg aaagccagca
    20341 gcaggcgctg gtgcgtgagt taaacgaaga attgggcatc gaagcaactg tgggtgagta
    20401 tgttgccagc catcagcgag aaatttcggg gcggattatc catcttcatg cctggcacgt
    20461 acccgacttc cacgggacgt tacaggcaca tgaacatcag gcgctggtct ggtgctcacc
    20521 tgaagaggcg ctgcaatatc cgctggcccc tgctgacatt ccactattag aggcatttat
    20581 ggctttacgc gctgccagac cagcggatta gtgttaaggg ttttgtcatc acgctggcat
    20641 tgcagcagta ttccctcggc tttaattacc gctccttcag aataattttt atcctgataa
    20701 acacagcact gagtacaggg ctgcgctgac tgcccgcctg aactgaacac ttctggcggt
    20761 acgtttacct ccacgtccgg acgataatgc gggttagcca gtgcaattaa tggaaatgct
    20821 aatactacgg cgaacaatgc tcgactcaca gggaactcct taacgttatt gtctctgcta
    20881 ctgataacgg tagccgggtg gcaaaacttt agcgtctgag ttatcgcatt tggttatgag
    20941 attactctcg ttattaattt gctttcctgg gtcatttttt tcttgcttac cgtcacattc
    21001 ttgatggtat agtcgaaaac tgcaaaagca catgacataa acaacataag cacaatcgta
    21061 ttaatatata agggttttat atctatggat cagacatatt ctctggagtc attcctcaac
    21121 catgtccaaa agcgcgaccc gaatcaaacc gagttcgcgc aagccgttcg tgaagtaatg
    21181 accacactct ggccttttct tgaacaaaat ccaaaatatc gccagatgtc attactggag
    21241 cgtctggttg aaccggagcg cgtgatccag tttcgcgtgg tatgggttga tgatcgcaat
    21301 caggtacagg tcaaccgtgc atggcgtgtg cagttcagct ctgccatcgg cccgtacaaa
    21361 ggcggtatgc gcttccatcc gtcagttaac ctttccattc tcaaattcct cggctttgaa
    21421 caaaccttca aaaatgccct gactactctg ccgatgggcg gtggtaaagg cggcagcgat
    21481 ttcgatccga aaggaaaaag cgaaggtgaa gtgatgcgtt tttgccaggc gctgatgact
    21541 gaactgtatc gccacctggg cgcggatacc gacgttccgg caggtgatat cggggttggt
    21601 ggtcgtgaag tcggctttat ggcggggatg atgaaaaagc tctccaacaa taccgcctgc
    21661 gtcttcaccg gtaagggcct ttcatttggc ggcagtctta ttcgcccgga agctaccggc
    21721 tacggtctgg tttatttcac agaagcaatg ctaaaacgcc acggtatggg ttttgaaggg
    21781 atgcgcgttt ccgtttctgg ctccggcaac gtcgcccagt acgctatcga aaaagcgatg
    21841 gaatttggtg ctcgtgtgat cactgcgtca gactccagcg gcactgtagt tgatgaaagc
    21901 ggattcacga aagagaaact ggcacgtctt atcgaaatca aagccagccg cgatggtcga
    21961 gtggcagatt acgccaaaga atttggtctg gtctatctcg aaggccaaca gccgtggtct
    22021 gtaccggttg atatcgccct gccttgcgct acccagaacg aactggatgt tgacgccgcg
    22081 catcagctta tcgccaatgg cgttaaagcc gtcgccgaag gggcaaatat gccgaccacc
    22141 atcgaagcga ctgaactgtt ccagcaggca ggcgtactgt ttgcaccggg taaagcggct
    22201 aatgctggcg gtgtagcaac gtcaggcctg gaaatggcac aaaacgctgc gcgcctgggc
    22261 tggaaagccg agaaagttga cgcacgtttg catcacatca tgctggatat ccaccatgcc
    22321 tgtgttgagc atggtgatga aggtgagcaa accaactacg tgcagggcgc gaacattgcc
    22381 ggttttgtga aggttgccga tgcgatgctg gcgcagggtg tgatttaagt tgtaaatgcc
    22441 tgatggcgct acgcttatca ggcctacaaa tgggcacaat tcattgcagt tacgctctaa
    22501 tgtaggccgg gcaagcgcag cgcccccggc aaaatttcag gcgtttatga gtatttaacg
    22561 gatgatgctc cccagggaac atttcttatg ggccaacggc atttcttact gtagtgctcc
    22621 caaaactgct tgtcgtaacg ataacacgct tcaagttcag catccgttaa ctttctgcga
    22681 tagcagcaga tatgccagta aagaaatccc attagactat ttttttgatc atcttcttcg
    22741 ctttcgaaca actcgtgcgc ctttcgagaa gcaagcatta tataatgcca ggccagttct
    22801 tcttcaattg tcccgttttg aaaagctgtg cttgatatcg agatcatcca tgataattcc
    22861 gccgcccata ttagcttcgc cgaggattta ccggagcgat tattagcgca atcagagata
    22921 taatctgagg gaaaaacagc aaatttattc aacaaggcga taacctgctc tggggcttcc
    22981 tccatgtttg ctttaaaggt attggctcca tggtcgccag aaagaaaatg ctccattaag
    23041 gcacaataac tttcgctatc ttcgataccc cattgatcct ctaaagactc gcgtctttta
    23101 ctaatgatat tgatcgagtc aaaaggaagc acatgatatt ggaaggtatc tttgccaggt
    23161 tcaggctttc gcggccagaa ctccagcgtt tcagaccatt gcttatgata gaatcgataa
    23221 ggtgcgatca attgtagcgc ctgtaacttc tcgatactga gcggctcaat acctttagcc
    23281 tgataataat gcagttgttc tttttttgct ttaaaaccgg cccgaacaat aagccccatc
    23341 ataattaata gataaagaaa agagcatccc gcagtaatca ggcctctttc attcaaaccg
    23401 ttggatgtta tcgctgcgaa tacaaacatt acagcgacaa cacatgttaa ataacctccc
    23461 cacttacaaa gcagcatggc cttattttct ttaatcatcc gttcaaaatt actattaaat
    23521 atttcccagc cattaaaaga atacttctcg ctcccaggat ggttttgtaa taaaactttt
    23581 ttcatcacat tccctgttat tacatacttt agaggcgata attttatcca tgcaaaaaaa
    23641 tatccaacga attttcttga tccgctggat atttcagaat atgactcgaa tagcacgaaa
    23701 gattcgctcg cttacgctat cgccccgctt ccggcttcat ctgctggcgg actttttttc
    23761 gcactacgtt tacgcggtgc agcctttttc ttatcagcac tgccaccact gcccggagcc
    23821 acaatgccgc gaaactgccg caccggagta cgtttggctt gatcaataag ctgatatagc
    23881 gtccccacca gcggctgcat aaagtcctga tagcgacact gcttttcgct gatttgcgtc
    23941 agcactgatt cccagtgcgc ggtcatgtcc ggtcgcgtag ccatctccgg cagcgaatgg
    24001 aatagcgctt ttccggcgtc ggtagagtgg atatagcgcc cttttttggt caggaaacca
    24061 cgcttgaaca acagttcaat aatcccggca cgcgttgctt ctgtccccag gccatcggtc
    24121 gcacgaagga tctttttcag atctttgtcc tgcacaaagc gcgcgatccc ggtcatcgcc
    24181 gaaagcagtg ttgcatcggt aaaatggcga ggcggctggg tttgccgctc taccacttca
    24241 cctttttcac acagcaactc atcgcctttc gccaccacag gcagtggcgt gccgtcgttt
    24301 tcttcatcgc gctctttgct gcctaacagc gcgcgccaac ctgcttcagc aagaaaacgc
    24361 gctttagcga caaatttgcc tttggcaatg tccagttcga taacacactt gcggaacacc
    24421 gcatccgggc agaactgcat caagtactga cgggcaataa ggttatagac cttcgcttcg
    24481 ttctccgtca ggttgatcgc agaactccgt gcggtcggga tgatggcgtg gtgcgcatcg
    24541 acctttttgt catcccaaca gcggttgcgt atatctggat ctaccactgg ctgcggcaac
    24601 agatctggtg catgaacgct gatggcattc atcaccgcgt ggcgtccggc aaaatgttct
    24661 tctggcaaat agcgacaatc ggaacgcgga taagtgatta gcttgtgcgt ttcgtacagc
    24721 ttctggcaga tatcaagcac gttctgcgcg ctcagaccaa agcgttttgc cgcttcaatc
    24781 tgcaacgccg aaagcgaaaa cggcagcggc gcggattctg attcccgttt atcgttatag
    24841 ctggtgacaa tagccggttg accactaatg cggttaacca catgctccgc cagtggacga
    24901 tgtaacaagc gcccttcttc atcctggtac ggttcacacg cttcgctcgg ttgccagata
    24961 gcggtaaacc gctcatcggc aggtgtcacg atatgcgctt tgacttcaaa gaagtctttc
    25021 gccacgaagt tttcaatctc ttcatcgcgg cgcaccacca gcccgagcac gggtgtctgc
    25081 acgcgtccca cggaaagtac gccctgataa ccggcattgc gaccgagaat ggtatacgca
    25141 cgggtcatat tgatgccgta cagccagtcg gcacgcgctc gcgccagcgc agaaacgcac
    25201 aatggcacaa actcactgtt ggaacgaaga cggtcgatcg cccgctcaac cgcctgcggg
    25261 ttcaggtcgt ttatcaagca acgctgcacc tgctggcgct tttccggtgc cagttgcaga
    25321 tagtccagca cttcatccac cagcaattgc ccttcacgat ccgggtcccc ggcgtgaacg
    25381 atttcgctgg cttcatgcag gaagcgtttg atgacgttaa gttgtttggt cacggagggt
    25441 cggggctgta attgccactt ttccgggaca atcggcaaat ccgcaagatt ccagcgcgca
    25501 tagcggctgt cgtaggcgtc tggctgcgcc tgctcaagca ggtgaccgat acaccaggtc
    25561 accacctgac cattaccgca ctcgataaag ccatcgcctt tacggtgcgg tttgggcagg
    25621 acatcagcaa tggcgcgcgc cagactcggt ttttcggcaa taaacaaccg cattgaatta
    25681 acggatctca accatggcac gaccgccgcg cgctggcacc agttcgccaa tcgccgtcag
    25741 ttcaatgcca aactcggcgg ctgtagcttt aacctcattt tctgcttccg gcatgaccgc
    25801 cagcagcaaa ccgccagaag tttgcggatc gcacagcaaa tcgcgcactt cacgcggcat
    25861 ttcacccatc agatgaccgt agctggcaaa gttacgttcg gtgccgccag gcactgcgcc
    25921 caacttaatg tactcttcaa cccccggcaa ttttgggatc gcgtcatagt caacgcgcgc
    25981 ctgcacacct gcgccctgac acatttcgct caaatggccc agcagaccaa aacccgtaac
    26041 gtcggtcatc gcttttacgc cttcgatgtt ggcaaaggac gcgcctgcga tgttcatccg
    26101 gcacatcact tccgtcgcca gtccctgatg ttctggtttc agcagtgatt ttttctcagc
    26161 cgtggtaaga acgccaatcc ccagcggttt cgtcaggaac agtttacatc cggcttgtgc
    26221 ggtactgttt ttcttcaccc gctcggtcgg tacgatcccc gtcaccgcca gaccaaaaat
    26281 cggctccggc gcatcgatgg agtgaccgcc agccagcgca atacccgcct gacgacatgc
    26341 atagcgtcca ccttcggtca cttcgcgggc aatttccggg gaaagtttat taatcggcca
    26401 gccgagaatc gcaatcgcca taatcggctt gccccccatc gcgaagatat cgctgatggc
    26461 gttagtcgcc gcaatgcggc caaaatcgaa aggattatca acgatcggca taaagaagtc
    26521 ggtggtactg ataacgctgg tgccattgcc cagatcgtac accgccgcat cgtcgcgggt
    26581 ttcattaccc acaagcaaat tcggatcaac aaacttcgcc tgctcactgt gcaggatggt
    26641 ttccaacact tttggggaaa ttttacagcc gcaaccagct ccgtggctgt attgggtcaa
    26701 acgaatcgag ttctcgctca tggacatctc ctgtcaatgc aatccgggta tggtaaccct
    26761 cattccgtga agtgataagt gagagtgtct gaattcctgc gcctttgctc acaatccaga
    26821 cagtttcgcg acaattatca gaaataagtc acaaacggcg tcgggtccgg gacgtttatc
    26881 gacgtagatg ctttcaactg cggcgtaccg aggtagagaa aaccgacaat tttatcctgc
    26941 tcacggcaac cgaatgcttc acgcactacc gggctttcag ttaatgcgcc actgcgccag
    27001 atgccgccaa acccctgggc aacggctgcc atttgcatcg ccatgaccgc gcatccggca
    27061 gacatttcct gttcccatcg cgggacttta tgattttctt cgcatttcgc caccaccgtg
    27121 atgatgagcg gtgcgcggaa tggcgcatta cgggctttgt cgatagcttt gtcatcacta
    27181 ccggcagcaa tcgccccctg ttccagtacg gcgctgaaac gctcgcgccc ttccccttca
    27241 atcacaaaaa aatgccacgg ttgcatggac ttatggtctg gcgcgcgcat acccgcacgc
    27301 aggatgtttt gcagttgttc acccgttggt gcgggttcag ccaggcggga ggcgctacgg
    27361 cgattgatca atagttcgag tgcatccatt tgattaactc ctgtcgtgat atttattcac
    27421 aaaattaaca cgagagtgga ttttgttaca gcacagtccg caattcctgc tgacaagtgc
    27481 cggttgggtc attacgataa ccacatctat tgcgcctgtg acaggtgtga ccttaagttg
    27541 ggagaataca tgcgaaccct ttggcgattt attgccggat tttttaaatg gacgtggcgt
    27601 ctgctgaatt tcgtccgtga aatggtactt aacctgttct ttatttttct cgtgctggtt
    27661 ggtgtgggga tctggatgca ggtcagtggt ggtgattcga aagaaacggc cagtcgtggc
    27721 gcactgctgc tggacatttc tggtgtgatc gtcgataaac ccgacagttc tcagcggttt
    27781 agtaaattaa gccgccagct gcttggtgcc agttccgatc gtctgcagga aaactcactg
    27841 tttgatatcg tcaacaccat tcgccaggcg aaggacgacc gcaatatcac cggtatcgtg
    27901 atggatctga aaaacttcgc aggcggcgac cagccgtcta tgcagtacat cggcaaagcc
    27961 ctgaaagagt ttcgtgacag cgggaaaccg gtttatgccg ttggcgagaa ctacagccag
    28021 gggcaatatt atctcgccag tttcgccaat aaaatttggc tgtcaccgca aggcgtggtt
    28081 gatctgcacg gttttgctac caacggtctg tactacaaat cgttgctgga taagctgaaa
    28141 gtttccaccc atgtgttccg tgtgggtacg tataaatctg ccgttgaacc atttattcgt
    28201 gatgatatgt caccggcagc ccgcgaagct gacagccgct ggattggtga gctgtggcaa
    28261 aactatctga atactgttgc cgctaaccgg cagatccctg ctcagcaggt attccctggc
    28321 gcgcaagggt tgcttgaggg tttaaccaaa accggtggcg ataccgcgaa atatgcactg
    28381 gaaaacaagc tggtcgatgc actggcatcg agtgccgaaa tcgaaaaagc tctgaccaaa
    28441 gagttcggct ggagtaagac tgataaaaat tatcgcgcca tcagttatta cgattacgca
    28501 ttgaaaacgc cggcagatac cggcgacagc atcggtgtcg tcttcgctaa tggcgcaatt
    28561 atggatggcg aggaaactca ggggaatgtt ggcggtgata ccactgcggc acaaatccgc
    28621 gacgctcgcc ttgacccgaa agtgaaagcg attgttctgc gtgttaatag cccaggcggc
    28681 agcgttaccg cgtctgaagt gattcgcgct gaactggcag cagcccgggc tgcgggtaag
    28741 cctgtggttg tatcgatggg cggcatggcg gcatctggtg gttactggat ttccacgcca
    28801 gctaattaca ttgtggctaa ccccagcacc ctgaccggtt ctatcggtat cttcggcgtg
    28861 atcaccaccg tagaaaatag tctggattcg attggtgttc atactgatgg tgtctcaact
    28921 tcaccgctgg cagatgtttc tatcaccagg gcactgcctc cggaagcgca gcagatgatg
    28981 cagttaagca ttgagaatgg ctataaacgc tttatcacgc tggttgctga tgcgcgtcat
    29041 tcgacgccgg agcagattga taaaatcgcc cagggccacg tctggaccgg tcaggatgca
    29101 aaagctaatg ggctggtcga tagtctcggg gatttcgatg atgcggtcgc caaagcagca
    29161 gagctggcaa aagtgaaaca gtggcatctg gaatattacg ttgatgaacc gaccttcttc
    29221 gacaaagtga tggacaacat gtctggttct gtccgggcaa tgttgccaga tgcgttccag
    29281 gccatgttac ctgcaccgct tgcctcggta gcctctatcg ttaaaagtga aagtgacaag
    29341 ctggccgcgt ttaacgaccc acaaaaccgt tatgcgtttt gcctgacctg cgccaacgtg
    29401 cgttaagtct tgtactgagt ggccgacaga tcgtcggcca tatttttaat cctcttcccg
    29461 ctgtctcgcc ccatatttcc ttatccgcat agtatcaggt gcgctccccc tgcctcacgt
    29521 atatactttt gctctttcga tatcattcat atcaatatca tgcaaaagaa atcaatttac
    29581 gttgcctaca cgggcgggac catcgggatg cagcgttccg agcagggtta tataccggtg
    29641 tcaggtcatc tacaacgcca actggcgctg atgccggaat tccatcgccc ggagatgcca
    29701 gatttcacca ttcatgaata tacgccgctg atggattctt cagatatgac gccggaagac
    29761 tggcagcata ttgctgaaga tattaaagcg cactatgacg actatgatgg ttttgtcatt
    29821 ctgcacggca ccgatacgat ggcatatacc gcctctgcgc tgtcgttcat gctcgaaaat
    29881 ctcggtaaac cggtcattgt gacagggtca caaatcccgc tggctgagtt acgctctgac
    29941 ggacaaatta atctgctgaa tgcgttgtac gttgcggcga attatccgat caacgaagta
    30001 acgctctttt tcaataaccg attgtatcgc ggcaaccgca ctaccaaagc ccatgccgat
    30061 ggttttgatg cgtttgcctc tccaaacctt cctccgttac tggaagcagg tatccatatt
    30121 cgtcgtttga atacgccacc cgccccgcac ggtgaagggg aattaatcgt tcatccaatc
    30181 accccacaac caattggcgt agtgacgatt tatccaggga tttctgctga cgtcgtgcgc
    30241 aactttctgc gccaaccggt gaaagcattg attctgcgct cctatggcgt gggtaatgcg
    30301 ccacaaaaca aagccttcct gcaggaatta caagaagcca gcgatcgcgg tattgtggtg
    30361 gtcaacctga cacaatgtat gtccggtaaa gtgaatatgg gtggttatgc caccggtaac
    30421 gccctcgccc atgctggcgt tattggcggt gcagatatga ctgtagaagc cacgctaacc
    30481 aaactgcatt acctgcttag ccaggaactg gatactgaaa ccattcgcaa ggccatgagc
    30541 caaaacctgc gcggcgaact gacgccggat gattaaggag actgcaatgc cccctcgcgc
    30601 cctgttactg gtcgatttac aaaatgattt ctgtgctggt ggcgcgctcg ccgtgccgga
    30661 aggtgacagt acggtggatg tcgctaaccg cctgattgac tggtgccagt cgcgcggtga
    30721 agcggttatc gccagtcagg actggcaccc ggcgaatcac ggcagttttg ccagtcagca
    30781 cggtgtagag ccttatacgc caggccaact cgacggtttg ccacaaacct tctggccaga
    30841 tcactgtgtg cagaacagtg aaggcgcaca attacatccg ttactgaacc aaaaagcgat
    30901 cgcagcggtg ttccataaag gcgaaaatcc tttagttgac agttacagtg ccttttttga
    30961 taacggccgt cggcagaaaa cctctctcga tgactggtta cgcgatcatg aaatcgatga
    31021 attgatcgtt atgggcctgg ctactgacta ttgcgtgaag tttaccgtgc tggacgcgtt
    31081 acagttaggt tataaggtaa acgtgattac cgatggttgt cgtggcgtga atatccagcc
    31141 ccaggacagt gcgcacgcgt ttatggagat gtcagcagct ggggcaacgc tatatacgct
    31201 ggcagactgg gaagagacgc aggggtaatt ttacgctggc ctacaattct gtactggcat
    31261 tgtaggccaa ataaaacacg tcagtggcac atctggcaat tgatgccatc aacgaaagat
    31321 ttaattcacc tcagaaatct cttccaacga caccttccgc gtttcgatgc caaaaatgga
    31381 gagaaccagc gcacagagca ctaacatgac accaagtacc ataaacaccg tgatcgaccc
    31441 ataatgtgtt aataatgccg caacgccata gggagtgaag actgcgacga tccgtccgac
    31501 ggcattaacg aaacccgaac cgcgcaggcg taaatgcgtt ggccaaagct ccgggatata
    31561 aaccgccgac gcgaagcaaa catacatgta taaaaagaag atcatcacca ggccatagat
    31621 taaaatcgcc cactctgtag tctgaattga atagatatag cctaacacgg caataataat
    31681 aagtaaggtg gagccaaata accgacgagg aaaatgatca ataattaatg ccgcaataaa
    31741 tattcctacc ggagcgccaa tcataataac agcggtcatt aatattgatt tatcgacatc
    31801 aatgccggag ttaacaaata tggtcggtat ccataccgtg atggtataaa gcgaaatgtt
    31861 catcgcaatt aaaacagtaa tcgcgactaa tgtacgtcgt aacatttcac ctttaaacag
    31921 gagccagaaa gtacccttga ttactttaac tttgctgttg ctctgatacg aagtaagcgg
    31981 gggtaaacga atactcttct ctctttcaat ttgctgctct acttcacgaa gttggcattc
    32041 tgcacctgcg atttgccctt tccctgccag ccatcgtggc gactcaataa agtatttacc
    32101 tgagagaagc caggctaaca gtatgccaat accacccaac agaaacatta tccgccaact
    32161 aaaaaaagcg ataaccacca cacctatcgc cgcagacagc atgggcgacc agttaccaac
    32221 aaatgagagc cgcgcggacc attttccacg caccgtcgcg gggataaact ccgtaaatga
    32281 ggcatagcca accataatca gtgcccccat tcctgttccc atcaggaagc gaaagaagat
    32341 gagccagtac atatcaggga caaaagcggc ccctgttgca gcaataccga cgatgagaag
    32401 atttatgcga aacgccctgc gccgcccaaa gtaatcaccg atgaacccac cggtaagaga
    32461 gccgatgaaa taaccaaaca ttaatgccga ggtaaaggcg gcattgagga aattatttga
    32521 ccatccattg cttaccagct ttgctaagac gacattaccg gagtaactca aaaaccctgt
    32581 taataacaga ctaaagctta taataccaaa tatacgataa tgaaaccggg ccaaaggcaa
    32641 gcggtccagt cttgcgccaa tttgatcata ttgttccatt gggtgcctcg gatttgctaa
    32701 aaaaggtagc aattgcggca aattcacttg ccgcatcttt atcatcattt tatttccagg
    32761 cacaggaaca aataatattg acaacgtgtg ctaaaattac cacaccagct ggatattatt
    32821 gtctttgcaa aatgctatcc actctgcacc cggtgattta tcagttatta tgtaattaat
    32881 atgactaaag tcagctaact ggacaaaagc tttgcgatca aacttagagt gatcaaccaa
    32941 taacgcaact tctgtcgcct gacggatcat tgtttttttg atttcagctt ccgcttcatt
    33001 agagtccagc gcgccgctgt taatatcaag acctttacaa ctcattacca tgatatcgac
    33061 atgatagcgg ctgatgatct ctttagtaat tcttccttgc agtgaaagtg tatttttgtt
    33121 tagttcccca cccgttgaaa cgactttaat ttctgactga gccagaacat gaatcgcttc
    33181 cgccgagttg gttagcaacg tcaggtcact acggtcctgt aacaatttga gcaattccat
    33241 tacggtacta ctggaatcgg ctgccatggt ggttttattg tcgataaagg gtagtgcctt
    33301 gcgtgcaata agctgcttct cttcataaaa cgatgaagcg cgcttataaa aatggatatt
    33361 ctccgtcaac atcgctgtat ttaaaacagc accaccatag gttctggtca aaaagccttc
    33421 atcttccagc ttctcaagat cgcggcgaat ggtttcttcg gttacctgaa aaatcccact
    33481 caaatttgag actgtcacct ttttatcgtt ggcaaccatt tgcttaattg cctgaatcct
    33541 gtcttttgcc gccacgatta cacccctgta tctttttaca tcacattagc gcgattatcg
    33601 cataaccgat gtttactttc aaaataacct gtttgaatca cagattttca tcacagtttt
    33661 cacagaaaca gaggtgaatc gtgttgagta tttaacgctc cagggcctct gccatttccc
    33721 tcattaatgt tgcgtctgca tccgataagt tgatattcag tgccgcgaca ttttcgcgta
    33781 cctgttccgg tgcagtagcc ccactaagaa tggagattaa atcactctgt tttaatatcc
    33841 acgccagtgc cagagtggga attgtgcact gataacgagc acaaagtggc tgccactgtt
    33901 caagcatatc aatcactttc agcatgtttt cacgctggaa ccagacttta tttgcccgag
    33961 cgccgcccgg aacgtaatca cgagtgatgg tgccggtcaa caatccctgc tctagcgggg
    34021 aataaacctg aaccacaatg ccattatcac gacatagcgg cagcagttcg ttttccattg
    34081 cccggtcgag gatactgtat ttcgcctgaa taatatccag ttcaccatat tgcagatact
    34141 cgcggatatg gtcagcatcg acgttagcag cgcctatagc gtgaattttc ccttcagcct
    34201 ttaactcatt aagcactgcg acagtttcag cgatcggcgt ataaaatggc ggcaccgact
    34261 gccagtgcgt catgtagata tcgatgtaat caatacccag acgttgcaag ctggcttcta
    34321 cctcttcgcg gatagattcc ggggaaaggt ttttatacaa ctgccgatcg ccaactttgt
    34381 tgaataaact tccttttcgt tcccagacaa tgccgcattt ggtttctact acaacctgtt
    34441 cacggggcag tttttttaac gcctgaccga cgataacttc actattgcca aagttatatc
    34501 ctggtgcagt atcaatcaga ttaatgccgc aacgatgggc ttcaagaatc gtatcaatac
    34561 atatttgccg atcgagatcg ccattccatg caggaccgcc gccaatggcc catgtcccca
    34621 accccattcg cgaaagcgta atatccgttg tgcctaaagg tatctttttc atttgccttc
    34681 attatccttc gtattcttcc agcaattgtt cgaccagctt tctgtttttt acgccggtgg
    34741 tggcaccgac gcttagaacc gagatagccg ccgttgcatt ggcaaagcgt gcgcattcac
    34801 gcagattttt gccttctaac agtgccgcaa taaaacctga agcaaagtta tcgcccgcgc
    34861 caatggtgtc gatggcggtt attcctgcga ccgccggcac cttcatcgtc atgtcaccac
    34921 gcttgataaa gcagccgtct ttacccgttt taatcaccac cgtttttacg ccgcacgcaa
    34981 gaaagcagtc agcaatttca tccagtgtct ctttcccggt gagtaatttt gcctcggcat
    35041 aattaggaaa cagataatcg acataactca atgcttcgca aatatcatcc agcgtttcat
    35101 tcaaccgcgg tttaatcata tcggcacaga taatcatctg ccgggcttta gcttgcgtaa
    35161 aaatttctgt tagcgcttta ccatccaata gtggactgtt gaaaatactg gccagtgata
    35221 ataatttcgc ctgggaaaac cgagcaaaat caacatcgtc aatattcaat ttccacagac
    35281 tgccgttacg gtttgtgaca aacgtccgct cgccatcctc cgtcaccagt ccaacgttaa
    35341 tagaggtatc tatgttgacg tcctgtttca ggctttgaat atcaatattc tctttgcggc
    35401 aatggtcgag aataaattgt cctgcggcat ctttaccaac acgactcatt aatgctgtac
    35461 gatggcccag acgagaaata attgttgctt catttattgc atcacccccg gtggtcattg
    35521 cgattctttc aagaggataa gaatccacat caaagatatt tttactgacc ggttgcaatg
    35581 gaatatcaac aatagcggca cctatacaaa taacgtcgag attatccata tcattattcc
    35641 gctttgccat cagaaccgaa cagtttaatt ttttccaatg cccgttcttt tacagcttta
    35701 cgtacttcac gttctaaatg caggaaaggc tgatcctggt tttctttcac tgcgaccatt
    35761 gcggcctgac agagctccgt atggatgttg attttcgcga tacctaacga aattgcagtt
    35821 ttaatgtcgg catcactgat ccccgatgcg ccatgcaaaa ccagcggaac agacacggca
    35881 tcgcgtacgc gtttgacgac ctcaaagttc aattgtggct ctgacgtata aaccccatgc
    35941 tggttgccga tggcgacagc caatgagtca cagcccgtac gttcaacaaa ttcagccgcc
    36001 tggtcaggat cggtatagtg atagcccgcc agagcttcct cataaacggt ttcattaccg
    36061 acatgcccta attccgcctc taccggaata cccagcggat ggaagaaatc aacagcctct
    36121 ttggttaaac gaatattttc ttcgaaatca aatgcggaag catcacgcat taatgaattc
    36181 ataccatgag tccaggcgtt atgaataatc tccatactcc gaccatgatc ccaatgagtt
    36241 attaccggca cagttgcttt tcgtgccatt gataccatca tgtgagagaa atcttcaaat
    36301 gaggtgttgc cgacaaaacc tgtaccaaaa gaaataataa ccggggattt cgcttcttcg
    36361 gctgcgtcga taacgcccat caacatttct gcattccata cgttaaaatg ggcaattgca
    36421 taatgtttat ttgtggcatc gttttcccaa tatctgatat ctgcgagcat gttctgttct
    36481 cccgtattaa ttaatcgcta atcttaataa cgcctttaat aatgtcgcgt ttattgttaa
    36541 ctgactcttc aaatgcctgt tgtacatccc gataatcgta aatatgcgtc accatcgatt
    36601 tcacatcgaa tcgccctgaa gaaatagctt caatcgtgac cggataacga ttggcgtagc
    36661 ggaataccgt ctggatagta acttcgcgat ttattttgag gaaattgatt gccgaatcgc
    36721 cgggtacagt accaacaatc ataattttac cgccgcgcat taccagataa ggtgcctgtt
    36781 taacggtgac cgcagaaccc gctgtttcga aaacaatatc tgcgcccatg tcttcggtaa
    36841 attgctgaca gcgtgcaata gtgtcttctt ttgcgccgtt aataaccact gtcgcaccaa
    36901 gctgttccgc cattgccaga cgtttttcca gcacatcaac gacggcaatt tccgttgctc
    36961 ccaggcattt gcacgcttgc aacgtcatca aaccaataca acctgatccc agaataatta
    37021 tcttcttacc cggtttaaca tctgccagca tcgcggcatg catcccgact gcggcaggct
    37081 ccaccagcgc cccttccatc gtgtccatat tgtcgggcag tttgtaagta aagctctccg
    37141 gatgacacag atagtgcgtt aatgcgccgc ggtagttggg ttgtgtcgcc ataaaatcaa
    37201 cgtccgggca gatgttatat ttgccttcca gacagtaacg acagtgaccg caaggaacgc
    37261 caggttcgat atttacccga tcccccggtt taaatttgcg cacgcggctt cccacagcca
    37321 caaccgtccc ggcgcattca tgacccaggc caatttcttg atttgggtct ttaggcggaa
    37381 taaacgggcc tgattcaaaa ccatgtacat ctgaaccaca aataccgaca tattctactt
    37441 taatcaaaac ttcatcttct ttaggcactg gtatttctgc tgaaataatt ttcattgtgc
    37501 ccggcacctg caatattgct tttgaatttt tcattttgaa tccacccgta ttatttattg
    37561 gctactgcat caatacttaa actttcaacg gagacacctt tggtttcgat tccaatggtg
    37621 gcgatagcaa ttgcgacaat aattgaaacc gcccccagaa gaataaagac tcccgtaacg
    37681 ccataactac tgagcagcac tgcaacggca taaggtgcgg caataccact gattcgccct
    37741 accgcattcg ccagaccgga gccacggagt ttggcctctg tcggccagat ctcagggaca
    37801 tacactgccg aggcatagca aacgtacata tagacaaaag taatcaggaa gaaaccaatc
    37861 aacgttatga gcaacatact ggtttgcagt gaatagatat atccgagcac cgcaatcagg
    37921 attaatagcc ccacacccat tgttttacgc ggaattttat ccatcaccag catggcaata
    37981 aaaatgccaa atggcgcgcc aaacatactc atggtattta aaacaatcga gtcttttaaa
    38041 ttaatcccct gtgtcatgaa tatcgttggc aaccagttaa ttagtgtgta ctgcacaacg
    38101 ttcatggcaa tcagcacaca agagcctaat atcacgcgtt tcagtaatac tcctgtcagt
    38161 aaggctgaat acggcaccgc ttgtggcgtt ttaccgtcat cagcaataac cacgggcggc
    38221 aaaggtttac ccgtctggtg tatgacgcct tcttctatac tccgcatgac tttctcggct
    38281 tcctgatacc gtccgcgcga ttccagccag cgcggggatt caggaaagta gcgccaggcc
    38341 agcgccgtag cgataagcga caatattgca gggataagca gttgtacccg ccagttccac
    38401 tctgcactaa tcagcggcgt gagtcccatc gctatcaatg aacagagcgg atatgaccag
    38461 ttgccaataa aggaaacccg acttgaccac gttccacgat ttctaccggg catatattcg
    38521 gtgaaaccag caaacagcgt aaccagtaaa gctcccagcc caactcccat cacaaaacgg
    38581 caggcaataa ggaaatccat attcggtgag aaagcaccga caaccatcga ggcaatatga
    38641 atggcctcat agaggatgaa cgcatttctg cgaccggttt tgtcgccaat gatgccgccc
    38701 accagcgcac cgagaaacat tccggcggtc gtgattgctg agaatgtggc tgtggtggaa
    38761 ttatctgtcc agcccaacgc tttcagctgc gcgaggatca agccaccaac ggcattactc
    38821 cagcagacaa gcaagccaaa cgcgacgatg gcaaacattg atgaatgcca gcggcaatcc
    38881 ggtaagcgat ccagccgtgc accacaatgc ggttttgtta tctgttccat gcgataacgt
    38941 tccttcagga gagtggttat tcatcaaagt cgtaagtcat gatcactttg attgcggttt
    39001 tatcgaccat cgcatcaaac ccttcgcgcc attgcgacag gccgatacga tgcgtaatca
    39061 tcggtttgac tttgatagcg ccgctggcta agagcctgat agcgttacgc catgaggtgg
    39121 agtcataggc catatgccca atgatgcttt tgttccaggc ggtaatgtca ttaatcgaga
    39181 aatcaagagg tttgaagccc attccaacgc gtaccacttc cccgttcggg cggagcattt
    39241 cgatggcttg tttcagtgcg atattggcac cggagcattc aatcaccagt cccagattgt
    39301 ctttgccaca aatttgctgg cagcgcgcca ccacatcttc ggtagaacca tttactactg
    39361 ccgtcgcacc cagttctttt gcaaccggga agcggaccgc cacatcttct tgcagaccaa
    39421 cgacgacgat atttaccgcc cccataattc gcgccatttg tacggagaac agcccgagtg
    39481 ggccagtgcc gatgacgacc acatcctgac caggaaggaa tttcgattgc tgcgcgatgg
    39541 atttgtaggc attacaaata gggtcaagta cggctgcgtc ctcataatca acaccatcag
    39601 ggatttccca caacgcatga cgatgaattt tgagaatttc accaggaacc agacaatatt
    39661 tggaaaaacc accgccccag gtattattat ccagaccaag gtttactttt tctgtacaac
    39721 acagaaaatc accttgttca caggccggac aaacgccgca aacgtgaccg ctgttatccg
    39781 atacgacgcg ttgccccact ttccagtctt tgactttttc accaacctgc gcaatacaac
    39841 ctgcgaactc atggccgcgg atagagttaa actcatcaga accgctatcg acattgtagt
    39901 gcttcatgtc tgcgccgcag attgccgcgg ctttaatttc aatcactaca tcttccgggc
    39961 cacacatggg ttgtgggaca tcaatcatct tgtagccgcc aaaggctttg ccaaaccgag
    40021 ccagtgcttt cattgcgtca tcctctgtgt tttgttttgc ttagatggtc atcatactta
    40081 cccacatgat ttttgaggtc aacattaaat ctgtaaaaca cagataatga tctgcgtttt
    40141 acaactcaga tcacaattac gcagaaaatg gctggttctg ttacaggttg atggaaggct
    40201 gaacgcaaaa ggagcaaatt tgagagggat taccggccaa ataggtctga gacttaagtt
    40261 caggagaagt cattcaggcg tgaacgcgac ggcaagagac cgggctgggg ctttgcctgg
    40321 tcgtgacgag ataattgaga tgagtcgtgg ggttattgtt ggctatcccc tttataaccg
    40381 cggcaaaaag gggacaaacc acttacttaa acatggcaat aggctttgct gagataccaa
    40441 aatcttcttt taactgttgt ttgctcttca tgaccatctg accatttgtg tcaattgtca
    40501 tatgctgtgc ttcggtatta ttgcgggctt gccacagcat caccagttgc aggcagttct
    40561 ctttttgttc ctcggtcaac gcaacgccat caggccattt ccccagttca acggcggtcg
    40621 acaaacgctg gtatacctca ggcgtcatgc tgttgataat gtcatcaaga ttcataattt
    40681 ttccgctcct gtggaataag ttgctgaatc gttttttcaa ccgttgattt cttcgccgtt
    40741 ttcgccatcg gtaaagcgta aagaagcaga gttaacacaa taacgttcgc ccgttggctg
    40801 cggcccgtca gggaagacat gccccagatg ggcatcacag ttaccgcaac gaatttctat
    40861 gcgctgcatt ccatgtgaca agtctttgat ataacgaatg gattcttcac tcaccggttc
    40921 gtagaaactg ggccagccac agccggaatc atacttggtt tgggaatgaa acagcggggc
    40981 atcgcagatc aaacagtgat atacgccgtc acgcttgtta tgcagtaaac gacccgtaaa
    41041 tggcggctct gtcccatgat tctgcgtcac gtaaaactgc atctcggaca aatttttttt
    41101 cagttcttct gccgaaggtt tattagccat ttgctcacat ctcactttaa tcgtgctcac
    41161 attacgtgac tgattctaac aaaacattaa caccaactgg caaaattttg tcctaaactt
    41221 gatctcgacg aaatggctgc acctaaatcg tgatgaaaat cacattttta tcgtaattgc
    41281 cctttaaaat tcggggcgcc gaccccatgt ggtctcaagc ccaaaggaag agtgaggcga
    41341 gtcagtcacg taatgcttag gcacaggatt gatttgtcgc aatgattgac acgattccgc
    41401 ttgacgctgc gtaaggtttt tgtaatttta caggcaacct tttattcact aacaaatagc
    41461 tggtggaata tatgactatc aaagtaggta tcaacggttt tggccgtatc ggtcgcattg
    41521 ttttccgtgc tgctcagaaa cgttctgaca tcgagatcgt tgcaatcaac gacctgttag
    41581 acgctgatta catggcatac atgctgaaat atgactccac tcacggccgt ttcgacggta
    41641 ccgttgaagt gaaagacggt catctgatcg ttaacggtaa aaaaatccgt gttaccgctg
    41701 aacgtgatcc ggctaacctg aaatgggacg aagttggtgt tgacgttgtc gctgaagcaa
    41761 ctggtctgtt cctgactgac gaaactgctc gtaaacacat caccgctggt gcgaagaaag
    41821 tggttatgac tggtccgtct aaagacaaca ctccgatgtt cgttaaaggc gctaacttcg
    41881 acaaatatgc tggccaggac atcgtttcca acgcttcctg caccaccaac tgcctggctc
    41941 cgctggctaa agttatcaac gataacttcg gcatcatcga aggtctgatg accaccgttc
    42001 acgctactac cgctactcag aaaaccgttg atggcccgtc tcacaaagac tggcgcggcg
    42061 gccgcggcgc ttcccagaac atcatcccgt cctctaccgg tgctgctaaa gctgtaggta
    42121 aagtactgcc agaactgaat ggcaaactga ctggtatggc gttccgcgtt ccgaccccga
    42181 acgtatccgt ggttgacctg accgttcgtc tggaaaaagc tgcaacttac gagcagatca
    42241 aagctgccgt taaagctgct gctgaaggcg aaatgaaagg cgttctgggc tacaccgaag
    42301 atgacgtagt atctaccgat ttcaacggcg aagtttgcac ttccgtgttc gatgctaaag
    42361 ctggtatcgc tctgaacgac aacttcgtga aactggtatc ctggtacgac aacgaaaccg
    42421 gttactccaa caaagttctg gacctgatcg ctcacatctc taaataagtt gagatgacac
    42481 tgtgatctaa aaagagcgac ttcggtcgct ctttttttta cctgataaaa tgaagttaaa
    42541 ggactgcgtc atgattaaga aaatttttgc ccttccggtc atcgaacaaa tctcccctgt
    42601 cctctcccgt cgtaaactgg atgaactgga cctcattgtg gtcgatcatc cccaggtaaa
    42661 agcctctttt gcattacagg gcgcacacct tctctcgtgg aaacctgcgg gtgaagaaga
    42721 agttctgtgg ttgagcaaca acacaccgtt caaaaatggc gtcgctattc gcggtggcgt
    42781 accggtttgc tggccgtggt ttggtccggc tgcacaacaa ggtctgcctg cgcacggttt
    42841 tgcccgcaac ctgccgtgga cgctgaaatc acatcatgaa gatgctgatg gcgtagcgct
    42901 gacttttgaa ttgacacaaa gcgaagagac gaaaaaattc tggccgcacg actttacgct
    42961 gttagcgcat ttccgcgtgg gtaaaacctg tgaaatcgat cttgaagcac atggcgaatt
    43021 tgaaaccacc tctgccctgc atacctactt taacgtgggt gatatcgcta aggtaagcgt
    43081 cagtgggctg ggcgatcgct tcattgataa agtaaatgac gcgaaagaag atgtactgac
    43141 cgacggtatt cagaccttcc ctgaccgtac cgatcgcgtg tatctgaatc cgcaagattg
    43201 cagcgtgatt aatgatgaag cgctgaatcg tattatcgcc gtaggccacc agcatcatct
    43261 gaacgttgtc ggctggaacc cgggaccggc gctttcagtt agcatgggcg acatgccgga
    43321 tgatggctac aaaacatttg tttgtgtaga aacggcttac gcttcagaaa cgcaaaaagt
    43381 gaccaaagag aaacctgcac atctggcgca atccattcgc gttgcgaaac gttaatttac
    43441 gttaatgttg tgtgccgggt gcgatgcatc cggcacgtat caacatcaca ccatatccag
    43501 cgcagttttt ccttttggtg ccggatatgc cttatccagc atagctaatt ccgctgaaga
    43561 aagttcgacc tcaagcacag ccgcattttg ttggacatgg gcaatcgtgg ccgcttttgg
    43621 aatcgccatc acaccctgat gactgatcac ccacgccaac aatacttgtg ccgcgctgat
    43681 attgtgagca tgtgcaattt cgttgactac cgcgtttttt aacagtccat tgcgcaaccg
    43741 cccggcctgg gctaatggac tgtatgccat caccggcatc tgctgttgct ggcaccaggg
    43801 gagtagatcg tactcaatcc ctcgtgaacc gagatggtaa agcacctgat tagtggcaca
    43861 ctgatttccc cccggcagct gccagagttc ctgcatatca gcataatcaa ggttcgaaac
    43921 gccccagcgg cggatttttc cctgggcgat caaattttcc atcgctgcaa cagtctcttc
    43981 aaaagcgaaa ctgccagacc agtgtaataa gtagagatcg agataatcag tattgagacg
    44041 gcgtaaactg gcttcgcatg catttatcgc tttttgcccg ccagcattcc acggatagac
    44101 tttagagacg agaaagacgt tatcgcgcag accggttaat gcttccccaa ccaccttttc
    44161 ggcaccgcca tcggcataca tttcggcggt atcaatgagg gttaaaccga gctcaatgcc
    44221 cgcgcgtagt gcagcaactt ctgttttgcg ctgactggca tcttcgccca tataccatgt
    44281 tccctgccct atggctggca gtgagacatc gccactaaat tgaatcattt tttgttgcat
    44341 tgtttcctcc caggtattgc accaccgtaa tgcaaaacag ggcgtgacgc cctgttttta
    44401 tgcacaaaat gccctggaaa gatgcattat cagaatttgt aggtgatccc ggtagaaatc
    44461 aggccagtcc aggatttatc caccatcggg ctgtcagtaa cttcatcaga cagacgggtg
    44521 tagcgcgcgg taccgtaaac actccagtcg ccgaggaagt tgtagctggc gctcagctcc
    44581 aggtaagggc tccagctgtc gttcgggtta tagccacgca gaccgctgcg agcggactct
    44641 ttgcgcgata cgccataata gtattcgttc tggttttcgc tgttccactg cacaccaata
    44701 cccggagtca cggtcaggcc accgttggtg taacgataca accaggccat atcccagacg
    44761 atgccgttgc tgttatccag ggtatcgcca gccagggtgg tacgcaggta accgtactgg
    44821 gtaaagtgag cataagacag accagccatc atggtgctct tacggtcatc caggtgacgc
    44881 atttggtgat cgccactgtc tttagctttg aagtaaagcg gcgaccagta agcggtaatt
    44941 gaaagtttat ccgttgcgtc attccacagg tagtaaccac cacctaagcc acggaaccag
    45001 aagttatcgc cttcatagtt gattaccggt actgggtaaa catcggtatc gtaatcttta
    45061 tatgggtgtt caacgacacc tacgcctgcg cccagggaaa atttaccttc agcgtgcgct
    45121 acgcctgcag acgttgcgat aagcactcca agtgccagaa gtttgagttt ggtcacaatt
    45181 aatcattcct taaacaaatg tttagcgggc gacaaagttt acccgtcaat acatcgaaac
    45241 ccaacctttt tacgttttca ttttttaaag taactgttta attttcctga cgcggatgac
    45301 accgcgctta cagccaaatg aatttagcgt tactggcgag cctggtcttt acattaatta
    45361 tgcaaaattt atggatgagt tgttgatatg ccattgaaat taagaaagcc gtgcaggcaa
    45421 gttttccatt tgccatctac gcttaatttt gaaggtgtat caccgggcac gttgttctca
    45481 tcgtcgataa aatggcatga gagttgctgt gttttagcaa gagacgtcgt tcagtttacc
    45541 tcttccggga gcctctacta ttcatatgaa cggctcttaa cctgtgctaa aaaacgaaag
    45601 gacggcatac catgaatata ttcgatcact atcgccagcg atatgaagct gccaaggacg
    45661 aagagttcac actgcaggag tttcttacca cttgtcggca agatcgcagt gcttatgcca
    45721 acgcggctga gcggctattg atggctatcg gtgagcctgt catggtcgat acagcccagg
    45781 aacccagact ttctcgactc ttttctaacc gggtcattgc acgttatccg gcgtttgaag
    45841 agttttacgg catggaagac gcgattgaac agattgtctc ttatctgaaa cacgcggctc
    45901 aggggctgga agagaagaaa caaatcctgt atctgctggg gcctgtgggt gggggtaaat
    45961 catcgcttgc tgagcgactg aaatcattaa tgcagctcgt accgatttat gtattgagcg
    46021 ccaacggtga gcgtagcccg gtcaacgatc atccgttctg tcttttcaat ccgcaggaag
    46081 atgcgcagat tctggaaaaa gagtatggca ttcctcgccg ttatctcggc accatcatgt
    46141 cgccgtgggc ggcaaaacgt ctgcatgaat ttggtggcga tattactaag ttccgggtag
    46201 tgaaggtctg gccgtccatt ctgcaacaaa ttgctatcgc caaaacagag cccggtgatg
    46261 agaacaacca ggacatctcc gcgctggtag ggaaagtcga tattcgtaaa ctcgaacact
    46321 acgcgcagaa tgacccagac gcctatggct attccggcgc actgtgccgc gccaaccagg
    46381 ggatcatgga attcgttgag atgtttaaag caccgattaa agtgctgcat cccttgttaa
    46441 ccgccactca ggaaggtaac tacaacggga cggaaggtat ctccgccctg ccgttcaacg
    46501 ggattattct tgcccactcg aacgagtccg aatgggtcac tttccgtaat aacaaaaaca
    46561 acgaagcctt cctcgatcgt gtttacatcg tgaaggtgcc gtattgcttg cgcatttccg
    46621 aagagatcaa aatctacgag aaattgctta atcacagtga attgactcac gccccatgcg
    46681 cccctggcac tctcgaaaca ctgtcacgtt tttccattct ttcgcgcctg aaagagccag
    46741 aaaactccag catttattca aagatgcggg tttatgatgg cgaaagtctg aaagacaccg
    46801 atcccaaagc caagtcgtat caggaatatc gtgactacgc cggtgtcgat gaagggatga
    46861 acggtctgtc gacgcgtttt gcgtttaaga tcctctcccg cgtgttcaac ttcgatcatg
    46921 tagaagtggc ggcaaacccg gtccatctgt tctacgtcct ggaacagcag atcgagcgcg
    46981 agcagttccc acaagagcag gcagaacgct atctggagtt cctgaaaggt tatctgatcc
    47041 cgaaatatgc cgagtttatc ggcaaagaga tccagacggc ctaccttgaa tcctattccg
    47101 aatatgggca aaacattttc gaccgttatg ttacctacgc ggatttctgg attcaggatc
    47161 aggagtatcg cgatccggat accgggcagc tgtttgaccg cgagtctctt aacgccgaac
    47221 tggagaaaat cgagaaaccg gcggggatca gtaatccaaa agatttccgc aacgagattg
    47281 ttaacttcgt actgcgcgcc agagccaata acagcggacg caatccgaac tggaccagct
    47341 atgaaaaact gcgcacggtt atcgagaaga aaatgttctc caataccgag gagctgttgc
    47401 cggttatctc gtttaacgcc aaaacgtcaa ccgacgagca gaagaaacac gacgactttg
    47461 tcgaccgtat gatggaaaaa ggctacaccc gtaaacaggt gcgtttactg tgcgaatggt
    47521 atttgcgcgt acgtaaatcg tcttaacaac cctggcccgg tacacatgct accgggccta
    47581 caacgacagc gaaccgtggg ccagagaaat ggcaacacag gcgcgtagca tacagttggc
    47641 aaatgtagta cggggggcat atgacctggt ttattgaccg gcgtctgaac ggcaaaaaca
    47701 aaagcatggt gaatcgccag cgttttttac gccgttataa agcgcaaatt aaacagtcga
    47761 tctccgaggc cattaataag cgttcggtga ctgacgtcga cagcggcgaa tccgtatcca
    47821 ttcccacgga agatattagc gaaccgatgt ttcatcaggg gcgtggcggc ctgcgccacc
    47881 gcgtacatcc gggcaatgac catttcgtcc agaacgaccg aattgaacgt ccccagggtg
    47941 gcggcggagg ttccggcagt ggtcagggcc aggccagcca ggatggtgaa ggtcaggatg
    48001 aattcgtctt tcagatttcg aaagatgagt atcttgatct gctctttgaa gatttggcct
    48061 taccgaatct gaaacaaaac caacaacgcc agctgaccga atataaaacg catcgggcgg
    48121 gttataccgc taacggcgtt ccggccaata tcagcgttgt gcgttcattg cagaactcac
    48181 tggcgcgacg cacagccatg acggcaggca agcggcggga acttcatgca ctggaagaga
    48241 atttggccat catcagcaac agtgaacctg cgcagctgct ggaagaggaa cgtctgcgca
    48301 aagaaattgc agaattacgt gccaaaattg aacgcgtccc ttttattgac accttcgatt
    48361 tacgttacaa gaactacgag aagcggcccg atccctccag ccaggcagtg atgttttgcc
    48421 tgatggacgt ttccggttca atggatcaat ccactaaaga tatggctaag cgtttttata
    48481 ttctactgta tctgttcctc agcagaacgt ataagaacgt ggaagtcgta tacatccgcc
    48541 atcataccca ggcgaaagaa gtcgatgaac atgagttttt ctactcgcag gaaactggcg
    48601 gcaccattgt ttccagcgcc ctgaaactga tggatgaggt agtgaaagag cgttataacc
    48661 cggcacagtg gaatatttac gctgcacaag catcggacgg cgataactgg gccgatgact
    48721 ctccgctttg ccatgaaatt ctggcgaaaa aattattacc cgtggttcgt tattacagct
    48781 atatcgaaat tacccgtcgc gcgcatcaga cactgtggcg agaatatgag catctgcaat
    48841 ctactttcga caactttgcg atgcagcata tccgcgacca ggatgatatt tatccggtgt
    48901 tccgtgaact gtttcataag caaaatgcaa cagctaaaga ctaaaactat cagccgggtc
    48961 attatcgcct ggctgatttt tagctcactg taaattatct catttattac atacattagc
    49021 ttacaa
//
LOCUS       DAZFFG010000021        46771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFFG010000021 DAZFFG010000000
VERSION     DAZFFG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.021"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(263..805)
                     /gene="ycfP"
                     /locus_tag="ACHJ7J_001567"
     CDS             complement(263..805)
                     /gene="ycfP"
                     /locus_tag="ACHJ7J_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO4999819.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(828..1853)
                     /gene="nagZ"
                     /locus_tag="ACHJ7J_001568"
     CDS             complement(828..1853)
                     /gene="nagZ"
                     /locus_tag="ACHJ7J_001568"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO4999820.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1864..2688)
                     /gene="thiK"
                     /locus_tag="ACHJ7J_001569"
     CDS             complement(1864..2688)
                     /gene="thiK"
                     /locus_tag="ACHJ7J_001569"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO4999821.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2669..3310)
                     /gene="lpoB"
                     /locus_tag="ACHJ7J_001570"
     CDS             complement(2669..3310)
                     /gene="lpoB"
                     /locus_tag="ACHJ7J_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO4999822.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3324..3701)
                     /gene="ycfL"
                     /locus_tag="ACHJ7J_001571"
     CDS             complement(3324..3701)
                     /gene="ycfL"
                     /locus_tag="ACHJ7J_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO4999823.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3704..4063)
                     /gene="hinT"
                     /locus_tag="ACHJ7J_001572"
     CDS             complement(3704..4063)
                     /gene="hinT"
                     /locus_tag="ACHJ7J_001572"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO4999824.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4397..6586
                     /gene="fhuE"
                     /locus_tag="ACHJ7J_001573"
     CDS             4397..6586
                     /gene="fhuE"
                     /locus_tag="ACHJ7J_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO4999825.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6646..8079)
                     /gene="ptsG"
                     /locus_tag="ACHJ7J_001574"
     CDS             complement(6646..8079)
                     /gene="ptsG"
                     /locus_tag="ACHJ7J_001574"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO4999826.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8374..9171)
                     /gene="ycfH"
                     /locus_tag="ACHJ7J_001575"
     CDS             complement(8374..9171)
                     /gene="ycfH"
                     /locus_tag="ACHJ7J_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4999827.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9182..10186)
                     /gene="holB"
                     /locus_tag="ACHJ7J_001576"
     CDS             complement(9182..10186)
                     /gene="holB"
                     /locus_tag="ACHJ7J_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO4999828.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10183..10824)
                     /gene="tmk"
                     /locus_tag="ACHJ7J_001577"
     CDS             complement(10183..10824)
                     /gene="tmk"
                     /locus_tag="ACHJ7J_001577"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4999829.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10814..11836)
                     /gene="yceG"
                     /locus_tag="ACHJ7J_001578"
     CDS             complement(10814..11836)
                     /gene="yceG"
                     /locus_tag="ACHJ7J_001578"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO4999830.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11839..12648)
                     /gene="pabC"
                     /locus_tag="ACHJ7J_001579"
     CDS             complement(11839..12648)
                     /gene="pabC"
                     /locus_tag="ACHJ7J_001579"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO4999831.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12768..14009)
                     /gene="fabF"
                     /locus_tag="ACHJ7J_001580"
     CDS             complement(12768..14009)
                     /gene="fabF"
                     /locus_tag="ACHJ7J_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO4999832.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14097..14333)
                     /gene="acpP"
                     /locus_tag="ACHJ7J_001581"
     CDS             complement(14097..14333)
                     /gene="acpP"
                     /locus_tag="ACHJ7J_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4999833.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14544..15278)
                     /gene="fabG"
                     /locus_tag="ACHJ7J_001582"
     CDS             complement(14544..15278)
                     /gene="fabG"
                     /locus_tag="ACHJ7J_001582"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO4999834.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15291..16220)
                     /gene="fabD"
                     /locus_tag="ACHJ7J_001583"
     CDS             complement(15291..16220)
                     /gene="fabD"
                     /locus_tag="ACHJ7J_001583"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO4999835.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKTPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16236..17189)
                     /gene="fabH"
                     /locus_tag="ACHJ7J_001584"
     CDS             complement(16236..17189)
                     /gene="fabH"
                     /locus_tag="ACHJ7J_001584"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO4999836.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17257..18327)
                     /gene="plsX"
                     /locus_tag="ACHJ7J_001585"
     CDS             complement(17257..18327)
                     /gene="plsX"
                     /locus_tag="ACHJ7J_001585"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO4999837.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRSSRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18408..18581)
                     /gene="rpmF"
                     /locus_tag="ACHJ7J_001586"
     CDS             complement(18408..18581)
                     /gene="rpmF"
                     /locus_tag="ACHJ7J_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO4999838.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18633..19154)
                     /gene="yceD"
                     /locus_tag="ACHJ7J_001587"
     CDS             complement(18633..19154)
                     /gene="yceD"
                     /locus_tag="ACHJ7J_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO4999839.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19353..19937
                     /gene="yceF"
                     /locus_tag="ACHJ7J_001588"
     CDS             19353..19937
                     /gene="yceF"
                     /locus_tag="ACHJ7J_001588"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO4999840.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20049..21008)
                     /gene="rluC"
                     /locus_tag="ACHJ7J_001589"
     CDS             complement(20049..21008)
                     /gene="rluC"
                     /locus_tag="ACHJ7J_001589"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO4999841.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYVFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21581..24766
                     /gene="rne"
                     /locus_tag="ACHJ7J_001590"
     CDS             21581..24766
                     /gene="rne"
                     /locus_tag="ACHJ7J_001590"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO4999842.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMQDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEDTVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24961..25914)
                     /gene="flgL"
                     /locus_tag="ACHJ7J_001591"
     CDS             complement(24961..25914)
                     /gene="flgL"
                     /locus_tag="ACHJ7J_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO4999843.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25926..27569)
                     /gene="flgK"
                     /locus_tag="ACHJ7J_001592"
     CDS             complement(25926..27569)
                     /gene="flgK"
                     /locus_tag="ACHJ7J_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO4999844.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGVVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDKTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27635..28576)
                     /gene="flgJ"
                     /locus_tag="ACHJ7J_001593"
     CDS             complement(27635..28576)
                     /gene="flgJ"
                     /locus_tag="ACHJ7J_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO4999845.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTSAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKIVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28576..29673)
                     /gene="flgI"
                     /locus_tag="ACHJ7J_001594"
     CDS             complement(28576..29673)
                     /gene="flgI"
                     /locus_tag="ACHJ7J_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO4999846.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29685..30383)
                     /gene="flgH"
                     /locus_tag="ACHJ7J_001595"
     CDS             complement(29685..30383)
                     /gene="flgH"
                     /locus_tag="ACHJ7J_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO4999847.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGAVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30436..31218)
                     /gene="flgG"
                     /locus_tag="ACHJ7J_001596"
     CDS             complement(30436..31218)
                     /gene="flgG"
                     /locus_tag="ACHJ7J_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO4999848.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31390..32145)
                     /gene="flgF"
                     /locus_tag="ACHJ7J_001597"
     CDS             complement(31390..32145)
                     /gene="flgF"
                     /locus_tag="ACHJ7J_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO4999849.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAERQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32165..33370)
                     /gene="flgE"
                     /locus_tag="ACHJ7J_001598"
     CDS             complement(32165..33370)
                     /gene="flgE"
                     /locus_tag="ACHJ7J_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO4999850.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWEVYTQDSSDP
                     TGTADHAMTLVFNSNGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33395..34090)
                     /gene="flgD"
                     /locus_tag="ACHJ7J_001599"
     CDS             complement(33395..34090)
                     /gene="flgD"
                     /locus_tag="ACHJ7J_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO4999851.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34102..34506)
                     /gene="flgC"
                     /locus_tag="ACHJ7J_001600"
     CDS             complement(34102..34506)
                     /gene="flgC"
                     /locus_tag="ACHJ7J_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO4999852.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34510..34926)
                     /gene="flgB"
                     /locus_tag="ACHJ7J_001601"
     CDS             complement(34510..34926)
                     /gene="flgB"
                     /locus_tag="ACHJ7J_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO4999853.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35081..35740
                     /gene="flgA"
                     /locus_tag="ACHJ7J_001602"
     CDS             35081..35740
                     /gene="flgA"
                     /locus_tag="ACHJ7J_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO4999854.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVTGQVVSGVVDADGNILIN
                     L"
     gene            35816..36109
                     /gene="flgM"
                     /locus_tag="ACHJ7J_001603"
     CDS             35816..36109
                     /gene="flgM"
                     /locus_tag="ACHJ7J_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO4999855.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36114..36530
                     /gene="flgN"
                     /locus_tag="ACHJ7J_001604"
     CDS             36114..36530
                     /gene="flgN"
                     /locus_tag="ACHJ7J_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO4999856.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(36570..38105)
                     /gene="murJ"
                     /locus_tag="ACHJ7J_001605"
     CDS             complement(36570..38105)
                     /gene="murJ"
                     /locus_tag="ACHJ7J_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO4999857.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38215..39138)
                     /gene="yceM"
                     /locus_tag="ACHJ7J_001606"
     CDS             complement(38215..39138)
                     /gene="yceM"
                     /locus_tag="ACHJ7J_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4999858.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSFSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39140..39787)
                     /gene="yceH"
                     /locus_tag="ACHJ7J_001607"
     CDS             complement(39140..39787)
                     /gene="yceH"
                     /locus_tag="ACHJ7J_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO4999859.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDADLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39798..40382)
                     /gene="rimJ"
                     /locus_tag="ACHJ7J_001608"
     CDS             complement(39798..40382)
                     /gene="rimJ"
                     /locus_tag="ACHJ7J_001608"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4999860.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40618..41826
                     /gene="mdtH"
                     /locus_tag="ACHJ7J_001609"
     CDS             40618..41826
                     /gene="mdtH"
                     /locus_tag="ACHJ7J_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO4999861.1"
                     /translation="MSCVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41890..42537
                     /gene="grxB"
                     /locus_tag="ACHJ7J_001610"
     CDS             41890..42537
                     /gene="grxB"
                     /locus_tag="ACHJ7J_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO4999862.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNVSDDLRALDKLIVK
                     PNAVNGQLSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42671..43231
                     /gene="yceB"
                     /locus_tag="ACHJ7J_001611"
     CDS             42671..43231
                     /gene="yceB"
                     /locus_tag="ACHJ7J_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4999863.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43337..44383
                     /gene="pyrC"
                     /locus_tag="ACHJ7J_001612"
     CDS             43337..44383
                     /gene="pyrC"
                     /locus_tag="ACHJ7J_001612"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO4999864.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESTCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVCEEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44457..44702
                     /gene="dinI"
                     /locus_tag="ACHJ7J_001613"
     CDS             44457..44702
                     /gene="dinI"
                     /locus_tag="ACHJ7J_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO4999865.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            44992..45246
                     /gene="bssS"
                     /locus_tag="ACHJ7J_001614"
     CDS             44992..45246
                     /gene="bssS"
                     /locus_tag="ACHJ7J_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO4999866.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45361..46479
                     /gene="solA"
                     /locus_tag="ACHJ7J_001615"
     CDS             45361..46479
                     /gene="solA"
                     /locus_tag="ACHJ7J_001615"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO4999867.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGVKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPDHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQNKKSDFDLTPFRLSRFQ"
     gene            46527..46640
                     /locus_tag="ACHJ7J_001616"
     CDS             46527..46640
                     /locus_tag="ACHJ7J_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO4999868.1"
                     /translation="MRRLLHYLIKNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        11355 a        12585 c        11598 g        11233 t
ORIGIN      
        1 aggttacagc aatgaaaatg ttcttcgcta ttgagaataa catgaatggt gcattggtca
       61 taccaaaatt gatgttaatc aagttttgtt gctgaaaaga taggcgactt taccgggtga
      121 gatcaaaaaa cagagtgtat tgtttattat ttgagcgaat ttgtgagagt gatatagagt
      181 gaggggtgta tgcagggcgt tcggtaggcc tgataagagg cgcatcaggt accggcaagg
      241 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat
      301 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
      361 gatgcagctc ttcagatgtc cgctggctgt taagcgcttc atcattacgc gacaaaatca
      421 ccaggcaacg atcgcgattc ttctcacgga agttggtcac acacttagtg gcaatatcgg
      481 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
      541 tgaagattac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
      601 gaccaacgcc gcaaatcagc ggacgctcgt cgacgttcag ttgcaacatt ttgtccactt
      661 ctttgagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
      721 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
      781 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctgct
      841 ttctcttcct gccagcgttc atgtaactga ttcagacggg cgctgatcgc tttccagcga
      901 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
      961 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
     1021 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
     1081 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt
     1141 tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca
     1201 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
     1261 ctgaagaccg acatatcttt ggcacgaatt tccgcttgcg ggcgtgggtc gcacggcgtc
     1321 tctttgtgtg aatctgcagt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
     1381 attccggctt catgcatccc atcaataaac cgactggcga ttgccagggc tttttctggg
     1441 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gtcctacatc cagcactggc
     1501 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
     1561 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
     1621 aagcgggtaa aaccttcgcg aaaacgctgg acgcgtccac cttcctgatc taccgccacc
     1681 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgcaa ctgggcagga
     1741 tcatgatagt ttcgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
     1801 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
     1861 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
     1921 atcgccggtt tgtcgccagc ggtactcaaa ccaccctgct ttgagcatca gcagccaggg
     1981 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
     2041 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
     2101 cgcgatatca ccatctcctg catactccca gtcgatgagt tttaaccctg acgcgctatg
     2161 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gccgtggttc
     2221 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc actgtccgcc gcgccggatc
     2281 gctttgctgc cagtacagtt ccagtaacgg caacagcgtt attcgccagc caaaacgcgg
     2341 ttgttgatgt agataataca gcaagcctgc cagttcgtta gtatccggca aatacgtttt
     2401 tacctcgccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
     2461 tgcaatgcat gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
     2521 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
     2581 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
     2641 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
     2701 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
     2761 tgccagaggc gctggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
     2821 ctttactacg ggtgcctaaa ctatcctgcg gcgacaaacc taactgttgc ttagccatcg
     2881 acagctgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
     2941 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
     3001 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
     3061 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgcagtt tgatcttcgt
     3121 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
     3181 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
     3241 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
     3301 tttttgtcat caagattcac cccttacaaa tatagataaa gtctgacttt gtgcgccccc
     3361 agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt aacgctgcgt
     3421 ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa acgataatga
     3481 acggtaacgg gttcttgcct ttcgttataa agcgttgagg atgctgaagg ttgaatatca
     3541 gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga tgactccatc
     3601 accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc caccagcagt
     3661 aacaacgcca gagacaccag cccaaagcat ccttttctca tcgttacaga cctttatgcg
     3721 ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg taaacctctt
     3781 gtccgccatg gcggttggta ttcatgatca gacgataacc atcttcggca ataccttctt
     3841 gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca tgctcagctg
     3901 acacgtcgtt cacagtcgga atgaggatat tcggaatgat cagaatatgc gttggcgctt
     3961 gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata tcggagggga
     4021 tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt tcctttttca
     4081 aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga gtatggtcga
     4141 gttaaacctg ctctttcaac cttaactcac gatttcttaa gcaaaaaatc gttttgctgc
     4201 cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt
     4261 ttggtttaca cgacaaatat gaatgcgtat atttctcatt tgcatttaca aacaaaatta
     4321 ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga cgacaacata
     4381 aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat atcaagccat
     4441 caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt ctgccgcttt
     4501 tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag ctccagatga
     4561 tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc agatgactca
     4621 acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag atcagcagtt
     4681 acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc aggcggattc
     4741 cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg ttgatggtat
     4801 ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata tggcactatt
     4861 tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg gtaatccatc
     4921 tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt gaatttaaag gcgatgtctc
     4981 ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa gcccactcac
     5041 cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg actcatggct
     5101 ggaccgctac aatagtgaaa agaccttctt ctccggcatt gtcgatgctg atttaggcga
     5161 tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata gcccaacctg
     5221 gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc gcgcacgcag
     5281 taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta tgaccctgaa
     5341 gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg aagtcgaatt
     5401 tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta tgctggttgg
     5461 gccatacagt aattatggac ctggctttga ttatgtcggc ggcaccggtt ggaacagtgg
     5521 caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat tgtttggtcg
     5581 tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt acttcagttc
     5641 atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg gcaatttccc
     5701 acaaaccgac tggtcaccac agagcctggc gcaggacgat accacacata tgaaatcgtt
     5761 atatgctgcc actcgtgtca cccttgccga tccgctgcat ctgatcctcg gcgcacgtta
     5821 taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca ccacgcctta
     5881 cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct atacctctat
     5941 tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa tcaccggtaa
     6001 caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca ccacgttagc
     6061 catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta tccccggcag
     6121 caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaaggtg tggaatttga
     6181 actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc gctatattgc
     6241 agaggataac gaaggaaacg ccgttaatcc taatctgcct cgcaccacgg ttaaaatgtt
     6301 caccagctac cggttgcctg tcatgccaga gttgacggtc ggcggtggtg ttaactggca
     6361 aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag
     6421 ctacgcgctg gtggatctct tcacccgcta ccaggtgacg aaaaacttct cgttacaggg
     6481 gaacgtcaat aacctgttcg acaaaaccta cgataccaac gtggaaggtt ctatcgtcta
     6541 cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa
     6601 aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acggattagt ggttacggat
     6661 gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatcg cctgaacacc
     6721 agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccggcct gatccacttt
     6781 agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt
     6841 ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac ctgtcgcttt
     6901 tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt taatcagcac
     6961 gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggga acagccacag
     7021 tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaga acgacgtacc
     7081 gtcacgcatc cccagaagaa tacagattgg gaatgccaag cctgccagaa tcgcgtggat
     7141 gatgtacagg atcggcgcaa cgaacatgaa ggagaactcg atcggctcgg tgataccggt
     7201 caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcgc ggttttctgg
     7261 tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt tgaacaagaa
     7321 gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa tgtcgccgtg
     7381 gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag gtacgttcca
     7441 gatgtggtgc agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc
     7501 aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag aaccaatcgg
     7561 cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag aaatgatcgg
     7621 cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac ggtagaaacg
     7681 gttaaacatg tacgctgcga tcgcaccgga gataatccct ccgagtacgc cagtatccgc
     7741 caggtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg caaccacggc
     7801 catggtttta accatgatgc cataggcaac aactgcggcc agcgcggata cgccatcgtt
     7861 attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg caaagacgga
     7921 accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga aattcgcgga
     7981 accgacgccc agcagaatac ctgcgatagg cagtacggat accggcagca tcagcgattt
     8041 accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg ctcctgagta
     8101 tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg
     8161 catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa tttgtttgac
     8221 ggctatcacg tttcaacttc agatcattgg agaagttgta cattttgaca aaacatatgt
     8281 cacaatattt caaacgtggt tattcaccgc ccaattttcg gcggtgaact ttactcgttt
     8341 tagccattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa ggcgggaagc
     8401 gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt cttcaacggc
     8461 aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag gttgattctc
     8521 ttttcctcga tgcggcaccg gcgcaaggta aggtgagtca gtttccacca gtaaccgatc
     8581 caaggggaca taacgcgcag catcgcgcag ttgctccgca tttcggaagg tcacaatgcc
     8641 ggaaaaggag atgtaaaatc cgagatccag taatttgccc gccgtttctc tgtcctctgt
     8701 aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt
     8761 atcggcgcgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac gaccaatctg
     8821 gatatgatgg atgaaggact cttgctgacg tactttagtt tccggtgtgt aataataatc
     8881 cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc gtaaatcttc
     8941 tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata cgacgttgtc
     9001 acgttcgcct accagatccc gcatgtgtaa ataacccggt aatgttgtgg cgactgccag
     9061 acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt ccttatgcaa
     9121 agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca tgatgtctct
     9181 cttaaagatg aggaaccggt agcacaacgc ccggttgcag gtaatgctca atacgcagta
     9241 aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac tgttcacgaa
     9301 tgtggcaaac atcccccagt atagcctgca ggcgcgaggg agaaagatgg tttgccagtt
     9361 cgacgaccag gcccggcaca tcaacattgg tcacctgcgc agcgccatga tggcgtttta
     9421 gcgcatccat cagcaacgtt gccagccagt gcaaacgcgc cggagcttgt tcatgattaa
     9481 gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaac gcctgacaca
     9541 atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac
     9601 cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact tcgcgtgaaa
     9661 gccaggtcac ggcgtactgt tccggcggcg gcgcaaggta atgtaaccga caacgactac
     9721 gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag gtttctgctg
     9781 gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat
     9841 cggttaccca gacaactttc gcaccaccta ggcgtgcgtg ctcattcagc ttttcggtga
     9901 cctcacgtac cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc agggtgtagt
     9961 aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg caacttttgt
    10021 ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa gcatcatcgc
    10081 ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc tgatagctgg
    10141 ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt ccaactcctt
    10201 cacccagtgg gtcacggtag tgcggattgc atccatcacg gcctccagcg gctgggtggc
    10261 atcaatggta tgaatacttt tatcttgtgc agccagttcc agatagcggg cgcgggtgcg
    10321 attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg
    10381 ttttaagcca acttccgggg taacatcgag atagagcgtt aagtcggggc gaaaatcccc
    10441 gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc ctccgccctg
    10501 atacgcctga gtggagagat cgtggcgatc gccaatcacc caggtgccgt tagccagcgc
    10561 aggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca gaacttcggc
    10621 tttatcggta atgacttcat cgcctaccga tttgatatcc agcaccaggc ttcttaactt
    10681 ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga tacccagttg
    10741 ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt ccagcccctc
    10801 aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa tcctgcacag
    10861 acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca tcggccacaa
    10921 aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc gcccccggcg
    10981 tagctattgc acctggcggc aaaccggtaa tggtataggt gttatacgct gtcggcgttt
    11041 ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa atcacggtcg
    11101 ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag gcaacctgat
    11161 cgcgttcact ggcaacggcg gtttcttttt cgataattga tgccatcgtc accagctgat
    11221 ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg accgctttca
    11281 ccattttctt gtgcgctcgc ttgagtaacg cgacatcggt ggtattggcg gtatacatcc
    11341 aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt ttccagttca agtgcctgcg
    11401 ctacggtggc gtacttatcg tcgctcagcg tgtgcttgat atacggagcc tcacgcaatt
    11461 gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt
    11521 taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta aagcggtaag
    11581 tcccggcttt aaagtgagaa agatccggtt cgatacgcag cagccattga aaaacccgtg
    11641 ggcgattgat gatcttatca gcataaagct gttcaccgag cgccagacgt ccggtccctg
    11701 gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca agatggcgaa
    11761 ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac aagataatca
    11821 ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat attcatataa
    11881 cgttgctgac gaaaaggaga catcgccaca ggcacgtacg ggcatcactg gcattaacgc
    11941 attacaaata accatctcat ctgcctgcaa cgcctcttcc agagaagctt gcacttcgac
    12001 aagctgataa gaggattgtg ccagcaaacg gatacagaat tgtcgcataa tgccgttaac
    12061 acctgcctga tccaggcgcg gcgtataaac tacgttgccc ttccgccaga acaaattagc
    12121 cgcacagcat tccgtaaccc acccttcgct gtcaaggacc agcgcctcat cagcgtttgt
    12181 ctgctcaaga tgagagcgga tcaatacttg ctcaagccga ttaagatgtt taatacctgc
    12241 aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtgatcc cttcgttacg
    12301 caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg ccggtccact
    12361 gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt tcagtacacc
    12421 attttgctgt tctgctgcca gcgttttcat ctcttgttca agttgaggcc agaagtcaca
    12481 ggaaatcatc aaccgctgac aagcatcctg tagtcgctgg atatgcgccg ataacaaact
    12541 gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact gcgttgcccg
    12601 atcgcttact gccagcgatt cctgcttata accgttaatt aagaacatac cggctcctta
    12661 tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat aaaagctaag
    12721 aaaaaaggcc cgcaagcgga ccttttataa gggtggaaaa tgacaattta gatcttttta
    12781 aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagagt gtattccatt
    12841 ccgctaacct gacgcgcttc gtgcggtacg aaatccagat cgcaaccttc atccgggtta
    12901 tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagat
    12961 tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac
    13021 acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca
    13081 gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc aatgcctgca
    13141 tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg cggtgacgtc
    13201 atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg
    13261 cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg
    13321 aagccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta
    13381 gataatgcac gtgccgcgcc aaaaccacca acacccagcg gcgtactggc tttctctgcg
    13441 ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg
    13501 ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac
    13561 atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg
    13621 atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg
    13681 ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg
    13741 ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc
    13801 ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca
    13861 aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg
    13921 gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt
    13981 ccggtcacaa ctacacgacg cttagacacg tttgtcctcc agggagggaa aaaatgattc
    14041 tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat gttcacttac
    14101 gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt ctcagcttct
    14161 tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc aacggtgtca
    14221 agagaatccg cgcccaggtc ttcaacgaaa gaagcattgt tggtaacttc ttcctgctta
    14281 acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct catactctta
    14341 aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat gttgaaaaat
    14401 ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg ccttaccccc
    14461 caataacgca aatcattttg cactaattat tactcataac cacgcagact gcgcgcaaca
    14521 tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac atgcaaagtt
    14581 tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt ggcgatttcc
    14641 tgtgcgccgc cgaggcgacc cgcaggaacc tgcgccagga tacccgcacg ctggtcatcg
    14701 ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt tacagtaata
    14761 ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc caatcaagcc cgctttcgcc
    14821 gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc gatagtgata
    14881 atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag acggaaaaca
    14941 gatgaaaggt tggtttcgat aatatcgttc cactcttcat ctttcattcg cattaacagg
    15001 ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa ttctgcgcga
    15061 attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat cagacctttg
    15121 ccgttggcac ctaaataatc actgatcgcc tgagcgccat tttcactggt cgcagtgcca
    15181 ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat tccgcggctt
    15241 gcaccggtta ccagtgcgat ttttccttca aaattcatga ttttcctctt ttaaagctcg
    15301 agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt gtcgacaatg
    15361 cgtttcgtca ggccagtaag cactttgccc gggccgactt catagagatg ttctacgcct
    15421 tgcgctgcca tgtactcaac agacttcgtc cactgaaccg ggttatacaa ctgacgtacc
    15481 agtgcgtcac gaatggcatc accattggtt tcgcatttca catcaacgtt attcacaaca
    15541 ggaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt gtcggctgct
    15601 ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagagc gcgttttgcg
    15661 cccgcagctt tacaggcagc gccagcacgc tcaaccgctt ctttatgacc ggcaataacc
    15721 acctgtcccg gagagttaaa gtttaccgga gaaacgacct gaccttctgc agcttcttca
    15781 cacgcttttc caatagacgc atcatccaga ccgatgattg ccgccatagc gcccgttcct
    15841 tccggtacgg cttcttgcat gaacttgccg cgcatctcaa ccagacgcac cgcatcagcg
    15901 aaatcaatca caccagcgca aaccagcgcg gaatattccc ccaggctgtg accggccatc
    15961 attgccggtg ttttaccgcc ctgctgctgc catacgcggt acagcgcaac agatgcagtc
    16021 aacagcgctg gctgggtttg ccaggtttta ttcagttctt cagctggccc ctgctgggtc
    16081 agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag caaacgtttc ttcgacaatt
    16141 ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg tccagggaac
    16201 acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag cgcggagccc
    16261 caagtgaatc caccgccaaa ggcttcaagc agaaccaact gccccggctt aatgcgcccg
    16321 tcgcgtacag cttcatccag cgcgcacggg acagatgccg cagaggtatt accgtggcga
    16381 tccagcgtca ccacgacatt gtccatcgac ataccgagtt ttttcgccgt tgcactgata
    16441 atacgcaggt ttgcctgatg cggaaccagc cagtccagtt gagaacggtc aagattattc
    16501 gccgccagcg tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt gaagacttcg
    16561 ttgcccgcca tcgtcagatg aattgaattc tctggattca cacggtcagc attaggcagc
    16621 gtcagcaact caccatagct accgtcggca tgcagatggg tggagatgat tcccggctct
    16681 tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa aaataataat agtcccacgg
    16741 tctgttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc atacttcacc
    16801 gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc tgcgcaggct
    16861 gctgcaacgt caaatgccgg gcaaccttta atgcccaaca tgctttgaat ctgacaagct
    16921 gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc aatctggtct
    16981 ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat ggttgaaacg
    17041 gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg tacgagtgac aatccactcg
    17101 tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc gggcagatag
    17161 ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg ttatataccg
    17221 tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc cgcttttgcc
    17281 accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg caattcgctg
    17341 aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa aagctcgctg
    17401 attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac aggcgccgtt
    17461 atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta gccaacgctt
    17521 taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca gcagagaaag
    17581 gaacatcctg acaacacctt ccatcgtctt taatgtgaca tttcctgtaa agccgtcaca
    17641 aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc cgatataatt
    17701 gatagaaggg attgttttaa gcaccgctga ggcatcccga atactatcga gaccctttac
    17761 ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca ccacctcttc
    17821 agccaggact gagcccataa tggcaaattg caccaacatt gtgctgtcac aatcgacgtt
    17881 ggcccctaag tcaaggacca ccgttttgcc cttttgctga tgtggtaata ccgtcaccag
    17941 cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc ccatcagtgc
    18001 cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca gctccagggc
    18061 cacgcgcatt gaactcccac gactggagcg gatagcttgc gaaggccggg catcactggc
    18121 gataactgac tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt cagctttagc
    18181 aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt gcgaattaga
    18241 attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc ctcccatgac
    18301 atctaacgcc agggttagac gtgtcaaggt atcgtcgccg cccggtttgg tctttcccca
    18361 gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta cttagcgatg
    18421 accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg tttttcacca
    18481 gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga acgacgcatg
    18541 ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac tcctcaatta
    18601 cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct ggctaatacg
    18661 gcaaatgggt ttggcttttg cgcttcttca ggcagttcac caaagaccat gtccgcttcg
    18721 gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag gatgatttca
    18781 tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat cggttcatac
    18841 gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca atacgttgtg
    18901 tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac cgtcaccttc
    18961 gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat ggagcattcc
    19021 acatcactgt ccacactgac tacggattcg gcgacgcgct caacctgatc aggggtatag
    19081 ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt caggggtaat
    19141 tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc aaagaaaaag
    19201 gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact gctttatttt
    19261 cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt aaaatggcta
    19321 tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta gcctccacct
    19381 cgccctggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt gcagcacccg
    19441 aggtcgacga aactccacac agcgacgaat caccgcgaca gttggtgctt cgactggcac
    19501 aagaaaaagc gcaatctctg gcgtcacgtt atccggatca tttaattatt ggttcagacc
    19561 aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa aatgcccgtc
    19621 tgcaattacg caaagccagc ggcaatatcg tcaccttcta tactggactg gcgttgttta
    19681 attcggcgaa tgggcatcta caaacagaag tggagccttt tgacgtccat ttccgtcacc
    19741 tgagcgaggc ggagattgat aattacgtgc gtaaagagca tcccctgcac tgcgcgggta
    19801 gctttaagag tgaaggattt ggcattactc tgtttgagcg tttagagggg cgtgatccta
    19861 acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg gaagggaaaa
    19921 acccgctgat gggatagttt tttgcgccgg atgcggttac ttcgcatccg gcaatcggtg
    19981 ctggcggcct gatgcggcgt aaacgcctta tcaggctaac cgcccattat caggtttata
    20041 tgcttgtatt agcgcgcgtt acgcagcttt tgcaaacaac gcttcaaacc ttcatccatc
    20101 ggcgcttcga tacgcatcac ctcaccggtc cccggatgag taaatttcaa cgctgcagcg
    20161 tgcaggaaca ggcgatttaa tcccgtgcct gcttcagtga gctgtctgtc aaattcacgg
    20221 tcaccgtagc gatcgtcaaa ggcaatcgga tgacccgcat attgtgtatg cacacggatc
    20281 tgatgagtac gccctgttac cgggctacaa cgcaccaggg tggcaaatac atagcgttct
    20341 tccactttaa agcgtgtttc cgacggtttg ccttcctgac tcacacgcac gatacgttcg
    20401 ccgctttgca gaatattttt cagtaacggt gcttgaacgc tcttcacatg cgactgccac
    20461 tgaccgcgca ccagcgccag gtaatctttt tgcatccctt tttcacgtaa ttgctcatgc
    20521 agagaacgca acgccgagcg ttttttcgct accagcaaca cacctgaggt gtcccggtca
    20581 agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa accttcaata
    20641 acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt attcagcacc
    20701 aggatgtgat catcttcata taagatgacg tccgccagcg ccgccacctt ttgcagatgt
    20761 ggcgaaaccg cctcttcttc ccgctcagca acgcgaaccg gtggaatacg cacctcatca
    20821 cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac ttcgccttta
    20881 cgcaaaatac ggtaaatcat actttttggt acgcctttca attgggtacg caaaaagtta
    20941 tcgatacgtt gccccgcttc gtcagcggtg atagcaacaa tttttacgga tggagtctct
    21001 gttttcatgg ttggcgattc taattagcca acaggattcg cgccactcat ttttctatgc
    21061 ttatatttac ttttgcacct tattacttca ctgcgtgatc actttattga tggttattaa
    21121 accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat ggtaaaagtc
    21181 atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc catccttgtt
    21241 aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac gcagcaatgg
    21301 cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc cttaccggta
    21361 gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg cgctgttttt
    21421 ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat aaccgtgagg
    21481 ttggcgacgc gactagacac gaggccatcg gttcacaccc ggaaaggcgt tactttgccc
    21541 gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa tgttaatcaa
    21601 cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc tgtatgacct
    21661 ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag gtaaaatcac
    21721 ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac gtcacggttt
    21781 cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg ctcatggtcg
    21841 tcccaacatt aaagatgtgt tgcgtgaagg tcaggaggtc attgttcaga tcgataaaga
    21901 agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg gtagctatct
    21961 ggttctgatg ccgaacaacc cgcgcgcggg tggcatttct cgccgtatcg aaggcgacga
    22021 ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg ccggaaggca tggggcttat
    22081 cgtgcgcacc gctggcgtcg gcaaatctgc tgaggcgctg caatgggatt taagcttccg
    22141 tctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggccc cgttcctgat
    22201 tcatcaggag agcaacgtaa tcgttcgcgc attccgcgat tacttacgtc aggacatcgg
    22261 cgaaatcctt atcgataacc cgaaagtgct cgaactggca cgtcagcata tcgctgcatt
    22321 aggtcgcccg gatttcagca gcaaaatcaa actgtacacc ggcgagatcc cgctgttcag
    22381 ccactaccag atcgagtcgc agatcgagtc cgccttccag cgtgaagtcc gtctgccatc
    22441 cggtggttcg attgttatcg acagcaccga agcgttaaca gccatcgaca tcaactccgc
    22501 acgcgcgacc cgcggcggcg atatcgaaga aactgcgttt aacaccaacc tcgaagccgc
    22561 cgatgagatt gctcgtcagc tgcgcctgcg tgacctaggc ggcctgattg ttatcgactt
    22621 catcgacatg acgccagtac gccaccagcg tgcggtagaa aaccgtctgc gtgaagcggt
    22681 gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct cgctttggcc tgctggaaat
    22741 gtcccgtcag cgcctgagcc catcactggg tgaatccagt catcacgttt gtccgcgttg
    22801 ttctggtact ggcaccgtgc gtgacaacga atcgctgtcg ctctctattc tgcgtctgat
    22861 cgaagaagaa gcgctgaaag agaacaccca ggaagttcac gccattgttc ctgtgccaat
    22921 cgcttcttac ctgctgaatg aaaaacgttc tgcggtaaat gccattgaaa ctcgtcagga
    22981 cggtgtgcgc tgtgtaattg tgccaaacga tcagatggaa accccgcact accacgtgct
    23041 gcgcgtgcgt aaaggggagg aaaccccaac cttaagctac atgctgccga agctgcatga
    23101 agaagcgatg gcgctaccgt ctgaagaaga gttcgctgaa cgtaagcgtc cggaacaacc
    23161 tgcgctggca acctttgcca tgcaggatgt gccgccagcg ccaacgccag ctgaacctgc
    23221 cgcgcctgtt gtagccccag caccgaaagc tgcaccggca acaccagcaa ctcctgcaca
    23281 acctgggctg ttgagccgct tcttcggcgc actgaaagcg ctgttcagcg gtggtgaaga
    23341 aaccaaaccg accgagcaac cagcaccgaa agcagaagcg aaaccggaac gtcaacagga
    23401 tcgtcgcaag cctcgtcaga acaaccgccg tgaccgtaat gagcgccgcg acacccgtag
    23461 tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc gccaggcaca
    23521 gcagcagact gccgagacgc gtgagagccg tcagcaggct gaggtaacgg aaaaagcgcg
    23581 taccgccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta acgatgataa
    23641 acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg ttcaggaaac
    23701 cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc agctcaatca
    23761 gaaagtgcgt tacgagcaaa gcgtagccga agaaacggta gtcgcaccgg tggctgaaga
    23821 tactgtcgct gccgaaccaa ttgttcagga agcgccagct ccacgcacag aactggtgaa
    23881 agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga acaatgctga
    23941 taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc acctgcgcgt
    24001 gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccaat cgccaatgcc
    24061 gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga tccgctatcc
    24121 gattgtacgt ccacaagatg tacaggttga agagcagcgc gaacaggaag aagtgcaagt
    24181 gcagccgatg gtgactgagg tccctgtcgc cgccgctgtc gaaccggttg ttagcgcgcc
    24241 agttgttgaa gaaatggctg aagtcgtaga agcccccgta ccggttgccg aaccgcaacc
    24301 ggaagtggtt gaaacgacgc atcctgaagt gattgccgcc gcggtaactg aacagccgca
    24361 ggtgattacc gagtctgatg ttgccgtagc ccaggaagtt gcagaacacg cagaaccggt
    24421 ggttgaaccg caggaagaga cggcagatat tgaagaagtt gccgaaactg ctgaggttgt
    24481 ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg aagtcgcagc
    24541 agaagttgaa acggtaaccg cggttaaacc agagatcacc gttgagcata accacgctac
    24601 cgcgccaatg acgcgcgctc cggcaccgga atatgttccg gaggcaccgc gtcacagtga
    24661 ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc atacggcaac
    24721 acatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat tagctcaagt
    24781 aatcaagccc tggtaactgc cagggctttt ttatttcatc tttgaatcaa catattaaaa
    24841 ttcatcgaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa tgcccgcccc
    24901 ggcgatccca gagcgggcag acaaaacata cccagtttca tgatatgttt taaagcgaaa
    24961 ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc
    25021 ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac
    25081 cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaatg aatccagcga
    25141 ctccagttcg ttcagctgcg tgcctaattc cgcgcgaacg gtcaggacat tgttcagcga
    25201 gtttttcagt ccgcggttgg ttttatctaa cgccgcagcg gcggtttctt tatccgcttc
    25261 gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg caaaaagatt
    25321 ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac tgtcgaaaat
    25381 tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt gcttaatact
    25441 ttctgttccg ccgacataat caccgttagc ctcgctaaac ggcgcagtct ctgttttata
    25501 accggcaaaa atgtagcgcc cgttaccgtc agtggtgttc gccagattca gcaactggtc
    25561 acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca aagtgccatt
    25621 gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac
    25681 actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt
    25741 ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtcagaagg
    25801 gttaacgact cgcttacctg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga
    25861 attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc
    25921 tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc
    25981 gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc catactcctc atcgagattg
    26041 acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg
    26101 ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg
    26161 tcgttaaagg atttcgctcc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc
    26221 tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc tattttcgct
    26281 tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag
    26341 ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta
    26401 ccgttggcat ctggtgtcac cgtaaaagtg gtattgctgg caaggcgggt gacctgccac
    26461 tgattattat cgaacgagat tttgtaatct gtcgccagta ccgcggaggc atcagttacc
    26521 gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg
    26581 atagcaaaga aatcttcacc ggcatcgccg ttggcatcaa atccggcttt gtgttggctg
    26641 ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt cttgtccaga
    26701 tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagcaa tttctccggg
    26761 atctcaatat tgcctgccgt cccatcaaca taagcgacag tcgtacgaga agggtcggcg
    26821 ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg
    26881 gccatcgtga tgttataagt gccgccatcc tgaacgctga cttctacacc aacaatctgg
    26941 tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc
    27001 cccacgcctg tcagacgtga gatttgatcg ttcaggctgg caatttgttt agcgtaattg
    27061 ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgaaga
    27121 tactgatcgg tggttttaaa ctgattcacc accccttctg atttcccaat cagcgcctgg
    27181 cgcgctgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc
    27241 atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc
    27301 tcatagcggg cagtcaggcc gctactttgc gtctgcgccg cacgtaattg gttggtaata
    27361 aacgcatcat actcacgctg cacaccagaa acgtagacac cattaccaac ccagccgcca
    27421 gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg
    27481 ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg
    27541 ctcatggcat tattgatcaa gctggacatg gaggttcctt ataagccttc aattacagag
    27601 tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc
    27661 tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctgaatcatg ttggtgagtt
    27721 tacgggcata gtgaggatcg gtggcatagc ccgcgtcctg tagggcctgc gccccctgtt
    27781 ccgcactcgc ggcggtcgtc acggcagcgt agcgcgggtt acgcgttaac agcccaacgt
    27841 aatccgacaa tgcttccaga tacgagctgt agacccgaaa tttcgctttt actatcttcg
    27901 cttcgccatt ttcatattca gtcgtggtga tttcagtaac tggccctttc cagttgccag
    27961 aggctttgac gccaaacagg ttatagctcg gctcgccgtt ttcgcggcgg atttgccgtt
    28021 gcccccaacc agattccagc gccgcctgag cgaggatcaa atgatgtggc acaccgcttt
    28081 gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgcttta ctgtcacccg
    28141 gcagcgaatc atcgtagtta cgtggcacgg ctttttgcac cagctgcgaa agcgcctggt
    28201 tttgataacg caccacggtt tcgagcggga atttcatcgg tgctgctgac gtgggctcct
    28261 ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc cccagacctt
    28321 tgcccgccgt catctgttgg gcaatctgct ggtcatacat actggtatac aggcgagtgt
    28381 gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc aacatcatct
    28441 gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc gggtcttcgc
    28501 ccgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt
    28561 tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat
    28621 tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga gcgcgcgcac
    28681 cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa
    28741 atcgatctgc gtttgtggag taaccacagt ctgtccacca ccaaacggtg tatctggctg
    28801 gctgacattg gcctgacgat tgactgtaac tgagagattc ccctgcgcta ccgcgcagct
    28861 gtcgagggtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt
    28921 agcgtcctgc ggggtgacat taacctgcat attctggata tcggcaagga aacggacctg
    28981 ggaactgttg ccactcggta cgcgcacctg aatagtccgc gcatctaacg cggtggcgct
    29041 gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc
    29101 gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca attcacgttc
    29161 aataaccgca ccattggtga tccgtccacc gttcagttgg ttcacctgaa cactgctacc
    29221 gccagcggag gctcctgcgc cgccaaccag aatattgccc tgtgccagcg catacacctg
    29281 actgtcaacg cccttaagcg gtgtcatcaa caacgtacct ccacgcaagc ttttggcatt
    29341 acccatggaa gaaaccacca catcgatggt ttgcccctga cgtccaaacg gaggaagtga
    29401 cgctgtcacc attaccgcag cgacgttttt tagctgcata ttggtgcccg tcggaacggt
    29461 aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt
    29521 ctggtcaccg gtgccatcca gccccaccac cagaccatag ccaatcagtg agttttgcct
    29581 taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtaaccag
    29641 tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg
    29701 ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct
    29761 acgtattcaa tgcgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata
    29821 gtgcgtggat taaccgcgcc agagaagcga ataaattcgg taccctgatt aatggcaatc
    29881 tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac cgtcaccgtc
    29941 aacgtgccgc taaaggtatt gctggcattg gccccgccct ttccgttgaa cgtgttacca
    30001 ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca
    30061 gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg
    30121 gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga
    30181 tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg
    30241 ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg caccagcggc
    30301 gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga aatggcataa
    30361 gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcaataaac cggtgaacct
    30421 gccaccggtt atgccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac
    30481 cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc
    30541 cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc
    30601 cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt tttcgccaat
    30661 gctctccagc ccagtgtcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg
    30721 agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa
    30781 cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg tcaccagctg
    30841 cccgttctga tccacctgga aagagccgtc acgggtatag gctgatgaac catctggcaa
    30901 catcacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttgt tggtctgcga
    30961 caggttgccc tggctgtgta aacgttcagt ggcgaccggg cgtacccccg tgccgatttg
    31021 taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg
    31081 ataaagcaga tcttcaaaca ccgcacgctg acgcttaaaa ccgttggtac tgacgtttgc
    31141 caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat
    31201 ccataatgaa ctgatcataa aatgtcctgt agtggattag tgacaaaatg catatgtttt
    31261 cttgccggat gcggcgtaaa cgccttatcc ggcctacaag agctcgcaaa atcgataaat
    31321 tgcgattgcc cccatagacc cgataagctt gcgcatcggg caacttatac ttgtcatgta
    31381 tcctttcaat taactcatcg acagcagttg gttggcacgg cctgcgttat catcgacgct
    31441 gctgatcacc ttcatctgca tttcaaaacg ccgcgcgctg gcaatcatgt cgctcattgc
    31501 cgcaacggca ttaacgttac tgccttccag aacccccgac atcacacgca aggttggatc
    31561 tgcctgcagt accggcccac gcgtggcctg tctttctgcg cttaaacgaa aaatgccgtc
    31621 gtcaccgcgc tgcacttcgc tgcccgtggc tttcaccagc ttcagacgcc ccactggcgc
    31681 aaccgtattt gccggatcgc ccggattgag cgccgagatt gtgccatcgg cagcaatcgt
    31741 gatttccgcc ccttccggca cagcaattgg tcccgcctcg cctatcaccg gatgcccctg
    31801 aattgtcagt tgtccggtgg gatcaacctg aatgctgcca ttacgcgtat acccttcgct
    31861 gccgtcagcg gtctgcacgg ccagccagcc atcctgctgc aacgcaacgt ccagcgggcg
    31921 cgaggtgtaa tccattttgc cgggcgtcat gtctgcaccc ggtgttgacg ccgttaccaa
    31981 cgtgcgcgta ggcagtgaaa gcccttccac cggcactgcg cgcaaggcgt ttaactgcgc
    32041 acgaaaaccg ggcgtagagg cattggccag attactggcg gttaccgcct gttgattcag
    32101 cgtctgactg gctgctccca tcgcggtata aattgcgtga tccattgagc tatcccgtca
    32161 gcgattagcg taagttaacc agcgtgttga ggatctggtc ctgggttttg atggtctggg
    32221 cgttagactg atagttacgc tgggcaacga tcatattgac cagttcttta ctgagatcga
    32281 cgttggacgc ttccagcgca ccgttggtca gggtgccaaa gttgcccgtc ccggctgtcc
    32341 ccaacagcgc cacgccagaa gattgcgtcg cagaccagac gttgtcgcct tcggatgcca
    32401 gaccttcgtt gttggcaaag ttcgccagta caatctgccc cagcagttgg gtttgttcgt
    32461 tggaatagtt gccaacaacc gtaccgtcat cattgatttg ataactcacc agatcgcccg
    32521 gtttgtatcc gttctgggtg gttgccacaa tgttgttagc gccggtattt tgctgcatgg
    32581 agttgaggaa gctcaggcta aacgtggcgg gatctgcgcc gttaattgcg ccagtggtaa
    32641 tattctctgt tggatttgag gtcagaacgc cattggaatt aaacaccagc gtcattgcat
    32701 ggtcagctgt gcctgttgga tcactgctat cctgggtgta gacctcccag ttattatccc
    32761 cggtcttcac aaagtacacg ctcatgtcat gagcattacc ctgactatcg aaaacagtca
    32821 ccgaaccttt tttgttatag ctatccgcat tgctggcatc aaatgcgtta acagtaggaa
    32881 gcggatcact ggaattcagg ttgatctgca tcgacgccgt ggtggtagtt ttcgctgcca
    32941 tcagggtatt cgggatcgaa atattggtcg gattcgcccc ttgctgaata gtcggcggcg
    33001 taccggttgc cgggtaaccc gtcagctgta aaccttgcat attcaccagg ttacggtttt
    33061 catccagctt aaattgtccg ttacggctgt agaacaccga accgttgctg tctaccagac
    33121 ggaaaaaacc gttctggctg atagcaacgt ccagaccacg cccggtgttg gtggtcgtgc
    33181 catcggtaaa gtcctgagtg ataccggcaa cttttacccc cagtcccact ttcgaaccgg
    33241 caaacatatc ggcaaaagag gccgtgcctg atttaaagcc gtatgtggcg gagttggcga
    33301 tattgttgcc aataacatcg aggttggtgg cggcagcgtt taatccgcta accgcttgag
    33361 aaaaggccat gactgactcc tgaaatgtga aggcttaaat tatctgccgt acttcgtcga
    33421 gggtggtggt gccgtaagtg ccgagatcca gcgtattacc gttgttgccg cggatcacac
    33481 cctgcaccag agcaaactgc agcggctggg caaccagttg tgtaccaccg ttactggcgc
    33541 taatcgctac gttgtaagaa ccgttcggcg cagtgctgcc gtcagtcaac gtaccgtccc
    33601 aggtgaaact gtggactccg gcggtcagtt caccaatatc aatggtgcgc acaaccgcgc
    33661 catttttatc ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc tcaacaccaa
    33721 acggcgtggt cgtggtcaca gccccttctt cactgccggt tcccgcaaga acagtggtgc
    33781 cggggatcat cacgccgtga ccgatcaggt tactggcctg taacgactgg ctgttatcaa
    33841 tctgtccgga aatagatccg agcgtggtat tgagtttttc aatcccgctg accgtgctga
    33901 tttgtgccaa ttgcgacgtc agctcgttgt tttccattgg attggtcggg tcctggtttt
    33961 tcagctgcgc caccaacaaa gtcagaaaac tgctttgtaa atctgcggcg ttgctgcccg
    34021 tgagcgaact actgctggtg gtactgacgc cggtatttgt cggatcggtg gtggttaccg
    34081 caatggacat agctttctcc tttattgacc gagcgtaagg gttttcagca tcatgctttt
    34141 caccgtgttg agcacttcaa cattggcctg atagctgcgt gacgccgaca tggtgttaac
    34201 catctctccg acaacatcaa cgttcggcat ttttacgtag ccctttgcat ccgccagcgg
    34261 attacccggt tcataaacca gtttgtccgg ggcctgactt tctatgacat cggcaacctt
    34321 tacgccgcct gtagcagcac ctggtgcagc gttaacctgg aataccacct gttttgcccg
    34381 atatggctgt ccatcaggac cggtcacgct atcagcattc gccagattac tggccgccac
    34441 gttcaggcgc tgggactggg cagttaacgc cgacccggcg atatcaaaaa tattcagcag
    34501 tgccatccgt taatttccgc tctgtaaaac gttcatcatg cctttgattt gcccgctcaa
    34561 cgcgctaagg ctcatctggt attgcaggct gttatcggca aactgggtgc gttcgcgatc
    34621 catatcgacg gtattaccgt caagcgaagg ctggtccgga atacggtatt gcagttctgc
    34681 ggtaggaggc gtcagcgcct gcgccggaat gtgttgcgtt gaggtcatcg tcagagcaac
    34741 cacactggtt gcatcccgtc cacgttgcat gactttttta agttcactgg caaaatcgat
    34801 atcgcgcgcc tgataaccag gggtatcggc attggcgatg tttgctgcca gcacttcctg
    34861 acgctgggcg cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt cgagcttatc
    34921 gagcatatct cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc tttacgcgcg
    34981 cgttatcggc ggaataaacg caaaatgggt cgctatttat gccgttgatg gtcattgcgg
    35041 acaggtacaa ttcacgttgt agaaatggct gggggcgaaa atgctggcaa taaaacgtag
    35101 cgtggcgatc atcgcgatac tgttcagtcc gttaagtgcg gcgagcaatc tcacatcgca
    35161 attgcaaacc ttttttagct cccagctcgc agggataagt gatgaggttc gtgtttctat
    35221 tcgtacagcg cccaatctac taccgccgtg cgagcagcca ttgctttcga tgagcaataa
    35281 ttcccgcctg tggggcaatg tgaatgtgtt ggcacgctgc ggtaacgaca aacgatattt
    35341 acaggttaat gtgcaggcca caggaaatta tgtggttgcc gcgatgccca ttgtgcgggg
    35401 aggaaagctg gaagctggca atgtcaaact gaaacgcgga cggctggata ccctgccgcc
    35461 gcgtacggtg ctggatatca atcaacttgt tgatgccgtt agcctgcgcg atctatcacc
    35521 cgatcaacct atccagttaa cccagtttcg ccaggcatgg cgggtaaaag cgggacaacg
    35581 cgtcaacgtg atcgccagcg gtgatgggtt tagcgccaac gcagaaggtc aggcgctgaa
    35641 caatgcagcc gtcgcacaga atgcacgagt gcgcatggta actggacagg tagtcagcgg
    35701 cgttgttgat gcagatggga atattcttat aaacctgtaa gctgttaaag attacccgtc
    35761 ccttgccgat aaataagcaa cacatgataa aagcgccctc aatgaggaat aaaccatgag
    35821 tattgatcgt acttcgcctc tgaagcccgt aagcaccgtt caaccgcgcg aaaccactga
    35881 cgcgccggta acgaacaccc gggcggcaaa aacaacagca tccaccagca ccagtgtgac
    35941 gttaagcgac gcgcaagcaa aactgatgca acccggcagc agtgatatca atcttgaacg
    36001 tgtcgaagcg ttaaaactgg cgattcgtaa cggtgaacta aaaatggaca ccggcaaaat
    36061 tgccgatgcg ctgatcaacg aagcacagca agacttgcag agtaactgat catatgacgc
    36121 gtcttgcaga gatcctcgac cagatgtccg ctgtgcttaa cgatctcaaa acggtaatgg
    36181 atcaagagca gcaacatctc tctatggggc agatcaacgg cagccagttg caatggatta
    36241 cagaacaaaa aagctcactg ctggcaacgc tggattacct cgaacagtta cgcaggaaag
    36301 aacccaatac agcaaatagc gttgatatta gtcaacgctg gcaggaaatt gctgtgaaaa
    36361 cgcagcaact acgccaaatg aatcaacata acggctggtt actggaagga cagattgagc
    36421 gcaatcaaca ggcgctggaa atgttgaaac cgcatcagga accgacgcta tatggggcga
    36481 acggtcagac ctcaacaacc catcgcggca gtaaaaagat ttcgatctga agattatctc
    36541 cggcctgcac tgcaggccgg aatgcattgt tacaccgtcc ggcgggcaaa ttctttaact
    36601 ttgaagccca gtaccgccag tgcagcgaag tacgcggcaa tccccgccag cacgaccgcc
    36661 attaaacgca gcaagcgcca gggcatggta cccaatgacc actccggcat gatatgtaac
    36721 atacctaaaa gcacgccaga catcaccagt accgccacca ccagacgcaa cagaaacgcc
    36781 atccagccag gttgcggggt aaagattttc tgcttacgca actgccagta aagcagtgaa
    36841 gcattcagac acgccgccag accaatagaa agtgacagcc cggcatgttt caacggaccg
    36901 ataaacgcca ggttcatcaa ttgcgttaaa atcagcgtaa cgatggcaat tttcactggc
    36961 gttttaatgt cctggcggga ataaaagcca ggagccaaca ctttcactac aatcaggcca
    37021 atcaaaccca ccgagtaggc aattaacgcc cgctgggtca tcagcgcatc aaaggcggta
    37081 aatttaccgt actggaacag cgaaacggtt aacggaccgg aaagaatgcc caacgcaacc
    37141 gcactcggca gcgccaacag gaaacaaaga cgcagtcccc agtccatcaa acggttgtat
    37201 tcatcatgat tgccactggc aaaacttttc gacagcgacg gtagcaaaat ggtgccaagc
    37261 gccacgccca gcacaccgga cggaaactcc attaagcggt cggcgtaata catccatgac
    37321 accgaaccgg aagcaagaaa cgaggcaaaa atggtgttga tgattaagga gatctggctc
    37381 acagagacgc caaggattgc cggtcccatc tgtttcacca cacgcattgc tccggcatcg
    37441 tggaagttaa tgcgtggcaa aaccagcatg ccgatcttct tcagatgcgg tagctgatac
    37501 accagctgca ggatgccgcc gaccgtaaca gcccacgcca gcgccagcac cggtgggtta
    37561 aagtacggtg cggcaaacag cgcgaaacca atcatgctga tgttaagcag tgttggagca
    37621 aacgccggaa tcgagaagcg gttccacgta ttcagaatcg ctcccaccag cgacgccagg
    37681 gagatcagca agatataggg aaaggtaatc tttagtagct ggctggtcag ggcaaatttg
    37741 tcagctgtgt cagcgaagcc tggcgcggtc accatgatca cccacggtgc ggcgagcatg
    37801 ccagcgaccg tcacaaccgc cagcgcaagt gtcagcaggc cagaaacata agagacaaag
    37861 acccgcgtgg cgtcttcacc ctgcttactt ttatattccg ccagaatcgg tacaaatgcc
    37921 tgggaaaatg ccccttcggc aaagatacgg cgtaacaagt taggaagttt aaaagcgacg
    37981 aaaaaggcgt cggttgccat ccctgcgcca aaaattctgg cgacaattgc gtcacgtgcg
    38041 aagccaagca cacgcgaaaa catggtcatc gagctgacgg cggccagcga ttttaataaa
    38101 ttcatcggtg ttctaatcca gacccacggg caaaacgcct gcaaggcagg cgctgaaaga
    38161 ggcgaatagt ctacctggat tatggtgaat tgctaccgcc agatgttaca gggtttattc
    38221 actcatcgca tcgcgccaga tcttgtcaac gatacgttgc gccagcactg cctgttcgcc
    38281 ggcggtttgc ggaactgtct gattttgcac gcattcaatg aagtgccgcg cacagccgac
    38341 aaatccacgt tgctcaagcg tgctctgcca accaggaatc ggtttattca ctacaccctg
    38401 cccacgctcc tcacgccatt cgcgcatttc cgtaatgtcg atgagcgcac cgtcagtcac
    38461 ggcctgcacg gtttcacgct gacttccggc acggcgatgc atacaagtgg tgatctgcaa
    38521 aggaccggcc gaaaaatggt gctcggcaaa cagcatttcg ccagcgtcgt tagtcagtag
    38581 cgtaccgcca tccagagagg ctttgccgcc cgacaaccac agcgcggtat ccaccacatg
    38641 cagataatca tccagcaaag tgaaataaag atcgtgtggc ccgacgctat tgctacgatg
    38701 tttatccatt cttagcgagg ctgcggtggc gagttgcgtt tttaactcac cgtagagtgg
    38761 tgcgaaacga cggttaaaac cgaccatcaa cgtcagtttt ttccgcgccg ccagttccac
    38821 cagccgttca gcatcgcgca gattttctgc cagcggttta tcgacacaga catgtacccc
    38881 cgcattgagt aacgtactga ccacgtcaaa gtgactggcg gtgctggaat gtacaaaaac
    38941 cgcatcgcaa ctggcagcaa ggctggagaa cgaatcggca taaggaatgc gccagctttc
    39001 acaaattggc agggctttcg cgcgcgtagg cgaccaggct ccttgtaacg tccagtcaga
    39061 cgctgccgcc agcaccggta accacgcttt ttgagcaatg ccgcctaatc ccactacgcc
    39121 gatacgtaat tttttcactt taatctccca ggtgggccag caacgaatca agacgctgtt
    39181 tcagttctgc cacttcgatt tccagggctt cgacgcgggc ctgtaaatca gcgtcaaccg
    39241 cgctcgacat atccgttacc gacggctgat cctctacctc accgctgaaa agatgcatgt
    39301 agcggctttc gcgtttaccc ggttcgcggg ccagacgcac cacaaaagga ccatcttcgc
    39361 gattcgccag ttgttccagc gtcaactcca cttccgccat atcgctgaat tcatacattc
    39421 gcgcggcgcg gctgcgcagt tcacccggcg tctgggcacc acgcaataac aacgtggtga
    39481 ttaacgccac ttctgcggcg ctcagtttca gatcgccaaa ttctgaatta caaaaacgtt
    39541 gctcatattt ggtgacccga ttaccaaaac cgctcaccgt gcgtagataa tgacgtttga
    39601 ccagattatc cagctgttcc tgcacttccg attcactcag attcatgacc ggttcacggt
    39661 tcgttttctg attacaggcc gttactacac cattgactga gagcgggtat tgctccggcg
    39721 tggtcacctg cttttccagc aagcagccaa tcacgcgggc ttccagagca gtaagttgat
    39781 atttcatgag attctcctta gcggccgggc gtccagtctg gggttgttaa tgccgtcagt
    39841 acgtgatcgc gccattgtcc atcaatcaac agatagtctt tcgcatagcc ttctttttca
    39901 aaacccagtc gcgccagtaa atcaccgctg cgtttattgt gcggcatata attagccata
    39961 atgcgatgaa tatgttgggt gcgctgcata taacgaatgg ctgcggtcag ggcttcaaac
    40021 atgagtcctt tgccctgcca tttttgccca atcgaataac cgagatagca ggcatgaaaa
    40081 gagccgcgaa caacattgga aaaattggca acgccaataa tctctttttc gtccgggtcg
    40141 aataagccaa agtagaaagc tgaaccttgt ttatgaaatt cgttaatcat ccccagcctg
    40201 gcctgccagc ctgatggata acagtggctt tcgtcgcgca ctggctccca gggcttgagg
    40261 aaatggcgat tctctgcgta ataatccgca agacgccagg catcacgatc atgcaccaga
    40321 cgcacgacca gtcggtctgt ggttaagcgc acttttggca cgttactgcg atagccaaac
    40381 attctatacc tactccttcc cgtaacgtct ttaatacgct gtaagtatta ctatacctgc
    40441 gcaaccagac cctttgaaaa gtccaaaatg ccattttctg cattattcac atatttgata
    40501 aaaactctct atcaaagcga cgttttgata aaaaaatatt gtccccgaag tgatgggaaa
    40561 agtcttagct gcaatgcaga atagaaagtg ctcatctttt atttcccggg aggggaaatg
    40621 tcctgcgtgt cgcaggcgag gaacctgggt aaatatttcc tgctcatcga taatatgctg
    40681 gtcgtgctgg ggttctttgt tgtcttcccg ctgatctcta tccgcttcgt tgatcaaatg
    40741 ggctgggccg ccgtcatggt cggtattgct ctcggtctac gccagtttat tcagcaaggt
    40801 ctgggtattt tcggcggcgc aattgccgac cgctttggtg ccaaaccgat gattgttacc
    40861 ggtatgctga tgcgcgccgc cggattcgcc acaatgggta tcgcccacga accgtggcta
    40921 ttgtggtttt catgcctgct ctcgggactc ggcggcacgt tgtttgatcc gccgcgttcg
    40981 gcgctggtgg tgaaactaat ccgtccacaa cagcgtggtc gttttttctc gctgttgatg
    41041 atgcaggaca gtgccggtgc ggtcattggc gcattgttgg ggagctggct gttgcaatac
    41101 gactttcgcc tggtctgcgc cacaggggca gttctatttg tgctgtgtgc ggcgttcaat
    41161 gcgtggttgt taccagcatg gaaactctcc accgtacgca cgcccgttcg cgaaggcatg
    41221 acccgcgtga tgcgtgacaa gcgttttgtc acctatgttc tgacgctggc gggttactac
    41281 atgctggctg tacaagtgat gctgatgctg ccaattatgg tcaacgacgt ggctggcgcg
    41341 ccctctgccg ttaaatggat gtatgccatt gaagcgtgtc tgtcgttaac gttgctctac
    41401 cctatcgccc gctggagtga aaagcatttt cgtctggaac accggttgat ggctgggctg
    41461 ttgataatgt cattaagcat gatgccggtg ggcatggtca gcggcctgca acaacttttc
    41521 accctgattt gtctgtttta tatcgggtcg atcattgccg agcctgcgcg tgaaacctta
    41581 agtgcttcgc tggcggacgc aagagctcgc ggcagctata tggggtttag ccgtctgggt
    41641 ctggcgattg gtggcgctat tggttatatc ggtggcggct ggctgtttga cctgggcaaa
    41701 tcggcgcacc aaccagagct tccgtggatg atgctgggca ttattggcat cttcactttc
    41761 cttgcgctgg gctggcagtt tagccagaaa cgcgccgcgc gtcgtttgct ggaacgcgac
    41821 gcctgatttg atactccccc ctgactcctc catactgaaa gtgaactgac agccaaatgg
    41881 aggagtcacg tgaagctata catttacgat cactgccctt actgcctcaa agcccgcatg
    41941 attttcggcc tgaaaaatat ccccgtcgaa ttacatgttc tgctcaacga cgacgcagaa
    42001 acacccaccc ggatggtcgg tcaaaaacag gttcccattc tgcaaaaaga tgacagccgc
    42061 tatatgccag aaagcatgga tatcgttcac tatgtcgata aactcgacgg caaaccgtta
    42121 ctgaccggca aacgttcccc tgcaattgaa gagtggctgc gcaaggtcaa tggctacgcc
    42181 aacaaactgc tgttgccgcg ttttgccaaa tcggcatttg atgagttttc tactcccgcc
    42241 gcgcgcaaat atttcgtcga caagaaagag gccagcgcgg gtaattttgc cgacctgctg
    42301 gcccactctg acggtctgat taagaatgtc agcgatgatt tacgtgcgct ggacaaactg
    42361 atcgtcaaac cgaacgccgt gaatggccaa ctttcggaag atgatattca gctatttcca
    42421 ctactgcgta atctgacgct ggtagccgga atcaactggc caagccgcgt tgctgattac
    42481 cgcgataata tggcgaaaca gacacaaatc aatttgttat catcaatggc gatttaatcc
    42541 tgacccgccg cggcagcctg tattcgcggg ttgccgccgt tttcagtttt ttctgctggc
    42601 gagtttatcg ctggtgacac atgctgcgtt gtgtcaggat attgacgcag atttcatgtc
    42661 gaggactacc atgaacaagt ttttatttgc cgctgcattg atcgtcagtg gcctgctcgt
    42721 tggctgtaat caactcaccc aatacaccat caccgaacaa gaaattaacc agtcgcttgc
    42781 gaaacataat aatttctcaa aagatatcgg tttacccggt gtggctgacg cccatattgt
    42841 tctgacaaac ctgaccagcc aaattggtcg cgaagagccg aataaggtta ccctaaccgg
    42901 agacgccaat ctggacatga actccctgtt cggtagccag aaagcgacca tgaaactgaa
    42961 gctgaaagcg ctgcccgtgt ttgataaaga aaaaggcgcg atcttcctga aagagatgga
    43021 ggtggtcgat gcgacggtac aaccggaaaa aatgcaaacg gtaatgcaaa cgttgcttcc
    43081 ctatctgaac caggcattac gcaattactt taaccagcaa cctgcttacg tcctgcgcga
    43141 agatggcagc cagggcgaag caatggcgaa aaaactggcg aaaggcattg aagtgaagcc
    43201 aggcgaaatt gtcattccat ttactgatta atcacgaggg cgcattcgcg ccctttattt
    43261 ttcgtgcaaa ggaaaacgtt tccgcttatc ctttgtgtcc ggcaaaaaca tcccttcagc
    43321 cggagcatag agattaatga ctgcaccatc ccaggtatta aagatccgcc gcccagacga
    43381 ctggcacctt cacctccgcg atggcgacat gttaaaaact gtcgtgccgt ataccagcga
    43441 aatttatgga cgggctatcg taatgcccaa tctggctccg cccgtgacca ccgttgaggc
    43501 tgccgtggcg tatcgccaac gtattcttga cgccgtacct gccgggcacg atttcacccc
    43561 attgatgacc tgttatttaa cagattcgct ggatcctaat gagctggagc gcggatttaa
    43621 cgaaggcgtg ttcaccgctg caaaacttta cccggcaaac gcaaccacta actccagcca
    43681 cggcgtgacg tcaattgacg caatcatgcc ggtacttgag cgcatggaaa aaatcggtat
    43741 gccgctactg gtgcatggtg aagtgacaca tgcagatatc gacatttttg atcgtgaagc
    43801 gcgctttata gaaagcgtga tggaaccgct gcgccagcgc ctgactgcgc tgaaagtcgt
    43861 ttttgaacac atcaccacca aagacgcagc cgactatgtc cgtgacggaa atgaacggct
    43921 ggctgctacc atcactccgc aacatctgat gtttaaccgc aaccatatgc tggttggtgg
    43981 cgtgcgtccg cacctgtatt gtctacccat cctcaaacgc aatattcacc aacaggcact
    44041 gcgtgaactg gtcgccagcg gttttaatcg agtattcctc ggtacggatt ctgcgccaca
    44101 tgcacgtcat cgcaaagaga gcacctgcgg ctgcgcgggc tgcttcaacg ccccaaccgc
    44161 gctgggaagt tacgccaccg tctttgaaga gatgaatgct ctgcagcact ttgaagcatt
    44221 ctgttctgta aacggcccgc agttctatgg gttaccggtc aacgacacat tcatcgaact
    44281 ggtatgtgaa gagcatcagg ttgctgaaag catcgcactg actgatgaca cgctggtgcc
    44341 attcctcgcc ggggaaacgg tacgctggtc tgttaaacaa taaaaatcgt agcttcctgt
    44401 tgtcattagg ttattttacc tgtataaata accagtatat tcaacagggg gctattatgc
    44461 gaattgaagt caccatagcg aaaacttctc cattgccagc tggggctatt gacgccctgg
    44521 ctggcgaact ttcccgccgt attcagtatg cgtttcctga taatgaaggc cacgtatcgg
    44581 tacgttacgc cgcagcgaat aatttatcgg ttattggcgc gacaaaagaa gataaacagc
    44641 gcattagcga aattctccag gaaacgtggg aaagcgccga tgactggttt gtcagcgaat
    44701 aatatgcagt gatttttgtt tttgccgggt cgccccggct tttttatttt acttacaaat
    44761 tttgagtaca aaatttctac acttcctcaa aataattgca caatatgcta aaaaaatatg
    44821 aatgacatgc tgttttattg ttcatataaa tcttaaaagc agaaatttat ctgtttcccc
    44881 ctttaaaaat tcggtgaata cccttactta ttggtatact gaagttgctt cagataaaaa
    44941 cgcattgaac ctcgaataac gttgtctagt aacacgaatt agggggccat gatggaaaaa
    45001 aataatgaag tcattcagac tcatccgctc gtagggtggg acatcagcac cgttgatagc
    45061 tatgatgcgc tgatgttgcg tttgcactac cagaccccaa ataagtccga gcaggaaggg
    45121 actgaagttg gtcagacgct ctggttaacc actgatgttg ccagacaatt tatttcgata
    45181 ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc cggtaaacga gtatcggcgt
    45241 cattaacatg acatgaccat ccacccaaaa ggcacctcac cggtgccttt accatttctg
    45301 ggttgtatat ctgcattcag ttaattactc tgcttaaacc atgagagcag agagaataaa
    45361 atgaaatacg atctcatcat tattggcagc ggttccgtag gcgctgccgc cgggtattat
    45421 gcaacccgcg ccggtttaaa cgtgcttatg accgacgccc atatgccacc acatcaacac
    45481 ggcagccacc acggcgatac gcgattaatt cgccatgctt atggtgaagg cgtaaagtat
    45541 gtcccgctgg tcctccgcgc acaaacgctg tgggatgacc tctcccgcca caacgaagat
    45601 gatcccattt ttgtacgctc tggtgtcatt aaccttggcc cggctgactc cgcatttctc
    45661 gccaacgtcg cccacagcgc cgaacagtgg caactcaacg ttgaaaagct cgatgcgcaa
    45721 gggattatgg cccgctggcc agaaatacgc gtcccggaca actacatcgg cttatttgag
    45781 actgattccg gttttttgcg cagcgaactg gcgattaaaa cctggatcca actggcgaag
    45841 gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg caattcgtca tgacgatgat
    45901 ggcgtaacta ttgaaacggc tgacggtgag tatcaggcga aaaaagcgat tgtctgcgcg
    45961 ggaacatggg taaaagacct gctcccggag ctgcctgtcc agcccgtacg taaagtattt
    46021 gcctggtatc aggccgatgg ccgctatagc gtgaagaata aattcccggc gtttaccggt
    46081 gaactgccca atggcgatca atattatggt tttccggcag aaaacgacgc gttgaagatt
    46141 ggcaaacata acggaggcca ggttatccat tcagcggatg aacgtgttcc gtttgcggaa
    46201 gtggtcagcg atggttcgga agccttcccg ttcttgcgaa atgtattgcc gggtatcggt
    46261 tgctgcctgt acggcgctgc ctgcacctat gataattcgc ctgacgaaga ttttattatc
    46321 gataccctac ccgaccacga taatacactg ctcattaccg gcctgagtgg gcacggtttt
    46381 aaatttgcgt cagttttagg ggaaatagct gccgattttg cgcaaaacaa aaaaagcgat
    46441 tttgatttga cgccattcag gctttcccgc ttccaataat catcattacg gcctccagaa
    46501 tgaggccgtt tttacaggag tatttaatgc gtcggctgtt gcactatctc atcaagaata
    46561 ttcgcgagca tctgatgctt tatctttttc tctggggatt gttggcaatt atggatctga
    46621 tctatgtgtt ttatttttag ttttccgcac tgacataaac tgacaaatat ttaatctccc
    46681 tttcatttag cgtgaaaaat tgaatgagat tattcctgct tctacagcac tcaccagaca
    46741 tctctgatgc tgaacccact aaacttatga a
//
LOCUS       DAZFFG010000022        44738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFFG010000022 DAZFFG010000000
VERSION     DAZFFG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.022"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            276..1592
                     /gene="fucP"
                     /locus_tag="ACHJ7J_001617"
     CDS             276..1592
                     /gene="fucP"
                     /locus_tag="ACHJ7J_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO4999869.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1625..3400
                     /gene="fucI"
                     /locus_tag="ACHJ7J_001618"
     CDS             1625..3400
                     /gene="fucI"
                     /locus_tag="ACHJ7J_001618"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO4999870.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3509..4927
                     /gene="fucK"
                     /locus_tag="ACHJ7J_001619"
     CDS             3509..4927
                     /gene="fucK"
                     /locus_tag="ACHJ7J_001619"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4999871.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4929..5351
                     /gene="fucU"
                     /locus_tag="ACHJ7J_001620"
     CDS             4929..5351
                     /gene="fucU"
                     /locus_tag="ACHJ7J_001620"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO4999872.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5409..6140
                     /gene="fucR"
                     /locus_tag="ACHJ7J_001621"
     CDS             5409..6140
                     /gene="fucR"
                     /locus_tag="ACHJ7J_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO4999873.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(6184..7284)
                     /gene="rlmM"
                     /locus_tag="ACHJ7J_001622"
     CDS             complement(6184..7284)
                     /gene="rlmM"
                     /locus_tag="ACHJ7J_001622"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO4999874.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7277..7672)
                     /gene="ygdD"
                     /locus_tag="ACHJ7J_001623"
     CDS             complement(7277..7672)
                     /gene="ygdD"
                     /locus_tag="ACHJ7J_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO4999875.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7691..8608)
                     /gene="gcvA"
                     /locus_tag="ACHJ7J_001624"
     CDS             complement(7691..8608)
                     /gene="gcvA"
                     /locus_tag="ACHJ7J_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO4999876.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(8959..9186)
                     /gene="ygdI"
                     /locus_tag="ACHJ7J_001625"
     CDS             complement(8959..9186)
                     /gene="ygdI"
                     /locus_tag="ACHJ7J_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO4999877.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9378..10583
                     /gene="csdA"
                     /locus_tag="ACHJ7J_001626"
     CDS             9378..10583
                     /gene="csdA"
                     /locus_tag="ACHJ7J_001626"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO4999878.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESVNMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10583..11026
                     /gene="csdE"
                     /locus_tag="ACHJ7J_001627"
     CDS             10583..11026
                     /gene="csdE"
                     /locus_tag="ACHJ7J_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO4999879.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11077..11883)
                     /gene="tcdA"
                     /locus_tag="ACHJ7J_001628"
     CDS             complement(11077..11883)
                     /gene="tcdA"
                     /locus_tag="ACHJ7J_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO4999880.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYYRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMIAKAARQG"
     gene            complement(12122..13219)
                     /gene="mltA"
                     /locus_tag="ACHJ7J_001629"
     CDS             complement(12122..13219)
                     /gene="mltA"
                     /locus_tag="ACHJ7J_001629"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO4999881.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13428..13504
                     /locus_tag="ACHJ7J_001630"
     tRNA            13428..13504
                     /locus_tag="ACHJ7J_001630"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13462..13464,aa:Met,seq:cat)
     gene            complement(13578..14831)
                     /gene="amiC"
                     /locus_tag="ACHJ7J_001631"
     CDS             complement(13578..14831)
                     /gene="amiC"
                     /locus_tag="ACHJ7J_001631"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO4999882.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15063..16394
                     /gene="argA"
                     /locus_tag="ACHJ7J_001632"
     CDS             15063..16394
                     /gene="argA"
                     /locus_tag="ACHJ7J_001632"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO4999883.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQSRVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16456..18282)
                     /gene="recD"
                     /locus_tag="ACHJ7J_001633"
     CDS             complement(16456..18282)
                     /gene="recD"
                     /locus_tag="ACHJ7J_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO4999884.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALIVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18282..21824)
                     /gene="recB"
                     /locus_tag="ACHJ7J_001634"
     CDS             complement(18282..21824)
                     /gene="recB"
                     /locus_tag="ACHJ7J_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO4999885.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYSLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMVAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(21817..24705)
                     /gene="ptrA"
                     /locus_tag="ACHJ7J_001635"
     CDS             complement(21817..24705)
                     /gene="ptrA"
                     /locus_tag="ACHJ7J_001635"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO4999886.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVQHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISSKEPHNKTAYFVDAPYQVDKISTQTFANWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24881..28249)
                     /gene="recC"
                     /locus_tag="ACHJ7J_001636"
     CDS             complement(24881..28249)
                     /gene="recC"
                     /locus_tag="ACHJ7J_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO4999887.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWWQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28262..28585)
                     /gene="ppdC"
                     /locus_tag="ACHJ7J_001637"
     CDS             complement(28262..28585)
                     /gene="ppdC"
                     /locus_tag="ACHJ7J_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO4999888.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(28570..28977)
                     /gene="ygdB"
                     /locus_tag="ACHJ7J_001638"
     CDS             complement(28570..28977)
                     /gene="ygdB"
                     /locus_tag="ACHJ7J_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO4999889.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLIAGYEGVSL
                     WRAGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(28974..29537)
                     /gene="ppdB"
                     /locus_tag="ACHJ7J_001639"
     CDS             complement(28974..29537)
                     /gene="ppdB"
                     /locus_tag="ACHJ7J_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4999890.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGAASCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(29528..29998)
                     /gene="ppdA"
                     /locus_tag="ACHJ7J_001640"
     CDS             complement(29528..29998)
                     /gene="ppdA"
                     /locus_tag="ACHJ7J_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4999891.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30183..30977)
                     /gene="thyA"
                     /locus_tag="ACHJ7J_001641"
     CDS             complement(30183..30977)
                     /gene="thyA"
                     /locus_tag="ACHJ7J_001641"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO4999892.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(30984..31859)
                     /gene="lgt"
                     /locus_tag="ACHJ7J_001642"
     CDS             complement(30984..31859)
                     /gene="lgt"
                     /locus_tag="ACHJ7J_001642"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO4999893.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32010..34256)
                     /gene="ptsP"
                     /locus_tag="ACHJ7J_001643"
     CDS             complement(32010..34256)
                     /gene="ptsP"
                     /locus_tag="ACHJ7J_001643"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4999894.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKLVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34269..34799)
                     /gene="rppH"
                     /locus_tag="ACHJ7J_001644"
     CDS             complement(34269..34799)
                     /gene="rppH"
                     /locus_tag="ACHJ7J_001644"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO4999895.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35152..35298)
                     /gene="ygdT"
                     /locus_tag="ACHJ7J_001645"
     CDS             complement(35152..35298)
                     /gene="ygdT"
                     /locus_tag="ACHJ7J_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO4999896.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFGGSVLNLVAHSVDK
                     YGIG"
     gene            35484..36173
                     /gene="mutH"
                     /locus_tag="ACHJ7J_001646"
     CDS             35484..36173
                     /gene="mutH"
                     /locus_tag="ACHJ7J_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO4999897.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36242..36955
                     /gene="ygdQ"
                     /locus_tag="ACHJ7J_001647"
     CDS             36242..36955
                     /gene="ygdQ"
                     /locus_tag="ACHJ7J_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4999898.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37093..37311
                     /gene="ygdR"
                     /locus_tag="ACHJ7J_001648"
     CDS             37093..37311
                     /gene="ygdR"
                     /locus_tag="ACHJ7J_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO4999899.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37419..38459
                     /gene="tas"
                     /locus_tag="ACHJ7J_001649"
     CDS             37419..38459
                     /gene="tas"
                     /locus_tag="ACHJ7J_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO4999900.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARHHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38492..39685)
                     /gene="lplT"
                     /locus_tag="ACHJ7J_001650"
     CDS             complement(38492..39685)
                     /gene="lplT"
                     /locus_tag="ACHJ7J_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO4999901.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39678..41837)
                     /gene="aas"
                     /locus_tag="ACHJ7J_001651"
     CDS             complement(39678..41837)
                     /gene="aas"
                     /locus_tag="ACHJ7J_001651"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO4999902.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            42423..43454
                     /gene="galR"
                     /locus_tag="ACHJ7J_001652"
     CDS             42423..43454
                     /gene="galR"
                     /locus_tag="ACHJ7J_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO4999903.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43461..44723)
                     /gene="lysA"
                     /locus_tag="ACHJ7J_001653"
     CDS             complement(43461..44723)
                     /gene="lysA"
                     /locus_tag="ACHJ7J_001653"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO4999904.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDRATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAARQQIASHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
BASE COUNT        10889 a        12128 c        11142 g        10579 t
ORIGIN      
        1 ttaatacggt catctggttt gtgtttttta tgatttattt tctgaaacgg gcatgaaatt
       61 tcgattatta aagtgatggt agtcacataa agtcaccttc cagctaataa gtgtgaccgc
      121 cgtcatatta cagagcgttt tttatttgaa aatgaatcca tgagttcatt tcagacaggc
      181 aaatattcac tgatatgaag cccgaactcg ctggttttgc acttttgaaa acataaccga
      241 ttacgtgctt aagcttctga acctaagagg atgctatggg aaacacatca atacaaacgc
      301 agagttaccg tgcggtagat aaagatgcag ggcaaagcag aagttacatt attccattcg
      361 cgctgctgtg ctcactgttt tttctttggg cggtagccaa taaccttaac gacattttat
      421 tacctcaatt ccagcaggct tttacgctga caaatttcca ggctggcctg atccaatcgg
      481 ccttttactt tggttatttc attatcccaa ttcctgctgg gatattgatg aaaaaactca
      541 gttataaagc aggaattatt accgggttat ttttatatgc cttgggtgct gcattattct
      601 ggcccgcggc agaaataatg aactacacct tatttttagt tggcctattt attattgcag
      661 ccggattagg ttgtctggaa actgccgcaa acccatttgt tacggtatta gggccggaaa
      721 gcagtggtca cttccgctta aatcttgcgc aaacatttaa ctcatttggc gcaattatcg
      781 cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc acatcaatcg caagacgttc
      841 tcgataaaat gtctccagag caattgagtg cgtataaaca cagcctggta ttatcggtac
      901 agacacctta tatgatcatc gtggctatcg tgttactggt cgccctgctg atcatgctga
      961 cgaaattccc ggcattgcag agtgataatc acagtgacgc caaacaagga tcgttctccg
     1021 catcgctttc tcgcctggcg cgtattcgcc actggcgctg ggcagtatta gcgcaattct
     1081 gctacgttgg cgcacaaacg gcctgctgga gctatttgat tcgctacgct gtagaagaaa
     1141 ttccaggtat gactgcaggc tttgccgcta actatttaac cggaaccatg gtgtgcttct
     1201 ttattggtcg tttcaccggt acctggctca tcagtcgctt cgcaccacac aaagtcctgg
     1261 ccgcctacgc attaatcgct atggcactgt gcctgatctc agccttcgct ggcggtcatg
     1321 tgggcttaat agccctgact ttatgcagcg cctttatgtc gattcagtac ccaacaatct
     1381 tctcgctggg cattaagaat ctcggccagg acaccaaata cggttcgtcc ttcatcgtta
     1441 tgaccatcat tggcggcggt attgtcactc cggtcatggg ttttgtcagt gacgcggcgg
     1501 gcaacatccc cactgctgaa ctgatccccg cactctgctt cgcggtcatc tttatctttg
     1561 cccgtttccg ttctcaaacg gcaactaact gaacatattt tccgaataaa gtgaggaatc
     1621 tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc ccggttattg acggtcgtcg
     1681 catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat atggcgaaag ctacggccgc
     1741 actgctgacc gagaaactgc gccatgcctg cggagctgcc gtcgagtgtg tcatttccga
     1801 tacctgtatc gcgggtatgg ctgaagccgc ggcttgcgaa gaaaaattca gcagtcagaa
     1861 tgtaggcctc accattacgg taacgccttg ttggtgctat ggcagtgaaa ccatcgacat
     1921 ggatccaacc cgcccgaagg ccatttgggg ctttaacggc actgaacgcc ccggcgctgt
     1981 ttacctggca gcggctctgg cagctcacag ccagaaaggc atcccggcat tctccattta
     2041 cggtcatgac gttcaggatg ccgatgacac atcgattcct gccgatgttg aagaaaaact
     2101 gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc
     2161 gctgggcggc gtttcgatgg gtatcgccgg ttccattgtt gatcacaact tctttgaatc
     2221 ctggctggga atgaaagtcc aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca
     2281 gaagatttac gacgaagccg aactggaaat ggcactgacc tgggctgata aaaacttccg
     2341 ctatggcgaa gatgaaaata acaaacagta tcaacgtaat gccgagcaaa gccgcgcagt
     2401 tctgcgcgaa agtttactga tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa
     2461 actggccgat attggtcgcg tggaagaatc acttggctac aacgccatcg ctgcgggctt
     2521 ccaggggcaa cgtcactgga ccgatcaata tcccaatggc gacaccgccg aagcgatcct
     2581 taacagttca tttgactgga atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga
     2641 cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc accggcaccg ctcaggtatt
     2701 tgccgatgtg cgtacctact ggtcaccaga agcaattgag cgtgtaacgg ggcataaact
     2761 cgatggactg gcagaacacg gcatcatcca tttgatcaac tcaggttctg ctgcgctgga
     2821 cggttcctgt aaacaacgcg acagcgaagg taacccgacg atgaagccgc actgggaaat
     2881 ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga
     2941 atacttccgt ggcggcggtt actcttcccg cttccttacc gaaggcggcg tcccgttcac
     3001 catgactcgt gtcaacatca tcaaaggcct gggaccggta ctgcaaatcg cggaaggctg
     3061 gagcgtggaa ttgccgaagg atgtgcatga catcctcaac aaacgcacca actcaacctg
     3121 gccaaccacc tggtttgcac cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc
     3181 ggtaatggcg aactggggcg ctaaccatgg ggttctgacc atcggccacg ttggcgcaga
     3241 ctttatcact ctcgcctcca tgctgcgtat cccggtatgt atgcacaacg ttgaagagac
     3301 caaagtgtat cgtccttctg cctgggctgc gcacggcatg gatattgaag gccaggatta
     3361 ccgcgcttgc cagaactacg gtccgttgta caagcgttaa tacattactc cctgatgtga
     3421 tgcccggtcg ctccggctac cgggcctgaa caagcaagag tggttagccg gataagcaat
     3481 gttatccggc tatattgcag gagcgattat gaaacaagaa gttatcctgg tactcgactg
     3541 tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc
     3601 ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac acctggcacc agtggtcttt
     3661 agacgccatt ttgcaacgct ttgctgattg ctgtcggcaa atcaatagtg aactgactga
     3721 atgccacatc cgcggtatcg ccgtcaccac ctttggtgtg gatggcgctc tggtagataa
     3781 gcaaggtaat ctgctctatc cgatcattag ctggaaatgt ccgcgaacag cagcggttat
     3841 ggacaatatt gaacggttaa tctccgcaca gcggttgcag gctatttctg gcgtcggagc
     3901 ctttagtttc aatacgttat ataagttggt gtggttgaaa gaaaatcatc cacaactgct
     3961 ggaacgcgcg cacgcctggc tctttatttc gtcgctgatt aaccaccgtt taaccggcga
     4021 attcactact gatatcacga tggccggaac cagccagatg ctggatatcc agcaacgcga
     4081 tttcagtccg caaattttac aagccaccgg tattccacgc cgactcttcc ctcgtctggt
     4141 ggaagcgggt gaacagattg gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc
     4201 cgttggcata ccggtgattt ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg
     4261 tgctgaacaa aatgaacccg tgctctcttc cggtacatgg gaaattttaa tggttcgcag
     4321 cgcccaggtt gatacttcgc tgttaagtca gtacgccggt tccacctgcg aactggatag
     4381 ccaggcaggg ttgtataacc caggtatgca atggctggca tccggcgtgc tggaatgggt
     4441 gagaaaactg ttctggacgg ctgaaacacc ctggcaaatg ttgattgaag aggctcgtct
     4501 gatcgcgcct ggcgcggatg gcgtaaaaat gcagtgtgat ttattgtcgt gtcagaacgc
     4561 tggctggcaa ggagtgacgc ttaataccac gcgggggcat ttctatcgcg cggcgctgga
     4621 agggttaact gcgcaattac agcgcaatct acagatgctg gaaaaaatcg gccactttaa
     4681 agcctctgaa ttattgttag tcggcggagg aagtcgcaac acattgtgga atcagattaa
     4741 agccaatatg cttgatattc cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg
     4801 agctgcgctg ttcggttggt atggcgtagg ggaatttaac agcccggaag aagcccgtgc
     4861 acagattcat tatcagtacc gttatttcta cccgcaaact gaacctgaat ttatagagga
     4921 agtgtgaaat gctgaaaaca atttcgccgt taatttctcc cgaactattg aaagtgctgg
     4981 cagagatggg acatggagat gaaattattt tttccgatgc tcactttccc gcccattcga
     5041 tgggaccgca ggtgatccgc gctgatggcc tgttggtgag cgacttgctc caggcgatta
     5101 tcccgttatt tgagctggac agttatgcac cgccgctggt gatgatggca gcggtagaag
     5161 gtgacactct cgatcctgaa gtagaacgac gttaccgtaa tgcgctttca ctacaagccc
     5221 cctgtcctga catcatccgc atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct
     5281 ttgcgatcgt tatcacaggc gaacgagcga agtacgggaa tattctttta aaaaaagggg
     5341 taacaccgta atctcatacc ggtacgcccg catgacgcgg gcggttatcg aatgatgggg
     5401 tgaaaaatat gaaagcggca cgccagcaag cgatagtcga cctgctgctg aaccatacca
     5461 gcctgaccac ggaagctctc tctgaacagc taaaggtcag taaagaaacc attcgtcgcg
     5521 atctcaatga attacagacg cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata
     5581 tccaccgtca aaatcaagac agtggcgatc cctttcacat ccggctgaaa agccattatg
     5641 cgcataaagc agatatcgcg cgcgaagcgc tcgcgtggat tgaagaaggg atggtgatag
     5701 ccttagacgc cagttcaacc tgctggtatc tggcacgcca gttgcctgac atcaacattc
     5761 aggtcttcac caatagccat ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac
     5821 tgatcagttc cggcggcaca cttgagcgca aatatggctg ttacgtcaat ccctcgctga
     5881 tttcccaact taaatcgctg gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata
     5941 gcagcggcgc actgtgggac tccaatgcta tcaacgctga ttacaaatcg atgctattaa
     6001 aacgtgccgc gcaatcgttg ttattgattg ataaaagtaa atttaatcgt tcaggggaag
     6061 cccgcatcgg gcatctggat gaggtaacgc acattatttc tgatgagcgc caggttgcaa
     6121 cttctttggt aacagcctga cggaaagggt agcaggccgg agacgacgtc ccggccttgc
     6181 ctgttatcgc tcgtcgcgac gaccacccac cgcagcccag atacggcgga cgtgcaccgt
     6241 cacttcttcg cgatcgtgat acaactgccg cgcctgaatc tgagcattta tgccatgttc
     6301 atcaagctgt gcctgaatat acgccagatt gtgtgatact tcttcgtagc gttttttcat
     6361 cggcagtttg aggttgaaaa tggtttcacg gcaccagcca ttaaccagcc actgcgccat
     6421 caatgccgca actttcgccg gtttttcaac catatcgcat accatccagg agatattgct
     6481 gcgtgtcgga cggaatttga acccgtcttc ccgcagccac gtcacctgtc cggtatccat
     6541 cagactttgc gccatcgggc cgttgtcgac ggaataaacc cacatgttgc gcttcaccag
     6601 ttggtaggtc cagccgccag ggcaagcgcc taaatccacc gcccacatcc cgttcgccag
     6661 gcgttcatcc cactcatccg caggaataaa cacatgaaat gcctcttcca gtttgagcgt
     6721 ggaacgactc ggcgcatctg ccggaaattt caggcgcgga atgcccatat agaacggcga
     6781 attattgttg ctgtatgagt aaccggtata gcagcagcct ggtgcaatga agaatacatg
     6841 cactaccgga cgcttcggcg tttcatagtt cgccagcacc cccgcatcgc gcaaggcagc
     6901 gcgtagcggc acggtgaatt tacggcagaa tttcagcaac tctttgcttt cgttggtatc
     6961 ggcaacttca acacgcagtt caccgccctt ctctactacg ccctgtaaca tgccgacaat
     7021 gggggtaata cgatcttctg gcggcaaatg ctgcaggagt tcccccacca caaaccactg
     7081 gcgggcaaaa attaatgaac tgaacggcaa ctcacggatt aacttatcgc catcatcagg
     7141 ttgataacat tcataaatga cataacccgc attctctttc acgcgggcaa aaccgaaaat
     7201 ttcccgctgg ccggctttat cggtaatttc tgcggcgcac tctttttcaa agcccggacg
     7261 gcacagcaat acaaccttat tcatgactta cgcccttgcg ctttaaacgg atagcaccaa
     7321 ctaacattaa cgcccagccc gcaaggaagc tcacgccgcc aaccggagtg acaaacgccc
     7381 acaaacgcag atgggacagt gccaggcaat aaaggctgcc gctgaacagc accgtgccta
     7441 acgcgaggaa aacgctactc cagtaaaacc agatactgat gcgacgctgc attgccaccg
     7501 ccagacctaa gatcgccagc gtatgaaacg cctggtattc gaggccggtc tggatccagc
     7561 ccatctcaac ggcccccatg gttttactta acacatgcgc accaaaagcg cccagtgcca
     7621 caaaaatgaa gccgctaatg gcggcgaaaa tcagcataaa acggctggtc atggtcgtac
     7681 cctacgtaaa ttattgttca taacgaaagc ggaatttttc ttgttcagca gcggctttcg
     7741 ccaggatcca ttggcgaaag gcggctattt tacccagttc tgcctggctg tcatgacaaa
     7801 ccagataaaa agcgttttta ctgaccagaa catcattaaa cgggcaaaca agacgtccgg
     7861 cttcaatttc agattgcgcc atcacgttat ttgccagcgc cactccctgc ccgtggatag
     7921 ccgcttgcag caccatggcg ctatggctaa aaattggccc ttgctgcacg ttgatatgat
     7981 ttaaccccaa ctgtcgggta tatgtctgcc agtcacggcg tgacgcatca tgtaataacg
     8041 tatgtttagc cagatcttcc ggggtcttca agggtttttc gccagtcagc agtagcggcg
     8101 aacacaccgg caataaatat tcggcgtaca gtttttccac ccgtagcccc ggccagttgc
     8161 cccgaccata aaatatcgcc acatcaacat catccgccag cttatcttcc tgacgatcaa
     8221 ccgcctggat tcgaacgtca attcccggat aagctgaatt aaagctggaa agtcgcggaa
     8281 ccaaccaatg aatggcgaaa ctagggagta aactgaccgt caacgccccc ttggcgctac
     8341 gggcctggag tttacgcgtc gcttcggtta attgcgaaaa tatctctttt atatcgagga
     8401 aatagctttg cccttcctcg gtcagcagga gtgaacgatt acggcggcgg aacagtttta
     8461 gccccaaaaa atcctcaaga gacttgattt gatgacttac tgcggcctgg gtcacaaaaa
     8521 gctcttctgc tgcgcgagtg aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg
     8581 catttagcgg tggtaatcgt ttagacatgg cttttaaact ttgatgttaa atgaatttaa
     8641 caattagatc acactgtgta acctattagt ttttttaatc tgagccatta taaattgtcc
     8701 gttgagcttc taccagcaaa tacctatagt ggcggcactt cctgagccgg aacgaaaagt
     8761 tttatcggaa tgcgtgttct ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt
     8821 gtttgcaatt ggtctgcgat tcagaccatg gtagcaaagc tacctttttt cacttcctgt
     8881 acatttaccc tgtctgtcca tagtgattaa tgtagcaccg cctaattgcg gtgctttttt
     8941 ttaccttgcg atcgcgaatt actgatccag ttcgaccatc tctttcacgt cggtacggtt
     9001 gatctgctgt ttgttgccat tagcgtcttt atacgaaatc ataccggtat cgttatcagt
     9061 ctgtggtttg ccgtcagaga cgatggtacg tccgtcattg gtgtgcatca catagttcga
     9121 accggaacag gcgctcaggg caaaagtcag catacaggca gaaataattg cggcagtctt
     9181 tttcatcatc ttctccttaa agcgattaat aaatcttaat agtattgatt aacaggctaa
     9241 aattaacgcc taacactatt cagcatatgt tactgacgcg gcttcgccag gatatccaga
     9301 taattctgat ggttagcact ctccttgtat caaagtgaat tttgcgtcac gatcggtgca
     9361 tcaagccgag gagttccatg aacgttttta atcccgcgca gtttcgcgcc cagtttcccg
     9421 cactacagga tgcgggcgtc tatctcgaca gcgccgcgac cgcgcttaaa cctgaagccg
     9481 tggttgaagc cactcgacag ttttacagcc tgagcgccgg aaacgtccat cgcagccagt
     9541 ttgccgaagc ccaacgcctg accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat
     9601 tactgaatgc accggatgat aaaactatcg tctggacgcg cggcaccact gaatccgtca
     9661 acatggtggc acaatgctat gcgcgtccgc gtctgcaacc gggcgatgag attattgtca
     9721 gcgtggcaga gcaccacgcc aacctcgttc cctggctgat ggtcgcccaa caaactggag
     9781 ccaaagtggt gaaattaccg cttaatgcgc agcggttgcc ggatgtcgat ttgttgccgg
     9841 aactgattac tccccgtagt cggattctgg cgttgggtca gatgtcgaac gttactggcg
     9901 gttgcccgga tctggcgcga gcgattacct ttgctcattc agccgggatg gtggtgatgg
     9961 ttgatggtgc tcagggggcg gtgcatttcc ccgcggatgt tcagcaactg gatatcgatt
    10021 tctatgcttt ctctggtcac aaactgtatg gcccgacggg tatcggcgtg ctgtatggca
    10081 aatcagaact gctggaagcg atgtcgccct ggctgggtgg cggcaaaatg attcacgaag
    10141 tgagttttga cggcttcacg actcaatctg cgccgtggaa actggaagca ggaacgccaa
    10201 atgtggctgg tgtcatagga ttaagcgcgg cgctggaatg gctggcagat tacgatatca
    10261 accaggccga aagctggagc cgtagtttag caacactggc ggaagatgcg ctggcgaaac
    10321 gtccaggctt ccgttcattc cgctgccagg attccagcct gctggccttt gattttgctg
    10381 gcgttcatca cagcgatatg gtgacgctgc tggcggagta cggtattgcc ttgcgggccg
    10441 ggcaacattg cgctcagccg ctactggcag aattaggcgt aaccggcaca ctgcgcgcct
    10501 cttttgcgcc atataataca aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg
    10561 cgctggaatt attggtggat taatgacgaa cccgcaattc gccggacatc cgttcggcac
    10621 aaccgtaacc gcagaaacgt tacgcaatac cttcgcaccg ttgacgcaat gggaagataa
    10681 atatcgccag ttgatcatgc tggggaaaca gcttccggct ttgccagacg agttaaaagc
    10741 gcaggctaaa gagattgccg gatgcgaaaa ccgcgtctgg ctgggatata ccgtggctga
    10801 aaacggtaaa atgcacttct ttggcgacag cgaaggacgt atagtgcgcg gcctgctggc
    10861 ggtgctgttg actgccgttg aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct
    10921 ggcattgttt gatgagctgg gattgcgtgc gcaacttagc gcctcacgca gccaggggtt
    10981 aaatgcgtta agcgaggcga ttatcgctgc gacgaagcag gtttaagcac agaaccgggt
    11041 cggataagac gttcgcgtct catccgacct gattgtttaa ccctgacgcg ccgctttcgc
    11101 tatcatcttc ttcagcgcat gagaaaccgc aacaaaacca aaggtggcgg tcaccatcgt
    11161 tgccgcgcca aatccagatg cacaatccat ccgctttggt ccttccgccg tggctttcat
    11221 cgcacacacc gtaccgtctg actgcgggta caccagcgct tcagtagaaa acacgcaatc
    11281 cacgccgagc ttacctttac tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg
    11341 caacttcgcc gccagcgggt cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt
    11401 cgggtcaatc tgcccacccg cgccaccggt cgtgaccagc gggattttat tgcgccgata
    11461 ataagcaatc agcgccgctt tgggccgtac actatcaatg gcatcaatca cgtacgaata
    11521 accggcactc atatattgcg ctacgttatc cggcgtcacg aaatcatcca ccaccgttac
    11581 acggcactcc gggttaatct ggcgaatacg ttccgccatg acttccgctt ttgccagccc
    11641 gacgttatcg cgcagagcgt gaatttgccg attggtattg gtgacgcaca catcatccat
    11701 atcgataagc gtgattgcgc caatccccgt gcgcgccagc gcttccgccg cccaggaacc
    11761 gacaccaccg ataccaacca cacaaatatg cgcgtcagca aacagttgca acgctttttc
    11821 accatacaga cgcgctgtgc caccaaaacg ctgacgccat gcatcactaa ttaccacaga
    11881 catagcacct cagaattaaa aagggcgagg ttatccctca ccctaaacgc ataaatgcct
    11941 gatgcgctac gcttatcagg cctacataac tcctgcaata ttttggattt gcatgatttg
    12001 taggcctgat aaggcgttca cgccgcatcc ggcatgaaca acgcgcactt agtcaataat
    12061 caggatgtga ggttacccct caccctgtca tatccgtaaa aacggcatac agaatatcac
    12121 atcagccact aaagacgtta cctgcgcccg gagccgtttt cagcacccaa acccgtccat
    12181 agtggttgta ccaacctgcg cggtgtccgg cttccggccc gatcccttga tagatatcga
    12241 agtgttggcc tttgattgcg ccaccgacat ccagcgccac catcagacgc aattcgtact
    12301 gaccgttaaa tttgccgtta ttatccagca gcggcacttc tgccagcaag gttgtacctg
    12361 gcggaataat ggaacgatca gaggcaactg aagcgcgacc aaccagcggc accgcacttg
    12421 cccctttcac cggtgcaaaa gattgcggtt taaagaagac aaaagacggg ttctgttcca
    12481 gcagctcgcg aacctcggct tcactgtgtg tttcgcccca gtgacgaatc gcctgcatcg
    12541 acatatcttc ttttttcact tcgccacggt cgatcagcac cttaccaatg ctgcgatagg
    12601 catgaccgtt tttccctgca tagctgaaaa agttaagcgg actgccatca ccaaagtcga
    12661 tatacccact cccctgcaca tccataatga agttatccat cagggagtta ctgtaagcga
    12721 gaatatattt atcactcaat gcccccgcgt agatctccgc acgagacggc agacgaccac
    12781 gttttggcgg catacggtaa ataggatact ggaactcgcc ctggcgggta tggcgcgcct
    12841 gaattaccgg tgtgtaataa ccggtaaact gcacgttacc atagttgtcg gcaccttcca
    12901 tctgccaggc atcaatgccg aactgacgca tattgcgggt atcaccgcct gcgcgcagcc
    12961 actcttgtac cgcgttataa acattactct ggttgccata cagacgcggt gacgaattac
    13021 ggatatggtt aatttgctcg gcaaaatcac cggcgttaat cggcgcgcca acggcatctg
    13081 gctggttcac cagagagaaa ggctgggtaa atttcccgtc tttatattgc tgtccgcgat
    13141 cggttggttt ggaagagcag gcggcaagca ttgccacaac cgtgcccata agaaggtact
    13201 ttacccaacg tcctttcatt gttctcttct tcaggttaaa aaataaggcg caacgaagat
    13261 aacaaaccac cgcgtttaat gaaatgagca gcctctccct gacgcaaatt ttgcacaaaa
    13321 aataggcttt agtgatttgt ttttgttcaa aatcatgcca gatccgtgat cggggtaaaa
    13381 aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat ccacggacgc ggggtggagc
    13441 agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc ggcccccgca
    13501 accaattatt gaacacccta acgggtgttt ttttgtttct ggtctcctat aaaaaagcgc
    13561 cattcagcgc ctttttatca tccccttctc gccagcgtcg ccccatcggc aaaatacgct
    13621 ttaatccctg caagaataga ctccgcaact tcctgctgga aggtcgccgt tttcagttta
    13681 cgctcttcct caacgttact tataaacgcc gtttcgacca gaatggaggg aatatctggt
    13741 gcctttagta cggcaaaccc ggcctgttca acttgatttt tatgcagctt gttgatttta
    13801 ccgagcttat tcagcaccgc tttaccaaac ttaaggctgt cagcaatggt cagcgactgc
    13861 accatatcga acatggtgtg gtcgacatag cggtcaccgc ttttgctcac gccaccaatc
    13921 aagtcggagg cgttctgggt ttgtgccaga tattttgccg cagtactggt tgcacctttg
    13981 gttgagagcg caaacaccga ggaaccgctc ggctggcgac tggtaaaggc gtcggcatgg
    14041 atagagacaa acaggtcagc acgctgtttc tgagcttttg ctacgcgcac ttgcaacgga
    14101 atgaagatgt cttcattgcg cgtcatgtac accttcatat tgccctcttt ctcgatcaga
    14161 gagcgcagac ggcgagctat ttgcaatacc acgtcttttt cgcgcgtttt gtatttcccc
    14221 accgcaccgg agtcttcgcc accgtggcca gggtcaagca taatgacaat cggacgatcg
    14281 cgccctgctt tacctggttg tggaccactt tgcgctggcg gcacctgctt ttcgaggtcg
    14341 cctttgttgt aatcctccag cagcgccagc agcgggtcct gcatatcctg cgcattggcc
    14401 ggatagaggt ccatcaccag acgctcttta aacccggcga ccggcgcaag ggcaaacagc
    14461 tgcggtttta cgttttgctt taattcaaaa accatgcgta cggtttgcgg gtcaaattgc
    14521 ccgacgcgcg ccgacttgat gaacgggtcg tctgcacgaa tttgtgccgc catccccttg
    14581 agtaccgagt tcaggtttac atcttcgata tccaccacca cgcgttccgg gttactcaac
    14641 gcgaactgct tatatttcag ctgacgattt gattctaccg tcacgcgggt gtagctggac
    14701 gcaggccaga cgcgcaccgc cacgacctgg ctgaccgcag ccaggctgac ctgacttacg
    14761 ctcaatagcc acatggcgcc cgcgccttgc agtaaacgac gacggctgat tgcagtgttg
    14821 gatcctgaca tgcctctccc gagcaaaaga aatctaagct gtgtaacaag taaacgacta
    14881 atttgaccgg aaactttagc gaaagacgca taatctgtca tctaataaac ggtaaacatt
    14941 ctttttatat tcacggcatt actgataaaa aagtcgctct cgcataaaat ttacacttgc
    15001 accctgcgaa aaaacagaat aaaaatacaa taatttcgaa taatcatgca aagaggtgtg
    15061 ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt ccgccattcg gttccctata
    15121 tcaataccca ccggggaaaa acgtttgtca tcatgctcgg cggtgaagcc attgagcatg
    15181 agaatttctc cagtatcgtt aatgatatcg ggttgttgca cagcctcggc atccgtctgg
    15241 tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct ggctgcgcat caccacgaac
    15301 cgctgtatca caagaatata cgtgtgaccg acgccaaaac actggaactg gtgaagcagg
    15361 ctgcgggaac attgcaactg gatattactg ctcgcctgtc gatgagtctc aataacacgc
    15421 cgctgcaggg cgcgcatatc aacgtcgtca gtggcaattt tattattgcc cagccgctgg
    15481 gcgtcgatga cggcgtggat tactgccata gcgggcgtat ccggcggatt gatgaagacg
    15541 cgatccatcg tcaactggac agcggtgcaa tagtgctaat ggggccggtc gctgtttcag
    15601 tcactggcga gagctttaat ctgacctcgg aagagattgc tactcaactg gccatcaaac
    15661 tgaaagctga aaagatgatt ggtttttgct cttcccaggg cgtcactaat gacgacggtg
    15721 atattgtctc cgaacttttc cctaacgaag cgcaatcgcg ggtagaagcc caggaagaga
    15781 aaggcgatta caactccggt acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca
    15841 gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga tggcgcgctg ttgcaagagt
    15901 tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc
    15961 gcgcaacaat caacgatatt ggcggtattc tggagttgat tcgcccactg gagcagcaag
    16021 gtattctggt acgccgttct cgcgagcagc tggagatgga aatcgataaa ttcactatta
    16081 ttcagcgcga taacacgact attgcctgcg ccgcgctcta tccgttcccg gaagagaaga
    16141 ttggggaaat ggcctgtgtg gctgttcacc cggattaccg cagctcatca cggggcgagg
    16201 ttctgctgga acgcattgcc gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc
    16261 tgaccacgcg cagtattcac tggttccagg aacgtggatt taccccagtg gatattgatt
    16321 tactgcccga gagcaaaaag cagttgtaca actaccagcg taaatccaaa gtgttgatgg
    16381 cggatttggg gtaagcgacg aaaatcgccg gatgcggcag gcgtaacact cgtacgtcgc
    16441 atccggcaat tacgtttatt cccgtgaact aaacaacgcc gccagaccac tgcgccgctc
    16501 agtacgagtg gcgattgccg cacttaatat gcgctcatcg gcatacagcg acagacggcg
    16561 acgcgcgcgg gtcaccgcgg tataaaccag ctctcgcgtt actaccggcg tgcgttggct
    16621 cggcaaaatc aacgccgcat ggtcgaactc cgaaccctgc gatttatgta ccgtcatcgc
    16681 ccacgtagtt tcgtgctctg gcaaacgact cggttgcaca gacttaatat tgccgtccgg
    16741 catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccga tatcgccatt
    16801 aaacaaccca agcgcgctgt cattacgggc aatcatcacc ggtcggcctt cgtaccaacg
    16861 agagtgcgga ttacgatgaa ttttgcgctt ctgttgcata aactgctcaa ttcgctcatt
    16921 cagtccagcc acgccaaatg gcccttcccg cagggcgcac aaaagttggt actcattgaa
    16981 cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat aacgtccata
    17041 acccgcaaga gcttcctcaa gcatcgcaat ataatcttcg ccgctctgta aaagccgttt
    17101 ttcgatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat caccacggtt
    17161 gatcgccgca gctaactgac caatgccaga atcgctgccg aaacgatagc ttttttgcag
    17221 caggcagaga ctgtcgcgca aagatgccgc ttctgtgcca gttcctgccg gaacgtgagt
    17281 tcccgtcagg cggcttagct gcctggcacg ctcggcggta aagcccgcgt tggcataagc
    17341 gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac gatcgccgag
    17401 aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt ctcgacatca taggcagatc
    17461 gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac cggcatgatg
    17521 acgtaaacgc tggctacccg gctgcgcgcc cagcaatcgg tgcaaagtgc tggcatcttc
    17581 cggaatgcgt ttcttttgtt catcggtcag cggtaactgt cgcaaagcct tgccgagaga
    17641 ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac ggcagcgttc
    17701 gccgtcggcc atttgaatta acgctgccag caacttcgcc acggtggtcg ttttaccggt
    17761 gccagggccg ccggaaatca ccgagatccg ccgcgtcagc gccactgccg ctgcaacttt
    17821 ttgccagtta atttcatcgc ttactgggaa aagtttgtcc agggtttgcg ccagtagagc
    17881 ttcatcaacc tcgatggcat gattcacctc attgaagaag cgcgctaccg tccgctcgtt
    17941 acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg ttggttcatc
    18001 tccccggctg accgcttgtg aagccaacaa gcattcttcc caattttgta gctcaccgat
    18061 ttcactgaca caggtcgcca acagcggatg cgactcctcg ttattttcca gtcgtgaaag
    18121 cggcaaacaa acgtgtccct ctccggcatc atgacttaat agtgccgccg cgagtgtgac
    18181 ggcaggatgt tcatctcccg ccacgatcag ggcaaactgc acgtccagcg ggcgtagctg
    18241 tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc ctccagggtc
    18301 ataccggcaa acatctcatc catcagggca atcaacccgg cgttgggtcg ggttgtgtaa
    18361 atcccctgtt gcggatgttc tttatcaacg ccacgcagga acagataaat aacgccgcca
    18421 aagtggcgct catagtcgta atcagcaatg cgatggcgca gataacgatg cagcgccagg
    18481 gtataaagct gatattgcag atcatagcgg tgtgcctgca ttgccgctac catagcctgt
    18541 tgggtgtaag ccgaactgtc ttcacccaac cagttggatt tatagtcgag caggtaataa
    18601 cgcccttcgt ggcggaacac caggtcgata aagcctttta acatgccacg tacctgcatg
    18661 aactccagcg gcgggcagcc tgcggatagc gggtcaaact ggcggattaa cgtatcaagc
    18721 tgactggcga taagcggttc actaatcggc agataaaact ccatctccac ctgtttattg
    18781 cgggcggaaa gttgactcag gcttacgccg gtttcattga gaggtgcctg gaggacagcc
    18841 gtgatccact cggtcaatac cggttcccac tgcgattcaa agccgccgag ttccagtttt
    18901 tcccgcaccc agttcgggtc aaccggctgg gtaaaatcca ggtcttcaaa caaactgtgc
    18961 aagaacgtcc ccggtgacgc accgcgcgga aactgatgtg gtgttaacgt cggttcttca
    19021 acgacgctgg caacgcctgc agcatcgaca tccagccgag gcatcaaatc ctgggcgata
    19081 ccgtgacccc gctgctgcaa gccggagtag ctggtgacac gccagttatc gccgggcaat
    19141 cgttgtaacg tcttcgcatt cagctctgct gtagaaacat cattaacctg ccagggttgg
    19201 ttatcaccag tttgtgccgt ttgccaggca atatcatcat cgcataacgc ttcaatacag
    19261 gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca gcaaacgccc gagcgcactt
    19321 tggtggacgt cggtgtcacc ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg
    19381 agactacaat gccaaaccga acgcgtcagc gccacgtaaa gcaaacgcag atcttccgcc
    19441 agacgttcgg cctccgcgag atcgacactt tctggcgcag cgttaagatc cagaacagcc
    19501 tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt ggtaataaat
    19561 ggcaaccaga caagcgaata ttccagccct tttgatttat ggatcgtgac aatctgcacc
    19621 agatgtttat cactttcgag gcgcatttgt tggctggagg cattactgtc tggctcgagg
    19681 atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt tccggcttct
    19741 tgtagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc tgccgttgcc
    19801 agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc gcagcatcgg cataacgcca
    19861 cgtttgcgcc agatttgccg ataaccatcg aactcttcga ccaccgcgtc ccacgcatgt
    19921 tcgtcattat tcagcgtttc gatatccagc gcgttcagcc ccatcattga cgttgccagc
    19981 gcactacgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacaa ccaaagcatt
    20041 tcttgcgctt ccagagtttc aaaaacactg tcgcggttcg aaaggtaaac ggaagggatt
    20101 tccagcaacg ttaaggcatc gcgcacctgg gcagcctcct ggcggctgcg caccagcaca
    20161 ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt tcatcagcaa cgcttcgccc
    20221 cgctgtccgg cttgtagcca gtcgcggatt tgcgcagcac atacctgcgc catggtactt
    20281 tgatagtcgc caacgccgca gctttcgcct tccatcagcc acattttcat cgcaggctgc
    20341 gtttcacctt taaatacaaa acgtaacgcc tgatttttcc cggctgattt cactggaata
    20401 aacggtattt cgcgaaacat gaacgcgtca tcagtctggc tgaaaagctt gttcacgctg
    20461 ttcaccattc ctggtgcgga acgccagttg gtgtctaaag tgtagtgggc gtgaacttcg
    20521 ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat ggcctgcttc
    20581 gggtcgccaa ttagcaacaa tgcggtttcc ggctgatggt gccagatacg gcgaaaaatt
    20641 cggtactgct gggggtcggt atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta
    20701 cggatcgccg ctgccaacac ctcaccgctt tcgctacgca gcgcggaatc gagccgactt
    20761 aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg
    20821 atctcagcca atgcgcgggt gatcaccaga tcgcggatcg acaatggttc tgcaagcagt
    20881 tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcctt cgtgcgatct
    20941 tctaagaaac gctgggagaa tttttccagc gactccggca actgataact gtttgtctct
    21001 tcttctgccc aggcgctgat cttgtcgatc catttagcct gattgctacg gttaaacttg
    21061 cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc gtcgcgccac
    21121 tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt gacgggaagc cagcgtttca
    21181 tcatcgggcg gcggtgcttt gataaccggc gcttcgcctt gcagataacg attaatatcg
    21241 cgcagcaacg cctgcggccc tttccaggtt tcaaagacaa cctgggctat ttcacgcggc
    21301 agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtagcgtag cagagactca
    21361 tcttcaatca gctgctgctc aaacagcatg ccggattcaa aggcattcag gttgagcatg
    21421 cgctggcaaa agccgtgaat agtaaagact gccgcttcat ccatctgccg ttcggctaac
    21481 aacaaccact gcgcggcttg cgctttatcg tcgatctctt ccagcaggcg ttcgtacagt
    21541 ggattgtcgg tggtttcacg cagacaggcg atgcgcaact cgtggatatt gctacggata
    21601 cgaccgcgca attctgccgt ggcagcctcg gtaaaggtga ccaccagcag ttcttcaacg
    21661 gtcagcgggc ggggaaaggc ggcggaaccg cctagtccaa gtaacaggcg caaatagagc
    21721 gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg ctcgccctgt
    21781 aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt cactcatcag
    21841 gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt caggatgcac
    21901 atattcggct ttcccgttct ggctgccgga aatctgcgac agaatagcca tgccttgcgg
    21961 ctcgaccacc gcctgatgga agaaatcagc aagtttttgc ggcgtcagca gttttatctg
    22021 ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat ctttacttaa
    22081 cttcgatgct tcttcgccga gcgtttgcgg tgcctgcagc atctgggtaa ttaccgcctg
    22141 ctggatttgc gcaaactctt ctggcttcat cgcccgcaat tttgcctctg cagttgggaa
    22201 aaacgccttg taacgctccc acaagaatga aggctgttta tcattgcttt gcaaaaggaa
    22261 gcccatgccc cactgacgcc ccacgctcat tggaaacgca aacacggcat agcccaactg
    22321 ttcttcggta cgcaactgat tgtagaacca cggctgtacg atctgcccca acagagagct
    22381 ataggctgag ctggtgtatt catcgtagcc agtcggtaca aataccgctg ccagtgcgga
    22441 gtcggtgctg ttaccggctt tttcaaagat gacggattgt tttttatcga ccactacatc
    22501 tttgtttcga caccactctg acccatcagc gcccaactgt ttttgcacat cgcgtgccag
    22561 cgttgttgcc tgggcctcgg tcatgttgcc gataaccata aactctggtc gagcccctga
    22621 ttttaaggcg tcgcgatagg ccagcacctc tttcaacgta atggagggca aaattttgcg
    22681 ccgttcatct cgcgagaagt acggcacttg cgagagcatc tgcgcgggca taatcgcctg
    22741 ctcaaacgct ttaccctttt ctgcggaatc catcatctgg ttataccagg acttcgcctg
    22801 ctcaagctga tcttccgtag cggtatagct aaagtacccc tcgagcaatg cctggaacag
    22861 ctgcggcaga cgctgggtgt aaccattagc attaaccata aggccgttgt tagcgttggt
    22921 ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac tgatcaagcg ccagccctgc
    22981 gagataatca ttgagcgcaa acatcacctg attgcgggcg ctgtccatgg ctttcggatt
    23041 acgcaaaatc aggctgacat cagctttggg ttcgctggaa aaataacggc ttggcgcata
    23101 caccacgcgc agattcgact catcaacaat cagctctgga tggtcatatt tcttctctga
    23161 cttaatcagc gagaaatcgt caggaatata ggggttaagc tccggtaagg agagcgcaat
    23221 attggcagct ttttgctgcc agttagcgaa agtttgtgtg ctaattttat cgacctgata
    23281 cggcgcatcg acaaaataag ccgttttatt gtgcggctct ttcgagctga tataccagat
    23341 acgcgcattc tgcggcgtca tcatcgccag acgttccttt actgctttag catcgtaccg
    23401 atcggcaata ttgactgcat ccagcgtatg ctgaacagga acgcgaatca tggtatctgc
    23461 cagccattcg acgtaatcca tatcacgggt gatcgacgga taacggaagt cgatatccag
    23521 cacattcgcc agttcatcga agtattgttt atcaatgcct ttttcacgta acagattgag
    23581 atagctaaaa attgccgcca caacctgatc gcgattagcc aggcctttat cggttaaaga
    23641 cgcagagatc gctaatacgc cgctgttgcc gttgacgata ggatcggagt tggcgctaat
    23701 gccctcaact aatccctgct tttgcagcca gtcagaaagt gtacctgggc tgcgattgcc
    23761 aatcagatag gtaatcaatt catcggtttt actacggaac ttcgctgagt tgttatcgat
    23821 gcgaaactca acgcgcaaca ctttacgcgg cagcgcaggg acgtaatgaa tgataatgcc
    23881 cttttgcgcg tcggtgacta ccggcacggt gatttccggt tttttgctct ctttgttcgg
    23941 cacgcgacca aaggtgtccg ccgccatttt tgccaactcc ggcagcggtt tattactgta
    24001 aataaccgcc ttcatcaaat tggcggaata gtacttctcg tggaaatctt tcagcgcctg
    24061 ctgcaccgga ttaccaggtt tgtcgcttaa agtttcgagg ttaccaccag aaaactttga
    24121 accggggtgt gccgggttaa tggtttctgc gctgacctgt gccatgcgca tcccgtcacg
    24181 cgtacgcgcc atggttaatt cagcgttcac tgcattacgc tcacgttcgg cgtatttctt
    24241 gtcgagcaag ggttctgcaa tagcatcggc caggcggtct accgcaccgg gcaaggcgtc
    24301 gttctcaact tccagataga aagccgtgcg atacggcgca gtgctggcat tgtgactacc
    24361 gccgtgcatt ttgagatatt cggccagact gtcagcctgc gggtactttt tcgaccccat
    24421 cagactcata tgttcaaggt aatgtgccag cccctggtac gcctcgggat cttccagcga
    24481 cccaacgggc accaccagcg ccgatagcga tttaactgcc tgcggatcag aaaccagcaa
    24541 gaccaccata ccgttatcca gacgtatagc ctgatactgg cggttatctt tatcactttt
    24601 acggatggtt tcctgaatcg gctgccatcc cgtttctgcc tgacttaagg gtgcccaaag
    24661 ggcaactaac aacaataatg ctttgaacca ggtgctgcgg ggcattcacg gacctcataa
    24721 tcaactttat tttctggcca gattcaacag gttaacgcaa atcatctgcc agaatttaat
    24781 cttgtgctgc acgagtcagc ctatgtttat ataaccatca gtccgtgact ggtgcgcatc
    24841 ataaagtaag cggatagatt gcgcaatttt tatacagcac tcatgactga ttaaagcgaa
    24901 acagcggtaa caggaaacgt tgcgactgtt caacgattgc ctccattgtc tctggtgtta
    24961 attgccacca gagcctttga taccagatat catcaccttc gccacgcacc atcatgttgc
    25021 cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa cgtggaatcg tcatccagca
    25081 tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc gccgccactt tcaggtaaca
    25141 ccagcaatgg cgcggacatc ccttcacgat acccctcaat cagttgtgag aggtaatgca
    25201 aagcctgttc ggctgcaagc ggcggaaaac gccactcgcc gtctttgcgt agaaaaaggc
    25261 gactttcacc attaccaccg ctggcacagt agacaaggtg ttccagccaa agttgcattc
    25321 cctgcgccac acttaataaa gagggacgcc agcgcaacaa gccatccggc tgcacctgcg
    25381 gcaaccaacc agttatctgc acaccgttgc aagcgagatc aatttccata ctctgccccg
    25441 gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat ctcctggcac tgtgtttccc
    25501 agaaaatttc accaaaagcg ccatacggta aatcccctgc cgcccggaag cggcggaaca
    25561 agcgttcggc atcatcctgc tcaaccagtg cattcaataa ctgctgattg atttgataac
    25621 ggctaagtcc ttccagaata aatggctcgg tgtcggggat ttcgctgtct tcagtacgga
    25681 agttcacctg caaacgcatc tggaagaacg cccgcaccgg atgtgcccag aatcgttgta
    25741 gcgtttccag cggcacggtt tccggtaagg taaacggcag cggctgaaca aattcagaat
    25801 gtgctttacc agcctggctg gccgcaggta gccattcacg agcatagctt tgtcgttcgc
    25861 ctggctggta gttttgtgga tcaaacggca tccgggtatg gaggcaagta agatgcgctt
    25921 ttacccttgc ctcgctttca tcacagttga gcgcttcatc gcccggtaga taatgacttt
    25981 gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg gaaacgttca ctgttatcct
    26041 gaatggagcg cccgatatag ctgatataga gtttttgctg cgcggaaatt aacgcttcca
    26101 ggaacaggta gcggtcgtca tcgcgacggc tacggtcgcc acgcttcggt ttctggctca
    26161 tcaggtcaaa gcccaatggc gcaagctgac gtggataaac gccgtcgttc attcccagca
    26221 ggcaaaccac tttgaacgga attgaacgca ttggcatcag agtacaaatg ttaaccggcc
    26281 cggcgagaaa acgctggctg atacgttctt gatccaggcg ctgtgccagt tcatcacgca
    26341 atagtgacag cggcaccgca tcgccatact gcgcacctaa accttcggcg ataatcgcct
    26401 gccattgttg ttcgatcagc gtcatcgccg cttcggtttc cgcatccggc aggaagaagg
    26461 cgttgagcat atcgcgacaa accggcaacc actcttccag cggacgctcc tgtgcaagcc
    26521 cgcgacgcca gatgtttagc tgcattagca gtgaagccag atgccccacc agttctgcaa
    26581 ttaagccgct cgattcatca taaggtagaa ccgattgcca ctcgccctgc gcgctctcca
    26641 tcgcgtagcc caacaacata cgcgtcaggc caaatcgcca ggtgtgttgt ccggtggcgg
    26701 ggagttccag ctcgcgaacg ttgtcgtcat ctatgcccca acgaatgccg gactcgttga
    26761 cccactggcg taaataacgc agcccttctt cggtgatgtc aaaccgcgcc gccagcaccg
    26821 gcacatccag cagcgccagc acatcctctg acacaaagcg actgtcaggc agtgataaca
    26881 ggctgataaa cgcttccagt accggatgtg actgccgcgc acgacggtcg gaaatggcgt
    26941 aaggcaggta acgatccgca ggtgcgctac caaacactgc ctgaataaac ggactgtagc
    27001 tgtcgatgtc ggccaccatc acgatgatgt cgcgcggagt aagtgtcggg tcttcctcca
    27061 gcatcgccag caggcgatcg tgcaaaactt caacttcacg ctgcgggcta tggcaaacgt
    27121 ggaaggtgat actgctatcc agtggatcaa gcgggcgttt gttatcgcta cgggaaaact
    27181 cttcgatgtt cacaccagca acagcgcggt tttccagttc cagaatgtca gactgaatgt
    27241 tatgcagcag gttatctggc gtgacatcga caaaggcgtc cagctcctgg ctgctctcca
    27301 ggtcagaaag gagataaatg tagtcgcgcc caagcttgcc ccatgaggcc agcagcgggt
    27361 tgccgacatc ctgttcacca tcgctgttaa agagctgccc ggcattttcg ctgtcgcgaa
    27421 atagcggtaa ttcgcgatct tcaaaactgt gtcggcgctg gcgagtcagt agtttcgcca
    27481 gataagcagg atctttaatg tcgccccagt aataacggca ggggttggta aacaggagat
    27541 ggatttcaat atgtttaccc agcgcctgga gcgcctggag ataaacaggc ggtaacgcgg
    27601 aaataccgca tataaagacg cgcgaaggta accccggcgg gcaggtcgtc gcggactcca
    27661 gcgtttcgat aaagcgctga tagagattgg cgcggtgcca acgcggttgt ccgagttcat
    27721 gtgtatattc caccagcgcc ttccacaacg gcgcttgcca ggcctgtgcc tctcccagcc
    27781 cttcaaccaa atgtcctgtt tcccactgtg ccagccaatc cggacgatag accagatact
    27841 ggtcaaacag gtccgccgct tttgaggaaa gctggaacag ttttcgcttg tcgctatcgt
    27901 cagtcagata atgccgcaac agggtaaagt cttcgcgatc cagcaattgc ggcagcagag
    27961 tcatcagttt ccagctcatg ctctgtttgt taaaggcgct ctctttgggg atctccggta
    28021 acacccggac gaacatatcc cagataaagc tcgcaggcag cggaaaatca atgtttgccg
    28081 caataccaaa cttttgcgac agggtcattt gcagccactg tgccataccg gtactttgca
    28141 ccagaatcat ctctggttcg aaaggatcgt ccagccgttc gcgttcgaca ataaactcca
    28201 tcaacgcttc cagcacgtcc agacgattgg aatggtagac ccttaacata gcggctcctg
    28261 actactgacg attcgggcaa tgcaggcggg tcatctcgcc tgctctgccc ccgggtgaaa
    28321 ctagcgtaac gctgatgctg acacatcccg cctgcgatgt ctgcattcgg ttgacctgcc
    28381 agttggcagg tggcgaaatc gcgcgcagtt gcgtttgctg ccaaccatgc cgccagagct
    28441 gttggtactg gtttctgctg gcaagactgt tcattaatgt tcgctggaaa cccgataacg
    28501 cagtgacaat catcaccatc aacaccatcg ccaacattac ctccggcagg ctaaagcctt
    28561 gttgattctt cagggaagct gacataacgc cccctctttc agcggacaaa aatcgctcca
    28621 gccgcgtggc gaaaaaacaa tttttccatc gatgacttca cctgctcgcc acaacgaaac
    28681 gccttcataa ccggcaatca ataaggcttc attatctgcc aataaacgca aacaaacccg
    28741 ggcatcggtt ccagcgtact gcgagcactg aactgctggc tgcgtctgcc aggagtgcat
    28801 ttttccccac gccagcgccg actgaacgat ggcctggcgg cgcaatgact gactttccat
    28861 gctcacgcga gaggcaaaac tgcgatcctg ttggctcatt ccttgtaata gcaagctacc
    28921 caaaactagc agcatcagga ccagagccag tgacgaaaca cccttttcgc ggttcacagg
    28981 ttgaatcctg tcacgctata gctggcattc accacggttt gcggttcaga cttactggca
    29041 gcacgcatat taaccgtcaa caccggcgag aagccgctga catcctgacg tacgacctga
    29101 aaagtgtcga taatgatggc atccggatta gtgactttat cccagccctt accttcacag
    29161 gatgccgcac cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc aatctggtcg
    29221 gactctttta ccggttcgcg atcccagata ccgttactgt tcgcatccca ctgcacaatg
    29281 acacagtcac cctgtccgac aatttccagc ccttcgccgg tacagatgcc atgacaataa
    29341 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagt
    29401 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
    29461 cccaacaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
    29521 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
    29581 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
    29641 cggtattgcg caggccaaag aaggcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
    29701 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg
    29761 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
    29821 gccagttggc atcttcacgt aaatagagca aatagtctcg cgcctggctg gcggtttgcc
    29881 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgca cttagcatga
    29941 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
    30001 cggcagtatg gagcaaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
    30061 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggtg tctcgaagaa
    30121 tttaatggag ggtaaaaaaa accgacgcac actggcgtcg gctctggcag gatgtttcgt
    30181 aattagatag ccaccggcgc tttaatgccc ggatgtggat cgtagccttc aatctcaaaa
    30241 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
    30301 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
    30361 gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cgcactgctg cgccatcata
    30421 tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaagac gtcacaggag
    30481 cgctgataaa gctgacaaga gagtttgccg tctgccacat agaactggaa gaatgcatgg
    30541 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
    30601 cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
    30661 ccatctggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccgttt
    30721 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
    30781 tcgccctgca gaaaccacag cagttcatgg atgatggaac gcaggtggca acgtttagtt
    30841 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
    30901 gttccggttc cggtacggtc gtttttctga gtgccttcgt cgagcacttt ttgcatcagt
    30961 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gcgggctgcg acgatatgcc
    31021 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg ccccatgctg
    31081 atgtactgca cccaggcacc ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
    31141 atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga cagctcccat tgggcgcggt
    31201 ttacgaatat acaggttgag gataataaac agcaccacac cttccagcag cagctcgtaa
    31261 agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga aaatggattg ccactgcggg
    31321 ttggtttgca gcagcaaaat atcttctgta cgggagcctg ggaacagcat ggcaaacggg
    31381 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
    31441 ccaagaccaa acggaatgag tggtgcgata aaatcagaaa cctggaagaa ggaacgttta
    31501 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaggac
    31561 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaaattg
    31621 tagaacagaa cataaccaat acgtcccccg aggaagacgc cgaggaagcc cgcatagagt
    31681 aagttttcaa cttcattttt ggtccagccg ctgcccggac gattcgcccg acgtgttgcc
    31741 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
    31801 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
    31861 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
    31921 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
    31981 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
    32041 ccgcgacgct ccataaaggc tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc
    32101 agactacgct gcgcaagatt ttctgcttcg gcataatcaa tgcgccgcag caggtatttt
    32161 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
    32221 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat tccatgtatt
    32281 tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag actgtcataa
    32341 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
    32401 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
    32461 ggtacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
    32521 acttcacgtc cggcacgttc aatcaggcgg cgagcttcgt caacttcatc gaggctggtg
    32581 accatcggca acagaatatt caggttgccc gtagcggcgt tagcacgcag catcgcccgc
    32641 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
    32701 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
    32761 gtacgcaagg tgacgagttt atcattgaac atttgcagca tcccctgata ctgcgccacc
    32821 tgttcttctt ccgacggaaa accactttgc agcatgaatg ggatttcagt gcgataaaga
    32881 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accggcattg
    32941 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
    33001 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtacc
    33061 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
    33121 agcaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc acgtaccatg
    33181 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
    33241 ggcaactcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
    33301 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
    33361 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
    33421 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
    33481 aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc
    33541 gccgtttctt tttgtgcacc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
    33601 gcttcttcca gcgccccggt cagtcgttcg cgttccagag ccggatccag cgttgatgcc
    33661 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
    33721 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
    33781 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
    33841 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
    33901 cgacgttgaa taattggtac gcctaaaaac gcgcggaagc gttcttcttt tacggagggg
    33961 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
    34021 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
    34081 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
    34141 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
    34201 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
    34261 ggcgtgactt aacctctttt acgtcgataa gcagatgcgt tttgtggttt tggcgtattt
    34321 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
    34381 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
    34441 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
    34501 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
    34561 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
    34621 aatcctactt cctcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggtta
    34681 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
    34741 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
    34801 ggactacctc aatataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
    34861 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
    34921 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
    34981 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
    35041 tagacatttt aaaatattca ttatcagtca atgcattaca tgtaatttaa gtaatgaaaa
    35101 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
    35161 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccaccaa aaactggagt
    35221 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
    35281 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
    35341 cccgttgcag tgttgcgcaa ctcgattacc ggcaacctaa aaagcaatat aaatcagaga
    35401 attgaacaac gcatgtggaa aaagttacac tgcgaatatt cggcacataa ttgctgtttg
    35461 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa
    35521 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
    35581 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataaaggc tggattggcg
    35641 tgttactgga gatctggcta ggtgccagcg caggaagtaa acctgagcaa gattttgctg
    35701 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
    35761 cattcgtttg tgttgccccg ttaacgggca atagcggggt gacctgggaa accagccacg
    35821 tgcgccacaa gctcaaacgc gtgctgtgga taccggttga aggcgagcgc agcatcccgc
    35881 tggcgcagcg tcgcgttgga tcaccgttgc tgtggagccc gaatgaagag gaagaccggc
    35941 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
    36001 ttaccgctcg tcacggggaa tatttacaga tacgaccgaa agcagcgaat gcgaaagcgc
    36061 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
    36121 tgaagaaaaa tttcaccagt gcgctgctgg cccgtcattt tctgatccag tagccatcgc
    36181 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
    36241 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
    36301 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
    36361 tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
    36421 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
    36481 attcagtcag gaaatttccg cccgtgactt gattctgctt ctgggtggct tgttccttat
    36541 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
    36601 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatcttcag
    36661 cctcgactcg gtgattaccg ctgtgggtct gtcagatcac ctgtttatta tgatggccgc
    36721 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
    36781 gcgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct
    36841 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
    36901 ctctattgcg gttgaaagcc tcaacctgat tcgcaacaaa aagaacccgc tctgatactc
    36961 cgtacgctct cctgcgggag ggcgtactca ctcctgttac cctcacagtt taagattttc
    37021 ctgctttcaa gatatataac gtcggtttat aaacagacta ttatcatagg tgggccaaat
    37081 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
    37141 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
    37201 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
    37261 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
    37321 tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt
    37381 tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc
    37441 acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca
    37501 gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg
    37561 acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct
    37621 acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag
    37681 tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga
    37741 agaatatccg cgaagcgttg catgacagcc tcaagcgtct gcagactgat tacctcgatc
    37801 tttatcaggt gcactggccg cagcgcccaa ccaactgctt cggcaaactc ggttatagct
    37861 ggacagattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc
    37921 aacgagcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc
    37981 gctacctgca tctggcggac aaacacgatc tgccgcgtat tgtcaccatt cagaaccctt
    38041 acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg
    38101 tcgaactgct ggcctattcg tgcctgggtt tcggcacgct gaccgggaaa tatctcaacg
    38161 gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg
    38221 agcaaacgca aaaagccgtc gcggcgtatg ttgatattgc cagacatcat ggactggatc
    38281 ctgctcagat ggcgctcgct tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg
    38341 gcgcaaccac gatggagcag ctgaaaacta acgtcgaaag tttacatctg gagttaagcg
    38401 aagacgtatt agctgaaatt gaagcggtac atcaggttta tacttatccg gcaccataaa
    38461 gattaacgcc ggttaaaacc ggcgttaaat attaatgacg gcgctgccag atccacagcg
    38521 ccgttattgc cagcgcaaat agcgcaccga agccaatgcc aatgggcacg accgggatgc
    38581 ctaccattac cgccagcgag taaatgccca gcatcaacaa catggcgctg ttttcgccaa
    38641 ggttttgtac tgcaatcgca ttccccgccc cgacgctttt tttaccccgc tcctgtagca
    38701 acgcattgag cggaacgaca aaaaagcccc ccagcacgcc aatcagcatc aacaaggcat
    38761 aggctggcaa cagctcgtgt tgcagggaga aaatcagtac caccacgcca atcaaaatcc
    38821 cggctggcat acagcgtgac acggtttcca gcgtaaccag cttcgctgcc gcacctgcgc
    38881 caaccacgat accaatcgct accatcgcgt tgagataggt cggcgtagcg ttatcggtaa
    38941 tgcccagcgc caccggtacc cacagcacca acaggaaacg cagcgttaca cccgctcccc
    39001 agaataaact ggtgcccacc agcgaaaaac gcgtttcacc attgcgccat agcgaggtgc
    39061 aggcattcag aaaactgcgg gtcatgttga tgagattcca ggactgcccc ggacgcgccg
    39121 ccgccagttt gggaatgtag atattggcaa cgaccgcacc accgtaggcc agtgcgcatg
    39181 cggccagggc aaccaggaca tgccagtcag ccagcacacc accggctacg gaaccgagca
    39241 aaatcgccgc tatggtagaa gcttccatta aaccgttagc ttttactaac ttactacccg
    39301 tggttaattc accgagaata ccgtatttcg ccggtgaata ggctgcagca ccaacaccca
    39361 ccagcgtata gccgagaaac ggattgatac caaagcagat actggctgcg cccagcagct
    39421 tcaggccgtt ggcaaacatc atcacccggc ctttggcgaa gctatccgcc acctgcccga
    39481 caaacggcgc aaaaagaatg taagcaccta caaacaccat ttgcaggatg ggctggctcc
    39541 actccggata gaactgcgct ttcagtaacg ccagagtggc aaacagtagt gcattatcgc
    39601 caaacgcaga gagaaactgc gccacgataa ccgctttcat ccccttcgac cacaacgaag
    39661 tgttagtgtg cactgactca ctcatcgtgt tgttccgctt cgtctaccca gcttttcaac
    39721 gtgacaaagt caggtttacc gctgccaagt aatggcatct gtttcagata gcgaatatcg
    39781 cgcggtacag caagctccgg cacgccgtgc tcgcgggcat actgttgcaa cttatcgcgc
    39841 gtcagttcgt tatctgtggt gaaaagcacc agtgcctcgc ctttgctggc atcgctctta
    39901 atcgcagtgg catggacttt atctggcgaa acaccaagtg ccagttgttc caccatttcc
    39961 aacgacacca tttcgcctgc aattttggca aagcgttttg cgcggccctg aatctgcaca
    40021 aagccctgct cgtcaaaacg cacgatatcg ccagtgtcat accagccgcg ctccatttcg
    40081 ccgcgaacat tctcggcggt gggcacttcc agtacaccag gcttctccac ccgcagatag
    40141 ccgttcatta tgttcggccc tttcagttgc aggcgtccgc cctcttcgat accaggaacc
    40201 gacaacaggc gcgcatccat tcctggtaga atacgcccta ccgtaccggg tttcgccgcc
    40261 atcggtacgt tgatagaaac gacaggcgcg cattcggtca caccgtagcc ttcaaggatg
    40321 cgcaggccaa atttatcctg ccaaagctgt ttggtacttt cttgtagttt ttctgcgcct
    40381 gccaccacat agcgtagacg atagaagtca tacgggttgg cgaaacgcgc gtagtgaccg
    40441 aggaaagtcg aggtgccgaa caacacggtg caactgcggt catacaccaa ctccggcaca
    40501 atgcggtaat gcagcgggct tggataaagg aacacttctg cacctgtgag cagtggcgta
    40561 aacaggccta ccgtcagccc aaaggagtga aacagcggca acgccgacat aaagcgatcg
    40621 ttggtggtga agtcggcaat cgttttaatc tgctcgacat tcgccagaat gcttttatgg
    40681 ctatggacga cgcctttcgg atggccttca gaaccggagg taaaaaggat cagcgcctct
    40741 tcttccggct gctgtttaac ctgtgccaga cgcggcatca gcaaatgagc gaagatccat
    40801 actttgtcgg cagtggtgac atctgctttt aaatcttcca gatagaccca gcgcacctga
    40861 gtaagttgct ccggcagatg ccagagtttg cctttatcga gaaactggcg ggaagtgaag
    40921 atggttttga tttcagccgc cgtaatggcg ctggtcagcc cttttacccc agcggtgtag
    40981 ttcatcattg cgggaatgcg gcgacgggcg atggccccaa aaatcactgc cgcactgatg
    41041 cctgcattgg gcagcattaa gccgatgcgt tcgccttcaa cactgtattt ttcaaggatg
    41101 cgtccaacaa acagcgtttt agtaagcaat ttgcgatagg agtctggggt aaagttgacg
    41161 tcttcgacac atttcttccc ggctccgaag cggtacattg cactcagtaa agattcgtac
    41221 agcgtttcac gcgggcgcac cgccatacgc gcttccatca ttatttgatg cagcatttcg
    41281 ccagcgattt tgcgacggtc acgggcacgc ggcgcatccg gcatctccac ctgcgttggt
    41341 ggcaaaatat gcagagtaat ttgcgggaac aagcgacgtt taaccagacc tttcaggcgg
    41401 ctgaagtggg taagttccgc cccttcaata cgcacaggaa taaccgttgc accagacttc
    41461 gccgcgacaa aacccgcgcc atcgtagatt ttcatcagcg agccagtggt ggtgattcgt
    41521 ccttcaggga aaatcaccac tggtcggccc tgttccacca gacgtaccag atgtttaatc
    41581 gccataggtt gcgtcgggtc gagaggaaca aagtcgataa atgatttcag ccaacgcata
    41641 taccactgtt ggcttattga ggtgtaaacg gcaaacactg gacgcacagg taaaaacagt
    41701 ccaagcaaaa tgccatcaat aaaagagacg tgattaggcg tgattagaac gcgctcgccc
    41761 ttcagtgcct gggtgtcacc cgtaacgcga acgcgataca aaacacggca caaatttcga
    41821 aaaaagctaa aaagcattcc aactcccttt gctctgattc agtaaaagcg aatggaggga
    41881 gattacacga gataaagaac gcgagcgaca gtaaattagg tgcgaaaaaa aacctgcgca
    41941 tccgcgcagg ttggtgcaag agacagggta cgaagagcgt accgaataat ctcaccaatc
    42001 aatacctctg ggatcttgat tgtggtctgc acgacgactc ttcgccagcg agaaaacgca
    42061 aaggaatgaa gggaaatgca acgaggtgtg taaattgtcg gttactgtta cagattgatg
    42121 accggcaaaa aaaaacctgc gcatctgcgc aggctggtgt aattcatgtg ctcaacccga
    42181 agttgacttc acctatcaat acctctggga tcaccacttt agcaacctga agccaaacgc
    42241 caccagcggt caatcgcaac agcgtttcgc aaagtgtaac caaaggtttg caatctcttt
    42301 ttttgtctta cttatttgcg tttggctcac attcccacga tgaaaacacg ccgccccttg
    42361 aaccaacggg cgttttccgt aacactgaaa gaatgtaagc gtttacccac taaggtattt
    42421 tcatggcgac cataaaggat gtagcccgac tggcaggcgt ttcagtcgcc accgtttccc
    42481 gcgtcattaa taattcaccc aaagccagcg aagcgtcccg gctggctgtg catagtgcaa
    42541 tggagtctct tagctatcac ccgaacgcca acgcccgtgc gctggcgcag cagaccactg
    42601 aaacggtcgg tctggtcgtt ggtgatgttt ccgatccgtt tttcggtgca atggtgaaag
    42661 cggtcgaaca ggtggcttat cacaccggta atttcttatt gattggcaac ggttaccaca
    42721 acgaacaaaa agagcgtcag gctattgagc aactgatccg ccatcgctgt gctgcgttgg
    42781 tcgtccatgc caaaatgatc ccggatgccg atttagcctc attaatgaaa caaatgcccg
    42841 gtatggtgct gatcaaccgt atcctgcctg gctttgaaaa ccgttgtatt gctcttgacg
    42901 atcgttacgg tgcctggctg gcaacgcgtc atttaattca gcaaggtcat acccgcattg
    42961 gttatctgtg ctctaaccac tctatttctg acgccgaaga tcgtctgcaa gggtattacg
    43021 atgcccttgc tgaaagtggt attccggcca atgatcggct ggtgacattt ggcgaaccag
    43081 acgaaagcgg cggcgaacag gcaatgaccg agcttttggg acgaggaaga aatttcactg
    43141 cggtagcctg ttataacgat tcaatggcgg cgggtgcgat gggcgttctc aatgataatg
    43201 gtattgatgt accgggcgaa atttcgttaa ttggctttga tgatgtgctg gtgtcacgct
    43261 atgtgcgacc gcgcctgacc accgtgcgtt atccaatcgt gacgatggcg acccaggctg
    43321 ccgaactggc tttggcgctg gcggataatc gccctctccc ggaaatcact aatgtcttta
    43381 gtccgacgct ggtacgtcgc cattcagtgt caactccgtc gctggaggca agtcatcatg
    43441 caaccagcga ctaaccgcag ttaaagcaat tccagcgcca gtaattcttc gatggtctgg
    43501 cgacggcgaa tcaaccgcgc ctgaccatta tcaaacagaa cttctggtaa cagcggacgg
    43561 ctgttgtagt tggatgacat tgatgcgcca tatgcccctg tatcatgcag taccagataa
    43621 tcacccgcct tcacttccgg caaggcgcgg gtttcgacat ttcccccttc ctgctgggta
    43681 aagacatcgc ccgattcaca taacggcccg gcgacgacgg tttccaccgt tggcgcgtgt
    43741 tccagagaac gaccatcagc ggccagggca ctgatatggt ggtaactacc gtacattgcc
    43801 gggcgcatca gatcgttgaa cccggcatca accagcacaa agtggcggct acccatttgt
    43861 ttgacgctcc gcacctgagt aattaacacg ccagactgcg ctaccagaaa gcgacccggt
    43921 tcaatttcca gtttcacagg gtggcccaaa tggctggcga tttgctgacg cgcggcattc
    43981 cacagaccat aataatgctc ggtatcaacc gcctcttcac cctgttgata aggaatagaa
    44041 agcccaccgc ctgcagaaat agcctgtaaa tcctgaccga attcgatgac ctgacgtacc
    44101 atagcaccac acacctgttc cagatgggca taatcaacgc cagaaccaat gtgcatgtga
    44161 atgccgacca gctgcagatg atgacgttgt atcacgtcca gtgcggcggg cagatcggtg
    44221 taccagatac cgtgcttgct gttttcgcca ccggtattgg ttttttggct atgtccgtga
    44281 ccaaaccccg ggttaacgcg cagccatacc cgatgccctg gcgaaacctg gcccagttgg
    44341 tcgagcatat caacagaacc cgcattcacc ggaatttgca attcactgac gcgttcaagc
    44401 gtcgcccgat cgataacatc tgccgtaaaa acaatatcat cggggtgcgt ttgcggattg
    44461 taacccgccg ccaacgcacg ctctatttcg cctaacgaga cggaatccac tttcacgccc
    44521 tgctcacgca ttaagcgcaa aatatgaata ttggaacagg ctttctgtgc aaagcgcacc
    44581 acatcaaact gtttcagcgc tgcaatctgc cgacgaataa tttgcgcatc gtagacccac
    44641 accgggcagc caaattcagc gggcaaacgc agcagatttt cggcggtgag atcggtatcg
    44701 gtactgaaca gtgaatgtgg cataacaaac tccagata
//
LOCUS       DAZFFG010000023        44272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFFG010000023 DAZFFG010000000
VERSION     DAZFFG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.023"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            5..1771
                     /gene="adeD"
                     /locus_tag="ACHJ7J_001654"
     CDS             5..1771
                     /gene="adeD"
                     /locus_tag="ACHJ7J_001654"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO4999905.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAKQARQNQYLQVSSCVPALEGCD
                     VNGASFPLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1819..3210)
                     /gene="uhpT"
                     /locus_tag="ACHJ7J_001655"
     CDS             complement(1819..3210)
                     /gene="uhpT"
                     /locus_tag="ACHJ7J_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO4999906.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3348..4667)
                     /gene="uhpC"
                     /locus_tag="ACHJ7J_001656"
     CDS             complement(3348..4667)
                     /gene="uhpC"
                     /locus_tag="ACHJ7J_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4999907.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4677..6179)
                     /gene="uhpB"
                     /locus_tag="ACHJ7J_001657"
     CDS             complement(4677..6179)
                     /gene="uhpB"
                     /locus_tag="ACHJ7J_001657"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO4999908.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWMLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6179..6769)
                     /gene="uhpA"
                     /locus_tag="ACHJ7J_001658"
     CDS             complement(6179..6769)
                     /gene="uhpA"
                     /locus_tag="ACHJ7J_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO4999909.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIVAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6933..8369)
                     /locus_tag="ACHJ7J_001659"
     CDS             complement(6933..8369)
                     /locus_tag="ACHJ7J_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999910.1"
                     /translation="MLSIDISNNVKICKVSYCEVNMVSVTSYTYPTETENDNSQFQSQ
                     KLRESINTSLESNISKFNTMEQSGLLDNCRTLSKDATFLEVFRSLLLYILDSFHIYRH
                     DISDKLVPYFHVTPESENANYKTWIAVTGMDSYTKIEQTGSNKFKVTDIYREFNGQTK
                     CFEQKCRDRDFSGRNVEEFIGLLSAHDRLEKDSNSKLTVAADILKQELAINNRDDRYS
                     LQSEAEKIMMGVLKEREGDKLTPEKYLYQTDTKKLTLNEKEFNSLIGDTFRQVSEGSS
                     RILCCGVKINDTIRNLLERKVAELENVKGFQTYNSYEELFNKIIFTNDECQLVRDSLN
                     EDSVSSEKIDDIISSLSTSSGIGNLYYACSQAFHGANDYVHSEVLTLVENVSVLASNY
                     PAFVALNGRNNALFADFSEHTFKQNYYILRAVGNNEAGNFKLANFIYTTETNLLNNNT
                     DAGSVIEIKESSSIKRTFTGNVTIEAFN"
     gene            complement(8585..9880)
                     /locus_tag="ACHJ7J_001660"
     CDS             complement(8585..9880)
                     /locus_tag="ACHJ7J_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999911.1"
                     /translation="MASPINSGIMMSNLCSSTFLDKNINVAAELDITNSEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGENEYTVIE
                     QTGGKSFKVIQFDAGEKNTKKIVKETSYSGVAVATIIKSLSEKTAALETHSADTVLRK
                     KLVNSIVMKNTDCSYEMPADIVSNTYDLLNLRIQKDEGHLPVQEKIDITEDYLHSMTM
                     DAHRCIKEQTKPSGAAKITCCGVELSKDILKKFANKIEKIEQDPKYIQNTLEEMVKQL
                     CFDVNDCAEVRELLRQSNGNITNDKIDEIIACLASPQGITCFYYSSAQGYQSRLQHTS
                     QDVRELLSKSLDGATSSTFHLKKSGNAFFGSGKYLNEMDFTMNGFSQKIYLMAVNKIE
                     NEVKLQCARYFTTGRVDEQNSKIDKVAFTFSATATTADL"
     gene            complement(10143..10433)
                     /gene="ilvN"
                     /locus_tag="ACHJ7J_001661"
     CDS             complement(10143..10433)
                     /gene="ilvN"
                     /locus_tag="ACHJ7J_001661"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4999912.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10437..12125)
                     /gene="ilvB"
                     /locus_tag="ACHJ7J_001662"
     CDS             complement(10437..12125)
                     /gene="ilvB"
                     /locus_tag="ACHJ7J_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO4999913.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPVRVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12231..12329)
                     /gene="ivbL"
                     /locus_tag="ACHJ7J_001663"
     CDS             complement(12231..12329)
                     /gene="ivbL"
                     /locus_tag="ACHJ7J_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO4999914.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            12894..12983
                     /gene="tisB"
                     /locus_tag="ACHJ7J_001664"
     CDS             12894..12983
                     /gene="tisB"
                     /locus_tag="ACHJ7J_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO4999915.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(13107..13181)
                     /locus_tag="ACHJ7J_001665"
     CDS             complement(13107..13181)
                     /locus_tag="ACHJ7J_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999916.1"
                     /translation="MNVSQICARNGEKKNGRICKQKRQ"
     gene            13263..14447
                     /gene="emrD"
                     /locus_tag="ACHJ7J_001666"
     CDS             13263..14447
                     /gene="emrD"
                     /locus_tag="ACHJ7J_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO4999917.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(14455..14952)
                     /gene="yidF"
                     /locus_tag="ACHJ7J_001667"
     CDS             complement(14455..14952)
                     /gene="yidF"
                     /locus_tag="ACHJ7J_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO4999918.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDTLQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDSTLQKWCGSENP
                     HPRRDCQACDWHRLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(14949..15311)
                     /gene="yidG"
                     /locus_tag="ACHJ7J_001668"
     CDS             complement(14949..15311)
                     /gene="yidG"
                     /locus_tag="ACHJ7J_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO4999919.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15301..15648)
                     /gene="yidH"
                     /locus_tag="ACHJ7J_001669"
     CDS             complement(15301..15648)
                     /gene="yidH"
                     /locus_tag="ACHJ7J_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO4999920.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            15757..16206
                     /gene="yidI"
                     /locus_tag="ACHJ7J_001670"
     CDS             15757..16206
                     /gene="yidI"
                     /locus_tag="ACHJ7J_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO4999921.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPKLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16253..17746)
                     /gene="yidJ"
                     /locus_tag="ACHJ7J_001671"
     CDS             complement(16253..17746)
                     /gene="yidJ"
                     /locus_tag="ACHJ7J_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4999922.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARTEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYQLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVNTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEDKKQKF"
     gene            complement(17743..19458)
                     /gene="yidK"
                     /locus_tag="ACHJ7J_001672"
     CDS             complement(17743..19458)
                     /gene="yidK"
                     /locus_tag="ACHJ7J_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO4999923.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            19625..20518
                     /gene="yidL"
                     /locus_tag="ACHJ7J_001673"
     CDS             19625..20518
                     /gene="yidL"
                     /locus_tag="ACHJ7J_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4999924.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20515..21837)
                     /locus_tag="ACHJ7J_001674"
     CDS             complement(20515..21837)
                     /locus_tag="ACHJ7J_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO4999925.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(21837..23453)
                     /locus_tag="ACHJ7J_001675"
     CDS             complement(21837..23453)
                     /locus_tag="ACHJ7J_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4999926.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23749..24465
                     /gene="yidP"
                     /locus_tag="ACHJ7J_001676"
     CDS             23749..24465
                     /gene="yidP"
                     /locus_tag="ACHJ7J_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4999927.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            complement(24462..26123)
                     /gene="yidE"
                     /locus_tag="ACHJ7J_001677"
     CDS             complement(24462..26123)
                     /gene="yidE"
                     /locus_tag="ACHJ7J_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO4999928.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26319..26747)
                     /gene="ibpB"
                     /locus_tag="ACHJ7J_001678"
     CDS             complement(26319..26747)
                     /gene="ibpB"
                     /locus_tag="ACHJ7J_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO4999929.1"
                     /translation="MPNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26859..27272)
                     /gene="ibpA"
                     /locus_tag="ACHJ7J_001679"
     CDS             complement(26859..27272)
                     /gene="ibpA"
                     /locus_tag="ACHJ7J_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO4999930.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27266..27338)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27578..27910
                     /gene="yidQ"
                     /locus_tag="ACHJ7J_001680"
     CDS             27578..27910
                     /gene="yidQ"
                     /locus_tag="ACHJ7J_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4999931.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASASM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27912..29126)
                     /gene="yidR"
                     /locus_tag="ACHJ7J_001681"
     CDS             complement(27912..29126)
                     /gene="yidR"
                     /locus_tag="ACHJ7J_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO4999932.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSDKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGEMVSFTYNDHVMHERSPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCALVSKTTPTPQPGSDEINRAYEEGWVGNQALAF
                     IGDTLSPKSEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAESGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            29227..30291
                     /gene="cbrA"
                     /locus_tag="ACHJ7J_001682"
     CDS             29227..30291
                     /gene="cbrA"
                     /locus_tag="ACHJ7J_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO4999933.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFMTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(30288..30401)
                     /locus_tag="ACHJ7J_001683"
                     /pseudo
     CDS             complement(30288..30401)
                     /locus_tag="ACHJ7J_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418146.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30537..31193
                     /gene="yidX"
                     /locus_tag="ACHJ7J_001684"
     CDS             30537..31193
                     /gene="yidX"
                     /locus_tag="ACHJ7J_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO4999934.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGTSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(31239..32051)
                     /gene="yidA"
                     /locus_tag="ACHJ7J_001685"
     CDS             complement(31239..32051)
                     /gene="yidA"
                     /locus_tag="ACHJ7J_001685"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO4999935.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(32166..32564)
                     /gene="yidB"
                     /locus_tag="ACHJ7J_001686"
     CDS             complement(32166..32564)
                     /gene="yidB"
                     /locus_tag="ACHJ7J_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO4999936.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDNSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            32682..32780
                     /locus_tag="ACHJ7J_001687"
                     /pseudo
     CDS             32682..32780
                     /locus_tag="ACHJ7J_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32804..35218)
                     /gene="gyrB"
                     /locus_tag="ACHJ7J_001688"
     CDS             complement(32804..35218)
                     /gene="gyrB"
                     /locus_tag="ACHJ7J_001688"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO4999937.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSVVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(35247..36320)
                     /gene="recF"
                     /locus_tag="ACHJ7J_001689"
     CDS             complement(35247..36320)
                     /gene="recF"
                     /locus_tag="ACHJ7J_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO4999938.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(36320..37420)
                     /gene="dnaN"
                     /locus_tag="ACHJ7J_001690"
     CDS             complement(36320..37420)
                     /gene="dnaN"
                     /locus_tag="ACHJ7J_001690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO4999939.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(37425..38828)
                     /gene="dnaA"
                     /locus_tag="ACHJ7J_001691"
     CDS             complement(37425..38828)
                     /gene="dnaA"
                     /locus_tag="ACHJ7J_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO4999940.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            39125..39205
                     /locus_tag="ACHJ7J_001692"
     CDS             39125..39205
                     /locus_tag="ACHJ7J_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999941.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            39435..39575
                     /gene="rpmH"
                     /locus_tag="ACHJ7J_001693"
     CDS             39435..39575
                     /gene="rpmH"
                     /locus_tag="ACHJ7J_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO4999942.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            39592..39951
                     /gene="rnpA"
                     /locus_tag="ACHJ7J_001694"
     CDS             39592..39951
                     /gene="rnpA"
                     /locus_tag="ACHJ7J_001694"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO4999943.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            39915..40172
                     /gene="yidD"
                     /locus_tag="ACHJ7J_001695"
     CDS             39915..40172
                     /gene="yidD"
                     /locus_tag="ACHJ7J_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO4999944.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            40175..41821
                     /gene="yidC"
                     /locus_tag="ACHJ7J_001696"
     CDS             40175..41821
                     /gene="yidC"
                     /locus_tag="ACHJ7J_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO4999945.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            41927..43291
                     /gene="mnmE"
                     /locus_tag="ACHJ7J_001697"
     CDS             41927..43291
                     /gene="mnmE"
                     /locus_tag="ACHJ7J_001697"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO4999946.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGISEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(43430..>44272)
                     /locus_tag="ACHJ7J_001698"
     CDS             complement(43430..>44272)
                     /locus_tag="ACHJ7J_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFO4999947.1"
                     /translation="ALRNNQNKIIKAWLLAIDDFEKEFEVNKNEMLLSIVNEIDSTYG
                     LNSAIRTNDYNVVSILLANIKAKMFKNELNKEDILKLMAAREKVAGASDKWTKASGLY
                     SAIVKGHTEIVAAWMETAEVIASHYENDKDVVRELLSLSRNNAVCSLYVASYKTMSKQ
                     VIDVYLNAAIRLALQHGFTFDEIMEQFTRDFDGNSLSLAVEKADDIHMRLWLKIFKVV
                     VGENENYLKDVMMQLEAKNNEGKYVISKANGNPVFKELFWKAIDEFNFPQEELNRLNQ
                     YRSL"
BASE COUNT        10914 a        11651 c        10966 g        10741 t
ORIGIN      
        1 aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg aataccagga
       61 attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata atgtctctat
      121 tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag gacgttacat
      181 tgccggtgtt ggcgcagaat acactgatgc tccggctttg cagcggattg atgcccgcgg
      241 cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca gcatgatgac
      301 gccggtcacc tttgaaaccg ctactctgcc gcgcggcctg acgaccgtta tttgcgaccc
      361 tcatgaaatc gtcaacgtga tgggcgaagc cggattcgcc tggtttgccc gctgtgccaa
      421 acaggcaagg caaaaccagt acttacaggt cagctcttgc gtgcccgctc tggaaggctg
      481 cgatgttaac ggtgccagtt ttccccttga acagatgctc gcctggcggg accatccgca
      541 ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc agaatgcgct
      601 gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc cgggtttggg
      661 tggtaaagaa cttaacgcct atattgctgc gggtattgaa aactgccacg aaagttatca
      721 gctggaagaa ggacggcgga aattacaact cggcatgtcg ttgatgatcc gcgaagggtc
      781 cgctgcccgc aatctcaacg cactggcacc gttgatcaac gaatttaaca gcccgcaatg
      841 catgctctgt accgatgacc gtaacccgtg ggaaatcgct catgaagggc atatcgatgc
      901 cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat atcgcgtcgc
      961 cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg cacccggaaa
     1021 gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc aggtactggt
     1081 gaaaggcgaa ccgattgacg cgcaaacctt acaggcggaa gagtcggcga gactggcaca
     1141 atccgctccg ccgtatggca ataccattgc ccgccagcca gtttccgcca gcgactttgc
     1201 cctgcaattc acgcccggaa aacgctatcg ggtcattgac gtcatccata acgaattgat
     1261 tacgcactcc cgctccagcg tctacagcga aaatggtttt gatcgcgatg atgtgtgctt
     1321 tattgccgta cttgagcgtt acgggcaacg tctggctccg gcctgtggtt tgctcggcgg
     1381 ctttggcctg aatgaaggtg cgctggctgc gacggtcagt catgacagcc ataatattgt
     1441 ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga ttcaggatgg
     1501 cggcgggttg tgcgtggtac gtaacggcca ggtacaaagt catctaccgt tgcccattgc
     1561 cgggctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg ccttgaaagc
     1621 cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg cgtttctttc
     1681 tctgccagtg atccccgcgc tgaaactaac cagtcagggg ctatttgatg gcgagaaatt
     1741 tgccttcact acgctggaag tcacggaata ataaaaaaaa agcccggcgt catgccgggc
     1801 aaaagtcacc agttacgttt atgccactgt caactgctga atttttttct cgcggcggat
     1861 tttgcgttct tccatcaccg ccactatcgc catcaggcag atacaaccaa tcgcggcgat
     1921 atccagcgcg gcgaaggtgc ctgcccagcc ggtaaggccg aataccggcg tcccatcggc
     1981 aatcattccc agacctaact tggcaaagct gtcgccaatc aggtaagcaa aggtgccttt
     2041 aataccatcg gcagcaccaa tcgctttttt aggtacaaaa ccaacagcag ccacaccaat
     2101 caacaattgc gggccaaaga ccaggaaacc caacgcaaag agagaagcca gatagatata
     2161 ttcgttgctg gcatgttgat acacaccgag cgtggcgata atcagcgcca gcgcgatgca
     2221 ggccaccagg ccacggcgac cgttcgccag gtcagagagc cagccccaca gcagcgtacc
     2281 gaccagcgca ccagcttcaa acagcgtaaa gccctgaatc gccaccgctt tagagagttt
     2341 cagttcctgg aacgcgtata cggttgacca ctggtcgata ccaatacgta ccacatagag
     2401 gaaaatgttg gcgaagcaca gcagccagat cactttgttt ttcagcacat actcaacaaa
     2461 gatctgccac ttggtcatat cggtagattc tgtctctttg tcctcttcgc tgatctcctc
     2521 gccgaacagt tcttcagctt tgccgaggcc ataagattcc ggggagtcgc tgccgtaacg
     2581 caggccgata aaaccgacaa tcagcgcgat aatcgacggg aagataaaca tgccgataac
     2641 atgaccatcg aacaggtaat ttgcgccgaa cagcgccaca cctgctgcac ctgcaccgcc
     2701 aaggttgtga gaaatgttcc agaaaccgag gaaggtcccg cgcttacgac gcggcgtcca
     2761 tttggtgatg gtggagtaac tgcacgaacc gccggtgctc tggaaaaagc cgcttaaggc
     2821 gtagaaggca atcatcagga acaggctaac cgagccgctg cccatactgg cgctgaagcc
     2881 cagcatacaa atggcagaca ggatcagcat gaacggcagg aattgtttgg tgtttttgcc
     2941 gtcggcgtag taggaaacca gcgttttacc cacgccataa gtgatggaga aacccaggcc
     3001 gatcatcccc agctgcgtca tgctcaaccc gtaggtcgaa atcatatcgt tctgcgcgat
     3061 gttaaagttc ttgcgaatca ggtacatcgt caggtagccg ataaagacca ccaggtagga
     3121 ttgcatgaac ggtttgaacc acattttgcg ccgcacttcg agcggaaggt ccagggtcgg
     3181 cttgcgaacc tggtttaaga aagccagcat gggttactcc tgaaatgaat acctgcgtga
     3241 ggcatgcatt gtaaaaatca gcagcaaatc aggcctgaga tagcgtccag gcaaaacctg
     3301 gaaaatttct tagttttgcc ggatatgaag tgaaaaggtg agatgcatca cgcttcgcgc
     3361 ggtgtctggg cgttcaaaaa gggcagtaac agcagtgcgg aaatcccggc ggcgatagag
     3421 ataaccacaa aaaatccgct ccagtgccag gtatcgagta ctttcgccag tggccaacca
     3481 gcaagcgacg cccccagata agcaaacaag ccgacaaacc ccgtcgccgc ccctgccgcc
     3541 tctttgtggg aacactctgc cgccgccatg ccgattaaca tctgcgggcc aaagacaaaa
     3601 aaaccaatag tgaagaagca ggttgcctgc atcacgtagc tggcaaatgg catcagccac
     3661 agcgagccga ctgaaagcaa aattccggcg gcgaaaatca aattcatcgg cccacggttg
     3721 ccgttaaaca atttgtccga gccccaaccg gctaccagcg caccgataaa tccgcccagt
     3781 tcaaacatcg tcactgccgt attcgccgtg accagatcga cgcccagcgt ctcggacata
     3841 tacaaattgc cccagtcgtt gattgccgcc cggaccacat agaccagcac atagcaaaac
     3901 gaaagcagcc agatatacgg attcagtaac acatacttgg tgaggatctc tttacgcgtc
     3961 agccctgccc cttcttgttg ttgagcaatc tccagcgcgt cgtgccgcca ttcaccgacc
     4021 gccggtaaac ctaacgcctg cgggcgatcg cgcagccgcc agcagagaaa aatccccacg
     4081 acgatcgcca tacaaccagc aatcatcatc ccggcacgcc agccgtaatg tagtgcagcc
     4141 gctgccatca caatgggaat gagtgcgccg ccgacgttat gcgccgtgtt ccataatgcc
     4201 caccaaccgc cgcgctcggt acgagaatac caggcggtta acagacgcgc acacaccggt
     4261 gaaccccagc cctggaaaaa ggcgttcagc acccagagta cggcaaacgc ccatagcgac
     4321 gtcgagaagc caaacagaat gttgataatg cccgtggcga taagccctat ccccataaaa
     4381 taacgggcat ttgagcgatc gctgacaatg ccggagacaa acttcgacac gccgtaagta
     4441 atgtaaaaca gggtcgctaa caggccgata tcgctacggc tgagcacgcc gttagcaagg
     4501 atttctggta cggcggcgtt aaaacttttc cgtgtgaagt aaaacagcgc gtaacccagc
     4561 cagatggtca gcagaatatg ccgacgccag tagcgatagc gtgcatcaat ttcatgtttg
     4621 tcagtcatta atggcgcatc ggcaggcgct ttcagaaacg gcaacatcat cgaaccttag
     4681 acataacgtt gaggtagaga aacgctgaca cgcgtgccgt gcagacagga aatgtgtaat
     4741 gtgccgccca gcgccgttac gcgctcgcgc attccggtga ggccaaaacc ttgttgcccg
     4801 gaacccggtg gcaaaccgct accatcgtct tcaatcacca gcatcaaccg ttcatcctgc
     4861 tgccagcctt gcagggtgac cgcgctggca tcagcatgtt tcacaatgtt gttcagccct
     4921 tcctggcaga cgcgaaacag cgtcacgcgc tggttttcac ttaacgctga ctcatcgata
     4981 cgccattcga gatggctgac aatgccgcgc ccttccagct ccatttcccg catcagtgag
     5041 cggatggcct gctccagggt gagatcatcc agctggcgcg gacgtaaccg ccctaacaaa
     5101 cggcgcaccg catcgtaaac acccagcgac agttgttcga tgagctgccc gctctgcttc
     5161 acgctggcgt tatctgccgc cagtcgctgg acaatgcccg cctgagtacg aatggcagtg
     5221 atggtctgac cgatatcgtc gtgcagctca cgcgccacat cacggcgcac gctctcttcg
     5281 gtttctaaca aacgttcagc cagatgctga ttgcgcgcca gttccttttg tagcgactgg
     5341 ttaagttcac gcaaccgctg gatgcctgcg cctagtaaca accctgtcag actttgcacc
     5401 agcagcgaga gcaataaatc caccggatga tcgcgccagg tttgactggc gatcagcgcg
     5461 atggcgttca tcaacgtcgc aatcagcgcc ccttgccagc catagtgcca ggccagcgcg
     5521 ataatcggca gcgccaggca gaatggcgta aagcgcgaca gttcgtccgg caatcccaac
     5581 tggagccaga gactaataac aaatagcagc aagtaccaga ccagatgccg cccgcgccag
     5641 ttgatcggtt gagaaactaa cgacgggccg agcggcagcc aggtgttatt ggcgagatag
     5701 tgccagaaca ccagacatat aggggccagc gtcaggccgc cagttaaagt cagtagtagc
     5761 gcattccacg actctttgcc gtgccaaagc cagggcagcg actgcaacaa cgccgccgcc
     5821 gtcagggccg ccccctgtaa cagcaaggtg cgccagtcac gttgatggcg atagcgcgag
     5881 atcagcgcaa cgggtagtaa cgtcagtaaa ctaccgatca tcaataagga aaaatgggtt
     5941 aaaccgaccg cctgcgttag ccagtaaatc agcatccact ccgcgcccag cagcaccggc
     6001 cagtatccgc gcgggcattg cagcattagc cccagacgca gaccaaacgg aaataacagc
     6061 accgccatat cagggcgctc aaccaggtgc aggctgatac tccacaagca aaaccatgcg
     6121 gcagagaaga taaaaaagca ggcaataacg gtaattaagc gggagaacaa cgtcttcatc
     6181 accagccatc aaacatgcgg cgcgccagtt caacgtcgtt actgacaccc agtttttcca
     6241 tcaggttggc gcgatggacg tgtaccgttt tcggtgacaa gcccagttcg gcggcaatct
     6301 ctttcaccgc cattccttgc gccagttttt ccgccacctg gcgttcacgt ttggttagcg
     6361 ggtcctggcg accggatgcc agtttaatgg caatatccgg cgtcagataa cagccgcccg
     6421 tggcaaccgt atgcaccgca acaatcagtt cgtcagggct acaacgtttg gagagaaagc
     6481 cgcgtgcccc cgcgttaagc gcctgctcaa ccagcgcagg actgtcgtga acggagagca
     6541 taattgtcgc catacctttc ggcagctggc ttagcaactc cagaccggag atatcgggca
     6601 tggagatatc gcaaatacac acctgcacac cgcgccccgg cagccccgcc agcgcctcgc
     6661 gccccgaacc aaactcggca acgacttgca aatcaggttc cagccccagc aactgcgcaa
     6721 agccggagcg gacgatgagg tgatcgtcta taagggcaac ggtgatcata attgggtcct
     6781 ggcggataaa aaacgcgcat accttaacga taagcgcgat gttgttcaag ttggatatgg
     6841 agtatgtatt ggcttaatga tgttgggctt atcgtcaaaa cgcctacctg agaggtaagc
     6901 gtttggtgcg tgttgatttt agtcgatgtt aattaattga aggcttctat cgttacattg
     6961 cctgtgaacg ttcgctttat actggaagat tctttaattt caataacaga acccgcatca
     7021 gtattattat tcaggagatt tgtctccgtt gtatagataa aattagccaa tttaaaattg
     7081 ccagcctcat tatttcctac tgctctcaga atatagtaat tttgtttaaa tgtatgttct
     7141 gaaaaatcag caaatagagc gttatttcta ccattcaaag caacaaaagc cgggtagttt
     7201 gacgcaagaa cactaacgtt ttcaacgagt gtaagcacct cggaatgcac atagtcgttc
     7261 gcaccatgaa atgcttgtga acaggcgtaa tacaaattac caataccaga actcgtggat
     7321 aaagatgaaa tgatgtcatc tatcttttct gaagaaacag aatcttcatt tagagagtct
     7381 cttaccaatt ggcattcgtc attagtgaaa atgattttat taaatagttc ttcataacta
     7441 ttataggtct gaaaaccctt gacattttct aattcagcca ccttacgctc aagtaagtta
     7501 cggattgtat catttatttt tactccgcaa cataaaatgc gtgaactgcc ttcgctgact
     7561 tgtctgaatg tatcaccaat aagtgagtta aattcttttt cattaagcgt caattttttt
     7621 gtatctgtct gataaaggta tttttcaggg gttaatttat ctccttccct ttcttttaaa
     7681 acacccatca ttattttttc agcctctgac tgtaaagaat acctatcatc cctattattt
     7741 atagcaagtt cctgttttaa aatatcggca gctactgtta actttgagtt tgaatctttt
     7801 tctaatcgat cgtgcgctga taataaccct ataaactctt ccacatttcg accactaaaa
     7861 tctctatccc tacacttttg ttcaaagcat tttgtttggc cattgaactc acgatatata
     7921 tcagtgactt taaatttatt acttccggtc tgctcaattt ttgtatagct atccatgcct
     7981 gtaacggcaa tccatgtctt ataattcgca ttctccgatt caggcgtaac atgaaaataa
     8041 ggaactaatt tatcactaat atcatgccga tagatatgaa aagaatctaa aatgtataat
     8101 aataaacttc gaaaaacttc aagaaaagtg gcatctttag ataatgttct acaattatcg
     8161 agcaagccac tttgttccat cgtattaaac ttagaaatat tactctcgag gcttgtgttg
     8221 atactctccc ttagcttctg actctgaaac tggctattat cattctctgt ttccgttgga
     8281 tatgtataac tcgtcacact aaccatatta acctcgcaat atgacacttt gcatattttg
     8341 acattgttag aaatatcaat ggatagcatg ataaatattt caatgctaac cgccatattt
     8401 catttatttc aataataaga ctatagtgcc gttaattttt atcgattttc ccaatgcaat
     8461 tgcatcacca atacaattta ttcactatca aaaatcgctc attttttcaa aaatgactgt
     8521 ttacacacag acggttaaga atacgcgctc accttaatga caaacgcgct ttatgaagaa
     8581 aaaatcataa atcagctgtt gttgcggtag cggaaaatgt aaaagcgact ttatcgattt
     8641 tagagttttg ttcgtcgacc ctaccggtag taaaataacg agcacactga agcttaactt
     8701 cattttcaat tttattaacc gccatcaaat atattttctg actaaaccca ttcatagtga
     8761 aatccatttc attaagatac tttccagacc caaaaaatgc atttcctgat ttttttaaat
     8821 gaaatgttga tgaagtggca ccatccaaac tctttgagag taactcgcga acatcttgag
     8881 atgtgtgttg taagcgactc tgatagcctt gtgcggaaga ataataaaaa catgtaatcc
     8941 cctgaggact ggctaagcaa gctattattt catcaatctt atcattcgta atattaccgt
     9001 ttgattgccg taataattct cttacctcag cacaatcatt aacatcaaag caaagctgtt
     9061 taaccatttc ttcaagtgtg ttttgaatat attttggatc ttgttcaatt ttttcaattt
     9121 tgtttgcaaa ctttttaaga atatctttac ttaactcaac accacagcaa gttatttttg
     9181 cagcaccact tggtttagtt tgttctttta tacatctatg agcatccata gtcatagagt
     9241 gtaaataatc ctcagtaata tcaatttttt cctgaacagg taaatgcccc tcatcttttt
     9301 gaattcgcag gtttaataaa tcatatgtat tactcacaat gtctgcaggc atttcgtaac
     9361 tacaatccgt attcttcata acaatactat taactaattt tttcctcaaa accgtgtcag
     9421 cagaatgtgt ttctaacgca gcagtttttt cagacagcga tttgataata gtcgccacag
     9481 ccactccaga atatgatgtt tcttttacta ttttttttgt atttttttca cctgcatcaa
     9541 actgtataac tttaaaggat ttgccacctg tttgttcgat tacggtatat tcgttctctc
     9601 cagttgccgc catccaggta cgattgccat tattttcagt gcctggcata gggtggaaat
     9661 agttaaggat ttcggtgtta acactgcgag aaccggacat aatatttgct attaagcgct
     9721 ttatttcctg caacatccat tttgcaaaat tactgccggg cgaatatttt ttctcgctat
     9781 ttgtaatatc aagctcagca gcgacattta tatttttatc caaaaaggtg gaagagcaga
     9841 gatttgacat cattattccg ctgtttatag ggcttgccat aatatacctc acgatgaacc
     9901 attaattaat agaatgctgg tcatgaccat tggtatagac agcaatcaga atttaacacc
     9961 atcgctggaa attttagccc taactgtgaa cccccaaatt actggtgcag cgacttaaat
    10021 taacattcag ggctaataaa ttgtgcctga gatggtctct ggcacaattt caccctatat
    10081 atctgaacgg atctatcaaa aatcgctcat cagtaccccc ccgagcggtt taatgtcgct
    10141 ccttactgaa aaaacaccgc aatcttgtta aacatcgtcg gatcggactg attacgctgc
    10201 actttcacca catcttccag cttgtctatc tggcttatca tctgctccag acgctggtcg
    10261 tcattgacca gtagccaaat gtggcttttg tcgctgtcct gaatcggcaa gcaaagaatg
    10321 ccctcaacgt taaaagcgcg acgggcaaaa aggccacaaa cgtgggtcat tacgcccgga
    10381 tggttgcgaa cggtgagttc cagaatcacg ttgtcatgag ttgtgttttg catggcttat
    10441 tcccccacca tttcagtatt cgccgcacct ggcggcacca tcgggtacac tttttcttcg
    10501 gcatcaatac gcacatggat cagcgccgga ccagggcgat tgatgatttc ttgcaatgca
    10561 gcctgcggat cggcttcgtt attcaaatca caggtttcga ggccgaatcc ggcggcaatc
    10621 tgcataaagt tgatttttcc cggataggta gcggcaaaaa cgccctgctt gtagaacaga
    10681 ctctgttgct gatgcaccag ccccagcgct tcgttgttca tcagaatgat tttgacatcc
    10741 agctggtttt cactggcggt cgccatctcc tgaatattca tcatcaggct gccgtcgccg
    10801 gagaaacaca acactttgcg atccgggttc gccaacgccg cgccaatcgc cgcaggcagg
    10861 ccaaagccca tcgtgcccag cccgccagag gtcagccact ggcgtgggcg attgagcgga
    10921 taggcttgcg cggtccacat ctgatgctga ccaacgtcgg tggtgataat cgcattgtca
    10981 tcgacacagg cggcaacggc gttgatcagg ccgtaatggc ttaatggatc gcacgctttc
    11041 gggattggac acggaaattc gcgctgcaaa tccgccacca actggtgcca ctctgcacgc
    11101 ggttgcgctt ccaccagcgg gatcaactgc gccagcacgt catcaacatc cgcctgaatc
    11161 gccacatgcg gttgcttgat tttacccagc tctgcgcggt cgatatcgac atgaataatt
    11221 ttggcattcg ggcagaactg ctcggttttg ccaatcgccc ggtcatcaaa acgcgcaccg
    11281 agcacaatca acaaatccgc ctcctgcaaa atatagttgg tgctgcgcac gccgtgcatc
    11341 cccagcatac ccagcgacag cggatgcgct tttggcagca tacccagcgc cattaaggtc
    11401 atggtagttg gcagttgcgc tttctccgcc agttcacgca cccgcacagg cgcattgatc
    11461 acaccgccgc ccagataaag caccgggcgt ttggcggcgt taatcatcgc agccgcgtca
    11521 cgaatgcttt cttcgctaaa ggcgggtgca gcggcttttt ctgccaccgc gggctgagct
    11581 tcaatctcaa aaactgccgt ttgtacatcc ttaggaatgt ctatccacac cgggccaggg
    11641 cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct gcgggagttc ttcgatatgt
    11701 ctgaccagat agttgtgttt ggtgatgggg atagagatgc cgtaggtatc gacttcctgg
    11761 aaggcgtcgg tgccgatcat cgaggcggga acctgaccag tgatgcaaat cagcgggatg
    11821 gagtccagcc gcgcatcggc aatggcggtc accaggttag tcgcacccgg cccgctacag
    11881 gccatacaga ccgccggttt accgtcggtg cgcgccattc cctgagcgat aaaccccgcg
    11941 ccctgttcat ggcgagccag aatatggcgg atttgcgtgc tttggcttaa ggcatcgtaa
    12001 acaggcagga tagaaccgcc cggaatgccc gtcacaatct taatgccctg ctgttccagg
    12061 aaatgaacga taaattctgc gccggtaaag cgcttacgcg tcgatgttgt gcccgaactt
    12121 gccatgctcc agtccttttt ttctgggccg actttccggg aggtgcttaa acgaaaaacc
    12181 ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt gttccagtcc ctacggcgca
    12241 ttgccgacga ccaccaccac acgcacgacg accactgcgg cggatggcgc agttggtagt
    12301 agctttgcgt tgaacatgga agtagtcatt ggggaccttg ttggttttgt gtttaacaat
    12361 atttatacaa gcacagcttt acaggggaga caatggaaaa tttttcagca agggaaaatt
    12421 gaggggttga tcacgtttta tactgaattg caggtaacaa aaaaccccgc cggagcgagg
    12481 tttcgtcagc tgcctgcggc tggtaaccgc aaagcacact gtattatgtc aacactgaaa
    12541 gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa ttttgacgta tttagtgcat
    12601 agttgagtat cgatcacagt ttgcgttttg tccaaatatt actgtttatt tatacagtaa
    12661 acttctataa tatcactgta cgcaatgtgt tatgcggggg ccgcatcgtt acccggcgca
    12721 ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta accccgcgtg agcacactgt
    12781 gttatgtcaa caagcacaac gtttctcctt gagataccgc gtgcacaaca gctggcaaca
    12841 ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc acaggagacg cgtatgagcc
    12901 tggtggatat cgccattctt atcctcaaac tcattgttgc agcactgcaa ctacttgatg
    12961 ctgttctgaa atacctgaag taattcagat tcaagtcgca ccaaagggga gcgggaaacc
    13021 gctccccttt tatattctgc ttgtgggttg gtgtcggatg cgatgctgac gcatcttatc
    13081 cgacttacca tcccttccgg caacatttat tgccgctttt gtttacatat tctgccgttt
    13141 ttcttttcgc cattcctggc acatatctgg ctaacattca tcaatgtgat agattcctct
    13201 cccgcattta tgggaatgcg tagtgactta ttctaattat ttttataaaa gcatccgtga
    13261 taatgaaaag gcatagaaac gtcaatttgt tattgatgtt ggtattactc gtggccgtcg
    13321 gtcagatggc gcaaaccatt tatattccag ctattgccga tatggcgcgc gatctcaacg
    13381 tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct gctgacttac ggtgtctcac
    13441 agctgtttta tggcccgatt tccgaccgtg tgggtcgccg accggtgatc ctcgtcggaa
    13501 tgtccatttt tatgctggca acgctggtcg cggtcacgac ctccagtttg acggtattga
    13561 ttgccgccag cgcgatgcag gggatgggca ccggcgttgg cggcgtaatg gcgcgtactt
    13621 tgccgcgtga tttatatgaa cggacacagt tgcgccatgc taacagcctg ttaaacatgg
    13681 ggattcttgt cagtccgttg ctcgcaccgc taatcggcgg tctgctagat acgatgtgga
    13741 actggcgcgc ctgttatctc tttttgttgg tcctttgtgc tggtgtgacc ttcagtatgg
    13801 cccgctggat gccggaaacg cgtccggtcg acgcaccccg cacgcgcctg cttaccagtt
    13861 ataaaacgct tttcggtaac agcggtttta actgttattt gctgatgctg attggcggtc
    13921 tggccgggat tgccgccttt gaagcctgct ccggcgtgct gatgggcgcg gtgttagggc
    13981 tgagcagtat gacggtcagt attttgttta ttctgccgat tccggcggca ttttttgggg
    14041 catggtttgc cgggcgtcct aataaacgct tctcaacgtt gatgtggcag tcggttatct
    14101 gctgcctgct ggctggcttg ctgatgtgga tccccgactg gtttggcgtg atgaatgtct
    14161 ggacgctgct tgttcccgcc gcgctgttct ttttcggtgc cgggatgctg tttccgctgg
    14221 cgaccagcgg cgcgatggag ccgttcccct tcctggcggg cacggctggc gcgctggtcg
    14281 gcggtctgca aaacattggt tccggcgtgc tggcgtcgct ctcagcgatg ttgccgcaaa
    14341 ccggtcaggg cagcctgggg ttgttgatga ccttaatggg attgttgatc gtgctgtgct
    14401 ggctaccgct ggcaacgcgg atgtcgcatc aggggcagcc cgtttaagcg cacatcaccg
    14461 cagcatcgtc atcagctcca tgggagaacg atgctgcttt atcagatcac gcatcacccg
    14521 catatgcggt gcggagtaag aataaaacgc ctgatagccc gcacatagca cgctgtctgg
    14581 cgtttcctcc cgagcgtgcg gacacagccg atgccagtcg cacgcctggc agtcacggcg
    14641 cgggtgcggg ttttcgctgc cacaccattt ttgtagggtg gagtcaaata gctcaatcga
    14701 gatacgactg atatctgccc gcacccaaca ctcgaacagg gcaattaaaa atttccccca
    14761 cagctctgct gtcaccgaag cggccgtcat atcgccccgg tcatcaaatt caagatgggg
    14821 aataagctgt acggagcgga tgccccgctg tttgaaattg tgataaaaat cggcaggttg
    14881 cagagtatcc ttatactcta caaccagttt atgcctgtcc tcttcagcat caatgacctg
    14941 acttcctgtc atgcgactct ctcaataaat acgataagtt gatgtatatg cgttacagca
    15001 aacagtattg cgagagataa caccgcgagg gagatcaaaa atttgtcacg gactacgtga
    15061 aattgggaaa aatcgctatt cgtgacatcc attaaattgc gattacgagt gtagtgccag
    15121 aggatcagcg ccacgatggc gaggatgcca atcgaaatcc agaataacat gcccgcctgg
    15181 tgccagttgt gtttgatagc caacgccatc aacgcgccgt agcccagcat ggtacgaaac
    15241 cacgccaatg atgtacgctc cggctgcagc cctgggtcgg caatgcgtct ggctttgcgg
    15301 ctatccggca tacaacacca gtcccataac gatcactgct acgaccatca atattaagct
    15361 gatgattaat aagctgttgg tgtagggcag gtcctctttc agacgcatcg ctttttcatt
    15421 gcgtaaccag cgcaagtagc catacatcgc taacccgccg gaaaacagac acaacaacaa
    15481 cgccagcagt tcgcgaatga cgggcgtggc gaaatctggc gcaagttgat caagcccaac
    15541 ccccgccgcc agaaatccta gtgcagtacg gatccacgcc agaaaagtac gctcattagc
    15601 cagcgagaag cggtaatcgg gcgcttctcc gaggcgggaa attttcattg ttgctccttt
    15661 gcaatttcac taacgctcta cctttagcgg ttttttttaa gaatttcgtc cgcccgtttc
    15721 ctgatatgct tttgccatcc gctacaagga ggtcagatgg gtatcatagc gcaaaataaa
    15781 atcagttcat tgggcatgct attcggtgcc atcgctttaa tgatggggat tatccacttt
    15841 tcattcggcc ctttctccgc accaccgcca aagcttgaaa gcatcgtggc ggacaaaacg
    15901 gctgaaatta aaagaggtct tctcgccgga ataaaaggcg aaaaaattac caccgtggag
    15961 aaaaaagagg acgtagatgt cgataaaatc ctcgatcaaa gcggtatcgc actggccatt
    16021 gctgcgctac tttgcgcatt tattggcggt atgcgtaagg aaaatcgctg gggcattcgc
    16081 ggcgcgctgg tttttggcgg cgggacgctg gctttccata ccttgctttt tggtatcggc
    16141 atcgtgtgca gcattttgct gatattcctt atcttttcct tcctgaccgg cggttcactg
    16201 gtttaagcag cctgaataat gcccgccggg tgatcatcag cgggcattat cgtcagaact
    16261 tctgtttttt atcctccact ttcacccctt gtgtcggcag gccggtgtca tagtcacgta
    16321 ccactggcga atagccatct tgtgggcgtg gacgaaacgc ccccatccag cgcggccgtg
    16381 catctttacg ccacggacgc aggctccatt ggtagctgcg gaacggatcg cgaattttgt
    16441 ccatataatc caataaggca tcatgcattt tgctgcgaac gtcggcaaaa tgggcatcat
    16501 cgatcaggtt atgcatttca tttgggtcat tacggcgatc gtaaagttca tcactggtga
    16561 agaggttgag caccagttta aagtcatccg tcacccagca acgcaccgga ataaaaccgc
    16621 caaagctgtc atgctcaatc tcgtagcggt taaattccac catcacgccg cgtggctctt
    16681 tcacggcaag gatattttcc cccggcagaa tctctggttt ttcaatatct gccagcgcca
    16741 tcattgtcgg cagtaaatcg atatgactga ctggcgtatt gacctgtcgc cgctcccctt
    16801 gcggcgaacg gatgatcagc ggaatacggg tgatgtcgtc atacatcgcc gctcctttac
    16861 tgatcagctt atgtgcgccc atcatttcgc cgtgatcgga tgtataaata acccacgtat
    16921 tttcacgttg ctctggcgtt aaggcattga tgacccgtcc tatttggtca tcaacaaagt
    16981 cattacaggc aaaatagagc ggatggtgat aaagcccgtc atcaccgact ggcgatggca
    17041 tcgcctgcgc ccataagcgg tgatgttctg gtttgttcgc caggtcatcc tgtgctttct
    17101 cgcccagttg gtagtaaaaa tcagcgtatt tctctaaata ctccaccgga caggtgaacg
    17161 ggtgatgcgg ctcatcatac gaaaccacca tcaggaaggg ttcctcggtg cgcgcgggct
    17221 gttgcagaaa atccaccgcc cgattgctga tgcggtgcgc ccaagtgaag gtttcgtcaa
    17281 tatggttcgc ctgtaaatct tcgacgctgt ttaggccatt gcgccacagg ctaatctctt
    17341 tttccgtcag ttcgctaaga tagttcgccc catcgaacca gtaatcagcg tcccactccg
    17401 gcggacactc gccagtgccg aaatagtcat gaccgtcgag atgccatttg ccgatgtaac
    17461 aggtgtgata gcccgcatcc ttaaagtagc gccccatagt ggaaatgttt ttgcctggcg
    17521 cgacgttgtt ggtccacggg ccggactggt tagcgtagat accggtaaac agcccggcgc
    17581 gcgccggtgt acaaaccggt gaacaggtgt aggcggagtt aaagcgaata ccttccgccg
    17641 ccagactatc aatattttgc gtattcagcg gcttaccgct atagcaaccg accatattag
    17701 tggcctgggt atcggtcatg atgaacagaa aattggggcg tttcataggc tatcctttgc
    17761 atcgggagta aaggttacgg ctgggtcgtg acgatgccgc cagctgtcaa aaatcaggta
    17821 gacaatcact acggcggcaa tgaaataact gatcatcgct aaccagcgcg taccgtagcc
    17881 gccgaattca gccagcccgg catagacgcc aatcatcgcg aacaggatgc caattgacgc
    17941 gatcttgacg tttttccacg gtttcatgtc caccgcaaac gcatctttga aggtgaacgg
    18001 cgtggcgcgc ggtttgataa aaccgatcac cagcatcacg accacgttga tgcagaacgt
    18061 acaggccagc acatagagga aatggaagtc gaacttcacc agatagttga tggtgatgta
    18121 gctgattatg ccaatcccca tcgccacttt tgccgccagc gccgggatgc gcgggaagaa
    18181 aaagcccata atgatgatgg taaccagcgg cacgttgtaa atgccgttga gctgtttcat
    18241 ccagctatac agcccctgcg gcgcgttggc gatccacggc gctaccagca ccgaaacgat
    18301 agcgataaag aaaccaaatt tgcgcccgac ggtgaccagc tgctgcggct cggcattctg
    18361 gttaatgatg cgacggtaaa tacccatact gaataacgta ctggcgctat tcagaaagcc
    18421 gttgaaggta ctgatcaccg caccaaataa caccgctccg aagaaaccca ccagtggcac
    18481 tggcagaacg ttattgacca gcgtcgggta ggccatgtcg gctttcggta gatcctgata
    18541 cagatgaaac gcaatcaacc ctggcagcac cagtaccagc gggtcgagca ttttcagcac
    18601 cgccgttaac agcgccccct tttgcccttc cgccaggctt tttgacgcca gcgtgcgttg
    18661 cacgatgccc tgatttgtac accagtaaaa ggtgttcacc agaattaaac cggtaaatgc
    18721 cgcgccaatc ggcaagggat cggtcgggcc accgactgag tttaatttct cggcgtgaac
    18781 ggtggtaatt tgctcaatgc cctgcataaa gctgcccttg cccatcgcga tcaggccaaa
    18841 taccggcacc atcaacccgc cgataaccag cccaataccg ttgatggagt ccgccactgc
    18901 cattgcgcgc agtccgccga tcaccgcata caaaattccc gccagaccaa gcaaaattac
    18961 cagtagccag atagccgcac cgtgagaaat ctgtagcgat tccccgacgt gaaacaggct
    19021 gttgagcgcc aacgcgccgg agtagagaac aatcggcaga aagcagacgc cggtggcaat
    19081 gaggaagcag aagtcgatga taatacgcgt cgttttatca taacgttcct ccagaaaatc
    19141 ggggatggtg gcaatgccgc gcttcagata gcgcggtaaa aagattagcg cgaggaagat
    19201 cagcgtcacc gctgaagtga cttcccagcc catcaccgac atgccgcttt tgtaggcctg
    19261 cccggaaaga ccaaccagtt gttccgtgga aaggttggtt aacattaacg aagcggcaat
    19321 aaccggcgct tttagtgaac gtccggcaag aaaatagcct tgttgcgatc cggtatctgt
    19381 tttgcgaacc ttccaccagg tgatgatcgc caccagcagc gtaaaaccga caaaactcaa
    19441 gatttgtaac gaattcatct catagccctt atttatgttt atgtcccgga cgcatgaata
    19501 tagggagagg taaaatgcgt cgggtcaggc agtcagattc ttaaaataca aacgtcgtat
    19561 ccctgaacgg attgttttct gttaagttca ggttgtgagc ataattcgcc ggagggatat
    19621 taaaatgaat ggaaaattgc aaacttcaga tgtaaaaaac gaaactccat acaatattcc
    19681 cttattgatt aacgaaaatg tgatctccag cggaatttct ctgatctcgc tctggcatac
    19741 ctacgccgac gagcattacc gggtgatctg gccgcgggac aagaagaaac cgcttatcgc
    19801 caactcatgg gttgcggtgt ataccgtaca aggatgtggg aaaattcttt taaagaatgg
    19861 cgaacaaata actctgcatg gcaactgtat tatattttta aagccaatgg atattcactc
    19921 ttatcactgt gaaggtttag tctgggaaca gtactggatg gaatttaccc ccaccagtat
    19981 gatggatatt cccgttggtc agcaaagcgt tatttataat ggcgaaattt ataatcagga
    20041 actcaccgaa gttgctgagt taataacttc accagaagca ataaaaaata atctggcagt
    20101 cgcttttctg acgaaaatta tttatcagtg gatttgtctt atgtacgcag acggtaaaaa
    20161 agatccacaa cggcggcaaa ttgaaaaatt aattgccact ttacatgcca gtctgcaaca
    20221 acgctggagc gtagccgata tggctgccac gatcccctgt agcgaagcct ggttgcgtcg
    20281 tctgttttta cgctataccg gcaagacgcc gaaagaatat tacctcgatg cgcgtctgga
    20341 tctggcgcta tcgctattaa agcaacaagg aaactcagtt ggcgaagtcg ctgatacgct
    20401 caacttcttc gactcctttc atttcagcaa agcctttaaa cataaatttg gttatgcgcc
    20461 ttcagccgtg ctaaagaata cggaccagca cccaacggat gccagtccac acaattaacg
    20521 taactctggc cagtacgctt tgttggcctc cagcaactca tccagaatag ctttggcgac
    20581 cgaggcgctc ggtaccgttt tcgacaacgt aatcgcctgc cacaggtgct gatatgaacg
    20641 ctgttcccag gcgtccacga ccagtttttc caccgccact tgctgactca ttaacccttt
    20701 ctgaaactgc gggatatcgc cgaccaccag tggttctggt ccgttgtgcc cgaccagaca
    20761 cgggatctcg accatcgctt catcatcaaa gttatggatt gccccgttgt taggcacaat
    20821 cagcaacatc cgctcctgag tgttgaaggc aattgccgcc gccagatcga cgatgtatga
    20881 cgcatgttcg tcaatctcca gcttgcccgc cgctgaattt cctgccgccg taatggcgcg
    20941 gcaagcatcg aaaacctgtt tctcgcgatg ttccatcacc tcattcgcgc gggtatgttc
    21001 agggttggaa tgctgaacga cataatccgg gaagagataa tatttcagat aggtgtttgg
    21061 taatgtatcc gggtccagtg cctggacatc ccgcgctttt gcataggtgt cgttccagct
    21121 cgcttcagta tgtgtatctt gctgcggtgg aacataaccg tgcttcgaga catattgccg
    21181 cagctggggc atcaggtcgt tgccctgcaa atcttcaatt gatgtccacc agccaaagtg
    21241 gttcaggccg tagtagcgca cgcgcatctg tttgcgatct tgcagcccaa caatttgcgc
    21301 catccggctt tcaataccga ttggcatgtc acagatgttg aggattttcg cattcgggcg
    21361 cagacgacgc gtggcttctg cgacaatggc tgccgggttg gagtagttga gcatccaggc
    21421 gtttggtgaa tatttttcca tataatccac cagttccagg acgccgccaa tggaacgcat
    21481 gccgtacgct attccgccgg gtccgcaagt ttcctgacca acaacgccgt ggcgcagcgg
    21541 gattttttca tccagttcgc gcatcgggta tttgccgacg cggatgtgcg ccatgacaaa
    21601 atcaacgtcg ctgaacgcca cttcaggatc ggtggtgtaa ctaaacgcaa tgtccggcgc
    21661 tttctcttta aggatgactt tacaggcttc ggcaatcact tcctgccgcg cgccatcgtt
    21721 atcataaaat ttcagtgcac gaagcgggaa acggtcctga ttcgccagga gcatcaacac
    21781 gatgcctggc gtaaaggtgc ttccaccgcc tgcgacaacc actgagaatt tggtcattat
    21841 actgcctccg taatggcaac attttctgct gattgatgag aattaattaa gctatcgagc
    21901 tgttcacgca gctgggatac atgcagacca atgatcacct gaatggcatc gccactacgg
    21961 aagacgccgt gcgctcccag ctttttaaag acttcgtcat ccagcgtttg tgacatgtca
    22021 tgcagtgcaa tacgtagacg cgtcgcgcaa ttgttgatgc tggagatatt gccgaccccg
    22081 cccagggctt gcaggatacc ggcagcctga tccagctctt tttttggctc tgccgcggtg
    22141 gtttggcctc gcgaggcttt gtattcggct tttgagtaga gtttcacttc cgcatcttca
    22201 cgtcccggcg tgcacatgtt gaattgcaga atcagggtgc ggaaaaccac gaagtacagc
    22261 agggtaaagc acaacccaat ggcgatttgg gtcaacatca tatccgcgtg gttgctgaac
    22321 atcgggatcc agttttgcgg taaaacctgg tcaatcagac ctccgcccat gttgcccacc
    22381 acaccaaaga gatacatcac ggtcgacatt gaggccgcaa gcacagcgtg taccgcaaac
    22441 agcaacggtg aaatgaacag gaaggtaaat tccagcggtt cggtaattcc caccagcatg
    22501 gcggttaagg ttgcgggaat cagcaagccc gccactttta cccgattttc cggtgcggca
    22561 gtgaagtaca tcgctaaaga aatgcccacg gcaccaaaga tttttgagtt accgtgcagg
    22621 gcaaaaccgc cttccgggaa caacgatttc agcggctcgg cgctcaaact gaactcttgc
    22681 agatgctgcg cccagtacat ctgaatgccg ccttcaacag ctgccggacc aaagataaac
    22741 ggtccgtaga tgaagtggtg taaaccggtt gggatcagaa tacgttcgag gaaggtgtaa
    22801 acccacaccc caagcgcacc cgccgaacgc aggaacgctt gcagagattc aatccccatt
    22861 tgtacttttg gccagccgag caatgtcagc caggcacagg ggatcatcac caggaaggcg
    22921 ataatcacca cataagacgt cccctggaaa atgccgagaa acaccggcag ttttttatcg
    22981 aacagacggt tatgcagcgc cgtcacaatg ccggaaatga taattgcgcc gataatgctg
    23041 gtatcgaggg ttttaatccc ggccatcatt gtcagaccgc tacctgccac cgcgtcctga
    23101 gtgaaatcga cgccgaagta acttccccag gtcattccca tcgcattgat gaaatagttc
    23161 caggtcagga aactcaccat caccgccaga caagcacgcc cctgcgcttg cttagcaagg
    23221 ccaatgggta aaccgacagc aaaaatcagc ggcatattac ggaataccgt ccaaccgccc
    23281 tcttcaataa tgtgtacgat ttgcgcgaat aaactgtttg gatcggtcag tgattccccg
    23341 acaaacatcg ggttttgcag caagatggca agacccacca caatcccggc gaagggaaac
    23401 agcagcactg gcgtgaacat cgcgccgcca aagcgttgaa tttgactgag catttccaca
    23461 tcctcttttc tcaattctgt aagtaggaag cctgattggt tatgcggcag gcctggagcg
    23521 aggataggaa gatgcgatcg gcagaacatg acgacccaat gggattcgtg atcgctttca
    23581 tgcttttcgt tgggcggtag agatctacgt tttcgcgaaa aagtagacct ctgtgagcac
    23641 aggtacacag catcgaacac atctttaaaa aaaagatgtt ttttcaatcg attaagcaga
    23701 acttgtgggc gcattacccg ggcttgcagg caaaaaagag atctagagat gatctacaaa
    23761 agcattgcgg agcggttaag aattagactt aattccgcag atttcacgct aaacagcctt
    23821 cttcccggtg aaaaaaagct ggcagaagag tttgcggtat cgcggatgac catccgtaaa
    23881 gcgattgacc tgctggtagc gtgggggctg gtggtccgcc gccacggcag cggcacttac
    23941 ctggtgcgca aagatgtgct gcatcaaacc gccagcctga ccgggctggt ggaggtgtta
    24001 aaacggcagg gaaaaacagt caccagccag gtgctgattt ttgaaatcat gcctgcgcct
    24061 ccggcaattg ccagccagtt gcggattcaa atcaacgagc agatctactt ctcccgtcgc
    24121 gttcgttttg tggaagggaa accgctgatg ctggaagaca gctatatgcc ggtaaaactg
    24181 ttccgtaacc tttcgttgca acatctggaa gggtcgaagt ttgaatatat tgaacaagag
    24241 tgcgggattt tgattggcgg taattatgaa agcctgacgc cggtgctcgc cgatagactg
    24301 ctggcgcggc aaatgaaggt agcggaacac acgccactgc tgcggatcac ctcgttgtca
    24361 tatagcgaga gcggggagtt tttgaattat tcagtgatgt tcagaaatgc cagcgaatac
    24421 caggtggagt accatttacg gcgactccac ccggacaaga gttaaccgat actccagaag
    24481 agaaccgcca gtaattgggg ggtgataatg cgcaggaaca tcactaacgg atagacagtg
    24541 gcgtaagaga gcgccgccgc accgctggtt ggatgaagat tattagcaaa cgccagcgcc
    24601 ggaggatcgg tcatggaacc tgccagcatc ccgcacatgg tcaggtaatt cattttggct
    24661 aacatccgcg ccagaatgcc aacagtaatc agcggaacgg cggtgatcag ggcaccataa
    24721 ccaatccagc ttagcccttc gccattgacc agggtattca caaaatctcc accagatttc
    24781 agaccaacga ccgagaggaa cagcacgatc cccagttccc gcagcgcgag gttggcgctt
    24841 ggcggcataa accagtacag cttgccgata ctgccgatac gcccgaggat caacgccata
    24901 atcagcggac cgcccgccag ccccagtttc aacgcggccg ggaatcctgg cacaaagacg
    24961 ggaatagaac ctaacaatac gccaagcccg atgccaataa acactggcag catctgaacc
    25021 tgttgcagtt tttgctgcgc attccccagc acattggcaa cggcatcaat tgcggacgga
    25081 cgccccacca ggttgaggat atcgccgaac tgcaggctga tatcgccact ggcgaccagt
    25141 tcgaccccgg cacggttcag gcgcgagata acaacgtcat agcgttcttt aaaatgcagg
    25201 tcgcgaatac gcttaccaag cacgttttca ttggtgacca ccacacgctc gacgcgcaaa
    25261 tcagtgcctt tcgtggacag cgaggtatcg acctcctgac caatcaccag ttgcgcatta
    25321 tgtaaatccg ctggctgccc caccagatgc agcaaatcgc ccagttggat aatggtatct
    25381 ggcgaaggta cttttagggt ttcttctcgt ttcagacgcg agcagataat tttgtcgccg
    25441 ttgagaatcg gtacatcttt aatggctaaa tcatgcaggt tagggttctc aacgcgaata
    25501 ttgatagtcc tgatcagcgc gccgccattg gtgcgtgaag attcgtgctg ctgggcttct
    25561 gtctcgacat tgacgcggaa aataacccgc aacatccaca tggtgaacaa aatcccgcaa
    25621 atgccgaatg gatacgccat cgcgtaactc atccccatct gatcgaccat ttccattggt
    25681 gtacccaggt cgcgcaagat ctgctgccct gcccccagcg ctggcgtatt ggttaccgca
    25741 ccggagaaaa tccccagcac taccggcagt ggaatatcaa acagtttatg caggatggcg
    25801 gtaaccagac caccgatgat gacgatcaga acagcaaaca ggttgaggcg taatccggag
    25861 acgcgcaatg aggcaaagaa gcccggccct acctgaatcc cgatggtata aacgaacagg
    25921 atcaggccaa attcctgaat aacatgtagc atatcgctac ttaatgtcat ccccgcctga
    25981 gaaacaaaat ggccgacgat gatcccacca aacagcacgc cgccaatacc taatcctatg
    26041 ccgcgaaatt tgacgttgcc gataaacaaa ccgacgactg ccaccaaagc cagaatactg
    26101 accgttaatg ctatatcact catcgtccat ttctcttgag ataacatttt aattgctacg
    26161 gattatggct gaatcggtga agcagatttg ccttatagcg cacattatca cgggaaagaa
    26221 cgtgccgaaa tatcttaaac agtcgaaatt taacgcattg ttgaagaaga aagacaaaaa
    26281 aagccccacc agaatggcgg ggcaaagaga atagctagtt agctatttaa cgcgggacgt
    26341 tcgctgatag cgatacgctg cgctgcgatg ggttcaggct cattacgaat taaatcaata
    26401 tgcagtaaac cgtttacgaa ggttgcgcca gagacttcca tattttcagc cagcgtaaag
    26461 ctcaggctaa atggctgatt cataagccct tgatgcagcc attttttctc ttcttttggc
    26521 tgctccggcg tgccttttac gctcaggcgc gtaccttcca gttgaatctc taaatcttcc
    26581 tgacggaaac ccgccagcgc aagggtaatg cggtagtggt tatcgtcgct tttctcaatg
    26641 ttgtacggcg ggaagctctg actttcaccg gcgttttgca gtgcgttggc cagtttgtca
    26701 aaaccgatcc attgacgcat cagtggggat aaatcgaagt taggcatagt catttctcct
    26761 tctaagaagc gagtaagtac ctgcaaatcc gaagattcgc atatgctccc tgacggcgag
    26821 catggagatg tcaggccgcg caaggcggcc ttagggaatt agttgatttc gatacggcgc
    26881 ggttttttcg cttccggaat cacgcgttcg agatcgatat acagcaaacc atttaccagg
    26941 ttagcgccac gaacatgaat gttctcagct aactggaatt tgcgttcaaa gttgcgttca
    27001 gcgatgccct ggtacagata ggtgcgctct ttttgttcgt cggcgtgagc acctttcacc
    27061 accagcagat tatcctgggc ggtaatttcc agttcgctct cagcaaaacc agccacagcg
    27121 atagcaatgc ggtaatggtt ttcgtctacc agttcaacgt tatacggagg gtagccgcca
    27181 ttactctggc tctggttgtt ttctaagtgg ttaaacaaac ggtcaaatcc aatagcagaa
    27241 cggtaaagcg gggataaatc aaagttacgc ataatcaata gctcctgaaa tcagcgagaa
    27301 tgtaagacct tccacaatgg acaggtcagg tagccagaac acccatcagg cgcgtcctca
    27361 tcggctacga tgtaaaaatg ggtctggaaa tgaacttttc aagccttatc agcggacttt
    27421 tttttgcagt ttatggtcta ttgcatagac tgagggggca gcacgaaacg ttaaagtgcg
    27481 atagccgcca cagtgcgacg taatgatgat gttattttca gcaagggatc gctataactc
    27541 atcaggccaa cacccacgat caacagatga atttattatg ataagaaatg tgttgttagc
    27601 gttcatgata tgcagcggaa tgacattact cggaggatgc tccagcgtga tgtcccatac
    27661 cggggggaaa gaaggaacgt atcccggcac ccgcgccagc gccagcatga ttggcgacga
    27721 tgaaaccaac tggggcacta agtcgctggc gattctcgat atgccgttta ccgctgtgat
    27781 ggacacactt ttactgccgt gggatgtgtt ccgcaaagac agctcggtgc gctcacgcgt
    27841 tgaaaaaagc gaagccaacg cgcaagccac caatgcggtt attccaccgg ccagaatgcc
    27901 tgataactga tttagcgatc agtttcggtg atccacagct gaccgccttc catccacgcc
    27961 agccattgac catccggcga gaagaccacg gcgtccgcag aaggtggatt ggcgtggttt
    28021 tcggttaaat actcaacctc cccgctttcc gcatgggcac aagcaattcg attatccagc
    28081 acaaagccca accattctcc tgatggatgc cagttaaatg cagactgaat atccgttttg
    28141 ttatgggtta actggcgcgg ctcgccgccc tgtggcgaga taagccacag ttgcacaatg
    28201 ccgttatcat ctcgcattaa aaacgcgatt tgcgtaccct gcggattaca gcgcacccag
    28261 tggcgcggga cgttgactaa ccccggataa gcccgatggt gggtaaaggt taaacgtcgc
    28321 tgcacgacgc cacgcggtgg cgcgggcagg gttgtttccg ttccgcttaa cggcgcatca
    28381 cctgctgctt tccagccagc ttcatcttgc ggtaactcaa cgataaacag ctccggcact
    28441 ttctcgctct ttggcgaaag tgtgtcgcca ataaacgcca gcgcttgatt tcctacccat
    28501 ccttcttcat aagcacgatt gatttcatcg ctgccaggct gcggcgtggg cgttgtttta
    28561 ctcaccagtg cgcaccagtg gctaccgctg tattcacgcg gatgctgctt ttgtacgttg
    28621 accgggccaa acggtgcagc aaccccgacg tttcgcaaat ccagcgctgg gctgcgttca
    28681 tgcattacat ggtcgttata ggtaaagctc accatttcac cgttcgggct aaagacatgc
    28741 acatggctgc cgccgcgcag cgctcctggg gtgtacggtg cagtaatatc cattgcatcg
    28801 agattgctca ccttgccgcc ttcagcaatg actccgcgtc gatgatggaa atcgtaatgc
    28861 catgtttcat caggattttc agggccgtga ataaagacat atttatctga cttcggatga
    28921 acggtcacca cgccgacgtg tgcgccctgt gacgcgcgat agataacctc gacctcgccg
    28981 gtatggatat tcacacgctc aatggtctcg ccggtaaacg acgccccaga aggacgcacg
    29041 tcaaatacca gccactggct gtcgggtgtc caggtattgg tattggtgag caggtgatta
    29101 cggggagcaa aggttatctg tttcatggcg cggtcctgat acaaaacggt tcccgccatc
    29161 atacttcaca aggacttcac tttcagacaa tagtcttttg ccatcgtgat gaatgtgagt
    29221 aaaaagatgg aacatttcga cgtggcgatt attggcctcg gcccggcagg gtcggcgttg
    29281 gcgcgaaagt tggcaggcaa aatgcaggtg atcgcgctgg ataaaaagca ccagcatggt
    29341 actgaaggtt tcagcaagcc ttgcggcggt ctgctggcac cggacgcgca gcgatctttt
    29401 attcgcgatg gactgacgct tcctgtcgat gtgatcgcca acccgcagat tttcagcgtc
    29461 aaaactgtcg acgtcgccgc atcgctcacg cgtaactacc agcgaagcta tatcaatatt
    29521 aatcgccatg ctttcgactt gtggatgaaa tcgctgatcc ccgccagcgt tgaggtttac
    29581 cacgacagcc tgtgccggaa aatctggcgt gaggatgata aatggcatgt catttttcgt
    29641 gcagacggtt gggagcagca tattactgcc cgctatctgg tcggtgcaga tggtgccaac
    29701 tcgatggtgc ggcgatatct ctacccggac catcaaattc gtaaatatgt cgctatccag
    29761 cagtggttcg cagagaaaca tccggtgccg ttctactcct gcatctttga taatgcgata
    29821 actgactgtt actcatggag tatcagcaaa gacggttatt ttatctttgg cggtgcctat
    29881 ccgatgaaag acggtcagac gcgtttcatg acgctgaaag agaaaatgag cgcctttcag
    29941 ttccagtttg gtaaggcggt aaaaagcgaa aaatgcacgg tgctatttcc ctcacgctgg
    30001 caggattttg tctgcggtaa ggacaacgcc tttctgattg gtgaagcggc gggatttatc
    30061 agcgccagct cgctggaggg gataagctat gcgctggata gcgcagagat tctgcgttcg
    30121 gtgttactga agcagccaga gaagatcaac gcagcctact ggcacgccac ccgcaaacta
    30181 cgtttaaaac tcttcggcaa gatagtaaaa agccgatgcc tgaccgcacc ggctttaaga
    30241 aagtggatta tgcgcagtgg tatggcacat attccacagt tgaaggatta gccaacgcgc
    30301 ttcacatcgc ccaccagcag gatgtaagag agcgcgccaa tcaacgcgac ggcggagata
    30361 taaaccagtg ccggggcgac cccatagcct tgcaacagat attagtccac aaaaaaacac
    30421 ctcataagaa aaggtcttat atcgggcaga aatctgaaaa gggagatcag gtgctaaaca
    30481 ggagggaaag aagagtatgc tttcaacggc ttagctactc gtttaaagga ttaatcatga
    30541 agttgaattt taagggattt tttaaggctg ccggtttatt cccactggcg ctgatgcttt
    30601 cgggctgtat ctcgtatgcc ctggtttccc ataccgcaaa gggtacttca ggaaagtatc
    30661 aatcgcagtc agacaccatc acggggctat cgcaggcaaa agatagtaat ggaacaaaag
    30721 gctatgtttt tgtaggagaa tcgctggact accttatcac tgatggtgcc gatgacatcg
    30781 ttaagatgct caatgatcca gcacttaacc ggcacaatat tcaggttgct gatgacgcaa
    30841 gatttgtttt aaatgcgggg aaaaagaaat ttaccggcac aatatcgctt tactactact
    30901 ggaataacga agaagaaaag gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac
    30961 actgtaccag gtcactggaa aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg
    31021 attactcaaa ggtgatggcg ttctaccatc catttaaagt gcgattttat gaatactatt
    31081 cacccagagg cattccggat ggtgtttccg cagcattact gccagtgact gttacgctgg
    31141 acatcattac tgcaccgctg caatttctgg ttgtatatgc agtaaaccaa taatcagtaa
    31201 gcgggcaaac gcgtttatgc tgtttgcccg cccacagatt aattcagcac atacttttca
    31261 atggcaaacg ccacgccatc ttcaaggttg gatttggtga caaagttcgc cacttctttc
    31321 accgaaggaa tagcattatc cattgccaca ccgacgcctg catattcaat cattgcaatg
    31381 tcgttttcct gatcgccaat cgccatgatt tcttccggtt taatacctaa cacgtcggcc
    31441 agtgatttca cccccgtacc tttgttaacg cgtttatcga ggatttcgag gaagtacggc
    31501 gcacttttca gcacggtata tttctctttc acttcctgcg gaatacgcgc gatagcctgg
    31561 tcgaggatgg caggttcatc aatcatcatc actttcagga actgggtatt ggggtccatt
    31621 ttctccgctt cgcagaacac cagcggaata gtggcaacaa aggattcatg caccgtgtag
    31681 tagctgatat cacggttggc ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa
    31741 ccgacttcgc gagagagttt ttccaggaaa cggtagtcgt catagctgag agcggtttgc
    31801 gccacggtgc taccatcagc ggccttctgt accagcgcgc cgttataagt aatgcagtag
    31861 tcacccggct gttccatatg cagctctttc aggtaattgt gaacacctgc ataagggcga
    31921 cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg cggcaatcgc atttttaacg
    31981 gcaggtgaaa tggtgtgatc gggcagcaga agggtgccat ccatatcgat agcaatgagt
    32041 ttgatagcca tgagttcccc aggtagattg gttcctgacc catgctaacg cgattccgct
    32101 caaaaatcag tacaacaccc gagggaaaag ggggatgcaa cgcgggttgc atgctcccca
    32161 tttgcttagc ggaagagttt ccctttcagc agttccatgc ctgcggaaag cagatcgtta
    32221 ttggcttgtg gtgacacttc accttgcggt gagagcgcat caataatctt cggcaattgt
    32281 tctgccagta aactggaagc tgtactgtta tccacgccaa gtttttgccc gagatcggac
    32341 accgcatttg tgccgagcgc cgattccagt tgttcgccgc tcaccgattg attgccctgt
    32401 tgattactca gccaggttga gagaatggcc cctaagccgc cactttgcag tttttccagc
    32461 agcacctgaa tgccgccctg ctcctcaacc caacttaaaa tcgcctgata tttccccgca
    32521 tcgcctttca gaaaggcgcc gacaacttca tcaaaaagcc ccatgataat cacctgtaaa
    32581 gcgttacgtg ttgacccaaa aagtatagaa ctgcgggatg ataattgcgg attgcagaaa
    32641 taaaaagggc ggagatgatc tccgcccttt tcttatagct tcttgccgga tgcggcgtga
    32701 acgccttatc cggcctacaa aaacatgcaa attcaatata ttgcaggagt ctcgtaggcc
    32761 tgataagcgt agcgcatcag gcacgctcgc atggttaacg ccattaaata tcgatattcg
    32821 ccgctttcag ggcgttctct tcaataaacg cacggcgcgg ttcaacggcg tcgcccatca
    32881 gcgtggtgaa caactggtca gcggcaatcg catccttaac ggtaacgcgc agcatacgac
    32941 ggctttccgg gtccatggtg gtttcccaca gctgttccgg gttcatctcg cccagacctt
    33001 tataacgctg gatggagagg ccgcgacggg actctttcac cagccagtcc agcgcctgct
    33061 cgaagctggc taccggctga cgacgctcgc cacgttcgat aaacgcatct tcttccagca
    33121 agccacgcag tttctcaccc agcgtgcaga tacgacgata ttcgccaccg gtgataaact
    33181 cgtgatccag cggatagtca gtatccacac cgtgggtacg cacgcgaaca atcggctcga
    33241 acaggttttg ttcggcattg gtgtggacat caaacttcca ctggctgccg tgctgttctt
    33301 tgtcgttcag ttcgctgacc agcgcgttca cccaccgggt aacggtctgc tcatcagaga
    33361 ggtcggcttc cgtcagcgtc ggctgataga taagctcttt cagcattgct ttcggataac
    33421 gacgctccat gcggttgatc attttctgcg tcgcgttgta ctcagacacc agtttctcta
    33481 acgcttcgcc agccaatgcc ggtgcgctgg cgttggtgtg cagcgttgcg ccgtccagcg
    33541 cgatagagat ctggtactga tccatcgctt cgtcgtcttt aatgtactgt tcctgcttgc
    33601 ctttcttcac tttgtacagc ggcggctgag cgatgtagac gtgaccgcgt tcaacgattt
    33661 ccggcatctg acgatagaag aaggtcaaca gcagcgtacg aatgtgcgag ccgtcgacgt
    33721 ccgcatcggt catgatgatg atgctgtgat aacgcagttt gtccgggttg tactcgtcac
    33781 gaccgatacc acaaccaagc gcggtgataa gcgtcgccac ttcctgagaa gagagcatct
    33841 tatcgaagcg cgctttctcg acgttgagga ttttaccctt cagcggcaga atcgcctggt
    33901 tcttgcggtt gcgcccctgc ttcgcagagc cgcccgcgga gtccccttcc acaaggtaca
    33961 gttcggaaag cgccggatcg cgctcctggc agtctgccag tttgcccggc aggcccgcta
    34021 agtcgagcgc acctttacgg cgggtcattt cacgagcgcg acgcgcagct tcacgggcac
    34081 gggcagcatc gataattttg ccaaccacga ttttcgcgtc ggttgggttt tccagcaggt
    34141 attccgccag cagttcgttc atctgctgtt ctaccaccga tttcacctca gaagaaacca
    34201 gtttgtcttt ggtctgggag gagaatttcg ggtccggcac ttttacggaa acgaccgcaa
    34261 tcaggccttc acgcgcatcg tcaccggtgg cgctgacttt ggcttttttg ctgtagcctt
    34321 ctttgtccat gtaggcattc agggtacggg tcatcgccgc acggaagcct gccaggtgag
    34381 taccgccgtc acgctgcgga atgttgttgg taaagcagta gatgttttcc tggaagccat
    34441 cgttccactg cagcgccact tcaacgccaa taccgtcttt ttcggtggag aagtagaaga
    34501 tatttgggtg gatcggcgtt ttgttcttgt tcagatattc aacgaacgct ttgatgccgc
    34561 cttcatagtg gaagtggtct tctttgccgt cacgcttgtc gcgcagacga atggaaacgc
    34621 cggagttgag gaacgacaac tcacgcagac gtttcgccag aatttcatat tcgaactcgg
    34681 tcacattggt gaaggtttcg aggctgggcc agaaacgcac catggtgccg gttttttcag
    34741 tctcgccggt aaccgccagc ggagcctgcg gtacaccgtg ttcgtagatc tgacggtgaa
    34801 ttttaccctc gcgctggata accagctcca gtttttgcga cagggcgttt actaccgaaa
    34861 caccaacgcc gtgcagaccg ccggacactt tataggagtt atcgtcaaat ttaccgcctg
    34921 cgtgcagaac ggtcatgatc acttccgccg ccgatacgcc ctcttccggg tgaataccgg
    34981 tcggaatgcc gcgcccatca tcctgtacgg agacagagtt atcggcgtga atggtgacga
    35041 taatttcttt acagtgaccc gcgagcgctt cgtcgatagc gttatctacc acctcgaata
    35101 ccatgtggtg cagaccggtg ccgtcatccg tgtcgccgat atacataccc gggcgcttac
    35161 gcaccgcatc cagccctttc aggactttga tactggagga gtcataagaa ttcgacatca
    35221 acgtttctcg ctcatttata cttgggttaa tccgttattt tacccttttc cacggtaaac
    35281 atcttcgaat tttcgtccga catgtctata acgtgttcag cactgatcgc gctgacaaag
    35341 acctgtgatt gcgtcgcttt taagcggctg gcaagcagcc cgcgacgctc atcatcaagc
    35401 tcagaggcaa aatcatctat caggtagaga caccgccgcc cgctttcacg ggtgaggaac
    35461 tctccttgcg ccagacgtaa ggcgcacatc aacagcttaa gctgcccacg cgataaggta
    35521 tcttccaccg gcgcaccgtc ggcacgaatg cgtaagtccg ctttatgcgg gccgtgcgcg
    35581 gtgtaggtta gctggcgatc gcgttcaaaa ttacgttcca gcacctcagc atattctgtc
    35641 tctttctccc agccgcgctg gaaagagaaa gtcagagaaa actcagggag aaattgctta
    35701 caggtatcag ccatatcggc cgcgataccg gcgctatact ccgcgcgcca ggtgctgatt
    35761 tgctctgcca gcgggatcag ttctttatcc cacgggcgta gctgttcgta acgagtcacc
    35821 tggcgcagcg ccgcattgcg ctgcttgagc aatcgcttga gattgctcca ggcggtgaaa
    35881 aatccgggtt cgttgtgaaa gcatccccag tcgaggaatg ctcttctgta tttggggccg
    35941 ccgttgagta aagtaaaccc ttctggcgtt atcaactgca tcggcatcag gtgcgccagt
    36001 tccgcgactt tatgcccgtc cgtaccgtca atacggactt tgctgtcgcc ctgtttgtct
    36061 ttggttaaac caatcgccgt ctcgcgctct tcgccctgta atcgcccgtg gagaacaaac
    36121 gcctcctgct catggcgaat gacgcgacca atctgcaaac tgcgaaacgc ccgaccatgg
    36181 ccgagcgtat agatggcttc cagcacgctg gttttgccac tgccgttggc acctaccaga
    36241 aagttaaagc cgggagataa agcgagatcc gcggtttcaa tgttgcggaa atcgcggatc
    36301 aacaagcggg tgagggacat tacagtctca ttggcatgac aacataagcc gcgctctggc
    36361 tggctgcatc ttcaatctgc acgctggaaa ccgaatcggt cagcatcatg cggacgtttt
    36421 cgcatttcag cgcgttcagg acatccagca catagctgac gttgaagccg atttccatct
    36481 ccgcaccgct ataggtaacg tcgaggatct cttccgcttc ttcctgttcc gggttattgg
    36541 cggtgatttt cagctggttt tcgctgacat acagacgcac gccgcggaat ttctcgttag
    36601 agagaattgc cgcgcgagca aacgcctgct tgaggagatc gcagcctgct tccagatgtt
    36661 tgtccgggtt cttcggcaga acgcggcgat aatccgggaa gcgaccatcc accagtttgg
    36721 aggtgaagat aaagtcgcca acgtgggcgc gaatgttgtt gctgccaatc tgcacacgca
    36781 gcggattatc gccgccgtcg agcatacgca tcagttcaat cacgccttta cgcggcacga
    36841 tcaccgaatg gcttggcaaa gattgaccaa ttggcattga acagactgcc agacggtgac
    36901 catcggtcgc cacggtgcgc agttcttcac cttcggtttc aaacagcata ccgtttaagt
    36961 aatagcgaac gtcctgatgc gccatcgaaa actgggtcgc ttcaatcaga cgcttcatcg
    37021 tcgcctgcgg cagggtaaat tcgacttcgc tctgccagtc atcgaggttc gggaaatccg
    37081 ccgctggcag ggtagacagc gaaaaacggc tacgcccgga gcgcaccagc atccgctcac
    37141 cttccagttg cacggcaatt tccgcgcctt caggcagacc acggcagata tcaaagaatt
    37201 tgcgcgccgg aacggtcgtc gcaccaggtt cgtgcggctg aaccagcgca acgcgtgcca
    37261 ccatttccat ctcgagatca gtaccggtca gcgacaacgt accgtcagca acctgtaaca
    37321 gcagattacc gagaatcggt agcgtaggac gaccacctaa cggaccgctc acctgttgta
    37381 gcggttttaa taaatgctca cgttctacgg taaatttcat aggtttacga tgacaatgtt
    37441 ctgattaaat ttgaaaaatc ttctttgata tcgtggctct cttcacgcag ctgctcgatc
    37501 ttccggcagg catgaagcac cgtcgtgtgg tcacgaccac caaacgcatc gccaatctct
    37561 ggcagactgt ggttagtcag ctctttcgcc agcgccatcg ccatctgacg cggacgagcc
    37621 accgagcggg atcgacgctt ggaaaggaga tccgcgactt tgatcttgta gtactccgcc
    37681 accgtcttct gaatattgtc gatggtgacc agtttttcct gcaatgccag caagtcgcgc
    37741 agcgcctcac gcacgaagtc gatagtgatc gcccgtccgg taaagttggc gttagcaata
    37801 acgcggttca gcgcgccttc cagctcacgt acgttagatc gtagacgctt ggcgataaag
    37861 aacgccactt cgcctggcaa acgaatgtca ttttcgtcgg cctttttcat caggatcgcc
    37921 acgcgggttt ctaactctgg cggttcgatc gccacagtca gcccccagcc gaagcgggat
    37981 ttcaaacgat cctcaacgcc gttgatctct ttcggatagc gatccgaggt gagaatgatc
    38041 tgttgattac cttccagcag ggcgttgaag gtgtggaaaa actcttcctg agatcgttct
    38101 ttattagcaa aaaactgaat atcgtcgatc agcagcgcat ctacggaacg gtagtagcgt
    38161 ttaaactctt cgatcgcgtt gttttgcagg gctttaacca tgtcctgaac aaagcgctcg
    38221 gagtgcatat aaaccacttt ggcattcggc ttgcgcgcca taatgccgtt acccaccgca
    38281 tgcagcaggt gagttttacc cagacccgtg ccgccataaa ggaacaacgg gttataggca
    38341 ccgccagggt tatctgccac ctggcgagcc gccgcgcgcg ccagttggtt agatttacct
    38401 tcaacgaagt tatcaaacgt gtgtttgacg tttacgttag aacgataggt cggttctgcc
    38461 ggggccggga cgttatccca acctgagcgc gtagaaggcg cagcacgttg cggctgcgtt
    38521 tgcgccacct gtgcaggggc cgcgacgttg ctcgtcactg ccgcttgtgg cgtttgcgtc
    38581 accggtttgg tgccgacttc aaaacgcagc tgtggggcat ccgctccgca gaaactggtt
    38641 agcagtccat tgatattatt aaggtacttg tcccgtaccc aatcgaggac aaaacggttt
    38701 ggcgcgtaca gggccagcgt gttatcgctc agttccgcct gcaatgggcg tatccacata
    38761 ctgaattctg tggctggtaa ctcatcctgc aatcgggcaa gacactgctg ccaaagcgaa
    38821 agtgacacgg cggactccac tcgaacaaaa gtcgataatg actaaggctg aaacattcat
    38881 gattgttgac gtacgtcgaa aagaccctgc ttgagggtga cgcacgaacc gctgtctgcg
    38941 gttatatgcc cgatcaagat cctgcaaaac gatcgggacc gcggatcata gcctaaactg
    39001 cgcaagagat cttctgtttc tcacagattt tcccgattta tccacaggat gatccagaac
    39061 tcgctaagtg taaacgatcc tgccgcgagg cgggcacgat ttacgccgca tattggaaaa
    39121 tttaatgacc atagacaaaa attggcttaa tcgatctaat aaagatccag gacgatcctt
    39181 gcgctttacc catcagcccg tataatcctc cacccggcgc gccatgctgg ttttcactgg
    39241 tgtgaggtcg tacattttcc ctgcgaaaag gtgcggaaaa gcgcggtaaa taaggaaaga
    39301 gaattgactc cggagtgtac aattattaca atccggcctc tttaatcacc catggcttcg
    39361 gtgtccatcg tttcattttt cggcggatat ccaataaagc cattgaattt attcaagttt
    39421 aggtagaaat cgccatgaaa cgcacttttc aaccgtctgt actgaagcgc aaccgttctc
    39481 acggcttccg tgctcgtatg gctactaaaa atggtcgtca ggttctggca cgtcgtcgtg
    39541 ctaaaggccg cgctcgtctg accgtttcta agtaataaag ctaacccctg agtggttaag
    39601 ctcgcatttc ccagggagtt acgcttgtta actcccagtc aattcacatt cgtcttccag
    39661 cagccacaac gggctggcac gccgcaaatt accattctcg gccgcctgaa ttcgctgggg
    39721 catccccgta tcggtcttac agtcgccaag aaaaacgttc gacgcgccca tgaacgcaat
    39781 cggattaaac gtctgacgcg tgaaagcttc cgtctgcgcc aacatgaact cccggctatg
    39841 gatttcgtgg tggtggcgaa aaaaggggtt gccgacctcg ataaccgtgc tctctcggaa
    39901 gcgttggaaa aattatggcg ccgccactgt cgcctggctc gcgggtcctg atagccctca
    39961 ttcgggtcta tcaacgcctg attagtccgc tactcgggcc gcattgtcgt ttcactccaa
    40021 cctgttcaag ctacggaatt gaggcattgc gcaggtttgg agtgataaaa ggcagttggt
    40081 tgacggtgaa acgcgtatta aaatgccacc ctttacaccc tggtggtgac gatcccgtcc
    40141 cgcccggacc atttgatacc agagaacact aacgatggat tcgcaacgca atcttttagt
    40201 catcgctttg ctgttcgtgt ctttcatgat ctggcaagcc tgggagcagg ataaaaaccc
    40261 gcaacctcag gcccaacaga ccacgcagac aacgaccacc gcagcgggta gcgccgccga
    40321 ccagggcgta ccggccagtg gccaggggaa actgatctcg gttaagaccg acgtgcttga
    40381 tctgaccatc aacacccgtg gtggtgatgt tgagcaagct ctgctgcctg cttacccgaa
    40441 agagctgaac tctacccagc cgttccagct gctggaaact tcaccgcagt ttatttatca
    40501 ggcacagagc ggtctgaccg gtcgtgatgg cccggataac ccggctaacg gcccgcgtcc
    40561 gctgtataac gttgaaaaag acgcttatgt gctggctgaa ggtcaaaacg aactgcaggt
    40621 gccgatgacg tataccgacg cggcaggcaa cacgtttacc aaaacgtttg tcctgaaacg
    40681 gggtgattac gctgtcaacg tcaactacaa cgtgcagaac gctggcgaga aaccgctgga
    40741 aatctccacc tttggtcagt tgaagcaatc catcactctg ccaccgcatc tcgataccgg
    40801 aagcagcaac ttcgcactgc acaccttccg tggcgcggcg tactccacgc ctgacgagaa
    40861 gtacgagaaa tacaagttcg ataccattgc cgataacgaa aacctgaaca tctcttcgaa
    40921 aggtggttgg gtggcaatgc tgcaacagta tttcgcgacg gcgtggatcc cgcataacga
    40981 cggtaccaac aacttctata ctgctaatct gggtaacggc atcgccgcta tcggctataa
    41041 atctcagccg gtactggttc agcctggtca gactggcgcg atgaacagca ccctgtgggt
    41101 tggcccggaa atccaggaca aaatggcagc tgttgctccg cacctggatc tgaccgttga
    41161 ttacggttgg ttgtggttca tctctcagcc gctgttcaaa ctgctgaaat ggatccatag
    41221 ctttgtgggt aactggggct tctccattat catcatcacc tttatcgttc gtggcatcat
    41281 gtacccgctg accaaagcgc agtacacctc catggcgaag atgcgtatgc tgcagccgaa
    41341 gattcaggca atgcgtgagc gtctgggcga tgacaaacag cgtatcagcc aggaaatgat
    41401 ggcgctgtac aaagctgaga aggttaaccc gctgggcggc tgcttcccgc tgctgatcca
    41461 gatgccaatc ttcctggcgt tgtactacat gctgatgggt tccgttgaac tgcgtcaggc
    41521 accgtttgca ctgtggatcc acgacctgtc ggcacaggac ccgtactaca tcctgccgat
    41581 cctgatgggc gtaacgatgt tcttcattca gaagatgtcg ccgaccacag tgaccgaccc
    41641 gatgcagcag aagatcatga cctttatgcc ggtcatcttc accgtgttct tcctgtggtt
    41701 cccgtcaggt ctggtgctgt actatatcgt cagcaacctg gtaaccatta tccagcagca
    41761 gctgatttac cgtggtctgg aaaaacgtgg cctgcatagc cgcgagaaga aaaaatcctg
    41821 attcggtgag ttttcgctaa aataagggcg gtcagttgac cgcctttttt cttttcgtag
    41881 ggcggataag caccgcgcat ccgccacaca aagcaacagg aacatcatga gcgataatga
    41941 cactatcgta gcccaggcca cgcctccggg acgtggcggc gttggcatcc tgcgcatctc
    42001 cggcttcaaa gcccgtgaag ttgccgaaac cgtgctgggt aaactgccta agccgcgcta
    42061 cgccgattat cttccgttta aagacgccga cggcagcgtg ctcgatcagg ggattgcgct
    42121 atggttccct ggcccgaact cgttcaccgg cgaagatgtg ctggaactgc agggtcatgg
    42181 cggtccggtg atcctcgacc tgctgttaaa acgcattctg accattcccg gcctgcggat
    42241 tgctcgccct ggtgagtttt ccgaacgcgc gtttcttaac gataaactcg acttagccca
    42301 ggccgaggcg attgccgatc ttatcgatgc cagttcggaa caggcggccc gttcggcact
    42361 taactcgctg caaggcgcat tctccgcacg ggtcaaccat ctggtggaag ccctcaccca
    42421 cttgcgcatt tacgtcgaag cggcaattga tttccccgat gaagagatcg atttcctctc
    42481 cgacggaaaa attgaagccc agcttaatga cgtgattgcc gatctcgatg cagtgcgtgc
    42541 tgaagcacgt cagggtagtt tgttgcgcga agggatgaag gtggtgattg ccggacgtcc
    42601 taatgccggt aaatcgagcc tgttaaacgc gctggcgggc cgtgaagcgg caatcgtaac
    42661 cgatatcgcc ggaaccacgc gtgacgtgct gcgtgagcat atccatattg acggaatgcc
    42721 gctgcatatc atcgataccg ccgggctacg tgaagccagt gacgaagttg aacgtattgg
    42781 gatcgagcgc gcgtggcagg aaattgaaca ggccgaccgc gtgctgttta tggtcgatgg
    42841 caccacgaca gacgccgttg atccggcaga gatctggccg gaatttatcg cccgtctgcc
    42901 agcgaaactg ccgatcaccg tggtgcgcaa taaagccgat atcaccggcg aaacgctggg
    42961 aataagtgaa gtgaacggtc acgcgttaat tcgtctgtct gcaaggactg gcgaaggcgt
    43021 ggacgtgctg cgtaaccatc tcaaacagag catgggcttt gacaccaaca tggaaggcgg
    43081 cttcctggcg cgccgtcgcc acctacaggc gctggaacag gcagcggagc atttgcagca
    43141 aggtaaagcg caactgttag gtgcctgggc aggcgaacta ctggcggaag agttgcgcct
    43201 ggcgcagcag aacttaagcg aaatcaccgg ggaatttacc tctgacgacc tgctggggcg
    43261 gattttctcc agcttctgta ttggtaagta agtcgtctta attgatataa agggcactgt
    43321 tccgttctac tatcagaacg gaacagtgct tgttttttta tgagcatcct gcaccaaaac
    43381 tgcctttact actggctgga gcgactttca acatcttgca gtcctgtctt tacagagaac
    43441 gatattggtt taaccgatta agctcctctt gcggaaagtt aaattcgtct attgctttcc
    43501 agaatagttc tttaaataca ggattgccat ttgccttgct tattacatat ttgccttcat
    43561 tattcttcgc ttccagttgc atcatcacat ctttaagata attttcattt tcaccaacca
    43621 caactttgaa tattttcagc cagagtctca tatgtatatc atccgctttc tctaccgcaa
    43681 gagataatga attcccatca aagtcgcggg taaactgctc cataatttca tcgaaagtga
    43741 atccgtgttg caacgccagg cgaatcgccg cattcagata tacatcaatg acctgcttac
    43801 tcattgtctt atagctggca acatacaaag agcagacagc attatttctg ctcagcgaca
    43861 gtagttctct cactacatct ttatcatttt cataatggct ggctatcact tcagctgtct
    43921 ccatccaggc agcaacaatc tccgtatgcc ctttcactat cgcagaatat aagcctgatg
    43981 ccttcgtcca tttgtctgat gctcccgcca ctttttctct tgctgccatc agtttcaata
    44041 tatcttcttt atttaattca tttttaaaca ttttagcttt tatattggct agcaatatac
    44101 taacaacatt ataatcattg gttctaatag cgctatttaa accataagtt gagtctattt
    44161 catttactat actaagaagc atttcatttt tatttacctc aaattctttc tcaaagtcat
    44221 ctatcgccaa caaccatgct ttgataattt tattttgatt attccttaaa gc
//
LOCUS       DAZFFG010000024        41619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFFG010000024 DAZFFG010000000
VERSION     DAZFFG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.024"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..707)
                     /locus_tag="ACHJ7J_001699"
     CDS             complement(<1..707)
                     /locus_tag="ACHJ7J_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4999948.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEVDIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFI"
     gene            complement(723..1520)
                     /gene="rfaP"
                     /locus_tag="ACHJ7J_001700"
     CDS             complement(723..1520)
                     /gene="rfaP"
                     /locus_tag="ACHJ7J_001700"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO4999949.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(1513..2637)
                     /gene="waaG"
                     /locus_tag="ACHJ7J_001701"
     CDS             complement(1513..2637)
                     /gene="waaG"
                     /locus_tag="ACHJ7J_001701"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4999950.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2634..3692)
                     /gene="rfaQ"
                     /locus_tag="ACHJ7J_001702"
     CDS             complement(2634..3692)
                     /gene="rfaQ"
                     /locus_tag="ACHJ7J_001702"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO4999951.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTSLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            4105..5382
                     /gene="waaA"
                     /locus_tag="ACHJ7J_001703"
     CDS             4105..5382
                     /gene="waaA"
                     /locus_tag="ACHJ7J_001703"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO4999952.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSSSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            5390..5869
                     /gene="coaD"
                     /locus_tag="ACHJ7J_001704"
     CDS             5390..5869
                     /gene="coaD"
                     /locus_tag="ACHJ7J_001704"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO4999953.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFNHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(5908..6717)
                     /gene="mutM"
                     /locus_tag="ACHJ7J_001705"
     CDS             complement(5908..6717)
                     /gene="mutM"
                     /locus_tag="ACHJ7J_001705"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO4999954.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(6815..6982)
                     /gene="rpmG"
                     /locus_tag="ACHJ7J_001706"
     CDS             complement(6815..6982)
                     /gene="rpmG"
                     /locus_tag="ACHJ7J_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO4999955.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(7003..7239)
                     /gene="rpmB"
                     /locus_tag="ACHJ7J_001707"
     CDS             complement(7003..7239)
                     /gene="rpmB"
                     /locus_tag="ACHJ7J_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO4999956.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(7456..8124)
                     /gene="radC"
                     /locus_tag="ACHJ7J_001708"
     CDS             complement(7456..8124)
                     /gene="radC"
                     /locus_tag="ACHJ7J_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4999957.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            8296..9516
                     /gene="coaBC"
                     /locus_tag="ACHJ7J_001709"
     CDS             8296..9516
                     /gene="coaBC"
                     /locus_tag="ACHJ7J_001709"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO4999958.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVIPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            9494..9952
                     /gene="dut"
                     /locus_tag="ACHJ7J_001710"
     CDS             9494..9952
                     /gene="dut"
                     /locus_tag="ACHJ7J_001710"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO4999959.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            10059..10655
                     /gene="slmA"
                     /locus_tag="ACHJ7J_001711"
     CDS             10059..10655
                     /gene="slmA"
                     /locus_tag="ACHJ7J_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO4999960.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10692..11333)
                     /gene="pyrE"
                     /locus_tag="ACHJ7J_001712"
     CDS             complement(10692..11333)
                     /gene="pyrE"
                     /locus_tag="ACHJ7J_001712"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO4999961.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(11399..12115)
                     /gene="rph"
                     /locus_tag="ACHJ7J_001713"
     CDS             complement(11399..12115)
                     /gene="rph"
                     /locus_tag="ACHJ7J_001713"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO4999962.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            12242..13105
                     /gene="yicC"
                     /locus_tag="ACHJ7J_001714"
     CDS             12242..13105
                     /gene="yicC"
                     /locus_tag="ACHJ7J_001714"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO4999963.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            13327..14150
                     /gene="dinD"
                     /locus_tag="ACHJ7J_001715"
                     /pseudo
     CDS             13327..14150
                     /gene="dinD"
                     /locus_tag="ACHJ7J_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            14442..15059
                     /gene="yicG"
                     /locus_tag="ACHJ7J_001716"
     CDS             14442..15059
                     /gene="yicG"
                     /locus_tag="ACHJ7J_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO4999964.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFCARLLALRLKLGLPVFYYSHEGH"
     gene            complement(15056..16738)
                     /gene="ligB"
                     /locus_tag="ACHJ7J_001717"
     CDS             complement(15056..16738)
                     /gene="ligB"
                     /locus_tag="ACHJ7J_001717"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO4999965.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQQVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            16996..17619
                     /gene="gmk"
                     /locus_tag="ACHJ7J_001718"
     CDS             16996..17619
                     /gene="gmk"
                     /locus_tag="ACHJ7J_001718"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO4999966.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17674..17949
                     /gene="rpoZ"
                     /locus_tag="ACHJ7J_001719"
     CDS             17674..17949
                     /gene="rpoZ"
                     /locus_tag="ACHJ7J_001719"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO4999967.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            17968..20076
                     /gene="spoT"
                     /locus_tag="ACHJ7J_001720"
     CDS             17968..20076
                     /gene="spoT"
                     /locus_tag="ACHJ7J_001720"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO4999968.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            20083..20772
                     /gene="trmH"
                     /locus_tag="ACHJ7J_001721"
     CDS             20083..20772
                     /gene="trmH"
                     /locus_tag="ACHJ7J_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO4999969.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITEEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            20778..22859
                     /gene="recG"
                     /locus_tag="ACHJ7J_001722"
     CDS             20778..22859
                     /gene="recG"
                     /locus_tag="ACHJ7J_001722"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO4999970.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALNVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(23035..24240)
                     /gene="gltS"
                     /locus_tag="ACHJ7J_001723"
     CDS             complement(23035..24240)
                     /gene="gltS"
                     /locus_tag="ACHJ7J_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO4999971.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            24520..25911
                     /gene="xanP"
                     /locus_tag="ACHJ7J_001724"
     CDS             24520..25911
                     /gene="xanP"
                     /locus_tag="ACHJ7J_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO4999972.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            26032..27741
                     /gene="yicH"
                     /locus_tag="ACHJ7J_001725"
     CDS             26032..27741
                     /gene="yicH"
                     /locus_tag="ACHJ7J_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4999973.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTGQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNTSEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSKVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAMNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(27794..30112)
                     /gene="yicI"
                     /locus_tag="ACHJ7J_001726"
     CDS             complement(27794..30112)
                     /gene="yicI"
                     /locus_tag="ACHJ7J_001726"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO4999974.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEEG
                     EVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHETVCEVPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(30122..31504)
                     /gene="yicJ"
                     /locus_tag="ACHJ7J_001727"
     CDS             complement(30122..31504)
                     /gene="yicJ"
                     /locus_tag="ACHJ7J_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO4999975.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIVAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            31797..31891
                     /locus_tag="ACHJ7J_001728"
     tRNA            31797..31891
                     /locus_tag="ACHJ7J_001728"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31831..31833,aa:Sec,seq:tca)
     gene            31970..32131
                     /gene="rhuM"
                     /locus_tag="ACHJ7J_001729"
                     /pseudo
     CDS             31970..32131
                     /gene="rhuM"
                     /locus_tag="ACHJ7J_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            32529..33713
                     /gene="setC"
                     /locus_tag="ACHJ7J_001730"
     CDS             32529..33713
                     /gene="setC"
                     /locus_tag="ACHJ7J_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO4999976.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNVPVVTQPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            33824..34747
                     /gene="yicL"
                     /locus_tag="ACHJ7J_001731"
     CDS             33824..34747
                     /gene="yicL"
                     /locus_tag="ACHJ7J_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO4999977.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(34751..35569)
                     /gene="nlpA"
                     /locus_tag="ACHJ7J_001732"
     CDS             complement(34751..35569)
                     /gene="nlpA"
                     /locus_tag="ACHJ7J_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO4999978.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            35780..36073
                     /gene="yicS"
                     /locus_tag="ACHJ7J_001733"
     CDS             35780..36073
                     /gene="yicS"
                     /locus_tag="ACHJ7J_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO4999979.1"
                     /translation="MKPTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            36375..36872
                     /locus_tag="ACHJ7J_001734"
     CDS             36375..36872
                     /locus_tag="ACHJ7J_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HFO4999980.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNIKMATELFNTVVTYSQNEKVKEMATAYLELLTANTEE
                     EVQTE"
     gene            36882..37064
                     /locus_tag="ACHJ7J_001735"
     CDS             36882..37064
                     /locus_tag="ACHJ7J_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999981.1"
                     /translation="MKINGKNASFTPEELARKYHEEATIDLKNNHVDKINEKYNNKHI
                     TYFPVDAILDGNKHLP"
     gene            37120..38901
                     /locus_tag="ACHJ7J_001736"
     CDS             37120..38901
                     /locus_tag="ACHJ7J_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III cell invasion protein SipB"
                     /protein_id="HFO4999982.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAMQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     TTRLHNNLDSTLLRHEMAHNKFRELSDAYASSLDSAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFAEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CESIKNQSEVMKEINHVRENKLNETARSYTTWTKLLNIINEVVTVVTFAVSAALIVIG
                     IVAAVPSAGASFAGALALIGGIAGAVVLGVDIICQITLGTTATGWILGKLTEGLSGII
                     SKYDPTLLAICALLDALGVQQTTIDLVQDIYSSAAASIVMITLMIGATLICSVAISEL
                     VKALSKTAAEEITKEITDSIKSTVESMIKSVSKKIMSALNFLGDKIKASSKFIMIFAK
                     ITILLERIAAFFCTIITSSMNQYAASKSADIEILQQDISNLSKTREQMLTVMQRIDKT
                     VEQEVAQMVRVLQHRTEALKFASHSIV"
     gene            38914..39087
                     /locus_tag="ACHJ7J_001737"
     CDS             38914..39087
                     /locus_tag="ACHJ7J_001737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999983.1"
                     /translation="MTNNISNINNTAHNKNLDKTTFDEQPGYETRVSQAKKYCKVFRQ
                     MILKLTQILNLLV"
     gene            39357..40043
                     /locus_tag="ACHJ7J_001738"
     CDS             39357..40043
                     /locus_tag="ACHJ7J_001738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999984.1"
                     /translation="MSIVFEQLVKLQSDETKNASWANLTGAIVGSAINATGAVVMAGA
                     EIAKPAGKIDDIAENQEQNTDKPDDQPRLKSKEDLANAKTRLAEIETQQKELSTNQNI
                     EDNDKQEQSSKLQKEKESITNKIKDKEKINSHNRRYSISSSISHTNNLFQSIANLSQY
                     GGTVAAEHTRAEKDLAEQAKNVMHSVMSSYLDQINRDVQGRDQALNLLQQIIQTIIDT
                     DSAIVQGIRS"
     gene            40087..41436
                     /locus_tag="ACHJ7J_001739"
     CDS             40087..41436
                     /locus_tag="ACHJ7J_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HFO4999985.1"
                     /translation="MINKDNNLNIDILRQQNANIPLKEASSQSEEPHQLENSKSKGNL
                     HNSASKDNNIGAMLKKDVEKLSTLVSERSPRNEDWKCQHDIQLNFVKNMLRNFPTKNL
                     SEKGSALLHESYLLIKNVLQLTENVNTIDGKLKDPAKILTLHNSVRKTLANIADNTTG
                     SSAMASTKGTLNHAIASLDSSAPEMRSSATSSTLTASSSSEFKSDKYICSDIADLMSV
                     LGSDYLEIYANSVEIMSAYWQNFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHF
                     YYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTP
                     SPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAE
                     YQAWLAGYNSQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
BASE COUNT        10927 a        10557 c         9867 g        10268 t
ORIGIN      
        1 tcaatgaatt taactttccc taacaggata agattaagga tatcttgatc catataggtt
       61 aaacgggaaa cgatggcttt atcagcaagc atcgacatcg ctttagcaga aacggactcc
      121 tgcgcccacg ctagtgtatt aattaacagg acacctgaat taaagtaacc cttttcaagt
      181 tcattacact gcagactttg accccgtaac gaccaccagt tagcatcgcg ttcagtaaca
      241 acagcagcaa cgttattggc aagatccatt gttatcagcg gctttaagtt tccctgacag
      301 gcgatatcag catccaggta aaggatctta tcttgttgat caataaagta atctgcaatt
      361 acaaaacgga aatacatggc aatagaccaa tttttggtcg tcggtaatgc cttaagccgt
      421 tcacaattta ctagatggat ctggatacag gtacgatagc ttttcgccaa ttgggctaaa
      481 cgctggatat cgacttcagg gatatcatca ataaaaacgt ggaaaacaaa actcacgtcg
      541 ttgttatgta acaaaactga cgtgattgaa acaccacaac caaaaagaaa gtttttatca
      601 atgccataag caacatggaa tgatgatgcc actgacgccg ctggcctttc atcgaagatg
      661 attgtcttat tgatcatctc ttgtggatta aaatagtggg cactcatatt tctctccgga
      721 aattacaacg attttcgaat cgttctttcc ctgatttttg tggcttttgc ttctgcttgc
      781 gacagcagtc cttgttcctg cttgagaatg tctttaagcg gggcggcaaa atacactttc
      841 ataaaccgcc agatatcccg ctgagtcagg ccgatattca tcgaagaaaa ataaagccca
      901 ataagatctt tatcgcgcca acgacgtgga acgcgcgtgc gaagctgcgc ccggtgcagg
      961 tcaattaccg aaatttttaa ctcctcttcc ttaccggaaa aaggcaagtg cagcaggaaa
     1021 tgacagatat aacagtcacg gtggttaatg cccgcagcat gcatatcgcg caccatcgtc
     1081 gcgacacgct taataagcat acgctttacg cgaacatctg gagggttagt cgcccagtca
     1141 gcacagtaat cttccagact tatggttggt gtcagatctt cggtaataat aaatgaagtt
     1201 ctggtcagcg gattcatgcc tttttcgcca aatgccaccc catacatagt atcaacgccg
     1261 acatcccgca gtcgatgaat cgcattccat tcgcggtctg cgcctaatac tggcatccgc
     1321 aatgagagta aatttttgat tatctctttc agggtcgtgc cgcgatgcca tttgagaaaa
     1381 tagcttttgc ccgccatttc aaagcgcaga gtacggcgag tttccagttc acgaaatacc
     1441 tcaccctgca aggttttaac ttcctcaaaa ggatctttac cgcgccataa cgtggcaaac
     1501 ggctctttaa gttcaaccat ccagaccacc cgttatgata tccgccgctt tctctggcag
     1561 actgtataaa tcttgtgtat cagcataatg tcgcgcattt tccgcccagg cctggcgcaa
     1621 tgaagattgc gttaacgctt tgcgtaaaat ctcattcaat gtttcctggc ggaatggctc
     1681 aacaatagcc tcgccgcaat tggcctcgac aatataatgc gcatagccac aaacggctgt
     1741 tgttagtacc ggtaatcctg cagtaatcgc ttccagcagc acaattcccg ccgcttcctg
     1801 gtaggcagga tgcagtaata aatccgccgc cgccattaat tccgagacat cgttgcgccc
     1861 cgagaagaag tgaacattac tgcgcacgcc gcgtttttct gccagcgcct caaattttcg
     1921 cggtttatcc tgcccaacaa catatagcaa tgtgttgtgg cgcagcgaat ccggtaacga
     1981 agcaagtgct tcaatggaac gatcgacacc tttacgcgtg aagtctgaac cgacctgcaa
     2041 caataaatat tgttgttcgg ttatcccatt cttcttacgg aagatttcac ggctattggc
     2101 tggctgctgg ctatatttac gatcaggata aatccccggt ggcagaatat gaaaacgctc
     2161 cgcttcagtc tgataatgtt tctggaaatc ggcgatttgc ttatctgtca gcatcagcag
     2221 ctgtgtcggc ttgccctgtt cgaaggttgc ccgctcaaag gcggcataat ggcgataacg
     2281 tgacgtcagg cgatagaaaa agcctttttc ctgcgctact ttctcggcat aacaaacatc
     2341 agcggcataa taaacgtcca gccccggcat tttgttgaat ccaacgactt tatcgacggg
     2401 atgttcgcgt aaatgttttt gcacccaggc aaaatactcc gcattgcgcc cgtgattggt
     2461 atgcgattta accggcactt tgatcagttc aaatacatca gggcattcgc cttcccacga
     2521 ctgggtataa acccgaacat gatgacctcg ggcggcgact gtctgagcaa tacgcataaa
     2581 atcgcgctgc aaaccgccaa agggaaaata tttatataaa caaaaagcaa cgatcataac
     2641 gacgtacctg tcgtggagga gggcagtaat ttatcgacag cagcaatgac atctgccgcc
     2701 ggaataaccg aaagatacat ctcatttcgg tcacgctgat cgcgcgttgg catttcccgg
     2761 taatctcccg cccagaattg aatcatgtta tttgaccagg gacgccagaa aatatggtct
     2821 gtcgcaccaa acagcgatat cagcggcgta ttaactgcag cggcaatatg cgccggtgcg
     2881 gaatcaacgc caataaacag ctgcgcatga tcgattaacg caccaagttc cgggaaggtc
     2941 acctttccag ccagcgccgt tactggtggc gtctggcatc cctgcgcaat ttcattgacg
     3001 caggccagat cgtctttatc tgggccggac gtcagaacga cttcataacc acgagcatgt
     3061 aaggcatcaa tcacagcgga aaacttggcg ttgtcccagc atttgaagat ttgccgcgcc
     3121 gtaggttgga taaccacata attttgtcca acaccagcgt gatcaagttc gcgacgcata
     3181 cgtttccagc ttgcaggcgg gtaactcatg gttgtctgct tcaccaacga atcaagtccc
     3241 aatgatgtca gcacggataa gttactttcc accacatttc caccctgcaa cggcaccaaa
     3301 tgggtgaaac ttttacgcca aaaagcagac tgccgatgat gataatcctg ggaaattttc
     3361 acacgggcat ttaataagcg aaccagtata gcaaccatcc attgatcggt aagattgacg
     3421 ataaggtcat acttattggc acgtaatacc ttgatgagat gaaaaaagtt ggcaattttt
     3481 tctgaggctt ttgctttttt attttttatg ccgtagagcg cgttaatctc tggattttca
     3541 gacaggatcg ggatggtgtc ctgataaagc agcacatcga tttttgcgtc agggtaattt
     3601 tttttcagcg aactaatgac gggagtagtt aataacatat ccccatgaaa acgcatctta
     3661 atgagcaaaa ttcttcgaaa tggcttatcc acaagtaact ctttttgttg agattatccg
     3721 attaaggtga agcagaaaaa gcacgctacc gccccaggct caacagctac ctgaatactg
     3781 ataccgcact gatattgacg cgctagtgta tcacttcttt cgcggcaatc cgacccgaat
     3841 gatttttaag cacattgttg attttcaata aagaaatcaa tcaaataaaa tcattagcaa
     3901 ccatattctt tcactgttgc tctgcgattc cttcctgcac gtacagcaga aatcaatgaa
     3961 tcgcataaaa atacaaaaaa agtatagatg ttcagcaaga attggcgggt aaattgctat
     4021 ttaagtcaaa aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc
     4081 acatccataa gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt
     4141 attcagccgc tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga
     4201 aaacgctggg gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg
     4261 ctgcactccg tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt
     4321 catcgttatc ctgatttacc gattaccgta acaaccatga cgccaaccgg ttcggagcgc
     4381 gtacaatcgg ctttcgggaa ggatgttcag cacgtttatc tgccgtatga tctgcccgat
     4441 gcactcaacc gtttcctgaa taaagtcgac cctaaactgg tgttgattat ggaaaccgaa
     4501 ctatggccta acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac
     4561 gcgcgactct ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgtcgc
     4621 ttgctgcgtc gtattacgct gattgccgcc caaaatgaag aagatggtgc acgttttgtg
     4681 gcgctgggcg caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct
     4741 gtaacgccgc agttggctgc taaagccgtg acgctgcgcc gccagtgggc accacaccgc
     4801 ccggtatgga ttgccaccag cactcacgaa ggcgaagaga gtgtggtgat cgccgcacat
     4861 caggcattgt tacagcaatt cccgaattta ctgctcatcc tggtaccccg tcatccggaa
     4921 cgcttcccgg atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct
     4981 tcaggggaag tcccttcctc cagcacgcag gttgtggttg gcgatacgat gggcgagctg
     5041 atgttactgt acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt
     5101 gggcataatc cgctggaagc tgccgcacac gcaattccgg tattgatggg gccgcatact
     5161 tttaacttta aagacatttg cgcgcggctg gagcaggcaa gtgggctgat taccgttacc
     5221 gatgccacta cgcttgcaaa agaggtttcc tctttactca ccgacgccga ttaccgtagt
     5281 ttctatggcc gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg
     5341 cttcaactgc tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg
     5401 ggcgatttat ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg
     5461 cgccacgcag atgttcaatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc
     5521 gatgtttacc ctggaagagc gtgttgagct ggcacagcag gcaaccgcgc atctggggaa
     5581 cgtggaagtg gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac
     5641 ggtgctgatt cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca
     5701 tatgaatcgc cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg
     5761 gtcgtttatc tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca
     5821 tttcctgccg gagaatgtcc atcaggcgct gatggcgaag ttagcgtagc gtttatgccg
     5881 gatggtatgc catccggcgc gcatgaatta cttctggcac tgccgacaat aaaacgttgc
     5941 ccgctgcgca tgtttagtcg ccacaatcgg cgtaccgcac acccgacagg gctcgccttt
     6001 tcgcccgtaa acctgcaatt cctgagcgaa atagcccggt ttaccatcac tttgcagaaa
     6061 atcttttagc gttgtaccgc cctgctcaat cgaacgcagc aacaccgctt taatcacccg
     6121 agccagcaat tcgcactccg ccagcgacag tgatgacgcc agccgatccg gatggatccc
     6181 cgccgcaaac agagattcgc tggcatagat attccctacg ccgaccacca gcttgttatc
     6241 catcagccac ggtttgatcg ccgttttttt cttcgcgcac ttctgatgca ggtactcacc
     6301 gttgaaatcg tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag
     6361 ctctttggtc cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc
     6421 gttgctcatc accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat
     6481 gcgcaggctg ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag
     6541 cagatattta gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc
     6601 ttctgaaacc ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc
     6661 aacgagatgc ggttctatgc cgcggcgact ggtttcaact tcgggtaatt caggcatagc
     6721 atctccagga atgaacagat gcagtcaata tgggggcaag caaataacaa aaaaccccgc
     6781 cggagcgagg ttttttgtta catcaaagcg gaaattattt gattttcgct tctttgtaga
     6841 tcacgtgctg gcgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac
     6901 gtttgttctt cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt
     6961 tctcacgaat acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg
     7021 ggcacgcagt tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt
     7081 agcagatacg cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg
     7141 caggttcggc aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt
     7201 caccggacgc ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa
     7261 ttagctcgag cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc
     7321 agttctatgt gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg
     7381 gcgtagtata cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg
     7441 atcgcgcata gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc
     7501 aatcacgata tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc
     7561 agtaatgagt ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag
     7621 gatcagcgcc gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc
     7681 aacatggttt agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc
     7741 gagaaagatc accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc
     7801 cctcgtcatc tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg
     7861 ccgcgccagt tcagcaatcc cctttaactg ggcaaatttc gccacgccaa ttccatgaac
     7921 accactaaat tgttcatatt cagaggttaa caagccataa agagagccga aattctccag
     7981 catctctttt gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc
     8041 cagcagctcg acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat
     8101 caacagctgt gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag
     8161 ccgttccgtt aaatcgacgg ccagttttca atcctggaaa gcgcctcgca aagtgattct
     8221 caggtgaagg cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca
     8281 acaggaaaaa tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt
     8341 attgctgcct ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc
     8401 cgcgtagcca tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt
     8461 tctggttatc ccgtttccga cagtctgctg gacccggcag ccgaagccgc tatgggccat
     8521 attgagctgg gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt
     8581 gcccgtgttg ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct
     8641 gcgcctgtag ccgtgatccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag
     8701 cataatttag aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt
     8761 caggcttgtg gtgatatcgg tcctgggcga atgctcgatc cgttaaccat tgtggatatg
     8821 gcggtggcgc atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc
     8881 ggcccgacgc gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag
     8941 atgggttttg ctatcgccgc cgccgctgcc cgtcgtggcg cgaacgtcac gctggtatca
     9001 ggtccggttt cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gactgcgctg
     9061 gaaatggaag ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggctgcgcc
     9121 gccgtggcgg attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg
     9181 cagggtgatg aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc
     9241 gcactaaaag accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa
     9301 gaatacgccc ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt
     9361 tcccagccaa ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac
     9421 ggagataaag tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag
     9481 atcgtgaccc gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttgggaa
     9541 ggaatttccg ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg
     9601 tctcgacgac gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc
     9661 gattcatatt gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca
     9721 taagcacggt atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca
     9781 gttgatgatt tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg
     9841 catcgcccag atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt
     9901 cgacgccacc gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg
     9961 catccgaata acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc
    10021 cgcctggcaa gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga
    10081 aaaggaaccg tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg
    10141 gaagccaacg tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac
    10201 tgtatcgcca cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag
    10261 atagcctgat tactcgcatc aatctgattc tgaaagatga gaaagacacc acagcgcgcc
    10321 tgcgtctgat agtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca
    10381 tcctcactgg tcatgcgctg atgtttgaac aggatcgcct gcaagggcgc atcaaccagc
    10441 tgttcgagcg tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg
    10501 gtgaaggtta caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag
    10561 gtatgctgtc acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg
    10621 cccgctggcc gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc
    10681 ggcgagtttc tttaaacgcc gaactcttcg cgataggcct taaccgccgc cagatgttcc
    10741 gccatttctg gcttctcttc caggtaagca atcaagtctt tcagggtaat gatagagatc
    10801 accttgcagt tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccccgt
    10861 tcctgacgat caagcgaaat caatacgcca gcaagcgtcg cgccattagc ctgaataatc
    10921 tccatcgact cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt
    10981 ccttgtaacg cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ctctttgcgg
    11041 ttaaagcagt acggcaggtc aaggtcatga tgctccgcca gcgccacggc ggtagtggtg
    11101 gcaatcggga tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc
    11161 accaacgctt cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc
    11221 ccggcgttga agaaataggg gcttttgcgc ccggacttca gcgtaaactc gccaaacttt
    11281 aacacctgct tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg
    11341 ctcctcatct tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc
    11401 agtttgccag cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg
    11461 ccaacaaggt gagtaactct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt
    11521 caatgatgcg cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt
    11581 caacgtattc cagatcgcaa attgcttcgc cgttaacaat accgacagaa accgcggcta
    11641 ccatcccttt cattggattg gttttcagct tgccgttttc caccagcttc tgtaacgcat
    11701 ctgccagcgc cacgcaggca cccgtaatcg aagcggtacg cgtgccacca tccgcctgaa
    11761 gcacgtcgca gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcac
    11821 gaagagcacg ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg
    11881 ccgcttcacg agcgttacgg gtgtgggtag aacgcggcag cataccgtac tctgcggtga
    11941 tccagccctg gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac
    12001 acaacacttt ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt
    12061 tacgagtcag ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga
    12121 aatctccggc ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta
    12181 tcctgagaag gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc
    12241 tatgatccgc agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc
    12301 aacctgggaa atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga
    12361 gcagttccgt agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacacgcgg
    12421 taaagtggaa tgtaccctgc gctatgagcc agatgttagc gcgcaaggtg agctgatcct
    12481 caacgaaaaa ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgcagagtga
    12541 cgaaggggaa atcaacccag ttgatattct ccgttggccg ggcgtgatgg cggcccagga
    12601 gcaagatctc gacgcgattg ccgctgaaat tctcgcggcg ttggatggca ccctggatga
    12661 ctttatcatc gctcgcgaaa ccgaaggtca ggcgctgaaa gcgttgattg agcagcgtct
    12721 ggaaggcgtc accgccgaag tggtcaaagt ccgctcccat atgccggaaa tcctgcaatg
    12781 gcagcgtgaa cgcttggtcg cgaagctgga agatgctcag gtgcagttgg aaaacaaccg
    12841 tctggagcag gagttggttc tgctggcaca acgaattgac gttgccgaag aactggaccg
    12901 cctcgaagcg catgttaaag agacgtacaa cattctgaag aaaaaagaag ccgttggtcg
    12961 tcgtctggac tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc
    13021 tatcaatgcc gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg
    13081 cgagcagatt cagaatattg agtaactcgt aacactaatt cttacttttc agccttcaca
    13141 aatgccgccc actcaaacag agcggcattt ttcttccccg caactttcaa ttctgttttg
    13201 cgtgcctgct ccagattttg cgatgtcttt ttgcccagca cactgagaac gtgagatact
    13261 cacaactgta tataaataca gttacagatt tactttcttt gcaatcgata tcacatggag
    13321 tgggcaatga acgaacatca tcaacctttt gaagaaatac ggcattatgg cactgaaggg
    13381 caagaatttt ggtcagcgag agaattggca ccgttactgg attatcggga ctggcgcaat
    13441 tttcaaaagg tactggcccg cgcgactcaa gcgtgcgaag ccagcaatca agctgcatct
    13501 gaccatttcg ttgaaaccac caaaatggct gtattaggtt caggggctca acgagaactt
    13561 gaagatgttc atctctcccg ctatgcctgt tacctggtag tacaaaacgg cgaccctgcg
    13621 aaaccggtta ttgcggcagg gcaaacttat tttgctatcc agacccgacg gcaggagctg
    13681 gctgacgatg aagctttcag gcaacttcgt gaagacgaaa agcgtctttt tctgagaaat
    13741 gaattgaaag aacataacaa acaattagtt gaggcagcgc agcaagctgg ggtagcaaca
    13801 gctatcgatt tcgccatatt tcagaatcat ggttaccggg gctgtatggt ggattagatc
    13861 agaaagctat tcatcagcgg aaggggctga aaaagagtca gaagatcctg gatcatatgg
    13921 gttcaacaga actggcggct aatctctttc gagctaccca aacagaagaa aaacttaagc
    13981 gggatggcgt gaattcaaaa cagcaagcta acaccacaca ctttgacgta ggtcgcaagg
    14041 tgaggcaaac cattcaggaa cttggcggaa ccatgcctga ggagttgtcg accccgcagg
    14101 tcagcattaa gcagttggaa aacagtgtaa aaattacaga gaaaaaatag cagaaactaa
    14161 aaactctttt tgttgattga gacacccgat gcgtaagcca aagtccaggt gcggtgaata
    14221 caatagctaa acattgcacc tttcttttcc acccctcagt tttaacctat tttttcttat
    14281 gcattttctc agacaagaag tcagaagaat gcatctccgc tacagaaaat agcgatttca
    14341 cataactaca agttatcaat tccccctcac cttaaaaaat ctcaatcgtg acaatgcgca
    14401 caaatcgcta ccctgccaga cagattttta gggagagaac catgctgtta cacattttgt
    14461 atttggttgg cattactgcc gaagccatga cgggggctct ggcggccggg cgacgccgca
    14521 tggatacatt tggcgtaatt attattgcga ccgccaccgc aattggcgga gggtcagtgc
    14581 gcgatattct gctgggccac tatccgctcg gctgggtcaa acacccggaa tatgtgatta
    14641 tcgtcgctac cgccgcagta ctcaccacta tcgttgctcc tgtaatgcct tacctgcgca
    14701 aagtgtttct ggtgctcgat gcgctcggac tggtagtctt ttctatcatc ggggcacagg
    14761 ttgcactgga tatggggcac ggcccaatta ttgccgttgt cgcagcggta accaccggcg
    14821 tgtttggcgg cgttttgcgc gatatgttct gtaaacgcat accactggta ttccagaaag
    14881 agttgtatgc cggtgtctca tttgcctccg ccgtgctgta catcgcgctg caacactatg
    14941 tttctaacca tgatgtggtg attatctcca ccctggtatt cggcttttgt gctcgtttac
    15001 tggcgctacg ccttaagctg ggattaccgg tcttttacta cagccacgaa ggtcactaag
    15061 gttcaaaacc tgtgatctgc tgggcagcca gccaactgcc cagcttcttg atttgcgcat
    15121 tttccttcca ttcaataacc tgtctggcgc gccccgatcc agtccccggc agctgctgcc
    15181 agaactgttc cgtgctaaat aaaagttgcg accaggaccg ttcatcactg gcattaagcg
    15241 ccgcccgggt tagcggtatt cccattgcca tcacccagcg agtaaaaggc tgcttacgag
    15301 ccagattaaa ctgatgccat aactgcgcac ttttactttt cgcgatcccc ggcgtgttct
    15361 gtaattgctc tggcgttaat aaaagccagg aaaagatatg ctcaaaccga tgagtctgat
    15421 gcagcgcgcg ccaaccggcc tcaccaatgc catccagccc cagaacctgt tttgacccca
    15481 gccagactaa gcgtgaaatg aactgttcct gacaaacatc agaagcaaag tagcaggtca
    15541 acgagttaaa gcggttttct ggtggtgtcg gttttgtacg ttctgcaccg cgccagacca
    15601 catcatcaat gcgaggaata ccctgaccgg caaggctgac gaggatctga tcaccaggcg
    15661 caatatccca ctcctgccag cgcctgacgg aaccaatatt cacctgctgg acttttttat
    15721 catccagcat gacaggtgca agtgacgcaa ccaccgatat tttaccgctc ttacccaccg
    15781 caaactgaat cgccttcact tcggcaacct gagctacagg ttgatatttc caggccacca
    15841 gccactccgc ctggcccggt agccaatgac gggattcggg ttccttagcc cctcgcacaa
    15901 ccacgccatc ggtgacgaag ggtaatttcg ctttccacca ctcattgcgt acgcgcgcaa
    15961 cctcatcagc atttttaacc gcacgggtat acctctgcgt tagagtaaaa cctgcggtag
    16021 ccagctcctt taaacgatcg gtcattaact gcggtccatc cggccatgcc cagacaaaaa
    16081 cagccagtga gtttagcgtg tcgctattcc cctggcgcat catcaggcca gcaacttttg
    16141 cgcgggcatt tattcccccc atttgttgtt ggatatgccc ctcgcgctgg agaaatattt
    16201 ccccctgaag cgtactgttg gctaaaggcc cgctaacggt ttgcggcaca gcggaaatta
    16261 agctaacttt ctgcgtccag tcctcgcctt tcaggccatt accgcgacta attgctttgt
    16321 tcagtttccc gtcccgataa accagggtta ccgcaacgcc atcaactttt ggctgcaccc
    16381 aaagatcgct acgttctcgc atccacagac ttaatgcgtt tttatccacc attttgcgta
    16441 cacccgtatg ggcaaccgga tgcataaccg caccatttaa aggcggcatc atcacgtcgc
    16501 ggggctcgtt cccaaagcag cgttgccact gcgtaagacg ggcgcttaac tgatcgtaaa
    16561 caccgtcttc cacctcactt tttccttcct tccagtagtc atcgtcccac tgttttattt
    16621 gctgttgcag gcgggaaatt tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg
    16681 cccacgccga tgattgccag cacaagatac ttattaatat cgccatccat actttcatct
    16741 tcacctccgc tgtagtcagg ctggcagata tacaacgtgg tgaaggcaaa gccgagtggc
    16801 aaaaacggag tctgcgagga cgcttcctga gaatcgtctt tattgcagta aatcacaggc
    16861 aaatgcggaa acagctacgc aaaacgcaac aactttgcgc aaaaagtgtg agcaagggct
    16921 acgtcacatg gccgcgccgt gtataataag ctcgtatgta ggctttattt cgctaatcac
    16981 atacgaaaga tactcatggc tcaaggcacg ctttatattg tttctgcccc cagtggcgcg
    17041 ggtaaatcca gcctgattca ggctttatta aaaacccaac cgttgtatga cacccaggtt
    17101 tctgtttcac acaccacacg ccaaccgcgt cctggtgaag tccacggtga acattatttc
    17161 tttgttaatc atgatgaatt taaagaaatg attagcagag atgcgttcct cgaacacgca
    17221 gaagtttttg gtaattacta tggcacttcg cgtgaggcca ttgagcaagt actggcgacc
    17281 ggtgtcgatg tttttctcga tatcgactgg cagggtgcgc agcaaattcg ccagaagatg
    17341 ccgcacgcgc ggagtatctt tattttaccg ccgtccaaaa ttgaactgga ccgccgtcta
    17401 cgcggtcgcg gtcaggacag cgaagaggtc attgcaaagc gtatggcgca agctgttgca
    17461 gaaatgagcc attacgccga atatgattat ctgattgtga atgatgactt cgataccgcg
    17521 ttgaccgatt tgaagaccat tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag
    17581 cgtcatgacg ctttaatcag caaattgttg gcagactgaa cctgatttca gtatcatgcc
    17641 cagtcatttc ttcacctgtg gagcttttta agtatggcac gcgtaactgt tcaggacgct
    17701 gtagagaaaa ttggtaaccg ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag
    17761 atgcaggtag gcggaaagga tccgctggta ccggaagaaa acgataaaac cactgtaatc
    17821 gcgctgcgcg aaatcgaaga aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc
    17881 caggaacagc aagagcagga agccgctgaa ttacaagccg ttaccgctat tgctgaaggt
    17941 cgtcgttaat cacaaagcgg gtcgcccttg tatctgtttg aaagcctgaa tcaactgatt
    18001 caaacctacc tgccggaaga ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt
    18061 gatgctcacg aggggcaaac acgttcaagc ggtgaaccct atatcacgca cccggtagcg
    18121 gttgcctgca ttctggccga gatgaaactc gactatgaaa cgctaatggc ggcgctgctg
    18181 catgacgtga ttgaagatac tcccgccacc taccaggaca tggaacagct ttttggtaaa
    18241 agcgtcgccg agctggtaga gggggtgtcg aaacttgata aactcaagtt ccgcgataag
    18301 aaagaggcgc aggccgaaaa ctttcgcaag atgattatgg cgatggtgca ggatatccgc
    18361 gtcatcctca tcaaacttgc cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc
    18421 ccggacaaac gtcgccgcat cgcccgtgaa actctcgaaa tttatagccc gctggcgcac
    18481 cgtttaggta tccaccacat taaaaccgaa ctcgaagagc tgggttttga ggcgctgtat
    18541 cccaatcgtt accgcgtaat taaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag
    18601 atgatccaga agattctttc tgaaatcgaa gggcgtttgc aggaagcggg aataccgtgc
    18661 cgcgtcagtg gtcgcgagaa gcatctttat tcgatttact gcaaaatggt gctcaaagag
    18721 cagcgttttc actcaatcat ggacatctac gctttccgcg tgatcgtcaa tgattccgac
    18781 acctgttatc gcgtgctcgg ccagatgcat agcctgtata agccgcgtcc gggtcgcgtg
    18841 aaagattaca tcgccattcc aaaagcgaac ggctatcagt ctttgcacac ctcgatgatt
    18901 ggcccgcacg gcgtgccggt tgaggtccaa atccgtaccg aagatatgga tcagatggcg
    18961 gagatgggtg ttgccgcgca ctgggcttat aaagagcacg gcgaaaccag cacgactgca
    19021 caaatccgcg cccagcgctg gatgcaaagc ctgctggagc tgcaacaaag cgccggtagc
    19081 tcgtttgaat ttatcgagag cgttaaatcc gatctcttcc cggatgagat ttacgttttc
    19141 acaccggaag ggcgcattgt cgaactgcct gccggtgcaa cgcccgtcga cttcgcttat
    19201 gcagtgcata ccgatatcgg tcatgcctgc gtgggcgcac gcgtcgaccg ccagccttac
    19261 ccgctgtcgc agccgcttac tagcggtcaa accgttgaaa tcattaccgc accgggcgct
    19321 cgcccgaatg ccgcgtggct gaactttgtc gttagctcga aagcgcgcgc caaaattcgt
    19381 cagttgctga aaaacctcaa gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac
    19441 catgctttgg gtggtagccg taagctgaat gaaatcccgc aggaaaatat tcagcgcgag
    19501 ctggatcgta tgaagctggc aacgcttgac gatctgctgg cagaaatcgg acttggtaac
    19561 gcaatgagcg tggtggtcgc gaaaaatctg caacatgggg acgcctccat tccaccggca
    19621 acccaaagtc acggacatct gcccattaaa ggtgccgatg gcgtgctgat cacctttgcg
    19681 aaatgctgcc gccctattcc tggcgacccg attatcgccc acgtcagccc cggtaaaggt
    19741 ctggtgatcc accatgaatc ctgccgtaac atccgtggct accagaaaga gccagagaag
    19801 tttatggctg tggaatggga taaagagacg gcgcaggagt tcatcaccga aatcaaggtg
    19861 gagatgttca atcatcaggg cgcgctggca aacctgacgg cggcaattaa caccacgact
    19921 tcgaatattc aaagtttgaa tacggaagag aaagatggtc gcgtctacag cgcctttatt
    19981 cgtctgaccg ctcgtgaccg tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg
    20041 ccagacgtga ttaaagtcac ccgaaaccga aattaatgtt ttatgaaccc aacacgttat
    20101 gcacgcatct gcgaaatgct cgccaggcgg cagcctgatc tgaccgtctg catggagcag
    20161 gtccacaaac ctcataacgt ttctgcgatt attcgtaccg cagatgccgt tggcgtacat
    20221 gaagttcacg ccgtctggcc tggtagccgc atgcgtacca tggcctctgc ggcggcgggt
    20281 agcaacagtt gggtgcaggt gaaaacgcac cgcaccattg gcgatgccgt cgctcatctc
    20341 aaaggccagg gcatgcaaat tctggcaacc catctttctg ataacgctgt cgattttcgc
    20401 gaaattgatt acactcgccc gacctgcatt ttgatgggtc aggagaaaac gggcatcact
    20461 gaggaagcgt tggccctggc agatcaggac atcatcattc cgatgatcgg catggtgcag
    20521 tcgctgaatg tttccgttgc ctcagccctc attctttacg aagcccagcg tcagcggcaa
    20581 aatgcaggca tgtacctgcg tgaaaacagc atgttgccgg aagcagagca acaacgcctg
    20641 ttgtttgaag gcggctatcc ggtgctggcg aaagtcgcaa aacgcaaagg cctgccttac
    20701 ccccacgtca atcagcaagg cgagatcgaa gctgatgccg actggtgggc taccatgcag
    20761 gctgcagggt aagtgtcatg aaaggtcgcc tgttagatgc tgtcccgctc agttccctaa
    20821 cgggcgttgg cgcagcgctt agtaacaagc tggcgaaaat caacctgcag actgtgcagg
    20881 atctactctt acaccttccc ctgcgctacg aagatcgcac ccatctctac cccatcggag
    20941 aactactacc gggcgtttat gccacggtgg aaggcgaagt gctgaactgc aatatctcct
    21001 tcggcggtcg gcggatgatg acctgccaga tcagcgacgg ttccggcatt ctcaccatgc
    21061 gctttttcaa cttcaacgcg gcaatgaaaa atagcctggc gacgggccgc cgcgtgttgg
    21121 cctatggaga agcaaagcgc ggtaaatatg gtgcggagat gatccacccg gaataccgcg
    21181 tgcagggcga tctcagtacg ccagagttgc aggaaacgct cacgccggtt tatccaacaa
    21241 ccgaaggcgt aaagcaggcc acgctgcgta aattaaccga ccaggcgctg gatctgctcg
    21301 acacctgcgc cattgaagaa ctcctgccgc cggaactgtc acaaggaatg atgacgctac
    21361 cggaagcgtt gcgcactttg caccgcccgc cgccgacgct gcaacttagc gatctggaaa
    21421 ccgggcagca tccggcgcaa cgtcgtctga ttctggaaga actgctggcg cataacctca
    21481 gcatgttagc cttacgcgcc ggagcgcaac gtttccatgc ccagccgctg agcgccaatg
    21541 acacgctgaa aaataaactt cttgccgcct taccgttcaa gccaacgggc gctcaggctc
    21601 gcgtggtggc ggagatcgag cgcgatatgg cgctgaatgt gccgatgatg cgcctggtac
    21661 agggcgatgt aggttccggt aaaacgctgg tcgctgccct cgccgcgctg cgtgcgattg
    21721 cccacggcaa acaggtggca ttgatggcac caaccgaatt actcgccgaa cagcacgcca
    21781 ataacttccg caactggttt gcaccgctcg gtatcgaagt gggctggctc gccggtaagc
    21841 agaaaggtaa agcgcgactg tctcagcagg aagccatcgc cagcggtcag gtgcagatga
    21901 ttgttggtac gcacgccatc ttccaggaac aggtgcagtt taacggtctg gcgctggtga
    21961 ttatcgacga acagcatcgt tttggcgtgc atcagcgtct ggcattgtgg gagaaaggcc
    22021 agcagcaggg cttccatccg catcagttga tcatgaccgc cacgccgatc ccccgcacgc
    22081 tggcaatgac tgcgtatgcc gatctcgata cctcggtcat tgatgaactg ccgccaggcc
    22141 gcacgccagt gactacggtc gctattcctg atactcgccg taccgacatc attgaccgtg
    22201 tgcgccacgc ctgcatgact gaaggtcgtc aggcgtactg ggtttgtacg ttgattgaag
    22261 agtcagagtt actggaagcg caggcggcgg aagctacctg ggaagagttg aaactggcgc
    22321 taccagagtt gaacgtcggc ctggtacacg ggcggatgaa accagccgag aaacaggcgg
    22381 tgatggcgtc gtttaaacaa ggtgaactac acctgctggt tgccactacc gttattgaag
    22441 tcggtgttga tgtgcctaac gccagtctga tgattatcga aaacccggag cgtctgggtc
    22501 tggcgcagtt gcaccagcta cgtgggcgtg taggtcgtgg cgcggtggct tctcactgcg
    22561 tgctgctcta caaaacgccg ctttctaaaa cggcgcaaat tcgcctgcaa gtgctgcgcg
    22621 acagcaacga cgggtttgtg attgcacaaa aagatctgga gattcgcggc cctggcgaat
    22681 tgttaggcac tcgtcagacg ggtaatgctg aatttaaagt ggcggattta ctgcgcgatc
    22741 aggcgatgat cccggaagtt cagcgcctgg cacgccatat tcacgaacgt tacccacaac
    22801 aggcaaaagc cctgatagaa cgctggatgc cggagacgga acgttactcg aatgcgtaaa
    22861 aggcggcagt gagaagaccg ccatttcagg ttaccctacc ttcctgccgg atgcgcttcg
    22921 tcctacaaaa acttataaat tcaataaatt gtgaatcaat gcgcagcctt gataagcgaa
    22981 gcgtatcagg catttgtctt tgttgcggcg gcacttctac cgccgcttca tcggttaacc
    23041 cgcaaaaatc ggcaacatca aatacagctt aatcaccagc gcgttaacga tatcaataaa
    23101 gaacgcaccg accatcggca ctaccaggaa ggccatgtgc gacgggccaa aacgttcagt
    23161 gatcgcctgc atgttggcga ttgccgttgg cgttgcaccg aggccaaaac cacagtgacc
    23221 cgcagccagc actgccgcat cgtagttttt gcccatcatg cgccaggtaa cgaagatggc
    23281 atacaacgcc atgaagatgg tctgtaccac cagaatcgcc aacatcggca gcgccagcga
    23341 agccagctcc cacagtttca gccccatcag cgccatcgcc aggaacaacg acaggcttac
    23401 gttacccagc acggataccg cacgctcaaa gacgcggtaa aagcccatca ttgacagacc
    23461 gttgctcaga atcactccaa caaacagtac acagacgaag gtcggcagtt caaaagcagt
    23521 gccagccaaa agttgcgcaa caattttccc caccgtcagg cagatagcaa tcagcgcgat
    23581 agtttcaatc agcaccagcg aggtgatcat gcggcccaca tccggctttt caaacgccgt
    23641 cgggacttcc tggtcatccg gaataccgtt cggcgtggtg gagtgtttca ccagatagcg
    23701 cgccaccgga ccgccaatca agccgcccag caccaaaccg aacgttgcac aggccatcgc
    23761 cacttccgtc gcattggtga agccataacg ttcaatgaac aatttactcc atgcagctcc
    23821 cgtaccgtga ccgccggaaa gcgtaataga accggccaac agccccatca gcggatcaag
    23881 ccctaacagg ctagccatac caatgccaat ggcattttgc atcaccaaca gaccaacaac
    23941 cacaatcagg aaaatgccaa ccacacgccc accggcacgc aaactggcaa tgttggcgtt
    24001 caggccaatg gtggcgaaga aagccagcat taacggatcg cgcagggaca tatcaaagtt
    24061 gacttcccag cccatgcttt ttttcagtac cagtagcgcc agcgccacca acaaaccacc
    24121 cgcaacaggt tccggtatgg tgtatttctt caaaaaggag acggaatgga ccaacttacg
    24181 cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct aaagtatcga gatgaaacat
    24241 agttactcct ttgtatccgc atcactcttt catactcgtc atacttcatc gcgcagtggc
    24301 aacttcatga aatatgacgg gtatcgagtt atgctttcct gggtcaaatt ctcagctgtt
    24361 aatgaacaaa tggcatggat tttaagcaga aaaataccaa aagttatata aaaaagttca
    24421 tttatctcat ttaaatattt agaagaggac gtagcaaacg tttgcttttc tctttcggtc
    24481 agctaaaatg cccgctttgc taccacggga ttgttttcga tgtctgtttc caccctcgag
    24541 tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg aactgattta ccgtcttgaa
    24601 gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc agcatttgct ggcgatgttc
    24661 gttgcggtga tcacgccagc gctattaatc tgccaggcgc tgggtttacc ggcacaagac
    24721 acgcaacaca ttattagtat gtcgctgttt gcctccggtg tggcatcgat tattcaaatt
    24781 aaggcctggg gtccggttgg ctccgggctg ttgtctattc agggcaccag cttcaacttt
    24841 gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg gtggtgctga tgttcctacc
    24901 atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt gcaccgagat ggtgatctcc
    24961 cgtgttctgc atctggcgcg ccgcattatt acgccgctgg tttctggcgt tgtggtgatg
    25021 attatcggcc tgtcgctaat tcaggtgggg ctgacctcaa ttggcggcgg ttacgcagcc
    25081 atgagcgata acaccttcgg cgcaccgaaa aatctgctgc tggcaggcgt ggtcttagcc
    25141 ttaattatcc tgcttaaccg tcaacgtaac ccttacttac gcgtggcctc actggtgatt
    25201 gcgatggcgg ccggatatgc gctggcgtgg tttatgggca tgttgccaga aagcaacgaa
    25261 ccgatgacgc aagaactgat tatggtgcca acgccgctct attacggtct tggcattgaa
    25321 tggagtctgc tgctgccgct gatgctggtc tttatgatca cttcgctgga aaccattggc
    25381 gatatcacgg cgacctctga cgtttccgaa cagccagtgt ccggtccgct gtacatgaaa
    25441 cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt ttgtttcggc ggtgtttaat
    25501 accttcccga actcctgctt cgggcagaac aacggagtga tccagttgac cggtgttgcc
    25561 agccgctatg tcggttttgt cgtcgcgctg atgttgatcg tgctgggtct gttcccggca
    25621 gtgagcggtt ttgtacaaca cattccagaa ccggttctgg gcggcgcaac gcttgtaatg
    25681 tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc gtgagccgct gaaccgtcgg
    25741 gcgattctga ttatcgccct gtcgctggcg gttggtctgg gcgtgtctca gcagccgctg
    25801 attttgcagt ttgcccctga atggctgaaa aacctgctct cctccgggat cgccgcgggc
    25861 ggtattactg ccatcgtgct gaatctgatt ttcccaccag aaaaacagta attcactggc
    25921 ggcggtaaga taattaccgc cgcagtctca cctctttcac cattcccgcc ttgaggattg
    25981 cgcgtaaatc gtgcataact cccttatgtg catttcacgg gatggaagac catgaaattt
    26041 attgggaagc tgcttctcta cattctcatc gctctgttag tggtgatcgc tggcctctat
    26101 tttcttctgc aaacccgctg gggagcagaa catatcagcg catgggtttc cgagaatagc
    26161 gactatcatc tggccttcgg ggcgatggat caccgttttt ccgcgccatc tcatatcgtg
    26221 ctggagaacg tcacatttgg tcgtgatggt cagcccgcga ccctggtggc aaaaagtgtc
    26281 gacattgcgc taagcagtcg gcaactgacc gaaccacgcc atgtcgatac catcctgctg
    26341 gaaaacggga cgctgaatct caccggccag accgcgccgc taccgttcaa agccgatcgt
    26401 ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg aatggaaact gagcgcgcag
    26461 cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg gtaaagtgct gggtacgaag
    26521 gcgcagattc agtttagtgc cggatcgctt tcgctcaatg atgttcctgc caccaatgta
    26581 ctgattgaag gcagtattga taacgatcgc gttacgctga ctaacctggg tgccgacatc
    26641 gcccgcggga cattaaccgg aaacgcgcag cgtaacgccg acggcagctg gcaagtggaa
    26701 aatctgcgca tggcggatat ccgtctacaa agcgaaaaat cgctaaccga cttctttgcg
    26761 ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg aagtgatcga tgctcgtctg
    26821 caaggtccgg actgggcggt gaccgacctc gatctcagct tgcgcaacat gaccttcagt
    26881 aaagatgact ggcagacaca ggaaggcaaa ctgtcgatga acaccagcga gttcatttat
    26941 ggttcgctgc atttatttga cccgattata aacgcggaat tttccccgca gggcgtagcg
    27001 ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca gaacgtcagg gaactggctg
    27061 cgtgacggga aaacgttgat ccttgatgat gcggcaattg ccgggctgga atataccttg
    27121 ccgaaaaact ggcaacagtt gtggatggaa acgacacccg gttggttaaa cagcctgcaa
    27181 ctgaagagat ttagcgccag ccgcaatctg atcattgata tcgaccctga cttcccttgg
    27241 cagcttaccg cgctcgatgg ttacggtgct aacctgacgc tggttaccga tcataaatgg
    27301 ggcgtctgga gtggttcggc gaatctgaat gccgccgccg cgacgtttaa tcgtgttgat
    27361 gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca aggtgaatat cagcgaactg
    27421 agtgcattta ctgaaaaagg cattctggaa gccactgcca gtgtttcaca aacgccacaa
    27481 cgtcagaccc atatcagcct gaatggacgc ggtgtgccgg tgaatatttt gcaacagtgg
    27541 ggatggcctg aattaccgtt gactggcgac ggcaatattc agcttaccgc cagtggcgat
    27601 attcaggcca atgtcccgtt gaaacctacg gttagcgggc aattgcatgc catgaacgcc
    27661 gcaaagcagc aagtgactca aaccatgaat gcgggcgtag tttccagtgg tgaagttacg
    27721 tcggcagagc tggtgcagta aacatcaatc tcgccgggta cgactcagac gtacccggca
    27781 ttccatcaat agattacaac gtaattgtca gcgcattccc ttgaggcgtc accaccagcc
    27841 cctgctcact ttcagcctgc gaaccgcctt gcagaccatt tactttcaca acattgcgca
    27901 agcacagcgt ccagttcttc gcctcgcccg caccagtcac agtaatcgtg ttgccagtac
    27961 gtgttgcttt tagagtaaag atcaccgaac cgtcagcagc gggcacttca cagacggttt
    28021 catgcccgtc ttgcagatta aagaggtgga atgccgtgcc ttcgtgccac gcgtaatcgg
    28081 gacgttgatc gttgttgccc agcgccagta gggtgttatc acgcacataa acgggcagac
    28141 tcaggaagcc gtgctgctgt ttatgccagc gactaccgtc gagctcatcg ttgtgccaca
    28201 ggtgtgtcca gcgaccttcc ggcaagtaga actgcacctc gccctcctca gtgaacaccg
    28261 gagcaaccat cacgttgtcg cctagcatgt attgacggtc aaggtaatca caagccggat
    28321 cgtccgggaa ctccatcatc atggcccgca tcatcggcgt accccgcgcg ttcgcacgcg
    28381 cagcttcacg atacagatac ggcatcatgc ggcatttcag ttgcgtgaag aagcgcacca
    28441 catcacagga ctcatcatcg taggcccacg gcacacgata agatttgctg ccatgtaaac
    28501 gactatggct ggagagcaaa ccaaacgcgc accagcgttt gtaaacgtgc gccggcgcgg
    28561 tattctcaaa gccgccgata tcgtggctcc agaagccaaa acctgaaagg ccaatagaca
    28621 aaccaccgcg caggctttcc gccattgatt cgtagttagc gtaacagtcg ccaccccagt
    28681 gtaccgggaa tttctgcgca ccgacggagg ccgagcgggc aaacaagaca gcttcttcct
    28741 caccaacggt gtccttgagc acgttccaca ccagttcgtt gtagatgtac gcataatggt
    28801 tatgcatttt ctgcggatcg gaaccgtcaa accactgaac atcggttggg atacgttcgc
    28861 caaagtcggt cttaaagcaa tcaacgccca tcgcgaccag acctttcagt ttgtcggcgt
    28921 accatttgca ggcatccgga ttggtaaagt cataaatcgc cagacctggc tgccatttat
    28981 cccactgcca cagcgaaccg tccgggcgtt tgagtaaata acctttctct tgtaactctt
    29041 taaagacggg ggatttttgc ccaatatagg ggttaatcca gacgcagatt ttcagccctt
    29101 tcgctttcag gcggcggatc atcccttccg ggtccgggaa agtcagcggg tcccactcaa
    29161 aatcgcacca ctggaaggct ttcatccaga aacagtcaaa gtggaaaaca tgcagcggca
    29221 gattgcgttc cgccatacca tcgataaagc tgtttaccgt cgcttcgtcg tagttggtgg
    29281 taaatgaagt ggttagccac aggccgaagg accatgcggg cggcagcgcc ggacgaccag
    29341 taaagcgggt ataacgatca agtaccgctt tcggcgtcgg accgtcgata acaaagtatt
    29401 cgagatattc gctctcaacg ctgaactgca ctttggagac tttctccgat cccacttcaa
    29461 aagagacaca ctgggggtga ttgaccagca ccccatagcc acggttagtc atatagaacg
    29521 ggatattttt atacgcctgt tcagtacttg tgccgccgtc ccggttccag gtttctaccg
    29581 tctgaccatt gcgcaccagg gcagtaaagc gctctcccag accataaact gtttcgccaa
    29641 cgccaagatc aagccgctca aacatataat ttcgttgatt attcgtgtcc tgcacgtagc
    29701 cattattttt cacctgacta ccggtaatac gttcaccgtt gcgcagaaaa tcgagtgacc
    29761 agaactcacc tttgctgaca cgcgcgctta agttgccact tttaaactca gcataacgtt
    29821 ctgtgttttc gattgtgacc ttcacatcct gcaaaatatt gagcggataa tgaggaccgt
    29881 tattcagcgc cccctgaaaa tgctcaatcc gaacaccgac aataccttcc tgtggggaga
    29941 aaaagcgcag cgtaaataaa ggcgtatcaa gctgccaggt acgttcacgc acatcacggg
    30001 gggcagcata gaccaccatt tcattaccct gctgttcaac ctcgaacacc tgaagcgggt
    30061 gaatcaaatt gaggccaggt tgaatcaacc agtttccatc gctaattttc attgccgttc
    30121 cttagttctg cacttcttga gaggtgaatt gttgctggca acggcgtttg ccctgggcca
    30181 gctgttccat aacggttttc agattgtgcg tcgtgagtga gtagtagcgt ttagcgacaa
    30241 tcgcgctcag caaataacag atcgccggaa caatcgtgaa tagcgcaata atgatgctaa
    30301 tcgtggcgct gttctgcgct ttttctgccg catcatatcc gccataagcc agcatccagc
    30361 cgataagagc gccgccgaag gccaacccca gtttgagcac aaacaacgtg ccagcaaaac
    30421 tgatcccggt caggcgttta ccattgcacc actcgccgta gtcgacggta tcggacatca
    30481 ttacccactg gataggtgtc accagttgat gcaacacacc aatcacgaag atgaagacaa
    30541 acatagtgat gctggcctgc atgggaacaa agaacatcgc gaggctaatc actgccagca
    30601 gggcgttcgt ccaccagaaa atagtgactt tacatttcca gtcggtcagc ggttttgcca
    30661 gtgcggaacc aatcaggtta cccacgcaat aagtggtgag aaaagcgaca aacacttccg
    30721 gcgtacccaa aatccatgtg acgtaataca tcatcgctcc accgcgtacg cacacagcca
    30781 ggatattgaa aatggttagt aaaccgacaa tccgccactg gtcgttttgc cagatatcac
    30841 gtaaatcttc ccgcatagac gtcgttgtag gtggtgcttc aacgcgttct ttagtggtga
    30901 agaaacagaa tgccagcatc atgaatgcca ccacggaaag gaccgcgata ccgccctgga
    30961 aaccgagtgg tttattatca ccaccaatta aattaaccaa tggcatcatc agaaccgtag
    31021 aaagcatgcc tcccgcggtc gccagcacaa aacgccagga ttgcagcgag atacgctgcg
    31081 tcgggtcatt ggtgattaca ccacccaatg cgcaataagg gatattaacg acggtataaa
    31141 gtaaggtaag tagggtgtaa gtaattgctg cataaatcat ttttccgttc atgctgagat
    31201 ctggcgtgct ataggccagt acacagacga tcccgaatgg cagtgcgcca aacagtaccc
    31261 acggacgaaa tttaccccag cgagagcgcg ttcgatcggc caacaacccc atgcaaggat
    31321 cggaaatcgc atccagtgca cgagcgacca aaaacatagt tccgacaaat ccggcaggaa
    31381 tgccaaaaat atcggtataa aagaacatca tatataacat tacgttatcg aaaataatgt
    31441 ggctggcggc gtctcccatg ccataaccaa ttttctcttt aacggacaac acttcactct
    31501 tcatctgctt ttccttgatg ctataggggt gctgagaccg ataacgcatt ttgtaaacca
    31561 tccctgtgag agcgaatatt ccgatatctg gttattaatt tatgattctt gttttatgtg
    31621 atcgtggtag cgttaattcc gctcatatat cattgtaaaa tatgggtttt atatgaacta
    31681 taatgctttc gtgataatac tctgcgtgta ttaggcggaa aaaactgatc tgggggatgt
    31741 agaaactcaa ggaagtagct ataatgcgcc ccgcctccat gtagcaatcg aggcgcggaa
    31801 gatcgtcgtc tccggtgagg cggctggact tcaaatccag ttggggccgc cagcggtccc
    31861 gggcaggttc gactcctgtg atcttccgcc aaaatgcctc ttcatacgtt ctccaacccc
    31921 tcgtaatact gtcaatccag atattttata ctgctcaata ttcaatcggc ttcaggaata
    31981 cctggtcaaa ggttttgtga tggacgatga gcagctgaag aatccgcctg tcggttcttc
    32041 tgtcctcgaa cgcatccgcg atattcgcgc cagcatgcgc cgtgtttatt tacaggtgat
    32101 tttcccccct gctgactctc agccaactgt atcgccatta aggtgaagat gttgttttac
    32161 acgctccaga acaccctttt tttatacgac tgttctattc ctttaacagc tggcaaaaaa
    32221 actgacctat aaacttcacc aataatcaga ctgccatcaa gattaatttg ttcatttaat
    32281 atatcaaatc aacttgtttt atttttacta tcaaaaatag ctcattgcct tactataatt
    32341 aatatataaa ttcggtgatt aattcttaac atgcttttgc taataatctc aattgctggc
    32401 cctataatat tgcgctagca ttgttttctg gttgtatcag cgatactcaa aaattcttaa
    32461 tacaatactc attcgactgg tacttatttg taactcagtt atattttgtc gcccggtgat
    32521 tcagaagaat gcaaaaaacg gctaccactc catcaaaaat acttgatctc actgccgcgg
    32581 catttttact tgtcgccttt ctgacgggta ttgcgggcgc tcttcagact cctaccctaa
    32641 gtatattcct cgcagatgaa ctgaaagccc gtcctataat ggtaggtttt ttcttcaccg
    32701 gtagcgctat tatgggaatt ctggtcagtc aatttctggc aaggcactcc gataaacaag
    32761 gcgaccgtaa attactgatc ctgctatgtt gcttatttgg agtgctggcc tgcacgcttt
    32821 ttgcgtggaa tcgcaactac ttcattctgc tctcaacggg cgtacttctg agtagttttg
    32881 cttccaccgc aaacccgcaa atgttcgccc tcgcccgtga acacgccgat agaacaggcc
    32941 gtgagacggt catgttcagt acatttttac gtgctcagat ctcgcttgcc tgggttatcg
    33001 ggccaccgct cgcttatgaa ctggcaatgg gatttagttt taaagtgatg tatctcaccg
    33061 ctgccatcgc atttgttgtt tgcgggctga tagtctggtt gtttttgcca tcaatacaaa
    33121 gaaatgttcc tgtcgttacc caacccgtag aaattttacc ctccatccat agaaagcgag
    33181 atacgcggct actttttgtg gtctgttcaa tgatgtgggc ggcgaataat ctctacatga
    33241 taaatatgcc gctatttatt attgatgaac tgcatctaac cgataaactg gctggagaaa
    33301 tgattggtat cgctgccggt ctggaaattc cgatgatgtt aatcgcaggc tattacatga
    33361 aacgtattgg caagcggcta ttaatgctca ttgctatcgt gagtggtatg tgtttttacg
    33421 ccagcgtact catggcgacg actccggcag ttgagctgga attgcaaatt ctaaatgcca
    33481 tcttccttgg tattctctgt ggtatcggca tgctttattt tcaggacctg atgcctgaaa
    33541 aaataggctc tgcgacaacg ttatatgcaa atacttcacg cgtcggctgg attatcgccg
    33601 gctctgttga cggaattatg gttgaaatct ggagctacca tgcgttgttc tggctggcga
    33661 tagggatgtt gggtattgcg atgatttgcc tgctgtttat taaagatatt tagctcacca
    33721 gtctgggcta agaaaaatcg gcgtggcgca aacagagcca tcctcgccat ttttgctatc
    33781 atgcctgcat acataaacga caaaacagta tgcagaggga aaaatgggtt ccaccagaaa
    33841 ggggatgctg aacgttctga ttgccgccgt gttgtgggga agttcagggg tctgcgcgca
    33901 atacatcatg gagcaaagcc agatgtcgtc gcagtttttg actatgacgc gtttgatatt
    33961 cgccggtttg attctgctga cgctgtcatt tgttcatggc gataaaatct tttctattat
    34021 taacaatcac aaagatgcca ttagtctgct gattttttcc gtggttggcg cgctaaccgt
    34081 acagctcact tttttgctaa ccatcgaaaa atcgaacgca gccacggcaa cggtgctgca
    34141 attcctctca ccgacgatta tcgtcgcctg gttctcactg gtacgtaaat cgcgcccggg
    34201 cattctggtt ttttgcgcta ttttgacatc gctggtcggg acttttttat tggtgacaca
    34261 cggtaatccg acgtcattat cgatctctcc tgccgcgtta ttctggggca ttgcctcggc
    34321 atttgctgct gcattctata ccacctatcc ctcaacgctg attgcccgct atggcacgtt
    34381 gcctgtcgtc ggctggagta tgctgatcgg cggtctgatt ctgttgcctt tttatgccag
    34441 acaagggaca aactttgtcg ttaacggcag tttgattctg gcgttttttt atttggtggt
    34501 cattggtacg tccctgacat ttagtctgta cctgaaagga gcacaattaa ttggtggacc
    34561 aaaagccagc attttaagct gtgcagaacc attaagtagc gcgctgctct ctttgctgtt
    34621 gctggggatc acattcacat taccggactg gctgggaacg ctgctgattc tgtcatcggt
    34681 aattttgatt tcaatggatt cccgtcgccg cgccagaaaa ataaatcgtc cagcgcggca
    34741 tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa gatggtttct gccgcttttg
    34801 cgacttcggg tgattgataa gattgcagaa attccttcac gttttctgca ttcttattat
    34861 cttcccgtgc caccaggatg ttcacatacg gcgaattttt atcttcaata aatacgctgt
    34921 cgtgcaccgg agaaagtccg gtctgctgaa tgtaagtggt gctaataatc gccacatcaa
    34981 ctttgggatc atccagtacg cgcgggagct gcgccccctc gagttccata atctgcaaat
    35041 gacgaggatt atcggttata tccagcgcgg taggtagtaa cccttttccc tctttcagag
    35101 taattagctt ctctttttgc aacagtaaaa gcgcgcgacc aaggttggtc ggatcgttgg
    35161 ggatcgccac tgtcgcgcct tcttttattt gcgccacggt ttttattttt ttggaataac
    35221 ccgccatggg gaagacaaac gtatttccca ccgctaccag tttataacca tgcgcctgat
    35281 tatcttgttc aagaaagggt cgatgctgga agacgttcgc atcaagctcg ccatgatttg
    35341 tcgcatcgtt cggtagcaac gaaccgctaa aacctaccag ttcaacatcg aggccatatt
    35401 tctcttttgc caccttttta gcgacttccg cgacatcttg ttctgcgcca tttataacgc
    35461 caactttaat gtgttttgca tcgctgctgc tctggtcgca acctgccagc agaattcctg
    35521 ccagcaataa tgcggccccc gcccgtagat gatgtgttgt cagtttcact tttttatcct
    35581 ttttttaatc attaactgac tataatggca agatcactac gatttaaaaa acgaaaagct
    35641 atcgaataga agaaaaaggt ataaaaaaac tggcacttct tactgaccac gcaaacacct
    35701 ttcgtacagc atgatgagag cggcaaaagc aaactacact tatgcttcat tcgatgcaca
    35761 tttgcagaag gttgcagcta tgaagccaac gatgctactc atgattaccg tattcctgac
    35821 ttttccagcc atttctcagg ccgagtcgcc attcagttcg ctacagtcgg caaaagagaa
    35881 aaccacggta ttacaagatt tacgtaaaat ttgcacgcca caggcgtcat tatcagacga
    35941 agcgtgggaa aaattaatgt tgtctgatga gagtaacaaa caacatatcc gtgaagctat
    36001 tgtggcgatg gagcgtaata atcagaataa ctactgggaa gcgctgggta aggtggaatg
    36061 cccggatatg taaatttcgc acacctccgc gctcaggcca gcagccgctg acttaagctc
    36121 gcacatcagt aaatcacctg ctttttcaaa ttccaatcaa aattgaacca gttcatcagg
    36181 attgtggata aatttccctc tacattattt tcatttcatc acatagaaaa tcttatctca
    36241 ttgaaacaat acttgttttt aatgcagaaa acgaagccca acggatgggc ataaaaatga
    36301 gcgaattttg atatcaccac cctctaaaaa atggtgaagt ctgagcaaag aatgttcgaa
    36361 aggagagagt agagatggaa agcgcaaatc tggatttaga agaaaataaa gaaattgcca
    36421 gtaagttcga acgtgcacta ggtatgggag caacacttgc agagctgcat ggcattactc
    36481 cggataccct tgaaggcgtt tatgcttacg cttataactt ttatgaaaaa ggaagacttg
    36541 acgaagctga actctttttt aaatttctct gtatttatga tttccaaaat tataactact
    36601 taaaaggata tgccgccgtt tgtcagttga agaaagatta tcaaaaggca ttcgacatgt
    36661 atcatatctg tctaatgcta tctcccgata acgatttctc tttggtttat tatatgggcc
    36721 aatgccaaat gggcttaaaa aatattaaaa tggccacgga gttatttaat acagtcgtaa
    36781 cctacagtca aaatgaaaaa gttaaagaga tggctactgc ttatcttgaa cttttgacag
    36841 ccaacaccga agaggaagtt caaacagagt gagtcagcaa aatgaagata aacggaaaaa
    36901 acgcttcttt tacgcctgaa gagctagcca gaaaatatca tgaggaagcc acgattgatt
    36961 taaaaaataa ccatgtcgat aaaataaatg agaagtataa caataaacac attacttact
    37021 ttcccgttga tgccatttta gatgggaata aacatttgcc atgagttaaa taaaaaagaa
    37081 ttaattacac ccggtgatag cttaaaaagg aggtgaatta tgtcggcacc gatcacggga
    37141 caaacgatca cgtttgaaca aatcagcgaa acattgcgta cacagtatag cgatgcggaa
    37201 aaaagactgc aggacagcag caaaacgcaa gttgatccca tgcggcttaa taaaaacccg
    37261 aaatcccttg ataacgatat tcgcgctcgt cttgaaaata aacctatgct tgctccgcct
    37321 gcaatgcagg tttcagattc agacaacgcc actacagcaa agacgaatga tgcccgcctg
    37381 acaatgattt tgggtaattt aacaggcatt gccgatcagg atattacaac acgattgcat
    37441 aataaccttg acagcaccct gcttcgacac gaaatggctc ataataagtt tcgtgaatta
    37501 agcgacgcct atgcatcctc attagattct gcgcaaaaag ctgacgatat catgcatcag
    37561 gccaacaata attacaatgc ggtcgataaa aaggtgcaat cgctggagaa aaaagtcaat
    37621 acattgaatc aagagctgtc gcaactgcaa cctggcgatc cgcaatataa taaagtgctg
    37681 acgcaaaaaa atgcggctga gaaaacactc acgctgtcat tacaaaaaaa atcgttagct
    37741 gagaaatcgt taaatacagc cattatggat gctgatgctg cgatcggcca aagtatggaa
    37801 atttttgccg aaattcaaca gcaagaacag attaataact ttaccactaa tatttgcctg
    37861 acccaggaaa accagaaaaa cagaaacgca acagccacat ttatcctttt gattacctca
    37921 gtaatggaag ttattggtga taccaattgc gaatccatta aaaatcaatc tgaggtaatg
    37981 aaagaaatta accatgtcag agaaaataaa ctcaatgaaa cagcgcgtag ctatactaca
    38041 tggacaaaac ttttaaacat tattaatgag gtcgttactg tagttacctt tgccgtaagt
    38101 gcagccttaa ttgtgattgg tattgtcgca gcagttccaa gtgctggggc aagctttgct
    38161 ggagcgctgg cactaattgg aggaatcgcg ggggcagtag tattaggtgt tgatattatt
    38221 tgtcaaatca ctctgggcac cacggctacc ggttggattt taggaaaact tactgaaggt
    38281 ctttcgggta ttatttcaaa atatgacccc acactactcg caatctgcgc tcttctggac
    38341 gccttgggtg tccaacaaac gactattgat ttagtacaag atatttactc atctgcagcc
    38401 gcttctattg ttatgataac attaatgatt ggtgcaacat taatttgttc tgtagcaata
    38461 agtgaacttg taaaggcact ctcaaaaact gccgctgaag aaataacaaa agaaataact
    38521 gacagtataa aatcaaccgt ggaatctatg atcaagtcag tttcaaaaaa aatcatgtca
    38581 gcattgaatt tcctcggtga caaaataaaa gcatcatcaa agtttataat gatatttgca
    38641 aaaataacta ttcttcttga aagaattgcc gcattctttt gcacaataat tacatcgagc
    38701 atgaatcaat acgctgccag taagtctgca gatatcgaaa ttctacaaca agatataagc
    38761 aatctatcaa aaacgcgcga acaaatgctt acagtgatgc aaaggataga taaaaccgtc
    38821 gaacaagagg tagctcagat ggtgcgtgta ttacaacacc gaactgaagc tttgaaattt
    38881 gcttctcatt ctatcgtata agaggattca aaaatgacca ataatattag caatataaac
    38941 aatacagctc ataataagaa tctcgacaaa acaacattcg atgaacaacc tggttatgaa
    39001 acaagagtat cacaagctaa aaaatactgc aaagtttttc gtcaaatgat attaaagttg
    39061 acacaaatac taaatttatt agtctgatag agtctgtccg tgctctctca cctgaattac
    39121 aatgtaaggt actagatggt cttaattatg ctaagaatac actacataat ataaatcaaa
    39181 attctgctgt agcaacaaaa tcattctgtc agtctaccga gtcaatagag aaaaccataa
    39241 catttaatat tctcgatatg ttagataatt tgacgatgag gcaacagaac attatggagg
    39301 cctttctaag agtctttaca tcaatgtaca atgcctctgc caaggctgat gctagcatga
    39361 gcatcgtttt tgaacagctt gtcaagcttc agagtgacga aacaaaaaat gcttcttggg
    39421 caaatttaac aggtgcaata gtaggctcag ctattaatgc tacgggagct gtagttatgg
    39481 caggtgcaga aattgcgaag cctgcgggaa agatagatga tatcgcagaa aaccaggaac
    39541 aaaatacaga caaaccagat gaccaacctc ggctaaagtc aaaagaagat ctggcaaatg
    39601 cgaaaaccag acttgccgaa atagaaacac aacaaaaaga attatcaacg aaccaaaata
    39661 ttgaggataa tgacaaacaa gagcaatcaa gtaagttaca aaaagaaaaa gaaagtatca
    39721 ctaataaaat taaagacaaa gaaaaaatta atagtcacaa tagaagatat agtatttcca
    39781 gttctatttc tcataccaat aatctatttc agtctattgc aaatctatct caatacggcg
    39841 gaacagtggc agcagaacat actcgtgcag aaaaagactt ggctgagcaa gctaaaaatg
    39901 ttatgcattc agttatgagt agctatctgg atcaaattaa tcgtgatgtt caaggaagag
    39961 atcaggcact caatttatta cagcaaataa tacaaacaat tattgatact gatagcgcta
    40021 tcgttcaagg cattcgctca taaattattg caataatagt taattatatt taaggaggaa
    40081 tgactcatga ttaataaaga taataattta aatatagata ttctacgtca gcagaatgca
    40141 aacattcctc tcaaagaggc atcctcgcaa agtgaggagc ctcatcaact ggaaaacagc
    40201 aaaagcaagg gtaatttgca taacagcgcc agtaaggata ataatattgg cgctatgctc
    40261 aagaaagatg ttgagaaact atcaacttta gtcagtgaac gtagcccgcg aaatgaagac
    40321 tggaaatgcc agcacgacat acaacttaac ttcgttaaaa acatgctcag aaattttcca
    40381 actaagaacc tgtcagagaa aggatccgcg ctcttacacg aaagttattt gctgataaaa
    40441 aacgttctgc agcttacaga aaatgttaat actattgacg gaaagttaaa ggatcccgct
    40501 aaaatattga ccctgcacaa ctctgtgaga aaaaccctgg ctaacattgc tgacaacaca
    40561 accgggtcaa gtgcaatggc atcgaccaaa ggtaccttaa atcacgctat cgcttcttta
    40621 gatagttctg cgcctgaaat gagaagttca gcaaccagca gtactttaac tgccagtagt
    40681 tcaagcgaat ttaaaagtga taaatatata tgcagtgata ttgctgattt gatgagtgtg
    40741 ctcggtagtg actacctcga aatctatgca aatagcgttg agataatgag cgcatactgg
    40801 cagaacttca gtgaacatat ccaaagcaat atgggaaaat ggactcactc taataaaaaa
    40861 ggcgatgcga tagtttttga tgtcaatgca ttccaaaaag ccttgatgca tttttattat
    40921 atcgataaat atcctaatgg tgattttcat tatcattaca atccagacta tgttttatac
    40981 cctccggcac ctgctgacaa aattggtgtt ccactggaag aagcagaaaa atggtgcgcc
    41041 gctttaggac ttccggttat tccacccgac ccaaaacacc gcacaccttc tcctatagtc
    41101 gaagttgaac cgcaaggtag cggtctgtat gttattattc ccaaccctca gattattgac
    41161 agtatgagtc agtcttcaga ctcaatggta cacagggatg ataaagggaa ggaaaaaaat
    41221 atatctaaag aattcaccgg ctacgaaata agcacggctg aataccaggc atggctggcg
    41281 ggctataaca gccaggcaga aaacatgaaa accgacgtac aggttatcac gactaaatac
    41341 agtacagcca actctaccta cgatacgatt atcaaattat tatcttcaac cattactgcg
    41401 ttgtttgatt cagcgaagga ttatttacgc ttttgattaa gtaacaccca caattatcgt
    41461 ccactatctg gtcccgtaaa atgggaccag cgtttcatat tattaagcat cagctttcat
    41521 tcatttcttc atcacaatct cacaaaatga gcgttttttg atagagcaat tctgccaata
    41581 ctttatttta gacagatcaa attaatacta tgaatagag
//
LOCUS       DAZFFG010000025        41038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFFG010000025 DAZFFG010000000
VERSION     DAZFFG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.025"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..120
                     /locus_tag="ACHJ7J_001740"
     CDS             <1..120
                     /locus_tag="ACHJ7J_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4999986.1"
                     /translation="LSNEEANQLRISSLYRDMVRYAQYRFSKVLSEWTPDAWV"
     gene            403..2553
                     /gene="dinG"
                     /locus_tag="ACHJ7J_001741"
     CDS             403..2553
                     /gene="dinG"
                     /locus_tag="ACHJ7J_001741"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO4999987.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2581..3543
                     /gene="ybiB"
                     /locus_tag="ACHJ7J_001742"
     CDS             2581..3543
                     /gene="ybiB"
                     /locus_tag="ACHJ7J_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO4999988.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3684..4769
                     /gene="hcxB"
                     /locus_tag="ACHJ7J_001743"
     CDS             3684..4769
                     /gene="hcxB"
                     /locus_tag="ACHJ7J_001743"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO4999989.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            4836..4895
                     /gene="ybiE"
                     /locus_tag="ACHJ7J_001744"
     CDS             4836..4895
                     /gene="ybiE"
                     /locus_tag="ACHJ7J_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO4999990.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(4998..5258)
                     /gene="ybiJ"
                     /locus_tag="ACHJ7J_001745"
     CDS             complement(4998..5258)
                     /gene="ybiJ"
                     /locus_tag="ACHJ7J_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO4999991.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5524..5790)
                     /gene="ybiI"
                     /locus_tag="ACHJ7J_001746"
     CDS             complement(5524..5790)
                     /gene="ybiI"
                     /locus_tag="ACHJ7J_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO4999992.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(5864..6541)
                     /gene="ybiX"
                     /locus_tag="ACHJ7J_001747"
     CDS             complement(5864..6541)
                     /gene="ybiX"
                     /locus_tag="ACHJ7J_001747"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO4999993.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHLQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6583..8865)
                     /gene="fiu"
                     /locus_tag="ACHJ7J_001748"
     CDS             complement(6583..8865)
                     /gene="fiu"
                     /locus_tag="ACHJ7J_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO4999994.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTNDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9130..9390)
                     /gene="mcbA"
                     /locus_tag="ACHJ7J_001749"
     CDS             complement(9130..9390)
                     /gene="mcbA"
                     /locus_tag="ACHJ7J_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO4999995.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            9666..10592
                     /gene="rlmF"
                     /locus_tag="ACHJ7J_001750"
     CDS             9666..10592
                     /gene="rlmF"
                     /locus_tag="ACHJ7J_001750"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO4999996.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(10589..12814)
                     /gene="ybiO"
                     /locus_tag="ACHJ7J_001751"
     CDS             complement(10589..12814)
                     /gene="ybiO"
                     /locus_tag="ACHJ7J_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO4999997.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13075..13797)
                     /gene="glnQ"
                     /locus_tag="ACHJ7J_001752"
     CDS             complement(13075..13797)
                     /gene="glnQ"
                     /locus_tag="ACHJ7J_001752"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO4999998.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(13794..14453)
                     /gene="glnP"
                     /locus_tag="ACHJ7J_001753"
     CDS             complement(13794..14453)
                     /gene="glnP"
                     /locus_tag="ACHJ7J_001753"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO4999999.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(14592..15338)
                     /gene="glnH"
                     /locus_tag="ACHJ7J_001754"
     CDS             complement(14592..15338)
                     /gene="glnH"
                     /locus_tag="ACHJ7J_001754"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO5000000.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(15742..16245)
                     /gene="dps"
                     /locus_tag="ACHJ7J_001755"
                     /gene_synonym="pexB"
     CDS             complement(15742..16245)
                     /gene="dps"
                     /locus_tag="ACHJ7J_001755"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO5000001.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(16544..17431)
                     /gene="rhtA"
                     /locus_tag="ACHJ7J_001756"
     CDS             complement(16544..17431)
                     /gene="rhtA"
                     /locus_tag="ACHJ7J_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO5000002.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            17666..17731
                     /locus_tag="ACHJ7J_001757"
     CDS             17666..17731
                     /locus_tag="ACHJ7J_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000003.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            17784..18299
                     /gene="ompX"
                     /locus_tag="ACHJ7J_001758"
     CDS             17784..18299
                     /gene="ompX"
                     /locus_tag="ACHJ7J_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO5000004.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18348..19931)
                     /gene="opgE"
                     /locus_tag="ACHJ7J_001759"
     CDS             complement(18348..19931)
                     /gene="opgE"
                     /locus_tag="ACHJ7J_001759"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5000005.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDNLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20203..20331)
                     /gene="mntS"
                     /locus_tag="ACHJ7J_001760"
     CDS             complement(20203..20331)
                     /gene="mntS"
                     /locus_tag="ACHJ7J_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO5000006.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20517..20984
                     /gene="mntR"
                     /locus_tag="ACHJ7J_001761"
     CDS             20517..20984
                     /gene="mntR"
                     /locus_tag="ACHJ7J_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO5000007.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20981..22099
                     /gene="ybiR"
                     /locus_tag="ACHJ7J_001762"
     CDS             20981..22099
                     /gene="ybiR"
                     /locus_tag="ACHJ7J_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO5000008.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(22157..23077)
                     /gene="ldtB"
                     /locus_tag="ACHJ7J_001763"
     CDS             complement(22157..23077)
                     /gene="ldtB"
                     /locus_tag="ACHJ7J_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5000009.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23296..24888
                     /gene="ybiT"
                     /locus_tag="ACHJ7J_001764"
     CDS             23296..24888
                     /gene="ybiT"
                     /locus_tag="ACHJ7J_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO5000010.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25129..26394)
                     /gene="ybiU"
                     /locus_tag="ACHJ7J_001765"
     CDS             complement(25129..26394)
                     /gene="ybiU"
                     /locus_tag="ACHJ7J_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO5000011.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDNIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(26546..27361)
                     /gene="ybiV"
                     /locus_tag="ACHJ7J_001766"
     CDS             complement(26546..27361)
                     /gene="ybiV"
                     /locus_tag="ACHJ7J_001766"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO5000012.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLKSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKIARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(27507..29939)
                     /gene="ybiW"
                     /locus_tag="ACHJ7J_001767"
     CDS             complement(27507..29939)
                     /gene="ybiW"
                     /locus_tag="ACHJ7J_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO5000013.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREIAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYHRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPEA
                     QDDIIARTEHML"
     gene            complement(29945..30844)
                     /gene="ybiY"
                     /locus_tag="ACHJ7J_001768"
     CDS             complement(29945..30844)
                     /gene="ybiY"
                     /locus_tag="ACHJ7J_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO5000014.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTLALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            30975..31637
                     /gene="fsa"
                     /locus_tag="ACHJ7J_001769"
     CDS             30975..31637
                     /gene="fsa"
                     /locus_tag="ACHJ7J_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5000015.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMAITAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(31713..32462)
                     /gene="moeB"
                     /locus_tag="ACHJ7J_001770"
     CDS             complement(31713..32462)
                     /gene="moeB"
                     /locus_tag="ACHJ7J_001770"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO5000016.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(32462..33697)
                     /gene="moeA"
                     /locus_tag="ACHJ7J_001771"
     CDS             complement(32462..33697)
                     /gene="moeA"
                     /locus_tag="ACHJ7J_001771"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO5000017.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPACQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGR"
     gene            33901..34866
                     /gene="iaaA"
                     /locus_tag="ACHJ7J_001772"
     CDS             33901..34866
                     /gene="iaaA"
                     /locus_tag="ACHJ7J_001772"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5000018.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            34853..36724
                     /gene="gsiA"
                     /locus_tag="ACHJ7J_001773"
     CDS             34853..36724
                     /gene="gsiA"
                     /locus_tag="ACHJ7J_001773"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO5000019.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGSGHYVAQPQSEYALMRR"
     gene            36744..38282
                     /gene="gsiB"
                     /locus_tag="ACHJ7J_001774"
     CDS             36744..38282
                     /gene="gsiB"
                     /locus_tag="ACHJ7J_001774"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO5000020.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAIAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFHDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAEEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            38300..39220
                     /gene="gsiC"
                     /locus_tag="ACHJ7J_001775"
     CDS             38300..39220
                     /gene="gsiC"
                     /locus_tag="ACHJ7J_001775"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO5000021.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGGSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            39223..40134
                     /gene="gsiD"
                     /locus_tag="ACHJ7J_001776"
     CDS             39223..40134
                     /gene="gsiD"
                     /locus_tag="ACHJ7J_001776"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO5000022.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            40312..>41038
                     /locus_tag="ACHJ7J_001777"
     CDS             40312..>41038
                     /locus_tag="ACHJ7J_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO5000023.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSE"
BASE COUNT         9792 a        10190 c        11053 g        10003 t
ORIGIN      
        1 ctgtctaatg aagaggccaa ccagttgcgc ataagttcgt tatatcgtga tatggtccgc
       61 tacgcacaat atcgtttttc taaagtttta tccgagtgga cgccagacgc atgggtgtaa
      121 agatcgcagc agaataatgg cattgatggc cctggctcag gccagggcgg gcaataccga
      181 tttctgcgaa gcgatgacgg tggcaagtgt aaccacgaaa tgcggtatca cttgctgttt
      241 tctgccctga agactggcct cactgtgccg tcgaactttc aatcaaatat attgcgagag
      301 ggcgtgtttg cgagccgctt tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa
      361 tgccacaata ttggctgttt atacagtatt tcaggttttc tcatggcatt aaccgccgcg
      421 cttaaagcgc aaattgccgc ctggtataag gcgcttcagg aacagatccc cgactttatt
      481 ccccgtgcgc cgcagcggca gatgattgcg gacgtcgcca aaacgctggc cggagaagaa
      541 gggcggcatc tggcgattga agcccccact ggcgttggga aaacgctctc ctatttgatt
      601 cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg tggtgagcac cgccaacgtg
      661 gcattgcagg atcagatcta cagcaaagat ttaccgctgc tgaaaaagat cattcccgat
      721 cttaaattca ctgccgcttt tgggcgtggg cgctacgttt gcccgcgtaa tctgacggcg
      781 cttgccagca ctgagcccac gcaacaggat ctgctggcgt ttcttgacga cgaactgacg
      841 ccaaataatc aggaagagca aaaacgttgt gcgaagctga agggcgatct cgacacttat
      901 aaatgggatg gtctgcgcga tcatacggat atcgctatag atgacgatct ctggcgtcgt
      961 ttaagtaccg acaaagccag ctgcctcaac cgcaactgtt actactatcg cgaatgcccg
     1021 ttttttgttg ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg
     1081 gtgatggcgg cgatggaaag cgaagcggta ttgcctgacc cgaaaaattt actgctggtg
     1141 ctggacgaag gccatcacct gccagatgtg gcgcgggatg cgctggagat gagtgccgaa
     1201 atcaccgcgc catggtatcg gctacagctg gacttgttca cgaaactggt cgctacctgc
     1261 atggagcagt ttcgcccgaa gaccatcccg ccgctggcga tccctgaacg tttgaatgcg
     1321 cattgtgaag agctgtatga gcttatcgcc tcattaaaca acattctcaa tctctacatg
     1381 cctgccgggc aggaagcaga acaccgtttt gcgatgggcg aactgcctga tgaagtgctg
     1441 gagatctgcc agcggctggc aaaactcacc gagatgctgc gtggtctggc ggagttattt
     1501 cttaacgatt taagtgagaa aaccggcagc catgacattg tacgtctgca tcggttgatt
     1561 ttgcagatga accgcgcgtt ggggatgttc gaggcgcaaa gcaaactctg gcggctggct
     1621 tcgctggcgc aatcttccgg tgcaccggtg accaaatggg cgacgcggga agagcgcgaa
     1681 gggcagctac acctctggtt tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg
     1741 ctgctgtggc gcagtattcc gcacattatt gtcacctccg caaccttgcg ttcgctgaac
     1801 agtttttcgc gtttgcagga gatgagcggg ctgaaagaga aagcgggcga ccgttttgtg
     1861 gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc
     1921 gttgagcctt ccatcgacaa cgaagagcag catattgctg aaatggcggc ctttttccgt
     1981 gagcaggtgg agagcaaaaa acatctcggt atgttggtgc tgtttgccag cgggcgtgcg
     2041 atgcagcgct ttctcgacta tgtgacggat ttacgtctga tgttgctggt gcagggcgat
     2101 cagccgcgtt accgtttagt tgaactgcac cgcaaacgcg tcgccaacgg tgagcgtagt
     2161 gtgctggtgg gcttacagtc atttgccgaa gggcttgatt tgaaaggtga tctgctcagc
     2221 caggtgcata tccacaaaat cgcttttccg cctatcgaca gcccggtggt gatcaccgaa
     2281 ggggaatggc tgaaaagcct caaccgctat ccatttgagg tgcaaagctt gccgagcgcc
     2341 tcgtttaacc tgattcagca ggttgggcga ctgattcgaa gtcacggttg ctggggcgaa
     2401 gtggtgattt acgataaacg cttgctgacc aaaaattatg gcaagcgact actggatgca
     2461 ttaccggtat ttccgataga gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa
     2521 aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa
     2581 atggactatc gcaaaatcat taaagagatc gggcgcggga aaaaccacgc gcgcgattta
     2641 gaccgggata ctgcgcgcgg tctgtatgcc catatgctca atggtgaagt ccctgacctt
     2701 gagttgggcg gcgtattgat tgcgctgcgt atcaaagggg aaggggaagc agagatgctc
     2761 ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg
     2821 atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggccaacct gacgccgttg
     2881 ttggcgattc tgctgcacaa actcggtttt cctgtggtgg ttcacggggt tagcgaagat
     2941 ccaactcgcg tgctgacaga aaccattttc gaactgatgg gtattacgcc aacgttgcac
     3001 ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc agtcggtgcg
     3061 ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt
     3121 gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gcgtctttcc
     3181 agcgtttcgc atccggaata catcgggcga gtcgcgaagt tctttagcga tatcggcggg
     3241 cgggcgttac taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgccca
     3301 cagatcaatc tcattgatcg tgaagagatg cgggtgctgt atgaaaaaca ggaccctgct
     3361 ggaagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg gattcagcgt
     3421 tgccttgctg gccgcgaacc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg
     3481 gtggctacgg gtgaagcggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt
     3541 taattctttt ctcagccgga tgagccatgt catccggctt ttaccccacc gcaatatgaa
     3601 attcctgcat ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca
     3661 cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc
     3721 acagctttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag
     3781 ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttcacatggt attggcatga
     3841 tcccaagcta tgtgcgctcc tggagtcagg ggcatctgca aattaaccat catgccaaaa
     3901 tcgttaaaga ggcgggggcg gcggtcacgc ttgatggcga tcgcgcattt ggtcaggtcg
     3961 cggcacacga agcgatggcg ctggggattg agaaagcgca tcagcacggt attgccgccg
     4021 tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgtgcag
     4081 cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcaccgt
     4141 tccacggtcg cgacagccgc tttggcacca atccgttctg tgtggttttc cctcgtaaag
     4201 ataatttccc gctgttgctc gattacgcca ccagcgccat cgcatttggt aaaacccgcg
     4261 tcgcctggca taaaggcgtc cctgtgccgc caggttgcct gattgacgtt aacggcgtgc
     4321 cgacgaccaa tccggcggta atgcaggagt cgccgttggg ttcgctgttg acctttgccg
     4381 aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttccggcg
     4441 gtaaaacgac gcatcaggaa acgttacaaa ccagtcccga tgccattctt aactgcatga
     4501 ccactatcat catcaatccg gaactgttcg gcgcgccgga ttgtagcgcg cagaccgaag
     4561 cctttgccga gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctcccgg
     4621 gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg
     4681 gaagctggca ggccatttgt gatgcggcgc ggcagattgg tatgccggaa gagacgttgc
     4741 aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa
     4801 caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg
     4861 gaaatacttg tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta
     4921 ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcgtaaacgc
     4981 cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg
     5041 gtggcggaag tgatgctata tccactggca ccggctgccg ccgctttctc agccagtttc
     5101 gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg
     5161 ttctgtgcct gggatgccgt taccggttcc gcggcaaaca cgccaaatga cagagttgaa
     5221 agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt
     5281 ttttgtttaa gtgacgttgt ttcgtcgatg tgatgagtat cacgtttttt tacgagagat
     5341 aaaatcgaag agaattgacc gccttgttca aataaattga ttgatatcta acatactgat
     5401 aaaccattta tttgttgtac ttatggaccc attttatatc gcgtggcagt cacgacagcg
     5461 ttaaaggcac ggaatgccac gcaaaataca aattatactc gctatacatc tcgccagttg
     5521 tagttaacgt aactggctgt ctttcgaacc tctgcgatta tatcctgtat aagctggttt
     5581 ttgtaaatct ttctcctgct gacaatgaat acataagcgc acgccaggaa tggcttcccg
     5641 acgggcttgc gggatggggg caccgcactc ttcacattca tacaggcttt cgccacgcgg
     5701 aatttcaccc cgagcgcggg caatcgcatc ttcaattgta ctgttgatct gttcgttgac
     5761 ggcgtcatcg ttagcccaac cggatgccat atcgtcctcc ccatatcaat gcttgtacag
     5821 ttaagtgtag ctaatatagg gacgaactcg ggtagttcaa gcgtcagatc tccgaccatt
     5881 cccgcagcag attatgataa agattgagca gcgacaggat ctcttcactt tcgccgtagc
     5941 ggcttttcag cgactgaata ttgttgtcca gttcaaacag catggcgcgc tttttatcat
     6001 cgcggatcat cgactggatc cacataaatg atgccactcg tacgccgcgg gttacgggtg
     6061 tcacgcaatg caagctgctg gagggataca acaccagatc acctgccggg agttttaccc
     6121 gatgttgtcc gaaggtgtca ttaacgacca gttcgccgcc gtcgtagctt tgtggatcgc
     6181 ttaaaaacag cgtggcagaa aggtcagtgc gcatccagcc gttttgtaga tggctgcgta
     6241 ctgcgccatc cacatgaaaa ccataggttt cattgttctg atagcgatta aacagcggcg
     6301 tggaaagggt acgcggcaag gccgcggcaa agaacaaagc atgttggtta accgcgttca
     6361 gcacctcatt ttgcagggcg gcgtataacg tgctgcgggt gtctacctgt tgattgttct
     6421 taacttgcgc accttgtgcg ccggtggtga cgcgtccatc cacccattcg gcttgttcca
     6481 gttgttcgcg aaaacgggcg acgtcctgtg gcgataacac gccgggaatg tggtacatca
     6541 tacaagttct ccaaaaagtg gggcctgcgc cccacatctg aatcagaaat gcatattggc
     6601 tgtgagcaag aaggttcttg gctcgcccgg gtgataacgg tagccgctct tattgattga
     6661 ggcgacgtaa tcggtatcaa acaggttgta gacgtttagc tggaagtcga gattgcgatt
     6721 aacccgatac cccagtttgg catcggcgac ccagtaacct tcggtaaacg ctggcgttcc
     6781 caccgcgccg tctgaacctt tatgcatact gccgatatag cgtgcgcccg cgccaacaga
     6841 gatatcatcg gttgcctgat attggctcca taaggtgaag gcgtgctccg gggtatacgg
     6901 cagcgatgag gaaccatcct gggcaacatc tttgccgttt ttgatggttg ctttttgctg
     6961 ggtatagccg ccaatcacct gccacgcggg agtaatattc cctgccacgg atatctcata
     7021 gccttcgacg cgtttcttac cgtattgcga gtaagtcccg tcatcatttt gctcaacttc
     7081 attttcgata tcagtacgga acagcgcggc ggtgagcaac aggcgtttat ccagaacctg
     7141 ccatttggtg ccaatctcgc tggtgttggc tttttgcggt ttaaaatcgg tgcggttggc
     7201 actgttaccg ctgccagact gcgcaagggc gaagttgttg ccgcccggag gctgctggga
     7261 aacggcatag ttaatataga cattgccgtt ttccgtcagg tgatacagcg ccccggcttt
     7321 ccagttcacc agattgcccg acttggcggt gtcgacggtg gtgaccggag aaccttttgc
     7381 cacaccagct gggcaggtga tggctccgcg tccgctgccg ccgcaggcgg tggcactgtc
     7441 atattcagta tgataattat ccagacggat cccgccgttc agctcaaaat cacgggtgat
     7501 ttgcagcgta tcgaaggcgt aaattgcgaa ggtatccgtc tgaccattgg cgtttgcgcc
     7561 gttgcgcgtc aggccgccgg gatgaatgct gctgtcagga tgataaatat ttaccgcagg
     7621 taacgtcacc ggattaacgc cgtagttagt ctgcgtttca cgggtaaatt ccacgccggt
     7681 actgacatca tgaccgatag aaccggtata gaacgtcgag gtcaggttgg tctggttggt
     7741 gagaatttta ttactcacat ctttggtatt cgccgtgcgt gaccacgtcc agctattcac
     7801 atcgctggtg ggctgggtaa tattcgacgc cccacccata atcgccgtca tcaggtaatc
     7861 ctgcttcacg cgcgaccaac gggtagtgtt gcgaatggtg gtgttatcgt tgatgtcgtg
     7921 ctcaaaacgc atggtggcgg tgtcggtggt cgaatcgtcg taatcggaat ccgtgccgta
     7981 aaagttatgc gtatcaactt ttccggaatg attcagggtc gccgttcctg cagatggggc
     8041 agaatagccc ggcaaaccga tggttggaat gccgccgtct ggcgtgttgt gctgggtgac
     8101 atgcagataa ttaagataca aacgattcgc tgtaccaagg ccaaaagcga cagaaggggc
     8161 gaccccgtaa cgctcatttt tgactttgtc gcgtccggca tcgttcgttt tttcgcccat
     8221 cacattcagg cgcaccgcgg tggtatcacc aatgacctga ttgacgtcca gtgtgccgcg
     8281 gcggaaccag gcgctgccaa tactggcgga ggcgtcaatg ccggaatcat tgcgcggctg
     8341 cttgctgatc atattgatcg agcccgtcgg cgcgctgcgc ccgtagtcgg tgccggaagg
     8401 ccctttaatc acttcgacct gctcagtatt gaaggtgtcg cgcgagacgc tgccgatatc
     8461 gcgaatgcca tcaatataaa tactgttaga ggtatcggca ccgcgcatat aaatggcgtc
     8521 gccagtggtg gagttaccgt tctcacccgc aaaaaacgca cccacgccgg ggacgttttt
     8581 gagcgcgtcg gtaaggttgg ttgcgccctg atctttaatc acttgttcag aaatcaccgt
     8641 catcgtgcgg gtagtatccg ctaccggacg cgagaatttc ggatctgcag attgttgtgg
     8701 cgcataaagc gaaggcgttg atgcttcgac aaccagcgtg tcatccgcgt tagtttgccc
     8761 ttcggcggcg agtgcctgag ccacaggcgt gatgccaata caaagaccgg caaagaacgt
     8821 gagcgaatga aattgtctgg cagggaaatt gcgattgttt tccattttgc aggtgacttt
     8881 ttcttatatg ttgaaatttt cactgccacg agaaagcctc tctgcggatg cgatgccact
     8941 gttaatctcg tgatagtgtt cgtgcaagtg ataataattt tgataagcat tatcacttgg
     9001 cgcgattttc tatcacccat tgcgcgaaaa agaaatacat ttatttacat tgcgttcata
     9061 tttgatgttt tttgattaca aaacaggcga aaactgcatc cagaggcgaa tgcagttacg
     9121 ttggtggggt tatttgtaga tggttgcagt accgaacatc tggtcatttc cgcctgcgga
     9181 attaatgaca taacctttcg cgccttgctc gcgcgctttt tctgccagtt tatcctccag
     9241 atcgcttagg tttgatgcgc cagttgccga aacggtaccg gcgggccgta gctgaccgct
     9301 gtccaggtta ctcatcggtt gcgcggcata agccgtgaga gagattccgc tcaggacggt
     9361 ggcaatcagt agtgtgaggc actttttcat aatgacatcc tcataaatac aacgggtagt
     9421 gcctgataag tgtaggtcgc ctgggcgatg aaaatgacgc aaaaaattga cagagatacg
     9481 cttatttttt gaacgatata tttttacaaa ataagacaaa tcaatgacat gcatgatgct
     9541 gatttttatg atgaattgcc ataaaccata gccatgttaa ggtttttaga aatgataaac
     9601 gcagccagat atttaccctg tcgcgttatg cgggtatctt acgccgctgc ttaaaggaga
     9661 atgctatgtc cgcccagaaa ccggggttgc atccgcgcaa ccgtcatcac agccgctacg
     9721 atctcgccac gctttgtcag gtgaatcctg aactcaggca attcctcacg cttacacccg
     9781 ccggggagca aagcgtagac tttgccaatc cgctggcggt gaaggcgctc aataaggcgt
     9841 tgctggccca tttttacgcc gtagcgaact gggatatccc cgacggtttt ctctgcccgc
     9901 cagtaccggg ccgggcggat tatattcatc accttgccga tttactggct gaagcaagcg
     9961 gaacaattcc agctaatgcc agcattcttg atatcggcgt tggtgcgaac tgtatttatc
    10021 cgctgattgg cgtacatgaa tatggctggc gttttaccgg tagtgaaacc agcagccagg
    10081 cgttaagcag cgcgcaggcg atcatcagtg ctaatccggg gcttaatcgc gccattcgtc
    10141 tgcgtcggca aaaagagagt ggggcgattt ttaacggcat catccataaa aacgagcaat
    10201 acgacgcgac cttgtgtaac ccgccattcc acgattccgc cgctgcggcg cgggcaggta
    10261 gtgagcgtaa acgccgtaac ctggggctga acaaagacga tgcactgaac tttggcggcc
    10321 agcaacagga gttgtggtgt gaaggcggtg aagtcacctt tatcaaaaag atgattgaag
    10381 agagcaaagg cttcgcgaag caggtgatgt ggtttacttc actggtatct cgtggtgaaa
    10441 acttaccgcc gttgtatcgt gccctgacgg acgtgggcgc ggtgaaggtg gttaaaaaag
    10501 agatggccca agggcaaaag cagagtcgct ttattgcctg gacctttatg aacgacgagc
    10561 agcgccgccg ttttgtcaat cgccagcgtt aaagcgttgg ctcccccggc ggtaacggtt
    10621 cagccggggt cgcgcccgga gcaggcagca cctgataagt ctgcaccggc gcgcgaacgc
    10681 ccgccaggtc gaaatgtttt ttcacctggc tgtcgagggc aaagcgtacc gtccactgtt
    10741 tgagtggcag cgtggtgaac gaaacacgca gtgtaaacgc ggtattgctt aagccgacaa
    10801 tcccggcaaa gttcggttca ccaataatca gcccacgaat ttcttcgttt tccattaatt
    10861 ccgctaccgc atctttcagt gcctgattgg ctttatcagc atcttcatgg cgatcaacat
    10921 cgtagtttgc cactaccgaa ccaatgccgc ggacaaagtt ggcaaaggtg gtaatcgaag
    10981 accacggaat gatgtggtac gccccggtat cctggcgtac gcccacggag cgaatcgaca
    11041 tccgttccac agtgccggtc aacggcccaa tagtcaccaa atctccagtg ttcatgccgt
    11101 tttcaaactg aataaatacc ccggtgataa tatctttcac cagcgtttgc gaaccaaacg
    11161 agatagccag acctaatgcc ccggcacctg ccagcaatgg cgcgatattg acgccgattt
    11221 ccgacaacac aatcatgatg gtgatggtac tgataatcac cgccagcgcg ttacgaaaca
    11281 gcgtaagcag ggtacgcgtg cgggcgctgg gtagcgggcg gccatgaata tccgaagcca
    11341 gccggttttc gatcaaactg gcgagcaccg tccagccaac cgccgagaag aaaagaatga
    11401 gtgcgatacg gatcaggata tctacggttt tctgccccgc gccgttttgc agccagttcc
    11461 agaaatcgaa caatccccat gcgctcaaca gcagcattac cgccacgcag acggtcagaa
    11521 tacgcgccgt tttcagcgcc gccgaaagcc agccattcag ccgtttttgc aactccggat
    11581 agttacgctg agtatgtggc gagagagtga tggtttttgc cagccagcgg gaaaacatac
    11641 cggaaacaaa tgctgcgata ccaataatcg ccaggctgcg cactgttgca cccatcataa
    11701 attttaggct gttgcccgga tcgaacaacg aaaagaaaaa cagcacgata aaataggcgc
    11761 ttgccagcca gtgccacacc agcgcaaagg cgcggataaa caggctgaaa aaggccagcg
    11821 aatgctccgc aaagttgagc aaatgctggg taatctcttt tttattacga aagatcaggt
    11881 acaacgccca gacggtcatg cacagcataa tgatgacgtt cgccagcgca cctatctgta
    11941 cattcacctg attagagata atcggcacgg ccacaatcag gccataacct atcaaactgc
    12001 ttaaccagct caggcgacga ctccagtaac gggcgctctc gtcctgaatc gtgaacgggc
    12061 gcagttccgc cacgtttggg caaaaaatca ggcgtagtac ggctttgaaa aattcaatca
    12121 gggcaaaggc gttgagaaac aaactttgtt ggaaagcgat ggtgcgactg cctgcattca
    12181 ggttgtcgct taatacctgg ccgacaaata atgtcagggc cagtaacagc aggtcgataa
    12241 taaacgcccc gataatcatc gccggaagct gcaaccagtt actgcgctca cgattttttt
    12301 gccgcgccca ctgacccatt ttgcgataca gcggcagtgc gcacaggcga atcagccagt
    12361 aaaaaccaaa cactaaaacc gctaacattg aaaaatgggt cagcgcattg ctgaaggttt
    12421 gcggattaaa cggcttatgc ggggagccgg tgatattgcg ataaagttgc ccgaagcggg
    12481 cggaaagggc ttcaccataa tggcggctga cttcggtgac cttttgcagc acggtttgct
    12541 cttcaaccag cgtcggcggc acgatcttcg ggaccggttc agcaggtggc gtcgcggcga
    12601 cggtgcgcaa ctggtcgatc aactctttac gcgaggtgtc attatccagc acatccgcca
    12661 gtgcgccata ggccgctttt ttttgttcga tatccggttc cggggccggt tcagtcgttg
    12721 agtccgttgt cgttgtggtt gtaacgccgg gtatggatac cgcgtgggca ggtgcgccca
    12781 gcaggcagaa gaggatgaac aggatccacc gcatgactcc tccagtgaga aaacagagca
    12841 aaaagataag tatagatgct ggggaagggg cgattttaaa tgagaggaat ctggtgtgcc
    12901 tccctttcgg gtgaaaggga ggaagggata ttaagcaggt tgctgacaac gtgcgggctt
    12961 ttttgccgga tgcggcgtga acgccttatc cgtcctacaa gttcatgcaa ttttaataag
    13021 ttgccagata tcctgtaggc ctgataagcg tagcgcatca ggcaatgtgt cttattaaga
    13081 gacgtgctgc aaaaattcct gcaagcgctg gctcggcggg ttcttgatca acacctgcgg
    13141 atcgccatct tccgcaatcc ggcctttatc aatgaagatc aggcgcgaag ctactttctc
    13201 ggcaaaaccg atttcgtggg tcacgatcac catcgtcatc ccttcttcag ccagatcctg
    13261 cataaccttc agcacttcat ggcgcagttc cgggtcaaga gcggaagtcg gttcatcaaa
    13321 cagcatcatt ttcggcttca ccgccagcgc gcgggcaatc gccacacgct gctgttgacc
    13381 accagaaagt tcggaagggt agtgatgtgc acgttctgcc agaccgactt tcgccagcag
    13441 ctcacgcgcc agtttttccg cctcttcttt gttcgcgcca cgcacgcgta gcgggccaaa
    13501 catgacgttt tccagcgccg tcagatgcgg gaaaaggtaa aactgctgga acaccatacc
    13561 tgcttcctgg cgaatcaggc gctcgtcaac tttcggatcg ttaaccttca ggccatcgac
    13621 aatcagatcg ccggaggtga tttcttccag tttgttgatg cagcgcagca gggtcgattt
    13681 accggaaccg gacggcccga taatcaccac gacttcgccc tgggcaatgt tcaaatcgat
    13741 attgtgcagc acctgggttg ggccaaagtg cttggaaacg tttttaaatt caatcacagg
    13801 attttcatcc ttctttccag acgacgcaga ataaagctca gcaccagggt aataatcaga
    13861 tagaacaccg ccacggcgct ccagatctca agggcgcgga agttaccggc aataatttct
    13921 tgcccctgac gggtcagttc cgccacgccg ataacaataa acagcgaggt gtctttaatg
    13981 ctgatgatcc actggttacc cagcggcggc agcatacgac gcagtgccag cggtaaaatg
    14041 acgtagcgaa tggtttccca acgtgaaaga ccaagcgcca gtcctgcttc acgaaaacct
    14101 ttgtggatag acagcaccgc accacgcgtg atttccgcaa tgtacgcgcc ggagttgatc
    14161 atgatggtga ccaccgccgc agtaaatggg tcgatgcgta agtcgttaaa cgccatcggc
    14221 agggcgaaat aaataaacat cacctggacg acgataggtg tgccgcggat cacttcaata
    14281 aagaccagcg cgacgtggtt ggctatccaa ccaccgaagg tgcgtgcaaa acctgccagc
    14341 aatccgatta ccagaccgcc agctagaccg aggaccgaaa tccaaagggt cattttggca
    14401 ccttcaatca gaagcggaat ggcaggccag atggcactcc agtcaaactg catatgttgt
    14461 tcctgttacc gtggtgtaaa taatcaaatt cagggtaatt aatggccccg gagcgggttt
    14521 gcgcccctca ccctaatcct ctccccagag gggagaggga actgccagtg cgctttacag
    14581 gtgtagcgtt attatttcgg ttcagtaccg aaccattttt tgtagatttc gttgtaagtt
    14641 ccgttctcgc gcagggtttt caacgcgccg ttgactttgt cacgcagctc gtcgctacct
    14701 ttcgggaacg caataccgta ttgctgcgct tccagagagt cacctaccgc tttgaactga
    14761 ccgttaccgg cggttttgat gaagtacaga atgtttggcg tatcgtgcag aacggcgtct
    14821 gcgcggttgg tgcccagttc catataggcg ttatcgatgt tcgggaactg acgcagatct
    14881 ttagttttga tgtttgcttt cgcgtaatca acggagccag tgccgctctt caccgcaacc
    14941 actttcccgt cgagatcttt cacgcttttc acatcgttat tgttagcttt caccatcact
    15001 aacaggccgc ttttgtagta gccgtcagag aaatcgatcg cttttttacg ctcgtcggtg
    15061 atggtaatgc ccgccagcgc cagatcgacg tttttggttt gcagtgccgg aatgatccca
    15121 ctgaaatcca tcggcttcag ttcgtaatcc agcttcagct ctttagcgat ggcagcccac
    15181 agatcaacgt caaagcccac atatttatcg ccctgtttaa attcaaacgg aacgaaggcg
    15241 gtatccgtcg cgacaactaa ttttttatcc gcggcatgag aagaaaccgc aaaagccagg
    15301 gtcagtgcag ccagtgaaac ttttaataca gacttcatag catttccttt tttctatcca
    15361 cgggacgatc ccctgcgtga cattcacata tatgaaaaaa tcgtgccagt tttgcaactc
    15421 attgattttt cgagatgcgg caaaaacgtg atgcacgatt tatagggcaa taccctgaag
    15481 atgcaccatt ctggggcacc aaactggtgc gctaaaattg tgcactcaac acagtgatta
    15541 tttagcgtaa aacctgatga aaaaacaatc tttatgtaac gattgtgtga tgatgtggat
    15601 acaaaaaatt tacttaatca gctggggata gcagatggat gcactaaata agtgcgttga
    15661 ggcgggctgc aaaacaaaac ggcctcctgt caggaagccg cttttaccgt gtactaaagt
    15721 tctgcaccat cagcgatgga tttattcgat gttagactcg ataaaccaca ggaatttatc
    15781 caggtcgcga gacgcggcgg tcaggatatc tgcggtgtcg tcatctttcg cttcgccaat
    15841 cgctttgcgt acgtcattag cgacgattgc gtaacggtca gccagttctt tcaggtgatc
    15901 ctgaacgttg tggatgtcca gcgggtaact tttcagcggg gttttgctgt tgataacttg
    15961 agtggtcccc agagctacac cgcctagctg cactgcacgt tctgccatgg tatccagatg
    16021 atcgatcagt gcagtgcgga agccatccag catttcatgt acggcaataa agttagcgcc
    16081 gcgcatgttc cagtgcgctt gtttggtaat caaagaaaga tcaataaact ggataacctg
    16141 gcgattcagc aactctactg ttgctttttt ctcgctgtca gagacatcgt tgcgggtata
    16201 aagcagattg gtcgcttttg atttaactaa tttagcggta ctcataattt catatcctct
    16261 tgatgttatg tcccagtaat taacgagact aagtatagca ccggctatgt gttccgctat
    16321 tctggctgtt cctatcacac taatagtggt aacaagcgtg aaaaacaaaa ctaataaaaa
    16381 ttcaatgagt tagatatatt gataagaaca attcttattt atttgctcgc caggaatttt
    16441 cggaataaat atgcttttgc ataattcgat atataaagac ggtgtagagg aaaagtagcg
    16501 agaaattctg catggttatg cataaccatg cagaaatgta aatttaatta atgtctaatt
    16561 cttttatttt gctctctttg cgtactgtca gcgtagaacc cattgaagcg gcgatgatag
    16621 cgccgagcgc cagcagctgt atgggtgtca gtgtttctcc gaggaaaatc atcccggaaa
    16681 cggcagccag cgccggttcc atgctcatca gcgtaccaaa tgtccgtgtt ggcaaacggg
    16741 tgagggcaat catttccagc gaataaggca gagcggtcga gagaatagcg accgccagac
    16801 ccaatggaat aaccgaccag tgccagagtg cttcaccagc ctgaagcgct ccaattggca
    16861 cgaaaattaa cgctgcaatc aacgaaccaa ttgccaccgt cgcagggcca tgttccgctc
    16921 ctgcgcgttg cccacttaaa atgtaaatag cccaacaagc cccggccccc agtgccagcg
    16981 cacagccggt taaatcgaca tgggaaacgt cttgccccag aggtagcagg aaccacagac
    17041 caagaaccgc cagcacaacc cagacgaaat ctaccgggcg acgagaagag aacagcgcca
    17101 ccgccagtgg tccggtgaac tccagcgcca ccgcaatacc cagcggtacc gtctgaatag
    17161 aaagataaaa aagataattc atccccccca gcgaaacgcc gtaaaacaac agcggtaatc
    17221 gttgctcttt ggcaaagcgt agtcgccatg gcttaaagaa cgcgatgagg atcagcgttc
    17281 ctaatgccag acgcagcgca gtgacacccg gtgcgcccac cagaggaaaa agtgacttag
    17341 ctaacgaagc tccaccctga atagacgcca tggcaacgag caatatgact attggtaacc
    17401 agaccggcat tttacgtaat gaaccaggca tcctttctcc cacaaatatc tagacttaag
    17461 taaagcgtgg agtgtactgg atatacccaa tgctggttga gcatttgttg aaaaaatttt
    17521 cccccgtttt gactaaaatg cgccaggatt gatggaatca ttagtctggt gattaggaat
    17581 aatctggatg aatgacaggg aaaacatgcg taatacttac gcagttctct gaaaaagtga
    17641 tttaaattta gatggatagc ggtgtatgga aacgttctgt tacatgaaat ggcccgttag
    17701 acatcacaaa tcgcgaagag tttcccatta atttttgata tatttaaaac ttaggactta
    17761 tttgaatcac atttgaggtg gttatgaaaa aaattgcatg tctttcagca ctggccgcag
    17821 ttctggcttt caccgcaggt acttccgtag ctgcgacttc taccgtaact ggcggttacg
    17881 cacagagcga cgctcagggc caaatgaaca aaatgggcgg tttcaacctg aaataccgct
    17941 atgaagaaga caacagcccg ctgggtgtga tcggttcatt cacttacacc gagaaaagcc
    18001 gtactgcaag ctctggtgac tacaacaaaa accagtacta cggcatcact gctggtccgg
    18061 cttaccgcat taacgactgg gcaagcatct acggtgtagt gggtgtgggt tatggtaaat
    18121 tccagaccac tgaatacccg acctacaaac acgacaccag cgactacggt ttctcctacg
    18181 gtgctggtct gcagttcaac ccgatggaaa acgttgctct ggacttctct tacgagcaga
    18241 gccgtattcg tagcgttgac gtaggcacct ggattgccgg tgttggttac cgcttctaat
    18301 cacttcggtg atataaaaaa tccgcctctc ggggcggatt ttttgtttta aggtttcggg
    18361 tcgaaaatat cggttccgag atggttgtaa tccaccttct gtaactggaa gttggtgatg
    18421 tatattggcc cggctttctt ctcagatata aacgggtatt tgatgttaat ttccttcgct
    18481 ttaattcccg tccactgcga gaaaaagcct aagaagtcat tggctgagcg gcgggcttta
    18541 atcacccgat gcgctttatc gtcgctggaa atgaccataa aaggtacctg gaaattttgc
    18601 tgatacttat catcatgggc aaggtattgc acgtctttgc cgcgctcttt aaaggccaga
    18661 ccgtggtcag aaaagtaaac cagcgagaag ctgctgccgc tgttgcgtaa ctgatcgtac
    18721 agcttgcgca gtaaatcgtc cgtttgcgtc atagtataga gatagcacga cgtttctttc
    18781 gattgcacaa aggtttcgta tttgccttgt gtccggtcgc aggcctgcgg atgcgagccc
    18841 atcagatgca gaacaatcag ctgcggttgc gagtgctctt gcgccagcac ttgagcggtc
    18901 atatccagta acgcttcgtc tttcgtgttt ttatctgctt caaaattacc ttctttcagg
    18961 aagtacactt catctgcacg tttagcgatg ctggcgatag cagtatcgta ttcgccgatt
    19021 tggccctggt tggaaaacca ccaggtctgg aagcccgcgc gatttgccag ggtgacaaag
    19081 ttatcctgaa actgtggttt gccatcgact acgcgattga gcgttaagcc aagcgatttc
    19141 tgcgtggagc cactggcggc aatgtagtca gcaaatatca aaccgttaac gctgctggca
    19201 aacggggtat tgtcccagtg accgccaaag gcaccgaggg cgtcgcgacg cgcgctttca
    19261 ccgatcacca ccacataggt ctgatacttc ggcttaacgg cagtgaccgt ccaggtatct
    19321 ttcattccgg aaagttttgc catccgttct tgctcttcaa tcacctcgtt attattgacg
    19381 atgacatcct tagcaaaacg aaataccgga tagccagtat ctttcagttt aaatacgcca
    19441 ccccaggcca ggttttgcac gggagcaaca aaaaatgtcg ccacactgaa taccagacac
    19501 aggttgtcga aggtattcca gcgcgctttt tcattctctt tttttcgcct gattgctatt
    19561 acgccgagcg caaaaataaa taagccgacc aggtagctgt accacggaaa aatcgtcagg
    19621 atttcggtcg actcttccat attggtggag tgcaatgcca gcaatgtatt gaaattaggc
    19681 gcgccgtagg cctgagcaaa agggaaatag caagccgcca ccagcgaact gacaccgacc
    19741 agaacttttt gtacgcgagg caatgttcgc catagcaaaa gcaaaatagc cgtaaacgca
    19801 gcggtgtaga gcaaactgaa ggggtaacct aagccgaggt taattaatag cgactgtaaa
    19861 aagtagaacg cagtccacgg gctaaatacc cggctacggg taacaagcga ttctttgagg
    19921 gttaaattca tatgccacta tcgtaacaaa cgccatgtgc ttaccctggc gtcaagggtc
    19981 aatacctgca agagtgcgtg aagaggggaa aggggtccgc atccgcgagc cgcaatatac
    20041 gcagggctgc aagaagatag agcgagccgc cgttcaggtc aactactgac gaacaaatct
    20101 tttgcgaagg ggattgcaaa tccgacaaaa agtggggttt ttcagaccaa aacgcgttat
    20161 gcctgcatga aagcaggagg gaagcggcgt accgcgacgc cgctatttat cggaaggttt
    20221 atcttgctgc ggtttgttgt tgaccatatc gcacaacata gagagcagca ttaaccgtac
    20281 tttaaaggga gaatgactaa acacgcgcat acacctcttg aactcattca taagacctcc
    20341 tgacttgcta atcccgtcga tccttgaggg atgattgcat tacatacaga tatagcacag
    20401 gctatattat atagctattg ctaaaacgtt aattttttgt accttcgcaa ctctggttta
    20461 caatgtgcgc acgaaatgag aacgctaggt atgcgtcacc ataatgagga agcacaatga
    20521 gtcgtcgcgc aggtacgcca acagcaaaaa aagtgacgca gttagtgaac gtggaagagc
    20581 acgttgaagg gttccgccag gtcagagagg cgcatcggcg cgagcttatt gatgattacg
    20641 ttgagctgat ttctgacttg atcagggaag tgggggaagc tcgtcaggta gacatggctg
    20701 ctcgtttggg agtttcgcaa ccgacggtgg ctaaaatgct taagcgactg gcaactatgg
    20761 ggctgattga aatgatcccc tggcgtggcg tgtttttaac ggcagaagga gagaagctgg
    20821 cgcaggaaag ccgcgagcga catcagatag tcgaaaattt cttgttggtg ttgggcgtca
    20881 gtccggaaat cgcccgtcgc gacgcggaag gcatggagca ccatgttagt gaagagacgc
    20941 tcgacgcttt tcgtttgttt acccagaaac acggtgccaa atgagcctgc cttttttacg
    21001 cacgctgcaa ggggatcgtt tttttcagtt attaattctt gttggtatcg gattaagctt
    21061 tttcgtgccc tttgcaccga aatcctggcc tgctgctatc gactggcaca ccatcatcac
    21121 attaagcggc ctgatgctgc tgaccaaagg tgtggagtta agcggttatt ttgatgtgct
    21181 ggggcgcaaa atggtgcgcc gctttgctac ggagcgtcgg ctggcgatgt ttatggtgct
    21241 ggcggcggcg ctgctttcta cctttctgac caacgatgtc gcgctgttta ttgttgttcc
    21301 gctgactatc acgctaaaaa gactgtgtga gatcccggtt aatcggctga ttatttttga
    21361 ggcgctggca gtcaatgctg gttcgctact gacgccaatt ggcaacccgc aaaatattct
    21421 tatctgggga cgttctggtc tttcgtttgc cggatttatt gcccaaatgg caccgctggc
    21481 tggtgcaatg atgctgacac tcctgctgtt gtgctggtgt tgtttccctg gaaaggcgct
    21541 gcaataccat acgggggtgc aaacgccgga gtggaaaccg cggctggtgt ggagttgtct
    21601 gggactgtat atcgtctttc tgacggcgct ggagttaaaa caagagctgt ggggactggt
    21661 gattgtggcg gtgggctttg cgctgctggc acgtcgcgtg gtgttgagtg tggactggac
    21721 gctgctgctg gtgtttatgg cgatgtttat cgacgtccat ttactgaccc agcttccagc
    21781 gttgcaaggc gtgttgggta acgtgagtca tttatctgaa cccggattat ggttaacggc
    21841 aatcggttta tcgcaggtga tcagtaacgt gccgagtacc atattgttgc tgaactatgt
    21901 gccgccgtct ttattactgg catgggcggt aaacgtaggt ggctttgggt tattacccgg
    21961 atcgctggca aatttgattg cgctacgtat ggcgaacgat cgccgcatct ggtggcgttt
    22021 ccatctctat tcaataccga tgctgttgtg ggcggcgctg gtgggatatg ttttgttggt
    22081 tatgatcccg gcctggtaag gtctggcaaa taaaaaaggc ggattatgag tccgcctttg
    22141 ttttacgttt tgacgattaa ttcagacgaa ccggcatccc ggagcggttt ttaatcgctt
    22201 catcaagaac aacctggtca acatctggct gaccggtcac tgtctgcacg ctcttcgtca
    22261 gggtaattgg cacaatttcc tgaccttcaa actgggcttc ggtggtagac agcggattat
    22321 ggacttcaat ataacggctg ccgtctggct cggtggtcgc ttttaccggc tcatcaataa
    22381 actgtacgcg ggtaccgacc ggtactttct cgaacaggaa tttgatgtct tcgttacgca
    22441 gacgcacaca accatgactt acacgcaggc cgataccgaa gttggcgttg gtgccatgga
    22501 tagcatacag gcgaccgatg tagagtgcat acagccccat cgggttatcc ggacctgccg
    22561 gaacgacagc cggaagcggt tcgcccgcag cgcggtactc tgcgtgcatt ttggcggtcg
    22621 gcgtccaggt cgggcctgct ttcttacgct caactttggt ggtccagttg ataggcgtat
    22681 ctttgcctaa ctgaccaatg ccgatcggca gcacgataac ggtgttggtc cctttcggat
    22741 agtaatacag acgcatctct gcactgttaa tgacgatgcc ttcatgaaca gtatccggca
    22801 ggatcagctg ctgcggaatg ttcagtacag taccgccttt cggcaggaag gtatccacac
    22861 ccgggttcgc ttccatcata ttggaaagcc ccatctggta ctccgcggca aaatactcca
    22921 gcggctgagt gttaccttca ggaatggtga tcacctgatt ctgaccaacc aggcgactcc
    22981 cgtcggttgg cagaggataa gttaccgcag aggcggtact gcaaaagccg acaacagcga
    23041 aggccgctgc gaataatgtt ttcaatttca tattcatgtt aagcgagatt ttgtgcctgg
    23101 caggccattg ggttgagaat attagagtat tggaagcgca ttataagttc attccagctc
    23161 acagtgaaat cagatgtgta cgaaatcaca ttttttgcct ttggcttgag tgtagacctt
    23221 aagcgaggag caggatcttc tttcagactt atggcataat gcgcggtttg tcatatctct
    23281 tttcaggata cgcctgtgtt agtttccagt aacgtcacca tgcagttcgg cagtaagccg
    23341 ttgtttgaaa acatttccgt caaatttggc ggcggcaacc gttacggcct gattggcgcg
    23401 aacggtagtg gtaaatccac ctttatgaag atcctcggcg gcgacttaga gccgacgttg
    23461 ggtaacgttt ccctcgatcc caacgagcgc attggtaagc tgcgtcagga tcagtttgcc
    23521 tttgaagagt tcactgtgct ggatacggtg atcatggggc ataaagagtt gtgggaagtg
    23581 aagcaggagc gtgaccgcat ctatgctttg ccggaaatga gtgaagaaga cggctataaa
    23641 gtggccgatc tggaagttaa atacggcgaa atggacggtt actctgcgga agctcgcgcc
    23701 ggtgaattgt tgcttggcgt gggaattcca gtggaacagc actacggccc gatgagtgaa
    23761 gttgctcctg gctggaagct gcgtgtgctg ttggcgcagg cgctgtttgc tgatccggat
    23821 attctcctgc tcgacgaacc gaccaacaac ctcgacatcg acaccattcg ctggctggaa
    23881 caggtgctga acgagcgtga cagcaccatg atcatcatct cgcacgaccg tcacttcctt
    23941 aacatggtct gtacccacat ggcggatctg gattacggcg agctgcgcgt ttatccgggt
    24001 aactacgatg agtacatgac ggcggcgacc caggcgcgtg aacgtctgct ggccgataac
    24061 gccaagaaga aagcgcagat tgctgagctg caatctttcg ttagccgctt tagcgccaac
    24121 gcctcgaaat ctcgccaggc aacttcgcgc gcgcgccaga ttgataaaat caaactggaa
    24181 gaggtgaaag cctccagccg tcagaacccg ttcatccgtt ttgaacagga taagaagctg
    24241 ttccgtaacg cgctggaagt ggaaggtctg accaaagggt ttgataacgg tccgctgttt
    24301 aaaaatctca acctgctgct ggaagtcggt gaaaaactgg cggtactggg taccaacggc
    24361 gtcggtaaat caacgctgtt gaaaacgctg gtgggcgatc tgcaaccgga cagcggcacc
    24421 gtaaaatggt ctgaaaacgc gcgcattggt tactatgctc aggaccacga atatgagttt
    24481 gaaaatgatc tgaccgtgtt cgaatggatg agccagtgga agcaggaagg cgatgacgag
    24541 caggcggtac gcagcattct cggtcgtttg ctgttcagcc aggacgacat caaaaagcca
    24601 gctaaagtgc tttccggtgg agaaaaaggg cggatgctgt ttggtaagtt aatgatgcag
    24661 aagccgaaca ttctgatcat ggacgaaccg accaaccacc tggatatgga atccatcgag
    24721 tcactgaaca tggcgctgga actgtatcag ggcacgctga tctttgtttc acacgaccgt
    24781 gagtttgtaa gctccctggc gacccgcatt ctggaaatca ccccggaacg cgtgatcgac
    24841 tttagcggta attacgaaga ttacctgcgt agtaaaggga tcgagtaagt gattatccgt
    24901 caccccgata ctgtcggggt gacggcggct gatgacaaat gcaaaatagc ctgatgcgct
    24961 acgcttatca ggcctacgca gtacctgtaa tatattgaat ctgcaggact ttgtaggcca
    25021 gataagtcgt taacgccgca tcaggcatta agaaagcgca cttgtcagca atctgagtaa
    25081 cgtcgaatcg attttcttaa tcccacatca attcacttcc attaactctc aaacatccat
    25141 gcccaacgct cgcttaccgt gaatgttgag gtcggcaagc gtaaagcgtc cttcccagtt
    25201 tgtttcatag tcttcgcgcg ggaagtcgcc cggcgatgcg cctttttcca gtgcggcctt
    25261 caccttgtgc gcgtaggcaa gatttttctc gcacatcggt gccgcaggaa tgtacatcac
    25321 gttgccccag ccctgttgat tttcaacggg ggcaacggaa tggatgacgt cgcagtgcca
    25381 ccagacggaa tcccctgctg ttatcgcggg aatggaagtc agcgctttaa gcagtagtgg
    25441 atgccatttc tctgaaatgg gcagaacttt gccaggtgca accccgcata gttcatcgtc
    25501 cggtacgtca tccagcagcg ggcgcaataa aacatacgcc atcgcttccg ggataggcac
    25561 cacatgtaac aatccctgac ccgataacat atcagagagc gccgtccagc cttgaaacgt
    25621 gcggaacacc gaacattttg tagtgttatc taccgtgtac tcttcaactt ccgtacgatg
    25681 tgccgcatgc cagggatcgt atttcgccag attgccatta aagacgttag cgaaaacgtg
    25741 ctgatacgct ggaagcagcc agcgttccag cgccccggag tcggtatgcg ctccaagacc
    25801 tttggaggtc gttccgggcg gacggcggcg gatacgatca gggtagatga cgctcacatc
    25861 cgggttaaac cattgctttc catcactttc aaatgtccac agacgattga gaaacgactg
    25921 cgcattcgcc atttcttcac tctggcgggc ctgcatttgc gcctgcgacc agtagatggg
    25981 gtaaatctcg ggacgtgaag cgctgagcgt cccgaagaaa ttatcgccgg ggcctttgta
    26041 gacctcgtca aagcggttgc ggtccagata gtccagcatc gactgatccc agcctagcgc
    26101 ttgttcgcgg gggaaatggc cttttatcac cgcacaaccg cgacgtttaa tctgttcgcg
    26161 ctgctctgca gtaacatgac ctgctttgat atcggcataa gacagtaccg gccagacggc
    26221 atcgccctgt gctttgagtg cgttgatttc agccactcgc gtggcaatgt tatcgcttag
    26281 ctgattaaag atctgctgga cgtcgccaag ctgcgcccgc agcgcgtgct tcatctgacg
    26341 gatagctgct ttgtgatcgg caggcaatgt gtcgctggta aaagtagaag ccataaccac
    26401 ctcgcattaa ctttcattcg aaactatgat gatttgcatg tgacaatata agttataaat
    26461 aagttaatgc aagtttaatg atttgatgtg atgtacagga tgtaaaaaga agggaaaacc
    26521 ggagcgcata gctccggtag agaggtcagc tgttaaaagg cgatgtgtta tccagtacag
    26581 cctgaatcac attcagcgcg ccttcatgat tattatcatc ggtagcgtaa cgggcgattt
    26641 gtttaatgtt ttccgcagca ttgcccatcg caaaggaata acgcgctatt ttcagcatct
    26701 ccgcatcgtt accgctgtcg ccaatcgcta ccacattttg cggtgacaga tcccagcgtt
    26761 tcagtaaccg cgaaataccg tttgctttat gtagaccggg aataatcagg tcgataaagc
    26821 caaaaccact ggtaacgggt ttcataatgc catccagcgc tatgtgcagt ttgtcgatca
    26881 ctaacgggat ttgttcatcc ggcaggttga gtgaaaactt gaacagtacg tcgtcaatct
    26941 cctgataatc ttttacagct ttcaggcgat ggtagtgttt tgccatcagt gcgacgaatg
    27001 cttcgggggc attttcgctg acatatgcac ttttcagacc gcaggcgaca aaattgagtt
    27061 gcttatcttt tagcaattcg ccaatcacaa tccgcgattc atgtcgggtc agttcgccgt
    27121 ggaacaactg cttgccatgt tcgtaaacca gtgcgccgtt ttccgcgaca aaagagatct
    27181 catcctttag ctcaggaaag aacgaaataa gctggtaata ctgattaccg ctggcgacaa
    27241 cgaactcaat gccgcgcttt ttcagttcct gatattgcgc cataaaacgt ggttggttgt
    27301 acgttttggc gtcgttaaga aaagtaccgt ccatgtctgt gacgataact tttacgctca
    27361 taggtgtgct cctggctcga aaatgaaacc gtaacagtgt aataacaatg tgacgcagag
    27421 cacaaattat atttcgaatg aaagtaagga tgaaattgat gatgtgaatg atttagcccg
    27481 gcggcgacgc cgccgggctg agaagattac aacatatgtt cagtacgggc gatgatatcg
    27541 tcctgtgcct ctggagagag cgcggtgaag aaggcggaat accccgcgac acgcaccacc
    27601 agatcccggt attgatccgg atgttttttc gcctccagca atgtttcgcg ggaaacgatg
    27661 ttgtactgaa tatgccagcc tttatgcact tcaaagaagg tgcgcagcag gatcatcagc
    27721 ttctgcttat cagattcgtt ctccagcgtt gccggattca gtttctggtt gagcaacacg
    27781 ccgccgagaa tcgctgccgt aggcagttta cccactgagc caatgactgc cgttgggccg
    27841 agatggtcag taccggaggc cgggcttgcg ccttctgcca gcggggtatg cgctttacgt
    27901 ccatccggcg ttgccatcgt ctgtgcgcca aacggtacgt tagcggagat agacgacgta
    27961 cccgcgtaat agttgccgcc aaccggacca cgaccgtagc gcgggttatg gtactgtttc
    28021 agttcgtcga tataggtctg ataagcgcga gccagcagcg tatcgacagt atcatcgtcg
    28081 ttgccgtact tcggcgcacc gttaatcagc cgctgacgca gttgctcatg agtcaggccg
    28141 tcgaagtcat ccgccagtgc ggcagccagc tgttgctgac caatcgcgcc ctgttcaaac
    28201 accagtttct tcactgccgc caggctgttg ccgaggttgg caatgccaac ctgcaggcca
    28261 gaaacccagt catatttcgc gccgccttgc ttgatacttt tcgctcgctc aatacagtca
    28321 tccaccagcg ccgagcagag aatatcgtgc acgttctctt ccagcatggt gtcgacgaca
    28381 tattcgattt cgattgattt acgcgtgtag taacggattt gcgtatccca cgcgtccatc
    28441 acttcatcga agttgttgaa gttacctgcc gacaacgctt tttcttgtgg caggaacact
    28501 ttgccgctgg tggcatcacg cccgccttcc agcgccgcca gcatcacgcg ggcgaagttg
    28561 ataaagctca tgccggtaca gcgatagccc catttgccac cgacggcggt ttcgatacaa
    28621 ccaatcgctg cgtagtcata agcgtcctgc ggttcaatac cgagtttaat aaattccggg
    28681 atcacgattt cgtcgttgtt gaaggcgggc atcccgaagc cgcagcggat cacctgtacg
    28741 caggcgtcga ggaaatcgtt gctcattccc gcgtggtaac gcacgctgag gttaggctga
    28801 gtggaacgca ggcgaccgca ggattcgagg atcgcgtaag agaggggatt caccgcgtcc
    28861 attggttgac catcaaccag attttgcccg ccaatggtga cgttctgata cagcggactt
    28921 cccgcagagg cttttgagtg tgagccggag cggattttgt tcacttccag caatttcaac
    28981 cagcagctat gcagcatctc gatggcgtgt tcgcgatcca gtgtctggtt cagttcaacg
    29041 tcgcggtgat aatacgggta cagatactga tccatacgac caaaagatac tgagtgaccg
    29101 ttagattcga tctgcaaaat caactggatg aagtaacaca gttgcagcgc ctgccagaac
    29161 gtctgcggcg gctggtgggc gataagatcg cagttttctg ccattgccag cagttcatcg
    29221 cgacggcttt cgcgggtttc ggtcgcggct atttcacgcg ccagggcagc gaaacgttca
    29281 atgtgttcac tgactgccac cagcacgata tcaatcgctt tcaggaattg ctcgccatgt
    29341 aaatcttcca gcaccgtcag attgatgcgc gagcgacgtt ccgctacttt ctcgcgcaga
    29401 ccatcaagac ctttttccag cagcagcggg aaattgaccg ccaggtgcgc atcgccggag
    29461 gtcatattgc cttccgcttt aatgatgccg gtcgccagca gacctttttg ctcatcggta
    29521 aacatgccgt agcagcgatc ctgtacggtc tgaccgcgcc accacgggca cacttcatgc
    29581 agaacgcgtt tgttctcttc gctcaccgca aagccagcac cgggacgatc tgccagatca
    29641 tcaatctctt tttcgatcca cgagacagta tattccggga agatcggcgc ggcgcgaact
    29701 tcgcttgcct ggttgccgat gatcaactca tcgtgcttga tccagatagt gcgattcgcc
    29761 aggtgatgtg ccagcgccag cgcgcgacgt acagggatcg gcttatcgag atgttgttga
    29821 tacatctcgg tatagtgctg cgcgcgctcg gtacaaaccg gcggtttcac aatatgcacc
    29881 agtgcatttt tgtgcgcttt aatgcggtcg ctgagcgtgt ccagtttcaa tgtggtcatg
    29941 gttgttatcc tcgtaaggtc gcggttaacc ctttctggca ggcatactgc tgggcaaagt
    30001 cgagcagttc tggcgcatca agcggttttt ccggggcgtc atagggcaga ttaagtaagt
    30061 gatatttgtt gatgcccagc gtgtggtagg gcaggaaatg aatttcgcca acgtgcagct
    30121 cgtcggcggc aaaatcggta atggctttca cagaggtttc gtcggcatta aagccctgaa
    30181 tcagcggcac gcggataatg atttttttgc ccgctgcggc gagttttttc aggttatcca
    30241 gcactctggc ggcgttaccg tcggtccact gtttaaacgg cgcgtcggca acgtgtttta
    30301 aatcggcaag aaacagatcg atatagggca gagaaggggc gatatatttc cacggcacat
    30361 gcagacaggt ttctaccgca gtatgaatgc cggcctcgtg gctggcttgc agcagcgccg
    30421 tcgccatttc cggctgcata aagggttcac cacccgaaag cgttaagcct ccgccgctgc
    30481 gatcgtaaaa cggtttatcc cgcaaaacgg tcgccatgat ctcctcaacg cttttcactt
    30541 caccacacac ggttaatgcc agtgtcgggc agcagtcggt taacgccgtc agatgctccg
    30601 gggttaactt ttcccgatga ataagcaaac cattcagcgc gcgctcaatc acttccggcg
    30661 ctgccttagc gcacagctcg cagccttcca gacacagtcg tgcgtcatac agcagatcct
    30721 gcgtgcgggc gcggctttcc gggttctgac accagcggca gcccagcgaa cagcctttaa
    30781 gaaatacgac cgtgcggata ccggggccat catgggtcga gtagcgctga atattgaaaa
    30841 tcatagttgc ctctctgttt cgttcaaaca ttaaaatact tttgaatgaa agttagattg
    30901 atgtgcgtca actgttcaga gagttttccc gtgatagtct acattcagac aaaaagtaca
    30961 ttttgaggat ggttatggaa ctgtatctgg atacttcaga cgttgttgcg gtgaaggcgc
    31021 tgtcacgtat ttttccgctg gcgggcgtga ccactaaccc aagcattatc gccgcgggta
    31081 aaaaaccgct ggaggttgtg cttccggaac ttcatgaagc gatgggcggt caggggcgtc
    31141 tgtttgccca ggtaatggct atcactgctg aagggatggt gaatgacgca cgtaaattgc
    31201 gttctattat tgcggatatc gtggtgaaag ttccggtgac cgccgagggg ctggcagcga
    31261 ttaagatgtt aaaagcggaa gggattccga cgctgggaac cgcggtatat ggcgcggcac
    31321 aagggttgct gtcggcgctg gcaggtgcgg aatatgttgc gccttacgtt aatcgtattg
    31381 atgctcaggg cggtagcggc attcagactg tgaccgactt acaccagtta ttgaaaatgc
    31441 atgcgccgca ggcgaaagtg ctggcagcga gtttcaaaac cccgcgtcag gcgctggact
    31501 gcttactggc aggatgtgaa tcaattactc tgccactgga tgtggcacaa cagatgatta
    31561 gctatccggc ggttgatgcc gctgtggcga agtttgagca ggactggcag ggagcgtttg
    31621 gcagaacgtc gatttaaccg gacgttctgc atcctcataa attgctgatg acgtggcgga
    31681 gtgccgcgtc ttatcaggcc tggaggtggc aattactgcc cgcacacttc acaccccgga
    31741 ttgcgcatca gtttcatttc gcgaaactga caggtcatcg catcgtacat cacgattttg
    31801 ccgctggccg gtttgccata acctgccagc agcttgatcg cttccattgc ctgcaacgaa
    31861 ccaattacgc cgatcaatgg tgccatcacg cctgcttcta cgcaggttaa cgcgttctcg
    31921 ccaaacaaac ggctaaggca gcgatagcac ggttcgccgt cctgataagt gaagaccgtg
    31981 atttgacctt ccatacgaat tgccgcgccg gaaaccagcg gcaccttcgc ggcaaaacag
    32041 cctgcgttca gttgattacg taccgcaacg ttatccgtac agtcgagcac cagatcgtgt
    32101 tcagcaatca atgctgcaag ttctgcgtca tccagcagtg cattgactgg cgtaatcgcg
    32161 atatgtgggt tgatccgcgt cagggcgtca cgggcggatt ccaccttcgg ttgcccgacc
    32221 gtagcatcac tgtgcagcgt ctggcgttgc agattcgaga gcgaaaccgt gtcgaagtcg
    32281 agcagcgtca ggttgccgac accggcgctt gccagatact gcgaggctgc gcagccgagt
    32341 ccaccgaggc cgactaccag cacacgagaa tcttttagcg cctcctgacc gtcaaaatca
    32401 aaaccgcgca gaatgatttg ccggttgtag cgcagcatct cctgatcgct gagttccgcc
    32461 attaccggcc tccgaacaac gcgttaaacg gttctacttc cacccattcg cccacttcca
    32521 cattgccgcg atcgcgttcc agtacgataa agcagttgcc gaggctaaag gagctaaata
    32581 tatgtgaacc ctgatgtcca gtggtcgtca cttccagttc gccatcggcg ttgcgttgca
    32641 gcacgccgcg ctggaaatca aggcgacctg gcgttttttt caggcgggac gctgtgcgta
    32701 cgcgctggca cgcgggcagg ccgctggcgg tgttaccgct tagttttgcc agcaaaggct
    32761 gtaccagttg atagaaggtc agcgtcgctg aaactgggtt gcccggcagg ccgcagaacc
    32821 agctattgct gagtttaccg aacgcgaacg gtttacctgg tttaatcgcc agcttccaga
    32881 aggcgatctc ccccagctct tcaagaatcg ttttggtgta atccgcttca cccactgaaa
    32941 caccgccgga actgatcacc acatccgcct ggctgtcggc ttcaataaat gcggcgcgca
    33001 gggcatgggg atcgtcgcgg ataatcccta agttgattac ctcgcatccc aactgttcta
    33061 acatcaggtg tacggcgaga cggttggtat cgtagatttg gccgtcgccc agcggctgac
    33121 cgggcaactg gagttcatca ccggtagaaa aaagcgctac acgcacttta cgaatcaccg
    33181 gaacttcggc aatccccagt gaagcaatta ctggcagctc tgcggtagtc aggcgagtcc
    33241 ccgccgggaa aacaaccgca cctgcagaga tatcttcacc gcgacggcga atattttgcc
    33301 cgctacgcac ttcagcagta aaacgcacgc cattgtccgt ttgttcagtc tgctcctgca
    33361 tcaccaccgc ttcgcagcct tccggcaccg gcgcaccggt cataatacga atgcaggtac
    33421 ccgcaggcca ttcaccatgg tatggctgac cggcaaagga tttaccggca acgggcagcg
    33481 gttgcccgga ggcaatatcg gctaaacgca ccgcgtagcc gtccattgcg gagttatcaa
    33541 accccggaac atcaagcggc gaaacgacat cgctcgccag aatacgacca aaacactgta
    33601 ccagtggcag cgtttcctgg gcggtcagtg gggtgacgcg agaaagcatc tcattaagcg
    33661 cggtgtcgag cgacatcaat ccggtggtaa attccatgaa aacactcctg cggaggcaaa
    33721 atcgaatttg cctattatgt cagaaaaacg ccacagactg tatgccacct cgggcgtagc
    33781 gctgggtcct gcctttacat gccatatcca tctttctata ttcaaaaatt gaatgagtaa
    33841 ttcataaaaa ttctgatatt tatagcaaaa gtggcgagcc acccttaatg gacgaatact
    33901 atgggcaaag cagtcattgc aattcatggt ggcgcaggtg caattagccg cgcgcagatg
    33961 agtctgcaac aggaattacg ctacatcgag gcgttgtctg ccattgttga aaccgggcag
    34021 aaaatgctgg aagcgggcga aagtgcgctg gatgtggtga cggaagcggt gcgtctgctg
    34081 gaagagtgtc cactgtttaa cgccggaatt ggcgccgtct ttacgcgtga tgaaacgcat
    34141 gaactggacg cctgtgtgat ggatggtaac accctgaaag ccggtgcggt ggcgggcgtt
    34201 agtcatctgc gtaatccggt tcttgccgcc cggctggtga tggagcaaag cccgcatgtg
    34261 atgatgattg gcgaaggggc agaaaatttt gcgtttgctc atggcatgga gcgcgtctca
    34321 ccggagattt tctccacgcc tttgcgttat gaacaactaa tggcagcgcg cgaggaaggg
    34381 gcaacagtcc tcgaccatag cggtgcgcca ctggatgaaa aacagaaaat gggcaccgtg
    34441 ggggccgtgg cgttggattt agacggcaat ctggcggcag ccacgtccac gggcggaatg
    34501 accaataaat tacccggacg agttggcgat agccccttag tgggtgccgg atgctacgcc
    34561 aataacgcca gtgtggcggt ttcttgtacc ggcacgggcg aagtcttcat ccgcgcgctg
    34621 gcggcatatg acatcgccgc gttaatggat tacggcggat taagtctcgc ggaagcctgc
    34681 gagcgggtag taatggaaaa actccctgcg cttggcggta gcggtggctt aatcgctatc
    34741 gaccatgaag ggaatgtcgc gctaccgttt aacaccgaag gaatgtatcg cgcctggggc
    34801 tacgcaggcg atacgccaac caccggtatc taccgtgaaa aaggggacac cgttgccaca
    34861 cagtgatgaa cttgatgccg gtaatgtgct ggcggttgaa aatcttaata ttgcctttat
    34921 gcaggaccag cagaaaatag ctgcggtccg caatctctct tttagtctgc aacgcggtga
    34981 gacgctggca attgttggcg aatccggctc cggtaagtca gtgactgcgc tggcattgat
    35041 gcgcttgttg gaacaggcgg gcggtttagt gcagtgcgat caaatgctgt tgcggcggcg
    35101 cagtcgtgaa gtgattgaac ttagcgagca gagcgctgca caaatgcgcc atgtgcgcgg
    35161 tgcggatatg gcgatgatat ttcaggagcc gatgacatcg ctgaacccag tatttactgt
    35221 gggtgaacag attgccgaat caattcgtct gcatcagaac gccagtcgtg aagaagcgat
    35281 ggtcgaggcg aagcggatgc tggatcaggt acgcattccg gaggcacaaa ccattctttc
    35341 acgttatccg catcaactct ctggcgggat gcgccagcga gtgatgattg cgatggcgct
    35401 gtcatgccgc ccggcggtgc tgattgccga tgagccaacc accgcgctgg atgtcactat
    35461 tcaggcgcag atcctgcaat taatcaaagt attgcaaaaa gagatgtcga tgggcgttat
    35521 ctttatcacg cacgatatgg gcgtggtggc agagattgcc gatcgggtac tggtgatgta
    35581 tcagggcgag gcggtggaaa cgggtaccgt cgaacagatt tttcatgcac cgcaacatcc
    35641 ttacacccgt gcgctgttag ctgctgttcc gcaacttggt gcgatgaaag ggttagatta
    35701 tccccgacgt ttcccattga tatcgcttga acatccagcg aaacaggccc cccccatcga
    35761 gcagaaaacg gtggtggatg gtgaacctgt tttacgggtg cgtaatctgg tcacccgttt
    35821 ccctttgcgc agcggtttgt tgaatcgcgt aacgcgggaa gtgcatgccg ttgagaaagt
    35881 cagttttgat ctctggcctg gcgaaacgct atcgctggtg ggcgagtctg gcagcggtaa
    35941 atccactacc gggcgggcgt tgctgcgcct ggtcgaatcg cagggcggcg aaattatctt
    36001 taacggtcag cgaatcgata ccttgtcacc cggcaaactt caggcattac gccgcgatat
    36061 tcagtttatt tttcaggacc cttacgcttc gctggaccca cgtcagacca tcggtgattc
    36121 gattatcgaa ccgctgcgtg tacacggttt attgccaggt aaagacgcgg ctgcacgcgt
    36181 tgcgtggttg ctggagcgcg tgggcctgtt acctgaacat gcctggcgtt acccgcatga
    36241 gttttccggc ggtcagcgcc agcgcatctg cattgctcgc gcgttggcat tgaatccaaa
    36301 agtgatcatt gccgacgaag ccgtttcggc gctggatgtt tcaattcgcg ggcagattat
    36361 caacttgttg ctcgatctcc agcgtgattt cggcattgcg tatctgttta tctcccacga
    36421 tatggctgtg gtagagcgga ttagtcatcg tgtggcggtg atgtatctcg ggcaaattgt
    36481 tgaaattggt ccacggcgcg cggtttttga aaatccgcag catccttata cgcgtaaatt
    36541 actggcggca gttccggtcg ctgaaccgtc ccgacaacga ccgcagcgtg tactgctgtc
    36601 ggacgatctt cccagcaata ttcatctgcg tggcgaagag gttgcagccg tctcgttgca
    36661 atgcgtcggg tcggggcatt acgtcgcaca accacaatca gaatacgcac tcatgcgtag
    36721 ataacattca ggcggagaat aaaatggcaa gagctgtaca ccgtagtggg ttagtggcgc
    36781 tgggcattgc gacagcgttg atggcatctt gtgcattcgc tgccaaagat gtggtggtgg
    36841 cggtaggctc gaatttcacc acgctcgatc cgtatgacgc gaatgacacg ttatctcagg
    36901 ccatagcgaa atcgttttac caggggctgt ttggtctgga taaagagatg aaactgaaaa
    36961 acgtgctggc ggagagttat accgtttccg atgacggcct tacatacacc gtgaaattgc
    37021 gggaaggcat taaattccat gatggcaccg atttcaacgc agtggcggta aaagcgaatc
    37081 tggaccgggc cagcgatccg gcgaatcatc ttaaacgcta caacctgtat aagaatattg
    37141 ccaaaacgga agcgatcgat ccgacaacgg taaagattac cctcaaacag ccgttctcag
    37201 cgtttattaa tattcttgcc catccggcga ccgcgatgat ttcaccggca gcgctggaaa
    37261 aatatggcaa ggagattggt tttcatccgg tgggaaccgg accgtatgaa ctggatacct
    37321 ggaatcagac cgattttgtg aaggtgaaaa aattcgcggg ttactggcag ccaggattgc
    37381 ccaaactgga cagcataacc tggcgtccgg tggcggataa caacacccgc gcggcaatgc
    37441 tgcaaaccgg tgaagcgcag tttgctttcc ccattcccta cgagcaggcc acactgctgg
    37501 agaaaaacaa aaatatcgag ttgatggcca gtccgtcaat tatgcaacgt tatatcagta
    37561 tgaacgtgac gcaaaagccg ttcgataacc cgaaggtccg tgaggcgctg aattacgcca
    37621 ttaaccgtcc ggcgctggtg aaagtggcct ttgcgggcta tgcaacgcca gctactggtg
    37681 tggtaccgcc gagtatcgcc tacgcgcaaa gttataaacc gtggccttac gatccagtga
    37741 aagcgcgcga attactgaaa gaggcgggat atcccaatgg tttcaatacc acgctgtggt
    37801 cgtcacataa ccacagcacc gcgcagaaag tgctgcaatt tacccagcag cagttagcgc
    37861 aggtcgggat taaagcccag gtgactgcga tggatgccgg acagcgggcg gaagaagttg
    37921 aaggtaaagg gcaaaaagag agcggcgtgc ggatgttcta cactggctgg tcggcttcaa
    37981 ccggcgaagc ggactgggca ctatcgccgc tgtttgcctc gcagaactgg ccaccgacgc
    38041 tgtttaatac cgcgttttac agcaataaac aggtggatga cttcctggct caggcactga
    38101 aaactaatga tccggcggaa aagacccgct tgtataaggc ggcgcaggat atcatctggc
    38161 aagagtcgcc gtggatcccg ctggtggtag aaaaactggt gtcggcacac agtaaaaacc
    38221 tgaccggttt ttggatcatg ccagacaccg gcttcagctt tgaagacgcg gatttgcaat
    38281 aagcaacgca gggagtggaa tgcttaatta cgttatcaaa cgcttactgg ggttgattcc
    38341 aacgctgttt atcgtctcgg tgctggtgtt tttatttgtg catatgctgc ccggcgatcc
    38401 ggcgcgattg attgccgggc ccgaagctga tgcgcaggtt atagaactgg tgcgtcagca
    38461 gctggggttg gatcagccgc tgtatcacca gttctggcac tatatcagca atgctgtgca
    38521 gggggatttt ggcctgtcga tggtgtcgcg tcgtccggtt gccgatgaga ttgccagccg
    38581 ctttatgcca acgctgtggc tgaccataac cagtatggtc tgggcggtga tatttggtat
    38641 ggcggcggga attatcgccg ccgtctggcg taatcgttgg ccggatcgat tgagtatgac
    38701 cattgcggtg tcggggatct cgtttccggc atttgctctg gggatgcttt taattcaggt
    38761 attctccgtt gaactgggct ggctgcctac cgtgggagca gacagttggc agcactacat
    38821 tttaccctcc ctgacgctcg gcgcggcagt ggccgccgtg atggcgcgct ttacccgcgc
    38881 gtcgtttgtc gacgttttaa gcgaagatta tatgcgtacc gcgagggcga aaggggggag
    38941 cgaaacctgg gttgtcctca aacacgggct acgcaacgcg atgatcccgg tagtgaccat
    39001 gatgggctta cagtttggct ttttgctcgg tggttccatc gtggtggaga aagttttcaa
    39061 ctggcccgga cttggacgct tactcgttga ctccgtagaa atgcgcgatt acccggtgat
    39121 tcaggcggaa attctgcttt tctcgctgga atttattctt atcaacttag tggtggatgt
    39181 gctttacgcc gccattaacc cggctatcag gtacaagtaa ggatgcgact atttaactgg
    39241 cgacgtcagg cggtgttaaa cgccatgcca ctggtcaaac ctgaccaggt acgtacaccg
    39301 tggcatgaat tctggcgacg atttcgccgt cagcatatgg cgatgaccgc cgcattgttc
    39361 gttattttat tgattgtggt ggctattttt gcacgctgga tcgctcccta tgacgccgaa
    39421 aattattttg attatgacaa tctgaataac ggaccttctt tgcagcactg gtttggcgtc
    39481 gattcactgg ggcgtgacat tttcagccgt gtcctggttg gtgcgcaaat ctcgctggcg
    39541 gcgggcgtgt ttgccgtgtt tatcggtgcg gcgatcggga cgttgctggg cttgttggct
    39601 ggatattatg aaggctggtg ggatcggctg atcatgcgca tttgcgatgt gctgtttgcc
    39661 ttcccgggta ttttactggc gatcgctgtt gttgcggtgt tgggaagcgg cattgctaac
    39721 gtgattatcg cagtcgccat tttttccatc cccgcgtttg cccgactggt gcgcggcaac
    39781 acgctggtgt tgaaacagca aacctttatt gagtcagcac gcagtattgg tgccagcgat
    39841 atgaccattt tattgcgtca tatcttgcct gggaccgtct cttctatcgt ggtgtttttc
    39901 accatgcgca ttggtacctc gattatctct gccgccagcc tgtcatttct cggcctcggt
    39961 gcgcagccgc cgacaccaga gtggggagca atgctcaatg aggctcgagc ggatatggtt
    40021 atcgcgccgc atgtcgctgt ttttccggcc ctggctattt ttctgactgt actggcgttc
    40081 aatttgttgg gcgatggttt acgcgatgcg ctggatccga aaattaaagg atagttacgt
    40141 ttgaatattg cttgaaagag taatcacctc acaggaaatt attgccctaa gcaagtgttg
    40201 taactttctg ctgattttgt agaatcgggt aatttggtta aatagccgca gcaagggact
    40261 gtttttgcag cggcacagcg ttcagacagt tattttgtta aatgtattaa catgctgagt
    40321 ttatacgaaa agataaagat aaggctgata attttatttt tattggcagc actgtcattt
    40381 attggccttt ttttcatcat taactatcaa ctggtatcgg aacgcgcggt aaaacgtgcc
    40441 gatagccgct ttgaacttat tcagaaaaac gttggctatt tctttaaaga tattgaacgt
    40501 tcggccctga cattaaagga ctcactgtat ttattaaaaa atacagagga gattcaacgc
    40561 gctgtgatcc tgaaaatgga aatgatgcca tttttagact cggtgggact ggtacttgat
    40621 gataataaat attatctctt ttcgcggagg gcgaacgata aaatcgttgt ttatcatcag
    40681 gaacaagtaa atggaccgct tgtcgacgag tcagggcggg ttatttttgc cgattttaac
    40741 ccatcgaaac gaccgtggtc ggtggcttca gatgactcta acaacagctg gaatccggca
    40801 tacaattgct ttgatcgtcc gggtaaaaaa tgtatctctt ttacgctacg catcaacggc
    40861 aaagatcacg atttgttagc ggtggataaa attcatgtcg atttaaactg gcgatatctg
    40921 aacgagtatc ttgatcaaat cagcgctaat gatgaagttc tatttttgaa acaaggccat
    40981 gagatcattg ccaagaatca actcgctcgt gaaaaactga ttatttataa tagcgaag
//
LOCUS       DAZFFG010000026        40392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFFG010000026 DAZFFG010000000
VERSION     DAZFFG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.026"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            129..218
                     /locus_tag="ACHJ7J_001778"
                     /pseudo
     CDS             129..218
                     /locus_tag="ACHJ7J_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            246..989
                     /gene="yciC"
                     /locus_tag="ACHJ7J_001779"
     CDS             246..989
                     /gene="yciC"
                     /locus_tag="ACHJ7J_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO5000024.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            1019..1558
                     /gene="yciB"
                     /locus_tag="ACHJ7J_001780"
     CDS             1019..1558
                     /gene="yciB"
                     /locus_tag="ACHJ7J_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO5000025.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            1663..2061
                     /gene="yciA"
                     /locus_tag="ACHJ7J_001781"
     CDS             1663..2061
                     /gene="yciA"
                     /locus_tag="ACHJ7J_001781"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO5000026.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(2101..2820)
                     /gene="tonB"
                     /locus_tag="ACHJ7J_001782"
     CDS             complement(2101..2820)
                     /gene="tonB"
                     /locus_tag="ACHJ7J_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO5000027.1"
                     /translation="MTFDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            3044..3340
                     /gene="yciI"
                     /locus_tag="ACHJ7J_001783"
     CDS             3044..3340
                     /gene="yciI"
                     /locus_tag="ACHJ7J_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO5000028.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            3640..4893
                     /gene="kch"
                     /locus_tag="ACHJ7J_001784"
     CDS             3640..4893
                     /gene="kch"
                     /locus_tag="ACHJ7J_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO5000029.1"
                     /translation="MSHWATFKQTATNLWVILRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(4947..5120)
                     /gene="yciY"
                     /locus_tag="ACHJ7J_001785"
     CDS             complement(4947..5120)
                     /gene="yciY"
                     /locus_tag="ACHJ7J_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO5000030.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            5263..6723
                     /gene="cls"
                     /locus_tag="ACHJ7J_001786"
     CDS             5263..6723
                     /gene="cls"
                     /locus_tag="ACHJ7J_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO5000031.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            6758..7087
                     /gene="yciU"
                     /locus_tag="ACHJ7J_001787"
     CDS             6758..7087
                     /gene="yciU"
                     /locus_tag="ACHJ7J_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO5000032.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKI
                     CHIIWRE"
     gene            complement(7140..8144)
                     /gene="oppF"
                     /locus_tag="ACHJ7J_001788"
     CDS             complement(7140..8144)
                     /gene="oppF"
                     /locus_tag="ACHJ7J_001788"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO5000033.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(8141..9154)
                     /gene="oppD"
                     /locus_tag="ACHJ7J_001789"
     CDS             complement(8141..9154)
                     /gene="oppD"
                     /locus_tag="ACHJ7J_001789"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO5000034.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(9166..10074)
                     /gene="oppC"
                     /locus_tag="ACHJ7J_001790"
     CDS             complement(9166..10074)
                     /gene="oppC"
                     /locus_tag="ACHJ7J_001790"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO5000035.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVIVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(10089..11009)
                     /gene="oppB"
                     /locus_tag="ACHJ7J_001791"
     CDS             complement(10089..11009)
                     /gene="oppB"
                     /locus_tag="ACHJ7J_001791"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO5000036.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(11095..12726)
                     /gene="oppA"
                     /locus_tag="ACHJ7J_001792"
     CDS             complement(11095..12726)
                     /gene="oppA"
                     /locus_tag="ACHJ7J_001792"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO5000037.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(13464..14111)
                     /gene="ychE"
                     /locus_tag="ACHJ7J_001793"
     CDS             complement(13464..14111)
                     /gene="ychE"
                     /locus_tag="ACHJ7J_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO5000038.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(14142..14195)
                     /gene="ychE"
                     /locus_tag="ACHJ7J_001794"
     CDS             complement(14142..14195)
                     /gene="ychE"
                     /locus_tag="ACHJ7J_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO5000039.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            14588..17263
                     /gene="adhE"
                     /locus_tag="ACHJ7J_001795"
                     /gene_synonym="adhC"
     CDS             14588..17263
                     /gene="adhE"
                     /locus_tag="ACHJ7J_001795"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO5000040.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(17564..18181)
                     /gene="tdk"
                     /locus_tag="ACHJ7J_001796"
     CDS             complement(17564..18181)
                     /gene="tdk"
                     /locus_tag="ACHJ7J_001796"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO5000041.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            18785..19198
                     /gene="hns"
                     /locus_tag="ACHJ7J_001797"
     CDS             18785..19198
                     /gene="hns"
                     /locus_tag="ACHJ7J_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO5000042.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(19342..20250)
                     /gene="galU"
                     /locus_tag="ACHJ7J_001798"
     CDS             complement(19342..20250)
                     /gene="galU"
                     /locus_tag="ACHJ7J_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO5000043.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(20452..21465)
                     /gene="rssB"
                     /locus_tag="ACHJ7J_001799"
     CDS             complement(20452..21465)
                     /gene="rssB"
                     /locus_tag="ACHJ7J_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO5000044.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(21557..22462)
                     /gene="rssA"
                     /locus_tag="ACHJ7J_001800"
     CDS             complement(21557..22462)
                     /gene="rssA"
                     /locus_tag="ACHJ7J_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO5000045.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            22575..23033
                     /gene="ychJ"
                     /locus_tag="ACHJ7J_001801"
     CDS             22575..23033
                     /gene="ychJ"
                     /locus_tag="ACHJ7J_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO5000046.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            23083..23925
                     /gene="purU"
                     /locus_tag="ACHJ7J_001802"
     CDS             23083..23925
                     /gene="purU"
                     /locus_tag="ACHJ7J_001802"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO5000047.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            24085..24169
                     /locus_tag="ACHJ7J_001803"
     tRNA            24085..24169
                     /locus_tag="ACHJ7J_001803"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24119..24121,aa:Tyr,seq:gta)
     gene            24217..24348
                     /locus_tag="ACHJ7J_001804"
     ncRNA           24217..24348
                     /ncRNA_class="other"
                     /locus_tag="ACHJ7J_001804"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(24532..25209)
                     /gene="narI"
                     /locus_tag="ACHJ7J_001805"
     CDS             complement(24532..25209)
                     /gene="narI"
                     /locus_tag="ACHJ7J_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5000048.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(25209..25919)
                     /gene="narJ"
                     /locus_tag="ACHJ7J_001806"
     CDS             complement(25209..25919)
                     /gene="narJ"
                     /locus_tag="ACHJ7J_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5000049.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(25916..27454)
                     /gene="narH"
                     /locus_tag="ACHJ7J_001807"
     CDS             complement(25916..27454)
                     /gene="narH"
                     /locus_tag="ACHJ7J_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5000050.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(27451..31194)
                     /gene="narG"
                     /locus_tag="ACHJ7J_001808"
     CDS             complement(27451..31194)
                     /gene="narG"
                     /locus_tag="ACHJ7J_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO5000051.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TADELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(31587..32978)
                     /gene="narK"
                     /locus_tag="ACHJ7J_001809"
     CDS             complement(31587..32978)
                     /gene="narK"
                     /locus_tag="ACHJ7J_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO5000052.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            33317..35113
                     /gene="narX"
                     /locus_tag="ACHJ7J_001810"
     CDS             33317..35113
                     /gene="narX"
                     /locus_tag="ACHJ7J_001810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO5000053.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            35106..35756
                     /gene="narL"
                     /locus_tag="ACHJ7J_001811"
     CDS             35106..35756
                     /gene="narL"
                     /locus_tag="ACHJ7J_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO5000054.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(35757..37151)
                     /gene="ychO"
                     /locus_tag="ACHJ7J_001812"
                     /gene_synonym="iatA"
     CDS             complement(35757..37151)
                     /gene="ychO"
                     /locus_tag="ACHJ7J_001812"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO5000055.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            37337..37690
                     /gene="ychN"
                     /locus_tag="ACHJ7J_001813"
     CDS             37337..37690
                     /gene="ychN"
                     /locus_tag="ACHJ7J_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO5000056.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(37734..38429)
                     /gene="chaC"
                     /locus_tag="ACHJ7J_001814"
     CDS             complement(37734..38429)
                     /gene="chaC"
                     /locus_tag="ACHJ7J_001814"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO5000057.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(38587..38817)
                     /gene="chaB"
                     /locus_tag="ACHJ7J_001815"
     CDS             complement(38587..38817)
                     /gene="chaB"
                     /locus_tag="ACHJ7J_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO5000058.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            39087..40187
                     /gene="chaA"
                     /locus_tag="ACHJ7J_001816"
     CDS             39087..40187
                     /gene="chaA"
                     /locus_tag="ACHJ7J_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO5000059.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATCDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT        10196 a        10293 c         9639 g        10264 t
ORIGIN      
        1 gattaattta gaagttgcat tgagaatcct ggttaacgtg atttaaatca atttttaaat
       61 attttcgcta cgcaaatatt aactcgttcg ccacttgccg cggtttgccc acgcttgaag
      121 gccagcaatt acctccgctt tacaaagata tgcttttcca tacaagacct gatgctgcca
      181 cccgaggctt aaccagggta caattgcccg ctaattaaca cctgcataaa actcaaggag
      241 agtgcatgtc tatcacggcg cagtccgtat accgtgacac cggaaatttc ttccgtaatc
      301 aatttatgac cattctgttg gtatcgttgc tatgtgcgtt tatcacagtg gtgttagggc
      361 atgttttctc acccagtgat gcacagcttg cgcagctcaa tgacggcgtg cccgttagcg
      421 gcagtagtgg gttgttcgac ctggttcaga atatgtcacc ggaacagcaa caaattttgc
      481 tgcaggcttc agcggcgtcc actttttcag gattaatcgg taacgccatt ctcgccggag
      541 gcgtaatatt aattatccag ctggtgtctg cgggtcagag agtcagtgcg ctgcgagcta
      601 ttggtgccag tgcgccgata ttgccaaagt tatttattct gatttttctg actacccttt
      661 tagtacagat tggcatcatg ctggtggtcg ttccgggaat tatcatggcc attttactgg
      721 ccctggcacc ggtgatgttg gtacaggaca aaatgggcgt ttttgcctcg atgcgtagca
      781 gtatgcggct gacttgggcg aatatgcgtc tggtggcacc cgcagtactg agctggttgc
      841 tggcaaaaac actgttgctg ctttttgcct cttcttttgc cgcattaacc ccggaaattg
      901 gtgccgtact ggcgaacacc ttgagcaacc tgatttcagc cgtattgctc atctatctgt
      961 tccgtctgta tatgttgatt cgccaataac cttaagcatt tgattacgga atcgtaaaat
     1021 gaagcagttt cttgattttt taccgctggt tgtctttttc gcgttttaca agatttatga
     1081 tatctatgcg gctactgcgg cgctgatcgt cgccacggcg attgtgctta tctatagctg
     1141 ggttcgcttt cgtaaggttg agaagatggc cctgatcact tttgttctgg tggtcgtctt
     1201 cggtggcttg acgctgttct tccacaatga tgagtttatt aaatggaagg ttacagtcat
     1261 ttatgctctg tttgcgggag ccctgttagt cagccaatgg gtgatgaaaa agccgctaat
     1321 tcagcggatg ctgggtaaag aactcacgct gccgcaaccg gtatggtcga agctgaatct
     1381 ggcctgggct gttttcttta tcctttgcgg tctggcaaac atctacatcg cattctggct
     1441 gccgcaaaat atttgggtca actttaaagt ctttggcctg accgccctta ccttaatctt
     1501 cacattgtta agcggtatct atatctaccg ccacatgccg caggaagata aatcctaacc
     1561 agactgccag tccttcgggg ctggcgctgt atctcctctg ttctcctcaa gaaaatcata
     1621 gtagcatcgc gcctgtgatt ttccttttaa gtcggtttta ccatgtctac aacacataac
     1681 gtccctcagg gcgatcttgt tttacgtact ttagccatgc ccgccgatac caatgccaat
     1741 ggtgacatct ttggtggttg gttaatgtca caaatggata ttggcggcgc tattctggcg
     1801 aaagaaattg cccacggtcg cgtagtgacc gtgcgggttg aaggaatgac tttcttacgg
     1861 ccggttgcgg tcggcgatgt ggtgtgctgc tatgcacgct gtgtccagaa agggacgaca
     1921 tcggtcagca ttaatattga agtgtgggtg aaaaaagtag cgtctgaacc aatcgggcaa
     1981 cgctataaag cgacagaagc attatttaag tatgtcgcgg ttgatcctga aggaaaacct
     2041 cgcgccttac ctgttgagta atagtcaaaa gcctccggtc ggaggctttt gactttatgc
     2101 ttactgaatt tcggtggtgc cgttaatttt aaacaggatg ttcaccacaa tcccactgcc
     2161 tggcttaccc ggctcataac gccatctgcg catcgcattt ttcacctcac gctcaaacat
     2221 gttcgcaggc ttggctgaga ggatttgtac gttatccacg cgaccatccg gcgtgacatc
     2281 aaatttaact ttaacctgcc cttcaatgcg caatgcctgt gcccgtgccg gatactgcgg
     2341 ctgattacgg cttaatgcgc gtggtcctga agccacactg gtaaccggct tgctggttgc
     2401 agccgttgct gtacttgatg tcgggcgtgc cggtgccgta ttttcaaacg gtgatgccgg
     2461 acgcgactct acgggtttga catcgcgttt tggctgttcc tgtacctttt tcaccggctt
     2521 cggttttggc ttaggtttcg gcttcggctt ttcaatgacc accggagctt cttttggcgg
     2581 ttcggggatc ggctcaggtt ccggttctgg ctctaccacc ggctccggtg gcggctgaac
     2641 ggcttgtggc ggttcgagat cagcaggcgc aaccatcgtg acagaaatcg gctgcgcagg
     2701 cgcaggtagt tcaataacct gatgtaccga ggtatagagc agacccgcca caacagcacc
     2761 atgaatgcag accgaaagta acgtcggcca ggggaagcgg cgaggtaaat caaaggtcat
     2821 tgaagtcata atcatttcag tagaaaaacc aggcctcgat tttaaatgca aatagcaatc
     2881 atattcaata aggcaagacg atcaggatca gtgaaaatgt catttttggc gtaattgacc
     2941 ttactttatg catggtctta acaaagacat catctttccg ttgcagatct tcgccctttc
     3001 aaataacgta ctttacaact ttcccgaaca aggagttgtg cccgtgttgt atgttattta
     3061 cgctcaagac aaagctgata gcctcgaaaa acgcctttcc gttcgtccgg cacatttagc
     3121 acgtttacag ttactgcatg atgaaggtcg tttgttgaca gctggtccaa tgccagcggt
     3181 agacagtaac gatccagggg ctgcgggatt tactggctca accgttattg ctgaatttga
     3241 atcgctggaa gcagctcagg cctgggccga tgctgacccg tacgtagcgg caggtgtcta
     3301 tgaacacgtt tcagtgaaac catttaagaa agtgttctga atagagattc aatatttaag
     3361 ggcacggttt ttgcaaccgt gttcttgttt ggcctttcct tatagccggt cagtgcattc
     3421 accaccacaa gcagataata actttatctg tatttcttta tatgatgtga gcatattcag
     3481 gaaaaatgac ctttcctgag gtacagttgc tcgctcaaaa cctttgatta ctgcggataa
     3541 agtcatctac tattgtttaa cacattcacg cgctgcatgt atgttcagaa aatgtgcacg
     3601 ctaagataat taaacaaaat tgtggttaca gaggtatcag tgagtcactg ggctacattc
     3661 aaacaaacag caacaaattt atgggtgatc ctacggcacg acattctcgc gctggccgtc
     3721 tttttaaatg gattgcttat ttttaaaaca atctatggta tgtcggtcaa tttgcttgat
     3781 attttccata tcaaagcatt ttcagagttg gatctctcct tgctggcaaa cgccccacta
     3841 tttatgctcg gcgtctttct tgtcctgaac tccattggct tactgttccg ggcaaagctc
     3901 gcatgggcaa tcagtatcat tttgttgttg atagcgctaa tttacaccct gcatttttat
     3961 ccctggctaa aatttagtat tggattttgc atttttacgc tggtgttttt gctgatactg
     4021 cgcaaagact tctcccacag tagcgccgca gccgggacaa tttttgcatt tattagtttc
     4081 acgacattac tgttttactc aacctacggt gcgctttatt taagcgaagg ttttaatccg
     4141 cgaatagaaa gtttgatgac cgcgttctat ttttcgatag aaaccatgtc aaccgtcggc
     4201 tatggcgata ttgtccctgt ttctgaatca gcacgattgt tcactatttc ggtcattatt
     4261 tccggcatta ccgtttttgc cacatctatg acctcaattt ttggtccgct tatccgcggg
     4321 ggattcaaca aacttgtaaa aggaaacaat catacaatgc atcgtaaaga tcattttatt
     4381 gtttgcggac attcgattct cgccatcaat acgatcctgc aactgaatca acgcggacaa
     4441 aacgtaacgg ttatcagcaa cttgcctgaa gatgatatca agcaacttga gcaacgctta
     4501 ggcgataacg cagatgttat ccccggtgac agtaatgaca gttctgtatt aaagaaagcg
     4561 ggaatcgatc gttgccgggc cattctggcg ctgagtgata acgatgcaga taacgcgttt
     4621 gttgtactct cggcaaaaga tatgagcagt gatgtcaaaa cagttctcgc cgtcagtgat
     4681 agcaaaaacc tgaataagat taagatggta catccggata tcattctctc accgcaactg
     4741 tttggcagcg aaattctggc gcgagtttta aatggtgaag agattaataa tgatatgctc
     4801 gtttcaatgt tgttgaactc cggtcatggc attttcagcg ataacgatga acaagaaacg
     4861 aaagctgaca gtaaagaatc ggtgcaaaaa tagagttaaa gacggaaaag taaacttatt
     4921 gggcaccgca atggtgccca gaacatttag atattgtaga tcgcaaacaa taacgagtta
     4981 cgctgtttgt ttagaatgca cttcctgatg ctgtggatac gcatatttcg gcgcgattgc
     5041 ccctcaagcc aacgcgattt acgtcggcta gcctgacgct gcatgcgcca gcgccccact
     5101 tccgttctac tccgcttcat gttaactact ctatgcaata acagaatggt cattataccc
     5161 ccgcccccga cgctgaccag tgcttttccc gcgtttttat ttgccagatg aatccatatg
     5221 cgtaaactca taacaatgcg ctttcaaaag gatttctaat ctatgacaac cgtttatacg
     5281 ttggtgagtt ggttggccat tctgggatac tggttgctca ttgcaggcgt aactttacgc
     5341 attctaatga aacgacgcgc agttccctcc gcgatggcct ggctgttgat tatttacatt
     5401 ctgccgttag tcggaattat tgcctatctt gccgttggcg agctccattt aggcaaacgc
     5461 cgcgctgagc gcgccagagc gatgtggcct tccaccgcaa aatggcttaa cgaccttaaa
     5521 gcctgtaagc atatcttcgc cgaagaaaat agcagtgtcg ctgcgccatt attcaagctt
     5581 tgcgagcgtc gtcaggggat cgctggggtc aaagggaatc agctacaatt gatgaccgag
     5641 tcagatgatg tgatgcaggc gttaatccgc gacatccagc tcgcgcgcca taatattgag
     5701 atggtgtttt atatctggca gcccggcggc atggcggacc aggtggctga atcattaatg
     5761 gcggctgcgc gacgcggcat tcattgccga ttgatgctcg actccgccgg gagtgtggct
     5821 tttttccgca gcccgtggcc cgagctaatg cgtaatgccg gtattgaagt ggtcgaagcc
     5881 ttaaaggtca atctgatgcg tgtgttttta cgccgtatgg acctgcgcca acatcgcaag
     5941 atgatcatga tcgataatta catcgcgtac accggcagca tgaatatggt cgatcctcgc
     6001 tacttcaaac aagatgcggg cgtagggcaa tggattgatc tgatggcgcg tatggaaggc
     6061 cccatcgcca ccgcgatggg gattatttat tcctgcgact gggagattga aacagggaaa
     6121 cgtattctac cgccaccacc tgatgtcaat attatgccgt ttgagcaggc cagcggtcat
     6181 accattcaca caattgcttc tggccccggc tttccggaag atctcattca ccaggcatta
     6241 ttgactgcgg cttattcggc gcgtgaatat ttgatcatga ccacgcccta ctttgtgcca
     6301 agcgatgatt tacttcatgc gatttgcacg gcggcgcagc gcggggtgga tgtcagtatt
     6361 atccttccgc gaaaaaatga ctcgatgctg gtcggctggg ccagtcgcgc attctttaca
     6421 gaactgcttg ctgctggggt taaaatttat cagtttgaag gcgggttact gcataccaag
     6481 agcgtgctgg tcgatggcga actaagtctg gttggcaccg ttaaccttga tatgcgtagt
     6541 ctgtggctga atttcgagat taccctggca atcgacgata aaggttttgg tgcagacctt
     6601 gccgccgttc aggacgatta tatttcgcgt tcacgtctgc tcgatgcccg tttatggcta
     6661 aaacgtccat tatggcaacg tgtcgccgag cgactgtttt acttcttcag cccgttgctg
     6721 taaaacgtcg ccaacagagg ttaaacaggt agtgactatg gatatggatc taaacaatcg
     6781 cctgactgaa gatgaaacgc ttgagcaggc ctacgatatt tttctcgaac tggctgctga
     6841 caatctcgat ccagctgatg ttctgctgtt caatcttcag tttgaagagc gcggcggcgc
     6901 ggagttattc gatccggcgg aagactggca ggaacatgtt gatttcgatt tgaacccgga
     6961 cttttttgcc gaagtggtga ttggtctggc ggacagtgaa gatggtgaaa tcaacgatgt
     7021 tttcgcccgc attctgttat gccgcgaaaa agatcacaaa atttgccata tcatctggcg
     7081 ggaataaata atcgctttta gcgtgaaaaa gggctgacaa ctgtcagccc ttattgtttt
     7141 taaagcggat cgactttcag gcaagaaacg gcgtgtctga aactcccctc cagaacagga
     7201 cgtgttttgg cgcactctgg accggcaatc gggcaacggg tacggaaaac acaaccggaa
     7261 ggcgggttga tcggcgacgg taattcccct tccagtaact ggatggtttt gttcttctcc
     7321 agatccggat caggtatggg gactgccgac atcaatgccc tggtgtaagg atgtagtgga
     7381 ttgtggtaga cctcatcata ggtccccagt tctaccgcat ggccgagata catcaccaac
     7441 acacgatcgg aaatgtgttt taccacggcc aggtcgtgag cgataaaaat taatgacaat
     7501 cccatctcac gttgtaactg ctggagcagg ttgaccacct gcgcctgaat tgacacgtcc
     7561 agcgcagaca ccggctcatc acagataatc agcttcggtt caagaataag agcacgagca
     7621 atcccgatac gctggcactg cccaccggag aactcatgcg gatagcggtt aatcaggtta
     7681 ggcaataacc cgactttcag catcatcgcc ttcacgcgct cgcgaacttc ctggcgtgac
     7741 attttcggat gataggtacg cagcggttca gcgataatct cgccgatggt catacgcggg
     7801 tttagcgatg ccaacggatc ctggaaaatc atctgaatat cactgcgaac ggcacgccat
     7861 tcatcgggct tcatgcccag caactcttta cctaaccagg caacatgacc gtcggtcgcc
     7921 ttgaccaaac cgatgatggc gcgagcaaag gtggacttac cgcatcccga ttcccctacc
     7981 acacctaatg tttccccttc atacaggcga agagttacac catctacggc tttgagagtt
     8041 ttcggtggtt gccagaacca ctgtttgcca tctttgattt caaagtgcac tttcagatcg
     8101 gcgatttcga ggaggacttt tcttccttcg gttacagcat tcataacagt tcctccaccg
     8161 gtttaaagca agcacgcaga cggccaggcg taaactcttc cagcggcggt gcgctactac
     8221 aaatttccat cgcatgcgga caacgtggct ggaacgggca acctttcggt aatcgcagca
     8281 ggtttggcgg attacccgga atagtcaaca ttgtttcacc ttccgcatcg agacgaggca
     8341 ccgcgttgag caaaccgata gaataaggat gaacgggttg ataaaagaca tcgcgcgcgt
     8401 tgccatattc catcgtgcgc ccggcgtaca ttaccagcac tttgtcgcag atccccgcca
     8461 ccacgccaag atcgtgggtg atcataatga tggcggtatt aaattcccgc ttcagttcat
     8521 tcaatagcgt catgatctgc gcctgtacgg tgacgtccag cgcagtagtg ggttcatccg
     8581 caatcagcag cttaggtcga catagcaatg ccatcgcaat cataactcgc tgacgcatgc
     8641 cgccagaaaa ttcgtgcggg tacattttca tgcgtttacg tgcttccggc atttttaccg
     8701 catcgagcat ccgcaccgac tcttcaaacg cctcagcttt gctcatgttc ttatgcagca
     8761 tcagcacttc catcaactgc tcaccgacgc gcatataggg attcaacgaa gtcattgggt
     8821 cctgaaaaat cattgagatt tgttcagcac gcagtttatt gagttcacgc tctggcaaat
     8881 tgaggatttc acgcccattg aaggttgccg atccgccaat acgtccgttg gcagccaaca
     8941 ggcccatcaa cgcaaatgca gtttgcgatt tacccgaacc agactcacct acaatgccca
     9001 gcgtttctcc ggcacgtagg gaaaaattca aatcattgac cgccgtgacg tcgccgtccg
     9061 gggtactaaa ggtgacacgc aaatctttca cgttcagcag tgcgtcagcc tgttgttgtg
     9121 cgagcggcac agttgcagtt tcaattacgc tcatggctgc actccttaac gatctttcgg
     9181 gtcgagggca tcacgcaagc catcgccgat aaagttgaaa caaaacagcg tcaccacgag
     9241 gaatcccgct gggaacaaca gtaaccatgg agagacttcc atcgagttcg cgccatcact
     9301 cagcaatgcc ccccagctgc ttaacggctc ttgcgtaccc aaccccagga agctaaggaa
     9361 agattcaaag aggatcatgc tgggcaccag tagcgatgcg tagaccacca ccacaccgag
     9421 tacgttcggc acaatgtggc gaataacaat gcccggcgtc gatacaccgc caacttgtgc
     9481 cgcctcaata aactctttgc gcttcagact cagggtttgt ccacgcacaa tacgagccat
     9541 atccagccag gaaaccatac caatcgccac gaaaatcagc aggatgtttt gaccgaaaaa
     9601 ggtcaccagc aaaatgacga agaacatgaa tgggaaggag ttgaggattt ccagcagacg
     9661 catcattacc gaatccactt taccgcccag ataaccggaa agcgaaccat aaagtgtccc
     9721 cacgatcact gccaccagtg ccgcagcaac accgaccatg agtgagatac gcccgccaat
     9781 cgcaacgcgc acaagcaggt cgcgaccgga tgagtcagta ccaaagtagt gaccggactc
     9841 catatccggg gcgctggaca tcatcgccca gtcagtatcg tcataggcaa actgcgaaag
     9901 catcggtgcc aggattacaa ataacgcgat cagcaccagc actatcagac tggcaaccgc
     9961 cgcacggtta tgcataaaac gtcgacgtgc gtcctgccac aagctgcgcc cttcgacctc
    10021 cagcttttca ctgaaatttt ccagcgtctc gctgtttttc ttacttaaca tcatcgcgag
    10081 ctccagtatc agtaacggat tttcgggtcg ataaccgcat atagcacatc gacaatggca
    10141 ttaaacaaaa tggttaacgc accaaccagg atggtcaggc ttaacactaa ggaatagtca
    10201 cggttcaatg cgccattaac gaacagttgc ccaatccccg gcaaaccata aatggtttcg
    10261 ataaccatag aaccggtaat aatgccgaca aatgcggggc ccatatagga gagcacgggt
    10321 aacagagcag gttttaatgc gtggcgtaaa atgatccgcc gcataggtaa ccctttcgcc
    10381 cgggcagtac gaataaagtt ggagtgtaat acttcaatca tagagccacg ggtaatacgc
    10441 gcaatactgg cgatataggc gagtgacaac gccaccatcg gcaatatcat gaatttaagc
    10501 gccccaccat tccagccacc gcccggcagc caatgcaaaa tgatcgcaaa tatcatgact
    10561 aataatggcg caaccacaaa actggggata acaaccccgg tcattgccag ccccatcacg
    10621 gtatagtccc atttggtgtt ttgttttaat gcggcaataa cgccagcact aacacccagt
    10681 attaccgcaa ggaaaaatgc tgcggctccc agtttggcag aaacgggaaa actggatgca
    10741 accaggtcat tgaccgaata atctttatat ttaaacgaag gaccgaaatc gccatgcgcc
    10801 agttgtttca ggtagctgaa atactgtgtc atgatcggat cattaagatg atatttcgct
    10861 tcgatattgg ccattacttc cggtggtaaa gtacgttcgc cggtaaaagg gcttcccggc
    10921 gcgaggcgca tcataaagaa cgaaatagta ataagaataa atagcgtcgg aatcgcttcc
    10981 agacagcgac gtagaataaa ttttaacatt gccctacctt ctggcctgtg ccttctgtaa
    11041 tgcgataaaa atcagacacc gtggagcagg acactcctgc cccacgtatt gccattagtg
    11101 cttcacaatg tacatatttc gggtgtaggt attatccagc ggatctttgc cggtataacc
    11161 accaacccac ggtttcacca gacgcgcatt cacgtagtaa taaacaggaa caatggccga
    11221 atccttatcc agctgttgtt ctgctttagt gtacagagct gtgcgctgcg cctcgtcagt
    11281 cactttcagc gtttccgcca taatgctgtc aaaggccggg ctcttataat gcgcggtatt
    11341 catcgagctg ttcgaaagca tggtgttgag gaaggaagtt ggttcgttgt agtcagcaca
    11401 ccagcctgca cgggccacat caaaagtacc ctggtgacgg gtgtcgagga acgttttcca
    11461 ctcctggtta accagtttga cgtttacacc aatgtttttc ttccacaatg aagaggcagc
    11521 aatcgccagc tttttatgca gatcggaggt gttatacagc aggttgatgg tcaacggttt
    11581 gtctgcggta taacccgctt cagccagcag ttttttcgct tcttcgttac gtttttcctg
    11641 gctccagcca aaccattctg gctgagtcaa ttttgcgcca tcagtatacg gtggagtgta
    11701 accataggcg ggcatgtcgc cctgcgcttt cactttatta acaatgatat cgcggtccat
    11761 acctagtttc agtgcggtac gcacacgcac atcgttgaat ggcggtttct ggttgttaat
    11821 ttcgtaatag taagtgcaca ggtatggatc aacgtgaact tcgtccggga tctctttttt
    11881 cagcttctgg aacaattcga tcggcatgtt gttataagtc atgtcgattt caccactacg
    11941 gtagcggttg acatcggtaa cttcagaagc aataggcaaa taggttacct ggttaataac
    12001 ggttttcgcg ttgttccagt aggttgggct gcgttcaaga acgattcgtt cgtttacgac
    12061 ccaatctttt aaggtatagg caccgttggt gacgatatta ccaggctggg tccatttttc
    12121 gccgaatttc tcgatagcgg cttttggcac cggtgaagtt gatgggtgaa caagtaattt
    12181 atagaagtac ggaacgggtt cacttaaggt gacttctaat gtgtgatcat caatagcttt
    12241 cacgccgaga tcggtaatcg gttttttccc ttcaagaatt tcatcaatac cggcgatatg
    12301 cccatattgc agataactgg catacggaga agcagtgttc ggatcaacag aacgttgcca
    12361 gctatacaca aagtcttgtg ctgtgactgg cgtgccatca gaccatttcg catctttacg
    12421 caaatggaag gtccagactt tcgcgtcttt attatcccag gattcagcga cgccaggtgc
    12481 cggatggccg tcaagatcgc tgaccagtaa gccttcaaac aggtctcggc tgatattaga
    12541 ctccggaaca ccttcaattt tgtgcggatc taatgactga acttctgaac cattgttacg
    12601 taccagcgtt tgtttttccg ccagtgtgac gcctgcgggt acatcagctg ccagcgcgac
    12661 attccctgcc attagcgcag ccagaacgcc agctgctact aaacttctct tggtgatgtt
    12721 ggtcattgtt ttttggactc cctcattata attactggta ttaaccagca tgtgtaatcc
    12781 cggaatggga ccctgcacag gctcggagac tttctgctgt caggttactt tatcgttacg
    12841 gattgctatc accgacttta tttattctgg tgattcagac gatcacctta tgtcgattcg
    12901 tactgtgtct cccctgtcga gacacattat tctttcgcga tgctctttaa ttgttaatta
    12961 ttctcattac caataaagaa tcgcctggcg gttgctcgga aagtaccaaa tgcgcttctt
    13021 actcgccaat acattttgca aaattgttaa gcaattctct tttattgacc gtatcagaat
    13081 ctatagcttg catccatgcc ttaacaaata tctttaaatt caatcaaata gagattggtt
    13141 aagcatgtct atcttttctg attacagttc cagtagtgaa atgcacaata atttaacaat
    13201 tgattattat ttagcacttt cctctaccaa aggcagtgga attactaata tcatttcaat
    13261 tattcttcag caagcccagg attatgatgt ggcgaaaata acatgatggt gaggagaagc
    13321 agttaaggaa atccttataa ctaactgtaa tttgagaaga atatttttac ctattccttt
    13381 tgcttttgat aactgcgcgg gaacaaagta aaaataatga cgaagtaaac ggagcatggc
    13441 agctccgttt catttgaaag gaattaatta agcaggccgg ggaaaatccc cttaatacca
    13501 gtaacgataa attcaatccc caatgccatc agcaatagcc ccataatacg cgtaatcacg
    13561 ttgatgccgg tctggcgtaa aacccgtacc agccacggtg ccatgcggaa caatccccag
    13621 caacataaag cgaacaatgc aatagccaca aagaaaccaa acagatagct aatgctgtga
    13681 taacgcgtac cccagacgat ggtagaactg atcgcccctg gccccgccat caacggcaac
    13741 gccagtggca ccacaccaat actttcacgt accgcggttt ctgatttttc ttgcttgttc
    13801 tgtttatcct cgccaagctt gccgctgatc atcgacatcg ctatcgtcac caccaggatg
    13861 cccccggcga tacggaacga atcaattgat ataccaaaaa gttgcagaat cgtgtcgccg
    13921 agaaaaagcg agatccacaa gataatggcc acagacaggt tggctgtaag gttagttttg
    13981 tttcgcgctg ccgctgtctg ataactggtc atgctgataa agacgggaat aatccctacc
    14041 gggttgacca gcgcaaataa cccgatgaaa aatttgaagt aaacgggaaa atcaaaaagg
    14101 gtctgaatca cggttagctc cgaagcaaaa gccggataat gttagccata aataaggttg
    14161 aaaagacgcg ctgacaatac gccttttgac agcatttttc acctcctaac tacttaaaat
    14221 tgctatcatt cgttattgtt atctagttgt gcaaaacatg ctaatgtagc caccaaatca
    14281 tactacaatt tattaactgt tagctataat ggcgaaaagc gatgctgaaa ggtgtcagct
    14341 ttgcaaaaat ttgatttgga tcacgtaatc agtacccaga agtgagtaat cttgcttacg
    14401 ccacctggaa gtgacgcatt agagataata actctaatgt ttaaactctt ttagtaaatc
    14461 acagtgagtg tgagcgcgag taagcttttg attttcatcg gttaagcaaa tcatcaccgc
    14521 actgactata ctctcgtatt cgagcagatg atttactaaa aaagtttaac attatcagga
    14581 gagcattatg gctgttacta atgtcgctga acttaacgca ctcgtagagc gtgtaaaaaa
    14641 agcccagcgt gaatatgcca gtttcactca agagcaagta gacaaaatct tccgcgccgc
    14701 cgctctggct gctgcagatg ctcgaatccc actcgcgaaa atggccgttg ccgaatccgg
    14761 catgggtatc gtcgaagata aagtgatcaa aaaccacttt gcttctgaat atatctacaa
    14821 cgcctataaa gatgaaaaaa cctgtggtgt tctgtctgaa gacgacactt ttggtaccat
    14881 cactatcgct gaaccaatcg gtattatttg cggtatcgtt ccgaccacta acccgacttc
    14941 aactgctatc ttcaaatcgc tgatcagtct gaagacccgt aacgccatta tcttctcccc
    15001 gcacccgcgt gcaaaagatg ccaccaacaa agcggctgat atcgttctgc aggctgctat
    15061 cgctgcaggt gctccgaaag atctgatcgg ctggatcgat caaccttctg ttgaactgtc
    15121 taacgcactg atgcaccacc cagacatcaa cctgatcctc gcgactggtg gtccgggcat
    15181 ggttaaagcc gcatacagct ccggtaaacc agctatcggc gtaggcgcgg gtaacactcc
    15241 ggttgttatc gatgaaactg ctgatatcaa acgtgcagtt gcatctgtac tgatgtccaa
    15301 aaccttcgac aacggcgtaa tctgtgcttc tgaacagtct gttgttgttg ttgactctgt
    15361 ttatgacgca gtacgtgaac gtttcgcaac ccacggcggc tatctgttgc agggtaaaga
    15421 gctgaaagct gttcaggacg ttatcctgaa aaacggtgcg ctgaacgcgg ctatcgtagg
    15481 tcagccagcc tataaaattg ctgaactggc aggcttctct gtaccagaaa acaccaagat
    15541 tctgatcggt gaagtgaccg ttgttgatga aagcgaaccg ttcgcacatg aaaaactgtc
    15601 cccgactctg gcaatgtacc gcgctaaaga tttcgaagac gcggtagaaa aagcagagaa
    15661 actggttgct atgggtggta tcggtcatac ctcttgcctg tacactgacc aggataacca
    15721 accggctcgc gtttcttact tcggtcagaa aatgaaaacg gctcgtatcc tgattaacac
    15781 cccggcttct cagggtggta tcggtgacct gtataacttc aaactcgcac cttccctgac
    15841 tctgggttgt ggttcctggg gtggtaactc catctctgaa aacgttggtc cgaaacacct
    15901 gatcaacaag aaaaccgttg ctaagcgagc tgaaaacatg ttgtggcaca aacttccgaa
    15961 atctatctac ttccgccgtg gctccctgcc aatcgcgctg gatgaagtga ttactgatgg
    16021 ccacaaacgt gcgctcatcg tgactgaccg cttcctgttt aacaatggtt atgctgatca
    16081 gatcacttcc gtactgaaag cagcaggcgt tgaaactgaa gtcttcttcg aagtagaagc
    16141 ggacccgacc ctgagcatcg ttcgtaaagg tgcagaactg gcaaactcct tcaaaccaga
    16201 cgtgattatc gcgctgggtg gtggttcccc gatggacgcc gcgaagatca tgtgggttat
    16261 gtacgaacat ccggaaactc acttcgaaga gctggcgctg cgctttatgg atatccgtaa
    16321 acgtatctac aagttcccga aaatgggcgt gaaagcgaaa atgattgctg tcaccaccac
    16381 ttctggtaca ggttctgaag tcactccgtt tgcggttgta actgacgacg ctactggtca
    16441 gaaatatccg ctggcagact atgcgctgac cccggatatg gcgattgtcg acgccaacct
    16501 ggttatggac atgccgaagt ccctgtgtgc tttcggtggt ctggacgcag taactcacgc
    16561 catggaagct tatgtttctg tactggcatc tgagttctct gatggtcagg ctctgcaggc
    16621 actgaaactg ctgaaagaat atctgccagc gtcctaccac gaagggtcta aaaatccggt
    16681 agcgcgtgaa cgtgttcaca gtgcagcgac tatcgcgggt atcgcgtttg cgaacgcctt
    16741 cctgggtgta tgtcactcaa tggcgcacaa actgggttcc cagttccata ttccgcacgg
    16801 tctggcaaac gccctgctga tttgtaacgt tattcgctac aatgcgaacg acaacccgac
    16861 caagcagact gcattcagcc agtatgaccg tccgcaggct cgccgtcgtt atgctgaaat
    16921 tgccgaccac ctgggtctga gcgcaccggg cgaccgtact gctgctaaga tcgagaaact
    16981 gctggcatgg ctggaaacgc tgaaggctga actgggtatt ccgaaatcta tccgtgaagc
    17041 tggcgttcag gaagcagact tcctggcgaa cgtggataaa ctgtctgaag atgcattcga
    17101 tgaccagtgc accggcgcta acccgcgtta cccgctgatc tccgagctga aacagattct
    17161 gctggatacc tactacggtc gtgattatgt agaaggtgaa actgcagcga aaaaagaagc
    17221 cgctccggct aaagctgaga aaaaagcgaa aaaatctgct taatcagtag cgctgtctgg
    17281 caacataaac ggccccttct ggggccgttt ttttgtttac ccaaagcaac ttttccataa
    17341 accgacagca ttagccttca tcatatttgc gacgatgtat aacgcctaca cacagcgata
    17401 ttgtacttta caggtcatta gtcaacgtcg gtgcttaagc gctctgtgag gcctatagcg
    17461 gcgttaaaaa ctaccgcgaa gggtgtataa cccagaagaa gtgcgtaaaa cgaactgaca
    17521 ggataaaaac gccctgctca ccctgtcagt aaagaaattc ttattaatcg tggcgatgcc
    17581 tttcctgaat agccgttaat gagccgactt gtaacgcctc tttatagtgt ttacggcata
    17641 cagaaacgta tcgttcatta ccaccaataa ccacctgctc accttcgtta taaggtctgc
    17701 ctgcttgatc aagacgcagc accatgcttg ctttacggcc acaaaaacag atggttttta
    17761 attcaaccag tttgtcggac catgccagta agtattggct gccaataaat aattcacctc
    17821 gaaaatcggt acgtaaacca taacaaagta cgggtatatc gagttgatcg acaacctccg
    17881 ataattcata tacttgttgt ctggttaaaa actggcattc atcaaccagt acgcaatgaa
    17941 ttgcctgctg ttcatgttcc gcacgaatct catcaaataa tgatgaattt tggttaaata
    18001 attttgcagg cgatgacaaa cctatacgcg aactgacttt cccggcacca aagcgatcat
    18061 caatttctgc cgtatatacg acagtgcgca tgccgcgttc ctggtaattg tatgaagatt
    18121 gcaacaatgc tgtagactta cccgcattca ttgcggaata gtagaaatat agctgtgcca
    18181 tcagccacag gccctcaatg atatgaaata aagttggctg gagtttatca taattcgtca
    18241 gtttttcagc tcaacagtat gcacagaagt ggtatatgcg ttctccctta cgaagccttg
    18301 cataatcctt ctgagctatc attacaactg cctcgcttgt tataagcggg taaatgactg
    18361 ctggtaacta ttcacaatct ttaacctgtt gcgcaagtaa tagccctctg ttgacctcca
    18421 ggagatagtg caatactaag tccctgctct tattgcgact gttctacttt tcatcattcg
    18481 cttaataggg aattctcgta aacacgacta atacagaaga ctgaaaggtc gtcagcctac
    18541 gataatctcc ccataaaatg tgacatgaat caggaagttt taacctcacg tgctgcgaaa
    18601 tcatcggtac aaatagggct atatgccgcg tcttttctgg ctaattttat gaaaagatat
    18661 ttattggcgg cacaaaataa agaacaattt tgaattcctt acattcctgg ctattgcaca
    18721 actgaattta aggctctatt attacctcaa caaaccaccc caatataagt ttgagattac
    18781 tacaatgagc gaagcactta aaattctgaa caacatccgt actcttcgtg cgcaggcaag
    18841 agaatgtaca cttgaaacgc tggaagaaat gctggaaaaa ttagaagttg ttgttaacga
    18901 acgtcgcgaa gaagaaagcg cggctgctgc tgaagttgaa gagcgcactc gtaaactgca
    18961 gcaatatcgc gaaatgctga tcgctgacgg tattgacccg aacgagctgc tgaatagcct
    19021 tgccgccgtt aaatctggca ccaaagctaa acgtgctcag cgtccggcaa aatatagcta
    19081 cgttgacgaa aacggcgaaa ctaaaacctg gactggccag ggccgtactc cagctgtaat
    19141 caaaaaagca atggatgagc aaggtaaatc cctcgacgat ttcctgatca agcaataatc
    19201 ttttgtagat tgcacttgct taaaatcccg ccatcggcgg gattttttat tgtccggttt
    19261 aagacaattt aataagaata agctataaaa aaacggcgtc gattgctcaa cgccgtttcg
    19321 tggataacac cgatacggat gttacttctt aatgcccatc tcttcttcaa gccaggcttt
    19381 aaattccgtg ccaagggtgt tatgacgaat accgtattca acgaaggcct gcatgtaacc
    19441 taatttatta ccgcagtcat ggctcttccc tttcatatga taggcttcaa ccgtttcttt
    19501 ttcgattagc atatcaattg cgtcggtgag ctgaatttca tcaccagctc ccggaggggt
    19561 ttttgccagc aacggccaaa tatccgcact aagtacgtaa cgacccacaa tagcgagatt
    19621 agacggcgca acatccgctt ttggtttttc aaccacgcca accatcggta cgctttcacc
    19681 cggcgctaat tcaacgcctt tgcaatccac aacgccatat gcggtcacat cagcaaccgg
    19741 ttcaaccatg atctggctat gacccgtttc atcgaagcgg cggatcatct ctgccaggtt
    19801 atcctgtgac aaatcggatt catattcatc cagaataacg tcaggcaaaa taacagccac
    19861 aggttcatca cccactaccg ggtgagcaca caataccgcg tgtcccaggc ctttcgccag
    19921 cccctgacga acttgcataa tagttacgtg cggcggacaa atagactgca cttcatcaag
    19981 cagttgacgt tttacacgtt tttccagcat tgcttccagt tcaaaactgg tatcaaagtg
    20041 gttttcaata gagtttttag atgagtgtgt aaccagcaca atttcagtaa tgccagccgc
    20101 aatacattca ttcacgacgt attgaattaa tggcttatcg acaagtggca gcatctcttt
    20161 cgggatggct ttcgtcgccg gcaacatcct ggttcctaat cccgcaacgg ggataacggc
    20221 ttttttgact ttcgtattaa tggcagccat ttaaattctc ctggactgtt cgtgttttga
    20281 acgtgttcat atttctgtat cgcatcccag tatatcagca ccccggaggg gctaagttct
    20341 gaaaaggatg cgagttagca taattaagac aattacttat acgaatggcg acgatagtac
    20401 caccagccga cattagctgg taatgcaaat ttagcccgcg ttatcgtttg ctcattctgc
    20461 agacaacatc aagcgcagtc gaccaccggt tccccatatt tggcattgcc aggcatcgca
    20521 tcgctggctc aattgattca aataagcgtt acctaaagtg cctaacggaa caccattact
    20581 gatttgcacc tggtgttcgc cggtatttaa cgtcgcattc agacccgcag aaaccagaat
    20641 gagatttttc agttcgcgat gataatagcc aactaatagc ggaaactgcc ccggcagatt
    20701 ggcctgacgg agtaaatggt ttacctgctt caataacgcg cccaactctg gcaaccgttg
    20761 attttggtgt gcaagctgtt cctgtaataa tccgttaaac aatgcgcgta ataacaaggc
    20821 agcaagtacg ccattatgtc cagctcgggt tacatcaagg caataaaatg ccagatcgtt
    20881 ttccgaaagt gcggcaatat caagcaccag gccgggtttg tccgcggcaa ccaattgacg
    20941 gtaattaacc cggcaatggg aaatcacctg ctgaaccggc ggttgtagtt cctgtaataa
    21001 tttcgccgct gcggcagggt tatcaaccat tgcatcccag tcgcgaaaaa gcctttcctc
    21061 ttcctcaacg cgcgaattaa acatgctggg atagagacag gcaaaaacca tctcgcgcaa
    21121 gcgattcaga tctttaactg gtttcagcaa aacatcttca acgcccagac gtaacgcttt
    21181 ggcaatatct gccatatttt cagtggcaga tatcaccaga actggggtct ggtcgcctct
    21241 gttacgtata tgctccagca gtttaagccc gttcattcgt ggcatcgcga tatcacatat
    21301 catcaggtct ggagtgaaac ctcccagcaa ctcaagggca tccaccccat cagccgccag
    21361 taccgttgtc gctcccaatg aggaaaacca cgaatccaga agcgagcgaa atacctgctc
    21421 atcttcaaca atgagaatct gttttccgac caatggctgc gtcatgttct ctcccctgac
    21481 tggctttact caatagtggc atgctattgc cacctgcgcc tgtcagaatt aacttaagtg
    21541 tagataaaaa ttctggtcaa atgttagtgc gtaccaacgg caaaagttcg tccattttct
    21601 tttccactgc cagctgtccg gccgcaatgg cagcgtgcgc gcgatggaaa tcaagcgtag
    21661 atatttgcgg gcaaacaggt tgaatcagaa tatcgggcgg atcacctgcc atgcggttcc
    21721 ttttaaggcg gttctccagc acctggatag aagtggtcat aatctctgtt gccgttggtg
    21781 ctgtcactgc ccgacgtgtc gttatgctgc ctaacctttc tttcagacgc gcatgccacg
    21841 gcagagaatc accattctcg ctattttctt cactgacatt aaaggagagc aaatcttgtt
    21901 gcatcaaatg agcatcgtgc tgcaggtcga ccgctatcac aatatcagca cccaatgcac
    21961 gcgtgagcga aataggaatt gggttaacga ctgccccatc aaccagccag tagccgttat
    22021 gcgcaacggg tgccattagt cctggaatac tgcatgatgc gcgaatagca agatggagat
    22081 cgccttcagt aaaccataat tcacgccccg tacttaaatt ggtggcaaca gccgcgaagc
    22141 gacgggaaca attttcgatc tctgtttccg gcattatttc gcggtattga ttgaagacac
    22201 gctcgccgcg cagtaaccca ccgcgctgcc aggagagatc catcaggcgt aaaacatccc
    22261 aataactgaa agaggttacc caatcttcca gcgcagataa tcgatcgcat gcataggccg
    22321 cgcccaccag cgaaccaatt gaacatcctg caacgatatc aatttcaata cccacttttt
    22381 ttagcgcatt aataacgcca atatgcgacc aacctctcgc cgcgccagat cccagcgcca
    22441 gccctatttt tatctttctc attattcccg caagattccc ctggaatgca atcgtagcca
    22501 catcgctact gctcggttaa catagtgcta ctcaggcggt taatacgccg ttattgtttc
    22561 cagggagatt atttgtgtct cagctttgtc cctgtggtag tgctgtcgag tatagcctat
    22621 gttgccaccc ttatgtgtct ggtgaaaagg ttgcacctga tccagaacac ctcatgcgtt
    22681 cgcgttactg cgcttttgtg atgcaagacg cagattattt aataaagacc tggcatccat
    22741 cttgtggagc agcggcgtta cgtgccgaat tgattgccgg atttgcgcat actgaatggt
    22801 tgggattgac cgtatttgaa cattgttggc aggacgccga caatatcgga tttgtcagtt
    22861 ttgttgctcg atttaccgaa ggtggcaaaa caggtgcaat aattgaacgt tcgcgatttt
    22921 tgaaagaaaa cggtcaatgg tactatattg acggtacacg tccgcagttt ggccgcaacg
    22981 atccctgccc ttgtggttca ggtaaaaaat ttaaaaagtg ctgcggccaa taatggttga
    23041 cggtacggtt tagcaaacac tctcaacaag gtttttccag caatgcattc actccaacgt
    23101 aaagttttgc gtactatttg tccggaccaa aaaggtctga tcgcacgtat taccaatatt
    23161 tgctacaagc acgagttaaa tatcgtacag aacaatgaat ttgttgatca ccgtaccggg
    23221 cgctttttta tgcgcacgga actggaaggg atttttaatg attccaccct gctggcggat
    23281 ctcgatagcg cattgccaga aggctccgtg cgtgagctga atcctgccgg tcgtcgccgg
    23341 atagtgattc tggtcactaa agaagcgcat tgccttggcg atttgttgat gaaagccaat
    23401 tatggcggcc tggatgtcga aatcgcggca gtgattggta accacgatac tttacgttct
    23461 ctggttgagc gttttgatat accgtttgag ctggtaagcc atgaagggtt aagccgcaac
    23521 gagcacgatc aaaagatggc ggatgccatt gatgcttatc aacctgacta cgtggtgctg
    23581 gcgaagtata tgcgggtatt aacaccggaa tttgtgtcac gcttcccgaa taagatcatc
    23641 aatattcacc attccttcct gccagcgttt atcggcgcac gtccttatca ccaggcctat
    23701 gaacgtggcg tgaagattat tggcgcaaca gctcactatg ttaatgacaa tctggacgaa
    23761 ggcccaatca tcatgcagga cgttattcat gtcgatcata cctacacagc tgaagatatg
    23821 atgcgcgcag gtcgtgacgt cgagaaaaac gtcttaagtc gcgcgctcta caaagtactg
    23881 gcgcagcgcg tctttgttta cggtaatcgg acgattattc tttaatcgcc agcaaaaata
    23941 actggttacc tttaatccgt tacggatgaa aattacgcaa ccagttcatt tttctcaacg
    24001 taacacttta cagcggcgcg tcatttgata tgatgcgccc cgcttcccga taagggatca
    24061 ggccagtaaa aagcattacc ccgtggtggg gttcccgagc ggccaaaggg agcagactgt
    24121 aaatctgccg tcatcgactt cgaaggttcg aatccttccc ccaccaccat cacttttcaa
    24181 aagtccctga actctcaaac gaatccgcaa ttaaatattc tgcccatgcg gggaaggatg
    24241 agaagcttcg accaaggttc gactcgagcg ccagcgagag agcgttgccg caggcaacga
    24301 cccgaagggc gaagcgcgca gcgctgagta atccttcccc caccaccatc gcttcctgaa
    24361 ataatcaaaa catgttactc cgagctaatt ttgcccgatg gcaccacgtt taccggacct
    24421 acaatatcct cccctgtagg tcggataagc gaagcgcatc cgacaactca ccccgcaccg
    24481 tgcccataaa aaaaccccgc cgaagcaggg tctatgtgaa ctaaaattcg cttagtgacg
    24541 agcgcgcacc agctggtact tacgtgtcag atactccacc ggtacgctcc agatgtgcac
    24601 cagacgcgag aacgggaaca gcaggaataa cgtcatcccc aacaccaggt gcagacggaa
    24661 gataaacgcc acaccatcaa ggtgttgaga agcgccaccg tggaaggtca ctaccgactg
    24721 cgcccagcca accagtttca tcatctcgct accgtccata tgttgggcgg agaacggaat
    24781 ggtcaacagg cccagcgcgc actggataac gagcagcgac aggatcagta tatccgctcc
    24841 ggtagtcgtt gcacgcacgc gtgggctgaa cagacgacgt ttaagcaaaa gcacgccgcc
    24901 aatcagacac agcacgccgc tggcaccacc agcaaacatc gccattttct gtttcacttc
    24961 aatcggcagc caggcttcat acatccagtg cggcgtcagc ataccgaaga agtgaccgac
    25021 aaaaatcccc agaataccga tatggaacag gttcgacgcc aggttcatcc ctttgcgatc
    25081 cagcatctgg ctggacgccg cgcgccaggt gtactgcccg tagtcataac gcagccagct
    25141 accaatcagg aagaccgccc cggctatata cgggtagata tcaaagaaga acatattcag
    25201 gaattgcatt agtgctgtcc tccggtggtg atattcagat attgcggcgc gacggcaccg
    25261 gcaaagcgac gctgatgagc agtgattgct gaatcaccgc agcctttgtc agcaaagaat
    25321 ttaacctgct cttcttccca aacagcatcc agcgcctgcg gcgtatcatc gcgcgcttcg
    25381 tcggcaattt tttccgccac tttgtcgctg tcgatagcgg tattggccaa tttcagcagc
    25441 agatcaaaca gcacggcata acggctttca cgctgttgca gacgcgcgct cagcaatgcc
    25501 agaatcggcg cgatatcttt caaaccttcc acggcttcgc tttgcggcag ctgcgacagg
    25561 tactccagat acagcggcag atgatccggc aattcgcggc tgtttaactg caagccgtgc
    25621 tgctcgtact gcgccagcag gtccaccatc gcctggccgc ggtcgcggga ttcgccgtgc
    25681 acatgttcaa acagcaacag tgacgtggcg cggccacggt cgaacagttc gctgtactgc
    25741 gcctgggcat cgagcggatc catcgtcgtt aaatcgcgca ggaaaatgcc cagcgcatgg
    25801 gcatcctctt ttggcagatt tttcgacgcg gcaatcgcct caaacatttc ttgttgatgc
    25861 tgccataagg cagcatccgg atattcaagg agacgcgata caatcacgag ttcgatcatg
    25921 gatgcggctc cgttttgctg gtcacatcga tggcatcgat acgacggctg ttgaacagat
    25981 tgaatttggt atctgaaccg tggcagccat caccaaaggt aaagccgcag ccatttttct
    26041 ccgggaaggc ttcccgtgcc agttcacgat gactactcgg caccacaaag cgatcttcgt
    26101 agttagcaat cgccagataa cggtacatct cctgtgcctg ggcttcggtc agaccgacct
    26161 cttccagcgc acgggtatcc actttcccgt caacggtttc agcacgtttg taatgacgca
    26221 tcgccagcat acgtttcagc gcgcgcagta ccggtttggt atcaccggcg gtcagcagat
    26281 tcgccagata ctgtaccgga atacgcaggc tttcgacgtc tggcagaatg ccgttgctac
    26341 ccagctcacc cgcgtctgct gcagactgaa tcggagacag aggcggcacg taccagacca
    26401 tcggcagtgt gcgatattct ggatgtagcg gcagcgccag tttccattcc attgccattt
    26461 tataaaccgg cgactgctgt gcagcttcaa taacgctcag cggaataccg tctttaatcg
    26521 cctgctcgat gactttcgga tcgttcggat cgaggaacac ctccagctga cgctggtaga
    26581 gatctttctc gttctcggtg ctggctgcac gttcaatcgc gtcggcatcg tacagcagca
    26641 cgccaagata acggatacga ccgacacagg tttctgagca cacggtcggc tgacccgctt
    26701 caatacgcgg atagcagaag atgcacttct cagacttacc gctcttccag ttgaagtaga
    26761 tttttttgta cgggcatccg gtgatgcaca tacgccagcc acggcattta tcctggtcga
    26821 tcaggacgat gccatcttct tcacgcttgt aaatcgcacc gctcgggcag gtcgccacac
    26881 atgccgggtt caggcagtgt tcgcacaggc gcggcaaata catcatgaag gtgttttcga
    26941 actggctata catcgccttc tggatgttgt cgaagttctt gtctttcgcc agcttgtcaa
    27001 actcaccgcc caggtcatct tcccagttcg gccctttttc gattttcgcc atccgttccc
    27061 cggtaatcag cgaacgcgga cgggcaatcg gctgtgattt gctgccttcc ggcgcggtat
    27121 gcaggttctg ataatcaaaa tcgaacggct cgtaataatc gtcgatcccc ggcaaatgcg
    27181 ggttagcaaa gattttaccc agcagcatgg ctcggttacc catgcgcggc tgcagtttgc
    27241 cattgatttt acggatccag ccgcctttgt atttttcctg gttttcccag tcagtcggga
    27301 agccctggcc cggcttggtt tccacgttgt tgaaccacgc gtattccacg ccttcacggc
    27361 tggtccagac gtttttacag gtaactgaac aggtgtggca gccgatgcac ttatcgagat
    27421 tcagcaccat gccgacttgt gaacgaattt tcattttacg ctctcctgta cctggtcatt
    27481 gccttcgcca tctaaccagt caatgttctt catcttacgc actacaacaa actcatcgcg
    27541 gttagaacct acggtgccgt agtagttaaa gccgtatgcc agatgggcat agccgccgat
    27601 catatgcgtc ggtttcggcg tgatacgggt gaccgagtta tggataccac cacgctgttg
    27661 ggtaatttcc gaaccaggca ggttaacgat acgttcctgc gcgtggtaca tcatggtcat
    27721 ccctgccgga acacgctggc tgacaaccgc acgggcagtc agagcgccgt tgctgttgaa
    27781 gacttcaatc cagtcgttat cggcgatacc cagctctttg gcatcggctt cactcaacca
    27841 gaccaccgga ccaccgcgac ctaaagtcag catcagcagg ttgtcgctgt aggtggagtg
    27901 gataccccac ttctggtgcg gcgtcaggaa gttgagcgct ttttccgggt tgccgttgga
    27961 tttctggcct atcacttctt tcaccgaacg ggtgtcgatc ggcggacgat aaaccagcag
    28021 gctttcaccg aaatcacgca tccactggtg atcctgatac agttgctgac gaccagagag
    28081 cgtacgccat gggatcagct cgtgaacgtt ggtgtaaccg gcgttgtaag aaacgtgttc
    28141 atcttccaga ccagaccagg tcgggctgga gataattttg cgcggctgtg cctgaatatc
    28201 gcggaagcgg atcttctcgt cttctttatt cagcgccaga tgcgtatggt cacgaccggt
    28261 aaactcgctc agggcagccc aggctttcac ggctacctga ccgttggttt ccggtgccaa
    28321 cgtcaggatc atctctgccg catcaattgc ggtgttcagc atcggctggc ctttcgccgg
    28381 accttccgct ttggtgtagt tgagcttacg cagcagatcc atctcgctct gggtgttcca
    28441 ggcaatccct ttaccgccgt taccgatttt ctccatcagc gggccgatag aggtaaagcg
    28501 ttcgtaagtt gccggataat cgcgctctac gaccataatg tgcggtgcag ttttgcctgg
    28561 gatcaggtcg cattcgcctt ttttccagtc tttcacatcc agcggctgcg ccagttcagc
    28621 ggcagagtcg tgctggatag gcagcgtgac gatgtcggtt tctttaccca gatggccgac
    28681 gcacacttcg gagaatttct tcgcgatggc tttgtagatt tcccagtcgc ttttcgcttc
    28741 ccaggccgga tcgaccgccg cagacagcgg gtgaataaac ggatgcatat ccgatgtatt
    28801 catgtcgtct ttttcgtacc aggtcgccgt cggcaaaatg atgtcggaat agagacaggt
    28861 gctcgacaga cggaagtcca gcgtaaccac cagatccagc ttgccttcca gaccattgtc
    28921 ctgccagtcc acttcttccg gcttcacgcc gccctgctgc cccagatctt taccctggat
    28981 accgtgctcc gtccccagca ggtacttgag cataaactca tgacctttac cggaagaacc
    29041 gagcaggtta gaacgccaga tgaacaggtt gcgcgggtgg tttttaccgt tttctggttg
    29101 ttctgccgca aaacggatgg aaccctcttt cagggatttc accgtatagt ccaccggatt
    29161 catcccggct ttttccgctt cgccagcaat agtcagcggg ttagtgccta actgcggtgc
    29221 agacggcagc cagcccatgc gttctgcacg gacgttaaag tcgatcaagt gtccggtata
    29281 gcgagattta tccgccatcg gcgacagcaa ctcgtctgcg gtcacggttt cataacgcca
    29341 ctggctggag tggttataaa aataagaggt gctgttcatg tgacgcgcag gacgctgcca
    29401 gtcaagggca aacgccagcg gctgccaacc ggtttgcgga cgcagttttt cctgacctac
    29461 atagtgcgcc cagccgcccc cactctgacc gacacagccg cagaaaatca gcatgttgat
    29521 cagaccacga tagttcatat cgaggtgata ccagtggttc agccccgcac cgacgataat
    29581 catcgaacga ccgtgcgttt tatcagcgtt atcggcaaat tcacgggcga tgcgaataat
    29641 ctggctgcga gaaacgccgg taatctgctc ggcccaggcc ggggtataag ctttcacatc
    29701 gtcatagctg gttgcacagt taacgtcgtt caggccacgt tccagaccgt agtttgccag
    29761 cgtcagatca taaacggtgg tcaccagggc ggtgctgcca tcagccagtt gcaggcgttt
    29821 caccggcagt ttgtgcagca gcacgttttc cagttccact ttgttgaagt gttccgtgcc
    29881 gtcgccacca aagtacggga agcctacctc agcgatctca tcctggctac ccagcaggct
    29941 cagttgcagc tcggtttctt cgccagtttt gccgtcgcgc tgctcaagat tccatttgcc
    30001 cttctcgccc cagcggaagc caatagaacc gttcggcgca accatttcgc cattggtatt
    30061 aaaggcgaca gttttccatt ccggattgtt ttcctggccc agcgcatcaa ccagatcagc
    30121 agcgcgcagc atacgacctg cagcgtagta accgtcgcgt tcttccagca tcaccagcat
    30181 cggcatgtcg gtgtagcgac gcacatagtc ggtgaaatac tggcttgggt tgtcgagatg
    30241 gaattcacgc agcattacgt ggcccatcgc cagcgccatt gccgcatcgg tgccctgttt
    30301 cggtgccagc cacagatcgc acagtttggc gatttcagcg tagtctggtg tgacggcaac
    30361 agttttggtg cctttgtaac gcacttcagt aaagaagtga gcatctgggg tacgcgtctg
    30421 cggcacgttt gaaccccagg cgatgatgta gctggagttg taccagtcag cagattccgg
    30481 tacgtcagtc tgctcgcccc aggtttgcgg cgacgcagga ggcaggtcgc agtaccagtc
    30541 gtagaagctt aagcaagtac cgccaatcag cgagagatag cgtgcacccg atgcgtaaga
    30601 aaccatcgac attgccggaa ttggcgagaa accagcaaca cggtccgggc cgtagttttt
    30661 gatggtgtaa acgttagatg cggcgatcag ttcgttcacc tcctgccagg aagaacgaac
    30721 aaaaccaccg cgtccacgcg cctgcttaaa gcttttcgct ttatcggcgt cttcaatgat
    30781 agaagcccat gcctcaaccg gatcgctatg cagcgccttc gcttcacgcc acattttcat
    30841 caggcgtttg cgcatcatcg ggtatttcag gcggttggca ctgtaaagat accaggagta
    30901 gctggcaccg cgcgggcagc cgcgaggttc atggtttggc agatccggac gggtacgcgg
    30961 atagtcagtc tgctgggttt cccaggtgac cagaccgttt ttgacgtaga ttttccagct
    31021 gcaggagccg gtgcagttta ccccgtgggt agagcggacg attttgtcat gctgccaacg
    31081 ctggcgatat ccatcctccc agtcacggtt ggtattgaga agctggccat gcccatcggc
    31141 aaaggtttca cccttctgtt tgaagtagcg aaaccggtcc aggaatttac tcatcggttt
    31201 tctcctgtgg gagcctgtcg gccctctgat aaatcgacat tgctaatgtt taagggcaag
    31261 gtaacgctct gaaacagcgt gggaattgat aacgatcaag agtgatgggg atgaaaaata
    31321 aagtaaattc ctaagatacc cctttaggat aattaacagt tacagcataa catgctgtaa
    31381 attaagactt tttatacctg tagttattta aactattaag atttttatgc aaatgggtat
    31441 taaggagtat tccccatggg taacatatta atggctctta atgaaatccg gcgaaataaa
    31501 ccatataaca ttagtatgtt gtaactaaat gacgcataaa aaaagcgcga catcatgccg
    31561 cgcaaaggat aatcaaacat taagatttat tttttagaat gccgaccata taccgcccag
    31621 gtaatcacta cgcaggcgat atagaaaatc aaaaatacct tcattgcgcc gactggcgaa
    31681 cccgttaatg ccagcgagct accaaacgct ttcgggataa agaagccacc aatcgcgcca
    31741 atcgcagaga tgaaacccag cgccgccgcc gtgtcggttg ccgcttcacg catcgcacgt
    31801 tcgtcagaac ccccttctgc tttcacgcga tccattgtca gtttacggaa gatcactgaa
    31861 atcatctgga aagtggaacc actacccagc ccagctgtca ggaacagcgc caggaagacc
    31921 gcgaagaacg ccatgaagct tccgccctgc ccgtcagtcg gtaaggtcag gaacagcaga
    31981 ccgctgaaaa tcgccatcag aataaagttc accagcgtga cacgagttcc gcccagacgg
    32041 tcagataatg caccacctgc agaacgcgcc agcgcaccaa taaacggccc gaagaaagcg
    32101 tattgcagaa tctgaacatc cgggaactgc gtttttgaca gcatcgcaaa gcccgcggag
    32161 aagccgataa aggagccgaa ggttgccaga tacagcaggc tcataatcca cagatgaccc
    32221 cgtttgagta ccggcaactg ctccttgatg gaggctttcg aggtagcaag atcgttcatg
    32281 ccaaaccacg ccgcaatggt gaagatggca aggaacggca cccatatcca ggacgcattc
    32341 gccagataca gctcagtccc atccggctgt ttgacgccct ggctaccaaa tactgcgaaa
    32401 atcgacagtg ataccaccag cggagcaacc aactgcatga cgctgacgcc catgttgccc
    32461 agaccaccat tcagacccag cgcgccaccc tgcttctgtt tcggaaagaa gaagctgatg
    32521 tttgccatac tggaagcgaa gttcgcgcca gcaaagccgc acagcagaga gatgatgatg
    32581 aagacgctat aaggcgtgga ggtatcctgc acggcaaaac ccagccagac acaaggaata
    32641 atcagaatac cggtgctgaa cgccgtccag cgacgaccac cgaagatagg aaccataaag
    32701 gagtatggaa cacgtaataa cgcgccagaa accgaaggca gcgcagtcaa cataaatagc
    32761 tgatcggtcg taaaattaaa accgactttc ggtaagttca ccgcaacagc gctgaacaac
    32821 atccatacgc aaaacgccag cagcagacag ggaacggaaa tccacaggtt gcggctggca
    32881 atacgttgac cgcgttgttg ccagaacgca ggatcttccg gtcgccaatc tgtaatgaca
    32941 gctccagtag ccctttcggg ggcggatgag tgactcatag acacctctga tactcgtttc
    33001 gatgtctgcc accttagtgt ctgtagctaa aggcaatttg atgtaaatca aacgataaga
    33061 taactttatc tatcattgat atttatcatt acccatagtg agtacagtga tttcataaaa
    33121 attatgagat ttttcacggt gctgtagaat ccctaccctt accgatgtaa agcgactaac
    33181 cacacggcaa ataaggagta actctttccg ggtatgggta tacttcagcc aatagccgag
    33241 aatactgcca ttccagaatg tatcgtcaca ttcattaagg ttattgctca tttaaagcct
    33301 gaaggaagag gtttacatgc ttaaacgttg tctctctccg ctcaccctgg ttaatcaggt
    33361 tgcgcttatt gtgttgcttt ctactgctat tggactggca gggatggcgg tttctggctg
    33421 gctggtgcaa ggcgttcagg gcagcgccca tgcgatcaac aaagcgggat cgctgcgcat
    33481 gcaaagttac cgtctgttgg cagcagtgcc attaagcgag aaagacaagc ccttaattaa
    33541 agagatggaa caaacggcat ttagcgccga gttgactcga gcagcagaac gagacggaca
    33601 actggcgcaa ttacagggtt tacaagatta ctggcgtaat gaactgatcc ctgcgctgat
    33661 gcgggcgcaa aaccgagaaa cggtatcagc ggatgtcagc cagtttgttg ccgggcttga
    33721 tcagctggta tctggttttg accgcaccac ggaaatgcgt attgagacag tggtactggt
    33781 ccatcgggta atggcggtat ttatggcact tttactggtg ttcactatta tctggttgcg
    33841 ggcgcgactg ctacaaccgt ggcggcaact gctggcaatg gcgagtgccg tcagccatcg
    33901 cgattttacc caacgcgcaa acatcagcgg gcgcaacgaa atggcgatgc ttggaactgc
    33961 gttgaacaat atgtctgcag aactggccga aagttatgcc gtacttgagc agcgggttca
    34021 ggagaaaacc gccgggctgg agcataaaaa tcagatcctc tcttttttat ggcaggctaa
    34081 ccgccgtttg cattcccgcg ccccgctgtg tgaacgcctg tcacctgtac tcaacggctt
    34141 acagaattta accctgctac gtgatatcga actgcgggtg tatgacactg atgatgaaga
    34201 gaatcatcag gagtttacct gccagccaga tatgacttgt gatgataaag gctgccagct
    34261 ctgcccgcgc ggcgtattac ccgttggtga tcgcggcacg accctgaagt ggcggctggc
    34321 tgactctcat actcagtacg gtattttgct ggcgaccctg ccgcaggggc gtcatcttag
    34381 ccatgatcaa caacaactgg tggataccct ggttgaacaa ctcaccgcca cgctggcgct
    34441 ggatcggcat caggaacgtc agcaacagtt gatcgtgatg gaagagcgtg ccaccattgc
    34501 gcgcgaactg catgattcta ttgcccaatc tctctcttgc atgaagatgc aggtgagttg
    34561 tttacagatg cagggcgatg cgctgccaga aagcagccgc gaactgttaa gtcagatccg
    34621 taacgaactg aatgcatcct gggcgcagtt gcgtgaattg ctcaccacat tccgcttgca
    34681 gctcaccgag cctggattac gtccggcgct ggaggcgagt tgcgaagagt acagcgccaa
    34741 atttggcttc ccggtgaagc tggattatca attgccgcct cgtctggtgc cttcgcatca
    34801 ggcaatccac ttgttgcaaa ttgcccgtga ggcattaagt aacgccctca aacattcgca
    34861 agcgagtgag gtcgtggtga cggtggcgca aaacgataat caggtcaaac tgaccgtcca
    34921 ggataacggc tgcggcgtgc ctgaaaatgc catccgcagc aatcactacg gcatgataat
    34981 aatgcgcgac cgtgcgcaaa gtttacgagg cgattgccgc gtccgccgtc gtgaatcagg
    35041 tggcaccgaa gtggtggtca cctttattcc cgaaaaaact ttcacagacg tccaaggaga
    35101 tacccatgag taatcaggaa ccggctacta tcctgctgat tgacgatcac ccgatgctgc
    35161 gaactggcgt aaaacagctt atcagtatgg caccagatat caccgtggtt ggcgaagcga
    35221 gtaatggcga acagggtatt gaactggcgg aatctcttga tcccgatctg atcctgttag
    35281 atctcaatat gcccggcatg aacggtctgg aaacgctgga taaactgcgc gaaaagtccc
    35341 tctcagggcg cattgtggta ttcagtgtct ctaaccatga agaagatgtg gtcaccgcac
    35401 tgaaacgcgg cgcggatggc tatctgttaa aagatatgga accggaagat ctgctgaaag
    35461 cattgcatca ggcagctgct ggcgaaatgg tattaagcga agcattaacg cctgttctgg
    35521 ccgccagctt gcgcgctaac cgtgccacga ctgagcgcga tgttaaccag ttaaccccac
    35581 gcgagcgcga tattctcaag ctgattgccc agggtttgcc gaacaagatg attgcccgcc
    35641 gcctggacat caccgaaagc acagtaaaag tgcacgtcaa gcacatgctg aagaaaatga
    35701 agctcaagtc tcgcgtggaa gcagcggtat gggtgcatca ggagcgcatt ttctgattac
    35761 ggttcccagc gcagttcgtc gtttgacaat gcgtcgaacg gttcgacaag cgttagcgtg
    35821 atctcattgg aggagacacg ctgcccctgg ttatcttcca ccaccaccga caatcgccag
    35881 tgattgctcg ccccttcccc gttctgccag tcaggcatga tcagcgtcca gccctccgcg
    35941 ctgttggctt gtgcgcccgg cgtcaaactt aatatctgcg tatcgccctg ccaaatcagt
    36001 tgccgaatac cgtaacgact gcggatttgt aatttcagcg gcactgtttc gccaggtttt
    36061 agatcccacg gcggtgtcgc cagaaacacc gttaacgttt ttcgctgtcg gtactcaaga
    36121 gtcggtagat tatttcgctg cggattatca tagcgactac cacgtaacga ctgactttcg
    36181 gcaacctcgc ccgcagaaag ttgttttttg agcggtacac caaagcggta attaagattc
    36241 agcccgaggt tattttgatt ctcgccactt tcaccctgtt tatgctgggc cgtcacagtg
    36301 actaatggca caggggtgta atttaatccc agactcaacg cgacgggatt gtgataaccc
    36361 gtaccagagt taaacaaatc aacacgatca ccaaaatact gttctacgct gacactggta
    36421 ttgaggtgtt gatagaacgg catgcgcatc cgggctgtca ggtcgtaccc gcgcgccatc
    36481 cgttgttcct gcgtggctgt ctgttcatgc catgcagcaa acggctgata aaagtttgcc
    36541 gatagtcgca aatattcgcc ccacgcttcg gcaccaaagc ccgcacgctg aagattttcg
    36601 tccagcaagt tgtcataaaa agtgttataa cccaccagcc agctgccgcg cgcccagcgt
    36661 tgcccaacgc ccacattgct caccagccca tcatcctgct gagtaagacc aagctggctc
    36721 caggtgagat aacgatcatt atcttgtaac ggcacaaacc agcttccacg actgccggta
    36781 aaatgacctt cgttatccac tttgacgtca acactggcat ttccccacgg tgatagccac
    36841 gactctacgt gctgattaac ctgttgacta agcgcgtcgc ggacttttcc caatgcgaaa
    36901 gcttttgcct gctcgccagt atccagcccg ttatcattca tactggtttc gccgaaatct
    36961 ttcacaattt cagcaaagtg tttttcccca tcatgatttt caggcgccat gcctaaatcc
    37021 ggcagaccat cattgttatt atcaaaggga tttgccgctt tttgctcgaa ggtagattgt
    37081 gcgttagctg taccgcctgc cgccagcaag agtaaataaa acggaataat gcgaggaacg
    37141 aaacggctca aaacgtcggt aacttccggt aaaaaatgaa aaaccatagc cttaaagaat
    37201 aacgattact ggcaactttt gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg
    37261 tgaccgattg gtcagacgct gcagaagcag taagatagaa gatgagtgtc taattccctg
    37321 aatacaggag taaataatgc aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag
    37381 cgaatccttg tttaacagct tgcggctggc cattgcgtta cgagagcagg agagcaatct
    37441 ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc
    37501 aggggaaggc tacaacattc agcaaatgct ggagatcctt accgctcaga atgtaccggt
    37561 gaaattgtgc aaaacctgta ccgacgggcg cgggattagt acacttcctc tgattgatgg
    37621 ggtggaaatc ggtactcttg tggaactggc gcaatggacg ctgtcagccg ataaagtgct
    37681 cacattttaa tgtttgaggg ggcgtctgac gccccacgat gagggaagtt tactcaggcg
    37741 aatcccggcc gcaacacacc atccggataa ttctccgcca gcagtttttt taccgatacc
    37801 agcaaatcat tcagcccatc atcctgcatt cccagtttga taagctcctg ttccagtgaa
    37861 aacaggtatt gtgcgttggt tcccagcgga ccgctcgccg ccgcaatcaa cggcgcgatg
    37921 acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg ggtccataat aaacacaatg
    37981 gcgtttactg tgcgtccatc atcaagatca agctgacacc aggttggcag ataacagccg
    38041 gtaatcatct cgcgcttcca caacagggtt agttcctgct ccagcgtctc ttctggcagt
    38101 cgataggcga cgcctgtggt acgtccgccc tcttttagtg caagcatccg tcccggctgg
    38161 tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg cgcgatgcca tccaaccagc
    38221 gtaccggtgc acgactcggt aaactccagt gccggattcc acatcaacga tccataaccg
    38281 aagatccaca ccggtctctc atcaggtcga caagccagcg tcgccgccag cgaagccgcc
    38341 cgttgttctg ctgaccataa gagtgattct tcaatggcac caaatgccgt tttacaatcg
    38401 gcattcatca agaaatcacg cgttatcacc ttacacctcc gccactgcat acttccctgc
    38461 gacaactatt tggtgtcttc cggacatttt tactgctcat tttgaaacca ctaacgggac
    38521 aatatgcaat ccgcggacca cttgcaaggc aacaccgcac gaatagcttt aactaagcta
    38581 ccggttttac gattttttat gccatttatc atcatcccct ttggcatatt catgcttcac
    38641 agcagcccag gccactttat gcgcggtttc ttcgcgactg gcgtcatcac gccgatcttc
    38701 tttatcttta tattgatccc atgcgctgtt gaacgcttct ttatagatat cctgggcatg
    38761 agacggtaga acgtgcttta cgctttccgg caggtcgctt ttcgttttat acggcatcgt
    38821 tgactccttt tacaaagttg aacattaagt gtggtcaaca atatctactc ttgccaaaac
    38881 ataagaattt tcgtgtcaga ctgttcttaa gaaacgctaa aacaaacatt gtctaaacgt
    38941 attacttatt tttatgcccc aatgagtaaa aaatcgacag cggggtaaaa tttcgtaaaa
    39001 tgttaccgat atccagacac tttctgttat ttttcctacc tattttttac attttcgaca
    39061 aattcatctg caaagggata accataatgt caaatgctca agaggcggta aaaacccgcc
    39121 acaaggagac ttcgcttatt ttcccggttc tggcgctggt agtgctgttc ctgtggggaa
    39181 gcagccagac actaccagtg gtcattgcca tcaatcttct tgcgcttatt ggtattttaa
    39241 gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc ccatcgcctg ggagaacctt
    39301 acggttcgct tattcttagc ctttcagtgg ttattcttga agtcagtttg atttcagctt
    39361 taatggcaac ctgcgacgcc gcgccaacgc taatgcgtga tacgctctat tcaatcatta
    39421 tgattgttac cggtgggctg gttggctttt cattattgtt gggcggtcgt aagtttgcca
    39481 cccaatatat gaatctgttt ggtatcaagc agtatttaat tgcgctgttc cccctggcga
    39541 taatcgtact ggtatttcca atggctctgc ctgcggcgaa tttttcaacc ggtcaggcgt
    39601 tactggtagc attaatttct gcggcaatgt atggcgtatt tttgctgatc cagaccaaaa
    39661 cgcatcaaag tttgtttgtc tacgagcacg aagatgacag tgatgacgac gacccgcacc
    39721 acggtaaacc gtctgcccat agcagcctgt ggcatgctat ctggttgatt atccacctga
    39781 ttgccgttat tgcagtcacc aaaatgaacg ccagcccgct ggagactttg ctcgacagca
    39841 tgaatgcccc tgtcgccttt actggcttcc tggtggcact gttgattctg tcgccggaag
    39901 gtttaggtgc attaaaagca gtgttgaaca accaggtcca gcgcgcgatg aatctgttct
    39961 ttggttcagt gttagcaaca atttcgctta ccgtacctgt cgtcacgcta attgcattta
    40021 tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga aatggtggtg atggtggcct
    40081 ctttagtgct gtgccatatc tccttctcca ccggacgtac taacgtgctc aatggcgcag
    40141 cgcatctggc actgtttgcc gcctatttga tgacgatatt tgcctgacag ttatatctcc
    40201 tccgatgacg gaaatcgggg gagataatat aaattcctgc ttattgtatt tcttcatact
    40261 atctggacag gtcgttaatc agaattgcga gcctgtacca caacctgcac gaatccactg
    40321 caacagctgt tgaaaagccg gaatgtactc ttcattgcaa acatttcagg gatgaagtga
    40381 agatgaattg ag
//
LOCUS       DAZFFG010000027        40258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFFG010000027 DAZFFG010000000
VERSION     DAZFFG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.027"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(30..1889)
                     /gene="gss"
                     /locus_tag="ACHJ7J_001817"
     CDS             complement(30..1889)
                     /gene="gss"
                     /locus_tag="ACHJ7J_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO5000060.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2094..2960
                     /gene="yghU"
                     /locus_tag="ACHJ7J_001818"
     CDS             2094..2960
                     /gene="yghU"
                     /locus_tag="ACHJ7J_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO5000061.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIKYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3083..3331)
                     /gene="hybG"
                     /locus_tag="ACHJ7J_001819"
     CDS             complement(3083..3331)
                     /gene="hybG"
                     /locus_tag="ACHJ7J_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO5000062.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3465..3806)
                     /gene="hypA"
                     /locus_tag="ACHJ7J_001820"
     CDS             complement(3465..3806)
                     /gene="hypA"
                     /locus_tag="ACHJ7J_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5000063.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIIYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3799..4287)
                     /gene="hybE"
                     /locus_tag="ACHJ7J_001821"
     CDS             complement(3799..4287)
                     /gene="hybE"
                     /locus_tag="ACHJ7J_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO5000064.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4280..4774)
                     /gene="hybD"
                     /locus_tag="ACHJ7J_001822"
     CDS             complement(4280..4774)
                     /gene="hybD"
                     /locus_tag="ACHJ7J_001822"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO5000065.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4774..6477)
                     /gene="hybC"
                     /locus_tag="ACHJ7J_001823"
     CDS             complement(4774..6477)
                     /gene="hybC"
                     /locus_tag="ACHJ7J_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO5000066.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6474..7652)
                     /gene="hybB"
                     /locus_tag="ACHJ7J_001824"
     CDS             complement(6474..7652)
                     /gene="hybB"
                     /locus_tag="ACHJ7J_001824"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO5000067.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7642..8628)
                     /gene="hybA"
                     /locus_tag="ACHJ7J_001825"
     CDS             complement(7642..8628)
                     /gene="hybA"
                     /locus_tag="ACHJ7J_001825"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO5000068.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8631..9749)
                     /gene="hybO"
                     /locus_tag="ACHJ7J_001826"
     CDS             complement(8631..9749)
                     /gene="hybO"
                     /locus_tag="ACHJ7J_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO5000069.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9938..10225)
                     /gene="yghW"
                     /locus_tag="ACHJ7J_001827"
     CDS             complement(9938..10225)
                     /gene="yghW"
                     /locus_tag="ACHJ7J_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO5000070.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10344..11231)
                     /gene="yghX"
                     /locus_tag="ACHJ7J_001828"
     CDS             complement(10344..11231)
                     /gene="yghX"
                     /locus_tag="ACHJ7J_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO5000071.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11388..12428
                     /gene="gpr"
                     /locus_tag="ACHJ7J_001829"
     CDS             11388..12428
                     /gene="gpr"
                     /locus_tag="ACHJ7J_001829"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO5000072.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12468..12962)
                     /gene="yqhA"
                     /locus_tag="ACHJ7J_001830"
     CDS             complement(12468..12962)
                     /gene="yqhA"
                     /locus_tag="ACHJ7J_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO5000073.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13153..14037
                     /gene="yghA"
                     /locus_tag="ACHJ7J_001831"
     CDS             13153..14037
                     /gene="yghA"
                     /locus_tag="ACHJ7J_001831"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5000074.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQGVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14309..14734)
                     /gene="exbD"
                     /locus_tag="ACHJ7J_001832"
     CDS             complement(14309..14734)
                     /gene="exbD"
                     /locus_tag="ACHJ7J_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO5000075.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14741..15475)
                     /gene="exbB"
                     /locus_tag="ACHJ7J_001833"
     CDS             complement(14741..15475)
                     /gene="exbB"
                     /locus_tag="ACHJ7J_001833"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5000076.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15727..16914
                     /gene="metC"
                     /locus_tag="ACHJ7J_001834"
     CDS             15727..16914
                     /gene="metC"
                     /locus_tag="ACHJ7J_001834"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO5000077.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17054..17713
                     /gene="yghB"
                     /locus_tag="ACHJ7J_001835"
     CDS             17054..17713
                     /gene="yghB"
                     /locus_tag="ACHJ7J_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO5000078.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17753..18652)
                     /gene="yqhC"
                     /locus_tag="ACHJ7J_001836"
     CDS             complement(17753..18652)
                     /gene="yqhC"
                     /locus_tag="ACHJ7J_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO5000079.1"
                     /translation="MKREEICRLLANKVNNLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18846..20009
                     /gene="yqhD"
                     /locus_tag="ACHJ7J_001837"
     CDS             18846..20009
                     /gene="yqhD"
                     /locus_tag="ACHJ7J_001837"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO5000080.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20114..20941
                     /gene="dkgA"
                     /locus_tag="ACHJ7J_001838"
     CDS             20114..20941
                     /gene="dkgA"
                     /locus_tag="ACHJ7J_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO5000081.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21141..22067
                     /gene="yqhG"
                     /locus_tag="ACHJ7J_001839"
     CDS             21141..22067
                     /gene="yqhG"
                     /locus_tag="ACHJ7J_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO5000082.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITETD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKAANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22118..22375
                     /gene="yqhH"
                     /locus_tag="ACHJ7J_001840"
     CDS             22118..22375
                     /gene="yqhH"
                     /locus_tag="ACHJ7J_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO5000083.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22418..24637)
                     /gene="ygiQ"
                     /locus_tag="ACHJ7J_001841"
     CDS             complement(22418..24637)
                     /gene="ygiQ"
                     /locus_tag="ACHJ7J_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO5000084.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKSDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSHPVNAGAKKRPKAAVGR"
     gene            complement(24748..26160)
                     /gene="ftsP"
                     /locus_tag="ACHJ7J_001842"
     CDS             complement(24748..26160)
                     /gene="ftsP"
                     /locus_tag="ACHJ7J_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO5000085.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26235..26972)
                     /gene="plsC"
                     /locus_tag="ACHJ7J_001843"
                     /gene_synonym="parF"
     CDS             complement(26235..26972)
                     /gene="plsC"
                     /locus_tag="ACHJ7J_001843"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO5000086.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27206..29464)
                     /gene="parC"
                     /locus_tag="ACHJ7J_001844"
     CDS             complement(27206..29464)
                     /gene="parC"
                     /locus_tag="ACHJ7J_001844"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO5000087.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29602..31209)
                     /gene="ygiS"
                     /locus_tag="ACHJ7J_001845"
     CDS             complement(29602..31209)
                     /gene="ygiS"
                     /locus_tag="ACHJ7J_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5000088.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEIMDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31318..31800)
                     /gene="ygiV"
                     /locus_tag="ACHJ7J_001846"
     CDS             complement(31318..31800)
                     /gene="ygiV"
                     /locus_tag="ACHJ7J_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO5000089.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(31853..32245)
                     /gene="ygiW"
                     /locus_tag="ACHJ7J_001847"
     CDS             complement(31853..32245)
                     /gene="ygiW"
                     /locus_tag="ACHJ7J_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO5000090.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32397..33056
                     /gene="qseB"
                     /locus_tag="ACHJ7J_001848"
     CDS             32397..33056
                     /gene="qseB"
                     /locus_tag="ACHJ7J_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO5000091.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33053..34402
                     /gene="qseC"
                     /locus_tag="ACHJ7J_001849"
     CDS             33053..34402
                     /gene="qseC"
                     /locus_tag="ACHJ7J_001849"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO5000092.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34512..35093
                     /gene="mdaB"
                     /locus_tag="ACHJ7J_001850"
     CDS             34512..35093
                     /gene="mdaB"
                     /locus_tag="ACHJ7J_001850"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO5000093.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35124..35438
                     /gene="ygiN"
                     /locus_tag="ACHJ7J_001851"
     CDS             35124..35438
                     /gene="ygiN"
                     /locus_tag="ACHJ7J_001851"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO5000094.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35483..36370)
                     /locus_tag="ACHJ7J_001852"
     CDS             complement(35483..36370)
                     /locus_tag="ACHJ7J_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5000095.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36367..37314)
                     /locus_tag="ACHJ7J_001853"
     CDS             complement(36367..37314)
                     /locus_tag="ACHJ7J_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5000096.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37325..38362)
                     /locus_tag="ACHJ7J_001854"
     CDS             complement(37325..38362)
                     /locus_tag="ACHJ7J_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5000097.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAT
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQCRNSF"
     gene            complement(38371..39354)
                     /locus_tag="ACHJ7J_001855"
     CDS             complement(38371..39354)
                     /locus_tag="ACHJ7J_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5000098.1"
                     /translation="MMRTGLVTVIFLTLLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPAGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWVTLGTSVWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39351..40160)
                     /locus_tag="ACHJ7J_001856"
     CDS             complement(39351..40160)
                     /locus_tag="ACHJ7J_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5000099.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT         9999 a        10832 c        10102 g         9325 t
ORIGIN      
        1 taatcagtac ggggttgcta caacatgtat tactttttca ccacaattaa cggttcaata
       61 tcactctctt ttttgatgac cagtgattca tcaccgcgca aacacgtccc accgtagttg
      121 ccgccaacgg tgaaggtaca tacctgaatg tatttaccgt ccactttcgg caaacaccac
      181 agttgctgat agatgttttt ctgctcggca aatttaccgc tggttttgtc cagcacctct
      241 tcatgatggc tgaccaggtc gatattgcta ccacagcgac cggcgatcgg tttcactgcg
      301 taacctgttt tcaccaattc atcattaaca gtgaaatcgg tatccagcag gtaacgatgg
      361 tgcgggaaca gcgaccagag gatcggcaga atcgctttgt tgccggggat caccgtccac
      421 agcggctcaa agaccagcac ttccgggcgc agcaatacgt cgataagacg cacttcgttt
      481 tgcggatgac cggtacggat tggcaccgca gcaaactcac ggtcgctaac ttcacgaatc
      541 tgatcaaacg cggtttccca cgcccaggtt ttccacacgc agttaaccag tcgcccttcc
      601 ccatcaatca gttgcccggc tgcatcccag cccagttcat ccagcccgcg caagatacgc
      661 gtttcaaagc ccgcctggtg cagcgcctgc tccataaact gcgcgtgata gttttcctcg
      721 atatctttgt cctgcatgat atggacaaac ggacgtgcac gactgtgttt ccaggcacca
      781 gccagttcgt taatcagccc ttccgccgga ttgaagccgt tgcctttata gccctgctcc
      841 gcccaacgtt cgaggatcaa gcccgcttcg gtatgacatg aggcggagtc ggcgttgtac
      901 tcgtaaacct tcagaccacg ctcatccatg cagaaatcca tacgaccggt gatcatatgg
      961 tgacggcgac gctgccagga gagacgcaaa cgtggccaga ggattttcgg aatgtcgaac
     1021 agcgccagca ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata catcaggtgc
     1081 agctcgttgg tggctttaat cagctcctgc tcggcgctct cggtaatggt gtagtaatga
     1141 tacggatcct gattgatcac ctgaccgttg gcctgcacat aggcgttttg cagcggatct
     1201 ttttcatcca gccatttacc gtcaaactgg cctttgtttt ccagacgcgc tccgctgatt
     1261 ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt attcagtatc ttccgtctgg
     1321 atcatccagc ccagaatggt ggtgtcatca aaagtgtctt taagggtata gcagccgttt
     1381 tcgaccacca tctccagttc gcgcgtccac tgttgccctt gcggcaacgg ggaatgaatc
     1441 acgttctgtt ccgcaatacg gactttgttg ccatgtaatt gggtaatgat ggcgacatgg
     1501 ccagtgtctt taaattcacc gcctttatcc cagataagaa gcgcacccgc gaccggcgca
     1561 cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac cacttcacgc
     1621 aggaagcgca gcgagaaaat ctcccacgcc atacccacgt cagtaaagac cacaccgtag
     1681 ttcagaaaga gaaaacggcg agcaaattca acgcattgcc atttgtggcc catatattcg
     1741 tcgtcgatat agctacggaa tacggcgtca tcttcgtatt cctgcggatc gagagaactg
     1801 taatctgaag agtagattgc taccccacct ggggcgtagc ccaataatgt cccgaacggg
     1861 gcatcctggc tggtcgttcc tttgctcatc actttacctt taacaataca acctaagcag
     1921 ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca ttaaccggac agaggtcaga
     1981 ctaatcatac acctcatcgc agcggctggc gcaaagggtt aaaaaattca cattctgtac
     2041 agcaagtgac ctgctacact gcttcaacac taccactcag aaggcaactc actatgacag
     2101 acaatactta tcagcccgcg aaagtctgga cgtgggataa atccgctggc ggcgcgttcg
     2161 ccaatatcaa tcgcccggtt tctggtccga cgcatgaaaa aacgctgccc gttggcaaac
     2221 atccattgca actttattcg ctgggaacgc cgaacggtca gaaagtaacg attatgcttg
     2281 aggagctgct ggcgctgggc gttactggtg cagagtacga cgcctggctg atacgcatag
     2341 gcgatggcga tcaattctcc agcggctttg tcgaagtgaa cccaaactcg aagatcccgg
     2401 cgctgcgcga tcatacgcat aatccgccga tccgcgtgtt tgagtctggt tcgatcctgc
     2461 tttatctggc agaaaaattt ggctacttct tgccgcagga tctggcaaaa cggactgaag
     2521 caatgaactg gctgttctgg ctacagggtg ccgcgccgtt cctcggcggt ggttttggtc
     2581 acttttacca ttacgcgccg gtgaaaatta agtacgccat caatcgcttt accatggaag
     2641 ccaaacgtct gctcgacgtg ctggataagc aactggcgca gcataagttt gttgcgggcg
     2701 atgagtacac cattgcggat atggcgattt ggccgtggtt tggcaatgtg gtattaggcg
     2761 gcgtatatga tgccgcagag tttcttgatg cgggcagtta taagcatgtg caacgctggg
     2821 caaaagaagt gggcgaacgt ccggcggtga aacgtgggcg tattgttaac cgcaccaacg
     2881 ggccgctgaa cgagcagttg catgagcgcc atgacgccag tgattttgag acaaatacag
     2941 aagataagcg tcaggggtaa gggttagcgt tcgtcgcagc aagccatcca ggccggataa
     3001 ggcgttcacg ccgcatccgg caatcgagca caatgcctga tgcgacgctg ccgcgtctta
     3061 tcagacctac gaaagctaat catcacgcgc tggtaatgtc gtaatccatt tggcgcagtg
     3121 cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat ccgacgtgta
     3181 ccagcaccca ctggcccagc agatcggcag ggttaccttc gcaaatcagg gcgatattca
     3241 cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct tcaccgacag
     3301 ccagcacctg gcctggaacg ccaatacaca ttgttaactc cggttatttc gattgccgga
     3361 caaacgcgaa aaagaacctt tagcgggtgt ttcccctcac cctaaccctc tccccggagg
     3421 ggcgagggga ccgtccgtgc gctttttagg cgttgtcctc agtcttattc aacttcaata
     3481 cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccatg acagagcgga
     3541 cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca ccaggcctgg
     3601 gcgggtttat agatgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
     3661 tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac
     3721 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gctggataat ttcaacggcg
     3781 ctctggcaaa gcgacaactc atgcattttc gccactcctg cgaccaaaca gcagggcgcg
     3841 acgccctgcg tgtggtacat ccggattggt gaccggcagc gaaaggatca ttcgtgcgca
     3901 gtcatccgtc aggcgttggc cctcttcaat cgacatactg tgcgaaagcg gtgacatcag
     3961 cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg tcatggtgcc
     4021 atacggcagt tgcagaccga ttttttcact cactttgcgc agcggccaga gttgatccgg
     4081 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
     4141 ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagatc
     4201 gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt
     4261 ctggaaacct gctatctctt cagtcatgaa tagcctcccg tgggatagct tccacgccag
     4321 attcacgcag cgcagccaga acctgctcaa gcgctggttc aatcatcgct tcgaccgtcg
     4381 gcgtcaagcc gatgtgcggc tccagtgatt ccggaattac gccgaccagg gtcagctttt
     4441 tcggaaactc gccggtgaag cgcagggccg acaagacgtc ggccaggcca agctgatgcg
     4501 gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc
     4561 ccggcgcgct cttcttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca
     4621 tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca
     4681 gaatgtatcg ttgctctaac gcttcgacaa tccgcacacc gatggcttca tcggtcagca
     4741 aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
     4801 gttgccgtca gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat
     4861 ggtacgcacc acttccagcg gtttattcgg atcggcaacc ggtgtaccca ccaacgactg
     4921 ctcgtaagga ccgacgtcat cattgaagtt acgcggacca gagttccagg ttgatggaac
     4981 aaccgcctgg tagttgctga tgataccgtc tttaataacc atccagtgag agagcatacc
     5041 gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggctt
     5101 cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attggttttg
     5161 caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc ccagcgtaga
     5221 gtgcagttgc gccacttcca gcgtgttacc agtcagtttc tgataaatcg caacgatttc
     5281 attcagtttg ttctgggtag attcgcgtcc tgccgccagt ttcactagca tattagccag
     5341 cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc
     5401 gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggtg cctgcggcgc
     5461 ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc
     5521 ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt agccgcccgg
     5581 gaacaggaag ctgccgtttt tactgtcggt cgggaattcc ggcacgctca ggtagttcac
     5641 cgcaccttta ccgcgcgtca gccattccgg gtagaacgca gcaataactg cggtatcaac
     5701 cttataaacc tgttcaacaa agtcgctcag cttgtcgatg aaagacttga tgtacatcag
     5761 gcgctcaagg ttcagcacgc ccaaaccgtc gaggttgatc gggttcgcga caccacctac
     5821 cgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc
     5881 acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg
     5941 cagtttcatc gccggatgac cccagtagcc gttggcgaaa atgcccaact gaccgctggc
     6001 aaccagatct ttgatcttgt tctgaacttt ggtgaactct tccggactgt tcagatgcca
     6061 ggtcgaaacg cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt
     6121 gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt
     6181 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
     6241 cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat
     6301 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
     6361 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
     6421 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
     6481 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
     6541 cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt
     6601 tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag
     6661 gtcagacgcc aggttgcaca acctaacagt gcgctcagtg ctgacaggaa cagcatgcgg
     6721 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
     6781 tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
     6841 tagataagct cgccaaagcg cagcacgatg aaaatcgcca gcaacacact gatggtgttg
     6901 gtcagcttga cgaacaggct cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc
     6961 agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
     7021 agcggcaaca tttcatagct ctgccacagc ggatgcacct tgtagcccgc cgagatcatc
     7081 agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag
     7141 aacatcacct tgttcagacg ctgtagcgac accttccagc ccagacgttc aaacagtgcc
     7201 ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
     7261 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
     7321 cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc
     7381 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgccagc
     7441 gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc
     7501 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
     7561 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
     7621 ttgccgccca gcggttttgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
     7681 tttggtgttg cgacgaacca gcacggttaa gcccgccagc acagccagtg gtagcatcat
     7741 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
     7801 tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc
     7861 gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc
     7921 tccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg
     7981 ttttttcgcc tccgccatca gctcttcacg cgtaccgaaa atcaccgcgc ccgccgggca
     8041 cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca
     8101 cagctcgcac ttatgtagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta
     8161 tgggcaggcg accatgcagt aacggcagcc ggtgcacacg tctttgtcgt aatggacaat
     8221 gccggttttc ggatcttttt tcagtgcaga aaccgggcac acagagacac agttcggatc
     8281 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
     8341 gacccctgtg ccgctggtcc acacctgaat gatgttattg gtatacggcg acagtttgtc
     8401 gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga tatcctgaca
     8461 cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga
     8521 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt
     8581 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
     8641 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
     8701 acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg
     8761 ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt ttcgacgttg
     8821 gcaagctgat ggatgccttt atggaagccg ataccttctt cgttacagcc ataacacggg
     8881 tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg
     8941 ccgtaagttt ctggcccttt acagccgagg tggtacaggc accagccttc gcggtggcct
     9001 tcatcaccga actctttggc aaaacgaccc gcatcgaagt gcgggcgacg ttcgcagtgt
     9061 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt
     9121 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
     9181 cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac tgcgccagtt
     9241 gggttaactc cagctgcggc aacaccgccc cacgcagagc aggaaccgat agcgataatg
     9301 gctgctgcgc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
     9361 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
     9421 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa
     9481 agcacttcgt gatactccag agagatagtc tccagtacga ggttttctac cgttggatgc
     9541 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
     9601 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
     9661 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
     9721 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgaa gacctggcat
     9781 atattttgcc tcaatcgcaa aatcgataat gcgatcgatg cgccatttac cacacattta
     9841 ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta
     9901 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg
     9961 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
    10021 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
    10081 ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    10141 cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    10201 agcctttacc aaaatggtta ttcatcgttc acctcattcg ctatatatcg tcatatatag
    10261 cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatgctt gtttaagacg
    10321 taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt
    10381 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    10441 tgatttaccc ccggatagat atacgcctca taaaccttat tattggcttt caacgccgcc
    10501 tcgtaagcag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa
    10561 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcaccgcg
    10621 caggccagtt ccggatacgc gacagccgcc gcgttcgata cgccaccgcc atagcaaaat
    10681 ccggtaatac ccactttgcc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    10741 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    10801 tcatttcccg gataacctcc aacggaactt aagccgtcag gtgccagggc gatatacccc
    10861 gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc
    10921 accaccacca cggctggcgt tttgccgctc atttttgcgg gtttcaccag ataaccccgt
    10981 acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaacaat ctccgggtcg
    11041 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat
    11101 gccgtcatcc cgcccaccgc atacttcgcc gcaaggttga ggaactcacg tttcgagatt
    11161 ttcccgtgag cgtaatagtc gtagtaatcc aacaactctt gtgggaaatc tttggcggtt
    11221 aaacgcggca tcgttgcact cctcagttgg tgttttttta agcaaagcat aagcacgtgt
    11281 ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc
    11341 taaactagaa acattgtttc gaaattgaac ggtggaaagg agaggtcatg gtctggttag
    11401 cgaatcccga acgttacggg cagatgcaat accgctattg cggaaaaagc ggtttacgcc
    11461 tgcccgcgtt atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac
    11521 agcgtgcgat cctgcgtaaa gcgtttgatt tgggcattac gcactttgat ttagccaaca
    11581 attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg cgggaggatt
    11641 ttgccgctta tcgcgatgaa ctgattatct ctaccaaggc tggctacgat atgtggcccg
    11701 gcccttacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga
    11761 agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc
    11821 cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg
    11881 tcgggatatc ctcttactcg ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt
    11941 ggaaaattcc gcttttaatt catcaacctt cgtacaattt actaaaccgc tgggtggata
    12001 aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgcattgcc tttactcctc
    12061 tggctcaggg attgctgacc ggaaaatatc tcaacggcat tcctgaagat tcacggatgc
    12121 atcgtgaagg gaataaagtt cgtggcctga cgccgaaaat gctcaccgaa gccaacctca
    12181 acagcctgcg cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg
    12241 cgttaagctg gttgctgaaa gatgatcgcg tgacgtcggt attgattggt gccagccgcg
    12301 cggagcaact agaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc
    12361 tggcgcagat tgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg
    12421 ataaatgacc tgttaattac gggcgcgaga accgcgcccg aatcagatca gtgattatga
    12481 cgagtcagtc ggtcaagata gcccatcaca aatgcagaga gcacaaacgt cagatggata
    12541 atgacgtacc acatcagttt gttatcaggg acatttttcg catccataaa gacgcgcagt
    12601 aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgca
    12661 tccattttcc ccagccagtt cagcttctct ttgttctcgg agatatccag ttgtgagacg
    12721 aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc
    12781 accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt
    12841 acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca
    12901 aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc
    12961 atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcgc
    13021 gcaacagcaa atcaacatat ccgttaaatc tttgtcgcat aattctgagg gttagggatc
    13081 ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa
    13141 gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat
    13201 cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccggt accggattgc
    13261 ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagccct ggtgacaggg
    13321 ggcgattccg gaatcggtcg cgctgccgcc atcgcttacg cacgtgaagg ggctgacgtg
    13381 gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg gtgtgaaaaa gatcattgaa
    13441 gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa atttgcccgt
    13501 tcgctggttc acgaagcgca taaggcctta ggcgggctgg atattatggc gctggtcgcc
    13561 gggaaacagg ttgccattcc ggatattgca gacctcacca gcgaacagtt tcaaaagacc
    13621 tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg
    13681 aaaggtgcaa gtatcatcac cacttcgtca atccaggcat accagccaag tccgcattta
    13741 ctggactatg cggctacgaa ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag
    13801 gtcgcagaga aaggtattcg ggtgaatatt gtcgcgccag gcccgatctg gacagcactg
    13861 caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagaa aacgccgatg
    13921 aagcgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag
    13981 tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa
    14041 aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc
    14101 gaaaacgcct tatccgccta caaacgcatg aagattcaac atgttgcaat agattttgta
    14161 ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg gcgtgaacgc
    14221 cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga
    14281 tgagcgtagc gcatcaggca attctacttt acttcgcttt agcggtttct tcgccgacca
    14341 gacctatctt caggtaaccc gcctgatgca gcgtgtccat taccttcatc aacgtctcgt
    14401 aatcgacagt tttatccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg
    14461 cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt
    14521 tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag
    14581 aagcaggcaa gttaaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa
    14641 tcagcagaac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt
    14701 tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgca cgtaattttt
    14761 gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca
    14821 gcaacaatac ctgcgctgcg acatcaccca gcatcgcttt aaagccgcca atctgacgcg
    14881 cgaatacgtt atagataacg accgccggaa tcgctgccac cagaccgatt gccgttgcta
    14941 acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtagtctgc gtttgcgcga
    15001 taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagcccaacg aacggcgaaa
    15061 tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgacct actgcggcga
    15121 cccgacgctc cagacgaaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt
    15181 ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac
    15241 caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg cgcttcagcc agcagttgct
    15301 gctcgcgctt aaggcgacgc ttctgattga agaactctac gctcttactg aagaagattg
    15361 cccaggtgac tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg
    15421 cgtgctgata cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt
    15481 atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga
    15541 tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta
    15601 cgcagtaaaa aaatcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt
    15661 caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat
    15721 cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa
    15781 tacactctcg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt
    15841 atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg
    15901 cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca
    15961 ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc
    16021 gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt
    16081 agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc
    16141 gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccgggttcc
    16201 atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg
    16261 gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat
    16321 tttggcatcg atgtttctat tcaggccgcc accaaatatc tggttgggca ttcagatgcg
    16381 atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat
    16441 ctgatgggcc aaatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca
    16501 ttaggtgtgc gtttgcgtca acatcatgaa agcagtctaa aagtggctga atggctggca
    16561 gaacatccgc aagttgcgcg agttaaccac cctgctctgc ctggcagtaa aggccacgaa
    16621 ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa
    16681 ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag catggcctac
    16741 tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgccgccatt
    16801 cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa
    16861 gatgtcgacg atctgattgc cgatctggac gccggttttg cgcggattgt ataacattgc
    16921 cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg
    16981 atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc
    17041 cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt
    17101 tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct
    17161 gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatact
    17221 ggcaggcgca ttgattgccc agggggttat ggattttctg cctacgattg cgattctgac
    17281 cgccgcagca agtctgggct gctggctaag ttatattcag gggcgctggt tagggaatac
    17341 caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcatcagc gcgccacctg
    17401 catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg
    17461 tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgtt tccagttttt
    17521 caactggtta agcggattgc tgtgggtcag cgtggtaacc agtttcggct atgccttaag
    17581 tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc
    17641 aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata
    17701 ctgtaacgcc tgacgatttt ccctgttccc ggttgctgta ccgggaacgt atttaattcc
    17761 cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa
    17821 actcacggct aaattgcgat gcgctttcat agccaacgcg catcgctgct gcgctggcct
    17881 tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact
    17941 gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatat
    18001 tggcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaatccgtt
    18061 tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc
    18121 cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
    18181 tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg cataaaatct
    18241 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat
    18301 cttcgccaat gtccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc
    18361 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
    18421 gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat
    18481 gtccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg
    18541 tctcgccata caacaaacgc acatcgggca acagttctga caaactattt tctttatttt
    18601 tcaggttatt aactttattc gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta
    18661 atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac
    18721 tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat
    18781 cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc
    18841 aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa
    18901 tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggtggcg
    18961 gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg
    19021 tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga
    19081 aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt tctgtactgg
    19141 acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca
    19201 ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc
    19261 tgccagcaac cggttcagaa tctaacgccg gtgcggtgat ctcccgtaaa accacaggcg
    19321 ataagcaggc gttccattct gcccatgttc agcccgtatt tgccgtgctc gatccggttt
    19381 atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca
    19441 ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag
    19501 gcattttgct gacgctgatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg
    19561 atgtgcgcgc caacgtcatg tgggcggcga cgcaggcgct gaacggtttg attggcgctg
    19621 gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc
    19681 tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata
    19741 ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg
    19801 acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg
    19861 tgcctaccca cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa
    19921 aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg ctggatgtca
    19981 gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg
    20041 catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat
    20101 aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc
    20161 ccagttggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa
    20221 agcgttagaa gtgggttatc gctcgattga taccgccgcg gcctataaga acgaagaagg
    20281 tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca tcactactaa
    20341 gctgtggaac gacgaccaca agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact
    20401 ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta
    20461 tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg
    20521 cgtgtgcaac ttccagatcc atcacctgca acgcttgatt gatgaaactg gcgtgacgcc
    20581 tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcctggaa
    20641 cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt
    20701 tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat
    20761 tgttatccgt tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc
    20821 acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcga
    20881 aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta
    20941 acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta
    21001 ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac
    21061 atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc
    21121 aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt
    21181 ttaccgtaca cgcacagccc ccctcacaga ccgtagaaca aacagtccgg catatttatc
    21241 agaactataa atcagatgcc actgcccctt attttggtga aaccggagag cgggcgataa
    21301 cttctgcgcg tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggcaata
    21361 ttggctggct ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgctag
    21421 aaagcgtagc gataaccgaa actgacgccg atcatgccga tgccgttgtg cgctttcgta
    21481 tctttaaaga tgataaagaa aagaccacgc agacactgaa aatggtggcg gaaaatggtc
    21541 gttgggtcat tgacgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg
    21601 agaatgaaaa aacgctggcc gctttagctt cgttgcaaaa agaacagccg gaagcctttg
    21661 ttgccgaact ctttgaacat attgccgatt atagctggcc gtggacgtgg gtggtttccg
    21721 actcttaccg ccaggcggtc aatgccttct ataaaaccac cttcaaggcg gccaacaatc
    21781 ccgatgaaga tatgcaaata gaacggcaat ttatttacga caatccgatc tgttttggcg
    21841 aagagtcgct attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg
    21901 cccgcattca tgttcgtttt acgctgacca atggcaacaa cgaagagcaa gaactggttt
    21961 tacagcggcg cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc
    22021 tacttaagca gattgaggca aaaaccgccg ccagattaaa gcaatgagct gaattaaata
    22081 acaattagcc ggaacaataa ataaaaggga acactatatg aaaacgattt tcaccgtggg
    22141 agctgttgtt ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca
    22201 gctggcgagc aatgtgcaaa cattaaatgc caaaatcgcc cggcttgagc aggatatgaa
    22261 agcactacgc ccacaaatct atgctgccaa atccgaagct aacagagcca atacgcgtct
    22321 tgatgcacag gactattttg attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa
    22381 aaatccccgg cagcatgtca gttgccgggg atatttttta acgtccaacc gccgctttag
    22441 ggcgtttctt cgccccagca ttcaccggat gagattgcgt agacgacgct ttttttgccg
    22501 tagcaggcgt ctgacgctgg gtcgccatcg gcgtatgttt cgtcaacgct ggacgagtat
    22561 tgcggttctg acgacgagcc tcacgcatct cttcaatggt tggcgcaggc actaagcaat
    22621 cgcgacggct gccaatcaga tgctttttac ccatcgcttc cagcgcctgg cggattaacg
    22681 gccagtttgc cggatcgtgg taacgcaaca acgctttatg caaacgacgc tgcttgtcgc
    22741 ccttcggtac gaagacgtct tcgctcttat aaccaatctt cgccagcggg tttttcccgg
    22801 tgtaatacat ggtggttgag ttcgccaacg gcgacggata gaagttctgc acctggtcga
    22861 ggcggaagcg gtgctttttc agccacagcg ccagattcac catatcttca tcacgcgtac
    22921 ccgggtgcgc ggagatgaaa taggggatca gatactgctc tttacctgcc tgtttcgagt
    22981 aggtatcgaa cagctcttta aagcggtcat aactgcccat gcccggcttc atcatcttcg
    23041 acaacggccc ttcttcggta tgttccggag caatcttcag ataaccgccg acgtgatggg
    23101 tcgccagttc tttgatatag cgcggatctt ccacggcgat gtcataacgt acgcctgagg
    23161 caatcaggat ttttttaatg cctttcagat cgcgagcgcg gcgatagagg ttgatcgtcg
    23221 gttcgtggtt agtgtccatg tgcggacaaa tatccgggta aacgcaggac aaacggcggc
    23281 aggtttgctc agcgcgtggc gatttgcagc gcaacatata catgttggca gttggcccac
    23341 cgagatcgga aattacgccc gtaaaaccag gaacggtgtc gcggatcgct tcgatctcat
    23401 taatgatcga atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg gtgatagaac
    23461 agaaagagca gccgccaaag cagccacgca taatgttgac cgagaaacgg atcatttcgt
    23521 aagccggaat acgggcatta ccataggccg gatgcgggac acgtttgtac ggcagcgcaa
    23581 agacgctatc catctcttcg gtagaaagcg gtattgcagg tgggttgatc cacacatagc
    23641 ggtcgccgtg tttttgcatc aacgcacggg cgcagcctgg gttagtttcg tggtgcagaa
    23701 tacgcgaagc atgagcgtac aggactttgt cgcttttcac tttctcgaat gaaggcaaca
    23761 acacgtaggt tttttcccac ggtttcgggc gcggcggctg cacggtaacg gctttggctt
    23821 cctgtttttt cggtgccacc ggtttgttat cagcgcacgg caggtcttca ccatatgggt
    23881 gcgggatcgg gtctattttc cctggggtat cgagacgggt ggaatccacg ccgctccagc
    23941 caggcagcgc ctctttcacg ataatcgcgg tattacgcac atcgcggatt tcactaatcg
    24001 gctcgcccat cgccagacgg tgcgccacct ccaccagcgg acgctcaccg ttaccaaaca
    24061 tcagcatgtc ggctttcgaa tccaccagca cggaacggcg cacggtatcg gaccagtaat
    24121 cataatgtgc ggtacggcgc agactagcct caataccgcc gaggatcacc ggtacatctt
    24181 tccacgcctc tttacaacgc tgggtataaa ccagtgtggc gcgatccggg cgcttacccg
    24241 cgacgttatc cggcgtgtag gcatcgtcat gacgtaaacg gcgatcggcg gtatagcggt
    24301 tgatcatcga atccatgttg ccagcagtaa caccgaaaaa cagattcggt ttacccagac
    24361 gcataaagtc gtctttgctg ctccagtctg gctgagcgat gatcccaacg cgaaagccct
    24421 gtgcttccag catacgaccg caaatcgcca tcccgaagct tgggtgatcg acatacgcgt
    24481 cgccagtaac caaaatgatg tcgcagctat cccagccaag ttgatccatc tcttcacgag
    24541 acatcggcaa aaacggtgcc ggtccaaaac aggcggccca gtactgcggc caggagaaca
    24601 ggtcgcgatc cggttggatc agggagatag agctcatttt gcttccagaa atgataaaaa
    24661 aataatcaaa ggccggggat tataagccgg aacgaaagag aaatcgaaaa gtattccata
    24721 ctcgccctcc tcgggcgagt atgaagatta cggtaccgga ttgactaaca gttgcccaat
    24781 cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga agtgcgccca
    24841 ggaaggctga ccgaaataaa caagcagctc cacttgtccg tcaacccaaa cggtatcttt
    24901 ccagcctctg tcttccggga acggcatcgc accattcacg ttacggatct ggaacatcac
    24961 gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt cccacgttcc
    25021 ttgctgcgcg gtgacatcaa tacggttgac gtcccacagt tgtccattaa tacccgggtc
    25081 atcacccaga ctgatatcgc gactgcgaat cggcgaaccg gccatgattt cagttggcag
    25141 caagcgcatc ggaagactgt ctgtgaccag cggcagaagg ccggttgggc gcagcgttag
    25201 caccagggta gaaaccagaa tgctggatgg ctcaaagaag ccacgaatac gatcaacaat
    25261 gctcgccgcc tcgccacagg tgatcgacac ttcatcaccg ttgctcatat ccaccagaat
    25321 ctcgcggcgc tcgcccggtg ccaacgaaag ttgcttcacc gatacaggag caggcaggaa
    25381 tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta gttgatagcg
    25441 acgagagttc gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga cttcaacgta
    25501 cgggctttgt acaccgttaa ccagcagcgt atcaccaaca aagccgccgc ttcccggttc
    25561 gttgtattct ggcgtaccaa agttatccag ccgtttatcc tggataatga ccggaaaatc
    25621 atccacacca taatggttgg ggataggcag cgacttgctg acttcatctt ccaccagcca
    25681 cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat tggcgtgata
    25741 ccacagagta gctgcgttct ggcgaatggg cagtacgggt gcccagtcag cgtttggcga
    25801 catcatccgt gccggaccgc ccatcagcgg gcctggtacc tgtagcccgg ccaccgtcat
    25861 tgagacattt tctgtcaggc ggttgctgta aataagctta acatcgtcgc ccttccagac
    25921 gcggatagtc ggccccaggt aacgaccatt gattccccag accgacgcgc gtgtccctgg
    25981 cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac ggcgagattc
    26041 aagcagcggc ggaacgggta gcggttgctg ttgcccggct gcgctggcct tcaggggaac
    26101 agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac tgagtgacat
    26161 atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa
    26221 tacagattgc cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc tttatcgagc
    26281 tcggcgattt tttgttccat tatcgaacga caatgggcag ccaactcacg aacctgatct
    26341 ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag accgttgtgc
    26401 agtcgattaa gattaatctt attcgaagtt gtagagacgc acacgggaat aatcgggacg
    26461 cctgctgcaa ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc gcgaccacgg
    26521 ctgcgggttc cttccgggaa catccaaatg gaaatacggc gttttttgaa atgattcact
    26581 acttccgcaa tagtgccgtg agctttagtg cgattgtttc tgtcgatcaa taagttgccg
    26641 gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt acctaccgtc
    26701 accgtcggcg gttgcacaat gttcgatgct gtcaccatgt catagttgtt ctggtggtta
    26761 gcgatataga tagcattgcc gtagctttca gcatctgcag gtttgcggca ctcaactttc
    26821 aggccgaaca gcggcgcaag acgaccaaac atatgcccaa aggtggccac atgtttcggg
    26881 ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat gctgtaaatc
    26941 acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct gagagactgg
    27001 cattgtacgt cacctgaaat caggttaggg cattgttaaa agccgctctc tggaaaagcg
    27061 tattgttaat cgcaacgcat gaataaacaa cggtttcaag ggaattttta tcgtttccgg
    27121 gggttgaata cgctgccgga tgacgactta acgtttcatc cggcgttcct tgcaagcggg
    27181 aggaaacagc gccctccccg gcatattact cttcgctatc accgctgctg gcacggcgag
    27241 gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg catcaacgta ccgcggcgtc
    27301 cacgttcgcc agtgactttc tgtagctctt ccggacgcag tttaattttg cgtttcccaa
    27361 catgaatggt cagcgtgctt tgcggcggca gaacgtacag ttgcgccaga ccatcctcgc
    27421 cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc ttcgacagct
    27481 gcggcagatc acttaccggg aacatcaaca tacggcctgc ctgagtgatt gccagcagca
    27541 tgtcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc ggtaaggtta
    27601 tcaaagcctt acctgcacgg ttacgcgcca ccagatcgtt aaaggtgcag acgaaaccgt
    27661 aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcagc atatggtcaa
    27721 cggtcgcccc aggcggcaac gttaatttgc cggtgagcgg ctcgccctga ccacgcgccg
    27781 acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca aacactaccg
    27841 gttggttgct cttacctttc actgccgctt tgaagctatc acccgcttta taattcaggc
    27901 ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca gcccatctgc gacagcacaa
    27961 tggtgacagg ttcagacggc agcatgtcgt gctcgctcat cgctttcgct tcttcgcgtt
    28021 cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc gtctgcctgc agttctttct
    28081 tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg tcgcgctctt
    28141 tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca agatgacgca
    28201 gtttcagttc gaggatcgct tccgcctggg tttccgtaag gccaaaccgc gacatcagcg
    28261 ccggtttcgg ttcatcttca ttacgaatga tctcaatcac ttcgtcgata ttgagaaacg
    28321 ccaccagcaa accttcgagg atatgcaggc gcttgaggac tttctccaga cgatagttca
    28381 gtcggcggcg cacggtatcg cggcggaaca ccagccattc ggagaggatt tccagcaggt
    28441 ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga tagctctttt
    28501 ccagatcggt ggtagcgaag aggtggttca tcacctgatc catatccacg cggttggaac
    28561 gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc agatcgtcaa
    28621 ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg cgcgcacctg
    28681 aaacctgatg cggcaatgcg ctgataacca ccgcgccatc ttctttcttc cacaccgcgc
    28741 gcatacgcac tgaaccacgt ccgttctcgt agattttacg gatctcggcg cgcgaagtga
    28801 taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc tgatcgagcg
    28861 tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc cacttcacgc aggttatgcg
    28921 gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc aaaatgtttg
    28981 gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc gtcgaagttt ggcacccagt
    29041 cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac aaccgggatt
    29101 cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag ttcccctgac
    29161 catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc gcttcataac
    29221 aggcgctatc gccgtgcgga tggtatttac ccagtacgtc accgacggta cgggccgatt
    29281 ttttaaattt ggcgctggca ttcaggccca gttcagacat cgcatacaca atgcggcgct
    29341 gaacaggttt cagaccatca ccaataaacg gcaacgcacg gtccatgatc acgtacatgg
    29401 agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct gccatatcgc
    29461 tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata ttgccggaga
    29521 tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc cgccggacgt
    29581 gctccgacgg cgataaaggc atcaatgtgc cttgatatac aactctttgc tgtagacata
    29641 atcctgggga ttatgcagcg gaaaaccgcc aacgtagggt ttaagcagtt tgattaacgg
    29701 ctgataatag atgggaatca gcggtgcctg ttggttgatg atcacttctg cctgctgata
    29761 caacgcatta cgctttgtcg catcagtgat ctgcgtggcc tggtttagta aggcgtcata
    29821 ctgcgcattt ttccagtgac cgacgttttc ttcactatcg cttttgagcg tgttcaggaa
    29881 gctggaagca tcattgtacg tcgcatccca cgactgccga gacagcatga aatcaccggc
    29941 tcgtcgggca tcaagatagg ttttccactc cattgtgcgc agcgtcacct gtgcacccag
    30001 ccattttttc cattcggaag acaacgctat cgcggtcttt tcatgcagat cgtacttgtt
    30061 gtagaacagc tcaaagcgta gcggatgaga ggcgtcgtat cccgcctgtt tcagcaaggc
    30121 ttttgccatc gcgacgcgct cactcattgg cttttgcagt tcatcgaacg tcgtcgcgct
    30181 aaagcctttt acctctggcg gcgtcagcgt ggttgcgggc gtcctcaacc ccagtacctt
    30241 ttgcgcaata agctgtcgat caaccgtaag atatagcgcc cgacgcactc gcacatcgtt
    30301 aaatggcggt ttctcaaggt tgaagttgta atattcgctg ttcagacgcg gaataattcg
    30361 tagctctcca ggcagtgatt tttcaatggc gggaatttgc tgcgccggaa cccaggtgag
    30421 atcgacctct cccgcgcgat agcggttata gccggtgacc gaattatcca gcgccagata
    30481 ctcaacctgt tgcaatactg tatgttgcgc atcgcggtac tttggatttt tgcgtgcagt
    30541 aatcttttcg ttaactaccc actgatcaag cacaaaggca ccgttgtaaa ccatgttctc
    30601 tggcttactc cagctatcgc catgtttagc gatgacatga tgaggaaccg ggaacagcgt
    30661 cggccaggcg agcatcgtcg tgaaccaagg aaccggctgc tcaagcgtaa cttcaagagt
    30721 acgatcatcc gtcgctttga cacccagcga tgtaacatct gctttacccg caacaatagc
    30781 cgcggcattg ttaatgtgcg cctgtgccag atagccagca aaagggcttg ccgttttcgg
    30841 gtcaaccgcg cgctgccagc cgaggacaaa atcctctgcc gtcagaggct gaccgtcaga
    30901 ccactgcaaa ccgctacgca gatggaaaat atagcgcctg ccgccgtcca ttatctccca
    30961 gcgttcagcc tgagcgggct gcacctggcc ttcaccgtcc atccatacca gaccttcaaa
    31021 cagatccagc acaatctgcg cggcagtatt ctcctccacc ttttgcgggt cgagcgtacc
    31081 tgggtcgcta tgattgttgt aacgaaagac ttgttgcgga gcgagcggtg tgttggtggg
    31141 aacgtcagca gcgtagagag gagccgcact taccaggctg accagccata ataaatttcg
    31201 cgtatacatg gtattcctta ccggagaaat tataagccca acaacaatac gcctgtggca
    31261 ttgttcgctc aaacttatca cgagtgattt ggctcacact ccggtaaaga ttccccatca
    31321 cgctaacggc acataaacat ccgtttccag tcgctgctct gtcacccctt cggcaagatt
    31381 ggtgtagtga aacagaatcg gtgctttacg cattttctcg ccgcttgcag gcagccagtg
    31441 gcgaataatg ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac
    31501 ggcataacgt ccaccggtaa gttcaccatt gctcacacca taacgattat cgggaatcgg
    31561 ttcgctaacg ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg tggcagggtc
    31621 gtcccaggcg acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg
    31681 ccacatgata aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa
    31741 catcgccaca gggattgatg ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat
    31801 tttgcctccc tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac aattacggat
    31861 ttactttgcg gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac
    31921 cctgaatctc aaccgtatct ttcggcgtca ccgtcacgcc gttccagcgt ttgtgatcga
    31981 tatcaacatt gatagtaccg ctggcatctt tgaacacgta gagatcgtca gagatgcgtt
    32041 caacgatatt gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta
    32101 cagtcgttac gctgccgttc ggcccctgga atcctccggc ctgactttgc gttgccgatg
    32161 ggccagaaaa accgccctgc tctgctgcca tcaccggtgc gctgcacagg gccattactg
    32221 cgattactgc tgcgaatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat
    32281 ttaacaagat agtccttaac aacttcttaa gggaaaaaaa taaaatttag tgctgtacag
    32341 agcgcgttac aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc
    32401 gaattttact gatagaagat gacatgctga ttggcgacgg catcaaaacg ggccttagta
    32461 aaatgggttt tagcgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctttata
    32521 gcgcacctta tgatgcggtg atcctggatt taaccttacc aggcatggat ggtcgcgata
    32581 ttttgcgcga atggcgagaa aaaggtcagc gtgagccggt actgatcctg accgcgcgcg
    32641 atgcgctgga ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac
    32701 cttttgcgtt gatagaagtc gccgccaggc tggaagctct gatgcgccga accaacggcc
    32761 aggccagcaa cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca
    32821 cgctggctgg cgaaccctta acactgaaac caaaagaatt tgccctgctg gaattactga
    32881 tgcgtaacgc tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg
    32941 acgaagaggt caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg
    33001 gtagtgattt tattcgtacc gtgcatggta ttggctacac attaggtgag aaatgaaatt
    33061 tacccaacgt cttagtctgc gcgtcaggct gacgctaatc tttttaattc tggcctcggt
    33121 gacctggctg ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt
    33181 gttcgacacc caactgatgc tgtttgccaa gcggttaagt acgctcgatc tcaacgaaat
    33241 caacgcggcg gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga
    33301 cgatgcgctg acctttgcca tctttaccca cgacggcaga atggtcctta acgatggcga
    33361 taacggagaa gatattccct atagctatca acgggaaggt tttgctgacg ggcaactggt
    33421 gggcgataaa gatcaatggc gttttgtctg gatgacctca cctgatggca aatatcgcat
    33481 cgttgttggt caggaatggg aataccgtga agacatggcg ctggcgattg ttgccgggca
    33541 attgatcccg tggctggtcg cactgccgat tatgttaatc atcatgatgg tactactggg
    33601 tcgtgaactc gcgccgctga acaagctggc gctggcgcta cgtatgcgtg accctgactc
    33661 ggaaaaacca ctaaacgcga ctggcgtacc cagcgaagtg cgtccactgg ttgagtcgtt
    33721 gaatcaactg ttcgcccgca cacatgcgat gatggttcgt gaacgacgct ttacctctga
    33781 cgcagctcac gaacttcgta gcccgttaac ggcgctgaaa gtgcaaaccg aagttgcgca
    33841 gctctctgac gatgatccgc aggcgcggaa aaaagcactg ctccaattac attctgggat
    33901 cgatcgcgct actcgtctgg ttgatcaact gctcacgcta tcgcggctgg actcactgga
    33961 taaccttcag gacgtcgcgg agatcccgct tgaagatctc ctgcaatcgt cggtgatgga
    34021 tatttaccac acggcgcagc aggcgaaaat tgacgtgcga ctgacactca atgtccaggg
    34081 catcaaacgc accggacaac cgctattgct aagtttgttg gtgcgaaatt tgctggataa
    34141 cgccgtgcgt tacagtccac agggcagcgt ggtggacgtc acgctgaatg ccgataattt
    34201 catcgtgagg gataacggcc ccggtgtgac accagaggca ctggcgcgaa ttggcgaacg
    34261 cttctatcgc ccacccggac aaaccgctac cggcagtgga cttgggctat cgattgtcca
    34321 gcgaatcgcc aagctgcatg gcatgaatgt tgagtttggg aatgcggaac aaggtggatt
    34381 tgaggcgaag gtaagctggt aagggtaaga cttttgctaa attcgcaaaa gactttgcac
    34441 attttgctaa tttcaccgta ccgctctgtg acgtactata gtcggcaaac gtctcacctt
    34501 gaggttaaaa aatgagcaac atcctgatta tcaacggcgc gaaaaaattc gcccactcca
    34561 atggtcaact gaacgacacc ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc
    34621 atgatgttcg catcgttcgc gccgacagcg actacgatgt caaagcggaa gtacaaaact
    34681 ttctctgggc tgatgtggtg atctggcaga tgccaggctg gtggatgggc gcgccgtgga
    34741 cagtgaaaaa atacattgat gatgtattca ccgaaggtca cgggactctg tatgccagcg
    34801 atggtcgtac ccgcaaagat ccgtcgaaaa aatacggttc cggcggcctg gtacagggaa
    34861 aaaaatatat gctttctctg acctggaacg caccaatgga agccttcacc gaaaaagatc
    34921 agttcttcca cggcgttggc gttgacggtg tgtatctgcc gttccataaa gcaaaccaat
    34981 tcctcggtat ggaaccgctg ccgacattta tcgctaatga cgtgataaaa atgcctgatg
    35041 ttccccgcta tactgaagaa tatcgcaagc atcttgtgga aatttttggt taactagagc
    35101 tcaggcttta gaaggagtta accatgctta ccgtaatcgc agaaatccgt actcgtcctg
    35161 gtcaacatca ccgtcaggcg gtattggatc agtttgctaa aatcgttcca accgtactga
    35221 aagaagaagg ttgccacggc tatgcgccaa tggtggattg cgctgctggc gtgagtttcc
    35281 agtctatggc accggactct atcgtgatga ttgagcagtg ggaaagcatc gcgcaccttg
    35341 aagcgcatct gcaaaccccg cacatgaagg cgtacagcga agccgtaaaa ggcgacgtgc
    35401 tggagatgaa tatccgtatt ctgcaaccgg ggatttaaca ttcctgaaaa gaggccggtg
    35461 ttgctaccgg cctcaggtca gattagccat tctttcttcc cgttttcagc ccccgatgga
    35521 aatcgttaat tcgcttaatg gttttaaccg cacgctgcgg caccgcggaa gccaccgacc
    35581 agacaatcat ttccaggcac aacaataccg taccatgtag tggcgttttc cccttttcat
    35641 ccccgcgcgg aacatgaatc acgatactgg cgtctttgct aaaacgtgaa tccagcgcgt
    35701 ttgtcagcaa aataacaggg atccccagcc gtctcgcttc acgcagcgtt gtcagccctt
    35761 cccggtgcgc agatttttgc gccatcatga tcagaacatc gccacgttga agcgcgatca
    35821 gttgctcggc aaggccaata cccgtacggt ttagtgccgt ggctggcaaa cctatgcggt
    35881 tgaatagtcg tgcggtatag tccgccagaa tacccgaagc gccaatgcca aaaattgcga
    35941 cttgccgtgc ctgtaccaac aacgctaccg cttgcgcaag cgcgtggcgg ttttcaggct
    36001 ctgacaggac attacaggta tacaaatgcc cttcaagaac aaagtcgatt gcggtgttaa
    36061 catcgctcgt caagttactc accgtcgtag acatcttttc gctggaacta agggctggac
    36121 caagccactg ttccagagtg cgtttcagat cacgcagccc ggcaaacccc agggcctgta
    36181 tagccctgat aactgtggca tcggaagtgt tcaacgtggc cgcaatttcc atcgccgttt
    36241 gctcgagtac cacttcacga tgctcattaa tatagctggc gacagctctt aacccaggcg
    36301 ttaactgact cgcgcgagtg cgaaaacgct caccataaat atcaacccgc gttttgtctt
    36361 tctgaatcat ttggcctccg gtaaaggtcg ccccgcaatt tgtgattgta tctgcgcagc
    36421 catcagcccc agagatgcaa aggagttaga aattgcctct tcacgagaaa tcagcacata
    36481 acgcccggaa cgacaggcag tcagaaactg acaccatccc ggcatgactt tttccattgc
    36541 tgccagctca tcctgaggtt taccaccggt gtcaccgcgc caggttgcaa aaacaaaatc
    36601 ggcatccagt tcaggtaaac gctccgcgct cacatccatt cgcccacctt caggaatgct
    36661 ctcaatcagc ggcggaaagc gaaaaccagc atcgcgtaat acccgcccca gcgaatgata
    36721 gctatgcatc acattaattt ttccctgatt tgcctgaatc accgataccg ttattttttg
    36781 gctgtccagc gtcgccttca gcgcattgat ttgtgcctga tagcggcgtt caagtatcgc
    36841 cagctgcgac tgcgttcctg tcagttctgc cagcttgcgg tagatttctg gcgcgcctcc
    36901 tttgagatga tcaatgctca ccgtgggcgc aattttttcc agttgttcaa tgggcgtatt
    36961 acgggtcggt tctgtgataa tcaggtcagg ctttgccgcc acaatcgctt caatatcaat
    37021 atcagcggtg ccaataaaag cgatcgatga attatcaaag tcgacacctg tcaataacgc
    37081 gcctgatcga atgaaatggc taccgtcagg ccgagttcgc ccgtggctgg cgacaggagg
    37141 tacaccgagc tcaatcagag gaatagtgat atcaagatcg tgtaaagaga caattctctt
    37201 tggatgagca ggaacagtga ctttgcgccc taaatcatca gtaaagacct gaacaggttc
    37261 tgtcgcgcgg gcaaagaatg acaacagtat cagcagagaa aaaaataaac gcatataact
    37321 cccattaaaa actgttcctg cactgccaga gcagtagcaa aaagaatggt ccaccaatca
    37381 atgaaataat gattccggca ggcagttgca aaggcaaaaa agccaaacga ccaatgctat
    37441 ccgccaggat gactaacaac gcccccgata cggcgctccc cagcagtaat gtcgtctgcc
    37501 cgccacgtag aataaaacgt gatatatggg gtgctatcaa tccgacaaaa ccgatgttac
    37561 caacacacga aacgcagacg gctgtcagga ttattggtgc ggcgacacgt aacaacgcca
    37621 gacgagaact attcacccct aacccggttg caacctgatg accaagcaga gcgatatccg
    37681 ccgtcctggc ggtgaacagc aataaaagag tggctggcaa catccaacag gctgaaatac
    37741 cgactaacat ccagttagct gcatgcaggc ttcccgataa ccacatcaat gcagtttgta
    37801 catctcgaac atctgcagtg gtcataaata caccgatacc ggcggcgaaa aaccaggaaa
    37861 caccaatacc aatcagaaca aatcgcgggc gggaaatatc gcgtgcacaa aaaatcacga
    37921 ttaaagccac caacaagcca ccagcaagac cagccactgg acgccagaac attcccagca
    37981 ttggaaactg gaaaatcagc cacagcaccg ccacgctaca tccttctttg acgccgatta
    38041 aaccaggatc ggcaaggccg ttacgcgcaa tggattgcat cgcggccccc gccataccta
    38101 acatggctcc gcatagcaat gccatgatga ctctcggcaa gcgaatatcc tgcacaatat
    38161 aacgtgcgtc tgtgcttacg ttttctggag caaacaacgc tcgtcctact tccgatgccg
    38221 ggacagaaaa agaaccatgc atcagaccga atatcagtat tccaaccgca aacagaaaca
    38281 gcccaccaag caacaccaga tttttagggc gtaccagtgt cgaaaactta ccaacacgta
    38341 gtgggcgtag cccgacgttc attatccgac tcatttgaac atcctcgtcg ccataatgac
    38401 aaaaacaggc gcaccaacta acgccgtcat gacaccggtc gctaactcat ggggcgtgaa
    38461 gagcgtccga gcaataatat ctgccagtaa taacaaaagc gcgccgacac aagctgaaag
    38521 agggagcaga acgcgcagat ccgctgatac cagacgccgg acaatctgcg gtactagcag
    38581 gccgacaaaa ccaatggggc cagcaatcga tacggctgcg ccacagagca acgcgatcgc
    38641 aagcagggtg aatgttcgcg tgcggagtac cgagacgccc agcccctgcg ccatgcgatc
    38701 acccagtgcc aacatgttaa gagagggcgc aaggtaaatg gctaacacaa atccccccag
    38761 agaaaaccag acggacgtac ccagcgtcac ccaatcctgc cccgctaaat cacctgctag
    38821 ccatgtccgc atcgccagca gtgtttgttc gtcaagaatc aggattgcag ccgttattga
    38881 ggacacgaac gccgatagcg ccacgccgca taaagtaact ttcattggcg ttaaccctga
    38941 acgccctgcg gaggaaagca gcaatatcaa caggaacaat aacgcgcccc ccgtagatgc
    39001 cagcagtggg cgtccagccg gaaaggccag tccaagcgca ctggcggcaa cgacagcaag
    39061 agcggctccg gcatttaagc ctaaaatatg tggctccccc aatgggttac gaatgacagc
    39121 ctgcaacaaa gcaccggcaa caccaagaga agcaccggta agcaatgctg ccgctaaacg
    39181 cggcaaacgg agtcttacaa ttacgttgtg atcgaagttt tgcggatcaa aatgaagaaa
    39241 tgcctgcaac accgtttgtg gtgcaataaa tcgggcacca atccctggat ataccacgca
    39301 tcccaccagc agtaaggtga ggaaaattac agtaaccagc cctgtgcgca tcatactttt
    39361 tctacaccac gaaacggcat gaagaacggc tttcccgtca gagtatttat tgatgcgtga
    39421 acatcaacgt caaagacagc ttttaccagc tcaggcgtgc aatgctcccc ctcattcaac
    39481 acacgcacca ctttcccctg gcgtaaaaat atcaacgtat caccataatt gacggcaaaa
    39541 ttcagatcat gcaacaccac gacgactgta cgcccgtgat gacgcgttaa atcatgtaat
    39601 aactcaagaa tttcaacctg ataacgtaaa tcaagccagg ttgtgggttc atccagtagg
    39661 atataaggcg tcttttgcgc caacaccatc gcaatccagc aacgctggcg ctgcccacca
    39721 gagagttttt caacaggtaa atgagcaaac tcctgcgtac cggttaattt cagggcctct
    39781 tctaccgcct gctcgtcggc gtcgctccat tgacgtatga aattttgcca gggaaaacgc
    39841 ccacgcgaca ccagttcata aacggttaag ccctccggca ataatggcga ctgcggcaag
    39901 atcccaagtc tacgggataa cgctttcgtt ggttgctcgt gaatcgcttt accatccagc
    39961 aatattgagc cgcccatggg ttgcaagata cgtgcaatgg tgctgagcaa cgtcgacttt
    40021 ccacagccgt ttgccccaac cagaacggtc attttttctg tgggaacgga gaaagaaaca
    40081 ccatcaacaa tgattttctt gtgatacccg gcagaaacat tgtcgaggat cagcccctgc
    40141 ccttttgcgt tatgtgccac gtgtacgcca acttaatcaa agacaaacaa aaataaaact
    40201 cattctcttt tgagagtttg acgccacaaa atgtagtagt caagaagtta aaagccat
//
LOCUS       DAZFFG010000028        39415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFFG010000028 DAZFFG010000000
VERSION     DAZFFG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.028"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            272..1927
                     /gene="aslA"
                     /locus_tag="ACHJ7J_001857"
     CDS             272..1927
                     /gene="aslA"
                     /locus_tag="ACHJ7J_001857"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO5000100.1"
                     /translation="MEFLFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(2095..3330)
                     /gene="aslB"
                     /locus_tag="ACHJ7J_001858"
     CDS             complement(2095..3330)
                     /gene="aslB"
                     /locus_tag="ACHJ7J_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5000101.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPEEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(3477..3553)
                     /locus_tag="ACHJ7J_001859"
     tRNA            complement(3477..3553)
                     /locus_tag="ACHJ7J_001859"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3517..3519),aa:Pro,seq:tgg)
     gene            complement(3596..3682)
                     /locus_tag="ACHJ7J_001860"
     tRNA            complement(3596..3682)
                     /locus_tag="ACHJ7J_001860"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3646..3648),aa:Leu,seq:cag)
     gene            complement(3703..3778)
                     /locus_tag="ACHJ7J_001861"
     tRNA            complement(3703..3778)
                     /locus_tag="ACHJ7J_001861"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3743..3745),aa:His,seq:gtg)
     gene            complement(3837..3913)
                     /locus_tag="ACHJ7J_001862"
     tRNA            complement(3837..3913)
                     /locus_tag="ACHJ7J_001862"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3877..3879),aa:Arg,seq:ccg)
     gene            complement(4016..5401)
                     /gene="yifK"
                     /locus_tag="ACHJ7J_001863"
     CDS             complement(4016..5401)
                     /gene="yifK"
                     /locus_tag="ACHJ7J_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5000102.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5592..6332)
                     /gene="wecG"
                     /locus_tag="ACHJ7J_001864"
     CDS             complement(5592..6332)
                     /gene="wecG"
                     /locus_tag="ACHJ7J_001864"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO5000103.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6335..7687)
                     /gene="wzyE"
                     /locus_tag="ACHJ7J_001865"
     CDS             complement(6335..7687)
                     /gene="wzyE"
                     /locus_tag="ACHJ7J_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO5000104.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7684..8763)
                     /gene="rffT"
                     /locus_tag="ACHJ7J_001866"
                     /gene_synonym="wecF"
     CDS             complement(7684..8763)
                     /gene="rffT"
                     /locus_tag="ACHJ7J_001866"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO5000105.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKSR
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALHAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8760..10010)
                     /gene="wzxE"
                     /locus_tag="ACHJ7J_001867"
     CDS             complement(8760..10010)
                     /gene="wzxE"
                     /locus_tag="ACHJ7J_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO5000106.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(10012..11142)
                     /gene="rffA"
                     /locus_tag="ACHJ7J_001868"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(10012..11142)
                     /gene="rffA"
                     /locus_tag="ACHJ7J_001868"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO5000107.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11147..11821)
                     /gene="rffC"
                     /locus_tag="ACHJ7J_001869"
                     /gene_synonym="wecD"
     CDS             complement(11147..11821)
                     /gene="rffC"
                     /locus_tag="ACHJ7J_001869"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO5000108.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11799..12680)
                     /gene="rfbA"
                     /locus_tag="ACHJ7J_001870"
     CDS             complement(11799..12680)
                     /gene="rfbA"
                     /locus_tag="ACHJ7J_001870"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5000109.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12699..13766)
                     /gene="rffG"
                     /locus_tag="ACHJ7J_001871"
     CDS             complement(12699..13766)
                     /gene="rffG"
                     /locus_tag="ACHJ7J_001871"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5000110.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13766..15028)
                     /gene="wecC"
                     /locus_tag="ACHJ7J_001872"
     CDS             complement(13766..15028)
                     /gene="wecC"
                     /locus_tag="ACHJ7J_001872"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO5000111.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(15025..16155)
                     /gene="wecB"
                     /locus_tag="ACHJ7J_001873"
     CDS             complement(15025..16155)
                     /gene="wecB"
                     /locus_tag="ACHJ7J_001873"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5000112.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16211..17257)
                     /gene="wzzE"
                     /locus_tag="ACHJ7J_001874"
     CDS             complement(16211..17257)
                     /gene="wzzE"
                     /locus_tag="ACHJ7J_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO5000113.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17269..18372)
                     /gene="wecA"
                     /locus_tag="ACHJ7J_001875"
     CDS             complement(17269..18372)
                     /gene="wecA"
                     /locus_tag="ACHJ7J_001875"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO5000114.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18612..19871)
                     /gene="rho"
                     /locus_tag="ACHJ7J_001876"
     CDS             complement(18612..19871)
                     /gene="rho"
                     /locus_tag="ACHJ7J_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO5000115.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19956..20057)
                     /gene="rhoL"
                     /locus_tag="ACHJ7J_001877"
     CDS             complement(19956..20057)
                     /gene="rhoL"
                     /locus_tag="ACHJ7J_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO5000116.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20198..20527)
                     /gene="trxA"
                     /locus_tag="ACHJ7J_001878"
     CDS             complement(20198..20527)
                     /gene="trxA"
                     /locus_tag="ACHJ7J_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO5000117.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20658..21923
                     /gene="rhlB"
                     /locus_tag="ACHJ7J_001879"
     CDS             20658..21923
                     /gene="rhlB"
                     /locus_tag="ACHJ7J_001879"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO5000118.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            22057..23541
                     /gene="gppA"
                     /locus_tag="ACHJ7J_001880"
     CDS             22057..23541
                     /gene="gppA"
                     /locus_tag="ACHJ7J_001880"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO5000119.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23588..25609)
                     /gene="rep"
                     /locus_tag="ACHJ7J_001881"
     CDS             complement(23588..25609)
                     /gene="rep"
                     /locus_tag="ACHJ7J_001881"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO5000120.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25826..26275
                     /locus_tag="ACHJ7J_001882"
     CDS             25826..26275
                     /locus_tag="ACHJ7J_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO5000121.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26474..26755
                     /gene="ppiC"
                     /locus_tag="ACHJ7J_001883"
     CDS             26474..26755
                     /gene="ppiC"
                     /locus_tag="ACHJ7J_001883"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO5000122.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26801..28276)
                     /gene="ilvC"
                     /locus_tag="ACHJ7J_001884"
     CDS             complement(26801..28276)
                     /gene="ilvC"
                     /locus_tag="ACHJ7J_001884"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO5000123.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28426..29319
                     /gene="ilvY"
                     /locus_tag="ACHJ7J_001885"
     CDS             28426..29319
                     /gene="ilvY"
                     /locus_tag="ACHJ7J_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO5000124.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPL
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(29371..30915)
                     /gene="ilvA"
                     /locus_tag="ACHJ7J_001886"
     CDS             complement(29371..30915)
                     /gene="ilvA"
                     /locus_tag="ACHJ7J_001886"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO5000125.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30918..32768)
                     /gene="ilvD"
                     /locus_tag="ACHJ7J_001887"
     CDS             complement(30918..32768)
                     /gene="ilvD"
                     /locus_tag="ACHJ7J_001887"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO5000126.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32833..33762)
                     /gene="ilvE"
                     /locus_tag="ACHJ7J_001888"
     CDS             complement(32833..33762)
                     /gene="ilvE"
                     /locus_tag="ACHJ7J_001888"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO5000127.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33782..34045)
                     /gene="ilvM"
                     /locus_tag="ACHJ7J_001889"
     CDS             complement(33782..34045)
                     /gene="ilvM"
                     /locus_tag="ACHJ7J_001889"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO5000128.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(34042..35688)
                     /gene="ilvG"
                     /locus_tag="ACHJ7J_001890"
     CDS             complement(34042..35688)
                     /gene="ilvG"
                     /locus_tag="ACHJ7J_001890"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO5000129.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35691..35741)
                     /gene="ilvX"
                     /locus_tag="ACHJ7J_001891"
     CDS             complement(35691..35741)
                     /gene="ilvX"
                     /locus_tag="ACHJ7J_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO5000130.1"
                     /translation="MNNSTKFCFSSFRTGN"
     gene            complement(35828..35926)
                     /gene="ilvL"
                     /locus_tag="ACHJ7J_001892"
     CDS             complement(35828..35926)
                     /gene="ilvL"
                     /locus_tag="ACHJ7J_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO5000131.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            36279..37799
                     /gene="yifB"
                     /locus_tag="ACHJ7J_001893"
     CDS             36279..37799
                     /gene="yifB"
                     /locus_tag="ACHJ7J_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO5000132.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(37824..38162)
                     /gene="maoP"
                     /locus_tag="ACHJ7J_001894"
     CDS             complement(37824..38162)
                     /gene="maoP"
                     /locus_tag="ACHJ7J_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO5000133.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            38281..39120
                     /gene="hdfR"
                     /locus_tag="ACHJ7J_001895"
     CDS             38281..39120
                     /gene="hdfR"
                     /locus_tag="ACHJ7J_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO5000134.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGDLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(39216..39291)
                     /locus_tag="ACHJ7J_001896"
     tRNA            complement(39216..39291)
                     /locus_tag="ACHJ7J_001896"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39256..39258),aa:Trp,seq:cca)
     gene            complement(39300..39376)
                     /locus_tag="ACHJ7J_001897"
     tRNA            complement(39300..39376)
                     /locus_tag="ACHJ7J_001897"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39340..39342),aa:Asp,seq:gtc)
BASE COUNT         9661 a        10734 c         9910 g         9110 t
ORIGIN      
        1 ttcattcaat taataacaca tatattaatt gcctttaaaa ctaaaaacag catcaataat
       61 caacgcgata taataaacct gccttacata tcaactgcgc cagaggtagg attgaaaacg
      121 ctctcctgat tttccaattc attttctgga taaataaata atttattttt gtcactatta
      181 tttatgtaat catcctgtca gggagaggga tctcaattat caatgcttaa ttacgtcatc
      241 attttgatga catgaaaccg gagcattttg catggaattt ttgttttcac ccaaacgtct
      301 tgttgttgct gtcgccgccg ctcttcctct catggccagc gcagcagata ccccgtcaac
      361 tgccaccgca cgcaaaggct ttgccggata cgatcaccca aaccagtatc tggttaaacc
      421 ggcgaccact attgccgaca atatgatgcc agtaatgcag catccggcgc aggataaaga
      481 aacccagcag aagctggcag aacttgagaa aaaaaccggt aagaaaccga atgtggttgt
      541 tttcttgctg gacgatgtgg gctggatgga cgtcggtttt aacggtggcg gcgtggcggt
      601 gggtaaccct acaccagata tcgacgccgt tgccagccag gggctgattt taacttcggc
      661 gtattctcaa ccaagctctt ccccaacccg cgccaccatt ctcaccggac aatactccat
      721 ccaccacggc attctgatgc cgccaatgta cgggcaaccg ggcgggctgc aagggttaac
      781 cacgctgccg cagttgctgc acgatcaggg ctacgtcact caggccatcg ggaaatggca
      841 tatgggggaa aacaaagagt cgcagccgca gaacgttggc tttgatgatt tccgtggctt
      901 taactcggtg tctgatatgt acaccgaatg gcgcgacgtt cacgtcaatc cggaagtggc
      961 cctgagtccg gaccgttctg aatacatcaa gcaattaccg ttcagcaaag atgacgttca
     1021 tgcggtgcgc ggcggcgaac aacaggccat tgccgacatt acgccgaaat atatggaaga
     1081 tctggatcaa cgctggatgg aatatggcgt taagttcctc gacaagatgg cgaagagtga
     1141 taagcctttc ttcctctatt acggcactcg cggctgtcac ttcgataact acccgaacgc
     1201 caaatatgcg ggtagctctc cggcacgcac ctcgtatggc gactgcatgg tggagatgaa
     1261 cgatgtgttc gctaatctgt ataaagcact ggagaaaaac ggtcagcttg ataacacgct
     1321 gattgtcttt acctccgata acggaccgga agccgaagta ccgccgcacg gacgcacacc
     1381 gttccgtggt gcgaaaggat ctacctggga aggcggcgtt cgcgttccga ctttcgtgta
     1441 ctggaaaggg atgatccaac cgcgtaaatc tgacggtatt gtcgatctgg cagatctctt
     1501 ccctaccgcg ctggatctgg cagggcatcc tggcgcgaaa gtggcgaatt tagtgccgaa
     1561 aaccaccttt atcgatggtg tggaccagac atctttcttc ctgggaacaa atggtcagtc
     1621 taaccgtaag gccgagcact acttcctcaa cggtaaactc gctgctgtgc gtatggatga
     1681 attcaagtat cacgtcctga ttcagcaacc ttacgcttat acccagagcg gatatcaggg
     1741 tggattcacc ggcacagtaa tgcaaacggc gggatcgtcg gtgtttaacc tgtacaccga
     1801 tccgcaggaa agcgactcca tcggcgtgcg ccatattccg atgggtgtac cgctacagac
     1861 cgaaatgcac gcgtatatgg agatcctgaa aaaatatcca ccacgcgcgc agattaaatc
     1921 tgattaagcc ggcgctatcg ctgagatctg cctttgccgg atgcgatgct gacgcatctt
     1981 atccagccta cagaacgctg caatttattg aatttgcacg atcatgtagg ccggataagg
     2041 cgtttacgcc gcatccggca atcaaccgca ggcggccaca aaatggccgc ttttttactt
     2101 actcaccacc agcaaatgcg catgcataat gtcgctggcc gggcgaccgt gcgccagcaa
     2161 atcagccata gctttaagat atggcggtag atggcggaaa taacgctgat acccggcaca
     2221 caaataattc agccccggtt tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc
     2281 tccccagcac gcttttaaca cgttacaact gcgacactgc gccggtaatt gcttaaattt
     2341 atcttcacca aacacctgct gttgcgggga atcgatcatt tctgcaattg tttgctggtg
     2401 catattcccc agtcgatatt gcggataaac atagtgatcg caggcgtaaa cgtcgccatt
     2461 gtgctcaaca atcaccgagc gcccacaggt tggctgatga tggcaaaccg cacccggcgc
     2521 accgacaaaa ttggcaaacg cccattcgat attcatcacg aaaatcttgc cgacgtcgcg
     2581 tttaatccag tggtcgaata tcgccaccag aaattcaccg aactcctcgg ggcgcacaga
     2641 ccattccgtc agttccccct gaatatcacc aggcgcatgt agtttcagtc cttcgcgggc
     2701 agccgtttca tcagccaggc gctcgaccac cggaataaac tggatgaatt ccactcccgc
     2761 atcgcacaaa aaatcatata cctgcaacgg ttgctgcgcg ctggtgcgat taacgcagac
     2821 cagcacgtta tagtcgacat gatgtttttg cagcagcgtc agggcacgca tcaccagttt
     2881 atgcgtgggt ctgccgcctt tggtcacgcg atattgattg tggatctcag ccggaccatc
     2941 cagcgataac ccaacaagaa aatgattttc tgccagaaat gcacaccatt catcatcgag
     3001 cagcacgccg ttagtctgga agctgttact tatcttcctg ccagcaccat atttcgcctg
     3061 tagcgctacg gcacggcggt aaaactccag cccgagtaac gttggttcgc cgccctgcca
     3121 ggtaaaagcc acttcgtttt gcggttcgct ggcagcgata tagtgacgga catacgcttc
     3181 cagcgtgtca tcgtccatat gcgtgactgg cttttcgcgg taaagggatt gtttttcgag
     3241 ataaaaacag tagtcacagt tcagattaca atcggaaccg ctcggtttcg ccatcacatg
     3301 aaaagcacgc gttggaacct gttgcagcat ggtcgctcct tgcctgaagt catccttcaa
     3361 tacgttaagg gcgaaatcat accgtggggc aatgagttat tacgggcgtt tattgcgaag
     3421 ggagggtgac gaaatgcaca gaaaacaaaa aaccccgccg aagcggggtt caaaattggt
     3481 cggcgagaga ggattcgaac ctccgaccca ctggtcccaa accagttgcg ctaccaagct
     3541 gcgctactcg ccgaaatact gctttttgaa tttttagttc aattctttaa agtcgtggtg
     3601 cgaggggggg gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt
     3661 ctaccaattc cgccaccttc gcattgtcac aacttctaat aatggggtgg ctaatgggat
     3721 tcgaacccac gacaactgga atcacaatcc agggctctac caactgagct atagccacca
     3781 ctacaaatct tgttacgcgg tattactacc accgcagctc aagcgccggg actaaatggc
     3841 gcgcccgaca ggattcgaac ctgagacctc tgcctccgga gggcagcgct ctatccagct
     3901 gagctacggg cgcttagcgc cgttgcgggg gtggataata cggacttccc acccctctgt
     3961 ctagtccctt tttaaataaa ttgcgcgttt ggttacggtt tgtgcgtttt gttgcttatt
     4021 cctccagttt atgcgctttc ccgtggcgat taaggccaaa aactttataa atcgccgtca
     4081 ccgccagcat aaagatgatg ccgacaaaca gcgacatacg cgtatcttca ttaaagtaca
     4141 tgccgatcaa aacgcaaatc aggaatgcca ttgttacgta gttggcccac gggaacagga
     4201 ttgagcggaa cggatggctg gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc
     4261 ttatcaatat cacaaaccat ggcaccatcc ccggaagcac gctggcactg tagacgtaga
     4321 caaacacacg ctgcggattg ggaatgatgt agttcaggca tgagccaatt agcagaatag
     4381 caatagatac tgccacaccc gcaaccggta cgccgtgacg ggaaactttc gccatcgccg
     4441 ccggtaactg acggtttttc gccagtgcgt agagcatacg tccgcaactg tacatgccgc
     4501 tgttacagcc agagagcgca gccgtcagca ccacaaagtt gataatgccc gccgctgcgg
     4561 taataccgat tttggcaaaa gtcagtacga acgggctgcc gttgctgcct atttcattcc
     4621 acgggaagat ggtgacgata acgaaaatcg cgcctacgta gaaaatcagg atccgccaca
     4681 gcaccttgcc tacggcactg cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa
     4741 tgccaatcag ctccacgccc tggtaggacg ccaccacaat acacagtgcg gtcaggaacc
     4801 ctttccagcc acccgcaaag aaaccgccat gctctgtgag attgctaaaa ccaatcgact
     4861 gccctccatt gccaaagcca aagaaaatca cgcccaggcc aatgacaatc atcacgataa
     4921 tcgtggtgac tttgatcatc gcgaaccaga actcgatttc gccgtacaac cgcaccgccg
     4981 ccagattcgc caacgccacc agcgccactg cgatcaatgc gggtatccac tgcgccatct
     5041 ctgggaacca gaactggaca taaacgccaa tggcggtgat ttcagagatc cccaccgcca
     5101 tccacataaa ccagtaagac caggcggtga gatagccaaa gaacgggctc atataacgat
     5161 gcgcataaac ggcgaacgaa ccggtaaccg gttcgaggaa caacatttcg cccattgaac
     5221 gcatgatgaa aaagacgaac agcccggcga tgatataggc caacaatacg gatggcccgg
     5281 cccatttcag ggtactggcg gcccccataa acaggccgac gccaatggtg ccccccaggg
     5341 cgatgagttc gatatgtcga gcttccagcc cacgctgtag ctctggtttg ttatctgcca
     5401 taaatcctcg tgttgtgttt gcatgctttc cggtgttacc ggttatcgtt atgggtacat
     5461 cgagtgttgc gaatgttttc gtaattcagg agaaatggca aataaagcat taaaaatttg
     5521 aatgctttgt gtaataaaaa agcagacagg cgacggagtg accactccgt cgctttacaa
     5581 agagaggaaa atcagaggtt gccggtgtag tgccagcgta aataacgcag caaacgaagc
     5641 tgacgcttaa tgcggctcgg ctgcgaaagc aggcggtaga gccactccag tcccagcgtt
     5701 tgccagattt tcggtgcgcg ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg
     5761 cccatataca gcgcatctgg atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt
     5821 ggcgatccca tcgcaacggt gacgatttgc gctccgctgg catgaatgcg ttcaaacagc
     5881 gcctgacgct gctcgggttt aaaataacca tcctgactgc caacgatatt cacattccat
     5941 tggttgcgca gtttagcttc agtttgcgcc agcacttcag gtttaccgcc cacaagaaat
     6001 accggcgtcc cttctttgcc tgcgcgcgcc atcaactctt cccagagatc ggcaccggca
     6061 acgcgggaaa cctgcgcctg cgggtacttt ttacgtactg aacgtacaac gctgatgcca
     6121 tccgcatatt taaattcggc agcgttaatt aactccctga cctcggcgtt atcttcaatg
     6181 gtcagcattt tttcagcatt aatggcaacc agcgttccct gcttaagctg cccgtcagca
     6241 aacagataat cgagggcgtg ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc
     6301 gtataggtcg gtgccgtggt gttgttattc attgttatcc ttcaacctgc gtccggagcg
     6361 atgattttgt acgtttatga atgagtccgg cgctttcaaa aagccagtac aacagttttg
     6421 cgatcatcag acatgcgccg aagaccacga taaaaaagac tacgcgtgag acaaacgaat
     6481 ccagcccttc acgtgccagc acgatcatat tgaagatcgc cccaaagcag aaactgtgca
     6541 atatcgcagc cttatagcga ttagtctcgc ggttgcccag ctcatacagc cagtcgaacc
     6601 atttgatgat cagtccaacc acaatcgccc cgagcgggat gaacaacgcg ccgcccatca
     6661 ccaccagtga gcctataagc gtaggcgaga tcgccagtcc ggagtggtta ttcagcactt
     6721 cccaggtaaa gtagttggct gagttcagca ccatactcgg gcgacccggc cacagccagg
     6781 aagggataaa gacatagaaa tcgcggacaa ttggagccag gccctggaag tcgatgttgt
     6841 cgtagttctg caacagcaac gccagattct cccacggcga gaaggtgtcg cgagtgagat
     6901 agagaaacgt atagaacgct tcatcgccgc tcacattcat tccatagcgt tttagcgcca
     6961 gccagaacat gccaacaatc cccagcacgc ccgccgccgc cagcatccac aacgaaatcc
     7021 agccgcgaat tatgccaata aacaggaaga tagcgaatgc gatgatgata ttggcgcgag
     7081 tgccgccgac aatcatataa gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca
     7141 gccacgcttt gctgtcctgg cgcagaaagt agaccaccag catcgccggg atgaaaaagt
     7201 aaaagaagcg ttttaacgcc acgccggaga cttcactgga aaagatctga ctgtaggagt
     7261 taagccggaa cagcaaaaag ccgttgtgca tgaagaagat gccgacgctt accagcgcga
     7321 tacccatcag gatcacccac gtaagattgg tctccacgcg gttcatggta aacagcggac
     7381 ggcgcggtac atcagcaacg cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg
     7441 cgtagaagca gcccgcagaa agcaacgcct gcaacaagat ttctggcggc gcgacaccaa
     7501 catcaaagcg aaataccagc acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa
     7561 acagcaatga aaagaagaca ttgaagttaa agcgcacacg gcgaaactca aaccaggtca
     7621 gcgtagcaat aaacagcgtg cagagcagcc agaccacaaa caggccactg aattgcagca
     7681 gactcatgcg acctccccgg cggcaatcgc caacgcccgc tgccagcctt gtagatagtt
     7741 agggctaaag aaggcaatgg tgtttttatc taccgacgcc aactggcgct gcgcctcacg
     7801 cacaatatcc tcattgagat cgtcggtagt aaacagcact ggcaaatgct gttccgtcat
     7861 atcctgccag aacggatttt cccggttaag cacacaagga atgcccgcct gaatcagtaa
     7921 gcaaagcgta ccaatgccct gctggcgggc aaaaataaag taaccaagat cgcactgacg
     7981 aagtagcgtg agataggcgt caaactccag tttttcactc agaacttgca gattttcttc
     8041 gctgaataac tccagccccg cctgacgaac ttcctcgatg tacgcttcgt tattaggcgg
     8101 atatcccatc ggcaccacca cttttaccgt atcgccaaat tgctgatgaa cggcgtgcaa
     8161 ggcagcaata tgctcattgc tgcggtcgcc ggagttcccc accagaatgg tcattttccc
     8221 ttcacgttgc cgatcgttcg ccatcgtatt gagagaagga tccatccgcg tcgggaagta
     8281 cagcagttcg ccccgcacct ttggatgcgt tttggcaaaa aagctcaaat caccgcgggt
     8341 ggcaaataca cagccgactc gcttttgcgc caggcgacgt agcgggtaaa aaagcttata
     8401 tctcaagcca ctggaaagct catacagatc tgccccccag atatgccaga aaaactggcg
     8461 ggacttaatg ccaccactca gcaaagccag ccacagtttg ggattgaact gaccgtggaa
     8521 gaaaaaacgc tgctgacggt tagcttttgc tttcgcgatg accgcttccg ccagcgattt
     8581 tttcccaggg aaaaattgca cagaaagcgc cggacagcta tcacttaagc cgtcgtcctt
     8641 gccaacaacc ataaactcgc gcgcgtgctc gctcgtcgcg gccagcgcgt cattgaaaaa
     8701 ccgcaaaacg gttcggttat ggtgagggat atccgatccc agtacgtgaa tcagtacagt
     8761 catgcccgcc tacgccagag taaaaacacg ccacaacaaa gagaaaaata gacgatatag
     8821 gttgccatat aggcctgcgc cgcgcccagt gcaccatgcg cagggattag ccagtgggca
     8881 aataccatca ataaagtgaa ctggctgact tccgccagaa tataaaaccg cagtgaagct
     8941 ttggcgatca ccagataacc aaagacataa gcgcccactt ttaacacatc acccactaac
     9001 tgccaggcaa agagatcgcg catagcggta aatttattcg acaacagcag ccagatagca
     9061 aaatcacgca gcaaccagac ggtaaaactc gccgccgcca ctgccggtaa gacgaatttc
     9121 agcgatttaa ccacttcccg ggtgatatcg cgcttttccg ttagccgcga caacgtgggc
     9181 agcaaatata cgctgaacga tgccgtaata aattgcaggt aggcatcgga aatactgctc
     9241 accccttgcc agatccccac ctcatcccag ctatactgcg ccgccagcag tttacgcatc
     9301 atgatgtaag caacaggcaa ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc
     9361 aactgccctg ccagaccgtt atcccagctg ggtttcagat agcttaacgg gatgacacca
     9421 cgtttgatca acatgatggc ggcaggaatc accaccagcg cgggaatcag cgccagaccc
     9481 agcaacgccc cttcataacc gcccaaacgg taactgacgt agtacgcaag aacgccaatc
     9541 aagctgccaa caatcagaga taacgcatta cctgcggcat cgcgaaagcc tttcatcaac
     9601 gccagtaaca ggttgcccca ggcgatcccc atttgcacca acgccactaa acgtaccagc
     9661 ccctgatagt cggtattacc aaacaatccc tggctgattg gcgctgctgc cagcacaaaa
     9721 accagcgcca tcagcgtaga gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg
     9781 cgcagctgtt gcggattatc atggtactgg gcaacgtatt tggttacacc gttaaagatg
     9841 ccagccccgg caagcacgcc gagcacggta atcaactggc ggaaatttgc cgccagccca
     9901 agccccgccg gaccaaatga caccgccagc aacttaccga ccagtaaccc ggccccaatc
     9961 ttgaccagtg tactggccgc cgtccacaag gacgctttcg ccaacgacat atcaggagaa
    10021 gtagttcaac aaagtcgcaa ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag
    10081 cggcaggcgc agcaggcgct cgctctcttt ggtggtgtag cgatcttcac cgtggaactc
    10141 accaaagcgt tcccccgcag ggcaaccgtg cagcggaatg taatggaaca ccgccatgat
    10201 ttccgcttct ttcagaaagt taatcaacgc gctccggtca tcaatatccc gcagtttaat
    10261 gtagaacata tgcgcgttct gcacgcagcc atcgggaatc gacggcagct cgatacgccc
    10321 tgctttcgcc agaggtgcta acgcatcgta gtagttttgc cacagcgcca gacgttgctg
    10381 gttgatacga tccgctgctt ccagttgcgc ccacaggtac gcagcttgca gatcggacat
    10441 caaatagctg gagccaatat cgcgccaggt atatttatcg acctgaccac ggaagaactg
    10501 gctgcggttt gtgccttttt cacggatgat ctcggctcgt tcgatcaacg ctttatcgtt
    10561 aatcagtgtc gcgccgcctt caccgcccgc cgtgtagttt ttggtttcat ggaagctaaa
    10621 gcagccaata tgaccaatgg ttcccagtgc acgccctttg taagtggaca tcacgccctg
    10681 agcagcatct tccaccacaa acagattatg ctttttcgcc aacgccataa tggtgtccat
    10741 ttcgcaggcc acgcccgcgt aatgcaccgg cacgataacg cgcgttttgt cggtgatcgc
    10801 cgcttcaatc aacgtttcgt cgatgttcat ggtgtccggg cgaacatcca caaaaacgat
    10861 ttttgcgcca cgcagcacaa aggcattggc ggtggagaca aaggtgtagc tcggcatgat
    10921 cacttcatcg ccaggctgga tatcgagcag cagcgccgcc atctccagcg aagcggtgca
    10981 ggacggcgtc agtaacactt tggcgctgcc aaaacgttgc tccagccact gctggcagcg
    11041 acgggtaaaa ccgccatcgc cacacagttt gccgctaccc attgccgact gcatatagtc
    11101 gagttcggtt cccaccaccg gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc
    11161 agtacgcggt gctttctaca ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt
    11221 tgcccatttg ggtcgccacc cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg
    11281 ccgtttgcat cagctcagca cctgcaccgc gtccagccag caggccaatt cgcgcatctg
    11341 tcgcattgag ttcccgtaaa gagacataac cgcgaatatc gccggacgcc gcacgtaaaa
    11401 tcagacattg atgatcaaag gtgccgcgca cggcattttc aatccactgt gcataaaagc
    11461 gactgctggc gtcaggcgca taccacggcg cacgaaaacg gctttgcgca aatgcggcgc
    11521 tggctaactg acgtaatgcg ggaatatcgg tctcttgtgc cactacagca ccgctatcac
    11581 tggcattgtt cacgggtagc gccaaatcaa cttcaccttc taccagggag aatcccagct
    11641 gttgcagggc atccagttcc cccgtatttg atgccgcaat tttggcctgc acccgtgacc
    11701 acggcgctaa cgcgtctggc gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc
    11761 tgttaacacc aaagaaggcg ttttcccagg ttagtggctc aatactggcg cggacgggca
    11821 cgaagtaact ccagcagata ttggccgtag ccagttttcg ctaatgaact ggcagcacgc
    11881 ttcacaccct cgtcatcgag ccagccgtta cgccaggcga tctcttccag gcaggcaatc
    11941 ttaaagccct ggcgtttttc caccgtctgt acaaaggtgc tggcttcaat caggctgtcg
    12001 tgagtgccag tgtccagcca ggcaaatccg cgcccgagca gttcaacggt caggttgccc
    12061 gcctcgaggt acatctggtt gatggaggta atctccagtt caccacgctc cgacggcttc
    12121 acctgctttg cgtactccac gactttactg tcgtagaaat aaagcccggt caccgcccag
    12181 tttgacttcg gctgttttgg cttttcttcc agcgagatag cgcggaaatt gtcgtcaaac
    12241 tccaccacgc caaagcgttc cgggtccatc acctgatagc caaaaaccgt cgccccttcc
    12301 gtgcgcgccg caacatgacg cagcttcgga ctgaagccct gaccgaagaa gatgttatcg
    12361 cccagcacca gacaagaagg ttcaccatta aggaaggttt caccgatgat aaaggcctgc
    12421 gccagaccgt ccgggctggg ctgttcggca tattccagct ggataccgaa ctcactacca
    12481 tcgcccagca ggcgctggaa ataaccttta tcttccggcg tagtgatgat gagaatttcg
    12541 cggataccgg ccagcatcag caccgacagc ggatagtaaa tcattggctt atcgtaaatc
    12601 ggcaacagtt gcttcgatac gccgcgcgta atcggatgca atcgggtacc ggaaccgccc
    12661 gccaggataa tacctttcat gtttgcctcc gctgaaaatt agcctttcag acctaaacgc
    12721 tcgccctgat agctgccgtc ctgcacctgc ttccaccagc tttcattagc cagataccac
    12781 tgaaccgttt tacgcattcc actttcaaag gtttcctgcg gcagccagcc aagttcacgg
    12841 gcaattttcg aagcatcaat ggcataacgc agatcatgcc ccggacggtc agcgacaaag
    12901 gtgatcaggt cacgatagtg cgccacgccg tgcggcttgt tcggagccag ttcttccagc
    12961 agctcgcaaa tggtttccac aacatcgaga ttcttacgct cgttgtgacc accaatatta
    13021 taggtttcac cgactttccc ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc
    13081 acatacagcc agtcgcggat ttgctgcccg ttgccatata ccggcagcga tttacccgcc
    13141 agcgcgttga ggatcatcag cgggatcagt ttttccggaa agtggtaagg gccgtagtta
    13201 ttcgagcagt tggtgataag cgtcggcaga ccataggtcc gcagccaggc gcgcaccaga
    13261 tggtcgctgc tggcttttga cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg
    13321 gtgaagaaat catccgtcga gtgcaggtca ccatatactt cgtcagtgga gatatgatga
    13381 aaacggaacg ctgatttttt atcttccgtt agcgcattcc agtaagcccg cgccgcttca
    13441 agcaatgtat aagtcccgac aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa
    13501 cggtcaacat ggctttcggc tgccagatgc atgacacagt ctggctgatg ctcagtgaat
    13561 acgcgcgcca gttctgcccg atcgcagata tcaactttct caaaggcaaa gcgctcgctt
    13621 tgcgcgaccg gtgccagcga catcaggttt ccggcgtagg tcagcttatc gaccactacc
    13681 accgcatcgc tcgtttcgtt gatgatataa cgcaccagcg ccgagccaat aaacccggca
    13741 ccgcctgtta tcagaatctt tctcatcagc gccagactcc tttggcatcg acgacatact
    13801 gctgatggac attgtcgcca ttgataactt tgaactgact atgatcgacc agcatcacca
    13861 gcacgtctgc cgttgccagc gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt
    13921 ttttcggcaa ctggtggatg ttaggctcaa caaccagagt ttcgccgcta tgccactggg
    13981 cgatcagttc agcgatttcc atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa
    14041 acgccagacc aaagcaggcg attttcagtt cactggcgcg tttatcggta gccgccaggc
    14101 aatcagccac cgccgctttc acctgatcga taacccagaa cggtttgtga tcgttcactt
    14161 cgcgcgcggt acggataagc cgcgcctgct gggggttctg tgccacgata aaccacggat
    14221 caacagcaat gcagtggccg cccacgccag ggccaggctg aagaatatta acgcgagggt
    14281 gacgattcgc caggcgaatc agttcccaga cgttaatccc ctgatcggca caaatcagcg
    14341 acaattcatt agcaaaagcg atattcacat cgcggaagct gttttcggta agcttacaca
    14401 tttccgccgt ccgcgagtta gtgacgacac actcaccttc gaggaaaatt ttgtacagtt
    14461 cgctggcgcg ggccgaacaa accggcgtca taccaccaat cacgcgatcg tttttaatca
    14521 gctcgaccat aacctggccc ggtaacacgc gttccgggca gtaagcaatg ttgacgtccg
    14581 cctgctcgcc cacctgctgt gggaaagtga gatccggacg catctctgct aaccattctg
    14641 ccatcttctc ggttgacccc accggcgagg tggattcaag gatcaccagc gcgccttttt
    14701 tcagcactgg cgcaatggag cgagcagccg attcaacgta ggtcatatct ggctcatgat
    14761 cgcctttaaa cggcgtgggt acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc
    14821 tcgctcgtaa aaaaccgcct tctacggcag tttttactac gctcgccaaa tcaggctcga
    14881 cgatatggat ttcgccacga ttgatggtat caaccgcgtg ttggttgata tcgacaccaa
    14941 tcacctgttt ttgccgtgag gcaaacgctg ctgctgtagg cagcccgatg taacccagtc
    15001 cgataacaga aatggtcgca aaactcatag tgatatccga ttatttttta acgcttccag
    15061 aatgcgagag catgcctgac catcaccata cgggttatgg gcgcggctca tagtttgata
    15121 ttcgttttcg tcttttaaaa gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc
    15181 taccagacgc accgtacccg ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac
    15241 cagcaccggt ttccccagcg aaggcgcttc ttcctgaatg ccgcctgagt cggtcaaaat
    15301 cagccaggcg tggttcatca gccagacaaa cggtaaatac tcctggggat cgatcagaat
    15361 gacatttttc acatgcccca gaatgcgatt gaccggctct ctgacgttcg ggttgagatg
    15421 caccggatag acaatctgga tgtcctggtg cgtggtggcg atgtctgcca gcgcgtggca
    15481 gatttcttca aagccacgac cgaaactctc acgcctgtga ccggtcacca gaatcatctt
    15541 tttatcgggg tcgataaacg ggtaatttgc cgcaagttct gaacgcagcg tgtcgctgct
    15601 catcacctgg tcacgcaccc ataacagtgc atcaatgact gtattaccgg taatgaagat
    15661 tcggctatct gcaacgtttt cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa
    15721 atgatacatc gccagatgcc cggtcaatgt acggttagcc tcttccggcc acggcgaata
    15781 gagatcgccc gtgcgtaaac cagcctcaac gtgaccaaca ggaatacgct gataaaacgc
    15841 cgccaggctg gttgccagcg tcgtcgtcgt atcgccgtga accagcacga cgtctggttt
    15901 gaactcggca agaataggtt ttagcccttc cagaatccga caggttatct ctgtcaggcc
    15961 ctgtcctggc tgcattatgt tgagatcgta gtcaggtaca atggaaaaga gtttcagcac
    16021 ctgatcgagc atctcccgat gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc
    16081 ttttgccaac gcatgcacca acggagccat cttgatggct tccgggcgcg taccaaatac
    16141 agtcagtact ttcacatcga ttctcttcga ataagcggcg agcgcctttg cgctcaccgc
    16201 agcagtgttg ctatttcgag caacggcggg ttaatgcgac accagccccg atcagccccc
    16261 cgacaatgcc ccacataatc atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt
    16321 cttccggcgt acgcaaatag cgataggtct gaaaacgcgg atccagggtt ggaccaacat
    16381 tcagggtgtt taacatggcc cgattctgat catagtcgag atcaaaggcc ggaccgacgg
    16441 cctgtaaatt ttccagtcga gcctggagca ttggacgccc aagcaggaac atttctgaat
    16501 caggtaattc ctcggcaggc acatctgtcg cactgcgcga aatattatgc tgctcagcaa
    16561 ttttcagcgc ctgctcaatg ctgttcatcc ggcggtcgta gatggctttc gccacctctt
    16621 cctgacgctt cacctgagct ttcatctgga tggtgcgtgc cgcccatgcg cctttcagct
    16681 catcattcag atggctggct gcacgctggc tggcaaaagc aacatactga cgtaacaggt
    16741 tattagcgtc aggcgcggtt tcggcaataa gcttcacgct gtcattgacc gcgcgggtaa
    16801 agtctccggg gataaactgg atgttgttaa tcatttcatc cagcaacgcc gcatcggctt
    16861 tgctgttgcc caccatccgc tgcttgtaat agtcggtttg cagccagaac tctctgcggg
    16921 tatcccacga ggccagttgc ataacaaact ctttataagc ttcgtccatg accgatggtt
    16981 ggtcggcaga agccatgttt gaacggacat ccaggttacg caaaaattgc tgctgcgagt
    17041 aatatccccc cagcatattc accgttggac gatcggtaat cgccgtcgag ctccactcct
    17101 gacgagcaaa aaacgtatac gccagcgcga ttaacgcaaa cgccagcccc atgccaataa
    17161 tccatagctt cccagcccac aaggtacgaa acaacccacg aatatccagt tcattttcag
    17221 cgtcttcggc cggtttccca ggcattggtt gtgtcatcac atcctcattt atttggttaa
    17281 attggggctg ccaccacgat ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc
    17341 aactttccag gcacgcttaa tgcaatatcc atagaggaag aatgctagca aaaagagcac
    17401 cagcatgacc cactccggga caaaatgaga atattctgcc agcacgccaa tggaagcgag
    17461 cagtgctgcg gcaagggtaa tcagcacaaa cgcctggcgg gaagtaaacc cggcacgcat
    17521 gatcaaatgg tgaatatgct gacggtcagg agagaatggg ctcatgcctt tacgcaggcg
    17581 acggtacata atcgccacca tatccattag cggaatggcg attatccaca aagcggtaac
    17641 cgggctgatg ggatgggttt tgccctgggt cgtttcgagc aggatccaga tcacggtaaa
    17701 accaatcagc gtactgcccg catcacccat aaagactttg tagcggcgac ccaggatacc
    17761 aaggttaagc atgatgtatg gcaggatggc ggcgatcatc gcaaagcacc agattgcgag
    17821 gctggtttgc ccgtcgaacc acaaaatcat accgattgct gcaaacgaga cgcaggacaa
    17881 cccgcccagc aagccatcaa tgccatcaac catgttgaac gcattaatgg ccgcccagac
    17941 ggcaaatagc gtcaggaagt aaccaaacgg tccgagcacc atctcccagg agccaaagat
    18001 ataacccagg ctactgagat aaagcttgcc gaacaccatc ataacaatgc caacagcggc
    18061 ctgtatggtg gcacggattt ttacgctgat atcaaaacgg tcatccagcg cgccaatgaa
    18121 aacaagcaca ccggcacaag cgagatagag agatgcatgc ggaatatagt aatcgacaat
    18181 tccgaacgtg aagcaaatcc ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc
    18241 ctggtgacgt ttgcggaagt ttggtttatc cactaaaccg actttttttg ccaccttacg
    18301 ggcaaaaaac agaaacagtg tcgtgaataa aaaaatactg atgagatcag tactcactgt
    18361 cagtaaattc acaatgcatg ctctcagaga aaattattag cagaagtata accacgaaga
    18421 cctttattca gaagggaaag tccgttccga acccaccagt cctgtgcaaa aattaaccat
    18481 ttgatatgca aattgcataa catgccagca aattccagga attttcctaa catcgccagc
    18541 gcggcattaa gattacagat ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc
    18601 ataagacaaa tttatgagcg tttcatcatt tcgaagaaat cgtcattggt cttggtcatt
    18661 gccagtttat taatgaggaa ttccattgca tcgatttcgc ccatcgggtg aatgattttg
    18721 cgcaggatcc acattttctg cagttcttcc tgagtcgtga gcagctcttc tttacgggta
    18781 ccggaacggt tgtagtcgat agccgggaag acgcgttttt cagcgatctt acgagagagg
    18841 tgcagttcca tgttgcctgt acctttaaac tcttcgtaga taacttcgtc cattttagaa
    18901 ccggtatcga taagcgccgt cgcgataatg gtcaggctgc cgccctcttc cacgttacgc
    18961 gccgcaccaa agaagcgttt cggacgatga agggcattgg cgtccacacc accggtcaac
    19021 actttacctg acgccggaac aacggtgttg taagcgcgcg ccagacgagt gatggagtcg
    19081 agcagaatga taacgtcttt cttgtgctca accaggcgtt tcgccttctc gatcaccatt
    19141 tccgcaacct gaacgtggcg agatgcgggt tcgtcaaagg tagaagcaac aacttcacct
    19201 tttaccagtc gctgcatctc ggttacttct tccggacgtt cgtcgatcag cagaaccatc
    19261 agcacacaat ccgggtggtt gtaagcaatg ctctgagcaa tgttctgcag cagcatggtt
    19321 ttaccggctt tcggcggtgc cacaatcaga ccacgctgac cacgaccgat aggtgatgcc
    19381 agatccagta cgcgagcagt taaatcttcc gtagaaccgt taccacgttc catacgcaga
    19441 cgagagtttg cgtgcagcgg ggttaagttc tcaaagagga ttttgttgcg ggcgttttca
    19501 ggtttgtcga agttaacttc gttaactttc agcagcgcaa aatagcgttc accttctttc
    19561 ggcgggcgaa tcttaccaga gatggtatca ccagtgcgga ggttgaaacg gcggatttgg
    19621 ctaggggaaa cgtagatgtc atcaggaccg gcgaggtagg agctgtctgc ggaacggagg
    19681 aaaccaaatc catcctgcaa tatctccagt acgccatcac caaagatatc ttcgccactc
    19741 tttgcgtgct gcttcaggat ggcaaaaata atgtcctgct tacgcatacg agccaggttt
    19801 tccagcccca tattttcgcc gagagtgatc agctcagaaa ccggcgtatt ctttaattcg
    19861 gtaagattca tagtggtgtg agttcttaaa cttggggtaa atctcgaact taatgttgtg
    19921 aatggtatgg caggatcatc catgcctgtt tacggtcatc gactcatgtc ttttcgctgc
    19981 ctggtcacag gagagtacgc agaactgaaa cgacaagacg gattgagtga cgagccagaa
    20041 atctgttcac ttcgcattta agataaaacg ggaagcgttg gaaattacaa gattcaaact
    20101 taataaggta tgtttaatac gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc
    20161 agtttttagc gacggggcac ccgaacatga aattccctta cgccaggtta gcgtcgagga
    20221 actctttcaa ctgaccttta gacagtgcgc ccactttggt tgccgccact tcaccgtttt
    20281 tgaacagcag cagagtcggg ataccacgga tgccatattt cggcgcagtg ccagggtttt
    20341 gatcgatgtt cagttttgca acggtcagtt tgccctgata ttcgtcagcg atttcatcca
    20401 gaatcggggc gatcattttg cacggaccgc accactctgc ccagaaatcg acgaggatcg
    20461 ccccgtccgc tttgagtaca tccgtgtcaa aactgtcgtc agtcaggtga ataattttat
    20521 cgctcatata taactccaca ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca
    20581 ttaaccaact aaaggttgac tttatttcac cggatacgct ttcgtaaagc aatagtaagc
    20641 tgatattcta ccacactatg agcaaaacac atttaacaga acagaagttt tccgacttcg
    20701 ccctgcatcc gaaggttgta gaagcccttg aaaaaaaagg gtttcataac tgtacgccca
    20761 ttcaggcact ggcccttccg ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa
    20821 ccggtaccgg gaaaacgatg gcgtttctta cgtcaacgtt tcattatctt ctctctcatc
    20881 ctgcgattgc cgatcgcaag gtgaatcagc cgcgtgcctt aattatggca ccgacgcgtg
    20941 aacttgccgt gcagatccat gccgatgcgg aaccgctggc ggaagctacc ggcctgaagc
    21001 tgggtctggc ttacggtggt gatggttacg acaaacagct gaaagtgctg gaaagcggcg
    21061 ttgacattct gattggcacc acgggtcgtt taattgacta cgccaagcag aaccacatta
    21121 acctcggtgc cattcaggta gtggtactgg acgaagccga tcgcatgtac gatctgggct
    21181 ttattaaaga tatccgctgg ctgttccgcc gtatgccgcc tgcaaaccag cgcctcaaca
    21241 tgctgttctc cgccacgctt tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca
    21301 atgccgaata tattgaagtg gaaccggaac agaaaacggg ccaccgtatt aaagaagagc
    21361 ttttctaccc ttctaacgaa gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt
    21421 ggccagacag agcgattatt ttcgccaaca ccaaacaccg ttgtgaagag atctggggcc
    21481 acctggcggc agatggtcat cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac
    21541 gtctgcgtat tcttgatgaa tttacccgtg gcgatctgga tattctggtt gccaccgacg
    21601 ttgccgcgcg tggtttgcat attccggcag tgacgcacgt ctttaactac gatttacccg
    21661 atgactgtga agattacgtt caccgtattg gtcgtacagg tcgcgcaggc gcaagcggtc
    21721 actctattag cctggcgtgt gaagagtatg cattgaattt gcctgctatt gagacctata
    21781 ttggtcactc aattccggta agcaaataca atccggacgc attgatgacc gatctgccaa
    21841 aaccgctgcg cctcacgcgc ccgcgtacag gcaatggtcc gcgtcgtact ggcgctccgc
    21901 gtaatcgtcg tcgttcaggt taaataaaat gtcatactca aaatcattca aactgtatca
    21961 agatggcaag agaatgaatc ctgaatcact tactgtagta agtgattcag gtgaaagaat
    22021 gcagccaaca cagagacaga ttgaaggatg aagagtatgg gttccacctc gtcgctgtat
    22081 gcagccattg atctcggttc gaatagtttt catatgctgg ttgtgcgcga ggtggctgga
    22141 agcatccaga cgctgacgcg aataaaacgc aaagtgcgtc tggctgctgg cctgaatagt
    22201 gaaaatgccc tctctaatga agcaatggag cgcggttggc aatgtctgcg cctgtttgct
    22261 gaacgtctgc aagatattcc tccctcgcaa attcgcgttg tcgctacggc gacgttacgc
    22321 cttgccgtca atgcgggtga ttttattgcc aaagcgcagg aaattctcgg ttgtccggta
    22381 caggtgatca gcggtgaaga ggaagcacgt ctgatttatc agggcgttgc tcacaccact
    22441 ggtggtgccg atcagcgcct ggtggtggat atcggcggag ccagtactga actggtaacc
    22501 ggcacgggtg cacaaactac ctcgttgttc agcctgtcga tgggctgcgt cacctggctg
    22561 gaacgctatt ttgccgatcg taatctgggg caggaaaatt ttgatgctgc agaaaaagcg
    22621 gcacgcgaag tgttacgtcc ggttgccgat gaattacggt atcacggctg gaaagtgtgc
    22681 gttggtgctt ccggcaccgt gcaggcgtta caggaaatca tgatggcaca ggggatggat
    22741 gaacgcatta ccctggaaaa gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg
    22801 ttggaggaac tggagattga cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt
    22861 ctggcgatcc tgatcgccat ttttaccgaa ctgaatattc agtgtatgac cctggcgggc
    22921 ggtgcgctgc gtgaaggcct ggtctacggc atgttgcatc tggccgtcga gcaggatatt
    22981 cgcagccgta cgctgcgtaa tattcaacgc cgctttatga tcgatattga tcaggcacag
    23041 cgcgtagcca aagttgcggc taacttcttc gatcaggtgg aaaacgaatg gcatcttgaa
    23101 gcaataagcc gcgatttgct catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt
    23161 gacttcaaac aagcgccgca acacgctgct tatctggtgc gtaatctgga tcttcccggt
    23221 tttacccccg cacagaaaaa attgctggcg acgctactgc tcaaccagac taatccggtc
    23281 gatctctcat cgctgcatca gcaaaatgcc gtaccgccgc gcgtcgcaga acaactctgc
    23341 cgtttactcc gcctggctat catttttgcc agccgtcgcc gtgacgatct cgtgccagag
    23401 atgacattac aggctaacca tgaactgttg accttgacgc ttccgcaagg ttggctaacc
    23461 caacatccgc tgggtaaaga gattattgct caggaaagcc agtggcagag ctatgtccac
    23521 tggccgctgg aagtgcatta atgagtaagt gccggatgcg atgctgacgc atcttttccg
    23581 gccttgatta ttttcctcgt tttgccgcca tcatcgcttt cagattcgcc agatgacttt
    23641 gccctttctg catccgttct tcggcgctga ccactttgcg ctcctgttcc caaatcagat
    23701 catcctgcgg cagctccagc aaaaagcggc tcggctccgg gcgcaccagt tcgccgtact
    23761 gacggcgttc tttacacagc gtaaaggtca attccttctg ggcgcgggta atgccgacat
    23821 aggccagccg ccgctcctca tcgatattat cttcatcgat actgctctgg tgcggcaaaa
    23881 acccttcttc cataccgacc atgtagacat aaggaaactc cagtcctttt gacgcgtgga
    23941 gagtcatcag ttgcacctga tccagctctt cttcactctc accacgctcc atcatgtcgc
    24001 gcaaagtaaa gcgcgtcacc acctgggtga gcgtcatcgg ctcatccagt tcgctgcctt
    24061 ccagcatctc cgtcatccag ctaaacagtt ggttgacgtt cttcatgcgc atttcggcgg
    24121 ctttcgggct gggcgatgtt tcgtacagcc aggattcata atccatgcca tggatcagat
    24181 cacgcaccgc ggcaatcggc tcccgctccg ccagacgctg gatttctgcc aaccagtgag
    24241 tgaagcgggt caatgcttca taaccacgtc cgctaagcgt ctgactcagg cccatatcaa
    24301 agctggcggt aaacatgctt ttattgcgcg tcatcgccca ttcacccaac tttttcagcg
    24361 tcgccgggcc aatctctcgc ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt
    24421 ccgggttagt cagcacgcgc agataagcca gcaagtcctt gatttcagga cgagagaaaa
    24481 acgacgtacc accagaaatt ttgtacggga tgcggttttg catcaggaat ttttcaaaca
    24541 cccgcgactg atggttaccg cgataaagaa tggcgtaatc tttgtactgc gttttattga
    24601 cgaagtgatg ggcgatcagc tcgccagtaa cgcgctcagc ttcatgttcc tcgttattcg
    24661 cgcttaaaac ttttagctct gtgccgtaac ccagttcgga gaacagacgc ttttcaaaga
    24721 cgtgcgggtt attggcgatc aggatgttcg ccgctttcaa aatacgtccg gaagagcggt
    24781 agttctgctc aagcttaatc accttcagcg ccggaaaatc ctgactcagc agcaccaggt
    24841 tttgcggacg tgcaccgcgc caggagtaga tcgactgatc atcgtcaccc accacggtaa
    24901 agcgcgcgcg gctgcccacc agcagtttca ccagttcata ctggctggtg ttggtgtcct
    24961 gatactcatc caccagcagg tagcgaattt tgttctgcca gcgctcgcgg acttcttcat
    25021 tgcgttgcag cagcaacgtc ggcaataaaa tcagatcatc gaagtcgaga acgttacagg
    25081 ctttcaggtg tgcatcatac agcccataac aatgggcaaa aatacgatcc cgctcgccct
    25141 ttgcctctgc cgccgcctgc gccggagttt tgagatcgtt cttccagtta gagatggtcg
    25201 aaatcagctg ttgcaggaga actttgtcat cttcaatcag cccctcggtc aactctttaa
    25261 gcaaagcaag ctgatcggta tcgtcaaaca gcgaaaagtt cgctttcatc cccaacgccg
    25321 catactcacg cttgatgata tccagcccca gcgtgtggaa ggtggagatc atcagcccac
    25381 gcgcctcttt gcgccccagc gtctgcccga cacgctcttt catctcgcgc gccgccttat
    25441 tggtaaaggt cactgccgcg atatgtcgcg cctgataacc gcaaccgcgg atcagatggg
    25501 cgattttatt ggtgatcaca cgagttttac cggaacccgc gcccgccagc accaggcagg
    25561 ggccggtaac gaattcgaca gcttgttgtt ggccggggtt tagacgcata ggtgtattgc
    25621 tcaatcttcg aacgggggga ggattgtagc agaatcgcat ttgaatgtga ccgcgtgtaa
    25681 gccgctaacc ggttggctgc ggatgtttaa atcagagaga gaagaccgaa atcggagttc
    25741 atcgtgaatc cattagactt agaaaatatc gggtcgtaac gttgtgttat tactttttca
    25801 gagtttacta aggagatgtt tactcgtggc gattaatttc agccctaagg ttggtgaaat
    25861 actggaatgc aactttggta actatcctgt tagccaaaat ggaccgttca gcacgacgta
    25921 ttacgatggc cgcattcctc ctgaaatgat aaaaaacagg ttagttgtcg tgctcaacgg
    25981 aaaaattaat ggcaacgcat gcatcgtcgt tccattatca accactcgcg accatgacaa
    26041 gttaaaacgt ggaatgcatg ttgaaatagc cagtaatgtt atcaatgacc tgcaattctt
    26101 tgatcagcag attcgttggg ccaaagccga cctggtacaa caagtcagtc gaaacagatt
    26161 aaacagagca agaacttaca gagggtatct taatcaatgt ttgccacatg agttggttgc
    26221 agacatacag agggcggtca tcaagtcaat caatgccata tcgctcataa attgacaata
    26281 aaatcaccat ccccgataat gactttgtag cccatagagg ccctaagttt tctccccttg
    26341 agggacctgc gaagcccgga ttttccgggc ttttgccata tatgtaccag cccctccctg
    26401 actgacaatt acgttagcat ctgcggatca tgctaaaatc gccgcctgac aattattcac
    26461 tcaaggcacg atcatggcaa aaacagcagc agcactgcat atccttgtaa aagaagagaa
    26521 actggctctg gatcttctcg agcagattaa gaacggtgcc gatttcggca agctggcgaa
    26581 gaaacactcc atttgcccat caggcaaacg cggcggtgat ttaggtgaat tccgccaggg
    26641 tcagatggtt ccggcgttcg ataaagttgt gttctcttgt ccggtactgg agccgactgg
    26701 cccgctgcat acccagttcg gttaccacat cattaaggtg ctttaccgta actaatagca
    26761 aggccttctc cgggagaagg ccttgaactc tgaatcgcta ttaacccgca acagcaatgc
    26821 gtttcatatc tgtcatatag ccgcgcagtt tcttacctac ctgctcaatc gcatggctgc
    26881 gaatcgcttc gttcacatca cgcagttgcg cgttatctac cgcgccttcc ggaatagctt
    26941 tacccaggtc gcccggttgc agctctgcca taaacggttt cagcaacggc acacaagcgt
    27001 aagagaacag atagttaccg tactcagcgg tatcagagat aaccacgttc atttcgtaca
    27061 gacgcttacg ggcgatggtg ttggcaatca gcggcagctc gtgcagtgat tcataatatg
    27121 cagactcttc aatgatgccg gaatcgacca tggtttcgaa cgccagttca acgcccgctt
    27181 tcaccatcgc gatcatcagt acgcctttat cgaagtactc ctgctcgccg attttgcctt
    27241 catactgcgg cgcggtttca aacgcggttt tgccggtctc ttcacgccag gtcagcagtt
    27301 tcttatcatc gttggcccag tccgccatca tgccggagga gaattcgccg gagatgatat
    27361 cgtccatatg tttctggaac agcggtgcca tgatctcttt cagctgttca gacagcgcgt
    27421 aagcacgcag tttcgccggg ttagagagac ggtccatcat cagggtgatg ccgccctgtt
    27481 tcagcgcttc ggtgatggtt tcccagccga actgaatcag tttttctgcg tatgccgggt
    27541 cggtgccttc ttccaccagc ttgtcgaagc acagcagcga gccagcctgc aacataccgc
    27601 acagaatggt ttgctcgccc atcaggtcag atttcacttc cgcaacgaag gacgattcca
    27661 gcacgcccgc acggtgacca ccggttgctg ccgcccatgc tttagcgatc gccatacctt
    27721 cgcctttcgg atcgttttcc gggtgaacgg cgatcagcgt cggtacgccg aatccacgtt
    27781 tgtactcttc acgtacttcg gtgcctgggc atttcggcgc aaccatgact acggtgatat
    27841 ctttacggat ctgctcgccc acttcgacga tgttgaaacc gtgcgagtaa cccagcgccg
    27901 cgccgtcttt catcagtggc tgtacggtgc gcactacatc agagtgctgc ttgtccggcg
    27961 tcaggttaac caccagatcc gcctgtggga tcagttcttc gtaagtaccc actttaaaac
    28021 cattttcggt cgctttacgc caggacgcgc gcttctcggc aatcgcttct ttacgcagag
    28081 cgtaggagat atcgagacca gaatcacgca tgttcaggcc ctggttcaga ccctgtgcgc
    28141 cacagccgac gatgactact tttttaccct gaaggtagct cgcgccatcg gcgaattcat
    28201 cgcgccccat aaagcgacat ttgcccagct gtgccagctg ctggcgcaga ttcagtgtat
    28261 tgaagtagtt agccatggtg attcctcgtg atgttgtgct tcttattgtt cggttcgcgt
    28321 ttgcgagatt gaattcacta tatgacagga aatttattgc ggaaattgat atattcacaa
    28381 cgtcacattg caatttctgc aacgtcaaca ttgagggctg cgccattgga tttacgcgat
    28441 ctgaaaacct tcctgcatct ggcggaaagc cgccattttg gccgcagcgc gcgggcgatg
    28501 cacgttagcc cgtccacgct ctcacggcag attcagcgcc tggaagaaga tctcggtcag
    28561 ccgctgtttg tgcgcgataa ccgcacggtg acgctgactg aagcgggcga agagctgcgc
    28621 gttttcgccc agcaaacgct gttgcagtat cagcagctgc gccacaccat cgatcagcaa
    28681 gggccgtcgc tctctggcga attacatatc ttctgctcgg tgactgctgc ctacagccat
    28741 ctgccgccga ttctggatcg cttccgcgcg gaacacccgt cggtggagat taaactcacc
    28801 actggtgatg cggcagatgc aatggaaaag gtggtcactg gcgaagcgga tctggcgatt
    28861 gccgggaagc cggagactct gcctggtgcg gtggcgtttt cgatgctgga gaacctggcg
    28921 gtggtgctga tcgctcccgc gttgccatgc ccggtgcgta atcaggtctc ggtagagaag
    28981 ccggactggt caacggtacc gtttatcatg gccgatcagg ggccagtacg ccgccgcatt
    29041 gaactgtggt ttcggcgcaa taaaatcagc aacccgctga tttacgccac ggtcggtggg
    29101 catgaagcga tggtatcgat ggtggcgctc ggctgtggtg tggcgctgtt gccggaagtg
    29161 gtgctggaaa acagcccgga gcctgtacgc aaccgggtaa tgattttaga tcgcagcgat
    29221 gagaaaacgc cgtttgaact gggcgtgtgt gcacaaaaaa agcggctgca tgagccgctg
    29281 attgaggcgt tctggaggat tttgccgaac cacaaatgac gttgtcgcgc gagtaggcct
    29341 gataagcgaa gcgctatcag gcatttttcc ctaacccgcc aaaaagaacc tgaacgccgg
    29401 gttattggtt tcgtcgtggc aatcgtagcc cagctcattc agccgggttt cgaaatccgg
    29461 ttcatggtcg ccaagttcga acgccgccag tacacgcccg tagtcggtac cgtgactgcg
    29521 ataatggaac agagaaatat tccagtgcgt acccagcgtg ttgaggaagc gcagcagcgc
    29581 acccggcgat tccggaaatt cgaagctgta gagacgttcc tgcaacggat gcgatggacg
    29641 ccccccaacc atatagcgca cgtgtaactt cgccatttcg tcgtcggaga gatcaaccac
    29701 gctgtagccg ccgtcgttga gcatctgcaa aatttctttg cgctcttcga ggccacggct
    29761 taagcgcaca ccgacaaaga tgcaggcgtt tttggcatcg gcaaagcggt agttgaattc
    29821 ggtgaccgaa cgtccgccaa gcagttggca gaatttgagg aagctacctt tctcttccgg
    29881 aatggttact gccagcaacg cttcacgctg ttcacccagt tcacaccgtt ccgagacgta
    29941 gcgcaggccg tggaagttca cgttggcacc ggaaagaata tgcgccagcc gttcgccgcg
    30001 aatgttgtgc tgggcgatat attttttcat tcccgccagc gccagcgcgc cagagggttc
    30061 cgccaccgcg cgcacatctt cgaataaatc cttcatcgcc gcacagatcg catcactatc
    30121 gacggtgatg atatcgtcga gatactcctg gcacaaacgg aaggtttcgt cgccgatgcg
    30181 ttttaccgca acgccttcag caaacagccc cacgcgcggc agatcaaccg gatgacccgc
    30241 atccagcgct gctttcaggc aggcggagtc ttccgcttct acggcgatta ctttgatttg
    30301 cggcatcagt tgtttgatca gcaccgccac gccagcagcc agaccgccgc cgccgactgg
    30361 cacaaatacg cggtcgagat gggcgtcctg ctggagcagt tccagcgcca gcgtaccctg
    30421 cccggcgatc accatcggat ggtcgaacgg cggcacccag gtgaacccct gctgctgcga
    30481 cagttcgatt gctttggctt tcgcttcatc aaagttcgca ccgtggagca gcacttcgcc
    30541 gccgaagccg cgtaccgcat cgactttgat gtcagcggta gcggttggca tgacgatcag
    30601 cgccttcaca cctaaccgcg cagaagaaaa cgcgacgccc tgcgcgtggt tacccgcaga
    30661 agcagtgatc acgccgtgcg ctttttgttc ttccgtcagg cccgccatca tggcgtatgc
    30721 gccgcgcagc ttaaagctgt gcactggctg gcgatcttcg cgcttcacca gaatgacgtt
    30781 atcaagacgc gacgacagtt tttccatttt ttgtagcggc gtaacctgcg ccgcctcgta
    30841 aaccggcgcg cgcagcaccg ctcttaaata ttcggcacct tccggagcac cggacagggg
    30901 ttgcgagtca gccattatta accccccagt ttcgatttat cgcgcaccgc gcctttgtcg
    30961 gcgctggttg ccaggctggc ataagcacgc agggcaaagg agacctgacg ttcacgattt
    31021 ttcggcgtcc aggctttgtc accccgagct tcctgcgctt cacgacgcgc cgccagttcg
    31081 gcatcgctta cctgtaactg aatgccacgg ttcggaatgt cgatggcgat caggtcgccg
    31141 tcttcaatca ggccaatgct gccgccgctt gccgcttccg gtgagacgtg gccgatagaa
    31201 aggccagagg tgccgccaga gaaacgaccg tcggtgatca gcgcacaggc tttaccgagc
    31261 cccattgatt tcaggaagct ggttgggtag agcatttcct gcatccccgg accacctttt
    31321 gggccttcat agcgaattac taccacatct ccggcgacaa ctttaccgcc gagaatcgct
    31381 tctaccgcgt cgtcctggct ttcgtatact ttcgccgggc cggtgaattt gaggatgctg
    31441 tcatcgacgc ccgcggtttt aacgatgcag ccgttttctg cgaaattacc gtagagcact
    31501 gccaggccgc cgtctttgct ataggcgtgt tccagcgagc ggatacagcc attggcgcga
    31561 tcgtcgtcca gcgtatccca acggcaatct tgcgagaatg cctgggtggt acgaatgccc
    31621 gccgggccgg cgcggaacat attttttacc gcgtcatcct gggtcagcat aacgtcgtat
    31681 tgttccagcg tttgcggcaa cgtcaggcca agtacgtttt tcacatcacg gttcagtaac
    31741 cccgcacgat ccagctcgcc gagaataccg ataacaccac cagcacggtg aacatcttcc
    31801 atatggtatt tttgggtgct cggcgcgact ttgcataact gcggaacctt gcgggaaagc
    31861 ttatcgatat cactcatggt gaagtcgatt tccgcttcct gtgccgccgc cagcaggtga
    31921 agtacggtgt tggtagagcc gcccatcgcg atatcgagcg tcatagcgtt ttcaaacgcc
    31981 gccttactgg cgatattacg cggcagtgca ctatcgtcgt tttgctcgta gtaacgtttg
    32041 gtcaattcaa caatgcgttt accagcattg aggaacagct gcttacggtc agagtgggtt
    32101 gccagcagcg agccgttgcc cggctgcgac agacccagcg cttcggtcag gcagttcatt
    32161 gagttagcgg taaacatccc ggagcaggaa ccgcaggttg ggcatgcgga acgttcaacc
    32221 tgatcgctct gggagtcaga tactttcggg tctgcgccct ggatcatcgc atcaaccaga
    32281 tcgagcttga tgatctgatc ggacagtttg gttttcccgg cctccatcgg gccgccggaa
    32341 acaaagatca ccggaatatt caggcgcaga gaggccatca gcatccccgg ggtgattttg
    32401 tcacagttag agatgcagac catggcgtcg gcgcagtggg cgttgaccat atactcaacg
    32461 gaatcagcga tcagttcgcg agatggcagt gaataaagca tccccccgtg gcccatggca
    32521 atcccatcat ccaccgcaat ggtgttgaac tctttggcaa cgccgccagc cgcttcaatt
    32581 tgttcggcga ccagtttacc gagatcgcgc agatggacgt gacccggtac aaattgggtg
    32641 aacgagttca caaccgcgat aatcggctta ccgaaatcgg cgtcggtcat tccggtggcg
    32701 cgccacagcg cacgagcacc cgccatatta cgaccatgag tggtggtggc ggaacggtac
    32761 ttaggcatac tttatttact cccagtgtct gtctcgtaaa tgggacggtg cgtgccgtcc
    32821 cattttttgt atttattgat taacttgatc taaccagccc catttatctt cggtttcgcc
    32881 ggtaaagagg ccgaagaagg cttgctgaat gcgtttggtg accgggccac aacggccttc
    32941 gccaacctga ataccgtcta cactgcgcac tggcgtgatt tctgccgccg taccggacat
    33001 aaagacttca tccgccaggt acagggattc gcgcgacagc acctgctcac gcacttcaat
    33061 tcccagctct ttcgccagtt tgatgatggc atcacgggta atacccggca gcgcggagga
    33121 ggtgaacggt ggggtgaaca gcacaccatc tttcacttca aacaggtttt cgcctgcgcc
    33181 ttcagagata taaccgttca catccagcgc gataccttcc tgataaccgt ggcggcgcgc
    33241 ttcgctaccc accagcaggg aagagagata gttaccaccg gcttttgccg cagttgggat
    33301 ggtgtttggt gctgcgcggt tccaggagga aaccatcgca tcgatcccct gctccagcgc
    33361 ttctgcgccc agatacgctc cccacgggaa agcggcgata atcacatcgg ttgagtatcc
    33421 cgctggcggg tttacgccca tgccaacatc accaacgaag atcagcggac ggatataggc
    33481 gctggtgaga ttgtttttgc ggatcacgtc acgacaagct tccatcagct catcaatgct
    33541 ctgcgaaacc gggaagcgat agattttggc ggagtcatgc agacgctgca tatgctcacg
    33601 atggcggaat acaaccggtc ctttgtgcga gtcgtagcaa cggatgcctt caaaaaccga
    33661 ggtgccatag tgcagcgcgt gcgacatcac atgcaccttc gcgtcttccc agcgaaccat
    33721 ctccccattg aaccaaatgt aatcagcttt cttcgtggtc atttttatat tccttttgcg
    33781 ctcaggcgcg gatttgttgt gatgtggttg tgctctggca gatggcaacg tgtgcgacgt
    33841 ccaccagttt atttaactga ctaaacagta agtcgaccga ccgtgggctg gcaacggtca
    33901 attcgatatt tatattttgt gcatcgctgg cggcggccat attcattgag cagacgtgga
    33961 aaccacgatg acgcaccacg cgtaaaacac gttctaaggt ttctggattg aagcgagccg
    34021 atacattgac ctgatgttgc atcatgataa tttctccaac atttctgaat tactggcgcc
    34081 aggcggcacc agcggccaga cgttctcaag ttcgtcgatt gagacatgaa gcaggtatgg
    34141 cccatcactg ttcagcatgg tgtcgagtgc cgcttcaacc tggtctttac gggtgatgtg
    34201 ttggccaggg atgccgaagg cgctggctaa cataaggaaa tcggggttat cagtaagggt
    34261 ggtttcgctg tatcgttcct gaaaaaacag ttgctgccat tgtcgaacca tccctaaccg
    34321 ttggttatcg agtaagacga ttttcaacgg taactgcttg cgttttacgg tgcccagctc
    34381 ttgcacattc atcatgaaag agccgtcacc ggagatacag acaacggtat cgttcggtcg
    34441 cgcgacttgt gcgccaaccg ccgccggtaa accaaaaccc atggtaccta agccgctgga
    34501 ggtgatgaaa ttttccgggc gagtgtgggc gatgtgctgc gcagcccaca tctggtgctg
    34561 ccccacatct gtggtcacga cgcaatccgc aggtttacga tccgacagtt gttttaacaa
    34621 caacggcgcg tagatagcgt caccgggatg gtcgtaacgc caggcatgtt catcacgcag
    34681 ctgcgcgcag tgttgctgcc agtcattgat atttaatggc tgctgtaatg ctggtaacag
    34741 cgcatttaaa tcaccctgta atgccacatg tgcctgacgc agcttgttca tttctgccgg
    34801 gtcgatatcc atatggataa cactggcgtg tggtgcgaag gtgttcaatt tgccggtcac
    34861 ccggtcatca aaacgtgcac ccacggcgat cagcaagtcg cactcctgca ccgcgaagtt
    34921 cgccgctttg gtgccatgca ttcccagcat gcccagatag tacggataat ctgcttcaac
    34981 tgcgcccagc cctttcagcg tgcaggtggc aggcattttt gtggtagcga gaaattctcg
    35041 taaagcagga actgcctgcg ccatacccac gccaccacca acgtacagca tcggtttttg
    35101 cgcttttgcc agcatctggc gcgcttgctc aacttcggca tgtgggaaag tcacttcgtt
    35161 ttcaacggtg gtgaaccacg gttcaaggtc accgctggct aactggatat cttttgggat
    35221 atcgaccaga accggaccag gacgacctga gctggcaacg tcgaatgctt cagccataat
    35281 gcgcggcaac tcttccagcg actgcaccag aaagctgtgc ttggtacagg ctaacgacaa
    35341 tcccaggaca tccacttcct gaaatgcgtc agtgccgata aacggtgcgg acacttgacc
    35401 ggtgatggca acaacaggga tagaatctaa cagtgcgtcc gcaagcccgg ttatcaggtt
    35461 ggttgcgccc ggaccagacg tggcgataca tacgccagtt ttgccggtag cacgggcata
    35521 accgatagcc gccattgccg caccctgctc atgtcggcac agcaagtgct ccacgccgcc
    35581 gtcatacaat gcatcgtaaa ccggcataat tgcgccaccc ggataaccga aaacggtatt
    35641 cacaccctgt gcccgcaacg catgtaccac ccactgtgcg ccattcatag ttagttcccc
    35701 gtcctgaaac ttgagaaaca gaattttgtg ctgttattca ttgtctgctc ctcggttatg
    35761 tttttaaggt caaaaaaaac ccccggacct ttcggtgcgg gggtcttagt tcgttaaggc
    35821 ttgatctcta agcctttcct cgtccaagtg cagccccgca cggtgggata ataatcacca
    35881 ccacgctaat cacgaccagg ctaatcactc gtagaagggc tgtcattttg tcttttcttg
    35941 catcttgttc gaaggaatgc ctaaagagtt accatagatt ttgcgaatag cacaagatat
    36001 tttttggccg ttttttatga tggctttaat aatttcaata gaaattgttg ttttttaaaa
    36061 agaaaatagt gagtgaaaaa aattcaattt cagtacgaat gttgttccct ccctcgtagt
    36121 tcatcctttg tccgctgcat ttttgcgagc atcattccac cgccagaaca actcaacttc
    36181 acttgcacaa ataaacagga atcagccact aaaattcacg aattagcggg ctacagcagc
    36241 gatatcttat gcacataatg gtgactcaag gagggcttat gtcactgtca attgttcata
    36301 cccgcgcagc cctgggagta aatgcgcccc caatcactgt tgaggtacat atcagtaaag
    36361 gtttgcccgg cttaacgatg gtgggcttac cagaaacaac ggtaaaagaa gctcgcgatc
    36421 gcgtgcgcag cgccattatc aatagcggat atgaatatcc ggcgaaaaaa atcaccatta
    36481 accttgctcc agccgatcta ccaaaagaag ggggacgata tgatttacct atcgccattg
    36541 cgttgctggc ggcctcagaa cagcttacag ccaataagtt agatgaatat gaattagtcg
    36601 gagaactggc gcttacaggc gctctgcgtg gcgttcccgg tgcaatctcc agtgcaactg
    36661 aagctattaa gtcgggcaga aaaattatcg tcgcgaaaga taacgaagat gaagtggggc
    36721 taattaacgg tgaaggatgc ctggtagccg atcatctgca agctgtctgt gcgtttctgg
    36781 aaggtaagca cgctctcgaa cgcccgaaac caactgatgc agtatcccgg gcgctacaac
    36841 atgatctcag tgatgttgtc ggtcaggagc aaggaaagcg aggactggaa attaccgccg
    36901 ctggcgggca caacctttta ctgattgggc cgccgggaac aggtaaaaca atgctcgcca
    36961 gccgtattaa tggtcttttg ccagatttaa gcaatgaaga ggcactggag agcgctgcga
    37021 tattaagtct ggtaaatgct gaatcagtac aaaaacaatg gcggcagcgc ccgttccgct
    37081 cacctcatca cagtgcgtcg ttaactgcga tggtgggcgg tggtgcaatt ccagggccag
    37141 gtgaaatttc gttggcgcat aacggcgtgc tttttcttga tgagctacct gaatttgaac
    37201 ggcgtacact ggatgccttg cgagagccga ttgaatccgg gcagatccat ctttcacgca
    37261 cacgagcaaa aataacctat ccagcccgtt tccagcttgt cgcggcgatg aatcccagcc
    37321 ctaccggaca ttatcaggga aaccataacc gctgcacgcc agaacagaca ttacgttatc
    37381 tcaaccggct ctcggggccc tttctcgacc gcttcgatct ctcactggag atcccattac
    37441 caccccccgg cattttgagt aaaacggtag tgccgggaga aagcagcgcc accgttaaac
    37501 aacgcgtaat ggccgccaga gagcgccaat ttaagcggca gaataaactg aatgcctggc
    37561 tggatagtcc ggaaatacgc caattctgca aacttgagag cgaagatgcg atgtggttgg
    37621 aaggaacact gatccatctg gggttatcga ttcgtgcctg gcagcggtta ttgaaagttg
    37681 cacgaaccat tgctgatatt gatcagtctg acattatcac acgtcagcat ttgcaggagg
    37741 cagttagcta tcgtgcgatt gaccgtttgc tcatccatct gcaaaaacta ctgacataaa
    37801 aaaagggcat ttcgcccttt ttattaatcg tcagaatcgg tgtagtcttc agcaccttca
    37861 acctgcggtt taccgccgga aagggtgtga aaacgttttg gacgcttgat acgcgtcata
    37921 tacttggacc acacgcgttc tgcttctgtc actggctcac gttcgccacg gcatactgct
    37981 acgaagagtt tctcttcctc ggtaaccggc tcgcgtttgc caagatccaa ctcattgaag
    38041 gcataaccat gacgctcaag cagttgtgcc tctttgatgg tgaaatcacc atgacgagag
    38101 aatccacgtg gataatgttt attgtcgaaa tatcgattag tcgtcgtaaa gctttccgcc
    38161 atcctgcacg ctcctaattc tttgaccgag ctagttatgg cgcggagtat tagttacgct
    38221 tgacagagtg taaaacaaaa catttaaatc ataacgacaa ataattttgc ggagagcact
    38281 gtggatacgg aattgttaaa aactttcctg gaagttagcc gaacgcgtca ctttggtcga
    38341 gcggctgaat cgctctatct gacccagtca gcagtgagct ttcgaatcag acaactggaa
    38401 aatcaactgg gtgtgaacct tttcacccgc cacagaaaca atatccgttt aaccgctgcc
    38461 ggtgaaaaac tactgcctta tgcagaaacg ctcatgagca cctggcaggc cgcccgtaag
    38521 gaggtggcgc atacctcacg acataacgag ttttctatcg gtgccagcgc ctcattgtgg
    38581 gaatgtatgc ttaatcagtg gctgggacgt ttgtatcaaa atcaggatgc ccatacaggc
    38641 ttacagttcg aagcgcgaat tgcccaacgg cagtctctgg taaaacagct gcatgaacgc
    38701 cagcttgatc ttcttattac cactgaagcg cccaaaatgg acgaatttag tagtcagttg
    38761 ctggggtatt tcactttagc gctttatacc agtgcccctt ctaaactaaa gggagatctt
    38821 aactatctgc gactggagtg ggggccagat tttcaacagc atgaggcagg tttgatcggt
    38881 gctgacgaag tgcctattct gacaaccagt tctgctgaac tggcacagca acagattgcg
    38941 atgcttaatg gttgcacctg gctacccgtc agctgggcgc gtaaaaaagg cgacctgcat
    39001 accgttgtcg atagcacaac actttcacgg ccgctttatg ccatatggct gcaaaatagc
    39061 gataaaaata cgttgattcg cgatctattg aaaattaacg tgctggatga agtgtattaa
    39121 tctgaatggc tggcaaggat gccggtagaa ggatttactt cagagagggt tatttcagat
    39181 aaaaaaaatc cttagctttc gctaaggatg atttctggca ggggcggaga gactcgaact
    39241 cccaacaccc ggttttggag accggtgctc taccaattga actacgcccc taattagggt
    39301 ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcaggt attctaaccg
    39361 actgaactac cgctccaccg aattctttta caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZFFG010000029        38344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFFG010000029 DAZFFG010000000
VERSION     DAZFFG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.029"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..923
                     /gene="ytfI"
                     /locus_tag="ACHJ7J_001898"
     CDS             <1..923
                     /gene="ytfI"
                     /locus_tag="ACHJ7J_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFO5000135.1"
                     /translation="HNNFIGAVELFVKSSYTKTHSNNFFNNIYHAFKKKDWISNYDSL
                     LTLREFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLRGSGFEY
                     EEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQAIGDVS
                     LGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIY
                     GGDESFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSDITKEK
                     ICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(986..1540)
                     /gene="ytfJ"
                     /locus_tag="ACHJ7J_001899"
     CDS             complement(986..1540)
                     /gene="ytfJ"
                     /locus_tag="ACHJ7J_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5000136.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQSIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1865..2071
                     /gene="ytfK"
                     /locus_tag="ACHJ7J_001900"
     CDS             1865..2071
                     /gene="ytfK"
                     /locus_tag="ACHJ7J_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO5000137.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2150..3493)
                     /gene="paeA"
                     /locus_tag="ACHJ7J_001901"
     CDS             complement(2150..3493)
                     /gene="paeA"
                     /locus_tag="ACHJ7J_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO5000138.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3816..4454)
                     /gene="msrA"
                     /locus_tag="ACHJ7J_001902"
     CDS             complement(3816..4454)
                     /gene="msrA"
                     /locus_tag="ACHJ7J_001902"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO5000139.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4660..6393
                     /gene="tamA"
                     /locus_tag="ACHJ7J_001903"
     CDS             4660..6393
                     /gene="tamA"
                     /locus_tag="ACHJ7J_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO5000140.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6390..10169
                     /gene="tamB"
                     /locus_tag="ACHJ7J_001904"
     CDS             6390..10169
                     /gene="tamB"
                     /locus_tag="ACHJ7J_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO5000141.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVNAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDITKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLSAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIVTLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10172..10513
                     /gene="ytfP"
                     /locus_tag="ACHJ7J_001905"
     CDS             10172..10513
                     /gene="ytfP"
                     /locus_tag="ACHJ7J_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO5000142.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10725..10976
                     /gene="chpS"
                     /locus_tag="ACHJ7J_001906"
     CDS             10725..10976
                     /gene="chpS"
                     /locus_tag="ACHJ7J_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO5000143.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10970..11320
                     /gene="chpB"
                     /locus_tag="ACHJ7J_001907"
     CDS             10970..11320
                     /gene="chpB"
                     /locus_tag="ACHJ7J_001907"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO5000144.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11400..11930)
                     /gene="ppa"
                     /locus_tag="ACHJ7J_001908"
     CDS             complement(11400..11930)
                     /gene="ppa"
                     /locus_tag="ACHJ7J_001908"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO5000145.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12240..13196
                     /gene="ytfQ"
                     /locus_tag="ACHJ7J_001909"
     CDS             12240..13196
                     /gene="ytfQ"
                     /locus_tag="ACHJ7J_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO5000146.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13336..14838
                     /gene="ytfR"
                     /locus_tag="ACHJ7J_001910"
     CDS             13336..14838
                     /gene="ytfR"
                     /locus_tag="ACHJ7J_001910"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO5000147.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSSEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIEMLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14852..15874
                     /gene="ytfT"
                     /locus_tag="ACHJ7J_001911"
     CDS             14852..15874
                     /gene="ytfT"
                     /locus_tag="ACHJ7J_001911"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO5000148.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15861..16856
                     /gene="yjfF"
                     /locus_tag="ACHJ7J_001912"
     CDS             15861..16856
                     /gene="yjfF"
                     /locus_tag="ACHJ7J_001912"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO5000149.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16889..17887)
                     /gene="fbp"
                     /locus_tag="ACHJ7J_001913"
     CDS             complement(16889..17887)
                     /gene="fbp"
                     /locus_tag="ACHJ7J_001913"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO5000150.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            18063..19436
                     /gene="mpl"
                     /locus_tag="ACHJ7J_001914"
     CDS             18063..19436
                     /gene="mpl"
                     /locus_tag="ACHJ7J_001914"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO5000151.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19592..20143)
                     /gene="yjgA"
                     /locus_tag="ACHJ7J_001915"
     CDS             complement(19592..20143)
                     /gene="yjgA"
                     /locus_tag="ACHJ7J_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO5000152.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20237..21589
                     /gene="pmbA"
                     /locus_tag="ACHJ7J_001916"
     CDS             20237..21589
                     /gene="pmbA"
                     /locus_tag="ACHJ7J_001916"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO5000153.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21773..22159
                     /gene="cybC"
                     /locus_tag="ACHJ7J_001917"
     CDS             21773..22159
                     /gene="cybC"
                     /locus_tag="ACHJ7J_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5000154.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(22204..22668)
                     /gene="nrdG"
                     /locus_tag="ACHJ7J_001918"
     CDS             complement(22204..22668)
                     /gene="nrdG"
                     /locus_tag="ACHJ7J_001918"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO5000155.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22858..24996)
                     /gene="nrdD"
                     /locus_tag="ACHJ7J_001919"
     CDS             complement(22858..24996)
                     /gene="nrdD"
                     /locus_tag="ACHJ7J_001919"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO5000156.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25389..27044)
                     /gene="treC"
                     /locus_tag="ACHJ7J_001920"
     CDS             complement(25389..27044)
                     /gene="treC"
                     /locus_tag="ACHJ7J_001920"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO5000157.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPEQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(27094..28515)
                     /gene="treB"
                     /locus_tag="ACHJ7J_001921"
     CDS             complement(27094..28515)
                     /gene="treB"
                     /locus_tag="ACHJ7J_001921"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO5000158.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28634..29581)
                     /gene="treR"
                     /locus_tag="ACHJ7J_001922"
     CDS             complement(28634..29581)
                     /gene="treR"
                     /locus_tag="ACHJ7J_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO5000159.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            29766..29819
                     /gene="mgtL"
                     /locus_tag="ACHJ7J_001923"
     CDS             29766..29819
                     /gene="mgtL"
                     /locus_tag="ACHJ7J_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO5000160.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29960..32656
                     /gene="mgtA"
                     /locus_tag="ACHJ7J_001924"
     CDS             29960..32656
                     /gene="mgtA"
                     /locus_tag="ACHJ7J_001924"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO5000161.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32862..33248)
                     /gene="ridA"
                     /locus_tag="ACHJ7J_001925"
     CDS             complement(32862..33248)
                     /gene="ridA"
                     /locus_tag="ACHJ7J_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO5000162.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33321..33782)
                     /gene="pyrI"
                     /locus_tag="ACHJ7J_001926"
     CDS             complement(33321..33782)
                     /gene="pyrI"
                     /locus_tag="ACHJ7J_001926"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO5000163.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDHRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33795..34730)
                     /gene="pyrB"
                     /locus_tag="ACHJ7J_001927"
     CDS             complement(33795..34730)
                     /gene="pyrB"
                     /locus_tag="ACHJ7J_001927"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO5000164.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34734..34868)
                     /gene="pyrL"
                     /locus_tag="ACHJ7J_001928"
     CDS             complement(34734..34868)
                     /gene="pyrL"
                     /locus_tag="ACHJ7J_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO5000165.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35149..35544)
                     /gene="yjgH"
                     /locus_tag="ACHJ7J_001929"
     CDS             complement(35149..35544)
                     /gene="yjgH"
                     /locus_tag="ACHJ7J_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5000166.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35675..36388)
                     /gene="bdcA"
                     /locus_tag="ACHJ7J_001930"
     CDS             complement(35675..36388)
                     /gene="bdcA"
                     /locus_tag="ACHJ7J_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5000167.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36459..37052
                     /gene="bdcR"
                     /locus_tag="ACHJ7J_001931"
     CDS             36459..37052
                     /gene="bdcR"
                     /locus_tag="ACHJ7J_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5000168.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            37197..37649
                     /gene="tabA"
                     /locus_tag="ACHJ7J_001932"
     CDS             37197..37649
                     /gene="tabA"
                     /locus_tag="ACHJ7J_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO5000169.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37772..>38344
                     /gene="yjgL"
                     /locus_tag="ACHJ7J_001933"
     CDS             37772..>38344
                     /gene="yjgL"
                     /locus_tag="ACHJ7J_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO5000170.1"
                     /translation="MSKISDLNYSQHITSADNFKPKNEVLNTWCVGMNDFARIAGGQD
                     SRRNILSPGAFLAFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSSKVIIEISLSTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDDS
                     SLHLITNKVLACYQREANKEIARLLNNHQKL"
BASE COUNT         9291 a         9461 c        10273 g         9319 t
ORIGIN      
        1 gacacaataa ttttattggt gcggtggagt tatttgtaaa gtcttcgtat acaaaaacac
       61 attcaaacaa tttttttaac aacatctatc atgcatttaa gaaaaaagat tggatttcga
      121 attatgatag cctgttaact ttgagggagt tctttcgttg cgcaactcag attgataaaa
      181 gtagttatca ggtgctttca tcaaaaaatg aaactgttaa cgccatggat aagtttctta
      241 tcagtttcag ccttaaagat aacggtgccg aatatacaat gacattgcga ggcagtggat
      301 ttgaatacga agaaataccc ataacaataa atgaatataa ctcattcatg gatttcaaaa
      361 accgcgagtt tccgttagaa caaaacagaa gattgtatgc ctgggatatt ttgcagaaaa
      421 aacagtctga cataccgaaa aggattaaag gttatattca ccaggcgatt ggtgacgtgt
      481 ctttaggtta tgccttattg gaggatatag taagcaaatt aaaacgaggt aaatttgaac
      541 tccaaatacc aggaggtgga attaaagaat gcgatggatg gtatatttac gaaaaaataa
      601 ttgatgataa ttttgccata gtcatagaat ctttgggctt tgcgcttaaa atctatggag
      661 gggatgaaag ttttcgtaat ggttcctctg ttgttttgga ggatgaagat tactctctta
      721 tttataattt tctggttaat gcaggttgcc aacaagtcga attagccgaa caagtggatg
      781 cgatagtaag tgcgaatctg gccgcagata gtgatattac caaagaaaag atttgcgaga
      841 aatataaatc gaccattgag gcgtttaaaa aagagcaact agcgttacca gtattagttc
      901 ggcgtaagaa ctcagagact taattcccct atcccataga taacgatagg ggaaaattat
      961 ttttgcagaa tttatgttat tgccattatt tgagcaactt ttgcagcagg tccatcactt
     1021 gctgcacctc ttcctgagtg agcgccccgt ctttggccca ttgcacgcga ccatccttat
     1081 ccagcaccac tacagcggaa ctttcttcat ccagctgcca ggcaccgcgc gcgacgccat
     1141 tgctatcgac aatagactgc gaccagggat aaagcttttt attactctcc agactgctgc
     1201 gcacaaacat gccggaaccc ggaatcgcgt cgtcggtgtt aacaatggtg gtggtctggt
     1261 aacgatcgtg cggtaacttc gctgatttaa tcgcttcaat cagcgtcgcg tttttctctt
     1321 ttgcagaggt gcgaccagca atatgttgca gtactcgcac ttttcccact aactgcgcgc
     1381 tgttccaggt tttgtagcta aactgatctt tatcaagcac caactcgccc cgatcggtaa
     1441 tgccaatcgg cggcactcgc tgaccggttt cgaactgatg tgcggaagcc atcatcggca
     1501 acagcaggca ggtgagtgcc agaatcttgc gtagggtcat ggtgtttcct tcttatgata
     1561 tgcaggtgat ccgaccactt gggtctgagt tttaatcata tgtgctattt atcgaatttc
     1621 ccgcaagtgt gatgccagtt tgcggtcaag cgcacaaatc atatgaaaaa tgaatgctta
     1681 tactgaagac cgcgcttcgg taaaaagata attctgaata attgtaacct ttaggtaaaa
     1741 aaagttatac gcggtggaaa cattgcccgg atagtctata gtcactaagc attaaaattt
     1801 gcgcctcata atagttgggc cgattgtggc accgcacagg cgtaatactc agcaggagat
     1861 aacaatgaaa attttccaac ggtacaaccc acttcaggtg gcgaagtacg taaagatcct
     1921 gttccgtgga cggttataca tcaaggacgt tggcgctttt gaatttgata agggtaagat
     1981 tcttatccca aaagtgaagg ataagctgca tttgtcagtg atgtccgaag ttaaccgtca
     2041 ggttatgcgt ttgcaaacag agatggctta accaaagtgc tatgcagtaa aaagtgctat
     2101 gcagtaataa gacggctcct gattcaggag ccgttgatgt ttctgggggt tacgcgacgc
     2161 tttcttcttt atctttagcg tcaggcagtt ttggcgaaag ggcggtggcc ttgctgtcga
     2221 tccgggtcac cagcagctgg tcgatgcggt agttatcgat atccaccact tcaaatttgt
     2281 agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt acgcagcata aacatcataa
     2341 agccgccgat ggtttcgtag ttgcccgact gcgggaattc gtcaatatcc agcacgcgca
     2401 tgacgtcgtc aatcggggta ccgccgtcaa tcagccatga gttctcatca cgggcgacaa
     2461 tctgctcttc cagcccctga ccgaccagat cgcccatcag cgtggtcatc acgtcgttga
     2521 gggtgatgat ccccaccacc agcgcgtact cgttcatgat caccgcgaag tcttcacctg
     2581 cggttttaaa actttccaac gcctctgaaa gggttaacgt atccggcaca atcagcgtgt
     2641 tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt agccagcacg cggttcagca
     2701 ggtctttaga atcgacatag ccgatgatgt ggtcaatatc ttcattacag acgaggaact
     2761 tagagtgcgg atgttccgcc accttattct tcagactttg ctcatcttcg tggagatcaa
     2821 accaaatcac gttttcacgc ggtgtcattg aagacggaac ggtacgggat tccagctcaa
     2881 agacgttttc aatcagctcg tgttcctgtt tacgtaacac gcccgccagc gcaccggctt
     2941 ccactaccgc gtagatgtca tcagaagtga tgtcatcttt acgtaccatt ggcagtttga
     3001 aaatacggaa gatcatgttc gccaggccgt tgaagaacca caccagcggg gtgcaaacgt
     3061 acaggcagaa gcgcatcggg ttgatgatac gcaaagccac cgcttctggc gcaatcatac
     3121 cgatgcgttt cggggttaaa tccgcaaaca ggataaacat gccagtcact aacgagaaag
     3181 agagaataaa gctcagttgc tcagagagct ctgccgacat atagcgggag aacaggctgt
     3241 gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc gagaatcgcc actgcgttca
     3301 gaccgatttg gaccacggta aagaacatgc cgggattttc ctgcatattc agaacgcgtt
     3361 gggcatttat attgccttca tcagccagca gtttaagttt gattttgcgt gaggcggcaa
     3421 gcgagatctc agacatcgag aagaacgcac ttacagcgat caagcagagt atgactaaaa
     3481 tactgtttaa catatcttat ccgacctttc aggccagatc ctcggaaggg gaagttgatt
     3541 aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa gtgaaccggc attttcagtg
     3601 gctagtatag cgtaaggtac tgtaaagtcg ccagagggtt aaaattcaga ctgctgataa
     3661 atgcaatact gcctgatgcg ctatgccgat caggcctacg cagtccttgc aatatattga
     3721 atttggacga ttttgtaggc ctggcaaggc gtttacgccg catccggcat aaataaaacg
     3781 cactttatca acaatctaca tttgtacccg taacgctatg cttccggtgg cagacagacg
     3841 ccaattccgc caattccaca gtaaccatac gggtttttat gcagatattg ctggtggtca
     3901 tcttcggcat aataaaacgg tgtggcgtta gcgatttccg tggtgatgtg acgatcgtca
     3961 tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg cgcgagctgc ggcatcctgt
     4021 tctggggtca gcggataaat cgctgaacga tactgcgtgc cgtggtcatt gccctgacgc
     4081 attccctggg cgggatcgtg attctcccaa aatacctgta gcaactgctc atagctgatg
     4141 acggaaggat cgtaaacaat gcgtaccgct tcggcatgac ccgtatcacc ggagcacact
     4201 tcccgataag tcggatttgg cgtgtagccg ccggtatagc ctgcggcggt gctgtaaacg
     4261 ccgggtaact gccagaacag acgctccaca ccccagaaac aacccatcgc aaaaatggca
     4321 atctccattc cgtcaggtac attggtcatt gagtgaccgt tgaccgcatg cagcgtggct
     4381 acgggcatcg gggtgttacg tccaggcagg gcatcggcgg gggaaaccag atgcttttta
     4441 tcaaataaac tcatggtgtc gctctcccga aaatcggtca ttggggttaa ggttgtaaca
     4501 agaggcgtat ttgcacacaa taaccaccgt gaataggtct aaagtaaaac ataagaaata
     4561 tttggggttt agtctgcttt ttaatccata ttactggatt tttgttaagc cgtttaacgg
     4621 cgttccaggg gcaggaaaaa aggatattca ggagaaaatg tgcgctatat ccgacagtta
     4681 tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg ccgcgaacgt ccgtctacag
     4741 gtcgaggggt tatcgggaca gctggaaaag aacgttcgtg cgcagctttc tacgattgaa
     4801 agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg tcgatgatgc tatccgcgaa
     4861 ggtctgaaag cgctgggtta ttaccagccg accattgaat ttgatctccg tccaccgcca
     4921 aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag gcgtgccggt gttaattggc
     4981 ggcaccgatg tggtattgcg cggcggcgcg cggaccgata aagactattt gaaattgctc
     5041 gatactcgcc cggctattgg cacggtactg aaccagggcg attatgaaaa tttcaaaaag
     5101 tccttaacca gcattgcgtt gcgtaaaggt tatttcgata gcgaatttac caaagcgcag
     5161 ctgggcattg cgctcggcct gcataaagcc ttctgggata ttgattataa cagtggcgaa
     5221 cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa tccgcgatga atacctgcaa
     5281 aatctggtgc cgtttaaaga gggcgatgag tacgaatcga aagatctggc agagctgaac
     5341 cgtcgacttt ctgctaccgg ctggtttaac tcggtcgtag tggctccaca atttgataaa
     5401 gcgcgcgaaa cgaaagtatt accattgacg ggcgtggttt cgccgcgaac tgaaaacacc
     5461 atcgaaaccg gggtcggtta ctctacggac gtgggaccgc gcgtgaaagc gacgtggaaa
     5521 aagccgtgga tgaactctta tggtcacagt ctgaccacca gtaccagtat ttccgcgccg
     5581 gaacagatcc tcgacttcag ctataaaatg ccgctgctga agaatccact ggaacaatat
     5641 tatttggtgc agggcggttt taagcgcact gacctgaacg ataccgaatc tgactccact
     5701 acgctggtgg cttctcgcta ctgggatctc tccagcggct ggcagcgtgc cattaacctg
     5761 cgctggagtc tcgaccactt tactcagggt gaaattacca acaccacgat gctgttttat
     5821 cctggggtga tgattagccg cacgcgttct cgtggtggcc tgatgccaac ctggggcgac
     5881 tcgcaacgct actctatcga ctactccaac acggcctggg gttcagatgt cgatttctcc
     5941 gttttccagg cgcagaacgt ctggatccgc acactgtacg atcgccatcg ttttgtgaca
     6001 cgcggcacgc tgggctggat tgaaaccggt gatttcgaca aagtaccgcc ggatctgcgt
     6061 ttcttcgccg ggggcgatcg cagtattcgc ggctacaaat acaaatctat cgccccgaaa
     6121 tacgccaacg gtgacctgaa aggggcctcg aagttgataa ccggatcgct ggaataccag
     6181 tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg atagtggcga agcggtaagc
     6241 gatattcgcc gcagcgactt taaaaccggt accggggtcg gcgtacgctg ggaatcgccg
     6301 gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg ataaagacga acacgggtta
     6361 cagttttaca tcggtctggg gccagaatta tgagtttatg gaaaaaaatc agcctcggcg
     6421 tggttatcgt tatcttactg ttgctgggat cggtggcgtt tctggtgggc accaccagcg
     6481 gcctgcatct ggtatttaaa gcggcggatc gctgggtgcc aggactggat attggcaagg
     6541 tcaccggcgg ctggcgcgat ctcaccttgt ctgacgttcg ttatgagcag ccaggcgtgg
     6601 cggtaaacgc gggcaatctg catctggctg tcgggcttga gtgcctgtgg aacagcagtg
     6661 tttgtattaa tgatctggcg ctgaaagaca tccaggtcaa tatcgacagc aaaaaaatgc
     6721 ccccttctga acaggttgaa gaagaggaag atagcggtcc gctggatctc tccacgccgt
     6781 atcccatcac cctgacacgg gtggcactgg acaacgtcaa catcaagatt gatgacacca
     6841 cggtatcggt gatggacttc acctccggcc tgaactggca ggagaaaacc ctgaccctga
     6901 aaccgacgtc gctgaaaggc ctgctgattg ctctgccgaa agtggcggaa gtggcgcagg
     6961 aagaagtggt cgaaccgaaa attgaaaatc cgcagccgga ggaaaagccg ctcggcgaaa
     7021 cgctgaaaga tctcttttct cgcccggtat tgccggaaat gaccgacgtg catttgccgc
     7081 ttaacctgaa cattgaagag tttaagggcg agcagctgcg cgtgacgggc gacacggaca
     7141 tcaccgtgag caccatgctg ctgaaagtga gcagcattga cggcaatact aaactggacg
     7201 ccctggatat cgattccagt caagggatcg tcaacgccag cggcacggcg cagctgtcag
     7261 acaactggcc ggtggacatc actctcaaca gtacactgaa cgtggagccg ttgaaaggtg
     7321 aaaaagtgaa gctgaaagtg ggcggcgcgc tgcgcgaaca gctggagatt ggcgttaatc
     7381 tttccggtcc ggtggatatg gatttacgcg cccagacgcg actggcggaa gccggattgc
     7441 cgctcaacgt ggaagtgaac agcaaacaga tttactggcc gttcactggt gagaagcagt
     7501 atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat gaccgattac acgctctcta
     7561 tgcgtacggc agtgaaggga caggagattc cgcccgcgac tattaccctc gacgccaaag
     7621 gtaatgaaca gcaggtcaat ctcgacaaac tcaccgtcgc ggcgctggaa gggaaaactg
     7681 aactcaaggc gttgctcgac tggcaacagg ccattagttg gcgcggtgag ctaacgctta
     7741 atggcattaa caccgccaaa gagttcccgg agtggccgtc gaaactcaat ggcttgatta
     7801 aaacccgcgg tagcctgtac ggcggcacct ggcagatgga ggtgccagaa ctgaagctga
     7861 ccggtaacgt caaacagaac aaagtgaacg ttgacggcac gctgaaaggc aacagttata
     7921 tgcagtggat gatccctgga ctgcatctgg aactcgggcc aaacagtgcc gaagtgaaag
     7981 gcgagctggg ggtaaaagat cttaatcttg atgccaccat caacgcgccg gggctggata
     8041 acgcgctgcc ggggcttggc ggtacagcga aagggttggt gaaagtacgc ggcacggtgg
     8101 aagcgccaca actactggca gatatcaccg cgcgcggcct gcgctggcag gaactttccg
     8161 tggcgcaggt tcgcgtggaa ggcgatatca aatccaccga tcagattgcg ggtaaactcg
     8221 acgtacgcgt tgagcaaatt tcgcagccgg atgtaaatat caacctcgtc accctgaatg
     8281 ccaaaggcag cgaaaagcag cacgagctac agttgcggat tcagggcgag cctgtctccg
     8341 ggcagcttaa tctggcagga agttttgatc gcaaagaaga acgctggaag ggaactctta
     8401 gcaatacccg cttccagacg ccggtcggcc cgtggtcgct gacccgcgat attgcgctgg
     8461 attaccgcaa taaggagcaa aaaatcagca tcgggccaca ctgctggctt aacccgaatg
     8521 cggaactgtg cgtgccgcaa actatcgatg cgggggccga agggcgtgcg gtggtgaatc
     8581 tcaaccgctt cgacctcgcc atgctgaaac cgtttatgcc agaaaccact caggccagcg
     8641 gtatcttcac gggtaaagcg gacgttgcct gggacatcac gaaagagggg ctgccgcagg
     8701 gcagtatcac tctttcgggg cgtaacgttc aggtaacgca aaccgtcaac gatgcggcgc
     8761 tgcccgtggc gtttcagacg ttgaatctgt cggcggagtt gcgtaacaac cgtgccgaat
     8821 tgggctggac catccgcctg accaataacg gccagtttga tggacaggtg caggtgaccg
     8881 atccgcaagg ccgccgtaat cttggtggca acgtcaatat ccgtaacttc aaccttgcga
     8941 tgataaaccc catctttacc cgtggggaaa aagcagcggg gatggtgagt gccaacttgc
     9001 gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca gcttcaggtt acgggtgtgg
     9061 atatcgatgg caactttatg ccgtttgata tgcaaccgag ccagcttgcg gtcaacttta
     9121 acggtatgcg ctcgacgctt gccggtacag tacggaccca gcagggtgaa atctacctga
     9181 acggtgatgc cgactggagc caaattgaaa actggcgggc gcgggtaacg gcgaaaggca
     9241 gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga tgtatcgcca gatgttgtat
     9301 tcgaggctac accaaacctg tttaccctcg atggtcgcgt ggatgtcccg tgggcgcgca
     9361 tcgtggtgca cgatctgccg gaaagcgcag taggcgtctc cagcgatgtg gtgatgctta
     9421 acgataacct gcaaccggaa gagccgaaaa cggcgtcgat tccgattaac agtaacctga
     9481 ttgtccacgt tggcaacaat gtgcgcattg acgcctttgg cctgaaagcg cggctgacgg
     9541 gcgatcttaa cgtcgttcag gacaaacaag ggctgggcct gaacgggcag atcaacatcc
     9601 ctgaagggcg cttccatgcc tatggtcagg atctgattgt gcgtaaaggt gagttactgt
     9661 tctctggccc gccggatcaa ccgtatctta atattgaagc tattcgtaac ccggatgcta
     9721 cagaagacga cgtaatcgcc ggagttcgcg tcactggtct ggcggacgaa ccgaaagcgg
     9781 agatcttctc tgacccggcg atgtcgcaac aagctgcctt gtcttatttg ctacgtggac
     9841 aagggctgga gagcgatcag agcgacagtg cggcaatgac ctcgatgctg attggtctgg
     9901 gggttgcgca aagtggccag attgtgggta aaatcggcga gacgtttggc gtaagcaatt
     9961 tagcgctcga cacccaggga gtaggcgact cctcccaggt agtggtcagc ggctatgtat
    10021 tgccaggtct gcaagtgaaa tacggcgtgg gtatatttga ctctatagta acactcacgt
    10081 tacgttatcg cctgatgcct aagctatatc tggaagccgt gtctggtgta gaccaggcac
    10141 tggatttgct ctatcagttc gagttttagc aatgcgaata tttgtctacg gcagtttacg
    10201 ccacaaacaa ggcaacagtc actggatgac caatgcccag ttactgggcg atttcagtat
    10261 cgataactac cagttgtata gcctgggcca ctatccaggc gcagttccgg ggaacggaac
    10321 ggtacacggt gaagtttatc gtattgacaa cgccacgctg gccgaacttg atgccttgcg
    10381 caccaggggc ggtgaatacg cgcgccagtt gattcagacg ccgtacggga gtgcatggat
    10441 gtacgtttat caacgacccg tcgatggatt aaagctaatt gaaagcggcg actggttaga
    10501 cagggataag taaccctatg catacgccac cttcgggtgg cgttgttttt tgcgagacga
    10561 ctcgcattct gttttgtaat tccctcacct tttgcttttc tctccgagcc gctttccata
    10621 tctattaacg cataaaaaac tctgctggca ttcacaaatg cgcaggggta aaacgtttcc
    10681 tgtagcaccg tgagttatac tttgtataac ttaaggaggt gcagatgcgt attaccataa
    10741 aaagatgggg gaacagtgca ggtatggtca ttcccaatat cgtaatgaaa gaacttaact
    10801 tacagccggg gcagagcgtg gaagcgcagg tgagcaacaa ccaactgatt ctgacaccca
    10861 tctccaggcg ctactcgctt gatgaactgc tggcacagtg tgacatgaac gccgcggaac
    10921 ttagcgagca ggatgtctgg ggtaaatcca cccctgcggg tgacgaaata tggtaaagaa
    10981 aagtgaattt gaacggggag acattgtgct ggttggcttt gatccagcaa gcggccatga
    11041 acagcaaggt gctggtcgac ctgcgcttgt gctctccgtt caagccttta atcaactggg
    11101 aatgacgctg gtggccccca ttacgcaggg tggaaatttt gcccgttatg ccggatttag
    11161 cgttccttta cattgcgaag aaggcgatgt gcacggcgtg gtgctggtta atcaggtgcg
    11221 gatgatggat ctacgcgccc ggctggcaaa gcgtattggt ctggctgcgg atgaggtggt
    11281 ggaagaggcg ttattacgct tgcaggcggt ggtggaataa ggtgtgttta tttatcgcgg
    11341 gcataaaaaa acccttacta accgaagccc ggcgttcagg gttattacgc cagaagaact
    11401 tatttattct ttgcgcgctc gaaggaggca acgatttcag ctttagcggc ttctgcgttt
    11461 tcccaacctt caactttcac ccacttgcct ttttcgaggt ctttgtagtg ctcgaagaag
    11521 tgagcgattt gcgctttcag cagttcaggc agatcgttaa cgtctttaat gtgatcgtat
    11581 tctttgctca gcttgctgtg cggaaccgca accagtttcg catcttcacc ggcttcgtcg
    11641 gtcattttca gaacgccaac cggacggcaa cggatcacag aacccggctg cagcgggtac
    11701 ggagttggga ccagtacgtc aactgggtca ccgtccagag acagggtgtg gttgatgtaa
    11761 ccgtagttgc atggatagaa catcgcggtg gacatgaagc ggtcaacgaa cagtgcgccg
    11821 ctctctttgt cgatttcgta tttgatcgga tctgcgttag ccgggatctc aataacaacg
    11881 tagatgtctt ccggcagatc tttacccgca gggacgttga gtaagctcat gtctgtttcc
    11941 tttaaaaata tgtggcaaac aagtgccgag tattatagcc aactcgcgcc gaatgtcttc
    12001 gcttgttttc gtcttcattt tcctttttca ccagttttaa gacggtatac agagcaggaa
    12061 aatccataac cgtagccgca tttttcatag tgagatgaaa gcgattacaa acttgtgatt
    12121 aacgttttat ttactttttt gaagtgtgat gtaacgcaat ctgttacata acgaattgtc
    12181 tatagttttt tcgcgaacat cttttaacca ataataacta ccccgacgag gacaacccta
    12241 tgtggaaacg cttacttgta gtctctgcag tctcggcagc catgtcgtct atggcgttgg
    12301 ccgctccatt aaccgtagga ttttcgcagg tcggatcgga atccggctgg cgcgccgcag
    12361 aaaccaatgt ggcgaaaagt gaggccgaaa aacgcggaat tacgctgaaa attgccgatg
    12421 gtcagcaaaa gcaggaaaac cagattaaag cggtacgttc cttcgttgca caaggggtgg
    12481 atgcgatctt tatcgctccg gtggtcgcga caggttggga accggtatta aaagaggcga
    12541 aagatgccga aatcccggtc ttcttgctcg accgttccat cgatgtgaaa gacaaatctc
    12601 tctatatgac caccgttact gccgacaaca tcctcgaagg taagttgatc ggtgactggc
    12661 tggtaaaaga agtgaatggc aaaccatgca acgtggtgga gctgcagggc accgttgggg
    12721 ccagcgtcgc cattgaccgt aagaaaggct ttgccgaagc cattaagaat gcgccaaata
    12781 tcaaaatcat ccgctcgcag tcaggtgact tcacccgcag taaaggcaaa gaagtcatgg
    12841 agagctttat caaagcggaa aacaacggca aaaacatctg catggtttac gcccataacg
    12901 acgacatggt gattggtgca attcaggcaa ttaaagaagc gggcctgaaa ccgggcaaag
    12961 atatcctcac gggttccatt gacggtgtac cggacatcta caaagcgatg atggatggcg
    13021 aagcgaacgc cagtgttgaa ctgacgccga acatggcggg acccgctttt gacgcgctgg
    13081 agaaatacaa aaaagacggc accatgcctg aaaagctgac gttaaccaaa tccacccttt
    13141 acctgcctga taccgcaaaa gaagagttag agaagaagaa aaatatgggg tattgagggt
    13201 tgctatgcct gatgccgatt cgtaggccgg ataaggcgct cgcgccgcat ccggcgatgg
    13261 tgcactgaag cctgatgcga cgcttaccgc gtcttatcat gcctactggg agcacgcttt
    13321 acaccggggg aaaccatgac gaccgaccaa caccaggaga tcctccgcac cgaaggatta
    13381 agtaaatttt tccccggcgt caaagcgtta gacaacgttg atttcagcct gcgccgtggc
    13441 gaaatcatgg cgctgctcgg tgaaaacggg gcaggaaaat caacgctaat caaagcatta
    13501 actggtgtat accacgccga tcgcggcacc atctggctgg aaggccaggc tatctcaccg
    13561 aaaaataccg cccacgcgca acaactcggc attggcaccg tctatcagga agtaaacctg
    13621 ctacccaata tgtcggtcgc tgataatcta tttataggcc gcgaacccaa acgcttcggc
    13681 cttctacgcc gcaaagagat ggaaaagcgc gccaccgaac tgatggcatc ttacggtttc
    13741 tccctcgacg tgcgcgaacc gctcaaccgc ttttcagtcg cgatgcagca aatcgtcgct
    13801 atttgccggg ctatcgatct ctctgccaaa gtgctgatcc tcgatgaacc caccgccagt
    13861 ctcgacaccc aggaagtgga gttactgttt gacctgatgc gtcagttgcg cgatcgcggc
    13921 gtcagcctga tttttgtcac tcactttctc gatcaggtct atcaggtcag cgatcggatc
    13981 accgtcttac gcaacggcag tttcgtaggc tgtcgggaaa cgtgcgagct accgcagatc
    14041 gaactggtaa aaatgatgct ggggcgcgag ctggataccc acgcgctaca gcgtgccggg
    14101 cgaacattgt tgagcgacaa acccgttgcc gcgttcaaaa attacggcaa aaaaggaacg
    14161 atcgcaccgt ttgatctcga agtacgcccc ggcgagatcg tcggtctggc tggcttgctg
    14221 ggatcaggac gtaccgaaac cgccgaagtg atcttcggta tcaaacctgc tgacagcggc
    14281 actgcgttga tcaaaggcaa accgcaaacc ctgcgatcgc cacatcaggc gtcggtactg
    14341 ggcatcggct tctgcccgga agacaggaaa accgatggca tcatcgccgc cgcctcggta
    14401 cgggaaaata tcatcctcgc tctccaggcc cagcgcggct ggctacgacc catttcccgc
    14461 aaagaacagc aagagattgc cgaacgcttt atccgccagc ttggcattcg cacaccttca
    14521 tctgaacaac cgattgaatt tctctctggc ggcaatcagc aaaaagtgtt gctttcacgt
    14581 tggctactga cccgaccgca atttctgatc cttgatgagc caactcgcgg cattgatgtt
    14641 ggtgcccacg ccgagatcat ccgcctgatt gaaatgctat gcgccgatgg tctggcgctg
    14701 ctggtgatct cctccgaact ggaagaactg gtgggctatg ccgaccgggt gatcatcatg
    14761 cgcgatcgca aacaggtggc ggagatcccg ctggcagagc tttccgttcc ggcgatcatg
    14821 aacgccattg cggcgtaagg agaacagtgt gatgcctcaa tctctcccgg acaccactac
    14881 gccaaaaagg cgctttcgct ggccaacggg aatgccgcag ctggcggcac tgttgctggt
    14941 gctgctggtc gatagcctgg tggccccgca tttctggcag gtggtgcttc aggatgggcg
    15001 tttgttcggt agccctatcg acattcttaa tcgcgccgcc cccgttgcgc tactggcgat
    15061 tggtatgacg ctggtgatcg caacaggtgg gattgatctc tccgtagggg cggtgatggc
    15121 tatcgccgga gccacaacgg ctgcgatgac ggtcgcggga ttcagcctgc cgattgtttt
    15181 gttaagcgcc ctgggcaccg gcatcctggc gggattgtgg aacggcatac tggtagcgat
    15241 cctcaaaatt cagccgtttg tcgccactct gatcctgatg gtcgccgggc gcggcgtggc
    15301 gcaactgatc acctccggac agatcatcac gtttaactcg ccggatctct catggttcgg
    15361 cagtggatcg ctgttgttcc tgccaacgcc ggtcattatc gcggtgctga cgcttatcct
    15421 gttctggctg ttgacccgca aaacggcgct ggggatgttt atcgaagccg ttggtatcaa
    15481 cattcgggcg gcaaaaaatg ccggagtcaa cacacgaatc atcgtcatgc tcacctacgt
    15541 gttgagcggg ctatgtgcgg cgattgcggg cattatcgtg gcggcggata ttcgcggtgc
    15601 cgatgccaat aacgccgggt tatggctgga gctggacgcc attctcgcgg tggtgattgg
    15661 cggtggttcg ctgatgggcg gacgttttaa cctactgctt tcggtggtgg gggcgctgat
    15721 tattcagggg atgaacaccg gaattttgct ttcgggcttt ccgccagaga tgaaccaggt
    15781 ggtgaaagcg gtggtggtgc tttgcgtgct gattgtccag tcgcaacgct ttatcagtct
    15841 gattaaagga gtgcgtagcc gtgataaaac gtaatttgcc actgatgatc accatcggcg
    15901 tctttgtgct gggttatctc tactgcctga cccagtttcc cggttttgct tccacaagag
    15961 tgatctgcaa tatcctgacc gataatgcct ttcttgggat aatcgccgtt ggcatgacct
    16021 ttgtgatcct ctccggtggg atcgatctct ctgttggttc ggtgatcgcc tttaccggcg
    16081 tgtttctggc aaaagtgatt ggcgatttcg gtctctcacc gctgctggcg tttccgctgg
    16141 tgctggtgat gggctgtgcc ttcggcgcat ttatggggct tttgatcgac gccctgaaga
    16201 tcccggcatt tatcattacc ctggcgggga tgttcttttt gcgcggcgtc agctacctcg
    16261 tttcggaaga gtcgattccg ataaaccatc ccatttatga cacgctctca agccttgcgt
    16321 ggaaaatccc tggcggcggt cgcttaagtg cgatgggact gctgatgttg gcggtggtgg
    16381 ttatcggcat attcctcgcg catcgtaccc gttttggtaa tcaggtatac gccattggcg
    16441 gcaatgcgac gtcggcaaac ctgatgggga tttctacccg tagcactact attcgcattt
    16501 atatgctctc caccggactg gcaacgctgg cggggattgt cttctcgatt tatacccagg
    16561 ccggatatgc gctggcgggc gtaggtgtgg aactggacgc tatcgcctca gtggtgattg
    16621 gcggtacgct tttgagcggt ggcgttggaa cggtattagg gacgcttttt ggcgtggcga
    16681 ttcagggact gattcagact tacataaact ttgatggcac gctgagctca tggtggacga
    16741 aaatcgccat cggtattttg ttgtttattt ttatagcatt acagcgtggg ttaacggtgc
    16801 tgtgggagaa tcgtcagagt tcgccagtga caagagtcaa cattgcgcag caataaaaac
    16861 gcctctccgt gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt
    16921 cgacatcttc aaccatatgg tcgttgccga caaagaatga acggcgctgg tgcagggttt
    16981 ccgggatgat atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg
    17041 ccaggaatgc catcgggttg cactcataca gtaaacgcag tttaccgtcc gggtggctgg
    17101 cggtgcttgg gtagagataa ataccgcctt tcagtaggtt acggtggaaa tccgcaacca
    17161 gtgagccgat ataacgtgag gtatacgggc ggttggtgga tttatcttct tcctggcaga
    17221 atttaatgta cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt
    17281 aggttttgcc tttctccggg aagcgcatcc gttcctggca caggcagaaa acgccgagcg
    17341 aaggatcgta agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg
    17401 agccgtatac cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt
    17461 ccgttaccgg cgtgccgacg ggcgtaactc ggcggtagat ggagaaaatg gtaccgacag
    17521 agacgttaac atcgatgttg gacgagccat ccagtgggtc catcagcacc acgtattttg
    17581 catgttcaca gccttcaaag acaacaatct catcttcttc ttcagaggca atgcccgcaa
    17641 cgatatcgcg tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagtttct
    17701 gttgaacctc gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg
    17761 ctttgttgat atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag
    17821 tgagctcacc ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg
    17881 ttttcataaa actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa
    17941 ataatctcaa tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg
    18001 cgtttctcac ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac
    18061 gtatgcgcat tcatatttta ggaatttgtg gcacgtttat gggcggtctg gcgatgctgg
    18121 cgcgccagtt aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca
    18181 ccttacttga gaagcaaggc attgaactga ttcagggtta cgatgccagc cagctcgatc
    18241 cgcagccgga tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag
    18301 cggtactgga aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc
    18361 tgcgcgaccg ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa
    18421 tggcgacctg gattctggaa cagtgtggtt acaaaccggg ctttgtaatc ggcggtgtgc
    18481 cggggaactt tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg
    18541 atgagtacga ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta
    18601 cgctgatcct caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga
    18661 tccagaaaca gttccaccat ctggtgcgta tcgttccggg gcagggtcgt attatctggc
    18721 cggaaaatga catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc
    18781 tggtgggtga gcagggtcac tggcaggcga aaaagctgac caccgatgct tctgaatggg
    18841 aagtcttgct ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggtc ggtgaacata
    18901 atatgcacaa tggcctgatg gcgattgcgg cggcgcgtca tgttggtgta gcgccggcag
    18961 atgccgctaa cgcgctgggt tcgtttatta acgctcgccg ccgtctggag ttgcgtggtg
    19021 aagcgaatgg cgtcacggta tatgacgatt ttgcccatca tccgacggcg attctggcaa
    19081 cgcttgcggc gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggagc
    19141 cgcgctcgaa taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc
    19201 gtgccgatga agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg
    19261 cagaagcctg cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg
    19321 tggtgaaaac cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg
    19381 ggatccatca gaaactgctg gatgggctgg cgaagaaggc ggaagccgcg cagtaattcg
    19441 gccttagcca gatatagtgt gcgtaggccg gataaggcgt tcacgccgca tccggcattt
    19501 gagcatagtg cctgatgcga cgcttgatgc gtcttatcag gcctacaggt gcaccgtatc
    19561 cggcaaacca tactcacatc aacaacgaaa attacccttc gttctccgct aactcgcgca
    19621 gatactggaa aatctggcgt gcggatttcg gcggcttatt cccttctttc tctttcttcg
    19681 cgttgcggat cagagtacgc agttgctgac gatcggcatc gggccacaga tttaatacct
    19741 cagcgatggc gtcatcacct tgatcgatca gacgatcgcg caggttttcc agtttatgga
    19801 acagcaccac ctgctggttg tgacggtttt tcagcttatc cagcgcctga cgaataggct
    19861 ctacgtcgcg ctggcgcagc atcttaccaa tgagctgcaa ctggcggcgg cgaccttcca
    19921 tcttaatacg ctgggcgagc tcaatagccg cgcgcagatc cgcatctaac gggatcttat
    19981 ccagcgcgtt tttccccaga tcaacaattt ccgcgccaag gcgttttagc tcctcggcat
    20041 cacgtttaat ttcactttta ctgacccaga taatttcatc gtcttcgtct tcgatgtcat
    20101 caccgggaac gtcgtcgagc cagtcttcgg gctgcttagt catctcaggc tccttaaaaa
    20161 aagaggctaa tgttaccagt taagatgcgc actgaaaaac ggttctctgt tagacttcag
    20221 agaaactctc tacattatgg cacttgcaat gaaagtaatc tctcaagttg aagcgcagcg
    20281 caagattctg gaagaagcag tttccactgc gctggagttg gcctcaggca aatcggacgg
    20341 tgcggaagtt gccgtcagca agaccaccgg cattagcgta agcacgcgtt atggtgaagt
    20401 ggagaatgtt gaattcaata gcgatggcgc gctggggatc actgtttatc accagaaccg
    20461 caaaggtagc gcatcatcca ccgatttaag cccgcaggcc attgcccgta ctgtacaggc
    20521 ggcgctggat attgcccgtt atacctcgcc agatccctgt gccggcgtgg cagacaaaga
    20581 gctgctggcc tttgacgcac cagatctcga cttgttccac cctgcggaag tttccccgga
    20641 tgaagccatt gaactggcgg cccgcgcaga acaggcggca ttgcaggcgg acaaacgcat
    20701 caccaatacc gaaggtggca gctttaacag ccactacggt gtcaaagttt ttggcaacag
    20761 ccacggcatg ttgcagggtt actgctcaac gcgtcattcg ctctccagct gtgtaattgc
    20821 cgaagaaaat ggcgatatgg agcgtgatta cgcctacacc attggtcgtg cgatgagcga
    20881 tctgcaaacg ccagagtggg ttggggccga ctgtgctcgc cgtactttat cgcgcctgtc
    20941 accgcgtaaa ctctccacca tgaaagcgcc ggtcattttt gccaatgaag tggcaaccgg
    21001 gctttttggc catctggtgg gggcgatagc gggtggatcg gtttaccgta aatctacctt
    21061 cctgctggat tcgctgggta aacaaattct gccggactgg ctgaccattg aagagcatcc
    21121 gcatctgctg aaagggctgg cgtcgacgcc attcgacagc gaaggtgtgc gcaccgagcg
    21181 tcgcgatatt attaaagatg gcatcctgac tcagtggctg ctgaccagct attcggcgcg
    21241 aaaactgggg ctgaaaagca ccggacatgc gggcggtatt cacaactggc ggattgccgg
    21301 acaaggtcta agcttcgaac aaatgctcaa agagatgggc accgggctgg tggtgacgga
    21361 gctgatgggc cagggcgtga gtgccattac cggtgattat tcccgtggtg cagcgggctt
    21421 ctgggtagaa aacggcgaaa ttcagtatcc ggttagcgaa atcaccatcg caggtaattt
    21481 aaaagatatg tggcgcaata ttgtcaccgt cggtaacgat attgaaacac gcagtaatat
    21541 acagtgtggt tctgtgctgt tgccggagat gaaaatcgcc ggacagtaaa aaaactggcg
    21601 cgacctgccg cgctataaac taacaaatta cacaaaatca ttcgcactgc atcgagacgg
    21661 aaagtgaatg agggccgggg agcgtactcg cagtacgtga cccggtcgaa tgagcgtagt
    21721 cgtcgatgag gcagtgtgaa ggatgaagtg taaataaaaa aggaagtgag caatgcgaaa
    21781 aagcctgtta gctattcttg cagtctcctc gttggtattc agttcggcgt cgtttgccgc
    21841 tgatcttgaa gacaatatgg aaaccctcaa cgacaattta aaagtggtcg aaaaagccga
    21901 taacgcggcg caagtcaaag acgcgttaac gaagatgcgc gccgcagcgc tggatgcgca
    21961 aaaagcaacg ccgccgaagc tcgaagataa atcaccggac agcccggaaa tgaaagactt
    22021 ccgccacggt ttcgacattc tggtcggtca gattgacgac gcgctgaagc tggcaaatga
    22081 aggtaaagta aaagaagcgc aggctgctgc agagcaactg aaaacgaccc gcaattcata
    22141 tcacaagaag tatcgttaat tcctcatttc cctgttgcct gcactcaggt aacagggaac
    22201 ttatcatcgc aaatgatgca ccacctgatt gctgctgccg cgccagatca gggaagggtc
    22261 ttttaaatcc tgcacaaatt tgccgtcgac cagcacgtta atcaaatcaa caacctgcat
    22321 ttgcgcagcg ttgagttcgt cgagtttata gcctgtccac acccagatgt ctttacccgg
    22381 acactcggcg cggatgcgtt gtaccagttt cagaatatcc ggcacgtttt gcggatgcag
    22441 cggatcgccg ccggagaggg aaataccctg gcgtttgata cgagtgtcat tcagatcgtt
    22501 aatgatctgg tcttccattg ctttagtaaa tggctgaccg gaatttaccc gccatgtgct
    22561 tttgttatag caaccggggc attcatgaac acacccggag acaaacaggg tgcagcgagt
    22621 gccgggaccg ttgacgatgt cgacaggata gtactgatga taattcatta tttcgcctgg
    22681 cttatcagtc cgcgtgtagg ctgtatcagg caatcaacgc ctgatgcgac gctgtcgcgt
    22741 cttatcaggc ctacagccgt tgccaaatgt aggccggata aggcgtttac gccgcatccg
    22801 gcaatcaacg cgggatgcga cgctttcaca tcacatccgg catttgctta gtgcagatca
    22861 acctatctgc ccatttccca aatgtttaac gcggcgctta acttcttcct gcttaccggc
    22921 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
    22981 acgggaggcg tcatggttac cacatttcgg gcaggtgaag cctttgctgg tgcactcgaa
    23041 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
    23101 tacatgctga tagctgtaat cccagacatc ttccagcgcc ttcaggttgt gctgaatgtt
    23161 tggatactcg ccgtagcaaa tgaaaccgcc gttcgccagc ggcgggtaag gcgcttcaaa
    23221 gtcgatcttg tcgtacgggt tcaccttctt ctccacatcg aggtggaaac tgttggtgta
    23281 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
    23341 gcggtcgcac aggttttcac tcggcgtgct gtagagactg aaaccataac ccgtttcttc
    23401 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
    23461 ctgctcgttg tcgtaaacat gttcgccgcc gaacagcgcg ttaatggttt cgtggatgcc
    23521 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
    23581 agcattgaga cgcacgccac aagcaccttc catatagagg atcggggcca cgcgcgcttt
    23641 cacgccttcg agacgagcga tacgggtcat cagcgcctta cgtgccagca ccagacgttc
    23701 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
    23761 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
    23821 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacttt
    23881 cactacctga tcgtagttca ggatatccgg atacatgcgt ttgcttgcgc actccagcgc
    23941 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
    24001 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
    24061 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
    24121 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
    24181 ctggaaggca tcgtagcact ctttaatggt acgagagtta gcgtagcctt cggcgtccgg
    24241 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtga caaacggtgc
    24301 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
    24361 aataatctgc gcagttacag cggttgccgt agagatcgac ttcggcggtt caatctcggc
    24421 gttccccatc ttaaagccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
    24481 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
    24541 ctgcaccacg tcacgcggca gcaggtgctg acgtgcatag tgtttagcca cgatcccggc
    24601 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
    24661 cgaggcgttg gtctgctcga ccaggccacg gatctcctgg ttcaggcgac cgcgtttttc
    24721 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
    24781 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
    24841 gccctgcatc tgctcgctga caaccgcggc aacagtggcg caataatcgg catcatcgac
    24901 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
    24961 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
    25021 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
    25081 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
    25141 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
    25201 gtgctttgca tcaaggatgt ttgaactttt tttgatgtag ctcaaagtaa aaagcagagc
    25261 gtacggatga cgggcgctac agcgatatgt aaatttttaa tgaatttgct ggttgaaaaa
    25321 tcaacaaaaa caacatgctg acagacaaaa ccccggaatg accggcatta ccggggctta
    25381 gggaagattt acttttgtaa ccaccagaca gcctcaaaag gccgtaaagt catggcacag
    25441 ggttgtggtg aggcttcttc gtagttatgc atcacaagct gccagttgcc gcgcatttgc
    25501 tctggctgcc agggttggat ctcacggcta agattggcaa tgaccagcaa ggtttgcccc
    25561 ttccattcac ggcgatagca ccacaataca gggctgtttg gcagcagatc ctggtaattg
    25621 ccccatgtca gaacggcttc ctgcttacgc agtgcgatta acttttggta ggtgtaaaac
    25681 accgaggaat catcggccaa cgcggcttct acgttgattt gttgatagtt atcgcccagg
    25741 ccaatccacg gttcgccagc cgtaaacccg gcattatcgc cgttgctcca ttgcatgggc
    25801 gtgcggctgt tgtcacggga tttactggcg aggattgcca ataactcgtc ggcatcacgc
    25861 ccatcgttgc gcagctcggc aaacatattg aggctctcta cgtcgcgata gtcagtaatg
    25921 cgcgtgaaat gcgggttagt catgccaatc tcttcgccct ggtagatata cggcgttccc
    25981 tgcatgccat gcagcaccat cgccagcatt tttgccgcag gcacgcggta ttcaccttca
    26041 tcaccaaagc gagaaacaat acgcggctga tcgtggttac accagaacaa ggcattccat
    26101 gccacgttgt gcattccttg ttgccagtgg cggaacaatg ttttcaacgc cacaaagtca
    26161 ggtttagcca gcgtccattt ttcaccaccg ggataatcga ccttcaggtg atgaaaatta
    26221 aaggtcatcg ataattcact gcctgtcagt gccgcgtatc gctggcaatg ctcaagactg
    26281 gtggaggaca tttcacctac ggtcattaac ccgcgtggcg taaacacatc gcggttcatc
    26341 tcgtgcaaaa actcgtgtgc tcgtggcccg tcggtgtaga agcgacgccc gtcgccgtcc
    26401 aggtcttcag ggaaacgcgg gtctttggag atcagattca ccacatccag gcgcaacccg
    26461 tcgaccccac gatcggccca gaactcacag acttttttca gctctgcgcg taccgctgga
    26521 ttctcccagt tgagatccgc ctgttctggt gcaaagagat gcaaatagta ctgttcgctt
    26581 tccgcatgcc agcgccacgc actaccgcca aattttgaac gccagttgtt cggtggcgtt
    26641 tctggttcgc catcgcgcca gatataaaac tggcggtaag ggctttcttt gttcagcgcc
    26701 tcgcgaaacc aggcatgttg ggtagaggta tggttaaaca ccatatcgag aatgatacga
    26761 atcccgcgcg attttgcctg cgtcaccagt tcgtcaaaat cgtccagcgt gccgtaggtg
    26821 ggatcaatcg ccgtatagtt cgctacgtcg taaccgttat cgacctgggg agagacataa
    26881 aagggggtta gccagatggc atcaacgccc agtttatgca gatagtccag gcgttggata
    26941 acgccacgta aatcgccggt gccgctaccc gtggtgtcct gaaaactctt tggataaatc
    27001 tggtagataa cgccgttttg ccaccagtgg ggaagattag tcattacgtt attcctgcaa
    27061 atgcgagggg gcgcaattgc gccccgaaga aaattaaaca atgtccagcg tgcccaggcg
    27121 gtatttccgc tgatagataa acgaggtgag tacaatcggg atgatgatgg cgatagccat
    27181 tgccagcgca aacacctgcc agtagctcgg ttgaatcgag agaattcccg gcaggccgcc
    27241 tacgccgatg ccattcgcca taacgccgtt caggccgcat agcaatcctg ccagaccaga
    27301 accaatcatc gcgcacagca tcgggaagcg atatttcagg ttgatgccgt acattgcagg
    27361 ctcagtgacc ccaagccagg cggagatcgc cgcaggcacg gagatctcgc gttcattgtg
    27421 tttgcggctg gaaatgataa tgcctatcac ggcggagccc tgagcgatat tcgacagcgc
    27481 aatcagcggc cacactggcg taccacccat gctttgaatc atctgcaaat caatagcaag
    27541 cgtggtctgg tgtaccccgg tgatcaccag cggggcgtac aggaagccaa acaatgccgc
    27601 gccaatcgga gcaaagctgc cggtcatcag gtgacgtacc gcaaaggcaa cgccatcgcc
    27661 aatcatgcga ccaaatggac caatcagcgc atgggcgagg aacaccgcga ggatcagcga
    27721 acagacgggc accaccacca gatagaggta atccggcacg atgcgtttaa ggcgagtttc
    27781 aataacgccc agcgccagcc cggctaacag tgccgggatc acctgcgcct gatagcccac
    27841 tttggcgatg ctgaacatgc caaagtccca cacttccggc agctgctgcc cgagcagata
    27901 agcgttcatc agctgggggg aaaccagtgt cacgccaagc acgataccaa ggatcggcgt
    27961 gccgcccatt tttttcaccg ctgaccagca aatcccgacc ggcaggtaga agaagatcgc
    28021 ttcaccgatc aaccacagaa aatcgtagat cgtttgcagg gaagggtaca tttgcgccag
    28081 cgtttgaccg ttgctcatgg gcaaatcgcc gatcacattg cgaaaaccga ggatcaaacc
    28141 gccgctaatc aacgcgggca gcaacgggaa gaagatctcc gcgaaatgag agatcaactg
    28201 ctcatgccat ttcatattct gccgggcggc tttttttacc tgctctttat caacttgcgc
    28261 ctgtccggtt gacgctatca gtgcttgata gtaatcaccc acgttggtgc caatcaccac
    28321 ctgaaattgc ccggcattgg tgaaacagcc tttcaccatg gggagttgct caatttcttt
    28381 cggtctggca ttggccggtt ggttgaggac aaagcgcagg cgagtaatac agtggctcac
    28441 cgtcgcaata ttgccgcgcc cgccgaccag ttcaatcaac cgatcgatat ccgtttggtt
    28501 tattttgctc atcataaagc cccatggcag atgacatttt tggttggctg cagaatatat
    28561 tgcgcggaaa aatttaaaaa cgggaacgtt cccgaaacgc agcgaagatc acaatttatc
    28621 gttcaggaaa cgatcaggac agggtggcgg ggatgatgat ttgttgcggt tcgctgcgcc
    28681 cggttacctg tgcgatcaac tggcaggccg cctggcgtcc agcttcggcg taaccgggat
    28741 ctacggtgac gatctccgga tggaggaatt tcattaacgg cgtattaccg acgctcgcca
    28801 gttgcaaggt gtcgatgcgt tgctcttgca ggtatttact tgcgccaagt gccagcgtgt
    28861 cggttgcgca cagtaaggcg gtagtttcag gcgtaatcac ttttgctacg ttctcatagc
    28921 cttgcttcat agcaaggcct ggcagggcgg caacgggatg cagtttatgc gctttgcaga
    28981 acgccaggta ggcttcgtga cgtcgcttac cggttgtcac gtcactgtgc ggcacgccga
    29041 gataactgat attacgatgc ccctggtcat acagccgttg catcaggatc ttgattgccc
    29101 cttcgtcgtc ataacagacc gaagcaaagc cttttgcgtc acgcgccagc agaaccagcg
    29161 atgactgcca gtgggctaac atttcttctg ttatgccggt aaaaccgaac agcactacgc
    29221 cgtcgatatt acgccgtttc agcaccccca aatgttcggc aactaattgc ggggaaaact
    29281 gactttccat catgattggg tcgtaacctt gttcatagaa cgctggcagc atggtttgaa
    29341 cggcgagatt ttctgacaac gaatccagac gggtaacaat gatggcgacc actttatcgc
    29401 tttgcccacg catagcgcgt gcagaacggg aaggggaaaa tccatgctga ttcatcactg
    29461 cttcaacacg ctcgcgggtg cgctggctca cgccgctttc gttattcagc acccgggaaa
    29521 ctgtagattt ccccacgccg cttaagcgcg cgatgtcttt gatggtcagc cgattttgca
    29581 tcctgttgtc ctgtaacgtg ttgtttaatt atttgagcct aactttaccc gtgcattcag
    29641 caatgggtaa agtctggttt atcgttggtt tagttgccag caggtattat atcgccatag
    29701 atgctacgaa tattattgga ttctccttat catttgcggc gcttttttca cttaccggag
    29761 gttatatgga acctgatccc acgcctctcc ctcgacggag attaaaactt ttccggtaag
    29821 cccgtctttt cacggcgtta ccggatgcgt aaggccgtga cgttttaacg tccctgctca
    29881 gctttattac cttcaggtaa ggcttcgcca cgcctgaaga catttctgta ctgtttcaga
    29941 cagtgcggag ggactcctta tgtttaaaga aatttttacc cggctcattc gccatttacc
    30001 ttcccgtctg gttcatcgtg atccattgcc tggcgcgcag cagacagtga atacggtggt
    30061 cccgccgtcc ttaagtgcgc attgcctgaa aatggcggtg atgcccgaag aagaattgtg
    30121 gaaaacgttc gacacccatc cggaagggtt aaatcaggcg gaagtggaat ctgcccgcga
    30181 acaacatggt gaaaataaat tacccgcaca acaaccgtcg ccgtggtggg tacatttatg
    30241 ggtctgctat cgcaacccct ttaatatttt actcaccatt ctcggcgcta tttcttacgc
    30301 cacggaagat ttatttgctg cgggcgttat cgcgctaatg gtcgctattt ctacgttgct
    30361 gaactttatt caggaagcac gttccactaa agcggcagat gccctgaaag cgatggtcag
    30421 caatactgcg acggtgctgc gcgtaattaa cgacaaaggc gaaaatggct ggctggagat
    30481 cccgatcgac cagctggtgc ccggcgatat tataaaactg gcggcgggag atatgatccc
    30541 ggcagattta cgtatcttgc aggcgcggga tctgttcgtc gctcaggcgt cgttaaccgg
    30601 tgagtctctg cccgtagaaa aagccgctac cactcgccag ccggagcaca gcaatccgct
    30661 ggagtgcgac acgctgtgtt ttatgggcac caccgtggtg agcggcacgg cacaagcaat
    30721 ggtgattgct acaggtgcca atacctggtt tggtcaactg gcggggcgtg ttagtgagca
    30781 ggaaagcgag ccgaatgcct ttcagcaagg gatcagccgc gtcagtatgc tgctgattcg
    30841 ctttatgctg gtgatggcac cggtggtgct gttaatcaat ggctacacca aaggcgactg
    30901 gtgggaagcg gcgctgtttg cgctttcggt cgcggtaggc ctaacgccgg aaatgttgcc
    30961 gatgattgtt acctcgacgc tggcgcgcgg cgcagtaaag ctgtcgaaac agaaagtgat
    31021 cgtcaaacat ctggatgcta ttcagaactt tggcgcaatg gatattctgt gcactgataa
    31081 aaccggcacc ctgacgcagg ataaaattgt gctggagaat cataccgata tctccggtaa
    31141 aaccagcgaa cgcgtgctgc atagcgcgtg gttgaacagt cattatcaga ccggacttaa
    31201 aaacctgctc gatacagcgg tgctcgaagg tacggatgaa gagtcagcgc gctcgctggc
    31261 cagtcgttgg caaaaaatcg atgagattcc gtttgatttc gagcgtcgcc ggatgtcggt
    31321 ggtagtggca gaaaataccg agcaccatca gctggtttgc aaaggcgcat tgcaggaaat
    31381 cctcaatgtg tgttcgcagg tgcgtcacaa tggcgagatt gtgccgctcg atgacatcat
    31441 gctgcgtaag attaagcggg ttactgatac gctgaatcgt caggggctgc gcgtggttgc
    31501 ggtggcgacg aaatacctgc cagcgcgtga aggagattac cagcgggcgg atgaatccga
    31561 cctgatcctt gaaggatata ttgcttttct tgatccgcca aaagagacaa ccgctccggc
    31621 actgaaggca ttaaaagcga gtggaattac cgtaaaaatc cttactggcg acagtgagtt
    31681 agttgcagcg aaagtgtgcc atgaagtggg gctggacgcg ggagaggtgg tcattggtag
    31741 tgatattgaa acgctatctg acgacgaact ggcaaatctt gcacagcgca ccacgctgtt
    31801 tgcccgcctg acgccgatgc ataaagaacg cattgtgacc ttactgaagc gcgaagggca
    31861 tgtggttggc tttatgggcg atggtattaa tgatgcgccc gccttacgcg cggcggatat
    31921 cggcatttct gtggacggcg cggtagatat tgcccgtgaa gcggctgata tcatcctgct
    31981 ggaaaaaagc ctgatggtgc tggaagaggg ggttattgag ggacgtcgca ctttcgccaa
    32041 catgctgaaa tacatcaaaa tgacggcgag ttctaacttc ggtaatgtgt tcagcgtgct
    32101 ggtggcgagt gctttcttgc ccttcctgcc gatgttgccg ttacacttgc ttattcagaa
    32161 cctgctgtac gatgtgtcac aggtggcgat cccgtttgat aatgtcgacg acgagcaaat
    32221 tcaaaagccg cagcgttgga atccggcgga tctggggcgc tttatgatct tcttcggacc
    32281 gatcagctcg atcttcgata ttctgacgtt ttgcctgatg tggtgggtat tccatgccaa
    32341 cacgccggaa acgcaaacgc tgttccagtc gggatggttt gtggtgggct tactgtcgca
    32401 aacgctgatt gtgcatatga tccgcacccg ccgtgtgccg tttattcaga gctgtgcatc
    32461 gtggccgtta atgatcatga ccgtgatcgt gatgattgtc ggtatcgcat tgccgttttc
    32521 accgctggcc agttatctgc agctgcaggc gctgccgtta agctatttcc cgtggctggt
    32581 tgcgattctg gcagggtata tgacattaac ccagttggtg aaagggttct atagccgtcg
    32641 ttacggctgg caataaagaa tcaactgggc atggtgaccc ggattcagat tgcagacaaa
    32701 gtgagttttg ttcatgccgg atgcggcgta aatgccttat ccggccaaca aattcaataa
    32761 attgcaggaa tcacgtaggc ctgataagcg tagcgcatca ggcgattttg cgtttgtcat
    32821 cagacttaat ccgggcatga tagcccggat ttccatcaag attagcgacg aacagcgatc
    32881 gcttcgatct caatcttcac gtctttcggc agacgggcta cttcaacgca agaacgtgcc
    32941 gggaaggtgg cgttgtgttc ggtgaagaag gcttcgtaag tggcgtttac ggttgcgaag
    33001 tcgttcagat cttttacaaa cacggtagtt ttaacgatgt cgcccacttt caggccagcg
    33061 gcttcgacga tcgcttttac gttatccagc gactgacgtg cctgtgcagc gacgtctgcc
    33121 ggtacttcgc ccgttttcgg atttaccggg atctgaccgg aggtgatgat catattgccc
    33181 agatcaacgc cctgtacgta aggaccgata gctgccggtg cattttccgt cgcgatagtt
    33241 ttgctcatga tttctccttt attacagcgg taaaaagtct ggctcattat agggagccag
    33301 acttttatta ccaaccgcaa ttaattggcc agcaccacat tatgggaaaa ctctttttca
    33361 cagtatttgc atttgagcgc gatgtcattg gcgcgttttc gcacggcaaa gctggaagaa
    33421 accggttcgg catggctgat acagttgctg ttcgggcaga ccagcacatt gtcgatgcgc
    33481 tccggcagac ttgggcgcga tttacccacc acttcatagt tgtcgatacg gttaaccgtg
    33541 gcttgcggcg catacaatgc cagttgatct acctggtctt cactcaaaaa ggtattttcg
    33601 attttgatca gatctttgcg gcccatctcg ccagaaggca ggttcagacc gatggtgatg
    33661 cggtgatccg tttcggtcag cttgaacaga ctcaacagct taaaaccgat ctgggcgggg
    33721 atatggtcaa ttaccgtgcc gcgtttaata gcttcaacct gcaatttatt atcgtgtgtc
    33781 atctctattt ccccttacag taccagatcg cgattcagaa ccagtgccag taacgcctgg
    33841 cgagcgaaaa tcccgttgcc tgcctgctgg aagtaccagg cgtgtggcgt tttatcaaca
    33901 tccgtcgcaa tctcatcaac acgcggcagc ggatgaagta ctttcatatt ggctttggcg
    33961 ttgtggagat cgctggcgcg aagaacaaac tgcgctttca cgttggcgta ctcggacggg
    34021 tccagacgct ctttttgcac gcgggtcatg tacaggatgt caacttccgc catcacttct
    34081 tcaatagagc tgtgcagact ccatgcgatc cctttttcat caagcatatc cagaatgtat
    34141 tgcggcattg ccagcgcgtc cggcgcgatg aagtaaaaac ggttgccgtc gaacttcgct
    34201 aacgcctgag tcagggagtg aacggtgcgg ccatatttca ggtcaccaac cattgcgacg
    34261 tggagattgt ccagacgccc ctgggtttcc tgaatggtga ataagtccag caaggtttgc
    34321 gtcggatgtt ggttggagcc atcaccggca ttcagtaccg gtacattgcc ggaaaactcg
    34381 gtggccaggc gcgccgcacc ttcctgcgga tgacgcatca ctatcgcatc gacgtaagtg
    34441 ctgataaccg aaatggtatc ggccagcgtt tcgccctttt tacccagtga tgtattggcg
    34501 ctgtcggaga agcccaccac gctggcaccc aggcggtgca tggaagtttc gaaagagagg
    34561 cgggtacggg tagaggcttc gaagaaacag ctggcaatga ctttgtgctt caacagctct
    34621 ggttgcgggt ttgctttcag tttcgccgct gtcgccagca ccagattaag gtcatcgcga
    34681 ctaaggtcgt ttatggaaat gatatgtttc tgatatagcg gattagccat cttttatctc
    34741 ctgacgcctg ggcaaaaaaa agcccctcga ttgaggggct gggaatgggt gatcaacggg
    34801 aagaaaaacg gcaggccagc gtcttttttc agacgcggta agacaaaatg tcgaacacac
    34861 tgaaccatac atcctcccgg caaattgtcc ggcattatac tcatcgtcag aagcggtgca
    34921 agcatttgat gcaatatttt gtcagcgcaa acggtttatt tgaattataa gtcaaaatat
    34981 atgcattttt atttactgtg attctggagg ggaactgtcg ttcggtatca ataattgcag
    35041 acatttcctg cttttttctt tttcactttc acgcaatcag atatgcattt tattccattc
    35101 atccgactta tagggcggag tgtgaaaacg aacggctaac actattgctt acagctcagg
    35161 gatgcgcgct atcactttaa tttcaaaatc aaagcctgcc agccatgtaa cgcccaccgc
    35221 cgtccaggtt ggataaggtg gcgcgctaaa tatttcattt ttcaccgtca tgatgtcttc
    35281 aaattgtttt tctggatcgg tatgaaagct cgtaacatca atgatatcgt caaaagtgca
    35341 tcccgcagct gccagggtcg catgcaaatt atcaaatgcc agtctgactt gttgctgaaa
    35401 atcgggttct ggtgttccgt cctctcgact tcctacttgc ccggaaacaa acagcaaatc
    35461 gccggaacga atagccgcag aataacgatg ttctgcatac agtgaatgtc ggccagcagg
    35521 gaaaacagcg gttctttcta ccatttggtt atcctcaaga tttacgacat gaacagaaga
    35581 tttatattta ccaggagccg cttttagcgg acgacgtgag taaacaaaac ccagacatca
    35641 tgggaaatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg ccatcaatgg
    35701 tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg accataccag
    35761 cgacctcttc cggttgccca tgtcttttga tagccataaa actatgcaac atatcgcgca
    35821 ttggcccgtt ggcgggatta gcgtcggtat caattggccc tggctggacg acgttgatgg
    35881 tgatcccacg cggtccaaaa tcacgggcca gcccgcgcgc catgccttgt agggcagatt
    35941 tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc acggagccga
    36001 tgattaagat gcgcccgcct tcgggcatct gccgggcggc ttcaacagag gcatggtaag
    36061 gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat tccagggcat
    36121 cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg ctcttacgaa
    36181 cgacatcaat gacagcgtct ctgtcagcac tatctgtgaa tactgctgtc gctccagtct
    36241 cttgtgccag gtgttcagcg gcatctttcg accccgcata ggtgaatcgt acattggccc
    36301 catcggtgac gaaacgacgt acgatagcgg caccgatacc acgactgcca ccgaggatga
    36361 gaactgtctt acctgtaaaa gcgcccataa ggactccttg atttattatg taacatgcat
    36421 tacaaaactg ttttaacttt ctgtcaataa gttttgtaat ggtcactaaa aaacaatctc
    36481 gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg gcaaaagtgc
    36541 tttttcacca aaaaggtttc gatgctgtca gtgttgctga agttactgat tatcttggta
    36601 ttaacccccc gagcctctac gcggcttttg gcaataaggc tgggttattt agccgtgtac
    36661 tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt gatgatcgtc
    36721 cggtagggga gtgcctggct gaggtattaa aagaagcggc gcgcagatat agccaaaacg
    36781 gcggctgcgc tggctgtatg gttcttgaag gcattcatag tcatgatcca caagcgcgtg
    36841 atattgccgt tcaatattat cacgccgcag aaacggccat ttatgactat atcgccaggc
    36901 ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg tcagggcttt
    36961 ctgcgaaggc acgagagggg cactccctcg aacaactctg tgcaacagct gcactggcgg
    37021 ggggagcgat aaaaactatt ctcaaggagt gatgcgggcc ttgacccgaa aggcgggaac
    37081 gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctag cggaattgat
    37141 catcttttcg tataacgata gaaatgaaac gttgttttaa ttaaggagtg gaaaagatga
    37201 tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc caggcgattg
    37261 agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcacgat atcgaaggca
    37321 atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gcacgaagct cgccgtgcgg
    37381 agtaccatgc ccgctatctc gacattcaga ttgtgttaaa aggtcaggaa ggcatgacct
    37441 tcagcacgca acctgcaggc accccggata ccgactggtt agctgataaa gacatcgcat
    37501 ttttgccgga aggcgttgat gagaaaacag ttatcctaaa tgaaggtgat tttgttgtgt
    37561 tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcaccagcc caggttcgca
    37621 aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac tccaggttta
    37681 cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat ttgtaatgct
    37741 tagacgttct tattaactca aggagttcgt catgagcaaa atatcagatt tgaattattc
    37801 tcaacacatt acatcagccg ataattttaa accaaaaaat gaagtcttaa atacctggtg
    37861 tgtgggaatg aatgattttg cccgtattgc cggggggcag gatagcagaa gaaatattct
    37921 atctcctgga gcatttttgg cgtttttggc aaaaatattt accctgggtt atgtggattt
    37981 tagcaaacgc tccaacgaag cgggtagaaa tatgatggct catattgagt cctcatctta
    38041 tatcaaaaat aatgatggca gtgaaataat gaagtttgtt atgaataatc ctgaaggtga
    38101 gcgagcggat tcatctaagg tgattataga aatttcactt tccactacta ctatggggac
    38161 tcgtcaagga catacagcca ttatatttcc acaatctgat tttttgagtt ttcgttatga
    38221 agggaagtct tttgaaagaa aagatgatag ttcattacac ctgattacta acaaggttct
    38281 ggcgtgttac caacgcgaag ctaacaagga aatagcgcgt ctattaaata atcatcagaa
    38341 gtta
//
LOCUS       DAZFFG010000030        38079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFFG010000030 DAZFFG010000000
VERSION     DAZFFG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.030"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..521)
                     /locus_tag="ACHJ7J_001934"
     CDS             complement(<1..521)
                     /locus_tag="ACHJ7J_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5000171.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHL"
     gene            999..1787
                     /gene="fabI"
                     /locus_tag="ACHJ7J_001935"
     CDS             999..1787
                     /gene="fabI"
                     /locus_tag="ACHJ7J_001935"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO5000172.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSNIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1931..3058
                     /gene="yciW"
                     /locus_tag="ACHJ7J_001936"
     CDS             1931..3058
                     /gene="yciW"
                     /locus_tag="ACHJ7J_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO5000173.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVDLFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLEKLLNSTRVNTQPNNQLAALLCARIN
                     GSPACFATCMDSSNEYKKISTLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            3126..5060
                     /gene="rnb"
                     /locus_tag="ACHJ7J_001937"
     CDS             3126..5060
                     /gene="rnb"
                     /locus_tag="ACHJ7J_001937"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO5000174.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLFREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDEMVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(5138..5242)
                     /locus_tag="ACHJ7J_001938"
     CDS             complement(5138..5242)
                     /locus_tag="ACHJ7J_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000175.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            5296..7281
                     /gene="pdeR"
                     /locus_tag="ACHJ7J_001939"
     CDS             5296..7281
                     /gene="pdeR"
                     /locus_tag="ACHJ7J_001939"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5000176.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(7263..7391)
                     /gene="ymiD"
                     /locus_tag="ACHJ7J_001940"
     CDS             complement(7263..7391)
                     /gene="ymiD"
                     /locus_tag="ACHJ7J_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO5000177.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            7429..7602
                     /gene="yciZ"
                     /locus_tag="ACHJ7J_001941"
     CDS             7429..7602
                     /gene="yciZ"
                     /locus_tag="ACHJ7J_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO5000178.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            7691..8440
                     /gene="yciT"
                     /locus_tag="ACHJ7J_001942"
     CDS             7691..8440
                     /gene="yciT"
                     /locus_tag="ACHJ7J_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO5000179.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDASCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            8709..8927
                     /gene="osmB"
                     /locus_tag="ACHJ7J_001943"
     CDS             8709..8927
                     /gene="osmB"
                     /locus_tag="ACHJ7J_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO5000180.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9053..9379)
                     /gene="yciH"
                     /locus_tag="ACHJ7J_001944"
     CDS             complement(9053..9379)
                     /gene="yciH"
                     /locus_tag="ACHJ7J_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO5000181.1"
                     /translation="MSDSNSRLVYSTDTGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9379..10116)
                     /gene="pyrF"
                     /locus_tag="ACHJ7J_001945"
     CDS             complement(9379..10116)
                     /gene="pyrF"
                     /locus_tag="ACHJ7J_001945"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO5000182.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(10309..11478)
                     /gene="lapB"
                     /locus_tag="ACHJ7J_001946"
     CDS             complement(10309..11478)
                     /gene="lapB"
                     /locus_tag="ACHJ7J_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO5000183.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEVNRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(11485..11793)
                     /gene="lapA"
                     /locus_tag="ACHJ7J_001947"
     CDS             complement(11485..11793)
                     /gene="lapA"
                     /locus_tag="ACHJ7J_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO5000184.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(11942..12706)
                     /gene="pgpB"
                     /locus_tag="ACHJ7J_001948"
     CDS             complement(11942..12706)
                     /gene="pgpB"
                     /locus_tag="ACHJ7J_001948"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO5000185.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            12876..13466
                     /gene="ribA"
                     /locus_tag="ACHJ7J_001949"
     CDS             12876..13466
                     /gene="ribA"
                     /locus_tag="ACHJ7J_001949"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO5000186.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(13530..16205)
                     /gene="acnA"
                     /locus_tag="ACHJ7J_001950"
     CDS             complement(13530..16205)
                     /gene="acnA"
                     /locus_tag="ACHJ7J_001950"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO5000187.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(16369..16464)
                     /gene="ymiC"
                     /locus_tag="ACHJ7J_001951"
     CDS             complement(16369..16464)
                     /gene="ymiC"
                     /locus_tag="ACHJ7J_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO5000188.1"
                     /translation="MINTNMKYWSWMGAFSLSILFWAELLWIITH"
     gene            complement(16578..16745)
                     /gene="yciX"
                     /locus_tag="ACHJ7J_001952"
     CDS             complement(16578..16745)
                     /gene="yciX"
                     /locus_tag="ACHJ7J_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO5000189.1"
                     /translation="MVGQEQLESSPLCQDSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(16748..16888)
                     /gene="ymiA"
                     /locus_tag="ACHJ7J_001953"
     CDS             complement(16748..16888)
                     /gene="ymiA"
                     /locus_tag="ACHJ7J_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO5000190.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(17207..18181)
                     /gene="cysB"
                     /locus_tag="ACHJ7J_001954"
     CDS             complement(17207..18181)
                     /gene="cysB"
                     /locus_tag="ACHJ7J_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO5000191.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(18391..20988)
                     /gene="topA"
                     /locus_tag="ACHJ7J_001955"
     CDS             complement(18391..20988)
                     /gene="topA"
                     /locus_tag="ACHJ7J_001955"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO5000192.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21368..21619
                     /gene="yciN"
                     /locus_tag="ACHJ7J_001956"
     CDS             21368..21619
                     /gene="yciN"
                     /locus_tag="ACHJ7J_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO5000193.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(21655..22704)
                     /gene="sohB"
                     /locus_tag="ACHJ7J_001957"
     CDS             complement(21655..22704)
                     /gene="sohB"
                     /locus_tag="ACHJ7J_001957"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO5000194.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            22924..23682
                     /gene="yciK"
                     /locus_tag="ACHJ7J_001958"
     CDS             22924..23682
                     /gene="yciK"
                     /locus_tag="ACHJ7J_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO5000195.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            23679..24269
                     /gene="cobO"
                     /locus_tag="ACHJ7J_001959"
     CDS             23679..24269
                     /gene="cobO"
                     /locus_tag="ACHJ7J_001959"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5000196.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(24309..25181)
                     /gene="rluB"
                     /locus_tag="ACHJ7J_001960"
     CDS             complement(24309..25181)
                     /gene="rluB"
                     /locus_tag="ACHJ7J_001960"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO5000197.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(25394..27289)
                     /gene="yciQ"
                     /locus_tag="ACHJ7J_001961"
     CDS             complement(25394..27289)
                     /gene="yciQ"
                     /locus_tag="ACHJ7J_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO5000198.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPNGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQTLIRV
                     NEGVTRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALPGWAVSAN
                     LGREWVARITPSLTAAVSAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGAAGGGAG
                     GGGGGGW"
     gene            complement(27317..27937)
                     /gene="yciO"
                     /locus_tag="ACHJ7J_001962"
     CDS             complement(27317..27937)
                     /gene="yciO"
                     /locus_tag="ACHJ7J_001962"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO5000199.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(27934..28815)
                     /gene="rnm"
                     /locus_tag="ACHJ7J_001963"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(27934..28815)
                     /gene="rnm"
                     /locus_tag="ACHJ7J_001963"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO5000200.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            28953..28997
                     /gene="trpL"
                     /locus_tag="ACHJ7J_001964"
     CDS             28953..28997
                     /gene="trpL"
                     /locus_tag="ACHJ7J_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO5000201.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    28974..29067
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            29089..30651
                     /gene="trpE"
                     /locus_tag="ACHJ7J_001965"
     CDS             29089..30651
                     /gene="trpE"
                     /locus_tag="ACHJ7J_001965"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO5000202.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDTALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGALDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            30651..32246
                     /gene="trpD"
                     /locus_tag="ACHJ7J_001966"
     CDS             30651..32246
                     /gene="trpD"
                     /locus_tag="ACHJ7J_001966"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO5000203.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPEQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            32247..33608
                     /gene="trpCF"
                     /locus_tag="ACHJ7J_001967"
     CDS             32247..33608
                     /gene="trpCF"
                     /locus_tag="ACHJ7J_001967"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO5000204.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNIEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            33620..34813
                     /gene="trpB"
                     /locus_tag="ACHJ7J_001968"
     CDS             33620..34813
                     /gene="trpB"
                     /locus_tag="ACHJ7J_001968"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO5000205.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTKTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            34813..35619
                     /gene="trpA"
                     /locus_tag="ACHJ7J_001969"
     CDS             34813..35619
                     /gene="trpA"
                     /locus_tag="ACHJ7J_001969"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO5000206.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            36000..36179
                     /gene="yciG"
                     /locus_tag="ACHJ7J_001970"
     CDS             36000..36179
                     /gene="yciG"
                     /locus_tag="ACHJ7J_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5000207.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQNGGNKSGKS"
     gene            36265..36765
                     /gene="yciF"
                     /locus_tag="ACHJ7J_001971"
     CDS             36265..36765
                     /gene="yciF"
                     /locus_tag="ACHJ7J_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5000208.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            36811..37317
                     /gene="yciE"
                     /locus_tag="ACHJ7J_001972"
     CDS             36811..37317
                     /gene="yciE"
                     /locus_tag="ACHJ7J_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5000209.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(37377..38015)
                     /gene="ompW"
                     /locus_tag="ACHJ7J_001973"
     CDS             complement(37377..38015)
                     /gene="ompW"
                     /locus_tag="ACHJ7J_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO5000210.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT         9592 a         9833 c         9192 g         9462 t
ORIGIN      
        1 agatgttgtg gatcgctttt ttgcggtgtt aaacgacgat aaaccgtacc ttccatcatg
       61 gtagcaaaca cttcaacgca gcaacgaatg tgctcgtcgc taagatgggg atgcattctt
      121 ttcatatgtg cacaaccgtt agcgaacatt ctggcgtcag cttcttcaag cattgccata
      181 acccgcgggt tgcgtgtggc ttctgcagcc acttccagca tcagcgcgtc gtcgtcctca
      241 tttaaggatt tacgcaaggc aaggacttcc gggaagtgat cggtacgcgc gtcaatgtcc
      301 atttgggcaa tacgaaagtc gatgatacgg cgaaccatct cttcaataat ggcgtctttg
      361 ttggcgaagt aacgatatat ctgacccacg ctaagctgag cttcgctggc aatttgcgac
      421 atactggcgg catgaaaccc gcagcgacga aaacaccgac gggcggcgtt aatgatttca
      481 tcctgacgct gtttgtggcg tatctccagc ttgctggtca tttctgtctc ctggcctatc
      541 acttggtgag aatgatcgtt ctcatttttg ctgattatac gtttctccaa agtaatggaa
      601 agtattttta tcttattcat ggtgcatatt ctgataatga agagattgtg gagattctgt
      661 gaagatagtt gacaatatta agttggggtc aattcatcag aaaggctgct acttgttgtc
      721 gccagttctg atttcatcat caaacttatc ggagctaata cgaatagttt catagtcaga
      781 ttctataaga tatttcccca acctacccct tgcaggcgtc aagagtagat aacgcaaaat
      841 gctctgtacc gcagcttctc tccggtacag aaagcgcaac tatagccacc cacagccagg
      901 ttgattataa taaccgttta tctgttcgta ctgtttacta aaacgacgaa tcgcctgatt
      961 ttcaggcaca acaagcatca acaataagga ttaaagctat gggttttctt tccggtaagc
     1021 gcattctggt aaccggtgtt gccagcaaac tgtccatcgc ctacggtatc gctcaggcga
     1081 tgcaccgcga aggagctgaa ctggcattca cctaccagaa cgacaaactg aaaggccgcg
     1141 tagaagaatt tgccgctcaa ttgggttcca atatcgttct gcaatgcgat gttgccgaag
     1201 atgccagcat cgacaccatg ttcgctgaac tggggaaagt ttggccgaaa tttgacggtt
     1261 tcgtacactc tattggtttt gcacctggcg atcagctgga tggtgactat gttaacgccg
     1321 ttactcgtga aggcttcaaa attgcccacg acatcagctc ctacagcttc gttgcaatgg
     1381 caaaagcttg ccgctccatg ctgaatccgg gttctgccct gctgaccctt tcctaccttg
     1441 gcgctgagcg cgctatcccg aactacaacg ttatgggtct ggcaaaagcg tctctggaag
     1501 cgaacgtgcg ctatatggcg aacgcgatgg gtccggaagg tgtgcgtgtt aacgccatct
     1561 ctgctggtcc gatccgtact ctggcggcct ccggtatcaa agacttccgc aaaatgctgg
     1621 ctcattgcga agccgttacc ccgattcgcc gtaccgttac tattgaagat gtgggtaact
     1681 ctgcggcatt cctgtgctcc gatctctctg ccggtatctc cggtgaagtg gtccacgttg
     1741 acggcggttt cagcattgct gcaatgaacg aactcgaact gaaataatcg ttctgttggt
     1801 aaagatgggc ggcattctgc cgcccgttat ctctgttata cctttctgat atttgttatc
     1861 gccgatccgt ctttctcccc ttcccgcctt gcgtcaggat aacgatttcc tttacgacca
     1921 aggagcgccc atggaacaac gccacatcac cggcaaaagc cactggtatc atgaaacgca
     1981 atccagtact acggagtatg acgttctgcc tctggtcccg gaagccgcaa aggtcagcga
     2041 tccctttcta ctcgacgtga tccttgaaaa agaaacgctg gcccccttcc tttcatggct
     2101 ggaccctgcg cgtgttcttg cagtggattt gttccctgac cagcttaccg tgacccgttc
     2161 acagaccttc accgcttatg aacgcttgtc gacggccctg acggttgctc aggtttgcgg
     2221 cgtccagcgg ttatgtaact actattcggc gcgacttacg ccgctccccg ggcctgattc
     2281 caccagggaa agtaatcatc ggttggcaca aatcacgcaa tatgcccgcc aactggctag
     2341 ctcgccttct attatcgaca accgatcgcg ccagcatctg aatgacgtcg gtcttactgc
     2401 ctgggactgt gtgatcatta accaaatcat tggttttatt ggctttcagg cgcggacaat
     2461 tgcgacattt caggcttatc tcgggcatcc ggtacgctgg ttacccgggc tggagataca
     2521 aaactacgcc gacgcgtcac tgtttgctga tgaatcatta cgctggcgaa gcagctatga
     2581 ggtggaaaaa ctacctgaag agcacacaaa aagttcaact gcagaacttt gccaactggc
     2641 cgaaatactc tctctccacc ctatttcact ttcccttctc gaaaagttat taaacagcac
     2701 acgggtcaat acacagccga ataatcagct tgcggcgttg ttatgcgcgc ggataaatgg
     2761 cagtcctgct tgttttgcca cctgtatgga ttcatcaaat gaatataaaa aaatcagcac
     2821 ccttctgcgc aagggcgaaa atgaaattaa ccaatgggct gaccgtcatt ctgttgagcg
     2881 cgctaccgtt caggcgatac aatggctgac ccgagcaccc gatcgcttta gcgccgccca
     2941 gttcagccct ttactcgaac acgaaaaatc atcaacgcag attattaatc tgctggtatg
     3001 gagcgggctg tgtggctgga taaatcgctt aaaaatcgcg ttgggtgaga catattaacc
     3061 ttgccgcgtc agacagattc gcgtaaaact gtcagccgct ctaatggcca ccaaaataga
     3121 caattatgtt tcaggacaac ccgctgctag cgcagcttaa acagcaactg cattcccaga
     3181 cgccacgcgc tgaaggggtg gtaaaagcca cagaaaaagg ttttggcttc ctggaagtcg
     3241 acgcgcaaaa aagttatttc attccgccgc cgcagatgaa aaaagtcatg catggcgacc
     3301 gaattatcgc ggtgatccac agtgaaaaag aacgtgaatc cgcagagcca gaagaactgg
     3361 ttgaaccgtt tctgactcgt ttcgtgggta aagttcaggg caaaaatgac cgtctggcca
     3421 tcgttcctga tcatccactc ttaaaagacg ccattccttg ccgcgcagcc cgtggtctga
     3481 accacgagtt taaagaaggc gactgggcag ttgccgaaat gcgccgtcat ccgctgaaag
     3541 gcgatcgttc tttctatgct gaactgacac aatacatcac ttttggtgac gaccactttg
     3601 taccgtggtg ggttacactt gcgcgccata atctggaaaa agaagcacca gacggcgtcg
     3661 ctaccgaaat gctcgatgaa ggtctgtttc gtgaagatct gaccgcgctg gattttgtca
     3721 ccatcgacag tgccagcaca gaagatatgg atgacgccct tttcgctaag gcgttgccgg
     3781 atgacaaact tcagctgatt gtggcgattg ccgatccaac cgcgtggatt gctgaaggca
     3841 gcaagctgga caaagccgcg aaaattcgcg cattcaccaa ctatctgcct ggcttcaaca
     3901 tccctatgct gcctcgcgag ctttctgacg atctctgctc actgcgcgcc aatgaagtcc
     3961 gcccggtact ggcatgccgc atgacgctct ccgctgatgg caccattgaa gataatatcg
     4021 aattctttgc cgccaccatc gaatccaaag cgaagctggt gtatgaccag gtttctgact
     4081 ggctggagaa taccggtgac tggcagcctg aaagtgaagc aattgccgaa caagtccgtt
     4141 tgctagcgca aatttgccaa cgacgcggcg agtggcgtca taaccacgca ctggtgttta
     4201 aagatcgccc ggattaccgc tttattctcg gtgaaaaagg tgaagtgctg gatatcgtcg
     4261 ccgagcctcg tcgcattgcc aaccgtatcg tcgaagaagc gatgattgcc gctaatattt
     4321 gtgcggcccg cgtactgcgc gataagctcg gttttggtat ctataacgtg catatgggct
     4381 ttgatccggc gaatgccgac gcgctggcag cgttgctgaa aacgcacggt ctgcatgtcg
     4441 atgccgaaga agtgctcacg ctggacggtt tctgcaaact gcgtcgtgaa ctggatgcgc
     4501 aaccaactgg tttcctcgac agccgcattc gtcgcttcca gtcatttgct gaaattagca
     4561 ctgaacccgg tcctcacttt ggcctcggtc tggaggcata cgccacctgg acttcgccga
     4621 tccgtaaata tggcgacatg atcaaccacc gtctgctgaa agcggttatc aaaggcgaaa
     4681 ctgcgacgcg tccacaggat gaaatcactg tgcaaatggc cgagcgtcgc cgtctcaacc
     4741 ggatggcaga acgtgatgtt ggtgactggt tatacgcacg cttcctgaaa gacaaagccg
     4801 ggaccgacac ccgtttcgca gcggaaattg tcgatatcag ccgtggcggc atgcgtgttc
     4861 gtttggttga taacggcgct atcgccttta ttcctgcacc tttcttacac gctgtgcgcg
     4921 atgaaatggt ttgcagccag gaaaacggca ccgtacaaat taaaggtgaa acggtttata
     4981 aagtaactga cgttattgac gtcaccattg ccgaagtccg catggaaacc cgcagcatta
     5041 ttgcgcgccc ggtcgcgtaa tctcctttca cggcccattc cttatgaatg ggccgtttat
     5101 ttccccgctc taccttcatc atatcccggc agatttttta actgactttc gtttgaaaac
     5161 tggcgttttt ccaaaactgg attacaaata aatatataaa aaaaacaatg cgtttagccg
     5221 ccgttaattt catgtaagtc atgttgttcc ttttatttaa ccgcggactc cgcagttaac
     5281 cggaggatat gcatcatgaa aaccgttagg gagtccacaa cgttgtacaa ctttctcgga
     5341 tcgcacaatc catactggcg gttgacggaa agcagcgatg ttttgcgctt ttctaccacc
     5401 gaaaccacag aacctgatcg tactttgcag ttatctgccg aacaggctgc tcgcatcagg
     5461 gaaatgacgg tcatcacctc cagcctgatg atgagtctga ccgtcgatga gagcgatctt
     5521 tctgtgcatc tggtaggacg aaaaatcaat aaacgggaat gggctggcaa cgcgtctgca
     5581 tggcatgaca caccggcagt tgctcgtgat ttatcacacg ggctttcctt tgctgagcag
     5641 gtagtttctg aagcacattc cgcaatagtc attctcgaca gccgggggaa tatccagcgc
     5701 ttcaatcggt tatgtgaaga ttacacaggg ttgaaagaac acgacgtcat tgggcaaagc
     5761 gtgtttaaac tgtttatgag ccgtcgtgaa gctgcggcat ccaggcgcaa taaccgtgta
     5821 ttttttcgaa gcggcaatgc atatgaagtc gaactgtgga taccaacacg taaaggccag
     5881 cggctgtttc tgtttcgcaa taaatttgtc cacagcggta gtggcaaaaa cgagattttt
     5941 ttaatctgtt ccggcaccga cattaccgaa gagcgccgcg ctcaggagcg actgcgcatt
     6001 ctggcaaata ccgacagtat caccggactg ccgaatcgta acgcaatgca ggagttaatc
     6061 gatcacgcta ttaatcatgc agataataat aaagttgggg tcgtgtatct tgatttggat
     6121 aattttaaaa aggtcaacga cgcctatggg catttgtttg gtgaccagtt attacgcgac
     6181 gtgtcattgg ctattttaag ctgtctcgaa catgaccagg tgttggcgcg tccaggtggc
     6241 gatgagtttc tggtactggc atccaacacc tcacaaagcg cgctggaagc aatggcatca
     6301 cgaattttga cccgcttacg gctccccttt cgcattggtt taattgaagt ttataccagc
     6361 tgttcagtag gtattgcact ctctcccgaa catggttcgg acagcgcggc tattattcgt
     6421 cacgccgaca cagcgatgta cacagcaaag gaaggcggac gaggacaatt ttgcgttttt
     6481 accccagaaa tgaatcaacg ggtatttgaa tatctctggc tggataccaa cttgcgtaaa
     6541 gcactggaaa acgatcagtt ggttattcac tatcaaccga aaatcacctg gcgtggcgaa
     6601 gtgcgcagtc tggaagcact agtacgttgg cagtcacctg aacgtgggtt gattccaccg
     6661 ttggacttca tttcctacgc agaagagtca gggctaattg tgcctttagg ccgttgggtg
     6721 attctcgatg tcgtacgcca ggtggcgaag tggcgggata aaggcattaa cctgcgagtg
     6781 gcggtaaaca tttctgcacg tcagctcgcc gatcaaacca ttttcaccgc cctgaaacag
     6841 gttctccagg aactcaattt tgaatactgc cctattgatg ttgaattgac agagagttgt
     6901 ctgattgaga atgatgaact ggcactgtct gttattcaac aatttagcca actaggtgcg
     6961 caagtgcatc tggatgattt tggtaccggc tactcttcac tttcgcaact ggcgcgcttt
     7021 ccgatcgatg ccatcaaact tgaccaggtt tttgttcgag atattcacaa acaacctgtc
     7081 tcgcagtcac tggttcgggc aatcgtcgct gtggcccagg cattgaatct tcaggtgatc
     7141 gccgaaggtg tagagagtgc aaaggaagat gcttttttaa ccaagaacgg gatcaatgag
     7201 cggcaaggat ttttgtttgc caaaccgatg cccgccgtcg ccttcgaacg ctggtataaa
     7261 cgctatctga agcgcgcata agtagatgaa tatatatact tagcgctata ttccgtcatt
     7321 atatatcgta ttcccggatg ataattagcc gttgctgtta aacacttctc ttttagcggc
     7381 aatttttgca ttattcattt tccccctttt gctataagga tgtccttgat gtctgaattt
     7441 gatgcccagc gcgttgccga acgtattgat attgtgttag atatccttgt ggcaggcgat
     7501 taccactctg cgatccataa tctcgagata cttaaagcag aactcttgcg ccaggttgct
     7561 gaatctacgc cagacatccc gaaggcaccg tgggagattt aacaatcgtg ccttcaggtt
     7621 ttcctatttg aggatgcccg gcgcgtcagt attttttatt tagtattata acgttataag
     7681 aattacagcg atgaattccc gacaacaaac tattctacag atggtcattg accagggtca
     7741 ggttagcgta accgatctgg caaaagccac tggagtttct gaagttacca ttcgccagga
     7801 tctcaacacc ctcgaaaaac tgagttacct ccgccgtgca catggctttg cagtttcgct
     7861 tgatagtgat gacgtcgaaa cccgtatgat gagcaactat acgctgaagc gtgaactcgc
     7921 cgagtttgcc gcgtcactgg ttcaaccggg cgaaaccatc tttatcgaaa atggcagcag
     7981 caatgccctg ctcgcccgga ctctggggga gcagaagaaa aatgtcacta tcatcacggt
     8041 cagcagctac atcgcgcatt tgctgaaaga cgcgtcttgt gaagttattt tgctcggtgg
     8101 cgtgtaccag aaaaaaagcg aaagtatggt tggcccgttg acacgccagt gcatccaaca
     8161 ggtacatttc agcaaagcat ttattggtat tgatggctgg caacctgaaa ctggattcac
     8221 tggtcgcgat atgatgcgta ccgatgtggt gaatgccgtg ctggaaaaag agtgcgaagc
     8281 gatagtcctg actgacagct cgaaatttgg tgctgtacat tcatactcca tcggtcccgt
     8341 tgagcgattc aatcgcgtga ttaccgattc gaaaatacgc gccagcgatc tgatgcatct
     8401 tgagcacagt aaactcaccg ttcacgtcgt tgacatttaa ccatttccct tctctacgga
     8461 tgatttgcag tttggcaaat catccgctct aagatgattc ctggttgata attaagacta
     8521 tttacctgtt attaacactc tcaagatata aaattattat cagcgatata acaggaagtc
     8581 attatcacct gcgtgatata accctgcgcg cgagcggatt tcacggaata atttcaccag
     8641 gcttattctt agctattata gttatagaga gcttacttcc gtgaatcata aattcaggag
     8701 agagtattat gtttgtaacg agcaaaaaaa tgaccgcggc tgttctggca attaccctgg
     8761 caatgtctct gagtgcctgt tctaactggt ctaaacggga ccgcaacacc gcaatcggcg
     8821 cgggtgcagg ggcattaggc ggtgcagtac tgaccgatgg cagtacgttg ggtacattag
     8881 gtggcgcagc tgtcggtggt gttattggtc accaggtcgg taaataatcg taaataaatg
     8941 tcgattatcg actcgcaatt ataatacgct taattctgtt cagtacgaaa taactgaata
     9001 atacacacag taaaatattc gaaagccacg gatatatccg tggcttttta tgttaaccgc
     9061 ctgcgagttt taccttcatc cctttcgctt caagtagtga ttttaataaa tcacgcttat
     9121 cgccctggat ttcaataact ccatctttaa ctgctccgcc gcagccgcat tttttcttca
     9181 gttccgctgc aagttttgtc agttcggcat catcgagatc gataccggta atcaggcaaa
     9241 cgcccttacc tttacgtcca ctggtctgac gctgaatacg caccacacca tcgcctttag
     9301 ggcgtactgg ggccgctttt ggctcatcaa tacgtccggt atccgttgag tagaccagac
     9361 ggctgttgga atcactcatc atgcactccg ctgtaaagag gcgttgatcg ctttcagcgt
     9421 ctgcgctgga tctaccgatt gcgttaccgg gcgaccaatc accatataat caacaccagc
     9481 cgccaacgcc tgttctggcg tcataatgcg gcgctggtca cctgcttcac tcccctgcgg
     9541 acgaatgccc ggtgtaacca gtttgaactc ctgaccgaat acctgtttaa agcgcacagc
     9601 ttcctgagca gaacacacca caccatcaag gccacatttt tgcgtcagtg ccgccagacg
     9661 ttctgcataa tctgcaggtg acagtgtcac gccaagatcg gccaagtcgc tggcttccat
     9721 gctggtcaac actgtcacag caatcaaaag cggtgcatct ttgccaaacg gaaccagtgc
     9781 ctcacgcgct gcggtcatca tacgcgcccc accagaggca tgaacattca ccatccacac
     9841 gcctaagtca gctgcagcag cgacagcgtg cgctgcagtg ttggggatat cgtggaattt
     9901 caggtcaaga aagatatcaa aaccacgctg ttgaagttcg cgcacaaact gtggcccaaa
     9961 caatgtaaac atctctttac cgaccttcag acgacaatcg cgtgggtcga tcttgtcgac
    10021 aaaggccagc gcggcatcac gattatgata atcaagggca acaaccacag gagaattcgt
    10081 aacagcgcgg gaagaagatg aagcagttaa cgtcatgacc agaccttctt gatgatgggc
    10141 accggaagtg gcgcgaaaca ggtaaacggc gagcattcta cccgtggaaa taaaaaatta
    10201 acagttgcga tttctcccct ggcaggcttt ttctgcgccg ccgttgccgg agcacaatgg
    10261 gacataaata actagtatgt tgtaactaaa gtcggttttt ttaaaaaatt acaggccatc
    10321 aagaccgcga atcggtttaa tagttgacca ggcacgacaa gacggacaat gccagtagag
    10381 ggtgtatgcg gtaaaaccac atttctggca gcgataacga ggcttactac gtaccttctc
    10441 gccaaccatg tcacgcagca ccatcaggct ctctttggca cgcccttctt ccgcttcatt
    10501 taagtggtaa tccattaact tatggaacac acgcatggtc ggatgacgct gaagctggcg
    10561 cgtaatatag acctgtgcgg cctcactacc gtcgcgcgct tcgatgatat cagcaagcat
    10621 caattcagca tcggcaccgg tgttctcttc caccgcgcgc tgcaggaatt ctgcccattc
    10681 ggcagtttta cccaactgct gatagcaggt ttgcagcatt tccagcgttt cgctgaccag
    10741 ttctctgtcc tgggatatca cgcgttgcaa actttcgacg gctttggcgt attctccttt
    10801 cgccataaac acgcgtccca tcatgatgga tacgcgggcg ctgtttttat ctgccgccgc
    10861 cccttttttt agcaaggtca tggcacgatc gagatcgtcg ctggccatat gctgcagggc
    10921 taactcacag tagaaatggg caatttcgac gcgctgttta tctttaccca gcttcaccag
    10981 gcgttcggca acatcaattg ctttctgcca ctcactggta gcctggtaga tttgtagcaa
    11041 ctgttgcagc gcgccaatgc ggaagtcagt ttcatcggtc agctgattga acatgtcttc
    11101 cgcgcggtca tataacccgg cggccatgta atcacgcccc agttgttgaa tcgccaacag
    11161 acgctgttca taggtcagcg aggcgctttc cattagggtc tgatggatgc gaatagcgcg
    11221 atcaacttcg ccacgcgaac ggaacaggtt tccgagcgta aggtgggctt caacggtgcc
    11281 tgtatcctct ttaagcatat cgagaaacag gtctaccgct ttatcctgtt gattactaag
    11341 caggaagtta acccccgcta cgtaatcacg cgacaagcgg ttgacttcat cttgcttgtt
    11401 ttgttgcgca cttctgcggc ccatatacca gccataggca gcggctacag gcaaaagcag
    11461 aaacaacaac tccagcatag aaagttattc cttcgccgct gacgagtgct gcactacagc
    11521 cacgtcagtc gcgggtgaaa gctggttttc cagtcgcttt attttacgtt cagcgcgcac
    11581 cagggaaaca cgaactcgca gccagaacag gccacaaatc aaccaaccga tagcaaaacc
    11641 cgcagcaaac aataccgcca gcaatgtgga aatacggtac tccccttgcg ctaacagata
    11701 attaaacgtc acctgttgat cgttctgcgc acccaacgtg accgaaatca cgaagatcgc
    11761 taacaccagt aagaaaatga gtaaatattt cacattactt cccgttatgt ggttagagcg
    11821 aataaattat ggtcaactta ccgcaatagc cctttaaagt accattttcg cgccgtctgc
    11881 gaaatggcat ccacgcgcat tgttgatgat ttaagggcta aattcggaaa atcaaccagc
    11941 gttaactttc ttgttctcgt tgcgctattt cgcgattttc ttccgctggt ggtgttaatg
    12001 gcccacaaat tcgttgcgca agccacgttg ccaccgccac cagcgcccac gaaatcaacg
    12061 tagctactac cagatcgcgt ggccaatgca tcccgagcag caggcggctt cccatgactc
    12121 cagttgccca gaccaacaag atagcaatgg ttaacgttcg ccgacgcggc cacagcaaac
    12181 caacagccag cagtgcccaa ctggcagcaa acatcgtgtg accggaagga aaggcaaacc
    12241 ccgtctcttt ctgccagtgt gaacgcaaat attgtgggat atttttctct tcagccaact
    12301 gttctttcac tagatttccg cgttctgctc gctttaaagt gtagaactca tcaaccggaa
    12361 tatgatgtgt tttttccagc cagataacaa aaggtcgtgg ttcctggact ttgtctttga
    12421 tccaggattt aacgccttgt cccacaagga ttgcggccgc cagaatggca aataatacaa
    12481 aggcagcctt aatgcgaaaa cgcagacacc agagaaacca gccgaataaa atcaaatgtg
    12541 taatgacgcc ccagggctgg gtgacagttt cagtaaccca aaaagccgct tttagtagcc
    12601 aactttgttc tccaggttgc caacgccagc cagaaatcca tacggctact ggcataacaa
    12661 gcaatagtgc agctcccact gcggtacgtc tggcaatcga acgcatggcc tctccttttt
    12721 gataagtccc acaatcataa ctgaaaatgc cagttccagg aaaaattgac agatttgtgc
    12781 cattccgtga acgatcgacg cgtcgtgatt aggtgaaccc cttctcgtta tggcaaaata
    12841 agccaataca gaaccagcat tatctggaga atttcatgca gcttaaacgt gtggcagaag
    12901 ccaaactgcc aaccccatgg ggcgatttcc tgatggtggg atttgaagaa ctggcaaccg
    12961 gacacgatca tgtcgcgcta gtctatggcg atatttccgg gcataccccg gtacttgcgc
    13021 gcgtccattc cgaatgtctg accggtgacg ccctgttcag cttgcgctgc gattgtggct
    13081 tccagctcga agcggcattg acgcaaattg ccgaggaagg ccgtggtatt ttgctgtatc
    13141 accgtcagga aggtcgtaac attggtctgc tgaataaaat ccgcgcttac gcactgcagg
    13201 atcaaggtta cgatactgta gaggctaacc accagttagg cttcgccgct gatgagcgcg
    13261 acttcactct ttgcgctgat atgttcaaac tccttggcgt caatgaagtc cgcttgttaa
    13321 ccaataaccc gaaaaaagtc gaaattctga ccgaagcggg gattaatatt gttgaacgcg
    13381 taccattgat tgtaggtcgt aaccccaata acgaacatta tctcgatact aaagccgaga
    13441 aaatgggtca tttgctgaac aaataaccct cttgcattgt ggaattaatt tgcttgccgg
    13501 aagcaaaata accggcaagc aaatacttgt tacttcagca tattacgaat gacataatgc
    13561 aaaatgccgt cgttctggta gtaggtcaac tccgtcgcgg tgtcgatacg acaacggcag
    13621 ggtacgactt cctggctacc atccgcgcgc gtaagcgtca ccggaaccgt cgcgccgggt
    13681 tgtagatttt gcagatcgcc aatatcaatc ttctcttccc cggttagccc taacgtttta
    13741 cgcgtcacgc cttgcggaaa ttccagcggc aagatgccca tgccaattaa attcgaacgg
    13801 tgaattcgtt caaacgattc ggcaatcacc acacgaatac caagcagacg cggacctttt
    13861 gccgcccagt cacggcttga gcctgatcca tactctttcc cggcaatcac cgccagcggc
    13921 gtttgctcct gcttatagcg catcgcggca tcatagatag agacaacatc gctgtcaggt
    13981 aaatgccgcg tcatcccccc ttcaacgcca ggcaccattt cattacggat gcgaatattg
    14041 gcgaaggtgc cgcgcatcat cacttcatgg ttaccacgcc gcgaaccgta ggagttaaag
    14101 tcttttcgct caacaccccg accttgtaga tatcgacccg ccgggctgtc gggcttaata
    14161 ctgcccgccg gagagatatg gtcagtggtg actgaatccc ccagcattgc gaggatccgc
    14221 gcaccgtgaa tatcttccac aggtgctggt gttgcctgca tttcatcaaa gaaaggcgat
    14281 aaacgaatat aggttgaatc ctcctgccat ccgtaggtat cggatcgtgc gacgttaatt
    14341 tctttccact ctgcagtgcc ttcaaaaact tctgcgtact ctttgcggaa catttctgtg
    14401 gagacttgtt ctaccgcacg ggcaatttct tgtgccgatg gccagatatc tttcagataa
    14461 accggatcgc ctttgcgatc atggccgata ggctcagaag ccaggttgat attcatattt
    14521 cccgccagcg cataggcaac caccagcggc ggcgaggcca gccagttagt tttaaccagc
    14581 ggatggatac ggccttcaaa gttacggttg ccggacagca ccgcaccgac ggttaaatcg
    14641 ccttttttga ttgccgtttc gataggatcg ggcagcggcc cagagttacc aatacaggtg
    14701 gtacaaccgt atcccacaag gttaaacccc agttcgtcga gatacggtgt cagtttcgct
    14761 tttgccagat agtcagaaac aactttcgag cctggtgcca gcgacgcttt gacccacggt
    14821 tgccgcttga ggcccagagt tacggctttt ttcgccagca agcctgcagc catcagcaca
    14881 cttgggttag aggtgttggt gcacgaggtt atcgcagcaa tgaccacagc gccatcaggt
    14941 aactgatact gatgtccgtt cataacataa tcgaccggct ggcgatcttt atgcgtggca
    15001 ttcacttcca gttcgttact ggcggcaaat gcttttggta catcgggcag tgcaacgcga
    15061 tcctgtgggc gttttggccc tgccaggctc gcttcaacgt cattcatatc cagttctaac
    15121 gtactggtaa aaatcggttc atcgcccggg ttacgccaca tgccctgcgc tttggcgtat
    15181 ttttcgacca actcgacctg atcttcactg cgcccgctta aacgcatgta atcgagggtg
    15241 acagcatcga ttgggaagaa gccacaggtg gcaccatatt ctggcgacat attggcaatg
    15301 gtggcgcgat ccgccaacgg tagtgaatcc agaccatcac cataaaattc gacgaatttc
    15361 ccgaccacgc catgtttgcg cagcatttgg gtaacagtga gaaccaggtc tgtggcggta
    15421 ataccttcac gtaattttcc ggtaagtttg aagcccacta cgtccgggat aagcatggaa
    15481 accggctggc ctaacattgc ggcttctgct tcgatcccac caacgcccca ccccagcacg
    15541 ccaaggccgt tgatcatggt ggtgtgcgag tcagtaccaa cgagtgtatc cggataagca
    15601 atccattcac cgtcctgcaa ttcactccac actgctttgc cgagatattc gaggttaacc
    15661 tgatggcaaa tgcctgtgcc tggcggcacg acgctaaacc gactgaacgc ctgctttccc
    15721 catttcagga acacataacg ttcgtggttg cgctccattt ccaggcgtac gttttcttca
    15781 aatgcctcat catcaccaaa acgatcgaca gtcaccgagt ggtcaatgac caggtcgacc
    15841 ggtgagagcg gattaacctt tgcagtatcg ccgccgaggc gtttaaccgc ttcgcgcatt
    15901 gccgccagat caacaacggc aggtacgccg gtaaagtcct gcatcagcac ccttgccggg
    15961 cggtaggcaa tttcacggtc agcatgggca tttttcagcc atcccgccag cgcgtggata
    16021 tcctcttcgg taaccgagtt accatcctgc cagcgcagca ggttttcgag caaaactttg
    16081 agtgacttgg gtagacgggt gatatcgccc agtgatttgg cagcaagcgg caggctgtag
    16141 tagtggtaag ttttatcttt ggcctgcaac gcgtccttac tggcttctcg tagggttgac
    16201 gacatagctc ctccttaatg acagggttgc gaagccctga ttctcttcag ggtgaatatt
    16261 aaagataaca caaacatcgc gtaacgattt gataacaacc caaattgata aaagaggaga
    16321 aaccttgtgg caaagcaaat gacaaccccg ccgcagcggg gtcaaggatc agtgagtaat
    16381 gatccagagg agttcggccc agaagagaat cgacagagaa aatgcaccca tccaggacca
    16441 atatttcata tttgtgttga tcataggcta cacccttaac ggttattact ttgtgttgag
    16501 ataactcaac ctgttattgc tgcggtgtat taaaagccct ttaattcatt aaaaatgggt
    16561 aataagaatg atgataatta ttgtttcggc tgatcatctt cagcaaacag ttctataaag
    16621 gcttgctgct gcgatgttag ttgccagtcg tctggaataa cgacggaaca ttcccgtttc
    16681 gtttctggtt cattgtcact atcctggcat aatggtgacg actccagttg ctcctgacca
    16741 accatagtta tccccacact ttccaaatca gcagtgcgac aaccacccag aaaagtgcag
    16801 aaccaagaaa aaccgccagc caggctttac gttttaattc aggatcgcgg cggggttcct
    16861 gatttccaga aggcattgct aacctcatat aatcgccatc acttatcagc aagacgttga
    16921 acgattggtg taattaatca tacgataaga caaatcctaa agaaacttta gtcaaaaatc
    16981 cagcgaattt acgaatcgtc ttcagtgaat aattcaatct tttgacctga aatagatatt
    17041 tgaaaactgg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
    17101 aaatatgagg gataaaaata ttgtgcacgg tatacaaata agtaacaaaa gagaatatat
    17161 tacggcacct tcgctacata aaaggtgccg ataataacgc aagaaattat ttttccggca
    17221 gttttatatc tttaaacatg acctcaattt cttcattgga acgcaatgcg acagccgcat
    17281 caacgacatc acgcgttaaa tgtggtgcaa aacgctgaat gaaatcatac atataactgc
    17341 gcaaaaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
    17401 catcaacgcg cacaaggtcc ggatcggcga ccggatccac cgccatgctg gcaatgaccc
    17461 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaaa
    17521 cgatacgcgg cgttaaccct gcgcgattaa aggcagtatc cagttctgaa cgtccggtaa
    17581 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
    17641 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
    17701 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa tctgcattac
    17761 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
    17821 ggggataacg ctcaataaag cctttgatga cgtttggtaa cgcgtagcgt gcctgggtat
    17881 gcgtggtggc gatatacagc gagcctttat ccggccaggt gtgctcaccg gcaaccgatt
    17941 ttatggcatc aactttcgac aggacttcgc gagcgatacg aattatttct tgtcctgctg
    18001 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
    18061 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
    18121 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
    18181 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
    18241 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
    18301 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
    18361 ttgtttataa aaacctgaca gaattaaagg ttattttttc ccttcaaccc atttgccatc
    18421 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
    18481 tttggtttta cggctaaagc gaaccatagt cttattacct tccggatcct gctgcggcgc
    18541 atcggccaga taacgcagtt tttccggcag acgatcgcgg aagcgataca gctcttccac
    18601 cagcggcgca cgcgtttcac gcgatttcgg gaaggtattg gccgccagga acacaccggc
    18661 agcaccgtca cgcagcacga aataggcatc tgatttttcg cacggcagct ccggtaatgg
    18721 taccggatct tctttcggtg gagccacttc gccgttacgt aaaatcttac gcgtgttttt
    18781 acactcttcg ttggtgcagg ccatatattt accgaaacgc cccattttca ggtgcatttc
    18841 agaaccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
    18901 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
    18961 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
    19021 cagcgcgttg gtttcagcgt cttcgccttc cagcacgttc agcacttcgt tttccggcac
    19081 caggttaatg gttgttttgc aacgctcttt cggcggcagc gcatagccag aacagccaag
    19141 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaggtcg ggcagtcgat
    19201 gctggtcaga accatctggt tcggacgcat acccccctct tccggatctt tttcagcttt
    19261 atctaactgc tgagtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
    19321 gtgatttgcc acctggtcga ggctgttttc catctgcgcg gtgaaatcgt agttcattaa
    19381 ctcgcggaaa ttctcttcca ggcgatcggt aacgatttcg cccatttttt ccgcatagaa
    19441 acgacggttt tctacacgca catagccacg gtcctgaatg gtcgaaatga tcgacgcata
    19501 ggtagacgga cgaccgatac cgcgtttttc aagctcttta accagcgatg cttcactgaa
    19561 acgggctggc ggtttggtaa agtgctgggc tggtgtcagt tcaacgagcg tcagagcatc
    19621 gcctttatcg actgccggta agatacgatc ttcatcgcct ttacgcagtg caggcatcac
    19681 ttttgtccag ccatcaaagc gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
    19741 accaacggtc agcgtcgtgg agtcatattt cgctggggtc atctggcaag caacgaactg
    19801 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca acgattccgc
    19861 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
    19921 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
    19981 gcgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
    20041 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
    20101 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
    20161 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
    20221 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
    20281 caacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
    20341 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcggactg ccaccgactg
    20401 cacacgaccg gcagatagac cacgagcgat ctttttccat agcagcggcg aaaccatata
    20461 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
    20521 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
    20581 tcggctatag cgcgcatcat caccaccaat cacttcccgc aggtgccatg caatggcttc
    20641 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
    20701 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
    20761 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
    20821 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
    20881 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
    20941 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
    21001 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
    21061 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
    21121 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
    21181 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
    21241 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
    21301 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
    21361 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
    21421 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
    21481 gcgtaatggc gtgctggtat ttaccggcga ctacttttta gatgaacaag gattgccaac
    21541 agccaaaagc accgcggtgt ttaatatgtt caaacacttg gcccatgtgc tttcagaaaa
    21601 atatcacctg gttgattaaa gcaaaacgcg aggtcttacc ctcgcgtttg tcttttacat
    21661 caatggcttt tgcccacgct gccaccagcg tagcaataat cgatctgcgc tctctgccgc
    21721 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat tgaccacttc
    21781 tcggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
    21841 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
    21901 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
    21961 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tacgcttata
    22021 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
    22081 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
    22141 gtccgccaca caggccatca tgtaaccgcc gctggcagca actttgtcta ccgtaacagt
    22201 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
    22261 caccatgcca ccagggcttt ccagacgtag tacaacctga tcctgcggtt tgaatgctgc
    22321 gagtacagcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
    22381 aaaatccagc acccagacgc ggggtttact gtcagttgcc acctctccca gtttggcttt
    22441 cgcttttgct gctttcgctt cttgcttatg ctttttcttc tgcgctttgt gccactgttt
    22501 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
    22561 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
    22621 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
    22681 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
    22741 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
    22801 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
    22861 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
    22921 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
    22981 gccagtgatg gtattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
    23041 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
    23101 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcaggtc cgaagattgc
    23161 caacaactgg cacagcgcat tgccgttaat tatccgcgtc tggatggtgt tttgcataat
    23221 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccacaggt ctggcaggac
    23281 gtcatacagg tcaacgttaa tgctaccttt atgctcaccc aggcactgct tcctttatta
    23341 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg tcagggacga
    23401 gccaactggg gcgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
    23461 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
    23521 gcaatgcgtg ccagtgcatt cccgaccgaa gatccacaga aactaaaaac acccgctgat
    23581 atcatgccgc tctacctttg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
    23641 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
    23701 agcgtcagca acgagtgaaa gagaaagtag atgctcgtgt ggcccaggcc caggatgaac
    23761 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
    23821 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
    23881 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
    23941 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
    24001 tctggcaaca tgcaaagcgg atgcttgccg atccttcact ggatatggtt ttgctcgatg
    24061 agctgacata tatggtggcg tatgactatt tgccactgga agaagtgttg caggcgttaa
    24121 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
    24181 ttgaactggc ggatacggtg agtgagttgc gccccatcaa acatgcgttt gatgccggtg
    24241 taaaagcgca gatagggatc gattattaat ctgtaaaaat gccggatatg acgtccggca
    24301 tactttgatt aaccgttatt acgtccgcca gaacgacgac cgccgctcac ctgactgtga
    24361 cgtttcaccg cacgacgaat ctgattcgcc ttcatgcgac gacggtcttt ttctacagcg
    24421 actttagagc tggtttctgg cggtagctcc accagttcgc gcagatagtt agtctgggcg
    24481 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
    24541 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
    24601 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
    24661 aacttgatgg ttttaaaagc tgccggacca tcttccaact gcacgccacg actcaaatca
    24721 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg catattcacg ttcaacttca
    24781 cggcttgggt gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
    24841 caggtattaa cgtccaagcg acccacggca atccagcgtg cgccacgcag ttttggcaga
    24901 cggtcaaaca ccgttggacg tccttccgga tcgttacggg tacacaactc accttccggt
    24961 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcaggtga
    25021 ccatcgatac ggattttcaa tccaggggta acttcaacgc gatcgccgag tttagcaatt
    25081 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgagaaccg
    25141 tggccggcac gcgccagcac tttctgtagc ttttcgctca tagagcttcc ttttggtgtc
    25201 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
    25261 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
    25321 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
    25381 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgccg ccagcagcac
    25441 caccggaata acttcgcccc aacaaactgg tattggcccc cagactcagg tgcatctgta
    25501 atgagaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
    25561 gggtgatacg tgctacccat tctcttccaa ggtttgctga gacagcccaa cctggcaacg
    25621 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
    25681 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttggg
    25741 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
    25801 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atatagagag
    25861 aataaaaggc cacgcctgaa caaatcagcg gtaaaaacaa agtgatgatt acacgattaa
    25921 cgcaaggctt gtcatcatcg cgggcaaaca aaagctcaag ggaacaaaat aaaaaaggaa
    25981 gaagccagca gatcataata aacatatcgc ccaccagact cattcctgcc cagcctacag
    26041 gactcaggtt accgtagaga atgaccgcca atattgccag tccaccccat tgcaaaaacg
    26101 tatcaggccg gtaaaacgaa gactttttac gcggcagata aaatttctgc atccgtaaaa
    26161 atgccctgcg taagactctg ttgcgtctcc ccgtaagaac gaccttatcg cctttacgaa
    26221 ataacgcttc cagtaataac tgctctgcgg gcagtaatac cgcattattt ctggtcacgc
    26281 cttcattaac acgaatcagg gtttgctggt tcttgtcaga ctgatcttcc agatgaattt
    26341 ttcctttcac tatcagattt acgatatcgg cacaaaaacc tttatcgtcg taaaccagct
    26401 tcgcatcgag acgtaacatg ccgggcgtgt aatctggcgg aatgacatcg gtttcaatca
    26461 catctaccgg cgtaaattgc ggcctgcgct tccagagata taaccatcca caaaccagga
    26521 acagacacgg aaaccaaatt aacaacgatg acgtggaggg taaaagaaga tggctgaaaa
    26581 tattcgtcgt ttgtggagcc ggggcattgc taagtaacga gtgaggccaa cggtagagga
    26641 ccgtgaaatc ttcacgataa aacggatcgc tggattcaat tcttccgtcc tcaaggatgc
    26701 ggccatttcg atatgtgtcg ccctcttctc cagtaaagag atcgatttcg ctaaatggat
    26761 tacctgcgat atcagggaac ttgagtgaaa atcgggtctt atagatttca aacggccagt
    26821 ggttaccagt cacgttccag attaacagat ctgaatctcc ctcacgcagg aaagcatttt
    26881 taacctgata atgaatttga taatgatagt cgccttgggg caagaaacgt tgtttatcgc
    26941 ccacgacaat actcattaac ccgatatgcc agtcaagatg ccagggttca ggaataccat
    27001 cacgggttac gccaacaata tgatagttca gcagtgcagc gtccccatta ggctgcatcc
    27061 atagtcgtgg taaatcacga taaaatccgt gcttaatctg ctgatttcgc gcctgaaaat
    27121 gaatgttctc tttgatgtcg agcaccccat ccggttgcag agtggcttca acatcaaaga
    27181 gcgagatgcc ttcttcataa gaaggcatct ccgtgttttt cgcataactt aacgaagaga
    27241 aaaaaagccc aactatcaat agcaaaatgc agcgaaactt ccctgccata gtcttccatc
    27301 cgtggaacgc aatttgttat aagaaaggct tcacatcacc tacgccttca cgcaccacga
    27361 ccggcgtatc atcggtaaga tcaataaccg ttgttggttt ctgcccgaga tagccaccgt
    27421 gaataatcaa atccacctgt ttttccagac ggtctttaat ttcttccgga tcggattcgg
    27481 taaattcgct gcctggcagc attagcgaag tggaaagcat cggttcgccc aatgcttcaa
    27541 gtaacgcctg cgcaatcggg ttagacggca cacgcatacc gatggttttg cgtttttcct
    27601 gcaacagacg gcgtggcact tctttcgtcc ctttcaggat gaaggtgtag ttacccggcg
    27661 tgttgttttt catcaaacga aacgcaacgt tatccacaaa tgaataggtc gagagttcag
    27721 aaagatcgcg acacatcagg gtaaagttgt gaccgtccgg cagctggcga atacgacaaa
    27781 tacgctccat ggcgttttta tcttcaattt tacagccgag cgcatagccg gaatcagttg
    27841 gataaacaat caccccgcct ttgcgcacga tctccaccgc ctggttgatc agacgttgct
    27901 gtgggttatc aggatgaata taaaaaaact ggctcataat tccctctctg tggtgttctg
    27961 cggctgttcc catagctgcc agacgccttc aacgcctgcg ggcagccaga gtttacgacc
    28021 cagttcgatc cacgggcatg gctgatgaaa atcagatcct tgtgatgccc ataaatgatg
    28081 ctgacgcgca agtgtcgcca gctgggtgcg ttcattgggc gattgctggc actgcgcgac
    28141 ttccatcgcg tcgccgtcgt gttcggcaaa atgcgcaacc aatcttttca gccatttagc
    28201 actaagattg taccgcccag gatgagctag caccgcctta ccgccagaat gatgaatgac
    28261 atcaatagct tgttctattg tacaccactg tggcggaacg tatccggttt tcccgcgcgc
    28321 cagatacttt ttaaagacat ccgccattga actggctttg ccgcactcaa caaggaaacg
    28381 cgcaaaatga ccgcgcgtca ctgcgccgcc ctgcgccagt cgttgtgcgc cttccagcgc
    28441 cccaggtatt tgcgcttttt caagtctttc ggcaatttgc tgcgcccgct gattgcgccg
    28501 ttctgtctgc tgcgcgagga actcacacat tagcggatga gtaatatcaa tattcagccc
    28561 gacaatatga atttcatgat tttcccagac cgtggaaatt tccacgccgg gaataagatt
    28621 cagcgccagt cccgaacgtg aaatttcctc tcttgctggc gcgattgcag ctgtggtgtc
    28681 atggtcggtg atcgccaggg tgccgacgcg catctcgact gcacggtgca ccaatgcttc
    28741 tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcgta
    28801 attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa
    28861 cggttctggc aaatattctg aaatgagctg ttgacaatta atcatcgaac tagttaacta
    28921 gtacgcaagt tcacgtaaaa agggtatcga caatgaaagc aattttcgta ctgaaaggtt
    28981 ggtggcgcac ttcctgaaac gggcagtgta ttcaccatgc gtaaagcaat cagataccca
    29041 gcccgcctaa tgagcgggct tttttttgaa caaaattaga gaataacaat gcaaacacaa
    29101 aaaccgactc tcgaactgct aacctgcgaa ggcgcttatc gcgacaatcc caccgcgctt
    29161 tttcaccagt tgtgtgggga tcgtccggca acgctgctgc tggaatccgc agatatcgac
    29221 agcaaagatg atttaaaaag cctgctgctg gtagacagtg cgctgcgcat tacagcttta
    29281 ggtgacaccg tcacaatcca ggcgctttcc ggcaatggtg aagccctgct gacactactg
    29341 gataccgccc tgcctgcggg tgtggaaaat gaacaatcac caaactgccg tgtgctgcgc
    29401 ttcccccctg tcagtccact gctggatgaa gacgctcgct tatgctccct ttcggttttt
    29461 gacgctttcc gcttattgca gaatctgttg aatgtaccga aggaagaacg agaagcaatg
    29521 ttcttcggcg gcctgttctc ttatgacctt gtggcggggt ttgaagattt accgcaactg
    29581 tcagcggaaa ataactgccc tgatttctgt ttttatctcg ctgaaacgct gatggtgatt
    29641 gaccatcaga aaaaaagcac ccgcattcag gccagccttt ttgctccgaa tgaagaagaa
    29701 aaacaacgtc tcactgctcg cctgaacgat cttcgccagc aactgaccga aaccgcgcca
    29761 ccgctgccgg tggtttccgt gccgcatatg cgttgtgaat gtaatcagag cgatgaagag
    29821 ttcggtggcg tagtgcgttt gttgcaaaaa gcgattcgcg ctggagaaat tttccaggtg
    29881 gtgccatctc gccgtttctc tctgccctgc ccgtcaccgc tggcggccta ttacgtgctg
    29941 aaaaagagta atcccagccc gtacatgttt tttatgcagg ataatgattt caccctgttt
    30001 ggcgcgtcgc cggaaagttc gctcaagtat gacgccacca gccgccagat tgagatctac
    30061 ccgattgccg gaacacgtcc acgcggtcgt cgcgccgatg gcgcgctcga cagggacctt
    30121 gacagccgta ttgaactgga aatgcgtacc gaccataaag aactttcaga acatctgatg
    30181 ctggtagatc tcgcccgaaa tgacctggcg cgcatttgca cccccggcag ccgctacgtt
    30241 gccgatctca ccaaagtaga ccgttattcc tatgtgatgc acctcgtctc ccgagtggtc
    30301 ggggaactgc gccacgatct cgacgccctg catgcttacc gcgcctgtat gaatatgggg
    30361 acgttaagcg gtgcgccgaa agtacgcgct atgcagttaa ttgccgaggc tgaaggtcgt
    30421 cgccgcggca gctacggcgg cgcggtaggt tatttcaccg cgcacggcga tctcgacacc
    30481 tgcattgtga tccgctcggc gctggtggaa aacggtatcg ccaccgtgca agccggtgct
    30541 ggcgtagtcc ttgattctgt tccgcagtcg gaagccgacg aaacccgtaa taaagcccgc
    30601 gctgtactgc gcgctattgc caccgcgcat catgcacagg agactttctg atggctgaca
    30661 ttctgctgct cgataatatc gactctttta cgtacaacct ggcagatcag ttgcgcagca
    30721 atgggcataa cgtggtgatt taccgcaacc atattccgga gcaaacctta attgaacgcc
    30781 tggcgacgat gagcaatccg gtgctgatgc tttctccagg acccggtgtg ccgagcgaag
    30841 ctggttgtat gcctgaactg cttacccgcc tgcgcggtaa gctgcctatt attggcattt
    30901 gccttggtca tcaggcaatt gtcgaagctt acgggggcta tgtcggtcag gcgggcgaaa
    30961 ttctccacgg taaagcctcc agcattgaac acgacgggca ggcgatgttt gccggattaa
    31021 caaacccgct gccggtggcg cgttatcact cgctggttgg cagtaatatt ccggccggtt
    31081 taaccatcaa cgcccatttt aatggcatgg tgatggcggt gcgtcacgat gcagatcgcg
    31141 tttgcggttt ccagtttcat ccagagtcca ttcttactac ccaaggtgct cgcctgctgg
    31201 aacaaacgct ggcctgggcg cagcagaaac tagagccaac caacacactg caaccgattc
    31261 tggaaaaact gtatcaggcg cagacgctta gccaacaaga aagtcaccag ctgttttcag
    31321 cggtggtgcg tggcgagctg aagccggaac aactggcggc ggcgctggtg agcatgaaaa
    31381 ttcgcggtga gcacccgaac gagatcgctg gggcagcaac cgcgctactg gaaaacgcag
    31441 cgccgttccc gcgcccggat tatctgtttg ctgatatcgt cggtactggc ggtgacggca
    31501 gcaacagtat caatatttct accgccagtg cgtttgtcgc cgcggcctgt gggctgaaag
    31561 tggcgaaaca cggcaaccgt agcgtctcca gtaaatctgg ttcgtcggat ctgctggcgg
    31621 cgttcggtat taatcttgat atgaacgccg ataaatcgcg ccaggcgctg gatgagttag
    31681 gtgtttgttt cctctttgcg ccgaaatatc acaccggatt ccgccacgcg atgccggttc
    31741 gccagcaact gaaaacccgc accctgttca atgtgctggg gccattgatt aacccggcgc
    31801 atccgccgct ggcgttaatt ggtgtttata gtccggaact ggtgctgccg attgccgaaa
    31861 ccttgcgcgt gctggggtat caacgcgcgg cggtggtgca cagcggcggg atggatgaag
    31921 tttcattaca cgcgccgaca atcgttgccg agctgcatga cggcgaaatt aagagctatc
    31981 aattgaccgc tgaagatttt ggcctgacgc cctaccacca ggagcaactg gcaggcggaa
    32041 caccggaaga aaaccgtgac attttaacac gtttgttaca aggtaaaggc gacgccgccc
    32101 atgaagcagc cgtcgctgcg aacgtcgcca tgttaatgcg cctgcatggc catgaagatc
    32161 tgcaagccaa tgcgcaaacc gttcttgagg tactgcgcag tggttccgct tacgacagag
    32221 tcaccgcact ggcggcacga gggtaaatga tgcaaaccgt tttagcgaag atcgtcgcag
    32281 acaaggcgat ttgggtagaa gcccgcaaac agcagcaacc gctggccagt tttcagaatg
    32341 aggttcagcc gagcacgcga catttttatg atgcgctaca gggtgcgcgc acggcgttta
    32401 ttctggagtg caagaaagcg tcgccgtcaa aaggcgtgat ccgtgatgat ttcgatccag
    32461 cacgcattgc cgccatttat aaacattacg cttcggcaat ttcggtgctg actgatgaga
    32521 aatattttca ggggagcttt gatttcctcc ccatcgtcag ccaaatcgcc ccgcagccga
    32581 ttttatgtaa agacttcatt atcgaccctt accagatcta tctggcgcgc tattaccagg
    32641 ccgatgcctg cttattaatg ctttcagtac tggatgacga acaatatcgc cagcttgccg
    32701 ccgtcgccca cagtctggag atgggtgtgc tgaccgaagt cagtaatgaa gaggaactgg
    32761 agcgcgccat tgcattgggg gcaaaggtcg ttggcatcaa caaccgcgat ctgcgtgatt
    32821 tgtcgattga tctcaaccgt acccgcgagc ttgcgccgaa actggggcac aacgtgacgg
    32881 taatcagcga atcaggcatc aatacttacg ctcaggtgcg cgagttaagc cacttcgcta
    32941 acggttttct gattggttcg gcgttgatgg cccatgacga tttgaacgcc gccgtgcgcc
    33001 gggtgttgct gggtgagaat aaagtatgtg gcctgacgcg tgggcaagat gctaaagcag
    33061 cgtatgacgc gggcgcgatt tacggtgggt tgatttttgt tgcgacatca ccgcgttgcg
    33121 tcaacattga acaggcgcag gaagtgatgg ctgcggcacc gttgcagtat gttggcgtgt
    33181 tccgcaatca cgatattgcc gatgtggtgg acaaagctaa ggtgttatcg ctggcggcag
    33241 tgcaactgca tggtaatgaa gatcagctgt atatcgatac gctgcgtgaa gctctgccag
    33301 cacacgttgc catctggaaa gcattaagcg tcggtgaaac cctgcccgcc cgcgagcttc
    33361 agcacgttga taaatatgtt ttagacaacg gccagggtgg aagcgggcaa cgtttcgact
    33421 ggtcactgtt aaacggtcaa tcgcttggca acgttctgct ggcggggggc ttaggcgcag
    33481 ataactgcgt ggaagcggca caaaccggct gcgccggact tgattttaat tctgctgtag
    33541 agtcgcaacc gggcatcaaa gacgcacgtc ttttggcctc ggttttccag acgctgcgcg
    33601 catattaagg aaaggaacaa tgacaacatt acttaacccc tattttggtg agtttggcgg
    33661 catgtacgtg ccacaaatcc tgatgcctgc tctgcgccag ctggaagaag cttttgtcag
    33721 cgcgcaaaaa gatcctgaat ttcaggccca gttcaacgac ctactgaaaa actatgccgg
    33781 gcgtccaacc gcactgacca aatgccagaa cattacggct gggacgaaaa ccacgctgta
    33841 tctgaagcgc gaagatttgc tgcacggcgg cgcgcataaa actaaccagg tgctcggtca
    33901 ggctttactg gcgaagcgga tgggtaaaac cgaaatcatc gccgaaaccg gggccggtca
    33961 gcatggcgtg gcgtcggccc ttgccagcgc cctgctcggc ctgaaatgcc gtatttatat
    34021 gggggccaaa gacgttgaac gccagtcgcc aaacgttttt cgtatgcgct taatgggtgc
    34081 ggaagtgatc ccggtgcata gcggttccgc gacgctgaaa gatgcctgta acgaggcgct
    34141 acgcgactgg tccggcagtt atgaaaccgc gcactatatg ctgggcaccg cagctggccc
    34201 gcatccttat ccgaccattg tccgtgagtt tcagcggatg attggcgaag aaaccaaagc
    34261 acagattctg gaaagagaag gtcgcctgcc ggatgccgtt atcgcctgtg ttggcggcgg
    34321 ttcgaatgcc atcggcatgt ttgcagattt catcaacgaa accaacgtcg gcctgattgg
    34381 tgtggagcct ggcggtcacg gtatagaaac cggcgagcac ggcgcaccgt taaaacatgg
    34441 tcgcgtgggc atctatttcg gtatgaaagc gccgatgatg caaaccgaag acgggcagat
    34501 tgaagaatct tactccatct ccgccgggct ggatttcccg tccgtcggcc cacaacacgc
    34561 gtatcttaac agcactggac gcgctgatta cgtgtctatt accgatgatg aagcccttga
    34621 agccttcaaa acgctgtgcc tgcacgaagg gatcatcccg gcgctggaat cctcccacgc
    34681 cctggcccat gcactgaaaa tgatgcgcga aaacccggaa aaagagcagc tactggtggt
    34741 taacctttcc ggtcgcggcg ataaagacat cttcaccgtt cacgatattt tgaaagcacg
    34801 aggggaaatc tgatggaacg ctacgaatct ctgtttgccc agttgaagga gcgcaaagaa
    34861 ggcgcattcg ttcctttcgt cacgctcggg gatccgggca ttgagcagtc attgaaaatt
    34921 atcgatacgc taattgaagc cggtgccgat gcgctggagt taggtatccc cttctccgac
    34981 ccactggcgg atggcccgac cattcaaaac gccactctgc gcgcctttgc ggcaggtgtg
    35041 actccggcac aatgttttga aatgctggca ctgattcgcc agaaacaccc gaccattccc
    35101 attggcctgt tgatgtatgc caatctggtg tttaacaaag gcattgatga gttttatgcc
    35161 cagtgcgaga aagtcggcgt cgattcggtg ctggttgccg atgtgccagt tgaagagtcc
    35221 gcgcccttcc gccaggccgc gttgcgccat aatgtcgcac ctatctttat ctgcccgcca
    35281 aatgccgatg acgacctgct gcgccagata gcctcttacg gtcgtggtta cacctatttg
    35341 ctgtcacgag cgggcgtgac cggcgcagaa aaccgcgccg cgttaccact caatcatctg
    35401 gttgcgaagc tgaaagagta caacgctgca cctccattgc agggatttgg tatttccgcc
    35461 ccggatcagg taaaagcagc gattgatgca ggagctgcgg gcgcgatttc tggttcggcc
    35521 attgttaaaa tcatcgagca acatattaat gagccagaga aaatgctggc ggcactgaaa
    35581 gcttttgtac aaccgatgaa agcggcgacg cgcagttaat cccacagccg ccagttccgc
    35641 tggcggcatt ttaactttct ttaacgaagc cggaaaaatc ctaaattcat ttaatattta
    35701 tctttttacc gtttcgctta cccctgtcaa acgtcaactt acgtcatttt tccgcccaac
    35761 agtaatataa tcaaacaaat taatcccgca acataacacc agtaaaatca ataattttct
    35821 ctaagtcact tatttctcag gcaattgtta atatatcaag aacgttcctc ataatatatt
    35881 ttcccacttt attctcgtta cgcttaattt gactaattct cattggcgac taattttaat
    35941 gagtgtcgac acacaacact catattaatg aaacaatgca acgcaacggg agaaataaca
    36001 tggccgaaca tcgtggtggt tcaggaaatt tcgccgaaga ccgtgagaag gcatccgacg
    36061 caggccgtaa aggcggtcag catagcggcg gtaattttaa aaatgatccg caacgcgcat
    36121 ctgaagcggg taaaaaaggc ggtcaacaaa acggtggtaa taaatcaggt aaatcctgat
    36181 tcaccgttaa ttattaatat cagctgataa agataattgc atctgcgggc cgtatggctc
    36241 gcagtgattc cactggagaa aaatatgaat atgaagacca ttgaagatgt atttattcac
    36301 ctgctttcag atacctacag cgcagaaaaa caattaaccc gggcactggc aaaactcgca
    36361 agagcaacat caaacgaaaa attaagtcag gcttttcatg cgcacctcga ggaaacacat
    36421 ggacagattg aacgtattga tcaagttgtg gaatcagaat cgaatctgaa aattaagcgc
    36481 atgaaatgtg tggcaatgga aggtcttatt gaagaagcta atgaggtcat cgaaagtacc
    36541 gagaaaaacg aagtgcgtga tgccgcactg attgccgcag cacagaaagt cgagcattat
    36601 gagattgcca gttacgggac attagcgacg ctggctgaac aattaggtta ccgtaaagca
    36661 gcgaagcttc tgaaagagac cctggaagaa gaaaaggcca ccgacatcaa actgactgat
    36721 ctggccctta ataacgtaaa taagaaagcc gaaaataaag cctgaaatat gaactttaac
    36781 ttttagtcat tttataaaga ggacattttc atgaatcgta ttgaacatta tcatgactgg
    36841 ttacgtgacg cccatgcaat ggaaaagcaa gccgaatcta tgcttgaatc catggccagc
    36901 cgtatagata attatcctga actacgtgct cgtattgaac aacatcttag tgaaaccaaa
    36961 aaccagattg ttcaactgga aactattctt gatcgtaatg acatttcacg ttcagtcatt
    37021 aaagattcca tgagtaaaat ggctgcgctt gggcagtcaa tcggtggtat attcccttct
    37081 gatgaaatag tcaaaggctc tattagcgga tatgtcttcg agcaatttga aatcgcctgt
    37141 tacacctctc tattagcagc agcaaaaaat gccggtgata cagcctcaat tccgatcatc
    37201 gaagcgattt taaatgagga aaagcaaatg gccgactggc tgattcagca tattccgcaa
    37261 acaactgaga aatttttaat tcgctctgaa actgatggcg tagaagcgaa gaaataatac
    37321 cctttatgcc atgtccttat tgaccccgta tattacgggg tcgtttttgt gcggaattaa
    37381 aaacgatatc ctgctgagaa cataaacacc cacggatcga ggcgtacgct atcatgttgc
    37441 tgtgcaccgc ccagtttata attggcggtg gtatcgatat ccatgtacca gactgacatg
    37501 ttaaccaacc agtcacggtt aatcagataa tcaaccccca cctgcccggc agctccccag
    37561 gaatctttca gactgagatc ggaaagcccc gcctctttgc catgatcgtt aaatccatta
    37621 tcaaagaagg tggtgtagtt aatacctgcc ccaacataag gacggaattt gctgctggca
    37681 tcaccaaaat accactgcgc catcagtgtt ggcggcagat gatgaacggt tgcaatatcg
    37741 ccggtcgccc gggtgccgat tttatggcgg aacggcgtcg ctgccagtaa ttccacacca
    37801 atgttgtcgg tcgccatata agtaaacgtc aggcccagtt gagtgttatt ggtcacgctg
    37861 aatccaccca gacttcctaa cgtaccacca gcaccttctg ttggacgtac ggttgcagaa
    37921 cctgcacgca taaaaaattc gcctgcttca tgcgcaaagg cactgccaga gagaagagtt
    37981 gttactgcca aagccgccac tgttaacttt ttcatatccg ctccgtcgtt atggttataa
    38041 aaacgtgacg aatataccta cattggagta acaaatgat
//
LOCUS       DAZFFG010000031        37990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFFG010000031 DAZFFG010000000
VERSION     DAZFFG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.031"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(91..378)
                     /gene="yebO"
                     /locus_tag="ACHJ7J_001974"
     CDS             complement(91..378)
                     /gene="yebO"
                     /locus_tag="ACHJ7J_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO5000211.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
     gene            complement(453..596)
                     /gene="mgrB"
                     /locus_tag="ACHJ7J_001975"
     CDS             complement(453..596)
                     /gene="mgrB"
                     /locus_tag="ACHJ7J_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO5000212.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            755..994
                     /gene="yobH"
                     /locus_tag="ACHJ7J_001976"
     CDS             755..994
                     /gene="yobH"
                     /locus_tag="ACHJ7J_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO5000213.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1138..1929)
                     /gene="kdgR"
                     /locus_tag="ACHJ7J_001977"
     CDS             complement(1138..1929)
                     /gene="kdgR"
                     /locus_tag="ACHJ7J_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO5000214.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2106..3479
                     /gene="yebQ"
                     /locus_tag="ACHJ7J_001978"
     CDS             2106..3479
                     /gene="yebQ"
                     /locus_tag="ACHJ7J_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5000215.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3528..4409)
                     /gene="htpX"
                     /locus_tag="ACHJ7J_001979"
     CDS             complement(3528..4409)
                     /gene="htpX"
                     /locus_tag="ACHJ7J_001979"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO5000216.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4601..6649)
                     /gene="prc"
                     /locus_tag="ACHJ7J_001980"
     CDS             complement(4601..6649)
                     /gene="prc"
                     /locus_tag="ACHJ7J_001980"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO5000217.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6669..7367)
                     /gene="proQ"
                     /locus_tag="ACHJ7J_001981"
     CDS             complement(6669..7367)
                     /gene="proQ"
                     /locus_tag="ACHJ7J_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO5000218.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7464..7961)
                     /gene="msrC"
                     /locus_tag="ACHJ7J_001982"
     CDS             complement(7464..7961)
                     /gene="msrC"
                     /locus_tag="ACHJ7J_001982"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO5000219.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8091..9374
                     /gene="yebS"
                     /locus_tag="ACHJ7J_001983"
     CDS             8091..9374
                     /gene="yebS"
                     /locus_tag="ACHJ7J_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO5000220.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9343..11976
                     /gene="yebT"
                     /locus_tag="ACHJ7J_001984"
     CDS             9343..11976
                     /gene="yebT"
                     /locus_tag="ACHJ7J_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO5000221.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12056..13495
                     /gene="rsmF"
                     /locus_tag="ACHJ7J_001985"
     CDS             12056..13495
                     /gene="rsmF"
                     /locus_tag="ACHJ7J_001985"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO5000222.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGE
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPPLPTPKYKVGNFPFSRVKD
                     REAAQIRQAAASVGLNWDENLRPWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            13613..13849
                     /gene="yebV"
                     /locus_tag="ACHJ7J_001986"
     CDS             13613..13849
                     /gene="yebV"
                     /locus_tag="ACHJ7J_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO5000223.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13954..14145
                     /gene="yebW"
                     /locus_tag="ACHJ7J_001987"
     CDS             13954..14145
                     /gene="yebW"
                     /locus_tag="ACHJ7J_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO5000224.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14146..14802)
                     /gene="pphA"
                     /locus_tag="ACHJ7J_001988"
     CDS             complement(14146..14802)
                     /gene="pphA"
                     /locus_tag="ACHJ7J_001988"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5000225.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(15199..15540)
                     /gene="yebY"
                     /locus_tag="ACHJ7J_001989"
     CDS             complement(15199..15540)
                     /gene="yebY"
                     /locus_tag="ACHJ7J_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO5000226.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15553..16425)
                     /gene="copD"
                     /locus_tag="ACHJ7J_001990"
     CDS             complement(15553..16425)
                     /gene="copD"
                     /locus_tag="ACHJ7J_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO5000227.1"
                     /translation="MLAFTWVALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16429..16803)
                     /gene="yobA"
                     /locus_tag="ACHJ7J_001991"
     CDS             complement(16429..16803)
                     /gene="yobA"
                     /locus_tag="ACHJ7J_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO5000228.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16942..17172
                     /gene="holE"
                     /locus_tag="ACHJ7J_001992"
     CDS             16942..17172
                     /gene="holE"
                     /locus_tag="ACHJ7J_001992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO5000229.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17274..17930
                     /gene="yobB"
                     /locus_tag="ACHJ7J_001993"
     CDS             17274..17930
                     /gene="yobB"
                     /locus_tag="ACHJ7J_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO5000230.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPACSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17954..18616
                     /gene="exoX"
                     /locus_tag="ACHJ7J_001994"
     CDS             17954..18616
                     /gene="exoX"
                     /locus_tag="ACHJ7J_001994"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO5000231.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18613..20673)
                     /gene="ptrB"
                     /locus_tag="ACHJ7J_001995"
     CDS             complement(18613..20673)
                     /gene="ptrB"
                     /locus_tag="ACHJ7J_001995"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO5000232.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20882..21541)
                     /gene="yebE"
                     /locus_tag="ACHJ7J_001996"
     CDS             complement(20882..21541)
                     /gene="yebE"
                     /locus_tag="ACHJ7J_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5000233.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERATIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21867..22223)
                     /gene="yebF"
                     /locus_tag="ACHJ7J_001997"
     CDS             complement(21867..22223)
                     /gene="yebF"
                     /locus_tag="ACHJ7J_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO5000234.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22290..22580)
                     /gene="yebG"
                     /locus_tag="ACHJ7J_001998"
     CDS             complement(22290..22580)
                     /gene="yebG"
                     /locus_tag="ACHJ7J_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO5000235.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22714..23892
                     /gene="purT"
                     /locus_tag="ACHJ7J_001999"
     CDS             22714..23892
                     /gene="purT"
                     /locus_tag="ACHJ7J_001999"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO5000236.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23948..24589)
                     /gene="kdgA"
                     /locus_tag="ACHJ7J_002000"
                     /gene_synonym="eda"
     CDS             complement(23948..24589)
                     /gene="kdgA"
                     /locus_tag="ACHJ7J_002000"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5000237.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24626..26437)
                     /gene="edd"
                     /locus_tag="ACHJ7J_002001"
     CDS             complement(24626..26437)
                     /gene="edd"
                     /locus_tag="ACHJ7J_002001"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO5000238.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26672..28147)
                     /gene="zwf"
                     /locus_tag="ACHJ7J_002002"
     CDS             complement(26672..28147)
                     /gene="zwf"
                     /locus_tag="ACHJ7J_002002"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO5000239.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28485..29354
                     /gene="hexR"
                     /locus_tag="ACHJ7J_002003"
                     /gene_synonym="yebK"
     CDS             28485..29354
                     /gene="hexR"
                     /locus_tag="ACHJ7J_002003"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO5000240.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29482..30924
                     /gene="pyk"
                     /locus_tag="ACHJ7J_002004"
     CDS             29482..30924
                     /gene="pyk"
                     /locus_tag="ACHJ7J_002004"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO5000241.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31055..32026)
                     /gene="lpxM"
                     /locus_tag="ACHJ7J_002005"
                     /gene_synonym="msbB"
     CDS             complement(31055..32026)
                     /gene="lpxM"
                     /locus_tag="ACHJ7J_002005"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5000242.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILAWLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32146..33468)
                     /gene="mepM"
                     /locus_tag="ACHJ7J_002006"
     CDS             complement(32146..33468)
                     /gene="mepM"
                     /locus_tag="ACHJ7J_002006"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO5000243.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33484..34416)
                     /gene="znuA"
                     /locus_tag="ACHJ7J_002007"
     CDS             complement(33484..34416)
                     /gene="znuA"
                     /locus_tag="ACHJ7J_002007"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO5000244.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34495..35250
                     /gene="znuC"
                     /locus_tag="ACHJ7J_002008"
     CDS             34495..35250
                     /gene="znuC"
                     /locus_tag="ACHJ7J_002008"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO5000245.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35247..36032
                     /gene="znuB"
                     /locus_tag="ACHJ7J_002009"
     CDS             35247..36032
                     /gene="znuB"
                     /locus_tag="ACHJ7J_002009"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO5000246.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(36246..37256)
                     /gene="ruvB"
                     /locus_tag="ACHJ7J_002010"
     CDS             complement(36246..37256)
                     /gene="ruvB"
                     /locus_tag="ACHJ7J_002010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO5000247.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37265..37876)
                     /gene="ruvA"
                     /locus_tag="ACHJ7J_002011"
     CDS             complement(37265..37876)
                     /gene="ruvA"
                     /locus_tag="ACHJ7J_002011"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO5000248.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9088 a         9813 c         9381 g         9708 t
ORIGIN      
        1 aatttgaagc agttgcacca tgacagtgcg tataaccagg atgaattgag taacccttca
       61 acgacggggt tactcaagat tgaggaagga ttatcgttcg gcgaccaggc gaataatatc
      121 ttcgtcttca accgattttt gactctcaat ggtcttttta tctgctttct ctggttcgtt
      181 tgcttcgcac aaacgacgta acagtgcatt ttgacgtttt tgctgatcca gcaacgcctc
      241 aagcagttca atctgttcgt tagtccgaga actggcacga ttaataaaaa accacaagat
      301 gagcccgata agaagaacca ccaccgatac aaccaaagac gcaatgttca tcacgcctga
      361 atttacaact tcgttcattt caccacctct gagtagaggc gctattctac cactgctgga
      421 gaggaagaaa atctagtgct gaaaaaatga tatcaccacg ggataaactg gttaatggca
      481 caaattccgc tgaaaaattg tacatcctga tcgcacatca tgttgaatac ctgcgcccaa
      541 agcagcaagc aagccaacac cacgacaacc aaaacgaccc atcgaaactt tttcactcca
      601 atctccgttt cacctacctt atgtcattaa actaccatgc gttagttaaa cagcgcctaa
      661 ctatgtcgat attcgtgctt tttcggaatg agtcgcttgc ttcagcgcat attgccgcta
      721 cgattaagcg aacataaaaa gagaagaggt tgtaatgcga ttcatcattc gcacagttat
      781 gctgatagca ctggtatgga taggattatt actcagcgga tatggcgttt tgattggaag
      841 caaagagaat gccgcaggat taggtttgca atgtacctat ctgactgcca gaggcaccag
      901 cacggtacaa tacttgcaca ctaaaagtgg ttttttgggg ataacagatt gcccgctgtt
      961 acgcaaaagc aatatcgtgg ttgataatgg ttaagggcaa taaataacca gattctcact
     1021 cctgagtaaa atactcttat agcaagcgcg tttaatataa cgccaccttc aactttattt
     1081 ttctcatgct aaataaagaa cctgtctgtt tgatattccg acaggttctt actctcatca
     1141 gaacggatag tcgtgataac ccatttgggc agaaattttg cgcgctgcgg tatgcaacat
     1201 tgcgacatat tcctgtaaac gctcttcaga gaaacgcaac gtcgggaagg agatgctcaa
     1261 accggcaatg accacgccaa agcgatcaaa taccggtacc gcaatgcatc gcagcccttc
     1321 ttcctgctct tcgttatctt cgccataccc ctgctcgcgc acctggtcca gaacgggtaa
     1381 taacgcttct gtactggtga tggtccgctc ggtactgcgt ttatactcca cgccctcaag
     1441 aatttgcttc acttcatcgc gatcgcgcca tgccagcagt accttaccaa tcgcggtgct
     1501 gtacagcgga ttacgacgcc caatccgtga atacatgcgc aaattgtaca tagagtcaat
     1561 tttgtgaatg taaacaatac tgtcttcgtc cagtgcgccg aggtggatag tttctttggt
     1621 caggcgggag atctcacgca tctggatatc tgcgctacga attaaatcga cgttttgtaa
     1681 cgcgcgagcg cccagttcaa acaatttcag ggtcagggaa tatttctccg actccccttc
     1741 ctgcgccaca taacctaagg ttttcatggt ctgtaaaaag cgataaacgg tgctttttga
     1801 catcatgacg cgctgtgaca gctcggttat ccctatttcg cgctcttcac ccagcgcctg
     1861 caaaatgcca aaaactttta gcacggaaga tacagaatca ggctgtttat ccagatctgc
     1921 gttagccatt tatcacctca ttgcgagtgt tttataaaaa tcagaactgt tttttattat
     1981 aatttcgcac ctgggtggtc gcaatccatc ttttgccggt tagttacaat tctgcgacat
     2041 ccaccgtgaa tatcagtgct agaatcatac ccctgttgat tattcaccaa agatataaaa
     2101 ttcctatgcc aaaagttcag gccgacggcc tgccgttgcc ccagcgatac ggtgcgatat
     2161 taaccattgt gattggtatt tcgatggctg tccttgacgg cgcaatcgcc aacgtcgcct
     2221 tgccaacgat cgccacggac cttcatgcca cgccggccag ttccatctgg gtagtgaacg
     2281 cctatcaaat cgccattgtc atctccctgc tctcgttttc gtttctgggc gatatgtttg
     2341 gctatcgacg tatttataaa tgcggtctgg tcgtttttct gttgtcttca ctgttctgcg
     2401 ccctttctga ttcgctgcaa atgctcaccc ttgcgcgtgt catacaaggt ttcggcggcg
     2461 cagcgttgat gagcgtaaat accgcactta tccgcctgat ctatccacaa cgttttctgg
     2521 gtagagggat gggcataaac tcgtttattg ttgccgtttc ttctgctgcc gggccgacaa
     2581 ttgctgcagc aatcctctcc atcgcatcct ggaaatggtt atttttaatc aacgtaccgt
     2641 taggtattat cgccctgctt ctggcgatgc gttttctgcc acccaatggt tctcgcgcca
     2701 gtaaaccccg tttcgacctg cccagcgccg tgatgaacgc gttaaccttc ggcctgctta
     2761 tcactgcgtt gagtggtttc gctcaggggc aatcgctgac gttaattgct gcggaactgg
     2821 tggtaatggt tgtcgttggt attttcttta ttcgccgcca gctttctctt ccagtaccgc
     2881 tgctaccggt ggatttactg cgtatcccgc tgttttcact ttctatttgc acatctgttt
     2941 gctctttctg cgcacaaatg ctggcaatgg tttccctgcc cttttacctg caaaccgtgc
     3001 tcgggcgtag tgaagtcgaa acaggtttac ttctgacacc gtggccgtta gcaacgatgg
     3061 tgatggctcc gttggcaggc tatttgattg aacgcgtaca tgcaggattg ctgggtgctt
     3121 taggattatt catcatggct gcggggcttt tttccctggt tctgctgccc gcgtcacctg
     3181 cggatatcaa tattatctgg ccgatgatct tatgtggcgc tggatttggc ttgttccagt
     3241 cacccaataa ccacaccatt attacctccg cgcctcgcga acgtagcggt ggagccagtg
     3301 gcatgttagg aacggctcgt ctactgggtc agagtagcgg cgcggcgctg gtggcgctga
     3361 tgctaaatca gtttggagat aatggtacac acgtctcgct gatggctgcg gctattctgg
     3421 cagtgattgc tgcctgtgtc agtggtttac gtatcactca gccacgatcc agggcataaa
     3481 aaaagcgcgt cgatcaggac gcgcttttta gtgtttactt cataatatta cttcaggtat
     3541 tcacccgtac gcagagcttc aatacgttta tccagcggcg ggtgagtcat aaacaactca
     3601 ctgagcgatt tcgacttacc gttaatgcag aaagccatca tgctggttgc ttcttgcggt
     3661 tcatagctgg ttttcaggcg ctgcagcgcg gcaatcattt tctcgcgacc aaccagtttt
     3721 gccgaaccgg catcagcatg gaattcacga tgacgcgaga accacatggt gataatgctc
     3781 gccagaatac caaacaccag ttccagaacc gttgcaaccg caaagtagat cagcgggttg
     3841 ccgttgctct cttcaccttc atcacgattt ccgcccataa aacccgcggc aagctgcgcc
     3901 aggatacggg aaataaagat aacgaaggtg ttcaccacgc cctgaatcag cgtcatggtg
     3961 accatatcac cattggcgat gtggctgatt tcgtgagcaa ttaccgcctc ggcttcatcc
     4021 gggctcatgt tctgcagcaa accggtgctg acagcaacca gagaggcatc acggcgcgca
     4081 ccggttgcaa aagcgttgat gtccggcgca tggtagatag ccacttgcgg catagcgatc
     4141 cccgcctgac gagcctgggt tgctacagta ttgaccagcc aacgttccct ttcgttacgc
     4201 ggttgctcga tcacttcccc gccaacagat cgtaatgcca tccatttgga catcagaagc
     4261 gaaacgaagg aaccaccaaa accgaacagc aaggccatga tcatcagccc ctgaacgctg
     4321 ctcgactgta tccctgtcag gctcagtacc agcccgaaaa cgaccattac ggccaggttc
     4381 gttagcagga agagcgcgat tcgcatcata attttctttt tacctcagtt taacaaaacg
     4441 caatatgcga tacccacatc gtatgggtta cgcgactatt ttcaagtctg gatagtgcgt
     4501 aagtcaccag aaagacacaa ctttacattt tgtagcatct gatttacggc atcttgccgc
     4561 tgttaaaaaa tcaggcacaa tttcttgtgc ctgattgata ttacttgacg ggtgcgggtt
     4621 gttccgcggg tctggctttt tcaagcttcg ccagatcgag tgcgatattc accgtctcat
     4681 ccagataagg atccggctcc tggtaatctt tcggtagatc atccagtttc ttcaactccg
     4741 gtttaccttc gcgtttaaag cgttcgttca aacgcgccag acgcgtcgca tcatcttcat
     4801 tattctcttt ctcacgcaca gcgtaattca gagaaacgat attgcgcttg tccttcatag
     4861 cgttgaagcg cgcgatatcc ttcatgatgt tctggaactc aggatctttc gcgatacgcg
     4921 cattatgttc cttcagtagc tccggttcaa aggccgttaa atctcctgat ttcacataag
     4981 tcgcggcatc aatgctatcc cacggcagcg cgttatcttc gaatttctca cccgtttccg
     5041 tttcttcatt acccgtcggc atgatgatgt ctggcgttac gcctttacgt tgcgtactgc
     5101 cgccgttaac gcgatagaat ttctggatcg tgtactgcac agaacccagc gctggccatt
     5161 caggacgtaa catctgatcg tagatacggt tcaatgaacg gtattgctga acggtgcctt
     5221 taccaaacgt cggttcaccc acaaccagcg cacgaccgta gtcctgcatt gccgcggcaa
     5281 agatttctga agccgaagca ctgaagcggt caaccagtac caccagcggg cctttataga
     5341 aaacctgtcc gtcggtatcg ctgtcttcac gaactttacc gttgttatca cggacctgaa
     5401 caataggacc cgcaggaata aacagacctg agagcgatac cgcttcggtc aacgccccac
     5461 cgccattgct acgcaggtcg atgatcacac tgctgacatt ctgtttttcc agtttctgca
     5521 gttgcacttt gacatcgtct gtcaaaccca catagaagcc aggaatatcc agcacgccga
     5581 ctttctcttt accgacggtc ttcaccgaca ttttaaccgc gcggtcttcg agacgaatac
     5641 gttcacgggt caatgttaca gtgcgggtct tggtcccttt accagcaggt aaaatttcca
     5701 gacgaacttt actgcccttc ggccctttaa ttaaggcaac cacatcatca agacgccagc
     5761 caatcacgtc aaccatcggc ttgcctgttt gaccaacacc gacaattttg tcaccaacgc
     5821 tgatagcttt actcttcgct gccggaccac ctgccaccat cgaattgata acggtgtagt
     5881 catcatccat ttgcagcact gcgccaatac cttccagcga caaactcatt tcagtgttga
     5941 actgttcggt attacgcggg gaaagatagt tggtatgcgg gtcgatttca cgcgcaaacg
     6001 ccgtcattgc cagcgagaaa acatcttcgc tgttggtttg cgccagacga cgaatggcaa
     6061 atttgtagcg gcgagtcagg gtttcacgaa tttctttatc cgtttttcct gccagcttca
     6121 ggcttaactc gtcgaatttg actttactgt cccacagcgc gttcaactca gcctcgtttt
     6181 tcggccaggg cgctttgctg cggtcaaggt tataagtgtc gttgccggtg aaatccatcg
     6241 gcttttctag taccgacaaa gcgtactggt aacgctcaaa acggcgcttt tgcgccagat
     6301 tgtagagatc gtagaaaacg tcgagtttgc ctgaacgcag ttcatcgcct aactcggttt
     6361 tctttttcgc gaactgttca acatcgcttg ccaacagcac gttgtggctg taatcgagca
     6421 gattcaggta gcggtcaaag attttggccg aaaatgcctg atcgaggtcg aactggcgat
     6481 aatgagaacg ggtgaagcgc gacgttacgc gctcactcac cgtcgcatgc tgcgtctctt
     6541 cctttaatac cggaatttga tcagcacgcg tgatatcttc tacagcgaag gtctggcctg
     6601 ctattgcaag caggccagct aacgcggtaa gcctaaaaaa catgttcatg cctggcccgg
     6661 cctccgtttc agaacaccag gtgttctgcg cgcacaatca aagacatacc cgaattcagc
     6721 tggacgcgga cgccgtcttt ggtgatttct aatacggtgg catccatcgc gttttgaccc
     6781 gctttcacct tcagggcttg tccgacagtc agagctgaaa tgtcagaaac tggggtgtgc
     6841 tgttcttcgc gaggtgcttt tgctgttttt ggcgctttct ctaccggctt ttgcgcacga
     6901 ggtttacgtt cagcgccttc tttgcggcgt ggcgtggtcg gacgtggctt gcgttcgcga
     6961 cgcggtgcgt cttctttctc accagcagct gcggcagctt cgcgtttttt cgcttgctgt
     7021 tcagcacgct gtgcctgaac acgcgctttc gcttcttcaa gctgcttgcg agcatgttct
     7081 acatgttgct cgtccagctc accgcatggg ttgccgtcaa gatcgacacg cgttgcgccg
     7141 ggtttaacac cgtaaaggta acgccagctc gaagtgtaga gacgtaaagc ggatcgtaat
     7201 tgcgttttgc tcaggttcat ttccccggca acacgatcga ccaaatcctg aaaaataccg
     7261 attttcagcg gacgcgcttc accttccgca ctgaaacagt ggggaaaacg ttcggccaga
     7321 aacgcgatta cttctttact gctattcaac ttaggttgat tttccatgaa atttcctgat
     7381 tacaacggac gtagccaaca agcgcaggca tgaacaggcg tcattataat gacgctatca
     7441 gtaaatgcta cgttatccgt tgattatcct gcgacgctcg caaagaattt tttgtaatcc
     7501 gtcgttgcaa gcaccttttc aagctgtgcc acaagctgac gtaagccttg ctcgtcctcg
     7561 tctgtaaagc gaccgaagac ggtactatcg atgtcgagaa caccaataat ctgatttttc
     7621 accaccagcg gcagaacaat ttcagaatta ctcgccgcat cacaggcaat atgcccgtca
     7681 aacgcatgaa catcctcgat acgctgcact tgattgcggg caactgcagt gccgcacacg
     7741 ccacgcccga cgggtatccg gacacaggca attttgccct gaaatggtcc gagcaccagt
     7801 gtatcgtcct caagcaaata aaaacctgcc cagtttacgt cagtgagacg ctcatataac
     7861 aacgcactgg tgttcgcaag cgttgccaga aaactggttt ctcccgccat cagcgcgtta
     7921 aagtcgcgat ttaaatccgc gtaaaattct gttttgttca ttatataatc acttggttgt
     7981 cttacctgga actgccagcc tattaaaata agcattaaat gcgttaatgc tcaagatcat
     8041 tcccatcatg ggttaagatt aatgttaatt cttattacat ttggcacgtc atggctctta
     8101 acacaccaca aattacgccg acaaaaaaga taacagtgag ggcaatcggc gaggaactgc
     8161 cgcgtggtga ttaccaacgt tgcccgcaat gtgacatgct gtttagcctg cccgagataa
     8221 attctcatca aagtgcctat tgtccgcgct gtcaggcaaa aattcgtgac gggcgcgact
     8281 ggtcgctaac gcgcctggcg gcaatggcct tcaccatgct gttgctgatg ccgtttgcct
     8341 ggggcgaacc gctgttgcat atctggctgt taggcattcg tattgacgcc aacgttatgc
     8401 aaggcatctg gcaaatgacc aaacagggcg atgcgataac ggggtcgatg gtctttttct
     8461 gcgttattgg tgccccgctc attctggtga cctccatagc ttatttatgg tttggtaacc
     8521 gactgggaat gaatctacgc ccggtactgc tgatgcttga gcgacttaaa gagtgggtaa
     8581 tgctcgatat ctacctggtc ggcattggcg ttgcttctat aaaggtacag gattatgccc
     8641 atatccaggc gggtgtcggc ttgttctctt ttgtggcgtt ggtgatttta acgacggtga
     8701 cgttgtcaca tcttaatgtc gaggagctgt gggagcgatt ttatccgcag cgccccgcta
     8761 cgcgtaggga cgagaaactt cgtgtctgtc ttgggtgcca ttttaccggc tatcctgatc
     8821 agcgtggtcg ctgcccgcgt tgccatatcc cgctacgcct gcgtcgccgt catagtttgc
     8881 aaaaatgctg ggcggcgctg ttagcgtcaa tcgttttgtt gttacctgcc aacctgttgc
     8941 ctatttctat catttatctg aatggtggcc ggcaggaaga tacgattctt tccgggatta
     9001 tgtcgctggc aagtagcaac attgccgttg cgggaatcgt gtttatcgcc agtattctgg
     9061 taccgtttac taaagtgatc gtcatgttca ctttactgtt gagcattcat tttaaatgcc
     9121 agcaaggttt acgcacacgc attctgttac tgcgaatggt gacctggatt ggtcgctggt
     9181 cgatgctcga cctgtttgtc atatctttaa ccatgtcgct gattaatcgc gatcagatcc
     9241 tcgcttttac tatgggaccg gctgcgtttt atttcggcgc agcggtaatt ttgactattc
     9301 ttgctgtgga atggctggac agccgcttac tttgggatgc acatgagtca ggaaacgccc
     9361 gcttcgacga ctgaagcgca gattaaaaat aaacgccgta tctcaccttt ctggctgctg
     9421 ccgttcatcg cgctaatgat tgccggttgg ctgatttggg acagttatca ggaccggggt
     9481 aataccgtca ccatcgactt tatgtcggcg gatggtattg ttccaggccg tacgcctgtt
     9541 cgttatcagg gcgttgaagt cggaacagtg caggatatca gcctcagcga cgatcttcgt
     9601 aagattgaag tcaaggtcag catcaagtcc gatatgaaag atgcgctgcg cgaagagact
     9661 cagttctggc tggtgacgcc aaaagcatcg ttggcaggtg tctccgggct ggacgccctc
     9721 gtcggtggga actatatcgg catgatgccg ggtaaaggta aagagcagga tcactttgtc
     9781 gcgctcgata cccaaccgaa atatcggctg gacaatggcg atctgatgat ccacctgcaa
     9841 gctcccgatc tcggttcgct gaacagcggt tcattggtct atttccgcaa gatcccggtg
     9901 ggtaaagtct acgactatgc catcaatccc aataaacaag gcgtggtgat tgatgtcctg
     9961 atcgagcggc gttttaccga tctggtgaaa aaaggtagcc gtttctggaa cgtttccggc
    10021 gttgatgcca acgtcagtat cagtggcgcg aaggtgaaac tggaaagcct ggcggcactg
    10081 gttaacggtg cgattgcctt cgattcacct gaagagtcga aacctgccga ggcggaagat
    10141 acctttggtc tgtatgaaga tctggcccac agccagcgtg gcgtaataat aaaactggaa
    10201 ctgccgagtg gggcaggatt aaccgccgac tcgacgccgt taatgtatca ggggctggaa
    10261 gtcggacagc tgactaaact ggatttaaat cctggtggta aagtcaccgg agaaatgacc
    10321 gttgatccca gcgtcgttac gctgttacgg gaaaataccc gcatcgaatt acgcaacccg
    10381 aaattatccc ttagcgatgc caatctcagc gccctgctga ccggaaaaac ctttgagttg
    10441 gtgcctggcg atggcgagcc acgcaaagag ttcgttgttg tgccaggcga aaaagcactg
    10501 ctgcatgaac ctgatgttct gacactgacc ctgacagcac cggaaagtta cggtattgat
    10561 gctggtcagc cgctcattct tcacggcgtg caggtaggcc aggttattga tcgtaaactc
    10621 accagcaaag gcgttacctt taccgtcgcc attgagcctc agcatcgcga actggtaaaa
    10681 ggcgatagca aatttgtcgt caacagccgt gtcgacgtga aggtggggct ggatggcgtt
    10741 gagtttctcg gtgccagcgc ctcagaatgg attaacggcg ggatacgtat tctgccgggc
    10801 gataaaggcg agatgaaagc cagctatcca ctgtatgcca atctggaaaa agcgctggag
    10861 aacagcctta gcgatttacc caccacaacc gtgagtttga gtgcagagac gctgccggat
    10921 gtgcaggcag gatcggtagt gctgtaccgt aaatttgaag ttggtgaagt gattaccgtg
    10981 cgtccgcgag ctaacgcgtt tgatatcgat ctgcatatta agccggagta tcgcaacctt
    11041 ctgaccagca atagcgtgtt ctgggcagaa ggcggggcga aagttcagct gaatggtagt
    11101 ggcctgaccg tacaggcatc cccgctctcc agagcattaa agggagccat tagcttcgat
    11161 aacctcagcg gtgccagcgc cagtcagcgt aaaggcgata agcgtattct gtatgcttcc
    11221 gaaacagcgg cccgtgcggt tggcgggcag attacgcttc acgctttcga tgccggaaaa
    11281 ctggcggtcg ggatgccaat tcgctatctc ggtattgata tcgggcaaat ccagacgctg
    11341 gatctgatta ccgcgcgtaa tgaagtacag gcaaaggcgg tgctctatcc ggaatatgtc
    11401 cagacttttg ctcgcggagg tacgcgcttc tcagtggtca caccgcaaat ttcggcagct
    11461 ggcgttgagc atctagatac tatcctccag ccgtatatca acgtcgaacc aggccggggc
    11521 aatcctcgcc gcgactttga attacaagag gccaccatta ctgattcgcg ttacctggat
    11581 ggcttaagca ttattgttga agcgccggaa gccggttcgt taggcatcgg tacgcctgtg
    11641 ctgttccgtg gtctggaagt cggtacggtt accggaatga cgctggggac attgtcagat
    11701 cgcgtgatga ttgcgatgcg catcagtaaa cgctatcaac acctggtgcg taacaattcc
    11761 gtcttctggt tggcatcggg ttacagtctg gactttggtc tgacgggcgg cgtagtgaaa
    11821 accggcacct ttaaccagtt tatccgtggc ggcatcgcct tcgccacgcc tccgggtacg
    11881 ccactggcac cgaaagccca ggaaggcaag cacttcctgt tgcaggaaag tgaaccgaaa
    11941 gagtggcgtg aatggggtac tgcgcttccc aaataatgcc cactgctccg gcgtgcctgc
    12001 gccggagcgt ttatgctaaa ctgcgcgcct gtttttttgc cagtggtata tgctcgtggc
    12061 ccaacacacc gtttatttcc cggacgcctt tctgacgcaa atgcgcgaag cgatgccttc
    12121 gacgctctct tttgatgatt ttcttgccgc ctgtcagcgc ccgttgcgcc gcagcattcg
    12181 cgttaatacg ctgaaaatct ccgttgctga tttcctgcaa ttaaccgctc cttatggctg
    12241 gacgcttacg ccaattccgt ggtgtgaaga aggtttctgg attgaacgcg acaatgaaga
    12301 tgcattgcca ttgggtagta ccgccgagca tttaagtggc ctgttttata ttcaggaagc
    12361 cagttcaatg ttgcccgtcg ccgccttgtt tgctgacggt aatgcaccac agcgggtgat
    12421 ggatgtcgct gctgcgccag gctccaaaac gacgcaaatt gccgcgcgga tgaataacga
    12481 aggggcaatc cttgccaatg agttttccgc cagtcgggta aaagtgttac atgccaatat
    12541 cagccgctgt ggcatcagta atgttgcgct cacacatttt gatggccgcg tgtttggtgc
    12601 ggcagtgcca gaaatgttcg atgccatttt gctggacgct ccctgctctg gcgaaggcgt
    12661 ggtgcgtaaa gatcccgatg cgctaaaaaa ctggtcacca gaaagcaatc aggaaatcgc
    12721 agctacacaa cgggagctta tcgacagcgc ctttcatgca ttacgtcctg gtggtacgct
    12781 ggtttactcg acctgtacct taaacaggga agaaaacgaa gccgtttgcc tgtggctgaa
    12841 agagacttac cctgacgcag tagagttttt accacttggc gagctcttcc ctggtgcaaa
    12901 caaagcgctg accgaagaag gctttttgca tgttttccca caaatttacg actgcgaagg
    12961 cttcttcgtt gctcgtctac gtaaaactca ggctattccc cccttaccca ccccaaaata
    13021 caaagtgggc aatttcccgt tcagccgggt gaaagatcgc gaagccgcac aaattcgtca
    13081 ggcggctgca agtgttggct taaactggga tgaaaacctg cgcccctggc agcgtgacaa
    13141 agaactgtgg ttgttcccgg tgggcattga agccctgatc ggtaaagtcc gattttctcg
    13201 cctggggatt aaacttgccg agacgcataa caaaggttat cgctggcagc atgaagcggt
    13261 tattgccctt gcctcccccg acaacgagaa cgcttttgaa ctgacaccgc aagaagcgga
    13321 ggagtggtat cgcgggcgcg atgtttaccc gcaagccgcg ccagtggcgg atgacgtgtt
    13381 ggttactttc cagcatcagc cgattggttt agccaaacgg attggttcgc gactgaaaaa
    13441 cagctacccg cgtgaactgg tgcgggacgg gaaacttttt accagtaacg catgacagcg
    13501 cacaaaaaaa gcgcactttt tgactggcac attcggcggc ctcaactagg ctgaaaaatg
    13561 gtgcgatcgg actggtcgta ccacaatcgg cagctaaatg gagagcacta agatgaaaac
    13621 cagtgtgcgc ataggcgctt ttgaaatcga cgacggcgaa ttacacggtg aatcgccggg
    13681 tgatcgaacg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc aactggatgc
    13741 ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg tcctttaccg
    13801 tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat ccaaaaagaa
    13861 cccgtcggca tggcgggtta tttgtcctgg ttattccccc gttgtaaaat ctctcctaaa
    13921 cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact ttttgtttgc
    13981 tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga acagcagtgt
    14041 ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat tgaggatttt
    14101 atagatggtt tctggcgacc agcacaggag tatggtgatt tttaattatt gcaattgcac
    14161 gaaagtcagt tcgcccccaa agacagcgcc ggtatcaata taatgcaggt tgccaatatc
    14221 cacgcgatgg cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg taattccctg
    14281 ccctttttgg cgttcactta atcgcgagcg gctccacaag acctgatgca aatcaacgtc
    14341 cttttgccat tcataaacat catctggata atcggcatga gcaataacat gtttgccggt
    14401 gcggctgtgt acttcaagaa taaagggcaa atgctgacat ttttccagcg ccgttttcgc
    14461 ttgtttctgt tgattatctg ccagcgcaat aaaccagtcg ccgccattca tcaaccacaa
    14521 agacatctgc tgggatgcca gcgcatccat cgccatctgt tcatgattgc ctcttaccgc
    14581 acgaacccaa tgttgttcca gtaactgcag acaacgtaaa ctttgcggcc cacgatcgat
    14641 aacgtctccc actgagataa gtaaatctcg ccacggatca aaacgacaat gccataattt
    14701 gcggcgcaac tgctcaagac aaccgtgtat atcgccagaa agccagatat gtcgccattg
    14761 atgacccgca attctctgat aaacgggcgc aggctgtttc atcaatattt tcctcccgcg
    14821 ctaaagatca cataatctta acaagaatgt taaaaaacgc tggactcaga cagtagagtg
    14881 tgtgttatgg ttgactataa agtcagcgaa ggaaatgctt ctggctttta acagataaaa
    14941 agagaccgaa cacgattcct gtattcggtc cagggaaatg gctcttggga gagagccgtg
    15001 cgctaaaagt tggcattaat gcaggcttag ttgccttgcc ctttaagaat agatgacgac
    15061 gccaggtttt ccagtttgcg tgcaaaatgg tcaataaaaa gcgcggtggt catcagctta
    15121 aatgttaaaa accgcccgtt ctggtgaaag aactgaggcg gtttttttat tggaaatcaa
    15181 aaagactatt ttaggtaatt aacagagttt ttcagcccgt tctataaacg gtgccagact
    15241 cattttttcg ccgggattgt tgggatcatc aatctgaatc accgaaatgg gttgggcttt
    15301 agtcttccca ctggcaactt ccttctgtgc gatatcgttt aaaggatact gcacgagggt
    15361 acttgggtta atgacataca aagcattacc cggtcggcaa gtcagcatca cctcttcgcg
    15421 attaaacgcc catttgtctt tacccacttc aaaacggctg acggtaatca cctgcggtgc
    15481 agccagcgcc gctgcagaac tggtgagtaa cagaaacgcc agaatacttt ttttcatcat
    15541 tttcgtaatc catcaaaaag gttcgagagt cgcaagcagg ctgatgatca ccagcactac
    15601 cgcacctatc gcccattcga gtttagtcat ccacacaaac cagggtgccg cacgatcttc
    15661 atcctgcctc atgcgcggta caagaacata acgattagcc agcgcaatga ccaccataat
    15721 catcaccagg atggctttga gcaataagag ttggccccag taagtcgtaa gcgtgggggg
    15781 aaatccggta atcaacaatg cattaagcac gccgcttgcc agtacgccga tcaccgcaaa
    15841 atgcccgcac caggaaaaac gcatcagcgc ctgaatagcc tggtgtcgcc aacggccttt
    15901 gatgagttgc atacaccaga gcaccggcag taatccccca aaccacgccg ccgcacaaat
    15961 caggtgaatc gcgtgattag tctgatggat tttcgccgtt accccttcat tcagcgtcgc
    16021 atggccgact cccgccagca gaataaattg cgcggtagtg agcataaaca gcaaacgtgg
    16081 catattccgc ggttgcataa gggcgacgat caacgttacg agggcgagaa caatttgcca
    16141 taaccagacg ccaccaaact gcgtttgcaa aacagcctgc caaatgtttg gcgaaaatac
    16201 atctgtccat cccgttccca tcaacccgcc ctgaacggcg agcattgcag tggcactgat
    16261 caaactccag acggcagcgt gctgttgtaa acgcagaaaa cgcttcgcca gcaaacgacg
    16321 aatcgttaag ggggccagcc aggcaccgta cattgcgaag ccaaaaacca gcatcagcga
    16381 ggtgaaatgg ataaatcgca gcgcgaccca ggtaaacgcc agcatgattt atttcacgct
    16441 aaaggtgtag tgtcctttcg ttttatgccc gtccaccgaa acaacatgcc agtcaacggt
    16501 gtaggttccg ggtttcagcg aatcggccag tggaacaatc agttgctttt ggtcctgctc
    16561 atttcgcttc gccggtaatg ttttaatatt ttcgtttttt ggccccgtga tttttgcacc
    16621 actgaatcct gtttcaacac cttccgagaa gtttaaagtg attgcctgcg gtgcagctgt
    16681 cacttgcgcg tttgccgcag gatactgatg cgttaaatgc gcatgtgccc agacggaagg
    16741 tgtcaccaac gaagtggtca ggattgccag cgcgtagcga agggagcgtg cagttgaagc
    16801 catattatct attccttttt gtaataactt ttttacagag cataaccttg tctaatgtct
    16861 gagtcgagga tcatcaattc cggcttgcca tccaggctca ctcttagtaa cttttgcccg
    16921 cgaatgatga ggagattaag aatgctgaag aatctggcaa aactggatca aacagaaatg
    16981 gataaagtga atgtcgattt ggcggcggcc ggggtggcat ttaaagaacg ctacaatatg
    17041 ccggtgatcg ctgaagcggt tgaacgtgaa cagcctgaac atttgcgcag ctggtttcgc
    17101 gagcgtctta tcgcccaccg tttggcttcg gtcaatctgt cacgtttacc ttacgagccc
    17161 aaacttaaat aaaacttata cagagttaca ctttcttaca taacgcctgc taaattatga
    17221 gtattttcta aaccgcaccc ataatttgca gtcattttga aaaggaagtc attatgtcgt
    17281 tctggaaagt tgcagcagcg caatatgagc ctcgtaaaac ctcactcacc gaacaggtag
    17341 ctcatcatct ggagtttgtg agggccgccg ccaggcaaca atgtcagctg ttagtttttc
    17401 catcgctttc tttactgggg tgtgattatt cgcgacgtgc ccttcctgcc ccacccgatc
    17461 tctcactgtt agacccgctt tgttatgccg caacgacctg gcgaatgacc atcatagccg
    17521 gccttcccgt tgaatataac gatcgcttta ttcgtggaat tgcggtgttc gcaccgtggc
    17581 gaaaaacgcc ggggatctac catcagagtc atggtgcatg tctgggccgt cgttccagaa
    17641 ccatcacggt ggtcgatgaa caaccgcaag gtatggatat ggacccggcc tgttcactgt
    17701 ttacgacagg gcaatgtctg ggggaacccg acctactggc atctacccgc cgcttacaat
    17761 ttttttcaca tcagtactcc attgccgtgc tgatggcaaa tgcccgtggt aacagtgcac
    17821 tatgggatga acatggtcgt ctcatcgttc gcgccgatcg tggttcatta ttgttagtcg
    17881 gtcagcgttc atcacagggt tggcaaggcg atatcattcc attacgctag gctttttcgg
    17941 cctggagcat gccatgttgc gcattatcga tacagaaacc tgcggtttgc agggagggat
    18001 cgttgagatt gcctctgttg atgtcattga cggaaaaatc gtcaacccca tgagccacct
    18061 ggtgcgcccc gatcgtccta ttagtccaca agcgatggcg attcatcgca tcaccgaagc
    18121 catggtcgcc gataaaccgt ggattgaaga tgtgatccca cactattacg gtagtgaatg
    18181 gtatgtcgcg cacaacgcca gctttgaccg ccgcgtactg cctgagatgc ccggtgagtg
    18241 gatttgcact atgaaactgg cccgtcgttt gtggccaggg atcaagtaca gcaatatggc
    18301 gttatataaa acacggaagc tcaatgtaca gacgccgccg ggcctgcatc atcaccgcgc
    18361 gttgtatgac tgttatatca ccgccgcgtt gcttatcgat attatgaaca cctccggctg
    18421 gacggcagaa cagatggccg atatcaccgg acgtccgtcg ttgatgacga ccttcacctt
    18481 tggcaaatac cgtggcaaag cggtttccga cgttgccgaa cgcgatccgg gctatctgcg
    18541 ctggttattt aataacctgg acagcatgag cccggagctg cgtttaacac tgaaacatta
    18601 tctggaaaat acttaatccg caggcgtagc gggtaatgtt ccctgcgcca gcgcgaccag
    18661 aaaagcatat tccatcgcta cgccttcgta cgatttaaag cgaccagatt taccgccatg
    18721 gcctgagtcc atgtcggtac agagcaataa aagatggtca tcggttttca gctcgcgcaa
    18781 tttagcgacc cattttgccg gttcccaata ttgcacctga gaatcgtgca aaccggtcgt
    18841 taccagtaaa tgcggataag cctgtgcggt gacgttgtca tatgggctgt agcttttcat
    18901 gtactcgtaa tattgcggat cctgcggatt cccccactct tcaaactcac cagtggtaag
    18961 aggaattgat tcatcaagca tcgttgttac aacatcaaca aacggtacct gggcgataac
    19021 gccgtggaat aattccgggc gttgattaat tgcaacgccc atcaacatgc cccccgcact
    19081 cccgcccatc gcataacaaa gcgaaggaga gccatagccc agttttaaca atgcatcgca
    19141 ggcatcaaga taatcattaa acgtattttt cttcttcaga aatttaccgt cttcgtacca
    19201 ttgttgcccc agctcaccgc cgccgcgaac atggacaatg gcgtagacaa aaccacgatc
    19261 taacaaactc aagcggctaa aactgaaatc ggcatcaata cttgcgccgt aagaaccata
    19321 gccatacacc agcaacgggt tgtgcccttt gcgaaaatgt ttgcgatggt agaccaacga
    19381 aaccggaact tcgacgccat cacgggcgac tatccacagg tgttcactgc ggtaattcgc
    19441 cgcatcaaaa ccaggaactt ccgtttgttt taatacacga cgctcaccgg tatccatatc
    19501 cagttcaaac aaagtgtctg gtgtggtcat ggaagaataa ccataacgca atcgcgcggt
    19561 ttcaggttct ggattgtagg caatccaggt cacataggct ggatcatcaa aggcaatacc
    19621 aatgacttcc cgggtcttgc ggttaatttg gcgcaaactg gttaacccgc gctgacgctc
    19681 ttcaaccacc agccagtcgg taaacagcgt aaacccttcc agcatgatgt tttcgcgtgg
    19741 cggaattaac tcttcccact gttgctcatc acgcatacgg gtacggtata agccaaagtt
    19801 tttgccgtgg cggttggaac gcagataaaa acgatgctgg tagtgatcaa ggctgtattc
    19861 gtgatctttg cggcgcggca gaaaaacaaa cggctcggca tcggccattt ccgcgtccag
    19921 caggcgaact tcactggtgg tggcgctggc caaatgaatg actacatagt gcttcgaggt
    19981 cgttttatgc aggctgacgt aataggtatc gtctttttct tcgtaaatca gtttatcttg
    20041 cgatgctggc gtaccgatgg cgtgacgcca gacctgataa ggcagcagcg tcaccggatg
    20101 cttgcggaca tagtagaaag tccaggagtc atttgcccag acaaagctgg gttcaacgtt
    20161 atccagcagt tccgggtacc agttacctgt ttccagatta cgaaaacgaa tgccgtactg
    20221 gcgtcgggaa agaaaatctt ctgccagcgc cataatggtg ttatcgggcg taatcgccat
    20281 tccgcccatc gaataaaact cactatgagc cgcgcgcttg ttggcatcga gcaatatttc
    20341 ccactcgtcc cactcttcac tgaacgccga ttgacgctgg tagatagcat attcacagcc
    20401 tggttcataa atatgccgat agcggtagcc atttttgatg tagggcgcag aaacttctct
    20461 ttgcggaatg cggtcgatga tttcctttaa gatgcgatcc tgcaaggctt gttgtgaggc
    20521 catcacccga tgaccgtaac tattttcttg ttgcaggtag tccaggactt ccggctgaga
    20581 acgcgtatcg tcccgcagcc agtagtaatt atcgatgcgc gtatcgccat gaagcgtcat
    20641 ggcgtgggga atgcgggcgg cttttggtag catgttattg ttctttctgg ttgaaacatc
    20701 ttataagggt ggcaaaactc accggggatg caagcgaaac aggggagtca ttgcttagat
    20761 gatgacaggt aatggcgcgg atatcgaatg ttatgcagac agagaaaatc agcctgttca
    20821 aatggctgtg cggttctgga tagcccgaaa tagtcaactt caggctatcc agagagcgga
    20881 attattccgc caaagtgcgt ttttgctgtt cgagatcgcg ttcaatgcct tcacgaacat
    20941 cctgggggat tttcagcgcg tcacccagtg cattcaggta actgcgttcc ataaaatggt
    21001 caatatcaat agccgcgcag ctcaggaaat agatttccag cgcctcttct tcattgcgga
    21061 ccccggtagc caggcgttgt ggatccagcg gttgttcgat tgcctgctca atgagtaccc
    21121 gcccctgctc ttccacgccg gcttcacgca attgctggtc gattgtcgca cgttctttgg
    21181 catcaatatg accatcactt ttagcggcaa agactagcgc aaggatcaaa cgttccgtac
    21241 gctcatccag cggcgtactt tgcgcgccaa actgcggttc gtcctgatgc gccgcgcgaa
    21301 ttttatcttt gtatttattc cacagcaccg tacccgctac cgctccgccg ccaaccagca
    21361 acgcgttggt gccatatttc gtaagaagtt tacgtgctga tttatttgcg accagcagtc
    21421 cagccagtcc acctaatgcg cccggcacta aaagtttgcc caatccctga tccgcagacg
    21481 aggaggtaga agaactgctt tgcccaagca gggattgcag ttgatttaac cagttagcca
    21541 tatttgctcc tcaataacca tttttagtgc ctgtagcata gtggatgagg atgtcagaaa
    21601 atgtatatcg agacaaaaga tgcgcaaata agcggggcgg ttggccgtta ttcaaagaaa
    21661 ttcgcgccag gtaaggtgtt tactgtatat caggtgataa gggtatttta ggtgaaagta
    21721 gtttgacgtt ttgagtggca ttgtttgatg ctgattgccc gatgcgacgc tgttgcgtct
    21781 tctcaggcct acgattgtgg tgcgttttgg ggcggacaag gcactcgcgc ccatccgcca
    21841 acggtcaatc gaattatcag aacccattaa cgccgctgat attccgccat tcccgctttg
    21901 cagtccacgc tgacctggta atgaatatcg gcacttttac cacgcacggt tagcggtact
    21961 gaccatttat catctttacc ctgaatgtcc tgcaaactga cccaagccac gggatcggcc
    22021 tgaccgacaa ttttttgatc atctgcccag cgcgccacgc gattttgttg ataatcacgt
    22081 tttacgctcg cggcaattcc ggcagcatcc agaccttcac actttgggaa agtgaccgac
    22141 ttgctggttt cattattggc agcgaaaact gatgcgcagg cagaaaccaa caacagccct
    22201 aaaaacgccc ctcttttttt catgtttttc tccatagcac aatgattcag gagaaagcat
    22261 ggtacaaatt gtcaggagcg caagttgctt caggccgcgt gtgaggcatc ttcctcttcg
    22321 gattcagcac cgacgacctg tggggacggt aatttgccgg ttttcagaat ggtgctcagc
    22381 acatcttttt gttctgccag ccatagcgaa agggcttcac gttgctcgtc ttccatttgc
    22441 actggagaat ttgtcagcca ggtgtcaagc agatccgccg tatcaagcat tttgtcataa
    22501 gcatcggctt cctttttgct ggtaaacgac attttctctt cgccctcacg aatgactacg
    22561 tatttaactt caaccgccat ttgcagcctc tcataataac tgtgatttta tacagtatat
    22621 ttcttttcgg ttgagaaatc aacaccagca ataaagacac acgcaaacgt tttcgtttat
    22681 actgcgcgcg gaattaatca ggggatattc gttatgacgt tattaggcac tgcgctgcgt
    22741 ccagcagcaa ctcgcgtgat gttattaggc tccggtgaac tgggtaaaga agtggcaatc
    22801 gagtgtcagc gtctcggcgt agaggtgatt gccgtcgatc gctatgccga cgcaccagcc
    22861 atgcatgtcg cgcatcgctc ccatgtcatt aatatgcttg atggtgatgc attacgccgt
    22921 gtggttgaac tggaaaaacc acattatatc gtgccggaga tcgaagctat tgccaccgat
    22981 atgctgatcc aacttgaaga ggaaggactg aatgttgtcc cctgcgctcg cgcaacgaaa
    23041 ttaacgatga atcgcgaggg tatccgtcgc ctggcggcag aagagctgca gctgcccact
    23101 tccacttatc gttttgccga tagcgaaagc cttttccgag aggcggttgc cgccattggc
    23161 tatccctgca ttgtaaaacc ggtgatgagc tcttccggca aggggcagac gtttattcgc
    23221 tctgcagagc aacttgctca ggcatgggaa tacgctcagc aaggcggtcg cgccggagcg
    23281 ggccgcgtaa ttgttgaagg cgtcgttaag tttgacttcg aaattaccct gctaaccgtc
    23341 agcgcggtgg atggcgtcca tttctgtgca ccagtaggtc atcgccagga agatggcgac
    23401 taccgtgaat cctggcaacc acagcaaatg agcccgcttg cccttgaacg tgcgcaggag
    23461 attgcccgta aagtggtgct ggcactgggc ggttatggtc tgtttggtgt cgagctgttt
    23521 gtctgtggtg atgaggtgat tttcagtgag gtctcccctc gtccacatga taccgggatg
    23581 gtgacgttaa tttctcaaga tctctcagag tttgccctgc atgtacgtgc cttcctcgga
    23641 cttccggttg gcgggatccg tcagtatggt cctgcagctt ctgccgttat tctgccacaa
    23701 ttgaccagtc agaatgtcac gtttgataat gtgcagaatg ccgtaggcgc agatttgcag
    23761 attcgtttat ttggtaagcc ggaaattgat ggcagccgtc gtctgggggt ggcactggct
    23821 actgcagaga gtgttgttga cgccattgaa cgcgcgaagc acgccgccgg acaggtaaaa
    23881 gtacagggtt aaacccggca aataaacgct acaaaaatgc ccgatcctcg atcgggcatt
    23941 ttgactttta cagcttagcg ccttctacag cttcacgcgc cagcttagta atgcggtcgt
    24001 aatcgcccgc ttccagcgca tctgccggaa ccaaccagga accaccgatg cacagcacgc
    24061 ttttcagcgc caggtagtca cggtagttag ccggagaaat accacccgtc gggcagaaac
    24121 ggacctggga gaacggaccc gcgatcgcct gcagggcttt cacgccgccg ttagcttcag
    24181 ccgggaagaa tttgaactct ttcaaaccgt agtccatacc cagcatcagt tcggaaacag
    24241 tgctgatccc cggaatcagc ggaatagtcc cttcggtagc agctttcagc agcggctcgg
    24301 tcagacccgg gctaattgcg aactgtgcac ccgcttcagt gacttctgcc agctgctgtg
    24361 gattcagcac cgtaccggca cccacaatcg cttcaggcac ttctttggcg atagcacgga
    24421 tagcgtcaac tgcacactcg gtacgcagag tcacttccag aacgcgcacc ccaccagcaa
    24481 ccaacgcttt tgccatcggc accgcgtgtt ccagtttttt taccacgata accggtacaa
    24541 ccgggccggt ggtcaggatt gattctgcac ttgttttcca gtttttcatc agagttttct
    24601 ctcgcctgat tacaaatttg tcgtcttaaa aagtgataca ggttgcgccc tgttcggcac
    24661 cggacagttt ttcacgcaag gcgctgaata attcacgtcc tgttcccacg cgtgacgcgc
    24721 tcaggtcagg aatgtgtggt tcgcgagcag ccagttccgc ttcgtctacc agcagcgtca
    24781 gttcgcctgt ctgtccattc acacgaatga tgtccccgtc gcgcactttt gccagcagcc
    24841 cgccatcgta ggcttctggt gttacgtgga tagctgacgg cactttacct gaagcgccgg
    24901 agagtcgtcc atcggtaact aacgcaattt tgaaacaccg gtccaataat acaccaagtg
    24961 gcggcatgag tttatgtaat tctggcattc cgttcgcttt tggcccctga tgacggacaa
    25021 caacgacaca atcgcggtcc agcaaacccg cttcaaaggc cggcataacg tcatgctggc
    25081 tttcaaaaac aaccgctggc gcttcaatca cctggttctc aaccggcacg gcagaggttt
    25141 tcataaccgc acggcccagg ttaccgctta acacttttgt cccaccatga tgagagaaag
    25201 gttgttcgaa ggaagcgatc acattgctgt cgagtgattt ttccgcccct tcccgccagt
    25261 ccagttcacc attattcagc catggttcaa gggtataacg agacagacca aagcctgcca
    25321 ccgtattgac atcttcatgc agcaggcctg ctttgagcag ttcacgcacc agaaccggta
    25381 cgccacctgc cgcctggaag tggttaatat cggccggacc gttcgggtag agacgagcca
    25441 tcagcggtac aacatcagaa aggtcagaga agtcatccca gttaatctga ataccggccg
    25501 cgcgcgccat cgccaccagg tgcatggtgt ggttagtgga accaccggtc gccagcagtg
    25561 caacgatacc gttcaccacc actttctcat cgatcatctt accgatcggc atccattcat
    25621 taccattacc ggtcatgcgt gtaacctgac gcgcagcggc agcggtcaaa gcatcgcgca
    25681 gcggagaatc cggatgaaca aaagaagagc ctggcaactg catccccata aactccacca
    25741 ccatctggtt ggtgttggca gtaccgtaga aagtacatgt tcccggcgca tggtaagacg
    25801 cggcttctga ctccagtaag gccatgcggt ccactttacc ttcggcataa agctggcgaa
    25861 tacgcacttt ttctttattt ggcaaaccgc ttgccatcgg tccagacggc acaaacaccg
    25921 caggcaaatg accaaacgac agggctgcca tcgtcagacc cgggacaatc ttgtcgcaca
    25981 ccccgaggaa cagagcacca tcaaacatgt tatgggacag cccgaccgcc gcagacatcg
    26041 ctatcacttc gcggcttagc agcgacaatt ccattccatc ctgcccctgg gtgacaccat
    26101 cacacatcgc cggaacacca cccgcaacct gaccaaccgc attcgcttca tgcagggctt
    26161 tacgaatgat ttctggatag tgttcataag gctggtgagc ggagagcatg tcgttatagg
    26221 aggtgatgat ggcgatattg ttacgcaaca tgcttttcaa agaggctttg tcttctggct
    26281 ggcaggcagc gaaaccgtgt gccaggttac cgcatgccaa ctgcgaacga tgaacggtcg
    26341 acgttttcgc ttgttctatc cgggcgagat aagcagagcg agtctcgcgc gaacgttcaa
    26401 tgattcgatt tgttacgcgt aacaattgtg gattcataaa ggctcctgaa attgagttgt
    26461 cagagcagga tgattcacaa cgcgtttcat tcagaggatt tatgagtgaa acgcctgtaa
    26521 ccggagctca tagggcaaaa acgtttcagt cagtgtaata aaaaaagcct cgtgggtgaa
    26581 tccgcacgag gcctgaaagt gtaaaaattg ttctacaatc tgtgcaagat catgttaccg
    26641 gtaaaataac cataaaggat aagcgcagat attactcaaa ctcattccag gaacgaccat
    26701 cacgggtaat catcgccacc gaggcaacgg gtccccaggt tccggcctga tacggtttcg
    26761 gcgcatcatt gtccatcgcc cacgcctcag taatggagtc tacccatttc caggcttctt
    26821 ccacttcatc gcgacgtaca aacagtgcct gaataccacg catggtttcc agcagcaaac
    26881 gttcataggc atccgccaga tgcgtctgat taaaggtttc tgaatagctc agatccagct
    26941 tggtgatttg caggttatgt ttgtggtcaa ggccaggaac tttattcagt acctggatat
    27001 ccacgccttc atcaggttgc agacggatag tcagtttatt ctgcggcaga tcctgccacg
    27061 actctttaaa cagattcagt tcaggtgttt tgaaatagac cacgacttca gaacatttgg
    27121 tcggcagacg tttaccagta cgcaggtaga atggcacacc ggcccagcgc cagttatcaa
    27181 tgtcgacgcg gatcgccacg aaggtttctg tattgctgct cttgttcgcg ccctcttctt
    27241 ccagatatcc cggcactttt ttgccctggg cgaagcccgc agtatattgc ccgcgtacgg
    27301 ttttttcgcg tacgttggag cggtcgatgc ggcgcagaga cttcagtact ttcacttttt
    27361 catcgcgaat gctgtctgcg ctcaggtcag acggcggaga catcgcaatc atgcaaagaa
    27421 tttgcagcag gtggttctgg atcatgtcgc gcatctgacc ggctttatca aaatagcccc
    27481 agcgcccttc gatccccact tcttctgcca cggtaatctc aacatgatca atggtgcgat
    27541 tgtcccagtt attcacaaac agggagttag caaaacgcag cgccaacagg ttcagcaccg
    27601 tttctttacc aagatagtgg tcgatacggt aaacctggca ctcctcgaag tattcgccaa
    27661 cctgatcatt gatttcctgc gaggtcgcca gcgacgtccc cagcggtttc tccatgacta
    27721 cgcgtgccgg tttagcattc agttttgcct cgccaagccc tttgcaaatt gcgccaaaag
    27781 tgctgggcgg catggcaaag tagttaatgg tgatacgatt tttttgatcc agcatcgcgc
    27841 cgagacggtt gaatgcagca gtgtcattga catcgagatt acaaaaatcc agacgtgcac
    27901 tcagggtgtc ccataaacct tcatcaatgg tttctttcat gaaagtttcg agcgcctcgc
    27961 ggacaacttt ggtatatgcc gctttatccc agtcagcacg ccctacgccg ataatccggg
    28021 tgtccgggtt gagctgaccg gctttttcca gttgatacag ggaaggcagc aatttacgac
    28081 gcgcaaggtc gcctttcgcg ccgaaaatga ccaggtcaca ggcctgggct gtttgcgtta
    28141 ccgccatgtc attctcctta agttaactaa cccggtactt aagccagggt atacttgtaa
    28201 ttttcttacg gtgcactgta ctgcttttac gagcttgcga aaactgtaaa cgcttatcca
    28261 cccgtgcgat tacggtaaaa gcgcgtaaag tgcggcaaaa ctgataaaaa aatcatcgtt
    28321 ttccattggg taaaaatctg acactgatca tgttatgaaa aaaaataaca acttttttat
    28381 ctgcttttgt cattaacaca gcacacaggc gtaatatctg acaaaactgc atttcgattt
    28441 ctttcagtgc ggaaatcgtc attacccgtg agtctcttta catcatgaat atgctggaaa
    28501 aaatccagtc tcagctggaa catttgagca aatcggagcg caaagttgcc gaggtcattc
    28561 tggcttcgcc cgataacgcg atccattcga gtattgctgc tctggcgctg gaagccaatg
    28621 ttagcgaacc gacggtgaat cgtttctgtc gcagcatgga cacgcgcggt tttcctgatt
    28681 ttaaacttca tctggcacag agtctggcga atggcactcc ctatgttaat cgcaacgtca
    28741 atgaagatga cagcgttgaa tcatacacag ggaaaatatt tgagtccgca atggcaacgc
    28801 tcgatcatgt ccgtcattca ctggataaat ctgccatcaa ccgcgccgtc gacttgctca
    28861 ctcaggcaaa aaaaatcgcc tttttcggat taggctcttc agccgccgtt gcccacgatg
    28921 cgatgaataa gttctttcgt tttaatgttc cggtggtgta ctccgatgat atcgtgctgc
    28981 aacgcatgag ttgtatgaat tgtagcgacg gagacgtggt ggtgctgatt tctcacactg
    29041 gaagaacaaa aaatctggtc gagctggcgc agctggcacg cgaaaacgac gccatggtga
    29101 ttgccctcac ctctgcgggt accccgctcg cccgggaagc aacgctggca attaccctcg
    29161 acgtaccgga agatactgac atttatatgc ccatggtttc tcgacttgca cagctgaccg
    29221 tgatagatgt gctggcgaca ggatttactt tgcgacgcgg tgcaaaattc agagataact
    29281 tgaagcgggt caaggaagcg ctgaaggaat cgcgttttga taagcagtta cttaatttaa
    29341 gtgacgatcg ctaaaagcga ctgtcactgt tctaatctta tacgacatcc gaatgagatt
    29401 aatttatcgc catcgcggcg ttatttcatt cggatttcat gttcaagcaa cacctggttg
    29461 tttcagtcaa cggagtatta catgtccaga aggcttcgca gaacaaaaat cgttaccacg
    29521 ttaggcccag caacagatcg cgataataat cttgaaaaag ttatcgcggc gggtgccaac
    29581 gttgtacgta tgaacttttc tcacggctcg cctgaagatc acaaaatgcg cgcggataaa
    29641 gttcgtgaga ttgccgcaaa actggggcgt catgtggcta ttctgggtga cctccagggg
    29701 cccaaaatcc gtgtatccac ctttaaggaa ggcaaagttt tcctcaatat tggggataaa
    29761 ttcctgctcg acgccaacct gggtaaaggt gaaggcgaca aagaaaaagt cggtatcgac
    29821 tacaaaggcc tgcctgctga cgtcgtgcct ggtgacatcc tgctgctgga cgatggtcgc
    29881 gtccagttaa aagtactgga agttcagggc atgaaagtgt tcaccgaagt caccgtcggt
    29941 ggtcccctct ccaacaataa aggtatcaac aaacttggcg gcggtttgtc ggctgaagcg
    30001 ctgaccgaaa aagacaaagc agacattaag actgcggcgt tgattggcgt agattacctg
    30061 gctgtctcct tcccacgctg tggcgaagat ctgaactatg cccgtcgcct ggcacgcgat
    30121 gcaggatgtg atgcgaaaat tgttgccaag gttgaacgtg cggaagccgt ttgcagccag
    30181 gatgcaatgg atgacatcat cctcgcctct gacgtggtaa tggttgcacg tggcgacctc
    30241 ggtgtggaaa ttggcgaccc ggaactggtc ggcattcaga aagcgttgat ccgtcgtgcg
    30301 cgtcagctaa accgagcggt aatcacggcg acccagatga tggagtcaat gattactaac
    30361 ccgatgccga cgcgtgcaga agtcatggac gtagcaaacg ccgttctgga tggtactgac
    30421 gctgtgatgc tgtctgcaga aactgccgct gggcagtatc cgtcagaaac tgttgcagcc
    30481 atggcgcgcg tttgcctggg tgcggaaaaa atcccgagca tcaacgtttc taaacaccgt
    30541 ctggacgttc agttcgacaa tgtggaagaa gctattgcca tgtcagcaat gtacgcagct
    30601 aaccacctga aaggcgttac ggcgatcatt accatgaccg aatcgggtcg taccgcgctg
    30661 atgacctccc gtatcagctc tggtctacca attttcgcca tgtcgcgcca tgaacgtacg
    30721 ctgaacctga ctgctctcta tcgtggcgtt acgccggtgc actttgatag cgctaatgac
    30781 ggcgtagcag ctgccagcga agcggttaat ctgctgcgcg ataaaggtta cttgatgtct
    30841 ggtgacctgg tgattgtcac ccagggcgac gtgatgagta ccgtgggttc tactaatacc
    30901 acgcgtattt taacggtaga gtaagtacgt tgccggatgc ggcgaaaacg ccacatccgg
    30961 cctacagttc aatgatagtt caacagattt cgaatattct gaagcaaact tgaacttatc
    31021 atcaggcgaa ggcctctcct cgcgagaggc ttttttattt gatgggataa agatctttgc
    31081 gcttatacgg ctggatttcg cccggtttgc gagttttcag caattttagt atccaggtgt
    31141 attgttctgg tcgcggacca acaaaaatct cgacttcttc attcatccgc cgcgcaatcg
    31201 tatgatcatc cgcctctaac agatcatcca tcggtgggcg cacctgaatc gtcagacgat
    31261 gcgtcttgcc atcataaatc ggaaacagcg gtacaacgcg cgcacggcac actttcatca
    31321 aacgaccaat cgcgggcaac gtcgctttat aggtggcaaa gaaatccaca aattcgctgt
    31381 gctctgggcc atgatcctga tcgggtaaat aatatcccca gtacccctga cgtaccgact
    31441 ggatgaatgg tttaataccg tcatttctcg catgcagacg accgccaaag cgacgacgca
    31501 ccgtgttcca gacataatca aaaaccgggt tgccctgatt atggaacatc gctgccattt
    31561 tctgcccttg cgaggccatc agcatggcag gaatatcgac ggcccaaccg tgcggcacca
    31621 gaaagataac tttctcgtta ttacgccgca tctcttcgat gatctccagc ccttgccagt
    31681 caactcgcgg ctgaattttc tccggcccgc gtattgccaa ctcagccatc attgccatcg
    31741 cttgcggcgc ggtggcaaac atctcatcaa caatcgcttc gcgttcagct tcactacgtt
    31801 ctggaaagca gagcgacaga ttgattaacg cacgacggcg tgagcttttt cccagtcgtc
    31861 cggcaaaacg tcccagccat gccagaatgg gatcacggaa ctttggcggc gttaaagcga
    31921 tacccgccat cgctgctacg cccagccatg ctccccagta gcgcgggtgg cgaaaggatt
    31981 tatcaaactc aggaatgtat tcgctattat tttttttcgt ttccatgctt ttccagtttc
    32041 ggataaggca aaaatcaatc tggtgatagt gtagcggcgc aacttgcccc gcaccaaata
    32101 aaaaaagccg gtactgactg cgtaccggct gcgaatggat gttaattaat caaaccgtag
    32161 ctgcggcaca atctctttgg cctgcgccag gaattcgcga cgatcggagc cggtcagccc
    32221 ttcggtacgc ggcagttttg ccgtcagcgg gtttacggcc tgctggttta tccatacttc
    32281 atagtgcaga tgcggcccgg ttgaacgtcc ggtattaccg gaaagcgcga tacggtcgcc
    32341 acgtttcacc ttctgtcccg gtttcaccag aatcttgcgc aagtgcatat aacgcgtggt
    32401 gtagctgcga ccatgacgaa tagccacata ataacctgct gcgccactgc gtttggcaac
    32461 caccacttca ccgtcaccca ctgaaagcac tggcgtaccc tgcggcatgg cgaaatcaac
    32521 acctctgtgt ggtgcaacgc gaccggtcac cggattagta cgacgcgggt taaagttaga
    32581 cgagatacgg aactgtttcg ccgtcgggaa tcgcaagaat cctttcgcca gaccagtacc
    32641 gttacgatcg tagaatttgc catcttcagc gcggatagcg taataatctt taccttctga
    32701 acgcaaacgt acgcccagca gctggctttg ctcacgttta ccatcaagca tttctcgaga
    32761 cattaatacc gcaaattcat cgcctttttt cagtttgcgg aaatccattt gccactgcat
    32821 ggctttaatc actgcgctca cttcggcgct ggttaaaccg gcgtttctgg cgctggcaac
    32881 aaagctcccc ccgacggtac ctttcagcag attgttaacc cactctcctt gctgcatttc
    32941 gctggtcatt ttaaaaccgt tagcggcagt acggtcatag gttcgggttt cacgacgaga
    33001 cacttcccag gtgaggcgct gcagttctcc gtccgcggtt aatgtccagg agagttgttg
    33061 accgattttc aggttacgca attctttgtc ggcagcagcc agttgggtga tatcacccat
    33121 atcaatacca tactgattga gaatgctgct tagcgtatcg ccagtggaaa caacatattc
    33181 atgcacgccc gcttcaccgg cgattttgtc atccagttcg tcctggggaa tggcttcatc
    33241 ttcttgtgca gcttgatcaa tcggctcact ggcttcaggt aagagcgaac gaatttcgtt
    33301 ctgttccagc tcaatggttt tgacaattgg cgtggcatcg cggtgataaa catagggccg
    33361 ccagacagcg acggccagag taagaacggt gagcgacccc aacataacgc ggtgtggtcg
    33421 cggtaaatta ttaaacgcca gggcgacaga gcgggctatc tgttgcacgt aatcacttcc
    33481 tcattaatct cctttcaggc agctcgcata ctggttggct aattgactca ggaattctga
    33541 atagcttgtt ttacccagtt tgatattcgt ccccagggga tccaacgttc ccatacgaac
    33601 ggatgtccct cgtgcgacgc tctcaacgac cgctggcctg aactgtggct cagcaaaaac
    33661 gcaggttgct ttttgctcaa ccaactgtgt tcttatttca tgtaaacgct gcgcgccagg
    33721 ttgaatctca gggttaacgg taaaatgacc aagcggtgtc agtccgaact gtttttcgaa
    33781 atagccgtaa gcatcgtgaa aaacgaaata acctttcccc ttgagcggcg cgagctcgtt
    33841 accaacctgc gtttcggttg aggctaattg tgcctcaaaa tccttcaggt tggcgtcaag
    33901 tttggctcga ctttgcggca taagttccac taattttcca tggattgcaa ccgctgtagc
    33961 ccgcgctatc tctggggaaa gccaaagatg catgttgaaa tcgccgtgat ggtgatcttc
    34021 gtcacttttt tccgcgtggt cgtgatcatc atcatcgccg tgaatacttt tcatcagtag
    34081 cggtttcaca tcttctagct gcgcaatcgt tacctgcttc gcttctggta atttgcttac
    34141 cggtttttgc ataaacgctt ccatctccgg gccaacccaa acgactaagt ccgcgttctg
    34201 taagcgtttt acatccgatg gacgcagtga ataatcatgt tctgaagcac cgtcaggcag
    34261 taaaacctcc gtttctgtta ccccatcagc aatggcagaa gcgatgaacc caacgggttt
    34321 aagcgaagcg acaacggcgg catctgcggc ctgtgttgca cctccccaga gagcggcgga
    34381 taatgctgcg aaaagaagcg tttttttatg taacataatg cgaccaatca tcgtaatgaa
    34441 tatgagaagt gtgatattat aacatttcat gactactgca agactaaaat taacatgaca
    34501 agtctggttt ccctggaaaa tgtctcggtt tcttttggcc aacgccgcgt cctctctgat
    34561 gtgtcgctgg aacttaaacc tggaaaaatt ttgactttac ttgggccaaa tggcgcaggt
    34621 aagtcgacac tggtacgggt agtgctcggg ctggtaacac ccgatgaagg ggttatcaag
    34681 cgcaacggaa aactgcgcat cggctatgta ccgcagaagc tgtatctcga caccacgttg
    34741 ccactgaccg taaaccgttt tttacgctta cgccctggta cacataaaga agatattttg
    34801 cctgcactga aacgtgtcca ggccgggcac ctgattaacg caccgatgca aaagctctcg
    34861 ggtggcgaaa cgcagcgtgt actgttagcg cgagcattgt taaatcgccc gcaattatta
    34921 gtgctggatg aacccactca aggcgtggat gtaaatggcc aggtggcgtt atatgacctt
    34981 attgaccaac tgcgtcgtga actggattgt ggcgttttaa tggtctctca cgatctgcat
    35041 ctggtaatgg caaaaaccga tgaagtgctg tgcctgaatc accacatttg ttgttccggc
    35101 acaccggaag ttgtttccct gcatccggag tttatttcaa tgtttggtcc tcgtggtgct
    35161 gaacaactgg gtatctatcg ccatcatcat aatcatcgtc acgatttaca gggacgaatt
    35221 gttttgcgtc ggggaaatga tcgctcatga ttgaattatt atttcccggt tggttagccg
    35281 gaatcatgct cgcctgtgcc gcgggtccgc tgggttcgtt tgtggtctgg cgtcgtatgt
    35341 cttatttcgg tgatacgctg gctcatgcct cattacttgg tgtcgcgttt ggtttgttgc
    35401 tggacgtgaa tccattctat gcggtgattg ccgttacgct gctgctggcg ggcggtctgg
    35461 tatggctgga gaagcgtcca cagctggcga tcgacacgtt attagggatt atggcgcaca
    35521 gtgccctgtc gctgggcctg gtggtcgtta gtctgatgtc taacattcgt gttgatttga
    35581 tggcttacct gttcggtgat ttactggcag tgacgccaga agatctcatc tctattgcaa
    35641 ttggcgtggt catcgtggtg gctattttgt tctggcaatg gcgcaatttg ctgtcgatga
    35701 cgattagccc ggatctggcg tttgttgatg gtgtgaaatt acagcgagtg aaattgttgt
    35761 tgatgctggt gacggcattg acgattggtg tagcgatgaa atttgtcggt gcgttgatta
    35821 ttacttcgtt gctgattatt cctgcagcta ctgcgcgtcg ctttgcccgt acgccggaac
    35881 agatggctgg tgtcgctgtt ttggtgggga tggtggcagt aactggcggt ttaacctttt
    35941 ccgcgtttta cgatacgccg gcgggtccgt cggtggtgct atgtgcggca ctgttattta
    36001 ttatcagtat gatgaaaaag caggccagct aatctatcgc tgaacacatt tgtcggatgc
    36061 ggcgcgagcg ccttatccga cctacggttc gctatctctg gtaggcctgg taagacgcga
    36121 acagcggcgc gagcgccttt tccgccctac ggttcgctat ctctggtata tgcctgataa
    36181 gacgcgaata gcgtcgcatc aggcacactg ccaatgtcgg atgcggtgcg agcgaccaat
    36241 ccgatttacg gcatttctgg cggcgttatg ccaaagtgat tccacgcccg cgtcgtcgcc
    36301 atacgcccgc gcggtgtacg ctgcaaaaag ccttgctgaa tcaaataagg ttccagcaca
    36361 tcctcaatgg tttcacgttc ttcgccaatg gctgccgcca ggttatccag acctaccggc
    36421 ccaccaaaga acttatcgat taccgccagc aacaatttgc ggtccatata atcgaaacct
    36481 tcagcatcga cattcaacat atccagcgcc tgagcagcga tatctgccga aatagtgcca
    36541 tcgtgcttca cttcggcgaa atcacgcact cgacgcagca gacggttggc aatgcgcggc
    36601 gtaccgcgag cgcgacgagc aacttccagc gcgccgtcat cactcatctc aagccccata
    36661 aagcgtgcgc tgcgactgac gatatattgc agatccggca cctgataaaa ctccagacgt
    36721 tgcacaatac caaaacggtc gcgcaacggt gatgtcagcg aacctgcgcg cgtggttgca
    36781 ccaatcaggg taaacggcgg caaatcaatt ttaatggagc gtgccgccgg accttcacca
    36841 atcatgatat ccagttggta gtcttccatt gccgggtaca gcacttcttc aacaactggc
    36901 gataaacggt ggatctcatc aataaacagc acgtcatgcg gttcaaggtt agtgagcatc
    36961 gcagccaaat cgcccgcctt ttccagcacc ggaccagaag tcgtgcgtaa attaacgccc
    37021 atttcattgg cgacaatgtt ggcaagcgta gttttaccca accccggagg accaaaaatc
    37081 aacaaatgat cgagggcatc gccgcgcagt ttcgctgctt tgatgaaaat ctccatctgt
    37141 gaacgaacct gcggctgacc aacatactct tccagtaatt tagggcgaat ggcgcgatct
    37201 gctacatctt ccggcaaagt ggtaccggca gaaatcagac ggtctgcttc aatcatcctt
    37261 tacctcataa tgcggcgcgt agggcttcgc gaattaatgt ttcactgctg gcgtcagggc
    37321 gagcgatttt gctcaccatg cggcttgctt cttgtggttt atagcccagc gccactagcg
    37381 cagcaaccgc ttcttgttca gcatcgtcgg tcgccgggct agcaggagac gtgagtacca
    37441 ggtcggcggc tggcgtaaag agatcgccat gcaaaccttt aaatcggtct ttcatttcca
    37501 caatcaagcg ttcggcggtt tttttgccaa tacccggcag tttcaccagt gcccccactt
    37561 cttcacgctc aacggcatta acgaactgct gcgctgacat tccggagagg atcgccagcg
    37621 ccaacttcgg gccgacgccg ttggttttga tcaactcttt gaacaatgtg cgctcttgtt
    37681 tattgttaaa accgtacagc agttgcgcgt cttcacgcac cacaaagtgg gtgaaaacga
    37741 tcgcttcctg acccgcttca gggagttcat aaaaacaggt catcggcata tgcacttcat
    37801 agcctacgcc gcccacttca attaacacca gcgggggttg tttttcaatg atgatgcctc
    37861 tgagtctgcc tatcacatga cgctcctgcg taatgaatca aagataatgc tgtatgataa
    37921 aaaaatgctg gatagatatc cagcgaagga tgaagaaaac ttgcgaggtg tctcgatgaa
    37981 ctgaaaaatg
//
LOCUS       DAZFFG010000032        37929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFFG010000032 DAZFFG010000000
VERSION     DAZFFG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.032"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            132..2375
                     /gene="pdeF"
                     /locus_tag="ACHJ7J_002012"
     CDS             132..2375
                     /gene="pdeF"
                     /locus_tag="ACHJ7J_002012"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO5000249.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2414..3955)
                     /gene="ppx"
                     /locus_tag="ACHJ7J_002013"
     CDS             complement(2414..3955)
                     /gene="ppx"
                     /locus_tag="ACHJ7J_002013"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO5000250.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3960..6026)
                     /gene="ppk1"
                     /locus_tag="ACHJ7J_002014"
     CDS             complement(3960..6026)
                     /gene="ppk1"
                     /locus_tag="ACHJ7J_002014"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO5000251.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6197..6835)
                     /gene="purN"
                     /locus_tag="ACHJ7J_002015"
     CDS             complement(6197..6835)
                     /gene="purN"
                     /locus_tag="ACHJ7J_002015"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO5000252.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6835..7872)
                     /gene="purM"
                     /locus_tag="ACHJ7J_002016"
     CDS             complement(6835..7872)
                     /gene="purM"
                     /locus_tag="ACHJ7J_002016"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO5000253.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8197..8823
                     /gene="upp"
                     /locus_tag="ACHJ7J_002017"
     CDS             8197..8823
                     /gene="upp"
                     /locus_tag="ACHJ7J_002017"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO5000254.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8909..10198
                     /gene="uraA"
                     /locus_tag="ACHJ7J_002018"
     CDS             8909..10198
                     /gene="uraA"
                     /locus_tag="ACHJ7J_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO5000255.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10293..10994
                     /gene="hda"
                     /locus_tag="ACHJ7J_002019"
     CDS             10293..10994
                     /gene="hda"
                     /locus_tag="ACHJ7J_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO5000256.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11132..11491)
                     /gene="arsC"
                     /locus_tag="ACHJ7J_002020"
     CDS             complement(11132..11491)
                     /gene="arsC"
                     /locus_tag="ACHJ7J_002020"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO5000257.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11512..12975)
                     /gene="bepA"
                     /locus_tag="ACHJ7J_002021"
     CDS             complement(11512..12975)
                     /gene="bepA"
                     /locus_tag="ACHJ7J_002021"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO5000258.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13188..14249
                     /gene="yfgO"
                     /locus_tag="ACHJ7J_002022"
     CDS             13188..14249
                     /gene="yfgO"
                     /locus_tag="ACHJ7J_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5000259.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14287..15135)
                     /gene="focB"
                     /locus_tag="ACHJ7J_002023"
     CDS             complement(14287..15135)
                     /gene="focB"
                     /locus_tag="ACHJ7J_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO5000260.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15157..17169)
                     /gene="hyfR"
                     /locus_tag="ACHJ7J_002024"
     CDS             complement(15157..17169)
                     /gene="hyfR"
                     /locus_tag="ACHJ7J_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO5000261.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAHEIHHFFGLASVCMVLGDHRKNEKFSLWCSVFSASDCACL
                     PRNMPGESVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17199..17612)
                     /gene="hyfJ"
                     /locus_tag="ACHJ7J_002025"
     CDS             complement(17199..17612)
                     /gene="hyfJ"
                     /locus_tag="ACHJ7J_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO5000262.1"
                     /translation="MTEECGEIVFWTLRKKFIASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPQTEYEDWLAQVEEKQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSAWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17605..18363)
                     /gene="hyfI"
                     /locus_tag="ACHJ7J_002026"
     CDS             complement(17605..18363)
                     /gene="hyfI"
                     /locus_tag="ACHJ7J_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO5000263.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18360..18905)
                     /gene="hyfH"
                     /locus_tag="ACHJ7J_002027"
     CDS             complement(18360..18905)
                     /gene="hyfH"
                     /locus_tag="ACHJ7J_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO5000264.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18915..20630)
                     /gene="hyfG"
                     /locus_tag="ACHJ7J_002028"
     CDS             complement(18915..20630)
                     /gene="hyfG"
                     /locus_tag="ACHJ7J_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO5000265.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEQTTDTETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALRIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20620..22200)
                     /gene="hyfF"
                     /locus_tag="ACHJ7J_002029"
     CDS             complement(20620..22200)
                     /gene="hyfF"
                     /locus_tag="ACHJ7J_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO5000266.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHCLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKYSER"
     gene            complement(22205..22855)
                     /gene="hyfE"
                     /locus_tag="ACHJ7J_002030"
     CDS             complement(22205..22855)
                     /gene="hyfE"
                     /locus_tag="ACHJ7J_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO5000267.1"
                     /translation="MTGSTIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22867..24306)
                     /gene="hyfD"
                     /locus_tag="ACHJ7J_002031"
     CDS             complement(22867..24306)
                     /gene="hyfD"
                     /locus_tag="ACHJ7J_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO5000268.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPALILLMIESVACFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24323..25270)
                     /gene="hyfC"
                     /locus_tag="ACHJ7J_002032"
     CDS             complement(24323..25270)
                     /gene="hyfC"
                     /locus_tag="ACHJ7J_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO5000269.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25281..27299)
                     /gene="hyfB"
                     /locus_tag="ACHJ7J_002033"
     CDS             complement(25281..27299)
                     /gene="hyfB"
                     /locus_tag="ACHJ7J_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO5000270.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTD
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27299..27916)
                     /gene="hyfA"
                     /locus_tag="ACHJ7J_002034"
     CDS             complement(27299..27916)
                     /gene="hyfA"
                     /locus_tag="ACHJ7J_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO5000271.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITGDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28169..28639)
                     /gene="bcp"
                     /locus_tag="ACHJ7J_002035"
     CDS             complement(28169..28639)
                     /gene="bcp"
                     /locus_tag="ACHJ7J_002035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO5000272.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28639..29211)
                     /gene="gcvR"
                     /locus_tag="ACHJ7J_002036"
     CDS             complement(28639..29211)
                     /gene="gcvR"
                     /locus_tag="ACHJ7J_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO5000273.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29357..30235
                     /gene="dapA"
                     /locus_tag="ACHJ7J_002037"
     CDS             29357..30235
                     /gene="dapA"
                     /locus_tag="ACHJ7J_002037"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO5000274.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30252..31286
                     /gene="bamC"
                     /locus_tag="ACHJ7J_002038"
     CDS             30252..31286
                     /gene="bamC"
                     /locus_tag="ACHJ7J_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO5000275.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31499..32212
                     /gene="purC"
                     /locus_tag="ACHJ7J_002039"
     CDS             31499..32212
                     /gene="purC"
                     /locus_tag="ACHJ7J_002039"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO5000276.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFE
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32380..33243
                     /gene="ypfJ"
                     /locus_tag="ACHJ7J_002040"
     CDS             32380..33243
                     /gene="ypfJ"
                     /locus_tag="ACHJ7J_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO5000277.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33258..35273
                     /gene="tmcA"
                     /locus_tag="ACHJ7J_002041"
     CDS             33258..35273
                     /gene="tmcA"
                     /locus_tag="ACHJ7J_002041"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO5000278.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFGDENFTHTPQGNIVISAFEQTLWRIEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQQNASDAQ
                     LCTSLKLSGRKLLLVRQREEAAQALFALNDVRTERLSDRITQWQFFH"
     gene            35347..36045
                     /gene="ypfH"
                     /locus_tag="ACHJ7J_002042"
     CDS             35347..36045
                     /gene="ypfH"
                     /locus_tag="ACHJ7J_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5000279.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPDLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36126..36326)
                     /gene="ypfN"
                     /locus_tag="ACHJ7J_002043"
     CDS             complement(36126..36326)
                     /gene="ypfN"
                     /locus_tag="ACHJ7J_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO5000280.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36354..37481)
                     /gene="dapE"
                     /locus_tag="ACHJ7J_002044"
     CDS             complement(36354..37481)
                     /gene="dapE"
                     /locus_tag="ACHJ7J_002044"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO5000281.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37485..37841)
                     /gene="yffB"
                     /locus_tag="ACHJ7J_002045"
     CDS             complement(37485..37841)
                     /gene="yffB"
                     /locus_tag="ACHJ7J_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO5000282.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9211 a         9820 c         9994 g         8904 t
ORIGIN      
        1 tcaattggaa ctttcatatg acacttagaa tcaatgctct ctatagtgat gaataatcat
       61 cattcgaagt caggtgggat gcctgtctga atacacctcc ttcaggatgt gggggattct
      121 gctgagcatc tatgaaactg aatgcaactt atataaaaat acgtgataaa tggtgggggc
      181 ttccgctgtt cctgccttct ttaatcttgc ccattttcgc ccacattaat actttcgcgc
      241 atatttcttc cggtgaggtt tttctctttt atctgccttt ggcactgatg atcagcatga
      301 tgatgttttt cagctgggcg gcattgccag ggatcgcctt agggattttt gtccgcaaat
      361 atgcagagct tggtttttac gaaacgctct cattaacggc taattttatt atcattatca
      421 ttctctgttg gggcggttac agggtcttta ctccccgccg taacaacgtt tcacatggtg
      481 atacccgttt aatttcccag cgtatattct ggcagattgt gtttcctgca acgctgtttc
      541 tgatactttt ccagtttgct gcgtttgtag gattactggc gagcagagaa aatctggtcg
      601 gcgtcatgcc ttttaacctc gggaccttaa tcaattatca ggccttactg gtgggtaatc
      661 tgatcggtgt cccgctgtgc tacttcatca ttcgggtagt gcgaaatcca ttttatttac
      721 gtagctatta ttcgcaatta aaacagcagg ttgatgtcaa agtcaccaaa aaagagttcg
      781 cactctggct actggcatta ggtgctttac tgttgctgtt atgcatgccg ttaaatgaaa
      841 aaagcacaat ttttagcacc aattatacct tgtcattatt gctgcccctg atgatgtggg
      901 gagcgatgcg ctatggttat aagctgattt cgctgctctg ggcggtcgtg ttgatgatca
      961 gcatccacag ctatcaaaat tacattccca tttatcctgg ctataccacg cagctgacca
     1021 taacctcctc cagttatctg gtattctctt ttattgtcaa ttatatggct gtactggcaa
     1081 cccgtcagag aacggtagtc agacgcattc agcggcttgc gtatgtggac ccggtggttc
     1141 atctgccaaa tgttcgcgcc ctgaatcgcg cgttgcgtga tgccccctgg tctgcgcttt
     1201 gttatttacg catccctggc atggaaatgc tggttaagaa ctatggcatc atgctgcgga
     1261 ttcaatacaa gcaaaaactt tctcactggc tgtcaccctt gctggaaccg ggtgaagatg
     1321 tttatcagct ttcgggtaac gatctcgcgc tgcgactgaa tacagaatcg caccaggagc
     1381 gcattaccgc actggatagc catctcaagc aatttcgttt cttttgggat ggaatgccga
     1441 tgcaaccgca gattggcgtc agttactgct atgtgcgctc gccagtgaat catatctacc
     1501 tgctgctggg agagctaaat acggtcgccg aactttccat cgtgaccaac gccccggaaa
     1561 atatgcagcg tcgcggggca atgtatttgc aacgcgaatt gaaagataaa gtcgcgatga
     1621 tgaatcgact acagcaggcg ctggaacaca accatttttt cctgatggcc cagccgatta
     1681 ccggtatgcg tggtgatgtt taccatgaaa ttcttctgcg catgaaaggt gagaatgatg
     1741 aactgatcag ccccgatagc ttcttgccgg tcgcgcacga atttggttta tcgtcgagta
     1801 tcgacatgtg ggtcattgag catacgctgc aatttatggc tgaaaacaga gcgaagatgc
     1861 ccgctcaccg ttttgctatt aatctgtctc caacctcggt atgtcaggct cgttttcctg
     1921 ttgaagtcag tcagttgctg gctaaatatc agattgaagc gtggcaactt atttttgaag
     1981 tcaccgaaag taatgctctg accaatgtta agcaggcgca aatcaccttg cagcatcttc
     2041 aggaattagg ctgccagatt gcgattgatg atttcggcac cggctacgcc agctatgcgc
     2101 ggcttaaaaa tgtgaatgcc gatctgctta aaattgacgg cagttttatc cgcaatattg
     2161 tgtcaaatag tctggattat cagatagtgg catcgatttg ccacctggcg cgaatgaaga
     2221 aaatgcgggt agtggcagag tacgttgaaa acgaagagat ccgcgaggcg gtgctctctt
     2281 tggggatcga ttatatgcag ggttatctta ttggtaagcc gcagccgtta attgatacgt
     2341 tgaatgaaat cgaacccatt cgcgaaagtg cctgaataat gcgggccgac atttctcgtc
     2401 ggcccgcaaa gcattaagcg gcgatttcag gtgtactttc ttcttcaatt ttcaaccgcc
     2461 agccagccac gccttcccag tattcttgct ccttttccag atcaagcagt accaacgcat
     2521 tctgactaaa ccagtcatgc gggaaacgca gtgtccagtg actgtcatca gtaatcagtg
     2581 tcaatgttgg cggtgtggtg gttgcctgac gttgattgtt gagtaatacg ccaaggcgca
     2641 atagctgaat cagtggcagg aactgtttct tcttaaacaa ggtaaagcgc ggcagatcgt
     2701 cgagtttaat cgctttacgg tgatagcgca ccagtgtcgc catcatcagc tgctgttcct
     2761 gattaaaacc cggcaagtca ctgttttgca gaatataagc ggagtggcga tgcaaaccgc
     2821 tgtggttgat attcaacccg acctcatgca gcatggcggc ccatcgcagt agcgcctcca
     2881 gttgcggatg cgccagcttc ggttgctgtt cccgccactg ttcgtacatt tgcatagtgg
     2941 tatccagcac ccgtcgggcc tgttcgctgt cgatgtgata ctggttggcg aggctgctgg
     3001 cggtgcgact acgcacatcc tgatgacgga aacgtccttc catttcatac agtacgcctt
     3061 cgcgtaacgc cccgtcagaa aggcgcagtt cacggatggc taaagcatca aacacaccgc
     3121 ataaaatcgc cagtcccgga acgaagactg ttttccgctc ttcggaaaga cctggtaaac
     3181 tcagcgatgc gaaattacgg tgacgtaaaa cttcttttac cagtttttcc agacgttccg
     3241 gggtaattat cccgtctttc tcgcccattt ccattaatac ttcatgggcg gcttttatgg
     3301 taccggaagc gcccattgcc acgttccagc cctgaatacg gaattgccag gttaaagttt
     3361 ccagtttttg tgctgccgcc atgcgagcac gctgaaaatt ctctttattg atgactccgc
     3421 cagggaaata aagctgggca aagctgacac accccatccg gcggctttca acgagaatcg
     3481 gttcgaaatt ttcaccaatc accagttctg tagaaccgcc gccaatatca ataaccagtt
     3541 tgcgaccttt ttccggttgg gtatgttcca cgcccataaa aattagacgg gcttcttcat
     3601 taccggaaat aatttcaatc gggtagggaa tgaccttttc cgcgcgtttc agaaagtcag
     3661 tggcgttcag cgcctgacgc agcgtatggg taccaactat acagacgctg gcaggagaaa
     3721 acccttgtag ccgttcggca aacagcgaca gacagttcaa accgcgcgtc attgcctctt
     3781 cactcaacat attatctggc cccaggccgt ccgccagatg cacccgctgt ttcaggcggc
     3841 caataatctg catggcacca tctaccacac gggctatgac catgtgaaaa ctgtttgaac
     3901 caagatcgac cgcagcaaac tcctgcggac gaggggattt atcgtgtatt ggcatagggt
     3961 tattcaggtt gttcgagtga tttgatgtag tcataaatcg ccaactgcgc ccgtactttg
     4021 cggcgattgc cgcggggaac gtagcgatta ctgagttctt tatcgatata acgtgctttg
     4081 accgtatcgc tgaacaatat gtcgatgatg tccagcaccc gctgcttcag gcgcggatcg
     4141 agcagcggcg tcgccacttc aatacgataa tcaatattgc gcgtcatcca gtcggcagaa
     4201 gaaaggtaga cctttttatc gccgccattt tcaaaaatat aaacccggtc atgttcaagg
     4261 taacggtcaa caatactgat ggcacgaatg ttgtcgctaa tgccttccag attggggatc
     4321 agcgaacaca ttccgcgaac cagcagatta accggtacgc cggagctgga ggccgcatac
     4381 agacgatcaa ccaggccttt atcgacaagg ttatttagct tcagggtgat accactgggc
     4441 agcccttgct gcgcgttggc gatctcgcgg tccaccattt catacaacag gcggcgggag
     4501 ttttgcggcg acaccattaa ataatcaaat gtcaccggac ggtatgggtt ttcaataaag
     4561 ttaaataccc gccgtacttc gttggtgatg cgcgcatcgg cggtcagcaa cgaatagtca
     4621 gtataaagac gcgcggtttt ttcgttaaag ttcccggtcc cgatgtgtgc ataacgcacc
     4681 acttcaccgt tttctttacg tgaaatcagg aacagtttgg catgaatttt cagccccggc
     4741 gcagagaaga taacgtgcac gcctgcttcg gtcaggcgct tcgcccagtg aatgttggct
     4801 tcttcgtcaa aacgtgcctg tagctcaacc accacggtca ctttcttgcc gttgtgcgca
     4861 gcgtggatca tcgagtcgat gatgcgtgaa tcttttgcca cacggtaaat gttgatttta
     4921 atcgccagta cgctcgggtc gaacgaagcc tgacgcagca gttccagcac atgctcaaag
     4981 gtgtgataag gataatagag caacacatcg cgttcgcgaa tggcatcaaa accattgcgg
     5041 aactgggctt tatcaaacca aatatggcgt aaacgcggta gtggtttgtt caccagattg
     5101 gctttgccga cattggggaa attaataaag tctttaaaat tatgataacg accaccgggg
     5161 acgatggagt cgtagcggga aatagtcagt ttttcgcgta acacttcaac cagcgcattg
     5221 ggcatatcgc gctgataaac aaaacgcacc ggctcagcag ttaaacgctg cttgagactg
     5281 gaagacatca actccatcag gctggcttcc atctcatgca ctaaatcgta ttctgcatcg
     5341 cgggtcatct tcattgaata ggcattcagc gcgtcataat caaagaagcc tttgaaaata
     5401 tcatcaaggc agtaacgcag aatgttatcc agaagaatca tcggcttgcg tcgacgcggt
     5461 gcttctggcg gtaaattcac aaagcgcggc actttatctg atgggatctc cagcagtgcg
     5521 taacggatgg tatcgccacg gataatttcc accgccagat aggtgtaatc atctttcagg
     5581 aactgcacta agtcagtgtc aggattgatt aaaatcggcg taatgtgctg acgcagatac
     5641 tgcttaaaat aatgacgcag ccagttttgt tgattgacgg agagctggcg ttcattaatc
     5701 aggaagatct ggttgcgcgc catctccagc aatagctcgt tgtagaggcc gtcgaattcc
     5761 tgatcggctt tcagcacccg ggactgaatt ttgcccagta aatggcggga atgagagttg
     5821 gagccttgtt cttcgctaat aatgatgcgt cgcttcagtt cagcgaagcg gactttatag
     5881 aactcatcaa ggttattgga atagatcccc aggaaacgca tcctttcaat cagcgggtta
     5941 gatttgtccg ccgcttcctg aagcacgcgt tcattgaacg ataaccaact gagctctttt
     6001 tcgatgtata gcttttcctg acccattacc acttttactc cgttttattc acgggacact
     6061 gcctggatat tatggcgagc aatattggca gatgtccaac tatgccagaa aagggggaaa
     6121 aaaactgctg gagcttgcga tttacaggtg aattgaaaaa cgccagcggc agagctggcg
     6181 ctttaactac ggggcattac tcgtcggcag cgtagccctg cggcggcaga cgttgaccat
     6241 ccagccacgc ggcgttttcg tgcattttca gacgaccatc ggcaaaccag ctaatcacca
     6301 gtggataaat ggcgtgttcc tgggtttgca cgcgggcggt gacgtcatct tccgtatcac
     6361 cagcaaatac cgggactttc gcctgtaaaa taaccgggcc accgtccagt tcatcggtga
     6421 caaaatgcac cgatgtaccg tgctcttcat cgccattttc cagcgcctga cgatgggtgt
     6481 gtaatccggg atatttcggc agcagagaag ggtgaatgtt cagcaaacgc ccggcatagt
     6541 gggagacaaa cgccgggctg agaatgcgca taaaaccagc cagcacgacc acatcgggtg
     6601 cgtacatgtc gatttcatga atcaactccc ggtcataggc ttcacgactg tcaaacgcgc
     6661 tggcgatgag cgtatgcgtt gcaatacccg cctggcgggc gcgttcaagg ccgaacgcgt
     6721 cggccttatt gctgaaaact gcccgtacgg tgcctttaat tttgttggtt ttacaggcgt
     6781 caataattgc ctgtaaatta cttccgttgc cggaaataag caccacaata ttcattattc
     6841 gataaccacg cgttgttcgg aatcagaggc tttgatgata ccgattttcc acgcgttttc
     6901 accgttggca tttagcaggg cgagggcttt gtccacttcc ggagcaggca gggcgataat
     6961 catcccgacg ccgcagttga aggtgcgata catttcatgg cgctcaacgt tacctgccgt
     7021 ttgcagccag ttgaacactt ccggccactg ccaggatgat tcatcaatca ctgcctgggt
     7081 attatctggc aatacgcgcg gaatgttttc ccagaagccg ccgccggtca ggtgcgcaat
     7141 ggcatgcaca tcgacctttt caatcaactc cagcactgac ttcacgtaaa tgcgggtcgg
     7201 tgccagcaga tgatcggcta atggcttacc atcaagttcg gtggtttgcg gatcacaacc
     7261 gctgacttca agaattttgc gcaccagcga ataaccgttc gagtgcggac cgctggaacc
     7321 gagtgcaatc agcacatcgc cgtcgctgac tttagagccg tcgatgattt ctgatttttc
     7381 taccacgccc acgcagaaac ccgcgacatc gtaatcctca ccgtgataca tccccggcat
     7441 ttctgccgtt tcgccaccca ccagtgaaca gcccgattgc agacaacctt ccgcaatgcc
     7501 gctgatcacc gctgaagcgg tatcaacatc cagttttccg gttgcgtaat agtcgaggaa
     7561 aaacagcggt tctgcacctt gcaccaccag gtcattaacg cacatggcga ccagatcaat
     7621 accaatggtg tcgtgacgtt ttaagtccat tgccagacgc agcttggtac ctacgccgtc
     7681 agtgccggaa accagcacgg gttcacgata tttttgcggc aatgcacaca gcgcaccgaa
     7741 gccgcccaga ccgcccatca cttccggacg acgcgttttc ttcactacgc ctttgattct
     7801 tccaaccaga gcattacccg cgtcaatatc aacaccggca tctttgtagc taagagaggt
     7861 tttatcggtc actgcttggg tccccacgcg ttacttgcgg tagaaaaata aaattcggcg
     7921 caattctaac agggaaagca aacgtttgcg agactgcttt acacaacctt tttgcacgtc
     7981 ttttccccag gcgcgcggcg aaagaagact tgtgccaggg caaaggttag ttttcggatg
     8041 gaataatctt ctttcataac catttgaata taaaataact ttatctcaaa ccgttatcat
     8101 tttgactaaa gtcaacgaaa ataatattgc cgccttgaag aaaggaggta taatccgtcg
     8161 attttttttg tggctgcccc tcaaaggaga aagagtatga agatcgtgga agtcaaacac
     8221 ccactcgtca aacacaagct gggactgatg cgtgagcaag atatcagcac caagcgcttt
     8281 cgcgaactcg cttccgaagt gggtagcctg ctgacttacg aagcgaccgc cgacctcgaa
     8341 acggaaaaag taactatcga aggctggaac ggcccggtag aaatcgacca gatcaaaggt
     8401 aagaaaatta ccgttgtgcc aattctgcgt gcgggtctgg ggatgatgga cggtgtgctg
     8461 gaaaacgttc cgagcgcgcg catcagcgtt gtcggtatgt accgtaatga agaaacgctg
     8521 gagccggtac cgtacttcca gaaactggtt tctaacatcg atgagcgtat ggcgctgatc
     8581 gttgacccaa tgctggcaac tggtggttcc gttattgcga ccatcgacct gctgaaaaaa
     8641 gcgggctgca gcagcatcaa agttctggtg ctggtggctg caccagaagg tatcgctgcg
     8701 ctggaaaaag cgcatccgga cgtcgaactg tataccgcat cgattgatca gggactgaac
     8761 gagcacggat acattattcc gggcctcggc gatgccggtg acaaaatctt tggtacgaaa
     8821 taaagaataa aaataattaa agccgacttt atgagtcggc ttttttttga gtaaagcgcc
     8881 tataacacat aatacagagg ataatactat gacgcgccgt gctatcgggg tgagtgaaag
     8941 accgccactt ttacagacaa tcccgcttag tttgcaacat ttgttcgcca tgtttggtgc
     9001 aaccgtcctg gtgcccgtct tatttcatat taacccggcg actgtactgt tatttaacgg
     9061 tattggaacg ctgctgtatc tcttcatctg taaagggaaa attccggctt atcttggttc
     9121 cagctttgcc tttatttcac cggtattgtt actgttgccg ttagggtatg aagtcgcgct
     9181 gggcggcttt attatgtgcg gcgtgctgtt ctgccttgtt tcttttatcg tgaagaaagc
     9241 ggggaccggc tggctggacg tgctgtttcc acctgcggca atgggcgcaa tcgttgccgt
     9301 catcggtctg gagctggcgg gcgtagctgc cggtatggcg ggtttactcc cggctgaagg
     9361 gcaaacgcca gactccaaaa ccatcatcat ctctattacc accctggcgg tcacggtttt
     9421 aggttccgtg ctgtttcgtg gtttcctggc aattatcccg attttaattg gcgtgctggt
     9481 ggggtacgcg ctctctttcg caatgggaat tgtcgatacc acgccgatta ttaatgctca
     9541 ctggtttgcg ctgccaaccc tctatacgcc gcgcttcgag tggtttgcca ttctgactat
     9601 tctgccagcg gcgttagtgg ttattgccga acacgtaggg cacctggtag taacggctaa
     9661 tatcgtcaaa aaagatctgc tgcgcgatcc aggtctgcac cgttcgatgt ttgctaacgg
     9721 cctgtcgacc gtgatctccg gcttctttgg ctctacgcca aataccactt acggagaaaa
     9781 cattggcgtg atggcgatca cccgtgttta cagtacctgg gttatcggcg gggcggcgat
     9841 tttcgctatc ctgctttcct gcgtcggtaa actggctgcc gctatccaga tgatcccatt
     9901 gccggtgatg ggcggcgttt cgctgctgct ttatggtgtc atcggtgctt ccggtattcg
     9961 tgttttgatc gaatcgaaag tggattacaa caaagcacag aacctgatcc tgacttccgt
    10021 gattttgatc atcggcgtca gtggcgcgaa ggtaaacatc ggcgcggcgg agttgaaagg
    10081 tatggcgctg gcgaccatcg tcggtatcgg cttaagtttg attttcaaac tgatcagtgt
    10141 gctgcgccct gaagaagtgg tgctggacgc agaagacgcg gatataacag acaaataatg
    10201 taccgaccgg gcagtgttgc tgcccggttc aaacatcatg gcattctgtg gtaaacttct
    10261 cgcgattttg tgaaatcctg gttgaggtat ctctgaacac accggcacag ctctctttgc
    10321 cactttatct tcctgacgac gaaacctttg caagtttctg gccgggggat aactcctctt
    10381 tactggccgc gctgcaaaac gtgctgcgtc aggaacatag cggttacatc tatctctggg
    10441 cacgcgaagg cgcggggcgc agccatctgc tgcacgcggc ttgcgcggaa ttgtcgcagc
    10501 gtggcgatgc ggtgggctat gtcccgctgg ataaacgcac ctggtttgtt ccggaagtgc
    10561 tcgacggtat ggagcatttg tcgctggtct gtatcgacaa cattgagtgt attgcaggcg
    10621 atgagttgtg ggagatggcg attttcgatc tctacaatcg aattctggaa tcgggcaaaa
    10681 cacggttgtt gatcaccggc gatcgtccac cgcggcagtt gaatctggga ttaccggatc
    10741 tcgcgtcgcg actcgactgg gggcagatct acaaattgca gccactttct gatgaagata
    10801 agttgcaggc gctacagtta cgcgcgcgtt tgcgtggttt tgaactgccg gaagatgtgg
    10861 ggcgtttctt gctgaagcgg ctcgacagag aaatgcgcac gctatttatg acgttggatc
    10921 agttggatcg tgcgtcgatt accgcgcaac gtaaattgac cattccgttt gtgaaagaaa
    10981 ttctgaagtt gtagataagg tgtttattgt cggatgcggc gcgaacgcct tatccgaact
    11041 acgcgaagtt aatccatagt attcgtaggc cggataagac gtggcaagcg tcgcatccgg
    11101 caatgtgctt caactagcga tggagcacat cttaacccac gatttccagt acctgctccg
    11161 gtggacgacc aatccgcgct ttgccattcg cgaccacaat cgggcgctcc atcagcttcg
    11221 gattatccac cattgcctga attagcgcct cttcgctaag cgaactgtca gccaggttca
    11281 gttctttata aagatcctct ttctggcgca tcaattcacg ggcgctattc atccccagca
    11341 ttttcagcaa atcgcgcagc gttgccgcat cggcgggtgt ctcaaggtag agcaccactt
    11401 ctggctccac gccgttttct ttcagcaggt tcagcgtttc ccggctcttt gaacagcgtg
    11461 ggttatggta gatttttacc tgtttggtca ttgttcttcc tttaatgcga attacatctt
    11521 ggtataaggc ttaaagcgtt cctgcagctg gcgcaactgg tcgatgcgcg catcgtaacg
    11581 cgcttgttgc aggctgccta atttcacctg cgaactggcg ctactcaaca gcgaaatagc
    11641 ctgatcgagt cgcccggcga gcgcataacc ttctgctcgc gcagccagtt cctgatcgcg
    11701 gttatttagc gcggcttccg cctgtgccag taaatcccag ccgttactgt catctttatt
    11761 attaaaggtg tagcgattaa gaatattggc cgcttcttgt ggttgaccgc cttgtagata
    11821 agcgttcgcc aggttgagct gcaacacagg attggtgcgc aaatcgcggg catttttcag
    11881 acgattgatc gcctcattgg ctttgttttg cccaagatcg atatcagtag ccagatcgag
    11941 ataccatgcg ttgccaggtt ctgccgccag taacggttgc agcgttttac gcgcctcgtc
    12001 gtatttattg gcttccatcg cctgtaaagc acgaccatat tgtgctgctc gttgctgacg
    12061 aacgtttcct ttcgcccatt catccagcaa atcactggtg agctggttac gtccggaatt
    12121 atacatcccc agtgtgcgcg ctttcgccag atagaaatct tccgacgact gcaccaccat
    12181 cgggcgcatc tgattagcac ggttgcgggc atctgccaga cgactttccg gcaacgggtg
    12241 agtcaataaa atttccggcg gacgcgagga gtaacgcgcc tgatcgagta atttttcgag
    12301 gaaggtcggc atcgcctgcg gatcgaatcc tgagcgttgc agcacctgaa taccaatgcg
    12361 gtccgcttcc tgttcatttt gctgggtgaa actgatcatc ccctgacgcg ttcccgccag
    12421 tgtaccggtc agcgccgcca tccccgcctg cggactggcc atcgccagta aaatagaacc
    12481 taacgcgccg acccaggtca gcggcgcgct gcgctgctga tcttccatcg ctcgcgccag
    12541 gtgacgttgg gtgacgtggg agatttcgtg cgccataact gaagccagtt gactttcgtt
    12601 atcggaataa cggaacaggg cagagtgcag caccacgttg ccgccaaaga aagcaaaggc
    12661 gttaatttcg tcgttgttga tcagaaaaaa atgaaacggt gtcttaaccg aattggcatg
    12721 cgaaaccaga cgcatcccca gcgaattaat atattgcgtt aacagcgggt cattaattaa
    12781 cggcgcgctg ccgcgtagct ggcggacata atagtcgccc atctgcattt cctgaccaat
    12841 ggaaagcgtg cttcctgcgg aggttcccat atccggcaag gtgtctgcgc tgtcggcaaa
    12901 cgccggggct acctgaccaa tggtcatagc agcaatgagg gttgcaacca ggtttttttt
    12961 caactgcctg aacataacct ctatcctgta tttctgaaga tagtcatttg accgatactg
    13021 tgaaatatcg ttcccaccgc gccgcctttg agtgtaacgg cgaagattag cccactcaaa
    13081 tgacttttca gtttcctgaa tccgtcataa aaagcgaagt cttttttgtg acatttcgat
    13141 acaattgcgg atcgcaatcc cgctcttgag gttcagggaa ggtctttatg ctcgaaatgt
    13201 tgatgcaatg gtatcgccgc cgttttagcg acccggaagc gattgccttg ctggttattt
    13261 tagttgccgg atttggcatt atctttttct ttagtggcct gcttgctccg ttgctggtgg
    13321 ctattgtgct ggcctatttg ctggaatggc caaccgtgcg cctgcaatct attggctgct
    13381 cccgccgctg ggcgacgtcg attgtattgg tggttttcgt cggtatattg ctactgatgg
    13441 cgttcgtggt actgcctatc gcctggcaac agggcatcta cttaatccgc gatatgccgg
    13501 ggatgctcaa taagctttct gactttgccg ccacgttgcc gcgccgctat ccggcgttaa
    13561 tggacgcggg cattattgat gcaatggccg aaaatatgcg cagtcggatg ctgaccatgg
    13621 gcgattcggt ggtgaaaatt tccctcgcct cgctggtcgg tttgctgacc atagccgtct
    13681 atctggtgct ggtgccattg atggtcttct tcctgctgaa agacaaagag cagatgctga
    13741 acgccgttcg tcgggtgctg ccgcgcaacc gtggactggc aggacaggtg tggaaggaga
    13801 tgaatcaaca aatcaccaac tatatccgcg gcaaagtgct ggagatgatc gtggtgggga
    13861 tcgccacctg gctggggttc ttgctctttg ggctgaacta ttcgctgctg ctggcggtgc
    13921 tggtcggctt ctcggtactt attccgtaca ttggcgcatt tgtggtgacc attccggtgg
    13981 ttggcgtggc gctattccag tttggtgcag gcacggaatt ctggagctgc ttcgcagtgt
    14041 atctgattat tcaggcgctg gacggcaacc tgttagtacc ggtgttgttc tccgaagcgg
    14101 ttaacctgca tccgctggtg attattttat cggtggtgat cttcggtggt ttgtggggat
    14161 tctggggcgt attcttcgcc attccattgg cgacgctgat caaagccgtg atccacgcct
    14221 ggcccgatgg gcaaatagcg caagaataat cttcagcccg acgaactgtt tcgccgggct
    14281 gatttttcag gattcctgac gtaaataaat agcccgataa cacatactca ccagcactgc
    14341 accgccgata atattaccca gcatcaccgg gagcagattg gcggtaataa aatggcttac
    14401 cgtcagtgcc ggaaaattgt ctgcgctact gtgcgccagt tgccagaagg aggtaggggc
    14461 gaaatggcga atggcaatgg cgaacggaat cacaaacaaa ttggcgatac agtgctcaaa
    14521 gccactggcg acaaacaggg tgatgggcaa aatcatggcg acgattttgt cgcataacga
    14581 acgcccgcaa taactcatcc acagcgccag gcaaaccatc agattgcaca taatgccgag
    14641 gctgacagat tcagtaaatg tatgatgcat tttgccctcg gcgcagtgta aaaccgcgac
    14701 tccccacatc gcgttttcac tcatcaccag cccggaaaac cagattaaca aactgaaaca
    14761 ggcaatacct gccagattac cgcaggccac cagaagtgcg ttaatcagcc aagttcgcca
    14821 actaataacg ccccgacttt ttgccatcac cgtcattacc gacgaggtga acaggctggt
    14881 gccgcaaacc gccagcaaaa taaagccgag tgtaaagcaa aggccgccca ccagatgggt
    14941 taatgcctgt gaagacgggg catcggcgat aacggaaagg taaaaagtaa atccgatcgc
    15001 cataaacacg ccagcggaca tcgccattaa aaagaccgac actttactgc gggcaatttt
    15061 atgggcggca atccgttcag cgattgccgc ttttctggcg ctcaactgca agtcgaaaga
    15121 gagtttgttt ctcatgcata ctcctgcaaa agcagattac aacacctcgc gaaccgagat
    15181 ccccagacgc tgcattcgtg acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc
    15241 gccacggggc ccggcaacaa tgccattggt ttctcgcaat acctgaacaa tgcgctgacg
    15301 ttcttcttcg tcattctctg gcgtcgtcgg gtgcagtaac tgcgccattg aactgcgaag
    15361 cgctgaatct tcatttagcg tcggcagtaa acggctttgt cggacattaa gatgtaaatt
    15421 cagactgtta ccgcgagtca gtagtaccgc tcgctcaatc acgttttcca gctcgcgcac
    15481 gttgcctggc cagtcccacg acatcaactg gcgtagcgcc tcggtcggga tggcgtcaat
    15541 tgcgcgattc atatggcgcg ccattttttg cgtgaaatgc tttgctaaaa gagggatatc
    15601 ttccggacgg tcgcgcagtg gcggcagttc cagtgggaag acattcaggc gataaaagag
    15661 atcgctgcga aactggcgat cttcaaccat ttgccacaaa tcacggttgg tggcggcaat
    15721 gactctgaca tttaccggga tcgttctact cccgccgaga cgctcaattt cccgttcctg
    15781 caatacgcgc agcagtttag gctgaagttc taacggcaga tcgccaattt catcgagaaa
    15841 caacgtgccg ccatcggcaa tttcaaaacg accacgatgg gtattaatcg caccagtaaa
    15901 tgcccctttg tcatgaccga ataactcact ttccagaaga ctggcgggga tggcagcgca
    15961 gttgattttg accagcggct tgtcgcgtcg tgggctaagt tgatggatcg ctctggcgat
    16021 cacctctttg ccggttccgg tttcaccaca aatcaacacc gtactgtcgc tcttcgccac
    16081 aatatctacc tgctggagca aatcttccat tgcctggctt tgatagataa tgtcaccgac
    16141 gcccagattc gaaaggagct gctcgctaag ctggtggttt tcttgctgca agctttcctg
    16201 caaatcggtc atgctgcgcc aggcatcggc attgccaacg gcaatagcga tgcgatcggc
    16261 aatatgttgt agtagctggc agttttcctc actaaagaga gtggaagatg tatgcgccag
    16321 caacaatgca cccggtgtat ggttgccaaa ggtgagcggt ataaggagcg cagattcgca
    16381 gccgttagat gcaagtaagt gtaataacgg gtcgcgttgc cagagaaaca gatcgtctgc
    16441 acggtgcgtc aaggtcggtt gtcgggtttg tagcgtttgt gtcagcaata cactttcgcc
    16501 aggcatattg cgtggcagac atgcacaatc tgaggcggaa aaaacgctgc accacaggct
    16561 gaacttctcg ttctttcgat gatcgcccag taccatacag actgaagcca gaccgaaaaa
    16621 atgatggatc tcatgagcga cgtcagcgat cagatcgtcg agatcaagat gtgacaacac
    16681 tgcattagtg atatccacca gtacccgatg atgatcgcga tctttctcca gcaaacgcag
    16741 cgccacctgc tgttcttcat cattgagcca tgattgcaga accggggtga ttatccgcgc
    16801 cagccagtta aacagtgtgc aatcctgggg agtaaaacgg ctgccgtgag tgcgaataaa
    16861 gcgtagctcc gccattccac tactgccgag aaggacggcg tgacgcgaat aatcgaccat
    16921 ctcgccctga tactctgttg ttgccgtcac atcgctgcca tcatcatggt agcggtaata
    16981 gaagttgctt ttgagaacat gcagttcaat aagttgtacc gggctgaacg gcggcggcag
    17041 cgggatgaag gcgcgtaata aagacgcctt gccgtgtttc tgcgctaacc gctccgcgag
    17101 cattccgttt accgcttcaa ttgttattcc ttgcggcggg gcaaacatcg cctcgtctga
    17161 catagccatc aacagtcctt attaatgaga aatccttctc acgcaatttt tctggccatc
    17221 agatagatgg ccggttcctg cacaatttca tccaacagat gaatgaattg aatgctacgc
    17281 gcctgccacg ccgacgtcgc gtcatctgcc agtggcgcga atgcccgggt caacagggcg
    17341 gtgtggctgg cgtcaataac aatctcacca aaggtcatca cccccagcat cttacgtcgg
    17401 gcttgcttct cttcgacctg tgcaagccaa tcttcgtatt ccgtctgtgg gcagcggaag
    17461 gcgacattca gacaatcaat cacgccaacg tgatgaccga tagccaacga gtaatacatt
    17521 acctgggagc tgtgttccgg catctcgtcg ctactggcga taaatttttt tcgcagcgtc
    17581 cagaaaacaa tttccccgca ctcttcagtc atgtaacccc cggagtacgc gaaagagttg
    17641 ctgaacgata ctattgagac gtggatcgtc ggcatcgcgc aaccaggtgt taacccgatt
    17701 tcctgtagga tcgtcgctta aatggcgcag gagccgatcg caaatttctc gcccctgacg
    17761 atagcccgcc agccgccgcg cttctcgctc aatggcgata cgctgtgatg gcgggatctg
    17821 cggccacaac ggttgcatag tcaccccggt gggatcgcga taatccaccg cgtgaatctt
    17881 ctgttgcagc aaaccgagcg ccacggcgaa accgtgaatg gtggcggccg gtgttggcgg
    17941 gcagccgggg atccaaacat caatggggac aatggtgtcg ctgccgcccc agacgctgta
    18001 gagatcgtgg aaaataccgc cgccgacacc gcacgcgccg taggaaacac agattttatg
    18061 atcgggggca gactcatacg cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc
    18121 agtaaataac aaaatatcgg cgtgacgcgg tgatgaaaca accttaatgc caaaacgttc
    18181 tgcgtcgaat actggtgtga tggcggcaaa aatttcgatt tcacaggcgt tgcagccgcc
    18241 gcaatcgacg cgataaacgt aggctgagcg acggatatcc tgtagcaaat gccgcttcat
    18301 cttttgcgtt tgctcgtcca gcgtgatggg ctggctgaca tgttgtgtaa gcactggact
    18361 catagctgct ccttagccac cagcggtaca tctgtatcgt cgttgatcag cgtcgcgcgt
    18421 tgtttgcact ccgggcagac gctcgcttgc gcccgcaaca tttcgcggtt ttgtggcgca
    18481 ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg ttttttgcgg ggcaaacggg
    18541 cgttcgcaac ggctgcaacg ttgtagatgg aacgttgcgc gggtatagag atcggcttta
    18601 ttggtgacgg tcagttcaaa gttattggta agctggatgg ctctggtcgg gcacacttct
    18661 tcacaacgtc cgcagtaaat acaacgcccc agatagagct gccaggtgcg cgaattttgc
    18721 tggtcgtcgg tctggatagt cagcgcattt gccggacaag cacaggcgca ggcaccgcag
    18781 gcaatacatt ggctgggcat caggtccggt ttcccgcgaa agcctgggct gacctccagt
    18841 ggcgcgaagg gatatttcac cgtcgcggtt ccggcgcgca taatagtttt cagtaacttc
    18901 agcatcttct gtccttactt cagcggcgaa cggttacgat caatgccgta gcgttcgatc
    18961 tctttatacg gcacggtttt tgactggcgc ttgcgcacat caaccagcgt cacacggtcg
    19021 gtacaggagt agcagggatc aaggctaccg ataatcagcg gtgcgtcaga aacggtattg
    19081 ccgcgtagca tgtaacgcag caccggccag ttggcgtagg tggcggcacg acagcgccag
    19141 cggaacaatt tttggttatc accgagcatg ctccagtgca cgtcttcacc gcgtggcgct
    19201 tcaacaaagc cgagcgcgaa tgcgtgaggt ttatagctaa agccttcggt cagcagcggg
    19261 gtatccggca tgttgtcgag ggcaaattcc agcattgcca gcgaatcaaa cgtctctttg
    19321 acacggacca tcacgcggga gaaaacatcg ccgccggtga aggtaaacag tgtttttgga
    19381 atattgccgt agtcggcgta ggggtgatca aagcgcaaat cacgggcaaa accactgccg
    19441 cggatcagcg gccctacagg gctataatca cgggcgattt gtcggtcgag aatgccaatg
    19501 ccctgagtgc gttgttccat atttggcgtg gcaagcagca tttctaccag ctccgacacg
    19561 tcggcgcgca tctcgcgcac cagtttcagc gtttgcagac gttgctcttt gagaatatcg
    19621 cggcgaacac caccaatcag attcagaccg taggttttac gcgacccggt cagcaattcc
    19681 gccattgtca tcgacttttc ccgcacgcga aaaaattgca taaagccggt atcaaaacca
    19741 acaaaatggc aggagaggcc aaggttgagc aaatgactgt gtagccgttc gacttccagc
    19801 agaatcgagc gaatggtatg cgctcgttgc ggcacctcaa tcctcagtgc attttcaacc
    19861 gagttggtat aggccacact gtgggcaaaa ccgcaaatcc cacacacgcg gtccgataag
    19921 aaggtcactt cgttgtagcc catccgcgtt tctgccagtt tctccatgcc gcgatggaca
    19981 taaaacaggc ggtaatcagc atcgacaatt tgctcgccat ccacaaacaa gcggaagtga
    20041 cccggttcat cggaggtgat atgcagcggg ccgacaggga tcacccgcgc atcgctgttg
    20101 ccctcattga taaacggata cgtttcggta tcagtcgtct gttcagggcg cagtcgataa
    20161 tccatcgcat ctttgcgtag cggatgcata tcttccggcc agtcatcggg caacaccagg
    20221 cgacgctgat ccggcaggcc aaccggaatc agcccgtaca tatcgcgaat ttctcgctcg
    20281 ccccagaccg cggcagggac gcgcggtgtg actgacggaa actcccgact gtcggcatcg
    20341 accagtgcct tcaccacgat ccagcatttt tcggcccctt ccatagaaag ggcataataa
    20401 accgcgtaat gaccgttaag tgtccgctcg tcgttgccaa acaggaccgg aagccagcca
    20461 tcatgttgat aataaagata atgtacaacg tcaggcagca gattgatttt caccgtaatg
    20521 gtgacctgtt caggcgtttg tcgctcttca tccagcaccg cgccggggaa ctgcgttttc
    20581 agggcggcga gaatcgcttc gccacgattt gacgatgaat taacgttcac tgtatttctc
    20641 cgaaacagat gcggtgcctg aaggcaaaaa atcatgccag gtgctacgtt gtgcaggcag
    20701 gtcgtgcgtc cctgagagga ctatagtgga agcgcccgcc aggatcctga tgacaggttg
    20761 tggaatatgc gttcccatcg ccagcatcat gaccagcaga atcaccattg gcgaggtggt
    20821 caaccagccg agatcacctc ggttaacggc ctgcggcggt ttcgccatta acacccgcgc
    20881 agccatccgt accaggcccg ccagcaccag cgttaacagc aataacagca ggacgataat
    20941 cagcaggtga ttacgtgcca gtccggcggt aacggtcata aattcgctaa gaaaaatgtt
    21001 gaagggcggc atccctgcca gcgccagcgc accgccgcca aacagcacgg cggtaaatgg
    21061 catgattttg agcatcccac agacgacgtt gagatcgcgc gtgccgtact tgagcagtac
    21121 attgccggaa ccgcagaaca gcagcgtttt tgccagactg tggtttaagg tgtgcagcag
    21181 cgcggcaaaa attcccagcg gcccgccaat gcctagcgcc accgcgacca gtcccatgtt
    21241 ctccacgctg gagtacgcca gcagacgctt aatgtcccgc tgtaccagaa tgaaaaatgc
    21301 cgccacggca accgacaaca tgccgaagat gagcaacaac cggttgggga aatcgctgcc
    21361 gatggcctgg caaataatga tgtaatagcg aatcagcacc aacagcgcgc agttcagcaa
    21421 tacggcggag agtagggcgc tgaccggact cggcgcttca ctgtgagcat ccggcagcca
    21481 ggcgtgcatg ggaaatagcc cggttttggt accaaagcca attagcacaa acacaaaggc
    21541 caacagcatt aatgttgggt caagcaagga cgattgctta agaacctcgc tccagaatat
    21601 cgccatttct gcctgcggca taacgctggc ggcgttggcg tataccagca cggtaccgaa
    21661 cagaccaaaa gcgacaccaa cagtacaaat aatgatgtat ttccatgcag cttccagcga
    21721 tgaacgctga ccgtaaatgc ctaccagaaa cgccgagctt aaggtggtgg cttcgatcgc
    21781 cgcccacatc acaatcaggt tattgctggt aacaaccagc agcatggtga acaaaaacag
    21841 atggaagaaa ccgtagtaat cgcacagcgt aacgggtgaa agctcgccgt gtgccacttc
    21901 atgacgcatg tagccaatcg agtaaacacc ggtgagaaag ccaatcacac caagaatggc
    21961 gaggaacaaa ccgcccagac catcaatatg aagccacagt cccgcagcga atatttctcc
    22021 tgcatcagcg gcagtttgga ccacccagag tgccagaatc agcagcagtg tgatccctaa
    22081 gcaatgtaat acggtcaccg tgcgagtcgc agagtgcccc cgtttccggc aggcaaaaca
    22141 gagcagcgaa aaaagcagcg gcgtgagcag gagtaaagcg aacatcacag aataactcat
    22201 ctcattatcc cttcagcgcg gtcaagttgt tcacgtccag cgtgccgtgg gtacgccata
    22261 tttttcttgc cagtaacacc atcacaatga cggcgaagat ggcgtcggta gcgataccaa
    22321 tttccaccag ttccggtgca cgccaggcta gaagcgccag caccagatgg gagccgtttt
    22381 ccatcaggca gtaaccaaaa atttgccgca ggatattgcg ctggctgaca atgcacagca
    22441 ggccaagcag aaaatgacct aacgctaccg ccagcgccgg tttcagcccg gtagccatcg
    22501 gtagcttcac gggctgaacg acaaatgcgc aaagcaggac aattaacgcc gcgagcagtg
    22561 ccatcattgc cggaccgaat aacgcttttt ccgggatgtt ctggggaata tttcgtgcag
    22621 cgtaagtcat gattaacggt accagcagca ctttggtgat aaaggcgctg gcggaccaga
    22681 tcagcagttg ctctgcggcg aacaggcacg agagggtggc gaaaatagac accagcacca
    22741 gcgactggca ggcgtaaaat ccgcatgaca ggcgatagct tttgacgcta atcacaaaca
    22801 gcgatgtcag catcatcagt cccgccagat tatttacgat cgtagaaccg gtcattcatc
    22861 atctccttac tgcaaccagg tcgcggcgat tacgctggaa atcagcgaca tcacaatcag
    22921 tacaatcaac accaggcgca ttgatcctgg cagcggtgcg gcatcggcga cggcctcgct
    22981 cggtttgcca ggcacgacgc gaccaaacca gcgaataaac caggcgaaac aagcgaccga
    23041 ttcaatcatc agcagaatca gggcgggcag caggatccag tactccactg acaacgcaaa
    23101 accggcagca aacagcggga atttactgaa gaagccgttg aacggcggta cgccggtaat
    23161 cgccagcgct gccacgcaga aacccacgcc tggcaacggc agggtgtgca atacgccacg
    23221 cagacgcggc aacaagcgcg tgccgcagct gtaactcagc gcacctgcta caaggaaaaa
    23281 caggctttta gcgaacgcgt ggttgacgat gtaggcgatg ctgccctcca gcgccagccg
    23341 cgagccgaag atggagagcg acaaaccgaa gaacatccag ccaagttgag tgatggtcga
    23401 ccaggccagc aaccgcttca tatcctgctg tggcaaatac atcagaaatc cataaagaat
    23461 ggtgaccagg gccatgacca tgccaacgcc gccaatcaca tgcgggatat tgccgccgtc
    23521 gataatggcg cgggcaaaaa tgtaaacgcc cactttcacc atcgatgcgg cgtggagata
    23581 ggcgctgatc ggtgttggcg cttccattgc gtccggtagc cacgcttgca tcggtagctg
    23641 ggccgatttc ccccacgcgg caaacaggat gccgccataa accagataac gtgcgtcgcc
    23701 gtgtaactcg ctcatcgcgc taagcgcaaa cgttccggtc tgcaaaaaca gcgtggcggc
    23761 ggcaagatac aaccccaacg aaccgatatg agtgataagc aacgctttta gtgccgaacg
    23821 ctgcgcttta tcgctctggt aataactgat caacgcccag gagcagccgc ccgtaatttc
    23881 aaaaaacaac aactgaccga gcagcgtcga ggagagtacc agtcccgcca tcgcgccgat
    23941 aaacaccagc aaaaatgcgt aataacgatt cgtgccgtta tgcgggtgtt cgcgattttt
    24001 atccgtcaga taacccgtgg agtagatcgt gaccagcaaa ccgagaaaca ccaccacaaa
    24061 cagaatcagc gtactcacgc gatcaatgac caggccaaac aatgccacat cccccacgtt
    24121 gaccaacgtc aacgtgacgg caactttatc ggcctgataa aacgcggaga tcagcgacaa
    24181 catgcacagc gtggtcagcg cggcgaacaa aaccccccat tcggcggccc gacgttgtgg
    24241 cgaaaacgaa acgaccagtg cgccgataaa aggcagcaat aacgtcgtca gagcaagatt
    24301 ttccatattc cgtcagtgct ccttacagac cggttaacca gaagacccat gcaagcgcag
    24361 caaggctgaa gccaagccag gtcacatggt gaatgagtaa aaaacgcccg cgtgccagcg
    24421 tgttttctgc gatagaggcc agaacaaaaa tcagcaaaac cttgagcagc gtaacgacaa
    24481 gtgaagtcag caggcaggcg agagaaagct cttgcgcgcg cccaaagggc agaaacaggg
    24541 ccacaaacag tgctgccatc acgacctgtt tcagccccag cccccatttc gctagcgcca
    24601 gcccggcacc ggaatattcg gtcagcgggc cttcctgtaa ttcctgttct gcttcagcaa
    24661 catcaaaggg aatttttccc atctcaatga agcaggcaaa accacaggcc agtaacgcca
    24721 gtacggtggt tagcggagag ttccagcccg ttgccagcgt attgctgatc atctcgatat
    24781 gcgtggaacc tgctatcagc gccaatacca gcagtgagag aatgagcatg ggttcgacca
    24841 gaatgccgag cgtcaactcg cgactggcac cgactcccgc aaacgggctt ccggtatcca
    24901 gcccggaaag agcaaagaaa aaacgaaaca gggcaagaag atagataagg gtgatcagat
    24961 cgccgccgcc cgcaaaaggg gaaacggtaa taaatagtgg tagggccatc gccagcacca
    25021 gcatgctgct gattaatacc cacggcatca ggcggaacat cagacctgaa gatgtcggcg
    25081 caacttcctg gcgtttaaac agtttgtgga tatcgcgata atcctgccag atccccggtc
    25141 cgcggcggga gtgcatacgc gcgcgtatct gccgggaaat acccgtaaaa agcggggtta
    25201 gcatcagcag aatcacggcc tgtgctaacg caaaaatgac cagatatccg tcgcaaagag
    25261 tttgtctcat ggtgatttcc ttagacggca atagcgatta gcagcacaac cagtgcggca
    25321 accacataca ggcaatagag acgaaagtcg ccgctttgca gatgctgtat ttctttggcc
    25381 agccgttggg tggcgctcac gatggggcgg atcacccgct catcccagaa gggttctgtg
    25441 ctctgagccc tggcggtgac gtgcgcaaga cctttattta gcctcagcgt agggtcgagc
    25501 tgttgtcgta gacgaaatag cgcagaaaag accacacgca gcggctgcat cacgccattg
    25561 cctgacgggg ccatcgcatt ttcccagccg tagccgcatg cccaggcatc tcctgtgcga
    25621 cgaaacgctg cgcggcgcga acgacaccac agccagtaaa ggccaggcag caaaggcatc
    25681 gccagtagta acagcaacag taatgaggga gtgacccgtg tatgaaacgt gccgggtaca
    25741 agtgctattc cgctggcgac agtgacggga ggggtatcgg taaaggcatg ggcaatatgc
    25801 attatcttcg gtgccagcca actggcacta agcgcaatta atacgcagag tgccgcgagc
    25861 agtagcatgg cgacgatcat cgtatttggc acttcctgtg cctcttcagc gtgtgtactg
    25921 cgcggcgcac cacagaaagt aataccgtac attttgacga agcacattac tgccagccca
    25981 ccagtgactg ccagcatcac catagcaata ggacccgcaa gttgtagcgc tacggcttcc
    26041 acacgactta gtgagaacag cgactgccag gtgtaccatt cgctgataaa accattcagc
    26101 ggaggaaggg ctgatatcgc gaggcaacca atcaggcatg ctgcggctgt ccacggcatc
    26161 cgttttgcca gtgcccccat tttttccatg tcgtgggtat gcaaacgcga aataatcgca
    26221 cccgcgccga gaaatagcag ccctttgaac agcgcatggt tgagcagatg aaacagtgcg
    26281 ccgagcagtc caaccacggt gagcagcggg tcgtgcagtg acagaccgac catcgccaca
    26341 ccgactgcca gcaaaataat gccgacgttt tcgacggtac tccaggccag cagccgtttg
    26401 atatcctgtt ccgccagcgc atatagcacg cccaggagcg cggagattgc gccgatcgcc
    26461 atcaccagaa tgccccacca cagaggcaaa cccgtttgcg ccagcagatc catcgctact
    26521 ttcaggatgc cgaaaatacc gattttgacc attacgccag acatcaacgc cgaagcgtgc
    26581 gatggtgcgg cagggtgagc gcgcggcaac cagctgtgca acggcatcat cccggctttc
    26641 gcgccaaaac cgaaaaaggc cagcaggaac accgccgacg ccagccccgg agaaagtgaa
    26701 agcgtgcgga aactggcaaa atctaggctg ccgctttcgc gccccatcag caagaaggcg
    26761 atcataatca gcaccgatcc ggcgtgggcg ataaaaaagt agagcatgcc agcgttgatc
    26821 gacgttttat cctgcctggc aatgaccaga aaccaggaag acagcgacat catttcaaac
    26881 agcacgatga accaaaaagc gttgtccatc accagcaggg caaccatcga tgcgatgaaa
    26941 atattcataa agaagcccat cgccgccgcg cctttgccct cgtattcgcg catataagtc
    27001 aatgaataga gcgaacaaac caccaccagc aaggagataa ccagcaccat aaatgccgag
    27061 aggctatcca ttcgcagggt taaatcggca aagtcaaaag gggtggcaaa acgggcgact
    27121 aaagttacgc ctgcatgtaa ttgcgtaatt ccgctgatga tgccaatcac gccgcccacc
    27181 agcgatgtga tgccggaaag cttaatagcc agtctgtcca gaccgagtaa aaacagcgaa
    27241 gccagactgg caaacagata gaggatcagc gaccaggtta ataattgcag ggcatccatt
    27301 agcgttgctc ctgagtgagg gaaaggggat cctccccgcc attggcaaac cagcttgcgg
    27361 caaggcgctg tttttctttc atctgacgtt gcaggctatc gccggtgatc agccgtaacg
    27421 cctgattcgg gcaagcgcga acgcaggccg gaccttctgg caagaaatca cacaggtcgc
    27481 atttcaccgc gacggtctgg acacccggtt cccagcgcag caaagcatgt tgtgttggcg
    27541 cgttttcttc gccgtctttg agtgagcctt cagcctgaaa aacatattgc gcaggggcat
    27601 tcaccggacg gcttcctgaa gcgctgattg cgccaaatgg gcagaccacg gcgcaaagct
    27661 tgcagccaat acagaggctt tcgttgagtt ggatcgcatc atccctctgg gagatggcat
    27721 tgaccgggca gacctgcagg caaggggctt cctcacagtg atgacacacg acaggggcag
    27781 tgagtgttga cgtcttcgcc agggccaggc gcgggtgttg ctgtaaacct tgcgttttat
    27841 gcacgtccga acaggcagcg agacaggtat tacatcctgt acaccacagt ggttcggcca
    27901 ccacaaagcg gttcatgtcg gctccttgaa aatgatatga aacattgcat ttcatattgc
    27961 ataaaggatg ccagtctgat taatttaata tattcaatga gttatgaatt aactggattc
    28021 tgaacggtac aaaaatgatg gtcattaacg ttcagcaaaa aatggattcg acacccgtgt
    28081 cgcaggggaa agcgagaatg tgatcttgat cacgataagt gaagtgtgct gaccgcaagc
    28141 gcagccagca cggaatggag caaagtaatc aggcgtgttc tttcagccag ttcagcacaa
    28201 cgtcgtggtg attgctggtt ttgaaatcgt caaagacatg ttcgattttg ccatcagcgt
    28261 caatcaggaa gctgatgcga tgaataccat cgtaggtttt gcccatgaag gacttttccc
    28321 cccagacgcc gaattgctcg cacacctggt ggtcctcatc agacaggagc gtaaagttaa
    28381 gcagctcttt ttccgcaaaa cgggagagtt tttcgggttt atcggtgctg atacccagca
    28441 catcaacgcc cgcttttttc aactcatcca tgttatcgcg taagccgcag gcctgtacgg
    28501 tacagccggg ggtcatggct ttcgggtaga aataaaccag aacacgctgt ccctggaagt
    28561 cggtcaaatt aacttgttct ccgtcttgat ccggcaagct aaatttcggt gcgatatcac
    28621 cggctttcag tggattcatt acttaactcc atcctgttca tcatgttggg aataattgac
    28681 gacgttaata ctgccttgtg cattgagttc tgtacatagg gctttgaacg cttgctcaat
    28741 atttgctgcg tccgcagatg cggggctgtg ggcggttatc tgaatatgca actgcgcagc
    28801 ccgttcattt tcagcaggtt gtgtgcgcga caccagctcc gcaatgttca tatgatgcgc
    28861 gtcgaaaagt gctgtgaagc gttcaattaa atgcggggag tctgcaacat cgacctgaac
    28921 ccagacagat gctggcattg gcggacgcgg acgcgccgtc gtgcgcttca tcacgattaa
    28981 aagatccagt tcggcacctt tcaacggtaa cgttgattca atcagagtga tggcattcca
    29041 tgaaccggaa agcagcatga taaacgtgaa ctcttctccc agcatcgcca ggcgactgtc
    29101 ttcaatatta cagccgcaac tactgacatg acgggtgatg gtgttcacaa ttccagggcg
    29161 atcggcaccc aacgcagtga tcaccagata atgttgcgat gacagtgtca aactggttat
    29221 tcctttaagg ggtgagttgt tcttaaggaa agcataaaaa aaacatgcat acaacaatca
    29281 gaacggttct gtctgcttgc ttttaatgcc ataccaaacg taccattgag acacttgttt
    29341 gcacagagga tggcccatgt tcacgggaag tattgtcgcg attgttactc cgatggatga
    29401 aaaaggtaat gtctgtcggg ctagcttgaa aaaactgatt gattatcatg tcgccagcgg
    29461 tacttcggcg atcgtttctg ttggcaccac tggcgagtcc gctaccttaa atcatgacga
    29521 acatgctgat gtggtgatga tgacgctgga gctggctgac gggcgtattc cggtgattgc
    29581 cgggactggt gctaacgcta ctgcggaagc cattagcctg acgcagcgct tcaatgacag
    29641 tggtatcgtc ggctgcctga cggtaacccc ttactacaat cgtccgtcgc aagaaggttt
    29701 gtatcagcat ttcaaagcca tcgctgagca tactgacctg ccgcaaattc tgtataatgt
    29761 gccgtcccgt actggctgcg atctgctccc ggaaacggtg ggccgtctgg cgaaagtaaa
    29821 aaatattatc ggaatcaaag aggcaacagg gaacttaacg cgtgtaaacc agatcaaaga
    29881 gctggtttca gatgattttg ttctgctgag cggcgatgat gcgagcgcgc tggacttcat
    29941 gcaattaggc ggtcatgggg ttatttccgt tacggctaac gtcgcagcgc gtgatatggc
    30001 ccagatgtgc aaactggcag cagaagggca ttttgccgag gcacgcgtta ttaatcagcg
    30061 tctgatgcca ttacacaaca aactatttgt cgaacccaat ccaatcccgg tgaaatgggc
    30121 atgtaaggaa ctgggtcttg tggcgaccga tacgctgcgc ctgccaatga caccaatcac
    30181 cgacagtggt cgtgagacgg tcagagcggc gcttaagcat gccggtttgc tgtaaagttt
    30241 agggagattt gatggcttac tctgttcaaa agtcgcgcct ggcaaaggtt gcgggtgttt
    30301 cgcttgtttt attactcgct gcctgtagtt ctgactcacg ctataagcgt caggtcagtg
    30361 gtgatgaagc ctacctggaa gcggcaccgc ttgcggagct tcatgccccg gctggaatga
    30421 ttttgccggt gacctccggt gattatgcaa tcccggtgac caacggtagt ggtgctgtcg
    30481 gtaaggcgct ggacattcgt ccaccagccc agccgctggc actggtttct ggcgcgcgta
    30541 cccagttcac gggcgatacc gcttcattgc tggtggaaaa tggtcgtggc aatactctgt
    30601 ggccgcaggt ggttagcgtg ctgcaggcga aaaactacac catcacccaa cgtgatgatg
    30661 ctggtcagac actgaccacc gattgggtac aatggaaccg tctggacgaa gacgagcagt
    30721 atcgtggtcg ttatcaaatc tctgttaagc cgcagggtta tcagcaggcg gttacggtta
    30781 aactgttgaa cctggaacag gcgggcaaac cggttgcaga cgcggcttcc atgcagcgtt
    30841 acagcacgga gatgatgaac gttatttccg ccggtctgga taaatctgcc actgacgccg
    30901 cgaacgctgc gcaaaatcgt gcctccacca ctatggacgt acaaagtgca gctgatgaca
    30961 ccggtttacc aatgctggtc gtacgcgggc cgttcaatgt ggtttggcaa cgtctgccag
    31021 cggcgctgga aaaagtgggc atgaaagtga ccgacagcac ccgttcgcag ggcaacatgg
    31081 ccgtaactta taagccgctg tctgacagcg actggcagga actgggcgcg agcgatccag
    31141 gcctggcatc cggtgactat aaactgcagg ttggcgattt agataaccgc agcagcctac
    31201 agttcatcga tccgaaaggt catactctga ctcagagtca gaacgacgcg ctggtagctg
    31261 tcttccaggc tgcgtttagc aagtaaaaat acagggctgg aatcatccgg cccttttttc
    31321 tgatatgata cgcaaacgtg tgcgtcggca ggaaaacgcg attttagcgg taattcgcac
    31381 gaaatttgtt tgtcggacgt agttcggata aggcgttcac gccgcatccg acaaaacatc
    31441 cggcacacca gatagcaaaa gattttaaaa cgttaattca cacccaggag tgataaagat
    31501 gcaaaagcaa gctgagttgt atcgtggtaa agcgaaaacc gtatacagca cggaaaaccc
    31561 ggacctgttg gtgctcgaat tccgcaatga tacgtcagca ggggatggcg cgcgcattga
    31621 gcagtttgaa cgcaaaggta tggtgaacaa caagttcaac tacttcatta tgagcaaact
    31681 ggctgaagcg ggtatcccga ctcaaatgga gcgtctgctc tccgataccg aatgtctggt
    31741 gaaaaagctg gatatggtac cggttgagtg tgtcgtgcgt aaccgtgctg cgggctctct
    31801 ggtgaaacgt cttggaatcg aagaaggtat tgagctgaac ccgccgctgt ttgatctgtt
    31861 cctgaaaaac gacgccatgc acgatccgat ggtcaacgaa tcttactgcg aaacctttgg
    31921 ctgggtgagc aaagagaacc tggcgcgtat gaaagagctg acctacaaag cgaacgacgt
    31981 gctgaaaaaa ctgttcgatg atgctggtct gattctggtc gacttcaagc tggaatttgg
    32041 tctgtacaaa ggcgaagtgg tactgggtga tgagttctcc ccggacggta gccgcctgtg
    32101 ggacaaagaa acgctggaga aaatggacaa agaccgtttc cgccagagcc tcggtggcct
    32161 gatcgaggcc tatgaagccg tcgcccgccg cctgggtgta cagctggact gatttttctg
    32221 ttcatcatct tgccgtgctg ctggcacggc aagacaacca ttctcgtaag atgtgcattg
    32281 aacgctcatt cctccgccat ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc
    32341 tacgatcatt tctataatga atatattgtt gaggtatcta tgcgttggca agggcgacgt
    32401 gaaagtgaca atgttgaaga caggcgcaat agctccggcg gtccgtcaat gggcggtccc
    32461 ggtttccgtc tgccgagcgg taaaggcggg ctgattttac tgatagtcgt gctggttgca
    32521 ggctactatg gcgttgattt aaccgggttg atgaccgggc agccggtttc tcaacagcaa
    32581 tcaacgcggt caattagccc aaatgaagac gaagccgcaa aattcacctc ggtgattctg
    32641 gcaaccacgg aagacacctg gggacaacag ttcgagaaga tgggtaagac ctatcagcaa
    32701 ccgaagctgg tcatgtaccg tggaatgacg cgtaccggat gcggggcggg ccagtccata
    32761 atggggccgt tctattgccc ggcggatggc acggtttata tcgatctctc cttctatgat
    32821 gacatgaaag acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgcccat
    32881 gaagtcggtc atcatgtgca gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa
    32941 caaaacgcga cgcaggcgga agtgaaccgc ttatctgtgc gtatggaact ccaggccgac
    33001 tgttttgccg gtgtctgggg gcatagtatg cagcagcaag gcgttctgga aaccggcgat
    33061 ctggaagagg cgctgaacgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt
    33121 caggggcgag tagtaccaga cagtttcact catggcactt ctcagcaacg ctacagctgg
    33181 tttaaacgtg gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt
    33241 taacttccgg ggcagggatg gctgaactga ctgcgcttca cacattaaca gcgcaaatga
    33301 aacgtgaagg gatccgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc
    33361 atgcgcttaa gttacgtgat gccttacctg gcgactggct gtggatttcg ccgcagccag
    33421 atgctgaaaa ccactgttct ccctcggcac tacaaacttt acttgggcgc gagttccggc
    33481 atgcggtatt cgacgcccgc cacggctttg atgccgctgc ctttgccgca cttagcggaa
    33541 cgttgaaagc gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc
    33601 aacctgatgc cgactcgctg cgctggagtg attgccctga ccctattgcg acgccgcatt
    33661 ttgtccagca tttcaaacgc gtacttacgg cggataacga cgctatcctc tggcggcaaa
    33721 accagccgtt ctcgttggcg cattttactc cccgtactga ctggcacccc gcgaccggcg
    33781 caccacaacc agaacaacag caactcttac agcagctact gaccatgccg tcgggcgtgg
    33841 cagcggtaac tgctgcgcgt gggcgcggta aatcggcgct ggcagggcaa ctcatttctc
    33901 gtattgcggg tagtgcgatt gtcactgcgc ccgcaaaagc ggcaacggat gtactggcac
    33961 aatttgcggg cgagaagttt cgctttattg cgccggatgc cttgttagcc agcgatgagc
    34021 aagccgactg gctggtggtc gatgaagctg cagccatacc tgcgccgttg ttgcatcaac
    34081 tggtatcgcg ttttcctcga acgttgttaa ccacaacggt gcagggctac gaaggcaccg
    34141 gacgtggttt tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactgc
    34201 aacaaccgat ccgctgggcg cagggatgcc cgctggagaa aatggtcagc gaggcactgg
    34261 tttttggcga tgaaaacttc acccatacac cacaaggcaa tatcgtcatt tccgcatttg
    34321 aacagacgtt atggcgaatc gagccagaaa cgccgttaaa ggtttatcag ttattgtctg
    34381 gtgcgcacta ccggacttcg ccactggatt tacgccgcat gatggatgca ccagggcaac
    34441 attttttaca ggcggctggc gaaaacgaga ttgccggagc gctgtggctg gtggatgagg
    34501 ggggattatc tcaagaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta
    34561 atctggtggc ccagtcgctg gcggcgcacg gcagcaatcc actggcagcg acattgcgcg
    34621 gacgacgtgt cagccggata gcagttcatc cggcgcgtca gcgggaaggc actggtcagc
    34681 aactgattgc tggtgctctg caatatacgc atgacctcga ttatctttcg gtgagttttg
    34741 gttacaccgg ggagttatgg cgtttctggc aacgctgcgg ttttgtgctg gtgcgaatgg
    34801 gtaatcatcg tgaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagtg
    34861 atgcgggtaa acagctggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc
    34921 tcgcgcaatg gaatggcgaa acgctccctg ttgatccact aaacgatatc gtgctttctg
    34981 acgacgactg gcttgaactg gctggttttg ctttcgctca tcgtccgcta ttaacatcgt
    35041 taggttgctt attgcgtctg ctacaaacca gtgaactggc attaccggcg ctgcgtgggc
    35101 gtttacagca aaacgccagt gacgcgcagt tatgtacctc acttaaactt tcaggccgta
    35161 agctgttact ggttcgccag cgggaagagg ccgcgcaggc gctgttcgca cttaatgatg
    35221 ttcgcactga gcgtctgagc gatcgcataa cgcaatggca attttttcac tgactccttc
    35281 aattttatct catggtcatt gtgagtcagc ggcgtaaaat taatgacatc agttaaggag
    35341 ttcgccatga aacatgacca ttttgttgtt caaagcccgg ataaacctgc gcaacagttg
    35401 ttgctgcttt ttcacggtgt cggcgataac ccggtagcaa tgggggaaat aggtagctgg
    35461 tttgcaccgc tgtttcctga tgcgctggtg gtgagtgtcg gtggcgcgga gccgagcggt
    35521 aatccggcgg ggcgtcagtg gttttcggtg cagggtatta cggaagataa tcgccaggcg
    35581 cgtgtggatg ccatcatgcc gacgtttatt gagacggtgc gctactggca gaaacagagc
    35641 ggagtagggg ctaacgccac ggcgctaatc ggtttctcac aaggcgcgat tatggcgctg
    35701 gagagcatta aagccgaacc ggaccttgcc tcacgcgtca ttgcctttaa cggacgctat
    35761 gccagcctgc cggaaaccgc gtctacagcc acgacaattc acttgattca tggtggtgaa
    35821 gatccggtta tcgatctggc gcatgcggtt gcggcgcagg aagcgttaat cagtgccggt
    35881 ggcgatgtga cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg
    35941 caatttgccc tggatcattt gcgttatacc attccgaaac attatttcga cgaagcgcta
    36001 agcggcggta agcctggcga tgatgatgtg attgaaatga tgtaataata gccgggtagg
    36061 cctgataaga ccgtatcagg cattatggcc ggatgcggcg tgaacgcctt atccggccac
    36121 tcagcttact tcttcggttg atcctttttc ggccagtcgt cgtcatcgtc ccacttatcg
    36181 ttgaaatcac gatgcggggg aagctccggc ttgttggcga gaaatttctt atggtcgacg
    36241 cgcttgagat ctttaatcac gttcagcagg acgcctacca aaaagacaat caccagaatc
    36301 caccaatatt tagccagcca gtccatgctt atttcctctt gcagaaccac tcatcaggcg
    36361 acgagctgtt ccatgatacg ttgatacata cgggcaagta gctgcaggtc ggcagcgttc
    36421 acacattcat taattttatg aatagtggca ttgaccggcc cgagttccac cacctgcgcc
    36481 cccatgcggg caataaagcg cccgtcggac gttccgcctg tggtcagtag ctgcggttta
    36541 atttcattat agtgctcaac cgcgttaacg accgcatcca ccagtttacc gcgcgcggtc
    36601 aaaaatggct gcccggaaag ccaccaatcc accgtatagc gcagttgatg tttttcaagc
    36661 agggcaagca cctgcgcttt gatcatctca tcagtcagtt cggtgctgaa gcggaagtta
    36721 aactgcacaa acagttcacc cggaataacg ttgttactgc ccgttcccgc ctgaatattg
    36781 gcaatctgca tactggtcgc cgggaagaat tcattgccct gatcccactc aatagccact
    36841 aattcattaa ggaaaggtgc tgcgcgatgt accggattgt cagccagatg tgggtaggca
    36901 acatgcccct gaacgccatg aatggttagg ttgcaggtta acgatccacg acgaccattt
    36961 ttcaccacat cacctaccac ttcgatactc gacggttcgc cgaccaggca gtaatcgaga
    37021 cgctcattac gtgccattaa cgcttcgacg acttttaccg taccgttgtg tgcactggct
    37081 tcttcatcag aggtgatcag aaatgctagt cgccccgtat ggttgggatg ttgtgcgaca
    37141 aaacgttctg ccgctaccac catcgccgcc agcgagcctt tcatatctgc cgcaccgcgc
    37201 ccgaataaca tgccgtcacg aatggttggt tcaaacggcg gattgatcca acggtcggcg
    37261 tcgccaggcg gcaccacgtc ggtatgcccg gcaaaggcta acgtttcacc ctgcccacgc
    37321 catgcccaaa aattctgcgt atcggcaaag tccatgcgtt caacggtaaa accgatcgcc
    37381 tgcaaacgtt caatcaacaa agcctggcat cctgcgtcat caggactcag ggaagggcgg
    37441 cgaataagct gttgtgtcag ctcaataacc gggcacgaca tagactacac ctcatggaaa
    37501 aattgctgat aactggaatc actgaaaccc agcagcatag gcttaccagg cgcgcagagc
    37561 aatggacgtt tgataattgc aggcatttca gtcattaatg ccgccgcaga ggccgcatcg
    37621 gtgattttat tgcgggtggt ttcgtccagt ttacgccagg ttgtaccacg ggtgttgagt
    37681 aacgcttccc agcctaattc gttgataaaa ccgttcaata attcgctgtc cagcccatca
    37741 acgcgataat catgaaaacg atagtcgatg ttattggctt ccagccaacg gcgagccttt
    37801 ttaatggtgt cacaattttt gatgccgtaa agtgtaacca taatgaatcc tttttcgatt
    37861 tatgagaata aatactcatt taagagtgaa aacattttgc ctgcaaatga ttatcaggaa
    37921 atataacat
//
LOCUS       DAZFFG010000033        35120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFFG010000033 DAZFFG010000000
VERSION     DAZFFG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.033"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1429
                     /gene="cdgI"
                     /locus_tag="ACHJ7J_002046"
     CDS             <1..1429
                     /gene="cdgI"
                     /locus_tag="ACHJ7J_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5000283.1"
                     /translation="LSLTKIDPGQNYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFI
                     FSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQI
                     LPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSL
                     ALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHT
                     ATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNY
                     TIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLL
                     SRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEV
                     FGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISL
                     VMTEADKALREAKSEGGNKVIIHHI"
     gene            1610..3100
                     /gene="dgcJ"
                     /locus_tag="ACHJ7J_002047"
     CDS             1610..3100
                     /gene="dgcJ"
                     /locus_tag="ACHJ7J_002047"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO5000284.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWHFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIASQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3143..3646
                     /gene="yeaK"
                     /locus_tag="ACHJ7J_002048"
     CDS             3143..3646
                     /gene="yeaK"
                     /locus_tag="ACHJ7J_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO5000285.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3647..3751)
                     /gene="yoaI"
                     /locus_tag="ACHJ7J_002049"
     CDS             complement(3647..3751)
                     /gene="yoaI"
                     /locus_tag="ACHJ7J_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO5000286.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3921..4367
                     /gene="yeaL"
                     /locus_tag="ACHJ7J_002050"
     CDS             3921..4367
                     /gene="yeaL"
                     /locus_tag="ACHJ7J_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO5000287.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4324..5145)
                     /gene="nimR"
                     /locus_tag="ACHJ7J_002051"
     CDS             complement(4324..5145)
                     /gene="nimR"
                     /locus_tag="ACHJ7J_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO5000288.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGRVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5242..6423
                     /gene="nimT"
                     /locus_tag="ACHJ7J_002052"
     CDS             5242..6423
                     /gene="nimT"
                     /locus_tag="ACHJ7J_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO5000289.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6478..6825
                     /gene="yeaO"
                     /locus_tag="ACHJ7J_002053"
     CDS             6478..6825
                     /gene="yeaO"
                     /locus_tag="ACHJ7J_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO5000290.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6847..7101)
                     /gene="yoaF"
                     /locus_tag="ACHJ7J_002054"
     CDS             complement(6847..7101)
                     /gene="yoaF"
                     /locus_tag="ACHJ7J_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO5000291.1"
                     /translation="MKIISFVLPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTTQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7284..8309
                     /gene="dgcP"
                     /locus_tag="ACHJ7J_002055"
     CDS             7284..8309
                     /gene="dgcP"
                     /locus_tag="ACHJ7J_002055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO5000292.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDMDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8342..8440
                     /gene="yoaK"
                     /locus_tag="ACHJ7J_002056"
     CDS             8342..8440
                     /gene="yoaK"
                     /locus_tag="ACHJ7J_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO5000293.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8443..8517
                     /gene="yoaJ"
                     /locus_tag="ACHJ7J_002057"
     CDS             8443..8517
                     /gene="yoaJ"
                     /locus_tag="ACHJ7J_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO5000294.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8576..8824)
                     /gene="yeaQ"
                     /locus_tag="ACHJ7J_002058"
     CDS             complement(8576..8824)
                     /gene="yeaQ"
                     /locus_tag="ACHJ7J_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5000295.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8972..9154)
                     /gene="yoaG"
                     /locus_tag="ACHJ7J_002059"
     CDS             complement(8972..9154)
                     /gene="yoaG"
                     /locus_tag="ACHJ7J_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO5000296.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(9158..9517)
                     /gene="yeaR"
                     /locus_tag="ACHJ7J_002060"
     CDS             complement(9158..9517)
                     /gene="yeaR"
                     /locus_tag="ACHJ7J_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO5000297.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9690..10328)
                     /gene="leuE"
                     /locus_tag="ACHJ7J_002061"
     CDS             complement(9690..10328)
                     /gene="leuE"
                     /locus_tag="ACHJ7J_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO5000298.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10455..11378)
                     /gene="dmlR"
                     /locus_tag="ACHJ7J_002062"
     CDS             complement(10455..11378)
                     /gene="dmlR"
                     /locus_tag="ACHJ7J_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO5000299.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11481..12566
                     /gene="dmlA"
                     /locus_tag="ACHJ7J_002063"
     CDS             11481..12566
                     /gene="dmlA"
                     /locus_tag="ACHJ7J_002063"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO5000300.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMIGSATTPQVADAICKIILR"
     gene            12817..14427
                     /gene="yeaV"
                     /locus_tag="ACHJ7J_002064"
     CDS             12817..14427
                     /gene="yeaV"
                     /locus_tag="ACHJ7J_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO5000301.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14459..15583
                     /gene="yeaW"
                     /locus_tag="ACHJ7J_002065"
     CDS             14459..15583
                     /gene="yeaW"
                     /locus_tag="ACHJ7J_002065"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO5000302.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15639..16604
                     /gene="yeaX"
                     /locus_tag="ACHJ7J_002066"
     CDS             15639..16604
                     /gene="yeaX"
                     /locus_tag="ACHJ7J_002066"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO5000303.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGVGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16658..17773)
                     /gene="rnd"
                     /locus_tag="ACHJ7J_002067"
     CDS             complement(16658..17773)
                     /gene="rnd"
                     /locus_tag="ACHJ7J_002067"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO5000304.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17855..19540)
                     /gene="fadD"
                     /locus_tag="ACHJ7J_002068"
     CDS             complement(17855..19540)
                     /gene="fadD"
                     /locus_tag="ACHJ7J_002068"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO5000305.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19745..20326)
                     /gene="yeaY"
                     /locus_tag="ACHJ7J_002069"
     CDS             complement(19745..20326)
                     /gene="yeaY"
                     /locus_tag="ACHJ7J_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO5000306.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(20366..21061)
                     /gene="tsaB"
                     /locus_tag="ACHJ7J_002070"
     CDS             complement(20366..21061)
                     /gene="tsaB"
                     /locus_tag="ACHJ7J_002070"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO5000307.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNSATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(21119..23029)
                     /gene="yoaA"
                     /locus_tag="ACHJ7J_002071"
     CDS             complement(21119..23029)
                     /gene="yoaA"
                     /locus_tag="ACHJ7J_002071"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO5000308.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            23161..23505
                     /gene="yoaB"
                     /locus_tag="ACHJ7J_002072"
     CDS             23161..23505
                     /gene="yoaB"
                     /locus_tag="ACHJ7J_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5000309.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            23927..24226
                     /gene="yoaC"
                     /locus_tag="ACHJ7J_002073"
     CDS             23927..24226
                     /gene="yoaC"
                     /locus_tag="ACHJ7J_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO5000310.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(24346..24525)
                     /gene="yoaH"
                     /locus_tag="ACHJ7J_002074"
     CDS             complement(24346..24525)
                     /gene="yoaH"
                     /locus_tag="ACHJ7J_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO5000311.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            24599..25960
                     /gene="pabB"
                     /locus_tag="ACHJ7J_002075"
     CDS             24599..25960
                     /gene="pabB"
                     /locus_tag="ACHJ7J_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO5000312.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            25964..26542
                     /gene="nudL"
                     /locus_tag="ACHJ7J_002076"
     CDS             25964..26542
                     /gene="nudL"
                     /locus_tag="ACHJ7J_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO5000313.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            26726..28090
                     /gene="sdaA"
                     /locus_tag="ACHJ7J_002077"
     CDS             26726..28090
                     /gene="sdaA"
                     /locus_tag="ACHJ7J_002077"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5000314.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            28221..29819
                     /gene="pdeD"
                     /locus_tag="ACHJ7J_002078"
     CDS             28221..29819
                     /gene="pdeD"
                     /locus_tag="ACHJ7J_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5000315.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYLVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(29823..31379)
                     /gene="yoaE"
                     /locus_tag="ACHJ7J_002079"
     CDS             complement(29823..31379)
                     /gene="yoaE"
                     /locus_tag="ACHJ7J_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO5000316.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(31367..31525)
                     /gene="yoaL"
                     /locus_tag="ACHJ7J_002080"
     CDS             complement(31367..31525)
                     /gene="yoaL"
                     /locus_tag="ACHJ7J_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO5000317.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            31842..32813
                     /gene="manX"
                     /locus_tag="ACHJ7J_002081"
     CDS             31842..32813
                     /gene="manX"
                     /locus_tag="ACHJ7J_002081"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO5000318.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            32876..33676
                     /gene="manY"
                     /locus_tag="ACHJ7J_002082"
     CDS             32876..33676
                     /gene="manY"
                     /locus_tag="ACHJ7J_002082"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO5000319.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            33689..34540
                     /gene="manZ"
                     /locus_tag="ACHJ7J_002083"
     CDS             33689..34540
                     /gene="manZ"
                     /locus_tag="ACHJ7J_002083"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO5000320.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            34595..35053
                     /gene="yobD"
                     /locus_tag="ACHJ7J_002084"
     CDS             34595..35053
                     /gene="yobD"
                     /locus_tag="ACHJ7J_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO5000321.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
BASE COUNT         8642 a         8888 c         8576 g         9014 t
ORIGIN      
        1 tttatcgtta acgaaaattg atcctggtca aaactatata tctctgccat caataaaatc
       61 cagcactcac attgctctcc tttttatggt ttctatgggt acacaaaaat taaaagctca
      121 aagctttttt attttcagtt tattgctgac gttaatttta ttttgcatta ctaccttata
      181 taacgaaaac acaaatgtaa aactcatccc acagatgaat tacctgatgg ttgttgtggc
      241 tttgtttttc cttaacgccg tcatttttct tttcatgtta atgaaatatt tcactaacaa
      301 acaaatttta ccaacactca ttttaagcct tgcattttta agtggcctta tctatttagt
      361 tgaaaccatt gtaattatcc ataaaccaat taacggcagt acactgatcc agacaaagtc
      421 gaatgatgtt tctattttct atattttccg ccaactcagt tttatttgtt taacctcgct
      481 ggcgctcttt tgttatggaa aagacaacat ccttgacaac aataagaaaa aaactggcat
      541 tctgttgctg gcgctgattc cttttttagt ttttcccctt ctggcacaca atctgagcag
      601 ttataacgct gactattctt tgtatgtcgt cgattactgt ccggacaacc atactgcgac
      661 ctggggaatc aactatacaa aaatattggt ttgtctgtgg gcatttttac tgttctttat
      721 tatcatgcgt acacgattag ccagcgaact atggccgtta atagcattat tatgtctggc
      781 atcgctatgc tgcaacttac ttctactgac tctggatgag tataattata ccatctggta
      841 tatcagtcgc gggattgaag tctccagtaa actgtttgtt gtgtcttttc tgatttataa
      901 catttttcag gagctgcaac tctccagcaa actggcagtt catgatgtgc tgaccaatat
      961 ttacaatcgg cgctactttt tcaacagcgt agagtcatta ttgtcgcgac ctgttgttaa
     1021 ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa cgcatcaatg cccaatgggg
     1081 acatcgtgtg ggtgataaag tgctggtttc aattgtcgat attatccagc aaagcatccg
     1141 ccccgatgat attttagcgc gactggaggg tgaggtgttt ggcttgctat ttaccgaact
     1201 caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt aaaaatgtcg aactcctgac
     1261 cggctttagt aacagatatg atgttcctga acaaatgacc atcagtattg gcacggtttt
     1321 ttcaacgggt gacacgcgta atatctcgct tgtcatgacg gaagcagata aagccttacg
     1381 cgaagcgaaa agcgaggggg gcaacaaagt gattattcat catatttaag tgcaaaaata
     1441 ttcagagcca tgctttttgc gtggcttttg catacaattt attactatta cccctaaatt
     1501 tcccctcccc agcggcgcag agatgagtat aattagcgcc cctgtgccag gccgcaatcg
     1561 aactttatct ggttttctcg tttcactaac cgaaggagtg ccatttatca tgaaattgca
     1621 ccatagaatg ctccggcatt ttatcgccgc aagtgtcatt gtgctgacat cttccttcct
     1681 tatttttgaa cttgtcgcca gcgacagagc aatgagtgcc tatctgcgct atatcgtgca
     1741 gaaagcagac tcctcctttc tttatgataa gtatcagaat cagagtattg ccgcgcatgt
     1801 gatgcgcgct ctcgctgctg agcagtcgga agtgtcgcca gaacagcggc gcgccatctg
     1861 cgaggctttt gagtctgcca ataataccca tggcttaaac ctgactgccc ataaataccc
     1921 gggcttacgc ggcacactac aaaccgcatc cactgactgc gacacaattg tggaagctgc
     1981 agcactatta cccgcttttg atcaggcagt ggaaggcaac cgccaccagg atgattacgg
     2041 ttcaggtctt gggatggccg aagagaaatt tcactattat ctcgatctca atgaccgcta
     2101 tgtctatttt tatgagccgg ttaatgttga atactttgcg atgaataact ggtccttcct
     2161 gcagtcagga agtattggca tcgatcgcaa agatattgaa aaggtattta ccgggcgtac
     2221 cgtattgtcg agcatttacc aggatcagcg tactaaacag aacgtgatga gtttgctgac
     2281 gccggtgtat gttgcagggc agctaaaagg gattgtgctg ctggatatta acaaaaacaa
     2341 tctgcggaat atcttttaca ctcatgaccg ccctctcctc tggcattttc tcaatgtcac
     2401 gctaaccgat accgattcgg ggcgcgacat tatcatcaac cagagcgaag ataatctgtt
     2461 ccagtatgtc agttacgtcc atgacttacc gggcggcatt cgtgtctcgt tatccattga
     2521 tattctttac tttatcacgt cttcgtggaa aagcgttctg ttctggattt tgacggcgtt
     2581 aattttgctg aatatggtgc ggatgcactt ccgcttatac caaaatgtgt cgcgagaaaa
     2641 tattagtgat gcgatgactg gactgtataa tcgcaaaatt ttaacccctg aactggagca
     2701 gcggttgcag aaactggtgc aatccggttc ttcggtgatg tttattgcaa ttgacatgga
     2761 caagttaaag ctaataaatg acaccctcgg tcatcaggaa ggggatttag cgattacgtt
     2821 gttagctcag gcgattaaac aatcgattcg taaaagtgat tatgccatcc gactcggtgg
     2881 cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa attgcatcac aactgcctga
     2941 acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa gagatcggct tctcttccgg
     3001 tatttacgcg atgaaagaaa acgatacgtt acatgatgcg tataaagctt ccgatgagcg
     3061 tttatatgtc aataagcaga acaaaaacag ccgttcatga taaccttctg tggttgtttg
     3121 cttgtaatct caggagcatg aaatgactga aatggctaaa ggaagcgtga cgcatcagcg
     3181 gttaatcgct ttattatcac aagaaggtgc agacttccgc gtcgttaccc atgaagcggt
     3241 aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct ttaggccagg gggctaaggc
     3301 actggtatgt aaagtcaaag gcaatggcgt aaatcaacat gttctggcga tcctcgctgc
     3361 cgatcaacag gccgatctga gccaactcgc cagccatata ggcggattac gcgcttcgtt
     3421 ggccagcccg gcggaagtcg atgaactcac aggctgtgtc ttcggcgcga tccccccttt
     3481 cagcttccat ccaaaactca aactggttgc cgacccccta ctcttcgaac gatttgatga
     3541 aatcgccttc aatgcaggca tgctggataa atccgttatt ttgaaaaccg cggattattt
     3601 gcgcattgcg caaccagaac tggtcaattt ccgccgcact gcgtaactag cctgtccgtt
     3661 caataagcag aacggacaaa accagcacaa cagcaatggc aaaaaacgat gacgtgataa
     3721 tcagtgtctc gacgaacatt tgatcgttca tagcatgccc ccgggagata gctgttttca
     3781 cgttattgtt agcgtgcaca aatggcagtt tgatgacagt tcgcctgatt ttttatgcaa
     3841 aaaacgtaaa gattttctac ttccttcctg cagcaagcgt aaagtaagca ggcttattat
     3901 tttttggcaa ggaaaccacg atgtttgatg tcactttgct gatcctgctc gggttagctg
     3961 cgctgggctt tatcagtcat aacaccactg tcgccgtttc aattctggtg ttgatcattg
     4021 tccgcgtcac accgttaagc acctttttcc cgtggattga aaaacagggg ctaagtatcg
     4081 ggataatcat tctgacgatt ggcgttatgg cccccatcgc cagcgggacg ctaccgccct
     4141 caaccttgat tcactcgttt ctgaactgga aatcgctggt ggcaattgca gtaggcgtta
     4201 ttgtctcctg gctgggtggg cgcggcgtga cgttaatggg cagccagccg caactggttg
     4261 ctgggctgct ggtgggcact gttttaggcg tagcgctgtt tcgcggcgta ccggttggcc
     4321 cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa acagtagtta atcccgcaat
     4381 atatcggcct ggcgtttgcc ccaggccttt cttaaacata gtaataaagg ccgttgttga
     4441 atcatacccc agcgtatgag caactttctg tacagtatcg cctttcacta atccctgaag
     4501 cgccataatc agctgtaact gctgacgcca ctggcgaaaa ctcaaccccg tctcttttac
     4561 tatcagtcgg gcaagattgc gttcactcat cgcaaaaaag ccagcccatt gccccaatga
     4621 cccccactcg acgggccctt tcgccatcat ctccaccatc gtgcggattt taggatgaga
     4681 agaaacgggc aggtgcagct gttgttgtgg ctgctgaggg agttcatcaa acaggacctg
     4741 aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc gtcctgtttg ccagggttaa
     4801 aatcaattcc cggcataagg gcgatatttt caatgtgcaa catgttgtcg gcattgtcac
     4861 tgcgctgggt tcgataaaca gaaaacagag ttcagcgtta gctgtcactt gattgctatg
     4921 ttccaccctg cccggtatcc aaaccgcata ttgcggcggc accatccaca aagcattttc
     4981 caccgtacag gtaattgcac catgtaatgc tagtatcagc tgtcctttgc ggtgctgatg
     5041 tacaggactg gttaactcat ccgttccggc gtgaatacaa aatgcgaccg cggcttcatg
     5101 gtgtcggtcg ggttcatagc cattgagatt taacctgtgc atcattttgt ccgaatttag
     5161 cgataatttg tcattttagc ttgattcaac ataacaataa aaacggtaag gtacagcctc
     5221 gtttgtaaca atgagaagca tatgacctgt tcaacttcat taagcggcaa aaacaggatt
     5281 gtccttatcg ctggcattct gatgattgcc actacattac gcgtcacctt caccggcgca
     5341 gcaccgttac tcgatacgat tcgttccgct tactcgctga cgactgcgca aaccggctta
     5401 ttgaccaccc tgccattatt ggcctttgcg ctaatctcac ctctggctgc cccggtggct
     5461 cgacgttttg gtatggaacg tagcctgttt gccgcgttac ttttgatctg tgcaggtatc
     5521 gcaattcgct ctctcccttc gccttactta ttatttggcg gtacagcggt cattggcggt
     5581 gggattgcat taggcaatgt cttactgcca ggattaatta aacgcgattt ccctcattcc
     5641 gtcgccagac ttaccggcgc atattccctg acaatgggag ctgcggcggc actgggatcg
     5701 gctatggtag tgccactggc tttgaacggt tttggctggc aaggcgcgtt gctcatgctg
     5761 atgtgttttc ctctgctggc tcttttttta tggctgccac agtggcgaag tcaacaacat
     5821 gcaaatttga gtacctcgcg cgccttacat actcggggta tctggcgttc gccgcttgcc
     5881 tggcaggtaa cattgtttct tgggatcaac tcactgatct attacgtaat tattggctgg
     5941 cttccggcga tcctcatcag tcacggctat agcgaagcac aggcgggttc actgcatggt
     6001 ttgctgcaac tagccacagc agctcccggt ttgctgatcc cacttttctt acatcatgtg
     6061 aaagatcagc gtggtattgc agcgttcgtt gccttgatgt gcgcagtggg cgcggttggg
     6121 ctctgcttta tgccagcaca cgcgatcacc tggactctgc ttttcggttt tggttccggc
     6181 gcaacaatga tactggggtt gacgttcatt ggtcttcggg caagttctgc gcatcaggcg
     6241 gcggcactct cggggatggc acaatccgtc gggtatttgc tggcagcctg tgggccgccg
     6301 ctgatgggta aaatacacga tgctaacggt gactggtccg taccactaat gggtgttgca
     6361 atactgtcac tactgatggc gattttcgga ctttgcgccg ggagagacaa agaaattcgc
     6421 taatatccgg tgctatagtg acgtgacaaa tcatgcgtga aagggagaac aaacacgatg
     6481 aatattcagt gcaaacgcgt ttatgatccg gcagaacaga gcgatggtta tcgcgtactg
     6541 gtcgaccgcc tctggccgcg tggtatcaaa aaaaccgatt tagcccttga tgagtgggat
     6601 aaagaaatca cgccgtcaac ggaactgcgc aaagcctttc acggcgaagt cgtcgatttt
     6661 gcaacctttc gcgagcaata tcttgcagaa ctggcgcaac acgagcaaga aggaaagcgg
     6721 ctggcggaca tcgccaaaaa acagccgctg accctgcttt actcagcaaa aaacaccacg
     6781 cagaaccatg cgctggtgct ggccgactgg ctacgtagct tgtgatttta gtacagcatc
     6841 cggcggttat ttttcaccag ccggatggtc ccgtcgccac aatgcccatt catcaatcgt
     6901 ttcaccgccc ggtaatttgc aattgttgct gaccccttgc gttgtctgca ctggaatgag
     6961 cgtcccgccc ttctgctggc aatagaccga cgccggattt gccataccaa tctgcggcgg
     7021 tttaggtgct tctggctgag aagaggttga acaaccagcc agaaccagca agcaaggcag
     7081 aacaaaactg ataattttca tttattgatc tcacatattt atccaagctt agagtatcgc
     7141 ggtatcgttt tgttttgcag cactattttt attacattca ctcaaatcat attacgcctt
     7201 gtttcatctt tgttgatgat gttttatcat gcctgcaaag attaaataat cagcatttac
     7261 ccgccgtatc ctggagttgt ttcgtgtcag atcagattat cgcccgcgtc tcgcaatccc
     7321 ttgccaaaga acagtcactg gaaagcctgg tccgacagct tctggagatg ctggaaatgg
     7381 tcactgatat ggaatcaacc tacctgacca aagtggatgt cgaagcgcgc ctgcagcata
     7441 taatgtttgc ccgtaacagc cagaaaatgc acatcccgga gaattttacc gtctcgtggg
     7501 attactcgtt atgcaaacgc gccattgatg aaaactgctt tttcagcgat gaagtccccg
     7561 accgttgggg cgactgtatt gcggcacgca atcttggcat caccacattt ctgagcacgc
     7621 caattcactt accggatgga tcattctatg gcacgctttg cgccgccagc agtgagaagc
     7681 gccagtggag tgaacgcgcg gaacaggttt tgcagttatt cgccggactg attgcacaat
     7741 atattcaaaa agaggcgctg gtagaacagc tgcgcgaagc caatgccgcg ctgatagcgc
     7801 aatcgtatac cgactcgtta accgggttac caaatcggcg ggcgattttt gaaaatctga
     7861 cgacgctgtt ttccctcgcc cggcatctta accataagat aatgatcgcg tttatcgata
     7921 tggataactt caaattaatc aatgatcgtt ttggtcataa tagtggcgat ctgtttctca
     7981 ttcaggttgg cgagcgcctt aatacgctcc agcaaaatgg cgaagttatt ggtcgtctcg
     8041 gtggtgatga gtttttggtt gtttcactaa acaacgagaa tgcggatatt tcgtcgctgc
     8101 gagaacgtat tcaacagcaa atacgtggag aatatcactt aggtgatgtt gatttgtatt
     8161 atcccggtgc cagtcttggc atagtagaag tcgatcctga aacgaccgat gcagacagtg
     8221 ccctgcatgc tgccgatatc gcgatgtatc aggagaaaaa acacaaacag aaaacacctt
     8281 ttgtcgcgca tccagcgcta cattcctgag gcgtattcac atccttttga ttggtgataa
     8341 catgcgaatc ggtattattt ttccggttgt aatcttcatt acagcggtcg tatttttagc
     8401 atggtttttt attggcggct atgctgcccc gggagcataa agatgaaaaa aacaacgatc
     8461 attatgatgg gtgtggcgat tattgtcgta ctcggcactg agttgggatg gtggtaacgt
     8521 cgcatctaaa aaatagcaaa ggctgcctgt gtgcagcctt tgtgcaattt acgcgttaac
     8581 ttttaatctt cctgtagata aatagcacga caatcgcacc aataacggca accacgaagc
     8641 tgccaaaatt gaagccatcg actttaccaa agccaaacag cgtgctgatc caaccgccga
     8701 ctacggcacc gactatcccc agcaggatag tcataaagaa tccaccacca tctttacctg
     8761 gcatgatcca cttcgccaga ataccggcaa taagcccaaa aataatccat gacagaatgc
     8821 ccattgtttc ctcacttatc tgttttgcat tagcgggtta gtcgctgata aaaagcatag
     8881 cacaacatcg ggagggcaag atttgtgacg agcatcacgg aggatttttt tgcgatggcg
     8941 cagaaattgc gccatcaacg atcagtgata attaccaacc acaaacatca tgttcgtttt
     9001 ccgtgtcata agaacgcacg gtattcacca gatcttttat cacttcagcc gccacttctg
     9061 gcaccagcaa agtcatcggc gtctctgttt cataatcgac agaaacgcca ttgctgttat
     9121 tggtgacggt cacggtatac gttgctttgc ccatgattca tttcccgtta tgaatgactt
     9181 tccgttgttg cgcaccttcc atcaggactt caggagccac gaagaagtca atgttgaaat
     9241 aagtatcgtc agtcatggct tcaatgttgt gccacttttc tggagggaac accgcaaact
     9301 gccccgcttc gataaggatc acctgatcag gctctgcact gtgttcatca gcgtacccga
     9361 gatatttgac cgccccatgc ataacggaaa ggcgtgggta aacccccggg cgcgttcctt
     9421 tatcaagatg acgttcgaat attccggcag gcgcagtttg tttattccag aaaggcgttg
     9481 agcgcgtatg aatataattc tgtgggattt gaagcatcct tttccctcct tcggtgaatg
     9541 cgctgaaaac ggtttattcc agccgtttca gggtacgcct gataatttgc atttaaaata
     9601 ccatttattg gttatttttt agcaccatat cagagaagaa tcagggagga ttatagatgg
     9661 gaagcccgcg acaacgcggg ctgaaagcat caggattgca gcgtcgccag tcgggcagcg
     9721 aaacccacga acatcaaacc aatcagtgag ttgcccactt tagccagttt ctttttggta
     9781 cgtatgtact gcgtgacaaa agcaccagaa ataatcagga agctcaaata gcagaaactc
     9841 accagttcca gcgtcgtcgc cagaataaag aatgaaattc ccgtatgtgg ggcattaaca
     9901 tcgataaact gtacgaaaaa cgacacatag aacaaaatgg ctttcggatt agtcaggctc
     9961 aaaattaacg cgcgtttaaa aatggcaccg tattggggct catcggattt ggcctcgtta
    10021 tttttaccct tcagggtcgc gtagagaatt ttactcccca gatagagcaa ataaaatgca
    10081 ccaagataac gtacaatgtt gaataatatc ggggtggtct taattaatgt cgccactcca
    10141 gcccatgcca gaaacatcaa taccgcatcg ccaataaata caccgcaggc cgcaagataa
    10201 ccgcctttca taccgctact gacgctattt ttgagtacaa acagggtatt tggccctggc
    10261 accaacacaa taaaaatggc cccaaccaga taggtccagt aattcagaac cccgtattca
    10321 gcgaacacat taacctcttt aattatcttt cgatcatgcg cgattaaagg tgaatatgct
    10381 aaccaatctg tagcagctta gaaaggagaa aatcaggttt taacctgata tcaacccgat
    10441 aattgaatca ttaatcaggc atgctccagt gaaaaattcg ggtagtgctc ggcaaaatac
    10501 tggcgtaaaa attctaccgt tatccgcact ttcgctgacg tcgccagcct tgaaacataa
    10561 acggcccaga cgttcgctgg ctggtaatat tccggtagca cttgcactaa atgaccactg
    10621 gcaatgtttt cgctaacatc ccaccaggaa cgcagagcaa tcccctgccc atcgagacac
    10681 cactgatgga caatttctcc atgattagac gacagtgggc cggtgacttt aatcgcatgg
    10741 tggccttctt tattgcgtaa ttgccagaca ccaaaaggat gatcgcgctc tttaatgacc
    10801 aggcacggca atgcagaaag atcggttaaa tgtttcggcg cgccatgttg cgcaataaat
    10861 tccggcgacg cgcagagaat gcggtaattg gtcgccagtt tgcgggcgat taaattaggg
    10921 gcgatgtcat cgccaatgcg aatatcgaga tcgacacctt cattgaccaa atcgaccagc
    10981 cggtcttcca catcaaaacg taattccagt tgcggatacg ccttcgccag cgctgataac
    11041 gccggagcca ccacctgtcg tccaaaacca aagctgctga taatacgcag catcccctgt
    11101 ggcacctgac gcacgtcaga aagttcgtcc atcatctgac cgacatcctg caaaatccgc
    11161 tgcgcccatt cataaatccg ctctccttct tcggtaatgg tgacgcggcg ggtggtgcgg
    11221 tgtaacaaca ccacgtttag cgtttgctcc agcaaggcga tgcgcttgct gacgaacgcc
    11281 ggtgaaacgc ccagttcttc cgccacggcg gcaaaaccag cccggcgagc caccagcata
    11341 aagacgcgca aatcattcag cagcggtaaa ttattcatga ttcgtgtttt atgtttcacc
    11401 agttacgggg attaattcct tttcagtcaa ttataggatg gtgatgttgt caattttgat
    11461 ggtcaggaag tgagaaccca atgatgaaaa cgatgcgtat tgctgcgatc ccgggagacg
    11521 ggattggcaa agaagtcctt cctgaaggga ttcgcgtgtt acaggctgcc gctgagcgct
    11581 ggggcttcgc cttgagtttt gagcaaatgg agtgggcgag ctgcgagtat tacagccatc
    11641 acggtaaaat gatgccggac gactggcatg agcaacttag ccgtttcgac gccatctatt
    11701 ttggtgccgt cggctggccg gacaccgttc cggaccatat ttcgttgtgg ggttcgctgc
    11761 tgaaatttcg tcgtgaattc gaccagtacg tcaacctgcg cccggttcgt ctctttcctg
    11821 gcgttccctg cccgctggcg ggaaaacagc ctggcgacat cgatttttac gtggtcaggg
    11881 aaaacaccga aggcgaatat tcctcgctcg gcggtagagt gaatgaaggt acagagcatg
    11941 aagtcgtcat tcaggaatcg gtatttacgc gtcgtggtgt cgatcgcatt ttgcgttatg
    12001 ccttcgaact tgcgcaaagc cgcccacgta agacgctaac ttctgccact aaatcaaacg
    12061 gtttagccat cagcatgccg tactgggatg agcgagtgga agcaatggcc gagaattacc
    12121 cggagatccg ctgggacaag cagcatattg atattctctg cgcgcgtttt gtgatgcagc
    12181 cggaacgctt cgatgtggtg gtggcgtcca atttgtttgg cgatatcctt tccgatcttg
    12241 gcccggcctg caccggcacc attggcattg ccccatccgc caacctgaat ccggaacgca
    12301 ctttcccgtc gctcttcgag cctgtccacg gttccgcgcc ggatatctac gggaaaaata
    12361 ttgctaaccc tatcgccaca atttgggccg gtgcaatgat gctcgatttt ctcggtaatg
    12421 gcgatgagcg tttccagcaa gcgcataacg gtattctggc agcgattgaa gaagtgattg
    12481 ctcacgggcc gaaaacaccg gatatgatag gcagtgccac cacgccacag gttgccgacg
    12541 cgatttgcaa aattattttg cgttaaggtc aaaccagttt atttgaaccg cgtcactgac
    12601 gcggtttttt tattcgttct ttgcagttaa taacctgcgt catttcacct tttattgttt
    12661 ccgtttcgtg ttttatggct ttccgtattc ttaattgttt aatttatgta acatgcaaat
    12721 aatcaatttg ttaaacacaa catacaactc attaccctgc aaataattac aactggcaaa
    12781 agccagagac gggcttttat gcccaggaga tgactcatga tgagcaatgt taagaaaaaa
    12841 gatgtgccgc tgataagcat cagcctggtg gccattcttt tcatcgcagc tgcattaagc
    12901 cttttcccac aacaatcggc cgacgcggcc aacgcaatat acacttttgt tacgcgtacg
    12961 ttaggttccg ccgtacaggt attggttttg ctggcaatgg gactggtgat ttatttagcc
    13021 accagtaaat acggcaatat tcgtcttggc gaaggaaaac cggaatacag cacgctctcc
    13081 tggctgttta tgtttatttg tgccggttta ggttcttcta cgctttattg gggggttgct
    13141 gaatgggcct attattatca aacgcctgga ttaaatatcg caccgcgttc acaacaggca
    13201 ctcgaattta gcgtccctta ctccttcttc cactggggca tcagcgcctg ggcaacttat
    13261 acgctggcct cattaatcat ggcttatcac tttcatgtgc ggaaaaacaa aggtctgagt
    13321 ctttccggca ttattgccgc gattaccggc gttcgcccgc aaggcccatg gggaaaactg
    13381 gttgatttga tgttcctgat cgccactgtc ggcgcactga ctatttccct tgttgtcacc
    13441 gcagcaacct ttactcgtgg actttccgca ctgaccggtt tacccgataa cttcaccgtg
    13501 caggcatttg tgatcctgct ttccggcggc attttttgcc taagctcatg gattggtatc
    13561 aacaacggtt tgcaacgtct gagcaaaatg gttggctggg gcgcgttcct gctgccatta
    13621 ctggtgctga ttgtcggtcc aaccgaattt attaccaaca gcatcatcaa tgccatcggc
    13681 ctgaccacgc aaaacttcct gcaaatgagc ttattcaccg atccgcttgg cgatggttca
    13741 tttacccgca actggaccgt tttctactgg ctgtggtgga tctcatacac ccctggcgta
    13801 gcaatgtttg tcacccgcgt ttcccgcggt cgtaagatta aagaagttat ctggggactg
    13861 atcctcggca gcaccgtcgg ttgctggttc ttcttcggcg taatggaaag ctatgccatt
    13921 catcagttta tcaatggcgt aatcaacgtc ccacaggtgc tggaaactct gggcggcgaa
    13981 acagctgtac agcaagttct gatgtcgttg ccagctggta aattgttcct cgccgcatac
    14041 ctgggcgtga tgattatttt ccttgcctcg catatggatg cagtggccta caccatggcg
    14101 gcgaccagta cgcgtaatct ccaggaaggt gacgatcctg accgtgggct gcgtcttttc
    14161 tggtgcgtgg tgatcactct gatcccgctt tccatcttgt ttaccggtgc ttcgctggaa
    14221 acgatgaaaa ccaccgtcgt gctcacagcc cttcccttcc tcgtcatttt actggtgaaa
    14281 gtcggcggat ttattcgctg gctgaaacag gattacgccg acattccggc tcatcaagtt
    14341 gaacattatc tcccgcagac accggttgaa gccctggaaa aaacaccagt gctccctgcg
    14401 ggaaccgtat tcaaaggcga caactgagcg ccatcatcct aacgataaag gtatccctat
    14461 gagcaatctg agccctgact ttgtactacc cgaaaatttt tgcgctaacc cgcaagaggc
    14521 gtggaccatt cctgcccgtt tttataccga tcagaacgcg tttgaacacg aaaaagagaa
    14581 cgtcttcgcc aaaagctgga tttgcgtcgc tcacagcagc gaactggcga atgccaatga
    14641 ttatgtgacg cgggagatca ttggcgaaag catcgtgctg gtacgcggtc gtgataaggt
    14701 tttgcgcgcg ttctataacg tgtgtccgca ccgtggtcat cagttgttga gcggtgaagg
    14761 aaaagcaaaa aatgtgatta cctgcccata tcacgcatgg gcattcaaac tcgatggcaa
    14821 tctggcccat gcacgtaact gcgaaaacgt cgccaatttc gatagcgaca aagcgcaact
    14881 ggttccggtg cgtctggaag aatatgccgg attcgtcttc atcaacatgg accccaacgc
    14941 caccagcgta gaagatcagt tacccggtct gggcgcgaaa gtgctggaag cctgcccgga
    15001 agtccacgat ctgaaactgg cggcccgctt taccacccgc acgcctgcca actggaagaa
    15061 cattgtcgat aactatctcg agtgctatca ctgtggtccg gcgcatccag gtttctccga
    15121 ctccgtacag gttgatcgtt actggcacac catgcacggt aactggacgc tgcaatacgg
    15181 tttcgccaaa ccgtccgaac agtcgtttaa atttgaagag ggtacggatg cggcattcca
    15241 cggtttctgg ctgtggccgt gcacgatgct gaacgtcacc ccgatcaaag ggatgatgac
    15301 ggtcatttat gaattcccgg tggattctga aactaccctg caaaactacg atatttactt
    15361 caccaatgaa gagttaaccg acgagcaaaa atcgctgatt gagtggtatc gcgatgtgtt
    15421 ccgtccggaa gatttacgtc tggttgaaag cgtacagaaa gggctgaaat cgcgtggcta
    15481 tcgtggtcag gggcgcatca tggccgacag tagcggtagc ggcatttccg aacatggtat
    15541 cgcccatttc cataatctgc tggcgcaggt atttaaggac taatgacatc ggcggcggta
    15601 ttttccgccg ccgggctgat ttttgatgga gtacagcaat gtcagactat caaatgtttg
    15661 aagtacaggt gagtcaggtg gaaccgctta ccgaacaggt gaaacgcttc acgctggtgg
    15721 caaccgatgg caaaccgctg cctgcgttca ccggaggaag tcacatcata gtgcagatga
    15781 gcgatggtga taaccagtac agcaatgcgt attcactact gagttcaccg cataacacct
    15841 ctagttatca gattgccgtt cgactggaag aaaactcgcg cggcggttcc cgctttttgc
    15901 atcagcaggt aaaagtgggc gatcggttaa cgatctcaac gcctaataac ctgtttgcgc
    15961 taattccctc agccagaaag catctgttta tcgcgggcgg tgttggtatc acccctttcc
    16021 tgtcgcacat ggcagagttg caacacagcg acgtcgactg gcagctacat tactgctcgc
    16081 gaaatccaga aagttgcgca tttcgtgatg agctagtcca gcatccgcag gctgagaaag
    16141 tccatttgca tcattcatca accggaacac gactggaatt agcgcgatta ttggcggata
    16201 tcgaacctgg cacacacgtt tatacctgtg gccccgaggc gctaattgaa gcggtaagaa
    16261 gtgaagctgc gcgtctggac atcgccgccg atacgctgca ctttgagcaa tttgctatcg
    16321 aagacaaaac cggcgatgca tttaccctgg tgcttgcccg ttccggaaaa gagtttgtgg
    16381 tgccggaaga gatgactatt ttgcaggtta ttgaaaataa taaagccgcg aaagtggaat
    16441 gtttatgtcg tgaaggggta tgcggaacct gcgaaacggc aatactggaa ggtgaagctg
    16501 accatcggga tcagtatttt agcgatgaag agcgtgccag ccagcaaagt atgttgatct
    16561 gttgttcgcg tgcgaagggt aaacgcctgg tgttggattt gtagtttgcc gaagccggat
    16621 gtggcgctga gcgcgcccag tccggcttcg gaagatttta ctgcggatat tcctgcaata
    16681 aattgtgtaa tgcttccgcc atcagctcac cacgccagcc ggaaatcagc tccggcaaat
    16741 tgttctgcgg tttcagtttc cagtgccagt tcagcagttg gttgatttgc cgacgcgatg
    16801 ccagcaattc ggcgctgatc ttgtgcgttt cgctcacgtc agtaatcagc gacttaatcg
    16861 ctttaaacgc tttacgataa cccggcatgt ccatcaggtt aagcatcggc tgcggtaagg
    16921 cctcttccgg caatgtctgc gctttttcca ccagcgccag cagcgtttta ccgtgaaagc
    16981 ggatttcgct accggataaa cccaggctgt ccagttcgcc taaacttccc ggcatataac
    17041 gcgctaccga ccacaaatgc tcttcacgca cgacaaagtt caccgccaga tcgcgctctc
    17101 gcgccttgcg cagtcgccag tcggctaaca gttgcagaca ggccagttgg cgtgtgcgta
    17161 attgccaggc attggtgata tcacgccagg catcttccgg cgcaacgact tcctgacgac
    17221 gcatttgcat caggcggcat tcatccagcg ccgctggtag ccagccggag gcctccgttt
    17281 ctaccataag cttggcggtg atcggtaaca gataccagac atccgccgct gcatattcac
    17341 actgacgttc ggtcagcggt ctggccagcc agtcggtgcg cgattcactc ttgtccagcg
    17401 taacgccgga atactcttcc accatggaag caaaacccca tgacatcggg cgtccgcaga
    17461 aggcagcaag gatttgcgtg tcaatcaggg gttgtggtaa ttcgccaaag acattgagga
    17521 acacttccag atcttcactg cctgcatgga gaaattttgt gatggacgga tcgcgcagga
    17581 tcgctttcag cggtgaccag tcggtgatcc cgagaggatc aattagcgcc agatgctcgc
    17641 catcgaaaag ttgaatcaac cccagctgcg ggtaataagt gcgcgtacga acaaattcag
    17701 tatccagggc tatcgccgga aaggcacgga cggcttcaca caaagaagcc agcgcatcgt
    17761 ccgtggtaat catttggtaa ttcaaattgt attctcttta gtgggcgtca aaaaaaacgc
    17821 cggattaacc ggcgtctgac gactgactta acgctcaggc tttattgtcc actttgccgc
    17881 gcgcttcgtc acgtaattct cgtcgcaaaa tttttccgac gttagatttc ggtaactcat
    17941 cacgaaactc caccagcttc ggtactttgt agcccgtgag ctgacggcgg caaaaagtca
    18001 ccagtgactc ttcggtaagc gatggatctt ttttcactac aaagattttt accgcttcac
    18061 cactggagcc ggaaggtacg ccaacagccg cgacttcctg tacgccagga tgctgcatga
    18121 cgacatcttc aatctcgttg ggatagacgt taaaaccgga aaccagaatc atgtcttttt
    18181 tacgatcgac aatgcgcagg aacccttctt catccatcac cgcgatgtcg ccggtgtgta
    18241 accagccatt tttgatgatc tcatctgtag catccggacg ctgccagtaa cccagcatca
    18301 cctgcggtcc tttgacacaa agctcacccg gttgacccgg tggtacttca ttatcatcat
    18361 catccaccag tttggcttcc gtcgacggca ccggcaaacc gatgctacca ctatgataat
    18421 caatatcata tgggttaacg ctgaccagcg gcgcacactc ggtaaggcca tagccttcca
    18481 gcagatactg tcctgtcagt ttcacccaac gctctgccac cacttgctgc actggcatcc
    18541 caccgcctgc ggaaagatgc agactggaga aatccagctg ctggaactct ttattgttca
    18601 gcaacgcatt gaacaaggtg ttaacgccag tgatagcggt aaacggatat ttcgctaact
    18661 cttttaccaa ccctggaata tcacgcgggt tagtgataag caggttctgc ccacccagtt
    18721 cgataaacag caggcagtta atggtcaggg caaaaatgtg atacagcggc agtgccgtta
    18781 ctaccagttc tttacctgga tgcagcaacg gcccataggt ggcgttgacc tgttccaggt
    18841 tcgccagcat attgcggtga gtcagcatcg cgcctttcgc cacacctgtg gtgccgccgg
    18901 tgtattgcag gaaggctaaa tcttccggca ccagttcggg tttgacgtac tgcatccggt
    18961 agccgttatg cagtgcgcta cgaaatgaaa tggcatctgg cagatggtat ttcggcacca
    19021 aacgcttgat gtatttaaca acgaaattga ctaccgtgcc ttttgccgta gatagctgat
    19081 cgcccatacg ggtcagaatt acgtgctgaa cggcggtttt atcaaccact ttttccagtg
    19141 tgtgagcaaa gttagacacg ataacaatcg ccgatgcgcc gctatcgtta agctgatgct
    19201 caagctcacg cggggtatac aacgggttaa cgtttacgac gatcatcccg gcacgcaaaa
    19261 tgccaaacag cgccaccgga tattgcaata aattaggcat catcaacgca acgcgatcgc
    19321 ctttcttcag ccccaaccct tgttgcaaat aagcggcaaa cgcgcgactg cgttcttcca
    19381 gcttgcggaa ggtcattacc tcccccatat tcacaaacgc aggctgatcg gcgtagcgcg
    19441 cgaccgactg ctcaaacata tctaccagag attgataacg gtcagggttg atctccgtcg
    19501 gaacgtccgc gggataacgg ttaagccaaa ccttcttcaa ttcttcacct ctaaaatgcg
    19561 tgttcgtcgt catcgcaacc ccaaatgata tacatgccgt taacataata ttaactcatc
    19621 ataccagctt gataattacc caacgaaaag gttgcgaagc gcgtcactat ttatttttat
    19681 ctttaccgta agaatgcaga aacagcggac cagccgctgt ttctttttct ttaaaaacaa
    19741 gcaattattc agttacaact gtttgtactc tcgcggggcc gggattatac cagccccatc
    19801 cgccgtagcc atagggccag ccacgaccgc cataaaacca tggatcaatc ggctgaggcg
    19861 gcataatcac ctgctgggtt aaatgccaac gtttgtaacc cgttacttgc atcaccataa
    19921 atttataggg cgtgttgccg atttttccgt caaccgcacc agtgattggc ccgactaccg
    19981 taaccagttg tccacggaaa tccaccgggt ccagaaaacc gttcacatcg gcataaatgc
    20041 gaccgcgaga aggttctccc agcgtcggtc tggctccgct gtccagcggt acggtagcaa
    20101 tttccaggcg ggttttccct tgctggtttt gtaccgcaac cactttgcca ccaaagcgtg
    20161 cctcctgacc aacgtacagc tgcggcgcac tcatcacccg aactaaatct tgttgcggcg
    20221 tgggactact gcctttaatg gcgtccggca cagtgacaca accgctcagc attagcgcaa
    20281 acgtacctgc cagtatgcct ttgataacat tcttttgaac cgccatgatg cgactccttt
    20341 ttttctcagg gcatactctt aagattcatt ctttgcccgg aagtttcttc catgcgacgt
    20401 tgttacgtaa ataaaccggt tcggcatgtt ccaccgccac cgttttaccc tcggcaaaca
    20461 tctgacaagc aatcggcagc atatcttctg cagcggggag taacacttcg ccatcgcgca
    20521 aaaccagccc gctctcttta ccgagatccg gccaggcttg ccagcctgta cctaccgtca
    20581 cccattcccc ggaaagctgt tgcattcgtt catgaacgag ttcgggtttg agtacggctt
    20641 cggtttcttc accgtgccag ataccgtttt catcacgctg atattcggcc cagtaaactt
    20701 cgcccattcg cgcgtcaatg gctgacagca cgcgtgttgc gctgtttttg cgccacgccc
    20761 cttgcgccat cgtcattagc gtggagacgc caatcatcgg taattccgcg ccaagcgcca
    20821 gcccttgcgc gatgccaata ccaatgcgca cgccggtaaa gctaccgggg ccgcgcccgt
    20881 aagccagagc gttaatatca gtcagggaag ttccgctggt ggtcaggata tcctgcacca
    20941 tcggtaagat tcgttgagta tgttcacgag ggcaaagctc aaaatgagcg ttgacagtac
    21001 cgtcgttcca cagggcgaca gagcaggcct ctgtcgcggt atcgatagcc agaattcgca
    21061 tgggtcttcg tgcttagatc aataaaaagg cgcgcatcat accatactcc gtaacaaatt
    21121 acctggagga tggtatcgca aggaaacgaa ccgcacgggc aatgtcacgg gtgcgtggcg
    21181 cgggcggcag actggcgaga aacgtcgcgc cgtaaggacg catcaccagc cgattgtcac
    21241 aaattaccaa aaccccgcga tcgtcggcgt cgcgaatcag tcgcccgact ccctgcttga
    21301 gagtaatcac cgcatccggc agttgtactt catcgaacgg gtcgccaccg cgcaaacggc
    21361 aatcttccat gcgcgctttt aacaatggat catcaggcga ggtaaacggc aatttgtcga
    21421 taatcaccaa tgacaatgta tcgccacgca cgtccacccc ctcccagaaa ctactggttg
    21481 ccacaagaag cgcgttaccg gcactgacaa attgctgcaa cagttgccct ttgctggttt
    21541 ccccctgcaa taatacggga agcgtcatgg tagcgcggaa ctgctcggcc agatcgcgca
    21601 tcatggcgtg cgaggtacaa agcataaaac agcgaccgtt gttagcttcg atgatcgggc
    21661 gcagcatagc cgccagttgc cgcgcggaac ccggttgatt ggtttgcggc aggttgcgcg
    21721 gcacacagag taacgcctgg cggctgtaat caaacgggct tggcaatagc aacgactccg
    21781 cctgttctat cccaagccgc gaggtgaaat gatgcagatc gtcgttcacc gacagcgttg
    21841 ctgaggtgaa gatccagcta ccgggctttt gcgccattaa ctctttgaat ttatccgcca
    21901 cgctgagcgg cgtgagagcc agagtaaaat ggcgcgaagt gcattcgtac cagtagctgt
    21961 agcccggctg attgatctct tttagccgct tcagccgtgt gcgatacaac gtggcgcgct
    22021 caaatgccgc atccagcaag gcggaacgcc ccagtgacag tttcgccacg tcataacaaa
    22081 gttccagggt gtcatcgagc agtaaaaatg cccgctgaat ttgcggatta gctaacagct
    22141 cacgcaggtt accgcgataa cctggctcac cgagttgcag acgaaaatcc tgcgcactct
    22201 gggcaagacg atcagcgcac ttttgtaact gctgagtgtc ttttaattcg gtgcggtagg
    22261 cgatggtgat gtcttttgcc aggtcgagca gttgtcgact ggagagtgac tgaccaaaat
    22321 actggctggc aatgtccggt agctggtggg cttcgtcgaa gatcatgacg tccgcttccg
    22381 ggatcaactc gccaaatcca ctctctttaa ccaccatatc cgccagaaag agatgatggt
    22441 ttaccaccac cacatcggcg tccatcgctt ttttacgtgc tttgaccaca aagcaatctt
    22501 tatacatcgg gcagtcgctg ccaagacagt tgtcgttggt gctggtgacc agcggccacg
    22561 cctgcgaatc ttccgccacg ctgacgcagg tgctgatatc accatcgact gtttgattag
    22621 accaggagcg cagcaggatc acatcgctta atatttgtac cggaagatcg ccccccgcca
    22681 gcgcctgctg ttcgagacgt tcgaggcaga ggtagtttga gcgccctttc agcaacgcca
    22741 cgttgcccgt gtatttcaac gcctttgaga ccgtcggcaa atcgcggcta tagagctgat
    22801 cctgcaacgc ttttgagccg gtcgagataa tgactttctt tttcgcccgc agcgcagggg
    22861 ccaggtaagc gtaggttttg cccgtaccgg ttcctgcttc caccaccagc ggctggcctt
    22921 tttctatcgc ctgggtgacg gctaccgcca tctgtcgctg tggttctcgc ggcttaaagc
    22981 ctggtatcgc tttcgccagc tgaccgtctg gtgcaaaatc gtccgtcaca ctaccccctg
    23041 ttgatttgaa cagggattat gtcaggatga gggcgctttc gccagttgaa gtggtgacgg
    23101 cgacctcaca ttgtggcagt ctttgcagca cgaaaatgga aggataatga ggacaaaatg
    23161 atgactatcg ttcgtatcga tgctgaagcc cgctggtctg atgtagtaat ccacaacaac
    23221 acgctctact acactggtgt accggaaaac ctcgacgccg atgcctttga gcaaaccgcc
    23281 aacacgctgg cgcagattga cgccgtgctg gaaaaacagg gcagcaataa atcgagtatt
    23341 ctggatgcca ccattttcct ggccgataaa aacgacttcg cggcgatgaa taaagcgtgg
    23401 gatgcctggg ttgtcgcggg tcatgcgccg gtgcgctgca cggtacaagc gggtttgatg
    23461 aacccgaagt ataaagttga aattaagatt gtggctgcgg tgtaagcttt atcgaagcaa
    23521 aataagtcag acgataattt atcgataata ctggtcggtt ttacataaat cgaccagaga
    23581 ataagatatt actgttgatg cttctgctaa ttcattattt atttttataa tttcaatttt
    23641 atctataaaa ctaactatat aaataaacat aaccatgccg ttgtgttcgc cctttgatta
    23701 gcaactctgg ttttctttct tagctgaaca atctggttat agcgtgcgga ctgaccgtta
    23761 attttctcca tcttactgga taacacacca tgagctgcta cctttaaaac acctgaatgt
    23821 caacaggtta actcgtgcca tatcgttata atccctttga tattagatgc aaattaaggt
    23881 catatatagc cttatcgata acatggttaa ttttaaggat aagaatatgc ctgctgtaat
    23941 agataaagcc ctggatttca ttggtgccat ggatgtatca gcgccaacac caagttcgat
    24001 gaatgaaagc acggcgaagg gaatctttaa atatttaaaa gaactgggag tacccgcgag
    24061 tgccgctgat attacggcgc gagctgacca ggaaggctgg aaccctgggt tcacggaaaa
    24121 aatggttgga tgggcaaaaa aaatggagac aggtgaacgc tctgtgatta aaaatcctga
    24181 atacttttca acatatatgc aggaagaact aaaagcactg gtctgagtta aatttatatc
    24241 agcataaatg ggtcaggacg cttttaatca catataaaaa gcgtcctttt tctcacaatc
    24301 aaataatcat cggcttctat aacgaaatcg atacggatta gtgtgttact cgtcttcatc
    24361 ctcaaaacgc gccacgattc gctcgccgga gtggttggca cgcagctctt ctgccaccag
    24421 cgcgattgcc tggccgctgc tcatcccttg tgccatcagt tcctggatcc gctcgacagc
    24481 tttttgctgc tgttcatggg tgagtgaagg taaacctgca aacattgtta actcctgcta
    24541 aattgttggc gctaattatt tcatgctacc cggcacatag ccagtagagt caggactgat
    24601 gaagacgtta tctcccgctg tgattacttt accctggcgt caggacgccg ctgaatttta
    24661 tttctcccgc ttaagccacc tgccgtgggc gatgctttta cactccggct atgccgatca
    24721 tccgtatagc cgctttgata ttgtggtcgc cgagccgatt tgcactttaa ccactttcgg
    24781 taaagaaacc gttgttagtg aaagcgaaaa acgcacaacg accactgatg acccgctaca
    24841 ggtgctccag caggtgctgg atcgcgcaga cattcgccca acgcataacg aagatttgcc
    24901 atttcagggc ggcgcactgg ggttgtttgg ctacgatctg ggccgccgtt ttgagtcact
    24961 gccagaaatt gcggaacaag atatcgttct gccggatatg gcagtgggta tctacgattg
    25021 ggcgctcatt gtcgaccacc agcgtcatac agtttctttg ctgagtcata atgatgtcaa
    25081 tgcccgtcgg gcctggctgg aaagccagca attctcgccg caggaagatt tcacgctcac
    25141 ttccgactgg caatccaata tgacccgcga gcagtacggc gaaaaatttc gccaggtaca
    25201 ggaatatctg cacagcggtg attgctatca ggtgaatctc gcccagcgtt ttcatgcgac
    25261 ctattctggc gatgaatggc aggcattcct tcagcttaat caggccaacc gcgcgccatt
    25321 tagcgctttt ttacgtcttg aacagggtgc aattttaagc ctttcgccag agcggtttat
    25381 cctttgtgat aatagtgaaa tccagacccg accgattaaa ggcacgctac cacgcctgcc
    25441 cgatcctcag gaagatagca aacaagcaga aaaactggcg aactcagcga aagatcgtgc
    25501 cgaaaatctg atgattgtcg atttaatgcg taatgatatc ggtcgtgttg ccgtagcagg
    25561 ttcggtaaaa gtaccagagc tgttcgtggt ggaacccttc cctgccgtgc atcatctggt
    25621 cagcaccata acggcgcaac taccagaaca gttacacgcc agcgatctgc tgcgcgcagc
    25681 ttttcctggt ggctcaataa ccggggctcc gaaagtacgg gctatggaaa ttatcgacga
    25741 actggaaccg cagcgacgta atgcctggtg cggcagcatt ggctatttga gcttttgcgg
    25801 caacatggat accagcatta ctatccgcac tctgactgcc attaacggac aaatatactg
    25861 ctctgcgggc ggtggaattg tcgccgatag ccaggaagaa gcggaatatc aggaaacttt
    25921 tgataaagtt aataagatat tacgccaact ggagaagtaa gacgtggaat accgtagcct
    25981 gacgcttgat gattttttat cgcgctttca acttttgcgc ccgcaaatta accgggaaac
    26041 cctaaatcat cgtcaggctg ccgtgttaat ccccatcgtc cgtcgaccgc aaccggggtt
    26101 gttgctgact cagcgttcaa ttcatctgcg taaacacgct ggacaagtgg cattccctgg
    26161 aggtgcagtc gatgacacgg acgcatcagt tatcgccgcc gcgctgcgcg aagctgaaga
    26221 agaggttgct ataccgcctt ccgccgttga agttatcggc gtgctgccgc ccgtcgatag
    26281 cgtaactggc taccaggtaa ccccagtggt cggcattatc ccgcccgatc tgccgtatcg
    26341 cgccagtgaa gatgaagtct cggcggtgtt tgaaatgccg ctcgcccagg cattacatct
    26401 gggtcgttat caccctttag atatctaccg ccgtggcgat tcacatcggg tatggctgtc
    26461 ctggtacgaa cagtattttg tatggggaat gaccgcaggc ataattcgtg agctggcgct
    26521 gcaaattggt gtgaaaccct gactatactt atctttacat ctacaaaaca ctacttgaga
    26581 caatcatcgc aatattagtt aaatcgcggt ttttgattag tttaattcat gtgaatagtt
    26641 aagccagtcg ccgcgttccc tcttacacta tgcgctgtta ttagttcgtt actggaagtc
    26701 cagtcacctt gtcaggagta ttatcgtgat tagtctattc gacatgttta aggtggggat
    26761 tggtccctca tcttcccata ccgtagggcc tatgaaggca ggtaaacagt tcgtcgatga
    26821 tctggtcgaa aaaggcttac tggatagcgt tactcgcgtt gccgtggacg tttatggttc
    26881 actgtcgctg acgggtaaag gccaccacac cgatatcgcc attattatgg gtcttgcagg
    26941 taacgaacct gccaccgtgg atatcgacag tattcccggt tttattcgcg acgtagaaga
    27001 gcgcgaacgt ctgctgctgg cacagggacg gcatgaagtg gatttcccgc gcgacaacgg
    27061 gatgcgtttt cataacggca acctgccgct gcatgaaaac ggtatgcaaa tccacgccta
    27121 taacggcgat gaagtcgtct acagcaaaac ttattattcc atcggcggcg gttttatcgt
    27181 cgatgaagaa cactttggtc aggatgctgc caacgaagtg agcgtgccgt atccgttcaa
    27241 atctgccacc gaactgctcg cgtactgtaa tgaaaccggc tattcgctgt ctggtctcgc
    27301 tatgcagaac gaactggcgc tgcacagcaa gaaagagatc gacgagtatt tcgcgcatgt
    27361 ctggcaaacc atgcaggcat gtatcgatcg cgggatgaac accgaaggtg tactgccagg
    27421 cccgctgcgc gtgccgcgtc gtgcgtctgc cctgcgccgg atgctggttt ccagcgataa
    27481 actgtctaac gatccgatga atgtcattga ctgggtaaac atgtttgcgc tggcagttaa
    27541 cgaagaaaac gccgccggtg gtcgtgtggt aactgcgcca accaacggtg cctgcggtat
    27601 cgttccggca gtgctggctt actatgacca ctttattgaa tcggtcagcc cggacatcta
    27661 tacccgttac tttatggcag cgggcgcgat tggtgcattg tataaaatga acgcctctat
    27721 ttccggtgcg gaagttggtt gccagggcga agtgggtgtt gcctgttcaa tggctgctgc
    27781 gggtcttgca gaactgctgg gcggtagccc ggaacaggtt tgcgtggcgg cggaaattgg
    27841 catggaacac aaccttggtt taacctgcga cccggttgca gggcaggttc aggtgccgtg
    27901 cattgagcgt aatgccattg catctgtgaa ggcgattaac gccgcacgga tggctctgcg
    27961 ccgcaccagt gcaccgcgcg tctcgctgga taaggtcatc gaaacgatgt acgaaaccgg
    28021 taaggacatg aacgccaaat accgcgaaac ctcacgcggt ggtctggcaa tcaaagtcca
    28081 gtgtgactaa tacttcttac tcgcccatct gcaacggatg ggcgaattta tccccgcttt
    28141 ctcgtctgct gtaatattcc ccactacact tccactgttg cgtcaggcgt ttgtcgccat
    28201 acgcttacag ggtggcccgc atgcaaaaag cacaacggat cattaaaacc tatcgccgta
    28261 atcgaatgat tgtttgtacg atttgcgcac tcgttacgct cgcttcgacc ctgagcgtgc
    28321 gatttatttc acagcgtaac ttaaatcaac aacgggtagt acaattcgcc aatcatgctg
    28381 tagaggaatt agataaagta ctgcttcccc tacaggcagg tagcgaagtc ttgcttccgc
    28441 tgattggtct gccctgctct gtcgcccatt tgccattacg taaacaggcg gcaaaactcc
    28501 aaactgtgcg atccattggc ctggtgcaag acggcacact ctattgctcc agcatttttg
    28561 gttatcgcaa tgtgcccgtc gtggacattc tggctgaact tcctgcaccg caaccacttt
    28621 tacgcctgac gatcgaccgt gccctgatta aaggcagtcc ggttttgatt caatggacgc
    28681 ctgcagcggg cagtagcaat gctggggtca tggagatgat taacatcgac ttactgacgg
    28741 caatgctcct tgagccacaa ctgccgcaaa tcagtagcgc cagcctgacg gtggacaaac
    28801 ggcatttgct ctatggtaat gggctggtag attcccttcc gcaacctgaa gacaatgaaa
    28861 actatctggt ttcttcgcaa cgctttcctt ttaccattaa cgttaatggt ccgggggcta
    28921 cggcgctggc atggcactat cttccaacac aattaccgct ggcggtgctg ctaagtttac
    28981 tggtgggcta catcgcctgg ctggcgaccg cttaccggat gagcttttcc cgcgaaatca
    29041 atctgggcct ggcgcaacat gagttcgaat tgttctgtca gcctttgctt aatgcgcgca
    29101 gccagcaatg tattggtgta gagattttgc tgcgctggaa caatccgcgt cagggctgga
    29161 tttcaccgga tgtgtttatt cctatcgcgg aagaacatca tttaattgtg ccactgaccc
    29221 gctatgtgat ggcagaaacc attcgtcagc gccatgtttt cccgatgagt agtcagtttc
    29281 atgttggcat taacgtcgca cccagccatt ttcgccgtgg tgtgctgata aaagatctca
    29341 atcagtactg gtttagcgct cacccgattc agcaactgat cctcgaaatc accgaacgcg
    29401 atgccttact ggatgttgat tatcggattg cccgcgagct gcatcgtaaa aatgtcaaac
    29461 tggcgattga tgacttcggc accggcaaca gttcattttc ctggcttgaa acattacgtc
    29521 ctgacgtgct gaaaattgat aagtcattta ccgcagctat aggttctgac gcggttaact
    29581 cgacggtgac cgatatcatc atcgcgctgg ggcaaagact gaatattgaa ctggtggcgg
    29641 agggcgtgga aacgcaagaa caagcgaagt atttgcgccg tcatggcgtg catattttgc
    29701 aagggtattt gtacgcacag ccgatgccgc tacgtgattt tcccaaatgg ctggcgggca
    29761 gccaaccgcc gcccgcccgg cataatggac atatcacgcc cattatgccg ttacgttaac
    29821 gcttactcat cttcatcgtg cgccggttgc tctttaacaa tgcgaaccag atcaacacga
    29881 taatcattgg cttcaatgat ggtgatatgc agtggcccta catcaatcac atcgcccaca
    29941 cggggaatgt gaccatttgc cgagatcacg aggcccgcga ccgtcgcgat atcatcgtca
    30001 tcggcaaggt gctcaacatc aagcgcctgc tgcaaggcat gcaaatctgt accgcctttt
    30061 accagccagc cgtcaccatc agtaatgatt tccggcgttt cgtcagcgtc cgggaattca
    30121 cccgcaatgg cttccagcac atccagcggc gtgaccaagc cttgtaccac accaaactcg
    30181 ttggtcacga taacaaagct cccgcgagca cgacgcagca cgcccagcag gttgatcgga
    30241 tcgagggttt cagggacgat aatcgccgga gacgccgaag caatcgccgc cacatcaacg
    30301 ccctcttcca gcgccaccag cagttcttta gcacgtacga ttccgatgat ttcatccagt
    30361 tcaccgcgac ataccgggaa caggctgtgc ggtgaggaga gcagctgctc gcggatttca
    30421 tcgaccccga gattagcgtc aacccagctt atttcaccgc gcggcgtcat gatcccgcgc
    30481 agagaacgcg acgccagcgt cagtacgccg ttaatcatgt aacgttcttc ttcggcaaat
    30541 gcaccttccg ggatcggcat cggcatcggg ttatcggcat cgtgctgaac attggcctga
    30601 cgtttcccgc ccatcaaacg caggatggca tcggcagtac gcgctcgcag cggcaaagtc
    30661 gactggtgac gaataaagtt gcgacgcgca atctggttaa acacttcgat gatgatcgag
    30721 aagccaatcg cggcatacag gtaacctttc ggaatgtgga aaccgaaacc ttctgccacc
    30781 agactcagac caatcattaa caggaagctc agacagagca ccaccaccgt ggggtgctgg
    30841 ttaacaaatc gcgtcagcgg tttggatgcc agcaacataa ccgccatcgc aatcactacc
    30901 gccgccatca tcaccggcag atggttaacc atccctacag cagtaattac cgcatccaac
    30961 gagaagacgg cgtcaaggat gacaatctgt gtgacgacta cccagaaact ggcgtagcct
    31021 ttaccgtggc cggaatcatg atcgcggttt tccagccgtt catgcagttc ggttgttgct
    31081 ttgaacagca agaatatccc cccgaacaac ataatcaggt cgcgtccgga gaaggagaaa
    31141 tccatgacgg taaatagcgg tttggtcagc gtgaccatcc atgaaatcag cgacagcagc
    31201 cccagacgca taatcagcgc cagtgataac cccagcaaac gcgctttatc gcgttgtttt
    31261 ggcggcagtt tgtcagcaag aatggcgatg aagaccaggt tatcgatacc cagcacaatt
    31321 tcgagaacaa caagcgtgag tagccccgcc caaattgagg ggtccattaa gaattccatg
    31381 acaagctcct gcttaaggaa tagctattcg acgccagaaa taatgcaggc gtaacgacaa
    31441 aatgcaaacg aaaggtgcgg catagagtgc cagaaaggca ggcgttaaaa ggcctgatgc
    31501 tgaaatgacg tcggtgacga tccatactgc gggctactgc cctatactcc atggttgtta
    31561 aacgggagtt aaacatatca gagacgcctc tgatttggca aagatttacc ttcctttgca
    31621 aacgaatgtg acaaggatat tttacctttc gaaatttctg ctaatcgaaa gttaaattac
    31681 ggatcttcat cacataaaat aattttttcg atatctaaaa taaatcgcga aacgcagggg
    31741 tttttggttg tagcccttat ctgaatcgat tcgattgtgg acgacgattc aaaaatacat
    31801 ctggcacgtt gaggtgttaa cgataataaa ggaggtagca agtgaccatt gctattgtta
    31861 taggcacaca tggttgggct gcagagcagt tgcttaaaac ggcagaaatg ctgttaggcg
    31921 agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga aaatgccgaa acgctgattg
    31981 aaaagtacaa cgctcagttg gcaaaactcg acaccactaa aggcgtgctg tttctcgttg
    32041 atacatgggg aggcagcccg ttcaatgctg ccagccgcat tgtcgtcgac aaagagcatt
    32101 atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga aacgttaatg gctcgtgatg
    32161 atgacccaag ctttgatgaa ctggttgcgc tggcagtaga aacaggccgt gaaggcgtga
    32221 aagcactgaa agccaaaccg gttgaaaaag ccgcgccagc acccgctgcc gcagcaccaa
    32281 aagcggctcc aactccggca aaaccaatgg ggccaaacga ctacatggtt attggccttg
    32341 cgcgtatcga cgaccgtctg attcacggtc aggtcgccac ccgctggacc aaagaaacca
    32401 atgtctcccg tattattgtt gttagtgatg aagtggctgc ggataccgtt cgtaagacac
    32461 tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt agttgatgtt gccaaaatga
    32521 ttcgcgtcta caacaacccg aaatatgctg gcgaacgtgt aatgctgtta tttaccaacc
    32581 caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat cacctctgtt aacgtcggtg
    32641 gtatggcatt ccgtcagggt aaaacccagg tgaataacgc ggtttcggtt gatgaaaaag
    32701 atatcgaggc gttcaaaaaa ctgaatgcac gcggtattga gctggaagtc cgtaaggttt
    32761 ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa aatcgataag taacgtattg
    32821 tgttgattat cactcagttt tcacacttta gtcttacgta aacaggagaa gtacaatgga
    32881 gattaccact cttcaaattg tgctggtatt tatcgtagcc tgtatcgcag gtatgggatc
    32941 aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg tgtaccctgg tgggtatcgt
    33001 tcttggggat atgaaaaccg gtattattat cggtggtacg ctggaaatga tcgcgctggg
    33061 ctggatgaac atcggtgctg cagttgcgcc tgacgccgct ctggcatcta tcatttctac
    33121 cattctggtt atcgcaggtc atcagagcat tggtgcaggt attgcactgg caatccctct
    33181 ggccgctgcg ggccaggtac tgaccatcat cgttcgtact attaccgttg ctttccagca
    33241 cgctgcggat aaggctgctg ataacggcaa cctgacagcg atttcctgga tccacgtttc
    33301 ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc gtcatcgttg cgctgtctgt
    33361 aggtaccagc gaagtacaga acatgctgaa tgcgattccg gaagtggtga ccaatggtct
    33421 gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg atggttatca acatgatgcg
    33481 tgctggctac ctgatgccgt tcttctacct cggcttcgta accgcagcat tcaccaactt
    33541 taacctggtt gctctgggtg tgattggtac tgttatggca gtgctctaca tccaacttag
    33601 cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggca gctggtaaca acgatctcga
    33661 taacgaactg gactaacagg tgagcgaaat ggttgataca actcaaacta ccaccgagaa
    33721 aaaactcact caaagtgata ttcgtggcgt cttcctgcgt tctaacctct tccagggttc
    33781 atggaacttc gaacgtatgc aggcactggg tttctgcttc tctatggtac cggcaattcg
    33841 tcgcctctac cctgagaaca acgaagctcg taaacaagct attcgccgtc acctggagtt
    33901 ctttaacacc cagccgttcg tggctgcgcc gattctcggc gtaaccctgg cgctggaaga
    33961 acagcgtgct aatggcgcag agatcgatga cggtgctatc aacggtatca aagtcggttt
    34021 gatggggccg ctggctggtg taggcgaccc gatcttctgg ggaaccgtac gtccggtatt
    34081 tgcagcactg ggtgccggta tcgcgatgag cggcagcctg ttaggtccgc tgctgttctt
    34141 catcctgttt aacctggtgc gtctggcaac ccgttactac ggcgtagcgt atggttactc
    34201 caaaggtatc gatatcgtta aagatatggg tggtggcttc ctgcaaaaac tgacggaagg
    34261 ggcgtctatc ctcggcctgt ttgtcatggg ggcattggtt aacaagtgga cacatgtcaa
    34321 catcccgctg gttgtctctc gcattactga ccagacgggc aaagaacacg ttactactgt
    34381 ccagactatt ctggaccagt taatgccagg cctggtacca ctgctgctga cctttgcttg
    34441 tatgtggcta ctgcgcaaaa aagttaaccc gctgtggatc atcgttggct tcttcgtcat
    34501 cggtatcgct ggttacgctt gcggcctgct gggactgtaa gactgttgta cactaccggg
    34561 gccttttggc cccgtttttt tatctggagg attaatgaca atcacggacc tggtactgat
    34621 tcttttcatc gccgcactcc tggccttcgc gatctacgat cagttcatca tgccccgccg
    34681 taacggcccc accctgctgg caattccttt gctccggcgt ggtcgcatcg atagcgttat
    34741 cttcgtcgga ttgattgtca ttcttatcta taacaacgtc acgaatcatg gtgcgttaat
    34801 aacgacatgg ttattaagcg cactggctct gatgggtttt tatatattct ggatccgcgt
    34861 tccgaagatc atctttaaac aaaaaggttt tttcttcgcc aatgtctgga ttgaatatag
    34921 ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg gtgatgcaat tagaacagcg
    34981 tcggctgtta atccgcgttc gaaatatcga cgatctggaa agaatttata aacttctcgt
    35041 ttcaactcaa tgagttatta atttagccaa agctatgttt agtgtatttt taataatcag
    35101 acatagctta ggctatatta
//
LOCUS       DAZFFG010000034        35088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFFG010000034 DAZFFG010000000
VERSION     DAZFFG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.034"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(52..417)
                     /gene="yhaH"
                     /locus_tag="ACHJ7J_002085"
     CDS             complement(52..417)
                     /gene="yhaH"
                     /locus_tag="ACHJ7J_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5000322.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(710..1696)
                     /gene="yqjG"
                     /locus_tag="ACHJ7J_002086"
     CDS             complement(710..1696)
                     /gene="yqjG"
                     /locus_tag="ACHJ7J_002086"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO5000323.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYSFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1766..2158)
                     /gene="yqjF"
                     /locus_tag="ACHJ7J_002087"
     CDS             complement(1766..2158)
                     /gene="yqjF"
                     /locus_tag="ACHJ7J_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5000324.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2344..2643)
                     /gene="yqjK"
                     /locus_tag="ACHJ7J_002088"
     CDS             complement(2344..2643)
                     /gene="yqjK"
                     /locus_tag="ACHJ7J_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO5000325.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2633..3037)
                     /gene="yqjE"
                     /locus_tag="ACHJ7J_002089"
     CDS             complement(2633..3037)
                     /gene="yqjE"
                     /locus_tag="ACHJ7J_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5000326.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3040..3345)
                     /gene="yqjD"
                     /locus_tag="ACHJ7J_002090"
     CDS             complement(3040..3345)
                     /gene="yqjD"
                     /locus_tag="ACHJ7J_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO5000327.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3383..3751)
                     /gene="yqjC"
                     /locus_tag="ACHJ7J_002091"
     CDS             complement(3383..3751)
                     /gene="yqjC"
                     /locus_tag="ACHJ7J_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO5000328.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQQRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3898..4281)
                     /gene="mzrA"
                     /locus_tag="ACHJ7J_002092"
     CDS             complement(3898..4281)
                     /gene="mzrA"
                     /locus_tag="ACHJ7J_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO5000329.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4285..4947)
                     /gene="yqjA"
                     /locus_tag="ACHJ7J_002093"
     CDS             complement(4285..4947)
                     /gene="yqjA"
                     /locus_tag="ACHJ7J_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO5000330.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4969..5052)
                     /gene="yqiM"
                     /locus_tag="ACHJ7J_002094"
     CDS             complement(4969..5052)
                     /gene="yqiM"
                     /locus_tag="ACHJ7J_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO5000331.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5292..6068)
                     /gene="exuR"
                     /locus_tag="ACHJ7J_002095"
     CDS             complement(5292..6068)
                     /gene="exuR"
                     /locus_tag="ACHJ7J_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO5000332.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6198..7496)
                     /gene="exuT"
                     /locus_tag="ACHJ7J_002096"
     CDS             complement(6198..7496)
                     /gene="exuT"
                     /locus_tag="ACHJ7J_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO5000333.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7979..9391
                     /gene="uxaC"
                     /locus_tag="ACHJ7J_002097"
     CDS             7979..9391
                     /gene="uxaC"
                     /locus_tag="ACHJ7J_002097"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO5000334.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9406..10893
                     /gene="uxaA"
                     /locus_tag="ACHJ7J_002098"
     CDS             9406..10893
                     /gene="uxaA"
                     /locus_tag="ACHJ7J_002098"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO5000335.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10976..11527
                     /gene="ygjV"
                     /locus_tag="ACHJ7J_002099"
     CDS             10976..11527
                     /gene="ygjV"
                     /locus_tag="ACHJ7J_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO5000336.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11532..12776)
                     /gene="sstT"
                     /locus_tag="ACHJ7J_002100"
     CDS             complement(11532..12776)
                     /gene="sstT"
                     /locus_tag="ACHJ7J_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO5000337.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13099..14064)
                     /gene="alx"
                     /locus_tag="ACHJ7J_002101"
     CDS             complement(13099..14064)
                     /gene="alx"
                     /locus_tag="ACHJ7J_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO5000338.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(14348..15334)
                     /gene="ygjR"
                     /locus_tag="ACHJ7J_002102"
     CDS             complement(14348..15334)
                     /gene="ygjR"
                     /locus_tag="ACHJ7J_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5000339.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQAQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARDNQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKQ"
     gene            complement(15413..16105)
                     /gene="ygjQ"
                     /locus_tag="ACHJ7J_002103"
     CDS             complement(15413..16105)
                     /gene="ygjQ"
                     /locus_tag="ACHJ7J_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO5000340.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATLFIADRVMINAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(16182..16685)
                     /gene="ygjP"
                     /locus_tag="ACHJ7J_002104"
     CDS             complement(16182..16685)
                     /gene="ygjP"
                     /locus_tag="ACHJ7J_002104"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO5000341.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16770..17906
                     /gene="rlmG"
                     /locus_tag="ACHJ7J_002105"
     CDS             16770..17906
                     /gene="rlmG"
                     /locus_tag="ACHJ7J_002105"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO5000342.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            complement(18017..20035)
                     /gene="fadH"
                     /locus_tag="ACHJ7J_002106"
     CDS             complement(18017..20035)
                     /gene="fadH"
                     /locus_tag="ACHJ7J_002106"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO5000343.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMLILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGEPQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20470..22821)
                     /gene="ygjK"
                     /locus_tag="ACHJ7J_002107"
     CDS             complement(20470..22821)
                     /gene="ygjK"
                     /locus_tag="ACHJ7J_002107"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO5000344.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQIQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGALKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22838..23908)
                     /gene="ygjJ"
                     /locus_tag="ACHJ7J_002108"
     CDS             complement(22838..23908)
                     /gene="ygjJ"
                     /locus_tag="ACHJ7J_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO5000345.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            complement(24042..25475)
                     /gene="ygjI"
                     /locus_tag="ACHJ7J_002109"
     CDS             complement(24042..25475)
                     /gene="ygjI"
                     /locus_tag="ACHJ7J_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5000346.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25538..25987)
                     /gene="ebgC"
                     /locus_tag="ACHJ7J_002110"
     CDS             complement(25538..25987)
                     /gene="ebgC"
                     /locus_tag="ACHJ7J_002110"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO5000347.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25984..29076)
                     /gene="ebgA"
                     /locus_tag="ACHJ7J_002111"
     CDS             complement(25984..29076)
                     /gene="ebgA"
                     /locus_tag="ACHJ7J_002111"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO5000348.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGKRYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASHKFGAGFFSTNLHSENKQ
                     "
     gene            complement(29260..30243)
                     /gene="ebgR"
                     /locus_tag="ACHJ7J_002112"
     CDS             complement(29260..30243)
                     /gene="ebgR"
                     /locus_tag="ACHJ7J_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO5000349.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30462..30794
                     /gene="ygjH"
                     /locus_tag="ACHJ7J_002113"
     CDS             30462..30794
                     /gene="ygjH"
                     /locus_tag="ACHJ7J_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO5000350.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(30836..32215)
                     /gene="ygjG"
                     /locus_tag="ACHJ7J_002114"
     CDS             complement(30836..32215)
                     /gene="ygjG"
                     /locus_tag="ACHJ7J_002114"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO5000351.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32633..34153
                     /gene="aer"
                     /locus_tag="ACHJ7J_002115"
     CDS             32633..34153
                     /gene="aer"
                     /locus_tag="ACHJ7J_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO5000352.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(34260..34883)
                     /gene="nfeR"
                     /locus_tag="ACHJ7J_002116"
                     /gene_synonym="yqjI"
     CDS             complement(34260..34883)
                     /gene="nfeR"
                     /locus_tag="ACHJ7J_002116"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO5000353.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
BASE COUNT         8508 a         9173 c         9168 g         8239 t
ORIGIN      
        1 aacatgatga taaaagccgg atgagtcaga catccggcga gcagatttgt tttaaggttc
       61 cagttttgga tcaggtccaa agcggttttc gcctggcgta cccgcctggc agttaaagac
      121 gatgataata agccagccga taaacgggat taagaatagc agtgcccacc acgccgagcg
      181 gtcggtgtcg tgcaggcggc ggaactgaac tgcccaccac ggtaaaaaca ccagaatacc
      241 gtagattgtc gtcaggatgc cttcgccacc agcgcgttgc cagcctaaca ttttatccag
      301 cagccccagc acgaacgtaa agatgatgtt gaccagaata aacatccagt actctttgcg
      361 ccgcgcacga ccccggaaac cgacataatt ttttagtact ttcagatacc agtccatttt
      421 tgcctcaatt ttgctcaatc acttgttttt aaagcagcaa attaagaata gacgtgaatg
      481 aaatggcagt aaatattcgt tcaaggaatt aataaagtgc tgtgaaggcg cgctaagact
      541 gatgacaagc gcaaaattgc cagatgcgct acgcttatct agcctgcgcg gtcattgcaa
      601 catattgaat ataaaagttt tgtaggtttg ataaggcgtt catgccgcat ccgacatgaa
      661 caacatgcac ttagctaatt atcagaggca cctcgtaagg tgcctttttt taaccgaagc
      721 gaacatctcg tccatgcggt tcatcgagat cctgccacgg accaattgaa ataatccccg
      781 tagggttgat ggtcttatga ctgcggaagt aatgattacg gatatgatcg aaattgactg
      841 tttcggcaat tcccggcatc tggtagatat cacgcaggaa gctatacaga ttaaggtaat
      901 cgctgatgcg gtgcttatca cacttgaagt gggtcacata cactggatca aaacggacca
      961 gcgtggtcca caggcgaata tcggcttcgg ttagctggtt gccggtcagg taacgatgct
     1021 gacctaaaat ctgttccagt cgcgccagcg attcaaacac tttctccacc gcttcatcgt
     1081 aggcttgctg gctggtggca aaaccagctt tatacacgcc gttgttaacg gtgtcataaa
     1141 tccagccgtt aagttcgtca attttcgttt gcagggctgg tgggtagtaa tcacccgctt
     1201 tcgcgcccag cgcatcaaac gcggtattaa acatgcggat gatttctgct gattcgttgc
     1261 tgacgatggt gtggttcttt ttgtcccaca gcacgggaac agtaactcgt ccgctgtagt
     1321 gtggatcggc gtggagataa agctgataca aaaattcatg ttgatagagc gtgtctccgg
     1381 ttgctcccgg aaaactgtcg tcaaaggtcc agccgttttc cagcatcagc gggttcacta
     1441 cggaaacgga aataaacggt tccagtcctt tgagtttgcg catgatcagc gtgcggtgcg
     1501 cccacgggca ggcgagtgaa acatagagat gataacgatc tttctctgcg ataaaaccgc
     1561 ctgtgccagt ggggcctgga gcgccatcgg cagtgagcca gttacgaaat gcggaagctg
     1621 aacgttgaaa tttaccgccg gtagatttgg tatcgtacca ggtgtcatgc cagacgccgt
     1681 caatcagttg acccattttt ctctcctcca atagcaaaaa cgaggagctt cctcctcgtt
     1741 ttttagcatt aagtatagcg cgtgcttacc actttttatt cagcaggcgg tcgatgctat
     1801 acgcgcccgg accggtaatt gccagcagca ggaatccgcc agaaattgtc aggtttttca
     1861 tgaacatcag cgagttgacg ccttcagcaa agttgctgtg aaataaaaat gccgtcagca
     1921 gcgtaaagcc cgcagtaaac agggctgtgg tgcgagtcag gaaaccgaac aggattgcca
     1981 gaccaccacc aaactcaagc agaatcacca gcggcagcat aaaacccggg acgcccatcg
     2041 cttccatata ttgttgggta cccgcgtaac cagtaatttt tccccagcct gcggtaataa
     2101 acagaatcgg cattaaaatg cgcgctacca gtacaccaac atcttctaat tttttcatca
     2161 tactctccac tgcgccactc aggctgctga ttgttttttt gtgcaagggc gcggtattag
     2221 cgtcgttgct gtcgatggag agaatcataa acgtggtgaa tgatgattgt tagcaaggaa
     2281 aactgtcaaa aatcttcaaa aaatttgatg ggtaaggccg gaatggctcc ggccagaggg
     2341 aagttaaccg cgaagctgtt gctgcttgag ggtcgttttc accagacgcc aggcgctcca
     2401 tacgccaaaa ccgcgtctgg cccagcggac cagcatatta ggatggcgaa tcgtccagat
     2461 cgccatcacg ctactgccaa ccagcgccca ggagcgcaga cttagcagca tattccagcg
     2521 acgatcgtaa gcgcctgttg cctccagcca ttcacgacga ctggcagaaa gatccagccg
     2581 ttgttgctgg atctggctaa gcagttgcgc cttacgtcgt tcacgttcga ctttactgct
     2641 cacgggactc ctcctcgagc agctgccgat cgtttgccag ctcatggcgt gtatggcgca
     2701 gcaaggtgga tttacgcgat ttacgtaacg tccagatacc gccaatcagt gccagtagca
     2761 gcaacaccac ggtggtggca atcatcgcat tcaggcgata ttgcggatca accgcccaaa
     2821 taattagcac catcaggctc ataagaccaa atgcagcgaa aagcatcgtc aggcccagca
     2881 tcagtaaaag ttgaaagaga ttagcttttt cctcttccag ctcaaccacc gccagccgca
     2941 gacgtgtttc caccatttca accatgatgg aaacaattcg ctgcccgatg cccagaacgc
     3001 ttttaccggg cccttgcgcg tgatgagtgt ccgccataat taacgacgcg acagcagaac
     3061 gccgagcact acaccgattg cagcgccaat gcccacgccc gtccatggat tttcacgcac
     3121 atactcatcg gcacgcgccg ccgcgacacg ggtttgtttg gcgatggcat caccggtttc
     3181 acccaggcga taacggctct gtttcagtgc ctgctccgct ttgctacgaa tcttactcaa
     3241 ctcttctttc gacttctcgc cagatgagct aagcacctct tccagcgtat cggaaaggga
     3301 tttcaactca gcacgcagat gttccgtagt gtgttctttc gacatagttt tctccaggtg
     3361 agtaagtagt gactattgtg agttagtagt cgcgcgcttc cagctttttc agctcttcct
     3421 gcgcttctgc cagtttccgt tcgcgtttgg caatcttatc ggcatcgcct ttttgcttcg
     3481 cctcggctaa atcttgctgg cgttccgcca cctcatcttt ctgctttgcg attttcttct
     3541 gatgatcggc acgctgctgg ctatctgaac agttggcccg gacttcactc agggctttat
     3601 tcagaccgtc aatacgattc tggttttggt gtttttcggc atagctgatc tccttaagga
     3661 tattttgctc cttttcctga cacagggtag tggcataact accggcacta agagcaaaaa
     3721 gagaaacagc taaagcgatg cggtatttca ttcttgaacc ttccattgtg attcggacct
     3781 attacaaagg aacgattgtc cagacaacga agcggtagtg ttaaccaccg cctcattaat
     3841 catagacagt tacggtgaaa tcaccaaagt gagtgatctg tttcggataa caggaagtta
     3901 tccgaagcga tgagagttat cccgtagccg ggtcagccac tgcatagcct gactgttatt
     3961 gtcctgctgc gcaatgatgt agccttgggg caatgttctg tcgaggaccg cttttgcagc
     4021 ggcgctctgg tcgctggagt caaaggtaat taacagagtg tcgtttttgg gggtgatact
     4081 tttgaaagga atgccatgag cgtcaaggtg atgccagatt gaaaaaccgt ctggcatcgt
     4141 tgtgccttga tgaacggcac gaatcgccag cgtagactct tgctggcgaa ccgcggacca
     4201 ggccagcaac agcgtgccga ccagaagtaa aacagcgccg gaccaggcta gctggcgaag
     4261 cgacatgcga ggtatttgca tcccttaccc ccgatttcca tatttctttt tccataacac
     4321 gaccagagaa cctgccaggc caaacaccag cagcaccacc gggagcagca tcagacatga
     4381 catcagctgg tcctcgtact ttaaaaatac cggcgttttg ccgagcatgt aacccagagt
     4441 tgtcaggatc aatacccaca gcagaccgct catccagttg aaaaactgaa agcgcgcgtt
     4501 attcagccct gataacccgg caatcgtcgg cagcagtgtt ctgacaaacg caataaagcg
     4561 accaattaac agcgccgata aaccgtgttt atgaaaaaga tggtgtgcgc gttgatgata
     4621 atgcgcgggt aaatgagata gccagttttg tacggtgcgg gtattgccca gccatcgccc
     4681 ctgaatatag ctgacccagc agccgaggct ggcggcaacg gtcagcagca gaatcgtttg
     4741 cggatagccc atcgcgcctt tcgcaatcaa cacgccgacc aataccagta aactgtcgcc
     4801 cggtaaaaag gccgccggaa gcaagccgtt ttcaaggaac aaaattacaa acaagacaaa
     4861 atacaacatg ccaatcatcg atggattggc cagggtttca aaatcctgcg cccacagggc
     4921 ttgcagcaat tgggtcaaaa gttccattca ttattcctgg tgaatcggtt aacgccacgc
     4981 tggttttaaa agatgcacta ccgcaatgtt attgctgttt tggtatgttc tgcgggtttg
     5041 atacatcaac acagcctgaa ctattccctg ttaaatcgtg agataagaaa gcattcaaaa
     5101 acataaattc aaattgaatc taattgtaac aaaggtcaac gttgttcgtt atcaaaatta
     5161 catgtccgtg gagtctgatt ttgctgaatg caggggaggg tggcgagagg atttacacag
     5221 gctgacactt tatacttttc gcttaccgac gagacaaaca ggcgcattgc agcgcctgtc
     5281 ggacaggaat ttcatttact gccgctggtg gctgtatcga gatgcaccac cgggttttcg
     5341 gcgaacagat aacggtcggc attgaactcg aagtcgtcgc tggtttcgtt aaataacatg
     5401 atcttggtgt tttccaggtg ctgccacatt gccagcttag cagcatgagg atctttgcga
     5461 atcagcgcct tgaggatttg atcgtggtca tcacaccagt tatcgacggt acgggaatca
     5521 atgtgttcgt gcagtttttt ccagtacggg ttatggctac gctgggtcca cattttttca
     5581 acgatagccg ccagggcgga gttctgcgtc gccagggcta cctgaatatg gaactgcaaa
     5641 tcccactcgg aatcacggaa gcattgttcg ccgcgcgcct gctcctggat ggccatcaat
     5701 ttcatgatgt cctgtttcgt tacctgagtt gccgcgaact cggcaatatt actttcgatg
     5761 agttggcgag cctggagtaa ctcaaacgga ccgtaattgg cgaattccat attattgtcc
     5821 gcagcctgct gatggcgcgg ctggttggaa accacgtgaa tacctgaacc tttacgcact
     5881 tcgacatagc cttcaacttc cagcatgatg atggcttcac gaactaccgt acggctgacg
     5941 ttcttttcat cggcaataaa gcgctcagca ggcagtttat cacccaccag atagacgccc
     6001 tgttcgatgc gctctttcag gtcagcggca agttgttgat acaaacggcg tggttcagtg
     6061 atttccatat gcgctccagg catgatacgg cagactatat ttgttatacc acttttgcga
     6121 gtttagctct actcgcagtg atgaaaaagc cgcctgagaa ggcggctttg aagggaaact
     6181 aaaacttaca cgaagcgtta atgttgcggc gcgggatcgt tatgtgtttc ctgcgccacc
     6241 tcaatcgccg gtttgttctg caacacggtc cagataacca gcgcacccag caggtcaaat
     6301 actgccagaa ctgcgaacag cgggctgaag ccgatggtgt cagccagtgc accgacaacc
     6361 agcgcaaaca gcgtacttgc cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc
     6421 acttcgttac gaccgaacac gtcagaagag agcgtaatca gcgcgccaga cagtgcctgg
     6481 tgagcaaaac caccgataca cagcagcata attgcgacat acgggttggt gaacagaccg
     6541 atcatacccg ggccaatcat cagcacagca cccagcgtta cgaccatctt acgggaaacg
     6601 atcaggttca caccaaacca acgctggaac agcggcggca ggtaaccacc gaggatacaa
     6661 ccgaggtcag caaacagcat cggcatccag gcgaacatcg cgatctcttt caggttaaag
     6721 ccgtaaactt taaacatgaa cagcgggatc cacgcgttaa aagtacccca ggccggttct
     6781 gccaggaaac gcggcagcgc gataccccag aactgacggt tacgcaggat ctgaccaacg
     6841 gacattttct tcgccgtgct cacctggtgc tgggcttcct gaccattaat aatatagtcg
     6901 cgttcttcat cggtcagatg cttctggtcg cgcggatgtt tatagaaaat cagccatgcc
     6961 atcgcccaga taaagctcaa tgcaccggag atgataaatg ccatctgcca gctgtgcatc
     7021 acgattgccc ataccaccag cggcggcgca atcatcgcac caatcgaaga acctacgtta
     7081 aagtagccta ctgcgatgga acgctctttc gccgggaacc attcggagct ggctttcaga
     7141 cccgccggga tcatcgctgc ttccgcggca ccgaccgcac cacgagcaac agccaggcca
     7201 ccccagctac ctgccagcgc ggttgcacca cagaacacgg cccacagcac agcaaacatt
     7261 gcataaccga ttttcgtacc cagcacatcc agtacataac ctgctaccgg ttgcatgacc
     7321 gtataagcag cagaataggc tgcgatgata taggaatact gttgggtgga gatgtttaac
     7381 tcttccatca gagttggcgc agctgccgcc acagtgttac gcgtcaggta accaagcacg
     7441 gtgccgagcg tcaccagtgc gatcatatac caacgtaacc ctttaatttt acgcatgtaa
     7501 aacctcatcg ttttgttata tccgcaggaa gcgcggccat catcccgcca gaggctgaag
     7561 atgttagtta cgaccgggta accgaaaaaa aaccggcacg ccccgaacgt tgccatgaaa
     7621 gtttcgtgct aattcggttt ccgtaccggt taacccgatt catctaacaa atgagtgaat
     7681 cggcaatttg cattccgtcg acttatgatt tgcgacggca gaaagataac ttgtcataca
     7741 actttaaaag gtgagagcca tcacaaatgt gggaatattt gtagggacat tacctgacga
     7801 cagcgaggcc agtactggcg cggcctgcag cgagatttac ctctttgaga gtaatttttt
     7861 taactacgtt tattgatcta actcacgaaa atatcttcgg actctggaaa ttggtgtgat
     7921 aactttgtca gcatcgcacc ataagcaagc tagctcactc gttgagagga agacgaaaat
     7981 gactccgttt atgactgaag atttcctgtt agataccgaa tttgcccgcc gtctgtatca
     8041 cgactacgca aaagaccagc cgattttcga ttaccattgc catttgccac cgcagcagat
     8101 tgcggaagac tatcgtttta aaaacctgta tgacatctgg ctgaaaggcg atcactacaa
     8161 atggcgcgct atgcgtacca acggtgtggc cgagcgtctg tgtaccggtg atgcgtctga
     8221 ccgtgaaaaa tttgacgcct gggcggcgac tgttccgcac actatcggca acccgttata
     8281 ccactggacg cacctcgaac tgcgtcgtcc gtttggtatc actggcaaat tgctttctcc
     8341 gtcaactgcc gatgaaatct ggaacgaatg taacgaactg ctggcgcagg ataacttctc
     8401 cgcgcgcggc atcatgcagc agatgaacgt gaaaatggtc ggcaccaccg atgacccgat
     8461 cgactctctg gagcatcacg cagagatcgc caaagacggc tctttcacca tcaaagtgct
     8521 gccgagctgg cgtccggaca aagcctttaa catcgaacag gcgaccttta acgactacat
     8581 ggcgaagctg ggcgaagttt ccgataccga cattcgccgc tttgctgacc tgcaaactgc
     8641 cttgaccaaa cgtctggatc acttcgccgc tcacggctgt aaagtgtctg accatgcgct
     8701 ggatgtggtg atgtttgctg aagcgaacga agcggaactg gacagcattc tggcgcgtcg
     8761 tctggctggc gaaaccctga gcgagcacga agtggcacag ttcaaaactg cggtgctggt
     8821 gttccttggt gccgaatatg cacgtcgcgg ctgggtacag cagtaccaca ttggcgcgct
     8881 gcgtaataac aacctgcgtc agttcaaact gctggggccg gatgtaggct ttgactccat
     8941 caacgaccgt ccgatggcgg aagagctgtc taagctgctg agcaagcaga acgaagaaaa
     9001 cctgctgccg aaaaccattc tctactgcct gaacccgcgc gataacgaag tgctgggcac
     9061 catgatcggt aacttccagg gcgaaggtat gccgggcaaa atgcagttcg gttccggctg
     9121 gtggtttaac gaccagaaag acggtatgga acgtcagatg acccaactgg cgcagctcgg
     9181 tttgctgagc cgctttgtcg gtatgctgac tgacagccgt agcttcctgt catacacccg
     9241 tcacgaatac ttccgccgca ttctgtgcca gatgatcggt cgctgggtgg aagcgggcga
     9301 agcaccggcg gacatcaacc tgctgggcga gatggtgaaa aatatttgct ttaacaatgc
     9361 gcgtgactac ttcgccattg aactgaacta aggtctgggg ttgatatgca atacatcaag
     9421 atccatgcgc tggataacgt cgcggtcgct ttagcagatt tggctgaagg cacagaagtc
     9481 agtgtcgata accagactgt tacgctgcgc caggatgttg ctcgtggaca taaatttgcg
     9541 ttaacggata tcgcaaaagg ggccaacgtc atcaaatatg gcctgccgat tggttatgca
     9601 ttggcggata ttgcggcggg ggaacacgtt cacgcccaca atacgcgcac gaatctgagc
     9661 gatctggatc agtatcgcta tcaacctgat tttcaggatc tgcctgcgca agcggcagat
     9721 cgtgaagtgc agatctatcg tcgcgctaac ggcgatgtcg gggtgcgtaa cgagctgtgg
     9781 atcctgccaa ccgtgggctg tgtcaacggc atcgcgcggc agatccagaa ccgttttctg
     9841 aaagagacca acaacgctga aggtaccgac ggcgtgttcc tcttcagcca cacctacggc
     9901 tgctcacagc tgggcgacga tcacattaat acccgcacca tgctgcaaaa catggtgcgc
     9961 cacccgaacg caggcgcagt gctggtgatt ggcctgggct gtgaaaacaa ccaggttgcc
    10021 gcattccgtg aaacgctggg cgatatcgat cctgaacgcg ttcatttcat gatctgccaa
    10081 cagcaggatg atgagatcga agccgggatc gagcatttgc atcagctgta taacgtgatg
    10141 cgcaacgata aacgcgagcc aggcaaactc agcgaactga agtttggtct ggagtgcggt
    10201 ggttctgacg gtctttctgg tattactgct aacccgatgc tggggcgttt ctctgactac
    10261 gtgattgcta acggcggtac taccgtactg accgaagtgc cggagatgtt tggcgcagag
    10321 cagttgctga tggaccattg ccgcgacgaa gcaacgtttg aaaaactggt cactatggtc
    10381 aacgacttca aacagtactt tattgcccat gaccagccga tctatgaaaa cccatcgccg
    10441 gggaacaaag cgggcggtat caccacgctg gaagacaaat cacttggctg tacccagaaa
    10501 gcgggttcca gcgtcgtggt tgacgtgctg cgttacggcg agcgtctgaa aacgccaggg
    10561 ctgaacttgt taagtgcgcc gggtaacgat gccgtagcga ccagcgccct ggcgggtgcg
    10621 ggctgccata tggtgctgtt cagtactggt cgtggcacgc cgtatggtgg atttgtgccg
    10681 acggtgaaaa tcgccaccaa cagtgaactg gcggcgaaga aaaaacactg gatcgacttt
    10741 gacgcgggtc agctgatcca cggtaaagcg atgccgcagt tgctggaaga atttatcgac
    10801 accatcgttg agtttgccaa cggtaagcaa acctgtaacg agcgtaacga cttccgcgaa
    10861 ctggcgatct tcaaaagcgg cgtaacgcta taaagtcgaa atgagttgtt aataaaaaac
    10921 ggcgtttcat aatgtgaagc gccgtttgct tttccatacc tgctaaggat ttatcatgac
    10981 cgcgtattgg ctggcccagg gcgtgggtgt catcgccttt ctgattggta tcacaacatt
    11041 tttcaatcgt gacgaacgtc gcttcaaaaa gcagctttcg gtctatagcg ccgttattgg
    11101 cgtacatttt tttcttctgg gcacctatcc cgctggtgcc agtgccatcc ttaatgccat
    11161 tcgtacattg attaccttac gcacgcgcag cttatgggta atggcgattt ttattgtgct
    11221 gactggcgga attggcctcg cgaagttcca tcatcctgtc gaactattgc cggttatcgg
    11281 tacgattgtc agtacctggg cgctgttccg ctgtaaaggg ctcaccatgc gctgcgtgat
    11341 gtggttttca acgtgttgct gggtgattca caacttctgg gcggggtcga taggcggcac
    11401 gatgattgag ggtagttttc tgcttatgaa tggcctgaat atcattcgtt tctggcggat
    11461 gcagaaaagg ggaattgatc cgtttaaagt agagaaaacc cctcccgcca tagacgaaag
    11521 gggttaaaca attaattacg cagggcgcta tttgccagac gatcgtcttc tgcctggcaa
    11581 gctgccgcag tgaatagcac gtcagttgaa gagttcagcg cggtttcgca agagtcctgc
    11641 aatacgccga tgataaagcc gacggcaacc acctgcatgg cgatatcgtt cgaaataccg
    11701 aacatattac aggccagtgg gatcagcagc agagaccccc ccgccacgcc ggatgcgcca
    11761 caggcacaca gagaagccac tacgctcaac agcagcgctg tgggcagatc gaccggaata
    11821 cccagcgtat taaccgcagc cagcgtcaac acggtaatgg tgattgctgc acccgccata
    11881 ttgatggtgg cacccagcgg aatagaaacg gaataggtat cgcgatccag attcagcttt
    11941 tcacacagcg ccatattcac cggaatgtta gccgcagagc tgcgggtgaa gaaggcatac
    12001 acgccgcttt cgcgcaggca cagcagcacc agcgggaacg ggttacgacg aattttccac
    12061 cacaccagca atgggttaac caccagcgcc accagtaaca tacagccaac cagcaccacc
    12121 agcagttgcg cgtagcccca cagtgtcgag aaaccggtgg ttgccagtgt tgaagatacc
    12181 agaccaaaaa taccgattgg tgcgaaacga atcaccagtt tcaccataaa ggtaacggca
    12241 ttcgacatat cattgaccag gtttttggtg gtctcgttac cgtgacgcag tgcgaagccg
    12301 aggccgatcg cccacaccag aatcccgatg tagttacctt tcagcaaggc gtcgataggg
    12361 ttggaaacca tgctcattac cagtccgcgc atcacttcga caatgcctga cggcggcgaa
    12421 atatcacccg cgctactgga caagtgcagg gtagaaggga aggcaaaact gaagactact
    12481 gcggccagag cagcagagaa ggtgcccagc agatagagga acaaaatagg gcggatattg
    12541 gttttctgcc cgtgctggtg gttagcgatg gaagccatta ccagcatcaa caccaggatg
    12601 ggggcaacgg ctttcagtgc gccgacgaac aaagtaccta acagaccaac agcttcagcc
    12661 gccggttttg agatccatgc cagaagaatc cccagaacaa ggccgaccag gatttgtttt
    12721 accaggctgc catgagccag acgccggaat agccccggtg aacgttgcgt agtcattttt
    12781 cgatcctttc attgtgttgt cgcacatccc tggtgcgttc tgtcgacgca tctttcagga
    12841 tgtaacaaag tgtttgcaag gtcgagtata aggaaagtta cagcttcggg aagcgaaaaa
    12901 tgctggattt taaagagagg tcatattttg caacttaatg gttacattta tttgacataa
    12961 aaataaagcc caacgtgtgg cttccagact gatggcaacg tcaaaaaaat actcggtcag
    13021 tcccctctcc ctggaaggga gagggagaaa acggtgctca tgttgatgac agggtacacg
    13081 gcttaggcag attaaaaatc atccaacccg ctgtttatca tgccgataat tcacccaggc
    13141 gttgataatg aacgtcatca ccagaatgcc aaacaccacg cccagcgaga cggcgatcgg
    13201 aatatggtag aagtcgacaa tcagcatctt gataccgata aacaccagaa tcaccgccag
    13261 gccatatttg agcatcgaga aacgctctgc tacgcccgcc agcaggaaat acatcgcacg
    13321 caggccgagg atcgcaaaca ggtttgaggt cagcacaata aacgggtcag tggtcacggc
    13381 gaagatagcc ggaatgctat ccacggcgaa aatcacgtcg ctcaattcca ccagaatcaa
    13441 taccagcatc agcggcgtgg catacagcaa gccattctta cgcacaaaga aatgctcatt
    13501 gtcgatggtg tcggtcatac gcaaatgacc gcgtagccag cgcaccagcg gcttgtcgcc
    13561 aatgcctgat tcatcttcat gggcgagcgc catcttcacg ccggtaaaca gcaggaaggc
    13621 accgaagata tacaggatcc agtcgaactg cgaaatcagc cagctgcctg tgaagatcat
    13681 gatggtacgc agaacaatcg ccccgagcac gccatacacc agcacgcgac gttgtaatgc
    13741 cgccggaacg gagaaatagc tgaacaacat cagccagaca aagacgttat cgaccgccag
    13801 cgatttctca atcagataac ccgtgagaaa ggccagtgcc tgtggatcgg caacggcgcg
    13861 accttcggtt tgcaccagat accaccagaa agcggcgtta aacagtaacg acagcgtcac
    13921 ccagaccagc gaccaggccg cagcctgttt catggtcatg gcatgtgccc cacgacgccc
    13981 ctgcaacaac aggtcgatag ccagcataat ggcgacaaca acagcgaatc cgccccatag
    14041 caacggcgtg ccgacagtat tcatagaagt tccttacaca taaaaaaaac ggccaacgtc
    14101 ggaagacgtc agccgctgct ttttatgcat agacctcgcc ttccggcaag gtctcactta
    14161 caacaaaaaa ggaatacgtc tgcgtattcc tttcgggttg cccggtgacc ggatgtgatt
    14221 tttcacacat cgtaatgacg atcgaccggc aatgaagtta ctcccctttg caggtaacaa
    14281 agtatgacag accattcacc cagtcaatgg tctgttacat gccttttaca ctgctttacg
    14341 gtaacaatta ttgttttacg ctatctgccg gaaaaatcac ccctgtctgg cggcggatct
    14401 ctgtcagcaa tttggcggtg atgcgactga ccgccagtcc cggatgatcc accagatgct
    14461 catccaccag ggtagcgaac agctctgctt cgtagagcat ggtattaata tgctgcggct
    14521 gggtgagatc ctgcatctgg ctgccacgcg gcacgaagca cactttctgg cattcagaca
    14581 gtttttcaat caccagcgat cccgcttcgc cctgaatctc gcttgccagg acagaatcac
    14641 tgacctttga gtgctgcaag gtgacgctga aatcaccgta atccatcacc accacgccat
    14701 gggcatctac accacttgcc agtaaactgg cggttgcctg cacgcttttc ggctcaccaa
    14761 ataacgctac cgccgacgcc aggcagtaaa agccgatatc cataattgaa ccgttagaga
    14821 atgccggatt aaaggtgttg ggattctcac catccaggta acgttgatac cgcgaggaat
    14881 actggcaata gttgaaaaag actttccgca gtttgcctac tttcggcagc gcctggcgca
    14941 gcaaatgaaa gttcggcagg ctggctgttt taaatgcctc aaacagcacc acctgattat
    15001 cccgcgcaca ggcaatggcg gcatccactt ccgccagatt cgacgccagc ggtttctcgc
    15061 aaatcacatg aattttgtga ctaaggaaaa gttgtgcctg ggaaaagtgc agggaattcg
    15121 ggctggcaat atacaccgcg tcaatggcat cgctttccgc catcgcttcc agcgaggtaa
    15181 acagatgctc gacagaaaaa tcattggcga aatgctgggc ctgttcaagg ctgcgggaat
    15241 aaacggcggt taacttgtat ttaccgctct catgggcggc ctcgacgaac tggcgagtga
    15301 tccagttcgt accaatcaca gcgaaacgta tcataacgcg tggatactcc ataaagggat
    15361 tctgccgtca atgtagcacg cctttttcat cttcgacgtg cgcagatacg cattattctt
    15421 tcgccggaca gccatgctca ctaaacggcc caatcataac cgacggcccg agatatttcg
    15481 gctggcgatg gaggattttt gcgtcgatca ccgcgctgac gcgagcgagt ttctcccgta
    15541 actgagtata aaaaccgtgc ttcatattca ggtctggtgc gttaaacccg atagcatcga
    15601 tgccgtattg ttttgccagc cagatggcgc gttgattatg aaattcctgc gagataatgg
    15661 tgatatggtt ttccccaaac acctttttgg cacgcactac cgaatccagc gttgagaatc
    15721 cggcatagtc gcagaagatc actttcgccg gtacgccttt agcgatcaaa gcctgctgca
    15781 tacctgatgc ttcatcgtaa tttttacgtc cgttatcgcc actgactaac agccatttca
    15841 cttttccggc gtggtacaac tctgctgcgg tatcaatacg tcgggtaaag tagcgattac
    15901 ccggcctggc ccccagcaat aagcccacgt tacgcgccgg aacgacgttg acgtcgctcc
    15961 aggtcagctg tttgctggcg ttgatcatta cgcgatcggc aataaaaagg gtcgctcctg
    16021 ccacaagtaa cagtaagcag gcaatcttta gagtccggcg agaaaaacaa atacggagaa
    16081 gaaggcgggc aaatgcgcgc agcatggtcg ttatccttaa cgaaaggggg gaggtcgatt
    16141 ttaggcacta aagaaagaaa agataacgcc tgacatcatt ttcagatttt gtcctgccca
    16201 agcgacaact gggttagcca cagtcgggtg tcgaactcaa gctggtggta ctggggttcc
    16261 atatgacagc acaactggta aaacgctttg ttatgctctt tctccttcag gtgcgccagc
    16321 tcgtgcacca cgatcatgcg caaaaacggt tccggcgcgt tgcgaaacac ggtagcgacg
    16381 cggatctctg cttttgcttt cagcttgccg ccctgcacac gcgaaacagc ggtatgtaac
    16441 ccgagcgcgt ttttcagcac atggatcttg ttgtcataca tcactttatt gatcggcggg
    16501 gcattgcgca gaaactgatt tttcagatcc tgagtatatt gccagagggc tttatcggtg
    16561 gcgtagtcgt gtgttcccgg atagcgtttt gccagcacat cgcccagacg ctgttcgtta
    16621 atcagtgttc gcacctggga aagtagctgc tcgggataac cctgaagata agtaagattg
    16681 ctcataaccg cccacgaaaa agagaaaaag ggtatactca cgcacccttt tcaggggcaa
    16741 tgtcgaaatt ttatcattca ggagggccga tgagccactt agacaacggt ttccgttcac
    16801 tgacactaca acgttttccg gcgacggatg acgttaaccc gctacaggcg tgggaagcgg
    16861 cggatgaata tttgctgcaa cagttggacg acacagaaat ccgcggcccg gtgttgatcc
    16921 tgaatgatgc ctttggtgcg ttaagctgcg cgctggcgga acataagccg tacagcattg
    16981 gcgactcata catcagtgaa ctggcgacgc gcgagaattt acgcctcaac gggattgatg
    17041 agtcgagcgt gaagtttctc gacagcaccg ccgactaccc gcaacagccg ggtgtggtgc
    17101 tgatcaaagt gccgaaaaca ctggcattgc tggaacagca actgcgtgcg ctgcgcaaag
    17161 tggtcacgtc ggatacacgt attattgccg gtgccaaggc ccgtgacatt cacacttcca
    17221 cgctggaact gttcgaaaaa gtgctcggcc cgaccaccac cacgctggca tggaaaaaag
    17281 cgcgcctgat taattgcact ttcaatgaac cgccgctggc cgatgcgccg cagaccgtta
    17341 gctggaagct ggaaggtact gactggacta tccacaacca tgcgaatgtc ttctcccgca
    17401 ccgggcttga tatcggcgcg cgcttcttta tgcaacatct gccagagaat ctcgaagggg
    17461 agattgtcga tctcggttgc ggtaatggcg ttattggtct gacgctgctt gataaaaacc
    17521 cgcaggcgaa agtggtgttt gtcgatgaat cgccgatggc ggttgcttcc agccgtttga
    17581 acgttgaaac caacatgcca gaggcgttgg atcgctgcga atttatgatc aacaacgcgc
    17641 tctccggcgt ggagcctttc cgctttaatg ctgtgctctg caacccgccg tttcatcagc
    17701 aacatgcgct gaccgataac gtcgcctggg agatgttcca ccacgcccgc cgctgcctga
    17761 aaatcaacgg cgagctgtat atcgttgcca accgtcacct ggattacttc cataaactga
    17821 agaagatttt cggcaactgc accactattg cgacgaataa taaatttgtg gtgctgaaag
    17881 cggtgaagct cgggcgtcgt cgataaggtt gagaacaacc tgccggatgc ggcgtgaacg
    17941 ccttatccag cctacgttca gaacgttacc tgtaggccga ataagatgcg ttgcatccgg
    18001 cgcttcaaat aatgatttaa atctccagcg ccagccgtgt gccttgggca attgcccgtc
    18061 gtgcgtccag ctccatagct acatcgcagc cgccaattaa atgcaccgtt ttcccgctat
    18121 caatcagcgg ttgcgccaat gcgcggtttg gctcttgccc tgcgcagatc accacattgt
    18181 ccactgctaa tacctgcggt tcgccgttga tcaccacatg cagcccgtca tcgtcgatct
    18241 tctgataact tacacttggg atcattttca cgccgcgcga gagcagggtg gtgcgatgga
    18301 tccagccggt ggttttgcct aacccctgtc ccggtttgct ggctttgcgc tggagcatca
    18361 caatctgccg tgggctacgc gggatctgca ttccctgcgg gcttaagcca ccagcctgtt
    18421 gtaggctgct gtcgatcccc cattcattac agaacccggc gatattctgg ctggtggatt
    18481 cgcccggctg acttaaatac atcgccgtat caaaaccaat cccgccacaa ccgatgatgg
    18541 caactttgtt gcccaccggc gctttgtcgc gcagtacatc gagataactc aataccttcg
    18601 gatgatcgat cccgtcgatg ggcggagtgc gcggcacgat cccactggcg aggatcgttt
    18661 catcgaacgc ctgtaactga tccgccgtca cggtgtgatt gagttttagc gtcacgcccg
    18721 ttacttcgat catccgccgg tagtagcgca gcgtttcgta aaactcctct ttgccgggga
    18781 tctgtttggc gatattaaac tgcccgccga tctcactgtg cgcgtcaaac aacgttacct
    18841 gatgaccacg cgccgccgcg ttaatggcaa acgccagccc agcaggtccc gcaccgacca
    18901 ccgccagatt ttttttctgc acggcgggaa ggattagcat tttggtttcg tggcaggcgc
    18961 gaggattcac caggcacgag gtgaccttgc caacgaagat ctgatcgaga cacgcctggt
    19021 tgcagccaat gcaggtgttg atctcatcgg ctcgccctga ttgcgctttt gacagcagct
    19081 ccgcatccgc aagaaacggt cgcgccatcg ataccatatc ggcatcgccg cgcgagagaa
    19141 tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt caccagcggc agcgagacgt
    19201 gacctttcag tttgcgcgtg acccagctaa atgcgccgcg tggcacgggc gtggcgatgg
    19261 tcggaatacg cgcttcatgc cagccaatgc cggtgttgat aatggtcgcg cccgccgctt
    19321 caatggcctg cgccagctct accgtttcgg caaaagtccc gccgtcttcg accaggtcga
    19381 gcatcgacag tcggtagata ataatgaagt cgttgccaac gcgttcgcgt accgcactca
    19441 ccacttcaac ggcaaatcgc atccggttgt ggtaatcgcc gccccactgg tcactacgct
    19501 gattggtgcg cagcgtcaga aattcgttga tcaaataccc ttcggaaccc attacctcta
    19561 caccgtcgta tcctgcctcc cgtgccagtt gcgcgcagcg ggcgaaatcg tcgatcagtt
    19621 gcaggatctc ttcatgggtg agttcatgag gaacgaaacg gttgatgggg gcctgcaatg
    19681 cggacggtgc gaccagatgc ggttggtagc tgtagcgccc ggtatgcaaa atttgcaggg
    19741 ctattttgcc gccttcctga tgtacggctt cggtaatggt gcgatggtgt gggatctggc
    19801 tggcgtcatt gagcattgcg ccgccttcca tgccaacgcc tgttaaatct ggtgcaatgc
    19861 cgccgctaac aatcagcgcc acgccgtgac gggcgcgttc ggcataaaac gcagccagcc
    19921 gttcagcgcc gtccgggtat tcctccagcc cggtgtgcat tgaacccatc aacacgcggt
    19981 tttttaacgt ggtaaaacct aaatccagcg gggcgaacag cgacgggtag ctcataaaat
    20041 tgtccagtat gtaaaataat tgttatgtgg tcggatgagt tctaatttag ccgtcgccgg
    20101 agaaaaggga aaaggggagt gcggggtttg tgatgggatt caaaaagaat acgcccggtg
    20161 gtaacgccgg atgcgacgct tgccgggccc tatccgacct gggggattgc acatgtaggg
    20221 cggataaggc gttcacgcag catccgccag tggcgcggtg cagacgccgg atgcgacgct
    20281 tgtcgggtct tatccagcct acgggaacga acatgtaggg cggataaggc gttcacgccg
    20341 catccgccag tggcgaggtg cagatgccgg atgcgacgct tgtcgcgtct tatccagcct
    20401 acgggaactg ggggattgca catgtagggc ggataaggcg ttcacgccgc atccgccagc
    20461 cgttgcaaat tattgcttac ggaaaaaatc gttatacaac atatacaaat gtgcggcact
    20521 ccaggagaaa tttggtgcgc cttgctgcgc gccagtcagc gggttgtaat tctcctgaat
    20581 tgggccatcg gcggttaatc ctttggcatg ccggaagaac gtatccgcca gcttcagggc
    20641 atcatcacga taaccgtaac gctccatccc tttcagacca aaccagaact gatccaccca
    20701 tacgcgcccg cgccagtaga tatccgcgcc aaaggccggg ttggttaacg ctgccgttcc
    20761 cagcgggaca aaggtgttga actctttagg atcgagcatc accttcacca ccgcgtcggc
    20821 gttggcctgc gttgccgcac cgttaaacag cggcgaccag ccttccggcc ctttaccgcg
    20881 ctcaacaatc ggtttgcccg cgcagccgtt cgccagcggt ttatcttcaa tacgcacgtc
    20941 atagaagtac tgcgtagccg ggtcgaacat acaggtgttg atgtagtccg cgagctgctg
    21001 tgccaactgg cgatagcgct tagcctcttc cggtttaccg agaatcgtcg ccatctccgc
    21061 cagataatgg ttatcgctgt acatatagct ggcctgatcc accgactcct gcaatagcga
    21121 gtaccccagc aacgttccgt cctgactgcg gttttcggcg aatttcaccg tccagtcgct
    21181 acgtttgccg ccattagcga catatttatc cagctgttct ttgtcgataa aaccaaagac
    21241 ggcggcgtca tcacggcccg attcccacga tgcagcaacc tgtgccggaa tttccagact
    21301 gtcgtactgg cctttctcca ccacgcgggc gtagttgttc agcccagact gcgtctcttc
    21361 tttatcgcct tttttcaccg taaacagcat ctcgccgctc tcagtgttgt gggctttgtc
    21421 gcgggtcgcg ccatattccg gcacgccgtt gccgttgtga tcgcggttac gtaaccacca
    21481 gtcgtgatag gccaccagtt tcgggtacat ctctgccagc caggttttat cctgggtgac
    21541 gttatacact tccatcaccg accaggcggc aaggctgggt ttggtattac gttcgttcca
    21601 gttgccgcca tcgccaccac gctcggggct aaggttccac gctatcaggt cggggacaaa
    21661 gcccacatcc tgcggacgca cgcggtcgcc gggctggatc tgccaggaga agaccgcgcg
    21721 gatattctct ttggcgatgt ccggattgaa atgcgccatc gcaaacgcct gcttccaggt
    21781 atcccacggc caggtctgat tgccggagaa ccagcgcccg gtcaccgacg gcgtgacggt
    21841 gttgtatttc aacgcgccgc caggcgagcg ccagttaccg ttgagcgttt cgatcgcttt
    21901 caccgcgacg cgcgtctgtt ccggcgtcgc atccggattg gttaaccctt tcttcagata
    21961 ctcttcccag cgttgctgcg aggcggtgag ataaaacgcc ggacgcgcca gaatatcgcg
    22021 gatctgtatt tgctctttgc taacttcctg agcggtcagc aggtgggagt aggtggtata
    22081 gagcgtcgtc gacccgttga tatgcgcctt actggtaaag cgattaccgt tgatttcagt
    22141 ctgtaccggc agggatttat gcacctgata ttccgattcg ccggaggtca gcagatccca
    22201 ggtggcgcgc actttgccaa aggtgacttt caggccatca cgggtggcgc tgattttgcg
    22261 ctggtagtca gggtattcgc cagcaatggt tttatcggaa agcggtttcc cttctttcgc
    22321 ttccagtttt tccagcagtt cgccatccca caccagatcc agcggtttat tgctgatgat
    22381 tttagtttcc agtagtgacg tgcgcggcgt ggcgaagcgc agagtcattt cgacctgcac
    22441 atcttttgct gtcagttttt gcaccagcgc gccgggaata ctgtatgcct ccagcgtgaa
    22501 gtcgactttc ttgccgtcct gccagacggt caggcggtcg aaattgctgg ccataaagtt
    22561 gatgtactct tccgtcagca gcgcaacgcc tggaaaaccg cccatggtgt tagggccgtc
    22621 tggcaacaga tgaccatgcc aggctccgag atcgaaaaac ggattaaaac gctggtgatc
    22681 gtcgtaatcg taatccttca tgtactgcgg cgcaccagta cggttaatca cgtttttata
    22741 attgtcggcg ttagcggcat gggcggaaaa acttatcagc agagcgcagg ttactggcgt
    22801 taaaatagtt ttgattttca tgaaattaca gtccttatta ccagtagaaa tattgcatca
    22861 ggaagatttg gtcgctggga tcgttagagg catttttcat gtagaagcgg ctatcaaaag
    22921 ccgttgcgaa acgtccgccg ccatattcgt accagatacc aaactgcatg aaagaggtgg
    22981 tttcatcgtc agcgtggtca gggcgatgtt tggcgtaggt gtaagcggtg gagagataaa
    23041 cgccctttaa cgcttcatac atggcgctca ccatcatccc gctttcactt cccggtacgt
    23101 ccaacccgtc ggcacgtccg gtatggtgcc aggcgcgagc ggcaaagtta ggggtaagca
    23161 ggcccgtgag atagagttgt cctgtgccgt cggtaatttc cagaccattc atccaggtga
    23221 tgccttcggc aaggtcatac tggatgtaac cgttcatcat tgccggatag gtgtatttgt
    23281 cgtcgtagcg atcgtacttg ccaaaatgta gccatgcttt gctttcgtca tgatgtccgg
    23341 caggtgtggc ggtgacgcta taacgaaagt tgccgcttaa gttctgcacc ttcagcgcgg
    23401 tcataatgtc gcgggtattg ggaatgacgt agcccagatc gggtgtgaag tcgccccacc
    23461 attgcaggtc atccagcgag gaatcctgac gcagactgag catgatttcg gtgccatcat
    23521 tggttcgata accgccatag aaacggttaa tcccaccttc gaaaccgccc cagctatggt
    23581 caggcaccca ggcattgccg tcgtggtcgg cctgcacggt ccagccttcg ccgtaaatca
    23641 ggccgaacca ggagccgtac ttcatctcca ggccaccttc aatataggtg ccgtcgctgt
    23701 atttatgttt attctcaccc tctaaatcca tatatccacc aacccctaat tcgccgtaaa
    23761 aacgaaatga aggagaagat gtcgaggtaa ttgcgggtgt ttttgtgtcg tcatgttctg
    23821 ccggacgtgc ttcttctcct gccgcagaaa aggcgtagca aaacggcaga gcaagtaatg
    23881 ctctgcatgg tgcagtgatt aatttcattg tgttaatcca tgagctaaag aaaaagtaca
    23941 gccttttatc gttgtaatta taaaaatata aaggcagaaa ctattttccc tctcctcatc
    24001 caggaagagg gaaaatgcct gctcattttt tattagtctg tttaatcaac attgctggct
    24061 ggcgtcgctt caatatggcg ctcttcagcc gttaatccct ttatatattt actgtatttc
    24121 caccacgcaa agccaaggaa gataacaata ccgccgacgt tataaaaaat gatggtcaga
    24181 atattcgcgc cagtcgggaa tgtcgaagcg acaaacccta cggcaaatat cgcaatcagc
    24241 attgaaacaa cgataatacc ggtgcggcgg gagcccatac ggaaatcgcg tggcaggtga
    24301 tctaatttgg cgcgtaaatt caggtaagcc agcatgatga ataacggcgg aagcatggac
    24361 gctgcggcgg tcatattaat aatggtattc atcagatcct gcacggtatt ggaaccgagc
    24421 atcgggataa tcatcagcgg gatgacgatc aggaactgga tccacgctgc gcgcgccgga
    24481 acgccgtttt cgttcagttc tacggttttc ttaccaaaga tgccttccgg gatttcggag
    24541 aagaaaattt tcaccggcgt tgcggtccac atcagcaggg aaccgaacat tgcggtaaag
    24601 gacaccagac cgacaaagcg attcatcaac gcttccggca gaccaaaata cgccgccatg
    24661 ccgtggaata cctgcaccga tccgccagta aatttcaact ctttgctgct gacgaagacg
    24721 ttaatcagca ccgaggagac ggaatatagt acgccgataa aaatcccggc gaggatgatc
    24781 actttaacga acgatttcga accgcctttg acgtcgttaa cgtacacagc gacggactcc
    24841 gcgccacctg cggccataaa aatccaggtg gtaacgccga ggaacgccca gttgaagttc
    24901 gggatcatcg cgtcaacggt gatggggtca gcaggctgta cgccgccaac cagcgccgta
    24961 cctgccagta aaatgtagga gagcgttaac agcagcatca gcgttgaagt gacggaggta
    25021 attggcccca gcattttcgc cccgttggtg gaaacccagg tggagaaggc gaacagcacc
    25081 atactgataa tggtggtggc aaccggcgtc ataatatatt cgtagccgag gaaggcatac
    25141 gaagcataag caataacgcg cggcaacagc gaggtgaaaa agaacagatt tacgaaccag
    25201 taggtatagg cagtaataaa tgcccaacgt ccgcccagcg aactttttac ccacgcgtag
    25261 acaccggctt ctgagttttt atttaacgaa acaaattctg cgatgatcag acagaaggga
    25321 ataaaataaa aaatcgtcgc gaggaaaaac atcggtgccg aggccagtcc aagctcaata
    25381 ttattattga taacgttgtt aaagctgtaa acggcggcaa aagtcagcga gagcaagccg
    25441 aatttgccga ttgtattacg tttggtatca gacataacac atctccgaaa atgagtaggg
    25501 tagcggtggc ggcaaactcc ttttgccgcc gtagttgtta tttgttatgg aaataaccat
    25561 cttcgatggt gactttgaga accacttttt tgaccgcgtt attgcagata aaccgatatg
    25621 cttcatggat atcgcaaata acgatttgcc cttcgtggac ctctacggtt tctccgcagc
    25681 cttttaaata ttcgcggtca gtttcatcac gataatattc cactacctgt aatgtctctt
    25741 tcggcgcata ttcaattttt tgctgccctt gcaggtagta atgcacttca aaatagcgtc
    25801 gatgcccggt aaatagcgca tcggtcgcgg agtctgtctc cacgcggtaa gtcaatgagt
    25861 caccgatgga gtgggcgacg ccaggctgaa tgttgtcgat attttcaatc gcttcaacgc
    25921 agcgttgcca cttcttgcca gaggcgtaaa tctggcggaa ctgttctaag ttatcgatga
    25981 tcctcattgc ttattctcgc tgtgcaaatt cgtggaaaag aaccctgcgc cgaacttatg
    26041 cgacgccagg ctttgcgcgg tggcttctcc gccagaaacc ggcagcaacg taaagccgta
    26101 gctgaagtca cggaaccaga cgcgccagga gtccagcacc tcgctgcccc aggagttgga
    26161 gccgaggcca agcagctggt ggtcgagatt cagtgtgatg tcatcgctgc gctgtagctc
    26221 gttgcagtgc tgggcagcgt ggatgttttc ctgggtatag tgccaggcgc tgaagttaat
    26281 tgggcgctgc ggaaccacca gcagaccgtt gccgtggcgg ttagtcagtg ccgtccagcg
    26341 gacatgctga cggttaccgt tgttctgcgg gaagggatag ttctcgaaca tggcatcgac
    26401 ggtgctgcgc cagatatcga tgatgttagc ctgctggctg tcggcgtagt tttcgcccgg
    26461 tccacgaccg taatacgcca cctgatcgta ttcgccgtta atccccatgg tgaaaccgat
    26521 gcacggaatg atgtgcggat agtcgccgta acgcttgccg gaaagcgcca cgttaacctg
    26581 gccatcggca gcgatgcgcc agatgtaggt gcagcgcatc ccgaagtcaa acaccggcgg
    26641 tgctataacc gtgcggctga tgatcaacac ttcaccatcg ctctgttcta cggcaaagtc
    26701 gcgcagatgt tcctgcatga tctgcaaatg attcggttgc cacagccctt cgtactcctg
    26761 cttgtggttg tcgatcattg gcttgaagaa gttgatcttt ggctcgcggg tcagcagcga
    26821 ctcgccattc acctgccagg atgtcggttt gccactcatt tttgagaagg tgatcgcgaa
    26881 gttgtagccg cgaacggtgc agctcaaacg atcgtcttcc agcgtcagcg gacgcgcatt
    26941 atttggtgcg aaaggcactg gctgcgcggt gttttccttc agcgggaact gataagtggc
    27001 gatagaatgc ccggcttcgc tgtagcgggt gcgggaatct ttggtcaccg taatgttgag
    27061 gaacgcttcg cgggcgtcca gctgcggcag cgtgatctgc aagggggctt cgctgttcgg
    27121 cgcaacgtcg cgcagtttaa tctgctgcgt cgcgagcgtt tcaccttcgg cgcgcacctc
    27181 tgcgtgcagg gtgtagtcat ccagcgtggt aaaccacagt ttattttcga ctttcaactc
    27241 gccgcgagtc agatccaacg cgtggatttt taccggcgcg ataacctgtt tgtactcttt
    27301 caggcccggc ccaggcgtct gatcggaata gatcaaacca tcaagacaga agttatagtt
    27361 gttgggatag tcgccgtagt cgccgccgaa tttataccag acattgccgt tgtcatcctg
    27421 cgcctggatc ccgtggtcgc accattccca gacataatgt ccctgaatgc aatcgtgctt
    27481 atagaagacg ttctggtact ccgtcagccc gcccggcccg ttccccatcg catgagcata
    27541 ttcacagatg atgcgcggct tcggatgcgg gtattcacca aactcattca tcagcggcac
    27601 gcgggtgtac atggtggaaa taatatcgac cacttcagca tcgcgatctt cttcgtaatg
    27661 caccagtcgc gtgtcatcca gcgctttcgc tgcgtggtac atcgcgcgga tgttacagcc
    27721 atagccggat tcattgccca gcgaccagat gatgatcgac ggatggtttt tctgcgcgtg
    27781 aatatggcga acaatgcgct cgacgtagac tttttcccac tgcggatcgt cggtaatacg
    27841 gctgatatcg ccgacattag caaagccgtg cgattcgacg tcggtttccg ccatcacaaa
    27901 caagccgtag atatcacaca gttcgtaaaa acgcggatcg ttcgggtagt gagcggtacg
    27961 cacggagttg atgttgtgct gcttcatcaa ctggagatct ttctcgacgc gatccattcc
    28021 aacggcgcgg cctttgcgat gatcgttgtc gtgacggttg acgccgtgca gcatcacata
    28081 acggttattg atccagaaca gaccgtcgcg cactttgata tcacggaagc caacgcgttg
    28141 tggtaccact tccagaacgt tgccgttggc gtctttcagc gtcatgacca gatggtaaag
    28201 ataaggggat tctgctgacc attgctgggg ctgttcgaca gtaaaagcaa agctggcgct
    28261 ggtcagtttt tcaattgcca aatgatcaat ggtgctgctg tgcaccacgc gttcgccatc
    28321 aaacagggta tattccagcg tcgtgacgac aggggaggct gcgagatttt ccagcaccac
    28381 ttcgcaggaa agcgtggcat cgcaataggc ttcgtcaaag tcggtacgca cggtgaaatc
    28441 gttaatatgc gttagttgtt ttccgatcag ataaacatcg cggaagatcc ccgccgacca
    28501 ccacatatcc tggtcttcca cgtaggtaga gtccgcccac tgcatcacgc gcacacacaa
    28561 caggttgtcg ccggttttaa ccatcgcgct gatatcaaac tctgcggtca ggcgactgcc
    28621 cttgctgaaa cccacatact gaccgttaac atagacttca aaataggttt cgacgccgtc
    28681 aaatttaatc agcgtctgtt taccctgcca gccgtcactg agggtgaaaa tacgttgata
    28741 ggcaccggtt gggttatcgc tggggacaaa cggcacatcg atggggaacg gaaaaccttc
    28801 gtcggtatat tgcagtttgc cgtgaccttc catttgccac atggcgggga cggtaatatg
    28861 cccccagtca gccattaact cagaggtgaa ggcttctggc acttgcagcg gatggtcaaa
    28921 aaagtggaaa ttccactgac cgcttaaggg cagaaacagg ctgctcgttt cgcgggcaaa
    28981 ggtacgcgcc tgcgcaacag aatcatatga aaaaaagtac gcacgcggcg caagtcggtt
    29041 ttcgtgggtg agctgaatgt tttcccagcg attcataggg cctcccggta gagagaaatc
    29101 ggaaccattc atggcgatgg tagtttgtgt aattgctggg gagtttagta aaatttttac
    29161 taaaaagtag cgggtaaatg catttacttt aactggatca cgaaaataaa cggcagcact
    29221 gcccggcaaa aacgattgcc ggacagtgtg taaggggatt taacgggtcg tgccgcgcag
    29281 ttttaatttg ctgggaacga agactaacag cggcagcgcg cgaccatcgc gggctttttc
    29341 atacaccagg tttacgccct gacttcccat catttcggaa tggatgcgca cggtggagag
    29401 cggcggaaag gtaaatcgcg cggtggggat atcgttaacg ctgataagcg aaatatcctg
    29461 tgggatgttc aggccacgtt catgaattgc ccgcagtacg ccgatagcaa tggaatcgga
    29521 agcaacaaac agtgccttcg gatagtcttc ccgcgccagc atctgttttg ccagttcata
    29581 acccgacgaa ctggaaaaac cgccgcgcca gatatcctct tcgcgtacca cttgtttcag
    29641 acggccatat tccgcaaagg cgacctcacg aatatccgcc ttgccaggct catcttcacc
    29701 gccaataaaa ccaatgcgat taacgccctg gttgatatag aagtcgatga tttctttact
    29761 gatgcgtgcc agatcgatat ccaccgcatc gtaaccgctg ccgggttcgt gaaagtcgat
    29821 aaaacagata ttgtcggtca acgcacaggc ggcggcgcgc agggcgggtg tgggtttgcc
    29881 gacaattaaa ataccggtga cgtttttaat gtctggtaat ccgttgtgtt cataacagtt
    29941 ggtcagttcg atcgccagct tttcgcactg agtttcaatg ccgtggcgga tcgccagata
    30001 gtaaggatcg ttgatctcca gctcctgctg gtagctgtag atagccagaa tatggtgttg
    30061 gttgactgcg cctgtctgta gtttacgggc gctactggtc ttgtactcca gcttttcggc
    30121 gatctcgaga atgcgatgtt tcgtctcttc tttcacattc aatgtcggat cgtcatttaa
    30181 gaccctggat actgtcgcca gggatacgcc agcttcgatt gcgatgtctt ttagtgttgc
    30241 catgtttacc ttttttactt tccctgatgt gcgcttccat tgtagaggaa gtgcttatcg
    30301 ggtcacctgt tttcagtaaa taaagcgcgt ttatttgacg ctgtaacggt aacacagcgg
    30361 ctcacgaatg tgatcacgcc tgcattctcc atttttagcc tacaggattg agaaaaactt
    30421 ttgctatagt ttagtaaaat tttactcgaa gggaggaagc aatggaaacc gtggcttacg
    30481 ctgattttgc acgtctggaa atgcgcgtcg gaaagattgt ggaagtgaaa cgccatgaaa
    30541 acgccgacaa gctgtacatc gtacaggttg atgtggggga aaaaacgctg caaaccgtga
    30601 ccagcctggt gccgtactac agcgaagaag agctgatggg aaaaacggtg gtggtcttat
    30661 gcaatctgca aaaagcgaaa atgcgcggtg aaacgtcgga atgcatgctg ttgtgtgcgg
    30721 aaaccgatga cggcagtgaa agcgtgctgt taacgccgga gcggatgatg cctgcgggcg
    30781 tacgtatcgt ataaaaagat cggatggcga cgtcgtatcg ccatccgatt tgatattacg
    30841 cttcttcgac acttactcgc atggccgcca gcgccttacg cgccgctttg atcaccagtt
    30901 cacactgttc aatggtcagt gtcagtggcg gttcaatgcg gatcgttttg gcgttattga
    30961 gcgttccggc caccagtacg cgctggcgga acatctcgct ggcaaagtta tagccgattt
    31021 cgttatcaac aaactcaatc gccatcaaca tccctttacc acgtgcttcc tgcaccagat
    31081 cgggatattc ccgcgccagt tgacggaaac cgtccagcaa catatcgcct ttttgttcag
    31141 cctgagccgg taagttttgt tccagcaaca cattgatggt cgccagcgcc gccgcacagg
    31201 ccagcgggtt gccgccaaag gtggtggtat gcaggaacgg gttgtcgaac agaactgaga
    31261 acacctcttc agtggcgatg gttgcgccaa tcggcatcac accgccgccg agggcttttg
    31321 ccagacacag aatgtccggc tgaacgtttt catgttcgca ggcaaacatc ttgcccgtgc
    31381 gccccatgcc cgtttgtact tcatcgagga tcatcagtgc gccgaactca tcgcacagct
    31441 tacgcacggc ggtgagatag cccggcggcg gcagaattac gccaccttca ccctgaatcg
    31501 gttcgaggat caccgcagcc acatcatcac cggttttttt gcactcgtta agagccgtgc
    31561 gcatggcttc gatattgcca aacggcacat gacggaaccc tggcagcagt ggcataaacg
    31621 gtttgcggaa ggtcgatttt gccgtggccg acagcgcgcc aagtgattta ccgtggaacg
    31681 cgccgctggt ggcaataaaa gtaaatttgc cgcgcggcga ctgataagcc ttcgccagct
    31741 tcagcgccgc ttcaacggac tcggtgccgc tattacagaa gaagctgtat ttcagtttac
    31801 cgggcgttag cgcagcaagg gttttcgcca gcatagcccg taacggatcg agtagctcct
    31861 ggctgtgcag tggttgtttc gcaagttgat tctgtacggc ggaaaccaca actggattac
    31921 ggtgccccac gttgaaaatt ccaaaacctc ccaggcagtc gataaactcc tgtccctggg
    31981 tgtcgacaag cgtatttaaa cctcccgctt gccactctac ggctccgtaa tccccgccgg
    32041 cggtaacaga tttgcgatac tctaaaaacc ccggattgac atgctctttg aagtattcaa
    32101 tcacctctcg gttaagtgct ttcatctcct catgatccag cgttcgcttt tcaatgagat
    32161 tcagggcgtg ggcgctgcac gctaaagccg atgcgctcga aggtaacctg ttcaaaatat
    32221 gctccggagg ctcgcgtatc acatgatacc gatttaagta ttgcagggat tgcgccactc
    32281 cggctgcgat cgcgaaaatc gggataaaca caaatgtggt tatcatttgc gcaatattta
    32341 atgctatttg ttaacatccg ttgttatttt gcacggcttt tgtcgggtat ggatttgata
    32401 attgctgcgc aaatgcacgt tttcagggca ataattgcct gatttttggg caagataatt
    32461 aacgttttcg tatagtaaca gagtgaaagt attttaaatc aacgaattag atttgtgctt
    32521 taatcgcaaa ttgcgatcta aatcaaatta atcggttaaa gataaccgca gcggggccga
    32581 cataaactct gacaagaagt taacaaccat ataacctgca caggacgcga acatgtcttc
    32641 tcatccgtat gtcacccagc aaaatacccc gctggcggac gataccactc tgatgtccac
    32701 taccgatctg caaagctata tcactcatgc taatgacact tttgtgcagg tgagcggcta
    32761 taccttgcaa gagttacaag ggcagccgca caacatggtg cgtcacccgg atatgccaaa
    32821 agcggcgttt gcggatatgt ggttcaccct gaaaaaaggg gagccctgga gcggcatcgt
    32881 gaaaaatcgc cgcaaaaatg gtgaccatta ttgggtgcgg gccaatgcgg taccgatggt
    32941 gcgcgaggga aaaatcagtg gctatatgtc gattcgtacc cgggcgacgg atgaagagat
    33001 cgcggcggtg gagccgctgt acaaagcgct gaacgccgga cgtaccggta agcgtattca
    33061 taaaggcctg gtggtgcgta aaggctggct gggtaaactg ccttcattac cgcttcgctg
    33121 gcgggcgcgt ggagtgatga ccctgatgtt tatcttgctg gcggccatgc tttggtttgt
    33181 tgctgccccg gtggtgacgt atatcctctg tgcgttagtg gtattgttgg caagcgcctg
    33241 ttttgaatgg cagattgtcc gcccgataga aaatgttgcc cgtcaggcac tgaaagtggc
    33301 gaccggggaa cgtaatagtg ttgagcatct gaatcgcagc gatgagctgg ggctgacatt
    33361 acgtgcggta gggcaacttg gcctgatgtg ccgttggcta attaacgatg tctcaagcca
    33421 ggtgtccagt gtcagaaacg gcagtgagac gctggcgaaa ggcaccgatg aactgaacga
    33481 acatacccag cagacagttg ataacgttca gcaaacggtg gcgaccatga accaaatggc
    33541 ggcgtcggtg aaacagaact ctgccacagc gtcggctgcc gataaactgt caatcaccgc
    33601 cagtaatgcg gcagtgcagg gcggggaggc gatgaccacg gtgatcaaga caatggacga
    33661 tatcgccgac agtacccagc gcattggcac cattacttcg ctgattaacg atattgcgtt
    33721 tcagaccaat attctggccc tgaatgcggc ggtggaagcg gcgcgtgccg gcgaacaggg
    33781 caaaggtttt gcagtggtgg caggggaagt gcgtcattta gccagccgca gcgccaatgc
    33841 tgccaacgat attcgcaagc tgattgatgc cagtgctgat aaggtgcaat ccggttcgca
    33901 gcaggtacac gccgccggac ggacgatgga agatattgtg gcacaggtga aaaacgtcac
    33961 ccaattgatc gcccagatta gccattcaac gctggaacag gccgatgggc tttccagcct
    34021 gacccgtgca gtggatgagc ttaacctgat cacccagaaa aatgccgagc tggtggaaga
    34081 gagtgcgcag gtgtcggcga tggtgaaaca ccgcgccagc cgactggaag acgcggtgac
    34141 ggtgctgcat taatcggtat gccggatccg gcggttggag cacaatgcct gatgcgatgc
    34201 tggcgcatct tatcaggcct acggggtatg tagggtacgc cgcatccggc aatcggcgat
    34261 taatccagtt gcgtaatatc aaaagcggcg cggtcgatca ccgcaatgat cttttttatt
    34321 tgtgcatcag tgatatcgct ctggttgacg cgtaaatcca gcaccgcttt aaaattatcc
    34381 agcgcccgct tcatttgcgg gttctggcgc agcgcagcac caacgcaacg cgctttgatg
    34441 cgttcttcaa tcatctccac ctgttcgcgg ttttcttcca gccactgcgc gccttgttcg
    34501 gtcagcgcaa tctgcttctt accaccttcc tcttcgcgga tggtaatcag cgactgctcc
    34561 tgcagaaaat ccagcgtcgg gtagatgacg cccgggcttg gggtgtaatt cccctgggtt
    34621 agattctcaa tcgctttaat caattcgtaa ccgtggctgt catcgcgcga gagaatatcc
    34681 agaatcacca ggcgcaattc accgtgacca aagaaacgtt gccgacgacc gccgccgcga
    34741 ccgtggcgac caccgcagca ttgaccgtgt tcatgctggt gaccgtgtcc gcagtgctcg
    34801 tgttctgact tctcaccttt gcagcagccc tcatggcgtg gctggccttc atgtttacaa
    34861 cacccttcgt gatgatggct catatcagcc tccttttgct taattagata tatctaattt
    34921 atatctgata tgagcactct tgcaagaata aaaatgattt ttaaatgtgg actcatctca
    34981 cacgtaggcc ggataaggcg ttcacgccgc atccggcgtt gagtaatgat gcctgagaag
    35041 aggcggttcg tcttatcaga ctatagacat atcagttcta caaatcgc
//
LOCUS       DAZFFG010000035        34999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFFG010000035 DAZFFG010000000
VERSION     DAZFFG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.035"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            61..801
                     /gene="yiaT"
                     /locus_tag="ACHJ7J_002117"
     CDS             61..801
                     /gene="yiaT"
                     /locus_tag="ACHJ7J_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO5000354.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(931..2901)
                     /locus_tag="ACHJ7J_002118"
     CDS             complement(931..2901)
                     /locus_tag="ACHJ7J_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO5000355.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKVPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKREQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2912..4312)
                     /locus_tag="ACHJ7J_002119"
     CDS             complement(2912..4312)
                     /locus_tag="ACHJ7J_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5000356.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4538..5353
                     /locus_tag="ACHJ7J_002120"
     CDS             4538..5353
                     /locus_tag="ACHJ7J_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5000357.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            5355..5495
                     /locus_tag="ACHJ7J_002121"
                     /pseudo
     CDS             5355..5495
                     /locus_tag="ACHJ7J_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            5597..6070
                     /gene="ysaA"
                     /locus_tag="ACHJ7J_002122"
     CDS             5597..6070
                     /gene="ysaA"
                     /locus_tag="ACHJ7J_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5000358.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(6221..7474)
                     /gene="avtA"
                     /locus_tag="ACHJ7J_002123"
     CDS             complement(6221..7474)
                     /gene="avtA"
                     /locus_tag="ACHJ7J_002123"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO5000359.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7652..9682)
                     /gene="malS"
                     /locus_tag="ACHJ7J_002124"
     CDS             complement(7652..9682)
                     /gene="malS"
                     /locus_tag="ACHJ7J_002124"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5000360.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFSEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKIGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            10002..10826
                     /gene="bax"
                     /locus_tag="ACHJ7J_002125"
     CDS             10002..10826
                     /gene="bax"
                     /locus_tag="ACHJ7J_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO5000361.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(10934..12112)
                     /gene="xylR"
                     /locus_tag="ACHJ7J_002126"
     CDS             complement(10934..12112)
                     /gene="xylR"
                     /locus_tag="ACHJ7J_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO5000362.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFCQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(12190..13371)
                     /gene="xylH"
                     /locus_tag="ACHJ7J_002127"
     CDS             complement(12190..13371)
                     /gene="xylH"
                     /locus_tag="ACHJ7J_002127"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO5000363.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(13349..14890)
                     /gene="xylG"
                     /locus_tag="ACHJ7J_002128"
     CDS             complement(13349..14890)
                     /gene="xylG"
                     /locus_tag="ACHJ7J_002128"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO5000364.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(14968..15960)
                     /gene="xylF"
                     /locus_tag="ACHJ7J_002129"
     CDS             complement(14968..15960)
                     /gene="xylF"
                     /locus_tag="ACHJ7J_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5000365.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            16326..17648
                     /gene="xylA"
                     /locus_tag="ACHJ7J_002130"
     CDS             16326..17648
                     /gene="xylA"
                     /locus_tag="ACHJ7J_002130"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO5000366.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            17720..19174
                     /gene="xylB"
                     /locus_tag="ACHJ7J_002131"
     CDS             17720..19174
                     /gene="xylB"
                     /locus_tag="ACHJ7J_002131"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO5000367.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            19343..19684
                     /gene="yiaB"
                     /locus_tag="ACHJ7J_002132"
     CDS             19343..19684
                     /gene="yiaB"
                     /locus_tag="ACHJ7J_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO5000368.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFFGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            19730..20167
                     /gene="yiaA"
                     /locus_tag="ACHJ7J_002133"
     CDS             19730..20167
                     /gene="yiaA"
                     /locus_tag="ACHJ7J_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO5000369.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQRTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(20209..21204)
                     /gene="wecH"
                     /locus_tag="ACHJ7J_002134"
     CDS             complement(20209..21204)
                     /gene="wecH"
                     /locus_tag="ACHJ7J_002134"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO5000370.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRRNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            21379..21678
                     /gene="ysaB"
                     /locus_tag="ACHJ7J_002135"
     CDS             21379..21678
                     /gene="ysaB"
                     /locus_tag="ACHJ7J_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO5000371.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            21773..22684
                     /gene="glyQ"
                     /locus_tag="ACHJ7J_002136"
     CDS             21773..22684
                     /gene="glyQ"
                     /locus_tag="ACHJ7J_002136"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO5000372.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            22694..24763
                     /gene="glyS"
                     /locus_tag="ACHJ7J_002137"
     CDS             22694..24763
                     /gene="glyS"
                     /locus_tag="ACHJ7J_002137"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO5000373.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            25086..25238
                     /gene="hokA"
                     /locus_tag="ACHJ7J_002138"
     CDS             25086..25238
                     /gene="hokA"
                     /locus_tag="ACHJ7J_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO5000374.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(25215..25286)
                     /locus_tag="ACHJ7J_002139"
     CDS             complement(25215..25286)
                     /locus_tag="ACHJ7J_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000375.1"
                     /translation="MRNAVKKAGITSRRRLLLFQIAG"
     gene            complement(25426..25638)
                     /gene="cspA"
                     /locus_tag="ACHJ7J_002140"
     CDS             complement(25426..25638)
                     /gene="cspA"
                     /locus_tag="ACHJ7J_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO5000376.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(25919..26209)
                     /gene="yiaG"
                     /locus_tag="ACHJ7J_002141"
     CDS             complement(25919..26209)
                     /gene="yiaG"
                     /locus_tag="ACHJ7J_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO5000377.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            26643..27353
                     /gene="yiaF"
                     /locus_tag="ACHJ7J_002142"
     CDS             26643..27353
                     /gene="yiaF"
                     /locus_tag="ACHJ7J_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO5000378.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(27403..28377)
                     /gene="ghrB"
                     /locus_tag="ACHJ7J_002143"
     CDS             complement(27403..28377)
                     /gene="ghrB"
                     /locus_tag="ACHJ7J_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO5000379.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALMLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(28481..29140)
                     /gene="yiaD"
                     /locus_tag="ACHJ7J_002144"
     CDS             complement(28481..29140)
                     /gene="yiaD"
                     /locus_tag="ACHJ7J_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO5000380.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            29293..31626
                     /gene="bisC"
                     /locus_tag="ACHJ7J_002145"
                     /pseudo
     CDS             29293..31626
                     /gene="bisC"
                     /locus_tag="ACHJ7J_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
     gene            complement(31595..32035)
                     /gene="yiaC"
                     /locus_tag="ACHJ7J_002146"
     CDS             complement(31595..32035)
                     /gene="yiaC"
                     /locus_tag="ACHJ7J_002146"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5000381.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRECIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(32032..32595)
                     /gene="tag"
                     /locus_tag="ACHJ7J_002147"
     CDS             complement(32032..32595)
                     /gene="tag"
                     /locus_tag="ACHJ7J_002147"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO5000382.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDIERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            32753..33451
                     /gene="yhjY"
                     /locus_tag="ACHJ7J_002148"
     CDS             32753..33451
                     /gene="yhjY"
                     /locus_tag="ACHJ7J_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5000383.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGSWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            33680..34882
                     /gene="yhjX"
                     /locus_tag="ACHJ7J_002149"
     CDS             33680..34882
                     /gene="yhjX"
                     /locus_tag="ACHJ7J_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5000384.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         8574 a         8563 c         9008 g         8854 t
ORIGIN      
        1 aaattgtaag cattactatg ccgactcttt tttatcttat tttaaaatgg aataccggac
       61 atgttaatta accgcaatat tgtggcgtta tttgcgttgc cttttatggc aagcgcaact
      121 gcttctgaat tatccattgg tgctggtgcg gcttataatg aatcgcctta tcgcggttat
      181 aatgaaaata caaaggcaat tcccctgatt agttatgaag gtgattcttt ttatgttcgt
      241 cagaccacgt taggttttat tctgtcgcaa agtgaaaaaa atgaacttag cctgaccgca
      301 tcctggatgc cgctggaatt tgatcctgcc gataatgacg attatgccat gcaacagctt
      361 gataagcgtg atagtacggc tatggcgggg gttgcctggt atcaccacga gcgttgggga
      421 accgtgaaag cctctgcagc tgcggacgtt ctggataaca gcaacggctg ggtgggggag
      481 ctatcggtat tccacaaaat gcagataggt cgtctgtcgc tgacacctgc gctgggcgtt
      541 ctctattatg acgagaattt cagtgactat tactatggca tttcagagag tgagtcccgt
      601 cgtagcggtc tggcaagtta ttccgcgcag gatgcctggg tgccctatgt cagcctgacg
      661 gcaaaatacc cgataggaga gcacgtcgta ttgatggcga gcgcaggata cagcgagctg
      721 ccggaagaga ttaccgacag cccgatgatt gatcgtaatg agagttttac ctttgttacc
      781 ggcgtgagct ggcgttttta attcaccggt ggatgtcggt gcggctggcc tacgctctcg
      841 caccgacttg tatgcctgat aagacgtgtt aagcgtcgca tcaggcaatg tgctccttac
      901 gttggatgcg gcgcgagtgc cttatccaac ctacggatgg caatgctttt cctcattcac
      961 ccagatccgc atttcgcctt caccccggtt ggcccagcta aaccacggga taaaggtcag
     1021 agtttgcgtc tggcgtttgg ctggcgcgct gtcgtaatgc cacagcggtt gctgctctgg
     1081 attgctctgt tcataccggt aacccggtgc ctggattaag atcttatggc taaacaatcc
     1141 cttgccttca aatgtcgtaa atggtgcatc ggtgggcagc cacagattat gcagtgactc
     1201 gccgttgtcg gcctgttcca ggcaatacac cagcgggccg cgctgaatcg ccacttttcc
     1261 ggcgacgtga cgcaccagcg ggttaccgta aacgcggcgt accggcatcg gcaaagtcag
     1321 attcagcgta tcgccctcct gccattcgcg ggtaatgtgc aaataccctt tacgaatatc
     1381 ctgctcgacc tcttccccat tcaatatgat ctgcggctgt gtgcaccagt ccggcagacg
     1441 taaagccagc gtatgacgta ccggctgggg cgattcaacc gcaatcgtca cctgctcctg
     1501 ccacggatag ttcccgctaa cccgcaggcg cagcgtgcca ttttctaccg gcacttccat
     1561 gctgtttcct gcgtatatgt tgatatacaa cgcatcttca cgcggcgtgt agagataatg
     1621 accaatcgag gtcagcacgc gggcgatatt tggcggacaa caagcgcagc caaaccaacg
     1681 ctggcggatc ggtttaacgt gatcgtatat atggttgaat ttcagcgatt ttggatgtac
     1741 ttccagcgga ttcacataga agaaatgttt gccatccagc gccatgccgc cgagcacggt
     1801 gttgtacagt gcgcgctcca tcacatcggc atattgactg tcgccttcca tttccagcat
     1861 tcgtcgggcg aacatcatca ggccgatgga ggcgcaactt tcagcgtaaa ccgtgtcatt
     1921 cggcagatcg taatcgctag tgaacgcttc gccgctgctt tgcgagccaa tgccgccggt
     1981 aatatataac tgacgctggg ccatattgtt ccacagccgc aggcagtcct gacgcttgct
     2041 gtcatcgtga cttaaacgcg cgagatgcgc gacgccggtc atcagataga caaaacgtac
     2101 cgcgtgaccg atggcggttt gctgttgcgc aaggggcaaa tgtgcctggc tgtaggcttt
     2161 gtctttcacc atccacgccg ggccgtaggt gtgccagtgc gatgtctgct cgcgcttttc
     2221 atattcctgg tcgtaatagt gcggttgcgc accacgctgt tcgacaaaat agttcgtcag
     2281 tgccaggtag cgcggctctt cggtcacttc atacagacgc atcagtgcca gttcaatttc
     2341 cgggtgacca gggtaaccgt gtaacttact ttcatctgga ccaaatacgc tgtcgatatg
     2401 atcggccaga cggcaaacca cttccagcaa gcgccgcttg cccgtggcct ggaagaaggc
     2461 gactccggct tcaatcagat gaccggcgca gtaaagttca tgacactccg ccagattgct
     2521 ccagcgttct tcgggtactt ttaccgtaaa gtaagtattg agatagccgt cttcgcattg
     2581 ggcggaggcg atcagttcga ttacctcgtc ggcggttttt tccagttcgg cgtccggctt
     2641 ctggcacagc gaccaggcta ccgcttccag ccatttggcg acgtcgctgt cctgaaacac
     2701 catcccgtaa aattcaccct cctgaagtcc ggcggcaata cgaaagtttt caatcgcatg
     2761 gctgggttcc gcttctggga tacgatcgtt caaggcatcc cactgataag gaatcaccac
     2821 gtcgcggacc agttgttggt actgaccgag gaacggatcg ctgaccgtca gtttatgcag
     2881 atcgacttcc gaaatgttca tcttgtgctc cttaaggctg tacatgacgc tgacgcaggt
     2941 cggtagctat gcgtgacatc ataggattat tgagtttgca aaagcggatc gcccctgcca
     3001 gcagcaggtg gaatagcgca ggcagtaatg tttccatcgc cgcgatcccg ccaagcgaac
     3061 tggcggtctg gttttccacg ccaggctggt aggcgacaaa aataaacagc aggctgatga
     3121 tcccggcgct ggaggcccag gccagcttga tgaaaaacag attgaaggcg aagttcatgc
     3181 cggaagaacg tacgcgggtt ttccactcgc cgtagtcatc ggcaaaggcc atcaatgaga
     3241 agtgcagtgg caatgtgaag ccaagaatca cgccattacc gaggatcacc gccagccaca
     3301 gcgtttgata agccgggccg gagggcagga accacatcaa caccgccagt gcagcgagga
     3361 gcaggttggt gtagtaataa attttgacgg tatcgaaacg ccgcgttaac gggctgacaa
     3421 tcaccgagcc gataatggag gcgaaggtga ccatggtgaa gaacagcgac gtgtagcccg
     3481 tgctgccttg taagacgtag gtaatgaagt acatataccc accgccgcga atgttaaaga
     3541 cgttaatcag caggaaagac atgaccagca tcagcagcag ttggtcgttg ttccgcagcc
     3601 cggcaagatg ttcgcgcagg gtaaatttcc ccattgtgga gagtggcacc cgttcacgaa
     3661 cccagaaaaa acagcacagg aacatcacca cggcaatggc gcacagcacg ccgacgccca
     3721 attgatagcc gcgcgcggca ttaccctgac cgaagagatc taccatccac ggtaagccta
     3781 ccgaaaccag aaatcccgct acgccacaga gaacgaatcg ccaggactgg caggagatca
     3841 cttcattgtg gcgggtggtc atggtgttga tcagcgcgca gtacggtacg ttgatggcgg
     3901 tataaccgac tgaaaggagc aggtaagtac cgaacgccca ggcgattttt actcccatgc
     3961 tggcatctgg cacggtgaac gtcaggatgc cgataatgcc gatgggaatg gcaatccata
     4021 gctgccacgg acgaaagcgc ccccagcgac tttgcgtccg gtcggcaatg acgcccatca
     4081 ccgggtcgga gatggcatca aacacgcgca gtgcaataaa cagcgtgcca accagcgccg
     4141 gagttaatcc aaaaacgtcg gtgtagaaaa aggtgagaaa attcatgatc aggcaagtta
     4201 ttacggtgcc gcctgcgtca cccaggccgt agcctatttt ttcccgtaca gataacgcat
     4261 cgtcaatttt acctttagcg atatcagcgg tagtaatcgg tgtagaggtc atagatattc
     4321 ctcgtgaata gggagcaatt tttagtttta ttgaaatgac ctgattaatt ctgccgtgaa
     4381 cgggaatatc aacaaggcga gaaaagagta aaaaaaggtg acgattccga cctgaattta
     4441 aaagtgtgaa atggatcagg tgaaattaat gtttttgatt aataatcaaa aggtaatgtg
     4501 tttaatcatt aatgaataag cgggaaatta catatcaatg ctcgaattat ccatgacgtt
     4561 accgattaaa gtgcaaaatg gtggattatt tatttcccgt ggcgtaggga ggcatcctgc
     4621 aaggcggctg gagtcatggg aaattatctt tgtcgaaaaa ggtcgattaa cgattcagga
     4681 agaagaacgg atatttctgg tcgatgcggg agagagttta ttactctggc cgaaccgaca
     4741 gcatgttggg gtggaggatt ttcccgccga tctcaaattt tactggctgc attttgaggt
     4801 taaagcgccg gacaacgacc ctcgctggct tactcatctt agcgtgccgc aacaaacgaa
     4861 ggttgtcgat ccgcaggcgc tggtggccct gtttcgccag ttcttaagcg aacaggaaaa
     4921 gcatcaacgc actccggcgc tggagtttat tgtgctgctc attttgcagc agctcacccg
     4981 tgacgcgcgt cttgatgaac aggccgatgg cgcaggcacc acccttgcct ggaaagcgca
     5041 gaaactgatc cgcacgcaat atcatcgccc gctttctacg gcgcttctgg cccgcgagct
     5101 gtactgcaat gccgattatc ttgggcgcgt ctaccggcgg gtgtttcatc tgacgttaac
     5161 agaagcaatc catcgccagc gggtgcgcga agccgaaaag ctgttaatta gcgaagcgcg
     5221 atcgctcacg gaagtggcgg cgttgtgcgg gtttaatgac gttggctatt tccggcagat
     5281 ttttcgcaaa cacaccggtt taacgccagc agcgtggaag cggcgttaca gcaaggaaca
     5341 tatcaattcg tagtgccgtg tcgatttcgc tttcgacatc acgtctgaaa caggtgggag
     5401 agaaaaatct cctgaaacct ctgcgtgaaa ccgcgcaggc tatttctaat gaactagggt
     5461 ttactgtcag ggacgacctg ggcgcgatca cataacgctt tggacaagtg ccaaaacttt
     5521 aacatttcct tcgttggatc aaagcagtag gggcgcgttc tctggcactc tgctgtttta
     5581 gtgcaaaggg gtgatcatga accggtttat tattgcggat gcgacgaaat gtatcggttg
     5641 ccgtacctgt gaagtggctt gcgcagtgtc gcatcaggag aatcaggatt gcgctgcgtt
     5701 gtcaccagac gagtttattt cccgtattcg tgttattaaa gaccacagct ggaccacggc
     5761 agtagcctgt catcagtgtg aagatgcacc gtgcgcgaat gtctgccctg ttgacgcgat
     5821 aagccgcgaa catgggcata ttttcgttga acaatcacgt tgcattggct gtaaaagctg
     5881 tatgctggct tgcccgtttg gtgcgatgga ggtcgtttct tcgcgcaaaa aggcgagggc
     5941 gattaagtgc gatctgtgct ggcatcgggc gacgggaccg gcctgtgttg aagcctgccc
     6001 gacaaaggcg ttgcagtgca tggatgtcga gaaagtgcag cggcatcggc tacggcagca
     6061 gcctgtttga aacgttttcc ggcgtttctc aacgccggaa tagtgcgctt tgtttatgcc
     6121 agatgcggcg taaacgcctt atccggccta taaattcttc caaattcaat atattgcaga
     6181 aatcatgtag gcctgataag cgtagcgcat caggcaattt ttagtgactt tcagcccagg
     6241 ctctttctat ctcttccgcc agaatcttca cccccgcctc aattttctcc ggctctggta
     6301 cgtagttcat gcgcatacat tgatgcgtat gcggccacgg tttatccagc cctgggaaga
     6361 agttgtgccc cggcaccatc agcacgccgc gtgctttcag gcgctggtag agctgctcgg
     6421 tcgtaatggg caaatcctta aaccatagcc agaggaaaat ggctccttcc ggtttatgaa
     6481 tcaggcagcg attttccggt aaatagcggc gaatgatggc gatagtttcc tgaacacgct
     6541 ggtagtaaaa cggtttgatg actgtttcag acaggcgcag cagatcgtta cgcttaatca
     6601 tttcacacat catcgccgga ccaataccgc caggtgccag gctgataatg ccgttcatat
     6661 tggtgatggc ggtgatgatt ttttcattgg cgatgataat gccgcagcgg gagccaggta
     6721 gacccagctt ggaaagactc atgcacagca cgatattcgg attccatagc gggcgcgctt
     6781 cactgaagat gatacccggg aacgggacgc cataagcgtt atcaatcacc agcggaatgc
     6841 cgtgttgatt cgccagcgcg tcaagcttca gcaactcttc gtcagtaatc acattgcctg
     6901 ttggattcgt cggccgggag acgcaaatca tcccggtttc ttcgccaata tgcagatgct
     6961 caaaatcgac gtggtattta aactggcctt caggcagcag ttcaatattc ggacgcgcag
     7021 agacaaacag atcttcttcc agtccggcgt cagcgtagcc aatgtattcc ggtgccagcg
     7081 ggaacagcac ttttttgacc cgaccatcgg cacggcgtcc ggcaaacagg ttaaataagt
     7141 agaaaaacgc gctctggctg ccgtttgtta gtgcaatatt ctgtggttcg atatcccaac
     7201 ccaacttctc gcgcagcatt ccggcaagca gtgtgagtag ctccgttttc ccctgtggac
     7261 cgtcgtagtt acacagtgca tcagtcgctt tgccactttc cagcatgtcg gtcagtagcg
     7321 tctggaagta gtcctgcatt tccgggatct gcgccggatt accgccgccg agcataatcg
     7381 cgccaggcgt gcgtaaaccg tcgttcagat cttccatcag cagcgtaatg ccggagtggc
     7441 gggtaaattt gtcaccaaaa agggagaatg tcatagcggg atatctgtcg aactctgtag
     7501 caaggaaggt aacaataacg ctacatttgc tggggtgcaa atcggtctgt tgaagagtga
     7561 gcggtgcttt atcctgcaac gctgattagg gctgacattt tatcctggtt ggcggcttcc
     7621 cgaggtactg acgaacggga agccggaaaa gtcactgttg cccagcccag atgaccagca
     7681 ctttatcgtc gccatgctca cgaacaaagc cgtagccctg cttcagcgaa agtgtcgttt
     7741 gtttgcccgc gccaattgcg ggatggcggg cgcggaactg gctgattttc tgccagtgcg
     7801 cgacgctggc ggcagattta ccgctaacat cctgccagtt catatccgaa cgtgtacctt
     7861 gcaacggatc agaacctgta ggaccgaacg gacgcgagga ttcatcacca taaaagattt
     7921 gtaccgcgcc tggcgctaat agtaataact ctgctgcttt gtcgccccct tcacggaaca
     7981 ggcgggtatc atgcgacgag aggtagctca acacgttgaa accctgcaat ttctccgcca
     8041 tttgctgcca ggtcgtatcc atctgcgcca gacaatcgac tgctttcgcc gcctgctcct
     8101 gataatcgaa attgatcatc gcatcgaagc cgtggcgata gtagtcactt tgcatcacgc
     8161 cgtggcccca ggcttcaccg gtcatccaga aaggtttgtc atctaatgct ttgtcggggt
     8221 tagctttttt ccattcgcga agcgcggcgc tggcttcggt tttcagttgc tgccaggcgg
     8281 gcaactcaac atgtttggcg gtatcgaccc gaaaaccatc aatcccatag tcgcggaccc
     8341 actgacttaa ccagtgggtt aagtaatcgc gaggtgtaaa gccttcgatg actttagcgt
     8401 gagtatccgt tttgttttta tagaacaccg gcagaccaga agcggtcgtt gattcggttt
     8461 tgatatccgg caaaaaggcc agcgacatag tgagatcgtc aaatccagga ttgtcgtaat
     8521 cgccgatatc cgttctgatc cagttttttc cccaccattt atcccagcct attttgtcgc
     8581 tgaaattaat gtaatcgtta aagctatgcc aggtttgccc ggcggcaggt ttccagtcac
     8641 tccagcgttc acccagcgtt tttttcagct cgtcaccaga aagatataac gcgccaaact
     8701 gatactcctg catatccgcc agcgtggcat agccggtgtg gttcatcacg acatcaaaga
     8761 gaatacgaat accgcgctga tgcgcgctat caaccagcgt ccgtagatcg gcttcgctgc
     8821 ccatattggc atcaagattc gtccagtcct gtgtgtaata accgtggtag gcataatgcg
     8881 ggaaatcacc ttttgtaccg ccgccgaccc agccgtgaat ttgctcaaat ggggcgctta
     8941 tccacaaagc attgacgccc agctgctgga gataatccag tttgttggtc aggccgcgta
     9001 aatcgccgcc gtgaaaagta cctatttctg ccataccgtc tttatgacgt ccgtaactct
     9061 ggtcattact gggatcgccg ttttcgaaac gatctgtcag cacaaagtaa accgtggcgt
     9121 tatgccagtc gaaaggggca gaggcgtcag tttctgcccg ttccagcagg agtaaaccgt
     9181 tgctggtggc agcgggttgt aacgttattt gaccattctt cactatcgca atttgctggc
     9241 tgtaataatc ccgtacggcg gctccttccg agaaagtggc gctgacatcc actgtgagcg
     9301 gtaatccatc ccatttcggg cattcacgga ccaggcttgc taccggttcg gcggcgttct
     9361 ggatggaaat catcaatgtt ggcgtaccgg agcgggtgtc tatttgcagc gtatattcgc
     9421 cgtccctgaa caatcgccat tgaggcggcg tgttgctaca aggttgcagg gaaagcatct
     9481 gattgagttt tatcgcatcc gcaggctgcc agcactgttg gtcaaaattt agcgttagtg
     9541 gacgcgtacc tttgggcaac tgcgcgtggc tgacaaatgt tccagttccc tgttcactaa
     9601 aggcagggaa ccccggagaa gtccagctgg cggcaacggc gaagccagga aggagtgtca
     9661 gaaaacaggc ggcgagtttc atggcggatg agtccttatt gctgcgattt cagacagttt
     9721 gccagcgata agccagacaa aactcatccc tgagcggtaa gtccacagga tgagaagcaa
     9781 gggtgagcga tctcgcgcaa aaaacggctg aattttgcga taaaccccac attttctgcg
     9841 atttagcgcc aatctgaatc gttaacacgt gacatagttt cagatttgga ctattccttg
     9901 gggtgctctt gacagctatt tttgatatga tttgagattc aactctcaaa tttgtgaaaa
     9961 atataaggtg ttggaatgat taaatccgac caggagacct gatgattttg actcccatac
    10021 gacgatatgg ggcgatgatt cttatgttac tcactctggt gttttcgagt gaggtgttag
    10081 cgaagacgca cacaacaaca gcgagtcaaa agtcccactt aactaaagct agtaataaac
    10141 aggtaagcag taaacaagag tattctcgca atagtgcaaa gagtagttca cttcctgatt
    10201 tgcgaaaata cccttccgga acaccaagga aaaaggcgtt tctccggacc gtaatgcctt
    10261 acattaccag ccaaaatgca gccattactg cggaacgtaa ctggctgatt tcaaaacagt
    10321 atcagggcca atggtcacct gctgagcgtg cgcgtctgaa agacatcgcc aaacgctaca
    10381 aggtgaagtg gtctggtaat acgcgaaaaa tcccgtggaa taccttgctt gaacgcgtag
    10441 acattatccc taccagtatg gtggcgacga tggctgcagc agaaagcggt tggggaacgt
    10501 cgaagctggc gcgcaacaac aacaatcttt tcggcatgaa atgcatgaaa ggacgttgta
    10561 ccaatgcgcc aggtaaagtg aaagggtact cacagtttag ttctgtcaaa gaatcggtga
    10621 gcgcctatgt cactaacctc aatacgcatc cggcttactc ttcgttccgt aaatcgcgtg
    10681 cacagctgcg taaagcggat caggaagtga ctgccacagc gatgattcac aagctgaagg
    10741 gctactcgac caaagggaag agttataaca actacctgtt cgcaatgtac caggataacc
    10801 aacggttaat cgcggcgcat atgtaattgt atttcctcga gccttatccg acttgtcggt
    10861 cggataaggc ttttgatcgc atcaggtggt tgtgctgagt tacctgatgc gacgccgaca
    10921 attctcatca tcgctacaac atgacctcgc tatttacatc gcgatactct tttggcgtcg
    10981 tgtcatatgc ttttttaaaa acagagtaga aatattgcag cgatggataa ccgcacattt
    11041 gcgatatctc attgatcgac aaggtggttg aaatcagcag actgcgcgct ttctccagct
    11101 tctcggcatg aatcatggca tggatggttt cacccacctc ttctttaaaa cgcttctcaa
    11161 gattggagcg cgagatcccg accgcatcga gtacctgatc cactttaatc cctttacagg
    11221 cgtgattacg aatgtaatgc atggcctgaa taacggcggg atcggtcagc gaacggtaat
    11281 ctgttgagcg ccgttcaatg acgcgaactg gagggaccaa aatccgctgt agcggcattt
    11341 cttctttatc taataatcga tgcaacagtt ttgccgcctg atagcccatt tgccgcgcgc
    11401 cctgagcgac cgaagaaagg gcgacacgcg acagatagcg ggtcagttct tcgttatcga
    11461 tgccaatcac gcataatttt tccggtacgg gaatgtgtag atgttcacat acttgcagaa
    11521 tatgccgcgc ccgggcgtca gtaacggcaa taatcccggt ttgcggtggc agcgtttgta
    11581 gccagtctgc cagccgattt tgcgcgtgtt gccagttctc tggtgcggtt tctaacccct
    11641 gataaaccac tccgcgatac ttttcttcgg cgacaagctg acaaaatgca tattcgcgtt
    11701 cagtggccca acgtttgccg cttgattccg gaagaccata aaaagcaaag cgattaacgc
    11761 ctttctcttt taaatgcaaa aatgcgcttt caaccagcgc atagttatcg gtggcaatgt
    11821 aatgaacggg tgggtaactt tctgcaaggt gatacgagcc gccaacccca acaatgggga
    11881 cgtcgacatc agccagcgct tgctcgatct gtttgtcgtc gaagtcggca atgacgccat
    11941 ctcctaacca gtccttgatt ttatcaatgc gggcgcggaa atcttcttca atgaaaatat
    12001 cccattccga ttgtgacgcc tgtaaatatt cccctacgcc ttctactacc tgccggtcat
    12061 aggctttatt ggcattgaac agtaatgtga tgcggtgacg tttagtaaac atggttcttt
    12121 tcctgctgaa tcatgcaaaa actcaaaacc ggtaatacgt taccggcttt gagaaaattt
    12181 ttatcaaaat caagaacggc gtttggttgc ggagtccatc catactgcca gcaacagaat
    12241 cgcaccttta acgatatact gccagaaggt cggtacatcc atcatactca taccgttatc
    12301 cagtgaagcc atgatgaatg cccccattac cgctccagcc acgctcccga cgccgcctgc
    12361 cagactggtg ccgccaatca cgcatgctgc aattgcgtcc agctcggcga tattccccgc
    12421 agaaggtgaa ccagcgccaa gtcgagaact gaggattaat ccggcgatgg ctaccattaa
    12481 tccgttaata gcgaacacgg caagtttggt gcgttcaacg ttaattccgg agagacgtgc
    12541 tgcttccaga ttgccgccga tggcataaat gcgtcgtcca aatgctgtcc gcgttgccat
    12601 aaacattccg ccgagtaaca gcaacgtcag cagcagaaca ggagtgggaa cgccacggta
    12661 atcattcaac agccagattg cgcctaatac gatgatagcg gttaaagcct ggcgaccgac
    12721 tactgcggta gaggccggag actgcaaacc caaagcctga cggcgcattc ttccgcgcca
    12781 ttgccagcca acaaaagcca ttaagccaag cgcgccaatg atgaagccgg tgctggcggg
    12841 gagatagctt tgcccaattt gtgacatcgc ggcgctggtg ggggatacag ttgtgccgtt
    12901 ggtgatgcca atgagtatgc cgcgaaatgc caacatgccc gcgagagtga caataaatga
    12961 agggactttg cggtacgcga cccaccatcc gttccaggca ccgagaagca gtcccagaac
    13021 caacgtcaca atgatggtaa gtggcaaagg ccagcctagc cagacgtcac aaatcgccgc
    13081 gacgccacct aatagcccca tcattgagcc gacggaaagg tcgatttcag cagaaattat
    13141 gacgaacacc attcctaccg cgaggatgcc ggtaatcgcg gtctggcgta acaggttgga
    13201 gacgttacgg gcgcttaagt aggcaccatc ggtggtccag gtaaagaaca gcatgattgc
    13261 gatgatagct gcaatcatca cgaagacctg caaattcagt gatttcagcc cggagaagcc
    13321 accggatgtc ggtacggcca attttacttc agacggattg cttttcgaca tgatgttcgc
    13381 tcctcaatgc ggcttccatc acctgctcct gagtcaggtt atgatttatc aggttggctt
    13441 ttagtttccc ttcatgcatc accagtacac gatcgctaag gccgagcact tcaggtaatt
    13501 cggaagagat gacaataacg gcaataccct gctggacgag ttggttaatt aatttgtaga
    13561 tctcatattt cgcgccaata tcaatacccc tggtaggttc atcaagaatg agaatgcgcg
    13621 ggttgagtaa cagacagcgg gcgaggatcg ctttttgctg attgccgccg ctcaaacgtc
    13681 caatagcaag gtcgggggac gacgttttaa ctttgagttg ctggattgat tccagaatac
    13741 atttttgctc tgccgcgtca tcaagctggc taatgccacc ggtaaattta ttgagtgcgg
    13801 cgagggtaat atttttacca accgccatta ccggaacgat gccgtcgcgc tttctgtctt
    13861 cggggaccat cgcaatcccc tgggcgatgg cttgctgaca gttacgaata tctacctgtt
    13921 tgccatcaat ataaattttt ccttcccatt gtccgggcca cacgccaaac aggcactgaa
    13981 tggtctcggt acgtccggca ccaacgagtc cggcaatacc cagtatttcg ccacgtttca
    14041 gggaaaacga gacatcatta actcgtttaa tatgacgatt aaccggatgc catgccgtca
    14101 gatgttcaat acgtaatatt tcatctccgg tggtatgtgg ttcattaggg taaagcgcgg
    14161 ttaactctcg cccgaccatc atggtgataa tatcgtcttc actcattccg gcagcatcac
    14221 gcgtaccaat gtgctgtccg tcgcgaataa cgcaaatcgt atcggaaatc gctttgactt
    14281 cgttgagttt gtgcgaaata taaatacagg cgataccgtg ctgttgtaga tcgcgaataa
    14341 tatccagtaa aaccgacgtt tcctgctcag ttaatgaggc tgtcggttca tcgagaatta
    14401 acaagcgcac ctgtttatta agtgccttgg caatttcaac cagttgttgt tgcccaagcc
    14461 ctaaatcgcc aacgcgggta tcaggtgaaa tggataaact gacctgtgcg agcagcttct
    14521 gacagcgtag cgtcatcagg tcataatcca taatgccatt gtgggttatt tcgttaccca
    14581 ggaagatatt ttccagcacg gtcaattctt tcaccagggc caattcctgg tgaatgatgg
    14641 cgataccttt gcgttcggta tcgcggatgt gactcgcctg aatctcttct cccgcaaaaa
    14701 taatttcgcc ttcgtaggag ccatggggat aaataccaca cagcactttc atcagagttg
    14761 atttaccaga cccattttcc ccacagagtg agacgatttc gccagcattt aaccgcaagc
    14821 tgacgttatc aatcgccttc acactgccga aggttttggt aatgttcttc atttcaagta
    14881 gataaggcat aacgactcca cctaagccaa ttcattcacg cggcatggag agaaatcacg
    14941 cccccgctcc gcgctggggc gtaacgctta cagctcgctc tctttgtgga atccgtcttt
    15001 aattaccgta tctttgatgt tgtttttatt cacatcgatc ggtgtcagga ggcgggaggg
    15061 gacatctttc aggccattat tcagtgaggt atctgctttt ggctcctgac cattgcccaa
    15121 ttcaacggca atttctgcgg cagtatttgc cagcaatgta ataggtttat ataccgtcat
    15181 agtttgcgta ccggcagcaa tacgtttaat acctgcgaga tccgcatcct ggccggaaat
    15241 tgctactttc cctgataaac cttgcgcgct taatgcctga attgccccac ctgcggtggc
    15301 atcgtttgag gcaactacag catcaatttt gttattattg gcggttagcg cgttttccat
    15361 aattttcaat gcgttttccg gtaaccagcc atcaacccat tggtcaccaa cgactttaat
    15421 ttttccggaa tcaacgtaag gttttaacac tttcatttgt ccggcgcgga acagcttagc
    15481 gttgttatct accggcgagc cgcccatcag gaagtaatta ccttgcggaa caatatcgac
    15541 cagggctttt gcctgcagtt caccgacttt ttcgttatcg aaagaaatat aaaaatcgat
    15601 atccgcatcg ttaatcatac ggtcgtaagc taatacttta atgccttctt gtttggcttc
    15661 ttttacaacg ttacttaata cctgaccgtt atacggaata atgacaagaa catcgacacc
    15721 ccggtttatc atgttttcaa tctgcgacat ttgtgtttct tcattgccat ttgcagactg
    15781 tacaaatact ttcgcgccga gagattctgc ctttttcaca aaaatatctc gatctttttg
    15841 ccagcgttca agacggagat catcaatcgc catacctatt ttgacttctt tggcgtgtgc
    15901 cgcaacgttg gtaagcagaa gtgaggtgca aagggtgagt agaatgttct ttattttcat
    15961 ggtgtagggc cttctgtagt tagaggacag ctttaataag taacaatcac cgcgataaac
    16021 gtaaccaatt tttagcaact aaacagggga aaacaattac agatttttat ctttcgatta
    16081 cgatttttgg tttatttctt gatttatgac cgagatctta cttttgttgc gcaattgtac
    16141 ttattgcatt tttctcttcg aggaattacc cagtttcatc attccatttt attttgcgag
    16201 cgagcgcaca cttgtgaatt atctcaatag cagtgtgaaa taacataatt gagcaactga
    16261 aagggagtgc ccaatattac gacatcatcc atcacccgcg gcattacctg attatggagt
    16321 tcaatatgca agcctatttt gaccagctcg atcgcgttcg ttatgaaggc tcaaaatcct
    16381 caaacccgtt agcattccgt cactacaatc ccgacgaact ggtgttgggc aagcgtatgg
    16441 aagagcactt gcgatttgcc gcctgctact ggcacacctt ctgctggaac ggggcggaca
    16501 tgtttggtgt gggggcgttt aatcgtccgt ggcagcagcc tggtgaggca ctggcgttgg
    16561 cgaagcgtaa agcagatgtc gcatttgagt ttttccacaa gttacatgtg ccattttatt
    16621 gcttccacga tgtggatgtt tcccctgagg gcgcgtcgtt aaaagagtac atcaataatt
    16681 ttgcgcaaat ggttgatgtc ctggcaggca agcaagaaga gagcggcgtg aagctgctgt
    16741 ggggaaccgc caactgcttt acaaaccctc gctacggcgc gggtgcggcg acgaacccag
    16801 atccagaagt cttcagctgg gcggcaacgc aagttgttac agcgatggaa gcaacccata
    16861 aattgggtgg tgaaaactat gtcctgtggg gcggtcgtga aggttacgaa acgctgttaa
    16921 ataccgactt gcgtcaggag cgtgaacaat tgggccgctt tatgcaaatg gtggttgagc
    16981 ataaacataa aatcggcttc cagggcacgt tgcttatcga gccgaaaccg caagaaccga
    17041 ctaaacatca atatgattac gatgctgcga cagtctatgg cttcctgaaa cagtttggtc
    17101 tggaaaaaga gattaaactg aacattgaag ctaaccacgc gacgctggca ggtcactctt
    17161 tccatcatga aatagccacc gccattgcgc ttggcctgtt cggttctgtc gacgccaacc
    17221 gtggcgatgc gcaactgggc tgggacaccg accagttccc gaacagtgtg gaagagaatg
    17281 cgctggtgat gtatgaaatt ctcaaagcag gcggtttcac caccggtggt ctgaacttcg
    17341 atgccaaagt acgtcgtcaa agtactgata aatatgatct gttttacggt catatcggcg
    17401 cgatggatac gatggcactg gcgctgaaaa ttgcagcgcg catgattgaa gatggcgagc
    17461 tggataaacg catcgcgcag cgttattccg gctggaatag cgaattgggc cagcaaatcc
    17521 tgaaaggcca aatgtcactg gcagatttag ccaaatatgc tcaggaacat aatttgtctc
    17581 cggtgcatca gagtggtcgc caggagcaac tggaaaatct ggtaaatcat tatctgttcg
    17641 acaaataacg gctaactgtg cagtccgttg gcccggttat cggtagcgat accgggcatt
    17701 tttttaagga acgatcgata tgtatatcgg gatagatctt ggcacctcgg gcgtaaaagt
    17761 tattttgctc aacgagcagg gtgaggtggt tgcttcgcaa acggaaaagc tgaccgtttc
    17821 gcgcccgcat ccactctggt cggaacaaga cccggaacag tggtggcagg caactgatcg
    17881 cgcaatgaaa gctctgggcg atcagcattc tctgcaggac gttaaagcat tgggtattgc
    17941 cggccagatg cacggagcaa ccttactgga tgctcaacaa cgggtattgc gccctgccat
    18001 tttgtggaac gacgggcgct gtgcgcaaga gtgcactttg ctggaagcga gagttccgca
    18061 atcacgagtg attaccggca acctgatgat gcccggattt actgcgccta aattgctatg
    18121 ggttcagcgg catgagccgg agatattccg tcaaatcgac aaagtattat taccgaaaga
    18181 ttacttgcgt ctgcgtatga cgggggagtt tgccagcgat atgtctgacg cagctggcac
    18241 catgtggctg gatgtcgcta agcgtgactg gagtgacgtc atgctgcagg cttgccactt
    18301 atctcgtgac cagatgccag cattatacga aggcagcgaa attactggtg ctttgttacc
    18361 tgaagttgcg aaagcgtggg gtatggcggc ggtgccagtt gtcgcaggcg gtggcgacaa
    18421 tgcagctggt gcggttggtg tgggaatggt tgatgctaat caggcaatgt tatcgctggg
    18481 gacgtcgggg gtctattttg ctgtcagcga aggtttttta agcaagccag aaagcgccgt
    18541 acacagcttt tgccatgcgc taccgcagcg ttggcattta atgtctgtaa tgctgagtgc
    18601 agcgtcatgt ctggattggg ccgcgaaatt aaccggcctg agcaatgtcc cagctttaat
    18661 cgctgcagct caacaggctg atgaaagtgc cgagccagtt tggtttctgc cttatctttc
    18721 cggcgagcgt acaccacaca ataatcccca ggcgaaaggg gttttctttg gtttgactca
    18781 tcaacatggc cccaatgaac tggcgcgagc agtgctggaa ggcgtgggtt atgcgctggc
    18841 agatggcatg gatgtcgtgc atgcctgcgg cattaaaccg caaagtgtta cgttgattgg
    18901 tggcggggcg cgtagtgagt actggcgtca gatgctggcg gatatcagcg gtcagcagct
    18961 cgattaccgt acgggggggg atgtggggcc agcactgggc gcagcaaggc tggcgcagat
    19021 tgcggcgaat ccagagaaat cgctcattga gttgttgccg caactaccgt tagaacagtc
    19081 gcatctacca gatgcgcagc gttatgccgc ttatcagcca cgacgtgaaa cattccgtcg
    19141 cctctatcag caacttctgc cattaatggc gtaaacgtta tcccctgcct gaccgggtgg
    19201 gggataattc acatctatat atctcagcaa ttaattaata tttagtatga atttattctg
    19261 aaaatcattt gttaatggca tttttcagtt ttgtctttcg ttggttactc gtaatgtatc
    19321 gctggtagat atggagatcg ttatgaaaac ctcaaaaact gtggcaaaac tattatttgt
    19381 tgtcggggcg ctggtttatc tggttgggct atggatctca tgcccattgt tgagtggaaa
    19441 aggctatttt tttggcgtgt taatgacagc aacttttggc aactatgcat atcttcgcgc
    19501 agaaaaactc gggcaactgg ataatttttt tacccatatt tgccagttag ttgcgttaat
    19561 cactatcggt ctcttgttta tcggtgtttt aaatgcacct atcaatgctt atgaaatggt
    19621 gatctacccc atcgcctttt ttgtctgctt gtttggtcaa atgcgtttgt ttcgctcggt
    19681 atgagcaaca taaagctctt acacattcag gaatgaaagg aatactgtga tggacaacaa
    19741 aatatcaacc tattcaccgg cctttagtat tgtgtcatgg atagctctcg ttggtggtat
    19801 cgttacctat ctgttagggc tatggaatgc agagatgcag ttaaatgaaa aaggatatta
    19861 ttttgccgta ctggtattag gactgttttc tgcggcgtct tatcaaagga ccgttcggga
    19921 caagtatgaa ggcataccga ccacttccat ttattatatg acctgcctga ctgtctttat
    19981 tatctctgtt gcattactga tggtaggtct gtggaatgcg acattattac tcagcgaaaa
    20041 aggtttttat ggactggctt tcttcttaag cttgtttggt gcagtagcgg tgcagaaaaa
    20101 tattcgtgat gccggaataa acccaccaaa agaaacacag gttacccagg aagaatacag
    20161 cgaataactc acgtaagccc ggtcagtcca atgtgaccgg gcttttactt aactcactaa
    20221 tctgtttctg tcgattcgtt gtaccagcat agaaagtaac aaactcgctg ccaacgtcgc
    20281 gcaaaaaatc caaataatat ccagtattgg ccaattttta agctcaattc cccgggtgcg
    20341 cagtgcatgg ataatcaagg cgtggaatcc gtatataccc aacgaatggc gggagattaa
    20401 gccaagtccg cgaatggtac gcgtatccag cgtgttttta accagagtca atagcgcgat
    20461 tgcgcagata aaaaccatcg gcccacagta aagataccag gtatcggcaa aatttctgcg
    20521 ccactgcaat tcatataatg tcccgcgaga gataataaaa actcccgtcg caaacagtgc
    20581 ggcgctcacc cacgacagtg ctttatgctg tgtgtccatc atccctatag cgcggcccaa
    20641 catgccatac agaatgtagt aaaaagtatc gccattgata tataagttaa ttggcagcca
    20701 ttcaaaaccg tcgattttct gcggcacggt gtttgggtta gcgatgatac caatcaccac
    20761 cattagcacc agcaacattt ttccgccgac gttcttcacc tgaatcagcg gtgaaaccag
    20821 ataaatcacc gcaatcgcga agaaaaacca taagtggtaa aacactggct tttgcagcag
    20881 gtttttcagc gctaactcca cattaatgga ggtaaacagt gcaatgtaga gcagcgcgat
    20941 tgcgctataa aaaaacagac ataagccgat acgcaagaaa tggcgcggct gggcgctgcg
    21001 ttcgccaaaa aagagatagc cggaaatcat gaaaaatagc ggcacgctga cacgagaggc
    21061 agagttcaga acattggcga tatcccaggt gacggggcta acactatgag cattggtcac
    21121 ataccaggta gtggtgtgaa tcatcaccac cattaaacac gctatccctc gcaggttatc
    21181 aatccagtaa attttgggct gcatctgtgt ctctgtatct ggttaaaaaa agtctgaccg
    21241 ataaatcatt cagttggcgc actggaataa tctgagtttt aacactacag cttatagagg
    21301 cttaaggaaa ttcgtaagat atcagccact ataccgatat aaataataag actcacctgc
    21361 aaaccagacg gtaatttaat gatgatgaac gctttctttc cggcaatggc gcttatggtg
    21421 ctagtgggtt gttctacacc gtcacccgtg cagaaagcac aacgggtaaa ggttgatcct
    21481 ctgcgttcgt tgaatatgga agcgttatgc aaggatcagg cggcaaaacg ttataacacc
    21541 ggcgagcaaa aaatcgacgt caccgccttc gaacagttcc agggaagcta tgaaatgcgc
    21601 ggttatacct tccgtaaaga gcagtttgtc tgttcttttg acgcagatgg ccattttttg
    21661 catctttcca tgcgttaagc cctgcttttt cccgtttcat actgtatatc ttccatccag
    21721 cgggtatact gatcccttcc tttaaatcca cacgtatcca gcacgaaata atatgcaaaa
    21781 gtttgatacc aggaccttcc agggcttgat cctgacctta caggattact gggctcgcca
    21841 gggctgcacc attgttcaac cattggacat ggaagtcggc gcgggaacct ctcacccaat
    21901 gacctgtctg cgcgcgctgg ggccagaacc gatggcggct gcttatgttc agccttctcg
    21961 tcgcccgacc gatggtcgct acggcgaaaa ccccaaccgt ttacagcact actatcagtt
    22021 ccaggtggtc attaagccat cgccggacaa tattcaggag ctgtaccttg gttctctgaa
    22081 agagctgggc atggacccga ctatccacga tatccgtttc gtggaagata actgggaaaa
    22141 cccgacgctg ggtgcctggg gactgggctg ggaagtgtgg ctaaacggca tggaagtgac
    22201 gcagttcact tacttccagc aggttggtgg tctggagtgt aaaccggtta ccggcgagat
    22261 cacctacggt ctggaacgtc tggccatgta cattcagggc gtagacagcg tttacgacct
    22321 ggtctggagc gacggcccgc tgggtaaaac cacctacggc gacgtgttcc atcagaacga
    22381 agtggagcag tccacttaca acttcgaata cgcggacgtg gacttcctgt tcacctgctt
    22441 cgagcagtac gagaaagaag cgcagcagct gctggcgctg gaaaatccgc tgccgctgcc
    22501 agcttacgag cgtattctga aagccgccca cagcttcaac ctgctggatg cgcgtaaagc
    22561 catctccgtc accgagcgtc agcgctacat tctgcgcatt cgcaccctga ccaaagcagt
    22621 ggcagaagca tattacgctt cccgtgaagc cctcggcttc ccgatgtgca acaaagataa
    22681 gtaagaggcg gctatgtctg agaaaacttt tctggtggaa atcggcactg aagagctgcc
    22741 accaaaagca ctgcgcagcc tggcagagtc ctttgctgcg aactttactg cggagctgga
    22801 taacgcaggc ctcgcacacg gcaccgttca atggtttgct gctccgcgtc gtctggcgct
    22861 gaaagtagct aacctggcgg aagcgcaacc ggatcgtgaa atcgaaaaac gcggcccggc
    22921 gattgcccag gcgttcgacg ctgaaggcaa accgagcaaa gcggcagaag gttgggcgcg
    22981 tggttgcggt attaccgttg accaggctga gcgtctgact accgataaag gcgaatggct
    23041 gctgtatcgc gcccatgtga agggcgaaag cactgaagca ctgctgccga atatggttgc
    23101 gacttcgctg gcgaaactgc cgattccgaa actgatgcgc tggggtgcaa gcgacgtgca
    23161 cttcgtgcgt ccggtgcaca ccgtgaccct gctgctgggc gacaaagtca ttcctgcaac
    23221 cattctgggc attcagtccg atcgcgtgat tcgcggccac cgctttatgg gcgagccgga
    23281 attcaccatc gataacgccg atcagtatcc ggaaattctg cgtgagcgtg ggaaagtcat
    23341 cgccgattac gaagaacgta aggcgaagat taaagccgat gccgaagaag cagcgcgtaa
    23401 gattggcggt aacgctgact taagcgaaag cctgctggaa gaagtggctt cgctggtgga
    23461 gtggccggtc gttctaaccg cgaaatttga agagaaattc ctcgcggtgc cgtctgaagc
    23521 actggtttac accatgaaag gtgaccagaa gtacttcccg gtgtatgcga acgacggcaa
    23581 actgctgccg aactttatct tcgttgccaa catcgaatcg aaagatccgc agcaaattat
    23641 ctctggtaac gagaaagtcg ttcgtccacg tctggctgat gccgagttct tcttcaacac
    23701 cgaccgtaaa aaacgtctgg aagataacct gccgcgtctg caaaccgtgc tgttccagca
    23761 acagctgggt acgctgcgtg acaagactga ccgcatccag gcgctggctg gctggatcgc
    23821 cgaacagatt ggcgctgacg ttaaccacgc aacccgtgcg ggcctgctgt ctaagtgtga
    23881 cctgatgacc aacatggtct tcgagttcac cgacacccaa ggcgttatgg ggatgcacta
    23941 cgcgcgtcac gatggcgaag cggaagacgt agccgtcgcg ctgaatgagc agtatcagcc
    24001 gcgctttgcc ggtgatgacc tgccgtccaa cccggtagct tgtgcgctgg cgattgctga
    24061 taagatggat actctggcgg gtatcttcgg tatcggtcag catccgaaag gcgacaaaga
    24121 cccgtttgcg ctgcgtcgtg ccgcacttgg cgtgctgcga attatcgttg agaagaacct
    24181 caaccttgat ctgcaaacgc tgaccgaaga agcggtgcgt ctgtatggcg ataagctgac
    24241 taatgccaac gtagttgatg atgttatcga ctttatgctc ggtcgcttcc gcgcctggta
    24301 tcaggacgaa ggttataccg ttgacaccat ccaggcggta ctggcgcgtc gtccgactcg
    24361 tccggctgat ttcgatgccc gcatgaaagc ggtatcgcac ttccgtaccc tggaagcagc
    24421 tgcagcactg gcggcggcga acaaacgtgt atctaacatc ctggcgaaat ctgacgaagt
    24481 gctgagcgat cgcgtgaatg cctctactct gaaagagccg gaagaaatta aactggcgat
    24541 gcaggttgtg gtgctacgtg acaagctgga gccgtacttt gcggaaggtc gttaccagga
    24601 tgcgctggtc gaactggcag agctgcgtga accggtcgat gccttcttcg ataaagtgat
    24661 ggtcatggtt gatgacaaag aattgcgtat caaccgtctg accatgctgg agaaactgcg
    24721 cgaactgttc ctgcgcgttg cggatatttc gctgttgcag taataacgcc gttattaaaa
    24781 agcctgccat ctggcaggct ttttttatcg ctaaataata cagcaacctt taataatctt
    24841 ctgctgaata aagattatct catatattaa ttttatgaga tttttttagg attatatcaa
    24901 ggagaagaaa caaacttatt aagctagaat agccacgggt gcttgagact gtttgtctca
    24961 ggtattcacc gaaaggcaga cagagaaaag ccccacctga ctataaatca aagtgaggcc
    25021 cctctatgca tagacaccat aaggttagcc tcttacttgt tggaaatcaa cacagggggc
    25081 tgggaatgcc gcagaaatat agattgcttt ctttaatagt gatttgtttc acgcttttat
    25141 ttttcacctg gatgataaga gattcactgt gtgaattgca tattaaacag gggagttatg
    25201 agctggcggc atttttagcc tgcaatttga aagagtaaga gtcttcggcg ggaagttatt
    25261 cccgcctttt ttacggcgtt gcgcattctc attgcaccca aatttatttt tcacaaaaat
    25321 aatactagat tttattacgc gatcgattat ttatttcctg aaaaacaaat aaaaaaatcc
    25381 ccgccaaatg gcagggatct tagattctgt gcttttaagc agagattaca ggctggttac
    25441 gttaccagct gccgggcctt tagcgccgct ttcgatggtg aaggacactt tctgaccttc
    25501 gtccagagat ttgtaaccat cgttctggat agcagagaag tgtacgaaca cgtctttaga
    25561 gccatcgtca ggagtgatga agccgaagcc tttgtcagcg ttgaaccatt ttacgatacc
    25621 agtcatttta ccggacatag tgtattacct ttaataatta agtgtgcctt tcggcgatat
    25681 ggcgtgcttt acagattttg aagcgttaaa ggaatgtgca ctacgagggg tatcaacgat
    25741 aactcttgaa gggacttgcc ttactacact gctttaatgg tctgtacgtc aaaccgttga
    25801 tgtgcattaa gccacgcatt ggcgggtgat gcaacaatta tttttcatat ttatgattaa
    25861 tcggttggaa aacggtcctg tcatcaggac cgtaaacagc aataaagtgg ataaaagtct
    25921 attccatcaa ctgcttactt aatgccgggt tggcttgaat caaacgcatc aattttagtt
    25981 cggcacttga aggcttcacg cgtctggatt cccattcctt taccatggcg actgatacgc
    26041 ccaaaacccg ggcgaaatca tcgattttta atcctgtccc ttttcgtaac tgctcaattt
    26101 cggtacagga cgttgttctg tgcgtcaggg taattttctg cgtttcatct ttaaaaacaa
    26161 tctgttccag gctgctcaac agctcatgca ttggatcttt atattccatt gaaaactcct
    26221 caaatcacac tgcgggatcg tgaattgcat cgaagctcat taagaatagt cgggaaaagc
    26281 agacacagcg gggtgaagtc agggcatgct ctggctatga ttcgattcag ccagtcattt
    26341 agcgtgactt atagccacct aacgatctga taaatgccga tatatctctt accactcctg
    26401 gtttgtaaag atttcgaaaa tctaaccttc tgcgaaggcg taaatctctg gctacaaaaa
    26461 aagcaacggc actatcattt tttaacaatg aaacaacaaa ttaaccttgt ttcattttct
    26521 ccatgatttt accaggaaga ttacgtagaa agggtatcct gcaagcctgt cctggactat
    26581 ccttgtcacg tcagacacgc gtgtgtcgtt gtgcgctttt tttgggtgaa aggagtaaga
    26641 aaatggcgac aggaaagtcc tgctctcgct ggtttgcgcc tcttgcggcg ttattaatgg
    26701 tagttagcct gagtgggtgt tttgataaag aaggcgatca gcgtaaagcg tttatcgact
    26761 tcctgcagaa tacagtgatg cgtagcggtg aacgtctacc aaccctgact gccgatcaga
    26821 aaaaacagtt tggtcctttt gtctctgatt acgcgattct gtatggttat tctcagcagg
    26881 tgaatcaggc gatggattcc ggtctgcgcc cggttgtaga cagcgttaac gcaattcgcg
    26941 taccgcagga ttacgttacg caaagcggtc cgctgcgtga aatgaatggt tcgctgggtg
    27001 tgctggcgca acaactgcaa aatgcgaagt tgcaggccga tgctgcgcac tctgcgctaa
    27061 aacagagtga tgacctgaaa ccggtctttg atcaggcatt caccaaagtg gtaacgacgc
    27121 cagctgatgc attgcaaccg ttgattccgg cggcacaaac tttcacgcaa caactggtga
    27181 tggttggaga ctacattgcc caacagggta ctcaggtgag ctttgtagca aatggcatcc
    27241 agttcccgac ttcacagcag gcaagtgaat ataacaaact gattgcgcca ttaccggcac
    27301 agcatcaggc gtttaatcag gcctggacta cagcagttac tgcaacccaa taaagagtaa
    27361 aagcccgagc gattatcggg ctttactcca cgcagtcgcg gcttagtccg cgacgtgcgg
    27421 attcacacag ttcttctcaa cctttccttg taacgcatca atcaaattat ccacggcaca
    27481 ggcggccatg ccataacgcg tctcatgggt ggcagatcca atatgcggta ctgcgacgac
    27541 gttggccatt gagagcaacg gcgaatctac ggaaagtggc tcttgttcga agacatccag
    27601 cccggcggcg tgaatttcgc ctttctgcaa tgcagcaatc agtgcatttt catcaaccac
    27661 cgggccacgt ccggcattaa tgaatatggc ggaggatttc attttggcga attgttctgc
    27721 gccaaataga tgatacgtct catcagttaa cggcaggatc aggcaaacga aatctgactc
    27781 ttgtaacagt gtatccaaat cgcagtagcg ggcgttgaag cgttcttctg cttctttatg
    27841 gtggcggcgc gcgttataga ggatgggcat gttgaagcca aagtgcgcac gttgtgccag
    27901 cgccataccg atccgtccca tcccgacaat gcccagtgtt ttatggtgaa cgtcagtgcc
    27961 gtaccagtcc gggcctatgc tcgcggtcca ttcgcctgct tttacccgtt ctgctacctc
    28021 cacaacccga cgagcggtag acaacatcag cgccatcagc gtatcggcga cggtttctgt
    28081 taatacggtt ggcgtgtgca tgagcagaat ttttcgggcg gtaagcgcat cgacatcaaa
    28141 attgtcatag ccgacggaga tcgttgatgt agcacgcagt ttcggcattt tttccagcaa
    28201 tgcggcatct acattctcgt ttgaacccag taaaccttca gcttctgcaa aaattgctgc
    28261 attttgatcg acggtttgtg ggctgaggtt tgccacctgg tgaacggtga aatgctcttg
    28321 caggcgttgc agtaaatcat caggtaaggc tttgtagagg ataacggacg gcttcatgct
    28381 tctctccatt cactgataag gcgatcacct tagcctgaaa tatgatgaag gaaaactgaa
    28441 gaatgccagg tgatttctgc atcacctggc atgaaaggga ttacagcggg cttaaggtaa
    28501 tttctacacg gcggttttgc gccttacctt ctgcggtgct gttgctggcg attgggttag
    28561 ccgggccaag gccctgagta cggatgcggc tggcgtccac gccctgggtg atcaacgcgc
    28621 tggcaacgga atccgcacgc tgctgggaga gacgcatgtt cagatcgtga ccacccgtgc
    28681 tgtcggtata accaatcacg ttaaccgccg ttttcggata ctctttcagc accattgcca
    28741 cgccggtcag ggtgttagcg cccgcaggtt tcagggtcgc gctgctgctg tcgaaggtca
    28801 cattgttcgg catattgagg ataatgttat ccccgctgcg ggttacacta acaccagtgc
    28861 cgcgcatttt gtcacgcagc ttcgcttcct gcacatccat gtaataaccc acgccgccgc
    28921 ccagagctgc gcctgctgct gcgccaatca gcgcgccttt accgcgatct ttcttcgaag
    28981 aagagagcgc accaataccc gcgcccacga gagagcccag acctgcgccg atagcagatt
    29041 tacctgcttc gcgttcgccg gtgtaagggt tagttgtgca gccagatacc gccagagcgc
    29101 cactcactac ggcggcaata agataaacac gtttcttcat tgttaatcct taataacctt
    29161 tttattcttt gccacgggtt ccgtggcggg agattatgcc gcgtgaacat gaagattatt
    29221 cctgggaata ctcggaaatt tgtaagtaat atttaactgc tcaatacatc taacctttca
    29281 ggagtcttcg gtttggccaa ctcatcctca cgatattccg ttctgactgc cgcccattgg
    29341 gggcccatgc tggttgaaac cgacggcgaa accgtgttta gctcgcgtgg cgcgttagcc
    29401 acaggaatgg aaaactcctt gcagagcgcg gttcgcgacc aggttcacag caatacgcgg
    29461 gtacgatttc caatggtgcg caaaggcttt ctcgcgtcac cggaaaaccc gcaaggcatt
    29521 cgtgggcagg atgaatttgt tcgcgtgagt tgggatgagg cgctggatct tattcaccaa
    29581 caacataaac gcattcgtga ggcttatggt ccggcatcga tttttgctgg ttcctacggc
    29641 tggcgttcaa acggcgtgct gcataaggcc tcgacattat tacaacgcta tatggcgctg
    29701 gcaggcggtt ataccgggca tctgggggat tattcgaccg gtgcggcaca ggcgatcatg
    29761 ccgtatgtcg tgggtggcag cgaagtttat caacagcaaa ccagttggcc gctggtgctg
    29821 gaacatagcg atgtcgtggt gctgtggagt gctaacccac tcaatacgct gaaaattgcg
    29881 tggaatgcat ctgatgagca ggggctttct tacttttccg cactgcgtga cagcgggaaa
    29941 aagctgatct gcattgatcc aatgcgatcg gaaaccgtcg atttctttgg cgataaaatg
    30001 gagtgggtgg caccgcacat gggcaccgat gttgcgctga tgctggggat cgcccatacg
    30061 ttggtggaaa atggttagca cgacgaagcg tttctggcgc gttgcaccac cggttatgcc
    30121 gtcttcgcct cttatttgct gggcgagagt gacggaatag cgaaaaacgc cgaatgggcg
    30181 gcagagattt gtggtgttgg cgcagcgaaa atccgcgagc tggcggctat tttccaccaa
    30241 aataccacca tgctgatggc aggctgggga atgcagcgcc aacagtttgg tgagcaaaaa
    30301 cactggatga tcgtcacgct ggcagcaatg ttggggcaaa tcggcacacc cggcggcggt
    30361 tttggtcttt cttaccattt tgccaatggt ggtaacccca cgcggcgttc tgcggtgctc
    30421 tcttccatgc agggcagctt gccgggtggc tgcgatgcgg tagagaaaat ccctgtggcc
    30481 cgcattgttg aagcactgga aaaaccaggt ggcgcatatc aacacaacgg tatggaccga
    30541 cattttccgg atattcgttt tatctggtgg gcgggcggtg ccaactttac tcatcatcag
    30601 gataccaacc gcctgatccg tgcctggcaa aaaccggagc tggtggtgat ctccgaatgt
    30661 ttctggacgg cggcggcgaa acacgcggat atcgttctgc ctgcgactac ctcttttgag
    30721 cgtaatgatc tcaccatgac cggtgattac agtaatcagc atctggtgcc gatgaagcaa
    30781 gtggtgccgc cacgctatga agcgcgtaat gattttgatg tttttgccga gttaagtgaa
    30841 cgctgggaga agggcggcta tgcgcggttt acggaaggaa aaagtgagct gcaatggctg
    30901 gaaatgtttt ataacgttgc ccgacagcgc ggggcaagcc agcaggttga attgccgcca
    30961 tttgctgagt tctggcaagc caatcagtta attgagatgc cggaaaaccc ggacagcgag
    31021 cggtttattc gcttcgctga tttttgccgc gatccgcagg cgcatccatt aaaaaccgcc
    31081 agcggtaaga ttgaaatctt ctcgcagcgt attgccgatt acgcttatcc ggattgcccg
    31141 gggcatccaa tgtggctgga gccggacgaa tggcagggta atgccgaacc ggaacagtta
    31201 caggtacttt ctgctcatcc ggcgcatcgt ctgcacagcc agctgaatta cagttctctg
    31261 cgcgaattgt acgcggtagc aaaccgtgag cctgtgacca ttcatcctga cgatgctcag
    31321 gcgcgcggca tacaagatgg cgatactgtt cggctgtgga actcacgcgg gcaaattctt
    31381 gccggagcgg tcattagtga gggaattaaa cctggcgtga tttgcattca cgaaggggca
    31441 tggccggatc tggatttaac cgctgacggt atttgtaaaa acggcgcagt gaacgtgctg
    31501 accaaagatc tccccagctc gcggctgggg aatggctgtg cgggtaatac ggcgctggca
    31561 tggctagaaa aatacaacgg tccggaactg acacttacag cgtttgaacc accggccagc
    31621 tcataatcca ggtgggtagt tgggtttcat cctgccatgc gcaatcgaca atgtgaaaac
    31681 cctgcgcctg gtaaaaatct atcgccggtt gatttttttg ataaacctcc agcatcagat
    31741 ggggatggcg ctgttgcaca tactgcatca gcgccttacc aataccgcgc ctgacggcct
    31801 tcggtgcgac aaacatcgct gccagaaatc ggccttccat aatgctgaca aaaccaagaa
    31861 gcttactgtc ttcttcccag acccagtttt gtgcgttggc aagataggca tcccgcacca
    31921 gcggaatgca ctcgcgccag taattcgctt ttataaaggg atggccccag gttgtacttt
    31981 ccagccacag ttcgaggatc gcggggagtt ctgaacgttg cgcttcccta atcatggttt
    32041 atttcccgga tagcaacagc agccaaccac atgatcattc accagcccac atgcctgcat
    32101 aaaggagtaa cagattgtgg taccgacaaa cttaaaacca cgttttttca gtgccttaga
    32161 tagggcgtcg gaggcgggcg tagatgtggg gatttcgctc aacgttgtgg cttgtgtcac
    32221 ctgtggctga tgatttacaa acgaccagac aaagtcggca aacggttcgc cgttctgttc
    32281 catttgcagg tacgcccgcg cattaccaat aattgcctga attttcccgc gatggcggat
    32341 aatcccggcg tcctgtacca gtctttcgat atcctcttcc tgcattgcgg caaccttcac
    32401 cggatcgaac tgatgaaagc aggcgcgata gttttcgcgt tttttgagga cggtgatcca
    32461 cgataaccca gcctgctgcc cttccaggca gatcatttcg aacagttttt tactgtcagt
    32521 ttcaggcacg ccccactcat tatcatggta ggcaatataa agcgggtcct gactcaccca
    32581 gccgcaacgt tccatacttt ccctcgctat gtacccgatc gtaaaaattt cagccgcttt
    32641 tcttgacgca tcggtgaaaa agtcaatatt taatacaggg ctactatgcc cgatatcctt
    32701 catacaatga caataatatt gccgaattcg gctcttccct ggagtcgtgt taatgatcat
    32761 aaaaaaaagc ggtggtcgct ggcagctaag cctgctggcg agtgtggtaa tcagtgcctt
    32821 ttttctcaac acagcttacg cctggcaaca agaatatatc gttgatacgc aacccgggca
    32881 ttccacagag cgttacacct gggatagtga tcatcaacct gattacaacg atattttgtc
    32941 gcaacgtatt caaagtagcc aaagggcgct gggactggaa gtcaatctgg cggaagaaac
    33001 ccctgtggat gtgaccagca gtatgagtat gggctggaat tttcctttat atgaacaggt
    33061 tacaactggc ccggtcgcgg cattacatta cgatggcaca accacctcga tgtataacga
    33121 gtttggcgac agtactacca cgctgaccga tccgttatgg catgccagcg tgagtacctt
    33181 aggctggcgt gttgactccc ggcttggcga tctccgaccc tgggcgcaaa tcagctataa
    33241 ccagcaattt ggcgagaata tctggaaggc gcaatcaggc ctgagccgga tgacggcgac
    33301 aaaccagaac ggcagctggc tggatgtcac cgtaggcgct gatatgttgc tcaatcaaaa
    33361 tattgccgcc tatgccgcgc tatctcaggc agaaaatacc actaacaata gcgattatct
    33421 gtatacgatg ggggttagtg ccagatttta acgtaacaat cacaattgaa accattgaat
    33481 aacaatagtt gtggcgatag tgggtgctaa cttaccaaat aataaatttg gtgaataatt
    33541 gtcgcgtcat tcattcctga actaaggcat ttcattccgt tctgatggca tttcatgccg
    33601 tttttcccca ggcataaagt gcacttcgtt atggttgtcg gcagagattt ttccttttta
    33661 ttactgcagg aatactgcca tgacaccttc aaattatcag cgtacccgct ggctgacact
    33721 catcggtact attattaccc agtttgcgct ggggtcggtt tatacctgga gcctgtttaa
    33781 cggcgcgctt tccgccaagc tggatgcgcc ggtaagccag gtcgctttct ctttcggctt
    33841 gttaagtctg gggctggcaa tttcgtcttc tgttgcgggc aaattgcagg aacgttttgg
    33901 tgttaaacgc gtcaccatcg cttccggcat tttgctggga ttagggttct tcctgacagc
    33961 gcattctgac aacctgatga tgctgtggtt aagcgccggt gtgctggtcg gtctggcgga
    34021 tggcgcgggt tacctgctga cgctctctaa ctgcgtgaaa tggttcccgg agcgtaaagg
    34081 tctgatctcc gcgttcgcta tcggttctta tggtctgggt agcctgggtt tcaaatttat
    34141 cgacacgcag ctgctggaaa cggtcggtct ggaaaaaacc tttgtgattt ggggagcgat
    34201 tgcgctggtg atgattgttt tcggcgcaac gttaatgaaa gacgcaccaa aacaggaagt
    34261 gaaaaccagc aatggtgtgg tggagaaaga ttacacgctg gcagagtcga tgcgtaaacc
    34321 gcagtactgg atgttagcgg taatgttcct gactgcctgc atgagcggcc tgtacgtgat
    34381 tggggtagcg aaagatatcg cccaaagtct ggcacacctt gatgtggttt ctgcagccaa
    34441 tgcagtcact gttatttcca tcgccaacct ttcaggtcgt ctggtgctgg gcattctgtc
    34501 tgacaaaatc gcccgtatcc gtgttatcac cattggtcag gtgatttcgc tggtgggtat
    34561 ggcggccctg ctgtttgcac cattgaatgc agtgacgttc tttgcagcga ttgcctgtgt
    34621 ggcatttaac tttggcggca ctattaccgt ctttccgtca ctggtcagtg agttcttcgg
    34681 cctcaataac ctggcgaaaa actacggtgt gatttacctc ggtttcggta tcggtagcat
    34741 ttgtggttcg attatcgcct cactgtttgg cggcttctat gtgacgttct acgtcatctt
    34801 tgctctgctg attctgtcat tggcgctttc tacgacgatt cgtcagccag agcagaaagt
    34861 attgcgtgag gcgcatattt agccagaaac tacagctttt gaacgaaagg gggaggaaat
    34921 cctccttttt tattgcattt taaagaataa aaatgaaaat attttatctt attatttgtg
    34981 tggtgaatgt ttttgtttt
//
LOCUS       DAZFFG010000036        34392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFFG010000036 DAZFFG010000000
VERSION     DAZFFG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.036"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            26..3208
                     /locus_tag="ACHJ7J_002150"
                     /pseudo
     CDS             26..3208
                     /locus_tag="ACHJ7J_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            6101..6961
                     /gene="pdxI"
                     /locus_tag="ACHJ7J_002151"
     CDS             6101..6961
                     /gene="pdxI"
                     /locus_tag="ACHJ7J_002151"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO5000385.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDILDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            7025..9331
                     /gene="ydbD"
                     /locus_tag="ACHJ7J_002152"
     CDS             7025..9331
                     /gene="ydbD"
                     /locus_tag="ACHJ7J_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO5000386.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALKIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIASMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLERALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVFKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            9501..10106
                     /gene="ynbA"
                     /locus_tag="ACHJ7J_002153"
     CDS             9501..10106
                     /gene="ynbA"
                     /locus_tag="ACHJ7J_002153"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5000387.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNLQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFFTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWGIASILLIWTAINRCRSVLLMSAER"
     gene            10106..11002
                     /gene="ynbB"
                     /locus_tag="ACHJ7J_002154"
     CDS             10106..11002
                     /gene="ynbB"
                     /locus_tag="ACHJ7J_002154"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5000388.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            11018..12775
                     /gene="ynbC"
                     /locus_tag="ACHJ7J_002155"
     CDS             11018..12775
                     /gene="ynbC"
                     /locus_tag="ACHJ7J_002155"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO5000389.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLLLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLNELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            12789..14081
                     /gene="ynbD"
                     /locus_tag="ACHJ7J_002156"
     CDS             12789..14081
                     /gene="ynbD"
                     /locus_tag="ACHJ7J_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO5000390.1"
                     /translation="MLQGAGWLLLLTPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVSWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIKIAQPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(14132..14737)
                     /gene="azoR"
                     /locus_tag="ACHJ7J_002157"
     CDS             complement(14132..14737)
                     /gene="azoR"
                     /locus_tag="ACHJ7J_002157"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO5000391.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            14938..18840
                     /gene="hrpA"
                     /locus_tag="ACHJ7J_002158"
     CDS             14938..18840
                     /gene="hrpA"
                     /locus_tag="ACHJ7J_002158"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO5000392.1"
                     /translation="MTEQQKLIFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARSEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPIDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAIDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            19112..19912
                     /gene="ydcF"
                     /locus_tag="ACHJ7J_002159"
     CDS             19112..19912
                     /gene="ydcF"
                     /locus_tag="ACHJ7J_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO5000393.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            20109..21548
                     /gene="aldA"
                     /locus_tag="ACHJ7J_002160"
     CDS             20109..21548
                     /gene="aldA"
                     /locus_tag="ACHJ7J_002160"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO5000394.1"
                     /translation="MSVPVQHPMYFDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMYEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(21590..22591)
                     /gene="gap"
                     /locus_tag="ACHJ7J_002161"
     CDS             complement(21590..22591)
                     /gene="gap"
                     /locus_tag="ACHJ7J_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5000395.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            22780..23310
                     /gene="cybB"
                     /locus_tag="ACHJ7J_002162"
     CDS             22780..23310
                     /gene="cybB"
                     /locus_tag="ACHJ7J_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO5000396.1"
                     /translation="MENKYSRLQIGIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            23555..23728
                     /locus_tag="ACHJ7J_002163"
     CDS             23555..23728
                     /locus_tag="ACHJ7J_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000397.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            23754..23822
                     /gene="yncP"
                     /locus_tag="ACHJ7J_002164"
     CDS             23754..23822
                     /gene="yncP"
                     /locus_tag="ACHJ7J_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO5000398.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(23800..23949)
                     /gene="hokB"
                     /locus_tag="ACHJ7J_002165"
     CDS             complement(23800..23949)
                     /gene="hokB"
                     /locus_tag="ACHJ7J_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO5000399.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            24348..25988
                     /gene="trg"
                     /locus_tag="ACHJ7J_002166"
     CDS             24348..25988
                     /gene="trg"
                     /locus_tag="ACHJ7J_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO5000400.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMMKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTSLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEQVSFATV"
     gene            complement(26026..26949)
                     /gene="ydcI"
                     /locus_tag="ACHJ7J_002167"
     CDS             complement(26026..26949)
                     /gene="ydcI"
                     /locus_tag="ACHJ7J_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5000401.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLNRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            27166..28509
                     /gene="ydcJ"
                     /locus_tag="ACHJ7J_002168"
     CDS             27166..28509
                     /gene="ydcJ"
                     /locus_tag="ACHJ7J_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5000402.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDDETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            28734..30389
                     /gene="opgD"
                     /locus_tag="ACHJ7J_002169"
                     /gene_synonym="mdoD"
     CDS             28734..30389
                     /gene="opgD"
                     /locus_tag="ACHJ7J_002169"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO5000403.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDTEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            30529..30753
                     /gene="ydcH"
                     /locus_tag="ACHJ7J_002170"
     CDS             30529..30753
                     /gene="ydcH"
                     /locus_tag="ACHJ7J_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO5000404.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESIKEV"
     gene            30816..31355
                     /gene="rimL"
                     /locus_tag="ACHJ7J_002171"
     CDS             30816..31355
                     /gene="rimL"
                     /locus_tag="ACHJ7J_002171"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO5000405.1"
                     /translation="MTETIKVSESIELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(31347..32327)
                     /gene="ydcK"
                     /locus_tag="ACHJ7J_002172"
     CDS             complement(31347..32327)
                     /gene="ydcK"
                     /locus_tag="ACHJ7J_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO5000406.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHESLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGKSR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            32451..33443
                     /gene="tehA"
                     /locus_tag="ACHJ7J_002173"
     CDS             32451..33443
                     /gene="tehA"
                     /locus_tag="ACHJ7J_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO5000407.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            33440..34033
                     /gene="tehB"
                     /locus_tag="ACHJ7J_002174"
     CDS             33440..34033
                     /gene="tehB"
                     /locus_tag="ACHJ7J_002174"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO5000408.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKERELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         8716 a         7947 c         9020 g         8709 t
ORIGIN      
        1 acggtctaga tctaaggaat tttatatgca aaggaaaaaa ttattgtctg tttgtgttgc
       61 catggcgtta agtagccaga catgggctgc ggatacttca atacccgact caaccgaaaa
      121 aacacgtaag tccagtaaga taacatgtcc ggcaaatatt cattccctga gcaaagagca
      181 gctagaaaat ttatccgcgg agtgcagaga aaataaagat agtgcagttt taccatgggc
      241 agctgcaggt cttgctgcag tagctacggg aattgctata tataccttaa gtgacgatga
      301 caatcaccac cataataact ccccagttcc ggatgatggc ggtgatacgc ctgttccgcc
      361 agacgacggc ggtgatacgc cggttccgcc agatgacggc ggtgatacgc cagttccgcc
      421 agatgacggc ggtgatacgc cagttccgcc agatgacggc gatgatacgc cggttccgcc
      481 agatgacggc ggtgatacgc cagttccgcc agatgacggc ggtgatacgc cagttccgcc
      541 agatgacggc ggtgatacgc cggttccacc agatgacggc ggtgatacgc cggttccacc
      601 agatgatggc ggtgatacgc cagttccaga cgacggcggt gatacgcctg ttccgccaga
      661 cgatggtggc gataccccgg ttaaacacga tcctgtcgtc tataaaaatg acgtaacctg
      721 ggatcaggac gcgaaaaccg tcaaaattcg cgaaaccacc tttacctaca gccaaaatgc
      781 cgatgggagt tacaccttaa ccgcgcctga tggcctgacg acgatagtca agggctggac
      841 agtccatgac gacagcaaca ccgtcatctt tgatggcgta aatacggcag gtggcatcac
      901 ctggagctac gacgatgatg ggctcatcca tatcactaaa gaggccggtg tcgttgttga
      961 tggcacaacc ggaactgcga ttgattttgg taatgcgatc atcaccgatc agggcggcaa
     1021 tacggcgctg aatggcggta ctgtcatgac ggttgagggt gacaacatca ccctcaacaa
     1081 cgacggtaaa acgaccgcca tcggcgaggg ttcggtcgtt ggtatcctta ctggtgacga
     1141 tatcactatc aataacaacg gcgagacgga agttgacggc ggtaccgcag taatcatcaa
     1201 tggcgataat gctacgctga acactgcggg cgactcgacc atcaccaatg gtggtacggg
     1261 tagccttatc aatggcgata atgcgcgggt cgataatcaa ggtaccatga gcgttgacgg
     1321 tgagaattcc actggctcga aaattgtcgg tgacggtgcc accatcaaac aggaaggcga
     1381 cttatacgta agcggtggtg cgcacggtat tgacgttgac ggcaatgata cgttcgttag
     1441 caacaaaggt aatattacgg tcatcgaaga taactctatt ggcatgctgc ttgatggtga
     1501 tggcgtcagc gtgatcaaca tgggcgacct caatgttggt caggccgctg cgggtgaaaa
     1561 tgcaatcggc attcagatcg acggtgataa tgcgacattt gttaacgttg gtgatatttc
     1621 cgcgaccaat gccggaaccg gtgtcagcgt tgctggggat aaagctaata tttcactcgc
     1681 gggtgggtta gacgttgggg atttctccac cggtcttgat gtctctggca ataataacaa
     1741 catgacgctg gcgacttatg agctgaatgt gacagggcaa aaagcgacgg gggttaatgt
     1801 ttcgggtgat ggcaatacga ttgaaatcgc aggcagcatt ctggttgata aagatcagaa
     1861 agccgataat gcgcaacctt acttctttaa tccctcaacc ggcgtaaata tcagcggtaa
     1921 taacaatgac gtcacgctgg atggtcagtt aaccgttgtt gctgatagcg aaaccacggt
     1981 gcgttcctat gcaagttacg acggcgctca ggagcacatt gcaggtattg ttattgctgg
     2041 cgatgataat acctttacgc ttaatggcgg tgtccatttt gtcggcgaaa aaaatgtcat
     2101 ggatgatggc tcaaaaccat ctgcttctcg tcgtggtatc ggagacacac cgcttattag
     2161 cgttgatggg cattcatcgg tctacctcaa tggtgagtca accattagcg gcgagttccc
     2221 gttgggtttc gaaaatctca ttcagttgag tcatggcgcg gagctggaga ttggcgctga
     2281 cgcgacgttt gatatgagtg atgtggattc cttcacctat tattatcgcg tggcgttatc
     2341 caccattagt attgattctg gtgcgaaggc gaccaataac ggtgaagttg aactgaaaaa
     2401 tatcggtttt gcggctgcct ggaataaaga ttcaacggtc atcaataacg gatccattgg
     2461 cctggcgatg tacgattttg gtaccgaccc ggcaccaaaa gtcttcgatg ttgaatacgg
     2521 ggggattggc gttaacaatg gcaccttgac caccaaaatg atgaatcagc acagcgtgct
     2581 gaattatccg gcagagtgga acttgtctga cggtacctct ttcaataaca gagcattagg
     2641 tttgacaggt atgcttgcca gctactcgtc atccattctg aacggcgaaa cgggcattat
     2701 tgatatgtat ggccgtggca gtgtagggat gctagccatc gataaatcaa ccgcggataa
     2761 cgaagggcaa atcaccctgg atacgctgtg ggttgatgaa aatgatgaaa catcgctgcg
     2821 taataatgtt gcaaatagca cggcgaaaga tttcggtgtg ggaatggctt caggtaccga
     2881 tgcctataat ggggcattaa ctaaagccac ggcaaccaac cacgagaatg gtgtgattac
     2941 tgtctacaac gccggtgcgg ggatggcggc gtatgggaat actaatacgg tcattaacca
     3001 gggaaccatc aacctggaga aaaatgaaaa ttacaacgat tctctcggtg tgaataaact
     3061 cgtcggcatg gcggtttatc acgaaggcac cgccattaat gaccagacag gcgtaatcaa
     3121 cattaacgcg gaaaatggtc aggcattcta taacgatggc actgggctta ttatcaacta
     3181 tggcacaatt tgtacctttg gcgtttgcca gggggccgat gaatataatc caacggataa
     3241 ttatattgcc gatttatatg atgatggcga tgtattaagt tctgcgggtg agaccgtatt
     3301 attatcgtca ggtgcgatta tcaacgggga tgtcaccagt gcgggtgctg ttaaaaatgg
     3361 aacgatcagg atactcgaaa atggtgcgct ggttaacgag tcgagcggaa ccattagctc
     3421 agccatcgtc gtgaatgaag gcggtgcgtt aaccaatgaa ggtgtattga acacggtgac
     3481 tctggaagga ggaaccttca ataacaacgg aacgcttaat gacgtcgtaa aaattgagaa
     3541 aaacagcaac gcggtaatta ataacaccgg ttccctgtca actttacaac ttcacgatgg
     3601 tacggtgaat aacagcggta ttgcgtcggc gcgcgttaac gctcagggcg atgcggtatt
     3661 caataacctt gcaggcggcg aggcgcgtaa aggtgccatt ctctataact ctgcggtggt
     3721 gaataacgcg gggacctgga aaatgggtta tcaggatgaa aataacaatg ccgggacgct
     3781 ggatattgac gaccaatcaa cgttcaacaa cagcggcaag ctcatccttg ataacagtaa
     3841 aaacgccatt cgcttccagg gaagcaatgc taacgccacg ttatataaca ccggtgaaat
     3901 gacgctggat gccgcacttg gcgcgggcgc tattcactat gacgacggcg ccagccagtt
     3961 tattaacaag ggcgtggtgg atgcgaaagt cactgtggcg gtaagtaccg ctggtgcgac
     4021 agaaagcgat gccttcctgt ggaaccagga tggcggggtg attaacttcg ataaagacaa
     4081 cgccagcgca gttaaattca cccacaacaa ctatgttgcc ctcaacgatg gcgtgatgaa
     4141 catcagcggc aacaatgccg tggcgatgga aggtgataaa aacgcacaac tggttaacaa
     4201 cggcgttatc aatctcggca ccgaaggtac caccgatacc gggttaattg gtatgcaact
     4261 ggatgccaac gctaccgcag acgcggtgat cgagaacaac ggcacgatta atatctatgc
     4321 taacgattcc tttgcattca gtgtgttggg aacacaaggc catatcgtta acaacggtac
     4381 cgtggtgatt gccgatggcg tgaccggttc cgggttgatt aagcagggcg acagcgtgaa
     4441 cgtggaaggg gtgaacggca acaacggtaa taataccgaa gtgcattaca ccgactacac
     4501 gttgccggaa gtgccgaaca ccagttctgg tggcgtaaca acagggggag atgcgactgg
     4561 cagcggtagc aacaacctca acggttatgt ggtcggtacc aacgttgacg gcagcgccgg
     4621 taagctcaag gtcaacaacg ccagcatgaa cggtgtcggg atcaacaccg gtttcgccgc
     4681 cggtacggca gacaccacgg tcagtttcga caacgtggtg gaaggcagca acctgaccga
     4741 cgccgatgcc atcacctcaa cgtccgtggt gtggaccgcc aaaggcagca ccgatgccag
     4801 cggcaacgtt gacgtcacca tgagcaaaaa cgcctacacc gatgtggcga ccgatgcttc
     4861 ggtgaacgat gtggcgaagg cactggatgc gggttacacc aacaacgagc tgtataccag
     4921 cctgaacgtc ggcactactg ccgaactgaa cagcgccctg aagcaggtga gcggcagcca
     4981 ggcaaccacg gtattccgcg aggcgcgcgt gctgagcaac cgcttcagca tgctggcgga
     5041 cgccgcaccg aagatgggca acggtctggc gtttaacgtg gtggcgaaag gcgacccgcg
     5101 tgctgaactc ggaaataaca ccgagtacga catgctggcg ttgcgtaaaa ccgttgacct
     5161 gagcgaaagc cagagcctga gtctggagta cggtatcgcc cgtctggatg gcgacggtgc
     5221 gcagaaagcg ggcgacaacg gcgtaaccgg tggttacagc cagttcttcg gcctgaagca
     5281 ccagatgttc ttcgacaacg ggatgaactg gaacaacgcc ctgcgctatg acgtgcataa
     5341 cctcgacagc agccgctcgg tggcttacgg cgacgtcagc aaaacggcag acacggatgt
     5401 gaaacagcag tacctggagt tccgcagcga aggggcaaaa acctttgaac cgcgcgaagg
     5461 gctgaaaatc atcccgtacg ccggagtgaa actgcgtcac acgctggaag gcggctatca
     5521 ggagcgcaat gccggagact ttaacctgag catgaacagc ggcagcgaaa cggcggtgga
     5581 cagcatcgtc gggctgaaac tggactacgc agggaaaggc ggctggagcg cgaatgcgac
     5641 gctggaaggc gggccgaacc tgagctacag caagagccag cgcacggcaa gccttgcagg
     5701 ggcaggcagc cagcacttta acgtcgatga cggtcagaag ggcggcggta tcaacagcct
     5761 ggcgagcgtc ggcgtgaagt acagtagcaa agaaagttcg ctgaatctgg atgcgtatca
     5821 ctggaaagag gacggcatca gcgacaaagg cgtgatgctg aactttaaga aaacgttcta
     5881 atttttagca tgtgatacac aaaccgcaac gctgatacag gttgcggttt tttattgccg
     5941 gatgcgacgc ttgacttatc ttttgctgtc taatgttttg gtgtctttca ggacaatagt
     6001 gctactcatc cagaaatctt gtttacgcct tcgcattatt tatctctttt cgtttctata
     6061 ctgatttttc ttaatccgtt ttattacagg gcagggtgcg atgagcagca atacatttac
     6121 tctcggtaca aaatccgtta accgtcttgg ttatggcgcg atgcaactgg caggtcctgg
     6181 ggtttttggc cccccaagag atcgccacgt cgctataacc gtgctgcgtg aggcgctggc
     6241 attgggcgtc aatcacattg ataccagcga cttttatggt ccgcacgtca ccaatcagat
     6301 tatccgcgaa gcgctttatc cttactctga cgatctgaca attgtcacta aaattggtgc
     6361 gcggcgtgga gaggacgcat cctggttgcc cgcattttct ccggcagagc tgcaaaaagc
     6421 ggtacacgat aatctacgta atctcggcct ggacatactg gatgtggtta acctgcgcgt
     6481 gatgatgggg gatggtcatg gcccggcgga aggatcgatt gaagccagcc tgaccgtgct
     6541 ggcagagatg caacaacaag gcctggtaaa acatattggc ctgagcaacg tcacaccgac
     6601 gcaggttgca gaggcgcgca agattgccga aatcgtctgt gtgcagaacg aatacaacat
     6661 tgctcatcgt gctgatgacg caatgattga agctttggct cgtgacggca ttgcctacgt
     6721 gccgttcttt ccgctcgggg gctttacgcc gctgcaatct tccacgcttt ccgatgttgc
     6781 tgcgagcctg ggtgcaacac caatgcaggt ggcgctggcg tggctgttac aacgttcacc
     6841 gaatattttg ctgatcccag ggacgtcttc tgttgcgcat ttacgggaga atatggctgc
     6901 tgaaaaattg catctttctg aggaagtgtt gtctacgttg gatggtattt cgcgagaata
     6961 acgaatatac aaaagggaaa gatgcatttc ccttttgttt cttttttaat ggcatggagt
     7021 gcatatgttt aagcccagga tttgcagctg gataggttta ctccccctgt ttatgctgag
     7081 tcttccagta caggcggaac ttcgatgtgt tgcaaatgca gttgatattg aaccattttt
     7141 ttctgcggct accgctgaag ataaacaaca agttgaacaa gctatcaaca gtagcgtgaa
     7201 tcttgtcccc ttcggtttat ctgcatcgga ctggaaagtt catcgtggcg atttagtggt
     7261 agaaggtaat atagagagta atcaaaaatt gattgttctt ggcaatttga cagtcaaagg
     7321 taatatttcc actttctctc tttctaatcc atgggttatt ctcggtaacg tgacggcaac
     7381 gaatatagtt acggattcac cactgttaat taccggttcg ataaatgcaa gtggactggt
     7441 atttatcgac tcatattacg ataatccgtc tacgattaag gggagtatta atgcgcgtgg
     7501 gatatttatc aatgacataa ttgcacctgt tgttgcgtct tcgacaaata gtgaattcat
     7561 ggtccgtgcg agtgataaaa atgacactga aaatgtcaaa aaagcgttga agataataaa
     7621 tcctgatgca tattattggg gtctaataaa tgatgaagat gctctgaaag aaatttttaa
     7681 gcgaagcaat attcgcatgg cagggaatgt ctgtaatcag atgaaaaaag aagcgctgtt
     7741 tcgccctaag ccttctcctg agttagtgca ggaattgcaa atgctggatg aaggcaacgt
     7801 tgctgcattt gaaggacgag atattgcgac atttgatctt gccataatgc gaactctccc
     7861 aaggcttaaa ggaatttcag ctaacctacg caaacaactt attaatagca atgatggaca
     7921 aacgattgca agtatggcga gatatatgcc tgacaatgag attctggagt tgaccgatca
     7981 gcaattgggt taccagcctg ttgttctggg gttgctcgac cgtgaaccgc tctctgtcga
     8041 aataatgacg cgaatgagtc atctacctga tggtgtcggt ccgttgaatc ttgcgctacg
     8101 tgaaaatctc cctctggata tcgtcatgac tctggcgaaa agagattggg atatgatcat
     8161 tcaggagctt tataaagatg cgtggttatt gcccgaatcc attattgatg gctatatccg
     8221 cagtgatgat tcctctattc gtcaggtcgg tgctggagga caactcacct acaatcaggc
     8281 aatgcagttg gcgaacgatt catcaaacga tgttgtcaca agcttagcgt taaagctggc
     8341 agagatgaaa caccatggtc aattgttgcg gatgacgcca caagagagtg acaaaatagc
     8401 agtatattta taccaaaaat tcgagaatga cgatgatcta ataggtgcgt tatttttagc
     8461 gttgccagat aacttacagt ttaattttgt taaaaggatg gagaaaaaat cccctgccta
     8521 cttttgctgt cgggatatgc agataattca ctctgacgct gctttacaac ggttattgac
     8581 acgtttcaac gatcctgagg ggtggagtaa tctggcgaaa aatcagtatc tgagcacgtc
     8641 gatgaaacaa aaaatttggc aacgtgcttt atcgcatcgg aaaaataatc ccaaagcaga
     8701 ttcagatgca tatgaaacca gcgctgatat gattttgtct gagcttatca gctacggtga
     8761 agttgatgac cagatgctat taaatgccac ctctctgata cgttcggacg actgggactt
     8821 tttagagaga gcattaatta gttgggataa tttacccgct gttgttctca aggaattaca
     8881 gcaaaacacg ccacgcaatg atatttgggc gaagtttttt ctgaggcagg aaaacagttc
     8941 ccgtgcgcag gttaacgaag cgttacgtgt ttattatgca ctagaccccg atgcgttagc
     9001 acaactggat gtactggcaa aacaaccgga tcgtatatgg tggagtacac tggcgaaaag
     9061 caatctcaca tttttcaagt tcggcgcact taacaaccgc cacacaccgc ctgcagtact
     9121 ggcggcagaa attgatcccg agtggtggat tgtggcgatg aataatcccc gttttccagt
     9181 tgatgtattt aaggcgaggc tgaaacgtga tcctttgctg gcgttagagc ttgttaaccc
     9241 tgaactggat ttagttcgcc agctggcgct taacggtaag acgcgcgcaa tacgggaaca
     9301 agcgatgaga aaacttgatg agttgtattg aataacagcg taataatttt taaccacttg
     9361 atttattggg ataatcattc ctgacagtga gtccccaata ccttgatata ttctgaattt
     9421 ttaatgaagc gtcgtgttgc gatatctccg tcaggggaat tgatgcacca tagcgcaaac
     9481 cgaattatca aggattgata atgacgctct accagataaa accgctcttt cagtcgctgt
     9541 taaggccgac gatgttttgg ctttataagc accatgttac agcgaatcac atcactcttg
     9601 ctgcactggc gctttctctt attaccggat tgctgttgat gttggtcgca caacccatcc
     9661 tctttttgct attgcccatc gtgcttttta tccgtatggc actcaatgcg ctggatggca
     9721 tgttggcgcg tgagtgcaat ctgcaaacac gtctgggggc gattttgaat gaaactggcg
     9781 atgttatttc cgatattgcg ctctatttac cgtttttatt tttaccagaa agtaacgcat
     9841 cactcgtaat actcatgtta tttttcacca tattgactga gttttgcggt ttactcgcgc
     9901 agacgattaa cggtattcgc agttatgccg gtccattcgg caaaagcgat cgtgcgttaa
     9961 tatttgggtt gtgggggctg gccgttgcca tttatccaca gtggatgcaa tggaataatc
    10021 ttttatgggg cattgcttca attctgctta tctggactgc gattaatcgt tgtcggagtg
    10081 tgctcctgat gagtgctgaa agataatgct ggaaaaatct ctggcaacac ttttcgcgtt
    10141 gttaatttta gcaacgctga taaaccgttt tcgggtatgg cggttaccgg agagaaaaga
    10201 ggatgaagtt acattacgta ttcgtacctg gtggggtatc gtcatttgtt tttcactggt
    10261 gatttcaggt ccacgctgga tgacgttgac gttttttgcg ctgataagtt ttctggcatt
    10321 gaaagaatat tgtacgctta tatccgtaca ttttccgcgt tggttatatt gggttattcc
    10381 tcttaactat ttgcttatcg gttttaattg ctttgagctg tttctgttat ttataccttt
    10441 ggctgggttt ctgatattaa ccacctggcg agttttagta ggtgatacct ccggttttct
    10501 gcataccgtg agcgccattt tttggggctg gataatgacc gttttcgcct tgagtcatgc
    10561 cgcctggtta ttaatgttgc caaccacaaa tacccagggc ggggcgttac tggtgttatt
    10621 tcttttggcg ttaacagagt caaacgatat tgcacagtat ttatggggga aatcctgcgg
    10681 cagaagaaaa gtggtcccca aagtcagccc gggaaaaacg ctggaaggtc tggtgggggg
    10741 cgtaatcacc accatgattg cgtcactgat tattggaccg ttgctgacac cgctaaatac
    10801 attacaggca ttattagcgg gtttgttaat tggtattagt ggtttttgcg gcgatgtagt
    10861 gatgtcagcc gtcaaacgag atgtgggtgt taaagatagc ggaaaactat taccaggaca
    10921 tggcggactc cttgacagga ttgactcatt aattttcacc gctccggtat ttttttattt
    10981 tattcgctac tgctgttact gaaggaaaat agagaaaatg gaaaattcac gcatccctgg
    11041 ggaacatttt tttaccacca gtgataatac agcgttgttt tatcggcact ggcccgcttt
    11101 acagcccggg gcgaaaaagg tcatcgtctt atttcatcgc ggtcatgaac attctggtcg
    11161 tctacaacat atcgttgatg aactggcgat gccagatact gctttttatg catgggatgc
    11221 ccgagggcat ggacaaactt cggggccccg tggttatagc ccatctcttg cgcgttcagt
    11281 gcgggatgtc gatgagtttg tccgttttgc tgccagcgac agccaggtcg ggctggaaga
    11341 ggtggtagtg atcgcgcaga gcgtcggcgc agtactggtt gccacatggg ttcatgatta
    11401 tgcgcctgca attcgcgggc tgctgctggc ttctccggcc tttaaggtta aattgtatgt
    11461 gccgctggca cgtcctgcgc tggcgttatg gcatcgtctg cgtggtctgt tttttattaa
    11521 ttcctatgtg aaaggacgct atttgaccca cgatcggcaa cgggtggcga gtttcaataa
    11581 tgatccgctg atcacacggg cgattgccgt caatatcttg ctcgatcttt ataaaacgtc
    11641 tgaacgtatt gttagcgatg cggcggcgat tacgctcccc acgcaacttc tgatatcagg
    11701 cgatgactat gtggtgcatc gccaaccgca gattgatttt tatcagagat tacgtagccc
    11761 tctgaatgag ctgcatctgc tgcctggctt ttatcacgac acgttgggtg aagagaacag
    11821 ggcgcaggca tttgaaaaaa tgcaaagctt tattagtcgt ttatatgcta acaagtcaca
    11881 aaaatttgat tatcagcatg aagaccgcac tggaccatca gcggatcgct ggcggctcct
    11941 ttcaggtgga cccgtgccat tatcgccggt tgatttggcg tatcgcttta tgcgtaaagc
    12001 gatgaaattg ttcgggacgc actctgcggg cctgcatctc ggaatgagca ccggctttga
    12061 ttcaggcagt tcgctggatt atgtctatca aaatcaaccg caaggtagta acgcattcgg
    12121 gcgcttaatc gacaaaatct acctgaacag tgttggctgg cgcggtattc gccagcgcaa
    12181 aacccattta caaatactga ttaaacaagc cgttgccgat ctccacgcca aaggtttagc
    12241 cgtccgcgtg gttgacattg ccgcagggca tgggcgctat gtactggatg cgctggcaaa
    12301 cgagcctgcc gtaagcgata ttttgttacg tgattacagc gagttaaatg ttgcacaggg
    12361 gcaagagatg attgcgcaac ggggaatgtc tgggcgggtg cgttttgaac agggcgatgc
    12421 gtttaaccca gaggaactca gcgcgttaac tccgcggcct acgctggcga ttgtctctgg
    12481 cctgtatgag ctttttcccg aaaatgagca ggtaaaaaac tcactcgcag gtcttgccaa
    12541 tgccatcgaa ccgggcggca ttctcctcta caccgggcag ccgtggcacc cacaactgga
    12601 gctgattgcc ggggtgttaa ccagtcataa agatggtaaa ccgtgggtaa tgcgcgtgcg
    12661 ttcgcaaggg gagatggatt cgctcgtgcg taatgccgga tttgataaat gcactcaacg
    12721 gattgatgag tggggcattt ttacggtttc gatggcggtg cgtcgtgata actgaacgcc
    12781 ggaacgtatt gctacaaggc gctggctggt tattgttgct gaccccgttt ttcttcttca
    12841 cctatggatc tctaaatcag ttcaccgcgg ttcaggactt taacagccat gatatcccca
    12901 gtcaggtatt cggctgggaa acggcgatcc cttttcttcc ctggactatt gttccttact
    12961 ggagtctgga tcttttatat ggattttcgc tgttcgtttg tagctcgaca ttcgaacagc
    13021 gccgacttgt ccaccggctt attctggcaa cggtaatggc ctgctgcggt ttttttctct
    13081 acccgctgaa gtttagtttt atccgtcctg aagtgagtgg ggtgacagga tggctatttt
    13141 cgcaacttga actgtttgat ctgccttata accagtctcc ttcgctgcat attattctct
    13201 gctggctact ttggcgtcac tttcgtcagc atctggctgt gagttggcgt aaagtctgcg
    13261 gcggatggtt tttactcatc gccatttcga cgctgacaac ctggcagcat cattttattg
    13321 atgtcatcac agggctggcg gtaggtatgt tgattgactg gatggtaccc gtcgaccgtc
    13381 gttggaatta tcagaaacct gatcaacgtc gaatcaaaat agcacagcca tatgtcgtag
    13441 gcgcgtgcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
    13501 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtac ggtgggcttg
    13561 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
    13621 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
    13681 cggtgagcaa aatgactgct ggtgtttatt taggggcgtt tccacgacat attccggcac
    13741 agaatgcggt tctggatgtc acctttgaat tccctcgcgg acgagcgaca aaagatcgac
    13801 tctatttctg tgtaccgatg ctggatctgg tggttccgga agagggggag ctccgacagg
    13861 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
    13921 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
    13981 aaacagttga tgaagcgatt agctttattc gagccagacg ctcgcatatt gtgcttaagg
    14041 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
    14101 gaactacagt ccacatcaag atcgtgtccg gttatgcagc aacaatactg tcgatggctg
    14161 ctttcgcgtc agactgcgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga
    14221 agacaaattt cacatcggta atgccgataa agcccaggaa cgtggacaga tacggcgtca
    14281 ccaggtccgt tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt
    14341 tacccgttac cagaccttcc ggaccattct cggtatagcg gaaagtaacg cctgcacgtg
    14401 caaccaggtc aaaatagttt ttcagctgag tcgagatgtt gaagttatac atcggtgccg
    14461 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agtgccagag
    14521 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc
    14581 catccagtac cggaattgga tttgcagcca ggtcgcgaac ggtgatttca tcagcggagt
    14641 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
    14701 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat ggatatttga
    14761 atgggcgatg ccccgttgct tgttgacact ttattcacaa tcctgccaca gagatagcgc
    14821 aataaatcga agcctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
    14881 ctctatatct atcatttcaa agaaaattaa tcaggcagac tactgcccac taacgttatg
    14941 acagaacaac aaaaattgat ctttacggcc ttgcagcagc ggctggattc gctgatgctg
    15001 cgtgacagac tgcgtttttc tcgccgtctg cacggcgtga agaaggttaa aaatcctgat
    15061 gcacaacagg ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc
    15121 ctgctgcgtg aagcggcacg atcggaaatt acttatcctg acaatttacc ggttagtcag
    15181 aaaaagcagg acattctcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa
    15241 acgggttctg gtaaaacgac tcagttaccg aaaatctgta tggagctggg gcgcgggatt
    15301 aaagggctga tcggccatac ccagccgcgt cgcctggcgg caagaacggt ggcgaaccgt
    15361 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
    15421 gatcacgtaa gtgataacac gatggtcaag ctgatgaccg acggtatcct gctggcggag
    15481 atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
    15541 cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct
    15601 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
    15661 aataatgcgc cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc
    15721 ccgattgttg aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc
    15781 gtagacgaac taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg
    15841 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
    15901 ccgctttatg cgcggctttc gaatagcgaa cagaaccgcg tgttccagtc gcacagcggg
    15961 cgacgcattg tgctggcgac caacgtcgcg gaaacctcgc tgactgtacc ggggattaaa
    16021 tacgttatcg accccggtac ggcgcgtatc agccgctaca gctatcgcac caaagtgcag
    16081 cgtttgccga ttgagcccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt
    16141 cgtgtgtccg aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
    16201 gagtttaccg acccggaaat tctgcgtacc aacctggcct cggttatttt gcagatgacc
    16261 gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat
    16321 atccaggatg gcgtgcgtct gctcgaagag ctgggcgcga tcaccactga tgaacaggcc
    16381 agcgcctata aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt
    16441 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
    16501 acgtccgcgc tctccattca ggatccgcgc gaacgtccga tagacaaaca gcaggcatcg
    16561 gacgaaaaac atcgtcgctt ccacgacaaa gagtctgact tcctcgcgtt tgtgaatctg
    16621 tggaattatc ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt
    16681 cgtaccgatt atctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
    16741 cgccaggtgg tgaaagaact cggcattccg gtgaacagcg aaccggcgga gtatcgtgaa
    16801 attcacattg cgttgctgac ggggttgctt tcccatatcg gcatgaaaga tgccgataaa
    16861 caagaatata ccggcgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
    16921 aaaaaaccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtgggga
    16981 cgtattgcgg cacgcatcga tccagaatgg gtggaacccg tcgctcagca tttgattaaa
    17041 cgtacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
    17101 gtcaccgttt atggtttgcc gattgttgcc gcgcgcaagg tcaactacag ccagatcgat
    17161 ccggcgttat gtcgtgaact ctttattcgc cacgcgctgg tggaaggtga ctggcagacg
    17221 cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gcttgaacac
    17281 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
    17341 cgcatcagcc acgatgtaat ctccgctcgt cacttcgaca gctggtggaa aaaagtcagc
    17401 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
    17461 aagatcagca agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg
    17521 agctatcagt ttgagcccgg cgcggatgct gacggtgtga ccgtacatat tccgctgccg
    17581 ttacttaacc aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa
    17641 ctggtgattg ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtacccgcg
    17701 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgcttgac
    17761 agccttgagc gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac
    17821 tgggatcagg tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag
    17881 aagctaaaag aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag
    17941 gaaacgctgt ctgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt
    18001 tttgggcaac tgccggaaag ctacgagcag aagcgcggca actacaaagt gaaggcgtgg
    18061 ccggcgctgg tggatgagcg cgacagtgtg gcgatcaaac tgtttgataa cccgctggag
    18121 caaaaacagg caatgtggaa cggtcttcgc cgtctactgc tgctgaatat tccatcgccg
    18181 atcaagtatt tacatgagaa gttaccaaac aaagccaagc ttggactgta ctttaacccg
    18241 tatggcaaag tgctggagct gatcgacgac tgtatctcct gcggtgtgga taaattgatc
    18301 gacgccaatg gtggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg
    18361 cgtgccgaac tgaacgacac ggtggtggat attgcgaagc aggtcgagca aatccttacg
    18421 gcagtgttca atatcaacaa gcgtctgaaa gggcgggtgg atatgaccat ggcgctgggg
    18481 ctttctgata ttaaagcgca gatgggcggg ttggtgtatc gcggttttgt gactggtaac
    18541 ggcttcaaac ggctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa
    18601 aaactggcga ttgatccaca tcgcgaccgt gcacagatgc tgaaagtcga aaacgtccag
    18661 caggcgtggc agcaatggat caacaaactt ccgcccgcac gtcgtgagga tgaagacgtg
    18721 aaagagatcc gctggatgat agaagagctg cgcgtcagct attttgccca acaactcggt
    18781 acaccttatc cgatttcaga taagcgtatt ttgcaggcga tggagcaaat tagcggttaa
    18841 cgcggctatt tgcctgataa atataaaacc cggtaagcat ttagcgccgg gttttttatt
    18901 aattctaaaa cggtaagggc aaaaattcag gaattcagaa aaatacaatt ctctgctgca
    18961 agatgaataa tgtttatcta cagcatttcc ttaaaagata tgtcaggctt gcggagtggc
    19021 ggttaaggaa atacgacatc ctcctttcag agtgctccgc ttctcactat tatctcacgc
    19081 agtattctta agggaacgat aaggaggaac catgaacatt accccgtttc cgacgctttc
    19141 gacggcaact atagatgcca taaatgttat cggacagtgg ctggcgcagg atgatttctc
    19201 cggtgaggtg ccgtatcagg ctgattgcgt catacttgca ggaaatgcgg ttatgccgac
    19261 tatcgatgcg gcatgtaaga ttgctcgcga tcagcaaatt cctttactga ttagtggtgg
    19321 tatcggtcac tcgaccactt ttttgtatag cgccatcgca cagcatccgc actacaacac
    19381 tatccgcacc actggcagag cagaagcgac catcctggcg gatatcgctc atcagttctg
    19441 gcatattccg catgaaaaaa tctggattga agaccagtca acaaactgcg gtgaaaacgc
    19501 acgctttagc atcgcgctat tgaatcaggc cgtagaacga gttcatacgg ctatcgttgt
    19561 acaggacccc accatgcagc ggcgcacgat ggcaacgttc cgccgtatga ccggggacaa
    19621 tcccgatgca ccacgctggt taagttatcc cggattcgtt cctcagttag gaaataacgc
    19681 agacagtgta atctttatta atcagttaca aggattatgg ccggttgagc gttatctctc
    19741 actactcact ggcgagctgc cgcgtttacg cgatgatagc gatggctacg gtccccgcgg
    19801 gcgagatttt atcgttcacg ttgattttcc ggcagaagtc attcaggcat ggcaaacgct
    19861 taaacacgat gcggtgctca tcgaggcgat ggaaagtcgc tcgttacgtt aaaaattgcc
    19921 cgtttgtgaa ccacttgttt gcaaacgggc atgactcctg acttttattt ctgcctttta
    19981 ttccttttac acttgttttt atgaagccct tcacagaatt gtcctttcac gatgccgtct
    20041 ctatgatgat tgatgttaat taacaatgta tttaccaaaa acaaacatat aaatcacagg
    20101 agtcgcccat gtcagtaccc gttcaacatc ctatgtattt tgatggacag tttgttacct
    20161 ggcgtggaga cgcatggatt gatgtggtaa accctgctac agaggctgtc atttcccgca
    20221 ttcccgatgg tcaggccgag gatgcccgta aggcaatcga tgcagcagaa cgtgcacaac
    20281 cagaatggga ggcgttgcct gcaattgaac gcgccagttg gttgcgcaaa atctccgccg
    20341 ggatccgcga acgcgccagt gaaatcagtg cgctgattgt tgaagaaggg ggcaagatcc
    20401 agcagctggc tgaagtcgaa gtggctttta ctgccgacta tatcgattac atggcggagt
    20461 gggcacggcg ttacgagggc gagattattc aaagcgatcg tccaggagaa aatattcttc
    20521 tgtttaaacg tgcgcttggt gtgactaccg gcattctgcc gtggaacttc ccgttcttcc
    20581 tcattgcccg caaaatggct cccgctcttt tgactggtaa taccattgtc attaaaccca
    20641 gtgaatttac gccaaacaat gcgattgcat tcgccaaaat cgtcgatgaa ataggccttc
    20701 cgcgcggcgt gtttaacctt gttctggggc gtggtgaaac cgttgggcaa gaactggcgg
    20761 gtaacccaaa ggtcgcaatg gtcagtatga caggcagcgt ctctgcaggt gagaagatca
    20821 tggcgactgc ggcgaaaaac atcaccaaag tgtgcctgga actggggggt aaagcaccag
    20881 ctatcgtaat ggacgatgcc gatcttgaac tggcagtcaa agccatcgtt gattcacgtg
    20941 tcattaatag tgggcaagtg tgtaactgtg cagaacgtgt ttatgtacag aaaggcattt
    21001 atgatcagtt cgtcaatcgg ctgggtgaag cgatgcaggc ggttcaattt ggtaaccccg
    21061 ctgaacgcaa cgacattgcg atggggccgt tgattaacgc cgcggcgctg gaaagggtcg
    21121 agcaaaaagt ggcgcgcgca gtagaagaag gggcgagagt ggcgttgggt ggcaaagcgg
    21181 tagaggggaa aggatattat tatccgccga cattgctgct ggatgttcgc caggaaatgt
    21241 cgattatgta tgaggaaacc tttggcccgg ttctgccggt agtcgcattt gacacgctgg
    21301 aagatgctat ctcaatggct aatgacagtg attacggcct gacctcatca atctataccc
    21361 aaaatctgaa cgtcgcgatg aaagccatta aagggctgaa gtttggtgaa acttacatca
    21421 accgtgaaaa cttcgaagct atgcaaggct tccacgccgg atggcgtaaa tccggtattg
    21481 gcggcgcaga tggtaaacat ggcctgcatg aatatctgca gacccaggtg gtttatttac
    21541 agtcttaatg agtgaaagag gcggaggttt tttcctccgc ctgtgcgcgt cagagtttag
    21601 cgaatttttc gagggtgcga ataagctgcg tgacgaagcc atattcgtta tcgtaccagg
    21661 cgaccgtttt caccagttgt aaatcgccca cggcggtaat ttccgtttgc gtggcatcaa
    21721 ataccgaacc gaaatggctg ccaatgatat cggaagagac tatttcttca tcggtataac
    21781 caaatgactc gttattggtg gttgcttgtt taagtgcgtt attcacctct tcggcagtca
    21841 ctttttttcc gagaatcgat accagttcag tgaccgaacc tgttttcacc ggcacgcgtt
    21901 gcgcatgacc tttcagtttg ccgctcagtt ccgggatcac cagaccaatg gcttttgccg
    21961 cgcccgtagt gtggggaatg atattttctg ccgctgcgcg tgaagcacgt aaatctttac
    22021 cacgcgggcc atccaccagt gactgggtgc cagtataggc atgaatggtc gtcatcgtgc
    22081 cgacttctat cccgaaactg tcatgcaagg ctttggccat cggcgcaagg cagttagtgg
    22141 tgcatgacgc cacggaaaca atggtatcgt tgccatccag agtgtcgtca ttgacgttat
    22201 aaacgatagt tttcatttca ccggcagggg cggaaatcaa caccttcttc gcgccagcat
    22261 caagatgcgc ctgcgatttc tcggcggagg tataaaagcc agtacattcg acaatgattt
    22321 ctgcaccttt cgctttccac ggaatatttt tagcctcttt ttcggcgtaa accgcgatac
    22381 ttttaccatc aacgataagt gaatcttccg taaaatcaac gctccagggg aacggaccat
    22441 agtttgaatc atgtttcagc agataggcga gaatttttgg ggaagtaaga tcattaatag
    22501 cgacaacgtc tatgttgctt ttgacttcaa gtaaacgacg caacaccagt cgaccgatac
    22561 gaccaaaacc gttaatacca actttactca tggttttctc ctgtcaggaa cgttcggatg
    22621 aaaattgatc ctttccaggc ttagaccagg attgtgggat gggcaatctc cattcttaca
    22681 gtgaaacgta acgtactgaa aacgggtgaa caatatttaa tgaaattttg agaaaagccc
    22741 gttatgttaa cggaaaatta tgttaaagca ggaaatgtta tggaaaataa atactcaagg
    22801 ttacaaatcg gcattcactg gctggtcttt ttgctggtta tcgcagcgta ttgcgcaatg
    22861 gagtttcgtg gtttcttccc acgtagcgat cggccgctca tcaacatgat tcatgtttcc
    22921 tgtggcatct caattctcgt gctgatggtg gttcgtcttt tgttaagact gaaataccca
    22981 accccgccga ttatacctaa gccgaaaccg atgatgacgg gactggcgca tttgggacat
    23041 ttggtgattt atctcctgtt tattgcgctg ccagtgattg gtttggtgat gatgtataac
    23101 cggggcaacc cgtggtttgc gtttggtttg acgatgcctt acgcttcaga ggccaatttc
    23161 gaacgggtag atagcttaaa gtcgtggcat gaaacgctgg cgaatctggg atattttgtc
    23221 atcgggttgc acgctgcggc tgcactggca caccactatt tctggaagga caacacactt
    23281 ctacgcatga tgccgcgtaa acgttcctga aggatattta aagaaaacgc ctgtactaaa
    23341 accgacccgt ggtacaggcg aagaatacgg gtctacatcg gaagcgccta ttatatttat
    23401 ttgtatgaca aataaaacca taatccttgc ccatacgtcc atctggctta tttttaatca
    23461 attcacccga tctttgatct catcaacggt atcaaaataa accagcgtat aacgttcaaa
    23521 tcgaacataa gattcgataa accatggagg ttatatgaaa aaactggcac ttattttgtt
    23581 tatgggagcg cttgtttcct tttatgccga tgccgggcgc aaaccctgtt ctggttcgaa
    23641 aggggggatc tcacactgta cggcaggcgg caaatttgtc tgtaatgatg gttctattag
    23701 tgcatcgaaa aaaacatgca ctaactgaag tgtaaaaggg gtgccatgag aaaatgaatc
    23761 tgctagtcag atgcgcgggg aaaatccccg cgcttgccct tacctggacg tgcaggccat
    23821 gagcgcagca acctccttat caccgtcccg gaaccgcagt tcgtagagcg tttgtcgggt
    23881 caggagtgtg aatgtcagaa tcgtaatgca gataatgagc agacacacca ccagagggtt
    23941 gcgcttcata gcctttctcc ttgccggatg gcgggtaaga ggctaacatc tgaattgctt
    24001 ggttcattcg ttggcctcgg ttgatagaaa tatcggtcgg ggccttcgtc tttctgattc
    24061 ccggttagcc tgaaaacaga aagtctcagg cacccgtgag catcctatgt ggtttcctta
    24121 gggacgaaaa taatcacctg acgaaattgc gtgctgtttt ccagaatttt tcgtcattcg
    24181 ggttagccag tttaaccatt cgttactctc ttcattccaa tggcattaat cttctctgca
    24241 ataattgttg taaaaatgtg acgcaaagag gcttttagtc gtaagtaatt accgtcaaat
    24301 gccgatgact ttctatcagg agtaaacctg gacgagagac aacggtaatg aatacaactc
    24361 cctcacagcg attaggtttt ttgcatcaca tcaggttggt tccgttattt gcctgcattc
    24421 taggcggtat cttagttcta ttcgcattaa gttcagccct ggctggctat ttcctctggc
    24481 aggccgatcg cgatcagcgt gatgttactg cggagattga aattcggacc gggttagcga
    24541 acagttcaga ttttttgcgt tcagcccgga tcaatatgat tcaggccggg gctgcgagtc
    24601 gtattgcgga aatggaagca atgaagcgaa atattgcgca agccgaatcg gagattaaac
    24661 agtcgcagca aggttatcgt gcttatcaga ataggccggt gaaaacacct gctgatgaag
    24721 ccctcgacac tgaattaaat caacgcttcc aggcttatat cacgggtatg caacctatga
    24781 tgaaatatgc caaaaatggc atgtttgaag cgattatcaa tcatgaaagt gagcagatcc
    24841 gaccactgga taatgcttat accgatattt tgaacaaagc cgttaagata cgtagcacca
    24901 gagccaacca actggcggaa ctggcccatc agcgcaccag cctgggtggg atgtttatga
    24961 ttggcgcgtt tgtgcttgcc ctggtcatga cgctgataac atttatggtg ctacgtcgga
    25021 tcgtcattcg tccactgcaa catgccgcac aacggattga aaaaatcgcc agtggcgatc
    25081 tgacgatgaa tgatgaaccg gcgggtcgta atgaaatcgg tcgcttaagt cgtcatttac
    25141 agcaaatgca gcattcactg gggatgacag tagggactgt tcgacagggc gcggaagaga
    25201 tttatcgtgg caccagcgaa atttccgctg gcaatgcgga cctttcatct cgcaccgaag
    25261 aacaagcggc ggcaattgaa caaactgccg ccagcatgga gcaactcact gcgacggtga
    25321 aacagaatgc ggataacgcg cattatgcca gtaaactggc gcaagaggct tctattaaag
    25381 ccagcgatgg cgggcagacg gtttccggtg tagtaaaaac gatgggcgct atctccacga
    25441 gttcgaagaa aatttctgag atcaccgccg tcatcaacag tattgctttc cagacgaata
    25501 ttctggcact gaatgctgcc gttgaagctg cgcgagcggg tgagcaaggg cgtggatttg
    25561 ccgttgtcgc cagcgaagta cggacactcg caagccgcag cgcccaggcg gcgaaagaga
    25621 ttgaaggctt gatcagtgaa tcagtcaggt taattgacct gggatcggat gaggtggcaa
    25681 cggccgggaa aaccatgagc actattgttg atgccgtcgc gagtgtcaca catatcatgc
    25741 aggaaatcgc cgccgcctcg gatgagcaaa gtagaggcat aactcaggtt agccaggcga
    25801 tttctgaaat ggataaggtg acgcaacaga atgcttctct ggtagaagag gcctcagcag
    25861 cggcggtgtc ccttgaagaa caggctgcac gattaactga ggcggtggac gtattccgtc
    25921 tgaacaaaca ttctgtgtcg gcagaacctc gcggagcggg tgaacaagtt agtttcgcta
    25981 cggtgtgaaa atgttcaagg agggatcgac agatcccttc acctttcaga acggcattga
    26041 ttttcgaata gcgttaatca tcaactggca accagaagag aacgtcgcat ctacgcgggt
    26101 cagtattcca atcggttcgc ctgcaccatg tcccggaaca ggcagggcca ccagcgtggc
    26161 atgacgcagg tcgtctttta ccgcgccaga agggacaaac cacacgtagt cgtattcaac
    26221 cgtaagttga cgagatagcg aagcagacag cgtttcgata caacccgaag gaattttaca
    26281 tccctggctc tgcactaatg catctgaatg ctggcgtggc gcagtgcctt ctggtgatac
    26341 aacgaccggc cattccagca cccggcttag cgttacgttc tcctgaagta gcgggtgatt
    26401 agggcggaca accagcttca gcgattcaag aaacagcagt tcgtaattaa gcccggtcat
    26461 cagttcagga tctgacatcc gaccaatgcc gatatcgatt tccccggttt tcaaacccgc
    26521 cagaatcata gggttactca ttgtcgcaac ttgcaaggtc gtctcttttt gttgctgatg
    26581 aaactgacct ataaccgaag gtaatatccc cagtgccgca gtaggtagtg caccaaccct
    26641 gacgacatca ttattaggac cttctttacg attaagcgac tgtccggcag tgttgatggc
    26701 gtcaagaact ctgactgcat gcgttaaaaa ttgttcgccg ggtaaggtaa gttgcgcccc
    26761 ctgacgacca cgctcaaaca agcgagcgcc cgtcagctgc tccagttcat tcaatgtttt
    26821 agagagcgca ggttgactca aattaagggt ttcagccgcg cgccccaaag ttccttgttg
    26881 tgcgacagct acgaatgtat gaaggtggcg caaacggatg cgctgactaa acagactatt
    26941 tttttccata agcgatgtta aaaacgaagc ggtgtcgctg acaagtgaag ttgtttgatt
    27001 atgataactt gattgcaaaa tattattaac aattaaagca attatgttac agcaaaatgg
    27061 ataatattga tgttttcgcg gcgagatcac agtttgtaaa ttcttcccgc cagagtgaag
    27121 gcggttacct acactccaga ttactgacca ctggaggcag acactatggc gaacagcatc
    27181 acggcggatg agattcggga acagttttcg caggcaatgt cagccatgta ccagcaagaa
    27241 gttccgcaat atggcacgct gctggaactg gtagctgatg tgaatctggc tgtgctggaa
    27301 aacaatcctc aactgcacga aaaaatggta aatgcagacg agctggcgcg gctgaatgtt
    27361 gaacgtcatg gggcgattcg cgttgggact gcacaagagc ttgctactct tcggcggatg
    27421 tttgccatta tggggatgta cccggtgagc tattacgatc tctcgcaggc aggggtgccg
    27481 gtacattcga cagcatttcg gcccattgat gatgcttctc tggcgcgtaa tcccttccgc
    27541 gtttttacct ccttactccg ccttgagctt atcgagaacg aaattttgcg ccagaaagcg
    27601 gcggagattc tgcgtcagcg cgatatcttc accccacgtt gtcgacaact gttagaggaa
    27661 tatgagcagc agggcggttt taacgaaaca caggcacagg agtttgtgca ggaggccctg
    27721 gaaacgtttc gctggcacca gtcagcaacg gtagatgacg aaacctatcg cgccttgcac
    27781 aacgaacatc ggttgattgc tgatgtggtc tgttttcctg gatgccatat caaccatctg
    27841 acgccacgta cgctggatat tgaccgggtg cagtcgatga tgcctgaatg cggaattgaa
    27901 ccaaaaattc tgatcgaagg gccgccgcgc cgcgaggtac cgattttact acgccagacc
    27961 agctttaaag cactggaaga gacggtgttg tttgcggggc agaaacaggg cacgcatact
    28021 gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa cgccgaaagg gcgacaactg
    28081 tatgatgatc ttctgcgtaa cgctggaacc gggcaggata atctcactca ccaaatgcat
    28141 ttacaggaaa ccttccgcac ttttcctgac agtgagtttt taatgcgtca gcaagggctg
    28201 gcatggttcc ggtaccgtct gacgccttcg ggtgaggcgc atcgtcaggc gattcatccc
    28261 ggagacgatc cacaaccctt aattgaacgt ggttgggtag tggcgcaacc cattacctat
    28321 gaagatttct tgcccgttag cgcggcgggg atcttccagt caaatctggg taatgaaacg
    28381 caggcacgca gccacggtaa tgccagtcgc gaagcatttg agcaggcgtt gggttgtccg
    28441 gttttggatg agttccagct ttatcaggaa gcggaagaac gcagtaaacg tcgctgtggt
    28501 ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc ctgattttac aaacgaatca
    28561 ggctcatccc atcgacataa aaaaattgcc gatttatgca tattctctga gttcaacaat
    28621 tggattatta ataaatattg tctagagtga gcggtcataa ataagcactt tcttgccgct
    28681 gaaaacgacc agcgcgggac cattcacaac accagaagga ctcactttca ggtatggatc
    28741 gtagacgatt tattaaaggt tcaatggcta tggccgccgt gtgcggtacc agcggcattg
    28801 cttctctttt ttctcaggcg gcattcgcgg cagattctga tattgccgac gggcaaaccc
    28861 agcgttttga cttctccatt ctacagtcaa tggcgcacga cttagcgcaa acagcgtggc
    28921 gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat gacgccgcag gcttataaca
    28981 gtattcaata cgacaccgaa aaatcgctct ggcataacgt tgagaaccgt caactggacg
    29041 ctcagttctt ccatatggga atgggattcc gtcgccgcgt tcgtatgttt tctgtagatc
    29101 cagcaacaca tctggcgcgt gaaattcact ttcgcccgga gttgttcaaa tacaacgatg
    29161 caggtgttga taccaaacaa ttagaagggc aaagcgatct cggctttgcc ggttttcgcg
    29221 tgtttaaagc ccccgaactg gcgcgtcgtg atgtagtatc attcctcggt gcgagttatt
    29281 tccgcgccgt tgatgacaca tatcaatacg gtttgtcggc ccgcggcctg gcgattgaca
    29341 cttacaccga cagtaaagaa gagttccccg actttaccgc cttctggttt gatacggtaa
    29401 aaccgggggc aactaccttt accgtttatg cgttgctcga tagcgccagc attactggtg
    29461 cctataagtt cactatccat tgtgagaaaa gtcaggtgat tatggatgtg gaaaatcacc
    29521 tgtatgcgcg caaagacatt aaacagctgg gcattgcgcc gatgaccagc atgttcagct
    29581 gcggtactaa tgaacgtcgg atgtgcgaca ccattcatcc gcaaattcat gactctgatc
    29641 gtctgtccat gtggcggggc aacggcgagt ggatttgccg tccgctgaat aatccgcaaa
    29701 aattgcagtt caatgcttac accgacaaca acccgaaagg gtttggttta ttgcaactgg
    29761 atcgtgactt ctcccattat caggacatta tgggctggta taacaaacgc ccaagtctgt
    29821 gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg cctgatggaa atcccaacaa
    29881 cgggcgaaac gctggataac attgtctgct tctggcagcc agaaaaagct gtaaaggcgg
    29941 gtgatgagtt tgcattccag tatcgtctgt actggagtgc gcaaccgcct gttcattgcc
    30001 cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg tttcccggaa ggttgggcac
    30061 caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc cgtcgatttc gttggtggtg
    30121 atctgaaagc tgccgcacca aaaggcattg agccggtgat tacgctttcc agtggggaag
    30181 cgaagcaaat cgaaattctc tatattgaac ccattgatgg ttatcgtatt cagtttgact
    30241 ggtatccgac gtcggactcc actgatccgg tcgatatgcg gatgtatctg cgttgtcagg
    30301 gcgacgctat cagtgaaaca tggctgtatc agtatttccc gccagcgccg gataaacgtc
    30361 agtatgttga cgaccgcgtg atgagttaat cgttttttct tcggcacctt cttcgggagg
    30421 tgccgtctgg ttaaacacga tcccgctcgc atttttccct aagttaaatg agtaatctga
    30481 tggtgtgtat ttcagataca ccttgtcagc cactaacagg gagtgcgtat gtttccagaa
    30541 taccgagatt taatatcccg tctgaaaaac gaaaatcctc gctttatgtc cttgttcgat
    30601 aaacacaata aacttgatca tgaaattgcc agaaaggaag gttccgacgg tcgagggtac
    30661 aatgcggaag tggtccgcat gaaaaaacaa aagctacagt taaaagatga gatgctcaaa
    30721 atcctgcagc aggagagcat caaagaggtg taaactttcc taagccgcca gccaggcggc
    30781 ttttttaaca actgcatgga ttgactggag ataagatgac tgaaacgata aaagtaagcg
    30841 aatcaattga attacatgct gttgcagaaa gtcatgtcac acctctttat cagttaatct
    30901 gtaaaaataa aacctggtta cagcagtcgc taaactggcc gcagttcgtt cagagtgaag
    30961 aggacacgcg aaaaacggtg cagggtaatg tgatgttgca tcaacgcggc tatgccaaaa
    31021 tgttcatgat tttcaaagaa gatgaactta tcggcgttat ctcgtttaat cgtattgaac
    31081 cactgaataa aaccgctgaa attggctact ggctggacga atctcatcag gggcagggga
    31141 tcatttctca ggcgctgcag gcattgattc atcattacgc tcagtctggt gaacttagac
    31201 gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa ccaggtcgct ttgcgcaatg
    31261 gttttatcct tgaaggttgc ctgaaacagg ctgagttcct gaatgatgcc tatgatgatg
    31321 tgaacttata cgcgcgtatt atcgattcac aataaccctg ccagcggcgt tcgcgtaatg
    31381 cgctcctcgc cgttaatgac ttttggacca cggacatgaa ccgtgtcgcc atcaaaggct
    31441 tcaacgacgg cgtggtcggt taactccaca tggttttcaa ttatcaccgc acctgtaata
    31501 cggctcttac cttgtattac aacgtgctcg tcgagcagaa tcggtccgcc acgtaccacg
    31561 gcatttccgc caatcaagac atgatgtttt aacacacaat taccttccac aatggcatat
    31621 tccgccacct gtgaactgta atgaatcgtc ggaatggcat cttcttctat gccagctttc
    31681 acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc agacgttgtt ctcttcatta
    31741 ccttcaacac tggcaaaatc aaaaacttcg gcgcgatgtt caataaaagc ataccggatg
    31801 acggcatcgc cataaatttg tgcctggtgg acaatacgcg aggcgctaac ccttgcgcga
    31861 tcataaattt gtagcaggag ttgatggtca ggcgttaagc cttgtgctgc gacgatcata
    31921 gaatgttgat taattaaggc gtgaccaaat attctacatt gcccgcaaac cagtgattca
    31981 tgaatggtga cactgtcact tatatatgca ccctggctga tttcactatt atcgatccag
    32041 acattatccg ttgcgtaaac ctctccccac agcacgctgg tgccggtgat gcgcgtatta
    32101 ccggaaatca ccgctccgcc gaacgcaata gcattctggt cgtaaatcca gcaatttcct
    32161 tcctgtgcta acaccgtttc gcgatcgatc cagccgccag cggttcctgc tatcacatcg
    32221 ttaaaatcgc tcatggcgat aatctgccgt aataacacac tttttttagt gccatcatcc
    32281 tgataactaa aggctcgctg ttcttcactt agacgatatt tgcgcatagc gttttcccac
    32341 aggtgactta ctataaccgt agcaaattct gcggctctgg ctatgctcga gaaaattcca
    32401 taaaatgcat ttcaaatata ctttataaat taaacaaaat gagtaagaag atgcagagcg
    32461 ataaagtgct caatttgccg gcaggctact ttggtattgt gttggggacg atagggatgg
    32521 gatttgcctg gcgctatgcc agtcaggttt ggcaggtcag ccactggtta ggggatgggc
    32581 tggtgattct ggcgatgatc atctggggat tattgactag cgcatttatt acccgactca
    32641 tacgctttcc gcatagcgtg ctggcggaag ttcgccatcc agtgctgagc agttttgtga
    32701 gtttgtttcc ggcaacgacg atgctggtgg cgattggttt tgttccgtgg tttcgcccac
    32761 tggcggtgtg cctgttcagt tttggtgtcg tggttcagtt ggcttatgcc gcctggcaaa
    32821 ctgcgggatt atggcgcgga tctcaccctg aagaagctac cacgcctgga ctgtatctgc
    32881 cgacagttgc caacaacttt atcagcgcaa tggcctgtgg tgcgttgggc tacaccgacg
    32941 ccggtctggt gtttttaggc gcaggcgttt tctcatggct aagccttgaa ccggtgatct
    33001 tgcagcgtct gcgcagttcg ggagaattac ccacggcact gcggacatct ctcggcattc
    33061 agctcgctcc tgcgctggtg gcctgtagtg cctggctgag cgtcaacggc ggcgagggtg
    33121 acacgctggc gaaaatgctt ttcggttatg gactgctgca actgctgttt atgctacgtc
    33181 tgatgccatg gtatctctcc cagccattta atgcttcatt ctggagtttc tcgttcggcg
    33241 tatctgcact ggcaaccacc ggtttgcatc tggggagtgg cagcgataat ggatttttcc
    33301 atacgctggc ggtgccgctg tttatcttta ccaattttat tattgcaata ctgctcatcc
    33361 gtacttttgc acttctgatg cagggaaaat tgttagtcag aaccgagcgc gccgttttaa
    33421 tgaaagcaga ggacaaagaa tgatcattcg tgacgaaaac tattttactg ataaatatga
    33481 attaacccgc acacactctg aagtactgga agcggtgaaa gtggttaaac cgggtaaaac
    33541 gctggatctg ggctgtggca atggtcgtaa cagtctttac ctggcagcca atggttatga
    33601 tgttgacgca tgggataaaa atgccatgag tatcgccaac gtcgagcgca ttaaatctat
    33661 tgaaaatctg gataatttac acactcgggt cgttgatctg aataacctca catttgctgg
    33721 acagtacgat tttattcttt cgactgtggt gctgatgttc cttgaggcta aaaccatccc
    33781 agggctgatt gccaatatgc aacgttgcac gaaacctggc ggttacaacc tgattgtggc
    33841 ggcgatggat accgctgatt atccatgtac cgtcggcttc ccgtttgcct tcaaagagag
    33901 agaattacgt cgatattacg aaggctggga gatggtgaaa tacaatgaag acgtcggcga
    33961 gctgcaccgc accgacgcca acggtaatcg tattaaactg cgtttcgcca cgatgctggc
    34021 acgtaaaaaa tgacccggta agaacaaagc gcgtgaaaat tccccacgct gagatggttt
    34081 actgttcttc ttttcggtaa gcatattttt tatcgaaggg atgtgaaatt aatcacagta
    34141 gtcgaagttt tttagcagct taacttactg aaatttaact actgatgatt gacttagccc
    34201 ctttttcggc attgactatg tcgtctgaaa aggggctgaa aaatttattt tcaccaacac
    34261 tttttttgcc acaacacgaa gcggcgcttt ttgctataac ttaaaaagta atataatcat
    34321 ctcaggaaac tattcatgcg taccacatca tttgcgaaag ttgcagcttt atgcggcttg
    34381 ttggctctgt ct
//
LOCUS       DAZFFG010000037        33840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFFG010000037 DAZFFG010000000
VERSION     DAZFFG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.037"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1016)
                     /locus_tag="ACHJ7J_002175"
     CDS             complement(<1..1016)
                     /locus_tag="ACHJ7J_002175"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO5000409.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREG"
     gene            complement(1227..2624)
                     /gene="ccp"
                     /locus_tag="ACHJ7J_002176"
     CDS             complement(1227..2624)
                     /gene="ccp"
                     /locus_tag="ACHJ7J_002176"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO5000410.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3029..4678
                     /locus_tag="ACHJ7J_002177"
     CDS             3029..4678
                     /locus_tag="ACHJ7J_002177"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5000411.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4728..5330)
                     /locus_tag="ACHJ7J_002178"
     CDS             complement(4728..5330)
                     /locus_tag="ACHJ7J_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5000412.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLEN
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5850..6749
                     /locus_tag="ACHJ7J_002179"
     CDS             5850..6749
                     /locus_tag="ACHJ7J_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5000413.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGAMKEYLD"
     gene            6798..7811
                     /gene="yhjD"
                     /locus_tag="ACHJ7J_002180"
     CDS             6798..7811
                     /gene="yhjD"
                     /locus_tag="ACHJ7J_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO5000414.1"
                     /translation="MTQENEIKRPTQDLEHKPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPIIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            8204..9526
                     /locus_tag="ACHJ7J_002181"
     CDS             8204..9526
                     /locus_tag="ACHJ7J_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5000415.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9708..11768)
                     /locus_tag="ACHJ7J_002182"
     CDS             complement(9708..11768)
                     /locus_tag="ACHJ7J_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5000416.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11838..12605)
                     /gene="pdeH"
                     /locus_tag="ACHJ7J_002183"
     CDS             complement(11838..12605)
                     /gene="pdeH"
                     /locus_tag="ACHJ7J_002183"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO5000417.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSSEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12837..13766
                     /gene="kdgK"
                     /locus_tag="ACHJ7J_002184"
     CDS             12837..13766
                     /gene="kdgK"
                     /locus_tag="ACHJ7J_002184"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO5000418.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICAELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13862..15358)
                     /locus_tag="ACHJ7J_002185"
     CDS             complement(13862..15358)
                     /locus_tag="ACHJ7J_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5000419.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15579..16865)
                     /gene="dctA"
                     /locus_tag="ACHJ7J_002186"
     CDS             complement(15579..16865)
                     /gene="dctA"
                     /locus_tag="ACHJ7J_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO5000420.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(17048..19036)
                     /gene="hmsP"
                     /locus_tag="ACHJ7J_002187"
     CDS             complement(17048..19036)
                     /gene="hmsP"
                     /locus_tag="ACHJ7J_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO5000421.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(19118..22591)
                     /gene="bcsC"
                     /locus_tag="ACHJ7J_002188"
     CDS             complement(19118..22591)
                     /gene="bcsC"
                     /locus_tag="ACHJ7J_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO5000422.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQLTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKSAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKVIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(22573..23679)
                     /gene="bcsZ"
                     /locus_tag="ACHJ7J_002189"
     CDS             complement(22573..23679)
                     /gene="bcsZ"
                     /locus_tag="ACHJ7J_002189"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO5000423.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(23686..26025)
                     /gene="bcsB"
                     /locus_tag="ACHJ7J_002190"
     CDS             complement(23686..26025)
                     /gene="bcsB"
                     /locus_tag="ACHJ7J_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO5000424.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAIAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNNAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(26036..28654)
                     /gene="bcsA"
                     /locus_tag="ACHJ7J_002191"
     CDS             complement(26036..28654)
                     /gene="bcsA"
                     /locus_tag="ACHJ7J_002191"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO5000425.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWLILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(28651..29403)
                     /gene="bcsQ"
                     /locus_tag="ACHJ7J_002192"
     CDS             complement(28651..29403)
                     /gene="bcsQ"
                     /locus_tag="ACHJ7J_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO5000426.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKSSGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSKS"
     gene            complement(29415..29603)
                     /gene="bcsR"
                     /locus_tag="ACHJ7J_002193"
     CDS             complement(29415..29603)
                     /gene="bcsR"
                     /locus_tag="ACHJ7J_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO5000427.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            29876..31447
                     /gene="bcsE"
                     /locus_tag="ACHJ7J_002194"
     CDS             29876..31447
                     /gene="bcsE"
                     /locus_tag="ACHJ7J_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO5000428.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTPSLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            31444..31635
                     /gene="bcsF"
                     /locus_tag="ACHJ7J_002195"
     CDS             31444..31635
                     /gene="bcsF"
                     /locus_tag="ACHJ7J_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO5000429.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            31632..33311
                     /gene="bcsG"
                     /locus_tag="ACHJ7J_002196"
     CDS             31632..33311
                     /gene="bcsG"
                     /locus_tag="ACHJ7J_002196"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO5000430.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTENNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(33398..33505)
                     /locus_tag="ACHJ7J_002197"
     CDS             complement(33398..33505)
                     /locus_tag="ACHJ7J_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5000431.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         7994 a         9254 c         8495 g         8097 t
ORIGIN      
        1 tagccttcac gaccgaggcg caggaattca tagtactgtg caattacctg acccgccggg
       61 cgggagaagt tgatggcgaa agtaccaatc tgaccaccga ggtagtcaac gttgaacacc
      121 agttcctgcg gcagcgcttc ttcatcacgc cagataaccc agccgcagcc cagcggagcc
      181 agaccgaatt tatggcctga agcactgatc gatttcacac gcggcaggcg gaagtcccag
      241 acgatatccg gggcgacgaa cggtgccagg aagccaccgc tggcggcgtc gatgtgcatg
      301 tcgatgtcga taccggtgtc ggcctggaat ttatccagcg catcgtgcag cggttgcggg
      361 aactcatagt taccggtgta ggtcacgccg aaagtcggca ccacgccgat ggtgttttcg
      421 tcgcaggctt caatcatgcg tttcgggtcc ataaacaact gaccggggcg catagggatc
      481 tcacgcagct ccacatccca gtagcgggcg aatttatgcc agcagatttg taccggaccg
      541 cacaccaggt ttggtttatc cgttggtttg cctgcagctt ccatacgctt gcgccaacgc
      601 catttcatcg ccatcccgcc gagcatacag gcctcggaag aaccaatggt gttggtgcca
      661 acggcctgac catttttcgg cgcaggcgca tgccacagat cggcaaccat gtttacgcaa
      721 cgcaggtcga tggctgcgga ttgcggatat tcttctttgt cgatccagtt tttattgatc
      781 gacaaatcca tcaatttatg gacgttttcg tcgtcccagg tctggcagaa agtggccagg
      841 ttctgacgag cgttgccatc aagatataat tcatcattga taatctggaa tgcgacatca
      901 tcgcgcattt cgtgcagcgg aaatcgtttt gactccgcga tagtagaaat ggcctttgcg
      961 ccaaaacgtg aatcgagtag ttctgagcgg aaatccgtta acagcttctg gtccatttcg
     1021 aactccttaa atttatttga aggcaataaa aaagtaggat ttatccgcaa tggaagcaag
     1081 gcattacagg cttaatttaa ataacaaaat cctaagaaga aaaatgatct tttataatat
     1141 aaacatttaa catgataata ttaaaaacag actttattat cgtaataaaa atcgcccaaa
     1201 cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg cgtgtacacc
     1261 ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg cagctctttg
     1321 cctacctgat agcgcaacat cagtttcacc gccccgtcca gcgtcggcac gtcaccgcga
     1381 tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg acgcaattta
     1441 tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat ttccccaaag
     1501 ttaaagtctt ttttcagccc caacggttcg aaggaacgtc cgccgagaat aattccacca
     1561 tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg ttgcgccgtc
     1621 agcgcattct catctccacg caaccattta tcaaatgggg aatccggcgt aattaatgtt
     1681 ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg cggatagact
     1741 tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat ttcgtcccag
     1801 gattttgacg ccatttcaat cgggttcaac ggcggtccac cagcctgatc ctgcaatgtt
     1861 gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac cgtcggcgcg
     1921 ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc gacgcccccc
     1981 gcgttcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa acggggatcg
     2041 tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt ttgcgggatg
     2101 ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta atattccgcg
     2161 cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac cttacccgcc
     2221 cagtgtagcg cggtataacg cgttggcggc atagtttcat actgcatcac ccattcaatc
     2281 ttattcagat cgctttgcga aacgggttta tcagccagca gagccgcacg cacggcctcg
     2341 aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt cgcgccagga
     2401 atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca tcctttttcg
     2461 cggagaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac atcggcctgt
     2521 ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata gcaaatcgcg
     2581 acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct tccctcacgg
     2641 tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc tgcgagacaa
     2701 agccatcata aaaaacaggg agatacactt tttgatagga atcaatcaat tacatgaata
     2761 taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca tatcatgatt
     2821 gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acacgaaatc tgtattttta
     2881 taaattccat ggatattagt gcgttatatt ttttgacagg ttaataaacg tgatccaccg
     2941 cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag cccgataatt
     3001 agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa accctaatcc
     3061 agacgaactg atgatcgaag tcgatctctg ctatgaactg gacccgtatg aattaaaact
     3121 ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc ccgcctctga
     3181 tgcgctgacg cctgcggatc gctatctcga actgttcgag catgttcagt cggcgaaaat
     3241 tttccccgac agtaaaacct ttcccgactg cgcacccaaa atggacccgc tggatatttt
     3301 aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt ttgttgaaaa
     3361 tcacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc aaaattccct
     3421 gaaagagcat atcgaccagt tgtggccggt gctaacccgc gaaccacagg atcacattcc
     3481 gtggtcttct ctgctggcgc tcccgcagtc atatattgtc ccgggcggcc gttttagcga
     3541 aacctactat tgggattcct atttcaccat gctggggctg gcggaaagtg gtcgggaaga
     3601 tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactacg gtcacatccc
     3661 caacggcaac cgcacctatt atttgagccg ctcgcaacca ccggtttttg cgctgatggt
     3721 ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc acctgaaaat
     3781 ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg cctatcgcca
     3841 tgttgtgcgg atgccggacg gatcgctgct caaccgttac tgggacgatc gcgacacgcc
     3901 gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc gcccgcccaa
     3961 cgaggtgtac cgcgatttac gcgcgggggc ggcctccggt tgggattact cttcccgttg
     4021 gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc ccatcgatct
     4081 gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc tgaaaggtga
     4141 gaaagagaca gaagcactgt tccgccagaa ggccagtgcc cgtcgcgatg cggtaaaccg
     4201 ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac gcgaacaact
     4261 ggcgctgttt tccgctgccg ccattgtgcc actctatgtc ggtatggcga accatgaaca
     4321 ggccgatcgt ctggcaaacg ccgtgcgcag tcggttactg acacctggcg ggattctggc
     4381 aagcgagtac gaaaccggtg aacagtggga taaacccaac ggctgggcac cgttacagtg
     4441 gatggcaatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg aaatcgcgcg
     4501 cagctggctg aagacggtga atcagttcta tctggaacag cacaaactga tcgaaaaata
     4561 ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt tgcaggatgg
     4621 gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggcg aaccataata
     4681 tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctctta ggaggagata
     4741 tttaacatca ttgccgcctg ggtgcgattt ttcacttcca gacggcgata cagggattcc
     4801 agatgcgctt ttaccgttcc ggtactgata ttcagcgctc tgccgatctc cttatttgat
     4861 tcgcccgccg ctaacatggt taaaatctcc cgctggcggg cgcttaacga tttgagatct
     4921 ttaatgtcct tttccggcgt cgtccgccag tctccaggca gaaacatcat ccccatcgcc
     4981 gcactattta ccgccaacgc aaatgtctcg acggttgaat cacgaggcac aatggccagc
     5041 acattaaaat ggataacttc ctgtaaccac cgtttattgc aatccgtcgc cgtaattaac
     5101 accttaacct caggaaattg caccacggtt ttttgcagca accagtagca aaactcacca
     5161 tcctgatcgc catcgagcat aactaaggct tcagggtaat tttccagctt ttgccataac
     5221 tcgtctgcct gactggcccc ctgaatactc actcctggaa tacgctgctg taaactgatt
     5281 ttcattccat gaataaatat tgactgcctg tcaaacatga ctatttgcat aactgaatct
     5341 ccacctgaat acgttaaaaa gacttaagta gtggaagggt attacccgcg agaaaaaata
     5401 agaattcgcc atttggcggt ggccattcta cagagatgac gcgtagaaaa tagttaccga
     5461 tataaatagt tacagctaaa cgcctgaaat tacatgtcga gggcactatt taaaacaatt
     5521 ctgaggattt ccttatattg atggttagta cgcatgcaat taaaaatgaa attccgcgac
     5581 cacaagccaa aataacaaac ggcaatgaga caaaaataag cacaaatagc caatacgtcc
     5641 tctattcact ttaaagggaa tcgctgaaaa atacgctctg tttaagggga ttcacctttc
     5701 tcagaaagct attccgccct tttcctgctg agaaatcgcc acattcgtca tgacaacatt
     5761 gtgaaacccg gcattagatg ttagaaaaaa caataaacaa tgcgatatgc gcgttactgt
     5821 ttcgctgtga acaacaatcg gtcaaagaaa tggataaaat tcacgcaatg cagttgttca
     5881 tcaaagtcgc ggagctggaa agtttttccc gcgcagcgga tttctttgct ttgccaaagg
     5941 gaagtgtttc gcgccagata caggcactgg aacatcaact tggcacccag cttctccagc
     6001 gcaccacgcg acgggtcaaa ctcacgccag aaggcatgac ctattatcaa cgggcaaaag
     6061 atgtgttgag taatctcaac gaactggacg gtctgtttca gcaggatgcc accagtatca
     6121 gcggtaaatt acgcatcgac atcccgccag gaatcgcgaa aagcctgtta ctgccgcgcc
     6181 tgtcggaatt tctctatctg catccgggaa ttgagctgga actgagtagc catgaccgtc
     6241 cggtagatat tcttcatgat ggttttgatt gcgtgatacg cactggcgcg ttaccggaag
     6301 atggcgttat cgcccgtccc ctcggcaaac tgaccatggt caactgtgcc agtccgcact
     6361 atctgacgcg ctttggttat ccgcaaagcc ccgatgatct gacttcacac gcaatagtgc
     6421 gttatacacc gcacctgggt gtacatccgt taggttttga ggttgccagc gttaatggcg
     6481 tccagtggtt taagtctggc ggcatgttga cggtaaacag tagcgaaaac tatctcgccg
     6541 ccggtcttgc cggtctgggg attattcaga tcccgcgcat tgccgtgcgc gaagccctgc
     6601 gtgccgggcg gcttattgaa gtgttgccag gctaccgtgc cgagccgctc tccctttcgc
     6661 tggtttatcc gcagcgtcgg gagctttccc ggcgtgtaaa cctgtttatg cagtggctgg
     6721 ctggcgcaat gaaagagtac ctggactgac cgactatact ttttaagaat gaccacgaca
     6781 gaaggacaaa agagcggatg acgcaggaaa acgagatcaa acgtcccacc caggatctgg
     6841 agcacaagcc gattaagccg ttggataata gcgagaaagg cggcaaagtt agccaggcgc
     6901 tggaaaccgt caccaccact gccgaaaaag tccaacgcca accgattatt gcgcacctta
     6961 ttcgcgcgac agaacgcttt aacgatcggc tgggtaacca gtttggtgcg gctatcacct
     7021 atttctcatt tttgtcgatg atcccgattt tgatggtgtc gtttgccgca gggggctttg
     7081 tgctggcctc ccatccgatg ttgctacagg atatcttcga caaaattctg caaaacatca
     7141 gcgatccgac gctagccgct acgttgaaaa acaccatcaa caccgccgtt cagcagcgta
     7201 cgactgtagg gcttgtcggc ctggcggtgg cactttattc cggcatcaac tggatgggta
     7261 acctgcgtga agcgattcgt gcccagtcgc gcgatgtctg ggaacgctcg ccgcaagatc
     7321 aggagaagtt ctgggtaaaa tatctgcgtg attttatttc gctgattggt ttgttgattg
     7381 cgctgattgt gacgctttcg atcacctcgg ttgccggttc ggcgcagcaa atgattatta
     7441 gcgccctgca cctgaacagc attgagtggc tgaaaccgac gtggcggttg attggcctgg
     7501 cgatttccat cttcgccaac tatctgcttt tcttctggat cttctggcgg ttgccgcgcc
     7561 accgtccgcg caaaaaagcc ctgattcgcg gaacatttct ggctgctatt ggttttgaag
     7621 tgattaaaat cgtgatgacc tacaccctgc catcgctgat gaaatccccc tctggcgcag
     7681 catttggttc cgtgctgggg ctgatggcat ttttctactt cttcgcccgt ttgacgctgt
     7741 tttgcgcggc gtggattgcc accgccgaat ataaagacga cccgcgaatg ccggggaaaa
     7801 cgcagcctta aaataggccg gatgcggcgt acgaatttat tcggcctggc tccccgtagg
     7861 ccggataaga tgcgccagca tcgcatccgg ctataatgcg cacataacct cttgaaactc
     7921 ttccccagag cctcttcagc catctatttg ggagcaaaca atttcatgcc aactcataac
     7981 cccagcatat aaatccagtt ggtaactttt atttaacctg aaaccagttt tatccaccat
     8041 ttataaaatt atgtgaagca tttcatagaa gaaaaatcac tggcctaaac attatcccct
     8101 ttttgcctta tttttgacca tttccgcgat ttgttacaca ttgaaatatc acttttgctg
     8161 tgcgtaatat ggctattcgt tagccaaaaa ataagaaaag attatgcaag caacagccac
     8221 aacactcgac cacgagcaag aatacacgcc gatcaactcg cgtaataaag tccttgtcgc
     8281 ctctctcatt ggcacggcca ttgagttctt cgacttttac atttacgcca ctgcggccgt
     8341 tattgtattt ccgcatatct tcttcccgca gggcgatcct acggcagcaa cgctacagtc
     8401 gctcgccacc ttcgccatcg ccttcgtcgc gcgccccatt ggctctgccg tttttggtca
     8461 ttttggcgat cgcgttgggc gtaaagcgac gctggtcgcc tcgttgctaa cgatggggat
     8521 ttcgaccgtg gtgattggtc tgctgccggg ctatgccacg attggtattt tcgccccgct
     8581 gctgctggcg ctggctcgat ttggtcaggg tctgggctta ggcggtgaat ggggcggcgc
     8641 ggcgctgctg gcgactgaaa acgccccacc gcgcaaacgt gcactgtatg gctccttccc
     8701 gcagctgggc gcaccgattg gcttcttctt tgccaacggc actttcttgc tgctttcctg
     8761 gctactgacc gatgagcagt ttatgagctg gggctggcgt gtgccattta tcttctcggc
     8821 ggtgctggtc attatcggcc tgtatgttcg cgtgtcgctg catgagtcgc cggtgtttga
     8881 gaaagtcgct aaagcgaaaa aacaggtgaa gatcccgctg ggtacgctgc tgaccaaaca
     8941 tgttcgcgta accgtactgg gtacgttcat tatgctggca acctatacgc tgttttacat
     9001 catgacggtc tactctatga ccttcagtac cgccgccgcg ccagttgggc ttggcctgcc
     9061 gcgtaacgaa gtgttgtgga tgctgatgat ggcagttatt ggttttggcg tgatggtgcc
     9121 agtcgccgga ttactggctg atgcctttgg tcgccgtaaa agcatggtaa tcatcaccac
     9181 gctgatcatc ctgttcgcgc tgttcgcctt taacccactg ctcggttctg gcaacccgat
     9241 tctggttttt gccttcctgc tgctggggtt aagtctgatg ggtctgacct tcgggccaat
     9301 gggtgcgctg ttaccagagc tgtttccgac agaagtgcgt tacaccggag catcgttctc
     9361 ttacaacgta gcgtcgattc tcggggcttc cgttgcgcca tatatcgcag cctggttgca
     9421 gactaactac gggttaggtg cggtggggtt atatctggcg gcgatggctg gcttgacgtt
     9481 aatcgccctg ctgctgaccc atgagacgcg acatcagtcg ttgtaataag agtgaaaagc
     9541 ctgatgcgct acgcttatca ggcctgaatt tccatacaat atattgaatt cgcaaggatt
     9601 tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caaagcacac tttgccaaca
     9661 atctaaatct gtttaattgc cggatgtcag acatccggca attatcttca cttcttcatc
     9721 tgcgacaaaa tcgtccggca ctgattagcc tccccttcgg aaggtgagat cagtgccagc
     9781 aacgctgccg ctggcgtaac cagcgttgcc agtgccgcag caacagcacc acgggcaatc
     9841 aacggtccgg ctttcacccc agcctgcgga tttttaaatg tcccacgcac atacagcggc
     9901 gaacgcagtg tgataatccg aatcccttta ctctccggat caatagtcaa atccagctgt
     9961 tccgaagcaa aacttgccgt gccggtaacg ttaatcaacg cgttctcagt atcgaaagca
    10021 aaaatctgcg ggcgcgccac gccgttggca atattcagat tcgccgccgc gcagttcacc
    10081 cgcacctcat cgtcaccaaa tatcgcaccg acaatgtagt tgccgacatt cagcccaaca
    10141 atctccatca ggttgcggct caccagcccg tcattcatca acagtttcag gttgccgtta
    10201 ctgttgccta aaagtgccgc caccgagtta ccgctaccgc gcagttccgc gtcaccgttc
    10261 atttccccca gcgtcttctg catcagttcc acatcgggca tcagttcttt cagtttcagt
    10321 cgacgagcct gaatatctgc ccgcccctgc atcggctttt tatcgccttc cagatgaata
    10381 ttcgccgcaa tgctgccacc cgccatgcca aatttcagcg gttgcaggcg caggtcagca
    10441 tttttgagga tgatatgagt agaaagatcg ctaatcggca ggctactgcc atgctcgata
    10501 cgtcggcctt tgaaacgaac atcggcatcc atgacgtccc atttatcggt ttcgaagcgg
    10561 tcataaggca gcactttacc cgcaggctga acgctttttt cgcccttctt ctgttcagac
    10621 cgtttcgact tttcagcccc tttcccggaa tcaacgccaa tcaacggtcc caggtccgcc
    10681 agccgcaatt gccgcgactc gacatcacct tccagttttg gtcgtggctt tccggtggtg
    10741 tagaccagag aaccgtggat atcgctatcg ccaattcgcc cattaaaacc gcgataatca
    10801 aagaccgacg atttttcagt gtcgattttc gctaccagcc gaccatccgt ttcaaacggc
    10861 ggggtatcgg gcagcagaac gcccgtcagt tcatagagat cacccagtga atcgccagaa
    10921 aatttaagcc gtaaatcgac accgcccatc ttcattgggt cattcacgac gccatcaaaa
    10981 gcaacacggg tattacctga acggaaatca gcctgtaccg gaaacggcgt cccttcgcca
    11041 cgcagcgcca gcataccgcc tatttttccc gtaccagtga gcggttcacc gttataacgt
    11101 ccctgcgcct tcaggccaaa aacgtaatcg cccacctttt ctttatccgc tttacctttc
    11161 gatccagtaa cttcgctgaa cggcagtggc ttgcctaacg gatcgacaaa aatttccaga
    11221 tccgctttgc ttactttgtc atcaatggcg atccgccctt gatcgaaaag aatattatcc
    11281 agccgaaacg accatgccga cggctttgca ttcgcgtctt tgttgtcatc gttggcaaga
    11341 ttaaacgtcc agttattgtt cttttcagag aggcgaatca ggcgtgcgtc gggcttttcc
    11401 agtttgatcc acggtagcca gaccgttttg gtcagcagcg ccaacggtgc cagcgttgcc
    11461 tctacgcgtg gcaaatgcac catcgtgact tcgggaatat ccggtggatt gccaagaatg
    11521 atgtcttccg catgtacatg gggccacggc acccagctgc gccagccagt ttcttgtttt
    11581 tgccgctccc acaccacgcc cagatcgcca cggatagcga acggacgatt caactccgca
    11641 gagactttct ggttgatggt cggtttgagt cgattccagt caaaagttgc aatcaaaatg
    11701 atcgccacga caatcaacaa caagaaagcc cctgaaatcg cagcggttat tttgcctgcc
    11761 ttgctcatcg ttcgcttcct catattcttc ctgtgccagt cctaaagata gtccagccag
    11821 gcggaaaatg aggcagctta tagcgccaga accgccgtat tcagcgtttc tatcggtgcc
    11881 gggcgtgaaa gaaaccagcc ttgtgcggcg aatgcgggcg agttctgaac atcacgccac
    11941 tcttccggcg tttctacgcc ctcaacaatc accccgcgac aatagcgatt cattagatgt
    12001 aaaagctgag aaaagagtgt gcggccttcc gacgactgac gcagcatcac aaacagttct
    12061 cgcgcgattt tgatgtagtc ataacgcact tcacttagtg cagagaaatt tgccatcccg
    12121 gtaccaaaat catccagcca cagcgggcca aattcacaca tcgaggcaaa ggttgaatct
    12181 ttcggcagac ggatatgctc caccagttcg aaacgcagcc agggaagacg ctcaatctgg
    12241 cgcaggattt ttggttgctg acgcagggcg atgagcgtag ggccatcaat attgaccgat
    12301 gccagcaggc cgtgctctat aaagaagtcg gctttttgcg ccagcaaatc aatctgctct
    12361 ttcacaacct ccatccgatg gctgacggtg atttcagtaa aatagcgatc cggcggcagg
    12421 cgttgcgaag ggttcaaggg atgcgtgacc accgttaata gctccacggc cattaaccgc
    12481 ccgcatgttt gatagatcgg ctgccaggtg taagcacgct cacactgcaa ccaaaaacgc
    12541 cgttcctgca agctctcgat gcttgcttca gggttgctta ttcgctggat aacctgcctt
    12601 atcatcaaag atgtcctgtg tttaaaagtg atgctgcccg gactcgacaa agattatcgg
    12661 cgcgcaagga cagaacttta ccgcctggcc ggggtaaatt tcccctctcc acaacggaat
    12721 ctttcaggaa tacgttctgg ctcaaaaaaa taatggaaca ctgttttaat atggttgacc
    12781 agcaaacaac cacagcgcaa actaacgcta attttttaca gatcaggttc acgactatgt
    12841 ccaaaaagat tgccgtgatt ggcgaatgca tgattgagct ttccgagaaa ggcgcggacg
    12901 ttaagcgcgg tttcggcggc gataccctga acacttccgt ctatatcgcc cgtcaggtcg
    12961 atcctgcggc attaaccgtt cattacgtaa cggcgctggg aacggacagt tttagccagc
    13021 agatgctgga cgcctggcac ggcgagaacg ttgatacttc cctgacccaa cggatggaaa
    13081 accgtcttcc gggcctttac tacattgaaa ccgacagcac cggtgaacgt acgttctact
    13141 actggcgtaa cgaagccgct gccaaattct ggctggagag cgagcagtct gcggcgattt
    13201 gtgcagagct ggcgaatttc gattatctct acctgagcgg gattagcctg gcgatcttaa
    13261 gcccgaccag ccgcgaaaag ctgctttccc tgctgcgcga atgccgcgcc aacggcggaa
    13321 aagtgatttt cgacaataac tatcgtccgc gcctgtgggc cagcaaagaa gagacacagc
    13381 aggtgtacca acaaatgctg gaatgcacgg atatcgcctt cctgacgctg gacgatgaag
    13441 acgcgctgtg gggtcaacag ccggtggaag acgtcattgc gcgcacccat aacgcgggcg
    13501 tgaaagaagt ggtggtgaaa cgcggggcgg attcttgcct ggtgtccatt gctggcgaag
    13561 ggttagtgga tgttccagcg gtgaaactgc cgaaagaaaa agtgatcgat accaccgcag
    13621 ctggcgactc tttcagtgcc ggttatctgg cggtacgttt gacgggcggc agtgcggaaa
    13681 acgcagcgaa acgcgggcac ctgaccgcaa gtaccgttat tcagtatcgc ggcgcgatta
    13741 tcccgcgtga ggcgatgcca gcgtaaacgc agcatatgca aaaggctgga taaagtgtta
    13801 cccctctatc cagctcatcc ataaatacct gatggttgtg ctaactcatc accatcaggc
    13861 attactgtgc aggtggaata tccgtcactt caggatgtac gtcatccgtg gcgacagagg
    13921 tggtcgcagc ggcggtagat ggggccatga tttgatccca ggccgcctgc aacgctttca
    13981 tgttaaattc cggctcgcct ttcggctgca gcagtattaa cgccatatca ttcgacaact
    14041 gctgacgcag atcctgattt aacatctcca cagtcaggct gttaaggaaa tcctgccgca
    14101 gtttctgata ctgctccggc gcgatatcga cgacctgatt ttgcaacgaa cgcatccgct
    14161 gacccatcag aatatcggta tcagctcgtg cataggcggc aaacagtttc tgcagctcca
    14221 gtttcttttg cgccactaac gcattgaact cttcttccgg cagaccttta tcgcgaacct
    14281 tcgccagttc acgcgccacc agattaaggt tgctgttcag cttgtcgttt ggtgattcga
    14341 tgttgatggc acactgcgca cgcagataca gcacacggca gtcaaatcca agaccgatgt
    14401 ctttgctgtt actggcgctt aacgcttgct gaacatgcca gaacagcgcc tcacgggcca
    14461 ggtccgcacg ccagtagcgc agcagtgcgg ctgattcacg aatcggctgc cacggcgtat
    14521 cccacatgat agataaccgg tcctgacgca ccgcgtcagt cataatactc accgcttccg
    14581 cacgcagcgg agaaagcgtc ggcaccggag ccggtgtttc acgtttgcct ttcagttcgc
    14641 caaacgtttt gttgatttgg tcgacaaccg agcgcgcatc cacgtttccc accaccagca
    14701 gcgtcattgc atccggggtg taccatttct gatagaaatc tttaattttt tccgcttcaa
    14761 cgggttgttt cagcggatcg gcaggatcgt gacctaacaa ggttgatcct ttcagacgat
    14821 agcgccacca gccctcttta gtatcggcag gccaggttgc caccatgtcc tgactttgca
    14881 gcgcgtggtt gatggtttct ggtgtgatgg tcagtttgcc agtggcattt gccagataag
    14941 agagcgcctc tttcagcaaa tcgttacggt tattgggcaa actcagatta aacagcgtgg
    15001 tgtcataaga gacaattacc ggcggcatcg ggcgtttagg gtcgatcccc tgctgccaca
    15061 atgaacgcgc ctgtgctgcg tcaaggccac cgctttgcgt tagcgcaata cgagggatgg
    15121 cgtgactgta accgctctgt tgtgtacttt cggcgagcga accggtatta accagcaggc
    15181 gaatttcaac acgatcgctg ggacgctggg gggtggtcag cacttgccac tgtaaaccgt
    15241 tggaaagcgt cccctgttgc catgctggat caggctggag cgcatctgcc tgcacatagc
    15301 cagcagtggc catcatcagc aaaccgcccg ctaaaagtcg aatttttgtg ccctgcatgt
    15361 gaacccctga tcaactatcc tggtaataaa aaaactgccc gttgtcggtc agtctaaatg
    15421 acgttaaaaa cacttcgtgt tagaccgcaa gaacatgaaa atgtcacgga agaagtgaaa
    15481 taaacctgaa ctcacccagg tggtaagttc aggcacaggg gtcaattatg cgcaaacacc
    15541 cgcactcggg gaagggagtg cgggcataag tgatgagatt aagaggataa ttcgtgcgtt
    15601 ttgccatccg gcgcacgatt attcagcaca tcgtccagtt ttttgtggtc cagttctttc
    15661 acccacttag caacgacaat ggtcgctacg ccgttaccga ccaggttagt cagcgcacga
    15721 gcttctgaca taaagcggtc gataccgagg atcagcgcca gacccgctac cggcaaatgg
    15781 cccaccgcag agagcgtcgc cgccagcacg ataaagccac tacccgttac ccctgccgcc
    15841 cctttagaag aaagcagcaa cacgattaac agcgtgattt ggtggacgat atccatctga
    15901 ctgttagtgg cctgggcgat aaacaccgcc gccattgtca ggtatatcga tgtgccatca
    15961 aggttaaacg agtagcctgt cgggatgacc agccccacca ccgatttacg gcagccgagt
    16021 ttctccatct tgtcgagcat acgcggcagc gccgactcgg aagatgaagt ccccagtaca
    16081 atcagcagtt cttcacggat gtagcggata aatttgaaga tactgaaacc agtcgcttta
    16141 gcgattgaac ccaataccag caccacaaac aggatacagg taatgtagaa acagataatc
    16201 agctgcccca gttgcaccag tgtgccgacg ccgtatttac cgatggtaaa cgccattgcc
    16261 ccgaacgcac caataggtgc cagacgcatg atcatattga tgatgccgaa gatgacctgc
    16321 gagaaacttt cgatgacgtt aaaaatcagt tggcctttgc tgcccagacg gtggagcgca
    16381 aaaccaaaca gtacggcaaa cagcagcacc tgcagaatgt taccgctggc aaatgcgcca
    16441 atgacgctcg ccgggatgac atccataatg aaggcgacaa tgccctggtc tttcgcctga
    16501 tcggcgtaaa ccgctaccgc tttcgcatca agcgttgccg gatcgacgtt cattccggca
    16561 ccaggctgca cgacgttaac gatgataaga ccaataatca gcgcgatggt actgacaatt
    16621 tcaaagtaaa gcagtgcgac tgcgccggta cgaccgaccg ccttcatgct ttccatgccc
    16681 gcgatgcccg ttacgacggt acaaaagatg acaggagcga tgatcatctt aatgagctta
    16741 acgaagccgt cgccaagcgg tttcatttgc tcgcctattt caggatagaa atggccaagg
    16801 agaataccaa tggctatcgc tgtcaggacc tgaaagtaaa ggcttttaaa cagagaggtt
    16861 ttcatagggt gtcctttagt aaaaccacag gtcttgtaag gttatggggt acctgcggcc
    16921 ttaaaataac acccagacaa catcacagaa atgtacctgg atcataattg aaacaaaaag
    16981 gttaaaaagt ttgagctggc tcgcacaaac cagcactttt taaagttttg taatcagttt
    17041 ggggtagcta cttttcttcc aggtaactct cttcgaagat ttcaataggg agtgggcgag
    17101 caaaaaggaa gccctgggca ataccaacgc ccgcttttgc cagccagtcg cgttgtgctt
    17161 cagtctccac gccttcggca atcatttgta agttcaggct ctgcgccagc atgataattg
    17221 cagcaatcat gctgctatct tccggcaagc cttcaacaaa cattttgtcg attttcagaa
    17281 cgtcgattgg caacgatttc atatgctgca gctgacgcag ccctgcgtag cccatgccga
    17341 aatcatccag cgccacccga actccggcat tgcgcagcgg acggaggatt gccaccgcag
    17401 catgagggtc gtcaatacgt cggctttctg tcacttccag aatcagtgtt cccggctgaa
    17461 tgcgatagcg ggttaacagt tccagcatat ccgccaccat attcgggtgc atcagttgca
    17521 gcgcagagag gtttaccgac aagggcagca taatgccgcg ctcttgccag gcagccaaca
    17581 gtcgacagga ctcttccagc acccagtgac cgacggtaac catcagccca cagcactcaa
    17641 tgcgatcgat taagccatcc ggcaggtccc aactgccatc cggttgctgg atacgcagta
    17701 acacttccgc actgaccagt ttaccgctgg tcatctcgac ctgtggctgt aaccaaatag
    17761 caaactgatg attttccagt gcattaagga tatcgctctc ttccgtcaac cgcttctggg
    17821 cggcttccat ctgctgcgga tcaaagaact gaatctgatt cttgccttta tggcgagcgg
    17881 taaatgccgc agaaatagcg cgactgtaaa gctgttcggc ggtgagatcg ccgtagaaca
    17941 tcgccacgcc aatgctacag tgcggacgga gttgaatacg ttcaatcggc aggcgctcgc
    18001 tcatgatagt gagcacttgc tgacctaagg tgattgcgtg ccacggttcc tgtacaccat
    18061 tggcaatgac agcaaagtca taaccgctaa tctgcgcgag gatcatacgt ggcgacagta
    18121 ccgatttgag tttttccacc agcgtcagca gcagaatttc tcgttgcgcc tctttcagca
    18181 cacccgcagt atcacgcagg gtttcacagg tgatgatcat cagcgcggtg gtttgtttac
    18241 gcgcgacaac ctgctccagc atctccatca gcaaggcttt gttcggcaaa tccgacactg
    18301 ggaagcgcat cgcattttcg ttctgttctt cataatggcg ctgcagcaat tgctggttga
    18361 ggttgtaact gcgcaccaac ataccgattt catcatcctg atgcagacgc ggtaatgcca
    18421 gttggtgacc aacaagctcc tgggctggga tggcgttaag ttcgcgagca atattgcgta
    18481 acggatgcaa aatcaggcgg ttaatgcacc agctaatcgc caccgtcaat attaacgaca
    18541 aaagtaagta aatggtcact aacgttgaga gggtgctcat cacgaactta tacatacgga
    18601 aggaatccgc ctgtagcacc agataggcaa ttggctgcgg atttgccgga cgttcgagcg
    18661 agtagacgcc cagcgagatt tgaaccggta gctcaaacag gcgagtaacc attaccggca
    18721 ccgggcgctc tggaataaaa cttttacgca gcgcctggaa ctggttgggc agcactacat
    18781 cggcacggct gaccacgcct gccggtttaa tgctggcaag aatagcttcc gcttcgggaa
    18841 tatcgccttt caaaatggct gaagataaag gttcgcggac agagcgagca atgctttcca
    18901 gttgcgtagc cgtgttatag cgattctgct ggaccagatg gaacagcaaa acggtgcaaa
    18961 aaataaaaac gaacaccagg acaacggctg ccaccattgc catctgcttg attgttaacg
    19021 agcgacttac gcgcaaattg tctccccccg gaaatcccag attgccgccc gagtataccc
    19081 gatcgcagcg gcattaagag aggcgctatc tgaaaactta ccagtcggcg taaggtatca
    19141 gcggctgcgg cggtaaatcc atgtcaccct gccatccggc ggcggaataa cgtacgtaga
    19201 gcaggaaatg gctgggtgcg taatctttcg cctgctggat atcaattgcc gtgccgacaa
    19261 accagttgga tgtgacgcga cgctcaagta atgcccgcgc cgtatagcca aagccctgac
    19321 tgctgccgcc atcgttggat tgccgcgcag cttcttcctg ccagtcagtc gggatcagat
    19381 tcatcagcgg ataacgcggc atggttttgg tgcgtgaatg cgaccacgag ccagacgccc
    19441 ccagctccca cgaccagttt tccgtgcgct cccgccacat caccggtatg gcaaacgaca
    19501 ggtattcctg cgggctgtag taaccgccct gaccgagtga gtagccactc aggtctttgt
    19561 cgtaatgcca gatcatgttg ttcaggccga ttgtgacgcg gcgattgttc tggttgatga
    19621 ccttatagta atagcccgtc atccagcgca cgcgccagtt atcttcgaca tttttaccgg
    19681 ttaactggtc gccactaagc gatgcccaga cgccgtttgc ttcaccttta tcgtagctca
    19741 ggcttagccc cacgccgtcg gcacgcacac cgccccattt tttcccggta ttgctcgggg
    19801 agtctttttg cccaccaaag gccagcaaag aactggagat gggccgacgg tgggcgttaa
    19861 cggtgtaacc cagcggcccg atatcatcgc tgtaactgat gccgccgacc acatccacca
    19921 cgttgaagcc catcggcgtg gtgccgatat cccagctcca gacgtcattt cgccagccga
    19981 ccgccacgct ggcaccggaa tccgactggc tgcggttgcc gctacagtcc tgtaatgtac
    20041 aggtgcccca gttatcatcc catttaccat cggcattagt ggagaaactg ccgacgttca
    20101 tattgacgaa atcactgcga aagaacatcc gcccgtcaga atacggcgca tccacctgca
    20161 acatggtagt gtgcgctttc agatcggagt aaccaccggt gccgctcgaa ccccaataat
    20221 catgctcgag ggtgacgtta agatcctgct ggcgatagag gtccgccgca tcgctgcgca
    20281 cgccgcgttt cagccagtca tctttctcgt cgttacgggt cagtcgggta aaggtgtcgt
    20341 tatcctgcgg acgcgtcgta gtcacaccgg atgcgaccat ggcgtctttg taggtttcca
    20401 gcgcctgctt tggatcgccc gcctgcgctt caaacttcgc accatcacgc agtaccatcg
    20461 cactttccat cgacggtggc tgagattttg cctgcgggat caccttatta aacgtctgct
    20521 gcgccgctgc ggtatcgcca agctgcgcct gcgccagcgc cacgcgccgc tgtgtgttca
    20581 gcgaggcgtt atcggtggca ggcagtttcg ccagctggct acgtgccgcc gatttgtcac
    20641 cagcagcaat atccacttcc gtcagaccaa gaatggcatc ggcgttagtt ggctcccgcg
    20701 tcaggacatt ctgatatgca gcgcgggcgg cggtgtaatc acgtcgctgc tgcgcccagt
    20761 cagccagcgt gaggtcaata cgcgtggaag gtggttgctg acgcagcatc gcttccgctt
    20821 cagcttcttt gccgctttct cgcaggcgat tagcggtttc cagcacctga tcgctttgca
    20881 gtcgattaac cagctcctga atattgctgc tccactgcgc acgcggcagg ctgttgatat
    20941 gcgccagcgc cgctctgtcc tggtcatgac cagagaggta cagcccgtaa gcgtaaacct
    21001 gctccgggtc gttcggcttc tgctgcgcca gattgcgcat taacgtatcg gcctggctgc
    21061 gttgtccggc ctgccagaga tcctgcgaaa gtcggtaagt aatccatacg ctgccggggt
    21121 ccagcgccag tcgttgccgc tgaagtgctg ccgcctgcgc ccatttgccc tggttttcca
    21181 gtgcctctgc ctgctgtgcc agacggtcgt tttgcaggct gcgttcgata tcatcaatgc
    21241 tacgtcgctg actggcagag agcgaggcga taaacgcttc agctttttct ggcgattgct
    21301 ggcggtaaat atttgccagc ccgcgcacgg cgttagtgtt gccgctgtcc atacgcaagg
    21361 tcagctgata ataacgttcg gcggcgggat aatcttttcg cgccatcgcc acatcgccca
    21421 gccccagcac tgcataactg tcggtgttat cgacattacg cgcctgctgg aacaggcgtt
    21481 ctgcccggtc aggattattg gctttcagcg cagcatcgcc ctgctggatt gccagccagt
    21541 aacggtttac tttcagcaga ctgttccatt tgtcgttgtt gctgctgtgc ggatccagtg
    21601 cgagggcttt ttccagattc gccactgcat tggcgcgatc gcctttctgt gaatatgcct
    21661 ggcccagcgc ccccagagcc tcactgtctt tcgggttcgc ccgcactgcc tgttgtagtt
    21721 cgggaatggc tttacccgcc ataccagagt ccaccgccgc taaaccttgc gcacgagcgc
    21781 ggaaagcagg atcggccagc tgtttttgct gttctgccag ttgcgattgc gcagccgcca
    21841 cgctatcgcc atcactaaag atcgaaagat attttttcag cgcctgcacg ctggcatcgc
    21901 tgacgggcat gtctttaatc tgcccgtacc agattttaga ggccccttcg cgcccggcgt
    21961 tcgatttcgc catctgttcc aggacggcaa aaccttcgtc acggcgatcg ctactaaaca
    22021 gcaatagcgc cagattgttt tgcaggcccg tattgcccgg tgcatccgca ttgatgcgtt
    22081 ttaactgatt aatcgcttcg ccacggcgag ccggaatttt cgccaccgta ctccagtact
    22141 cgacagcaat gtcaccttcc ggcggcgcac cgttgaacag cttgttgtaa ctcgccacag
    22201 cttcttccgc atgaccggta gtcgcctgca atcttgcctg ttgcagtgcc tgacgaccat
    22261 ctggcgtgga aagtagcatc gtagtccgcg acgatttata cgcatttgaa ctcggcgcta
    22321 actgcgacag ccgatcgagc tgtttttgcg cgccatcaat atcgccctga cgtaacaaag
    22381 aacggaaacg ggcggcaacg acgtccggat tattcggatc aataagttcc agccgataca
    22441 acgactgttg caccaggtct tcacgatggg tcgcttcgcc taaccgaact tgctccagca
    22501 actgttgctg agcggttggt gctgcctcga ccatcggcat gacggccaga ccgagggaaa
    22561 gcgtgaatat gtttagtgtg aatttgcgca ttcctggccc cagtcaggta ataactcacc
    22621 ttttgtcgag aagcggaaac ggtgttgatc ccagccttgt ccaaacaggg tcagcacata
    22681 gttgtaatag gcatcgctgc cgggaaagtt atcggccacg cgctggcgct gaacggcctg
    22741 cgcatcacgg ttttgtaaaa agggcagcat ggcggcagaa aaaccgaccg gtcctttacc
    22801 ctgcgctttc cccgtagcca catccacttt ttccggcgga taaccgtttt tctcagtgaa
    22861 tgtcgccatc ggtttaaacc ggttgagcat ccgcgctttc tgcggatcgc tgtcaggcat
    22921 catgcctacc cacatgtaaa cgcggatagc gtcgtagctg ctgatcaatg ttttttcggc
    22981 ttttagctgc cagcctttgt ctttctcata gcgcacccag tctggcgaaa agcctttcgg
    23041 ggcggtttcc agcaataaac gttgattggt ttcgcgcagc gtggtccacg gcgcgccaaa
    23101 gcgggtgaaa tactgcgcca gcgtcggcgg caggtagctg gggttaaaac gccagctgtt
    23161 atcctctgca aaacccactt tgcccggtaa caacatggaa cccagcccag gcaccgtcac
    23221 cacttcctcc cgcgcaatac gttttagcaa cgcgctgccg atgtcggtat aacgctgctc
    23281 tttccacaaa cgccccgcct ccagcaacga ccaggccatc cagacatcac catcggaggc
    23341 cgaattgctg tccagcactt cccacttact gttctctttc ttgccccaca gccaggcggg
    23401 caaacgttct tttaaagaac cctgagcgag attgttctgc gtccagtcga gaatattatc
    23461 gaaagctgca cggtcgttag ccgccagggc aaagaacatg ccgtaacttt gcccttcaga
    23521 ggtggtgatt ttgcgcgcgt cgctggggtc gatgacgcgc ccttcctgac tgatgtaatc
    23581 ctttttaaac tgctcccagg caggccaggt acaggctgcc tgaacactaa aggcagccaa
    23641 cagcagcatc gtcacgattc cactacgcaa cacattcatc ttcaattact cgttatccgg
    23701 gttaagacga cgacgactaa taatacgcag cagacgccac agtacccatg ccagcaatat
    23761 cacactgata gccgccagca ccgccagcag aatcggatgg tttgccagcg cataccacaa
    23821 gcgctcgaac cacggcagat gacctacgta ataaacgtcg ccaacacgta ggctgttgat
    23881 accggactcg cggatcaccg cgaccgaacc gaacatggtg gcgcgtttgc cgctatcgtt
    23941 caccgcattg ttaagcattt cataaccgcg tgggctatct gccagcagcg caatcacgct
    24001 gcgctggtcg ttatacggcg actggaagcc aatcaccgcc gccatcgcac cggaagaggt
    24061 cagcgttgac tgggtttctg ccgcgcgatc gctctcgtcc ggcacaatgc cggggaacgg
    24121 ggtctggcgc atcggtgttt tcacccagct ttcggtcgcc tgcaccaata ggtcgatctg
    24181 cttatcgtct ttcagtttgt ccgggatacc accgatgatc atgatgtcgg catctttgcc
    24241 ctgaatggtg ctgccatcat cggtcaccgt cagattaatc gccgggaagc ccgtctgtgc
    24301 gccgataaaa ccaacagtat tcagcaacgt ttccatctgt gcttcgttag gcgttttcgg
    24361 catcacggtg atggtttgcg acagatccgc catccggctg aatgggaagc ccgcgttagc
    24421 aaaggcgcgt agatccggca tcgggatgaa gtggtaatac ttcgagaagt cgatggtgga
    24481 gtcgtcacca atcaccacat gattctgcac cggctggaag gtaatacagt tatccaccga
    24541 accgccaggc atcgggttca tatactcaaa gtcgaagcgc agctggttgg tcgcgcccag
    24601 tttcagcgcc ggaatagaga catccgtttt tccatccagc aaaccttgta ataccggaat
    24661 ccgcagcagc aggcgattcg cctcctgttt gctgctcagg ttgaaggatt gcaggaactg
    24721 gttattcagg ctgatatcca tccgcgaact gtctttcacc ggcggcatgg tgtaacggta
    24781 attaatatcc atatcaatgc cggtactgcg catcaggtag agatccggtg gcaggtttag
    24841 cgaaacgtta atcgctgctg gctcaagacc gctggattgt aactgttctt cataggtttt
    24901 cagttcacca aaggtgaccg gacgatcggt acgcacccag ttcggcgcat cgtacggctt
    24961 acgcggtagc agcggtttca cttcattcac taccacgctt tcaccacgga acagtatgtt
    25021 accctgagcg atacctttcg ctgcctgcaa caggtctttg tcgtcacgac caaacaccac
    25081 cagcagtttg acgtaaggat tctgcggatg gttaatcatc tcaatcaccg gggcttttac
    25141 cgccggatga tcgcgcagga agtccggccg tttgtcgttg gttgcaaaga caatggcatt
    25201 acgatccggc agttggttat agagcaccgg gaagttctgc ccacgccagc cagaacgcga
    25261 accaaaccac gaggcgacaa tggcagaggc ttgttgcagc ccaacatccg gcgcacccgc
    25321 aaagaccatc ggcaaggtgt tagtacggtt atcgcgcggg tcaaagaatg gcaccgggaa
    25381 gtgtgacagg tcattcttca cattcagggt ctgataggtc agatccagtc cactgctccg
    25441 cccaacatcc agccaaagcg tggtgctggc cgggttttcg cacacgtcct gataatggcc
    25501 gacaaactcc agccgcacac ggttgaagtc ggtaataaac agtggattaa tgggcatttg
    25561 cgccagcgtt tttttaccca actgttcttt ggtcactggc agcacgccca tcagttcatc
    25621 attgagataa acctttaact gcgactggac aggcagtaac gatggcgatg gcgtgtattc
    25681 gaggttgagc atcgccttcg tcaccacttc atcgctgcgc ataccaaact caatgctgcc
    25741 gttcgggtta atgccacgta gcaccatgct gcccggcggc ggcgcaattt gcgcaaaggt
    25801 cagcttcaca tcacgcgaag gaccgttctg cgccacgact ggcgcatcag cgccctgcac
    25861 gcccggcatc acttgcccca cctgcggatt ttgttccgtc tgggcggcta tagctggctc
    25921 agcattgatc agtggttgcg ttgctggcgt cgcctgcgtc atgaaagagg ggaacgcact
    25981 catccccata gccactgcac aaatccagaa tagttttctt ttcatcgcgt tatcatcatt
    26041 gttgagccaa agcctgatcc gatggttgtg ccgtttcgct ccgctccggg cggcgcggaa
    26101 taaacgatac aacccaggaa accagagaag tcagcacacg gaatatgccc ttcaccgaag
    26161 aaggcgcaaa ctccgccaga tggcggtagc cacggaagcc gagcttcaga atatccagca
    26221 gactttccag cggcttatct tccggatagc tgtcctgcca gagcgcccat gtatccgcac
    26281 gggcaaacgt acactgcaca aaatcgatat gttgctgggt ggtgagcggc attaatttca
    26341 gcccaacttc attacccatc acgcgcgcca cctgggtcgg gaagacgtat tcctgctgac
    26401 cacgtttaag caacagattc actttctgcc cttccagaat ctgcgcctga ccgttgatct
    26461 tgatccccaa accaccgtcg gagaaatcct gaacggtaca cgaaaagagg tgaccatctt
    26521 cgcgggcaat tgccgcgggc atcgtcatct ccacgcggtg cgatcggcgt acctgtttgc
    26581 tttctaccga taccgcaact gcgccgccaa gaacaatcag gttgtagaat acccacacca
    26641 tactgacgac cacggtgagc atctcggttg gcgggccata gaagtagcgc cagatgccta
    26701 ccgcaacgcc caccaggttg agcaggacaa ggaagatgta gggccgcgag atcacccaat
    26761 cgacgtactc ttcttccacc agtccacctt tggcggtgac gttaaatttg cctttgtgcg
    26821 ggttaatcag cgccaccagc gtcggtggtg cgatatacca cgccagcacc gtttcgtaga
    26881 tttcactcca gaaagagtgg cgatatttgc cctggatctt ggagttggtc aggctggcat
    26941 ggatcatatg cggcagcacg aatagggcga tcatcaacgc tggcgcatag atgatgtagg
    27001 catgaagcag caggaacgcc agcggcgcag tcaggaagat cagccgtgga atgcccgaca
    27061 agaagtggaa catggcgttg acgtaacaca gccgctgagc aaacttcagc cctttaccgg
    27121 tgagcgggtt atcgagacgg aagatttgta ccatcccgcg cgcccagcga atacgctgac
    27181 cgatatgcgc cgacagactt tcggtcgcca gccccgccgc ctgcggaata cgcatatacg
    27241 cggaggtata gccacgacgg tgcaaacgca gagaagtatg cgcatcttca gtcacggttt
    27301 cgacagcaat gccgccaatt tcatccagcg gcttacgacg aatcaccgca caggaaccgc
    27361 agaagaaagt ggcgtcccac atatcgttgc catcctgcac cagaccatag aacagcgtgc
    27421 cttcgttcgg cgttttacgg aaacgcccca ggttgcgttc aaacgggtcc ggcgagaaga
    27481 agtggtgtgg agtctgcatc atcgccagct gtttctcttt caggaaccag cccatggtca
    27541 tttgcaggaa tgaacgtgtg gggacatggt cgcagtcgaa aatcgacacg aactcgcctt
    27601 tggcatattt cagcgcattg ttgatgttgc ccgctttcgc atgttcatga gtagtgcggg
    27661 cgatatattt cacccccacg ttttgcgcaa actggcgaaa ctcttccctg ccgccgtcat
    27721 cgaggatcca gatgttcagt ttgtctttcg gccagtcgat acccagcgag gcgtaaatgg
    27781 tatttttcac cacgttgaga tcttcgttgt aagtcgggac aaagatatcc accgacggcc
    27841 acagcgacat atctttcggc aatggcaccg gctgacgatt cagcggccat actacctgga
    27901 agtagccgag caccagcaca atccacgcgt acgtttcagc gaagagcaga ataagcccgc
    27961 acaccaggct gaccggatcg tcccagttca gcgtagaggt gtaacgccac cagatataac
    28021 ggcaagaaac ggtcagcgac agcacaatca acattagcgc cgagaagcgc cccggcatcc
    28081 gccgtacgat cagcgctacc ccccacagca gcatcaggaa gataaactgc gccagcgggt
    28141 taaacggctg agtaacgcag attaacgcca gaatcagcga gaaggtgacg atgataccga
    28201 ggatcaaccg ccgcgccccg gcactcaaat gaccgagctc ttttttctca tcaagatgct
    28261 gtgttttatg gctaacgcgc tcaggcagct cgttcatcca ttgatggtaa cgtccacgaa
    28321 tattctgcag acctgaaaat gcccgcctgc gcggtttcgg cgtttctttg cgcgatgcac
    28381 cgatcagtaa ccagcatgtt tgaatgagat aacggaccgg gtccagcgga cgcggacgcg
    28441 aggcgttgat atgcggatac aggtttttat gttctgcgcg aatacgctgc cagcgcgggt
    28501 gctccagcgg aataaaaatc caggccagga tcaaccaaaa acagccgagc gtcgcgctga
    28561 aagccgacgc accgtgacga cgataatcgc gataacgccc gataagccgc gcgttgaccg
    28621 gcgggattag caaccaccgg gtcaggatac tcatgattta ctcccgactg gcgttttcag
    28681 cccggaatag ttcaacaggc accagttcgc cagcgtcaat atctcttcag ccgccagcgc
    28741 atcactgcga tattcaccta ctggttgctt agccgccagg cattcagcca tcgcttcatc
    28801 acgatgaatg agcatcggca gtaatcggcg ctggctttgc aaccaaagct ggtaaatatc
    28861 gtcctgaacc tgactgccaa tacggaagtc attaatcaaa atatgtgcgc catccggcag
    28921 cgcttgctga tgcagtcgga tatggcagtt ggcatccaca ttgacgattg ccagcgagtg
    28981 atcgcacaaa ctcagcagct ggtgggttat ctgcgaggca tcacgcggta agtcgattaa
    29041 aatccactgg taacgcccgc tggattttag ttgctgtaag ccggagcaaa tatcgctcag
    29101 ccgggtttgc cagtgctgtg gattttcttg ttcttcaatg gataactgac caaaaggtag
    29161 caaatcgagc tgcgaggtgt agcgcaaccc agcgtcacgc cagtcctggc catccagcat
    29221 agctctggcc cagccctgac ggtgggtaaa atcaacgtta aatgacaggc gcaacaagtt
    29281 gtccgggcag gcatcgacca ccaggacatt ttctcccaac atttgtaatg accaggctaa
    29341 tgcggcggtg atggttgttg tccccacgcc tccccgcacc ccctgcaatc ccagtacggc
    29401 cattcaccaa tcccctactt ttgttgcgca aactctgcca gcaacggcca gcgttttaat
    29461 gccgcggcca actgttcgcg ttgggaaata tcggcataat caatatcagg cagtgaaaat
    29521 gcctgcttta acgccaaaat atcattctgg aagatatagc ctatcgcggg atcaggcaga
    29581 gtatctggtt cattgttatt cattttgctt gatccctatg aaataaccgt accgttcatg
    29641 agacaattct aaccgtgagc atttcgccgg tttattgttt acggcgagac gggaatttgc
    29701 tcactttttg actttccgtt acgcaacaga aaaagttaaa aacgaaaaga ctaaaatcac
    29761 acgtcttgtg acctgctaaa ttgattcaca tgctaaatct gataagtttt aatttcaatg
    29821 gtaggtttat ttcttagctt tcgctagtaa actgataatc agacaaaatg gtgacatgag
    29881 ggacattgtg gaccctgtat tctctatcgg tatctcatca ttatgggatg agctgcgaca
    29941 tatgccagca ggcggcgtct ggtggtttaa cgtcgatcgc catgaagatg ctatcagtct
    30001 ggcgaatcaa acaattgcat cccaggctga aaccgcacac gtcgcggtca ttagcatgga
    30061 cagcgatccg gcgaaaatct ttcaattaga tgattctcaa gggccggaaa aaataaaatt
    30121 attttcaatg ctaaatcatg aaaaaggtct atactatttg gcccgtgatt tgcagtgttc
    30181 tattgatccc cataattacc tttttattct tgtttgcgca aataacgcat ggcaaaacat
    30241 tcctgccgag cggctgcgct catggttgga taaaatgaat aaatggagca ggttaaacca
    30301 ttgttcgctt ttggtaatta atcccggaaa taataacgat aaacaatttt cattgttgct
    30361 tgaggaatac cgttcacttt ttggtcttgc cagtttgcgt tttcagggcg accaacattt
    30421 gctggatatt gccttctggt gcaacgaaaa aggggtcagc gcccgtcagc agcttagcgt
    30481 tcagcaacaa aatggtatct ggacattagt tcaaagcgaa gaggcggaga tccaaccacg
    30541 cagcgacgaa aaacgcattc tgagtaatgt tgctgtactg gaaggtgcgc cgccgctatc
    30601 ggaacactgg caactgttca acaataacga agtcctgttc aatgaagccc gtaccgctca
    30661 ggcggcgacg gtggtctttt ctttacagca aaatgcgcaa atcgagccac tggcccgcag
    30721 cattcatacc ctgcgtcgcc agcgcgggag tgcgatgaaa atcctcgtgc gggaaaatac
    30781 ccctagcctg cgcgccaccg atgaacgttt gttattggcc tgcggtgcaa atatggttat
    30841 tccgtggaat gcgccactct cccgttgtct gacgatgatc gaaagcgtgc aagggcagaa
    30901 gtttagtcgc tatgttccgg aagatatcac taccttgctg tcaatgaccc agccgctcaa
    30961 actgcgtggt ttccagaagt gggatgtgtt ctgtaatgcc gtcaacaaca tgatgaataa
    31021 ccctctcttg cctgcccacg gtaaaggcgt tctggttgcc ctacgtccgg taccgggtat
    31081 ccgcgttgag caagccctga cgctatgtcg ccctaaccgt accggcgata tcatgaccat
    31141 tggcggtaat cggctggtgc tgtttctctc attctgtcgg attaacgatc tggataccgc
    31201 gttgaatcat attttcccat tgcctactgg cgacattttc tcaaaccgta tggtctggtt
    31261 tgaagatgat caaatcagtg ccgagctggt gcagatgcgc ctgcttgccc cagaacaatg
    31321 gggcatgccg ctgcctttaa cgcaaagttc taaaccggtc atcaatgccg agcacgatgg
    31381 tcgccactgg cgacgaatac cagaacccat gcgactgtta gatgatgctg tggagcgctc
    31441 atcatgatga ccatcagcga tatcattgaa attattgtcg tttgcgcact gatatttttc
    31501 ccgctgggct atctggcgcg gcactctttg cgacgcattc gcgacacctt acgtttgttc
    31561 tttgctaaac ctcgttatgt taaaccggct gggacgttac gccgcacgga aaaagccagg
    31621 gcaaccaaaa aatgactcaa tttacgcaaa ataccgccat gccttcttcc ctctggcaat
    31681 actggcgcgg cctttccggc tggaacttct attttctggt taagttcggc ctgttgtggg
    31741 cgggatatct taacttccat ccgctcctca atttggtgtt tgccgcgttt ctgctgatgc
    31801 ccattccgcg ctacagcctg catcgcttgc gccactggat tgccctgccg atcggctttg
    31861 ctttgttctg gcatgacacc tggttgcctg gcccggaaag cataatgagc cagggttcgc
    31921 aggtggcggg gttcagtacc gattatttaa tcgaccttgt cacacgcttt attaactggc
    31981 agatgattgg ggccattttt gttttattag tggcctggtt attcctgtca caatggattc
    32041 gcattaccgt ttttgtggtt gccattctgc tgtggctgaa cgtacttacc ctggcgggac
    32101 caagtttctc cttgtggcca gccggtcaac cgacgaccac tgtaacaacg acgggtggta
    32161 acgcagcggc aaccgttgcg gcgacggggg gcgcaccggt agtgggtgat atgcccgcac
    32221 aaactgcacc gccaacaacg gcgaacctta acgcctggct gaataatttc tataacgcgg
    32281 aggcgaaacg taaatcgacc ttcccgtctt cgctgcccgc tgatgctcag ccatttgaac
    32341 tactggtgat taatatttgt tcgctttcct ggtcggatat tgaagccgcc gggttgatgt
    32401 cgcatccact gtggtcgcat ttcgatattg agttcaagaa ctttaactcc gccacctcct
    32461 acagtggccc ggcagcgatc cgtttactgc gcgccagctg cgggcagact tcgcacacta
    32521 atctgtatca accggcaaat aacgactgct atctgtttga taacctttcg aaactgggct
    32581 ttacccagca cctgatgatg ggacataacg gccagttcgg cggctttttg aaagaagttc
    32641 gcgaaaatgg cggcatgcag agcgaattga tggatcaaac aaatctgccg gttattttac
    32701 tgggctttga tggttcgccg gtttatgacg ataccgccgt gcttaaccgc tggttggacg
    32761 ttaccgaaaa agataaaaac agccgtagtg ccacgttcta caacacgctt ccactgcatg
    32821 acggcaacca ttatccgggc gtcagcaaaa cagcggatta caaagcgcgg gcgcagaaat
    32881 tctttgatga actggacgcc ttctttactg aactggagaa atcgggtcgt aaagtgatgg
    32941 tggtcgtggt gccggaacac ggcggtgcgc tgaagggcga cagaatgcag gtatctggcc
    33001 tacgtgatat ccctagcccg tctatcaccg acgtccccgt tggggtgaaa ttcttcggca
    33061 tgaaggcacc acatcagggg gcaccgattg tcatcgacca accgagcagc ttcctggcta
    33121 tctccgatct ggtggttcgc gttcttgatg gcaagatttt caccgaaaac aatgttgact
    33181 ggaaaaaact caccagtggg ttgccacaaa cagcaccggt ctccgagaac tcaaatgcag
    33241 tagttattca ataccaggat aaaccgtacg ttcgcctgaa cggcggcgac tgggtgcctt
    33301 acccgcagta agcacgaaaa aggccgcaga gtttcacccc tgcggcctgg tccgggcgca
    33361 aattgccatt acggcagcct gacgcccgca tgacacgtta cttccgtttg ttcagccagt
    33421 tcacgatcat actggcaaga atgccagcaa tgaccggagc cgctaaatca tgccagaagg
    33481 ccatgcccag ctctgcgagc gtcatatagc cgcctgtgtt gtaatgacaa cgtttcgcgg
    33541 ctattcttga gtggtctaga gtcaagatta gcccccgtgg tgttgtcagg tgcatacctg
    33601 caacgtgcgg gggttttctc tctccagcaa ccaatgccac cagggataaa gcccccgcaa
    33661 cattgcgcct caccggaacc tttccggctt gccactgatt ctacgactgt ttttctgttc
    33721 cggaatccgt cacgtcttca ctctgtgctg cgcattccgc agaatattat ccgcttgcaa
    33781 tcgttgcatt tttcttacga aaaaggccgc agagtttccc ccctgcggcc tggtccgggc
//
LOCUS       DAZFFG010000038        33692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFFG010000038 DAZFFG010000000
VERSION     DAZFFG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.038"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..305)
                     /locus_tag="ACHJ7J_002198"
     CDS             complement(<1..305)
                     /locus_tag="ACHJ7J_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO5000432.1"
                     /translation="MNNTLLPSINIPCTLFETISLFNDYSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     "
     gene            492..638
                     /locus_tag="ACHJ7J_002199"
                     /pseudo
     CDS             492..638
                     /locus_tag="ACHJ7J_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(679..1059)
                     /gene="yigF"
                     /locus_tag="ACHJ7J_002200"
     CDS             complement(679..1059)
                     /gene="yigF"
                     /locus_tag="ACHJ7J_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFO5000433.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(1073..1453)
                     /gene="yigG"
                     /locus_tag="ACHJ7J_002201"
     CDS             complement(1073..1453)
                     /gene="yigG"
                     /locus_tag="ACHJ7J_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFO5000434.1"
                     /translation="MLRMFIPTSNGKISRRRYIFSFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKTYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(1548..2438)
                     /gene="rarD"
                     /locus_tag="ACHJ7J_002202"
     CDS             complement(1548..2438)
                     /gene="rarD"
                     /locus_tag="ACHJ7J_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO5000435.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(2490..2957)
                     /gene="yigI"
                     /locus_tag="ACHJ7J_002203"
     CDS             complement(2490..2957)
                     /gene="yigI"
                     /locus_tag="ACHJ7J_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO5000436.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3122..3991
                     /gene="pldA"
                     /locus_tag="ACHJ7J_002204"
     CDS             3122..3991
                     /gene="pldA"
                     /locus_tag="ACHJ7J_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO5000437.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4124..5953
                     /gene="recQ"
                     /locus_tag="ACHJ7J_002205"
     CDS             4124..5953
                     /gene="recQ"
                     /locus_tag="ACHJ7J_002205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO5000438.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMAGCRTGQIRLLYIAPERLMLDNFLEHLAHWKPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            6017..6637
                     /gene="rhtC"
                     /locus_tag="ACHJ7J_002206"
     CDS             6017..6637
                     /gene="rhtC"
                     /locus_tag="ACHJ7J_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO5000439.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6699..7319)
                     /gene="rhtB"
                     /locus_tag="ACHJ7J_002207"
     CDS             complement(6699..7319)
                     /gene="rhtB"
                     /locus_tag="ACHJ7J_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO5000440.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7430..8452
                     /gene="pldB"
                     /locus_tag="ACHJ7J_002208"
     CDS             7430..8452
                     /gene="pldB"
                     /locus_tag="ACHJ7J_002208"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO5000441.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8460..9260
                     /gene="yigL"
                     /locus_tag="ACHJ7J_002209"
     CDS             8460..9260
                     /gene="yigL"
                     /locus_tag="ACHJ7J_002209"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO5000442.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9336..10235
                     /gene="bioP"
                     /locus_tag="ACHJ7J_002210"
     CDS             9336..10235
                     /gene="bioP"
                     /locus_tag="ACHJ7J_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO5000443.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10123..11076)
                     /gene="metR"
                     /locus_tag="ACHJ7J_002211"
     CDS             complement(10123..11076)
                     /gene="metR"
                     /locus_tag="ACHJ7J_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO5000444.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSSMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11312..13573
                     /gene="metE"
                     /locus_tag="ACHJ7J_002212"
     CDS             11312..13573
                     /gene="metE"
                     /locus_tag="ACHJ7J_002212"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO5000445.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13613..14428)
                     /gene="ysgA"
                     /locus_tag="ACHJ7J_002213"
     CDS             complement(13613..14428)
                     /gene="ysgA"
                     /locus_tag="ACHJ7J_002213"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO5000446.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGSQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            14690..15451
                     /gene="udp"
                     /locus_tag="ACHJ7J_002214"
     CDS             14690..15451
                     /gene="udp"
                     /locus_tag="ACHJ7J_002214"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO5000447.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            15592..17019
                     /gene="rmuC"
                     /locus_tag="ACHJ7J_002215"
     CDS             15592..17019
                     /gene="rmuC"
                     /locus_tag="ACHJ7J_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO5000448.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17114..17869
                     /gene="ubiE"
                     /locus_tag="ACHJ7J_002216"
     CDS             17114..17869
                     /gene="ubiE"
                     /locus_tag="ACHJ7J_002216"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO5000449.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            17883..18488
                     /gene="ubiJ"
                     /locus_tag="ACHJ7J_002217"
     CDS             17883..18488
                     /gene="ubiJ"
                     /locus_tag="ACHJ7J_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO5000450.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18485..20125
                     /gene="ubiB"
                     /locus_tag="ACHJ7J_002218"
     CDS             18485..20125
                     /gene="ubiB"
                     /locus_tag="ACHJ7J_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO5000451.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20204..20473
                     /gene="tatA"
                     /locus_tag="ACHJ7J_002219"
     CDS             20204..20473
                     /gene="tatA"
                     /locus_tag="ACHJ7J_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO5000452.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20477..20992
                     /gene="tatB"
                     /locus_tag="ACHJ7J_002220"
     CDS             20477..20992
                     /gene="tatB"
                     /locus_tag="ACHJ7J_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO5000453.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            20995..21771
                     /gene="tatC"
                     /locus_tag="ACHJ7J_002221"
     CDS             20995..21771
                     /gene="tatC"
                     /locus_tag="ACHJ7J_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO5000454.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            21813..22595
                     /gene="tatD"
                     /locus_tag="ACHJ7J_002222"
     CDS             21813..22595
                     /gene="tatD"
                     /locus_tag="ACHJ7J_002222"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO5000455.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22592..23080)
                     /gene="rfaH"
                     /locus_tag="ACHJ7J_002223"
     CDS             complement(22592..23080)
                     /gene="rfaH"
                     /locus_tag="ACHJ7J_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO5000456.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPTTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23247..24740
                     /gene="ubiD"
                     /locus_tag="ACHJ7J_002224"
     CDS             23247..24740
                     /gene="ubiD"
                     /locus_tag="ACHJ7J_002224"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO5000457.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            24786..25487
                     /gene="fre"
                     /locus_tag="ACHJ7J_002225"
     CDS             24786..25487
                     /gene="fre"
                     /locus_tag="ACHJ7J_002225"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO5000458.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(25679..26842)
                     /gene="fadA"
                     /locus_tag="ACHJ7J_002226"
     CDS             complement(25679..26842)
                     /gene="fadA"
                     /locus_tag="ACHJ7J_002226"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO5000459.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(26852..29041)
                     /gene="fadB"
                     /locus_tag="ACHJ7J_002227"
     CDS             complement(26852..29041)
                     /gene="fadB"
                     /locus_tag="ACHJ7J_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO5000460.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARVLVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAR
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            29231..30562
                     /gene="pepQ"
                     /locus_tag="ACHJ7J_002228"
     CDS             29231..30562
                     /gene="pepQ"
                     /locus_tag="ACHJ7J_002228"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO5000461.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            30562..31176
                     /gene="yigZ"
                     /locus_tag="ACHJ7J_002229"
     CDS             30562..31176
                     /gene="yigZ"
                     /locus_tag="ACHJ7J_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO5000462.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31215..32666
                     /gene="trkH"
                     /locus_tag="ACHJ7J_002230"
     CDS             31215..32666
                     /gene="trkH"
                     /locus_tag="ACHJ7J_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO5000463.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            32678..33223
                     /gene="hemG"
                     /locus_tag="ACHJ7J_002231"
     CDS             32678..33223
                     /gene="hemG"
                     /locus_tag="ACHJ7J_002231"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO5000464.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            33601..>33692
                     /locus_tag="ACHJ7J_002232"
     rRNA            33601..>33692
                     /locus_tag="ACHJ7J_002232"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7992 a         8442 c         9078 g         8180 t
ORIGIN      
        1 gaaaacattt ttgctagttc tttcatttct gcaaataaaa tatcagtgca ttcacgttgt
       61 gatatttttg ttccggaact tgaggcacta aaagcaacat taatagaacc tgggccaggg
      121 aaatggtatt taataagtct gtaaggatca acttttgctg agatatcgct taggccgagt
      181 gagagaaaat cttgttctac catatcacca tattgcatat catcagcaga ataatcatta
      241 aataaactaa tagtttcgaa aagtgtacag ggaatattaa ttgatggaag taatgtgtta
      301 ttcatttaca ttttccttgg ttatatttaa gctcaatatc aatttgagaa caaatgctag
      361 ggtaaaaaaa tatttaacaa agaagtacac ttcacatttt ttcattacat ctctgaagag
      421 aacgtcgtta atgattggtc ttgctcattg ttaataacaa aatattgaat ttaccgatta
      481 ggaaactttt tcctacgccg attgcctcca gctatggcat gaactttgag tttatgccag
      541 aactgaagtg gagtttcggc tacccaggcg cgattatctt tatgatcctc gcgggcctgg
      601 caccgtatct gtactttaag cgcaagaact ggttgtaaaa agaaggcggt ggcttagcct
      661 ggctaagcca cctcttattc aaaggctcca ggtatttaac ccttttacct ctttctcata
      721 gaaccatttg ttcgtgttaa cagcaacata ggctgctacg gcaattccca gaatgttaac
      781 gccaattaat gcaccaacaa atagagatag aatgccaagt aatagaacta taattgcttt
      841 tttccataag cccagaacaa ataaatagat ccaggaacag aagaaagcaa tgaaattcat
      901 ttgaatagtt aatcgttgtc ttacttttaa tgctttaaat gctgctttat attctggtgt
      961 tgctccccag aatccaggaa agccatgttg atcataaaaa ttaaatcggt atttccattt
     1021 ttcacttaat gaaccatcgt tcatatattc cttactcata aatactccat aactattgtt
     1081 ttgatgaatc agtaggtgca agcattagca tactgaaagt ggaaaaataa caaatcaaaa
     1141 aaatcatcga accattgcct gaacaggcaa aatcttcggc tatcattgtg atgatagaga
     1201 tgatatatac tgctaatgta ccaaaaacat aagtttttat atagatgaaa ccactatcac
     1261 ggagtcgttg gcaattcatg ttgatgacga gataatggag tgcgatggta gagactataa
     1321 caagaaaacc tgcttctcca tcgttaaaaa agataataag aaaggcaaaa atgaagttta
     1381 ttaaaataaa tgaaaatata taacggcgtc tggaaatctt accgttagat gttgggataa
     1441 acatacgtaa cataaatttt acatccttgt gcgagtctcc tgtcagcatg gcaatatgcc
     1501 cactctcatg caagagtcgg catatttttt cagaatatat ttatttttta tttggacgtt
     1561 ctacgttgag tataaattgc atccatcaca aaaattgcca gcgccaccca aataaaggcg
     1621 aaagtcacca ttttatcggc acccggtttt tcaccataaa acgtcacagc cagcaggaac
     1681 atcagcgtcg ggccaatgta ctggaaaaag cctaacgttg agagacgcaa gcgcgtggca
     1741 gcggcggtaa aacacaacag cggtacggta gtgacaatac cggcggcgat cagcagtaaa
     1801 ttcagcgaca tcgggttttg ccccatatgg ctggttgagc tgtcggcaat agcaaacagg
     1861 taaattgccg ccacgggcag cagccacata gtttcgatta acatgccggt ttgcgcttca
     1921 acggcaatct tcttgcgtac cagaccgtag aaggcaaaac taaatgccag tcccagcgcg
     1981 ataataggta gcgaaccaaa agtccacagc tggactaaca cgccacatat cgccagaatc
     2041 accgccagcc attgcatccg gcggaatcgc tcgccgagga aaatcatccc caacacaatg
     2101 ttcaccagtg ggttaataaa gtaaccaagg ctcgcttcca gcatatggtg gttgtttacc
     2161 gcccagataa acagtaacca gttgccgcca atcagcacgg cagagactgc cagcataaaa
     2221 attttctgtg gcgtctgaat cagcgttttt aaataggacc actggcggca aatgctcatc
     2281 agcaccacca taaagaaaaa cgaccagatc acgcgatgcg tcaggatttc atcggcgggc
     2341 acgtagtaaa tcaacttgaa gtacgctggc gcaatacccc aaataaaata agcggcaaga
     2401 gcgagtaata cgccctgccg cgtttgtttt gcatccatcg ggaatactca tttttaattg
     2461 gtaacagcag tttacctgct tttatgtctt caacctacca tataggtggc ggtggcactg
     2521 gcaatataaa gctgttcttc attgtgtaat tcaacgcggg cgacggcgac tttattgcct
     2581 gcacgcaaca ggctactggt agcagtaaaa cgctcgcctc tgcctgggcg cagataatcg
     2641 acgcgaagat caatggtccc catccgcgat agccgctggc gtagttcatc ttcactgatg
     2701 gtttcgtggc gggttaaggt acttcctacg cacaccagac cggcggcgac atccagcgcc
     2761 gacgcaatga ccccgccgtg caaaatgctt tgcgcccagt tgcccaccat cattggctga
     2821 tttttaaagg ccagctgtgc gaactctttt tcgtaacgct ccagttccat ccccaatgcg
     2881 cggttaaatg gcatgtgata aacaaacatc tcacccacta atttcagggc ttgttcagcg
     2941 gtcagtacgg cagacatatc atccttacac ctcattggtt aatgaaatgt tgattttatg
     3001 cttctttgtt gttggtttct actttaggaa gggataacta acggctatgg agttaagtat
     3061 gtaaaatagc ccgcagaaaa atattcaact tatcaataat tcgttacgga gaacacgacc
     3121 gatgcggact ctgcagggct ggttgttgcc ggtgtttatg ttgcctatgg cagtatatgc
     3181 acaagaggca acggtgaaag aggtgcatga cgcgccagcg gtgcgtggca gtattatcgc
     3241 caatatgctg caggagcatg acaatccgtt cacgctctat ccttatgaca ccaactacct
     3301 catctacaca caaaccagcg atctgaataa agaagcgatt gccagttacg actgggcgga
     3361 aaatgcgcgt aaggatgaag taaagtttca gttgagcctg gcatttccgc tgtggcgtgg
     3421 gattttaggc ccgaactcgg tgttgggtgc gtcttatacg caaaaatcct ggtggcaact
     3481 gtccaatagc gaagagtctt caccgtttcg tgaaaccaac tacgaaccgc aattgttcct
     3541 cggttttgcc accgattacc gttttgcagg ctggacgttg cgcgatgtag agatggggta
     3601 taaccacgac tctaacgggc gttcagatcc gacttcccgc agctggaacc gcctttatac
     3661 tcgcctgatg gcagaaaacg gtaactggct ggtagaagtg aagccgtggt atgtggtggg
     3721 taatactgac gataacccgg atatcaccaa atatatgggt tactaccagc ttaaaatcgg
     3781 ctatcacctc ggtgatgcgg tgctcagtgc gaaaggacag tacaactgga acaccggcta
     3841 cggcggcgcg gagttaggct taagttaccc gatcaccaaa catgtgcgcc tttatactca
     3901 ggtttacagc ggctatggcg aatcgctcat cgactataac ttcaaccaga cccgtgtcgg
     3961 tgtgggggtt atgctaaacg atttgttttg atgaacggtt gagtggttgg caaatctgga
     4021 atccagcatc caggattacc ctctcagaga ctaaaagcat tgcagtttct cgcgcaggcg
     4081 ctgaaaatag cgcctgtttt tatttcaggc aatcggggtg aatgtggcgc aggcggaagt
     4141 gttgaatctg gagtccggag ctaaacaggt tttacaagaa acctttggct accaacagtt
     4201 tcgccccggc caggaagaaa ttatcgacac tgtgctttcc ggtcgcgatt gcctggtcgt
     4261 catgcccacc ggtggcggaa aatccctttg ctatcaaatt cctgccttat tgctaaacgg
     4321 ccttaccgtg gttgtttcac cgctgatttc gttgatgaaa gatcaggtgg atcaactgca
     4381 agccaacggc gtggcggcgg cgtgcctgaa ctcaacgcaa acccgcgagc agcagcttga
     4441 ggtcatggcg ggatgccgta cagggcaaat tcgcctgctc tatatcgccc cggaacgcct
     4501 gatgctggat aacttccttg agcatctggc gcactggaag ccggtgttat tagccgttga
     4561 tgaagcccac tgtatttccc aatggggcca cgatttccgc ccggaatatg ccgcgctcgg
     4621 tcagttgcgc cagcggttcc cgacgctgcc gtttatggcg ctgaccgcca cagccgacga
     4681 caccacgcgc caggatatcg tgcgcctgct ggggctgaac gatccgctga ttcaaatcag
     4741 cagttttgac cgtccgaata ttcgctacat gctgatggag aaattcaaac cgctcgatca
     4801 gttgatgcgc tacgtgcagg aacagcgcgg taagtccggc attatctact gcaacagccg
     4861 ggcgaaagta gaagacaccg ctgcgcgcct gcaaagcaag ggtattagcg cggcggccta
     4921 tcatgccggg ctggaaaata atgtccgcgc cgacgtgcag gagaaattcc agcgcgatga
     4981 cttgcaaatt gtggtggcga cggtggcgtt cggcatgggc atcaataaac caaacgttcg
     5041 cttcgtggtc cactttgata ttccgcgcaa tatcgaatcc tattatcagg aaaccggtcg
     5101 cgccgggcgt gatggcctgc ctgcggaagc gatgttgttt tacgatccag ctgatatggc
     5161 gtggctgcgc cgttgtctgg aagagaagcc gcaggggcag ttgcaggata tcgagcgcca
     5221 caaactcaat gcgatgggcg cgtttgccga agcgcaaact tgccgtcgtc tggtattgct
     5281 gaactatttt ggcgaagggc gtcaggagcc gtgcgggaac tgcgatatct gcctcgatcc
     5341 gccgaaacag tacgacggtt caaccgatgc tcagattgcc ctttccacca ttggtcgtgt
     5401 gaatcagcgg tttgggatgg gttatgtggt ggaagtgatt cgtggtgcta ataaccagcg
     5461 tatccgcgac tatggtcatg acaaactgaa agtctatggc atgggccgtg ataaaagcca
     5521 tgaacattgg gtgagcgtga tccgccagct gattcacctc ggcctggtga cgcaaaatat
     5581 tgcccagcat tctgccctac aactgacaga ggccgcgcgc ccggtgctgc gcggcgaatc
     5641 ctctttgcaa cttgccgtgc cgcgtatcgt ggcgctcaaa ccgaaagcga tgcagaaatc
     5701 gttcggcggc aactatgatc gcaaactgtt cgccaaatta cgcaaactgc gtaaatcgat
     5761 tgccgatgaa agcaatgtcc cgccgtacgt ggtgtttaac gacgcaacct tgattgagat
     5821 ggctgaacag atgccgatca ccgccagcga aatgctcagc gttaacggcg ttgggatgcg
     5881 caagctggaa cgctttggta aaccgtttat ggcgctgatt cgtgcgcatg tcgatggcga
     5941 cgacgaagag tagtcagcag cataaaaaag tgccaggatg aagactccgt aaacgtttcc
     6001 cccgcgagtc aaatgtatgt tgatgttatt tctcaccgtc gccatggtgc acattgtggc
     6061 gcttatgagt cccggtcccg atttcttttt tgtctctcag accgctgtca gtcgttcccg
     6121 taaagaagcg atgatgggcg tgctcggtat tacctgcggc gttatggttt gggcagggat
     6181 tgcgttgctt ggcctgcatt tgattatcga aaaaatggcc tggctgcata cgctgattat
     6241 ggttggcggt ggcctgtatc tctgctggat gggttaccag atgctgcgcg gcgcgctgaa
     6301 aaaagaggcg gtttctgcac ctgcgccaca ggtcgagctg gcgaaaagtg ggcgcagttt
     6361 cctgaaaggt ttactgacca atctcgctaa tccgaaagcg attatctact ttggctcggt
     6421 gttctcattg tttgtcggtg ataacgttgg cactaccgcg cgctggggca tttttgcgct
     6481 gatcattgtc gaaacactgg cgtggtttac cgtcgttgcc agcctgtttg ccctgccgca
     6541 aatgcgccgt ggttatcaac gtctggcgaa gtggattgat ggttttgccg gggcgttatt
     6601 tgccggattt ggcattcatt tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa
     6661 gtcggataag gcgtttacgc cgcatccgac attatttttc acgcatgcct cgccgatgct
     6721 aacagcgctc ccaccagcat aaacaacgag ccgaaaatct tattcagcgc cttcatctgc
     6781 tttggtcctt taatccatag agcaatccgt tgagcaaggg tggcgtaacc gatcatcaca
     6841 ataatatcga ccacaatagt ggtgacgccg agcacgatat actgcatcag ttgcggctgt
     6901 tgcggcatga tgaattgcgg aaatagcgcc gccagaaaca caatactttt gggattggtg
     6961 agattcacaa aaactgcgcg ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat
     7021 ttaagatcaa ttgcgccagc ggcgcgccat tgctgtattc ccagccaaat caagtaagcc
     7081 gcgcctgccc acttcaacac ttcaaacgca atcactgagc gggaaaatag cgtccccaac
     7141 cccacgccaa ccagcacaat atgaatcgcc agtccggtct gaagcccggc aatagacgcc
     7201 accgcgccgc gataaccgtg gttgagcgag gtggtcatag tgttgattgc accagagcct
     7261 ggcgacaggc ttaaaatgat tgatgtcagt aggtaggcaa accaccattc taaggtcatg
     7321 atgaactccc ggtgtgtcta tttttgtgcc acaatacgct actgtcgcag cgttgtgtca
     7381 ggcacgctaa aaaaaacgat tttacgtggt ttaagaggca gattacccga tgtttcagca
     7441 gcaaaaagac tgggaaacaa gagaaaacgc gtttgctgct tttaccatgg gaccgctgac
     7501 tgatttctgg cgtcagcgtg atgaagcaga gtttactggt gtggatgaca ttccggtgcg
     7561 ctttgtccgt tttcgcgcac agcaccatga ccgggtggta gtcatctgcc cggggcgtat
     7621 tgagagctac gtaaaatatg cggaactggc ctacgatttg ttccatttgg ggtttgatgt
     7681 cttaatcatc gaccatcgcg ggcagggacg ttccggtcgc ctgttagccg atccgcatct
     7741 cgggcatgtt aatcgcttta atgattatgt tgatgatctg gcggcattct ggcaacagga
     7801 ggtccagccc ggtccgtggc gtaaacgcta tatactggca cattcgatgg gcggtgcgat
     7861 ctccacatta tttctgcaac gccatccagg tgtatgtgac gccattgcgc taactgcgcc
     7921 aatgtttggg atcgtgattc gtatgccgtc atttatggca cggcagatcc tcaactgggc
     7981 cgaagcgcat ccacgtttcc gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt
     8041 gccgtttgct atcaacgtac tgacccacag cagacagcga tatcgacgta acttacgctt
     8101 ctatgctgat gacccaacga ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag
     8161 tattctggct ggcgaacagg tgttagccgg tgcgggtgat gacgccacgc caacgcttct
     8221 cttgcaggct gaagaggaac gcgtggtgga taaccgcatg catgaccgtt tttgtgaact
     8281 ccgcaccgcc gcgggccatc ctgtcgaagg aggacggccg ttggtaatta aaggtgctta
     8341 ccatgagatc ctttttgaaa aggacgcaat gcgctcagtc gcgctccacg ccatcgttga
     8401 ttttttcaac aggcataact cacccagcgg aaaccgctct acagaggttt aaatttctta
     8461 tgtaccaggt tgttgcgtct gatttagatg gcacgttact ttctcccgac catacgttat
     8521 ccccttacgc caaagaaacc ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg
     8581 cgaccggtcg tcaccacgtt gatgtggggc aaattcgcga taatctggag attaagtctt
     8641 acatgattac ctccaatggt gcgcgcgttc acgatctgga tggtaatctg atttttgctc
     8701 ataacctgga tcgcgacatt gccagcgatc tgtttggcgt agtcaacgac aatccggaca
     8761 tcattactaa cgtttatcgc gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga
     8821 tgcgcttttt taaagaagcg gtgttcaaat atgcgctgta tgagcctgga ttactggagc
     8881 cggaaggcgt cagcaaagtg ttcttcacct gcgattccca tgaacaactg ctgccgctgg
     8941 agcaggcgat taacgctcgt tggggcgatc gcgtcaacgt cagtttctct accttaacct
     9001 gtctggaagt gatggcgggc ggcgtttcaa aaggccatgc gctggaagcg gtggcgaaga
     9061 aactgggcta cagcctgaag gattgtattg cgtttggtga cgggatgaac gatgccgaaa
     9121 tgctgtcgat ggcggggaaa ggctgcatta tgggcagtgc gcaccagcgt ctgaaagacc
     9181 ttcatcccga gctggaagtg attggtacta atgccgacga cgcggtgccg cattatctgc
     9241 gtaaactcta tttatcgtaa tcgttcttta tttggtcagt tgtcaacctg atacttcgct
     9301 acaatggata ctcgttaatc aaagagtttt ccattgtggc gctacttatc atcaccacga
     9361 ttctgtgggc cttctccttt agcttttatg gcgagtacct tgcggggcac gtcgatagct
     9421 attttgcggt gctggtgcgc gttggcctgg cggcactcgt ttttctgccg tttctgcgta
     9481 cccgtggcaa tagcctgaaa acagtcggcc tgtatatgct ggtgggcgcg atgcagctcg
     9541 gcgtgatgta tatgctgagt ttccgcgctt atctctacct gacggtttcc gaactgctgc
     9601 tgttcaccgt actgacgccg ctctacatca cgctgattta tgacatcatg agtaagcgcc
     9661 gtctgcgctg gggatatgct tttagtgcct tgttggcggt gattggtgcc gggattattc
     9721 gctatgatca ggtcaccgac catttctgga ctggcttgtt gctggtgcaa ctctccaata
     9781 tcacttttgc catcggcatg gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc
     9841 agcataacgc ctttgcgtgg ttctatcttg gcgcgttcct ggtggcggta attgcatggt
     9901 tcttgctggg aaatgcgcag aaaatgccgc aaaccacgtt gcaatggggc attctggtgt
     9961 ttcttggcgt ggtggcttcc gggattggct actttatgtg gaactacggc gcaacccaag
    10021 tagacgccgg aacgctgggc attatgaata atatgcacgt tccggcaggg ctgctggtta
    10081 acctggctat ctggcaccaa cagccgcact ggccaacgtt tattacaggc gcgctggtga
    10141 tcctggcctc actgtgggtg catcgtaagt gggtcgctcc gcgctcttca caaacggcag
    10201 atgatcgcag gcgtgattgc gcgctgagcg aataaacgct tccgtaactg gctgacgctg
    10261 ctcgccatcg cgcacggcgg cgtacagtcg gctccacaag ccttcgccca gggttttggt
    10321 caccaccaga ccctggcgct caaaactctc tactacccaa tgcggcaggg cggcaatacc
    10381 catccgcgcg gcaaccatct gaatcaacaa taaggtgtta tcgacgcttt tcagtgacgg
    10441 gctgacgcct gccggctgaa gaaaatgccg ccagacatcc agtcggctac gctgcaccgg
    10501 ataaatcaac agcgtctcgc tggcgagatc ttccggtgta atacgcgttt ttgccgccaa
    10561 tggatggtca ggtgctaaca ccaggcgtac ttcatagtcg aacatcgacg aataatgcag
    10621 gccactgcgc ggcagaatat cggaggtcat caccagatcc agctcgccct gttgcaacgc
    10681 aggctgcggg tcaaacgtca cgcccgattt aaaatccatc tctacctgcg gccagttctt
    10741 atggaaattt tctaacgcgg gcgtcagcca ctgaatacag ctatggcact caatggcaat
    10801 gcgcagacgc gtctgctgcg gttcattgca ggcttgcagt gcctggctaa tttgcggcag
    10861 cacctggttt gccagttgca acaggatttc tccctgcggt gtaaagcgaa gcggctggct
    10921 tttacgcaca aatagccgga agccaaggcg ttgttccaga tcgctaaact ggtgagacag
    10981 ggcggattgc gtctgatgca acgtcgccgc agcggctgcg agagagccgc agttccgcaa
    11041 cgcttgtagc gttttcaggt gttttacttc gatcatgaaa gtccttcact tcggcatgaa
    11101 taatttgcgc ttgaggaata tacagtaacc gccaattatg gatgtgtaaa catctggacg
    11161 gctaaaatcc ttcgtctttt aaatttatgg tgcgttggct gcgtttctcc accccggtca
    11221 cttacttcag taagctcccg gggatgaata aacttgccgc cttccctaaa ttcaaaatcc
    11281 ataggattta catataatta gaggaagaaa aatgacaata ttgaatcaca ccctcggttt
    11341 ccctcgcgtt ggcctgcgtc gcgagctgaa aaaagcgcaa gagagttatt gggcggggaa
    11401 ctccacgcgt gaagaactgc tggcagtagg gcgtgaattg cgtgctcgtc actgggatca
    11461 acaaaagcaa gcgggtatcg acctgctgcc ggtgggcgat tttgcctggt acgatcatgt
    11521 actgaccacc agtctgctgc tgggtaacgt tccggcgcgt catcagaaca aagatggttc
    11581 ggtagatatc gacaccctgt tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc
    11641 ggcggcagcg gaaatgacca aatggtttaa caccaactat cactacatgg tgccggagtt
    11701 cgttaaaggc caacagttca aactgacctg gacgcagctg ctggacgaag tggacgaggc
    11761 gctggcgctg ggccacaagg tgaaacctgt gctgctgggg ccgattacct ggctgtggct
    11821 ggggaaagtg aaaggtgaac agtttgaccg ccttagcctg ctgaacgaca ttctgccggt
    11881 ttatcagcaa gtgctggcag aactggcgaa acgcggcatc gagtgggtac agattgatga
    11941 acccgcgctg gtactggaac taccacaggc gtggctggac gcatacaaac ccgcttacga
    12001 cgcgctccag ggacaggtga aactgctgct gaccacctat tttgaaggcg taacgccaaa
    12061 tctcgacacg attactgcgc tgcctgttca ggggctgcat gtggaccttg tacatggtaa
    12121 agatgacgtt gttgaactgc acaagcgcct gccttctgac tggctgctgt ctgcgggtct
    12181 gatcaatggt cgtaacgtct ggcgcgccga tcttactgag aaatatgcgc aaattaagga
    12241 cattgtcggc aaacgtgatt tgtgggtggc atcatcctgc tcgttgctgc acagccccat
    12301 cgacctgagc gtggaaacgc gtcttgatgc agaagtgaaa agctggtttg ccttcgccct
    12361 acaaaaatgc catgaactgg cactgctgcg cgatgcgctg aacagtggtg acacggcagc
    12421 tctggcagag tggagcgccc caattcaggc gcgtcgtcac tctactcgtg tacataaccc
    12481 ggcagtagaa aagcgtctgg cggcgatcac cgcccaggac agccagcgtg cgaatgtcta
    12541 tgaagtgcgt gccgaagccc agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac
    12601 gattggttcc ttcccgcaaa ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg
    12661 caatctcgac gccaataact accgcacggg cattgcggaa catatcaggc aggccattgt
    12721 tgaacaggaa cgtttgggac tggatgtgct ggtacatggc gaggccgagc gtaatgacat
    12781 ggtggaatat tttggcgagc acctcgacgg gtttgtcttt acgcaaaacg gttgggtaca
    12841 gagctatggt tcccgctgcg tgaagccacc gattgtcatt ggtgatatta gccgcccggc
    12901 accgattacc gtggagtggg cgaagtatgc gcaatctctg actgataaac cggtgaaagg
    12961 gatgctgact ggcccggtga ccattctctg ctggtcgttc ccgcgtgaag atgtcagccg
    13021 tgaaaccatc gccaaacaaa ttgcgctggc gctgcgtgat gaagtggcgg acctggaagc
    13081 cgcaggaatt ggcatcatcc agattgatga accggcgctg cgcgaaggtt taccgctgcg
    13141 tcgtagcgac tgggatgcat atctccagtg gggcgtggag gccttccgta tcaacgccgc
    13201 cgtggcgaaa gatgacacac aaatccacac tcacatgtgt tattgcgagt tcaacgacat
    13261 catggattcg attgcggcac tggatgcaga cgtcatcacc atcgaaacct cgcgttccga
    13321 catggagttg ctggagtcgt ttgaagagtt tgattatccg aatgaaatcg gtccaggcgt
    13381 ctatgacatt cactcgccaa acgtaccgag cgtagaatgg attgaagcct tgctgaagaa
    13441 agcagcacaa cgcattccgg cagagcgcct gtgggttaac ccggactgtg gtctgaaaac
    13501 gcgcggctgg ccagaaaccc gcgccgcgct ggcgaacatg gtacaggcgg cgcagaattt
    13561 gcgtcggggg taaaatccaa accgggtggt aataccaccc ggtcttttct cattacagcg
    13621 acttcttccc accatactgc ttaaaccatt ctagcatacg ctgccagcca tctttcgcag
    13681 attcggcatg atagctcggg cgataatcag cgttgaatgc atgcccggca tccgggtaca
    13741 cgataatctc tgctttcgca ttagcagccc gcagcgcctg gcgcatggtt tcaacgctct
    13801 cctgcggaat gctgttatcc tgactgccat ataagccgag aaccggcgcg ttaagatcgg
    13861 ttgcgatatc aacaggttgt ttcggtgaat tcagcgactt gtcgcccgtc agtttgccgt
    13921 accacgccac tgcggctttt agctgtggat tatgtgcggc atacagccag gtgatacgtc
    13981 caccccagca gaatccggtg atcattaaac gatgaacatc gccgccgttg cgggaagccc
    14041 aactggcgac atgatcgaga tcggccagca cctgcgagtc aggcactttt gctaccagac
    14101 cgctaagcaa cgtggggata tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt
    14161 caggtgcgat agccagatac ccctccagcg ccagacggcg acaaatgtcg cggatatgtt
    14221 catgcacgcc aaaaatttcc tgcactacaa tgaccactgg cagtgggcca tcgctttgct
    14281 ttggtctggc atggtaagca ggcatgttat ccccttgtga agggatagag gtgaagcccg
    14341 ccacaatcgc gtcgtccggg gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg
    14401 caaatccaga ttgttgtgtt gttgccatgg tattctccgt acccttataa aaatgttgcg
    14461 caatgttaac tatagtcagc atgcaacaaa tcacattgcc tgaatcggct catcttttat
    14521 gcagtcctgc agaatgaaga gtgatttatg tgatttgcat cacttttggt gggtaaattt
    14581 atgcaacgca tttgcgtcat ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa
    14641 agtgtctttt tgcttcttct gactaaaccg attcacagag gagttgtata tgtccaagtc
    14701 tgatgttttt catctcggcc tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt
    14761 ccctggcgac ccggatcgtg tggaaaagat cgccgcgctg atggataagc cggttaagct
    14821 ggcatctcac cgcgaattca ctacctggcg tgcagagctg gatggtaaac ctgttatcgt
    14881 ctgctctacc ggtatcggcg gcccgtctac ctctattgct gttgaagagc tggcacagct
    14941 gggcattcgc accttcctgc gtatcggtac aacgggcgct attcagccgc atattaatgt
    15001 gggtgatgtc ctggttacca cggcgtctgt tcgtctggac ggcgcgagcc tgcacttcgc
    15061 accgctggaa ttcccggctg tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc
    15121 gaaatccatt ggcgcgacaa ctcacgttgg cgtgacagct tcttctgata ccttctatcc
    15181 aggtcaggaa cgttacgata cttactctgg tcgcgtagtt cgtcacttta aaggttctat
    15241 ggaagagtgg caggcgatgg gcgtaatgaa ctatgaaatg gaatctgcaa ccctgctgac
    15301 catgtgtgca agtcagggcc tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac
    15361 ccagcaagag atcccgaatg ctgagacgat gaaacaaact gaaagccaag cggtgaaaat
    15421 cgtggtggaa gcggcgcgtc gtctgctgta attctcttct cctgtctgaa ggccgacgcg
    15481 ttcggccttt tgtatttttg cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt
    15541 gtacagcaca attctatttt gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc
    15601 tcaatcatgg tttacgcagt tattgcgttg gtgggtgtgg caattggctg gctgtttgcc
    15661 agttatcaac atgcgcagca aaaagccgag caattagctg aacgtgaaga gatggtcgcg
    15721 gagttaagcg cggcaaaaca acaaattacc caaagcgagc actggcgtgc agagtgcgag
    15781 ttactcaata acgaagtgcg cagcctgcaa agtattaaca cctctctgga ggccgatctg
    15841 cgtgaagtaa ccacgcggat ggaagccgca cagcaacatg ctgacgataa aattcgccag
    15901 atgattaaca gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa ccgtattttt
    15961 gagcacagca atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg
    16021 ccgctacgtg aacaactgga cggtttccgc cgtcaggttc aggacagctt cggtaaagaa
    16081 gcacaagaac gccataccct gacccacgaa attcgcaatc tccagcaact caacgcgcaa
    16141 atggcccagg aagcgatcaa cctgacgcgc gcgctgaaag gcgacaataa aacccagggc
    16201 aactggggcg aggtagtatt gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat
    16261 gaatatgaaa cccaggtcag catcgaaaat gacgcccgct cgcggatgca gccggatgtc
    16321 atcgtgcggc tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc
    16381 tatgaacgct attttaacgc cgaagacgac tacacccgcg aaagcgcgct acaggaacat
    16441 atcgcgtcgg tgcgtaacca tatccgtttg ctgggacgca aagattatca acagctgccg
    16501 gggctgcgaa ctctggatta cgtgctgatg tttattcccg ttgaacccgc ttttttactg
    16561 gcgcttgacc gccagccgga gctgatcacc gaagcgttga aaaacaatat catgctggtt
    16621 agcccgacta cgctgctggt ggcgctgcgc actatcgcca acctgtggcg ttatgagcat
    16681 caaagccgca acgcccagca aatcgccgat cgtgccagca agctgtacga caagatgcgt
    16741 ttgttcatcg atgacatgtc cgcgattggt caaagtctcg acaaagcgca ggataactat
    16801 cggcaggcaa tgaaaaaact ctcttcaggg cgcggaaatg tgctggcgca ggcagaagcg
    16861 tttcgcggtt taggagtaga aattaaacgc gagattaatc cggatttggc tgaacaggcg
    16921 gtgagccagg atgaagagta tcgacttcgg tcggttccgg agcagccgaa tgatgaagct
    16981 tatcaacgcg atgatgaata taatcagcag tcgcgctagc ccattgggag tagttaagcc
    17041 gggtagaaat ctagggcatc gacgcccaat ctgttacact tctggaacaa ttttttgatg
    17101 agcaggcatt gagatggtgg ataagtcaca agaaacgacg cactttggtt ttcagaccgt
    17161 cgcgaaggaa caaaaagcgg atatggtcgc ccacgttttc cattccgtgg catcaaaata
    17221 cgatgtcatg aatgatttga tgtcatttgg tattcatcgt ttgtggaagc gattcacgat
    17281 tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg gcaccggcga
    17341 cctgacagcg aaattctccc gcctggtcgg agaaactggc aaagtggtcc ttgctgatat
    17401 caatgaatcc atgctcaaaa tgggccgcga gaagctgcgt aatatcggtg tgattggcaa
    17461 cgttgagtat gttcaggcga acgctgaggc gctgccgttc ccggataaca cctttgattg
    17521 catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat
    17581 gtatcgcgtg ctgaaacccg gcggccgcct gctggtgctt gagttctcga agccaattat
    17641 cgagccgctg agcaaagcct atgatgcata ctccttccat gtgctgccgc gtattggctc
    17701 actggtcgcg aacgacgccg acagctaccg ttatctggca gaatccatcc gtatgcatcc
    17761 cgatcaggat accctgaaag ccatgatgca ggatgccgga ttcgaaagtg tcgactacta
    17821 caatctgacg gcaggggttg tggcgctgca tcgtggttat aagttctgac aggagaccgg
    17881 aaatgccttt taaaccttta gtgacggcag gaattgaaag tctgctcaac accttcctgt
    17941 atcgctcacc cgcgctgaaa acggcccgct cgcgtctgtt gggcaaagtg ctgcgcgtgg
    18001 aggtaaaagg cttttcgacg tcattgattc tggtgttcag cgaacgccag gttgatgtac
    18061 tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc ctacgccagt gtgttgccga
    18121 aacttcgcga tcgccagcag cttgccgccc tgattcgcag tggtgagctg gaagtgcagg
    18181 gcgatattca ggtggtgcaa aacttcgttg cgctggcaga tctggcagag ttcgaccctg
    18241 cggaactgct ggccccttat accggtgata tcgccgctga aggaatcagc aaagccatgc
    18301 gcggaggcgc aaagttcctg catcacggca ttaagcgcca gcaacgttat gtggcggaag
    18361 ccattactga agagtggcgt atggcacccg gtccgcttga agtggcctgg tttgcggaag
    18421 agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa aaactggagg
    18481 ctaaatgacg ccaggtgaag tacggcgcct atatttcatc attcgcactt ttttaagcta
    18541 cggacttgat gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc tatggcgata
    18601 ctcattattc tggatgccaa atcggcataa agacaaacct ttaggtgagc gactacgact
    18661 ggccctgcaa gaactggggc cggtatggat caagttcggg caaatgttat caacccgccg
    18721 cgatcttttt ccgccgcata ttgccgatca gctggcgtta ttgcaggaca aagttgctcc
    18781 gtttgatggc aagctggcga agcagcagat tgaagctgca atgggcggct tgccggtaga
    18841 agcgtggttt gacgattttg aaatcaagcc gctggcttct gcttctatcg cccaggttca
    18901 taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc gcccggatat
    18961 tttgccggtt attaaagcgg atctgaaact tatctaccgt ctggctcgct gggtgccgcg
    19021 tttgctgccg gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac
    19081 attgattgat gaactgaatt tgctgcggga atctgccaac gccattcagc ttcggcgcaa
    19141 ttttgaagac agcccgatgc tctacatccc ggaagtttac cctgactatt gtagtgaagg
    19201 gatgatggtg atggagcgca tttacggcat tccggtgtct gatgttgcgg cgctggagaa
    19261 aaacggcacc aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt
    19321 ctttcgcgac agctttttcc atgccgatat gcaccctggc aacatcttcg taagctatga
    19381 acacccggaa aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa
    19441 agaagataaa cgctatctgg cagaaaactt tatcgccttc tttaatcgcg actatcgcaa
    19501 agtggcagag ctacacgtcg attctggctg ggtgccacca gataccaacg ttgaagagtt
    19561 cgaatttgct attcgtacgg tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc
    19621 gtttggacat gtactgttaa atctgtttaa tacggcgcgt cgcttcaata tggaagtgca
    19681 gccgcaactg gtgttactcc agaaaaccct gctctacgtc gaaggggtag gacgccagct
    19741 ttatccgcaa ctcgatttat ggaaaacggc gaagcctttc ctggagtcgt ggattaaaga
    19801 tcaggtcggt attcctgcgc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga
    19861 aaaaatgcca gaactgcctg aactggttta cgacagtttg cgccagggca agtatttaca
    19921 gcacagtgtt gataagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatc
    19981 gcgttatttt ctcggaattg gcgctacgtt agtattaagt ggcacattct tgttggtcag
    20041 ccgacctgaa tgggggctga tgcccggctg gttaatggca ggtggtctga tcgcctggtt
    20101 tgtcggttgg cgcaaaacac gctgattttt tcatcgctca aggcgggccg tgtaacgtat
    20161 aatgcggctt tgtttaatca tcatctacca cagaggaaca tgtatgggtg gtatcagtat
    20221 ttggcagtta ttgattattg ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg
    20281 ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga
    20341 tgaaccaaag caggataaaa ccagtcagga tgctgatttt actgcgaaaa ctatcgccga
    20401 taagcaggcg gatacgaatc aggaacaggc taaaacagaa gacgcgaagc gccacgataa
    20461 agagcaggtg taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat
    20521 cggcctcgtc gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg
    20581 gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc aggagttaaa
    20641 actccaggag tttcaggaca gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac
    20701 gcccgaactg aaagcgtcga tggatgaact acgccaggcc gcggagtcga tgaagcgttc
    20761 ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg cacaccatcc ataacccggt
    20821 ggtgaaagat aatgaagctg cgcatgaggg cgtaacgcct gccgctgcac aaacgcaggc
    20881 cagttcgccg gaacagaagc cagaaaccac gccagagccg gtggtaaaac ctgctgcgga
    20941 cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt gataaaccgt aaacatgtct
    21001 gtagaagata ctcaaccgct tatcacgcat ctgattgagc tgcgtaagcg tctgctgaac
    21061 tgcattatcg cggtgatcgt gatattcctg tgtctggtct atttcgccaa tgacatctat
    21121 cacctggtat ccgcgccatt gatcaagcag ttgccgcaag gttcaacgat gatcgccacc
    21181 gacgtggcct cgccgttctt tacgccgatc aagctgacct ttatggtgtc gctgattctg
    21241 tcagcgccgg tgattctcta tcaggtgtgg gcatttatcg ccccagcgct gtataagcat
    21301 gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc tgctgtttta tatcggcatg
    21361 gcgttcgcct actttgtggt ctttccgctg gcatttggct tccttgccaa taccgcgccg
    21421 gaaggggtgc aggtatccac cgacatcgcc agctatttaa gcttcgttat ggcgctgttt
    21481 atggcgtttg gtgtctcctt tgaagtgccg gtggcaattg tgctgctgtg ctggatgggg
    21541 attacctcgc cagaagactt acgcaaaaaa cgcccgtatg tgctggttgg tgcattcgtt
    21601 gtcgggatgt tgctgacgcc gccggatgtc ttctcgcaaa cgctgttggc gatcccgatg
    21661 tactgtctgt ttgaaatcgg tgtcttcttc tcacgctttt acgttggtaa agggcgaaat
    21721 cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg
    21781 cccgtcaggg cggttgtcat atggagtaca ggatgtttga tatcggcgtt aatttgacca
    21841 gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta
    21901 atgggctact catcaccggc actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc
    21961 gtcagtattc atcctgttgg tcaacggcgg gcgtacatcc tcacgacagc agccagtggc
    22021 aagctgcgac tgaagaagcg attattgagc tggccgcgca gccagaagtg gtggcgattg
    22081 gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc ggaagagcag gaacgcgctt
    22141 ttgttgccca gctacgcatt gccgcagatt taaacatgcc ggtatttatg cactgtcgcg
    22201 atgcccacga gcggtttatg acattgctgg agccgtggct ggataaactg cctggtgcgg
    22261 ttcttcattg ctttaccggc acacgcgaag agatgcaggc gtgcgtggcg catggaattt
    22321 atatcggcat taccggttgg gtttgcgatg aacgacgcgg actggagctg cgggaacttt
    22381 tgccgttgat tccggcggaa aaattactga tcgaaactga tgcgccgtat ctgctccctc
    22441 gcgatctcac gccaaagcca tcatcccggc gcaacgagcc agcccatctg ccccatattt
    22501 tgcaacgtat tgcgcactgg cgtggagaag atgccgcatg gctggctgcc accacggatg
    22561 ctaatgtcaa aacactgttt gggattgcgt tttagagttt gcggaactcg gtgttcttca
    22621 cactgtgctt aatctcttta ttaataagat taagcaatag catggagcga gcctcaccat
    22681 cgggttcggt gaaaatggcc tgaaagcctt cgaacgcgcc ttcggtaata atcaccttat
    22741 cacccggata aggggttgtc ggatcgacaa tgtctttcgg tttatatacc gatagctgat
    22801 gaatcaccgc cgatgggact atcgctggcg acgcgccaaa gcgcacgaag tggctgacac
    22861 cgcgggtcgc gttgatagtc gtggtatgaa tcacttctgg atcaaattcc acaaacaggt
    22921 agttggggaa caatggctca ctgactgcag tacgtttgcc acgcacgatt ttttccaggg
    22981 tgatcatcgg tgccaggcaa ttcacagcct gtctttcgag gtgttcctgg gcacgttgaa
    23041 gttgcccgcg cttgcagtac agtaaatacc aggattgcat aatgactctt atccgtttaa
    23101 tcggggcgca aggatagcaa aagctttacg ctaagttaat tatattcccc ggtttgcgtt
    23161 ataccgtcag agttcacgct aatttaacaa atttacagca tcgcaaagat gaacgccgta
    23221 taatgggcgc agattaagag gctacaatgg acgccatgaa atataacgat ttacgcgact
    23281 tcttgacgct gcttgaacag cagggtgagc taaaacgtat cacgctcccg gtggatccgc
    23341 atctggaaat cactgaaatt gctgaccgca ctttgcgtgc cggtgggcct gcgctgttat
    23401 tcgaaaaccc taaaggctac tcaatgccgg tgctgtgcaa cctgttcggt acgccaaagc
    23461 gcgtggcgat gggcatgggg caggaagatg tttcggcgct gcgtgaagtt ggtaaattat
    23521 tggcgtttct gaaagagccg gagccgccaa aaggtttccg cgacctgttt gataaactgc
    23581 cgcagtttaa gcaagtattg aacatgccga caaagcggct gcgcggtgcg ccttgccaac
    23641 aaaaaatcgt ctctggcgat gacgtcgatc tcaatcgcat tcccattatg acctgctggc
    23701 cggaagatgc cgcgccgctg attacctggg ggctgacagt gacgcgcggc ccacataaag
    23761 agcggcagaa tctgggcatt tatcgccagc agctgattgg taaaaacaaa ctgattatgc
    23821 gctggctgtc gcatcgcggc ggcgcgctgg attatcagga gtggtgtgcg gcgcatccgg
    23881 gcgaacgttt cccggtttct gtggcgctgg gtgccgatcc cgccacgatt ctcggtgcag
    23941 tcactcccgt tccggatacg ctttcagagt atgcgtttgc cggattgcta cgtggcacca
    24001 agaccgaagt ggtgaagtgt atctccaatg atcttgaagt gcccgccagt gcggagattg
    24061 tgctggaagg gtatatcgaa caaggcgaaa ctgcgccgga agggccgtat ggcgaccaca
    24121 ccggttacta taatgaagtc gatagtttcc cggtatttac cgtgacgcat attacccagc
    24181 gtgaagatgc gatttaccat tccacctata ccgggcgtcc gccagatgag cccgcggtgc
    24241 tgggtgtcgc actgaacgaa gtgtttgtgc cgattctgca aaaacagttc ccggaaattg
    24301 tcgattttta cctgccgccg gaaggctgct cttatcgcct ggcggtagtg acaatcaaaa
    24361 aacagtacgc cggacacgcg aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt
    24421 ttatgtacac taaatttgtg atcgtttgcg atgatgacgt caacgcacgc gactggaacg
    24481 atgtgatttg ggcgattacc acccgtatgg acccggcgcg ggatactgtt ctggtagaaa
    24541 atacgcctat tgattatctg gattttgcct cgcctgtctc cgggctgggt tcaaaaatgg
    24601 ggctggatgc cacgaataaa tggccggggg aaacccagcg tgaatgggga cgtcccatca
    24661 aaaaagatcc agatgttgtc gcgcatattg acgccatctg ggatgaactg gctattttta
    24721 acaacggtaa aagcgcctga tgcgcgtttg ttttgcccta tttatcgatc cgacagagaa
    24781 agcgcatgac aaccttaagc tgtaaagtga cctcggtaga agctatcacg gataccgtat
    24841 atcgtgtccg catcgtgcca gacgcggcct tttcttttcg tgctggtcag tatttgatgg
    24901 tagtgatgga tgagcgcgac aaacgtccgt tctcaatggc ttcgacgccg gatgaaaaag
    24961 ggtttatcga gctgcatatt ggcgcttctg aaatcaacct ttacgcgaaa gcagtcatgg
    25021 accgcatcct caaagatcat caaatcgtgg tcgatattcc ccacggagaa gcgtggctgc
    25081 gcgatgatga agagcgtccg atgattttga ttgcgggcgg caccgggttc tcttatgccc
    25141 gctcgatttt gctgacagcg ttggcgcgta acccaaaccg tgatatcacc atttactggg
    25201 gcgggcgtga agagcagcat ctgtatgatc tctgcgagct tgaggcgctt tcgttgaagc
    25261 atcctggtct gcaagtggtg ccggtggttg aacaaccgga agcgggctgg cgtgggcgta
    25321 ctggcaccgt gttaacggcg gtattgcagg atcacggtac gctggcagag catgatatct
    25381 atattgccgg acgttttgag atggcgaaaa ttgcccgtga cctgttttgc agtgagcgta
    25441 atgcgcggga agatcgcctg tttggcgatg cgtttgcatt tatctgagat ataaaaaaac
    25501 ccgcccctga caggcgggaa gaacggcaac taaaacttta ttcagtggca tttagatcta
    25561 tgtcgcatcc ggcattcggc acacgatgcc tgatgcgacg ctggcgcgtc ttatcaggcc
    25621 tacattggtg ccggatcggt agaccggata aggcgttcac gccgcatccg gcaagtggtt
    25681 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    25741 caaactgaac gtctttgtgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    25801 aacaacccag cggatgaccc agcgcgatcg cgccaccgtt gaggttgatc ttctcgtcaa
    25861 tctgctcaat tagtcccaga tctttaatac atggcaggat ctgcgcggca aaggcttcat
    25921 tcatttcaaa cacgccgata tcactgacag aaagccccgc ttttttcagc gccagcttcg
    25981 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    26041 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    26101 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    26161 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    26221 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    26281 catttttaaa tgcggccgac tgcgtggcag cccaggcgcg ggcgtgtgac cgcgcggcaa
    26341 aggcatcctg catttcacgg ctgataccgt gcatacgcgc cagcatttct gccgttaagc
    26401 ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    26461 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    26521 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    26581 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    26641 tgttaaaacc ctgctccagc gtctgctgca cacaacccca gtaaatatcg tcgagggccg
    26701 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    26761 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    26821 cgacaatgac aacctgttcc attgtgactc cttaagccgt tttcaggtcg ccaaccggac
    26881 gggctggctc aaccggggga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    26941 ccggcacttc gtacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    27001 cgctgccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    27061 cgtagactag cgccatatcc gcttctgccg gagtggcgat aatgccttct tccagacagc
    27121 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    27181 cgcgcttcgg ctggctcact tcagccagca ggtcgtcaac gacggcgtct tcttctttct
    27241 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    27301 agcggttggc atcaaacagc gcgtcgatgg catcgcgata atctttctgc atccgctgcg
    27361 ggaagcctgc tgccatcacc gcctgagcgt gatgcgcggt gtcaatgccc acgacatcca
    27421 gcagatatgc cggacccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    27481 ggaaatcagc gccgtcgcgc agcaactggc tgaaaccggc gaaatacggg aacagcacgc
    27541 ggttgacgaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    27601 cccaggcgac gactttcgcg atggtttcgt cggagctttt ctcgccgcga attatttcta
    27661 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tctggacgtt
    27721 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    27781 ccgggcgtac tttctgctcg gtttctgcca gtacggcttt tttcactttt gggttttcga
    27841 caacagcttc taccacaaca tccacgcggt caaacccggc gtagtcgagc gttgggtgga
    27901 ttgtagagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    27961 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    28021 tgacaaccgg cacgcctttc ctcgcagact ggtaagcgat gccgccgccc ataatgcctg
    28081 cgcccagcac agcggcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    28141 cttttacata ttgatcgtta aggaatatgc cgactagtac gcgagcttcg ttggtatgcg
    28201 ccagcgggac aaaacttttg ttttccaggt ttaaggcttc ttcacgacca aaacgagccg
    28261 cagcttcaat ggtttttact gcggtgatgg gggccgggta atgttttcct gccgtttgtg
    28321 cgaccatccc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    28381 gttccagctt cggctgacgt ttcgctttcc agtcaaggtc gccgttaatg gcctggcgta
    28441 aaaccgccat cgccccttca accagttttt ctgctttgac tacgccatcc accagaccga
    28501 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc aatgatttcc agcgcactgt
    28561 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    28621 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    28681 cgcattcgca gccgccaccc agcgcgtagc cgttgacggc agcaatggtc ggcaccggca
    28741 gatcttccag gcgattaaac acgctattgg caaaatgcag ccactggctt aactgttctt
    28801 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    28861 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    28921 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    28981 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    29041 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgagtcga agtgtgtatt
    29101 ttgtgttaaa aatatgcaaa caaaagatta aaaaaatgcc gatctgatca cgctcggcag
    29161 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    29221 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    29281 acggactcgc gatgcgctgg cgcgcttcaa gctggatgcg ttacttattc actccggcga
    29341 gctgttcaat gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    29401 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    29461 gccgaaactg tggttttatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    29521 ctccttctgg actgaagatg tggaagtgat cgcgctgccg aaagccgatg gcattggtag
    29581 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg agcgtgcgct
    29641 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gttatcgact acctgcatta
    29701 ctatcgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    29761 ggtcaacggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    29821 caacattgcc tatctgaccg cgactggtca tcgtgatacc gacgtacctt acagcaacat
    29881 tgtggcgctt aacgaacacg ctgcggtgct gcattacacc aaactggatc atcaggcgcc
    29941 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgccgctga
    30001 cctgacccgt acctggtcgg caaaaagtga caacgattac gcacagctgg tgaaagacgt
    30061 aaatgatgaa caactggcgc tgatcgcgac catgaaagct ggcgttagct atgtggatta
    30121 ccacatccag ttccatcagc gcatcgccaa attgctgcgt aaacatcaaa tcatcaccga
    30181 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    30241 cggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    30301 tacgcacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    30361 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgctac tggcaccgtg
    30421 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    30481 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aataacgtgg aaaacatgac
    30541 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    30601 gttgaagaga tcaaaaaaag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    30661 gcggcgaaag cgtttgttga atcggtgcgg gcagaacatc ccgatgcccg ccaccattgt
    30721 gtggcgtggg tggcgggtgc gccggatgat tcacaacagt tgggtttctc tgacgacggg
    30781 gaaccggcgg gaacggcagg taaaccgatg ctcgcccaac tgatgggcag cggcgtcggg
    30841 gaaattaccg ccgtggtggt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    30901 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    30961 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    31021 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    31081 cgggtggcgc ttccggcagc gaaagtggct gaattttccg caaagctggc ggattttagc
    31141 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    31201 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    31261 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    31321 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    31381 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    31441 tctggaccgt gctgggtagt gtcggtgcgc tcccttttat tttctcggaa agcccgaacc
    31501 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    31561 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    31621 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    31681 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    31741 cgcgaattgc ggaaacggcg aaaaccctgt ggttgattta tgtcttgctg accgtcgcct
    31801 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    31861 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgatagcc
    31921 cgactattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    31981 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    32041 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac cctcgtactg tggtttcata
    32101 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtgtcgatgg
    32161 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    32221 tgcttttatg ttcagctttt atcggcggtt gtgccgggtc aacgggcggt ggcctgaaag
    32281 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    32341 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    32401 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    32461 tcgccacggg cgtggatgac ttttctgctt ttgcctcggt tgttgcgaca ttgaataacc
    32521 tggggccggg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    32581 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    32641 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    32701 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    32761 ggggatccag gcggatgtcg ccaatgtgca ccgcattgaa gaaccacagt gggaaaacta
    32821 cgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    32881 atttgtcaaa aagcatgcga cgcggctgaa ttcgatgccg agcgcctttt actccgtaaa
    32941 cctggtggcg cgtaaaccgg agaagcgcac tccacagacc aacagctacg cgcggaagtt
    33001 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    33061 cccacgttat cgctggtacg accgttttat gatcaagctg attatgaaga tgtcaggcgg
    33121 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    33181 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    33241 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcaga ccggaataac tccctataat
    33301 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcagg gttctcctga
    33361 gaattccggc agagaaagca aaaataaatg cttgactctg tagcgggaaa gcgtattatg
    33421 cacaccccgc gccgctgaga aaaagcgaag cggcactgct ctttaacaat ttatcagaca
    33481 atctgtgtgg gcactcgaag atacggattc ttaacgtcgc aagacgaaaa atgaatacca
    33541 agtctcaaga gtgaacacgt aattcattac gaagtttaat tctttgagca tcaagctttt
    33601 aaattgaaga gtttgatcat ggctcagatt gaacgctggc ggcaggccta acacatgcaa
    33661 gtcgaacggt aacaggaaac agcttgctgt tt
//
LOCUS       DAZFFG010000039        33552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFFG010000039 DAZFFG010000000
VERSION     DAZFFG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.039"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1447
                     /gene="citT"
                     /locus_tag="ACHJ7J_002233"
     CDS             <1..1447
                     /gene="citT"
                     /locus_tag="ACHJ7J_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO5000465.1"
                     /translation="NIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMIL
                     EPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVF
                     GAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGT
                     VFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVS
                     KIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGA
                     LTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWN
                     TLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASL
                     SAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVK
                     SKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            1561..2367
                     /gene="rna"
                     /locus_tag="ACHJ7J_002234"
     CDS             1561..2367
                     /gene="rna"
                     /locus_tag="ACHJ7J_002234"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO5000466.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            2596..3006
                     /gene="rnk"
                     /locus_tag="ACHJ7J_002235"
     CDS             2596..3006
                     /gene="rnk"
                     /locus_tag="ACHJ7J_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO5000467.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            3144..3233
                     /gene="yldA"
                     /locus_tag="ACHJ7J_002236"
     CDS             3144..3233
                     /gene="yldA"
                     /locus_tag="ACHJ7J_002236"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO5000468.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(3237..4475)
                     /gene="ybdR"
                     /locus_tag="ACHJ7J_002237"
     CDS             complement(3237..4475)
                     /gene="ybdR"
                     /locus_tag="ACHJ7J_002237"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO5000469.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPLGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAIPGGTI"
     gene            4695..5123
                     /gene="uspG"
                     /locus_tag="ACHJ7J_002238"
     CDS             4695..5123
                     /gene="uspG"
                     /locus_tag="ACHJ7J_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO5000470.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(5244..6809)
                     /gene="ahpF"
                     /locus_tag="ACHJ7J_002239"
     CDS             complement(5244..6809)
                     /gene="ahpF"
                     /locus_tag="ACHJ7J_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO5000471.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(6938..7501)
                     /gene="ahpC"
                     /locus_tag="ACHJ7J_002240"
     CDS             complement(6938..7501)
                     /gene="ahpC"
                     /locus_tag="ACHJ7J_002240"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO5000472.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            7873..8619
                     /gene="dsbG"
                     /locus_tag="ACHJ7J_002241"
     CDS             7873..8619
                     /gene="dsbG"
                     /locus_tag="ACHJ7J_002241"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO5000473.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLKVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            8828..9730
                     /gene="ybdO"
                     /locus_tag="ACHJ7J_002242"
     CDS             8828..9730
                     /gene="ybdO"
                     /locus_tag="ACHJ7J_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5000474.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNECF"
     gene            9877..11097
                     /gene="ybdN"
                     /locus_tag="ACHJ7J_002243"
     CDS             9877..11097
                     /gene="ybdN"
                     /locus_tag="ACHJ7J_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5000475.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            11070..11699
                     /gene="ybdM"
                     /locus_tag="ACHJ7J_002244"
     CDS             11070..11699
                     /gene="ybdM"
                     /locus_tag="ACHJ7J_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5000476.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(11700..12860)
                     /gene="ybdL"
                     /locus_tag="ACHJ7J_002245"
     CDS             complement(11700..12860)
                     /gene="ybdL"
                     /locus_tag="ACHJ7J_002245"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO5000477.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            12969..14057
                     /gene="hcxA"
                     /locus_tag="ACHJ7J_002246"
     CDS             12969..14057
                     /gene="hcxA"
                     /locus_tag="ACHJ7J_002246"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO5000478.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNNAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(14067..14264)
                     /gene="ybdD"
                     /locus_tag="ACHJ7J_002247"
     CDS             complement(14067..14264)
                     /gene="ybdD"
                     /locus_tag="ACHJ7J_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5000479.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(14446..16551)
                     /gene="cstA"
                     /locus_tag="ACHJ7J_002248"
     CDS             complement(14446..16551)
                     /gene="cstA"
                     /locus_tag="ACHJ7J_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO5000480.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(16732..17145)
                     /gene="entH"
                     /locus_tag="ACHJ7J_002249"
     CDS             complement(16732..17145)
                     /gene="entH"
                     /locus_tag="ACHJ7J_002249"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO5000481.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(17148..17894)
                     /gene="entA"
                     /locus_tag="ACHJ7J_002250"
     CDS             complement(17148..17894)
                     /gene="entA"
                     /locus_tag="ACHJ7J_002250"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO5000482.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQVSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(17894..18751)
                     /gene="entB"
                     /locus_tag="ACHJ7J_002251"
     CDS             complement(17894..18751)
                     /gene="entB"
                     /locus_tag="ACHJ7J_002251"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO5000483.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMAQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(18765..20375)
                     /gene="entE"
                     /locus_tag="ACHJ7J_002252"
     CDS             complement(18765..20375)
                     /gene="entE"
                     /locus_tag="ACHJ7J_002252"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO5000484.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     HFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLITKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(20385..21560)
                     /gene="entC"
                     /locus_tag="ACHJ7J_002253"
     CDS             complement(20385..21560)
                     /gene="entC"
                     /locus_tag="ACHJ7J_002253"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO5000485.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQLLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            21749..22705
                     /gene="fepB"
                     /locus_tag="ACHJ7J_002254"
     CDS             21749..22705
                     /gene="fepB"
                     /locus_tag="ACHJ7J_002254"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5000486.1"
                     /translation="MRLAPLYRNALLLTGLLLSGISAVQAAGWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(22709..23959)
                     /gene="entS"
                     /locus_tag="ACHJ7J_002255"
     CDS             complement(22709..23959)
                     /gene="entS"
                     /locus_tag="ACHJ7J_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO5000487.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            24070..25074
                     /gene="fepD"
                     /locus_tag="ACHJ7J_002256"
     CDS             24070..25074
                     /gene="fepD"
                     /locus_tag="ACHJ7J_002256"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO5000488.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            25071..26063
                     /gene="fepG"
                     /locus_tag="ACHJ7J_002257"
     CDS             25071..26063
                     /gene="fepG"
                     /locus_tag="ACHJ7J_002257"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO5000489.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            26060..26875
                     /gene="fepC"
                     /locus_tag="ACHJ7J_002258"
     CDS             26060..26875
                     /gene="fepC"
                     /locus_tag="ACHJ7J_002258"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5000490.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSIANS"
     gene            complement(26872..28005)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ7J_002259"
     CDS             complement(26872..28005)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ7J_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO5000491.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANIKDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRHAMASRKQDAMMADHLV"
     gene            complement(28221..32102)
                     /gene="entF"
                     /locus_tag="ACHJ7J_002260"
     CDS             complement(28221..32102)
                     /gene="entF"
                     /locus_tag="ACHJ7J_002260"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO5000492.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRCQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLAMQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSARRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(32099..32317)
                     /gene="ybdZ"
                     /locus_tag="ACHJ7J_002261"
     CDS             complement(32099..32317)
                     /gene="ybdZ"
                     /locus_tag="ACHJ7J_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO5000493.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(32320..33522)
                     /gene="fes"
                     /locus_tag="ACHJ7J_002262"
     CDS             complement(32320..33522)
                     /gene="fes"
                     /locus_tag="ACHJ7J_002262"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO5000494.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLEAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
BASE COUNT         8023 a         9216 c         8461 g         7852 t
ORIGIN      
        1 taatatatgg aaactattgg ccccactggt ggtgatgggc gtcatgtttc ttatccctgt
       61 ccccgacggt atgccgccgc aggcatggca ttacttcgct gtgtttgtgg caatgattgt
      121 cggcatgatc ctcgagccaa ttccggcaac agcgatcagt tttattgcgg ttactatttg
      181 cgttattggc agtaattacc tgctctttga tgccaaagaa ttagctgacc cagcgtttaa
      241 tgcgcaaaaa caggcgctga aatggggtct ggctggtttt tccagcacca cggtatggct
      301 ggtatttggc gcatttattt ttgcattagg gtatgaagtt tccgggttag gtcgtcgcat
      361 tgcccttttc ctggtgaaat tcatgggcaa acgcacgctg acgttgggtt atgcgattgt
      421 cattatcgac attctgctgg caccgtttac accgtccaac accgcgcgta ccgggggtac
      481 ggtttttccg gtcattaaaa acctgccgcc gctgtttaaa tcattcccga acgatccgtc
      541 cgcgcgtcgt attggcggct atttgatgtg gatgatggtc attagtacca gtctgagttc
      601 gtccatgttt gtcaccggtg cggcaccaaa cgtgctgggt ctggagttcg tcagcaaaat
      661 tgccggtatc cagattagct ggttgcagtg gttcctctgc ttcctgccgg ttggggttat
      721 cttgcttatc attgcgccgt ggctttccta cgtgctgtac aaaccggaaa tcacacacag
      781 tgaagaagtg gcaacctggg cgggtgatga actaaaaacc atgggtgcgc tgacacgcag
      841 agagtggacg ctgattggcc ttgtattgct cagcttaggt ttgtgggtat ttggcagtga
      901 agtcattaat gctactgcgg ttggtctgct ggcagtttcg ctaatgctgg ccctgcacgt
      961 tgtgccgtgg aaagacatta cccgctataa cagcgcatgg aacacgctgg tcaacctggc
     1021 aactctggtt gtgatggcta acggcctgac tcgttctggt tttattgact ggttcgccgg
     1081 taccatgagt acgcacctgg aaggattctc accaaacgca acggtgattg tactggttct
     1141 ggtgttctac tttgcacact acctgtttgc cagcctgtct gcgcacaccg caaccatgct
     1201 gccggttatt ctggccgtcg gtaaaggtat tccgggcgta ccaatggaac aactgtgtat
     1261 cctgctggtg ctgtctattg gtataatggg ctgtctgacg ccgtatgcaa ccggtcctgg
     1321 ggtgattatt tacggctgtg gctatgtgaa atcaaaagat tactggcgtc ttggcgcaat
     1381 cttcggggtg atttacatct ctatgttgct gttggttggc tggccgattc tcgccatgtg
     1441 gaactaatac gttcgccaaa cgctgaataa atcatcacgc ccgccaggta agcccacctg
     1501 gcgggctttt ttatgattta atagatagta ttcagtgtat gtatgagttc cacacccatt
     1561 atgaaagcat tctggcgtaa cgccgcgttg ctcgcggttt ctctgcttcc cttctcttct
     1621 gccaacgccg tagcgttgca ggcaaaacag tatggcgatt ttgatcgcta tgtcctggcc
     1681 ctctcctggc aaaccggatt ttgccagagt caacacgatc gaaatcgtaa cgaacgagat
     1741 gaatgtcgcc tgcaaaccga aacgaccaac aaagctgatt ttctgaccgt acatggtctg
     1801 tggccaggat tgcctaaatc ggtcgctgcc cgtggtgttg atgaacgccg ctggatgcgc
     1861 ttcggttgcg ctactcgccc aatcccgaat ctaccagaag cgcgcgccag ccggatgtgt
     1921 tcatcgccgg aaaccggatt atcactggaa acggccgcta aactaagtga agtcatgcca
     1981 ggagctggcg gacgttcctg cctggaacgc tacgaatatg ccaaacacgg tgcctgcttt
     2041 ggttttgatc cggacgcata cttcggtacg atggtacgcc tgaatcaaga aattaaagag
     2101 agcgaagccg gaaaattcct tgcggataat tacggtaaaa cagtgagccg ccgtgacttt
     2161 gacgccgcct ttgccaaaag ctggggaaaa gagaacgtga aagcagttaa gctaacgtgc
     2221 cagggtaacc ctgcgtattt gactgaaatt cagatctcga tcaaagctga cgccatcaac
     2281 gctccgcttt ctgcaaactc attcttgcca caacctcacc caggtaactg tggcaaaacc
     2341 tttgtgattg ataaagcggg ttattaatcc tctgatttac aaaacacgct aattcgtctt
     2401 ccataaccat gacgattaag aattatacat aatgcgctgc attgaggaat tccctgaatg
     2461 tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag ttaaaccctc
     2521 gccgcctgac ggtgagggtt ttcttttggg tatgtttatc tgcgacactc gcagtaccga
     2581 cgacatggag taaaaatgtc cagaccaact atcatcatta acgacctgga tgccgaacgc
     2641 atcgatattc tgctggagca acccgcctat gctggtttgc caatcgccga cgcgttaaac
     2701 gcagagttgg atcgcgccca aatgtgttcg ccagaagaga tgccacacga cgtggtgaca
     2761 atgaacagcc gggttaaatt ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg
     2821 gtgtatccgg caaaaatgac cgatagcaat actcagcttt ccgttatggc tccggtaggt
     2881 gccgcactgc tggggctgcg cgttggcgat tccattcact gggaacttcc gggcggcgtt
     2941 gcaacccacc ttgaagtgct ggaactcgaa taccagccag aagctgctgg cgactatctg
     3001 ctttaatccc atctgcacaa gcgataactc gctttgccca gaggatgcac tcgcatcctc
     3061 tttacgcaaa atcagcaatt tttcctggtt ctcactgttc acttttgctg caaaattatt
     3121 ccgtttcgtt tgtgggaata accatggcag aagcatttta catcctgata ggatttctga
     3181 ttatggcggc gattatcgtc atggctgtcc tttacctcga aaaccactcc tgacgatcat
     3241 attgttcccc ccggaatcgc attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg
     3301 ctttgtgcac cgggtaccag aatcacctta cggcactcct cttcacgttt ttcgaaaatc
     3361 tcatatcccc gggcggcctc ttcaaacggc atatagtggg taacaatttc ttctggtttc
     3421 agtaatcctt tctcaattaa cggtaataat tctcccagcc atgcgtgaac gtgggtctgt
     3481 cccattttaa acgtcaaccc tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat
     3541 ccagcgtaga cgcccggtac gctaacaatg ccgccacgcc tgaccgccgc aatacactgg
     3601 cgcaacgctt taccgctgct gccctccagt ttcaggttag tcagcaccgt ttccgtggtg
     3661 ctgcctttcg cttcaaaacc gacggcgtct attactgcat ccacgccccg gtgacctgcc
     3721 gtttgttcaa taattgactg tgccggatcg ctatcttcat cgaaattaat cgggatcgcg
     3781 ccgtagcggt cagcggcgaa acgcaagcgg tagggatgat gatcaacaac aaaaatctgt
     3841 tccgcaccga gcaaccgtgc acaggcgatt gtcaacaatc ccaaaggacc ggcaccatag
     3901 actgcaacgc ttgaaccttg ttggatctgc gcattttttg ctgcctgcca tgccgttggc
     3961 agaatatcag aaaggaaaag cgctttatca tctgaaagca aaggcggtac tttaaacggc
     4021 cccacattcc ctttagggac gcggacatat tccgcctgcc cgccaggaac gccaccatac
     4081 aggtgactat aaccaaacaa tgccgcggga gcaggtatct gttttttatt gagcgcagcg
     4141 cctttacccg cattggtatt ttcgcaggcg gcatattgct gcattcgaca gaaaaaacag
     4201 tcgccacaag caatgacgaa cggaattacc acccggtcgc ctttttgcaa attttttacg
     4261 tccttcccgg tttcaactac ttcccccata aattcatgac caaaaatatc gccatgttta
     4321 acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttgccgta
     4381 atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga
     4441 acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa
     4501 aagtcagtct ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg
     4561 agaattcctg gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga
     4621 accagacaaa tatcagggat aaatattata atcatggcga atgatttgtt catgattaac
     4681 agggagaaag ggttatgtat aagacaatca ttatgccagt tgatgtattt gaaatggaat
     4741 tgagcgacaa agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc
     4801 tacttcacgt actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc
     4861 gtcgttttga agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc
     4921 acttcaccat cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg
     4981 aagtcaatga gttggcagaa gaactggggg ctgatgttgt agttattggt tctcgcaacc
     5041 catcaatttc gacccatctg ttaggttcta acgcctcgag cgtgatccgc cacgccaatc
     5101 tgccggtgct ggttgtacgt taattgtcag aataagtctc cagccctgcc cggagccatc
     5161 ccggcggggc tttttattgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa
     5221 ttgcaggtga atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca
     5281 ctcagagagg ctttggcacc ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc
     5341 gtacagtcac ccgctgcgaa cacgcctttg acgttggttt cgcatttcgc atcaatgata
     5401 atctcgccca tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga
     5461 ccaatctgga caaaaatacc ggccagttcg atgttgtgaa tatcgccgct gacacgatct
     5521 cgatattcca gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc
     5581 agaataatgt cgacgttttt caggctgcgc agtttgtcct gcagcacctg gtcggctttc
     5641 atttctggcg caaattccag cagagttacg tgctcaacga tacccgccag gtcgattgcc
     5701 gcttccacgc cggagttacc gccgccgata accgctacgc gtttgccttt aaacagcggg
     5761 ccgtcgcagt gcgggcagta ggtcacgcct ttggtgcgat actgatcttc gcccggaacg
     5821 ttcatgttgc gccattttgc accggtcgcc acgataatgc tgcgtgcttt cagtaccgcg
     5881 ccagaagctg tttcaatctg atgcagacca ccttcaactg ctgctgggat cagtttgctg
     5941 gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct
     6001 gccaacttct gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg
     6061 aggatctgac caccaaaacg ttcgcccatc agaccagtac ggatgccttt acgtgcggag
     6121 taaattgctg ccgctgcacc cgccgggccg gaaccaacga ttaatacgtc ataagcatca
     6181 cgcttgttca gctcttctgc cgcacgtttt tccgcgccag tatcaatttt ggcaacgatt
     6241 tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg
     6301 cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc
     6361 ttgatgcgcg ggttcaatac gctcatcagg ttcagcgcct gcaccacgtc cgggcagtta
     6421 tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc
     6481 tccagcagag actgcgcttc tttcgacgga tgaccaccgg tccacagtaa cgccagtacc
     6541 agcgaggtga actcgtggcc cagcggggag cctgcaaaac gtggcccctg gttagaacct
     6601 gggttggtga tcaggaaaga cggcttacgc accggcaagc tgttatcttc tttaaaggtg
     6661 actttgtctg acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg
     6721 ctgtcatcca gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg
     6781 agttgagttt tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg
     6841 caagcggcct ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg
     6901 gtgccgcagc acccgattaa ttacgcgatt gcggaactta gattttacca accaggtcca
     6961 gagacggagc cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt
     7021 gagaagctac gtactgtgct gctttgattt tacgcagcag gtcagacgcg tcacggccaa
     7081 tgccttcagc ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg
     7141 cacggtcagc cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag
     7201 tcgggtcgcc gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg
     7261 ctttgtgggt gaagtgagta tcggtagata ctgcgtatac gtctacgccc agtttctgca
     7321 gttcttcgta gtggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag
     7381 ccgggtagaa gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga
     7441 attcgccgtt tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca
     7501 tctatacttc ctccgtgttt tcgatgagat gtaaggtaac caatttttgc cgattcggct
     7561 aatgcgtttc cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg
     7621 tgaactttgc accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg
     7681 taatctgaac taccctttgg tatcttcagc gcctgtctgc aggctgcggt gttgctctta
     7741 caaatgcaac aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct
     7801 aagctcttac ctatgacaat gataggttat gccttttact cgacttttgc actgactgaa
     7861 aaggacaaat taatgttaaa aaagatacta ttactggctc tgcttcctgc aatcgccttc
     7921 gcagaggaac ttcctgctcc agtaaaagcg attgaaaaac agggtattac gatcatcaaa
     7981 acgttcgatg cccccggagg aatgaaaggt tatctcggaa agtatcagga tatgggcgtc
     8041 accatctacc tgactccaga tggtaagcac gctatctctg gttacatgta caacgagaaa
     8101 ggtgaaaacc tgagtaacac acttatcgaa aaagaaattt acgcaccagc cggacgcgaa
     8161 atgtggcaac ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga cgcgccggtc
     8221 attgtctacg tcttcgccga tccgttctgc ccatattgta aacagttctg gcagcaggcg
     8281 cgcccgtggg tagattctgg caaagtacag ttgagaacat tgttggttgg ggttatcaaa
     8341 ccagaaagtc cggcgacagc cgcggcaatt cttgcctcca aagatcccgc aaaaacctgg
     8401 caagaatatg aagcctctgg tggcaagctt aagctaaagg tgcctgcaaa cgtaagtaca
     8461 gagcaaatga aagtgttaag cgacaacgag aaactcatgg acgatctggg ggcaaatgtc
     8521 actccggcta tctattacat gagtaaggaa aatacgctgc aacaggccgt ggggttgccc
     8581 gatcagaaaa cgcttaatat cattatggga aataaataaa aggtaattca tggatggttg
     8641 aactatatcc ctgcttttca accatcctaa aaaagcatga attgctcgct ctgccctaag
     8701 tggctgtgct attttagaaa aacttaatcc tgagttaagc aaaaaaacat aatcaataaa
     8761 aatatatacg caaaaaacat tgattaagtg aatatatcat ggaagaaaaa tataaccgga
     8821 gtagtgtatg accaatctct acgacttgaa aaagttcgac ttaaatcttc ttgtcatatt
     8881 tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat atatcacccc
     8941 atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc ctttattcat
     9001 tcgctcagga aaaggtatcg cgccgacgac aacagggtta aatctgcatc accatcttga
     9061 gaaaaatctc agaggccttg aacaaacaat taatatagtc aataagtccg aactaaagaa
     9121 aaactttatt attcacggcc ctcagctcat ttcttgctct aataatagta tgctgatccg
     9181 ctgcctacga caagattcct ctgtcgagat tgagtgccat gatatcctca tgtcagcaga
     9241 gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt
     9301 gatcagccgt tccgtcattt gcatgccatt gcatacgatt cgcaatactc taatttgtag
     9361 taataggcat ccacgaatta ctgacaatag cacttatgaa caaattatgg cagaagagtt
     9421 cacccaatta atatctaaat cagcaggggt tgatgatatt caaatggaaa tagacgagag
     9481 atttatgaat cgcaaaattt catttcgtgg ttcctctttg ctgacaatca ttaatagcat
     9541 tgcagttaca gatttactgg gaatagtccc ctatgaactt tataactccc accgtgactt
     9601 tcttaattta aaagagataa aactagaaca tccgttacct tcaataaagc tttacatttc
     9661 ctataataaa tcttctttaa acaatctggt gttctcccga tttattgacc gtctaaatga
     9721 gtgtttttga tatataacct cagacagcta ctataacttt cacctattta ttcacagcgc
     9781 gaatgaaaaa taaaacactc tgcttttcag tgtttacttt ataattaaca gtcttatctc
     9841 actgatcacc ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt
     9901 ccgctcaata ttctggaagc tgcacgagag cgtattacct ggacactcaa caccctaccc
     9961 cgcgtttgtg tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc
    10021 gaacttgccc gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggcgcaa
    10081 ttctcttgca ccattaacta tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa
    10141 gagttttact gggttgcgct cccgcttacg acgcaaaatt ccctttcaca ataccaaccc
    10201 gaatggcagt gctgggaacc tgatgtcgaa tgggtgcgtc agcccccgca agatgcgata
    10261 accgatcctg actttttctg cttttaccag ccaggcatga ccttcgaaca atttgtacgt
    10321 gagtttgccg aatggttttc acaaaaacgt ccggcggcga tgatgatcgg catccgtgcg
    10381 gatgagtcct acaaccgttt tgtcgccatc gccagtttaa ataaacaacg ttttgccgac
    10441 gataaaccct ggactacggc cgcaccaggc ggtcatagct ggtacattta ccccatttac
    10501 gactggaaag tggctgatat ctggacctgg tatgcaaatc atcaaagtct ctgcaaccca
    10561 ctgtataacc taatgtatca ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct
    10621 tttggcccgg agcaacgaca aggattatgg ctctatcacg ttatcgaacc ggatcgctgg
    10681 gctgctatgt gcgcacgagt cagcggggta aaaagtggcg gcatttacgc cggacatgac
    10741 aatcatttct atgggcatcg gaaaatcctc aagccagaac atttagactg gcaagaatat
    10801 gcattattgc tgctcaatag catgccggaa aaaacagctg agcattaccg caataaaatc
    10861 gccatttatt tgcactggta tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg
    10921 gacattggcg cgaaagatat cccctcctgg cggcggatat gcaaagtttt actcaataac
    10981 gattactggt gtcgagcatt atcatttagc cctactaaag cgaagaacta tcagcgttat
    11041 aacgaacgga taaaaggaaa acgtcaggaa tgggggatac tatgcaacaa cgattaacgc
    11101 aggatttaac gcaatttctc gccagcttgc cggaagatga tcgcatcaaa gcgattaatg
    11161 aaatccgcat ggcgatccat caggttagcc cctttcgtga agagccagtg gattgcgttc
    11221 tgtgggttaa aaacagccaa cttatgccta atgattacaa cccaaacaac gtggcaccac
    11281 ccgaaaaaaa actgctgaaa aaatccattg aaatagatgg ttttacccaa cccattgtgg
    11341 tcacgcatac ggataaaaat gccctggaaa tcgtggatgg ttttcaccgc catgaaattg
    11401 gtaaaggctc atcatcgtta aaattacgct taaaaggtta tttgccggtg acctgtctgg
    11461 aaggtacgcg taatcaacgc atcgccgcga ctatacgtca taaccgcgcc cgtggacgcc
    11521 accaaatcac cgccatgtca gaaatcgtcc gtgaacttag ccagttggga tgggacgata
    11581 ataaaattgg caaagagctg ggaatggaca gcgacgaagt gctgcgcctg aagcaaatta
    11641 acggtctgca agagctgttt gccgaccgtc aatattcccg cgcctggacg gttaaatagc
    11701 taaagctggc gcaggcgttc agctgctgcc agcaacgtcg attccttctt ggcaaaacag
    11761 agacgaatca gtttatgtgg gaagggatcg gcgcaaaaca ccgatagcgg aatcgccgcc
    11821 acgccgtgct cccgcgtcag ccactggcaa aactcaacat catccagggt agaaaccgcg
    11881 ctgtaatcca ccagcaaaaa gtatgtgcct tcgcacggta aaatctccag ccgactttca
    11941 tttaaggcat ttaccagaat atcgcgcttc tggcgataaa agtccggtaa cgcaagataa
    12001 tgctcaggtt ctgcacgtag catatccgca attgccagct gtgccggggt attcaccgaa
    12061 aaggtcagat actgatgtac cttgcgaatt tcggcactga tgggcgctgg cgcaacacaa
    12121 taacccactt tccagccggt catatgatag gtcttgccaa acgatgacac cgccaccgcc
    12181 cgctcacgca gctgcggatg cgccagcaca ctggcatggc cctgttgtga aaagttgatg
    12241 tgctcgtaga cttcatcgct aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa
    12301 gcggcgaaat cagcctgctg ccagacagtt gcactgggat tatgcggagt gttgaggatc
    12361 accagtctgg tgcgctcgct taacaatgcg gcaaattcct gccagtcaac gcgaaaatgc
    12421 ggtggttgca gtgccatacg cttcactatt cccccagaaa gcgcgatggc gggggcgtaa
    12481 ctgtcatagc tgggatcaaa acaaatcact tcatcgccat tgcgcaccag tgcggtaatc
    12541 gccgcgtata acgcttccgt cgcccctgcc gttacggtga tatcgctatc ggcatctggt
    12601 tgatagccat acaaacgttc cgttttctga gcaatcgcct cgcgcaaggc ctgcacgccg
    12661 gtcatgggcg cgtattggtt tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa
    12721 tagcgcggac catcaaaatc aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc
    12781 gccagcgcgc tcatctgggt gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga
    12841 atcagagggt tatttgtcat ttttattgta cctgacgaag ttgcgtgttg aaggcactat
    12901 aacacgatgc tagtatttgg caatcaagac gtttagatgt ctaaataaaa caataaggac
    12961 aacacaacat gcctcacaat cctatccgcg tggtcgtcgg cccggctaac tacttttcac
    13021 atccaggaag tttcaatcac ctgcacgatt ttttcactga tgaacaactt tctcgcgcgg
    13081 tgtggatcta cggcgtacgc gccattgctg cggcgcaaac caaacttccg ccagcgtttg
    13141 gacttccagg ggcaaagcat attttgtttc gtggtcattg cagcgaaagc gatgtacagc
    13201 aactggcggc tgagtccggt gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac
    13261 tactcgacac cgcgaaagcc ctcgcccgcc gtctcggtct gccatttgtt gccgttccga
    13321 cgatcgccgc cacctgcgcc gcctggacac cgctctccgt ctggtataac aatgccggac
    13381 aggcgctgca ttatgagatt ttcgacgacg ccaattttat ggtgctggtg gaaccagaga
    13441 ttatcctcaa tgcgccgcaa caatatctgc tggcggggat cggtgacacg ctggcgaaat
    13501 ggtatgaagc ggtggtgctg gctccgcaac cagaaacgtt gccgctaacc gtgcgactgg
    13561 ggatcaataa tgcgcaggcc attcgcgacg tcttgttaaa cagtagcgaa caggcgctga
    13621 gcgatcagca aaatcaacag ttaacgcaat cattttgcga tgtggtggat gctattattg
    13681 ctggtggtgg gatggtcggt ggtctgggcg atcgttttac gcgtgtggcg gcggcccatg
    13741 ccgtgcataa cggtctgact gtcctgccgc aaaccgagaa gtttctccac ggcaccaaag
    13801 tcgcctacgg aattctggtg caaagcgcct tgctgggtca ggatgatgtt ctggcgcaat
    13861 taactggagc gtatcagcgt tttcatctgc cgactacgct ggcggagctg gaagtggata
    13921 tcaataatca ggcggagatc gataaagtga ttgcccacac cttgcgcccg gtggagtcca
    13981 ttcattacct gccagtcacg ctgacaccag atacgttgcg tgcagcgttc gaaaaagtgg
    14041 aatcgtttaa agcctgacgt gcaaatttag cagcagcgcg cgccgccttt tccaccgtag
    14101 cgcgcgtcct gccgctcacg gaaaaactct tcgtaggtca tcggcgtttg atcgggatgg
    14161 ttaacccgca tatgttcgac atagttgtcg taatcaggca taccaatcat cagcttcgcc
    14221 gcctgaccta aatattttcc ggcttttgcc agtgaatcaa acatcttggc ctccgctaac
    14281 catgttgctg aaagagtgcc ggatgcggcg tgaacgcttt atccggccta caaatgctat
    14341 caacgcgctc ggtcagtccc ctcgccccat cggggagagg gttagggtga gggggaaccc
    14401 tcggcactat gccaacattc gccaacatcc cccctcactc tgactttagt gcgcgccttt
    14461 cgcctgcgct acgatctcct caacattttc cggcatcggc tcatacggcg tttctttcgc
    14521 cgttggcttc ggatctttca atgccgcaag tgccgtctta atcgagaaca gtgccagaac
    14581 caccacgacc accataaaga agatggttaa cccggcatcc agacggttgt tgaacaccag
    14641 ttgcgccagc tgtgactcag tatactgcga cggaatattg ccgctgtcga tcattgcctg
    14701 gaatttgtta gcaatggcca ggaagccgac tttcgcatcc gggctaaacg ctttctgcca
    14761 gccagcggtc agggtacaaa tcagcagcca ggccgttggt accagcgcca cccaggcgta
    14821 acgttgacgt ttcatcttga acaacaccac ggcacagagc atcagcgcca tccctgccag
    14881 catctggttg gcaataccaa acagcggcca cagagtgtta ataccgccca gcggatcgac
    14941 cacaccctga tgcaggaagt agccccacgc cagcacgcac agcgccgttg ccagcaggtt
    15001 agcaggcagt gaatcggtcc gtttcaggcc cggagaaacc acgcccagca gatcctgcaa
    15061 cataaagcgc gcagcacgcg tacctgcatc caccgccgtc agaataaaca gagcttcaaa
    15121 cagaatggcg aagtgatacc agaacgccac atccatcata ccacccagcg caccgtgcag
    15181 gatgtaagcc atccccaccg ccagcgtcgg cgcaccgccc gcacgggaaa tgatcgactg
    15241 ttcacccact tcgctggcaa tctggttgag cgtatccgga gtaatcgcaa agccccagct
    15301 actcaccact tgcgcggcag aagcgaccac atccgccgtc cctgccggag ccagcaccgc
    15361 catcgggctg ttcatggcaa agtacacgcc cggatcgatg atacaggcgg aaaccagtgc
    15421 cataatcgcc acgaaggatt ccatcaacat cccgccgtag ccgataaagc acgcctgccc
    15481 ttcgttcgcc agcatcttcg gcgtggtacc ggaagagatc agcgcatgga agccagacac
    15541 cgcaccacag gcgatggtga taaacaggaa cgggaacagg ttaccggtcc ataccgggcc
    15601 agtgccatcg acaaatttgg tcagcgcagg catggtcagc gtcggacgca taatcaaaat
    15661 gcctaccgcc agaccgacga tagtcccgat tttcaggaag gtagagaggt agtcacgcgg
    15721 ggccagcagt aaccacaccg gcagcaccgc cgccacaaaa ccgtagccca ccagcatcca
    15781 ggtcagctgc acgccggtaa agtcaaagta cggtgcccag gtcgggcttt ctgccaccca
    15841 gccgccagag ataatggcaa aaatcaggaa caccaggcca atcactgaca cttcgccaat
    15901 acgccccgga cgcagatagc gcaggtagat tcccataaaa atcgccagcg gaatggtgaa
    15961 cgcaacggta tacgttcccc acgggctatg agtcagggct ttcaccacga tcatcgccag
    16021 tactgcaagg ataatgacca tgatcataaa gcaggccacc agcgcgatca ccccggcggt
    16081 tggccccatc tcttctttga ccagctcacc cagcgaacga ccgtcacggc gcgtagaaac
    16141 aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc accccggcga gtagccagat
    16201 catccctggc aggtaaccca tttgcgccgc cagtaccggc ccaaccaacg gacctgctcc
    16261 ggcaatggcc gcaaaatggt gaccgaacag cactttcttg tccgtcggca catagtccag
    16321 cccgtcgtta tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt
    16381 ggcgatatac aggccataaa aacggtaagc gatcagatag atacagaccg acgccaccac
    16441 aatccacagc gcgttgatct gttccccacg atttaaagca atgtatccca gagcaaatgc
    16501 tcccattaca gagagcactg tccagacgag gtatttccct gatttgttca tagttgttat
    16561 ccgtgtgcgt gtccatagag atgttacatt ttgtttctat aacatctttg tgcgatttaa
    16621 caacacggta accatgtaaa tcggagttga aaccaatatt taacttaaca atgttaactc
    16681 gatcactcca ttaaccgagt tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa
    16741 aactgccgta cccagccgac aagtgcagca acgccgcccc tgttcatcga aaacgacgat
    16801 ttcccagctc tgattttgcc gcccaagatg cagcggctgg cagacgccgc gtaccttgcc
    16861 ttcagacacc gggcgatgat gggtggcgtt aagttccgtg cccaccacac actgcccgtc
    16921 acgggtcatc ataaatccgg ccatcgatcc cagcgtttca gccagcgccg ccgacgcgcc
    16981 gccatgcagc aggccaaacg gctgatgagt acgggtatca accggcattt cggcttccag
    17041 cacatcatcg cccagacggg tatacacaat tcccagatgc gccaccattg tgttatcgct
    17101 ggtggcgttc agttcgtcga gcgttaaatg gcgtttccag atcatgctta tgcccccagc
    17161 gttgagccgc catcgaccac aatatcctgt agggtgatat ggctggcgag gtcagaggcg
    17221 aggaacaaaa tcgtgttggc gatctcttgt ggacgggcga ttttccccag cggaatgccg
    17281 agtttaaact gctcgccaaa gccgcgaata cgctgttctt cggcgtcatc gctcacccac
    17341 agcgtgcgtt gcatatcggt gtcggtggag ccaggcgaaa ccacattaca gcgcacgccg
    17401 ctacccgcca gttccagccc gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg
    17461 ccataagcac tcataccaat acgcggcgtg tgtgcggcgt cggacgccac agtgacaatc
    17521 gccccgcccc gctgacggcg aaactggttc atggtttgct gaaacaggtt aaacgcaccg
    17581 ccgacgttaa ccgcaaaagt ctgctgccag tcctctttgc tcacttggtc tgtcgcgccc
    17641 atgcgtaaaa ttcccgccgc attgaccagc gcgtccagtc gctccgtttc agctaacagt
    17701 cgctgacaca cttgcgcgac ctgcgcagcg tcggcaacat ccatcacttc ggtcgcaaag
    17761 ggatattgct cctgagcgaa cgcttgatca aaacctgtaa ctttcgctcc cgcttcaaca
    17821 aacgccagcg ccgtggcgta gccgatacct ttccccgcgc cggttaccca gacattttta
    17881 ccgctgaaat ccattatttc acctcgcggg agagtagctt ccaccaggcg tcgatggtcg
    17941 ggtttttcgc cagcatgaca aagtcgatat cgccatgcac tttgcgccag cgcgccgcca
    18001 gcgccatcat gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt
    18061 catcggactc gtccagcaac ggcaggatca cctcacgcag cgccgctttg ctggcgggga
    18121 taggtgctgg cagtaattct tcggtcatca ccacccggcc agaacgtccg gccacatatt
    18181 tcagcgacat caaatgctcg tcacggctga aatcagccag cgcatccgcc accataaacg
    18241 gtttaatatc gcgcataaat gcgtcggttg cggtggtcat acagccaatg tgggcatata
    18301 ccccggtaat aatcagctgg ttacgtccac tctctttgag catttgctcc agcggagaac
    18361 gatgaaacgc gctgtagcgc cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc
    18421 gatccaccac cttttgctgt tccggcgaac gggtcaggcc cggcccccac atatcattca
    18481 acagcgccct atcttcatcg ctttgctctt tcggctgggc ggtgtaataa accgggatat
    18541 tgtgctgttt gcagtagtcg cgcagcgcag caatattcgc gatcacctgc gccatcatcg
    18601 ggcagttctc accccagaag ctgacaaaat agtcctgcat atcatggatt aacaacgcgg
    18661 cacgttgcgg ttcaaaggcc caatcgactt tattctgtgg aatatcgtga gactccggca
    18721 atgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc tgatgcgcgt
    18781 gacgccagcc actgacgtaa ttgtttttta tcgactttcc cgaccgccgt cagcggaagt
    18841 gaatccacac actccacgcg atccggtaat ttaaattcgg caataccctg ttcacgcagg
    18901 aaacgacgca cctgcaccgc gcgcaacggc tcttttacca ccagataagc acagcttttt
    18961 tcgcccatca gctcatcttc catgctcacc agtgcggcat agatcaccgc cggatggcgc
    19021 agcaacaggt tttcgatctc ttcggcagcg atcttctcgc cgccacggtt gatctgatct
    19081 ttctcgcgcc cctgcacggt gatgtaaccc tctggatcaa tagagatcag atcgccggaa
    19141 cagtaaaaac cgttggcatc aaaggcgctg gcattgtgct gcgggctttt gtaatagcca
    19201 cggaaggtgt acggcccgcg cgtcatcagg cgtccgactt ccccttgcgg cagtggattt
    19261 ccttcggcat cggcaaccca tacttcatcg tccggacaca ttgggtaacc ctgggtatgg
    19321 ataattttct ccgcgctatc atcaagacgg gtatagttca ccagcccttc cgccatgcca
    19381 aacacctgct gcaactggca gccaatctca gcgggaatac gggccgcaag cgtggcagaa
    19441 agacgtgcgc cgccgacctg taacagtttc agcgaggcaa gctgcgcccg gctttcgcct
    19501 tcggtcagcg cttgcaacca caggctgact gccggcggca ccagcgcggt aacgttaacc
    19561 tgatgttttg taatcaatgg aaagcagagc gtggcgctgg gatcggccgc cagaacaacc
    19621 gttcctccgg caagaaagac gcccagcgat cccggcgaac tcatggcgta gttatgagcc
    19681 gccgggatcg cgcacaggta gcgcgtctgt tgtgtgaagt gacaaatctc gacgctacga
    19741 cgcacgctgt agtagtagtc gttatgagtg cgcgggatca gtttcggtgt gccggtggtg
    19801 ccgccggaaa gctggaaata ggccacttca tcagcaggtg atggcgtggc agtaaaaccc
    19861 tcagccggat ggttaatcgc atcctgcaag ttatgctcac cgctgtcgtt gagcagctgc
    19921 accacgcgaa tggaagaatg ctctgcaaca aatgtattga ggaaatcatc cccgctaaac
    19981 agcgcatgtt ggcgatcggc aatcagcaat gcgggttcaa tctggctggc ataggcgttc
    20041 agttcactac gctgatggct gaacaacgcc agcacaggcg caacgcccag tttcagcagc
    20101 gcgaaaaagg taatatacaa ttcagcgacg ttacccagtt gtaccagcgc ggtttcacca
    20161 ggtttaatgc cctgacggcg taaactacac gcgaggttat ccgccgcctg attcagctcc
    20221 cggtaactca actgtcgctc gccgtcgata accgcgatgc tgtcactcgc agcgtggcga
    20281 gtcagaatgt cggtcagcgg caaatcctgc cagtagcctt tttcccgata gcgacgggca
    20341 aactcttccg gccagcgggt gaatggaatg ctcatcctcg ctccttaatg caatccaaaa
    20401 acgttcaaca tggtagaaag tttgacgcct gtttcgcgcc actcacccaa cggtgacgac
    20461 gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag
    20521 cggatggtca ccacccattc gccgttacct tcgctgtcac accaacccac aatgccgcca
    20581 aacagttcgc ggtcgaacgg ctccagttca gcaataacct gggtcgcggc ctgatgtggg
    20641 aaaccgctca gcgcgggggt cggatgcagc agacaagcca gagtcagtgc gttttcttgc
    20701 gaattcgctt taccttcaaa gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat
    20761 tgtggagagg agggaacgtg taactcacta ctgcgttcgc gcagtacctc tttcatcgcc
    20821 tgagtcacca gttcatgttc atggcgatct ttttctgacg ccagcagacg attacccgct
    20881 tcgcgatcga gcacttcatc cggctgacga cgcgcggaac cggctaacgg aatggagcta
    20941 aaacgctcgc cgtctttacg tagcagcagt tccgggctgg cccccagcag aacgccacca
    21001 tctgccaacg gcacatggaa gttgtaacta accgggtttt gcgcaatcaa ccgttccagc
    21061 aatacgccac tatcaatggc ggcgtcagtg gtgatatcaa tcaaccgtga caacaccact
    21121 ttgtcgacct gcggcgtggc ggtaagtgcg gcggcgcggg caaccatctg ttcaaacgtg
    21181 gtttgttccg gaattgcctg gcgttccacc acattcagca actggctgcg ggtgaaacgg
    21241 cgtgcggaag tttgtttttc ctgacgggag aacgactgcc aggattcggg aatatacagc
    21301 gacgaaggct gacgtggatc gaagggaatc gccccaacca tcaccggatt tttgatgccc
    21361 tgcgctttgg catcggcaaa cagcgcggcg agtttttgct ggaagggact gtcgggcgaa
    21421 tccccgttca cagccggttc atcgaagcgg gcgaaacatc ctgacgtcgt aaaactgcgg
    21481 tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc
    21541 tcagccagtg acgtatccat atcatcctcc acaaaatgat aaaggcttta ataatgatta
    21601 tcatttatat tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt
    21661 aacgcaatct gcaactcggg gttgcatctg gttgtggtta caatgaaaat gagaagcatt
    21721 aacctcatta ataacaggaa gctgatttgt gagactcgcc ccgctctacc gcaacgccct
    21781 tctattaaca ggacttttgc tttcaggaat atccgcagtt caggccgccg gctggccgcg
    21841 tcagattact gacagccgtg gcactcatac cctggaaagc cagccgcagc gtattgtttc
    21901 caccagcgtc accctgaccg gctcactgct ggcgattgat gctccggtga tcgccagcgg
    21961 cgcgaccacg ccgaataacc gcgtcgcgga tgaccagggc tttttacgcc agtggagcaa
    22021 ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg ccgaagccgt
    22081 tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt cggcgctggc
    22141 gctgtacgat cagctttcca ccatcgcccc aacgctaatc atcaattacg acgacaaaag
    22201 ctggcaggcg ctgttaacgc agcttggcga aattaccggg catgagaaac aagcggcaga
    22261 gcggattgca cagtttgata agcaactggc ggcggcgaaa gagcaaatca aattaccgcc
    22321 gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc tctggacgcc
    22381 ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga agttgcccgc
    22441 aggcttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc ttggtgggga
    22501 aaatctggct gcagggttaa atggcgagtc actattcctg ttcgccggtg atcagaaaga
    22561 tgccgatgct atttatgcta atccgctgct cgcgcacctg cctgcagtac aaaacaagca
    22621 ggtttatgcg ctgggaaccg agacgttccg tctggattac tacagcgcca tgcaagtgct
    22681 ggaacgactt aaggcgctgt tttaagcatt aactgtcaga cgctgtcacc tgcggcggcg
    22741 tctggcgaaa acgtcgcaac tccaccagca ccagcaataa caacacgccg ataatcaaca
    22801 aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat catcgctccc aaaccgccca
    22861 gcagcgccgc gcctatggca tcgcccgtca cgttttgcgc cgtccacaaa ccgttaatcc
    22921 gccctaacat cgcttccggg gtttgcgttt gcagcattgt gtattgcagc aacgagctga
    22981 ccgcactcaa ccagccgaac agcgccagac aaaccacgcc taaaatccac atcggcatca
    23041 ggccaaacag accaatggcg aggaacgatc ccagcgtgga gagcagcatc aataaccctg
    23101 gtcgcgcgct atgcgccagc ttcccgctgg ttaacgcgcc aatagccgcg ccgagtggga
    23161 tcgccgcgta gagaaaacca atctgtgccg ctgacatctg ccagttgtca gccagcgccg
    23221 gatacagtac ccgcaccgcg ctcgccatcg tcaataaacc acccagcagc gcaatccctc
    23281 ctaccagcgg gctggcgagc agaaaacgaa atcctgccag taatgatttc aacggatgct
    23341 cacgcggctg cggtggcggt ggcagtgccg gaaggcttaa caacggtagc aaggtaataa
    23401 acgtgcccgc cgccgccagc ccgtagttcc aggccacacc gccagtcgcc agcagtaaac
    23461 cgccaatcat gggcgaaatc accgacccca gacgcacggt caacatggtg atcgccccgg
    23521 cctgcattaa gttttcacgc cctaccagtg ctggtgtcgc cgccaatagc gccgtaacgc
    23581 caagcgatgc gaaaaaacca tcccataaac caagtaaata gattgccagc aatgacggct
    23641 ccggcagcag cgcgttcagg cacagtccaa tgaagccaat gccacaggtg ccgcgcgcca
    23701 gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac accgccgacc atcaggccaa
    23761 caaacatcgc gccgccggtc agcgtcaccg aaagccccac ctgccaggtg gaatgtgtca
    23821 tcatctggat ctgcaccggc accgcgacgc cgagcaaacc cagagacaca atagagatga
    23881 aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt caacaggctg aggttaagca
    23941 gccaggattg tttattcatt acaatgcctt gccatcaaat attttaaata cctattctta
    24001 ggctgcacat gctaacatat ccaaataaga tcgataacga taattaattt cattatcatg
    24061 gaagttcgta tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc cggattgctg
    24121 ttattactga ttatcgcgac ggcattaagt ctgctcattg gggcaaaatc actccccgct
    24181 tccgtagtgc tggaggcctt ctccggcacc tgccagagcg ccgactgcac catcgtgctc
    24241 gacgcgcggc tgccgcgtac ccttgccggt ttactggcag gcggcgcgct tggccttgcc
    24301 ggggcgttaa tgcaaaccct cacccgaaac ccacttgccg accccggctt gcttggcgtg
    24361 aacgccggag ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta ctcttccgca
    24421 caggaacaac tggcgatggc cttcgccggg gcgctggtgg cctcattgat tgttgccttt
    24481 accggcagcc agggcggcgg gcagttaagt ccggtgcgtt taaccctggc gggcgtggcg
    24541 ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc tgacgtctac
    24601 gatcaactgc gtttctggca agccggttcg ctggatattc gcaatctaca taccttaaaa
    24661 gtggtgctga tcccggtgct gatcgccgga gcaaccgcgc tattactgag ccgcgcgctg
    24721 aacagtttga gcctcggcag tgacaccgcg acggcgctgg gtagtcgagt ggcacggacg
    24781 cagttaattg gcctgctggc gattaccgta ctttgcggta gtgcgacggc agtagttggc
    24841 ccgattgcct ttattggcct gatgatgccg catatggcgc gttggctggt gggtgccgat
    24901 catcgctggt cgctgcccgt cacgctactt gctacccctg ccctgctgct gtttgccgat
    24961 atcatcgggc gggtgattgt gcccggcgag ctgcgcgttt ctgtggtcag tgcgtttatt
    25021 ggtgcaccgg tgctgatctt cctcgtgcga cgtaaaacgc gaggtggtgc atgatttacg
    25081 tctctcgccg attactcatc acctgtttgc tgctgatttc cgcctgtgtg gttgcaggta
    25141 tctggggatt acgcagcggt gccgtcacgc tggagatctc gcaggtattc gccgcgctga
    25201 tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga atggcgttta ccacgcgtgc
    25261 tgatggcgct gttgattggc gcagcactgg gcgtcagtgg cgcgattttt cagtcgctga
    25321 tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa caccggggcg tggagcggcg
    25381 tgctggtggc gatggtgctg tttggtcagg acctgacggc tatcgcgctg gcagcaatgg
    25441 cgggcggcat tgtcacctcg ctgctggtct ggctgctcgc ctggcgtaac ggtatcgaca
    25501 cctttcggtt gattattatc ggtatcggcg ttcgcgccat gctggtggcc tttaatacct
    25561 ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg aatgccggat
    25621 cgctcaacgg cctgacgtgg gcaaaaacct cgccttccgc acccatcatt atattgatgc
    25681 tcattgccgc caccttgctg gtgcggcgaa tgcgcttgct ggaaatgggc gatgataccg
    25741 cctgcgcgct gggcgtcagc gtcgaacgtt cgcgtctgtt aatgatgctg gttgcagtgg
    25801 tgcttaccgc tgcggcaaca gcgcttgccg ggccgatttc ctttattgct ttagtcgcgc
    25861 cgcacattgc ccgacgcatt agcggcaccg ctcgctgggg gctgacccag gcggcgctgt
    25921 gcggggcgtt gttattgctg gcggccgatc tctgcgccca acaactgttt atgccgtatc
    25981 aacttccggt tggcgtcgtt accgtcagcc tcggtggtat ttaccttatc gtcttgttaa
    26041 ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgcggcg aacagttaac
    26101 cctgggatat ggcaaatata ccgttgcgga aaatctgact gtagaaatac ctgatggtca
    26161 cttcacggca attatcgggc caaatggttg cggtaaatcc acgttactgc gtaccttaag
    26221 ccgcctgatg acgcctgctc atgggcatgt ctggctggat ggcgagcaca ttcaacatta
    26281 cgccagtaaa gaagttgccc ggcggattgg tctgttggcg caaaatgcca ccacaccggg
    26341 agatatcacc gtgcaggagc tggtggcgcg tgggcgttat ccgcatcaac cgctgtttac
    26401 ccgctggcgc aaagaggatg aagaagcggt aacgaaagcg atgcaggcca cgggaataac
    26461 tcatctggca gatcaaagcg tggataccct ttctggcgga caacgccagc gagcgtggat
    26521 cgcgatggtg ctggcccagg aaacggcgat tatgctgctc gacgaaccca cgacctggct
    26581 ggatatcagt catcagattg atctactgga actgttaagc gaactgaacc gcgagaaagg
    26641 ctataccctg gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg ccagccattt
    26701 gattgcattg cgggaaggga aaattgttgc tcagggagcg ccgaaggaga ttgtcactgc
    26761 tgaactgatt gagcgcattt atggtctgcg ctgcatgatc attgacgatc cggttgccgg
    26821 aacgccactt gtggtgccgc tcggacgaac ggcaccttca atcgcaaata gttaaactaa
    26881 gtggtcagcc atcatggcat cctgttttct ggatgccatc gcatggcgca ataacacgct
    26941 accacaagcc accatcccgc cgatcagcgc ggaaaggatc acaataatcg ccttacccgg
    27001 accgtctttt ttcactggca atgacgggct taactgatat ttaaacggcg taaaattcac
    27061 atctttgata tttgcttttg ttaactgctc aacaagatac tgacgattac gtaattcacc
    27121 gttcagttcc gcaacgtcag tgaccgcttt ttcaatttcc agtttgcgtt caataccgtc
    27181 tgcgccgaga gaaatagaga aatcggggtc atctttaacg gcctgaccat tactgtaaac
    27241 gggcttttta attcctgccg cgttggcaat gtcgagtgaa taattgaggc gctgaatgtt
    27301 tgcatcaagt tgatttttca ttttaatgcg gtcctgagcc agtttttctt tttcaaactg
    27361 ggttttgatc tccagtttat tacggacgtt ttctatcgac tctttcacca ccaacgcaga
    27421 gatataatcg atatacccgc tcaaaacggt ctgcgcctct tcactggttg gcgcggtaaa
    27481 acttagcgtc caggaggtat acagtgacgg ttcatctttt ttcttactgg cattgtcatc
    27541 aaccgctttc attttttcgc tcaatgcgac aattgcgcga tgcaaatcca gttcgtcgat
    27601 tttcgcctct tttaattggt ccatcacata aggtgatgaa cgcaggtact cttccagcaa
    27661 gctaaccgac tgaaacttct tgataaacag gttaaatgct tctgtacgat caattttgat
    27721 atccagatcc agcacacgaa gcttagtgaa tgttttctcc aactcttgcc actgaacagg
    27781 ttctggaggc gtgacaaccg ccgcgctggt ccatttttgc ggcaggatga aagagatcag
    27841 caagcctgcg caggcaaacg caaaaacgac cgccatgacc gtttttttgg cccgccataa
    27901 aactgagatt agattaagta aatcaatttc attattactg ggcgacgcca gaggataatc
    27961 gggaaaatga gcgtcacttc cctgtttaat attcagtgat gacatggggt ataacctgaa
    28021 aatgagtcca atagggggaa atctccacgg cgctcacttt accagttggt gagaattatc
    28081 cgaagctgaa gtttgtaaat tcattccact gaatgcaaca ttcactctat ttaagaaaaa
    28141 taaccagcaa acttataaat taaataaatg ataaacccat aatgatttcc tgcaattatg
    28201 ggtttataga taatgttaat ttatctgttt agcgttgcgc gaataatcgg cccaattttt
    28261 tcaaatgccc ctggagagat aatatccaca tgcgcacaat cctgacgata gatatccagc
    28321 tccgcaatcc acggcgacca ggcgcgttca ggactcatac cttcctgaag cgtgcgttca
    28381 gcaacaaaca acgtcgcttt accgtcaaac ggtacgctat gagcagtcgt cagcaggcgc
    28441 acagcatcag cgtagttgcc ttcaatggtg gtaaacaact ccgttgaagt acttccctgc
    28501 tgtgctgcca ggaaggcctc gcgttcgcgg ttaatctccg ccagcacttc cgggtccaga
    28561 ccattagctt ctttttcctg ccagttttgc gtttctggcg gccaggtatc cagcaagcca
    28621 agaaatgcca cctgttcgcc acgggcacgc agtcgcgccg caataccctg cgccagcgta
    28681 ccgccaaggg aataccccag caggtaataa gggccatgcg gttgttgttc aagtaacgtt
    28741 gccagatgcg cttcacagac ttcatccaga tttgccgcgg tctgcatggg gccattgggg
    28801 cgaggcgact gaatgccgat aatcgaccat tgtggatcga gataacgcga gagcacgctg
    28861 aactgccagg caaaaccgga cgcaggatgg aaacaaaaca gcgtcgggcc attaccttca
    28921 cgcaacggca gaatggtttc gaatcccatg cgccgggcgc tgtcctcttc accatcaata
    28981 atcgttgcca gtttggcgac agttgacgcg accatcacct gccccggcgt cacctgacgg
    29041 gcaaactgcc gacttaactg cgctgccagt ttcattgcca gtagcgaatg accgccaagc
    29101 gcgaagaaat cagcatcggc atcctgcacg tcacaaccca gcaacgacgc gaatgccgca
    29161 gcgataatcg tttcactgcc cgctttcggc gcacgccccg gcgtttgtgc cttaagttca
    29221 ggcaacggta aggctttgcg atccagcttg ccgttggcgc taagaggtaa ctgtggaagt
    29281 tgcagcagaa cgaccggcac catatgcggc ggcaatgttt cgcgaagctg tgcctgtaat
    29341 gcgctggtat ccaacggcag acctgattgc gacaccagat agcccaccaa ctgacgcgca
    29401 tcaccaccgg tggctgccgc ctggttaatc acacaggcgt gggtaacggc ttgttcgaca
    29461 tccggcagcg cctgcatcac gcgatcgatt tcgcccagtt cgatacgctg cccgcgaatt
    29521 tttagctgat cgtcactgcg cccgaggtac tccaccgcgc cgttatccag ccagcgggca
    29581 acgtctccgg tacggtacat ccgttcgcca ggagcaaaag gatcggcaat aaaacggctg
    29641 gcggtcagat cgggtcgtcc aagatacccc tgcgccagtt gaatgccggt gagatagaga
    29701 tcacccgcca cacccggcgg caccggatgc atcatcgcat caagaatacg cagacccgta
    29761 ttccataccg gataaccaat cggcacactg ctgccgcgca cctgtgccag ttcctcgcca
    29821 aaagccggat accagctgac atctaccgcc gcttccgtcg ggccatatag attatgcaac
    29881 ggcacgctgg ttaactgttg ccattcgcgg cataaatcag ccggtaaggc ctcaccacta
    29941 cagaaaacct gtttcaacgt cgcgcaactc tggcgagcgg tttgcggcgt cagcgatgca
    30001 acaaatgccg ccagcatcga cggcacaaag tgcgtggtcg ttacgccata ttcggcaaag
    30061 aattgctgca tagcgagcgg gtcgcggtgc gcttccggtt cagccatcac cagttttgcc
    30121 ccggcaataa acggccagaa aaactcccac accgagacat caaaactgca cggcgttttt
    30181 tgggcaacga catcttcgcc tgtaagcggg tagtgatttt gcatccataa caggcggtta
    30241 acgatagccg tctgcccgac cattacccct ttcggtctgc cggtggaacc agaggtaaag
    30301 atgatataag ccgtgtggtg cggttgtgaa agttgcagcg gcgcactgcc ctgcggtgta
    30361 agcggggcgt tatagcaaag gttggttaaa tctggaacat cggcaaagcg cggcagttga
    30421 tcgtcggtgg tgattaacag cgacggacgc gcatcttcca gcatcatttt caggcgatcg
    30481 tccggatacc ccgtatccag cggtaaccag gccgcaccag cttcaacaat tgcatgtagc
    30541 gccagagtca aaaataccga gcgcggtagc gccaccgcca cgctgtcgcc aggtttaacg
    30601 ccgcgctcac gcagcagatt cgccagcgcc accacctgct cgcgcatttc ccgatagctg
    30661 aactggtaac gcgcatctgc cagcgccgga gcatccggtg tttttgccgc ttgttctgcc
    30721 accagcgcgc taagcgtggt ttctggaatc tcaacctgag tggcgttgat ctgcgccagc
    30781 tgcgcatatt cacctggcag cataatatcg acatcgccgc acaacagcgc cggatccgcg
    30841 gcgaactggg cgatcagcat tttcaggcgt tcagcatgct ggattaacgt tggctcatcg
    30901 taacgctgtt tattggcgag gatctcaata ctcaaatcac cgtgttcatc cgggaacagg
    30961 gccaattcaa ggtcattaac cggaccggtt gccagggtat gggtttgcgc ctgaacatca
    31021 ggaatatcca gttggtaatc aaataccttg atattgagta ccggaccaaa cagcggttca
    31081 tcacctgccg ctcgcccact gtcacggaca atttgttcgg catcgtaacg ttgatggcga
    31141 cgcatttttt tcagttgtgc tgccagtcgg gttgccagtt ccggcagcgt ttcttgcgcc
    31201 gcaatgtgaa tacccaacgg taaaacgttt agcacgggtc cggtagccgt cagcgccgcc
    31261 gagcccagtc gacgcataaa gataaatccg gcggcgtagt ccatgcgatt gcacaatcgc
    31321 cccagccaca aggctgccag cgcaagggct aaatcggtac gctgcacacc tgaaagttgc
    31381 atagccagct ggcggaattc cccgtcggta aattccagtt tcaggcgcag aatatctgcc
    31441 gaggcgctgc gccccggtaa aggtgccgga gaaagtgacg cgggcggtgg cagttggcaa
    31501 cgctgttcca cccagaatgc cgcatcacgc tgccaggctt cgctttcgcg atattgctgg
    31561 tactcttcca ctacatcagc gaaaggcgta aacggcgaag caggcgttgg ttcgccacgc
    31621 agcaatgcgc agtaaatgtt ggcgatctgg cgggtaatgg ccggaaaact gaagccatcg
    31681 accagtaaat ggtgataacg ctgataccag taccagcggt tatctgccac ctgtatcagc
    31741 tggtgaaaga ccagtggttt accgctatcg acgcgcaaat cttgttgcaa atccgcctgc
    31801 attaatgcct gcgcagtacc gtgcggatca atgttggttc gcaggtcgat aatttctggc
    31861 agttcgaata tcagcgcatc atcgacccat tgccagactt cgccgttatc ttccgtaaaa
    31921 cgcatccgca acgtatctgc ttgcgctaat ccggcaacca ccgcgcgggc cagtaatggc
    31981 gcatcaacct ctccggttaa ctcaacgtaa tgcgccacgc tccaggcgga gggtaaatcc
    32041 gacagttttt ctgccatcca gatgccgggc tgtgcggcga ccaaaggtaa atgctggctc
    32101 attgtgcctc ctgcaactgg gtgaaattcg ccggtgtcag agtacgccag tgggcttcaa
    32161 gccactgctg gcaggactct tgtgactgcg gctgacacac aatgtcccag cctgccggta
    32221 aggcgcattg ttgcggccac agactgaatt gcccctgcgc attgcgcaaa atgtaaaaca
    32281 ctccctgtgg atcatcgaag ggattactga atgccatatt caactcctgt catggaaaag
    32341 tggttgccag aggtcgatta acccctgcat caagccaccg cgccaacaaa gcgcatcatg
    32401 tccgccgtca acctgacgcc agaaaatgga ttcttttata gggtgtaatt gcgcatacag
    32461 cgcctgattg gcccgcatga tcatcggctc gcgaataccc gcttccagca caatgcgcag
    32521 accttcggcg ctaacttcac cagcttctag cttttcaagt aacacgccct cttgctgccc
    32581 gccccgatgc ggccaccagt acgatcctga ctggcttaat acacagccaa agcgttcagg
    32641 ccagtgcagt ccggcataca gcgcggacag cccaccaaaa ctctgcccgg cgaccacggt
    32701 gcgatcggcg cgatcgctaa aaggggcaat agctttcacc aggggcaata actcttgctg
    32761 tactgccagc cagaaatccg cattacacgg cagttcgtgg gcgcggtgcg tggtgtcgat
    32821 agcgtcgatc aacacataca cggcgggagg aagttgctga cgatgggtca gcgaagtcag
    32881 cacaggccag acgggcatac tttgcgccca aaattcgcca tcgagcaaaa ctgccagcgg
    32941 gcgttcttct gctgttacat cgccggtggt aaaaatccat acacgacgtg aatttttcaa
    33001 ccgttcactt ttccagataa tttctttggc tggtatttct ggcgcttgcg gacaatccca
    33061 tcccggttgc agaggcgctt gcggcatttc gagtgcagaa acagcgtgcc ctcgcccgcc
    33121 tttccagctt tgtggattca gcggatcggc tattgcctgg ggtaatagtt ttcgccagcc
    33181 ttcacgtaat tcgaggcgat cgggggatgg tgcagaaaaa atgtcatcgc gttcggtggg
    33241 aataaagcag tagctgccgc gccagttggc attgagttgt gtcgtccact gccagacatc
    33301 agtacctgca attcgctgca tcgactgggg ctggctgttc tgatgatgat cggtcacacc
    33361 agtgatgtag acccatacgc gctttatcgt cgagtattct tcagaacctt ggggatcacg
    33421 ccaccagaaa gtgacctcaa acatttcgtc attcagacgc tgccattccg ggccatgttt
    33481 cgactgccac cagctctcac ttcctacttt taacgccgtc acaccataac cccatgttta
    33541 ttgtgcaatt tt
//
LOCUS       DAZFFG010000040        33406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFFG010000040 DAZFFG010000000
VERSION     DAZFFG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.040"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            100..1389
                     /gene="hpaG"
                     /locus_tag="ACHJ7J_002263"
     CDS             100..1389
                     /gene="hpaG"
                     /locus_tag="ACHJ7J_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO5000495.1"
                     /translation="MKGTIFAVALNHRSQLDAWQDAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQSEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1386..2852
                     /gene="hpaE"
                     /locus_tag="ACHJ7J_002264"
     CDS             1386..2852
                     /gene="hpaE"
                     /locus_tag="ACHJ7J_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5000496.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2854..3705
                     /gene="hpaD"
                     /locus_tag="ACHJ7J_002265"
     CDS             2854..3705
                     /gene="hpaD"
                     /locus_tag="ACHJ7J_002265"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO5000497.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3715..4095
                     /locus_tag="ACHJ7J_002266"
     CDS             3715..4095
                     /locus_tag="ACHJ7J_002266"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO5000498.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4163..4966
                     /gene="hpaH"
                     /locus_tag="ACHJ7J_002267"
     CDS             4163..4966
                     /gene="hpaH"
                     /locus_tag="ACHJ7J_002267"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO5000499.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4977..5765
                     /gene="hpaI"
                     /locus_tag="ACHJ7J_002268"
     CDS             4977..5765
                     /gene="hpaI"
                     /locus_tag="ACHJ7J_002268"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO5000500.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPGVY"
     gene            5941..7317
                     /gene="hpaX"
                     /locus_tag="ACHJ7J_002269"
     CDS             5941..7317
                     /gene="hpaX"
                     /locus_tag="ACHJ7J_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO5000501.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7327..8217
                     /gene="hpaA"
                     /locus_tag="ACHJ7J_002270"
     CDS             7327..8217
                     /gene="hpaA"
                     /locus_tag="ACHJ7J_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO5000502.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8468..10030
                     /gene="hpaB"
                     /locus_tag="ACHJ7J_002271"
     CDS             8468..10030
                     /gene="hpaB"
                     /locus_tag="ACHJ7J_002271"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO5000503.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSPDDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHAQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10048..10560
                     /locus_tag="ACHJ7J_002272"
     CDS             10048..10560
                     /locus_tag="ACHJ7J_002272"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO5000504.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10953..13103
                     /gene="btsT"
                     /locus_tag="ACHJ7J_002273"
     CDS             10953..13103
                     /gene="btsT"
                     /locus_tag="ACHJ7J_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO5000505.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13153..13356
                     /locus_tag="ACHJ7J_002274"
     CDS             13153..13356
                     /locus_tag="ACHJ7J_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5000506.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13367..14323
                     /gene="yjiA"
                     /locus_tag="ACHJ7J_002275"
     CDS             13367..14323
                     /gene="yjiA"
                     /locus_tag="ACHJ7J_002275"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5000507.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14369..15283)
                     /gene="mrr"
                     /locus_tag="ACHJ7J_002276"
     CDS             complement(14369..15283)
                     /gene="mrr"
                     /locus_tag="ACHJ7J_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HFO5000508.1"
                     /translation="MTVPTYDKFIEPVLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     AKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQET
                     NHLAFDFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            15471..18983
                     /gene="hsdR"
                     /locus_tag="ACHJ7J_002277"
                     /pseudo
     CDS             15471..18983
                     /gene="hsdR"
                     /locus_tag="ACHJ7J_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418770.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
     gene            19060..20649
                     /gene="hsdM"
                     /locus_tag="ACHJ7J_002278"
     CDS             19060..20649
                     /gene="hsdM"
                     /locus_tag="ACHJ7J_002278"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     methyltransferase"
                     /protein_id="HFO5000509.1"
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETG
                     QEAEYLPEGYRWDDLKSRIGQEQLQFYRNLLVHLGADNQKLVQAVFQNVNTTITQPKQ
                     LTELVSNMDSLDWYNGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQHLATSRWRKF
                     SREWIRTAKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALGELDALMRELG
                     ASDEADAQRQLLEEAFGGVKE"
     gene            20646..22055
                     /locus_tag="ACHJ7J_002279"
     CDS             20646..22055
                     /locus_tag="ACHJ7J_002279"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5000510.1"
                     /translation="MSEKTRPESWLSKIAIDCFDQISTTNNKVKSTACSPHGLYPVID
                     QGQNYISGYINDESKLIKITTPICIFGDHTRIIKWVEHNFVPGADGTKLLKPKDFIYP
                     RYAYYSLCALNIPDKGYSRHFKYFKELKIDIPAWEEQKIIAEKLDTLLAQVESTKARL
                     EQIPQILKRFRQAVLGTAMSGNLTGEWRLSNHSIILAEEIEENKNRLIEKKLVKKDLT
                     YASLDKRYSLPPSWLYIKLQSVSTKITDGKHKTPKRETAGKFLLSARNIQDGYIKLAD
                     VDYVGDAEFQKLRNRCDPDYGDILISCSGSIGRVCLVDENSKYVMVRSVALVKLMQDF
                     VINKYMMFLLQSPLLQKEIEENSKSTAQSNLFLGPIKNLGIPLPPVSEQHEIVRRVEQ
                     LFAYADTIEKQVNNALARVNNLTQSILAKAFRGELTAQWRAENPELISGENSAAALLE
                     KIKAERAASGGKKASRKKS"
     gene            22283..22624
                     /gene="symE"
                     /locus_tag="ACHJ7J_002280"
     CDS             22283..22624
                     /gene="symE"
                     /locus_tag="ACHJ7J_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO5000511.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEVAGFATGTVVDVKVMEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQKQVQAF
                     IGVIAGKQKVA"
     gene            complement(22671..23834)
                     /locus_tag="ACHJ7J_002281"
     CDS             complement(22671..23834)
                     /locus_tag="ACHJ7J_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO5000512.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGVLSH
                     EDFLSIIRLIESYVFRRAVYAIPTNSLNKTFATFYKVINKENYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GLHSRLLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(23882..24764)
                     /locus_tag="ACHJ7J_002282"
                     /pseudo
     CDS             complement(23882..24764)
                     /locus_tag="ACHJ7J_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(24861..25025)
                     /locus_tag="ACHJ7J_002283"
     CDS             complement(24861..25025)
                     /locus_tag="ACHJ7J_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO5000513.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            25202..26614
                     /locus_tag="ACHJ7J_002284"
     CDS             25202..26614
                     /locus_tag="ACHJ7J_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5000514.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPSDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHNIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAIE"
     gene            complement(26856..27799)
                     /locus_tag="ACHJ7J_002285"
                     /pseudo
     CDS             complement(26856..27799)
                     /locus_tag="ACHJ7J_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            28266..29498
                     /gene="mdtM"
                     /locus_tag="ACHJ7J_002286"
     CDS             28266..29498
                     /gene="mdtM"
                     /locus_tag="ACHJ7J_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO5000515.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGRTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSMLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            29539..30819
                     /locus_tag="ACHJ7J_002287"
     CDS             29539..30819
                     /locus_tag="ACHJ7J_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO5000516.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADIDSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            30935..32086
                     /locus_tag="ACHJ7J_002288"
     CDS             30935..32086
                     /locus_tag="ACHJ7J_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO5000517.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKELPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            32096..32863
                     /gene="yjiL"
                     /locus_tag="ACHJ7J_002289"
     CDS             32096..32863
                     /gene="yjiL"
                     /locus_tag="ACHJ7J_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO5000518.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGAIGAAVIGQRARKRR"
     gene            32860..33126
                     /locus_tag="ACHJ7J_002290"
     CDS             32860..33126
                     /locus_tag="ACHJ7J_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO5000519.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
BASE COUNT         8104 a         8787 c         8719 g         7796 t
ORIGIN      
        1 tcacatttac atcacttact tttgccaaac tacgcaatcg ggaataaaag taatcattaa
       61 catattaatg attaagatga tcgaatccga ggagtggtaa tgaaaggcac tatcttcgcc
      121 gtagcgttga accatcgcag ccagcttgat gcatggcagg acgcgttcca gcaatccccc
      181 tacaaagccc cgcctaaaac tgcggtctgg tttattaaac cgcgcaatac ggtgattggt
      241 tgcggtgaac cgattccctt tccacagagt gaaaaggtac tgagcggtgc gacagttgcg
      301 ctgattgtgg gaaaaacggc gacgaaagta cgtgaagaag atgcggcaga gtacatcgcc
      361 ggatatgcgc tggctaacga cgtcagcctg ccggaagaga gcttttaccg tccggcaatc
      421 aaagcaaaat gtcgtgatgg attctgccca attggcgaaa ccgtggctct cagcaatgtc
      481 gataatctga ctatctatac cgagatcaac gggcgtcctg ccgatcactg gaacaccgcc
      541 gatttacaac gtaacgccgc acagctgctg agcgccctga gcgaatttgc cacgctgaac
      601 ccaggcgatg ccattctgct cggcacgcca caggcgcgcg tggaaataca gccaggcgat
      661 cgcgtgcgtg ttctcgcaga aggtttcccg ccgctggaaa atccggtagt ggacgaacgt
      721 gaagtgacca cgcgcaagag cttcccaacg ctgccacacc cgcacggtac gctgtttgcc
      781 ctcggcctga actacgccga ccacgccagc gaactggaat ttaagccacc ggaagaaccg
      841 ctggtgttcc tgaaagcgcc gaataccctc actggcgata accagacctc cgtgcgcccg
      901 aacaatattg aatacatgca ctacgaagcg gagctggtgg tggtgattgg caagcaggcg
      961 cgtaacgtca gcgaagccga tgccatggat tatgtcgcgg gctacaccgt gtgtaacgac
     1021 tacgccattc gcgactacct ggaaaactac taccgcccta acctgcgggt aaaaagccgc
     1081 gacggactga cgccgatgct ttcaaccatc gtgccgaaag aggcgatccc ggacccgcat
     1141 aatctgaccc ttcgcacctt cgtcaacggc gagttacgcc agcaaggcac caccgccgat
     1201 ctgatcttca gcgtgccctt cctgatcgcc tacttaagcg aatttatgac cctgaatccg
     1261 ggcgacatga tcgccaccgg cacaccaaaa ggcttatctg acgtggtgcc tggcgatgaa
     1321 gtggtggtgg aagtagaagg cgtgggccgc ctggtgaacc gaattgtgag tgaggataca
     1381 gcgaaatgaa aaaagtaaat cattggatca acggcaaaaa tgttgcaggt aacgactact
     1441 tccagaccac taatccggca acgggtgaag tgctggcgga tgtggcctct ggcggtgaag
     1501 cggagatcaa tcaggcagta gcggcagcga aagaggcgtt cccgaaatgg gccaatctgc
     1561 cgatgaaaga gcgtgcgcgc ctgatgcgcc gtctgggcga tctgatcgac cagaacgtgc
     1621 ctgagatcgc cgcgatggaa accgcggaca cgggcctgcc gatccatcag accaaaaatg
     1681 tgttgatccc acgcgcttct cacaactttg aatttttcgc ggaagtctgc cagcagatga
     1741 acggcaagac ctatccggtt gacgacaaga tgctcaacta cacgctggtg caaccggtag
     1801 gcgtttgtgc gctggtatcg ccgtggaacg tgccgtttat gaccgccaca tggaaggtcg
     1861 cgccgtgtct tgcgctgggc aataccgcgg tgctgaagat gtcagaactc tccccgctga
     1921 ccgctgaccg cctgggtgag ctggcgctgg aagccggtat tccggcgggc gtgctgaacg
     1981 tggtacaggg ctacggcgca accgcagggg atgcgctggt ccgtcatcat gacgtgcgtg
     2041 ccgtgtcgtt caccggcggt accgccaccg ggcgcaacat catgaaaaac gccgggctga
     2101 aaaaatactc catggaactg ggcggaaaat cgccagtgct gatttttgaa gatgccgata
     2161 ttgaacgcgc gctggacgcc gccctgttca ccatcttctc gatcaacggc gagcgctgca
     2221 ccgccggttc gcgcatcttt attcagcaaa gcatctaccc ggaattcgtt aaacgctttg
     2281 ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga tccgaatacc caggttgggg
     2341 cacttatcag ccagcaacac tgggaaaaag tctccggcta tatccgtctc ggcattgaag
     2401 aaggcgcaac cctgctggcg ggcggcccgg ataaaccgtc tgacctgcct gcgcacctga
     2461 aaggcggcaa cttcctgcgc ccaacggtgc tggcagacgt agataaccgt atgcgcgtcg
     2521 cccaggaaga gattttcggg ccggtcgcct gcctgctgcc gtttaaagac gaagccgaag
     2581 gcttacgcct ggcaaacgac gtggagtacg gcctcgcgtc gtacatctgg acacaggatg
     2641 tcagcaaagt gttacgcctg gcgcgtggca ttgaagcagg catggtgttc gtcaacaccc
     2701 agaacgtccg cgacctgcgc cagccgtttg gcggcgtaaa agcctccggc accgggcgtg
     2761 aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa gaacgtctgc atttccatgg
     2821 gcgaccatcc aattccgaaa tggggagtct gatatgggta agttagcgtt agccgccaag
     2881 atcacgcacg taccgtcgat gtatctctct gaactaccag gaaaaaacca cggttgtcgc
     2941 cagggtgcga tcgacgggca taaagagatc agcaagcgtt gccgggaaat gggcgtcgat
     3001 accattatcg ttttcgacac ccactggctg gtcaacagtg cttatcacat caactgtgca
     3061 gaccattttg aaggcgtcta caccagtaac gaactgccgc attttattcg tgacatgacc
     3121 tacaactacg agggcaaccc ggagttgggg cagctaattg ccgatgaagc cttaaagctc
     3181 ggcgtgcggg caaaagcgca caatattccc agcctgaaac tggaatacgg cacgctggtg
     3241 ccgatgcgct acatgaatga agacaagcac ttcaaagtgg tctccatttc ggctttctgc
     3301 acggttcacg attttgccga cagccgcaag ctgggcgaag cgattctgaa agcgatcgaa
     3361 cagtacgacg gcaccgtggc ggtccttgcc agcggttcgt tatcgcaccg ctttattgac
     3421 gatcagcgtg cggaagaagg gatgaacagc tacacccgcg agttcgaccg ccagatggac
     3481 gagcgcgtgg tgaagctgtg gcgcgaaggt cagttcaaag agttctgcaa tatgctgccg
     3541 gagtacgccg actactgcta cggcgaaggc aatatgcacg acacggtgat gctgttgggg
     3601 atgctcggct gggataaata cgacggcaag gtggagttta ttaccgagct gttcccaagc
     3661 tctggcaccg gtcaggttaa cgctgttttc ccgctgcccg cgtaaggacg ttttatgccg
     3721 cactttatcg tggaatgtac tgaaaatatt cgtgaagaag cccgcttacc tgagctgttt
     3781 gccagtgtta acactgcact ggcggcaacg gggatttttc cgcttggggg aatacgtagc
     3841 cgggcgcact ggatcgatac ctggcaaatg gctgatggca agcacgatta cgcctttgtg
     3901 catatgacgc taaaaatcgg cagcggacgc agtctggaaa gccgtcagga agtgggcgaa
     3961 atgctgtttg atctgattaa aacgcacttc gcctcactga tggagagccg ttacctggcg
     4021 ctttcatttg agattgcaga gctgcacccg acgttgaatt tcaaacagaa caacgtacac
     4081 gcgttgttta aataaatttg caccagcttc caggccggat gataaggcgc tgccgcatcc
     4141 ggcaatgcca caggatatcg ctatgttcga taaacacacc cacaccctga tcgcccagcg
     4201 tctggatcag gcagaaaaac agcgcgaaca gatccgcgcg atctcgctgg attacccgga
     4261 gatcaccatc gaagacgcct acgccgtaca gcgcgaatgg gtccgcctga aaattgccga
     4321 aggccgtacg ctgaaaggcc acaaaatcgg cctgacctcg aaagcgatgc aggccagctc
     4381 gcagatcagc gaaccagatt acggtgcact gctggacgac atgttcttcc acgatggcag
     4441 cgatatcccg accgatcgct ttatcgtgcc gcgcattgaa gtggagctgg cttttgtgct
     4501 ggcaaaaccg ctgcgtggac caaactgcac gcttttcgac gtttacaacg ccacggacta
     4561 tgtgatcccg gcgctggagc tgatcgacgc ccgctgccac aacatcgatc ctgaaaccca
     4621 gcgcccgcgc aaagtattcg acaccatttc cgataacgcc gccaacgccg gagtgatcct
     4681 cggcggtcgt ccgatcaagc cagacgaact ggatctgcgt tggatctccg ccctgatgta
     4741 tcgcaatggc gtgattgaag aaaccggcgt cgccgctggt gtgctgaatc atccggcaaa
     4801 cggcgtggcc tggctggcga acaaactcgc accgtatgac gtacaactgg aagccgggca
     4861 aatcattctc ggcggttcgt ttacccgccc ggttccggcg cgtaagggcg acaccttcca
     4921 cgtcgattac ggcaacatgg gttccattag ctgccgcttt gtttaaggag agaacgatgg
     4981 aaaacagttt taaagcggcg ctgaaagcag gccgcccgca gatcggatta tggctggggc
     5041 tgagtagcag ttacagcgca gagttactgg ccggagcagg attcgactgg ttgttgatcg
     5101 acggtgagca cgcaccgaac aacgtacaaa ccgtgctcac ccagctacag gcgattgcgc
     5161 cctatcccag ccagccggtg gtacgcccat cgtggaacga tccggtgcaa atcaaacaac
     5221 tgctggatgt cggcacacaa accttactgg tgccgatggt tcaaaacgct gacgaagccc
     5281 gtgaagcggt acgcgccacc cgttatcccc ccgccggtat tcgcggtgtg ggcagtgcgc
     5341 tggcccgagc ctcgcgctgg aatcgtattc ctgattacct gcaaaaagcc aacgatcaaa
     5401 tgtgcgtgct ggtgcagatc gaaacgcgtg aggcaatgaa gaacttaccg cagattctgg
     5461 acgtggaagg ggtcgacggc gtgtttatcg gcccggcgga tctgagcgcc gatatgggtt
     5521 atgccggtaa tccgcagcac ccggaagtac aggccgccat tgagcaggcg atcgtgcaga
     5581 tccgcgaagc gggcaaagcg ccggggatcc tgatcgccaa tgagcaactg gcaaaacgct
     5641 atctggaact gggcgcgctg tttgtcgccg tcggcgttga caccaccctg ctcgcccgcg
     5701 ccgccgaagc gctggcagca cggtttggcg cgcaggctac agcgattaag cccggcgtgt
     5761 attaatgccg gatgcgctgc gcttatccgg cctacactcg caccaaaccg taggccggat
     5821 aaggcgttta cgccgcatcc ggcaaaaagc cgtaccaaat cgcggaccgg ataaggcgtt
     5881 tacgccgcat ccgccataaa ccttgtcgta ccctacaaaa atcccattag aggaagaaaa
     5941 atgagcgaca cctcacctgc cataccggag agtatcgatc cggcgaatca gcataaagcg
     6001 ctgactgccg gacaacaggc ggttattaag aagctatttc gccgcctgat cgtctttctg
     6061 ttcgtgctgt ttatcttctc gttccttgat cgcatcaaca tcggctttgc cggactcacg
     6121 atgggacgcg acctcggtct gagcgccacc atgtttggcc tcgctaccac cctgttctat
     6181 gccgcttatg tcatcttcgg cattcccagc aacattatgc tgagtattgt cggtgcgcgg
     6241 cgctggatcg ccaccatcat ggtgctttgg ggcatcgcct ctactgccac catgtttgcc
     6301 actggcccca ccagcttata cgtactgcgt atactggttg gcattaccga agccggcttt
     6361 ctgcctggca ttctgctcta tttaaccttc tggtttccgg cctacttccg cgcccgtgcc
     6421 aacgccttgt ttatggtggc aatgccggta acgacagcgt tgggatcgat cgtttccggc
     6481 tacattttgt cgctggatgg cgtaatggca ttaaaaggct ggcagtggct gtttttgctg
     6541 gaaggcttcc cgtcggtatt actcggcgtc atggtgtggt tctggcttga tgactcaccg
     6601 gacaaagcta agtggctgac gaaagaagac aaaaaatgcc tgcaagagat gatggataac
     6661 gatcgtctga cgctggttca gccagaggga gccatcagcc accacgccat gcaacaacgc
     6721 agcatgtggc gggagatctt cactccggtg gtgatgatgt ataccctggc gtatttctgc
     6781 ctgaccaaca cacttagtgc gatcagcatc tggacaccgc agatcctgca aagcttcaat
     6841 cagggcagca gtaatatcac catcggcctg ctggccgccg taccgcagat ttgtaccatt
     6901 ctcgggatgg tctactggag ccgtcattca gatcgccgcc aggaacgaag gcatcacacc
     6961 gcccttcctt atttgttcgc tgccgctggt tggttactgg cttcggcaac tgatcacaac
     7021 atgatccaga tgctggggat cattatggct tcgaccggat cattcagcgc aatggcgatt
     7081 ttctggacaa caccggatca gtccatcagc ctgcgggcac gagcgatcgg tattgcggtg
     7141 atcaacgcca ctggcaacat tggttcagcg ttaagtccgt ttatgatcgg ctggttgaaa
     7201 gatctgaccg gcagctttaa cagtggattg tggtttgttg ccgcgctgct ggtgattggt
     7261 gcggggatta tctgggcgat tccaatgcag tcctcccgtc cgcgagcgac cccgtaagga
     7321 acgacgatgt gtgaccgtca gattgccaat attgatatca gcaaagagta cgatgaaagc
     7381 ctgggcacag acgatgtgca ttatcagtcg ttcgcccgca tggcggcatt ttttggccgc
     7441 catatgctgc cacatcgcca cgaacagtac tttcagatgc atttcctcaa tagcggacag
     7501 attgagctac agcttgacga tcatcgctac tcggtggaag cgcccctgtt tgtcctgacg
     7561 ccgccgtcag tacctcatgc gtttattacg gagtctgacg ccgacggtca tgtgttgacg
     7621 gtacgggaag atctgatctg gcccctgctg gaagttcttt atccgggcac tcgggaaacc
     7681 ttcggcctgc cggggatttg cctgtcactg gcagataaac ccgacgaact ggcggcgctg
     7741 gaacactatt ggcaactgat agagcgggaa tcggtagaac aactgcctgg tcgggaacac
     7801 accctgacgt tactggcaca ggcagtgttc accctactgc tgcgtaacgc aaaactcgac
     7861 gaccatgccg ccagcggaat gcgcggagaa ttaaaactgt tccagcgttt taatatgctg
     7921 attgaaagcc attttcatca gcactggaca gtaccggatt acgctaacga actgcatatc
     7981 accgaatcac gcctcacgga catctgccgc cgctttgcca accgtccgcc aaaacggttg
     8041 attttcgaca ggcagctgcg agaagccaag cggcttctgc tgttttctga taacgccgtg
     8101 aacaatattg cctggcaact cggttttaag gatccggctt attttgcgcg cttttttaat
     8161 cgtttagtcg gttgctcgcc cagtgcttat cgtgccaaaa aagtacctgt gacgtgaaaa
     8221 tcccttagcc ttaacgggaa accaggcacc acctgctatt cccctttctc cgcctggagc
     8281 aaggggattt agtaacgtta aaaatcgcta aaagcgacct cgatcacaaa accttacccg
     8341 acctgaaaag taccagccat tgtcccaaaa gtgtcttccc gtaatcgccc ctgcaagctt
     8401 caattgaata acaaaaacaa cacacaaaat taacaatcgt attccgatta atactgtaga
     8461 ggtcgatatg aaaccagaag atttccgcgc cagtacccaa cgtccgttca ccggggaaga
     8521 gtatctgaaa agcctgcagg atggtcgcga gatctatatc tatggcgagc gagtgaaaga
     8581 cgtcactact catccggcat ttcgtaatgc ggcagcgtct gttgcccaac tgtacgacgc
     8641 gctgcacaaa ccggagatgc aggactctct gtgctggaac accgacaccg gcagtggcgg
     8701 ctatacccat aaattcttcc gcgtggcgaa aagcccggac gacctgcgcc agcaacgcga
     8761 cgccatcgct gagtggtcac gcctgagcta tggctggatg ggccgtaccc cagactacaa
     8821 agccgctttc ggttgcgcac tgggcgcgaa tccgggcttt tacggtcagt tcgagcagaa
     8881 cgcccgtaac tggtataccc gtattcagga aactggcctc tactttaacc acgcgattgt
     8941 taacccaccg atcgatcgtc atttgccgac cgataaagtg aaagacgttt acatcaagct
     9001 ggaaaaagag actgacgccg ggattatcgt cagcggtgcg aaagtggttg ccaccaactc
     9061 ggcgctgact cactacaaca tgattggctt cggctcggca caagtgatgg gcgaaaaccc
     9121 ggacttcgca ctgatgttcg ttgcgccaat ggatgccgat ggcgtgaaat taatctcccg
     9181 cgcctcttat gagatggtcg cgggtgctac cggctcgcca tacgactacc cgctctccag
     9241 ccgcttcgat gagaacgatg cgattctggt gatggataac gtactgatcc catgggaaaa
     9301 cgtgctgatc taccgcgatt ttgatcgctg ccgtcgctgg acgatggaag gcggttttgc
     9361 ccgtatgtat ccgctgcaag cctgtgtgcg cctggcagtg aaattagact tcattacggc
     9421 actgctgaaa aaatcactcg aatgtaccgg caccctggag ttccgtggtg tgcaggccga
     9481 tctcggtgaa gtggtggcgt ggcgcaacac cttctgggca ttgagtgact cgatgtgttc
     9541 tgaagcgacg ccgtgggtca acggggctta tttaccggat catgccgcac tgcaaaccta
     9601 tcgcgtattg gcaccaatgg cctacgcgaa gatcaaaaac attatcgaac gcaacgttac
     9661 cagtggcctg atctatctcc cctccagtgc ccgtgacctg aacaatccgc agatcgacca
     9721 gtatctggcg aagtatgtgc gcggttcgaa tggtatggat cacgcccagc gcatcaagat
     9781 cctcaaactg atgtgggacg ccattggcag cgaatttggt ggtcgtcacg agctgtatga
     9841 aatcaactac tccggtagcc aggatgagat tcgcctgcaa tgtctgcgcc aggcacaaag
     9901 ctccggcaat atggacaaga tgatggcgat ggttgatcgc tgcctgtcgg aatacgacca
     9961 gaacggctgg actgtgccgc acctgcacaa caacgacgat atcaacatgc tggataagct
    10021 gctgaaataa cgcagcagga ggttaagatg caattagatg aacaacgcct gcgctttcgt
    10081 gacgcgatgg ccagcctgtc ggcagcggta aatattatca ccaccgaggg cgacgccgga
    10141 caatgcggga ttacggcaac ggccgtctgc tcggtcactg atacaccacc atcgctgatg
    10201 gtgtgcatta acgccaacag tgcgatgaac ccggtttttc agggcaacgg caagttgtgc
    10261 gtcaacgtcc tcaaccatga gcaggaactg atggcacgcc acttcgcggg catgacaggc
    10321 atggcgatgg aagagcgttt tagcctctca tgctggcaaa aaggtccgct ggcgcagccg
    10381 gtgctaaaag gttcgctggc cagtcttgaa ggtgagatcc gcgatgtgca ggcaattggc
    10441 acacatctgg tgtatctggt ggagattaaa aacatcatcc tcagtgcaga aggtcacgga
    10501 cttatctact ttaaacgccg tttccatccg gtgatgctgg aaatggaagc tgcgatttaa
    10561 gctccagccc cttcacgcat tcgtcgaagg ggcattttta ttgtgatctc aatcacatat
    10621 ccacccgatg aataattctt gcccaaccgc tcaccgccat tcctgaccgc ttacatccct
    10681 aaaataacca ctcagttatt taccttactt tacgcgcgcg tatctctggc aacatcactg
    10741 caggatagcg gtcaatttac ctcctcaaac gcaacgcaaa cctagaacgg cttcggccta
    10801 tgattaacaa attactctat ggattttggt ttgcaggaag gcagcaagtg agtgaatccc
    10861 cgggagctta caaaagtaag tgtcagaggt gaacgaacgc agctgccgca cctgtaagcc
    10921 aaaagacgac gagtaaaatg ccaggtttta ctatggatac gaaaaaacta ttcaagcaca
    10981 taccctgggt gattctcgga atcatcggtg cattctgcct cgcggtagtt gcattacgtc
    11041 ggggggagca cgtcagcgcc ctgtggatcg tggtcgcctc tgtgtcggtg tatctggtgg
    11101 cgtatcgcta ctacagtttg tatatcgccc agaaggtgat gaaactcgac cccacgcgcg
    11161 cgacgcctgc ggttattaac aacgacggtc tgaactacgt tccgaccaac cgttacgtgt
    11221 tgtttggtca ccacttcgcc gctatcgccg gtgctggtcc gctggttggt ccggttctcg
    11281 ccgcgcagat gggctacctg cctggcacac tgtggctgct ggcgggggta gtgctggccg
    11341 gtgcggttca ggactttatg gtgctgttta tctcctctcg ccgtaacggc gcatctctcg
    11401 gtgagatgat caaagaagag atgggaccag taccggggac tatcgcgctg tttggctgtt
    11461 tcttaatcat gatcatcatc cttgccgtcc tggcgctgat tgtggttaaa gccctggccg
    11521 aaagtccgtg gggtgtcttc accgtttgct caaccgtacc aattgcgctg tttatgggta
    11581 tctacatgcg ctttatccgt ccggggcgtg tgggtgaagt ctctgtcatt ggtatcgtgc
    11641 tgctggttgc ctctatctac ttcggtggcg tgattgctca cgatccgtac tggggtccgg
    11701 cactgacctt taaagacacc accattacct tcgcgctgat tggctatgcg tttgtttccg
    11761 cgctgctgcc ggtgtggctg atcctcgcac cacgcgacta tctggcaacc ttcctgaaaa
    11821 tcggcgttat cgtcggcctg gcgctgggta tcgtggtgct gaacccggaa ctgaaaatgc
    11881 ctgcgatgac ccagtacatt gacggtactg gcccgctgtg gaaaggcgct ctgttcccgt
    11941 tcctgttcat caccatcgcc tgtggtgcgg tatctggctt ccacgcactg atctcttccg
    12001 gtactacgcc gaaactgctg gctaatgaaa ccgacgcgcg tttcatcggt tatggcgcaa
    12061 tgctgatgga gtccttcgtg gcgattatgg cgctggttgc agcgtccatc atcgaaccgg
    12121 gtctttactt cgcgatgaac accccgcctg ctggccttgg catcaccatg cctaacctgc
    12181 atgaaatggg tggcgagaac gcgccgatca tcatggcgca gttgaaagac gttaccgcac
    12241 acgcggcggc gaccgtcagc tcctggggct tcgtgatttc gccagagcag atcctgcaaa
    12301 ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc aggtggtgcg ccgacgctgg
    12361 cggtaggtat cgctcatgtg ttccacaaag tgctgccgat ggctgacatg ggcttctggt
    12421 atcacttcgg tattctgttc gaagccctgt tcatcctgac cgcgctggat gcgggtaccc
    12481 gttctggccg ctttatgctg caagatctgc tgggtaactt catcccgttc ctgaaaaaaa
    12541 ccgactctct ggttgccggt atcatcggta ctgcgggctg tgtgggtctg tggggctacc
    12601 tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa gagcctgtgg ccgctgttcg
    12661 gtatctccaa ccagatgctg gcagccgtag cgctggtact gggcaccgtt gtgctgatta
    12721 agatgaagcg cacccaatac atctgggtaa ctgttgttcc ggctgtatgg ctgcttatct
    12781 gcaccacctg ggcgctgggc ctgaaactgt tcagcaccaa cccgcagatg gaaggcttct
    12841 tctacatggc aagccagtac aaagagaaga ttgctaacgg tactgacctg acggcgcagc
    12901 agattgccaa catgaaccac atcgttgtga acaactacac caacgcaggc ctgagtattc
    12961 tgttcctgat tgtggtgtac agcatcatct tctacggttt caaaacctgg ctgaaggtgc
    13021 gtaacagcga caaacgtact gacaaagaaa ctccgtacgt tccaatcccg gaaggcggcg
    13081 tgaagatttc ttcgcaccac taaccgtgtt tagccccgct tcggcggggc tttgttctta
    13141 tcagagtgaa ctatgtttgg taacttagga caggcaaaaa aatatctcgg ccaggcggcg
    13201 aagatgttga ttggcattcc agactacgac aactacgtcg agcatatgaa gaccaaccat
    13261 cccgacaagc cgtacatgag ctatgaagaa ttcttccgcg aacgccagaa cgcgcgctac
    13321 ggcggcgatg gtaaaggcgg tatgcgctgt tgttaatgga gagagcatga acccgattgc
    13381 agttacccta ctcaccggtt ttttaggcgc gggaaaaacc accctgctgc gccatattct
    13441 taacgaacaa cacggctaca agattgccgt gattgaaaac gaattcggcg aagtctccgt
    13501 tgatgatcaa ctaattggcg accgcgccac gcagatcaaa acgctgacca acggctgcat
    13561 ctgctgttcg cgctccaacg agctggaaga cgcactactc gacctgctgg ataatctcga
    13621 taagggcaat attcagttcg atcgtctggt cattgaatgc accggcatgg ccgatcccgg
    13681 cccgattatt cagacctttt tctcccatga gattttatgc cagcgttacc tgctggacgg
    13741 cgtgattgcg ctggtggatg cggtacatgc cgatgagcag atgaaccagt tcaccatcgc
    13801 ccagtcccag gttggctacg ccgaccgtat tctgctgact aaaaccgacg tcgcaggcga
    13861 agcagaaaaa ctgcgtgaac gcctggcgcg catcaacgcc cgtgcaccgg tctacaccgt
    13921 cacccacggc gacatcgacc tgggcctgct gttcaacacc aacggtttta tgctggaaga
    13981 gaacgtcgtc agcaccaaac cgcgtttcca ctttatcgcg gataaacaaa acgatatttc
    14041 gtcgattgtg gtggaactgg attacccggt agatatcagc gaagtttccc gggtgatgga
    14101 aaacctgctg ctggaatcgg cagataaact gctgcgttac aaagggatgc tgtggattga
    14161 cggcgaacct aaccgcctgc tgttccaggg cgtccagcgc ctctacagcg ccgactggga
    14221 ccgcccgtgg ggcgatgaaa aaccgcatag cacgatggtg tttatcggga ttcagctgcc
    14281 ggaggaagag attcgggcgg cgtttgcggg gttgaggaag taaggggtta tgggccggat
    14341 aaggcgcagc cgcatccggc ctgatatttc actcaaaata gtccatatcc agtttcggca
    14401 ccttcaacaa acgtgaagaa acccctactt cgttttcgat cattaagtgc accaggcgtt
    14461 ccccatcaac caacaccata ccctcgacgg attgagcaaa gtcacgcgcc tgagacgtaa
    14521 atccagaagt ggtaataaac accccacgtt tcgccttttg cccagccagt gcgccgtaaa
    14581 atgcctgtaa ttctggcctg cctacagtgt tctgccaacg ttttgcctga acataaactt
    14641 tctccaggcc aagtttatca agcgatatca caccatcgat gccaccatct ccagtaccgc
    14701 caacacgctg caaatcatca cggtggccgc cataccctag gcgatgcaaa acatccagaa
    14761 caatgacttc aaagcgcgaa ggagaaacct gcaataagtt ttccagaacc tcatcagcca
    14821 ccgcatcacg aagctctttt agcgcctgat ctaaccgatc gtccgggctg ctctttgcaa
    14881 gttcttcatg atcgggagag tcggctttcg gatctaaatc gacggcatcc ggccgtgact
    14941 taagtttgac attcacaaaa tcgaaggcca gatggttcgt ctcctgctcc gtcattggct
    15001 ggggatgaga cgcaacccag tcaaaacccg caggagtcag gcaccatttg ccacgcgaca
    15061 aactttgcga caacccggca cgttttaaac ggtcatgcgc ccagcctgca cgatttttat
    15121 aaacaagttg tccgctggta atgactttcg ctcgctggct gtcatccagt cctaatgcat
    15181 ccgcggcagc ctcatgaaca tcacgcgcgg ctgcaccttc cggttttgtc gccagataac
    15241 gcagaacagg ttcaataaat ttgtcatagg taggaaccgt catagtacat ccttgcagaa
    15301 tcaggtagat gtttttcggc tactataaca ctaccaaaat agacgaacac gttagaaatg
    15361 agtcagttgc tgtgaccgtg gtcattgccc ggaaaggtac agaaagctaa gataaggcgt
    15421 tatgggcctt aaatatttgg acaggcccac acagcaatgg attaataaca atgatgaata
    15481 aatccaattt tgaattcctg aagggcgtca acgactttac ctatgccatc gcctgtgcgg
    15541 cggaaaataa ctacccggat gatcccaaca cgacgctgat taaaatgcgt atgtttggcg
    15601 aagccacagc gaaacatctt ggtctgttac tcaacatccc cccttgtgag aatcaacacg
    15661 atctcctgcg cgaactcggc aaaatcgcct tcgttgatga caacattctc tctgtattcc
    15721 acaaattacg ccgcattggt aaccaggcgg tgcacgaata tcataacgat ctcgacgatg
    15781 cccagatgtg cctgcgactc gggtttcgcc tggctgtctg gtactaccgt ctggtcacta
    15841 aagattatga cttcccggtg ccggtgtttg tgttgccgga acgtggtgaa aacctctatc
    15901 accaggaagt gctgacgcta aaacaacagc ttgaacagca ggtgcgagaa aaagcgcaga
    15961 gtcaggcaga agtcgaagca caacagcaga agctggttgc cctgaacggc tatatcgcca
    16021 ttctggaagg caagcagcag gaaaccgaag cgcaaaccca ggctcgcctt gctgcactgg
    16081 aagcacagct cgccgagaag aacgcggaac tggccaagca gaccgaacag gaacgtaagg
    16141 cttaccacaa agaaattacc gatcaggcca tcaagcgtac actcaatctt agcgaagaag
    16201 agagccgctt cctgattgac gcgcaactgc gtaaagcagg ctggcaggcc gatagcaaaa
    16261 ccctgcgctt ctccaaaggc gcgcgcccgg aacccggcgt caataaagcc attgccgaat
    16321 ggccgaccgg gaaagatgaa acgggtaatc agggctttgc ggattatgtg ctgtttgtcg
    16381 gtctcaaacc catcgcggtg gtagaagcga aacgtaaaaa tatcgacgtt cccggcaagc
    16441 tcaatgagtc gtatcgctac agtaaatgtt tcgataatgg cttcctgcgg gaaaccttgc
    16501 ttgagcacta ctcaccggat gaagtgcatg aagcggtgcc ggagtatgaa accagttggc
    16561 aggacaccag cggccaacaa cggtttaaaa tccccttctg ctactcgacc aacgggcgcg
    16621 aataccgcgc agcgatgaag actaaaagtg gcatctggta tcgcgacgtg cgtgataccc
    16681 gcaatatgtc gaaagcctta cccgagtggc atcgcccgga agagttgctg gaaatgctcg
    16741 gcagcgaacc gcaaaaacag aatcagtggt ttgccgataa tcccggcatg agcgagctgg
    16801 gcctgcgtta ttatcaggaa gatgccgtcc gcgcggttga aaaggcaatc gtcaagggtc
    16861 aacaagagat cctgctggcg atggcgaccg gtaccggtaa aacccgcacc gcaattgcca
    16921 tgatgttccg cctgatccag tcccagcgtt ttaaacgcat tctcttcctt gtggatcgcc
    16981 gttctcttgg cgagcaggcg ctgggggcgt ttgaagatac gcgtattaac ggcgacactt
    17041 tcaacagcat tttcgacatt aaagggctga cggataaatt cccggaagac agcaccaaaa
    17101 ttcacgttgc caccgtacag tcgctggtga aacgcaccct gcaatcagat gaaccgatgc
    17161 cggtggcccg ttacgactgt atcgtcgttg acgaagcgca tcgcggctat attctcgata
    17221 aagagcagac cgaaggcgaa ctgcagttcc gcagccagct ggattacgtc tctgcctacc
    17281 gtcgcattct cgatcacttc gatgcggtaa aaatcgctct caccgccacc ccggcgctgc
    17341 atacggtgca gattttcggc gagccggttt accgttatac ctaccgtacc gcggttatcg
    17401 acggttttct gatcgaccag gatccaccga tccaaatcac cacccgcaac gcccaggaag
    17461 gggtttatct ctccaaaggc gaacaggtag agcgcatcag cccgcagggg gaagtgatca
    17521 acgacaccct ggaagacgat caggattttg aggtcgccga ctttaaccgt ggcctggtga
    17581 tcccggcgtt taaccgcgcc gtctgtaacg aactcaccaa ttaccttgac ccgaccgggt
    17641 cacaaaaaac gctggtcttc tgcgtcacta atgctcatgc cgatatggtg gtggaagagc
    17701 tgcgtaccgc attcaagaaa aagtatccgc aactggagca cgacgcgatc atcaagatca
    17761 ctggtgatgc cgataaagac gcgcgcaaag tgcagaccat gatcacccgc ttcaataaag
    17821 agcggctgcc caatattgtg gtaaccgtcg acctgctgac gaccggcgtc gatattccgt
    17881 cgatctgtaa catcgtgttc ctgcgtaaag tacgcagccg tattctgtac gagcagatga
    17941 aaggccgcgc cacgcgctta tgcccggacg tgaataaaac cagctttaag attttcgact
    18001 gcgtcgatat ctacagcacg ctggagagcg tcgacaccat gcgtccggtg gtggttcgcc
    18061 cgaaggtgga actgcaaacg ctggtcaatg aaattaccga ttcagaaacc tataaaatca
    18121 ccgaagcgga tggccgcagt tttgcctagc acagccatga acaactggtg gcgaagctcc
    18181 agcgtatcat cggcctggcc acgtttaacc gtgaccgcag cgaaacgata gataaacagg
    18241 tgcgtcgtct ggatgagcta tgccaggacg cggcgggggt gagctttaac ggcttcgcct
    18301 cgcgcctgcg ggataaaggg ccgcactgga gcgccgaagt ctttaacaaa ctgcctggct
    18361 ttatcgcccg tctggaaaag ctgaaaaccg acatcaacaa cctgaatgat gcgccgatct
    18421 tcctcgatat cgacgatgaa gtggtgagcg taaaatcgtt gtacggtgat tacgacacgc
    18481 cgcaggattt cctcgaagcc tttgactcgc tggtgcaacg ttccccgaac gcacaaccgg
    18541 cattgcaggc ggtgattaat cgcccgcgcg atctcacccg taaagggctg gtcgaactcc
    18601 aggagtggtt tgaccgccag cactttgagg aatcttcctt gcgaaaagca tggaaagaga
    18661 cgcgcaatga agatatcgcc gcccggctaa ttggacatat tcgccgcgct gcggtgggcg
    18721 atgcgctgaa accgtttgag gaacgtgtcg atcacgcgct gacgcgcatt aagggcgaaa
    18781 acgactggag cagcgagcaa ttaagctggc tcgctcgttt agcgcaggcg ctgaaagaga
    18841 aagtggtgct tgacgacgat gtcttcaaaa ccggcaactt ccaccgtcgc ggcgggaagg
    18901 cgatgctgca aagaaccttt gacgataatc tcgataccct gctcgataaa ttcagcgatt
    18961 acatttggga tgaactcgcc tgacacggta tcattcccgg caatttcttc ttctgcggcc
    19021 cgataaagtt gggccgctgc ttaatttacg gaactcacaa tgaacaataa cgatctggtc
    19081 gccaagctgt ggaagctgtg tgacaacctg cgcgatggcg gcgtttccta tcaaaactac
    19141 gtcaatgaac tcgcctcgct gctgtttttg aaaatgtgta aagagaccgg acaggaagcg
    19201 gaatacctgc cggaaggcta ccgctgggat gacctgaaat cccgcatcgg ccaggagcag
    19261 ttgcagttct accgtaacct gctggtacat ctgggcgccg acaatcaaaa gctggtgcag
    19321 gcggtgttcc agaacgtcaa caccaccatt acccagccaa aacagttgac cgaactggtc
    19381 agcaatatgg attcgctgga ctggtacaac ggtgcgcacg gtaagtcgcg cgatgacttc
    19441 ggcgatatgt acgaagggct gttgcaaaaa aatgcgaatg aaaccaagtc tggcgcgggc
    19501 cagtacttca ccccgcgtcc gctgattaaa accattattc atctgctgaa accgcagccg
    19561 cgtgaagtgg tgcaggaccc ggcggcaggt acggcgggct ttttgattga agccgaccgc
    19621 tacgttaagt cgcagactaa cgatctggac gaccttgatg gcgacacgca ggatttccag
    19681 atccaccgcg cgtttatcgg cctcgaactg gtgcccggca cccgtcgtct ggcgctgatg
    19741 aactgcctgc tgcacgatat tgaaggcaac ctcgaccacg gcggcgcaat ccgtctgggc
    19801 aacaccctgg gcagcgacgg tgaaaacctg ccgaaggcgc atattgtcgc cactaacccg
    19861 ccgtttggca gcgccgcagg caccaacatt acccgcacct ttgttcaccc gaccagcaac
    19921 aaacagttgt gctttatgca gcatattatc gaaacgctgc accccggcgg tcgtgcggcg
    19981 gtggtggtgc cagataacgt gctgtttgaa ggcggcaaag gcaccgacat tcgtcgtgac
    20041 ctgatggata agtgtcatct gcacaccatt ctgcgtctgc cgaccggtat tttttacgcg
    20101 cagggcgtga agaccaacgt gctgttcttt accaaaggca cggtggcgaa cccgaatcag
    20161 gataagaact gcaccgatga tgtgtgggtg tacgacctgc ggaccaatat gccgagcttc
    20221 ggcaagcgta cgccgtttac tgacgaacat ctgcaaccgt ttgagcgcgt gtatggcgaa
    20281 gacccgcacg gcttaagccc gcgcactgaa ggtgaatgga gttttaacgc tgaagagacg
    20341 gaagttgccg acagcgaaga gaacaaaaac accgatcagc atctggccac cagccgctgg
    20401 cgcaagttta gccgcgagtg gatccgtacc gcgaaatccg attcgctgga tatctcctgg
    20461 ctgaaagata aagacagtat tgatgccgac aacctgccgg agccggatgt attagcggca
    20521 gaagcgatgg gcgaactggt acaggcactg ggtgaactgg atgcgctgat gcgtgaactg
    20581 ggggcgagtg atgaggctga tgcacagcgt cagttgctgg aagaagcgtt tggtggggtg
    20641 aaggaatgag tgaaaaaacg aggccagaat catggctgtc aaaaatagcc attgattgct
    20701 ttgatcaaat ttcaaccaca aacaataaag taaaaagtac tgcttgttca cctcatgggt
    20761 tatatcctgt aattgaccaa gggcaaaatt atatctctgg atatattaat gatgagagta
    20821 aacttatcaa aataactaca cctatttgta tatttggcga tcacaccagg atcatcaaat
    20881 gggtggaaca taatttcgtg cctggcgctg atggtactaa attactaaaa cctaaggatt
    20941 ttatttaccc tagatatgca tattacagcc tttgcgctct gaatattcca gataagggat
    21001 actcacgcca cttcaagtat ttcaaagagt taaagattga tattcctgca tgggaagaac
    21061 aaaaaatcat cgctgaaaaa ctcgatacgc tgctggcgca ggtagagagc accaaagcac
    21121 gtcttgagca aatcccacaa atcctgaaac gttttcgtca ggcggtgctg gggacggcga
    21181 tgagtgggaa cttaactggg gaatggaggc tgtccaatca ttccatcatt ctcgcagaag
    21241 aaatcgaaga aaacaaaaat agattgattg aaaaaaaatt agttaagaaa gatttaacat
    21301 acgctagtct tgataaaaga tattcgctac ctccaagttg gctatatatt aagctacaat
    21361 cagtttccac taaaattact gacggtaaac ataaaacgcc caaaagagaa actgcaggaa
    21421 agttcttact ttcagcaaga aatattcaag atggttatat taaactggct gatgttgatt
    21481 atgttggcga tgctgaattt caaaaattac ggaataggtg cgaccctgat tacggggata
    21541 ttcttatctc ctgctctggt tcaataggac gggtatgtct tgttgatgaa aactcaaaat
    21601 atgttatggt tcgtagtgtt gcattagtaa aattaatgca agattttgtt atcaataaat
    21661 atatgatgtt cctacttcaa tcaccattat tacaaaaaga aatagaggaa aattcaaaat
    21721 caactgccca atcaaattta tttcttgggc caattaaaaa tcttggaatt cccctacctc
    21781 cggtatcaga acaacacgaa atcgttcgcc gcgtcgagca actcttcgcc tacgccgaca
    21841 ccatagaaaa acaggtcaac aacgccttag cccgcgtcaa caatctgaca caatccatcc
    21901 tggcaaaagc gttccgtggt gaactcaccg cccagtggcg ggccgaaaac ccggaattga
    21961 tcagcggaga aaacagcgcc gccgcgttgc tggagaaaat caaagctgaa cgcgcagcca
    22021 gcgggggtaa aaaagcctcg cgtaaaaaat cctgaacatt attttctggc gcacctttcc
    22081 ggtgcgcttt taattatttc acgccaatca taattcacgc aaatatattt aaatcactcc
    22141 agaaattgcc cattttattc tatttttagc tggactttcc ctgtatttac tgatgatata
    22201 tccaggtatt tagcgcggtg cggatgtgcg ccaacacacc cgcaccgctc atcacaatca
    22261 ctatttatgg agaatagtaa tcatgactga cacgcattct attgcacaac cgttcgaagc
    22321 agaagtctcc ccggcaaata accgtcaatt aaccgtgagt tatgcgagcc gctacccgga
    22381 ttacagccgt attcccgcca tcaccctgaa aggtcagtgg ctggaagtcg ccggttttgc
    22441 caccggcacg gtggtagatg tcaaagtgat ggaaggctgt attgtcctca ccgcccaacc
    22501 acccgccgcc gaagagagcg aactgatgca gtcgctgcgc caggtgtgca aactgtcggc
    22561 gcgtaaacaa aagcaggtgc aggcgtttat tggggtgatt gcgggtaaac agaaagtcgc
    22621 ctgatacatt gacccggtag caccagtgct accgggtatt attcgccgcg ttataccaac
    22681 gcgctctcca tttgtttttc aaatgcctga cgaattaatc ccataatgta aggaagttgt
    22741 gcgaggtcgc tgaaaccaat ttccacatcg ccattacccc agcgcccaac atttgtcaca
    22801 tctttggcaa tacctttagg atcgaccagt tcatgaaact gcatattaag cgacaatcgc
    22861 agtcggcttt tttgcggcac aacatcaaca aaattcgttt cagctttaaa cgcaatatag
    22921 agcttcaata cttcctgcgt gatccctgcg tccaggcgca taatttcatc gcgaagatga
    22981 tcaaataaca accggctatg caagccatca gcaagaaaag gataatcact caggctgtaa
    23041 ctggttttgt tttcaggctg tccccgataa acagccaaaa cctcttcagg aagagaaggc
    23101 gcttgccaga cctgaaccgc gagatcggcc agacgatcgg cacgggcacg aatggcggct
    23161 tcatcccatt tttcgcactg tccaagaccg atattcaaat agagcgggct atgtttaaag
    23221 ccatcttcaa tatcgcgttt ttccgcgaaa ggtctgtcac tgtagcggga gttatagcgc
    23281 gttaatgtga gattccccaa cgtatgcagc agttctttat gcacacgctg ccagtcactt
    23341 cccagctctt cacgccattt agccgacaaa ttttcgttct gcggcataat gtgctcaatc
    23401 gtaaactctt ccacgcgctc tctgcgttta tcgttttcca gtcgtcgtaa ccagtagctg
    23461 cgactacgga agttatagag atcgcgaact tttaattccc gtttaaattc atcatcgttg
    23521 gggaaacgac gatatgaagg tagattcaaa aaatgtacct gaatactttc cagataattt
    23581 tctttattaa taactttata aaaagtggca aatgtcttgt tcaaagaatt tgtcggaatt
    23641 gcatatactg cacggcggaa aacataagat tcaattaaac gaattatgct caggaaatcc
    23701 tcgtgagaca aaacgccatt tttatagtca tgataaagcg ccagtaagaa aggatacgcc
    23761 acatcgacct ttaactcgcg caaatcctga aaagccgtag caagcgattt gtcactttct
    23821 tttcccaatg ccattgcaca gtaatactcc gcgtagatat gaatatcttc aaccagccga
    23881 tctacgcctt tttcagcaac actctggctg cgggcatgga gtttaaatgc ctcatagact
    23941 tcatctgtcc gagggatctc ccccgttttt accgtcagat aatgacgcat aaaactgtca
    24001 aaatattcgc tgtaaccttg ctgaccaaag gcgacttcca ttggacgcca gtgatcttca
    24061 tacaatcggg tttgatgctc tggttcgagg cccatcagaa taaaattgcg caccagatcg
    24121 gcctgactta acgccttacc ggtggagttc atactttcaa aaatcagttg cggattatcc
    24181 tgaccacgat taagcgccac atcgacaatt aataactttg ctaacccacg acataagggg
    24241 atcaagtcat caccgagttt ggcaatttgc tcatcaaaga aagtaaagtt ttccattatt
    24301 cgatgggaat agttttctgg cattggtctt tgttttatta acgccagtaa actgtcttta
    24361 tcagtctcgg tcagcagtaa tttaaaacct ttctcgccgg actcataagg attgagcaaa
    24421 taataattac gcaatttcat tgccgaaaag ccatcaaaaa cttcgtcttc gccaagatgg
    24481 cgcgataaag cctcaatcag caacattgcg gtcgtcagcc gctgctgacc atcaatgacc
    24541 agcaacggag aaataccaga aacctgatac aatccctgct ctatataaac aaccgaaccg
    24601 ataaaatgcg ctgatatatc gtcacgcttt ccggcacgaa tgatgtcgtc ccaaagttgc
    24661 cgacattgtt gcccggtcca tgaataagtg cgttgataaa tgggaataac aaactgctgc
    24721 gaacgtttta aaaaatcgag taggcgagcc tctgtggctt tcatcctggc gtccttccgt
    24781 gaagcaataa tgacgcaata ataaccactt ccttgtgaag gttatagcga aatccggttt
    24841 atccgcgacg aacatacccc tcactccaca tcctccctgc gtaacccgat atccctcaac
    24901 cgttcatcac tcatctgctg taacacccgt cgggcctgca tttgccgccg ccaacgcctc
    24961 accgcctgcc agagttgtac aagtctgata aacggcgttt tggctttgtt ttcgtgaaat
    25021 tccatctgct gcctcctcat ctttcagagg cttcatcatc cgtcttctgg cagttcacaa
    25081 tacagatgca aatattagtt ttatttaaca tacagacagg ttaaaacagc atctgcatcc
    25141 ggttttttag cttcatctgt accggttttt ctctctgtat ggtctaaata aggaatacag
    25201 catgacgcgt tatcaacatc tggcgactct acttgccgag cggattgagc aagggctgta
    25261 tcgtcacggg gagaaattgc cgtcggtgcg cagcttaagt caggagcacg gcgtcagcat
    25321 cagcaccgtg cagcaggcgt accagacgct ggagacgatg aagctcatca ctccgcagcc
    25381 gcgttcgggt tattttgtcg cacaacgtaa agcccagccg ccagtgccgc cgatgacgcg
    25441 tccggtgcag cgcccggtgg aaattaccca gtgggatcag gtgctggata tgctggaggc
    25501 gcatagcgac agttccattg ttccgttaag caaaagcacg ccggatgtcg aaacgcccag
    25561 cctgaaaccg ctgtggcgtg agctaagccg ggtggtgcag cataatctgc aaaccgttct
    25621 cggttatgac ttgttagccg gtcagcgggt attgcgagag cagattgccc gcctgatgct
    25681 cgacagcggc tcggtggtca ccgccgatga catcatcatc accagcggct gccataactc
    25741 gatgtcgctg gcgttaatgg cggtgtgtaa accgagcgat attgtcgcgg tcgaatctcc
    25801 ctgttattac ggttcgatgc agatgctgcg cggcatgggc gtgaaagtga ttgaaatccc
    25861 aaccgatcca gaaactggca tcagcgttga agcgctggaa ctggcgctgg aacagtggcc
    25921 gattaaaggc atcattctgg tgccaaactg taataatccg ctgggattta ttatgccgga
    25981 cgcgcgcaaa cgggccgttc tctctctcgc tcagcgtcat aatattgtga tttttgaaga
    26041 tgatgtctac ggcgaactgg caacggagta tccgcgcccg cggaccattc attcatggga
    26101 tatcgacggg cgagtgctgt tgtgcagctc gttcagtaaa agtattgcac ctggcctgcg
    26161 cgtgggctgg gtcgcaccgg ggcgctatca cgataaactg ctgcatatga aatacgccat
    26221 cagcagcttt aatgtaccgt ccacgcaaat ggcggcggca acgtttgtgc tggaaggtca
    26281 ctatcatcgc catatccggc ggatgcgaca gatctatcag cgcaatttgg cgctttatac
    26341 ctgctggata cgggaatatt ttccctgcga aatctgtatt acgcgcccga aaggcggatt
    26401 tttactgtgg atagaattgc ctgaacaggt cgatatggtc tgcgtcgcgc ggcagctgta
    26461 ccgcatgaaa atccaggtgg cggcaggctc gattttctcg gcttccggca aataccgtaa
    26521 ttgtctgcgc atcaactgcg ctttgccgct cagcgaaacc tatcgcgaag cactgaagca
    26581 aattggcgag gccgtgtatc gggcaataga ataatttcga attagtggcg aggcccctga
    26641 tttcctctca cgaatcgcct gtgactcgtt ttatctttgc aggggcatcg ccacgttcca
    26701 gcatggctta ggcaaagtgt gctttgttca taccggatgc ggcgtgaacg ccttatccgg
    26761 cttacaaatc gcgcaaattc aatagattgc agaggtaatg taggtctgat aagcgtagca
    26821 catcaggctg ctttgcgttt gtcatcagtc tctgattagt actcgatcca tggatcgcgc
    26881 ttgctttcag gcaagggttg ctctaatgcc tgcatttgct ctgccgaaag tccagtatat
    26941 cgttgtatcc attcaggatc cgcaccgttc tgcaaaagca atctaaggag gcgttgttcc
    27001 ccttcttctt tccccttttc catccctaac agccatccat cattatgaat tcgctctgca
    27061 atcgtcatta ttcgctccct ttgttgtggc attcgacggg taagttcact gataaactca
    27121 ttaaaacgcg cttcatcgcc agtcagtaaa atgtaattta acagcgcagt tatctggctg
    27181 tcattagcac actctgtaac cagtaatatt accagttgat cgataagccc catcagatcg
    27241 cgctggcgaa tatgcttttg gatcaactcc aacagggcga ctctgcggtg ctgcacaatc
    27301 tcgtcgtctg gcacgacagt gacatccacc agcgggaacg ctgcagtata aagtttccgt
    27361 gcagtagtcg ggtcggcaaa ttcgtccagc cagcacaggg accaggggta aggactacgg
    27421 ctaccgtgat aaaacagcat cgggatgacc agcggtagct gccggcgttt atcatgctcg
    27481 atatggcgct gcatcaccgc catggaatat cgcatcaggc gaaaggccat atgaatatcc
    27541 tcacggctct gatgttcaat caccacataa ataagccatc tccttcgcgg gtctttaccg
    27601 accacagaat atcggaatgt agcgcccgca atttttcatc gacaaagctg gcggattcca
    27661 gttttaagct gtcgagatcg cacagttcac gtaaatcttt gggtaagtgg atctccataa
    27721 aatcccgcgc ggtgtcaggg tgcatgagaa aagatttaaa taatgcgtca tgcggcgtgc
    27781 tggtcgtgaa gtttgtcatg gcgatccgtc acctgtagat aagatggccc gactttagcg
    27841 actaactaaa ccattctcac ccggcaattt ctcgttcccc gaggcgcttt ccaggctatt
    27901 tttattcttc tgcaaacgat acatttttcc ggaaacaggc tcgcgtttta catttcctgt
    27961 acccggcaga tattcccctt tccaccgcgc tcttttgatt aaccgcaaat tttaattaca
    28021 cttaaggtgt atattttcta tgcaacccat caattaaaga ggtgtaatgt gctgatgact
    28081 atttgtaatc gttatacatt ctgacctgga gtcagaaggt atttctctgt ctgtgtgttc
    28141 acaggcagtg tggttgatta catgaaattc agtacatttg tccttctcgc ctacctttcg
    28201 tcattaccga cggtattgaa tttcgttttc cccgttgggg ttctccggac aaggagttgt
    28261 ttgttatgcc acgttttttt gcccgccatg ccgctacgct gtttttcccg atggcgttga
    28321 ttttgtatga ctttgccgcc tatctttcga cggatctgat ccagccgggg atcattaatg
    28381 tggtccgtga ttttaatgcc gatgtcagtc tggctccggc tgccgtcagt ctctatctcg
    28441 ctggcggtat ggcgttgcag tggctgctgg ggccgctttc cgacaggatt ggccgcaggc
    28501 cagtgctgat taccggggcg ctaatattta cccttgcctg cgctgcgaca atgttcacaa
    28561 cgtctatgac acagtttctt atcgcgcgtg caattcaggg caccagtatc tgttttattg
    28621 ccaccgttgg ttatgtcacg gtgcaggagg cgttcggacg gacaaaaggg atcaagttga
    28681 tggcgattat cacctccatc gtactgattg cgccgattat cggcccgctt tccggcgcag
    28741 ctctgatgca ctttgtgcac tggaaagtcc tttttgccat cattgcggtt atgggtttta
    28801 tctcatttgt tggcttactg ctggcgatgc cagagacggt gaagcgcggc gcggttccat
    28861 ttagcgccaa aagcgtcttg cgcgattttc gtaatgtctt ttgcaatcgg ctgttcctct
    28921 ttggcgcagc aaccatctct ttaagctata tccccatgat gagttgggtg gctgtctcgc
    28981 cggtgatcct gattgacgca ggcggcttaa cgacctcgca gttcgcctgg acacaggttc
    29041 cggtgttcgg cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt aaagatccga
    29101 ccgaaccgcg ctttatctgg cgtgccgtac ccattcaact ggttggcctc gcgctgttga
    29161 ttatcggcaa tctgctgtca ccgcacgtct ggctgtggtc gatgctgggc accagtctgt
    29221 atgctttcgg gattggtttg attttcccga ccttattccg ctttacgctg ttttccaata
    29281 acttaccgaa agggaccgtc tccgcatcgc tgaatatggt gatcctgatg gtgatgtctg
    29341 tctcggtcga aatcggccgc tggctgtggt ttaacggcgg tcgcttgccg tttcatctgt
    29401 tagccgtcgt ggcgggcgtt atcgtcgttt tcaccctggc aggattgctt aatcgcgtgc
    29461 gccaacatca ggctgccgag ctggcggagg agcggtgatt tttgcgcgat cctgccgtca
    29521 ggctctattc ttaacgttat gaataaactc attgaactca gacgcgccaa aatgttggcg
    29581 ctctctttac tgcttatcgc cgctgctacc tttgtcgtta cgctgttttt gccgcccaat
    29641 ttttgggtga gcggtgtgaa ggcgattgcc gaagcggcga tggtcggcgc gctggcggac
    29701 tggtttgcgg tggtggcgct gtttcgccgc gtgccgattc cgatcatttc tcgtcataca
    29761 gcgattatcc cgcgtaataa agaccggatt ggcgaaaatc tcggccagtt cgtgcaggaa
    29821 aaatttcttg atactcaatc cctggtggca ttgattcgac gccacgaacc ggcgttgttg
    29881 attggcaact ggtttagtca gccagaaaac gcccgccgcg ttggtcagca tctgttgcag
    29941 atcatgagcg gttttctcga attaactgat gatgcgcgta ttcagcgcct gcttaagcgc
    30001 gcggtccatc gggcgattga taaggtcgat ctttccggca ccagtgcgtt gatgctggag
    30061 agtatgacca aaaacgatcg tcatcaggtg ctgctggata cgctgatcgc acagttgatc
    30121 gcccttctcc agcgcgataa atcgcgcaag tttatcgccc agcaaattgt tcgctggctg
    30181 gagagtgagc atccactgaa agccaaaatt ctccccaccg aatggctggg cgaacatagc
    30241 gcggagttgg tttctgacgc ggtgaattct ttgcttgatg atatcagccg cgatcgtgcg
    30301 catcagatcc gccatgcgtt tgatcgcgcc acttttgccc tgatcgacaa gctgaaaaac
    30361 gatccggaaa tggcagcgcg agccgatgcc gtaaaaagtt atctgaaaga agatgaagct
    30421 tttaaccgct atctcagtga attgtggggg gatttacggg aatggctgaa agcggatatc
    30481 gacagtgaag attctcgtgt gaaagaacgt atcgcgcggg cgggtcaatg gtttggcgaa
    30541 acgttaattg ccgatgatgc cttgcgggcg tcgttaaatg gtcatctgga acaagccgcg
    30601 caccgcgtcg cgcctgagtt ttccgcattc ctgacgcgcc acatcagcga tacggtaaaa
    30661 agctgggatg cccgggatat gtcgcggcaa atagagttaa atatcggcaa agatctgcag
    30721 tttattcgtg tcaacggtac gctggttggc ggttgtattg ggctaatttt atatttgctg
    30781 tcgcagctcc cggccttgtt ccccctcggc aatttttaga aatccatgaa aaactaagtg
    30841 gctaaccgcg agagagatca aataaaccat actaaaagaa tggtaataat gtggcctctt
    30901 ttcattatct cccgtggtac ggggaaggaa aatcatgtca cttgtcaccg atctacccgc
    30961 cattttcgat cagttctctg aagctcgcca gaaaggcttt ctcaccgtca tggatctcaa
    31021 ggagcgcggc attccgctgg ttggcactta ctgcaccttt atgccgcaag agatcccgat
    31081 ggcagccggt gcggttgtgg tttcgctctg ttccacctct gatgaaacca ttgaagaagc
    31141 ggagaaagaa ctgccgcgca acctctgccc gctgattaaa agcagctacg gcttcggcaa
    31201 aaccgataaa tgcccctact tctacttttc ggatctggtg gtcggtgaaa ccacctgcga
    31261 cggcaaaaag aaaatgtatg aatacatggc ggagtttaag cctgttcatg tgatgcagct
    31321 gccgaacagc gttaaggacg atgcctcgcg tgcattgtgg aaagccgaga tgctgcgctt
    31381 gcaaaaaacg gtagaagaac gttttgggca cgagattagc gaagatgctc tgcgcgatgc
    31441 tattgcgctg aaaaaccgcg aacgtcgcgc actggccaat ttttatcatc ttgggcagtt
    31501 aaatcctccg gcgcttagcg gcagcgacat tctgaaagtg gtttacggcg caaccttccg
    31561 gttcgataaa gaggcgttga tcaatgaact ggacgcgatg accgcccgca ttcgtcagca
    31621 gtgggaagaa ggccagcgac tggacccgcg tccgcgcatt ttaatcaccg gctgcccgat
    31681 tggcggcgca gcagagaaag tggtgcgcgc gattgaagag aatggcggct gggttgtcgg
    31741 ttatgaaaac tgcaccgggg cgaaagcgac cgagcaatgc gtggcagaaa cgggcgatgt
    31801 ctacgacgcg ctggcggata aatatctggc gattggctgc tcctgtgttt cgccgaacga
    31861 tcagcgcctg aaaatgctca gccagatggt ggaagaatat caggtcgatg gcgtagttga
    31921 tgtgattttg caggcgtgcc atacctacgc ggtggaatcg ctggcaatta aacgtcatgt
    31981 gcgtcagcag cacaacattc cttatatcgc tattgaaaca gactactcca cctcggatgt
    32041 cgggcagctc agtacccgtg tcgcggcctt tattgagatg ctgtaaggag tggcagtgac
    32101 atattcgatt ggcattgatt ctggttctac cgccaccaaa gggatcttac tggcagacgg
    32161 cgtgattacg cgccgtttcc tcgttccaac accctttcgt ccggcaacag caattactga
    32221 agcctgggaa actctgcgcg aagggttaga gacaacgccg tttctgacgc tcaccggcta
    32281 cgggcggcaa ctggtggatt ttgccgataa acaagtgacg gaaatctcct gtcacgggct
    32341 gggcgcacgg tttcttgcgc cagcaacgcg cgcggtaatc gacatcggtg gtcaggacag
    32401 caaagtgatt cagcttgatg atgacggtaa cctgtgcgat ttcctgatga atgacaaatg
    32461 cgcggcgggc accgggcgtt tcctggaggt gatctcgcgc acgcttggca ccagcgtcga
    32521 gcaactcgac tgcattaccg aaaatgtcac gccgcacgcc atcacgagta tgtgcacagt
    32581 gtttgctgaa tcagaagtga tcagcctgcg ctcagcgggc gtcgcgccag aagcgattct
    32641 cgccggagtg attaacgcga tggcgcggcg gagtgccaat ttcattgctc gtctctcctg
    32701 tgaagcgccg attctgttta ctggtggcgt tagtcattgc cagacgtttg cccggatgct
    32761 ggaatctcac ctgcgaatgc cggtaaatac ccatcctgat gcgcaatttg ctggcgcaat
    32821 tggcgcggcg gtaattggtc aacgagcgag aaaacgccga tgaaagagtt tttattttta
    32881 tttcactcca cggtcggcgt catacaaacc cgcaaggcat tgcaggcagc gggcatgact
    32941 tttcgcgtca gcgatattcc ccgtgattta cgcggcggct gcgggttatg catttggctg
    33001 acctgtcccc ccggcgagga aatacaatgg gtgatccctg ggcaaaccga agcgatatat
    33061 tgccagcagg gcagcgactg gcagtgcgtg gtgcattacg atgcagaagc gtcaaccagg
    33121 caataggatc tttaaatata ccaaccggat aattggctgc gcataatcct gcatgaagga
    33181 agttgtatta aatatctcac ctgccatgaa atatttccca tccacaaaat gagcgatttt
    33241 tgatagcacc ttactatttt acgtctaaaa tacaaaatac attttattat acttaaaccc
    33301 ataattatgc atagagaaat aaaatgatta ccgggtgaga taatgataaa agataagttt
    33361 ctgtgcgaag cttatcttta cccatccccc tgattatttt agattt
//
LOCUS       DAZFFG010000041        32148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFFG010000041 DAZFFG010000000
VERSION     DAZFFG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.041"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(65..1825)
                     /gene="ycaO"
                     /locus_tag="ACHJ7J_002291"
     CDS             complement(65..1825)
                     /gene="ycaO"
                     /locus_tag="ACHJ7J_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO5000520.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1955..2647
                     /locus_tag="ACHJ7J_002292"
     CDS             1955..2647
                     /locus_tag="ACHJ7J_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO5000521.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2846..3934
                     /gene="serC"
                     /locus_tag="ACHJ7J_002293"
     CDS             2846..3934
                     /gene="serC"
                     /locus_tag="ACHJ7J_002293"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO5000522.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4005..5288
                     /gene="aroA"
                     /locus_tag="ACHJ7J_002294"
     CDS             4005..5288
                     /gene="aroA"
                     /locus_tag="ACHJ7J_002294"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO5000523.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALAALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5457..6221
                     /gene="ycaL"
                     /locus_tag="ACHJ7J_002295"
     CDS             5457..6221
                     /gene="ycaL"
                     /locus_tag="ACHJ7J_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO5000524.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6394..7077
                     /gene="cmk"
                     /locus_tag="ACHJ7J_002296"
     CDS             6394..7077
                     /gene="cmk"
                     /locus_tag="ACHJ7J_002296"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO5000525.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKTLQ
                     YARQKLALA"
     gene            7188..8861
                     /gene="rpsA"
                     /locus_tag="ACHJ7J_002297"
     CDS             7188..8861
                     /gene="rpsA"
                     /locus_tag="ACHJ7J_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO5000526.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9021..9305
                     /gene="ihfB"
                     /locus_tag="ACHJ7J_002298"
     CDS             9021..9305
                     /gene="ihfB"
                     /locus_tag="ACHJ7J_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO5000527.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9512..11776
                     /locus_tag="ACHJ7J_002299"
     CDS             9512..11776
                     /locus_tag="ACHJ7J_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO5000528.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11813..13561
                     /gene="msbA"
                     /locus_tag="ACHJ7J_002300"
     CDS             11813..13561
                     /gene="msbA"
                     /locus_tag="ACHJ7J_002300"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO5000529.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13558..14544
                     /gene="lpxK"
                     /locus_tag="ACHJ7J_002301"
     CDS             13558..14544
                     /gene="lpxK"
                     /locus_tag="ACHJ7J_002301"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO5000530.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14581..15813
                     /gene="ycaQ"
                     /locus_tag="ACHJ7J_002302"
     CDS             14581..15813
                     /gene="ycaQ"
                     /locus_tag="ACHJ7J_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO5000531.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15865..16047
                     /gene="ycaR"
                     /locus_tag="ACHJ7J_002303"
     CDS             15865..16047
                     /gene="ycaR"
                     /locus_tag="ACHJ7J_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO5000532.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16044..16790
                     /gene="kdsB"
                     /locus_tag="ACHJ7J_002304"
     CDS             16044..16790
                     /gene="kdsB"
                     /locus_tag="ACHJ7J_002304"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5000533.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16944..17837
                     /locus_tag="ACHJ7J_002305"
     CDS             16944..17837
                     /locus_tag="ACHJ7J_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO5000534.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17814..18593)
                     /gene="elyC"
                     /locus_tag="ACHJ7J_002306"
     CDS             complement(17814..18593)
                     /gene="elyC"
                     /locus_tag="ACHJ7J_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO5000535.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18729..19514
                     /gene="cmoM"
                     /locus_tag="ACHJ7J_002307"
     CDS             18729..19514
                     /gene="cmoM"
                     /locus_tag="ACHJ7J_002307"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO5000536.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19511..20833
                     /gene="mukF"
                     /locus_tag="ACHJ7J_002308"
     CDS             19511..20833
                     /gene="mukF"
                     /locus_tag="ACHJ7J_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO5000537.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20814..21518
                     /gene="mukE"
                     /locus_tag="ACHJ7J_002309"
     CDS             20814..21518
                     /gene="mukE"
                     /locus_tag="ACHJ7J_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO5000538.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21518..25978
                     /gene="mukB"
                     /locus_tag="ACHJ7J_002310"
     CDS             21518..25978
                     /gene="mukB"
                     /locus_tag="ACHJ7J_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO5000539.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVVVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26239..28086
                     /gene="ldtD"
                     /locus_tag="ACHJ7J_002311"
     CDS             26239..28086
                     /gene="ldtD"
                     /locus_tag="ACHJ7J_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5000540.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28267..28815
                     /locus_tag="ACHJ7J_002312"
     CDS             28267..28815
                     /locus_tag="ACHJ7J_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO5000541.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDLGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28842..29489
                     /gene="gloC"
                     /locus_tag="ACHJ7J_002313"
     CDS             28842..29489
                     /gene="gloC"
                     /locus_tag="ACHJ7J_002313"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO5000542.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29540..30730)
                     /gene="aspC"
                     /locus_tag="ACHJ7J_002314"
     CDS             complement(29540..30730)
                     /gene="aspC"
                     /locus_tag="ACHJ7J_002314"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO5000543.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30915..32003)
                     /gene="ompF"
                     /locus_tag="ACHJ7J_002315"
     CDS             complement(30915..32003)
                     /gene="ompF"
                     /locus_tag="ACHJ7J_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO5000544.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7810 a         7833 c         8665 g         7840 t
ORIGIN      
        1 aaaaaacgcc ccgtaatata actcagacga atcattaagc ctacattgcg taggctattt
       61 gattttattt tgcccagaat gctgctttgg cgcgctgcag cttttcgtag gccttcaaca
      121 gcgactgatg tgcagcaaac gcgtgcagat cgctatctac tggttgcaag ccataaaacg
      181 ccgcttcgcc gctcattgcc gcactggcgg cttccacggc atctgcgccg tacatgcgaa
      241 caaacgcatt cagatattgc agcggctggc gatcttcttc ctgtgccagt aataacaacg
      301 tttgcaggca gcgataatag ttggcgcgtt ccgggctgaa taccgatgag ttaaactcca
      361 tcgtccattc ggtccagacc agagcctgtt ccagatcgcc accagccagc gccagcatgg
      421 cttttaattc accgatacgc agggtgtacc aaccgttatc cgacccggtc gccagaccca
      481 acagctcacg cacgcgggta aagtcatcaa aaccttcttc atccagttgc tcgatgaggt
      541 tcaggtaatc ttctttttcc cactcgctgc ctggtagcga aagaatcgtt tcacgtaaat
      601 ggctgcccat actgttattc gcgagccaca gatcttcagc cggataaata tcggacatgc
      661 caggcacgat aatacggcaa gcataaacgc ccagatgctc gtaatcggca atataaactt
      721 ctttatcttc tttgttgaag atagccatca gcgtagcaaa ctcttcttcc gtggtgccgg
      781 agaaattcca gtccacaaac ggataatcgg catcctgctt gaacaggtcc caggagatta
      841 aaccgctgga atcgataaag tgcgtttcga ggttggtatg ttcagcgact tcttcatcat
      901 cgaaggttgg cggagtaaac acatccaaat ctttcaggcc acgaccttgc agcagctcgg
      961 tcactgtacg ttccagtgct acgccaaaat caggatgcgc accgaaagag gcaaagcagg
     1021 tgccgttagc aggattaaac agtaccacgc aaatcaccgg atactggccg ccaagggaac
     1081 catcatatgc gaagatcggg aaaccttctg cttccagtgt ttcgatcgct tcaaccaccg
     1141 ctgggtaacg cgccagcacg tctgccggga tttccggcaa gctgatgctt tcagcaataa
     1201 tgcggttttt cacgtagcgt tcgaaaactt cggacaaccc ctgaacgcgt gcttcgttac
     1261 gggtattgcc agcagacata ccgttagaaa cgtacaggtt accaatgata ttcatcggaa
     1321 tataaacggt ctgattgtcg gactggcgcg taaacggcag accgcaaata ccacgatctt
     1381 cgttaccgga ttgtaggtca atcagcatac tgccggtcaa ttcattctcc ggatcgtaaa
     1441 atgcgcgcag acggtcatcg agcagccctt ctggcacatc gtcattttcg gtcagtggga
     1501 accatttttc gttgggataa tgcacaaatg gaccgttggc gatggtttcg cccagccaga
     1561 aatcagcaaa aaagtagttg gttgagagac gctcgaaata ttcaccgagt gcagaagcca
     1621 gcgccgcttt cttggttgcg cctttaccgt tggtaaaaca cagtgcgcac tctttgtcgc
     1681 gaatatgtac agaccagacg ttaggcacgg gattcagcca ggaggcctct tcaatctgaa
     1741 agccgaggtc tgaaagtttt tgctggaagc gagcgatgga atcttccagt gcggcatctt
     1801 tgccggggat aaatgtttgc gtcatgaaaa tcactttagt cgtacggaaa gcgcgcaata
     1861 atacgggttt tatctcaaag gcgctatcac cgccgccatg ccgatgaact gttgactatg
     1921 cttttagcgg ataaaccacg ttaagagcat aaaaatgaag gcgttcgatc tccaccgtat
     1981 ggcatttgat aaagtgcctt ttgatttcct tggcgaagtt gcactacgta gtctgtacac
     2041 ctttgtactg gtttttttgt tcctcaaaat gaccggaaga cgcggagtgc ggcagatgtc
     2101 gctgtttgaa gtgttaatca ttctgacgct ggggtcagcg gcgggagatg tggcgtttta
     2161 tgatgatgtg ccgatggtcc cggtacttat cgtctttatc actctggcgt tgttataccg
     2221 cctggtaatg tggttgatgg cacacagtga aaaactggaa gatcttctgg aaggtaagcc
     2281 ggttgtcatt attgaagatg gcgagctggc ctggtcgaaa ctcaataact ccaacatgac
     2341 ggaatttgag ttctttatgg agctacgatt gcgcggtgta gagcagttgg ggcaagtacg
     2401 tctggcgatt ctcgaaacca acgggcaaat cagtgtctat ttctttgaag atgacaaggt
     2461 gaaaccgggt ttacttattt tacccagtga ttgtacgcag cgttacaaag tggtgccgga
     2521 gtcggcggac tatgcctgta ttcgttgtag tgaaatcatt catatgaaag cgggggaaaa
     2581 acaattatgt ccgcgctgtg caaatccaga atggacgaag gcaagtcggg caaaacgggt
     2641 gacctgacag taaaaacatc ggctttttgc taataatccg agagattctt ttgtgtgatg
     2701 caagccacat ttttgccctc aacggtttta ctcattgcga tgtgtgtcac tgaatgataa
     2761 aaccgatagc cacaggaata atgtattacc tgtggtcgca atcgattgac cgcgggttaa
     2821 tagcaacgca acgtggtgag gggaaatggc tcaaatcttc aattttagtt ctggtccggc
     2881 aatgctaccg gcagaggtgc ttaaacaggc tcaacaggaa ctgcgcgact ggaacggtct
     2941 tggtacgtcg gtgatggaag tgagtcaccg tggcaaagag ttcattcagg ttgcagagga
     3001 agccgagaag gattttcgcg atcttcttaa tgtcccctcc aactacaagg tattattctg
     3061 ccatggcggt ggtcgcggtc agtttgctgc ggtaccgctg aatattctcg gtgataaaac
     3121 caccgcagat tatgttgatg ccggttactg ggcggcaagt gccattaaag aagcgaaaaa
     3181 atactgcacg cctaatgtct ttgacgccaa agtgactgtt gatggtctgc gcgcggttaa
     3241 gccaatgcgt gaatggcaac tctctgataa tgctgcttat atgcattact gcccgaatga
     3301 aaccatcgac ggtatcgcca tcgacgaaac gccagacttc ggcaaagatg tggtggttgc
     3361 cgccgacttc tcttcaacca ttctttcccg tccgattgac gtcagccgtt atggcgtgat
     3421 ttacgctggc gcgcagaaaa atatcggccc ggcaggcctg acaatcgtca tcgtccgtga
     3481 agatttgctg ggcaaagcga atgtcgcgtg tccgtcgatt ctggattatt ccatcctcaa
     3541 cgataatggc tccatgttta acacgccgcc gacatttgcc tggtatctgt ctggtctggt
     3601 ctttaaatgg ctgaaagcga acggcggtgt agctgaaatg gataaaatca atcagcaaaa
     3661 agcagaactg ctatatgggg tgattgataa cagcgatttc taccgcaatg acgtggcgaa
     3721 agctaaccgt tcgcggatga acgtgccgtt ccagttggcg gacagtgcgc ttgacaaatt
     3781 gttccttgaa gagtcttttg ctgcaggcct tcatgcactg aaaggtcacc gtgtggtcgg
     3841 cggaatgcgc gcgtctattt ataatgccat gccgctggaa ggcgttaaag cgctgacaga
     3901 cttcatggtt gagttcgaac gccgtcacgg ttaatgccga aattttgctt aatccccaca
     3961 gccagcctgt ggggttttta tttctgttgt agagagttga gttcatggaa tccctgacgt
     4021 tacaacccat cgctcgtgtc gatggcacta ttaatctgcc cggttccaag agcgtttcta
     4081 accgcgcttt attgctggcg gcattagcac acggcaaaac agtattaacc aatctgctgg
     4141 atagcgatga cgtgcgccat atgctgaatg cattagcagc gttaggggta agctatacgc
     4201 tttcagccga tcgtacgcgt tgcgaaatta tcggtaacgg cggtccatta cacgcagaag
     4261 gtgccctgga gttgttcctc ggaaatgccg gaacggcaat gcgcccgctg gcggcagctc
     4321 tttgtctggg tagcaatgat attgtgctga ccggtgagcc gcgtatgaaa gagcgcccga
     4381 ttggtcatct ggtggatgcg ctgcgcctgg gcggggcgaa gatcacttac ctggaacaag
     4441 aaaattatcc gccgttgcgt ttacagggcg gctttactgg cggcaacgtt gacgttgatg
     4501 gctccgtttc cagccaattc ctcaccgcac tgttaatgac tgcgcctctt gcgccggaag
     4561 atacggtgat tcgtattaaa ggcgatctgg tttctaaacc ttatatcgac atcacactca
     4621 atctgatgaa gacgtttggt gttgaaattg aaaatcagca ctatcaacaa tttgtcgtaa
     4681 aaggcgggca gtcttatcag tctccgggta cttatttggt cgaaggcgat gcatcttcgg
     4741 cttcttactt tctggcagca gcagcaatca aaggcggcac tgtaaaagtg accggtattg
     4801 gacgtaacag tatgcagggt gatattcgct ttgctgatgt gctggaaaaa atgggcgcga
     4861 ccatttgctg gggcgatgat tatatttcct gtacgcgtgg tgaactgaac gctattgata
     4921 tggatatgaa ccatattccc gatgcggcga tgaccattgc caccgcggcg ttatttgcaa
     4981 aaggcaccac cacgctgcgc aatatctata actggcgtgt taaagagacc gatcgcctgt
     5041 ttgcgatggc aacagaactg cgtaaagtcg gtgcggaagt agaagagggg cacgattaca
     5101 ttcgtatcac tccaccggaa aaactgaact ttgccgagat cgcgacatac aatgatcacc
     5161 ggatggcgat gtgtttttcg ctggtggcgt tgtcagatac accagtgacg attcttgatc
     5221 ccaaatgtac ggccaaaaca tttccggatt atttcgagca gctggcgcgg attagccagg
     5281 cagcctgaat gagcaacggg caataaatag ccaaatcttt ctttatcaaa acgccggcac
     5341 attgccggcg ttttttttcg gaccttgtga gtcattttga ttaatggtag cgtcgcttgt
     5401 caatgtaagt tgttgataca taatatttat atatgattaa tcaacggatg attcacatga
     5461 agaatactaa attactgctg gcgattgcga cctctgcagc attactgaca gggtgtcaaa
     5521 atacccacgg tattgatacc aatatggcta tcagctccgg tttaaatgcc tataaagcag
     5581 caacgttaag cgatgccgat gcaaaagcga ttgccaatca gggctgtgcc gaaatggaca
     5641 gcggcaatca agtcgcaagt aaatccagca agtacggtaa acgtctggca aaaatcgcca
     5701 aagcattggg taacaatatt aatggcacgc cggtcaacta taaggtttat atgaccagcg
     5761 acgtcaacgc atgggcgatg gcgaacggct gtgttcgtgt ttacagtggc ctgatggaca
     5821 tgatgaacga taacgaaatt gaaggcgttc tgggccatga actgggccac gtcgcgttgg
     5881 gtcactcgct ggctgaaatg aaagcttctt atgcgatcgt tgccgcacgc gatgccattt
     5941 cagctaccag cggtgtggct tcccagcttt cccgctcaca attaggcgat atcgcagaag
     6001 gcgctatcaa tgctaaatac tcccgtgata aagagtccga agcagatgat ttctcctttg
     6061 atctgttgaa gaaacgtggc atcagcaccc aggggctggt tggcagcttt gaaaaactgg
     6121 ctagcctgga tggcggtcgc acccagtcca tgtttgactc tcacccacca tcaacagagc
     6181 gtgcgcaaca catccgtgat cgtatcgcct ctggtaagta aatcattgtc atctttcggg
     6241 ctggtcttct gccagcccgc tataattgcg caataaatcc ccatctgaat acagacaaaa
     6301 ctggtttttg cacacaacgt taacgatttg tggcgtcggc gcgtataatg cgcgcggtta
     6361 tgttaacggt acgcctgttt taaggagata aagatgacgg caattgcccc ggttattacc
     6421 attgatggcc caagcggtgc agggaaaggc accttgtgta aggctatggc ggaagcgttg
     6481 caatggcatc tgctggactc gggtgcaatt tatcgcgtac tggcactagc ggcattacat
     6541 caccatgttg atgttgcttc ggaagatgcg ctggtgccgc tggcatccca tctggatgta
     6601 cgttttgtgt cgaccaatgg caatctggaa gtgatcctcg aaggggaaga tgtcagcggc
     6661 gaaattcgta ctcaggaagt ggcgaatgct gcttcacaag tcgcggcatt cccacgcgtt
     6721 cgtgaagcat tattgcgtcg ccaacgcgcg tttcgcgaat taccaggcct gattgccgat
     6781 ggccgcgaca tgggaacggt ggtattccct gatgcgccag tgaaaatttt ccttgacgcc
     6841 tcctcggaag aacgtgcgca tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt
     6901 aactttgaac gccttttggc cgagatcaaa gaacgcgacg accgcgatcg taaccgagcg
     6961 gtagcgccac tggttccggc agccgatgct ttagtgttgg attccaccac cttaagcatt
     7021 gagcaagtga ttgaaaaaac gctacaatac gcgcgccaga aattggctct cgcataagcg
     7081 accgaatttg cagtaccccc gttgcaatgg aatgacagcg ggtatgttaa acaaccccat
     7141 ccggcatgga gccaggtgga cgttaaatat aaacctgaag attaaacatg actgaatctt
     7201 ttgctcaact ctttgaagag tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc
     7261 gtggcgttgt tgttgctatc gacaaagatg tagtactggt tgacgctggt ctgaaatctg
     7321 agtccgccat cccggctgag cagttcaaaa acgcccaggg cgagctggaa atccaggtag
     7381 gtgacgaagt tgacgttgct ctggacgcag tagaagacgg cttcggtgaa actctgctgt
     7441 cccgtgagaa agctaaacgt cacgaagcct ggatcacgct ggaaaaagct tacgaagatg
     7501 ctgaaactgt taccggtgtt atcaacggca aagttaaggg cggcttcact gttgagctga
     7561 acggtattcg tgcgttcctg ccaggttctc tggtagacgt tcgtccggtg cgtgacactc
     7621 tgcacctgga aggcaaagag cttgaattta aagtaatcaa gctggatcag aagcgcaaca
     7681 acgttgttgt ttctcgtcgt gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc
     7741 tgctggaaaa cctgcaggaa ggcatggaag ttaaaggtat cgttaagaac ctcactgact
     7801 acggtgcatt cgttgatctg ggcggcgttg acggcctgct gcacatcact gacatggcct
     7861 ggaaacgcgt taagcatccg agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag
     7921 tgctgaagtt cgaccgcgaa cgtacccgtg tatccctggg cctgaaacag ctgggcgaag
     7981 atccgtgggt agctatcgct aaacgttatc cggaaggtac caaactgact ggtcgcgtga
     8041 ccaacctgac cgactacggc tgcttcgttg aaatcgaaga aggcgttgaa ggcctggtac
     8101 acgtttccga aatggattgg accaacaaaa acatccaccc gtccaaagtt gttaacgttg
     8161 gcgatgtagt ggaagttatg gttctggata tcgacgaaga acgtcgtcgt atctccctgg
     8221 gtctgaaaca gtgcaaagct aacccgtggc agcagttcgc ggaaacccac aataagggcg
     8281 accgtgttga aggtaaaatc aagtctatca ctgacttcgg tatcttcatc ggcctggacg
     8341 gcggcatcga cggcctggtt cacctgtctg acatctcctg gaacgttgca ggcgaagaag
     8401 cagttcgtga atacaaaaaa ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag
     8461 aacgtgaacg tatctccctg ggcgttaaac agctcgcaga agatccgttc aacaactggg
     8521 ttgctctgaa caagaaaggc gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag
     8581 gcgcaaccgt agaactggct gacggcgttg aaggttacct gcgtgcttct gaagcatccc
     8641 gtgaccgcgt tgaagacgct accctggttc tgagcgttgg cgacgaagtt gaagctaaat
     8701 tcaccggcgt tgatcgtaaa aaccgtgcaa tcagcctgtc tgttcgtgcg aaagacgaag
     8761 ctgacgagaa agatgcaatc gcaactgtta acaaacagga agatgcaaac ttctccaaca
     8821 acgcaatggc tgaagctttc aaagcagcta aaggcgagta attctctgac tcttcgggat
     8881 ttttattccg aagtttgttg agtttacttg acagattgca ggtttcgtcc tgtaatcaag
     8941 cactaagggc ggctacggcc gcccttaatc aatgcagcaa cagcagccgc ttaatttgcc
     9001 tttaaggaac cggaggaatc atgaccaagt cagaattgat agaaagactt gccacccagc
     9061 aatcgcacat tcccgccaag acggttgaag atgcagtaaa agagatgctg gagcatatgg
     9121 cctcgactct tgcgcagggc gagcgtattg aaatccgcgg tttcggcagt ttctctttgc
     9181 actaccgcgc accacgtacc ggacgtaatc cgaagactgg cgataaagtt gaactggaag
     9241 gaaaatacgt tcctcacttt aaaccaggta aagaactgcg cgatcgcgcc aatatttacg
     9301 gttaagtttt tactcaaact tgaacgcgag aaaagcacct gtcgggtgct tttttcattt
     9361 ctctaatcag gaactggaag ctgcctcgca gagttttgag cagttttcac cctttcgtta
     9421 aattcttctg aatgtgcctc ggggaacgca aaattcccac cagacaaccg ctcaacaaag
     9481 ttgcacactt tccataaaca gggaggggtg catgaaaata acgacagtcg gcgtatgcat
     9541 aatttgcgga atttttccgt tgctgatttt gccccaattg cctgggacat taacccttgc
     9601 gtttctgact ctcttcgcct gcgtactggc atttatccct gttaaaaccg tccgttatat
     9661 cgcgctgacg ttgctgtttt tcgtttgggg catattatca gcaaagcaaa ttttgttggc
     9721 aggagaaacc ttaactggcg cgacgcagga tgcaattgtt gagatcactg ctactgacgg
     9781 catgaccact cattacggtc aaattactta tctacaaggt cgacgtatat tccctgcgcc
     9841 aggccttgtg ctgtatggcg aatatcttcc gcaagcggtt tgtgccggac aactgtggtc
     9901 aatgaaactc aaagttcgtg ccgttcatgg tcaacttaat gatggcggct ttgatagcca
     9961 gcgttatgcc attgcccagc atcagccgct caccggccgc tttctgcagg caagtgttat
    10021 tgaaccgaat tgtagcctgc gtgcacagta tctggcgtca ctacaaacaa cgctgcaacc
    10081 ctatccgtgg aatgcggtta ttcttggttt aggtatgggg gaacggttat ccgtccctaa
    10141 agaaatcaaa aatatcatgc gtgatactgg aacggcgcat ttaatggcga tatcgggatt
    10201 gcacatcgct tttgcggcgt tgctggctgc cggactcatt cgcagtggac aaatttttct
    10261 gcctgggcgc tggatccact ggcaaatgcc attaattggc ggaatctgct gtgctgcttt
    10321 ttatgcctgg ctgacgggaa tgcaacctcc tgcattgcgt accgttgtgg cgcttgctac
    10381 gtggggaatg cttaagttaa gtgggcgaca gtggagtggc tgggatgtat ggatatgttg
    10441 tctggcggca attttgctga tggatcctgt tgccattctc tcgcaaagtt tatggctctc
    10501 tgccgctgcg gtcgcggcat tgatattttg gtatcagtgg tttccctgtc ctgagtggca
    10561 actgccgccg gtattgcgtg cagttgtttc cctcattcat ttgcaactgg gaatcacact
    10621 tctgcttatg cccgtgcaaa tcgtcatatt tcatggcatt agtctgacct cgtttattgc
    10681 aaatctatta gcaattccct tggtgacatt tatcacggtt ccgttgatcc tcgccgcgat
    10741 ggttgtgcat ttaagcgggc cgttaatcct ggagcaaggg ttatggtttc ttgccgaccg
    10801 gtctttggct ttacttttct gggggttaaa gagtttgcca gaagggtgga tcaacattgc
    10861 tgaacgttgg caatggctat cattttcccc atggttctta ctggtggtat ggcgattaaa
    10921 cgcctggcga acgttgccag caatgtgtgt ggctgtaggc ttgctgatgt gctggccgct
    10981 gtggcaaaaa cctcgacctg acgagtggca agtgtacatg cttgatgtcg ggcaagggct
    11041 ggcaatggtg atagccagaa acggcaaagc gattctctat gacacgggac tggcctggcc
    11101 cgaaggggat agtgggcaac aactgattat cccctggctc cactggcata atcttgaacc
    11161 ggaaggcgtt attctgagcc atgaacatct ggatcaccgg ggagggctgg actcaatatt
    11221 gcacacatgg ccgatgttat ggatcagaag tccgttaaac tgggaacatc atcagccctg
    11281 tgtgcgtggc gaagcgtggc aatggcaagg attgcgtttc agcgcgcact ggcctttaca
    11341 aggtagcaac gataaaggaa ataaccattc ctgtgtggtt aaggttgatg acgggacgaa
    11401 tagcattctt ctaaccggtg atattgaagc cccagctgaa caaaagatgc taagccgtta
    11461 ctggcagcaa gtgcaggcaa cattgcttca ggtacctcac catggcagta atacctcatc
    11521 atcgttgcca ttaattcagc gagtgaatgg aaaagtggca ctcgcatcgg catcgcgcta
    11581 taacgcatgg cgactgccct ctaacaaagt taagcatcgc tatcaacaac aaggttatac
    11641 gtggcttgat actcctcatc aggggcaagt aacggtcgat ttttcagcgc aaggctggcg
    11701 gattagcagc ctcagggagc aaattttacc tcgttggtat catcagtggt ttggcgtgcc
    11761 agtggataac gggtagaata tgcggctatt tcaacaaatg ctggtttttt gaatgcataa
    11821 cgacaaagat ctctctacgt ggcagacatt ccgccgactg tggccaacca ttgcgccttt
    11881 taaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt
    11941 catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt
    12001 gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttgcgtggta tcaccagcta
    12061 tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg
    12121 cctgtttggt cacatgatgg gaatgccggt ttcattcttt gacaaacagt caacgggtac
    12181 gctgttgtca cgtatcacct acgattccga acaggttgct tcttcctctt ccggcgcact
    12241 gattactgtt gtgcgtgaag gtgcgtcgat catcggcctg ttcatcatga tgttctatta
    12301 cagttggcaa ttgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg
    12361 cgtagtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt
    12421 gaccaccagc gctgaacaaa tgctgaaagg ccataaagaa gtattgattt tcggtggtca
    12481 ggaagtggaa acgaaacgct tcgataaagt cagcaaccga atgcgtcttc aggggatgaa
    12541 aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct
    12601 ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac
    12661 gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat cgctgaccaa
    12721 cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta ccattctgga
    12781 cagtgagcag gagaaagacg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga
    12841 attccgcaac gtcaccttta cttatccggg acgtgacgta cccgcattgc gtaacatcaa
    12901 cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt caggtaaatc
    12961 aaccatcgcc agcctgatca cgcgttttta cgatattgat gaaggcgaaa tcctgatgga
    13021 tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg ctctggtgtc
    13081 gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg cacggactga
    13141 acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat
    13201 caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc tgctctctgg
    13261 cggtcagcgt cagcgtattg ctatcgctcg agccttgttg cgtgatagcc cgattctgat
    13321 tctggacgaa gctacctcgg cgttggatac cgaatccgaa cgtgcgattc aggcggcact
    13381 ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga
    13441 aaaggcagac gaaatcgtgg tcgttgagga tggtgtcatt gtggaacgcg gtacgcataa
    13501 cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg
    13561 atcgaaaaaa tctggtctgg tgaatcccct ttgtggcggc tattgctgcc actctcctgg
    13621 ttgtatggcc tggtgagtgg cgcgatccgt ctttgctata aactaaaact gaagcgcgcc
    13681 tggcgtgccc ccgtaccggt tgtcgtggtt ggtaatctca ccgcaggcgg caacggaaaa
    13741 accccggtcg ttgtctggct ggtggaacag ttgcaacagc gcggtattcg cgtgggggtc
    13801 gtatcgcggg gatatggtgg taaggctgaa tcttatccgc tgttattgtc ggcagatacc
    13861 accacagcac aggcgggtga tgaacctgtg ttgatttatc aacgcactga tgcgcctgtt
    13921 gcggtttctc ccgttcgttc tgatgcggta aaagccattc tggcgcaaca ccctgatgtg
    13981 cagatcatcg taaccgacga cggtttacag cattaccgtc tggcgcgtga tgtggaaatt
    14041 gtcgttattg atggtgtgcg tcgctttggc aatggctggt ggttgccggc ggggccaatg
    14101 cgtgagcgag cggggcgctt aaagtcagtt gatgcggtaa tcgtcaacgg cggtgtcccc
    14161 cgcagcggtg aaatccccat gcatctgctg ccgggtcagg cggtgaattt acgtaccggt
    14221 acgcgttgtg acgttgctca gcttgaacat gtggtggcga tggcagggat tgggcatccg
    14281 ccgcgctttt ttgccacgct gaagatgtgc ggcgtacaac cggaaaaatg tgtaccgctg
    14341 gccgatcatc agtctttgaa ccatgcggat gtcagcgcgt tggtaagcac cgggcaaacg
    14401 ctggtaatga ctgaaaaaga tgcggtgaaa tgccgggcct ttgcagaaga aaattggtgg
    14461 tatttgcccg ttgacgcaca gctttcaggt gatgaaccag cgaaactgct tgcgcaacta
    14521 acctcgctgg cttctggcaa ctagttacgc cgcggcagcg ttcgattgat ggagtcatga
    14581 atgtcgctgc cgcacctctc ccttgctgat gcgcgtaatc ttcaccttgc cgcgcaaggc
    14641 ctgttaaaca aaccccgccg tcgagcgtcg ttggaggata ttccggcaac gatctcccgc
    14701 atgtccttgc tgcaaatcga taccattaat attgttgccc gtagtccata tctggtgctt
    14761 ttcagtcgtc tgggaaatta tcctgcccag tggctggatg agtctctggc gcgtggcgaa
    14821 ttaatggaat actgggcgca tgaagcctgc tttatgccac gcagcgactt tcgtcttatt
    14881 cgccaccgca tgctggcacc tgaaaaaatg ggctggaaat acaaagacgc ctggatgcag
    14941 gaacatgagg cggaaattgc acagttaatt cagcatattc atgataaggg gccggtacgt
    15001 tcagccgatt ttgagcatcc tcgtaaaggt gcaagcggct ggtgggaatg gaagccgcat
    15061 aaacggcatc tggaaggttt atttactgcc ggaaaggtga tggtgattga acggcgcaac
    15121 ttccagcgcg tttatgattt aacccaccgt gtcatgcctg actgggatga tgagcgcgat
    15181 ctcgtttcgc aaacagaagc agaaatcatc atgctggata acagtgcgcg tagcctggga
    15241 atattccgcg aacagtggct ggcagattac tatcggctga aacgtccggc actggcagcg
    15301 tggcgcgaag cgagggctga acagcggcaa atcattgctg tgcatgttga aaaattgggc
    15361 aatctttggc tgcatgctga tttgctgccg ctactcgagc gagcgctggc cggaaagctc
    15421 actgcaacgc acagcgcggt actttcgcct tttgatcctg ttgtctggga tcgcaaacgc
    15481 gcagagcagc tttttgattt tagctaccgg ctggagtgct ataccccagc gccgaaacgc
    15541 cagtatggct attttgttct gccgttatta catcgtgggc aattagttgg gcgaatggat
    15601 gccaaaatgc atcgccagac aggcatcctt gaagttatct ctctgtggtt acaggaaggt
    15661 attaaaccaa cgacaacgct gcaaaaaggg ttacgtcagg cgattactga tttcgctaac
    15721 tggcagcagg caacgcgggt gacattagga cactgcccgc aaggtctctt tacggattgc
    15781 cgcaccggct gggaaataga ccccgtcgca taaatgatta tgataagatt tatgcattca
    15841 taacccgatc catctggagg aactatggat catcgtctgc ttgaaatcat tgcctgcccg
    15901 gtttgcaacg gaaaactttg gtataaccag gaaaaacaag agcttatttg caaactggac
    15961 aacctggctt tcccgctgcg cgatggcatt ccagtgttgc tggaaacgga agcccgcgtg
    16021 ctgactgctg atgagagtaa atcatgagtt ttgtggtcat tattcccgcg cgctacgcgt
    16081 caacgcgtct gcccggtaaa ccattggttg atattaatgg caaacccatg attgttcatg
    16141 ttcttgaacg tgctcgcgaa tcaggtgccg agcgcatcat cgtggcaacc gatcatgagg
    16201 atgtcgcccg cgccgttgaa gccgctggcg gtgaagtatg tatgacgcgc gccgatcacc
    16261 agtcagggac agaacgcttg gcggaagttg tcgaaaaatg cgcgttcagc gacgacacgg
    16321 tgatcgttaa tgtgcagggt gatgaaccga tgatccctgc gacaatcatt cgtcaggttg
    16381 ctgataacct cgctcagcgt caggtgggta tggcgactct ggcggtgcca atccacaatg
    16441 cggaagaagc gtttaacccg aatgcggtga aagtcgttct cgacgctgaa gggtatgcac
    16501 tgtacttctc acgcgcaacc attccgtggg atcgtgatcg ttttgcaaaa ggccttgaaa
    16561 ccgttggcga taacttcctg cgtcatcttg gtatttatgg ctaccgtgca ggctttatcc
    16621 gtcgttacgt cacctggcag ccaagcccgt tagaacacat cgaaatgtta gagcagcttc
    16681 gtgttctgtg gtacggcgaa aaaatccatg ttgctgtcgc gcatgaagtt cctggcacag
    16741 gtgtggatac ccctgaagat ctcgagcgcg ttcgcgctga aatgcgctaa tttcacttca
    16801 cgacactcca gccaattttg ggaggagtgt cgtaccgtta cgattttcct caatttttct
    16861 tttcaacaat tgatctcatt caggtgacat cttttatatt ggcgctcatt atgaaagcag
    16921 tagcttttat gagggtaatc tgaatggaac agctgcgtgc cgaattaagc catttactgg
    16981 gcgaaaaact cagtcgtatt gagtgcgtca atgaaaaagc ggatacggcg ttgtgggctt
    17041 tgtatgacag ccagggaaac ccaatgccgt taatggcaag aagctttagt acgcccggaa
    17101 aagcccgaca actggcatgg aaaaccacca tgctggcaag aagtgggact gtccgtatgc
    17161 cgactattta tggtgtgatg acgcatgaag aacaccccgg ccccgatgtc ctgctactgg
    17221 agcggatgcg tggtgtttcg gtggaagcac cagcccgaac accagaacgc tgggaacaac
    17281 tcaaagacca aatcgttgaa gccttactgg cctggcaccg tcaggacagt cgcggttgcg
    17341 tcggcgcggt cgacaatact caggaaaatt tctggccctc atggtaccgg caacatgttg
    17401 aagtgctatg gaccacgctc aatcagttca ataacaccgg tctaacgatg caggataagc
    17461 ggatcctgtt ccgcactcgc gaatgtctcc cggcattatt tgaaggcttt aacgacaatt
    17521 gtgtgctgat tcacggtaac ttctgtttac gcagcatgct gaaagattcg cgcagcgatc
    17581 agttactggc gatggtcggg ccgggactaa tgctttgggc accgcgagaa tacgaactgt
    17641 tccgactaat ggataattct ctggcggaag atttgctctg gagttacctg caacgcgcgc
    17701 cagtggcgga gtcgttcatc tggcggcgtt ggttgtatgt gttatgggat gaagttgcgc
    17761 aactggttaa taccggacga tttagtcggc gcaacttcga tctggcatca aaatcactct
    17821 tgccgtggct cgcctgacgg acctttcagc cactgccaga tgcggccaag cgtttcatag
    17881 ccaacacgat cgctatgcat cagccagacc ggagaaggga ttgcccgttc ccacggatta
    17941 agcggcgagt cgatggccaa ctgattcgcc ggagctggta gcggatttaa cccttcctgc
    18001 tgaaaaaaga tcatcgcccg cggcagatgt gatgcggagg tcaccagcag gaaaggggcg
    18061 tcaccaatcg cctgtttcac tgccgcagct tcttcttcgg tatcttttgg caaatccagg
    18121 gtgataattt gctcgcgcgg tacgcccagc gattgcgcaa ctctggcacc tacttccgct
    18181 gtactcaccg tattggtttt tgctacgcct cccgtgaaga tcagttttga acccggattt
    18241 tcgcgccata aacggatacc ctcattcagg cgtggcagac tgttattgat taaattagag
    18301 ctcggtgccc actgcgggtt ccaggtataa ccacctccga gcaccacgat atagtccact
    18361 ttttgcgaat tattccatgt cggataggtg ctttcgatgg gacgcagcag gcggtcggct
    18421 accggttgca aactcaataa caagagtgcc agccacccaa tgctaataaa aatcttcccg
    18481 gttttttgaa aacggctaaa ccacagcagc gccaggccag cgccaataat taacaacatc
    18541 agcggaagtg gcagcaacat attgccaatc acttttttca gtgtaaaaag catcctttct
    18601 ggttcctttt ttaaccatat agcaagggat cgccagcgat attacaccag acgggttcat
    18661 tctcgccgtg gctgtgacaa aatagcgtct ttatcagaaa acgtcttagc caaccggtgg
    18721 agaaagcaat gcaggatcgc aattttgatg atattgcgga aaagttttcc cgtaacattt
    18781 acggcaccac caaagggcag cttcgacagg ctattctgtg gcaggatctc gatcgcgtgc
    18841 tggcggaaat gggtccgcaa aaactgcgtg tgctggatgc gggcggtgga gaagggcaga
    18901 ccgcaatcaa aatggccgag cgcgggcatc aggtcatttt atgcgatctt tctgcgcaga
    18961 tgatcgaccg cgcaaaacag gcggcagaag caaaaggtgt gagcgacaac atgcaattta
    19021 tacattgcgc cgctcaggat gttgcttcgc atttggaaac gcccgttgat ctgatattgt
    19081 tccatgcggt gctcgagtgg gtggctgatc cccgcagcgt attgcagacc ctgtggtcag
    19141 tgttgcgtcc aggcggcgtg ttgtcgttaa tgttctacaa tgcgcatggt ttgttgatgc
    19201 ataacatggt cgccgggaat tttgattacg tgcaggcggg aatgccgaaa aagaaaaaac
    19261 ggacgctttc gccagattat ccacgcgacc cggcgcaggt ttatctgtgg ctggaagaag
    19321 ctggttggca aattatgggt aagacaggcg ttcgcgtgtt tcatgattat ctgcgcgaga
    19381 agcaccagca gcgcgactgc tatgaagcat tacttgaatt agaaacgcgt tattgccgtc
    19441 aggaaccgta tattaccctg gggcgttata ttcatgtcac cgcgcgcaaa ccgcagagca
    19501 aggataaagt atgagtgaat tttcccagac agtccccgaa ctggttgcct gggccagaaa
    19561 aaatgacttc tccatctcgc tgccggtaga ccgactctct tttctgctgg cggttgccac
    19621 gctgaacggc gagcgtctgg atggtgagat gagtgaaggc gagctggtgg atgcattccg
    19681 ccatgtgagt gatgcgtttg agcaaaccag cgaaaccatc ggcgtgcgcg ccaataacgc
    19741 gatcaacgac atggtgcgtc aacgtctgct gaaccgcttt accagcgagc aggcagaagg
    19801 gaacgcaatt taccgtctga cgccgctcgg catcggcatt actgactatt acatccgtca
    19861 gcgcgagttt tctacgctgc gcctttctat gcagttgtcg attgtggcgg gtgagctcaa
    19921 acgcgcagca gatgccgccg aagagggcgg tgatgaattt cactggcacc gtaatgtcta
    19981 tgcgccactg aaatattcgg tagcagaaat tttcgacagt atcgacctga cgcaacgtct
    20041 gatggacgaa cagcagcagc aggtgaagga cgatatcgcc cagttgctga acaaagactg
    20101 gcgggcggcg atttccagct gtgaattgtt gctttcggaa acttccggaa cgctgcgtga
    20161 attgcaggat acgctggaag cggcaggcga caaattgcag gctaatctgt tgcgcattca
    20221 ggatgcgacg atgactcatg acgatctgca ttttgtcgat cgtctggtgt tcgatctgca
    20281 gagcaaactc gaccgtatta tcagttgggg ccagcaatcc atcgatttgt ggattggcta
    20341 cgaccgccac gtacataaat ttattcgtac cgcgatcgat atggataaaa accgcgtctt
    20401 tgctcagcgg ttacgtcagt cggtacaaac ctattttgat gagccgtggg cgctaactta
    20461 tgccaatgcc gatcgtctgc tggatatgcg tgacgaagag atggcactgc gcgatgaaga
    20521 agtgactggg gaacttcctg aggatctgga atacgaagag tttaacgaga tccgcgaaca
    20581 gctggcggcg atcatcgaag aacaacttgc tgtgtacaaa accagacaag tgccgctgga
    20641 tcttggtctg gtggtacgcg aatatctgtc acagtatccg cgtgcacgtc actttgacgt
    20701 tgcgcgcatt gttattgatc aggcggtacg tcttggcgta gcgcaagcag atttcaccgg
    20761 actgccagcg aaatggcagc cgattaatga ttacggagcc aaggtacagg cgcatgtcat
    20821 cgacaaatat tgaacaagtg atgccggtta agctggcgca ggcgctggcg aatccgttat
    20881 ttccggcgct ggacagcgcc ttacgttcag gccgccatat tggcctcgac gaactggata
    20941 atcatgcatt cctgatggat tttcaggaat atctggaaga gttttacgcg cgttataacg
    21001 ttgagcttat tcgtgcaccg gaagggttct tctatttacg tccacgttcc accacgctga
    21061 tccctcgttc cgtcttgtcg gaactggata tgatggtcgg gaaaatcctc tgttatctct
    21121 atctcagccc ggaacggctg gcgaatgagg ggatttttac tcaacaggaa ctgtacgacg
    21181 aactgctcac cctggctgat gaagcgaaac tgctgaaact ggtgaacaac cgttcaaccg
    21241 gttcagacgt tgaccgtcag aagttgcagg aaaaagtacg ttcttcgctc aaccgtctgc
    21301 gtcgtttagg catggtgtgg tttatgggcc acgacagcag caagttccgc attaccgaat
    21361 cggtgttccg cttcggagcc gatgtgcgtg ctggcgacga tccccgtgaa gcacagcgtc
    21421 gcctgattcg tgatggcgaa gcaatgccga ttgaaaatca tctgcaactc aacgatgaaa
    21481 ccgaagagaa tcagccagat agcggagagg aagaataatg attgaacgcg gtaaatttcg
    21541 ctcactgacg ctgattaact ggaacggctt ttttgcccga acttttgacc ttgacgagct
    21601 ggtcacgacg ctttctggcg gtaacggggc gggtaaatcc accaccatgg cggcgttcgt
    21661 tacggcgctg atccccgacc tgaccctgct gcatttccgt aacactacgg aagccggggc
    21721 caccagcggt tcgcgcgata aaggtctgca cggtaagctg aaagcgggtg tctgttattc
    21781 gatgctcgac accattaact cgcgccacca gcgcgtggtg gtcggtgtgc gtctgcaaca
    21841 ggttgccgga cgcgatcgta aagtggatat caaaccattt gccattcagg gactgccgat
    21901 gtcggtgcag ccgacacagc tggtgaccga aactctgaac gaacgccagg cgcgcgtgct
    21961 gccgcttaac gagctgaaag acaagctcga ggcgatggaa ggcgtgcagt ttaaacagtt
    22021 caactccatt accgattacc actcgctgat gttcgatctg ggcatcatcg cgcgtcgtct
    22081 gcgctccgca tctgaccgta gcaaattcta tcgtctgatc gaagcttcgc tgtatggcgg
    22141 gatctccagt gccattaccc gttctctgcg cgactacctg ttgccggaaa acagcggcgt
    22201 gcgtaaagcg ttccaggaca tggaagcggc gctgcgtgaa aaccgcatga cgctggaagc
    22261 gattcgtgtc acccaatcgg accgtgacct gtttaagcat ctgatcagcg aagctaccaa
    22321 ctacgtggcg gcggactaca tgcgtcacgc caacgagcgc cgtgtccatc tcgacaaagc
    22381 gctggagttt cgtcgcgagc tacatacttc gcgtcagcaa ctggcggctg agcagtacaa
    22441 acacgtcgat atggcgcgtg agctggcaga gcacaacggt gccgaaggcg atctggaagc
    22501 ggattatcag gcggccagcg atcacctgaa cctggtgcaa accgcactgc gtcagcagga
    22561 gaagatcgaa cgctacgaag cggatctcga tgagctgcag atccgtctgg aagagcaaaa
    22621 cgaagtggtg gcagaagcca tcgaacgcca ggaagagaac gaggctcgtg cggaagctgc
    22681 cgaactggaa gtggacgagc tgaaaagcca gcttgctgac taccagcagg cgctggacgt
    22741 ccagcaaacg cgcgcgatcc agtataacca ggcgattgct gcgcttaatc gtgccaaaga
    22801 actgtgccat ctgccggact taaccgccga cagcgccgcc gaatggctgg aaaccttcca
    22861 ggcgaaagag ctggaagcga ctgaaaaaat gctctctctt gagcagaaaa tgagcatggc
    22921 gcaaaccgcg cacagccagt ttgagcaggc ttatcagctg gtggtggcaa tcaacggccc
    22981 gctggcgcgt aacgaggcgt gggatgtcgc tcgcgaacta ttgcgcgaag gggtcgatca
    23041 gcgtcacctg gcagagcagg ttcagccgct gcggatgcga ttaagcgaac tggaacagcg
    23101 tctacgcgag cagcaagaag ctgagcgtct gctggcagat ttctgcaaac gtcagggcaa
    23161 gaattttgat attgatgaac tggaagctct gcatcaggag ctggaagcac gcattgcctc
    23221 tctttccgat agcgtatcta acgcccgtga agagcggatg gcactgcggc aggaacagga
    23281 acagctgcag tctcgcattc agagtttgat gcagcgtgcg ccggtttggc tggcagcgca
    23341 aaacagtctc aaccagttga gcgaacagtg cggcgaagag ttcacctcca gccaggacgt
    23401 caccgaattc ctgcaacagc tgctggagcg tgagcgcgaa gccattgttg aacgcgatga
    23461 agtgggcgcg cgcaaaaacg ctgtcgatga agagatcgaa cgtttaagcc agcctggcgg
    23521 ctctgaagac cagcgtctga atgcgctggc ggagcgtttt ggcggcgtgc tgctgtcaga
    23581 aatttatgat gacgttagcc tggaagatgc gccgtacttc tcagcgctgt atggcccgtc
    23641 acgccacgcc atcgtggtgc cagatctgtc acaggtaact gaacacctgg agggcttgac
    23701 cgattgcccg gaagatctct atctgatcga aggggatccg cagtcattcg atgacagcgt
    23761 gttcagcgtt gatgagctgg aaaaagcggt agtggtgaaa atcgccgatc gtcagtggcg
    23821 ttattcacgt ttcccggaag tgccgctgtt tggtcgtgct gcgcgtgaaa gccgtattga
    23881 aagcctccat gccgagcgtg aagtgctttc cgaacgcttc gccacgctct cctttgatgt
    23941 acagaaaact cagcgtctgc atcaggcgtt cagccgcttt atcggcagtc atctggcggt
    24001 ggcgtttgag tctgacccgg aagcagaaat ccgtcaactg aacagccgtc gcgtcgaact
    24061 ggagcgggcg ttgagtaatc atgaaaatga taaccagcag cagcgtattc agtttgagca
    24121 ggcgaaagag ggcgttacgg cgctgaaccg cattctgccg cgtctcaacc tgttggctga
    24181 tgacagcctg gcggatcgcg tcgatgaaat ccgcgaacgt ctggatgaag cccaggaagc
    24241 cgcgcgtttt gttcagcagt ttggcaatca actggcgaaa ctggaaccga tcgtttcggt
    24301 attgcagagc gacccggaac agttcgaaca gttaaaagaa gattacgcgt actctcagca
    24361 gatgcagcgc gatgcccgtc agcaggcgtt tgccctgacg gaagtggtgc agcgtcgtgc
    24421 gcactttagc tattctgact cggcagaaat gcttagcggt aacagcgatc tcaacgaaaa
    24481 attgcgtgaa cgtctggaac aggcggaagc ggagcgtacc cgcgctcgtg aagcgttgcg
    24541 cggtcacgca gcgcagttga gtcagtacaa ccaggtgctg gcttcgctga aaagttcgta
    24601 tgacaccaaa aaagagctac ttaacgatct gcaacgtgaa ttgcaggata tcggcgtgcg
    24661 tgctgatagc ggggcagaag agcgggcacg tattcgccgt gacgagctgc atgcgcaact
    24721 gagcaataac cgttcgcgcc gcaaccaact ggaaaaagcg ctcaccttct gcgaagcgga
    24781 gatggacaac ctgacccgca aactgcgcaa gctggagcgt gattactttg agatgcgcga
    24841 gcaggtagtg accgcgaaag cgggctggtg tgcagtcatg cgcatggtga aagataacgg
    24901 cgttgagcgc cgcttacacc gtcgtgagct ggcttatctc tccgctgatg atttgcgttc
    24961 catgtcggat aaggcgttag gtgcgctgcg tctggcggtg gcggataacg aacatctgcg
    25021 cgacgtgctg cgcatgtcgg aagatccgaa acgtccggag cgtaaaattc agttcttcgt
    25081 ggtggtttat cagcatctgc gtgaacgtat tcgtcaggat attattcgta ccgatgatcc
    25141 ggtggaagct atcgaacaga tggagatcga acttagccgt ctgaccgaag aattaacctc
    25201 ccgtgaacag aaactggcga tcagttcccg cagcgtggcg aacatcattc gcaaaaccat
    25261 tcagcgcgag cagaaccgta tccgtatgct caaccagggg ttgcagaacg tatcgtttgg
    25321 tcaggtgaac agcgtgcgcc tcaacgtgaa cgtgcgtgaa acgcacgcca tgctgctgga
    25381 tgtgctctct gaacagcatg agcagcatca ggatctgttt aacagcaacc gtctgacctt
    25441 ctcggaagcg ctggcgaaat tgtatcagcg tcttaatccg cagattgata tggggcagcg
    25501 cacgccgcag accattggcg aagaactgct ggattaccgc aactatctgg aaatggaagt
    25561 tgaggttaac cgtggttccg atggctggtt gcgcgcagag tctggtgcat tgtcgaccgg
    25621 tgaggcgatt ggtaccggta tgtcgattct ggtgatggtg gtacaaagct gggaagatga
    25681 atctcgccgc ctgcgcggta aagatatctc tccttgccgc ctgctgttcc tcgatgaagc
    25741 agcgcgactg gatgctcgtt ctatcgccac gctgtttgaa ttgtgtgagc gtttgcaaat
    25801 gcagctcatc atcgcagcgc cggaaaatat cagcccggag aaaggcacca cctataaact
    25861 ggtgcgtaaa gtcttccaga ataccgaaca tgttcatgtc gtcggcctgc gaggatttgc
    25921 gccgcaactc cctgaaacgc ttccaggaag tgacgaagcg ccttctcagg cgagttaaaa
    25981 ttaaggcggc agcaatgccg ccttttcttt ttccgaaaac tccgtttctg cactaaaaaa
    26041 gtggcacatt acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta
    26101 tcggcgttta tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg
    26161 tgagtattgg cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat
    26221 aaaaacaggg ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc
    26281 agtttgtgcc tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa
    26341 gtaatccctg gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg
    26401 caagccacgg caataatggc ggggatccag ccattgcctg aaggtgcggc agaaaaagcc
    26461 cgcacgcaaa tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt
    26521 caactgttgt atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa
    26581 gccttccagc aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa
    26641 tgggtagagt tactgaccga tcctggtgtt aacgggatgg cacgcgacgt ggtgctctct
    26701 gatgcgatga tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg
    26761 ctatatagca gtaaacctta tgcgcttgca acgccgccgc tttcggtgat taaccaatgg
    26821 cagcaggcgc tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat
    26881 ccgcaatatg cggcgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg
    26941 ccccaactga ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg
    27001 ttgcgtgaaa tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct
    27061 ggcgatgaca cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca
    27121 gaaactaagc cgatggacaa gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgt
    27181 gccgcctacg ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg
    27241 ggggcagatg gtgctattgg cccggcaacg cgggactggt taaacgtaac gcccgcccag
    27301 cgtgctggcg tgttggcact caacatccag cgattgcgct tgctgccaac agagctttct
    27361 accgggatca tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag
    27421 gtgctggatt cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt
    27481 gcccttaaca acgtagtggt aaacccgccg tggaacgtac caccaactct ggcacgcaaa
    27541 gatattctgc cgaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg
    27601 atgcgcggct ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc
    27661 acggcctcga atttaccgtt tcgcttccag caggctccag gcccacggaa ctcgctgggg
    27721 cgctataaat tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac
    27781 aatctgttca agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct
    27841 tccgatctgg cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat
    27901 gcgctgaagc agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc
    27961 tactacctga cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac
    28021 aattatgatc tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc
    28081 aggtaaatga agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggtga
    28141 ttctctgcag cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg
    28201 ttaaggtgcc tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg
    28261 attatcatgg acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca
    28321 ctcggtgccg ccatcctgcc gacccctgcg tttgcaacac tctctacccc acgcccgcgc
    28381 attttgacac tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc
    28441 agaggctata ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg
    28501 aacaaaataa agtccatcga cctaggatta ttcgaccagt tgtatcgcct gcaagggttg
    28561 ttaggcacgc gcaaaccggt gcaactcatt tccggttatc gttccattga taccaacaat
    28621 gaactacgcg cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg
    28681 atggatttcc atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg
    28741 cgcgcaggtg gtgtaggata ttacccacgt agtaactttg tgcatattga taccgggcca
    28801 gcacggcact ggtagtaatt gcttaacgaa acaggggcag tatgaactat cgtattattc
    28861 cggtcaccgc attctcccag aactgttcat tgatctggtg tgaacaaacc cgcctggccg
    28921 cactggtcga tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc
    28981 tgacactgat gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg
    29041 aactggcgca acattacggc gtgccggttt ttggtccgga aaaagaagat gagttctggc
    29101 tgcaaggctt gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc
    29161 cagatcgttg gctgaacgaa ggcgatacca tcagcatagg gaatgtgact ttacaggtgt
    29221 tacattgccc tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc
    29281 tgatttctgg cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg
    29341 atcataatca actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga
    29401 catttattcc gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct
    29461 tcctgcaaga cgaaatgccc gtctggtaag gcacacataa aaaagcccgc ttttcagcgg
    29521 gcttcattgt ttttaacgct tacagcactg ccacaatcgc ttcgcacagc ggagccatgt
    29581 tatctggtgt catcccggcc acgtttacgc gaccagaagc aacagcatac acgccaaact
    29641 cttcgcgcag acgcagcact tgttcttttg tcaggccact gaaggagaac atgccgttct
    29701 gtttgatgat aaagctgaag tcgcggtttg cgcctttttc ctgcagcgta ttgacgaaca
    29761 actgacgcat acgctgaata cgctggcgca tatcagtcag ctcttgttcc caaatcgcac
    29821 gtaacgcatc gttgctcagg atggtggcaa caacagaagc gccgtgtgct ggtgggttag
    29881 agtagttagc gcgaatcgcc gctttcattt ggctgaatgc gcgatcaacg gtttcactgt
    29941 cggcagcaac cagagtacaa gcgccaacac gctcgttgta caggccaaag tttttagagt
    30001 aggaactggc aacaatcagc tctttatgca tagctgcgaa agcgcgcagt ccttcagcat
    30061 cttcttccag accacgggca aacccctggt aagcgaagtc aaacagcggt aaccagcctt
    30121 tctcaacgga gagttgtgcc agtgtctgcc attgttccag cgtagggtcg ataccggttg
    30181 ggttatggca gcagccatgg aacagcacta catcgccagc ctgagcttcg ttcagactgt
    30241 taatcagtgc atcgaagtca agggtgtgat tttccgcgtc ataataagcg tattcacgaa
    30301 cttccagacc tgcagagtta aagacgctct tatggttcgg ccagcttggg ttgctcaccc
    30361 acacacgctt aacgctggta ttttttgcca ggaaatcggc agctacgcgt agtgcgccag
    30421 tgccccccgg agtctgtgcc gtgcgagcac gtttgtcatt gatcagggcg ctacctttac
    30481 caaatagcag ttcctgagtg cagcgaccaa attcagggat gccgtcaatg ccgaggtaat
    30541 ttttggtggt ttcattttcg agcagatact gttcagcctt tttcacgctg gtcagtaccg
    30601 gggttttgcc cgtctcatct ttatagacac caatcccgag gttaattttg ccgggacgtt
    30661 catcggcacg aaacagatcg gccaggccca gaatcgggtc ggcaggagcg gcggtaatgt
    30721 tctcaaacat gacgaggttc cattatggtt acagaaggga agtccgctat cagggtaacg
    30781 ggagatttac aaaattccaa ctattactga tgaaaacgca ggctgttttt gcaagacgtg
    30841 agattgctct ggaaggcata aaaaaaacag gaccaacgtc ctgttttttc ggcatttaac
    30901 aaagaggtgt gctattagaa ctggtaaacg atacccacag caacggtgtc gtcagaacct
    30961 acgcccagtt tgttgtcaga atcgatctgg ttgatgatgt agtcaacata ggtggacatg
    31021 tttttgttga agtagtaggt tgcgcccact tcaaagtagt tcaccagatc aacatcaccg
    31081 ataccttcta cgtctttcgc tttagatttg gtgtaagcga tggacggacg cagaccgaaa
    31141 tcgaactgat attgcgcaac taacagaacg tcttgcgttt tgttggcgaa gccgctgatg
    31201 tttgtaaatt tattagtgat cggcgtagcg ttacgggttt caccgtagtt cgctgccagg
    31261 tagatgttgt tcgcgtcata cttcagacca gtagcccact gttcagcttt tttaccgttg
    31321 ccaagaagtt gagcttcttg caggttggta cggtcagctg caccataagc accaacgata
    31381 ccaaagcctt cgtattcata gctgatagaa ccgccaacac cgtcgccgtt agagcgacgt
    31441 gcagtgtcac gctcgttttt acccaggtac tgaacagcga agttcaggcc gtcaaccaga
    31501 ccgaagaagt tggagttacg ataggtagca acgccgccaa cacgaccaac gaagaagtca
    31561 tcgctgtatg cagtatcacc accaaattct ggcagcatat cggtgtaacc cagtgcatca
    31621 taaaccacac cgtagttacg gccgtaatcg aaagaaccaa cgtcagcgta tttaagaccc
    31681 gcgaatgcca gacgcgtttt gttaccagtt tgagcgtcag cgccttcaga gttgttaccc
    31741 tggaagttat attcccactg accataaccg gtcagatcgg aattgatttg agtttcccct
    31801 ttaaaaccaa gacgggcata ggtcatgtcg ccattgccac cgtaactgtt ttcaccgtta
    31861 cccttggaga aataatgcag accgacagct ttaccgtaca gatctacttt gttgccatct
    31921 ttgttataga tttctgcagc gtttgcagta cctgctacta acagagcagg gacgatcact
    31981 gccagaatat tgcgcttcat cattatttat taccctcatg gtttttttta tgacacctgc
    32041 cactgccgtc aataatttct gtcaataaaa atttacggaa ctattgatga gagtttggtg
    32101 tctttatgtg tctgcaggca tctttccatt caaactaacg tttcgcta
//
LOCUS       DAZFFG010000042        31810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFFG010000042 DAZFFG010000000
VERSION     DAZFFG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.042"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(70..741)
                     /gene="queE"
                     /locus_tag="ACHJ7J_002316"
     CDS             complement(70..741)
                     /gene="queE"
                     /locus_tag="ACHJ7J_002316"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO5000545.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            914..1516
                     /locus_tag="ACHJ7J_002317"
     CDS             914..1516
                     /locus_tag="ACHJ7J_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO5000546.1"
                     /translation="MLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRA
                     DLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQAPGDAQKLQAWQQAQAQV
                     TRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPEDVAAVSKAPTIDFSQNANAH"
     gene            1530..2201
                     /locus_tag="ACHJ7J_002318"
                     /pseudo
     CDS             1530..2201
                     /locus_tag="ACHJ7J_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            2438..3586
                     /locus_tag="ACHJ7J_002319"
     CDS             2438..3586
                     /locus_tag="ACHJ7J_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5000547.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSIVCFSLLLATTCKQRLAVVFNLCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            3583..4485
                     /locus_tag="ACHJ7J_002320"
     CDS             3583..4485
                     /locus_tag="ACHJ7J_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5000548.1"
                     /translation="MIVMRLIFILLALWSLPGLAQQIAVPELRQQVTDITGTLRTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKERASRKGSSSSSSG
                     GGSSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4545..5843)
                     /gene="eno"
                     /locus_tag="ACHJ7J_002321"
     CDS             complement(4545..5843)
                     /gene="eno"
                     /locus_tag="ACHJ7J_002321"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO5000549.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(5931..7568)
                     /gene="pyrG"
                     /locus_tag="ACHJ7J_002322"
     CDS             complement(5931..7568)
                     /gene="pyrG"
                     /locus_tag="ACHJ7J_002322"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO5000550.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(7796..8587)
                     /gene="mazG"
                     /locus_tag="ACHJ7J_002323"
     CDS             complement(7796..8587)
                     /gene="mazG"
                     /locus_tag="ACHJ7J_002323"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO5000551.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFSSIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(8659..8994)
                     /gene="mazF"
                     /locus_tag="ACHJ7J_002324"
     CDS             complement(8659..8994)
                     /gene="mazF"
                     /locus_tag="ACHJ7J_002324"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO5000552.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8994..9242)
                     /gene="mazE"
                     /locus_tag="ACHJ7J_002325"
     CDS             complement(8994..9242)
                     /gene="mazE"
                     /locus_tag="ACHJ7J_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO5000553.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9320..11554)
                     /gene="relA"
                     /locus_tag="ACHJ7J_002326"
     CDS             complement(9320..11554)
                     /gene="relA"
                     /locus_tag="ACHJ7J_002326"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO5000554.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(11602..12903)
                     /gene="rlmD"
                     /locus_tag="ACHJ7J_002327"
     CDS             complement(11602..12903)
                     /gene="rlmD"
                     /locus_tag="ACHJ7J_002327"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO5000555.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYSRAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            12960..15716
                     /gene="barA"
                     /locus_tag="ACHJ7J_002328"
     CDS             12960..15716
                     /gene="barA"
                     /locus_tag="ACHJ7J_002328"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO5000556.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(15949..17289)
                     /gene="gudD"
                     /locus_tag="ACHJ7J_002329"
     CDS             complement(15949..17289)
                     /gene="gudD"
                     /locus_tag="ACHJ7J_002329"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO5000557.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17310..18650)
                     /gene="gudX"
                     /locus_tag="ACHJ7J_002330"
     CDS             complement(17310..18650)
                     /gene="gudX"
                     /locus_tag="ACHJ7J_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO5000558.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPVVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(18652..20004)
                     /gene="gudP"
                     /locus_tag="ACHJ7J_002331"
     CDS             complement(18652..20004)
                     /gene="gudP"
                     /locus_tag="ACHJ7J_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO5000559.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(20439..20888)
                     /gene="yqcA"
                     /locus_tag="ACHJ7J_002332"
     CDS             complement(20439..20888)
                     /gene="yqcA"
                     /locus_tag="ACHJ7J_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO5000560.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKTKVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(20906..21688)
                     /gene="truC"
                     /locus_tag="ACHJ7J_002333"
     CDS             complement(20906..21688)
                     /gene="truC"
                     /locus_tag="ACHJ7J_002333"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO5000561.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(21688..22017)
                     /gene="yqcC"
                     /locus_tag="ACHJ7J_002334"
     CDS             complement(21688..22017)
                     /gene="yqcC"
                     /locus_tag="ACHJ7J_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO5000562.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(22639..23184)
                     /gene="syd"
                     /locus_tag="ACHJ7J_002335"
     CDS             complement(22639..23184)
                     /gene="syd"
                     /locus_tag="ACHJ7J_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO5000563.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23252..24100
                     /gene="queF"
                     /locus_tag="ACHJ7J_002336"
     CDS             23252..24100
                     /gene="queF"
                     /locus_tag="ACHJ7J_002336"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO5000564.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            24212..25576
                     /gene="ppnN"
                     /locus_tag="ACHJ7J_002337"
     CDS             24212..25576
                     /gene="ppnN"
                     /locus_tag="ACHJ7J_002337"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO5000565.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            26133..27422
                     /gene="sdaC"
                     /locus_tag="ACHJ7J_002338"
     CDS             26133..27422
                     /gene="sdaC"
                     /locus_tag="ACHJ7J_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO5000566.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            27480..28847
                     /gene="sdaB"
                     /locus_tag="ACHJ7J_002339"
     CDS             27480..28847
                     /gene="sdaB"
                     /locus_tag="ACHJ7J_002339"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO5000567.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            28959..29714
                     /gene="xni"
                     /locus_tag="ACHJ7J_002340"
     CDS             28959..29714
                     /gene="xni"
                     /locus_tag="ACHJ7J_002340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO5000568.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(29769..30917)
                     /gene="fucO"
                     /locus_tag="ACHJ7J_002341"
     CDS             complement(29769..30917)
                     /gene="fucO"
                     /locus_tag="ACHJ7J_002341"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5000569.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGEKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(30945..31592)
                     /gene="fucA"
                     /locus_tag="ACHJ7J_002342"
     CDS             complement(30945..31592)
                     /gene="fucA"
                     /locus_tag="ACHJ7J_002342"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO5000570.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         7806 a         8351 c         7634 g         8019 t
ORIGIN      
        1 cgacaaagaa tataaatgag tagaagtctt tacaggttcc acccatttat aacgcttata
       61 aaagtttaat caggcaatat ttaaatattt atgggtttgc atcgacaaac gccaattacg
      121 cgcaatgcag gtttcaatgc acaaacgtgt ggcatcatcc ttttggctaa tcggctgcag
      181 tgcaatgact cgcggtttat catcggtcag cgttgccagt agttcatcca gtgcttcaat
      241 atcgcgtacg cgccccactg gatgcttgat ttcgttggct cgctccagtg cctgtgacaa
      301 cacttcatag ccgccgcgca tgttcagctt tggcgatacg gtaacccagg tattcggtgt
      361 gcagcgtact tcatgagtac cactggtttc gatctggcag ctaaaaccgt tcttttcgag
      421 cagatcagtc agtggtagca aatcatgaat gcaaggctca ccacccgtaa tcaccacatg
      481 ccgcgcggtg tatccctggc gaccaatgac agccagcaaa tcttcactgc tcgccgcccc
      541 ccacttatca ctctctttgg tcttcgccag aatgctgaaa agggagactt cccgatcctc
      601 aagcttttcc caggtgtgtt tggtgtcgca ccaggcacag ccaaccgggc atccctgtaa
      661 acgaataaaa atggcgggga cgccggtaaa gtaaccctca ccttgcaggg tctggaacat
      721 ctcgttaatc gggtactgca tagcattctc tgtgaagtgg ataattgtta attattgcag
      781 atcctgccgc aacaatcatg tcttattaac attctgttac aggcaggttt aaggcaaaaa
      841 gtgcattaga tcataaagaa taaaatgaag cggcgtatta tgttttaagg aattaatgaa
      901 aaaggattta aacatgctca ggatattcat cgtaattata tttattttca atttatcagg
      961 ctgtggatat aacgatattc aaacctatga tgagcaggtt aacgcctcct ggtctgaggt
     1021 attgaatcaa tatcaacgtc gcgccgatct tattcctaat cttgtcgcca gcattaaagg
     1081 ttattccagc catgaacgag atgtactgga agccgtaaca ttggctcgta gccaggctaa
     1141 tcgcgccagc agcgatcttc agcaagctcc cggagatgca caaaaattac aagcctggca
     1201 acaggctcaa gcgcaggtga ctcgcactct cggtcagtta acgataatta gtgagcgtta
     1261 ccctgagtta aaatctcagg aactctatca aaatttaatg gtacagcttg aaggaagtga
     1321 aaatcggatt gccgttgcgc gaggaagata tattaaagcc attgaacaat ataatgtcac
     1381 tatccgcaaa ttccccgctg tgttaaccgc aaaggtcatg gattacacac caaagaaaaa
     1441 ttatttgccg gaagatgtcg ccgcagtaag taaagcccca acaatagatt ttagccagaa
     1501 tgctaatgct cattaagcgg ggaagttcta tgcgaaattt tatcttcctt atgtctttct
     1561 tctgttcatc tgtgtttgcc acacaaattc cagtagctga atcccccaag tatgtgaatg
     1621 acttaaccgg cacattaaca aacagcgaag taaacaccct gaccaaccag attaaagccc
     1681 tgacgcaaaa aaaccacgca cagttggtcg tgttagtggt tgatacaaca ggcgatgaaa
     1741 ccatcgagca gtacgcaacg cgggtattcg atagctggaa accgggtgat aaagaccgtg
     1801 atgatggcgt tctactgtta gtcgcctggc aggatcatac cgtgcgtatt gaaatcggtt
     1861 acgggctgga aggtattatt acggatgcac agtccgggaa aattattcgc aacagtatta
     1921 ttcctgcatt taaaaaaggc gacctggctg gcggtttaca aaaggggatc agcgatattg
     1981 aaagtcgtct tacggggaat aatttagcaa cgataacacc gaccgatcac cctttgccct
     2041 ttagcggttg gtgggcattg ttagtctggg cgatagtact gacgtttatt tctgccagag
     2101 gatatatcaa aacgctggga gtaatttgct tcgcagctat tgtactggcc tttgttttac
     2161 ctatagcagg cttcagcgga agctggggag tactcgcaac attactctgc ttcgccacac
     2221 cgttcctggc tgtcgccatc gcttttaccc ccttcggcaa aaaagttcgg gatttgatgc
     2281 gtgatgtcaa ccagccatca cgccatacaa gaagcaattc ttcctcccac tattcatcat
     2341 cgtcatcctc ttcattcaac aatgacaatt tcagcggtgg tggcggttca tctggtggcg
     2401 gtggcgcttc cgggcgctgg taacaaaagg aattgatatg cgtatttttc tcttacttct
     2461 gatgctatgc ggttttcagg tatttgctga acgattgcct tcagtgactg acttaaccgg
     2521 gacattaacg acagaagaac aaactgcttt aactcaacaa ttacagactc tcgagcagca
     2581 aaaccacttc caggtggccg tgttggttac gccgaccaca gggggcaaaa acattaaact
     2641 tgcagcctcg cagagatacg gggtatatca ctggaaaccc gttggagaga agcgatatgg
     2701 cgaaggtatc ttaattctcg tcgtatggcc ggaagggctg gcgtcaatga agattggaca
     2761 tggactggaa gagatgttgc cgccggaaca agctgcgcaa attgttcgtt accatatgca
     2821 gccagaattc gaaaaaaaca atctctttgc tggattaacg ggcggaattg aaagcatcgc
     2881 acagtttacc catatcaaag cgaaccttag ccctttggat gctttagcca atcatctgtt
     2941 tgctaatccg caacgttcgc tgccatgtct tgcctggaca ctgctgatga tcgttgccat
     3001 cgttgtcctc tggcgatttg ccagccgccc acggcgtggc atatggatga tatcgatgat
     3061 tacccctatg gtctggtttt tctcctttca ggacgatgtc atcattcggc gggtgtcgat
     3121 cgtttgtttt agtttattgt tagcaacaac ctgcaagcag cgtctcgcgg tagtttttaa
     3181 tttgtgccgc gtatttccta tgatgctggc cccaaaaaat aaaaacgcta aagtgaaaaa
     3241 acaaaagaag actccgcagg ttcgtgaatc aggacgaaat tcattgtttg tcgtgatgct
     3301 gataattatt gtgggatggt gcctggtgtt cgccagtaat aaagatattg cattcatctc
     3361 aacaattatt gggttaattg atcattttat cggcccaata accattgctg gcatcgttct
     3421 gctgatcatt gtggccaaat ttactggcaa tcttaagctg gggtcaggag aaaaaagcaa
     3481 caggaaaacg gggaatcgta atgctcattc tcgctcttcg tcgagaaatt ctttccgcgg
     3541 tggtggcggt tcttcaggag gcggcggttc atccggccgt tggtgatcgt aatgcgctta
     3601 atctttattc tacttgcttt gtggagcctt cccgggcttg cccagcaaat tgctgtgccg
     3661 gagctgcgcc aacaagtgac tgatattact ggtacgttaa gaacgtctga acaacaatca
     3721 ctgacacagc agctgcagga tattacacat aaaacacggg cgcaggtcgc tgtattagta
     3781 gtgccgtcca ccggagacga ttccattgaa caatatgcta caagggtttt tgatagctgg
     3841 aaactgggag ataagcagag aaacgatggt attttacttc tggttgcctg ggaggatcat
     3901 gctgttcgta ttgaggttgg ctacgggctg gaaggggtgg ttaccgacct gcaagcggcg
     3961 aagattatca gggatatatt gatccctgcc ttcaaaagtg atgacctgat gggtggatta
     4021 acactggcaa gcgaaaatat cggcgcgctt ctgttgaatg gtgaattacc ggaagacagg
     4081 ggggattatt acagtatcaa tccccctatt ccattatcgc ttgctgtaat tatattgctg
     4141 gcggtacttt catattttat tgtttttacc gatccgtcga atttaccctg gatcactctc
     4201 actggcgcca tttacgggat ggtattcctc tatgttgccg agccaggacc atggaccaat
     4261 ttaatcgttg cctgcggtat gttaacgcct tttgcgatcg taccactggt tattttttgg
     4321 cttatcgtca ataaaaagct acgcgccaaa tacaaaaaac tcagtaaaga aagagcttca
     4381 agaaaaggtt cttcttcatc ctcttccggt ggaggctcaa gtggcggcgg atttagcggc
     4441 gggggcggtt cttctggagg tggcggcgca tccggccgct ggtaactcaa ccataaaaaa
     4501 tgccagcctc cgggctggca tttttaaatc agataaagtc agacttatgc ctggcctttg
     4561 atctctttac gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc
     4621 tggttgtatt tagcaacgcg gtcagaacgg ctcatagaac cagttttgat ctggcctgca
     4681 gcagtaccaa cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag
     4741 ataactgcag tgtagccagc gtctttcgcc atcttgattg cagccagagt ttcggtcaga
     4801 gaaccgatct ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct
     4861 ttcaggatct tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc
     4921 agaactttgg tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata
     4981 gaaacgatcg ggtactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg
     5041 aaagctttgt tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagcc
     5101 gcgcagtcca tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct
     5161 tcagcgataa cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg
     5221 tcaccaacag cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact
     5281 tcagaaccca tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat
     5341 tcctggatat caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga
     5401 accggcatgg agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc
     5461 atacctttag cagctgcagc agctttggcg ttagccagag atacagccag gattgcgttc
     5521 gcgccgaatt tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca
     5581 gcctgatctt tagcatcttt gccaatcagc gcctgagcga tcgggccgtt taccgcagca
     5641 acagctttgg ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga
     5701 gcttcacggg aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc
     5761 tccagatgta cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg
     5821 atgattttta cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc
     5881 agacaaacaa cgcgtaccca gggtacgcgt tgccgttcta acttttttac ttacttcgcc
     5941 tgacgtttct ggaactcgct ggcggctttc acaaagcctg caaacagcgg gtgaccatca
     6001 cgtggagtag aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga
     6061 acttcgatga tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca
     6121 tcttcaatct gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca
     6181 attgtcggcg cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt
     6241 gcgccgagac gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg
     6301 ttttcatcgc gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat
     6361 tccgtagagt tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc
     6421 tgcataccca ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc
     6481 gtaatcatgc cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga
     6541 cctttaagga tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg
     6601 acgctgacac gattcttcag cccaccgtgt ttcagtgctt cgatcacgga tttataagca
     6661 tccggcagtt caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct
     6721 tcttcgaaga taacctgttc ccattcggac agattcgctt ccgggcagtt taagctgaat
     6781 cgtttacaaa tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg
     6841 gaatcgacgt ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc
     6901 ttcgcacgtt cgttcgccgg aacggcgcga tctgaacgac aaatcaggat gtcaggctgg
     6961 ataccgatgg agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca
     7021 gacgctgcca tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt
     7081 tcaacagcca tctggcgaat cgcttcgagg aacggcaggg attcgatatc acctactgta
     7141 ccgccgattt cgaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg
     7201 attgcgttag tgatgtgcgg aataacctgt acggttgcgc cgaggtagtc accgcggcgt
     7261 tctttacgca gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt
     7321 ttggtacgga tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca
     7381 gtaacgaaca cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac
     7441 ggatccagtt tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag
     7501 gctgcggcaa tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc
     7561 gttgtcatgc tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa
     7621 agcagtatac ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc
     7681 gcactattga aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa
     7741 tcaagcgctt gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga
     7801 gatcaatttc ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac
     7861 ctgtcatttc cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga
     7921 atttttcgtt cgctttttgc aatgcgattt ccgcttttgt ccctaaatga cgagccagat
     7981 tgaccgtggc aaacagcagg tcacccattt cctcctccag tttagcctgg tcgataacag
     8041 cctgccgtgc ctcgtacatc acctcgtcga tctcttcgta gactttatca accactgggc
     8101 caagcgtcgt ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca
     8161 ttaaagccgg taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc
     8221 gctcttcggt tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac
     8281 tatcagcaaa aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat
     8341 cattaaagtc aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata
     8401 gcagatcgcc cagttcaccg cgcagatcgt caaagtcttc gcgggcaata gcgtccagca
     8461 cctcgtaggt ttcttcaagg gtgtaaggcg caatagaaga aaatgtctgc tctttatccc
     8521 acgggcagcc gttttccggg tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt
     8581 gattcattag aatgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata
     8641 gaagtgagtt agtaacacct acccaatcag tacgttaatt ttggctttaa tgagttgtaa
     8701 ttcctctggg gcaaccgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt
     8761 tacctgatca gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg
     8821 atatcctttt gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta
     8881 catgaacgga ctcaggacaa cagccggacg atgtccggct tgctcgctac cttttgtcgg
     8941 gtcaaaatca acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag
     9001 acttccttat ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg
     9061 tcgttgacca gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat
     9121 ttgccatcca ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt
     9181 aacgtagccg ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc
     9241 ataacccttt cctcaaacca ctatcatatg tagatacagt atatatcaat ctacattgta
     9301 gatacgagca aatttcggcc taactcccgt gcaaccgacg cgcgtcgata acatccggca
     9361 cctggttgag tttacccagc acgcgcccca gcacttgcag gttgtaaatc tcaatggtca
     9421 tgtcgatggt cgccagttgc tgtttggtgt cgctacggct ggcaacgcca agcacgttca
     9481 ccttctcgtt ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca
     9541 ccacgcggac caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa
     9601 tgcgttctgg cgcatgggag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg
     9661 aaataccgcg tccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc
     9721 agcgcgcgat gtggtgcatc aggttaccga caccttcaac caccacacga ccgttatctt
     9781 tactgcggtt ttgcggtgtg tagctttttt gctgaagctg tttcagcgcg gcggcgtcct
     9841 gctcttcggc actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac
     9901 ggatatcccc gccaccaatc gccgccagca actcgtcgac atcattgaag ttgtaacgcg
     9961 gcagcagatg tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa
    10021 ggatttgccg cccagccaga atgtttttgt cacggtcctg tttacggaac caggcgtgaa
    10081 ttttcgaacg cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc
    10141 tggggttcgg ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg
    10201 tgaacggcac aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt
    10261 ggatgtggta agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt
    10321 tcggcgtaaa gacatacacc cggtcgtcaa acacctgact acgtacttcg tcgagcattt
    10381 cgccggaatc agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt
    10441 cttcatgtcc cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag
    10501 caacacccaa ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt
    10561 ttccacccgg ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag
    10621 cgacgtaatc atcaaactca tccggcaggt ggcgatagtg tgtgtgcact atccccagtg
    10681 cggcataaca atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct
    10741 catcgaaggc gaggttcttt ttctgcattt tgcgccagat gctgtagatg tgtttcggac
    10801 gaccatacac ttcagcttta acgccttcag ctttcatctc agcgcgcaga tgaccgacaa
    10861 actcttcgat atagtgttcg cggtcgagac ggcgttcatg caacagtttt gcgatgcgtt
    10921 tgtattcggt cgggtggaga taacggaagc agtaatcttc cagctcccat ttcagttgcc
    10981 caatcccaag acggttcgcc aacggcgcgt agatattggt gcactctttt gccgccagta
    11041 cgcgttcatc ttccggcgca tcttttactt cacgcagatg agcaatacgc tccgccagtt
    11101 tgatgaccac gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga
    11161 cctgttcgga ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat
    11221 cacgcacgcc gtgaataagg ttaacgaccg acttaccgac gctctcacgc agcacatctt
    11281 cgctgactac gttggcatcc gccagaggga aaagcagcgc cgcccgcagc gtgtcaatgt
    11341 ccatacttaa tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg
    11401 catccggatg cccctgcgtc tgttgcagac aatacgccca ggtttcggct aagcactcac
    11461 acgacttctg gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag
    11521 ccttattgat atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct
    11581 gccgaaatcg gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg
    11641 tcccgtgtgt gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa
    11701 taacgcttcg ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat
    11761 aggttccagt tttataattt gctgcataac acctgcggca cctgctcgcg cggggtccag
    11821 caacacttta tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt
    11881 ttcatgataa aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc
    11941 caccagcgca ggaacacctt ctacacccac cacactggct gcttgtgtcg ccaatggcag
    12001 tgtaaagttg cccataccgc agaacagatc cagtacgcga tcttcaggtt gcacatccag
    12061 ccattccaac gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc
    12121 gcgcgggcta aaagttaagc gcaacccatt tgagtcatac cagggcatct caccagagac
    12181 ggtttcgagt atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa
    12241 gcgttccagt ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag
    12301 cgtgccgctg gttgcctgta ccagttcaac atgaccaaga tggcgcatag cttgtaagct
    12361 gcccaggcat gccctgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca
    12421 ttgtttaacg tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg
    12481 tgttttcggt aagtaattca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc
    12541 ggcgatcact tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg
    12601 ctgctgtaaa tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg
    12661 acagcgtggc gtttcgcgtt ccgggctatc gcttaaccgg cgtacgactt tagcgcggga
    12721 atactgtttt ttatcttcag taacagtaac ttccgcgttt tcctgcggta ataatccagg
    12781 gataaatagc gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt
    12841 gactgaaacg gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc
    12901 cattggcgag actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca
    12961 tgaccaacta cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta
    13021 ttggtttatt gctgagtatc tttttcgtcg tgcatcgcta taacgacttg cagcgtcaac
    13081 tggaagatgc cggtgccagc attattgagc cgcttgcagt ttctactgaa tatggcatga
    13141 gcctgcaaaa tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg
    13201 atattgttcg cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt
    13261 ttcatcttga tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca
    13321 ctgtcactcg tgacggcgat attatgatcc tgcgcacgcc gattatttct gagagttact
    13381 cccccgacga atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg
    13441 cgcttgagct ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca
    13501 gcgtgatgat gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg
    13561 atgtaaccgg tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac
    13621 tcgacagccg ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca
    13681 actcgatggc aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg
    13741 cgacgtccga tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttggatc
    13801 tggcgaaaaa gcgcgcccag gaagcggcac gtattaaatc cgagtttctg gcaaatatgt
    13861 cacacgagct gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa
    13921 cagaattaac accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt
    13981 tgctggcaat tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc
    14041 tggaaagtat tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac
    14101 attcttctca cgataaaggg ctggaactga cgctcaatat taaaagcgac gtgcctgata
    14161 acgtgatcgg cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa
    14221 ttaaattcac cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata
    14281 ccaaagtgca gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc
    14341 aatcgcgctt attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg
    14401 gcaccgggct ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt
    14461 cgttccatag ccagccgaat cgcggatcaa ctttctggtt ccacattaat ctcgatctga
    14521 acccgaacat tattattgaa gggccatcca cccagtgcct cgccggtaaa cgcctggcct
    14581 atgtcgaacc aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc
    14641 tggaagtggt ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt
    14701 tgttaggcat cgcggtgacc ttccgcgagc cgttaacaat gcaacatgag cgattagcga
    14761 aagcggtatc gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg
    14821 ctgaaaaact caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc
    14881 gcctgctgcc tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa
    14941 ccgatgaaag taagctggca atgacagtca tggcggttga tgacaacccc gctaacctga
    15001 aacttatcgg cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc
    15061 atcaggcggt tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa
    15121 tgccggacat ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac
    15181 aaacgccggt tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg
    15241 gcgcaggaat gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt
    15301 tgttgcgcta caagcctggc agcggtattt cgtctcgcgt cgtgacgccc gaagtcaacg
    15361 aaattgtggt gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag
    15421 gaaaaaccga tttagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc
    15481 gcaacaaagt tgaggaacag ctggttggag aaaacccgga aggcctggtt gatttgattc
    15541 ataaactgca tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac
    15601 tgatcgaaca acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg
    15661 aactgttgga cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga
    15721 cgtcttgtcg agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc
    15781 ctgtgtttat gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc
    15841 aataaattaa aaagcatgta ggcctgataa gcgtagcgca tcaggcaatt ttgtgtttgg
    15901 catcagtctc aaaccggctc ctgacagagc cggttttggt tttctgtctt aacgcaccat
    15961 gcacgggcgc ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc
    16021 gtcacgcgcg ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat
    16081 atcgatttct acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc
    16141 aaacggttct ttggtcaggc gctgattgcc ttcctgccaa atccagtgcg tatcaatagc
    16201 agtaatttta cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa
    16261 gtggttgtta gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg
    16321 taccgaacct tgcatcgtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa
    16381 cgagagcgta tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag
    16441 gccagtcgcg cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc
    16501 acacggatct tctgcataag ccagcgaacc tttcaggtat ttaccaattt taatcgcttc
    16561 gttcagcgac caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc
    16621 cagtgccaca atagactcgg cctcttcttc cccggccagt acaccgcctt tcagtttgaa
    16681 atcgttgaaa ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt
    16741 catcgcttct tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata
    16801 cggcagcggt gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc
    16861 gctacgctgt tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag
    16921 cagatccagc attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa
    16981 tgtctgcaaa ccgcgcccgc cggcatcacg atcggcaaaa gtattacgca ccagcgtcag
    17041 aacgtttttg tattcaccca gcgttttacc cactaccagc ggaatcgcat cttccagcgt
    17101 tttacggatt ttctcgccgc cgggaatttc ccctacgcca gtgtgaccag aattatcttt
    17161 gataatcaca atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat
    17221 actgtcatgg cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg
    17281 agaactcata tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg
    17341 tcaaaggtcc agccggggat caggtactgc atcgggcctg cgtcgttacg cgcaccgcca
    17401 ggcagacgtt tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc
    17461 agaccgggcg catcaggaac ggcaattttt ccgtttttaa tctccagcgg attttgggtc
    17521 aggcgacaat cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggc
    17581 gccgccgcgc ccacatgggt aaacatcgcc agagagatat cgaaatggtt attagaatgg
    17641 cagccccagg tcagccccca gtcgtcgcaa agctgcgcca cacggactgc accggaaagc
    17701 gtccagaagt gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc
    17761 atttcgcgcc agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgccga
    17821 aactccgcca tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca
    17881 taggtaagaa catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca
    17941 ccgttgggat caacggtaat ccgcgcatcc ggaaagcgtt tcttcaatgc acgaacagtg
    18001 tcgatttctt gctcgccagg taacacgccg cccttaagtt tgaaatcttt aaagccatag
    18061 cgatcctgtg aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg
    18121 cgcaactgat accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg
    18181 gtccgatcac cgatataaaa cagataaccg aggacggtaa tagcctcgcg ttgcttgcct
    18241 ggccctaaca gttcgcagac cggaacattc agcgccttac ctaacaggtc aagcaaggcg
    18301 gcttccagcg ccgctacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag
    18361 gtatcaaaat cggctgcctg attaccttta tgcacctgct gaaccacttt attcaggcgc
    18421 gcgacttcct ggcccagaac caccggaata gcatcgacaa gcgtctgata aatcacctct
    18481 ccgcccggcg cttcaccaat gccggtatgc ccggcattat cggtgagtac cacgatattg
    18541 cgggtgaagt atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg
    18601 accggaatga ctttcatatc agtaataaca ggactggatt gtgtcgtcat cttattgccc
    18661 cgcgacaggt ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac
    18721 cgcgattaag gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc
    18781 aacgatataa ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa
    18841 caggccaccg gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc
    18901 cagcgccccg atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc
    18961 aacgttgacg tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg
    19021 tgttttacgc gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataatccc
    19081 acccagcaca ccaccgataa aaccacaaac cgccggaacg gaagccacaa agcccgcttt
    19141 cagaatcgac atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta
    19201 agtcagggcg ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag
    19261 cagttgtttg atctgccccc acttcacgct gaacggcact ttaactttgg tgttttgctg
    19321 atccatattg atcagcgcgc cacccgcggc gatgtactcc agctctttct gatttacccc
    19381 cggatgttga tttggctcgt ggatgacttt caaccagata aagctgatga caatccccag
    19441 accgcccata aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat
    19501 cggcgcgaag atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc
    19561 cctttcctgc gccggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc
    19621 ttcagcaagc ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact
    19681 aaagatatcg acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa
    19741 gtagacgcgt tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac
    19801 ataagcccat gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc
    19861 tttggccatt tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt
    19921 gacgataaac aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct
    19981 cgcagcctga cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca
    20041 ctctggactg cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt
    20101 ataggtcgtt cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc
    20161 gccatagcgg ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg
    20221 gcattagccc tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa
    20281 gggttaaaac gaagaaataa agaagagtat gaaatggatc gcttgactcc aggcaaacgc
    20341 cagtaaaaat ccgcgctatg aagcagtttt tactggcatt tgcctgaaga gattcgattc
    20401 agcaccgcta aaacgacatt taccgctcgc tgaacatatc aggacaacag cgtgccccac
    20461 tgttcgaccc acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac
    20521 agcatttcgc caacccgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg
    20581 ccattgcaga aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc
    20641 ggctggaagc ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg
    20701 tcgccctgcc cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag
    20761 tcgcttaatt caggatcttc aaataccttt gttttgtggc cctgtgcggt cagaattgct
    20821 tctgcttctt ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt
    20881 tccgccatac cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg
    20941 cactgaactc aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg
    21001 cctgcatcca ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat
    21061 gcgtcaatga caactgactg gcatgcagca ttaagcgctg aaggccaaaa tgctcagcac
    21121 cgctgcgatt ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat
    21181 gggcaagatg gcgacggagc tggtgcttgc gtccggtttt cggctccagt tccaccaggc
    21241 cgtagcgcgt ggtcgggtaa cgtccggtcg ctacaggcat ttctacggtc gccagaccgc
    21301 gataatgcgt cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt
    21361 tgtccagttc ttccaccagt ggataatcca gcaccgcttc ttccatcaac cagccgcgca
    21421 caatcgcatg gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc
    21481 cggcctcgct ggataatccc atcaacaaca caccagaagt tggtcggtcc agacgatgag
    21541 cagtaaaaac atgctggcct atctggtcac gcacggtttg catgaccact actttctcgt
    21601 cgcgatccag ccagctgcgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc
    21661 attcatcctg atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag
    21721 tttttccaac tcagccagaa taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc
    21781 ataataggga gcaacggcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg
    21841 cattcgcggg atcagcaccc actgcaacca ctccagtggt tccatggtgt ccataaagaa
    21901 cggttgggta ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc
    21961 acgcagtaac gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa
    22021 gcaacctcaa taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt
    22081 attcacagtg ctcccggttc gtttcgcagc attccagcta catttgttgc tccctgctca
    22141 tccttgacaa cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct
    22201 gctcacacca ccccgatgtg atacttcatc ctgaagtgtc cctggccttc ctgacccacc
    22261 gaatcatcct gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt
    22321 catcctcttc gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtccttcag
    22381 aagtgtcatc atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc
    22441 gtctttttcg cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat
    22501 gaaagttagg aattaacaag caacatgttg tttttaaatg aaaataacga ttcttatctt
    22561 tttaagtatt cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa
    22621 ccagatgggg tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt
    22681 tgaggctaaa tgcgtccgtt tacgtgttcc cagcgtctct ttacacactt caccggagag
    22741 attacacacc gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg
    22801 cagttttagc cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa
    22861 gtcgtcttca ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat
    22921 atccccggca aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa
    22981 agcgcgttca accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac
    23041 ggcatcttca gtagttgaaa taatgcacgg tgaaggaacg ccgtacagtt cctcacttaa
    23101 cggccagctt ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag
    23161 ggcttgtgcg gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc
    23221 gtttagttta tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact
    23281 tgcgggcctg actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact
    23341 gcaaggcgtt ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc
    23401 ttttcaaggt acggacatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt
    23461 gccgcaggtc gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc
    23521 gaagagtttt aagctttatc tcaacagttt taaccagacg cgttttaata actgggatga
    23581 ggtgcgccag acgctggagc gcgacttaag cacttgcgct cagggtaagg ttagcgtggc
    23641 gttatatcgt cttgatgaac tggaaggcca gccgataggt catttcaatg gcacttgcat
    23701 tgatgaccag gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc
    23761 caccagtggt gaaaaagtag tggaagagac gcttgtcagc cacctgctga aatcaaactg
    23821 cctgatcacc catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat
    23881 tgacagagaa aaactgctgc gttacctggt ctctttccgc catcacaacg agttccacga
    23941 acagtgcgtg gaacgcatct ttaatgacct gttacgcttc tgccagccag agaaattgag
    24001 cgtttacgca cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag
    24061 cgattttgtc ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt
    24121 gctggattca cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga
    24181 atgacgatat tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc
    24241 catggatatg ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct
    24301 ctatcaattg tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa
    24361 cagcaaagaa ttgctgtctc gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg
    24421 cggcgtaaag ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat
    24481 tcgcgctttg caggccaact tgttcgcggt tctgcgagac attctcttcg tttacgggca
    24541 aatccataac accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa
    24601 cctggtcttt tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat
    24661 ggtggtctgc tggggcggtc actcaattaa cgaaaatgag tatttgtatg cccgtcgcgt
    24721 cggaaaccag ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc cgggagcgat
    24781 ggaagcgccg atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg
    24841 ttttattggt atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cgctggtcaa
    24901 cgaattgatc atcatgccag atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca
    24961 cggtatcatt atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg
    25021 aattttaatg aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa
    25081 agagagcgcc gactacttcc gcgtactgga cgagtttgtc gtgcatacgc tgggtgaaaa
    25141 cgcgcgccgc cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa
    25201 aaaatcgatg ccgctggtga aagaaaatcg ccgcgacact ggtgacgctt acagctttaa
    25261 ctggtcaatg cgcattgcac cagatttgca aatgccgttt gagccgtctc acgagaatat
    25321 ggctaatctg aagctttacc cggatcaacc tgttgaagtg ctggctgccg acctgcgccg
    25381 tgcgttctcc ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga
    25441 gtttggtcct tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct
    25501 acagggtttt gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta
    25561 cgaaatctgc acgtaatctc ccctcccccg gtgacgtttg ttgccgggtt ttctcgtttt
    25621 tggtcactta ctcatcaact catttcattt gttatatgaa tgtttcttac caccctcacg
    25681 cgacaaatat catcacagtt aatatgtcat acaatttata ttgcaacgca aacgtttccc
    25741 tattttcata aaccgtattt tatcgctatg aaaaagaaat tatcgccatg attaactaaa
    25801 agtattgatt ttttcagttc aacctacata tattgcgcgc cccggaagaa gtcagatgtc
    25861 gtttaatggg caaatattgc ccttaaattc tcttttactt ttgatttaca gagtaaagcg
    25921 ttgggataat ctatcttcca agtagattat tgtatttggg atcaagatca ctgatagata
    25981 cataacttgt gtgtatcttt ccgccctcaa attattacgg cggtaaatga ttaagccatc
    26041 gccgatagac agatttcatt tttacggtca ggcaccttcc cgggctgaac tggctaaaag
    26101 ctgaattatt tgcattcctc caggagaaat agatggaaac gactcaaacc agcacgattg
    26161 cgtcgaaaga ctctcgtagt gcctggcgca agacagacac catgtggatg ctgggccttt
    26221 acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt gttggcggta
    26281 tgatcccgct gatcatcatg gctatccttg cgttcccgat gacgtttttt gctcaccgcg
    26341 gcctgactcg cttcgtactg tctggtaaaa acccgggcga agacatcacc gaggttgtag
    26401 aagaacactt tggtattggc gcaggtaaac tgattaccct gctctacttc ttcgctatct
    26461 acccgatcct gctggtttat agcgtggcaa tcaccaatac agttgaaagc ttcatgtctc
    26521 accagctggg tatgacacca ccgccgcgtg cgattctgtc gctgatcctg atcgtgggta
    26581 tgatgaccat cgttcgcttt ggtgaacaga tgatcgttaa agcgatgagt attctggtat
    26641 tcccgtttgt tggcgtactg atgctgctgg ctctgtacct gatcccgcag tggaacggcg
    26701 cagcactgga aacgctgtct ctggacactg catctgcaac cggaaacggt ctgtggatga
    26761 ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa ccactctccg attatctctt
    26821 ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa tgctcgaaga
    26881 tcctggcatt cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc ttcagctgtg
    26941 tactgagcct gactccggca gacctggctg cggctaaaga gcagaacatc tcgattctgt
    27001 cttacctggc taaccacttt aacgcaccgg ttatcgcgtg gatggctccg attatcgcga
    27061 ttatcgctat caccaaatcc ttcctcggcc actacctggg cgcacgtgaa ggcttcaacg
    27121 gtatggtgat taaatctctg cgtggtaaag gtaagtctat cgaaatcaac aagctgaacc
    27181 gtatcactgc gctgttcatg ctggtaacaa cctggattgt tgccaccctg aacccgagca
    27241 tcctgggtat gattgaaacc ctgggcggcc caatcatcgc gatgatcctg ttcctgatgc
    27301 cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac atcagcaacg
    27361 tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat cttctactct ctgttcagct
    27421 aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt ttaacgcgat gtatttccta
    27481 tgattagcgt attcgatatt ttcaaaatcg gcattggccc ttccagttct cataccgttg
    27541 gaccaatgaa agcgggtaaa caatttaccg acgatctgat tgcccgtaac ctgcttaaag
    27601 acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc tctaactggt aaaggccacc
    27661 acactgatat cgccattatt atgggcctgg cgggtaacct gccggatacc gtggatatcg
    27721 attccatccc cggttttatt caggatgtga atactcatgg tcgcctgatg ctggcaaacg
    27781 gtcagcatga agtggagttc ccggttgatc agtgcatgaa cttccatgcc gacaaccttt
    27841 ctctgcatga aaacggtatg cgcattaccg cgctggcggg tgataaagtc gtttacagtc
    27901 agacttacta ctctattggc ggtggcttta tcgttgatga agagcatttt ggccagcagg
    27961 atagcgcacc ggttgaagtt ccttatccgt acagttcagc agccgatctg caaaaacatt
    28021 gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa aaacgagctg gcactgcaca
    28081 gcaaagaaga gctggaacag cacctggcga acgtctggga agtcatgcgc ggcggtattg
    28141 agcgcggtat ttccaccgaa ggcgtgttgc caggcaaact gcgcgtacca cgccgtgctg
    28201 cggcactacg ccggatgctg gtcagccagg ataaaaccac cactgacccg atggcggttg
    28261 ttgactggat caacatgttt gcactggcag tgaacgaaga gaacgccgct ggcggtcgcg
    28321 tggtgactgc gccgactaac ggtgcgtgcg ggattatccc ggctgtgctg gcgtactacg
    28381 ataagtttat ccgcgaagtg aacgctaact cactggctcg ttacctgctg gtagccagcg
    28441 ccattggttc tctttataag atgaacgcgt cgatttctgg tgctgaagtg ggttgccagg
    28501 gtgaagttgg cgtggcgtgc tcaatggcgg cggctggtct ggcagaacta ttaggcgcaa
    28561 gcccggcgca ggtgtgcatc gcggcagaaa tcgccatgga gcacaacctc ggtctgacgt
    28621 gtgacccggt cgccggacag gtacaggtgc catgcatcga gcgtaacgcc attgcggcag
    28681 taaaagcggt gaacgccgca cgtatggcgc tgcgccgtac cagcgagccg cgcgtctgcc
    28741 tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga tatgaacgcc aagtaccgcg
    28801 aaacctctcg cggcggcctg gcaatgaaga tcgttgcctg cgattaatcg ctctccagag
    28861 gcctcgtttt gcgaggcctc ttcccgattt ctcatccagc catagcctgt tccagtatcg
    28921 aatgttaccc ttatcgcctg atctttaagg gggttatcgt ggctgttcat ttgcttattg
    28981 tcgatgcact gaatcttatt cgtcgcattc atgccgttca gggttcgccc tgtgtcgaaa
    29041 cctgccagca cgcgctcgat cagctcatta tgcacagcca gccaacccac gctgtcgccg
    29101 tttttgatga tgaaaaccgc agtagcggct ggcgtcatca gcgtttacca gattacaaag
    29161 cgggtcgacc gccaatgccg gaagagttgc acgacgagat gcctgcatta cgcgccgcct
    29221 ttgagcaacg cggcgtcccg tgctggtcaa ccagcggcaa cgaagccgat gacttagccg
    29281 ccacgctggc ggtcaaagtg acacaggccg ggcatcaggc aacgattgtt tcgacagata
    29341 aaggctactg tcagttactt tcaccgacat tacgtattcg tgattacttc cagaaacgtt
    29401 ggctggatgc gccatttatc gataaagaat ttggcgttca accgcagcag ttgcccgatt
    29461 actggggact tgcggggatc agcagttcaa aggtaccggg tgttgcggga atcggaccaa
    29521 aaagcgccac gcagctgctg gtcgagtttc agagtctgga agggatatat gagaatctgg
    29581 atgcggttgc cgaaaagtgg cgcaaaaaat tagaaaccca taaagagatg gcgtttctgt
    29641 gccgcgatat tgcccgctta caaaccgatt tgcatatcga tggcaattta cagcaattgc
    29701 gattggtacg gtaacggcga gccggatacg ccgcaaacgt cgtatccggc attatcacat
    29761 cagcgcattt accaggcggt atggtaaagc tctacaatat cctcaagtgt tgcttcacgc
    29821 gggttgccac cggtacaaac atcatccagt gccgcctgcg ccagtgccgg aatgtcttcc
    29881 ttgcgtacac caacatcacg caaatgtggc ggaataccga catcacggtt gagagcaaac
    29941 accgcttcaa cagcggcatt acgcgcctct tccaggctca taccttccac tttcacaccc
    30001 ataacgcgcg cgatatcgcg gtacttctca ccggtaaagt cagcgttata gcgcatgaca
    30061 tgcggtaaca ggatggcgtt cgcaacaccg tgtggagtgt tgtaaaacgc gcccagtgga
    30121 tgcgccatac catgcaccaa ccctaaccca acattcgaga agcccatacc cgcaacatac
    30181 tgcccgagcg ccatttcttc tccggcatcc ttttcaccag caaccgatcc tcgcagcgcc
    30241 ccagcaatga tttcaatcgc tttaatgtgc agtgcatcgg ttagcgccca cgcgccacgg
    30301 gtaatatacc cctcaatagc atgagtgagc gcatcgacac ccgtcgcagc tttcagcgct
    30361 ggaggcatac catccatcat gtcagcgtca ataaacgcca cctgcgggat atcatgcgga
    30421 tcaacgcaaa caaacttgcg ccgtttttct tcgtcagtga tcacgtagtt aatggtcact
    30481 tctgccgcag tgcctgctgt ggtggggatt gccagaatcg gtacactggg tttattggtc
    30541 ggggaaagtc cttccaggct acgcacatcg gcaaactccg ggttgttgct gataatgcca
    30601 atcgctttac aagtatcctg tggagaacca ccaccaatag cgatcaggta atccgcgccg
    30661 ctattctgga atacaccgag cccttctttg acgacagtaa ttgttgggtt gggcactacg
    30721 ccgtcgtaaa tcgcccatgc cagccctgca gcatccatct tatcggtcac tttcgccacc
    30781 acgccgcatt gcaccagcgt tttatcggtg acgatcagcg ccttctgata accacggcgt
    30841 ttcacctcat cggttaaagc cccaacagca ccccgaccaa accatgccgt ttcgttcaga
    30901 atcattctgt tagccatcat ccttctcctt gttgctttac gaaattactc ttcaatccgt
    30961 aacccatagg ttttgaattt ctccagcact acggcaatct cttcatcgct cagcactggc
    31021 accgggtccg taatcgccag ggtcgtcagg taaagttgcg ccagcacttc aacttcatgc
    31081 gccagccata acgctttttc cagattcacc tcacaagcga taagcccatg atgttgtaac
    31141 aaagttgcct tacgattttt gagagccagc gcaacatgtt cagaaagttc gcgtgttcca
    31201 aaggtcgcat aaggcgcgca aggaatagaa ttaccgccag ccgccgcaat catgtagtga
    31261 atagcgggga tcggtcggtt aagaatggaa actgctgtgc aatgaacggc atgattgtga
    31321 acaaccgcgt tggcatccgg tctgctttga taggccgcca tatggaaacg ccattcgctt
    31381 gaggggagct ttccttcctc atgtttaccg ttgccatcaa taaagacaat atgcgactcc
    31441 gtcagttttt catatggaat gcctgtaggc gtaatcagca tcccatcctg ataacgtaca
    31501 ctgacgttcc ccgctgtccc ctggttcagt cccaggcggg tcatttccag acaagtgtca
    31561 ataatctgac gagcaagttt atttcgttcc attagctacc tctctctgat tcaaaacagg
    31621 gcaataatgt tgttcctttc acactattga attagccgtt taattaccca ccatcttctt
    31681 cctgattaac aagaaagaaa ttcacaagct tatattttgt gacctggttc aactaatcac
    31741 agtaaataac tgcaagttct ctttttataa ccccattaaa aatgaccgct cttaaaaata
    31801 tttatcaaaa
//
LOCUS       DAZFFG010000043        31734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFFG010000043 DAZFFG010000000
VERSION     DAZFFG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.043"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(411..486)
                     /locus_tag="ACHJ7J_002343"
     tRNA            complement(411..486)
                     /locus_tag="ACHJ7J_002343"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Asn,seq:gtt)
     gene            complement(587..2074)
                     /gene="yeeO"
                     /locus_tag="ACHJ7J_002344"
     CDS             complement(587..2074)
                     /gene="yeeO"
                     /locus_tag="ACHJ7J_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO5000571.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAIRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKNAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIILEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            2235..2310
                     /locus_tag="ACHJ7J_002345"
     tRNA            2235..2310
                     /locus_tag="ACHJ7J_002345"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2268..2270,aa:Asn,seq:gtt)
     gene            complement(2348..3298)
                     /gene="cbl"
                     /locus_tag="ACHJ7J_002346"
     CDS             complement(2348..3298)
                     /gene="cbl"
                     /locus_tag="ACHJ7J_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO5000572.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYQQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3400..4317)
                     /gene="nac"
                     /locus_tag="ACHJ7J_002347"
     CDS             complement(3400..4317)
                     /gene="nac"
                     /locus_tag="ACHJ7J_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO5000573.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4643..4718
                     /locus_tag="ACHJ7J_002348"
     tRNA            4643..4718
                     /locus_tag="ACHJ7J_002348"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4676..4678,aa:Asn,seq:gtt)
     gene            complement(4774..5709)
                     /gene="ldtA"
                     /locus_tag="ACHJ7J_002349"
     CDS             complement(4774..5709)
                     /gene="ldtA"
                     /locus_tag="ACHJ7J_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5000574.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANSGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRPMF"
     gene            complement(5771..6850)
                     /gene="cobT"
                     /locus_tag="ACHJ7J_002350"
     CDS             complement(5771..6850)
                     /gene="cobT"
                     /locus_tag="ACHJ7J_002350"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO5000575.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLTGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6862..7605)
                     /gene="cobS"
                     /locus_tag="ACHJ7J_002351"
     CDS             complement(6862..7605)
                     /gene="cobS"
                     /locus_tag="ACHJ7J_002351"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO5000576.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7602..8147)
                     /gene="cobU"
                     /locus_tag="ACHJ7J_002352"
     CDS             complement(7602..8147)
                     /gene="cobU"
                     /locus_tag="ACHJ7J_002352"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO5000577.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDNEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            8334..8504
                     /locus_tag="ACHJ7J_002353"
     CDS             8334..8504
                     /locus_tag="ACHJ7J_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO5000578.1"
                     /translation="MIGVVTKADLASMEEISLVKVWLREAGAHNVLVTSAVNNNGIAE
                     LLALLHTEEGCR"
     gene            complement(8605..8934)
                     /gene="tmaR"
                     /locus_tag="ACHJ7J_002354"
     CDS             complement(8605..8934)
                     /gene="tmaR"
                     /locus_tag="ACHJ7J_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO5000579.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(9106..10164)
                     /gene="yeeA"
                     /locus_tag="ACHJ7J_002355"
     CDS             complement(9106..10164)
                     /gene="yeeA"
                     /locus_tag="ACHJ7J_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5000580.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(10363..10836)
                     /gene="sbmC"
                     /locus_tag="ACHJ7J_002356"
     CDS             complement(10363..10836)
                     /gene="sbmC"
                     /locus_tag="ACHJ7J_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO5000581.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(10955..12121)
                     /gene="dacD"
                     /locus_tag="ACHJ7J_002357"
     CDS             complement(10955..12121)
                     /gene="dacD"
                     /locus_tag="ACHJ7J_002357"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO5000582.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYVEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNIDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            12330..13757
                     /gene="sbcB"
                     /locus_tag="ACHJ7J_002358"
     CDS             12330..13757
                     /gene="sbcB"
                     /locus_tag="ACHJ7J_002358"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO5000583.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFEYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQDYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(13800..14027)
                     /gene="tsuB"
                     /locus_tag="ACHJ7J_002359"
     CDS             complement(13800..14027)
                     /gene="tsuB"
                     /locus_tag="ACHJ7J_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO5000584.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(14041..15099)
                     /gene="tsuA"
                     /locus_tag="ACHJ7J_002360"
     CDS             complement(14041..15099)
                     /gene="tsuA"
                     /locus_tag="ACHJ7J_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO5000585.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSITETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(15278..16636)
                     /gene="plaP"
                     /locus_tag="ACHJ7J_002361"
     CDS             complement(15278..16636)
                     /gene="plaP"
                     /locus_tag="ACHJ7J_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO5000586.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(16626..16688)
                     /gene="yoeI"
                     /locus_tag="ACHJ7J_002362"
     CDS             complement(16626..16688)
                     /gene="yoeI"
                     /locus_tag="ACHJ7J_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO5000587.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(16903..17832)
                     /gene="yeeY"
                     /locus_tag="ACHJ7J_002363"
     CDS             complement(16903..17832)
                     /gene="yeeY"
                     /locus_tag="ACHJ7J_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5000588.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(17878..18702)
                     /gene="yeeZ"
                     /locus_tag="ACHJ7J_002364"
     CDS             complement(17878..18702)
                     /gene="yeeZ"
                     /locus_tag="ACHJ7J_002364"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5000589.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            19128..19178
                     /gene="hisL"
                     /locus_tag="ACHJ7J_002365"
     CDS             19128..19178
                     /gene="hisL"
                     /locus_tag="ACHJ7J_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO5000590.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    19154..19277
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            19324..20223
                     /gene="hisG"
                     /locus_tag="ACHJ7J_002366"
     CDS             19324..20223
                     /gene="hisG"
                     /locus_tag="ACHJ7J_002366"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO5000591.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            20229..21533
                     /gene="hisD"
                     /locus_tag="ACHJ7J_002367"
     CDS             20229..21533
                     /gene="hisD"
                     /locus_tag="ACHJ7J_002367"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO5000592.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDSVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSEELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            21530..22600
                     /gene="hisC"
                     /locus_tag="ACHJ7J_002368"
     CDS             21530..22600
                     /gene="hisC"
                     /locus_tag="ACHJ7J_002368"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO5000593.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLMAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            22600..23667
                     /gene="hisB"
                     /locus_tag="ACHJ7J_002369"
     CDS             22600..23667
                     /gene="hisB"
                     /locus_tag="ACHJ7J_002369"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO5000594.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLSADECD
                     CRKPKVKLVERYLAEQAMDRASSYVIGDRATDIQLAENMGITGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFMLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            23667..24257
                     /gene="hisH"
                     /locus_tag="ACHJ7J_002370"
     CDS             23667..24257
                     /gene="hisH"
                     /locus_tag="ACHJ7J_002370"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO5000595.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            24257..24994
                     /gene="hisA"
                     /locus_tag="ACHJ7J_002371"
     CDS             24257..24994
                     /gene="hisA"
                     /locus_tag="ACHJ7J_002371"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO5000596.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            24976..25752
                     /gene="hisF"
                     /locus_tag="ACHJ7J_002372"
     CDS             24976..25752
                     /gene="hisF"
                     /locus_tag="ACHJ7J_002372"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO5000597.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            25746..26357
                     /gene="hisIE"
                     /locus_tag="ACHJ7J_002373"
     CDS             25746..26357
                     /gene="hisIE"
                     /locus_tag="ACHJ7J_002373"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO5000598.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGEMSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(26454..27431)
                     /gene="wzzB"
                     /locus_tag="ACHJ7J_002374"
     CDS             complement(26454..27431)
                     /gene="wzzB"
                     /locus_tag="ACHJ7J_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO5000599.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(27577..28743)
                     /gene="ugd"
                     /locus_tag="ACHJ7J_002375"
     CDS             complement(27577..28743)
                     /gene="ugd"
                     /locus_tag="ACHJ7J_002375"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO5000600.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRGADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKENSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(28992..30398)
                     /gene="gndA"
                     /locus_tag="ACHJ7J_002376"
     CDS             complement(28992..30398)
                     /gene="gndA"
                     /locus_tag="ACHJ7J_002376"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO5000601.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSDEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAVVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(30564..31520)
                     /locus_tag="ACHJ7J_002377"
                     /pseudo
     CDS             complement(30564..31520)
                     /locus_tag="ACHJ7J_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         8377 a         8234 c         7500 g         7623 t
ORIGIN      
        1 tataatcctt ataaaaaata taatgttcaa ctcgtcatat tgcttataaa aaatcctcgt
       61 tcccagagaa ataatattta ttaaaatggc aggtcttctt tttctgatta ccgaagacaa
      121 agtggcaatt acgtatagtt tcccgataaa gacgcgacag cgacatcccg cataaggcat
      181 ttttctcttt atctttgtac ggtacttcat ggaacagaat ttttgacctt gtgaatcgtg
      241 atgtctgttg gggaggagca atttgctcac tgaagcgtga gactcgatta agcgcacgaa
      301 acacagaaat caaaaaaccc ggccactttt ttttacaaag tgaccgggta aaaataattt
      361 ttatttttta actgccttga gactcataga gatgtctcaa aactaaaatt tggctcctct
      421 gactggactc gaaccagtga catacggatt aacagtccgc cgttctaccg actgaactac
      481 agaggaatcg tgagaacgag gcgaatatta gcgatgccca cccacaatgt caaagcctgt
      541 tttttaaatt tgaaatcgtt tgctgaaata atctgcattt tgtcgtttat tccgacacag
      601 ctggcttttt ttcacacttt tgcggttcgg gtcgagggta tttccacagc caacgtccag
      661 taaccattcg ccagtaaaac agcacggccc gcacagccca gtcggcaaac attcccatcc
      721 agacaccaac cacaccccag ccaagcatga tgcccagcac ataaccgacg acaacccggc
      781 aaccccacat gctcaacata gaaacccaca tagcgtaacg ggcgtcacga gcccccttaa
      841 atccagcggg tagcacccat gaagcggacc aaataggcat aaataaagca tttagccaaa
      901 tcagaatcac aacgacatgt ttaacctgtg gatcctgggt gtaaaacgat gccataaccc
      961 cggcaaaggg agccgttagc caggcgatgg ccgttaatcc aagagtggaa agccagaaca
     1021 catgccgcaa ctgaatctct gcttgcgcta tctgccctac ccccaacctt cggcctgtaa
     1081 tgatcgtaga agcagagccg agcgcacttc cgggtaagtt gataagagcc gcaattgaaa
     1141 acgcgataaa atttccggca ataacactgg tccccatccc ggcaacgaac atttgggtta
     1201 ataaccgacc actggtaaat aacactgatt cgacactcgc tggaatacca atccccatga
     1261 cttccaagat aatgctaaaa ttcagcggtt taaaatagct ctttaacgaa atccttagcg
     1321 caggattaaa accaatcgcc agcacccaca aaattgcaac tgcgccaata taacgagaaa
     1381 tggttaaacc caacccagcc ccgacaaatc ccagtcccgg ccaggagaaa aggccgtaaa
     1441 tcaatatgcc gctaataata atattaagaa tattcaggct accgttaatc agtagcggta
     1501 ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat tagagtgatg gcagctgctg
     1561 gataactgag taccgtcagc tccagataag tcaacgccag tgctttaact tctgtcgtgg
     1621 catcacccgc gacgaaatca ataatttgct cgccaaaatg atgaataagc gttgccaaca
     1681 gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc gctcgtcgtc
     1741 gatcccgctt accgagacta aatgccacaa cgacagtagt accaagatcg atagcagcaa
     1801 aaaaagccat aatgaccata ttgaagctgt ccgccaatcc cacgccggcc atcgcatttt
     1861 ttcccagcca gctgaccaga aaagtgctca gaacccccat caacaggaca caggcattct
     1921 ccatgaagat aggaacagca agcggggtta tctcgcgcca gaacaacact ttgtagctct
     1981 tgcgtatagc gtgccagcga gtgccgtgaa caacctggcg taaagcagag gagatattca
     2041 aagccgacct taattgcaga aagtgaaacc acatttcaaa taatgaggga gaatcagcaa
     2101 agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt gatagaaacg
     2161 gcaaacagtt gggggattta aaaatcgggt ttgacaaaag atttttcgcc gttaagatgt
     2221 gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
     2281 tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt ttatagcggg
     2341 ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc tgccgcttga
     2401 tatcctcaac tgaaagtcct gcattacaaa gttccagaaa gcgccagaca tagttacgct
     2461 gaagttgtcc tcgcttcagt cccaaccaga cagtattagc atcaaaaaga tgccgcgtat
     2521 ccaggcggat taaattcttt tcctcttgtt caccgctgga ttgttcggca actattccga
     2581 tccccagccc aagagcaaca taggttttaa tgacatcaga atcctgcgca cttaatacaa
     2641 tatctgccag caaacctttg cgggcaaatg cgtcatcaat acgtgagcgc cccgtaatcc
     2701 cctgttggta agtgattaac ggccacttcg ctattgactc cagcgtcaat ggtgtaattt
     2761 gcgtcaaggg atgatcgagt ggaacaagca aactatggtg ccaacgaaac cacgggaagg
     2821 cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca gcttcaccat
     2881 tatgcaacaa cgtcgcaatt tcctgtggcg tcccctggat tagctcgagc cgaacctccg
     2941 ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc tgagtatgcg
     3001 tcgttgcaat agtgagaacg ccagacgtat cgttggtaaa caggtctgca agccgacgaa
     3061 cattactggc ttcattcaga atacgttctg caatgaccag taatgctttg cccggttcag
     3121 tcatgcccag cagtcgctta cctcgtcgaa caaatatttc gatgccaagt tcatcctcca
     3181 gttcccgaat atgacggctg acgcctgact gtgaggtaaa aagcatattc gcaacctctg
     3241 tcaggttgta atcctgacgt gcagcttcgc ggattatctt tagttgttgg aaattcacgg
     3301 taaactccgg gcagttcaga tttcccgtta ttgttaaagt ctaatgcccg gcataacaaa
     3361 taataaaaac ccgcatctta ttccatcccg atataacgct tagctcacca attgccactg
     3421 ccttttttcc atcactggag aactaatcac tgacattaac aactctttca ctgcctgtgc
     3481 ctgtggtgac aagttcgctc tggcgggtaa atttaatgac aaagagagac tcatggaagg
     3541 agtggtaatg cgtgacatcc acccgtttac tgcgccacat aacgaacgcg cggccgattc
     3601 aggtaatact gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg caatagactc
     3661 aatttcacca atgacttttg ccgtgagtcg ccgtagggaa aacgcctcat caacacgaag
     3721 tctaacagcg ctgtaatcac tggggagaaa gaggttcatt tgtgcaatag cattaacatc
     3781 aacgctttgc cccgggcaat cttgagttcc taccaggaaa agatcttctt tcagcaatgc
     3841 ctgactggat accccagcca caggggaatg ctcataaatc accgccatat cgagttggtg
     3901 atttatcagt ttttcgttaa gcactgcacc gctattttca tgaagataga taacgatctc
     3961 cgggaattca gcgcgaaccg cctgtaataa gggcatggtg atggatgacg cagcggttcc
     4021 tggcgcaaag ccaatcgaga cttgccccga taatgcctga ccaacgttat gcaccgccag
     4081 ttgggcctgc tcacactgac gtaaaatggc ccgcgcatgg gtatagagaa tttttccggc
     4141 gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagt tgttgattta actcaccttc
     4201 gagtgtggca acctgctggc tgagcgctgg ttgtgcgata tgcaatactt cagcagcctg
     4261 agtcaggcta ccaatatcta caatttttac gaagtatttc aggcgtctga agttcatgtt
     4321 gcctccggtt tttaagaatc ggcccaagtg ccgccattac ttacaaccag attgcaagat
     4381 gcttgccagt tttattttgg cgttgatgta caagctaacc aactgtcaaa taagagatta
     4441 tgatagattc gtcattggtt ctttttatca gcagtcgcgt tcccctgccc cataaaagga
     4501 gagtatgcac cacgatggtt cataaccaat aagattgaaa tctcatcact ttgttgaaat
     4561 tgacagcaaa caaacaaaaa aatgcatttc accctttgac atcaccatgc actgccatta
     4621 atatgcgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     4681 tccgtatgtc actggttcga gtccagtcag aggagccaaa ttcaaaaaag cctgctttct
     4741 agcaggcttt ttgctttcta attaccaacg ctcttaaaac atcggtcttg aaccagaact
     4801 aatttgcaca ggcattcccg atcgtcgttg caacgcagca tttgcgcgat tcacatcaac
     4861 ttcttgcccg ttgataaacg cccgcaaaga tggggttacc ggcaatggca cttttcggtc
     4921 agactcatat tctgcacgat tgcgcgacaa cggctcatga acttccagcc agttcgagcc
     4981 atctggttca gtggtgtatt ttactggctg gtcgataatt tgcacacgcg tcccaacagg
     5041 aacattatca aacagatatt tgatatcgtc attgcgcaga cgaatacagc cctgacttac
     5101 ccgaagtcca ataccaaaat tggcattggt accgtggatg gcatacaacc tgccaatata
     5161 aatcgcgtac agccccatgg gattatcggg ccccgcagga acaaatgcgg gcaaactctc
     5221 ccctcgtttc gcatattcgc gccgagtgtt cggcgttggc gtccaggttg gagcttcttg
     5281 tttacgttca acggtagtca cccagttacg cggggtttct cgcccagcct ggccgatacc
     5341 aataggaaag acttccacag tattactgtc tggtgggtag taataaagac gcatctcagc
     5401 gacgttaacc acaatccctt tacgaacagt ggcgggcaaa atcagttgct gcggaatggt
     5461 gagttgcgag ccagacttcg gcaaaaaaac atcagcgccc gagttcgctt caagcatgtt
     5521 acttaaccct tgcccgtatt gtgcggcaaa agtctccagc ggctgggtat tgtgatcagg
     5581 aacagttaca gtaaacgact gccccactaa acggctaccc tctggaggta atggataagt
     5641 taccgccagg ctagtatggc tggcaaaaag cagagcaaat gagcaaagaa tatttacacg
     5701 acgcatcatg tccctttcct aagtcgcgaa agctatccgt taagtatagc ttttatcaga
     5761 cttttcgttt ttaactgttc aaatcagaag tcgtattccc cggtagaaca atattactgg
     5821 cagcaagttc gcccatgttg ttatatatcg cacaggcagc ttcgatgatg ggcatcgcca
     5881 gagctgcgcc actcccctca cctaaacgca tctccatatt gagataaggc tccagcccca
     5941 aatgcgacag cgctatacgc gccccttttt ctgccgacag gtgagaagga atgagatacg
     6001 gtttgattgc aggagacatc tggcaggctg cgagcgcagc agcataagaa agaaatccat
     6061 ccagcaacac gggtaaaccg caggaagcag cgcctaacat cacgccagcc attccgacca
     6121 aatcaaatcc acccactttt gccagaacat caacgccatc ctgcggattt ggttgattca
     6181 acgtaatcgc ccggcgcaca acatcaattt tattagccag tttatctgtc ggcaggtttg
     6241 cgccaatccc aaccacttct tcaggatccc ggccagtgat tgtgctgact attgctgctg
     6301 ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa cagcgtgacc ccgttttttg
     6361 ccagctcccg cgtataacat atgacgtcca aaagcaactt ttcagcctga cggcgactca
     6421 ttgccggagc tgaagcaata ttgccgctac ctcgtgcgac acgcatgttg ataagcccgg
     6481 ggataggctc agcagtatca ataccaacat caattacgtg gacgttagcg cccgcttgtg
     6541 ctgccagcac acacacgccg gttgttccgc gggtcatatt ttcagcctga atggctgtca
     6601 cttcttttgg ggaaatagcg accccttcct cccagacgcc gtgatcggca cacataacca
     6661 gtaccgcttt tttgcccaca tgcggtatgc cagtcaaccc cggcattcct gccagttgta
     6721 tggcaagcgc ctccagcctt cccaggctac caacaggttt gagtaacccg tcaatatgcc
     6781 gttgtgcacg cgacatagcg gcaggatcga tagcagggat cgtattcagt aaatcggcaa
     6841 gtgtttgcat gtcacgtctc tttatagcag tgccagcagg aagaccagtt caccaagctc
     6901 tatagcagct cccagcgtat cgccggtttg cccgcccagc gtacgtttga gaagctgacc
     6961 gaggatgaaa atcgccacca tcgtcaccac catagcagcc acaccatgca tacccggcaa
     7021 taatacggca gcaaaaatag cggctaagcc gagtgtgacg caggtttgtc gcccatcaat
     7081 tttgccaatg aacacattgc caagcccttc ttcccgggca taacgatgac gatacatcaa
     7141 taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc
     7201 gccacgcaga gccaactcgc ttaacaccag aatctttgcc agtacaacaa aaatcaatgc
     7261 cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc gatcgcggct
     7321 acgtgcagaa aatacgccat cacaggtatc ggcgaggccg tccaggtgga atccccctgt
     7381 catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtacgccac accatgcctg
     7441 cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatta aaggaaaagt
     7501 aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac gcggcacagg
     7561 cagacgcgta ataaaagaga gcatcgccca aaataattta ctcatttgat ttttactcca
     7621 atacccgaaa ccaccagcca cacttcattt gccgcagcgg ccagctgctg atttacccgc
     7681 ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact
     7741 tcgttagtca ctaatacaac ctttgcgggg caacgttggc aggcagcaat caacgactga
     7801 atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg
     7861 ccgccataat caaacaacag attagtcacc attgtggtaa cgcattccag caacacagct
     7921 tcatgcgggt taatgtctgc atgaattaat tcatcaagat gttgccagcg ctccaccgtg
     7981 cgccagtgcg ccgggcggcc ttgccgatga tgttctatcc gtgcagccat ctcattatca
     8041 aggatttgag aggtggcgat atacagaacc tgtgaagagt ccccaataag cgcctctgcg
     8101 tggcgactct tcccgctccg tgcgcccccc gtcaccaaaa tcatcatacc gactcctgat
     8161 tcaattgtat gatggtattc attgaacaga acaatggatc cccaccgttg ctgaccgcaa
     8221 aacatttacg cgattaatac tgcgcgtaat acaattaaaa atccagcatt ctcaatccat
     8281 tacgaaagaa gatggcattg cgggatagcc agacttcctc gtgttaacgt ccgatcattg
     8341 gagtagtcac caaagcagat ttagccagca tggaagaaat atctttggtg aaagtttggt
     8401 tacgggaagc tggagcgcac aacgtgttag taaccagtgc ggttaataac aatggaattg
     8461 cagaactgtt agccttgctg catacagaag aaggctgtcg ctaatttcga caggttctca
     8521 gatgagaatg atgacggtga tgcacagaaa agaatcaatg agcaatgagg gttgccgggc
     8581 aaccctcatt gaataaaaca ggaattactt cgcttcgccg tttttcattt cgcccatagc
     8641 tttcagcttt ttggagatat cgcggcgttc tttggagagc tcggcatttt tgatgatgta
     8701 atcgtcaaca cgatcttcat agtcaccttt catgctggcg atgatgccct ggattgcttc
     8761 aacgctcatc cccggcttga tgtaatcgct caggttgtcc agcagcagga cgcgcttctg
     8821 gttgtcacgg atctttttct caacgtcctg aatttcacgt tgcagtttgt tcttacgacg
     8881 gaacagacga acaaattcca gtacgtcctg gaatgaaggc ttggtagttt ccatttttat
     8941 acccctgata atgtgagagt cggattcgtt taagcaaccg ctattcgtta gggccaacat
     9001 tactcatagc tgccgcagat gacaatgctt ttatcccttt tcactatcat accctttatc
     9061 cttgctgaat cgaagcagca gcaagatgat tctgaagttc aggaattatt tgcgcaaggc
     9121 ccggcaaatc agattcgata gcaactcaag ctgatgtgcc gtttccatgt tcagccacac
     9181 ataaccatag attggtgttt ccacaacctt caggtcatgg tggttattga gcaactgacg
     9241 cagctcttcc acagcatcgt tcaatttttc cgtattggca aaaaccggct gcggattacc
     9301 ttcgtacagc gcatgaacaa ggctcagcag tatttgctgc atcatgtgct gggtatcacg
     9361 aagtttttgc gcgttcaata acacgaaatg gctggggcgc gtggcccagt acgcattgat
     9421 ttgcaactcc agcatacaaa ccagattgcg attaattgtc tggatacctt catatatcga
     9481 ttttggaata cgggtttctt tgctggcggg cgcaatcaac ccacgcattt tcacggcatc
     9541 ggtcaggagt ttttgtagat ggctttccag acgtgggcgt tcgagtaagt tcggtgagaa
     9601 tgcagattga tagacccgat tatactcggt cagacttttc gccagttgaa tacgccagtg
     9661 gatgaacgcc cgttgtggcc agataccggt aaacaacatt gccagtaaag agccgaggat
     9721 cacatcgccg cttcgccata acgccgtatc aatttcacct gtaggggaac ccacaacaat
     9781 tgccagcgtc accccaatca ataatccttg atacggtttc ttgcccagcg ccagccaacc
     9841 gcaaagaaac atagccgccg cgcaccagac taacatcagc ggtaacgaga ttaactccag
     9901 ttgcagagcg ataagaccta atatcgaacc caacaccgta ccgccaatac gttcgaaggc
     9961 gcgagggaca acgttacccc agaacgagat tggccccata atcaccacca tggtgaccag
    10021 cggccaggtg ctttccggga tagtaaacag gcggataatg agaaaagtga gcaggaatgc
    10081 cagcgcgacc cgagtaccat gcacaatgcg gtaatggcgg tatacccgga tttcaaacgg
    10141 gcttaatgac ttatcggcac gcacgcagaa actccgtaat gaagattaaa agcgctcata
    10201 actcatctgt cggccagaag gcgcgcctct gaattatccg acggacaaac cgggaacagt
    10261 gtaatttccc ggcaggagcg aaaaaattga aaggcgcatc ttactctttt cgctttcaaa
    10321 aaaaagagtg gtcatcgcgt taacacaccg ccctgagatg aattagtgat gttttggctg
    10381 cacagcaaca tacatttcga tatcccagta cccatcttcc gcgccattgt tcaaataaac
    10441 ctcgaagcag ggctttggta acatttcata agcactgtcc tgcaagaggc tattaaagaa
    10501 ctgataccag ggtttagcaa aatcatcacc gactacacga gctaccgcca ccgcatactg
    10561 accacctgaa atttctgtca gaatgacgcc ctcactgttt tcgggaagcg taaagtaacc
    10621 cggcaccgtc acgacggtgt cgcagcgtaa tttttcggcg ggtgtttcat ctggattgtc
    10681 gtaatagaca gcaacccact ccttcggcac aatattttta ctatctaccc acatcatcaa
    10741 ctgctcaaaa cctttcttta ccgtctgttc ccacgggcca acgagatgga aacctgcaac
    10801 ggtacgttta tcttcctgct taatctcgta gttcatgacg cctccattaa cactgttttt
    10861 atatacagta tagttgcaaa attaaaacca caaggaatga gtgttgatta tgcgagcaga
    10921 ctcgcactcc tgccagtctg ctgcaaaaga aaggtcaggc cttatggtgg aaataatcac
    10981 tcaggcggga aaacataccg ccttccccga cagattccag ggtaaccagc ggccagtgcg
    11041 ccacctgttt atcacggtcg taaagttcaa tttcccctac ccgctgatgg gcgctaattg
    11101 gcgcggtgag ttctttacca tcaagggtat atttggcttt gatatgtgga atttcggctt
    11161 tcggtagcac catccagaac tcttgttccg ttcccaggtc gatattttct ttatcgccat
    11221 accagatgcg ttccgttccg acctttttcc cacggtgcaa aatttgcacc gtagtaaagt
    11281 tttgttgccc ccaacgtagt aattttcttg cctcttcctc acgacctttt gcactgtcag
    11341 cacccattac cactgcaatg agacgacgct gcccatctac agccgaagca atgagattaa
    11401 acccggcacc agaagtatga cccgttttca ggccgtcaat attcatggtt ttatcccaca
    11461 ataacccgtt acggttttgc tgggtgatac cgttccaggt gaggcttttc tcactgtaca
    11521 tatgataaaa ctcgggctcg ccgtggatga tagcgcgaga aagcacagct aaatcataag
    11581 ccgagctatg ctgacctggc gcatccaggc catgcactgt ttcaaaatgc gtatccttga
    11641 gatgcagctt ctcgacatag ttgttcatca tttcaacaaa ctgccgttgc ccaccggcaa
    11701 tatagtcagc cagtgcaaca caagcgtcat ttccggaatc cacaattaaa ccacggctta
    11761 aatcacgtac cgatacgcga tcgccctctt tcaaaaacat cagtgaagaa ccgacaaaca
    11821 ccggattatc tttcgcccac gcatcgcgcc ccacggtgac aatatcgtct ggcgtaatgc
    11881 gatgactatc gatagcgcga tccacgacat aacccgtcat cagctttgtc aggctggcgg
    11941 gattgcgctg ttgatgctca ttacccgcgg tgaggatctg tccggtggtg taatccatca
    12001 gtacccagga cccggcatga atctctggag gctgaggtga aaaaggaatg ttttccgccg
    12061 caaaaccaga cgataagtta aaaacgaaca aagaagcagc aataataaga cggcgtttca
    12121 acagcaaacc ctcaggagtt tcaaatagct gttcttttta cggaaatact tatgaactgg
    12181 ctggaataaa gtgcaagaaa atgtgactac cctctcattt tcatctgaca tgatctgttg
    12241 ccactcgctg tcaaattgtc gcgctaaagc tgattagcac ggtgatattt gatactctgg
    12301 cagacagcag aaataacgga tttaacctaa tgatgaatga cggtaagcaa caatctacct
    12361 ttttgtttca cgattacgaa acctttggca cgcaccccgc gttagatcgc cctgcacagt
    12421 tcgcagccat tcgcaccgat agcgaattca atgtcatcgg cgaacccgaa gtcttttact
    12481 gcaagcccgc ggatgactat ttaccccagc ctggagccgt attaattacc ggtattaccc
    12541 cgcaggaagc acgggcgaaa ggagaaaacg aagccgcgtt tgccgcccgt attcactcgc
    12601 tttttaccgt accgaagacc tgtattctgg gctacaacaa tgtgcgtttc gacgacgaag
    12661 tcacacgcaa cgttttttat cgtaatttct acgatcctta cgcctggagc tggcagcatg
    12721 ataactcgcg ctgggattta ctggatgtta tgcgtgcctg ttatgccctg cgcccggaag
    12781 gaataaactg gcctgaaaat gatgacggtc taccgagctt tcgccttgag catttaacca
    12841 aagcgaatgg aattgaacat agcaacgccc acgatgcgat ggctgatgtg tacgccacta
    12901 ttgcgatggc gaaactggta aaaacgcgtc agccacgtct gtttgaatat ctctttaccc
    12961 atcgtaataa acacaaactg atggcgttga ttgatgttcc gcagatgaaa cccctggttc
    13021 acgtttccgg aatgtttggg gcatggcgcg gcaataccag ctgggtggca ccgctggcgt
    13081 ggcatcctga aaatcgcaat gccgtaatta tggtggattt ggcaggagac atttcgccat
    13141 tactggaact ggatagcgac acattgcgcg agcgtttata taccgcaaaa accgatcttg
    13201 gcgataacgc cgccgttccg gttaagctgg tgcatatcaa taaatgtccg gtgctggccc
    13261 aggcgaatac gctacgcccg gaagatgccg accgactggg aattaatcgt cagcattgcc
    13321 tcgataacct gaaaattctg cgtgaaaatc cgcaagtgcg cgaaaaagtg gtggcgatat
    13381 tcgcggaagc cgaaccgttt acgccttcag ataacgtgga tgcacagctt tataacggct
    13441 ttttcagtga cgccgatcgt gcagcaatga aaattgtgct ggaaaccgaa ccgcgtaatt
    13501 taccggcact ggatatcact tttgttgata aacggattga aaagctgttg ttcaattatc
    13561 gggcacgcaa cttcccaggg acgctggatt atgccgagca gcaacgctgg ctggagcacc
    13621 gccgccaggt attcacaccc gagtttttac aagactatgc agaagagatt cagatgctgg
    13681 cgcagcagta tgccgacgat aaagaaaaag tggcgctgtt aaaagcgctt tggcagtacg
    13741 cggaagagat cgtctagttt gaagcctccg ccgctgatac ggcggaggac aatgttgggt
    13801 caggcttttt gaacggtgat gctccacgca gcatcgccaa tctgctgata atcggtgatg
    13861 gcatgtcctt cttctgccgc ccactgtggg attgcttcag ttgcctgggt gcaatcaaac
    13921 tcaattacca actcatcgcc gctggccatt tctgccagtg ctgcttttgc ttctatgagt
    13981 ggaaacggac agacctgcgt caccacatcc agctttttaa tcgccatctt tacaccttac
    14041 ttaatttgcc gcagcagttg ccagtcgcgc cttacgctgc ggtcgaacat agacaagcca
    14101 ggacgcagtc cagactccga gaatcataaa tacaaggcca acccagccct gccaggtcat
    14161 cattgcagtc ataaccagtc cattaccgat agagcaacca cctgcaatac tggcaccgaa
    14221 gcccatcagt acaccaccta acccgctacg taatgttgtt tgtgcatcag ctgcgcgaac
    14281 gcggaactca cggctcgctt tggctgcaat aaatgacccc acgaagatcc ctaacaccag
    14341 aaaaacgccc cagttaatgt atttcacgtc acccgcgacc agaaattgca gaatattggc
    14401 cgttggggaa gtgattccta acccaaacat gcgtccggtt gcttcactca gtggccaggc
    14461 taaaagcgca atcaaaccga tgagtacagc agtgacaaag ggatgccagc gtttttcaaa
    14521 cagaatatga gcgatcccgg ttcggcgcgg cggtaaggtc gcgactttga gttttggctt
    14581 cttcaactct ttcatcacca cccagagcgt tatcaccagc aaaacggtaa ccaacggcca
    14641 cacagacaaa ttaaaagtct cagtaataga gttatgttca gtactgtagt gctgcaaggt
    14701 ttgatttaaa ccactggcat gtggagaacg catcaccgca ctcatcacca tataagtgaa
    14761 aagcgcgatc caactgccga tcaatccctc acccgcgcga taccaggtcc cggtggcaca
    14821 tccacccgcc agaacaattc ccagcccgaa gatataccca cctataacag taccaagcca
    14881 cgggaacgcc ccggcttcgt aagtcagtag gcccgcctga attaacgcaa aaacccccac
    14941 gctttgtacc gaaattgcaa tcagcaaggc gtaaaacatg cgattatttt tcgcgatata
    15001 catatcgcga aaaccacctg tcaggcaaaa acgcccacgc tgcatgacaa atcccagcag
    15061 agcaccacaa attagcccgc ttaatatcat tgaaaacata aattaattaa ccagatgaat
    15121 gttaatgagg aaattattca tgactggtgg aatgcagacc aataaccaaa ttctctaaat
    15181 tagaacaaat ggttattaat gaggatttcg ataaaaaaga ataaaaaaac cggagccaaa
    15241 tgttcattcg actccggttt attattagaa aagatgatta tgctacgtct tcgtactgcg
    15301 gaaccgggtt acggaagctc ttggtgacgc aagccaggta aatcagaccg atagcagccc
    15361 agatcagacc cagaaccatt gagctttctt ccaggttaac ccacagtgca ccaacggtca
    15421 gcgcaccaca catcggcagg aacagatact ggaagtgatc tttcagcgtc ttattacgct
    15481 tctcacggat ccagaactgc gagataaccg acaggttaac gaaggtgaac gccaccaacg
    15541 caccaaagtt aatcagcgcc gtagccatta ccaggtcgaa gttgattgcc agcaaggcaa
    15601 tcgcgccaac caggatgatg ttcatcgccg gagtacgcca tttcgggtgt acataaccga
    15661 agaagctttt cgggaatacg ccgtcacgac ccattacgta catcagacgc gctacgcctg
    15721 catgcgccgc cataccggac gccagtacgg taatggtgga gaagatcagc gcgccaacct
    15781 ggaacgcttt acccgcaacg tacagcatga tttcaggctg tgacgcatcc ggatctttaa
    15841 agcgagagat atccgggaag tacagctgca ggaagtaagt tgcaaagatg aagatcatgc
    15901 cgccgatcag cgcggtcagg aaaatcgcac gcgggatcac gcgctctgca tctttggttt
    15961 cttccgacag gttgctgatg ccgtcaaagc cggtaaagga gaagcacagg attgtcgccc
    16021 cggtaatcat cgggataaca tgcgcatcgc cagaccagaa cggacgagtg ctcgccagcg
    16081 taccagcgcc ttcaccttca aatacgccat aaacaaccat gcccagaatc accgcgatca
    16141 gcactacctg caacacgacg attacagtgt tgaagttcgc tacggatttc agactacgca
    16201 ggttaaaggc ggtcataaag gccaccagcg ccaccacaaa catccacgat gggatggaag
    16261 gcaccagagc ttcaaaatag attttcgcca gcagaatgtt gatcatcggc gcgaacagat
    16321 agtcgagcag agaagaccaa cccaccataa agccgacagt cgggctaatg gatttctggg
    16381 cgtaagtata tgcagagcca gcagaaggat agcggcgaac cagcttcccg tagctcagag
    16441 ccgtaaacag gatcgcaatc aatgcgaacg cataggctgt cggcacatga ccatccgtga
    16501 ggcctgaaac gataccaaaa gtatcaaaca gcgtcatcgg ctgcatatag gcaagaccca
    16561 tcattacaac cggaactaac gtaagcgttt tacgcaattc cacgcgagag gtgtttggag
    16621 taacgttatg cgacatggtc attctccttt acggtgataa ccgtcgcgta agcaaaaaat
    16681 tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta aatatctatc
    16741 cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa taaccgacgc
    16801 tgatgaaacg tcggttttta gtcatttttt gacagcggcg cattgtgcct aaatgggggg
    16861 gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttaaatgg ctgattttcc
    16921 gtcatcagat tttgcataaa caccggcgat cgcactattt gctaaaattt catcccgcca
    16981 ccagccgctg gcaagccctg cggtctgttc attccagcca atccagacgg gttcaaagag
    17041 agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac gttgcgccag
    17101 ataacggggt aaatagccgc agccgaggcc gctaatttgc agctccagct tggttttaaa
    17161 atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag aagcgcctgc
    17221 ctgcgcagtg tcccccacca caatcgcccg atatcgctta atgatacgac ggtttaacgg
    17281 ctcttcttcc tgggctagcg gatgatgagg cgcgaccgca aagacttgtt ccagatcgcc
    17341 caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc caacaataat
    17401 atccgctcgc ccctgagtca gggcatccca ggaaccaccg agtacgccat tgataaactt
    17461 caggcgcgtc acgctatgat gttgataaaa agcttcaatc agtggcgcaa gcagagaaaa
    17521 aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat gcagtttgat
    17581 cgcctgcttt tccagttctc gcacggtatg cagaacttcc cgccccttct ctaataacat
    17641 ttttccggta cgggtgaatt tagcgcggtg accgctacga tcaagtaatt gaatattaag
    17701 gtcgctttcc agcttatgaa cggtgtaact cagagcggac ggcgtcttgt agagtttggc
    17761 cgacgccgcc gcaaaactgc cttctttttc taaggcatcg aggatcatca acacatccag
    17821 cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg tgatcgttta
    17881 ctccagcggc attaccagcg gatcgggata ctggtattca aatcccagtt cattacaaat
    17941 ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga actgcggagg
    18001 ttccagcccc agtaaacggg ccatctgcgg atagaaaaca ttacgcgcag ggtgagctgg
    18061 cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacaggg tgatagcgcc
    18121 aataacatct tctaaatgga ctaaattaac accatgttca ccatcaggcg cggtttttcc
    18181 ggcaaagaag cgtccgggat gacgtcccgg tccgaccagg cccgcaagac gcagaatatc
    18241 gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata ctcgtccact
    18301 gttggttacc ggattacgcg gtgtagtttc tttcaccgtg ccttgcgcgt cgccatagac
    18361 agacgttgag ctggtaaaaa taatgcgggg aatacgatgg gccagcgcgc tatccactaa
    18421 ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacgtg ccggaagcgt
    18481 aatgaccagc gcgtcggcat ccatcagggc atccagatcg tcagaatcgc aaactaactc
    18541 aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt cgacaccatc
    18601 ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca tcgccagcgg
    18661 catgcctaac caccctaacc cgacaattgc gacctttttc atccgttatc tcctgataca
    18721 ccttagatct ataaggctac gctagcgtat caaaactgac aattcattct atgaatgaaa
    18781 tcgaaactgt tagcaaatat accattgcct gatgcgctcc gcttattagt cctacaaaac
    18841 atcaatatat tgaatttagg caacatttgt agaccagata aggcattcac gccgcatccg
    18901 gcaggaacaa agcccacttt agttacagaa cacaatgttc ttgagggttt taaagacata
    18961 ttttcacgat tacttctcta acgtagtcac tccaggcccc cacctttccc cgctcattca
    19021 ttaaacaaat ccattgccat aaaatatata aaaaagccct tgctttctaa cgtgaaagtg
    19081 gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg acacgcgttc
    19141 aatttaaaca ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg
    19201 aagacattca gatcttccag tggtgcatga acgcatgaga aagcccccgg aagatcacct
    19261 tccgggggct tttttattgc gcggttgata acggttcaga caggtttaaa gaggaataag
    19321 aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg tttaagtgat
    19381 gactcacgtg aattgctggc gcgctgtggc attaaaatca atcttcacac ccagcgcctg
    19441 atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc
    19501 ggtctggtaa tggatggcgt ggtagacctt gggattatcg gcgaaaacgt gctggaagaa
    19561 gaactgctta accgccgcgc ccagggtgaa gatccgcgct actttaccct gcgtcgtctg
    19621 gatttcggcg gctgccgact ttcactggca acgccggttg atgaagcctg ggatggccca
    19681 ctctccttaa acggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc
    19741 gaccagaaag gcatctcttt taaatcctgc ttactgaacg gttctgttga agttgccccg
    19801 cgtgccggac tggcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagca
    19861 aacggcctgc gcgaagtcga agttatctac cgctcgaaag cctgcctgat acaacgcgat
    19921 ggcgaaatgg aagaatccaa acagcaactg atcgacaaac tgctgacccg tattcagggt
    19981 gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccga acgtctggat
    20041 gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacta ttctaccgct ggcgggtgat
    20101 caacagcgcg tagcgatgca catggtcagt agcgaaaccc tgttctggga aacgatggaa
    20161 aaactgaaag cgctgggtgc cagttcaatc ctggtcctgc cgattgagaa gatgatggag
    20221 tgatcgccat gagctttaac acaatcattg actggaatag ctgtactgcg gagcaacaac
    20281 gccagctgtt aatgcgcccg gcgatctccg cttctgaaag cattacccgc actgttaacg
    20341 atattctcga tagcgtgaaa acgcgtggcg atgaggccct gcgggaatac agcgcgaagt
    20401 ttgataaaac cacggttacc gcactgaagg tgtctgctga ggaaattgcc gccgccagcg
    20461 aacgtctgag cgaggagcta aaacaggcga tggcggtggc agtaaagaat attgaaacct
    20521 tccacacagc gcaaaaactg ccaccggtag atgtagaaac tcagccaggc gtacgttgcc
    20581 agcaggtcac gcgcccggta gcttcagttg gtttgtatat tcctggcggc tccgccccgc
    20641 tcttctcaac ggtattaatg ctggcaactc cggcgcgtat tgcgggctgt aaaaaagtgg
    20701 tgctgtgctc accgccgccg attgccgatg agatccttta tgcggcgcag ttgtgcggtg
    20761 tacaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcgctggcg tttggtacag
    20821 aatccgtgcc gaaagtggac aaaatcttcg ggccaggtaa cgcctttgtc accgaagcga
    20881 aacgtcaggt gagccagcgt ctggacggtg cggcgatcga tatgcccgca ggcccgtcgg
    20941 aagtgttggt gattgctgac agcggcgcta cgccggattt cgtggcttct gatttgcttt
    21001 ctcaggctga acacggcccg gactctcagg tgattttact gacgcccgat gctgatatgg
    21061 cgcatcaagt tgccgaagct gtcgaacgcc aactggcaga actgccgcgt gccgaaaccg
    21121 cccgccaggc actaaacgcc agccgcctga tcgtgactaa agatttagcg cagtgcgtag
    21181 agatctccaa ccagtacggc ccggagcacc tgatcatcca gacccgcaac gcccgcgatc
    21241 tggtcgatgg catcaccagc gccggttcgg tatttcttgg tgactggtca ccggaatcgg
    21301 caggtgatta cgcctccggc accaaccacg ttctaccgac ttacggttac accgccacct
    21361 gttccagcct cgggctggca gatttccaga agcgtatgac cgtgcaggaa ctgtcgaaag
    21421 aaggtttctc cgcgctggct tcgaccattg aaacattggc cgccgccgag cgcctgaccg
    21481 cccacaaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca tgagcaccgt
    21541 gactattacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc agtcggcgcg
    21601 tcgtctgggc ggtaatggcg acgtctggct gaacgccaac gaatatccca cagccgtgga
    21661 gtttcagctt actcagcaaa cgctcaaccg ctacccggaa tgtcagccga aagcagtgat
    21721 tgaaaattac gcgcaatatg caggcgtaaa accggaacag gtgctggtca gccgtggcgc
    21781 ggacgaaggt attgaactac tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct
    21841 ctactgcccg ccaacgtacg gcatgtacag cgtcagcgct gaaaccattg gcgtcgagtg
    21901 ccgcacagtg ccgacgctgg acaactggca actggactta cagggcattt ccgacaagct
    21961 ggacggcgta aaagtggtct atgtttgcag ccccaacaac ccaaccgggc aactgatcaa
    22021 tccgcaggat tttcgcactc tgctggagtt aacccgcggt aaagcgattg tggttgccga
    22081 tgaagcctat atcgagtttt gcccgcaggc atcgctggct ggctggctgg cggaatatcc
    22141 gcacctggct attttacgca cactgtcgaa agcttttgct ctggcggggc ttcgttgcgg
    22201 atttacgctg gcaaacgaag aagtcatcaa cctgctgatg aaagtgatcg ccccctaccc
    22261 gctctcgacg ccggttgccg acattgcagc ccaggcgtta agcccgcagg ggatcgtcgc
    22321 catgcgcgaa cgagtggcgc aaattattgc tgaacgcgaa tacctgatgg ccgcactgaa
    22381 agagatcccc tgcgtggagc aggtttttga ctctgaaacc aactacattc tggcgcgctt
    22441 taaagcctcc agtgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca
    22501 gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag
    22561 ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa gtatcttttt
    22621 atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt ggaccgtttt
    22681 gacaaactcg cctttgaacc gggcgtgatc ccggaactgc tgaagctgca aaaagcgggc
    22741 tacaagctgg tgatgatcac taatcaggat ggtctgggaa cacaaagttt cccgcaggcg
    22801 gattttgatg gcccgcacaa cctgatgatg cagatcttca cctcgcaagg cgtgcagttt
    22861 gatgaagtgc tgatttgtcc gcacctgtcc gccgatgagt gcgactgccg taaaccgaaa
    22921 gtaaaactgg tggagcgtta tctggctgag caagcgatgg atcgtgccag tagttatgtg
    22981 attggcgatc gcgcgaccga cattcaactg gctgaaaaca tgggcattac tggtttacgc
    23041 tacgaccgcg aaatcctgaa ctggccgatg attggcgagc aactcactaa acgagaccgt
    23101 tacgcccatg tagtgcgcaa caccaaagag acgcaaattg acgtccaggt gtggctggat
    23161 cgcgaaggtg gcagcaagat taacaccggt gttggcttct ttgatcacat gctggatcag
    23221 atcgccaccc acggcggttt ccgcatggaa atcaacgtca aaggcgacct ctatatcgac
    23281 gatcaccaca ccgtcgaaga taccggcctg gcgctgggcg aagcgttaaa aatggctctt
    23341 ggcgacaaac gcggtatttg ccgctttggt tttatgctgc cgatggacga atgccttgcc
    23401 cgctgtgcac tggatatctc tggtcgcccg cacctggaat ataaagccga gtttacctac
    23461 cagcgggttg gcgatctcag caccgagatg atcgagcact tcttccgttc gctctcttac
    23521 accatgggcg tgacgttaca cctgaaaacc aaaggtaaaa acgatcacca ccgtgtagag
    23581 agcctgttca aagcctttgg tcgcaccctg cgccaggcca tccgcgtgga aggcgatacc
    23641 ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatacc ggctgcgcca
    23701 acctgaactc ggtgaagtct gccattgcgc gccacggtta tgaacccaaa gtcagccgtg
    23761 acccggacgt cgtgttgctg gccgataaac tatttttacc cggcgttggc actgcacaag
    23821 cagcgatgga tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc tgtacccaac
    23881 cggtgctggg catctgctta gggatgcaac tgctggggcg gcgcagcgaa gagagcaacg
    23941 gcgtcgactt gctgggcatc attgacgaag acgtgccgaa aatgaccgac tttggtctgc
    24001 cgctgccgca tatgggctgg aaccgcgttt acccgcaggc aggcaaccgc ctgtttcagg
    24061 ggattgaaga tggcgcgtac ttttactttg ttcacagcta cgcaatgcca gtcaatccgt
    24121 ggaccatcgc ccagtgtaat tacggcgaac cgttcactgc ggcggtacaa aaagataact
    24181 tctacggcgt gcagttccac ccggagcgtt ctggtgccgc tggcgctaag ttgctgaaaa
    24241 acttcctgga gatgtgatga ttattccggc attagattta atcgacggca ccgtggtgcg
    24301 tctccatcag ggcgattacg gcaaacagcg cgattacggt aacgacccgc tgccgcgctt
    24361 acaggattac gcagcgcagg gtgccgaagt gctgcatctg gtggatctga ccggagcaaa
    24421 agatccggca aaacgtcaaa tcccgctgat taaaaccctg gtcgcgggcg ttaacgttcc
    24481 ggtgcaggtt ggcggcggcg tgcgtagcga agaagacgtg gcggcgttac tggaagcggg
    24541 cgttgcgcgc gtggtggttg gctccaccgc ggtgaaatca ccagaaatgg tgaaaggctg
    24601 gtttgaacgt ttcggtgccg atgccttagt actggcgctg gatgtccgta ttgacgagca
    24661 aggtaacaag caggtggcgg tgagcggctg gcaagagaac tcgggcgttt cactggaaca
    24721 actggtggaa acctatctgc ccgtcggcct gaaacatgtg ctttgtaccg atatctcgcg
    24781 cgacggcacg ctggcaggtt ctaacgtctc tttatatgaa gaagtgtgcg ccagatatcc
    24841 gcaggtggca tttcagtcct ccggcggcat tggcgacatt aacgatgttg ctgccctgcg
    24901 tggcactggc gtgcgcggcg taatagttgg tcgggcatta ctggaaggta aattcaccgt
    24961 gaaggaggcc atcgcatgct ggcaaaacgc ataatcccat gtctcgacgt tcgtgatggt
    25021 caggtggtga aaggcgtaca gtttcgcaac catgaaatca ttggcgatat cgtgccgctg
    25081 gcaaaacgct acgctgaaga aggtgcagac gaactggtgt tctacgatat caccgcttcc
    25141 agcgatggcc gtgtggtaga taaaagctgg gtatctcgcg tggcggaagt gatcgacatt
    25201 ccgttctgtg tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat tctttccttt
    25261 ggcgcggata aaatttccat caactcccct gcgctggcag acccaacatt aattactcgc
    25321 ctagcggatc gttttggcgt gcagtgtatt gtggtgggta ttgatacctg gtacgacgct
    25381 gaaaccggta aatatcatgt gaatcaatat actggcgatg aaagccgcac ccgcgtcact
    25441 cagtgggaaa cgctcgactg ggtagaggaa gtgcaaaaac gcggtgccgg agaaatcgtc
    25501 ctcaacatga tgaatcagga cggcgtgcgt aacggttacg acctcgaaca gctgaaaaaa
    25561 gtgcgtgaag tttgccacgt cccgctgatt gcctccggtg gcgcgggcac catggaacac
    25621 ttcctcgaag ccttccgcga tgccgacgtt gacggcgcgc tggcggcttc cgtattccac
    25681 aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg caacacaggg cgtggagatc
    25741 aggatatgtt aacagaacaa caacgtcgag aactggactg ggaaaaaacc gacggactta
    25801 tgccggtgat tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc tatatgaacc
    25861 cggaagcctt agacaaaacc atcgaaagcg gcaaagtcac cttcttctcg cgcactaaac
    25921 agcgactgtg gaccaaaggc gaaatgtcgg gcaatttcct caacgtagtg agtattgccc
    25981 cggactgcga caacgacacg ctactggtac tggcgaatcc tatcggacca acctgccaca
    26041 aaggcaccag cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg tatcaactgg
    26101 aacaattgct cgccgaacgc aaatctgccg atccggaaac ctcctacacc gccaaactgt
    26161 atgccagcgg caccaaacgc attgcgcaga aagtgggcga agaaggcgtg gaaaccgcac
    26221 tggccgcgac ggtacatgac cgctttgagc tcaccaacga ggcgtctgat ttgatgtatc
    26281 acctgctggt attgttgcag gatcaggatc tggatttaac aacggtgatt gagaacctgc
    26341 gtaaacgtca tcagtaagtt gcggggtaag cggatgcgat gttgtcgtcg catccggcaa
    26401 aaaaaacggg caaggtctca ccaccctgcc cattttcttt aaaaccgaaa agattacttc
    26461 gcgttgtaat tacgcagagc attacgcccc aacacaatcc ccgcgccaac catgccgccc
    26521 agcagcaccg ccagaatcaa ggtgattgcc tttttcggac tatcgcgacg aataggtaac
    26581 gtcggtttca tcacataacg gtaagcatga atatcaagat catcaacttt taagctttcg
    26641 atatccagca ggttttgacg agtctgatag tagtttggcg agaacaccaa cggacgggtc
    26701 gcctcatgct taatcatcga ctccagcgct tcgctaccca aaaggaacaa cgtatcttgc
    26761 gtcacatctt cagtctgctg aacctgtggc tttgtcacct gcgcctgatt cgcatactgc
    26821 aacgcttcct gaatctgacg gatacgcaga tctttctgct cctgcgcaac tacttcctgc
    26881 gttcttaaag agtcctgcaa gtttttccgt cccagagcaa tgttgtcctt gagatccttt
    26941 tctaactctt gattcacttt atcgtcaact tgctgaatgt attgggccaa cttcatttgt
    27001 gcgccctctg cagtttgccc aacataagaa acagttaatg gtaattgctg gttcttaaca
    27061 gaaggttcga tggtaagttt ttctggttct tcctgattat ccagcgtttc tgctaatgca
    27121 gagaaggcag aactgaagcg accaattaac gtctcctgca aatccgatac tttcggtgca
    27181 gcctgaccat agataacatt catggcattg ttatagccag caatttgccc cacgtcgggc
    27241 tgagtgataa ttgctgttga cgtccatttc tccttcgcta ctgccaaata tccaatagcc
    27301 agggcaatag ccacaatgac ggaaatgatg attgtcatct tgccacgcca caactgcact
    27361 agtaaatcaa tcaaatcaat ctgttccggg tcatggtttt gcccagaaac attattattt
    27421 tctactctca tcattaccct aactgacgga aatttccaag tccgcatagt ttagcgataa
    27481 tcctcgtgaa tgctatagga taagtgataa aaataaagaa ttgttgagga agtaacaaaa
    27541 gacaatcagg gcgtaaatag ccctgataac aagatgttag tcgctgccaa agagatcgcg
    27601 ggtgtatacc ttatctgcca catccttaag ctcttctgcc atacgattgg agataatgac
    27661 gtcagcttgt tgtttgaaag tggcgagatc tcgttccagg cgagagttga agaatgagtt
    27721 ttctttcatc accggttcgt aaatgatgac ttcaacgcct ttcgccttga tacgtttcat
    27781 aatcccctga atggaagacg cgcggaagtt gtctgaaccg ctcttcataa tcagacgata
    27841 aatacccacc acttgcggct tgcgtgacaa aatggcatcg gcaataaaat ctttacgcgt
    27901 gcggttagca tcgacaattg ccgaaatcag gttattcggc acagactgat aattcgccag
    27961 taactgtttg gtatctttcg gcaaacaata accaccataa ccaaacgacg gattgttgta
    28021 atggttgccg atacgcgggt cgagacaaac accttcgata atctgacgcg tattcagacc
    28081 caaactttct gcatagctat ccagttcgtt aaagtacgcc acgcgcatcg ccagataggt
    28141 attagcgaaa agtttaatcg cttctgcttc agtggagtcg gtaaacaggg tcgggatatt
    28201 ttgcttaatc gcgccttcct gtaacaacgc agcaaaacgt tctgcgcgtt ctgaacgctc
    28261 accgatgaca atacgtgacg gatgaaggtt atcgtaaagg gctttaccct cacggagaaa
    28321 ttccggggag aatataatat tttcagtacg atatttttta tgcatcgctg cggtaaaacc
    28381 aacgggaacc gttgatttga tgaccataac cgcataaggg tttatctcaa ctacgtcttt
    28441 aattactgat tctacactgg atgtattgaa ataattagtt ttaggatcat agtcggttgg
    28501 agtggcgatg atgacataat cagcaccccg gtaggcttca tttttatcta atgtggcatt
    28561 aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc ttatcgacaa taggagatat
    28621 ccgatcattc agcatagcaa cgcgtgacgg taaaatatct aatgccacaa cctcatgatt
    28681 ttgtgcgatt agaagcccgt ttgataagcc tacatagcca gtaccggaaa tggtgatttt
    28741 catttcatgc tctcagaatt aacttaactg tgaatcatga tgtttttagc atcctgataa
    28801 gagctaaaag ttttaacgct acgttgtcag atggtcaacg caacgaaaaa caattcagat
    28861 tttctttata agaatagctg atatttatta agttaatatt aagcagtgaa atttagtcta
    28921 taagatattt ggcaaaaaaa agcccggtgc aatatacgcc gggcctcaat tttattgttg
    28981 gttaaatcag attaatccag ccattcagta tggaacacac cttctttatc aatgcgctta
    29041 taagtatgcg caccgaaata gtcacgctgt gcctggatca ggtttgcagg cagaactacg
    29101 gcgcggtagc tgtcataata ggcaaccgct gccgcgaagg tcggaaccgg gataccgttc
    29161 tgtactgcat aagcaacgac atcacgcagc gcctgctggt agtcatcggc aatttgcttg
    29221 aagtacggag ccagcagcag gttagcgatc tgcggatttt cggcataagc atcggtgatt
    29281 ttctgcagga actgcgcacg gatgatgcag ccagcacgga aaatcttcgc gatttcgccg
    29341 tagttcagat cccagttgta ctcttcagac gcagcacgca gctgagagaa accctgcgcg
    29401 taagaaacga ttttgcccag atacagcgca cgacgaactt tttcgatgaa ctcagctttg
    29461 tcgcctgctg gctgcgcttg cggaccagag agaactttag acgcggcaac acgctgatct
    29521 ttcagagaag agatataacg tgcaaaaaca gactcggtaa tcagcgacag cggttcgcca
    29581 agatccagcg cactctggct ggtccatttg cccgtacctt tgtttgctgc ttcatccagg
    29641 atcacatcaa ccaggtagtt accgtcttca tcttttttgg tgaagatatc tttggtgatg
    29701 tcgatcaggt agctgctcag ttcaccgtta ttccactcgg taaaggtctg cgccagttct
    29761 tcgttggaaa gattcaggcc acctttaagc agagaatagg cttcagcaat cagctgcata
    29821 tcgccgtatt caataccgtt gtgaaccatc ttcacatagt gacctgcacc atcggcacca
    29881 atataggtaa cgcatggctc cccgtcttca gctactgcgg cgattttggt caggattggt
    29941 gcaaccagtt cataggcttc tttctgccca ccaggcataa tagaaggacc tttcagcgcg
    30001 ccttcttcac caccggagac accggtacca atgaagttaa agccttcgtc agaaagttca
    30061 cggttacgac gaatggtgtc ctggaagaag gtattgccac catcaatgat gatgtcacct
    30121 ttatcgaggt aaggcttgag ggaatcaata gcagcatccg tgcctgcacc tgctttcacc
    30181 attaacagga tgcgacgagg cgtttccaga gattcaacaa actctttcac cgtatagtaa
    30241 ggaaccagtt ttttgcctgg attttcggca atcacttctt ccgtcttctc acgggaacgg
    30301 ttgaaaatag agacggtata accacggctt tcgatgttga gcgcaaggtt gcgccccatc
    30361 actgccatac cgactacgcc gatctgttgc tttgacattg tttactccag tcagggggat
    30421 catgaagagg tgtagtttaa gaattaaatt tcgattcaac tatgagttta taggtagtaa
    30481 attttaagtt aaatagatta gttttataat cttataaata gctgcgccta ataccgctac
    30541 acttttgcca gcccatgttt gccttagggc atggctgacg gtttccataa catggcgaac
    30601 ttttttcagc agttgccata ttgaccggca ctgatgattg cgcgttattg tgtcgcgaag
    30661 tgcctgccat agccgttcca catgatgcac caatggcgag taaaccggct gataaataac
    30721 tctgaacttc ggttctcctt cagccagcgc tgtgtttccc ggcttttatg gataatgtag
    30781 ttgtccacga tcagcgtgat ggttttcgcc cgacggtatg tcgctttaag ccgcttcagc
    30841 aggctgatga acagcgttga acttttgctg ttgccggcca gataatattt ttccttctgc
    30901 cccggtgtta ccacgcgttt ctgttgtcca cgtagctgcc agtctgcacc gattttggga
    30961 ttaagatgga tatacacttc atcttcataa aagaccggat gctctgcgca gcattcgtcc
    31021 agtgctttgt ggattgccgc catcttttca tctttatgcg ggtcaaggat acgcagggtt
    31081 ggtgcggccc ttcgccacac aagccctgca gacggcaacc agcggcaaac ggtcccggca
    31141 tataactggc agccggttat ctcattgatt tttattgcca gaagttctgt actcaagcct
    31201 gagcgctggt agccaaaatc gccgtgagca tgtttaccag ctcacgtaat aaagtgcaga
    31261 tatgctcaaa aggccagcgt ggggtcgtcc ggcagataat gatttcagtc ttgcaacacc
    31321 cgacagcgtg aaccagttaa tccagcgtcc gacgaaggaa ctgccacggc agagcattct
    31381 gccaacatcg ctaacgaggt cgcccgatgc agcatcagta tggccatcag tctgcgggca
    31441 taatttttat cgtgcgtttt ctggattgct ttctgcatca ggcgtcgttc gtcacgggga
    31501 attggtgcta tgatcggcat cgctcagtcc ggttggtgat ttgttttgat ttgatgattg
    31561 atcagatcaa acaatccggg ctgagttccc ttaaagtgat ctactattcc gcgcagctat
    31621 ttaaggtata ttagaaccta ccaattaaaa taagaaactt aactagaata ctttcgtatt
    31681 tcttagcata tttaacccct aagcccataa ccaacttaat tctaagaaaa atat
//
LOCUS       DAZFFG010000044        31663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFFG010000044 DAZFFG010000000
VERSION     DAZFFG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.044"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1407
                     /locus_tag="ACHJ7J_002378"
     CDS             <1..1407
                     /locus_tag="ACHJ7J_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5000602.1"
                     /translation="CGYTEKNTTRNVLLLFFLLLLAHRNTLTSITALLFLFCCALYAP
                     AGMTYGKINNSFIVALLQTTADEAAEFTGMIPVYHFLVSAAILVFMVIFWRTHHRGHR
                     NWLALLLFVLCSVNSWPLRMVKGTVVGTTDTLREMQRYKQLSQHGADSWKILPGVPLY
                     DTIVIVTGESVRRDYMSVYGYPVPTTPWLNTAPGLFIDGYTSAAASTVPSLSRTLIYD
                     YEQNPDSGNNVVALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHTVFLKKGSFAS
                     RKTDDMQLLQETERALADTSSPKVIFLHMMGSHPNPCDRLNSWPNNYQEQFPRKIACY
                     LASISKLDNFLGQLDGILRRHSRHFAMLYFSDHGLSVSDSTNPVHHDGHVQGGYSVPL
                     IITASDITSHQSVSRKISARHFAGIFQWLTGIRTENIPPFNPLTDEDNEPVMVFNGEK
                     NVPADSLKPQPLILPDHR"
     gene            complement(1712..2977)
                     /locus_tag="ACHJ7J_002379"
     CDS             complement(1712..2977)
                     /locus_tag="ACHJ7J_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5000603.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TRDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(3175..3250)
                     /locus_tag="ACHJ7J_002380"
     tRNA            complement(3175..3250)
                     /locus_tag="ACHJ7J_002380"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3215..3217),aa:Phe,seq:gaa)
     gene            complement(3356..4063)
                     /gene="yqgA"
                     /locus_tag="ACHJ7J_002381"
     CDS             complement(3356..4063)
                     /gene="yqgA"
                     /locus_tag="ACHJ7J_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO5000604.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            4216..4299
                     /gene="yqgH"
                     /locus_tag="ACHJ7J_002382"
     CDS             4216..4299
                     /gene="yqgH"
                     /locus_tag="ACHJ7J_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO5000605.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            4461..6596
                     /gene="speC"
                     /locus_tag="ACHJ7J_002383"
     CDS             4461..6596
                     /gene="speC"
                     /locus_tag="ACHJ7J_002383"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO5000606.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESQEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(6646..7902)
                     /gene="nupG"
                     /locus_tag="ACHJ7J_002384"
     CDS             complement(6646..7902)
                     /gene="nupG"
                     /locus_tag="ACHJ7J_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO5000607.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(8104..9183)
                     /gene="mltC"
                     /locus_tag="ACHJ7J_002385"
     CDS             complement(8104..9183)
                     /gene="mltC"
                     /locus_tag="ACHJ7J_002385"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO5000608.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(9248..9523)
                     /gene="yggX"
                     /locus_tag="ACHJ7J_002386"
     CDS             complement(9248..9523)
                     /gene="yggX"
                     /locus_tag="ACHJ7J_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO5000609.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(9551..10603)
                     /gene="mutY"
                     /locus_tag="ACHJ7J_002387"
     CDS             complement(9551..10603)
                     /gene="mutY"
                     /locus_tag="ACHJ7J_002387"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO5000610.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            10764..11483
                     /gene="trmB"
                     /locus_tag="ACHJ7J_002388"
     CDS             10764..11483
                     /gene="trmB"
                     /locus_tag="ACHJ7J_002388"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO5000611.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            11483..11809
                     /gene="yggL"
                     /locus_tag="ACHJ7J_002389"
     CDS             11483..11809
                     /gene="yggL"
                     /locus_tag="ACHJ7J_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO5000612.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEERIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            11993..12712
                     /gene="yggN"
                     /locus_tag="ACHJ7J_002390"
     CDS             11993..12712
                     /gene="yggN"
                     /locus_tag="ACHJ7J_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO5000613.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            12888..13934
                     /gene="ansB"
                     /locus_tag="ACHJ7J_002391"
     CDS             12888..13934
                     /gene="ansB"
                     /locus_tag="ACHJ7J_002391"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO5000614.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            14051..15058
                     /gene="yggM"
                     /locus_tag="ACHJ7J_002392"
     CDS             14051..15058
                     /gene="yggM"
                     /locus_tag="ACHJ7J_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO5000615.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYMIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINKACDRWKKDTEQLRRNGVI
                     K"
     gene            complement(15214..16350)
                     /gene="hemW"
                     /locus_tag="ACHJ7J_002393"
     CDS             complement(15214..16350)
                     /gene="hemW"
                     /locus_tag="ACHJ7J_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO5000616.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(16343..16936)
                     /gene="rdgB"
                     /locus_tag="ACHJ7J_002394"
     CDS             complement(16343..16936)
                     /gene="rdgB"
                     /locus_tag="ACHJ7J_002394"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO5000617.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREQKSAISHRGQALKLLLDALRNG"
     gene            complement(16944..17234)
                     /gene="yggU"
                     /locus_tag="ACHJ7J_002395"
     CDS             complement(16944..17234)
                     /gene="yggU"
                     /locus_tag="ACHJ7J_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO5000618.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(17231..17797)
                     /gene="yggT"
                     /locus_tag="ACHJ7J_002396"
     CDS             complement(17231..17797)
                     /gene="yggT"
                     /locus_tag="ACHJ7J_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO5000619.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(17815..18519)
                     /gene="yggS"
                     /locus_tag="ACHJ7J_002397"
     CDS             complement(17815..18519)
                     /gene="yggS"
                     /locus_tag="ACHJ7J_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO5000620.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            18537..19517
                     /gene="yggR"
                     /locus_tag="ACHJ7J_002398"
     CDS             18537..19517
                     /gene="yggR"
                     /locus_tag="ACHJ7J_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO5000621.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLNCMTFALGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(19708..20124)
                     /gene="ruvX"
                     /locus_tag="ACHJ7J_002399"
     CDS             complement(19708..20124)
                     /gene="ruvX"
                     /locus_tag="ACHJ7J_002399"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO5000622.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(20124..20687)
                     /gene="yqgE"
                     /locus_tag="ACHJ7J_002400"
     CDS             complement(20124..20687)
                     /gene="yqgE"
                     /locus_tag="ACHJ7J_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO5000623.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(20796..21746)
                     /gene="gshB"
                     /locus_tag="ACHJ7J_002401"
     CDS             complement(20796..21746)
                     /gene="gshB"
                     /locus_tag="ACHJ7J_002401"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO5000624.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(21759..22490)
                     /gene="rsmE"
                     /locus_tag="ACHJ7J_002402"
     CDS             complement(21759..22490)
                     /gene="rsmE"
                     /locus_tag="ACHJ7J_002402"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO5000625.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(22570..23277)
                     /gene="endA"
                     /locus_tag="ACHJ7J_002403"
     CDS             complement(22570..23277)
                     /gene="endA"
                     /locus_tag="ACHJ7J_002403"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO5000626.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(23372..23869)
                     /gene="yggI"
                     /locus_tag="ACHJ7J_002404"
     CDS             complement(23372..23869)
                     /gene="yggI"
                     /locus_tag="ACHJ7J_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO5000627.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(23946..25340)
                     /gene="galP"
                     /locus_tag="ACHJ7J_002405"
     CDS             complement(23946..25340)
                     /gene="galP"
                     /locus_tag="ACHJ7J_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO5000628.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(25777..26931)
                     /gene="metK"
                     /locus_tag="ACHJ7J_002406"
     CDS             complement(25777..26931)
                     /gene="metK"
                     /locus_tag="ACHJ7J_002406"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO5000629.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            26987..27238
                     /gene="yqgD"
                     /locus_tag="ACHJ7J_002407"
     CDS             26987..27238
                     /gene="yqgD"
                     /locus_tag="ACHJ7J_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO5000630.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            27349..27492
                     /gene="yqgG"
                     /locus_tag="ACHJ7J_002408"
     CDS             27349..27492
                     /gene="yqgG"
                     /locus_tag="ACHJ7J_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO5000631.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            27586..27717
                     /gene="yqgB"
                     /locus_tag="ACHJ7J_002409"
     CDS             27586..27717
                     /gene="yqgB"
                     /locus_tag="ACHJ7J_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO5000632.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            27726..29702
                     /gene="speA"
                     /locus_tag="ACHJ7J_002410"
     CDS             27726..29702
                     /gene="speA"
                     /locus_tag="ACHJ7J_002410"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO5000633.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            29848..30579
                     /locus_tag="ACHJ7J_002411"
     CDS             29848..30579
                     /locus_tag="ACHJ7J_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000634.1"
                     /translation="MKKWKVRSALIALVVLLVGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRA
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            30715..31635
                     /gene="speB"
                     /locus_tag="ACHJ7J_002412"
     CDS             30715..31635
                     /gene="speB"
                     /locus_tag="ACHJ7J_002412"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO5000635.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         7732 a         7900 c         8299 g         7732 t
ORIGIN      
        1 tgcggataca ctgagaagaa taccaccagg aatgtgttgc tgctgttctt tcttctgctt
       61 cttgcacaca gaaataccct cacatccatt acagcgctgt tatttctgtt ctgttgtgca
      121 ctgtatgcgc ctgccggtat gacgtacggt aaaatcaaca acagttttat tgtcgcgttg
      181 ttgcagacca cggctgatga ggcagcggag tttaccggga tgattcctgt ttatcatttt
      241 ctggtcagtg ccgcgattct ggtattcatg gtgattttct ggcgaacaca ccaccgcggt
      301 caccgtaact ggctggcact gctgctattc gtattatgct ctgtaaacag ctggccgttg
      361 cggatggtta aaggaaccgt tgtggggaca actgacacat tgcgtgaaat gcagcgttat
      421 aaacaactga gtcagcacgg ggctgacagc tggaaaatcc tgccgggtgt accgttgtat
      481 gacacgattg ttattgttac tggtgagagt gtgcgcaggg attatatgtc ggtatatggc
      541 tatcccgtgc caaccacacc gtggctgaat actgctcccg gtttatttat tgacggctat
      601 acatcggcag cagccagtac cgtaccttcc ctgagccgga cactgattta tgactatgag
      661 cagaaccctg actccggcaa caacgtagtg gcgctggcag caaaagcagg atacagcaca
      721 tggtggatat ccaatcaggg aaaactgggg gagcatgaca cacgcatctc tgttattgct
      781 tctgatgcgg agcataccgt tttcctcaag aaaggcagct ttgcttcccg taaaacggat
      841 gacatgcagt tgttacagga aacagaacgt gcgctggcgg atacatcctc tccgaaggtc
      901 attttcctgc acatgatggg ttctcatccg aatccgtgtg accggcttaa ctcctggccg
      961 aataattacc aggagcagtt cccacggaag attgcctgtt acctcgccag catcagtaaa
     1021 ctggataact ttctcgggca gcttgatggt atccttcgcc gacattcccg tcattttgcc
     1081 atgctttact tttctgacca tggactgtca gtcagcgaca gtactaatcc tgttcatcat
     1141 gatggccatg tgcagggtgg ctacagtgtt cccctgatta ttaccgccag tgacatcacg
     1201 tctcaccagt ccgtcagcag aaaaatcagt gcccgtcatt tcgcaggtat ttttcagtgg
     1261 ctgaccggca ttcgtaccga aaatataccg ccattcaatc cgctgacaga cgaagataat
     1321 gaacccgtta tggtttttaa cggagagaaa aatgtgccgg cagacagtct gaaaccgcag
     1381 ccacttattc ttccggacca caggtaatca tatatggcaa actgtaatac agtctcttgt
     1441 actgaaaaat agaatctgta ttcatatcag cacaagggac caccaaatcc cggcagcatt
     1501 ccttacgtca gttatcccgc atcaccctgt gagtgagttt ttttatgagg ttactttttc
     1561 attgaatata aataatgaat aaccgcagat tctgataatc ctcctccagg gaacaatcag
     1621 gtaaacagta aatacatcag ttaataccat tttgtctttt taacaaagaa aatattcatg
     1681 gagtaagttc ccgggcattt gcccgggatg attactgttt taatggatta ttaatctttg
     1741 catattcaaa tggactgata aatctttctc tgtttgcatc cagaaaatcg gcccaccatt
     1801 gtagcatcaa gcggcgttct tccagatgtt ctgctttatg aatgtaagca gcacgtacac
     1861 cgttacgttc ctgatggctc atctgacgtt caacagcatc gcgggaccac aaacctgatt
     1921 ctattaacgc actacatgcc atcgcccgga acccatgacc acagacatcc cgggttgtgt
     1981 cataccccat aaccctcagg gctttattta cggtgttttc actgattggt ttacgtgggt
     2041 catgtgctcc cgtaaagatc agaccatttt caccagccca tgtctgaagt tctgccagta
     2101 tggctacagc ctgtttgctg agtggcacaa gatgtggtgt tcgcatcttg gatccccggt
     2161 gtgagaattt tacgcctggc agaggttcgc gctcagctgg aatagtccac atggcattgt
     2221 caatatcaat ttccgaccag cgggcatatc tgagttcact ggaacgaata aaaaccagta
     2281 aattcagcat aacggcaagc tgggtgacag gctgcccctt gtaactgcca atacgcgaca
     2341 aaagttcagg caggcgagaa agatcaagag cggggcgatg ctggcgtttt accgtagtca
     2401 atgcgccagc catatccaga gcaggattat atctgatgat gccagactgt acggcataac
     2461 gcattacggc agatattctt tgctgcagac gactggcgat ttcataaatt tgtttagctt
     2521 ctgcagcacg aacagggata agcaggtccg gggtattgag tgttgtgata tcccgattgc
     2581 cgataaaggg gaatacgtgt gtctcgaggc ttttgagtac tttatcgcta tgtgattgtg
     2641 cccattgttt attactggca tgccagcgtc gtgcaatatg ttcgaaagag agttgtaaat
     2701 cagggactac ttttttagct cttttctgtt cgcaagggtc aatcccctcc gaaatcagtt
     2761 ttcgggcctc atcccgtttt gtacgagctt cggagagaga aacttctgga tacacaccaa
     2821 gtgccagagt tttctcctta cccagaatac gataacggag acgccagtaa cgggaaccat
     2881 tgggatgtac cagcaggaac atgccagccc cgtcagtgag tttataagcc ttgtcagtgg
     2941 gctttgcagc ccggattttt gcgtcagtca gtgccatagt tcagccttct gaatgcgtta
     3001 aagagggtac aaattctgtc gaactggggt atacccgcaa ttatacccgc acaatggggt
     3061 tgatgtgggg ggaatctggt tgagttcggt cgaattgatt tggaggaaga acacttaaat
     3121 catggtgttc tgaaacgaaa aacggacctc cgtggaggtc cgttgatatg aatttggtgc
     3181 ccggactcgg aatcgaacca aggacacggg gattttcaat cccctgctct accgactgag
     3241 ctatccgggc aacggggcgc attaaacctg attcgcctcg tctcgtcaat caaatttctt
     3301 caatttactg cagactgcac aatctatcat cactttgccg ctattgcacg cattgtcagg
     3361 caaaccaggc ggtccaggcg gcggaaagcg gcattgccag aaggagtgct ggaagcatgt
     3421 tgaccaccgg gaacatttta atgccacaga tgcgtaatcc ggttgccagc agcaataaac
     3481 cgcctacggc gctgaagtct gccatcatcg acggtgtggt cagcggtaat atcagcgcgg
     3541 cagcccacgc cagcgttaac tggatgatca gtaatgggat actaatcacc gataccgcaa
     3601 tacccagcga gcaggcgaag atcatcgccg taaagaaatc aagaaatgac ttggcgatta
     3661 aaatactcgg atcgccggtc atcccttcgt tcatcgcccc gaagatcccg gtgccgctgg
     3721 cgcaaaacag gacaataatc gcgacataat tctgaataaa agattcatgc gctggcttct
     3781 tacgtgagtg acgaaacaga ttttgtgctt tggcgaccgc tgtattgacg cctttctcca
     3841 gcagacaaat ttcgccgatt aatgctccga gtaaggttgc taaaaccatc gccggaaggt
     3901 tggcgcattt caccaccagt aaaataccaa tccccagcga tgccaaacca aaaattgatg
     3961 tcatggagac gcggatacgt tccggtaagc gttggctaag cagtgcaccg agcatgccac
     4021 ccagtaatat tgcacttgcg ttgataaaag ggccgatgac cacaagagtt cctgttaact
     4081 gtattgtttg attttcttat tatgaacttt ttgccttgtt gatggcttgt ctggtcgcaa
     4141 agtcctggct tgcacgcctt agcgaaaggt gccatgattg cgcgaatttt ctcctctctg
     4201 tacggagttt gcccgatgca cgccatctcc ttacattctc tcgcttatcg ccgtttcgcg
     4261 cgaaacgttt ccctttctat gttactgctc atgcggtgaa gttaacgcac gctcactgca
     4321 ggacaacagt aaaatcagag cgtttctgct tttactgatg tctggcggtc ggagctggtg
     4381 accagtttga cccatatctc atggggcagg gttttccacc ttgtccggta ttcttacttc
     4441 cccgaaacgg gtttgcgctt atgaaatcaa tgaatattgc cgccagtagt gaactggtat
     4501 cccgactttc tactcatcgc cgcgtggtgg cgttgggaga tactgatttt acggacgtcg
     4561 cggcagtcgt cattaccgct gcggatagtc gcagtggcat tcttacgttg cttaagcgca
     4621 ccggttttca tctaccggtg tttttgtatt ccgaacatgc tgttgaatta cctgcgggcg
     4681 ttacggcggt aatcaacggc aacgagcagc attggctgga gctggaatcc gcagcctgtc
     4741 agtatgaaga gaatttgctg ccaccgtttt atgacacgct gacgcagtac gttgagatgg
     4801 gcaacagcac ctttgcttgc cctgggcatc aacatggtgc gttttttaaa aagcatcctg
     4861 ccggacgcca tttttacgat ttctttggtg agaacgtctt tcgcgccgat atgtgtaacg
     4921 ctgacgtaaa attgggcgat ctgcttattc atgaaggatc ggcgaaagat gcgcagaaat
     4981 tcgcagccaa agtctttcat gccgataaaa cctattttgt gctgaacggc acatcggcag
     5041 cgaataaagt ggtgacgaat gcgctgttaa cgcgtggcga tctggtgctc ttcgaccgta
     5101 acaaccataa gtcgaatcat catggcgcgt tgattcaggc gggggcgacg ccggtctatc
     5161 tggaagcttc acgtaacccg tttggtttca ttggcggtat tgatgcgcac tgttttaatg
     5221 aagagtatct gcgccagcaa attcgcgacg ttgcgccaga aaaagccgac ctgccgcgcc
     5281 cgtttcgcct ggcgattatt cagctgggaa cctatgacgg cactgtctat aacgcccgtc
     5341 aggtgatcga taccattggg catctgtgtg attacattct gtttgattcc gcgtgggtcg
     5401 gttacgaaca gtttatcccg atgatggcgg atagctcgcc gctgctgtta gaacttaacg
     5461 aaaacgatcc agggatcttt gtgactcagt cggtgcacaa acagcaggcg ggattctcac
     5521 agacgtcgca gatccataaa aaagataacc atatccgcgg acaggcgcgt ttttgcccgc
     5581 ataagcggtt gaataacgcc tttatgctcc atgcttctac cagccctttc tatccgctgt
     5641 ttgctgcact ggatgttaac gccaaaattc atgaagggga gagtgggcgt cggctgtggg
     5701 ctgagtgcgt tgagttgggt attgagtcgc gcaaggctat tcttgcgcgc tgtaagctgt
     5761 tccgcccgtt tatcccgccc gttgttgatg gcaaattgtg gcaggattat ccgacatcag
     5821 tgttagccag cgaccgccgt tttttcagtt ttgagccggg ggcgaagtgg cacggctttg
     5881 aaggatatgc cgcggatcag tattttgttg atccgtgcaa gctgttactc actacaccag
     5941 gtatcgatgc cgaaaccggc gagtatagcg actttggcgt tccggcgacg attctggcgc
     6001 actatctgcg tgagaacggc attgtgccgg agaagtgcga tctcaactcc attctgttct
     6061 tattaactcc ggcggaaagc caagagaagc tggcacaact ggtggcgatg ctggcgcaat
     6121 ttgaacagca tattgaggat gactcgccgc tggctgaggt gttgccgagc gtttataaca
     6181 aatatccggt gcgctatcgc gactacaccc tgcgccagtt gtgtcaggag atgcacgatc
     6241 tgtatgtcag tttcgacgtc aaagacctac aaaaagcgat gttccgccag cagagtttcc
     6301 cgtcagtggt gatgaacccc caggatgcgc atagcgctta tattcgcggt gacgtggagt
     6361 tggtgcggat tcgtgatgcc gaagggcgaa ttgcggcaga aggggcgttg ccttatccac
     6421 ctggcgtgct ttgcgtggta cccggggaag tctggggtgg ggcggttcaa cgttatttcc
     6481 ttgcgctgga agaaggggtg aatttgttgc cgggattttc gccggagctg caaggtgttt
     6541 atagcgaaac cgatgcggat ggcatgaaac ggttgtacgg ttatgtgttg aagtaagaat
     6601 aaaaaaaacg ggtcaccttc tggcgacccg tttttctttg cgtaattagt ggctaaccgt
     6661 ctgtgtacct gtcgggacac gaacgtgttt atatttgaac atcgccatga acgcgaaggc
     6721 cagaaccacg gagtaaccag cgaaaatcaa ccatacggtc tgccagtcgg taatgccgtt
     6781 ttgggtgtac atctcaacaa ctttaccgct cacgatgccg ccgaggatac agccgaagcc
     6841 gttggtcatc atcaggaaca tcccttgcgc actggcgcga atcgccgggc taacttcttt
     6901 ttcgacaaac accgaaccag agatgttgaa gaagtcgaac gcacagccgt aaacaatcat
     6961 cgacagaacc agcagtacgg tgccgaacgg agtcgggtcg ccgtaagcaa acagcgcaaa
     7021 acgcaggatc cacgccacaa tactgatcat cattacgttc ttaataccgt agcggcttaa
     7081 gaagaatggg atggtcagaa tgaacagggt ttcagagatc tgcgaaatcg acatgatgat
     7141 tgacgcatgc tgcacaataa agctgctggc aaacatcgga tctttgtcga agctgtgcag
     7201 gaaggtatta ccgaacatgt tggtaatctg cagttccgcg cccagcagca ttgagaagat
     7261 gaagaagatt gccatacgct tgtttttaaa cagcgcgaat gcatcgaggc ccagcagggt
     7321 tgtccagctc tgattcgctt gctgtttagc aaccggaata tgcggcagag tcagggtaaa
     7381 cagaaccaga atggcggaaa gtgctgcgcc aatatacagc tgcatgtggc ttaattcgaa
     7441 gccagacagg ctcaccaccc acattgccat gataaagccg atggtgcccc agatacggat
     7501 tggcgggaag tcagtaacga tatccatccc ggcattttgc aggcgatagt aagagatggt
     7561 gttgattaac ccaagcgttg gcatataagc aaacgagtta atcaatatca caaggaacat
     7621 cgcctccggc gtcgtgacct gtgccgccat gaacagcgtg atagcgccaa tggtgtggca
     7681 aatggcatat acccatttcg cacttaacca tttgtcggcc acaatcccca gcagcgcagg
     7741 cataaagacc gcggcgatac ccagtgagct ataaactgcg ccaatagaag caccgtcaaa
     7801 cttcagggta acaaacatat aggaaccgag ggtcgtcagc caacttcccc acagacagaa
     7861 ctgcagaaaa gagaggattt tcagctgcag cttaagattc atgttaattt cctcacatcg
     7921 tgatgcggat gagtgtttga aaaagcacat ttgaccgtgc gagatgatgg gattctatca
     7981 acttcgcgga ctggtttttt gttacctgtg gcaaataatt gcaaacattc ctaatttgca
     8041 aaacgaaatt gtgatatgga taacattttt gcccctgagc atcgtcaggg gcggttagtg
     8101 gaattatcgg cggcggtagg atttttgcgc ggtattcact ttataaagat aacggcgaga
     8161 ttccgcagag ggatggcggg tcgtcagcgt ttgataaaca tcgcccggcg tcatggtgtt
     8221 aataatatta gccgcctgaa tcttatcatt cgaaaagact cgcagcacgc tgcctgcgcc
     8281 gccgttatag gcggtgatga cggcataacg ccgggatgta gggttatcaa ttccgccgag
     8341 ataaacattg ttcagcatcg ccagatacgc ggtgccggta tcaatattgc tggcaggatc
     8401 aaacaagaaa ctgcggctcg gcgtgccgga tttcccctgc gagcggaaca catctttccc
     8461 ggcagtatgt tgtaccacct gcattaaccc cagcgcatcg gaacggctga ccgcatacgg
     8521 gttaaaggaa gattcagtct gcataattgc cagaatcagc gactcatcaa cgccatattt
     8581 ccgtgacgcc tggcggacca tgccgagata tttgtgcgca cgtttatcaa ggtggttcgg
     8641 caccatgtta atggtgacgc tgtagatgat acgcagcccg ttgctgcggc tcttcagacg
     8701 gtttttcagc agataatccg cgaagttgct tgcgcgacct tcccagcgaa tcggctgccc
     8761 ggtgttgtcc accacctgac cgtaaaggaa aggttctttc gaaattgtaa tatcatcaac
     8821 gtcggaatag agatcgaccg aactcggatc gtcacccatc agcaacgttt tgataattgc
     8881 ccggcgcaaa tgcgcggcag gttctgtccc ggcgatggtt tcgatagtaa tcgtaccgtc
     8941 atcgaagttg atgtggctgc gggtctgata ttgatcggtg tatttcacgt agtccttagg
     9001 accggcgatc accacctctt tgaagcccca gatgttctca atattgtggg caaattgccc
     9061 catcagaata tcaaaaccgt tggtgtcttt gacccaggct tcgttatagg tatcgccttt
     9121 tttggtcgtc gaacaggaga tgagcaacgg cgcaatcaaa gccagcgcga gatatttttt
     9181 catcattccg ggtgcgtgtt gtgtttgttt ttatgcaagc tgccggaggc gtgctccggc
     9241 actgttttta ttttttatct tccggcgtat agccctcgat atgcacctct ttaccctcga
     9301 acaggaagtt gaccatctcc tgctccagca gcttgcggtg ctcggcattc atcatgttga
     9361 gtttcttttc attaatcagc atggtttgtt tgtgctgcca ctgcgcccag gcttctttgg
     9421 agatctcgtt atagatgcgt tttcccagtt cgccggggta cagctgaaaa tcctgacctt
     9481 ctgcttcacg ttgcaggaaa gtacaaaaaa tcgttctgct cataaatcat cctctttatc
     9541 gactcacgcg ctaaaccggc gcgccagtgc gtaactgctg taacaaacgc tccacgggag
     9601 ccgccaggcc aactgacggc ggttgcgcta agttatacca gagcgcattg ccttcatcca
     9661 tgcagccggt gaatgacgac acgggaagcc acataggcac aatatctaag tggaaatggc
     9721 tgaaggtatg ccgaaacgcg gtcagttgcg tcaggttatc ggcagcaatc tgccgttgcg
     9781 ccagccactg ccgcaaactt tcttcgtcgg caaactgcgg gaaacagtat aaaccgcccc
     9841 acaatccgct cggcggacgc tgcgccagca atacttcatc ttcgtgctgt aacagcaaaa
     9901 agtagccggt gcgctccggc agcgtctgtt tcggtttttt gcccggataa agcgaccagc
     9961 tattgttggt ggcggcaata catccgtttt gtagcggaca gagcgaacat ttcggcttcg
    10021 aacgcgtgca aatcatcgcg cccaaatcca tcatcgcctg attaaaccgt tccacgccga
    10081 ccgcgggcgt cacctgctcg cttaaactcc ataatttatt ctcaacctct tttttcccag
    10141 gccagccgct tacagcatag cagcgcgcca gcacccgttt gacgttaccg tcgagaatcg
    10201 gaaagtgctt acccagagaa agcgagagaa tcgcgcctgc ggtggaacgc ccgacgcccg
    10261 gcagtgctgc aacttcctca aaggtttccg ggaatttacc gccgtgtaag gtcgccactt
    10321 gttgtgccgc tttatgcaga ttgcgcgcgc gggcgtaata gccaagcccg gtccacaagt
    10381 ggagaacttc gtcgagcggc gcattggcga gatcggtcac cgtcgggaag cgcgccataa
    10441 agcgttcaaa ataggggata acggtcgcaa cctgagtttg ttgcaacatc acttctgaga
    10501 gccatacttt gtagggcgtc ttgtcaattt gccagggcag agtttttcgc ccgtatttat
    10561 cgtaccagtc cagaacctgg gctgaaaatt gcgacgcttg catggtcacc gaattcactg
    10621 ttgttggggg ccagattgca gcacagcgac ggcagggtgt aaaccggaac tttccgcagc
    10681 cacggcctta tcatgcttgc atccggcaac taactttgga taatgcccgt tttcagaaca
    10741 ctttcacaag cgactaaatc tttatgaaaa acgacgtcat ttcaccggaa tttgatgaaa
    10801 acggccgccc actgcgccgt atccgtagtt ttgtgcgccg ccaggggcga ctgaccaaag
    10861 gccaggaaca tgcgctggaa aactactggc cggtgatggg cgttgagttc agcgaagata
    10921 tgctggattt ccccgcgctt tttggccgtg aagcgccggt gacgcttgag attggttttg
    10981 gcatgggggc gtcgctggtg gcaatggcta aagatcgccc cgagcaggac ttcctcggca
    11041 ttgaagtgca ttcaccgggc gttggtgcgt gcctggcttc tgcgcatgaa gagggcttaa
    11101 gcaacctgcg cgtgatgtgt cacgatgcgg ttgaagtgct gcataaaatg attcctgaca
    11161 attcattgcg catggtgcag ctctttttcc ctgacccgtg gcacaaagcg cgccataata
    11221 aacgccgtat cgttcaggtg ccgtttgccg aactggtaaa aagcaaactg cagctggggg
    11281 gcgtattcca tatggcgacc gactgggaac cttatgcgga acatatgctt gaagtgatgt
    11341 cttctattga cggttataaa aacctgtcag agagcaatga ttacgtaccg cgtccggcat
    11401 cacgtccggt gacgaaattt gaacaacgtg gtcatcgtct tggtcacgga gtatgggact
    11461 taatgttcga gagggtgaaa taatggcaaa gaaccgtagc cgtcgtctgc gtaaaaaaat
    11521 gcacatcgac gaattccagg aattaggatt ttcggtggca tggcgattcc cggaaggtac
    11581 atcggaagaa cggattgata aaaccgttga tgattttatt aacgaggtta tcgaaccgaa
    11641 caaactggcc tttgacggca gcggttatct ggcctgggaa ggtctgatct gcatgcagga
    11701 aatcggcaaa tgcaccgaag aacatcaggc gattgtgcgt aagtggctgg aagagcgcaa
    11761 actggatgag gtacgcacca gcgaactttt cgacgtttgg tgggactaag aaagcatacg
    11821 ggcgatgaca aatgcaaaac tgcctgatgc gctacgctta tcatgcctac aaagatgcac
    11881 gatcgagtag gcgggataag gcgtttacgc cgcatccggc atggaaaacg cgtactttgt
    11941 tatcaatctg gggccagcaa atgctggcct gatttgttct tgagggaaga ctatgatgcg
    12001 caaaatgctg ctggcggcag cactttcagt gacggcaatg accgctcacg ccgactacca
    12061 gtgcagcgtc acgccgcgtg acgatgtgat tgtcagcccg caaaccgtgc aggtgaaggg
    12121 cgaaaacggc aatctggtga tcacgccaga cggcaacgtg atgtataacg gtaagcaata
    12181 ttccctgaat gccgcccagc gcgagcaggc gaaggattat caggctgaac tacgtagcac
    12241 gctgccgtgg attgatgaag gcgcgaaaag ccgcgtcgag aaagcccgta ttgctctgga
    12301 taaaattatc gttcaggaga tgggcgaaag cagcaaaatg cgcagccgtc tgaccaaact
    12361 tgatgcgcag ctgaaagagc agatgaaccg cattatcgaa acgcgcagcg atggcctgac
    12421 gttccactat aaagccattg atcaagttcg cgctgaaggc cagcaattag tgaatcaggc
    12481 aatgggcgga attttacagg acagcattaa tgaaatgggc gcgaaagcgg tgctgaaaag
    12541 cggcggcaac ccattacaga atgtgctggg aagtctgggc ggtctgcaat cctcaatcca
    12601 aaccgagtgg aaaaagcagg aaaaagattt ccagcagttt ggcaaagatg tttgtagccg
    12661 cgttgtgact ctggaagata gccgcaaagc cctggtcggg aatttaaaat aatcctctat
    12721 tttaagacgg cataatactt ttttatgccg tttaattctt cgttttgtta cctgcctcta
    12781 actttgtaga tctccaaaat atattcacgt tgtaaattgt ttaacgtcaa atttcccata
    12841 caaagctaag ggataatgcg tagcgttcac gtaactggag gaatgaaatg gagtttttca
    12901 aaaagacggc acttgccgca ctggttatgg gttttagtgg tgcagcattg gcattaccca
    12961 atatcaccat tttagcaacc ggcgggacca ttgccggtgg tggtgactcc gcaaccaaat
    13021 ctaactacac agcgggtaaa gttggcgtag aaaatctggt taatgcggtg ccgcaactga
    13081 aggacattgc gaacgttaaa ggcgagcagg tagtgaatat cggctcccag gacatgaacg
    13141 ataatgtctg gctgacactg gcgaaaaaaa ttaacaccga ctgcgataag accgacggct
    13201 tcgtcattac ccacggtacc gacacgatgg aagaaactgc ttacttcctc gacctgacgg
    13261 tgaaatgcga caaaccggtg gtgatggtcg gcgcaatgcg tccgtccacg tctatgagcg
    13321 cagacggtcc attcaacctg tataacgcgg tagtgaccgc agctgataaa gcctccgcca
    13381 accgtggcgt gctggtagtg atgaacgaca ccgtgcttga tggccgtgac gtcaccaaaa
    13441 ccaacaccac cgacgtagcg accttcaagt ctgttaacta cggtcctctg ggttacattc
    13501 acaacggtaa gattgactac cagcgtacac cggcacgtaa gcacaccagc gacacgccat
    13561 tcgatgtctc taagctgaat gaactgccga aagtcggcat tgtttataac tacgctaacg
    13621 catccgatct tccggctaaa gccctggtag atgcgggcta tgatggcatc gtgagcgctg
    13681 gtgtgggtaa cggcaacctg tataaatccg tgtttgacac gctggcgacc gccgcgaaaa
    13741 acggtactgc agtcgtgcgt tcttcccgcg taccgacggg cgctaccact caggatgccg
    13801 aagtggatga tgcgaaatac ggcttcgtcg cctctggcac gctgaacccg caaaaagcgc
    13861 gcgttctgct gcaactggct ctgacgcaaa ccaaagatcc gcagcagatc cagcagatct
    13921 tcaatcagta ctaatcacct cgccccggta tcgtgccggg gctttttcac ttcagactta
    13981 cgtccattgc caatttttat taccctgatg ataatcactg gaataaatta ttccgcgcga
    14041 gggttttcgg gtgaaaaagc aatggattgt tggcacggcg ctgcttatgt tgatgactgg
    14101 taatgtctgg gcagatggcg aaccgccaac tgaaaatatc ttaaaagatc aattcaaaaa
    14161 gcagtatcac ggcattctca aacttgatgc catcacctta aaaaatcttg atgctaaggg
    14221 taatcaggcc acctggtcag cggaaggcga tgtctcttcc agtgacgatc tctatacctg
    14281 ggtcggtcag ttggcagatt acgaactgct ggaacagacc tggacgaaag ataaaccggt
    14341 aaaattctcg gcgatgttaa ccagtaaagg aacgcccgcg tctggctgga cggtgaactt
    14401 ttactctttt caggcggcag ctagcgatcg tgggcgggtg gtcgacgata tcaaaacgaa
    14461 taataaatat atgatcgtga atagcgaaga tttcaattat cgctttagtc agcttgagac
    14521 tgcgttgaat acccagaaga actcgattcc tgcgctggaa aaggaagtga aagcgcttga
    14581 taagcaaatg gtggcggcgc agaaagcagc ggatgcatat tggggaaaag atgcaaatgg
    14641 taagcaaatg acccgcgaag acgcctttaa aaaaattcac caacagcgtg atgagtttaa
    14701 taaacagaac gatagcgagg cgttcgcggt taaatatgac aaagaggttt atcaaccggc
    14761 gattgcggca tgtcataaac agagtgaaga gtgttatgaa gtgccgattc agcagaaacg
    14821 agatttcgat atcaacgaac aacggcgaca gacttttctg caatcacaaa aacttagccg
    14881 taaattgcag gatgactgga taacgctcga aaaagggcaa tatccgttaa cgatgaaagt
    14941 ttcagaaata aacagtaaaa aagtcgcgat tttgatgaag attgacgata tcaataaggc
    15001 ctgcgatcgc tggaaaaaag ataccgaaca actgcggcgc aatggggtga ttaagtaagt
    15061 tttgatatag cgcacgattt gtagcaggat gcggcgttga ccccgcatcc ggcaatcaac
    15121 gtctgatgcg acgctagcgc gtcttatcag acctacaacc gttgccacat gtaggctgga
    15181 taaggcgttc acgccgcatc cggcaataca agtttactca gccagaaaaa gctccagcag
    15241 cgaatttaaa aacaacttcc catgttccgt tatctgccag taatccgcac attctgtgag
    15301 ataaccctgg gcaatcgcct cgtctaactg tgggcgaatc acatcttcgc aaagcccggt
    15361 atacgcacta aactctgcgc gtggcgcggc ctccagcaag cgaaagcggt tcataaagaa
    15421 ctcaaacggc ttatctgcag cttcgacatc acgctggctt tccagatacc ttccctgcat
    15481 aaaaccacgc ggatgacgcg ttttggtggt acgcagaatg cgcccatccg ggaaggtcac
    15541 tttgccgtgc gcgccgcagc caataccgat gtagtcgcca aatcgccagt agttaagatt
    15601 gtgctggcac tgataacccg gtttggcgta agcggaagtt tcatattgct gatagcccgc
    15661 tgcggttaat aactgatgcc cctgttcgaa aatatcccac agcgcgtcat cgtccggcag
    15721 caccggtggt cgcgaaccaa acagcgtatt gggttcgatg gtcagttgat accaggaaag
    15781 atgcggcgga ttcagttcaa tggcctggcg cagatcgcca agcgcctctt ccagtgattg
    15841 atccggcagc ccatgcatca aatcaaggtt aaaactacgc agccccagcc cgcttgccag
    15901 attcgcagct cgtttcgctt cttgcgggcc atgaatgcgc ccaagtcgtt tcagcttttc
    15961 ttcgctaaaa ctctgtacgc caatagagat gcggttcacg ccagcacgct gataatcgac
    16021 aaagcgatcg gcttctaccg taccagggtt cgcttccata gtaatttctg catccgctgc
    16081 cagcggcaaa cgcgcacgca cgccgtccag cagcgtttgc atcgccgggc cggaaagcag
    16141 gctcggcgta ccaccgccaa taaaaattgt ctgcacttca cggccctgag cgtaagccac
    16201 atcattgtcc agatcgttaa gcagatgctg aacataatcg tcgtgcggca cttctccttt
    16261 caacgcgtga gagttgaaat cgcagtacgg gcatttctgc acgcaccacg ggatgtgaat
    16321 gtagagactc agcggcggta atttaaccat tacgtaaagc gtccagcagc agtttcaacg
    16381 cctgaccacg gtgggaaatg gcgctctttt gttcgcgagt cagttcggca gcggttttcc
    16441 cttcggaagg tacgaagaag attggatcat aaccaaagcc accagtgccc gccggttcac
    16501 gagtaatcac tcctggccag ctaccgtggc acaccagcgg agtcggatct tccgcgtgac
    16561 gcagatacac cagcacgcag tggaaacgcg cctgacgttg gtcgtccggt acgtctttca
    16621 gtgtttccag cagtttttgc agattctttt gatcggtcgc gtcttcaccg gaataacgcg
    16681 cggagtaaat gcctggcgcg ccaccaagta catctaccgc cagaccggag tcgtcggcaa
    16741 ttgcaggtaa accggtcact tttgccgcat ggcgcgcttt cagaatcgcg ttttcgataa
    16801 aggtcaggcc ggtttcttca gcggaatcta cgccgaggtc tgtttgggcc acgatatcaa
    16861 gaccgaagtc gctaagcagc gacgccagct cacgcacttt accggcattg ccggttgcga
    16921 ggacaacttt ttgcatagga tacctaatta attaacgccg cgatttctgg cgggatttgt
    16981 tgcggattaa tgattttaat ttgtttgtgg cggccaagtt cgcctttttc aatcaccacc
    17041 tggcttttgg caacccggaa ttgcttaccg agaaacttca ccagatgact gttggcctgg
    17101 ccgtcaaccg gcggcgcggt tatggcgact tttacctcgt cgccatgtaa accgacaata
    17161 gaatcacggc tggctttcgg ctgaatataa agccgtaaaa ccagaccgtc atcgttaact
    17221 gttacggcac tcataacgcc atccacagcc ccggcagcag catgtttccg gttgcctgta
    17281 atacttctgc gacacccata ttgatgacat acagcagcag aacgaggatc atcggcgaga
    17341 aatcaatccc acccattgcc ggtagcaggc ggcgaatcgg gcgcagcagc ggatcggcca
    17401 gctgaatcag cacgtattca atcgggctac ggccctggct tacccagctc ataatcgcca
    17461 tcaccagcag gacccagaaa atcagcaggc cgatggtttt cagcagaatc agtaaaccgg
    17521 caatccagat gattggcagg aaggtcacca ctttaaacag cacgatggct ttgataaaac
    17581 tgagaatata ggcaaccagc agcgaggcgc tgtcaatggg gcccattgcc ggaataacgc
    17641 ggcgcagtgg cccgataatt ggctgcgtta ccttcactac aaactgtgag aaggggttgt
    17701 aaaaatcaca atgagcccac tgcatccaga tgcgtaataa cagcaccatg gtatacagct
    17761 caatgaccgt tgaaagcagg aaagtcaacg tattcatggc gttcctttaa ttccttattt
    17821 tttagagtaa tcacgcgcac caaaaattgc agtgccgata cgaaccatcg tgctacctgc
    17881 cgcaatggcg gcttccatat cgtccgacat tcccagagag agcgtgtcga tatgcgggta
    17941 gcgcgttttc agtccggcaa atgctacagc catttggcgt gcaacttcaa actgccttac
    18001 atattctgac tcaggcgcag ggattgccat cagcccgcgc agacgtaaac gcggtagttc
    18061 agcgaccgct gctgccagct cgtccagttc tgccagttga atcccggact tactgttttc
    18121 atcactaata ttaatttgaa tcagcacgtt aagaggggga agttctgccg ggcgctgatc
    18181 gttgaggcgg gtagcgatgc gcaaacggtc gatggtatga caccagtcga aatgctctgc
    18241 caccaggcgg cttttattag actgcaacgg gccaataaaa tgccattcta atcctgttac
    18301 gcccagttcc tgaaagtggc gaattttatc taccccttcc tgaacgtagt tttcaccaaa
    18361 ttgacgctgc ccggcatcaa tggcttctgc gatggcgctc gcaggttttg ttttactgac
    18421 tgcaagcagc gtaatttctt ctggagaacg gccgcaacgc gttgcagccg ctgagatttt
    18481 gtcccggacc tgtgccaggt tatgcgcaat atcgttcatt ttccgaggat cttagtatga
    18541 atatggaaga aattgtggcc cttagtgtaa agcataacgt ctcggatcta cacctgtgca
    18601 gcgcctggcc cgcacgatgg cgcattcgcg ggctaatgga agctgcgccg tttgatgcgc
    18661 cggacgtcga agagctactg cgggagtggc tggatgacga tcagcgggca atattgctgg
    18721 agaatggtca gctggatttt gccgtgtcgc tgacggaaaa ccagcgattg cgtggcagtg
    18781 cgttcgcgca acggcaaggt atttctctgg cgttacggct gttaccttcg cactgcccgc
    18841 agctcgaaca gcttggcgca ccaccggtat tgccggaatt actcaagagc gagaatggcc
    18901 tgattctggt gacgggggct acggggagtg gaaaatctac cacgctggcg gcgatggttg
    18961 gttatctcaa tcaacatgct gatgcgcata ttctgacgct ggaagatcct gtggaatatc
    19021 tctatgccag ccagcgatgt ttgatccagc agcgggaaat cggtttgaat tgtatgacgt
    19081 tcgcattggg attgcgggcc gcattgcggg aagatcctga tgtgattttg ctcggagagc
    19141 tgcgtgacag cgagacaatc cgtctggcgc tgacggcggc agaaaccggg catttggtgc
    19201 tggcaacatt acatacgcgt ggtgcggcgc aggcagttga gcggctggtg gattcatttc
    19261 cggcgcagga aaaagatccc gtacgtaatc aactggcagg tagtttacgg gcagtgttgt
    19321 cacaaaagct ggaagtggat aaacaggaag gacgcgtggc gctgtttgaa ttgctgatta
    19381 acacacccgc ggtggggaat ttgattcgcg aagggaaaac ccaccagtta ccgcatgtta
    19441 ttcaaaccgg gcagcaggtg gggatgataa cgtttcagca gagttatcag cagcgggtgg
    19501 gggaagggcg tttgtgacgt tgccatgccc gatgtgcaag cttatcgggc ctacaaaggc
    19561 aataaacgtc tgatgcgacg cttgcgcgtc ttatcagacc tacaaccgct gccacatgta
    19621 ggccggataa ggcgttcacg ccgcatccgg caataaggcg taaggcgcga tgatattatt
    19681 tacactccag caggcgttta aatcgcctta atatccctgc tcgaaatagc tttcgagaat
    19741 aataaccgca gaggcagagt cgactttgcc tttgttgagc gcccgatagc cgccctgttc
    19801 aaacagaccg gaacgggctt ccacggtgct aagacgttcg tcatggagct ttacttcaac
    19861 accgaaacga ccatgaatac ggttggcaaa tttacgcgct ctggcagtca atggttgctc
    19921 ggtaccgtcc atattcagcg gcaaaccgac gatgatttcg tccggctgcc actctttcag
    19981 taaacgttcg atgaggttcc agtccggcgt accgtcctgt gctttaattg caggcaaagg
    20041 gcgagcggtg ccggtaatgc gttggccgac cgctacgcca atgcttttgg tgccgaagtc
    20101 gaaggcaagt aaggttccac tcatcaggcg tgccctgcca caccaggcat ggtgagaata
    20161 tccacaccaa tcagttttgc cgcctcgcgc cagcggtcgg caattggcgt tttgaacaga
    20221 atattcagat ctgccggggc ggttagccac gcgttatcga gaatttcttg ttccagttga
    20281 cctttttccc aggaggcata gcccagagcc accaatacgt cagacggttg tttatcggtg
    20341 ccgagcgttt ccagcacatc gcgggaagtg gtcatcaccg tgttgtctga aatgcgaatg
    20401 ctggaagcaa aattggaggg cggagtatgc aaaataaacc cgcgatcttc agccagtggg
    20461 ccgccgagca taaccggttt atccagacgg attgattcat cacgcggctc cggcgtgatc
    20521 ttcagctttt ccagaatccc ttcaattttg agattttcca gcggcttgtt gacgatgatc
    20581 cccattgcac cattggtatt atgttcgcaa atgtagacca cggaacgacg gaaaatcgga
    20641 tcctggagag caggcatggc aataagaaag tgatgctgta aattcattgt cagaggttct
    20701 gtttcctggt tcaaaaaagc aacagcgccc agtatgcgtg gaaagaaggg cgctgtcact
    20761 cagagtctca acgagatcct tctcgctaag gtgggttact gctgctgtaa acgtgcttcg
    20821 atggcatcca ttaacattcc ggtgatcgac accggaaact ctgcttcaat ctcacgaata
    20881 caggttgggc tggtgacgtt aatttcagtc agacggtcgc cgatgatatc cagaccaaca
    20941 aaaatcagcc ctttttcttt cagcgtcggc ccgatctgac gggcgatttt ccagtcactt
    21001 tccgtcagcg gacgaggttc accgcgacca ccggcagcca gattgccacg ggtttcgccc
    21061 ccctgcggaa tacgcgccag gcagtacggt accggctcgc catccaccac cagcacgcgt
    21121 ttgtcgccat ctttaatggc tggcaggtaa ttttgcgcca tgcagtagcg agtgccatgc
    21181 tcagtcaggg tttcggcaat cacgccgagg tttggatcgc cttctttcac gcggaaaatc
    21241 gacgcgccgc ccataccgtc cagcggctta agaatgatgt cgctgtgttt ctcccagaac
    21301 gcttttagct gcgctttatt gcgcgtaacc agcgtttctg gcgttaagtc agagaaccag
    21361 gcggtaaaca gtttctcgtt acagtcgcgc aggctctgcg gcttgttaac gatcagcgtc
    21421 cctttctctt cggcacgttc cagaatatag gtcgcgtaga taaactcggt atcaaacggc
    21481 gggtctttac gcatcaggat cacatcgaga tcggccagcg gcagatcctg ttcaccgacg
    21541 aatgaaaacc actcttcgta gttctgcttc acgttcagcg tgcgggtatg ggcgcgggct
    21601 tcgccattga tcagatacag atcgcccatc tccatatagt gaagttcgta accacgacgc
    21661 tgtgcttcca gcaacatagc gaaactggaa tctttcttga tgttgatgtt tgcgatgggg
    21721 tccatcacga tgccgagctt gatcattatt cttctccgtt agcccaaatc gccaaatcgt
    21781 acttgtagcg cggtaatggc ggtgagcgca gttgtctctg tacgcaaaac gcgaggtccc
    21841 aacaggatat cagtaaattg atagcgggca gtcatggcaa tttcatctgc cgataaaccg
    21901 ccttccgggc caatcagcag gcggacgcgt tcaaccggta acggcaacgt attgatgctg
    21961 ttactggcgc gcgggtgaag attcagtttc agtccttcat cctgctctgc acaccaggct
    22021 tccagatcca tcgctggacg gatttccggc acccggttac gaccacactg ctcacaggca
    22081 gcaattgcaa tcttctgcca ctgctggagc ttcttgttca gacgttcact atccagttta
    22141 acgccgcagc gctcagaaaa aagtggcgta atgaggctta caccgagttc gatcgatttc
    22201 tggatcgtaa attccatttt ttcaccacgc gacatcacct gaccgaggtg aatatgcagc
    22261 ggagattcgc gatcgtcgat ctggccttcc agcaccttca cttccacgct ttttttgctg
    22321 gcgctggtaa tttcggcgtc aaagacctgg ttgctgccgt caaacaattg caacgcctgc
    22381 cccggcccca tgcgcagtac gcgcccgata tggttggcgg catcttcgca aagcgcgatg
    22441 tgagaatggc tggtcagtgg ttcaggatga taaatgcggg ggatacgcat agttaaaaat
    22501 ccgcgtcgtc tccccacgcg gttgttcgcg tggggtaggg gttaacaaaa agaatcccgc
    22561 tagtgtaggt tagctctttc gcgcctggca agcgcgttgc acatacgggt tatgattgcc
    22621 ctgcaccttc gcgatgcgtt catcgcgctc gcactcccag tcggtaaccg gatacatctt
    22681 gttccatgcg ttgaacagct gcgtttgctg gcgagagagt gtcaggttgt attggtcacg
    22741 catatagaag taggtgcgcg caatggcacc gcgtgcacgc gctggtggtt cggcagcttt
    22801 ttctttgaaa tcgaccttca tggcgcattg accgtactgg ccttcgccgc cattccactg
    22861 gctgtacata aagttgccgc gatcgccatt cacctcgccg actgacggct gcaggttatg
    22921 catatcgctt tccatcttgc gatagaccgg atctttagcg cagtttttac gtccaccgtc
    22981 ctgccagcac tggcgctggt gaccgaattg ccaggcggga acgacgtgtt cccactctac
    23041 gcggctggcg cggttttcat ttttgcgcac ctgatagccg cacgattgca gatcaacaac
    23101 gccttttttg ccctgccagt taattttaca tccgcaataa aacgtaccgg gcgcgtcagc
    23161 gtggactttt accgccgcgg ctttcgcctg agaaaaacta ttgatacctt cggccaacgc
    23221 cgggccggaa aatgctgcgc tcagtaccac cgcagcaata gacaaataac ggtacatcac
    23281 ttactccgtg ttaaaacgag ccagcaacgt agcgaaagtt gttttggtat gcaatcagcc
    23341 agatcaggaa agttcctgat agttcagatg gttattttgc gaccagttgt tcgccgcagt
    23401 gaacacagcg atagacggct tcgccacgaa ctacgcgatt atggcggcgt acggtaagct
    23461 gatgctcctg gcacttgcag cggtagggga aggtgttgcg acgcacggat tgcagttcga
    23521 actgatgcgt acgacgggcg ggaacaccca gcacgttttc catcatccac ttccactctt
    23581 tgccatgtgg tgctacgcgc ccgaaatgtt tccataccag caaatgtgcc agttcgtgcg
    23641 gcaccacttc ttcaataaag gcttcactgt tttccaacag caaaacggga ttgaggcgga
    23701 tttcatagct ttccagccag gctgttccgg cggaggtgcc gcgctgggtg taagagagtt
    23761 ttggctccgg gtagttacgc cccaatttca ggttagcctg ggcgagtttt tcccgcaggc
    23821 gacgcataac ggcctgttgg atggcgatag ggagacggga tgttttcata gcggcagagg
    23881 atagagcgaa gaaaagatga ctgcaagagg cggcttcctc cgcgacggga ggaagcctga
    23941 ggagattaat cgtgagcgcc gatttcgcgc agtttacgac ctttcatcag attacgttca
    24001 atatgttcca gcgaaacgtg tttggtttcc ggtaccagcc acaatgtcag caggataaac
    24061 agtacgttca gagccgcgta cacccagaag gtgttggcgt tacccagcgt gttgagcatg
    24121 gtcaggaacg ttgcgccaac gatcatgttg gcaatccagt tggtggcggt ggagcaggtg
    24181 atgccaaaat cgcggccttt cagcggctga atttcggagc acagtaccca aatcagcgga
    24241 ccggcactca tggcaaaacc gacaataaac atcagcagca tggcgatggc gaaatactgc
    24301 gccgacggag agtggatacc gatatgcatc attgtaccga gtacgcccat accagcagcc
    24361 atcaccagga agcccagcgt tagcgttggt ttacgtcccc agcggtcaac aaggccgatt
    24421 gcgataaagg tggcaagtac gttggtcagg ccgacaatca ctgtccccca catttgctcg
    24481 gtggtgttgg tataacccgc cagttcgaag attttcggcg cgtaatacat gatgacgttc
    24541 atcccggtga attgctgcat tacctgtaac agtacgccaa ggaacaccgc gcggcggaag
    24601 ttgctattct ctttaaacag cgcccagcca ctctgtttaa cctgcaaact ttcacggatt
    24661 tcatccagtt cgcgtttcgc ttccgcgctg gtgtcacgca ggcgtagcag cacgcgttcg
    24721 gcatcaacaa aacggcgttt ggcggcaaac caacgtgggc tgtctggcag gaagaagaca
    24781 ccaatcagca gcaaaattgc cgggatgata atcacaccca gcatccagcg ccatgcaccg
    24841 gtgtagctga aggcagtatc agaaagataa gcaccgagga tcccgatagt gatcatcaac
    24901 tgatacatcg agatcatact gccgcgaatt ttttccggcg caatttcaga gaggtacagt
    24961 ggtgcggtat aagaggccac accaaccgcc agccccagca gaacgcggga aagaatcagt
    25021 acttcaacgt ttggcgcagc cgcagagaac agcgaaccgg caacaaacaa aattgcgccg
    25081 atcatcaggc tctttttgcg cccgagttta aaggagagcc agccgctgcc caccgcaccg
    25141 actgccgcac cgaacatcat ggagcttacg acccattctt gcgtgtgcga agtaatctgg
    25201 aattcatctg caataaacgg cagtgcgcca gcaattacac cgatatccag gccaaagagt
    25261 aatcccgcca gagcggcaag gaagcagacg aaaaacgtca ttgccttgtt tgaccgcccc
    25321 tgttttttag cgtcaggcat gatgccctcc aatatggtta ttttttattg tgaattaaga
    25381 taggtgagta cgacgtaaaa agatgtgaag caaatcacat cagtgtaatc gcttacacta
    25441 acatttatct tagttatgat ttaagtgcat gaaaataatt gagttatatc atcatagatt
    25501 aaacgctgtt atctgcaatt aagactttac tgaaaagaaa tgtaacaact gtgaaaaccg
    25561 aataataata tgaaaatatt acaaatcttt gtctgttgtg gttggtgtaa tcggtttcag
    25621 ttgtgatgat aatttgcggt gaaacgtgat caggaagaat gatgttattg tgcaggcggt
    25681 ttttttgccg gatgcggcgt gaacgcctat ccaccgcggt gaggtaaaag aaaaaggcca
    25741 gcgcgaggct ggcctttgaa agcaggtgaa gaaagattac ttcagaccgg cagcatcgcg
    25801 cagcagctgc gctttgtcgg ttttttccca cgggaaatgt tcacgaccaa agtgaccgta
    25861 tgctgcggtt tctttgtaga tcgggtgcag cagatccagc atctgaatca gaccgtatgg
    25921 gcgcaggtcg aagaactcac gtaccagcag agtcagttgt tcagaaggca ctttctcggt
    25981 accgaaagtt tctaccatga tggaagtcgg ttcagccacg ccgattgcgt aggaaacctg
    26041 aatttcacaa cgatcggcca ggccagcagc aacgatgttt tttgcaacat aacgtgctgc
    26101 gtacgcagcg gaacggtcca cttttgatgg atctttacca gagaatgctc cgccaccgtg
    26161 acgtgccatg ccgccgtagg tatcaacgat aattttacga ccagtcagac cgcagtcacc
    26221 catcgggcca ccgataacaa aacgaccggt cgggttgatg aagaatttgg tggcagaagt
    26281 cagccattca gcgggcagaa tcggcttgat gatctcttcc attaccgctt cttgcagcga
    26341 tttctggtcg atctcttcag agtgctgagt ggaaagcacg acagcatcga taccaacgat
    26401 tttgccgtcg tcatactgga aagtcacctg gcttttcgcg tccgggcgca gccacggcag
    26461 agtgccgttt ttacgcactt cagcctgacg ctgtaccaga cggtgtgcat aggtgatagg
    26521 tgctggcatc agcacgtcgg tttcattagt tgcgtagcca aacatcagac cctggtcacc
    26581 cgcgccctgt tccagcggat cggcacggtc aacgccctgg ttgatgtcag gagactgttt
    26641 gccgatagcg ctcagaaccg cacaagagtt agcgtcaaag cccatgtcgg aatgtacata
    26701 gccaatttcg cgaacggtgt tacgggtgat ctcttcgatg tctacccagg cgctggtggt
    26761 gatttcgccg ccaactaaaa ccatgccggt ttttacgtag gtttcgcaag caacgcgtgc
    26821 tttcggatcc tgttcgagga tcgcgtctaa aacggcatca gagatttggt cagcaatttt
    26881 gtcaggatgc ccttcagaga cggactcgga cgtaaaaagg tgttttgcca tatttaatat
    26941 cacctaaaga gaatttggtt agctcaaact gttgtgtgga ttttctgtgg tagcggatcc
    27001 taccacgact ctgcaggtta aaaacactgg cagtctgagt gttaatcggt atggatggat
    27061 taacatctgg atggctattt taggtcaatt cttcacccga tttccacttt tttttgaatc
    27121 gtgtctcatt ctgttaaaaa cgtggctgga aatttttcct gacaatgccg gcattctgcg
    27181 tatttatctt ttgcaatttt ctgccattgt ggggtataaa acgcggcgcg cggcttaaat
    27241 aaaaagcaca cgacgtttct ttcgtgttgc cacttccagc cgggttgaaa tcagagtttt
    27301 ggcttgtggg ttcgtcttaa caggcggccg tggaggtgat acgaaataat gaaccgttgt
    27361 ctgctgctta acctgtctca ccgttctggt gaagattcgt tccccgcact ctgcatctct
    27421 gctttgcata cctgccgatg ttatacccat ctcggcgctt ctcaggattc aagagctggt
    27481 tacagttact gaggactgaa caagggcgct cttgtaaaaa caagagtttt ctcgtggttt
    27541 cgccgaactg tcacacttac gttcggttat gtgcttaata atgttatgaa aaagaaaccg
    27601 gttgcgcagt tggagcgtca gcattcactg ctggaaaatc catgtgctta tgggttgtta
    27661 tcgcagttcc aggctgcgat agtcgttaac tgttttacac ttaataaaat aatttgaggt
    27721 tcgctatgtc tgacgacatg tctatgggtt tgccttcgtc agcgggcgaa cacggtgtac
    27781 tacgctccat gcaggaggtt gcaatgagct cccaggaagc cagcaagatg ctgcgtactt
    27841 acaatattgc ctggtggggc aataactact atgacgttaa cgagctgggc cacatcagcg
    27901 tgtgcccgga cccggacgtc ccggaagctc gcgtcgatct cgcgcagtta gtgaaaactc
    27961 gtgaagcaca gggccagcgt ctgcctgcac tgttctgttt cccacagatc ctgcagcacc
    28021 gtttgcgttc cattaacgcc gcgttcaaac gtgcgcggga atcctacggc tataacggcg
    28081 attacttcct tgtttatccg atcaaagtta accagcaccg tcgcgtgatt gagtccctga
    28141 ttcattcggg cgaaccgctg ggtctggaag ccggttccaa agccgagttg atggcagtgc
    28201 tggcacatgc tggcatgacc cgtagcgtca tcgtctgcaa cggttataaa gaccgcgaat
    28261 atatccgcct ggcattaatt ggcgagaaga tggggcacaa ggtctatctg gtcattgaga
    28321 agatgtcaga aatcgccatt gtgctggatg aagcagaacg tctgaatgtc gttcctcgtc
    28381 tgggcgtgcg tgcacgtctg gcttcgcagg gctcgggtaa atggcagtct tccggcgggg
    28441 aaaaatcgaa gttcggcctg gctgcgactc aggtactgca actggttgaa accctgcgtg
    28501 aagccgggcg tctcgacagc ctgcaactac tgcacttcca cctcggttcg cagatggcga
    28561 atattcgcga tatcgccacg ggcgttcgtg aatccgcgcg tttctatgtg gaactgcaca
    28621 agctgggcgt caatattcag tgcttcgacg tcggcggcgg tctgggcgtg gactatgaag
    28681 gcacccgttc gcagtctgac tgctccgtaa actatggcct caatgaatac gccaacaaca
    28741 tcatctgggc gattggtgat gcgtgtgaag aaaacggtct gccgcatccg acggtaatca
    28801 ctgaatcggg tcgtgcagtg actgcgcatc acaccgtgct ggtgtctaat atcatcggcg
    28861 tggaacgtaa cgaatacacg gtgccgaccg cgcctgcaga agatgcgccg cgcgcgctgc
    28921 aaagcatgtg ggaaacctgg caggagatgc acgaaccggg aactcgccgt tctctgcgtg
    28981 aatggttaca cgacagtcag atggatctgc acgacattca tatcggctac tcttccggca
    29041 cctttagcct gcaagaacgt gcatgggctg agcagcttta tttgagcatg tgccatgaag
    29101 tgcaaaagca gctggatccg caaaaccgtg ctcaccgtcc gattatcgac gagctgcagg
    29161 aacgtatggc ggacaaaatg tacgtcaact tctcgctgtt ccagtcgatg ccggatgcgt
    29221 gggggatcga ccagttgttc ccggttctgc cgctggaagg gctggatcaa gtgccggaac
    29281 gccgcgctgt gctgctggat attacctgtg actctgacgg tgctatcgac cactatatcg
    29341 atggtgatgg tattgccacg acaatgccta tgccggagta cgatccagag aatccgccga
    29401 tgctcggttt ctttatggtc ggcgcatatc aggagatcct cggcaacatg cacaacctgt
    29461 tcggtgatac cgaagcggtt gacgtgttcg tcttccctga cggtagtgta gaagtggaac
    29521 tgtctgacga aggcgatacc gtggcggaca tgctgcaata tgtacagctc gatccgaaaa
    29581 cgctgctaac tcagttccgt gatcaagtga agaaaaccga tcttgatgct gaactgcaac
    29641 aacagttcct tgaagagttc gaggcaggtt tgtacggtta tacctatctt gaagatgaat
    29701 aattaagatt attccgctgg cgggtcgatt tatttacgta aatcggcccg ctttcttttt
    29761 atatattctt aaaaataatt tagctgcttt tcttattaaa taatctccct acaatcgctg
    29821 cattaattaa atgcagaggg aacggttatg aaaaaatgga aagtacgtag cgcgttgata
    29881 gcattagtcg tgctgctggt cggttgttcc agtaatgcgc agtataatag cagtgcatcg
    29941 ggcaatgtcg gcaccgcctg gggtggggat gtgcactcat cggttcaggg cgtatctgct
    30001 gaacgagcct ggcgcgatcc tgcagagatg atcgttatta gttattcgac taatgttcct
    30061 tccggctacg accgcgttta tagcattcgt attaacgaac tggaatatgc tattcgcgat
    30121 ggtaatttta attctctacc tattacgcgg gcttatgatt catccaataa tgaaccacgc
    30181 tatattgtcc acgccagagt cggaatgaat tatcaattat atgttcgtaa ttacagtcgt
    30241 aacaccaact acgagatcgt tgctacggtt gatggcctgg atgtgctgaa cggcaagcaa
    30301 ggttcgctga ataacaacgg ctatattgtg aatgccggag attcgttagc gattaaagga
    30361 ttccgcaaag ataaacatac cgaagcggct tttcagttcg ccaacgttgt tgattcctac
    30421 gcagccaatt cggcgcaggg cgatgtgcgt aataccgggg taatcggctt tgccgccttt
    30481 gaattacaag gcccggcgca aaacgcgctg ccaccgtgct ctggacaagc ttttccagca
    30541 gataacaatg gctatgcgcc gccaccgtgt cggaaatagc aacctcgctt gatgcctcgc
    30601 ccatttaagg cgataattag cacaaacttg tgtttcgcaa atccaaccct tcctcgtcgg
    30661 gcctaacgac gcggaagggt ttttttatat cgactttgta ataggagtcc atccatgagc
    30721 accttaggtc atcaatacga taactcactg gtttccaatg cctttggttt tttacgcctg
    30781 ccgatgaact tccagccgta tgacagcgat gctgactggg tgattactgg tgtgccgttc
    30841 gatatggcca cttctggtcg tgcgggggga cgccacggcc cggcagctat ccgtcaggtt
    30901 tcgactaacc tggcctggga acacaaccgc ttcccgtgga atttcgacat gcgcgagcgt
    30961 ctgaacgtgg tggactgcgg cgatctggta tatgccttcg gcgatgcccg tgagatgagc
    31021 gaaaaactgc aggcacacgc cgagaagctg ctggctgccg gtaagcgtat gctctccttc
    31081 ggtggtgacc actttgttac gctgccgctg ctgcgtgctc atgcgaagca tttcggcaaa
    31141 atggcgctgg tacactttga cgcccacacc gatacctacg cgaacggttg tgaatttgac
    31201 cacggcacca tgttctatac cgcgccaaaa gaaggtttaa tcgacccgaa tcattccgtg
    31261 cagattggta ttcgtactga gttcgataaa gacaacggct ttaccgtgct ggacgcctgc
    31321 caggtgaacg atcgcagcgt ggatgacgtt atcgcccagg tgaaacaaat tgtgggtgat
    31381 atgccggttt acctgacctt tgatattgac tgcctggatc ctgcttttgc accaggcacc
    31441 ggtacgccgg tgattggcgg cctgacctcc gatcgcgcta ttaaactggt acgcggcctg
    31501 aaagatctca acatcgttgg gatggacgta gtggaagtgg ctccggcata cgatcagtcg
    31561 gaaatcaccg ctctggcagc ggcaacgctg gcgctggaaa tgctgtatat tcaggcggcg
    31621 aaaaagggcg agtaagcacc agatgcgatg cgcacgggta aaa
//
LOCUS       DAZFFG010000045        30804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFFG010000045 DAZFFG010000000
VERSION     DAZFFG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.045"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..81
                     /locus_tag="ACHJ7J_002413"
     CDS             <1..81
                     /locus_tag="ACHJ7J_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000636.1"
                     /translation="MDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            311..694
                     /gene="secE"
                     /locus_tag="ACHJ7J_002414"
     CDS             311..694
                     /gene="secE"
                     /locus_tag="ACHJ7J_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO5000637.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            696..1241
                     /gene="nusG"
                     /locus_tag="ACHJ7J_002415"
     CDS             696..1241
                     /gene="nusG"
                     /locus_tag="ACHJ7J_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO5000638.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1400..1828
                     /gene="rplK"
                     /locus_tag="ACHJ7J_002416"
     CDS             1400..1828
                     /gene="rplK"
                     /locus_tag="ACHJ7J_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO5000639.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1832..2536
                     /gene="rplA"
                     /locus_tag="ACHJ7J_002417"
     CDS             1832..2536
                     /gene="rplA"
                     /locus_tag="ACHJ7J_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO5000640.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2828..3325
                     /gene="rplJ"
                     /locus_tag="ACHJ7J_002418"
     CDS             2828..3325
                     /gene="rplJ"
                     /locus_tag="ACHJ7J_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO5000641.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3392..3757
                     /gene="rplL"
                     /locus_tag="ACHJ7J_002419"
     CDS             3392..3757
                     /gene="rplL"
                     /locus_tag="ACHJ7J_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO5000642.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4077..8105
                     /gene="rpoB"
                     /locus_tag="ACHJ7J_002420"
     CDS             4077..8105
                     /gene="rpoB"
                     /locus_tag="ACHJ7J_002420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO5000643.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8182..12405
                     /gene="rpoC"
                     /locus_tag="ACHJ7J_002421"
     CDS             8182..12405
                     /gene="rpoC"
                     /locus_tag="ACHJ7J_002421"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO5000644.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12618..13156
                     /gene="yjaZ"
                     /locus_tag="ACHJ7J_002422"
                     /pseudo
     CDS             12618..13156
                     /gene="yjaZ"
                     /locus_tag="ACHJ7J_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
     gene            complement(13566..14699)
                     /gene="thiH"
                     /locus_tag="ACHJ7J_002423"
     CDS             complement(13566..14699)
                     /gene="thiH"
                     /locus_tag="ACHJ7J_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO5000645.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETSDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14696..15466)
                     /gene="thiG"
                     /locus_tag="ACHJ7J_002424"
     CDS             complement(14696..15466)
                     /gene="thiG"
                     /locus_tag="ACHJ7J_002424"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO5000646.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAHEALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15468..15668)
                     /gene="thiS"
                     /locus_tag="ACHJ7J_002425"
     CDS             complement(15468..15668)
                     /gene="thiS"
                     /locus_tag="ACHJ7J_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO5000647.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15652..16407)
                     /gene="thiF"
                     /locus_tag="ACHJ7J_002426"
     CDS             complement(15652..16407)
                     /gene="thiF"
                     /locus_tag="ACHJ7J_002426"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO5000648.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16400..17035)
                     /gene="thiE"
                     /locus_tag="ACHJ7J_002427"
     CDS             complement(16400..17035)
                     /gene="thiE"
                     /locus_tag="ACHJ7J_002427"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO5000649.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17035..18930)
                     /gene="thiC"
                     /locus_tag="ACHJ7J_002428"
     CDS             complement(17035..18930)
                     /gene="thiC"
                     /locus_tag="ACHJ7J_002428"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO5000650.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19011..19111)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19163..19639)
                     /gene="rsd"
                     /locus_tag="ACHJ7J_002429"
     CDS             complement(19163..19639)
                     /gene="rsd"
                     /locus_tag="ACHJ7J_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO5000651.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19734..20507
                     /gene="nudC"
                     /locus_tag="ACHJ7J_002430"
     CDS             19734..20507
                     /gene="nudC"
                     /locus_tag="ACHJ7J_002430"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO5000652.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20547..21611
                     /gene="hemE"
                     /locus_tag="ACHJ7J_002431"
     CDS             20547..21611
                     /gene="hemE"
                     /locus_tag="ACHJ7J_002431"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO5000653.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGQVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21621..22292
                     /gene="nfi"
                     /locus_tag="ACHJ7J_002432"
     CDS             21621..22292
                     /gene="nfi"
                     /locus_tag="ACHJ7J_002432"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO5000654.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22335..22925
                     /gene="yjaG"
                     /locus_tag="ACHJ7J_002433"
     CDS             22335..22925
                     /gene="yjaG"
                     /locus_tag="ACHJ7J_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO5000655.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23112..23384
                     /gene="hupA"
                     /locus_tag="ACHJ7J_002434"
     CDS             23112..23384
                     /gene="hupA"
                     /locus_tag="ACHJ7J_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO5000656.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23397..24092
                     /gene="yjaH"
                     /locus_tag="ACHJ7J_002435"
     CDS             23397..24092
                     /gene="yjaH"
                     /locus_tag="ACHJ7J_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO5000657.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24094..24519)
                     /gene="zraP"
                     /locus_tag="ACHJ7J_002436"
     CDS             complement(24094..24519)
                     /gene="zraP"
                     /locus_tag="ACHJ7J_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO5000658.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24757..26133
                     /gene="zraS"
                     /locus_tag="ACHJ7J_002437"
     CDS             24757..26133
                     /gene="zraS"
                     /locus_tag="ACHJ7J_002437"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO5000659.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26130..27455
                     /gene="zraR"
                     /locus_tag="ACHJ7J_002438"
     CDS             26130..27455
                     /gene="zraR"
                     /locus_tag="ACHJ7J_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO5000660.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAGVKAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27452..28741)
                     /gene="purD"
                     /locus_tag="ACHJ7J_002439"
     CDS             complement(27452..28741)
                     /gene="purD"
                     /locus_tag="ACHJ7J_002439"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO5000661.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28753..30342)
                     /gene="purH"
                     /locus_tag="ACHJ7J_002440"
     CDS             complement(28753..30342)
                     /gene="purH"
                     /locus_tag="ACHJ7J_002440"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO5000662.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMIGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7254 a         7985 c         8422 g         7143 t
ORIGIN      
        1 atggacgacg gtctgcgttt cgcaatccgt gaaggcggcc gtaccgttgg cgcgggcgtt
       61 gtagcaaaag ttctgagcta attgccgata acatttgacg caatgcgcac taaaagggca
      121 tcatttgatg ccctttttgc acgctttcgt accagaacct ggctcatcag tgattttctt
      181 tgtcataatc attgctgaga caggctctgt tgagggcgta taatccgaaa agctaatacg
      241 cgtttcgatt tggtttgcct cgcgatcgcg gggtgaaaat gtttgtagaa tacttctgac
      301 aggttggttt atgagtgcga ataccgaagc tcaaggaagc gggcgcggcc tggaagcgat
      361 gaagtgggtc gttgtggtgg cattgctcct ggtggcgatt gtcggcaact acctttatcg
      421 cgacattatg ctgccgctgc gtgcgctggc cgtagtaatt ctgattgctg cagcgggtgg
      481 tgtcgcgctg ttaacgacaa aaggtaaagc taccgttgct tttgcccgtg aagcgcgtac
      541 cgaagtccgt aaggtcattt ggccgactcg ccaggaaaca ttgcacacca cgctgattgt
      601 ggctgcggtt accgcagtaa tgtcactgat cctgtgggga ctggatggta ttctggttcg
      661 cctggtatcc tttatcactg gcctgaggtt ctgagatgtc tgaagctcct aaaaagcgct
      721 ggtacgtcgt tcaggcgttt tccggttttg aaggccgcgt agcaacgtcg ctgcgtgagc
      781 atatcaaatt acacaacatg gaagatttgt ttggtgaagt catggtacca accgaagaag
      841 tggttgaaat ccgtggcggt cagcgtcgca aaagcgaacg taaattcttc cctggctacg
      901 tcctcgttca gatggtgatg aacgacgcga gctggcacct ggtgcgcagc gtaccgcgtg
      961 tgatgggctt catcggcggt acttccgatc gtcctgcgcc aatcagcgat aaagaagtcg
     1021 atgcgattat gaaccgcctg cagcaggttg gtgataagcc gcgtccgaaa acgctgtttg
     1081 aaccgggtga aatggtccgt gttaatgatg gtccgttcgc tgacttcaac ggtgttgttg
     1141 aagaagtgga ttacgagaaa tctcgtctga aagtgtctgt ttctatcttc ggtcgtgcga
     1201 ccccggtaga gctggacttc agccaggttg aaaaagccta acccagcgat caaaaaagcg
     1261 gcgatttaat cgttgcacaa ggcgcgagat tggaatacaa tttcgcgcct tttgttttta
     1321 tgggccttgc ccgtaaaacg attttttata tcacggggag cctctcagag gcgttattac
     1381 ccaacttgag gaatttataa tggctaagaa agtacaagcc tatgtcaagc tgcaggttgc
     1441 agctggtatg gctaacccga gtccgccagt aggtccggct ctgggtcagc agggcgtaaa
     1501 catcatggaa ttctgcaaag cgttcaacgc aaaaactgat tccatcgaaa aaggtctgcc
     1561 gattccggta gtaatcaccg tttacgctga ccgttctttc actttcgtta ccaagactcc
     1621 gccggcagca gttctgctga aaaaagcggc tggtatcaag tctggttccg gtaagccgaa
     1681 caaagacaaa gtgggtaaaa tttcccgcgc tcagctgcag gaaatcgcgc agaccaaagc
     1741 tgccgacatg actggtgccg acattgaagc gatgactcgc tccatcgaag gtactgcacg
     1801 ttccatgggc ctggtagtgg aggactaaga aatggctaaa ctgaccaagc gcatgcgtgt
     1861 tatccgcgag aaagttgatg caactaaaca gtacgacatc aacgaagcta tcgctctgct
     1921 gaaagagctg gcgactgcta aattcgtaga aagcgtggac gttgctgtta acctcggcat
     1981 cgacgctcgt aaatctgacc agaacgtacg tggtgcaact gtactgccgc acggtactgg
     2041 ccgttccgtt cgcgtagccg tatttaccca gggtgcaaac gctgaagctg ctaaagctgc
     2101 aggtgctgaa ctggtaggta tggaagatct ggctgaccag atcaagaaag gcgaaatgaa
     2161 ctttgacgtt gttattgctt ctccggatgc aatgcgcgtt gttggccagc tgggccaggt
     2221 tctgggtccg cgcggcctga tgccaaaccc gaaagtgggt actgtaacgc cgaacgttgc
     2281 tgaagcggtt aaaaacgcta aagctggcca ggttcgttac cgtaacgaca aaaacggcat
     2341 catccacacc accatcggta aagtggactt tgacgctgac aaactgaaag aaaacctgga
     2401 agctctgctg gttgcgctga aaaaagcaaa accgactcag gcgaaaggcg tgtacatcaa
     2461 gaaagttagc atctccacca ccatgggtgc aggtgttgca gttgaccagg ctggcctgag
     2521 cgcttctgta aactaatgcc tttacgtggg cggtgatttt gtctacaatc ttacccccac
     2581 gtatgctaac gaaagttagc agctaaaaag atttgttcgt tggagcctgg cctatccagg
     2641 cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta gacggtgaca
     2701 gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa ttaagacgct
     2761 ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac atccaggagc
     2821 aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag tcagcgaagt
     2881 agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg tagataaaat
     2941 gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg ttcgtaacac
     3001 cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg cgtttgttgg
     3061 tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc tgttcaaaga
     3121 gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag gtgagctgat
     3181 cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag caattgcacg
     3241 cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc tggctgctgt
     3301 acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc gcttacgtat
     3361 aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc aaatcattga
     3421 agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa tggaagaaaa
     3481 attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg aagctgctga
     3541 agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag ttgctgttat
     3601 caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc tggtagaatc
     3661 tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac tgaaaaaagc
     3721 tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg gttgcagcct
     3781 gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg ctgtaaggcg
     3841 ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt ttctatcgac
     3901 gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa tggtttaagc
     3961 gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa tagtgttgca
     4021 caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg aaccctatgg
     4081 tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt ccacaagttc
     4141 tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt atcgagcaag
     4201 atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg attcagagct
     4261 acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg gtgtttgacg
     4321 tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt aaactgcgtc
     4381 tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa gaacaagaag
     4441 tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc aacggtactg
     4501 agcgtgttat tgtttcccag ctgcaccgta gtccgggcgt cttctttgac tccgacaaag
     4561 gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct taccgtggtt
     4621 cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc gaccgtcgcc
     4681 gtaaactgcc tgcgaccatc attctgcgtg ctctgaacta cactacagag cagatcctcg
     4741 acctgttctt tgaaaaagtt atctttgaaa tccgcgataa caagctgcag atggaactgg
     4801 tgccggaacg cctgcgtggt gaaaccgcat ctttcgacat cgaagctaac ggtaaagtgt
     4861 acgtagaaaa aggccgccgt attactgcgc gccacattcg ccagctggaa aaagacgacg
     4921 tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct aaagactata
     4981 ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc ctggatctgc
     5041 tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc aacgacctgg
     5101 atcacggccc gtatatctct gaaaccttac gtgtcgaccc aactaacgac cgtctgagcg
     5161 cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt gaagcagctg
     5221 aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacctgtct gcggttggtc
     5281 gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt atcctgagca
     5341 aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt aaaggcgaag
     5401 tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc tgttggcgaa atggcggaaa
     5461 accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt ctgtctctgg
     5521 gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccaatt tccgcagcag
     5581 tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac aacccgctgt
     5641 ctgagattac gcataaacgt cgtatctccg cactcggccc aggtggtctg acccgtgaac
     5701 gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta tgtccaatcg
     5761 aaacccctga aggtccgaac atcggtctga tcaactccct gtctgtatac gcgcagacta
     5821 acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt gtaactgacg
     5881 aaattcacta cctgtctgct atcgaagaag gcaactacgt tatcgcccag gcgaactcca
     5941 acctggatga agaaggccac ttcgtagaag acctggtaac ttgccgtagc aaaggcgaat
     6001 ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag caggtggtat
     6061 ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt gcattgatgg
     6121 gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgataagccg ctggttggta
     6181 ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct aaacgtggtg
     6241 gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa gacgagatgt
     6301 atccgggtga agcaggtatc gacatctaca acctgaccaa atacacccgt tctaaccaga
     6361 acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa cgtggcgacg
     6421 tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag aacatgcgcg
     6481 tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta tccgagcgtg
     6541 ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt gtgtcccgtg
     6601 acaccaagct ggggccggaa gagatcaccg ctgacatccc gaacgtgggt gaagctgcgc
     6661 tctccaaact ggatgaatct ggtatcgttt acattggtgc ggaagtgacc ggtggcgaca
     6721 ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa gaaaaactgc
     6781 tgcgtgcgat cttcggtgag aaagcctctg acgttaaaga ctcttctctg cgcgtaccaa
     6841 acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc gtagaaaaag
     6901 acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa gacctgtctg
     6961 aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg ttggtagccg
     7021 gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg gagctgggcc
     7081 tgaccgacga agagaaacaa aatcagctgg aacagctggc tgagcagtat gacgaactga
     7141 aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag ggcgacgatc
     7201 tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc cgtatccagc
     7261 ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag atcaacccga
     7321 tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg aacccgctgg
     7381 gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacttgggt atggctgcga
     7441 aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaggaagtc gcgaaactgc
     7501 gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa gttgacctga
     7561 gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa ggtatgccaa
     7621 tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg ctgaaacttg
     7681 gcgacctgcc gacttctggt cagatccgcc tgtacgacgg ccgcactggt gaacagttcg
     7741 aacgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg gtcgacgaca
     7801 agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg ctgggtggta
     7861 aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg gaagcatacg
     7921 gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg aacggtcgta
     7981 ctaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc atgccagaat
     8041 ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa ctggaagacg
     8101 agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc ggattgtgct
     8161 aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc gcagactaaa
     8221 accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat ccgttcatgg
     8281 tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa accagaacgt
     8341 gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg cctgtgcggt
     8401 aagtacaagc gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt tgaagtgacc
     8461 cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc gactgcgcac
     8521 atctggttcc tgaaatcgct gccgtcccgt atcggcctac tgctcgatat gccgctgcgc
     8581 gatattgaac gcgtactgta cttcgaatcc tatgtggtta tcgaaggcgg tatgaccaac
     8641 ctggaacgtc agcagatcct gactgaagag cagtatctgg acgcgctgga agagttcggt
     8701 gacgaattcg acgcgaaaat gggtgcggaa gcaatccagg ccctgctgaa gagcatggat
     8761 ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc cgaaaccaag
     8821 cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc tggtaacaaa
     8881 ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg tccgctggtt
     8941 ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg tcgcgtcatt
     9001 aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat catcgtacgt
     9061 aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg tcgtcgcggt
     9121 cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat gatcaaaggt
     9181 aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc cggtcgttct
     9241 gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa gaaaatggcg
     9301 ctggagctgt tcaaaccgtt catctacggc aagcttgaac tgcgtggtct tgctaccacc
     9361 attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga tatcctggac
     9421 gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca ccgtctgggt
     9481 atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca cccgctggtt
     9541 tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt accgctgacg
     9601 ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat cctgtccccg
     9661 gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct gtactacatg
     9721 acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc gaaagaagca
     9781 gaacgtctgt atcgctctgg cctggcttct ctgcatgcgc gcgttaaagt gcgtatcacc
     9841 gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa agacacgact
     9901 gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc catcgtcaac
     9961 caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg cattctcggt
    10021 ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggctttgc ctatgcagcg
    10081 cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa acacgaaatc
    10141 atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc tggtctggta
    10201 actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa cgatcgtgta
    10261 tccaaagcga tgatggataa cctgcaaact gaaaccgtta ttaaccgtga cggtcaggaa
    10321 gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc gcgtggttct
    10381 gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc ggatggctcc
    10441 atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact ccagtacttc
    10501 atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac ggcgaactcc
    10561 ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac cgaagacgat
    10621 tgtggtaccc atgaaggtat catgatgact ccggttatcg agggtggtga cgttaaagag
    10681 ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa gccgggtact
    10741 gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga cctgctggaa
    10801 gagaactctg tcgacgcggt taaagtacgt tctgttgtat cttgtgacac cgactttggt
    10861 gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa caaaggtgaa
    10921 gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct gaccatgcgt
    10981 acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat ccaggtgaaa
    11041 aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag cggtaaactg
    11101 gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg taccaaagaa
    11161 agctacaaag taccttacgg tgcggtactg gcgaaaggcg atggcgaaca ggttgctggc
    11221 ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga agtaagcggt
    11281 tttgtacgct ttactgacat gatcgacggc cagaccatta cgcgtcagac cgacgaattg
    11341 accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg tggtaaagat
    11401 ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat ccctggtacc
    11461 gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga agatggcgta
    11521 cagatcagct ctggtgacac cctggcgcgt attccgcagg aatccggcgg taccaaggac
    11581 atcaccggtg gtctgccgcg cgttgcggac ctgttcgaag cacgtcgtcc gaaagagccg
    11641 gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa aggtaaacgt
    11701 cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat tccgaaatgg
    11761 cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat ttccgacggt
    11821 ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac tcgttacatc
    11881 gttaacgaag tacaggacgt ataccgtctg cagggcgtta agattaacga taaacacatc
    11941 gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg cagctccgac
    12001 ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg cgaactggaa
    12061 gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac caaagcgtct
    12121 ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg cgtgctgacc
    12181 gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa cgttatcgtg
    12241 ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat gcgtcgccgt
    12301 gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc tgccagcctg
    12361 gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt aatccgcaaa
    12421 taacgtaaaa acccgcttcg gcgggttttt ttatgggggg agtttaggga aagagcattt
    12481 gtcagaatat ttaatgaatt tctggatact cataatcaat gtagagatga ctaatatcct
    12541 gaaactgact gaactaattg agtcaaactc ggcaaggatt cgatactatt cctgtgtaac
    12601 tttcttaagg aacgagaatg aaacaggaag tggaaaagtg gcgacctttt ggacatccgg
    12661 atggtgatat cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12721 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12781 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12841 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12901 tattttggcg ctgccagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    12961 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13021 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttaa cccatttctg aaaaacagaa
    13081 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13141 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13201 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13261 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13321 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13381 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13441 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13501 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg tccttacatt
    13561 ccgtctcata gtctttgcga ggcgcgtccc agatagctgt cccagtcttt ccataccggc
    13621 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13681 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13741 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13801 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13861 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13921 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    13981 gcaaccatat aacagtcaac tcgccagttg tcggaaaggc caattagtgc gccgaggcca
    14041 atcttatcaa tccctgcacg ccccagccga tccgacgttt ccagccgcca gaagaagtcc
    14101 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14161 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctc cgccagcggt
    14221 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14281 taatccatcc ccactttcgc ctgatgttca ccggtgacta aaagcagatg ttcaaagccc
    14341 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14401 atgcggttgc tcatagagaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14461 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14521 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14581 tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt tttgccgttg
    14641 atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc gatcgctgaa ggttttcata
    14701 ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg agcaaaataa ctgcggctgc
    14761 ccggtccgga ctgacgtgcc agcaggcctg cttctactgc cagacgaaat gccttcgcca
    14821 tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14881 tttccagcgc ctgcgcggca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    14941 ctgtggcctg ctggataata atctccagca tggcgcgggt ttccagcccc tgattcgagc
    15001 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15061 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcgtttcgg
    15121 cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15181 attttaacca gtttgtgcct aacgcttcat gagccagatg ggcggcgaaa atggcttctt
    15241 ccgctgtttt cgccccggat gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15301 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15361 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15421 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15481 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    15541 cgtgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15601 tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca tcggttgatc gttaaacagg
    15661 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15721 ccactggctc gacttaccgt cgaacagtcg gagttctccc gcaggcgtct ctataccgct
    15781 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15841 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15901 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    15961 ggcggtgata agcggcgtgt tgagtgccac gcaggcggca ttaatctcct ggcgagtcgc
    16021 catattgtcg gtacagtcga gcaccacatc ggcttgtgca accgcatctt ttaacgcctc
    16081 acccgttaac cgttgttgta atgctgtcag ttgaatatcg ggattcaact gtgtcagtcg
    16141 ctgatggctg acctgcgatt tcgggcgatc gatatcttca gtggtaaaga ggatttgtcg
    16201 ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16261 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16321 gctgtcgagc agtttttgct gcccgtcaag agcgatatcg tcgagcagga tttggcggct
    16381 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16441 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac gacggcgata ctgccgacac
    16501 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16561 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16621 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16681 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacgcc cagccgcagg cctgccgcgc
    16741 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16801 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16861 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16921 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    16981 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17041 ttcctcctta cgcaggtaga tttctccgcc tctggcgcgg aagttttccg acatatccgc
    17101 cattcccact tcaatggttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17161 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17221 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17281 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17341 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17401 aacatcttct ttattaggca gacccagatg ctcttttggc gttacgtaac agagcatcgc
    17461 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17521 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17581 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17641 cacctgcaca tcatattccc aggcgatttt tgtcagttcg cccagcgtat gcagctcggc
    17701 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17761 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17821 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gcgagacgat
    17881 accggtcagg cgtttcgcgg tcatcggcac atagcgcagc agcacccccg catggatagt
    17941 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18001 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18061 cggcactggg ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18121 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18181 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaaatt
    18241 gcgaccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18301 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18361 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cggatgcgct cgcggcccat
    18421 attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc gggcgtagtg
    18481 cagttgtgtg acgcggcgtc ctgctttggc gcgttttggc gttaatacgc cgctaaaacg
    18541 cagctcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18601 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18661 ctgcacgtta atggcgatct gcggatcgcc atacgggccg gaggtgtcgt agaccggaat
    18721 cgcttcgttt tcttcgtact gcggctgttc tttgctaccg ccaattagcg tcgggctaag
    18781 ctggatctca cgcatcggca cgcgcacgcc gggttgtgtg ccagtgatat aaatgcgttt
    18841 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggcct gggcgcgttg
    18901 ttcgcgacgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    18961 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19021 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19081 tcagccagtt aaggcactcc gacaagaaat tagccccaca aacggggcgt tgaatgtaaa
    19141 ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg tcaagcacca
    19201 gcagaatcag cttatcttcc agcacaaagc gcgcttccag tgcctcgccg atgtcagata
    19261 aaacctgttg aaactcaagg taattatcat gatcgatagc ggtttccagg ctggaatcgt
    19321 agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg
    19381 cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg aaatgtccgg
    19441 cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg tttagcctca
    19501 tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg agcagatgct
    19561 tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt
    19621 tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact gctcagtgag
    19681 aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga gctatggatc
    19741 gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa aaattatggt
    19801 tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg
    19861 cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag cagcggcgtc
    19921 acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt caactggccg
    19981 gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt tactgcgggc
    20041 atgaaatgta tccaagcaaa accgaatggg cgatgctgtg cagccattgc cgtgagcgtt
    20101 actacccgca aatcgccccc tgcattattg ttgctatccg tcgcgatgat tcgatcctcc
    20161 tcgcccagca tacccgccat cgtaacggtg tccatactgt acttgccgga ttcgtcgaag
    20221 tgggcgaaac cctcgagcag gcggtcgcgc gggaagtgat ggaagagagc ggaattaaag
    20281 ttaaacactt gcgttatgtg acttcccagc cgtggccgtt tcctcagtct ttaatgaccg
    20341 cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg
    20401 cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc
    20461 gtctgataga agatacggtg gcgatgtgtc gggcagagta tgagtgatga tacactgacc
    20521 gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg
    20581 cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag gcgggtcgct
    20641 atctaccgga atataaagcc acgcgcgccc aggcaggcga ttttatgtcg ctgtgcaaaa
    20701 acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg
    20761 cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg ctctattttg
    20821 aggccggaga aggtccgcgt tttacctcgc cagtcacctg caaagctgac gtcgataaac
    20881 tgccaattcc ggacccggaa gatgaactgg ggtacgtgat gaacgcggtg cgtaccattc
    20941 gtcgcgaact gaaaggccaa gtgccgctga ttggtttttc cggcagcccg tggacgcttg
    21001 cgacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt
    21061 atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc gtcactctgt
    21121 atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac acctggggcg
    21181 gtgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat aaaattgttg
    21241 atggtttact gcgtgaaaac gacggtcgcc gcgtaccggt cacgctgttt accaaaggcg
    21301 gcggacagtg gctggaagcg atggcagaaa ccggttgcga tgcgctgggc ctcgactgga
    21361 caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg cagggtaata
    21421 tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta gcgactatac
    21481 ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc attcatcagg
    21541 atgtgccgcc agaacatgct ggtgtgtttg tggaggcagt gcatcgactg tctgaacagt
    21601 atcaccgcta aggagcgatt atggatctcg cgtcattacg cgctcaacaa atcgaactgg
    21661 cttcttctgt gatccgcgag gatcgactcg ataaggatcc gccggatctg atcgccggag
    21721 ccgatgtcgg gtttgagcag ggcggagaag tgacgcgagc ggcgatggtg ctgctgaaat
    21781 acccctcgct tgagctggtg gagtataaag tggctcgcat cgccaccacc atgccttaca
    21841 ttccaggttt tctttccttc cgcgaatatc ctgcgctgct ggcagcgtgg gagatgctgt
    21901 cacaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct cgccgtcttg
    21961 gcgttgccag ccattttggt ttgctggtgg atgtgccgac cattggcgta gcgaaaaaac
    22021 gactatgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc ccactgatgg
    22081 ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac ccgttgttta
    22141 tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa cgctgcatga
    22201 aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg gaacgtccgg
    22261 cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat aatttccgta
    22321 tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc ctggaaagct
    22381 ggcagcatgt cactttcatg gcttgcttat gcgaacgcat gtaccccaat tacgccatgt
    22441 tctgccagca aaccggtttt ggtgatgggc aaatttaccg tcgtattctc gatctcatct
    22501 gggaaacgct gaccgttaaa gatgcaaaag taaatttcga cagccaactg gagaaatttg
    22561 aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg gcaatcgatg
    22621 cctgcgtggc gttaagtgaa ctggtccatt cgcgtttgag tggtgaaacg ctcgaacacg
    22681 cggtggaagt gagtaagacc tccatcacga ccgttgcgat gctggaaatg actcaggctg
    22741 gtcgcgaaat gagcgatgaa gagctcaaag aaaacccagc tgtagagcaa gaatgggaca
    22801 ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc gagctgataa
    22861 aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata atttttcagc
    22921 aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc ttccctttcg
    22981 ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc gtgtatgcag
    23041 gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata aacacattgt
    23101 aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa gcagaactgt
    23161 ccaaaaccca ggctaaagct gctctggagt ccactctggc tgcaattact gagtctctga
    23221 aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac caccgcgctg
    23281 agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca gctaacgtac
    23341 cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc gtggcagtga
    23401 acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga tcattgatgc
    23461 tggctggcgc tctgtttctc actgcctgta gccacaactc ttcacttcct ccctttaccg
    23521 ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac agcggcgata
    23581 atgtgcatct tcttgccgtg tttagcccgt ggcgcagtgg cgataccacg acgcgagagt
    23641 atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc aaaccgccgg
    23701 tgaacattcg cgcgcgtttt gacgatcgcg gcgatctgag ctttatgcaa cgtgaatccg
    23761 atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat cgtgctgatc
    23821 agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc caggggcgct
    23881 ggcatgcgat ggaacagacc gtgaccacct gcgaagggca aaccattaaa cctgatttag
    23941 attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt gatgtcagcg
    24001 tggcatggct ggaagcgccc gaaggttcgc aattactgtt agtggcaaac tctgatttct
    24061 gtcgctggca acccaacgag aaaacgttct gatttaccag tggcccatcc ccatatgacc
    24121 gccaccaccg cagccgccgt agcccatgcc cattccggct ccgcgcggaa tacccgcttc
    24181 agccatcgca atatcccgtt tcacccgtaa ctcatctaac gactgacgca aattctccat
    24241 ctctttggcg accgcgttaa ttttgctgct atccggtggg ttcgcggcta acagggcatt
    24301 gtattcataa cgcttcgtca ctagttgctg ttgcagtgcg ctgctttgag cgtaaaagtc
    24361 attatggatt ttctgccacg ctgtctgttg ttcgctggtc aaaggcgcgg cattttgctg
    24421 ccacataccg tgtccgccgt gagcaaatgc agatgtcgat cccatcgcca ttgctgaaag
    24481 cgccatcatc accagggcaa ttttcgtgtt ccgtttcatg gttaatcctc cagtggttgt
    24541 cttacttcgg gtattgcatc ttccgtgcca acgatgaaac gctgatatga cgggtaatct
    24601 ggcatgataa acgagtaaaa atgactcgcc tgctgcgggt agcgagtcat ttttactcat
    24661 tgaaacttaa gcctttgtgt tacagcgcag ggtaagcgct gataaaagat ggcatgattt
    24721 ctgctgtcag aaagggatga gcaggcaaag aagaagatgc gttttatgca acgttctaaa
    24781 gactccttag ctaaatggtt aagcgcgatc ctccccgtgg tcattgttgg gctggtaggg
    24841 ttgtttgcgg tgacggtgat tcgcgattat gggcgcgaga ctgccgctgc cagacaaacg
    24901 ctgctggaaa aaggcagtgt acttattcgt gctctcgaat ccggctcgcg cgtcggcatg
    24961 gggatgcgca tgcatcatgc gcagcagcag gccttactgg aagaaatggc cgggcagcct
    25021 ggagtacgtt ggtttgcggt cacggatgaa caaggaacaa tcgtgatgca tagcaactcc
    25081 ggcatggtgg gaaaacagct ttattccccg caggaaatgc agcagttaca tccgggagat
    25141 gaagaagtgt ggcggcggat cgatagcgca gacggtgagc ctgttctgga aatttatcgc
    25201 cagtttcaac cgatgtttgc tgctggaatg caccggatgc gccatatgca gcagtatgcc
    25261 gcgacaccac aagcaatttt catcgctttc gacgccagta atattgtgag tgccgaagat
    25321 cgtgagcaga gaaacaccct gattatcctc ttcgccctgg cgacggtctt gctggcaagc
    25381 gtattgtcat tcttctggta tcgccgctat ctgcgctcgc gccagcttct acaagatgaa
    25441 atgaagcgca aagagaagct ggtggcgctg gggcatcttg cggcaggcgt tgcccacgaa
    25501 atccgtaacc cactttcctc gattaaagga ctggcgaaat actttgccga gcgcgcgcct
    25561 gcagggggag aagcgcatca actggcgcag gtgatggcga aagaggccga ccgtttaaac
    25621 cgcgtggtaa gcgagttgct ggaactggtt aagccaacgc atctggcttt gcaggcggtg
    25681 gatctcaaca cgctgattaa ccactcatta cagctggtaa gtcaggatgc aaacagccgg
    25741 gagatccagt tacgctttac cgccaacgac acattaccgg aaattcaggc cgacccggac
    25801 aggctgactc aggtcctgtt gaatctctat ctcaatgcta ttcaggcgat tggtcagcat
    25861 ggcgtgatta gcgtgacggc cagcgaaagc ggcgcgggcg tgaaaatcag cgttaccgac
    25921 agcggtaagg gaattgcggc agatcagctt gatgccatct tcactccgta cttcaccact
    25981 aaagccgaag gcaccggact ggggctggcg gtcgtgcata atattgttga acaacacggt
    26041 ggtacaattc aggtcgcaag ccaggaggga aaaggctcaa cgttcaccct ctggcttccg
    26101 gtcaatatta cgcgtaagga cccacaagga tgacgcacga taatatcgat attctggtgg
    26161 tggatgatga cattagccac tgcactattt tgcaggcttt actgcgcggc tggggctata
    26221 acgtcgcgct ggcgaacagc gggcgacagg cgttggagca ggtgcgggaa caggtttttg
    26281 atcttgtgct ttgcgatgtg cgaatggcgg agatggacgg cattgccacg ctaaaagaga
    26341 tcaaagcgtt aaatccggcg attccggtac tgattatgac cgcgtactcc agcgtcgaga
    26401 cggcggtaga agcgttgaaa accggggcgc tggattatct catcaagccg ctggatttcg
    26461 ataacctgca ggcgacgctg gaaaaggcgc tcgcgcatac gcacagtatt gatgctgaaa
    26521 cgcctgcggt gactgccagc cagttcggta tggtcggtaa aagcccggcg atgcaacacc
    26581 tgctcagtga aatcgccctc gtcgcgccat cggaagccac ggtgctgatc cacggcgatt
    26641 ccggcaccgg taaagagctg gtcgccaggg cgattcatgc cagtagcgca cgtagcgaaa
    26701 aaccgctggt aacgctcaac tgtgcggcac tcaacgaatc cttgctggaa tctgaattgt
    26761 tcggtcacga aaaaggggcg ttcactggag ccgataaacg ccgggaaggg cgctttgttg
    26821 aggcggacgg cggcacgctg tttctcgatg aaattggcga tatctcgccg atgatgcagg
    26881 tgcgtctgct gcgtgcgatt caggagcgcg aagttcagcg tgtcggtagc aaccagacta
    26941 tctcggttga tgtccggttg attgcggcga cccatcgcga tcttgccgca ggggtaaagg
    27001 ccgggcgttt tcgccaggat ctctactatc gcctgaatgt ggtggcgatt gaagtaccat
    27061 cgctgcggca acggcgggaa gatatccctc tgctggctgg ccattttctt cagcgctttg
    27121 ccgagcgtaa tcgcaaggcg gtaaaaggct ttacgccaca ggcgatggat ttgctgatcc
    27181 actacgactg gccgggaaat attcgtgaac tggaaaacgc ggtggaacgg gcggtggtgc
    27241 tgctgaccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc ggtacgccga
    27301 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtcgaaaaa gaagtgattc
    27361 tggcggcact ggagaaaacg ggcggcaaca aaaccgaagc cgcccgtcag ttagggatca
    27421 cgcgcaaaac gctattggca aaactgtcgc gttagttctg ctcgcgttcg atagcgcgcc
    27481 agccgatatc tttccggcag aagcagtcgt cccagtgaat atcggtcatt aaggcataag
    27541 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27601 cgctggtcac tacctgctcg ttatccgcca gtttcgtgcc cgcgtggaac actttgccgt
    27661 cagccacttc ttccagcggc aggccgtgga tcacatcgcc ggtgcggtaa tcgcccggat
    27721 atccacccgc agccatcacc acgccgagag aagcacgttc atcccactct gacgttttct
    27781 cgtccagttt gccttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27841 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27901 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    27961 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacttcat
    28021 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc cgtgtttggt ccggtatctt
    28081 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28141 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28201 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28261 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28321 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28381 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28441 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcagcgcgg aaggtatcga
    28501 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28561 gtgcgaaatc caacagcgcc gggatatcgg tcacgccgat agcaacgttt tgcagcgcgg
    28621 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28681 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28741 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28801 ctcgtcggcg gcggcaatca cttcgtcatc acggatagaa ccgccaggct ggattacaca
    28861 ggtcacgccc gcagccgcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28921 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    28981 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29041 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcacg
    29101 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgatcat
    29161 gcccagatcg cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat cgaggcccgg
    29221 aacacgctcg ccccactgac cgcaggtcag aacgcgtaca ttctgtttgg cggcggtgat
    29281 tttcagggct tcttcgctgg cggacggcgc aataatcact tcaacaaact gacgagaaat
    29341 gatggcctgt gcggtttccg catccagctc gcggttaaag gcaatgatgc cgccgaatgc
    29401 ggaggtcggg tcggttttgt acgcgcgatc gtaagcgtca agaatggaat tgccgatagc
    29461 cacgccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29521 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29581 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29641 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29701 gcgagggaag cgaccggcgg cttctttgct ttcaccgtgg taagccggaa ccatgctgcc
    29761 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29821 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29881 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttgg cggcggagcg
    29941 caccatcgtt gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcaaccttc
    30001 acgggccacg gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30061 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30121 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30181 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30241 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30301 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30361 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30421 gggcatcttt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30481 ttctctttca atggtgatca caattttgac agtggttacc gtgggaaaaa tacagaaatt
    30541 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30601 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30661 taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30721 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30781 cctcgcgaca gtgagctgaa agcc
//
LOCUS       DAZFFG010000046        30515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFFG010000046 DAZFFG010000000
VERSION     DAZFFG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.046"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            210..1793
                     /gene="yegH"
                     /locus_tag="ACHJ7J_002441"
     CDS             210..1793
                     /gene="yegH"
                     /locus_tag="ACHJ7J_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5000663.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2242..4095)
                     /gene="asmA"
                     /locus_tag="ACHJ7J_002442"
     CDS             complement(2242..4095)
                     /gene="asmA"
                     /locus_tag="ACHJ7J_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO5000664.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4117..4698)
                     /gene="dcd"
                     /locus_tag="ACHJ7J_002443"
     CDS             complement(4117..4698)
                     /gene="dcd"
                     /locus_tag="ACHJ7J_002443"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO5000665.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4790..5431)
                     /gene="udk"
                     /locus_tag="ACHJ7J_002444"
     CDS             complement(4790..5431)
                     /gene="udk"
                     /locus_tag="ACHJ7J_002444"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO5000666.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5749..9066
                     /gene="dgcE"
                     /locus_tag="ACHJ7J_002445"
     CDS             5749..9066
                     /gene="dgcE"
                     /locus_tag="ACHJ7J_002445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5000667.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     ISLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLILLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSAMLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9167..10015)
                     /gene="alkA"
                     /locus_tag="ACHJ7J_002446"
     CDS             complement(9167..10015)
                     /gene="alkA"
                     /locus_tag="ACHJ7J_002446"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO5000668.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAGR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10149..11501
                     /gene="yegD"
                     /locus_tag="ACHJ7J_002447"
     CDS             10149..11501
                     /gene="yegD"
                     /locus_tag="ACHJ7J_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5000669.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11513..13453)
                     /gene="yegI"
                     /locus_tag="ACHJ7J_002448"
     CDS             complement(11513..13453)
                     /gene="yegI"
                     /locus_tag="ACHJ7J_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO5000670.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYVSDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPAVQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSAVSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTSRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRTNEAVTPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13450..14211)
                     /gene="pphC"
                     /locus_tag="ACHJ7J_002449"
     CDS             complement(13450..14211)
                     /gene="pphC"
                     /locus_tag="ACHJ7J_002449"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO5000671.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVCLNDQQPILVMFL
                     ADGAGSVSQGGEGAKLAVNEATAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14208..14867)
                     /gene="yegL"
                     /locus_tag="ACHJ7J_002450"
     CDS             complement(14208..14867)
                     /gene="yegL"
                     /locus_tag="ACHJ7J_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5000672.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15088..15147)
                     /gene="ibsA"
                     /locus_tag="ACHJ7J_002451"
     CDS             complement(15088..15147)
                     /gene="ibsA"
                     /locus_tag="ACHJ7J_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO5000673.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15420..15476)
                     /locus_tag="ACHJ7J_002452"
     CDS             complement(15420..15476)
                     /locus_tag="ACHJ7J_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5000674.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15748..15804)
                     /locus_tag="ACHJ7J_002453"
     CDS             complement(15748..15804)
                     /locus_tag="ACHJ7J_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5000675.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16083..17330
                     /gene="mdtA"
                     /locus_tag="ACHJ7J_002454"
     CDS             16083..17330
                     /gene="mdtA"
                     /locus_tag="ACHJ7J_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO5000676.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASLASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATAPEEKATSREYAKKGARS"
     gene            17330..20452
                     /gene="mdtB"
                     /locus_tag="ACHJ7J_002455"
     CDS             17330..20452
                     /gene="mdtB"
                     /locus_tag="ACHJ7J_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO5000677.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTS
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDISSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQWLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20453..23530
                     /gene="mdtC"
                     /locus_tag="ACHJ7J_002456"
     CDS             20453..23530
                     /gene="mdtC"
                     /locus_tag="ACHJ7J_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO5000678.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKSRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23531..24946
                     /gene="mdtD"
                     /locus_tag="ACHJ7J_002457"
     CDS             23531..24946
                     /gene="mdtD"
                     /locus_tag="ACHJ7J_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5000679.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNETHQNVAISRRKRSAQ"
     gene            24943..26346
                     /gene="baeS"
                     /locus_tag="ACHJ7J_002458"
     CDS             24943..26346
                     /gene="baeS"
                     /locus_tag="ACHJ7J_002458"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO5000680.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26343..27065
                     /gene="baeR"
                     /locus_tag="ACHJ7J_002459"
     CDS             26343..27065
                     /gene="baeR"
                     /locus_tag="ACHJ7J_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO5000681.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27256..27588
                     /gene="yegP"
                     /locus_tag="ACHJ7J_002460"
     CDS             27256..27588
                     /gene="yegP"
                     /locus_tag="ACHJ7J_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO5000682.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27786..28083
                     /locus_tag="ACHJ7J_002461"
                     /pseudo
     CDS             27786..28083
                     /locus_tag="ACHJ7J_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            28085..28381
                     /locus_tag="ACHJ7J_002462"
     CDS             28085..28381
                     /locus_tag="ACHJ7J_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5000683.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEVIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28484..29845
                     /gene="yegQ"
                     /locus_tag="ACHJ7J_002463"
     CDS             28484..29845
                     /gene="yegQ"
                     /locus_tag="ACHJ7J_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO5000684.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(30175..30492)
                     /locus_tag="ACHJ7J_002464"
     CDS             complement(30175..30492)
                     /locus_tag="ACHJ7J_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000685.1"
                     /translation="MNKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         7022 a         8137 c         8061 g         7295 t
ORIGIN      
        1 ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa taatgtttta
       61 ttatcaccac ttttaattca gggataatgt gaggttatta ccctcaaata atatgagata
      121 aatagtgcgg caacattgca tttttgcccc gatttatcca tgatcgaatt gtgacatttg
      181 tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg tctatctggg
      241 ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac ctggtcttta
      301 ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg gttaccgggc
      361 tgctgctggc gatgttaatg cgcctgttac tgctggcgtc aatctcctgg ctggtcaccc
      421 tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac ttaatcatgc
      481 tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa cggctggaag
      541 ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc gtggtgacgc
      601 aaattgtggt actggacgcc atcttctcac tcgactcggt gattaccgcc gtcgggatgg
      661 tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg atgttgatgg
      721 ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt ctctgcttaa
      781 gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggtttc gtcattccga
      841 aaggctacct gtacgctgct attggtttct cggtgatgat cgaggcgctc aatcagttgg
      901 ctatctttaa ccggcgacgt tttctttccg ctaaccagac gctgcgccag cggactaccg
      961 aagcggtaat gcgcctgctt agcgggcaaa aagaggatgc ggaactggac gccgaaaccg
     1021 cgtccatgct ggtggatcat ggtaaccagc agatctttaa tccgcaggaa cggcggatga
     1081 ttgagcgggt acttaatctt aaccagcgta ccgtcagcag cattatgacg tcgcgccacg
     1141 atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg ctggagcgaa
     1201 atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg ctcggtgttg
     1261 ttcacgttat cgacctgcta caacagtctc tgcgcggcga accgctcaac ctgcgggtgt
     1321 tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc ctggagcagt
     1381 tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg gtggaaggga
     1441 ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac gaagtggaag
     1501 agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg gcgaatggtc
     1561 atatgccgct ggaagatctg gtgcaatatg tgccgctgcc gctggatgaa aaacgtgaat
     1621 atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa cctggcgaag
     1681 aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga
     1741 aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg tgatggtatc
     1801 ggtcatttta cccgatgctg cgtaacgcct tataacttca gcaatgaagc gttgtctgtg
     1861 gtacatggac attacagcgg ggggggtccg gctgtaaatc gccgaatcgt ttcctgtagg
     1921 ccggggcagc ccacatggat gtgggctgag ggcgcgtttt acagggatgt tacctcgcgc
     1981 ccgtacccga tagccgaaag gaataagatg agggaaccgc gccagcggcg atttaccgcc
     2041 gggagcccgg gttgccaagg tggtggcgat tgagccaccc tggcacgttc accggcttga
     2101 gcgttccata gtagcgagga acatcaagtg aacggaacag ccttcgatct tacatattca
     2161 gaatataatg gtgccgccgc gaacgcctga tgcgacgctt tacgcgtctt atcaggccta
     2221 caggacaggt cacctggtaa cctacatctt ctccagcaac ttcttcacat ctttgccatt
     2281 gcgggaatct ttattccgct cggcccagtc attcaggcga cgtttcgctt cgtcctgtag
     2341 atgtttgcgc agtaactgat ccacttgcag actgtaattg agttgctgcc agttgccata
     2401 aacccgcagc ggtactggcg tttctttgag gaaatcaatc agtttgcttt ccccgttcca
     2461 gccacccacg acccgaatat caaactgggt gtcgcaggtt tgatctgcca gattcaacat
     2521 gccttcccct gtcagcgcca gcacaggcga ttgaccttgc atgtcgttta acgtcacgac
     2581 gccatccttc aacgtcaaat cggtggtaaa gcggtcaaga cgcgttacgt tatcgaagtt
     2641 ttcagcggcc ttcacatcac caccattacg ttctaccgct tgctgaatca tctgctggaa
     2701 gttcatccct tccatgcgcg tgtcagtcat ttcgacatgt gcctgtcctt gccagttgtg
     2761 gcggaatgcg tcggcatcta tgtcagcacc ggagaaatca ccagccagtg acatttttcc
     2821 ggtcaacgaa atcggatagt taaacgcctt cagaatggta ccaatctcaa cgttttccag
     2881 ccgtggctgg aagtttatcc gcggatttat tgatgtcgcg tccagcgtgc ccggcagtga
     2941 aacctgtcca ccgttaagct tgccctgtag ttgagtaatt tccagcagac ccgacttgtt
     3001 ggtcatttgc gtggcaacat ctgtaaaatt cattccgcgc cagcgcacgt tactggcctg
     3061 caacaaaata tcagccgtaa agccttgcag tccctgatag gccggttcat caatacgcga
     3121 agaaatgacc gggcgcggca acgttgattg gctctgcccc tgctgcgcgg caccgttttc
     3181 accattcgcg gtttcattaa gcgggatgag gttgtcaaga ttcagttgcg ggaattgcag
     3241 cctcagctgc cattccggtt tctctgtcag cgtaacctgt gcttgcccgc tcagcgtact
     3301 gtcattggcg gtcaagctga tttggttaaa tgaaaggcgt ttatgtgact cctgccactg
     3361 ggcctggaaa ctcccctgcc cgtgaatacc ttgttttggt aaatcggcac cctgcaactg
     3421 ccagttaatt tgttcaatag ccgccgttaa atcatgcgga taatcagaag catctaccgt
     3481 accgttaagg gatatcgtga gatcgcgctg atcgcgatta acccgcccgg agaactcaaa
     3541 tgagccacga tgttggggat cttgttccat ttgcaggcgg atattgcgga ttgtcacctg
     3601 ctcgtcatct tcatgctgga acaccagcac gctgtccgcc accttaagac tggatatatc
     3661 aaacgaccat ccgcgatcat ctgacagatc cggcaaggta ttgtcgcgcg gtgcaaccgg
     3721 agcgtcttca ctgcgcaccg cttccgtctg cggcgtcagt tggatcactg ccccttttag
     3781 catcacctgc ttaacgctca gttgatgact cagtagtggt aaaagcgcca cgtccaggcg
     3841 catgttgtcg gcgcgaacca gtggctggct cgcgccctgg gcggtgagag acattcgccc
     3901 ggagaggata ctaagctgcg gccagacgtg ccaacgcagt ggcccgtcga gctgcaattg
     3961 ataaccgcta cgtgcagcaa cttgcttgac catatagtcg cggaaatcat tcggattcac
     4021 cagcaacact aacgcagata acccggccac cagcacgacc aggagtatca tcagcgtcgt
     4081 cagaaatcgt ctcatggtat cctcaatggg cctgaattag tctttatcga ttcggctggc
     4141 taccgcgccc tgctggttgc gatacttcgc atcttcacgg cggttgtaag gtcgcgccgc
     4201 cgggccggaa agcggctcaa agctcagcgc accaattaac atgcccggac gcagcgccag
     4261 cggcagctta ccggagttgt agaactccag cacaatgcaa ccagaccagc ccggatcgat
     4321 acggtgcgcg gtgacgtgca ccatcagccc cagacgcgcc agtgaggaac gcccgtccag
     4381 ccagcccacc agatcggctg gcagcgtcac cgactccagc gtcaccgcca gcgccagctc
     4441 tcctgggtga agatagaacg cctcgccctc gtcgagaacg atctcatcgc tcatcacgcg
     4501 gtcaagcgca gcgctcactt catctttggg accgctcaga tcgataaacg ctgccgtgtg
     4561 accacggaag gtacgaaatt tattgcccag gcgtacatcc accgtcgcgc cgttaatacg
     4621 ctccactggc ggacgtgggt tgatcgacaa acggccttca tcaagccagg cttcaatatc
     4681 tcggtcacac agacgcatgg cattttctcc tttcgcgcat cactccctta acgccaatta
     4741 cgtcaagggc ataccaggtt atcactgaac ggtacacaat ttatcaagct tattcaaaga
     4801 actgacttat tttcgctttc aatatatcga tcgcgatgcg gtttttccca ccgcgcggca
     4861 cgataatgtc cgcatattgt ttagaaggct caatgaattg caggaacatc gggcgcacgg
     4921 ttttttgata ttgcgccatc acggaatcca ttgaacgccc acgctcgtta acgtcacgct
     4981 tgatgcggcg catcaggcag atatccagcg gggtatcaac gaaaatggag aagttaagtt
     5041 cgtcacgcaa acgtgcatcc gtcagcaaca aaatgccttc gagaatgatg accttcttcg
     5101 gctcaaccgt caccgtttct ttcatacgcg tatgttcaac atagctgtaa accggcaggt
     5161 caattgccga gccgcgtttc aacgcttgta aatgctcaag cagcaggctg tgatccatcg
     5221 cgctgggatg gtcgtagttg gtcttaacgc gttcttccat cgacagatgg ctttgatctt
     5281 tgtaatagca gtcttcggga attacgccga tgtgttcatc accgacttgc tcacgtaatt
     5341 cacgataaag ggtactggca ataagactct tgccggaagc cgatgcgcca gcgataccga
     5401 taatgacgca ctgatgagac tgatcagtca tatatttagc gacctgatta acctggatgt
     5461 taggaagggg gcgacgaagc gccaaacgcg gcaattatag ggatttcatc cgcctgatac
     5521 cagtcgaata gcgttgccgc gcgctcagag ttaattgttg acgaagaatt cccggtggca
     5581 aattacgttg atcagtttta tgtaaggtaa aaaatgttat acgcggttgc gcaaattatc
     5641 cgcctttacg tcactttatg agcaattcgc atataaaatg taaaactttt gtactagcat
     5701 aaacacagaa acgaatactg gcgacctggc cttgcggata aagcggtaat gagcaaacaa
     5761 tcacagcatg tattaattgc cctgccccac ccgctgcttc acctggtcag tttaggttta
     5821 gtctcgttta tctttaccct tttctcgctt gagctttcgc agtttggcac ccaactcgcc
     5881 ccactgtggt tcccgacgtc catcatgatg gtggcgtttt atcgccatgc cgggcgcatg
     5941 tggccgggaa ttgcgctgag ctgttcgctg ggaaatatcg ccgcatccat cctgcttttt
     6001 tccaccagct cgctgaacat gacctggacg accatcaata ttgttgaagc cgtggtcggg
     6061 gcagtgctgc tacgtaaatt gctgccgtgg tataacccct tgcaaaatct ggctgactgg
     6121 ctgcgtctgg cactcggcag cgccattgtt ccacctctgt tggggggtgt tctggttgtc
     6181 ctgctgacgc ccggagacga tcctctcagg gcatttttga tatgggtact gtcagaatcc
     6241 atcggcgctc tggcactggt gccgctggga ttgttattta aaccacacta tctgctgcgc
     6301 catcgcaacc cacggttgct ttttgagtcg ctgctcacgt tagccatcac actgacgtta
     6361 agctggcttt cgatgctgta tctgccgtgg ccttttactt tcattattgt gctgttgatg
     6421 tggagcgccg tgcgcctgcc acgaatggaa gcctttttga tcttccttac cacggtgatg
     6481 atggtgtcac tgatgatggc cgcggatccc tccttgcttg ctacgccgcg tacgtacctg
     6541 atgagccata tgccgtggct accgtttttg ctgatcctgc tgcccgccaa catcatgacc
     6601 atggtgatgt atgcctttcg tgcggaacgc aaacacattt ccgaaagcga aacccgcttt
     6661 cgaaacgcga tggaatattc cgctatcggc atggcgttag tgggcaccga gggacaatgg
     6721 ctgcaatcca acaaagcact ctgccagttt ctcggttaca gtcaggaaga gctgcgcgga
     6781 ctcacctttc agcaactgac ctggccggag gatctcaata aagatctcca acaggttgaa
     6841 aagctgataa gcggtgaaat aaacacctat tcaatggaaa aacgttacta caaccgcaat
     6901 ggcgatgttg tctgggcgtt gcttgccgtc tctctggtgc gccacacgga tggcacgccg
     6961 ctctatttta tcgctcagat tgaagacatt aacgagctaa aacgcaccga acaggtcaat
     7021 cagcaactga tggaacgcat cacgctggct aacgaagcgg gcgggattgg tatctgggag
     7081 tgggagctga agccgaatat ttttagctgg gataagcgga tgttcgagct gtatgaaatt
     7141 cctccgcata tcaaaccgaa ctggcaggtg tggtacgagt gcgtgctgcc ggaagatcgc
     7201 cagcatgccg aaaaagtgat tcgtgattcg ttgcaatcac gctcgccctt taaactggaa
     7261 tttcgcatta ccgtaaaaga cggtattcgc catatccgcg ccctcgccaa ccgggtactg
     7321 aataaagaag gcgaagtcga acgcctgctc ggcattaata tggatatgac tgaggttaaa
     7381 cagcttaacg aggcattgtt tcaggaaaaa gagcgcctgc acattacgct ggactccatc
     7441 ggcgaagccg tggtctgtat tgatatggcg atgaaaatta cctttatgaa tccagtggcg
     7501 gagaagatga gcggctggac gcaggaagaa gcgttaggtg ttccgctcct gacggtgttg
     7561 catattactt ttggcgacaa cggaccatta atggagaaca tttacagtgc cgacacctca
     7621 cgttccgcga tcgaacaaga tgtggtgttg cactgtcgga gcggcggcag ttacgacgtg
     7681 cattacagta ttacgccgtt aagtactctg gacggcagca atattggttc ggttctggtg
     7741 attcaggacg ttaccgaatc acgcaaaatg ctgcgccagc tgagctacag cgcctcccat
     7801 gatgcactaa cacatctcgc caatcgcgcc agttttgaga agcaactgcg tatcctgctg
     7861 caaacggtaa acagtacaca tcagcgacat gccctggtgt ttatcgatct tgatcgcttt
     7921 aaagcggtga atgacagcgc cgggcatgcg gcgggcgacg ctttgctgcg cgaactggcg
     7981 tcgttgatgc tgagtatgct gcgctccagt gacgtgctgg cgcggctcgg cggtgatgaa
     8041 tttggtctgc tgctgccaga ctgcaatgtt gaaagcgctc gttttatcgc tacacgtatt
     8101 atcagtgctg tgaatgacta tcactttata tgggaaggcc gggtacatcg ggtaggtgcc
     8161 agcgccggga ttaccttgat cgatgacaac aatcatcagg cggcagaagt gatgtcgcag
     8221 gctgatatcg cctgttatgc ctccaaaaat ggtggccggg gccgggtgac ggtttacgaa
     8281 ccgcagcaag ctgccgcaca tagcgaacgg gcggcgatat cgcttgatga acagtggcgg
     8341 atgattaaag agaatcagtt gatgatgctc gcccacggtg tcgcttcgcc acggatcccg
     8401 gaagcgcgta atttgtggct gattttactt aagctctgga gttgcgaagg cgagattatt
     8461 gatgaacaaa catttcgtcg tagcttcagc gatccggcgc ttagccatgc tcttgaccgc
     8521 agggtattcc acgaattttt ccagcaggcc gcaaaagcag ttgccagtaa aggcataagc
     8581 atcgccctcc ccctttccgt tgccggtttg agtagcgcca tgctggtgaa tgatctgctt
     8641 gagcagctgg aaaatagccc tctaccacca cggttattac atctgattat tccggctgaa
     8701 gcgattttag atcacgcaga aagcgtgcaa aaactgcggc tggcgggatg tcggatagtg
     8761 ctcagccagg tgggccgcga tctgcaaatc ttcaactcgc tgaaagcgaa tatggcagat
     8821 tatctgctac ttgatggtga gttatgcgcc aacgtgcagg gtaatttgat ggatgagatg
     8881 ctgattacga ttattcaggg gcacgctcag cgactcggga tgaaaaccat cgccgggcca
     8941 gtcgttttac ccttagtgat ggatacgctt tctggcatcg gcgtcgatct gatttatggt
     9001 gatgtgattg ccgatgccca accgctggat ttgctggtga atagcagtta tttcgcgatt
     9061 aactgaggga gcgtttgccc attgcctgat gcgacgctaa cgcgtcttat caggcctaca
     9121 aatcgctcat tccgcaaggc gctcgcaccg cttccggcga ccaacgtcat gcttcgtctg
     9181 gctgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag ggcttccagc
     9241 gctcggcata acggcggatt tgcgccggtg tcattcccgg aaatcgctgt ttaatcagat
     9301 aatcatccgg caggaaaaca tctttcgcct gccagccacg caaagcaaaa taattcgccg
     9361 tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc acatcgcccg
     9421 gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc agcgcctctg
     9481 cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct gccagccgct
     9541 gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca taaagctgtg
     9601 ccactttggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct aaaatcgccc
     9661 gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc cgccccgcgc
     9721 ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca aacaggcggc
     9781 tcattttcgc cagacactct gcggcaacgg gttctaaacc tgcacttaaa tttatgtgca
     9841 gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg ccaaccgcca
     9901 gactacgggc ataataatcg tcagcgaccg tttccacacc gttcaccgca cgggcggcga
     9961 gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcagggta tacatcgcat
    10021 ctccttattc atccgctttc agcataaacg ttattcagac gctgcgcttt gctttcatat
    10081 tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac cggcatgggg
    10141 attaattcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg gcggtcatgc
    10201 gtgacggtaa accgcaatta ctgaaaatgg aaaacgacag cacgctgctg ccttcaatgc
    10261 tttgcgcgcc aacgcgtgaa gcggtaagtg aatggctgta ccgccatcat gatgttccgg
    10321 cagacgacga tgaaacgcag gcgctgttac gtcgggcgat tcgttataac cgcgaagaag
    10381 atatcgatgt tacggcgaaa agcgtgcagt tcggtctttc ctcactggca cagtacattg
    10441 atgatccgga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt gccagcggct
    10501 taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg ttgcacattc
    10561 gccagcaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt ggtcgcccga
    10621 tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcaaggg attctggaac
    10681 gcgcggcgaa gcgtgccgga ttcagggacg tggtattcca gtacgagccg gtagcggctg
    10741 ggctggatta cgaagccacc ttacaggaag aaaaacgggt actggtggtg gatatcggcg
    10801 gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg cgtctcgatc
    10861 gtgaagccag cctgctgggt cacagtggtt gccgtattgg cggtaacgat ctggatatcg
    10921 cgctggcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc gaaaaaggca
    10981 tcgccctgcc gattctgccg tggtggaatg cggttgccat caacgacgta cctgcgcaga
    11041 gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc gatgcccgcg
    11101 aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc tatcgcctgg
    11161 tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc cgcgcctcac
    11221 tgccgtttat cagcgatgaa ctggctacgc tgattagcca gcaagggctg gaaagcgccc
    11281 tcagccagcc actggcgcgg attctggaac aggtgcaact ggcgctggat aacgcccagg
    11341 aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg attaaaaaag
    11401 cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt ggctccgtca
    11461 ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg gctcagcagc
    11521 ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac ggttccatca
    11581 acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg taactctgtc gcgccgacag
    11641 tcaacgtcga ttccagccga tgtcgtttgg cggtcatttt cgccattacc gcttgtctgt
    11701 ctgctggcgt taccgcttca ttcgtcctga acacaaaacg gcgttcgcaa cttgctttcc
    11761 agtcgataac cgcctgtgtc agatggtcgc caaacccttt cacttgctta acgccacgac
    11821 tggtaacatc tgctgctgtt tcaataccaa aggaccgtaa cgccgcttta cgcgcagggc
    11881 caacgccggg aatagaggca gcatcaataa aaaatccctc cagaaacttc tgcttctgcc
    11941 gttcccttgc agtgtcatga agtgctgcca gagcgcgttt ctcctcttcc ggtaacccga
    12001 gaatttcgtc cttcattttt tcgagcatcg cccgtttggc gataaaacct tccagcccgc
    12061 ccaactgttg gatctggctg actaaatggt catagtccat tttcgcgcga ttaaatgcct
    12121 cacggcgttg ctggatttct gctttataag ctttgcttgt cagactgcca ataatccaga
    12181 tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg ccgcaaagca
    12241 gcagcgatac cgctgacagt gcgatctcaa gcagaatgat gtattcgcgc cgtaacaggc
    12301 ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcaat tgcactgctg
    12361 gcggtgctac tgacgccatc accatcgccc agactttcgc cagcacaaaa ttcccgccgg
    12421 tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta tccagcgtac
    12481 accacgggca gtcagtcaaa tgagcgggat aaacatgcat tgccgaaacg gtacatttct
    12541 ttaactgttg gcgtagagag tccagtgccg ctacccacgc ttttgccgtc ggacgcgcgg
    12601 ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatcg cccggtaaca
    12661 tcgacagcgg aatagatcgt ggcggcggtt ttaaaccacg tcgctggttg tctgacacgt
    12721 aggcataacg gaaatgggca atatccgtct ccagcgcatt acccgcatca gagataagcg
    12781 gcacaccaga ataaggatgc cgcccaccaa acaagacgtg aaaaatcagc aacgcaaggc
    12841 caaaattatc gtgattcgcg gtacgttcaa aaccgacaaa tgatggcaag gtttgtagct
    12901 ctggcggcgt aaaatgcgac acgccgactt cgcataaatg caacgtgcca ttagcgttaa
    12961 tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc ataaagctgt
    13021 tctggtttac gtcacccacg acgtgcccgt gctcgtgaac cgtagcaaaa gatgaagcaa
    13081 tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaactc tggcgacgat
    13141 gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc atcataaaac
    13201 cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg acataattca
    13261 acaactgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt aaggcgggtg
    13321 gcggcgtgtg ataaatctta gcgacgctat cgacaaactc ctcgatatca taaaccgcgc
    13381 cttcgccacc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta gcagtatata
    13441 aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat ccgtacgctc
    13501 gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat cgagttgttc
    13561 ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat gcggagaatt
    13621 atccagcata tttaacgcca gtcgctgaat accatccgta aatgctgcaa ctttatgcgc
    13681 acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg taatgaaatg
    13741 cgtcatattg gcatattcac ccaccatcgg cgtaagcggt aattgcagac cgtgaccaaa
    13801 atccaccact acaccgccgt cgccaatttg cataatcaag gtgccgttag ccgatgaaat
    13861 caatcccaga aacgtgcagg caaaatcgcg aactgctaat gctttggctt ctgcctcggc
    13921 aaaaagtcgt tgacgaatgg tcagtaccat atttgtggcg aggacatcat taagccccaa
    13981 ttcaccgtcc tgtacttttt gcaccataaa cgccgtcgct tcattgacgg cgagcttcgc
    14041 accttctcct ccctgcgaaa cgctaccagc gccatcagca aggaacatca ccagtattgg
    14101 ttgctggtcg tttaggcaga caacctgcat ctggcaggca tcctggcagg gtaaatcagc
    14161 gctaatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca cactgacgtc
    14221 cagccttttg gcgcttccag tacaacttcc gttcccggcg tggagcggga aaccgaacgc
    14281 agtgaactgg ataaccagct aaacagttcg cggaattgta atccctgcag agacaaaggc
    14341 tgacggacgc tgatttgggc cagcgtcttc atatccgccc cctgaacacc gatggaaaag
    14401 aaggcaaact tcttatcttc ttccccctga aacactttgt tggcggcagc ctgccactca
    14461 tcggttggag cgccatcggt gatcaggaaa atccacgggc gataatacga aataccattt
    14521 gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat ggcggcaccc
    14581 attggtgtat cgccctgggc aaacaggatg ggcgggaaaa aattagcggc gctggtaaag
    14641 ggttgttcca catgcaccgg gccgaacgtc acaataccaa gttccactct ttttaaggcc
    14701 agcgggtcgg caagcagctc atcgcgaaag gtaaccaatc cggcgttaag ttcattaatg
    14761 ggtctaccgt tcatcgaacc agaaacatcc agcagcaaaa tgcaggggca acgtggttcc
    14821 gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag aaatcctttc
    14881 ttagagaatt attgaggtcc ggtatcatac cgattgcaat aatgaaataa gagcaaacga
    14941 agaaactttc agaattaggc ccggcggcgc agaaaatttt gctattcgcc tcgcaagaac
    15001 ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg agggttaggg
    15061 agaggtttcc ccctcctcct ggtgttctta gtaagcctgg aagctaatca ctaagagtat
    15121 caccagtatg atgacgtgct tcatcataac cctttcctta ttaaaagccc tcttctccgg
    15181 gagagggttc cccgtttcag cgtcccgctg aaatcgtcgg cttacctcct ttcgccctga
    15241 atgcagtcta tcgctaacgc gtgaacaaaa tagttccctc tgtgattatt gcgtaacgac
    15301 tcgcaaagaa tatttgctgg caggatcgca gactgcaaag cctgcagatt gacaatctta
    15361 tcgtgaaggc atactttcag gagtgagggt agagcggggt ttcccccgcc ctggtagtct
    15421 tagtaagccg ggaagcttat gactaagagc accacgatga tgatccactt catcatgacc
    15481 ctttccttat ttaaggcccc ttcctcggga ggggctttcc cgtttcagcg tcccgctgaa
    15541 atcgtcggct tacctccttt cgccctgaat tcagtctatc gctaacgcgt gaacaaaata
    15601 gtttcctctg tgattattgc gtaacgtctc gcaaagaata tttgctggca ggatcgcaga
    15661 ctgcaaagcc tgcggattga cattctgaac gtgaaggcat actttcagga gtgagggtag
    15721 agcggggttt cccccgccct ggtagtctta gtatgctggg tagctaatca ctaagagcac
    15781 cacgatgata agtatcttca tcatgaccct ttccttattt aagacccctt cctcgggagg
    15841 ggctttcccg tttcagcatc ccgctgaaat cgtcggctta cctcctttcg ccatgcatgc
    15901 agtctatcgc taacgcgtag ataaaatagt ttcctgtgtc attactggat gcatgctcgc
    15961 aaagcgcacc gtcattcaga cgattcccga tagtgtttca taattcctcc atttttctcc
    16021 cttattggct ggctacacta gtatcattcc gcgaaacgtt tcaggaagag aaactcttaa
    16081 cgatgaaagg cagttataaa tcccgttggg taatcgtaat cgtggtggtt atcgccgcca
    16141 tcgccgcatt ctggttctgg caaggccgca atgactccca gagtgcagcc ccaggggcga
    16201 cgaaacaagc gcagcaatcg ccagcgggtg gtcgccgtgg tatgcgttcc ggcccattag
    16261 ccccggttca ggcggcgacc gccgtagaac aggcagttcc gcgttacctc accgggcttg
    16321 gcactattac cgctgctaat actgttacgg tgcgtagccg cgtggacggt caactgatgg
    16381 cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca gaaattgacc
    16441 ccagccagtt caaagttgca ttagcacaag cccagggcca actggcaaaa gataaagcca
    16501 cgcttgccaa cgcccgccgt gacctggcgc gttatcaaca actggtaaaa accaatctcg
    16561 tatctcgtca ggaactggat gcccaacaag cgctggtcag tgaaaccgaa ggcaccatta
    16621 aggctgatga agcaagcctc gccagcgcac agctgcaact cgactggagc cgcatcaccg
    16681 caccagtcga tggtcgcgtt ggtctcaagc aggttgatgt cggtaaccaa atctccagtg
    16741 gtgataccac cgggattgtg gtgatcaccc agacgcatcc tatcgatttg ctctttactc
    16801 tgccggaaag cgatatcgct accgtagtgc aggcgcaaaa agccggaaaa ccgctggtgg
    16861 tagaagcctg ggatcgcacc aactcgaaga aattaagcga aggcacgctg ttaagtctgg
    16921 ataaccaaat cgatgccact accggtacga ttaaagtgaa agcacgcttt aataatcagg
    16981 atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac accgaacaaa
    17041 acgccgtggt gatccccacc gccgccctgc aaatgggcaa cgaaggccat tttgtctggg
    17101 tactgaatag cgaaaacaaa gtcagcaaac acctggtgac gcctggcatt caggacagcc
    17161 agaaagtggt gatccgcgca ggtatttctg cgggcgatcg cgtggtgaca gacggcattg
    17221 atcgcctgac cgaaggggcg aaagtggaag tggtagaagc ccagagcgcc accgcaccgg
    17281 aagagaaagc caccagccgc gaatacgcga aaaaaggagc acgctcctga tgcaggtgtt
    17341 acccccgagc agcacaggcg gcccgtcgcg cctgtttatt atgcgtcctg tggccaccac
    17401 gctgctgatg gtggcgatct tactcgccgg gattatcggt tatcgcgccc tgcccgtttc
    17461 ggcgctgccg gaagtggact atccgaccat tcaggtggtc acgctctacc caggtgcgag
    17521 cccggatgtc atgacctctg ccgttaccgc gccgctcgaa cgccagttcg ggcagatgtc
    17581 tggtctgaaa cagatgtcgt cgcaaagttc cggcggtgcg tcagttatca ctttgcagtt
    17641 ccagctaaca ttgccgctgg atgtcgccga gcaggaagtt caggccgcga ttaacgccgc
    17701 cactaacctg ctacctagcg atctgcctaa cccgccggtt tacagcaagg tgaacccggc
    17761 agatccgccg atcatgacgc tcgccgtcac ctcaaccgcc atgccgatga cgcaagtgga
    17821 agatatggtg gaaacccgcg tcgcgcagaa aatctcgcag atttccggcg tcggcctggt
    17881 gacgctttcc ggcggccagc gtccggctgt tcgcgtcaaa cttaacgctc aggcgattgc
    17941 cgccctcggc ctgaccagcg aaaccgtgcg caccgccatt accggcgcta acgttaactc
    18001 ggctaaaggt agccttgacg gcccttcccg tgcggttacg ctttccgcta acgaccagat
    18061 gcaatccgcc gaagagtatc gccagctaat catcgcctac cagaacggcg cgccgattcg
    18121 tctgggcgat gtcgcaactg tagagcaagg tgcagaaaac agctggctcg gcgcgtgggc
    18181 gaacaaagaa caggccattg tgatgaatgt tcagcgccag cccggtgcta acattatctc
    18241 caccgccgac agcatccggc agatgctgcc acagctcacc gagagtctgc cgaaatcggt
    18301 gaaggtgacg gtactttctg accgtacctc caatatccgc gcatccgtcg atgatactca
    18361 gtttgaattg atgatggcta tcgcgctggt agtaatgatt atctacctgt ttttgcgcaa
    18421 tattccggcg accatcattc ccggcgtcgc agtgccgctg tcgttaatcg gcaccttcgc
    18481 ggtcatggtg tttctcgatt tttcaatcaa taacctgacg ctgatggcgt taactatcgc
    18541 caccgggttc gtggtcgatg acgccatcgt ggtgatcgaa aatatttccc gctatatcga
    18601 aaaaggcgaa aaaccgttgg cggcggcgct caaaggcgca ggtgaaatcg gctttaccat
    18661 tatctcgctg accttctcgc tgattgcggt gttgatccca ctgctgttta tgggcgatat
    18721 cgtcgggcga ctgttccgcg aatttgctat taccctggcg gtagcgattt taatctcagc
    18781 cgtggtgtcg ctaaccctga caccgatgat gtgcgcacgg atgctcagcc aggagtcgtt
    18841 gcgtaaacag aaccgcttct cccgtgcctc ggaaaaaatg ttcgacagga taatcgccgc
    18901 ctatggtcgt ggactggcga aagtgctgaa tcatccgtgg ctgaccttaa gcgtggcgct
    18961 cagcacgctg ctgcttagcg ttctattgtg ggtgttcatt ccgaaaggtt tcttcccggt
    19021 acaggataac ggcattattc agggcacttt gcaggcaccg caatccagct cctttgccaa
    19081 tatggcccag cggcaacgcc aggtcgcgga tgtgattctg caggatccgg cggtgcaaag
    19141 cctgacctca tttgttggcg ttgatggcac taacccgtcg ctgaacagtg cgcgtttgca
    19201 gatcaacctc aaaccgctgg atgaacgtga tgatcgggtg caaaaagtca tcgcccgtct
    19261 gcaaacggcg gtggataaag tgccgggcgt cgatctcttc ctgcaaccaa cgcaggacct
    19321 gaccatcgat actcaggtca gccgcaccca gtaccagttt accttgcagg ccacgtcact
    19381 ggatgcgctc agtacctggg tgccacagtt gatggaaaaa ctccagcagc tgccacagct
    19441 ttctgatatc tccagcgact ggcaggacaa agggctggtg gcgtatgtca atgttgatcg
    19501 cgacagcgcc agccgtctgg ggatcagcat ggcggacgtc gataacgccc tgtacaacgc
    19561 gtttggtcag tggctgattt ccactattta tactcaggct aaccagtatc gcgtggtgct
    19621 ggagcacaac accgaaaata ccccagggct tgcggcgctg gataccattc gcctgaccag
    19681 cagcgacggc ggcgtggtgc cgttaagctc aattgccaaa gttgaacagc gttttgcgcc
    19741 gctttccatc aaccatctgg atcagttccc ggtaacgacc atctctttta acgtgccaga
    19801 taactattcg ctgggcgatg cggtgcaggc gattatggac accgaaaaga cgctgaatct
    19861 gccggtggat atcaccacgc agttccaggg cagcaccctc gccttccagt cggcgctggg
    19921 cagcactgtc tggctgattg tcgcggcggt ggtggcgatg tatatcgtgc tcggcattct
    19981 gtacgagagc tttattcacc cgatcaccat tctctcgacg ctacccacgg caggtgttgg
    20041 cgccctgctg gcgttaatga ttgccggtag cgaactggat gtgattgcga ttatcggcat
    20101 tattttgctg atcggtatcg tgaagaagaa cgccatcatg atgatcgact tcgcgctggc
    20161 tgctgagcgc gagcaaggca tgtcgccgcg cgatgcaatc taccaggctt gtctgttgcg
    20221 ttttcgtccg atcctgatga ccaccctggc ggctctgctt ggcgcgctgc cgctgatgtt
    20281 gagtaccggg gtcggcgcgg aactgcgtcg tccgttaggt atcggcatgg tcggcggcct
    20341 gattgtcagc caggtgctga cgctgttcac cacaccggtg atttatctgc tgtttgaccg
    20401 cctggcattg tggaccaaaa gccgctttgc ccgtcatgaa gaggaggcgt aagtgaagtt
    20461 ttttgccctc ttcatttacc gcccggtggc gacgatttta ctgtcggttg ccattaccct
    20521 gtgcggcata ctgggtttcc gtatgctgcc ggtcgccccg ctgccgcagg tcgattttcc
    20581 ggtgattatg gtcagcgcct cgctgcccgg tgcatcgccg gagacaatgg cgtcttccgt
    20641 tgccacgccg ctggagcgct cacttgggcg cattgccggg gtcagtgaaa tgacctccag
    20701 tagttcgctc ggcagcacgc gcattatttt gcagtttgat tttgaccggg atatcaacgg
    20761 cgcagcgcgt gatgtgcagg cggcgatcaa cgctgcacaa agtttgctgc ccagtgggat
    20821 gcccagccgt ccgacctatc gcaaagcgaa cccgtcggat gcgccaatta tgatcctgac
    20881 gctaacctcc gatacttatt cgcagggtga attgtacgat ttcgcctcga cgcagctggc
    20941 tccgacgatt tcgcaaatcg acggcgtcgg tgatgtcgat gtcggcggca gctcgctgcc
    21001 cgccgtgcgc gtcgggctga acccgcaggc gctgtttaat cagggcgtgt cgctggacga
    21061 cgtacgcact gccatcagca atgccaacgt gcgtaaaccg cagggggcgc tggaagatgg
    21121 cactcaccgc tggcagatcc agaccaacga tgaactgaaa accgccgctg aatatcagcc
    21181 gctgattatt cactacaaca acggcggcgc ggttcgtctg ggcgatgtgg cgacggtgac
    21241 cgactcagtg caggatgtgc gcaacgccgg gatgaccaac gccaaaccgg ctattttgct
    21301 gatgatccgc aagctgccgg aagccaatat tatccagacg gttgacagca tccgggcaaa
    21361 attaccggag ttgcaggaaa ccattccggc ggcgattgat ctgcaaattg ctcaggatcg
    21421 ctcccccacc attcgcgcct cgctggaaga agtcgagcaa acgctgatta tctcggtggc
    21481 gctggtgatt ctggtggtct ttttattcct gcgctcgggt cgcgccacta ttattcccgc
    21541 cgttgcagtg ccggtttcgc tgattggtac gtttgcggcg atgtacctgt gcggattcag
    21601 tctcaataac ctgtcgttaa tggcgctcac catcgccaca ggctttgtgg tggatgacgc
    21661 catcgtggtg ctggaaaaca ttgcccgtca tctggaagcg ggaatgaaac cactacaggc
    21721 cgcgctgcaa ggcacccgtg aagtcggctt tacggtgctg tcgatgagtc tgtcactggt
    21781 ggcggtgttc ctgccgctgc tgttgatggg cggattgccg ggtcgcctgt tacgcgaatt
    21841 tgccgtgacg ctttctgtcg ccattgggat ttcgctgctg gtttccctga cgctaacgcc
    21901 aatgatgtgt ggctggatgc tgaaagccag caagccgcgt gagcaaaagc gactgcgtgg
    21961 ttttggtcgc atgttggtag ccctgcaaca aggctatggt aagtcgctga aatgggtgct
    22021 caatcatacc cgtctggtgg gcgtggtgct gcttggcacc attgcactga atatctggct
    22081 gtatatctcg atcccgaaaa ccttcttccc ggagcaggac accggcgtgt tgatgggcgg
    22141 gatacaggca gatcagagta tttcgtttca ggcaatgcgc ggcaaattgc aggatttcat
    22201 gaaaattatc cgtgacgatc cggcagtgga taatgtcacc ggctttaccg gcggttcacg
    22261 agttaacagt gggatgatgt ttatcactct gaagtcacgc gacgaacgca gcgaaacggc
    22321 gcagcaaatt atcgaccgtt tgcgcgtgaa gctggcaaaa gaaccggggg cgaatctgtt
    22381 cctgatggcg gtacaggata ttcgcgttgg cggacgtcag tcgaacgcca gctaccagta
    22441 cacgttgtta tccgacgacc tggcggcact gcgcgaatgg gagccgaaaa tccgcaaaaa
    22501 actggcgaca ttgccggaac tggcggacgt gaactccgat cagcaggata acggcgcgga
    22561 gatgaatctg gtttacgatc gcgacacgat ggcacggctg ggaatcgacg tgcaggccgc
    22621 caacagcctg ttaaataacg ccttcggtca gcggcaaatc tcgaccattt accagccgat
    22681 gaaccagtat aaagtggtga tggaagtgga tccgcgctat acccaggaca tcagtgcact
    22741 ggaaaaaatg ttcgttatca ataacgaagg caaagcgatc ccgctgtcgt atttcgccaa
    22801 atggcaaccg gcgaatgctc cgctgtcagt gaatcatcag ggattatcgg cggcctcgac
    22861 catttcgttc aacctgccga ccgggaaatc gctctcggac gccagtgcgg caatcgaccg
    22921 cgcaatgacc cagcttggtg taccttctac cgtgcgcggc agttttgccg gaacggcgca
    22981 ggtgttccag gagacgatga actcacaggt gattctgatt atcgcagcca tcgccacggt
    23041 ctatatcgtg ctgggtatcc tttacgagag ttacgtacat ccgctgacga ttctctccac
    23101 cctgccctcg gcgggcgttg gagcgctgct ggcgctggag ctgttcaatg ccccgttcag
    23161 cctaatcgcc ctgataggga tcatgctatt aatcggcatc gtgaagaaaa acgccattat
    23221 gatggtcgat tttgcgcttg aagcccaacg gcacggtaac ctgacgccgc aggaagctat
    23281 tttccaggcc tgtctgctgc gttttcgccc gattatgatg actaccctgg cggcgctgtt
    23341 tggcgcgctg ccgctggtgt tgtcgggcgg tgacggctcg gagctgcggc aacccttggg
    23401 gatcaccatt gtcggcggtc tggtgatgag tcagctcctg acgctgtata ccacgccggt
    23461 ggtatatctc tttttcgacc gtctgcggct gcgtttttcg cgtaaaccta aacaaacggt
    23521 aaccgagtaa atgacagatc ttcccgacag cacccgctgg caattgtgga ttgtggcttt
    23581 cggcttcttt atgcagtcgc tggacaccac catcgtaaac accgcccttc cctcaatggc
    23641 gcaaagcctc ggggaaagtc cgttgcatat gcacatggtc attgtctctt atgtactgac
    23701 cgtggcggtg atgctgcccg ccagcggctg gctggcggac aaagtcggcg tgcgcaatat
    23761 tttctttacc gccatcgtgc tgtttactct cggttcattg ttttgcgcgc tttccggcac
    23821 gctgaacgaa ctgttgctgg cacgcgcgtt acagggcgtt ggcggcgcga tgatggtgcc
    23881 ggtcggcaga ttgacggtga tgaaaatcgt accgcgcgag caatatatgg cggcgatgac
    23941 ctttgtcacg ttacccggtc aggtcggtcc gctgctcggt ccggcgctcg gcggtctgct
    24001 ggtggagtac gcgtcgtggc actggatctt tttgatcaac attccggtgg ggattatcgg
    24061 tgcgatcgcc acattgatgt taatgccgaa ctacaccatg cagacgcggc gctttgatct
    24121 ttccggcttt ttattgctgg cggttggcat ggcggtatta accctggcgc tggacggcag
    24181 taaaggtaca ggtttatcgc cgctggcgat tactggcctg gtcgcagttg gtgtggtggc
    24241 actggtgctt tatctgctgc acgccagaaa taacaaccgc gctcttttca gtctgaaact
    24301 gttccgcact cgtacctttt cgctgggcct ggcggggagc tttgccggac gtatcggtag
    24361 cggcatgttg ccctttatga cgccggtttt cctgcaaatt ggccttggct tctcaccgtt
    24421 tcatgccgga ctgatgatga tcccgatggt gcttggcagc atgggaatga agcgaattgt
    24481 ggtacaggtg gtgaatcgct ttggttatcg tcgggtactg gtagcgacca cgctgggtct
    24541 gtcgctggtc accctgttgt ttatgactac cgctctgttg ggctggtact acgttttgcc
    24601 gttcgtcctg tttttacaag ggatggtcaa ctcgacccgt ttctcctcca tgaacaccct
    24661 gacgctgaaa gatctcccgg acaatctggc gagcagcggc aacagtctgc tgtcgatgat
    24721 tatgcaattg tcgatgagta tcggcgtcac catcgccggg ctgttgctgg gactttttgg
    24781 ttcgcagcat gtcagcgtcg acagcggcac cacacaaacc gtctttatgt acacctggct
    24841 tagcatggcg tttatcatcg cccttccggc gttcatcttt gccagagtgc cgaacgaaac
    24901 gcatcaaaat gtagctattt cgcggcgaaa aaggagcgcg caatgaagtt ctggcgaccc
    24961 ggtattaccg gcaaactgtt tctggcgatt ttcgccacct gcattgtctt gctgatcagt
    25021 atgcactggg cggtgcgtat tagttttgag cgtggcttta ttgattacat caagcatggt
    25081 aatgaacagc gattacaact gttaagtgat gcgcttggcg agcagtatgc gcagcatggc
    25141 aactggcgct tcctgcgcaa caatgatcgc tttgtctttc agatcctgcg ttcatttgaa
    25201 cacgataatt cggaagataa acccggcccg ggtatgccac cacacggctg gcgtactcag
    25261 ttctgggtgg ttgatcaaaa caacaaggtg ctggttggtc cgcgagcgcc gattccacct
    25321 gacggtacac ggcgacccat tctggtcaac ggtgcggaag tgggcgcggt gatcgcctcc
    25381 cccgttgagc ggttaacgcg caatactgat atcaatttcg ataaacaaca acggcaaacc
    25441 agctggctga ttgtcgccct ggcaacgtta ctcgcggcgc tcgccacctt tctgctggcg
    25501 cgtggtcttt tggccccggt caaacggctg gtagacggca cgcacaaact ggcggcgggc
    25561 gatttcacta cccgcgtaac gcccaccagt gaagatgaac tgggcaaact ggcgcaagac
    25621 ttcaaccagc tcgccagcac gctggagaaa aaccagcaaa tgcggcgcga ttttatggcc
    25681 gatatttctc acgaactgcg tacgccatta gcggtgctgc gcggtgaact ggaagccatt
    25741 caggatggcg tgcgtaaatt cacgccggag acggtggcgt ctttacaggc ggaggtcggt
    25801 acactgacca aactggttga cgatcttcat cagttgtcga tgtctgatga aggcgctctc
    25861 gcctatcaaa aagcaccggt agatttgatc ccactgctgg aagtggcggg cggcgcattt
    25921 cgcgaacgat tcgccagccg tggcctgaaa ctgcaatttt ccctgccaga cagtattacc
    25981 gtatttggcg atcgcgaccg tttaatgcag ttgttcaata atttactgga aaacagcctg
    26041 cgctacactg acagcggcgg cagcctgaaa atctctgccg agcagcacga caaaacggtg
    26101 cgcctgacct ttgccgacag tgcgccaggt gtcagtgacg atcagctaca aaaattgttt
    26161 gaacgttttt atcgcaccga aggttcccgc aaccgtgcca gcggcggttc cgggctgggg
    26221 ctggcgattt gcctgaacat tgttgaagca cataatggcc gcattattgc tgcccattcg
    26281 ccttttggcg gggtaagcat tacagtagag ttaccgctgg aacgggattt acagagagaa
    26341 gtatgaccga gttaccaatc gacgaaaaca caccgcgtat tttgatcgtg gaagatgaac
    26401 cgaagctggg gcagttgctc attgattatc tgcgtgctgc gagctatgcg ccgacgctta
    26461 tcagccacgg cgatcaggta ctgccgtatg tgcgccagac accaccggat ctgatcctgt
    26521 tggatctgat gctccctggt accgatggct tgacgctgtg ccgggaaatt cgtcgttttt
    26581 ctgacattcc tatagtgatg gtgacggcaa aaatcgaaga gatcgaccgc ctgctcggac
    26641 tggagattgg cgcagatgat tatatctgta agccgtacag cccacgggaa gtggtagcgc
    26701 gcgtcaaaac cattttgcgc cgttgcaaac cgcagcgcga gttgcagcaa caggatgctg
    26761 aaagcccgtt gattatcgac gaaggtcgtt ttcaggcttc atggcgcggt aaaatgcttg
    26821 acctgacgcc tgcggaattt cgtctgctga aaacgctctc tcatgaacca ggaaaagtgt
    26881 tctcccgcga gcaattgctc aatcatcttt atgacgacta ccgcgtagta accgaccgca
    26941 ccatcgacag ccacattaaa aacctgcgcc gcaagctgga atctctcgac gccgaacagt
    27001 catttatccg cgccgtttac ggcgttggtt accgctggga agccgacgcc tgccgcatcg
    27061 tttagtttta gcgacatgac tttgttagcc ggagatggcg ttcagaccag atccggcaac
    27121 attatcccac gcatggtcag caaactgaca ttatctctcc gccctggcag gcatcagaaa
    27181 atcgctcata ctttaatcgg taaacagcac ctttagatgc tgttttcgat acgcaatttc
    27241 aatcaaggag tcattatggc tggttggttt gaactcagta agagcagtga taatcagttc
    27301 cggtttgtgc taaaagcggg caatggtgag acgatcctca ccagcgagct gtatacctca
    27361 aaagcctctg cggaaaaggg catcgcgtcg gtgcgtagca acagcccgca agaagaacgc
    27421 tatgagaaaa aaacggcaag caacggcaaa ttctatttca atctgaaagc cgccaatcat
    27481 caaattatcg gctccagcca gatgtacgcc accgcgcaat ctcgtgaaac cggaattgcc
    27541 tccgttaaag ccaatggcac aagccagacg gtgaaagaca atacgtaatt acgatgccgg
    27601 gcgtgggttg ttggcgtccg gctttatttc caaataacgc ttatttattt acctgcgcca
    27661 ttaaaatagt ggacactgat gaatactcat tgtgctttct ttgtttaaca tctccatgct
    27721 tatcctcctg aacatgttca tctgccaaaa tatcttctaa tacttttcgt gacgctatcc
    27781 ggtttatgac atacactgat gtaagatttt tacttacagg agagcataaa catggccaga
    27841 acgatgacgg ttgatctcgg cgatgagttg cgtgaattta tcgagtcctt aattgaatcc
    27901 ggcgactatc gcacccaaag cgaagtgatc cgtgagtcac tgcgcttact tcgagaaaag
    27961 caagcagagt ctcgcctgca agcattacgt gacatgcttg cagaagggtt gagtagtggt
    28021 gaagctcagc cgtgggaaaa ggacgcgttt ttacggaagg tgaaagcagg gataaggaaa
    28081 taatgtgcgt attattaaac tcatgccaaa agccaatgag gatttagaag gcatctggta
    28141 ttacagttat caccatttcg gcgaaccaca agccgacaga tatgtggaac atctttcaga
    28201 cgttctccag attctgagta acaataatat aggtacgccc agacctgaat taggcgaagt
    28261 tatttttgtt cttccttttg aacgccacgt aatttatttc ctgcaatcac caggggaaat
    28321 tatcgtcatt cgtatcctta accaaaacca ggacgccacc agacatttac actggagcta
    28381 aacccgccga ttgcgctaca atgcccgccc ttaaagtggg ggcactcccc taaccgcttc
    28441 atcaggtgaa gcggatctga cctgtcatca gaacgagaga attatgttta aaccggaact
    28501 cctttccccg gcgggaacgc tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc
    28561 tgtttatgcg ggccagccgc gttactccct gcgtgtgcgc aacaacgaat tcaaccacga
    28621 aaaccttcag ctcggcatca atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt
    28681 caacattgca ccgcacaacg ccaagctgaa aacctttatc cgtgacctga aaccggtggt
    28741 ggaaatgggg ccggatgcgc tgattatgtc cgatccaggg ctgattatgc tggtgcgtga
    28801 gcacttccct gaaatgccga tccatctctc ggtacaggct aacgccgtga actgggcgac
    28861 ggtgaaattc tggcagcaaa tgggcctgac ccgcgtgatc ctctctcgcg agctgtcgct
    28921 ggaagagatt gaagagatcc gcaatcaggt gccggatatg gagatcgaga tcttcgttca
    28981 cggcgcactg tgcatggcct actccggtcg ctgcctgctc tctggctata tcaacaagcg
    29041 cgatccgaac cagggtacct gcaccaacgc ctgccgctgg gagtacaacg tccaggaagg
    29101 gaaagaagat gacgttggca acatcgtaca caagtacgag ccgattccgg tgcaaaatgt
    29161 tgagccgacg ctgggtatcg gcgcgccaac cgacaaagtg tttatgatcg aagaagccca
    29221 gcgtccgggc gagtatatga ccgcgtttga agatgagcac ggcacttaca tcatgaactc
    29281 gaaagatctg cgcgctatcg cccatgttga acgcctgacc aaaatgggcg tgcattcgct
    29341 gaaaatcgaa ggccgtacta aatctttcta ctattgcgca cgcaccgcac aggtttatcg
    29401 taaagctatc gatgatgccg ctgcgggcaa accgttcgat accagcctgc tggaaactct
    29461 ggaaggtctg gcgcatcgtg gctataccga aggtttcctg cgtcgtcata ctcacgacga
    29521 ttatcagaac tacgaatacg gttattcagt ttctgaccgc cagcagtttg ttggtgagtt
    29581 taccggtgag cgcaaggggg acctcgcggc ggtagcggtg aaaaataaat tctccgttgg
    29641 cgacagcctt gagctgatga cgccgcaagg caacattaac tttacccttg agcacatgga
    29701 aaacgccaaa ggcgaagcta tgccggtagc accaggcgat ggttatactg tgtggctccc
    29761 ggtgccgcag gatcttgagc taaattacgc gctgctgatg cgtaatttct ccggggaaac
    29821 cacgcgtaac ccccacggta agtgattaat ttcgattatt tttcccggat ggaaaattct
    29881 tagaaaccga tcacatacag ctgcatttat taaggttatc atccgtttcg ctgaaaaaca
    29941 taacccataa aatgctagct gtaccaggaa ccacctcctt agcctgtgta atctccctta
    30001 cacgggctta ttttttatgc ataagcccta tctctggtaa ccatcttcca ttgaccacat
    30061 cgatagaatt tcccttcata gcacgatgcc tttcacttat tggcatcgtg ctcgcacagg
    30121 ttccggctac gcacagccag aacgcgcatg tttgacgctt accaaaaaat attctcactc
    30181 tccacatttg aatgtcagac gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc
    30241 aactatctgc atttttttgc cctgagtaac acagaaatgg gctgcatcat tttttactat
    30301 attttctgca ccagatattc tgcccctggc taaagaagct tcggcttcgg tgtagtattg
    30361 gttatcgagt ttacgctgaa tattactttt atatgcaagg ccaaatttac cgatacttgt
    30421 ctcatcatta tgcacagcac aaccagacat aagaaaaaca ctaattaatg atatagcagc
    30481 tatcttgttc acctcacctt cccccattaa atacc
//
LOCUS       DAZFFG010000047        30206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFFG010000047 DAZFFG010000000
VERSION     DAZFFG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.047"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            132..3884
                     /gene="yfaL"
                     /locus_tag="ACHJ7J_002465"
     CDS             132..3884
                     /gene="yfaL"
                     /locus_tag="ACHJ7J_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO5000686.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMINNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSDSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNEGSFAGTIEGAGQLTIAQNGSY
                     VLAGASSMALTGDIVVDDGAVLSLEGDAADLASLQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVRLAND
                     NQYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDNQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISNGTVLR
                     LTGQDTSVDLDASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     TGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSASDQLVLNGNTAGNTTVVIN
                     PITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVMPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMMVKW"
     gene            complement(4025..4747)
                     /gene="ubiG"
                     /locus_tag="ACHJ7J_002466"
     CDS             complement(4025..4747)
                     /gene="ubiG"
                     /locus_tag="ACHJ7J_002466"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO5000687.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4894..7521
                     /gene="gyrA"
                     /locus_tag="ACHJ7J_002467"
     CDS             4894..7521
                     /gene="gyrA"
                     /locus_tag="ACHJ7J_002467"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO5000688.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7670..9358
                     /gene="yfaA"
                     /locus_tag="ACHJ7J_002468"
     CDS             7670..9358
                     /gene="yfaA"
                     /locus_tag="ACHJ7J_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO5000689.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNNETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DEEQDTEATAIASDLLSGKKRWQASFGLEERTAGKTPVRQRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9355..9978
                     /gene="yfaT"
                     /locus_tag="ACHJ7J_002469"
     CDS             9355..9978
                     /gene="yfaT"
                     /locus_tag="ACHJ7J_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO5000690.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGNAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10002..14516
                     /locus_tag="ACHJ7J_002470"
     CDS             10002..14516
                     /locus_tag="ACHJ7J_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO5000691.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVAQVLPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLDGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEALRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWPGMQVK"
     gene            14517..16166
                     /gene="yfaQ"
                     /locus_tag="ACHJ7J_002471"
     CDS             14517..16166
                     /gene="yfaQ"
                     /locus_tag="ACHJ7J_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO5000692.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFANGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGLGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16171..16947
                     /gene="yfaP"
                     /locus_tag="ACHJ7J_002472"
     CDS             16171..16947
                     /gene="yfaP"
                     /locus_tag="ACHJ7J_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO5000693.1"
                     /translation="MRKIFLALLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17222..20071
                     /gene="rcsC"
                     /locus_tag="ACHJ7J_002473"
     CDS             17222..20071
                     /gene="rcsC"
                     /locus_tag="ACHJ7J_002473"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO5000694.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKALGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20271..20921)
                     /gene="rcsB"
                     /locus_tag="ACHJ7J_002474"
     CDS             complement(20271..20921)
                     /gene="rcsB"
                     /locus_tag="ACHJ7J_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO5000695.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20938..23610)
                     /gene="rcsD"
                     /locus_tag="ACHJ7J_002475"
     CDS             complement(20938..23610)
                     /gene="rcsD"
                     /locus_tag="ACHJ7J_002475"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO5000696.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            24349..25476
                     /gene="ompC"
                     /locus_tag="ACHJ7J_002476"
     CDS             24349..25476
                     /gene="ompC"
                     /locus_tag="ACHJ7J_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5000697.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDIGSIDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGQNGSVSGENDPDFTGHGITNNGRKALRQNGDGVGGSITYDYEGFGI
                     GGAISSSKRTWDQNNTGLIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            25588..26643
                     /gene="apbE"
                     /locus_tag="ACHJ7J_002477"
     CDS             25588..26643
                     /gene="apbE"
                     /locus_tag="ACHJ7J_002477"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO5000698.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            26717..27781
                     /gene="ada"
                     /locus_tag="ACHJ7J_002478"
     CDS             26717..27781
                     /gene="ada"
                     /locus_tag="ACHJ7J_002478"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO5000699.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGASRKAQLLRREAENEER"
     gene            27781..28431
                     /gene="alkB"
                     /locus_tag="ACHJ7J_002479"
     CDS             27781..28431
                     /gene="alkB"
                     /locus_tag="ACHJ7J_002479"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO5000700.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            28507..30150
                     /gene="yojI"
                     /locus_tag="ACHJ7J_002480"
     CDS             28507..30150
                     /gene="yojI"
                     /locus_tag="ACHJ7J_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO5000701.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         7325 a         7406 c         8215 g         7260 t
ORIGIN      
        1 actgatacta ttcatcaaat aataacaaaa tagccattgc atcgggttgg acaatttaca
       61 aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata caaaatgatg
      121 gtcagattaa tatgcggatt atctttctac gcaaggagta tttatctcta ctcccgtcaa
      181 tgattgcatc tcttttctct gctaacggtg tcgcggcggc cattgattta tgccagggat
      241 atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt acgcaggtct
      301 ggagtatcgc cgatgggcaa tggctggttt tttcggatat gatcaataat gccagcggtg
      361 gggccgtatt tttgcaacaa ggagcggaat ttacattatc accagaaaat gaaactggaa
      421 tgactctgtt tgccaataac accgtttcag gagaatataa taacggcggg gcaatatttg
      481 ctaaagaaaa ctcaacgctg aatcttacgg atgttatttt ttctggtaac gtcgcaggcg
      541 gctatggtgg cgcaatctat tcttctggta ctaacgatac cggtgccatc gatttacgtg
      601 tcactaacgc cgtgtttcgc aataacatcg ctaatgacgg caaaggtggt gcaatttata
      661 ccatcaataa tgatatctat ttaagtgatg atgtttttaa caataaccag gcatatacat
      721 caacaagtga cagtgatggc gatggcggcg caatcgatgt cacagataat aatagcgaca
      781 gcaagcatcc ttcaggttat acgataataa ataacactgc ctttacaaat aacactgccg
      841 aaggttatgg cggggcgata tataccaata gcgcgacggc tccctatctt attgatattt
      901 ctgttgatga tagctacagc cagaacggag gcgtgttagt cgatgagaac aatagcgcag
      961 caggctatgg agatggtcct tcctctgcgg cgggtggctt tatgtatctc ggcttaagtg
     1021 aagttacctt tgatattgcc gacggaaaaa cgctggttat cggcaataca gagaatgacg
     1081 gagctgttga ctctattgct ggtaccgggt tgatcaccaa aacaggttcc ggcgatctgg
     1141 tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac ggtgaagtta
     1201 ccctgggccg cagcaactcc ctgatgaatg tcggcgatac gcattgccag gacgatccgc
     1261 aagactgcta cggtctgacg atagggagta ttgataagta ccagaatcag gcagagctaa
     1321 atgttggctc cacccaacaa acctttgtgc actcattgac gggctttcag aatggcactt
     1381 taaatatcga tgctggtggc aatgttactg ttaatgaagg cagttttgct ggcaccatcg
     1441 aaggtgctgg tcagctcacc attgcgcaaa acggcagcta tgtgctggcg ggagcgtcgt
     1501 cgatggcgct aaccggcgat atagtcgttg atgatggtgc ggtgctttcg ctggaaggcg
     1561 acgcggcaga tcttgcctct ctccaggacg atccgcagtc gatcgtgtta aacggcggtg
     1621 tgctcgatct ctctgatttc tccacctggc agagcggtac atcatacaaa gatggccttg
     1681 aagtcagcgg cagcagcgga acggttatcg gcagtcagga tgtggtagat cttgcaggcg
     1741 gaaacgatat gcatatcggc ggtgacggga aagatggcgt ctacgtggtg atcgatgcgg
     1801 gtgacgggca ggtccgcctg gcaaatgaca atcaatacct cggcacaacg caaatcgcct
     1861 ccggtacgct gatggtgagc gacaactcgc agcttggaga tacccattac aaccgccagg
     1921 ttatctttac cgataatcaa caagaaagcg tgatggagat tactgccaat gtcgatactc
     1981 gctctacaac gactgagcat gggcgtgata ttgaaatgcg cgccgacggt gaagtggcag
     2041 ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc gggcagcatc
     2101 aggatgaggg tagcacattg acgaaaacgg gggcgggtac actggagctg accgccagcg
     2161 gtacaacgca gtcagcggtg agagtagaag agggcacgct gcaaggtgat gttgcggata
     2221 tcttccctta tgcttcgtcg ctatgggtcg gtgacggggc aacgttcgtt actggcgcgg
     2281 atcaggatat tcagtcaatt gatgctactt ccagcggcac tatcgacatc agcaatggta
     2341 cggttttgcg cctgaccggg caggatactt ccgtcgacct tgatgcctca ctgtttaacg
     2401 gcgatggtac gctggtgaat gccaccgatg gcgtgacgtt gacaggtgag cttaatacca
     2461 accttgaaac tgacagcctg acttatcttt cagacgtaac agttaatggc aatctgacca
     2521 atacgaccgg tgcggtcagc ctgcaaaatg gcgtcgctgg cgatacgctg acggtaaacg
     2581 gtgattacac cggcggcggt acgttactgt tcgacagcga attaaacggc gatgactcag
     2641 caagcgacca gttggtgttg aacggtaata ctgctggcaa taccacggtt gtgattaatc
     2701 ccattacagg gattggtgag ccgacatcta caggcattaa agtggttgat ttcgcagctg
     2761 atcccacgca gtttcaaaac aatgcgcagt tcagtctggc aggcagtggc tacgtcaaca
     2821 tgggagcgta tgactacacg ctggtggaag ataacaacga ctggtatctg cgatcacaag
     2881 aagtaacgcc gccatcgcca cctgatccag acccgactcc cgatcctgat cccacgccgg
     2941 atcctgatcc aacacccgac ccggaaccta cgcctgctta ccagccggtg ttgaatgcca
     3001 aagttggcgg ttatctcaat aacctgcggg cggcaaatca ggcgtttatg atggagcgac
     3061 gcgatcacgc aggtggcgat ggtcagacgc tgaatttacg tgttatcggc ggagattatc
     3121 attacacagc agcggggcaa ctggctcaac atgaagacac ttctacggtg caacttagcg
     3181 gcgatctgtt tagcgggcgc tggggcacgg atggcgagtg gatgcttggg attgttggtg
     3241 gctacagcga taaccagggc gacagccgct cgagtatgac cggaactcgc gccgataacc
     3301 agaaccacgg ttatgcggtt gggctgacct caagctggtt tcagcacggt aagcagaagc
     3361 agggggcctg gctggataac tggttgcagt acgcgtggtt tagcaatgat gtttctgaac
     3421 atgaagatgg cgtggatcat taccattcgt cggggattat cgcctcgctg gaagcggggt
     3481 atcagtggtt accggggcgt ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg
     3541 gcgtgcagca ggatgatttt accgccgcta accgtgcgcg cgtgtcacaa tcgcagggtg
     3601 atgatattca gacgcggctg ggtttacaca gcgaatggcg taccgctgtt catgtcatgc
     3661 caacattaga tctgaattat tatcacgatc cccattcgac ggaaattgaa gaggatggca
     3721 gcactatcag tgacgatgcg gtgaagcaac ggggtgaaat aaaagtggga gtcacgggca
     3781 atatcagtca gcgagtgtcc ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt
     3841 ttgcccagac ggcagggttt ttgtcgatga tggtgaaatg gtaatcgctg tctgaccatc
     3901 actattgccg gatgcggcgt aaacgcctta tctggcctac ggagagcatt tgtaggcctg
     3961 ataagacgcg gcaagcgtcg catcaggcat tggcgcggtt gccggatgaa tccaaacaac
     4021 aacctcactt attctgcgtg tgcagcatat agttcacatc cacgccgggg ccgagtttaa
     4081 aagtattagt gatcgggttg taatgtagcc cggttatatg tcgctcttta aggcttgtct
     4141 gatctaccca gccaagcagc tctgccggtt taataaactt cttcacatca tgcgtgcctt
     4201 tgggcaccat gcgcaaaata tattccgcgc caaccaccgc catcagccat gacttgccgt
     4261 tgcggttaag cgtcgagaaa aagacatcgc cgcccggttt caccagttgc gcacaggctc
     4321 tcaccactga ctgcggatcg gggacgtgct ccagcatctc catgcaggtc accacatcat
     4381 actgcccggc atgttttgcc gcgtgctctt ccacggtttc ctgcacgtaa tccacctgaa
     4441 tgccgctttc cagcgcgtgc agctttgcca cctgcaatgg ctcaaagccc atatccagac
     4501 cggtcaccgt cgcgccttcg cgcgccatac tctcggccag aatgccgccg ccacaaccga
     4561 catcgagcac ctttttgcca aataaaccgc cagcacgctc ggcaatatag cccagacgca
     4621 gcgggttaat gcggtgcagc ggtttgaact cgccttcaag atcccaccag cgggaggcga
     4681 cggcttcaaa tttagcgatc tcttcgtggt ctacgttatg gtttaccggc gatttttcgg
     4741 cattcattgg cacttctact ccgtaattgg caagacaaac gagtatatca ggcattggat
     4801 gtgaataaag cgtataggtt tacctcaatc tgcgcggctg tgttataatt tgcgaccttt
     4861 gaatccggga tacagtagag ggatagcggt tagatgagcg accttgcgag agaaattaca
     4921 ccggtcaaca ttgaggaaga gctgaagagc tcctatctgg attatgcgat gtcggtcatt
     4981 gttggccgtg cgctgccaga tgtccgagat ggcctgaagc cggtacaccg tcgcgtactt
     5041 tacgccatga acgtactagg caatgactgg aacaaagcct ataaaaaatc tgcccgtgtc
     5101 gttggtgacg taatcggtaa ataccatccc catggtgact cggcggttta tgacacgatc
     5161 gtccgtatgg cgcagccatt ctcgctgcgt tacatgctgg tagacggtca gggtaacttc
     5221 ggttccatcg acggcgactc tgcggcggca atgcgttata cggaaatccg tctggcgaaa
     5281 attgcccatg aactgatggc cgatctcgaa aaagagacgg tcgatttcgt tgataactat
     5341 gacggcacgg aaaaaattcc cgacgtcatg ccaaccaaaa ttcctaacct gctggtgaac
     5401 ggttcttccg gtatcgccgt aggtatggca accaacatcc cgccgcacaa cctgacggaa
     5461 gtcatcaacg gttgtctggc gtatatcgat gatgaagaca tcagcattga agggctgatg
     5521 gaacacatcc cggggccgga cttcccgacg gcggcaatca ttaacggtcg tcgcggtatt
     5581 gaagaagctt accgtaccgg tcgcggcaag gtatatatcc gcgcccgtgc tgaagtggaa
     5641 gttgacgcca aaaccggacg tgaaaccatt atcgtccacg aaattccgta tcaggtaaac
     5701 aaagcgcgcc tgatcgagaa gattgcggaa ctggtaaaag aaaaacgcgt ggaaggcatc
     5761 agcgcgctgc gtgacgagtc tgacaaagac ggtatgcgca tcgtgattga agtgaaacgc
     5821 gatgcggtcg gtgaagttgt gctcaacaac ctctactccc agacccagtt gcaggtttct
     5881 ttcggtatca acatggtggc attgcaccat ggtcagccga agatcatgaa cctgaaagac
     5941 atcatcgcgg cgtttgttcg tcaccgccgt gaagtggtga cccgtcgtac tattttcgaa
     6001 ctgcgtaaag ctcgcgatcg tgctcatatc cttgaagcat tagccgtggc gctggcgaac
     6061 atcgacccga tcatcgaact gatccgtcat gcgccgacgc ctgcagaagc gaaaactgcg
     6121 ctggttgcta atccgtggca gctgggcaac gttgccgcga tgctcgaacg tgcgggcgac
     6181 gatgctgcgc gtccggaatg gctggagcca gagttcggcg tgcgcgatgg tctgtactac
     6241 ctgaccgaac agcaagctca ggcgattctg gatctgcgtt tgcagaaact gaccggcctt
     6301 gagcacgaaa aactgctcga cgaatacaaa gagctgctgg atcagatcgc ggaactgctg
     6361 cgtattcttg gtagcgccga tcgtctgatg gaagtgatcc gtgaagagct ggagctggtt
     6421 cgtgaacagt tcggtgataa acgccgcact gaaatcaccg ccaacagcgc agacatcaac
     6481 ctggaagatc tgatcactca ggaagatgtg gtcgtgacgc tctctcacca gggctacgtt
     6541 aagtatcagc cgctttctga atacgaagcg cagcgtcgtg gcgggaaagg taaatctgcc
     6601 gcacgtatta aagaagaaga ctttatcgac cgactgctgg tggcgaatac ccacgaccat
     6661 attctgtgct tctccagccg tggtcgcgtc tattcgatga aagtttacca gttgccggaa
     6721 gccactcgtg gcgcgcgcgg tcgtccgatc gtcaacctgc tgccgctgga gcaggacgag
     6781 cgtattaccg ctatcctgcc ggtgaccgag tttgaagaag gcgtgaaagt cttcatggcg
     6841 accgctaacg gtactgtgaa gaaaaccgtc ctcaccgaat tcaaccgtct gcgtaccgcc
     6901 ggtaaagtgg cgatcaaact ggttgaaggc gatgagctga tcggcgttga cctgaccagc
     6961 ggcgaagacg aagtaatgct gttctccgcc gaaggtaaag tggtgcgctt taaagagtct
     7021 tctgtccgtg cgatgggctg caacaccacc ggtgttcgcg gtattcgttt aggcgaaggt
     7081 gataaagtcg tctctctgat cgtgcctcgt ggcgatggcg caatcctcac cgcaacgcag
     7141 aacggttatg gtaaacgtac cgcagtggcg gaatacccga ctaagtcgcg tgcgacgaaa
     7201 ggggttatct ccatcaaggt taccgaacgt aacggtttag tggttggcgc ggtgcaggta
     7261 gatgactgcg accagatcat gatgatcacc gatgccggta cgctggtacg tactcgcgtt
     7321 tcagaaatca gcatcgtggg ccgtaacacc cagggcgtga tcctcatccg tactgcggaa
     7381 gatgaaaacg tagtgggtct gcaacgtgtt gctgaaccgg ttgacgagga agatctggat
     7441 accatcgacg gcagtgccgc ggaaggggac gatgaaatcg ctccggaagt ggacgttgac
     7501 gacgagccag aagaagaata attttacttc ttcatgccaa aagggagcta tctcccttgt
     7561 ttgaattgaa aagtccaggc tgcaaagtct gggcttttgt cgtattaggg cacggtaaag
     7621 tttggctgtg cccgtaaaaa atggctggct atacacaagg aatgtggcaa tgagtggtga
     7681 aaaaaaggcg aaaggctggc ggttctatgg tcttgtaggt tttggcgcaa tagtactgct
     7741 ttccgctggc gtctgggcgt tgcaatatgc tggcagtggg ccagaaaaaa cgttgtcgcc
     7801 gctggtggtg cacaacaatc tgcaaatcga tctcaatgag ccggacctct ttctcgacag
     7861 cgactctctg agccagcttc ccaaagatct cctcaccatt ccgtttctcc acgatgttct
     7921 tagcgaagat ttcgttttct attatcagaa tcatgccgat cgtctgggca ttgaaggcag
     7981 cattcgtcgc attgtctatg aacacgatct cacgctgaaa gataaactct tttcgtcact
     8041 cttagatcag cccgcgcagg cggcgctatg gcacgataaa caaggccatc tttcacatta
     8101 tatggtgctt atccagcgta gtggcttaag caagctgctg gagccattgc tgtttgccgc
     8161 taccagcgac agccagttaa gcaaaacgga aatcagtagc atcaagctaa ataatgaaac
     8221 tattcctgtt tatcagttgc gctataacgg caataacgcc ctgatgttcg cgacatatca
     8281 ggacaagatg ctggtgtttt ccagcacgga tatgctgttt aaagatgaag agcaggatac
     8341 cgaagctacg gcgatcgcaa gtgatttgtt gagcggtaaa aaacgctggc aagccagctt
     8401 tggcctggaa gagcgtactg ccggaaaaac accagtacgc cagcgcatcg tggccagcgc
     8461 caggttgctg ggttttggct accagcggtt aatgccttct tttgctggcg tacgcttcga
     8521 aatgggtaac gacggctggc acagttttgt ggcgttaaat gatgaatccg ccagcgtaga
     8581 tgccagtttc gattttacgc cggtctggaa cagtatgcct gccggggcca gcttctgtgt
     8641 ggcggtgccg tattcacacg gtattgccga agagatgctt tcgcacatca gccaggaaaa
     8701 cgacaagctg aatggggcgt tagacggtgc cgcggggctg tgctggtatg aagactcaaa
     8761 attgcaaacc ccgctgtttg tcggtcagtt tgatggcact gccgaacagg cgcaattgcc
     8821 agggaaactg tttacgcaaa atattggtgc gcacgaaagc aaagcgccag aaggtgtttt
     8881 gccggtaagc cagactcagc agggcgaagc gcaaatctgg cgtcgcgaag tgagttcccg
     8941 atacggtcag tatccgaaag cgcaggcggc gcaacccgat caattaatgt cggattattt
     9001 tttccgcgtg tcgctggcga tgcaaaacaa aacgctgctt ttctccctcg atgacacgct
     9061 ggttaataac gccctgcaaa cactgaataa aacccgcccg gcaatggtgg atgtaatacc
     9121 cactgatggc atcgttccgc tctatatcaa tccacaaggc gtggcgaaac tgctgcgtaa
     9181 cgaaacgttg accagtctgc cgaagaatct cgaaccggtt ttttataacg ccgcacaaac
     9241 tttattaatg ccgaagctgg acgctttatc tcaacaaccg cgttatgtca tgaagctggc
     9301 ccagatggaa cccggtgccg cctggcagtg gctacccata acctggcagc cgctatgagg
     9361 cacgggctgc tggcgctgat ttgctggctg tgttgtgttg ttgcccatag cgaaatgctg
     9421 aatgtcgaac aatccgggct gtttcgcgcc tggtttgtac gcattgcaca agaacagctc
     9481 cgccaggggc caagtccacg ctggtatcag caggattgtg cgggcctggt gcgatttgcg
     9541 gcgaacgaga cgctgaaagt tcacgacagt aaatggctaa aaagtaacgg tttatctagc
     9601 cagtatttgc cgccagagat gacgctaaca cctgaacagc gtcaactggc gcaaaactgg
     9661 aatcagggga acgggaaaac cggcccctac gtgaccgcga ttaatttgat tcagtacaac
     9721 agccagttta ttggccagga cataaaccag gcgctgcctg gcgatatgat ttttttcgat
     9781 cagggcaatg cccagcactt aatggtctgg atggggcgtt acgtcatcta ccacaccgga
     9841 agcgccacga aaactgacaa cggaatgcgc gcagtcagtc tgcaacaact tatgacatgg
     9901 aaggacaccc gatggatacc caacgattcc aatcccaatt tcattggcat ttatcgttta
     9961 aattttctgg cgcgatagca gcgtgcttgt ccctgtctct tgtgggaaca gggcttgcta
    10021 atgctgatga ttcgcttcct tccagtaact atgcgccgcc cgcaggggga acattctttt
    10081 tgcttgctga cagcagtttt agcagcagtg aagaggcgaa agtgcgactg gaagcgccgg
    10141 ggcgtgatta tcggcgctat cagatggaag agtacggcgg cgtggacgtt cgcctgtatc
    10201 gtattcctga cccgatggca tttttgcgcc agcagaaaaa cctgcatcgc attgtggtgc
    10261 aaccgcaata tctgggcgac gggctgaaca atacgctgac ctggctgtgg gataactggt
    10321 acggcaaatc tcgccgcgtg atgcagcgta ctttctcttc tcagtcacgg cagaatgtgg
    10381 ctcaggtttt acccgaatta caactcggta atgccattat taaaccttcc cgttatgtac
    10441 agaacaacca gttttcaccg ctgaaaaaat atcccctggt ggaacagttc cgttatccac
    10501 tatggcaggc taaaccggtc gagccgcagc aaggggtaaa actggacggc gcatccagca
    10561 atttcatctc gccgcagccg ggtaacattt atattcctct cggccaacaa gagccgggac
    10621 tgtacctcgt cgaggcgatg gttggtgggt atcgggcgac gacggtggtg tttgtttccg
    10681 ataccgtggc gcttagcaaa gtgtcaggca aagagcttct ggtgtggacc gcgggtaaaa
    10741 aacagggtga agcgaagcca ggctcagaga tcttgtggac tgacggtctt ggcgtgatga
    10801 cccgcggtgt gaccgatgac agcggtacct tgcagttaca acatatatcg ccagaacgtt
    10861 catacattct gggtaaggat gctgaaggcg gcgtttttgt ctccgagaac ttcttctacg
    10921 aaagcgaaat ctacaacacc cgcttgtata tctttaccga tcgcccgcta tatcgcgcag
    10981 gcgatcgtgt cgatgttaaa gtgatgggcc gcgagttcca cgatccgttg cattcatccc
    11041 ccatcgtcag cgccccggcg aagctttcgg tgctggacgc taacggcagt ctgttgcaaa
    11101 ccgtcgatgt cacgctggat gcgcgcaatg gcgggcaggg gagtttccgc ctgccagaaa
    11161 atgccgttgc cggaggttat gagttacgtc ttgcttaccg caatcaggtc tatagcagta
    11221 gttttcgcgt agccaactat atcaagccac atttcgagat tggtttagct ctcgacaaaa
    11281 aagagttcaa aactggcgaa gcggtcagcg gcaaactgca actgctctac ccggatggtg
    11341 agccggtaaa agatgcccgc gtgcagttaa gtttgcgcgc tcagcaatta tcaatggtcg
    11401 gtaacgattt gcgttatgcc ggacgtttcc ccgtgtcgct ggaaggcagt gaaacggtgt
    11461 ccgacgccag cggtcatgtg gcgttaaatc tccccgccgc cgataaaccg agccgctatt
    11521 tgttaaccgt ctccgccagt gacggcgcgg cgtatcgcgt caccaccacc aaagagatcc
    11581 tcattgaacg cggcctggcg cattactctt taagtaccgc cgcacaatac agtaatagcg
    11641 gcgagtcggt tgtgttccgt tatgccgcgc tggaatcttc aaaacaggtt cctgttacgt
    11701 atgaatggtt gcgtctcgaa gaccgcacga gccatagcgg agatctaccg tcaggcggca
    11761 aatcctttac cgtcaatttc gataaacctg gcaactacaa tctgacatta cgcgataaag
    11821 acggcttaat tctcgctggg ttaagtcatg ccgtcagcgg taagggcagc acggcgcata
    11881 ctggtacggt agatatcgtg gcggataaaa cgctgtacca gccaggcgaa accgcgaaga
    11941 tgctgattac ctttccggag ccaattgatg aagcattatt gacgctggaa cgcgatcgcg
    12001 tggaacagca gtcgctgctt tcgcatccgg caaactggct aacgctacaa cgtttaaacg
    12061 atacccagta tgaagcccgg gttccagtga gcaattcctt tgcgcctaac atcacttttt
    12121 cggtgctgta tacccgtaat ggtcagtaca gttttcagaa cgccgggatc aaagttgccg
    12181 ttcctcagct ggatatccgg gtgaaaacgg acaaaaccca ttaccagcct ggtgaactgg
    12241 tcaatgtcga attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag
    12301 gcgtggtcga tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat
    12361 ttttctatcc gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct
    12421 acgaccaggc actctccagc gagccggttg cgcctggcgc aactaaccgc agcgagcggc
    12481 gagtaaaaat gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt
    12541 cactcacaac cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa
    12601 cccgctggcg tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg
    12661 cttatctgcg ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg
    12721 tgggcgacaa accgtcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg
    12781 cgctggtgac taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg
    12841 cgaattatat ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc
    12901 aacaaaatgg gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gataatagct
    12961 ggcccgttga acagcagaaa aatgtcatgc tcggcggtgg cgataacgcg ctgatgttgc
    13021 ccgagcaggc gagcaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca
    13081 acaatcttga tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc
    13141 tgatcccgct cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg
    13201 acattcgtca gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag
    13261 cgcgctttac ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat
    13321 ggtacgccga ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc
    13381 agcatatgct cgacagttat gccgagcagg cagataacat gccgttattg catcgggcgc
    13441 tggtgctggc gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaagggttgg
    13501 atgaagctat cgcccggcgc ggaactaaaa ctgaagattt ctctgaggaa gccctccgcg
    13561 atatcaatga cagcctgatc ctcgatacac cagaatctcc actggcagat gcggtggcaa
    13621 acgtcttaac catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg
    13681 ttcagcaata tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg
    13741 tgctgctcaa tagcgggggt gacgctaccc aggcggctgc tattttaagt ggtttgaccg
    13801 ctgagcaatc tactattgag cgcgcgctgg ccatgaactg gctggcgaaa tatatggcga
    13861 caatgcctcc agttgttttg cctgcgcctg cgggcgcatg ggccaaacat aagttaactg
    13921 gagggggcga atactggcgt tgggttggtc agggcgtgcc ggacattctc tcttttggtg
    13981 acgaattatc gccgcaaaat gtgcaggtcc gctggcgtga accggcaaaa acggctcaac
    14041 aaagtaacat tccggtgacc gttgaacgcc agttgtatcg gcttatcccc ggtgaagaag
    14101 agatgagctt tactctgcag ccggtgacca gcaatgagat tgacagcgat gcgctgtatc
    14161 tcgatgaaat tacgcttacc agcgagcagg atgcagttct gcgctatggt caggtggaag
    14221 taccgctccc gccgggagcc gacgttgagc gcacaacatg gggcatttca gtcaataaac
    14281 ccaacgccgc gaaacagcag gggcaattgc tggaaaaagc gcgtaatgaa atgggcgaac
    14341 tggcctatat ggtgccggtg aaagaactga cgggaacggt cactttccgc catttgctgc
    14401 gcttctcgca aaaagggcaa ttcgttctgc ctcctgctcg ttatgtgcgt tcctatgcac
    14461 ctgcgcagca aagtgttgcg gcagggagcg aatggcccgg gatgcaggtg aaataagtga
    14521 actggcgcag aatcgtctgg ctattggcgt tggttactct gccaacgctt gcggaggaga
    14581 cgccgttaca actggcactg cgtggtgcgc agcacgatca actgtatcag ctgtcgtcat
    14641 caggcgtgac caaagtctca gcattacctg actcgctcac cacgccgtta gggagtttgt
    14701 ggaaactgta tgtttacgcc tggctggagg atacccatca accggagcag ccctaccagt
    14761 gtcgcggaaa ctccccggaa gaggtttatt gttgtcaggc tggggagagc ataacgcgcg
    14821 ataccgcgct ggtacgttcc tgtgggctct attttgcgcc gcagcggttg catattggcg
    14881 cagatgtgtg ggggcaatac tggcaacagc gtcaggcacc tgcgtggtta gcgtctctta
    14941 ctacgctgaa gccagaaacc tcggtaacgg ttaaatcgct gcttgattca ttagcgacgt
    15001 tacctgcgca aaacaaagct caggaagtct tactggatgt ggtgctggac gaggcgaaaa
    15061 tcggtgtcgc atccatgctc ggtagtcggg ttcgggtgaa aacctggagc tggtttgccg
    15121 atgataaaca ggaaatccgt cagggtggtt ttgccggctg gttaaccgat ggtacaccgc
    15181 tgtgggttac tggcagtggc accagcaaaa cggtactgac ccgttatgcg acggtgctta
    15241 atcgggtttt gccagtgccg acacaagttg ccagtgggca atgtgtagag gtggagctat
    15301 ttgcccgcta tccgctgaag aaaatcacag cggaaaagag tacgacggca gtaaaacccg
    15361 gcgtacttaa tgggcgctat cgtgtgacat ttgccaatgg taaccacatt acgtttgtca
    15421 gccacggtga gacgacctta ctgagcgaaa agggcaaact gaaattgcag tcgcatctcg
    15481 atcgtgaaga gtacgttgcc agagttctgg atcgcgaggc gaaaagtacg ccccctgagg
    15541 cagcgaaagc catgaccgtg gcgattcgca cttttttaca gcaaaacgcc aatcgggaag
    15601 gcgattgctt gaccatcccc gacagcagcg ccacgcagcg tgtttctgct tcgccagcga
    15661 ccacgggggc gcgaacgatg accgcctgga cgcaggatct catctatgcg ggcgatccgg
    15721 ttcattatca cggcagtcgc gctaccgaag ggacgctttc ctggcggcaa gccatggccc
    15781 aggcggggct gggagagcgt tacgaccaga tactcgcgtt tgcttatccc gacaacagcc
    15841 ttagccgctg gggagcgcca cgctcaacct gtcagttatt acccaaagcg aaagcctggc
    15901 tggcgaaaaa aatgccgcag tggcggcgta tattacaagg cgagacgggg tacaacgaac
    15961 cagacgtgtt tgcggtctgt cgtttagtct caggtttccc ctataccgat cggcagcaga
    16021 aacggctgtt tattcgtaac ttctttacgc ttcaggatcg gctggattta acccatgagt
    16081 atctgcacct ggcctttgat ggttatccca ccgggcttga tgagaactat atcgaaacgc
    16141 tgacccgaca attattaatg gactaatgct atgcgaaaga tttttcttgc gttgttactg
    16201 gtggcgcttt cgcctgtcgc tcacagtgaa ggtgtgcagg aagtcgagat tgacgcaccg
    16261 ctctcaggct ggcatccggc agagggcgaa gatgccagtt tttcacaatc gatcaattac
    16321 ccggcgtcgt ccgtcaacat ggcagatgat caaaatatct cagcgcagat ccgcggcaaa
    16381 ataaagaatt atgctgcggc aggtaaagtt cagcagggcc ggctggtggt caacggtgcc
    16441 agtatgccgc agagaattga atccgatggt tcatttgcac gcccttatat tttcactgaa
    16501 ggcagcaaca gcgtgcaggt catcagcccg gatgggcaaa gccggcaaaa aatgcagttt
    16561 tactcaacgc cgggtacagg aacgattcgt gcacgtttac ggctggttct ctcgtgggat
    16621 acggacaata ccgacctcga tcttcacgtt gtcacgcccg atggcgaaca cgcctggtac
    16681 ggtaacaccg tgctgaaaaa cagtggtgca ctggatatgg atgttacgac ggggtacgga
    16741 cccgagattt tcgccatgcc agcgccagtt cacgggcgct atcaggtgta tatcaactac
    16801 tatggcggac gtagcgaaac ggaattaacc accgcccaac tgacgctgat caccgatgaa
    16861 ggatcggtca atgagaaaca ggaaacattt attgtaccga tgcgtaatgc tggcgagctg
    16921 acgctggtga aaagttttga ctggtgatcc atcccttcac acaacctggc tctttctttt
    16981 ttcagataag actgctgggc gatctgccga cgccaggata taaccattaa caagcgtcgg
    17041 cagattggtc atttcttatt tttcgtactg agaactgtcc atttactccg gctttcctgg
    17101 ttaaggcaat ggtttctaag cgatacgcat tattgcaatg gtgacagaac attgcggtaa
    17161 cacgctttta ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc
    17221 tttgaaatac cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc
    17281 attggcgtta gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc
    17341 gttacatcag cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca
    17401 gcgctttatt caacgcacct ctgatgtgat gaaagagctg aagtacatcg ctgaaaatcg
    17461 cttatcggca gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc
    17521 tgcgtttgaa ccgctgtttg ccgactctga ttgttccgca atgagtaaca cctggcgagg
    17581 ttctctggag tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta
    17641 cgatctcaac cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct
    17701 gcgtgatatg ccagtggaac gcgataccgc gttgaaagcg ttgcatgaac gcatcaataa
    17761 atatcgaaat gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc
    17821 gcgccctggc gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca
    17881 ggcgcttttg ggtgtcgagc agaccatccg gatggagaat tttttcttac cgggtacgtt
    17941 gccgatgggg gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc
    18001 agaaagtaaa attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac
    18061 ggaagggttc cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt
    18121 gtattcggtg ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat
    18181 tttgctgaat gtgcttgccg gagcagcatt gtttactctc gcgcggatgt acgagcgacg
    18241 tattttcatt ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agtttaatcg
    18301 caagattgtc gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa
    18361 tattttaagt aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca
    18421 acgactgacg caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa
    18481 caataccaat ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat
    18541 ttgtgtgctg gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc
    18601 acaagcagcg gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga
    18661 gctgcgaacg ccgctgtatg gcattatcgg taacctggat cttttgcaaa ccaaagagtt
    18721 accgaaaggc gtcgatcgtc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa
    18781 aattatcagc gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc
    18841 gcgtgagttt tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt
    18901 ggtacgcaag cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa
    18961 tggcgacccg atgcgtttac agcaggtcat ctctaacctg ctgagtaacg ccataaaatt
    19021 caccgatacc ggctgtatag ttttgcatgt tcgcgctgat ggcgattatc tctctatccg
    19081 tgttcgcgat accggcgtgg ggataccggc gaaagaagtg gtgcgcttgt ttgatccctt
    19141 cttccaggtc ggaacgggcg tacagcgtaa tttccagggg accggtctgg gtctggcgat
    19201 ttgtgaaaaa ctgatcagca tgatggacgg cgacatctcg gtagattcag aaccgggaat
    19261 gggcagccag tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg
    19321 cgtggaaggg ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca
    19381 gttcctggaa accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga
    19441 accgactccc gaagatgtgt tgatcactga cgaggtagtg agtaaaaaat ggcagggcag
    19501 agcggtagtg accttctgtc gtcgccatat tggtattccg ctggagaaag cgctagggga
    19561 gtgggtacac agtgtggctg caccgcatga gctaccggca ttgttggcgc gtatttattt
    19621 gatcgagatg gagagcgatg atcctgctaa cgctctgccg tcgacggaca aagcggtcag
    19681 cgataatgac gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct
    19741 ggcagatcag ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gcgtcgatgc
    19801 gcttaatgta cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa
    19861 tatggatggt taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat
    19921 cggagtaact gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga
    19981 cagctgcctg tcgaagccag taacgctgga tgttataaaa cagacgctga cggtatatgc
    20041 cgagagggtc aggaaatcgc gggaatcgta ggccggataa ggtgtttacg ccgcatccgg
    20101 cattcagtgc aaatgccaga tgcgacgttg acgcgtctta tctggcctac tttaatgtaa
    20161 tctgttgaaa taatgggaat cgtaggacgg ataaggcgtt tacgccgcat ccggcaatcg
    20221 gtgcaaatgc cagataagac actaacgcgt cttatctggc ctacagatga ttagtcttta
    20281 tctgccggac ttaaggtcac agaagagaga taattcagca gggcgatatc gttctcgaca
    20341 cccagcttca tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc
    20401 tttttagcga tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct
    20461 tttggcgaga gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa
    20521 acgctttccg gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg
    20581 gtcggtgcac cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga
    20641 atcgccgggt tgttgttcat ggtcagaaca atgatcgaca ggcttgggaa atggcgcttg
    20701 atgtacttga ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta
    20761 atcaacacat gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat
    20821 tcgccgacaa cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact
    20881 atcggatggt catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta
    20941 cagcaagctc ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc
    21001 cttctcacga atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag
    21061 catggcaaat acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt
    21121 tgctgcttca gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata
    21181 atagccgctg gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc
    21241 ttcctgcgcc agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat
    21301 attgaagttg acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct
    21361 taaaagcaag ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc
    21421 ttgactaatt aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg
    21481 agtgacaata ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag
    21541 taaacgctct tcttcctctt caacttccgg gtcagctgcg agcattttga tatgcacaga
    21601 gtagcgtgta ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc
    21661 cagttgatcg ctcagccaga atgccagcgg gtccgccttg ccatagcgat cgttttgggt
    21721 ctggttgata aacgggaagt gcaaattatc catttcatga atgcttacgc cttctccggt
    21781 gtccagaatg cggaacgtca ggcggtcttc ggaggactca tcctgatcaa cctcaagggt
    21841 gatttttccc aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg
    21901 taatgcatcg cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag
    21961 ttgcagacct ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc
    22021 ctgcacggag aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa
    22081 ctgtatttca tcgaccaatc gcaccagcac atctgcctga ttcgccagtt gtttgctttc
    22141 cggggcgttg agtttagccg cgctctccgc cagggactgt gcgggttctt tcagcgcatc
    22201 gccaatgttt ttcataaagg tcatccgccc ctgctggttt ttctcataca gacgctgcgc
    22261 ctgcttgagt ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg
    22321 tgtgcgcggc gcaacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt
    22381 cgcctgaata atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg
    22441 cggcagcaaa tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg
    22501 aaccagcagg ccgagcggca gcagtgagac tatctcttca ttgattgccc gtaaaatgcg
    22561 caattcgtta ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg
    22621 gaatgtggta tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag
    22681 cagaatgttt tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccagtcg
    22741 catatcggta gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact
    22801 gacgctatcc gtcccttctt tctcattatc attgtttccc gttgccgtcg cgtctggctc
    22861 aaggcggaaa ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa
    22921 agccacgacc gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg
    22981 accgttctgc caggccaggc ggcgcatgtt agaaaagctt tcgcgttcat cgagggcgtt
    23041 ggcctgttgc aacatctctg cacgacgtga atcaacaatg tcgctgacgg tcgattcttt
    23101 aaatccggag gtgagatctt tgaggggcag ggttgagatc agcaccagac tgttatcctg
    23161 accattcagg taatacattg accacggtac attttctgcg ccccacaatg tgtccagata
    23221 ggtggacatc cgttgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc
    23281 ttccgttttg cggcgcggtt tttccagata gtagacatcc tgtttcaggc gcgtctcttg
    23341 taaaccttcg ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt
    23401 cacgtaacgc caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta
    23461 acttttatct ttcagccagg cgttaacggc gctttgtacc attacaccca tcgtcaccag
    23521 taacacaatg atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg
    23581 ggtcgtggcc gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga
    23641 agtgataggg cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat
    23701 tctctattct cctgtgatat ttatcatcct gtatgggggg aaatagatgt acaagcgcca
    23761 ttttgctgta caaataacct acgaaaagcc caacgaaatt aattgcaaat aaacgaaaat
    23821 tgctggaaat tatgctgatg ttaattattt gtgaaatagt taacaagcgt tatagttttt
    23881 ctctggtagc acagaataat gaaaagtgtg taaagaaggg taaaaaaaac cggatgcgag
    23941 gcatccggtt gaaatagggg taaacagaca ttcagaaatg aatgacggta ataaataaag
    24001 ttaatgatga tagcgggagt tattctagtt gcgagtgaag gttttgtttt gacattcagt
    24061 gctgtcaaat acttaagaat aagttattga ttttaacctt gaattattat tgcttgatgt
    24121 taggtgctta tttcgccatt ccgcaataat cttaaaaagt tcccttgaat ttacattttg
    24181 aaacatctat agcgataaat gaaacatctt aaaagtttta gtatcatatt cgtgttggat
    24241 tattctgcat ttttggggag aatggacttg ccgactgatt aatgagggtt aatcagtatg
    24301 cagtggcata aaaaagcaaa taaaggcata taacagaggg ttaataacat gaaagttaaa
    24361 gtactgtccc tcctggtccc agctctgctg gtagcaggcg cagcaaacgc tgctgaagtt
    24421 tacaacaaag acggcaacaa attagatctg tacggtaaag tagacggcct gcactatttc
    24481 tctgacgaca agtctgtaga tggcgaccag acctacatgc gtcttggctt caaaggtgaa
    24541 actcaggtta ctgaccagct gaccggttac ggccagtggg aatatcagat ccagggcaac
    24601 gctccggaaa gcgaaaacaa ctcctggacc cgtgtggcat ttgctggtct taaattccag
    24661 gacatcggtt ctatcgacta tggtcgtaac tacggcgtag tttacgacgt aacttcttgg
    24721 accgacgtac tgccagaatt cggtggcgac acttacggtt ctgacaactt catgcagcag
    24781 cgtggtaacg gcttcgcgac ctaccgtaac actgacttct tcggtctggt tgatggcctg
    24841 aactttgctg ttcagtacca gggccagaat ggcagcgtaa gcggtgaaaa tgatccggat
    24901 ttcactggtc atggcatcac caacaatggt cgtaaagctc tgcgtcagaa cggcgacggc
    24961 gttggcggat ctatcactta tgattacgaa ggctttggta tcggtggtgc gatctccagc
    25021 tccaaacgta cctgggatca gaacaacact ggtctgatcg gtactggcga ccgtgctgaa
    25081 acctacactg gtggtctgaa atacgacgct aacaacatct atctggctgc tcagtacacc
    25141 cagacctaca acgcaactcg cgtaggttcc ctgggttggg cgaacaaagc acagaacttc
    25201 gaagctgttg ctcagtacca gttcgacttc ggtctgcgtc cgtccctggc ttacctgcag
    25261 tctaaaggta aaaacctggg tgtcatcaat ggtcgtaact acgacgacga agatatcctg
    25321 aaatatgttg atgttggcgc gacctactac ttcaacaaaa acatgtccac ctatgttgac
    25381 tacaaaatca acctgctgga cgacaaccag ttcactcgtg acgctggcat caacactgat
    25441 aacatcgtag ctctgggtct ggtttaccag ttctaatctc gattgatatc gaacaaaggg
    25501 cctgcgggcc cttttttcat tgttttcagc gtacaaactc agttttttgg tgtactcttg
    25561 cgaccgttcg catgaggata atcacgtatg gaaataagct ttacccgcgt ggcactgctg
    25621 gctgccgcgc tcttctttgt tggttgcgat caaaaaccac aacccgccaa aacccacgct
    25681 actgaagtta ccgttcttga aggcaaaact atgggtacct tctggcgtgc cagcatcccg
    25741 ggcattgacg ccaaacgcag cgccgaactt aaagaaaaga ttcagaccca gctggacgcc
    25801 gacgatcagc tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt taacgactcg
    25861 caaagtttgt cgccgtggcc ggtaagtgaa gcgatggccg atatcgtcac cacctcgctg
    25921 cgcattggcg caaggaccga tggtgcgatg gatataaccg tcgggccgct ggtgaatctg
    25981 tggggctttg gcccggaaca acagccggtt caaattccga gccaggaaca gatcgatgcg
    26041 atgaaagcca aaaccggctt acagcacctg acggtcatta atcagtcgca tcagcaatat
    26101 ctgcaaaaag acctgccgga tttatatgtc gatctctcta ccgtcggcga aggttatgcg
    26161 gcggatcacc tggcacgctt gatggagcag gaagggattt cccgctatct ggtgtcggtg
    26221 ggcggcgcgc tgaacagccg tggtatgaac ggtgaaggcc agccgtggcg ggtagcgatt
    26281 caaaagccaa ccgataaaga aaatgcggtt caggccgtgg tggatatcaa cggtcatggc
    26341 attagcacct ctggcagcta ccgtaactat tacgaactgg acggcaaacg tctttcccat
    26401 gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt gacggtgatt
    26461 gctccgacgg cgctggaagc cgatgcctgg gatactggct tgatggtact cgggccggag
    26521 aaagccaaag aggttgttcg ccgggaaggg ctggcggttt atatgatcac caaagaaggc
    26581 gatagcttta aaacctggat gtcaccacag tttaaaagct tccttgtcag cgaaaaaaat
    26641 taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg gcagactaaa ggctatcctt
    26701 aaccagggag ctgattatga aaaacgccac atgcttaact gacgatcaac gctggcaatc
    26761 tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt ttcgccgtgc gcaccacagg
    26821 tatcttttgc cgtccgtctt gccgcgccag acatgccttg cgggaaaatg tctccttcta
    26881 cgcaaatgcc agcgaggcac tcgccgccgg atttcgcccc tgcaaacgct gccaacctga
    26941 taaagccaat gcccagcaac atcggttgga taaaatcacc cacgcgtgtc gactgctgga
    27001 acaggaaacg cctgtaacgt tggaagcctt agccgaccag gtggcgatga gtccatttca
    27061 tctacatcgg ttgtttaaag cgactaccgg aatgacgcct aaagcctggc aacaggcctg
    27121 gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga cgtctattct
    27181 taacgccgga ttccccgaca gcagcagtta ctaccgcaaa gccgatgaaa cgctgggcat
    27241 gacggctaaa caattccgtc acggtggcga aaatctggcg gtgcgttacg cgctggctga
    27301 ttgtgagctg ggtcgttgcc tggtggcaga aagcgagcgg gggatttgcg cgatattgct
    27361 gggcgatgat gacgcgacat taatcagcga gttgcagcag atgtttcccg ctgccgacaa
    27421 cgcgcctgcc gatctgacgt ttcagcaaca tgtgcgtgaa gtgatcgcca gcctcaatca
    27481 acgcgatact ccgctgacgt taccgctgga tattcgcggc actgcttttc agcaacaagt
    27541 ctggcaggca ctgcgcacga taccttgcgg tgaaaccgtc agttatcagc aactggctaa
    27601 cgccatcggc aaaccgaaag cggtacgggc cgttgccagt gcctgtgccg ccaacaagct
    27661 ggctatcgtt ataccctgtc atcgggtagt acgcggtgat ggatcgcttt caggttaccg
    27721 ctggggcgcg tcgcgcaaag cgcaactgct gcgccgcgaa gctgagaatg aggagaggta
    27781 atgttggatc tgtttgccga tgctgaaccg tggcaagagc cactggcggc tggtgcggta
    27841 attttacggc gttttgcttt taatgctgcg gagcaactga tccgcgatat taatgacgtt
    27901 gccagccagt cgccgtttcg ccagatggtc acccccgggg gatataccat gtcggtggcg
    27961 atgaccaact gtgggcatct gggctggacg acccatcggc aaggttatct ctattcgccc
    28021 attgatccgc aaacaaataa accgtggccc gccatgccgc agagttttca taatttatgt
    28081 caacgtgcgg ctacggcggc gggctatcca gatttccagc cagatgcttg tcttatcaac
    28141 cgctacgctc ctggcgcgaa actgtcgctg catcaggata aagacgaacc ggatctgcgc
    28201 gcgccaattg tttctgtttc tctgggctta cccgcgattt ttcaatttgg tggcctgaaa
    28261 cgtaatgatc cgctcaaacg tttgttgttg gaacatggcg atgtggtggt atggggcggt
    28321 gaatcgcggc tgttttatca cggtattcaa ccgttgaaag cggggtttca tccactcacc
    28381 accgactgcc gctacaacct gacattccgt caggcaggta aaaaagaata aaaataagaa
    28441 ttattattgc tgtgcgcgcg aagatgttta aactgcgggc tgtaattcat tgtccgggtt
    28501 ttctgcatgg aacttcttgt acttgtctgg cggcagtatc gctggccatt tatcagtgtg
    28561 atggcgctaa gcctcgccag tgcggcttta ggcattggct taattgcttt tatcaatcag
    28621 cgccttatcg aaacggcgga taccagtctg ctggtgttgc cggagtttct gggattattg
    28681 ctgctgttga tggcagtcac tctcggatcg caactggcgc tcaccacttt ggggcatcac
    28741 ttcgtttacc gactgcgtag tgaatttatc aagcggattc tggataccca cgtcgagcgc
    28801 attgaacaac tcggtagcgc ctcgttgctg gcgggattaa ccagcgatgt gcgcaatatc
    28861 accattgctt ttgtgcgtct gccggaactt gtgcagggga tcattctcac tatcggttcc
    28921 gcggcgtatc tgtggatgct gtcgggcaaa atgttgctgg taacggctat ctggatggcg
    28981 atcaccatct ggggcggttt tgtgctggtg gcgcgggtgt acaaacatat ggcgaccctg
    29041 cgtgaaaccg aagacaagct gtacacggat tttcaaactg tacttgaagg gcgcaaagag
    29101 ctgactctga accgggaacg cgccgagtat gtgtttaaca acctctacat tcctgatgcg
    29161 caagagtatc gccatcatat tatccgcgca gacaccttcc atcttagtgc cgtgaactgg
    29221 tcaaacatca tgatgctggg cgctatcggc ctggtgttct ggatggcgaa cagtctcggt
    29281 tgggctgata ccaacgttgc cgcgacctat tcgttgacgc ttttattcct gcgtacgccg
    29341 ctgctttcgg cggttggcgc attgccgacg ctgctgacgg cgcaggtggc gtttaacaag
    29401 ctgaacaaat tcgcgctcgc gcctttcaaa gcagagtttc cgcgcccgca ggcgtttccc
    29461 aactggcaaa cgctggagct gcgtaacgtg acgtttgctt atcaggataa cgcgttttcc
    29521 gttggtccga ttaatctcac catcaaacgt ggcgagctgc tgtttctgat tggcggcaac
    29581 ggtagcggaa aatcgacgct ggcgatgttg ttgacgggct tgtatcagcc acaaagcggc
    29641 gaaatcttgc tggatggcaa acctgtcagc ggcgaacaac cggaagatta tcgcaaactg
    29701 ttttcggcag tatttaccga tgtctggctg tttgatcaac tgctggggcc ggagggtaaa
    29761 cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc tgaaaatggc tcataagctt
    29821 gagttaagca acgggcgtat tgttaacctg aagttatcaa aagggcagaa aaaacgcgtg
    29881 gcgctgttgc tggcgctggc agaagaacgc gatattatcc tgctggatga atgggcggcg
    29941 gatcaggatc cacacttccg tcgtgagttt tatcaggtgt tgctgccgct gatgcaggag
    30001 atgggtaaaa ctattttcgc catcagtcat gatgatcatt actttatcca cgccgaccgc
    30061 ctgctggaga tgcgcaacgg gcaacttagc gagctgacgg gcgaagagcg cgatgccgct
    30121 tcgcgtgatg ccgttgcccg gacggcataa tattacgacg ctgacctggc ggactgctgc
    30181 cgccaggtca atatgctaaa aatccc
//
LOCUS       DAZFFG010000048        30017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFFG010000048 DAZFFG010000000
VERSION     DAZFFG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.048"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            86..1441
                     /locus_tag="ACHJ7J_002481"
     CDS             86..1441
                     /locus_tag="ACHJ7J_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFO5000702.1"
                     /translation="MARFQFKSRKNNGFIFLISFMVMGEATIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMVSAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            1550..22327
                     /locus_tag="ACHJ7J_002482"
     CDS             1550..22327
                     /locus_tag="ACHJ7J_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5000703.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHVTFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDTTGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLNVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQSDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHIQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPAGTAITVTLNG
                     QNYAATTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVASDDIVNAAEAGVAQIISGQVTGAAVGDTVTVTLGGTTYTTTVQANLSWSVS
                     VPAADIQALGNGDLTVSASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAEGTPLTVTINDVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAENPVSITHPVTVDLTPAAITINTIATDDVINAAEKGANLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLAALPEGSASVQASVSNINGNSASAVH
                     SYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGTTTAEAGQIVTVTLNSPTVQTYQ
                     ATVQADGSWSINIPAADLAALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINAAEHGQALVISGSSTGGEAGDVVSVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDVINATEKGAD
                     LQISGTSNQPAGTTITVTLNGQNYTATTDASGNWSTTVPASAVGALGEASYAVTANVT
                     DSAGNSNSVSHNVQVNTALPGVTLNPVASDDIINAAESGVAQTISGQVTGAAAGDTVT
                     VTLGGKTYTATVQGNLSWSVDVPAADIQAIGNGDLTVNASVTNGVGNTGSGSRDITID
                     ANLPGLRVDTVAGDDVVNSIEHAQALVITGSSSGLAAGAALTVVINNVTYGATVLADG
                     TWSLGVPAANVGNWPAGTVNITVSGATSAGNPVTITHPVTVDLAAVAISINTVSGDDV
                     INAAEKSADLTLSGSTSGVEPGQTVTVTFGGKTYTATVAGDGSWTTTVPAADLSALRD
                     GDATVQASVSTINGNTASATHAYSVDATAPTLAINTIATDDILNAAEAGNPLTISGSS
                     SAEAGQMVTVTLNGVTYTGTVQADGSWSVSVPTADLSNLTASQYTVSASVNDKAGNPA
                     STTHGLAVDLTVPVLTINTVSDDDIINAAEHGQALVISGSSTGGEAGDVITVTLNSKT
                     YTTTLDASGNWSVGVPAADVTALGSGPQTITAAITDAAGNSDDASRTVTVNLTAPTIG
                     INTIASDDVINATEKGADLQITGTSNQPVGTTITVTLNGQNYTATTDSSGNWSATVPA
                     SAVSALGEANYTVTANVTDTAGNSNSASHNVLVNSALPAVTINAVATDDIINAAESGN
                     AQTISGQVTGAAAGDTVTVTLGGNTYTATVQANLSWSVSVPAADIQALGNGSLTVNAS
                     VTNAVGNTGSGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSSGLTAGTA
                     LTVEINNVTYGATVLADGTWSLGVPAAEVSNWPAGTVDITVSGTNSAGTTSTITHPVT
                     VDLAAVAITINTLSGDDVINAVEKGETLVVSGSTSGVETGQTVTVTFGGKNYTTTVEA
                     NGSWTVNVPPADLAALPDGAGNVQASVSNINGNNAQADRAYSVDATAPLVTINTIASD
                     DILNVSEAGAGITISGTTTAQAGQTLTVTLNNNTYQTTVQADGTWSVNVPAADLSGLT
                     ASSYTVTATVSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVSGT
                     STGAAAGDVVTVNLNGKNYTTTLDASGNWSVGIPAADVTALATGSQTITASLSDRAGN
                     SDSTTHNVTVDLSGPTLTISIVSGDDIINNAEKTQDLTISGGSSGLAIGTTVTVMLNG
                     LAYSATTDSSGNWSVTVPASAVGALGEAVYQISASATDSAGNSGSTTHTVNVESLLPG
                     VIINTVAGDDIINAAEIVVAQTISGQVTGTAVAGNTVIVTIGGNQYNATVQSDLSWSV
                     SVPANVLQALGNGELTISASVTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSI
                     EHTQALVVTGSSTGLAAGAALTVVINSVTYGATVLADGSWSVGVPAADVTNWPAGTVN
                     IAVSGTNTAGTTTSITHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVE
                     AGQTITVIFGGKSYTTTVAADNSWGLTIPAADLATLPDGAANVQASVSNVAGNSAQAT
                     HAYSVDATAPSVTINTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGN
                     VQADGSWSVSVPTGDLANLTASSYTVNASVSDKAGNPASAMHNLTVDLVAPVVTINTV
                     AGDDIINATEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVI
                     SALAQGDVTITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINATEKGADLA
                     ISGTSDQPAGTQITVTLNGQNYTTTADASGIWSVTVPASAVGTLGEATYTVTASATDI
                     DGNSGSASHNVQVNTALPGVTINVVATDDIINAAEAGAGQTISGQVTGAAAGDTVTIT
                     LGGATYTATVQANLSWSVDVPASALQALGNGELTISASVTNSVGNTGNGTREITIDAN
                     LPGLRVDTVAGDDVVNIIEHGQALVITGSSSGLATGSNVTLTINGQTYVAAVLADGTW
                     SVGVPAADVSAWPAGLVTITASGNTSAGNPVSLTHPVTVDLSAVAVSINAITADDVIN
                     AAEKGAALTLSGSSSGVEAGQTVTVTFGGKTYTASVAANGSWSTTVPAADMAALRDGD
                     ASAQASVSNVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTA
                     EAGQTVTVTLNGANYIGTVQTDGSWSVSVPSADLSALTASNYTVSAAVSDKAGNPASV
                     NHNLTVDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFT
                     TVLDASGNWSVGVPASVVSALANGTVTINASVTDAGGNSGSATHQVTVNTGLPTITFN
                     AISGDNILNADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVS
                     DLAALGQANYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGAD
                     QTISGVVTRAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASV
                     TNANGNTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVL
                     TVTINSVAYSATVQADGSWSVGIPAANVSAWPAGPLTVEVAGQSSADNPVSVSHPFTV
                     DLTAVAISINTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAAN
                     GSWSKTVPVADLATLPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDD
                     ILNATEAGSPLTISGSSTAETGQTVTVTLNGATYTGTVQADGGWSVSVPTSALGALNA
                     SNYTVSATVNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTS
                     TGGEAGDVVSVVLNGKTYTTTLDASGNWSVGVPAADVAALGSGAQTITASVSDRAGNS
                     DDASRTVTVSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQN
                     YSATTDASGNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGIT
                     INPVATDDIINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQPDLSWNVSVP
                     AADWQALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHA
                     QAQVITGSSSGFAAGTALTVVINNQTYAATVLANGSWSVGVPAADVSNWPAGTLNITV
                     SGANSAGTQTSITHPLTVDLTTVAISINAITPDDVINAAEKGAALMLSGSTSGVEAGQ
                     TVTVNFGGKTYTSTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEY
                     SVDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQA
                     DGSWSLTLPASDLTALANNGYTLTATVSDLAGNPGSASKGVTVDTTAPVISFNTVAGD
                     DVINNVEHTQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGL
                     ADGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTLSG
                     TGTNFATGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSA
                     GNSATASRSVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFG
                     GKTYTATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAP
                     TVTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWSATVGSGGSWSVF
                     IPAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAE
                     HGSSLVISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVI
                     NASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSL
                     TAALASNETAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGA
                     TDSQNVVIDTIAPDPAVKTIAISAITTDTGLITNDFVTSDTTLAVSGTLGAALSVGEF
                     AQISIDGGTTWQNLSVSGLTWTYLDGRTLSDGNYNYQVRVIDTAGNIGATASQIVTVD
                     TTAPLASKTIAIAGISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVT
                     WTTLTVIGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSI
                     TITGISDDTGTSSTDFITSDTSLTVRGVLGAALGANEFAQISTDNGATWVNVTVAADG
                     LNWSYVDGRMLTNGTTTWQVRVVDLAGNVGATGSQSAQIDTVNPAQVLTIASISTDTG
                     SSATDFITSDTTLTLTGSLGAGLASGEVAQISLDGGATWTTLTTNGTQWTYTDSRTLT
                     DGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLNSSQAT
                     DDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSAR
                     VVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVV
                     INGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNANI
                     SNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKT
                     ALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTV
                     GTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATV
                     GGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTN
                     TFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAV
                     GATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVGGASNWTQSALGGSQSGT
                     TSGVAAMDYDWDGAVDVLVTKQSGSVFLIRNTNTVSYGTSLHLRITDPNGINVYYGNT
                     VKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNID
                     QTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGW
                     VYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFN
                     DTLTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTW
                     EGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQ
                     IDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            22331..24493
                     /locus_tag="ACHJ7J_002483"
     CDS             22331..24493
                     /locus_tag="ACHJ7J_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO5000704.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADVEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWNLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAHLIEQLKQWIGHRTLVIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTTRRVVLQEKNKGSAA"
     gene            24490..25665
                     /locus_tag="ACHJ7J_002484"
     CDS             24490..25665
                     /locus_tag="ACHJ7J_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO5000705.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLNVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            25662..26069
                     /gene="cueR"
                     /locus_tag="ACHJ7J_002485"
     CDS             25662..26069
                     /gene="cueR"
                     /locus_tag="ACHJ7J_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO5000706.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(26070..27293)
                     /locus_tag="ACHJ7J_002486"
     CDS             complement(26070..27293)
                     /locus_tag="ACHJ7J_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000707.1"
                     /translation="MLFPFRKPEVRLLKNTAAGVIFSVKSGKGFLRQCVLYDPHPYGL
                     IRSLCWQDVPVIRFWSEAGCPTCAEFVYSGFADDEQGAARFLSALENWNRPWSGFTDA
                     FAALTPLFSCLADGYYLLEDRELYPTDGNGHFFWAATDHSASNPATVAVWDPEYCYFC
                     NTAPCFLLPGQPPSHFNPERVTFYRDKPDARALAWYLPDSYLCVLLDGHHKATAAALE
                     GRPLKTLVLSTATRFNDEQQTLVFPGGECLHKTELLCHVPKLTEWKTLPSGAWESFGP
                     DKHISPSETWSEELQQSVSRYPSLDQAWQIVEAGNLSETRIKSMIQQGLGEDEKADVI
                     LQALFFTHSPLFIDFARFVISYPAYVSYRPLTFRLMAQNRTPQADAFFLDFAINDDGE
                     RPELTKIMDDYFRKR"
     gene            complement(27382..27588)
                     /locus_tag="ACHJ7J_002487"
     CDS             complement(27382..27588)
                     /locus_tag="ACHJ7J_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000708.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(27585..28007)
                     /locus_tag="ACHJ7J_002488"
     CDS             complement(27585..28007)
                     /locus_tag="ACHJ7J_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000709.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGVTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKIALMKEGQFVGYLK"
     gene            complement(28093..29142)
                     /locus_tag="ACHJ7J_002489"
     CDS             complement(28093..29142)
                     /locus_tag="ACHJ7J_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5000710.1"
                     /translation="MKADDAQHAITVAQTDIDANKAAIADTRNDVSAVQSDVTNIKGD
                     VTHAQSTADHANANANTALINGVKLSGAVTENKNNIEQNRSDIADQQQQLDETRKSVA
                     ATGDVQTAARYQSMIDARQTAAANAQQEQADTQQQQMDDQQKQIDATQKTVSALGDAQ
                     TNAHYQEMVNAGLKAQNDANARTAAEQKQKIDTLATNQATQQHIDSVQYGEQIQRLAQ
                     DSTQTHEQIDSLTQDVTQTHQQLSNTQKRVADNSQQITTLNNHFSSLKNEVEDNRKEA
                     NAGTASAIAIASQPQVKTGDLMMVSAGAGTFNGESAVSVGTSFNAGTHTVIKAGVSAD
                     TQSDFGAGVGVGYSF"
BASE COUNT         6820 a         8668 c         8801 g         5728 t
ORIGIN      
        1 tgcacaagtt attacccttc ttaataaaat gagactggtt ataaaaacca gttaaaataa
       61 tgacctggaa ggaagtgact tatctatggc gcgattccag tttaaaagcc gtaaaaataa
      121 tgggtttatt tttttaataa gttttatggt gatgggagaa gctacaattg ctgcaccgct
      181 gccacaatgg gcgaacgccc cagcggtaac gccagttgcc caattatcct tacaggaaag
      241 tatattacgc gcctttgcgc gaaatcccga cgtcactcaa caggcggcgc agataggcat
      301 tggcgaagcg caaattgatg aagccaaaag tgcctggtat ccgcacgttg gattaaccgg
      361 caacgcgggg ccgtcccgac aaacggactc cagcggcagg cttgataaca acgtttcgta
      421 tggcataacc ctgacacagc tggtgtatga ctttggtaaa actaacaacg atatcaatct
      481 gcaaactgcc gcccgtgaca gctaccgctt taaattgatg gcaaccttaa ccgatgttgc
      541 agagaaaacg gcgactgcct atatggaagt cagtcgttat caggctttgt gcgatgcggc
      601 tcaacgcaat attcactcgc tggaaaacgt ctacaacatg gcggcattgc gcgctaacgc
      661 tggcctgaac tcatcgtcgg atgaattaca ggcccagacg cgtattgccg gaatgcgctc
      721 aacgctggag caatatcagg cgcagatggt gagcgccaaa gcgcaactgg cggtgctcac
      781 tggcgtacag ccggaggcga tagccgcgcc accggctgaa ttagccgagc agccggtatc
      841 gctaaaaaat attgattacc agtccatccc gctggtgctg gcggcagaaa acttacgcca
      901 gtcagcacag tacggcgtgg aaaaaacgaa agcgcaatac tggccaacgc tcagtattca
      961 ggggggtaaa acgcgctacc agaccagcga ccgctcgtat tgggatgatc agctacaact
     1021 gaacgttaac gcgccgctgt atcagggcgg cgcggtttct gcccaggtgc aacaggccga
     1081 agggcaacaa aaaatctctg cctcgcaggt cgaacaggca aaactggatg tgctgcaacg
     1141 agcgtctgtg gcatatgcga actggaccgg cgcacggggt cgtgaagaag ccggtttagc
     1201 gcaatccgaa agtgcgcaca aaacgcgaga tgtgtaccaa aatgaatata aattgggtaa
     1261 acgcagtctg aatgatctat taaccgtcga gcaagatgtc tttcaggcgc aatcggctga
     1321 aataaatgcc aattatgatg gctgggtcgc cgccgtaaat tatgccgctg cggtgaataa
     1381 ccttattcca ttagcgggaa ttaaacaggg cttatacaac gacttacccg acttgaaata
     1441 atcctgccat tattaatatc aggtatgcct cgttgttaag tgattacctt tgtcttattt
     1501 agataaatgg actcgctaag gatgtgcgcc atttatcagg aatgccatta tgagcctgat
     1561 tattgatgtt atttcgcgta aaacatccgt caaacaaacg ctgattaatc ctggcgacgt
     1621 cacggttgtt atttatgagc cttccgtggt gcaggttcat gctcaggcct ctgccgttgc
     1681 gcgttacgtc cgtgaaggaa atgacctgct gatctatatg caggacggca cggtgatccg
     1741 ctgcaacggt tatttcctgc aagcggcgaa tacagctgaa caatcggaac tggtgtttgc
     1801 cgatgggcaa cagctaaccc atgtcacctt cgccgatact gctgcgggtg gattagcccc
     1861 cgtagaactg actgcccaga ccactgcgat tgaaagcatt gcgccatttc ttgataccgt
     1921 tgctcagacc agcgccttcc cgtggggttg gctggcgggg gcggcggtag gtggcggtgc
     1981 gcttggtgca ctgctggcaa gcggtggcga tggcgactcg aaaacagaag tgattaataa
     2041 ccctacgcca cctgctgagc ctggcaacgc cacaccatca tttttagtta ccgataatca
     2101 gggcgatcag cgcggcattc tcgccaccaa tgacatcacc gatgacacca cgccaacctt
     2161 tagcggcagc gggcaggcgg gggcgactat tcagattaaa gacagtaacg gcaatactat
     2221 tgccagtact caggtagaca acaacggtca ctggagtgtc tcgttaccca cgcaaagtgc
     2281 aggtgaacat acctggtcag tggtgcaaat tgtcggcagt accatcactg acgccggttc
     2341 gataacgtta accatcgaca atagtcaggc cagcgtgcag gttgccacca ccgcaggcga
     2401 taacattatt aacgccagcg aacaggccgc cgggtttacg ctttctggca ccagtagcca
     2461 tctggcgcag ggaacagaac tcaccgtcac acttaacggc aaaacctaca ccaccagcgt
     2521 aggcgctaac ggtgcctgga gcgtgcaggt gccgaccgcc gatgcacagg cgttaggcga
     2581 agggaatcag gcggtgctgg tcagtgggaa agacaccaca ggcaatacgg tcaccggcgc
     2641 gcagctgcta acggtcgata cccaaccgcc aacgcttgcc atcaacacca tcgctcagga
     2701 caacatcatc agtgctgcgg aacataacgt cgcgctggta ctgagcggca cgtcgaatgc
     2761 agaagcgggg caaaccgtaa cactgaacgt caacgggaaa agccatacag caaccgtcgg
     2821 tagcgacgga acctggcaag tgacgctgcc tgccacggaa gtccaggcac tggcggaggg
     2881 taattacgct gtcaatgcca gtgtcagcga tcgggcaggg aacaccacca gccacagcgc
     2941 gaatttcacg gtagacacct cagcacccgt ggtcagtgtt aataccgtgg cgggcgacga
     3001 tattcttaat aatgccgagc aggccgtcgc gcagatcatc tccggacaag ttagcggtgc
     3061 ttctcctggc gatacggtaa cggtgaaatt gggcactcat gtcctgacgg gcatcgtgct
     3121 ggcagatggc agctggaatg tggcgttgga cccggcggta acccgcacgc tggatcgcgg
     3181 agccaatacg attttcgtca ccgtgacaga tgctgcagga aatactggcg cggcgtctcg
     3241 agcaatcacg ctggtcggtg tttctccgtt gatcaccatt aacaccgtct ccggcgatga
     3301 cattatcagt ggcgcagaaa aaggtgcgcc actgaccctt accggtagca ctcaacaggc
     3361 tgagacagga caaaccgtca cagtaaccct ggctggacag agttttacca ctaccgtgca
     3421 ggccgatggc tcctggagtc tgacggtacc tgccgccgcg atgggaaatc tgcctgacgg
     3481 cgcggtggcg attaccgctt ctgtgacgga tctcagcggc aataccggca acacttcccg
     3541 caccattacc gtcgatagcc aggccccggc cttaagcatc gatccactga ccgccgataa
     3601 catcattaac gccgcagaaa gcgggcagga tctgcccatc accggcacca ccgacgctca
     3661 gccggggcag acggtgacgg tgacgctgaa cgggcagacg tatcagggcg tcgtgcagtc
     3721 agacggcacc tggagcgtga ctgtgcccgc cgccaacgtg ggcgcactgg ctgacggcaa
     3781 cgctacggtc accgccagcg tgaacgatgt cgccggtaat ccgagcagcg tttcgcgcgt
     3841 ggcgctggtg gatgccacgc cgccggtggt caccattaat ccggtggcga ccgataacgt
     3901 catcaacacg ccggaacata tccaggcgca aattatcagc ggcacggtca ctggcgctca
     3961 ggcaggcgat atcgtcaccg tgacgctgaa taatgtggat tacaccacgg tagtggatgg
     4021 ttccggcaac tggagtctgg gcgttccggc ctcggtggtc agtgggctgg cggacggcag
     4081 ttatcctgtc agcgtctcgg taaccgacaa agccggaaac acgggcagcc agtcattgac
     4141 cgtcacggtc aataccgccg cgccccttat cggcattaac agcattgcgg gcgatgatgt
     4201 gattaacgcc agcgaaaaag gggccgatct ccagattacc ggcaccagcg atcagccagc
     4261 gggtaccgcc atcaccgtga cgctcaacgg gcaaaactat gcggcgacga ccgacgcctc
     4321 cggcaactgg agcgtcaccg ttccggcctc ggcggtaacc gcactgggcc aggccaacta
     4381 tacggtgacg gcggcggtga ccagcgatat cggcaacagc gccactgcca gccataacgt
     4441 gctggtcgac agcgcgctgc ccggcgtgac cattaacccg gtggcgagcg acgatattgt
     4501 taacgccgcc gaagcgggcg tggcgcaaat catcagcggg caggtgactg gagccgccgt
     4561 tggcgacacg gtaaccgtca cgcttggcgg tactacctac accaccaccg tgcaggccaa
     4621 tttaagctgg agcgtgagcg ttccggcggc agatattcag gcgctgggca atggcgattt
     4681 aacggtgagt gcctcagtca ccaatcaaaa cggcaacacc ggcagcggca cgcgggatat
     4741 caccatcgac gctaatctgc ccggcctgcg ggtcgatacg gtggcgggcg atgatgtggt
     4801 caatatcatc gagcacgggc aggcgctggt ggtcaccggc agcagctcgg ggcttgctga
     4861 aggcacgccg cttaccgtca cgattaacga tgtggaatac accactgcgg tgcaggccga
     4921 tggtagctgg agcgtgggcg tcacggcggc gcaggttagc gcctggcctg cggggacggt
     4981 taatattgcc gtatccgggg aaagtagcgc cgaaaatccg gtgagcatta cgcatccggt
     5041 tacggtcgat ctcactccag cggcgattac catcaacacc atcgccacgg acgacgtgat
     5101 taacgccgca gaaaaaggcg ctaatttaac gctttccggc accaccacta acgtagaacc
     5161 cggtcaaacc gtcaccgtta cctttggcgg gaaaaattac actgccagcg tagcgagcga
     5221 tggtagctgg actgccaccg ttcccgctgc cgatctggcg gcattaccgg aaggcagcgc
     5281 ctccgtgcag gccagcgtca gcaatatcaa cggcaatagc gcctcggcgg tgcatagcta
     5341 cagcgtcgac agcagcgcgc caaccatcat tatcaatacc gtcgccagcg acaatatcgt
     5401 caacgccagc gaagccgatg cgggcgtgac ggtgagcggc accaccaccg ccgaagcggg
     5461 gcagattgtt acggtaacgc tcaatagccc gaccgttcag acctatcagg caacggtaca
     5521 ggccgatggc agctggagta ttaatattcc ggcggcagat cttgcggcat tgacagatgg
     5581 cagccacacc ctgacggcga cggtcaatga caaagcgggc aacccggcga gcaccacgca
     5641 taatctggcg gtggatctca ccgttccggt acttaccatc aacaccattg cgggcgacga
     5701 tattattaac gccgccgaac acgggcaggc actggtgatt tccggttcca gcactggcgg
     5761 agaagcgggg gatgtcgtta gcgtcacgct aaacagtaaa acctacacca ccaccctgga
     5821 tgcttccggc aactggagcg tcggcgttcc ggcggcggat gtcaccgcgc tgggtagcgg
     5881 cccgcaaacc gtgactgcga ccgttaccga tgccgccggt aacagcgata gtgagacgca
     5941 cacggtcacc gttaacctta ccgcgccgac cattggcatt aacaccatcg ccaccgatga
     6001 cgtgattaac gccaccgaaa aaggcgcgga tctgcaaatt tccggcacca gtaatcagcc
     6061 cgcaggcact acaattacgg tgacgctgaa cgggcaaaat tacaccgcga cgaccgatgc
     6121 ttctggcaac tggagcacca cggtgccagc ctccgccgtc ggtgcgctgg gcgaagccag
     6181 ttacgcggtg acggcgaacg ttaccgacag cgcaggtaac agcaattccg tcagccataa
     6241 cgtgcaggtc aataccgcgc tgccgggtgt cacccttaat ccggtagcga gcgacgatat
     6301 tatcaacgct gccgaatcgg gcgtggcgca gactatcagc ggccaggtga cgggcgcggc
     6361 ggcgggcgat acggttaccg tgacgctggg cgggaaaacc tacaccgcta ccgtgcaggg
     6421 gaatctaagc tggagcgtgg atgttccggc ggcggatatt caggccatcg gtaatggcga
     6481 cctgactgtt aacgcctcag tgaccaacgg cgttggcaat accggcagcg gttcgcgaga
     6541 tattactatc gacgccaacc tgcctggcct gcgcgtcgat accgtggcgg gcgatgatgt
     6601 ggtcaatagc atcgagcacg ctcaggcgct ggtgataacc ggtagcagca gcgggctagc
     6661 ggcgggcgcg gcgctgacgg tggtgatcaa taacgtcacc tatggcgcga cagtattagc
     6721 cgatggcaca tggagccttg gcgttccggc ggcaaatgta ggcaactggc ctgcgggtac
     6781 ggtgaatatc acggtttcag gtgctactag cgcaggaaac ccggtcacca tcacccatcc
     6841 agtcaccgtc gatctggcag ctgtggcgat ctccattaac accgtttccg gcgacgatgt
     6901 gattaatgcc gctgaaaaaa gtgcagattt aaccctttcc ggcagcacct ccggcgtaga
     6961 gccggggcaa acggtcaccg tcacctttgg cgggaaaacc tacaccgcta ccgtagcggg
     7021 cgatggtagc tggacaacca ccgtgcctgc cgccgatctc agcgcgttac gcgacggcga
     7081 cgccacggta caggccagcg tcagcactat taacggcaac acggcttcgg caacgcacgc
     7141 ctacagcgtc gacgccacgg caccgacgct tgccattaac accatcgcca ctgacgatat
     7201 tctgaacgcc gccgaagcgg gcaatccgct aaccatcagc ggcagcagta gcgccgaagc
     7261 ggggcagatg gtaaccgtca cgcttaatgg cgtgacttac accggcaccg ttcaggcgga
     7321 cggtagctgg agcgtcagcg taccgacggc ggatctcagc aatctgaccg ccagccagta
     7381 caccgtcagc gcctcggtaa acgataaagc gggtaaccca gcgtccacca ctcacgggtt
     7441 ggcggtggat ctcaccgtgc cggtgctgac catcaacacc gtctccgacg atgacattat
     7501 taacgccgcc gaacacggac aggcgctggt gatctccggc tccagcacag gcggcgaagc
     7561 gggtgatgtc atcaccgtca cactaaacag taaaacctac accaccacgc tggacgcttc
     7621 cggcaactgg agcgtcggcg ttccggcggc tgacgtcact gcgcttggca gcggcccgca
     7681 aactatcact gcggcaatta ccgacgcggc aggcaacagc gatgacgcca gccgcacggt
     7741 caccgtgaat ctgaccgcgc caaccattgg catcaacact atcgccagcg atgatgtgat
     7801 taacgccacg gaaaaaggcg cggatctgca aattaccggc accagtaatc agccggtggg
     7861 caccaccatt acggtgacgc tgaacgggca aaattacacc gctacaaccg attcttccgg
     7921 caactggagc gccacggtgc cagcctcggc agttagcgca ttgggtgaag ctaactacac
     7981 ggtaacggca aacgtcaccg acacggcagg caacagtaat tccgccagcc ataatgtgct
     8041 ggtcaacagc gccttgcctg ccgttaccat taacgcggtg gcgaccgatg acattattaa
     8101 cgctgccgaa tcgggaaatg cgcaaaccat cagcgggcag gtgacgggtg ccgcggcggg
     8161 cgacacggtt accgttacgc tgggcggcaa cacctacacc gccacggtac aggctaattt
     8221 aagctggagt gtcagcgttc cggcggcgga tattcaggcg ctgggtaacg gttcccttac
     8281 ggttaatgcc tcggtcacca acgctgtggg caacaccggc agcggctcgc gcgatatcac
     8341 gatcgacgcc aatctgcctg gcttacgggt cgataccgtg gcgggcgatg atgtgattaa
     8401 cagcattgaa cacaatcagg cgctggtgat caccggcagc agcagcggat taacggcggg
     8461 aacggcgtta acggtcgaga ttaacaacgt tacttatggc gcgacggtat tagccgacgg
     8521 cacgtggagc ctcggcgttc cggcggcaga ggtcagcaac tggccagcgg gtacggtgga
     8581 tattacggta agcggcacca acagcgccgg aacaacctcc accattaccc atccggttac
     8641 cgtcgatctg gcggcggtcg ccatcaccat taacacactc tccggcgatg atgtgatcaa
     8701 cgccgttgaa aaaggcgaaa cgctggtcgt aagcggcagc accagcggtg tggaaaccgg
     8761 gcagacggtg accgtcacct ttggcggtaa aaattacacc actactgtgg aagctaacgg
     8821 tagctggacg gtgaatgtgc cgccagccga tctcgctgcg ctgccggacg gcgcgggcaa
     8881 cgtgcaggcg agcgtcagca acatcaacgg caataacgcc caggcggatc gtgcgtacag
     8941 cgtcgatgcc accgcgccgc ttgtgaccat caacaccatt gccagcgacg atatccttaa
     9001 cgtgagtgaa gcgggcgcgg gtatcaccat cagcggcacc accacggcgc aggccgggca
     9061 gacgctgacc gtcacgctca ataacaacac gtaccagacc accgttcagg cggatggcac
     9121 ctggagcgtg aatgttccgg cagcggattt aagcggatta accgccagca gttacaccgt
     9181 gaccgccacg gtgagcgaca aagcgggtaa cccggcaagc gccgaccacg cgctggcggt
     9241 ggacgttacc gcgccggatc tcaccattaa caccgttgcg ggcgatgaca ttatcaacgc
     9301 catcgaacac ggtcaggcac tggtcgtcag cggaaccagt acgggcgcgg cggcgggcga
     9361 tgtggtcacg gttaacctga acggtaaaaa ctacaccacc acgctggacg cctccggtaa
     9421 ctggagcgtg ggcattccgg cggcggatgt cacggcgctg gcaaccggta gccagacgat
     9481 caccgccagc ctgagcgatc gcgcgggcaa cagcgacagc actacccata atgtgaccgt
     9541 tgatctcagc ggcccgacgt tgaccatcag tatcgtctcc ggcgatgaca ttattaataa
     9601 tgcggagaaa acccaggatc tgaccatcag cggcggctcc agcggtctgg cgattggcac
     9661 cacggtcacc gtgatgttga acggcctggc gtacagcgcc accaccgata gttccggcaa
     9721 ctggagcgtt accgtgcctg cttcggcggt cggcgcactg ggtgaggcgg tgtatcagat
     9781 tagcgccagc gccaccgaca gcgcgggcaa cagcggcagc accacccata cggtgaatgt
     9841 cgaatcgctc ctgcctgggg tgatcattaa taccgttgcg ggcgatgaca ttatcaacgc
     9901 cgctgaaatc gttgtggcgc agaccatcag cggtcaggtc acgggaacgg cggttgccgg
     9961 gaatacggtg attgtcacca ttggcggcaa tcaatataac gccaccgtgc agtcagattt
    10021 aagctggagc gtcagcgtac cggcgaacgt tttgcaggcg ctgggtaacg gtgaactgac
    10081 catcagcgcc tcggtgacca attccgcaaa taataccggc accgcgacgc acgatatcgt
    10141 gatagatgcc aatctgccag gtctgcgcgt cgataccgtg gcaggcgatg atgtgattaa
    10201 cagcatcgag cacactcagg cgctggtggt taccggcagt agcaccgggc tggcagcggg
    10261 cgcggcgttg acggtggtta tcaatagcgt cacctacggc gcaacggttt tagcggatgg
    10321 tagctggagc gttggtgttc cggcggcaga tgtcacaaac tggcctgcgg ggacggtcaa
    10381 tattgccgtc tctggcacca acaccgccgg aacgacaacc agtattaccc atccggtgac
    10441 cgtcgatctc gcagccgtgg cgattaccat caacaccctt tccactgacg atgtgattaa
    10501 cgccgctgag aaaggctctg atttgcagct ttccggcacc acctccggcg tggaagcggg
    10561 gcagaccatt accgttattt tcggcggcaa aagctacacc accacggttg ccgcggataa
    10621 tagctggggg ctgacgatcc cagccgccga tctcgcaacc ttgccggacg gcgcagcaaa
    10681 cgtccaggcc agcgtcagca acgtcgcagg taacagcgcc caggcaacgc atgcttacag
    10741 cgttgatgcc actgcgccct ccgttaccat caacaccatc gccacggacg atattcttaa
    10801 cgccgccgaa gcgggatcgg cgctgaccat cagcggcacc agcacggcgg aagccgggca
    10861 gacggtgacc gtaacgctaa acggcgttaa ttacagcggc aatgtgcagg cagacgggag
    10921 ttggagcgtc agcgtaccaa ctggcgattt agctaacctt accgccagct cgtataccgt
    10981 taacgcctcg gtcagcgata aagccggaaa tccggcctcg gcgatgcata acctgacggt
    11041 ggatctcgtt gcgccggtcg ttaccatcaa cacggtagcg ggcgatgaca tcattaacgc
    11101 cacggaacac ggacaggcgc agatcatcag cggctcggca acgggcgcga ctaccggtaa
    11161 tacggtttcc gtgacgattg gcacgaccac ctataccacc gtgctggacg ccaacggcaa
    11221 ctggagcatc ggcgtgcctg ccagcgtcat ttccgcgctg gcgcaaggcg atgtgaccat
    11281 taccgccacg gttaccgact ccgccggcaa cagcggcaca gcctcgcaca ctgtcaccgt
    11341 ggcgctcggc gctccggtgc tcgccattaa caccattgcc gtcgatgaca tcatcaacgc
    11401 cacggagaaa ggcgcggatc tggcgatttc cggcaccagc gaccagcctg cgggcacgca
    11461 gattaccgtt acgctcaacg ggcaaaatta caccaccaca gccgatgctt ccgggatctg
    11521 gagcgtgacc gttccggcgt ctgcggttgg tacgcttggt gaagcgactt ataccgtgac
    11581 cgcctctgcc actgacatcg atggcaacag cggttccgcc agccataacg tgcaggttaa
    11641 taccgcgctg ccgggcgtca ccattaacgt ggtggcaacg gacgatatta ttaacgccgc
    11701 cgaagcgggc gcgggccaga ccatcagtgg gcaggtgacg ggtgctgcgg caggcgatac
    11761 cgttaccata acgctcggcg gagcgactta caccgctacg gtgcaggcga atttaagctg
    11821 gagcgtcgat gttccggcct ccgcgctaca ggcgttgggc aacggtgagc tgaccatttc
    11881 ggcttcggta acgaacagcg taggcaatac cggcaacggc acgcgtgaaa tcaccatcga
    11941 cgcgaatctg ccaggtctgc gggtcgatac cgtggcgggc gatgatgtgg tcaatatcat
    12001 cgagcacggg caggcgctgg tgattaccgg cagcagttcc ggtctagcga cgggcagcaa
    12061 cgtcacgctg accattaacg gacaaaccta tgttgcggcg gtactggcag atggcacctg
    12121 gagcgtcggc gttccggcgg cagatgtcag cgcctggcct gcgggattgg tgacgattac
    12181 ggcgagcggc aacacctccg ccggaaaccc ggtgagctta acgcatccgg tgacggtcga
    12241 tctctcggcg gtggcggtga gcatcaacgc tattaccgcc gatgatgtca tcaacgccgc
    12301 cgaaaaaggc gcggcgttaa cgctctctgg tagctcttcc ggcgtcgaag ccgggcaaac
    12361 ggtcaccgtg acctttggcg gcaaaaccta cacggcaagc gtcgcggcga acggttcctg
    12421 gagcaccacg gttccggcgg cagatatggc ggccctgcgt gatggcgatg ccagcgcaca
    12481 ggccagcgtc agcaatgtta acggcaacag cgccaccact acccacgctt acagcgtcga
    12541 tgccagcgcg ccaacggtga ccattaacac cattgcgggc gacgacattc ttaacgccgc
    12601 cgaagccgga gcggcgctga ccatcaccgg cagcagcacg gcggaagcgg ggcagacggt
    12661 gaccgtcacg ctcaatggcg caaactatat cggcacggta caaacggacg gcagctggag
    12721 cgtcagcgta ccgtcagccg acttaagcgc cctgaccgcc agcaactaca ccgtcagcgc
    12781 ggcggtgagc gacaaagccg gaaacccggc ctcggttaat cacaacctga cggtggatac
    12841 ttccgttccg gtcgtcacca tcaacacggt ggcaggcgat gatgtgatca acgcgacgga
    12901 acacgcccag gcgcagatca tcagcggctc cgccactgga gcggcaaccg gtagcaccgt
    12961 gacggtgact atcggcacaa atacctttac cacggtgctg gatgccagcg gcaactggag
    13021 cgtcggcgtt ccggcaagcg tcgtctcggc actggcgaat ggcacggtga ccatcaatgc
    13081 cagcgtcacc gatgccggag gaaacagcgg cagcgctacc catcaggtga cggtcaatac
    13141 cgggctgccg accattacct ttaacgccat cagcggcgat aacatcctga acgccgatga
    13201 aaaaggccag ccgttgacca tcagcggcgg cagtacgggg ctggcgacgg gcgcgcaggt
    13261 caccgtcacg ctcaacggcc acaactacag cgccaccacc gacgcatcgg gcaactggac
    13321 cttaaccgtg ccggtgagcg atctggcggc attaggtcag gccaactata cggtcagcgc
    13381 cagcgccacc agtgcagcag gcaacaccgc cagcagccag gcgaatttac tggtcgacag
    13441 cggcctgccg gacgtcacca tcaacaccgt ggcaggcgac gatattatca acgccgccga
    13501 agcgggggcc gatcaaacca tcagcggggt ggtgactcgc gccgccgctg gcgatacggt
    13561 caccgtgacg ctgggcggga acacttacac cgctacggta cagagcaact taagctggag
    13621 cgtcagcgtt ccgacagccg atctccaggc gttgggcaat ggtgatttga ccattaccgc
    13681 ctcggtcacc aacgctaatg gcaacaccgg gagcggcacg cgggatatca ccattgatgc
    13741 caacctgccg gggctgcgcg tagataccgt ggcgggcgat gatatcgtca acagcatcga
    13801 gcacgggcag gcgctggtga tcaccggcgg cagtagcggc ctgaatgcag gtgctgtgct
    13861 gacggttacc atcaacagtg tggcgtattc cgccacggtg caggcggacg gaagctggag
    13921 cgttggcatt ccggcggcaa acgtcagcgc ctggcctgcg gggccgttaa ccgttgaggt
    13981 agccgggcaa agcagcgccg ataacccagt cagcgtcagc catccgttca ccgtcgattt
    14041 aacggcggtg gcaatcagca tcaatacggt cgccagcgac gacgtgatta acgccgccga
    14101 gaaaggcacc aatctgaccc tttccggcag caccagcggg attgaaagcg ggcaaaccgt
    14161 aaccgtcacc tttggcggca aaacctacac cgcaagcgtc gccgccaacg gaagctggag
    14221 caaaacggtt ccggtggcag atctggcaac tctgccagac ggcgcggcga acgtgcaggc
    14281 cagcgttagc agcgcgagcg gtaacagtgc ctcggcgacc catgcgtata gcgttgacgc
    14341 cagcgcgccg acgctcacca ttaacaccat cgccagcgac gatatcctta acgccacgga
    14401 ggccggaagc ccgctcacca tcagcggctc cagcaccgcc gaaaccgggc agacggtcac
    14461 cgtcaccctt aacggcgcaa cctacaccgg cactgtacag gcggacggcg gctggagcgt
    14521 cagcgttcct acttcagccc tgggcgcgct caacgcaagc aattacaccg tcagcgccac
    14581 ggtcaacgac aaagcgggca accccggcag cgccagccac aatctggcgg tagataccac
    14641 cgcgccggtt ctcaccatta acaccgtggc gggcgatgac atcatcaacg acgccgaaca
    14701 cgcccaggcg ctggtgatct ccggtaccag cacgggcggg gaagcgggcg atgtggtgag
    14761 cgttgtgctc aatggcaaaa cctacaccac cacgctggat gcctccggta actggagcgt
    14821 cggcgtaccg gcggcggatg ttgcggcgct gggtagcggc gcacagacca tcaccgccag
    14881 cgtcagcgat cgggcaggca acagcgacga cgccagccgc accgtgaccg tcagcctcag
    14941 cgcgccagtg attagcatca acaccatcgc gggcgatgat gtgatcaacg cgacggaaaa
    15001 aggatctgat ctggcgcttt ctggcaccag cgatcagcct gcgggtacgg cgatcaccgt
    15061 cacgctgaac ggacaaaact acagcgccac taccgacgcc tccggcaact ggagcgttac
    15121 tgtgcccgcc tcggcagtca gtgcgctggg cgaagcgacc tacagcgtga cggcgagcgt
    15181 caccaatgct cagggtaaca gcagcaccgc cagccataac gtgcaggtta ataccgcgct
    15241 gccgggcatc accattaatc cggtggcgac ggacgatatt atcaacgctt cagaggcagg
    15301 cagcgcgcaa actatcagcg gccaggtcac cggggcggcg gcgggcagca ccgtcaccgt
    15361 tgaactgggc ggtaaaactt acaccgccac cgtgcagcca gatttaagct ggaatgtgag
    15421 cgtacctgcc gccgactggc aggcgctggg taacggtgag ttaacggtta atgcctcggt
    15481 gaccaacgcc gttggcaaca ccggtagcgg cacgcgcgat atcaccatcg atgccagtct
    15541 gcctggcctg cgggtggata ccgtcgcggg tgatgatgtg gtcaatatca tcgaacacgc
    15601 tcaggcgcag gtgatcaccg gcagcagctc cggctttgcc gcaggcacgg cgttgacggt
    15661 ggtgattaac aaccaaactt acgccgcgac ggtgctggcg aacggcagct ggagcgtcgg
    15721 cgttccggcg gcggatgtca gtaactggcc tgcgggaacg ctgaatatta ccgttagcgg
    15781 cgcgaacagt gccggaacgc aaaccagcat tacccatccg ctgactgttg atctcactac
    15841 cgttgccatc agcattaacg ctattacccc tgatgatgtg attaacgccg ccgaaaaagg
    15901 cgcggcgcta atgctctccg gcagcacgtc cggcgtcgaa gcggggcaaa ccgtcaccgt
    15961 caactttggc ggcaaaacct acacctccac ggtggcggct aacggtagct ggagcaccac
    16021 cgttccggct gcggatttgg cggcgctgcg tgacggcgat gccagcgccc aggtgcgggt
    16081 gactaacgtc aacggcaaca gcgccacagc cacgcatgaa tacagcgtcg atagcgccgc
    16141 gccaacggtc accatcaaca ccattgccag cgataatatc atcaacgcca gcgaagcggc
    16201 ggcgggcgta acggtgtccg gcaccagcac ggcgcaaacc gggcagacgc tcaccgtcac
    16261 gcttaacggc actaactacc agaccacggt gcaggcagac ggcagctgga gtttaacgct
    16321 gcccgccagc gacttaacag cgcttgccaa taacggctac accctgaccg ccacggtcag
    16381 cgatctggcg ggtaaccctg gcagcgccag caaaggcgtg accgtcgata ccactgcgcc
    16441 ggtgatcagt tttaacaccg tggcgggcga tgatgtgatt aacaacgtcg aacacactca
    16501 ggcgcagatt atcagcggca ccgccacggg cgcggtggcg ggcgaccgcc tggtggtgac
    16561 catcgccggg cagcagtatg tcaccagcac cgatgccagc ggcaactgga gcgtcggcgt
    16621 gcctgccagc gtgatttccg gccttgcgga tggcacggtg accatcagcg ccaccattac
    16681 cgatagcgca ggcaacagca gcacgcagac gcacaacgta caggtgaaca cggcggcagt
    16741 gtcgctttcg gtcagcacca tcagcggcga taatattatt aacgccgccg aagcgggcag
    16801 tgcgctgacc ctgagcggca ctggcactaa tttcgcgaca ggtacagtgg tgaccgtgtt
    16861 gcttaacggc aaaggctaca gcgccaccat tcagagcaac gggagctgga gcgtgaacgt
    16921 gcctgcggcg gatgttgcgg cgctcagtga tggcaccagc tacacggtta gcgcctccgc
    16981 tcaggacagt gccggaaaca gcgccacggc ttcgcgcagc gtggcggtgg atctcaccgc
    17041 gccggtgatc agcattaaca ccgtttcgac ggatgaccga ctcaacgccg ccgaacaaca
    17101 gcagccgtta acccttaacg gctcgaccag cgcggaagtg ggacagacgg tcaccgtcac
    17161 ctttggcggc aaaacctata ccgccacggt tgctgccaat ggcacctggg cgctgaacgt
    17221 gcctgcggca gatctggccg cgctcgggca gggcgcgcag accattaccg ccagcgtgaa
    17281 cgatcgcgcc ggtaaccccg gacaggccac gcacgccctg accgtcgata ccgttgcgcc
    17341 aacggtcacc atcgccacgg tggcgggtga cgatattatc aacaacgccg agcagcttgc
    17401 cgggcagacc atcagcggca ccaccaccgc cgaagtaggc cagacggtga ccgtcacttt
    17461 taatggtcag agctggagcg caacggtcgg cagcggcgga agctggtcgg tatttattcc
    17521 ggcgcagcag tttgccggat taagcgacgg cagctacacc atcagcgcga cggttagcga
    17581 tcaggccgga aaccccggca gcgccagccg tggcgtgacg ctgaacggcg acgtgcccac
    17641 cgtcaccatc aacacctttg cgggcgacga tgtggtgaat gcagcggaac acggctcatc
    17701 gctggtgatc agcggcacca ccaccgcgcc cgtcgggcag acgctgaccc tgaccttaaa
    17761 cggcaaaacc tacaccacca cggtgcagac gggcggtagc tggagctaca ccctcggcag
    17821 cgccgatgtc accgcgctgg cagacggcaa cgcctacgtg attaacgcct cggtgagcaa
    17881 cgctatcggc aacaccggta gcagcaatca caccattacc gtcgatctca gcgccccggc
    17941 gatgggtatt aatatcgatt ccctgcaagc cgacactggc cttagcgcca gcgactttat
    18001 caccagcgtc agtccggtgg tggtcaacgg ctcgctcacc gccgcgcttg ccagtaacga
    18061 gacggcgcaa ataagtatcg acggcggcac aacctggacc acgcttaccg ttaccggcac
    18121 gacctggcgc tataacgaca gccgcacgct gaccgatggt aactatctct atcaggtgcg
    18181 ggtgattgac gcggcgggca acgtcggcgc gactgacagc cagaacgtgg tgatcgacac
    18241 catcgcgcca gatccggcag tgaaaaccat cgccatcagc gcgatcacca ccgataccgg
    18301 cttgatcact aacgattttg ttaccagcga cacgacgctt gcggtgagcg gcacgctggg
    18361 cgcggcgctt tctgtcggtg agttcgcgca aatcagcatt gatggcggca ccacctggca
    18421 gaatctttcg gtaagcggtt tgacctggac atatctggac gggcggacgc tgagcgacgg
    18481 taattacaac tatcaggtgc gggtgatcga caccgccgga aatatcggcg ccaccgccag
    18541 ccagatagtc accgtggata ccaccgcgcc gctggcgagc aaaaccatcg ccattgcggg
    18601 catcagcgat gataccgggt taagcagcag cgattttgtt actcgcgaca ccacgctgac
    18661 cgtgcgcggc acgctcggcg cggcgctggc ggccgatgaa cgggcgcaaa tcagcctcga
    18721 cggcggcgtc acctggacta cgttaaccgt tatcggcacc agctggagct atgccgatgg
    18781 tcgcacactg accgacggta cctggaacta cacggtgcgg gtggtggatc tggcggggaa
    18841 tgtcgggcag accgcgacgc aaaacgtggt ggtcgacacc accagcccgg aagcggcgaa
    18901 aagtatcacc attaccggta tcagcgatga caccggaacc agcagcactg attttattac
    18961 cagcgacacc tcgcttaccg tgcgcggcgt attgggcgcg gcgctcggcg ctaacgagtt
    19021 cgcgcaaatc agcaccgaca acggcgcaac ctgggtgaac gtgaccgttg ccgcagacgg
    19081 cctgaactgg agttacgttg acgggcgcat gctcaccaac ggcaccacca cctggcaggt
    19141 gcgggtggtc gatctggcgg gcaacgtcgg tgcaaccggc agccagtcgg cgcagattga
    19201 caccgttaac ccggcgcagg tgctcaccat cgccagtatc agcaccgaca cggggagttc
    19261 ggcaactgac tttatcacca gcgacaccac gctcacgctg acaggttcgc tgggagcggg
    19321 gcttgccagc ggcgaagtgg cgcagattag ccttgatggc ggcgcgacct ggacaacgct
    19381 taccaccaac ggtacacagt ggacttacac cgacagccgc acgctgaccg acggcagcta
    19441 cgtttatcag gtgcgggtgc tggatctggc gggtaacacc ggcccggtgg tgtcgaaaac
    19501 ggtggtggtc gatacgatta accccaccgc cacgccgacc attgtgtcgt ataccgatga
    19561 tgtcgggcag cggcagggga cgttaaacag ttcgcaggcc accgacgaca ccacgccgct
    19621 gctgaacggc gtgctctctg cgccgcttgc cagtggtgaa gtggtttacc tctaccgtaa
    19681 cgggctgctg ttaggcgcgg tgacgatggt cggcgcactg aactggacct acagcgatag
    19741 cggtctggtc agcggggcct acacctacag tgcgcgagtg gtggatttgg cggggaatat
    19801 cacctcctcc agtgattttg tcctgacggt cgatacctct attccgacca cgctggcgca
    19861 aatcaccagc cagaccacgc gcgatactac gccgattatc agcggggtga tcaccgccgc
    19921 gcttgccagt gggcagtatg ttgaagtggt gatcaacggc aaaacctaca cctctgaacc
    19981 gggcggcgcg gtagtggtcg atccggcgca caacacctgg tatgtacagt tgccggatac
    20041 cgatgcgctg acagtttccg cgaccgccta taccgttact gcacaggtaa aaagttcggc
    20101 gggtaacggc aataacgcca atattagcaa cggcacggtg acggttaacg cggcgattga
    20161 ttacacaccg acctggacca ccgccagcaa aaccaccgcc tgggggctga cctacggcct
    20221 cgactcgcac gggatgtgga cggtgctggc aaaccagcag gtaatgcaat cgactgaccc
    20281 actcacctgg tcgaagaccg cgctgacgct gtatcagagc ggcaacaact acgccaccag
    20341 ctccatcgcc gattacgacc gtaacggcac gggcgatctg tttatcaccc gtgatgacta
    20401 cggtacaggc tatatcaacg gctttaccaa taacggcgac ggcacctttt ccagcgctat
    20461 tcaggtcacc gtcggcaccc tgacgtggta cggctcgatt gtggcatttg ataaagaggg
    20521 cgacggctat ctcgacttct ggattggcga cgcgggcggg ccggactcca acaccttcct
    20581 gtggaacaac gcaggtacgc tggtaggtaa ctccaccacg tcgaacagcg gcggtagcgc
    20641 cacggtgggc ggggcggtga cggggtatct ttcgctcaac gaaggttctg gcgtcgatct
    20701 gaacaatgac ggcaggatcg acctggttca gcacacctac aacctgaaca actattacac
    20761 gctatcttcg ctcatcaacc aggggaatgg gacgtttgtc tgggggcaga acaccaccaa
    20821 taccttcctg agcggggcgg gtagtggcgc tatgagcagc agcgtttcca tgacctgggc
    20881 cgatttcgat ggtgacggcg atatggatct cttcctgccc gccagccagg gaagagctaa
    20941 ctacggctcg ctgttattca acaccaacgg cgtactgggt agcccggtgg cggtgggcgc
    21001 aacggcaacc acctacgcca gccagtttag cctggcggtg gactggaacc atgacggcct
    21061 gatggatatc gcccgtatcg cccagactgg acaatcgtat ctttacacta acgttggtgg
    21121 tgccagcaac tggacacaat cagccctcgg cggcagccag agcggtacca ccagcggcgt
    21181 ggcggcaatg gactacgact gggacggcgc ggtggatgtg ctggtgacca aacagtcggg
    21241 cagcgtgttc ctgatccgca acaccaacac ggtgagctac ggcacttcgc tacacctgcg
    21301 catcaccgat cccaacggca ttaacgtcta ttacggcaat accgtgaagc tgtacaactc
    21361 ggcgggagtg ctggtcgcca cgcaaatcat caacccgcag tcgggtatgg gggttaacga
    21421 cacctcggcg ctggtcaact tctacgggct gaatgccgga gaaacctaca acgcggtgct
    21481 gatcaaatcc accggcacca ccgccagcaa tatcgaccag acggtcaaca ccagctgggg
    21541 cggtttacag gccaccgatg ccactcacgc ttacgacctc agcgccgaag cgggtaccgc
    21601 gagcaacaac ggcaagttcg tcggcaccgg ttataacgac accttctttg ccaccgcagg
    21661 caccgatacc tacgacggtt ccggcggctg ggtgtacagc tccggcaccg gaacgtggct
    21721 ggcgaacggc gggatggacg tggttgattt ccggctttcg acggtgggcg tgacggctaa
    21781 cttaagcagc accgccgcgc aggccaccgg ttttaacacc tcgacattta ccaatatcga
    21841 aggcatttcc ggatcgaatt ttaacgacac tctgaccggc agcagcggcg ataaccaact
    21901 ggaagggcgc ggcggcaacg acacgctcaa catcggcaac ggcggccacg acaccttgct
    21961 ctataaactg ctcaacgcca gcgacgccac tggcggcaac ggttcggatg tggtgaatgg
    22021 ctttacggta ggcacctggg aaggcactgc cgacaccgat cgcattgata tccgcgagtt
    22081 gttgcagggc agcggctaca ccggaaacgg caaagccagc tatgtcaacg gcgtggcgac
    22141 gctggatgcg caggccggaa acatcggtga ctttgtcaaa gtcacccaga gcggcagcga
    22201 caccatcgtg cagatcgacc gcgacggcac gggcggcact tttgcgacaa ctaacgtggt
    22261 cacgctgacg ggcgtgcaca ccgacctcgc cactttgctg gcgaatcatc agttgatggt
    22321 ggtgtagata atgaaaaaaa atgcccagtc cgtggaagcc tggctggagg cgatgatcgc
    22381 cgtggcgcgc tattatcgcc ttgatttttc tcaggagaat gtgcgcgcaa ccgtcaactg
    22441 ggagcgcgac agcaagcggg aagagttact caccgacatg gcgcggcagt tgggaatggg
    22501 gttacggttg gtggagtttt ccgccgattc gcttaacccc tggcgtctgc cgctgatcgc
    22561 ggtgtttgat aatcagcaga ttggcgtgat cacccgtcgc gacaatcatg acaatatcag
    22621 cgttcagttc agcggtgacg aagggctgga aaccacgctt aacgtcgcgg atgtcgagga
    22681 taaaatcgtt gaactggcgc tactgcgccc actcagcgcc atccctgacg cgcgggtgga
    22741 tgactatatt cgcccgtatc aggcgaactg gttctggaac ctctcgctaa aagactggcg
    22801 gcgctatggc gatattatgc tggcgtcact ggtggcgaac gtgctggcgc tggcagcgat
    22861 gattttctcg atgcaggttt acgatcgggt ggttcctgcg cagtcctatc cgacgctatg
    22921 ggtgcttttc gccggggtga tgatggcgat cctgtttgag ttctgtatgc gcatggtgcg
    22981 cacgcatctt tctgatgtga tcggcaagcg ggcggatctg cgtatttcgg atcgcgtttt
    23041 tggtcacgca ttacggctga aaaacaacgt gcgatcgaaa tccaccggat cgtttatctc
    23101 gcagatccgc gaactggaat cagtgcggga gcttattacg tccaccacca tcggcgcggt
    23161 ggcggacctg ccattcttcc tgctgtttgt ctttattttg tggatgatcg gcggctggct
    23221 ggtgctggtg gttttgctgg cgctgccgtt gctggtgatc cccggcctgc tggtgcaacg
    23281 cccgctggcg cggctggcga acgaagggat gcgcgagtca gcggtacgca acgctacgct
    23341 ggtggaagcc gtgcagtcga ttgaagatat taaactgctt cgcgctgaac agcgttttca
    23401 gaatcagtgg aatcacacca acgacgtggc ctcgtccatc agcatgaagc aacgttttct
    23461 caccgggcta ctgctcacct ggacccagga agtgcaatcc atcgtctacg tcgtggtgct
    23521 gttggtgggc tgctttatgg tgatgaacgg cgacatgacc accggtgcgc tggtcgggac
    23581 gacgatcctg gcttcgcgta ccattgcgcc gctgtcgcag atatccggcg tgctctcgcg
    23641 ttggcagcag gcaaaagtgg cgcgtaacgg ccttgatgaa ctgatgaagc gcccggtgga
    23701 tcagccggaa cacggcaaac tggtgcataa agcggtgctg cacggtaatt atcagttcag
    23761 caacgcggtg ttttattacg acgaagaaga gaaaattgcc gatgtggcga tcggcaaact
    23821 caacattcag gctggtgaga aaatcgccat tctcgggcgc aacggcgcgg gtaaatcgac
    23881 gctactgcaa atgctcgccg gaatgcgtat cgcccagcag gggcaggtgt tgctggataa
    23941 catcagtatc gggcagcttg atccggcaga tctgcggcga gacatggggc tactcagcca
    24001 gaccgggcga ctgttctttg gttcgctgcg ggaaaacctc accatgggga tgccggaagc
    24061 cagcgatgaa gatatcgaac gcgcgttaac cctcagcgga gcgctgccct ttgtgcagaa
    24121 gcagaaaaac ggcctcaact acatgattca ggaaggggga tttggcctct ctggcggcca
    24181 gcggcaaacg ctgctgctgg cgcggctact catctctcaa cccaatatcg tcctgctcga
    24241 cgaacccagc gcctcgctgg acgaaatggc agaagcgcat ttaatcgaac aactgaaaca
    24301 gtggattggg catcgcacgc tggtcatcgc cactcaccgc acggcgatgc tgcaactggt
    24361 ggaccgcatt atcgtgatgg atcaggggcg gattgtgatg gatggcgcga aagaggccat
    24421 tttgcgtgaa cagggcgaac ccacgacgcg acgggtggtg ttgcaggaga agaataaggg
    24481 gtctgccgca tgaacggaca actgcaatat aaccgtggcc tgactgaacc ccgcttgccg
    24541 cgttcggcgc tggcggtacg cgttaccgct gtaatgctgc tctgttttct cggctgggcg
    24601 tggtacttcc agcttgatga agtcaccacc ggcagcggca cggtagaacc ctccgggcgc
    24661 gaacaggtgg tgcagtcgct ggaaggcgga attctgtatc atctgaacgt gaaagttggc
    24721 gatattgtcg aacagggcca gccgctggcg cagcttaacc gcactaaaac ggaatccgac
    24781 gtccaggaag cgatgtcgcg gctgtacgct gcgctggcaa cctcggcgcg gctgcgggcg
    24841 gaagtgagca ataaaccgtt ggtcttccct gacgaattga ataaattccc cgaacttatc
    24901 gagtcggaaa cggcgttgta caacactcgc cgcgacgggt tgaacaaagc caccaccggg
    24961 ctgacgcagg ggattagcct ggtcaaccgt gaactggcga tgacccagcc gctggtgaaa
    25021 cagggggcgg cgagcagcgt cgaagtgctg cgcctgcaac gccaggccaa tgaactggaa
    25081 aacaaactca gcgacgtgcg cacgcaatac tacgttcagg cgcgcgaaga gttagcaaaa
    25141 gccaatgcgg aagtggaaac ccagcgttcg gtgatccgtg ggcgtgaaga ctccctcacc
    25201 aggctcaact ttactgcgcc agttcgtggc attgttcagg atattgacgt caccacagtt
    25261 ggcggcgtca tcgcccccgg tggtaaactg atgaccatcg tgccgctgga tgagcaactg
    25321 ctgattgagg caaaaatctc cccgcgcgat gtggcgttta tccatccggg gcaaaaatcg
    25381 ctggtgaaaa taactgccta cgattactcc atctacggcg gcctgccggg agaagtagcg
    25441 gtgatatcac cagacaccgt gcaggatgaa gtgcggcgcg atgtttatta ctatcgggtg
    25501 tacattcgca ctttcagcaa ccatctggaa aacaaaagca aacagcagtt tccgatattc
    25561 cccggtatgg tggcgacggt cgatatccgc accggcaaaa aaagcgtgct ggattatctg
    25621 ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac gatgaacatt agcgatgtag
    25681 caaaaattac cggcctgacc agcaaagcca ttcgcttcta tgaagagaag gggctggtga
    25741 cgccgccgat gcgcagcgaa aatggttatc gaacctacac gcagcagcat ctcaacgaat
    25801 tgaccttact gcgccaggcg cgccaggtgg gctttaacct ggaagagagc ggcgagctgg
    25861 tgaatctgtt taacgacccg cagcgtcaca gtgccgacgt caaacgtcgc acgctggaga
    25921 aggtggcgga gattgaacgg catatcgaag aactgcaatc tatgcgcaac cagctactgg
    25981 cgctggcgaa tgcctgcccc ggtgatgaca gcgccgactg cccgattatc gaaaatctct
    26041 ccggctgctg tcatcatcgg gcggggtgat taacgcttgc gaaaatagtc gtccattatt
    26101 ttcgtcaact ccggacgttc gccgtcatca ttgatggcaa aatccagaaa aaaagcatct
    26161 gcttgcggcg ttcggttttg cgccatcaac cggaaggtga gggggcgata actgacgtat
    26221 gccggatagc tgataacgaa tcgggcaaag tcaataaaaa gcggcgagtg tgtgaaaaac
    26281 agcgcctgca aaattacatc agccttttca tcctccccca aaccttgttg gatcatgctc
    26341 ttaatacgag tttcgctcaa attcccggcc tcaacaattt gccaggcttg atccagtgaa
    26401 gggtaccggc tgacgctctg ctgcaactct tctgaccagg tttcggatgg gctgatgtgc
    26461 ttatcagggc cgaaactctc ccatgcgccg gacggtaatg ttttccactc agtgagtttg
    26521 ggaacgtggc acagtagctc tgttttgtgc agacattctc cgcccggaaa taccagggtt
    26581 tgttgctcat cattaaaacg ggttgccgtg ctgagtacca gtgttttgag cggtcgccct
    26641 tccagtgcgg cagcagtggc cttatggtgc ccgtcgagca gaacgcagag ataggaatca
    26701 gggagatacc aggccaaagc gcgggcgtcc ggtttatcgc gataaaacgt cacacgctca
    26761 ggattaaaat gtgatggcgg ctgtccagga agaagaaaac agggggcggt gttacagaaa
    26821 tagcaatatt caggatccca gacggcaacg gttgccggat tactggcgct atggtccgtt
    26881 gccgcccaaa agaaatgccc attgccatcc gtcgggtaaa gttcacgatc ttccagcaga
    26941 taatagccat cagcaaggca tgagaacagc ggtgtaagcg cggcaaaagc gtccgtaaag
    27001 ccagaccatg ggcgattcca gttttcgaga gcagacaaaa accgggcggc tccctgttca
    27061 tcatccgcaa aaccgctgta gacaaattcc gcgcaggtgg ggcagccggc ttcggaccag
    27121 aaacgtatta ccgggacatc ctgccagcag agtgaacgga taagcccata agggtgcgga
    27181 tcgtaaagca cacattgacg cagaaatcct ttaccggact tcaccgaaaa aatgacgccc
    27241 gcagcggtat ttttcagcag acgtacttca ggttttcgaa agggaaaaag catggccact
    27301 ccttagccga atattcagaa ccctgaactg tgccagaaaa ttacgctggt gactattgct
    27361 ctggaaatgg taacggtatc cttacccatt aatccccgtt tgcaccgaaa gaatatgctg
    27421 caaactttct accattatgc gccccgattg aaataagcct tcctggattt cattgttaac
    27481 attcacagcg tgaaccaggg aggctaataa caccacttgc aagaaggcaa aaataaacgc
    27541 accgataatc ataatatgcg tcaggggtat tttcctttgc tccttcattt taaatagccc
    27601 acaaactggc cttctttcat taaggcgatt tttcctgtgt gatgataaac aatgacgtta
    27661 tacagacccg actgataaac cgtggcatcg gcattatcct catctatggc acataatgaa
    27721 tcattgagat aagcaaagtt cttactgctc attctgaagg tgacgccaaa tcctttataa
    27781 atggctttct ttgccgcctg ggcgtgttgt gcggcttctg catttttaaa tgcctgtgcc
    27841 tcggaagcac ttaaaatagc cgtctggcgc tgctgttctg cgagataaca ggtgtcatgg
    27901 ctgacgccct gcgcctggca atctgcgatg cgggaagtgg gtgaactaca gccagccagt
    27961 aatataaccg ccagcaggga aaaggattta atggagctat tcatcattgt taaccttaaa
    28021 taatgtacaa attaatgaag aggtgaaaac cggcaagctt ccttgctctt atttatattg
    28081 aggattgaaa tattagaacg aatatcccac gccgacaccc gcgccgaaat cagattgtgt
    28141 atccgcagag acaccggctt taatcacggt atgcgttccg gcattaaaag atgttcctac
    28201 agacaccgca gattcaccgt tgaaggttcc cgctcccgct gacaccatca tcaagtcacc
    28261 ggttttcacc tgtggttgtg aggcgatagc gatggcagat gcagttcccg cattggcttc
    28321 tttacgatta tcctcaactt cgtttttcag cgaactgaaa tggttattga gcgtagtaat
    28381 ctgttggcta ttatccgcta ctcgtttttg cgtgttgctt aactgctgat gcgtttgggt
    28441 tacgtcttgt gtcaggctgt caatttgttc atgcgtttgc gttgagtctt gcgccagacg
    28501 ctgaatttgt tccccgtact gcacactatc gatatgctgt tgcgttgcct ggttagtcgc
    28561 cagagtatct attttttgtt tttgttctgc tgcagtacgc gcattcgcat cattttgtgc
    28621 tttcagtccg gcgttaacca tctcttgata atgtgcgttg gtctgggcat cgccaagtgc
    28681 ggaaaccgtt ttttgcgtcg cgtcgatttg tttctgctga tcgtccattt gttgctgctg
    28741 ggtgtccgct tgttcctgtt gcgcattggc agcggctgtc tgtctggcgt caatcatgct
    28801 ctgatatcgg gcggcagtct gcacatcccc ggtggcggca acggatttcc gggtttcgtc
    28861 gagttgttgc tgttggtcag caatatcgct gcggttctgt tcgatattat ttttgttttc
    28921 tgtcacagca ccggaaagtt tgacgccgtt aatcagagcg gtgttggcgt tagcgttagc
    28981 atggtcagcc gttgactggg catgtgtgac atcgcctttt atgttggtga cgtctgactg
    29041 cactgcggag acatcattac gggtgtcggc gatggcggct ttattggcat caatatccgt
    29101 ctgcgccacc gtgatggcgt gctgcgcgtc atcagctttc agtgaagccc agttggcggc
    29161 atgttgtgcg tcctgaatgg cggtgtcctg ctcgttgtcg cggacggtct gtttgtgctc
    29221 gtttgatgcc agccgtttct gctggtcagc aatatcgctg cggttctgtt cgatattgtt
    29281 tttgttttct gtcacagcac cggaaagttt gacgccgttc atcagagcgg tgttggcgtt
    29341 agcgttggca tggtcagccg ttgactgagc atgtgcgaca tcggctttta tgttggtgac
    29401 gtctgactgc actgcggaga catcattgcg ggtgtcggtg atggcggctt tattggcatc
    29461 aatatccgtc tgcgccacca taatggcatg gtgcgcatca tcagcttttt gcgacgccca
    29521 gttggcggca tgttgcgcgt cctgaatggc ggtgtcctgc ccgttgtcgc ggacggtctg
    29581 cgcttgattg gttatcgggt tcagcgtact ggcgggctgt acgggagaag cagttgtatt
    29641 ttcctgaggc gtcgttgtct ggtgatcatg cgcaatcaga taattttgtg cggctgcgct
    29701 atttcctgtc ctttctaaaa atcgaccaat tttctgatca atttccactt gcgacattcc
    29761 tggctggatt gccgctaatt ctgtcgctgc cgcctcaaca gtatcacccg caaacgcaga
    29821 tggacttact gcggtagcaa caacgacagc cagtaaagac aactttacag tattcatgta
    29881 ttttttctcg tccaaagtaa gtcacatatt tgtttctata agcaacataa aacgttgtta
    29941 taagaattaa tctgcgttgc aatttaatcg aaatctacgc cgccatttgt gtgatgtaaa
    30001 gcgcattttt gtctggt
//
LOCUS       DAZFFG010000049        29854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFFG010000049 DAZFFG010000000
VERSION     DAZFFG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.049"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(238..2067)
                     /gene="glmS"
                     /locus_tag="ACHJ7J_002490"
     CDS             complement(238..2067)
                     /gene="glmS"
                     /locus_tag="ACHJ7J_002490"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO5000711.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2229..3599)
                     /gene="glmU"
                     /locus_tag="ACHJ7J_002491"
     CDS             complement(2229..3599)
                     /gene="glmU"
                     /locus_tag="ACHJ7J_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO5000712.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3951..4370)
                     /gene="atpC"
                     /locus_tag="ACHJ7J_002492"
     CDS             complement(3951..4370)
                     /gene="atpC"
                     /locus_tag="ACHJ7J_002492"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO5000713.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4391..5773)
                     /gene="atpD"
                     /locus_tag="ACHJ7J_002493"
     CDS             complement(4391..5773)
                     /gene="atpD"
                     /locus_tag="ACHJ7J_002493"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO5000714.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5800..6663)
                     /gene="atpG"
                     /locus_tag="ACHJ7J_002494"
     CDS             complement(5800..6663)
                     /gene="atpG"
                     /locus_tag="ACHJ7J_002494"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO5000715.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6714..8255)
                     /gene="atpA"
                     /locus_tag="ACHJ7J_002495"
     CDS             complement(6714..8255)
                     /gene="atpA"
                     /locus_tag="ACHJ7J_002495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO5000716.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8268..8801)
                     /gene="atpH"
                     /locus_tag="ACHJ7J_002496"
     CDS             complement(8268..8801)
                     /gene="atpH"
                     /locus_tag="ACHJ7J_002496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO5000717.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLKALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8816..9286)
                     /gene="atpF"
                     /locus_tag="ACHJ7J_002497"
     CDS             complement(8816..9286)
                     /gene="atpF"
                     /locus_tag="ACHJ7J_002497"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO5000718.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9348..9587)
                     /gene="atpE"
                     /locus_tag="ACHJ7J_002498"
     CDS             complement(9348..9587)
                     /gene="atpE"
                     /locus_tag="ACHJ7J_002498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO5000719.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9634..10449)
                     /gene="atpB"
                     /locus_tag="ACHJ7J_002499"
     CDS             complement(9634..10449)
                     /gene="atpB"
                     /locus_tag="ACHJ7J_002499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO5000720.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10458..10838)
                     /gene="atpI"
                     /locus_tag="ACHJ7J_002500"
     CDS             complement(10458..10838)
                     /gene="atpI"
                     /locus_tag="ACHJ7J_002500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO5000721.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11455..12078)
                     /gene="rsmG"
                     /locus_tag="ACHJ7J_002501"
     CDS             complement(11455..12078)
                     /gene="rsmG"
                     /locus_tag="ACHJ7J_002501"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO5000722.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12142..14031)
                     /gene="mnmG"
                     /locus_tag="ACHJ7J_002502"
     CDS             complement(12142..14031)
                     /gene="mnmG"
                     /locus_tag="ACHJ7J_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO5000723.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14410..14853)
                     /gene="mioC"
                     /locus_tag="ACHJ7J_002503"
     CDS             complement(14410..14853)
                     /gene="mioC"
                     /locus_tag="ACHJ7J_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO5000724.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14943..15401)
                     /gene="asnC"
                     /locus_tag="ACHJ7J_002504"
     CDS             complement(14943..15401)
                     /gene="asnC"
                     /locus_tag="ACHJ7J_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO5000725.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15553..16545
                     /gene="asnA"
                     /locus_tag="ACHJ7J_002505"
     CDS             15553..16545
                     /gene="asnA"
                     /locus_tag="ACHJ7J_002505"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO5000726.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16550..18001)
                     /gene="viaA"
                     /locus_tag="ACHJ7J_002506"
     CDS             complement(16550..18001)
                     /gene="viaA"
                     /locus_tag="ACHJ7J_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO5000727.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRNRLKDARVPQELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSAITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17995..19491)
                     /gene="ravA"
                     /locus_tag="ACHJ7J_002507"
     CDS             complement(17995..19491)
                     /gene="ravA"
                     /locus_tag="ACHJ7J_002507"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO5000728.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19714..21582
                     /gene="kup"
                     /locus_tag="ACHJ7J_002508"
     CDS             19714..21582
                     /gene="kup"
                     /locus_tag="ACHJ7J_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO5000729.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21749..22168
                     /gene="rbsD"
                     /locus_tag="ACHJ7J_002509"
     CDS             21749..22168
                     /gene="rbsD"
                     /locus_tag="ACHJ7J_002509"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO5000730.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22176..23681
                     /gene="rbsA"
                     /locus_tag="ACHJ7J_002510"
     CDS             22176..23681
                     /gene="rbsA"
                     /locus_tag="ACHJ7J_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO5000731.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23686..24651
                     /gene="rbsC"
                     /locus_tag="ACHJ7J_002511"
     CDS             23686..24651
                     /gene="rbsC"
                     /locus_tag="ACHJ7J_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5000732.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24676..25566
                     /gene="rbsB"
                     /locus_tag="ACHJ7J_002512"
     CDS             24676..25566
                     /gene="rbsB"
                     /locus_tag="ACHJ7J_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO5000733.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25692..26621
                     /gene="rbsK"
                     /locus_tag="ACHJ7J_002513"
     CDS             25692..26621
                     /gene="rbsK"
                     /locus_tag="ACHJ7J_002513"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5000734.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANISFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLKSVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26625..27617
                     /gene="rbsR"
                     /locus_tag="ACHJ7J_002514"
     CDS             26625..27617
                     /gene="rbsR"
                     /locus_tag="ACHJ7J_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO5000735.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRIIQPTLQQQRLQLTPILMERGSA"
     gene            complement(27583..29010)
                     /gene="mdtD"
                     /locus_tag="ACHJ7J_002515"
     CDS             complement(27583..29010)
                     /gene="mdtD"
                     /locus_tag="ACHJ7J_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO5000736.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKIRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(29033..29725)
                     /gene="yieP"
                     /locus_tag="ACHJ7J_002516"
     CDS             complement(29033..29725)
                     /gene="yieP"
                     /locus_tag="ACHJ7J_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO5000737.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VINHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7191 a         7623 c         7629 g         7411 t
ORIGIN      
        1 ttgttttaca agcgattctt gaatgaacaa aacgtgctat tcattacaaa gtacgtcaat
       61 tatggcaatt gtttgtcaat gattcgaggt atttaattac tactacggtt aaaacaagtg
      121 ttcaacggtg ccccaaataa tagctgaagt taattatatg gaatgaggtt agattattaa
      181 tgctggtaag taaaagggcc gccaaaaggc agcccttcgt tcagcaaatg aataatatta
      241 ctcaaccgtt accgattttg ccaggttacg cggctggtca acgtcggtgc ctttgatcag
      301 cgcgacgtga taagccagta actgcagcgg aacggtgtag aagatcggtg caatcacctc
      361 ttccacatgc ggcatctcga tgatgtgcat gttatcgcta cttacaaaac ccgcatcctg
      421 atcggcgaag acatacaatt ggccgccacg cgcgcgaact tcttcaatgt tggatttcag
      481 tttttccagc aattcgttgt tcggcgcaac aacgataacc ggcatatcgg cgtcaatcag
      541 cgccagcgga ccgtgtttca gttcacctgc agcgtaggct tcagcgtgaa tgtaagagat
      601 ctctttcagc ttcaatgcgc cttccagcgc gattgggtac tgatcgccac ggcccaggaa
      661 cagcgcgtga tgtttgtcag agaaatcttc tgccagtgct tcaatgcgtt tgtcctgaga
      721 cagcatctgc tcaatacggc tcggcagcgc ctgcagacca tgcacgatgt catgttcaat
      781 ggaggcatcc agacctttca ggcgagatag cttcgccacc agcattagca gcacggtgag
      841 ctgggtggtg aatgctttag tggatgccac gccgatttct gtacccgcgt tggtcattag
      901 cgccagatcg gattcgcgca ccagagaaga acccggaacg ttacagattg ccagcgaacc
      961 aaggtaaccc agctctttcg acagacgcag gcccgccagg gtatccgcgg tttcgccaga
     1021 ctgtgacaag gtgatcatca ggctgttacg acgcacggca gatttacgat agcggaattc
     1081 agaggcgatt tcgacgtcgc acggaatacc tgccagcgat tcaaaccagt aacgggaaac
     1141 cataccggag ttataagaag taccacaggc gaggatctga atatgctcaa ccttcgacag
     1201 cagttcgtcg gcgttcggtc ccagctcgct taaatcaacc tgaccgtggc tgatgcgtcc
     1261 ggtaagggtg tttttgatcg cgttcggctg ttcgtagatc tctttctgca tgtagtgacg
     1321 gtaaatacct ttatcgcccg cgtcatattg cagattggat tcgatatcct gacgttttac
     1381 ttccgcgcca gttttatcga agatgtttac cgaacggcga gtgatttccg caatatcgcc
     1441 ctcttcaagg aagataaagc gacgggtcac cggcagcagc gccagctggt cagaagcgat
     1501 aaagttttca cccataccca gaccaatcac cagcggacta ccagaacgtg ctgccagcag
     1561 ggtatccggg tgacgggagt ccatgatcac tgtaccgtac gcaccacgca gttgcgggat
     1621 agcacgcaga acagcctcac gcagagtccc gccttgtttc agctcccagt tcaccagatg
     1681 ggcaatcact tcggtgtcgg tttcagaaac gaaggtatag ccacgcgctt ttaactcttc
     1741 gcgcagcggt tcatggtttt cgatgatgcc gttatgcacc accacaatgt gttcagaaac
     1801 atgcggatgc gcattcactt ctgaaggttc accgtgggtc gcccagcgag tgtgagcaat
     1861 accggtgccg ccatgcagag gatgttcttc cgctgcctga gccagcatct ggactttacc
     1921 gaggcgacgc aggcgggtca tatgaccttc tgcatcaaca acggccagac cggcagagtc
     1981 atatccgcgg tattccagac gacgtaaacc ttcaagaagg atttctgcta catcacgttg
     2041 cgcgatcgcg ccaacaattc cacacatagt ttttgattcc gatttatatc gttgtcggtc
     2101 aacctgtatg cccgtttttt cgggcgcccc gagccttgta gagagtgggg ttatttttat
     2161 agttactgct tgtgggaggg agattatgtt atctcctcat cccatgtgac cgggttagcc
     2221 ggccagaatc actttttctt taccggacga cgccagcctt ctttctgagt ttgcggtaca
     2281 cggctgatag ctaatgcatt ttcgccgaca ttacgcgtca cagttgtacc cgcagcaatg
     2341 gttgcacctt tgcctactgt taccggggcc accagctgag tgtcggaacc gacaaacaca
     2401 tcgtcgccga taatggtctt aaatttattc gcaccgtcgt agttgcaggt aatggttccc
     2461 gcgccgatgt taacgttatc gccaatttcc gcatcgccca ggtaagtcag atgaccagct
     2521 ttggagcctt tacccagacg cgcttttttc atctcaacga agttaccgac gtgtgcacct
     2581 tccagcaact cagcaccagg acgcaaacgg gcaaacgggc caatggtaca ggccgctgcc
     2641 agattcgcat cttccacaac ggtatacggg ctaatttcgc aatcatcgcc aatcacgctg
     2701 tttttaatca cgcaaccggt gccgattttc acgcgatgac cgagagtcac gttgccctcg
     2761 atgataacgt tagtatcaat ttcaacatcg cgcccgtgag taagcgtacc acgcagatca
     2821 aaacgcgccg gatcgcgcag cataacgcct gctaacagca gtttttcagc ctgttcggac
     2881 tgataaacac gctccagacg ggagagttgt aggcggttat tcacgccttc tacttcgctt
     2941 aaacgttgcg gatgaacggc gacgatttca cgcccttcct gatacgccag tgcaataatg
     3001 tcggtgatgt agtattcgcc ctgagcatta ttgttggtca gcttcgccag ccagcgtttc
     3061 atatctgcgc cgttagcgat cagaatgccg gtgttgatct cctgaatctg acgctgctcg
     3121 tcggtggcat ctttgtgctc aacaatgccg gtaactttgc cgttttcacg ggtgatacgt
     3181 ccataaccgg tcggatcatc cagtttcacc gtcagcagac caatgccacc ctgcggttta
     3241 gcatcacgca gacgctggag tgtttcgaca gagatcagcg gcacgtcgcc gtagagcatt
     3301 aaaatgtctt catcatcggc aaagaaaggt gcggcctgct gcatggcatg acccgtaccc
     3361 agctgctctg cctgaagcac ccagttcagg ttgtcgtctt tcagcgcctg ttttagcaga
     3421 tcgccgccgt gaccgtacac caagtgaacg tgcgctgcgc ctaattcatt cgcagcatca
     3481 atgacatgct gaaccatcgc tttcccggca agggtatgca gcactttcgg aagatcggaa
     3541 tacatgcgcg tgcctttacc tgcggcaagg atcactacgc tcatagcatt attcaacata
     3601 cgcgtcctga ctgtaatttg agaacgaatt taaaccgctt caccttgaaa aaactacatt
     3661 tttttcatcg tgaaatggac agaggataaa ttgttcaata acggattatc cccgctgacg
     3721 aaagcgccat tttcgaccat cgtcacgcct tttgtctgca gaaaattaag gcagtacctt
     3781 catttaagcg tcaggtggat gtttttgctc ttatttcgat caatgaataa acgggaaaac
     3841 agggggtttt atatcagcag gatctatgtg aacgctattc aggacgggtc acacgcgcaa
     3901 aaaaaagcca gcctgtttcc agactggctt ttgtgctttt caagccggtg ttacatcgct
     3961 tttttggtca actcgataac gcgcagctgc gcgatcgctt tggccagttc cgcagacgcc
     4021 tgagcgtaat ctacgtcgcc gtgagagctg ctaatgtgct cttcagcctt acgtttcgct
     4081 tccatggctc gcgcttcgtc gagatcctgg ccgcgaattg cggtgtcggc cagaacggtc
     4141 acgttgccag gctgcacttc aagaatgccg ccagacagat agataaactc ttcgtgaccg
     4201 tgctgtttca cgatgcgaat cataccaggc ttaatggcgg tgagcagcgg tgcgtggcca
     4261 gggtagatcc ccagttcacc ttcgctaccc gttacctgga ttttctcgac cagaccagag
     4321 aacatttgtt gctctgcgct gacgacgtcc aggtggtaag tcattgccat atcaccctcc
     4381 gattaaggcg ttaaagtttt ttggcttttt ccacagcttc ttcgatggaa ccgaccatgt
     4441 agaacgcctg ctccggcagg tgatcgtatt cgccttccat gatgccttta aagccacgga
     4501 tggtgtcttt cagggagacg tatttacccg gagaaccggt gaatacttct gccacgaaga
     4561 acggctggga caggaagcgc tggatcttac gagcacgtgc taccaccagt ttgtcttctt
     4621 cagacagttc atccataccc aggatggcga tgatgtcttt cagttcctga taacgttgca
     4681 ggatggactg aacgccacgc gcggtgtcgt agtgttcctg accaaccacc agcgggtcca
     4741 gctgacggct ggtggagtcc agcgggtcaa cggccgggta gatacccaga gacgcgatct
     4801 gacggctcag taccacggtt gcgtcaaggt gcgcaaaggt ggttgccgga gacgggtcag
     4861 tcaagtcatc cgcaggtacg tataccgcct gtacggaggt gatagaaccg gttttggtgg
     4921 aggtgatacg ttcctgcaga acgcccatct cttccgccag ggtcggctga taacctaccg
     4981 ctgaaggcat acggcccagc agtgcggata cttccgtacc ggccagggtg taacgataga
     5041 tgttgtcaac gaacagcaga acgtcacgac cttcgtcacg gaatttctca gccatggtca
     5101 gaccggtcag agctacgcgc agacggtttc ccggcggctc gttcatctgg ccatacacca
     5161 gggatacttt gtcgataacg ttggagtcgg tcatttcgtg gtagaagtcg ttaccctcac
     5221 gagtacgttc acctacgccc gcaaacacag agtaaccgga gtgctcgatc gcgatgttac
     5281 gaatgagctc catcatgttt acggttttac ccacacccgc accaccgaac agaccaactt
     5341 taccgccctt agcgaacgga cacatcaggt cgataacttt gataccggtt tccagcagtt
     5401 cctgagagtt tgacagctct tcgtaggaag gtgctgcgcg gtgaatcgcc caacgctctt
     5461 cttcaccgat ctcgcctttc atgtcgaccg gttcacccag tacgttcatg atacggccca
     5521 gagtcgcttt acctaccggg acttcgatcg ggtgttcgag gtcttttaca tccagaccgc
     5581 gacgcagacc gtcggaggaa cccattgcga tggtacgcac gataccgccg ccgagctgct
     5641 gctgaacttc cagcaccaga cgctcattac cattttgcac ctcaagagca tcgtacacgc
     5701 gcggtacggc atcctgaggg aattcgacgt caactacggc gccgattacc tggacaatct
     5761 ttccagtagc catcttaaat cctctacgaa ataacctgtt taaaccgcgg cggcccccga
     5821 gacgatctcg gtgagttcct gagtaatgct ggcctgacga gctttgttgt ataccaactg
     5881 cagctcttta atcaggctgc cgccattgtc ggtcgcggct ttcatcgcca ccatacgggc
     5941 ggcctgctcg ctggccaggt tttcaaccac gccctgataa acctgagatt cgacataacg
     6001 acgcagcagg gtatccagca acgccttcgg atcgggttcg tacaggtaat cccaggattt
     6061 atgtttcaga tcatcatcat ctgatgccgg taacggcagc agctggctga tggtcggaac
     6121 ctgagacatg gtgttaataa atttgttgct gacaatgtaa agcttgtcca gacggccttc
     6181 gtcgtaggcc tgcaacatca cttttaccgg accgatcagt tcggacaggg aagggttatc
     6241 ccccatgccg gtgacctggg caacaacatt gccgcccacg gagttgaaga acgacacgcc
     6301 tttcgagccg atcattgcga ggtcgcattg aacgccttta tcggtccagg tcttcatttc
     6361 cgccagcagt tttttgaaca ggttaatgtt caaaccaccg cacaaaccac ggtcggtcga
     6421 caccaccagg tagcccacgc gtttaacgtc gcggtcttcc aggtaagggt gcttatattc
     6481 cagattaccg tgtgcaaggt gaccaatcac tttgcgcatg gtttctgcat aaggacggct
     6541 ggccgccatg cgatcctgcg atttacgcat tttggaagcg gcgaccatct ccatcgcttt
     6601 agtgatcttt tgcgtgttct ggacgcttgc gatcttacta cgtatctctt ttgcgccggc
     6661 catgagcttc tcctcaatgc cttgcggcct gccctaaggc aagccgccag acgttaccag
     6721 gattgggttg ctttgaagga atcgaggatg cctttcagct tgccttcgat ttcgtcgttg
     6781 tagccaccgg tctggttgat ctcttgcatc aacggagcgt gatcacggtc gacgtaagcc
     6841 agcagagcgg cttcgaagct gccgattttc gacagttcaa catccgccag gtaaccacgt
     6901 tctgctgcga acagaaccag agactgctgc gcaacggaca tcggcgcata ctgtttctgt
     6961 ttcagcagtt cggtcacttt ctgaccgtgg tcaagctgct tacgagttgc atcgtcaagg
     7021 tcggatgcaa actgagagaa cgctgccagt tcacgatact gtgccagagc ggtacggata
     7081 ccaccggaca gttttttcat gatcttggtc tgtgctgcac caccaacacg ggatacggaa
     7141 atacccgggt taaccgcagg acgaataccg gcgttgaaca ggttggtttc caggaagatc
     7201 tgaccatcgg taatggagat tacgttggtc ggaacgaacg cagaaacgtc acccgcttga
     7261 gtttcgataa tcggcagcgc ggtcagtgaa ccggttttcc ctttcacttc acctttggtg
     7321 aaagcttcaa cgtattcggc gttaacacgc gcagcacgct ccagcagacg agagtggagg
     7381 tagaatacgt cgcccgggaa tgcttcacgt cctggcggac gacggagcag cagggagatc
     7441 tgacggtaag caacagcctg tttagacagg tcatcgtaaa tgatcagcgc atcttcaccg
     7501 cggtcacgga agtattcgcc cattgcgcaa ccggcatacg gtgccaggta ttgcagtgca
     7561 gcggattcag acgcggttgc taccacaacg atggtgttag ccagtgcgcc gtgctcttcc
     7621 agtttacgta ccacgttaga aatggtggac gctttctggc cgatagcgac atagatacat
     7681 ttgataccgg aatcgcgctg gttgatgatg gcatcgatag ccagtgcggt tttacctgtc
     7741 tgacggtcac cgatgatcaa ttcacgctga ccacgaccga ttgggatcat ggagtcaacg
     7801 gctttataac cggtctgtac cggctgatct acggactgac gttcgataac gcccggagcg
     7861 attgcttcta cagcagagaa gccgtcgtga tccagcggac ctttaccgtc gattggtgca
     7921 cccagagtgt taaccacacg gcccagcagg ccacggccaa ccggaacttc caggatacgg
     7981 ccagtacact taactttcat gccttcggca aggtcagcgt acggacccat aacaaccgca
     8041 cctacagagt cgcgctcgag gttcagtgcg atagcgtaac ggttacccgg cagggagatc
     8101 atttcaccct gcatacaatc ggccaggccg tgaatgcgga taacaccgtc acttacagaa
     8161 acaatagtac cttcgttgtg agcttcactc acaacattga actgagcaat gcgctgcttg
     8221 atcagttcgc tgatttcggt ggaattcagt tgcatgctcc agtcccctta agactgcaag
     8281 acgtctgcaa ggcgctcaag acgaccgcgt acgctgccat caatgaccat atcacccgct
     8341 cggatgataa cgcctgccat tacagactta tcgattttgc aattcagctt aactttgcgt
     8401 gacagacgtt tttccatcgc agcagaaatt ttcgcgagct gttgttcact cagtgcagca
     8461 gcggaaatga cgtctacctc agcggtagcc tcactcacgg cacgcaggtg aataaactgc
     8521 tccagaacat ccgggagcgc tttaagacga ccattttcag ccataacccg aatcaggttc
     8581 tgaccgtttt cgtccagttg ctcaccacaa actgcgataa acgactcggc gagcgtttct
     8641 ggcgccagtg cgccagagag aagctctgcc atttgttcgt ttttggttac ctcggcggca
     8701 aacgccagca tgtcctgcca gcgttctaca ctttggtgtt cgacggcaaa gtcaaaagct
     8761 gctttggcgt aggggcgagc taccgtaata aattcagaca tcagcccctc cctccttaca
     8821 gttcagcgac aagtttatcc acgatgtcgc tgttagcagc ttcatccacg gaacgttcga
     8881 tgatcttctc ggcgccagca acagccagga tagcaacttg cttacgcagc tcttcacggg
     8941 cacgtttacg ctcggcttca atttccgcct gcgcctgggc cacgatttta gtacgttcct
     9001 gttctgcctc agctttcgct tcgtccagaa tctgcgagcg gcgtttgttt gcctgctcga
     9061 tgattacctg ggcttccgct ttcgcttttt tcagctggtc ggtcgcgctg gcctttgcaa
     9121 ggtcaaggtc cttatgtgct cgttctgcgg aagcaaggcc gtcagcaatt tctttttgac
     9181 gtttttcgat ggctgccatt aatggcggcc atacgtactt catgcagaac agaacgaaca
     9241 ggacaaacgc gatggcctgg ccgaggattg ttgcgttaag attcacagca caatgcctct
     9301 atttagttaa cgttctgata ttgctcttta aataaaagca acgcttacta cgcgacagcg
     9361 aacatcacgt acagacccag acctacagcg atcatcggga tagcatccac cagacccata
     9421 acgataaaga actgagtacg cagcagagga atcagatcag gttgacgcgc tgcgccttcc
     9481 aggaatttac ccccgaggat gccgataccg atcgcagcac cgattgccgc cagacccatc
     9541 atcacagcgg cagccatgta cagcagatcc atattcaggt tttccatgac agtctccagt
     9601 ttgtttcagt taaaacgtag tagtgttggt aaattaatgt tcttcagacg ccatcgacag
     9661 atagacgatc gtcagaacca tgaagatgaa ggcttgcagc gtaatgatca ggatgtggaa
     9721 aatggcccac ggcacattca ggatccactg tgaccaccac ggcaacagac cagcaatcag
     9781 aatgaaaatc agctcaccgg catacatgtt accgaacagt cgcaaaccga gtgaaactgg
     9841 tttggacagc aggcttaccc cttcaaggat taagttgaca ggaatgaacg cccagtgatt
     9901 gaacggctgc agcgtcaact ctttcgtgaa gccgccgatg cctttcattt tgatgctgta
     9961 gaacagaatc aggataaata cgcccagtgc catagacagc gttacgttca cgtccgcaga
    10021 cggaaccaca cgcagtgcag gcagacccag tacatgttca gcaatgtacg gcagcaggtc
    10081 gataggcagt aaatccatca ggttcatcag gaatacccag acgaagatcg tcagggccag
    10141 cggagcaatc agcttgcttt tgccatggta catgtctttc acgctaccat taacaaagcc
    10201 gatcaccagc tcaatcgcgg tctgaaactt acctggcaca ccgctggtcg cctttttggc
    10261 tacgctacgg aataaaacca ggaacaacag acccagcacc accgagaaga acatggagtc
    10321 aatattgatt gtccagaagg tggctggggg gttttgtgga tccaccagcg agaatgtacg
    10381 caggtccagc tgaaggttat tcaggtggtg tcctatgtaa tcctgcggcg tcatattttc
    10441 tgaagccatg atgcctttta ccctttgttg ttaattacag ccggtgccag tatctgaacc
    10501 accagcacca aaacccacgt aacgatcagc ggcaagaata ccgcctttaa aaccgccaac
    10561 gccaccacca gtaacaccaa catcgccaga actttgaaag cttcgccaaa tgcgaatgtc
    10621 caggccaccc ggcctttcgc tggtgtatgc gcctggtgac gccaggcaaa tatcataaac
    10681 aaaacgttag gcagaaagac tgccaggccc ccgcttattg cagagacgcc ccagaagggg
    10741 tctttgaggc tgaacagcaa tccacttgct atcaccacca gtaactgaac gagcagaagc
    10801 ttccgagcaa cgtttcgact cacgagcgac acagacatca cgtttttcac tcctgctccc
    10861 ttcgaggtat gccgcgtgtc gtataaaact ttctttaagg cttagagtca agcatcaaaa
    10921 agcggtcaaa ttatacggtg cgcccccgtg atttcaaaca ataagtagcc aaaaggtgaa
    10981 taaatgttta aatttttttc cagtgcatac aattgcgact tttctactaa ccctgttcga
    11041 tcatgaaaaa ctgtaaataa cgcgtaaaca ctggtgataa agcgtgcttc agatcacata
    11101 ttgcgcatgt tcgcgcacag catatttatt tacttggcaa atgatgcctt tgcaagttta
    11161 tgatatttca gtctaaaaac agatactgtt ttaataaatg acatttacac aacaaaaacc
    11221 acccattgac atttttaata atgttttaac agccaatgat gattcttagc gccgattttt
    11281 agcagactga tattttcact aatgacttat tttctgctta ccaaaaaaag ccacgttatc
    11341 ttgttgatgc aaaagagtga acgtggcgtt aaatgtaacc agttatatca gtagaaaacc
    11401 tggttgttgt taacagtcta accggtcaat tttttatgat ttttttgata aaaattaaat
    11461 tttatttgct ttaatcacca ccagatggcg ttcgccatcc agggctggaa cctgaagttt
    11521 aaccactgat tcaacctgat attcttcggg caacaaagcg atttcatctt ccggcatttg
    11581 ccctttcagc gcgtagaaac ggccttgctc accaggaaga tggtggcacc agctcaccat
    11641 atcgttcaga gaggcaaaag cgcggctaat tacgccatca aatggcggct ctgaaggaaa
    11701 ctcttctacc ctgctctgta ctggctcaat attctccagt ttaagctcat gttgcacctg
    11761 acgaaggaaa cgcacgcgtt taccaaggct atccaacaga gtgaaatggg cttcaggacg
    11821 cacgatagag agtggaatgc caggcagtcc aggaccggtg ccgacatcga taaatcgttc
    11881 accttgcaga tacggtgcca ccacaatgct atcgagaata tggcgtacca gcatctcatt
    11941 aggatcgcgg accgaagtca ggttgtacgc tttgttccat ttatgcagca tattcacgta
    12001 ggcaataagc tggtttttct ggtgatcggt aagcgaaata cctgcgtctt tcagcagtaa
    12061 ggagagtttg ttgagcacgg tgattacctg ttcttgatgc gttgcctggt aagcgggtgc
    12121 ttaccaggca tttttaatgc gttatgcgct acgacgcagc ataccctgtt ttttcagcca
    12181 caccagcaga atggagatgg ccgcaggcgt gacgccagaa atacgcgaag cctggccgat
    12241 agaggccggt ttgtgatcgt taagtttggc gatcacttcg ttagaaagac cggatacctg
    12301 gcggtaatcc agtgtcgcgg gtagcaaggt gttctcgtta cgcagctgct tttcgatctc
    12361 atcttgctgg cgcgcgatat aaccttcgta tttaacctga atctcaacct gttccgccgc
    12421 ctgttcgtct gtcaacgcag gggcaaacgg cgtcagcgtg gttaattttt cataagtcat
    12481 ttccggacga cgcagcagat cttcaccact ggcttcacgg gaaagcggcg cagtcaggtg
    12541 agcattcact tcggctgcag cttccgccga cggggttacc caggtcgatt tcagacgctg
    12601 acgctcacgc tcgatattct caagtttctc gttaaagcgc gcccaacgtt cgtcatccac
    12661 caggcccagt tcacgaccga tttcagtcaa acgcagatcc gcattatctt cgcgcagcat
    12721 cagacgatat tctgcgcgcg aagtaaacat acgatacggt tctttggttc ctaaagtgca
    12781 caggtcatca accagtacgc cgagataggc ctgagaacgc gccggagccc aaccttcttt
    12841 gtcagcagac agacgggcag cgttaagacc ggccagcaaa ccttgcgcag cggcttcttc
    12901 gtaaccggta gtgccgttaa tctgaccagc aaagaacagc ccctggataa acttgctctc
    12961 cagcgtcggt ttcaggtcgc gtggatcgaa gaagtcatac tcaatggcat aacccggacg
    13021 cacgatcttc gcgttttcca tcccctgcat ggagcggacg atttgcatct gcacatcgaa
    13081 cggcaggctg gtggagatac cgttcggata aatttcgtta gaggtcagcc cttccggttc
    13141 aaggaagatc tgatgctgat ttctgtcggc gaagcgcatg actttgtctt cgatcgacgg
    13201 gcagtagcgt gggccgacac cttcgatcac ccctgcgtac attgggctac gatcgaggtt
    13261 actgcggatc acatcatggg ttttctcgtt ggtatgagtg atataacacg gcacctgctg
    13321 gggatgctgg gacgcattgc ccataaacga gaataccggc attgggttat cgccatgctg
    13381 ttgcgcaagc acgctaaagt cgatggttcg agcatcaata cgcggtggtg tcccggtttt
    13441 cagacgacca acgcgcagcg gaagttcacg caaacggcga gaaagcggaa tggacggcgg
    13501 atcaccagca cggccaccgc tgtagttatc cagaccgata tgaattttac cgtcgaggaa
    13561 cgtcccaacg gtgagcacaa cggctttggc acggaacttc agtcccattt gggtaacagc
    13621 accgaccacg cgatcgtttt cgacaataag atcttcaacc gcctgctgga agatcatcag
    13681 attcggttgg ttctccagcg ccgtacgtac cgcctgacgg tagagcacac gatccgcctg
    13741 agctcgggta gcgcgaacag ccggtccttt acttgcgttt agtatcctaa actggatacc
    13801 cgcctgatcg atcgctttcg ccatcagacc gccgagtgca tccacttctt ttaccagatg
    13861 tcccttccca ataccgccga tcgccgggtt gcagctcatc tgccccagag tgtcgatatt
    13921 gtgtgtcaaa agcagagtct gttgacccat acgcgccgca gccatcgcgg cctcggtgcc
    13981 tgcatgaccc ccgccaatga tgatgacgtc aaaaggatcc ggataaaaca tggtgattgc
    14041 ctcgcataac gcggtatgaa aatggattga agcccgggcc gtggattcta ctcaactttg
    14101 tcggcttgag aaagacctgg gatcctgggt attaaaaaga agatctattt atttagagat
    14161 ctgttctatt gtgatctctt attaggatcg cactgccctg tggataacaa ggatccgact
    14221 tttaagatca acaacctgga aaggatcatt aactgtgaat gatcggtgat cctggaccgt
    14281 ataagctggg atcagaatga ggggttatac acaactcaaa aactgaacaa cggttgttct
    14341 ttggataact accggttgat ccaagcttcc tgacagagtt atccacagta gatcgcacga
    14401 tctgtatact tatttgagta aattaatcca cgatcccagc cattcttctg ccggatcttc
    14461 cggaatgtcg tgatcaagaa tgttgatctt cagtgtttcg cccgtctgtt ttgcaccgga
    14521 atttttgagt tcagcctcga gtttatcgat agccccacaa aaggtgtcat attcacgact
    14581 accaataccg attgcgccaa agcggaccgc agaaagatcg ggcttctgtt cctgcaatgc
    14641 ttcatagaaa ggagaaaggt tgtccggaat atctccggca ccgtgggtgg agctgataac
    14701 cagccagatc cccgaggcag ataaatcttc caacagcgga ccgtgcagcg tttcggtggt
    14761 aaaacccgcc tcttccagct tttcagccag gtgttctgct acatattcgg caccgccgag
    14821 ggtgctgccg ctgataagag tgatatctgc cataaaccgc cacctttatt aagagtggcg
    14881 tattgtacgc tgtgaacgcg ttgggatcta cctgtggaaa agtatgggat taaaaaagcc
    14941 gatcagggct tgatggtacg catgatcggg ttctgcagga cgatcagtgt ctcggtggac
    15001 tgaatttcat caattgtttg gatcttgttg ataagtacat gctggagagc atcgatcgaa
    15061 cggcacatca cttttataaa gatgctgtag tggccggttg tgtaataggc ttcagtgact
    15121 tcatcgaggc tttccagttt cgccagcgcg gaaggatagt ctttggcgct ctttaatata
    15181 atgccgataa agcagcctac gtcataaccg agctgcttcg ggctgacatc aatacgcgcc
    15241 ccggtaatga tccccgcctg cttcattttc tctactcgaa cgtgaatcgt ccccggactg
    15301 acgccaaatt gtttcgccag ttctgcgtaa gcggtgcgcg cattgcccat taatgcttcc
    15361 aggatgccac ggtccagatt gtcgatcaga taattttcca taggattttc ttatgcagat
    15421 tgatgattca ttctatttta gccttctttt ttaatgaatc aaaagtgtgt taggcttttt
    15481 attgaatgat tattgcatgt gtgtcggttt ttgttgctta atcataagca acaggacgca
    15541 ggagtataaa aaatgaaaac cgcttacatt gccaaacaac gccaaattag cttcgtgaaa
    15601 tctcactttt ctcgccaact ggaagaacgt ctggggctga tcgaagtcca ggcgccgatt
    15661 cttagccgtg tgggggatgg cacgcaggat aacttgtcgg gctgtgaaaa agcggtgcag
    15721 gtaaaagtga aagctctgcc tgatgcccag ttcgaagtgg ttcattcact ggcgaagtgg
    15781 aaacgtcaga ccttagggca acacgacttc agcgcgggcg aagggctgta cacgcacatg
    15841 aaagcccttc gccccgatga agaccgtctt tctccgttgc actcggtcta tgttgaccag
    15901 tgggactggg aacgcgtaat gggcgacggt gagcgtcaat tctcgactct gaaaagcacg
    15961 gtagaggcga tctgggcggg aattaaagca accgaagctg cggttagcga agagtttggc
    16021 ctggcaccgt tcctgccgga tcagatccac ttcgtacaca gccaggagtt actgtctcgt
    16081 tatccggatc ttgatgccaa agggcgtgag cgggcgatag cgaaagatct tggtgcggta
    16141 ttccttgtcg ggattggcgg caagctgagc gatggtcatc gccacgacgt gcgcgcaccg
    16201 gattatgatg actggagcac cccgtcagag ctgggccatg cgggtctgaa cggcgatatt
    16261 ctggtgtgga acccggtact ggaagatgcg tttgagcttt cttccatggg gatccgcgtg
    16321 gatgccgaca cgttgaagca tcagttggcg ctgaccggtg atgaagatcg tctgcagctg
    16381 gaatggcatc aggcgctgct gcgcggtgaa atgccgcaga ccatcggcgg cggtatcggc
    16441 cagtctcgtt tgaccatgct gctgctgcaa ctgccgcata tcggccaggt tcagtgtgga
    16501 gtatggccag ctgctgttcg cgagagcgtc ccttctctgc tgtaataatt tatcgccgcc
    16561 agcgtctgag caggcggctt cgcatcccgg tatcaaagcg ccagatatga tcgaaaatgc
    16621 gcatgatgcc gggtttgccg tgtgccgaca tcgccacggc atgaaagcga tgctgatgta
    16681 cccgctgcag ctctttcact ttactcgtca cgtcgtcagg caaccgctgg gcgataaaat
    16741 cagaaatcac caccgcatcg gcatcaaacc attccctgct ttgcaagcgt tccataatgg
    16801 cgcgaaaaca actggcaaga tcggtgccgc cacgaaactg ctggcttaaa aaacggattg
    16861 cttgttcgat gccttgtggg cctgaaagct cataacggac gatctcggtg gaaaatagca
    16921 taatatagca gcgccggttt tcagcgagag caatgcgcat caaagccagg cagaacgctt
    16981 tcgcacactg ttcattaaag ccgcccattg agccggaagt atccacgcag acaataaacg
    17041 gtccgcgcgg ctgttcgtcg taatctttat gcaccaccgg gcgttcgatc actttttcac
    17101 gccacgactc accgtgcagg cgataggtga gcaactgttt ttccaccagc cgacggtaaa
    17161 actcatactc cagttccgtt atccctagtg tcgccagttc tggcggcagg agacgtaaaa
    17221 tatcatcgct ttgttgcaga ccatcaacct gctcaggaac agttgccggt tcgcgcacca
    17281 tggtgcggaa ggtttccatc tgcgcatcgt tgcgcggtat tgatttggct tcccgggaac
    17341 gccccagttg ttctgccagg cgtttcagtt ccggctgttc gttaagaaat tcaccgtatt
    17401 tcacaatcaa ctgatagtcg ccacgtttaa gctgaccggc gctcatatcc cacagacgac
    17461 cagctgcggt attgttatct gcgagaatcg gttcaagttg tccgctcagc gtcatgcgtt
    17521 cctgaacttc acttaacagt tgttcgcgtt cttcttctaa tagctgttga tttaacgtcg
    17581 ttgcttgcac gatcagactt aaacgccaac gctggagaaa aagtgtgtgt aacgctgaag
    17641 tgatcgcgct gttagcatca accaactgtc gggcttgttc tgcccatggg gaattcagac
    17701 gatgcagtaa gtccaggatc tgtggtagct gcacaataaa ctgcggcgtg gagaggagct
    17761 ggctttgctg atagcacatc acctcttcgg tgagttcctg tgggactcgg gcatctttca
    17821 gccgattacg cagcgcctca cgccagcggg gaacatcatc agtgattgcc gccttcagtc
    17881 gtgggaattt ttcaaagaag accgccagct gcggtgaggc cagcagcgcg atgatcatct
    17941 cttcgatcaa tccctcttcg ctgacggcca gcatcacatt aagcgtatcc agcgttagca
    18001 ttgttgtgcc tggcgaatct gtgcgccgac atcctgtagg ctggcttcaa tgcgacctaa
    18061 ccagtcgcca gggataaaca gacatttttg ctgttcgctg aataaagcgt gttgcttgcg
    18121 ccagtcgctt tctagctctt ccagttgttg ttttatttca cctggcagac cttcagccga
    18181 tgaaccggga agtgccagcg tactgccttg taaacttaca tcgcgaacga caagatgttg
    18241 ggcgctatca acttccagat tcagtttctg ggcaaagccg ataccgttca gtttaccgcg
    18301 aatttcacca cctttgctca gccactgttc cagcgcgcta cgctcaaagg agatatgaac
    18361 cacttccata tcatgcagtt ttaacggttt ttgcagcagc agagtcagag tggaagcagt
    18421 aacgttaaca ggaagttgat actgctgacg acggctgaaa atgccgccca gacgaattac
    18481 cgttaatgct gttttatcgc tttgttgctg ctggagttgc aggtgacgtt gcacaatcgc
    18541 gcccaggcgg gtcaacatcc cttgctgttg ccaggcatga ccggtcatca atacatcaat
    18601 ttgttgttgt atcaaattca ggctttgcgc gtcataccac aggcaatctt tcagcaaaat
    18661 gagatctacc ggggcaacag cactgcgacc gctaaaaaag gcgctggcct gcaataagcg
    18721 gatcgctttt ttccagcgac gatccgagac ataaggcgca tccggtaatt tatccagttg
    18781 ctggcgcagc ataaaaatca gctcaaatac atgatcgggc agcgtaattt caccaatctc
    18841 tttctgccag cgttcatatt cttcatctgt gacctgcaag gcatcaggaa ccggattgtc
    18901 gttttcatcc tgttgactgg tcagcatgga gcggaaattc gctttatcct gcactttatc
    18961 taaccacaga cgaatcagca tgcggtcata taacgcttcc agactgctgt ctgcttccgg
    19021 cagctcgttg gaggccgcca ccagcagacg catcgggatt ttttctacgt gtgcaccgtt
    19081 gcggaactgg cgctcgttaa tggcggtgag caaggtatta agaattgccg ggcccgcttt
    19141 ccagatctca tccagaaaga caatttctgc ttccggcagg taaccgctgg ttaaacgttc
    19201 atagcgccct tcatctttta gcgcctgaat agaaagggga ccaaaaactt cttccggcgt
    19261 ggagaagcgg gtcatcagat attcaaacgc gcgggcattc tgaaaggcga attttaagcg
    19321 ccgggcgatc aaacttttgg caatacctgg cgggccaagg aggaacacac tttcaccact
    19381 taatgccgct aataaacaca agcggatggc gtggctacgt tcataaagcc ccttttccaa
    19441 cgaactgctc aggcgggaaa ttctttccgc taataaatga gggtgagcca taatgaagtg
    19501 gcgtcctttc gtcaaaagtt ctgcgtaaat tgcgagtata gacgtttctt gctggtggct
    19561 aaaatagtct caaagggggg gtatttttct ttgagccagg ttaatgtggc cgcatttagg
    19621 agtacgattt tgccgttaat cgtgcatact gtgcgctttt ttgtgggcca agggactaag
    19681 cacacatttc atatttcaac gaaagactag tctatgagca ctgataataa gcaatcattg
    19741 cccgcgatta ccctcgcggc gattggagtt gtctacggcg atattggtac cagcccgtta
    19801 tatacacttc gtgaatgttt gtccggccag tttggttttg gcgttgaacg cgatgccgtg
    19861 tttggctttt tatcgctgat cttctggctg ctaatctttg tggtttccat taaatatctc
    19921 accttcgtga tgcgggcaga taacgccggt gaagggggga tcctgacgtt gatgtcgctt
    19981 gccgggcgta atacgtcggc gcgaactaca tcaatgctgg tgattatggg gctaatcggc
    20041 ggcagctttt tctatggtga agtcgtcata acacccgcta tttcggtgat gtcagccatt
    20101 gaaggtctgg aaatcgtcgc cccgcagctg gatacctgga tagttcccct ctcaattatc
    20161 gttctcactt tattatttat gattcaaaag catggcacgg ctatggtcgg taagctgttt
    20221 gcgccgatca tgctgacctg gtttttgatt ctggcaggac tggggttacg tagcattatt
    20281 gctaacccgg aagtgctgca tgcgctgaat ccgatgtggg cggtgcattt cttccttgaa
    20341 tacaaaacgg tttcttttat tgcattaggg gcagtggtgc tgtcgattac gggggtcgag
    20401 gcgctgtatg ctgatatggg gcactttggt aagttcccta ttcgcctggc gtggttcacc
    20461 gtcgtattac cttccttgac ccttaattac ttcggtcagg gagcgttgtt gttaaagaac
    20521 ccggaagcga ttaagaaccc gttcttcctg ttggcaccgg actgggcgct gatcccactg
    20581 ctgatcatcg ccgcactggc gacggtaatc gcctcgcagg cggttatctc tggcgtcttt
    20641 tcattgacgc gtcaggcggt acgtctggga tatttgtcgc cgatgcgcat tattcacacc
    20701 tccgaaatgg agtcagggca aatctatatt ccgtttgtga actggatgct ctatgtcgcg
    20761 gtcgtgattg tgattgtcag ctttgagcac tccagcaacc tggcggcggc gtacgggatt
    20821 gcggtgaccg gaaccatggt gctgacgtct attctctcga ctaccgtggc acgtcagaac
    20881 tggcactgga ataagtattt tgttgcgctg atcctgattg ctttcctttg tgtcgatatt
    20941 ccattgttca ccgctaacct cgataaactg ctctccggcg gctggttgcc attgagcctc
    21001 ggtactgtga tgtttatcgt gatgaccacc tggaagagcg agcgtttccg cttgctgcgg
    21061 cggatgcatg aacatggtaa ctctctggaa gcgatgattg cttcgctgga gaaatcaccg
    21121 cctgttcgcg tgcccgggac cgcggtgtat atgtcgcgtg caatcaacgt cattcccttt
    21181 gcgctgatgc ataaccttaa acataacaag gtattgcatg agcgggtgat tctgttaact
    21241 ctgcgcaccg aagacgcgcc atatgtccat aacgtccgtc gggtacagat tgaacaactg
    21301 tcgcccactt tctggcgcgt ggtggcaagt tatggttggc gagaaacgcc aaacgtggaa
    21361 gaagttttcc accgctgcgg tctggaagga ttaagttgcc ggatgatgga aacctccttc
    21421 tttatgtcgc atgagtcgtt gatcctcggc aaacgcccgt ggtatttgcg tctgcgcggc
    21481 aagctgtact tgctgctgca acgtaatgcg ctgcgtgcac cagatcaatt tgaaatcccg
    21541 ccaaacaggg ttatcgaact gggtactcag gtcgaaatct aacgccagac gcctcctttc
    21601 ttcataaggg ggcgtttttg ttttcatggt taatcaccat gtaaaacgtt tcgaggttga
    21661 tcacatttcc gtaacgtcac gatggttttc ccaactcagt caggattaaa ctgcgggtca
    21721 gcgaaacgtt tcgctgatgg agaaaaaaat gaaaaaaggc accgttctta attctgatat
    21781 ttcatcggtg atctcccgtc tgggacatac cgatacgctg gtggtgtgtg atgctgggtt
    21841 acccatcccc aaaagtacaa cgcgtatcga tatggcatta acccagggtg taccttcttt
    21901 tatgcaggtg ctgggcgtcg tcacaaatga aatgcaggtc gaggctgcca ttatcgcgga
    21961 agagatcaaa caacataatc cgcaactcca cgaaacgttg ctcactcacc ttgagcagct
    22021 gcaaaaacac cagggaaata ccattgaaat tcgttacacc acgcatgaac aattcaaaca
    22081 acaaaccgca gaaagtcagg cggtaattcg cagcggagaa tgttctccgt atgcgaatat
    22141 cattctctgt gctggcgtga cgttctgagg ccgtcatgga agcattactt cagcttaaag
    22201 gcatcgataa agccttcccg ggcgtaaaag ccctctcggg cgctgcgtta aatgtctatc
    22261 cgggccgcgt gatggcgctg gtgggcgaaa acggcgcggg taaatccacc atgatgaaag
    22321 tgcttactgg tatctatact cgcgatgccg gtacgctttt atggctgggg aaagaaacga
    22381 catttaccgg gccgaaatct tcccaggaag ccgggattgg gattatccat caagaactga
    22441 acctgatccc gcagttaacc attgccgaaa acattttcct cggtcgtgag tttgttaatc
    22501 gctttggcaa aattgactgg aaaaccatgt atgccgaagc ggataaattg ctggctaaac
    22561 ttaacctgcg ctttaaaagc gacaagctgg tgggcgatct ttccatcggt gaccagcaaa
    22621 tggttgaaat agccaaagtg ctgagctttg agtcgaaagt catcattatg gatgaaccga
    22681 ccgatgcgct gaccgatacc gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat
    22741 cgcaaggccg cggtattgtc tatatctccc accgcatgaa agaaatcttc gagatttgcg
    22801 atgacgttac cgtttttcgt gacggacaat ttattgctga gcgcgaagtg gcatcactga
    22861 ccgaagattc gctgattgag atgatggtgg gtcgcaagct ggaagatcaa tatccgcacc
    22921 tggacaaagc gccgggagat atccgcctga aagtcgataa tctctgcgga cctggcgtta
    22981 acgatgtctc ttttacttta cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg
    23041 caggtcgtac cgaactgatg aaagtgctct acggcgcact gccgcgcacc agcggttacg
    23101 tcaccctgga tgggcatgaa gtcgttaccc gttcaccgca ggatggcctg gcaaacggca
    23161 ttgtgtatat ctccgaagac cgtaaacgtg acggtttagt gttgggcatg tcagtaaaag
    23221 agaacatgtc gctgacagcg ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg
    23281 ccgatgaaca gcaggcagtg agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga
    23341 tggaacaggc aattggtctg ctttccggtg gcaatcagca aaaagtggcg attgcccgcg
    23401 gtctgatgac acgccccaag gtgttgatcc tcgatgagcc tacccgtggc gtagatgtcg
    23461 gcgcgaaaaa agagatctat caactgatta accagttcaa agccgatggc ttgagcatca
    23521 ttctggtgtc atcggagatg ccagaagtat taggcatgag cgatcgcatc atcgtcatgc
    23581 atgaagggca tctcagcggg gaatttactc gtgagcaggc cacccaggaa gtgttaatgg
    23641 ctgccgctgt gggcaagctt aatcgcgtga atcaggagta aaaaaatgac aacccagact
    23701 gtctctggtc gccgttattt cacgaaagcg tggctgatgg agcagaaatc gcttatcgct
    23761 ctgctggtgc tgatcgcgat tgtctcgacg ttaagtccga actttttcac catcaataac
    23821 ttattcaata ttctccagca aacttcggtg aacgccatta tggcggtcgg gatgacgctg
    23881 gtgatcctga cgtcgggcat agacttgtcg gtaggttctc tgttggcgct aaccggcgca
    23941 gttgctgcat ctatcgtcgg cattgaagtc aatgcgctgg tggctgtcgc tgctgctctc
    24001 gcgttaggtg ccgcaattgg tgcggtaacc ggggtgattg tagcgaaagg tcgcgtccag
    24061 gcgtttatcg ctacgctggt tatgatgctt ttactgcgcg gcgtgaccat ggtttatacc
    24121 aacggtagcc cagtgaatac cggctttact gagaacgccg atctgtttgg ctggtttggt
    24181 attggtcgtc cgctgggtgt accgacgcca gtctggatca tggggattgt cttcctcgcg
    24241 gcctggtaca tgctgcatca cacgcgtctg gggcgttaca tctacgcgct gggcggcaac
    24301 gaagcggcaa cgcgtctttc tggtatcaac gtcaataaaa tcaaaatcat cgtctattct
    24361 ctttgtggtc tgctggcatc gctggccggg attattgaag tggcgcgtct ctcctccgca
    24421 caacccacgg cggggactgg ctatgagctg gatgctattg ctgcggtggt tctgggcggt
    24481 acgagtctgg cgggcggaaa aggtcgcatt gttgggacgt tgatcggcgc attaattctt
    24541 ggcttcctta ataatggatt gaatttgtta ggtgtttcct cctattacca gatgatcgtc
    24601 aaagcggtgg tgattttgct ggcggtgctg gtagacaaca aaaagcagta ataacgacta
    24661 caggacatct tgaatatgaa catgaaaaaa ctggctaccc tggtttccgc tgttgcgcta
    24721 agcgccaccg tcagtgcgaa tgcgatggca aaagacacca tcgcgctggt ggtctccacg
    24781 cttaacaacc cgttctttgt atcgctgaaa gatggcgcgc agaaagaggc ggataaactt
    24841 ggctataacc tggtggtgct ggactcccag aacaacccgg cgaaagagct ggcgaacgtg
    24901 caggacttaa ccgttcgcgg caccaaaatt ctgctgatta acccgaccga ctccgacgca
    24961 gtaggtaatg ctgtgaagat ggctaaccag gcgaacatcc cggttatcac tcttgaccgc
    25021 caggcaacga aaggtgaagt ggtgagccac attgcatctg ataacgtact gggcggcaaa
    25081 atcgccggtg attacatcgc gaagaaagcg ggtgaaggtg ccaaagttat cgagctgcaa
    25141 ggcattgctg gtacatccgc agcccgtgaa cgtggcgaag gcttccagca ggccgtagct
    25201 gctcacaagt ttaatgttct tgccagccag ccagcagatt ttgatcgcac caaaggtttg
    25261 aacgtaatgc agaacctgtt gaccgctcat ccggatgttc aggctgtatt cgcgcagaat
    25321 gatgaaatgg cgctgggcgc gctgcgcgca ctgcaaactg ccggtaaatc ggatgtgatg
    25381 gtcgtcggat ttgacggtac accggatggc gaaaaagcgg tgaatgatgg caaactagca
    25441 gcgactatcg ctcagctacc cgatcagatt ggcgcgaaag gcgtcgaaac cgcagataaa
    25501 gtgctgaaag gcgagaaagt tcaggctaag tatccggttg atctgaaact ggttgttaag
    25561 cagtagtttt aatcaggttg tatgacctga tggtgacata aatacgtcat cgacagatga
    25621 acgtgtaata taaagaaaag cagggcacgc gccaccctaa cacggtggcg cattttatgg
    25681 acatcccgaa tatgcaaaac gcaggcagcc tcgttgttct tggcagcatt aatgctgacc
    25741 acattcttaa tcttcaatct tttcctactc caggcgaaac cgtaaccggt aaccactatc
    25801 aggttgcatt tggcggcaaa ggcgcgaatc aggctgtggc tgctgggcgt agcggtgcga
    25861 atatctcctt tattgcctgt actggcgatg acagcattgg cgagagcgtt cgccagcagc
    25921 tcgccactga taacatcgat attactccgg tcagcgtgat caaaggcgaa tcaacaggtg
    25981 tggcgctgat ttttgttaat ggcgaaggtg agaatgtcat tggtattcat gccggcgcta
    26041 atgctgccct ttccccggcg ctggtggaag cgcaacgtga gcgtattgcc aacgcgtcag
    26101 cattattaat gcagctggag tcaccactca aaagtgtgat ggcagcggcg aaaatcgccc
    26161 atcaaaataa gactatcgtt gcgcttaacc cggctccggc tcgcgaactt cctgacgaac
    26221 tgctggcgct ggtggacatt attacgccaa acgaaacgga agcagaaaag ctcaccggta
    26281 ttcgtgttga aaatgatgaa gatgcagcga aggcggcgca ggtactgcat gaaaaaggta
    26341 tccgtactgt actgattact ttaggaagtc gtggtgtatg ggctagcgtg aatggtgaag
    26401 gtcagcgcgt tcctggattc cgggtgcagg ctgtcgatac cattgctgcc ggagatacct
    26461 ttaacggcgc gttaatcacg gcgttgctgg aagaaaaacc attgccagag gcgattcggt
    26521 ttgcccatgc tgccgctgcg attgccgtaa cacgtaaagg cgcacaacct tccgtaccgt
    26581 ggcgtgaaga gatcgacgca tttttagaca ggcagaggtg acgcttggct acaatgaaag
    26641 atgttgcccg cctggcgggc gtttctacct caacagtttc tcacgttatc aataaagatc
    26701 gcttcgtcag tgaagcgatt accgccaaag ttgaagcggc gattaaagaa ctcaattacg
    26761 cgccatcagc tctggcgcgt agcctcaaac tcaatcaaac acataccatt ggcatgttga
    26821 tcactgccag taccaatcct ttctattcag aactggtgcg tggcgttgaa cgcagctgct
    26881 tcgaacgcgg ttatagtctc gtcctttgca ataccgaagg cgatgaacag cggatgaatc
    26941 gcaatctgga aacgctgatg caaaaacgcg ttgatggctt actgttactg tgcaccgaaa
    27001 cgcatcagcc ttcgcgtgaa atcatgcaac gttatccgac agtgcctacc gtgatgatgg
    27061 actgggctcc gttcgatggc gacagcgatc ttattcagga taactcgttg ctgggcggag
    27121 acttagcaac gcaatatctg atcgataaag gtcatacccg tatcgcctgt attaccggcc
    27181 cgctggataa aactccggcg cgcctgcggt tggaaggtta tcgggcggcg atgaaacgtg
    27241 cgggtctcaa cattcctgat ggctatgaag tcactggtga ttttgaattt aacggcgggt
    27301 ttgacgctat gcgccaactg ctatcacatc cgctgcgtcc tcaggccgtc tttaccggaa
    27361 atgacgctat ggctgttggc gtttaccagg cgttatatca ggcagagtta caggttccgc
    27421 aggatatcgc ggtgattggc tatgacgata tcgaactggc aagctttatg acgccaccat
    27481 taaccactat ccatcaacca aaagatgaac tgggggagct ggcgattgat gtactcatcc
    27541 atcggataat ccagccgacc cttcagcaac aacgattaca acttactccg attctgatgg
    27601 aacgtggttc ggcttagatt tacgctgtct tttgatcaaa ttattaccat cggttgtttt
    27661 cagaagcatg aacattgctg ctgaagcaac agtaataatg cccattgtga taaacgtata
    27721 gtggaattgt tcgacagtcg ttgtgccttc cattccttca taaacgcgaa ggacggccgc
    27781 acttacagct acgcctaaac tgatagacag ttgctgcgtg accgccagaa cactgttacc
    27841 gctgctggcg ttgtcatcgg tcagatcggc aagtgtgatg gtattcatcg cggtaaactg
    27901 cgtcgacata gccatcccta atataaacaa cggcaagatc agcatccata tcgccattgc
    27961 cggtgattgc aaagagaact gagcgatcat tagcccaata atcaccgtga tccccactaa
    28021 tgtatggcga tagcccagac gacgtaagac ttgggtaacc atcgattttg caataatgga
    28081 acctaacgct gtcggtgcca tcatacagcc ggcaataaac gcctgataac caaatcctac
    28141 ctgtaacatc aatggcataa ggaacggtac acagccggtc cccagacggg ttgcaatatt
    28201 gcctacgata ccgatcgaga aagtgcggat tttaaataaa tctaatgaaa ttaatgggtt
    28261 tggcgtgtgt cgtgcatgga gaatatagag aagcagtaac ccgatgctgg tgacaattac
    28321 cgtcaaggca atccagctgg cgacaatctt ttccccgaat agctctattc cgcttgagaa
    28381 gagaacaagg ctgaggccaa acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt
    28441 ggtgaaattg ggcatatgtt tgcgcgcgta aagaaggcct gcaataccta tggggatatt
    28501 gattaaaaat atccagtgcc aggttgccca ggtgaccagc acgccgccaa gaacggggcc
    28561 taaaattggc cccaccagac ccggcatggc gacaaaattc aatactggaa gaagttcatt
    28621 acgaggataa gcgcgcagta aggccagccg agcaacaggc atcatcattg cgccgcctat
    28681 cccctgaata acccggaaga caaccagctg tggtagcgaa ttagaaagtg cgcaggccag
    28741 agaacccaat gtgaacagac tcacggcaag ggtaaaaatg cgacgcgtac cgaagcgatc
    28801 ggctagccat ccgcttaccg gaataagcat cgccaccgtc agcgtataac tgatgatggc
    28861 tgattgcatc gcgagaggag aacgattaag gctatgagcg attgcgggta aggcggtatt
    28921 cagaatagtg gcatcaagtg cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc
    28981 cgccatactg cgcttctttt tatcgctcat tcaatgtcct gttatcgggt tatcacttat
    29041 caggtgagcg tagcagcgcc tgacaagctt taaatgccgc gtcgccatcg ctttggataa
    29101 tcgcatcgac tatcgcctga tgcagatcca gctttatcac tgtgtcgctg gtaattgacg
    29161 tgaagtaagt gtgataaacc gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg
    29221 cgctcatttc atagatatgc tcatgccagg ccatatcgac ttcgatccag cgttcacggc
    29281 gaaagttctc ttttaatgcc gccatttcgg ccattaacgt attgagatgc gctttctgtt
    29341 ccgcggtgcc aaccgttgct gccagtaggc aagcttgtgg ctccagacag atacgcataa
    29401 ccagaaagtg attgatgacc tgatgaaagt tctcttctgt catccaccag gtaagcaatt
    29461 cctgatcaag aaaattccag ttcgattgtg gcatgacccg ggtaccaatt cgcggtcgcg
    29521 gtaaaaccat cccttttgcc gttaacgttt tgaccgcttc gcgtaccgct gtacgactca
    29581 ctccaaattg ctcgcccagc tcaatctcac caggcaaaat ggtgccgggt tcatattcac
    29641 cttttaagat ccgttgcgcc agcttctcag ccagaacata cgaaaggttt ttctgtgcag
    29701 ctaactgttg tgcgcttaaa ggcattacta atcttccttt ttctttttat tcctccttag
    29761 tatgccacca ggaagtgtga ttacggttgc aaaaacggca aattgcttgt tttatggcac
    29821 atcaacgggg cttttgctga aaaaatgcgc ggtc
//
LOCUS       DAZFFG010000050        29769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFFG010000050 DAZFFG010000000
VERSION     DAZFFG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.050"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..424)
                     /locus_tag="ACHJ7J_002517"
     CDS             complement(<1..424)
                     /locus_tag="ACHJ7J_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5000738.1"
                     /translation="MQKYIFSADKNAFFPVELKIAYQESGEWPGDGIEIDDTVAAEFM
                     KEAPEGKYRGVIDGMPAWIDIPPPTHEEQIAAAELEKQQLINQVNEYINSKQWPGKAA
                     IGRLKGGELAQYNLWLDYLDALELVDTSGAPDIEWPTPP"
     gene            853..1419
                     /locus_tag="ACHJ7J_002518"
     CDS             853..1419
                     /locus_tag="ACHJ7J_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5000739.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDMLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     IQRIALIFDVGVSTIYRKFPANKINESP"
     gene            1562..2734
                     /locus_tag="ACHJ7J_002519"
     CDS             1562..2734
                     /locus_tag="ACHJ7J_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO5000740.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTIVVRVPQGETEDETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            2744..3259
                     /locus_tag="ACHJ7J_002520"
     CDS             2744..3259
                     /locus_tag="ACHJ7J_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO5000741.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            3314..3616
                     /locus_tag="ACHJ7J_002521"
     CDS             3314..3616
                     /locus_tag="ACHJ7J_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO5000742.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            3631..3750
                     /locus_tag="ACHJ7J_002522"
     CDS             3631..3750
                     /locus_tag="ACHJ7J_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO5000743.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            3743..6820
                     /locus_tag="ACHJ7J_002523"
     CDS             3743..6820
                     /locus_tag="ACHJ7J_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5000744.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKASAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLSSVTAAVTRTSSALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAAAGTVLRVLASGPLAMLRVALYAISGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPISAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAIVSRAATVPLV
                     QRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6817..7302
                     /locus_tag="ACHJ7J_002524"
     CDS             6817..7302
                     /locus_tag="ACHJ7J_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5000745.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PENDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTTTSALSDISKTVGGLL
                     S"
     gene            7299..8399
                     /locus_tag="ACHJ7J_002525"
     CDS             7299..8399
                     /locus_tag="ACHJ7J_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO5000746.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGKDITTVLDNRLMSLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQSLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSATGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDSE
                     WTITTLTHTVSPDNGFTTSLELEVKIDDFEME"
     gene            8490..8708
                     /locus_tag="ACHJ7J_002526"
     CDS             8490..8708
                     /locus_tag="ACHJ7J_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5000747.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(8944..10629)
                     /locus_tag="ACHJ7J_002527"
     CDS             complement(8944..10629)
                     /locus_tag="ACHJ7J_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO5000748.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10899..11276
                     /locus_tag="ACHJ7J_002528"
     CDS             10899..11276
                     /locus_tag="ACHJ7J_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO5000749.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(11306..11563)
                     /gene="grxA"
                     /locus_tag="ACHJ7J_002529"
     CDS             complement(11306..11563)
                     /gene="grxA"
                     /locus_tag="ACHJ7J_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO5000750.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            11723..12010
                     /locus_tag="ACHJ7J_002530"
     CDS             11723..12010
                     /locus_tag="ACHJ7J_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO5000751.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            11994..12716
                     /gene="nfsA"
                     /locus_tag="ACHJ7J_002531"
     CDS             11994..12716
                     /gene="nfsA"
                     /locus_tag="ACHJ7J_002531"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO5000752.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            12777..13679
                     /gene="rimK"
                     /locus_tag="ACHJ7J_002532"
     CDS             12777..13679
                     /gene="rimK"
                     /locus_tag="ACHJ7J_002532"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO5000753.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            13767..14243
                     /locus_tag="ACHJ7J_002533"
     CDS             13767..14243
                     /locus_tag="ACHJ7J_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO5000754.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            14594..15706
                     /gene="potF"
                     /locus_tag="ACHJ7J_002534"
     CDS             14594..15706
                     /gene="potF"
                     /locus_tag="ACHJ7J_002534"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO5000755.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            15801..16934
                     /gene="potG"
                     /locus_tag="ACHJ7J_002535"
     CDS             15801..16934
                     /gene="potG"
                     /locus_tag="ACHJ7J_002535"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO5000756.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            16944..17897
                     /gene="potH"
                     /locus_tag="ACHJ7J_002536"
     CDS             16944..17897
                     /gene="potH"
                     /locus_tag="ACHJ7J_002536"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO5000757.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            17894..18739
                     /gene="potI"
                     /locus_tag="ACHJ7J_002537"
     CDS             17894..18739
                     /gene="potI"
                     /locus_tag="ACHJ7J_002537"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO5000758.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            18799..19287
                     /locus_tag="ACHJ7J_002538"
     CDS             18799..19287
                     /locus_tag="ACHJ7J_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO5000759.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            19328..20455
                     /gene="rlmC"
                     /locus_tag="ACHJ7J_002539"
     CDS             19328..20455
                     /gene="rlmC"
                     /locus_tag="ACHJ7J_002539"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO5000760.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            20735..22078
                     /locus_tag="ACHJ7J_002540"
     CDS             20735..22078
                     /locus_tag="ACHJ7J_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO5000761.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            22100..22420
                     /locus_tag="ACHJ7J_002541"
     CDS             22100..22420
                     /locus_tag="ACHJ7J_002541"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5000762.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            22432..23913
                     /locus_tag="ACHJ7J_002542"
     CDS             22432..23913
                     /locus_tag="ACHJ7J_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO5000763.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWDPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHVNVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLVPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(23964..24695)
                     /gene="artJ"
                     /locus_tag="ACHJ7J_002543"
     CDS             complement(23964..24695)
                     /gene="artJ"
                     /locus_tag="ACHJ7J_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO5000764.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
     gene            complement(24986..25654)
                     /gene="artM"
                     /locus_tag="ACHJ7J_002544"
     CDS             complement(24986..25654)
                     /gene="artM"
                     /locus_tag="ACHJ7J_002544"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO5000765.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(25654..26370)
                     /gene="artQ"
                     /locus_tag="ACHJ7J_002545"
     CDS             complement(25654..26370)
                     /gene="artQ"
                     /locus_tag="ACHJ7J_002545"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO5000766.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(26377..27108)
                     /gene="artI"
                     /locus_tag="ACHJ7J_002546"
     CDS             complement(26377..27108)
                     /gene="artI"
                     /locus_tag="ACHJ7J_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO5000767.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(27126..27854)
                     /gene="artP"
                     /locus_tag="ACHJ7J_002547"
     CDS             complement(27126..27854)
                     /gene="artP"
                     /locus_tag="ACHJ7J_002547"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO5000768.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(28072..28587)
                     /locus_tag="ACHJ7J_002548"
     CDS             complement(28072..28587)
                     /locus_tag="ACHJ7J_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5000769.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            29211..>29769
                     /locus_tag="ACHJ7J_002549"
     CDS             29211..>29769
                     /locus_tag="ACHJ7J_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5000770.1"
                     /translation="MDYRFSWVIFNMINNISDQASSFPCTQLNQSDNFLDSLREFFAI
                     LNSSRKGELSTWDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFETD
                     VKKYLGKSEDNEVEIKAGNEIISIGIRNVSNGKLERQQFLFPLDYENKLQHQLDKHFT
                     IESHPLLYRHTMGSKIANVIFEKLYS"
BASE COUNT         7295 a         7152 c         7953 g         7369 t
ORIGIN      
        1 ccggaggcgt aggccattca atatctggcg caccggaagt atcgaccagt tccagtgcgt
       61 ccagataatc cagccacaaa ttatattgcg ccagttcccc acctttcagg cgaccaatcg
      121 ccgctttacc aggccattgc ttactgttta tgtattcgtt gacctgatta atcaattgct
      181 gcttttccag ttcggctgcg gcaatttgtt cctcatgagt tggcggtgga atatcaatcc
      241 atgcaggcat tccgtcgatg acacctctgt attttccttc tggtgcttcc ttcataaatt
      301 cggcggcaac agtgtcgtca atttcgattc catcaccggg ccattcgccg gattcctgat
      361 aagcgatttt aagctccaca gggaaaaacg catttttatc ggcactgaaa atatatttct
      421 gcatttctac cgtcctatcg aaatataact gaatctgtat tgctgtgaga tatcactggt
      481 tgccacacgc cacgctgaat tactgatatg ttcaaaattt acagataaaa ccagcggtgc
      541 aggattcgac ggttctgact gagcggcatc agacatgaca ctgactgaaa caagcggttg
      601 attagggaat ggtataggga agtgtccact gataatgcgg gtcgtgttcc ctgaaaaagt
      661 gccaaactga acaatatatc cacctggtag cctgaaccat cccgagccag aagcgaatgc
      721 tcccatatcc ggtatctgat tttcctctgt ccccacattc cttcttgccg cttctcccaa
      781 accaacgttt aagaaaatgc agaggtaaca gctaactggc atcatctccg gtttttattc
      841 agggggatga tcatgcttat tggctatgta cgcgtgtcaa caaatgacca gaacaccgat
      901 ttgcaacgta atgcactgaa ctgcgcggga tgtgagcgga tttttgagga caaaatcagt
      961 ggcactaagt ccgacagacc ggggctgaaa aaactactca ggacactatc ggcaggagac
     1021 acgctggttg tctggaagct ggacatgttg gggcgcagta tgcggcatct tgttacgctg
     1081 atagaagagt tgcgccagcg tggtgtgaat ttcagaagcc tgactgacag tattgatacc
     1141 agtaccccaa tgggccgttt cttttttcat gtcatgggtg ccctggctga aatggaacgc
     1201 gaactgatag ttgaacgtac cagggcaggg ctggctgcag ctcgtgccaa aggcagagta
     1261 ggtggacgcc gtcctaagtt gaccaccgaa cagtgggcac agattgggcg tttactcgag
     1321 gccggagaat caatacagcg tattgcactg atttttgatg taggcgtttc taccatttat
     1381 agaaaatttc cggcaaataa gatcaatgaa tccccctgaa tcagcattat gttgattatc
     1441 cctgcaagca gacaaatacc gttattttgt gtgaataacg acacaactgc gcttagctgt
     1501 ttgtcaggca caatcacttc aacatagggc gaagcctaat ccaatcagga ggttcgccac
     1561 tatggctcag gattaccacc acggggtgcg cgttgttgaa gtcaacgaag gcacccgatc
     1621 tattaccacg gtgagcaccg ccatcgtggg tatggtctgc acgggcgatg atgccgatgc
     1681 aaaaatgttt cctcttaata aacccgtgct gatcactgat gtgctgactg ccagcggtaa
     1741 agcgggtgag tcaggtactc tggcccgttc gctggatgcc atcgctgacc aggcaaaacc
     1801 cgtgaccatt gttgtgcgtg tgccgcaggg tgaaacggaa gacgaaacca cgaccaatat
     1861 catcggcgca gtgactgctg aaggtaaaaa aacaggtatg aaagccctgt tatctgccca
     1921 gtcacagctc ggcgttaaac cgcgcattct cggcgtgcca ggccacgaca ccaaggcggt
     1981 agctactgag ttgctgagcg tggcgcaaag cctgcgtgga tttgcttacc tgtcagcgta
     2041 tggctgcaag acagtgcagg aggcgatcac ttaccgcgaa aacttcagcc agcgcgaagg
     2101 gatgctgatc tggcctgact ttactggctg ggacacggtg ctgaatgccg aagcaacggc
     2161 atatgccacc gcccgtgcgc ttggtctgcg cgccaaaatt gacgagcaga ccggatggca
     2221 caaaagcctg tccaacgtgg gcgtgaacgg tgtcaccgga atttctgcag atgtgttctg
     2281 ggatctgcag gacccggcaa ccgatgcagg tctgctgaac cagaacgacg tcaccacgct
     2341 tgtgcgtaaa gacggtttcc gcttctgggg ttcccgctgc ctgagtgatg acccgctctt
     2401 tgccttcgaa aactacaccc gcacggcgca ggtgctgatg gacacgatgg cagaagcaca
     2461 catgtgggcg gtggataaac cgcttaaccc gtcgctggcc cgcgacatta tcgaaggtat
     2521 ccgcgccaaa atgcgcagcc tggtcagtca gggctatctc attggtggtg attgctggct
     2581 ggatgagtcg gtgaacgaca aagacacact gaaagccgga aaactcacca tcgactacga
     2641 ctacacgcca gtgccgccac ttgaaaacct gatgctgcgt cagcgcatca ccgatcagta
     2701 cctggtgaat ttcgccagcc aggtcagcgc gtaaggggac aacatggctt taccacgcaa
     2761 attaaaacac ctgaacctgt ttaacgacgg gaacaactgg caggggatcg ttgagtcgct
     2821 gacgctgccg aaatttaccc gcaaatatga gaagtatcgc ggcggcggaa tgccgggtgc
     2881 ggtggatgtg gatctggggc ttgatgacag tgcgctggac acagaatttt ccattggtgg
     2941 tactgaattg ctgctgttta aacagatggg taaagccacg gtggatggca tccagctgcg
     3001 ctttaccggc tctattcagc gtgacgatac cggggaagtg caggccgtgg agcttgtcgt
     3061 gcgtggacgt cacaaagaag tggattccgg cgagtggaag acgggcgaaa gcaacaccac
     3121 caaagtgacc agtaccaaca gctacgcgaa gctgaccatc aatggtgagg tgctctatga
     3181 agtggacctt atcaacatgg tggaaattgt ggacggtgtg gacctgatgg aagcgcaccg
     3241 caacgccctc ggcctctgat atatctgaac ggcgcgggat accgcgccag aacccaattg
     3301 acaggacagc aaaatgagcg ataagcagac tgaaaagacc attcaactgg atacccccat
     3361 caagcgcggt aaaacagaaa tcaccgaaat tgtgctgcgt aaaccgcagt ccggtgcgct
     3421 gcgcggtaca cgcctgcagg ccattatgga tatggatgta aacgcgatga tgaccgtgat
     3481 cccccgcatc tccagtccgg cactgactgc acaggaaatt gcagagatgg acccggcaga
     3541 tctcactgcc atgtcggttg aggttgtcac ttttttgttg aagaagtcgg tgcttgccgg
     3601 tttaccgaca gcctgacggt tgacgatctg gtggcagata tcgccaccat ttttcactgg
     3661 ccgccatccg ttactgacgt tatgccgctg accgaagtgc tggaatggcg gtataaagcg
     3721 attcagagaa gcggggccaa cgatgagtga taacaacctg cgtctgcagg tcattcttaa
     3781 tgcggttgac aagctcaccc gcccatttcg atctgcgcag gccagttcaa gagaactggc
     3841 tgctgctgtc aaaaaatccc gcgatgcaat aaagcagctt gatcaggccg ggagcagtct
     3901 ggacagcttc cgaaagctgc aggcagaaaa tcagaaatta ggcgacaggc tgaactatgc
     3961 ccgccagcgt gcaaatttgc tcagtcagga actgggagcg atggggccgc cttcgcaacg
     4021 tcaggttgtt gctctgggcc gtcaacggct ggctgttcag cgcctggaag aacgccagaa
     4081 aaagctgcag cagcagacgg cgcttgtgcg tgctgaactg taccgggcgg gaatttctgc
     4141 gaaagacgat gcgggagcaa ctgcccgttt agcccgtgaa acatcacgtt ataaccagga
     4201 actttcgaaa caggaggcgc ggctgaagcg actgggggaa gctcagcgca ggatgaatgc
     4261 agcgcgtgcc agttatgccc gttcgctgga ggtgcgtgat cgtattgcag gtgccggagc
     4321 caccaccacg gctgcagggc tggcaatggg tgcgccagtg atggcggcag taaaaagcta
     4381 taccagcatg gaagatgcca tgaaaggtgt ggcaaagcag gtcaatggtc tgcgtgacga
     4441 taatggcaac cgcactgcac gtttttatga aatgcaggat gccatcaagg ctgccagcga
     4501 acagttgccg atggaaaacg gtgcggtgga cttcgctgca ctggttgaag gtggtgcgcg
     4561 catgaacgtc gcaaaccctg acgacagctg ggaagatcag aaacgtgacc tgctggcctt
     4621 cgccagtacg gcagcaaagg cggcaacagc ctttgagctg ccagcggatg aactgtcaga
     4681 aagtctgggg aaaatcgccc agctctacaa aatccccacc cgcaatattg aacagctcgg
     4741 tgatgcgctg aactatctgg atgataacgc catgtcgaaa ggggcagaca tcattgatgt
     4801 gatgcaacgt ctgggcggtg tggctgaccg tctggattat cgtaaagcgg cggcgctggg
     4861 ttccaccttc ctgacactgg gcgctgcgcc agaggttgca gccagtgcag caaacgcgat
     4921 ggtgcgtgaa ttgtccattg ccaccatgca aagcaagagt ttctttgaag ggatgaatct
     4981 gctgaaactc aatcctgaag tgattgaaaa gcagatgacg aaggatgcga tgggaaccat
     5041 ccagcgcgtg ctggagaagg taaacgcgct gccgcaggac aagcgcctgt ctgccatgac
     5101 tatgttgttt ggtaaagagt ttggcgatga cgcggcgaaa ctggcaaaca acctgccgga
     5161 actgcagcgt cagttaaaac tgacagcggg caatgatgcg ctcggctcca tgcagaaaga
     5221 atccgacatt aacaaggatt cactttctgc gcagtggttg ctggtcaaaa ccggagcgca
     5281 gaacaccttc agcagcctgg gcgaaacgct gcgccagccg ctgatggata ttctgtacac
     5341 ggtgaaaagc gtcacggggg cgttgcgtcg ctgggtggaa gctaacccgg aactgacagg
     5401 cacactgatg aaagcatcgg ctgttgtggc tgcggttacc gtcggcctcg gcaccttagc
     5461 ggtggcgctg gctgcagtgc tggggccgct ggcagtgatc cgtctgggat tctctgtgtt
     5521 gggtatcaaa acgttatctt ccgttacggc agcagtaact cgaaccagca gcgcgttgtc
     5581 ctggctggct ggcgcaccac tggcactgct gcgacgcggg cttgcttcat cgggcaacgc
     5641 cgcaggttta cttactgcgc cgttgtcgtc tttgcgccgc acggcatcac tgacgggaaa
     5701 tgtcctgaaa actgtagcag gtgcgccggt tgcacttttg cggtctggat tatccggttt
     5761 acgtgctgtt gctgtgatgt ttatgaatcc tctggcggta ctgcgcggtg gactggccgc
     5821 cgcaggcacg gtgctgcgag tactggcatc tggtccactg gcgatgctgc gcgttgccct
     5881 gtatgccata tctggtctgt taggtgctct gctcagtccg ataggtcttg tggttactgc
     5941 actggcgggc gtggcgctgg ttgtctggaa atactggcaa cccatcaccg catttctcgg
     6001 tggcgtggtg gaaggattca aagcggcggc aggtcccatc agtgcagcgt tcgaaccgct
     6061 taagcccgtg ttccagtgga ttggcgacaa agtgcaggcg ctgtggggct ggtttactga
     6121 tctgctgacg cccgttaagt cgacctctgc cgaactgcag agcgcagcgg caatggggcg
     6181 gcgattcggg gaggcactgg cggaagggct gaatatggtc atgcatccgc tggactccct
     6241 gaaatccggc gtttcctggt tgctggagaa gctcggcatt gtcagtaaag aggccgcaaa
     6301 ggcaaaactg ccggaaagcg tgacgcgtca gcaacctgcg acggtgaatg cagacggtaa
     6361 agtgatgatg ccatcgggtg gttttccatc atggggatat ggctttgcgg ggatgtatga
     6421 cagcggcggg tatatcccgc gcgggcagtt tggcatcgtc ggtgaaaacg ggccggaaat
     6481 tgttaacggc ccggcaaatg tgaccagccg gagaaataca gctgcactgg ctgccgttgt
     6541 tgccggaatg atgggcgttg ctgccgcgcc agcagagctt ccaccgttgc accctttggc
     6601 acttcccgcg aaaggtggag aagcaattgt gagtcgcgca gccactgtgc cgctcgttca
     6661 acggattgag gcaccgacgc agatcatcat tcagacgcag ccaggacaaa gtgcgcagga
     6721 tattgcgcgg gaggtggcac gccagcttga tgaacgtgaa cgcaggctga aggcaaaagc
     6781 caggagtaac tacagcgatc aggggggata cgacgcatga tgatggtgct gggattgtac
     6841 gtgtttatgc tgcgcaccgt tccgtatcag gaactgcagt atcaacgcag ctggcgacat
     6901 gcggcaaaca gtcgggtaaa ccgacgtccg tccacgcagt ttctgggacc ggaaaacgac
     6961 atgctgacgc tttccggtgt tcttatgccg gaaataacag gcggcaggct gtcgttgctg
     7021 gcactggagc agatggcaga acaggggaaa gcatggcccc tgattgaagg cagcggcacg
     7081 atttacggca tgtatgtgat tgagggactg aatcagacta aaacggagtt tttccgcgat
     7141 ggtatgccgc gccggattga gttcaccctg tcgctcaaac gggtggatga atccctgtcc
     7201 gatatgttcg gtgatctcag tgcgcagctg aataatttgc aggatacgac aacgtctgcc
     7261 ttaagcgata tcagtaaaac ggtgggaggg ctgctgtcgt gaatttcagt tctgaactgc
     7321 ttaacaaagg caacaaaact ccggcattca gcatcagtat tgagggtaag gatatcacca
     7381 ctgtgctgga taaccgcctg atgagtctga cgctgacgga caatcggggc tttgaagcgg
     7441 accagcttga tctggagctg gacgacgccg acggaaaaat cgtgctgccg cgccgtggtg
     7501 cggtcattac gctggcgttg ggctggaagg ggcagtcgct tttcccgaaa ggagcattca
     7561 cggtggacga gattgaacac actggcgcac cggaccgcct gactatccgg gcgcgaagtg
     7621 ctgattttcg tgaaaccctg aatacccgcc gtgaaaaatc gtggcacaag accaccgttg
     7681 gggaagtggt gaaggaaata gctgcgcggc acaaactgaa gatggcattg ggtgaagacc
     7741 tgtcggataa acctgtggag catatagacc agaccaatga gagtgacggc agttttctga
     7801 tgcggctggc gcgccagtac ggtgctattg cgtcggtgaa aaatggcaat ctgttattca
     7861 tccggcaggg acagggcaaa agcgccactg gtaaaccact gccggtgatc actatcacac
     7921 gcaaggacgg cgacagtcac cgctttaccc tagcagatcg cggagcctac acgggggtaa
     7981 ttgccagctg gttgcatacc cgcgaacccg cgaagaaaga aagcactacg gtgaagcgta
     8041 agcgcaggac taagaagcag aagaaagagc cggaagcgaa gcagggcgat tacctggtgg
     8101 ggacggatga aaacgtgctg gtacttaatc gcacttatgc caaccggagc aacgccgaac
     8161 gagcggcaaa aatgcagtgg gaacgcctgc aacgcggcgt tgcgtcattc tcgctacaac
     8221 tggcggaagg gcgggcagat ctctacacgg aaatgcctgt gaaggtcagt ggttttaaac
     8281 agccgataga tgattcggaa tggaccatta cgactctgac acataccgtc agcccggata
     8341 acggttttac gaccagtctg gagcttgaag tgaagattga tgatttcgaa atggaatgat
     8401 tcttcgcaat ggagaacttt taagttttca aaatggaata atgcggtatc attattgtga
     8461 atttagcaaa aatggggaga actcgaaaaa tgatgatttg cccactgtgt ggaagtgccg
     8521 cccatactcg cagcagtttt caggtatctt cattgaccaa agagcgttac aaccagtgcc
     8581 agaacattaa ctgcagccat acttttgtta cccatgaaac ttttgttcgt tcgattgcaa
     8641 cgccaaaaga gtcaaatccg gttcagccgc atccaatgaa atcaggacag gtggcgctct
     8701 ctctttgacg ctgccgccaa tttgtcgcca tcgttaaaaa acagtgcttc taacatcatg
     8761 attttaaaag gcataaattt caggcaacaa aaaacccatc aaccttgaac cgaaatggcg
     8821 gggttgatgg gctccacaaa atggggacat caaagaaaag cagtggcact aattaagact
     8881 gatgccctgc ggaaaagttc tgcggttgtg caaaaaaatt tcattttcag ggcaacttca
     8941 gttttatcct aatcctggcc ataccatgac gatgattgtc cctgccagcg tcagcaggac
     9001 gttggcgatt gcataggtgc ccgcatagcc cagcgccggg atgttactgc gagctgtatc
     9061 actgatgatc tccattgccg gcgcgcaggt gcgtgcgccc atcattgcgc cgaacaacag
     9121 tgcgcggttc attcgcaata cataagcacc gaacaagaaa cagataacca cgggcaccag
     9181 actgacaatc aatccggcaa tcaacatctg gccgccaatc gcgcccaggc cgttattaat
     9241 accgctaccg gcgctcagac caacgcctgc cataaacacc atcaagccga actctttcac
     9301 catgcttaat gcaccttgcg gaatgtaacc gaaggtcggg tggttagcac gcataaagcc
     9361 cagcataatt ccggcgaata acaacccggc agcgttcccc atgccgaaac tgaatgtgct
     9421 gaactggaag gtgatcatcc cgatcatcag cccaataaca aagaaggcgc agaatgccag
     9481 caggtcagtg acctggctgt gaatcgagat aaagccgatg cgatcggcga tggtttttac
     9541 gcggcgggca tcgccgctga cttgtaaaac gtcacctttg ttaagcacga cgttgtcatc
     9601 tatcggcatc tcaatctggc tacgaataac gcggttaagg aagcaaccgt gatcggtcaa
     9661 cttcagttgt gcgagacgtt tgccaacggc gttatggttt ttaacgacca cttcttcagt
     9721 gacgatacgc atatcgagaa ggtcacgatc gaaaacttct ttgccgttac ggaagctggg
     9781 atcgagtcgg gcatgggcgt cgggatagcc taccaacgct atttcatcgc ccatttgtag
     9841 cacggcatca ccgtctggat ttgccagaat cccgttacgt cgaatacgtt caatgtagca
     9901 gccggtttgt cgataaatac ccagttcacg cagatttttg ccgtcggtcc aggccaccag
     9961 ctccgggccg acgcgatagg cgcggatcac cggtaaataa accttacggt tggcatcagt
    10021 gtccaggcca cgttcgcggg cgatttgctg ggcgctggtc tgtaagtcct gatgctgcaa
    10081 tttcggcaag taacgcgcac caacaatcaa actcaccaga ccgattaaat aggttaaggc
    10141 atacccgagg ctcagattat ccagtgccag tgagagctgc ctgctttcca tgccggaatg
    10201 acgcagtgta tcgccagcac cgaccagaac cggtgtcgac gtcatagagc ctgctaacat
    10261 accggccgtc aggccaatat cccagccaaa cagcttacct aaccctaagg cgatcaccag
    10321 cgcactgcca accatcacca gtgctaacat taggtaattt ttcccatcgc gaaaaaaaat
    10381 ggaaaaaaag ttcggtccgg cttcgacccc gacgcagaaa ataaacagca taaagccaag
    10441 attaagcgca tcggtgttaa tgctgaaatg ttgttggcct aataacagcg atacgactaa
    10501 aacgccaatg gaattaccca gttggatcga accaagtcgt aactttccga gacatagccc
    10561 aagcgcgagg accacaaata ataacagaat gtaattccca tttaacaatt cggcgacgtt
    10621 tatattcacg gaggctaact tcttgtttac tagtaagctg ttgaaagaaa tggtaattta
    10681 cgataatgtt ttttaccaga attcagggcg cagattcatt cagcgcacct aaacgatagt
    10741 aaagtatcaa tatattttac tagtgtaatc acattaggta tcaacggcta tatgaattgc
    10801 gttggcctat attagcatgg aatgcgaagc ggctttatct tactgaacgc cacactggcg
    10861 aaaaatgtgt tcgatagacg cagtgtcagg aggaacgagt gaaacataaa caacgttggg
    10921 cgggggcaat ctgctgtttt gtcctcttca ttgtggtgtg cctttttctg gcgacgcaca
    10981 tgaaaggcgc ttttcgggct gccgggcatc ctgaaatcgg cttgctgttt ttcattcttc
    11041 ctggagcagt tgccagtttc ttttcacagc gtagagaagt cctgaaacct ctatttggcg
    11101 caatgctggc ggcaccctgt tcaatgctta ttatgcggct gtttttttca ccgacccgct
    11161 cattctggca agagctggca tggttactaa gcgcggtgtt ctggtgtgcg ctgggggcac
    11221 tgtgtttctt atttatcagt agcttgttta aaccacagca cagaaaaaat cagtaaagcc
    11281 ctcaacgcga gggcttgtca gacgatcagg cgtccagatt ctctttcacc catgcagcaa
    11341 aatcggtata gccgccgata tgttgctgat cgacaaaaat ctgcggcacg gtttctacgg
    11401 gtttacctgc cttttgttgt agatcttctt tagtgatccc ttccgcacga atatctacat
    11461 actgatactg aaaatcatcg cgttcattgc tcaatttctc agccagatct tttgcacgca
    11521 cacagtaagg gcaacccggg cgaccaaaaa taacggtttg cattatttct ctcctcatag
    11581 atttatgcct gtaatgatca cgctaaaatg tattcgctga aagtaggttt aacctgttgc
    11641 attaattgct aaaagctata actgttaaac acaatacagt gaaaagtttt agactgaagg
    11701 ctcactttgc agagggaagc gtatgcgcgc gatcggtaaa ttgcctaaaa gcgtgttgat
    11761 actggaattt atcggaatga tgttgctggc ggtggcgctg ctgtcggtaa gcgactccct
    11821 gtcgctgcct gagccatttt ctcggccaga agtgcagatt ctgatgattt ttctcggtgt
    11881 tttgctcatg cttcccgctg cggtggtggt tattcttcag gtggcaaaac gtcttgcccc
    11941 acagctgatg aaccgtccac cgcaatattc acgttcagaa agagaaaaag ataatgacgc
    12001 caaccattga acttatttgt ggccatcgct ccattcgcca tttcactgat gaacccattt
    12061 ccgaagcgca gcgtgaggcg attattaaca gcgcccgtgc gacgtccagt tccagttttt
    12121 tgcagtgtag tagcattatt cgcattaccg acaaagcatt acgtgaagag ctggtgacgc
    12181 tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt ctgggtgttc tgtgccgact
    12241 ttaaccgcca tttacagatc tgtccggatg ctcagctcgg cctggcggaa cagctgttgc
    12301 tcggtgtcgt tgatacggca atgatggcgc agaatgcatt aaccgcagcg gaatcgctgg
    12361 gattgggcgg cgtatatatc ggcggcctgc gcaataatat tgaagcggtg acgaaactgc
    12421 tgaaattacc gcagcatgtt ctgccgctgt ttgggctgtg ccttggctgg cctgcggata
    12481 atccggatct caagccgcgt ttaccggcct ccattttggt gcatgaaaac agctatcaac
    12541 cgctggataa agacgcactg gcgcagtatg acgagcaact ggcggaatat tacctcaccc
    12601 gtggcagcaa taatcgccgg gatacctgga gcgatcatat ccgccgaaca atcattaaag
    12661 aaagccgccc atttattctg gattatttgc acaaacaggg ttgggcgacg cgctaaaacc
    12721 gccacgtcga tgtatgatac gcgggctttt gaccaggtct gacagagagg tgcagggtga
    12781 aaattgccat attgtcccgg gatggaacgc tctattcgtg taagcggctg cgtgaagccg
    12841 ctatacagcg cggtcacctg gttgaaattc ttgatccgct ttcttgctac atgaacataa
    12901 atcctgcggc gtcttctatt cactacaaag gccgcaagtt accccatttt gacgcagtga
    12961 tcccgcgtat tggcaccgcc attacctttt atgggacggc ggcactgcgc cagttcgaga
    13021 tgctggggag ctatccgctc aatgagtcgg tcgccattgc ccgggcgcgt gacaaattgc
    13081 gttccatgca actgctggcg cgtcagggca tcgacctgcc tgtcacgggc attgcgcatt
    13141 cgccggatga taccagcgat ttaatcgaca tggtcggtgg tgcgcctctg gtggtcaagt
    13201 tggttgaagg cacgcaggga attggcgtcg tgctggcgga gacacgtcag gcggcggaaa
    13261 gcgtgattga cgctttccgc ggtctgaacg cgcatattct ggtgcaggaa tatatcaaag
    13321 aggcgcaagg gtgcgatatc cgctgtctgg ttgttggcga tgaagtggtc gctgcgattg
    13381 aacggcgggc gaaagagggc gattttcgtt ccaatttgca tcgtggcggc gcggcaagcg
    13441 tcgccagtat cacaccacag gagcgtgaaa tcgcgataaa agccgcgcga acgatggcgc
    13501 tggacgttgc tggtgtggat attctgcgtg ctaatcgcgg gccgttggtg atggaagtga
    13561 atgcgtcgcc ggggctggaa ggaatagaaa aaaccaccgg tatcgacatc gcgggtaaaa
    13621 tgatccgctg gatcgaacgc cacgctacga cagaatattg cctgaaaacg ggtggttagt
    13681 cgcaatcaca ttactgatca tggttttgcc tgcgcttttt gcgtaagctg tgccggtctt
    13741 tttatcgaaa gaggttgtac aaaattatga catcgctggt cgttcctggt ctggatacgc
    13801 tgcgtcaatg gctcgatgac ctggggatga gtttttttga atgtgataac tgtcaggctc
    13861 tgcatctgcc ccatatgcag aatttcgacg gtgtctttga tgccaaaatc gatctgatcg
    13921 ataacacgat cctgttttct gccatggcgg aagtccgacc ttcagccgta ttgccgctgg
    13981 cggcggattt atctgccatc aatgccagtt cgctgaccgt gaaagcattt cttgatatgc
    14041 aggatgataa tctgccaaag ctggtggttt gccagtcttt atccgttatg cagggcgtaa
    14101 cctatgagca gtttgcatgg ttcgtgcgtc agagcgaaga gcagatttcg atggtcattc
    14161 ttgaagctaa tgcccatcaa ctgctgttac cgactgacga tgaagggcaa aacaacgtta
    14221 ccgaaaacta tttcctccac tgataactcc tttcgagcac gcagtcgctg gtgcagtggc
    14281 tgcgcgctgc aaaattatct gctgttttta accttttctt aaagattatt tcacttctct
    14341 tgtgtcgatt tggctttatc acatagagca aatacgcata aaaatttgtt aaataccgtt
    14401 ttttaatccg agctatagtc tcaaaccctg gctaaagtta ttcttgcgat gcttttatat
    14461 agcgagcagt gctggccggg agagagttct cttttcttac accgcgccga taaaaaatat
    14521 gcacgtttat tgcatatctt tcagtgtgac aacttttgtt cgtttgttaa cgaactttca
    14581 gaaggaaaga gatatgaccg ccttaaataa aaaatggctt tcgggtctgg ttgcgggagc
    14641 tctgatggcc gtctctgtcg gcacgctcgc ggctgaacaa aaaacactcc acatttataa
    14701 ctggtctgat tatatcgccc ccgacacggt ggctaacttt gaaaaagaaa ccggtattaa
    14761 agtcgtctac gatgttttcg actctaacga agtactggaa ggcaaattaa tggccgggag
    14821 caccggcttt gacctggtgg tgccgtcagc aagctttctt gaacgccagc tgacagcggg
    14881 agttttccag ccgctggata aaagcaaatt gccggagtgg aagaatctcg acccggaact
    14941 gctgaagttg gtcgccaaac acgatcccga caataaattt gctatgccct atatgtgggc
    15001 gacgaccggg attggctata acgtcgataa agtcaaagcg gtgctgggcg aaaacgcgcc
    15061 cgtcgatagc tgggacttga tcctcaagcc tgaaaatctg gaaaaactga aaagctgcgg
    15121 tgtatctttc ctcgatgcgc cagaagaagt ttttgctacc gtgctgaatt atctcggcaa
    15181 agatcccaac agcactaaag cggatgatta caccggaccg gctaccgatc tgctgttaaa
    15241 gctgcgcccg aacattcgtt atttccattc atctcaatac attaacgacc tggcaaacgg
    15301 cgatatttgc gtcgctatcg gctgggcagg tgatgtctgg caggcgtcaa accgcgcgaa
    15361 ggaagcgaag aatggcgtga atgtctcgtt ctcgattcca aaagaagggg cgatggcgtt
    15421 ctttgatgta ttcgccatgc ctgcggatgc caaaaataaa gacgaagcct atcagttcct
    15481 gaattacctg ctgcgtcctg atgtggtggc acatatctct gaccatgtgt tctatgctaa
    15541 cgccaataaa gcagccacgc cgctggtgag tgcggaagtc cgtgataacc cgggtattta
    15601 tccgcctgcg gatgtccgcg cgaagttgtt cactctgaaa gtacaggatc cgaaaatcga
    15661 ccgtgtgcgc acccgcgcgt ggaccaaagt gaagagcgga aaataatccg cagccttaga
    15721 tgccgggcgg gagcgccact ccctccggca attcgcacca acatggtgcg cttgcacaca
    15781 tttaatgccg gagagcagcc gtgaatgacg ctatccctcg cccccaggcg aaaacccgta
    15841 aggcgctgac cccgctatta gaaatccgca atctgaccaa atcctacgat ggtcaacatg
    15901 cggtggatga tgtcagcctg accatctaca aaggtgaaat cttcgcgctg ctgggcgcat
    15961 ccggctgtgg caagtccacg ctgctgcgta tgctggcagg tttcgaacaa ccttctgccg
    16021 gacagataat gctcgatggc gtcgatttgt cacaggttcc gccttacctg cgccccatca
    16081 atatgatgtt tcagtcttac gcgctgtttc cgcatatgac cgtggaacag aacatcgctt
    16141 ttggcctgaa acaggacaaa ctaccgaaag cggaaattgc cagccgggtc aatgagatgc
    16201 tcggtctggt gcatatgcag gagttcgcca aacgcaaacc gcatcagctt tccggtggtc
    16261 agcgacaacg tgtggccctg gcccgaagcc ttgccaagcg cccgaaacta ttactgctcg
    16321 acgagccgat gggcgcgctg gataaaaagc tgcgtgaccg gatgcagctt gaagtggtgg
    16381 atattctgga gcgcgtcggt gtgacttgtg tgatggtcac ccacgatcag gaagaggcga
    16441 tgaccatggc ggggcgtatc gccattatga atcgtgggaa atttgtccag attggcgaac
    16501 cggaagagat ctacgagcat ccgactaccc gctatagcgc cgaatttatt ggctcggtta
    16561 atgtctttga aggtgtactc aaagagcgtc aggaagatgg tctggtgctt tattcgccgg
    16621 ggctggtgca tccactgaaa gtcgatgcgg atgcttcagt ggtcgataac gtgccggtac
    16681 acgtagcgct gcgtccggaa aaaatcatgc tttgcgaaga gccgcccgcc aatggttgta
    16741 acttcgcggt gggggaggtg atacacattg cctatctcgg cgatctttcg gtgtatcacg
    16801 ttcgtctgaa aagtgggcag atgattagcg cccagctaca aaacgcccat cgtcatcgta
    16861 aagggttacc gacctggggc gacgaagtgc gtttgtgctg ggaagtggac agctgtgtgg
    16921 tgcttacggt ttaaggagca aagatgagta cacttgaacc tgctgcccag tcgaaaccgc
    16981 cgggcggatt taagctgtgg ttgtcgcagc tgcaaatgaa gcatgggcgc aaactggtca
    17041 ttgcgttgcc gtatatctgg ttgatcttgc tgtttctgct gccatttctg attgtcttta
    17101 aaataagcct ggcagagatg gcgcgcgcta ttccacctta taccgaactg atggagtggg
    17161 ctgacgggca actttccatc actcttaatc tcggtaattt tctgcaactg accgacgatc
    17221 cgctctattt cgatgcttat ctccagtcgt tacaggtggc ggcgatttcg acattttgct
    17281 gtttactgat cggctatccg ctggcgtggg cggtggcgca cagtaagcct tcgacccgta
    17341 atatcttatt attactggtg atcctgccgt cgtggacctc gtttctgatt cgtgtctacg
    17401 cctggatggg aatattaaaa aacaacggtg tgctgaataa ttttctgctg tggctggggg
    17461 ttatcgatca accgctgacc attcttcata ccaatctggc agtttatatt ggcattgttt
    17521 acgcctacgt gccgtttatg gtgctgccga tttataccgc gttgattcgt attgattatt
    17581 cgctggtgga agcagcgctg gatctcggtg cacgaccgct gaaaacgttc tttactgtga
    17641 tcgtgccgct gactaaaggt gggattattg ccggatcgat gctggtgttt atcccggctg
    17701 tgggcgagtt tgtgatcccg gaattgctcg gtggcccgga cagcatcatg atcgggcgcg
    17761 tgctatggca agagtttttc aataaccgcg actggccggt ggcctcggcg gtagcgatca
    17821 tcatgttgct gctgctaatt gtgccgataa tgtggttcca caaacaccag caaaaaagcg
    17881 tgggagaaca cggatgaata atttaccggt agttcgttcg ccctggcgga ttgtgatttt
    17941 gctgctgggc tttacctttc tctacgcgcc aatgctgatg ctggttatct attcgtttaa
    18001 cagctcgaag ctggtgacgg tgtgggccgg ctggtcaacg cgctggtatg gcgaattatt
    18061 gcgcgatgac gcgatgatga gtgcggttgg tttaagtctg acaattgcgg cctgtgcggc
    18121 gacggcggcg gcgatcctcg gaactattgc ggcggtggtg ctggtgcgct ttggcaggtt
    18181 tcgcggatca aatggctttg cctttatgat caccgcgccg ctggtaatgc cagatgtcat
    18241 cacgggcttg tcgctgttgt tattattcgt cgcgcttgct catgccattg gctggcctgc
    18301 ggaccgcggt atgcttacca tctggctggc gcatgtcact ttctgtacgg cttatgtggc
    18361 agtcgttatt tcgtcgcgtc tgcgggaact ggatcgctcg atagaagaag cagcgatgga
    18421 tctcggtgcg acgccgctga aagtgttttt cgtcattacg ctaccgatga tcatgcccgc
    18481 gatcatttct ggctggttac tggcttttac tttgtcgctt gatgatctgg tgatcgccag
    18541 ctttgtttcc ggaccgggag ccaccacgtt accgatgctg gtcttttcca gcgtgcggat
    18601 gggggtgaat ccggaaatca acgccctggc aacattgatc ctcggtgcgg tcggaattgt
    18661 cggatttatc gcctggtatc tgatggctcg cgcagaaaaa cagcggatac gcgacatcca
    18721 gcgtgcaaga cgtggctgaa gacactaaaa tttgccaacc tggctacata atgccgcgta
    18781 tgtcgcggca ttgttttcat ggaagacgaa acgttgggat tttttaagaa aacatcttca
    18841 tctcatgctc gcctgaatgt gcctgcgctt gtgcaggtgg cggcgctcgc cattattatg
    18901 atccgtggcc tcgacgtgct gatgattttc aatacgctgg gcgtgcgcgg tattggcgag
    18961 ttcattcatc gcagcgtaca aacctggagt ttaacgctgg tctttttaag cagtctggtg
    19021 ctggttttca ttgagatctg gtgtgcgttt tcactggtga aagggcgtcg ctgggcgcgc
    19081 tggctatatc tgctgacaca aatcaccgcc gcaagttact tgtgggcggc ttcgctgggg
    19141 tacggttatc cggagctgtt cagcattccc ggtgaatcaa aacgtgaaat cttccatagc
    19201 ctgatgctgc agaagctgcc ggatatgctc atcctgatgc tgctgttcgt tccctcgacc
    19261 agtcggcggt tcttccagtt gcaataatgt gtataatcgt cgcccctgat gatgtgaagg
    19321 tcaatgtatg cagtgcgcac tttacgacgc gggtcgctgt cgttcctgtc agtggataac
    19381 gcagccgatt ccagagcaac tctccgctaa aaccgccgat cttaaaaatc tgctcgccga
    19441 ttttccggtt gaggaatggt gcgcgccggt gtcaggccca gaacaagggt ttcgtaataa
    19501 agccaaaatg gtggtgagtg gtagcgttga aaaaaccctg ctcggtatgc tgcatcgcga
    19561 cggcacaccg gaagaccttt gtgactgccc gctttatcct gcctcatttg cgcccgtttt
    19621 tgcggcgcta aaaccgttta tcgcccgagc ggggttaacg ccctacaacg tggcgcgtaa
    19681 gcgtggcgaa ctgaaataca ttctgctgac cgaaagccag agcgatggcg gcatgatgct
    19741 gcgctttgta ctgcgttctg ataccaagct ggcgcaactg cgtaaggcgc tgccgtggtt
    19801 acacgaacaa ctaccgcagc tgaaagttat taccgtcaat attcagccgg tacatatggc
    19861 gattatggaa ggggagacgg agatctacct gaccgaacaa caggcgctgg cagagcgttt
    19921 taacgatgtg ccgctgtgga tccgtccgca aagtttcttc cagactaatc cggcggtcgc
    19981 cagccagctg tacgctaccg cgcgcgactg ggtacgacag ctgccggtta aacatatgtg
    20041 ggatctgttc tgcggtgtgg ggggctttgg tttacactgc gcgacgcctg acatgcagtt
    20101 aaccgggatc gaaattgcgc cagaggccat tgcctgtgca aagcagtcag ctgctgaact
    20161 gggcttaacg cgtttgcaat ttcaggcgct ggactccact cagtttgcca ccgctcaggg
    20221 ggaggtgccg gagctggtgc tggttaaccc gccgcgccgc ggcattggta aaccgctgtg
    20281 tgattatctc tcaacgatgg caccgcgttt tatcatctac tccagctgta acgcccaaac
    20341 catggcgaaa gatatccgcg aactgcctgg ttatcgcatt gaacgggtgc agcttttcga
    20401 tatgttcccg cacaccgcgc actatgaagt gctgacgctg ctggtaaaaa tgtaaataca
    20461 tcattcaaat caatatcacc tcggcataca tccatccaaa ataataatcg tgatcgatcg
    20521 attaataata accgatcgat cacggttata tattgctcta tttttgcaaa tcaaatcaca
    20581 aataatcaaa taagatcttt aattgaactg ttttgattac tacagtgcac ttaccgaata
    20641 catacagagg cattgcttag cgaaacacaa aatttcctga gacattatta acgtcagcag
    20701 aaggttcatg cattgacaac ggaaatgtac gcttatggag ggcgttccga cgatgtttgc
    20761 caaattcata gatgttattc aaacgttttt aactgaaccg gcaattttaa ttggtttgct
    20821 ggtgggtatt ggttatgccc ttgataagaa atcgccaata aaaattataa ccggcatggt
    20881 cagcgccatg gtgggattaa tgatggtgtt atttggtggt ttccagtttt ccgcaacatt
    20941 taaaccggta gcagaagccg taagtaaagc ctatggtgtt cacggatatt taatggattc
    21001 ctacgcaatg aaagcggcaa cgcaaattgc gttgggggat aattttggtt atgtcgggta
    21061 tgtatttgta ctggcgtttt ttaccaacct gctgctggta ttatttggtc ggtatactgg
    21121 tgccaaaggg atatttttga cagggaatac cggtgtttca cattctcagg cggtattatg
    21181 gttgattgtc ttttggctgg gttttagctg gactacatcc attattattg ccggaatatt
    21241 aacgggtgta ttctgggcgt tctccaccac gctcattgtt aagcccattg ccaaagttac
    21301 caaagatgcc ggttttacca ttgcccataa ccagatgttg ggattgtggt ttttctccaa
    21361 attcgcccat aaatttggtg accctgaaaa gcacgatgct gaaaacctga aacttcccgg
    21421 gtggctggcg atttttaacc ataacgtaac ggctatcgcc attgtgatga cgctgtttgt
    21481 cggtggtttc ttgctatcaa cgggtatcga taatgtccag ttaatggcga aaggcaagcc
    21541 ctggtacatc tatatcatca accttggctt acagttctcc atgtatatgg tcattctgct
    21601 gcagggtgtg cgcatgatgg tgggcgaaat taacggttcg tttaaaggct ggcaagatcg
    21661 ctttattcct aacgctattc ctgctgtcga tgttgcagcg ctgttacctt tttcacccaa
    21721 tgccgcaacg ttaggcttcg tcttctgtac ttttggcacc attttttcga tggggatctt
    21781 attactggtt cacagcccaa ttatggtatt gcctggcttt gtacctctgt ttttctctgg
    21841 cggtccaatt ggcgtattag caaaccgcat gggcggatac cgttccgtaa ttatctgtac
    21901 gttcttgctg ggtattattc agaccttcgg tacggtgtgg gctattccgt taaccgggct
    21961 tgctgaaaat ggcgtcggct ggacaggaat atttgactgg gcaaccgtat ggccagctat
    22021 ttgtgaagtt ctgaaattta tcgctgcaac attccatctt ggtccttacg cgggttaatt
    22081 aaatattaag ggtttaaaaa tgaaagatac aattaatatt ctgtttgtct gtggttacgg
    22141 tgtaggcagt agcgttatgt tacaaacggt cgttaaaaaa gcgctgacca aatatgattt
    22201 caaagttgaa atggaacata ccgccgcagg tgaagtcggc ggatttaccg actgggcgga
    22261 tatttttgct atttccaaaa aattactcga tgtggttagt cttgacccga aacaagggca
    22321 atatttgatt ccgatagaga atattatgga cggtgaaact attggtcagc aaatttatga
    22381 agtggttcag caacattttc cgcatttaat taatcaataa ggtagggcgt aatgagagcg
    22441 attattctgc tgttcgatag tctgaataaa cgttatctgc caccttatgg cgatgcgtta
    22501 accaaagcgc ctaatttcca acgtctggcg gctcatgccg ccacctttga aaacagttac
    22561 gtcggcagta tgccgtgtat gcctgcccgg cgggagttgc ataccgggcg atgtaacttc
    22621 ctgcatcggg agtgggggcc gttagaaccg tttgacgatt ccatgccgga gctgcttaaa
    22681 aaggcgggga tctataccca cctgataagc gatcatttgc attactggga agatggcggc
    22741 gggaattatc ataatcgcta cagttcgtgg gagattgtgc gcggacagga aggcgatcac
    22801 tggcatgcaa gcgttgcgca accgcctatt cccgaggtgc tacgggtgcc gcaaaagcag
    22861 accggtggcg gtgtttctgg tctgtggcgc catgactggg caaaccggga atatattcag
    22921 caggaagcgg attttccaca aactaaagtg tttgatgcag gctgtgcctt tatccacaaa
    22981 aatcacgcgg aagataactg gttattgcag atcgaaacgt ttgatccgca cgagccgttt
    23041 cacacaacgg aagagtatct ttccttgtat gaagataact gggatggacc gcattatgac
    23101 tggccgcgtg gccgggtgca ggaaagcgac gaggccgtgg agcacatccg ttgccggtat
    23161 cgttcgctgg tgtcgatgtg cgatcgcaac ctggggcgca ttctcgacct gatggatgag
    23221 cacgatctgt ggcgagatac catgctgatt gtcggcactg accacggctt tttactcggt
    23281 gagcatggct ggtgggcgaa aaaccagatg ccttattaca acgaagttgc caataatccg
    23341 ttgtttatct gggacccgcg cagtggtgta aaaggagagc ggcgacaggc actggtacaa
    23401 atgatcgact gggcgccaac gttgtatgac ttttttcaac agccagtgcc gcccgatgtg
    23461 caggggcaac cgctggcgaa aacggtcagt cacgatgaac cagtacgcag ctcggcgatg
    23521 tttggtgtct tcagtggtca tgttaacgta actgatgggc gttatgtgta tatgcgtgca
    23581 gcgctgccgg ggcgtgagga tgatattgcc aactacacgt tgatgtcctg caaaatgaac
    23641 agccgctatc cggtggatga gatgcgggct ttatcgctgg tcccaccgtt tcgttttacc
    23701 aaagggttac aggtattacg catcccggca caggaaaaat ataaggggtt gaatcagttt
    23761 ggtcatttgc tgtttgatct gcaaaacgat ccgcagcagc tacatccgat tcatgatgat
    23821 gtgatcgagt cccggatgat cgcgttgctg attcagttga tgaaagataa tgatgcgccg
    23881 ccagagcagt ttcagcgcct gggattagcg tagagaagtg attttgccgg agtgggtgtt
    23941 cgcccactcc ggcaaatgaa taattattgc gggaaccact gatcgctgat ttgttgatat
    24001 gtaccgtcag ctttaattgc tgccagcgcg ttattcagtt tttccagcag agctttgtta
    24061 tccggacgta cagcgatacc caggccggtg ccgaagtatt gcggatcggt cactttttca
    24121 gtcgcaacgc ccagttgcgg gttggttttc agccattcat taaccaccgc tgtgtcacca
    24181 aataccccat caatacggcc atttttcaga tcgataaagg cattctgata actgtcatag
    24241 gagacagttt tcacttccgg gtgctgatcc tgaatatatt tctggtgcgt agtaccgttt
    24301 tccatcccaa tacgtttgcc tttcagatcg gcaaacgttt tgtaggtatc ttttttggca
    24361 atcacgacgg ctgagttttc atagtagggc gtggtaaacg atacctgttt gctacgctcc
    24421 ggggtgatat ccataccgga gattacggcg tcatattttc tgaatttcag tgacgggatc
    24481 aggctgtcga acgcgtgatt agtaaaagta cattctgcct gcatttgttt gcacaaggct
    24541 tttgccagat cgatatcaaa gccgacaatc tcattattag cacctataga ttcaaagggt
    24601 ggataggtgg ctgaaacgcc aaaattgatt ttctctgcgg cagaagcacc gaaagtaaag
    24661 gaagcaagta aagcggcaag aactaacttt ttcatgatgg aactcccgtc tgtcaatctt
    24721 atgattattg gccgtgtctg cggcatggga taacaatgcc accaagtgaa tttatatgca
    24781 ataaatatga ttaaataatt tgcatgaaat aaaaaaagac ggacaactca ctaagttgtc
    24841 cgtctttatt ataagaattt atgcactatg taggccggat aagacatcca cgccgcatcc
    24901 ggcacaggca ccgtgctgat gtctgatgcg acgctggcgc gtcttatcag acctacaaac
    24961 cccccggcga atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct
    25021 cgatcagacg catcatcagc gtcagcaggc cgttaacgac caggtaaata atccctgccg
    25081 caccgaacac cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt
    25141 ccatcagcgt aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg
    25201 aataagaaga gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt
    25261 tgctcattcc cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac
    25321 cgtaaaacag ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata
    25381 accacggttc tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc
    25441 ccggcccgta ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga
    25501 tataaccccg caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa
    25561 tcaatgccag aatcagcgcc acaatcagcg aggcaaccgt cagcgtcagg ctggtgtgca
    25621 gccctttcat cagttcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt
    25681 cgcgcgcagg tcaatgcgtt tgagaatgta ctgactcaac agggtgatca ccaggtaaat
    25741 cgccgccgcc acaatgtacc aggtaaatgg ttcctgggta cgggtagcga tgcttttggt
    25801 ttgtagcatt aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac
    25861 cagccactgg ttaccgaggc caggcaacgc atgacgccac atctgcggca tcaccagacg
    25921 gaaaaagata gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg
    25981 caccgctttc agcgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagcgacag
    26041 agcgatgaca ccacaaagga acgggctaac gtcgaagttc tcgatgtcca tctgcaccgg
    26101 gatctgcacg aacccaagat tgatagtgaa gccatccgaa agcgtcagca gcagctgcga
    26161 ggaaccaaaa tagataaaca gcaccaccag aatttctggc aggccacgca gaatggttac
    26221 cagcgctgaa cctgcccacg cgacaggacg ccattttgcc gactcccata ccgcaaagaa
    26281 catcgccagc gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc
    26341 ggcgcttgct aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta
    26401 gatggtttcg taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg
    26461 cagttcagtg ttgccctgac gtaccgcgat gccgaggcca gtgccgaagt aatctttatc
    26521 ggtcactttg tcgcccaccg ccgccagttt cgggttatct ttcagccact cagtgaccac
    26581 tgcggtgtca ccgaagacgc cgtcgatacg cccgttttgc agatccagtt ttgcgttctg
    26641 gtagctgtca tacggaacgg tagtgatttc cgggtgctta tccataatga atttctggtg
    26701 tgtcgtcccg ttctgtacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt
    26761 gccttgctga cccacaaaca gggctgagtt gtcatagtac ggggtggtaa acagcacctg
    26821 cttttcacgt tctggggtga tatccatccc ggccatcacc gcttccacgc gacggaattt
    26881 caggcttggg atcaggctgt caaatgcctg gttagagaaa gtacaggtcg catcaatctc
    26941 tttacacagc gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat
    27001 cgattcaaac ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc
    27061 ggaaagacta aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc
    27121 gaatcttagt gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc
    27181 gcgtcgcctt gttctacgat atgaccgttt tccatataca ccactcggct ggcggtttta
    27241 cgcgccactt caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca
    27301 cgaatgatgc tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatca
    27361 aacagcagta cctgcggttc catcattaac gcacgggcaa tagcaacacg ctgctgctga
    27421 ccaccagaaa gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc
    27481 agtttttctg cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct
    27541 tcaatcaggt tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg
    27601 ccaacgttac gacgcaaatc gcgaatcgct ttgtctgagg gtgttttggt gaaatcgaaa
    27661 tggttgcctg caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc
    27721 agcagcgagc ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg
    27781 caatccagcg tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt
    27841 aattgaatac tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata
    27901 gtacctttga cagaataatt atgcaatatt tctgctttaa aagttaaaag taaagcgcat
    27961 tattcaataa acatagcaca aaataacggg ggcggtggtc ggcgagcata aatgtcggca
    28021 ttcctcacga aatgccggac aatttacggg gtttattggt tgatcaaggc gttagcgatt
    28081 ctcgatggac tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac
    28141 atcgtcgatc acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg
    28201 gtcaccctgc ttgagatcca cgcgcaatgg aatgttacgg gcatcacgat tcgggatagt
    28261 cgatgcactg gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct
    28321 gcttaacaat tcacgatgac tattatcgcg gctggcatcg ctaagtaggg ttgccagttt
    28381 gtcactcaaa taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc
    28441 atagaattgt tgggccacgc tgtcaggccc cccctggaca caggaaccgg tgcggggacc
    28501 gttatcttta taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat
    28561 aagtagagat agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca
    28621 cactacgacc aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca
    28681 atctgaacac ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag
    28741 ctgacttact taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg
    28801 ttatatggct gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat
    28861 cctcctgtag aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata
    28921 aatgaacgcg gttattgtct aatcattaca aatttttcac atgcgataag agatcctgct
    28981 attaagatga ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc
    29041 agctttgact tttgataata ttatttatgc tacagttgtg ggtataaaat ataatctttt
    29101 tgaacaagtg aatttgagcg aagaataaat caagttgttt cagtgtttcg ttaattaata
    29161 tgagtataaa aggtgttgtg cttttacaat aataaagcgc gaggagttcg gtggattatc
    29221 gtttttcttg ggtgattttt aatatgataa acaatatatc tgatcaagcg tcatcatttc
    29281 catgcacaca attgaatcaa agtgataatt tcctcgattc actgagggaa tttttcgcga
    29341 tattaaattc cagtcgtaag ggtgagttgt ctacatggga taccatctac ttgcatctga
    29401 tattagcaat aaatgctgac agtgatctca taaaaaatga tgtgttgctc gcggaaaata
    29461 ttccatcagc gaattatcaa tttaatacat tttttagcaa tgctttcgag actgatgtta
    29521 aaaaatatct gggtaaatca gaagataacg aagttgaaat aaaagcgggt aatgaaataa
    29581 tctctattgg aatacgtaat gtatcgaatg gaaaacttga aagacaacag tttcttttcc
    29641 cactggacta tgagaataaa ttacaacatc aattagacaa gcattttacg attgaatcgc
    29701 acccattatt atacaggcat actatgggta gtaagattgc gaatgtgatt tttgaaaaat
    29761 tatacagtc
//
LOCUS       DAZFFG010000051        29674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFFG010000051 DAZFFG010000000
VERSION     DAZFFG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.051"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(48..419)
                     /locus_tag="ACHJ7J_002550"
     CDS             complement(48..419)
                     /locus_tag="ACHJ7J_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5000771.1"
                     /translation="MARDIQMVLERWGAWVANNHEDVTWSSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQDLHDLLVDYYVGRMTFMTLARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALSVRLDMDMR"
     gene            complement(409..780)
                     /locus_tag="ACHJ7J_002551"
     CDS             complement(409..780)
                     /locus_tag="ACHJ7J_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5000772.1"
                     /translation="MRIEFVFPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     QLKLNLSGRLAIKIIAEPPDKRRRDLDNIMKAPLDALTHAGLLIDDEQFDEIKIVLGQ
                     LVPGGRLDVKIYEIRGNNYGA"
     gene            complement(793..1842)
                     /locus_tag="ACHJ7J_002552"
     CDS             complement(793..1842)
                     /locus_tag="ACHJ7J_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5000773.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNSRVLVEPEPKSMRG
                     LPSGVVSAVRQPLAEDKSLLPFFSDDRVIRAAGGAGALSDWLLRHVKSCQCPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRAEKIRSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPHIHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWGNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(1844..2122)
                     /locus_tag="ACHJ7J_002553"
     CDS             complement(1844..2122)
                     /locus_tag="ACHJ7J_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000774.1"
                     /translation="MAHDTKLYNSDNTAVFASRHGRRSHTFKYDWYQHDPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(2555..2998)
                     /locus_tag="ACHJ7J_002554"
     CDS             complement(2555..2998)
                     /locus_tag="ACHJ7J_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFO5000775.1"
                     /translation="MQEEEIAYRAINFCNAIGLTASKRKRKRYDMFFESLSIYGVTLD
                     VRTDKEWEALTYDLTIGHCDPASLTITVPNKIYVNACLGEEHALAVIFHELGHLLLGH
                     KAVLHFSAKEPTRIEDAEWQADTFADIVLETIGVRTQQMSFDFYM"
     gene            complement(3034..3393)
                     /locus_tag="ACHJ7J_002555"
     CDS             complement(3034..3393)
                     /locus_tag="ACHJ7J_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5000776.1"
                     /translation="MALTEFGKAVRKARIDTDSTLLTMSQELGTSPAFLSGLETGSKK
                     IPLKWVKKIDSYFKAKGVEIEGLQELAAVANDSVPVNGLSQQQKMLVAGFAKSQFTPE
                     QLKSFADLLQKINNNEG"
     gene            complement(3638..3793)
                     /gene="hokD"
                     /locus_tag="ACHJ7J_002556"
     CDS             complement(3638..3793)
                     /gene="hokD"
                     /locus_tag="ACHJ7J_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5000777.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(4257..4823)
                     /locus_tag="ACHJ7J_002557"
     CDS             complement(4257..4823)
                     /locus_tag="ACHJ7J_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000778.1"
                     /translation="MNTLTKEWLQNTITSIESARDEIPFGLDEDQNNMLTALKIALAS
                     LGAEPVAYIFKHPAWKLFWALTDESNKDQSDVIPVYAAPPAPVVPEGLVKAVRFYEQV
                     KCENPPVETGAWKDAIDWVLKEACCAAILGKADNPPASGNQVSELTMWVKRLASQLEK
                     AKPDCKLPEKAMDYLKRNELINAEDVLR"
     gene            complement(4826..5017)
                     /locus_tag="ACHJ7J_002558"
     CDS             complement(4826..5017)
                     /locus_tag="ACHJ7J_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000779.1"
                     /translation="MNGQISIVRPGACDDCEIRMIIRLERGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(5019..5792)
                     /locus_tag="ACHJ7J_002559"
     CDS             complement(5019..5792)
                     /locus_tag="ACHJ7J_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5000780.1"
                     /translation="MSGIDYQALRDAAEKATTGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVVLALLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRNAKLQSENAY
                     IRNRFKELDLLMGKNILVMQAAIIEWQATGDAKSGLAWIYNTLFGPGELPDESEKDAQ
                     AYFNRKYAPIDEKLMALHKWFWEQSEAERAAANIGVKGK"
     gene            complement(5789..6211)
                     /locus_tag="ACHJ7J_002560"
     CDS             complement(5789..6211)
                     /locus_tag="ACHJ7J_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5000781.1"
                     /translation="MAKVFTQEEREKIKGLVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYNRRQNIICLECRNSEVMQRVLAFYQGNFQEVAQ"
     gene            complement(6252..7322)
                     /locus_tag="ACHJ7J_002561"
     CDS             complement(6252..7322)
                     /locus_tag="ACHJ7J_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO5000782.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQWRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDKEDQN
                     TMVHGVKNATNQSGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTARPEQSHQL
                     QQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVSPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDTEIPPGFRG"
     gene            complement(7394..7819)
                     /locus_tag="ACHJ7J_002562"
     CDS             complement(7394..7819)
                     /locus_tag="ACHJ7J_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5000783.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAEITRAYFELGLTFPELYD
                     DSYPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMKRGGPAGNAVAVH"
     gene            complement(7816..8118)
                     /locus_tag="ACHJ7J_002563"
     CDS             complement(7816..8118)
                     /locus_tag="ACHJ7J_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5000784.1"
                     /translation="MTLKEFIKSLMVGDAKKFAARLGVSPSYLSQMASGRAAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            8216..8587
                     /locus_tag="ACHJ7J_002564"
     CDS             8216..8587
                     /locus_tag="ACHJ7J_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000785.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPAVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            8608..8799
                     /locus_tag="ACHJ7J_002565"
     CDS             8608..8799
                     /locus_tag="ACHJ7J_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO5000786.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            8801..9079
                     /locus_tag="ACHJ7J_002566"
     CDS             8801..9079
                     /locus_tag="ACHJ7J_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5000787.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            9371..9526
                     /gene="ydfA"
                     /locus_tag="ACHJ7J_002567"
     CDS             9371..9526
                     /gene="ydfA"
                     /locus_tag="ACHJ7J_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5000788.1"
                     /translation="MDTIDLGNNESLVHGVFSNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            9528..9656
                     /gene="ydfB"
                     /locus_tag="ACHJ7J_002568"
     CDS             9528..9656
                     /gene="ydfB"
                     /locus_tag="ACHJ7J_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO5000789.1"
                     /translation="MDFDTIMKKAYEEYFEGLAEGDEALSFSEFKQALSSSAKSNG"
     gene            9686..9904
                     /gene="ydfC"
                     /locus_tag="ACHJ7J_002569"
     CDS             9686..9904
                     /gene="ydfC"
                     /locus_tag="ACHJ7J_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5000790.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            9927..10256
                     /locus_tag="ACHJ7J_002570"
     CDS             9927..10256
                     /locus_tag="ACHJ7J_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000791.1"
                     /translation="MEFKDLPMQFQEMAANIVRSQLATLDLNTVEKETIDTISGNVRR
                     AFIGLYEEKHLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENYFATKSINS
                     FDSKQEN"
     gene            complement(10225..10662)
                     /locus_tag="ACHJ7J_002571"
     CDS             complement(10225..10662)
                     /locus_tag="ACHJ7J_002571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000792.1"
                     /translation="MNQLPRRALSRYGLTFQGNILSVNCQCRMLTRVRRTHSTSPVEN
                     SLITNPQEGEMSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVMDKNE
                     VVRQSLVDYIDSLQPGTFNHESFNHEKEHVKSVINSLVLNQKN"
     gene            11067..11288
                     /gene="kil"
                     /locus_tag="ACHJ7J_002572"
     CDS             11067..11288
                     /gene="kil"
                     /locus_tag="ACHJ7J_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5000793.1"
                     /translation="MIAHHFGTDDIPRQCVTPGDYVIHKGRTYIASVNNIKKQRLYIR
                     DFSTQHCIKETMIKVFLGRDGLPVKAESW"
     gene            11412..13883
                     /locus_tag="ACHJ7J_002573"
     CDS             11412..13883
                     /locus_tag="ACHJ7J_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5000794.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDNLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDIATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKINPVIAATHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTEYKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYSGSQQNTGTSDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDADELLAVSRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQEMEQNDQKAEQNSQNALQKEPETKQSEPVAQQEPEKVCTACSQSGGGNCPDCG
                     AVMGDATYQETFDEENQVEVQENDPEEMEGAEHPHKENPGGNQHHASDNKTGEATDPL
                     IKVNGHHKLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIAENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYCNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPSQADF"
     gene            13942..14145
                     /locus_tag="ACHJ7J_002574"
     CDS             13942..14145
                     /locus_tag="ACHJ7J_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO5000795.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            14145..15195
                     /locus_tag="ACHJ7J_002575"
                     /pseudo
     CDS             14145..15195
                     /locus_tag="ACHJ7J_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(15223..15312)
                     /locus_tag="ACHJ7J_002576"
     tRNA            complement(15223..15312)
                     /locus_tag="ACHJ7J_002576"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15276..15278),aa:Ser,seq:cga)
     gene            15406..16203
                     /gene="mtfA"
                     /locus_tag="ACHJ7J_002577"
     CDS             15406..16203
                     /gene="mtfA"
                     /locus_tag="ACHJ7J_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO5000796.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            16304..16379
                     /locus_tag="ACHJ7J_002578"
     tRNA            16304..16379
                     /locus_tag="ACHJ7J_002578"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16337..16339,aa:Asn,seq:gtt)
     gene            16690..24690
                     /locus_tag="ACHJ7J_002579"
     CDS             16690..24690
                     /locus_tag="ACHJ7J_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5000797.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLVTQLVFPMAAA
                     AQGVVNAAIQQPVPAQIAIANTNTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTLTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFVVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLPVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKTVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFNLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSRTNSAEMTNGGQAVTN
                     EQGKATITYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLHALF
                     DTVSAGETTSLYIEVKDNYGNGVPQHQVTLSISPSEGVTLSNNGIYTTNYYGYFYASF
                     TATKAGVYQVTATLDNGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAVTDTEGKAKVMLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEVV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKMAGFTASSSSFTASTTEGATLTASVTDAYGNPLEGIKVNFRGPATTLSN
                     TSVETDAQGKAEILVTSTIAGTKVVTANLAIAPTEAAIRMLTVNADVDSATITSLEMP
                     EGQVIIREPIAVKAHVDDQFGNPVADQLVTFSAEPSSFNMVISQDTVSTNRQGIAEVT
                     MTPGRYGSYTVKASLANGSFYEKDLVVIDLRLTLTSSSPLIGVNDPSGATLTVRLTHA
                     NGAPLSHELVTFSVTPEGATLSSQTATTNTSGEAQVVLTSNKVGTYVVTASIHSGVII
                     QTQTTVKVTGNPSTAHVASFIADPSTLTANNSDISTLKATVEDSSGNLVEGVNVNFVL
                     KSGSATLTSLTAVTDQNGLATTSVRGAMTGSVTVSAETSYGGAQTVDITLVAGPADAS
                     LSVLKNNRSSLKGDFTESAELHLVLHDLSGHPINVSEGMEFVQSGTNVPYVQVSAIDY
                     SKNFSGEYKATVTGGGEGIATLIPVLNGVHQAGLNTTIEFISAETRPMTGTVSVNGAN
                     LPTASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSSTASWVGVDATGKVTFKNDGDSN
                     TVEITATPRSGGAIYQTQVRVKGWWKDNNNIILPLSRAENYCNNEIGNGYAIPGVNLL
                     SSGENRREIGSLFGEWGDMGHYMDADFYSEIYWSSNTAGGGRQYIVSLENGAHGSVQT
                     SEYFHVACYKKS"
     gene            complement(25272..26324)
                     /locus_tag="ACHJ7J_002580"
     CDS             complement(25272..26324)
                     /locus_tag="ACHJ7J_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO5000798.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            26639..27955
                     /gene="shiA"
                     /locus_tag="ACHJ7J_002581"
     CDS             26639..27955
                     /gene="shiA"
                     /locus_tag="ACHJ7J_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO5000799.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPENKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            28057..29511
                     /gene="amn"
                     /locus_tag="ACHJ7J_002582"
     CDS             28057..29511
                     /gene="amn"
                     /locus_tag="ACHJ7J_002582"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO5000800.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         8001 a         7208 c         6898 g         7567 t
ORIGIN      
        1 cacattaatt ataaaagcag aaaacataac catcgcaata tgtattccta ccgcatatcc
       61 atatctaacc tgacactcag agccataagc atcccttcaa taataccttc cgctttataa
      121 agtcttttac caatcagtcc atcagaacat ctgtgcttac gtgcaagtgt catgaacgtc
      181 attctaccta cgtaataatc taccaacaaa tcgtgcaaat cctgattatt cttgtttaat
      241 cgtgccatac agccacaaat tatcatggca tcatcatcag aacactgagg acgtgatttc
      301 actttagtcg ggatcaatcc tttaaaaccg gcagcaatag atgaccatgt aacatcctcg
      361 tggttatttg ccacccaagc tccccatcgt tccagaacca tctgaatatc acgcgccata
      421 gttattacct ctgatttcgt aaatcttcac atccagcctc ccaccaggaa cgagctgacc
      481 gagcacaatc ttgatttcat caaactgctc gtcgtctatg agtagtccgg catgcgtcag
      541 cgcatccagt ggtgctttca tgatattgtc caggtcacga cggcgcttat ccggtggctc
      601 tgcaataatc tttatcgcca gccttccgga caggtttaat ttcagttgct gctggcgaac
      661 aataagtgcc acatcacggc gataacgctc accggctttt gatacaaaat atgtgctgcc
      721 acgacgtcgc cagtaggtgt tcaccgtcgg cgggtaagga aaaacaaact ctatacgcat
      781 cattaacctc ttttacccaa gcacgccggt tgcaaaggcg tgatcaagaa aacgaaaaat
      841 taaatcaacc tgagaaccat gcttttcttc gaacgccagc ggatccgcat gaagctcgtt
      901 atgatgttcc cgacacagcg gtaacgtaaa aatatcgtgg gcttttgttc ccattccgcc
      961 ctgaccgtga ccaataaggt gatggggatc gtcagctggc ttaccacagc acgcacacgg
     1021 ctgagtcttt acccagcgtg tgtatttctc attcccccaa cggcgacgtt taggtcgctt
     1081 cataaaagat tccggagact caggatcaac ggcaatgctg accaccgtct tttcctgtgg
     1141 cgggttctgt tgctggtgga tgtgaggcaa cggcgcaaga ttttttgtgc gctgcttcag
     1201 tatgctggtg gctgtctgct ctcccggtac gatgtcgctc tcgcggtaca cagagcggat
     1261 tttttccgca cgtaacccca gagaacgacg taatactgcc tccggtagtg cgtccgctac
     1321 ctgattgcag accgcccacc aggataattc agccagcgat aattcccgtt cctgtgtacc
     1381 gcttattgcg tgacgtatga cgtcaatcat ccaggctgtc agattttgtt gagcaagctg
     1441 cccgagtgat tcggaggtct ggtcacgcag ctggttgtca caatgccagc acaacaccat
     1501 cgcaccggtg ccgtaacgat gtatgacggt ttcactgtga tggtagtcac catgagggca
     1561 ctggcaggat ttaacatgac gcaggagcca gtcagacagt gcaccagcgc cgccggcagc
     1621 acgaatcacc cgatcatcgc tgaaaaatgg cagtaatgat ttgtcttccg ccagcggctg
     1681 gcgaacggca gaaacgactc cggacggtaa accacgcatg ctttttggtt ccggctccac
     1741 cagcactcga gagttatgaa atacctgcat ggattcacgg cccggcttaa gaaccaccag
     1801 cccaagttcc ggtaccggaa caggtcgaag caatacccgc acgttacctc cagatgcgtt
     1861 gctggaatgt gcgggacgga cgcggtgggc gttcggaata agggagtctg acgtagatta
     1921 tccagtgacg ataatcaagg ctgagggctt tcctaaactc atacccacgt ctgcggtagt
     1981 tctgaatcag ccattcggcc tgttcttcag tgcaggggtc atgctgatac cagtcatatt
     2041 tgaatgtgtg agaacgccgc ccgtgcctgc tggcaaagac ggctgtatta tcagaattgt
     2101 ataatttggt atcgtgcgcc atcggttgtc tctgctggcg cagcaggtgc cagttgttca
     2161 ggctggcatg tggattgtaa accaagatgc tcagaaaaaa caaaacccgc taaagcgggt
     2221 tttgtcagta ctgaatatca agctgcgtgc ttgccactac aactgcaaag tgggatcggg
     2281 aaaggcttcc cttttttcgc gtggcgtatt acaccatcta cacacacaga gtagcgaaaa
     2341 atgatctcac atggacagcc acatttgcga caggtaccca ttgccatgat ttcgttttcc
     2401 ctgctcacct gtgaatccac acaggttgca ctttgttggg agaaccacta cacttcgctt
     2461 acctacaaaa aatgttgtga ttggattatc tccatacact acggaatgtg ttagcgcact
     2521 ttccgcccct aaaagccctg ttagcgcagg gcttttacat ataaaaatcg aacgacatct
     2581 gttgtgttct cactccaatt gtttccagta cgatatccgc aaatgtatct gcctgccatt
     2641 ctgcatcctc aatacgagta ggttctttag ctgaaaagtg taaaactgct ttgtgtccaa
     2701 gaagcaaatg acctagctcg tggaagataa cagcaagtgc atgctcttcc ccaaggcaag
     2761 cattcacata tattttattg ggaaccgtaa ttgttagaga agcggggtca caatgtccga
     2821 tagttagatc atacgtcaga gcctcccact ccttgtcggt cctgacgtca agagtaaccc
     2881 cataaattga aaggctttca aagaacatat cataacgctt tcttttgcgc tttgaggctg
     2941 taagaccaat agcattacag aagtttatag ctctgtaggc tatttcttct tcttgcatcg
     3001 gtgacaccct gttacctctc atttgataca taattacccc tcattgttat taatcttttg
     3061 caataaatct gcaaaactct ttagctgttc cggagtaaac tgcgacttag cgaatcccgc
     3121 tacaagcatt ttctgttgct gagacaaacc gtttactgga actgagtcat tcgcaactgc
     3181 cgctaactcc tgtaaccctt caatttctac acctttggcc ttgaagtaag aatcaatctt
     3241 cttaacccac ttcagaggta tttttttgct accagtttct aaaccgctaa gaaacgctgg
     3301 cgaagtgccc agttcttgag acatagtcag aagagtactg tcagtatcaa tcctcgcttt
     3361 tctaacggcc ttaccgaatt cagtgagtgc catgatttta tcctcaattt gttgatttag
     3421 cagacttgcc taacgcaagc ctgatgggcg cttaaattct aatttctaaa atttaccata
     3481 cacaaaaaca aagtaaacct ttttagttaa aaaaaatctc ttttaaggga attttttcac
     3541 aaaaagaaca aaacccgccg aagcgggtta agtgtgggtg cattgaggat gcctgacaca
     3601 tcagaggtgg cgagggattt ctcccccgcc tggtctctta ctcctcaggt tcgtaagctg
     3661 tgaagacagc gacctccgtc tggccggttc ggattcgtac ctcgcagagg tctttcctcg
     3721 ttaccagtgc cgtcactatg acggttaaac agatgacgat cagggcgatt agcatcgcct
     3781 tttgctgctt catagcctgc ttctccttgc ctttcggcac gtaagaggct aacctacatg
     3841 tgttcagcat ggattgagcc tcagattaat gttaagcgtc ttgccggacg cgtaatgtta
     3901 actggggtaa ctggggcttt tctctgtctg ccttacagtg gcatgcccga ggcagacagc
     3961 ctcaagcacc cgcaacaatc ttaccgacac cgataagaaa acgctatttt ttattgccag
     4021 aaccttcttc ccgggctaat gtatccgcgt cagaacggcg caatgcgctc gcctgagata
     4081 cgtttacttg tcattggtgg caacagataa cggcaatcgt tgtttctgtt tgtttccttc
     4141 aaaaaccccg gaccgtcaat ccggggtttt tatttgttat ccccagcggc aaatcgaata
     4201 caccaccagc gccaccgcca tcgcaattcc tacctttgtg aatgcttcag gctaggtcat
     4261 cgcaaaacat cctccgcgtt tatcagttcg ttccgcttca ggtagtccat cgccttctcc
     4321 ggtaatttgc agtccggctt agctttttcc agttggctgg ccagtcgttt aacccacatt
     4381 gttaattcgc taacctgatt gccggatgct ggtggattgt cggctttacc caaaatggca
     4441 gcacagcagg cctctttgag cacccagtca atggcgtctt tccatgctcc ggtttcaact
     4501 ggcggattct cacactttac ctgttcataa aagcgtaccg ctttaacaag cccttcaggc
     4561 actaccggcg ctggcggggc ggcataaaca ggaataacgt ccgattgatc tttattgctt
     4621 tcatccgtta aagcccagaa taatttccag gccggatgtt tgaaaatata agcaactggt
     4681 tctgctccca gtgatgccag tgcaattttt aatgcggtaa gcatgttgtt ttgatcttca
     4741 tcgagtccga acggtatttc atcccgtgct gactcaatgc tggtaatcgt gttctgtaac
     4801 cattctttgg taagagtatt cataactatt tcactttaat ctcaatattt cgcagcttta
     4861 gctctactgg aaggtctgac tttcctgtta atgctaatgc gagattttca ggagtaatga
     4921 gagcagttat tgttttcccc ctctccagac gaataatcat tcgtatctcg caatcgtcac
     4981 atgctcccgg tcgaacaatt gagatttgtc cgttcatctc acttcccctt cacaccaatg
     5041 ttggcggcgg cgcgctcggc ttcactttgt tcccaaaacc acttgtgaag cgccataagc
     5101 ttttcgtcaa tcggtgcata tttgcgatta aagtaggcct gagcatcttt ctcagattcg
     5161 tctggtaatt cgccagggcc aaacagtgtg ttataaatcc atgctagtcc gctcttagcg
     5221 tcgccagttg cctgccattc gataatggca gcctgcatga ccagaatgtt tttccccatt
     5281 aataggtcca gttctttgaa ccggttgcgg atgtatgcat tctcgctttg taattttgcg
     5341 ttgcgctttt ctgaggcttc aagtaacgcc tgcttatcgc gtagcgcttc ttccagttca
     5401 gcaacatggc attcactatc aataaggttg ttctctgctg cttcaagctc aacacgcagc
     5461 ttccctaccg ttagcgcaat ttcctcattc tcctggtcgc ggcgtttgat gtattgctgg
     5521 tttctttccc gttcatccag cagcgccagc acaacatttg gattaaaggc agcaataaat
     5581 tcagcgtttg cataagcctg agcatctgtt tcaaccaggc agttaacatg acattctgca
     5641 attacgccac cgggttctcc tttccatttt tgacaaacaa aaactcctgt taaattaccg
     5701 tgctggttaa cagatgtatg ccctacgatg tagctccctg tagttgcctt ttctgccgca
     5761 tcacgcagtg cctgatagtc aatcccgctc actgtgccac ctcctgaaag ttaccctgat
     5821 aaaacgccag tacacgctgc ataacttcac tattccggca ctcgagacag attatgttct
     5881 gtcgtctgtt gtaacgacgt atttctccat caggtaatga ccagataagg tccggatcaa
     5941 ccgcagatgg tttcttcagc tttgctcttg agagcttttt acgggcattt tgccagtcct
     6001 tacgcgcctg ttcagacggg aataacccgt agccagaatt gtatacatcg ccactggcaa
     6061 ccagctctct ggcgagaaca ctcatcagat atcttgtcgc acctgttttc gcttccagtt
     6121 gtcgtaacgt ctcgcgtcca ctctggcgta cgagttcaac aaccagccct ttaatttttt
     6181 cccgctcttc ttgtgtaaaa acttttgcca caagccctcc tgaaaattac ctcatgacca
     6241 gaaattaaca cttaccccct gaagcccggc ggaatttcgg tatccggttc agaaatatga
     6301 ttcacacaac gctggttgtt cgtgccgctt accgggagca accaggggtt ctcaaaattc
     6361 cggtccggtc caaaaaacgt cgtcgctcgc tgaacaaatt ccgttcccgt tttcccggta
     6421 gccgccaggt atcttgcgta acgcctcacg ccatccagca tggcctctgg tgacaccccc
     6481 tcgcgtaatc tggccttcca ggcactgaaa gcggatttct tcgggtttgc cccagcacgc
     6541 aacgggtatt cccgccagac ctgttcgaac acatccggat aatccactcg tcccacaggc
     6601 tgcccggtgt tttccgggac tacccgatcg gcttcccgct gaatggcgga atcggcttca
     6661 ggctgctgca gttggtgtga ttgctccggc cttgcggtca tcacctgctg cacagcgccc
     6721 gaatcggctt tcagcgcata cgctgaatcg gcttccggtg tcgtgcctgc tggctgacca
     6781 ggattgacgg tctgaacatc ccctgactgg ttcgtggcgt tttttacgcc atggaccata
     6841 gtgttttgat cttctttatc tgtatcttta tctgtatctt tatctgtcgt gactcgtcgt
     6901 gacatgtgcg tgacatttcg tgacgcgccg tgacaatcgc cattttgttc ccgctttctt
     6961 tccctctctc gctgcgccct cttgcgctct gcaggagatt ttgcggtttg cgaaatattg
     7021 ccgttgtcct ctttcagcac ctggcgtttt tcccatccag tgattaaatc accatcaagt
     7081 acccgccact gcatcgtctg caaaattgaa tcaattacct cttctgtcac gtcgagcgca
     7141 cttgccaaat cttctgtcgt gacatcaatg tgacctcgcg tgacatttcg tgacgcgctc
     7201 accaagaggt ggatatacac tgccatcact gttgcaattg gctgccctga caccctggca
     7261 attgttcgcc acttagggtc atttggcatg tcatgccata atctgagcca ggcgttagcc
     7321 atactcacct cttctgatac cgaatctttt tactcacgag ttgccggaag cgattcgata
     7381 tggctattgt cagtcaatgt actgccacag catttcctgc cgggccacca cgtttcatct
     7441 gattgaaacc ggcgattgcc actgcgacaa aatcatcagc gtctctcacc agtcgctccc
     7501 gcgtctccac cagttcccga aaataagctg aactgtggct gcgcattctg gccaccagca
     7561 aaggtggcat tgccttttcg atcgctggta acaacgcctg aattttttca acagcatcag
     7621 gggtgtcttt ctccacccag cggaaaattt tctgggtatt acgagccagg gcttccggat
     7681 agctgtcgtc gtacagttca ggaaacgtca gccccaactc aaaataagcc cgggttattt
     7741 cagctgccgg aactttttca ccgtccggat gcgcccagac attcatcgcc atgcggatgt
     7801 gttcatgctt gattttcatg aatcaactcc atcagataag catgcactac aatcaccttc
     7861 agcatgaact acatgtgttt gccccaaacg aatgccgctc gcatactcag gccaaataag
     7921 ctcccaatca tggggtcgta gctccgccct acttacttgg ccttccgtcg cagattcgat
     7981 cataagggcg cgggttggag atatagctgc tcgtccagac gccatttgcg ataagtaaga
     8041 tggcgataca ccaagtctgg ccgcgaattt cttagcatca ccaaccatca atgatttaat
     8101 aaactctttt aatgtcatac cttcctcggt ttagtgtttt tttgcgagtt tagtgtttaa
     8161 taaaccatta agtcaagtat ttgcttgttt agtgattact aaagataatt accacatgca
     8221 aaaaaaagaa attcgccgtt tacgtctcaa ggagtggttt aaagataaaa ctctgccacc
     8281 caaagagaag agctacctat ctcaactaat gagtgggagg gcctcgtttg gagaaaaggc
     8341 tgccagaaga atagagcaaa catacgggat gccggaaggg tatctggatg cggaatacgc
     8401 agaacaaccg gcagtttctc caccacatgc agggttaacg tctaatcaac tggaattatt
     8461 gcagattttt tcagccttcc ctgaggatga gcaacgccag ataatcagcg agttaaagca
     8521 gaaaaaagaa tcaatggaag atctaatagc gagatggatt gcggcgcaaa aatgccgccg
     8581 cgcctgagtt ataaaaccgg aggaaacatg aatagagccc tttcaccaat ggtttctgaa
     8641 tttgaaacca ttgaacaaga aaacagttac aacgaatggc tgcgtgcgaa agtagcaacg
     8701 agccttgcag atccgcgccc agcaattccc catgacgaag ttgagcgcag aatggcagaa
     8761 cgctttgcta aaatgcgcaa ggaacggagc aaacagtaaa atgttacccg tgttatggct
     8821 tgaaagcgca gataccgacc tagatgatat aactagttat attgctcgtt tcgacataga
     8881 tgcggctgaa cgcttatggc agcgattaag gggttgtgtg ctgccgttat ccgaacatcc
     8941 gtatttatac ccaccaagcg acagagtacc tggcttgcgt gagattgtag cccaccctaa
     9001 ctatataatt ctataccgcg taacaacatc aagcgttgaa gtagtaaacg tgatccacgc
     9061 aagacgccag tttccctaac tttcactacc aatagaaaca taacaaccgc aacgacttta
     9121 tcaaaagcgt tgtgtttgtt acgctccgtg gtttagtttt tacttgactt aagtttaatg
     9181 tttattaaac taaaaatacc aacccacccc gccccacaga atgcagggca atacttcgag
     9241 ttaccaggca gtggtcaggg gttaagtagc cagcccgagg cgtaagaaca tgacggcagg
     9301 gttcaacttt aataactatg cagcaggttt ttgttccgct accccggcgt taaggggaaa
     9361 ttaggtcaac atggatacta tcgatcttgg caacaacgaa tctctggtgc acggcgtgtt
     9421 ttccaaccag gacggcacat tcaccgcgat gacgtatacc aaaagcaaaa cgtttaaaac
     9481 cgaaaatggt gcccgtcgct ggctggaaag aaactcaggt gagtgatatg gatttcgaca
     9541 caatcatgaa aaaggcttac gaagaatact tcgaaggcct tgccgaaggc gatgaagctc
     9601 tcagcttcag cgaatttaaa caggcgcttt ccagttcggc aaaatctaac ggctgataag
     9661 cgaaacagca ccgcgaggaa tcagtatgca gaaacgagaa cccgtcatca tcgcgccaga
     9721 ttataccgat gatgaacttt atgagtggat gcgccagaaa attaatgcag cgcaggatct
     9781 gaaatgggcc aatgaagcca gggctaagca ggctgaaaat ctgtccgctc tggagcagga
     9841 tatcaccaat ctggaaaaag cagcggcatt aagcattgcc agaatgatta catacccacg
     9901 ctaatagcta accaacgagg ctaataatgg aatttaaaga tttaccaatg caattccagg
     9961 aaatggcagc gaatatagtt cgttcccaac tggcgactct tgacctgaat accgtagaaa
    10021 aagaaaccat cgatactata tccggtaacg tgcgtcgtgc ctttatcggt ctgtacgaag
    10081 agaagcacct ctctgataac caggatttac atgaaaaata cttcctggat ctaatggaca
    10141 tcattgataa ggggtttggc ttgttaatga aaaagaaagg gattcgaata gaaccccttg
    10201 aaaattactt tgcaacaaaa agcattaatt cttttgattc aaaacaagag aattaattac
    10261 agatttaaca tgctctttct catgattgaa gctctcatga ttgaaagtgc cgggttgaag
    10321 cgagtcgata taatcaacaa gactctgtcg tacgacttca tttttatcca taacagatgc
    10381 aagaaatgaa attgctaaaa gagttatatc actacgcgcc gcagcatgct gcaatgcttt
    10441 atcaaaatta ttaatctggc gtatcaggga gttaatgatt tcatcatttt cagtcgacat
    10501 ttcaccctcc tgagggttgg tgattaagga gttctccacg ggtgaggtgg agtgcgtgcg
    10561 ccggacacgg gtgagcatcc ggcactgaca gtttactgaa aggatatttc cctgaaaagt
    10621 cagaccataa cgcgaaagcg cacggcgagg tagttggttc atagatagcc tgtcgttaaa
    10681 ttttcgtcga ccgtgcgctt ccggttgtgg cactccgcga aatggcgcgg cggtaagtat
    10741 ggcggtgtta ttccttcccc gttgaggaca ccgggttgtc aggttgacca tacgcttaag
    10801 tgacaactcc gctgcaacgc cctctgttag caattttctg gtgacgtttg gcggtatcag
    10861 ttttactccg tggctgctct gccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc
    10921 ggctgagcgc acgcggaaca gttaaaacaa aaaacagtgt tatggggtga ttctctgtat
    10981 ccggcgttaa ttgttaactg gttaacgtca cctggaggca ccaggcaccg catcaacaaa
    11041 gttcatttgt aaaaatggag ataattatga ttgctcatca cttcggaact gatgatattc
    11101 ctcgtcagtg cgtaactccc ggtgattatg ttattcataa aggaagaaca tatattgctt
    11161 cggtaaacaa cattaaaaaa caacggctct atattcgtga tttttccaca caacactgta
    11221 ttaaggaaac catgattaaa gtcttccttg gtcgtgatgg tttacctgta aaagcagaat
    11281 catggtgagc agtaataaaa taactgccac aatacgacat tcagcttaat gaatacatca
    11341 gatttgattc ttatatgcca gcaatggcag ggatttgttc atccttaaat ctgtcatgag
    11401 gttaaaacaa aatgagtaaa gtctttattt gcgctgccat tccggacgaa caggcaataa
    11461 aggaagaagg cgcagtcgct gtagccactg ccattgaagc cggtgatgaa cgtcgcgccc
    11521 gcgcaaaatt tcactggcaa ttcctggaac attatccggc tgctcaggac tgcgcttata
    11581 aatttcttgt ctgcgaggat aaacccggta taccccgccc tgccctcgat tcctgggatg
    11641 ctgaatatat gcaggaaaac cgctgggatg aggcgtctgc ttcctttgtc ccggtcgaga
    11701 ctgaatccga tccgatgaac gtcacttttg acaacctggc ccctgaagta cagaacgctg
    11761 tcatggttaa gttcgacaca tgtgaaaaca tcactgttga tatggtgatt agcgcacagg
    11821 aattgttgca ggaagacatc gcaacattcg acggccatat cgttgaagcg ttgatgaaaa
    11881 tgccagaagt taacgccatg tatcctgagc ttaagctgca tgccatcggg tgggttaagc
    11941 ataaatgtaa gcctggtgcc aaatggcccg aaattcaggc agagatgcgc atctggaaaa
    12001 aacgtcgcga aggtgaacgc aaggaaaccg ggaaatacac gtctgttgtt gatctcgccc
    12061 gcgccagagc caatcaacag cacactgaaa attcaacagg aaaaatcaac ccggtcattg
    12121 ctgccactca tcgcgaatac aagcagacat ggaaaacact ggatgacgaa ctggcctacg
    12181 ctctctggcc tggtgatgtg gatgccggaa acattgacgg cagcatccat cgctgggcaa
    12241 aaaatgaagt tatcgacaac gaccgagaag actggaagcg tatctcggca tcaatgcgca
    12301 aacagcctga tgcccttcgc tacgaccgcc agactatttt tggccttgtc cgtgaacgtc
    12361 cgatcgacat tcacaaagac cctgtggcac tgaacaaata cattactgaa tacctgacta
    12421 caaagggcgt gtttgaagat gaaggaagaa atcagagcgc aactgatact ctctcgtcgc
    12481 cagtaccaga aactgatgca gtggaaacgg caattccgga caacgaaaaa accgaataca
    12541 aagtggaagt cgaaccatct gtagagcgtg aggggccttt ctacttcctc ttcaccgaca
    12601 aggatggcga aaaatacggt cgcgcaaaca aactttctgg tctggataag gcgctggctg
    12661 ccggggctac tgaaatcacg aaagaagaat atttcgcccg caaaaacggt acatactcag
    12721 gctcacaaca aaatactggt acatctgaca cgaccgcaca accagagccg gtaaaagtta
    12781 ccgctgacga agtaaacaaa attatgcagg cagccaatat cagccagcct gacgccgatg
    12841 aactgcttgc agtatcacgt ggtgaatttg ttgcagggat tagcgatccg aatgatccga
    12901 aatgggtgaa ggggattgaa acccgcgatt ctgtgaacca gaaccagcaa gaaatggaac
    12961 agaacgacca gaaagcggaa caaaacagcc aaaatgcgtt acaaaaagag ccagaaacga
    13021 aacaatccga accagtagcg caacaggaac cggaaaaagt ctgcaccgcc tgcagtcaga
    13081 gcggtggcgg caactgccct gattgtggcg cggtgatggg cgacgcaaca taccaagaaa
    13141 cattcgatga agagaatcag gttgaagttc aggaaaatga tccggaggaa atggaaggcg
    13201 ctgaacatcc acacaaggag aaccctggcg gcaatcagca tcacgccagc gataataaaa
    13261 ctggcgaggc gacagatccc ttaattaagg tgaatggtca tcataagctc acatccacca
    13321 gcagagcggg gattcatctg atgatcgacc ttgaaaccat gggaaaaaat cccgatgccc
    13381 cgattatctc aataggcgca atatttttcg atccgcaaac cggagatatg ggaccggaat
    13441 ttagtaagac tatcgatctg gaaactgccg gcggagtcat tgatcgtgac accattaaat
    13501 ggtggcttaa gcaatcacgc gaggcgcaat ctgccattat gaccgatgaa atcccgttag
    13561 atgatgcact actgcaattg cgggaattta tcgccgaaaa ctccggtgag ttttttgttc
    13621 aggtctgggg aaatggagcc aacttcgaca acacgatttt gcgccgttca tacgaacggc
    13681 aggggatccc ctgcccgtgg cgctactgca acgatcgcga tgtacgcaca atcgttgagc
    13741 tggggaaagc catagacttc gatgccagaa ctgctatccc attcgaaggt gagcgccata
    13801 atgcacttga tgacgctcgt taccaggcaa aatacgtttc agctatctgg caaaaactga
    13861 tcccgagtca ggctgatttt taatgttcaa ccctaattgc cgctaaccgt atatagttag
    13921 cggcggttat gagatatagc tatgagcagc ttatttttaa ccgaagatga attgctaata
    13981 ttaacgggct gcaaatatgc aagccaccag cgaaaatggt taacggaaaa cgggcttccg
    14041 ttctatacca atcgtagtgg caaaccgatt gtcagccggg atctatttac ctgcaataaa
    14101 actttaccac cacgcgaggt agagccgaat tttggtgcga tctgatggga agacgaagga
    14161 aaaatcctga acacgaaaaa ttacctccaa atgtataccc aaataaatat agttatgtat
    14221 ggaaaccaac atccagagaa tctgtcacac taaccgccat caaggatggt ttagctgctt
    14281 tatggaaaaa gtatgaggaa actgtaaata atcgcgatcg tgcaatgaca ttcggtcgct
    14341 tgtgggaaaa attcctcgcc agcgcctatt acagtgacct tagtccaaga acacaaaaag
    14401 attatctgca acatcaaaaa aagttgcttg ccgtattctg taaggtacca gcggattcca
    14461 taaaaccaga acacatccgt cgatacatgg acaaaagagg ggagcagagt aaaacgcaag
    14521 ccaaccatga aaaaagcagt atgtcccgtg tttacagttg ggggtatgag cgagggtacg
    14581 tgaaggctaa cccatgtgca ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg
    14641 taaccgacaa agaataccag gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa
    14701 tggaaatttc ctatctgtgt gcagcgaggg tttccgatgt gttatcgctg aaatgggagc
    14761 agattggaaa cgacgggatc tttatccagc aagggaaaac agggaaaaaa cagataaaag
    14821 catggagtcc acgattacag gcggcgatcg aaaaagcaaa acagttacca acatccgcct
    14881 atgtaatcag caatcaatac ggcaaccgat atatgtacaa aggctttaac gaaatgtggg
    14941 tagaagcaag aaatcgcgca ggcaaaattt caggtatttt aaccgacttc acctttcatg
    15001 atctgaaggc aaaaggaatt tcagactatg aaggaagcag ccgggataag caacttttct
    15061 ctggtcacaa aaccgaaggg caagtgctaa tctatgacag gaaggttaaa gtttcaccaa
    15121 cacttgatgt cccgttacct gaaaatattc caagaaaata ttccaagtaa ttccaagtgt
    15181 gatttttgtc actgacttaa tgatgtgtaa gtgattgaat tttggcggag agagggggat
    15241 ttgaaccccc ggtggagttg cccccactcc ggttttcgag accggtccgt tcagccgctc
    15301 cggcatctct ccgttcagat ggttgccatg atgccaggaa atttggcatt ttaacagtcc
    15361 ctgtccgtgc aattttgttc aagtgacgag tttgcgagca aaacgatgat taagtggccc
    15421 tggaaagtac aagaatcagc acatcaaact gcccttccct ggcaggaagc actatcgatc
    15481 ccccttttaa cgtgtctgac ggaacaggaa caaagcaaat tagtcactct tgccgaacgt
    15541 tttttacagc aaaagcggct tgttccttta cagggctttg aactggattc attaagaagc
    15601 tgccggatag cacttctatt ttgcctaccc gttctggagt taggactgga atggctggat
    15661 ggttttcatg aagtcttaat ttatcctgcg ccatttgtgg tcgatgatga atgggaagac
    15721 gatatcggtc tggtgcataa ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg
    15781 cctatcgttt tgaactggtt ggatatacaa gattcttttg atgcttctgg ttttaacctg
    15841 attattcatg aagtcgctca taagctggac acccgtaacg gcgatcgcgc cagcggagtt
    15901 ccctttattc cgttgcgtga ggttgctggc tgggaacacg atcttcatgc tgcaatgaac
    15961 aacattcagg aagaaatcga attggttggc gagaatgcgg cgagcattga tgcttatgct
    16021 gccagtgatc ctgctgaatg ttttgccgta ctttctgaat atttctttag cgccccagaa
    16081 ctttttgctc ctcgtttccc ttcattgtgg caacgtttct gccaatttta tcaacaagat
    16141 cctttgcaga gactgcatca cgctaatgat acagactcgt tttcggcgac gaatgttcat
    16201 taattaacaa ctttgcagat taattaacca attgaaatga cttatgaaat ttagtgttga
    16261 cagacaaggt accgctaagt aatatgcgcc ctgttcacac gattcctctg tagttcagtc
    16321 ggtagaacgg cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa
    16381 attctaaaaa ttcgcttctt agcgcaatgt cactgacctt aattgagcat tgtttttcaa
    16441 cggatagcgg gttttgacat cttaagcgtc ctcgaccttt atggttgagg gcgttttgct
    16501 atggcgccat caccattttc acctcgatta taaaacttga gtaattcagt agtctcccct
    16561 cttgcaactc acacccaaaa ctgcctaacg aaaagttatt aattttcaat catattgcta
    16621 tcaggattta cattttttcg ctgtgctaga aagggcgcat ttatgttagc tcgttcaggg
    16681 aaggtaagca tggctacgaa gaagagaagt ggagaagaaa taaatgaccg acaaatctta
    16741 tgcgggatgg gtatcaaact acgccgctta actgcgggta tctgcctggt aactcaactt
    16801 gtgttcccta tggctgcggc agcacaaggt gtggtaaacg ccgcaatcca acaacccgtt
    16861 cctgcacaaa tcgccattgc aaataccaat acggtgccct acaccctagg agcgctggaa
    16921 tcggcccaaa gcgttgccga acgtttcggt atttcggtgg ctgagttacg caaactcaac
    16981 caatttcgta cgtttgctcg aggttttgat aatgtccgcc agggtgatga actggatgtc
    17041 ccggcacaag ttagtgaaaa aaaattaacc ccgccgccgg gtaatagcag tgacaacctc
    17101 gagcaacaga tagccagtac ttcacagcaa atcgggtctc tgctcgccga ggatatgaac
    17161 agcgagcaag cggcaaatat ggcgcgtgga tgggcctctt ctcaggcttc aggcgcaatg
    17221 acagactggt taagccgctt cggtaccgca agaatcacgc tgggcgtgga tgaagatttt
    17281 agcctgaaga actcccagtt cgattttctc catccgtggt atgaaacgcc tgataatctc
    17341 tttttcagtc agcatactct ccatcgtact gacgagcgta cgcagattaa caacggcttg
    17401 ggttggcgtc atttcactcc cacatggatg tcgggcatca acttcttttt cgaccacgat
    17461 cttagccgtt accactcccg cgccggcatt ggcgcggagt actggcgcga ctatctaaaa
    17521 ttaagcagta acggctattt gcgactgaca aactggcgca gcgcacctga actggacaac
    17581 gattatgaag cacgcccggc caatggctgg gatgtacgcg cagaaggctg gttacccgcc
    17641 tggccgcacc ttggcggtaa actggtctat gaacagtatt atggcgatga agtggccctg
    17701 ttcgataaag acgatcggca aagtaatcct catgccataa ccgctggact taactatacc
    17761 cccttcccgc tgatgacctt tagcgcggag caacgtcagg gtaaacaggg cgaaaatgac
    17821 acccgttttg ccgtcgattt tacctggcaa cctggcagcg caatgcagaa acagcttgac
    17881 ccgaatgaag tcgctgcacg gcgtagcctt gcaggcagcc gttatgatct ggtggatcgc
    17941 aacaacaaca tcgttctgga atatcgcaaa aaagaactgg tccgcctgac cctgacagac
    18001 cccgtgacag ggaagtcagg agaagtgaaa tcactggttt cgtcgctaca aaccaaatat
    18061 gccctgaaag gctataacgt cgaagccacc gctctggaag ctgccggtgg taaagtggtt
    18121 acaacgggta aagatattct ggttaccctg ccggcgtacc ggttcaccag tacgccagaa
    18181 accgataaca cctggccgat tgaagtcacc gctgaagatg tcaaaggcaa tttttcgaat
    18241 cgtgaacaga gcatggtagt cgttcaggca cctacgctaa gccagaaaga ttcctcggta
    18301 tcgttaagta ccctgacgtt gagcgcggat tcccattcaa ccgccacact gacttttatt
    18361 gctcatgatg cagcaggtaa tcctgttatc gggctggtgc tctcgacgcg tcacgaaggt
    18421 gttcaggaca tcaccctttc tgactggaaa gataatggtg acggaagcta tacccagatc
    18481 ctgaccacag gagcgatgtc tggcacgctg acgctgatgc cacagctgaa cggtgtggat
    18541 gcggctaaag cccccgccgt ggtgaatatc atttctgttt cgtcatcccg gactcactcg
    18601 tcaattaaga ttgataagga ccgttatctc tccgggaatc ctatcgaggt gacggtagaa
    18661 ctgagagatg aaaatgacaa acctgttaag gagcaaaaac agcaactgaa taccgcagtc
    18721 agcatcgaca acgtgaaacc tggtgtcact acagactgga aagaaaccgc agatggcgtc
    18781 tataaggcaa cctataccgc ctataccaaa ggcagtgggc ttactgcgaa gctgttaatg
    18841 caaaactgga atgaagattt gcataccgct ggatttatca tcgacgccaa cccgcagtca
    18901 gcgaaaattg cgacattatc tgccagcaat aatggtgtgc tcgccaatga gaatgcagca
    18961 aacaccgtct cggtcaatgt cgctgatgaa ggaagcaacc caatcaatga tcataccgtc
    19021 acgtttgtgg tattaagcgg atcggcaact tcctttaaca atcaaaacac cgcaaaaacg
    19081 gatgttaatg gtctggcgac ttttgatctg aaaagtagta agcaggaaga caacacggtt
    19141 gaagtcaccc ttgaaaatgg cgtgaaacaa acgttaatcg tcagttttgt cggcgactcg
    19201 agtaccgcgc aggttgatct gcagaagtcg aaaaatgaag tggtcgctga cggcaatgac
    19261 agtgccacaa tgaccgcgac agttcgggat gcaaaaggca acctgctcaa tgacgtcaag
    19321 gtcaccttca atgtcaattc agcagcagcg aaactgagcc aaaccgaagt gaatagccac
    19381 gacgggatcg ccacagctac gctgaccagt ttgaaaaatg gtgattatac ggttacggcc
    19441 tctgtgagct ctggttctca ggctaatcaa caggtgattt ttatcggtga tcaaagtact
    19501 gctgccctga ccctcagtgt gccttcaggt gatatcaccg tcaccaacac agctccgcta
    19561 catatgactg caaccttgca ggataaaaat ggcaacccac taaaagataa agaaatcacc
    19621 ttctctgtgc caaacgacgt cgcaagtcgg ttctcgatta gcaacagcgg aaaaggcatg
    19681 acggatagca acgggactgc aatcgcctcc ctgaccggca cgttagcggg cacgcatatg
    19741 atcacggctc gtctggctaa cagcaatgtc agcgatacac agccaatgac gtttgtggcg
    19801 gataaagaca gagcggttgt cgttctgcaa acatcgaaag cggaaatcat tgggaatggc
    19861 gtggatgaga cgactctgac agcaacagtt aaagatcctt ttgataacgt ggttaaaaat
    19921 ctttcagtag tcttccgcac ctcccccgca gacacgcaac tgagtctgaa cgcgcgtaat
    19981 actaatgaga acggtattgc tgaagttacc cttaagggca cggttttggg tgttcataca
    20041 gccgaagcca tactgcttaa cggcaacaga gatacgaaga tagtcaatat tgcgcccgat
    20101 gccagcaacg cgcaggtcac cctgaacatc cctgcacaac aggtggtgac gaataacagc
    20161 gacagcgtgc agctgacggc gacggtgaaa gatccgtcga atcatccggt ggcgggaata
    20221 acggtgaact tcaccatgcc acaggacgtg gcggcaaact ttacccttga aaataacggt
    20281 attgccatca ctcaggccaa tggcgaagcg catgtcaccc tcaaaggcaa aaaagcgggc
    20341 acgcatacgg ttaccgcaac gctgggtaac aataatgcca gcgatgcgca accagtcacc
    20401 ttcgtggcgg ataaggacag cgcggttgtc gttctgcaaa catcgaaagc ggaaatcatt
    20461 gggaatggcg tggatgagac gactctgacg gcaacagtga aagatccttt tgataacgca
    20521 gtaaaagatc taccggtcac cttcagtacc aaccccgcag atactcaact tagtcagagc
    20581 aaaagcaata ctaacgacag tggtgtggcc gaagttacct ttaagggcac ggttttgggt
    20641 gttcatacag ccgaagccac actgcctaac ggcaacaacg atacgaagac agtcaatatt
    20701 gcgcccgatg ccagcaacgc gcaggtcacg ctgaatatcc ctgctcaaca ggtggtgacg
    20761 aataacagcg acagcgtgca gctgacggca acggtgaaag atccgtcgaa tcatccggtg
    20821 gcgggaataa cggtgaactt caccatgcca caggacgtgg cggcaaattt taaccttgaa
    20881 aataacggta ttgccatcac tcaggccaat ggggaagcgc atgtcacgct gaaaggtaaa
    20941 aaagcgggta cgcatacggt taccgcaacg ctgagtaata acaataccag tgattcacag
    21001 ccggtaacgt ttgtggcgga caaaacctcg gctctggttg ttcttcagat atcaaaaaat
    21061 gagatcacag gtaatggcgt cgatagcgca acgctaactg caacggtcaa agatcagttc
    21121 gacaatgagg tgaacaatct tccggtaaca ttcagcacag cttcttcagg cctcaccctg
    21181 accccagggg aaagtaatac caatgagtct ggcatcgcgc aggccactct cgcaggcgtt
    21241 gcctttggtg agcagacggt cactgcatca ctggctaata atggtgccag cgacaacaaa
    21301 actgtgcatt ttattggcga cacagcggca gcaaaaatta tcgagttgac gcctgtccca
    21361 gacagcataa tcgccggtac cccgcagaac agctccggca gcgtcatcac cgccacagtc
    21421 gttgataata atggctttcc ggtgaaaggt gtgactgtga acttcaccag cagaacaaac
    21481 tctgccgaaa tgacgaatgg cggccaagcc gtaacgaacg aacagggtaa ggctaccatc
    21541 acttatacca atacccgctc ctcgatagaa tcaggagcga gaccggatac cgttgaggcc
    21601 agtctggaaa atggtagctc cacgcttagc acatcaatta atgtcaatgc tgatgcgtct
    21661 acggcacatc tcactttgct acatgcactt tttgatacag tctccgcagg cgagacaacc
    21721 agtctgtata ttgaggtgaa ggataattac ggcaacggtg tcccccagca tcaggtaacc
    21781 ctcagcattt caccaagtga aggcgtgacc ctcagtaata acggtatata tactaccaac
    21841 tactacggct atttttacgc aagctttacc gctacaaaag ccggggttta tcaagtgacg
    21901 gcaaccctcg ataatggcga ttcgatgcaa caaacagtga cctatgtgcc gaacgtcgcg
    21961 aatgctgaaa tcacgctggc agcctcgaag gatccggtga ttgccgacaa taacgatctc
    22021 acgacactaa cagcaacagt cgctgataca gagggcaatg cgatagccaa cactgaagta
    22081 acatttactc tgccggaaga tgtgaaggcg aacttcacgc tgagcgatgg cggtaaagcg
    22141 gttactgata ctgaaggcaa agcgaaagtc atgctgaaag gtacaaaagc aggcgctcat
    22201 actgttacag catcgatggc tggcggtaag agtgagcagt tggtggtgaa ttttattgcg
    22261 gatacgctca cagcgcaggt taatcttaac gttaccgagg acaattttat cgctaataac
    22321 gtcgggatga ccaggctgca ggcaacagtg actgatggaa acggcaaccc gttagccaat
    22381 gaggtggtga cattcacgct accggcagac gtgagtgcaa gcttcactct cggacaaggc
    22441 ggttccgcca ttactgatat caacggcaag gctgaagtta cactgagcgg tacaaaatcc
    22501 ggcacctatc ccgtgacagt tagcgtgaac aattatggtg tcagtgatac gaaacaggtg
    22561 actttgattg ccgatgctgg taccgctaaa atggctggtt ttaccgcatc atcaagctca
    22621 ttcaccgcga gcacaaccga gggcgcgacc ctgacggcaa gcgtcactga cgcttacggc
    22681 aacccgctag aaggtatcaa ggtcaatttc cgcggccccg ccaccacgct gagtaacacc
    22741 agtgtcgaaa cggatgctca gggtaaggca gaaatccttg tgacaagcac cattgctgga
    22801 acgaaagtag ttacagctaa tttggcaatc gcacctaccg aagccgccat tcgaatgtta
    22861 actgtcaacg cagatgttga ttctgcgacg atcaccagtc tggaaatgcc tgaaggtcag
    22921 gtaataatca gagaacccat agcggttaaa gcacacgtcg acgaccagtt tggcaatcca
    22981 gttgctgacc aacttgtgac attcagtgca gagccgtcaa gttttaacat ggtcattagt
    23041 caggatacgg tttctacgaa taggcagggt atagctgagg tcaccatgac gcctggaaga
    23101 tacggttcgt atactgtgaa agcatccctg gcgaatggct ccttttatga gaaggacctg
    23161 gtggtaatcg atctaagact aacactcacg tcatcaagtc cgcttatcgg cgtcaatgac
    23221 ccatcgggag caactctgac tgtaaggtta actcatgcaa acggggctcc attgagccat
    23281 gaactcgtta cctttagcgt aacgcccgaa ggtgcaacgt taagtagcca aacggcgaca
    23341 accaacactt caggtgaggc acaagtcgtt ctgaccagca ataaagtcgg tacatatgtg
    23401 gtgacagcat cgatccatag cggcgtaatt attcagacac agacaaccgt gaaggttact
    23461 ggcaacccaa gcacagcaca tgttgctagt tttatcgctg atccgtcgac tctcacggcc
    23521 aacaacagtg atatcagcac gttaaaggct actgttgaag atagcagtgg gaacctggtc
    23581 gaaggtgtca atgtaaactt cgtcttaaaa agcggctctg ccacattgac gtcattaaca
    23641 gcggtgaccg atcaaaacgg actcgcgaca acgagcgtga gaggagctat gacagggagc
    23701 gtcacggtaa gcgcagaaac gagctatggt ggagcgcaaa cagtagatat aacgctggtg
    23761 gcaggcccgg cagacgcttc gctgtccgtc cttaagaaca accggtcatc actgaaaggg
    23821 gactttaccg aaagtgctga actacacctt gttctgcacg atctatcagg ccatccgatc
    23881 aatgtttctg aagggatgga atttgtgcaa tcaggtacca acgtgcccta tgtgcaagtt
    23941 agtgcaattg actacagcaa aaatttctcc ggcgagtaca aagccactgt tacaggcggc
    24001 ggagagggta tcgcaacgct gatccccgtg ttgaatggtg ttcaccaggc tggtctgaat
    24061 accacgatag aattcattag cgcggaaacc agaccaatga ccggtacagt gtcagtgaat
    24121 ggtgcaaacc taccgacagc ttcattccct tcgcagggat ttaccggggc gtattatcag
    24181 ttgaataatg acaactttgc accaggaaaa acggcggctg attatgcgtt ttcaagcact
    24241 gcctcctggg taggcgttga tgctactggt aaagtgacat tcaaaaatga cggtgatagc
    24301 aatactgtag aaatcacagc aaccccaaga tcaggtggcg ctatttacca aacgcaagta
    24361 cgggtgaagg gctggtggaa agataacaat aatattatct taccattaag tcgtgctgaa
    24421 aattattgta ataacgagat cggcaacggt tacgcgattc ctggcgtgaa tcttttaagc
    24481 agtggagaaa accgacgcga aatagggtca ctgttcggtg agtggggaga tatggggcat
    24541 tatatggacg ctgattttta ttcagaaata tattggtctt ctaataccgc aggtggaggc
    24601 cgacaataca ttgtttccct ggaaaacggg gcgcatggct ctgttcaaac atctgaatat
    24661 tttcatgtgg catgttataa aaagagctaa tttttcgcta tattttctta cagagtgctc
    24721 cattcggggc gctctgcttt tacattcgtt tgctatcaaa cgctccatta atatacctgc
    24781 attttcccat acctccctgt tagtctgaag atagtcgagc ctcctttcgt ttttaataaa
    24841 cgctttcaca catggtataa ccacctgttc cacgccagcg ggaaacaaag tatatgatct
    24901 attttgataa cgatcattgt tgctatgtag tcaaattacg ttaatgttta gctaagtaca
    24961 tcaaggagga agatatgtca ggacgtacaa acaatatcag ttcatatttc aatattacaa
    25021 attacatcag taccactcat aataatgtat gcctgttaac ggggatttaa gttcaggttg
    25081 tcttatttat gatggtgaaa tacattacaa tgttttagtt ccaccagatt cgcgttcggt
    25141 gtgactatga tcgtatccgg tgagtacagc tttgatccaa aggctggttt tctgtgatga
    25201 ttgaccagcc tcagagtatt gatgacacca ccgttcataa cagatcggca ttactatgca
    25261 taagtactgt ctcatattct cgccaaaaca ctttttaatg cctttgcgaa ttcatccaga
    25321 cttatattgt caattttttg cccaacgacg actgactccg aaacatcggc cattgcgggc
    25381 catcgcgttt tgtaatcagc ataaattgca acaacggggg tattataggc acttgccata
    25441 tgtaatattg aggtgtcagg agttattgcg atataagcat cttttatgat tgctgctgaa
    25501 cgtttaatcg agggtgataa ggataaacgg tagacattat tataacaatc cactaatacc
    25561 ctggctttat cttccccttt tggtccatga acgatcacta ttggtatatc tgtttcactt
    25621 tgaatttttt ctattagatt ttctgcaatc gatagtgaaa atgtacgatc ctgcgagcta
    25681 ccttctaaat taagcgcaat gtaagatcct aacgatcgca tttcctcgcg cacctcatcg
    25741 agtacatcct cacttagtgg caattcatat attggcctga ctgccggaaa acccgcctca
    25801 cgcatcataa atgcccatgt catgggtacg ggagcccgga gattctgatc catacgggac
    25861 gcgttcttac acacagggga atagcacttc atggttaaac caacaacctg aaaattcgtt
    25921 tttgctttca actgactgat aaataacatc gttttcaggt tctttttacg catcgcctct
    25981 atacaaagat ccggcgtacc gtattgctgt gttatgttct tcgctaaatc ttttatttct
    26041 tttaatgttg cgtgatcctg catagtcatt gtgactaatg ttaatttagt ctgttcaagt
    26101 ttgagcgcat taaagacttc taaattaatt gtcgacgtaa caattagtag atgcttaatt
    26161 ttatgcaatt caagcgcccg aataacagga aagatggcca tagcatcgcc aatctgatcg
    26221 ggaatatgga tgacaacaaa atctgttttt tcaatattga aattataagc tttataatcg
    26281 tagtaactaa atgcaatacg tctcaacaat gatactaaaa acataactaa cctcgcctcc
    26341 ctactggtta taatgcaatg cagtctatca gaatcatcag ggtgcaattt tgtgcatatg
    26401 cggactttta tgtttcatat ctcgaacctg tggggcctct gcttaatcct taaacaacac
    26461 cagcaactcc tgcgctttca tcttccagcg aatttttcat gttgctgctc atcagccata
    26521 aaatcatttg cagatgcgct ctgtcgaggt agtctcataa ggttcgttta tagatcgacg
    26581 gcaatgtgag ttaccttttc catactaatt ataaaaagac agtacaaaca ggatcattat
    26641 ggactccacg ctcatctcca ctcgtcccga tgaagggacg ctttcgttaa gtcgcgcccg
    26701 acgagctgcg ttaggtagtt tcgctggtgc cgtcgtcgac tggtatgatt ttttactcta
    26761 tggcatcacc gccgcactgg tgtttaatcg cgagtttttc ccgcaagtaa gcccggcgat
    26821 gggaacgctc gccgcatttg ctacctttgg cgtcggattc cttttccgtc cgctcggcgg
    26881 tgtcattttc ggtcactttg gcgaccgact gggacgtaag cgcatgttaa tgctgaccgt
    26941 ctggatgatg ggcatcgcga cagccttgat tggtattctt ccttcattct cgaccattgg
    27001 gtggtgggca cctattttgc tggtgacact gcgtgccatt cagggatttg ccgtcggcgg
    27061 cgaatgggga ggcgcggcgt tgctttccgt tgaaagtgcg ccggaaaata aaaaagcctt
    27121 ttacagtagc ggtgtacaag ttggctacgg tgtaggttta ctgctttcaa ccggactggt
    27181 ttcattgatc agtatgatga cgactgacga acagttttta agctggggct ggcgtattcc
    27241 tttcctgttt agcatcgtac tggtactggg agcattgtgg gtgcgcaatg gcatggagga
    27301 gtccgcggaa ttcgaacaac agcaacataa tcaagcggcc gcgaaaaaac gcatcccggt
    27361 tatcgaagct ctgttacgac atcccggtgc tttcctgaag attattgcgc tacgactgtg
    27421 cgaattgctg acgatgtaca tcgttactgc ctttgcactt aattattcaa cccagaatat
    27481 ggggttaccg cgcgaacttt tccttaatat tggtttgctg gtaggtggat taagctgcct
    27541 gacaattccc tgttttgcct ggcttgccga tcgttttggt cgccgcaggg tttatatcac
    27601 aggcgcgttg atcggaacgt tgagcgcatt tcctttcttt atggcgcttg aagcacaatc
    27661 tattttctgg atagttttct tctccataat gctggcaaac attgcgcatg acatggtggt
    27721 gtgtgtgcaa caaccgatgt ttaccgaaat gtttggtgcc agttatcgct atagtggtgc
    27781 tggagtcggt tatcaggttg ccagtgtggt tggcggtgga tttacacctt ttattgccgc
    27841 tgcgctcatc acttactttg ccgggaactg gcatagcgtc gccatttatt tgctggctgg
    27901 atgtctgatt tccgcaatga ccgctttgtt gatgaaagac aatcaacgcg cttgatagtc
    27961 tggcgaagat catccgatct tcgccttaca cttttgtttc acattcctgt gacatactat
    28021 cggatgtgcg gtaattgtat ggaacaggag acacacatga ataataaggg ctccggtctg
    28081 accccagctc aggcactgga taaactcgac gcgctgtatg agcaatctgt agtcgcatta
    28141 cgcaacgcca ttggcaacta tattacaagt ggcgaattac ctgatgaaaa cgcccgcaaa
    28201 caaggtcttt ttgtctatcc atcactgacc gtaacctggg atggtagcac aaccaatccc
    28261 cctaaaacgc gcgcatttgg tcgttttact cacgcaggca gctacaccac cacgattact
    28321 cgccctactc tctttcgttc gtatctcaat gaacaactta cgttgctgta tcaggattat
    28381 ggtgcgcata tctcggtgca accctcgcag catgaaatcc cttatcctta tgtcatcgat
    28441 ggctctgaat tgacacttga tcgctcaatg agcgctgggt taactcgcta tttcccgaca
    28501 acagaactgg cgcaaattgg cgatgaaact gcagacggca tttatcatcc aactgaattc
    28561 tccccgctat cgcattttga tgcgcgccgc gtcgattttt ccctcgcgcg gttgcgccat
    28621 tataccggta cgccagttga acattttcag ccgttcgtct tgtttaccaa ctacacacgt
    28681 tatgtggatg aattcgttcg ttggggatgc agccagatcc tcgatcctga tagtccctac
    28741 attgcccttt cttgtgctgg cgggaactgg atcaccgccg aaaccgaagc gccagaagaa
    28801 gccatttccg accttgcatg gaaaaaacac cagatgcccg cgtggcattt aattaccgcc
    28861 gatggtcagg gtattactct ggtgaatatt ggcgtgggac cgtcaaatgc taaaaccatc
    28921 tgcgatcatc tggcagtact acgcccggat gtctggttga tgattggtca ctgtggtggc
    28981 ttacgtgaaa gccaggccat tggcgattat gtacttgcac atgcttattt acgcgatgat
    29041 cacgttcttg atgcggttct gccgcccgat attcctattc caagcattgc tgaagtgcaa
    29101 cgtgcgcttt atgacgccac caagctggtg agcggcaggc ccggtgagga agtcaaacag
    29161 cggctacgta ctggcactgt ggtaaccaca gatgacagga actgggaatt acgttactca
    29221 gcttctgcac ttcgttttaa cttaagccgg gccgtagcaa ttgatatgga aagtgcaacc
    29281 attgccgcgc aaggatatcg tttccgcgtg ccatacggga cactactgtg tgtttcagat
    29341 aaaccgttgc atggcgagat taaacttccc ggccaggcta accgttttta tgaaggtgct
    29401 atttccgaac acctgcaaat tggcattagg gcgatcgatt tgctgcgcgc agaaggcgac
    29461 cgactgcatt cacgtaaatt acgaaccttt aatgagccgc cgttccgata ataagttctg
    29521 aaccttctta tcaaaaatag atgtgaagga ttaagtaaga catctggcaa atagcccgca
    29581 ataaacgcgg gctgtttgct tccttgggcg gatacgagtt ttattatcgt cttaatgatt
    29641 tccacatatt aaaagcaagt atgctttcaa aaca
//
LOCUS       DAZFFG010000052        29581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFFG010000052 DAZFFG010000000
VERSION     DAZFFG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.052"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            399..1538
                     /gene="wza"
                     /locus_tag="ACHJ7J_002583"
     CDS             399..1538
                     /gene="wza"
                     /locus_tag="ACHJ7J_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO5000801.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKVYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1544..1987
                     /gene="wzb"
                     /locus_tag="ACHJ7J_002584"
     CDS             1544..1987
                     /gene="wzb"
                     /locus_tag="ACHJ7J_002584"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO5000802.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMCGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1990..4152
                     /gene="wzc"
                     /locus_tag="ACHJ7J_002585"
     CDS             1990..4152
                     /gene="wzc"
                     /locus_tag="ACHJ7J_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO5000803.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAHNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDIAASAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4245..5084
                     /gene="wcaA"
                     /locus_tag="ACHJ7J_002586"
     CDS             4245..5084
                     /gene="wcaA"
                     /locus_tag="ACHJ7J_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO5000804.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVSALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5087..5575
                     /gene="wcaB"
                     /locus_tag="ACHJ7J_002587"
     CDS             5087..5575
                     /gene="wcaB"
                     /locus_tag="ACHJ7J_002587"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO5000805.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5572..6789
                     /gene="wcaC"
                     /locus_tag="ACHJ7J_002588"
     CDS             5572..6789
                     /gene="wcaC"
                     /locus_tag="ACHJ7J_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO5000806.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6764..7981
                     /gene="wcaD"
                     /locus_tag="ACHJ7J_002589"
     CDS             6764..7981
                     /gene="wcaD"
                     /locus_tag="ACHJ7J_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO5000807.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7992..8738
                     /gene="wcaE"
                     /locus_tag="ACHJ7J_002590"
     CDS             7992..8738
                     /gene="wcaE"
                     /locus_tag="ACHJ7J_002590"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO5000808.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VGKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVPGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8754..9302
                     /gene="wcaF"
                     /locus_tag="ACHJ7J_002591"
     CDS             8754..9302
                     /gene="wcaF"
                     /locus_tag="ACHJ7J_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO5000809.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9329..10450
                     /gene="gmd"
                     /locus_tag="ACHJ7J_002592"
     CDS             9329..10450
                     /gene="gmd"
                     /locus_tag="ACHJ7J_002592"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO5000810.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10453..11418
                     /gene="fcl"
                     /locus_tag="ACHJ7J_002593"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10453..11418
                     /gene="fcl"
                     /locus_tag="ACHJ7J_002593"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO5000811.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMIIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11421..11900
                     /gene="gmm"
                     /locus_tag="ACHJ7J_002594"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11421..11900
                     /gene="gmm"
                     /locus_tag="ACHJ7J_002594"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO5000812.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11897..13120
                     /gene="wcaI"
                     /locus_tag="ACHJ7J_002595"
     CDS             11897..13120
                     /gene="wcaI"
                     /locus_tag="ACHJ7J_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO5000813.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAESVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     VVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13123..14559
                     /gene="cpsB"
                     /locus_tag="ACHJ7J_002596"
     CDS             13123..14559
                     /gene="cpsB"
                     /locus_tag="ACHJ7J_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO5000814.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIMPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDIFDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14752..16122
                     /gene="cpsG"
                     /locus_tag="ACHJ7J_002597"
                     /gene_synonym="manB"
     CDS             14752..16122
                     /gene="cpsG"
                     /locus_tag="ACHJ7J_002597"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO5000815.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16177..17571
                     /gene="wcaJ"
                     /locus_tag="ACHJ7J_002598"
     CDS             16177..17571
                     /gene="wcaJ"
                     /locus_tag="ACHJ7J_002598"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO5000816.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLLVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRIEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17573..19051
                     /gene="wzxC"
                     /locus_tag="ACHJ7J_002599"
     CDS             17573..19051
                     /gene="wzxC"
                     /locus_tag="ACHJ7J_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO5000817.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHSLVVEVKRQFCRSEKMKMLLRAG"
     gene            19423..20703
                     /gene="wcaK"
                     /locus_tag="ACHJ7J_002600"
     CDS             19423..20703
                     /gene="wcaK"
                     /locus_tag="ACHJ7J_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO5000818.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20700..21920
                     /gene="wcaL"
                     /locus_tag="ACHJ7J_002601"
     CDS             20700..21920
                     /gene="wcaL"
                     /locus_tag="ACHJ7J_002601"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO5000819.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKSAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21931..23325
                     /gene="wcaM"
                     /locus_tag="ACHJ7J_002602"
     CDS             21931..23325
                     /gene="wcaM"
                     /locus_tag="ACHJ7J_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO5000820.1"
                     /translation="MPFKTLSRRTFLTASSALVFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNKYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGIFMAKEETLLSFANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23483..24478
                     /locus_tag="ACHJ7J_002603"
     CDS             23483..24478
                     /locus_tag="ACHJ7J_002603"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO5000821.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24721..25614
                     /gene="galF"
                     /locus_tag="ACHJ7J_002604"
     CDS             24721..25614
                     /gene="galF"
                     /locus_tag="ACHJ7J_002604"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO5000822.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDVMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            25983..27068
                     /gene="rfbB"
                     /locus_tag="ACHJ7J_002605"
     CDS             25983..27068
                     /gene="rfbB"
                     /locus_tag="ACHJ7J_002605"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5000823.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMALIFAQHQPDAVMHLAAESHVDRSIAGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LANAKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            27068..27967
                     /gene="rfbD"
                     /locus_tag="ACHJ7J_002606"
     CDS             27068..27967
                     /gene="rfbD"
                     /locus_tag="ACHJ7J_002606"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO5000824.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELEVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEACKVGIPLALNKLNAVPTSAYHTPACRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            28025..28903
                     /gene="rfbA"
                     /locus_tag="ACHJ7J_002607"
     CDS             28025..28903
                     /gene="rfbA"
                     /locus_tag="ACHJ7J_002607"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5000825.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFISG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFINAEQVQVLAESL
                     KKNAYGQYLLKMVEGY"
     gene            28908..29468
                     /gene="rfbC"
                     /locus_tag="ACHJ7J_002608"
     CDS             28908..29468
                     /gene="rfbC"
                     /locus_tag="ACHJ7J_002608"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO5000826.1"
                     /translation="MKVIKTSIPDVLIFEPKVYGDERGFFMESFNQKVFDEAVGRKIE
                     FVQDNHSQSSKGVLRGLHYQLEPYAQGKLVRCVEGEVFDVAVDIRKSSPTFGQWVGVY
                     LNAKNKRQLWIPEGFAHGFIVTSEKATFLYKATNYYMPSHEGGIIWNDKSLNIQWPLM
                     REMIISAKDQQQISLTEYLEIESSKK"
BASE COUNT         7472 a         7115 c         7889 g         7105 t
ORIGIN      
        1 agccaattgc cgaattgtta ccttgcctgc tattccgtta gctgtaacac ttcctcctgc
       61 attattggag agccaatatt caattaccat aatgtcacct tatattcgga agttaatttc
      121 cgcaataacc ctttctcatc aacgactgta tgttgagagg gttttaaaaa ttaagctaaa
      181 tcagtgtatt ggtagctaaa aagccagggg cggtagcgtg tctggatgcc tgaaagacca
      241 gtctgaatta ttctgtcaat agcctgcgga tgaaatcaat ttttttagga ctgatgccag
      301 ttaaattttt attcaattaa ctgcaaggta aatgtgcatg gcgacattgc tattaatagt
      361 gcacaggata attactctgc caaagtgata aataaacaat gatgaaatcc aaaatgaaat
      421 tgatgccatt attggtgtca gtaaccttga taagcggttg cacagtactt ccgggcagca
      481 atatgtcgac gatgggtaaa gacgtcatca aacagcagga cgctgatttc gatctcgaca
      541 aaatggtgaa tgtttatccg ctgaccccgc gcctgattga ccaattacgc ccacgcccga
      601 atgtagcgcg ccccaatatg acgctggaaa gcgagatcgc gaattaccag tatcgcgtcg
      661 ggccggggga cgttcttaat gtcaccgtct gggatcaccc ggaactcacc acgccagccg
      721 gtcagtaccg cagctccagc gacaccggca actgggtaca gcctgacggc accatgttct
      781 acccgtatat cggcaaggtt cacgtagtcg ggaaaacgct cgctgaaatc cgcagtgata
      841 ttaccgggcg cttagcgacg tacatcgctg acccgcaggt ggacgttaat atcgccgcct
      901 tccgctcaca aaaggtctat atctccggtc aggtgaataa atccggtcaa caggcgatca
      961 ccaacgtgcc actgactatt ctcgacgcca tcaacgccgc aggtggcctg accgacaccg
     1021 ctgactggcg caacgtggtg ctaacacaca atggtcgtga agagcgcatt tctttgcagg
     1081 cgctgatgca aaacggcgac ctcaaccaga atcgactgct ttaccccggc gatattctct
     1141 acgtgccgcg taatgatgat ctgaaagtat ttgtgatggg tgaagtgaag aaacagagca
     1201 ccctgaaaat ggactttagc ggcatgaccc tgactgaagc tctgggcaat gctgaaggca
     1261 ttgacatgac cacctccaac gccagcggca tctttgtcat tcgtccgctg aaaggcgagg
     1321 gcgggcgtaa cggcaagatt gccaatatct accagctgga tatgtccgat gccacgtcgc
     1381 tggtgatggc gacagaattc cgcctgcaac cttatgacgt ggtgtatgtc accaccgccc
     1441 cggtttcccg ctggaaccgt ctgatcaatc agttgctgcc aactattagc ggtgtccgtt
     1501 acatgacgga tacagccagc gacattcata actggtaatc gtcatgttta acaacatctt
     1561 agttgtctgt gtcggcaata tttgccgttc cccgacggcg gaacgcttac tgcaacgtta
     1621 tcacccggag ctgaaagtgg agtccgctgg actcggcgcg ctggtcggta agggagctga
     1681 tcctaccgcc atcagcgttg ccgcagagca tcaactgtct ctggaaggtc actgtgcacg
     1741 gcaaatcagc cgccgcctgt gtcgcaacta tgacctgatt ttgaccatgg aaaagcgcca
     1801 tatcgaacgc ttatgcgaga tggcacccga gatgtgcggc aaagtgatgc tgtttggtca
     1861 ctgggataac gaatgtgaaa tccccgatcc gtatcgcaaa agccgggaaa cgtttgcagc
     1921 ggtgtacaca ttacttgaac ggtctgcccg ccagtgggcg caggcattga acgcagagca
     1981 ggtataagaa tgacagaaaa agtaaaacaa catgccgctc cggtaacggg cagtgatgaa
     2041 atcgatattg gtcgcctggt cggcaccgtc attgaagcgc gctggtgggt gattggcatc
     2101 accgctgtat tcgccctttg tgccgtggtt tacaccttct tcgccacgcc gatttatagt
     2161 gccgacgcac tggtacaaat cgagcaaagc agcggcaatt cgttagtgca ggatatcgga
     2221 tcggcgttag ccaacaaacc gcctgcatcg gacgccgaga tccagttgat tcgttcgcgc
     2281 ctggtgcttg gtaaaacggt ggacgatctc gacctcgata ttgcagtgag caaaaacacg
     2341 ttcccgattt tcggggcggg ctgggatcgc ctgatgggac ggcagaacga gacggtgaaa
     2401 gtgactacct ttaaccgtcc gaaagagatg gcggatcagg tgtttacgct taatgtgctg
     2461 gatgacaaaa actacaccct gagcagtgat ggcggcttta gcgcccgtgg acaagcgggc
     2521 cagatgctga aaaaagaagg cgtcacgctg atggttgaag ccattcacgc ccgcccgggc
     2581 agtgagttta ccgtcaccaa atactccacg ctggggatga tcaaccaact gcaaaacagc
     2641 ctgacggtaa cggagaacgg caaagacgca ggcgtactga gcctgactta taccggtgaa
     2701 gatcgcgaac agatccgcga cattcttaac agcatcgccc gtaactatca ggaacaaaat
     2761 attgagcgca aatcggcgga agcgtcgaaa agcctcgctt tcctcgcgca acagttaccg
     2821 gaagtacgta gccgccttga tgttgccgaa aacaaactga atgccttccg tcaggataaa
     2881 gattctgttg atctgccgct ggaagcgaaa gcggtgctcg attcgatggt gaacatcgac
     2941 gcgcagttga acgaactgac ctttaaagag gcggaaatct ccaagctgta caccaaagtt
     3001 cacccggcgt accgcacgct gctggagaaa cgtcaggcgc tggaagacga aaaagccaaa
     3061 cttaatggtc gcgtaacggc gatgccgaaa acccagcagg agattgtgcg cctgacccgc
     3121 gatgtcgagt ctggtcagca ggtctatatg caactgctga ataaagagca ggagctgaaa
     3181 atcaccgaag ccagcaccgt cggcgatgtg cgcattgttg acccggcaat cacccagcct
     3241 ggtgtgctga aaccgaagaa agggttgatt atcctcggcg caattatcct cggcctgatg
     3301 ctctctatcg tgggtgtact gctgcgctcg ttgtttaatc gcggcatcga aagcccgcaa
     3361 gtgctggaag aacacggaat cagcgtctat gccagcatcc cgctgtcgga gtggcagaaa
     3421 gcgcgcgata gcgtcaaaac catcaaaggg gttaaacgct ataaacagag tcagctactg
     3481 gcggtgggga atccaaccga cctggcgatt gaagccatcc gcagtctgcg taccagtctg
     3541 cacttcgcga tgatgcaggc gcacaacaat gtgttgatga tgacaggggt tagcccgtca
     3601 atcggtaaaa cctttgtctg cgctaacctg gcggcggtca ttagccagac taataaacgc
     3661 gtattgttga tcgactgcga tatgcgcaaa ggctacaccc acgagctgtt gggcaccaat
     3721 aacgttaatg gcctgtcgga aattttgatt ggacagggcg atattgccgc cagcgccaaa
     3781 cctacttcga ttgccaaatt tgacctgatc ccgcgcggtc aggtaccgcc aaatccttct
     3841 gaactgttaa tgagcgaacg ctttgccgaa ctggtgagct gggcgagtaa aaactacgac
     3901 ctggtgttga ttgatacgcc gccgattctg gcagtgaccg atgcggcaat tgttggtcgt
     3961 catgccggaa ccacgttgat ggtggcgcgt tatgcggtca acaccttgaa agaagtggaa
     4021 accagtctga gccgctttga gcaaaacggt attccggtga aaggggtgat tctgaactcc
     4081 atcttccgcc gcgccagcgc gtatcaggat tatggctatt acgaatacga atataagtcg
     4141 gatgcgaaat aacgacgcct gcattcgcac cgccccgtag gccggataag gcgctcacgc
     4201 cacatccggc aagcaaacca gctcataagc cgggagaaca acctatgaaa gacaatccgc
     4261 tgatctcaat ctatatgccg acctggaacc gccaacaact ggcgattcgg gcgataaaat
     4321 cggtcttgcg ccaggactac agcaactggg agatgatcat cgtggatgat tgctccacct
     4381 cctgggaaca gcttcagcag tacgtcagcg ccctcaacga tccgcgtatt acctacattc
     4441 acaacgacat taacagcggg gcgtgcgcgg tacgtaacca ggctattatg ctggcgcaag
     4501 gggaatacat taccgggatc gatgacgatg acgaatggac acccaaccgt ctgagcgtct
     4561 tcctcgccca taaacagcaa ctggtcactc acgccttttt gtacgctaac gactacgttt
     4621 gtcagggcga agtctattcc caaccggcaa gtttgccgct gtatcccaaa tcgccgtatt
     4681 cacgccgcct gttctataaa cgcaatatca ttggcaatca ggtctttacc tgggcgtggc
     4741 gtttcaaaga gtgtttgttc gataccgaac tgaaagcggc acaggattac gacattttcc
     4801 tgcggatggt ggtggagtac ggcgaaccgt ggaaagtgga agaggcgacg cagatcctgc
     4861 atatcaatca cggcgaaatg cagatcacct cgtcaccgaa aaaattctcc gggtacttcc
     4921 acttttaccg caagcacaaa gacaaattcg accgtgccag caaaaaatat cagctgttta
     4981 ctctctacca gatccgcaat aaacgtatga cctggcgcac tttgctgacg ctgctgtcgg
     5041 tgcgtaacgg caaacgtctg gctgatggta ttcgggggcg ctaaatatgc tggaagatct
     5101 acgcgccaac agctggagtt tacgcccgtg ctgcatggtt cttgcctatc gcgtcgctca
     5161 tttttgttcg gtgtggcgca aaaagaacgt cctcaacaat ctgtgggcgg ccccgctgct
     5221 ggtgctgtat cgcattatca ccgaatgctt tttcggttat gaaatccagg ccgccgcgac
     5281 cattggccgc cgctttacta tccatcacgg ttacgccgta gtgatcaata aaaacgtggt
     5341 ggcgggggat gatttcacca ttcgccacgg cgtcactatc ggcaatcgtg gtgccgataa
     5401 catggcatgt ccacacattg gcaacggcgt cgaactcggt gccaacgtca ttattcttgg
     5461 tgatatcacg cttggtaaca acgtcaccgt gggcgcgggc agcgtggtgc tcgactctgt
     5521 cccggacaac gcgctggtgg tgggagaaaa agcgcgagtg aaggtaatta aatgaatatt
     5581 ctgcaattta atgtgcgact ggcggaaggc ggggcagcag gtgtggcgtt agatctccac
     5641 cagcgcgcgc tgcaacaggg gctggcgtca cattttgtct acggctacgg caaaggcggc
     5701 aaagagagcg tcagccatca gaactatccg caggtaatca aacatacgcc gcggatgacc
     5761 gcaatggcga atattgccct gtttcgtctg tttaatcgcg atctgtttgg caatttcaat
     5821 gagttatatc gcaccattac tcgtacaccg ggtccggtgg tcctgcattt tcatgtgctg
     5881 cacagctact ggctaaatct taagagcgtg gtgcgctttt gcgaaaaggt gaaaaaccat
     5941 aaaccggacg tcactctggt ctggacgctg cacgaccact ggagtgtaac cggacgctgc
     6001 gcctttaccg acggttgcga aggctggaaa acgggctgcc agaaatgccc gaccttaaat
     6061 aattatccgc cggtgaagat tgatcgcgca caccaactgg tggcgggcaa acgccagtta
     6121 ttccgtgaga tgctggcgct gggctgtcag tttatttccc ccagccagca tgtggctgac
     6181 gctttcaata gcctgtacgg tccagggcgt tgccggatta tcaataatgg tattgatatg
     6241 gcaaccgaag cgattctggc ggatctgcct ccggtgcgcg aaactcaggg caagccgaaa
     6301 atcgcggtgg tggcgcatga cctgcgttac gacggtaaga ctaaccagca actggtgcgt
     6361 gagatgatgg cgctgggcga caaaattgaa ctgcatacct ttggtaagtt ctcgccgttc
     6421 accgctggca acgtggttaa tcacggcttt gaaactgaca agcgcaagtt gatgagcgcg
     6481 ctcaatcaga tggatgcgct ggtattcagt tctcgcgtcg ataactaccc actgattttg
     6541 tgtgaggcgc tatcgattgg tgtgccggtg attgccaccc atagcgacgc ggcgcgtgaa
     6601 gtgttgcaaa aatccggcgg taaaaccgtc agcgaagaag acgtgctgca actggtccag
     6661 ttaagcaaac cggaaatcgc gcaggcgata tttggtacca cgctggctga gttcagccaa
     6721 cgcagccgcg cggcctacag tggacaacag atgctggagg agtatgtcaa cttctatcag
     6781 aatctgtagc tacctgctgc tgccgctgat ctacctgctg gttaacgtca aaatcgccca
     6841 gcttggagaa agtttcccca tcaccatcgt cactttttta cctgtcttgt tactgctgtt
     6901 tttagaacgc atcagcgtta aaaaattgat gattgcctta gggattggcg cgggactcac
     6961 ggcgtttaac tacctgtttg gtcagtcgtt ggatgccagt aaatacgtca cttcaaccat
     7021 gctgtttgtc tatattgtga tcattattgg catggtgtgg agtattcgct ttaaaacgat
     7081 ttcgccacac aaccatcgca agattttacg tttcttttat ctggtggtcg ggctggtggt
     7141 ggcgctggcg gcggtggaga tggcgcaaat tatcctcacc ggtggcagca gtattatgga
     7201 gtcgatttcg aaatatctga tttacagcaa cagctatgtg ctgaatttca ttaaattcgg
     7261 cggcaagcgc acgacagcac tttatttcga accggcattt ttcgctctgg cattaatctc
     7321 aatttggctc agcatcaaac agtttggtat caaaacgcct aaaaccgatg ctatgattct
     7381 cgcagggata atattatccg gatcgttttc aggggttatg acctttatcc tgttttattt
     7441 gctggagtgg gcatttcaat atctgaataa agaggcgatt aagaaaaagt taccgttagc
     7501 attgatttct ctggctgtat tcctggttgg tgtggtaatc gcgtttcctt atatttccac
     7561 ccgtctgggc gatttaggta cggaaggatc gtcatcgtat tatcgtattg tcggtccgct
     7621 ggtgatggtc ggttattctt tgacccatat tgacggtgta gtcagatttg gctcacttta
     7681 tgaatatgtc gcatcattcg gaatatttaa cggtgcggat gtcggaaaaa ccatagacaa
     7741 tggtttgtat ctgctgatta tttatttttc ctggttcgcg gtgtttttat cgctgtggta
     7801 catggggaaa gtgataaaaa tgatgatcaa cgcttttggt gataaccgca attttcgcgt
     7861 gcaattatat ctttttactc cggtatcgct gttttttacc ggttcgatat ttagcccgga
     7921 atatgcattt ttaatcgtct gtccgtttat tttgcgaaaa gcgttaaata ttacgaggta
     7981 agaataagaa catgttgctt agcataatca ctgtcgcgtt tcgtaacctc gaagggatag
     8041 tcaaaacaca cgcctcgctg gcgcatctgg cgcaggcgga cgatatcagc ttcgaatgga
     8101 ttgttgtcga tggcggttcc aatgacggca cccgtgagta cctggaaaat ctcaatggta
     8161 tctataacct acgctttgtc agcgagccag ataacggtat ctacgacgcc atgaacaaag
     8221 gcattgcgat ggcacaaggc aagttcgcgt tgtttctcaa ctcgggcgat atttttcatc
     8281 aggatgccgc aaattttgtc ggtaagttaa aaatgcaaaa agataacgtg atgatcaccg
     8341 gcgatgcgct gctggatttt ggcgacgggc ataaaattaa acgtagcgcc aaaccaggtt
     8401 ggtatattta tcacagcctg cccgccagtc atcaggcgat atttttcccg gtacccggct
     8461 tgaaaaaatg gcgttatgac ctggaatata aagtttcttc cgattacgcg ctggcagcca
     8521 aaatgtataa agccggttat gcatttaaaa aactcaatgg cctggtgtct gaattttcta
     8581 tgggtggggt atcaaccacc aataatatgg aattgtgtgc tgacgccaaa aaagtccaac
     8641 ggcaaatatt acatgtgcct ggcttctggg cagaattatc ctggcattta cgccaacgta
     8701 ctacctcaaa gacgaaagcc ttatataaca aaagctgaat ataagggaaa acgatgcaag
     8761 atttaagtgg attctcggtg ccgaaagggt tccggggcgg caacgctatt aaagtgcaat
     8821 tatggtgggc agtacaggca acaatatttg cctggtcgcc acaagtattg tatcgctggc
     8881 gggctttttt attacgttta ttcggcgcaa aaataggaaa aaacgtagtt attcgtccgt
     8941 cagtaaaaat tacctatccg tggaaattaa ccttaggtga ttacgcgtgg gtcggcgatg
     9001 acgtcaattt atataccctc ggtgaaataa ccattggcgc acattcggtg atatcgcaaa
     9061 aaagttattt atgcaccggt agccacgacc atgcaagtca acatttcacc attaacgcca
     9121 cgcctattgt gattggcgag aaatgctggc tggcgacaga tgtttttgtt gcccctggcg
     9181 tcacgattgg cgacggcacc gtcgtgggtg cacgaagcag tgtttttaaa tcgcttccgg
     9241 caaatgtggt ttgccggggg aatcccgcag tggtgatacg cgaacgcgtt gaaactgaat
     9301 aaattcaaaa aatacagagg aataagacat gtcaaaagtc gctctcatca ccggtgtaac
     9361 cggacaagac ggttcttacc tggcagagtt tctgctggaa aaaggttacg aggtgcatgg
     9421 tattaagcgt cgcgcatcgt cattcaacac cgagcgcgtg gatcacattt atcaggatcc
     9481 gcacacctgc aacccgaaat tccatctgca ttatggcgac ctgagtgata cctccaacct
     9541 gacgcgcatt ttgcgtgaag tacagccgga tgaagtgtac aacctggggg caatgagcca
     9601 cgttgcggtc tcttttgagt caccggaata taccgctgac gtcgacgcga tgggtacgct
     9661 gcgcctgctc gaggcgatcc gcttcctcgg tctggaaaag aaaacccgtt tctatcaggc
     9721 ttccacctct gaactgtatg gtctggtgca ggaaattccg cagaaagaaa ccacgccgtt
     9781 ctacccgcga tctccgtatg cggtcgccaa actgtacgcc tactggatca ccgttaacta
     9841 ccgcgaatcc tacggcatgt acgcctgcaa cggtattctc ttcaaccatg aatccccgcg
     9901 ccgtggcgaa accttcgtta cccgcaaaat cacccgcgca atcgccaaca tcgcccaggg
     9961 gctggagtcg tgcctgtacc tcggcaatat ggattccctg cgtgactggg gccatgccaa
    10021 agactacgta aaaatgcagt ggatgatgct gcaacaggaa caaccggaag atttcgttat
    10081 tgctaccggc gttcagtact ccgtacgtca gttcgtggaa atggcggcgg cacagctggg
    10141 catcaaactg cgctttgaag gcacgggcgt tgaagagaag ggcattgtgg tttccgtcac
    10201 cggacatgac gcgccgggcg ttaaaccggg tgatgtgatt atcgccgttg acccgcgtta
    10261 cttccgtccg gctgaagttg aaacgctgct cggcgacccg accaaagcgc acgaaaaact
    10321 gggctggaaa ccggaaatca ccctcagaga gatggtgtct gaaatggtgg ctaatgacct
    10381 cgaagcggcg aaaaaacact ctctgctgaa atctcacggc tacgacgtgg cgatcgcgct
    10441 ggagtcataa gcatgagtag acaacgcatt tttatcgctg gccatcgcgg gatggtcggt
    10501 tctgccatca ggcggcagct cgaacagcgc ggtgatgtgg aactggtatt acgcacccgc
    10561 gacgagctga acctgctgga cagccgcgcc gtgcatgatt tctttgccag cgaacgtatt
    10621 gaccaggtct atctggcggc cgcgaaagtg ggcggcattg ttgccaacaa cacgtatccg
    10681 gcggatttca tctatcagaa catgataatt gagagcaaca tcattcacgc cgcgcatcag
    10741 aacgacgtga acaaactgct gtttctcgga tcgtcctgca tctacccgaa actggcaaaa
    10801 cagccgatgg cagaaagcga gttattgcag ggcacgctgg agccgaccaa cgagccttac
    10861 gccattgcca aaatcgccgg gatcaaactg tgcgaatctt acaaccgcca gtatggacgc
    10921 gattaccgct cagtcatgcc gaccaacctg tatggcccgc atgacaactt ccacccgagt
    10981 aattcgcatg tgatccctgc attgctgcgt cgcttccacg aggcgacggc acagaatgca
    11041 ccggacgtgg tggtatgggg cagcggtaca ccgatgcgtg aattcctgca cgtcgatgat
    11101 atggcggcgg cgagcattca tgtcatggag ctggcgcatg aagtctggct ggagaacacc
    11161 cagccgatgc tgtcgcacat taacgtcggc acgggcgttg actgcactat ccgcgagctg
    11221 gcgcaaacca tcgccaaagt ggtgggttac aaaggtcggg tggtttttga tgccagcaag
    11281 ccggatggta cgccgcgcaa actgctggat gtgacgcgcc tgcatcagct tggctggtat
    11341 cacgaaatct cactggaagc ggggcttgcc agcacttacc agtggttcct tgagaatcaa
    11401 gaccgctttc gggggtaatg atgtttttac gtcaggaaga ctttgccacg gtagtgcgct
    11461 ccactccgct tgtctctctc gactttattg tcgagaacag tcgcggcgag tttctgcttg
    11521 gcaaaagaac caaccgcccg gcgcagggtt actggtttgt gccgggaggg cgcgtgcaga
    11581 aagacgaaac gctggaagcc gcatttgagc ggctgacgat ggcggaactg gggctgcgtc
    11641 tgccgataac agcaggccag ttttacggtg tctggcagca cttttatgac gataacttct
    11701 ctggcacgga tttcaccact cactatgtgg tgctcggttt tcgcttcaga gtagcggaag
    11761 aagaactgtt actgccggat gagcagcatg acgattaccg ctggctgacg ccggacgcac
    11821 tgctcgccag tgacaatgtt cacgctaaca gccgcgccta ttttctcgct gagaagcgtg
    11881 ccggagtacc cggattatga aaatactggt ctacggcatt aactactcgc cggagttaac
    11941 cggcatcggc aaatacaccg gcgagatggt ggaatggctg gcggcacaag gtcatgaggt
    12001 gcgggtcatt accgcaccgc cttactaccc gcagtggcag gtgggcgaga actattccgc
    12061 ctggcgctac aaacgagaag agggggccgc cacggtgtgg cgctgcccgc tgtatgtgcc
    12121 aaaacagccg agcaccctga aacgcctgtt acatctcggc agttttgccg tcagcagttt
    12181 tttcccgttg atggcgcaac gtcgctggaa gccggatcgc attatcggcg tggtgccaac
    12241 gctgttttgc acgccgggaa tgcgcctgct agcgaaactc tctggtgcgc gtaccgtgct
    12301 gcatattcag gattacgaag tggacgccat gctggggctg ggccttgccg gaaaaggcaa
    12361 aggcggcaaa gtggcacagc tggcgacggc gttcgaacgt agcggactgc ataacgtcga
    12421 taacgtctcc acgatttcgc gttcgatgat gaataaagcc atcgaaaaag gcgtggcggc
    12481 ggaaagcgtc atcttcttcc ccaactggtc ggaaatcgcc cgttttcagc atgttgcaga
    12541 cgccgatgtt gacgcccttc gtaaccagct tggcctgccg gataacaaaa aaatcattct
    12601 ttactccggc aatattggtg aaaagcaggg gctggaaaac gttattgaag ctgccgatcg
    12661 cctgcgcgat gaaccgctga tttttgccat tgtcgggcag ggcggcggca aagcgcggct
    12721 ggaaaaaatg gcgcaacagc gtggtctgcg caacatgcaa ttcttcccgc tgcaatcgta
    12781 tgacgcttta cccgcactgc tgaagatggg cgattgccat ctggtggtgc aaaaacgcgg
    12841 cgcggcagat gccgtattgc cgtcgaaact gaccaatatt ctggcggtag gcggtaacgt
    12901 ggtgattact gcagaagccc acacagaact gggacagctt tgcgaaacct ttccgggcat
    12961 tgcggtttgc gtagaaccgg aatcggtcga ggcgctggtg gcggggatcc gtcaggcgct
    13021 cctgctgccc aaacacaaca cggtggcacg tgaatatgcc gaacgcacgc tcgataaaga
    13081 gaacgtgtta cgtcaattta taaatgatat tcggggataa ttatggcgca gtcgaaactc
    13141 tatccagttg tgatggcagg tggctccggt agccgcttat ggccgctttc ccgcgtactt
    13201 taccccaagc agtttttatg cctgaaaggc gatctcacca tgctgcaaac caccatctgc
    13261 cgcctgaacg gcgtggagtg cgaaagcccg gtggtgattt gcaatgagca gcaccgcttt
    13321 attgtcgcgg aacagctgcg tcaactgaac aaactcaccg agaacattat tctcgaaccg
    13381 gcagggcgta acactgcacc agccattgca ctggcggcgc tggcggcaaa acgtcatagt
    13441 ccggagagcg acccgttaat gctggtattg gcggcggatc atgtgattgc cgatgaagac
    13501 gcgttccgtg ccgccgtgcg taatgccatg ccgtatgccg aagcgggcaa gctggtgacc
    13561 tttggcatta tgccggatct acctgaaacc ggttatggct atattcgtcg cggtgaagtg
    13621 tcggcgggtg agcaggatac ggtggccttt gaagtggcgc agtttgtcga aaaaccaaat
    13681 ctggaaaccg cgcaggccta tgtggcaagc ggcgaatatt actggaacag cggtatgttc
    13741 ctgttccgtg ccggacgcta tctcgaagaa ctgaaaaaat atcgcccgga tattttcgat
    13801 gcctgtgaaa aagcgatgag cgccgtcgat ccggatctcg attttattcg cgtggatgaa
    13861 gaagcgtttc tggcctgccc ggaagagtct gtggattacg cggtcatgga acgcacggca
    13921 gatgccgttg tggtgccgat ggatgcgggc tggagcgatg tcggttcctg gtcttcatta
    13981 tgggagatca gcgcccacac cgccgagggc aacgtttgcc acggcgatgt gattaatcac
    14041 aaaactgaaa acagctatgt gtacgccgaa tctggcctgg tcaccaccgt cggggtgaaa
    14101 gatttggtgg tagtgcagac caaagatgcg gtgctgattg ccgaccgtaa cgcggtgcag
    14161 gatgtgaaaa aagtggtcga gcagatcaaa gccgatggtc gccatgagca tcgggtacat
    14221 cgcgaagtgt atcgtccgtg gggcaaatat gactctatcg acgcgggcga ccgctaccag
    14281 gtgaaacgca tcaccgtgaa accgggcgaa ggcttgtcgg tacagatgca ccatcaccgt
    14341 gcggaacact gggtagtggt cgcgggaacg gcaaaagtca ctattgacgg tgatatcaaa
    14401 ctgcttggtg aaaacgagtc catttatatt ccgctggggg cgacgcactg cctggaaaac
    14461 ccggggaaaa ttccgctcga tttaattgaa gtgcgctccg gctcttatct cgaagaggat
    14521 gatgtggtgc gcttcgcgga tcgctacgga cgggtgtaaa cgtcgcatca ggcgctgttt
    14581 gtcggatgcg gcgtgaacgc cttatccgac ctacggttcg gttttgtagg cctgataaga
    14641 cgcggcagcg tcgcatcagg caatgaatgc gaaaccgcgg tgtaaataac gacaaaaata
    14701 aaattggccg tttcggtcag ggccaactat tgcctgaaaa agggtaacga tatgaaaaaa
    14761 ttaacctgct ttaaagccta cgatattcgc gggaaattag gcgaagaact gaatgaagat
    14821 atcgcctggc gcattggtcg cgcctatggc gaatttctta aaccgaaaac cattgtttta
    14881 ggcggtgatg tccgtctcac cagcgaaacc ttaaaactgg cgctagcgaa aggtttacag
    14941 gatgcgggcg tcgatgtgct ggatattggt atgtccggca ccgaagaaat ctatttcgcc
    15001 acgttccatc tcggcgtgga tggcggcatt gaagttaccg ccagccataa cccgatggat
    15061 tacaacggca tgaagctggt gcgcgaaggg gctcgcccga tcagcggcga taccggactg
    15121 cgcgacgtcc agcgtctggc agaagccaac gacttccctc ccgtcgatga aaccaaacgt
    15181 ggtcgctatc agcaaatcaa cctgcgcgac gcatatgttg atcacctgtt cggttatatc
    15241 aatgtcaaaa acctcacgcc gctcaagctg gtaatcaact cagggaacgg tgcggcgggt
    15301 ccggtggtgg acgccatcga agcccgcttt aaagccctcg gcgcacccgt ggaattgatc
    15361 aaagtgcaca atacgccgga cggcaatttc cccaacggta ttcctaaccc gctgctacct
    15421 gaatgccgcg acgacactcg caatgcggtc attaaacacg gcgcggatat gggcattgct
    15481 tttgatggcg attttgaccg ctgtttcctg tttgacgaaa aagggcagtt tatcgagggc
    15541 tactacattg tcggtctgct ggcagaagcg ttcctcgaaa aaaatcccgg cgcgaagatc
    15601 atccacgatc cacgtctctc ctggaatacc gttgatgtgg tgactgccgc aggcggcacc
    15661 ccggtaatgt cgaaaaccgg acacgccttt attaaagaac ggatgcgcaa ggaagacgcc
    15721 atctacggtg gcgaaatgag cgcccaccat tacttccgtg attttgctta ctgcgacagc
    15781 ggcatgatcc cgtggctgct ggtagccgaa ctggtgtgcc tgaaaggaaa gacgctgggc
    15841 gaactggtgc gcgaccggat ggcagcgttt ccgtcaagcg gtgagatcaa cagcaaactg
    15901 gcgcaacccg ttgaggcgat taaccgcgtg gaacagcatt ttagccgtga ggcgctggcg
    15961 gtggatcgca ccgatggcat cagcatgacc tttgccgact ggcgctttaa tctgcgctcc
    16021 tccaacaccg aaccggtggt gcggttgaat gtggaatcac gcggtgatgt gccgctgatg
    16081 gaagcgcgaa cgcgaactct gctgacgttg ctgaacgagt aatgtcggat cttcccttac
    16141 cccattgcgg gtaaggggct aataacagga acaacgatga caaatctaaa aaagcgcgag
    16201 cgagcgaaaa ccaatgcatc gttaatctct atggtgcaac gcttttcaga tatcaccatc
    16261 atgtttgccg gactatggtt ggtttgcgaa gtgagcggac tgtcattcct ctacatgcac
    16321 ctgttggtgg cgctgattac gctggtggtg ttccagatgc tgggcggcat caccgatttt
    16381 tatcgctcat ggcgcggtgt tcgggcagcg acagaatttg ccctgttgct acaaaactgg
    16441 accttaagcg tgattttcag cgccggactg gtggcgttca acaatgattt cgacacgcaa
    16501 ctgaaaatct ggctggcgtg gtatgggctg accagcatcg gactgttggt ttgccgttcg
    16561 tgtattcgca ttggggcggg ctggctgcgt aatcatggct ataacaagcg catggtcgcg
    16621 gtggcggggg atttagccgc cgggcaaatg ctgatggaga gcttccgtaa ccagccgtgg
    16681 ttagggtttg aagtggtggg cgtttaccac gacccgaaac cgggcggcgt ttctaacgac
    16741 tgggcgggta atctgcaaca gctggtagag gatgcgaaag cgggcaagat tcataacgtc
    16801 tatatcgcaa tgcagatgtg cgacggcgcg agagtgaaaa aactggtcca tcaactggcg
    16861 gataccacct gttcggtgct gctgatcccc gatgttttta ccttcaatat tcttcattcc
    16921 cgtatcgaag agatgaacgg cgttccggtg gtgccgctgt acgatacgcc gctttccggg
    16981 gttaaccgcc tgctaaaacg tgcggaagac attgtgctgg cgacgcttat tctgctgctg
    17041 atctccccgg tgctgtgctg tattgcgcta gcggtgaaac tcagttcacc ggggccggtt
    17101 attttccgcc agactcgcta cggcatggat ggcaagccga tcaaagtgtg gaagttccgt
    17161 tccatgaaag tgatggagaa cgacaaagtg gtgactcagg cgacgcagaa cgatccgcgc
    17221 gtcaccaaag tggggaactt tctgcgccgc acctcgctgg atgaactgcc gcagtttatc
    17281 aatgtgctga ccggggggat gtcgattgtc ggtccacgtc cacacgcggt ggcgcataac
    17341 gaacagtatc gccagctcat tgaaggctac atgctgcgtc ataaagtgaa accgggcatt
    17401 actggctggg cgcagattaa tggctggcgc ggcgaaaccg acacgctgga gaaaatggaa
    17461 aaacgcatcg agttcgacct tgagtacatc cgcgaatgga gcgtctggtt cgatatcaaa
    17521 atcgttttcc tgacggtatt caaaggcttc gttaacaaag cggcatattg atatgagctt
    17581 acgtgaaaaa accatcagcg gcgcgaagtg gtcggcgata gccacggtga tcatcatcgg
    17641 cctcgggctg gtgcagatga ccgtgctggc gcggattatc gacaaccacc agttcggttt
    17701 gcttaccgtg tcgctggtga tcatcgcgct ggcagatacg ctttctgact tcggtatcgc
    17761 taactcgatt attcagcgaa aagaaatcag ccaccttgaa ctcaccacgc tgtactggct
    17821 gaacgtcggg ctggggcttg tggtgtgcgt ggcggtgttt ttgttgagtg atgccattgg
    17881 cgacgtgctg aataacccgg acctggcacc gttgattaaa acattgtcgc tggcgtttgt
    17941 ggtgatcccc cacgggcaac agttccgcgc gttgatgcag aaggagctgg agttcaacaa
    18001 aatcggcatg atcgaaacca gcgcggtgct ggcgggcttc acttttacag tggttagcgc
    18061 ccatttctgg ccgctggcga tgaccgcgat cctcggttat ctggtcaata gtgcggtaag
    18121 aacgctgctg tttggctact ttggtcgaaa aatttatcgc cccggtctgc atttctcgct
    18181 ggcgtctgtt gcaccgaact tacgctttgg tgcctggctg acggcggaca gcatcatcaa
    18241 ctatctcaac accaaccttt cgacgctggt gctggcgcgt attctcggcg cgggcgtggc
    18301 agggggatac aacctggcgt acaacgtggc cgttgtgcca ccgatgaagc tgaacccaat
    18361 catcacccgc gtgttgtttc cggcattcgc caaaattcag gacgacaccg aaaagctgcg
    18421 cgtcaacttc tacaagctgc tgtcggtggt ggggattatc aactttccgg cgctgctcgg
    18481 gctgatggtg gtgtcgaata attttgtacc gctggtcttt ggtgagaagt ggagcagcat
    18541 tattccggtg ctgcaattgc tgtgtgtggt ggggctactg cgctcagtgg gtaacccgat
    18601 tggttcgcta ctgatggcga aagcgcgggt cgatatcagc tttaaattca acgtattcaa
    18661 aacctttctg tttattccgg cgattgttat aggtgggcag atggcgggcg caatcggcgt
    18721 cacgctcggc ttcctgctgg tgcagattat caacaccatt ctgagttact tcgtgatgat
    18781 taaaccggtt cttggttcca gttatcgcca gtacatcctg agtttatggc tgccgtttta
    18841 tctctcgctg ccgacgctgg tggtcagtta tgtactgggc attgtgctga aagggcaact
    18901 ggcgctgggg atgctgctgg cggtgcaaat agccgcaggc gtgctggcgt ttgtggtgat
    18961 gattgtgctg tcgcgccatt cgttggtggt ggaagtgaag cgtcagtttt gtcgcagcga
    19021 aaaaatgaaa atgcttttac gggcggggtg aatggttgtt ttaggctccg acaacggttg
    19081 ccgggtgcag cgtaaaacgc cttatccatc ctacggttct gtgctcggga cctgtaggcc
    19141 tgataagacg cgtcagcgtc gcatcaggca ctgaatgccg gatgcggcgt gaacgcctta
    19201 tccggcctac ggttctgtgc tcgggtcttg taggcctgat aagacgcgtc agcgtcgcat
    19261 caggcactga atgccggatg cggcgtgaac gccttatccg gcctacggtt ctgtgctcgg
    19321 gtcttgtagg cctgataaga cgcgccagcg tcgcatcagg caaaaaaccg caacgaacac
    19381 aacaaacaca gcctcacctg gcatcaaatt tatgaggaaa aaatgaaatt acttattctg
    19441 ggcaaccaca cttgcggcaa tcgtggcgac agcgccatcc tgcgcgggtt acttgatgcc
    19501 atcaatatcc tcaatccaca tgccgaagtg gacgtgatga gccgctatcc ggtcagttct
    19561 tcctggctgc tcaaccgccc ggtaatgggc gatccgctgt tcctgcaaat gaaacaacac
    19621 aacagcgcgg cgggcgttgt cgggcgcgtt aaaaaagtcc tccgtcgccg ctatcaacac
    19681 caggtactgc tctcacgcgt caccgacact ggcaagctgc gtaatatcgc catcgcccag
    19741 ggattcaccg acttcgtgcg cctgctgtca ggttacgacg ccattatcca ggtcggcgga
    19801 tcgttttttg tcgatctcta cggcgtgccg cagtttgaac atgcgctttg cacgtttatg
    19861 gcgaaaaagc cgctgtttat gattggtcac agcgtcggtc ccttccagga tgagcaattt
    19921 aaccaactgg cgaactatgt ctttggtcat tgcgacgcgc tgatcctgcg cgaatcggtc
    19981 agccttgatc tgatgaaacg cagcaatatc actaccgcaa aagttgaaca tggcgtcgat
    20041 accgcgtggc tggtcgatca ccacacagaa gacttcaccg ccagctatgc cgtccaacac
    20101 tggctggacg ttgccgcaca acagaaaacg gtggcaatta ccctgcgcga actggcaccg
    20161 ttcgacaaac gtctcggcac cactcaacaa gcgtatgaaa aagcctttgc cggggtggtc
    20221 aatcgcattc tcgacgaagg ctatcaggtg attgcgctct ccacctgtac gggcatcgac
    20281 agctataaca aagacgaccg catggtggcg ctcaacctgc gccagcatat cagcgatcct
    20341 gcccgttacc acgtggtgat ggatgaactc aacgatctgg aaatgggcaa aattctcggt
    20401 gcctgtgaac tcaccgtcgg tacgcgcctg cactccgcca ttatctcgat gaactttgcc
    20461 actccggcaa ttgccatcaa ctacgaacat aaatccgccg ggattatgca gcagctggga
    20521 ttgccagaga tggcaattga tatccgtcat ttactggacg gcagcctgca agcgatggtt
    20581 gcggatacct taggccagct tccggcgctg aacgcacgac ttaacgaagc cgtcagtcgc
    20641 gaacgtcaga cgggaatgca gatggtgcag tccgtactgg aacgcattgg ggaggtaaaa
    20701 tgaaggtcgg cttcttttta ctgaaattcc cgctgtcgtc ggaaaccttc gtcctcaatc
    20761 aaattaccgc gtttattgat atgggatttg aggtggagat tgtcgcgctg caaaaaggcg
    20821 acacacaaaa cacccacgcg gcatggacga aatacaacct tgcagccaga acccgctggt
    20881 tacaggacga acctacgggc aaagtggcga agctgcgcca ccgcgccagc cagacgttac
    20941 gcggcattca tcgtaaaaat acctggcagg cgctcaacct caaacgctat ggcgcagagt
    21001 cgcggaacct gattttgtct gccatttgcg gccaggttgc aacaccgttt catgctgatg
    21061 tgttcatcgc tcattttggc cctgcggggg taaccgcagc aaaactacgc gaactgggtg
    21121 tcattcgcgg caaaattgcc accatcttcc acggtattga tatctccagt cgggaagtgc
    21181 tcaaccacta cactcccgaa tatcaacaac tgtttcgccg tggcgacctg atgttaccga
    21241 taagcgatct gtgggccgga aggctgcaaa aaatgggctg cccgagggaa aaaatcgccg
    21301 tatcgcgcat gggcgtggac atgacacgtt ttagcccgcg tccggtgaaa gcgcccgcaa
    21361 cgccgctgga gattatttcc gtcgcacgct taaccgagaa aaaaggtctg catgtggcga
    21421 ttgaagcctg ccgtcagttg aaagagcagg gcgtggcatt tcgctatcgc attctcggca
    21481 ttggcccgtg ggaacgacgc ctgcgcaccc tcatcgaaca atatcaactg gaagatgtgg
    21541 tggagatgcc gggctttaaa ccgagccacg aagtgaaagc gatgctcgac gacgcggatg
    21601 tcttcctgtt gccatcggta acgggtgcgg acggtgatat ggaaggcatt ccggtggcgc
    21661 tgatggaagc aatggcggtc ggcattccgg tggtttctac tctgcatagc ggaataccgg
    21721 aactggtgga ggctgacaaa tccggctggc tggtgcctga gaacgatgct cgcgcactgg
    21781 cgcaacgact ggcggcgttt agccaactgg acaccgacga attggctccg gtcgtgaaaa
    21841 gcgcgcgcga aaaagttgaa cacgatttta accagcaggt gattaatcga gaactcgcca
    21901 gcttgttaca ggctttatag aggagaacgt atgccattta aaacactctc ccgacgcacc
    21961 ttcctgacgg caagctcggc gcttgtcttc ctccataccc ctttcgctcg cgcacttccc
    22021 gcccgacaaa gcgttaacat taacgactac aacccacacg actggatcgc ctcatttaaa
    22081 caagccttca gcgaagggca aacagtcgtc gtgcctgctg gattcgtttg tgacaatatc
    22141 aacaccggca tcttcattcc tgcaggtaaa acgttacaca tccttggaag cctgcgcggc
    22201 aacggcagag ggcgatttgt cttacaggac ggcagccagg tgacagggga ggagggcggc
    22261 agtatgcata acatcaccct ggatgtgcgt ggttctgact gcaccatcaa agggctggcg
    22321 atgagcggct ttggcccggt aacgcagatt tatatcggcg gcaaaaacaa acgggtcatg
    22381 cgcaacctga ccatcgataa cctgaccatt agccacgcta attacgccat cttacggcag
    22441 ggatttcata accagattat cggtgccaac atcaccaact gtaaatttag tgatttacag
    22501 ggcgacgcca ttgaatggaa cgtggcgatt aacgaccgtg atattttgat atccgaccat
    22561 gtcatcgagc gcatcaactg taccaacggc aaaatcaact ggggaatcgg cataggcctt
    22621 gcgggaagta cctacgataa taagtacccg gaagaccagg cagtgaaaaa ctttgtcgtg
    22681 gcgaatatca cgggatcgga ttgtcggcag ttgatccatg ttgaaaatgg taaacatttt
    22741 gttattcgta atatcaaagc ccgcaatatc acgccggatt tcagtaagaa agcaggcatt
    22801 gataacgcga cagttgctat ttacggttgt gacaatttcg tgattgataa tattgaaatg
    22861 attaatagcg ccgggatgtt aatcggctat ggggtaatta aaggcaaata tctctcgata
    22921 ccgcaaaatt tccgagtgaa taatattcaa ctggataaca cccatcttgc ttataaattg
    22981 cgcggcatcc aaatctccgc tgggaatgcc gtctcctttg tggcgctgac taacattgag
    23041 atgaagcgtg cgtcgctgga gttacacaac aaaccgcaac acctttttat gcgaaatatc
    23101 aaggtgatgc aggaatcctc agttggacca gcattgagca tgaactttga catgcgcaaa
    23161 gacgttcgcg gcatttttat ggcgaaagaa gaaacactgc tgtcttttgc aaatgttcat
    23221 gcggtgaatg aaagagggca gagttccgtc gatatcgaca ggattaatca ccatattgtt
    23281 aatgtggaaa agattaactt tagattgccg gaacggaggg agtagatttg cgaccattca
    23341 taaacttact ggatataatg ttctaaaaga ttataatcct cacactaact gttaaagctg
    23401 gggctgggca gcattgacta ctcagggaag gtactcatct ttatcttgtg tttcttacgt
    23461 aacaaccaga ctaaagtgat ttatgaacga taacgttttg ctcataggag cttccggatt
    23521 cgtaggaacc cgactacttg aaactgcaat tgctgacttt aatatcaaga acctggacaa
    23581 acagcagagc cacttttatc cagaaatcac acagattggc gatgttcgcg atcaacaggc
    23641 actcgaccag gcgttagccg gttttgacac tgttgtacta ctggcagcgg aacaccgcga
    23701 tgacgtcagc cctacttctc tctattatga tgtcaacgtt cagggtaccc gcaatgtgct
    23761 ggcggccatg gaaaaaaatg gcgttaaaaa tatcatcttt accagttccg ttgctgttta
    23821 tggtttgaat aaacacaacc ctgacgaaaa ccatccacat gaccctttca accactacgg
    23881 caaaagtaag tggcaggcag aggaagtgct gcgtgaatgg tataacaaag caccaacaga
    23941 acgttcatta accatcatcc gtcctaccgt tatcttcggt gaacgcaacc gcggtaacgt
    24001 ctataacttg ctgaaacaga tcgctggcgg caagtttatg atggtgggcg cagggactaa
    24061 ctataagtcc atggcttatg ttggaaacat tgttgagttt atcaagtaca aactgaagaa
    24121 tgttgccgca ggttatgagg tttataacta cgttgataag ccagacctga acatgaacca
    24181 gttggttgct gaagttgaac aaagcctgaa caaaaagatc ccttctatgc acttgcctta
    24241 cccactagga atgctgggtg gatattgctt tgatatcctg agcaaaatta cgggcaaaaa
    24301 atacgctgtc agctctgtgc gcgtgaaaaa attctgcgca acaacacagt ttgacgcaac
    24361 gaaagtgcat tcttcaggtt ttgtggcacc gtatacgctg tcacaaggtc tggatcgaac
    24421 tctgcagtat gaattcgtcc atgccaaaaa agacgacata acgtttgttt ctgaataatg
    24481 caggcgagtc agagaaaaaa ggaccaggtt ggactacgat tattcggaaa gaggtactga
    24541 ttaaattatc agtatgtgga tttccgagta atccgagtat cccagatgct gatggtgtgt
    24601 agtgttatcg taaggtttaa tcaaagttat gcggaactaa gctttggtat atagccataa
    24661 cgtttggcta accgatggca ataatttacg cactatcagc cacttagagg taggaaaata
    24721 atgacgaatt taaaagcagt tataccggta gcgggtcttg ggatgcatat gttgcctgcc
    24781 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt
    24841 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag
    24901 aacgcagtcg aaaaccactt cgacacctct tatgaattag aatctctcct tgaactgcgc
    24961 gtgaagcgcc aactgctggc ggaagtacag tccatctgtc cgccgggcgt gaccattatg
    25021 aacgtgcgtc agggcgaacc tttaggttta ggccactcca ttttgtgtgc acgccccgcc
    25081 attggtgaca acccatttgt cgtggtactg ccagacgtag tgatcgacga cgccagcgcc
    25141 gacccgctgc gctacaacct tgcggccatg attgcgcgct tcaacgaaac gggccgtagc
    25201 caggtgctgg caaaacgtat gccgggtgac ctctctgaat actccgtcat tcagaccaaa
    25261 gaaccgctgg accgtgaagg taaagtcagc cgcattgttg aattcatcga aaagccggat
    25321 cagccgcaga cgctggactc agacgtcatg gccgttggtc gctatgtgct ttctgccgat
    25381 atttggccgg aacttgaacg tactcagcct ggtgcgtggg gacgtattca gctgacagat
    25441 gcaattgctg agctggcgaa aaaacagtcc gttgatgcaa tgctgatgac cggcgacagt
    25501 tacgactgcg gtaaaaaaat gggctatatg caggcgtttg tgaagtatgg actacgcaac
    25561 ctcaaagaag gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa
    25621 tctgaccgaa tgtaacggtt gataagaaaa ttataacggc ggtgaagatt cgtgacgaaa
    25681 gtaatttgga gcgaatcttc gggacgttat ttgtaaattt tcataataac aaagagttat
    25741 caatagtatt ttagtcaaag ttttcaggat tttccttgtt tccagagcgg attggtaaga
    25801 caattagctt ttgaattttt cgggtttggc gcgggtggac aacgctcgtc acatcgtaga
    25861 catgcatgca gtgctctggt agctgtaaag ccaggggcgg tagcatgcat taatacctct
    25921 atcaatcaaa ctgagagccg cttatttcac agcatgctct gaagtaatat ggaataaatt
    25981 aagtgaaaat acttgttact ggtggcgcag gatttattgg ttctgctgta gttcgtcaca
    26041 ttataaataa tacgcaggat agtgttgtta atgtcgataa attaacgtac gccggaaacc
    26101 tggaatcact tgctgatgtg tctgattctg aacgctatgt ttttgaacat gcggatattt
    26161 gcgatgcagc tgcaatggca ctgatttttg ctcagcatca gccagatgca gtgatgcacc
    26221 tggcggctga aagccatgtg gatcgttcaa ttgcaggccc tgctgcattt attgaaacca
    26281 atattgttgg tacttatgtc cttttggaag cagctcgcaa ttactggtct gctcttgatg
    26341 gcgacaagaa aaatagcttc cgttttcatc atatttctac tgacgaagtt tatggcgatc
    26401 tgcctcatcc tgacgaagta aataataaag aagaattacc cctctttact gagacgacag
    26461 cttacgcgcc aagcagccca tattctgctt ctaaagcatc cagcgatcat ttagtccgcg
    26521 cgtggaaacg tacctatggt ttaccgacca ttgtgactaa ctgttcgaat aactacggcc
    26581 cttatcactt tccggaaaaa ttgattccgc tggtaattct taatgctctg gaaggtaagg
    26641 cattacctat ttatggcaaa ggggatcaaa ttcgcgattg gctgtatgtt gaagatcatg
    26701 cgcgtgcgtt atataccgtt gtaaccgaag gtaaagcggg tgaaacttat aacattggcg
    26761 gacacaacga aaagaaaaac atcgatgttg tgctgactat ttgtgatttg ttggatgaga
    26821 ttgtaccgaa agagcaatct tatcgtgagc aaattactta tgttgccgat cgtccgggac
    26881 acgatcgccg ttatgcgatt gatgcagaga agattagccg cgaattgggc tggaaaccgc
    26941 aggaaacgtt tgagagcggg attcgtaaaa cggtggagtg gtatttggct aatgcaaaat
    27001 gggttgataa tgtgaaaagt ggtgcctatc aatcgtggat tgaacagaac tatgagggcc
    27061 gccagtaatg aatatcctcc ttttcggcaa aacagggcag gttggttggg aactacagcg
    27121 tgcgctggca cctttgggta atttgattgc tcttgatgtt cactctactg attattgtgg
    27181 tgattttagt aatcctgaag gtgtagctga aaccgtaaga agcattcggc ctgatattat
    27241 tgtcaacgca gcagctcaca ccgcagtaga caaagcagaa tcagaaccgg agtttgcaca
    27301 attacttaac gcaacaagtg tcgaagcgat tgcgaaagca gcaaatgaag ttggagcttg
    27361 ggttatccat tactcgactg attacgtctt ccctggaaat ggcgacacgc catggctgga
    27421 gacggatgca accgcaccgc taaatgttta cggtgaaacc aagttagccg gagaaaaagc
    27481 gttacaggaa cattgcgcga aacatcttat tttccgtacc agctgggtat atgcaggtaa
    27541 aggaaacaat tttgccaaga caatgttacg tctggcaaaa gagcgtgaag aattagaggt
    27601 tattaacgat cagtttggtg cgccaacagg tgctgaactg ctggctgatt gtacagcaca
    27661 tgccattcgt gtcgcactga ataaaccgga tgtcgcaggc ttgtaccatt tggtagccag
    27721 tggtaccaca acctggtacg attatgctgc gctggttttt gaagaggcgt gcaaagtagg
    27781 cattcccctt gcactcaaca agctcaacgc agtaccaaca tcagcctatc atacaccagc
    27841 ttgtcgtcca cataactctc gccttaatac agaaaaattt cagcagaact ttgcgcttgt
    27901 cttgcctgac tggcaggttg gcgtaaaacg aatgctcaac gaattgttta cgactacagc
    27961 aatttaatag tttttgcatc ttgttcgtga agataaagca agatgaatta aaaggaatga
    28021 taaaatgaaa acgcgtaaag gtattatttt agcgggtggt tctggtactc gtctttatcc
    28081 tgtgactatg gctgtcagta aacagctgtt accgatttat gataaaccga tgatctatta
    28141 cccgctttct acactgatgt tagcgggtat tcgcgatatt ttgattatca gtacgccaca
    28201 ggatactcct cgttttcaac agctgctggg tgacgggagc cagtgggggc taaatcttca
    28261 gtacaaagtg caaccgagtc cagatggtct tgcgcaggca tttatcatcg gtgaagagtt
    28321 tatcagtggt gatgattgtg ctttggttct cggtgataat atcttttacg gtcacgatct
    28381 gccgaagtta atggatgtcg ctgttaacaa agaaagtggc gcaacggtat ttgcctatca
    28441 cgttaatgat cctgaacgct acggtgtcgt tgagtttgat aaaaacggta cggcaataag
    28501 cctggaagaa aaaccgttac aaccaaaaag taattatgcg gtaactgggc tttatttcta
    28561 tgataatgac gttgtcgaaa tggcgaaaaa ccttaagcct tctgcccgtg gcgaactgga
    28621 aattaccgat attaaccgta tatatatgga gcaggggcgt ttatctgttg ccatgatggg
    28681 acgtggttat gcatggctgg acacggggac acatcaaagt cttattgaag caagtaactt
    28741 cattgcaaca attgaagagc gtcaggggct gaaagtttcc tgtccggaag aaattgctta
    28801 ccgtaaaggc tttattaatg ctgaacaagt gcaagtgtta gctgaatcgc taaagaaaaa
    28861 tgcttatggg cagtatttat taaagatggt tgaaggttat taataaaatg aaagtcataa
    28921 aaactagtat tccggatgtt ttaatttttg aaccaaaagt atatggagat gaacgtggct
    28981 tttttatgga aagctttaat caaaaagttt tcgatgaggc tgtagggcgt aaaattgaat
    29041 ttgtgcagga taaccactct caatctagta aaggcgtgtt gcgagggtta cattatcaat
    29101 tagaacctta tgctcaagga aagcttgttc gctgtgttga aggtgaagta tttgacgtcg
    29161 cagttgacat acgtaaatca tcacccactt ttggacagtg ggtaggtgta tatttaaatg
    29221 ctaaaaacaa gcgacaatta tggataccgg aaggatttgc tcatggattt attgtaacta
    29281 gcgaaaaagc cacattcctt tataaagcaa ctaattacta tatgccctct catgaaggtg
    29341 ggataatatg gaacgataaa tcattgaata tacaatggcc tttaatgaga gaaatgatta
    29401 tatcagcaaa agatcaacag caaatatcgc tgactgaata tttagaaatt gaatcatcaa
    29461 aaaaatgatg agttggtata aacccaaacc aaaactaact aatgattaga gatttcatgt
    29521 taagattctt ttctaacaaa gtaaaagatc atgatttttt gaaggtgttt accaatgcgt
    29581 c
//
LOCUS       DAZFFG010000053        29097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFFG010000053 DAZFFG010000000
VERSION     DAZFFG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.053"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..2657
                     /gene="cas3"
                     /locus_tag="ACHJ7J_002609"
     CDS             <1..2657
                     /gene="cas3"
                     /locus_tag="ACHJ7J_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO5000827.1"
                     /translation="TFFDFWGKTRRGEKEGGDDYHLLCWHSLDVAAMGYLMVKRNCFG
                     LADYFYQLGISDKEQAAQFFAWSLCWHDIGKFAHSFQQLYLPPELKIQEGARKNYEKI
                     SHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDN
                     FLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKQLSWYISATVVLADWTGSS
                     TRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFTGINTLFPFIENPTPLQQ
                     KVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAAGKAQGLFFGLPTMATANTM
                     YDRLVKTWLSFYSPESRPSLVLAHSARTLMDRFNESLWSGDLIGSEEPDEQAFSQGCA
                     AWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMS
                     CILEGLIERQARGGNSVILLSATLSQQQRDKLVTAFARGTEGQQEAPLLEKDDYPWLT
                     HVTQTDVNSHRVATRKDVERSVSVGWLHSEQECIARIESSVSQGKCIAWIRNSVDDAI
                     KVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQV
                     LEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAP
                     VWDDSPGDEWFGRAMRNSAFVYPDHGRIWLTQRVLRGQGAIQMPHAARLLIESVYGED
                     VVMPEGFARSEQEQVGKYYCDRTMAKKFVLNFRPGYAASINDYLPEKLSTRLAEESVS
                     LWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCIEQRQE
                     PEMANVILVTDDESCGYSAMEGLTGKVG"
     gene            2754..4316
                     /gene="casA"
                     /locus_tag="ACHJ7J_002610"
                     /gene_synonym="cse1"
     CDS             2754..4316
                     /gene="casA"
                     /locus_tag="ACHJ7J_002610"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO5000828.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPDVPGAQTTKFNKDHFIKRGVTEYLCPHCLALALFSLQLNA
                     PAGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQDDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHRFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFSRQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4313..4852
                     /gene="casB"
                     /locus_tag="ACHJ7J_002611"
                     /gene_synonym="cse2"
     CDS             4313..4852
                     /gene="casB"
                     /locus_tag="ACHJ7J_002611"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO5000829.1"
                     /translation="MSIVKDGHKATLRKWHEELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVAANVKAIDERQPFAAQLAAVMSEGRFTCL
                     SAVKTPDELLRQLRRAVKLLNGSVNLISLADDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            4864..5919
                     /gene="cas7e"
                     /locus_tag="ACHJ7J_002612"
     CDS             4864..5919
                     /gene="cas7e"
                     /locus_tag="ACHJ7J_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO5000830.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5930..6676
                     /gene="cas5e"
                     /locus_tag="ACHJ7J_002613"
     CDS             5930..6676
                     /gene="cas5e"
                     /locus_tag="ACHJ7J_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO5000831.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQGGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDHFHDLKVSLPKLQDECWWEGEHDGLVVSKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6658..7308
                     /gene="cas6e"
                     /locus_tag="ACHJ7J_002614"
     CDS             6658..7308
                     /gene="cas6e"
                     /locus_tag="ACHJ7J_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO5000832.1"
                     /translation="MYLSRITLHTAQLLPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGRDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            7305..8228
                     /gene="cas1e"
                     /locus_tag="ACHJ7J_002615"
     CDS             7305..8228
                     /gene="cas1e"
                     /locus_tag="ACHJ7J_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO5000833.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGYIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARHPAESDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            8225..8518
                     /gene="cas2e"
                     /locus_tag="ACHJ7J_002616"
     CDS             8225..8518
                     /gene="cas2e"
                     /locus_tag="ACHJ7J_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO5000834.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWVTNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   8615..9192
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8615..8642
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaacc"
     gene            complement(9274..10311)
                     /gene="iap"
                     /locus_tag="ACHJ7J_002617"
     CDS             complement(9274..10311)
                     /gene="iap"
                     /locus_tag="ACHJ7J_002617"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO5000835.1"
                     /translation="MFSALRHCTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10563..11471
                     /gene="cysD"
                     /locus_tag="ACHJ7J_002618"
     CDS             10563..11471
                     /gene="cysD"
                     /locus_tag="ACHJ7J_002618"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO5000836.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            11473..12900
                     /gene="cysN"
                     /locus_tag="ACHJ7J_002619"
     CDS             11473..12900
                     /gene="cysN"
                     /locus_tag="ACHJ7J_002619"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO5000837.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVQEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            12900..13505
                     /gene="cysC"
                     /locus_tag="ACHJ7J_002620"
     CDS             12900..13505
                     /gene="cysC"
                     /locus_tag="ACHJ7J_002620"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO5000838.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13555..13878
                     /gene="ygbE"
                     /locus_tag="ACHJ7J_002621"
     CDS             13555..13878
                     /gene="ygbE"
                     /locus_tag="ACHJ7J_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO5000839.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            14072..14383
                     /gene="ftsB"
                     /locus_tag="ACHJ7J_002622"
     CDS             14072..14383
                     /gene="ftsB"
                     /locus_tag="ACHJ7J_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO5000840.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            14402..15112
                     /gene="ispD"
                     /locus_tag="ACHJ7J_002623"
     CDS             14402..15112
                     /gene="ispD"
                     /locus_tag="ACHJ7J_002623"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO5000841.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            15112..15591
                     /gene="ispF"
                     /locus_tag="ACHJ7J_002624"
     CDS             15112..15591
                     /gene="ispF"
                     /locus_tag="ACHJ7J_002624"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO5000842.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15588..16637
                     /gene="truD"
                     /locus_tag="ACHJ7J_002625"
     CDS             15588..16637
                     /gene="truD"
                     /locus_tag="ACHJ7J_002625"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO5000843.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16618..17379
                     /gene="surE"
                     /locus_tag="ACHJ7J_002626"
     CDS             16618..17379
                     /gene="surE"
                     /locus_tag="ACHJ7J_002626"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO5000844.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            17373..17999
                     /gene="pcm"
                     /locus_tag="ACHJ7J_002627"
     CDS             17373..17999
                     /gene="pcm"
                     /locus_tag="ACHJ7J_002627"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO5000845.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            18190..19278
                     /gene="nlpD"
                     /locus_tag="ACHJ7J_002628"
     CDS             18190..19278
                     /gene="nlpD"
                     /locus_tag="ACHJ7J_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO5000846.1"
                     /translation="MVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGT
                     TSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYS
                     GSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGN
                     AITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAP
                     VTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAII
                     ATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGS
                     TGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            19341..20333
                     /gene="rpoS"
                     /locus_tag="ACHJ7J_002629"
     CDS             19341..20333
                     /gene="rpoS"
                     /locus_tag="ACHJ7J_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO5000847.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(20453..20860)
                     /locus_tag="ACHJ7J_002630"
     CDS             complement(20453..20860)
                     /locus_tag="ACHJ7J_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5000848.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLRRLSAEEQELFMQLVRKMMST"
     gene            21007..21600
                     /locus_tag="ACHJ7J_002631"
     CDS             21007..21600
                     /locus_tag="ACHJ7J_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO5000849.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALAAFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            21600..23027
                     /locus_tag="ACHJ7J_002632"
     CDS             21600..23027
                     /locus_tag="ACHJ7J_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO5000850.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESSYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            23038..23274
                     /locus_tag="ACHJ7J_002633"
     CDS             23038..23274
                     /locus_tag="ACHJ7J_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO5000851.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(23315..23971)
                     /gene="pphB"
                     /locus_tag="ACHJ7J_002634"
     CDS             complement(23315..23971)
                     /gene="pphB"
                     /locus_tag="ACHJ7J_002634"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5000852.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWRASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(24077..26644)
                     /gene="mutS"
                     /locus_tag="ACHJ7J_002635"
     CDS             complement(24077..26644)
                     /gene="mutS"
                     /locus_tag="ACHJ7J_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO5000853.1"
                     /translation="MNESIDKDLSDHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYD
                     DAKRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPA
                     TSKGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLS
                     EPADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQ
                     FGTRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATR
                     RNLEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQ
                     DFTAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPV
                     QALREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLE
                     RLEVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPEL
                     KEYEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYT
                     LNYTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKST
                     YMRQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANI
                     LHNATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEK
                     MEGVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLREL
                     ESISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            26804..28264
                     /locus_tag="ACHJ7J_002636"
     CDS             26804..28264
                     /locus_tag="ACHJ7J_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5000854.1"
                     /translation="MKTTLTQSFIINKLSVHVKPELNSSGKVVFEPNPQQKPYIVFDD
                     HRDSPVGFGVKVSLTKKTYVIQRRVSSGTRDVKEGKKPSSVLKVKVGNVSDFPSIDQA
                     REAARQLVQTMIATKRNPNRIKRETEASELTISEVFAQYRQHLLGRSKPAKPNTLSVL
                     DKAENRLKEWQALRVKDLTGNDILRKFDEIASRARTAAEQTFRWANVAVKHAIEIEAG
                     NAQTQQRQPSLSYNPFSILKVQKKFRTRSELEDSYKAKGVRNPLSPKDSLGRFLTALH
                     NKRSFNRLGCDYLLLTVLLGARKEETASLCWREYLTNEEAKTTSYVDLENRKIRFYDT
                     KNRNDHELPICDAVKRVLEDRRDIVQEAETRQERLKWVFPARSKRSKAGHYSDSKSLR
                     EYLCTEAEIVKLGMHDLRRTFGRLAEELTSYAVVKKLLNHRNTTDPTERYAVPDEDRV
                     YEALQRIELHMLMTAPTLYNALLASAKYPPLSDNKE"
     gene            28268..28867
                     /locus_tag="ACHJ7J_002637"
     CDS             28268..28867
                     /locus_tag="ACHJ7J_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000855.1"
                     /translation="MSVVETLRELSEVWQLFGDMPDDTTLGVDLAALYLGISVKTLAR
                     YRQNGDGPNYIQYQSEDSKARNQRVNYLLKDLRSWRDSHRVSSTMHAAQVRGLAFTCL
                     SDFTEPQPFWRITNNTGSTSKIISHALTIPDESFKQILLEPNAEVIWISIEEALFVSW
                     INKDERSIWHKMFILTLNNIVSASAAEQERYILKDIFDD"
BASE COUNT         7135 a         7164 c         7799 g         6999 t
ORIGIN      
        1 tgactttctt tgatttctgg ggaaaaaccc gacgcggcga gaaagaaggg ggcgacgact
       61 atcaccttct ttgctggcat tcgctggatg tggccgcgat gggctattta atggttaaaa
      121 gaaattgctt cgggctggct gattattttt atcaattagg gatttctgac aaggaacagg
      181 cggctcaatt tttcgcctgg tcgctgtgct ggcacgatat tggaaaattt gcccactctt
      241 ttcaacaact ttacctgccc cctgaactca agattcagga aggtgccaga aaaaattacg
      301 aaaaaatctc tcattcaacg ctgggctact ggttgtggaa tcattattta agtgaatgtc
      361 aggagttact tccttcatct tcgctctctc cgcgcaaact taggcgagtg atagaaatgt
      421 ggatgcccgt gacgacaggg catcatggtc ggccccctga tcgtatggat gaattggata
      481 attttctgcc tgaagacaaa gctgcagcac gagattttct ccttgcaatc aaggcactgt
      541 ttccgcgaat agagatcccc gcattctggg atgatgacga gggcattgaa cttataaaac
      601 aactttcctg gtatatctct gcaaccgtcg tactcgcaga ctggacaggt tcgtcaacgc
      661 gattttttcc acgcgtcgca cacccaatgg atattaaagg ttactggcag aaaactttag
      721 ttcaggctca aaacgcctta accgtctttc ctctaaaagc aaaagtcgcg cctttcaccg
      781 gaattaatac gctgtttcct tttattgaga atccgacacc gttacagcaa aaggtgctgg
      841 atctggatat cagccagcct gggccacagt tatttattct ggaagatgtg actggtgcag
      901 gtaaaacaga agcggcgctt attcttgcgc acaggttgat ggctgcgggt aaagctcagg
      961 gtttgttttt tggcctgcca acaatggcaa cggccaatac catgtatgat cggctcgtca
     1021 aaacctggct ttctttctat tcgccagaat ctcgccccag tctggtgctg gcacacagtg
     1081 cccgcacatt aatggaccgc tttaatgaat cactctggtc cggtgattta atcgggtcag
     1141 aagaaccgga tgaacaagct ttcagccagg gttgtgcggc ctggtttgcc gacagtaaca
     1201 agaaggcgtt gctggctgaa attggcgtcg gcaccctgga tcaggcgatg atggcggtga
     1261 tgccgtttaa acataataat ctgcggcttc tggggctgag taacaaaatc ctgctggccg
     1321 atgagattca cgcctgtgac gcttacatgt cttgcattct tgaagggctg atcgagcgac
     1381 aggcgcgtgg tggaaacagc gttattttgc tttctgccac gttatctcaa cagcagcgcg
     1441 acaaacttgt taccgccttt gcgcgtggca cagagggcca gcaagaagct ccattgctgg
     1501 aaaaagatga ttacccctgg ctgacgcatg tcacgcaaac ggatgtgaat tcgcaccggg
     1561 tagcgacgcg caaagacgtt gagcgtagcg tgagcgtggg ttggctccat agcgaacaag
     1621 aatgtattgc gcgtatcgaa tcttcggtaa gtcaggggaa atgcatcgcc tggatccgga
     1681 attctgtcga tgacgctatt aaagttcatc gtcagttact tgcccgcggc gtcattcccg
     1741 cttccagcct ttcactcttt catagccgct ttgcttttag cgatcgccag cgaattgaaa
     1801 tggagacgct ggcacgcttt ggtaaagaag acggttcaca gcgtgccgga aaagtcctca
     1861 tttgtactca ggtcttagag cagagcgttg attgtgacct ggacgaaatg atctccgacc
     1921 tggcccctgt tgatttgctg attcagcgag cggggcgatt acagcggcat attcgcgata
     1981 ttaatggtca gttaaagcgt gacggaaaag acgagcgttc ccctcctgaa ttgctgattc
     2041 tggcacccgt ctgggacgac tctccgggtg atgaatggtt tggcagagcc atgcggaaca
     2101 gtgcatttgt ctaccccgat catgggcgaa tctggctgac gcagcgtgtg ctgcgtgggc
     2161 aaggcgctat tcagatgcca cacgcagccc ggcttcttat tgaatcagtt tacggtgagg
     2221 acgtggtaat gccggaagga tttgcccgca gcgagcagga gcaagtgggc aaatattact
     2281 gcgatcgcac aatggctaaa aagtttgtgc tgaacttcag gcccggctat gccgccagta
     2341 tcaacgatta ccttccggaa aaactgtcga cacgtctggc tgaagagtct gtttccctgt
     2401 ggctggctac ctgtattgac ggtgtggtga agccttatgc taccggtgct cacgcatggg
     2461 aaatgagcgt tgtcagagtg cgtcgaagct ggtggaaaaa acatcgggat gagttttctt
     2521 tactggaagg ggatgcgttc aggcagtggt gcattgaaca gcggcaagag ccggagatgg
     2581 caaacgtgat tttagtcacc gatgacgaaa gttgcgggta ttcggctatg gaaggattga
     2641 ctggcaaggt tggttgagat gcctgcgaca gggctctctg aatagttgat acaccgaaaa
     2701 ggatggaagg atggacaacc gaccagggat ggtcttttta cgagaggatg cgtatgaact
     2761 cgttttcact tctgacaacc ccgtggttgc ccgttcgtta taaagacgga acgacaggca
     2821 agctggcacc agtcgatctg gctgatgaaa atgttgtcga tatcgccgcg ccgcgagcag
     2881 atttacaagg tgcggcatgg cagtttttgc tggggctact acaaaccagc tttgcgccaa
     2941 aagatcatcg tcgttgggat gatatatggg aagacggact ggaagctgaa aagctaaggg
     3001 aagcattgct gtcattagaa catgctttcc agtttggccc agattcccct tcatttatgc
     3061 aggattttga ggcgctcacg ggagataaag ttcaggtcgc ttcgctactg cctgatgttc
     3121 ccggcgctca aacgacgaag tttaataaag accattttat caagcgtggt gtaacggaat
     3181 atctgtgccc ccattgttta gcattggcct tgttctcttt acagttgaat gcaccagcgg
     3241 gtggcaaagg ctaccgaacc ggtttacgcg gcggtgggcc gatgacgact ctgattgaat
     3301 tgcaggagta tcagggcaat caacaagccc ccttgtggcg caaactgtgg ctcaacgtga
     3361 tgccgcagga tgaagccgac ttaccgctac ccaaaaaatt tgacgatctg gttttcccct
     3421 ggcttggccc gacgcgtacc agcgaactgg ccggtgcggt ggtaaccgat gatcaggtca
     3481 ataaactcca ggcgtactgg ggaatgccgc ggcgtattcg tattgatttt aataccacga
     3541 cagtcggcaa ctgcgatatt tgcggtgagc aggacgacgc gcttctgagc ctgatgacga
     3601 ccaaaaatta cggtgcgaat tatgccatgt ggcagcatcc attaacgcct taccgtgtac
     3661 cacttaaaga gggtggcgag ttttactcag ttaaaccaca accaggcggt ttaatttggc
     3721 gcgactggtt aggccttatc gaaacgggta agtcagaaaa caatacggaa cttcccgctc
     3781 tggtggtgaa actctttaat gccagcagtc tcaagcaggc aaaagtaggc ctatggggat
     3841 ttggctatga tttcgacaac atgaaagccc gctgttggta cgaacaccgt ttcccgctgc
     3901 tgctcaataa aaaagaaggc cagataccga aactgcggct ggctgcgcaa acggcttcac
     3961 gcattctaag tctgttacgg agtgcattga aagaagcatg gttctccgat ccaaaaggtg
     4021 cacggggtga tttcagtttt gtggatatcg acttctggaa caaaactcag catcgcttcc
     4081 tgaggttagt gcgccaaatt gaagaaggtc aggatgcgga tgaattactc agcaaatgga
     4141 ataaggagat ttggttattc tcgcgtcagg attttgacga gcgtgtattc accaatcctt
     4201 atgagcccgt tgatttaaaa cgcgtcatga ccgcgcgcaa aaaatatttt acaacatcgg
     4261 cggagaagca aagtgctaaa gccgccaggg agaaaaagca ggaggctgct gaatgagtat
     4321 tgtaaaagac gggcacaaag ccacgctgcg taaatggcac gaagagttgc aggctaaacg
     4381 cggcaatcgt gccagtctgc ggcgtagcac aacggttaat gatgtttgct tgtcagaagg
     4441 gtttcgttct ctgctgatgc agacccacac tttgtggaaa attgaagcac aagagtggcg
     4501 ttttaccgca ctggcactgg tcgccgccgt tgccgccaat gttaaagcca ttgatgaacg
     4561 gcagcctttt gccgcacagc tggcggcggt aatgtcagag gggcgtttta cttgcctgtc
     4621 ggcagtaaaa acgccggatg aattactgcg ccagttacga cgcgcagtca aattattaaa
     4681 cggttccgtc aatcttatct ctttggcaga cgatattttc cgctggtgtc aggagagcga
     4741 cgacctgctc aatcatcacc gccgccagca gcggccaacc gaatttatcc gtatccgctg
     4801 ggcgctggaa tattaccagg ctggcgacgc cgataacgaa caaaatcaat aaaggactat
     4861 ctcatgacaa catttattca gcttcatttg ttaaccgctt accctgctgc caaccttaac
     4921 cgtgatgata ctggtgcgcc aaaaactgtg gtactgggtg gtgcaacgcg tctgcgcgtt
     4981 tcctcacaaa gtctgaaacg tgcgtggcgc acttctgcac tttttgaaca ggcactggcg
     5041 ggccatattg gtattcgcag tgggcgaatt gcgcgtgagg cggcaactat cctgattgag
     5101 aaaggaatcg aagagaaaaa agccatcgaa tgggcggcaa aaattgcaga ttatcttggg
     5161 aaagctaaaa acgacaaaaa accaaaagat ccgctcacta acgccgaaac tgaacaatta
     5221 gttcatatca gcccggcaga atttgacgcc gtaaaagcgc tggcccatca actggcagaa
     5281 gaaaagcgcg cgccaaaaga ggaagatctc gcgttgttac gtaaagaccg tatagcggta
     5341 gatatcgcta tgtttggtcg tatgctggcg aataaacctg agtttaatgt tgaagccgcc
     5401 tgccaggtcg cgcatgcgtt tggtgtcagt gaaacgattg tcgaagatga ttttttcacc
     5461 gccgttgatg atttgcgcca ggcctctgaa gatgccggtg ccgggcatct cggagaaacc
     5521 ggattcggtt cagcactttt ctacacctat atctgcattg ataaagatct gctggtagaa
     5581 aacctcggcg gtgacgaagc gttagctaat cagaccatac gcgcttttac ggaagccgcg
     5641 cttaaagttt ccccaaccgg taaacaaaat agctttgcca gccgtgccta cgcctcctgg
     5701 gcgctggcgg aaaaaggcac tgatcaaccg cgttctctgg cggcggcttt ctatgaaccc
     5761 attaatggta cccgtcagtt agatgtggcg gtgcagcgta ttacaacgct tcgcgaaaac
     5821 atgaatacgg tctacgaaca gaagactgaa tgcgcaagtt ttgacgtgat gaacaaacag
     5881 ggaagcatga aggacgtact ggactttatc tgcgcgtaag gggcggctta tgagccaata
     5941 tctgattttt cagcttcatg ggccaatggc atcctggggc gtcgatgccc ccggcgaagt
     6001 gcgtcatacc catgaactgc cttcgcgctc agcattgctg ggtctgctgg ctgccggggt
     6061 agggattcgg cgtgatgata ccgaacgctt aaacgcgttt aatcgccact attcactggt
     6121 ggtttgcgcc agccgtaacc cgcgctgggc acgggattat cacacgatcc agatgccaaa
     6181 agaggtgcgt aaagcgcgtt atttcagccg tcgcgaagag ttgagcgccc ctgatcttct
     6241 gagcgcgatt atctcccggc gcgactacta caccgatgcc tggtggatgg tggcggtggc
     6301 aacaacagcc gatgcgcctt acagcctcga acagttgcag ggcggtttac gccatccagt
     6361 ttttcctctt tatctggggc gaaaaagtca tccgctggca ttaccacttg cgccgttact
     6421 gctcgaaggc aacgcgtctg atgttttacg taacgcatac caacagtatc aggatcattt
     6481 ccacgacctg aaagtctcac tcccgaaact tcaggatgaa tgctggtggg aaggggagca
     6541 cgacggcctg gtcgtgagca aaatattacg ccgccgggat gttcctttaa atcgccagca
     6601 gtggctgttt ggggaacgca ccgtcaatca ggggccgtgg ctcagcaagg aggaaccatg
     6661 tacctctcaa gaataacctt acataccgct cagcttttgc ctgcgcagtt gctgcatctg
     6721 gtggatcgcg gtgaatatgt gatgcatcag tggctgtggg atctcttccc tagcggcaaa
     6781 gaaaggcaat ttctttatcg tcgggaggaa cttcagggtg cgtttcgctt ttttgtcctc
     6841 tcgcaggaac gcccggcgga aagtgaaact ttcactatcg aatgccggtc atttgctccg
     6901 gagctgcgta ccggacaatc cctttgtttc aacctgcggg cgaatcccac aatttgtaaa
     6961 gcgggcaagc gccacgatct gctgatggag gcgaaacggc aggtgagggg acaggcggaa
     7021 gggcgtgatg tctggttgca tcaacaacag gcggcgctgg actggctggc agcgcagggg
     7081 gagagaagcg gttttacact gctggatacc tcggttgatg cctaccgaca acagcaactg
     7141 cggcgggaaa actcccggca actgatccag ttcagtagcg tggattatac ggggatgctt
     7201 acggtcaccg atcccgggtt atttctgcaa cgtctcagtc aggggtatgg caaaagccgc
     7261 gcgttcggct gtggtctgat gctgatcaaa cccggagcag aggtgtgacg tttgtaccac
     7321 tgagtccgat cccgttaaaa gatcgcacct ctatgatctt cctccagtac ggtcaaatcg
     7381 acgtactgga cggcgctttc gtgctgatcg acaaaaccgg gatccgcacg cacattccgg
     7441 tgggatcggt cgcctgcatt atgctcgaac cgggaacgag agtttcccac gcggcggtgc
     7501 atctggccgc cacggtggga acactgctgg tctgggtcgg tgaagcgggc gttcgcgttt
     7561 actcttccgg acaacccgga ggggcgcggg cagataaatt actctaccag gcaaagctgg
     7621 ctttaacgga agatctacgc ctgaaggtgg tgcgcaaaat gtatgaatta cgttttcgtg
     7681 agccaccgcc agctcgccgt tcagtggatc agctacgggg aattgaggga tcccgcgttc
     7741 gccagaccta tgcattactg gcgaaacaat atggtgtgaa atggaatggt cgcaaatacg
     7801 atcctaaaga ctgggaaaaa ggcgatgttg tgaatcgctg catcagtgct gccacatcat
     7861 gtctgtacgg tatttctgaa gcggcagtat tagccgcggg atatgcgccc gctattggat
     7921 atattcatag tggcaaaccg ctttcatttg tttatgacat agccgatatc attaaatttg
     7981 attcggttgt gccaaaggca tttgaaatag cggcaagaca cccggcagaa tctgataaag
     8041 aagtcagatt agcctgtcgc gatattttcc gtagcactaa gttaacgggc aaattaatac
     8101 cgttaattga ggaagtcctt gctgcaggtg aaattgaacc accacaaccc gcgccggata
     8161 tgttaccgcc tgccatccct gaacctgaaa cgctgggtga tagtggtcac cgggggcgcg
     8221 gcggatgagc atggtcgtgg ttgttacaga aaatgtccct ccgcgcttac gtggacggct
     8281 cgcaatctgg ctactggaag tgcgtgccgg tgtgtatgtt ggtgatacat caaaacgtat
     8341 tcgggagatg atctggcagc aaattaccca actggctggt tgcggaaatg tggtgatggc
     8401 ctgggtgacc aataccgagt cgggttttga atttcagacc tggggagaaa acagacgtat
     8461 tccggtggat ttggatgggt tacgtttggt ttcttttctt cctgttgata atcaataggt
     8521 tatgtgttct ttaaaaataa ggaaatgttt gaatttagtt ggtagattgt tgatgtggaa
     8581 taaatttgtt taaaaacaga tatgtatgct tagtgtgttc cccgcgccag cggggataaa
     8641 ccggggctac ggccgagaca cccgtgctga ggttggtgtt ccccgcgcca gcggggataa
     8701 accgcccgaa accaaggcac tgtattctgc aatcgcgtgt tccccgcgcc agcggggata
     8761 aaccgagcgt gtttattgcg ttttaccggg gcaacaggtg ttccccgcgc cagcggggat
     8821 aaaccgattt atcgaatacc accacggcac cggcaaaagt gttccccgcg ccagcgggga
     8881 taaaccgcgg cactgctgcc gaatttttat cgcggtaacg tgttccccgc gccagcgggg
     8941 ataaaccggg ccttttcagc agattaaccg ccgcgattgc gtgttccccg cgccagcggg
     9001 gataaaccgc tcttttctgc ctcatacgac gcacgggaaa cgtgttcccc gcgccagcgg
     9061 ggataaaccg aataacacta attttttaca cggtattcgc acgtgttccc cgcgccagcg
     9121 gggataaacc tgcgtatatc tgttgatgcg cacggcgtgg cgggtgttcc ccgcgccagc
     9181 ggggataaac cgggcgcact ggatgcgatg atggatatca ctgagaattc cccgcccctg
     9241 cggtagaaca cccagctccc attttccaac ccatcaagac gccttcgcca gctccttcac
     9301 caacggtagc attatccgca taacatcacg gcagcgacgt tctattcttc caggaagtgc
     9361 cttatcaata tgttgttgat tatccagtct tacgtcatgc cagctatttc ccgccgggaa
     9421 ggcaggtgtt tttgcgcgtt gctgataacc atccttattc ccaagattcc agttagtcgc
     9481 ttccaccgaa agtacagcaa tgcccgcttt gtcgaatatt tctgcgtcat tacaacaccc
     9541 agtgcctttc ggataatttt tattcaaacc cggattggtc gttgcggcaa ttccatgact
     9601 acgcgcaatt gccagcgccc tgtcgcgcgt taatttcctt actgcttcag gggtttttac
     9661 accgctgttg aaatacaatt tatcgccaac aattaagtta tcgagattaa tcaccagcag
     9721 cgtatttttc ttttcggtgt cactcatccg cttgagtaaa ttctcagcgc ctaatttccc
     9781 ttcctcttcg ccgctggtcg ccacaaatcg aataccatac tcggtaggcg tatttttcag
     9841 gcgttctgcc aattccagca tgacacctaa acctgcggcg ttatcatcca ttccttgtaa
     9901 cgtcagcccg ccgagattgg catcggcgtc agcatcgctc agcggggcgt aagtatccag
     9961 atgcgccata atgatgatct gctgcggcgc tttgccttca tgagcggcaa tcaccgtact
    10021 tcccgtcacg ttatgccagc tcttacgatt atcgcgggcg gtataaatat accgactatt
    10081 aaatgtccga atatcactgc gataacccat ttgctgaaac tgttggcgaa tataatcggc
    10141 agataacatt tctgctggag ttccagtcat gcgtcccgga aagaaagtag caatatgtcg
    10201 tgcctgggta ttagcaaaat cgccaggttt aggtgacgag gcgtgtacgg ggagaataaa
    10261 gcatacgccg agcgccaggg cagcggtaca gtggcgcaat gcggaaaaca tagtgagtcc
    10321 ttaaatacca tgcaaatttt tttaccgcca tagtatgaaa ctgccgctgc gctaaaacaa
    10381 tttcaaatct tcctaaacgc ccgaaatccg gtgccttaag cactttttga tattagcttt
    10441 gccaaatcgt tattccgtta aggaactact cattctaatt ggtaatttca ttcgttctct
    10501 tacgctccct atagtcgaaa catctgatgg caagaaaata gcggtattgc aaaggaacgg
    10561 ttatggatca aatacgactt actcacctgc ggcaactgga ggcggaaagc atccacatta
    10621 ttcgcgaggt ggcggcagaa ttctcaaatc cggtgatgct ctactctatc ggtaaagatt
    10681 ccagcgtcat gctgcatctg gcgcgcaagg cgttttatcc aggtacgctg cctttcccgt
    10741 tgctgcatgt cgataccggc tggaaattcc gcgagatgta tgagttccgc gatcgtacgg
    10801 cgaaagccta cggctgcgag ctgctggtgc ataaaaaccc ggaaggcgtg gcgatgggga
    10861 ttaatccatt cgtgcacggc agcgcgaaac ataccgatat tatgaaaact gaaggtctga
    10921 aacaggcact gaataaatac ggttttgatg ccgccttcgg tggtgcgcgc cgtgacgaag
    10981 agaaatcccg cgctaaagag cgaatctact ctttccgtga ccgcttccat cgctgggatc
    11041 cgaaaaatca gcgcccggag ctgtggcaca actacaacgg gcaaattaat aaaggcgaaa
    11101 gcatccgcgt cttcccgctc tctaactgga ccgagcagga tatctggcaa tacatctggc
    11161 tggaaaatat cgacattgtg ccgctgtatc tcgctgcgga acgtccggtt ctggaacgcg
    11221 acggtatgtt gatgatgatt gatgacaacc gtatcgacct gcaaccgggc gaagtgatta
    11281 aaaaacggat ggtgcgtttc cgtacgctgg gctgctggcc gctgaccggt gcggtggagt
    11341 caaatgcaca aacactgccg gaaatcatcg aagagatgct ggtttccacc accagtgaac
    11401 gtcagggccg cgtgattgac cgcgaccagg ctggttctat ggagctgaaa aaacgtcagg
    11461 ggtattttta agatgaacac cgcacttgca caacaaatcg ccaatgaagg cggcgtcgaa
    11521 gcctggatga ttgcgcaaca acataaaagc ctgctgcgtt ttctgacctg tggtagcgtc
    11581 gatgacggca aaagtaccct gattggtcgc ctgctgcacg atacccgcca aatctacgaa
    11641 gatcagctct catcgctgca taacgacagt aagcgtcacg gcacccaggg cgaaaagctg
    11701 gatctggcac tgctagtgga cggcctgcaa gctgagcgcg aacagggcat caccattgac
    11761 gtggcttacc gttatttctc taccgagaag cgtaaattta ttatcgccga caccccaggg
    11821 cacgagcagt acacccgcaa tatggcgact ggcgcatcga catgtgaact ggcgatctta
    11881 ctgatcgatg cccgtaaagg cgtgctcgat caaacccgtc gtcacagttt tatctccaca
    11941 ctgttgggga tcaaacatct ggtcgtggcg atcaacaaaa tggatctggt ggattacagt
    12001 gaagagacgt tcacccgtat tcgtgaagat tatctgacct ttgccgggca gctgccgggt
    12061 aatctggata tccgctttgt gccgctctcc gcactggaag gtgacaacgt ggcttcgcaa
    12121 agtgaaagta tgccgtggta cagcggtccg acactgctcg aagtgctgga aaccgtggag
    12181 atccagcgag tggtggatgc ccagccaatg cgcttcccgg tgcagtacgt taaccgccca
    12241 aatctcgatt ttcgtggcta cgccggaacg ctggcatccg gtcgcgtgga agtcgggcaa
    12301 cgtgtaaaag tgctgccctc tggtgtggaa tcaaacgtcg cgcggatcgt gacttttgat
    12361 ggtgatcgcg aagaagcctt tgccggagaa gcgatcaccc tggtgctgac ggatgagatc
    12421 gacatcagcc gtggcgatct gctgctggcg gcagacgaag cgttaccagc tgtgcagagc
    12481 gcgtcggtgg atgtggtatg gatggcggaa cagccgcttt ccccgggcca gagttatgac
    12541 atcaaaattg ccggtaagaa gacgcgtgct cgtgttgatg gcattcgtta tcaggttgat
    12601 attaataacc ttacccaacg cgaagttgaa aacctgccgc tgaacggcat cggcctggtg
    12661 gatctcactt ttgacgagcc actggtgtta gatcgttatc agcaaaaccc ggttaccggt
    12721 gggctgattt ttatcgatcg cctgagcaat gtgaccgtag gtgccggtat ggtgcaggag
    12781 ccagttagcc aggcaactgc tgcgccatct gaatttagtg cattcgaact ggaattgaat
    12841 gccctggttc gccgccactt tccacactgg ggcgcgcgcg atttgctggg ggataaataa
    12901 tggcgctgca tgacgaaaac gtcgtctggc atagccatcc ggtcactgtg caacaacgcg
    12961 agctacacca cggtcatcgt ggtgtagtgc tgtggtttac cggcctctcc gggtccggta
    13021 aatcaacggt tgccggggcg ctggaggagg cgttacataa actcggcgtc agtacgtatc
    13081 tgctggatgg cgataatgtt cgccacggat tatgcagcga tctcggtttt agcgatgccg
    13141 atcgtaaaga gaatatccgt cgcgtcggtg aagtggcgaa tttgatggtt gaagccggac
    13201 tggtggtgct gaccgcattt atctcgccac accgcgccga acgccagatg gttcgcgaac
    13261 gcgtaggaga ggggcgcttt atcgaagtgt ttgtcgatac gccgctggcg atttgcgaag
    13321 cccgcgatcc aaaaggctta tataagaaag cgcgtgccgg tgaactgcgc aactttacgg
    13381 gaatagattc cgtttacgaa gcgcctgaat cggcagaaat tcatctcaat ggtgaacaat
    13441 tagtaacaaa tttggtacag caattattag atctgttgag acagaacgat attatcagat
    13501 cctgagacac caccgggttt ccatgcccgg ttcgtcaaag taacaggatc aaatatgcgt
    13561 aatagccata acattacact aacaaataac gacagcctta ctgaggatga agaaaccaca
    13621 tggtcactgc ctggtgccgt ggtcggtttt atctcttggt tatttgcgct ggcgatgccg
    13681 atgttgattt atggctctaa cacgctgttc ttctttatct acacctggcc tttctttctg
    13741 gcgctgatgc ccgttgcggt agtggtgggg attgcgctgc attcattgat ggacggaaag
    13801 ctacgctaca gtattgtttt caccctgatg acagtaggca ttatgtttgg cgcactgttt
    13861 atgtggctac tgggctaatt tgtactttct tgtctcctct gttcataatt caaaccgtaa
    13921 ctaataatga gattatgttc tgcacgccct gggtatacgt aacaatggac aaatgtggta
    13981 catttgcccg cgttgtcgcg gtatccccaa cagaggatgt agagtcgtct tcggatgcat
    14041 gggatgatga tgccgttttt cagggggcag gatgggtaaa ctaacgctgc tgttgctggc
    14101 tattctggtc tggctacagt attcgctgtg gttcggtaag aacggtatac atgactatac
    14161 ccgcgtcaat gatgatgtgg cggcacagca agctacaaac gcgaaactta aagcgcgaaa
    14221 cgatcaactt tttgccgaaa ttgacgatct caatggcggc caggaggcgc tcgaagagcg
    14281 tgcgcgtaat gaactcagca tgaccaggcc gggcgaaact ttttatcgtc tggtacctga
    14341 cgcgtcgaag cgcgcacagt ctgcggggca aaacaatcga taaatcagcc cgggaattaa
    14401 catggcaacc actcatttgg atgtttgcgc cgtggttccg gcggccggat ttggccgtcg
    14461 aatgcaaacg gaatgtccta agcaatatct ctcaatcggt aatcaaacca ttcttgaaca
    14521 ctcggtgcat gcgctgctgg cgcatccccg ggtgaaacgt gtcgtcattg ccataagtcc
    14581 tggcgatagc cgttttgcac aacttcctct ggcgaatcat ccgcaaatca ccgttgtaga
    14641 tggcggtgat gagcgtgccg attccgtgct ggcaggtttg aaagccgctg gcgacgcgca
    14701 gtgggtattg gtgcatgacg ccgctcgtcc ttgtctgcat caggatgacc tcgcgcgatt
    14761 gttggcgttg agcgaaacca gccgcacggg agggatcctc gccgcaccag tgcgcgatac
    14821 tatgaaacgt gccgaaccgg gcaaaaatgc cattgctcat accgttgatc gcaacggctt
    14881 atggcacgcg ctgacgccgc aatttttccc tcgtgagctg ttacatgact gtctgacgcg
    14941 cgctctaaat gaaggcgcga ctattaccga cgaagcctcg gcgctggaat attgcggatt
    15001 ccatcctcag ttggtcgaag gccgtgcgga taacattaaa gtcacgcgcc cggaggattt
    15061 ggcactggcc gagttttacc tcacccgaac catccatcag gagaatacat aatgcgaatt
    15121 ggacacggtt ttgacgtaca cgcctttggc ggtgaaggcc caattatcat tggtggcgta
    15181 cgcattcctt acgaaaaagg attgctggcg cattctgatg gcgacgtggc gctccatgcg
    15241 ttgaccgatg cattgcttgg cgcggcggcg ctgggggata tcggcaaact gttcccggat
    15301 accgatccgg catttaaagg tgccgatagc cgcgagctgc tacgcgaagc ctggcgtcgt
    15361 attcaggcga agggttatac ccttggcaac gtcgatgtca ctatcatcgc tcaggcaccg
    15421 aagatgttgc cgcacattcc acaaatgcgc gtgtttattg ccgaagatct cggctgccat
    15481 atggatgatg ttaacgtgaa agccactact acggaaaaac ttggatttac cggacgtggg
    15541 gaagggattg cctgtgaagc ggtggcgcta ctcattaagg caacaaaatg attgagtttg
    15601 ataatctcac ttacctccac ggtaaaccgc aaggcaccgg gctgctgaaa gccaatccgg
    15661 aagactttgt ggtggtggaa gatttgggct ttgagcctga tggtgaaggt gagcatattc
    15721 tggttagaat cctcaaaaac ggctgcaata cccgttttgt ggcggatgca ctggcgaaat
    15781 tcctgaaaat tcatgcccgt gaagtcagct ttgctgggca aaaagacaaa catgctgtta
    15841 cagaacagtg gttatgcgct cgcgtgccgg gcaaggaaat gcccgatctg agcgcctttc
    15901 aattggaagg ctgccaggtg ctggagtatg cgcggcacaa gcgcaagctg cgtttagggg
    15961 cgctgaaagg taacgccttt accctggttc tgcgcgaagt gagcaatcgc gatgacgttg
    16021 aacaacgtct gatcgatatt tgcgtaaaag gtgtaccgaa ctacttcggt gcccaacgtt
    16081 ttgggattgg cggtagcaac ttgcagggcg cgctgcgctg ggcgcaaacc aatactccgg
    16141 tgcgcgatcg caataaacgg agtttttggt tgtcggcagc ccgcagtgcg ttgtttaatc
    16201 agattgttgc tgagcgcctc aaaaaagcag acgttaatca agttgttgac ggcgatgcgc
    16261 tacaattagc cggacgtggt agctggtttg tagcaaccac cgaagaactg gcggaattac
    16321 agcgtcgcgt caacgataaa gagctgatga taaccgccgc attgccgggc agtggcgaat
    16381 ggggaactca gcgtgaagcg ctggcattcg aacaagcagc tgtcgccgca gaaactgaat
    16441 tacaaacttt actggtgcgc gaaaaagttg aagccgcgcg cagagcgatg ctgctgtatc
    16501 cgcaacaatt aagctggaat tggtgggatg acgtcaccgt agagatccgt ttctggcttc
    16561 cggcgggtag ttttgcaacc agcgttgtca gggaacttat caacacaaca ggtgattatg
    16621 cgcatattgc tgagtaatga tgacggggta catgcacccg gtatacaaac gctggcgaaa
    16681 gccttgcgtg agtttgctga cgttcaggtg gtcgcccccg atcgtaaccg cagcggcgct
    16741 tcaaattctc tgacactgga atcctccctg cgcacgttta cctttgaaaa tggtgatatt
    16801 gctgtgcaaa tgggaacccc gaccgattgc gtctatcttg gcgtgaatgc tctgatgcgt
    16861 ccgcgcccgg acattgttgt gtccggaatt aacgccgggc cgaatctggg ggatgatgtt
    16921 atttattccg gtacggtagc cgccgcgatg gaaggccgtc atttaggttt tccggcgctt
    16981 gccgtctcgc ttgacgggca taaacattac gacactgccg cggcggtaac ctgttcaatt
    17041 ttgcgcgcac tgtgtaaaga gccgctgcgc accggacgta ttcttaatat taacgttccg
    17101 gatttaccat tggatcaaat caaaggtatt cgcgtgacgc gctgcggtac acgacatccg
    17161 gcagatcagg tgatcccgca gcaagatccg cgcggcaata cgctgtactg gattggcccg
    17221 ccgggcggta aatgtgatgc cggtccgggg accgattttg ctgcggtaga tgagggctat
    17281 gtttccatca cgccgctgca tgtggattta actgcgcata gcgcgcaaga tgtggtttca
    17341 gactggttaa acagcgtggg agttggcacg caatggtaag cagacgcgta caagcacttc
    17401 tggatcaatt acgtgcgcaa ggtattcagg atgagcaggt gctgaatgca cttgccgccg
    17461 tgccgcgtga aaaattcgtt gatgaagcgt ttgaacaaaa agcctgggac aatatcgctt
    17521 tgccgatagg tcaggggcag acaatttcgc agccgtatat ggtggcgcga atgaccgaat
    17581 tactcgagtt gacgccgcag tcgcgggtgc tggaaattgg caccggttcg ggatatcaaa
    17641 cggcaatcct ggcgcatctt gtccagcatg tttgctcggt cgaacggatt aaaggtttgc
    17701 agtggcaggc gcgtcgccgt ctgaaaaatc ttgatttaca taatgtttca acccgtcatg
    17761 gcgatggatg gcaaggttgg caggcacgtg cgccgtttga cgctatcatt gttacggcgg
    17821 caccgccgga aattccaact gcgctaatga cgcagctgga cgaaggcggg attctcgtct
    17881 tacccgtagg ggaggagcac cagtatttga aacgggtgcg tcgtcgggga ggcgaattta
    17941 ttatcgatac cgtggaggcc gtgcgctttg tccctttagt gaagggtgag ctggcttaaa
    18001 acgtgaggaa atacctggat ttttcctggt tattttgccg caggtcagcg tatcgtgaac
    18061 atcttttcca gtgttcagta tggtgccttg cacggtaatt atgtcactgg ttattaacca
    18121 atttttcctg ggggataaat gagcgcggga agcccaaaat tcaccgttcg ccgcattgcg
    18181 gctttgtcac tggtttcgct atggctggca ggctgttctg acacttcaaa tccaccggca
    18241 ccggtcagtt ccgtcaatgg caatgcgcct gcaaatacca attctggtat gttgattacg
    18301 ccgccgccga aaatggggac gacgtctaca gcgcagcaac cgcaaattca gccggtacag
    18361 cagccacaaa ttcaggctac tcaacaaccg caaatccagc cagtgcagcc agtagctcag
    18421 cagccggtac agatggaaaa cggacgcatc gtctataacc gtcagtatgg gaacattccg
    18481 aaaggcagtt atagcggcag tacatacacc gtgaaaaaag gcgacacact tttctatatc
    18541 gcctggatta ctggcaacga tttccgtgac cttgctcagc gcaacaatat tcaggcacca
    18601 tacgcgctga acgttggtca gaccttacag gtggggaatg cttccggtac gccaatcact
    18661 ggcggaaatg ccattaccca ggccgacgca gcagagcaag gagttgtgat caagcctgca
    18721 caaaattcca ccgttgctgt tgcgtcgcaa ccgacaatta cgtattctga gtcttcgggt
    18781 gaacagagtg ctaacaaaat gttgccgaac aacaagccaa ctgcgaccac ggtcacagcg
    18841 cctgtaacgg taccaacagc aagcacaacc gagccgactg tcagcagtac atcaaccagt
    18901 acgcctatct ccacctggcg ctggccgact gagggcaaag tgatcgaaac ctttggcgct
    18961 tctgaggggg gcaacaaggg gattgatatc gcaggcagca aaggacaggc aattatcgcg
    19021 accgcagatg gccgcgttgt ttatgccggt aacgcgctgc gcggctacgg taatctgatt
    19081 atcatcaaac ataatgatga ttacctgagt gcctacgccc ataacgacac aatgctggtc
    19141 cgggaacaac aagaagtgaa ggcggggcaa aaaatagcaa ccatgggtag caccggaacc
    19201 agttcaacac gcttgcattt tgaaattcgt tacaagggga aatccgtaaa cccgctgcgt
    19261 tatttgccgc agcgataaat cggcggaacc aggcttttgc ttgaatgttc cgtcaaggga
    19321 tcacgggtag gagccacctt atgagtcaga atacgctgaa agttcatgat ttaaatgaag
    19381 atgcggaatt tgatgagaac ggagttgagg tttttgacga aaaggcctta gtagaagagg
    19441 aacccagtga taacgatttg gccgaagagg aactgttatc gcagggagcc acacagcgtg
    19501 tgttggacgc gactcagctt taccttggtg agattggtta ttcaccactg ttaacggccg
    19561 aagaagaagt ttattttgcg cgtcgcgcac tgcgtggaga tgtcgcctct cgccgccgga
    19621 tgatcgagag taacttgcgt ctggtggtaa aaattgcccg ccgttatggc aatcgtggtc
    19681 tggcgttgct ggaccttatc gaagagggca acctggggct gatccgcgcg gtagagaagt
    19741 ttgacccgga acgtggtttc cgcttctcaa catacgcaac ctggtggatt cgccagacga
    19801 ttgaacgggc gattatgaac caaacccgta ctattcgttt gccgattcac atcgtaaagg
    19861 agctgaacgt ttacctgcga accgcacgtg agttgtccca taagctggac catgaaccaa
    19921 gtgcggaaga gatcgcagag caactggata agccagttga tgacgtcagc cgtatgcttc
    19981 gtcttaacga gcgcattacc tcggtagaca ccccgctggg tggtgattcc gaaaaagcgt
    20041 tgctggacat cctggccgat gaaaaagaga acggtccgga agataccacg caagatgacg
    20101 atatgaagca gagcatcgtc aaatggctgt tcgagctgaa cgccaaacag cgtgaagtac
    20161 tggcacgtcg attcggtttg ctggggtacg aagcggcaac actggaagat gtaggtcgtg
    20221 aaattggcct cacccgtgaa cgtgttcgcc agattcaggt tgaaggcctg cgccgtttgc
    20281 gcgaaatcct gcaaacgcag gggctgaata tcgaagcgct gttccgcgag taagtaagca
    20341 tctgtcagaa aggccagtct caagcgaggc tggccttttt cttttgtttg ggaagcatgt
    20401 gtttttgtcg cctggataag acacgtcagc gtcctacccg gcaatcagca ttctatgtgc
    20461 tcatcatctt gcgcaccagc tgcataaaca gctcctgctc ttctgcactc aaacgcctca
    20521 aaaattcctc atccacgctg tcaccaatcg gtatcgccgc agcaagtacc ttttctcctt
    20581 cggcagtcag ccagacaaag cgtcgccgct tgtcagctgc gtcatgttca cgtctgacta
    20641 agccacgatt ctccattctt gccaacattt ctgccaacgt tgctttggtg ctgactgctg
    20701 cttctatcag tgccacctgt tcgataccag gcttatcagc aatggcgcgc atcaccgcat
    20761 actgtggttt ggtcaggtca ggtaactcgt gctgccagcg agccgtgtgc tgctgaaaaa
    20821 gctgtcgtaa ctgatggaac gctttatttc gtaacgccat gtaaactccg ggctaattct
    20881 ctgtcgctat catagcggtt ttttagtgac gaagaagatg ttgttacttt tcaatccttg
    20941 ccgctgtgga aatgaaggag tatgttaata ataacgttcg tatacgaaca attaagagag
    21001 taagcaatga aactgatcgt cgggatgaca ggggctaccg gtgcgcctct tggtgtggca
    21061 ttactgcaag cgctgcggga gatgccgaat gtcgagactc atctggtgat gtcgaagtgg
    21121 gcgaaaacca ccattgaact ggaaacgcct tacagcgccc gcgatgttgc tgccctcgca
    21181 gccttcagcc ataacccggc ggatcaggcg gcgaccatct cctcaggttc ctttcgtacc
    21241 gacggcatga tcgttattcc gtgcagtatg aaaacgctcg ccggtatccg cgctggttac
    21301 gccgatggcc tggtagggcg cgcggcggac gtcgtgctca aagaaggccg caaactggtg
    21361 ctggtgccgc gtgaaatgcc gcttagcacc atccatctcg aaaatatgct cgcactttca
    21421 cgcatgggcg tggcgatggt gccgccgatg cctgcctttt ataaccatcc cgaaacggta
    21481 gatgacattg tccaccacgt ggtagcccgc gtgctggatc aatttggcct tgaacatccc
    21541 tacgccaggc gctggcaagg attgccgcag gcccggaatt tttctcagga gaatgaataa
    21601 tggcatttga tgatttacgc agctttttac aggcgcttga tgaccacggc cagttactga
    21661 aaatcagcga agaggtgaac gcggaaccgg atctggctgc cgctgccaac gccaccgggc
    21721 gtatcggcga tggtgcaccg gcgctgtggt ttgataatat tcgcggtttt accgatgccc
    21781 gcgtggcgat gaataccatc ggttcctggc agaaccacgc gatttccttg ggcctgccgc
    21841 caaacacccc ggttaaaaaa cagattgatg agtttatccg ccgctgggat aacttcccga
    21901 ttgccccgga gcgccgcgcc aatccagcct gggcgcagaa caccgtcgat ggtgacgaga
    21961 ttaatctgtt cgatatcctg ccgctgtttc gtttaaacga cggtgatggc ggtttctatc
    22021 tcgacaaagc gtgcgtggtt tcccgcgatc cgctcgaccc ggataacttc ggcaagcaga
    22081 acgtcggcat ctaccgcatg gaagtgaagg gcaagcgtaa gctcggcctg caaccggtgc
    22141 cgatgcacga tatcgccctg catctgcata aagcagaaga gcgcggtgaa gatctgccga
    22201 ttgcgatcac gctcggtaac gatccgatca tcacgctgat gggggccacg ccgctgaaat
    22261 acgatcagtc tgagtatgaa atggcaggcg cgctgcgtga aagctcgtac ccgatcgcca
    22321 ccgccccgct gaccggtttt gatgtgccgt ggggttcaga agtgatcctc gaaggggtca
    22381 tcgaaagccg taaacgcgaa atcgaagggc cgttcggtga gtttaccgga cactactccg
    22441 gcgggcgtaa catgaccgtg gtgcgcatcg ataaagtctc ttaccgcacc aggccgattt
    22501 tcgaatcgct gtacctcggc atgccgtgga ccgaaatcga ctacctgatg gggccagcca
    22561 cctgcgtacc gctatatcaa caactgaaag ccgagttccc ggaagtgcag gcggtaaacg
    22621 ccatgtacac ccacggcctg ctggcgatta tctccaccaa aaaacgctac ggcggctttg
    22681 cccgcgcggt gggcctgcgt gcgatgacca cgccgcacgg tctgggctac gtaaagatgg
    22741 tgattatggt cgatgaagac gttgacccgt tcaacctgcc gcaggtgatg tgggcgctct
    22801 cctcgaaagt aaatccggca ggggatttgg tgcagttgcc gaatatgtcc gtgctggaac
    22861 tcgaccctgg ctcaagcccg gcggggatca ccgacaagct gattatcgac gccaccacgc
    22921 ctgtcgcccc ggacaaccgt ggtcactaca gccagccggt ggtggatttg ccggaaacca
    22981 aagcctgggc tgaaaaactg accgctatgc tggccgcacg taaataagga gaacaagatg
    23041 atttgtccac gttgtgccga tgaacagatt gaagtgatgg cgaaatcgcc ggtgaaagat
    23101 gtctggacgg tatatcagtg ccagcattgc ctttatacct ggcgcgatac cgaaccgctg
    23161 cgccgtacca gccgcgaaca ttatcccgaa gcgttccgca tgacgcagaa agatattgat
    23221 gacgcgccaa tggtgccgag cattccgccg ctgctggcgg aaggtaagcg ctaataaaaa
    23281 gtgccgggta gtggctttgc tttacccggc ctcattactt tattttataa aatgacagcc
    23341 gcccgctttt cggcgatccg gtatcaatat aaatctggtt agcgaacgtc tgaatgttat
    23401 caaacatcat atgtccaaat ataaaataat cagcgccgtt tatttgttgt aactcgccat
    23461 taagcgattt ctgcacacga tcaacaggcc agagtaattc gctctccgct atttctttac
    23521 caaagagata ttcactcccc ggataatctg catgtgcgat gacatatttt atgttgtcgt
    23581 tagttatttc aataatatgt ggaaggtgat ggaatttcag caacagatct attgcctctt
    23641 gttgctctga atcatttaaa tcgaaaaacc agtcaccacc gctggcacgc cacatattgc
    23701 catcgccggt cgcgaacgca tccagcgcca ttgcttcgtg gttgccttta acggagataa
    23761 accagggctg gtttagcagg cgcaggacgt caagactctc cggtccacga tcaatattat
    23821 cgccgacaga aataagtaag tcggtttcgg ggaaaaaaga gagttgatgt aagcgggatt
    23881 gtaataactg atattcacca tgaatatcac caacgaccca tatatggcga tagtgatggg
    23941 cattgatttt ttgatagcgt gtagatggca tggttttacc ctgtaaaata agctttccta
    24001 ttatacaggg tatttttatt tgattcgtca gttatcgtta atattcccga tagcaaaaga
    24061 ctatcgggaa ttattattac accagactct tcaggcgata aatccattcc agcgcctgac
    24121 gcggggtgag tgaatctgga tcgaggtttt ccagtgcctc gaccgcaggc gaggtttctt
    24181 ccggtacgga cagcaaagac atttgtgtac catccacttg cgtagcggcg gcgttcggcg
    24241 aaatgctttc cagctcacgc agtttttgcc gtgcgcgctt aatcacctct tttggcacgc
    24301 ccgccagagc tgcaaccgcc aggccgtagc ttttgctcgc cgcgccatcc tgcacgctat
    24361 gcataaaggc aatggtgtcg ccgtgctcca gtgcatcgag atgcacgttg gcgacgcctt
    24421 ccattttctc cggtaactgg gtcagctcga aatagtgggt agcaaacaac gtcaatgctt
    24481 taatcttatt cgccagattt tccgcgcacg cccacgccag cgacagacca tcgtaagtgg
    24541 aagttccgcg cccaatctca tccatcagta ccagactgta ctcggtggcg ttatgtaaaa
    24601 tattggcggt ttcggtcatt tccaccataa aggttgaacg cccggatgcc agatcatccg
    24661 ctgcccctac gcgggtaaag atgcgatcga taggtccaat ctcaactttt tgtgccggta
    24721 catagctgcc gatgtaggcc atcagcgcaa tcagtgcggt ctggcgcata taggtacttt
    24781 taccgcccat attcggaccg gtaatgatca acatgcgacg ctgtggcgac aggttcagcg
    24841 ggttggcgat aaatggctcg ttcagcacct gttcaaccac cggatggcgg ccttcggtaa
    24901 tgcgaatgcc cggtttatca ataaaggtcg ggcaggtgta gttcagggta taggcccgtt
    24961 ccgccaggtt caccagcacg tcgagttccg ccagcgcgct cgcgctctgt tgcaacgctt
    25021 ccagatgcgg caacagcagg tcgaacaact cttcataaag ctgtttttcc agtgccagtg
    25081 ctttgccttt tgaggtgaga actttatctt cgtactcttt tagctctgga atgatgtagc
    25141 gctcggcgtt tttcagcgtc tggcgacgca tgtagttgat gggtgccaga tggctttgcc
    25201 cacggctgat ttgaatgtag tagccgtgca ccgcattaaa gccaactttc agcgtgtcca
    25261 ggccggtacg ttcacgctcg cggacttcca gacgctccag ataatcggtc gcgccgtcag
    25321 ccagcgcgcg ccactcatcc agctcttcgt tatagcccga tgcgataaca ccaccgtcgc
    25381 gtaccagcac cggcggtgtg tcgatgattg ctcgctccag cagatcgcgc agctcggcaa
    25441 actcgcccat cttctcacgt agcgcctgta ccggtgcact atcgacagtt tctaactgcg
    25501 cacgcagctc cggcagttgc tggaaagcgt ggcgcatacg ggccagatcg cgtgggcgag
    25561 cagttcgtaa agccagacgt gccagaatac gttccaggtc gccgacctga cgcagtaccg
    25621 gctgtagccc ggcggtgaaa tcctgcaatg cgccaatagt ttgctggcgc tcaagcaaca
    25681 cgcgggtatc gcgcactggc atatgcagcc agcgtttcag catacggctg cccatcggcg
    25741 tgacggtgca gtcgagcaca gaagccagcg tattttccgc accacccgcc aggttctggg
    25801 tgatttccag attacgacgc gtcgcggcat ccataatgat gctgtcctgc tcacgttcca
    25861 tggtgatgga acgaatatgc ggcagagtcg tacgttgggt atctttcgca tactgcaaca
    25921 gacaaccggc agcacaaagt ccgcgcggcg cgttctcgac gccaaaaccg accagatcgc
    25981 gagtgccaaa ttgcagattc aactgctggc gcgcggtgtc gatttcaaac tcccacagcg
    26041 ggcgacggcg caggccgcga cggccttcaa ttaacgacat ttcagcaaaa tcttctgcat
    26101 acagcagttc cgcaggatta gtgcgttgca gttctgccgc catcgtttcc cggtcagccg
    26161 gttcgctcag gcgaaaacgc ccggaactga tatccagcgt cgcgtagccg aaacctttgc
    26221 tgtcctgcca gatagccgcc agcaggttgt cctgacgctc ctgcaacagg gcttcatcgc
    26281 tgatggtgcc tggcgtaacg atacgcacaa ctttgcgctc aaccggacct ttgctggtcg
    26341 ccggatcgcc aatttgttcg cagatggcaa cggactcgcc ctgattcacc agtttggcga
    26401 ggtagttttc caccgcatgg taggggatcc ccgccatcgg gatcggctct cccgctgaag
    26461 cacctcgttt ggtcagtgaa atatccagca gttgcgacgc gcgttttgcg tcgtcataaa
    26521 acagttcata aaaatcaccc atccggtaaa acagcaggat ctcgggatgc tgggctttca
    26581 gcttgagata ctgctgcatc atgggcgtat ggtctgataa atccttgtca atagactcat
    26641 tcatattgat tttactcgtt ttttaagtct ttcaagtact caagtggccc caagtgaaac
    26701 cgctttgaac ccatataaat cgataaattt gtagctcact tgtagctcag aacatgatag
    26761 attggcttta ctctttttct gggctacaaa cagtggtgat actttgaaaa caactctcac
    26821 tcagtctttt attatcaaca agttaagtgt ccatgttaag ccagaactca atagctctgg
    26881 gaaagttgtt ttcgaaccta acccacaaca gaaaccatac atcgtttttg acgatcaccg
    26941 tgattcgccg gtaggttttg gtgtaaaggt aagtttgacg aaaaaaacct atgtaatcca
    27001 gagaagagtg tcttctggta ctcgtgatgt gaaagaaggt aagaaaccga gttcggtctt
    27061 aaaagtgaaa gtagggaatg tttctgattt cccgagtatc gatcaagccc gtgaggctgc
    27121 caggcaattg gttcagacca tgatcgccac caaaagaaat ccgaatagga ttaaacgaga
    27181 aacagaagct tcagaactca ctatcagtga agtgtttgct caatatcgtc aacaccttct
    27241 tggcaggtct aaaccagcca aaccaaacac actgtcagtg ttggacaaag cagaaaatcg
    27301 tctgaaagag tggcaagctt tacgagtgaa ggatctcact ggtaatgaca tccttcgtaa
    27361 gtttgatgaa attgcctcaa gagccagaac agcagcggag cagacattca ggtgggccaa
    27421 cgttgcggtc aaacacgcaa tcgaaattga agcggggaat gcgcagacac agcaacggca
    27481 accttcacta tcctacaacc cttttagtat tttaaaggta cagaaaaaat tcaggactcg
    27541 ttctgagctt gaagatagct acaaagcgaa aggtgttcga aacccacttt ctccgaaaga
    27601 cagcttaggt agatttctga ctgcactaca caacaagaga agctttaacc gtttgggttg
    27661 tgattaccta ctgttgacgg ttcttcttgg tgctcgtaaa gaagaaacag cttctctgtg
    27721 ctggcgagaa taccttacta acgaagaagc caaaacgaca agctatgtcg atctcgaaaa
    27781 tcgaaagatt cgattctacg acacgaagaa ccgaaatgat cacgagcttc ctatctgtga
    27841 tgctgtgaaa cgggtgctgg aagacagaag ggacattgtt caggaagcag aaactcgtca
    27901 agaaagacta aaatgggttt tccctgcccg ttccaaaaga agcaaagctg gacactattc
    27961 agatagtaaa agtttgagag agtatctatg tacggaagct gagatcgtca aactcggtat
    28021 gcatgatcta agacgaactt tcggaagatt agcagaagaa cttacttctt atgctgtcgt
    28081 taagaagctg cttaaccacc gcaacacgac agatccaaca gagcgttatg ctgttcccga
    28141 cgaagatcgg gtttacgagg ctctacagcg cattgaattg catatgttga tgactgcgcc
    28201 tacgttatac aatgcacttt tagcatcagc aaaatatcca ccattatctg acaacaagga
    28261 gtaatggatg agtgttgttg aaacattgag agaactgtcc gaagtttggc agttatttgg
    28321 tgatatgcct gatgacacta cgttaggtgt cgatttagcg gcactctacc ttggtataag
    28381 tgtcaaaact cttgcccgtt atcgtcagaa cggtgatgga ccaaactaca tccagtatca
    28441 atctgaagac agtaaagcca gaaaccagcg ggttaattac ctattgaaag accttcgttc
    28501 ctggagagat agccatagag tcagtagcac aatgcacgct gctcaagttc ggggattagc
    28561 atttacatgc ctatctgatt tcaccgagcc tcaacctttc tggcgaatca ccaacaatac
    28621 tggctctaca agtaaaataa tatcccatgc actcactatc cctgatgaaa gctttaagca
    28681 aattctttta gagcctaatg cagaagttat ttggatctct attgaggaag ctctctttgt
    28741 atcatggata aataaagatg aacgttctat ttggcacaag atgtttatct taacactaaa
    28801 taatattgtt tctgcatcgg cggccgaaca ggaaagatat attttgaaag atatctttga
    28861 tgattaatta ttttaattta aatgggatgg gtgggttaag tgcgtcaaat ttaccataag
    28921 gaaaaaataa aaatgaagat aaaggaagca gcggctgttt taagaaagaa tgagtatgaa
    28981 gcatggatca gacgactcaa ctggaaattt atggatgaac gtatccagaa tgaatttaac
    29041 aattgcaagc ttgatgcttc aagtgtagat gagttaggga tgttatattt aggaaag
//
LOCUS       DAZFFG010000054        29095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFFG010000054 DAZFFG010000000
VERSION     DAZFFG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.054"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            198..1064
                     /gene="folD"
                     /locus_tag="ACHJ7J_002638"
     CDS             198..1064
                     /gene="folD"
                     /locus_tag="ACHJ7J_002638"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO5000856.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1066..1278
                     /gene="ybcJ"
                     /locus_tag="ACHJ7J_002639"
     CDS             1066..1278
                     /gene="ybcJ"
                     /locus_tag="ACHJ7J_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO5000857.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1386..1907
                     /gene="ybcI"
                     /locus_tag="ACHJ7J_002640"
     CDS             1386..1907
                     /gene="ybcI"
                     /locus_tag="ACHJ7J_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5000858.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRCFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1943..3328)
                     /gene="cysS"
                     /locus_tag="ACHJ7J_002641"
     CDS             complement(1943..3328)
                     /gene="cysS"
                     /locus_tag="ACHJ7J_002641"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO5000859.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3502..3996
                     /gene="ppiB"
                     /locus_tag="ACHJ7J_002642"
     CDS             3502..3996
                     /gene="ppiB"
                     /locus_tag="ACHJ7J_002642"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO5000860.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3999..4721
                     /gene="lpxH"
                     /locus_tag="ACHJ7J_002643"
     CDS             3999..4721
                     /gene="lpxH"
                     /locus_tag="ACHJ7J_002643"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO5000861.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAGIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4838..5347
                     /gene="purE"
                     /locus_tag="ACHJ7J_002644"
     CDS             4838..5347
                     /gene="purE"
                     /locus_tag="ACHJ7J_002644"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO5000862.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5344..6411
                     /gene="purK"
                     /locus_tag="ACHJ7J_002645"
     CDS             5344..6411
                     /gene="purK"
                     /locus_tag="ACHJ7J_002645"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO5000863.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVNLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6550..7443)
                     /gene="ybcF"
                     /locus_tag="ACHJ7J_002646"
     CDS             complement(6550..7443)
                     /gene="ybcF"
                     /locus_tag="ACHJ7J_002646"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5000864.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7440..8255)
                     /gene="ylbF"
                     /locus_tag="ACHJ7J_002647"
     CDS             complement(7440..8255)
                     /gene="ylbF"
                     /locus_tag="ACHJ7J_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5000865.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8266..9525)
                     /gene="ylbE"
                     /locus_tag="ACHJ7J_002648"
     CDS             complement(8266..9525)
                     /gene="ylbE"
                     /locus_tag="ACHJ7J_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5000866.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9535..11202)
                     /gene="fdrA"
                     /locus_tag="ACHJ7J_002649"
     CDS             complement(9535..11202)
                     /gene="fdrA"
                     /locus_tag="ACHJ7J_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5000867.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11519..12568
                     /gene="allD"
                     /locus_tag="ACHJ7J_002650"
     CDS             11519..12568
                     /gene="allD"
                     /locus_tag="ACHJ7J_002650"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO5000868.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNCEPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12590..13825
                     /gene="allC"
                     /locus_tag="ACHJ7J_002651"
     CDS             12590..13825
                     /gene="allC"
                     /locus_tag="ACHJ7J_002651"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO5000869.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLETQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMDYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13836..14621
                     /gene="allE"
                     /locus_tag="ACHJ7J_002652"
     CDS             13836..14621
                     /gene="allE"
                     /locus_tag="ACHJ7J_002652"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO5000870.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14849..15994)
                     /gene="glxK"
                     /locus_tag="ACHJ7J_002653"
     CDS             complement(14849..15994)
                     /gene="glxK"
                     /locus_tag="ACHJ7J_002653"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO5000871.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNDIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQA
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQKTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16016..17317)
                     /gene="ybbY"
                     /locus_tag="ACHJ7J_002654"
     CDS             complement(16016..17317)
                     /gene="ybbY"
                     /locus_tag="ACHJ7J_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO5000872.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17374..18735)
                     /gene="allB"
                     /locus_tag="ACHJ7J_002655"
     CDS             complement(17374..18735)
                     /gene="allB"
                     /locus_tag="ACHJ7J_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO5000873.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18795..20249)
                     /gene="ybbW"
                     /locus_tag="ACHJ7J_002656"
     CDS             complement(18795..20249)
                     /gene="ybbW"
                     /locus_tag="ACHJ7J_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO5000874.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20418..21296)
                     /gene="glxR"
                     /locus_tag="ACHJ7J_002657"
     CDS             complement(20418..21296)
                     /gene="glxR"
                     /locus_tag="ACHJ7J_002657"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5000875.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETAHQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21396..22172)
                     /gene="hyi"
                     /locus_tag="ACHJ7J_002658"
     CDS             complement(21396..22172)
                     /gene="hyi"
                     /locus_tag="ACHJ7J_002658"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO5000876.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22185..23966)
                     /gene="gcl"
                     /locus_tag="ACHJ7J_002659"
     CDS             complement(22185..23966)
                     /gene="gcl"
                     /locus_tag="ACHJ7J_002659"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO5000877.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRAAAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24056..24871)
                     /gene="allR"
                     /locus_tag="ACHJ7J_002660"
     CDS             complement(24056..24871)
                     /gene="allR"
                     /locus_tag="ACHJ7J_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO5000878.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24949..25431)
                     /gene="allA"
                     /locus_tag="ACHJ7J_002661"
     CDS             complement(24949..25431)
                     /gene="allA"
                     /locus_tag="ACHJ7J_002661"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO5000879.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25661..26587
                     /gene="allS"
                     /locus_tag="ACHJ7J_002662"
     CDS             25661..26587
                     /gene="allS"
                     /locus_tag="ACHJ7J_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO5000880.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YTPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFALAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26656..27750
                     /gene="mnmH"
                     /locus_tag="ACHJ7J_002663"
     CDS             26656..27750
                     /gene="mnmH"
                     /locus_tag="ACHJ7J_002663"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO5000881.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     AYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            27866..28231
                     /gene="ylbG"
                     /locus_tag="ACHJ7J_002664"
                     /pseudo
     CDS             27866..28231
                     /gene="ylbG"
                     /locus_tag="ACHJ7J_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28998..29095)
                     /locus_tag="ACHJ7J_002665"
                     /pseudo
     CDS             complement(28998..29095)
                     /locus_tag="ACHJ7J_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7169 a         7879 c         7234 g         6813 t
ORIGIN      
        1 gtcagttcca gatttacaac gccatcatgg acgaaaaatg aagctttcaa tctcagcgac
       61 ctcagcgacg gtgcgcctaa ccttcgcaag aggcgcttca cgcgataaat ctgaaacgaa
      121 acctgacagc gcgccccgct tctgacaaaa taggcgcatc cccttcgacc tacgtaacag
      181 atggaatcct ctctctgatg gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg
      241 tgcgctctga agttgctcaa aaagttcagg cgcgtattgc agccggactg cgggcaccag
      301 gactggccgt tgtgctggtg ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac
      361 gcaaggcttg tgaagaagtc gggttcgtct cccgctctta cgacctcccg gaaaccacca
      421 gcgaagcgga gctgctggag cttatcgata cgctgaatgc cgataacacc atcgatggca
      481 ttctggttca actgccgtta ccggcgggta ttgataacgt caaagtgctg gaacgtatac
      541 acccggacaa agacgtagac ggtttccatc cttataacgt cggtcgcctg tgccagcgtg
      601 cgccgcgtct gcgtccctgc accccgcgcg gtattgtcac gctgcttgag cgttacaaca
      661 ttgatacctt cggcctcaac gccgtggtga ttggcgcatc gaatatcgtt ggccgcccga
      721 tgagcatgga actgctactg gcaggttgca ccaccaccgt gactcaccgt ttcactaaaa
      781 atctgcgtca ccacgtagaa aatgccgatc tactgatcgt tgccgtcggc aagccaggct
      841 ttattcctgg tgactggatc aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc
      901 tggaaaatgg caaagttgtg ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat
      961 acattacgcc tgttccgggc ggcgttggcc cgatgacggt tgccacgctg attgaaaaca
     1021 cgctacaggc gtgcgttgaa tatcatgatc cacagggtga gtaacatggc gacattttct
     1081 ttaggtaaac atccgcacgt tgagctgtgc gacttgctga aactggaagg ctggagcgaa
     1141 agcggcgcgc aggcgaaaat cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt
     1201 gaaacgcgca aacgctgcaa aatcgtcgcc gggcagacag tgagttttgc aggtcacagc
     1261 gtacaggttg ttgcctgatc cctttcccgg cctgtatcaa caggccggtt ctcttttcag
     1321 acttttgccc tctacgcccc gttcatccat gctgatagca tcgacatttt gccgaagcga
     1381 ccatcatgcc aaccgttatt acgcacgccg ccgttcccct atgcattggt ttagggttgg
     1441 gttcgaaagt catccctccg cgtctgttat ttgccggaat catcctggcg atgctgcccg
     1501 acgcagacgt attgtcgttt aaatttggcg ttgcttacgg caatgttttt ggtcatcgcg
     1561 ggtttaccca ttcgctggtg tttgcgtttg ttgtcccgct gttatgtgtg ctgattggac
     1621 gccgatgttt cagggctggg ctgattcgct gctggctgtt tttaaccgtc tcgttgctat
     1681 cgcacagcat gctggattcg gtaaccactg gtggtaaggg cgttggctgg ctgtggccgt
     1741 ggtcagatga acgctttttc gctccctggc aggtgattaa agtcgcgccg tttgcattgt
     1801 ctcgttacac cacagcgtac gggcatcagg tgattatttc tgaattgatg tgggtgtggt
     1861 tgccggggat gctactgatg ggaatgttgt ggtggcgcag acgataaccg gatgcgaaaa
     1921 ctcgcatccg gcaatagcgc aattacttac gacgccaggt ggtcccttgc gggccatctt
     1981 ccagcacgat ccccatctcg ttaagacggt cacgcgccgc atctgccgcc gcccagtctt
     2041 tcgctttacg ggcatccaga cgctgttgaa ttaacgcttc aatctcagcc acttcgctgt
     2101 cgtctgcctg cgcgccgctt tgcaggaacg cttccggttc ttgctccagc aggcccaata
     2161 cagcggaaag tttacgcagg tgagatgcca ttgcattcgc cgctgccata tcttctgctt
     2221 tcagacggtt cacttcacgc gccatatcaa acagcacgga ataggcttcc ggggtgttga
     2281 aatcgtcgtc catcgcttca ataaagcgcg cttcaaacgc ttcgccaccg gcaggcgcaa
     2341 cggttttatc tgtgccgcgc agcgcagtgt agagacgctc cagcgccgca cgcgcctgct
     2401 tcaggttctc ttcgctgtag ttcagctggc tgcgatagtg gcccgacatc aggaagtaac
     2461 gcacggtttc cgcgtcgtag tatttcagca catcacgcac ggtgaagaag ttacccagcg
     2521 atttggacat cttctcgcgg tcaaccatca ccatcccgga gtgcatccag tagttcacat
     2581 actggccatc atgcgcacag gtggactgag cgatttcgtt ctcgtggtgc gggaacatca
     2641 ggtctgaacc gccgccgtgg atatcaaagt ggttacccag ctgcttgcag ttcattgccg
     2701 aacattcaat gtgccagcca ggacgacccg cgccccacgg agacggccag ctcggttcgc
     2761 cctctttcga catcttccac agaacgaagt ccatcgggtt gcgtttgtcg tcgaccacgt
     2821 caacgcgcgc gcctgcctgc agctggtcga gatcctgacg cgacagcacg ccataagttg
     2881 gatcggtcgg gacgtcgaac atcacgtcgc cgttgtccgc cacataagcg tgacctttgg
     2941 cgatcagttg ttcagtgagt tcaataattt ctgcgatatg gtgcgtcgcg cgcggctcca
     3001 tatccgggcg cagaatgttc aacgcatcaa aatctttgtg catttcggcg atcatgcgat
     3061 ccaccagcgc cacaaagctt tcgccatttt cattggcgcg tttgatgatt ttgtcgtcga
     3121 tatcggtaat gttgcgcaca tacttcagct tatagccgag gaaacgcaga taacgcgcaa
     3181 ccacgtcaaa agcaacaaag gtacgcccgt gaccgatatg acagagatcg taaacggtga
     3241 ttccacacac gtacatgccg acttccccgg cgtgaatagg cttaaattcc tctttttggc
     3301 gtgtcagagt attgaagatt tttagcatcg aagattccgt ttagacatgt gtgggtaatt
     3361 gagttgcgta taatacccat atttcccgcg cgaatcagca tacattgcgt gatgatccga
     3421 tcgtgcggtt atgctataac accaccctat atatgacccg aaccgggttg aagcaccaat
     3481 caaacggaac aggatgcaaa aatggttact ttccacacca atcacggcga tattgtcatc
     3541 aaaacttttg acgataaagc acctgaaaca gttaaaaact tcctggacta ctgccgcgaa
     3601 ggtttttaca acaacaccat tttccaccgt gttatcaacg gctttatgat tcagggcggc
     3661 ggttttgaac cgggcatgaa acaaaaagcc accaaagaac cgatcaaaaa cgaagccaac
     3721 aacggtctga aaaatacccg tggtacgctg gcaatggccc gtactcaggc tccgcactct
     3781 gcaactgcac agttcttcat caacgtggtt gataacgact tcctgaactt ctctggcgaa
     3841 agcctgcaag gttggggcta ctgcgtgttt gctgaagtgg ttgaaggcat ggacgtggta
     3901 gacaaaatca aaggtgttgc aaccggtcgt agcggtatgc accaggacgt gccaaaagaa
     3961 gacgttatca ttgaaagcgt gaccgttagc gagtaatcgt ggcgacactc tttattgcag
     4021 atcttcatct ctgcgtggaa gaaccggcga tcaccgccgg ttttctgcgt tttttagcgg
     4081 gggaagctcg caaggccgac gcgctgtata ttcttggcga tctgtttgaa gcgtggattg
     4141 gcgacgacga tcccaatcca ctccatcgcc agatggcggc agggatcaaa gcggtgtccg
     4201 attccggcgt tccctgttat ttcattcatg gcaaccgtga ttttctgctc ggcaaacgct
     4261 ttgcccgtga aagcggcatg acgttattgc cggaagaaaa ggtgctcgaa ctttatggtc
     4321 gtcgggtgct gattatgcat ggcgacacgc tgtgcaccga tgacgcgggt tatcaggctt
     4381 ttcgcaccaa agtccacaaa ccctggctgc aaacgctatt cctcgctcta ccgttgtttg
     4441 tgcgcaaacg cattgccgcg cgaatgcgcg cgaacagtaa agaagccaac agcagtaaat
     4501 cgctggcgat catggacgtt aaccaaaacg cggtggtcag tgcgatggaa aaacatcagg
     4561 tgcaatggct gatccacggg catacccatc gcccggcggt gcatgaactt atcgccaatc
     4621 agcaacctgc ttttcgcgtg gtactgggtg cctggcatac ggaaggttca atggtgaaag
     4681 tcacggcgga tgacgttgaa ctgattcatt ttccgtttta aatacccgcg actttgctga
     4741 tttcacagcc acgcaaccgt tttccttgct ctctttccgt gctattctct gtgccctcta
     4801 aagccgagag ttgtgcacca caggagtttt aagacgcatg tcttcccgca ataatccggc
     4861 gcgtgtcgcc atcgtgatgg ggtccaaaag cgactgggct accatgcagt tcgccgccga
     4921 aatcttcgaa atcctgaatg tcccgcacca cgttgaagtg gtttctgctc accgcacccc
     4981 cgataaacta ttcagcttcg ccgaaagtgc cgaagagaac ggttatcagg tgattattgc
     5041 gggcgcaggc ggcgcagcgc acctgccagg catgattgcc gccaaaacgc tggtgccagt
     5101 gctgggcgtg ccagtacaga gcgccgcact gagcggtgtc gatagcctct actccatcgt
     5161 acaaatgccg cgcggcattc cggtgggtac gctggcgatt ggtaaagctg gcgcggcaaa
     5221 cgcggcgtta ctggcagcac aaattcttgc gactcatgat aaagaactgc accagcgtct
     5281 gaatgactgg cgcaaagccc agaccgacga agtgctggaa aacccggacc cgcgaggtgc
     5341 ggcatgaaac aggtttgcgt cctcgggaac gggcagttag gccgtatgct gcgtcaggca
     5401 ggcgaaccgt taggcattgc tgtctggcca gtcgggctgg acgctgaacc ggcggcggtg
     5461 ccttttcaac aaagcgtgat taccgctgag atcgaacgct ggccggaaac cgcattaacc
     5521 cgcgagctgg cgcgccatcc tgcctttgtg aaccgcgatg tgttcccgat tattgctgac
     5581 cgtctgactc agaagcagct tttcgataag ctccacctgc cgaccgcacc gtggcagtta
     5641 cttgccgatc gcagcgagtg gcctgcggtg tttgatcgtt taggtgagct ggcgattgtt
     5701 aagcgtcgca ctggtggcta tgacggacgc ggtcaatggc gtttacgcgc cgatgaaacc
     5761 gagcagttac cggctgaatg ctacggcgaa tgtattgtcg agcagggcat aaacttctct
     5821 ggtgaagtgt cgctggttgg cgcgcgcggc tttgatggca gcaccgtgtt ttatccgctg
     5881 acgcataacc tgcatcagga cggtattttg cgcaccagcg tcgctttccc gcaggccaat
     5941 gcacagcagc aagcgcaagc cgaagagatg ctgtcggcga ttatgcagga gctgggctat
     6001 gtgggcgtga tggcgatgga gtgttttgtc accccgcaag gtctgctgat caacgaactg
     6061 gctccgcgtg tgcataacag cggtcactgg acacaaaacg gtgccagcat cagccagttt
     6121 gagctgcatc tgcgggcgat taccgatctg ccgttaccgc aaccagtggt gaataatccg
     6181 tcggtgatga tcaatctgat tggtagcgat gtgaattatg actggctgaa actgccgctg
     6241 gtgaatctgc actggtacga caaagaagtc cgtccgggcc gtaaagtggg gcatctgaat
     6301 ttgaccgaca gcgacacatc gcgtctgtcc gcgacgctgg aagccttaat cccgctgctg
     6361 ccgccggagt atgccagcgg cgtgatgtgg gcacagagta agttcagtta acctggtgct
     6421 catttgattg ccggatgcgg cgtgaacgcc ttatccggcc tacaccgctc cggtacccat
     6481 tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat ggtgcaccga ttgctggatg
     6541 ctctcatcac tacagcgaaa tacaggtccc cgcttcgccc gccagcgtct cttcaattcg
     6601 cgataacgcc ccaatccacg cgggtttacc gcggcttctg acataaccac tcaccgccgt
     6661 taccttcggc cccatcgaac catcggcttt ggcaaatggc gctaactcat ccggtgtggc
     6721 atggcgaatg gcacgttgct gcggcgttcc ccagttttca tataccgcat cagcatcggt
     6781 gaggatcacc agtccatctg cattaatctg ctcggcgagc aacgcagcgg cgagatcttt
     6841 atcaatcacc gcttcactcc ctgctccgtc atccgtcaca ggcacaccgc cgccgccgct
     6901 gcaaatcacc acatgcccct ctttgagcaa caactcgatg gcttcgctgt cgagaatttt
     6961 acgcggttgc ggagacgcca ccacccggcg caaatattta ccatcacgtt tcatctgcca
     7021 gccgtaagcc gcttccagtg cctcttgttc ttctggctga taaaccggac caataaattt
     7081 ctctggctgc aaaaacgccg gatcatcagg cgaaacttca atgcgcgtca gcaccgtcgt
     7141 gacaggcggc atctgcggct gtgcgctcaa actctgcgcc agcatatagc caatcatccc
     7201 ctgactttct gccaccagca catccagcgg atacggttct acctctttcc acgccagatt
     7261 ctgcaatgcc agcaacccca cctgcggccc gttgccgtga acaatcgcca gccgataaga
     7321 acgggccagg cgcgccagcg cgggtacagc actggcgata ttgcgatatt gattttctgc
     7381 cgtcagcgcc tcaccgcgct ggagtaaggc gttgccccca agagctacaa ccagtgtttt
     7441 catggttttc cttgtaataa ttgttggcca agccaaaacc ccagcagcgt atctgcccct
     7501 gacgtatgcc ccagcgccag cagcgaatca atcgcaacag cggtacgttt cgggcaactc
     7561 agagcatgaa caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa
     7621 taactgacgc tcaccgctgt ggtaacgagt tgcagattgt cggaacaggc aaaaaacgga
     7681 cgaccggagc gcgcatctaa agcgccataa taccaggccg ccagcagcat tccgctcagc
     7741 gtgtcatcat gactcggcgt taatccgggg cctttaccca gccagtgccg ccagtcggtc
     7801 ttaacgccat tgagcgcggc ctgaaaacag tgacgaaact ggcgtaactc agcaggcagc
     7861 ggatcgcttg ccgccaacgc cagtggcccg aaaagcccgg tttcctccgc gcgttgcatc
     7921 catgcagctg caagtggttg aggatgcgca ggcggcgtaa tacgcagcaa acaataacgc
     7981 tgtggctgtt taaccgtgaa acgcccgagg cgaatccctt gagccacaac ctgtggtcgt
     8041 tcattgccgc ataatccacc gcataacgca tcgaattgcg cacggcgaag cacccatcct
     8101 ccggggccga aaccactacc ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc
     8161 aggttaatcg cccgccgcca cactcccgct aaccgccagg actggcgata attcggtgcg
     8221 ctactactgg caagcagagg atggatgatc gtcattgcgc gttcctcaac caatccccat
     8281 gctttcagcc agtgcttcca gcgcctgttc aaaacacgcc agcggtgccc gcacggtgcc
     8341 tgcgccaatc tgcccgatcc ccgcctcttt atgggcgata ccggtattga tgagtggcgt
     8401 aatgccggtt tctaccacgc gacgaatgtc cagtcccagg cacgcgccct gaaaatccca
     8461 gcttgggatc tgcaactgca tattgcgttc aaggtaaatt tccgccatct cttcagatac
     8521 cgccctggcc gcttccatgc cacccgcacc gacaaagcgc gttacgccag gcgctgcgat
     8581 cattgccgca cctccgatac caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt
     8641 cgcctgctcc tgcgagaagc cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt
     8701 aaaccagcgt tcgcccagcc cgcttacccg aatcccgaac atattgccgt tgcgggtcat
     8761 tgccgtgacg atgctgcctg cgcggatcat cgcgccagca tccatcgccg ccttgcagta
     8821 agccatcgcg aggttgagga agaactgatc ggtcacgctg aggaaatcca tcacttccgc
     8881 gatgtgctgt ttatcatgat cgaggcgagc aatttgtggg gccagcgcac gcattaacag
     8941 tgctgaggaa gcaatattgc gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat
     9001 catcgcagta agatcgatac cgcgctccat gcgccccagc gccgcgctta atactggcat
     9061 taacacatcg cgcatccagc gatggcgagt caggacatct tcgccgtaag cgccaaaacg
     9121 catcactttg ccgatacctt cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt
     9181 ctcgaccacc agcatcggca tactggcaga agtaataccg cccattggcc cgacggcatt
     9241 cacatggtga caaggaatga agttcacttc cccctgctcc agtattgcca gcgcctgcgc
     9301 ttcatctttc gcccaacctt cgaacagaca tgcgcccacg cacgcccctt tcatgggtcc
     9361 ggtcatctcc tgccagcgca ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag
     9421 ttcgctgata agtgaagaag ccggttgcac atccagccag tgtggacgag cgcgacgaat
     9481 ttgttcgata accgcagcat tggcttgcgc cactgatgta aacatgagaa ccccttattg
     9541 caaacgttct aataaacgag ccagtttttt attgccgccg gcgactggcg accattggta
     9601 atgcacaacc ggtttgctgg cgctttgtag ctccagcgca aaactgcgta atccgatgtt
     9661 aatcacggcg acgttttcca gtaatgacgg tgtgtgttgc tgtgcggcag gcgagagcgg
     9721 atgaattaac gctgccgcca gcaaggtggc ttccggtagc gaactcacga ccgcaatccc
     9781 cgcatcttcc agcgtggcga tttgctgaga gcggcattgc gggtcacgtt cagtgcctgt
     9841 caccgtggca atggcataca gtggttgatt gtctgaacgc gcagcacagg ctttttgcca
     9901 ggcgctcacc agcgaggcgg caggatcggc ggtcgcaccg aagccaatca cgacatcaag
     9961 cagcagcaca cgcacttgcg gtttagcgcc gagatcggca attaactggt tgcgtaaggt
    10021 tgggtcgatc atcggatggg gacgcccgac tgtgtagaaa tcatcgccaa ggtcaataat
    10081 ctggtgacca tcggcgtcga gcatcatgcc gtgatgatgg gcatcatcgg cttccacgcc
    10141 aaggtgtccg gcaagtaatc ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc
    10201 gcaaataaat ccgctgctgg caggcgttat cgcgttacgt cgcgccgtaa cgcgtgaaag
    10261 caggcaagcc aggcgtgcgg cctcatccag cgaggaggca aaccagacat tctcgtcgcg
    10321 ggccaccgcc ggggtataac ctaaaaacag cgccaccgtc ggtttgccgg ttgctttcat
    10381 ggcattaaca attttcagac gcaccgcttc ggcaggtggt tttgaaacaa atgccagcac
    10441 ttcgcttttc tcgtctgcac tgagcatttc cagcgccgtc agcgcactga tgccgcccac
    10501 ttcacggctg aggtcgcggc cgccaaggcc aatcgcgtga gtaattccct cccctgccag
    10561 cgcaatctgc gagcacagct cctgaatccc ggtaccggaa gcgccaatga cgccaatgtt
    10621 gccttccggc atcacgttag caaaagccag cggcgtgccg gcaatcatcg acgtaccgca
    10681 gtccggcccc atcaccagca agcctttttc ccgcgcgcgg gttttaagtt ggatttcatc
    10741 ttccagcgtg acgttatcgg agaacatcat cacgttgagg ttgcgatcca gcgcctgatt
    10801 cgccagctcc gccgcatact cgccagccac tgaaatcagc gccagattgg catcgggtaa
    10861 tttctgacag gcactgtccc agcgacgcac ctgcgtcaac gcctggctgc tgcctgaccc
    10921 ctgcgccagt tgttttagcg cctcttcaag ctgctgcata atcgcctgcg cgatccccgc
    10981 atccgccgct tcgctacgaa ttgccacgca aatatcgttc ggcgtggcgt tattaaaatc
    11041 gtcatgccag aaacctgtgg tatctaataa cgctttattg gcgggcgtac ccatcattac
    11101 ggaaacatca tcaacatttt ctgattcgct gagttttcgt gaaataatca ttaaactgac
    11161 cgaatcctga aaacaccctt ttttaataaa ggcgtggatc ataacaactc cttaaaaatt
    11221 gcacccatgg caattgcacg agcagtgtta atgtggcgat tacggtatcg atgacaacga
    11281 gtaaataagg tgatgaatat cacacaggga ttattaacaa aagaattcaa ataaccgcca
    11341 gactattacg cgttattttt taataagcat ggttgtttta aaaaccaggt taaaagcgcg
    11401 aagattatca aataaattct aactatattt ttttgcctgt ctggatcaca taatccagat
    11461 attttccctg tatgttaatt gcagtcatgc ttcacaccgt cgttaaaaag gaagacagat
    11521 gaaaatcagt cgggaaacac tccaccagct aatcgagaat aaactctgcc aggctgggtt
    11581 aaaacgtgag cacgctgcaa ccgtggctga agtattggtt tacgccgatg ccagagggat
    11641 ccactctcat ggcgcggtgc gcgtggaata ctacgcggaa cgcatttcaa aaggcggcac
    11701 caactgcgaa ccggagtttc gtcttgagga aaccgggccg tgttcggcaa ttttacatgc
    11761 cgacaatgct gccggacagg tcgcggcgaa aatgggtatg gaacatgcca tcaaaaccgc
    11821 ccagcaaaat ggcgttgcgg tggtcggtat cagccggatg ggtcacagcg gcgcaatctc
    11881 ttattttgtg cagcaggcag cccgcgccgg attaattggc atttcgatgt gccagtccga
    11941 tccaatggtg gtgccgtttg gcggcgcgga aatttactac ggtactaacc cactggcctt
    12001 tgccgcgccg ggagaaggcg acgagatcct tacctttgat atggcgacta ccgtacaggc
    12061 atggggaaaa gtgctcgacg cccgctcgcg taatatgtct atcccggata cctgggcggt
    12121 cgataaaaac ggtgcaccaa caaccgatcc gttcgcggta catgctctgc tccccgccgc
    12181 tgggccgaaa gggtatggcc tgatgatgat gattgacgtt ctctcaggcg tcttactcgg
    12241 cttaccgttc gggcgacagg ttagttcgat gtatgacgat ttacacgccg ggcgtaattt
    12301 ggggcaatta catgtagtta ttaacccgaa ctttttctcc tccagcgaat tattccgtca
    12361 acatcttagc cagaccatgc gcgaattaaa tgccattacc cccgcgcccg gttttaatca
    12421 ggtttattat cccggacagg atcaggatat taaacaacgc aaagccgccg tcgaaggcat
    12481 cgaaattgtt gatgatattt accagtattt aatttccgac gcgctttata acacgtcata
    12541 cgaaacgaaa aatccctttg cgcaataatt attgagacag gactttctta tgattacaca
    12601 ttttcgtcaa gccatagaag aaacgctacc ctggctttcc tcatttggcg ctgacccaac
    12661 gggtgggatg acccgtttac tttattcgcc ggaatggctg gaaacccagc agcaatttaa
    12721 aaaaagaatg gccgcaagtg ggctggaaac acgtttcgat gaagtgggga atttatacgg
    12781 tcgcctgagt ggcaccgaat atccacagga agtggttctg agtggttcgc atatcgatac
    12841 cgtggttaac ggcggtaacc tcgacgggca attcggcgcg ctggcggcgt ggctggcaat
    12901 tgactggctg gaaacgcaat acggcgcacc gctgcgtacg gttgaagtgg tggcgatggc
    12961 ggaagaagaa ggcagccgct tcccgtatgt tttctggggc agtaaaaata tctttgggct
    13021 ggcgaatcct gacgacgtgc ggaatatctg tgatgccaaa ggaaatagtt ttgtcgatgc
    13081 gatgaaggct tgcggattta ctctgccgga cgccccgcta actccgcgtc aggatattaa
    13141 agcctttgtc gaactgcata tcgaacaggg ctgtgtgctg gaaagtaatg ggcaatcaat
    13201 tggcgtggtg aatgcaattg tcgggcaacg tcgctatacg gtgacgctga acggcgaatc
    13261 aaaccatgca ggcaccacgc cgatggatta tcgtcgtgat acggtttacg ctttcagtcg
    13321 catttgccat cagtcgatcg aaaaagcgaa aaagatgggc gacccgttgg tgctgacctt
    13381 tggtaaggtg gagccgcgcc cgaatacggt gaatgtggtg ccgggtaaaa ccacgttcac
    13441 cattgattgt cgtcataccg acgccgccgt gctgcgtgat ttcacccaac agttagaaaa
    13501 cgacatgcgg gcgatttgcg atgaaatgga cattggtatt gatatcgatt tatggatgga
    13561 cgaagaaccc gtgccgatga ataaggacct ggtcgccacc ctgacagaat tgtgtgaaag
    13621 tgaaaaactg aattaccggg tgatgcacag tggtgccggg cacgacgcgc aaattttcgc
    13681 gcctcgcgtg ccgacctgca tgattttcat tcccagcatc aacgggatca gccataaccc
    13741 ggcggaacgc accaatatta ccgaccttgc cgaaggggtc aaaacgttgg cactcatgct
    13801 ttatcaactt gcctggcaga aataaggagt cataaatggg atatttaaat aacgtcaccg
    13861 gttaccgcga tgatttactg gctaaccgtg cgattgtgaa acacggtaat tttgcactgt
    13921 taacaccaga cggtctggta aaaaatatta ttccgggctt tgaaaattgt gacgcgacaa
    13981 tcctctccac gccaaagctg ggtgcctctt ttgttgatta tctggtcaca ctgcatcaaa
    14041 acggtggcaa ccaacagggc ttcgggggcg aaggcattga aacgttcctg tatgtgatct
    14101 ctggaaatat cactgccaaa gccgaaggca aaacatttgc cttaagcgaa ggtggctatc
    14161 tttattgccc gccaggctcc ttaatgacgt ttgttaacgc ccaggccgaa gacagccaaa
    14221 tctttttata taagcgccgc tatgttccgg tagaaggcta tgcaccgtgg ctggtttctg
    14281 gcaatgccag cgaactggaa cgcattcact atgaaggtat ggatgatgtg atcctgctgg
    14341 atttcctgcc caaagagtta ggttttgata tgaacatgca tatcctctct tttgcgccag
    14401 gtgccagcca tggttatatc gaaacacacg ttcaggaaca cggtgcctat attctttccg
    14461 gtcagggggt ttataacctc gacaataact ggatcccagt gaaaaaaggc gattacatct
    14521 ttatgggcgc ttattcttta caggctggtt atggcgtagg gcgtggtgaa gcgttcagct
    14581 atatttactc gaaagattgc aaccgcgacg tagagattta agtcatacca ctcgcctgat
    14641 tatttttagc tatgttggat tttgccacgg tttataccgt ggcttttttt gtaacattgc
    14701 ctgatgcgct tcgcttatca ggcctacatg atctctgcaa tatatagaat ttgcgcgatt
    14761 ttcaggaagg atatagtgct catgcagcat ccggcatgag cactgcgctc ttatacgaaa
    14821 taaatcgcat ccgctttaaa ggtaagagtc agtttttaat tccctgacct attttaatgg
    14881 cgcaggcaat atttcgcgcg ctgttgaaga gattggtttc accgctggcg agcacttcgg
    14941 ctaaaggtgc cagacgtggc aaaatgctga ataccgcatc aatgccgtac tggtgcacca
    15001 cttccacgcc atcacccaat acgccagcaa tcccaatcac cgggacatta aactgcttcg
    15061 ccaccgacgc aacgcccagc ggcgctttac cgcctgccgt ttgcgagtcg atgcgccctt
    15121 ccccggtgat caccagtgct gcgccctgca ccgcctgctc caggttgacc gcccgcagta
    15181 caatctcaat acccggttta atatccgcat tgaggaatac cgccgccgca atccccatac
    15241 cgcccgcagc gccgccgccc gccatctgac agacattaat ttcagttttc tgttgtaaaa
    15301 cacgggcgta attttgcagc ccctgttcca gctcttcgac catctccggc gttgcccctt
    15361 tttgcgggcc aaacaccgcc gccgcgccgc gtgcccctac cagcgggtta tcaacgtcac
    15421 atgctacttc aatatggcaa ttcgccagac gcggatcgca ttcctccatc tcaatgcttg
    15481 ccacgcgcgc taaattacca ccgtttgccg ccagcgcctg accgtcggca tcaaggaaac
    15541 gcacaccgag cgcctgcgcc atgcccatac cgccgtcgac cgtcgcactg ccgccaatgc
    15601 cgagaataat atggcgaatg tcgttatcca gcgcatgccg aattaactcc cccgtaccaa
    15661 aactggaggc cagcaacgga ttacgctttt caggcgcgac cagcatcagg ccacttgctg
    15721 ccgccatctc aatcaccgct gttttcccgt cgccggtaag gccataaaaa gcattcactt
    15781 tttcgcccat cggcccgcag acttcaagcg tcacgatgtt accacccgtc gcggcgacca
    15841 tcgcatccac cgtcccttcg ccgccatccg ctatcggcaa acagatatag ttcgcatcgg
    15901 gaaagagggt cgaaaacccg gctttaattg cctgacaaca tttttctgca cttaagctct
    15961 ctttaaaaga gtctggcgca atgacaatct tcattatcaa tcctttttca acaaattatt
    16021 cgatgcgttc ccacggaata aggttatcca taagaacagc cagtaaaatc ccgaccaata
    16081 agccgttact gagcagagga cgaagcgtta atggaaggtc ttgcagatac acaggcggta
    16141 atgccattaa aaatatgccg acaaataacg gcaatgcgag tcgataaata ttgcgagcgg
    16201 taaacgttat ttgctggcta aacaccagcg cggaaaagag taaaggcaga taagaaacca
    16261 gcatgaccgc actactcacg ggtaaaggga tactgcaaaa cagtcgcgtg agtgccggaa
    16321 ccagcgccac cagcaggcaa ataacgctgc cataaataaa cgaacgccgc gtgtaatcgc
    16381 cagtctgggt taataaacca atggatgaaa caaacggtga aaatggaatt actgccagcg
    16441 gcacggttat cagtgtcata aatccggtcg ccacaaagct acgacgataa cgcgtattcc
    16501 ccgcgccctg ctgcggataa aaaacatccg tgccccgaat cgcaccgtag gtattgctga
    16561 tattcaccag acctgtaatc accgccgtca gaataatccc cggagacaaa gcaccgccac
    16621 tgccgagcgg gaaccactgc cagtgcaact caccggagag cgaatgcgaa gaaggaaagc
    16681 aaaagtacca caacaaccag ccggttatgg tgccgaccag caggccataa cgggcaaaac
    16741 gttgcggcag gaaaataatc atcgccagta ccaggcacat caccgccacc gagagcgcga
    16801 acggcggtaa ctgaatttta aaattcgggt cggctatgcc aaacggcagc ccgagcatac
    16861 ctttgaaaaa gatagtggtc agctgcgcgc ccagcatcaa cataaacaag accatcaccg
    16921 acggcgtaaa taaccgcgcc aggcgatgac ctaatccgct aaaaccaatc aacatcgtca
    16981 gcacgccgga gagtgcaatc cccaccgcca ggctggtagc gatatcgttg atcggtgtcc
    17041 cgcgcgatgc ttcgccaagg gtgatagtta ggatggttcc ccaccacagg ccccccggcc
    17101 cttccataat agcgcgacga tgaccgcaaa acgcctgagc gaagcaggcc agtgcggtag
    17161 caagaaaagc atattgtgtg agtgtaagca ggctactttg cggcaactga aaagcagaaa
    17221 gtagcgtagg aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac tgaaatcctg
    17281 ataacaggct ttcgcggctg actgcaaaat tgaacataaa cacaccttaa ccggagaatg
    17341 cccgccgcaa ggacgggcat tgcaggggcc agattactgc tgatgtttaa ggataaattg
    17401 acctttcggc gcaacaggga agccctgttc aatgtcgtaa atcacatcac cacgtaagat
    17461 ggttttcgtg atacgcgcgc caatggtacg gccaacatac gggctgactt tgtggcgata
    17521 ttccagatcg tcattggtaa gaacatagct gctattcggc tgaatgaaga cgaagtcggc
    17581 atcttttcct ggggcgatac ggcctttttg ctgcagaccg aaaatatctg ctgcgttagt
    17641 cgccattaat ttgccgaaca ttggcagaga cattccgcgt ttctgtaccg cttcatcgaa
    17701 catcacgtcc atacaatttt gcagaccggc aataccgccc catgcttcca tgatgttgcc
    17761 ggctttcatt tccggagggc atggtgagtg gtcggaaacc aggcagtcga tttcaccgtt
    17821 aaacagtttt tcccacatgc ctttctggtt ttccagatcg cggatcggcg gtgaacactt
    17881 cgccagagta ccgatttctt cgaactgatc ggtatccagt acaaagtaat gcgggcagga
    17941 ttcacaagta acgtcctgac cttcctgacg tgcacgagtc acttcttcaa cgccttccgg
    18001 gctgctgata tggcaaacgt gcagacggca accggcaact ttcgccagat acagcacgcg
    18061 gcgaatcgct tccacttcgg taaataccgg acgcgaagcc acatagtcat gggcagttac
    18121 gcgaccttca cgtttcgctt cttcgcccag tgcgtcacag atcagcgcgt tttcgcagtg
    18181 caccagcacc ggctgcccca gttcgcccag cttctgcgca cctttgaaga actgccagtc
    18241 gtttacatca cggaagtcgt tgtcgatacc gcgatcgcca caggtcgcaa cgaagcattt
    18301 gaagccgaca acgcccactt catccagctc atgcagacga tcaatgttgt aagataccag
    18361 gccaccgagt tgtgccgcat cgatagtcag cttgccttta gcggcatcga acttcagttc
    18421 aattgaagcg cggtcaaccg ttgcaggcag ctggttgagc ggcatttcga tcatggtggt
    18481 gataccacct tttgccgctg cgcgagtacc ggtttcataa ccttcccagt ggctacgacc
    18541 cggttcagaa atatgggtgt gcgcatcaac catgcccggc gaaaccacca gaccagacgc
    18601 atccataact tcttttgcat cgcccagatc ctgaccgata gcagcaattt tgccgccttt
    18661 aacggcgata tctacaacgc gagcttcgtt ttctaaaata acggtgccgt ttttaatgat
    18721 taaatcaaaa gacataacaa actccttgta aaatacagat tggtttttaa ctcaatgttc
    18781 aatatccgga ttaattaacc tatggttttt tgctctcctg ttttttctgc tgttgtacgt
    18841 ttctttaata aggcgtaggc cgcaaaggcg acgatgacgc cgacaaacca tgaaacacgc
    18901 gataacggtt ccataaaggg aataaactta ccgccaagag ataaaataac ggccaccaga
    18961 gttactgaaa acgcggtgag gttaaaaccg ttatcgtaat atttataatc gccaggtgcg
    19021 gtgtacagtt catcaagatt aatttgccca cgcatcacca caaaataatg cgccatcatg
    19081 acaccaatta ccggaccaag cattccgccg ataatatcga ggaacagata aatactgtcc
    19141 tgattttcca ttaatttcca cgggcagatc agcaggctga taatactggc aatcagtacg
    19201 ccgtttttat aggtcagttt tgtcggtgca atggcagcaa tctgataacc ggccggaata
    19261 atattaccgg tggcgttagt ggagatagtc gtcatcagaa taaccagtac cgcaaagaac
    19321 gaggcgaaca agctgtccca acgctgaaca atatccagca cgttccaggt gtcagcgccg
    19381 tagtgaatac tggctccggc aataatacat accccggcga ccgcaaacag aatataggcc
    19441 acaactaaac ccagcgtttg ccccagcgct tgctcacgaa acgagtgggc gttttgcgta
    19501 aagtcggatg cgctcaccgc cggtgccgcc cagaccgcaa ctaccgcgtt aatcaccacc
    19561 aggaacaaga atccactgtt ttctgctttc tgaataccgc tcggaatgta gtcaaagatt
    19621 ggaccgagcc cgaccagtga aatcgcccaa atcgccatac cgccgaaaac gatatagatg
    19681 cacgggttaa gaatggcagt gaatttattt aaaactttgc caccgccaaa acctatacca
    19741 acgttgacca gccagaagag taagaaagta attaagcccg gtagagaaag gcctaacaga
    19801 gtgaaatcac caccgagagt caaaaatccc ggccagattt tgccaatcag aatcaagcag
    19861 gccagtgacc ccgcgtaaca ttgcaggcca aaccacataa tggcagcaat accgcctctt
    19921 aataatccgg gaaacagtgc gccacgtacg ccgtaagaag cacgcaggat catggcaaac
    19981 ggtacgccgt atttactgcc cgcagcaccg tttaatacca ttaccgcggc aatgaaaaag
    20041 gcgctgagga taattgccag cataatacta aaggtagaca agccgagaat aaaaaagccg
    20101 ccgaccatca cataattggg aacgttatga accgaaccca tccataaggt aaaatagtta
    20161 aatgttttcc aggtccgctg gctttgcgtt ttcggcaata gatcttcgct atagccgcgt
    20221 tgctggaata gttttctctg atgttccata aatccctctt aatttcacgt atcgaataac
    20281 gttgttttaa tctagctatg aggaacaaat aaaataaaaa tattattttt aatactgaag
    20341 caacatcttt ggctgctttc ttttttaata ttgctggccg attcattttc tgatatcggc
    20401 catttttatt gcgggtatca ggccagttta tggttagcca ttaattccag cgcctgcact
    20461 aacgcagagt gatccaactg gctgccaccg tttgccgcac aggtattaaa taactcctgg
    20521 caggtcgcgg tgtttggcag gttcagcgcc agtgccttcg cactttgcag tgccaggttg
    20581 agatctttct ggtgcagcgc aattttgaaa cctggattaa aggtgcgttt aatcatgcgc
    20641 tcaccgtgaa cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc ctggcgcaca
    20701 cgtaccgggt ctgcaccggc ttttgaagca aacagcagag cttcagagac cgcttcaata
    20761 ttgagcgcta cgataatctg atttgccact ttgcaggttt gaccgtcgcc gttaccgccc
    20821 acaagggtga tatttttacc gagcagttca aacagcggtt taacacgttc aaataccgct
    20881 tcgtcaccgc caaccataat tgacaacgtc ccttcacgcg cgccgatttc gccgccggag
    20941 actggcgcat cgagataatc accgcccagt tcattcacct gacgagcgaa acgtttagtt
    21001 tcaatcgggg aaatggagct catatcaaca atagttttgc ccttcagcga ggctttggta
    21061 caaccatttt caccgaacag aacttcttca acctgtggtg tatccggcac cataataaaa
    21121 atgatgtccg atgcttccgt tacctggtgc gcagtttcaa cactgacggc acccagtgac
    21181 agtaattcat cagcaaccgg accaatggtt gtgacatgca gttgatgacc ggcacgcgcc
    21241 agattaatgg ccatcggtgt acccataatg cctaagccaa taaatcccag tttcataatt
    21301 aacctctttt aaatttcgct tttcctggat tcagacaaca cgatcccctg ccaacttagc
    21361 ggcctaaaaa agcattgcct gaacagcgtt acgttttaac ggtacggatc catccagcgt
    21421 aaaccggctt ccgtggtggt ttggggttta tattcacaac caacccaacc gttgtagtca
    21481 gaattttcga ttaccttaaa gagataatca taattaattt ctccggtccc cggttcgccg
    21541 cgatgcggat tatcggcaat ttgcaggtga ccaattttat cggcccactg agtcatggtg
    21601 ttggttaatt caccttccat ccgctgcatg tgataaatgt catactgaat tttcaaattg
    21661 cagcaaccaa catcatcaat caatttcagc gcctgccgag ttccggtgag atggaaacca
    21721 ggaatatcaa aatggttaat aggttcaatc agtaataaaa tatcttcttt catcagcata
    21781 ttcgcggcat aacgcaggtt ttctacaagc gttgcgtgaa tctgttcact gctgaaacca
    21841 gccggcgttt taccgaccag acagttaatt tttttattac ccagcgcacg ggcataacga
    21901 atcgctgctg ctacgccatc ccgaaactct tcttcacggc caggaataca ggcaataccg
    21961 cgctcccccg ccgcccagtc acccgccggt aaattgtgca gcgtatgttc gagtttatta
    22021 cttgccagca cctgttttaa ttcttcaatg tcgtagtcat aaggaaacat aaattcaacg
    22081 ccgcgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata ttcaccaaat
    22141 aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc atagtgcatg
    22201 aagcaggttt cagtcggtgc atccgctgcg ttatcggcga tatcttcaaa ttccatgacg
    22261 ttatccagtt cgctgcccat cgaaatattt gtcacacgct cgagaataac ttccacgact
    22321 accggtaccc gatattgcgc cattaaggct ttcgcctgtt caaaggccgg tgcaatatct
    22381 tccggtttga agacgcgaat cgctttacaa cctaaacctt ccgctacttt tacgtggtcg
    22441 acgccgtaac cgttcacttc gctggagttg atattctcga aagcgagttg cacgcagtag
    22501 tccatgtcaa aggcgcgttg tgactgacga atcaggccga gataggcgtt attcaccagc
    22561 acatggatgt acggaatgtt gaactgcgcg ccaacggcta actcttcaat caggaactgg
    22621 aagtcgaagt cgccagaaat cgccaccaca ttgcgtttcg gatcagcggc acaaaccccc
    22681 agcgccgccg gaatggtcca gcccagcggg cctgcctgcc cacagttgat ccagtggcgg
    22741 tctttaaaga catgcagcat ttgtgccgca gcgatttgtg acagaccaat ggtggtgaca
    22801 taacaaacat cgcgaccaaa ggctttgttc atctcttcat acacgcgctg cggtttcacc
    22861 ggcacgttgt cgaagtgggt tttgcgcagc aaagtgcgtt tgcgctgctg gcagtcggcg
    22921 acccattctt tacgacacgg cagacgaccc gctttttgca tctcctgcgc cacttcaacc
    22981 agcagtgtca gcgccgcttt agcatcagag acaatgccga gatccggaca cagcacgcgg
    23041 ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt gtatttctct
    23101 accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat gtcggacgcc
    23161 agcaacgttg cgttaccgta acgatgcgcg gtttgcagac cgaccatccc ggccatcagt
    23221 tcatgatcgt ccgggataca gccccagccc atcagcgttg ggatcaccgg aacgctggtc
    23281 agttcagcaa actgttgtaa cagtgcagct gcgtcagcat tgattacccc gcccccggca
    23341 acaatcaccg gacgttcggc ctggattaac atttctacag ctttttcgat ctgcatacgg
    23401 ctggcagcag gtttgtagac cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc
    23461 gcaacctgaa cgtcgaacgg taaatccacc agtaccggac ccggacgacc agaacgcatc
    23521 aggtgaaatg cctgttgcag cacgcgaggc accagcgccg cttcacgtac tgtaaccgcc
    23581 attttgctga ccggtttagc aattgcttca atatctacgg cctgaaaatc ttctttatgc
    23641 agacgggcgc gcggagcctg gccggtaatg cacagaatag gaatggaatc agcagaagca
    23701 gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg aagtccccag acatacgccg
    23761 atattccctg ccgctgcgcg ggtataacct tccgccatgt gcgaagcacc ttccacatga
    23821 cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa cggattgatt
    23881 gcagctcccg gaacgccgaa ggcggtggtg ataccttctt tctccagcac atacattgcc
    23941 gcgtcaacgg ctctcatttt tgccatttta ttcctacctc tattaattgg aaaaattttc
    24001 caactttcaa attttgtcaa aaaaacccgc tccgcagagc gggatgcgac agacactatg
    24061 gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg accagctcac
    24121 cctgactgac aaaacggtct tctgtcagtc ttgatgaagg cccggagatg gagatagcgg
    24181 caacaacact acctacatca tcgtaaattg ctgaagctat gcaattcaga cctacaacat
    24241 gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc ttcagcaagg
    24301 tgggcatatc cacaagcgta gttggcgtaa actgctgcaa accggtttgc agaatgatgc
    24361 tcatcaactc ctcttccgcc agcggataaa gcagcgcttt gcccgcaccg gaagcatgca
    24421 gtggcagacg actgcccagt ggcgcacaca tcctgaccat cgatttacac tctaactgac
    24481 caattaatac cgcttcattg ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta
    24541 acatcaggcg gcgcataaac ggcccggcga cggagaggac atcgcggtta tggatgtacg
    24601 ctgcaccgac gttaaagaca cctaatccta tatgccacca gcctaactga ctgtcctgat
    24661 agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat
    24721 ccagattgag agaaatatcg ctaaccgacg aacttccccc acttttttcc agatattgca
    24781 gaatcgcaat tccccgctct aatgcctgtg cgcccttctg tgccacaggc tccgcctgtc
    24841 ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc tacaagtgag
    24901 cataaacaat gaacaattag tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca
    24961 gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc gatcgatggt
    25021 cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt tacgatggta
    25081 attcactccc tgttcgccgt tggtaataaa agcccgcagg gttgacaggt ctggtttgtc
    25141 gtcacctaac gccacgacca ccacaaacac ctcacctttc atcgggataa aggcctgagt
    25201 acccagcgga tgacgttcga gttcgtgaat ggtcagcggc agattcgccg gttgcgcgcg
    25261 gttaatgctg ataagcgtac ggtcttgctc aagaatctca accagcgcca aatcgtggta
    25321 acgctccacc aggccattgt taatatggaa aaaatcccgt tgctgcgttt cgattacgtc
    25381 gccataagca ctaaaggctt cctgacttaa cggtaatacc tgaagtttca tctttttgtc
    25441 cttgtaacat aattccgttc aaccaccgcc gaccggtcag cttggtttca ataacaggca
    25501 atctaaaacc ttaagagaac aacatcaata caaaagttgg aatatttttc caaaactaga
    25561 tttagtccac aaattgaaca gggttatcat gtaaaaatac tgataaatca gaggataaga
    25621 aaataacgca acttgcggat ttaaaagggg atacctgaag atgttcgatc cagaaacctt
    25681 gcggactttc attgcggttg ctgaaacagg aagtttttca aaagcggcag aacgattatg
    25741 taaaaccacg gcgacgatca gttatcgcat taaacttctg gaagagaata ccggagtagc
    25801 gctgtttttc cgtacgactc gcagcgtgac gttgacagcg gctggcgagc atctactttc
    25861 ccaggccaga gactggctga gctggctgga aagtatgcca agcgagctgc aacaggtgaa
    25921 tgatggcgtg gaacgccagg tgaatattgt catcaacaac ctgctctaca ccccccaggc
    25981 cgtcgcccag ttgctggcgt ggctgaatga acgttacccc tttacccagt ttcacatctc
    26041 ccgacaaatc tatatgggcg tctgggactc gctattgtac gaaggttttg cgctggctat
    26101 cggcgtcacg ggaactgagg cgctggcaaa tacctttagt cttgatccct taggatcggt
    26161 gcaatggcga tttgtcatgg cggcggatca tccgctggcg aacgttgaag agccgctaac
    26221 agaagcgcag ttgcggcgct ttccggcggt caatattgaa gacagcgccc gcaccttaac
    26281 caaacgcgtc gcctggcgat tgccagggca aaaagagatt attgttcccg atatggaaac
    26341 gaaaatcgcc gcccatctgg cgggcgttgg cattggtttt ttgccaaaat cactttgcca
    26401 gtcaatgatc gataatcaac aactggtcag ccgggtaatc ccaacgatgc gccctccttc
    26461 gccattgagt ctggcatggc gcaaatttgg cagcggcaaa gcggtagaag atattgtgac
    26521 cttgtttacc cagcgcaggc cggaaatcag cggattttta gaaattttcg gcaacccacg
    26581 cagttaaaaa tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga
    26641 ggttcacttt taactatgca agagagacac acggaacagg actatcgtgc cctgctgatt
    26701 gctgatacgc ccattattga tgttcgcgcc cctatcgagt ttgagcaagg cgcaatgccc
    26761 gccgctatca atctgccgtt aatgaataac gatgaacgcg ccgccgttgg cacctgctat
    26821 aaacagcaag ggtcagacgc ggcgctggca ttgggacata aactggtggc gggtgaaatt
    26881 cgtcagcagc gcatggacgc ctggcgggca gcgtgcctgc aaaatccgca aggtattctc
    26941 tgctgcgccc gtggcggtca gcgctcacat attgtgcaaa gctggttgca tgcagcgggg
    27001 attgattatc cgctggtgga aggcggttat aaggcactgc gccagaccgc gattcaggcg
    27061 actattgaac tggcacaaaa accgatagtg ctgattggcg gttgtaccgg cagcggtaaa
    27121 acgctgttag tgcagcaaca gccgaacggt gttgatctgg aagggctggc gcgtcatcgc
    27181 ggttcggcgt ttggtcgcac attacaacca caacttagcc aggcgagttt tgaaaacctg
    27241 ctggctgccg aaatgctaaa aaccgacgcc cgtcagaatt tgcgcctgtg ggtgctggaa
    27301 gacgaaagcc ggatgatcgg ttcgaatcac ctgccggaat gcctgcgcga gcgaatgact
    27361 caggcggcga ttgcggtggt agaagatccg tttgagatcc gtcttgagcg cctgaacgaa
    27421 gagtatttct tgcgtatgca tcatgatttt acccacgcgt acggcgacga acagggctgg
    27481 caagcgtatt gcgaatacct gcatcacgga ctttcggcta ttaagcgtcg gctggggcta
    27541 cagcgctata acgaactggc tgcaaggctg gatgcagcgc tgacaacgca actcaccacc
    27601 ggcagcaccg acggtcatct ggcctggctg gtgccgttac ttgaagaata ttacgacccg
    27661 atgtatcgct atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg
    27721 gaagtggcgg aatgggttaa ggcgcagtga tgagggacgt atttgtatta gtcgcgactt
    27781 gatctgacac atggccttga aaggttgaga gttaccggtt ttgatatgag tgtcgaatcc
    27841 ttatccaaaa caagaggtaa ctctcatgct tcatactgcc aatcccgtca tcaaacacaa
    27901 agccggtttg ctcaatctgg ctgaagaact cagcaacgta tcaaaagcct gtaaaatcat
    27961 gggcgtttcg cgtgatacgt tttatcgtta ccgcgaactg gtcgctgaag gcggtgtgga
    28021 tgcgcagatt aatcgtagtc gccgcgctcc taaccttaag aaccgtaccg atgaggcaac
    28081 tgaacaggct gttgttgatt acgccgtcgc tttcccgaca catggtcagc accgggccag
    28141 caacgagctg cgtaaacagg gggtttttat ctccgacagt ggtgtccgtt ctgtctggtt
    28201 actccataac cttgagaatc tcaaaagacg atactgaaca aattttatca ggtgacgttc
    28261 cgtaaaaagt tgtatggagt tctcgatgca gtcggcgcga ctatcagtac ccccgctgaa
    28321 ccctggacta tcgggccgcc gtactggggc atgtcaccct ggcgtgccgc tggttttccg
    28381 ctaatggttt atttcctctt tctttcccgg caagaattta ttttaatccg gaaggattat
    28441 ttattttcag atagggactc acattaaaca cgtaacattt taattaatgc atggctgaat
    28501 atgtattaca caatgtcaga tatcgtaaaa gaccagtgca attctatgta aactctctga
    28561 ctgttcattt tatttgttgc ttcagggtcg gaactcaccc tcaataaaca actgaatacg
    28621 agtagatatt catcgtctga gctatatggc tttacacaat agccgacatg tcttttctca
    28681 gtcaggaatt ttacttttcc tcactacgaa gttgacgact tcgatatggg atagactctt
    28741 aattcaagca attactctgc acaagcatca ggcaatccag ctccaatatt tgttataaaa
    28801 taatgcgtaa cattaatttc accaacaaaa aaaaggttta cgctatcccc ttgccctggt
    28861 attttaccat atttacacca cacttttttt gtatctggat cattctctga tacgattttc
    28921 gccacttgtt ccctggccct caccgtcaat gttttcttaa acctcccccc atcatcgatt
    28981 gttagggaaa aataagaaaa aaaataatca tcttctacat ttgtggatat tgatatatcc
    29041 accgctacgt gcggaaaaaa atctgggagc catgtaaaaa ctccatcact ttctg
//
LOCUS       DAZFFG010000055        28939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFFG010000055 DAZFFG010000000
VERSION     DAZFFG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.055"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..417
                     /locus_tag="ACHJ7J_002666"
     CDS             <1..417
                     /locus_tag="ACHJ7J_002666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000882.1"
                     /translation="ESGETEMSVTVINEMLGRTFKEVFKNENDELVFRGDYSRGVWGT
                     VFTFFHFQECCEKVWIEDIVGDLDDLQGEPLTEAEVVTEECDVGECHQTWSFFKFGTS
                     KGCVTVRWCGESLGFYSERVSLKRELTDVFDFDSDF"
     gene            474..1190
                     /locus_tag="ACHJ7J_002667"
     CDS             474..1190
                     /locus_tag="ACHJ7J_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO5000883.1"
                     /translation="MENKSKYIAKIKKLLRLARGTSSPEEAANAIAKAQAYMRQHGIS
                     ENDAELFDIQEAASAGAPSDASTPPLYMHILCDLVCKAFGVECYISGEYRASGLLKRY
                     VRFYGPDSRPEIAAYAFDVLSRQMMAERKKYQDKHCKRCGPSTRVARGDQFCEGWVFG
                     ARDVIAVYDVSPEEKSRLELYRKNLHSTKGLRDGDMRTAKACRGAEFAATAGFIAGKN
                     ARLHQGVNGQSNRPLALGRM"
     gene            1193..1420
                     /locus_tag="ACHJ7J_002668"
     CDS             1193..1420
                     /locus_tag="ACHJ7J_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000884.1"
                     /translation="MAFKLLSVIEAIYQPPGERHEYRMNDGSAAVEFPKYPEASRWRF
                     YDSVGRRIIKRTVHNAMKAAVERHKRRFNCK"
     gene            1417..1647
                     /locus_tag="ACHJ7J_002669"
     CDS             1417..1647
                     /locus_tag="ACHJ7J_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000885.1"
                     /translation="MNIEFYNYGTKAKIIVTCWLWEFRRYNRVVDAALFVAPEVHHQS
                     GGGLLMRTVITGKTVPMLRAFKVAKQEAGNGH"
     gene            1637..1951
                     /locus_tag="ACHJ7J_002670"
     CDS             1637..1951
                     /locus_tag="ACHJ7J_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO5000886.1"
                     /translation="MVIDKTITIDVAMNTGLALIGYGYITFMSFRWLISSFFKQCEKR
                     RRKDKRVKALNAFNDAFDIDCMQQGDPARVITQGDIVILVYRNEKNEQEETSESANQN
                     KR"
     gene            1923..2087
                     /locus_tag="ACHJ7J_002671"
     CDS             1923..2087
                     /locus_tag="ACHJ7J_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000887.1"
                     /translation="MSQQTRISDKSLTRLIADADRVLERNTPVADEEWWQDFRAAMLE
                     LQERRREELK"
     gene            2084..2353
                     /locus_tag="ACHJ7J_002672"
     CDS             2084..2353
                     /locus_tag="ACHJ7J_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000888.1"
                     /translation="MSELKCRINGHQIEPCAVLAKALEYGNPTFKSKGIFIPERVNIN
                     TGEPGLDIAQIHSGQYVGRGVAMCFCPFCGESLKTWKKEATSERD"
     gene            2340..2795
                     /locus_tag="ACHJ7J_002673"
     CDS             2340..2795
                     /locus_tag="ACHJ7J_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5000889.1"
                     /translation="MSEINYQALREVAEAAKRDTTWQNLQAFHAAATPEVVLALLDVL
                     DASLEAEPVAWTDEEELRDVRKYGFGEIFQCPPDKYADPRRVISLYREPPAVPAVLER
                     LRAIVSDPRALPRRKEWVSGQQYSYVLLENVEAMVDEACRTAMLQGKGE"
     gene            2797..3162
                     /locus_tag="ACHJ7J_002674"
     CDS             2797..3162
                     /locus_tag="ACHJ7J_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5000890.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            3159..3491
                     /locus_tag="ACHJ7J_002675"
     CDS             3159..3491
                     /locus_tag="ACHJ7J_002675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000891.1"
                     /translation="MTTFTNSKLTDEYVSNATLIRLILWADQHNSHYVVAALRELQER
                     RKADSVPVEINDDMAYAFHHALSDSSLGSDEIEEIKTGLRAALANVTAQPAPVVPNGS
                     TVLIKRVD"
     gene            3555..3833
                     /locus_tag="ACHJ7J_002676"
     CDS             3555..3833
                     /locus_tag="ACHJ7J_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000892.1"
                     /translation="MKIEQIGMTAFRQQAESCGVDEFVVQRFGGVYHLFAVNRRAGVS
                     YFLQERRGDYKTWLSLDRAAAFLSGIGVSRFTVRFEDNKHAEKDDRGH"
     gene            3805..4200
                     /locus_tag="ACHJ7J_002677"
     CDS             3805..4200
                     /locus_tag="ACHJ7J_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO5000893.1"
                     /translation="MLKKMIGAIKAGQAYLGWDDALYRQTLARLTGKTSTTRCNLDEL
                     RIIREYMHEQGFPRKAPAGKGRRPRVAMSRKSVLSKIEALLADAGRSWAYAEGLASHM
                     YKQHVIEWLTDEQLFGVMVALVKDSRRRK"
     gene            4197..4535
                     /locus_tag="ACHJ7J_002678"
     CDS             4197..4535
                     /locus_tag="ACHJ7J_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000894.1"
                     /translation="MKKLLLLILTLCFTGISHASDSEEDLIKKTLQGDYQAQRNLAYS
                     YMNGWDDISKDTIKGCALRKVILLTQAQADIGDYGNEAIDCRKVHPTDNQKVWEYVRG
                     YLVLINENKK"
     gene            4635..5102
                     /locus_tag="ACHJ7J_002679"
     CDS             4635..5102
                     /locus_tag="ACHJ7J_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO5000895.1"
                     /translation="MQTFSETDLRDAQALLPDSVQQLISVIGFPALTRLIRSFGGVTL
                     SGKTGAHAERTGGVHALLHDVLTEDEINKLIRFLGGAPFYIPRCDHALRALRNTRFMA
                     DLQRHLRDGFSHRQALAQLCPRYGISDRYAWQLIHRRQKQTSLKSPTQVGLFD"
     gene            5128..5406
                     /locus_tag="ACHJ7J_002680"
     CDS             5128..5406
                     /locus_tag="ACHJ7J_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000896.1"
                     /translation="MTNYTALGEYTAYSEQARDAAGRRFAYMKNLANQLNRMAEQPDM
                     VVQEEALQCAIADIIASENEMRAAMEKANASAPLCNKPLITPDSLSRF"
     gene            5589..5981
                     /locus_tag="ACHJ7J_002681"
     CDS             5589..5981
                     /locus_tag="ACHJ7J_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5000897.1"
                     /translation="MSEPLSGGGAVAVTIGGASVFGLLTNTDFGVVVGAFAGALFVVT
                     QQKEIPVWRMAIHLLVAFVVGVLGAGVAASLMQWLTHYNDKPLDALCAVGVSALSIKI
                     LTFLYQQEISSLFGLFSRLRGGGGGNGK"
     gene            5971..6267
                     /locus_tag="ACHJ7J_002682"
     CDS             5971..6267
                     /locus_tag="ACHJ7J_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5000898.1"
                     /translation="MESSLAGIVNVWLCLAIVLGLFVYRRHGAAHKPMITWLAYWLML
                     GYVIIPFRWLSGTYTHSSWLVVALNLVFCALIVWAHGNLSKILSLLRHPHEIKR"
     gene            6251..6796
                     /locus_tag="ACHJ7J_002683"
     CDS             6251..6796
                     /locus_tag="ACHJ7J_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HFO5000899.1"
                     /translation="MKSKDKIFDEVLGKEGGYVNHPDDKGGPTKWGITEKVARAHGYR
                     GDMRDLTREQALDILEADYWYGPRFDQIAALSPDIAAELCDTGVNMGPTVASRMLQRW
                     LNVFNLRGRLYPDMDADGRIGPRTISALRAFLNNRGQDGERVMLTALNCTQGDRYLEL
                     AEKREANESFVYGWLKERVAV"
     gene            6793..6975
                     /locus_tag="ACHJ7J_002684"
     CDS             6793..6975
                     /locus_tag="ACHJ7J_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000900.1"
                     /translation="MTRQHWTHRTPRKAARCVLALFLVMLLPVGCTDINRAGQLFDAA
                     AQVCRIIDGIRQCSQN"
     gene            7049..7201
                     /locus_tag="ACHJ7J_002685"
     CDS             7049..7201
                     /locus_tag="ACHJ7J_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000901.1"
                     /translation="MVLAERITHFYRQYGTHDGTVIELDTGKNVFILENPDEVQRRIE
                     QAQKDT"
     gene            7201..7701
                     /locus_tag="ACHJ7J_002686"
     CDS             7201..7701
                     /locus_tag="ACHJ7J_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003569893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFO5000902.1"
                     /translation="MAWSQDIRDKVRNGYIFDQLPLDIVAMKYAVPHDTARRWKTQAM
                     KNGDDWDKLRAAHALAGDGLESVARTVLISLVVKCQTTLERLNQNPDIPPQESVELLA
                     SLSDSLSKAVASSKKILPETDRLATALEVVQRLGAFIKERYPAQYAPFLEVLEGFAKE
                     LEDNFS"
     gene            7748..8488
                     /locus_tag="ACHJ7J_002687"
     CDS             7748..8488
                     /locus_tag="ACHJ7J_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5000903.1"
                     /translation="MQKQIIGNATLYCGDVLDVLPALSERFDAVITDPPYSSGGTHKS
                     DRSMAPSDKYVGHTQYAEFSGDNRDQRSWAYWSTLWMSGAARLVRPGGYVMVFSDWRQ
                     LPTMTDVFQAGGVLWRGLVVWDKTQASRAPHTGYFRHQAEYVVWGSNGKLDKCPHGGP
                     FPGVITQRVVPSEKLHMTAKPVQLMAELVKPLAPDAHVLDPFMGSGTTAIPVLARGGR
                     FTGIEMTNQYFDIACARLEKAQAEAAQV"
     gene            8490..10130
                     /gene="terL"
                     /locus_tag="ACHJ7J_002688"
     CDS             8490..10130
                     /gene="terL"
                     /locus_tag="ACHJ7J_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFO5000904.1"
                     /translation="MAKRKLSIKEFQRSLQEYIANLRQTIEAECLGFDVNPQATQARR
                     AAVCDPVTGYDYFVENYFPHYVRNPAKSELHKYLFSRLPQVVASPDPENDAIAAPRGE
                     AKSTLVTQLFTLWNIIRAIKHYPVIIMDSIDQAYPMLEAIKAELEFNPRLKNDFPEVC
                     GQGRVWRMGTIVTANNIKVTVAGSGKKLRGLRHGPYRPDLVILDDIENDEMVRNPEQR
                     DKLHNWLTKTVMPLGEAGGKTDIIYIGTILHYDSVLSRTLNNPMWKTARFKAVIQWPA
                     NMKLWDEWEELIRNKQPEAAEALYRQNEADMLAGSVVSWAARPLLALMKIRVRDGHDT
                     FDSEYQNDPVSGEDALFAGCIKFWVNRLDEWVFYGAVDPSLGKKNKNRDPSAILVGGF
                     NRFTGILDVVEADIRRRLPNKIIEDVIKYQREYHCLCWSFESVQFQEFLRTVLVERSA
                     ALGVPVPALPVIPLEDKALRIESLQPHMANGLIRISPAHQTLIDQLRHYPKADHDDGP
                     DCLHMLWTLAVSRSAKFQIHTPRSTGRDRGGRFGSGGW"
     gene            10134..11729
                     /locus_tag="ACHJ7J_002689"
     CDS             10134..11729
                     /locus_tag="ACHJ7J_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFO5000905.1"
                     /translation="MAQLVDIYGRPLKREALKTTQSVRVAERLRIYPDHPSRGLNIRK
                     LPRILEAAERGYLPAQAMLFADMEERDGHLFAEMEKRKKALLTLDWSVEPPRNASKAE
                     KELAAAVDDWLHGIPDMEDIILNGMSSVGYGFSCQEISWAFVDKTWLPDAVTLRPHNW
                     FITLPEHNDELRLDDGNRGEDGKDGSALWPFGWLVHRYNARSGFLGSSGLFRVLVWPY
                     LFKNFALRDMAEFLEIYGLPARIAYYAQGTSDEDRDNILEALVNLGHEAVAALPQGNE
                     IEFKEAASGGPEAFMSMVEWAERTTSKVILGSTLTSQADGKTSTNALGNVHNEVRHDI
                     LTADARQLSGMFSSLIQMMASLNGWQDIPSRRLPRLVFDVQQEADIKGVAEAVSVLLN
                     NVGMKDIPASWVRKKTGIPTPKDGEEVLVPVAQPLPVQVGLSQLRERMNVAALSQQGN
                     GEDDPAQNAIDRAELPAEAIAQGMNELVAPLVQAIQEGRDADEAMNVLAEAWPELPDE
                     TLRQLLTQAFFVADIWGRLNADS"
     gene            11716..12881
                     /locus_tag="ACHJ7J_002690"
                     /pseudo
     CDS             11716..12881
                     /locus_tag="ACHJ7J_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141630.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
     gene            12878..13429
                     /locus_tag="ACHJ7J_002691"
     CDS             12878..13429
                     /locus_tag="ACHJ7J_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5000906.1"
                     /translation="MSSGKLDIKIDLSAYNTTLGKLIRSVKDRRDLMTALAGSMLDAV
                     ETNFEQQGRPKWMGWSPAYAKRRGPGQILQKSGRLAASIRSAVNNNEATVGTNVRYAR
                     IHNEGGEIRHQARTQNLYFKQYKNGSVSTRFVKKRNSNFVQSATVGAYTVNMPARPFL
                     QLVQDDIDELENTANRYFARVID"
     gene            13639..14754
                     /locus_tag="ACHJ7J_002692"
     CDS             13639..14754
                     /locus_tag="ACHJ7J_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO5000907.1"
                     /translation="MKMNIAALSLEITKATHSEIQLFPAGEFSAVDGRPHTDEVESGK
                     WVLTAELAAQLVAQVAARTTPFVIDYEHQTLRAVNNGKPAPAAGWFSQVEWREGAGLY
                     ATGVEWTENAAAMIAAGEYKFISPVFAYNKRGEVLELLHAALTNTPALDGMDAVMLAA
                     ASRLASLSIETETKTVNPEQLANLLAQLRWLLTLPETSTAEEVCAELQKIIDAVSGGE
                     GTAAASVNLLDLLNQKDEQIASLSANAYDPTKHIPLTAYEELQGRYAVLAQQSGEAEA
                     GALIQAALSDGRLLPAQEGWAKDYASRDINGFKSWLDNAPKLVALSQTQTGGKPPKTP
                     SPAPAQIKTGDDVDVDIAICSMMGVDPEDVARYAGDE"
     gene            14755..15150
                     /locus_tag="ACHJ7J_002693"
     CDS             14755..15150
                     /locus_tag="ACHJ7J_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000908.1"
                     /translation="MDRNTPYRDGELNPVPVAAATEIFGGHMVAVNASGYAVPASATA
                     SQITLGVSDGWADNSTGSDGDATVLVRCGKDFLMVNSTSDPVTQSQVGKLCYVEDSVT
                     VAKTDNSSARPVAGKVIGICGDGVWVHFS"
     gene            15161..16069
                     /locus_tag="ACHJ7J_002694"
     CDS             15161..16069
                     /locus_tag="ACHJ7J_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO5000909.1"
                     /translation="MLVNVKNVKQIFINLKATFQKAFDQSPTDWQKVAMEVPSNGKEN
                     DYSWLSRFPKMREWIGDKVVKSLAAFNYTIRNKDWEATVEVDRNDIEDDQIMGYALQA
                     KGAGQSAAELPADIVASLISNGFTNPCYDGQMFFDTDHLVAGKSVSNKGTKKLKVGSL
                     AEAKASYGAARTAMRSLKDDEGASLKIRPNLLVVPPALEDDANYLMTAEKFPDGTPNP
                     YRSTADVLVMPELASDSAWFLFDTTKPVKPLIYQLRKKPVFVEQTDYNSDNVFKRKKF
                     LFGAEARCNGGYGFWQMAFGSDGTTE"
     gene            16073..16414
                     /locus_tag="ACHJ7J_002695"
     CDS             16073..16414
                     /locus_tag="ACHJ7J_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO5000910.1"
                     /translation="MEKVIEITARREGFRRCGVAHSATTKAWPVDAFTPEQLAVLKAD
                     PMLIVVERDKASGQNDAVRGDELAAQLDAERQKVSELTAQLEEERQKVSELTAQLNAA
                     QKTQKAEKKEK"
     gene            16418..16849
                     /locus_tag="ACHJ7J_002696"
     CDS             16418..16849
                     /locus_tag="ACHJ7J_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp36 family protein"
                     /protein_id="HFO5000911.1"
                     /translation="MSYATPEQFIRAFSEREACTLTDEEMTGLINEEKLASALARASA
                     QIDGYLVGRYRTPWPDSPGVLVGYCCDIARYHLATDYRICSEEIQMRYRDAIRFLEKV
                     AAGQINLGRDTSGSVIPSSSQVRIRSGSRQFGRESTRGGAF"
     gene            16846..17484
                     /locus_tag="ACHJ7J_002697"
     CDS             16846..17484
                     /locus_tag="ACHJ7J_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO5000912.1"
                     /translation="MITEIERALVERLRCGLGKMVREVRTYAGELDEDPGRIVRSLPA
                     AWVTFGGIAKTERYSTSRQKYIATGRFVVVVGDYNTRSEQSARQGGTVRDEVGTNLLV
                     ESVRRLLTGQDLGLEIDYFEPGRVRTLFNTGVAERAMSVFACEFDTRWVEHALENGKW
                     PERGAEADMAFNRYHGRLSDPDPDLLRIGTQYGADICGLDELREQQHEQNEG"
     gene            17465..17698
                     /locus_tag="ACHJ7J_002698"
     CDS             17465..17698
                     /locus_tag="ACHJ7J_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO5000913.1"
                     /translation="MNKMKVKAAPGMKFPMEDNARKYITTEAVIVENTAYYRRAVQDG
                     DLILVAEEPETAEQDTGPEKAKVKKEKQVGSDE"
     gene            17691..19112
                     /locus_tag="ACHJ7J_002699"
     CDS             17691..19112
                     /locus_tag="ACHJ7J_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HFO5000914.1"
                     /translation="MSEIQFDTISGSIRKPGVHFEFNTRLAVNTLPGNEQRVLVIGPM
                     LPDGTATPLNAVSVYSEDEADLYFGAGSLAAAMARAAINANSYLQLDVIGIADGGTGK
                     AATGAVKVTGTATSSGTLSVWIAGEQIAINVDVGDEPSKIIPALVTAMTQTPSLLVTG
                     EYKSDTSQLTVTARTKGTWGNDITLSVSTTATGLVVATTPMASGEMDPDIQPALDAVF
                     AAGHNILICPFSTTPALAALKQHLEKTGNAMEQRGAIGCAGWTGSLGNGITLAAGVNS
                     GRVSVPWYRGSVMLPAVLAAIYGAVMASEEDPARPLNSLALSGLDVVAMSQRESRNEQ
                     ESALHNGLTPVEVGPGNTVQIVRAVSTYTVNAQGVADVSLLDITSIRTLDYTRKACRE
                     RISLRFPREKLSTRTIAKVESELYDVLLKLEESEILENVEANKAKLRVQRNGKDANRL
                     DCAIPADVVNGLHVFAGRIDMIL"
     gene            19125..19499
                     /locus_tag="ACHJ7J_002700"
     CDS             19125..19499
                     /locus_tag="ACHJ7J_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5000915.1"
                     /translation="MSLKEYIGSIVLEIDSQEIEITDLDVQINTGRKLVKTMNKSGRA
                     KGFARGIATYELSVSAVIPDTNEPDWENLEGVKISLYPLSNSGKRTSYLDCFTTEVGE
                     KYTVDNEAKIDIKMAALRKVEG"
     gene            19496..19879
                     /locus_tag="ACHJ7J_002701"
     CDS             19496..19879
                     /locus_tag="ACHJ7J_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000916.1"
                     /translation="MTIIATQTGELSDGLVFNGTIHKNFELRLPVMRDNGQALEETEE
                     RFQTVDGFAADYYYRCAVMAATLVRLGDIPQEELTAELLHDNMTPEDFNILLASRNVL
                     KVKRSGGNPGSPDSGSQSLSSGATE"
     gene            19894..20052
                     /locus_tag="ACHJ7J_002702"
     CDS             19894..20052
                     /locus_tag="ACHJ7J_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000917.1"
                     /translation="MTMSRPELDGWLAAVDRLNGGGSGRKDTSQTTRQSFKSLRKKRR
                     KGKQKNGR"
     gene            20042..22315
                     /locus_tag="ACHJ7J_002703"
     CDS             20042..22315
                     /locus_tag="ACHJ7J_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5000918.1"
                     /translation="MAGNFKVGMTLTAKDEASQVLEKGQKQVIKATEGVTKATRKAGA
                     EQTRTGQESVNSTKKAAKEIQRAARARETLGIRAEREIRREIYQTVASYNRLARAGFE
                     SAQEQERAMQATREKARALKRELDGVTQAQMKMAKTPVIPERGRFARAAAFGGNAVTI
                     GGGIAAGAAIMAQPVKKQMSYERQLAMMANTAFSDGGLEGRKAGLEQMKSSIRNAVTY
                     GGGTKEDAAETLDALLKDGGISFDTASKWLPELMKYATASGASATDLANVMLKGKKTF
                     GFRDEDISTVLNMAIAAGKEGSFELNDMARWLSQQMGSASAAGMKGKDDFVKILALNE
                     AAAITAGSSDEAGNNVVNLLAKLTSHDIETAAKSIDYNGKGIDFSGTLTEARKHGLDP
                     IDALSSLIDKIVANDKRYQELQKKLSSARDKGEQTAVYDSMAKLLEGFGVGKLVADRQ
                     ALMALLAYRNNPEYRKKVEDAINQQRTLPEGQRAGDLDFKFMSGTNDFKTEQAKNTLE
                     FSQMDSVKKLADASGTVADAISWAGEKFPGLTTAVVGATTAIEAMTAAALTWAGIKIL
                     TGGKPGGKTGEVVGDVIENTVKKGKGFKFPGIAGGLLSLGGTVTALATATSPEEDAAV
                     EGSEERWKRIRARYPQEIIDAARKKYQPWWQFGEGYSTENEEWLRRWEEDRKKAASDA
                     LPSPAQVNKAAGNTTPEPASTQPGRITQPEYLTHWGPPASPINFTTQLVLDGQVVAEA
                     VNKYNLQDGNRGTGGTY"
     gene            22315..23664
                     /locus_tag="ACHJ7J_002704"
     CDS             22315..23664
                     /locus_tag="ACHJ7J_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO5000919.1"
                     /translation="MGWAENLQNASFRGVQFEVLNTDEQISRDHAVYEYPFVDGADLH
                     DLGRKARPFRMTAFLWGDDYEYKLGKLTAALDEGGDGELIHPVYGSVPSVIVTGYSIR
                     HDAESPDSCTIDMSFLENRTGSALFSTPLPELFAQQLFDELDTLQARLSDFFDAVTAP
                     LNTVNSMIKKAQTVRATLVNTLLSFKSDFVSTVDNIVSLAGEPGKFINELAEVLEIHT
                     SDVGQPVQVLQRVDSATRTGLAGETTVASSATVMNCWNKVITDMDEFVALPVTLVSGD
                     KVAEVALPVDASPDDVQDVKAVYATQAASELASVAMAILVDDAQSEQLIPADIGRLVG
                     DVRTRLQAAITLFRERYEGERERITETASPLGLMYPEIIQSMKNVAATVQDVGLLVLS
                     RRPPLTQKQVQADSCLLLLAWQWYGDYSRAAELQRLNPQLRDPNNITAGVVINAYAK"
     gene            23648..24859
                     /locus_tag="ACHJ7J_002705"
     CDS             23648..24859
                     /locus_tag="ACHJ7J_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO5000920.1"
                     /translation="MPTPNNEDKISLVIAGRSHSDWSSYRIDSDFLKAADGWQLQLGL
                     PEKVFPADIVRGAQIRLQVGDETVLSGRIDSVRRSVSRQSCTLALSGRDDAAILVDCA
                     APVFSANQLTLDEVIDRIVRPLGIQRIRIQASGVSRNDKVVIEPGMRAWDALAKAAAG
                     RGLWPWFEPDGTLVVGGPDYTTTPVDTLILKLNGTGNNVMELDDTRSINGCFSELTVL
                     AQSHARRADSKKQVAVVPLDIWNEDGSVRTLSGQDSGNTDSGQTGIHNMKAVATDPTV
                     DYYRPQIITQGDTSNLEQVNWRAKKMMSDARLSGLDIVALVAGHRTADGVLWQPGQRV
                     RIVSELHGIDAIFFLMGREFSGGRNGQTTRLRFKEDGVWIPDAFPREKKRHHRRGKKK
                     KEVAIVKVWEK"
     gene            24859..25509
                     /locus_tag="ACHJ7J_002706"
     CDS             24859..25509
                     /locus_tag="ACHJ7J_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5000921.1"
                     /translation="MWDKVNQRVQQALAAVRQAFRVVTGTVDSSTKVQLLQLNGLAGE
                     QLDGAEYFQHYGLTTSPPPGSMGIAVPLNGNTSHTVIVATEHGAYRLTELKPGEVALY
                     TDEGAKIVLKRGRVIETECDVYRVKCKRYEVEAEENATFTTPLLTASDRLTVEGKITG
                     NGGMAISGGKGYTATFEGNINHVGGVITSVDVTINGVKIGTHKHPTPHGMSDTPVN"
     gene            25563..25913
                     /locus_tag="ACHJ7J_002707"
     CDS             25563..25913
                     /locus_tag="ACHJ7J_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO5000922.1"
                     /translation="MDQTISPATGDYERRRIYTLHNAVYLRLATPLGSYWADASLGSR
                     LHELKREKDVSRVHKLAAQYASQALQPLLDDGRAKSITVDTKPGQRGWLLLLITVTDN
                     AGTPQTFEHPVRIM"
     gene            25913..26992
                     /locus_tag="ACHJ7J_002708"
     CDS             25913..26992
                     /locus_tag="ACHJ7J_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5000923.1"
                     /translation="MPFPVPGVAENTERQLRDIANALPGETIDTGADSDYRIRANAVS
                     GVADGLYMHQGWILRQVFPDTADPEYLELHCRTRNVFRKKATASSGPAVITGTPGKTL
                     PAGAEIRGEGVSVATAADCTVGDEGSAEVTVKSTATGAQTNASTTQTATLVSPPEGIN
                     STVTIKSLTGGTDRESDADLLARYLDILRRPPAGGNKYDYKRWALEVDGVTSAYVEPL
                     RRGLGTVDVAITSANDLPSQELINAVLAHIEEVRPVTAKDTMVLAPTKKAVDFVVRVK
                     TSGLTVEQIKPQITEVITDFMNRLEPGQELIISQLETQISLISGVSDRRIIMPSDNVK
                     AVVNESTWEWLRPGSIDIQPFPRES"
     gene            26996..27568
                     /locus_tag="ACHJ7J_002709"
     CDS             26996..27568
                     /locus_tag="ACHJ7J_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO5000924.1"
                     /translation="MNTVDLFRAMLPPVSYDPNGKYISAELNAEAAIMDEVKASAARV
                     LASVTPFYASMTLSDWERVYGVIPRDGATQQERRENILIKMAATGGLSIPYFKQLAAS
                     LGYTITITELHPFRAGINRCGDRLYITDARWIWQVNVTGSRTPAYRFRTGVSAAGEPL
                     LSFGAPVLEETFKDLKPAFTYCHFTYKEEQ"
     gene            27568..28371
                     /locus_tag="ACHJ7J_002710"
     CDS             27568..28371
                     /locus_tag="ACHJ7J_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5000925.1"
                     /translation="MQNLMPPINTPDQLFHDGDPTQGIEGTLVTAGYLNNQQGATRDL
                     QQELLNVLGGAHIQPDPKKTDQLLTALRALLLSRKNPFGDIKLDGTVQKALENLGLGE
                     GSALPVGVPVPWPSATPPTGWLKCNGAAFSAEKYPELAKAYPTNKLPDLRGEFIRGWD
                     DGRGMDTGRAILSAQGDAIRNIYGEFKTVNTENYSIWESAGSFKGAVVPLNPSTNNSY
                     FSLIRSMVTERTDGAVYPKVIGLDASRIVPTANENRPRNIAFNYIVRAA"
     gene            28371..>28939
                     /locus_tag="ACHJ7J_002711"
     CDS             28371..>28939
                     /locus_tag="ACHJ7J_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5000926.1"
                     /translation="MNKAVLNNELIAIKAGVITIYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPVTHKAGYAICRSADFNSWEYVPDHRGKIIYSTETGESKEITAPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQPENTRLNAVLDYIDAVTATDTSTAP"
BASE COUNT         7062 a         7319 c         8445 g         6113 t
ORIGIN      
        1 gaatctggag aaacagaaat gagcgttacc gttatcaatg aaatgcttgg gcgtacattt
       61 aaagaagtat ttaagaatga aaacgatgaa cttgtttttc gtggtgatta ttcccgggga
      121 gtatggggaa ccgtgtttac gtttttccac tttcaggaat gctgcgaaaa agtctggatt
      181 gaggacatcg tcggagacct tgacgacctg caaggcgagc cgctgacaga ggctgaagtg
      241 gttacagaag aatgtgatgt tggggaatgt catcagacat ggagcttctt taagttcggg
      301 acatcaaaag gctgtgtaac cgtcagatgg tgcggcgaaa gcctgggttt ttattccgaa
      361 cgtgtatcac tgaagcggga attaactgac gttttcgatt tcgattctga tttctgattt
      421 cgttttttat ggcgcaggta aacaggtctg cgcctgaata agagaaattc attatggaaa
      481 ataaaagcaa atacattgcc aaaattaaaa agctgttacg ccttgccaga ggaacatcaa
      541 gcccggaaga agccgctaac gctattgcaa aagcccaggc ttatatgcgc cagcatggca
      601 tcagcgaaaa cgacgcggaa ttgttcgaca ttcaggaagc tgccagcgcg ggtgcgccga
      661 gtgacgccag tacgccgccg ctttatatgc acatcctgtg tgacctggtc tgtaaagcct
      721 tcggtgtcga gtgttacatc tccggcgaat accgggcttc tggtttactc aaacgttacg
      781 tgcgttttta tggtcctgac agtcgcccgg aaatcgcggc ttacgcgttt gatgtgcttt
      841 cccgccagat gatggcagag cgcaagaaat atcaggataa acactgcaaa cgttgcggcc
      901 cgtccacgcg ggtagcccgt ggcgaccagt tctgtgaagg ctgggtcttt ggtgcccgtg
      961 atgtcattgc ggtgtatgac gtctccccgg aagaaaaaag ccgccttgag ctttacagaa
     1021 agaacctgca cagcactaag ggactgcgcg acggggatat gcgcacagct aaagcatgcc
     1081 ggggtgcaga gtttgccgct actgccggtt ttatcgccgg gaaaaacgcc agactgcatc
     1141 agggagttaa tggtcagagc aacagaccgc tggcactggg gaggatgtaa cgatggcttt
     1201 taaacttctc agtgttatcg aagcaatata ccagcctcct ggagaacgcc acgaatacag
     1261 gatgaatgac ggtagcgcgg cggttgagtt tcccaaatat ccggaggctt cccggtggcg
     1321 gttctatgac agtgtgggac gccggattat taaaagaacc gtgcacaacg ccatgaaagc
     1381 cgctgtagaa cgtcacaaaa ggaggtttaa ctgcaaatga atattgaatt ttataactat
     1441 gggactaaag caaaaatcat tgttacctgc tggctctggg agtttcgccg ctataaccgg
     1501 gttgtggatg cggcgctgtt tgtggcccct gaagtgcacc accagagtgg tggaggcctt
     1561 ttaatgagaa ccgtcatcac cggtaaaacg gttccgatgt tacgggcgtt taaagtggca
     1621 aaacaggagg ctggcaatgg tcattgataa aacgataacg atagatgtag ccatgaatac
     1681 aggtctggcg cttattggtt atggctatat cacttttatg tcattcaggt ggttgatttc
     1741 ttcgttcttt aagcaatgtg aaaaacgtcg ccgcaaagac aagcgcgtga aggcactaaa
     1801 tgcgtttaat gacgcttttg atattgactg tatgcaacag ggcgaccccg cacgcgtaat
     1861 tacccaggga gatatcgtaa ttcttgtgta ccggaatgaa aaaaatgaac aggaggaaac
     1921 cagtgagtca gcaaaccaga ataagcgata agagcctgac cagactgatt gccgatgcgg
     1981 acagggtgct ggagcggaac acccccgttg ccgatgagga atggtggcag gatttccggg
     2041 ccgcaatgct ggaacttcag gaacgtcgtc gggaggagtt gaagtgagtg aattaaagtg
     2101 tcgaattaat ggccatcaga tagaaccgtg tgcggtactg gcaaaagcac tcgaatatgg
     2161 aaatccaacc tttaagagta aaggcatatt tatcccggaa cgcgtgaata taaacaccgg
     2221 cgagccaggc ttagacattg ctcaaattca ctccggccaa tatgtcggtc gtggtgttgc
     2281 aatgtgtttt tgcccgttct gtggtgaaag tctgaaaaca tggaaaaagg aggcaaccag
     2341 tgagcgagat taactatcag gcactgcgtg aggtggcaga ggccgcgaag agggatacga
     2401 cgtggcagaa cctacaggct tttcatgctg cggctacgcc ggaggttgta ctggcattgc
     2461 ttgatgtatt ggacgcatcg ctggaagcag aaccggtggc atggactgat gaagaggaac
     2521 tgcgcgatgt caggaagtat ggtttcggtg agatatttca gtgtccgcca gataaatatg
     2581 cggacccgcg tcgtgtaatc tcgctgtatc gcgagccgcc agcagtacca gctgttctgg
     2641 aacgtttgcg agctattgta tctgacccac gcgcattacc tcgcagaaaa gaatgggtta
     2701 gtgggcagca gtacagttac gtacttctcg aaaacgtaga agctatggtt gatgaagcct
     2761 gccgcaccgc catgcttcag gggaaaggag agtgatatgg cactgacgaa aaaacaacgt
     2821 gcagagctgc gcatgaagtt cggcggtcgc tgtgcttatt gcggctgcga acttggcgaa
     2881 aagtggcatg cagaccatgt aaaaccggtc attcgttttg atggaaatat gcttcaccag
     2941 gaacgtgacg atatatccaa catggttccg gcatgccacc catgcaatct gcacaagcat
     3001 tgcagtagcc tggaagatta tcggcgaatt atcagtgatg gtcgtcgtga attccttgcg
     3061 tccgggaaag gcaaggcgct ggttcgtatg ggattggttg aaatgaaatc tgacccggtt
     3121 gtgttctggt ttgaaaaata tcaagaaggg gctacggcat gaccactttt accaatagca
     3181 aactaacaga cgaatacgtt tcaaatgcaa cgttgattcg gctcattctg tgggctgacc
     3241 agcacaatag ccattatgtt gtagcggctc tgcgcgagct acaggagcgc cgcaaggctg
     3301 atagtgtgcc tgtagaaatc aacgacgaca tggcttacgc attccaccat gcgctttctg
     3361 actcatcgct tggctctgat gaaatcgaag aaattaaaac cgggctgcgt gccgcccttg
     3421 ccaacgttac cgcccaacca gcaccggtag tgccaaatgg gagcacggtc ttaattaagc
     3481 gggttgatta aacagtaatc aagcgcagac caaaacatat cttttggtct gccaaaagtg
     3541 atgttttggt cattatgaag attgaacaaa tcggaatgac agctttcagg caacaggctg
     3601 aaagctgtgg cgtggatgag tttgtcgtgc agcggttcgg tggtgtgtat catctattcg
     3661 ctgtgaatcg ccgcgccggg gtgtcctatt ttctacagga acgccgtggc gattacaaga
     3721 catggctgtc tcttgaccgt gccgctgcgt tcttgtcagg gattggcgtt tcacgtttta
     3781 ccgtgcgttt tgaggataac aaacatgctg aaaaagatga tcggggccat taaagccggt
     3841 caggcgtatc tggggtggga tgatgccctt tatcgccaga cgctggcccg cctgaccggt
     3901 aaaaccagca ccacacgctg caatctcgat gaactgcgca tcatccggga atacatgcac
     3961 gaacagggat tcccgcgcaa ggcacccgct ggtaaaggtc gccgccctcg tgttgcaatg
     4021 agcagaaaat cagtattatc caaaattgaa gcgttgctgg ctgatgccgg tcgttcctgg
     4081 gcgtatgcag aaggactggc atctcacatg tataagcaac atgtgatcga atggctgacg
     4141 gatgaacagc tttttggagt gatggtggcg ctggttaaag attcaaggag acgtaaatga
     4201 aaaaactgtt attacttatt ctgactttat gttttacagg gatatcccat gcttctgatt
     4261 cagaagaaga tttgatcaaa aaaacattac agggcgatta tcaggcacaa cgtaatctgg
     4321 cttattcata catgaatggc tgggatgata tttcaaagga taccatcaaa ggatgcgctc
     4381 tcaggaaggt tattctctta acacaagccc aagctgacat tggcgattat ggaaatgaag
     4441 ccatagattg tcgaaaagtg catccaacag ataaccagaa agtatgggag tatgtcagag
     4501 gatacctggt gttgataaac gagaataaaa aataatatga acaaaaacct tcgcaaacgc
     4561 ggaggttttt tatggtctgt acaggcgatc atgtatggct ataataacag taagttacta
     4621 tcggagacgc catcatgcag accttcagtg aaacagatct tcgcgatgca caggcactgc
     4681 tacccgattc cgtgcagcaa ctgattagcg tgattggctt ccccgccctg acccggttaa
     4741 tccgttcttt tggtggcgtg acgttaagcg gtaaaaccgg cgcacacgcc gaacgcactg
     4801 gcggtgtcca tgccctgtta catgacgtgc tcaccgaaga cgaaatcaac aaactgatcc
     4861 gctttcttgg cggcgcaccg ttttacattc cccgttgcga tcatgcttta cgtgccctgc
     4921 gtaacacccg ttttatggct gatttacagc ggcatctcag ggacggattc tcacatcggc
     4981 aggcgctggc acagctttgt ccccgttacg ggatttcaga ccggtacgca tggcaactga
     5041 tacaccgacg acaaaaacag acttcgctga aaagccccac ccaggtgggg ctttttgatt
     5101 aatgggcctc agaaaaagga gacacttatg accaactata cggcacttgg cgagtataca
     5161 gcctactcag agcaggcccg ggatgctgcc ggacgccgct tcgcttatat gaaaaatctg
     5221 gccaaccaac taaaccgaat ggccgaacag ccggatatgg ttgttcagga agaagcgttg
     5281 cagtgtgcca ttgctgacat catcgccagc gaaaacgaga tgcgcgcagc gatggaaaag
     5341 gcgaatgcct ctgctccgct ttgtaataaa cctcttatta cgccggattc tctttcccgc
     5401 ttctgattta gctccccgcc gccgcggggt gctgaactcc cccagcagta cccaccacac
     5461 cagaccgttt accctgtcca ctcagaccac aaggcacctt aagggatagc gccctgcaaa
     5521 aggcttatgt gcctttgaca gggtgttatt tacgggccgt cagtggctca gatttacagg
     5581 agaaactcat gtctgaaccc ttatcaggcg gcggagcggt ggcagtcacc atcggtggtg
     5641 ccagtgtgtt tggccttctc accaataccg atttcggggt cgtggtgggc gcgtttgccg
     5701 gggcgctttt tgttgtcacg cagcagaaag aaatcccggt atggcgaatg gctatccacc
     5761 tgctcgtcgc gtttgtggtt ggcgttctgg gggctggcgt ggctgcgtca ctgatgcagt
     5821 ggctgaccca ctacaacgac aagccactcg atgcattgtg tgcggtcggt gtctcggcgt
     5881 tatccatcaa gatactgacc tttctttatc aacaggaaat ttcatcgctg ttcggcctgt
     5941 tttccagact gcgcggcgga gggggtggaa atggaaagta gccttgccgg tatcgtgaac
     6001 gtctggctgt gtctcgccat tgtgctgggg ttgtttgtgt atcgccgcca cggtgcagcg
     6061 cataaaccga tgattacctg gctggcgtac tggctgatgc tcggctacgt catcatcccg
     6121 ttccgctggc tgtccggtac gtacacacat tccagctggc tggttgtggc gctgaatctg
     6181 gttttctgtg cgcttatcgt gtgggcgcac gggaatttgt caaaaatcct ttcgttactg
     6241 aggcatccac atgaaatcaa aagataaaat ttttgatgaa gtcctgggca aagaaggtgg
     6301 ttatgtcaat cacccggatg ataagggcgg tccgaccaaa tggggcatta cggaaaaagt
     6361 cgcccgcgcc cacggatacc gtggcgatat gcgcgattta acccgcgaac aggcactgga
     6421 catccttgaa gccgattact ggtatggccc gcgatttgac cagatcgccg cattatcccc
     6481 tgatattgcc gcagagctgt gtgataccgg tgtgaatatg gggccaaccg tggcgtccag
     6541 aatgcttcag cgctggctga atgtgttcaa tctgcgcggc aggctgtacc cggatatgga
     6601 cgcagacgga cgcatcgggc ctcgtaccat cagtgcattg cgcgccttcc tgaataatcg
     6661 cggtcaggac ggtgaacggg tgatgctgac ggccctgaac tgtacgcagg gggaccgcta
     6721 tctggaactg gcggagaaac gcgaggctaa cgaatcgttt gtttatggct ggctgaagga
     6781 gcgcgtggcg gtatgacgcg tcagcactgg acccacagaa cgccacgcaa agccgcgcgc
     6841 tgtgttctgg ccctgtttct ggtgatgttg cttccggtgg gatgcacaga cattaacaga
     6901 gcgggccagc tgtttgacgc ggcggcgcag gtctgccgga ttattgacgg tatccggcag
     6961 tgttcgcaga actgattttt tgaggtctga catagtgagc attatcagat ttacagaata
     7021 cagaggttca ggaggtgctg gcgctgtcat ggtgctcgct gaacgcatca cccattttta
     7081 ccgtcagtac ggtacacatg atggcacggt cattgaactg gataccggga aaaacgtgtt
     7141 cattcttgaa aatcccgacg aagttcagcg caggattgaa caggctcaga aggacactta
     7201 atggcgtggt cacaggatat cagggacaaa gtccgtaacg ggtacatctt tgaccagctt
     7261 ccgctggata tcgtcgccat gaagtacgcc gtgccgcacg acaccgcgcg gcgctggaaa
     7321 acgcaggcga tgaaaaacgg cgacgactgg gacaagctgc gtgccgctca cgcgctcgcc
     7381 ggtgacgggc tggaaagtgt cgcccgcacc gtgcttatca gtctggtggt gaagtgtcag
     7441 acgacgcttg aacggctgaa tcagaacccg gacatcccgc cgcaggagtc cgtcgaactg
     7501 ctggcgagtc tgtctgacag tctcagtaag gcggtggcca gcagtaaaaa aatcctgccg
     7561 gaaaccgatc ggctcgccac ggcactggaa gtggtgcagc ggctcggcgc gttcatcaag
     7621 gagcgttacc cggcacagta tgccccgttt cttgaggtgc tcgaaggctt cgctaaagag
     7681 cttgaggata atttcagtta accacagcgg cccggttttc cgggccgtct gatattcggg
     7741 atttgtaatg caaaaacaga ttattggtaa cgcaacgttg tattgcggtg atgttcttga
     7801 tgtgctgcca gcgttatctg aacgttttga tgccgtcatt accgacccgc cttacagcag
     7861 tggtggcaca cacaaatcag accgcagcat ggccccttcg gacaagtatg tcggtcacac
     7921 ccagtatgcc gagttcagcg gggataaccg cgaccagcgc agctgggcat actggagcac
     7981 cctgtggatg tccggggctg cccgccttgt gcgccccggt ggttatgtca tggtgttcag
     8041 tgactggcga cagcttccca ccatgaccga cgtttttcag gccggtggtg tgctgtggcg
     8101 tggtcttgtt gtctgggata aaacgcaggc ttcccgtgcg ccacacaccg gctatttccg
     8161 gcatcaggcc gagtatgtgg tctggggcag caacggaaaa ctcgacaaat gcccgcacgg
     8221 tggcccgttc ccgggtgtga ttacgcagcg tgttgtcccg tctgaaaaac tgcacatgac
     8281 cgcaaagccg gttcagctga tggccgagct ggttaaaccg ctggcaccgg atgcgcacgt
     8341 cctcgatccg tttatgggaa gcggcaccac cgccatccct gtacttgccc gtggcggacg
     8401 gtttaccggc attgaaatga ctaaccagta ttttgatatc gcctgcgcac gtctcgaaaa
     8461 agcgcaggca gaagcagcgc aggtgtgacg tggcaaaacg taaactttcc attaaagagt
     8521 ttcagagaag cctgcaggag tacatcgcca acctccgcca gaccattgag gctgaatgtc
     8581 tggggttcga tgttaaccca caggccacac aggcccgccg ggcggcagtc tgcgatccgg
     8641 tgacgggcta tgattatttt gtcgaaaatt atttcccgca ctacgtccgc aacccggcaa
     8701 aaagcgagct gcataaatac ctgttcagcc gtctgccaca ggttgtggca agcccggacc
     8761 cggagaacga cgccatcgcc gcgccccgtg gtgaagcaaa atccacgctg gtgacgcagc
     8821 tgtttaccct gtggaacatt atccgggcca ttaaacatta cccggtcatc attatggaca
     8881 gtatcgacca ggcttatccg atgctggaag ccatcaaggc tgaactggaa tttaaccccc
     8941 gtttaaaaaa cgatttcccg gaggtgtgcg gacaggggcg cgtctggcgt atggggacca
     9001 tcgtcacggc caacaatatc aaggtgaccg ttgccggtag tggtaaaaaa ctgcgtggtc
     9061 tgcgccacgg tccgtaccgt cctgacctgg tcatcctcga cgacatcgag aacgacgaga
     9121 tggtgcgtaa cccggagcag cgcgacaagc tgcataactg gctcaccaaa accgtgatgc
     9181 cgctgggcga ggctggcggt aaaaccgata ttatttatat cgggaccatc ctgcattacg
     9241 actctgtgct gtcgcgcacg ctgaataacc cgatgtggaa aacggcccgc tttaaggctg
     9301 ttattcagtg gcctgccaat atgaagctgt gggacgagtg ggaagaactc atccgcaata
     9361 aacagccgga agcggcggag gcactttacc ggcagaacga agccgacatg ctcgccgggt
     9421 cagtggtgtc atgggcggcg cgtcccctgc tggcgctgat gaaaatccgt gtccgtgacg
     9481 gtcacgacac ctttgattct gaataccaga acgacccggt cagcggtgaa gatgcgctgt
     9541 ttgcgggttg cattaagttc tgggttaatc gtcttgatga atgggtgttt tatggtgcgg
     9601 ttgaccccag tctcgggaag aagaataaaa accgcgaccc gtcagccatt ctcgtggggg
     9661 gctttaaccg ctttaccggc attctggatg tggttgaagc cgatatccgc cgacgtctgc
     9721 cgaataaaat tatcgaagac gtgatcaagt atcagcggga ataccactgc ctgtgctggt
     9781 cgtttgagtc cgtccagttt caggagtttc tgcgtaccgt gctggttgag cgttcggcgg
     9841 cgctgggtgt gccggttccg gcgctgccgg tcatcccgct ggaggataag gcgctgcgta
     9901 tcgaatccct tcagccgcat atggccaacg gactgattcg catcagtccg gcacatcaga
     9961 cgctgattga ccagctgcgc cactacccca aagccgacca cgatgatggc ccggactgtc
    10021 tgcacatgct gtggacgctg gcggtatcgc gtagcgcaaa atttcagatt cacaccccac
    10081 gcagtaccgg gcgtgaccgt ggcgggcgtt ttggttcagg aggatggtaa ttaatggcac
    10141 agcttgttga tatttacgga cgcccgctga aacgtgaggc tctcaaaacc acgcagtcgg
    10201 tcagagtggc ggaacggctg cgcatttatc ccgaccaccc gtcccgtggt ctgaacatca
    10261 gaaaactgcc gcgcattctg gaagctgccg aacgcggtta tcttccggcg caggcgatgc
    10321 tgtttgcgga tatggaagaa cgtgatggcc atctgttcgc cgagatggaa aagcgcaaaa
    10381 aagcattgct gacgctggac tggtcggtgg agccgccccg caatgcctcg aaggcagaaa
    10441 aggagctggc cgccgccgtg gatgactggc tgcacggtat cccggacatg gaggacatca
    10501 tcctcaacgg gatgagcagt gtcggttacg gtttcagttg tcaggaaatc agctgggcgt
    10561 tcgtggataa aacgtggtta ccggatgcgg tgacgctgcg accgcataac tggtttatta
    10621 ccctgccgga acacaatgac gaactccggc tggatgacgg caatcgtggg gaagatggta
    10681 aagacggttc cgcgctgtgg ccgttcggct ggctggttca tcgttataac gcccggtcgg
    10741 gatttctggg gtcatccggc ctgttccgcg tactggtctg gccgtatctg tttaaaaact
    10801 ttgcgttgcg tgatatggcg gaatttctgg agatttacgg ccttccggcg cgcattgcct
    10861 actatgcaca gggcaccagt gacgaagacc gggataacat tcttgaggcg ctagtcaatc
    10921 tggggcatga agcggtggca gccctgccgc agggtaacga gattgagttc aaggaagccg
    10981 catccggcgg accggaggcg ttcatgtcga tggtggaatg ggcggagcgt accacctcaa
    11041 aagtgattct gggcagtacg ctgaccagcc aggccgacgg taaaacatcc accaacgcac
    11101 tgggtaatgt ccataacgag gtccggcacg acattctgac cgctgatgca cgccagctgt
    11161 ccggcatgtt cagcagtctg atacagatga tggccagtct caacggctgg caggatatcc
    11221 cgtcacgccg tcttccgcgc ctggtgtttg atgtgcagca ggaagctgac attaagggcg
    11281 tggcggaagc cgtcagcgtg ttgctcaaca atgtggggat gaaggatatc ccggcgtcgt
    11341 gggtgcgcaa aaaaaccggc attcccaccc cgaaagacgg cgaagaggta ctggtgccgg
    11401 tggcacagcc tctccctgtg caggttggcc tcagccagct gcgtgagcgg atgaatgttg
    11461 ccgcactcag tcagcagggc aacggggagg acgacccggc acagaacgcc atcgaccggg
    11521 cagagcttcc ggcagaggcc atcgcgcagg ggatgaacga actggtggct ccactggtgc
    11581 aggccataca ggaaggccgg gatgcagacg aggctatgaa cgtactggcg gaagcgtggc
    11641 cggagctgcc tgatgaaacg ctgcggcagt tgctgacaca ggcattcttt gtggcggata
    11701 tctgggggcg actgaatgcc gacagctgac gatgttgacc tgggttatgc gtacaccctg
    11761 aagccggaag aagcgattaa gtatttcgaa agcaagggat acgttatcgg cttccgctgg
    11821 cacgatgtga aggacatcgc caacgcccgg gcgttcacgg tggcgggcgt gctgaaactg
    11881 gatgtgctga aggatatccg tgatggcctg acggccgcac tggctgacgg cgggacgttc
    11941 cgggagtttg ctgcacaact ggaaccactg ctggaatcaa aaggctggct gggtaaaaag
    12001 ctgattgtgg acgaggacac tggcgaactg cacggcaggc agctgacgcc gcgccggttg
    12061 cgcacgatat ttgataccaa tattcagtcg tcctacaacg ccggacgcta tcagcagcag
    12121 atggcgaacg tggccgaccg gccttatttt gagcgcgtgg cggtgatgga ccttcacacc
    12181 cgcccgaaac acgccgccct gaacggtttt accgcccgag cggatgaccc ggtctgggag
    12241 tatttctacg cgccggacgg atacggatgc cgctgccgta tccgggcgcg ttcggcgtct
    12301 gatgtggaga aatatggctt gacggtgcag agcagcgagg ggcgactggt tgaggttgag
    12361 caggaatatg gccagccggg ccagaccatc agaacaatgg ggctgaagat gccggatggc
    12421 tccgtataca ccgctgaccc gggcttcggt tttaatcccg gtcgggtggc atggcagccg
    12481 gagctggaaa aatatgatta ccgcagcgcc cgccagtacg tcaccggcac cctgaccggg
    12541 ccggactttg cccgggggct ggcgaacgtc agcgaactgg atgcgcgcca gcggtatcca
    12601 ctggccatcc gttcgccgga acaggtcgcc gccacgggcg ctgcacggca gacggtaaac
    12661 cttacggctg acgtcatgaa gcgcctcagt gcaacagaca cgccccgacc gccgccgact
    12721 atgtgctgat gcagcagacc atcgaacggg cggagcatgt cacacaggac ggcaacgcgt
    12781 ggcggtatgc gttacagtcg ggcgaccact ggtcggtggt gacggttgag gatgacgcgc
    12841 tgacagaatg ggttatgcag gataccccgg aggcatcatg agcagcggta aactggatat
    12901 taagattgat ttaagcgcgt ataacaccac gctggggaaa ctgattcgtt ccgtgaagga
    12961 ccggcgcgac ctgatgacgg cacttgcagg ctccatgctg gatgcggttg agactaactt
    13021 tgaacaacag ggtcgcccga aatggatggg ctggagtccg gcttatgcga agcggcgcgg
    13081 ccccgggcaa atcctccaga agtccgggcg actggctgcc agtatccgct ctgcggttaa
    13141 caataacgag gccacggtcg ggacaaacgt ccgttacgct cgtatccaca acgaaggcgg
    13201 cgaaatccgc catcaggcac ggacgcagaa cctgtatttt aaacagtaca aaaacggcag
    13261 cgtcagcacc cgctttgtga aaaagcgcaa cagtaatttt gtacagagcg caacggtcgg
    13321 agcgtatacg gtcaacatgc ccgcgcgccc cttccttcag cttgtgcagg acgacatcga
    13381 cgagctggag aacaccgcaa accgctattt tgcgcgtgtg attgactgaa tgaccagaaa
    13441 cgcgctgtaa tcacctctga cgacataaac gcctcacgga tgaggcattg cagcaccgca
    13501 ccccataaat gccgtcagaa tcgtttttaa aagggtttta aaaacggttt tatttcccct
    13561 tttatccttt cccgttatgg cagggtgctg aacaccctcc acagtacccc gtgttcccct
    13621 tccgtcatca tgccgcgtat gaagatgaac attgcggcgt taagcctcga aattactaaa
    13681 gcaacccaca gcgaaatcca gctgtttccg gcgggcgagt tcagcgcggt ggatggtcgc
    13741 ccacataccg atgaagtcga aagcggtaaa tgggtgctga ccgctgaact ggccgcgcag
    13801 cttgtcgccc aggtggcagc ccgtaccacg ccatttgtca ttgattacga acatcagacg
    13861 ctgcgcgccg taaacaacgg caagcccgcc ccggcggcgg gctggttcag ccaggtggaa
    13921 tggcgagaag gtgcgggcct gtatgccact ggtgtggagt ggacggagaa cgcggcggcc
    13981 atgattgccg ccggtgagta caaatttatt tcccctgttt ttgcctacaa caagcgcggc
    14041 gaagtgctgg agctgttaca tgctgcgctg accaataccc cggcgctcga cggtatggac
    14101 gcggtcatgc tggctgcggc cagccgtctg gcgagtctgt caattgaaac ggagaccaaa
    14161 accgtgaacc cggaacaatt agcaaattta ctcgcgcagt tacgctggct gctgacactg
    14221 ccggaaacct caaccgcaga ggaagtctgc gcagagctgc aaaaaattat tgatgctgtc
    14281 tctggtggcg aaggaacggc ggcagcgtct gtcaatctgc tggacctgct gaaccagaaa
    14341 gacgagcaaa tcgccagcct gtcagcaaat gcctatgacc cgacgaagca tattcctctg
    14401 acggcatatg aagaacttca ggggcgttat gccgtactgg cgcaacagtc cggtgaggcc
    14461 gaagccgggg cactgattca ggcggcgctg tctgacgggc gactgcttcc ggcacaggaa
    14521 ggctgggcga aagattacgc cagccgcgat atcaacggct ttaagtcctg gctggataac
    14581 gcaccaaaac ttgttgcgct cagtcagacc cagaccggcg gcaaaccacc caaaacgccg
    14641 tcgcctgccc cggcgcagat taaaaccggc gatgacgtcg atgtcgatat cgccatttgt
    14701 tcaatgatgg gcgtcgatcc agaggatgtc gcccgttacg caggagatga gtaaatggat
    14761 cgcaataccc cctatcgtga cggcgaactg aacccggtgc cggttgccgc tgcgaccgaa
    14821 atttttggcg gtcatatggt ggccgttaac gcatccggtt atgccgttcc ggccagtgcc
    14881 acggcctcac agattacgct gggcgtgtct gatggctggg cggataacag taccggcagc
    14941 gatggcgacg ccaccgttct ggtgcgttgc ggcaaagatt ttctgatggt gaacagcacc
    15001 tcagacccgg tcacacaatc gcaggtcggc aagctgtgct acgtggaaga cagcgtaacc
    15061 gtggcaaaaa ccgacaacag cagcgcccgt cctgtcgccg ggaaagtgat cggcatctgt
    15121 ggcgatggcg tctgggttca tttcagttaa ggagcaaaac gtgttagtca acgttaaaaa
    15181 cgtcaaacag atttttatca atctgaaggc caccttccag aaagccttcg accagtcgcc
    15241 aactgactgg cagaaggtgg caatggaagt accatcaaac ggcaaggaaa acgactacag
    15301 ctggttaagt cgtttcccga aaatgcgcga gtggattggc gacaaggtcg ttaaatcact
    15361 ggcggcattt aactacacca tccgtaacaa ggactgggaa gccacggttg aagtcgatcg
    15421 taacgacatc gaggacgacc agattatggg ctatgccctt caggcaaaag gagccggaca
    15481 gtcggcggca gaactcccgg cagatattgt ggcgtccctt atcagtaacg gttttaccaa
    15541 tccctgttac gacggccaga tgttctttga taccgaccac ctggtcgccg gtaaatcggt
    15601 gtccaataaa ggcaccaaaa aactcaaagt tggctcgctt gccgaggcga aagcctccta
    15661 cggtgccgcc cgtactgcca tgcgcagcct gaaagatgac gaaggcgcat ccctcaaaat
    15721 tcgcccgaat ctgctggttg tgccaccggc gctggaagat gacgcgaact acctgatgac
    15781 cgccgagaag ttcccggacg gtacgccgaa cccgtaccgc agtaccgccg acgtgctggt
    15841 gatgccggag ctggcgtcgg attctgcgtg gttcctgttt gacaccacca aaccggtgaa
    15901 accgctgatt tatcagctgc gtaaaaagcc tgtttttgtg gagcagacgg actacaacag
    15961 cgacaacgtg ttcaagcgta agaagttcct gtttggtgcc gaagcgcgct gtaacggcgg
    16021 ttacggcttc tggcagatgg catttggttc tgatggtacg acggagtaat gcatggaaaa
    16081 ggtgattgaa attaccgccc gccgcgaggg ttttcgccgc tgcggtgtgg cacacagcgc
    16141 aaccacgaag gcatggcctg tggatgcgtt caccccggaa cagctggcgg tgctgaaggc
    16201 tgaccccatg ctgattgtgg tggagcgcga taaagcgtcc ggccagaacg acgcggtccg
    16261 gggtgatgaa ctggccgcac agctggatgc cgaacgtcag aaagtcagcg aactgacggc
    16321 gcagctggaa gaagaacgcc agaaagtcag tgaactgacc gcacagctga acgccgcaca
    16381 gaaaacacaa aaagcggaga aaaaggagaa gtaacccatg tcttacgcca caccggaaca
    16441 gttcatcagg gcgttcagtg aacgcgaggc gtgcacactg acggatgaag agatgaccgg
    16501 gcttatcaac gaggaaaagc tggcctccgc gcttgcgcgt gccagtgccc agattgatgg
    16561 ctatctggtg gggcgttacc ggaccccgtg gcctgacagc ccgggggttc tggtgggtta
    16621 ctgctgcgat atcgcccgtt atcacctggc gaccgattac cgtatctgct cagaagaaat
    16681 tcagatgcgc taccgggacg ccatccgctt tctggagaaa gtcgcggcag gacaaatcaa
    16741 cctcgggcgg gatacgtccg gcagcgtgat cccgtcatcg tcacaggtgc gtatccgctc
    16801 cggctcccgt cagttcgggc gtgagtccac gcggggaggt gcattctgat tactgaaatt
    16861 gaacgggcgc tggttgaacg tctgcgttgt ggtctgggga aaatggtgcg ggaagtccgc
    16921 acctatgccg gtgaactgga tgaagacccg ggccggattg ttcgcagcct tccggcagcc
    16981 tgggtgacgt tcggcggcat cgcgaaaacc gaacgctatt ccacgtcacg ccagaaatac
    17041 attgccaccg gacgctttgt ggttgtggtg ggtgattaca acacccgcag cgaacagagc
    17101 gccagacagg gaggcaccgt ccgggatgag gtcggtacaa acctgctggt tgaatccgtc
    17161 cgtcgcctgc tgaccggtca ggatttgggg ctggagattg attatttcga acccgggcgt
    17221 gtcaggacgc tgtttaacac cggcgtggca gagcgggcaa tgtccgtgtt tgcctgtgag
    17281 tttgataccc gctgggtgga gcacgcgctg gagaacggca aatggccgga gcgcggtgca
    17341 gaagcggata tggctttcaa ccgttaccac ggccgtctgt cagaccccga cccggattta
    17401 ctgcgaattg gtacgcaata cggcgcagat atctgtggtc tggatgaatt aagagagcaa
    17461 cagcatgaac aaaatgaagg ttaaggcggc tcccgggatg aagttcccga tggaggataa
    17521 cgcccggaaa tacatcacca cggaagcggt gatcgttgaa aacaccgctt attaccggcg
    17581 cgctgttcag gatggtgacc tgattctggt ggctgaagag cctgaaacgg cagaacagga
    17641 caccggacca gaaaaagcga aggtgaagaa agagaaacag gtgggctccg atgagtgaaa
    17701 ttcagtttga caccatttcg ggcagtatcc gtaagcccgg tgtgcatttt gagtttaata
    17761 cccggctggc cgttaacacg ttgccgggta acgaacagcg tgttctggtg attggcccga
    17821 tgctgcctga tggcaccgcc acccccctga atgcggtttc tgtgtattcc gaagacgaag
    17881 cggatttgta tttcggggcc ggttcactgg ccgcagcaat ggcgcgcgcg gccattaacg
    17941 ccaacagcta tctgcaactg gatgttatcg gtattgcaga cggtggtaca ggaaaagcag
    18001 caacaggcgc agttaaagtc accggcactg caaccagcag cggtacgctg tcggtatgga
    18061 ttgctggcga gcagatcgcc attaatgtgg acgtcgggga tgaaccgtcg aaaatcattc
    18121 cggcactggt gacggcaatg acgcagacgc catcgcttct ggtcacgggg gaatacaaat
    18181 ctgacacctc tcaactgaca gtgaccgccc ggaccaaagg tacctggggg aatgatatca
    18241 ccctgtcggt atccaccact gcaaccggcc ttgtagtggc taccacgccg atggcgagcg
    18301 gcgaaatgga cccggatatt cagcccgcac tggatgcggt ctttgctgcc ggtcacaaca
    18361 ttctgatttg tccgttcagt accacaccag cccttgccgc cctgaagcag cacctggaga
    18421 aaaccgggaa cgcgatggaa cagcgtggcg cgattggttg tgcgggctgg acaggtagtc
    18481 tcggaaacgg gatcactctg gcagccggtg tgaacagcgg gcgcgtgtct gtcccctggt
    18541 atcgtggttc tgtgatgctt cctgccgtgc tggccgccat ctacggcgca gtaatggcga
    18601 gcgaagaaga cccggcacgc ccgctgaact cgctggcgct gtccgggctg gatgtggtcg
    18661 ccatgtcaca gcgcgaaagc cgtaacgagc aggaaagcgc cctgcataac ggcctgacac
    18721 cggttgaggt tggaccgggt aatacggtgc agattgtgcg tgcggtcagt acctacacgg
    18781 tgaacgctca gggtgtggct gatgtctcgt tactggatat cacgtccatc agaacactgg
    18841 actacacccg caaggcgtgc cgcgaacgta tcagccttcg cttcccgcgt gaaaaactca
    18901 gcacccgaac catcgcaaaa gtggaaagcg aactgtatga cgtgctgctc aagctggagg
    18961 agtctgaaat tctggagaat gtggaagcga ataaggcaaa actgcgcgtt cagcgaaacg
    19021 ggaaagatgc aaatcgcttg gactgcgcga tccctgccga cgtggttaac ggcctgcatg
    19081 tgtttgccgg tcgcatcgac atgattttgt aaggagcctg ataaatgtcc ctgaaagaat
    19141 atatcggctc gattgtgttg gaaattgaca gccaggaaat cgagatcacc gaccttgatg
    19201 ttcagatcaa caccgggcga aagctggtca agaccatgaa caaaagcggc agggctaaag
    19261 gctttgcccg tggcattgcc acctacgaac tgtccgtttc ggcggttatt cctgatacca
    19321 acgagccgga ctgggaaaat ctggaaggtg tgaaaatctc gctgtacccg ctcagtaaca
    19381 gcggcaaacg cacctcttat ctggactgct tcacaaccga agtgggtgag aaatacaccg
    19441 tcgataatga agcgaaaatt gatatcaaaa tggctgcact caggaaggtg gaaggatgac
    19501 gattatcgct acacagaccg gcgaactgtc cgatggtctg gtttttaacg gcaccattca
    19561 taaaaacttt gagctgcgtc tgccggtcat gcgtgataac gggcaggcac tggaagaaac
    19621 tgaagaacgc tttcagacag tggacggctt cgcggcggat tattactacc gctgtgcggt
    19681 aatggcggca acgctggtcc gtctggggga tattccgcag gaagaactga cggcggagct
    19741 gttgcacgac aacatgacgc cggaagattt taatattctg ctggcatcac gcaacgtcct
    19801 gaaggtaaag cggagcggcg ggaatcccgg ctcgccggac tccggctcgc agtccttatc
    19861 ctcgggcgct acggaataac cgaagaacaa atcatgacca tgagccgtcc cgagctggac
    19921 ggctggcttg ccgccgttga caggctgaac ggcggcggct caggaagaaa ggacacctca
    19981 cagaccaccc gccagtcatt taaatccctc agaaagaaac gcagaaaagg taaacagaaa
    20041 aatggccggt aatttcaaag ttggcatgac cctgactgca aaggacgaag cctcgcaggt
    20101 tctggaaaag ggacaaaaac aggttatcaa agccaccgaa ggtgtaacaa aggcaacccg
    20161 gaaggcaggc gcagagcaga cgcgtaccgg gcaggaaagc gttaacagca cgaaaaaggc
    20221 ggcaaaagag atacagcgtg ccgcccgtgc ccgggaaacg ctcggtattc gtgcagaacg
    20281 tgaaatccgg cgtgaaattt atcagacggt tgccagctat aaccgtctgg cccgcgccgg
    20341 ttttgaatct gcgcaggagc aggagcgcgc catgcaggcc acccgggaaa aagcccgggc
    20401 actcaagcgt gaactggatg gcgttactca ggcacagatg aagatggcga aaacgcctgt
    20461 tatccccgaa cgggggcgtt ttgcgcgtgc ggctgcattt ggcgggaatg ccgtgacaat
    20521 aggtggtggt atcgcggcag gtgctgccat tatggcgcag ccggtcaaaa agcagatgag
    20581 ctacgaacgc cagctggcaa tgatggccaa taccgccttc agtgatggcg ggctggaggg
    20641 gcgcaaagcc ggtcttgagc agatgaaaag cagcatccgt aacgcggtga cctatggtgg
    20701 cggcacaaaa gaggatgcag ccgaaacact ggatgccctg ctgaaagatg gcggtatttc
    20761 atttgacaca gccagcaagt ggctacctga actgatgaaa tacgccacgg cttctggtgc
    20821 atcagcgaca gacctggcta atgtgatgct taagggcaaa aagacctttg gattccggga
    20881 tgaagatatt tccactgtcc tgaatatggc gattgcggca ggaaaagaag gcagctttga
    20941 actgaacgat atggcccgct ggttatccca acaaatggga tctgcgtcag ctgcgggaat
    21001 gaaaggcaag gatgatttcg tcaagattct tgcgctgaat gaagccgccg cgatcacggc
    21061 tggtagcagt gatgaagcag ggaataacgt ggttaacctg ctggctaaac tgaccagcca
    21121 tgatattgaa actgcggcaa agagcattga ttacaacggt aaagggattg atttttcagg
    21181 tacgctgact gaagcgcgca agcatgggct ggatccgata gacgcgcttt ccagcctgat
    21241 agataaaatc gttgccaacg ataaacgcta tcaggagttg caaaagaaac tgtcatctgc
    21301 ccgggataaa ggtgaacaga cggctgttta cgattcgatg gcaaaactcc ttgaaggctt
    21361 tggtgtcggg aaactggttg cagaccgtca ggcgttgatg gcgttgttag cctaccgtaa
    21421 caacccggaa taccgtaaga aggttgaaga cgcgattaat cagcaacgca ccctgccgga
    21481 aggtcagcgc gccggagatc tggattttaa attcatgtca ggcacgaatg attttaaaac
    21541 cgaacaggct aaaaacacgc ttgaattttc acaaatggac agcgtgaaaa agctggctga
    21601 tgcgtccggt actgtggccg atgccataag ctgggcgggt gaaaagtttc cggggctgac
    21661 cacggcagtg gtgggtgcta cgaccgcaat tgaagcgatg accgcagcgg ctctgacatg
    21721 ggccgggatc aaaatcctga ccgggggtaa acccggtggc aagactggcg aggttgttgg
    21781 tgatgtaatt gaaaacacgg tcaaaaaagg caaaggattt aaattccccg gtattgccgg
    21841 tggcctgctt tctttaggtg gcactgttac ggcactggcc accgcaacca gcccggagga
    21901 agacgccgcc gtcgaaggaa gcgaggaacg ctggaagcgc atccgcgccc gatatcccca
    21961 ggaaatcatc gacgccgccc gcaaaaaata ccagccgtgg tggcagtttg gggaaggcta
    22021 ctccaccgaa aacgaagagt ggttgaggcg ctgggaggaa gacaggaaaa aagctgcatc
    22081 tgatgccctg ccatcaccgg cgcaggttaa caaagccgca ggaaacacaa cgcctgaacc
    22141 ggcatcaacg caacccggac gcatcaccca gccggaatac ctcacgcact gggggccacc
    22201 tgccagcccc attaatttca ccacacaact ggtgctcgat ggtcaggtcg tggcggaggc
    22261 agtgaacaaa tacaaccttc aggacggtaa cagaggtacg ggaggaactt actgatgggc
    22321 tgggctgaaa acctgcaaaa cgcttccttt cgtggcgtgc agtttgaggt gctgaatact
    22381 gacgaacaaa tcagccgcga ccatgcggtc tatgaatacc cgtttgttga cggggcggat
    22441 ttgcacgacc tcggacgcaa ggcgcgaccg ttccgcatga cggcgttcct gtggggcgat
    22501 gactatgaat ataaactcgg gaaactgacc gccgccctgg atgaaggcgg cgatggtgaa
    22561 ctgattcatc cggtttatgg ctctgtgccg tcggtgattg tgaccggcta cagcatccgc
    22621 catgacgcag aaagcccgga cagctgcacc attgacatga gttttctgga aaaccgcacc
    22681 ggtagcgcgt tgttcagcac cccgttaccg gagctgttcg cgcaacagtt gtttgatgaa
    22741 ctggacactt tacaggcgcg gttaagtgat ttttttgatg ccgttactgc acctttaaat
    22801 accgttaaca gcatgattaa aaaagcccag acagtgcgtg ccacactggt gaataccctg
    22861 ctgtcgttca aaagtgattt tgtctccact gtcgataata tcgtgtcact ggcaggcgaa
    22921 cccggaaaat ttatcaatga actggcagag gtgctggaaa tccacacatc agatgtcggg
    22981 caaccggtgc aggttctgca acgtgttgat tctgccaccc ggaccgggtt ggcaggagag
    23041 acgaccgtcg ccagttctgc gacagtgatg aactgctgga ataaggtcat cactgatatg
    23101 gatgaatttg ttgcgttgcc tgttaccctg gtcagtggcg ataaagtagc ggaggtggca
    23161 cttcctgtgg atgcctcgcc agacgatgtg caggatgtga aagcagttta cgcaacacag
    23221 gccgcatctg aactggccag tgttgcaatg gcgatactgg ttgatgatgc gcagtcagaa
    23281 cagcttatac cggcggatat cgggcgactg gtcggggatg tgcgcacccg cttacaggct
    23341 gccatcacgc tgttccgtga acgctatgaa ggcgaacgcg aacggataac cgaaaccgca
    23401 tcaccgctgg ggctgatgta cccggaaatc attcagagca tgaaaaacgt ggcggcaacc
    23461 gtgcaggatg tcggcctgct ggttctgtca cggcgcccac cgctgacgca gaaacaggtg
    23521 caggcggaca gctgtctgct gttgctggcg tggcagtggt acggcgatta cagtcgtgcg
    23581 gcagaactgc aacgcctgaa cccgcagtta cgtgacccga acaacataac cgccggggtg
    23641 gtaattaatg cctacgccaa ataacgaaga caaaatcagc ctggtcattg ccggaaggtc
    23701 gcattctgac tggagcagtt atcgtattga cagcgatttc ctgaaagctg ccgacggctg
    23761 gcagcttcag ctggggctgc ctgaaaaggt gttcccggcg gatatcgtcc ggggcgcaca
    23821 aatccggttg caggtgggag acgaaacggt gctcagtggg cgcatagaca gcgtgcgccg
    23881 cagtgtgtcc cgtcagagct gcacgctggc cctgtccggg cgtgatgatg ctgccattct
    23941 ggtggactgt gccgcgccgg tgttcagcgc caatcagctg acgctggacg aggtcatcga
    24001 caggatagtc agaccactgg ggatacagcg catccgtata caggcgtccg gtgtgtcacg
    24061 taatgacaaa gttgttattg agcctgggat gcgtgcgtgg gatgcactgg ccaaagctgc
    24121 cgccggtcgc ggcttatggc cgtggtttga gcctgacggg acgctggttg tgggtggccc
    24181 tgactacacc accacgccgg tggatacgct gattctgaag ctgaacggca ccgggaacaa
    24241 cgtgatggag ctggacgata cccggtccat taacggctgt ttctccgaac ttactgtgct
    24301 ggcccagagc cacgcccgcc gcgcagacag caagaaacag gtggccgtgg tgccgctgga
    24361 tatctggaac gaggacggca gcgtgcgcac attgtccggg caggacagcg gtaatacgga
    24421 cagtggccag acaggcattc ataacatgaa agccgttgcc accgacccca ccgtggatta
    24481 ttaccgcccg cagataatca cccagggcga caccagcaat ctggagcagg tgaactggcg
    24541 cgcaaaaaaa atgatgagtg atgcccgcct gtcggggctg gatatcgtcg cactggttgc
    24601 aggtcatcgc acggcggacg gtgttctgtg gcagcccggc cagcgagtac ggattgtcag
    24661 cgaactccac ggcattgatg ccatcttttt cctgatgggg cgtgagttca gcggaggccg
    24721 gaacgggcag acaacccggc ttcgctttaa ggaggacggc gtgtggatac ctgacgcctt
    24781 cccgcgcgag aagaaacgcc atcaccgcag gggcaaaaag aaaaaagagg tcgccattgt
    24841 taaggtctgg gagaaatgat gtgggacaag gttaatcagc gcgtacagca ggcactggcc
    24901 gccgttcgcc aggcattcag ggtggtgacc ggtacggtgg acagttcgac caaagtacag
    24961 ttgcttcagc tgaacgggct ggcaggcgaa cagctggatg gtgcggagta ttttcagcat
    25021 tacggcctca ccacatcccc gccgcccggc tcaatgggta ttgccgttcc gctgaatggc
    25081 aatacctccc ataccgtcat cgtggccaca gagcacggcg catatcgtct gacggaactg
    25141 aaacccgggg aagtggccct gtataccgat gaaggcgcga aaatcgtact gaagcgcgga
    25201 cgggttattg aaaccgaatg tgacgtttat cgcgtgaaat gcaaacgcta cgaggttgag
    25261 gcagaggaga acgccacatt cacgacaccg ttactgacag ccagtgacag gctgacggtg
    25321 gaaggcaaaa tcaccggcaa cggtggcatg gccatcagtg ggggcaaggg atacactgcc
    25381 acctttgaag gcaatatcaa ccatgtgggt ggagtgatta cctccgttga cgtcaccatt
    25441 aatggcgtta aaatcggaac gcacaaacat ccaaccccac acggcatgtc tgacacgccg
    25501 gttaattaag tgctgaacac cctcacctga ttctgacctg tccatgctgc cagactggcg
    25561 gcatggacca gacgatttca cctgcaaccg gcgactacga acgccgtcgg atttataccc
    25621 ttcataacgc ggtttatctg cgactggcga cgccgcttgg cagttactgg gcggatgcgt
    25681 cgctggggtc acgcctgcac gagctgaagc gggaaaaaga cgtttcccgt gttcataagc
    25741 ttgcggcgca gtatgccagc caggcacttc agcccctgct cgatgacggg cgggcaaaat
    25801 ccattaccgt tgacacaaaa ccggggcagc gcggctggtt gttgctgtta atcaccgtca
    25861 cggataacgc gggcacaccg cagacgtttg aacaccctgt gaggattatg taatgccgtt
    25921 tcctgttccg ggcgttgctg aaaacacaga acgccagcta cgtgatatcg ctaacgccct
    25981 gccgggcgaa accatcgaca ccggtgctga cagcgattac cgcattcgtg caaatgccgt
    26041 atccggcgtg gcggacggac tgtatatgca tcaggggtgg atactccgtc aggtgtttcc
    26101 tgatacggca gaccctgaat atctggagtt gcactgccgc acgcgcaatg tttttcgtaa
    26161 aaaagcaacg gcctcatccg gtccggcagt gattaccggc acacccggta agacgctgcc
    26221 agccggtgcg gaaattcgtg gtgaaggtgt cagcgtggcc accgcagcag actgcaccgt
    26281 cggtgatgaa ggcagcgcag aggtgacggt aaaaagcact gccacgggtg cacagacgaa
    26341 tgcatccacg acgcagacgg caacgctggt cagcccacct gaaggcatca acagcacggt
    26401 gacgattaaa tccctgaccg gcggaacgga cagggaaagc gacgccgacc tgctggcgcg
    26461 ttatctggat attctgcgca ggccgcccgc tggcggtaat aaatatgatt acaaacgctg
    26521 ggcgctggaa gtggatggcg ttacgtctgc gtatgtggag ccgttgcgtc gtggactggg
    26581 gacggttgat gtggccatta cgtcagccaa tgacctccct tcgcaggaac tgattaatgc
    26641 cgtgctggca catattgagg aagtccgccc ggtgacagcg aaggacacga tggtgctggc
    26701 cccgacgaaa aaagccgttg atttcgttgt ccgggtaaaa accagtggtc ttactgttga
    26761 acagataaaa ccacagataa ctgaagttat cacggatttt atgaaccggc tggagccggg
    26821 gcaggaatta attatttcac aactggaaac tcagatttca ttaatttctg gcgtcagtga
    26881 ccggcgaatt attatgccgt cagataatgt aaaagcagtt gtcaatgaat ccacatggga
    26941 gtggctgcgt ccggggagta ttgatatcca gccatttccc cgggagagtt aatacatgaa
    27001 tacggttgat ttatttcgtg ccatgttacc accggtcagc tatgacccga acgggaaata
    27061 tatttcagca gaactgaacg cagaagcagc tattatggat gaagtaaaag cgtcagctgc
    27121 acgagtgctt gcatctgtca caccatttta cgcatccatg acgctgtctg actgggaacg
    27181 agtgtatgga gtaatacccc gcgacggtgc cacacagcag gaacgccgag aaaatattct
    27241 tataaagatg gctgcaactg gtgggttatc tatcccttat tttaaacaac ttgcagccag
    27301 cctcgggtac accataacaa ttactgaact tcatccattc agagcgggga taaaccgatg
    27361 tggtgatcgt ctctatataa cagatgcccg atggatttgg caggttaatg tcacaggaag
    27421 cagaacgcca gcctacagat tcagaaccgg agtatctgct gccggggagc cgttactttc
    27481 ctttggtgcc cctgtactgg aagaaacatt taaagacctt aaacccgctt ttacttattg
    27541 ccatttcaca tataaggagg agcagtaatg caaaatctga tgcccccgat aaatacaccg
    27601 gatcaattgt ttcatgatgg tgaccccact caggggatag aaggaaccct cgtgactgct
    27661 ggttatttaa acaatcagca gggggcaacc cgtgatttac agcaggaact ccttaatgtt
    27721 cttggtggtg cacatattca acctgacccc aaaaaaacgg atcagctact taccgcgctt
    27781 cgcgcgttgc tgttaagccg caagaatccg tttggcgata tcaaattgga cggcacggtg
    27841 caaaaggctc tcgaaaacct tggtttggga gaaggctctg cattacctgt tggggtgcct
    27901 gttccgtggc cctcagccac tccgccaaca ggctggctga aatgcaacgg agcagctttt
    27961 tctgctgaaa aatacccgga actggcaaag gcttacccga caaataaatt gcctgattta
    28021 cgcggtgaat ttattcgtgg ctgggatgac ggacgtggta tggatacggg gagggcaata
    28081 ttatccgctc agggcgatgc catacgtaat atctatggtg agttcaagac tgtaaacacc
    28141 gaaaattatt caatatggga atcagcaggc tcgtttaagg gggcagtagt gcctttgaac
    28201 ccctcaacga acaatagtta tttctcctta atcagaagta tggtgacaga aagaacagat
    28261 ggcgctgttt acccaaaagt gattggcctt gatgcttcaa gaattgttcc aactgcaaac
    28321 gaaaaccgtc ctcgtaacat tgcctttaat tatatcgtga gggctgcctg atgaataaag
    28381 ccgtattaaa taacgaactc attgccataa aagcgggagt cattaccatt tataattatg
    28441 atggtgaaac gcgggagtat atttccacat caactgaata tcttgcggtt ggcgtcggta
    28501 tcccggcatg ttcttgttta gatgcaccag ttacacataa agctggttat gcaatctgcc
    28561 gttctgcaga ttttaactca tgggaatatg tgccagacca tcgcggtaaa atcatctata
    28621 gcaccgaaac aggagaatcg aaagaaatca cagctccggg tgattatcct gaaaatacaa
    28681 ccactatcgc cccgttaacg ccatacgata aatgggatgg tgagaaatgg gtgacggata
    28741 ccgaggcaca gcatagcgcc gcagtagaag cggcagaagc acagcgccag tcgctgattg
    28801 atgctgcaat ggcttccatc agtctgattc agctgaaatt acaggccggg cggaagctga
    28861 cgcagccaga aaacacccga cttaacgctg tgctggatta tattgacgcg gtgacggcaa
    28921 cagataccag cacagcgcc
//
LOCUS       DAZFFG010000056        28738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFFG010000056 DAZFFG010000000
VERSION     DAZFFG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.056"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..615
                     /locus_tag="ACHJ7J_002712"
     CDS             <1..615
                     /locus_tag="ACHJ7J_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000927.1"
                     /translation="YKFLLSGNAGEMILYFANVFFNPLALSFIIATIICITIKKRSSR
                     SFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIVKQGPTLV
                     TRDAHAILAIVAFSQNELGVHSIEELTAIQNGTAEMHVCDVQGFACAYQEGVQTMSDG
                     KVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            738..1364
                     /locus_tag="ACHJ7J_002713"
     CDS             738..1364
                     /locus_tag="ACHJ7J_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000928.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1403..1648)
                     /gene="zapB"
                     /locus_tag="ACHJ7J_002714"
     CDS             complement(1403..1648)
                     /gene="zapB"
                     /locus_tag="ACHJ7J_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO5000929.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2074..2919
                     /gene="glpF"
                     /locus_tag="ACHJ7J_002715"
     CDS             2074..2919
                     /gene="glpF"
                     /locus_tag="ACHJ7J_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO5000930.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2942..4450
                     /gene="glpK"
                     /locus_tag="ACHJ7J_002716"
     CDS             2942..4450
                     /gene="glpK"
                     /locus_tag="ACHJ7J_002716"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO5000931.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDEKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4680..5690
                     /gene="glpX"
                     /locus_tag="ACHJ7J_002717"
     CDS             4680..5690
                     /gene="glpX"
                     /locus_tag="ACHJ7J_002717"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5000932.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVNVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5787..6533
                     /gene="fpr"
                     /locus_tag="ACHJ7J_002718"
     CDS             5787..6533
                     /gene="fpr"
                     /locus_tag="ACHJ7J_002718"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO5000933.1"
                     /translation="MADWVTGKVTKVQNWTEALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPINKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6538..6966)
                     /gene="uspD"
                     /locus_tag="ACHJ7J_002719"
     CDS             complement(6538..6966)
                     /gene="uspD"
                     /locus_tag="ACHJ7J_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO5000934.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(6993..7292)
                     /gene="yiiS"
                     /locus_tag="ACHJ7J_002720"
     CDS             complement(6993..7292)
                     /gene="yiiS"
                     /locus_tag="ACHJ7J_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO5000935.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7504..7944)
                     /gene="yiiR"
                     /locus_tag="ACHJ7J_002721"
     CDS             complement(7504..7944)
                     /gene="yiiR"
                     /locus_tag="ACHJ7J_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5000936.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            8045..8644
                     /gene="yiiQ"
                     /locus_tag="ACHJ7J_002722"
     CDS             8045..8644
                     /gene="yiiQ"
                     /locus_tag="ACHJ7J_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO5000937.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8752..9519
                     /gene="tpiA"
                     /locus_tag="ACHJ7J_002723"
     CDS             8752..9519
                     /gene="tpiA"
                     /locus_tag="ACHJ7J_002723"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO5000938.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9574..10329)
                     /gene="cdh"
                     /locus_tag="ACHJ7J_002724"
     CDS             complement(9574..10329)
                     /gene="cdh"
                     /locus_tag="ACHJ7J_002724"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO5000939.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10436..11425)
                     /gene="sbp"
                     /locus_tag="ACHJ7J_002725"
     CDS             complement(10436..11425)
                     /gene="sbp"
                     /locus_tag="ACHJ7J_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO5000940.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11745..12707)
                     /gene="pfkA"
                     /locus_tag="ACHJ7J_002726"
     CDS             complement(11745..12707)
                     /gene="pfkA"
                     /locus_tag="ACHJ7J_002726"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO5000941.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12888..13790)
                     /gene="fieF"
                     /locus_tag="ACHJ7J_002727"
     CDS             complement(12888..13790)
                     /gene="fieF"
                     /locus_tag="ACHJ7J_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO5000942.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(13939..14439)
                     /gene="cpxP"
                     /locus_tag="ACHJ7J_002728"
     CDS             complement(13939..14439)
                     /gene="cpxP"
                     /locus_tag="ACHJ7J_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO5000943.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            14589..15287
                     /gene="cpxR"
                     /locus_tag="ACHJ7J_002729"
     CDS             14589..15287
                     /gene="cpxR"
                     /locus_tag="ACHJ7J_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO5000944.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            15284..16657
                     /gene="cpxA"
                     /locus_tag="ACHJ7J_002730"
     CDS             15284..16657
                     /gene="cpxA"
                     /locus_tag="ACHJ7J_002730"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO5000945.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(16763..17437)
                     /gene="yiiM"
                     /locus_tag="ACHJ7J_002731"
     CDS             complement(16763..17437)
                     /gene="yiiM"
                     /locus_tag="ACHJ7J_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO5000946.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(17586..18569)
                     /gene="kdgT"
                     /locus_tag="ACHJ7J_002732"
     CDS             complement(17586..18569)
                     /gene="kdgT"
                     /locus_tag="ACHJ7J_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO5000947.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(18829..19449)
                     /gene="sodA"
                     /locus_tag="ACHJ7J_002733"
     CDS             complement(18829..19449)
                     /gene="sodA"
                     /locus_tag="ACHJ7J_002733"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO5000948.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            19734..20768
                     /gene="rhaT"
                     /locus_tag="ACHJ7J_002734"
     CDS             19734..20768
                     /gene="rhaT"
                     /locus_tag="ACHJ7J_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO5000949.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(20765..21613)
                     /gene="rhaR"
                     /locus_tag="ACHJ7J_002735"
     CDS             complement(20765..21613)
                     /gene="rhaR"
                     /locus_tag="ACHJ7J_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO5000950.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(21687..22523)
                     /gene="rhaS"
                     /locus_tag="ACHJ7J_002736"
     CDS             complement(21687..22523)
                     /gene="rhaS"
                     /locus_tag="ACHJ7J_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO5000951.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASSLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            22811..24280
                     /gene="rhaB"
                     /locus_tag="ACHJ7J_002737"
     CDS             22811..24280
                     /gene="rhaB"
                     /locus_tag="ACHJ7J_002737"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO5000952.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMTQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            24277..25536
                     /gene="rhaA"
                     /locus_tag="ACHJ7J_002738"
     CDS             24277..25536
                     /gene="rhaA"
                     /locus_tag="ACHJ7J_002738"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO5000953.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDIEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            25987..26811
                     /gene="rhaD"
                     /locus_tag="ACHJ7J_002739"
     CDS             25987..26811
                     /gene="rhaD"
                     /locus_tag="ACHJ7J_002739"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO5000954.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            26821..27135
                     /gene="rhaM"
                     /locus_tag="ACHJ7J_002740"
     CDS             26821..27135
                     /gene="rhaM"
                     /locus_tag="ACHJ7J_002740"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO5000955.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(27176..28570)
                     /locus_tag="ACHJ7J_002741"
     CDS             complement(27176..28570)
                     /locus_tag="ACHJ7J_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5000956.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMLKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         7155 a         7358 c         7196 g         7029 t
ORIGIN      
        1 tacaaatttc ttttatctgg caacgcgggt gaaatgattt tatattttgc taacgtattt
       61 tttaacccac ttgccttatc atttattatc gccacaatca tctgtataac gataaaaaaa
      121 agaagcagcc gcagctttat tcgcggcact tgttggttaa tgggattatt tcttatctat
      181 agcagctata cggtgtggga acgacacagc atatgggatt atactttccc ggagccaggt
      241 atcaccgtca cggttccgag caaacagtgg gttgccaata tagtaaaaca aggaccaacc
      301 ctggtgaccc gggatgccca tgctatttta gctattgttg ccttctcaca aaatgaactc
      361 ggtgtgcatt caatcgagga acttaccgcg attcaaaacg gaactgcaga aatgcatgtt
      421 tgtgatgttc agggatttgc ctgcgcttat caggaaggag tgcaaacaat gagtgatggc
      481 aaagtaagac atgctatttt catgactcta ctggataaca ctaatttgat acaactggtc
      541 gcagctatcg acccagatta tttggacgaa tatcaagaag aagtaatgaa aatgatgctt
      601 tctgctcgtc aatgatctga tgtgcatgtt aataataatg gattgatatg agatacgtta
      661 tcttattaat actatggata ataactgttt ttcttagtca tcttcagcac agtaacttgc
      721 ttactggaga tcctgggatg gttggcgagt atatcggttc agtacttctt gggccgttat
      781 tacttcccgt gcttgtgtca ggtattctgt gcacattcac taaaaaaacg cgtaacttcg
      841 ccagttttac tcgcgggtgc tgttgggtat tgggagtcct gcttttatcc aacgtcggca
      901 atacgtttcg tctgttcacc ccgtggcact atacgtttga gaaggcagct atttccgtga
      961 ctgtcccaaa ccgacactgg aatacggttt ctattagtac tgataaaacc attgatataa
     1021 gaagtgaaga taatagcgtc tttatttctg ctttccgcct gcccgcagga cgtagcgccg
     1081 atgactcgct ggaagagtta aagaagatgc agcgagacaa tctcaaggat caatacaatg
     1141 aagaaacctt ccagtttcat gattgtaatg ccaaacattt cacctgtaag tatcaggatg
     1201 tgttgataaa ttttgatggt cagcagaaaa gaaccatcag tgtttatctt gaagacaccc
     1261 ccagggctgt ggggatcatt gcattaatgg agccagatac ggcagataaa tatcgccaac
     1321 aggcgatgga gattatgttg tctgctaaaa atacagtgaa gtaaaaaata aggcgccttg
     1381 cggcgcctct gcgtggaaga gattaaacct cttccatgcg acccagcagg gcctgcagac
     1441 gttcctgcca gccgttctgc tgttctttca gatggttgtt ctcacgctcc agctcttcgc
     1501 gctgatgctg ggcattttga acttcctgcg acagtgagtt gtttttttct ttcagctctt
     1561 cgatttccat ctgcaacaga gtgatggtat caatcgcctg ctgtactttt gcttccagtt
     1621 tctcaaacac ttctaatgac attgtcatac ctctcctgaa ttgcaaggcg ttgatggata
     1681 aaaaatcctc gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc
     1741 tccgattgta tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg
     1801 ccacactttt catccttctc ctggtgacat aatccacacc aatcaaaaat gttaataaat
     1861 ttgttgcgcg aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaatggcgc
     1921 gataacgctc attttatgac gaagcacaca cattttaagt tcgatatttc tcgtttttgc
     1981 tcgttaacga taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc
     2041 atacaacaaa cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc
     2101 cagtgcattg ctgaattcct cggtaccggg ttgttgattt ttttcggtgt gggttgcgtt
     2161 gcagcactaa aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga
     2221 ctgggggtgg caatggccat ctacctgacc gcaggggttt ccggcgcgca tcttaacccc
     2281 gctgttacca ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt
     2341 atcgtttcac aagttgccgg cgctttctgt gctgcggctt tagtttacgg gctttactac
     2401 aatttatttt tcgacttcga gcagactcat cacattgttc gcggcagcgt tgaaagtgtt
     2461 gatctggcag gcactttctc tacttaccct aatcctcata tcaattttgt gcaggctttc
     2521 gcagttgaga tggtgattac cgctattctg atggggctga tcctggcgtt aacggacgat
     2581 ggcaacggtg taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc
     2641 attggcgcat ctatgggccc attgacgggt tttgccatga acccagcgcg tgacttcggt
     2701 ccgaaagtct ttgcctggct ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac
     2761 attccttact tcctggtgcc gctttttggc cctatcgttg gcgcgattgt aggtgcattt
     2821 gcctaccgca aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag
     2881 gagaccacaa ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa
     2941 catgactgaa aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
     3001 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
     3061 cccaaaacca ggctgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
     3121 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
     3181 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
     3241 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
     3301 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
     3361 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
     3421 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
     3481 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
     3541 ggacgaaaaa atgctggagg tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
     3601 ttcttccgaa gtgtacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
     3661 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
     3721 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
     3781 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
     3841 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
     3901 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
     3961 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggatccgta
     4021 tgcgcgtggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
     4081 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
     4141 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
     4201 gcagttccag tccgatattc tcggcacgcg cgttgaacgc ccggaagtgc gcgaagtgac
     4261 tgcgttgggt gcggcctatc ttgctggtct ggcggtcggt ttctggcaga acctcgacga
     4321 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
     4381 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
     4441 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
     4501 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
     4561 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcacccccg cacacatcaa
     4621 taatcccacc cttcccctgt gctacacttc gcaccattcc ttactgctta gagtttgcta
     4681 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
     4741 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
     4801 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
     4861 acgaagcacc gatgctctac attggtgaaa aagtcggtac cggtcgcggc gacgcagtag
     4921 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggtcag gctaacgcgc
     4981 tggcggtgct ggcagtaggc gataaaggct gtttcctcaa cgcgccagat atgtatatgg
     5041 agaagctgat cgtcggaccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
     5101 ataacctgcg caatgtcgcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
     5161 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
     5221 tatttgctat tccggatggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
     5281 aagttaacgt gctgtacggt attggtggcg cgccggaagg cgtagtttct gcggcggtga
     5341 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
     5401 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
     5461 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
     5521 ctgccaccgg tattaccaaa ggcgatctgc tggaaggcat tagccgcaaa ggcaatatcg
     5581 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcta
     5641 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca catcctctga ttgatttgat
     5701 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataaga
     5761 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggtaaa gtcactaaag
     5821 tgcagaactg gaccgaagcc ctgtttagtc tcaccgttca cgctcccgtg cttccgttta
     5881 ccgccgggca attcactaaa cttggccttg aaattgacgg cgaacgcgtc cagcgcgcct
     5941 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
     6001 atggcaaatt aagcccacga ttggcggcac tgaaaccagg agatgaagtg caggtggtta
     6061 gcgaagcagc tggcttcttt gttctggatg aagtgccaga ttgcgaaacg ctatggatgc
     6121 tggcaaccgg tacagcgatt ggcccttatt tatcgattct acaactaggc aaagatttag
     6181 atcgcttcaa aaatctggtc ctggtgcacg ccgcacgtta tgccgccgac ttaagctatt
     6241 tgccactgat gcaagaactg gaaaaacgct acgaaggaaa actgcgcatt cagacggtgg
     6301 tcagtcggga aacggcagcg ggttcgctca ccggacggat accggcgtta attgaaagtg
     6361 gtgaactgga aagcgcggtt ggcctgccga taaataaaga aaccagccat gtgatgctgt
     6421 gcggcaatcc acagatggtg cgcgatacgc aacagttgct gaaagagacc cggcagatga
     6481 cgaaacattt acgtcgccga ccgggtcaca tgacagcgga gcattactgg taagcggtta
     6541 cttatcgata aacggcacga tgagcaaatc cgcactcaac ttattgatca tcccgcgata
     6601 tgccggcatc aaacggttga taaatgagtg gtgatgacca cagacaagga ggtcgcactg
     6661 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
     6721 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
     6781 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
     6841 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
     6901 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
     6961 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
     7021 aaaataatca tctcggcctg gcatgagaag gtgaaggccg catcaagctc aacttgcccc
     7081 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
     7141 gcaagctctt tcagtttggt cataaaggac tctgcttctt cgcgggtggc aaaaaagcgc
     7201 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
     7261 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
     7321 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
     7381 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggcat
     7441 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
     7501 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
     7561 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
     7621 caacgtcggg acaaaacgcc ctaccgccca ttgccagaca cccggtaaaa tcgcccagtt
     7681 acccgccagt agcatccacg ctacaatcat cagaaatgcc catgcgccgg aacgcccgcg
     7741 atcatgcaaa cgcttaacgg ttactgccgc tgttggccag agcaagcaca caaggcaaaa
     7801 cgccgcggtc tgaatatcga gtaaattttt acccgccagt gagaaaagca ccagcatgcc
     7861 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
     7921 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
     7981 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
     8041 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
     8101 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
     8161 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
     8221 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
     8281 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
     8341 aaaaatcaaa agcattcaaa tgacatggct acccatccag gggccagagc aaaaagccgc
     8401 gaaagcgaaa gctcaggaat acatggcagc ggtgatccgc acactcaccc cattaatgac
     8461 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
     8521 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
     8581 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
     8641 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
     8701 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
     8761 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
     8821 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
     8881 gaaatgtaca tcgatatggc gaagcgtgaa gctgaaggca gccacatcat gctgggtgcg
     8941 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacctctgc tgctatgctg
     9001 aaagacatcg gcgcacagta catcatcatc ggtcactctg aacgtcgtac ttaccacaaa
     9061 gaatctgacg aactgatcgc gaaaaaattc gcggtgctga aagagcaggg cctgactccg
     9121 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gcaaaactga agaagtttgc
     9181 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
     9241 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
     9301 caggctgttc acaaattcat tcgtgaccac atcgctaaag ttgacgctaa catcgctgaa
     9361 caagtgatca ttcagtacgg cggttctgta aatgcttcta atgctgcaga actgttcgct
     9421 cagccggata tcgacggcgc gctggttggt ggtgcttctc tgaaagctga cgccttcgca
     9481 gtaatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
     9541 atatccggca cttactttcc ttaactcttc gccttaacgc aaaatctcac actgatgatc
     9601 ctgaatttcc tcggctgaag cacggttaag tgtcagtaga tttcgttgtg tcgccagcaa
     9661 cacaaacgaa ttatcactct gccgcaccat cgccagcccg tagcttccca tatgttcccg
     9721 cgcctcaggc acttcttccg ccagcatcat aaatgggctg cgttgtacca gttcgctttc
     9781 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
     9841 gctgctgatg ttcgccagat tgttatccag ctgttcgcgc acatcaggac gaatacaaga
     9901 gatatgtata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
     9961 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    10021 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    10081 acgatacgtt ggcatcaaca gatattgcag tgggccatta agatctttta aaaccacgta
    10141 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    10201 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    10261 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    10321 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    10381 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata atgatcctgg ggaaatcagc
    10441 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    10501 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    10561 ttttcgccac ctcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    10621 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttagtacct tttttctcga
    10681 ccactttatc gaccaccgac acggttggct ctgcgaggat agactcactc ggcgtgacga
    10741 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcaa
    10801 tcagtacatc gccaattccg cgctcgacaa aagtgttagt ggagccgcgc gcgccagaat
    10861 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    10921 tgttgttgtg atgcagcgcg tagccccagg ctgccaggta gttccagcgc gcgccaccag
    10981 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    11041 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    11101 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    11161 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    11221 tcgcttgttt gcctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    11281 gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    11341 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    11401 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    11461 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    11521 gtatatgttg aggggttaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    11581 ctttatagat gccggatgag gcgcgaacgc cttatccggc ctacaaaagt ttgtaaattc
    11641 aataaattgc agaattcacg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    11701 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    11761 gcgcagtcca gccagtcgcc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    11821 tcgtggtgaa ccagctgttc gttctgaata cctacgcaac gaccgccgta acctgccagc
    11881 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    11941 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    12001 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    12061 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    12121 tcaacttccg gaaccacaac gaattcacag ccaccagcaa tagccgcagc caacgtcaga
    12181 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    12241 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    12301 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca ggccgatgca cgggaagccc
    12361 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    12421 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    12481 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agaaacgctg
    12541 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaatgccc
    12601 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    12661 cctggcgcat caccgccgct tgtcaacaca ccgattttct taatcatgac tacctctgaa
    12721 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    12781 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    12841 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    12901 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    12961 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    13021 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    13081 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    13141 tcttgccgtt cctcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    13201 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    13261 cgatgccagc cgtaccagga caaccccagc gcgagcagaa ttgcgccgtt catcataaca
    13321 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    13381 caacgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    13441 ccgacgcctg gatctgtcat cggtgttgga gatatcagat gttgaatgcc tgtcaaaaac
    13501 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    13561 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg cactaccaat
    13621 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    13681 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcagcga agccatcgcc
    13741 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    13801 gctatcaact gacgctagta taacggaagc aaatcatccg caatgcatta agcagcaggc
    13861 aaattgagga taaaaaaaac ccccacatca tgtgggggaa gacagggatg gtgtctatgg
    13921 caaggaaaac agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga
    13981 tgaacttttt tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc
    14041 gtttaaaacc gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc
    14101 catctcaacc tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc
    14161 gttttcatca aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt
    14221 aacaggaggc tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg
    14281 ttcggttaaa cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc
    14341 acccggatgc cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc
    14401 cagcgttgag gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc
    14461 tgtcgcgatt caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt
    14521 aaaacaacgt aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta
    14581 tttaaacaat gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa
    14641 aggagctgct cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc
    14701 ttgatcttct ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa
    14761 atggtatcga cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga
    14821 cggcgcgcgg cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc
    14881 tcccgaaacc gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt
    14941 cgcactggag cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg
    15001 ccttagtgct gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa
    15061 ccggtactga gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc
    15121 gtgaacattt aagccaggaa gtgctgggca aacgcctgac gcctttcgac cgcgctatcg
    15181 atatgcacat ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt
    15241 ttaaaacctt gcgtggtcgc ggctacctga tggtttctgc ttcatgatag gcagtttaac
    15301 cgcgcgcatc ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat
    15361 gttacccaag ctcgattcac gccagatgac cgagcttctg gatagcgaac agcgtcaggg
    15421 gctgatgatt gagcagcatg ttgaagcgga gctggcgaac gatccgccca acgatttaat
    15481 gtggtggcgg cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt
    15541 attggtgacc accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg
    15601 taactttatt ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt
    15661 ggaactggtc ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat
    15721 tcgtccggcc agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt
    15781 actgctgatt gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct
    15841 ggcaaaaccg gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg
    15901 ccagcacccg gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa
    15961 ccagatggtc accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat
    16021 ctctcacgag ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg
    16081 tcgtagtggt gaaagcaagg aactggagcg tattgaaacc gaagcgcaac gtctggacag
    16141 catgattaac gacctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga
    16201 gaccatcaaa gccaatcagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga
    16261 gcaaatgggc aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa
    16321 cccgaacgcc ctggagagtg cgctggaaaa cattgttcgt aatgccctgc gttattccca
    16381 tacgaagatt gaagttggct ttgcggtaga taaagacggt atcaccatta cggtggacga
    16441 cgatggtcct ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac
    16501 cgatgaagca cgcgatcgtg aatctggcgg tacaggtttg gggctggcga ttgttgaaac
    16561 cgccattcag cagcatcgtg gctgggtgaa agcagaagac agcccgctgg gcggtttacg
    16621 gctggtgatt tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata
    16681 aggcgtttac gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct
    16741 tatcaggcct acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct
    16801 tgccgctcag tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg
    16861 agagtaaacg gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct
    16921 cctgcacggt cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt
    16981 tccctggcgc tatcacgcta tacaaccatc ccactttacc cgtgttttgc atcaactgcg
    17041 ctatatcgct gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga
    17101 cctgaattaa tgcctctccc cagcggaaaa tatcgccaat ataaacatta ctttccgtga
    17161 gaccgtcggt tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg
    17221 gaaattcccg cgcccagtag agataatgct cacgaggata atgacacagc gctctgtctg
    17281 gtccgccgtg aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca
    17341 tcaactcacc atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct
    17401 gaatcttgcc tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt
    17461 tccccattag accatattta ttttatgtca ggcgtgctgt tttcgcggta agtatccagg
    17521 caggaactca atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag
    17581 caggtttatt tcaccgtacc taaaatttcg attttcgccg ctctggcttt gactttacgt
    17641 gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgactgc cgtcgctacc
    17701 agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca
    17761 ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca
    17821 ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat tactgccaca
    17881 cccaacagga tccccagcaa acctgtctgg gcaattacag tcaaatcaat ggtattgcct
    17941 agtgcgaagg caaagaatgg aatcagcgtc tgcaccgctt tgctgaaaaa ttctcgtaat
    18001 tcagggtcaa ggttcccaag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa
    18061 acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc
    18121 ccggactcca acgacatcaa cacaaatgcc ccagcttctt cttttgtgcc gtactgctgc
    18181 atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag tgccagcgtt
    18241 gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatacg cgaggcaatt
    18301 gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt
    18361 accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga
    18421 atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc
    18481 ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg
    18541 attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc
    18601 tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt
    18661 aatcaataaa agtgatcaaa accacatcaa ttgaaacgct gttttatttt tatcggatca
    18721 ttgtttttat tttgaaattg atcacaaaaa aaccaccgcc attggcgatg gttcattttt
    18781 taagctgata tgcggcgtaa acgcctcatt gcagcagacg gcaaatgatt attttttcgc
    18841 cgcgaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc
    18901 cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat
    18961 cgggaagccg gaagcgccag aaatagcttc acccatcagc ggggagtcct ggttagcagt
    19021 agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg
    19081 ggaagctgcc gctttctcaa attctgcttt gaagttatct acggaaccga aatcacgttc
    19141 gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag
    19201 gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag
    19261 ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc
    19321 cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat
    19381 ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt
    19441 atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt
    19501 tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt acttttcgta
    19561 agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccaa ttaatgccgt
    19621 gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt
    19681 tacactcatc tcgtcggaga tgtgacgcga cgaaaaatga tgaggataag aagatgagta
    19741 acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt
    19801 acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga
    19861 ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg aatttctggg
    19921 cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt
    19981 gggggatcgg taatatcaac tacggcctga ccatgcgtta tctcggcatg tcgatgggaa
    20041 ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg
    20101 gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttgctc ggcgttctgg
    20161 tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg
    20221 gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctagcggtg atgtgcggca
    20281 ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg
    20341 ccgcacttgg cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg
    20401 gcggcgcgat catcaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt
    20461 cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg
    20521 cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctggggc cacgcccgca
    20581 ttccggcgca atatgactac atcagttgga tgctgcatat gagcttctat gtattgtgcg
    20641 gcggtatcgt cgggctggtg ctgaaagagt ggaacaatgc aggacgccgt ccggtaacgg
    20701 tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg
    20761 cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc ccggtttccc
    20821 gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc gaaatatcac
    20881 tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg acctgccgca
    20941 gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac
    21001 tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg ctggccgcca
    21061 gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac gaatcactgg
    21121 tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactct gccatttcgt
    21181 tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc tgccgcgcct
    21241 gtgccatccc catgctacct aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg
    21301 gaatcgcccc ctgccagtca agattcagct tcagccgctc cgggcaataa ataatattct
    21361 gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga atgtaaaaga
    21421 gatcgccacg ggtaatgcga taagggcgat cgttgagtac atgcagacca ttaccgcgcc
    21481 agacaatcac cagctcacaa aaatcatgtg tatgttcagc aaagacatct tgcggataac
    21541 ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg agaagtttta
    21601 actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc gcaatatggc
    21661 gtacaaatac gttgagaaga ttcgcgttat tgcagaaaac catcccgtcc ctgacgaata
    21721 tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg
    21781 aatccgcagc gataggcgat gtcagtaacg ctggcctcgc tgtggcgtag cagatgtcgg
    21841 gctttcatca gtcgcaggcg gttcaggtaa cgctgaggcg tcagtcccgt ttgctgctta
    21901 agctgccgat gtagcgtacg cagcgaaaga gaaaattgat ccgccacggc atcccaattc
    21961 acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagact tgatgcgctg
    22021 ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcaataattg cataaacaag
    22081 atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc catctgtgca
    22141 accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga cggatactgc
    22201 ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa tcgatccggc
    22261 gatcgataca gcacattggt cagacacaga ttatcggtat gttcatacag atgccgatca
    22321 tggtcgcgca caaaacagac cgtgccaccg gtgatggtat agggctgccc attaaacaca
    22381 tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg atgttcagga
    22441 aaatccgcct gcgggagccg gggttctatc gccacggacg cgttaccaga cggaaaaaaa
    22501 tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg cgaaatagta
    22561 atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat
    22621 ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca ccacaattca
    22681 gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt
    22741 cagtaacgag aaggtcgcga attcaggcgc tttttagact agtcgtaatg aaattcagca
    22801 ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg
    22861 cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg
    22921 ttttaacaat gggctgcata gtcagaacgg ctatgtcacc tgggatgtgg atagccttga
    22981 aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta tcgatagcat
    23041 tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc agcgtgtggg
    23101 cctgcccgtt gcttatcgcg acagccgcac caatggcctg atgacgcagg cacaacagca
    23161 actcggcaaa cgcgatattt atcaacgtag cggcatccag tttctgccct tcaatacgct
    23221 ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca ttgctcacgc
    23281 tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact gggagtacac
    23341 caatgccaca accacgcaac tggtcaatat caatagcgac gactgggacg agtcgctact
    23401 ggcgtggagc ggtgccaaca aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat
    23461 aggtcactgg atttgcccgc agggtaatga gattccagtg gtcgccgttg ccagccatga
    23521 taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt atctctcttc
    23581 tggcacctgg tcattgatgg gttttgaaag ccagacgcca tttaccaatg acacggcgct
    23641 ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat
    23701 tatgggctta tggctgcttc agcgagtgct tcaggagcgg caaatcaacg atcttccggc
    23761 gcttatcgcc gcgacacagg cacttccggc ttgccgcttc attatcaatc ccaatgacga
    23821 tcgctttatt aatcctgaga cgatgtgcag cgaaattcag gctgcgtgtc gggaaacggc
    23881 gcaaccgatc ccggaaagtg atgctgaact ggcgcgctgc attttcgaca gtctggcgct
    23941 gctgtatgcc gatgtgttgc atgagctggc gcagctacgc ggtgaagatt tctcgcaact
    24001 gcatattgtc ggcggaggct gccagaacac gctgctcaac cagctatgtg ccgatgcctg
    24061 cggtattcgg gtgatcgccg ggcctgttga agcctcgacg ctcggcaata tcggcatcca
    24121 gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg tcagcaccac
    24181 cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact atgtggcgca
    24241 gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact ggaacaggcc
    24301 tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atatcgagga ggcgctgcgc
    24361 caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt ttccggtttt
    24421 gaaaacccgg aaggttcgct gaccggaggg attcaggcta ctggcaatta tccgggcaaa
    24481 gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct gattccgggg
    24541 ccaaaacggc ttaatttaca tgccatctat ctggaatcag acacgccagt ctcgcgcgat
    24601 cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa tcagctcggt
    24661 ctggatttta acccctcctg tttttcgcat ccgctaagcg ccgatggctt tacgctttcc
    24721 catgccgacg acagcattcg ccagttctgg attgatcact gcaaagccag ccgtcgcgtt
    24781 tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg gatcccggat
    24841 ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct ggcagcactg
    24901 gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt tgagagcaaa
    24961 ttgtttggca ttggcgcgga gagctacacg gttggctcca atgagtttta cctgggttat
    25021 gccaccagcc gccagacggc gctgtgcctg gacgccgggc atttccaccc gactgaagtg
    25081 atttccgaca agatttccgc cgccatgctg tatgtgccgc agttgctgct gcacgtcagc
    25141 cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac ccaggcgatt
    25201 gccagtgaga ttgttcgtca cgatctgttc gaccgggtgc atatcggcct tgacttcttc
    25261 gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat gaaaaaagcc
    25321 ctgctacgtg cgttactgga acctaccgct gaactacgca agctggaagc ggcgggcgat
    25381 tacactgcgc gtctggcgct actggaagag cagaaatcgt tgccgtggca ggcagtatgg
    25441 gaaatgtatt gccaacgtca tgatacgcca gcaggtagcg aatggctgga gagcgtgcgg
    25501 gcatatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatac ggcacgagtg
    25561 ccttatccgg cctacaggtc ggcaatagtt gtaggcctga taagacgcga cagcgtcgca
    25621 tcaggcattg attgccggat gcggcacgag cgccttatcc ggcctacagg tcggcaatag
    25681 ttgtaggcct gataagacgc gacagcgtcg catcaagcat tgattgccgg atgcggcacg
    25741 agcgccttat ccggcctaca ggtcggcaat agttgtaggc ctgataagac gcgacagcgt
    25801 cgcatcaggc attgattgcc ggatgcggca caagtgcctt atccggccta caggtcggca
    25861 atagttgtag gcctgataag acgcgacagc gtcgcatcag gcattgattg ccggatgcgg
    25921 cacgagcgcc ttatccggcc tacgggtcgt gcatccgaca acaccgaatt tacaggaaca
    25981 cagaacatgc aaaatattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc
    26041 gacgcctggc tgaaaggctg ggatgagcgc aacggcggca acctgacgct gcgcctggat
    26101 gacgccgata tcgcaccata tcacgacaat ttccaccaac aaccgcgcta tatcccgctc
    26161 agccagccca tgcctttact ggcaaataca ccgtttattg tcaccggctc cggcaaattc
    26221 ttccgtaacg tccagcttga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgac
    26281 ggcgcgggct accatattct ctgggggtta accaacgaag ccgttccaac ttctgaactt
    26341 ccggctcact tcctttccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg
    26401 atcatgcact gccacgccac caacctgatc gccctcacct atgtactgga aaacgacacc
    26461 gcggtcttta ctcgccaact gtgggaaggc agcaccgagt gtctggtggt attcccggat
    26521 ggcgttggca ttctgccgtg gatggtaccc ggcacggacg aaatcggcca ggcaactgcc
    26581 caagagatgc aaaaacattc gctggtgttg tggcccttcc acggtgtgtt cggttccgga
    26641 ccgacgctgg atgaaacctt cggtttaatc gacaccgcag agaaatcagc ggaagtatta
    26701 gtgaaggttt attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg
    26761 ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc tggcgctgta aggagcaaac
    26821 atgatccgca aagcctttgt catgcaggtt aaccccgacg cccacgaaga gtatcagcgt
    26881 cggcataacc ccatctggcc ggaactggaa gcagtgctga aatctcacgg tgcgcataac
    26941 tacgccattt acctcgacaa agcgcgtaat ctgctgtttg ccatggtaga gattgaatct
    27001 gaagaacggt ggaatgcggt agccagcact gaaatctgcc aacgttggtg gaaatatatg
    27061 accgacgtta tgcccgctaa cccggataac agcccggtga gtagcgagct gcaagaagtg
    27121 ttttacctgc cttaatgagt tgcggtgccg gaaaatatcc agcaccgcga cttcatcaga
    27181 agaagtattt aaagcgaacg cgtgcgccgt aacggtcatc agtatcatca ccgtgttttt
    27241 tatcgccgtc gctatccaga atggagtaat acgtcccgag gtaaatatta aagttctcca
    27301 tatccatcac attagcgaac tgataagacg catgaatggt gtgaatggtg tacgtccctt
    27361 catcatcaat ccagcagtcg ttatcacaaa ctacaccgct gaattcatca atcttattgt
    27421 gcgcgtagat ataaccgagc tcgaaacgtt tccacagcgc gttaatcccg gcagtgaaat
    27481 ctttctcatt attagcatct aaataggcgg tattaagatt aaccacaatg ccattgtccg
    27541 gatccgtttt caggccgttc caggtcatgc tcataccata gccggtacga tcggactgat
    27601 cgacaaaatt acccttgcta tcggtataac cataagcatt atttaccacg ttcgcttcca
    27661 tcgctgcgga aacggtgaat tcttccgtcg gcgaccaggc aattactggg cgcagataag
    27721 caacattttt ctgctgctcc atatcgcgtc catgataatt accgtcatta tataaagatg
    27781 ttccgtcttc aagtaacgtg tttaattcaa aataccagtt atcgagttgt ttgctgacga
    27841 ggaaattacc gccagcgtta gagcgcccgc ggccctcttt caacatatag atataaccgc
    27901 tgccatcgtc ataaagatca ttcgcagtat taccggaatg ttcaacaaag gtatcctgat
    27961 tcaacgggaa catatcgtag gcttcaaaac ggcctacttt gaccttccag tcgttttctt
    28021 tgccaaagaa gaataccgca tcatcgatat ttactgaacc gtgcatatca cccagcggtt
    28081 gtgcggagaa cccggcgaaa taaccgttgt ccatctttcg cataccgtca aaacctaaca
    28141 gaatacgacc attgagattc cactgttcat tagtgggatc gctattcaca tcagcgttgg
    28201 tcattgccag cagaccatga ttactttccg catccatatt aaattctacg tcgccgtaga
    28261 tttttaaatc gccataacct gaaagcaaaa gattaggtga ggtggcattt ttctttgcag
    28321 attccgccgt tgcctgtcca tttaccggcg tattgccctg agcggcctta atttcgcgga
    28381 tctcagcggc ttgttgtgtc tgcagctgtt taacctggct ctctgctttt gccgcccgca
    28441 tttctgccgc ttccaggcgt tcttccagca atgccatgcg ttgttccagc gttaaatgct
    28501 gtgtttttgc cataccggta ctacatgcca ataataaacc gatggctaaa gataatgtcg
    28561 attttttcat tatttacgtt ccctgaaata aaggaggccc tacgctttcc aaaaggaata
    28621 cgtatataga gaaatataaa gcgtgcactt tcgaattttt aatccaactt cagcaggccg
    28681 atggatagta tcccgccgca taatatgagt aaatccgctg atttcctgga acgtgagg
//
LOCUS       DAZFFG010000057        28378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFFG010000057 DAZFFG010000000
VERSION     DAZFFG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.057"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            163..681
                     /gene="ydeJ"
                     /locus_tag="ACHJ7J_002742"
     CDS             163..681
                     /gene="ydeJ"
                     /locus_tag="ACHJ7J_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO5000957.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVIHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(726..2771)
                     /gene="dcp"
                     /locus_tag="ACHJ7J_002743"
     CDS             complement(726..2771)
                     /gene="dcp"
                     /locus_tag="ACHJ7J_002743"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO5000958.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGSLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2908..3654
                     /gene="ydfG"
                     /locus_tag="ACHJ7J_002744"
     CDS             2908..3654
                     /gene="ydfG"
                     /locus_tag="ACHJ7J_002744"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO5000959.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3743..4429
                     /gene="rspR"
                     /locus_tag="ACHJ7J_002745"
                     /gene_synonym="ydfH"
     CDS             3743..4429
                     /gene="rspR"
                     /locus_tag="ACHJ7J_002745"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO5000960.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4606..4809
                     /gene="ydfZ"
                     /locus_tag="ACHJ7J_002746"
     CDS             4606..4809
                     /gene="ydfZ"
                     /locus_tag="ACHJ7J_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO5000961.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4844..6304)
                     /gene="ydfI"
                     /locus_tag="ACHJ7J_002747"
     CDS             complement(4844..6304)
                     /gene="ydfI"
                     /locus_tag="ACHJ7J_002747"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5000962.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLFLLAHGAKATVAKYSVK"
     gene            complement(6393..7760)
                     /gene="ydfJ"
                     /locus_tag="ACHJ7J_002748"
     CDS             complement(6393..7760)
                     /gene="ydfJ"
                     /locus_tag="ACHJ7J_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO5000963.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            complement(7818..8837)
                     /gene="rspB"
                     /locus_tag="ACHJ7J_002749"
     CDS             complement(7818..8837)
                     /gene="rspB"
                     /locus_tag="ACHJ7J_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO5000964.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIF
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHVDGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWVINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(8849..10063)
                     /gene="rspA"
                     /locus_tag="ACHJ7J_002750"
     CDS             complement(8849..10063)
                     /gene="rspA"
                     /locus_tag="ACHJ7J_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO5000965.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSEGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTITPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VSRLEDGTLWNW"
     gene            complement(10269..10595)
                     /gene="ynfA"
                     /locus_tag="ACHJ7J_002751"
     CDS             complement(10269..10595)
                     /gene="ynfA"
                     /locus_tag="ACHJ7J_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO5000966.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            10730..11071
                     /gene="ynfB"
                     /locus_tag="ACHJ7J_002752"
     CDS             10730..11071
                     /gene="ynfB"
                     /locus_tag="ACHJ7J_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO5000967.1"
                     /translation="MKMTLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            11106..11666
                     /gene="speG"
                     /locus_tag="ACHJ7J_002753"
     CDS             11106..11666
                     /gene="speG"
                     /locus_tag="ACHJ7J_002753"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO5000968.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(11669..12379)
                     /gene="ynfC"
                     /locus_tag="ACHJ7J_002754"
     CDS             complement(11669..12379)
                     /gene="ynfC"
                     /locus_tag="ACHJ7J_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO5000969.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            12487..12792
                     /gene="ynfD"
                     /locus_tag="ACHJ7J_002755"
     CDS             12487..12792
                     /gene="ynfD"
                     /locus_tag="ACHJ7J_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO5000970.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            12990..15416
                     /gene="ynfE"
                     /locus_tag="ACHJ7J_002756"
     CDS             12990..15416
                     /gene="ynfE"
                     /locus_tag="ACHJ7J_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO5000971.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEITLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLEVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            15477..17900
                     /gene="ynfF"
                     /locus_tag="ACHJ7J_002757"
     CDS             15477..17900
                     /gene="ynfF"
                     /locus_tag="ACHJ7J_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO5000972.1"
                     /translation="MKIDTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            17911..18528
                     /gene="ynfG"
                     /locus_tag="ACHJ7J_002758"
     CDS             17911..18528
                     /gene="ynfG"
                     /locus_tag="ACHJ7J_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO5000973.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            18530..19384
                     /gene="ynfH"
                     /locus_tag="ACHJ7J_002759"
     CDS             18530..19384
                     /gene="ynfH"
                     /locus_tag="ACHJ7J_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO5000974.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            19427..20041
                     /gene="dmsD"
                     /locus_tag="ACHJ7J_002760"
     CDS             19427..20041
                     /gene="dmsD"
                     /locus_tag="ACHJ7J_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO5000975.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            20200..21492
                     /gene="clcB"
                     /locus_tag="ACHJ7J_002761"
     CDS             20200..21492
                     /gene="clcB"
                     /locus_tag="ACHJ7J_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO5000976.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TEGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQFSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(21445..22140)
                     /gene="bioD"
                     /locus_tag="ACHJ7J_002762"
     CDS             complement(21445..22140)
                     /gene="bioD"
                     /locus_tag="ACHJ7J_002762"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5000977.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(22265..23485)
                     /gene="mlc"
                     /locus_tag="ACHJ7J_002763"
                     /gene_synonym="dgsA"
     CDS             complement(22265..23485)
                     /gene="mlc"
                     /locus_tag="ACHJ7J_002763"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO5000978.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(23620..24513)
                     /gene="ynfL"
                     /locus_tag="ACHJ7J_002764"
     CDS             complement(23620..24513)
                     /gene="ynfL"
                     /locus_tag="ACHJ7J_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5000979.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTMAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            24620..25873
                     /gene="ynfM"
                     /locus_tag="ACHJ7J_002765"
     CDS             24620..25873
                     /gene="ynfM"
                     /locus_tag="ACHJ7J_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5000980.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(25922..26041)
                     /locus_tag="ACHJ7J_002766"
     CDS             complement(25922..26041)
                     /locus_tag="ACHJ7J_002766"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO5000981.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            26297..26605
                     /gene="asr"
                     /locus_tag="ACHJ7J_002767"
     CDS             26297..26605
                     /gene="asr"
                     /locus_tag="ACHJ7J_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO5000982.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            26698..26781
                     /locus_tag="ACHJ7J_002768"
     CDS             26698..26781
                     /locus_tag="ACHJ7J_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5000983.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            26881..27702
                     /gene="ydgD"
                     /locus_tag="ACHJ7J_002769"
     CDS             26881..27702
                     /gene="ydgD"
                     /locus_tag="ACHJ7J_002769"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO5000984.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(27741..28070)
                     /gene="mdtI"
                     /locus_tag="ACHJ7J_002770"
     CDS             complement(27741..28070)
                     /gene="mdtI"
                     /locus_tag="ACHJ7J_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO5000985.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIATTLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(28057..28377)
                     /gene="mdtJ"
                     /locus_tag="ACHJ7J_002771"
     CDS             complement(28057..28377)
                     /gene="mdtJ"
                     /locus_tag="ACHJ7J_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014883621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO5000986.1"
                     /translation="MTGTLSMKWASVSEGNGGFILMLVMISLSYIFLSFAVKKIALGV
                     AYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLIKSGTRKARKPEQEVN
                     HGAV"
BASE COUNT         7245 a         7235 c         6828 g         7070 t
ORIGIN      
        1 tgtcaaatat tcagagagtt taactcacta aagttaagaa gattgaaaag tcttaaacat
       61 attttcagaa taatcggatt tatatgtttg gaaattatta tattggacga gcatacagaa
      121 aaagcaaatc acctttacat ataatagcgt ggacaaaaaa cagtgaacat taataaagat
      181 aaaattgtac aactcgcaga taccgacact attgaaaacc tgacatccgc gttgagtcaa
      241 agacttatcg cggatcaatt acgcttaact accgccgaat catgcaccgg cggtaagttg
      301 gctagcgccc tgtgtgcagc tgaagataca cccaaatttt acggtgcagg ctttgttact
      361 ttcaccgatc aggcaaagat gaaaatcctc agcgtaagcc agcaatctct tgaacgatat
      421 tctgcggtga gtgagaaagt ggcagcagaa atggcaaccg gtgccataga gcgtgcggat
      481 gctgatgtca gtattgccat taccggctac ggcggaccgg agggcggtga agatggtacg
      541 ccagcgggta ccgtctggtt cgcgtggcat attaaaggcc agaactacac tgcggttata
      601 cattttgctg gcgactgcga aacggtatta gctttagcgg tgaggtttgc cctcgcccag
      661 ctgctgcagt tactgctata accaggctgg cctggcgata tctccaggac agccattggt
      721 ggtgtttata tgttcaagcc acgatgttgc agcatcggca taatctgagg tgccttaccg
      781 cgccattgtc gatacaggcg ttccagatct tcgctgttac ctctggaaag gatcgcctcg
      841 cgaaaacgca gcccattttc acgcgttaat ccgccctgct caacaaacca ctgataacca
      901 tcatcggcca acatttgcgt ccacagataa gcgtaataac ctgcggcata tccgccacca
      961 aaaatatggg cgaaataact gctgcgatag cgtggcggta tagcaggaag atccatattt
     1021 tccgccacca gcgcccgcaa ttcgaaatca tcgacatcct gcattgcttc gttttcttcc
     1081 aggcaatgcc agcgcatatc gagaagtgcg gcgctaagca gttcgctcat ctcataccct
     1141 ttgttgaaca ggctggcatt acgcattttc tgttgcagtt cgtcaggcat tgctgccccg
     1201 ctctgataat gccgggcgta gcgagcgaat acctgcggat gcgttgccca gtgttcgttg
     1261 atttgcgacg gaaattcgac aaaatcacgc ggcgtgttgg tgccggagag cgtggcataa
     1321 cgctggcggg caaaaaggcc gtgcagcgta tgaccaaatt catggaataa ggttatgaca
     1381 tcatcccaga gtaacaacgc aggctcaccg gcagcgggtt tctgataatt gcagacgtta
     1441 taaattaccg gatgcgtttc attaagcgtt gattgctcaa caaaattgcc catccatgca
     1501 ccgccgcttt ttgaatcacg ggcgaagaaa tcaccgtaaa ataacgccag ccccacgcca
     1561 ttatgatcaa aaatttccca cacccgaacg tcaggatggt agacaggaat atcaaaacgt
     1621 tcgacaaact taataccgaa gagctgattc gcggtccaga atacaccttc attcaacacc
     1681 gtgtttaatt caaaatatgg cttgagctgc gcctcatcaa gatcaaattt ctcccgccgg
     1741 acctgttcgg cataaaatgc ccagtcccac ggctgcgcgc taaacccgcc ttgctgctta
     1801 tcgataaccg cctgtatgga ggctaattca tcgctcgcac gttgacgcgc cgctggaaca
     1861 atttcccgca taaagttaag tgctgcttca ggtgtttttg ccatctgatc ggcgattttc
     1921 catgcggcat aatgaggaaa accaagtagt gttgcctgtt gtgcacggat ctccaccaga
     1981 cgttgaatga tagcgcgggt atcattggca tcattttttt ccgctcgcgt ccagcccgca
     2041 ataaacagtt tttcacgcgt cgcacgatcg cgcatttcgg caagcgccgg ttgttgggtg
     2101 gtattcagca gcggaatcag ccatttgtta tccagacctt tctcgcgagc cgcctctgcc
     2161 gccagcgcaa tctcttgctc actcattcct gccagctgcg cgatatcgtt cacaaccaga
     2221 ctgccggatt tatttgctgc cagtaaccgc tggttaaact gactggtcag tgtcgcagct
     2281 tctgtattca gtacttttaa ttttgcttta tcagcttgtg caagtttggc tccggctagg
     2341 acaaaacgtt gatgaatcac ctccaccagg cggatggatt cactatcaag ccccagagat
     2401 tcacggcgtt gccagacagc atctacccgc gcgaataatt caccgttcag atagatatca
     2461 ttagccagtt ccgccagttc agcggaaaac tgctcgtcaa gacgctgtaa ttcatcattg
     2521 gtatgcgccg cagtcatcgc aaaaaagacg ctggtaacgc gggtaagtaa ttctccgctt
     2581 tgttccagtg ccagaatagt attgttgaaa tcaggtgttt gcgggttaag cgcgatggca
     2641 gcaatttctg cccgcttttg cagcattccc tcatcgaatg ccgggcgata gtgatgattg
     2701 gcaatttgat caaaatgggg agccagatac ggcagtgtgc tttgcacaag gaaaggattc
     2761 attgttgtca ttttcttctc ctgaacgcga ggtgttccat agcgtaggct tactgataac
     2821 ggagtgcaat cttgcaaacc agtattaccc gctcttaagc atcccgtgct atgttattga
     2881 cacacaaaag cgttgaggaa cagtgagatg atcgttttag taactggagc aacggcaggt
     2941 tttggtgaat gcattactcg tcgttttatt caacaagggc ataaagttat cgccactggc
     3001 cgtcgccagg aacggttgca ggagttaaaa gacgaactgg gagataatct gtatatcgcc
     3061 caactggacg ttcgcaaccg tgccgctatt gaagagatgc tggcatcgct ccctgccgag
     3121 tggagcaata ttgatatcct ggtaaataat gccggcctgg cgttgggcat ggagcctgcg
     3181 cataaagcca gcgttgaaga ctgggaaacg atgattgata ccaacaataa aggcctggta
     3241 tatatgacgc gcgccgtctt accgggtatg gttgaacgta atcatggtca tattattaac
     3301 attggctcaa cggcaggtag ctggccgtat gccggtggta acgtttacgg tgcgacgaaa
     3361 gcgtttgttc gtcagtttag cctgaatctg cgtacggatc tgcatggtac ggcggtgcgc
     3421 gtcaccgaca tcgaaccggg tctggtgggt ggcaccgagt tttccaatgt ccgctttaaa
     3481 ggcgatgacg gtaaagcgga aaaaacctat caaaataccg ttgcattgac gccagaggat
     3541 gtcagcgaag ccgtctggtg ggtgtcaacg ctgcctgctc acgtcaatat caataccctg
     3601 gaaatgatgc cggttaccca aagctatgcc ggactgaatg tccaccgtca gtaattttta
     3661 tacccggcgt aactgccggg ttattgcttg tcacaaaaaa gtggtagact catgcagtta
     3721 actcactcac aagcaagaac gaatgaccgt cgaaacgcaa cttaatccca cacagcctgt
     3781 caatcagcag atttatcgta tccttcgtcg cgacattgtc cattgcctga ttgccccagg
     3841 cacaccgttg tcggaaaaag aagtttctgt tcgtttcaat gtgtcacgcc agccggttcg
     3901 tgaagccttt attaaactgg cggaaaacgg cctgattcaa attcgtccgc agcgtggcag
     3961 ctacgtcaac aaaatttcca tggcccaggt gcgcaacggc agttttatcc gtcaggccat
     4021 tgagtgcgcg gtggcgcgtc gggcggcgag catgattacc gaaagccagt gctaccaact
     4081 ggaacaaaat cttcaccagc aacgcattgc cattgagcgc aagcaactgg atgatttttt
     4141 tgaacttgat gacaacttcc atcaactcct gacgcagatt gccgactgtc aactggcgtg
     4201 ggataccatt gagaacctga aagcgaccgt tgatcgcgtg cgctatatga gtttcgacca
     4261 cgtttctcca ccagaaatgc tgttacgcca gcatcttgat attttctctg ccctgcaaaa
     4321 acgtgatggc gatgcggtag aacgtgcaat gacgcaacat ttgcaggaaa tcagcgaatc
     4381 cgtgcgccag atccgtcagg aaaacagcga ctggtttagc gaagagtaat tcatttcctc
     4441 tcatcccatc cggggtgaga gtcttttccc ccgccttatg gctcatgcat gcatcaaaaa
     4501 agatgtgagc ttgatcaaaa acaaaaaata tttcactcga caggagtatt tatattgcgc
     4561 ccgttacgtg ggcttcgact gtaaatcaga aaggagaaaa cacctatgac gacctacgat
     4621 cgtaaccgta acgcaatcac cactggcagc cgtgttatgg ttagcggcac cggtcacact
     4681 ggcaagatcc tgtcgattga tactgaaggt ctgaccgctg agcaaatccg ccgcggaaaa
     4741 accgtagttg ttgaaggttg tgaagagaaa ctggcacctc tggacctgat tcgtctcggc
     4801 atgaactaag cgtgtgaatg ccgccgatgg cggcattgct tttttacttc acggaatatt
     4861 tcgccacggt cgctttcgca ccatgcgcta ataaaaacaa gtacgcttcc gtcacttttg
     4921 cagtaaacaa gctattgtct ggcaaatcat caccaaagat cgccttaatc gccagcaatg
     4981 actggacgcg cgctttccct tcggcactac tttgtacagc cttctgaata acaggtaaca
     5041 gtgggtcact gatttcaatc ggatttccct gttcatcaac accaccgaca taacgcatcc
     5101 aacccgcgac gcccagcgcc agcagatcga acttgctgtc atgcgctaga tgccagcgaa
     5161 cagaatccaa catccgctgt ggcaatttct ggctgccatc catcgcaatc tgccaggttc
     5221 gatggcgtaa cgccgggttg ctatagcgtg caattaatcg gttagcgtaa tcttgcaaat
     5281 caacgccctg cactttcagc gtcggcgctt gttcctgcaa catcaagcca tacgccgcat
     5341 gacgataatg ttcatcttcc atacagtcat taatgtgctg atatccggca agataaccca
     5401 gatacgccag gaatgaatga ctgccgttga gcatgcgcaa cttcatctct tcataaggca
     5461 gtacatcgct aaccagttct gctcccgctt tttcccattc cggacgtccg gcaacaaaat
     5521 tatcttctat tacccactgg cggaaaggtt cacaggcaac gccagcagga tcgcgcacac
     5581 cggtaagttg ttcgattttc gccagcgtat cctctgtcac tgcgggcaca atacggtcca
     5641 ccattgttga tgggaaagtc acgttatctt cgatccattg tgccagtttt acatcaacgg
     5701 cttgcgcgta ggaagtgaca acgtcacgca taacatgacc gttttctggc atgttgtcac
     5761 atgacatgac ggtaaatgcg ggaagtcctg ccgctttacg gcgagccagc gcctcaacaa
     5821 tcacccctgt tgctgttttc ggctggtggg gattttgcac gtcggcaacg accatcgggt
     5881 gatcgagcat taactgtccg gtcgccggag agtggaaata ccctttttcg gtgattgtca
     5941 gagagacaat cgcgatttgt ggttcacaca tcgcagccaa cacggtttct aagccatcta
     6001 tctgtacgtg caaggctttt ttaacgacgc caacgacgcg agccgtccac gcatcggccg
     6061 acatttccgc aacggtataa agattatctt gctgttgtaa atcggcaatt tgctgttcgc
     6121 caccgattaa gttgacctca taatatcccc agtcactgaa atgttctgta gcaagaatat
     6181 cggcatacac gccctgatgc gcacggtgaa atgcaccaaa gcctaaatga acaattcttg
     6241 gggccaggtt attacgatca taaacaggga gtgtcgcttt tgctgataac aaattatttc
     6301 ccataacaat tccttaaatt taaatatggc aagctacatg ttttgttata tgaagaaaaa
     6361 tcccctctcc ggtaagagaa gggattgagg atttaaagac ttctggaagg ttgcgcagct
     6421 cttacaacac gcggttgatc ttccgcagcg tcttccagcg cacttaaatc acggtctttc
     6481 acttctggca ttttcagcgc agagattaaa ccaatcactg aatatgccat gatcataatg
     6541 gcgattggat accaggattc cgtcatggtg cagaaaatac ccgccaggat aggaccaaaa
     6601 ccggaagcga taagaccacc aatttcttta gaaatagcca tccgggtaaa gcggttttta
     6661 cagccgaaca tttctgccat ggtaatgttt tccagagcaa ataatcccag caccgcacag
     6721 ttatgaatca caatcagtgc aaccataatg gtgctcgggg catagctttt atctacgatg
     6781 atagaaagca ttggccatgc cagcacaatc gcggaggtat tcataataat atacgggatc
     6841 cggcgaccaa ttttatcgga taaccaacca aggaacggaa tggtcataaa gccgagaatc
     6901 gaactaatca tcaatgcatc tgttggaatt gctttgttaa acaataacgt ctgcactaaa
     6961 tagcctgcaa ggaaagtctg aattaacccg gagttacccg cctgaccaaa acgcagccct
     7021 gttgccagcc agaaggattt gctctggaac atgctaccag caggtgcagg ttttgctgtc
     7081 ggttggttgc tgtcgttaac cttctcaaag accgggcttt ctttcagatt catacgcaac
     7141 cagatagcaa agaccatcac gacaacgctc gccaggaacg gtatacgcca tccccacgcc
     7201 agcagttcct ctttactgag aatgaagaac ataaaggccc agattgccgt tgcgcttaag
     7261 gttccgcagt tagttcccat agccacaaat gaggagataa ttccgcgctt accttttggc
     7321 gcatattccg ccagcatcgt accggcaccg gaaatttccg cacctgcacc caacccctga
     7381 ataatacgca gcgtcaccag caagatgggt gcaaaaacac caatctgtgc ataggtcggt
     7441 aacacaccaa ttaaggtggt acagatcccc atcatggtga tggtaataaa gagcactttt
     7501 ttacgcccta ttctgtcgcc cattttgccg aaaataaatg ctccgacaat acgcgccaca
     7561 taacctgcac cgtaggttcc cattgccaga attaacgcca ttgccgttga tgattcagga
     7621 aaaaatattt catgaaacac taacgctgcg ccgagtgaat ataactggaa atccataaat
     7681 tcaagcgcag tgcccagcca tcccgatact gctgctttca ccaaatcctt agtgcttctt
     7741 tcgtgttttt ctattgtcat aatggttatc tctaaaaaag aggtaagatg cgtactactt
     7801 actcgccgtt attggtatta ttcagaaaaa gtgagtaaga ctttgcagca atgtttttga
     7861 tcctgttcaa ataaactaat ggcatcagca acatgctgga aatcaaacgt atgggtaatt
     7921 aatttttctg gtttaattaa ccctttactt aaccagtcga taacgaccgg aaatttattt
     7981 gcatttaagc gtgaagagaa aatagagagt tcttttccgg taattccttg ctgaatcact
     8041 tcagacggtt cactggagaa gcccatcaat acaatacgtg ccgctggaga agccagcgtt
     8101 acggcctctt tcaggataga aggatgacaa gccgcatcaa taattaatgt cggctttatg
     8161 cctttttcag cgaaaatctc gccaagcggt gtctggctgt tattaatcac ccagtcagcc
     8221 ccgctctctt tcgctttttc cagtcgttca tcaatgcgat cggcaacaat cacattttta
     8281 acgttataga cgccttttaa tacctgaacg atcgtcaggc cgattggacc ggcaccataa
     8341 accagaacgg tatcattttc agtcggttga ccatgtccgg ttacgttagc cgcaatggta
     8401 aaaggttcga tcatcaccgc atattgatcg gccactgctt caggaatttt ccacgcattt
     8461 tttgccggaa ccacagcata ttcactgaaa ccaccgtcaa catgcacacc taatacagcg
     8521 agtgtcgtac aaacgttcgg cttacctata gagcacggat agcaatgacc acagctgacc
     8581 accggatcga cagcaacacg ttcaccgact ctggcgcttt ccacaccttc acccaccgca
     8641 tcaatgacgc caaagaattc atgtccaatg acgcgcggat atttcgcaaa aggattatgc
     8701 ccacgaaaaa tatggctatc tgaaccacaa attccggcaa gttttacttt tactcgaact
     8761 tcacccgctg acggggtggg tatttcacgt tcgataatcg ccagttgatt cggtttttca
     8821 attaaaatgc ttttcattaa cttactcctt accagttcca cagcgtgcca tcttccagac
     8881 gtgagactgg tagataagca ggttcatagg gatatttcgc cgccagcttt tcatcgaatt
     8941 cgatgccaag acccggtttg tctcccggat gcatatagcc gttatcgaaa gtccagttgt
     9001 gcgggaagac ttcgagcatt tgttcggaat aacccatgta ttcctggaca ccgaaattgg
     9061 ggacccacag atcaaagtgc agcgccgcag ccatgcagac tggtgacaaa tcggaaggac
     9121 cgtgtgagcc agtacgtacc tgatacaacg aagcaaaatc ggcaatccgg cgcataccgg
     9181 taattccgcc tgcatgggtc agcgtggtgc ggatataatc gataagttgc tcttcaatca
     9241 gttgcttgca gtcccagatg ctgttgaaga cttcacccac cgcgatgggt gtgatggtat
     9301 gttggcgaat gagacggaag cattcctggt tttccgcagg cgtcgggtct tccatccaga
     9361 acatgcgata atcttcaatg cttttaccaa agcgcgccgc ttcaataggc gttaagcgat
     9421 ggtgcatgtc atgcagcaaa tgttcattaa aaccaaactt gttacgtacc gcgtcaaaca
     9481 atttcggcat gaaatcgagg tatttctccg tcgaccacag ctgctcttcc ggccactgtc
     9541 ctttggttgc gggttcataa gccagacctt taccttccga catgccgtag gtggttttca
     9601 taccagggat gccgcactgc acgcggatgg ctttgaatcc cagctcctga tgacgggcat
     9661 aatcatccag agcttcatca atactgtgac cggtggtatg gcaataaacc atcacccctt
     9721 cacgagacgc gccgccgagt aactggtaaa gcggcatgtt ggcagctttg gctttaatat
     9781 cccacagcgc catatcaacc gctgaaatgg ccgacatcgt aaccggaccg cgacgccagt
     9841 aagcaccttt atagaaaaac tgccagatat cttcgatacg gtgcgcatcg cgaccaataa
     9901 gctgcggaca aaggtgatcc tgcaaataag aggccacaga aagctcacgt ccattgaggg
     9961 tggcatcccc aaggcccgta ataccgtcct cagtggtgat ttttaatgtg acgaaattac
    10021 gccccggaca ggtaacaaaa acttcagcct ttacgatctt catgttcgat tccttgcatc
    10081 gcttgtcgtg atgcatgaaa tctacgcaac tgagctacta ccatacaagt atgaagatca
    10141 aaaaaagccg gagtgatcac aaaaaaaggc gtatatttgc gctgtgaatg gttgacaaaa
    10201 gatgaaatag aatacctttt gtcagctgac acttcctcgt atcttattga taaaatggat
    10261 ttatgttcct acgtgcgccc ccagcccgca acaatgatca acatgccgca aagcgcaatc
    10321 aacgcacccg tccagtcata aagagtcagt ttcacgccat ccacaacgcg cagccacatc
    10381 aacgccgtgc agacataaac gccaccataa gccgcgtaaa cacgcccact cgccgcagga
    10441 tgcaacgtta acaaccagac aaacagcgcc agtgaaatcc ccgccggaag caacagccag
    10501 atactggcgt ttcgttttaa ccacaaccag ggcagaaagc atccaataat ttcacacagc
    10561 gcagtagcaa aaaatagtaa cgttgtttta atcatctttg tcacttattg acatcatgta
    10621 tagttatagg gcgacataat atcatcaata taaacaccct cctggtacgt tttgcgttcg
    10681 cagtggatgg tgtagaatca cctttaatca ttcatacagg gaatgaatta tgaaaatgac
    10741 tctcagcaaa cgaatcggcc tgctcgcttt tctgctgcct tgtgcactgg cattgagcac
    10801 aactgttcat gccgaaacta acaaactggt gattgagtct ggcgacagtg cacaaagccg
    10861 ccagcacgcc gctatggaaa aagagcaatg gaatgacacg cgcaatctgc gccagaaagt
    10921 gaataaacgc actgaaaaag agtgggataa agccgacgcc gcttttgata accgcgataa
    10981 atgcgagcaa agcgccaaca tcaatgccta ctgggagcca aatactttgc gctgcctgga
    11041 ccgtcgaact ggccgcgtta ttacccccta acctgttatt gatttaagga atgtaaggac
    11101 acgttatgcc aagcgcccac agtgttaagc tacgcccgct ggagcgtgaa gatttacgct
    11161 atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag gaaccctacg
    11221 aagcctttgt tgaactctct gatctgtatg ataagcatat tcacgatcag agcgaacgac
    11281 gctttgtggt ggaatgtgac ggcgaaaaag ccggtctggt ggagttggtg gaaattaacc
    11341 atgtacatcg ccgcgcagaa tttcagataa ttatctcccc ggagtatcag gggaaaggtc
    11401 tggcaacccg tgccgccaaa ttagcaatgg actatggctt caccgttctc aatctctata
    11461 agctgtatct gatcgtcgat aaagagaatg aaaaagcgat tcacatttac cgcaagcttg
    11521 gctttacggt tgaaggtgaa ttgatgcatg agttctttat taatggtcaa tatcgtaatg
    11581 ccattcgcat gtgtatattc cagcatcagt atctggcaga gcacaaaaca ccgggtcaga
    11641 ctctcctgaa gccgaccgca caatagcatt aataataatc gattgtattt ttgatggtgt
    11701 aaacccgttc gacggcgggt tttactcctt catcaacaat gattagctga cagtccaccg
    11761 gattggcgtg actgtcatat tcacagctct gttttacatt accaacccgt tgattgttca
    11821 gtaaagtaac cgctgtgtaa tctaattttt tgatcggatc cgttgatggc gtggcgctga
    11881 cagataatgt tttgtcgtta cttttcgtgg ttttacccag cggatacccc tgatcatcat
    11941 agcgatattc catctgcatc tgtttgctgc tggctttaat cacgaagcca ttatcatcgg
    12001 tttcccaact caccccggcg gaaggtaatt ccgctagctg gcattttccc tgtaaacgta
    12061 ctctcttctc cagcgtctca gcatcacggt aataattggc gtccagtacc agagcgacca
    12121 cggtattatt ttccagatcc agtaattcga gtgaatcaaa acagccttct tccgacaaag
    12181 tccccgaaac acgtttcgtc acttcacctt gctcatccat taatgtctga gtgaaatctt
    12241 ttaccggacc acgcagcgga tcaaaatcaa attcattaga gaaactggcc atctcaggag
    12301 taaatgaaag tgttacctct gtgcggtcac atcctgtgag taatatcgcg agtaagcatg
    12361 gcagtaattt gtatttcaca tcagtcacca gagagtagag atgattctca atcatagtag
    12421 caaatacagt actttacacg ttaaatgcta tgcttaaaaa agttatcttc gcgtaaggag
    12481 cttatgatga aactctcaac ctgctgtgcc gcgcttctgc tcgccctcgc ctcacccgcg
    12541 gtactcgccg cgccgggatc ctgtgagcgc atacagagcg atatatcaca gcgcattatc
    12601 aataatggcg tacccgaaag cagcttcacg ttaagtattg tacccaatga ccaggttgat
    12661 cagcctgatt cccaggtcgt cgggcattgt gctaatgata cgcataaaat tctctacacc
    12721 cgcaccacca gcggtaacgt ctctgctccc gcgcagtcta cccaggatgg cgcgcctgcc
    12781 gaaccgcagt aatacattat cgactgaacg ccggatacga cgcatccggc gatttgaaca
    12841 tataacataa cccaccttat tactcatacc cttctattga tatggattaa taattcttaa
    12901 cccaaaatgg gtagactccc tctattgtta gcgcgctaaa tattcaatat ataaactttt
    12961 atataacgat aaagaacagg gagtgagtta tgtccaaaaa tgaacaaatg gtgggcatca
    13021 gccgcagaac actcgttaaa tctaccgcga taggttctct ggcgctggct gcaggcggtt
    13081 tttctttgcc gtttaccctg cgcagtgcag cagcagcggt acaacaggcc cgcgaaaaag
    13141 tggtctgggg tgcctgttcc gtcaactgtg gtagccgctg tgcacttcgt ctacatgtta
    13201 aagataatga agtgacctgg gtggaaactg acaacaccgg cagcgatgag tatggcaatc
    13261 atcaagtacg cgcctgtttg cgcggtcgct ccatccgccg gcgtattaat catcccgatc
    13321 gcttgaatta cccaatgaaa cgcgtgggta aacgcggtga aggcaaattc gaacggatta
    13381 gctgggatga agccctggat actatcgcca gtagcctgaa aaaaaccgtc gaacaatatg
    13441 gcaatgaggc tgtatatatt cagtactctt cggggatcgt tggcggcaat atgacccgct
    13501 cttcgccatc agcctcggcg gtcaaacgcc tgatgaactg ctacggcggt tcactcaacc
    13561 agtatggctc ctacagcacc gcacaaattt cctgtgccat gccctacacc tacggcagta
    13621 atgatggcaa cagcaccacg gatattgaaa acagcaagct ggtcgtgatg tttggtaaca
    13681 acccggcaga aacccgcatg agcggcggtg gcatcactta tcttcttgaa aaagcgcgcg
    13741 agaaatcgaa cgccaaaatg attgttatcg atccgcgata taccgatacg gctgctggtc
    13801 gtgaggatga atggcttcct attcgcccgg gcaccgatgc cgcgctggtt gcggggattg
    13861 cctgggtatt gattaacgaa aatctcgttg atcaaccttt tctcgataaa tactgcgtcg
    13921 gctatgacga aataacctta cccgcagatg caccaaaaaa tggtcactat aaagcctata
    13981 ttcttggtga aggtgatgat aacacagcga aaacgccgca gtgggcttcg caaattaccg
    14041 gtatcccggt ggaccgtatc atcaaactgg cgcgtgaaat tggcacagca aaacccgctt
    14101 atatctgcca gggctggggg ccgcaacgcc aggcaaacgg cgagctgact gcacgcgcta
    14161 ttgctatgtt gcctattttg acgggcaatg tcggcatcag cggcggaaat agtggcgcgc
    14221 gtgaatcgac ctataccatt accatagagc gcctgccggt gctggataat ccggtcaaaa
    14281 cgtcaatctc ctgcttcagc tggacggatg ctatcgatca tggcccgcaa atgacggcaa
    14341 tccgcgacgg cgtccgcggc aaagataaac tggaagtgcc cattaagttc atctggaact
    14401 acgcgggaaa taccctggtt aatcagcatt ctgacatcaa caaaacgcat gaaattctgc
    14461 aagacgaatc gaaatgcgaa atgattgtgg tcatcgaaaa ctttatgacc tcatcggcaa
    14521 aatatgccga cattctgctg ccagacctga tgaccgttga gcaggaagat attattccta
    14581 acgactacgc cggtaacatg ggatatctca ttttcctcca gcctgtcacc agcgaaaaat
    14641 tcgaacgcaa accgatttac tggatcctga gtgaagtcgc gaaacgtctt ggacctgacg
    14701 tctatcaaaa gttcacagaa ggtcgcacgc aggaacaatg gttacaacat ctgtacgcca
    14761 aaatgcttgc caaagatccg gcgttaccgt cttacgacga actgaaaaaa atgggtatct
    14821 ataagcgtaa agatcccaat ggtcattttg tcgcctacaa agcatttcgt gacgaccccg
    14881 aggcaaatcc acttaaaaca ccttccggta agattgaaat ttattccagc aagctggcgg
    14941 aaattgcccg tacctgggaa ctggaaaaag atgaagtgat aagcccactg ccagtttatg
    15001 cctcaacctt tgaaggctgg gactcccctg aacgtagtac ctttccactg caactgtttg
    15061 gtttccatta caaatcccgt actcactcga cctacggcaa cattgatgtc ctgaaggctg
    15121 cctgccgtca ggaggtgtgg atcaacccta tagatgcgca gaaacgtggg attgccaacg
    15181 gcgatatggt gcgggtgttt aaccatcgtg gcgaagttcg gctaccagcc aaagtgacac
    15241 cacgtattct ccctggagtt agcgctatgg gccagggagc ctggcacgag gccaatatgt
    15301 ctggcgataa aatcgaccat ggaggctgtg tgaatacgct aaccactctg cgcccttcac
    15361 cactggcgaa gggaaacccg cagcatacga atctggtcga gatcgaaaaa atataaccca
    15421 ctacaaccat aaattctggc atgacatttt gtttgaaaag caataagtga gtaatgatga
    15481 aaatcgatac cacagaggcg ctcatgaagg ctgaaatcag ccgtagaagt ctgatgaaaa
    15541 cctccgcact tggcagtctt gcgctggcaa gcagtgcttt cactctgcca ttttcccaaa
    15601 tggtccgggc ggcagaggct ccggtagaag agaaagcggt ctggagttcc tgcaccgtta
    15661 actgcgggag ccgctgtctg ttacgtttgc atgtgaaaga tgacaccgtg tactgggtgg
    15721 agtctgatac gacaggtgac gacgtctacg gtaatcatca ggttcgagcg tgtttacgcg
    15781 ggcgctctat ccgccgacgg atgaatcatc ctgataggtt gaaatatccc atgaagcgcg
    15841 tcggcaagcg cggtgaaggt aaatttgaac ggataagttg ggacgaagcc ctggatacca
    15901 tcagtgataa tcttcggcgg atcctgaaag attacggcaa tgaggctgta catgtcctgt
    15961 acggaacagg tgtagatggc ggaaacatca ccaactcaaa cgtcccgtac cgtctgatga
    16021 actcttgcgg tggttttctc agtcgctatg gcagctacag taccgcacag atcagtgccg
    16081 caatgagtta tatgttcggt gccaatgatg gcaacagccc ggatgatatc gccaatacga
    16141 aactggtcgt tatgttcgga aataacccgg cagaaacgcg gatgagcggt ggtggtgtca
    16201 cttactacgt cgagcaagcc cgcgaacgtt caaacgcacg catgatcgtc atcgatccac
    16261 gttataacga cacagctgcc gggcgtgaag atgaatggct gcccattcgc cctggcaccg
    16321 atggcgcact ggcctgtgcg attgcctggg tgctgattac tgaaaacatg gtcgatcagc
    16381 catttctcga caaatattgt gttggttacg atgaaaaaac gctgcccgcc aacgcaccac
    16441 gtaacgcgca ttataaagcc tatattctgg gcgaagggcc tgacggcata gctaaaacgc
    16501 cggaatgggc agcaaaaatc accagcattc cggcagaaaa aattatccag ttggcacgag
    16561 agatcggttc agcaaaacct gcttatattt gtcagggttg ggggccacaa cgacactcca
    16621 acggcgaaca aacatcccgc gctattgcca tgctttccgt tctcaccggt aacgtcggca
    16681 taaacggcgg caactcaggc gtacgcgaag gtagctggga tctgggggta gaatggttcc
    16741 cgatgctcga gaatcctgtt aaaacgcaga tttccgtctt tacatggaca gatgccatcg
    16801 accatggtac ggaaatgacc gcgacacgtg atggtgttcg tggaaaagaa aaactggatg
    16861 tccccatcaa gtttttatgg tgctacgcca gtaacacatt gatcaatcaa catggcgaca
    16921 tcaatcacac ccatgaggtg cttcaggatg acagcaagtg cgagatgatt gttggcattg
    16981 accacttcat gacggcctcg gctaagtatt gcgatatcct gttgcccgac ctgatgccaa
    17041 cagagcaaga agaccttatc tctcatgaat ctgcagggaa tatgggctat gtgatcctcg
    17101 cccaacccgc aacctcagca aaatttgaac gcaaacccat ctactggatg ctgagtgaag
    17161 tcgccaaacg cttaggaccg gacgtttatc aaacctttac tgaaggtcgc agtcagcatg
    17221 aatggatcaa atatctccat gcgaaaacga aggaacgtaa ccctgagatg cccgactacg
    17281 aggagatgaa aacgaccggg atctttaaga aaaaatgccc ggaagaacac tacgtcgctt
    17341 tccgcgcatt ccgtgaagat ccacaggcaa acccgttgaa aacaccttcg gggaaaatcg
    17401 aaatttattc tgaacgactg gcgaagattg cagatacctg ggaattgaaa aaagatgaaa
    17461 ttattcatcc ccttcctgcg tataccccag gttttgatgg ctgggacgat cccctgcgga
    17521 aaacctatcc actgcagtta acgggcttcc actacaaagc gcgtacccac tccagctacg
    17581 gcaatattga tgtgttacag caggcctgcc cacaagaggt gtggatcaac cccattgatg
    17641 ctcaggctcg cggtatccgt catggcgata ccgtgcgggt atttaacaat aatggagaaa
    17701 tgctgattgc cgcaaaagtg actccgcgta ttctgcctgg cgttaccgcc atcgggcaag
    17761 gtgcgtggct taaagcggat atgtttggtg accgggtcga tcacggcggc agtattaata
    17821 ttctgacctc tcaccgccct tcaccgctgg caaagggaaa cccgtcgcac agcaatcttg
    17881 tccagatcga aaaggtttaa ggagtagccc atgaccacac aatatggatt ttttatcgat
    17941 tccagccgct gtactggctg taaaacctgc gaactggcgt gcaaagattt taaagatctt
    18001 ggcccggaag tcagtttccg ccgtatttat gaatatgctg gcggcgactg gcaggaagat
    18061 aatggcgtct ggcaccagaa cgtgttcgct tattatctct ccatctcctg taaccattgc
    18121 gatgaccccg catgtacaaa agtctgcccc agcggcgcaa tgcataagcg tgaagatggc
    18181 tttgtggtgg ttgatgaaga tgtttgtatc ggctgccgct actgccacat ggcttgtccg
    18241 tacggcgcgc cacagtacaa tgctgaaaaa gggcacatga cgaagtgcga tggttgttat
    18301 tcgcgcgtcg ccgaggggaa acaacccata tgtgtcgaat cctgcccgct gcgtgcactt
    18361 gagttcggtc caattgaaga gctgcgccag aagcacggca ctcttgctgc cgtcgcgcca
    18421 ctgccgcgcg cgcatttcac aaaacccaat atcgttatca aacctaacgc caacagccgc
    18481 ccgaccggcg ataccactgg ttatctggct aatccggagg aagtgtaaaa tgggaaatgg
    18541 atggcatgaa tggccactgg ttatctttac cgtattgggt caatgcgtag taggcgcgct
    18601 gattgttagc ggaattggct ggttcgccgc caaaaatgac gcagaccgtc agcacattgt
    18661 tcgtggcatg tttttcctct ggctgttgat gggcatcggg tttattgcct ctgtcatgca
    18721 cctgggatcg ccactgcggg cgtttaactc gctgaatcga atcggagctt ctggtctgag
    18781 taacgagatt gctgccggtt ccatattttt tgccgttggc ggactatggt ggctggtggc
    18841 ggtcatcggt aaaatgccgc aagcgttggg gaaactctgg ttacttgtca gcatggcgct
    18901 cggcgtcatt ttcgtctgga tgatgacctg cgtgtatcaa atcgacactg tgcccacctg
    18961 gcataacggt tacactacgc tggctttctt cctgacggta ttgttgagtg gtccaatact
    19021 tgctgccgca attctgcggg cagcccgcgt tacatttaat accacaccat ttgccatcat
    19081 tagcgttctg gcgctgatcg cttgtgccgg ggtgattgtg ctgcaaggtt tgtctctgtc
    19141 gtctattcac tcatccgtgc aacaagccag cgcactggta ccagattacg cctccttaca
    19201 ggtctggcgt gtggtattgt tatgcgcagg tctaggatgc tggctttgtc cgctcatacg
    19261 tcgccgtgaa cctcacgttg ccggacttat tctggggtta atcctcattc ttggcggtga
    19321 gatgatcggg cgtgtgctct tttatggcct gcacatgacg gtaggaatgg ctattgcagg
    19381 ttaacaaatt ggtgcggggc aacccgcaca tttcaggatg ttaggaatga cccatttttc
    19441 acagcaagat aatttttctg tcgcggcgcg cgtgttgggc gcgctgtttt attacgctcc
    19501 agagagtgcg gaagccgcac ctctggttgc ggtactcacc cgtgacggct gggaaactca
    19561 gtggcctcta ccagaagcgt cattagcgcc gctggtgacg gcttttcaga cacagtgtga
    19621 agagactcac gcccaggcct ggcagcgttt gtttgtcggc ccgtgggcac tgccgtctcc
    19681 gccgtggggg tcggtctggc tggatcgcga atctgtgctg tttggcgatt caacattggc
    19741 acttcgtcag tggatgcgcg agaaaggcat tcagtttgaa atgaagcaaa acgaaccgga
    19801 agatcatttt ggttcactgc tgttgatggc tgcgtggttg gcggagaatg gtcgccagac
    19861 agaatgcgaa gaattgctgg catggcacct ttttccgtgg tcaacacgtt ttcttgatgt
    19921 ttttatcgaa aaagcagaac accctttcta ccgtgcactg ggtgaactgg ctcgcttaac
    19981 gctggcgcaa tggcaatcac aactgttaat tcctgtcgcg gttaaaccgc tgtttcgata
    20041 ggatcaccgt aatattgccg gatggtgacg attacgcgca atccggcaat aataggttac
    20101 agtgtcacgt ttttttatct cttaaagcac gcactgcttt tgcggctggc ctcttttgcc
    20161 gcaaaatagt cgcccgtgtt tcattgccca tttctgctca tgcatcatct acacatctat
    20221 ccggatctgc gcactatgtt ccgccgtctg cttatcgcaa cagtcgtcgg tattctcgcg
    20281 gcctttgccg ttgccgggtt tcgtcatgcg atgctgctac tggagtggtt gttcctcaat
    20341 aatgactcgg gcagtctggt caatgcagcg acaaatcttt ccccctggcg acggctgcta
    20401 actccggcgc tcggcggact ggcggcgggt ttgttgctga tgggctggca gaaatttacc
    20461 cagcaacgcc ctcatgcgcc gaccgattat atggaagcgt tgcaaaccga gggtcagttt
    20521 gattacgcag caagcctggt taaatcgctt gcctctctgc tggtagtaac cagcggcagt
    20581 gcaattggtc gcgaaggtgc gatgattctt ttagctgccc ttgccgcctc ctgttttgcc
    20641 caacgtttta cgccacgcca ggagtggaaa ttatggatcg cctgtggggc cgcggcggga
    20701 atggctgcgg cctatcgtgc cccgcttgct ggcagtttat ttatagccga agtgctgttt
    20761 ggcactatga tgttggcctc tctcggcccg gtgattattt ccgccgtcgt ggcattgctg
    20821 gttagcaatc tgattaatca tagcgacgcg ttactctaca gcgtacaatt ctcagtgacg
    20881 gttcaggctc gtgactatgc gctgattatc agtacaggtg tgctggcagg tctgtgcgga
    20941 ccactgttgt taacgttaat gaacgcctgt catcgtggat ttgtgagtct caaacttgcg
    21001 ccgccctggc aactggcact aggcgggttg atcgtgggtc tgctttccct gttcacacct
    21061 gcagtgtggg gcaacggcta tagcaccgta caatcctttt taaccgcccc gccactgtta
    21121 atgatcattg ccgggatctt cctctgtaaa ctgtgtgccg tgctggcgag tagcggttcc
    21181 ggcgcacccg gtggtgtctt tacaccgacg ctatttatcg gtcttgccat tggcatgttg
    21241 tatggtcgta gcctgggatt atggttccct gatggcgaag aaattacgct tttactcgga
    21301 ttgaccggga tggcgacact gttggcggca accacgcacg cgccgattat gtcgacgttg
    21361 atgatatgtg aaatgaccgg ggagtatcag ctactccccg gtttattgat tgcctgcgta
    21421 attgcgtcgg taatctcgcg gacgttacac cgtgactcta tctaccgcca gcacactgcg
    21481 cagcatagct aagcggatgt attgccccag ttcacgctgt tcggcgcgcg gcagataagg
    21541 cagttcacca atgagcggtg ccggaagttt tttaccgagc acatcaatga tttccgcata
    21601 atgcgccagt cctgggttga ttcggttagc cacccagcca atgagcggca gcccgtcgtt
    21661 ggcaatcgcc tgagctgtta gcagtgcatg gttaatgcaa ccttcctgaa taccgacaac
    21721 catcaacacc ggcagttgtt cctgcaccac ccattcagag agtggacgca aatcattcat
    21781 cagactacgc cagccgccag tcccttctac cacgacatga tcgacttttt cggtcaggtt
    21841 tgccaggccg tttgaaatga gggtgtaatt gattgggcaa ctgtgcgcca cgctactttc
    21901 ttcttcgctt aacgcgatag gattaactgc ttcataaggc agttcgatgg ttgaaacact
    21961 ctgcaacacc agggcatctt tattacgcag cccttcgggt gtctctttgc tccccttcgc
    22021 tacgggttta tatcccgcaa ccgtttttcc ctgggaggct aacgcttgta gcaatgcgcg
    22081 ggaaaccacc gttttcccta cagaagtgtc tgtaccggta ataaagaaac gcttcagcat
    22141 cactaactcc accgttatgc ttcacaaata taaaccagga aaataattaa ccttgaaagt
    22201 ctaagttatg ctttcctggc ccaaattgag atagcgcaaa ttttggtaga acagttaaaa
    22261 aatgttaacc ctgcaacaga cgaatcaaca aagaaccgtt atacatcgcg tcttttacca
    22321 gcgcagcccc tgccatcgtg ccctggttag aaaactgagt actctcaacg ctaatgtgct
    22381 gactatacgc aggaagggcc tgctgacgga tgctatctga gatgaccggg aagaggatat
    22441 ctgccgcttt acttaacggt gagccaatca gtattttttg tgggttaaat aaattcacca
    22501 tgatggcaag aatgcgccct acatgcgcgc ccaccccggt aatgatgtct tttgccagca
    22561 gatcgccgcg caatgccgcc tgacacaatg agtccacggt taacggttgc ccatgtaaca
    22621 tcgagctcat ggattgattg agacgcagct gtgccagctc aagaatactg tccacactgg
    22681 cgatggtttc gaggcagccg tggttcccgc aataacagcg tttaccatat gggtctacct
    22741 gtgtgtgacc tatttccacg aggctactgc tgcctgcgtg tagcagatga ccatcggtaa
    22801 tgacgcccgc ccccacgttg tgatcgataa ccacctgaat cacatcgcgc gccccgcgtg
    22861 aggcaccaaa caaggcctct gccatcgtcc atgcgctgat atcatgctgt atataaaccg
    22921 gaacgccggt atgctgctcc agagcctcgc cgagcggcat ctcttttaca tcctcgtaga
    22981 acggcatgcg atgtacaatg ccattttccg tatcaataat tcccggcagg gttatggcaa
    23041 tcgaagttag acgctcaagt tttttctggt ggcggataaa aaactgatcg atatgggaaa
    23101 tgatacgatc cagcaatggc gagtcatctt ttaacgccag ttcctgcgac tcttccacca
    23161 ccagtttgct gctcagatcg cgcagagcaa ggaaaatctc cccgcgacta atgcgcagag
    23221 aaagatagtg ccaggcttca gtttcaacca ccagccccac cgccggacgg ccacggttcc
    23281 ccgcttcttt gatttccagc tcttgcacca ggtgtgcttc gagcatctca cggacaattt
    23341 tagtgatact ggcaggagcc agctgcgcca gacgggaaag atcgatacgc gagactggac
    23401 caagctgatc aatcaggcga taaaccgcgc ccgcgttggt ctgctttatt tgatcaatgt
    23461 gcccaggctg gttttcagca accaccgcat actccctata ttttcgcgct ccgaaataat
    23521 ctgtaggcta tggtgaagca cttcaatacg tgtcgtcaaa tttttactta ggcatgtgat
    23581 taacagcaca tttttcgggc tttttcgctg aaatttccct cacctgagag cattcagcag
    23641 atgaatacga aagttacgcg cagctggact ttgttcatga tgtttcggcc agaccaacca
    23701 catttcagaa accgcatcct cttcagcaat cggcacccag cgcatttcgt tgagctgaac
    23761 acgtttaaat gacgcaggca aaattgaaac acccagaccg gcggaaacca gaccgatgat
    23821 ggtcattgcc tcgcccacct cctgagtgat gacgggcgtc aaatggtaac gtcgcatcag
    23881 cccgagaata tcgtcataca gccctgtccc gacgtgcgga tcaaaaaaga caaagggttc
    23941 tttcgccagt tcagccatcg ttacattcgg gttatttgcc aggggatgat cgtgcgggat
    24001 catcgccata agcggttcat ggacgatgac tgcgtgttca agcgtctccg gtaacgctgt
    24061 gttacgcagc aatcccatat ccagcgttcc ttcaatgagc ggagcgattt gctcgcgagt
    24121 gttcatttcg cgggtttgta aatggacatc gggataatct cggcgaaaca gcgataacgt
    24181 atcggacacc gcccgaataa aaggagccga cgaagtaaaa ccaatgcgca actcccccgc
    24241 ttcaccctga tgcagtcttt cagcgcgagc ggcagcgtca tccaccatag acaggatttg
    24301 ccgactatct gcaagaaact gttttcctgc tgccgtcagc aatacactgc gattggttcg
    24361 tgccagcagt cgggcaccaa tttgttgctc cagcgcctga atctgctgac ttagcggcgg
    24421 ttgcgaaata ttcaggcggg cagcggcgcg cccgaaatgc agctcttccg caacagcaac
    24481 aaagtaacgc agatgacgaa gttcaatatt catatttaaa acatcttatt tgagattatt
    24541 aatatattag acagaacaat tcgattttcc taacctatgt ataagcctga tccacaggca
    24601 tatttagcaa ggatttcaag tgagccgtac tacaactgtt gatggcgctc cggcaagcga
    24661 cactgacaag caaagcattt ctcagccaaa tcaatttatt aaacgcggta cgccgcaatt
    24721 tatgcgcgtc accctggcgc tgttctctgc cggactggca acatttgcac ttctctattg
    24781 tgtgcagcct atccttccgg tgctttcgca ggagtttggc ttaacgcccg cgaacagtag
    24841 tatttcactg tccatttcca cggcgatgtt ggctattggt ttgctgttta ctggccccct
    24901 atccgatgcc attggtcgca aaccagtgat ggtcacggcg ctactgttgg cctccatttg
    24961 tacgttactt tcgacaatga tgaccagctg gcacggcatt ttgattatgc gcgccttgat
    25021 tgggctttcg ttaagtggcg tggcagctgt tggcatgact tatcttagcg aggaaatcca
    25081 ccccagtttc gtggcctttt cgatggggtt gtatatcagc ggcaactcaa ttggcggcat
    25141 gagcggacgc ttaattagcg gtgtcttcac ggactttttc aactggcgaa ttgctctggc
    25201 ggctatcggt tgtttcgcgc tagcctcggc gttgatgttc tggaaaatcc tcccggaatc
    25261 acgccatttt cgcccaactt cgctgcgacc taagtcgttg tttatcaact ttcgtctgca
    25321 ctggcgtgac cggggattac cgttattgtt cgcagaaggc tttttgctga tggggtcgtt
    25381 cgtcacgctg tttaattaca tcggctatcg gttgatgctc tccccctggc atgtcagtca
    25441 ggccgtggtt ggcttattat cgctggctta tttgaccggt acatggagct cacccaaagc
    25501 cggaaccatg accacccgct atgggcgtgg tccagtgatg ttgttttcga cgggggttat
    25561 gctgtttggt ttactgatga ccttattcag ctcgctgtgg ctgatctttg ccggaatgtt
    25621 actcttctca gcaggattct tcgcagccca ctcagttgcc agcagctgga tcggcccccg
    25681 cgcaaaacgc gctaaaggcc aggcctcctc gctgtatctg ttcagttact atctggggtc
    25741 gagtattgca gggacgctgg gtggtgtttt ctggcataac tatggctgga acggcgtcgg
    25801 cgcatttatt gctctgatgc tggtcattgc tctgctggtc gggacgcgtt tgcatcgtcg
    25861 tctgcacgcc tgaaaaataa gtccggactg cggtaaatac ccgtccggac ttattgcctg
    25921 ctcaaaccaa cgttaatagc catcctaaaa tagacgaagc gccagccaat tcccgcagcg
    25981 cgtctagcgt catcaggatt ataagtaccc aaataaacgg attcattttg ctgtgtgtca
    26041 tttattactg atgcgcagtt attctaccgc tttgtaagta gtaaaatagt taacccgatc
    26101 aagactacta ttattggtag ctaaatttcc cttaagtcac aatacgttat tatcaacgct
    26161 gtaatttatt cagcgtttgt acatatcgtt acacgctgaa accaaccact cacggaagtc
    26221 tgccattccc agggatatag ttatttcaac ggccccgcag tggggttaaa tgaaaaaaca
    26281 aattgagggt atgacaatga aaaaagtatt agctctggtt gttgccgctg ctatgggtct
    26341 gtcttctgcc gcctttgctg cagagactgc gaccacacct gctccgactg cgacgaccac
    26401 caaagcagcg ccggcgaaaa ctacacatca taaaaaacag cataaagcag cacctgccca
    26461 gaaagcgcag gcggctaaaa agcatcataa aaatgcgaaa gctgaacaga aagcccctga
    26521 acaaaaagcg caggcagcga agaaacacgc caagaaacac agccatcagc aaccggcaaa
    26581 acctgctgca caacccgcag cgtaagtttt caacagtaat gctggcgcgc cccctcgcgc
    26641 ctgaaaatta cggtgctaag cgggtaacgt ttagcaccgc ctttagcgga gggtagtatg
    26701 gtgggccgtt atcgctttga gttcattctg atcatcctta ttttatgcgc actgattgcc
    26761 gcccgttttt atctttcctg actgtagtta tctgatttta ctcccacttt catcccgtcc
    26821 cgtctatagt atttatgagg gtttgctttt aataatcata attacccacc agagtgtgat
    26881 atgcgtacaa ccattgctgt agtgttgggt gcaattagtt tgacgtctgc ttttgtgttt
    26941 gcagataaac cagacgttgc caaatcggca aacgatgagg tcagcaccct gttttttggt
    27001 catgatgatc gtgtgccagt gaatgacacg acccaatcac cgtgggatgc ggttgggcaa
    27061 ctggaaacgg ccagcggcaa tttatgtacg gcgacgctga ttgcacccaa tctggcatta
    27121 acggcagggc actgtttatt gacgccacca aagggtaaag ccgataaagc ggtggcgctg
    27181 cgttttgtgt caaataaagg tctttggcgc tatgagatcc acgacataga aggccgcgtt
    27241 gatccgacac tgggaaagcg gttaaaagca gatggggatg gttggattgt tcctcccgca
    27301 gctgcgccgt gggacttcgg tttgattgtg ctacgtaatc ccccttctgg cattacaccg
    27361 ttgccgttat ttgagggaga taaagccgcg cttactgccg cattaaaatc ggctggtcgt
    27421 aaagtgactc aggcaggata ccctgaagat catctcgata cgttgtacag tcatcaaaac
    27481 tgtgaagtga ctggctgggc gcaaacgtcg gtgatgtcac atcagtgcga taccttgccg
    27541 ggtgacagcg gttcgcctct gatgttgcat accgatgacg gctggcaatt aattggggtg
    27601 caaagttcgg ctcctgccgc gaaagatcgc tggcgcgccg ataaccgggc catttctgtt
    27661 accggttttc gcgacaagct ggatcaactg tcgcaaaaat aatgttcaaa cgctgcccga
    27721 cagcgcgggc agcgtcttca tcaggcaagt ttcaccatga tcattccagc caacagcaag
    27781 accaggccaa tccagccttt acgatttaac cgttgaccaa acaagatcca acctgcggct
    27841 aacgtggtgg caataccaaa cccgccccac aatgcataag cgacagacaa gtcgatccct
    27901 ttaacggcct gagaaagcgc actaaaggca gccagcaccg ccgcaaggga gagcaagcca
    27961 aatattttgc gacgaaagcc gtcagaaaat ttcaaaaaga cgttagcaac gatttccagc
    28021 acgattgcca atgccagcca ggcggcgtga acccattcaa actgcgccat ggttcacctc
    28081 ctgttcaggt ttacgcgctt tacgggtacc tgatttgatc aacactatcc cggcgaccag
    28141 ggtggttaac ccggcaattt tcatcagcga taaactttcg tcgaataaca aaacgctaaa
    28201 caaggtaata aataaaatac cgataccttc ccacagcgca taagccacgc ctaaggctat
    28261 ttttttaaca gcgaaagaga gaaatatata cgacagagaa atcatcacca gcattaaaat
    28321 aaagccgcca tttccctcac tgacgctcgc ccatttcata gacagcgtac cggtaatt
//
LOCUS       DAZFFG010000058        28352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFFG010000058 DAZFFG010000000
VERSION     DAZFFG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.058"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..290)
                     /locus_tag="ACHJ7J_002772"
     CDS             complement(<1..290)
                     /locus_tag="ACHJ7J_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5000987.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAA"
     gene            complement(361..2475)
                     /gene="fusA"
                     /locus_tag="ACHJ7J_002773"
     CDS             complement(361..2475)
                     /gene="fusA"
                     /locus_tag="ACHJ7J_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO5000988.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2572..3042)
                     /gene="rpsG"
                     /locus_tag="ACHJ7J_002774"
     CDS             complement(2572..3042)
                     /gene="rpsG"
                     /locus_tag="ACHJ7J_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO5000989.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3139..3513)
                     /gene="rpsL"
                     /locus_tag="ACHJ7J_002775"
     CDS             complement(3139..3513)
                     /gene="rpsL"
                     /locus_tag="ACHJ7J_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO5000990.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3639..3926)
                     /gene="tusB"
                     /locus_tag="ACHJ7J_002776"
     CDS             complement(3639..3926)
                     /gene="tusB"
                     /locus_tag="ACHJ7J_002776"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO5000991.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3934..4293)
                     /gene="tusC"
                     /locus_tag="ACHJ7J_002777"
     CDS             complement(3934..4293)
                     /gene="tusC"
                     /locus_tag="ACHJ7J_002777"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO5000992.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELASYDVILRF"
     gene            complement(4293..4679)
                     /gene="tusD"
                     /locus_tag="ACHJ7J_002778"
     CDS             complement(4293..4679)
                     /gene="tusD"
                     /locus_tag="ACHJ7J_002778"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO5000993.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4679..5401)
                     /gene="yheO"
                     /locus_tag="ACHJ7J_002779"
     CDS             complement(4679..5401)
                     /gene="yheO"
                     /locus_tag="ACHJ7J_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5000994.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5568..6380)
                     /gene="fkpA"
                     /locus_tag="ACHJ7J_002780"
     CDS             complement(5568..6380)
                     /gene="fkpA"
                     /locus_tag="ACHJ7J_002780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5000995.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6601..6819
                     /gene="slyX"
                     /locus_tag="ACHJ7J_002781"
     CDS             6601..6819
                     /gene="slyX"
                     /locus_tag="ACHJ7J_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO5000996.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6868..7458)
                     /gene="slyD"
                     /locus_tag="ACHJ7J_002782"
     CDS             complement(6868..7458)
                     /gene="slyD"
                     /locus_tag="ACHJ7J_002782"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5000997.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7553..7753)
                     /gene="yheV"
                     /locus_tag="ACHJ7J_002783"
     CDS             complement(7553..7753)
                     /gene="yheV"
                     /locus_tag="ACHJ7J_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO5000998.1"
                     /translation="MAIRKRFIAGAKCPVCQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7763..9568)
                     /gene="kefB"
                     /locus_tag="ACHJ7J_002784"
     CDS             complement(7763..9568)
                     /gene="kefB"
                     /locus_tag="ACHJ7J_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO5000999.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHTDT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9568..10119)
                     /gene="kefG"
                     /locus_tag="ACHJ7J_002785"
     CDS             complement(9568..10119)
                     /gene="kefG"
                     /locus_tag="ACHJ7J_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO5001000.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10250..12163
                     /gene="yheS"
                     /locus_tag="ACHJ7J_002786"
     CDS             10250..12163
                     /gene="yheS"
                     /locus_tag="ACHJ7J_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5001001.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12163..13185
                     /gene="yheT"
                     /locus_tag="ACHJ7J_002787"
     CDS             12163..13185
                     /gene="yheT"
                     /locus_tag="ACHJ7J_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5001002.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYTDAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13179..13397
                     /gene="yheU"
                     /locus_tag="ACHJ7J_002788"
     CDS             13179..13397
                     /gene="yheU"
                     /locus_tag="ACHJ7J_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO5001003.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13451..14320
                     /gene="prkB"
                     /locus_tag="ACHJ7J_002789"
     CDS             13451..14320
                     /gene="prkB"
                     /locus_tag="ACHJ7J_002789"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO5001004.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14375..14779)
                     /gene="yhfA"
                     /locus_tag="ACHJ7J_002790"
     CDS             complement(14375..14779)
                     /gene="yhfA"
                     /locus_tag="ACHJ7J_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO5001005.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15081..15713
                     /gene="crp"
                     /locus_tag="ACHJ7J_002791"
     CDS             15081..15713
                     /gene="crp"
                     /locus_tag="ACHJ7J_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO5001006.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15764..17854
                     /gene="yhfK"
                     /locus_tag="ACHJ7J_002792"
     CDS             15764..17854
                     /gene="yhfK"
                     /locus_tag="ACHJ7J_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO5001007.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17921..19141)
                     /gene="argD"
                     /locus_tag="ACHJ7J_002793"
                     /gene_synonym="dapC"
     CDS             complement(17921..19141)
                     /gene="argD"
                     /locus_tag="ACHJ7J_002793"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO5001008.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVMAAMPEFLQGLRELCDQ
                     YQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19227..19790)
                     /gene="pabA"
                     /locus_tag="ACHJ7J_002794"
     CDS             complement(19227..19790)
                     /gene="pabA"
                     /locus_tag="ACHJ7J_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO5001009.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19822..20424)
                     /gene="fic"
                     /locus_tag="ACHJ7J_002795"
     CDS             complement(19822..20424)
                     /gene="fic"
                     /locus_tag="ACHJ7J_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO5001010.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDFTALQMIFSKVVSEAGESE"
     gene            complement(20414..20581)
                     /gene="yhfG"
                     /locus_tag="ACHJ7J_002796"
     CDS             complement(20414..20581)
                     /gene="yhfG"
                     /locus_tag="ACHJ7J_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO5001011.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20686..21258)
                     /gene="ppiA"
                     /locus_tag="ACHJ7J_002797"
                     /gene_synonym="rotA"
     CDS             complement(20686..21258)
                     /gene="ppiA"
                     /locus_tag="ACHJ7J_002797"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO5001012.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21529..22710
                     /gene="tsgA"
                     /locus_tag="ACHJ7J_002798"
     CDS             21529..22710
                     /gene="tsgA"
                     /locus_tag="ACHJ7J_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO5001013.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22972..25515
                     /gene="nirB"
                     /locus_tag="ACHJ7J_002799"
     CDS             22972..25515
                     /gene="nirB"
                     /locus_tag="ACHJ7J_002799"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO5001014.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25512..25838
                     /gene="nirD"
                     /locus_tag="ACHJ7J_002800"
     CDS             25512..25838
                     /gene="nirD"
                     /locus_tag="ACHJ7J_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO5001015.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25964..26770
                     /gene="nirC"
                     /locus_tag="ACHJ7J_002801"
     CDS             25964..26770
                     /gene="nirC"
                     /locus_tag="ACHJ7J_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO5001016.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26789..28162
                     /gene="cysG"
                     /locus_tag="ACHJ7J_002802"
     CDS             26789..28162
                     /gene="cysG"
                     /locus_tag="ACHJ7J_002802"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO5001017.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6860 a         7599 c         7155 g         6738 t
ORIGIN      
        1 gcagcaccgg tgatcatgtt tttaacatag tcggcgtgcc ccgggcagtc tacgtgtgcg
       61 tagtgacggg tcggggtgtc atattcaacg tgagaagtgt tgatggtgat accacgagct
      121 ttttcttccg gcgcgttatc gatctggtcg aatgcacgag cagcaccgcc gtaggtttta
      181 gccagtacgg tggtgattgc agcggtcaga gtagttttac cgtggtcaac gtggccgata
      241 gtaccaacgt taacgtgcgg ttttgtacgt tcaaattttt ctttagacac ggctatattc
      301 cttactatag tgctctcccc ttcaggagag agcacgggac tttggtatta acccttaggc
      361 ttatttacca cgggcttcaa ttacggcctg agcaacgtta ctcggcgctt catcatactt
      421 caggaattcc atagtgtatg atgcacgacc tttggtcaga gaacgcagct gagttgcgta
      481 tccgaacatt tcagacagcg gtacttcagc gtggatctta acgccagtaa cttcagattc
      541 ctgacctttg agcataccac gacgacggct caagtcaccg ataacgtcac cggtgttctc
      601 ttccggagtt tctacttcaa ccttcatgat cggctcaagc agaactggtt tcgctttctt
      661 aaagccttct ttaaaggcga tagaagcagc cagtttaaac gccagttcag aggagtcaac
      721 gtcatggtaa gaaccgaagt gcagacgaat acccatgtct actaccgggt agcctgccag
      781 cggacctgct ttcagctgtt cctggatacc tttatcaacg gccgggatgt attcgccagg
      841 gattacacca cctttaatgt cgttgatgaa ctcgtagcct ttcgggtttg aacccggctc
      901 cagcgggtac atgtcgataa caacatgacc atactgacca cgaccaccag actgtttcgc
      961 gtgtttacct tcaacatcgg taactttctg gcggatagtt tcacggtaag caacctgcgg
     1021 tttacctacg ttcgcttcaa cgttgaattc acgcttcata cggtcaacga tgatgtcgag
     1081 gtgcagttcg cccatacccg caatgatggt ctggttagat tcttcgtcag tccatacacg
     1141 gaaagacggg tcttctttag ccagacggcc cagagccaga cccatttttt cctggtcagc
     1201 tttggttttc ggttcaactg cgatggagat taccggctca gggaattcca tacgctccag
     1261 aatgatcggc gcatccgggt cacacagggt gtcaccagtg gttacgtctt tcagaccgat
     1321 agcagcagcg atgtcgcccg cgcgaacttc tttgatctct tcacgtttgt tagcgtgcat
     1381 ctgaacgata cgaccgaaac gctcacgtgc agctttcacg gagttcagta cggtatcacc
     1441 agagttaacc acaccggagt aaacacggaa gaaggtcagg ttaccaacaa acgggtcggt
     1501 agcaattttg aacgccagtg cagagaacgg ctcgtcatca cttgcgtgac gttcagccgg
     1561 agtgtcttta ccgtcgtcca ggataccgtt gatcgcaggt acgtcaaccg gggatggcag
     1621 gtaatcaatt accgcatcca gcatcgcctg aacacctttg ttcttgaacg cagaaccaca
     1681 ggttaccagg atgatttcgt tgttcagaac gcgctgacgc agagcacctt tgatttctgc
     1741 ttcagtcagt tcctcaccac ccaggtattt ttccatcagc tcttcagaag cttcagctgc
     1801 ggattcgatc aggttctggt gccattcgtt agccagttca accatgtctg ccgggatatc
     1861 ttcgtattcg aaggttacgc cctggtcagc gtcgttccag ttgatagctt tcattttcac
     1921 caggtcaaca acaccggtga aatgttcttc agcaccaatc gccagctgca gcggaaccgg
     1981 gttcgcgccc agacgggttt tgatctggtt aacaactttc aggaagttcg cacccatgcg
     2041 gtccattttg ttaacgaacg caatgcgcgg aactttatat ttgtttgcct gacgccatac
     2101 ggtttcagac tgcggctgaa caccaccaac tgcgcagtaa accattaccg caccatcgag
     2161 cacacgcatg gaacgttcta cttcgattgt gaagtcaacg tgccccgggg tgtcgatgat
     2221 gttgatgcga tgcggctcat actgcttagc cataccagac cagaatgcag tagtcgcagc
     2281 ggaagtgatg gtaataccac gttcctgctc ctgctccatc cagtccatgg ttgcagcgcc
     2341 gtcatgaact tcaccgattt tatggtttac accggtgtag aacagaatac gttcggtagt
     2401 agtggtttta ccggcgtcga tgtgcgcact gataccgatg ttacggtagc gtgcgatggg
     2461 tgttgtacga gccatttgtt tcctcgttta tcttttaggc gttcaattta agtagcccaa
     2521 agcgggctgc ttactggaag cgcccgcctg gtgactaaaa ctccgaaggg attaccaacg
     2581 gtagtgtgcg aacgccttgt tggcttcggc catacggtga acgtcttcac gtttcttaac
     2641 tgcagtacct ttgttttctg cagcatcaga aagttcgttc gccaggcgca gagccatgga
     2701 tttatcaccg cgtttacgag cagcttcaac gatccaacgc attgccagag cattacgacg
     2761 aaccggacgg acttcaactg gtacctgata agtagaacca ccaacgcggc gagacttaac
     2821 ttctacagtc gggcgcacgt tttcgagagc tacttcgaat gcttccagtt cagatttacc
     2881 agagcgctga gccagggtct ccagcgcgct gtatacgata gattcagcag tagatttttt
     2941 accatctacc atcaggatat ttacaaattt agccagcagt tctgatccga acttcggatc
     3001 cggcagaatt ttacgctgac caatgacgcg acgacgtggc atggaaatac tccgttgtta
     3061 attcaggatt gtccaaaact ctacgagttt agtttgacat ttaagttaaa acgtttggcc
     3121 ttacttaacg gagaaccatt aagccttagg acgcttcacg ccatacttgg aacgagcctg
     3181 cttacggtct ttaacgccgg agcagtcaag cgcaccacgt acggtgtggt aacgaacacc
     3241 cgggaggtct ttaacacgac cgccacggat caggatcacg gagtgctcct gcaggttgtg
     3301 accttcacca ccgatgtagg aagtcacttc gaaaccgtta gtcagacgaa cacggcatac
     3361 tttacgcagc gcggagttcg gttttttagg agtggtagta tatacacgag tacatacgcc
     3421 acgtttttgc gggcatgctt ccagcgcagg cacgttgctt ttcgcaactt tgcgagcacg
     3481 tggtttgcgt accagctggt taactgttgc cattaaatag ctcctggttt tagcttttgc
     3541 ttcgtaaaca cgtaataaaa cgtcctcaca caatatgagg acgccgaatt ttagggcgat
     3601 gccgaaaagg tgtcaagaaa tatacaacaa tcccgccatc accaagccat ctggctggag
     3661 tgcttaaccg taagtctgac gaaatcagta tagtcaatgg gaatgatgtc gttcgaaatt
     3721 tgaccagtca aaccgcgggc aataaggtct tcgttcaggg catagacctt aatgggggca
     3781 ttacgcagac tttcaaggta gcggttaccg tcaactgcgg cagttacgcc atcttgcaat
     3841 aatagcagtt cgtcgccttc actgagcaga cgcagcagcg cagcaaaatc cgtcagccag
     3901 ggtgaacgat gtaacgtgtg cagcataaac gcctcaaaac ctcaaaataa catcgtagct
     3961 ggcgagctcg cggcgtaagg catctgcttc gagcggcgtg gcttcgacaa caaagggtgt
     4021 ctgcggatct aacccgcgtt cgcgcagtga agccgcacaa acccagcact gttcaatgtc
     4081 gtacagaccc aacaatttaa aagtggcaat gtaatcacgc gccagcactg catcgggctt
     4141 ttgtcctggc agtagctgaa aaacgccatc agcaataaag aagacggcca gctcgtcagt
     4201 taacgcggaa gttgccagta aagcatctaa accttcccgc ccggcagcgg taccatgagg
     4261 cacagtagaa aaaacaaacg caattcgttt catcagaact gtaccaccct gtcacaggtc
     4321 agcgaggctt ccgccagcgc gccaagtccg cttaaggtaa atcccggctg aaggtttgac
     4381 gaagccagcc ccagtcttcc ggcctccgtt tcatcaacaa cgccacggcg taatgctgcc
     4441 gctacgcaga tattcagcgc cacaccatgt tgcgcattca actgttgcca gccgcgcaca
     4501 aggtcaaatt cgtcgcttgc cggtgaggtc agttggttag cgttatagac cccttcccga
     4561 tagaaaaaga cgctgcttaa cttatggcct tctgctatca gcgcctgcgc aaactgaaaa
     4621 gcactactcg cctgttgcgt accgtatgct ggcccggtca ccacgatggc aaaacgcatt
     4681 acttatcttg cccctggaaa tcaccgctct tgaactggcg gatgtagaga tagacagtgt
     4741 gtttggagat gttcaggcgg tcagcaacct ggttgatcgc atctttaata tcgaagatcc
     4801 ctttctcgta gagattcagc acgatctgac gatttttggc gttattagaa acattgcgat
     4861 cggcattcac ttcttcgatg gtgaactcca gcgtttgggt aaccagatct tcaacagaag
     4921 aggcaaagtt gacgcttgaa ccgacatccg gggtttctgg cggcacaaag gtgctcataa
     4981 tctgcgagaa gggaacatca agattcatat tgatgcacag cagaccaatt acacgctgtt
     5041 cgcggttacg aatcgcgata gtcagggact tcattaatac gccgcttttg gcgcgagtaa
     5101 agtagcattt agaaacgctg ctatccgctc ccgtcatatc gtgcagcata cgtagcgcca
     5161 ggtcagtaat tggcgaacca atcttccggc ctgtatgttc accgttagca atgcgaatgg
     5221 ctgaacattt tagatcctgc aaagagtgca aaacgatttc acagtgggag ccaataagca
     5281 tcgctaaccc gtccaccacc gcttcgtagg atttcagaat atcaaaatcg gtctgctcga
     5341 aaggacgttg atccagtaaa tccaactcac tggtttcgtt ggttaaaagc gacctggaca
     5401 tgaaaaaaaa cactcctttt caggagcctg tcgttaactt ttcagggcag gctcattaat
     5461 gatgcgggta actaaattaa tacagcggag gttccgcttt ccagcactaa ttatatccgg
     5521 cctgtaataa aaaaaccgcc gcctggtcag gcggcggttc ttaatgctta ttttttagca
     5581 gaatctgcgg ctttcgcatc agcttccggc tttgcatcag ccttcggcgc tggtttcaca
     5641 tccagcagct ctacgtcaaa caccagggta gaattcggtg ggatccccgg aacacccgct
     5701 ttgccgtaag ccagttctgg tggaataacc agtttgatct taccgccttt cttgatgttc
     5761 ttcagacctt ctgtccaacc cgggataaca ccgtccagac ggaaagaaag cggttcacca
     5821 cgggtgtaag agttgtcgaa ctctttaccg tcgatcagcg tacctttgta gttcactaca
     5881 acagtatcgc tgtctttcgg tgcttcgcct ttaccggctt ctactacctg ataaaccaga
     5941 ccagttgaag aggttttcac acctttctct ttggcaaatt tctcgcggta ctctttacct
     6001 tttgcttcgt tatcagccgc gtctttttcc atcttcgcct gagcagaaga cttcacgcga
     6061 gcttcgaatg cttgcagagt ctgttcgatc tcttggtcgg agagtttgct cttatcagca
     6121 aatgcatcct gaacaccagc gatcagctga tctttatcca gtttgatgcc cagtttttct
     6181 tgttctttta gagagttttc catgtaacga cccagcgagg cacccagtgc ataagctgat
     6241 ttctgatcgt catttttgaa cgctgctttg ctgtcagcag ttgtagcagg ttttgcagct
     6301 tcagcagcaa aagtgattgg tgcatgcaga gcaacggcca ttgtggtcgc cagcagcgtt
     6361 actttaaaca gtgatttcat ccatatctcc aggatcgggg catctcaccc cagggttaac
     6421 tattatcaga agggtactat aaagcgttgt cgaacaaatc tacatacaga cacgccctat
     6481 tatcatctat tttcagactc tttttgttta aattagtttc gatgaccgcg aaatgagtgc
     6541 tgtctcgggc agcaaagtta agtagaatcc gcggcgacca ttcgacaaaa gaggtgaatc
     6601 atgcaggatt tatcattgga agcacgcctg gcagagctgg agagccgact ggcttttcag
     6661 gaaatcacca ttgaagaact gaacgtcacg gtgaccgctc atgaaatgga gatggcgaaa
     6721 ctgcgcgatc atctgcgtct gctgaccgag aagttaaaag ccagccagcc gtcgaacatc
     6781 gcgtcgcagg ctgaagaaac gccaccgcca cattattgag gcgtaaaaaa agcggggatt
     6841 ccccgctttt ttgtcacttt ttcggtatta gtggcaaccg caaccgccgt tacctttacc
     6901 gccacagcag ccttcgccac cgtgctcatg accgtgatca tggccatgac caccgcagca
     6961 accgtcgtgg tcgtgatcgt ggtgatgatc gtgcgcaccg tgaacgtggc catgagccag
     7021 ctcttcttca gtcgcttcgc gaatcgccac aacttcaacg ttgaatttca ggttctgacc
     7081 ggccagcatg tggttaccat caaccacgac gtgatcgtct tcaaccgcag tgatttcaac
     7141 cggtaccgga ccctggtcgg tttcagccag gaaacgcata cctacctgca gttcatcaac
     7201 gcccataaat acgtctttag gcacacgttg caccaggttt tcgtcgtact gaccgtaagc
     7261 gtcgttcgcg ccaacagcga catcaaattt gtcgccaact tcatgacctt ccagcgccgt
     7321 ttccaggcca gagatcaggg aaccgtgacc atgcaggtag tccagcggcg cactcaccgg
     7381 agactcatca accaacacac cgtcttctgt acgtacctga taggccaggc tgaccaccag
     7441 gtcttttgct actttcatga tatctcctga gcatgggaag aatagtggcg cagattgtag
     7501 cggaattctg cagccgtgta ctcactagct taaaaaaact cggcgcatat cgctagtccg
     7561 gatgaaaaat cccgatcact tgctcatctt tgcgaacgtg atcgcgagct tctttgtctg
     7621 cttctcgcat ctgatgtccg cacttaacac attcaacaat atcaatatta ttttcgcgcc
     7681 acatcgccat tgaatcctgc gcctgacaga ccgggcattt tgcgcccgca ataaaacgtt
     7741 ttcggattgc catctttacc ctctactcaa attcatccca gccgtccagc tggcgtcgtt
     7801 cttgttgcat ttcacgctgg aaaatctctt ccagttcgcg tcgggcttcc ctggcgcgag
     7861 aaatttgtac ggtatcggta tgcattggga tgagctctcg cagcatccgc atatccaggc
     7921 ggcgaaaatg cagttgcgcc cgctgtgcct gatgcggatg cataccaagc gtgaccagcg
     7981 tcttgcgccc cagctctaac gcactggaga atgtttcacg ggaaaactgc gtcacccctg
     8041 cctgtaataa ctcatgcgct tccacacgtc cgcgcgctcg cgcaagaata tgcaaatgcg
     8101 gaaagtgctg ctggcatatt tccaccagct tcatggtgtc ttccggctcg ttacaggtaa
     8161 tgacgataga ctcagcggcc tctgcacccg cagaacgtaa aagatctacc tgcgtggcgt
     8221 cgccgtaata aactttgtag ccatatttgc gcatcaggtt aacggcgctg atatcccgct
     8281 ccagcacagt aatgcgcatt ttatttgcca tcaacaaacg accaatcacc tgcccaaaac
     8341 gtccaaagcc cacgacaatg acctggggtt tatcatcgtt gacccacggt ttttcgtctt
     8401 cttcttccgg tccgttaaac tggcgggata gccatttatc caccagcttc atcagcagcg
     8461 gcgtggtcat catggaaagc gtcaccgtca ccagcaacag cgccatctgg tcgccctgga
     8521 ataagcgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8581 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8641 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8701 gggtataaag caccccgagg ttgagtgaca tcccgacaga gataaagaac aaaccgagca
     8761 gcaagccttt aaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8821 gcagcacgcc tgctataaac gtaccgagcg ccatcgacag ccccagcgcg tccataaaca
     8881 atgcggaacc caataccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8941 ctgcaataaa acggaatacc ggacgcaata aatagcgccc gccaatcagc atgccgacaa
     9001 acgccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaacccgcca
     9061 acaacggcac taacgccagt gccgggatca ccgccagatc ctgaaacagc agtaccgaaa
     9121 acccgagctg cccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9181 ttgcggtcga agacatcgca aggccaatgc caccgaccac cgccgcctgc caggcgaaat
     9241 ccgtcagcat caataatccc gccagcaacg ctgcgcttaa cagcacctgt gccgcgccta
     9301 cgccaaaaat cgaacgccgc agttgccaaa gcttggaggg attcaactca aggccgatga
     9361 taaacatcag gaacaccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9421 taaaccccag cccccacggg ccaattgcaa tccctgccag caaatatccc aacacagcgc
     9481 caatacccag ccgcgatgcc aacggcaccg cagccaccgc cgcgaagaga aacagcactc
     9541 ctgcaagtaa aaaatcggaa ccttccatca gcggcctcct ggagacagcg gatttgccag
     9601 ccagtcaccg taggctctgg cgtggctcgc cagctcctgt gcgctttgcc gtctcgccca
     9661 ataaataatg atgggactta accaatgcat ccggcacatg cccgccgcca gttcaaaggg
     9721 gcgcaggaca tcgctcatcg ggtagcgatt caacgcgtca taacggtaag cactttccgg
     9781 ctcgccggtg gtaatcacac tacgccagta ctttcccgcc agttggtttc ctcccggccc
     9841 gctggcaaaa ccacggctta atacccggtc cagccactct ttcagtagcg ccgggcagct
     9901 ataggtataa agaggatgct gaaagacaat cacctcgtgc tcgcgcagta atgcctgctc
     9961 acgggggata tcaataaaaa aatcgggata gtgcgcgtaa aggtcgtgca cggtaacatt
    10021 gctgagctgc gtggccggtt taagcagtac ccggtttgcc accgagtcct gagattccgg
    10081 atgggcatac agcagcaaaa ctttcgctgg ctgagacatc atccccctcc cggtatggtt
    10141 tttgtgtata gtcgctgttt tgggctacca ttgcgcccgg tgcggcagct cgcccataca
    10201 ttacattatc ataatgataa gttaacatag tctgaacata cggcacctta tgattgtttt
    10261 ctcctcgtta caaattcgtc gcggcgtgcg cgtcctgctg gataatgcca ccgccaccat
    10321 caaccccggg cagaaagtcg gcctggtggg taaaaacggc tgtggtaaat ctaccctgct
    10381 ggcattgctg aaaaatgaaa tcagcgccga cggcggcagc tacacctttc cgggaagctg
    10441 gcaactggcg tgggtgaatc aggaaacgcc ggcgttaccg caagcggcgc tggaatatgt
    10501 cattgacggc gaccgtgaat atcgtcaact ggaagcgcag ctacacgacg ccaacgaacg
    10561 taacgacggg cacgccattg cgaccattca tggcaagctg gatgctattg acgcatggag
    10621 tattcgctcc cgtgccgcca gcctgctgca cggcctcggt ttcagcaatg aacaactgga
    10681 gcgcccggta agtgattttt ccgggggctg gcgtatgcgt cttaaccttg cccaggcgct
    10741 gatttgccgt tcagacttgc tgctgctcga cgaaccgact aaccacctcg atctcgatgc
    10801 cgttatctgg ctggaaaaat ggctgaagag ctatcagggc acgctgatcc tgatctctca
    10861 cgaccgcgac ttcctcgatc cgatcgtcga taaaattatt catatcgaac aacaaagcat
    10921 gttcgagtac accggcaact acagttcgtt tgaagtacag cgcgccaccc gtctggcgca
    10981 gcaacaagcg atgtacgaaa gccagcagga acgcgtagcg catctgcaaa gttatatcga
    11041 ccgtttccgt gccaaagcca ccaaagcgaa gcaggcccag agccgcatta agatgctcga
    11101 gcgtatggag ctgattgccc cggcgcacgt cgacaatccg ttccgcttta gcttccgcgc
    11161 gccggaaagt ctgccaaatc cgttactgaa gatggaaaag gttagcgcag gctatggcga
    11221 tcgcattatt ctcgactcga ttaaactgaa tctggtcccc ggttcgcgca ttggtctgtt
    11281 aggccgcaac ggcgcgggta aatcgacatt aatcaaactg ttagccggtg aacttgcgcc
    11341 agtcagtggt gaaataggcc tggcaaaagg gatcaagctc ggctacttcg cccagcatca
    11401 acttgaatac ctgcgcgccg acgaatcgcc tattcaacat ctggcacgtt tagcgccgca
    11461 ggaactggag caaaaactgc gtgactacct cggcggcttt ggtttccagg gcgataaagt
    11521 aaccgaagaa acgcgccgct tctccggtgg ggaaaaagcc cgcctggtgc tggcattaat
    11581 cgtctggcag cgtccgaatc tgctgctgct cgacgaaccg actaaccacc ttgaccttga
    11641 tatgcgtcag gcactcaccg aagcattaat cgagtttgaa ggcgcgctgg ttgtcgtttc
    11701 gcacgaccgt catttgctgc gttccaccac tgacgacctc tacctggttc acgatcgtaa
    11761 agtcgaaccg ttcgacggcg atctggaaga ttatcaacag tggttgagcg acgtacaaaa
    11821 gcaggaaaac cagaccgacg aagcgccaaa agagaacgcg aacagcgccc aggcacgtaa
    11881 agatcagaag cgtcgggaag cggagttgcg tgcgcaaacc cagccactgc gtaaagagat
    11941 tgcccgtctg gaaaaagaga tggagaagct gaacgcgcaa ctggcgcagg cagaagagaa
    12001 actcggcgac agcgaactgt atgaccagag ccgtaaagcg gagttgaccg cctgcctgca
    12061 acagcaagcc agcgccaaat ccggcctgga agagtgcgaa atggcatggc tggaagccca
    12121 ggagcagctt gagcagatgt tgctggaagg ccaaagcaac tgatggcgca gataacgacg
    12181 accgatgcca atgaattcag cagcagtgct gaattcaccc ctatgcgcgg ctttagcaat
    12241 tgtcatctgc aaaccatgct gccgcgtctg tttcgtcgca aggtgaaatt caccccgtac
    12301 tggcagcggc tggagttgcc cgacggcgat tttgtcgatc tcgcgtggag tgaagcccca
    12361 gcacaggcgc ggcataaacc gcgtttagtg gtatttcacg ggctggaagg cagtctcaat
    12421 agcccttacg cccacggtct ggttgaggcg gcgcaaaagc gcggctggct gggcgtggtg
    12481 atgcattttc gcggatgcag cggtgaacca aaccgtatgc accgcattta ccattcgggc
    12541 gaaaccgaag acgccagttg gtttttacgc tggctgcaac gcgaatttgg tcatgcgcca
    12601 acggctgccg tcggctattc gctcggcggt aatatgctgg cctgtttgct ggcaaaagaa
    12661 ggcaatgatc tcccggttga tgcggcggtg attgtctctg cgccgtttat gctggaggcc
    12721 tgtagttacc atatggaaaa gggcttttcc cgcgtttatc agcgttactt gctgaacctg
    12781 ttaaaagcca atgccgcacg caagctggct gcctaccccg gaacgctgcc gataaatctc
    12841 gcgcaattaa aatcggtacg tcgcatccgt gaatttgacg atctaatcac cgccaggatt
    12901 cacggctaca ctgacgctat cgactattat cgtcagtgta gcgccatgcc gatgctgaac
    12961 cggatcgcca aaccgacgct gattattcac gccaaagacg atccgtttat ggatcatcag
    13021 gtgatcccga aaccggaaag tctccccccg caggtggagt atcaactgac tgaacatggc
    13081 ggtcatgttg gctttattgg cggtacatta cttcatccgc aaatgtggct ggagtcacgc
    13141 attcctgact ggttaacaac gtatctggag gcgaaatcat gttgattccg tggcaagacc
    13201 tctcccccga aacgctggaa aatttaattg aaagctttgt gttacgtgaa ggcaccgatt
    13261 atggtgaaca tgagcgtaca cttgaacaga aagtcgccga cgtcaaacgc cagctacagt
    13321 gcggagaagc ggtgctggta tggtcggagc tgcacgaaac ggtcaatatc atgccgcgca
    13381 gtcagtttcg cgaataacct gcaaccgtcg gcggacgaga taataatgat agctaagtca
    13441 tggagttact atgtctgcca aacatccggt cattgcggta acaggatcca gcggcgcggg
    13501 gaccaccacc accagcctcg cgtttcgtaa aatattcgcg cagttaaatc tgcatgcagc
    13561 cgaggtggaa ggcgacagtt ttcaccgtta cacccgcccg gaaatggaca tggcgatccg
    13621 caaagcgcgc gacgccgggc ggcatatcag ctacttcggc cccgaagcga acgactttgg
    13681 cctgctggaa caaaccttca ttgaatatgg tcagagcggc aaagggaaat ctcgcaaata
    13741 tctgcatacc tacgacgaag ccgtaccctg gaatcaggta ccgggaacat tcaccccctg
    13801 gcaaccttta ccagaaccca ctgatgtact gttttatgaa ggtttacacg gcggcgtagt
    13861 cacgccacag cataacgttg cgcagcatgt ggacttactg gtcggcgtgg tgcctatcgt
    13921 taaccttgag tggattcaaa aactgatccg cgacaccagc gagcgcgggc actcacgaga
    13981 agcagtgatg gattcagtag tgcgttcaat ggaagactat atcaactaca tcacaccgca
    14041 gttttcccgc acccatctta acttccagcg cgttcccacc gtcgacactt caaacccgtt
    14101 cgcggcaaaa ggtatcccgt cgctggatga aagctttgtg gtgatccatt ttcgtaatct
    14161 ggaagggatc gatttcccct ggctgctggc gatgttgcaa ggctcattca tttcccacat
    14221 caatacgtta gtggtgcccg gcggcaaaat gggtctggca atggaattaa ttatgctgcc
    14281 gctggtgcaa cgattgatgg aaggaaagaa aatcgagtaa ctctgctatt acgccggata
    14341 aaatactatc cggcttcaca acgggatagt taaatcacgc ggcaaccact tcatacgagt
    14401 gagtaatatt caccgctttt tccagcatca acgccactga acaatatttc tcggcagaga
    14461 gatcaaccgc acgcgcaacc gctgcgtctt tcaggtcgcg accggtgacg ataaaatgca
    14521 gattaatgtg cgtaaacagg cgtggtgcct cttcgcggcg ttcagaggtc aattttactt
    14581 cacaatcgac cacatcctga cgcccttttt gcaggatcga aaccacatcg atggcactgc
    14641 aaccacccgc cgccatcaac accatttcca tcggacttgg tgctttatcg cctgagttgc
    14701 cgtccattaa aatctgatga ccagaggcgg attcgcccag aaaagttaac ccttcgaccc
    14761 acttcactcg cgcttgcatt tttgctactc cactgcgtca attttcctga cagagtacgc
    14821 gtactaacca aatcgcgcaa cggaaggcga cctgggtcat gctgaagcga gacaccagga
    14881 gacacaaagc gaaagctatg ctaaaacagt caggatgcta cagtaataca ttgatgtact
    14941 gcatgtatgc aaaggacgtc acattaccgt gcagtacagt tgatagcccc ttcccaggta
    15001 gcgggaagca tatttcggca atccagagac agcggcgtta tctggctctg gagaaagctt
    15061 ataacagagg ataaccgcgc atggtgcttg gcaaaccgca aacagacccg actctcgaat
    15121 ggttcttgtc tcattgccac attcataagt acccatccaa gagcacgctt attcaccagg
    15181 gtgaaaaagc ggaaacgctg tactacatcg ttaaaggctc tgtggcagtg ctgatcaaag
    15241 acgaagaggg taaagaaatg atcctctcct atctgaatca gggtgatttt attggcgaac
    15301 tgggcctgtt tgaagagggc caggaacgta gcgcatgggt acgtgcgaaa accgcctgtg
    15361 aagtggctga aatttcgtac aaaaaatttc gccaattgat tcaggtaaac ccggacattc
    15421 tgatgcgtct gtctgcacag atggcgcgtc gtctgcaagt cacttcagag aaagtgggca
    15481 acctggcttt cctcgacgtg acgggccgca ttgcacagac tctgctgaat ctggcaaaac
    15541 aaccagacgc tatgactcac ccggacggta tgcaaatcaa aattacccgt caggaaatcg
    15601 gtcagattgt cggctgttct cgtgaaaccg tgggacgcat tctgaagatg cttgaagatc
    15661 agaacctgat ctccgcacac ggtaaaacca tcgtcgttta cggcactcgt taatcccgtc
    15721 ggagtggcgc gttacctggt agcgcgccat tttgtttccc ccgatgtggc gcagactgat
    15781 ttatcacccc gatatcaact atgcactacg acaaacgctg gtgctatgtt tgcccgtggc
    15841 cgttgggtta atgcttggcg aattacgatt cggtctgctc ttctctctcg ttcctgcctg
    15901 ttgcaatatt gcgggccttg atacgcctca taaacgtttt ttcaaacgct taatcattgg
    15961 tgcgtcgctg tttgccacct gtagcttact gacacagctg ctactggcaa aagatgttcc
    16021 cctgcccttt ttgctgaccg gattaacgct ggtacttggc gtcactgctg agttggggcc
    16081 attgcacgca aaattgcttc ctgcatcgct gctcgccgcc atttttaccc tcagtctggc
    16141 gggatacatg ccggtctggg aaccgttgct catctatgcg ttgggcactc tctggtacgg
    16201 attgtttaac tggttttggt tctggatctg gcgcgaacaa ccgctgcgcg agtcactaag
    16261 tctgctgtac cgtgaactgg cagattattg tgaagccaaa tacagcctgc ttacccagca
    16321 caccgaccct gaaaaagcgc tgccgccgct gctggtgcgc cagcaaaaag cggtcgatct
    16381 aattacccag tgctatcagc aaatgcatat gctttccgcg caaaataata ctgactacaa
    16441 gcggatgctg cgtattttcc aggaggcgct ggatttacag gaacatattt cggtcagttt
    16501 gcatcagccg gaagaggtgc aaaagctggt cgagcgtagc catgcggaag aagttatccg
    16561 ctggaatgcg caaaccgtcg ccgctcgcct gcgcgtgctg gctgatgaca ttctttacca
    16621 tcgcctgccg acgcgtttta cgatggaaaa gcaaattggc gcactggaaa aaatcgcccg
    16681 ccagcatccg gataatccgg ttgggcaatt ctgctattgg cacttcagcc gcatcgcccg
    16741 cgtgctgcgc acccaaaaac cgctctatgc gcgtgactta ctggccgata aacagcggcg
    16801 aatgccgtta cttccggcgc tgaaaagtta tctgtcacta aagtctccgg cgctacgcaa
    16861 tgccggacga ctcagtgtga tgttaagcgt tgccagcctg atgggcaccg cgctgcatct
    16921 gccgaagtcg tactggatcc tgatgacggt attgctggtg acgcaaaatg gctatggtgc
    16981 aacccgtctg aggattgtga atcgctccgt gggaaccgtg gtcgggttaa tcattgcggg
    17041 cgtggcgctg cactttaaaa ttcccgaagg ttacaccctg accttgatgc tgattaccac
    17101 ccttgccagc tacctgatat tgcgcaaaaa ctacggctgg gcgacggtcg gttttactat
    17161 taccgcagtg tataccctgc aactgttgtg gctgaacggt gagcagtaca tccttccgcg
    17221 tcttatcgat accattattg gttgtttaat tgctttcggt ggtaccgtct ggctgtggcc
    17281 gcagtggcag agcgggttat tgcgtaaaaa cgcccatgac gctttagaag cctatcagga
    17341 agcgattcgc ttgattctga gcgaggatcc gcaacctacg ccactggcct ggcagagaat
    17401 gcgggtaaat caggcgcata acactctgta taactcattg aatcaggcga tgcaggaacc
    17461 ggcgtttaac agccattatc tggcagatat gaaactgtgg gtaacgcaca gccagtttat
    17521 tgttgagcat attaatgcca tgaccacgct ggcgcgggaa caccgggcat tgccacctga
    17581 actggcacaa gagtatttac agtcttgtga aatcgccatt cagcgttgtc agcagcgact
    17641 ggagtatgac gaaccgggta gttctggcga tgccaatatc atggatgcgc cggagatgca
    17701 gccgcacgaa ggcgcggcag gtacgctgga gcagcattta cagcgggtta ttggtcatct
    17761 gaacaccatg cacaccattt cgtcgatggc atggcgtcag cgaccgcatc acgggatttg
    17821 gctgagtcgc aagttgcggg attcgaaggc gtaatgcagg ctaaaccatc ggataaggca
    17881 ttcacgccgc atccgacatc ttttgcctga tgcgtcttat ttacgcccca accaccttcg
    17941 ccaccgcgtg ggcgaaacgt tgcatccctt catcgatatc cgcatcttcc accaccagcg
    18001 acggcgcaaa acgcatcaca tccggtccgg cattcagcac cattacgcca gcctctgcgc
    18061 ccgcatacag gaaatcacgc gcctgacctt tgtactgtgg tttcagctcc gcgccaatta
    18121 acagccccat accgcgaata tcgctaaaca catcgtactg ctgatcgatc ttctgcaaat
    18181 ggtcaacaaa acgctggcgt ttcgcctgaa tgccttccag cacttcaggg gtattgatga
    18241 tatcaaacgc cgcgcccgct actgcacagg ccagaggatt accgccgtag gtggaaccgt
    18301 gagaaccagg atgaaacgca gaagcaattt ccgccgtggt cagcatggcg ctaatcggga
    18361 agccgccgcc taacgctttc gcagaggtca gaatatccgg cgtcacaccg tagtgcatgt
    18421 aagcaaacaa atcaccggtc cgccccatcc cgcactgcac ttcatcaaac accaataatg
    18481 cctgatattg atcgcacagc tcgcgcaagc cctgcaaaaa ctctggcatc gctgccatca
    18541 caccgccctc gccctggatc ggctcaacca ccaccgcaca ggtgtgatca tccatcaccg
    18601 ctttcactgc gtggagatcg ttaaagggaa cgtggatgat gtctgctggt tttggcccaa
    18661 agccgtcgga atattttggc tgcccaccca ccgaaacggt aaacagcgag cgaccatgaa
    18721 aagcgttatg gaaggcaata attttggttt tgaacgggct atgacgcaca caggcgtaat
    18781 ggcgtgccag tttaaaggcg gtttcgttag cttccgtgcc agagttcata aacaccacac
    18841 gttcggcaaa cgttgcctca atcagtttac gcccaagacg cagcgccggt tcattggtga
    18901 aaacgttact gatatgccac agagtttcgc cctgggtttt taacgcgttc accagcgcag
    18961 gatggcaatg gcccaacgcc gtaactgcaa tgccacccgc gaaatcgaca tactccttgc
    19021 cttgctgatc ccagattcgg ctgccctgac cttttaccgg aatgaactct gccggagcat
    19081 aaatcggcag gatcacttca tcgaaagtcg cgcgtgtaat tgctgtttgt tcaattgcca
    19141 tctcatgatc accctgttac gcataaataa atgtgaaatt ataatcacaa aatatgcata
    19201 aaaaatcatt gaatggcaat cagaaatcag cgatgcagga aattagccag cagttgatgt
    19261 ccttgttcgc taagaatact ttctggatgg aactgcacac cttccagatc ccactggcga
    19321 tggcgaatcc ccataatctc gcgggtttcg ctccaggccg tcacttcaaa gcacgctggt
    19381 aacgagtcag gttccactac cagcgaatga tagcgtgtca cggtaagtgg atttgccagc
    19441 ccccgaaata cgccctcacc gttatgtgta atcggcgagg ttttgccgtg catgaccttt
    19501 gcggcgcgca caactttacc gccaaatgcc tgcgccattg cctgatgacc gaggcagacg
    19561 ccaagaatcg gcaagcgccc ggcatagtgg cgaataacgt caagggagat cccggcttca
    19621 tctggcgtac aggggccagg tgagatgaca attttttgtg gtttaagggc gtcgatatcc
    19681 gccagcgtca acgcatcgtt gcgcttaacc agcacatccg cccccagttc acaaaagtac
    19741 tggtagaggt tccaggtaaa agaatcgtag ttatctataa gcaggatcat ggcggctccg
    19801 gtacgaaaga accgcgctat tttactcaga ttccccggct tcgcttacca ctttgctaaa
    19861 tatcatctgc agtgcggtga aatcccccat tgccccactc tgatttgcct gattccaggc
    19921 ctctttttca ataccctgcc agctcagttg atatccggca tgaatcgcca gttgctcgaa
    19981 gaagatccgc tgtgccagac cacttcccac ccggaaggga tgcagcacgt tgatttcaca
    20041 atagtaatgc gccagccgct cgacgaactt cgctttctcc aggccaacca gataaccttc
    20101 ttcctccaga tcctgcatca gggcattgcc ctctttttcg atataagcaa agtggcagaa
    20161 cggcgtatcg ccctgataaa tatcaacttc acgcagttgc cctgcccagt cgaaaatatc
    20221 ctgatacagc tggcgatgga tagtgcgcaa atgcggtaaa ccgcgcacca gcggaccaag
    20281 ctcaatggtc gcagcacgca gcgccgtcat ttcgtaagcg gcctgttcca gccgttgttg
    20341 ctggcggatg ttcagccggt tacgcatgat atcaaggcca ggataaagat acggatcgcg
    20401 cccttcgccg aatttatcgc tcatagtgac tcctcagctc ttcaaggcgc gctaaagcct
    20461 ctgcggcagt aagagtgact aaaggcatct cgacgccttc aagacggcga ctggcctgaa
    20521 aattacgatt acgctgaagc tcccagagac gggacttttg cttatcggtg agtttcttca
    20581 cttgatgcct ccctgaacgt gtccatttgc cacaagtata agcggcaaat ccgggttacg
    20641 ccgggagagc agaagcgcgg gcaagattgc ccgcgcgaga aatcattacg gcaggacttt
    20701 agcggaaagg ataactaccg gttttgacgg cacattctgg tacggaccaa catcatgagt
    20761 cggcacctgg gaaatcttat cggcaacgtc catgcctttc accactttac caaataccgc
    20821 gtaaccgaaa tcacgctgac catggtcaag gaaggcgtta tcggcaacgt tgataaagaa
    20881 ctggctggtg gcgctgtctt tgtcagcggt acgcgccatc gcgatggtgc cacgcgtgtt
    20941 gcgcaggccg ttatcggctt catttttgat tggcgggttt ggttttttct gctgcatctg
    21001 ctcggtgaaa ccgccgccct gaatcataaa gccaggaatg acgcggtgaa aggtagtgtt
    21061 gttataaaaa ccgctgttca cataatcgac aaagttttgc acagacactg gcgctttttg
    21121 tttatccagc tccagttcga tgttaccagc tgaggttgtc aacaatacgt gcgggtcccc
    21181 tttcgctgcc attgctgcgg gagaaagagc agaaagagcg aaaacagcag ccatcgccgc
    21241 cagggtcgat ttgaacatga gatttcctta acaaacagag cagaaaaaaa gcaagtgaag
    21301 agattctaaa tagccttcaa gaacaaggcc atccctttac ctaattttac gtatctgaaa
    21361 tatatgtaac ctgacgatca ctttttattc cgtgatcaaa atcacctctt aaaatgcaat
    21421 ttagcaaccg attgcaataa aacatttaaa cagatcacaa aatcacctaa aatcgcccgc
    21481 tcgcggcata tcccgctatg ccattttatt tttgaacgca ggccaatcat gactaacagc
    21541 aatcgcatca agctcacatg gattagcttt ctctcctacg cactgactgg tgcgttggtt
    21601 attgtcaccg ggatggtgat gggaaatatc gccgattatt tcaatctgcc tgtttccagt
    21661 atgagtaata ccttcacctt cctcaacgcc ggcattttaa tctctatctt cctcaacgcc
    21721 tggctgatgg aaatcgtccc gttgaaaacg cagttacgtt ttggctttct cctgatggtg
    21781 ctggcggttg ccggtttgat gttcagccac agcctggcgc tgttctcggc ggcgatgttc
    21841 attctcgggg tggtcagcgg catcaccatg tcgattggta cattcctggt aacacaaatg
    21901 tatgaagggc gtcagcgcgg ttcccgcctg ttatttaccg actccttctt cagtatggct
    21961 gggatgattt tcccaatgat cgccgcgttt ctactggcgc gcagcattga gtggtactgg
    22021 gtttatgcct gcatcgggct ggtgtatgtc gctattttta ttctgacctt cggctgtgag
    22081 ttcccggcac tgggcaaaca tgcaccaaaa acggatgctc cggtagcgaa agaaaagtgg
    22141 gggatcggcg tactgtttct ctctgttgcg gcactgtgct acatcctcgg tcagttaggt
    22201 tttatctcct gggtgcctga gtatgccaaa ggcctgggca tgagcctgaa tgatgctggc
    22261 acgctggtga gtaacttctg gatgtcatac atggtcggca tgtgggcgtt cagctttatt
    22321 cttcgcttct ttgatttgca gcgcattctg accgtactgg ctggtctggc tgcgattctg
    22381 atgtacgtct ttaacaccgg aacaccggca catatggcgt ggtcaattct cgccctgggc
    22441 ttcttctcca gcgcgatcta taccaccatc atcaccctgg gttcacagca gaccaaagta
    22501 ccgtcgccaa aactggttaa ctttgtcctg acctgcggga ccatcggtac tatgttgacc
    22561 tttgtggtta ccggcccgat tgttgaacat agtggtccgc aggcggcgct gctgacggca
    22621 aacggtctgt acgctgtcgt ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac
    22681 cgtcagcata acaccctgac ctctcattaa ttgttcatgc cgggcggcac tatcgtcgtc
    22741 cggccttttc ctctcttact ctgctacgta catctatttc tataaatccg ttcaatttgt
    22801 ctgttttttg cacaaacatg aaatgtcaga caattccgtg acttaagaaa atttatacaa
    22861 atcagcaata taccccttaa ggagtatata aaggtgaatt tgatttacat caataagcgg
    22921 ggttgctgaa tcgttaaggt aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa
    22981 gtcagactcg caattatcgg taacggtatg gtcggccatc gctttatcga agatcttctt
    23041 gataaatctg atgcggccaa ctttgatatt accgttttct gtgaagaacc gcgcatcgct
    23101 tatgaccgcg tacacctctc gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg
    23161 gtgcgcgaag gcttctacga gaaacacggc atcaaagttc tggtcggcga acgcgctatc
    23221 accatcaacc gtcaggagaa ggtgattcac tccagcgccg ggcgcaccgt tttttatgac
    23281 aagctgatca tggcgaccgg ttcctacccg tggatcccgc caatcaaagg ttctgatact
    23341 caggactgct ttgtctatcg caccattgaa gacctcaacg ccattgaatc ctgcgctcgt
    23401 cgcagcaagc gcggtgccgt tgttggtggc ggcctgttag gtctggaagc tgctggcgcg
    23461 ctgaaaaact taggcattga aacccacgtt atcgaatttg cccctatgct gatggcggaa
    23521 cagcttgatc agatgggcgg cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc
    23581 gttcacacca gcaaaaacac ccttgagatt gtgcaggaag gtgttgaagc gcgtaaaacc
    23641 atgcgttttg ccgacggcag cgaactggaa gtcgacttta tcgtcttctc taccggtatc
    23701 cgtccgcgcg ataagctggc aacccagtgt ggtctggacg ttgctccgcg tgggggtatt
    23761 gtcattaatg attcctgcca gacttccgat ccggatatct acgccatcgg tgaatgcgca
    23821 agctggaaca accgtgtatt tggtctggta gcaccaggct acaaaatggc gcaggtcgcc
    23881 gttgaccata ttctcggtag cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg
    23941 aaactgctgg gcgtagacgt aggcggtatt ggtgatgcgc acggtcgcac accaggcgca
    24001 cgtagctacg tttacctcga cgaaagcaaa gagatctaca aacgccttat tgtcagcgaa
    24061 gacaacaaaa ccctgctcgg tgcggtactg gtgggcgata ccagcgacta cggcaacctg
    24121 ctgcaactgg tgctgaatgc tatcgagttg ccggaaaacc cggattctct gattctgccg
    24181 gcgcactcgg gtagcggtaa gccgtctatc ggtgttgata aactgccgga cagcgcgcaa
    24241 atctgctcct gcttcgacgt caccaaaggt gatctgattg ctgccatcaa caaaggctgc
    24301 cacaccgttg cggcgctgaa agccgaaacc aaagcgggta ctggctgcgg tggctgtatc
    24361 ccgctggtca ctcaggtact gaacgcggaa ctggcgaaac agggcatcga agttaacaac
    24421 aacctgtgcg aacactttgc ttattcgcgt caggaactgt tccatttgat ccgcgttgaa
    24481 ggcattaaaa ccttcgaaga actgctggcg aaacacggca aaggttacgg ttgtgaagtt
    24541 tgtaaaccga ccgtcggttc gctgctggcc tcctgctgga acgaatacat tctgaagccg
    24601 gaacatactc cgctgcagga ttctaacgat aacttcctcg ctaacatcca gaaagacggc
    24661 acctactcgg tgatcccgcg ctctccgggc ggtgaaatca ccccggaagg actgatggca
    24721 gtgggtcgta tcgcgcgtga atttaatctc tacaccaaaa tcaccggctc ccagcgcctg
    24781 gcgatgtttg gcgcacagaa agacgatctg ccggaaatct ggcgtcagct gattgaagcc
    24841 ggtttcgaaa ccggtcatgc ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt
    24901 agcacctggt gccgctacgg cgtgggcgac agcgtgggcc tcggcgtgga actggaaaac
    24961 cgctacaaag gcatccgtac gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt
    25021 gaatgttcag aagctcaggg taaagacgtg ggtattatcg ccaccgaaaa aggctggaac
    25081 ctgtatgttt gcggtaacgg cggcatgaaa ccgcgtcatg cggatctgct ggcggcggat
    25141 atcgatcgcg aaacgctgat caaatatctc gaccgcttca tgatgttcta catccgtacc
    25201 gccgacaaac tgacgcgtac cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac
    25261 ctgaaagcag tgatcattga cgacaaactg gggctgaacg cacatctgga agaagagatg
    25321 gcgcgcctgc gtgaagcggt agtgtgtgag tggactgaaa cggtcaatac gccgtctgcg
    25381 cagactcgct tcaaacactt catcaacagc gacaagcgtg acccgaacgt gcagatggtg
    25441 ccagagcgcg aacagcaccg tccggcaacg ccgtatgaac gtatcccagt aactctggtg
    25501 gaggacaacg catgagccag tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa
    25561 ccggcgtctg cgcgctgtta ggtgacgagc aggtcgcgat tttccgcccg tatcacagcg
    25621 atcaggtgtt tgcgatcagc aacatcgacc cgttcttcga gtcaagcgtg ctgtcacgcg
    25681 gactgattgc ggaacaccag ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt
    25741 ttcgcttaag cgacggcttg tgcatggaag acgaacagtt ttccgtcaaa cattacgaag
    25801 cgcgagtgaa agacggcgtg gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg
    25861 cctccccttt ttgcatggtc ctgtaataac cttcggtata ttgcaggaca ttttttaaac
    25921 tttttgtttt attttttgtt tttatttttt aaaggataat caaatgttta cagacactat
    25981 taataagtgt gcggctaacg ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg
    26041 cttttgggtc agctccgcca tggcgggcgc gtatgtgggt cttgggatca tcctgatttt
    26101 cacgctcggt aatttgctcg atccatccgt acgccctctg gtgatgggcg cgacctttgg
    26161 tatcgcttta acgctggtga ttatcgccgg ttctgaactg ttcaccggac acaccatgtt
    26221 cctcaccttt ggggtgaaag cgggcaccat cagccacggg caaatgtggg caatcctgcc
    26281 gcaaacctgg ctgggtaacc tggtcggttc cgtctttgtt gccatgctct atagctgggg
    26341 cggtggtagc ctgctgccgg tagataccag catcgttcac tccgtcgcat tggctaaaac
    26401 cactgcaccg gcaatggtac tgttcttcaa aggtgcattg tgtaactggc tggtttgtct
    26461 ggcaatctgg atggcgctgc gtactgaagg ggcggcgaaa tttatcgcaa tctggtggtg
    26521 tctgctggca tttatcgcgt ccggctacga gcactccatc gctaacatga cgctgttcgc
    26581 gctctcctgg ttcggcaacc acagcgaagc ctacacgctg gcgggtattg gtcataacct
    26641 gctgtgggtg acgctgggta atactttatc aggtgccgta ttcatgggat tgggttattg
    26701 gtatgctacg ccgaaagcga atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc
    26761 tgccggttaa ttactaaggg gtttttacgt ggatcatttg cctatatttt gccaattacg
    26821 cgatcgcgac tgtctgattg tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct
    26881 gttagacgca ggtgctcgct taacggtgaa tgcattagcg tttattccac agttcaccgc
    26941 atgggcagat gcaggcatgt taaccctcgt cgaagggcca tttgatgaaa gccttctcga
    27001 cacctgctgg ctggcgattg cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga
    27061 agccgctgaa gctcgtcgca tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt
    27121 tattatgccg tcgattattg accgctcacc gctaatggtc gcggtctcct ctggcgggac
    27181 ctctccggtt ctggcgcgcc tgttacgcga aaaacttgaa tcactgctgc cgttgcatct
    27241 gggccaggta gcgaaatacg ccgggcaatt acgcggtcga gtgaaacaac agttcgccac
    27301 gatgggtgag cgtcgccgtt tctgggagaa attgtttgtt aacgaccgcc tggcgcagtc
    27361 gctggcaaac aacgatcaga aagccattac tgaaacgacc gaacagttaa tcaacgaacc
    27421 gctcgaccat cgcggtgaag tggtgctggt tggtgcaggt ccgggcgatg ccggtctgct
    27481 gacactgaag ggtctgcaac aaattcagca ggcagatgtg gtggtctacg accgtctggt
    27541 ttctgacgat attatgaatc tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg
    27601 cgcgggatac cactgcgtac cgcaggaaga gattaaccag atcctgctgc gggaagcgca
    27661 aaaaggcaaa cgcgtggtgc ggctgaaagg tggcgatccg tttatttttg gccgtggtgg
    27721 cgaagagctg gaaacactgt gcaacgcggg tattccgttc tcggtggttc cgggtattac
    27781 cgcagcttct ggttgctctg cctattcggg tattccgctc acgcatcgcg attatgccca
    27841 gagcgtacgc ttaattaccg gacacttaaa aaccggtggc gagctggact gggaaaacct
    27901 ggcggcagaa aaacagacgc tggtgtttta tatggggctg aatcaggctg cgactattca
    27961 gcaaaagctg attgaacacg gtatgcctgg cgaaatgccg gtagcaattg tcgaaaacgg
    28021 aacagcagtc acgcagcgcg tgattgacgg tacgctcaca caactgggcg aactggcgca
    28081 gcaaatgaac agtccatcgc taattattat tggtcgggtt gttggcctgc gcgataaact
    28141 gaactggttc tccaaccatt aatttaatca gggccagaga attctggcct ttttcgttta
    28201 caactctctt tatatattca gcagataaaa atataactca ttaacaaaaa tcatttgttt
    28261 ttgccccgtt atattcaccg ttacagcgac ttacaaaatt aagtcattca ctcactgacg
    28321 ccgattaaat attcctgtat cgttcttaat ga
//
LOCUS       DAZFFG010000059        28344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFFG010000059 DAZFFG010000000
VERSION     DAZFFG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.059"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(48..989)
                     /gene="fabY"
                     /locus_tag="ACHJ7J_002803"
                     /gene_synonym="yiiD"
     CDS             complement(48..989)
                     /gene="fabY"
                     /locus_tag="ACHJ7J_002803"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO5001018.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1034..1471)
                     /gene="dtd"
                     /locus_tag="ACHJ7J_002804"
     CDS             complement(1034..1471)
                     /gene="dtd"
                     /locus_tag="ACHJ7J_002804"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO5001019.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1468..2340)
                     /gene="yihY"
                     /locus_tag="ACHJ7J_002805"
     CDS             complement(1468..2340)
                     /gene="yihY"
                     /locus_tag="ACHJ7J_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO5001020.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2334..2933)
                     /gene="yihX"
                     /locus_tag="ACHJ7J_002806"
     CDS             complement(2334..2933)
                     /gene="yihX"
                     /locus_tag="ACHJ7J_002806"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO5001021.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3074..3961)
                     /locus_tag="ACHJ7J_002807"
     CDS             complement(3074..3961)
                     /locus_tag="ACHJ7J_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5001022.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(3951..5300)
                     /locus_tag="ACHJ7J_002808"
     CDS             complement(3951..5300)
                     /locus_tag="ACHJ7J_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5001023.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(5399..6832)
                     /locus_tag="ACHJ7J_002809"
     CDS             complement(5399..6832)
                     /locus_tag="ACHJ7J_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5001024.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLIHWEPLGANVDAEDAEVLAELKK"
     gene            complement(6902..7912)
                     /locus_tag="ACHJ7J_002810"
     CDS             complement(6902..7912)
                     /locus_tag="ACHJ7J_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO5001025.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(7945..8832)
                     /locus_tag="ACHJ7J_002811"
     CDS             complement(7945..8832)
                     /locus_tag="ACHJ7J_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO5001026.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQIMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(8857..9735)
                     /gene="yihU"
                     /locus_tag="ACHJ7J_002812"
     CDS             complement(8857..9735)
                     /gene="yihU"
                     /locus_tag="ACHJ7J_002812"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO5001027.1"
                     /translation="MATIAFLGLGQMGSPMANNLLHKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            9908..10804
                     /locus_tag="ACHJ7J_002813"
     CDS             9908..10804
                     /locus_tag="ACHJ7J_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5001028.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIARLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            10844..11632
                     /locus_tag="ACHJ7J_002814"
     CDS             10844..11632
                     /locus_tag="ACHJ7J_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5001029.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(11743..13008)
                     /gene="yihN"
                     /locus_tag="ACHJ7J_002815"
     CDS             complement(11743..13008)
                     /gene="yihN"
                     /locus_tag="ACHJ7J_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5001030.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(13110..14090)
                     /gene="yihM"
                     /locus_tag="ACHJ7J_002816"
     CDS             complement(13110..14090)
                     /gene="yihM"
                     /locus_tag="ACHJ7J_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5001031.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(14098..14808)
                     /gene="yihL"
                     /locus_tag="ACHJ7J_002817"
     CDS             complement(14098..14808)
                     /gene="yihL"
                     /locus_tag="ACHJ7J_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5001032.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(15025..16848)
                     /gene="typA"
                     /locus_tag="ACHJ7J_002818"
     CDS             complement(15025..16848)
                     /gene="typA"
                     /locus_tag="ACHJ7J_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO5001033.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            17221..18630
                     /gene="glnA"
                     /locus_tag="ACHJ7J_002819"
     CDS             17221..18630
                     /gene="glnA"
                     /locus_tag="ACHJ7J_002819"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO5001034.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            18804..19853
                     /gene="glnL"
                     /locus_tag="ACHJ7J_002820"
     CDS             18804..19853
                     /gene="glnL"
                     /locus_tag="ACHJ7J_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO5001035.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            19865..21274
                     /gene="glnG"
                     /locus_tag="ACHJ7J_002821"
     CDS             19865..21274
                     /gene="glnG"
                     /locus_tag="ACHJ7J_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO5001036.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            21386..21496
                     /gene="yshB"
                     /locus_tag="ACHJ7J_002822"
     CDS             21386..21496
                     /gene="yshB"
                     /locus_tag="ACHJ7J_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO5001037.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(21686..23059)
                     /gene="hemN"
                     /locus_tag="ACHJ7J_002823"
     CDS             complement(21686..23059)
                     /gene="hemN"
                     /locus_tag="ACHJ7J_002823"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO5001038.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELALAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(23248..23754)
                     /gene="yihI"
                     /locus_tag="ACHJ7J_002824"
     CDS             complement(23248..23754)
                     /gene="yihI"
                     /locus_tag="ACHJ7J_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO5001039.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(23873..24039)
                     /locus_tag="ACHJ7J_002825"
                     /pseudo
     CDS             complement(23873..24039)
                     /locus_tag="ACHJ7J_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24336..24968
                     /gene="yihA"
                     /locus_tag="ACHJ7J_002826"
     CDS             24336..24968
                     /gene="yihA"
                     /locus_tag="ACHJ7J_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO5001040.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(25350..28136)
                     /gene="polA"
                     /locus_tag="ACHJ7J_002827"
     CDS             complement(25350..28136)
                     /gene="polA"
                     /locus_tag="ACHJ7J_002827"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO5001041.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7112 a         7384 c         6876 g         6972 t
ORIGIN      
        1 atataaatga cctgaatgag agaggcagca ccgcccctct ccatcagcta ctcttcttcg
       61 ttcccgccct cttcatacgg gccaaatggc ttcgcgggca gaacgatata cgtgccttca
      121 aacactgcac ccggcgtctc gtcgccaaag atttcgacct gcatctgcac ccgtgctttt
      181 cgtccgcgcg ccagacggtc gagatcgccg cttaaggcac caaggtcggc taccgcatgg
      241 ggtttaccgc taatcggttt gctgtagcgg atatgcgcat ccgccagaat aatcgttccg
      301 ccgaggtggc gttcacgcag catcagccag ataagccccc aaccggtgag cgtcgccagt
      361 gagaataaac tcccggcaaa cagcgtatgg tgcggattct gattgccggt ttctggcatg
      421 gtggtgataa atttttgccc ggtatattgt tgaatgcgca cgcccatttt ttcgcttaac
      481 gggatatgtt cgtaccacgc ctgttgcagc tgcgcgcacc agtcaccgcg atgcagaatg
      541 tcatccagag tggcaacggg tttaatcatc aaaaaatggc gaatcggcgt ggtagttggg
      601 gtggtgatct ccccctgatt aatgaatccc agcttggcga aaaactccac cgcgtcttca
      661 cgggcgctac aggtcacgcg cttaacgcct tcctgacgcg ccaccgactc cagggtcatc
      721 gccatcagcg tgcctaaccc tttgtcctgc acgtcgggat gaacggccat aaagcgaatg
      781 gacgcttcat tgtcggcatt aatatacagt cggcctaccg ccaccagatt accctgctcg
      841 tcgacgacca tctggtgatg cgccatcgca tcccacgcgt cgcgttccga accttttggt
      901 tgatgcaggg gcttacgcaa catttcccag cgaaactgat agtaacgctc taactcttct
      961 tctgtttgtg gaacccgaag gtgatacata gcgatactct ctcttgtttc ccgtgacaac
     1021 cctggaagct ggctcatacc tgcaaccaga atgtcacggg gccatcattg accagcgata
     1081 cctgcatatc cgcagcgaag cgtcctgttt gcgtgttcat ctcttgctga cggcagcgtt
     1141 cgacgaaata gtcatataac gcctctgcgc gatccggtga tgcacctttg gagaaacttg
     1201 ggcgcatccc ccgttcggta tctgcggcga gggtaaactg ggaaaccacc agcacactgc
     1261 cgcccgcctg ttgcacgttg agattcatct tgccttcggc atcgctaaag atgcggtagc
     1321 cgagcacacg ctcgcacaga cggtttgctt tctgttcgtc gtcatccttt tcgacaccca
     1381 ataacaccaa aagtcccgcg ccaatttcgc ccgtcacttc tccctccacg gtgacgctgg
     1441 cacgggttac gcgttgaatt aatgcaatca tggttcgtcg tcttcttctt gttcagctgc
     1501 ttgttttagt ttgcggtatt ccccgagagt gacagtaatt tccgcgccaa gcaagacgat
     1561 acaccatgtc cagtagaccc aaacaaagag aatggggatc accgccagca caccgtaaat
     1621 gagctgatat gacgggaaca tggtgatata aagcgcgaaa cctttctttc ctgcttcgaa
     1681 caggagtgca gcaacaaacg cgccgacaat cgcgtcgcgg ttaggtacgc ggatggttgg
     1741 aacaatgctg tacagcaacc agaaggagat ccacgacaac agcagcggaa aaatacgcag
     1801 cacgttgtcg atgacagtat tgagatcgct cgcccagcgc agggagagca aataggaact
     1861 gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt aaaatcatcc agtacacggc
     1921 gaacgagtaa atttttggtc gagctcgttt actgcgccag atggtattca acgcgctatc
     1981 gatggagtac atcaacaata acgcggtgac gatcagcccg cacgccccaa cggcggtcat
     2041 cttgttggaa ttggcaacaa attgttcgat ataccgctga ataacatcgc cagtagcagg
     2101 cagaaagttg gcaaaaataa agtgacgcaa ctgaatgctg acgtcggaaa acatgggaaa
     2161 agcggcgaaa agcgcaaaaa caacggcaac cagcggcact aatgagagca acgacacata
     2221 ggcaaggtta cctgccaggg ttgtcatgtt gtcctcatca atgcgttgcc agagtagttt
     2281 tagccaggcc catagtggac gggtacggtg cctggctttg tcctgaatgg tttttagcat
     2341 aacaccttcg cgaaatagtc cgggatggtg gttttatctt tcaccagaat actggtaatg
     2401 cccagctgat tagctccttc tatattatcg gcgttatcgt cgaaaaagac cgtatcgctg
     2461 ggtgaaaaac cttccgcctg caaaacatgc tggtaaattc gagcttcagg tttgcgcatc
     2521 cccagatctt gcgacagata gatatggtca gcagcatcac gaatttccgg gtattcttcc
     2581 ggccagaagg tggtatgcag gcggttggta ttggaaagca ccaccacgcg atgcccctgc
     2641 tcacgcagtt tatgcatgat ggcgatcact tccgggcgca gcgcaacaaa cacagcctgc
     2701 cagccgtgag agaactgctc gtagcttagc ggtagagcca tctcatgaca cagcgcctct
     2761 gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat gaaacgcctc ccccatatga
     2821 aaactcttct taagcgtagc cagcggaata cgcgttaaat cgctccaggc tcccagcaca
     2881 cggttaaagt cgatatcgac aatcacatta cctaaatcaa agatatagag catttttgcc
     2941 tcctttcgcg ccacaagaaa ttaactgtag cgggaaagga tgagtttggc tatgtggcaa
     3001 taagggtaat cttgtgatgc tgattgcgaa aaagcagagt aatttcactg gctttttcgc
     3061 aatagatgaa tggttaagat tcaggtgtaa attgccaacg agtttcgata accagcggcc
     3121 tatctggtgt caccaattcc ggcgttagat ttattgcgtt aggtgcctga gtcagcgggt
     3181 taatacaggt ggcatcgggc tgtttatcaa ataccaccag actgtttgca gaagagatca
     3241 tggtcatcgc gagtttacca ggccacagta acttaacctt caccccgtca taaaaaccaa
     3301 aacagtcatc ccagggaccc gcttgaggag agatgcgatt tccagttggc agttcatcac
     3361 tacccgcttc ttcttgccag tccgcatcaa aaagcacctg caacgattct gtattttgcg
     3421 gagtcagctt tttcgcaaac cacggatgcc agcctgcgga ggccgggaat gtatcggcat
     3481 atgaatggac ttccagttgc aaaaccagcg catcgttctc cagcaaaaaa gtctgaatca
     3541 cttccccttg ccaaggccac ggtgaagcca ggggcatacg taaagtcagg gaatctgctg
     3601 ttttgtcgat gatttcccac gttgagtaac aagccatacc atgcaacgca tgtggtggtt
     3661 tattggcggg taaagaataa cattgcccac cagcattcag cgtcgcattc cccagtcgcc
     3721 ctgcccacgg caccattggg aaacagccat actgaaaagc tctacgctgc ggctgccatt
     3781 ggcgtaatac ttcagaacca aatgccttta acgaggttat cctggcacca tcatgcggat
     3841 aaatctgcag gtgaatattg cctttttcca gcgtcactaa ctctcctaca ggcccaggcc
     3901 atggcgcgtc aaacgcctgt tgtagcgcca ggtaatgctc gttttttcgt tcagttttca
     3961 tgagattgtt ccctgatgta gcggtaagaa gcggtttctt gttcatcggt cggctcaatc
     4021 agccagacgc aaacaaagga aagaactgag acagcaaata aaaacagagc gacataccac
     4081 ggttttcctc cccctagcgc cagcaaactt gaggcgataa gggggcttag tccgccaaga
     4141 atcgcggaaa attgataagc aaaagaaagg ccggtatatc gtactttggt accgaacatg
     4201 cgggtgaaaa gtgttggctg cacggcaaac atcatcgtcg gacctaagtt gtagccgagg
     4261 atcatactgc accagatgat gagcgtactt ttgctatcca gtaataagaa gaaaggaaac
     4321 gcgaacagga cacaaaatcc tgctcccgac aaatacagac ttttttgacc tatacgatct
     4381 gataaccatc caaccaatgg acaactgaaa ataccgatcg ccgaggcaat caacataccc
     4441 gtcaggggaa tatctcgcga taatgctaat tggctggaaa tatagacaat accaaaggcg
     4501 ttaataatat tagaggagac actttccgcc agcctcgcac ccagtgccaa taaaatattt
     4561 cgtggatgtt ttttgaataa ttcaatcaac ggaggaacgg acttttcttt attattcgtc
     4621 gttttttgct tttgaaaatc cagcgtttct tcggtatgca ggcggataaa cagcccaacg
     4681 atcaacatca ccgcagaaag ccaaaacgga atgcgccatc cccaggagag aaactgctcc
     4741 gaagtaagaa aatggttaca gagagcaaaa ataccggttg ccagcataat acccacagac
     4801 gcagccgttt gcgggagtga acctaaaaat ccgcgtcggg attcgggcgc tgattctatg
     4861 gtcatcaatg ccgctccacc gtactcgcca ccaagaccaa agccctgaat caaacgcagc
     4921 accattaata caagcggtgc ccaaatgcca atttcctgat acgtcggaat aaaaccaatc
     4981 aaaaatgtgg agcaaccgac aatagccagc gtccagataa gtgttatttt tcgcccgatt
     5041 ttatcgccaa aatgaccaaa gacgatgccc cctaaaggac gagtcagaaa accgacacca
     5101 aaggtcgcaa aggaagctat aagacctata aaaggatcgc tgtctggata aaacagcggt
     5161 gcaaatacca gtcctgctgc cgtgccaaaa ataaagaagt cataccattc cattaacgcg
     5221 cctgcgaaac tgcccataac gacacgtctc atggctttac cggaaagccc ttcacgcggt
     5281 gcgggtaact tttcagacat aattttcctc tcctggtagg cgagaaatct gggatacgaa
     5341 ccactaaacg tgggcagttt gaattaatgc gcatcggctt aagccgatgc gcattaaatt
     5401 atttcttcaa ttcagccaga acctctgcgt cctcagcatc aacatttgca cccagtggtt
     5461 cccaatgaat caatacgcag gtcaggaacc ccaggaatgg cacgattgcc aaccagtaaa
     5521 acgcttcagt tttcaacgca ccccacagca tcggccagaa aatgagtcct gcaattgccc
     5581 ctgtgcgcat aatagcgcgg gcaaatccga ccccagtagg gcgaatagcg ggaggataag
     5641 agagtgtggc aatggtcatg cccagcccac caggacccgc tgaatggaag aaaataattg
     5701 atcccaataa ccctaaagac agccagggat tagtcgttgc cagtgcgcct aacgacagca
     5761 atgctaaggt cacaacagca aatccataca tcgactgtcg gcgagtaccc agtcgttgga
     5821 gaataaacga gccgatcatc ccgcctgtca ccccacaaag ggcattcacg acggcagaac
     5881 cggtcaacgc gccagtaagc ccgccactta ttattcccgc caatgtaaga ggaagataaa
     5941 cacctacggc gttatattgc cacgcctgca tggttgccac gacacagcca agaatggtgc
     6001 gtttgcggta acgatcatta aataagatgc catatccacc actgtattta ttctctgctt
     6061 tttccgagaa tttcgcttct gttgtactcg atgccgcaac gcgagcctga acaccataac
     6121 gttttcccag aatgtcgcac gcttcctggt aacgcccgac gcgtgcggcc cacattgcgg
     6181 attcaccaat aaagaagtaa cgcaaaatca tgaagatgac tgcaaaaata gctccgatga
     6241 atattccgta acgccacaat tgttcttcag cgatacccga cagaagcagc ggcaaaagta
     6301 ccaaataaac aacggttgtc gaaacatacc aggccatttg ccagaggttg accgacgttc
     6361 cggttttctt cgacgttgaa ccacgaagct ctgccacggc attggttgcc aaagggaagt
     6421 cgatgcctaa accaaagccc atgatgacgc ggcacaccag cacccaccag atactgggag
     6481 caatagctac cgctgcagca ccgatggtgc acagccccat gccaataata aatgcccgct
     6541 tttgcccaaa gcggtttatt actcggttcg ctaataaacc gccaattaac gcggcgatta
     6601 atactgatgc attgacggtt gcagcaagac ccggcgaaat gccgaattga gcggcaatgt
     6661 atttattacc aaaccccacc atcgccgcct ggtaggcatc aatcaatatg ccgcccaaag
     6721 caataaaaat aacgccgata ctggtgttga ttgcagagtt cttattaaca atatcaatta
     6781 cgtcctgtgg ccgactaatt gaataagttg caggactggt gatttgactc atcgcagtgt
     6841 cctttttaat tgttgtaggg tggcgctgac gtgacgcagc gccagacaga gatttagaaa
     6901 atcattccgg cgcaaattgc agcatgacct ttatggagtc attttgctgc gcgcgttcga
     6961 atgccgccgc tgcatcctca atggcaaata aatcggtaac cagttcactg gtatcaaacc
     7021 gcccatccgc cagccattcg ataacagcat cgaaatcttc agggatgtac atgccagagt
     7081 tgagcaagtc acgctcgaaa cgttgcatca tcggtaatgg tatttcacgc ggcccacttg
     7141 gtacgcccat gcacacgacg gtgcctccgg catatacacg ggtacatgca tcgttaagcg
     7201 tggcctgcgc tgcaacgaca tcaatcgccc ccgtaaatcg cacatctgcg gccagctcac
     7261 ctggtgccca aacttctgct gcgcccagct ttaacgccag cgcgcgtttg gcagcgtcag
     7321 gttcaatcac ggtaatctta cctgccccca taatgcgcag tgcctgcacg atggataagc
     7381 ctatcgttcc ggcaccaatc accagtacgc tttccagaga agctttcggc attcggttaa
     7441 cgcagtgacg ggcacaagcc gccggttcgg caaaggccaa cacttttagc ggtaaagagg
     7501 ctggtgcgac atggcaatta cgcgcaggaa caatgtgttg tgagcgagca aagcccggtg
     7561 cgcgaaaacc agcaacctgc ggtggttcac aaagattaaa acgtcctgct ttgcaggctc
     7621 ggcattccat gcaagccatg atgggatcga ccacaacatg atcgcccggc tgtacatttt
     7681 taacgtcgct gccaacttcc gtaacgcgcg ctgcaatttc atgaccgggg acgacaggtg
     7741 gtttggcaaa cggatgaccg cctttcagaa catgcagatc cgaaccgcat atcccataga
     7801 acacaggcgc aatccgcact tcgtgcggtt taagttcctg gtgtggtact tcaacatatt
     7861 gagtggtgac tttcccaata tctgaaaata aaacctgggt aattttatcc attgttattc
     7921 tccagaatcg atatccatag gggattagcg ttgcgcgata ccttcatcga caatttctgc
     7981 caaccgttgt aaacgaggaa cagagacatc gcgcagcata atttgtggat cttgtgcacc
     8041 aacaaccgac gcccaaaccg ctcgtccagc caggaagcct gacgcaccac ctttcatggc
     8101 aatacttact gcacggccaa aaatatcggc atcaacgcca gaagaaagaa ttacccacgg
     8161 catcttcatc tgatcgttaa gttgctgaca ggcggcaagt aaggtttttt catcgccttt
     8221 gccccctaat ggcatctcgg ctttgtaaag atcagcctct gttccaccaa gctctgcggc
     8281 ggcggcaaca atggcgcttt cacgatcaaa gtcccaaccg cgacgaggtg gacgcaccac
     8341 aggttcaata atgctaacca gcccggcact acggcagcga cgaacaaatt tatcaaccat
     8401 tgccagacgt tcctctgcgg gttcatcttc acgccagagt acaagtaatt tcattgcctt
     8461 agcccccatt tcacgggctt tatgcgggtc gacggacata tctatttcaa cgctatcaac
     8521 cgggatgcca ttacctggga taaaaagatc ggcagcgaca atcaggccgc aagaattagc
     8581 aacagcgccc tgctcgacta tttgctcaag acaaaattgt ttatccgcca gaacggcaga
     8641 agcataagga gaaaggattc gggtcgcggc aactttaaaa tcggtgagta cactgtcggc
     8701 gatgggtttt ggctgccctg ctgccgcaaa catcaagcgc atggcctcac gctgatcgac
     8761 agctaacatg gcaaatccac cacctgggcg ggagatatct ttcagcgtaa atggggtgaa
     8821 gtgggatgac atagaaactc ctgttaatag ataaaattaa agaccagcct gctggcgaac
     8881 ctgatttagc agggcactcc agtcctgccg ccctttgccg tcttgacttg ctgcagcgta
     8941 ataactttct gctgcaatcc ccatattgag cggtgccccc gtctcccgag cgacgtcaac
     9001 tgcaatacgc agatctttca acgccagatc caccatgaag gcgggtgaaa gatcgccttt
     9061 cagcactttg ccaggccagg tggtcgtaaa atgtcctttc cctgctgccg taccactcat
     9121 caccttaatt gcgacatcaa gatttaatcc cagtgactca cataacacag cggcctcgga
     9181 ggacagcgca ttaagcgcaa tgctcatata gttgttaatt agcttcacgc ggatccccat
     9241 tccagggccg ccagcctcga ccagctcatt acccatgcac atgagaatcg ggcgtgcacg
     9301 tgcaatctgc tcatcagtgc cacccgctaa aatcagcaat gtgccatcaa tcgcattcac
     9361 ggacgttctg cccacggggg cgtccatcat actgatgcct ttttcttgta actcccgtat
     9421 taatgcatcg gtctgtaagg gatgaatagt cgacatatca atcaataacg cgtcagatga
     9481 aacagcctca cagacgcctt tttcccccaa cagaacctgt cgaacaatgt caccattggg
     9541 aagcatagtg atgataaatt cagcgcctgc tgctgcttcc gctggggttt gtgccgcagt
     9601 ggccccctgc gtaactagcg catcgacggc ctgtgcattg acatcaaata cctgcaatga
     9661 atgcccttta tgcaatagat tgttagccat tgggctaccc atctgcccca gcccgagaaa
     9721 tgcaatggtt gccatgaata ctctcctgtc tgacattgac aaaataatga caattgaaaa
     9781 ttcgttttcg tctgtttttg agcatatttg taatttatgt tcaaaaacac gacatacatc
     9841 actaatttga tcatttgtgt catttaaatt gaccaaagac gacaaaatta aacaggacaa
     9901 gacaaatatg accagaattg catgcgtggg tatcacagtt caggacagga tttattcttt
     9961 gcccactctg ccggagggcg gcgggaaata tcaggcaaat cattatcttg agattggtgg
    10021 cggcccggca gcaacagccg ctgttgcaat cgcaaggctt ggcgtggaag ttgatttcat
    10081 cggtcgcgtc ggcgatgaca gttgtggaaa taccttactt gcagaacttg agggttgggg
    10141 cgtcaataca gcattttgcc gccgatatcc taacgcccgt tcgtcgcaat cagcgatcct
    10201 cgttgatcag catggcgaac ggattatcgt taattatcca agtcctgatc tcggtactga
    10261 tgccgaatgg ttagaagcga tcgatttctc tcgttatgat ctgattctgg ccgatgttcg
    10321 ctggcatagc ggtactgaaa aagcattctc gctagcgcgc cttgcgggtg tgacaacgct
    10381 tttagacgcc gatatgacac cgcaggatat ttctccactt gtcgcgcttg ccgatcacgc
    10441 cgtgttctca acgccagggc tgaaacgcat gactggcctg cagtcacctg aagaaggttt
    10501 gttccaggca acgacacaaa cagcaggtaa agtgtatgtt acgctgggtt ctgaaggatc
    10561 gttgtggatt gaagatggtc atttgtgtca acaggaggca ttttctgtca atgtggttga
    10621 taccactgga gcaggcgatg tttttcacgg cgcactcgcg gtagcgttgg cagaaaaaat
    10681 gccaacaaaa gaggctattc gttttgccag tgcggttgcc gcgatgaaat gtacccagcc
    10741 tggcggtcgg gccggcattc ctaatcgtga gcaaaccgaa tcatttttgt cactttatgc
    10801 gtaaaatggt aaacatctac cgaggagggg caagggagta ttcatgagta tcatcgaagt
    10861 gacaggtaat ccacgccatg accaactcgt tcatctaatc gcagaacgcg gttatatgaa
    10921 tattgaagaa ctggcacaac tgctggatgt ttcaacgcaa accgtgcgcc gggatatccg
    10981 aaaactcagt gagcagggct tgatcacccg tcatcatgga ggggccgggc gcgtctccag
    11041 cgtcatgaat accgcttttg agcaacggga actttcgctc accgccgaga aacgggcgat
    11101 cgctgaggca gtcgccgatt accttcccga acgctgtacc gtctttataa ccatcggtac
    11161 aaccgtagaa gccgttgcca gggcattact caaccggcgt gatttacgca ttatcactaa
    11221 cagcctacgt gtggcacaga ttctttataa gaatcaggat attgaagtga tggtgccggg
    11281 aggcacttta cgcgctcata acggcgggat tatcggccca ggagccgtgg attttattga
    11341 aggcttccgc gcagattatt taatcaccag tataggtgcc attgaacatg acggcaccct
    11401 actggaattt gatgtaaatg aagcgttagt cgcgagaacg atgattaaac atgcgcggaa
    11461 tacattgtta gtggccgatc atacgaagtt tgccgcgtct gctgccgttt caattggcaa
    11521 tgcacggaat gtcagggctt tttttactga tgccccgcct cccaattctt tctgccagtt
    11581 gttaagtgaa gagaatgttg aactggtggt tgccgagcag gaagtttcct gaaattgaaa
    11641 cttcgaaaaa tccgctgggg attcagcgga ttttattttg ccttatccgg cctacggttg
    11701 atacggtttt cgttggccgg attaagcaca tccggccatt aattaagctt cagccgccag
    11761 catctctttc ttacgttggc taatacgctt aaccagcatt acggaaacca ccgcaccaca
    11821 gaatgcgaag caggccataa tgccaaacac gattttgtag ccaataatcc ccggatttaa
    11881 atccagaatg tagccataca gactgaagca gaacatcgcc ggggcgtaac caataaagct
    11941 acccaacgcc atcgccgcgc ctgttttatt ttcagcaatt tttgcttcgc cgataggtgc
    12001 aaaaaatacg gctcgctgtg taaatactat cgcgccaaag cccagcgtac atgccatccc
    12061 taaatagacc ggcatacttt cgtgcggcag cataatcaac agtacgagcg cagcggtact
    12121 gatgataaag gtgtagcata gatatttact cggcgatttc aggatcttat ctgaaatcat
    12181 gccaccaatc ggtccaccaa tcattttcag acagtattgg ttaatgatgc cgtaagcccc
    12241 caccagcgca acgggcaata gatagatgtt tttcaggaat ggaatgaaga atgtcaggcc
    12301 acagtaaacc gcgtaaacga agaagacgtt gaaagcgata agccagatgg ttttgtctct
    12361 cagcaccgag gtcatactgg tgttttgcga tgctccgtct tcttttttca cctcaacgga
    12421 cggtgcctct tctttgtcat tcaggacaaa gaaaataata atgcctacgg caatcacaat
    12481 aagggaatag aaccagatgc ctgctttaaa acccaataag ccactgccaa accaggtaaa
    12541 tactgccaac gcagaaaatg ccaccacggt atcgacaatg ccacgccctg tttcgaagaa
    12601 gccaaacaac cgaccttgtt gttcgctatt gcccaatcga cttaccgact tgagcaagac
    12661 cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc cagacaaaga ggatgcccca
    12721 ataccccggc attgtcgtca aataaacacc cagtaatcct gtcgcaatga gtgagaatga
    12781 catggtgtat ctgcgcggta gtttatcggc aaaataaata gacagaaaaa agcccactgt
    12841 ggtaacaaat gagtttaccg acatagcatt accaatttga ccattggtca aatggaaata
    12901 ttcctgcatc gggatataaa acgcatcttt cagcgacggt aatttataaa ttgtaccgcc
    12961 acacagtgtt aatagactaa ataacgccca tttctttttc gtgagcataa catcccctaa
    13021 gcgattttta aaatcactgt tttaaaggca ataagatacc gaccattcat atggaatggt
    13081 tttgttaact gcgtatgagt taaaacttat tagtggttca gaagatggtt tgcctcttgt
    13141 ttgatttgtt gtagtacgtt acgcacatga tttatctgat taattgcatc ttctttttct
    13201 ttacgtaacc gcgcatccag taaatgattt tctggcaatg gtgtttcact catctggcga
    13261 taagggatcc acggattatc atcttcgtct tcaaagcgga acgccggcgc ataataatca
    13321 acctcttctt ccaggccata tgccgtcacc tgcggctcat catcacccag gcagataagg
    13381 tgcgttaata acgctttgaa cggcatatca ccctgacctg aaatacaggc tttatgaccc
    13441 aggccaccct gttctttaac gatcaaggca tctttgatat ggacctgggt aatatgcggt
    13501 gccatcgttt ttaaggcgtc tatgggatgc tcatttgcat tgatcatgtt cgcaaaatca
    13561 aacagtaagg aaagagactc catctcactt tctttgacca gcgacaccaa ctcatgactt
    13621 tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc tgtcctgata cgtttcccgt
    13681 acataggcaa tgtcgttagc gataatcgat aatacgtcgc gcaaattacc ttcataacgt
    13741 ggataaaaac gtacggacga tgccccagtt ttcaacgcga tggcgacggc ttcgtcaata
    13801 gatgccttat ctgaggcgct ggtttcaata tgaatatcaa ggttcagacg gcgggcttta
    13861 tctccaaagg cagagagttc tttatcgtcc atattaccaa gagaaaaacg ctcgccatcc
    13921 agaacatgga ttttgacgcc acgtagatta ttttcacttg cgatatcaag taaatcagca
    13981 ggcaacactc tttccagtcg catatttaaa tgaaaggcat aagcatgtaa ataaagagga
    14041 agagtgtcga cacgttgtag aatctttctt gcgttattta ttgtcaccat aaaacaccta
    14101 cagattaatg atgaggtcta cggcctcaaa tcgccaatat tcaatatcta tctggacaat
    14161 attgccttgc tggtcggcgc gatgcttttc aattaagatc gctggcatac ccgcagaacc
    14221 gccaagatac ttgctgatat cgcccggcat tctgacgggt ttgaaaatca atttttttac
    14281 taccgtctct ttttggtagc acttttccca gactgcagaa aatgagtggt tttcaagttg
    14341 ttcaataaag cccggagcaa cttcaggatt aatatatgtt tcgtgataga aaactttatg
    14401 tccttccagc gccccccagc cggtgatgcg atagagttca gttgatggcg tcactgcaat
    14461 cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt ttctcggtaa acccccagga
    14521 aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc gccgacagct ccggactgta
    14581 attaaatcgc ggctgggtca cgaaccaccc tttacgatcc ttacggaaga ttttggattc
    14641 tgcctcgagg ttcaacaacg cctggcgcaa cgtcatacgt ttaatgccca gcagctctcc
    14701 cagctctctt tctgaaggga gttttccacc cggcgtggtg atgccatctt tcaaccaccg
    14761 atccagtttc tcttttgcat tttctacggt ggattgattc tccgccattg tttaatcctt
    14821 ttggtttaaa ccagatgaag catcttacgc ttacgccaaa aaagaaagca attaacggcg
    14881 tatgactttg cagataattg tgacaaagct ctctattgac gtaaccacag acttataagg
    14941 gagatactcg cattggtgtg cgctcacgac aggcaaaaaa aaacctgcca tcgctggcag
    15001 gttttttatg actaaaaaac gaaattaatc gtctttcggt gcgcggttgg cgcgacgacg
    15061 atcgttttcc gtcaggtgac gtttacgaat acggatagag gtcggagtca cttctaccag
    15121 ttcgtcatca tcgatgaact ccagagcttg ttccagagtc atgcggatag gcggaaccag
    15181 aacaacggct tcgtcagtac cggaagcacg catgttggtc agtttcttac cggtcaggca
    15241 gtttacagtc aggtcgttag agcggctatg aataccgata atctgacctt cgtaaacttc
    15301 tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga ccgaacagcg cgaacgcgac
    15361 cgctttaccc tgaccgttag agatcagtac accgttctga cgctgaccca cttcgcccgg
    15421 acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga cccgtaccgg aagtcatggt
    15481 catgaactca gaacggaagc caatcagacc acggcttgga atcacgtagt cgagacgtac
    15541 gcggccttta ccgtctggat tcatgttttt caggtcgcct ttacgttcgc ccagcgcctg
    15601 cattacagaa ccctgatgct gttcttcaac gtccagagtc acgttttcat acggctcttg
    15661 tttacgaccg tcgatttcac ggaagataac tttcggacgg gataccgcca gttcgaaacc
    15721 ttcacgacgc atgttttcga tcagaacaga caggtgtagt tcgccacgac cagaaacgcg
    15781 gaacgcatca gcgtcttcgg tttcttctac gcgcagcgca acgttgtgta ccagttcttt
    15841 gttcagacga tccaggatct gacgagacgt tacgaactta ccttctttac cgcagaacgg
    15901 cgaggtgtta acgcagaaga acatagaaac ggtcggctca tcaacggaga gtgccggcag
    15961 cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg ttcagttcgc ccaggcccgt
    16021 gatcgccacg atatcgccag cttccgccag atcggtttcg atacgttcca gaccgaggtg
    16081 gcccagcact ttaccgactt tcgcgttacg ggttttgcct tcgctatcga tgatagtgac
    16141 ctgctggttc ggcttcactt taccgcgctt gatgcggcca atgccgataa cgccaacata
    16201 gctgttgtaa tcgagctgag aaatctgcat ctggaacgga ccgtcaaggt caacgtccgg
    16261 cgcaggaacg tgatcaacaa tcgcctgata cagcggggtc atgtcttccg ccatatcttc
    16321 gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa acgatcggga agtccagctg
    16381 ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc tgatccacaa cccaatcagg
    16441 acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc ggcttcaggc cgtaagcaaa
    16501 cgcttttttg gttacgaagc gcgtttgcgg catcgggccg tcaaatgcgt caaccaccag
    16561 cagcactgag tctaccatgg acattacacg ttcaacttca ccaccgaagt cggcgtgccc
    16621 cggggtatca acgatgttga tacggtaatc attccatttg atagcggtgt ttttcgcgag
    16681 gatggtaatc ccacgctctt tctccaaatc gttggagtcc atcacgcgct cttgggtttc
    16741 ggcacgagag tcgaacgtac cggattgttg gagcagcttg tctaccaggg tggttttacc
    16801 atggtctacg tgcgcgatga tggcgatatt acgcaatttt tcgatcacaa ctttgcctca
    16861 ggcattagaa atagcgcgtt attgtacacg gattaatcgc actacaaaac aggatcacaa
    16921 acatcctccg caaacaagta ttgcagagtc cctttgtgat cgctttcacg gagcataaaa
    16981 agggttatcc aaaggtcatt gcaccaatat ggtgcttaat gtttccattg aagcactata
    17041 ttggtgcaac attcacatca tggtgcagcc cttttgcacg atgttaagca tgataacgcc
    17101 ttttaggggc aatttaaaag ttggcacagt tttcgcttta tcttttttac ggcgacacgg
    17161 ccaaaataat tgcagatttc gttaccacga cgacaatgac caatccagga gagttaaagt
    17221 atgtccgctg aacacgtact gacgatgctg aacgagcacg aagtgaagtt tgttgatttg
    17281 cgcttcaccg ataccaaagg taaagaacag cacgtcacta tccctgctca tcaggtgaat
    17341 gccgaattct tcgaagaagg caaaatgttt gacggctcct cgattggcgg ctggaaaggc
    17401 attaacgagt ccgacatggt gctgatgcca gacgcatcca ccgcagtgat tgacccgttc
    17461 ttcgccgact ctaccctgat tatccgttgc gacatccttg aacctggcac cctgcaaggc
    17521 tatgaccgtg acccgcgctc catcgcgaag cgcgctgaag attacctgcg ttctactggt
    17581 attgccgaca ccgtactgtt cgggccagaa ccagaattct tcctgttcga tgacatccgt
    17641 ttcggatcat ctatctctgg ttcccacgtt gccatcgacg atatcgaagg cgcgtggaac
    17701 tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc cggcagttaa aggcggttac
    17761 ttcccggttc cgccggtaga ctcggctcag gatattcgtt ctgaaatgtg tctggtgatg
    17821 gaacagatgg gtctggtggt tgaagcccat caccacgaag tagcaactgc tggtcagaac
    17881 gaagtggcta cccgcttcaa taccatgacc aaaaaagctg acgaaattca gatctacaaa
    17941 tatgttgtgc acaacgtagc gcaccgcttc ggtaaaaccg cgacctttat gccaaaaccg
    18001 atgttcggtg ataacggctc cggtatgcac tgccacatgt ctctgtctaa aaacggcgtt
    18061 aacctgttcg cgggcgacaa atacgctggt ctgtctgagc aggcgctgta ctacattggc
    18121 ggcgtaatca aacacgcgaa agcgattaac gccctggcaa acccgaccac caactcttat
    18181 aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg cttactctgc acgtaaccgt
    18241 tctgcttcta tccgtattcc agtggtttct tctccgaaag cacgtcgtat cgaagtacgt
    18301 ttcccggacc cggcggctaa cccgtacctg tgctttgctg ccctgctgat ggccggtctt
    18361 gatggtatca agaacaagat ccatccgggc gaagctatgg acaaaaacct gtatgacctg
    18421 ccgccggaag aggcgaaaga gatcccacag gttgcaggct ctctggaaga agcactgaac
    18481 gaactggatc tggaccgtga gttcctgaaa gccggtggcg tgttcactga tgaagcgatt
    18541 gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg tgcgtatgac tccgcacccg
    18601 gtagagtttg agctgtacta cagcgtctaa tagttgaagt tgtaataccc ggcgcaacaa
    18661 cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg
    18721 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
    18781 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
    18841 tcaactcgct gattaacagt attttgttaa tcgatgacaa cctggcgatc cattacgcca
    18901 accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acaccgttac
    18961 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg
    19021 ggcaaggttt taccgataac gaagtgacgc tggtgatcga cggtcgctcg catatccttt
    19081 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggcg ccgatggata
    19141 accagcgccg cttaagccag gaacagctac aacatgctca acaagttgct gcccgtgatt
    19201 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
    19261 agctactcag caaagcatta cctgacccgt cactgctgga atataccaaa gtgattatcg
    19321 aacaggcgga ccggctgcga aatctggtcg accgtctgtt ggggccgcaa ctgcccggta
    19381 cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt ggtgacgctg gtgtcgatgg
    19441 aactgccgga caacgtgcgg ttgattcgtg attacgaccc cagcctgcca gaactggcgc
    19501 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
    19561 tggggccgga gggtggagaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
    19621 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccaggcattc
    19681 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
    19741 ggcttggctt atccatcgct cgtaatttga ttgatcagca ttcaggcaaa attgaattta
    19801 ccagttggcc agggcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
    19861 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
    19921 tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaggt
    19981 actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
    20041 aatggacggg ctggcactgc tcaagcagat taaacagcgc catccgatgc ttccggtcat
    20101 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
    20161 tgattatctg cccaaaccgt ttgatatcga cgaagccgtg gcgctggttg agcgcgccat
    20221 cagtcattac caggaacagc agcagccgcg taatgttcag cttaacggcc caacgaccga
    20281 tatcatcggc gaagcgccag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
    20341 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
    20401 tgccctgcat cgccacagtc cgcgagccaa agcgccgttt atcgcgctga atatggcagc
    20461 tatcccaaaa gatttgatcg aatcagaact gtttggtcac gagaaaggcg cgtttactgg
    20521 cgcgaatacc attcgtcagg ggcgttttga acaggctgat ggcgggacat tattcctcga
    20581 tgaaattggc gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagatgg
    20641 tcagttttac cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
    20701 cactcaccag aatctcgaac agcgggtgca ggaaggtaag ttccgtgagg atctgttcca
    20761 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc
    20821 ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt
    20881 gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtgcgcca
    20941 gctggaaaac acctgccgct ggctaacagt gatggccgcc gggcaggaag tgttgattca
    21001 ggatttgccc ggtgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
    21061 ggacagttgg gcgacacttt tagcgcagtg ggcagacaga gcgctgcgtt ccggtcatca
    21121 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
    21181 acatacgcag gggcataaac aggaagcggc gcggctactt ggctggggcc gtaacaccct
    21241 gacgcgtaag ttaaaagagc tggggatgga gtgattcacg gcttgtgtgt aaagattgat
    21301 tattgagcgc aaattgctgg tattttgcgc tttactgttc cgataagttc agtatgatct
    21361 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
    21421 cgcagttgac agccacacac cgcaaactgc tgttgctgcc gtgctgtgtg ccgcgatgat
    21481 tgggctgttt agttgagttt cagattgctg atgaagtgct ctttgttcat gccggatgcg
    21541 gcgtgaacgc cttatccggc ctacaaaagc aatattttca acaggttttg taggcctgat
    21601 aagcgtagcg catcaggcaa tcttgttctc agcacattga tggcgctttc gttttctact
    21661 ttgtaaacga agcgccattc actatttaaa tcacccgaga gaactgctgc atccgcgctt
    21721 tctggcgcag ataggtatca aagcacatgc aaatgttgcg gatcagcaag cgacctttcg
    21781 ccgtcacctg tattcccttc tcatccacat ccaccagccc atcttttgct aacggggcga
    21841 gcagcttgag atcttccgca aagtaatcag cgaagtgcaa atcccactgt ttctcaatag
    21901 gggcgtaatc cagacggaag ttgcagatga gcgacttaat cacatcgcgg cgaatacagt
    21961 catcacgcgt tagcgcaata ccacgccaca gcgcattgcc ttgttcatcc acttgctgat
    22021 agtactgctt caactctttc tggttctgcg cgtagcagtc gccaatcata ctgatggcgg
    22081 aaacgcccat ccccagcaga tcggtatcgc cctgagtggt gtagccctga aagttacgat
    22141 gcagcacgcc ttcacgctgg gccaacgcca gctcgtcatc aggacgggca aagtgatcca
    22201 taccgataaa ctgatagccc gattgcgtca ggaaggcgat ggtttcctgc aggatatcga
    22261 gtttttgctg cggactcggc aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg
    22321 tcggcagatg cgcatagtta aagacgctca gacggtcagg gtttagctcc gccacgcgtt
    22381 tcagggtaaa ggcgaaactt tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt
    22441 tggtggaggt aaagccaatc tcacgcgcat ggttaagcag cgcaaagata aactcttcat
    22501 cctgctcgcg gttaaccaga cgttgcactt ctttgttgaa gtcctgcacg cccatactca
    22561 ggcgattaaa gccctcggcg cgtaaatgat cgagtacatc cagttcgatt tcccgcggat
    22621 cgacttcgat cgaaatctcc gcatcggcat tgaactggaa gttttcgcgc agcagcttca
    22681 tcaggcggct gatttgcgct ttattaagat acgtcggcgt tccgccgccc cagtgcagct
    22741 ggctgacgtg acgcccggcg aacagcggtg cacgatggac gatttcttgc tccagcgcgt
    22801 ccagatactg atcggccttg tgctgctggc gagtaacaat cttattgcaa ccgcagaagt
    22861 aacaaagctt atggcagaac gggatatgta cgtagagaga taatggacgc tcaggatagc
    22921 gcgccacggc ttgtaaaaac gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg
    22981 ggtacgaggt gtatcgtggc ccggaatagt tatatttctg gatcagggcc agatcccagt
    23041 cgatttgctg tacagacatg ctcactcctt ccgatggcgt ctctggcggc tacggcgtac
    23101 cggcggatgc ccgtttcggg tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa
    23161 ccgctgtagt ttaacgaata accacaccag ataacatata accggaaggg ttatcagcag
    23221 aacgggaagg cccacggcgg caatccgtta gttgccccgc agcagacgca tcatgtcttc
    23281 ttgcttctcg tcttcttcct cttcttcgtc atcgtcataa gagaggccga gtttctgcat
    23341 caactcatca atacgatcca gtttagcatc cacccaggat tgctcttcgg cactcagggt
    23401 ttcgcctgct tccagacgtt ccagcagcgc atccagacgc tcatccgttt ccagtaattc
    23461 caactccgcc tgcggtgaaa gcataggttt ctcactcttc ggtttgtgct gtttggtgac
    23521 tttttcagtc acacccaatg gaataggggt tttactgcca atacgtggat ctttcggtgc
    23581 gttctggcct ttgctgcctg acgtgttgtt tccgcccgct gcgcggctgc ccggcgcatg
    23641 accacgacgt tttttctgac gcttgcggtc acgagcttcc tgattcagct cttcgcgcgt
    23701 tttgcgacgc gcttttgcat gacctttgct gcgtgatggt gaagatggtt tcataatggt
    23761 tcgtctttta gccgttttat tcagtataga tttgcggcgg aatctaacag aaagcaagca
    23821 aagaaaaaag gcgacagatt actctgtcgc cttttttcct gactcataac ccttaacggg
    23881 tcttacaatc cttgcaggcc gccaacacac cctgtttttc cattagccgg aacaatccgt
    23941 gttgattcca tttccgttta attacgtctc cggacgtttg tcttcctgac aatcctctgt
    24001 cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt gttcctgtta gcgtcctcgc
    24061 tctatgggtg ctactttacg cctttgcttt aaacaaacaa tcaaccgcca tcagtatttg
    24121 cacattttca gagttttata taaaagcaat taactgattt atatagctta tttgacatgc
    24181 atcgcagaat tttctctgct atttcgcata aagactatcg gcaatgtctc acaaagcgta
    24241 tggcttttac ttacaacgct cacgccgaaa aatccgcctt ttattatggt tcaggtataa
    24301 tcgccgcaaa gctttgactg acggagacga ccgctttgac taatttgaat tatcaacaga
    24361 cgcattttgt gatgagtgcg cctgatattc gccacctacc ttccgatacc ggaattgaag
    24421 tggcttttgc aggccgttcc aacgcaggta aatccagcgc gctgaacacg ctgactaacc
    24481 agaaaagcct ggctcgtacc tcaaaaaccc caggacgcac ccagcttatc aacctgtttg
    24541 aagtggctga cggcaagcgt ctggttgact tgcctgggta cggttatgcg gaagtcccgg
    24601 aagagatgaa gcgcaaatgg cagcgcgcgc tcggcgagta cctcgaaaaa cgtcagagcc
    24661 tacaaggtct ggtagtgcta atggatattc gccatccgct gaaagatttg gatcagcaga
    24721 tgattgagtg ggcggtagac agcaatatcg ccgtgttggt actgctgacc aaagcggaca
    24781 aactggcaag tggcgcacgt aaagcgcaat tgaatatggt gcgtgaagcg gtgctggcgt
    24841 ttaacggtga tgtgcaggtt gaaacgtttt cttcgctgaa gaaacaaggc gtggacaagc
    24901 tgcggcagaa actggatacc tggtttagcg agatgcagcc tgtagaagaa acgcaggacg
    24961 gcgaataatt ttcttgcctt aatgcttgtg ccggatgtgg cgtatccggc ccgtaaattc
    25021 aatagattac aattctaccg ttggcctgat aaacatggcg catcaggcat tgcggatttt
    25081 ttctttcgcc caataaaaaa cgccccagtc attactgact ggggcggcta aaatattcag
    25141 ccaaatccga ttacgtgaag taaaagatct gaaagataga acatcttacc tctgtaccct
    25201 acgcgactaa ctttactctt ttttgttcag ttcagaaagc actttttgta attttttttc
    25261 agttgttgca taggaatttc taatagccat cacaaaacgt gacagcttat gttgcttact
    25321 tacgaaaaaa gggcatgttc aggcgaatct tagtgcgcct gatcccagtt ttcgccactc
    25381 cccacttcca ccagcaacgg cacatccaga cgggtacagt tttccatcag ttgatgaatc
    25441 tgcttcgcga cggcatcgac atcatcttta tggacttcaa ataccagttc atcgtgtacc
    25501 tgcatgatca tacgtacacg cggttgctca gcctgtaacc acgcatcaac ggcaatcatc
    25561 gcccgtttga taatgtcggc ggcggttccc tgcattggcg cgttaatggc tgcacgttca
    25621 gccgctgcac gacgagcacc attgctggat ttgatatccg gcagatacag acggcgtccg
    25681 tccagcgttt caacgtagcc ctgctctttc gcctgagcac gggtgcgttc catatactcc
    25741 agcacgccag ggtagcgttc gaagtaaagg tccatgtact tctgcgcttc tttacgtgga
    25801 atgttcaatt gccgcgccag accgaaagca ctcatgccat aaatcagacc aaagttgatc
    25861 gctttcgcgc tacggcgttg ctcgctggtg acggtttcca gtggcaaacc aaacacttct
    25921 gccgccgttg cccggtggat atcttttcct tccgcgaatg cggtcagcaa gcctttgtca
    25981 cgcgagagat gcgccataat gcgcagttca atctgcgagt agtccgctga gacaatcaca
    26041 taatcctctg gcgcaataaa cgcctggcgg atacggcgac cttcttcgtt acgtaccgga
    26101 atgttttgca ggttaggatc ggttgacgat aaacgtcccg ttgcagttac tgcctggtga
    26161 taagaggtat gcacacgccc ggttttcggg ttgatcatca gcggcagctt gtcggtgtag
    26221 gtcgatttca gcttcgccag accacgatac tccagaatca cttttggcaa cggatagtcc
    26281 agcgccagtt cttccagtac ctcttccgac gttgacggcg cgccacccgg cgttttcttc
    26341 agcggtttaa tgccctgttt ttcaaacaga atggtttgta actgcttggt ggaagaaagg
    26401 ttaaattcct cacctgcaat ttcatgcgct ttcttttcca gctcagccag acgcaaggta
    26461 agctcttcag aatgattgtg cagcactttc ggatcgatct tcacaccgtt acgttcaatg
    26521 cgtgaaagca ccggcaccag cggcatttcg atattctcga agacgttcaa cggccctttg
    26581 tgtttttgca gatccggcca cattttcaga tgcaactgca aggtgacatc tgcatcttcg
    26641 gcggcgtaac gtccggcctc ttcgagggca atctggttaa aggtcagttg atttttgcct
    26701 ttacccgcaa tctcttcaaa agtgatggtt ttgtgcttca accaacgttc tgcgaggctg
    26761 tccatatcgt gacggccagc aacgctattg agaatgtagg actccagcat ggtatcaaac
    26821 gcaatcccac gcagctcaat gccgtaattc gccagaatac cgcgatcgta tttcaggttt
    26881 tgcccgacct tcagcgcctt ttcatcttcc agcagcggtt ttagcaactc gagtgcacgc
    26941 tcgcgagaga tttgatcggg cgcatcaaga taatcatgag caaccggaat atatgccgct
    27001 acgcctggct cgatagcaaa agaaagcccg accaggttag cagagatgtt atcaaggctg
    27061 tcggtttcgg tatcaaatgc aaataccggc gctttttcca gcttcgcaat ccacgctttc
    27121 agtgtttctt catcaaggat ggtgacgtag ttgtcataag aaatcaccgt tgccgtcact
    27181 tctggtgctt cgtctgcaac actggtttcc tgtggcttcg cggctggttt tgcccctttg
    27241 gcctgtaacc atttgcccgc ttcgacatca gcagtccagc gtttgaactc atactttttg
    27301 aacagcccca acaactcttc cgctgccggt tgctgcactt ccagttgttc acaggtcagc
    27361 tccagttcaa cgtcggtttt aatcgtcgcc agctggtatg agagataagc aacttctttg
    27421 ttttgctcga gcttcgctgc cattgttttc gcgccacgga agctcaaccc agcaattttt
    27481 tctggctcgg catacagcgt atccagcccg ccaagacctt gcagcaatgc ctgcgcggtt
    27541 ttttcaccga cgcccggtac gccaggaatg ttatcagagg agtcacccat cagcgccagg
    27601 aaatcgatga tcagttctgg cggcacgccg tacttattca ccacctcttc cggtccgagg
    27661 atggtattcg tcatggtatt gataagcgta atatttggcg tcaccagctg cgccatatct
    27721 ttatcgccag tgctgatcag caccggacgc ccggcttttt cggcttcgcg cgccagagta
    27781 ccgataacgt cgtccgcttc tacgccagaa accgccagca gcggcagtcc catcgcttta
    27841 accatcgcgt gcagaggttc gatttgtgct cgcagatcgt ccggcattgg cgggcgatgt
    27901 gatttgtaat gttcaaacag ttcatcacga aaggtttttc ccttggcgtc aaagaccacc
    27961 gctgcatgcg tcggtttata ttgcatgatc agactgcgca gcatgttgag gacaccatac
    28021 atcgcaccgg tcggctcgcc tgcgctgtta gtcagcggag gaaacgcgtg atatgcgcga
    28081 taaagataag atgaaccatc tacaaggata agtggatttt gggggatctg aaccataatg
    28141 tccgtgcctg tttatcagat tatgggtaaa ggatgccaca gacaggataa aaacatgagt
    28201 ttttcaccgc ttttcatgaa aatcttttgt agatcttctg gatcgctcgc aaatcgtcat
    28261 gtggataact ttgtgcataa attatttaca tcgcttttaa tttattatca tgctttttac
    28321 agcatgatgt ttgtctttgt tttt
//
LOCUS       DAZFFG010000060        27523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFFG010000060 DAZFFG010000000
VERSION     DAZFFG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.060"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(136..882)
                     /gene="cutC"
                     /locus_tag="ACHJ7J_002828"
     CDS             complement(136..882)
                     /gene="cutC"
                     /locus_tag="ACHJ7J_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO5001042.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGISRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(896..1462)
                     /gene="yecM"
                     /locus_tag="ACHJ7J_002829"
     CDS             complement(896..1462)
                     /gene="yecM"
                     /locus_tag="ACHJ7J_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5001043.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1678..3411
                     /gene="argS"
                     /locus_tag="ACHJ7J_002830"
     CDS             1678..3411
                     /gene="argS"
                     /locus_tag="ACHJ7J_002830"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO5001044.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3453..3497)
                     /locus_tag="ACHJ7J_002831"
     CDS             complement(3453..3497)
                     /locus_tag="ACHJ7J_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001045.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3588..4076
                     /gene="yecT"
                     /locus_tag="ACHJ7J_002832"
     CDS             3588..4076
                     /gene="yecT"
                     /locus_tag="ACHJ7J_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO5001046.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHJ7J_002833"
     CDS             complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHJ7J_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO5001047.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHJ7J_002834"
     CDS             complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHJ7J_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5001048.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHJ7J_002835"
     CDS             complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHJ7J_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO5001049.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHJ7J_002836"
     CDS             complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHJ7J_002836"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO5001050.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHJ7J_002837"
     CDS             complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHJ7J_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO5001051.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHJ7J_002838"
     CDS             complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHJ7J_002838"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO5001052.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGATGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHJ7J_002839"
     CDS             complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHJ7J_002839"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO5001053.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11001..12602)
                     /gene="tap"
                     /locus_tag="ACHJ7J_002840"
     CDS             complement(11001..12602)
                     /gene="tap"
                     /locus_tag="ACHJ7J_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO5001054.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRVLQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12648..14309)
                     /gene="tar"
                     /locus_tag="ACHJ7J_002841"
     CDS             complement(12648..14309)
                     /gene="tar"
                     /locus_tag="ACHJ7J_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO5001055.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="ACHJ7J_002842"
     CDS             complement(14452..14955)
                     /gene="cheW"
                     /locus_tag="ACHJ7J_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO5001056.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAESEVA"
     gene            complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="ACHJ7J_002843"
     CDS             complement(14976..16940)
                     /gene="cheA"
                     /locus_tag="ACHJ7J_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO5001057.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDLLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16945..17871)
                     /gene="motB"
                     /locus_tag="ACHJ7J_002844"
     CDS             complement(16945..17871)
                     /gene="motB"
                     /locus_tag="ACHJ7J_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO5001058.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17868..18755)
                     /gene="motA"
                     /locus_tag="ACHJ7J_002845"
     CDS             complement(17868..18755)
                     /gene="motA"
                     /locus_tag="ACHJ7J_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5001059.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="ACHJ7J_002846"
     CDS             complement(18882..19460)
                     /gene="flhC"
                     /locus_tag="ACHJ7J_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO5001060.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="ACHJ7J_002847"
     CDS             complement(19463..19813)
                     /gene="flhD"
                     /locus_tag="ACHJ7J_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO5001061.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20593..21021
                     /gene="uspC"
                     /locus_tag="ACHJ7J_002848"
     CDS             20593..21021
                     /gene="uspC"
                     /locus_tag="ACHJ7J_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO5001062.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(21028..22452)
                     /gene="otsA"
                     /locus_tag="ACHJ7J_002849"
     CDS             complement(21028..22452)
                     /gene="otsA"
                     /locus_tag="ACHJ7J_002849"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO5001063.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(22427..23227)
                     /gene="otsB"
                     /locus_tag="ACHJ7J_002850"
     CDS             complement(22427..23227)
                     /gene="otsB"
                     /locus_tag="ACHJ7J_002850"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO5001064.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLASASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23394..24380)
                     /gene="araH"
                     /locus_tag="ACHJ7J_002851"
     CDS             complement(23394..24380)
                     /gene="araH"
                     /locus_tag="ACHJ7J_002851"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO5001065.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(24395..25909)
                     /gene="araG"
                     /locus_tag="ACHJ7J_002852"
     CDS             complement(24395..25909)
                     /gene="araG"
                     /locus_tag="ACHJ7J_002852"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO5001066.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(25979..26968)
                     /gene="araF"
                     /locus_tag="ACHJ7J_002853"
     CDS             complement(25979..26968)
                     /gene="araF"
                     /locus_tag="ACHJ7J_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO5001067.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
BASE COUNT         6749 a         7444 c         6595 g         6735 t
ORIGIN      
        1 ctgtcacccg ctcaacagat cgcggtaatc gttcattttt tatactatcc agggataaaa
       61 aaaggcccct gttgaaattg caggggcctg gtacgagcaa gcatcatatt gggcgacatg
      121 atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg attcctttca tttcagcaac
      181 cgccgccccg tctacgacat aacgcgaata ctcgtccgcg tgtgcatctg atgacatgga
      241 caatccttga ttacgataac gcatcggtga ggcttgccac gctcccgcgg agctatggac
      301 ttccagcact ccggcatcga ggaagtggtg caggttttct gcacggactc ctgctccggc
      361 cataatgatt ggagcatcac gatgggcaat aagttccata atttttgata aaccttgcag
      421 tgcgtctgat ttttgccctg atgtcagcac ccgggaaatg cccaattccg caagattatt
      481 gagtgtattt agagggttag cgcacatatc aaaggcgcga tgaaaagtca ctgccagcgg
      541 accggcagca gccattattt tttccattcg tggcatatcg acattcccgt caacatcgag
      601 aacgcccgtc accagtccag gaaaacctaa ttcgcggacc gtgcgcacat cctcaagaat
      661 ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg cgtgggcgga taatcggatg
      721 cacagggatc gtcacccgct ggcgcacgga tttcagtaca cccaacgacg gcgttaagcc
      781 cccctctttt ggggctgcgc ataattcaac tctgtctgcg ccgttttgct gcgccgttag
      841 tgcacattcc atgctgtaac agcaaatttc cagtaatgcc atttttactc cttaattacg
      901 ccgactgctc gctggcaacg atctcttcaa tggaccacgg atgaaactta atagttgttt
      961 tgccatcggt gaccgccagc gtcggattcg gtaatcgctc atgctcacct tttggcgaac
     1021 tggttttcac tgagatcccc ggtaggctga gtccttcatc agaaagcaat gccagcgcac
     1081 gggcattcag cgtttctgga tcacccggca gaacgatttc aatatgttcc caaccttcgt
     1141 gtgggtaacg tttttccccg ggccacggta gctccacaat agaaaactgc cagtgcgcaa
     1201 cctgtaccgg ttcatgcaat ttaaacagac aaatcggtct gccattgatc atattttctg
     1261 acaaaagctc gccacactgt tcaaacccgc gacgccagcg ttcagcagtg gcgttttgat
     1321 ggcaacgcaa agaaatgtga tcggcagtca gcggagtgat attcaacccc agacggcggg
     1381 aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga tgcaatatcc tgcagctcgt
     1441 cgatagattg ccagttcgcc ataatcactc ttcgtctttc agtaaaagcg ttaatttacc
     1501 ctgttgccct gcgccaacca accgctgatt tcacgccgct tctgatgcaa tagtgaaaac
     1561 ggcaatacgc cacgcgcacg ttgctgacga aaacagccat ttgcagtata ctcccgccct
     1621 aatttcttta actggtgcgg gcaatttttg ctcgcttcat caatgtaagg tattccggtg
     1681 aatattcagg ctcttctctc agaaaaagtc cgtcaggcca tgattgcggc aggcgcgcct
     1741 gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc agttcggcga ctatcaggct
     1801 aacggcatga tggcagttgc taaaaaactg ggtatggcac cgcgacaatt agcagagcag
     1861 gtgctgactc atctggatct taacggtatc gccagcaaag ttgagatcgc cggtccaggc
     1921 tttatcaaca ttttccttga tccggcattt ctggctgaac atgttcagca ggcgctggcg
     1981 tccgatcgtc tcggtgttgc tacgccagaa aaacagacca ttgtggttga ctactctgcg
     2041 ccaaacgtgg cgaaagagat gcacgtcggt cacctgcgct ctaccattat tggtgacgca
     2101 gcagtgcgta ctctggagtt cctcggtcac aaagtgattc gcgcaaacca cgtcggcgac
     2161 tggggcactc agttcggtat gctgattgca tggctggaaa agcagcagca ggaaaacgcc
     2221 ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg atgcgaaaaa gcattacgac
     2281 gaagatgaag agtttgccga gcgtgcacgt aactacgtgg taaaactgca aagcggtgac
     2341 gaatatttcc gcgagatgtg gcgcaaactg gtcgacataa ccatgacgca gaaccagatc
     2401 acttacgatc gtctcaacgt gacgctgacc cgtgatgacg tgatgggcga aagcctctac
     2461 aacccaatgc tgccaggaat tgtggcggat ctcaaagcca aaggtctggc agtagaaagc
     2521 gaaggggcga ccgtcgtatt ccttgatgag tttaaaaaca aggaaggcga accgatgggc
     2581 gtgatcattc agaagaaaga tggcggctat ctctacacca ccactgatat cgcctgtgcg
     2641 aaatatcgtt atgaaacact gcatgccgat cgcgtgctgt attacatcga ctcccgtcag
     2701 catcaacacc tgatgcaggc atgggcgatc gtccgtaaag caggctatgt accggaatcc
     2761 gtaccgctgg aacaccacat gttcggcatg atgctgggta aagacggcaa accgttcaaa
     2821 acccgcgcgg gtggtacagt gaaactggcc gatctgctgg atgaagccct ggaacgtgca
     2881 cgccgtctgg tggcagaaaa gaacccggat atgccagccg acgagctgga aaaactggct
     2941 aacgcggttg gtattggtgc ggtgaaatat gcggatctct ccaaaaaccg caccacggac
     3001 tacatcttcg actgggacaa catgctggcg tttgagggta ataccgcgcc atacatgcag
     3061 tatgcataca cgcgtgtatt gtccgtgttc cgtaaagcag aaattaacga agagcaactg
     3121 gctgcagctc cggttatcat ccgtgaagat cgtgaagcgc aactggcagc tcgcctgctg
     3181 cagtttgaag aaaccctcac cgtggttgcc cgtgaaggca cgccgcatgt aatgtgtgct
     3241 tacctgtacg atctggccgg tctgttctct ggcttctacg agcactgccc gatcctcagc
     3301 gcagaaaacg aagaagtgcg taacagccgt ctgaaactgg cacaactgac ggcgaagacg
     3361 ctgaagctgg gtctggatac gctgggtatt gagactgtag agcgtatgta atcgattttt
     3421 cgtgagcgtg aagcctgatc agggttagcc gatcaggctt ttttattgcc atctaaatgt
     3481 attctgaaaa cggacatgcc atcgttttct cactgttgag taagaggcca gaagcgtaat
     3541 atccaggccc aggaaaacga taacggttca atttaaggaa taccgcagtg tttaaatttc
     3601 ttgtattaac attaggcatt atctcttgcc aggcttacgc agaagatacg gttatagtaa
     3661 atgatcatga catttcagcc atcaaagatt gttggcaaaa aaattcagat gatgatactg
     3721 acattaacgt gatcaaatca tgtctgcgac aagaatacaa cctcgtcgat gcgcaattaa
     3781 ataaagctta tggtgaagct tatcgttata tagaacaagt gccacgcaca ggtgcaaaaa
     3841 aacctgatac cgaacaactt aacttgctta aaaaatcaca acgagcctgg ctggatttta
     3901 gggacaaaga atgtgaatta atcctttcaa atgaggatgt tcaggattta agtgaccctt
     3961 attctgaatc agaatggcta tcatgtatga tcatacagac caatacgcga actcgccagt
     4021 tgcagctata ccgtaactct gaagattttt atccaagccc tttgacaaga ggataattca
     4081 catctttttg gcatgttttg ttgcaagcta ttcctgataa ataattgcaa caaaacatcg
     4141 agcctttttc gctgagttat taaacatgct cgcgagcgcg taatttttat ttgtccttca
     4201 gcgataattc acaatcactt cattccgttg tacctttagc ggtggaatta accgcccacc
     4261 gccaggcact tcccagataa agcgcaacgg ttctgctgcc gctataccgg aaaaggccac
     4321 ggtggttccg ctctgcccct ctaattcgac acaacgtgat tgcgaacaca agcgaacccg
     4381 cagtcctgca ggtgtcgggc cgataagctg ataacgccac gccaccagcg tcatcaatcc
     4441 tgaagcaggt tgtcgagtag aaagaggcgc tgacgacatc gactcaccgc gatgatttag
     4501 cgtaattccg acactacttg cctgccacat cccctccccg gctgcttgca ctagcagcgg
     4561 aaacaataat attgctaata aggttctcat catttaccgc caattgtcgc cgtcatgcgg
     4621 atatgtcggt tatcagatag ttccagattc gacagcacca ctaactgcgg caagctgcgg
     4681 cgcagaaagc gagacaataa tggtcgcagc gcgtggttca ccaacaatac tggcggcgca
     4741 cccaacatct cctgacggga tagcgcttcc tgagtttgcg ccagtaaacg atccgccagc
     4801 cctggctcca gtccgccccc gccctgcaac gcctgtagca acaaacgttc cagcggtgta
     4861 tcgaggccaa taacatggac ttcatctttg ccaggaaacc actgctgggt aatcgcccgt
     4921 cccaacgcca cgcgcacgac ggcggttaat tcatgtggat cactttggat gggtgcatgt
     4981 tccgccagcg tttcgagaat agtgcgcata tcgcgaatcg gcactttttc atcgagcaga
     5041 ttttgcagca ctttatgcag tgtggtgagc gtgacgacgc caggaacgag atcttccgtc
     5101 agctttggca tctcctgggc gacgcgatcc agcagctgtt gcgcctcctg acgaccaaac
     5161 agctctgcgg catgctggct aatgaggtgg ttaagatgcg ttgctaccac cgtgctggcc
     5221 tcaaccactg tgtacccctg aatctgcgcc tgttctttta gcgcactttc aatccagata
     5281 gcattcaggc caaatgccgg atcgacggtc gcctcaccag gtaacgtccc ggcagcggta
     5341 ccagggttaa ttgccagcca gcgccccgga taagcatcac cactgccaat ctctacgcct
     5401 ttcatcaaaa tacgataacg tgccggttgc agatccatat tgtcgcgaat gtgcaccacc
     5461 ggcggcaaga agcccatttc ctgagcaaat ttcttacgaa tactacgaat acggcccagc
     5521 aactcgccat cttgttggaa atcaaccatc gggatcagtc gataacccac ttccattccc
     5581 agagaatctt ccagttgtac atcgttccac gtcgcttcga caacggtatt attctctgcc
     5641 atttttaccg gtttgggttc ggcaggcgct ttttgttcgc gtccgcgtat ccaccaggcc
     5701 agcccgagca atccggcagt gaacagcaaa aataccaggt tcggcattcc aggcaccagg
     5761 ccgagtaaac cgagcacggc ggcgcttaac aacataacgc ttgggttact gaaaagctga
     5821 ttcaccatct gctcgccaac atcctgatcg gtgctgacac gcgtaacgat gacccccgcg
     5881 gcggtagaaa tcaccagcgc cggaatttgt gccaccagac cgtcgccaat ggtcaatagc
     5941 gtataacttt ccgccgcgtg tcccatgctc atgccatgtt gcagcacgcc gaccagcaac
     6001 ccgccgacaa cgttaatgac catgatgagg atcccggcga tggcatcgcc gcgaacaaac
     6061 ttacttgccc cgtccattga gccgtaaaaa tcggcttcct gagtcacttc ggagcggcgt
     6121 tttttcgcct catcttcacc aatcaatccg gcgttaaggt cggcgtcaat cgccatctgc
     6181 ttacccggca taccatcgag aacaaagcgc gcacccactt ctgcgatacg cccggcacct
     6241 ttggtaatga ccataaagtt gatgatcacg agaatgacaa acaccacgat accgatagcg
     6301 aaattgccac caacgaggaa gtgaccgaac gcttcgacca ccttccctgc cgccgctgcg
     6361 ccggtatgcc cttccatcaa aatgatacgg gttgaagcca cgttaagtgc cagacgcaac
     6421 agcgtggtaa acaacagaat ggtcggaaac gcagcaaact cgagcgtgcg ctgggtaaac
     6481 atcgccacca gcaacaccat gatcgacaag gcaatattga aggtaaacaa caggtcgagt
     6541 atgaatgcgg gcagtggcag caccatcatc gacaagatca acaggatcaa aatcggtccg
     6601 gcaaggatct gccattgtgt cgatttcagg tttgcgggca ggcgcagcat cgcggccaga
     6661 ttactcatgg gtcggtttct cgttaataaa atccagggct tccggcaccg gaagatgagt
     6721 aggttgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     6781 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     6841 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     6901 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     6961 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     7021 atccgctttc ggcacatcgg ccatcatccg acgccgtgca gccgctcgct gcatctgacg
     7081 gatccgccct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
     7141 ccgcgacata cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     7201 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccagat ccatcgcatt
     7261 acccatggcg gtaatcggag actcggccat taagcgcatc atctgcggcc agtgatgcca
     7321 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgctt taagtaactc
     7381 cgcgccagtc tgtgccgaga acatccgttt aatgcccggt agcgggttga gtttggaaaa
     7441 cttcggctgc aaggatttgc cgctaaatac cagccctccc agcatgaccg gagaaatgag
     7501 cgccaccagc accacgccgc taatcagcgg cagcagcgcc agcatggctt ctctgatcag
     7561 cagaataatt tgcccgagga tcagattcgg gtcattgata atactgtgat caaaatgcag
     7621 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     7681 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     7741 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     7801 gtcagacacg tcgccaatcc tggaaagagt attaagcgtg aatgatgcca gagcgcaaag
     7861 cgttcaatgg tttgagtaag gggcaaaaca ggcgggattt agggcttttg ctgccacaca
     7921 tcaaacatag tgtgcgtttg tcggatgcgg cgtcatcgcc ttatcagacc gcctgatatg
     7981 acgtggtcac gccacatcag gcaatacaaa tcaaaatcca agactatcca acaaatcgtc
     8041 cacctgatcc tgactggcta ccacaccggc tttgctggta tcgacctgag gtccattaag
     8101 caaactctgg ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt ccaacagcac
     8161 catcagcaac tggcgttcga tctcctgaat gacatccatc atccgcttaa tgacctgccc
     8221 ggtgagatcc tgaaaatcct gcgccatcat gatttccagc agttgcgcgt tagtaaagct
     8281 ggtatgcgcg ggtacatctg ccagaaattg tcgtgtatct gttaccagtt cacgggcgtc
     8341 ggcaaggtca atcggatcgg caaaccagtc atcccaacgt tgggttaacg cttttgctga
     8401 tttctccatt tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg cccgctccgc
     8461 agcctgggcg gtcatctgca caacatagta caaacgatcg cgcgcatccg ggatggcttc
     8521 cgccgcttcg gcaatggcct gatccagccc cagttcccgc aaactgtcgc gcagcatacg
     8581 cgtcaggctg ccgatgcgcg caatgatatc gccagctgaa tgctcgtcag caggtttgat
     8641 tgatggttgc atcatagtcg catcctcaca tgcccagttt ctcaaagatt ttgttgagtt
     8701 tttcctccag cgtcgcggcg gtaaatggct tcaccacata gccactggcc cccgcttgcg
     8761 ccgcagcaat gatgttctct ttcttcgctt ctgcagtcac cattaacact ggcaatgctg
     8821 acatcgcgcc atccgcacga attgttttca gcaattccag gccatccatg ttgggcatgt
     8881 tccagtcgga gataacaaat ccataaccgc ctgcctgcaa cttattgaga gcgtcgacgc
     8941 catcttccgc ttcctcaaca ttattgaatc ccagctcttt cagcaggtta cgcactatgc
     9001 gtcgcatggt ggaaaagtca tccacaacca aaaatttaag ttctttatcc gccatttcac
     9061 actcctgatt taaatacgta tcgcctgtcc ggcactaatt tttgccaaca tttgctggct
     9121 tacctggcta agatcgacca cttcgcagac accacccata ttgatggcct cacgcggcat
     9181 gccgaacacc acgcaacttg cttcgttttg cgcaagggtc catgcccccg cctgacgcat
     9241 cgccaacatt cccgtcgcgc cgtcgttgcc cataccggtc aggatcaccc caaccgcatt
     9301 acgccccgcc tgtttggcga cagaatggaa caacacatct accgaaggcc gatgacggtt
     9361 aaccgccggg ccatcgtgaa ttttgatttg gtaatttgcg ccactacgcg ccagctccat
     9421 atgccgatcg cccggcgcta tataagcatg tcctggcaag acacgctctc cgtcttcggc
     9481 ttctttaacc ccaatctggc aaagcttatt aagtctgtcg gcaaaagagc gggtgaaacc
     9541 tggcggcata tgttgggtaa ttaacagtgc cgggctggaa agcggcaatg gttgcagtac
     9601 gtgacgaatt gcctcagttc cccccgttga agcaccaatc gcaatcagtt tttcagaact
     9661 caacaacggc cccgccttca gcgttgtcgg tgccgacaat ggcttatgtg ctgcaaggct
     9721 cgcctttgct gctgtacgca ccttttcagc aatcatttcg ctatacgcca gcattccttc
     9781 gcgaataccc agttgcggtt tggtgacaaa atctatcgcc cccagctcca gcgcgcgcag
     9841 cgtgacttct gaccctttgc cggtcaggga agaaaccatc acaacgggca ttggacgcaa
     9901 acgcattaat ttttcgagga aatccagtcc gtccatccgc ggcatttcaa catccagcgt
     9961 caatacatca ggattgaatt tcttaatcaa atcacgcgca accagcggat caggggcggt
    10021 cgccaccatt tccatgtcgc tatggctgtt gatgatttct gtcatgatct ggcgcatcag
    10081 tgccgaatca tcgacagata acaccctgat tttgctcatc gttaatcctt acttagcgca
    10141 tacaccgtct gaccacgcag cgtgaagcgg cgctcgaggt ggctaaagtt ttcagagtga
    10201 cccgcaaaca gcaatccgtc gggtttaagg agcggaacaa agcggcgcaa aatctcctgc
    10261 tgggtagttt gatcgaagta gatcatgacg ttacgacaga agatcgcatc aaacggcccc
    10321 ggcacggtgt actgtttcgc cagtaaattc agcggggcaa aatcaacata gttcgccagc
    10381 tcctgacgca cgcgtaccag cccttcatgc ggtcccgtcc ctcgcatgaa ataccgttgc
    10441 agttgctgcg gcgtcaggtt tttcaactct tcatggcgat agataccgct tctggctttt
    10501 tccagcactt cggtgtcgat atcactggca aacactttcc agcgtccggg cgcggtgccc
    10561 aatgtgtcag ccagcgtcat cgcaatgctg tacggctctt cgccggtcga agccgccgcg
    10621 ctccatacgc gatactcgcc agaacggcga cgtgcgtgat ccgcgagcag agggaaatga
    10681 tgtgcctcac ggaaaaatgc cgtcagattc gtggtcagcg aattgataaa cgcctgccac
    10741 tcaccgctgt gctgattaga ttccagcaag ttcagataat gaccgaaatc cgtcagtccc
    10801 agcgaacgca aacgacgaac cagtcggttg taaaccatgt cgcgtttatg gtcagccaga
    10861 acgatcccgg ctcgttgata gatcaattga cttatccgcc gaaaatgcgc gtcggaaagc
    10921 gccaggcgct cggtcatctg taacaataaa gacgtttgcc cacagggcag agatgaagtc
    10981 atagcgcctt ctcaatcact tcaggatacc actggcgcaa tttgtaactg cgccgactca
    11041 tgtcgtgcta cttcatgttc ttcaagggta aataccgcca cacgcgacga aagatggtcg
    11101 gcctgattcg ccagttgttc cgttgccacc gccgcttctt ctaccagcga ggcgttctgc
    11161 tgagtcacct gatccatctg gctgacagcc tgtgcaacct gctcaatccc ccgccgttgt
    11221 tcttccgacg ccgaagcaat ttctcccata atgtcgttca cgcgggtcac cgaactgaca
    11281 atatcggtca tggtcgcggc ggcgttattc accagtttcg aaccctgctg gacacgattg
    11341 actgactctt cgatcagccc tttgatctct tttgctgcct gcgcgctacg gctggcaaga
    11401 ttgcgcactt cacctgccac taccgcaaaa ccacgcccct gctctccggc gcgagccgct
    11461 tccactgccg cattcagggc cagaatattg gtctggaaag caattccgtc gataacgctg
    11521 ataatgtcgc caattttttg cgagctggtg gcgatctcct gcatggtgtg agtcatggta
    11581 ctgacctgaa caccgcccgc ctgcgccgtt gtcgcggcat tttttgccag ttccgacgcc
    11641 tgtcgtgcgt tatcggcgtt ttgccctacc gtggcggtta attgctccat actggcggcc
    11701 gtttgtgcca gcgatgccgc ctgctgttca gtacgacttg agagatcgtt attgcctgcg
    11761 acaatctccg cgatcccaat gtgcatctcc tggcttccct tacgcacatc actcaccgtc
    11821 ccacgcaaag cctgctgcat ggtcttcagg ctggcaaaaa tggcggtgat ctcattacga
    11881 ccatataccg caatcggacg cgccagatta cccgcagcaa tgctgtcaaa atggctaccg
    11941 ataatggcca gtggttgaac aatcatcttg cgcgtccacc acagcgcact gctgatgtag
    12001 atcgccgcaa caataatcat gctgataaac accagcgccg aaatctgata gttacgctgg
    12061 ctttgcgcac tggcggcttc cagcacatgg ttgatctcca gctgccaggc ctcaaagtta
    12121 acgtcaaacg cattctgtga tccctgcacc ggcgcagtga ggaaatccga aagttgatta
    12181 ctttcaagcc aggtcgcctg atgttcgaga tcgttgtgcc agcgggcaaa acttttctcc
    12241 gtctcttttt gcaataccct gacgtgctcg ttgcccgcag tcatcgccat aaaactttta
    12301 aacagcgtgg tggattgcgt cagactggcg cgcgccgtcg tcatcaacgt tttaatgtca
    12361 tccgccggat agctaagcgc tgtcagagtg cctgctttgt tcagcgcggt actggcctgt
    12421 aacattaccg cccgagtttg cgctaatgcc gcacgttgct gattgctctg ctcgacctga
    12481 ttcagtcgtt gcaaatcgtc gcgaaatgcc caaaaagaca tgccgttact gccaatctgc
    12541 aagatcccgc agagaatcaa aattaaaaac agcgtggtcg aaattcgaat acgattaaac
    12601 atcaacgctc cccatcaggc ggcaatgacc gcgttagtaa atactcgtca aaatgtttcc
    12661 cagtttggat cttgttcagc aattcgcagt cgtggctgtg ccggtggttg ctcactggca
    12721 ggacgggatg gtgtttgcgg tttattggtg agtgggctgg ctgccagacg aaacgcggaa
    12781 accgcttgcg ttaaacgact agcctgttct tccagcgcag ctgcggcggc agctgattcc
    12841 tgcaccagcg atgcgttctg ttgcgtgacg cgatccattt ccgaaaccgc caatgcgact
    12901 tgatcgatgc cacggctctg ttcatccgat gccgatgcaa tctcgcccat aatgtcagtc
    12961 acgcgagtga cagcattgac gatattgttc attgtttccc cggcgctttc gaccagcacc
    13021 gaaccggtat caacgcgcga gacggagtct tcaatgaggg ctttgatctc ttttgccgcc
    13081 tgggcgctac gactggcaag attgcgcact tcgcccgcca ccacggcaaa accacggccc
    13141 tgttcacccg ctcgcgcggc ttcaaccgcg gcattcagcg cgaggatatt agtctggaag
    13201 gcaataccgt cgataacgct gataatgtcg gcaattttct tcgaactatc ggcgatctca
    13261 tgcatcgttt tcactacgcc atccaccact ttgccgccgt gctgggcggt gtcggaggca
    13321 ctttgcgcca gttgcgaggc ctggcgggcg ttatcggcgt tttgcttcac tgtcgcggtg
    13381 agctgctcca tgctggcggc agtttcttcc agcgcggatg cctgctgttc agtacgggag
    13441 gaaagatcgg tgttgcccgc cgcaatttca cgggtaccgg catagatggc atctgaacct
    13501 tcgcggacat gagtgacggt gtcagtcaaa gagcgttgca tatgtgaaac gctctgcgcc
    13561 aggtcgccca tttcactgcg cccgtcaatg gtcagggtat tcgccaggtt accaccggcg
    13621 atttcgcgaa tgtgagcaat aatttttgcc agcggtgtaa gcaacatacg gcgaatgccg
    13681 taccacgcca ccagcagaat caataccacc accagcgcga taaccgccag ttgccactgg
    13741 gcaaatcggt aatcatctgc gttgtcagtg acgatatcgc gatacagttt ttcactgcta
    13801 agggcgtact gagcaaacgc ttcgcccatt gcattttgca ttccctgggt tggctgagcg
    13861 aaataagctc cagtattgcc ataatcaaga taatcaatca gttcagttaa cgctgtgtaa
    13921 tagtttttat atttttcatc aatattacga ctggtagcga ccatttcagg taacggtgcc
    13981 atgcttttga attttttata atgagtcgcg gcctgggcca atgttttcct ggcgctatcg
    14041 agcaattcaa ctttggcgtt actttgttga ttggaggaat ccatcatcat ccgcaccgct
    14101 gaacgactca ggttaatgcg cgtttgcagc attaaatccc aggttgacgt cagctcgccc
    14161 tgctgttccc gtaattgatt ggaaaccaca aagctcttct ggctatggtg aagggaagaa
    14221 aaaaacagac tgccggaaat aagctgtaac agtgcgaata cccccagcac cattaccaac
    14281 agcgtgacta cgcggatacg gttaatcata aggcaccttc ctgaaaacaa gttgatctcg
    14341 ttatcggcaa gaagggggga aactttattg ctgatgccac ccgccgcgaa attgaaataa
    14401 aacccgatgc gcagatcatc gggttcattt caattgagga aatcaggaga attacgccac
    14461 ttctgactcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt tttcgatgtt
    14521 caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt ctgttgaaag
    14581 cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa gcacgtctga
    14641 aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga taactaccgt
    14701 gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa caatcggcac
    14761 aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg caatccgtgt
    14821 tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt actcttcatc
    14881 accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct ttgttacatt
    14941 cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt cgccatacgt
    15001 tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac gctgccgtcg
    15061 ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc aaggttttta
    15121 actacaacct gatgttgacc aattaattga tccaccagca aggcgtagcg gcgaccgcca
    15181 ctttgtaaga tcaccacaat tccctgggta gcttcggttt tcgcgcccgc gacgttgaat
    15241 actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac ccgctcgccg
    15301 ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac agcattcagc
    15361 ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat ggccagcgtc
    15421 agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat ttcaacatga
    15481 ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg cccggagacg
    15541 tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcatcgc gacttcgtcg
    15601 tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag aattcgctca
    15661 cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc ctgatgttcg
    15721 gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt ttctggcagt
    15781 tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcaggtcgat aatgcgttct
    15841 atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac ctgcttgccg
    15901 agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata ttccatcggc
    15961 atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg taactgcccc
    16021 atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg ctgggcaagc
    16081 atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac cttttctacc
    16141 gctacacgga tgctggtgga ttcactgcta cgcgtcgttt tttcccgctc cacgcgaccg
    16201 gttggcgctt gttcggctgc cagtttaagc actggtgggg tggatatttt tggcgagact
    16261 tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac cgctgtgatg
    16321 tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc tttcaccacg
    16381 tcagttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc gctggccttc
    16441 aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tctgctcatc ttgcggttca
    16501 cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt cgcttctaat
    16561 gccagttgac gcaaggcctg gcagatataa tcgaagctgg cggcatccgg ctcttgcgac
    16621 tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa cagattgata
    16681 atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt ttccatcaga
    16741 tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt gatcgagtgg
    16801 gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg caaaaccagc
    16861 agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt ctgataaaaa
    16921 tcgcttatat ccatgctcac gctgtcacct cggttcggct gatggcattg tgggaacact
    16981 gacctgtggt gcaacctcag gtttttccag ggcgcttact ggctcattct ggctttcggc
    17041 gttttcatgc aaaatggcct gttcggcttg tttgttcagt accagcaggc tgatgcgacg
    17101 gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca tgccgaccac
    17161 acgtaacact ttgccgctat ccaacccgcc gaccatcagt tcgcggcggg atgcattggc
    17221 ccgatcggca gaaagctccc agttgctgta acctttctca ccactggcgt aggggaaatc
    17281 atcggtatga cccgaaaggc taatacggtt gggaatacca ttcagaacgg gcgcgatggc
    17341 acgcagaatg tcgcgcatat agggttcgac gtcggcactg ccagttctaa acatcgggcg
    17401 attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga ttttgagatg
    17461 tggacgtaac gcccgcagtt tcggatcgga ctctatcaat tggtcgagat cgccacgcaa
    17521 tttccgcaat cgactttgct ccatgcgttt tttcaactct tcgatgttcg gctgcttatt
    17581 cacttccccc tggctttggg tgtaatcatc gccaccgccg ggaattgggc tttcactatt
    17641 agaaatgcga tcgccgcccg taaccgcagt cgccagtgga gtccggaagt actccgcaat
    17701 ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa aaaaggccat
    17761 catcgcggtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg ccccgtggct
    17821 tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca tgcttcctcg
    17881 gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag ttcaataaac
    17941 gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat aggcggtgcg
    18001 taaccgttca gattagaaag cagtgtgact ttgacgcact gcatcatttt gctggtttcg
    18061 gcgcttttct gacgtaaaac actcgctaat ggggaaataa atccgtaagc caataaaatg
    18121 ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc ggcaggacga
    18181 tcggctgaac ctaacgcgtg aacgaccccc attacagccg cgacaatacc aaacgccgga
    18241 agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct ttcgtgcgtc
    18301 tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc gctgataatc
    18361 aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat gcgtgggtag
    18421 ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga aaacatcccc
    18481 atctgccgcg atttcgccat caaccgataa agcagcgcca gcagatccat atacattgct
    18541 ttggtgtatt tggagcgacg aaacagcaac ggcagcgcct tcagcgtgcc tttaatcgct
    18601 ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat caccagttca
    18661 gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc gaaaactgta
    18721 ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca ctgttgacca
    18781 tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggcaaaaaaa agcagcggta
    18841 cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc ctgtactctc
    18901 tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt acgtcttttt
    18961 actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg ctggtgagcg
    19021 tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa tccactttca
    19081 acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gcggtccttc ttctgcttgc
    19141 gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc attacacaaa
    19201 ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac gttttgttcc
    19261 caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg gcttccgcgc
    19321 agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct ttccagcatc
    19381 tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg cgcttcctga
    19441 acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct tcaggctgat
    19501 taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc tggagatcgt
    19561 caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa cggaagtgac
    19621 aaaccagctg attggtttct gccagcttaa ccatttgcgg aagagtcagt gccgctaatg
    19681 ttgtcgccat ttcttcattt atgccgagac gaaacatagc ggacgctttg tcctgaacaa
    19741 tcagacgctg tgcaagtagt aaatatgaca agttgatgtc ataaatgtgt ttcagcaact
    19801 cggaggtatg cattattccc acccagaata accaacttta tttttatgcg gtttcaccgc
    19861 accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa tagttgcgat
    19921 aagctgcaat aagcagagcc acctttttgg tttaataagt ccttacaaat agaagggggt
    19981 ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca ctacacgcac
    20041 atacaacgga ggggggctgc gattttcaat aatgtgtgat gcagatcaca caaaacactc
    20101 aattacttaa cataaatgta taagtcatac ttttgttttg ggtgtatttc aatctgttaa
    20161 aaagtttttc gctacgctag caagcaaaaa tgaaacagga ataatcgaaa tgggatgttg
    20221 cgcacagtta aaataactca ccgtaaataa tcatctgcta taaataatca ctttcatgca
    20281 ataccagata agctattttt aaacagacac ttaccgcaca acaaactaat taaatacaaa
    20341 gaaaggcaac aatcaactta atgttaataa aatgttttgc atatattaac cacttgttta
    20401 atgcggggaa tcagtaaatt attttcttat aattacagtt gaaatcatat gatcaccgag
    20461 agtgattgat taattgtctt tactaataat cagactaata tttacctgtt tgactgagtt
    20521 gggattgcgt cgtttctcca ttaggagtaa agctttaatg tcacctgaag ttcacagaat
    20581 aaggaacagg ttatgagcta tagcaatatt cttgtcgctg ttgcggttac accggaaagt
    20641 cagcaactgc tggcaaaagc ggtatctatc gccaggccag taaagggaca catcagttta
    20701 attactctcg cttccgaccc ggaaatgtac aatcaattag ctgcgccgat gcttgaagat
    20761 ttacgcagcg tgatgcagga agaaacgcaa agctttcttg ataagttaat tcaggatgcg
    20821 gggtatcccg ttgacaagac gtttattgcc tacggtgagt taagcgaaca tattctggaa
    20881 gtttgtcgca aatatcattt cgatttggtg atttgcggta atcacaatca cagtttcttt
    20941 tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa gtgaggtcga tgtgctgtta
    21001 gttccactta cgggagatta accgctccta cgcaagctta ggaaaggtag caactttatc
    21061 gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt aggtcgctaa tgaagcactc
    21121 ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc atttctgcat gacgggaaat
    21181 acgttccgcc agcgacatag tcaatgcacg atccagcgca gctgcaactt cgtcacgatc
    21241 gtagggatta acaattaacg ccgacgttaa ctcgtttgcc gctcccgcaa attgcgaaag
    21301 aacaagaacg cccggattgg ctgggtcctg agcggcaaca tactcttttg ccaccaggtt
    21361 catcccgtca cgcagtggcg tcactaagcc cacgtcagag tagcggaata ttttcatcag
    21421 taatttacgg tcaaaatgct gattcaaata ataaagcggc gtccagccta attgcccgta
    21481 tttaccatta attcgtccgg cttcattttc gagctgatga cgaatatcct gataggcttg
    21541 cacatcacca cgcgacgttg gtgcaatctg ggtataacga attttaccat gatgctgcgg
    21601 atatttttcc agcaacgctt cataggcgag aaaacgctct ggcaaacctt tggaataatc
    21661 cagccgctcg acagaaaaga tattttgtac gtttttcagt tccgctttaa gttgcgccag
    21721 ttttggcggc agtggcccgg cagcctgttt ggctatttct tttggctcaa tgccaatcgg
    21781 gtaaacttct gttcgaaacg ctttgcccca ggctgtatgg ctttttgcgc tacgtgtcgt
    21841 gacgcgggtc aggttagaaa ggcaatccag gaacgccaga cgatcgtttt ctgtctggaa
    21901 acccagcaaa tcataatcac aaagctgttc aagcaaggtg tcatatgtcg gcagcgcgtt
    21961 gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa ccaatgcgat tattcactcc
    22021 ccgtttgcgt aattcatgcg caaatggcaa caggtgataa tcgtggatcc agataatgtc
    22081 atcgtcttgc aacagcggca gtaatttatc tgccagcaac gcatttacgc gtagatagcc
    22141 gtcccaggca ggacgctgaa attgcaccag atcgagccga taatgaaaag cgggccagag
    22201 aacggcattg gagaattggt tgtagtattc gtcaaggtcc tgttcgctga ggttaaaaga
    22261 ggcccacgta atgttacctt ttttcacctt ttttagcggc tgatcctcat tccctgtttc
    22321 accactccag ccaaaccaca gtccgcctgc ggctttcagt gcccccagta tgccaacggc
    22381 aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca atccggttag atactacgac
    22441 taaacgactc atagtcatca ctcctgttat tttctctttt ttgttgtaat gcggtggtta
    22501 tcatttcaag ccagctccag acatccggta cgcccgccag tcgccatgat gcctgagttg
    22561 cgcctgtgcc aatttttact gacattccgc ccagccggtt aacgactgcg aagccagatt
    22621 catcggttaa atcatcgccc agaaatacgg gcgttcgccc gataaaggga gcttcctgca
    22681 taaaggcagc aatcgcctca cctttactgg tacctctcgg tttgatctcg acaacacact
    22741 ttccctgctg taacgccatt tgtggccaga tctgagtaat acgttgcgct aatgtcatta
    22801 atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc aaaagccatc cctttcgtct
    22861 ccagttccgc accgggatac tgggcgatga ccgtatgcag ttgcacgcta atatcacgcg
    22921 caatcgcatc cggcagatga acgatatgcg ttttaccatt gatgtcacgg cgctccgccc
    22981 catgcacgcc cgctaacggg aagcgataag gttttgccag tgcgtccagc tccaccattg
    23041 agcgccctga tatcaatgcc aatgcaccat cacttgcgct tgccagtagc tgaagtcctt
    23101 gcagaatatt gtcaggtacg acgacctgat cgggatgcgg tttgatttcc gccagcgttc
    23161 catcgagatc aaaaaaccag gcatatttcg cggatagttc aggggtttcg gttaacggtt
    23221 ctgtcacccg gttctcctcc ttctcttttc ttattgtgtt gcgcagaacg cgctttacag
    23281 acatgtaagt atagacagtg tgacgggggt cgccatttgc aacgaagaac agccaaccgt
    23341 tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt gtggaaaaaa gcatcagaca
    23401 gtgcgtttcg ctttttgctt gtaacggtcg aagatcaccg ctgccagcag gattaagccg
    23461 cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt tcatggcgtt ttccacggtg
    23521 cctaaaatta agatacccgc caccacatat gagatttttc cgatgccacc tttcagagaa
    23581 acgccaccta aaacgcaggc ggagataaca ataagctcat aaccaatcga tgtcattggc
    23641 tgcccactag tcatacgtga agccagaata attccggcta tcgcagatac caggccagag
    23701 agaacaaaga taataatttt ggtgcgaaca accggtacac ccgccagacg cgcagcctct
    23761 tcgttcccgc caatcgccag ggtattacga ccaaaggtgg ttttattcag cagcaaacca
    23821 aagataatca gacacgcgac ggtgagccag attggcgcag gcagaccgaa ccagttagcg
    23881 taaccaaggg caaagaagct ttcatcttcg ataccgaccg ctttaccgtc tgaaatgatg
    23941 tacgccagac ctcgaacaat ctgcatcgtt gccagtgtcg tgatcagagc atttattttc
    24001 agtttggcga taacaaagcc attgaccagg ccacagagaa tgccaagcag caaccccgct
    24061 gccacgccaa tccacaggct ttcagtcagg ttgataacca ccgccgtggt gacacccgca
    24121 caggcaatta cggaggcgac agaaaggtca aagtcaccgg aagcaaggca gaacaacatg
    24181 ccacacgcca ccatccccga catggaaatt gccaggccca accctttcat attaataaag
    24241 gtggcaaagt tcgggacaaa aatggcacag gcgataaaaa gcaccgcaaa caccaccagc
    24301 atgccgtact gatcccagat acgcccgaag ctgaatgacg acttaggtgc gccagaaccc
    24361 gatgtagaaa cagaagacat catactctcc ttactcaggc aactgcctgg ctgactttag
    24421 gcatcgcaag gctcagtgcc tgacgctcat ctgcctgctc gtgtaacaat tcaccggcga
    24481 tttcaccttc ccgcatcacc acaatccggt cggcaacgcc gaggacttca ggtaagtcgc
    24541 tggaggcaaa cagcaccgcc acgccctgcg ccgccagcgc ataaattacg ttatatattt
    24601 cgtgcttagc gccaacatca atgccgcgcg taggttcatc cagcaaaatg accttcatct
    24661 cttccgataa ccagcggccc agaatggctt tttgctgatt tccgcctgag agattcatga
    24721 tcagttgctc cgcgcccggc gttttgatgt tgagcgaacg aatgtggtga tcggcattgt
    24781 tttcttccca accgttgttg attacacaac cgccgagcac atgtttacgt ctggcactga
    24841 tgttgatatt gtcgcgaacg gagtgcacgg gaataatgcc ttccgctttg cgatcttccg
    24901 ggcagagcat catgcctgcg gcaatggcgt ggctcggttt acgaatatcg atcggctgtt
    24961 ggtcgatata aacctgaccg gcggtgattt gcgtcccgcc aaacatgcct ttcattaatt
    25021 cgctacgccc cgcccctacc agaccaaaca gcccaacaat ttcaccactg cgaaccgcca
    25081 gacttattgg cgtacgcacg cctggtgctt tcacagcatc aagacgcagg cgctcctcgc
    25141 cataactacg cggttgccag ccgtagatat cgccaatgtc gcgcccgacc atcgcctgca
    25201 ccagcgcgtc gtggtcaacc tgctgcatat cggtaaaggt tttgacataa cgtccatctt
    25261 taaagacagt gatggcatcg ctgagggcaa atatttcttc catacggtga gaaacgtata
    25321 agatcacccg cccctctttt cgcagttcac gaataacgcg gaaaagattg tcgatttcgc
    25381 gggcagaaag ggagctggtt ggctcatcaa aggcgataat tttagcgtta cgcgccagcg
    25441 ccttggcgat ttcaaccatc tgccactgac caatggagag atatttcagc ggcgtgtccg
    25501 ggtcaatatc cataccaaga tgtttaagtt gtaaacccgc ctcataattc agcaatgagc
    25561 gattcacaat gccgccttta tgcggcagct ggccgagata gatgttttcc gcgacggtca
    25621 tttccggcac gagatgcagt tcctggtaaa taatcgccac acccgcgtta agtgctgcgg
    25681 tcgtgtcgga aaaggacatt tcctgcccat taatcactac agaacccgtg gttggcgcat
    25741 agttgccgct gaggattttt aagagagttg attttcctgc gccattttca cccatcaacg
    25801 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
    25861 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
    25921 gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt ccggcaaatt
    25981 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcaata
    26041 ccacgtcggt aacttcggta aattttggcg gttcaacgtc ttttgctacc cagttgtaaa
    26101 gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc
    26161 cggttgcctg tgccttagac agttcgctca ccgcatccac accgttaata ccgataccga
    26221 tgatatcggc cgctttaaag ccctgacctt ccgtcgcgcg tacgccgccc agcacggtgc
    26281 tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc agcattgagt
    26341 tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga taaatttgtt
    26401 tttccgggaa tcctgcagct ttcagcgcat ccatagaccc cgtagtacgg cgacgagccg
    26461 tatccagttc gttggcggta atcgccatca ccgcgctttc tttgacatcc cagccccgtt
    26521 tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttggtc gcagccatca
    26581 tcaccagagg aacggtatcc attggcttac ctttggcgtt aacaaactgg tcatccacgg
    26641 caatgacttt catatcgtag ccacgcgcct tcgccacgat ggcagagccg agtttcgggt
    26701 ccggagtaca aataacgaaa ccttttgcgc cactggcggc caggctatcg atcgcgttca
    26761 atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa tccttcccgg
    26821 ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc ttcaccagga
    26881 aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc agaccaatgg
    26941 ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta
    27001 gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac cttttaacta
    27061 aaagataagt gactgtgttg acatagtttt agcgagaaat taattctcca taggagagca
    27121 atatcacatc gcagaattac agtgagaacg tgcataaatt tagcggaaaa agacataagg
    27181 aaaagccaat ttgtcagaca aattgttgaa tgcacagcag attaatccat aagattagcc
    27241 tggaaatcct tgttgtcttt ggtacccatg cgggatgtct tctttttaac cagtcaatag
    27301 gccgcataac ctggcgttga gtttttgaaa tggtgtaata accacaactc aaagatgtgg
    27361 aaaatgcgcg tcattcattt cgccattaat tatcactgtg ctcattaatt aacagaacac
    27421 gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat cacttcatca
    27481 tgaattcaaa ttcattgatt aatatcaaca agatacaaaa agc
//
LOCUS       DAZFFG010000061        27412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFFG010000061 DAZFFG010000000
VERSION     DAZFFG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.061"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            181..2421
                     /gene="fepA"
                     /locus_tag="ACHJ7J_002854"
     CDS             181..2421
                     /gene="fepA"
                     /locus_tag="ACHJ7J_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO5001068.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            2596..3216
                     /gene="entD"
                     /locus_tag="ACHJ7J_002855"
     CDS             2596..3216
                     /gene="entD"
                     /locus_tag="ACHJ7J_002855"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO5001069.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(3338..3490)
                     /gene="hokE"
                     /locus_tag="ACHJ7J_002856"
     CDS             complement(3338..3490)
                     /gene="hokE"
                     /locus_tag="ACHJ7J_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO5001070.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            3459..3727
                     /locus_tag="ACHJ7J_002857"
                     /pseudo
     CDS             3459..3727
                     /locus_tag="ACHJ7J_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3942..5060
                     /locus_tag="ACHJ7J_002858"
     CDS             3942..5060
                     /locus_tag="ACHJ7J_002858"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO5001071.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            5126..5374
                     /locus_tag="ACHJ7J_002859"
     CDS             5126..5374
                     /locus_tag="ACHJ7J_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO5001072.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            5439..5807
                     /locus_tag="ACHJ7J_002860"
     CDS             5439..5807
                     /locus_tag="ACHJ7J_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5001073.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLSDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            5901..6554
                     /gene="nfsB"
                     /locus_tag="ACHJ7J_002861"
     CDS             5901..6554
                     /gene="nfsB"
                     /locus_tag="ACHJ7J_002861"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO5001074.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            6812..8059
                     /gene="ybdG"
                     /locus_tag="ACHJ7J_002862"
     CDS             6812..8059
                     /gene="ybdG"
                     /locus_tag="ACHJ7J_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO5001075.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQKGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(8127..9503)
                     /gene="pheP"
                     /locus_tag="ACHJ7J_002863"
     CDS             complement(8127..9503)
                     /gene="pheP"
                     /locus_tag="ACHJ7J_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO5001076.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(9605..12748)
                     /gene="cusA"
                     /locus_tag="ACHJ7J_002864"
     CDS             complement(9605..12748)
                     /gene="cusA"
                     /locus_tag="ACHJ7J_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO5001077.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(12760..13983)
                     /gene="cusB"
                     /locus_tag="ACHJ7J_002865"
     CDS             complement(12760..13983)
                     /gene="cusB"
                     /locus_tag="ACHJ7J_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO5001078.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDRAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(13999..14331)
                     /gene="cusF"
                     /locus_tag="ACHJ7J_002866"
     CDS             complement(13999..14331)
                     /gene="cusF"
                     /locus_tag="ACHJ7J_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO5001079.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(14355..15737)
                     /gene="cusC"
                     /locus_tag="ACHJ7J_002867"
     CDS             complement(14355..15737)
                     /gene="cusC"
                     /locus_tag="ACHJ7J_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO5001080.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPHAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            15894..16577
                     /gene="cusR"
                     /locus_tag="ACHJ7J_002868"
     CDS             15894..16577
                     /gene="cusR"
                     /locus_tag="ACHJ7J_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO5001081.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            16567..18009
                     /gene="cusS"
                     /locus_tag="ACHJ7J_002869"
     CDS             16567..18009
                     /gene="cusS"
                     /locus_tag="ACHJ7J_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO5001082.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLMD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLVDEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            18159..20396
                     /gene="nrfB"
                     /locus_tag="ACHJ7J_002870"
     CDS             18159..20396
                     /gene="nrfB"
                     /locus_tag="ACHJ7J_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO5001083.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDVLIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            20383..23355
                     /gene="nfrA"
                     /locus_tag="ACHJ7J_002871"
     CDS             20383..23355
                     /gene="nfrA"
                     /locus_tag="ACHJ7J_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO5001084.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEKRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            23356..24246
                     /locus_tag="ACHJ7J_002872"
     CDS             23356..24246
                     /locus_tag="ACHJ7J_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO5001085.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQCFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            24429..25190
                     /gene="envY"
                     /locus_tag="ACHJ7J_002873"
     CDS             24429..25190
                     /gene="envY"
                     /locus_tag="ACHJ7J_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO5001086.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(25236..25312)
                     /locus_tag="ACHJ7J_002874"
     tRNA            complement(25236..25312)
                     /locus_tag="ACHJ7J_002874"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25276..25278),aa:Arg,seq:tct)
     gene            25555..26187
                     /gene="fimZ"
                     /locus_tag="ACHJ7J_002875"
     CDS             25555..26187
                     /gene="fimZ"
                     /locus_tag="ACHJ7J_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO5001087.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(26190..26705)
                     /gene="sfmF"
                     /locus_tag="ACHJ7J_002876"
     CDS             complement(26190..26705)
                     /gene="sfmF"
                     /locus_tag="ACHJ7J_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO5001088.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(26716..>27412)
                     /gene="fimH"
                     /locus_tag="ACHJ7J_002877"
     CDS             complement(26716..>27412)
                     /gene="fimH"
                     /locus_tag="ACHJ7J_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5001089.1"
                     /translation="LGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIF
                     RLKVIRPFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQI
                     VEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQA
                     MVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEG
                     PFSALGYLRVDYQ"
BASE COUNT         6783 a         7241 c         6894 g         6494 t
ORIGIN      
        1 ttgataacta tttgcatttg caatagcgta atggcgcgcc gtgggaagcg cggacattaa
       61 ttaaccaact gcactgcgtg tctttcagga tcaaaggttt tcgcggtagc gggatgcgtc
      121 gtgttgatga cgaccatgcc cgacagttgc aattcgtggc aaaaatgcag gaataaaaca
      181 atgaacaaga agattcattc cctggccttg ttggtcaatc tggggattta tggggtagcg
      241 caggcacaag agccgaccga tactcctgtt tcacatgacg atactattgt cgttaccgcc
      301 gccgagcaaa acttacaggc gcctggcgtt tcgaccatca ccgcagatga aatccgcaaa
      361 aaccccgttg cccgcgatgt gtcggagatc atccgtacca tgccaggcgt taacctgacc
      421 ggtaactcca ccagtggtca gcgtgggaat aaccgacaga ttgatattcg cggtatgggt
      481 ccggaaaaca cgctgatttt gattgacggc aagccggtaa gcagccgtaa ctcggtgcgt
      541 cagggctggc gtggcgagcg cgatacccgt ggtgatacct cctgggtgcc acctgaaatg
      601 attgaacgta ttgaagttct gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg
      661 ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggcg agtggcacgg ctcctgggac
      721 gcatatttca atgcgccaga acataaagag gaaggtgcca ccaaacgcac taactttagc
      781 ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt atggcaacct cgacaaaact
      841 caggctgacg cgtgggatat caaccagggc catcagtccg cgcgtgccgg aacgtatgcc
      901 acgacgttac cagccgggcg cgaaggggtg atcaataaag atattaacgg cgtggtgcgc
      961 tgggacttcg caccattgca atcactcgaa ctggaagcgg gttacagccg ccagggtaac
     1021 ctgtatgcgg gcgataccca gaataccaac tccgattcct atacccgctc gaaatatggc
     1081 gatgaaacca accgcctgta tcgccagaac tactcgctga cctggaacgg tggctgggat
     1141 aacggcgtga ccaccagcaa ctgggtgcag tacgaacaca cccgtaactc gcgtattccg
     1201 gaaggtctgg cgggcggtac cgaagggaaa tttaacgaaa aagcgacaca ggatttcgtc
     1261 gatatcgatc ttgatgacgt gatgctgcat agcgaagtta acctgccgat tgatttcctc
     1321 gttaaccaga cgctgacgct gggtacggag tggaatcagc aacggatgaa ggacttaagt
     1381 tccaacaccc aggcactgac cggaacgaat accggtggcg ctattgatgg cgtgagtgcc
     1441 accgaccgta gcccgtattc aaaagcagaa attttctcgc tgtttgccga aaacaacatg
     1501 gagctgactg acagcaccat cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc
     1561 ggcaataact ggagcccggc gctgaacata tcgcaaggtt taggcgatga cttcacgctc
     1621 aaaatgggca tcgcccgcgc ctataaagcg ccgagcctgt accagactaa cccgaactac
     1681 attctctaca gtaaaggtca gggctgctat gccagcgcgg gcggctgcta tctgcaaggt
     1741 aacgatgacc tgaaagcaga aaccagcatc aacaaagaga ttggtttgga gttcaaacgc
     1801 gacggctggc tggcgggcat cacctggttc cgtaacgatt atcgcaataa gattgaagca
     1861 ggctatgttg ctgtagggca aaacgcagtc ggcaccgatc tctatcagtg ggataacgtg
     1921 ccgaaagcgg tggttgaagg tctggaagga tcgttaaacg taccggttag cgaaacggtg
     1981 atgtggacta ataacatcac ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt
     2041 ttgtcgatca tcccggagta tacgttgaac tcaacgctga gctggcaggc acgagaagat
     2101 ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc agccgaagaa gtacaactat
     2161 aaaggtcagc cagcggttgg accggaaacc aaagaaatta gtccttacag cattgttggg
     2221 ctgagcgcga cgtgggatgt gacgaagaat gtcagtctga ccggcggcgt ggacaatctg
     2281 ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca cgggcgattt ggcaggggcc
     2341 aactatatcg ccggtgccgg ggcgtatacc tataacgagc cgggacgtac gtggtatatg
     2401 agcgtaaaca ctcacttctg atgctaacgt cagattgttg acaaagtgcg cgtcgttcat
     2461 gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat cttgccaatt caatatattg
     2521 caggaccatg taggcctgat aagcgtagcg caccaggcag ttttgcgttt gtcatcagtc
     2581 tcgaatatgg tcgatatgaa aactacgcat acctccctcc cctttgccgg acatacgctg
     2641 cattttgttg agttcgatcc ggcgaatttt tgtgagcagg atttactctg gctgccgcac
     2701 tacgcacaac tgcaacacgc tggacgtaaa cgtaaaacag agcatttagc cggacggatc
     2761 gctgctgttt atgccttgcg ggaatatggc tataaatgtg tgcccgcaat cggcgagcta
     2821 cgccaaccgg tgtggcctgc ggaggtatac ggcagtatta gccactgcgg ggctacggca
     2881 ttggccgtgg tttcccgtca accgattggc gttgatatcg aagaaatttt ttctgcgcaa
     2941 acagcgaccg aattgacaga caacattatt acaccagcag aacacgagcg actcgcagac
     3001 tgcggtttag ccttttctct ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt
     3061 aaggcaagtg agatccaaac tgatgcaggt tttctggact atcagataat tagctggaat
     3121 aaacagcagg tcatcattca tcgtgagaat gagatgtttg ctgtgcactg gcagataaaa
     3181 gaaaaaatag tcataactct gtgccaacac gattaattga caacatctgg tacgattcgc
     3241 ccgcagccat cactgaccgc gggcgaaagt gtaaagcagg tgccttacca tcctgacctg
     3301 acaaccggat atgcggggat tactccccgc acaacggcta cttcttcggt tcgtaagcga
     3361 gaacagccct gaactcaata ttacgttcct tcaccgtaaa ctcacacagc gagtcgccga
     3421 ccagaagcgt aaatcccagc accgtcaaac acagcactat gactgccaca agggcatatt
     3481 tcgtcagcat gtttatattg cctccatgcg aagaagggac taccatccaa attgttcagg
     3541 tttggagtgg cagccccagg gttgatagaa atatctcctg gggcttcatc tttccagacc
     3601 tcagagttaa cctgaaaccg gaaagcttca ggcacccgcc gttatcttac ccttcctcaa
     3661 caaaatggct gtttatttat acagtaaatc ctgtttcgga cttctcactc ctcatttaat
     3721 caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct tcgccaccag
     3781 ccagccgtgc cataacttgt cacattgcaa aagtagccaa attgcttcag gcaccatcag
     3841 ttcaccatca ccaaaaaaaa gaaacattcc agaacttccg catctcgccc agccgctccg
     3901 acttctatac tgaatagaaa acgccaacat aagagaaacc tatgccatta cccgattttc
     3961 atgtttctga accttttacc ctcggtattg aactggaaat gcaggtggtt aatccgccgg
     4021 gctatgactt aagccaggac tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg
     4081 ccggagaggt aaagcacgat atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc
     4141 gtgatatcaa ccaggctgcc gggcaatttt cagcgatgca gaaagtcgta ttgcaggcag
     4201 ccgcagacca tcatctggaa atttgcggcg gtggcacgca cccgtttcag aaatggcagc
     4261 gtcaggaggt atgcgacaac gaacgctatc aacgaacgct ggaaaacttt ggctatctca
     4321 tccagcaggc gaccgttttt ggtcagcatg tccatgttgg ctgtgccagt ggcgatgacg
     4381 ccatttattt gctacacggc ttgtcacggt ttgtgccgca ctttatcgcc ctttccgccg
     4441 cgtcgccgta tatgcaggga acggatacgc gttttgcctc ctcacgacca aatatttttt
     4501 ccgcctttcc cgataatggc ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc
     4561 tgtttcgctg cctgagttac accacgatga tcgacagcat taaagatctg cactgggata
     4621 ttcgccccag tcctcatttt ggcacggtgg aggttcgggt gatggatacc ccgttaaccc
     4681 ttagccacgc ggtaaatatg gcgggattaa ttcaggctac cgcccactgg ttactgacag
     4741 aacgcccgtt caaacataag gagaaagatt acctgctgta taaattcaac cgtttccagg
     4801 cctgtcgcta tgggctggaa ggcgtcatta ccgatccgca cactggcgat cgtcgaccgc
     4861 taacggaaga taccttgcga ttgctggaaa aaatcgcccc ttccgcacat aaaattggtg
     4921 catcgagcgc gattgaggcc ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc
     4981 tgatgcgcga tttcgtcgcc gatggcggct cgctgattgg gctggtgaaa aagcattgtg
     5041 agatctgggc cggtgactaa gcctggaatt gccttacaga agaatatccc tgacaatggt
     5101 gttttaactt acatttaaca atgttatgaa acacccttta gaaaccttga ccaccgcagc
     5161 aggcattttg ctgatggctt tcctctcttg cctgctgctg cccgcccccg cactggggct
     5221 gacgctggca caaaaactgg tgaccacgtt ccatctgatg gatcttagtc agctttacac
     5281 tttattgttt tgtctgtggt ttttagtgct gggcgctatt gagtattttg ttctgcgctt
     5341 tatctggcga cgctggttct cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg
     5401 catactttgc ttattctcct tcgcctaaag gaacgtttat ggataagcaa tcactgcacg
     5461 aaacggcgaa acgcctggcc cttgagttac cctttgtcga gctttgctgg ccttttggcc
     5521 cggagttcga tgtttttaaa attggcggca agatttttat gctgtcgtcg gagctacgcg
     5581 gcgtcccctt tatcaatctg aagtccgatc cacaaaaatc cctgttaaat cagcaaatat
     5641 acccaagcat taagccaggg tatcacatga ataaaaagca ctggatttcg gtgtatcccg
     5701 gcgaggaaat ctccgaagcg ttacttagcg atctgatcaa cgattcgtgg aatctggtgg
     5761 ttgatggtct ggctaaacgc gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat
     5821 agcgcatttt tctcgcttac catttctcgt tgaaccttgt aatctgctgg cacgcaaaat
     5881 tactttcaca tggagtcttt atggatatca tttctgtcgc cttaaagcgt cattccacta
     5941 aggcatttga tgccagcaaa aaacttaccc cggaacaggc cgagcagatc aaaactctcc
     6001 tgcaatacag cccatccagc accaactccc agccgtggca ttttattgtt gccagcacgg
     6061 aagaaggtaa agcgcgtgtt gccaaatccg ctgccggtaa ttatgtgttc aacgaacgta
     6121 aaatacttga tgcctcgcac gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct
     6181 ggctgaagct ggttgttgac caggaagatg ctgatggccg ctttgccacg ccggaagcga
     6241 aagccgcgaa cgataaaggt cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg
     6301 atgatgcaga gtggatggca aaacaggttt atctcaacgt cggtaacttc ctgctcggcg
     6361 tggcggctct gggtctggac gcggtaccca tcgaaggttt tgacgccgcc atcctcgatg
     6421 cagaatttgg tctgaaagag aaaggctaca ccagtctggt ggttgttccg gtaggtcatc
     6481 acagcgttga agattttaac gctacgctgc cgaaatctcg tctgccgcaa aacatcacct
     6541 taaccgaagt gtaattctcc tgacagccgg gaccccatcc cggctcagat tgctgacaaa
     6601 gtgcgctttg tttatgccgg atgcggcgtg accgccttat cctgcctaca agttcgtgcc
     6661 aattcattca ggtaggcatg ataagcgtag cgcatcaggc aattttgtat ttgccatcag
     6721 ccctcgcagg aaatcccccc tcagattctc ctgatttgca taaccctgtt tcagccgtca
     6781 tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc aggttgaaga
     6841 tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg gtattatttt
     6901 tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga ccttcgaaaa
     6961 acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata aactcttcca
     7021 ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat tctggctgca
     7081 aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga tcatgatgta
     7141 tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc agaaattccc
     7201 ggcagcatcc cagttaccgc tgaaagggat atttcagggg attaaactga tcggcgcgat
     7261 tctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga ttctgatcag
     7321 cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga ttcttggtct
     7381 ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgatt ggctggagat
     7441 gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg tcaaagtgcg
     7501 taactgggac aataccatta ccactattcc cacctggtct ctggtttctg actcctttaa
     7561 aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta tcagtattga
     7621 tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata aagcgcattt
     7681 gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc gccagaaagg
     7741 ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct ttcgtgccta
     7801 tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgactt taatggtacg
     7861 ccaactggct ccaggtgata acggtttacc gctcgagatc tatgcgttta ccaacaccgt
     7921 ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat ttgcgattgt
     7981 cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc gctctctggc
     8041 gggtgcattt aagcagtaat taaaaaaaac gctctcatcg aatggatgag agcggacatc
     8101 gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa tgctacaaac
     8161 aggaatacaa tccacaccgg cagcagaatc gctgacaagc gcatatcatc catcgtgcac
     8221 atcagcagca aaatcatgcc gaggaaggcg atgcaaagat agttgccgaa cggatagagc
     8281 agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ctgcacgaaa acgcagatgc
     8341 gccagacaga tcataatcca gttcaacagc agcgttgcta ccaccagcgc catcagcaga
     8401 ccaaacgctt tttgcggcag cagatagttg attaacacca ccagcgaagt gatcgctccg
     8461 gaaagcatca gcgagttaat cggcacgccg cgacggctaa cacgggtaag gaacttcggc
     8521 gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac cccgctgtta
     8581 tacactgaca gcgatgctac cagaatgacg aagttcagcg cagaagctac cacgttgctg
     8641 tcgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc cacccacgga
     8701 tagagcgcca gtaaaaccac cagtgaaccg atgtaaaaca gcaggatgcg atacaccacc
     8761 tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc agtaatccca
     8821 atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat cagcccattc
     8881 cagccggtgg cgaagaaacc accgtagcgc cagaggttgt cgatactggc tttctcgccg
     8941 ccgtgaccag aaaacagcag ccacaggcca aagccgatca taccgatgat tgccagcact
     9001 ttaatcagcg caaaccagaa ctcggtttcg ccatataagc gcacgttcac caggttaacg
     9061 gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc cgggaaccag
     9121 tactgcatat agatgcccgc agcggtcagc tctgccattc ccaccagcac gaacattacc
     9181 cagtagttcc agccagagag gaagcccgca aacggtcccc agtatttata ggcaaagtgg
     9241 gcaaatgaac cggataccgg ctcctcaacc accatttcgc caagctggcg cataatcagg
     9301 aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc catctgaatc
     9361 gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccagcgc aatcagttga
     9421 atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc agtatcttcc
     9481 gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt gaggggcacc
     9541 ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac ggggtatcct
     9601 gcttttattt ccgtacccga tgtcggtgca gccacatcag cttatacgcc gccgggataa
     9661 taaacagcga cagcaaaggc gcggtgatca tgccgccaat catcggcgcg gcaatccggc
     9721 tcatcacctc tgaccctgct cctgttcccc acagaatcgg cagcagacca gcgataatca
     9781 ccgccaccgt catcgctttc gggcgcacgc gcagtaccgc gccgtgatac aacgcctcat
     9841 ccagcttctg ctcactgaat gtttgcggat tattcaacga cggctcggcc tctatggcgt
     9901 gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca agggcaataa
     9961 aaccggtgcc cgtcgccaca gaaagatgaa agcccatcca ccacagcaac cagatgccgc
    10021 ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc cgacggaacg
    10081 ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt ttcagcttat
    10141 ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg cccggtttta
    10201 actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc atgtcacgat
    10261 cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc agcatcgacg
    10321 gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc ttcatcggcg
    10381 tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc ggataacgca
    10441 gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc ccgcccaccg
    10501 cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg gctttttcac
    10561 ggttaatctc aacgttgata tagcgcccac cttccagacg ctcggcaagc gcagaagcta
    10621 cgcctggcac cgttcgcgcc acttcttcaa tttgctccgc catcgcgtcg atatccgcca
    10681 gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag agcatatcga
    10741 tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc acggtgttat
    10801 ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc tgcggcttaa
    10861 gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt tccgctttcc
    10921 cggttttgcc aaatacccgc gccacttcag gtacgctcat aatcagcttg tcggtttttt
    10981 gcagcatact cgccgcctct gctgcggaga tccccggcag cgtcgatggc atatataaca
    11041 agtcgccttc attgatctgc ggtaaaaact ccccgccaac tttattgagc ggccagagaa
    11101 ccgtcagcac cgaaagcgct gccaccagca gcgtggtttt cggccagtgc agtactttca
    11161 gcaatagcgg atgataaaca cgaatcaaaa agcgattgag cgggttactg ctttccggcg
    11221 gaattttgcc acggatccag taacccatca ggatagggat caccacgatc gccagcagcg
    11281 ccgcgcccgc catcgcatac gttttggtga atgccagcgg gccaaacaga cgcccttcct
    11341 gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga ctgataaaca
    11401 gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt ttattatcca
    11461 gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgtgca ttctcgatca
    11521 tgacgatagc agcatcgacc atcgccccga cggcaatcgc aatgccaccc agcgacataa
    11581 tattggcatt cagtccctgg aagtgcatga caataaaagc aatacacaac cccagcggca
    11641 acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgca cagactaccg
    11701 ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcg cggtcaatga
    11761 gctggctgcg atcgtatgtt gtaactatct ccacgccttc cggcagacta cttttcagcg
    11821 tttccagttt gtccttcacg gcggcgatta cttctcgggc gtttttgccg gagcgcagga
    11881 tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc cggcgcatct
    11941 ccgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca ttttcactgg
    12001 cttttaaaac gatgtgatta aagtcgtcga gcgtttgcag atagccgctg gcgcgcacca
    12061 tatattccgc ttccgccagt tcaatcgacg aaccgcccgc ttcctggttt gaagcatcca
    12121 gcgcgctttt tacttcggcg agactgatac catattgcgc caggcgctgg ggatcgataa
    12181 ccacctgata ctctttcacc acgccgccca ccgacgccac ttccgcaacg tcagggatgg
    12241 tttttagctc atatttgaga aaccagtcct gcaatgagcg taaatcggcc agatcgtgct
    12301 taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg gcatctggtc
    12361 ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg tactccagca
    12421 cccgcgagcg cgcccagtac ggatcggtgc catcttcgaa aatgacatac acataagagt
    12481 cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac atggtggtag
    12541 ttagcggata agtcacctga ttttcaacga tttgcggtgc ctgaccggga tagctggttt
    12601 taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgtg ttaatgatgg
    12661 tccaggtgcc ccagatactc agaaataacg cgcccatcag caccagaaaa cggttcgcca
    12721 ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat gggtagcact
    12781 ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa tcaggaacag
    12841 gccgctggaa accacctttt caccttccgc cagaccagag cgtaatgcgg tgacaccttg
    12901 cgatgcctgg aaaacggcaa cgcgttttgg tacaaagcgc ccgtcggcat caacggtaat
    12961 cacccgctgt tcgctgccgg tatcaatcag cgcttgtgac ggaatgagca gcatcggttc
    13021 gctggcggta ttgagttgca accaggcgtt cattcccggt tttagcgcct cgtcggcgtt
    13081 gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag gtagtagcgt
    13141 ccatttgcgg atggtgagtg ttttatcagg tcgcgccgga acggtgagcg taaactgcga
    13201 ggcatctttc accagccagg cgatagactc cgggatcgca gcagtgaccc acaccgggtc
    13261 cataccctga attttcgcga ccacgttatc tttggcgata ttcattcccg cgcgcagatc
    13321 aaacgcggtg atcacgccat caatgggtgc tttgagcgta aagcgagtct ggattttttg
    13381 cgtggcgatc aggcggcgaa tatccgcctc cggcattccc gccagtcgca gccgctcaag
    13441 aatgccttca gtctgggtcg ccgtaccgcc ggtttcgcgc agcagtaaat actcactctg
    13501 cgcttccacc cagtcgggaa tggtcaggtc gagaagcggt gtgccctttt gcactttatc
    13561 gcccacggta agcggataca ccttgtcgat aaacccggca gcgcgagcct gcacaatggc
    13621 atactgatac tcgttgtaac tgacattcgc cgggaaactc tgggcaaaag tcagcggccc
    13681 gcgcgtgacg gtagccgttt tcacccccag attctgcgtc tgagtcgggt caatgcgcac
    13741 accagacgca gaactctctt catcggcata tttcggcacc agatccatat ccataaacgg
    13801 cgatttacct ggtttatcga accgcgtatt gggatacatc gggtcgtacc agaataagat
    13861 tttacgttct gcggtcgacg ttttttctgc gggcggttca gcccggtcaa accaggtaaa
    13921 acctgccgca gaaataatac cgcccgcgat catgctgccg ataataagcg cgattttttt
    13981 catctcatta aacctgggtt actggctgac tttaatatcc tgtaataaag aaaggttgcc
    14041 ctgctggaca aaattaaacg ccactttgtc gccagtttta atttcactca ttttcgtctg
    14101 cggagtgatg gtaaagcgca tggtcatctc cggccagttc acggcagcaa tcggatcgtg
    14161 atggatggtg atttttttgc tttccagatc aatacccttt accacgccgg tggcactaat
    14221 gacctgtggt tgtgcttcgc tcatggtttc atgatgatgt tcgttagcct gggcattaaa
    14281 gccaataacg gtaaacagac tgaacattgc gacttgcagt gcttttttca tttattctct
    14341 cctggagtta aaaattattg ctgccaaccg ccaccaagtg cggtatataa agaaatttcg
    14401 ttgacctgac gggcataatt caggtcaagt aaggtttgtc gggttgcaaa taacgaacgc
    14461 tcggcatcca gcacttccag ataacttacc gcgccgtgct gatataatgc ccgcgcccgt
    14521 tgcaaagtaa tttgcagcga cgccagataa cgctgctggg cgctgatttg atcgttcagg
    14581 ctttgacgta atgccagcgc atctgccact tctttaaagg cgttctggat tttctgttca
    14641 taattcacta ccgactgctg ctggcgaatt tcggcgatat ccagattggc ctggttgcgt
    14701 ccggcattaa aaatggggat ctcaatttta ggaataaaat tccacatccc gctgctggcg
    14761 ttaaataatg acgacagatc gctgctggcg gttgatattc cgctggtcag gcttatagat
    14821 gggaaaaatg ccgcgcgtgc ggccccaata ttggcattag ccgccattaa cgcgtgttca
    14881 gcttccataa tatccgggcg ctgcaataag atttgcgacg acaagcccgc cggtaattta
    14941 acgctttgca ggctgtcgct gtttactgtc tgcgcatgcg gcagcttgcc gtagcttccc
    15001 agtaacagtt gcaatgcatt attcgcctgc gccagttccc cctgacgttt agcgatgtcg
    15061 ctgcgggtac tttcaatcac cccgcgagcc tgttccagcg ccagaacatt actgctacct
    15121 gtcaacagtt gtttttcgac aaacgcatat gactgctgat aattacgcag cgtttcttcg
    15181 gctatttgca attgcgcata cgccagctgc tgattgaaat agctttgcgc gacattagaa
    15241 accagcagaa tatgcaccgc gcgctgagct tcctcagtgg ctaaataatt ttgtcgctcg
    15301 gcttcgctca tgttctttaa gcgcccgaaa aaatcgaggt caaagctggc gttaaggcca
    15361 gtagaaaact cccgcgtcgt ggctgaatcg cctttaagat tgccgctcca gctgccgctg
    15421 ccctcaccat tgagctgtgg gtagcggtcg gcatcagtca gacgatattg cgcccgtgcc
    15481 tcctgcactt tcagcgtcgc catgcgcaaa tcccggttat tctccagcgc ctcgctaatc
    15541 agcgtcttca cctgattatc aacaaaaaag gtgcgccagc ccgcgttctg atagttatct
    15601 gctgcgttaa ccaggccgtt ctggctgagt gagaactgct gaggaacggg cattgccgga
    15661 cgctgataat ccggtgccag tgaacaaccg gttagcgcaa gggccacaca aaatggcaga
    15721 agtttacaag gagacatagg ctcataattt ctggtgattt tataccgcca actttactcg
    15781 ccaggctctg attttccggt gacaggaaaa tgacaaaatt gtcattttgc caataagcga
    15841 ttgccatctg atcccgctac tctagaattg cccgggcaac atgcggagga aatatgaaac
    15901 tgttgattgt cgaagatgaa aagaaaaccg gagaatactt gaccaaaggg ttaaccgaag
    15961 ccggttttgt ggtcgatttg gccgacaacg ggctgaatgg ctaccatctg gcgatgaccg
    16021 gtgattatga tctgataatc ctcgatatta tgctgccgga cgtgaacggc tgggatatcg
    16081 tgcgcatgtt acgctccgcc aataaaggga tgccgattct gttgcttacc gcgcttggca
    16141 ccattgaaca tcgcgtcaag gggctggagt tgggggcaga tgactaccta gtgaagccat
    16201 tcgcttttgc tgaactgctg gcgcgggtgc gcactctcct gcggcgcggg gcggcggtga
    16261 ttatcgaaag tcagtttcag gttgccgatc tgatggtcga tctcgtcagc cgcaaagtca
    16321 cccgcagcgg cacgcgcatc actttgacca gtaaagagtt tactctgctg gagttttttc
    16381 tccgccatca gggcgaagtg ctgccccgct cgcttatcgc ctcgcaggta tgggacatga
    16441 attttgacag cgacactaac gccattgatg tggcggtgaa gcggctacgc ggcaaaatcg
    16501 acaacgactt tgagccgaag ctgattcaaa ccgtgcgcgg cgtgggttac atgcttgagg
    16561 tgccggatgg tcagtaagcc atttcagcgc ccgttttcgc tggcaacccg cctgaccttt
    16621 tttatcagcc tggccaccat cgcggcgttt ttcgcctttg catggatcat gatccactca
    16681 gtaaaagtgc attttgccga gcaggatatt aatgatttaa aagagattag tgccaccctt
    16741 gaacgcgtac taaatcaccc tgacgaaacg caagcccgac gcttaatgac gctggaagat
    16801 atcgtcagtg gttattccaa tgtgttgatt tccctggcag atagtcaggg taaaacggtg
    16861 tatcactccc ccggtgcgcc ggatatccgc gagtttgcgc gcgatgccat acccgataaa
    16921 gacgcgcggg gcggcgaggt gtttctcctc tccggcccga cgatgatgat gccaggccac
    16981 ggtcacgggc atatggaaca cagcaactgg cggatgatta acttaccggt tggcccgctg
    17041 atggacggca aaccgattta tacgctctac atcgcgcttt ctatcgattt tcatcttcat
    17101 tacataaatg atttgatgaa taaacttatt atgaccgcat cggtaatcag catcctgatc
    17161 gtctttatcg tgctgttggc ggtacataaa ggtcacgcgc cgatccgcag cgtcagccgt
    17221 caaatccaga atatcacctc gaaagatctc gacgttcgcc tcgacccgca gaccgtgccc
    17281 attgagctgg aacagctggt actgtcgttc aaccatatga tcgagcgtat tgaggatgtc
    17341 tttacccgcc agtccaattt ctcagcggac atcgcccacg aaattcgcac accgattacc
    17401 aatctcatca cgcaaaccga aatcgccctc agccagtcgc gcagccagaa ggagctggaa
    17461 gatgtgctct actctaatct cgaagagctg acgcggatgg cgaaaatggt cagcgatatg
    17521 ctgtttctcg ctcaggccga taacaaccag ttaatccccg aaaagaaaat gctcaacctg
    17581 gtggatgaag tcggcaaagt gttcgatttt ttcgaggcgt tagcggaaga tcgcggcgtg
    17641 gagttgcgat ttgttggcga cgaatgtcag gttgcgggcg atccgctgat gctgcgtcgg
    17701 gcgttaagca acctgctttc taatgccctg cgttatacgc cacccggaga ggctattgta
    17761 gtgcgctgcc agacggtcga tcaccaggtg caagttatcg tcgaaaaccc cggtacgccc
    17821 attgcgcccg agcacttacc gcgattgttt gaccgtttct accgcgttga cccctcccgc
    17881 caacgaaaag gcgaaggtag cggcattggg ctggcgatag tgaaatcaat tgttgtcgcg
    17941 cataaaggca cggttgccgt aacgtcagat gcgcggggga caaggtttgt tatcacatta
    18001 cccgcttaat tattcagcaa acggcaactt ttataaccag tgtaaaaata acgtgccgca
    18061 atagtcatac ctattaatgg taaaaagctg tcacaattca taaaaaacct taatatacgc
    18121 caccctaaac ataaccagcg ttaatgtaag gtttttgtgt ggactggctt cttgatgttt
    18181 ttgctacctg gctctacggc ttaaaagtaa tcgcgataac gttagcggtc atcatgttca
    18241 tcagcgggct ggacgatttt tttattgatg tcgtctactg ggtacgccgc attaaacgca
    18301 agttgagtgt ttatcgccgc tacccgcgaa tgagttaccg cgaactgtat aaaccagatg
    18361 aaaaaccgtt agcgattatg gttccggcgt ggaatgaaac gggcgtcatc ggcaatatgg
    18421 ccgagctggc ggcgaccacg ctcgactacg aaaactatca tatctttgtt ggcacctacc
    18481 ccaacgaccc ggacactcag cgtgatgttg acgaagtgtg cgctcgcttc ccgaatgtgc
    18541 ataaggtagt ctgcgcgcgt cctggcccca ccagcaaagc cgactgtctg aacaacgtgc
    18601 tggacgccat cacccaattt gagcgtagcg ccaatttcgc ttttgctggt tttattctgc
    18661 atgacgccga agatgtgatt tcaccgatgg aattgcgtct gttcaactat ctggtcgagc
    18721 gtaaagatct gattcagatc ccggtgtatc cgtttgaacg cgaatggacg cacttcacca
    18781 gcatgactta cattgatgag ttttcagagc tgcatggcaa agatgttccg gtgcgtgaag
    18841 ccctcgccgg acaagtgccc agcgcaggcg tcggcacctg tttcagccgc cgcgccgtga
    18901 ccgcactgtt agctgacggt gacggtattg ctttcgacgt gcagagtctt actgaagatt
    18961 acgacattgg cttccgcctg aaagaaaaag gtatgacgga aatttttgtc cgttttccgg
    19021 tggtggacga agccaaagaa cgcgagcagc gtaaattttt acagcacgca cggacgtcaa
    19081 acatgatctg cgtgcgcgaa tatttccccg ataccttttc gactgcggtt cgacaaaaat
    19141 cccgctggat catcggcatt gttttccagg gctttaaaac ccataaatgg acctccagcc
    19201 tgacgctgaa ctactttctc tggcgcgacc gcaaaggggc aatcagtaac tttgtcagct
    19261 tcctcgcgat gctggtgatg atccagcttt tgctgttgct ggcgtatgaa agtttgtggc
    19321 ccgatgcctg gcatttcctt tctattttca gcggcagcgc atggttaatg accctgctgt
    19381 ggctaaactt tggtttgatg gttaaccgca tcgtgcagcg ggtgattttc gttactggct
    19441 actacggcct gacgcagggg ctgctttccg tcctgcgtct tttctggggc aacctgatta
    19501 acttcatggc caactggcgc gcgttaaaac aggtacttca acacggcgat ccacgtcgcg
    19561 tggcgtggga taaaacaacg catgacttcc ccagcgtcac tggcgatacc cgctcgttgc
    19621 gcccgttagg tcaaattctg ctggaaaatc aggtcatcac tgaagaacaa ctcgatacag
    19681 cactgcgtaa tcgcgtcgaa ggtctacgcc tgggcggttc aatgctgatg caggggctga
    19741 ttagcgccga gcaactggca caggcgttgg cagagcaaaa cggcgtggcg tgggaatcca
    19801 tcgatgcctg gcagatcccg tcctcgctga ttgccgaaat gccagcctcc gtggcgctgc
    19861 attatgcagt gctgccgctg cgtctggaaa atgatgtgtt aattgtcggc agtgaagatg
    19921 gtattgaccc ggtttcgctg gcggccctga cgcgtaaagt cggacgcaaa gtgcgttacg
    19981 tcattgttct gcggggacaa attgtcacgg ggttacgtca ctggtatgca cgccgacgcg
    20041 gtcacgatcc gcgggcaatg ttgtacaatg cggttcagca tcagtggctc acggaacagc
    20101 aggccggtga aatctggcgg caatatgtgc cgcatcagtt cctgttcgcc gaaatactga
    20161 ccacgctcgg tcatattaat cgttcagcaa ttaacgtgtt gttattgcgc catgaacgca
    20221 gttctctgcc gctcggcaag tttttggtca ccgaaggcgt tatcagccag gaaacgttgg
    20281 atcgcgtcct gacaattcaa cgcgaattac aagtttcgat gcaatcacta ttactcaaag
    20341 caggtttaaa cacagaacag gttgcgcaac tggagtccga aaatgaagga gaataacctt
    20401 aatcgcgtca tcggatggtc tggtttactg ctgacgtctt tattgagtac cagcgcactc
    20461 gcagacaata tcggcaccag cgcagaagag ctggggctga gcgattatcg ccattttgtt
    20521 atttatcccc gtctcgataa ggcgctgaag gcacagaaaa ataacgacga agcaaccgcc
    20581 atccgcgaat ttgaatatat acaccagcag gtgccggata atattccgct gactttatac
    20641 cttgcggaag cctatcgcca ttttggtcat gatgaccggg cgcggctgtt gcttgaggat
    20701 caactgaaac gtcacccagg agatgcccga cttgagcgca gtctggcggc tattccagtt
    20761 gaagtgaaaa gcgttacgac agttgaagaa ctgcttgccc agcaaaaagc gtgcgatgct
    20821 gcgccgaccc tgcgttgtcg cagtgaagtc gggcagaatg ccctacgtct ggcacaatta
    20881 cctgttgcca gagcgcaact gaacgatgcg acgtttgctg catcgccgga aggaaaaacg
    20941 ttgcgaaccg atctgctgca acgggcaatc tacctgaaac aatggtccca ggcagatacg
    21001 ctatacaatg aagcacgcca gcagaacaca ttaagcgcgg cagaacgccg tcagtggttt
    21061 gacgtgcttc ttgccggaca gctggacgat cggatcctgg cgctgcaatc acaggggatc
    21121 ttcaccgatc cgcagtcata tattacttac gcgaccgcgc tggcttatcg tggcgaaaaa
    21181 gcacgcctcc agcattatct cattgaaaat aagccgctgt ttaccacaga cgcacaagag
    21241 aaaagttggc tctatctgtt atctaaatac agcgccaacc ccgttcaggc attggcgaat
    21301 tatacggtgc agtttgccga taaccgccag tatgttgttg gcgcgacgct accggtgctg
    21361 ttaaaagaag gtcagtacga cgcagcgcaa aaactgctcg ccaccctccc cgccaatgaa
    21421 atgcttgagg agcgttatgc tgtcagcgtg gcgacccgta acaaggctga agctttgcgt
    21481 ctggcacgat tgctgtatca gcaagaaccg gcaaatctta cccgcctgga tcaactgacc
    21541 tggcaactga tgcagaacga gcagtcacgc gaagctgccg atttattgct gcaacgctat
    21601 cctttccagg gcgatgcgcg tgtcagccag actttaatgg cgcgactggc gtctcttctg
    21661 gaaagtcatc cttacctggc aactccagcg aaggtggcga ttttatcgaa acccttaccg
    21721 ctggcggagc aacgtcagtg gcaaagtcag ttgccgggta ttgcagataa ttgcccggca
    21781 atagttcgct tgctgggcga tatgtcgcct tcctacgatg ctgccgcctg gaaccgtctg
    21841 gcaaagtgtt atcgggacac gctacccggt gtggcgttgt atgcatggct tcaggccgaa
    21901 caacgacaac cgaacgcctg gcaacatcgt gcggtagcct atcaggcgta tcaggttgag
    21961 gactacgcca ccgcactggc ggcctggcag aaaatcagtc ttcacgacat gagcaatgag
    22021 gatctgcttg ctgctgccaa taccgcccag gcggcaggaa atggtgcggc tcgcgatcgc
    22081 tggctacaac aggcagaaaa acgtggactg ggaagcaatg cgctctactg gtggctgcat
    22141 gcgcaacgtt acattcctgg tcagccggaa ctcgcactga acgatctcac gcgctcaatc
    22201 aatattgcgc cttctgccaa cgcttacgtt gcgcgggcga caatttatcg ccaacgtcat
    22261 aatgtcccgg ccgcggtgag tgatttgcgc gccgcgctgg aactggaacc gaataatagc
    22321 aacacccagg cagcgcttgg ttacgccttg tgggatagcg gtgatatcgc acagtcgcgg
    22381 gaaatgctcg aacaggcgca taaagggcta ccggacgatc cggcactgat ccgacaactg
    22441 gcctatgtga accagcgtct ggatgacatg cctgcgacgc agcactacgc ccggctggtg
    22501 attgatgaca ttgataatca ggcgctgata accccactga ccccagagca aaatcagcaa
    22561 cgcttcaatt tccgccgtct gcatgaggag gtcggtcgcc gctggacgtt cagtttcgat
    22621 tcatctatcg gcttgcgttc cggcgcaatg agtaccgcta acaataatgt cggcggcgca
    22681 gcgccaggga aaagctatcg tagctatggg caactggaag ccgagtaccg catcggacgc
    22741 aatatgctgc tggaaggcga cctgctctcg gtttacagcc gcgtctttgc cgataccgga
    22801 gaaaacgggg tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct gcgctggaag
    22861 ccgctgcgcg atcagatctt tttcctcgcc gtcgaacagc agttgccgct gaacgggcaa
    22921 aatggcgcat ccgataccat gctgcgcgcc agcgcctcat tctttaatgg cggcaaatac
    22981 agcgacgaat ggcacccgaa cggttcaggc tggtttgccc aaaacctgta cctcgatgcg
    23041 gcgcaatata tccgccagga tattcaggcg tggacggcag attatcgcgt cagctggcat
    23101 cagaaggtag ctaacggaca gactattgag ccttacgctc acgttcagga caacggctat
    23161 cgtgataaag gcactcaggg cgcgcagctt ggcggtgtcg gggtccgctg gaatatctgg
    23221 accggcgaga cgcactacga cgcctggccg cacaaagtca gtctcggcgt cgagtatcaa
    23281 cataccttta aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt tctcaccatt
    23341 ggagtgcact ggtaaatgcg taagttcatt ttcgtattgc tgacactgct tttggtcagc
    23401 cctttttcct ttgcgatgaa aggtattatc tggcaaccac aaaaccgtga tagtcaggtt
    23461 accgataccc agtggcaggg gctgatgagt cagttacgtt tgcaatgctt cgataccctg
    23521 gttttgcaat ggacccgtta cggcgatgca ttcacccagc cagaacagcg cgcgttattg
    23581 tttaagcggg ccgcagctgc gcaacaggct ggcctgaagc tgattgtcgg gctgaacgcc
    23641 gatccggaat tttttatgca ccagaaacag tcatccgcgg cgctggaaag ctatcttaat
    23701 cgcctgctgg ctgccgatct ccagcaagcc agattatgga gtgccgtacc tggcgtaacg
    23761 ccggatggct ggtacatcag cgcggaaatt gacgacctga actggcgcag cgaagccgcc
    23821 cgtcagcctt tgctaacatg gttaaacaac gcgcagcggc tgattagcga tgtttcagca
    23881 aaaccggttt atatcagtag ttttttcgcc ggaaacatgt cgccagatgg ctatcgccaa
    23941 ctgctggaac acgttaaagc aaccggcgtt aatgtctggg tacaggatgg cagcggcgtg
    24001 gataaactta ccgctgaaca gcgtgaacgt tatctacagg ccagcgccga ttgccaaagc
    24061 tccgccccag caagcggcat tgtttatgaa ctgtttgtcg ccggcaaagg caaaaccttt
    24121 acagcgaaac cgaaaccgga cgcagaaatt gcctcgctgt tagcgaaacg ttcctcttgc
    24181 ggtaaagaca ctctctattt ctctctgcgc tatttgcccg tcgcgcacgg cattctcgag
    24241 tattaaatct cctccaggta agtcgggtac gacctggctt acctctttcg ctctcgtcag
    24301 aattccatca agattaattg ctaaaaagcg tgctaaagaa agaagatcgt tatcggcgaa
    24361 cgggccgatc actcatgcag gaagcataga tcctttgagc ggagtttctg atatctgagg
    24421 agtgcgaaat gcaattgagc agcagtgaac cttgcgtggt gatcctgacc gaaaaagagg
    24481 tagaggtaag cgtcaataac catgctacgt ttacccttcc gaaaaactac ctggccgcct
    24541 tcgcgtgcaa caataacgtc attgaactct caacgttaaa tcacgtatta atcacccaca
    24601 tcaaccgtaa catcatcaac gattatctgt tgtttttaaa taagaactta acctgtgtaa
    24661 agccctggtc gcggctggct accccggtta tcgcttgtca tagccgtaca ccggaagtgt
    24721 tccggctagc cgccaaccac agcaagcagc aacccagcaa accctgcgaa gcggagttga
    24781 cgcgcgcatt gctttttacc gtattgtcta actttcttga gcaatcgcgg tttattgccc
    24841 tactgatgta tatcttacgc agcagcgtcc gcgacagcgt ctgccgcatt attcaaagcg
    24901 atattcagca ttactggaat ctgcgaattg tcgccagttc gctatgttta agccccagcc
    24961 tgctcaaaaa gaaattaaaa aacgaaaata ccagctatag ccagattgtc acagagtgtc
    25021 gtatgcgtta cgccgtacag atgttattga tggataacaa aaatatcact caggtggcgc
    25081 aattatgtgg ctatagcagc acgtcgtact ttatctctgt ttttaaggcg ttttacggcc
    25141 tgacaccgtt gaattatctc gccaaacagc ggcaaaaagt gatgtggtga agggcaaacc
    25201 ggaaacggat aagacgggca taaatgaaga agaaatggcg cgccctgcag gattcgaacc
    25261 tgcggcccac gacttagaag gtcgttgctc tatccaactg agctaagggc gcgttgatac
    25321 cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct tgctgagtca atggcttttg
    25381 atctggttgc tgaacaagtg aacgaccgcg tctgattttc tgatttattt cgctatagcg
    25441 gcaaacaaat gcacaccgct gcgcgtctga atcaagaaaa cccgtatttt catgtatcaa
    25501 agtacaattt accgccctaa cggaaaattg tccgctccta tgagactggt aactatgaaa
    25561 ccaacgtcgg tgatcattat ggatactcat cctatcatca gaatgtctat tgaagttctg
    25621 ttgcaaaaaa acagtgaatt gcagattgtc ctgaaaacgg atgattaccg cataaccatc
    25681 gattatctcc gaacccgtcc tgttgattta atcattatgg atatagactt gcccggaaca
    25741 gacggtttta ccttcctgaa aaggatcaaa caaatccaga gcacagtgaa agtgttattt
    25801 ttatcatcga aatcagaatg cttttatgct ggcagagcga tacaagctgg tgctaacggt
    25861 tttgtcagta aatgcaatga tcagaatgat atttttcatg ccgttcagat gatcctctcc
    25921 ggatacactt tttttcccag cgaaacgctc aactatataa aaagcaataa atgtagtacg
    25981 aatagttcaa cgatcactgt gctatctaat cgtgaagtga cgatattacg ttatctggtt
    26041 agcggattat ctaataaaga aattgccgat aagttattac ttagcaataa aacagttagt
    26101 gcgcataaat ctaatattta tggcaagcta ggtttgcatt caattgtaga gcttatcgac
    26161 tacgccaaat tatacgaatt aatataatat taattataat tgatcataaa tatcgcatcc
    26221 gctttcgcca cacctggccg aacaccgctg gctaacgctc gataattggc aaaaaagttt
    26281 agtgtgacat tgccatttgc atctacttcc tcagtcgggc tcgcctcccc cagtgcgagc
    26341 cgggagcgat cgctattacg taattcgatg gcgacggttt gtgccattgc gggatcatcc
    26401 agagccagca ggttggcatc agatgccggc gttcccgtaa ataaaatcgc aactgaaccc
    26461 ggaggacatc cctccagccg caggctaaaa gggacgagtg ccgtggtatc gccagcgttc
    26521 agtaattgtg tcgtaggcca tctgcctaaa tctaccgtct tatcaatatc cgctgtgttt
    26581 acggtacagg agaaatcaac aacgttaccg tgcaaattga tattaatcgt tcctaaaggg
    26641 tcagctgccc atccactgga actccacagt aacccgcaga aacagctaaa gaatactctt
    26701 ctcattctcc gtacctcatt gataatcgac gcgtaaatac cccagcgcgc taaacggccc
    26761 ttcggtcggt ttttgaccgg taatactgat aggccaggcg cgaagtgtga cattggctgc
    26821 cgcagctgca tccagacgga aaggaataac gctattgaga tcgttaggcg tgatcggcgt
    26881 atcgttctga tcggcgacaa taaaacctaa atcctgattg tccgacacca tcgcctgacc
    26941 agaaacggca ctggcttcca gacgcattgt taaataagcc tgcgcagcaa cattcgtaca
    27001 tttgaccgca atgctcttgg tttgcggcat gacaccagca ggtcgattac ccggccctgc
    27061 cgcactaaat aacgatgcgc cgatatcacc aaaatcaaat tcaacaatct gcccggcatt
    27121 taattcgcag ttttgcggta cttcaacccg gccaccaaaa ctaatggtat aaacaggtgt
    27181 aaccaacgga tcgtaaggcg ttgatgtcac atacacggta aacatcacct gtctggggat
    27241 ctccaccatg ttaataaagg gccgaataac ctttaaacga aatatcaaac gagaatccat
    27301 caccccgaac ggaaggtttt gcgaaacgtt aggatctgtt cccatacgaa tataattttg
    27361 cgggggaaat tgaatatcca tcacgctatc aaccagactc atcgcaccta at
//
LOCUS       DAZFFG010000062        27234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFFG010000062 DAZFFG010000000
VERSION     DAZFFG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.062"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..657
                     /gene="ybeU"
                     /locus_tag="ACHJ7J_002878"
     CDS             <1..657
                     /gene="ybeU"
                     /locus_tag="ACHJ7J_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5001090.1"
                     /translation="ILNQGCKPAHDSPKMYTGIKEFDLSSSWGINNRDDLIQTIYQMT
                     DDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDY
                     VRMGFLSRVGVLNKWLTEDESLWLQSRVYVRAHHYYHSWMHYFSAYSLGRLYWQSSQC
                     EDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL
                     "
     gene            654..2105
                     /gene="djlC"
                     /locus_tag="ACHJ7J_002879"
     CDS             654..2105
                     /gene="djlC"
                     /locus_tag="ACHJ7J_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO5001091.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGIDEEEREAFFEAI
                     QAGDCFDFLSLLEYPIALQNQTVEYYFALERCCRYHPDYVTAFLAMAGPWLIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQTEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWGSYPSTQADNLLLQWSAQHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2165..3835)
                     /gene="hscC"
                     /locus_tag="ACHJ7J_002880"
     CDS             complement(2165..3835)
                     /gene="hscC"
                     /locus_tag="ACHJ7J_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO5001092.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWQLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIEIP"
     gene            complement(3920..4855)
                     /gene="rihA"
                     /locus_tag="ACHJ7J_002881"
     CDS             complement(3920..4855)
                     /gene="rihA"
                     /locus_tag="ACHJ7J_002881"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO5001093.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(4973..5698)
                     /gene="gltL"
                     /locus_tag="ACHJ7J_002882"
     CDS             complement(4973..5698)
                     /gene="gltL"
                     /locus_tag="ACHJ7J_002882"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO5001094.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5698..6372)
                     /gene="gltK"
                     /locus_tag="ACHJ7J_002883"
     CDS             complement(5698..6372)
                     /gene="gltK"
                     /locus_tag="ACHJ7J_002883"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO5001095.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITITAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKTDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6372..7112)
                     /gene="gltJ"
                     /locus_tag="ACHJ7J_002884"
     CDS             complement(6372..7112)
                     /gene="gltJ"
                     /locus_tag="ACHJ7J_002884"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO5001096.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMICLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7282..8190)
                     /gene="gltI"
                     /locus_tag="ACHJ7J_002885"
     CDS             complement(7282..8190)
                     /gene="gltI"
                     /locus_tag="ACHJ7J_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO5001097.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(8587..10125)
                     /gene="lnt"
                     /locus_tag="ACHJ7J_002886"
     CDS             complement(8587..10125)
                     /gene="lnt"
                     /locus_tag="ACHJ7J_002886"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO5001098.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFASVLMSLR
                     QRRK"
     gene            complement(10150..11028)
                     /gene="corC"
                     /locus_tag="ACHJ7J_002887"
     CDS             complement(10150..11028)
                     /gene="corC"
                     /locus_tag="ACHJ7J_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO5001099.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11118..11585)
                     /gene="ybeY"
                     /locus_tag="ACHJ7J_002888"
     CDS             complement(11118..11585)
                     /gene="ybeY"
                     /locus_tag="ACHJ7J_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO5001100.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11582..12622)
                     /gene="ybeZ"
                     /locus_tag="ACHJ7J_002889"
     CDS             complement(11582..12622)
                     /gene="ybeZ"
                     /locus_tag="ACHJ7J_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO5001101.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(12775..14199)
                     /gene="miaB"
                     /locus_tag="ACHJ7J_002890"
     CDS             complement(12775..14199)
                     /gene="miaB"
                     /locus_tag="ACHJ7J_002890"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO5001102.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14345..15520
                     /gene="ubiF"
                     /locus_tag="ACHJ7J_002891"
     CDS             14345..15520
                     /gene="ubiF"
                     /locus_tag="ACHJ7J_002891"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO5001103.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRSKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(15674..15748)
                     /locus_tag="ACHJ7J_002892"
     tRNA            complement(15674..15748)
                     /locus_tag="ACHJ7J_002892"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15714..15716),aa:Gln,seq:ctg)
     gene            complement(15786..15860)
                     /locus_tag="ACHJ7J_002893"
     tRNA            complement(15786..15860)
                     /locus_tag="ACHJ7J_002893"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15826..15828),aa:Gln,seq:ctg)
     gene            complement(15909..15985)
                     /locus_tag="ACHJ7J_002894"
     tRNA            complement(15909..15985)
                     /locus_tag="ACHJ7J_002894"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15949..15951),aa:Met,seq:cat)
     gene            complement(16001..16075)
                     /locus_tag="ACHJ7J_002895"
     tRNA            complement(16001..16075)
                     /locus_tag="ACHJ7J_002895"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16041..16043),aa:Gln,seq:ttg)
     gene            complement(16110..16184)
                     /locus_tag="ACHJ7J_002896"
     tRNA            complement(16110..16184)
                     /locus_tag="ACHJ7J_002896"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16150..16152),aa:Gln,seq:ttg)
     gene            complement(16208..16292)
                     /locus_tag="ACHJ7J_002897"
     tRNA            complement(16208..16292)
                     /locus_tag="ACHJ7J_002897"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16256..16258),aa:Leu,seq:tag)
     gene            complement(16302..16378)
                     /locus_tag="ACHJ7J_002898"
     tRNA            complement(16302..16378)
                     /locus_tag="ACHJ7J_002898"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16342..16344),aa:Met,seq:cat)
     gene            complement(16758..18422)
                     /gene="asnB"
                     /locus_tag="ACHJ7J_002899"
     CDS             complement(16758..18422)
                     /gene="asnB"
                     /locus_tag="ACHJ7J_002899"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO5001104.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            18670..18768
                     /locus_tag="ACHJ7J_002900"
                     /pseudo
     CDS             18670..18768
                     /locus_tag="ACHJ7J_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18819..19571)
                     /gene="nagD"
                     /locus_tag="ACHJ7J_002901"
     CDS             complement(18819..19571)
                     /gene="nagD"
                     /locus_tag="ACHJ7J_002901"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO5001105.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(19619..20839)
                     /gene="nagC"
                     /locus_tag="ACHJ7J_002902"
                     /gene_synonym="nagR"
     CDS             complement(19619..20839)
                     /gene="nagC"
                     /locus_tag="ACHJ7J_002902"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO5001106.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            complement(20848..21996)
                     /gene="nagA"
                     /locus_tag="ACHJ7J_002903"
     CDS             complement(20848..21996)
                     /gene="nagA"
                     /locus_tag="ACHJ7J_002903"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5001107.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            complement(22056..22856)
                     /gene="nagB"
                     /locus_tag="ACHJ7J_002904"
     CDS             complement(22056..22856)
                     /gene="nagB"
                     /locus_tag="ACHJ7J_002904"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5001108.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            23189..25135
                     /gene="nagE"
                     /locus_tag="ACHJ7J_002905"
     CDS             23189..25135
                     /gene="nagE"
                     /locus_tag="ACHJ7J_002905"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO5001109.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            25403..27067
                     /gene="glnS"
                     /locus_tag="ACHJ7J_002906"
     CDS             25403..27067
                     /gene="glnS"
                     /locus_tag="ACHJ7J_002906"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO5001110.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
BASE COUNT         6548 a         6936 c         7083 g         6667 t
ORIGIN      
        1 atacttaatc agggttgcaa acccgcgcat gactcgccaa aaatgtatac cgggataaaa
       61 gaattcgatc tctcatcatc ctggggaata aataatcgtg atgatttaat tcagactatt
      121 tatcagatga ctgatgatgg tcatgctaat gatttagctg gcttatatct gacatggcat
      181 cgctcttccc ctgaagagtg gaaagcatta attgctggtg gctcggagag agggttgatt
      241 tatacccaat tcgttgccca aaccgctatg tgctgtggag aagggggaat caaagcttgg
      301 gattatgttc gaatggggtt tctgtcacgg gtcggtgtgc tcaacaagtg gctgacggaa
      361 gacgagagtt tatggctgca atcgcgcgtc tatgtacgag ctcaccatta ctatcacagc
      421 tggatgcact atttttctgc ctattcacta gggcgtctct actggcaatc ttctcagtgc
      481 gaagacaaca cttcgctacg cgaggcgcta accctgtaca aatacgatag cgccgggagt
      541 cgtatgttcg aagaactggc agcgggaagc gatcgtttct atgccacgct tccctggcag
      601 ccattaactg tccagcctga atgcccggta acgcttaagg atgtgagcga cctatgaaaa
      661 catgttggca aatcctcgaa attgaaagca cgacgcaaat agacattatc cgccaggctt
      721 atcttgctcg cttaccgtta tgtcatcccg aaaccgatcc gcaggggttt aaagcattac
      781 gccaggccta tgaagaggcg ctgcgactgg cggtaaatcc tgtcgaggaa gcagatgatg
      841 aagaaaaaga tgccgccgct gaacatgaaa tactacgtgc attcaggaca ttactggatt
      901 cagaaagtga tcgttttcag ccttccgcct ggcagaaatt tattcagcaa ttaaatacct
      961 ggaacatgga ggatgtcgat caattacgct ggccgctgtg tgcaatcgct atagaagcgc
     1021 gatatctttc attaaattgt gcttctttgc tggcagagcg tttgaactgg cattcgttta
     1081 atgacagcga aggaatagat gaggaagaaa gggaggcttt ttttgaggcc attcaggctg
     1141 gtgattgttt cgatttcctt agccttctgg aatatcccat tgcgttgcag aaccagactg
     1201 ttgaatatta cttcgcgctg gaacgttgct gtcgttacca tcctgactat gtcactgcgt
     1261 ttttggcgat ggcaggtccg tggttaattc ctgatgatgc gaagttgcat cgcaaactgt
     1321 tgcgctggta cagctcggtg caaacaggta tggcggaact cattcctgtt gcccaacagt
     1381 ggcaaacgga agaaccagaa agcgaagatg cccggtatta ccagtgtgca caacgtttgt
     1441 actgcggcga gggggaaagc ctgcttgccg atctctgcgc gtactgggga agttacccat
     1501 ctacacaagc cgataatttg ttgttgcagt ggagcgcaca acattgcccg gattatttcg
     1561 cgttattagt gatggttatc gaagcgcgga gcatggtaga tgcgcaaggt aaaccgttga
     1621 aatatgttcc tggtgagagc gcccggacgc gtctgttatg ggcggaaatt ttacatagcg
     1681 gaaaactatc gccgttaggt caatcgttta ttgagtcgct attcttcaaa cgcaaagcat
     1741 gggcgtggtg gaaatcgaga gtcggtagcg agacagaaga agattcaccg ttactggatt
     1801 tgtatcgggt agcggaacag gtagtacttg aagcgtttcc gaaacaagag atgctggccc
     1861 gtcttaatac aaggctggaa ggcggagatg ctcatccatt agaggccatt atcacccgga
     1921 tgctgttgac gaaagtaaaa ctcgagccgg aggatgaaga tgtcgatgag ccaacacctg
     1981 aaaaccatga agaaaaaaat gatgaggatg aaaaaccaca gagcattacc agcattatca
     2041 aaatcagctt aacggtgctg gtgataggtt atgctctcgg caaaatcgcg atgttgttta
     2101 gttgacgatg tgttttttgc gccgatgccg gatgcagcat ttacgccgca tccggcagcc
     2161 gtgattatgg gatctcaatg gctaaataaa cgctcgcccg ccgcctgacc tcatcgacgc
     2221 gggccatatc gtttgactgc agcgccgcat caaaatcggt gatcacccgg ccaatctctt
     2281 ctcgctcgtc acccagcgcc cgcgcccaca actcttccag tttggcctta aaggtgcggt
     2341 tgatgagcat atcgcgcgga taaattttca atgcggataa ccgcgtccga ctctcttcaa
     2401 tctgctgcgc gctcaatgtt accgggctgt ggttaatcac tctggactta acgctgccgt
     2461 cttccagaag cacgtcaact tcaagcaaac cgttgatatc ataactaaag cgaatatcaa
     2521 tcgactgata agccccggtt ctcttcaacg gcacatcgaa ggattccacc agaatgttgt
     2581 ttttaacttt gtggttttct ccctgataga cgttaaccgt aatagaatcc tgttccgggt
     2641 gcatggttga ataagtttct acacgcgaca cgggcacagt ggtgtttcgt tcaataatcg
     2701 gcgagaaaat gccggaaacg ccctggcggt taacctcaac gcccaacgag taagggcaaa
     2761 tatcagtgag gatcacttct tcaatatctt cactgcgtaa gcggcaggca gcctggattg
     2821 ctgcgcccag cgcgacaatg gtgctcggat cgtaactttg atacggtaat ttgccaaaca
     2881 gacgcacggc gattcgctgc accagcggca tttgtgacgc gccgccaacc agcaccagac
     2941 tgtcgatttg actcggcttc agacgcgcat cgcgcaacgc ctgttcaatc ggcactcgca
     3001 agcgattgag cagcggcaac cacaaatctt ccagttcgtt ctcgtaaaat tcgcattccc
     3061 gcgtttcatc ctggtactgc cagcgaatgt gcattggcga ttgattgcta cattttgccg
     3121 cttccacaca ggcatacaag gctgccagtt cactctcgtt aagcgtggtc ctggcgacat
     3181 ccgcgcgttt taaaacctca tcgaccagca tatgggtaaa atcttcgcca ccgaggaagt
     3241 tgtcgccagc ggaggcgtga acttcaatca ccggcgtggc gtactcaagc accgtaacgt
     3301 caaacgtgcc gccaccgaga tcaaacacca gcgaacgggt attttgttgg gtatgcaggc
     3361 catacgccat cgcagctgct gtgggttcat taattaagcg taccgcattt aaaccggcta
     3421 actccgctgc taaacgggta tgcttgcgtt gttcatcgct gaaataagcc ggaacagaga
     3481 tcaccacatc tttaatcgga cgttgcagaa attcttcggc atcttctttt aatgagcgta
     3541 ataccaaaga ggacagttct ggcgcgttaa atgtgtcgct gcctaactgc cagttggtat
     3601 tactgcccat tgcacgttta aataacgctg ccgttttatc cggatgcgaa gtacgccgtg
     3661 atacagccgg ttttccgact aaaatatgat tattttcatc catgctaatt atggatggtg
     3721 ttaaatattc accgaactta tttggaatta attgcgcggc accgtctttc cagacggcaa
     3781 ttaaactatt ggtagtaccg agatcgatac caatggcgag ttctgcatta tccattgcat
     3841 ttatatcttg aaaaaaaaaa gagataaaca gattaagacc acccaggtaa cgcgtggtga
     3901 tcttaatcaa tgacgtgtgt taagcgtaaa atttcagacg atcagccagt aaatcaacaa
     3961 agccctgacg atcaacatcg accattacag tggcattcgg tttattgcca gtcagataat
     4021 aataatcaac aaccgtcatc ccctgggtat atttcccctg tgtttccacg ccaacccagc
     4081 gctcaacagt ggtaaacaac tcgggtttca acagccaggc gatggtgcat gggtcatgca
     4141 gcggtgcgcc gacaaagccc catttttcgt ctttatgata ttcgaggaag aaatccagca
     4201 gttcggcaac aatggttgaa accgggttac caatcgcgcg gaaacgctcg gtgtcctcaa
     4261 cgtggatttg cgctttgtga gtaacatcca gaccggccat caccaccggg atccctgact
     4321 ggaagacaat ttctgccgct tccgggtcaa cgtaaatatt aaattcagcc gcaggcgtcc
     4381 agttaccaag ccccattgcg ccgcccataa tcacgatacg ggcaattttg ctatgcagtt
     4441 ccggatggct attgagcagc aaggcaacgt tagtttgcgg tccggtagaa acaatggtga
     4501 caggttccgc actttcacgc agcgttttcg ccattagctc taccgccgta cagttttgcg
     4561 gtgcgaacgc cggttccggt aatgccgggc cgtcgagacc gctttcgccg tgcacattgt
     4621 ccgcgataat caactcacgc attaacggtt ttaccgcgcc gcctgctacc ggaatatcgg
     4681 tgcgattaag caaggtcagc atacgcagaa cattgcgtaa ggttttttct ggtgtctggt
     4741 ttccggcgga agacgtaatt gctttgacat caagctctgg tgaggcgagg gcgagaacta
     4801 ttgcgatagc gtcgtcatga cctgggtcgc aatctaacag aattggcagt gccattgttg
     4861 ctccttgttg tgtgcttctt tgcgacaagg gtaacgccag gatgtaacag atacgagggg
     4921 cgaaacgata aagcgtgaga tggcgcgcaa ttgggtatgc gcgccagagt gattaatgca
     4981 ggattttcgc gaggaagtct tttgcgcggt ccgatttcgg atcatcgaag aacgcatctt
     5041 tcggcgagtc ttcaacaatt ttaccctcat ccataaagat cacccgattc gccactttac
     5101 gggcaaagcc catttcgtgg gtcaccacca tcatggtcat cccctcgttc gccagttcca
     5161 ccatcacgtc cagtacttcg ttgatcatct ccgggtctag tgccgatgtg ggttcgtcga
     5221 acagcatcgc aataggatcc atacacaatg cgcgagcaat tgccacacgc tgctgctggc
     5281 cgccggaaag ctgcgccgga aatttattgg catgtgcgga aagtcccacg cgctccagca
     5341 atttcaatgc tttttcacga gccggcgctt tatcgcgttt aagcactttc acctgcgcca
     5401 gggtcaggtt ttcgataatt gacagatgag ggaacagctc gaaatgctgg aacaccattc
     5461 ccacacggga acgcagcttt gccagatcgg ttttcttgtc gttaaccacg atgccatcga
     5521 cggtgatttc accttgctgc accggttcga ggccgttgac ggttttaatc agcgttgatt
     5581 tgccggagcc agacgggccg caaaccacca ccacttcgcc ttttttcact tcggttgagc
     5641 agtcggtcag cacctgaaag tgaccatacc attttgaaac atttttcagg gtaatcatta
     5701 tgctgtcctt cttttcaagt agctgaccaa caacgacgca ctaaggctaa tcacgaaata
     5761 aacaaatccg gcaaacagga tcatctcgac ctgcgtacca tcacgctcac caatggttga
     5821 ggcggtacgg aagaaatcgg ccagcgataa cacatatacc agcgaagtat cctggaacag
     5881 tacgatgccc tgagtgagca gcagcggcac catcgcgcga aacgcctgcg gcagaataat
     5941 cagtttcatc gactgccagt gggtcatccc cagcgccagc gcggcgctcg actgaccacg
     6001 agaaatactt tgaataccgg cacggataat ctctgaatag taggccgctt caaacatcga
     6061 aaacgccacc atcgccgaaa ttaaacggat atcggttttc ggcgataatc ccagcacgtt
     6121 ttgcagaaaa cccggtacga tcaggtaaaa ccacagcagg accatgacta atggaattga
     6181 gcggaacacg ttaacgtagg ctttggcaaa ccacgctacg ggcgcaaagc tggacaaacg
     6241 catcaccgca agcatagtgc cccacaaaat accaatcact accgccgtga tggtaatttt
     6301 cagggtgatc accagcccat ccagcagata cggcagggaa gggacaatgg aactccagtc
     6361 aaactcgtac attatttgcc ccccatgttg ccaggcaggc gaactttacg ttcaaccagc
     6421 gtcatcacca gcatgataaa agcgttaatc aacacataag caagcgtaat ggcggtaaac
     6481 gactcccagg cgtgggccga gtaatcgagc aatttacccg cctgcgctgc catatccacc
     6541 agaccgatag tcgaggcgat ggcggagttt ttcaccaggt tcatcatctc ggaagtcatt
     6601 ggcgggacaa taacgcgata agcgttaggc aacagcacgt agcggtaagc ctgcggcagc
     6661 gtcagcccca ttgccagcgc ggcattcttt tgcccacgcg gcagcgactg aatggcggcg
     6721 cggacctgtt cgcaaacacg ggcggcggta aacagcccca ggcagatcat ggaggagagg
     6781 aaaaactgaa tatttgggtc cagttccgcc ttaaaccaca tgccgatttt ctccggcagc
     6841 agctccggga tcaccagata ccaggtaaag aattgcacaa tcaacggcac gttgcggaac
     6901 agttcgacat acaacgtacc cagaccggag agaaaacggt ttggaacggt acgtaaaatg
     6961 ccgaaaaatg aaccgacgag gaaagcgata atccaggcgc agatcgacaa agcgatcgtc
     7021 acctgaaaac cactccagat ccaaccaaga taggtggtgt tgccgaacgg ggcttgttgt
     7081 aaaaaaatac cccagttcca gtctatagac ataaatctac tccagaaaaa agagggtagc
     7141 agcgttaact gctaccctcg aagattgtta cccagcgtat tgcggttttc aggcccgatg
     7201 gggaacgacc atcaggcgta tagtctgtcc gtgctacgta acaatcgaga gggctagaat
     7261 ttccgcccct ggttcttgta attagttcag tgctttgtca ttcggttctt tgaacagtgc
     7321 tttcatttcg tctgacagtt cgaaattcat gttcaggttt ttcggcggaa ttggattttt
     7381 gaaccattta tcaaaccatt tttccgcttc accggaggtc tgcacctgag cgatggtgtc
     7441 atccatcagc tttttgaact gcggatcgtc tttacgcaac atgcaaccgt aggcttcctg
     7501 agactgcggc ttgccgacga tatcccagtt gtctggtttc ttcgctttcg cacgttcacc
     7561 ggccagcagg gcatcatcca tcataaaggc aacggcacga ccgctttcca gggtgcggaa
     7621 agagtcaccg tgatctttgg cgctgatgat gcgcatattc attttttgct cttcattcag
     7681 tttgttgagc aaaacttcag aggtagtgcc ggaagtgacg actacggctt tgtctttcag
     7741 gtcggcaaaa tctttgatat cgccgccctt tttggtcagc aggcgcgtac cgaccacgaa
     7801 aatagtgtca gagaaagccg cctgtttttg gcgttcgacg ttattggtgg tggaaccaca
     7861 ttcaaaatcg aaagtgccgt tttgcagaag aggaatacgg ttttgtgagg tgatcggaat
     7921 cagtttcacc tgcaagtccg gtttgttgag tttctttttc actgcttcaa caatggcgtt
     7981 ggagtaatcc tgcgaataac ccaccacttt ttgctgattg tcgtaataag agaaaggtac
     8041 tgaagattca cggtgaccga cgacaatcac gccgtttttg gcgattttgt ccagcgtact
     8101 gcccgctgcc ggggcggcgt catctgcctg tgccagtcct gcggaaagcg caagggccag
     8161 gattgctgtg gcaggtttac gtaattgcat atccaactcc tttatcttct gcgttaagaa
     8221 cgcatggata cccgttgtga gtgtttgtgt tgttatcatc tgcaacttta ttgtgcagtg
     8281 ttgtgcctgt taggtaacat ttagtttggc taaatgtaaa gatattgctg ttttattgtt
     8341 tatatttgcg aggtgcaccg caccattccg aagcaaaatt cttaaaatgc actcttttgg
     8401 tgctaccggt ggattgctgt ggtgcaaccg ggttgtactg atgctggtta aagctttgcc
     8461 ttgtataaca aagcaataga tgtgccaaag ttggatagga gaatattgtt atccggataa
     8521 tgcactgatg ccgcatccgg tgagcgtgcc gaaatatggg atgtattccg gcacgataag
     8581 aagggattat ttacgtcgtt gacgcagact catcagcaca gaggcaaaac caaacagtgc
     8641 cgtcagcacc cacagcggcc agttgccggt acgtgcgtat ggtgtgagtc cggtggtcgg
     8701 cgtcacgtta gtggttaaca cctcgcgggt gaactgcggg atcatcgcct gaatctcacc
     8761 ctgcgggcca atcaccgccg taatgccgtt gttggtgctg cgcaacagtg ggcgcgccag
     8821 ctccagcgca cgcattcgcg ccatctggaa gtgctgccat ggaccaatag acttaccaaa
     8881 ccacgcatcg ttggagatag tcagcagata gtcggtatcc gggcggaagt tatcgcgcac
     8941 ttgctcgccg agaatgatct cgtagcaaat agccgcagta agctcaatac catttgccga
     9001 cagcggcggc tggatatatg gcccacggct gaacgatgac atcggcagat caaagaacgg
     9061 tgctaacgga cgcagaatcg actccagcgg gacaaactcg ccaaacggca ccagatggtt
     9121 tttgttatag cgatcggctg attcgtagct gtacggtgca cctttaccca gtgtgatgat
     9181 ggtgttgtag gtatcgtaac ggttctgctt attaagacgc gcgtcaacaa tcccggtgac
     9241 cagcgagcta cctttatcac gcaactcacc gtccagagct ttgaggaacg gttgctgatt
     9301 aatttccaga tcggttatcg ccgactccgg ccagataatc aacgatgatt tgcccatcag
     9361 cggtgccgtt gcgttgtagt aaatcttcag cgtattaaga agctggcctt cgtcccattt
     9421 cagcgattgc ggaatatcgc cctgaaccat cgaaacctga atggttttct ccggttgcgg
     9481 ggtaaaccat tggatgtaac gcagcgggaa gggtagggca aacagcacga cggccactac
     9541 cagcggacgc cagttgcgtt tgaccaacgc cagtgccagc aggccactaa ccatcatcag
     9601 caggaagtta atggcttcca cgcccattag cggtgccagc ccttttaacg ggccatcaat
     9661 ctggctatag ccgaactgta accacgggaa gccagtcagc acccaaccgc gcagaaactc
     9721 ggtcacttgc cagagagcag gagcggcaat cgccacgcgc agccaggtgg ttttcggcca
     9781 caggcgtgac agcacgccag caaacagtcc ggtatacagc gacaaatacg ccgccagcag
     9841 taccaccagg aagatgttaa ccgggccagg cattccgcca aaggtcgcga tgctgacata
     9901 gacccagtta ataccgctgc caaagagacc aaatccccag caaaagccaa tagcggcaga
     9961 ctggagtgga cggcggttaa aggtcaacgc ctgaagcccc atcagcgaaa taatcgccgc
    10021 aggccagaca tcgtaaggag agaaggccag cgttccgcag gcaccgaata ataacgccag
    10081 cagcaggcga atgcgctggc gttcaattaa tgaggcaaaa gccatgtagt tatctatcca
    10141 gtttcggttt tattcatcca gcttcggctg gggtgagtca tccgggattt tgacatgaac
    10201 ctgaataata cgccgactgt cggccatcgc cactttgaac tggtaaccgt cgatgtcgat
    10261 ggtttcgcca cgtgccggaa gatgcccaaa tgcctgcatc accagaccac cgatagtgtc
    10321 gacttcttca tcgctaaagt gggtgccgaa cgcttcgttg aagtcttcaa tggaagccag
    10381 tgcgcgcacg gtccaggtat gacgactcag ctgacggaag tcgatatcat cttcttcgtc
    10441 atattcgtct tcaatctcac caacaatcag ttccaggatg tcttcaatgg ttaccagacc
    10501 ggaaacccca ccgaattcgt caataacgat cgccatgtgg taacgctgag agcgaaactc
    10561 tttcagcatc cggtctacgc gcttactttc aggaacgaca actgcctgac gtaacacttt
    10621 gtccatgctg aaggcttcag catcgctgcg cataaacggc agcaagtctt tcgccatcag
    10681 aatcccttca atgtgatctt tgtcttcgct aatcaccggg aaacgtgagt gggcggactc
    10741 gatgatgaca tcaaggcatt cgtccagcgt ctggttgcgt ttcagggtaa tcatctggga
    10801 gcgggggatc atgatgtcgc ggacgcgttg gtctgcgatg tccatcaccc cttcgagcat
    10861 atcgcgcgta tcttcgtcga taaggtcgtt ctgcccggaa tcacggatca gcgccagcag
    10921 ttcgtcacgg tttttcggtt caccgtggaa aagttggctg agtaacaggg agaaaaatcc
    10981 cttcttgttg cttatcgtgt cactactgtg tgaattgtcg tcgctcatgg cgtcgtatgg
    11041 gttctcatgt tagttaatca aaacgccgtc gttaatcacc aacggcgggg acgtctgcca
    11101 gtcaaatggc tggcaaatta ttctttctcg gcaatgtacg gatcctcata gcccagagca
    11161 agcataatct ctgtttcgag ggcttccatt tcttctgctt cgtcatcttc gatgtgatcg
    11221 taacctaaca aatgcagact gccgtgcacc accatatgcg cccagtgcgc ctccagtggt
    11281 ttgccttgct cctgagcttc cttctcaacc acctgacggc agataaccag atcgcccagt
    11341 agcgacattt ccatgccagg cggcacttca aacgggaagg agagcacgtt ggtcggctta
    11401 tccttaccgc gataggtcag attgagactg tggctttcgg cggtatcgac cacgcgaatc
    11461 gtcacttccg actcttcctg aaactgcggg atcaccgcat tcagccatgt ttgaaactgg
    11521 ctctcttccg gcaatccgga gttatcttca catgccagtt gtaaatcgag gatcacctga
    11581 ctcatttttg ttcctgttct tcgcgcttgc gttctgccgc cagcgccgct ttacgttttt
    11641 gttcggcttc ttcccaggct tcataggcgt taacgatacg cgccaccacc gggtgacgaa
    11701 ccacgtcttc gctgtggaag aagttaaagc tgatctcttc gacatcggcc agcacttcga
    11761 tggcatgacg taagcctgat ttagtattac gcggcaggtc gatctgtgtg acgtcgccgg
    11821 tgataaccgc ttttgagtta aaaccgatac gggtcaggaa catcttcatc tgttcgatgg
    11881 tggtgttctg gctctcatcg agaatgataa aggcgtcatt cagcgtacga ccacgcatat
    11941 aggccagcgg cgcgacttca ataacgttgc gctcaatcag tttctcgact ttctcaaagc
    12001 ccagcatttc aaacagcgcg tcgtacagtg ggcgcagata cgggtctact ttctggctta
    12061 aatcgccagg caggaagccc agtttctcac cggcttcgac cgccggacga gtcagcagaa
    12121 tacggcgaat ttcctgacgc tccagggcat caactgccgc agccactgcc aggtaggttt
    12181 tacccgtacc cgccgggcca acgccgaagg taatgtcatg gtcgagaata ttggcgatgt
    12241 actgcgcctg gtttggcgtg cgcggcttaa ttacgccgcg tttggttttg atattgaccg
    12301 ctttgccgta ctccggcacg ctctccgcac tttgctccaa tacccgcgct tctttaatcg
    12361 caaggtggat ctgttccggt tcaatatcct gaatctgacc gcgcatcggg gcagtatcga
    12421 catacaggct acgcagaatg tctgccgcag cggtgacgca aatcggacgg ccggtcagtt
    12481 taaagtggtt atcgcggcga ttgatctcga tgccgagacg gcgttcgagc tgcttgatgt
    12541 tgtcatcaaa cgggccgcac aggctcaaca gacgcgcgtt gtctgctggc tccagggtga
    12601 tttcgcgagt gtctatgttc aaaccgttcc tcttatctgt atgccgccgg aagctgaaca
    12661 ttcaccggcc tataaggaaa ttattcacgc cacaggaaaa aggcgcaagc attgcgataa
    12721 agatggggat aaagagagaa aaaacaaggc ccaccggaac ggcgggcctg agaattacgg
    12781 ctgataataa cccacgccaa ggtcgttttc tttgcgagta cgggctatca ccgattccgg
    12841 tgtttctgcc acgcgcagac ccatttcatc ttcagttcgc accactttac cgcgcagaga
    12901 gttcgggtag acgtcggtaa tttctacatc gacgaattta ccgatcatat ccggcgtgcc
    12961 ttcgaagttg accacgcggt tattttccgt acgcccggaa agctccatga tgctcttacg
    13021 cgatgtacct tctaccagaa tacgctgggt ggtgccgagc atccggcggc tccacgccat
    13081 cgcttgctga ttaatgcgct cttgcagaat atacagacgc tgcttcttct cttcttccgg
    13141 gacatcatca accatatcgg cggctggtgt acccggacgt gcagagaaga taaagctgta
    13201 gctcatgtcg aaattgacgt cggcaatcag cttcatcgtt ttctcgaagt cttcggtggt
    13261 ttcgccaggg aagccaacga tgaaatcaga actgatctga atatctggac gcgccgcacg
    13321 cagtttacgg atgatcgctt tgtactccag cgccgtatgg gtacgtccca tcaggttcag
    13381 aatgcgatcg gaaccgctct gtaccggcag atgcaggaag ctcaccagct ccggcgtgtc
    13441 gcgatacact tcgatgatat cgtcggtgaa ttcgatcgga tggctggtgg taaagcgaat
    13501 acgatcgatc ccgtcgatcg cagcaaccag acgcaacaga tcggcaaacg atccggtggt
    13561 gccgtcgtag ttttcaccac gccaggcgtt cacgttctga ccgagcaggt tgacttcacg
    13621 cacgccctga gccgcaagct gggcaatctc aaacagaata tcgtcggacg gacggcttac
    13681 ctcttcacca cgggtgtaag gcaccacgca gtaggtgcaa tatttattgc agccttccat
    13741 gatggagaca aacgcggtcg gcccttcggc gcgcggttcc ggtagacggt caaacttctc
    13801 gatttccggg aagctgatat ctacaaccgg gctgcggtcg ccgcgcacgg agttgatcat
    13861 ctccggcaga cggtgcagcg tttgcggccc aaaaataata tcgacatagt gggcgcgctg
    13921 gcgaatgtgc tcgccttctt gcgatgccac gcagccaccg acgccgataa tcaggtctgg
    13981 attcttctct tttaacagtt tccagcgacc caactgatgg aagacttttt cctgagcctt
    14041 ctcgcggatt gagcaggtgt tcagcagcag cacatccgct tcttccgcca cgtcggtcag
    14101 ttgatagccg tgggtggcat ccagcagatc ggccatcttc gatgaatcgt actcgttcat
    14161 ctgacagccc caggttttaa tatggagttt tttggtcatc gacttgctct tgcgaaatag
    14221 tagccaggaa tgcagggcgc atagtgtaat gctttgctgc cgttgtgacc agtatgagcg
    14281 ttatcagccc ttgggggtaa aaatcctgta aacttaaagc atattgctaa caggatgatt
    14341 gaccatgaca aatcaaccaa cggaaattgc cattgtcggc ggaggaatgg tcggcggcgc
    14401 actggcgctg gggctggcac agcacggatt tacggtaacg gtgatcgagc acgcagaacc
    14461 tgcaccgttt gtcgctgata gccaaccgga cgtgcggatc tcggcgatca gcgcggcttc
    14521 ggtatcattg cttaaagggt taggggtctg ggatgcagta caggctatgc gttgccatcc
    14581 ttaccgcaga ctggaaacgt gggagtggga aacggcgcat gtggtgtttg acgccgctga
    14641 acttaagcta ccgctgcttg gctatatggt ggaaaacact gtcctgcaac aggcgttgtg
    14701 gcaggcgctg gaagcgcatc cgaaagtaac gttacgtgtg ccaacctcgc tgattgcatt
    14761 acatcgagat aatgatcttc aggagctgga gctgaaaggc ggtgaagtga ttcgctcgaa
    14821 gctggtgatt ggtgccgacg gcgcaaattc gcaggtgcgg cagatggcgg gaattggcgt
    14881 tcatgcatgg cagtatgcgc agtcgtgcat gttgattagc gtccagtgcg agaacgatcc
    14941 cggcgatagc acctggcagc aatttactcc ggacggaccg cgtgcgtttc tgccgttgtt
    15001 tgataactgg gcatcgctgg tgtggtatga ctctccggcg cgtattcgcc agttgcagaa
    15061 tatgaatatg gcacagctcc aggcggaaat cgcgaagcat ttcccgtcgc gtctgggtta
    15121 cgttacaccg cttgccgctg gtgcgtttcc gctgacgcgt cgccatgcgt tgcagtatgt
    15181 gcagccaggg cttgcgctgg tgggcgatgc cgcgcatacc atccatccgc tggcggggca
    15241 gggggtgaat cttggttatc gtgatgtcga tgccctgatt gatgttctgg tgaatgcccg
    15301 cagctacggc gaagcgtggg ccagttatcc tgtcctcaaa cgttaccaga tgcggcgcat
    15361 ggcggataac ttcattatgc agagcggtat ggatctgttt tatgccggat tcagtaataa
    15421 tctgccgccg ctgcgttttg tgcgtaatct tggtttgatg gcggcggagc gtgctggcgt
    15481 gttgaaacgt caggcgctga aatatgcgtt agggttgtag ccttacaaca ttgccggatg
    15541 cgtgccaacc gtaggtcgga taagacgcgg cagcgtcgca tccgacattg aaggataaga
    15601 cgtatcaaca tcgcattcga cattgaatga acgcagaaaa gcaaaaagct cgccgaagcg
    15661 agctttttta atgtggctgg ggtacgagga ttcgaacctc ggaatgccgg aatcagaatc
    15721 cggtgcctta ccgcttggcg ataccccaac tgggtgcact tacaaggtaa gtgtcttgaa
    15781 taaattggct ggggtacgag gattcgaacc tcggaatgcc ggaatcagaa tccggtgcct
    15841 taccgcttgg cgatacccca acaaattggt ttttgaattt gccgaacata ttcgacacat
    15901 tcagaatttg gtggctacga cgggattcga acctgtgacc ccatcattat gagtgatgtg
    15961 ctctaaccaa ctgagctacg tagccagatt gtttcttcga tggctggggt acctggattc
    16021 gaaccaggga atgccggtat caaaaaccgg tgccttaccg cttggcgata ccccaataac
    16081 cgggcggtga accgcttact cgaagaagat ggctggggta cctggattcg aaccagggaa
    16141 tgccggtatc aaaaaccggt gccttaccgc ttggcgatac cccatccgta caacgctttc
    16201 tggtgaatgg tgcgggaggc gagacttgaa ctcgcacacc ttgcggcgcc agaacctaaa
    16261 tctggtgcgt ctaccaattt cgccactccc gcaaaaaaag atggtggcta cgacgggatt
    16321 cgaacctgtg accccatcat tatgagtgat gtgctctaac caactgagct acgtagccat
    16381 cttttttttc gcgatacctt atcggcgttg cggggcgcat tatgcgtata gagccttgca
    16441 gcgtcaacct ctttttcaag gaaaattgct cgaaagtgac tgtttggtta ggttgcgaac
    16501 agcgtggcgc tatattcgtc aattattgtt tactttgtgt tttttcccac cctacagcca
    16561 ttcttttgtc atacaggatg aaattcggaa tttaacaata gtggtgggaa attaatctat
    16621 gaaatactgg cctacagtga tgagttgtca aacagtgatg tggcaaaccc ggaacatttc
    16681 cttactgcat atcagaatca acaagcacct caataactga aacagccccg gatttcaccg
    16741 gggctgtttc gcatttctta cttatacgcc gactggtgaa caccaaccgc gcgaccagac
    16801 ggatcgtcca tttttttgaa cgcttcatcc cattcgatcg ctttagctga agaacacgcg
    16861 acggaaggac cgcccggcac gcactcagcg gcgcttggaa gcgggaacag ttcttcaaag
    16921 atttcccggt acagatacgc ttcttttgag gtcggtgtgt tgtacgggaa gcggaagcgg
    16981 gcagtttcca gctgttgatc agaaacctgc tgcgccgcca cttctttcag ggtgtcgatc
    17041 caactgtaac cgacgccatc ggagaactgc tctttctgcc gccaggccac gcttgcgggc
    17101 aggtatgact caaaacattc acgcaggatg tgtttttcca tcttgccgtt accgcacatt
    17161 ttatcctgcg ggttaatgcg catcgccacg tcgaggaatt tcttgtcgag gaacggaacg
    17221 cgtgcttcca cgccccaggc tgacatcgct ttgttggcac gcgcgcagtc atacatatgc
    17281 agggccagca gtttacgcac cgtctcttca tgcagttctt tggcattcgg tgctttgtgg
    17341 aagtaaagat aaccgccaaa cacttcatca gaaccttcac cggacagcac cattttaatg
    17401 cccatcgctt tgatcttacg cgacattaaa tacatcggtg ttgaagcgcg aatagtggtc
    17461 acatcatagg tttcgatgtg gtaaatcacg tcgcggatgg cgtccagacc ttcctgtaca
    17521 gtgaagtgaa tttcatgatg cacagtgccc agatggtttg ccacttcctg ggctgctttc
    17581 agatccggtg aacccggcag acctacagca aaggagtgta actgcggcca ccaggcttca
    17641 gagcgttcct gatcttccac gcgacgggct gcgtatttct tggtgatagc ggaaataatt
    17701 gaggaatcca gaccaccaga aagcagcaca ccgtaaggca catcagacat cagatggctt
    17761 ttaacggaat cttccagagc ctgacgcagc tcgtttttgt cggtgacatt atctttcacc
    17821 gcatcgtagt cgaaccagtc gcgatgataa taagaacgga tttcgccgtc ctgactccac
    17881 aaatagctcc ccgccgggaa ctctttaatc gtgcggcata ccggcacgag ggctttcatt
    17941 tctgaggcca catacagctg accatgttcg tcatagccca tatacagtgg gatgatcccc
    18001 agatggtcgc gaccaatcag gtaggcatct ttttcgctgt cgtacagggc aaaggcaaac
    18061 atgccctgca agtcgtcgag aaattccggc cctttttcct gatacagcgc gaggatcact
    18121 tcacagtcag accctgtctg gaactgataa cgatcgccgt attcagcgcg caatgcctgg
    18181 tgattgtaga tttcaccgtt taccgccagt acgtgggttt tttgttggtt gtagagaggt
    18241 tgcgcccccg cgttaacgtc aacaattgac agacgttcgt gggcgagaat ggcgttatca
    18301 ctggcataaa taccggacca gtccgggcca cgatgacgca tcaggcgtga cagctcgagt
    18361 gctttcttac gcagctcaac tgcgtctgtt ttgatatcga atacgccaaa aattgaacac
    18421 ataaccttct ccgttaacct ggtatttgtt gcttgttgtg tttgcttgtt taaaaaaatg
    18481 ccgcaaagca gcactgtgcg caagcgattt ggcggtgaaa aaataaaaaa cgtaatggtg
    18541 attgctgatt tgtgaaaaag gggagggtgc ggtgatgtat ttattaatgg atcgataatt
    18601 tttggcgggt tttattgaat gtttatattt tacgggggcc aaattgctga caaagtgcga
    18661 tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca tgaaaattca
    18721 atatgttgca ggagctacgt aggcctgata agcgtagcgc atcaggcagt ttggcgtttg
    18781 tcatcagagc caaccacgtc cgcagacgtg gttgctattc agataacgtc gatttcagcg
    18841 accgatgggt aaatccagct ggggcggaac ggcatactgt cgatatcgtc gagcgacgaa
    18901 acaccagaaa gcaccagaat cgtctccaga cctgcctgga agccggccag aatatcggta
    18961 cgcaggttat cgccgacaat caccgtttct tccgaatgtg cctgcatttt gtttaatgcc
    19021 gcgcggataa tccacggact cggcttacca acatagaacg gtttgcgccc ggagattttc
    19081 tcaatccctg cacacaacgc gccacaggcc ggataaaaac cgcgcccgtg agtatccggg
    19141 ttggtggcga taaagcgtgc accgttagcg acgaaatagg ccgctttatg catcatgtcc
    19201 cagttgtagg aacgcgtttc gccaacaatc acaaaatcag ggttcacatc ggtaatagtg
    19261 aaaccggctt tgtacagttc atgaatcagt gcgccttcgc ccaccacata cgctttcttg
    19321 ccttcctggc gacgcaggaa atcggcggtc gccatcgcag aggtataaaa cacgctgtca
    19381 ggtacatcga caccggcagt ggcaaagcgg ttcgccagat cttgcccggt ctgcgaagga
    19441 tagttggtca gcaacaccag cggcaggcct ttatccataa tcccgtgcaa aaattccgct
    19501 gcacccggta cggcgacgtt atcgtgcatc agcacgccgt cgatatcgca aattacattt
    19561 ttaatggtca tggactaccc agaatattga caacaataag cgccactata aaagcacatt
    19621 aattttccag caaatgctgg agcaaaatac cgttgagcat ggcgcgtttt accagcgcaa
    19681 aagcgccgat tgccgagcgg tggtctagct cagaacgtac caccggcaga ttagtgcgaa
    19741 acgccttcag cgcctgagta ttaatgcagc tttcaatagc agggagcagc actttatcgg
    19801 cttcggtgat ttcaccggca ataacaattt tttgcgggtt aaacaggttg atggcaatgg
    19861 cgatggtttt gcccagatga cgaccgacat actcaatcac ttccgacgcc aggctatcgc
    19921 ctttgttcgc agctttgcag atagttttta tggtgcagtc gtccagcggc acgcggctct
    19981 ggtagccctg ctttaacaga ttcaacaccc gttgttcaat agcggcgttg gcagcgatag
    20041 tttccaggca gccaaagttg ccgcagtggc agcgttcacc cagcggttcg acctgaatat
    20101 ggccaatttc accgacgtta ccgttgcggc caataaaaat gcgcccgtta gagataatcc
    20161 cggccccggt tccgcgatgg acacgcacca gaatggagtc ttcgcaatcc tgacttgcac
    20221 cgaagtagtg ctccgccagc gccagactac ggatatcgtg accaacgaaa caggtcactt
    20281 taaaacgctc ttccagtgct tctaccagcc cccagttttc tacctgaata tgcggcatgt
    20341 aatgaatttt gccgctgtcc gggtcaacaa gtcctggcag gataacggaa atggcgatca
    20401 gctcgcgcag tttgcgctgg tagctatcaa taaactgagc aatggcattc agcagggcat
    20461 gttccagcgt ctgctgggta cgttccggca gcgggtaatg ttcttctgcc agcactttgc
    20521 tgctgagatc aaacagggtg atggtggcgt cgtgacgacc aagccgtacg ccgattgcgt
    20581 ggaaattgcg ggtttcggtg acgatagaga tagcgcggcg gcccccggtg gaggcctgct
    20641 gatcaacttc tttgatcagc ccgcgttcga taagctgacg cgtaattttg gttacgctgg
    20701 cgggggcaag ctggctttgc tcggcaatct gaatccgcga gattggcccg tactggtcaa
    20761 tcaggcgata aaccgccgcg ctgttaagct gttttacgag atcaacatta cctatctgag
    20821 cttgtccgcc tggtgtcata ctttctctta ttgagttacg acctcgttac cgttaacgat
    20881 ggtcttggtg attttaaaat caggtgtgaa tgcggtcagg ttggctactt tacctgcggc
    20941 gagtgtgccg agacgtttct caacgccaat cgcacgcgcc ggatagagcg tcgccatacg
    21001 cagcacttca tccagtgcga taccgcaatg ttcgaccaga ttacgcacgc cttcaatcat
    21061 ggttaaggat gaaccgctta acgtaccgtt ctcatccaca caaagtccgt tacggtagta
    21121 tattgtttta cccgcaaaaa tgaactgttc aatgttggca cctgctggcg cggtggcgtc
    21181 ggtaaccaga cacagtttgt cgcctttaag gcgtttagcg ttacgaatgt tggcgtaatc
    21241 aacatgcagg ccatcagcga taataccgca ataaatgtca gcttcatcga ggatcgcgcc
    21301 cgccaggccc ggttcacgac cggtaatata cggcatcgcg ttgtacagat gggtggcaaa
    21361 ggtaatcccc gcgcggaaac cggctttcgc ttctttcaac gtcgcgttgg agtgaccggc
    21421 agaaaccaca atcccggcat ttgccagttt gctgatgact tccgcaggaa ccatttccgg
    21481 tgccagggtc actttggtaa tgacgtcggc gttctcacac aggaaatcga ccagcgcggc
    21541 atcaggctta cgcacaaaat tcggattatg ggtacctttt ttcaccagat tcagccacgg
    21601 accttccaga tgcagaccta acgcctgatt cggatgtttt gccaggtact cgcgcataac
    21661 gcgcacgccc tgtttcatca gctcatcgct ggtggtgata agcgtcggca gatagttagt
    21721 acagcctgat ttctcattgg ctttctgcat gatttccagc gtttccacgc tgaccgcttc
    21781 agcggtgtcg ttaaactgta cgccgccgca gccgtttaac tgcacatcga taaaaccggg
    21841 ggagagaatg gccccgttca gtgaacgttg ttcgatctct ggcggcagtt ccgctaccgg
    21901 acagacgctt ttaatcaggc catcagcgat aacaaccgcg tggtcatcaa gaaattcgtg
    21961 accggtaaag atccggccct gggttaatgc atacattctg acccccgatt ttaaaaaata
    22021 ttgccctgag caaggtgtca gggcagggat aacaattaca gacctttgat attttctgct
    22081 tctaattcat tgaaatatct taaagtctta actttcagct ccatggtgga aggctcatcg
    22141 cacaccatga tggctttcgg atgcagttgc agacagctga tggtccacat atggttcacg
    22201 caaccttcaa ctgcggcctg cagcgccagt gctttctggc tacccagcac cagaatcatc
    22261 acttcttcgg catccagcag tgtaccaaca ccgacagtca gggcatattt tggcacctga
    22321 ttgacatcgt tatcaaagaa acgagagttc gcgacgcgag tgtcatgagt cagggttttg
    22381 atacgagtac gagaagccag agaagacgcc ggttcgttaa atgcaatatg accgtcgtta
    22441 cctacaccgc ccataaacag atgaattttt ccgtaagaac ggattttttc ttcatactgg
    22501 cggcactcgg cgtcgatatc cggggcgttg ccgttgagaa ggttgatgtt ttctgctgga
    22561 atatcaacgt gatcgaagaa attacggtgc ataaagctgt agtagctttc cggatgctct
    22621 ttcggcagac cgacatattc gtccatgttg aaggtgacaa cgtgcttaaa gctgacctgg
    22681 cctgctttat gcatttcgac taacgcttta taggtggtca tcggcgtgcc gccagtcggc
    22741 aggcccagta caaacggacg atcggcagtc ggtttgaacg cattgatacg attgacgata
    22801 tggcgagcag cccatttgcc gacctgttca gcggtagtca gggggatcag tctcattatt
    22861 cacctcaata agtaaaatgt aagccgttgg cggattaggc atctttaagc gtaacctgga
    22921 tttgcgcaga caggcgtcaa tccgacctga ttttttgaat gataaaataa gttttctggt
    22981 ttagccagta aaagggagtg atggtaacga tatttggtga caaaactcac aaaagacacg
    23041 cgtttaattt gcgatacgaa ttaaattttc acacactctg tagcagatga tctaacaatc
    23101 tgattacaga acatcggcag tacaatttgc agcaaaataa aaatacggct ttaaacgagc
    23161 caaatagggt tctcgtaggg ggaataagat gaatatttta ggttttttcc agcgactcgg
    23221 tagggcgtta cagctcccta tcgcggtgct gccggtggcg gcactgttgc tgcgattcgg
    23281 tcagccagat ttacttaacg ttgcgtttat tgcccaggcg ggcggtgcga tttttgataa
    23341 cctcgcatta atcttcgcca tcggtgtggc atccagctgg tcgaaagaca gcgcaggtgc
    23401 ggcggcgctg gcgggtgcgg taggttactt tgtgttaacc aaagcgatgg tgaccatcaa
    23461 cccagaaatt aacatgggtg tactggcggg tatcattacc ggtctggttg gtggcgcagc
    23521 ctataaccgt tggtccgata ttaaactgcc ggacttcctg agcttcttcg gcggcaaacg
    23581 ctttgtgccg atcgccaccg gcttcttctg tctggtgctg gcggccattt ttggttacgt
    23641 ctggccgccg gtacagcacg ctatccatgc aggcggcgag tggatcgttt ctgcgggcgc
    23701 gctgggttcc ggtatctttg gtttcatcaa ccgtctgctg atcccaaccg gtctgcatca
    23761 ggtgctgaac accatcgcct ggttccagat tggtgaattc accaacgcgg cgggtacggt
    23821 tttccacggc gacatcaacc gtttctacgc tggtgacggc accgcgggga tgttcatgtc
    23881 cggcttcttc ccgatcatga tgttcggtct gccgggtgcg gcgctggcga tgtacttcgc
    23941 agcaccgaaa gagcgtcgtc cgatggttgg cgggatgctg ctttctgttg ctgttactgc
    24001 gttcctgacc ggtgtgactg agccgctgga attcctgttc atgttccttg ctccgctgct
    24061 gtacctcctg cacgcactgc tgaccggtat cagcctgttt gtggcaacgc tgctgggtat
    24121 ccatgccggc ttctctttct ctgcgggggc tatcgactac gcgttgatgt ataacctgcc
    24181 ggccgccagc cagaacgtct ggatgctgct ggttatgggt gttgtcttct tcgctatcta
    24241 cttcgtggtg ttcagtttgg ttatccgcat gttcaacctg aaaacgccgg gtcgtgaaga
    24301 taaagaagac gagatcgtta ctgaagaagc caacagcaac actgaagaag gcctcaatca
    24361 actggcgacc aactatattg ctgcggttgg cggcactgac aacctgaaag caattgacgc
    24421 ctgtatcacc cgtctgcgcc ttacagtggc tgactctgcc cgcgttaacg atacgatgtg
    24481 taaacgtctg ggtgcttctg gtgtagtaaa actgaacaaa cagactattc aggtgattgt
    24541 tggcgcgaaa gcagaatcca tcggcgatgc gatgaagaaa gtcgttgccc gtggcccggt
    24601 agcagctgcc tctgctgaag caactccggc aactgccgct cctgtagcaa aaccgcaggc
    24661 tgtaccaaac gcggtatcta tcgcggagct ggtatcgccg attaccggtg atgtcgtggc
    24721 actggatcag gttcctgacg aagcattcgc cagcaaagcg gtgggtgacg gtgtggcggt
    24781 gaaaccgaca gataaaatcg tcgtatcacc agccgcaggg acaatcgtga aaatcttcaa
    24841 caccaaccac gcgttctgtc tggaaaccga aaaaggcgcg gagatcgtcg tccatatggg
    24901 tatcgacacc gtagcgctgg aaggtaaagg ctttaaacgt ctggtggaag agggcgcgca
    24961 ggtaagcgca gggcaaccga ttctggaaat ggatctggat tacctgaacg ctaacgcccg
    25021 ctcgatgatt agtccggtgg tttgcagcaa tatcgacgat ttcagtggct tgatcattaa
    25081 agctcagggc catgtcgtgg cgggtcaaac gccgctgtat gaaatcaaaa agtaatctgc
    25141 tttatgcctg atgcgacgct tgacgcgtct tatcaggcct acaaaagctc agtccaggtt
    25201 ggataagacg cttaagcgtc gcatccaaca atgacgcagg actggcaaaa gcggtggaga
    25261 tcttctctgc cgcttttttt ttcatcaatc atccccataa tctcagtgag attttcgatt
    25321 ttcactaact aacagttgtc agcctgtccc gcttataaga tcatacgccg ttatacgttg
    25381 tttacgcttt gaggaatcca cgatgagtga ggcagaagcc cgcccgacta actttattcg
    25441 tcagatcatc gatgaagatc tggccagtgg taagcacacc acagtacaca cccgtttccc
    25501 gccggagccg aatggctatc tgcatatcgg tcatgcgaaa tctatctgcc tgaacttcgg
    25561 gatcgcccag gactataaag gccagtgcaa cctgcgtttc gacgacacta acccggtaaa
    25621 agaagatatc gagtatgtgg agtcgatcaa aaacgacgta gagtggttag gttttcactg
    25681 gtctggtaac gtccgttact cctccgatta ttttgatcag ctccacgcct atgcgatcga
    25741 actgatcaat aaaggcctgg cgtacgttga tgaactgacg ccggaacaga tccgcgaata
    25801 ccgtggcacc ctgacacagc cgggtaaaaa cagcccgtac cgcgaccgta gcgttgaaga
    25861 gaacctggcg ctgttcgaga aaatgcgtac cggcggtttt gaagaaggta aagcctgcct
    25921 gcgtgcgaaa atcgacatgg cttcaccgtt tatcgtaatg cgcgatccgg tgctgtaccg
    25981 cattaaattt gctgaacacc accagactgg caacaagtgg tgcatctacc cgatgtacga
    26041 cttcacccac tgcatcagcg atgccctgga aggtattacg cactctctgt gtacgcttga
    26101 gttccaggac aaccgtcgtc tgtatgactg ggtgctggac aacatcacca ttcctgttca
    26161 cccgcgccag tacgaattct cgcgcctgaa tctggaatac actgtgatgt ccaagcgtaa
    26221 gctgaacctg ctggtgaccg acaagcacgt tgaaggctgg gatgacccgc gtatgccgac
    26281 catttccggt ctgcgtcgtc gtggttacac tgcggcttct attcgtgagt tctgcaaacg
    26341 aatcggcgtg accaagcagg acaacaccat tgagatggcg tcgctggaat cctgcatccg
    26401 tgaagatctc aacgaaaatg cgccgcgcgc aatggcggtt atcgatccgg tgaaactggt
    26461 tatcgaaaac tatcagggcg aaggcgaaat ggttaccatg ccgaaccatc cgaacaaacc
    26521 ggaaatgggc agccgtcagg tgccgtttag cggtgagatt tggatcgatc gcgccgattt
    26581 ccgcgaagaa gctaacaagc agtacaaacg tctggtgctc ggtaaagaag tgcgtctgcg
    26641 taatgcttat gtgattaagg cagaacgcgt cgagaaagat gccgaaggta atatcaccac
    26701 catcttctgt acttatgacg ccgacacctt aagcaaagat ccggcagatg gtcgtaaagt
    26761 caaaggtgtt attcactggg tgagcgcggc acatgcgctg ccggttgaaa tccgtttgta
    26821 tgaccgtctg ttcagcgtgc cgaacccagg tgctgcggat gatttcctgt cggtgattaa
    26881 cccggaatcg ctggtgatca aacagggctt tgctgaaccg tcgctgaaag atgcggtagc
    26941 gggtaaagca ttccagtttg agcgtgaagg ttacttctgc ctcgatagcc gccattctac
    27001 ggcggaaaaa ccggtattta accgcaccgt tgggctgcgt gatacctggg cgaaagtagg
    27061 cgagtaattt taagtttcgc tatgccggat ggggcgttta cgtcgcatcc ggcaaggaac
    27121 agacaaacag tttcaaacgc taaattgcct gatgcgctac gcttatcagg cctacatgat
    27181 ctctgcaata tattgagttt gcgtgctttt gtaggccgga taaggcgttc acgc
//
LOCUS       DAZFFG010000063        27180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFFG010000063 DAZFFG010000000
VERSION     DAZFFG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.063"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..486
                     /locus_tag="ACHJ7J_002907"
     CDS             <1..486
                     /locus_tag="ACHJ7J_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO5001111.1"
                     /translation="DDTEVTGQEIWQEVQSGKWGSAGAYTPDADALNSAMTIKLAEIN
                     QWRDSRESAGLIFEWQAHRWDGGKVSHDRLSPVIAVAQCGELPEGFFWTDADNQDVRL
                     TATELMELESAMLQAMVVHGFRIHERQRQMKEDIGRLETPGEVRGYVVGWGTETLEAA
                     D"
     gene            483..863
                     /locus_tag="ACHJ7J_002908"
     CDS             483..863
                     /locus_tag="ACHJ7J_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HFO5001112.1"
                     /translation="MSKFTTPAVLEMLDDYRWRLVEPFEFWLTDNPDDVIYVPEGYVT
                     DLASVPRLLWALFPPHGRYAKAAIIHDWLYDNGLRTKVEADRIFLDAMTVLGVPRWRR
                     RLMYFAVRLFGKGKYSDTQQGTCC"
     gene            2361..2611
                     /locus_tag="ACHJ7J_002909"
                     /pseudo
     CDS             2361..2611
                     /locus_tag="ACHJ7J_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701867.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(2822..3184)
                     /gene="ssrA"
                     /locus_tag="ACHJ7J_002910"
     tmRNA           complement(2822..3184)
                     /gene="ssrA"
                     /locus_tag="ACHJ7J_002910"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3399..3881)
                     /gene="smpB"
                     /locus_tag="ACHJ7J_002911"
     CDS             complement(3399..3881)
                     /gene="smpB"
                     /locus_tag="ACHJ7J_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO5001113.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            4052..4489
                     /gene="ratA"
                     /locus_tag="ACHJ7J_002912"
     CDS             4052..4489
                     /gene="ratA"
                     /locus_tag="ACHJ7J_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO5001114.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            4479..4769
                     /gene="yfjF"
                     /locus_tag="ACHJ7J_002913"
     CDS             4479..4769
                     /gene="yfjF"
                     /locus_tag="ACHJ7J_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO5001115.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(4831..5172)
                     /gene="bamE"
                     /locus_tag="ACHJ7J_002914"
     CDS             complement(4831..5172)
                     /gene="bamE"
                     /locus_tag="ACHJ7J_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO5001116.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(5321..6982)
                     /gene="recN"
                     /locus_tag="ACHJ7J_002915"
     CDS             complement(5321..6982)
                     /gene="recN"
                     /locus_tag="ACHJ7J_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO5001117.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEVDMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(7068..7946)
                     /gene="nadK"
                     /locus_tag="ACHJ7J_002916"
     CDS             complement(7068..7946)
                     /gene="nadK"
                     /locus_tag="ACHJ7J_002916"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO5001118.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNALQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8069..8662
                     /gene="grpE"
                     /locus_tag="ACHJ7J_002917"
     CDS             8069..8662
                     /gene="grpE"
                     /locus_tag="ACHJ7J_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO5001119.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPDASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(8717..10003)
                     /gene="yfjD"
                     /locus_tag="ACHJ7J_002918"
     CDS             complement(8717..10003)
                     /gene="yfjD"
                     /locus_tag="ACHJ7J_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO5001120.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(10025..10816)
                     /gene="ypjD"
                     /locus_tag="ACHJ7J_002919"
     CDS             complement(10025..10816)
                     /gene="ypjD"
                     /locus_tag="ACHJ7J_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO5001121.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            10983..12344
                     /gene="ffh"
                     /locus_tag="ACHJ7J_002920"
     CDS             10983..12344
                     /gene="ffh"
                     /locus_tag="ACHJ7J_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO5001122.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            12481..12729
                     /gene="rpsP"
                     /locus_tag="ACHJ7J_002921"
     CDS             12481..12729
                     /gene="rpsP"
                     /locus_tag="ACHJ7J_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO5001123.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            12748..13296
                     /gene="rimM"
                     /locus_tag="ACHJ7J_002922"
     CDS             12748..13296
                     /gene="rimM"
                     /locus_tag="ACHJ7J_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO5001124.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            13327..14094
                     /gene="trmD"
                     /locus_tag="ACHJ7J_002923"
     CDS             13327..14094
                     /gene="trmD"
                     /locus_tag="ACHJ7J_002923"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO5001125.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            14136..14483
                     /gene="rplS"
                     /locus_tag="ACHJ7J_002924"
     CDS             14136..14483
                     /gene="rplS"
                     /locus_tag="ACHJ7J_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO5001126.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(14560..15042)
                     /gene="yfiB"
                     /locus_tag="ACHJ7J_002925"
     CDS             complement(14560..15042)
                     /gene="yfiB"
                     /locus_tag="ACHJ7J_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5001127.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(15058..16284)
                     /gene="dgcN"
                     /locus_tag="ACHJ7J_002926"
     CDS             complement(15058..16284)
                     /gene="dgcN"
                     /locus_tag="ACHJ7J_002926"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO5001128.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(16274..16792)
                     /gene="yfiR"
                     /locus_tag="ACHJ7J_002927"
     CDS             complement(16274..16792)
                     /gene="yfiR"
                     /locus_tag="ACHJ7J_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO5001129.1"
                     /translation="MRFSHRLFLLLIILLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            complement(16942..17307)
                     /gene="yfiL"
                     /locus_tag="ACHJ7J_002928"
     CDS             complement(16942..17307)
                     /gene="yfiL"
                     /locus_tag="ACHJ7J_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO5001130.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            17517..18587
                     /gene="aroF"
                     /locus_tag="ACHJ7J_002929"
     CDS             17517..18587
                     /gene="aroF"
                     /locus_tag="ACHJ7J_002929"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO5001131.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            18598..19719
                     /gene="tyrA"
                     /locus_tag="ACHJ7J_002930"
     CDS             18598..19719
                     /gene="tyrA"
                     /locus_tag="ACHJ7J_002930"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO5001132.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(19762..20922)
                     /gene="pheA"
                     /locus_tag="ACHJ7J_002931"
     CDS             complement(19762..20922)
                     /gene="pheA"
                     /locus_tag="ACHJ7J_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO5001133.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     misc_feature    complement(20944..21069)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(21021..21068)
                     /gene="pheL"
                     /locus_tag="ACHJ7J_002932"
     CDS             complement(21021..21068)
                     /gene="pheL"
                     /locus_tag="ACHJ7J_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO5001134.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            complement(21172..21513)
                     /gene="raiA"
                     /locus_tag="ACHJ7J_002933"
     CDS             complement(21172..21513)
                     /gene="raiA"
                     /locus_tag="ACHJ7J_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO5001135.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            complement(21784..22521)
                     /gene="bamD"
                     /locus_tag="ACHJ7J_002934"
     CDS             complement(21784..22521)
                     /gene="bamD"
                     /locus_tag="ACHJ7J_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO5001136.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            22656..23636
                     /gene="rluD"
                     /locus_tag="ACHJ7J_002935"
     CDS             22656..23636
                     /gene="rluD"
                     /locus_tag="ACHJ7J_002935"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO5001137.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEAMRADFEEHKDEVDWL"
     gene            23633..24364
                     /gene="yfiH"
                     /locus_tag="ACHJ7J_002936"
     CDS             23633..24364
                     /gene="yfiH"
                     /locus_tag="ACHJ7J_002936"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO5001138.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLALQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            24494..27067
                     /gene="clpB"
                     /locus_tag="ACHJ7J_002937"
     CDS             24494..27067
                     /gene="clpB"
                     /locus_tag="ACHJ7J_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO5001139.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         6801 a         6240 c         7139 g         7000 t
ORIGIN      
        1 gatgatacgg aggtaacggg tcaggaaatc tggcaggagg tgcagagtgg taaatgggga
       61 agtgccggcg cgtatacgcc ggatgcagat gccctgaatt ccgccatgac cattaaactg
      121 gcagaaatta atcagtggcg ggattcccgg gaaagtgccg gtttaatctt tgaatggcaa
      181 gctcatcgct gggatggtgg aaaagtgtct catgatcgtc tgtccccggt tattgccgtg
      241 gcacagtgcg gtgaattacc ggagggtttt ttctggacgg atgcggataa ccaggatgtc
      301 cggcttacgg caactgaact gatggaactt gagtcggcca tgttacaggc aatggttgtg
      361 catgggttca ggatccatga gcgacagcga cagatgaaag aagatatcgg gcgtctggaa
      421 acaccgggtg aagtccgtgg ttatgtggtt ggctggggaa ctgaaacgtt ggaggccgct
      481 gattgagtaa attcacgaca ccggcggttc tggagatgct tgacgattac cgctggcggc
      541 tggttgagcc gtttgaattc tggctgacgg ataacccgga tgatgtgatt tatgttccgg
      601 agggatatgt gacggacctt gcgagtgtgc cccggcttct gtgggcactg ttcccgccgc
      661 atggtcgtta tgcgaaagcc gcgattattc atgactggct gtatgacaat ggcctgcgca
      721 cgaaggtgga agctgacagg atatttctgg atgcgatgac ggtgcttgga gttccccgct
      781 ggcgcagacg gttgatgtat tttgcggtaa ggttgttcgg gaaagggaaa tacagcgaca
      841 cgcaacaggg gacctgttgc tgaaaaaaag cctgccatac agatgtatgg caggaaaggt
      901 atccaaggga gaagacactg cagtgcagta tcccttatgg tcggcggtga ttatataacc
      961 acagaacggc acatcaaaca gttttgtata tcgtttgtta aatttgtgta ttacatcaaa
     1021 agcaaggtag caacatgaat cataaatagg catagaatgt taacatgatg gtatgtgtta
     1081 ctgagcagta ccacgtattc acctgtgcct gtagcgagtc gcgacagagt aatactgcta
     1141 aaaaaagcct gccatatata tggcaggcaa aagtcagtta catataaaag aacaaccttg
     1201 aatgccaggg ttgtgtccct cattcatagt cgttaatgat tatgtataga tatggaaaaa
     1261 aataaagatg ttttgtttat ttattgttaa gtttgtgtgt tgcatctcag aattaataca
     1321 cggtgtcatc tgttatcatg ctaaataatg gataagcgaa attatattca tagttaatta
     1381 tattcattat tgcttttaac aaaagtaaat atcctccggc atagccggag gtttttcaaa
     1441 tgcgcctgta aggctctctt accagccgcg ccctaacagg cgcacacgat ctgacatttg
     1501 catccaactt cgttacttac ggcccgtagt ttgacacttg cagttgccag accgcaaggt
     1561 gttttaacaa atcaaaaggg gttttaataa ctggctcaaa gctgaaagct ttccggaacc
     1621 cccagcctgg ctggggtgct gtgcacaaaa aaagcccgca gtatctgcgg gctaagtatt
     1681 aatatattag tatatattag tatattgacg gatgcgttgt ttatactaat gcacggaact
     1741 gatgataact ctgatggcaa tcacaaaaaa taacagtacc ggtaagatat aacatggtgt
     1801 gttgtaatat ttttcccatt tctgccagaa cttgtttgtt tcatctcgtg atacttgttt
     1861 gcagtataaa agaaacctgc catatggcag gttaattaac aatggtcaaa cgggtttgta
     1921 ggtgtttcat atatattatt tttaatctta tatacgagta tttcgtgaaa tggaagtact
     1981 gtttttaatg aacggtaatt tataggattt ttattatata aagattctta tataaatgat
     2041 attatcacgc aaaaaacata aaaaaatccc gttcacatat gaacgggata aaattggcct
     2101 caatgctaca tgtttattac ttctgctcgt tattatgata tatgaatatg atatggatgt
     2161 aaaaaaaaat ctgaatttta ggatttctta tataaatatt atctttaatt atgccagata
     2221 aaagtcacta ctatatgaaa taagcaattc ttctacataa gggaatgata tgttgttgaa
     2281 agtttttatg ctccggagtg tataacagga gaaactggcg ttcatgttga gctgaactgt
     2341 cgtaactaaa atgctgtaca gactcactgg ccagccttta tccggtggta atgacattta
     2401 aatcagatac agcacgggac gttatgacat ggggcgacgt tgaagatcat caagtgaaac
     2461 tgtcgtgccg gagcttgagg aactggcagg agcaatggta caggcgcagg tcgatcgcaa
     2521 gacgagattt atcgtcgtca tcgggagatg aaggagggag ttgagtagtc tggaggattt
     2581 ggtttcaatt cgggcgtttg gcgttacgta agtggtaagt aagcatcatg ctgaattgtt
     2641 accattaatt acaaatagaa aaacctctga cttcacaatc agaggttttt ttgaaatgca
     2701 cgtgcattct gcgtgtatat ttttgtcgtt ttctggtcag aacacggtct ggtttgtgtc
     2761 ctgctgatac ttctaacttc ctgtttttac tggtattgtc cttacaccgt ccaatcatga
     2821 ttggtggagc tggcgggagt tgaacccgcg tccgaaattc ctacatcctc ggtactacat
     2881 gcttagtcag tctttacatt cgcttgccag ctgcggacgg acacgccact aacaaactag
     2941 cctgattaag ttttaacgct tcaaccccag gcagggcttc cacgcgatct cttttgggtt
     3001 tgacctctct tgatccccgt cctaagagcg gaggctaggg agagagggct ctaagcaggt
     3061 tattaagctg ctaaagcgta gttttcgtcg tttgcgacta ttttttgcgg ctttttacga
     3121 ggccaaccgc ccctcggcat gcaccttggg tttcgcaaat cccgtcgaat ccagaatcag
     3181 ccccaatgtg taaaggtaag tataccagat ttatgagcgc catgaccagc ctcaatggcg
     3241 ttatcgttaa agatttagcg accatgtagc ctgattttta ttcgatttag caatgggatg
     3301 gcaacatttt cgtcggatgt gatagccaat aagatgttca ttcgcgccgc cggagaggga
     3361 ggcgcggtga ggaactggtc aataattgga gtgcaggttt aacggtgagc gtttttcatg
     3421 atacgcgctt tatccacctg ccattcgcgt tctttgatat ctgaacgttt atcgtgctgt
     3481 ttcttacctt tggcgacgcc gattttcact ttgcaccagg catttttcca gtacagggag
     3541 agcgccacta cggtatagcc ttctcgattg acgcgaccgt acaatgagtc cagttcgcgc
     3601 tggttgagaa gtaacttgcg ggtacgggta ggatcgcaca ccacatgcgt ggaggccacg
     3661 gccattggcg tgatgttagc gccaaacaga aatgcctctc cgtcacgcag aaggacgtag
     3721 ctgtcgctga tattggcttt tcctgcgcgc agggatttaa cctcccagcc ttgcagggca
     3781 agtcccgctt cgaactcttc ttcgataaag tattcgtgac gggcgcgctt gttaagcgcg
     3841 atggtcgctg aaccaggttt atgtgctttt ttcttcgtca taagcgtcgt gaatcatcgg
     3901 taatctgaaa tcgaaaacac cccatatcaa tcctgcgggg gacaaggctc tatcttagca
     3961 tgaacccgat gttacccagc gccgggatag cgtttttttt acagcaggat aaatgatatt
     4021 atttgttgga tttttgttga tggaaattgt tatgcctcag attagccgga ccgcactggt
     4081 accctacagc gcggagcaaa tgtatcagtt agtgaatgac gttcagtctt atcctcagtt
     4141 tttgccgggt tgtaccggaa gtcggattct ggagtccact cctgggcaga tgactgcggc
     4201 ggtagatgtc tctaaggctg ggatcagcaa aacgtttact acccgcaacc agttgaccag
     4261 taaccaaagt attcttatga atctggtgga tgggccgttc aagaaattga ttggtggatg
     4321 gaagtttacg ccgctgagcc aggaggcgtg tcgtatcgag tttcatctcg actttgagtt
     4381 taccaataag ttgattgaac tcgcttttgg tcgcgtgttt aaagagctgg cgtctaatat
     4441 ggtccaggct tttacggttc gtgcgaaaga ggtttacagt gccaggtaaa attgccgttg
     4501 aggtggctta tgcgctacct gagaagcagt acctgcagcg cgtgacgctg caggagggcg
     4561 cgacggttga agaagctatt cgcgccagtg gcttgctgga attgcgtacc gatatcgatt
     4621 taactaaaaa caaagtcggc atttacagcc gtccggcaaa actaagcgat agtgtgcatg
     4681 atggcgatcg ggtggagatt tatcgccctc tcattgccga tccgaaagag cttcgcaggc
     4741 aacgagcaga aaaatcagcg aataaataac agacagaaaa aaggtgctca ttgagcacct
     4801 tttttaacgt ctttgagagc aactttatta ttagttacca ctcagcgcag gtttgttatc
     4861 aatattggtc aacacaccgc tactgttaaa ggttagcgtc agcgtttgct gcgttacacc
     4921 ttcatgacct ggttgctggc ggaagacata gaaccaggta ttcgtaccaa atggatcgga
     4981 catcagcggt gtacccaatg cgtacgcaac ttgttgttgc gtcatgccaa cacgtatttt
     5041 ggatacgtcg ttagcggtca gatagttccc ctggttgatg tcaggacggt aaaccactcg
     5101 ctccagagtg gaacagcctg cggtcaacat caatagtact gctgctgcag cagtcagcgt
     5161 tttacagcgc atagtgattt gattcctttt cgggcccgag cagtacgtgg ctcatctgta
     5221 atatgccgat gataatagac ctttcatcac tttaaaacct tttgctttcc ggtcttacgg
     5281 cgttttgctg tttactctga ccgtgaagca ggaaaaaagt ttacgccgca agcagttctt
     5341 tcgcattcgc cagtgtgtta cgtgtgactt cactgccacc aagcaggcgc gccagctctt
     5401 gtaaccgcgc ttttttatcc agagattgca tatgcgtttc tgtcatcgca ccatcggttt
     5461 ctttgctgac aaaatagtgt tgatgaccac atcccgcgac ttgtggcagg tgggtaacac
     5521 acatcacctg agttgattcg ccgagttgac gcagcagttt gccgacaact gccgctgttg
     5581 gaccgctaat ccctacatcc acttcatcaa aaatcagtgc cggggtttcc attttacgcg
     5641 ccgtgatgac ctggattgcc agtgcgatgc gggacaattc accaccggat gcgactttgg
     5701 caataggctg cattggctga cctgggttgg tggttacccg aaactcaata cgatcggcac
     5761 cgtcagcgcc caggtgatgc tcgtcaaatt taacatcgat cgtaaactgc ccatgcggca
     5821 ttgagagcgc atgcatactg tcggtgatca gctgtgcaag ttcttctgca tattgctggc
     5881 gttgttggtg taatgcgcgc gcggtttcca gtgcctgctg atgatgtttc gttaccgcca
     5941 gcgcgagcgt ttcttgtgag tcggcctgat cgtccagttg ctgctgttct tccagtagcg
     6001 actggtaata ctgtggtaat gcctcagggc tgacgtgatg tttacgtgcc aacgaaatct
     6061 gttttgagat gcgttgttca agttcaaaca gccggttggg atcgagatcc aaacgatcgc
     6121 agtagtggcg cagttcgtcg ctggcttcag caatctggat ggtagcttct tccagcatat
     6181 caagtacgcc ggacagtttg ctgtccatgc caatcaattc gctcaccagt tgtttagccg
     6241 tgtaaagctg actttgcagg tttgcgtctt caccgtcggc cattaatgcc aatgcattct
     6301 ggctggtggt cagcaattga ccgctgttcg ccagacgttt gtactcttcg tcgatttgct
     6361 caaactctcc gggctgcgga ttaaattcgt taagttcttt taattggtat tgcagcagtt
     6421 ccgcacgggc ggcgcgttcc tgacttaact gttgatgatg cgcgaggtca cggcagcttt
     6481 gatgccacaa ctgataacgt gcggtcattt cctgaagtag agaggtttca ttggcatagc
     6541 catcaagcag gaatttttgg tgctcaggtt tggtgagtaa ttgatgagcg tgctgaccat
     6601 ggatctgaat cagcaactga cccagttcgc gcagttgtga cagaggaaca gctgtaccgt
     6661 tgatgaaacc acgggagcga ccatcgctgc tgatcacgcg acgaagcaaa cattcatgcc
     6721 cgtcttcaag ctggttttct tccagccagc gcagagccgc tggcgtatct ttcagagaaa
     6781 aacgggcgca caggtcagcg cgagcagcgc cggtacgcac catgtcgact tcagcgcgac
     6841 caccgagaca aagaccgagg gcatctattg caatagattt acccgcgccg gtctcgccag
     6901 ttattacggt catgccgcta tgaaaatcaa tctcaagctc acgaacgata gcaaagttgc
     6961 tgatggtcag ttgtgccaac atagtcgttt tcctgtatga aaaaccatta ctgttattgc
     7021 gtacagtata aactggtttt atatacagta aagaggctgg cgtaaaatta gaataatttt
     7081 tttgaccagc cgagcttggt gcttaatgta ttgaaataac tgtaatcttt cggatgaatc
     7141 agattcagat ggtaatcaca gcgacgaatc aggacatctt caccttcctg aatcggcagt
     7201 gctatctggc tgtcgcaact gatttccagg tcgttacggc gatgcgaaaa acgcagacgg
     7261 atcgtgctgc tgctgtttat gaccagtggt cgtgctgaca acgtatgcgg gaacatgggc
     7321 accagggtaa tcgcatccag agagggggtc agaataggac cgcccgcaga gagggaatag
     7381 gcggtggagc ctgttggcgt cgaaataatt agcccatcag atcgctgaga aaacgcaaag
     7441 atctcgtcga tatacacttc gaactctatc atatgcgcca ctttgcctgg atgaagtacc
     7501 acttcattta tcgcggtgct gatgcgcttt tggcaatctt gctgacagac ttgcgcttcc
     7561 agcaaaaaac gtttctcgct gatgtagtga ccttccagca catcggctaa ttgttgcagg
     7621 gcgttatcgg ggtcaaggtc agtcaggaaa cccaggttgc cacggttgat tccaataact
     7681 ttaatatcgt agcgggcgag cgtgcgcgcc gcgcccagca tattaccgtc gccaccaacg
     7741 actaccgcga gatcagccag ttgcccaatc tccgcgagcg tgccagtttt cacattcttc
     7801 agttgcaatt cgtgagcgat ttgttgctca acgatgacct cgtaaccttt tgtgcacagc
     7861 cagcggtaga gcatttcatg tgttgtcagt gcagtagggt gccgtgggtg tcccacaatg
     7921 ccaatacact tgaaatgatt attcattttt ccgaggtcct tgttgcgaag attgatgaca
     7981 atgtgagtgc ttcccttgaa accctgaaac tgatccccat aataagcgaa gttagcgaga
     8041 tgaatgcgaa aaaaacgcgg agaaattcat gagtagtaaa gaacagaaaa cgcctgaggg
     8101 gcaagccccg gaagaaatta tcatggatca gcacgaagag attgaggcag ttgagccaga
     8161 cgcttctgct gagcaggtgg atccgcgcga tgaaaaaatt gcgaatctcg aagctcagct
     8221 ggctgaagcc cagacccgtg aacgtgacgg cattttgcgt gtaaaagccg aaatggaaaa
     8281 cctgcgtcgt cgtactgaac tggatattga aaaagcccac aaattcgcgc tggagaaatt
     8341 catcaacgaa ttgctgccgg tgattgatag cctggatcgt gcgctggaag tggctgataa
     8401 agctaacccg gatatgtctg cgatggttga aggcattgag ctgacgctga agtcgatgct
     8461 ggatgttgtg cgtaagtttg gcgttgaagt gatcgccgaa actaacgtcc cactggaccc
     8521 gaatgtgcat caggccatcg caatggtgga atctgatgac gttgcgccag gtaacgtact
     8581 cggcattatg cagaagggtt atacgctgaa tggtcgtacg attcgtgcgg cgatggttac
     8641 tgtagcgaaa gcaaaagctt aatttctgct ttcgtaataa ttcacggccc tgcatgcgaa
     8701 tgccgggccg ttttcgttac tccgccacac tctcgcgcag cggtttcaca ggaaaaactt
     8761 ttacctgctt aatcatattg tcctgtacgt cgagaatatc gatatcgtac tcgccaatac
     8821 gcacgcgggt gcctgcgaca gggatctctt ccagtgcctc aagaatgacg ccattaaccg
     8881 tgcgggcatc atcttccggt agatgccagt taaaggcttt gttgatttcc cgcacgttgg
     8941 cagtgccatc aataatcacc gaaccgtcgt tttgcggcgt gacttcttcg gcaagtgttg
     9001 gcgacatcga cgtagtgaaa tcgccgacaa tctcttccag aatatcttca accgtcacca
     9061 gcccctgaat gtctccatac tcgttgacga ccaggccgac tttctttttg ttgcgctgaa
     9121 actttaccaa ctgcgtgctg agcggcgtac cttccggcac aaaatagatc tcgtccgcgg
     9181 cgcgcagcat ggtttctttg gtgaactctt ttttctccga catcaaccgc caggcttcac
     9241 gtactcgcag catactgatg gcgtcgtcca gcgaatcacg gtagagcacg atgcgcccgt
     9301 gaggtgagtg ggagagttgg cgcagaatcg atttccagtc atcgttgata tcaataccga
     9361 taatttcact gcgcggcacc atgatgtcat caacggtcat tttttccaga tcgagcaccg
     9421 acagcagcat atcctgattg cgacgggaaa tttgtgagcg cgattcgtgc acgatagtgc
     9481 gcaactcttc tttgctcaaa gagccgctaa ccacgatatc ggttttgata cccatcatgc
     9541 gcatcagcat acgggtgata gcattcagca accagaccag cggcatcatc aaaatttgca
     9601 gcggagccag cagaaaacta ctcggataag cgactttttc cgggtacagc gcggcaatgg
     9661 ttttcggcaa tacctcagca aaaaccagta cgacaaaagt cagcacacca gtcgcaattg
     9721 ccacgcccgc atcgccgtac aaacgcatcc caacaatagt gccgagcgcg gaggcaagaa
     9781 tattgaccag gttattgccg attaacacca ggcttatcag gcggtctggc ttacgcagca
     9841 atttttcgac gcgtttggcc gagcgattac cctgtttcgc catatgtcgc agacgatagc
     9901 ggttgagggt catcattccg gtttcggacc cggaaaaata ggctgaaatg accaccatga
     9961 tgatcagaat aatgatcaac gtagtagtag aaatgtgttc cagggggaaa ctccttttct
    10021 gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt gtcagaatga
    10081 ctgcacccgc aacgttaaac cagacgacgc gacgtccacg ccatccttca tgataatgtc
    10141 cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc ttgtcgatat
    10201 tttccatgct aaataagttg tgcatgtaga acaggccagt gcaaagcgtg agcgttagca
    10261 gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc aatggcggca
    10321 tttcctggtt aaacgccagc ttcttgttct tcagttggta atcaatccac gccagttgca
    10381 gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag ccaatgtgca
    10441 ccagcatccc aggcgtagct tccagatggg tgatgtattc attgggcatg aaggtcgcca
    10501 gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag ccacgattgc
    10561 gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa ccaacgttca
    10621 gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc agagcgattg
    10681 cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag ccgccgtttt
    10741 tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca agcgcgagca
    10801 gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga agcagtatag
    10861 cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca tgttatactg
    10921 cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc aggcgagaga
    10981 caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc agtggccgtg
    11041 gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg gcgctgctgg
    11101 aggcggacgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa gagaaagcgg
    11161 ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa atcgtccgta
    11221 acgaactggt tgcggcgatg ggcgaagaga accagaccct gaatctggct gcgcaaccgc
    11281 ctgcggtcgt gctgatggca ggcctgcaag gtgccggtaa aacaaccagc gttggtaaac
    11341 tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct gctgacgtct
    11401 atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc gttgatttct
    11461 tcccttctga tgttggtcag aagccggttg atattgttaa cgcggcgctg aaagaagcca
    11521 aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac gttgacgaag
    11581 cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa accctgtttg
    11641 tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc aatgaagcgt
    11701 taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc ggtgcggcgc
    11761 tctctattcg tcacatcact ggcaaaccga tcaagttcct cggtgttggc gagaaaactg
    11821 aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc atgggcgacg
    11881 tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca gagaaattag
    11941 ccagcaagct gaaaaaaggt gacggcttcg atctcaacga cttccttgag caactgcgcc
    12001 agatgaaaaa tatgggcggc atggccagcc tgatgggcaa actgccgggc atggggcaga
    12061 tcccggataa cgtcaagtca cagatggacg ataaagtgct ggtgcgtatg gaagccatca
    12121 tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt tcgcgtaaac
    12181 gccgtattgc tgccggttgc ggtatgcagg tgcaggacgt taaccgtctt ctgaaacagt
    12241 tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg aagatgatga
    12301 gaagcatgaa ggggatgatg ccgccgggat ttcctggtcg ctaaatgacc ttatgcgaca
    12361 gagttattaa ctgctgattg cattttctcc agaaatcagt aaaattttcg ggcttttaat
    12421 atgacaccgg actccgttcc tcgatggggt ccggttgttt tattcacaca agaggatgtt
    12481 atggtaacta ttcgtttagc acgtcacggc gctaaaaagc gtccgttcta ccaggttgtt
    12541 gtcgctgaca gccgtaatgc acgcaacggt cgcttcatcg agcgcgttgg tttcttcaac
    12601 ccaatcgcta gcgaaaaaga agaaggcact cgcctggatc tggatcgcat cgctcactgg
    12661 gttggccagg gcgcaactat ttctgatcgc gttgctgcgc tgatcaaaga agtaaacaaa
    12721 gcagcttaat ctgtcacggt ggtcatgatg agcaaacaac tcaccgcgca agcacctgtt
    12781 gatcccatcg ttttgggaaa aatgggttcg tcttacggta ttcgtgggtg gctcagagtg
    12841 ttttcttcca ccgaagacgc cgaaagcatt tttgactatc agccctggtt tatccagaag
    12901 gcgggtcagt ggcagcaagt ccagctggaa agctggaagc accacaatca ggacatgatc
    12961 atcaagctga aaggcgttga cgatcgtgat gcggcgaacc tgctgacgaa ttgtgaaatt
    13021 gtcgtggatt catcgcagct gcctcagctt gaagagggtg actactactg gaaagacctg
    13081 atgggctgcc aggtagtaac cactgaaggc tacgatctcg gtaaagtcgt cgatatgatg
    13141 gaaaccggat ccaatgacgt tctcgtcatt aaggcaaacc tgaaagatgc gtttggtatc
    13201 aaggaacgtc tcgtaccgtt cctcgatggg caggttatca agaaagtcga tctcactact
    13261 cgttcaatcg aagtagattg ggatcctggt ttttaaacca ccggataaac ggtaaaagac
    13321 ggcgctatgt ggattggcat aattagcctg tttcctgaaa tgttccgcgc aattaccgat
    13381 tacggggtaa ctggccgggc agttaaaaat ggcctgctga gcatccagag ctggagtcct
    13441 cgcgatttca cgcatgaccg gcaccgtacc gtggacgatc gtccttacgg cggcgggccg
    13501 gggatgttaa tgatggtgca acccttgcgg gacgccattc atgcagcaaa agccgcggcg
    13561 ggtgaaggcg caaaggtgat ttatctttca ccacagggac gcaagcttga tcaagcgggc
    13621 gtcagcgaac tggcaacgaa tcaaaaattg attctggtgt gcggtcgcta cgaaggtata
    13681 gatgagcgcg tgatccaaac cgaaattgac gaagaatggt caatcggcga ttacgttctc
    13741 agtggtggtg agttaccagc aatgacgctg attgactccg tttcccggtt tattccggga
    13801 gtactgggac atgaagcctc ggcaacggaa gattcctttg ctgaaggatt gctggattgc
    13861 ccgcactata cgcggcctga ggtgttagaa gggatggaag ttccgccagt gttactgtcg
    13921 ggcaaccatg ccgagatacg tcgctggcgt ttgaaacagt cgctgggccg tacctggctt
    13981 agaagacctg aacttctgga aaacctggct ctgactgaag agcaagcaag gttgctggcg
    14041 gagttcaaaa cggaacacgc acaacagcaa cataaacatg atgggatggc gtaagccccc
    14101 gagatatcag tttacccagg ataagagatt aaattatgag caacattatt aagcaacttg
    14161 aacaagagca gatgaagcag gacgtacctt ccttccgtcc gggtgatacc gtggaagtga
    14221 aagtatgggt tgttgaaggt tccaaaaaac gtctgcaggc attcgagggc gtggttatcg
    14281 ctattcgtaa ccgcggtctg cactctgcat tcactgttcg taaaatttcc aacggcgaag
    14341 gcgttgagcg tgtcttccag actcactctc cggtagttga cagcatttct gtcaaacgtc
    14401 gtggtgctgt tcgtaaagct aaactgtact acctgcgtga gcgtactggt aaggctgctc
    14461 gtatcaaaga gcgtcttaac taagatttcg cttaagcgac atcctgttaa gaagggctgg
    14521 ccaattggct ggcccttttt ttatctgttt gctcgtgttt taaggggtag taatcaccac
    14581 tgcgacgcgg cggttctcgg cgcggccctg ggcggtcttg ttactggcta tgggatattt
    14641 ttttcctaaa ccctgtgtgg tgagattgct gcgtggaatt tgtccaccca tagcccatgc
    14701 atcggccacg acattcgccc gtttcaatga taagccttcg ttgtaactgt cttcaccata
    14761 gttatcggtg tgtccatcca tacgggcatg tgttagccct gtcgaggcca atttggctgc
    14821 catggtttgg atctgttgct ggctttccgg gagcaatttg tagtcatttt ttgcgaacag
    14881 aatggcatct gataagccca gcgaccagtc gccggcggat tcagtaaatc cataagattg
    14941 catagcggcg acttgctcag gagtaaactt tccctgaggg gactggcagc cagtcagtat
    15001 tagtgaggtg aaaatcaggg gtgctaccag gtgctttatc atatcgatat atccttgtta
    15061 tctcaccagc ttttcggcac gctggtgttt ggcctgatac atattgtgat cggccagctc
    15121 ttgtaatttt tccgcagaag cgtgcgcaat ggtcattgcg taaccaatgc ttaatgtcat
    15181 ggtggtctga tggccattat gaagatcaaa cgggcgatta aagatttgtg tcagtgctga
    15241 gcatatctgc tgcacttcag attccgactg tacatcatag agcaccatag cgaattcatc
    15301 gccgcccagg cggtatgctt tatgtcgcag cccgccaaat tcagctaacc gttttgcgat
    15361 ttcaatcaag actctatcgc ccgtcgcatg accccaggta tcattgatat atttgaaatt
    15421 atcgccatca agaaatagta acgccgacgt ttttcgggca tcggaattgt tcatcaacgt
    15481 attgatgccg ctacgaaacg ctgcgcggtt agccagcccg gttaatgggt catgtagcgc
    15541 ggtacgcaga agctgcgcat ttttggcctg taaacgaagc tgccactctt ccatttcatc
    15601 cagcagactg ttgaagtcga gagcgaagcg gtgaaactca gcgatacgtt cttccgaaac
    15661 tcgtcgggaa aaattgcggt tggaacgcac atcatgtacg acatcggtga tatttttcag
    15721 tgcttccacc aggccattgt gcaaatggcg ggtgagggta attgcgattc ctgatgccag
    15781 cagaatacaa ccggtcagta cggcgagcga aaaccagata aaatggctga ttgaactgtc
    15841 gcgagcggtt aagcgtactt cgccaatggt ttcgccattg tgacgaatcg gctgaatgat
    15901 gggggcaggg aagagccagt ggcttatgaa attgctgaaa gtatcgcctg gatccttacg
    15961 ggtgtaatgc caggatgcca gaatattttg ctgcttatca cgtacttctg cagttgaaaa
    16021 ttgcccttgc tggcccagcg ctgccagtgt ttcagttgct gcagggccat cggcaaaaac
    16081 gacagctgct tccagactgt aagtcattgt tgctgctgtc agtgccaggt ttttttgcgc
    16141 gtactgtttc agggtcagca ccgaggtcac ggaaagcagc aaccagatca gcatcatagt
    16201 gataaatatg ctggtcatac tgatgttgcg taatgctctt ttaaacgtgg ggcgcttatt
    16261 aagagaatta tcgttatcca tcatttttct tccgtgcgag cattaagaca tccgggttga
    16321 cctttacacc gctacgcgat aaggcatcca ggtttacggc aaatctgacg tcattattgt
    16381 ggatgatcag acaaaaggcg ctaccaataa tgcactcggt attttgttcg gcaattaata
    16441 ataacgcctt tgacggatat tgttcggtta attccatctg gaatgtcggt gactcgttgc
    16501 caaaataaaa accattgcag cctgaaatca tcgcctcttg ttgagtgtga atgatgacag
    16561 gcagataagg caaagatgtc gcggcattct cttgtagggc ggtactaaaa cgtgaagatg
    16621 aaaagataca taactttggt ggccctgata gcgcaggcca gcgggtgtaa ctgacaatac
    16681 cggaaaccat catacgcacg tttttcgcaa cgtcagaagg ctcctgagcg agtattggtg
    16741 ctcccgtcag caggataatc agcagtaaga acagtcggtg agaaaaacgc attcagggat
    16801 ccccaccatc ttcgctcgaa atgcatcgat attttttcag aatgctcatt aaaatatcat
    16861 tatttcatgc agtcgtcatc attgtgagaa ataaaacata atgagtaaaa tcttatgttt
    16921 aatttatttg tgggatttta attaatttaa tcgtattgtg ctggcatttt catcagcccc
    16981 tttttgccat acttcatgca gttggctggc attttcaaca ttgttgcagc tggcaggaaa
    17041 gcttttaccg gaaagtcctc gggcataagt aaaatcggtc tggcaagttt tcttttgtcc
    17101 ttcggcatat cctttaaggt atagaccgcg atccgcctgt gaatcgctaa atgcatcgtc
    17161 atcttttatg gcgctgcccg atatcgcatc ttccatacca acatcatacc agtcggtgct
    17221 ggtcaaggtt ggcgcgtgag tataaggatc aatctggcat ccgctaacca gtaaagccaa
    17281 caagggggcg ataaactttt tcatcatttc tttctccttt ttcaaagcat agcggattgt
    17341 ttttaaaggt tgtgtaaatt tatctataca gaagcaaagg tttaaagcgc gaataaattg
    17401 cttatgtaaa gaaaagtgta cgaaatatgg attgaaaact ttactttatg tgttatcgtt
    17461 acgtcatcct cgctgaggat caactatcgc aaacgagcat aaacaggatc gccatcatgc
    17521 aaaaagacgc gctgaataac gtacatatta ccgacgaaca ggttttaatg actccggcac
    17581 aactgaaggc cgcttttcca ttaagcctgc aacaagaagc ccagattgct gactcgcgta
    17641 aaaccatttc agatattatc gccgggcgcg atcctcgtct gctggtagta tgtggtcctt
    17701 gttccattca tgatccggaa actgctctgg aatatgctcg tcgatttaaa gcccttgccg
    17761 cagaggtcag cgatagcctc tatctggtaa tgcgcgtcta ttttgaaaaa ccccgtacca
    17821 ctgtcggctg gaaagggtta attaacgatc cccatatgga tggctctttt gatgtagaag
    17881 ccgggctgca gatcgcgcgt aaattgctgc ttgagctggt gaatatggga ctgccactgg
    17941 cgacggaagc gttagatccg aatagcccgc aatacctggg cgatctgttt agctggtcag
    18001 caattggtgc tcgtacaacg gaatcgcaaa ctcaccgtga aatggcctcc gggctttcca
    18061 tgccggttgg ttttaaaaac ggcaccgatg gcagtctggc aacggcaatt aacgctatgc
    18121 gcgccgccgc ccagccgcac cgttttgttg gcattaacca ggcagggcag gttgcgttgc
    18181 tacaaactca ggggaatccg gacgggcatg tgatcctgcg cggtggtaaa gcgccgaact
    18241 atagccctgc ggatgttgcg caatgtgaaa aagagatgga acaggcggga ctgcgcccgt
    18301 ctctgatggt agattgcagc cacggtaatt ccaataaaga ttatcgccgt cagcctgcgg
    18361 tggcagaatc cgtggttgct caaatcaaag atggcaatcg ctcaattatt ggtctgatga
    18421 tcgaaagtaa tatccacgag ggcaatcagt cttccgagca accgcgcagt gaaatgaaat
    18481 acggtgtatc cgtaaccgat gcctgcatta gctgggaaat gaccgatgcc ttgctgcgtg
    18541 aaattcatca ggatctgaac gggcagctga cggctcgcgt ggcttaagag gtttattatg
    18601 gttgctgaat tgaccgcatt acgcgatcaa attgatgaag tcgataaagc gctgctgaat
    18661 ttattagcga agcgtctgga actggttgct gaagtgggag aggtgaaaag ccgctttgga
    18721 ctgcctattt atgttccgga gcgagaggca tctatgttgg cctcgcgtcg tgcagaggcg
    18781 gaagctctgg gtgtaccgcc agatctgatt gaggatgttt tgcgtcgggt gatgcgtgaa
    18841 tcttactcca gtgaaaacga caaaggattt aaaacacttt gtcctgcgtt acgcccggta
    18901 gttatcgttg gcggcggcgg tcagatggga cgcctgttcg agaagatgct gacactatcg
    18961 ggttatcagg tgcggattct ggagcaacat gactgggatc gagcggctga tattgttgcc
    19021 gatgccggaa tggtgattgt tagtgtgcca atccacgtta ctgagcaagt tatcggcaaa
    19081 ttaccgcctt taccgaaaga ttgtattctg gttgatctgg catcagtgaa aaatggacca
    19141 ttacaggcca tgctggcggc gcacgatggc ccggtactgg ggttacaccc gatgttcggc
    19201 ccggacagcg gtagcctggc aaagcaagtt gtggtctggt gtgatggacg taagccggaa
    19261 gcataccaat ggtttctgga gcaaattcag gtctggggcg ctcggttgca tcgtattagc
    19321 gctgtcgagc acgatcagaa tatggcgttt attcaggcac tgcgccactt tgctactttt
    19381 gcttacgggc tgcacctggc agaagaaaat gttcagcttg agcaacttct ggcgctctct
    19441 tcgccgattt accgccttga gctggcgatg gtcgggcgac tgtttgctca ggatccgcag
    19501 ctttatgccg acatcattat gtcgtcagag cgtaatctgg cgttaatcaa acgttactat
    19561 aagcgtttcg gcgaggcgat tgagttgctg gagcagggcg ataagcaggc gtttattgac
    19621 agtttccgca aggtggagca ctggttcggc gattacgcac agcgttttca gagtgaaagc
    19681 cgcgtgttat tgcgtcaggc gaatgacaat cgccagtaat aatccagtgc cggatgattc
    19741 acatcatccg gcaccttttc atcaggttgg atcaacaggc actacgttct cacttgggta
    19801 acagcccaat accttcattg aacgggtgat ttcccctaac tctttcaatg ctttttgcat
    19861 ttccgctgat tcaagattgg cctgaatatc cagataaaac atctcttccc atggattacc
    19921 gtgaatcggg cgtgattcca gacgggtcat aatcagattg tggttgcgca gtaccagcaa
    19981 cgcttcaacc agcgcaccgg cttgttgccc ggtcgccatt aacaacgtcg ttttcgccgg
    20041 aacctgatca gacacgttaa tggctttacg cgccaacacc acaaatcggg tgaagttttg
    20101 tcgctgattc gcttcaatcc gctccagtac ctgcaaaccg tacaaagtgc cgccagcttc
    20161 gcttcccaac gcagcaacat gcggtgattt tgcctgtgca accttttcca ttgccgcaga
    20221 cgtactttcg gtatattcaa tcttccagtg cggataacga ttaaggaatt tgctgcattg
    20281 ctggaatggc tgcggatggc tgtagaccgt attgatggtg gataaatcag tcgtgccgga
    20341 gactaacaaa caatggtcga tagttaacgt catctcgcca acaatcgaca agctggtatg
    20401 ttgcagcaga tcgtaaacgt catttatggc accggagctg gtattttcaa tcggtacgac
    20461 ggcatagtcg gcctggccgg tttccacctg attaaaaata tcggcaaatt tggcgcagcc
    20521 actttcaatg aattgctcaa agtgacgcgc agcatactgg cgcgccgcaa gatgggaata
    20581 agaacctttg gggccgagaa aagcgatgcg tgctgagtgc ggattaattt tattgagatg
    20641 ttgttggagc aaagcctgct gagttaatac ggaatcttca atgatgagct ggaacaggcg
    20701 agtaatgtaa tgggcgtcca gatggtgcgc tttaccgagc gtaattaatc tttccagtaa
    20761 atcgcgttca cgatcaatat cacgtaccgg gcgatgcgag agcagtttgg cttttcccac
    20821 ctcgacggcc agttcgcgcc gctctgcgag taatgctaat aatttttcat ccagcgcgct
    20881 gattttctct cgcagcgcca gtaacgggtt ttccgatgtc atagtgttgc ctttttgtta
    20941 tcaataaaaa aggccccccg atttgggagg ccttattgtt cgtcttcgca ttctgtttca
    21001 cacgacgaaa cgcctcccat tcaggggaag gtaaaaaaga atgcgaagaa aaacggtgta
    21061 tgtttcatgt ttgtttcctt aagtgacttt agtacagtac ccgtactgtt ttcacgctgt
    21121 caacaaaaaa cgcgcccgaa ggcgcgttgg cgatacactc aatataaagg actactcttc
    21181 ttcaacttct tcgacgaagt tggcgtcttt caccgatgtt gcggcacgac gtgcttcgcc
    21241 tttgtgctgc agtttattga gctgccgttc cagcttgttg atcaattcgt taattgcggt
    21301 gtacatatct tcatgtttac cactggcaac cagaacgccg ttaggtgtat tgattgtggc
    21361 gtcagcaaca aacccttgtg gctctttgga cagaatgata tgtggattaa tcagatgtgt
    21421 ttgccatttt tccagtttgg cgagacggtc tgcgacatgt tggcggatgg ccggagtaat
    21481 ttccatttgt ttgctggtaa tgttcattgt cataaatttt acctcttgtc ttcccgtctt
    21541 ggtgaattca gcataccgtt cctgatgtca aaatgtgtga tggaaatctc ataattttgt
    21601 cactttttgt caacgaatct tttttgtgag aaatcgcagg aagaggtgtt tttttacttg
    21661 aggaatatct caaagcgagc catatttgat gagatcgata gtgactaaat cgcttcagtt
    21721 tcacaactga cagaataaaa aaacggcagc tcaagggctg ccgttttgtg tttcaggttt
    21781 ctgttatgta ttgctgctgt ttgcggcgat gattttcgct actttttcag cttgcgcatt
    21841 catctgcatc tgacggtatg cattttccat cagcggcagc gcatcacgcg tagcctgggt
    21901 atccgggtag tcgcgcaaca tgccttctac gcggttaacg acggcaaccc atgcgccacg
    21961 ttctgtatag tactcggcca cggagtattc atatttcgcc agacgatctt tcaggaatac
    22021 cagacgtttg gtggcatcgg tggtgtactg actgtttgga tagccgcgca ccagtttgga
    22081 aaagtcacta aacgcagctc gtgcatgttg aggatcgcga tcgctacggt caacgccaaa
    22141 gaacccttgc agcgcactgt catccagcgc catattggtc aggccacgca tgtacatgac
    22201 ataatcgata ttcggatggg tcgggttaag gcgaataaaa cgatcgatgg cagcctgcgc
    22261 taacggcaaa tcggcgtttt tatagtaggc gtagatgaga tccagctgca cctgctgcga
    22321 atacggacca aacggatagc gattatctaa cgcttccagt tgcgttattg cctgtctcca
    22381 gttaccgtcc tgcagctttt gttgtgcagt cgcgtaaatt tcatttggcg gattatcagg
    22441 tacttcttcc tttgaccccg agcaacccgc caaaaacagg cttagtgtgg cggctgccac
    22501 cagatatttc atgcgcgtca tgacgttttg actttcctca aaatgtaata cgggagattc
    22561 tctgttcctg ctcccggtta agaccagcta caatagcaca ctatattaaa cggcaaagcc
    22621 gtaaaacccc aacgataaac gaagaagcag tatatatggc acaacgagta cagctcactg
    22681 caacggtgtc cgaaaaccaa ctcggtcaac gcttagatca ggctttggcc gaaatgttcc
    22741 cggattattc acgttcgcga ataaaagaat ggatcctcga ccagcgcgtg ctggttaacg
    22801 gcaaactttg tgataagccg aaagaaaaag tattgggtgg cgagcaggtt gccatcaacg
    22861 cagagattga agaagaagcg cgttttgaac cgcaggatat cccgctggat atcgtctatg
    22921 aagatgaaga catcatcgtc attaataaac cgcgcgacct ggtggttcat cctggcgcgg
    22981 gtaacccgga tggcacggta ctgaatgcgt tgcttcatta ttatccgcct attgccgatg
    23041 taccgcgtgc gggcatcgtc catcgtctgg ataaagacac cactggcctg atggttgtgg
    23101 caaaaaccgt tccggctcag acgcgtttag tcgaatcttt gcaaaggcgt gaaattactc
    23161 gtgagtacga agcggtggcg attggtcata tgaccgctgg cggtacggtg gatgagccaa
    23221 tcagtcgcca cccgaccaaa cgtactcaca tggcggtgca tccgatgggc aaaccagcgg
    23281 tgactcacta tcgcatcatg gaacacttcc gtgtgcacac gcgtctgcgg ttgcgtctgg
    23341 aaactggacg tacgcaccag atccgcgtgc atatggccca tatcactcat ccgctggtgg
    23401 gcgatccggt ttatggtggc cgtccgcgtc cgccaaaagg tgcttcggaa gcgtttatct
    23461 ccacgctgcg taagtttgac cgccaggcgc ttcatgcaac catgctgcgt ctttatcatc
    23521 cgatctccgg catcgaaatg gaatggcatg cgcctattcc acaagatatg gtggagctga
    23581 ttgaggcgat gcgtgccgat ttcgaagaac ataaggatga agtggactgg ttatgagtaa
    23641 gctgattgtc ccgcagtggc cgctgccaaa aggtgttgcg gcttgtagct ctactcgtat
    23701 cggcggcgtg agcttgcccc cgtatgactc gctcaacctc ggtgcccatt gtggcgataa
    23761 cccggatcac gttgaggaga atcgcaagcg actttttgct gcgggcaatt tgccttctaa
    23821 accggtctgg cttgagcagg tacacggcaa agatgtgctt aagcttactg gcgaacctta
    23881 tgcctcaaaa cgagcagatg cctcttatag caatacgcct ggcacggttt gcgcagtgat
    23941 gactgccgac tgcctccctg tgctgttttg caatcgagcg ggaacggaag tcgccgccgc
    24001 tcatgctggc tggcgtggac tgtgcgcagg cgtgctggaa gagacagttt cctgttttgc
    24061 tgataatccg gaaaatattc tcgcctggtt agggccggca attggtccac gcgcgttcga
    24121 agtgggggcg gaggttcgcg aggcgtttat ggcagcagac gccaaagcga gtacagcttt
    24181 cattcagcat ggtgataagt atctggcgga tatttatctg cttgccctgc agcgtctggc
    24241 gagcgttggt gttgaacaaa ttttcggcgg cgaccgttgt acatatacgg aaaatgagac
    24301 tttcttctct tatcgtcgcg acaagaccac cgggcgtatg gcaagtttca tttggctgat
    24361 ataacctaaa gaatcaagac gatccggtac gcgtgatttt cttttcacat taatctggtc
    24421 aataaccttg aataattgag ggatgacctc atttaatctc cagtagcaac tttgatccgt
    24481 tatgggagga gttatgcgtc tggatcgtct tactaataaa ttccagcttg ctcttgccga
    24541 tgcccaatca cttgcactcg ggcacgacaa ccaattcatc gaaccacttc atttaatgag
    24601 cgccctgctg aatcaggaag ggggttcggt tagtcctttg ttaacatcag ctggcataaa
    24661 tgctggccag ttgcgcacag atatcaatca ggcattaaat cgtttaccgc aggttgaagg
    24721 tactggtggt gatgtccagc catcacagga tctggtgcgc gttcttaatc tttgcgacaa
    24781 gctggcgcaa aaacgtggtg ataactttat ctcgtcagaa ctgttcgttc tggcggcact
    24841 tgagtctcgc ggcacgctgg ccgacatcct gaaagcagca ggggcgacca ccgccaacat
    24901 tactcaagcg attgaacaaa tgcgtggagg tgaaagcgtg aacgatcaag gtgctgaaga
    24961 ccaacgtcag gctttgaaaa aatataccat cgaccttacc gaacgagccg aacagggcaa
    25021 actcgatccg gtgattggtc gtgatgaaga aattcgccgt accattcagg tgctgcaacg
    25081 tcgtactaaa aataacccgg tactgattgg tgaacccggc gtcggtaaaa ctgccatcgt
    25141 tgaaggtctg gcgcagcgta ttatcaacgg cgaagtgccg gaagggttga aaggccgccg
    25201 ggtactggcg ctggatatgg gcgcgctggt ggctggggcg aaatatcgcg gtgagtttga
    25261 agaacgttta aaaggcgtgc ttaacgatct tgccaaacag gaaggcaacg tcatcctgtt
    25321 tatcgacgaa ttacatacca tggtcggcgc gggtaaagcc gatggcgcaa tggacgccgg
    25381 aaacatgctg aaaccggcgc tggcgcgtgg tgaattgcac tgcgtaggtg ccacgacgct
    25441 tgacgaatat cgccagtaca ttgaaaaaga tgctgcgctg gaacgtcgtt tccagaaagt
    25501 gtttgttgcc gagccttctg ttgaagatac cattgcgatt ctacgtggcc tgaaagaacg
    25561 ttacgaattg caccaccatg tgcaaattac tgacccggca attgttgcag cggcgacgtt
    25621 gtctcatcgc tacattgctg accgtcagct gccggataaa gccatcgacc tgatcgatga
    25681 agcagcatcc agcattcgta tgcagattga ctcaaaacca gaagaactcg accgactcga
    25741 tcgccgtatc atccagctca aactggaaca acaggcgtta atgaaagagt ctgatgaagc
    25801 cagtaaaaaa cgcctggata tgctcaacga agaactgagc gacaaagaac gtcagtactc
    25861 cgagttagaa gaagagtgga aagcagagaa ggcatcgctt tctggtacgc agaccattaa
    25921 agcggaactg gaacaggcga aaatcgctat tgaacaggct cgccgtgtgg gggacctggc
    25981 gcggatgtct gaactgcaat acggcaaaat cccggaactg gaaaagcaac tggaagccgc
    26041 aacgcagctc gaaggcaaaa ctatgcgtct gttgcgtaat aaagtgaccg acgccgaaat
    26101 tgctgaagtg ctggcgcgtt ggacggggat tccggtttct cgcatgatgg aaagcgagcg
    26161 cgaaaaactg ctgcgtatgg agcaagaact gcaccatcgc gtaattggtc agaacgaagc
    26221 ggttgatgcg gtatctaacg ctattcgtcg tagccgtgcg gggctggcgg atccaaatcg
    26281 cccgattggt tcattcctgt tcctcggccc aactggtgtg gggaaaacag agctttgtaa
    26341 ggcgctggcg aactttatgt ttgatagcga cgaggccatg gtccgtatcg atatgtccga
    26401 gtttatggag aaacactcgg tgtctcgtct ggttggtgcg cctccgggat atgttggtta
    26461 tgaagaaggt ggctacctga ccgaagctgt gcgtcgtcgt ccgtattccg ttatcctgct
    26521 ggatgaagtg gaaaaagcgc atccggatgt cttcaacatt ctgttgcagg tactggatga
    26581 tgggcgtctg actgacgggc aggggagaac ggtcgacttc cgtaatacgg ttgtcattat
    26641 gacctctaac ctcggttccg atttgattca ggaacgcttc ggtgaactgg attatgcgca
    26701 catgaaagag ctggtgctcg gtgtggttag ccataacttc cgtccggaat tcattaaccg
    26761 tatcgatgaa gtggtggtct tccatccgct gggtgaacag cacattgcgt cgattgcgca
    26821 gattcagttg aaacgtctgt ataaacgtct ggaagaacgt ggttatgaaa tccacatttc
    26881 tgacgaggcg ctgaaactgc tgagcgagaa cggttacgat ccggtctatg gtgcacgtcc
    26941 tctgaaacgt gcaattcagc agcagatcga aaacccgctg gcacagcaaa tactgtctgg
    27001 tgaattggtt ccgggtaaag tgattcgcct ggaagttaat gaagaccgga ttgtcgccgt
    27061 ccagtaaatg ataaaacgag cccttcgggg ctcgtttttg tctataagtt agacggaaaa
    27121 gactatattt aagatgtttt gcctgaaaag tgagcgaacg ataaagtttt tatatttttc
//
LOCUS       DAZFFG010000064        27109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFFG010000064 DAZFFG010000000
VERSION     DAZFFG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.064"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            144..1208
                     /gene="ulaG"
                     /locus_tag="ACHJ7J_002938"
     CDS             144..1208
                     /gene="ulaG"
                     /locus_tag="ACHJ7J_002938"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO5001140.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1316..2071
                     /gene="ulaR"
                     /locus_tag="ACHJ7J_002939"
     CDS             1316..2071
                     /gene="ulaR"
                     /locus_tag="ACHJ7J_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO5001141.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2068..2817)
                     /gene="yjfP"
                     /locus_tag="ACHJ7J_002940"
     CDS             complement(2068..2817)
                     /gene="yjfP"
                     /locus_tag="ACHJ7J_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5001142.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDIPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2999..3328
                     /gene="bsmA"
                     /locus_tag="ACHJ7J_002941"
     CDS             2999..3328
                     /gene="bsmA"
                     /locus_tag="ACHJ7J_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO5001143.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3477..3752
                     /gene="yjfN"
                     /locus_tag="ACHJ7J_002942"
     CDS             3477..3752
                     /gene="yjfN"
                     /locus_tag="ACHJ7J_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO5001144.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3869..5494)
                     /gene="aidB"
                     /locus_tag="ACHJ7J_002943"
     CDS             complement(3869..5494)
                     /gene="aidB"
                     /locus_tag="ACHJ7J_002943"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO5001145.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5578..6741)
                     /gene="yjfC"
                     /locus_tag="ACHJ7J_002944"
     CDS             complement(5578..6741)
                     /gene="yjfC"
                     /locus_tag="ACHJ7J_002944"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5001146.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVERAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6744..7382)
                     /gene="yjfM"
                     /locus_tag="ACHJ7J_002945"
     CDS             complement(6744..7382)
                     /gene="yjfM"
                     /locus_tag="ACHJ7J_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO5001147.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7392..7790)
                     /gene="yjfL"
                     /locus_tag="ACHJ7J_002946"
     CDS             complement(7392..7790)
                     /gene="yjfL"
                     /locus_tag="ACHJ7J_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO5001148.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7808..8467)
                     /gene="yjfK"
                     /locus_tag="ACHJ7J_002947"
     CDS             complement(7808..8467)
                     /gene="yjfK"
                     /locus_tag="ACHJ7J_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO5001149.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8518..9216)
                     /gene="yjfJ"
                     /locus_tag="ACHJ7J_002948"
     CDS             complement(8518..9216)
                     /gene="yjfJ"
                     /locus_tag="ACHJ7J_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO5001150.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9235..9636)
                     /gene="yjfI"
                     /locus_tag="ACHJ7J_002949"
     CDS             complement(9235..9636)
                     /gene="yjfI"
                     /locus_tag="ACHJ7J_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO5001151.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDKFNTFLLRNQKMMPLSSVGISSVQQEAYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(9763..10494)
                     /gene="rlmB"
                     /locus_tag="ACHJ7J_002950"
     CDS             complement(9763..10494)
                     /gene="rlmB"
                     /locus_tag="ACHJ7J_002950"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO5001152.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(10675..13116)
                     /gene="rnr"
                     /locus_tag="ACHJ7J_002951"
     CDS             complement(10675..13116)
                     /gene="rnr"
                     /locus_tag="ACHJ7J_002951"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO5001153.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDTGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(13155..13580)
                     /gene="nsrR"
                     /locus_tag="ACHJ7J_002952"
     CDS             complement(13155..13580)
                     /gene="nsrR"
                     /locus_tag="ACHJ7J_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO5001154.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(13785..15083)
                     /gene="purA"
                     /locus_tag="ACHJ7J_002953"
     CDS             complement(13785..15083)
                     /gene="purA"
                     /locus_tag="ACHJ7J_002953"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO5001155.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(15187..15384)
                     /gene="yjeT"
                     /locus_tag="ACHJ7J_002954"
     CDS             complement(15187..15384)
                     /gene="yjeT"
                     /locus_tag="ACHJ7J_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO5001156.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(15466..16470)
                     /gene="hflC"
                     /locus_tag="ACHJ7J_002955"
     CDS             complement(15466..16470)
                     /gene="hflC"
                     /locus_tag="ACHJ7J_002955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO5001157.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(16473..17732)
                     /gene="hflK"
                     /locus_tag="ACHJ7J_002956"
     CDS             complement(16473..17732)
                     /gene="hflK"
                     /locus_tag="ACHJ7J_002956"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO5001158.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(17818..19098)
                     /gene="hflX"
                     /locus_tag="ACHJ7J_002957"
     CDS             complement(17818..19098)
                     /gene="hflX"
                     /locus_tag="ACHJ7J_002957"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO5001159.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(19175..19483)
                     /gene="hfq"
                     /locus_tag="ACHJ7J_002958"
     CDS             complement(19175..19483)
                     /gene="hfq"
                     /locus_tag="ACHJ7J_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO5001160.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(19569..20519)
                     /gene="miaA"
                     /locus_tag="ACHJ7J_002959"
     CDS             complement(19569..20519)
                     /gene="miaA"
                     /locus_tag="ACHJ7J_002959"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO5001161.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(20512..22359)
                     /gene="mutL"
                     /locus_tag="ACHJ7J_002960"
     CDS             complement(20512..22359)
                     /gene="mutL"
                     /locus_tag="ACHJ7J_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO5001162.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(22369..23706)
                     /gene="amiB"
                     /locus_tag="ACHJ7J_002961"
     CDS             complement(22369..23706)
                     /gene="amiB"
                     /locus_tag="ACHJ7J_002961"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO5001163.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(23725..24186)
                     /gene="tsaE"
                     /locus_tag="ACHJ7J_002962"
     CDS             complement(23725..24186)
                     /gene="tsaE"
                     /locus_tag="ACHJ7J_002962"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO5001164.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(24158..25705)
                     /gene="nnr"
                     /locus_tag="ACHJ7J_002963"
     CDS             complement(24158..25705)
                     /gene="nnr"
                     /locus_tag="ACHJ7J_002963"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO5001165.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDAMLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTEKNHD
                     ESSNSAP"
     gene            25704..26843
                     /gene="queG"
                     /locus_tag="ACHJ7J_002964"
     CDS             25704..26843
                     /gene="queG"
                     /locus_tag="ACHJ7J_002964"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO5001166.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKKPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(26826..26879)
                     /locus_tag="ACHJ7J_002965"
     CDS             complement(26826..26879)
                     /locus_tag="ACHJ7J_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001167.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         6491 a         7508 c         6669 g         6441 t
ORIGIN      
        1 atctttccat actccaaatt cacaacaaat cacccggcaa aaagtcaaaa aatggtcatt
       61 gccttcctga aaaaatagtg tcaggattaa tcaaaaccaa tcatatgttg attcgaattg
      121 aactatcatg aaggtaaatg gcgatgagta aagtgaaaag tatcacccgt gaatcctgga
      181 tcctgagcac tttcccggag tggggtagct ggttgaatga agaaattgaa caagagcagg
      241 tagctcctgg tacattcgcg atgtggtggc tcggttgtac cgggatctgg ctgaaatcgg
      301 aaggcggtac caacgtctgc gtcgattttt ggtgcggtac tggcaaacaa agtcacggta
      361 acccgctgat gaaacagggt caccagatgc agcgtatggc tggcgtgaaa aaactgcagc
      421 cgaacctgcg cactacgccg tttgttcttg atccatttgc tattcgccag atcgacgccg
      481 tcctggcgac tcacgatcac aacgatcata tcgacgtaaa cgtcgctgct gccgtgatgc
      541 agaactgcgc tgatgacgta ccgtttatcg gaccgaaaac ctgcgtggat ttatggatcg
      601 gctggggcgt gccgaaagaa cgttgcatcg tggttaaacc gggcgacgtg gtgaaagtca
      661 aagacattga aattcatgcg cttgacgcct tcgaccgtac tgcactgatt accctgcctg
      721 ccgatcaaaa agcggcaggc gtgctgccag acggcatgga cgatcgcgca gtaaactacc
      781 tgttcaaaac gccgggcggt tcgctgtatc acagcggcga ctcccactac tccaactact
      841 atgcaaaaca tggtaacgaa catcagatcg acgtggcttt aggatcttac ggtgaaaacc
      901 cgcgcggtat taccgataaa atgaccagcg ccgatatgct gcgtatgggt gaagcgctga
      961 atgcgaaagt agtgatcccg ttccaccacg atatctggtc aaacttccag gccgatccgc
     1021 aagagatccg cgtgctgtgg gagatgaaaa aagatcgtct gaagtatggc ttcaagccgt
     1081 ttatctggca ggtgggcggc aaatttacct ggccgctgga taaagacaac ttcgagtacc
     1141 actatccgcg cggtttcgat gattgcttca ctattgaacc ggatctgccg ttcaagtcat
     1201 tcctgtaatc tgtcgtttat accggatagc gtggagaagt tcgcgctatc cggcgtagta
     1261 tttcctggct atttcaaata tcatctaaaa aaatcaaatt ttatcggaat agctcatgac
     1321 tgaagcacaa agacatcaaa ttctcctgga aatgctcgca caattgggct ttgtgaccgt
     1381 tgagaaagtc gttgagcgtc tgggaatttc gcctgccact gcgcgacgcg atatcaataa
     1441 acttgacgaa agcggcaaac tgaaaaaagt gcgcaatggc gcagaagcta ttacccaaca
     1501 gcgcccgcgc tggacgccga tgaatctgca tcaggcgcag aatcacgatg aaaaagtacg
     1561 tatcgctaaa gcggcctcgc agctggttaa tccgggcgaa agcgtagtca tcaactgcgg
     1621 ctccaccgcg tttctgcttg ggcgggaaat gtgtggcaag ccagtgcaaa tcatcactaa
     1681 ttatctaccg ctggcaaatt acctgatcga tcaagaacat gacagcgtga tcattatggg
     1741 cggacagtac aacaaaagtc agtccatcac tttaagcccg cagggcagcg aaaacagtct
     1801 ctatgccggg cactggatgt ttaccagcgg aaaagggctg accgcagaag ggttgtataa
     1861 aaccgatatg ctgacagcaa tggcagagca gaagatgctg agcgtggtag ggaaactggt
     1921 ggtactggtt gatagcagta agattggcga acgcgcggga atgcttttta gccgtgccga
     1981 tcaaatcgat atgcttatca ccggcaaaaa tgctaacccg gaaatcctgc aacaactgga
     2041 agcgcaaggg gtcagcattc tgcgtgttta aagatgctgg cggaaaaatg tcacggcagc
     2101 atctaacgcc tcaggcgtaa tgcggtgacg cacgcctggc tgccatgaac aggttagcag
     2161 tttatcccgt cccgtctcgc ttaaggcctg ctgcaaacgt agtgattcgt cggcaggcac
     2221 aacgtcatcg tcgaggccat gccacagcag tagaggtctg tcactaagtt gttccaggtg
     2281 gtttgtcgct tcccactctg ccagtggcgc gacaatgtta ttgaattcat tctgctgtgc
     2341 tgccgtttca ggtatcagcg gtggaaacag tgaacgggcg agtgatgtaa aatagcccga
     2401 tcccatcatg ctggcggtac atctcaccgt ggggtggcga gcggtaatcc ccagtgccgt
     2461 catcgcgccc atcgacgcac caccgactgc cagacggtca tcaagcagcc agttttcttc
     2521 ggctattgcc gcacgtaaag tagtgaattc ctgcatactt tgtagcaaga tttgccagaa
     2581 ttgatttaac cgccgcgctg cgtcaccact aaaacggcta ccgtgatcgg gcgcatccgg
     2641 catgatcacc cgcaaaccag cctgcgccag cgcaacggca aaatagctat acaccagact
     2701 ggacgaagta aagccgtgat aaaaaatcgc gcacggtaac gggatatctt tttgtccgac
     2761 aggataagca tgaagaacgg gaatatctgc cagctcgcgt gattctattt caatcatctg
     2821 tctctccttt ttgtctggta tgacatgaag aaatccttag cacaagtttc tgctggcggt
     2881 attagttcat aatgttgaga tgtgggttac gctttcgtca cgttttcatt cgaaaatcgc
     2941 gtaccaggta acaattcggg aacattcccc caaagttaaa ttaataaggc actacactat
     3001 ggttagcagg aaacgtaata gcgttattta ccggtttgcc agtttattat tggtgttgat
     3061 gttaagtgcc tgtagcgcac tgcaaggtac gccacagcca gcaccgccag taacggacca
     3121 tccgcaagag attcgccgcg accagacgca agggttacag cgaataggta gcgtaagcac
     3181 gatggttcgg ggttctccgg atgacgcatt agcagaaatc agagcgaaag ctgtcgctgc
     3241 aaaagctgat tattacgttg tcgtcatggt ggacgaaacc atcgtgacag gacagtggta
     3301 ttcacaagcc attttgtacc gtaaataaat cagacaactt acatagtcag ctttacattg
     3361 ctttgcgtcc ctatgcgttt acctgtgcac aataaattac cggctggaag cctgggcgtt
     3421 tattaccgcg atggaatgcc ctgcaacatg tggggaaatg aaaaatggag ctgacgatga
     3481 aacaattact tgcctcaccc tcgctgcaat tagtgactta tcctgcgagc gccacggcac
     3541 agtctgcaga attcgccagt gctgactgcg tgacgggcct gaatgaaata ggtcagatct
     3601 cagttagcaa tatttcaggg gatccgcagg acgtggaacg tatcgtggcg ttaaaggccg
     3661 atgaacaagg tgcgtcatgg taccgcatta ttaccatgta cgaagaccag cagcctgata
     3721 actggcgtgt acaggcgata ctgtatgcgt gagtgtcatc tacagcaaat gaacaatgcc
     3781 ggatgcggcg cgagcgcctt gtccggccta caattttcac atatttcatt gagttaatcg
     3841 aaaccagcgt cgcatcagtc gtatacgctt acacacacac tccccccgtc gcccgcagca
     3901 ataaatcatt ctggatctgc tctgacaacc gtacgccgcc gcgcgtatct aacatcacct
     3961 gacaccacgc ctgcgccatt ggcggagagg catatttcaa catttgcgca ccgcagccca
     4021 gcaggaatag ctgatgagta atctctcgcc ccagttcttc agctggctta cgcagctgct
     4081 gctgtaaacg acgaaccgcg cgatcaaaat agcgatcctg ccctttcact tccacaaatg
     4141 cttccgacaa taagtcgtat acgcccgctt gcttattgag aacgcgcaac acatccaggc
     4201 acataatatt gccggaacct tcccaaatac tgtttaccgg catctcccgg taaagccgcg
     4261 gcagctcgct ctcctcgcaa taaccaatgc cgcccagcac ctccatcgct tcggccacaa
     4321 acggcatacc tcgtttgcag atcacaaatt ttgccgcagg cgtaaataaa cgcgcccaca
     4381 gggcttcttt ggcatcggca cgccggtccc acgctcgcgc aagacgaaac agcaacgccg
     4441 tttgcccttc aagctgaagt gccatgcgac ttaagacatg acgcataagg ggctgttgga
     4501 tcaatggatt accaaaaaca tggcgttgat gtgcatgata aatcgccagc gaaaatgcac
     4561 ggcgcatcat ggcatggcta cccagggcgc aatcaaaacg cgtcatcccc cccattttca
     4621 ggatcagacg aattccttcc ccttcctgcc ccaataacca gccaatggca tcctgaaact
     4681 ccacttcgca actggcgtta gagcgattac ccagcttatc tttcagccgc tcgaggcgaa
     4741 tcgcgttgcg ttgcccgtca ggcaaaaagc gcggcacaaa aaagcaggac agaccgcccg
     4801 cggtctgcgc cagcaccaga tgcgcatcgc tttgcggaac cgagaaaaac catttatgcc
     4861 ccaccagccg ataagagcca tcttccagac gctctgcacg ggtggtgttg ctcatgacat
     4921 cggaaccgcc ctgcttttcc gtcattccca tgccaatcaa caaacctcgt ttttgcccac
     4981 ctggcaataa atgagaatcg tagcgatcgc tcagcagcgg cgtggtccag tcctgaaacg
     5041 gcgcgggtaa catctgtaac aacaatggcg tggcggcaaa ggtcatcgtt attggacata
     5101 acgacccagc ctcaacctgc gcatgtaaca taaaacgcgc cgcgcgcgcc acaaatgcgc
     5161 cggagcgagc gtcttcttcc caggcaagat tgtgcacccg attggtacat agtgcctgca
     5221 tcagcaggtg ccaggcgggg tgaaaacgca catcgtccag acggcgtcct tgcgcatcgt
     5281 agcgcaataa ttcaggcgga ttcacattcg ccagccgccc cagttcaagg gattcagccg
     5341 ttcctaactg ctgaccaata ctggctagaa aatcgctatc ccagccagca ccttcacgcg
     5401 ttaccgcttc gcaaagcgcg ccatcagaca ggtataagtt gctgttattt aatggtatag
     5461 gttgattaaa aacggtgtga gtttgccagt gcactgtgtc tccctccatc aatggcagtc
     5521 accattagta tggtcactgc catggattca tgacagaaag agattgctaa aacattctta
     5581 tccagcaatg taatgcggaa cgaaacgtga ggtgtctttg gtgatcagtg tgttatcttc
     5641 acggatcccc attccgcacg cttcatcatc gacaatccag ctaccgatga gtgtgtagct
     5701 atcgccaaac cgcggcagag gttgaaacgc ctggtagatc atcggttcat cggcgtaatc
     5761 accatcagcg tggtcaacga cgttattctg gccgtcgaaa atggtgacat ttccgccttc
     5821 gcgcgagtag atcggtttac gcacatagct ttcgccagcg gcaatctgcg gtttttcacc
     5881 ctcaaaccag gatgcaagaa ggttaggatg accagggaag aagcgccaaa gcagcggcat
     5941 tagcccttta ttactcaaga tacttttcca taacggctcc acccattgct cgcgacgctt
     6001 gcgcagcagc ggaccattat cgtcacgcat catccactcc agcggataca gcttaaatgc
     6061 acgctggatg acattatcat caagatcggt cagtacgccg ccgacgccca aaccgagatc
     6121 ttcaatgtag ataaaccgcg actcctgccc tgcctgctgg gcgcagtcct gcaagtacag
     6181 cacggtactc ctgtcttcat cggtgtcctg acagcagcaa aaataaaacg gttcccgact
     6241 gtaaagctcg ctaaagcgtg aaatcaggcg ttcctgaata gcattgtact gatcggcatc
     6301 acgcggaata atgccgctgc gccgggcatc ctctagccac agccactgga aataagccga
     6361 ctcatacaac gaggtcggcg tatcggcgtt gtactccagc agcttcaccg gcgcattgcc
     6421 acaccaggca aaatccatcc ggccatacag cgaaggatcg cgtgcgcgcc agctttcagc
     6481 gatcacatcc cagtacaacg gagggatcgc cagttgcgtc aggatctctt cgtctttaac
     6541 cgcgcgctcc accacctcaa ggcacatctg atgcagttcc gcagtcggtt tttcgatctg
     6601 ctcttcaatc tggcgcagag taaaacggta agcccgactc tcatcccaat agatttcatt
     6661 gtcgatgata tgaaagtcga aaccgttgtc agcggcgatc tggtccagat cccgtcgcac
     6721 aggaacgttg tgtctcagca tgattagcct ccccaatgcc cacgggcgct ggaagaacga
     6781 ccatagccgc cgcgagaaac ggttgacgct tttttggtgg taaagccgcc tgaagagtaa
     6841 gactcttttt tgccagaacc ggagcgccag gagtaatcac cagaagtgtt gcgccagacg
     6901 gggcgcgaag caaaagagga accgccgctg ttatagacga acggctcatc gcgatcttta
     6961 cgaataaccc ggcttaacaa aaatccagaa acgaccggga tccaactctg ttcaacattg
     7021 tcgtagtagc aattttcgta cttagactga cagttctgct gagtcatatt cttcggaaca
     7081 tcggcataaa atgccgcttt ggcgttgttc cagccacgcg cgcaaatgtc ggcattatta
     7141 ccgtcatcaa tacaatcctg cactgtcgaa taaaacgttc cgtcgccgtc gttatcgaca
     7201 tcgccactat cgccacaacc tttcaataca aaaaaagcgg caccgcccat tagcgccaac
     7261 gttaagtatt tttgtgcgta gcgattgcga cgcggttcaa aaggattatt cggtctacca
     7321 atgcgactga tcgctccgtg accaatttta ctgttatttc ttgatttgcg ttttctggcc
     7381 atgatcgtcc attaccatgt catacaagct gcgttaaaaa taccgccagc cagcgcggcg
     7441 gttcccataa acattcctgc tgcggtattg tgattaataa ttttttcgct taatgcgggc
     7501 atataaagtc tgacaccagc aaaaacgagt aactgaatca ccagtgcaat cccgccccag
     7561 gcgaaatagt ctggaatact caccgcattg attgccgcac tggataaggg gatcacgtaa
     7621 cccaacaatg taccgctgaa cgccagtgac gctgcggtat tattgttttt gattaattgc
     7681 cattcattgt gcggtgtaat tttggagtag ataaacagaa aaataatcac catcgccacg
     7741 ccaataaaaa aataggcact aaaggcaaga agtgaatcca gtatatgcat tgtaaacgtc
     7801 cttgtaatta accaataata tgaagtgatg tcagcgggat atcgacaccc aacgcgcgcg
     7861 aaaataacca ctctggctcg ccgagatcgt taaaagattc ctcaccattt agcagcaaat
     7921 attcgtaagt atcttcggtc acttgccgct ggtagcccat ggtaaaatta tggacttccc
     7981 atttagcatg attttgattt tctacttttt ccagcatgta gacgggttcg atatttcccg
     8041 gttcttcgct gttaaaaaat cgctgccagc gtttttcctg ccagtttaag gtcattgccc
     8101 ccatcgtttt ggcgttgatc gcctcgcgcc agtgactctc tttactgata ccgtagctct
     8161 cttcatagac aaagagcttg atatcatcaa tgtcatcaat atcttcgccg ccggtggtat
     8221 tgatttgcag aaactcatcg cctgaagtgt agtagcggaa aatctggcta ccgccgccca
     8281 gatcgatgtg actgacagcg gctaccgtaa attcctcacc cggcagcgca atcagcaatt
     8341 catcttccag caaacgaaac gctaacgtgt cgagcgtaaa gccactattg agatgaagtc
     8401 ccagcggacc gcgagcgata gcaggcttat tatccttgcc aaacagacgc tggaaaaaac
     8461 cagacatatt ttatccttaa aatagttgcc agccttgcag tgaggctggc aaaaaaatta
     8521 ctcgccctgt tggcgttgca gtcttgctaa tacatcctgg gcgctacttt tattgctacc
     8581 gccaattccg gcttccgcca gcttttcgtc aaggtcgcga ccgtctgcga ctttctccaa
     8641 ctgtgcggca gcatccaggc gagcctgacg ttctgcctga cgcgtttgca ggcgttttaa
     8701 ggattctgcc gctgtcgaaa cgctggaaga tgcgccaacg gtagaggttg ttacagcctg
     8761 ttgtgcacgc tgcatggctt cagtggcttt aacgacttcc atttgctgct caaactgagc
     8821 gatacgctgc gctgtcgctg taactgcttt ttccacgcca tcacgggaga cttccagatt
     8881 cgacaacact tgctcttcag cggtaatgag attctcaagg cgcgcgattt cttcagcaac
     8941 ttcgttaatc agcgacggat taacgttctt gcttaacgct tccagcgcac gggcttccag
     9001 actggctttg cgctcacgta aatctttcag cttatcgtga cttaatttca cccgcgccag
     9061 cagatcaacg cgagattttc cggctttatc gagttcagct ttagcgtcac gaatatgctg
     9121 ttccagcatg cgcacaccct gggtttcttc aatggattct tccgcctggg agataaacga
     9181 tttccccagc gtaaataaac tttttaaaat tcccataaat acccgtcctt gtaattagtg
     9241 tgaatattct tctgtaattt cagccagatc caatgcgtta tctaccagcg aggttatctc
     9301 aagcaggata tcttcaagag aagatttaag cgataacgcc ccgaaaacaa tgtaatacgc
     9361 ttcctgttgc acgctggaga ttcctaccga tgacaacggc atcatcttct gatttcttaa
     9421 taagaaggta ttaaatttat ctggattgct gatactactt accggacaaa taaaggtttc
     9481 gataatcatt tggcgggaag taaaaagaat attgatttgc aaatcgccat aatcattcat
     9541 tttaataatt aatgcgttct cgctatttgt tacatcaata ttttgttcag gtacagtttg
     9601 cagcgccgtc gccagcgcca acggattcca tgtcatatat tattccatat agattaagtt
     9661 taaatattaa taaaatgaat atttgcaata cgtaattatc ttaccagcta tagacaaaaa
     9721 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
     9781 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agagaagaaa cacttcctgc
     9841 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
     9901 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
     9961 cgcctcacct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
    10021 cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc gcgccgcagg ctactttttt
    10081 cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcgt gaacgccagc
    10141 agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga tcggttacac cgtcgaggat
    10201 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
    10261 acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
    10321 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
    10381 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
    10441 ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt
    10501 actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggtctacatg atccctgcaa
    10561 tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga
    10621 acaaagcgca ttttgtcagc aatctaaccc tcttctgttt gaagagggca atgatcactc
    10681 tgccactttt ttcttcgccg cacgcttcgc tttggtcgct gcagctattt tctgcgtttt
    10741 cgccgatggc tttttcgctt ttctcgcgtc tttcttcgct gctttcggct tcgttttttt
    10801 ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt ttaccgacct gacgacgctt
    10861 gccgcctttt ttacctgtat cgcctttttt cgctttctcg cgcgccgttt taccgacgtt
    10921 gcgcggtgcg cgttcgctgg agatcaggct aaagtcgatt ttgcgctcgt ccatattaac
    10981 cgcttcgacg cgaacttcca cgcgatcgcc cagacgataa gtctggccgc tggattcacc
    11041 catcaggcgt tgccctacct ggtcaaagcg atagtagtca ttgtccagcg aagagacatg
    11101 gaccagacca tcaatgaata agtcgtccag acggacaaag aatccaaagc cagtgacgct
    11161 ggaaattacg cctttaaaga cgttacctac ctggtcgagc ataaagtcac acttcagcca
    11221 gtcagccaca tcgcgcgttg cttcgtcggc acgacgttct gccatcgaac agtgctgacc
    11281 cagttgcagc atctcttcca tcgaataatg gtagccgccg gtttcagtgg tgttaccctg
    11341 atgcccctgc tctttcgcca gcagatactt aatggcgcgg tgcagcgtca ggtcaggata
    11401 acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccagaccga agtgaccacg
    11461 gttttccgga tcgtaaatcg cctgtttcat cgagcgcagc agcatggttt gcagcatttc
    11521 tgcatcagga cgatcggcaa ccgactccag caactccgcg tagtcgcgcg gttccggctt
    11581 gttaccgccc ggcagttcca gccccagctc cgccagcact gaacggaaag aggtaatcgc
    11641 ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttggctt tctcaacgaa
    11701 acgcgccgcc gagatattcg ccagaatcat gcactcttca attaacttgt gtgcgtcgtt
    11761 acgctgagtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga acttcgcttc
    11821 ttcgctctca aatgagatcc cgccgcgttc ttcacgggct ttatccagca ctttatagag
    11881 gttatgcaac tcttcgagat gcttaaccag cggggcgtac tgctcgcgca gatcctgatc
    11941 gccctgcaga atatgccaga ctttggtgta ggtcagacgc gcgtgagagc tcatcaccgc
    12001 ttcgtagaat ttgtagccag tcaggcggcc tttcgacgaa accgtcatct cgcacaccat
    12061 acacaggcgg tctacctgcg ggttgagcga acacaggccg ttagagagca cttccggcag
    12121 catcgggata acctgcgaag ggaagtacac cgacgtgcca cggttacgcg cttctctgtc
    12181 cagcggcgtt ggcggacgca catagtagct gacgtcggca atcgcgaccc ataaacgcca
    12241 gccgccgccg cgttttttct cgcagtaaac tgcatcgtca aagtcacggg cgtcttcgcc
    12301 atcaatggtg accagcggta aatcgcgcag atcgacacgg cccgcttttg cttcttccgg
    12361 cacttcttct ttcagcccgg caacctgttg ctcaacagcc tgcggccaga tgtacggaat
    12421 ttcatgggta cgcagagcga tatcaaccgc catgccggtg cccatattat cgccaaggac
    12481 ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga cgctgagtca gttcgactac
    12541 gaccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga tatcgaagct
    12601 cagacggctg tcgtcaggaa ccacaaagcc gacgcccgct tcggtaaagt agcgaccaac
    12661 aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac gacctttacg
    12721 gtcagcaccc agcggctgag ccagcacctg atcgccatga atgcaggttt tcatctgctc
    12781 gctggagaga tacaaatcat ctttacgccc ttcaacccgc agaaagccgt agccatcacg
    12841 gtggccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat agcactgacg
    12901 acgagtgaag accagttgac catcgcgctc catcgcgcgc aggcggcgac gcaggccttc
    12961 aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc tggccggttt
    13021 ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat tcgcgtattt
    13081 ttcagcttcg cgttcctgga aaggatcttg tgacatctcg gttcctccgt tgtcatctct
    13141 gatgaagatt ttcgtcactc caccagcaat aatttataaa gcggttgatt ctcttcaacc
    13201 aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc cttagaaagt
    13261 gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca attcaccagc
    13321 gataagggct ccagctcgcg caccacatca ccaatacgta tcgcactcgc cggtttaccc
    13381 aggcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg actaagttga
    13441 ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac ttcagaaata
    13501 ctggtcatcc gcccttctgg caatgacgcc atgtagatca gcgcacgtaa tccgtaatca
    13561 gtgaaactcg ttaactgcac atcaacctca aaagggaaat cgggaaaaaa tacatttata
    13621 ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg gaaggaaaaa
    13681 agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag caatgtatca
    13741 cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc gtcgaacggg
    13801 tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc gatcggcaca
    13861 ccagtcagct cttcaatgcg cttgatgtag ttcagcgccg cctgcggcag gccgctacga
    13921 tctttcacgc cgaaggtgga ttcagaccag cccggcatgg tttcgtaaat cggctctaca
    13981 cctttccagt cgtcagctgc cagcggagtg gtagtcactt cacgaccatc cggcatacgg
    14041 taagccacac agagtttcac ctctttcagg ccatccagaa cgtccagttt agtcaggcag
    14101 aagccagaca gggagttcag ctgtaccgca cgacgaacgg caacggtgtc cagccagccg
    14161 gtacgacgac gacgacccgt agttgcgccg aattcgttac cctgcttgca gaggaactcg
    14221 ccagtttcat caaacagttc agtcgggaac ggacctgcac ccacacgagt ggagtaagct
    14281 ttgaggatac ccagaacgta atcaacataa cgcgggccca ggccggaacc ggtcgccacg
    14341 ccaccagcag tggtgttgga agaagttacg tacggataag taccgtggtc gatatccagc
    14401 agcgtaccct gcgcaccttc gaacatgacg aaatcgccac gctgacgcgc ctggtcgagc
    14461 aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg caacagccat cgtatcatcc
    14521 agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg gaagttgtga
    14581 tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag gtcgccaaca
    14641 cgcagaccgc gacgtgccac tttatcttca taagcaggcc cgataccacg accggtggtg
    14701 ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagcgcaac gtgataatca
    14761 aggatcagcg gacatgcttc agacagcagc agacgctcac gaacggggat gccacggtct
    14821 tccagttctt tcatctcttt catcagcgcg gccggagaca gcacaacacc gttaccgatg
    14881 atgctggtta cattctcgcg gagaatacct gatggaataa gatggagaac ggttttttca
    14941 ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac aacatattta
    15001 gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca ttgggtgccc
    15061 agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa tggattctac
    15121 catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga tcagtctgtt
    15181 ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg caaccacaag
    15241 tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag agatcatctt
    15301 cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc agtaccaaaa ccagggcaag
    15361 cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac caccgacatc
    15421 cctgaggatg cggtggcttt attgacctgt accgcagtcg ttatattaac gcgttgcgga
    15481 agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg ctcatgacca tcacgtcctg
    15541 attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt agaagtccgg
    15601 atctttactg aatgcatcag caaacagttt ggctgcttcg gcatcgcctt caccacgcat
    15661 gatacggccc tgacgctcag cttctgccag cgttctggtc acttcatagt cggcagtcgc
    15721 gcgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta ccgcttcacg
    15781 ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt tgatctgctt
    15841 gatacgcaca tcgacaactt caatacccag cgccgccata ctgttcgggt tgatgaccgg
    15901 aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg cgttatctgc
    15961 cgccggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg cgtcacgtac
    16021 ttcgagggtc agacgaccac gggaatcagt gacgatatct ttcacgtcca ggcgaccaat
    16081 ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt gcgaaatgtc
    16141 gccaccaccc gttgccaggt agtaacggct gaaatcgctg atgcgccatt tgatgtaaga
    16201 gtcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt tgtccatggt
    16261 ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga aatgcagacc
    16321 cggctcataa accagaggtt tgttgtcatc gtcacgcagt accttaccaa aacgcagcgt
    16381 aataccgcgc tcaccttctt tgacgacaaa gacagacatg taaagcacta ccagcacgat
    16441 gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg gtagtcgtta
    16501 cgctgcgcgt tggcgcggcg ttggtccata atatcgccct gactggtgga cgacgtgttg
    16561 cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt gctggcaccg
    16621 ttatcgctct tcgccgcagg ggcgttacca cctttcagca tctggtctaa cggcagaacc
    16681 atcaggttgc cacctttgtc gttaaccaac actttgcggg tgttgcccaa cactttttcc
    16741 atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata ttccggcaga
    16801 agtttagcaa agcgcgccac ttcaccctga gcttccagga tggtctgggc cttgtacgca
    16861 cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac ttcgttggta
    16921 tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat cgcatcgtca
    16981 aacgccgctt ttacttcttc cggcggacga gcagcctgga agttgacgtc cagcagcgtg
    17041 atacccatgt catacggacg aatcgtctct tccagttcgc gctgagtatc gctacgaatc
    17101 acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac tccacgcagg
    17161 gcgctgtcgg tagcctgacg caggctgtca tccgggctgg tcacgctata cagatatttt
    17221 tccggattgg tgacacggta ctgcacgttc atttcaacgc gcactacgtt ctcgtccgac
    17281 gtcagcatca caccggaagc ggccagttca cgcacagctt ccacgttcac cggtttgact
    17341 tcgtcgataa acgtcggttt ccagttcaga cccggctcaa ccaaatggct gaatttacca
    17401 aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact ggccgcccag
    17461 ataatgacaa tcgctgccgc tgcgatggta acgacacgac cgccaagctg cgggcgcggg
    17521 ccttgcgatg aactgccacc gccagatccg gtgcctttac cgccgcccag accaccgagc
    17581 tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc gcgaccgcct
    17641 ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg gtcgcggtct
    17701 tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg ttatgcggtg
    17761 atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc tacgccgtta
    17821 gatcaggtaa tcgatcaacg ccggttcttg tttacagagg cgacgccagt caacgatcgg
    17881 catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt ctattgcctg
    17941 aagctgataa aaacgacttc tcagacgccc ttcctgcggc ggcaaacgca atgtatgctg
    18001 cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa agctgtggta tccccgctcc
    18061 ggtctgtgcg gaaagccaga cacggatcgg tttgttctct tcgtcccgat caatacgcgg
    18121 ttcgaaatct tccagcatat cgatcttgtt catcaccagc agggttggga tctcgtgagc
    18181 gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac gcacatccgc
    18241 cgcatcaatg acatgcagca gtaatgtggc ttgccgcgtc tcttgtaacg tggctttaaa
    18301 tgctgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg ccaacacggt
    18361 ttcaccgaca tctgcaacgt caatacgccg caacgtcggg tcgagggtgg caaacaactg
    18421 gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agggtagatt taccggcgtt
    18481 ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt gccgcccctg
    18541 ctcacgctgc ttttcaactc tttccaggcg cgactgtatc tgcacgatgc gattacgcaa
    18601 caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccta tcccgccttt
    18661 ctgtctttca aggtgggtcc agccacgcac caggcgcgta gccagatggc gcagctgcgc
    18721 cagctcaacc tgcaacttac cctcatgggt acgcgcacgt tgggcgaaaa tatctaaaat
    18781 aaggccggtg cggtcgataa cacgacactc gcacaaacgc tccaggttac gctcttgcgc
    18841 cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc gtcgctttga cagcttccgc
    18901 aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt tacggctacc
    18961 ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa actcctggag
    19021 gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct gctcaccagc
    19081 atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa cgcaggatcg
    19141 ctggctcccc gtgtaaaaaa aacagcccga aaccttattc ggtttcttcg ctgtcctgtt
    19201 gcgcggaagt attctgcgcg ctgctaccat gatggtagtt actgctggta ccgccaccgg
    19261 cgttgttact gtgatgagaa accgggcgag acgggacaac agtagaaatc gcgtgcttgt
    19321 aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt
    19381 gcccttgcag cttaatacca ttcaccaaat aaatagaaac tggaacacgt tcccgacgca
    19441 gtgcgttcag gaacggatct tgtaaagatt gccccttagc cattctctct tttccttata
    19501 tgcttatttg tactttgaac ctttcgattc tgaaaaaatt gcgcacgata cgtctcaatt
    19561 gtacacattc agcctgcgat agcaccaaca acctgtaata cttcgtcacg cgcctgttct
    19621 ggtttttcac tgtcaagcca gtgaacccct tcccaaccac gcagccaggt tatctgccgc
    19681 ttcgccaact gtctcgtggc gcaaacacct cgataaacca tttcatcgta tgagatttcg
    19741 ccttcaaggt aagaccacat ctggcgataa cccacgcaac gaatggaagg caagtccgta
    19801 tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt caaaacctga agccaacatc
    19861 tgatgaaaac gctgctcaat tcgttgatgg agcagttcac ggctcgccgg ggcgatggcg
    19921 aactgatgca cctgatacgg tagagcgtct cctgacgttt gcgtcagttc cgttaaagtt
    19981 ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc tttgtggatc atttggatga
    20041 atccttgctg ccgcaaccgg atcgatctcc tgaagttgac gatgcaatga ctcccaacct
    20101 tgctctgccg cctgttgctc aattctggcc cgtacttccg ggtctgccga cggtagcggc
    20161 gacaaccctt ccagcaatgc cttgaaatac aacatcgtac cgcccactaa cagtgggatc
    20221 cgccccgccg cggtgatatc ggccatttcc gccagcgcat cgcggcgaaa atcagcagcc
    20281 gagtaagcct gcgacggatc gcgaatatcc agcaatcggt gcggcgcggc gagtaactct
    20341 tcagcgttcg gcttcgccgt cccgatatcc atccctttgt aaataagggc agaatcaacg
    20401 cttatcaact ctactggtaa aattttacgc agttcaatgg ctaacgccgt tttaccggag
    20461 gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg ccttactgat atcactcatc
    20521 tttcagggct tttatcgccg gatgtaaatc aacagattgt aacagaccac ccggcggcgt
    20581 tttcacaagt tgcggacata accgttccac gtccgccagc agggttatgg cctgtgccat
    20641 tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc cactgcgcaa tattgccagg
    20701 ttcgaatacg gactgcttcg ccaggtagcc tatcagttca ggaatcaaga tttgtaaatt
    20761 ttgttggcgt aagggtaaag gcacggccct gatggtcaca tgctgtgcat ctgactggaa
    20821 atcaataccc aattccgcca gggcagactg cgctttttct aatgccgatt tttcttcgcc
    20881 agaaactttt agccgcaacg gaataagcag cggctgggcg caaacgggcg cttcacccgg
    20941 cgtcagttgc gcctgacgca gccaacgttc tgccactggc aaggctaaaa gtgaaatgtt
    21001 gccgtcgcgc tccagcaacg cacagtcgga atggacgata gtcagtaccc gaccaaaact
    21061 ctgactgttc gccgcaagtg caggttcctg cggttctggc gcttttggtt tttgcatcgg
    21121 cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc tgtttctggt aacctggctg
    21181 cgcattcggc cagggggcag ccggacgact gcctgaggcg ggcgcaggag tgtaacgcgg
    21241 agctaccggc tcacgagctg ccggttcagc aaaatgattg cgccccgccg ccacgcggtt
    21301 ttccggaata gcacgcggtg caggttgggg ttcatcgtcc agcggtagcg gcgtttccag
    21361 ttgctgttgt agcacgctca gcacgccctg atagataaag tcatgcacca gacgcgactg
    21421 atgaaaacgc acttcgtgtt tggcggggtg tacgttgacg tccacctgat gtgggtcgat
    21481 ctccagatac agcacaaatg ccggttgttg atcggccccc agtttgtctt cacaggcctg
    21541 gcggatcgca tgattgatca ggcgatcgcg catcatgcga ccattcacgt agcaatactg
    21601 aatttctgcc agggcgggcg tggtgtgatt cggatcggcc acccagccgc gcagggtgag
    21661 atcgccgtgt tgccattcaa tcgccagcgc ttgttcgaga aaagcggtgc cgcaaatcgc
    21721 gcctaagcgc cgttcttttt gcccgccttc cggcactgca cggtactgac gcacaatttt
    21781 accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc agcgcaatgc ggcggatgat
    21841 ttcatcgata tggttaaatt cagttttctc ggtgcgcagg aatttgcgcc gcgccggggt
    21901 gttgtagaac agatccagca cctccagcgt cgttcccacc ggatgcgccg ccggtttaac
    21961 cgtcacgtcc atatcgcgcc cttcggcata ggcctgccag gcttcctgct gttctgcggt
    22021 gcgtgaagtg agcgtcaggc gggaaaccga actgatactc gccagcgcct caccgcgaaa
    22081 gcccaggctg ataatggctt cgagatcgtc cagagaggcg attttactgg tggcatgacg
    22141 cgccagcgcc agcgccagct cgtctttttt gataccgcag ccgttatcac gaatgcggat
    22201 aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc gtcgcacctg catcgaggct
    22261 gttttccacc agttctttga ctaccgacgc aggtcgctcg accacctcac ctgcggcaat
    22321 ctggttcgcc agttgtggcg gtaagacctg aattggcatc aatcgtcctt agtttggcag
    22381 cgtacgatct ggcgtcgtca ccgtactggc cgtttgtgcc gttgccccct gcggcgcaga
    22441 ttgcatcgga tgcgcaagga aataattgcg caggcctttg taaatggctt ctgccagctg
    22501 ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg ttgttgctga taaaaccggt
    22561 ttcgaccagt actgatggga tatccggcga acgcagaacg ccaaggctgg catgttctgg
    22621 ccgacgttta tgtatttcgc caatgcgttg caactgactg atcatactgg tcgctacatc
    22681 ataccctacc cgctgggaat gaccgaactg taaatccagc accgcctggc ttaaataggg
    22741 gtcagactga ctgttcgcca gcacatcacc cgccccgccc agcagctccg actgtttctc
    22801 gtgctgctcc agccagctgg ccatttcgct gttggcgcga cggttagaga gcacccatac
    22861 ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg tgaatcgaca cgaggaaatt
    22921 ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc gagataaagt aatccccgtc
    22981 acgggttaaa acgcctttaa acatcggatc gtcattgagc aaagtacgca atttacgcgc
    23041 gatggcgatg gtgacatttt tctcccgcgt accaccgggg ccgatagcgc cagggtcctg
    23101 accgccgtgt ccggcatcaa tagcgatgat aattttatcg ccagtgttag ccgtcgcgcg
    23161 cgctgccgga cgcgttaccg tattactgct gataacaccc gtagtgcggt tactttccgt
    23221 tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg acaaccgtag gcgtttcaac
    23281 gcgtttcgca accacgggcg gcggaggagg cggtggcggc gcatcggcgt taatcgtaaa
    23341 gacgacagtg taattgctgc cattctgccg cttcaccgct tcggttttac cattttcggt
    23401 aagatcaacc accagccgta gcgtttgtgc atctttaggc gttccggagc gaatcgcctt
    23461 caccagatta ttaccgctga acaacaacgg cagtccctga atcacgcccg tttgtttgat
    23521 atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca taatcaggat cgccaataaa
    23581 actcaacgtt atccgcgcct gttggttgcc gttagaaacc tgaatatcag agagcgtcgc
    23641 ggcacccacc tgcgcgcaca gcagcagcaa cgtcgctacc aaccaatttc tgatgcgata
    23701 catcatcccg ccacctttca aaggttaacc ggctaaacgc gccagcaaca attcacccgc
    23761 agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc tgataatcaa tgtgtatttc
    23821 gacatccggg tcaggaagaa cacctgtacc ttgttgtggc cactccacca ggcagatggc
    23881 atcgttggca aaataatcgc ggatccccat aaactccagc tcctcgggat cggcaaggcg
    23941 gtacaaatca aagtgataaa ccattaagtt gtcgagcgta tagggttcga ccagcgtata
    24001 ggtcgggctt ttgacattac cctgatgacc caaagcctgc aaaaagcccc ggctaaaggt
    24061 ggttttacct gcgcctaaat cgccatacag atagatgacg gttgcaccat cgcaggcttt
    24121 ggctacccgc tcgcccaggt ctaaggttgc ctgctcatca gggagcggaa ttactcgatt
    24181 catcatggtt tttttcagtc acttccgggt taacaatacg ctgtagcgtg gaaaagagat
    24241 cggttgccag catcccgcgc gttccaaaac gcgccgccag tacgtcagct gccgcaccgt
    24301 gcgcgacaca gcctgcacag gctgcatcat acggcgacag tttttgccca agcaatgcgc
    24361 caataatacc agagagcaca tcgcccatgc cgccgcttgc catgcctgca tttccggcat
    24421 caataatgcc taaagcgtca ggatgggcgg cgaccacggt tccggcacct ttcagcaccg
    24481 ctacgccgcc ataacgttgt accagacgtt tggcgcaatg taagcggtca ctttcaattt
    24541 cagcgacgga acagcctaac aaccgtgcgg cctcgccagg atgcggcgtg atcacgcgat
    24601 tgtgacgctt atcgggattg attgccagca ggttcaatgc atcggcatcc cacaacatcg
    24661 gtttgcgaaa attctcaact ttttgcaggg cttttttccc ccactcttgc tggcccagac
    24721 cgggaccaat caccaccaca tcggcccatt ccaggctttc ggtaagagag tccatcgtca
    24781 gttcatgcac catcaattct ggtcgtgcag tcagcagcgg cgcaatgttc tcactgcggg
    24841 tcagtactcg gactaaacca gcaccagcac gtagcgccgc ttcccccgtc atacgaatag
    24901 cccccgccgt gccgtgatcg ccaccgataa tcaccagccg cccgtgatcg cctttatgcg
    24961 aagtcgggcg acgcggtttc agccagtgag aaagttgttc tgccgaaaac cgctgaattt
    25021 tcgtctcctg acctgccagc caactatcca accccagtga gtcaaaatgc agttgtccgg
    25081 taacatcccg cgcttttcca gtgagcaagc ctggtttcag cgcaataaaa gtgatggtgt
    25141 gatcggcgtt gatcactgcg cctggcgtag cgccggtttc agccagcagg ccggaaggga
    25201 tatcaaccgc cacaatcggc gcaggatggg aattagcgtg gtcgattaac tggctaatgg
    25261 attcgcgggg cgcttgctgc aagccggtac cgagcatcgc atcaacaatc agatctaccg
    25321 attcgggcca gacaatattc gaagcatgga tctcgccacc cgcgtttaac catgcttcgc
    25381 gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc ctgggccaac aacgtgacct
    25441 caatgccgac cgctttggcc agtcgcgcga ccacgtagcc atcgccgccg ttattaccat
    25501 gaccgcacaa caccagccag tggcgggcgt caggatacgc cgaacgacac acctggaatg
    25561 cggcctcgcc agcgcgaagc atcagctcat agagtgtgag ccccagcgca tctgccgcct
    25621 cgcgttctcc gcggcggata tcgtcggcgt gccagacggt gtgtggtata cttacggggt
    25681 ttttcttcat tgtatggtcc gtcatgtcag agcccctcga tctcaatcag ttagcgcaaa
    25741 aaattaaaca gtgggggctg gaactgggct ttcagcaggt aggtattacc gataccgatc
    25801 tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa acaataccac ggcgaaatgg
    25861 actggatggc acgtcacggt atgctgcgcg ctcgccccca tgagttattg cccggtacgc
    25921 tgcgcgtgat cagcgtgcgg atgaattacc ttcctgctaa cgccgcattt gccagcacgc
    25981 tgaaaaaacc caaactcggc tatgtcagcc gttatgcgct gggccgtgac tatcacaaac
    26041 ttctgcgcaa ccgactcaaa aagctgggcg agatgattca gcaacattgt gtttcgctga
    26101 attttagacc gtttgtcgat tctgcgccta ttctcgagcg cccgttagct gaaaaagctg
    26161 ggctcggctg gacaggtaag cactcactta tcctcaatcg cgaggccggt tcgttcttct
    26221 ttttaggcga attgctggtc gatattcctc tgcccgtgga tcaaccagtc gaggaaggat
    26281 gtggtaaatg cgtggcctgt atgacgattt gcccgaccgg tgccatcgtc gagccatata
    26341 ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga actggaaggg gcgatcccgg
    26401 aagagttgcg accgttaatg ggaaaccgta tttacggttg cgatgactgc cagcttatct
    26461 gcccgtggaa tcgctattca caactcacta cagaagacga tttcagcccg cgtaagccgc
    26521 tacacgcacc ggaactcatt gagttattcg cctggagcga agagaagttt ttaaaagtca
    26581 cggaaggttc ggcgattcgc cgtataggtc acctgcgctg gctgcgtaat atcgccgtgg
    26641 cattaggcaa tgcgccctgg gatgaaacga ttctggcggc gctggagagt cgcaaaggtg
    26701 agcacccact tcttgatgag cacatagcgt gggcgatggc gcagcaaatc gagaggcgaa
    26761 atgcgtgcat agtcgaagtg caattgccga aaaaacagcg tctggttcgg gtgattgaaa
    26821 aagggttacc gcgtgacgcc tgattcatcc acagcttgtg aataaaaata aaaacgcatt
    26881 gcaattcaag acgaataaaa tcgtcaagtg atcgactcga caaaacggaa tcttaatttt
    26941 taacttacct atcaataggt taaaaaaata ctattcaccg tgcgaagttt ctttgaaatt
    27001 aagattacag cctgaggctg tggataactc tgtttacaag agtttgtcag agacaacaaa
    27061 aaccagcaat aacgcatcgc gtcgctgtgg atattttatt gagagaaga
//
LOCUS       DAZFFG010000065        27027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFFG010000065 DAZFFG010000000
VERSION     DAZFFG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.065"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..497
                     /locus_tag="ACHJ7J_002966"
     CDS             <1..497
                     /locus_tag="ACHJ7J_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO5001168.1"
                     /translation="YNTNCTGANCSNILFDYAWLDNTIFIKTLFKNTCFYNVRAKNVY
                     LEGAYLNNDNIVKQANNSTEKQANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDS
                     TDKQANDNIDKQVNDSTDKQAKNSTKQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTY
                     IVEE"
     gene            766..1608
                     /locus_tag="ACHJ7J_002967"
     CDS             766..1608
                     /locus_tag="ACHJ7J_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO5001169.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSPQDHTF
                     WFFKEKNPIDVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1676..2854
                     /gene="yjiJ"
                     /locus_tag="ACHJ7J_002968"
     CDS             1676..2854
                     /gene="yjiJ"
                     /locus_tag="ACHJ7J_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5001170.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSDKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            3065..8305
                     /gene="iatD"
                     /locus_tag="ACHJ7J_002969"
     CDS             3065..8305
                     /gene="iatD"
                     /locus_tag="ACHJ7J_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
                     /protein_id="HFO5001171.1"
                     /translation="MASTNAHQIKNNDQNSLCGLGDKIRHLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSAPPLLENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTSQKSHEQQNAIPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTARISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTGLGWRHFTSSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDNDFEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSLQTKYALKGYNIEAAALEAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYIQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDESNQPVAYQKEELVKAVT
                     VENSKPGTTIVWHEEQPGVYTANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLINHQVTFKNEKGSAEFVEPPQQ
                     NTDGYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTAIVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEVDKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNIVTLTASVKDV
                     YGHPLPDEDVKFTLPASMTGNFTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAYQQVTFIGDTNSAQLQPLTASLNSIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSGTEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGENITLRILVQDAFNNVIAGQRVRLSAQPTANITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARGELMPGQIITFSVTPEGATLSNTGEVLTDQSGQAKVTLTSDKVNVYTVTA
                     IMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTITSRVEDDYGFPVE
                     GVDVSYTLDTKGRPVVNIPTTRTDQSGQVTATITSTLAETLTVNVQVPGTANQSAAIT
                     LIAGTADESKSILKSDVSTLMADYQHSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSMNLIRSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQGWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPGITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTPGAYWTSEPDDYSRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(8429..9013)
                     /gene="kptA"
                     /locus_tag="ACHJ7J_002970"
     CDS             complement(8429..9013)
                     /gene="kptA"
                     /locus_tag="ACHJ7J_002970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO5001172.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFNYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            9263..9946
                     /gene="yjiH"
                     /locus_tag="ACHJ7J_002971"
     CDS             9263..9946
                     /gene="yjiH"
                     /locus_tag="ACHJ7J_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO5001173.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9943..10404
                     /gene="yjiG"
                     /locus_tag="ACHJ7J_002972"
     CDS             9943..10404
                     /gene="yjiG"
                     /locus_tag="ACHJ7J_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO5001174.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10417..11589
                     /gene="iadA"
                     /locus_tag="ACHJ7J_002973"
     CDS             10417..11589
                     /gene="iadA"
                     /locus_tag="ACHJ7J_002973"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5001175.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIIPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11654..12565
                     /gene="hypT"
                     /locus_tag="ACHJ7J_002974"
                     /gene_synonym="qseD"
     CDS             11654..12565
                     /gene="hypT"
                     /locus_tag="ACHJ7J_002974"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO5001176.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12558..12950)
                     /gene="iraD"
                     /locus_tag="ACHJ7J_002975"
     CDS             complement(12558..12950)
                     /gene="iraD"
                     /locus_tag="ACHJ7J_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO5001177.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQLHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLALSLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12947..13030)
                     /gene="idlP"
                     /locus_tag="ACHJ7J_002976"
     CDS             complement(12947..13030)
                     /gene="idlP"
                     /locus_tag="ACHJ7J_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO5001178.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(13146..13289)
                     /gene="ytiD"
                     /locus_tag="ACHJ7J_002977"
     CDS             complement(13146..13289)
                     /gene="ytiD"
                     /locus_tag="ACHJ7J_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO5001179.1"
                     /translation="MADYAEINNFPPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            13622..14452
                     /gene="yjiC"
                     /locus_tag="ACHJ7J_002978"
     CDS             13622..14452
                     /gene="yjiC"
                     /locus_tag="ACHJ7J_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5001180.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNKLTREYEYARMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNISKQSWPFNYVTEICREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLLLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMEN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14593..15366)
                     /gene="uxuR"
                     /locus_tag="ACHJ7J_002979"
     CDS             complement(14593..15366)
                     /gene="uxuR"
                     /locus_tag="ACHJ7J_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO5001181.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15581..17041)
                     /gene="uxuB"
                     /locus_tag="ACHJ7J_002980"
     CDS             complement(15581..17041)
                     /gene="uxuB"
                     /locus_tag="ACHJ7J_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO5001182.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(17122..18306)
                     /gene="uxuA"
                     /locus_tag="ACHJ7J_002981"
     CDS             complement(17122..18306)
                     /gene="uxuA"
                     /locus_tag="ACHJ7J_002981"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO5001183.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLARCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18647..19990
                     /gene="gntP"
                     /locus_tag="ACHJ7J_002982"
     CDS             18647..19990
                     /gene="gntP"
                     /locus_tag="ACHJ7J_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO5001184.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLIGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(20165..21067)
                     /gene="fimH"
                     /locus_tag="ACHJ7J_002983"
     CDS             complement(20165..21067)
                     /gene="fimH"
                     /locus_tag="ACHJ7J_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5001185.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(21087..21590)
                     /gene="fimG"
                     /locus_tag="ACHJ7J_002984"
     CDS             complement(21087..21590)
                     /gene="fimG"
                     /locus_tag="ACHJ7J_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO5001186.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(21603..22133)
                     /gene="fimF"
                     /locus_tag="ACHJ7J_002985"
     CDS             complement(21603..22133)
                     /gene="fimF"
                     /locus_tag="ACHJ7J_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO5001187.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     ANFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNASSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(22143..24779)
                     /gene="fimD"
                     /locus_tag="ACHJ7J_002986"
     CDS             complement(22143..24779)
                     /gene="fimD"
                     /locus_tag="ACHJ7J_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO5001188.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTSWSYNSSDRSSGSKNKWQHINTWLERDLIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(24846..25571)
                     /gene="fimC"
                     /locus_tag="ACHJ7J_002987"
     CDS             complement(24846..25571)
                     /gene="fimC"
                     /locus_tag="ACHJ7J_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5001189.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(25608..26147)
                     /gene="fimI"
                     /locus_tag="ACHJ7J_002988"
     CDS             complement(25608..26147)
                     /gene="fimI"
                     /locus_tag="ACHJ7J_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5001190.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(26212..26760)
                     /gene="fimA"
                     /locus_tag="ACHJ7J_002989"
     CDS             complement(26212..26760)
                     /gene="fimA"
                     /locus_tag="ACHJ7J_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5001191.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     ATHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
BASE COUNT         6868 a         6823 c         6484 g         6852 t
ORIGIN      
        1 tctacaatac taattgtact ggtgcaaatt gttcgaatat cctatttgac tacgcatggc
       61 ttgacaatac aatatttata aaaacgcttt ttaaaaatac ctgtttttac aatgtcagag
      121 cgaaaaatgt ctatcttgag ggagcatatc tgaacaatga taatatcgtg aaacaagcca
      181 ataacagtac cgagaaacaa gccaatgaca gtacggtgca acaatccatt gacagtacgg
      241 tgcaacaagc caatgacagt accgataaac aagccaatga taatatcgat aaacaggtca
      301 atgacagtac cgataaacaa gccaatgaca atatcgataa acaggtcaat gacagtaccg
      361 ataaacaagc caagaacagt accaagcaac aggacagtaa cagttttaat caagcccgtt
      421 taaagaaaga agtgaatagg agattttcca ttccgggttt aacgtcttat cagccaacgt
      481 atatagttga agaatagttc aataatcacc caaacagagc aggtatggca gaggatataa
      541 attaacattt aaacgttaac cgcctcatct atcattcatg gatgaggcgc atttatcagt
      601 cgatgtaagc tgaaaatgtt ctgccatcca gagacaaccg ccgcccccct gcggcgttta
      661 ctatttcatc gcccgcgtta agaattcatt aagcttcatc cgtatactcc ttatctgtcg
      721 cactgaattt gacgagaaaa aaaagcaata ataaggagca ataagttgaa aaagcgcatc
      781 acctttatcg tttttctttg cgccatcgtg gcggcaagcc tgttttttgt gcaatcctgc
      841 gtccgtaaaa gccagcatgt cgcagggttt cagaattatc aggccactat cgatggtaaa
      901 gagattacag gagtaactaa aaatatctcc tcactgactt ggtcagcgca gagcaatacc
      961 ttatttagca ctatcaacaa acctgcaacc atcgttgaaa tgacgaccga gggcgacttg
     1021 atccgcacca ttccgctcga ttttgttaag gatctcgaga cgattgaata cattggtgat
     1081 aataaatttg tcatcagtga tgaacgtgac tacgccattt atgtgatttc acttaacgct
     1141 gactcggaag tgagcatcct caaaaaaatt aaaatcccat tgcaggaaac gcctactaac
     1201 tgtggctttg aaggactggc ttactcccct caggaccata ccttctggtt ttttaaagag
     1261 aaaaatccga tagatgtcta caaagtaacc gggctattgc gtagcgatga gttgcatatc
     1321 agcaaagata aaacgttgca gcgtcagttt actttagacg atgtttctgg cgcggaattt
     1381 aacccacaga aaaatacgtt gctggtgttg tcacatgagt cacgcgccct gcaggaagtg
     1441 accgtgagag gtgacgtgat cggcgaaatg tcgttaacca aaggtaagta tggcctttca
     1501 cacaacatca agcaggcgga agggatagcg atggatgata gcggcaacat ctatatcgtt
     1561 ggcgaaccga atctttttta tcgctttacg tcgacaaaat cacgataaca gtctggcagg
     1621 ctctcctttt ttgttattaa cttgttaact caaaataata caaaccaggc agaacatgaa
     1681 ttcgacatcg catcccgtag aacgtttttc tttcagcacc gcgttattcg ggatgctggt
     1741 tctgacctta ggtatgggtt taggccgctt tctctatacg ccgatgctgc cagtcatgct
     1801 ggcggaaggc gagttttcat ttagcgaact ctcatggatc gccagtggta actatgccgg
     1861 gtatctggcg gggagcctgc tgttttcatt cggcgcgttt catttaccct cacgcctgcg
     1921 cccgttcctg ttagcttccg ccctcgcaac cggattatta atcctcgcga tggcgtggct
     1981 gccgccgttt cttctggttt tcatcattcg ctttctggcg ggagtcgcca gcgccgggat
     2041 gttgattttc ggctcaacgc tcatcatgca acatactcgc catccttttg tccttgcggc
     2101 gctattttct ggtgttggcg tcggcatcgc tctgggtaat gaatatgtgc tggcaggcct
     2161 gcattttgcc ctctcttcac aaacgttgtg gcaaggtgcc ggagcacttt ctgccattat
     2221 attgcttgct ctggcgctgc tcatcccgtc ggataaacac gttatcccgc cagcgccatt
     2281 ggcaaaaatc gcgcaacaac ccatgagctg gtggctactg gcgattctgt atggcctggc
     2341 gggttttggt tatatcatcg tcgccacata cctgccgctc atggcgaaag acgcgggcca
     2401 gcctgtgctg acggctcacc tctggacact ggttggcttg tcgattgtcc caggttgctt
     2461 tggctggctg tgggcagcca aacggtgggg agcattacct tgcctgaccg cgaatttgct
     2521 ggtgcaggcg atctgcgtgc tgttaaccct cgccagcagc tctcctttat tactcatcat
     2581 cagcagtatt ggttttggcg gcacctttat gggaacgacc tcgctggtga tgaccatcgc
     2641 ccgccagctt agcgtgccgg gaaatcttaa ccttttgggc tttgtgacac tcatttatgg
     2701 tatcgggcaa attcttggcc cggcgctgac cagtatgctc ggcaacggaa cgtcggcgct
     2761 cgccagtgcc acactctgcg gcgcagcggc gctatttatc gcagcattaa tctgcgggat
     2821 gcaaatattc aaattgcata cgaattattc ttaagtaatt taacgaattt aacttttcag
     2881 tacatcacgt aaaataatta aaacatcctt tttctttgga ggtactcagc caccgccggt
     2941 attattcctg gtttagcaat tccacggcta attcattatt tctggtcata ttatagtcag
     3001 gatcgcatta tatctccgtg ctagaaaaaa caagtttaat tctgtttttt cacggaaggt
     3061 cactatggct tcgacaaatg cgcatcaaat aaaaaataat gatcaaaact ctttatgtgg
     3121 tctgggtgac aaaatacgcc acctgaccgc cggagtttgt ttatttacac aaattttttt
     3181 ccccgtcatg gcgacggcac aaaatgtggt acatgctaaa cctcagacga cagtttcatc
     3241 tgccccccct ctactagaaa ataatacggt gccttacacg cttggtgcgc tggaatcagc
     3301 gcaaagcgtt gctgatcgat tcggtatttc actggaggag cttcgtcgtc ttaatcagtt
     3361 ccgtactttt gctcgcggct ttgataacgt gcgccagggt gaggaactgg atgttccggc
     3421 aacaacctcg cagaaaagcc atgagcaaca aaatgccata ccgcctgcga atggcgaaaa
     3481 cacgctggag aatcaaatag ccagcacctc gcagcgagtt ggcactctgc tttcacaaga
     3541 tatgaatagt gagcaggcca gcggcatggc gcgtggttgg gcgtcttcag aagcctctgg
     3601 cgcgatgact gattggttaa ataactttgg tactgcgaga atctctctgg gtgtggatga
     3661 agattttagc ctgaaaaatt cgcaattcga cttcctgcat ccgtggtatg acacacccga
     3721 ttatctgctc ttcagccagc atacccttca ccgaacagac gatcgtaccc agatcaacac
     3781 cggtttgggc tggcgtcatt tcacctccag ctggatgtca ggcatcaacc tgttttttga
     3841 ccacgacctg agccgctatc actcccgcgc agggcttggc gcagaatact ggcgtgatta
     3901 tctgaagttg agcagcaacg cttatatcgg cctgaccggc tggcgtagcg caccagaatt
     3961 ggataacgac ttcgaagccc gcccggccaa cggctgggat ttacgcgcgg aaggctggtt
     4021 acctgcctgg ccacaactgg ggggaaaact ggtctatgaa caatactatg gcgatgaagt
     4081 ggcgctgttt gacaagaatg atcgtcaaag taacccccat gctattaccg caggcctcaa
     4141 ctataccccc ttcccgcttc tgactctcag tgcggaacag cgtcagggga agcaaggtga
     4201 aaatgacaca cgttttgccg ttgatctgac ctggcaaccc agcagttcaa tgcagaaaca
     4261 gcttaacccg gacgaagtgg ccggacggcg cagtctggcc ggtagtcgtt atgacctgat
     4321 tgatcgcaac aacaacatcg ttctggaata ccgcaagaaa gagctgattc gcctgagtct
     4381 gctggatcca gtgaaaggga agtctggaga aataaaaccg ctggtttcct cgctacagac
     4441 caaatatgcc ctgaaaggct ataacatcga agccgctgcg ctggaagctg ccggaggtaa
     4501 agtcagcacg tctggaaaag atatcacggt cacgctgcca ggttaccgct tcactaacac
     4561 cccagaaacc gataatacat ggtcgataga cgttaccgcc gaggatgtaa aaggtaacct
     4621 gtcacggcat gaacaaagca tggtagttat tcaggctccg acattaagcc agaaagattc
     4681 tctgttatcc gtcaatccgc taaccgtggc tgcagataaa aaatcgacga ccacattgac
     4741 cgttactgcg cacgattccg acggaactcc ggtgccgggg ctggcgctgc aaacccgcag
     4801 tgaaggcgtt caggatatca ccctgtctga ctggacagat aacggtgatg gtagttacat
     4861 acagatgctg accgccggaa cgacatcagg ttcagtaaca ctgacgccgc aaattaacgg
     4921 tgagagtgcg gtaaaagaat ccatcgtcgt taatatcgtc cctgttgtct catcccgcga
     4981 ccattcatca ataacaattg ataacgtatc gtattatgcc ggagacgaca tcaaggttag
     5041 ggtggagctg aaagacgaaa gtaatcaacc ggttgcatat caaaaagagg aattggtaaa
     5101 agccgttact gtcgaaaaca gcaaacctgg caccacgatt gtctggcacg aagagcagcc
     5161 gggcgtttat accgcgaatt atccggccca taagcaaggg actgcactaa gggcacaact
     5221 tagccttcac aactggaatg ctccactgca atcgcatatt tataacattg aggcaaacca
     5281 gaataaggct cgcgtcgcca cattatcagc gacaaataat gacgtttacg ccgataaaaa
     5341 gacatttaat accctcacta tcaacgtcac tgatgagagt gataatcccc tgataaatca
     5401 tcaggtcacc tttaagaatg aaaaaggaag cgcggaattt gtcgaaccgc cgcagcaaaa
     5461 tacggatgga tacggtgttg ccacaatcaa catggtaagt caggttgcgg aagaaaatac
     5521 gattagcgcc acgctgccaa atggtttctc acaacggata attgcgaaat tcgttagcga
     5581 ttcgagtacg ccaaaattca aacaactggt tgccgatcca gataccatta ttgctggcaa
     5641 cagccagggc agtactctga ccgccatcgt cacagacttt cataacaacc cgttaaaaga
     5701 tatgaaagtg aattttgtgg cacctggtgg ctcgcaactg gacaacacga ccgccacaac
     5761 agaccagtcc ggtattgtgc gggtgcacct gaccagttca aaagctggta gctattccgt
     5821 cgatgcctcg cttgaggtgg ataaaaatat tcaccagtcg gtcacgatca ccgtggtccc
     5881 aaacagggaa caatcggtaa tgaccttgaa tgccgggtcg ggcagtgcga tcgctaacaa
     5941 tacaaatatc gttaccctga ctgccagtgt gaaagatgtt tatggacacc cgttgccgga
     6001 tgaggatgtg aaatttacct tgccagcctc catgaccggg aacttcacgc taagtagtga
     6061 aaccgcccgc accgatgcaa acggtgatgc cgtggtcaca ttgcgaggca caaaagcggg
     6121 tgagtttaca gttacggcga cgctgaccag aaataatacc gttgcttatc agcaagtcac
     6181 ttttattggg gatacaaaca gtgcgcagct ccagccgctg actgcctcat taaattccat
     6241 tgttgcgggt aacagtacgg ggagtaccct gacggcaacg atcctggacg cttaccaaaa
     6301 tccgcttaaa gaccagttgg tcactttcca gagtaacgat gtcactctaa gcggaacaga
     6361 agtcaccacc aatacgctgg gtcaggcgac ggtaacaatg accagcaata ttgccggaca
     6421 acataacgtc gtggtgagcc ggaaagcgca agcctccgat aataaaacgt ttagtttatc
     6481 agtgctgccg gatgaaagtt cggcgaaggt aataagtata accggagccg aaaaaacgat
     6541 aacggtgggc gaaaacatca cgctacggat actcgtccag gacgcgttta acaatgtaat
     6601 cgcgggtcaa cgcgtcagat taagtgcgca gccaacagct aacattacga taggcgatac
     6661 ggcttacacc gataataacg gttatgcgta cgttaacctt ctcagcaccc aacctggggt
     6721 ttatcaggtg acggcaacgc tggacaataa cagtagtagt aaggttgacg tgaatgtggc
     6781 aaatggcaaa ctcgagttaa catcatcgaa accagaaacc acggtccata atagtgaggg
     6841 tattacgctt accgcaacgg cgagaaatgc gcggggtgaa ttgatgccag ggcaaattat
     6901 cacctttagc gtaacgcctg aaggtgcaac gctaagcaat acaggggaag tccttactga
     6961 ccagtcaggt caggccaaag tgacgctgac cagtgacaaa gtgaatgtct ataccgttac
     7021 ggccataatg ggcaaagatg ttcccgttca gagccaggta acggttgcgg ttaaggcaga
     7081 tgctaaaacg gcacatgttg tgagcgtcgt ggcttctcct gacaccatca ccgccgacgg
     7141 catcgatagc agcaccatca cttcacgagt agaagatgat tacggattcc cggttgaagg
     7201 tgtcgatgtt agttatacct tagacaccaa aggtcgcccg gtagttaata ttccaactac
     7261 gcgtaccgat caatccgggc aagtcacggc gacaataacc agtacattgg cagaaacctt
     7321 aacagtcaat gtgcaagttc ctggtacagc caaccaatcc gcagctatta ccttgattgc
     7381 cggcacggcg gatgaaagta agtcaatttt gaaatccgac gttagcactc tgatggctga
     7441 ctaccagcat agcgcgaaac ttacgctaac attgcaagac aagtacggta acccgatagt
     7501 gacgtctgat catctggaat ttgtccagtc aggtcccttc gtgaactttc tcaagttgag
     7561 cgatattgat tacagccaaa gaaattatgg cgagtacacc gtgactgtca ctggcggaaa
     7621 agagggaaca gcgactctca ttcccatgct gaacggggtt catcaggcaa acttaagcgt
     7681 atcgatgaat ctcatccgct cgataaaaga aatgtccggt catgtcactg caaacaacca
     7741 taccttctcc acggctaaat tcccgagcga aggttttgca ggagcgtatt acacactcaa
     7801 caatgataac tttgaagcgg gtaaaaccgt tgatgattat atgttttcaa gttcacaggg
     7861 ttgggtgtct gtcgatgctt caggtaaagt ttctttcgca aatatcggcg atcaaacgtc
     7921 agtcacaata agcgctgttc cccgacaagg aggtacaacc taccagacct taattaagct
     7981 gaaaggctgg tgggtgaata atggaaatca taccaatatc tggctagctg ccaatgcgct
     8041 ctgtcatgct aaaaatgatg gatataatct tcctggcatc acacatttga cgtctggcga
     8101 aaacaaacgc acgcagggat cactgtatgg tgaatggggg aacgttggag cgttttccag
     8161 taattcgcaa tttacaccgg gagcttactg gacaagtgaa cctgatgatt acagtcggca
     8221 ctactatgtg cagatgctaa ccggtatgac cggaagcgac gctgattcca gcccccaact
     8281 gaccgcctgc cgtaaatcac tttaatttca gcagttgtta ttaaaggata cccttttacg
     8341 agggtatcct ttcagactga taacaaacgc tacattgccg gatgtgctgc gcttatcagg
     8401 cctatatagc ccctgcaata tattgaattt attgtttttg taccccagtc ggcataaacg
     8461 accactcaag aaactcaaca gcaaccgttg atgtcagcca gaccccgttt tccgcttgcc
     8521 agaaagggat gcctcgtttc gccatctcct gtgctttgac ggttaagata accggcgatc
     8581 cgtgtcttgc tcccactttg cgtgccgttg cttcatcagc agagagatgc acgtaatgac
     8641 gatcccctgc aatcagcccc tgttttttta tctcgtcaag gaaacggctt gccgtaccgt
     8701 ggtagagaaa ctgtggcggt gttttttcgg caaaggaaat agcgacctgc gaagtcgaat
     8761 gcccctgaac cgcgcggatg cacctaccat cactggaata attaaaacgc tttttatcgc
     8821 tggtggcgac cacggtatcc agcaatgcac gggttaaccg cttgcccgct ttttgggcac
     8881 agagtataag cttatcgata tccgcccatc cttcacggtc cagtacgata ccaatcgcct
     8941 ccggtttgtg tcggaggaca aaacttaaaa acttgctcgt atccgccagc tctttttcgt
     9001 tgtactttgc catcgcgttt tcgtccctgg attttctgtt tttatttact gatgtcattg
     9061 tcactgggtt tatgaatttt ctgcaactca atcctgcgca gagccttttc cacacagcgt
     9121 aattttaccg ctatgcaaaa aacgcatagc cagaaacata cttgcatttt ccccataccc
     9181 acatggctga taaggttttt ccgctccagg aattttcctg gctcaagcaa cacacaaaca
     9241 caaaaaaaca atggggattg ttatgacaca acaaggggat gctgtagccg gggagctggc
     9301 gacagaaaaa gtgggcatta aagggtatct ggcttttttt ctcaccataa tctttttttc
     9361 cggtgttttt tccggaacag acagctggtg gcgcgttttt gatttttccg tgctgaacgg
     9421 atcgtttggg caactgcctg gtgcgaatgg agccaccacc tcatttcgcg gggcgggtgg
     9481 cgcgggggct aaagatggct ttctctttgc gctggagctg gcaccgtcag tcattctctc
     9541 tctggggatt atttcaatta ccgatggtct tggtggatta cgcgccgcgc agcagttaat
     9601 gacacctgtg ctaaaaccgt tgttgggcat tccagggatc tgctcgctgg cgctgatcgc
     9661 taacttgcaa aacactgatg ccgccgccgg tatgaccaaa gagctggcgc aggaaggtga
     9721 aattaccgaa cgcgataaag tgattttcgc cgcatatcaa accagcggta gcgccatcat
     9781 caccaactac ttctcttccg gcgtcgcggt gttcgccttt ctgggcacgt ccgtaatcgt
     9841 acctttagcc gtcatcctgg tgtttaaatt tgtcggagcc aacattttgc gcgtctggct
     9901 taatttcgaa gaacgtcgca acccaacgca aggagcgcaa gcatgacaac tcaggtccgc
     9961 aaaaacgtca tggatatgtt tatcgatggc gcgcgccgtg gctttactat cgccacgacc
    10021 aacctgctgc caaacgtggt gatggcgttt gtcatcattc aggcgctgaa aattaccggc
    10081 ctgctcgact gggtggggca tatctgtgaa cccgtaatgg cgctgtgggg attacccggt
    10141 gaagcggcaa ccgtcctttt agccgcatta atgagtatgg gcggcgcagt cggtgttgcc
    10201 gccagtctgg caaccgctgg cgcattaacc ggtcacgatg tcaccgtttt actgcccgcg
    10261 atgtatttaa tgggcaaccc ggtgcaaaat gtcggtcgct gcctggggac agccgaagtg
    10321 aatgccaaat attacccgca tatcattacg gtctgcgtga ttaacgcatt gctgtcgatc
    10381 tgggttatgc agcttattgt ttaacaagga gttaccatga ttgattatac cgcagccggt
    10441 tttaccctgc tgcagggagc gcatttgtat gcgccggaag atcggggaat ttgcgatgtc
    10501 ctcgtcgcta acggcaaaat tatcgccgtt gccagtaata tcccttctga cattataccg
    10561 gactgcacgg ttgtcgatct cagtgggcag atcctctgcc caggttttat tgatcaacac
    10621 gtccatttga ttggcggtgg cggcgaagca ggtcccacga cgcgcacgcc ggaagtggcg
    10681 ctaagtcgcc tgacggaagc gggcgtcacg tcagtggttg gtctgctggg caccgactct
    10741 atctctcgcc acccggaatc cctgctcgcc aagacccgtg cgctcaatga agaaggcatc
    10801 agcgcctgga tgctgaccgg cgcttatcat gtcccttccc gcactattac gggttccgtg
    10861 gaaaaagacg tggcgattat cgatcgtgtg attggcgtga aatgcgccat ctctgatcac
    10921 cgttctgccg caccggacgt ttatcacctg gctaatatgg cggcagaatc ccgcgttggc
    10981 ggtttgctcg gcagtaaacc tggcgtcacc gtgttccaca tgggcgacag taaaaaggcg
    11041 ttacagccta tctatgacct gctggaaaac tgcgatgtgc cgatcagcaa gctgctgccg
    11101 acccacgtta accgcaacgt accgttgttt gagcaggcgc tggagttcgc gcgcaaaggc
    11161 ggcaccatcg atatcaccag cagcattgac gaaccggtcg cccctgccga aggtattgcc
    11221 cgcgccgttc aggcgggtat tccgctggca cgcgtcaccc tcagctccga cggcaacggt
    11281 agccagccgt tcttcgatga tgaagggaat ttaacccata tcggcgttgc aggttttgaa
    11341 acgttgctgg aaaccgtgca ggtgctggtt aaagactatg atttcagtat cagcgatgcc
    11401 ctgcgcccgc tcaccagtag cgtagccggt ttccttaacc tgagcgggaa aggcgaaatt
    11461 ctgccaggca atgacgcaga cttactggtc atgacgccag aattgcgcat tgagcaggta
    11521 tacgctcgcg gcaaactgat ggtcaaagac ggcaaagcct gcgtgaaagg aacgtttgaa
    11581 acggcataaa cggttgtcag ccgccctaat ggacgtagaa tgcccgcaag ggcggctgac
    11641 agagtaaaac gtaatggatg actgtggtgc ggttttgcat aatattgaaa ctaaatggct
    11701 ttatgatttt ctgaccctgg aaaaatgccg caatttttcc caggcggcag ttagtcgcaa
    11761 cgtctcgcaa ccggcattca gccgccgcat ccgtgcactg gaacaggcga ttggcgttga
    11821 attgtttaac cgccaggtga cgccgctgca actctcagaa caagggaaaa tcttccattc
    11881 gcagatccgc catctgttgc aacagttaga aagtaacctt gcagagctgc gtggcggcag
    11941 cgattacgcg caacgtaaaa tcaagatagc cgctgcacac tctctttccc tcggtctgtt
    12001 accgtccatt atcagccaga tgccgccgct ctttacctgg gcaattgaag ccattgatgt
    12061 cgatgaagcg gtcgataaac tgcgtgaagg gcaaagtgac tgtatttttt cctttcacga
    12121 cgaagatttg ctggaagcgc cgtttgacca cattcgctta tttgaatctc aattattccc
    12181 tgtctgcgcc agtgacgaac acggagaagc actttttaac ctcgcgcagc cacactttcc
    12241 gttactgaat tacagccgca actcctacat ggggcgattg attaatcgca ccctgacgcg
    12301 ccacagtgag ttaagtttca gcaccttttt tgtctcttcg atgagcgagc ttttaaagca
    12361 ggttgccctc gacggctgtg ggattgcctg gctgccggag tatgccatcc aacaagaaat
    12421 tcgcagcggg cagctcgttg tgctgaatcg ggacgaactg gtgatcccta ttcaggccta
    12481 cgcataccgg atgaataccc gcatgaatcc cgttgccgaa cgcttctggc gtgaactgcg
    12541 cgagctggag attgtgctta gctgacattc tcaagcgtcg cactgcgcca accgccatta
    12601 tcccagacca gatcaaacgt tagcggttca tcgtcgtggt acagcattat ctcaaggctg
    12661 agcgccagcg tgtggcagta cgcttctttc aaattgacct gcaacgccgc aatccgtggc
    12721 tcatgaaata acagtgcaga acgcagctga cggcaatacc agctctggag atgtttgtcg
    12781 ctctggttat gcagttgcca ctcgggcatg cctgaaacaa gtaatgacgg taatgccgaa
    12841 tgaggagaat taaaaagtgt gaggagatcg ctgcataccg atttacgcag tgatgacgtt
    12901 ttattgcctg aaatttcagg taaaacagcc tgaagtgatt ggcgcatcat tttgcgcact
    12961 cctgacgttt agcaacatat gcggcttgcc ctgaacaccg ggcaccactg tattgatgct
    13021 cattttccat agccattccc ttttgttcat tacatccatg tcagtctgag acaggcctga
    13081 atcctgtcag caaagtataa aaccttactt gcctatgaat atctactgac ttttcaggaa
    13141 aaaagtcatg atttgataaa aatcatcaaa ctcaaaaaaa aaccgctagt atattctgaa
    13201 tattccgaat agttacgtag atgaaaatac ttgtcaccgc cgctacttaa ttccggtggg
    13261 aaattattta tttccgcata atcagccatg tattatttga aacaattagt ttgtaaataa
    13321 tataaattga taacatatca aatacatcaa aaataccatc cataggcatt attaatatag
    13381 acatcaatat agcaacatca aattccaatt agtaataaga tatgcttttt aattgcatga
    13441 atataatttc aaatgagcgt tttttgatac tttagagtgt ttcctaccct ttaatatcac
    13501 cgtgtagagt tgatatcact acaacagaaa catgcatgtc atttttttac ttctttttta
    13561 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
    13621 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
    13681 tcatcatcca aaagtcgaca ataagttaac acgtgaatat gaatatgcac gcatggatac
    13741 ggaaaacatc tatcttctgc ctcttactcg tggcaataac cataattatg atggaaaaag
    13801 cgttgtcgaa attagaaaac ttaatattag caaacagtcc tggccattta attatgtgac
    13861 agaaatatgc agagaattca atggcattac aacaacagga cgaatgctct atcgaaactt
    13921 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
    13981 cacagagtta gcagaaggat tattgctaaa tttatttatg aagagccctt tcgatcctgt
    14041 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
    14101 cggaaccacg ataggatata tttcaaccct tccagtttct caggcaaaaa gatggaccaa
    14161 tgagcagcca agaattgata tctatattga tcagataatc actgtctcag gggtagcaaa
    14221 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagatgg aaaatgaccc
    14281 aatcattggt atagaagcct atcctggtac cgcggagatc catgcaaaaa tgggatataa
    14341 agttatcccc ggtgatgaag atgcgccact caaacgcatg actctgcaac cgtcatctct
    14401 tccagaactg tttgaattaa aaaatggtga atggaattat attggtaaat agccactgag
    14461 aagaatgagt actaatattc aggttataca gatatttaaa caataccctg caatttacaa
    14521 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacgttta cccttgcgct
    14581 tattataata agtcaggcgt cgactttatc cagcggccat gaatcaaaca gatagccgtc
    14641 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
    14701 ctgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
    14761 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
    14821 gatccacatt ggattgtttt cccgccactg ccaggactga cggaataact ccaccagcat
    14881 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccgc tttcgctgct
    14941 acccggcgcg ctggaagcca gttcacgctc ttccagttgc aatgcctgac gcattttgac
    15001 gatatcttcg cgggtagcct gcagggcggc aaactcggcg atgttacttt ccagtaactg
    15061 acgcgcctgt aacagctcaa aaggacccgc atcattgcag acgttggcat ccggactgtc
    15121 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
    15181 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtccgcg tgacatcaag
    15241 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtacg gcgtctttat
    15301 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
    15361 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
    15421 cgccttatcc ggcctacaac gggcaatttt acggcccccg ttaatctcaa actataccat
    15481 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
    15541 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
    15601 cgcgcgcacc gcgttcgcac agctgctgat atgccgcagt caccgcgcca acaaagtcgg
    15661 cattctgcgg cagatcatcg gcaaaaatac cgctcaggcc cagcagcgct ttcacgcggt
    15721 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcgaccacgt
    15781 caatggcatt accctgctca tccacgccct gggtgtaacg catccagcca gccacgccca
    15841 gcgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccaacagac
    15901 gctgcggtaa cttctggctg ccgtccatcg caatctgcca ggtacggtga cgcagagacg
    15961 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtccgta ccttccggca
    16021 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
    16081 ggttagtcat ggtgtcggca atagtttcat agccgccgag gtaaccgagg tatgccagga
    16141 aagagtggct gccgttcagc atacgcagct tcatcatttc gaacggcaca acgtctgcaa
    16201 cgaactgtgc gcccacttta tcccagtccg ggcgaccatt aacgaagtta tcttcaatca
    16261 cccactgacg gaacggttcg caggcaatgg cgcatgggtc gtaaacaccc agctggtcag
    16321 caatttcctg taaggtttct ggcgtcgccg ccggaacgat acggtcaacc atggtgcacg
    16381 ggaaggtgac gttttcttca atccatgccg ccagttgcgg gtcacgcgcc tgagccagcc
    16441 ccagtaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
    16501 taaacgcttt caaccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
    16561 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
    16621 gttgaccgct tgccgcatca gcgcagtagc ctttttccgt gaccgttaga gagacaatcg
    16681 ccgtttgcgg acgcgccatc gcgttgagaa taccttcgca gccgtcgatt tccggatgca
    16741 gcgcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcta
    16801 cggtgtacag cagttgctgt tttttcaggt tttcgatcag cacgcggtcg ttgcctggca
    16861 tcaggttaac ttcgcagatg ccccagtcgc tgtcggtact ttccagcagg tgatgggtat
    16921 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
    16981 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
    17041 tcgtaaactc cagaatccgt cttcaaagag tgacggcaag gaagaaggaa ccggcacggc
    17101 gcgcagccat gccggtggat attaacggct aaagaaagcg cgctggatcg ccagttcgac
    17161 accgcgaact tcggccaggc ctttcagacg accaattgcg gagtaacctg ggttagtttt
    17221 cttcttcagg tcgtccagca tctgatgacc gtggtccggg cgcatcggga tcaggtcttc
    17281 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
    17341 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
    17401 ggtggaacgc agatgggtga agtaaatgcg cggaccgaac tgcttgatca tatcaaccag
    17461 atcgttgtca gcacgcacgc cgtaggaacc ggtgcacatg gtgaaaccgt tcgccatgct
    17521 atttacggta tcaaccatcc actgcatatc ttcaatggta gaaacaatgc gcggcaggcc
    17581 gaggattggg cgcggcggat cgtccgggtg aacagccata cgcacgccaa cttcttcggc
    17641 aaccggaata atcgccttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
    17701 atctttgtac agctccaggt gcttacggaa ctggtcgagg gtgtaccctt cttccgcgcc
    17761 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
    17821 gaagcgttca gctgcctgag caatttcttc ttcggtgtaa tccgcttccg cacctgggcg
    17881 tttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
    17941 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
    18001 gtagcacacg gtgcgaatac cgcaccgcgc caggttgcgc agagtctgct gatagttagc
    18061 gatccactgc tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
    18121 tacgacagac cacaccaggc ctgcatcttc aacgatcgcc ttgcgtttga ggatctcttc
    18181 tacggaccat acttcaccgt tcgggatatg gtgcaaagcg gtaaccacgc cagtcgcgcc
    18241 cgcctgacgg acatcagcta aagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
    18301 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgc ggaaccctgg
    18361 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
    18421 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaattctgaa attggttaac
    18481 cacatcacaa gaatttcact ctcatggttg ttgctgcaaa ttgcagcaaa gggtaatcga
    18541 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
    18601 tgtcgcccaa aaataacaac ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa
    18661 cattctctgg gtggtattcg gcattggtct gatgctggta ctgaatttga agttcaaaat
    18721 caattcaatg gtggctttgt tggtggcggc gctgtccgtc gggatgctgg cgggcatgga
    18781 tttgatgtcg ctgctgcaca ccatgaaagc gggcttcggc aacacgctgg gggaactggc
    18841 tatcatcgtg gtgttcggtg cggtcatcgg taaattgatg gtcgactccg gcgcggctca
    18901 ccagatagcg catacgttgc tggcgcgtct cggtctgcgc tatgtacagc tgtcagtgat
    18961 tatcatcggc ctgatttttg gtctggcgat gttctatgaa gtggccttta tcatgttagc
    19021 gccgctggtt attgtcattg ccgccgaagc taaaattccg ttcctgaaac tggcgatccc
    19081 ggcagtagca gctgccacta ccgcacattc actgttccca ccgcagccgg gtccggtggc
    19141 gctggtgaat gcttatggcg cagatatggg gatggtttat atctatggcg tactggtgac
    19201 gatcccaagt gtaatctgcg caggtctgat cctgccgaag ttcctcggca atcttgagcg
    19261 cccaacgcca tcattcctga aagcagatca accggtagat atgaacaatc tgccctcttt
    19321 cggcgtttcg attctggtgc cgctgatccc ggcgatcatt atgatctcta ccaccatcgc
    19381 caatatctgg ctggtaaaag atacccctgc ctgggaagtg gttaacttta tcggttcctc
    19441 gccgattgca atgtttattg cgatggtggt tgcattcgta ctctttggca ccgcacgtgg
    19501 tcatgacatg caatgggtga tgaacgcttt tgaaagtgcg gtgaaaagca ttgcaatggt
    19561 gattctgatc atcggtgcgg gtggcgtgct gaagcaaact atcatcgaca ccggcattgg
    19621 cgacaccatc ggcatgttga tgtcccacgg caatatctcg ccctacatta tggcatggct
    19681 gatcactgtg ctaattcgtc tggcgacggg tcagggtgtc gtttcggcga tgaccgccgc
    19741 cgggattatc agtgctgcaa tccttgatcc agcaactggt cagctgattg gcgtgaatcc
    19801 ggcgctgctg gtgctggcga cggctgcggg ttccaacacc ctcacccaca ttaatgatgc
    19861 ctcattctgg ctgttcaaag gttactttga cctgtcagta aaagacacgt tgaaaacctg
    19921 gggactgctg gagctggtca actccgtggt tgggctgatt attgtgttga ttattagcat
    19981 ggtagcgtaa aagaacacca gagcctgcca atggcaggct cagttcggat tgtcggcaaa
    20041 gtgcgctttg tttatgccag atgcgacgct gacgcgtctt atctggccta caaagggcta
    20101 acgtgcaggt ttttagcttc aggtaatatt tgcgtacctg cattagcaat gccctgtgat
    20161 ttctttattg ataaacaaaa gtcacgccaa taatcgattg cacattccct gcagtcacct
    20221 gcccgccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa gtccctactg
    20281 ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc
    20341 cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg
    20401 cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag
    20461 gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat
    20521 cgcagccgcc agtaggcacc accacatcat tattggcgta aatattccac acaaactgga
    20581 aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag
    20641 ctttaatcgc caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg
    20701 gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtggtc ggaaatggat
    20761 agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg
    20821 agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga
    20881 tttgcgtcga aagatctacg accaggtttt gccccacatt cacggcaggc gcaaggttta
    20941 cataaacatt agcgctgcca ccgccaatag ggatagcggt accattggcg gttttacagg
    21001 cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc
    21061 gtttcattac aattatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc
    21121 tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag
    21181 tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg
    21241 ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa
    21301 tatccggtac tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga
    21361 caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc agacatcaga
    21421 ctgaaagaat aaagatcgcc gagatcaacc gtggcattgg tggtggaaac tgtgcacggt
    21481 ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt
    21541 gcgagcgcca atattgccgc caataaatac ccacgtttgc gccatttcat gagcatctcc
    21601 agttactgat attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc
    21661 acctgtgtcg ccattagccg ggcgtaaaaa ttcaacgtat ttggtttacc cggcgtcagg
    21721 gtcgtccacg aaatcgcgga cgatgaagca ttaaggggta tctgattttg ctgctcattc
    21781 agaagctgta ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc aagcaggttg
    21841 gcattgtggc tatctgcaac accggtaaac ccaactttta cggcagaaac ggcattacca
    21901 cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat gttgttaaat
    21961 tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ctgattcagc ggccacacta
    22021 cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaaacgcga
    22081 cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc
    22141 ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt
    22201 tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg aacttttccc
    22261 gctagaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg
    22321 ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc
    22381 atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg
    22441 ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc
    22501 agcgccactc tgttttcccg atattcggtg gcataaggca gcacggcata accgcgccag
    22561 tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca
    22621 agtaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccagtacc
    22681 ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg
    22741 gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctattacca
    22801 tcgcctcccc cggcatagcc ggtttgcacg ctataactga ggttgttgtc ttccagcaac
    22861 gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga catgctgtag
    22921 ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga
    22981 atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt tttcgtcagg
    23041 ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctggaattgc
    23101 tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat
    23161 gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg
    23221 ttgtaatagt cggtgaattt cggcttaacc tgaataactc cgtcctgtgt ttcgatgttg
    23281 tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa
    23341 cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt atagagaaaa
    23401 cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata
    23461 tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt aaaagcacga
    23521 taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt
    23581 aatgtactct ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg gtattctcct
    23641 gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt
    23701 acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta
    23761 ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa
    23821 atgtcatacc cattttgttt gatagtgacc tgtgcagtac cacgagcaat accgtggatc
    23881 accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg
    23941 ccgcgaaagt taataccatc gaaaatatcg ccctgggtat aaccatcacc cagcgtcagc
    24001 cgggaacgta acggtattaa gtctcgctca agccaggtat tgatatgctg ccatttattt
    24061 ttgctacctg atgatctgtc gctactgtta taactccagc tggtattgtc gcgtaaacgc
    24121 cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca
    24181 atccgattct gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg
    24241 ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg
    24301 atcgtcaggt tgagtcgctg ctgaccaaca tctaaatgcg cagtagcgtc ctggaccatt
    24361 gtggttaatg gcacacaggc atcatccgcc agcagattca taccggcgac agaagccgta
    24421 ttcagcccca tactggcgag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg
    24481 tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg
    24541 acgcgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa atcggccaca
    24601 gcctggggat catccgctaa aaagcgcgga ttaaaataga gttcggcaga tgacaaaggt
    24661 gcctgtgcgg caaaagcaca ggcaacaaag agccggacaa aaaaaccagc caaacgatgc
    24721 ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt
    24781 attcctccgg tttaaaatca cccggcagtc aattcttttt attcaggcga aaaattcccc
    24841 ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc atttattgtt
    24901 cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga
    24961 ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac cgtcaggtaa
    25021 tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct
    25081 gcggcctgat cgggtggcaa cgctaattta gccgggcgat agtacagttt aatgcggctg
    25141 ataattgcga gctgtagcgt attctcagtc aattttgatt tatccattga cggaatcgct
    25201 ttaacgttca tccagaataa actttcccgg tcctgtggca attggttatt tgttgcatca
    25261 agaatacgca aggtattctc tttttttccc ttcatcgcaa acagaggagg cgtcacgata
    25321 aaacgaccat cctttacacc atcggcattt tccacccatg attgaattaa ataggtacta
    25381 ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg ataaattacg
    25441 cgagtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat tgccatgaac
    25501 atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac attgacgttt
    25561 ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta ctgataggtt
    25621 aaagagaacc aggcctgggc attggcgatg ccgccagtaa cccgacgccc ggtagcacga
    25681 tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca gtttgctgga
    25741 ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacgcc aatattggtg
    25801 gctatccctg gcccctctcc cacggaaagc acatccggat ttttaccatc cgcgacaccg
    25861 tgaaacgcca cacccacacg ttcactcacc accgtgctac attcccgtaa atgaataaca
    25921 aaaggcaccg gtgcgctatc ttcccctacc gcatgaaacc ggttactgct gatttgcccc
    25981 atattgaccg tcatttgttt atcaccggct tcaatccggc aagtttccgc aataatgacg
    26041 ccctgaaatt gcatatttcc gccgggcaac gtggtattcc atttatttcc ggccagagca
    26101 aacattggca gcaacgacgc taatagaaat aatctcttcc ttttcatcgt tatttttatc
    26161 gcacaagggt gggcatccct gcccgtaatg acgtccctga acctgggtag gttattgata
    26221 ctgaaccttg aaggtcgcat ccgcattagc tgcgccaggg gttgcgacac cggttgcata
    26281 ataacgcgcc tggaacggaa tggtgttagt accgttattc agggttgttt gctcactgaa
    26341 tgtcgcacca tccagcgtca gcgcagcacc cgttctgtcc aggatctgca caccaacgtt
    26401 tgttgcacta cctgcagcag aactctgcag agccagaacg ttggtatgag tcgcatcaat
    26461 cgccgtacct aaaaaggcaa cagcggcttt tgatgcaaca ttggtatcgc aatcattcag
    26521 ctgaatgtta aaaccgacag cagaactggt tgctccgtcc tgtgccagcg atgcggtacg
    26581 aacctgtcct aactgaacgg tttgatccac agagcctgca tcaactgcgc aagcggcgtt
    26641 aacaacttcc cctttaaagt gaacggtccc accattaacc gtcgtggcag cggccagagc
    26701 cgctgtagaa ctgagggaca gagccgacag aacaacgatt gccagagttt taattttcat
    26761 gctgctttcc tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc tgtacacttt
    26821 gttttgtcaa cgagtttgac tgccaacact gcacagtttt cccccaaaag atgaaacatt
    26881 tggggccatt ttgactcata gaggaaagca tcgcgcacaa actttttcag tttatttgtt
    26941 ggcttaatat tctattgtta tctttattta tagattttta tattgcatga ggtggttttt
    27001 ggagagaaga atgaggaaga tgcgtcg
//
LOCUS       DAZFFG010000066        26967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFFG010000066 DAZFFG010000000
VERSION     DAZFFG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.066"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            255..2906
                     /gene="ppc"
                     /locus_tag="ACHJ7J_002990"
     CDS             255..2906
                     /gene="ppc"
                     /locus_tag="ACHJ7J_002990"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO5001192.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            3089..4822
                     /gene="eptC"
                     /locus_tag="ACHJ7J_002991"
     CDS             3089..4822
                     /gene="eptC"
                     /locus_tag="ACHJ7J_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5001193.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHIPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            5037..5888
                     /gene="yijO"
                     /locus_tag="ACHJ7J_002992"
     CDS             5037..5888
                     /gene="yijO"
                     /locus_tag="ACHJ7J_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5001194.1"
                     /translation="MYHDVSYLLSRLINGPLYLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(5875..6216)
                     /gene="frwD"
                     /locus_tag="ACHJ7J_002993"
     CDS             complement(5875..6216)
                     /gene="frwD"
                     /locus_tag="ACHJ7J_002993"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5001195.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(6218..7096)
                     /gene="pflC"
                     /locus_tag="ACHJ7J_002994"
     CDS             complement(6218..7096)
                     /gene="pflC"
                     /locus_tag="ACHJ7J_002994"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO5001196.1"
                     /translation="MTSSVGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(7062..9359)
                     /gene="pflD"
                     /locus_tag="ACHJ7J_002995"
     CDS             complement(7062..9359)
                     /gene="pflD"
                     /locus_tag="ACHJ7J_002995"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5001197.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(9410..9730)
                     /gene="frwB"
                     /locus_tag="ACHJ7J_002996"
     CDS             complement(9410..9730)
                     /gene="frwB"
                     /locus_tag="ACHJ7J_002996"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5001198.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(9745..10824)
                     /gene="frwC"
                     /locus_tag="ACHJ7J_002997"
     CDS             complement(9745..10824)
                     /gene="frwC"
                     /locus_tag="ACHJ7J_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO5001199.1"
                     /translation="MNELMQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGASTDEKEDDLDLDFEIN"
     gene            11133..13634
                     /gene="ptsP"
                     /locus_tag="ACHJ7J_002998"
     CDS             11133..13634
                     /gene="ptsP"
                     /locus_tag="ACHJ7J_002998"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5001200.1"
                     /translation="MALIVEFICELPNGVHARPANHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13646..14308
                     /gene="fsa"
                     /locus_tag="ACHJ7J_002999"
     CDS             13646..14308
                     /gene="fsa"
                     /locus_tag="ACHJ7J_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5001201.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            14319..15422
                     /gene="gldA"
                     /locus_tag="ACHJ7J_003000"
     CDS             14319..15422
                     /gene="gldA"
                     /locus_tag="ACHJ7J_003000"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO5001202.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            15696..16313
                     /gene="yijF"
                     /locus_tag="ACHJ7J_003001"
     CDS             15696..16313
                     /gene="yijF"
                     /locus_tag="ACHJ7J_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO5001203.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQGRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(16340..17245)
                     /gene="yijE"
                     /locus_tag="ACHJ7J_003002"
     CDS             complement(16340..17245)
                     /gene="yijE"
                     /locus_tag="ACHJ7J_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO5001204.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLLRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(17339..19519)
                     /gene="katG"
                     /locus_tag="ACHJ7J_003003"
     CDS             complement(17339..19519)
                     /gene="katG"
                     /locus_tag="ACHJ7J_003003"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO5001205.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(19848..20738)
                     /gene="metF"
                     /locus_tag="ACHJ7J_003004"
     CDS             complement(19848..20738)
                     /gene="metF"
                     /locus_tag="ACHJ7J_003004"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO5001206.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            20814..21059
                     /locus_tag="ACHJ7J_003005"
     CDS             20814..21059
                     /locus_tag="ACHJ7J_003005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001207.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     LTNHVLIASCRIRRKRFIRPNPMIIQYISLETIDPKT"
     gene            complement(21087..23519)
                     /gene="metL"
                     /locus_tag="ACHJ7J_003006"
     CDS             complement(21087..23519)
                     /gene="metL"
                     /locus_tag="ACHJ7J_003006"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO5001208.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLINSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(23522..24682)
                     /gene="metB"
                     /locus_tag="ACHJ7J_003007"
     CDS             complement(23522..24682)
                     /gene="metB"
                     /locus_tag="ACHJ7J_003007"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO5001209.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            24959..25276
                     /gene="metJ"
                     /locus_tag="ACHJ7J_003008"
     CDS             24959..25276
                     /gene="metJ"
                     /locus_tag="ACHJ7J_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO5001210.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            25336..25944
                     /gene="yiiX"
                     /locus_tag="ACHJ7J_003009"
     CDS             25336..25944
                     /gene="yiiX"
                     /locus_tag="ACHJ7J_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO5001211.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LVQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            26089..26764
                     /locus_tag="ACHJ7J_003010"
                     /pseudo
     CDS             26089..26764
                     /locus_tag="ACHJ7J_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(26851..>26967)
                     /locus_tag="ACHJ7J_003011"
     CDS             complement(26851..>26967)
                     /locus_tag="ACHJ7J_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO5001212.1"
                     /translation="ESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
BASE COUNT         6562 a         7488 c         6851 g         6066 t
ORIGIN      
        1 acctacacct ttggtgttac ttgaggcgat tttttaacat tcccataagt tacgcttatt
       61 taaagcgtcg tgaatttaat gacgtaaatt cctgctattt attcgtttgc tgaagcgatt
      121 tcgcagcatt tgacgtcacc gcttttacgt ggctttataa aagacgacga aaagcaaagc
      181 ccgagcatat tcgcgccaat gcgacgtgaa ggatacaggg ctatcaaacg ataagatggg
      241 gtgtctgggg taatatgaac gaacaatatt ccgcattgcg tagtaatgtc agtatgctcg
      301 gcaaagtgct gggagaaacc atcaaggatg cgttgggaga acacattctt gaacgcgtag
      361 aaactatccg taagttgtcg aaatcttcac gcgctggcaa tgatgctaac cgccaggagt
      421 tgctcaccac cttacaaaat ttgtcgaacg acgagctgct gcccgttgcg cgtgcgttta
      481 gtcagttcct gaacctggcc aacaccgccg agcaatacca cagcatttcg ccgaaaggcg
      541 aagctgccag taacccggaa gtgatcgccc gcaccctgcg taaactgaaa aaccagccgg
      601 aactgagcga agacaccatc aaaaaagcag tggaatcgct gtcgctggaa ctggtcctca
      661 cggctcaccc aaccgaaatt acccgtcgta cactgatcca caaaatggtg gaagtgaacg
      721 cctgtttaaa acagctcgat aacaaagata tcgctgacta cgaacacaac cagctgatgc
      781 gccgcctgcg ccagttgatc gcccagtcat ggcataccga tgaaatccgt aagctgcgtc
      841 caagcccggt agatgaagcc aaatggggct ttgccgtagt agaaaacagc ctgtggcaag
      901 gcgtaccgaa ttacctgcgc gaactgaacg aacaactgga agagaacctc ggctacaaac
      961 tgcccgtcga atttgttccg gtacgtttta cctcgtggat gggcggtgac cgcgacggca
     1021 acccgaacgt cactgccgat atcacccgcc acgtcctgct actcagccgc tggaaagcca
     1081 ccgatttgtt cctgaaagat attcaggtgc tggtttctga actgtcgatg gttgaagcga
     1141 cccctgaact gctggcgctg gttggcgaag aaggtgccgc agaaccgtat cgctatctga
     1201 tgaaaaacct gcgttctcgc ctgatggcga cacaggcatg gctggaagcg cgcctgaaag
     1261 gcgaagaact gccaaaacca gaaggcctgc tgacacaaaa cgaagaactg tgggaaccgc
     1321 tctacgcttg ctaccagtca cttcaggcgt gtggcatggg tattatcgcc aacggcgatc
     1381 tgctcgacac cctgcgccgc gtgaaatgtt tcggcgtacc gctggtccgt attgatatcc
     1441 gtcaggagag cacgcgtcat accgaagcgc tgggcgagct gacccgctac ctcggtatcg
     1501 gcgactacga aagctggtca gaggccgaca aacaggcgtt cctgatccgc gaactgaact
     1561 ccaaacgtcc gcttctgccg cgcaactggc aaccaagcgc cgaaacgcgc gaagtgctcg
     1621 atacctgcca ggtgattgcc gaagcaccgc aaggctccat tgccgcctac gtgatctcga
     1681 tggcgaaaac gccgtccgac gtgctggctg tccacctgct gctgaaagaa gcgggtatcg
     1741 ggtttgcaat gccggttgct ccgctgtttg aaaccctcga tgacctgaac aacgccaacg
     1801 atgtcatgac ccagctgctg aatatcgact ggtatcgcgg cctgattcag ggcaaacaga
     1861 tggtgatgat tggctattcc gactcagcaa aagatgcggg cgtgatggca gcttcctggg
     1921 cgcaatatca ggcacaggat gcattaatca aaacctgcga aaaagcgggt attgagctga
     1981 cgttgttcca cggtcgcggc ggttccattg gtcgcggcgg cgcacctgct catgcggcgc
     2041 tgctgtcaca accgccagga agcctgaaag gtggcctgcg cgtgactgaa cagggcgaga
     2101 tgatccgctt taaatatggt ctgccagaaa tcaccgtcag cagcctgtcg ctttacaccg
     2161 gggcgattct ggaagccaac ctgctgccac cacctgagcc gaaagagagc tggcgtcgca
     2221 ttatggatga actatcagtc atctcctgcg atctctaccg tggctacgta cgtgaaaaca
     2281 aagattttgt gccttacttc cgctccgcta caccggaaca agaactgggc aaactgccgt
     2341 tgggttcacg tccggcgaaa cgtcgcccga ccggcggcgt cgagtcgctg cgcgccattc
     2401 cgtggatttt tgcctggacg caaaaccgtc tgatgctccc cgcctggctg ggtgcaggta
     2461 cagcgctgca aaaagtggtc gaagatggca aacagagcga gctggaggct atgtgccgcg
     2521 attggccatt cttctcgacg cgtctcggca tgctggaaat ggtattcgcc aaagcagatc
     2581 tgtggctggc ggaatactat gaccaacgcc tggtagacaa agcactgtgg ccgttaggta
     2641 aagagttacg caatctgcaa gaagaagaca tcaaagtggt gctggcaatt gccaacgatt
     2701 cccatctgat ggccgatctg ccgtggattg cagagtctat tcagctacgg aatatttaca
     2761 ccgacccgct gaacgtattg caagccgagt tgctgcaccg ctcccgccag gcagaaaaag
     2821 aaggccagga accggatcct cgcgtcgaac aagcgttaat ggtcactatt gccgggattg
     2881 cggcaggtat gcgtaatacc ggctaatctt cctctcctgc aaaccctcgt gcttttgcgc
     2941 gagggttttc tgaaatactt ctgttctaac accctcgttt tcaatatatt tctgtctgca
     3001 ttttattcaa attctggata tcccttcaga tatccttaag gaattgccgg tacattcggc
     3061 gatatttttt tcaagacagg ttcttactat gcattccaca gaagtccagg ctaaacctct
     3121 ttttagctgg aaagccctgg gttgggcact gctctacttt tggtttttct ctactctgct
     3181 acaggccatt atttacatca gtggttatag tggcaccaac ggcattcgcg actcgctgtt
     3241 attcagctcg ctgtggttga tcccggtatt cctctttccg aagcggatca aaattattgc
     3301 cgcagtgatc ggcgtggtgc tatgggcggc ctctctggcg gcgctgtgct actacgtcat
     3361 ctacggtcag gagttctcgc agagcgttct gtttgtgatg ttcgaaacca acaccaacga
     3421 agccagcgag tatttaagcc agtatttcag cctgaaaatt gtgcttatcg cgctggccta
     3481 tacggcggtg gcagttctgc tgtggacacg cctgcgcccg gtctatattc caaagccgtg
     3541 gcgttatgtt gtctcttttg ccctgcttta tggcttgatt ctgcatccga tcgccatgaa
     3601 tacgtttatc aaaaacaagc cgtttgagaa aacgttggat aacctggcct cgcgtatgga
     3661 gcctgccgca ccgtggcaat tcctgaccgg ctattatcag tatcgtcagc aactaaactc
     3721 gctaacaaag ctactgaatg aaaataatgc cttgccgccg ctggctaatt tcaaagatga
     3781 atcgggtaac gaaccgcgca ccttagtgct ggtgattggc gagtcaaccc agcgtggacg
     3841 catgagtctg tacggttatc cgcgtgaaac cacgccggag ctggatgcgc tgcataaaac
     3901 cgatccgaat ctaaccgtgt ttaataacgt agttacgtct cgtccgtaca ccattgaaat
     3961 cctgcaacag gcgctgacct ttgccaatga aaagaacccg gatctgtatc tgacgcagcc
     4021 gtcgctgatg aacatgatga aacaggcggg ttataaaacc ttctggatca ccaaccagca
     4081 gacgatgacc gcccgcaata ccatgctgac ggtattttcg cgccagaccg acaagcagta
     4141 ctacatgaac cagcaacgta cacagagtgc gcgtgaatac gacaccaacg tgctgaagcc
     4201 gttccaggaa gtgctgaagg accctgcgcc gaagaaactg atcatcgttc atctgctggg
     4261 tacgcatatc aaatacaaat accgctaccc ggaaaatcag ggcaagtttg atggcaatac
     4321 cgatcatatt ccgccgggat taaacgcgga agagctggag tcatataacg attatgacaa
     4381 cgctaacctg tataacgatc atgtggttgc cagcctgatt aaagacttta aagcagcaga
     4441 cccgaacggt ttcctggttt acttctctga ccacggtgaa gaggtttacg acacgccgcc
     4501 tcataaaacc caggggcgta atgaggacaa cccgacgcgt catatgtaca ccattccgtt
     4561 cctgctgtgg acgtcggaaa aatggcaagc gactcatccg cgtgatttct cacaggatgt
     4621 cgatcgtaaa tacagcctgg cggaactgat ccacacctgg tcagatttgg cgggcttatc
     4681 ttacgacggt tacgatccaa cccgttcagt ggtgaatccg cagttcaaag aaactacccg
     4741 ctggattggt aacccgtaca agaaaaacgc gctgatcgat tacgacacac tgccgtatgg
     4801 cgatcaggtg ggtaatcagt aattatcagc caggcgctat ttgccggatg cgacgcttga
     4861 cgcgtcttat ccggcctaca gatcgcggcg gtagactgga taaggcgttt acgccacatc
     4921 cggcaataca actgccgcaa tgtttacatc gaactcccct caggccacac ttttcaggtg
     4981 gcctttttta ataaggccag ttactgtttt cccgtctaca tacctaaggg aataacatgt
     5041 atcacgacgt cagctatctg ctctcccgcc tgatcaacgg cccgctgtac ctgcgccaga
     5101 tttactttgc cagcagtaac ggcccggtgc ccgatcttgc gtatcaggtc gattttccac
     5161 ggctggaaat tgtgctggaa ggtgagtttg ttgataccgg cgctggagca acgttagttc
     5221 ccggcgatgt gctgtacgtt cctgctggtg gctggaattt tccacaatgg caagcccccg
     5281 ccactacctt tagcgtgctg tttggcaaac agcaactcgg cttcagcgtc gtgcaatggg
     5341 atggcaaaca atatcaaaat ctggcgaagc aacacgtcgc ccgacgcggc ccacgcatag
     5401 gttcttttct gctacaaacg ctcaatgaaa tgcaaatgca gccccaggag cagcaaacgg
     5461 caaggcttat cgtcgccagc ctgcttagcc actgccgcga tttgcttggt agccaaatcc
     5521 agaccgcctc acgcagccag gcattattcg aagctattcg cgattatatc gacgaacgct
     5581 atgcctccgc gcttacccgc gaatctgtcg cacaggcatt ctatatttcg ccaaattacc
     5641 tctcgcacct gtttcaaaaa acgggggcca ttggttttaa cgaatacctg aatcacacgc
     5701 gactggagca cgctaagacg ttattgaaag gctatgacct gaaggtaaaa gaagtggcgc
     5761 acgcctgcgg ctttgttgac agcaactact tttgccgtct gtttcgtaaa aataccgaac
     5821 gctcgccgtc ggaatatcgt cggcagtacc acagccaact gacagaaaaa ccgactactc
     5881 cagaataaga tgggtttgct gtggcgcaga aagcactttg cgcactgcgc tcatcacccg
     5941 ctgcggctca cgcaggaacg cgtagatgct gcattgcacg taacgacaat gttcaaaacg
     6001 ctcggcaccg gcaagctcga tatccgtaat caacagagca acatccgccc gacggatatc
     6061 ctcgtccgct aaacgattct ccgttcccag cgcgccctga gtttcaatgc tgactcccca
     6121 cttctctaac aggcacaact tttccagccg ttccgccgcc atatatgtat gcgccacgcc
     6181 gcttacgcag gcggttactg ccaccaggta tgccatttta acctcccacg gtaacctgaa
     6241 atccggcccg ttctgccatt tcgcgcatcg ttgtcacatc ggctgacgac ggcgcaggca
     6301 cctctttcat cgaccatgtt ttccccagca ggcggtattt cggttcgccg tactgatgaa
     6361 acggtaacag atggatctgc ctgattttca gcgggatcag tacatccagc gcctgctgca
     6421 tattctcccg gctgagcgtg aaaccaggga tcagcggtaa acgcgggatc acgttgacgc
     6481 cctcactcac cagcaaacgc agattctcca gcacgcgtgg caggttcatc ttcaccacat
     6541 cccgcgcctg agtcgcgtcc ataattttta aatcgaacaa cacttcatcg cacaatttcg
     6601 ccagcggtaa cagcttgctg gctggcgcgt ctccggcagt ttcaatggcg catgacacgc
     6661 cccacaaccg cagccgctgt aaaaaacggg tagcaaactc cgcctgcatt aacacttcgc
     6721 cgccagaaag cgtgacgccg ccgccggacg tgcgaaagaa aatgtcatct ttcatcactt
     6781 cccgttccag cgcgtcaagg ctgatatcgc gaccaatccg ttcaaacgcc ccggaggggc
     6841 attcatccgc atcacgcaaa catttcgcac agtgcagaca tttcgcctct ctgcgtaccg
     6901 tctggatttt gccggagatc gactccggat tagcacacca cgggcaaaga tgcggacagc
     6961 ctttaaaaaa gaccaccgta cgaatgccct cgccgtcatt cagtgaataa cgctgaatgt
     7021 tgaaaatgcg cgccacatca ttgcggcgcg tttccacaac gttacagctg atgcgctgtc
     7081 cgacggatga tgtcatcctg gatctccttc gacagttcga caaagaaggc gctgtatccg
     7141 gcaacgcgca ccaccagccc ggcataatct tgtgggcgct gttgcgcttc ccgcaacgtg
     7201 tcggcgttca ccacgttaaa ctgaatatgc tgcaacttaa gctgggtaaa cgcccgtaag
     7261 aagtcggcca gtttgcgtaa tcccgcttca ccttccaggg tcgccggagt gaatttcacg
     7321 ttcagcaacg taccgttaga cagcagcgta ttatcgagct tactgactga cttcagtacc
     7381 gctgttggcc cttgtgcgtc ctggcccagc atgggtgaca agccgccgtc tgccagttgt
     7441 tccccggcaa aacgaccgtc tggcgtcgca ccaaccaccg atcccaacgg gacgtgagca
     7501 gaaacggtat acgatcccgg cgtgaagtaa ccgccgcgcg ggttctggta tttttccact
     7561 tctttgcagt agtggcgcaa cagttcggcg ctaatgttat ccacctcgtc gatatcgtta
     7621 ccgtatttct caaagcggtt aattaagcga gcgcggactt tttcgccttc tggcgttgca
     7681 aagttggctt ttaataccga cagcaattcg tcaaaactta aacgctgttg atcaaaaacc
     7741 atccctttca acgcatggag agaatcgctc aggttggcga taccaattcc ctgtacgccg
     7801 gagaagttat aacgcgcgcc gccatcggta atatcgcgac ctttttccag acaatcgctg
     7861 ataaaagacg agagcagcgg tacaggtgcc cagtcgcgat ggccgatatc acaaatattg
     7921 ctgccctcaa ccatcagggt gatgtagtgg ctgatcttgg cgcggatctg ctccagtaaa
     7981 ccttcataag tcagcgcagc attgccttca ttttcatgca ggcagatttc catcactttc
     8041 agcagattaa acatcgcgat gtcatgcaag ccgtaggttc tgccgggaat agataattcc
     8101 acacagccca ctacggaata gtcgcgcgca tcttccagcg aaacgccacg gttgaggaac
     8161 gctggcacca ccacttcatc gttaaagatt tgcggaatac cggtgccgag gcgaatggtt
     8221 tctgcggttt tcatcaggaa cggcgtgtcg atcaaggcgt tagtgcgcac ccctaggttc
     8281 ggttgcggta attgtacgct ttgataggca tcgaggcaga ggaacgaaag cacgttcacc
     8341 gcgctacgtc cgttctcggt taacccgccg agcagtgcgg tatagccagt cgggaaacct
     8401 gcgaaataac gcgcgctgct ggtggagcgc aacagcacga tgtcgttgca tttcacccat
     8461 aaagattcga gcagttcttt caggaacgcc ggatcttcgc cctgggttaa agatgcctga
     8521 tagaacggca acatatactg gtcgaagcgc cctaaagaca gcgaactggc gttggattcg
     8581 tattgcagaa tgatgttcat gtaccagaat aactggcacg cctgccagaa cgtctgcggc
     8641 ttatgttcgg cgttatggcg ggagatctcc gcaatagtca gcagttcttc gcgacgctgg
     8701 gcatcagtgc agcttgccgc catcgtttcc gccagttcgg cgtaacgcaa aatgtgtttc
     8761 tgcgaggctt cgagcagtaa cagcgctgcc tgataaaagt gattctccgg ctgttgctga
     8821 caatgttgct gcatttgcgc taccagctca cccagcccgt gattcagcag gcgcgggtaa
     8881 tcaataataa tgtgcccctg gcctttatcc gtctggttga tgctgaaaat ctgcgtgctg
     8941 gtcgcggctt ttacttcatc cgtcatctgc ccgttgatga aatctttcat cgaacgtttt
     9001 tcccagtacg ggaacaactc ttcacggtag atacgtttat cttcttcgct gatagcaaag
     9061 cggtcctgcg gacgcgtcgg gaactggtcc aactctttca gcaaccagta agggtccatt
     9121 tccggcgaca taatcccggc gcgcggtttc accgtgcggt taccggcaat cagttcttca
     9181 tcacgaatcg aaatttcaac atgttcaagg atatacgctg ttgctttcgc ccggcgcaat
     9241 atcaccggtt cgccttcggt ttgccgatgg ctggcggtat aaagcagcgc acgctccagc
     9301 gagatttcac gggtattggc aaacagtgca gttttgaggc gagagatacg attcgtcatg
     9361 aggaactcct gtgggaaagc gaacccgccc ttgcgagcgg gagaagaact taagcggttt
     9421 gcgccaggtg agcttcaatt ttgctcataa tggcgtcagc gcgttttacc gcgtcgctga
     9481 tgttgacgcg cacaatggtc ttgccagcaa aacgctcttc aaacttgatg ccgatatctt
     9541 tggtcagaat caccatatcg gcgctcgcca catcttccgc agtcagttcg ttttccagac
     9601 caatcgagcc ctgggtttct actttcactt cccagccttt cgctttcgcg gcactttcca
     9661 gcgcctctgc cgccatatag gtatgggcaa cacctgaagg acatgcggtt actgcaataa
     9721 ttttcgtcat ggctcgttcc tcaattaatt aatttcaaaa tccaaatcca ggtcgtcttc
     9781 tttttcatca gtcgaagccc catttttacg cgccagactt ttcagcacgt tcacacaaac
     9841 agccgtcaca accgccccga cagccactgc tgcgatataa cccaacttac cttcaaccac
     9901 cggcagcaca atcagaccac cccagcctgc atagcactgc gcaccgacca gcgccgcagt
     9961 tactgcacca caaactgaac cgaccatgat ggaaggaata acacgcagcg gatcggcagc
    10021 ggcgaaagga atcgcccctt cagtaacccc aacgcacccc atcaccagcg ccgctttacc
    10081 ggtttcgcgc tcttctgcgg agaaattttt acgaccaatc agcgtcgcca gccccatccc
    10141 cagcggcggg atacaaatac ccacggcagc aatggccacc acggtataaa caccctgagc
    10201 aacgcaaatc agcatgaagg cataggccac tttgttaacc ggaccgccca tatcgaacgc
    10261 cagcatcaga cccatgatca ccgccagcat aacaatgctg ccctgctgca tcccctgaag
    10321 ccactgagtc aggctgttgg tcaacgcccc tacaggctcg cccaagcccc acatcatgat
    10381 gcctgcggta atcagtgtgc cgatgatagg aatgatgaag ataggcatca ccgagcgcag
    10441 aactttatgc accggaattt tcttcaggta atgcaccact atgccgccga taatcccggc
    10501 gatcagcgca ccaaagaagc ccgcaccaaa gctgttacca acccaggcac cgatagcgca
    10561 cggagccagc gcagaacgct ctgcaatgga gtaaccaatg taagcggcga ggaaaggcac
    10621 catcagcgtc aaacctgcaa cgccgatatc aaacagtttt ttcagattcg gatcggctac
    10681 ggcatccggc actgcgcctt tgccatacaa catgacggaa accgccagca aaataccgcc
    10741 cgataccacg aagggaatca tgtgtgaaac gcccgtcatt aagtgctgac gggtattttt
    10801 caggatctgc atcaactcat tcataagctg ctcccgggct tgcgaaagcc catgtccata
    10861 tttttgatgg aggaaacaat aggcaatcgc aggcggagca aacagtggat aaaaaggcca
    10921 tttactggac ttttgtaata tcagtacaaa aatgcgatcc gcctcataac ttgcgataat
    10981 ccggaagcat gccgcaaaac ggcgtcttgt gagacgcgtc gcaaattgac ggttacatta
    11041 catttgtcca gcttttggca gatttgtcac atggcaggcc gttgccgtgc gctcttatcc
    11101 tgtaaacaga aaaccgtacc gggagagagg caatggccct gattgtggaa tttatttgtg
    11161 agctacctaa cggcgtacat gcacgtccgg caaaccacgt tgaaacgctg tgtaatactt
    11221 tttcatcaca aattgagtgg cataacctgc gcactgaccg caagggcaac gccaaaagcg
    11281 cccttgcgct gattggcacc gacacgctgg cgggcgataa ctgccagtta ctgatttccg
    11341 gggccgacga acaggaagcg caccagcgtt taagccaatg gctgcgcgat gaattccccc
    11401 actgcgatgc gccgctggcg gaagttaaat ctgacgaact ggaaccactg ccggtttcac
    11461 tgaccaatct gaatccgcaa attatccgcg cccgcaccgt gtgcagcggt agcgcaggcg
    11521 gcattctgac gccgatctct tctttagatc tcaatgcgct gggtaatctt cccgcagcca
    11581 aagacgttga cgccgagcaa tccgcactgg aaaacggcct gacgctggta ctgaaaaaca
    11641 ttgagtttcg tctgctggat agcgacggtg ccaccagcgc gattctggaa gcgcaccgat
    11701 ccctggctgg cgatacttcc ctgcacgaac atttactggc aggcgtcagc gccggattaa
    11761 gctgcgccga agcaattgtt gccagcgcga atcacttttg cgaagagttc gcccgttcca
    11821 gcagcagcta cctgcaagaa cgtgccctgg acgtacgcga cgtctgcttc cagttactcc
    11881 agcaaatcta cggtgagcaa cgcttcccgg caccgggcaa actgacgcag cccgccattt
    11941 gtatggctga tgaactgacc cccagccagt tcctcgaact ggataaaaat cacctcaaag
    12001 gtctgttact gaaaagtggc ggcaccacct cacataccgt aattcttgcc cgttcgttca
    12061 acattccgac actggttggt gtagatattg atgcccttac tccgtggcag catcaaacga
    12121 tttatatcga cggcaacgcc ggggcgattg tggttgagcc aggggaagcc gtagctcgtt
    12181 attatcagca agaagcccgc gtacaggacg cccttcgtga gcaacagcgt gtctggctga
    12241 cccaacaagc ccgtaccgct gacggtatcc gcattgaaat tgccgctaac atcgctcact
    12301 ccgtggaagc gcaggccgca ttcggcaatg gtgcggaagg cgtcggtttg ttccgcactg
    12361 aaatgctcta tatggatcgc accagcgcac cgggcgaaag cgagttgtac aacatttttt
    12421 gtcaagcgct ggagtccgcc aacggacgca gcattattgt gcgcactatg gatattggcg
    12481 gtgacaaacc cgttgattat ctgaacattc cggcagaagc taacccgttc ctcggttatc
    12541 gcgcggtgcg tatttatgaa gaatacgcct cgctgttcac cacacaacta cggtcgatcc
    12601 tccgtgcctc tgctcacggc agcctgaaaa tcatgatccc gatgatctcc tcaatggaag
    12661 agatcttatg ggtgaaagaa aaactggcgg aagccaaaca gcaactgcgt aacgaacaca
    12721 ttccgtttga tgagaagatc cagctcggca tcatgctgga agtgccgtcg gtgatgttca
    12781 tcatcgatca atgctgcgaa gagattgatt tctttagtat tggtagtaat gacctgacgc
    12841 aatatctgct ggcagtggat cgcgataacg ctaaggttac tcgtcactac aacagcctga
    12901 atccggcatt cttgcgggcg ctcgattacg ccgtgcaagc ggtgcatcgc cagggcaaat
    12961 ggattggtct gtgcggtgag ctgggagcga aaggttccgt gctgccgttg ctggtcggct
    13021 tagggctgga tgaactcagc atgagcgcac catcaattcc ggcggcgaaa gcgcggatgg
    13081 cgcaacttga tagccgtgag tgccgcaagt tgctcaacca ggcaatggcc tgccgtactt
    13141 cgctggaagt ggaacacctg ctggcgcaat tccgcatgac ccaacaagac gcaccgctgg
    13201 tcaccgccga gtgcatcaca ctggaaagcg actggcgcag caaagaagaa gtactcaaag
    13261 gcatgaccga taacctgctg ctggcgggcc gctgccgcta tccgcgtaaa ctggaagccg
    13321 acttgtgggc gcgcgaggcc gttttctcta ccggtctggg ctttagtttt gccattcccc
    13381 acagcaaatc agaacacatt gagcaatcca ccatcagcgt agcgcgtctg caagcgccgg
    13441 tgcgctgggg cgatgatgaa gcgcaattca tcattatgtt aaccctgaac aaacacgctg
    13501 cgggcgatca gcatatgcgc attttctcgc gcctcgctcg ccgcatcatg cacgaagaat
    13561 tccgtaacgc gctggttaac gccgcctctg ccgacgctat cgccagcctg ctgcaacatg
    13621 aactggaact gtaaaaggaa acatcatgga actgtatctg gacaccgcta acgtcgcaga
    13681 agtcgaacgt ctggcacgca tattcccgat tgccggggtg acaactaacc cgagcattat
    13741 cgctgccagc aaggagtcca tatgggaagt gctgccgcgt ctgcaaaaag cgattggtga
    13801 tgagggcatt ctgtttgctc agaccatgag ccgcgacgcg caggggatgg tggaagaagc
    13861 gaagcgtctg cgcgacgcca ttccgggcat tgtggtgaaa atcccggtga cttccgaagg
    13921 tctggccgcc attaagatgc tgaaaaaaga gggtattact acgcttggca ccgcagtgta
    13981 cagtgctgca caagggttac tggccgcgct ggcaggggca aaatacgttg ctccgtatgt
    14041 taaccgcgta gatgcccagg gcggggacgg cattcgtacg gttcaggagc tgcaagcgct
    14101 gttagaaatg cacgcaccag aaagcatggt gctggcggcc agctttaaaa cgccgcgtca
    14161 ggcgctggac tgtttactgg caggatgtga gtccatcacc ctgcccttag atgtagcgca
    14221 acaaatgctc aacacccctg cggtagagtc agctatagag aagttcgaac acgactggaa
    14281 tgccgcattt ggcactactc atctctaaag gagcaattat ggaccgcatt attcaatcac
    14341 cgggtaaata catccagggc gctgatgtga ttaatcgtct gggcgaatac ctgaagccgc
    14401 tggcagaacg ctggttagtg gtgggtgaca aatttgtttt aggttttgct caatccactg
    14461 tcgagaaaag ctttaaagat gctggactgg tagtagaaat tgcgccgttt ggcggtgaat
    14521 gttcgcaaaa tgagatcgac cgtctgcgtg gcatcgcgga gactgcgcag tgtggcgcaa
    14581 ttctcggtat cggtggcgga aaaactctcg atactgccaa agcactggca catttcatgg
    14641 gtgttccggt agcgatcgca ccgactatcg cctctaccga tgcaccgtgc agcgcattgt
    14701 ctgttatcta taccgatgag ggtgagtttg accgctatct gctgttgcca aataacccga
    14761 atatggtgat tgtcgatact aaaatcgtcg ctggcgcacc tgcacgtctg ttagcggcgg
    14821 gtatcggcga tgcgctggca acctggtttg aagcgcgtgc ctgctctcgt agcggcgcga
    14881 ccaccatggc gggcggcaag tgcacccagg ctgcgctggc actggctgaa ctgtgctaca
    14941 acactctgct ggaagaaggc gaaaaagcga tgcttgccgc cgaacagcat gtagtgactc
    15001 cggcgctgga gcgcgtgatt gaagcgaaca cctatttaag cggtgtaggc tttgaaagtg
    15061 gtggtctggc tgcggcgcac gcagtgcata acggcctgac cgctatcccg gacgcgcatc
    15121 actattatca cggtgaaaaa gtggcattcg gtacgctgac gcagctggtt ctggaaaatg
    15181 cgccggtgga ggaaatcgaa accgtagctg cccttagcca tgcggtaggt ttgccaataa
    15241 ctctcgctca actggatatt aaagaagatg tcccggcgaa aatgcgaatt gtggcagaag
    15301 cggcatgtgc agaaggtgaa accatccaca acatgcctgg cggcgcgacg ccagatcagg
    15361 tttacgccgc tctgctggta gccgaccagt acggtcagcg tttcctgcaa gagtgggaat
    15421 aacctactcc aaactcccgg cttgtccggg agtttgaacg caaaattgcc tgatgcgcta
    15481 cgcttatcag gcctacgcaa tctctgcaat atatttaatt tgcgtgcttt ttaggccaaa
    15541 taaggcgttc acgccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc
    15601 aggaattttt tatccgcttc tggacatttt ctctacagaa caatcgttag cccggaaggt
    15661 cgaatctgtg actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca
    15721 gcctgctaac ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg
    15781 tacaaatcca ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca
    15841 gcacgctatt ttacgatccg gcgtatgtgc agcttaccta tcctggcggt gatgtcccgc
    15901 aaggacgcgg ggtatgttcc gatgtggtga tccgtgcatt gcgcagccag aaggtcgatt
    15961 tgcagaaact ggtgcacgaa gatatggcga agaattttgc cgagtacccg caaaaatggc
    16021 agttaaagcg cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt
    16081 ttacccgcca cgataaaaca cgccccatca gcaagaaccc cagtgactat caagcgggcg
    16141 atattgtctc ctggcgatta gataacgggc tggcacatat tggggtggtt tcagatggct
    16201 tcgcccgcga cggtacgccg ctggtgatac ataacattgg cgcaggtgcg caggaagaag
    16261 atgtgctgtt tagctggcgc atggtggggc attatcgtta ttttgttaaa taagcgatcg
    16321 ccccacatga agaaaaaatt cagatccttt ttacactaac ggcttctttt ttcttacggc
    16381 tcaccagcgc cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat
    16441 tctcaccgag cagccaccag gaaaagagta cgccgcaaac gggaacggcc agtgtgctta
    16501 agctggcaat actggcaggc aagtttttca gcacaaacag ccataagctc cacgccagcg
    16561 ccgtcgccag aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt
    16621 cacgttgcgg caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc
    16681 aggatgttaa cgacaataaa tccacgcgcg gatgacgggc atacagacgt ttagcaacaa
    16741 tcgcgctcgc cccccaacta acaccggaga ggattgccag catggcactt ttcatagaag
    16801 aaaaatcgag ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg
    16861 cgaaatattg cccacgtagc aggcgttcac cgagaaacac cgcggcgaaa atcaccaccc
    16921 agaacggcat ggtatagctc aggatcgcca ccttccccgc tccgccgctg accagcgccc
    16981 actgcgccag gccaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa
    17041 acggtgtcgg gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc
    17101 cgaaaatgca gcgtaaggcg gtaaagtcga aggcaccgat gtagctggtg acttgcttca
    17161 tgaaaatcca gctataactc cagataagtg tgagcacaac caggccactg attgccagtg
    17221 ggttgctctt tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta
    17281 tacgccggta aagaaacttc agcgactgcc tgaaaggcag tcgctgaaga aggtcagatt
    17341 acagcaggtc gaaacggtcg aggttcatca ctttcaccca tgccgccacg aagtctttaa
    17401 caaacttctc gtgagcatcg ctgctggcgt aaacttcagc caccgcacgc aggacagagt
    17461 tagaaccaaa taccagatcc gcacggctgg cagtgtattt cacttcgccg gtttcgcggt
    17521 cacggccttc gaacagctct ttcgattcgt cggtcgcttt ccactcgtaa cgcatatcca
    17581 gcaagttcac gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt
    17641 tgctgccatc gaagttggca cccagtacac gcatcccgcc caccagcgcg gtcatttccg
    17701 gcgcggtcag ggtcagttgc tgggctttat caatcagcag tgactcggtg gtggaaacgt
    17761 ccagacgagc gcggtagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacattt
    17821 caatgtcagt ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc
    17881 tcaaaccagc ggcgcttgcg gctttctcaa cgccaaccac acctgccagc acgatgatat
    17941 ccgccagcga ggctttacca gactctttct gaattttctc cagaactggc agcgcacgaa
    18001 cggctgctgc gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg
    18061 caccaccgcg tttgtcgcca ccacggaagg tagaagcaga agcccaggcc accgatacca
    18121 gctcactaac agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct
    18181 cggtcgggtt gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca
    18241 cttccggccc gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg
    18301 cacgggcaaa ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct
    18361 cgaactcagg atcaaagcgc agcgtcaggt cggtcaccag catcgtcggt ttacgtttct
    18421 tcgacggatc aaacggatcc ggaataattt ccggtgcgtc taccgcttcg aactggattg
    18481 cgccagccgg gctgcgggtc tgtacccact catacttgaa caggttctcg aagaaatagt
    18541 tgctccactg ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg
    18601 cgccaacgcc gctgccgtaa gtgctcgccc aacctaaacc ttgttcttca atcggtgcag
    18661 cttctggatc aggacctaca tttgatgtcg gaccagcacc gtgggtttta cccagcgtat
    18721 gaccacccgc aatcagcgcc actgtttctt cgtcgttcat acccatgttg ccgaaggtcg
    18781 cgcggatagc tgctgccgca gaaagcggtt cgccgctgtg atcagggcct tccgggttaa
    18841 cgtaaatcag gcccatctcg gttgcgccca gcggtgcttt cgccagcgct tccggatgac
    18901 ggtgagtcag ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt
    18961 cttcacgacc ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac
    19021 ccgcgaggat aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc
    19081 acaacaggcg acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa
    19141 aacgttgctg accacgaccc gcgccgccgc gcccatcgat tgaacggtaa gtacccgcac
    19201 cgtgccaggc catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg
    19261 gttgagattc tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc
    19321 tgaattcttt gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt
    19381 ttaacaggtc aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg
    19441 cgctctggtc gtgaccgccc tgatggaacg ggcatttgcc agtggctgtc gtgttatgga
    19501 tatcgtctga cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag
    19561 cgttagagag aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat
    19621 tgttggttaa agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga
    19681 attcggacaa atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga
    19741 gaaaacaagg ctgataacct tattttccag ccctcatttc gaggcagcat tttgtgctct
    19801 gtttaaaatt tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt
    19861 cgaaccccca gcgtatggca aatcgcgtaa ctcatttcag cacggttaag cgtatagaag
    19921 tggaaatctt tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg
    19981 ccaaccagtt tgcgggtttc ggcatcatca tccaggccgt cgaacatttg cgccatccac
    20041 gccggaatac gcacgttggt catatcggca aatttcttcg cctgtttaaa gttagatacc
    20101 ggcaaaattc ccggaataat ttccacatca atgcccgccg atacacagcg gtcacgaaaa
    20161 cgcaggtagc tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcg
    20221 actttgcgtt tcaggttaag caaatccgcc tgagcgcttt ttgcttctgg gtgaacttcc
    20281 ggatacgccg ccacggagat atcgaaatct gccacttctt ttaatagcgt caccaggtca
    20341 gaagcataca tttctggctt accacttccc ggcggcagat cgccacgcag cgccacgata
    20401 tgacgaatac cgttattcca gtagtcgcgt gcaatggtgc gcaactcgtc gggcgtcgca
    20461 tcaatgcaag taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata
    20521 atgctgtgcg tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc
    20581 ggtttcaggc tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta
    20641 cgcggcggga aaaactcgaa cgaaacgtta atctgcccct ggacttctgc caggctctga
    20701 ttcagggcat cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg
    20761 tagttatatg ttgtgttgtg cacatctatc cgtttagacg tccagatgta aagatgaagg
    20821 aaaagccgaa gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga
    20881 tgaaaaaact tcaggatggt aagaaaaaga aaatccggta tgcaaagcaa accggactgc
    20941 aaatattaac taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc
    21001 ggcctaatcc tatgataatt caatatattt cattagaaac catagacccg aaaacatgag
    21061 taccgggcat tattaaattt ctgaaattac aacaactgcg ccagccggtt gatatccgac
    21121 tgaatcgccc cggcggtgac gtcgcgcccc gcgccaggcc cacggatcac cagcgggttg
    21181 tcgcgatacc agcggctttc gatggcaaag acgttatcgc acggcagcag tgatgccagc
    21241 ggatggtctt cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc
    21301 gccacgtagc gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc
    21361 tcgttaagtt catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca
    21421 ggcaccagcg attccacacg tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc
    21481 agaatcacca gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg
    21541 gttaagccct gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat
    21601 tgcaggaaca gccaggagag cgtgccggag aagatcccac tgatcgacaa aatagtatcg
    21661 ccgctgttga tcagatcgcg cacggtatgg ttgatcggca agcccgcacc gacggtggca
    21721 ttgtacagcc agtgacgccc tgttttttca aaggcgtcgt ggatctggcg atatttattg
    21781 ctgtcgcttg ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa
    21841 tcaagatact ggtcagcaag ctgctggctg gcggtaacgt ccagcacgac taaatcatca
    21901 tacggatggg cgcgcatcca caggaacaac gactcttcat cctgctcgac cgcttcatcg
    21961 ttgaagaagg ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg
    22021 ctgtccacca cgcctgccag cacaaattca aagccagtac gcgccgaaag cgtgctctgc
    22081 tcgcgggcga acagttccag ccagcgggaa ccgatattgc ctttgccgaa caataccagg
    22141 ccgatgcgtt tttctgcgcg gaagacggac tgatgcaggc cctgaatcag gctttcggtc
    22201 gggccggtgc gcagtactgc caccaggctg atgccgtcat cggactgcca ggtaaactcg
    22261 accggctggc ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtaaca
    22321 cccgccccga ccatcgccac cagcgccagc ccctgacgca ggcgcagttc gccaggtaat
    22381 cccgcttcgt cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc
    22441 agcaactggc gatcgttatg cacgccaact gccagtggac gcacctgggc acgtttcagg
    22501 atttggtcga tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca
    22561 atcaaacaga catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg
    22621 cgttcaatgc gcgtggagcc ttgatccggc gtgtagctac agcgcagctg caggtcgatt
    22681 tcgctaccgg atactggctg taaagtacga gcgtgaagaa cgggagccgc cagacgcgcc
    22741 agttcgctgg cttcatccag acgcagcaat ggcagcagac aggcatcttt cactttacgc
    22801 ggatcggcac tgtatacccc ggcgacgtcg ctccagatgg ttacgcgaga aacacccgcc
    22861 agcgcaccga tttgtgtcgc ggaatagtcg gaaccgttac gccccagcag caccgtttca
    22921 ccggcgttgt tacggctgat aaacccggtc accaccagac gtttgcccgg atgttgcacc
    22981 agtagctgtt gcagcaacgg gtaagaaagc ccttcatcaa cctgcggctg cgcggcgcgt
    23041 tcagcgcgta aaaactcgcg ggcatcaagc caggccgctg gcagcccttg ttgattaagc
    23101 accgcagaca taaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc
    23161 gcatcgttaa taccactgtc gagcagcgcc gccaggcgct caagatcgct gacaaaagcg
    23221 ctaatgaggc tatcggcttc ttcggcgggt agcaggccgc taatcagatc gcactgatag
    23281 cgacgcagcg tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac
    23341 cagttaatca actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc
    23401 tgagagtact ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta
    23461 ctgccaccaa atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt
    23521 tttacccctt gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat
    23581 cttcaatacc ggtggagata cgcagcaacg tctcggagat cccggcggca gcacgcgctt
    23641 ctggtgccat gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc
    23701 ctaatgattc cgccagcgta aacaacgaca gcccgcccag gaaacgacgc agcgtctgct
    23761 catcgccatc cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa
    23821 tttcatgccc ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg
    23881 tttgcagata tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca
    23941 gcgttcgcaa cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat
    24001 tgtttgccca ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag
    24061 ccaccacgtc agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac
    24121 ccaatgccag cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg
    24181 ccccgacttc ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat
    24241 tacttgggct ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct
    24301 gttcatcgcc ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc aggctgtcga
    24361 acaggcgata gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca
    24421 aaaagacggt cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcaccag
    24481 caccgccttc cagttctgcc agtgcacgct gaaccacatc gcgcgttggg ttgccgcgac
    24541 gcgagtaatc atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat
    24601 ggatgggagg gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga
    24661 tggtggcctg tttacgcgtc atgtgatgaa gttccctggg cttggtcggt gaaatgtcag
    24721 gcaccagagt aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg
    24781 cgtatagatt gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag
    24841 gtgcattaca cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt
    24901 acccacataa attgtgtggt ctggtctcaa tttattgacg aagaggatta agtatctcat
    24961 ggctgaatgg agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca
    25021 agtcaaaaag attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg
    25081 cacgcgtcgt caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc
    25141 gtttctgcat gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg
    25201 cagcgacgaa atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga
    25261 gacgtgggaa tactaaagcg gaaaagagcg gcgcggagtt aaccagccgc ttttttcata
    25321 caaggacgtt taacgatgaa aatcaggcta ctgatcctca gcctgctggt ttctgtgcct
    25381 gcctttgcct ggcagccgca aactggcgac atcatctttc aaatctctcg ctcatcgcaa
    25441 agtaaagcga tccaactggc gacccattcc gattacagcc acaccggtat gctggtgatg
    25501 cgtaacaaaa agccctacgt ttttgaagcg gtcggcccgg tgaaatacac cccgctcaag
    25561 cagtggatcg cccatggtga aaagggcaaa tacgttgttc gccgcgttga aggcggactg
    25621 agtgttgaac aacagcaaaa actggtgcaa acggcgaaac gttatctcgg caaaccgtac
    25681 gatttcagct tctcatggag cgacgatcgc cagtactgtt cggaagtggt gtggaaggtt
    25741 tatcagaatg cgctgggaat gcgcgtgggc gaacagcaga agttgaaaga gtttgatctc
    25801 agcaacccgc tggtccaggc gaagctcaaa gaacgctacg gcaaaaatat tccgcttgag
    25861 gaaacggtag tttcaccaca agccgttttc gacgcgccac aactcaccac ggtcgccaaa
    25921 gaatggccgc tgttttcgtg gtaatggatg ataaaaaaaa taattcccgc cgtagtctgt
    25981 ttgttgcttt gtagttgcac gacctttccg caagctgtcg ctccggtaaa cggtgatgcc
    26041 tccaattagt tgaattaagg atataatact cgcttttaag gttgtttcat ggtcacagtt
    26101 aaccttcatt gtccccgttg ccagtccgtt caggtttacc gtcacgggca gaaccccaaa
    26161 agacatgatc gattccgcgg ccgtgactgt caccgggtat ttcagctcag ttactgttac
    26221 gagccccgta aaccgggagt taaagaccaa atcgctgaaa tggctttcaa cggtgccggg
    26281 gttcgctata ctgcccgtac gctgaaaatc ggcattaata cagtcatccg aactttaaaa
    26341 aactctcgcc acgaagaata acgtcatccc cggtcgctca tgctgatgtg gcactcatct
    26401 gcgaacttag tcagcaatgg agctttgtcg gcagtaaagc gcgacagcac tggctctggt
    26461 acgtgtacaa cacgaaaact ggtggtgttc tggcctacac ttttggcccc cggactgatg
    26521 aaacctgtcg tgaattgctg gcgctgctta cccttttacc atcggcatgt tgaccactga
    26581 cgaatggggt agttacgcta gagagttacc aaaggagaag catctgactg gtaagatttt
    26641 tactcagcac attgagcgta acaatctgac cctgagaacc cgaatcaaac gtctggcccg
    26701 caaaaccatc tgtttctcga gttctgtaga acttcatgaa aaagtcatcg gtgcttttat
    26761 cgaaaatata tgttctacta attggagtca tcacccttct gatgagcttt ccttggatga
    26821 ttaccagact gaatacacag ccaaagattt ttacctcatt ctgctctttt ttctcaaatg
    26881 acatatatta tattttacat catattcata ggcatatgac attcccccgc cccccattat
    26941 gtcattgtgg gttgatgttg ggctttc
//
LOCUS       DAZFFG010000067        26720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFFG010000067 DAZFFG010000000
VERSION     DAZFFG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.067"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            220..729
                     /locus_tag="ACHJ7J_003012"
     CDS             220..729
                     /locus_tag="ACHJ7J_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO5001213.1"
                     /translation="MNLQRFDDSTLIRIFALQELHRLKERGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQNLDDYYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            726..2144
                     /gene="phrB"
                     /locus_tag="ACHJ7J_003013"
     CDS             726..2144
                     /gene="phrB"
                     /locus_tag="ACHJ7J_003013"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO5001214.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVELVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GTGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSSGWMHNRLRMITASFLVKDLLINWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2294..3775)
                     /gene="dtpD"
                     /locus_tag="ACHJ7J_003014"
     CDS             complement(2294..3775)
                     /gene="dtpD"
                     /locus_tag="ACHJ7J_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO5001215.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            4046..4789
                     /gene="ybgI"
                     /locus_tag="ACHJ7J_003015"
     CDS             4046..4789
                     /gene="ybgI"
                     /locus_tag="ACHJ7J_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO5001216.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4812..5468
                     /gene="pxpB"
                     /locus_tag="ACHJ7J_003016"
     CDS             4812..5468
                     /gene="pxpB"
                     /locus_tag="ACHJ7J_003016"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO5001217.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5462..6394
                     /gene="pxpC"
                     /locus_tag="ACHJ7J_003017"
     CDS             5462..6394
                     /gene="pxpC"
                     /locus_tag="ACHJ7J_003017"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO5001218.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6384..7118
                     /gene="pxpA"
                     /locus_tag="ACHJ7J_003018"
     CDS             6384..7118
                     /gene="pxpA"
                     /locus_tag="ACHJ7J_003018"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO5001219.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            7154..7945
                     /gene="nei"
                     /locus_tag="ACHJ7J_003019"
     CDS             7154..7945
                     /gene="nei"
                     /locus_tag="ACHJ7J_003019"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO5001220.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTDEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSLRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7942..8988)
                     /locus_tag="ACHJ7J_003020"
     CDS             complement(7942..8988)
                     /locus_tag="ACHJ7J_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5001221.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9137..10198)
                     /locus_tag="ACHJ7J_003021"
     CDS             complement(9137..10198)
                     /locus_tag="ACHJ7J_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5001222.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10195..10926)
                     /locus_tag="ACHJ7J_003022"
     CDS             complement(10195..10926)
                     /locus_tag="ACHJ7J_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5001223.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKNPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10941..13400)
                     /locus_tag="ACHJ7J_003023"
     CDS             complement(10941..13400)
                     /locus_tag="ACHJ7J_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5001224.1"
                     /translation="MDAVNIYRLSVLSCLAMVTLPALAAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWLGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITPLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTNRLILPCH"
     gene            complement(13450..14016)
                     /locus_tag="ACHJ7J_003024"
     CDS             complement(13450..14016)
                     /locus_tag="ACHJ7J_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5001225.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGNGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVVLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14403..15686)
                     /gene="gltA"
                     /locus_tag="ACHJ7J_003025"
     CDS             complement(14403..15686)
                     /gene="gltA"
                     /locus_tag="ACHJ7J_003025"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO5001226.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(15892..15999)
                     /locus_tag="ACHJ7J_003026"
     CDS             complement(15892..15999)
                     /locus_tag="ACHJ7J_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001227.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16380..16784
                     /gene="sdhC"
                     /locus_tag="ACHJ7J_003027"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             16380..16784
                     /gene="sdhC"
                     /locus_tag="ACHJ7J_003027"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5001228.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16778..17125
                     /gene="sdhD"
                     /locus_tag="ACHJ7J_003028"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             16778..17125
                     /gene="sdhD"
                     /locus_tag="ACHJ7J_003028"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO5001229.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            17125..18891
                     /gene="sdhA"
                     /locus_tag="ACHJ7J_003029"
     CDS             17125..18891
                     /gene="sdhA"
                     /locus_tag="ACHJ7J_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO5001230.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAISANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            18907..19623
                     /gene="sdhB"
                     /locus_tag="ACHJ7J_003030"
     CDS             18907..19623
                     /gene="sdhB"
                     /locus_tag="ACHJ7J_003030"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO5001231.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            19924..22725
                     /gene="sucA"
                     /locus_tag="ACHJ7J_003031"
     CDS             19924..22725
                     /gene="sucA"
                     /locus_tag="ACHJ7J_003031"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5001232.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            22740..23957
                     /gene="odhB"
                     /locus_tag="ACHJ7J_003032"
     CDS             22740..23957
                     /gene="odhB"
                     /locus_tag="ACHJ7J_003032"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO5001233.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            24323..25489
                     /gene="sucC"
                     /locus_tag="ACHJ7J_003033"
     CDS             24323..25489
                     /gene="sucC"
                     /locus_tag="ACHJ7J_003033"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5001234.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            25489..26358
                     /gene="sucD"
                     /locus_tag="ACHJ7J_003034"
     CDS             25489..26358
                     /gene="sucD"
                     /locus_tag="ACHJ7J_003034"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5001235.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         6496 a         6677 c         7270 g         6277 t
ORIGIN      
        1 tggcaatata aagtcagcaa tacaacaaat tataaaatat aattgatggt gaaatatgca
       61 gcggtactac caatcggtca agtattcgct gctttgatga tgatgtaaaa tgattgtttt
      121 tgaactggat ataacagctt ggtagggaag aaagcataac cccacaaaat tcatttacca
      181 tcgtctacac ttactcctgt aaaccgttca ggagcagtaa tgaatctaca acgatttgat
      241 gacagcaccc ttatccgcat ttttgctttg caggagttac accgtcttaa agagcgaggc
      301 ttaacgcgcg gggcgcttct cgattatcac agccgctata aactcgtctt tctggcgcat
      361 tcccagccgg aataccgcaa acttggcccg tttgtggctg atattcatcg gtggcaaaat
      421 ctggatgact attacaacca gtactaccaa cgcgtaattg ttttgctttc tcatcctgcc
      481 aacccgcgag atcacaccaa cgttttgatg cacgttcagg gttattttcg cccgcatatt
      541 gattccacag aacgccagca gctggcagcg cttatcgaca gttatcgccg tggcgagcaa
      601 ccgcttcttg cgccgctgat gcgtatcaaa cactatatgg cgctttatcc tgacgcctgg
      661 ctttcagggc agcgttattt cgaactttgg ccgcgtgtga ttaacttgcg ccattcagga
      721 gttttatgac tacccatctg gtctggtttc gccaggattt acgtctgcac gataatctcg
      781 cgctggctgc cgcctgccgc aattcgtctg cacgcgtgct ggcgttgtat atcgctacac
      841 cacgccagtg ggcggcgcat aatatgtcgc cgcgtcaggc tgaacttatc aatgctcaac
      901 tgaacgggct acaaatcgcg ctggcggaaa aaggtattcc tttattgttc cgtgaagtgg
      961 atgactttgt cgccagcgtc gaattagtta aacaggtgtg cgcggaaaac agcgttactc
     1021 atctttttta taactatcag tatgaggtga atgagcgggc gcgggatgtg gacgttgaaa
     1081 gagcgctgcg taacgtggtg tgtgaaggat ttgatgacag cgtgatcctg ccgccggggg
     1141 cggtgatgac tggcaatcac gagatgtaca aagtctttac gccttttaag aatgcctggc
     1201 tgaaacggct gcgggaaggg atgccggagt gcgtcgctgc accaaaagtt cgtagtagcg
     1261 gatcgataaa gcccgcgcca tccattacgc tgaattatcc tcgtcagtct ttcgatactg
     1321 cgcatttccc ggtggaagaa aaagcggcga ttgcgcaatt acgccagttt tgccagaacg
     1381 gtaccggaga gtatgagcaa caacgagatt ttccggcagt ggaaggcacc agccgtttgt
     1441 ccgccagcct ggcaacgggc gggttatcac ctcgccagtg tttgcatcgc ctgttggcgg
     1501 aacagccgca ggcgctggac ggtggggccg gtagtgtctg gcttaatgag ctgatctggc
     1561 gcgagtttta ccgtcacctg ataacgtatc acccctcgtt gtgtaaacat cgtccattta
     1621 ttgcctggac ggatcgtgtg cagtggcaga gcaatcccgc acatttacag gcctggcagg
     1681 aaggcaaaac gggatacccg attgtcgatg ctgccatgcg tcagcttaac agcagtggct
     1741 ggatgcataa caggctacgg atgattacag ccagttttct ggtgaaagat ttgttgatca
     1801 actggcgcga aggcgagcga tatttcatgt cgcagctgat tgatggtgat ttggcagcca
     1861 ataacggtgg ctggcagtgg gcggcttcta cgggtactga tgcagcgccg tattttcgta
     1921 ttttcaaccc gacaacccag ggcgagaaat ttgaccgcga aggcgagttt atccgccagt
     1981 ggctaccgga actgcgcaat gtaccgggga aatcggtgca tgagccgtgg aagtgggctg
     2041 agaaagcagg tgtgaagctg gattatccgc aaccgatagt cgagcacaaa gaggcgagag
     2101 tacaaacgtt ggcagcgtat gaggcggcgc ggaaggggaa ataacagacg cgtcaggcaa
     2161 tcgagcccag atgccggatg cggcgtgaac gccttatcca gcctacaaat ttgtgcaaat
     2221 tcaataaatt gcaggaaata cgtaggcctg ataagacgcg tcaagcgtcg catcaggcat
     2281 cggtgctggc ctgttaagac tccagcgcca gcgcgcggtt tctgaatttc agcgcctgat
     2341 acagccaaat catcagtacc actcctacac acgccagtgc gccccaggta atttgatcaa
     2401 atacttcaat atatgcattg atggagtagt tgatcgcccc ggaagcatca aacgaagcct
     2461 gcgatgtctg atcggcaatc acgcccgcca gatagttcgc aatcgcgccg gaaagcagca
     2521 tataaatgcc ggttaatacg ccggtcacac cggggatttc aatgcgcgta atctgcgaca
     2581 tagcaaccgg gtcgataaac agttccgcaa agcccatcac cgccaggcct aataccatca
     2641 gtggcagaga agagtgacca tacattgcgg accagcgggc gcttaaggtc agaatgcaga
     2701 atccggcgct catcaggcca aggccaagag caaattttcc ccagatgcgc acggtacgat
     2761 tacccgcgac actctctttt accacccacg ccaggaacac accgcacagc ataactgcga
     2821 aggcattaat cgactggaac atcgcggtcg gaacggtata accaaacata tcgcggttaa
     2881 cgaagcggtc gatataaagg ctaatcgagc taccgccctg ttgtgcgaag gcccagaaca
     2941 acatactgaa gaaggtgagc gtcacaatca gccccagctc cttacgctgt ttctggtttt
     3001 ctgctttgcg ataaattttt gccagtacac ccaggccaat gatagtcgcg acaatcaagg
     3061 cgtataccga ccattctttc cagaacagta cggtaatcag cgcaggcgtt gccaccagca
     3121 gaaccagcag ccatccccag ttcggcagca gaaagtttgt cgcacgcagt acttttttgt
     3181 taacgccgcg ggtatgtgtg aaatgacgat tgccacataa gaaaataacc agacccgcga
     3241 tcatacccac cgccgccagg ccaaagccca tcgcccaact gtactcttcc tgggcgtagc
     3301 cacaggcaat aggtgcgata atagacccca cgttacccgc tgcatacatc agcgagaaac
     3361 cgccatcacg acgcggatcg gttggctcat acagctcgcc gagcaggcaa ctgacattgg
     3421 atttaaacag accatagcca caaacgatta tcgccaggga cagatagagg aatgacggat
     3481 ggatctcact tgcacccaac accacatgac cgatcgccat caataacgcc cccagcatca
     3541 ccgccatgcg attgccgaga actttatccg ccaaaaagcc accgaggatt ggcgtgacat
     3601 acaccagcga acagtaggcg ctaaataact cgtaggcgtg agtatcgttg tattttagtt
     3661 gattggtgag atagagaatc agcagggcac gcatgccgta aaagctaaaa tattcccaga
     3721 tttgcagcgc aacgacatag taaatagcgc gcggttgtga tgcgtgttta ttcataataa
     3781 ttccacaatt gacggcaacg tcacgtaata gttgcgcggc gtgcttgtgt ttccttaaag
     3841 ttaaaacttt gatacagatc tgattattta tgcaatatgc tgtctgattg cataaatata
     3901 cattagctga agcgtgatga tataaacaat ttgcgtcttt tggcgtcggg tttgtttctt
     3961 aatatgtcaa aagattggct atcgacgaac aggcgaggta acgctatgtt aacggtgtag
     4021 ctatcagaat acggaaggcg cgaagatgaa aaacaccgaa ctggaacaac tgattaacga
     4081 aaaactgaac agcgcggcga ttagtgatta cgcgccgaat ggtttgcagg tggaaggcaa
     4141 agagacggtg caaaaaattg ttaccggtgt caccgccagc caggctttgc tcgatgaggc
     4201 agtgcgtctg ggcgcggatg cggtcattgt gcatcatggc tacttctgga aaggcgaatc
     4261 tccggtcatt cgcggaatga agcgtaaccg tttaaaaacg ttgctggcga atgatatcaa
     4321 cctgtatggc tggcatttgc cgcttgacgc acatcctgag ctgggcaata acgcacagct
     4381 ggcggcgtta ctggggatca cggtcatggg cgaaattgag ccgttggtgc cgtggggcga
     4441 actgaccatg cctgtgccgg gactggagct ggcttcctgg attgaagcgc gtctgggacg
     4501 taagccgtta tggtgtggcg ataccggacc tgaggtggtc cagcgcgtcg cctggtgcac
     4561 gggcggcggg caaagtttta tcgatagcgc cgcgcgtttt ggcgtggatg cttttattac
     4621 tggcgaagtt tctgaacaga ccattcattc agcccgcgag cagggattgc atttttatgc
     4681 tgcaggtcac catgccactg aacgtggtgg tattcgcgca ttgagcgagt ggctgaatga
     4741 aaataccgat cttgatgtga cctttattga tattcctaat cctgcataac gaataatcag
     4801 agggatcgaa agtgcaacga gcgcgttgtt atctgatagg tgaaacggcg gtagtgctgg
     4861 aactggaacc gccggtgacg ctggctagcc agaaacggat ctggcgactg gcgcagcgtc
     4921 tggtggatat gccgaatgtg gttgaagcca ttcccggcat gaacaatatc acggtgattt
     4981 tgcgtaatcc tgagtcgctg gcgctggatg ccatagagcg tttgcaacgc tggtgggagg
     5041 agagcgaggc cctggaacct gagtctcgct ttattgaaat tccggtggtt tacggtggtg
     5101 caggcggacc ggatttggcg gtggtcgcgg cgcattgcgg gttgagcgaa aaacaggttg
     5161 ttgaattgca ctcctccgtg gaatacgtgg tctggttttt aggttttcaa ccgggcttcc
     5221 cgtatctcgg gagtttgccg gaacaactac acacgccacg gcgcgctgaa ccgcgcttgc
     5281 tcgttccggc aggttctgtc gggatcggcg ggccgcagac tggtgtttat ccgctggcaa
     5341 cgccgggtgg ctggcagttg attggtcata cctcactcag cctgtttgat ccggcgcgtg
     5401 acgaacccat cttattacgt ccgggagaca gcgtgcgctt tgtaccgcaa aaggagggag
     5461 tatgctgaag attattcgtg cgggcatgta taccactgtg caagatggcg gtcgtcacgg
     5521 ttttcgccag tcgggtatca gccactgcgg cgcactggat atgcctgcgt tacgcattgc
     5581 taacctgctg gtgggtaatg atgccaatgc ccccgcgctg gagatcacgc tcggtcagtt
     5641 aacggttgag ttcgaaactg atgggtggtt tgctctgacg ggtgccggtt gcgaagcgcg
     5701 gctggatgat aatgccgtct ggaccggctg gcgattgccg atgaaagcag gccagcgttt
     5761 aacgcttaaa cgcccgcagc acgggatgcg cagttatctg gcggtcgcgg gtggtattga
     5821 tgttccgccg gtaatggggt catgcagcac cgatctcaaa gtggggattg gcgggctgga
     5881 aggccgttta ctgaaggatg gtgaccgact cccgattggc aaatcgaagc gtgattttat
     5941 ggaagcgcag ggcgttaaac agctgctgtg gggcaaccgc attcgcgcct tgccggggcc
     6001 ggaatatcat gagttcgatc gcgcctcgca ggatgcattc tggcgttcgc cctggcagct
     6061 tagctcgcaa agtaaccgca tgggctatcg cttacagggg caaattttaa aacgcaccac
     6121 cgatcgcgaa ctgttatctc acggtttgtt accgggcgtg gtgcaggtgc cgcataacgg
     6181 gcagcccatt gtgttgatga acgacgcaca gaccaccggt ggttacccgc gtattgcctg
     6241 tatcattgag gctgatatgt accatctggc gcaaattccg ctcggtcagc cgattcattt
     6301 tgtccagtgt tcactggaag aggcactgaa agcgcggcaa gatcagcaac gttatttcga
     6361 acaattagcg tggcggctgc acaatgaaaa ttgacctgaa cgccgatctg ggcgaaggct
     6421 gcaccagcga cgcagagcta ttaacgctgg tttcctctgc caatattgcc tgtggatttc
     6481 atgcaggcga tgcgcaaacc atgcaggctt gcgtgcgtga agcaataaaa aatggtgtcg
     6541 cgattggcgc tcatccgagt tttcccgaca gggaaaattt tggtcgcagc gctatgcagc
     6601 tgccgccaga aaccgtttac gcccagacgc tgtatcaaat tggcgcgctg gcaacgattg
     6661 cccgtgcgca aggcggcgta atgcgtcatg tcaaaccgca cggcatgttg tacaaccagg
     6721 cggcgaaaga agcacaactg gcagacgcca tcgccagagc ggtatacgct tgcgatccag
     6781 cattggttct cgttgggctg gcaggaagcg agctgattcg tgcaggcaag caatatggtc
     6841 tgacaacgcg cgaggaagtg tttgccgatc gcggttatca ggctgacggc tcgctggtgc
     6901 cgcgaagcca gtcaggcgcg ttgattgaaa acgaagaaca ggcgctggcg caaacgctgg
     6961 agatggtgca acacggcaga gtcaaaagta tcaccggcga atgggcaacg gtcacggcgc
     7021 aaacggtctg cctgcatggc gacggcgagc acgcactggc attcgcccgc cgactacgct
     7081 ctacatttgc cgaaaagggg attgttgtcg cagcataacc ccgtttaata aagaatgaaa
     7141 aaaggatatc accatgcctg aaggcccgga gatccgccgt gcagcggata acctggaggc
     7201 ggcgatcaaa ggcaaaccac taactgatgt ctggtttgcc ttcccgcagt taaaaactta
     7261 tcaatcacaa cttatcggtc aacacgttac ccatgtggaa acgcgtggta aggcgttgtt
     7321 aactcatttt tccaacgact taacgctcta cagccataat cagctttatg gcgtctggcg
     7381 cgtggttgat accgacgaag agccgcagac cacgcgagta ctgcgggtaa aactgcaaac
     7441 ggctgacaaa actattctgc tttatagcgc ctcggatatt gagatgttga ccccggaaca
     7501 actgaccacg catccgtttt tacaacgcgt tggtcccgat gtgctggatc cgaatctgac
     7561 gccggaggtg gtgaaagaac ggttattgtc gctgcgcttt cgtaaccgtc agtttgctgg
     7621 attactgctc gatcaggcgt ttctggctgg gcttggcaat tatttgcgtg tggagatcct
     7681 ctggcaggtt gggttgactg gaaatcataa agcgaaagac ctcaatgcgg cgcaactgga
     7741 tgcactcgca cacgcgttac tggatattcc tcggttttct tacgctacgc gggggcaggt
     7801 ggatgagaat aagcatcatg gggcgctgtt tcgctttaag gtttttcatc gtgatggcga
     7861 actgtgcgaa cgctgtggcg gcatcattga gaaaaccacg ctgtcatctc gcccgtttta
     7921 ctggtgtccg ggctgccagc actaggccgt acgtttcggc gcataggttg aaataaaccg
     7981 cgcaatggca ggccctgtca gcaaaatact gaacaggcgc agggtttgca tcgccataat
     8041 gagcgccata tcggcattgc tccctgcggc gatgaccgcc acggtatcaa gcccgccggg
     8101 gctggtggcg aggtaggcag tcataaaatc aatgtgcata aaccgggtca gcccccacgc
     8161 catacccgca caaatagcca gcagagcaaa aatcgacagc aggatttgtg gtagcgggcg
     8221 tagtgcccgc agtaagattt gtttatcgaa accaagacca atccgccagc caattgccat
     8281 atacgccatc gccagcagcc attccggtag ttcgatggtg atgagctgac cagactggag
     8341 cactgcgccc gccagcatcg ggatgagcat cgtgccggaa ggaagacgca gcaggcatcc
     8401 cactgtaccg gcaacgaccg cgagcaaaat agtaagcagg agattaatgc tcactggcgg
     8461 gaaccagacg atatgctggt taaccgcttc agcgttatcg cccagcatca ttcgcgtgac
     8521 cagaactgcg gcccccgcga caaacagcac tcgcagatac tgcataaacg ccaccaggcg
     8581 gatatctgcg ccgtaatcct gcgccatcgc gaccattgcc gcagccccgc caggcgagga
     8641 gccccaggca ccagtatttc cgggcagtga gctatagcgc accagtagcc atcccacgat
     8701 agcgctggaa agcaacgtca ccagtaaaat agctaacacg atcggccagt taaccgccag
     8761 cgtggtcagg atcgaaccgg tcaagttttg cgcaatcatg cagccaagaa tggcctgcgc
     8821 ggcgagaaaa gcggagcggg ggagttgcag ggtaaccccg cgcatactga agatgatccc
     8881 ggcgatcatc ggcccaagta ataatgccgc cgggagatgt accgcgagga aaccaataga
     8941 aaggagaagt gatagcacac ataacattcc ccactgcaaa actggcatcc tttgctccct
     9001 cattattttc tctttggtgt atcgatacag cataaagaat gccgggcatg gaaaaaataa
     9061 cggtaatcaa taaaagccgg aaaattggca attaaccgaa gaagccctct ccggctggaa
     9121 agggcaatag gctaaatcag ttgtagttaa ttttgaaaag taccactgta tcaaagggac
     9181 caacgtcgat gggcgtaccc ggcgtggatg acaattctgc cgtgtaggtt ttttcataca
     9241 ctaacaggtc gctggtaaaa tcggcatact cactgtattt gttgaaagta tagggtgagg
     9301 cattttcatc caacaatcga agcttcaggc cattgccaat gagcagtgat ttatcttcgt
     9361 cgataagggt ttcatcggta taaaacgaag agctgacttt aaaaccgtcg gtacactggt
     9421 cgttttgtga tttggttgta gttattttaa attgctgcga catcgtatgg cgacgaatat
     9481 ccagcacgtt aaatcggcca aaatcaacga cctgggtttc aggtgagata gcaaagttga
     9541 cgctgcaatc cagcacgcgg atattttcca gaccggtaat gtgatatttc agattcttcg
     9601 cggtagggtc ttcgttcacg ctgcctgcgc catcaaactg tacgacgatg taatcgctga
     9661 gtttgctctg ataatcatgg ggcggcatgg cgcgaacttt aacgtaaagc cgcatacgcg
     9721 ccacaaacgt ccgcgatttg tgcataacgg ttggatcgcc ggagcaaagt ttattttgcc
     9781 agcccatcgc aatgatctgc tccggggtgt aaatatcaat attcttactg tcgatgcact
     9841 gattcgtgtc gatgcggctt ttcccaaggc ttgcgtcata gtccacgcct tcataggtta
     9901 cgcccaattg ataataggga tcctgaatac cggggtaagg gtttacccag gcgtagacgt
     9961 gctcgctttc aaaattaccg tttgtgttgt tgtcacagta gaccggaatt tttatatcgt
    10021 cagattccca gattttatcg ccgggtttgg aattggccgg aacggcgaag ggcataatag
    10081 cttccgattt ttctacagcg ccgtttgcgg taccaaaata gcagttcagc gccagcgccg
    10141 cttttagcgg caaaaaaatc agacagatgg cgagcaacac gcctttggcg aatctcattt
    10201 tttattctcc accggctgca ggcggcattg ccgctggcag gagagcgtgt tcatacgcaa
    10261 tgcgccgtag tcgtccatgt agccaagata aaactgcttg ccgctatagc tgcccgtgct
    10321 ggtggtgacc gaactgaacg gtgcgaccat cgtgctttta aagccaggca gcacgccttt
    10381 ctcatcccgc ccaagatagg caatagtcag atagtatccg gtcgggtttt tcagggtaag
    10441 ctggttattc tgctggctaa cctgtagctg ttgctcaaca tgatcgccca tttttttgcg
    10501 taatgcggcg ggccgccaga atagcttaat gcgactttgg acggcgactt gcaggatcgc
    10561 atggccctcg ctgccttccg ggacaggcgg aatttcacgc aggttatagt aaaagagaga
    10621 ttcgcgatcg gtgggtaatt tcgcggtggc ggcttgtttc atgatccgaa cctgcgaagt
    10681 cgcttttggc tccagtcgct ggataggcgg cagggcgacg agaaattcat cgcttttctc
    10741 tcccttttca ttttctatcc atgaataggc gaggtagggg agtttatcgc tgcggttttc
    10801 cacgcgcagg ctggttgctt tatctttgct attaaagatg atgcgcgtgc ggtcaggctg
    10861 cactgcgccg tggctgcaca tactggcaac cagaagaatc aaagggaatc ctttaacaaa
    10921 tgtcatcgtc ttttccttaa ttaatgacag ggtaaaatca ggcgattagt cacatcttcc
    10981 agatgttccg gcagatgaat ggcacacgtt ttctggtcgc cccagtgcac ggtaaattgt
    11041 tgattttcat cgaccccact cagccatgcg tgaccgtttt cgccaaccat cccgacgctg
    11101 acaccgcctt cagcctggcg aacatcggca ccgagcggcg ggaaatgccc gttagcatcg
    11161 ctgataatga cattgagatc tttcccggcg cgggacgcca gcgatttaaa gccgatggcg
    11221 ccttcggtcc aggtttcttt caccacgttt tcggaaacgg taacgccgtc aggcaagtcg
    11281 ttcatattga ccgctaccgt cgtaggctgg tagctggaaa caaacggtac cacggcaata
    11341 ccaaagcggt tggtgtagtc aatattgccc tgaatcggaa tatcgcccac gccgtcggtg
    11401 ctgaccatca ggcgtggttc attggtggaa ctgcggcgat ggaatgccgc gccatgttga
    11461 gtcgcggtaa acgagccgct ccaactggca ctggcggaag tgtaatcatt ggcggcatag
    11521 gttccggtca gatcccattc ccccgccgaa ctgaggtgtt ggtaattgcc gctgacctgc
    11581 gcaccgttat ccgggcgatc ggattgtatt ccagccgaca ttccccaact gtttcgttca
    11641 tccagggtat cgttccacga catgcggtgg gtggtagtat tactgttgtt ctgcatgtca
    11701 taacccagtc gaccactttc cccaatcggt aaggagatcg aaaagtaaat ctggttgtcg
    11761 cgatctttat cttcataatg ggtggtgttg aacgaggtcg agacagaaat atctttccag
    11821 tcgccaatgt cgacgttaaa accgacggta atgttggccg tggtggaggt atccgcattc
    11881 caccagctct gatgcaggat gttggcgtag agattaagat tcagcggagt aataggctga
    11941 ccgaaggaga ggctgatggt ttgcttttca tcctgcgcgt cggcgtcatt gtatttgtga
    12001 tcaatgtagt tagcgtagct gtgaaaatgg cgatcggaga agcgataggc tgccaatgag
    12061 atggtgctgt tggtcgcatc aacctgtttg ctgtagttaa agcggtagct gtaaccctgc
    12121 tcatcctgtt gagtatcaaa atggctgtcg gcccaggtca cgtcaaacga cagcgccccc
    12181 agccagagca tattttgccc aatacccagc gcgccggaac gatagtcatc gcctgcgaga
    12241 agcatgccgc catatagtga agtgttggag agcattcccc aggagacttc atggctaatg
    12301 aaggtttcat cctcggtgtg gtgcgacata gaggaacgcg ggcgaccggc agccagttta
    12361 tagcgcacct ggccctgacg cgtcaggaac ggggtagagg ccgctgaaac ctggaagtta
    12421 ttcacccgtc catcttcttc gctcactttg acatccagtg taccttgcac tgactgattc
    12481 aggtcatcaa taataaatgg gcctggcgga acttttttct ggtaaatcac gcgaccagaa
    12541 tggcttatcg tgaccgtggc gttagtctgt gcgatgccgc tgatttgcgg cgcgtagccg
    12601 cgtaactccc aggggagcat tcgatcgtcg ctggctaacg ccgcaccggt ataggagaaa
    12661 ccatcaaaaa tgttagagct aaaatcggtt tcgcccaggg ttaatcggga acctatttgc
    12721 ggtaacgggc ggaacaggta ggtccgtgaa atcgccccgg attgttcacg gttgtcgccg
    12781 ctatcggact ggctaagctg ataatcgctg cgcagacgcc atgccccggc gttgaggccc
    12841 gtggtgccat aggcgttcag gttgttgctg ctgctgccgc tttgtgggcg ataagtgctg
    12901 gcaaacaggt tgtaatccat caggaagcca ggaataccgt tattccacgt tgagggtggc
    12961 gtccagtttt ctgaatgcca cgccagccat gcctgcggga ttgtaatatt gagctgttga
    13021 tttgcctgat cgaaagtaaa gatgatttca gggaagacac taaaatcgac acattctttt
    13081 attgccggta attttttacg aaagtcagat ttaagtccga agagttcgat cagcgattca
    13141 ttgatacaag gaataatttt atcgccagat ttttgccagc gaatttgttg cccattactg
    13201 attttattat tattaaccgt aacactaaca aagtaatcgc cgggagctac gacgcctttt
    13261 tgattgagca acgaaatatc aacgctatcc cgaatcgact tatcgagaac gttaaggtta
    13321 aattcagctg ccagcgcagg aagggttacc attgccagac aggaaagaac agagagtcga
    13381 taaatattca cggcatccat acctgaacaa tattcgcaaa aggcggctaa tcagccgcct
    13441 taaaaaggat tattggtaat ccagaacgta agttgcgtta gcagtcacgg cccccgcggt
    13501 aaccgaaact gcgttatcca tctgttccat ccaggcaaag aagtttaacg tttgttctgt
    13561 tgaacttgcc aataagtcaa ttgttggcgc ttctgttccc aatacaacaa atgattcatc
    13621 tttgttcatg agacgtacgc caacgccggt agcctcgcca gcactgatgt tactaaggag
    13681 cggcgttgca cctgtcgttt tagctgtgct gtcgaaggtc acgcctacct ttgagaccac
    13741 gccaccgttg ccatcatccc agcctgcaat atcacagttg attaagcgaa tattgacagg
    13801 ttttttctcg ctatgatgtt ctcgatttat gtgggatgtg gttacttcac ccaggtcaat
    13861 gttcatatca aggtcatcag ggtgaatttc acaaggtgct tcaataactt cacctttaaa
    13921 gtggatttca ccagatcctt catctgcagc aaaaacaggt gaagcgaata gtagagatat
    13981 ggccagggcg gccagtgttt tttgtccttt aaacatacat gaatccttca tgggctatga
    14041 taaagtatac taattattcc gacagaatag tggttttttg ctcatgtaat aatattaatg
    14101 ttgctacatt atgtttgaga gtttctcaaa gctctcgaat cataaataaa gtgaattaat
    14161 cctattacga gtttatttaa ttgtgggtgc tacccaatgt attctacatc gattggcggg
    14221 ataacggtgg tggtgaatga aaaattagga ttttatttag ttatcggata tgtattcttg
    14281 taacgtgtaa ttatgtattt gtgtccgatt acgttttttt gtcgatagac ataactaacg
    14341 aacaaaaaaa agcccgccag atattcaggc gggctaaacg atatatggtt caaatcagat
    14401 aattaatgtt taacatctga tttaaagtct cgtttttcat agccggtgta caactggcgc
    14461 ggacgggcga tcttcatgcc gtcactgtgc atttcgctcc agtgagcgat ccagccaacg
    14521 gtacgggcca tagcgaagat gacggtgaac atggaagacg gaatacccat cgctttcagg
    14581 atgataccag agtagaaatc gacgttcggg tacagtttct tctcgataaa gtacgggtcg
    14641 ttcagcgcga tgttttccag ctccatagcc acttccagca ggtcatcttt ggtgcccagc
    14701 tctttcagaa cttcatggca ggtttcacgc attacggtgg cgcgcgggtc gtaatttttg
    14761 tacacgcggt gaccgaagcc catcaggcgg aaagaatcat ttttatcttt cgcacgacga
    14821 acaaattccg gaatgtgttt aacggagcta atttcttcca gcattttcag cgccgcttcg
    14881 ttagcaccac cgtgagcagg tccccacagt gaagcaatac ctgctgcgat acaggcaaac
    14941 gggttcgcac ccgaagagcc agcggtacgc acggtggagg tagaggcgtt ctgttcatgg
    15001 tcagcgtgca ggatcagaat acggtccata gcacgttcca gaatcggatt aacttcatac
    15061 ggttcgcacg gcgtggagaa catcatattc aggaagttac cggcatagga gagatcgttg
    15121 cgcggataaa caaatggctg accaatggaa tacttgtaac acatcgctgc catggtcggc
    15181 attttcgaca gcaggcggaa cgcggcaatt tcacgatgac gaggattgtt aacatccagc
    15241 gagtcgtgat agaacgccgc cagcgcgccg gtaataccac acatgactgc cattggatgt
    15301 gagtcgcgac ggaaagcgtg gaacagacgg gtaatctgct cgtggatcat ggtatgacgg
    15361 gtcaccgtag ttttaaattc gtcatactgt tcctgagtcg gtttttcacc attcagcagg
    15421 atgtaacaaa cttccaggta gttagaatcg gtcgccagct gatcgatcgg gaaaccgcgg
    15481 tgcagcaaaa taccttcatc accatcaata aaagtaattt tagattcgca ggatgcggtt
    15541 gaagtgaagc cagggtcaaa ggtgaacaca ccttttgaac cgagagtacg gatatcaata
    15601 acatcttgac ccagcgtgcc tttcagcaca tccagttcaa cagctgtgtc cccgttgagg
    15661 gtgagttttg cttttgtatc agccatttaa ggtctcctta gcgccttatt gcgtaagact
    15721 gccggaactt aaatttgcct tcgcacatca acctggcttt acccgtttgt tatttggctc
    15781 gccgctctgt gaaagagggg aaaacctggg tacagagctc tgggcgcttg caggtaaagg
    15841 atccattgat gacgaataaa tggcgaatca agtacttagc aatccgaatt attaaacttg
    15901 tctaccacta ataactgtcc cgaatgaatt ggtcaatact tccacactgt tacataagtt
    15961 aatcttaggt gaaataccga cttcataact tttacgcatt atatgctttt cctggtaatg
    16021 tttgtaacaa ctttgttgaa tgattgtcaa attagatgat taaaaattaa ataaatgttg
    16081 ttatcgtgac ctggatcact gttcaggata aaacccgaca aactatatgt aggttaattg
    16141 taatgatttt gtgaacagcc tatactgccg ccaggtctcc ggaacaccct gcaatcccga
    16201 gccacccagc gttgtaacgt gtcgttttcg catctggaag cagtgttttg catgacgcgc
    16261 agttatagaa aggacgctgt ctgacccgca agcagaccgg aggaaggaaa tcccgacgtc
    16321 tccaggtaac agaaagttaa cctctgtgcc cgtagtcccc agggaataat aagaacagca
    16381 tgtgggcgtt attcatgata agaaatgtga aaaaacaaag acctgttaat ctggacctac
    16441 agaccatccg gttccccatc acggcgatag cgtccattct ccatcgcgtt tccggtgtga
    16501 tcacctttgt tgcagtgggt atcctgctgt ggcttctggg taccagcctc tcttcccctg
    16561 aaggtttcga gcaagcttcc gcgattatgg gcagcttctt cgtcaaattt atcatgtggg
    16621 gcatccttac cgctctggcg tatcacgtcg tcgtaggtat tcgccacatg atgatggatt
    16681 ttggctatct ggaagaaaca ttcgaagcgg gtaaacgctc cgccaaaatc tcctttgtta
    16741 ttactgtcgt gctttcactt ctcgcaggag tcctcgtatg gtaagcaacg cctccgcatt
    16801 aggacgcaat ggcgtacatg atttcatcct cgttcgcgct accgctatcg tcctgacgct
    16861 ctacatcatt tatatggtcg gttttttcgc caccagtggc gagctgacat atgaagtctg
    16921 gatcggtttc ttcgcctctg cgttcaccaa agtgttcacc ctgctggcgc tgttttctat
    16981 cttaatccat gcctggatcg gcatgtggca ggtgttgacc gactacgtta aaccgctggc
    17041 tttgcgcctg atgctgcaac tggtgattgt cgttgcactg gtggtttacg tgatttatgg
    17101 attcgttgtg gtgtggggtg tgtgatgaaa ttgccagtca gagaatttga tgcagttgtg
    17161 attggtgccg gcggcgcagg tatgcgcgcg gcgctgcaaa tttcccagag cggccagacc
    17221 tgtgcgctgc tctctaaagt cttcccgacc cgttcccata ccgtttctgc gcaaggcggc
    17281 attaccgttg cgctgggtaa tacccatgaa gataactggg aatggcatat gtacgacacc
    17341 gtaaaagggt cggactatat cggtgaccag gacgcgattg aatatatgtg taaaaccggg
    17401 ccggaagcga ttctggaact cgaacacatg ggcctgccgt tctcgcgtct cgatgatggt
    17461 cgtatctatc aacgtccgtt tggcggtcag tcgaaaaact tcggcggcga gcaggcggca
    17521 cgcactgcgg cagcggctga ccgtaccggt cacgcactgt tgcacacgct ttatcagcag
    17581 aacctgaaaa accacaccac tattttctcc gagtggtatg cgcttgatct ggtgaaaaac
    17641 caggatggcg cggtggtggg ttgtaccgca ctgtgcatcg aaaccggtga agtggtttat
    17701 tttaaagccc gcgctaccgt gctggcgact ggcggggctg ggcgtattta tcagtccacc
    17761 actaacgccc acattaacac tggcgacggt gtcggcatgg ctatccgcgc aggcgtaccg
    17821 gtgcaggata tggaaatgtg gcaattccac ccgaccggca ttgccggtgc gggcgtactg
    17881 gtcaccgaag gttgccgtgg tgaaggcggt tatctgctga acaaacatgg cgaacgcttt
    17941 atggagcgtt atgcgccgaa cgccaaagac ctggcgggcc gtgatgtggt ggcgcgttcc
    18001 atcatgattg aaatccgtga aggtcgcggc tgtgatggtc cgtgggggcc acacgcgaaa
    18061 ctgaaactcg atcacctggg taaagaagtt ctcgaatccc gtctgccggg tatcctggag
    18121 ctttcccgta ccttcgctca cgttgatccg gtgaaagagc cgattccggt tatcccaacc
    18181 tgtcactaca tgatgggcgg tattccgacc aaagttaccg gtcaggcgct gactgtgaat
    18241 gagaaaggcg aagatgtggt tgttccgggg ctgttcgcgg taggtgaaat tgcctgcgta
    18301 tcggtacacg gtgctaaccg tctgggcggc aactcgctgc tggacctggt ggtctttggt
    18361 cgcgcagcgg gtctgcatct gcaagagtct atcgccgagc agggcgcatt gcgcgatgcc
    18421 agcgagtctg atgttgaagc atctctggat cgtctgaacc gctggaacaa taatcgtaac
    18481 ggtgaagatc cggtggcgat tcgtaaagcg ctgcaagaat gtatgcagca taacttctcg
    18541 gtcttccgtg aaggtgatgc gatggcgaaa ggtcttgagc agttgaaagt tatccgcgag
    18601 cgtctgaaaa atgcccgtct ggatgacact tccagcgagt tcaacaccca gcgcgttgag
    18661 tgtctggaac tggataacct gatggaaacg gcgtatgcaa cggctatttc tgccaacttc
    18721 cgtaccgaaa gccgtggcgc gcatagccgc ttcgacttcc cggatcgtga tgatgaaaac
    18781 tggctgtgcc actccctgta tctgccagag tcggaatcca tgacgcgccg aagcgtcaac
    18841 atggaaccga aactgcgccc ggcattcccg ccgaagattc gtacttacta atgcggagac
    18901 aggaaaatga gactcgagtt ttcaatttat cgctataacc cggatgttga tgatgctccg
    18961 cgtatgcagg attacaccct ggaagcggaa gaaggtcgcg acatgatgct gctggatgcg
    19021 cttatccagc tgaaagagaa agatcccagc ctgtcgttcc gccgctcctg ccgtgaaggt
    19081 gtgtgcggtt ccgacggtct gaacatgaac ggcaagaatg gtctggcctg tattaccccg
    19141 atttcggcac tcaaccagcc gggcaagaag attgtgattc gcccgctgcc aggtttaccg
    19201 gtgatccgcg atttggtggt agacatggga caattctatg cgcaatatga gaaaattaag
    19261 ccttacctgt tgaataatgg acaaaatccg ccagctcgcg agcatttaca gatgccagag
    19321 cagcgcgaaa aactcgacgg gctgtatgaa tgtattctct gcgcatgttg ttcaacctct
    19381 tgtccgtctt tctggtggaa tcccgataag tttatcggcc cggcaggctt gttagcggca
    19441 tatcgtttcc tgatcgatag ccgtgatacc gagactgaca gccgcctcga cggtttgagc
    19501 gatgcattca gtgtattccg ctgtcacagc atcatgaact gcgtcagtgt atgtccgaag
    19561 ggactgaacc cgacgcgcgc catcggtcat atcaagtcga tgttgttgca acgtaatgcg
    19621 taaaccgtag gcctgataag acgcgcaagc gtcgcatcag gcaaccagtg ccggatgcgg
    19681 cgtgaacgcc ttatccggcc tacaagtcat tacccgtagg cctgataagc gcagcgcatc
    19741 aggcgtaaca aagaaatgca ggaaatcttt aaaaactgcc cctgacacta agacagtttt
    19801 taaaggttcc ttcgcgagcc actacgtaga caagagctcg caagtgaacc ccggcacgca
    19861 catcactgtg cgtggtagta tccacggcga agtaagcata aaaaagatgc ttaagggatc
    19921 acgatgcaga acagcgcttt gaaagcctgg ttggactctt cttacctctc tggcgcaaac
    19981 cagagctgga tagaacagct ctatgaagac ttcttaaccg atcctgactc ggttgacgct
    20041 aactggcgtt cgacgttcca gcagttacct ggtacgggag tcaaaccgga tcaattccac
    20101 tctcaaacgc gtgaatattt ccgccgcctg gcgaaagacg cttcacgtta ctcttcaacg
    20161 atctccgacc ctgacaccaa tgtgaagcag gtaaaagtcc tgcagctcat taacgcatac
    20221 cgcttccgtg gtcaccagca tgcgaatctc gatccgctgg gactgtggca gcaagataaa
    20281 gtggccgatc tggatccgtc tttccacgat ctgaccgaag cagacttcca ggagaccttc
    20341 aacgtcggtt catttgccag cggcaaagaa accatgaaac tcggcgagct gctggaagcc
    20401 ctcaagcaaa cctactgcgg cccgattggt gccgagtata tgcacatcac cagcaccgaa
    20461 gaaaaacgct ggatccaaca gcgtatcgag tctggtcgcg cgactttcaa tagcgaagag
    20521 aaaaaacgct tcttaagcga actgaccgcc gctgaaggcc ttgaacgtta cctcggtgca
    20581 aaattccctg gtgcaaaacg cttctcactg gaaggcggtg acgcgttaat cccgatgctt
    20641 aaagagatga tccgccacgc tggcaacagc ggcacccggg aagtggttct cgggatggcg
    20701 caccgtggtc gtctgaacgt gctggtgaac gtgctgggta aaaaaccgca agacttgttc
    20761 gacgagtttg ccggtaaaca taaagaacac ctcggcacgg gtgacgtgaa ataccacatg
    20821 ggcttctcgt ctgacttcca gaccgatggc ggcctggtgc acctggcgct ggcgtttaac
    20881 ccgtctcacc ttgagattgt aagcccggta gttatcggtt ctgttcgtgc ccgtctggac
    20941 agacttgatg agccgagcag caacaaagtg ctgccaatca ccatccacgg tgacgccgca
    21001 gtgaccgggc agggcgtggt tcaggaaact ctgaacatgt cgaaagcgcg tggttatgaa
    21061 gttggcggta cggtacgtat cgttatcaac aaccaggttg gtttcaccac ctctaatccg
    21121 ctggatgctc gttctacacc gtactgtact gatatcggta agatggttca ggcaccgatt
    21181 ttccacgtta acgcggatga tccggaagcc gttgcctttg tgacccgtct ggcgctcgat
    21241 ttccgtaaca cctttaaacg tgatgtcttc atcgacctgg tgtgctaccg ccgtcacggc
    21301 cacaacgaag ccgacgagcc gagcgcaacc cagccgctga tgtatcagaa aatcaaaaaa
    21361 catccgacgc cgcgcaaaat ctatgctgac aagctggagc aggaaaaagt ggcgacgctg
    21421 gaagatgcca ccgagatggt taacctgtac cgcgatgcgc tggatgctgg cgattgcgtg
    21481 gtagcagagt ggcgtccgat gaacatgcac tctttcacct ggtcgccgta tctcaaccac
    21541 gaatgggacg aagagtaccc gaacaaagtt gagatgaagc gcctgcagga actggctaaa
    21601 cgcatcagca cggtgccgga agcggttgaa atgcagtctc gcgttgccaa gatttatggc
    21661 gatcgccagg caatggcagc cggtgagaaa ctgttcgact ggggcggcgc ggaaaacctc
    21721 gcttacgcca cgctggttga tgaaggcatt ccggttcgcc tgtcgggtga agactccggt
    21781 cgcggtacgt tcttccaccg ccacgcggtg atccacaacc agtctaacgg ttccacttac
    21841 acgccgctgc aacacatcca taacggccag ggcgcgttcc gtgtctggga ctccgtactt
    21901 tctgaagaag cagtgctggc gtttgaatac ggttatgcca ccgcagaacc acgcactctg
    21961 actatctggg aagctcagtt cggtgacttc gccaacggtg cgcaggtggt tatcgaccag
    22021 ttcatctcct ctggcgaaca gaaatggggc cggatgtgtg gtctggtgat gttgctgccg
    22081 cacggttacg aagggcaggg gccggagcac tcctccgcgc gtctggaacg ttatctgcaa
    22141 ctttgtgctg agcaaaacat gcaggtgtgc gtaccgtcta ccccggcaca ggtttaccac
    22201 atgctgcgtc gtcaggcgct gcgcgggatg cgtcgtccgc tggtcgtgat gtcgccgaaa
    22261 tccctgctgc gtcatccgct ggcggtttcc agcctcgaag aactggcgaa cggcaccttc
    22321 ctgccagcca tcggtgaaat cgacgagctt gatccgaagg gcgtgaagcg cgtagtgatg
    22381 tgttctggta aggtttatta cgacctgctg gaacaacgtc gtaagaacaa tcaacacgat
    22441 gtcgccattg tgcgtatcga gcaactctac ccgttcccgc ataaagcgat gcaggaagtg
    22501 ttgcagcagt ttgctcacgt caaggatttt gtctggtgcc aggaagagcc gctcaaccag
    22561 ggcgcatggt actgcagcca gcatcatttc cgtgaagtga ttccgtttgg ggcttctctg
    22621 cgttatgcag gccgcccggc ctccgcctct ccggcggtag ggtatatgtc cgttcaccag
    22681 aaacagcaac aagatctggt taatgacgcg ctgaacgtcg aataaataaa ggatacacaa
    22741 tgagtagcgt agatattctg gtccctgacc tgcctgaatc cgtagccgat gccaccgtcg
    22801 caacctggca taaaaaaccc ggcgacgcag tcgtacgtga tgaagtgctg gtagaaatcg
    22861 aaactgacaa agtggtactg gaagtaccgg catcagcaga cggcattctg gatgcggttc
    22921 tggaagatga aggtacaacg gtaacgtctc gtcagatcct tggtcgcctg cgtgaaggca
    22981 acagcgccgg taaagaaacc agcgccaaat ctgaagagaa agcgtccact ccggcgcaac
    23041 gccagcaggc gtctctggaa gagcaaaaca acgatgcgtt aagcccggcg atccgtcgcc
    23101 tgctggctga acacaacctc gacgccagcg ccattaaagg caccggtgtg ggtggtcgtc
    23161 tgacccgtga agatgtggaa aaacatctgg cgaaagcccc ggcgaaagag tctgcaccgg
    23221 cagcggctgc tccggcggcg caaccggctc tggctgcacg tagcgaaaaa cgtgtcccga
    23281 tgactcgcct gcgtaagcgt gtggcagagc gtctgctgga agcgaaaaac tccaccgcca
    23341 tgctgaccac gttcaacgaa gtcaacatga agccgattat ggatctgcgt aagcagtacg
    23401 gtgaagcgtt tgaaaaacgc cacggcatcc gtctgggctt tatgtccttc tacgtgaaag
    23461 cggtggttga agccctgaaa cgttacccgg aagtgaatgc ttctatcgac ggcgatgacg
    23521 tggtttacca caactatttc gacgtcagca tggcggtttc tacgccgcgc ggcctggtga
    23581 cgccggtact gcgtgatgtc gataccctcg gcatggcaga catcgagaag aaaatcaaag
    23641 agctggcagt taaaggccgt gacggcaagc tgacggttga agatctgacc ggtggtaact
    23701 tcaccatcac caacggtggt gtgttcggtt ccctgatgtc tacgccgatc atcaacccgc
    23761 cgcagagcgc aattctgggt atgcacgcta tcaaagatcg tccgatggcg gtgaatggtc
    23821 aggttgagat cctgccgatg atgtacttgg cgctgtccta cgatcaccgt ctgatcgatg
    23881 gtcgcgaatc cgtgggcttc ctggtaacga tcaaagagtt gctggaagat ccgacgcgtc
    23941 tgctgctgga cgtttagtag tttaagcttc acctgcactg tagaccgggt aagacattat
    24001 cgccttctcc ggcaattgaa gcctgatgcg acgcttaacg cgtcttatca ggcctacggg
    24061 aagcaccgat gtaggtcgga taaggcgctc gcgtcgcatc cgacaaacga tgcctgatgc
    24121 gacgcttaca cgtcttatca ggcctacggg gagcaccgat gtaggtcgga taaggcgctc
    24181 gcgccgcatc cgacaagcga tgccagatgc gacgcttaca cgtcttatct ggcctacggg
    24241 tgaccgacaa tacccggaag cgatacgaaa tattcggtct acggtttaaa agataacgat
    24301 tactgaagga tggacagaac acatgaactt acatgaatat caggcaaaac aactttttgc
    24361 ccgctatggc ttaccagcac cggtgggtta tgcctgtact actccgcgcg aagcagaaga
    24421 agccgcttca aaaatcggtg ccggtccgtg ggtagtgaaa tgtcaggttc acgctggtgg
    24481 ccgcggtaaa gcgggcggtg tgaaagttgt aaacagtaaa gaagacatcc gtgcttttgc
    24541 agaaaactgg ctgggcaagc gtctggtaac gtatcaaaca gatgccaatg gccaaccggt
    24601 taaccagatt ctggttgaag cggcgaccga tatcgctaaa gagctgtatc tcggtgccgt
    24661 tgtagaccgt agttcacgtc gcgtggtctt tatggcctct accgaaggcg gcgtggaaat
    24721 cgaaaaagtg gcggaagaaa ctccgcacct gatccacaaa gttgcgcttg atccgctgac
    24781 tggcccgatg ccgtatcagg gacgcgagct ggcgttcaaa ctgggtctgg aaggtaaact
    24841 ggttcagcag ttcaccaaaa tcttcatggg cctggcgacc attttcctgg agcgcgacct
    24901 ggcgttgatc gaaatcaacc cgctggtcat caccaaacag ggtgatctga tttgcctcga
    24961 cggcaaactg ggcgctgatg gcaacgccct gttccgccag cctgatctgc gcgaaatgcg
    25021 tgaccagtcg caggaagatc cacgtgaagc acaggctgca cagtgggaac tgaactacgt
    25081 tgcgctggac ggtaacatcg gttgtatggt taacggcgca ggtctggcga tgggtacgat
    25141 ggacatcgtt aaactgcacg gcggagaacc ggctaacttc ctggacgttg gcggcggcgc
    25201 aaccaaagaa cgtgtaaccg aagcgttcaa aatcatcctc tctgacgaca aagtgaaagc
    25261 cgttctggtt aacatcttcg gcggtatcgt tcgttgcgat ctgatcgctg acggtatcat
    25321 cggcgcggta gcggaagtgg gtgttaacgt accggtcgtg gtacgtctgg aaggtaacaa
    25381 cgccgaactc ggcgcgaaga aactggctga cagcggcctg aatattattg cagcaaaagg
    25441 tctgacggat gcagctcagc aggttgttgc cgcagtggag gggaaataat gtccatttta
    25501 attgataaaa acaccaaggt tatctgccag ggctttaccg gtagccaggg gactttccac
    25561 tctgaacagg ccattgcata cggcaccaaa atggttggtg gcgtaactcc aggtaaaggc
    25621 ggtactaccc acctcggcct gccggtgttc aacacagtgc gtgaagctgt tgctgctact
    25681 ggcgctaccg cttctgttat ctacgtacca gcaccgttct gcaaagactc cattctggaa
    25741 gccatcgacg caggcatcaa actgattatc accatcactg aaggcatccc gacgctggat
    25801 atgctgactg tgaaagtgaa actggatgaa gccggcgttc gtatgatcgg cccgaactgc
    25861 ccaggcgtta tcaccccggg tgaatgcaaa attggtatcc aaccgggtca cattcacaaa
    25921 ccgggtaaag tgggtatcgt ttcccgttcc ggtacactga cctatgaagc ggttaaacag
    25981 accacggatt acggtttcgg tcagtcgacc tgtgtcggta tcggcggtga cccgatccct
    26041 ggctctaact ttatcgacat tctcgaaatg ttcgaaaaag atccgcagac cgaagcgatc
    26101 gtgatgatcg gtgagatcgg cggtagcgct gaagaagaag cagctgcgta catcaaagag
    26161 cacgttacca agccagttgt gggttacatc gctggtgtga ctgcgccgaa aggcaaacgt
    26221 atgggccacg cgggtgccat cattgccggt gggaaaggga ctgcggatga gaaattcgct
    26281 gctctggaag ccgcaggcgt gaaaaccgtt cgcagcctgg cggatatcgg tgaagcactg
    26341 aaaactgttc tgaaataaat atctgtaata agaaatagcc ctcgccgctt ccctctacag
    26401 gaatggcgaa gggctgtcgg tttcgacatg gttggccatc gtatgatggc cttttttatg
    26461 ccttaaacgg ttaaccacgg tcgaaatctg actgcaggct tgactgcatg accacagcca
    26521 ctgtggattg ccgcaaccga ccgcaagcca cgaataagaa cgacggcgaa tttcgaatcg
    26581 ttacctgatg cactggcttc ccagcgtttg accgtagcgt taagtaaatc aacaaaagcg
    26641 atgttcaccg cttttcgtca agtcgtgcca tttgtcagcc aggatcgggt ctttaccccg
    26701 gatattgcag catcgcaaaa
//
LOCUS       DAZFFG010000068        26138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFFG010000068 DAZFFG010000000
VERSION     DAZFFG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.068"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..81
                     /locus_tag="ACHJ7J_003035"
     CDS             <1..81
                     /locus_tag="ACHJ7J_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001236.1"
                     /translation="MDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            264..458
                     /gene="bfd"
                     /locus_tag="ACHJ7J_003036"
     CDS             264..458
                     /gene="bfd"
                     /locus_tag="ACHJ7J_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO5001237.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            531..1007
                     /gene="bfr"
                     /locus_tag="ACHJ7J_003037"
     CDS             531..1007
                     /gene="bfr"
                     /locus_tag="ACHJ7J_003037"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO5001238.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGVPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1004..1471)
                     /locus_tag="ACHJ7J_003038"
     CDS             complement(1004..1471)
                     /locus_tag="ACHJ7J_003038"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO5001239.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1850..2161
                     /gene="rpsJ"
                     /locus_tag="ACHJ7J_003039"
     CDS             1850..2161
                     /gene="rpsJ"
                     /locus_tag="ACHJ7J_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO5001240.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2194..2823
                     /gene="rplC"
                     /locus_tag="ACHJ7J_003040"
     CDS             2194..2823
                     /gene="rplC"
                     /locus_tag="ACHJ7J_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO5001241.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2834..3439
                     /gene="rplD"
                     /locus_tag="ACHJ7J_003041"
     CDS             2834..3439
                     /gene="rplD"
                     /locus_tag="ACHJ7J_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO5001242.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALIHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3436..3738
                     /gene="rplW"
                     /locus_tag="ACHJ7J_003042"
     CDS             3436..3738
                     /gene="rplW"
                     /locus_tag="ACHJ7J_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO5001243.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3756..4577
                     /gene="rplB"
                     /locus_tag="ACHJ7J_003043"
     CDS             3756..4577
                     /gene="rplB"
                     /locus_tag="ACHJ7J_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO5001244.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4594..4872
                     /gene="rpsS"
                     /locus_tag="ACHJ7J_003044"
     CDS             4594..4872
                     /gene="rpsS"
                     /locus_tag="ACHJ7J_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO5001245.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4887..5219
                     /gene="rplV"
                     /locus_tag="ACHJ7J_003045"
     CDS             4887..5219
                     /gene="rplV"
                     /locus_tag="ACHJ7J_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO5001246.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5237..5938
                     /gene="rpsC"
                     /locus_tag="ACHJ7J_003046"
     CDS             5237..5938
                     /gene="rpsC"
                     /locus_tag="ACHJ7J_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO5001247.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5951..6361
                     /gene="rplP"
                     /locus_tag="ACHJ7J_003047"
     CDS             5951..6361
                     /gene="rplP"
                     /locus_tag="ACHJ7J_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO5001248.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6361..6552
                     /gene="rpmC"
                     /locus_tag="ACHJ7J_003048"
     CDS             6361..6552
                     /gene="rpmC"
                     /locus_tag="ACHJ7J_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO5001249.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6552..6806
                     /gene="rpsQ"
                     /locus_tag="ACHJ7J_003049"
     CDS             6552..6806
                     /gene="rpsQ"
                     /locus_tag="ACHJ7J_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO5001250.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6971..7342
                     /gene="rplN"
                     /locus_tag="ACHJ7J_003050"
     CDS             6971..7342
                     /gene="rplN"
                     /locus_tag="ACHJ7J_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO5001251.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7353..7667
                     /gene="rplX"
                     /locus_tag="ACHJ7J_003051"
     CDS             7353..7667
                     /gene="rplX"
                     /locus_tag="ACHJ7J_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO5001252.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7682..8221
                     /gene="rplE"
                     /locus_tag="ACHJ7J_003052"
     CDS             7682..8221
                     /gene="rplE"
                     /locus_tag="ACHJ7J_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO5001253.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8236..8541
                     /gene="rpsN"
                     /locus_tag="ACHJ7J_003053"
     CDS             8236..8541
                     /gene="rpsN"
                     /locus_tag="ACHJ7J_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO5001254.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8575..8967
                     /gene="rpsH"
                     /locus_tag="ACHJ7J_003054"
     CDS             8575..8967
                     /gene="rpsH"
                     /locus_tag="ACHJ7J_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO5001255.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8980..9513
                     /gene="rplF"
                     /locus_tag="ACHJ7J_003055"
     CDS             8980..9513
                     /gene="rplF"
                     /locus_tag="ACHJ7J_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO5001256.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9523..9876
                     /gene="rplR"
                     /locus_tag="ACHJ7J_003056"
     CDS             9523..9876
                     /gene="rplR"
                     /locus_tag="ACHJ7J_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO5001257.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9891..10394
                     /gene="rpsE"
                     /locus_tag="ACHJ7J_003057"
     CDS             9891..10394
                     /gene="rpsE"
                     /locus_tag="ACHJ7J_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO5001258.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10398..10577
                     /gene="rpmD"
                     /locus_tag="ACHJ7J_003058"
     CDS             10398..10577
                     /gene="rpmD"
                     /locus_tag="ACHJ7J_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO5001259.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10581..11015
                     /gene="rplO"
                     /locus_tag="ACHJ7J_003059"
     CDS             10581..11015
                     /gene="rplO"
                     /locus_tag="ACHJ7J_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO5001260.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11023..12354
                     /gene="secY"
                     /locus_tag="ACHJ7J_003060"
     CDS             11023..12354
                     /gene="secY"
                     /locus_tag="ACHJ7J_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO5001261.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12386..12502
                     /gene="rpmJ"
                     /locus_tag="ACHJ7J_003061"
     CDS             12386..12502
                     /gene="rpmJ"
                     /locus_tag="ACHJ7J_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO5001262.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12649..13005
                     /gene="rpsM"
                     /locus_tag="ACHJ7J_003062"
     CDS             12649..13005
                     /gene="rpsM"
                     /locus_tag="ACHJ7J_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO5001263.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13022..13411
                     /gene="rpsK"
                     /locus_tag="ACHJ7J_003063"
     CDS             13022..13411
                     /gene="rpsK"
                     /locus_tag="ACHJ7J_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO5001264.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13445..14065
                     /gene="rpsD"
                     /locus_tag="ACHJ7J_003064"
     CDS             13445..14065
                     /gene="rpsD"
                     /locus_tag="ACHJ7J_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO5001265.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14091..15080
                     /gene="rpoA"
                     /locus_tag="ACHJ7J_003065"
     CDS             14091..15080
                     /gene="rpoA"
                     /locus_tag="ACHJ7J_003065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO5001266.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15121..15504
                     /gene="rplQ"
                     /locus_tag="ACHJ7J_003066"
     CDS             15121..15504
                     /gene="rplQ"
                     /locus_tag="ACHJ7J_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO5001267.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15611..15979
                     /gene="yhdN"
                     /locus_tag="ACHJ7J_003067"
     CDS             15611..15979
                     /gene="yhdN"
                     /locus_tag="ACHJ7J_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO5001268.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15990..16415
                     /gene="zntR"
                     /locus_tag="ACHJ7J_003068"
     CDS             15990..16415
                     /gene="zntR"
                     /locus_tag="ACHJ7J_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO5001269.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16471..16689
                     /gene="arfA"
                     /locus_tag="ACHJ7J_003069"
     CDS             16471..16689
                     /gene="arfA"
                     /locus_tag="ACHJ7J_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO5001270.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16686..17096)
                     /gene="mscL"
                     /locus_tag="ACHJ7J_003070"
     CDS             complement(16686..17096)
                     /gene="mscL"
                     /locus_tag="ACHJ7J_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO5001271.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFSVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17226..18602)
                     /gene="trkA"
                     /locus_tag="ACHJ7J_003071"
     CDS             complement(17226..18602)
                     /gene="trkA"
                     /locus_tag="ACHJ7J_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO5001272.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18624..19913)
                     /gene="rsmB"
                     /locus_tag="ACHJ7J_003072"
     CDS             complement(18624..19913)
                     /gene="rsmB"
                     /locus_tag="ACHJ7J_003072"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO5001273.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDNWLALLDEAGMKGFPHTDYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19959..20906)
                     /gene="fmt"
                     /locus_tag="ACHJ7J_003073"
     CDS             complement(19959..20906)
                     /gene="fmt"
                     /locus_tag="ACHJ7J_003073"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO5001274.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20921..21445)
                     /gene="def"
                     /locus_tag="ACHJ7J_003074"
     CDS             complement(20921..21445)
                     /gene="def"
                     /locus_tag="ACHJ7J_003074"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO5001275.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARALKARA"
     gene            21575..22699
                     /gene="dprA"
                     /locus_tag="ACHJ7J_003075"
     CDS             21575..22699
                     /gene="dprA"
                     /locus_tag="ACHJ7J_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO5001276.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22671..23144
                     /gene="smg"
                     /locus_tag="ACHJ7J_003076"
     CDS             22671..23144
                     /gene="smg"
                     /locus_tag="ACHJ7J_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO5001277.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23173..23715
                     /gene="yrdD"
                     /locus_tag="ACHJ7J_003077"
     CDS             23173..23715
                     /gene="yrdD"
                     /locus_tag="ACHJ7J_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO5001278.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23720..24292
                     /gene="tsaC"
                     /locus_tag="ACHJ7J_003078"
     CDS             23720..24292
                     /gene="tsaC"
                     /locus_tag="ACHJ7J_003078"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO5001279.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24297..25115
                     /gene="aroE"
                     /locus_tag="ACHJ7J_003079"
     CDS             24297..25115
                     /gene="aroE"
                     /locus_tag="ACHJ7J_003079"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO5001280.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25112..25369
                     /gene="yrdB"
                     /locus_tag="ACHJ7J_003080"
     CDS             25112..25369
                     /gene="yrdB"
                     /locus_tag="ACHJ7J_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO5001281.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25345..25899)
                     /gene="yrdA"
                     /locus_tag="ACHJ7J_003081"
     CDS             complement(25345..25899)
                     /gene="yrdA"
                     /locus_tag="ACHJ7J_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO5001282.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6701 a         6193 c         6784 g         6460 t
ORIGIN      
        1 atggacgacg gtctgcgttt cgcaatccgt gaaggcggcc gtaccgttgg cgcgggcgtt
       61 gtagctaaag ttctgggcta attacacgtt aattagtttt gaattgaaaa gggcgcttcg
      121 gcgccctttt tgctgcccgt aatttgagct cgaaatattt agtcgtaatt ttgctcgctg
      181 aactatggcg caaataaaac tgtgctattg taatcataac tattctcatt tacactttgc
      241 gcagaatttg aacgggagca atcatgtacg tttgtttgtg taatggtgta agtgacaaaa
      301 aaattcgcca ggcagtccgt cagtttcatc cgcaatcttt ccaacaattg cgtaagttta
      361 ttcctgtggg aaatcaatgt ggtaagtgca ttcgcgccgc gcgcgaagtg atgcaggacg
      421 agttgatgca gatgccagaa tttaaagaga ttgcctgaag cccacgctta ttttttgaca
      481 tcccgctagc cccatctacg ctctaatagt ggaagcggag ggactataaa atgaaaggtg
      541 atgttaaaat cataaattat ctcaataaac tattgggaaa tgagcttgtc gcaatcaatc
      601 agtattttct ccatgcccgg atgtttaaaa actggggact gatgcgtctt aatgacgtcg
      661 aataccatga atccatcgat gagatgaaac acgctgataa atatatcgag cgtattttgt
      721 ttttagaagg agtccccaac ttacaggatt taggcaaact ggggattggt gaagatgtcg
      781 aagagatgct gcaatccgac ctgcgattag agctggaagg tgcgaaggat ctgcgtgagg
      841 ctatcgcata tgccgacagc gttcatgact atgttagccg cgatatgatg atcgagatcc
      901 ttgccgatga agaaggtcat atcgactggc tcgaaactga actggatctc atcggtaaaa
      961 tcggcctaca aaattacctg caatcacaaa ttaaagttaa agattaacgg cctatcagca
     1021 aactatccca gcctataacg aaacccgcag ccgccagaaa tggcccaaaa ggcagcgggt
     1081 tttttaatga ttcttttcct ctcatgagca gaccaataac tacggctccg caagcgaatg
     1141 aggcagcaag gaaaaccagt cgtggtaaga aggcccatga atgccaggcg ccaagggcgg
     1201 cgagaaactt cacatcacca tagcctaatc cttctttatg gcgcagtatg cgataccccc
     1261 agtaaatgac agcgaatgtg ccgtaaccaa taattgcccc ccataacgca tcggctaaac
     1321 catcaggatg acaaacttga tagaacaaaa gacctgacca gagtaacggg cacgtaaatc
     1381 tgtcaggtaa caagccatgc tttgcatccc agaaaaaaag tagggctgat aaacaagcgt
     1441 acaaaattag aaagggaagg gtagtgtcca taaaacatcc tctgtaattg gagtatcacc
     1501 atcgaataac tccggatgct cagcaacaga gagagcgctg atttgttaac ctgattccgg
     1561 tgaaaaacgc tatcttcaca accttacaaa tttcgtggaa tgcagctcgc aatctgcgcg
     1621 ttgcgattaa tttctgcccg atgcttgcgt caggctcaga tttgcgtata atgcgcgggc
     1681 ttgtcgtagt tgacagcagg ttcaatctga acctcagtta gccgaatttg gctacctaac
     1741 aatgctccca atcggggagc tacgtaagaa cggttacact ctcccatcaa tcgtaatggg
     1801 tctgaggagt aatcattttc gtttataaaa taattggagc tctggtctca tgcagaacca
     1861 aagaatccgt atccgcctga aagcgtttga tcatcgtctg atcgatcaag caaccgcgga
     1921 aatcgtcgag actgccaagc gcactggtgc gcaggtccgt ggtccgatcc cgctgccgac
     1981 acgcaaagag cgcttcactg ttctgatctc cccgcacgtc aacaaagacg cgcgcgatca
     2041 gtacgaaatc cgtactcact tgcgtctggt tgacatcgtt gagccaaccg agaaaaccgt
     2101 tgatgctctg atgcgtctgg atctggctgc cggtgtagac gtgcagatca gcctgggtta
     2161 atcaggtcat tgagcgattg agaggttgaa acaatgattg gtttagtcgg taaaaaagtg
     2221 ggtatgaccc gtatcttcac agaagacggc gtttctatcc cagtaaccgt aatcgaagtt
     2281 gaagcaaacc gcgttactca ggttaaagac ctggctaacg atggctaccg tgctattcag
     2341 gtgaccaccg gtgctaaaaa agctaaccgt gtgaccaagc ctgaagctgg ccacttcgct
     2401 aaagctggcg tagaagctgg ccgtggtctg tgggaattcc gcctggctga aggcgaagag
     2461 ttcactgtag gtcagagcat tagcgttgaa ctgtttgctg acgttaaaaa agttgacgta
     2521 actggcacct ctaaaggtaa aggtttcgca ggtaccgtta agcgctggaa cttccgtacc
     2581 caggacgcta ctcacggtaa ctccttgtct caccgcgttc cgggttctat cggtcagaac
     2641 cagactccgg gcaaagtgtt caaaggcaag aaaatggcag gtcagatggg taacgaacgt
     2701 gtaaccgttc agagccttga cgtagtacgc gttgacgctg agcgcaacct gctgctggtt
     2761 aaaggtgctg tcccgggtgc aaccggtagc gacctgatcg ttaaaccagc tgtgaaggcg
     2821 taaggagata gcaatggaat tagtattgaa agacgcgcag agcgcgctga ctgtttccga
     2881 aactaccttc ggtcgtgatt tcaacgaagc gctgattcac caggttgttg ttgcttatgc
     2941 agctggtgct cgtcagggta ctcgtgctca gaagactcgt gctgaagtaa ctggttccgg
     3001 taaaaaaccg tggcgccaga aaggcaccgg ccgtgcgcgt tctggttcta tcaagagccc
     3061 gatctggcgt tctggtggcg tgacctttgc tgctcgtccg caggaccaca gtcaaaaagt
     3121 taacaagaag atgtaccgcg gcgcgctgaa aagcatcctg tccgaactgg tacgtcagga
     3181 tcgtctgatc gttgtcgaga agttctctgt agaagcgccg aaaactaagc tgctggcaca
     3241 gaaactgaaa gacatggctc tggaagatgt gctgatcatc accggtgagc tggacgaaaa
     3301 cctgttcctg gctgcgcgca acctgcacaa ggttgacgta cgcgatgcaa ctggtatcga
     3361 cccggttagc ctgatcgcct tcgacaaagt cgtaatgact gctgatgctg ttaagcaagt
     3421 tgaggagatg ctggcatgat tcgtgaagaa cgtctgctga aggtgctgcg tgcaccgcac
     3481 gtttctgaaa aagcgtctac tgcgatggaa aaatccaaca ccatcgtact caaagttgct
     3541 aaagacgcga ccaaagcaga aatcaaagct gctgtgcaga aactgtttga agtcgaagtc
     3601 gaagtcgtta acaccctggt agttaaaggg aaagttaaac gtcacggaca gcgtatcggt
     3661 cgtcgtagcg actggaaaaa agcttacgtc accctgaaag aaggccagaa tctggacttc
     3721 gttggcggcg ctgagtaagt cggaggagta atacaatggc agttgttaaa tgtaaaccga
     3781 catctccggg tcgtcgccac gtagttaaag tggttaaccc tgagctgcac aagggcaaac
     3841 cttttgctcc gttgctggaa aaaaacagca aatccggtgg tcgtaacaac aatggccgta
     3901 tcaccactcg tcatatcggt ggtggccaca agcaggctta ccgtattgtt gacttcaaac
     3961 gcaacaaaga cggtatcccg gcagttgttg aacgtcttga gtacgatccg aaccgttccg
     4021 cgaacatcgc gctggttctg tacaaagacg gtgaacgccg ttacatcctg gcccctaaag
     4081 gcctgaaagc tggcgaccag attcagtctg gcgttgatgc tgcaatcaaa ccaggtaaca
     4141 ccctgccgat gcgcaacatc ccggttggtt ctactgttca taacgtagaa atgaaaccag
     4201 gtaaaggcgg tcagctggca cgttccgctg gtacttacgt tcagatcgtt gctcgtgatg
     4261 gtgcttatgt caccctgcgt ctgcgttctg gtgaaatgcg taaagtagaa gcagactgcc
     4321 gtgcaactct gggcgaagtt ggcaatgctg agcatatgct gcgcgttctg ggtaaagcag
     4381 gtgctgcacg ctggcgtggt gttcgtccga ccgttcgcgg taccgcgatg aacccggtag
     4441 accacccaca tggtggtggt gaaggtcgta actttggtaa gcacccggta actccgtggg
     4501 gcgttcagac caaaggtaag aagacccgca gcaacaagcg tactgataaa ttcatcgtac
     4561 gtcgccgtag caaataattt tagaggataa gccatgccac gttctctcaa gaaaggtcct
     4621 tttattgacc tgcacttgct gaagaaggta gagaaagcgg tggaaagcgg agacaagaag
     4681 cccctgcgca cttggtcccg tcgttcaacg atctttccta acatgatcgg tttgaccatc
     4741 gctgtccata atggtcgtca gcacgttccg gtatttgtaa ccgacgaaat ggtcggtcac
     4801 aaactgggtg aattcgcacc gactcgtact tatcgcggcc acgctgctga taaaaaagcg
     4861 aagaagaaat aaggtaggag gaagagatgg aaactatcgc taaacatcgc catgctcgtt
     4921 cttctgctca gaaggttcgc cttgttgctg acctgattcg cggtaagaaa gtgtcgcagg
     4981 ctctggatat tttgacctac accaacaaga aagcggctgt actggtcaag aaagttctgg
     5041 aatctgccat tgctaacgct gaacacaacg atggcgctga cattgacgat ctgaaagtta
     5101 cgaaaatttt cgtagacgaa ggcccgagca tgaagcgcat tatgccgcgt gcaaaaggtc
     5161 gtgcagatcg catcctgaag cgcaccagcc acatcactgt ggttgtgtcc gatcgctgag
     5221 actctggaga ctagcaatgg gtcagaaagt acatcctaat ggtattcgcc tgggtattgt
     5281 aaaaccatgg aactctacct ggtttgcgaa caccaaagaa ttcgctgaca acctggacag
     5341 cgattttaaa gtacgtcagt acctgactaa ggaactggct aaagcgtccg tatctcgtat
     5401 cgttatcgag cgtccggcta agagcatccg tgtaaccatt cacactgctc gcccgggtat
     5461 cgttatcggt aaaaaaggtg aagacgtaga aaaactgcgt aaggtcgtag cggacatcgc
     5521 tggcgttcct gcacagatca acatcgccga agttcgtaag cctgaactgg acgcaaaact
     5581 ggttgctgac agcatcactt ctcagctgga acgtcgcgtt atgttccgtc gtgctatgaa
     5641 gcgtgctgta cagaacgcaa tgcgtctggg cgctaaaggt attaaagttg aagttagcgg
     5701 ccgtctgggc ggcgcggaaa tcgcacgtac cgaatggtac cgcgaaggtc gcgtaccgct
     5761 gcacactctg cgtgctgaca tcgactacaa cacctctgaa gcgcacacca cttacggtgt
     5821 aatcggcgtt aaagtgtgga tcttcaaagg cgagatcctg ggtggtatgg ctgctgttga
     5881 acaaccggaa aaaccggctg ctcagcctaa aaagcagcag cgtaaaggcc gtaaataagg
     5941 agcgtcgctg atgttacaac caaagcgtac aaaattccgt aaaatgcaca aaggccgtaa
     6001 ccgcggtctg gcgcagggta cggatgttag cttcggcagc ttcggtctga aagctgttgg
     6061 ccgtggtcgt ctgactgccc gtcagatcga agcagcacgt cgtgctatga cccgtgcagt
     6121 taagcgtcaa ggtaagatct ggatccgtgt gttcccggac aaaccgatca ctgaaaagcc
     6181 gctggcagtg cgtatgggta aaggtaaagg taacgtggag tattgggttg ccttgattca
     6241 gccgggtaaa gtcctgtatg aaatggacgg tgttccggaa gagctggccc gtgaagcatt
     6301 caagctggca gcagcgaaac tgccgattaa aaccaccttt gtaactaaga cggtgatgta
     6361 atgaaagcaa aagagctgcg tgagaagagc gttgaagagc tgaacaccga gctgctgaac
     6421 ctgctgcgtg agcagttcaa cctgcgtatg caggctgcaa gtggccagct gcaacagtct
     6481 cacctgttga agcaagtgcg tcgcgatgtc gcacgcgtta agactttact gaacgagaag
     6541 gcgggtgcgt aatgaccgat aaaatccgta ctctgcaagg tcgcgttgtt agcgacaaaa
     6601 tggagaaatc cattgttgtt gctatcgaac gttttgtgaa acacccgatc tacggtaaat
     6661 tcatcaagcg tacgaccaaa ctgcacgtac atgacgagaa caacgaatgc ggtatcggtg
     6721 acgtggttga aatccgcgaa tgccgtccgc tgtccaagac taaatcctgg acgctggttc
     6781 gcgttgtaga gaaagcggtt ctgtaataca gtacactctc tcgatacgaa taaacggctc
     6841 agaaatgagc cgtttatttt ttctacccat atccttgaag cggtgttata atgccgcgcc
     6901 ctcgatatgg ggatttttaa cgacctgatt ttcgggtctc agtagtagtt gacattagcg
     6961 gagcactaaa atgatccaag aacagactat gctgaacgtc gccgacaact ccggtgcacg
     7021 tcgcgtaatg tgtatcaagg ttctgggtgg ctcgcaccgt cgctacgcag gcgtaggcga
     7081 catcatcaag atcaccatca aagaagcaat tccgcgtggt aaggtcaaaa aaggtgatgt
     7141 gctgaaggcg gtagtggtgc gcaccaagaa gggtgttcgt cgcccggacg gttctgtcat
     7201 tcgcttcgat ggtaatgctt gtgttcttct gaacaacaac agcgagcagc ctatcggtac
     7261 gcgtattttt gggccggtaa ctcgtgagct tcgtagtgag aagttcatga aaattatctc
     7321 tctggcacca gaagtactct aaggagcgaa tcatggcagc gaaaatccgt cgtgatgacg
     7381 aagttatcgt gttaaccggt aaagataaag gtaaacgcgg taaagttaag aatgtcctgt
     7441 cttccggcaa ggtcattgtt gaaggtatca acctggttaa gaaacatcag aagccggttc
     7501 cggccctgaa ccaaccgggt ggcatcgttg aaaaagaagc cgctattcag gtttccaacg
     7561 tagcaatctt caatgcggca accggcaagg ctgaccgtgt aggctttaga ttcgaagacg
     7621 gcaaaaaagt ccgtttcttc aagtctaaca gcgaaactat caagtaattt ggagtagtac
     7681 gatggcgaaa ctgcatgatt actacaaaga cgaagtagtt aaaaaactca tgactgagtt
     7741 taactacaat tctgtcatgc aagtccctcg ggtcgagaag atcaccctga acatgggtgt
     7801 tggtgaagcg atcgctgaca aaaaactgct ggataacgca gcagcagacc tggcagcaat
     7861 ctccggtcaa aaaccgctga tcaccaaagc acgcaaatct gttgcaggct tcaaaatccg
     7921 tcagggctat ccgatcggct gtaaagtaac tctgcgtggc gaacgcatgt gggagttctt
     7981 tgagcgcctg atcactattg ctgtacctcg tatccgtgac ttccgtggcc tgtccgctaa
     8041 gtctttcgac ggtcgtggta actacagcat gggtgtccgt gagcagatca tcttcccaga
     8101 aatcgactac gataaagtcg accgcgttcg tggtttggat attaccatta ccactactgc
     8161 gaaatctgac gaagaaggcc gcgctctgct ggctgccttt gacttcccgt tccgcaagta
     8221 aggtagggtt actaaatggc taagcaatca atgaaagcac gcgaagtaaa acgcgtagct
     8281 ttagctgata aatacttcgc gaaacgcgct gaactgaaag cgatcatctc tgatgtgaac
     8341 gcttccgacg aagatcgttg gaacgctgtt ctcaagctgc agactctgcc gcgtgattcc
     8401 agcccgtctc gtcagcgtaa ccgctgccgt caaacaggtc gtccgcatgg tttcctgcgg
     8461 aagttcgggt tgagccgtat taaggtccgt gaagccgcta tgcgcggtga aatcccgggt
     8521 ctgaaaaagg ctagctggta attgtcacca attgaatcac gggagtaaag acagatgagc
     8581 atgcaagatc cgatcgcgga tatgctgacc cgtatccgta acggtcaggc cgcgaacaaa
     8641 gctgcggtca ccatgccttc ctccaagctg aaagtggcaa tcgccaacgt gctgaaggaa
     8701 gaaggtttta ttgaagattt taaagttgaa ggcgacacca agcctgaact ggaacttact
     8761 ctgaagtatt tccagggcaa agctgttgta gaaagcattc agcgtgtcag ccgcccaggt
     8821 ctgcgcatct ataaacgtaa agatgagctg ccgaaagtta tggcgggtct gggtatcgca
     8881 gttgtttcta cctctaaagg tgttatgact gatcgtgcag cgcgccaggc tggtcttggt
     8941 ggcgaaatta tctgctacgt agcctaatcg gaggaaaaaa tgtctcgtgt tgctaaagca
     9001 ccggtcgttg ttcctgccgg cgttgacgta aaaatcaacg gtcaggttat tacgatcaaa
     9061 ggtaaaaacg gcgagctgac tcgtactctc aacgatgctg ttgaagttaa acatgcagat
     9121 aataccctga ccttcggtcc gcgtgatggt tacgcagacg gttgggcaca ggctggtacc
     9181 gcgcgtgccc tgctgaactc aatggttatc ggtgttaccg aaggcttcac taagaagctg
     9241 cagctggttg gtgtaggtta ccgtgcagcg gttaaaggca atgtgattaa cctgtctctg
     9301 ggtttctctc atcctgttga ccatcagctg cctgcgggta tcactgctga atgtccgact
     9361 cagactgaaa tcgtgctgaa aggcgctgat aagcaggtga tcggccaggt tgcagcggat
     9421 ctgcgcgcct accgtcgtcc tgagccttat aaaggcaagg gtgttcgtta cgccgacgaa
     9481 gtcgtgcgta ccaaagaggc taagaagaag taaggtaaca ctatggataa gaaatctgct
     9541 cgtatccgtc gtgcgacccg cgcacgccgc aagctccagg agctgggcgc aactcgcctg
     9601 gtggtacatc gtaccccgcg tcacatttac gcacaggtaa ttgcaccgaa cggttctgaa
     9661 gttctggtag ctgcttctac tgtagaaaaa gctatcgctg aacaactgaa gtacaccggt
     9721 aacaaagacg cggctgcagc tgtgggtaaa gctgtcgctg aacgcgctct ggaaaaaggc
     9781 atcaaagatg tatcctttga ccgttccggg ttccaatatc atggtcgtgt ccaggcactg
     9841 gcagatgctg cccgtgaagc tggccttcag ttctaaggta gaggtgtaag atggctcaca
     9901 tcgaaaaaca agctggcgaa ctgcaggaaa agctgatcgc ggtaaaccgc gtatctaaaa
     9961 ccgttaaagg tggtcgtatt ttctccttca cagctctgac tgtagttggc gatggtaacg
    10021 gtcgcgttgg ttttggttac ggtaaagcgc gtgaagttcc agcagcgatc cagaaagcga
    10081 tggaaaaagc ccgtcgcaat atgattaacg tcgcgctgaa taacggcact ctgcaacacc
    10141 ctgttaaagg tgttcacacg ggttctcgcg tattcatgca gccggcttcc gaaggtaccg
    10201 gtatcatcgc cggtggtgca atgcgcgccg ttctggaagt cgctggggtt cataacgttc
    10261 tggctaaagc atatggttcc accaacccga tcaacgtggt tcgtgcaact attgatggcc
    10321 tggaaaatat gaattctcca gaaatggtcg ctgccaagcg tggtaaatcc gttgaagaaa
    10381 ttctggggaa ataaaccatg gcaaagacta ttaaaattac tcaaacccgc agtgcaatcg
    10441 gtcgtctgcc gaaacacaag gcaacgctgc ttggcctggg tctgcgtcgt attggtcaca
    10501 ccgtagagcg cgaggatact cctgctattc gcggtatgat caacgcggtt tccttcatgg
    10561 ttaaagttga ggagtaagag atgcgtttaa atactctgtc tccggccgaa ggctccaaaa
    10621 aggcgggtaa acgcctgggt cgtggtatcg gttctggcct cggtaaaacc ggtggtcgtg
    10681 gtcacaaagg tcagaagtct cgttctggcg gtggcgtacg tcgcggtttc gagggtggtc
    10741 agatgcctct gtaccgtcgt ctgccgaaat tcggcttcac ttctcgtaaa gcagcgatta
    10801 cagccgaagt tcgtctgtct gacctggcta aagtagaagg cggtgtagta gacctgaaca
    10861 cgctgaaagc ggctaacatt atcggtatcc agatcgagtt cgcgaaagtg atcctggctg
    10921 gcgaagtgac gactccggta actgttcgtg gcctgcgtgt tactaaaggc gctcgtgctg
    10981 ctatcgaagc tgctggcggt aaaatcgagg aataagtagc agatggctaa acaaccggga
    11041 ttagattttc aaagtgccaa aggtggctta ggcgagctga aacgcagact gctgtttgtt
    11101 atcggtgcgc tgattgtgtt ccgtattggc tcttttattc cgatccctgg tattgatgcc
    11161 gctgtacttg ccaaactgct tgagcaacag cgaggcacca tcattgagat gtttaacatg
    11221 ttctctggtg gtgctctcag ccgtgcttct atctttgctc tggggatcat gccgtatatt
    11281 tcggcgtcga tcattatcca gctgctgacg gtggttcacc caacgttggc agaaattaag
    11341 aaagaagggg agtctggtcg tcgtaagatc agccagtaca cccgctacgg tactctggtg
    11401 ctggcaatat tccagtcgat cggtattgct accggtctgc cgaatatgcc tggtatgcaa
    11461 ggcctggtga ttaacccggg ctttgcattc tacttcaccg ctgttgtaag tctggtcaca
    11521 ggaacgatgt tcctgatgtg gttgggcgaa cagatcactg aacgaggtat cggcaacggt
    11581 atttcaatca ttatcttcgc cggtattgtc gcgggactcc cgccagccat tgcccatact
    11641 atcgagcaag cgcgtcaagg cgacctgcac ttcctcgtgt tgctgttggt tgcagtatta
    11701 gtatttgcag tgacgttctt tgttgtattt gttgagcgtg gtcaacgccg cattgtggta
    11761 aactacgcga aacgtcagca aggtcgtcgt gtctatgctg cacagagcac acatttaccg
    11821 ctgaaagtga atatggcggg ggtaatcccg gcaatcttcg cttccagtat tattctgttc
    11881 ccggcgacca tcgcgtcatg gttcgggggc ggtactggtt ggaactggct gacaacaatt
    11941 tcgctgtatt tgcagcctgg gcaaccgctt tatgtgttac tctatgcgtc tgcaatcatc
    12001 ttcttctgtt tcttctacac ggcgttggtt ttcaacccgc gtgaaacagc agataacctg
    12061 aagaagtccg gtgcatttgt accaggaatt cgtccgggag agcaaacggc gaagtatatc
    12121 gataaagtaa tgacccgcct gaccctggtt ggtgcgctgt atattacctt tatctgcctg
    12181 atcccggagt tcatgcgtga tgcaatgaaa gtaccgttct acttcggtgg gacctcactg
    12241 cttatcgttg ttgtcgtgat tatggacttt atggctcaag tgcaaactct gatgatgtcc
    12301 agtcagtatg agtctgcatt gaagaaggcg aacctgaaag gctacggccg ataattggtc
    12361 gcccgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
    12421 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
    12481 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
    12541 ggctagatta gccagccaat cttttgtatg tctgtgcgtt tccatttgag tatcctgaaa
    12601 acgggctttt cagcatggaa cgtacatatt aaatagtagg agtgcatagt ggcccgtata
    12661 gcaggcatta acattcctga tcataagcat gccgtaatcg cattaacttc gatttatggc
    12721 gtcggcaaga cccgttctaa agccatcctg gctgcagcgg gtatcgctga agatgttaag
    12781 atcagtgagc tgtctgaagg acaaatcgac acgctgcgtg acgaagttgc caaatttgtc
    12841 gttgaaggtg atctgcgccg tgaaatcagc atgagcatca agcgcctgat ggatcttggt
    12901 tgctatcgcg gtttgcgtca tcgtcgtggt ctcccggttc gcggtcagcg taccaagacc
    12961 aacgcacgta cccgtaaggg tccgcgcaaa ccgatcaaga aataatcggg gtgattgaat
    13021 aatggcaaag gcaccaattc gtgcacgtaa acgtgtaaga aaacaagtct ctgacggcgt
    13081 ggctcatatc catgcttctt tcaacaacac catcgtgact atcactgatc gtcagggtaa
    13141 cgcgttgggt tgggcaacag ccggtggttc cggtttccgt ggttctcgca aatccactcc
    13201 gtttgcagct caggttgcag cagagcgttg cgctgacgcc gtgaaagaat acggcatcaa
    13261 gaatctggaa gttatggtta aaggtccggg tccaggccgc gaatctacta ttcgtgctct
    13321 gaacgccgca ggtttccgca tcactaacat tactgatgtg actccgatcc ctcataacgg
    13381 ttgtcgtccg ccgaaaaaac gtcgcgtata acgcctcgtt ttccaggttt gttggagaaa
    13441 gaaaatggca agatatttgg gtcctaagct caagctgagc cgtcgtgagg gcaccgactt
    13501 attccttaag tctggcgttc gcgcgatcga taccaagtgt aaaattgaac aagctcctgg
    13561 ccagcacggt gcgcgtaaac cgcgtctgtc tgactatggt gtgcagttgc gtgaaaagca
    13621 aaaagttcgc cgtatctatg gtgtgctgga gcgtcagttc cgtaactact acaaagaagc
    13681 agcacgtctg aaaggcaaca ccggtgaaaa cctgttggct ctgctggaag gtcgtctgga
    13741 caacgttgta taccgtatgg gcttcggtgc cactcgtgca gaagcacgtc agctggttag
    13801 ccataaagca attatggtaa acggtcgtgt tgttaacatc gcttcttatc aggttagtcc
    13861 gaatgacgtt gtaagcattc gtgagaaagc gaagaagcag tctcgcgtga aagccgctct
    13921 ggagctggct gagcagcgtg aaaagccaac ctggctggaa gttgatgctg gcaagatgga
    13981 aggtacgttt aagcgtaagc cggagcgttc tgatctgtct gcggacatta acgaacacct
    14041 gatcgtcgag ctttactcca agtaaagctt agtaccaaag agaggacaca atgcagggtt
    14101 ctgtgacaga gtttctaaaa ccgcgcctgg ttgatatcga gcaagtgagt tcgacgcacg
    14161 ccaaggtgac ccttgagcct ttagagcgtg gctttggcca tactctgggt aacgcactgc
    14221 gccgtattct gctctcatcg atgccgggtt gcgcggtgac cgaggttgag attgatggtg
    14281 tactacatga gtacagcacc aaagaaggcg ttcaggaaga tatcctggaa atcctgctca
    14341 acctgaaagg gctggcggtg agagttcagg gcaaagatga agttattctt accttgaata
    14401 aatctggcat tggccctgtg actgcagccg atatcaccca cgacggtgat gtcgaaatcg
    14461 tcaagccgca gcacgtgatc tgccacctga ccgatgagaa cgcgtctatt agcatgcgta
    14521 tcaaagttca gcgcggtcgt ggttatgtgc cggcttctac ccgaattcat tcggaagaag
    14581 atgagcgccc aatcggccgt ctgctggtcg acgcatgcta cagccctgtg gagcgtattg
    14641 cctacaatgt tgaagcagcg cgtgtagaac agcgtaccga cctggacaag ctggtcatcg
    14701 aaatggaaac caacggcaca atcgatcctg aagaggcgat tcgtcgtgcg gcaaccattc
    14761 tggctgaaca actggaagct ttcgttgact tacgtgatgt acgtcagcct gaagtgaaag
    14821 aagagaaacc agagttcgat ccgatcctgc tgcgccctgt tgacgatctg gaattgactg
    14881 tccgctctgc taactgcctt aaagcagaag ctatccacta tatcggtgat ctggtacaac
    14941 gtaccgaggt tgagctcctt aaaacgccta accttggtaa aaaatctctt actgagatta
    15001 aagacgtgct ggcttcccgt ggactgtctc tgggcatgcg cctggaaaac tggccaccgg
    15061 caagcatcgc tgacgagtaa ccggatcaca ggttaaggtt ttactgagaa ggataaggtc
    15121 atgcgccatc gtaagagtgg tcgtcaactg aaccgcaaca gcagccatcg ccaggctatg
    15181 ttccgcaata tggcaggttc actggttcgt catgaaatca tcaagacgac tctgcctaaa
    15241 gcgaaagagc tgcgccgcgt agttgagccg ctgattactc ttgccaagac tgatagcgtt
    15301 gctaatcgtc gtctggcatt cgcccgtact cgtgataacg agatcgtggc aaaactgttt
    15361 aatgaactgg gcccgcgttt cgcgagccgt gccggtggtt acactcgtat tctgaagtgt
    15421 ggcttccgtg caggcgacaa cgcgccgatg gcttacatcg agctggttga tcgttcagag
    15481 aaagcagaag ctgctgcaga gtaatctgaa gcaacgtaaa aaaacccgcc ccggcgggtt
    15541 tttttatacc cgtagcatcc ccacttatct acaatagctg tactcttttt gttcatcccc
    15601 tggagtattt atgtggttac ttgaccagtg ggcagagcgc catatagcag aagcgcaagc
    15661 gaaaggtgag tttgataacc tggcaggtag cggcgaacca ttgatactgg atgatgattc
    15721 tcatgtgcca ccggaattac gtgcggggta tcgcttgctg aagaatgccg gttgcttacc
    15781 gccagaactt gagcaacgga gggaagcaat tcagcttctg gatattctca aaggtatccg
    15841 tcacgatgat ccgcaatatc aagaggttag ccgtcgattg tcattactgg aattgaagct
    15901 gcgacaagct ggactgagta ccgatttttt acgcggcgat tatgctgaca agttgttgaa
    15961 taaaatcaac gataactagt ggagtatgta tgtatcgcat tggtgagctg gcaaaaatgg
    16021 cggaagtaac acccgacacg attcgttatt acgaaaaaca gcagatgatg gagcatgaag
    16081 tgcgtactga aggtgggttt cgcctgtata ccgaaagcga tctccagcga ttgaaattta
    16141 tccgccatgc cagacaacta ggtttcagtc tagagtcgat ccgcgagttg ctgtcgatcc
    16201 gtatcgatcc tgaacaccat acctgtcagg agtcaaaagg cattgtgcag gaaagattac
    16261 aggaagtcga agcacggata gccgagttgc agagtatgca gcgttccttg caacgcctta
    16321 acgatgcctg ttgtgggacc gcccatagca gtgtttattg ctcgattctt gaagctcttg
    16381 aacaaggggc gagtggcgtt aagagtggct gttgattttt tgcactgaca ggattacact
    16441 cgcgccgtta aataaccaac tggagttttt atgagccgat atcagcatac taaagggcag
    16501 ataaaggata atgcgataga agcattacta catgatccct tattccgaca gcgagtagag
    16561 aaaaataaga aagggaaagg cagttacatg cgaaaaggaa aacatggcaa tcgggggaac
    16621 tgggaggcca gtggcaagaa agtaaatcac ttttttacca ctggccttct gctttcaggc
    16681 gtttgttaag agcggttatt ctgctctttc agcaaatcac gaatttctgt cagtaatact
    16741 tcttctttag ttggtgctgg tgcggctgct ggttcttctt ttttccgatt cagtttgttg
    16801 attagcttaa tcgccataaa gatggcaaag gccacaatca gaaaatcaaa gacgttttga
    16861 atgaagacac cgtaatgcat cacaacagca gggatatccc cctgcgcatc gcgtagcgtg
    16921 acagaaaact gtttaaagtc gatcccgcca attaataagc ccagaggtgg catgatgata
    16981 tcggcaacca gtgaagatac aatcttcccg aatgccgcac cgataatgac acccaccgcc
    17041 aaatccacca cgttcccgcg catcgcaaat tcgcgaaatt ctttaataat gctcatgtta
    17101 ttctccctat gaagccgaca accataagtc taacaaatgt taagccattt tcctgccagg
    17161 aatgaattta attaatcctt gataatcaaa gggaaataat aaggcgtcat tagacgcctt
    17221 attaattaca agaagaaagg gcttggctgg aagagtcttt cgacgtcggt aataaatttt
    17281 ttatctgtga ggaacataat tacgtgatcg ccttgctcaa tgcgcagatt gtcattggca
    17341 atcatcacgt cgtttccacg taccaccgct ccaataatcg ttcctggcgg tagcttgatt
    17401 tcgtcaatga ctctgccgac aacgcgtgag gtgctttcat caccgtgagc aacggcttca
    17461 atagcttctg ctacgccgcg gcgcaatgag gaaacaccaa caatatctgc ttttcgcaca
    17521 tggctaagca acgcagaaat agttgcttgt tgtggtgaaa tcgcaatatc gatcacgctc
    17581 ccctgaacca gatccacata agcgcgacgc tggatcaata ccatcacctt tttcgcaccc
    17641 atacgtttgg caagcatggc ggacatgata ttggcctcgt catcgttggt gacggcaata
    17701 aacagatcaa cttgatcgat atgttcttca gccagcagtt cttgatccga cgcatcacca
    17761 aaaaagacga tcgtattctg caacttttcc gccagttcgg cagcgcgctg ctgattacgt
    17821 tcgatgagtt taacgctgta atctttttcc agacgacgcg ccagccctgc accgatatta
    17881 ccgccaccaa ccagcatgat ccgcttatac ggtttttcca gtcgctgtaa ttcactcatc
    17941 accgcgcgga tatgctgtga agcggcaata aagaacactt catcaccagc ctcaacaatg
    18001 gtcgaacctt gcggacgaat ggggcgatcg tggcggaaaa ttgctgccac acgagtatcg
    18061 atatgtggca tatgttcgcg catggtcgaa agtgcattac caatcagcgg gccgccataa
    18121 taggctttaa ccacagccag gctgacttta ccctcagcga agtttaccac ctgcaatgcg
    18181 ccgggatact caatcagtcg gtaaatatta tcgataacca actgctctgg tgcgatcaga
    18241 tgatcaatcg gtacagcatc tgaatgaaat agcttatcgg catcgcgcac gtagtctggt
    18301 gagcggatac gagcgatgcg attaggggtg ttgaaaagtg agtaggctac ctggcaggca
    18361 accatattgg tttcatctga actggttaca gcaaccagca tatcggcgtc gtcggcacct
    18421 gcctcccgca atacgcgtgg atgagagcca tgcccctgca cgactcgcag gtcaaattta
    18481 tcctgcaagg tccgcagacg ctcaccgttg gtatcgacaa cagtgatatc gttgttctcg
    18541 ccaaccaggt tttccgccag tgtgccgcca acctggccgg cacccagaat gataattttc
    18601 atcagtcgcg acccgttatc tcatcacttt ttgattagct tagcgtaaaa gaagccgtcg
    18661 ccctcttcgg caccaggcag attttgttta cccggttgct ctggtgttcc tgtttcgcaa
    18721 agttcggcat cagcggtacg ttgcaaaaag gctttaatct gcaggctatt ctcttccggt
    18781 aacaccgaac aggtggcata gaccagagtt ccaccggatt ttaaatgcgg ccaaatggcg
    18841 tcgagaattt cagactgcaa ttgcgcgagt tccgggatat cgcgatcgcg acgtaaccat
    18901 ttaatatccg gatggcgacg aatcacaccg gttgctgaac aaggcgcatc taataaaatg
    18961 cgatcaaact gttgctcgcc acaccattgg gaagggtaac ggccatcacc ttgtttcacg
    19021 gttgccttca taccaaggcg ttttaaattg tcgtaaacgc gagagaggcg ctgttcgtca
    19081 atatcaaccg caacaacctg cgcttctggt gccacctcaa ggatatgcgt tgttttaccg
    19141 ccgggggcgg cacaaagatc cagaatgtgt tcaccgtttt gtggcgcaag ccaggtcatg
    19201 caaccttgtg ctgatgcatc ctgaacggta acccatccgt cttcaaaacc aggtagcgca
    19261 tgaacaggtg caggtgtttc cagccgtaca gcatcagggt aatccgtatg tgggaagcct
    19321 ttcattcctg cttcatccag caatgcaagc cagttgtcgc gggaatgatg cgtacgatta
    19381 atgcgcagcc acattggcgg acgctggtta ttggcttcga cgatggattg ccactgctct
    19441 ggatacgctt tttgcagacg cttcagcaac caggaaggat gcagataacg tgcatcactg
    19501 gcattaaact cggctaataa ctcttcttgc tgacgctgga actggcgtaa tacaccgttt
    19561 atcaaccctt taagttgcgg acgcttaatt gcgatagcgc cttcaaccgt ttcagccagc
    19621 gcagcatgag gtggaatgcg ggtataaagc agttgataca aaccaaccat aatcaggtaa
    19681 tgcacggtcc gctgtttgcc ggtcatcgga cgggccatta acttattaat cagccagtct
    19741 aactgcgaaa gcgtacgcag tacgccaaag cacaactctt gaagaagtgc tttgtcttta
    19801 tcggaaactt tttgctggag cggtggcaga atgttgctta atgattgccc ttgctcgacg
    19861 acttgttcaa cggcctgggc cgccatgcta cgtaaattac gttgtttttt cataagtata
    19921 aaaatgcccg gcaagaccgg gcttggaaga gtggactatc aggccagacg gttgcccgga
    19981 acaaaccatt cccgacgaga attcaggagg tcttgcgcgc tcatcgcttt cttacccgca
    20041 ggttgtaatg agagcaggtt caggattcca tcaccagtcg caacctgaat gccttgtttg
    20101 ttggcttcaa ggatcgttcc tggtgcagcg ttggttgccg tatcaatgac cgatgctttc
    20161 cagactttaa ccggctgtcc ttcaatttcc acccagctca ttggccatgg attgaaagcg
    20221 cgaatgcagc gttcaagctg tgctgccgaa agtgaccagt caatacgcgc ttcttcttta
    20281 ctcaacttct cggcgtaagt gacaagagtt tcgtcctgaa cttctggttt cgccgtgccg
    20341 tctgccaatt gtttcaacgt ggtgataagc ccttgtgggc caagctctgc cagcttgtcg
    20401 tacagcgtac cactggtatc ttctgcagta atcgggcagg agagcttata gagcatgtca
    20461 ccggtgtcta aaccaacatc catttgcata atggtcacac cagtttctgc atcacccgcc
    20521 catagtgagc gttggattgg tgcagcaccg cgccagcgtg gcagcagtga accatgaacg
    20581 ttgatacagc caagacgcgg catctccagc actgctttag gcagaattaa gccataggcg
    20641 acgacaacca taacatcagc ctgtagatcg gcgaccagtt gctggttttc ttgtggacgc
    20701 agggaaacag gttgaaaaac gggcagacct ttttcctcag ccagaacttt aaccgggctg
    20761 ggcatcagtt ttttaccgcg tcctgccggt cggtctggct gggtgaacac gccaacgacg
    20821 ttatgaccag aagacaacag cgcgtcgaga tgacgcgctg caaagtcagg tgtacccgca
    20881 aaaataatac gtagtgattc tgacacgtta gttcttatcc ttaagcccgg gctttcaaag
    20941 cccgggcttt cagacgatcc agtttttcaa ctttctgacg aatacgttgt tgtttcagcg
    21001 gtgacagata atccataaac agtttgccga ccaggtgatc catctcatgc tgaatacaga
    21061 tggctaacag accatctgct tccagttcaa atggtttacc gtcgcggtcc agggcgcgaa
    21121 ttttaacttt ctctgcgcgt ggcactaaag cacgttgttc agggatcgac aggcatcctt
    21181 cttcaatgcc tgtttcgccg cttttttcta aaagctccgg attgattaac accagccgtt
    21241 cgtcacggtt ttccgaaaca tcaataacaa tgatacgttg atggatatca acctgggttg
    21301 ccgccaggcc aataccttct tctgcgtaca tcgtctcgaa catatcatcg acgatacgct
    21361 gaatttctgc attcacttct tctaccggtt tagcaacttt gcgaagccgc tcgtccggaa
    21421 tatgtaacac ttgcaaaact gacataaatc tccagagatg tgttcaggag ttagaaagat
    21481 tatttcttct attctagaca aatccccctc tgattgacag catcactgac caatcgcaaa
    21541 gattgctaag gctgcttatg gcagggagat aaggatggtc gatacagaaa tttggctgcg
    21601 tttaatgagc atcagcagct tgtacggcga tgatatggtc cgtatcgctc actgggtggc
    21661 aaaacagtcg catattgatg cggttgtatt gcagcaaaca gggcttacat tgcggcaggc
    21721 acaacgcttt ctttcatttc cacgaaagag tatcgaaagc tcactttctt ggttggagca
    21781 acccaaccat catttaattc ctgcggacag cgaattttat cctcctcaac ttctggcgac
    21841 gacagattac cccggcgcac tgtttgttga aggagaactg cacgcgctgc attcatttca
    21901 gcttgccgta gtggggagtc gggcgcattc atggtatggc gagcgatggg gacgattatt
    21961 ttgcgaaact ctggcgacgc gtggagtgac aattacgagt ggactggcgc gtggaatcga
    22021 tggtgtggcg cataaagcgg ccttacaggt aaatggcgtc agcattgctg tattagggaa
    22081 tggacttaat accattcatc cgcgtcgcca tgcccgactg gctaccagtt tgcttgaaca
    22141 tggtggggct cttgtctcgg aatttcccct cgatgttcct ccccttgctt acaatttccc
    22201 acgaagaaat cgcattatca gtggtctaag taaaggtgta ctggtggtgg aagcggcttt
    22261 gcgcagtggt tcgctggtga cagcacgttg tgcgcttgag caggggcgcg aagtttttgc
    22321 cttgccgggt ccattaggga atccgggaag cgaagggcct cactggttaa taaaacaagg
    22381 tgcgattctt gtgacggaac cggaagaaat tctggaaaac ttgcaatttg gattgcactg
    22441 gttgccagac gcccctgaaa attcatttta ttcaccagat caggaagacg tggcattgcc
    22501 atttcctgag ctcctggcta acgtaggaga tgaggtaaca cctgttgacg tcgtcgctga
    22561 acgtgccggc caacctgtgc cagaggtagt tactcaacta ctcgaactgg agttagcagg
    22621 atggatcgca gctgtacccg gcggctatgt ccgattgagg agggcatgcc atgttcgacg
    22681 tactaatgta tttgtttgaa acctatattc acacagaagc tgagttgcgt gtggatcaag
    22741 acaaacttga acaggatctt accgacgcag gatttgatcg agaagatatc tacaatgccc
    22801 tgctttggct ggaaaagctt gcggattatc aggaaggact ggcagaaccg atgcaactgg
    22861 cctctgatcc actctccatg cgtatttata cccctgagga gtgtgaaaga ctggatgcca
    22921 gctgtcgtgg ttttctgctt ttccttgagc aaattcaggt gcttaacctt gaaacccgtg
    22981 aaatggtgat agagcgagtg ctcgcgctgg ataccgcaga gttcgacctg gaagacctga
    23041 aatgggtgat cctgatggtg ttgttcaata ttccgggctg cgaaaatgcg taccagcaaa
    23101 tggaagaatt actctttgaa gtgaatgaag gtatgctgca ttaatcattt ctttaattca
    23161 gcataagttg ttatggcgaa atcagcactg ttcacggtgc gtaataatga gtcctgccca
    23221 aagtgcgggg ctgaactggt tattcgatcc gggaaacacg gtccgtttct tggctgctca
    23281 cagtatccgg cgtgtgacta cgtccgtcct ctgaaatctt cagcggatgg acatattgtc
    23341 aaagttctgg aggggcaggt ttgccctgca tgtggcgcaa atctggtatt acgccaggga
    23401 cgctttggta tgtttattgg ttgcagtaac taccctgaat gcgaacatac cgaacttatc
    23461 gataaaccgg acgaaacagc aattacatgt ccccaatgcc ggacgggcca tctggtccag
    23521 cgccgctccc gttatggcaa aacatttcac tcctgtgatc gctacccgga gtgtcaattt
    23581 gccattaact tcaaacccat agctggagaa tgccctgagt gtcattatcc gctactcatc
    23641 gaaaagaaaa ccgcgcaggg tgtaaaacac ttttgtgcca gtaaacaatg tggaaagccg
    23701 gtttcggcgg aataataacg tgaataataa cctgcaagga gacgctatcg cagctgcgat
    23761 agatgttctc aatgaagaac gtgtcatcgc ctatccaacg gaagccgttt tcggtgttgg
    23821 gtgcgatcct gatagcgaaa cagcagtgat gcgactgttg gagttaaaac agcgtccggt
    23881 tgataagggg ctgattttaa tcgcagcaaa ttacgagcag cttaaaccct atattgatga
    23941 caccatgttg actgacgcgc agcgtgaaac cattttttcc cgctggccag gtccagtcac
    24001 ctttgtcttt cccgcgcctg cgacaacacc gcgctggttg acgggccgct ttgattcgct
    24061 tgctgtacga gtcaccgacc atccgttggt ggttgctttg tgccaagctt atggtaaacc
    24121 gctggtttct accagtgcca acttgagtgg attgccacct tgtcgaacag tagacgaagt
    24181 tcgcgcacaa tttggcgcgg cgttcccggt tgtgcctggt gaaacggggg ggcgtttaaa
    24241 tccttcagaa atccgcgatg ccctgacggg tgaactgttt cgacaggggt aacataatgg
    24301 aaacctatgc tgtttttggt aatccgatag cccacagcaa atcgccattc attcatcagc
    24361 aatttgctca gcaactgaat attgaacatc cctatgggcg cgtgttggca cccatcaatg
    24421 atttcatcaa cacactgaac gctttctttc gtgctggtgg taaaggtgcg aatgtgacgg
    24481 tgccttttaa agaagaggct tttgccagag cggatgagct tactgaacgg gcagcgttgg
    24541 ctggagcagt taataccctc aagcgtttag aagatggacg cctgctgggg gacaataccg
    24601 atggtatagg cttgttaagc gatctggaac gtctgtcttt tatccgtcct ggtttacgta
    24661 ttctgctcat cggcgctggt ggagcatctc gcggagtact actgccactt ctttccctgg
    24721 actgtgcggt gacaatcacc aatcgaacgg tatcccgcgc ggaagagttg gctaaattgt
    24781 ttgcgcacac tggcagtatt caggcgttgg gtatggacga actggaaggt catgagtttg
    24841 atctcattat taatgcaaca tccagtggca tcagtggtga tattccggcg atcccgtcat
    24901 cgctcattca tccaggcatt tattgctatg acatgttcta tcagaaagga aaaactcctt
    24961 ttctggcatg gtgtgagcag cgaggctcaa agcgtaatgc tgatggttta ggaatgctgg
    25021 tggcacaggc ggctcatgcc tttcttctct ggcacggtgt tctgcctgac gtagaaccag
    25081 ttataaagca attgcaggag gaattgtccg cgtgaatcag gccatccagt ttccggacag
    25141 ggaagagtgg gacgagaata aaaaatgtgt atgttttccc gctctcgtga atggtatgca
    25201 actgacatgc gcgatctctg gcgagagtct ggcgtatcgc tttactggag atacgccaga
    25261 acagtggtta gcgagttttc gtcagcatcg ctgggacctg gaagaagaag cggaaaactt
    25321 aattcaggaa caaagtgaag atgatcaagg ttgggtctgg ttaccctgat ccaggtattc
    25381 gtctttccat ttcacgtaat tattcgcgga gtaacgtaac ccagccttct cttcatcact
    25441 taacgggcgg atctgtttga cgggactacc gagatacaga tatccgctct ccagccgttt
    25501 attttgtggg accagactac ccgcaccaat catcacatca tcttctacta ttgcgccatc
    25561 aagtaaaatt gagcccatcc caaccaaaac tcgattgcca atggtgcagc cgtggagcat
    25621 caccttgtga ccaacagtga catcttcgcc aatggttaat gggttgccat ctgggttgta
    25681 cgaggattta tgagtgacat gcaacatact gccatcctgg atattggtgc gtgctccgat
    25741 ctgtacataa tgtacatctc cacgaatcac aacgagcggc cagatcccca catcatcagc
    25801 cagacgaacg tcaccaatca cgacactgct atcgtcgatc attacgcgct gaccgatttg
    25861 tggaaaaaga tcgcggtatg ggcgtaaaac atcagacata cttacctcag caataaatga
    25921 tttactaatg actttggggg cattattggc cttgtgcaag tcttttagta tgcaaaaaag
    25981 caccgttttg tgtgcgattg cagcaaaaag ggtgaaaaaa caacaaacag aaaaaaagat
    26041 caaaaaaata cttgtgcaaa aaattgggat ccctataatg cgcctccgtt gagacgacaa
    26101 cgtgaaacac ttcacaggat ggtcggaaca acgaagag
//
LOCUS       DAZFFG010000069        25604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFFG010000069 DAZFFG010000000
VERSION     DAZFFG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.069"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(105..800)
                     /gene="aqpZ"
                     /locus_tag="ACHJ7J_003082"
     CDS             complement(105..800)
                     /gene="aqpZ"
                     /locus_tag="ACHJ7J_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO5001283.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1226..2884
                     /gene="ybjD"
                     /locus_tag="ACHJ7J_003083"
     CDS             1226..2884
                     /gene="ybjD"
                     /locus_tag="ACHJ7J_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO5001284.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HICRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2881..3837)
                     /gene="ybjX"
                     /locus_tag="ACHJ7J_003084"
     CDS             complement(2881..3837)
                     /gene="ybjX"
                     /locus_tag="ACHJ7J_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO5001285.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAADLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3988..5103
                     /gene="macA"
                     /locus_tag="ACHJ7J_003085"
     CDS             3988..5103
                     /gene="macA"
                     /locus_tag="ACHJ7J_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO5001286.1"
                     /translation="MKKRKTVKKRYLIALVIAIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5100..7046
                     /gene="macB"
                     /locus_tag="ACHJ7J_003086"
     CDS             5100..7046
                     /gene="macB"
                     /locus_tag="ACHJ7J_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO5001287.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNIDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSSEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7119..7343)
                     /gene="cspD"
                     /locus_tag="ACHJ7J_003087"
     CDS             complement(7119..7343)
                     /gene="cspD"
                     /locus_tag="ACHJ7J_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO5001288.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7493..7567
                     /gene="yljB"
                     /locus_tag="ACHJ7J_003088"
     CDS             7493..7567
                     /gene="yljB"
                     /locus_tag="ACHJ7J_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO5001289.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7666..7986
                     /gene="clpS"
                     /locus_tag="ACHJ7J_003089"
     CDS             7666..7986
                     /gene="clpS"
                     /locus_tag="ACHJ7J_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO5001290.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8017..10293
                     /gene="clpA"
                     /locus_tag="ACHJ7J_003090"
     CDS             8017..10293
                     /gene="clpA"
                     /locus_tag="ACHJ7J_003090"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO5001291.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10637..10724)
                     /locus_tag="ACHJ7J_003091"
     tRNA            complement(10637..10724)
                     /locus_tag="ACHJ7J_003091"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10688..10690),aa:Ser,seq:gga)
     gene            complement(10978..11196)
                     /gene="infA"
                     /locus_tag="ACHJ7J_003092"
     CDS             complement(10978..11196)
                     /gene="infA"
                     /locus_tag="ACHJ7J_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO5001292.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11481..12185)
                     /gene="aat"
                     /locus_tag="ACHJ7J_003093"
     CDS             complement(11481..12185)
                     /gene="aat"
                     /locus_tag="ACHJ7J_003093"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO5001293.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12227..13948)
                     /gene="cydC"
                     /locus_tag="ACHJ7J_003094"
     CDS             complement(12227..13948)
                     /gene="cydC"
                     /locus_tag="ACHJ7J_003094"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO5001294.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13949..15715)
                     /gene="cydD"
                     /locus_tag="ACHJ7J_003095"
     CDS             complement(13949..15715)
                     /gene="cydD"
                     /locus_tag="ACHJ7J_003095"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO5001295.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15838..16803)
                     /gene="trxB"
                     /locus_tag="ACHJ7J_003096"
     CDS             complement(15838..16803)
                     /gene="trxB"
                     /locus_tag="ACHJ7J_003096"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO5001296.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17348..17842
                     /gene="lrp"
                     /locus_tag="ACHJ7J_003097"
     CDS             17348..17842
                     /gene="lrp"
                     /locus_tag="ACHJ7J_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO5001297.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17977..22083
                     /gene="ftsK"
                     /locus_tag="ACHJ7J_003098"
     CDS             17977..22083
                     /gene="ftsK"
                     /locus_tag="ACHJ7J_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO5001298.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAIP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQRPQQPVAPQPQYQQPQQSAAPQQQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22238..22849
                     /gene="lolA"
                     /locus_tag="ACHJ7J_003099"
     CDS             22238..22849
                     /gene="lolA"
                     /locus_tag="ACHJ7J_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO5001299.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22860..24203
                     /gene="rarA"
                     /locus_tag="ACHJ7J_003100"
                     /gene_synonym="mgsA"
     CDS             22860..24203
                     /gene="rarA"
                     /locus_tag="ACHJ7J_003100"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO5001300.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24294..25586
                     /gene="serS"
                     /locus_tag="ACHJ7J_003101"
     CDS             24294..25586
                     /gene="serS"
                     /locus_tag="ACHJ7J_003101"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO5001301.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         6438 a         6367 c         6861 g         5938 t
ORIGIN      
        1 tgcgccagtg actatatgtg gcttaacaca tcaaggagcg caacaggaag atgaatcaat
       61 atatttaaaa aaggcctgac attgcgccag gccttctgcg ttaattaatt acgcttttcc
      121 agcagggtcc ggtaaatcag accaccaata ataccgccga caattggcac cacccagaag
      181 aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc
      241 gggttaacag aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga
      301 ccaatagcga tcggcgcaaa acctgccgga gcgaatttgt cggttgcgcc gtggatcacc
      361 aacaggaaac ctgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg
      421 cctggtgaat gctcgccata accgttagaa gcaaaaccgc tggctgccgc gtcaaaaccc
      481 gttttaccac tggcaattaa atacagcagc gctgccgcaa caataccgcc gacaacctgg
      541 gcaattacgt agccaacgac ttcttttgcc ggaaaacgtc cgccagccca taaaccaata
      601 gtgaccgccg ggttaaaatg accaccagaa atatgaccaa cagcgaaggc catcgtcaga
      661 acggtcaaac cgaatgccag cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct
      721 gcggccagta cagcactacc acagccacca aaaacaagcc agaaagtacc aaaacattca
      781 gctgctaatt ttctgaacat atccacctca attaaaaatt gaccctgtga aaaatatggt
      841 cgttttatag ggtcgtcgta aaaaagtgac gacgcaaata atgcgcggct attttaaaaa
      901 cgaaggcgag tcattcacca gataaataaa tccagtaaat ttgatttagg gcaacagcgg
      961 gttgccccat atagtcattt gtctgattga cagtgtagtg caagcaaaag atttaatcct
     1021 ttaggcgtaa taaaaaataa tttatcatgc taattatttg attttgttgt ttttgcagac
     1081 ttatcagcaa gagggagtat aacgcgatta ttcgctcatt tttcagacat ttgccatgct
     1141 taaatgtgat gtcatcacgt attagcaagg cctttcccgt tatactgcca gcgtaaagga
     1201 taagtcacat atttctggag gggatatgat tcttgagcgc gttgaaattg tgggttttcg
     1261 cggtatcaac cgtttgtcgt tgatgctgga acaaaacaac gtcctgattg gggagaacgc
     1321 gtggggtaaa tccagcttgc tggacgcctt aaccctgctg ctatcgccag aatcagatct
     1381 ctaccatttt gagcgcgacg atttctggtt cccgccggga gatatcaacg ggcgagaaca
     1441 tcatctgcat attattttga ccttccgcga atcgctgcca ggccgacatc gggttcgccg
     1501 ttatcggccg ctggaagcgt gctggacgcc atgcaccgat ggctatcacc gtatttttta
     1561 tcgtctggaa ggggagagtg cggaagacgg cagcgtgatg acactgcgca gttttctcga
     1621 taaagacgga catccgattg atgttgagga tattaacgat caggcacgcc atctggtgcg
     1681 tttaatgccg gtgctgcgct tgcgtgatgc ccgttttatg cgccgtattc gtaacggcac
     1741 ggtgccaaat gtccccaatg tggaagtcac cgcgcgccag ctcgatttcc tcgcccgtga
     1801 gttgtcctca catccgcaaa atctctctga tgggcagatt cgtcagggac tttccgcaat
     1861 ggtgcagttg cttgagcatt atttctctga gcagggggct ggacaggcgc gatatcgttt
     1921 aatgcggcgg cgggccagca atgagcaacg aagctggcgc tatctggaca tcatcaaccg
     1981 gatgattgac cgacctggag ggcgctcgta tcgggttatt ttgctcggcc tgtttgctac
     2041 tttattgcag gcaaaaggca cattgcgact ggataaagac gcccgtccat tgttgctgat
     2101 cgaagatcca gaaacgcgtt tacaccccat tatgctttca gttgcctggc atctgttgaa
     2161 tcttctgccc ttgcagcgca ttgccaccac caactcgggt gagttacttt cgttaacgcc
     2221 ggtagagcat atttgccgac tggtacgtga gtcctcgcgc gttgccgcct ggcgtctggg
     2281 gccaagtggc ttgagcaccg aagacagccg ccgcatctct tttcacattc gttttaatcg
     2341 tccgtcatcg ctgtttgctc gctgctggtt gctggtggaa ggggaaacgg aaacctgggt
     2401 tatcaatgaa ctggcgcgtc agtgcggaca tcattttgat gccgaaggga tcaaggtcat
     2461 tgagtttgcc cagtccgggc taaagccact ggttaaattt gcccggcgaa tggggattga
     2521 atggcatgta ctggtcgatg gcgatgaagc agggaagaaa tatgccgcta cggtacgcag
     2581 cctgttgaat aatgatcggg aagccgaacg agaacattta acggcgttac cggcgctgga
     2641 tatggaacat tttatgtatc gccagggatt ttccgatgtg ttccaccgcg tggcgcaaat
     2701 cccggaaaat gtcccgatga atctgcgcaa aattatctcg aaagcgatcc atcgctcttc
     2761 caaacccgat cttgccattg aagtggcaat ggaggccgga cgtcgtggtg tggactccgt
     2821 accgacgctg ctgaaaaaaa tgttctcacg cgtgctgtgg ctggcgcgcg gtcgcgcgga
     2881 ttaaccgcga aacatcgtgg ccatttgtgg ctgaatagcg tcgagcatct catagcgccg
     2941 acggtattca gcccgttttt tactggcgat ttcggcaatc tcttttcgtg ctatctgtgc
     3001 tggaaggcgg taatggcgtt cagcatcaca tacgccgcca accgattccc agaaagcgtt
     3061 gtaatcggca tggatcttgc cttctttatc gcgataacgc aggctgcggt aaatatgcgt
     3121 ttcattgctg acggcaataa tctgctctac ctgcaaacgt tgggcaaaca gacaggccgc
     3181 ttccatcacg aggcgtttgg gaaatagccc gtggcaggct ttcgtcgcat tctggatttc
     3241 ctgatgtgga atttcccatt ttgcgccttg cagtccacca ataaacatcg ttcttttccc
     3301 ctgatattca cacagggtaa acgtgatttc tgccagagga ataccttcgc tgttgcggaa
     3361 caggattgtg ctgtcacctt ctttatccat tgagatcatc atggtcagct caagcgtgaa
     3421 ctgctcgccg tttttgcctt ccagcttcgc cagttgcagc ccgggggtat tcaaatataa
     3481 gctgaattct tccgccgaca tacacccgcg gagtaacgca taatggtaac gtaacgcctc
     3541 cagcaattgc ttacggctaa gatccgccgc aaggtaaggg cgatgcagac gtacaggcag
     3601 tcgcggctgg cgcgttaaca atacattgag attaggccag tgggaaagtt cgttcatcca
     3661 ctcaacgctt aaacgcggca taatcaacga gcgcagcaaa aatttctggc gaaaactacg
     3721 gcgatgccag aatttacccg gccgacactg tccacgtgcc agactaagaa aaagtgacag
     3781 gctgctgaga gattcagatg gcgtaaaggt ccgttcagtt agctgcgaca tattcatgaa
     3841 atcaatggtt atacatgacg tcgatttcac cattgcgtat cttaaccaaa catcaatagt
     3901 gtgattacta acgtaaattt tagggttttg ttgatatttc gttgaagtta atgacccgga
     3961 ttggcatatg gagtattcag aatatttatg aaaaagcgga aaaccgtgaa gaagcgttac
     4021 cttattgcgc tggtgatagc catcgccgga ctgattacgt tatggagaat tcttaacgca
     4081 cccgtgccga cttatcagac actgattgtg cgccccggtg atttacagca aagcgtgctg
     4141 gcgaccggaa agctggacgc gctgcgtaag gttgatgtgg gcgcgcaggt cagcggtcag
     4201 ttgaaaacac tgtcggtggc gattggcgat aaagtaaaaa aagaccagct tttaggggtc
     4261 attgatcctg aacaggctga aaaccagatc aaggaggtcg aagcaacgct gatggagcta
     4321 cgcgcgcagc ggcagcaggc ggaagcggag ctgaaactgg cgcgggtgac gtattcccgt
     4381 cagcaacgtc tggcacaaac gcaggcggtt tcacagcagg atctcgacac cgccgcgacg
     4441 gagatggctg tgaaacaggc gcaaattggc accattgacg cgcaaatcaa gcgcaatcag
     4501 gcttctctcg atacggctaa aaccaatctc gattacactc gcatcgttgc cccgatggcc
     4561 ggggaagtca cgcaaatcac cactctgcaa ggccagacgg tgattgccgc acaacaagca
     4621 ccgaacattc tgacgctggc agatatgagc accatgctgg taaaagcgca ggtttctgaa
     4681 gcggatgtaa tccacctgaa gccggggcaa aaagcctggt ttacggtact tggcgatcca
     4741 ctgacgcgct acgaggggca aatcaaggat gtactaccga cgccggaaaa ggttaacgac
     4801 gctattttct attacgcccg ttttgaagtc cccaacccca atggtttgct gcggctggat
     4861 atgactgcgc aagtgcatat tcagctcacc gatgtgaaaa atgtgctgac gatccctctg
     4921 tcggcgttag gcgatccggt tggcgataat cgttataaag tcaaattgtt gcgtaatggt
     4981 gaaacacgcg agcgtgaagt gacgattggc gcacgtaacg ataccgatgt tgagattgtc
     5041 aaagggctgg aagcgggcga tgaagtggtg attggtgagg ccaaaccagg agctgcacaa
     5101 tgacgccttt gctcgaatta aaggatattc gtcgcagcta tcctgccggt gatgagcagg
     5161 ttgaggtgct gaagggcatc accctcgata tttatgctgg tgagatggtg gcgattgttg
     5221 gcgcttcggg ttccggtaaa tcgaccctga tgaatattct cggctgtctg gataaggcca
     5281 ccagcggcac ctatcgcgtc gccggtcagg atgttgccac gctggacgcc gatgcgctgg
     5341 cgcaactgcg ccgcgagcat ttcggcttta ttttccagcg ttaccatttg ctttcgcatt
     5401 taaccgccga gcagaacgtt gaagtacccg ccgtctatgc cggtcttgag cggaaacagc
     5461 ggctgcttcg cgcccaggag ttgctgcaac ggctggggct ggaagaccgt acagagtatt
     5521 atccggcaca gctttcgggc ggtcagcaac agcgcgtcag catcgcgcgg gcattgatga
     5581 acggcggtca ggtaattctt gccgatgaac caaccggcgc actggacagc cattctggcg
     5641 aagaggtgat ggcgatcctg catcagctgc gcgatcgcgg acatacggtg attatcgtca
     5701 cccacgatcc gcaggtcgct gctcaggccg agcgggtgat cgaaattcgc gacggcgaaa
     5761 ttgtgcgcaa tcctcccgcc attgaaaaag tgaatgttgc tggcgggacg gaacctgttg
     5821 tcaacacggt gtctggctgg cggcagtttg tcagcggttt taacgaggcg ctgacgatgg
     5881 catggcgggc gctggcagcg aataaaatgc gtactttact gaccatgctg gggattataa
     5941 tcggtattgc gtcggtggtt tccattgtcg tggtgggcga cgccgccaaa caaatggtgt
     6001 tggcggatat tcgttctatt ggtacgaata ctattgatgt ctatcccggg aaagattttg
     6061 gcgatgacga tccgcaatat cagcaggcgc tgaagtacga tgacttaatc gccatccaaa
     6121 aacaaccgtg ggtcgcttca gccacacctg ccgtctcgca aaacctgcgc ctgcgttata
     6181 acaatattga tgttgctgcc agtgccaatg gcgtgagcgg cgattatttt aatgtctatg
     6241 gcatgacctt cagtgaagga aacaccttta accaggaaca gctgaacggt cgtgcgcagg
     6301 tcgtggttct cgacagtaat actcgccgcc agcttttccc ccataaagca gatgtggttg
     6361 gcgaggtgat tctggtcggc aatatgcccg ccagagtcat tggtgtggcg gaagaaaaac
     6421 agtcgatgtt tggtagcagt aaagtgctgc gtgtctggct accttacagc acgatgtccg
     6481 ggcgagttat gggccagtcg tggctgaact ccattactgt cagggtgaaa gaaggatttg
     6541 acagttccga ggcggaacag caactcacgc gtttactttc actgcgccac ggtaagaagg
     6601 atttctttac ctggaacatg gacggcgtct tgaaaaccgt tgaaaagacc acacgtactt
     6661 tacaactgtt tctgacgctg gtggcggtga tttcgctggt agtgggcggt attggtgtaa
     6721 tgaatattat gctggtgtca gtgaccgagc ggacgcggga aattggcatt cgcatggctg
     6781 taggtgcgcg agcaagcgat gttttgcaac agttcctgat cgaagccgta ctggtttgcc
     6841 tggtcggtgg cgcgttggga ataacactgt cactgttaat tgctttcacc ttgcagcttt
     6901 tcttacccgg ctgggagatt ggtttttcac cgttggcact gctgctggcg tttctctgct
     6961 cgacggtcac cgggatttta tttggctggt tacccgcacg aaatgcggca cgactggatc
     7021 cagtagatgc tctggcacga gagtaatttt tgagataaaa atgccagccg atcgggctgg
     7081 cattttgcct ttaggatgta cacaatgaga cagaagagct atgcgactgc cgcttctact
     7141 tcgacgggca caataacact ggcgtgattg ccttttggcc cctggtggac atcaaactga
     7201 acggattgtc cagcttttag cgttctgtaa ccatccatct gaatggtgga ataatgagcg
     7261 aaaatatctt cgccgccgcc ttcagggcag atgaaaccaa accctttggc attgttgaac
     7321 cacttaacag tacccttttc catgcttcga catccttcgc aaatcttata caagtaagat
     7381 ggaataaacc ggggtcagag agggggctgt tcaaaacctc gccaactcta gaaatacaat
     7441 ttagagaatt agggcgagcc gtcaagcatt tgacaggggc cagggggcag gtatgaatca
     7501 aaaatttgaa gcagttaacg ctattgacag gaatgtgaca gatgtcgctg atgccaacga
     7561 tagatgatag ttatctatca tgtggagtag attggtcagg caaataagct cttgtcagcg
     7621 gcagggcgtt ctgccgataa ccgtaaccga agatgataac tgacaatggg taaaacgaac
     7681 gactggctgg actttgatca actggcggaa gaaaaagttc gcgacgcgct aaaaccgcca
     7741 tctatgtata aagtgatatt agtcaatgat gattacactc cgatggagtt tgttattgac
     7801 gtgttacaaa aattcttttc ttatgatgta gaacgtgcaa cgcaattgat gctcgctgtt
     7861 cactaccagg ggaaggccat ttgcggagtc tttaccgccg aggttgcaga aaccaaagtg
     7921 gcgatggtga acaagtacgc gagggagaat gagcatccat tgctgtgtac gctagaaaaa
     7981 gcctgaatgc aggcataaaa attgggggag gtgcctatgc tcaatcaaga actggaactc
     8041 agtttaaata tggctttcgc cagagcgcgc gagcaccgtc atgagtttat gaccgtcgag
     8101 cacttgttac tggcgctgct cagtaaccca tctgcccggg aggcgctgga agcgtgttct
     8161 gtggatttgg ttgcgctccg tcaggaactg gaagccttta ttgaacaaac cacacccgtt
     8221 ctgcctgcca gtgaagagga gcgcgacaca cagccgacgc tgagttttca gcgtgtactg
     8281 caacgtgcgg tcttccatgt ccagtcctcc ggtcgcaatg aggttaccgg tgcaaacgtt
     8341 ctggtcgcta tctttagcga acaggagtcg caggcggcat atctgttgcg taaacatgaa
     8401 gtcagccgtc tcgatgtggt gaactttatc tctcatggca cgcgtaaaga cgagccgaca
     8461 cagtcttctg atcctggcag ccagccaaac agcgaagaac aagctggtgg ggaggaacgt
     8521 atggagaatt tcacgacgaa cctgaatcag cttgcgcgcg tgggcggaat cgacccactg
     8581 attggtcgtg agaaggagct ggagcgtgct attcaggttc tctgccgtcg ccgtaaaaac
     8641 aacccgctgc tggtggggga atctggtgtc ggtaaaaccg cgattgcaga aggtcttgcc
     8701 tggcgaattg ttcagggcga tgtgccggaa gtgatggctg actgtacgat ttactctctc
     8761 gatatcggtt ctctgttagc gggcacaaaa tatcgcggcg actttgaaaa acgttttaaa
     8821 gcgttgctca agcagctgga gcaggacact aacagcatcc tgtttattga tgagatccac
     8881 accattatcg gtgcgggtgc agcgtctggt ggtcaggtcg atgcggctaa cctaatcaaa
     8941 ccgttgctct ccagcggtaa aattcgcgta attggttcga caacctatca ggagttcagc
     9001 aacattttcg agaaagaccg tgctctggcg cgtcgcttcc agaaaattga tattactgaa
     9061 ccgtcgatcg aagaaactgt tcaaatcatc aatggcctga aaccgaagta tgaagcgcac
     9121 cacgacgtgc gttataccgc aaaagcggtg cgtgcggcgg tagagctggc ggtgaaatac
     9181 attaacgatc gtcatctgcc ggataaagcg attgatgtta tcgacgaagc gggcgctcgc
     9241 gcacgcctga tgccggtaag caaacgcaag aaaaccgtaa atgtggcgga tattgagtcc
     9301 gtggtggccc gtattgcacg cattccagag aagagtgttt ctcagagtga ccgcgatacc
     9361 ctgaaaaacc tcggcgatcg cctgaaaatg ctggtcttcg gtcaggataa agctattgag
     9421 gcgctgactg aagccattaa gatggcgcgt gcaggtttag gtcacgaaca taaaccggtt
     9481 ggttcgttcc tgtttgccgg ccctaccggg gtcgggaaaa cagaggtgac ggtacagctt
     9541 tcgaaagctt tgggcattga gcttctgcgc tttgatatgt ccgagtatat ggaacgccat
     9601 accgtcagcc gtcttattgg tgcgcctccg ggatacgttg gttttgatca gggcggtttg
     9661 ctgactgatg cggtcatcaa gcatccacat gcggtgctgc tgctggacga aatcgagaaa
     9721 gcgcacccgg acgtgttcaa tattctgttg caggtgatgg acaacggtac gctgaccgat
     9781 aacaacggac gcaaagcgga cttccgtaac gtggtgctgg tgatgaccac caacgccggg
     9841 gtacgggaaa ctgagcgtaa atccattggt cttatccacc aggataacag caccgatgcg
     9901 atggaagaga tcaagaagat ctttacaccg gaattccgta accgtctcga caacattatc
     9961 tggttcgatc atctgtcaac tgacgtgatc catcaggtgg tggataaatt catcgtcgag
    10021 ttgcaggttc agctggatca gaaaggtgtt tcgctggaag tgagccagga agcgcgtaac
    10081 tggctggccg agaaaggtta cgaccgggca atgggcgcac gtccgatggc gcgtgtcatc
    10141 caggacaacc tgaaaaaacc gcttgccaac gaactgctgt ttggttcgct ggtggacggc
    10201 ggtcaggtca ccgtcgcgct ggataaagag aaaaatgagc tgacttacgg attccagagt
    10261 gcacaaaagc acaaggcgga agcagcgcat taatctgatt gttaagtagg ttggtgaagt
    10321 ccgtaatctc gaaagaggtt acggactttt tatttatggg ggaggaggtt cagacccttt
    10381 ttttgatgat gatggtaagt tgttgataat tagtgctgcg gtaaggtaag gataaaaaaa
    10441 gggtggtagc aggaaaatga gatggttttg ctttattaac aacgggctaa acgtgtagta
    10501 tttgtgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc
    10561 accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca ctttcgtacg
    10621 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    10681 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctgcc
    10741 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    10801 aattttaatg ataattattg tttgctcata ctgtaaacaa gttgtgcagt atatctacat
    10861 cgagacaagt tacggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    10921 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    10981 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    11041 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    11101 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    11161 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    11221 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    11281 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    11341 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    11401 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    11461 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcatcgtgg tacccagaaa
    11521 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc ccggcgggga
    11581 atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta
    11641 ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc
    11701 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    11761 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    11821 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    11881 cagccttcaa tcacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    11941 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    12001 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    12061 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    12121 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    12181 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    12241 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    12301 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    12361 tgaccattaa caccgttttc tcacgcatca tttctgcaag caattcaagg atctgacttt
    12421 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    12481 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    12541 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgca
    12601 agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    12661 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    12721 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    12781 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    12841 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    12901 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    12961 gggtatcagg gaaggtgacc tccggttttt gatccgttaa atcagtgatc cgaacggcag
    13021 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    13081 cggctaacgc gcagaagaca aacagggcaa ttaacgcgcc gggttgagca ttgccgccaa
    13141 cgccgccaga cgccatccac agcatcagga tcaccgctaa tgcgccaatg agcagcatta
    13201 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    13261 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    13321 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    13381 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    13441 taatgccgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    13501 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    13561 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    13621 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    13681 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    13741 ctgcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    13801 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    13861 gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    13921 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    13981 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    14041 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    14101 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    14161 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    14221 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    14281 caggcgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    14341 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgta
    14401 atgttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    14461 ctggtgataa atcgcgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    14521 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcgacca accaacactg
    14581 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    14641 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggttgatgct aattccgtct
    14701 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    14761 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    14821 ctggcgcaag gatcagggcc agaaaacccg cagccagcgt cacgccagta tcgtagtgac
    14881 caaaatccag ctcgccgaga taggaaaaac caaagtagac tgccaccaga gcaattgaca
    14941 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc acttccattg
    15001 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    15061 caaaaatacg cagtgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    15121 caagagcgag aaagttacgt cggttagcat cggcagcccc cattccaacc agcgccataa
    15181 acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    15241 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcaggtagc
    15301 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    15361 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    15421 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    15481 cccatgcgcg cagtacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    15541 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccat gcctgggcaa
    15601 tgatcaatat gccgctcaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    15661 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    15721 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    15781 aggcgaccca tagtcgcatg gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    15841 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagcctgt
    15901 accagcagat gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    15961 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgcactt tgatgtagcc
    16021 gttttccagt tccagctgcc cttcgaaaat ggcagtattc gggctgtgac cgatagcaac
    16081 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    16141 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    16201 aatgatgttg ccgttctcca ctttatccat cagacgctta atgaggattt tttccgcgcg
    16261 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    16321 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    16381 accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag
    16441 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    16501 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    16561 aatgatctca gtttcaaatt tggtggcatg ttcgtgcatg cgctccatta ataacggacc
    16621 ggtcagatcg tttggatcgc caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    16681 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc
    16741 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    16801 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    16861 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    16921 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    16981 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aatcgatgtt
    17041 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    17101 cttatatgcg cataaccatg catgtaaata ccatgtttac cgtgctagtg aaatctacgt
    17161 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    17221 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    17281 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    17341 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    17401 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    17461 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    17521 tcagggctat acggcgctgc tgaaccccca ttatctggat gcatcacttc tggtattcgt
    17581 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    17641 aaaacttgaa gaaattcagg agtgtcattt agtatccggt gatttcgact acctgttgaa
    17701 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    17761 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    17821 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    17881 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    17941 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    18001 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    18061 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    18121 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    18181 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    18241 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga atacattgat
    18301 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    18361 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    18421 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    18481 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    18541 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    18601 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    18661 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    18721 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    18781 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgccgac
    18841 ccggacgacg tcctattttc gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    18901 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    18961 gcgacgcaaa gctgggctgc accggttgaa cctgtgactc agacgccacc tgttgcctct
    19021 gttgatgttc cacctgcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    19081 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    19141 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    19201 gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct
    19261 gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc gtattatgcc
    19321 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccact
    19381 tttgctccac agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag
    19441 ccgttgtacc aacagccgca acctgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    19501 gtagaagaga caaaacccgc gcgtccgccg ctttactact ttgaagaagt ggaagagaag
    19561 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    19621 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcaat acctccagta
    19681 gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    19741 ggggctgccg caaccgttgc tgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    19801 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    19861 actcgtcgtg aactggcgtc ttacggtatt aaactgccct cacagcgtgc ggcggaagaa
    19921 aaagcccgtg aagcccagcg caatcagtat gattctggcg atcagtataa tgatgatgaa
    19981 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    20041 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    20101 gaggctgaac tggctcgtca gtttgcgcaa actcaacaac aacgttattc cggcgaacaa
    20161 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    20221 ctggatgatg gtccacacga accgttgttt acgccaattg ttgaacctgt acagcagccg
    20281 caacaaccgg ttgcaccgca gcagcagtat cagcagccac aacagccggt tgcgccacag
    20341 cagcaatatc agcagccgca acaaccggtt gcaccgcagc agcaatatca gcagccgcaa
    20401 caaccggttg caccgcagcc gcaatatcag cggccgcaac agccagttgc gccgcagccg
    20461 cagtatcagc agccacaaca atcggctgca ccacagcagc aatatcagca gccgcaacag
    20521 ccagttgcgc cacagccgca ggatactctg cttcatccgc tgttgatgcg taatggcgac
    20581 agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    20641 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg tctggtggaa
    20701 gcacgtctgg ctgatttccg tattaaagcc gatgtcgtca attactctcc ggggccggtt
    20761 atcactcgct ttgaacttaa cctggcaccg ggcgtaaaag cggcgcgcat ttctaacttg
    20821 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    20881 ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt ttatctgcgc
    20941 gaagtattgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    21001 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    21061 gcggggacta ccggttccgg taagtctgtc ggtgtgaacg cgatgatcct gagcatgctt
    21121 tataaagcgc agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    21181 tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttactgatat gaaagatgcc
    21241 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc ggtataaact gatgtctgcg
    21301 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    21361 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    21421 ctgaaaaaag agccatacat tgtggtgttg gttgacgaat ttgccgacct gatgatgacg
    21481 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    21541 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    21601 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    21661 cttgatcagg ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg
    21721 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    21781 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac
    21841 agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc
    21901 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttcagg cgtacagcgt
    21961 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    22021 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    22081 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    22141 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    22201 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ccatcacctg
    22261 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    22321 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    22381 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    22441 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    22501 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    22561 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    22621 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    22681 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    22741 tcagcgcagc agttatcaac tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    22801 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    22861 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    22921 ggccagaaaa tttagcacag tatatcggtc agcaacattt gctggctgcg gggaagccgt
    22981 tgccgcgcgc tatcgaagcc gggcatttac attctatgat cctctggggg ccaccgggta
    23041 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    23101 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    23161 accgcaatgc aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa
    23221 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    23281 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc
    23341 tgttgaaatc cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    23401 aaacccgtgg ctatggtggt caggatattg ttctgccaga tgaaacacga cgtgccattg
    23461 cagaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    23521 tggccgaagt cgatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg
    23581 ccggtgaacg tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctgatttctg
    23641 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa
    23701 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg
    23761 aagacgtcgg taatgccgat ccacgagcga tgcaggtggc aattgcggcc tgggattgct
    23821 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    23881 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc
    23941 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    24001 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    24061 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    24121 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    24181 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    24241 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    24301 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    24361 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    24421 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    24481 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    24541 atgcagcaaa agccgagctg gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    24601 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    24661 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    24721 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    24781 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    24841 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    24901 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    24961 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    25021 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    25081 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    25141 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccggaa gactcaatgg
    25201 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    25261 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    25321 tggaagtatg gatcccggca cagaacacct accgcgagat ctcttcctgc tccaacgttt
    25381 gggatttcca ggcacgtcgt atgcaggcac gttgtcgcag caagtcggac aagaaaaccc
    25441 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcggtta
    25501 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    25561 tgaacggact ggaatatatt ggctaatacc taatttttct gaat
//
LOCUS       DAZFFG010000070        25137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFFG010000070 DAZFFG010000000
VERSION     DAZFFG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.070"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..212
                     /gene="ypdI"
                     /locus_tag="ACHJ7J_003102"
     CDS             <1..212
                     /gene="ypdI"
                     /locus_tag="ACHJ7J_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO5001302.1"
                     /translation="AFSISGGGSERSYKETEKTSAMTTTHSTKLQPSQAILFKMREDA
                     PPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(289..531)
                     /gene="yfdY"
                     /locus_tag="ACHJ7J_003103"
     CDS             complement(289..531)
                     /gene="yfdY"
                     /locus_tag="ACHJ7J_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO5001303.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            884..1804
                     /gene="lpxP"
                     /locus_tag="ACHJ7J_003104"
     CDS             884..1804
                     /gene="lpxP"
                     /locus_tag="ACHJ7J_003104"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO5001304.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2160..2231
                     /gene="ypdK"
                     /locus_tag="ACHJ7J_003105"
     CDS             2160..2231
                     /gene="ypdK"
                     /locus_tag="ACHJ7J_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO5001305.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2296..3534)
                     /gene="alaC"
                     /locus_tag="ACHJ7J_003106"
     CDS             complement(2296..3534)
                     /gene="alaC"
                     /locus_tag="ACHJ7J_003106"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO5001306.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3910..5607
                     /gene="ypdA"
                     /locus_tag="ACHJ7J_003107"
     CDS             3910..5607
                     /gene="ypdA"
                     /locus_tag="ACHJ7J_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO5001307.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTELGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5622..6356
                     /gene="ypdB"
                     /locus_tag="ACHJ7J_003108"
     CDS             5622..6356
                     /gene="ypdB"
                     /locus_tag="ACHJ7J_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO5001308.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6369..7226
                     /gene="ypdC"
                     /locus_tag="ACHJ7J_003109"
     CDS             6369..7226
                     /gene="ypdC"
                     /locus_tag="ACHJ7J_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5001309.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVCFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQQLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7229..9724)
                     /gene="ptsP"
                     /locus_tag="ACHJ7J_003110"
     CDS             complement(7229..9724)
                     /gene="ptsP"
                     /locus_tag="ACHJ7J_003110"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5001310.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9749..10786)
                     /gene="ypdE"
                     /locus_tag="ACHJ7J_003111"
     CDS             complement(9749..10786)
                     /gene="ypdE"
                     /locus_tag="ACHJ7J_003111"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5001311.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGVVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10786..11871)
                     /gene="ypdF"
                     /locus_tag="ACHJ7J_003112"
     CDS             complement(10786..11871)
                     /gene="ypdF"
                     /locus_tag="ACHJ7J_003112"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5001312.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESEHILVDSRYYADVEARTQGYQLHLLDATHTLATIVNEIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11886..13133)
                     /gene="fryC"
                     /locus_tag="ACHJ7J_003113"
     CDS             complement(11886..13133)
                     /gene="fryC"
                     /locus_tag="ACHJ7J_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5001313.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13155..13481)
                     /gene="fryB"
                     /locus_tag="ACHJ7J_003114"
     CDS             complement(13155..13481)
                     /gene="fryB"
                     /locus_tag="ACHJ7J_003114"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5001314.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAVVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13700..14665)
                     /gene="glk"
                     /locus_tag="ACHJ7J_003115"
     CDS             complement(13700..14665)
                     /gene="glk"
                     /locus_tag="ACHJ7J_003115"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO5001315.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPIEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14869..16125
                     /gene="yfeO"
                     /locus_tag="ACHJ7J_003116"
     CDS             14869..16125
                     /gene="yfeO"
                     /locus_tag="ACHJ7J_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO5001316.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16240..16566
                     /gene="ypeC"
                     /locus_tag="ACHJ7J_003117"
     CDS             16240..16566
                     /gene="ypeC"
                     /locus_tag="ACHJ7J_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO5001317.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16707..17942)
                     /gene="mntH"
                     /locus_tag="ACHJ7J_003118"
     CDS             complement(16707..17942)
                     /gene="mntH"
                     /locus_tag="ACHJ7J_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO5001318.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVMQGFIRFHIPLWVRRTVTMLPS
                     FIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVI
                     VVLVVALNIWLLVGTALGL"
     gene            18278..19480
                     /gene="nupC"
                     /locus_tag="ACHJ7J_003119"
     CDS             18278..19480
                     /gene="nupC"
                     /locus_tag="ACHJ7J_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO5001319.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19530..21719)
                     /gene="pdeA"
                     /locus_tag="ACHJ7J_003120"
     CDS             complement(19530..21719)
                     /gene="pdeA"
                     /locus_tag="ACHJ7J_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5001320.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(21927..22002)
                     /locus_tag="ACHJ7J_003121"
     tRNA            complement(21927..22002)
                     /locus_tag="ACHJ7J_003121"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21967..21969),aa:Ala,seq:ggc)
     gene            complement(22042..22117)
                     /locus_tag="ACHJ7J_003122"
     tRNA            complement(22042..22117)
                     /locus_tag="ACHJ7J_003122"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22082..22084),aa:Ala,seq:ggc)
     gene            22338..22697
                     /gene="yfeC"
                     /locus_tag="ACHJ7J_003123"
     CDS             22338..22697
                     /gene="yfeC"
                     /locus_tag="ACHJ7J_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5001321.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22699..23091
                     /gene="yfeD"
                     /locus_tag="ACHJ7J_003124"
     CDS             22699..23091
                     /gene="yfeD"
                     /locus_tag="ACHJ7J_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5001322.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23143..24558)
                     /gene="gltX"
                     /locus_tag="ACHJ7J_003125"
     CDS             complement(23143..24558)
                     /gene="gltX"
                     /locus_tag="ACHJ7J_003125"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO5001323.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            24817..24892
                     /locus_tag="ACHJ7J_003126"
     tRNA            24817..24892
                     /locus_tag="ACHJ7J_003126"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24850..24852,aa:Val,seq:tac)
     gene            24937..25012
                     /locus_tag="ACHJ7J_003127"
     tRNA            24937..25012
                     /locus_tag="ACHJ7J_003127"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24970..24972,aa:Val,seq:tac)
     gene            25059..25134
                     /locus_tag="ACHJ7J_003128"
     tRNA            25059..25134
                     /locus_tag="ACHJ7J_003128"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25092..25094,aa:Val,seq:tac)
BASE COUNT         6259 a         6656 c         6204 g         6018 t
ORIGIN      
        1 ttgcattttc catttctggt ggtggaagtg agaggagcta taaagagacg gaaaaaacat
       61 cagcgatgac gacaactcac tctacaaaac ttcagccatc acaggcaatt ttgtttaaga
      121 tgagagaaga tgcgccacca ttaaacctca cagaagaaac cacgccccct tttccgacaa
      181 aggcgaatta tcttattcat cctgtgcgat agcgtctgtc acatggcagg tgagcaatca
      241 tgtctgatgc tgatgtgaac acctgcccaa atgagccatc ctgacccact atcgcctgcc
      301 ataccccacc ataaagcgaa tcatcaagcc acaagccggg gcaagaaaca taaatatccc
      361 cgtcaccagt tcattaccgc ctgttaacca catggtgaag taataaatga gtgcagcgag
      421 gatcaccatg ccgaagaaag aagagaccgc cgataccacg ttcagtacct gaccgatgta
      481 tccgcttacg cagacaatac acagggcgag aaacatccat aaataaatca tagctcaacc
      541 tcctgccgcg ctaaaaaacc tgtaacttca gtcactttaa tagtaagaca atccttaccc
      601 tctggcaatt tttgctaagc atcaatgaaa acagcatgtt aaatgcaaga ctgttgtgta
      661 cggaaaaata tttactttgc acgattaata atcagtagct gaaagcagtc agcgagtgat
      721 aaagtacaga taccgcattc atgctcctcg tggttatgtc ctgactagtc tttacactct
      781 ttacaggaac cattgtcgta catgatggcc taatcaattg agtgtaccgc tgtcgacatg
      841 gaatcttctg cgatacaaca attcgtatct acagaaggta actatgtttc cacaatgcaa
      901 attttcccgc gagtttctgc atcctcgcta ctggctcaca tggtttgggc ttggtgtact
      961 ctggctttgg gtacagcttc cttatcctgt tctctgcttt ctcggcacgc gtattggcgc
     1021 aatggcgcga ccattcctga aacggcgtga atctatcgcc cgtaaaaacc tggaactttg
     1081 cttcccgcag cattctgcgg aagaacgcga gaagatgatt gccgaaaact ttcgttcact
     1141 cggcatggcg ctggtagaaa ccggcatggc gtggttctgg cccgacagtc gcgtacgtaa
     1201 atggtttgat gttgaagggc tggataacct taaacgcgca caaatgcaaa atcgcggcgt
     1261 aatggttgtc ggcgtccatt ttatgtcgct ggaactgggc ggccgcgtga tgggactttg
     1321 tcagccaatg atggctacct atcgtccaca taataatcag ctgatggaat gggtgcagac
     1381 ccgtggtcgc atgcgctcta acaaagcgat gatcggcaga aataatctgc gcggcattgt
     1441 cagtgcactg aagaaaggtg aagcggtatg gtttgctccc gatcaggatt atggtcgtaa
     1501 aggcagctcc ttcgcgccgt tctttgcggt agaaaatgtc gccacaacca atggcaccta
     1561 tgttctctcc cgtctctctg gcgcagccat gttgaccgta acgatggtaa gaaaagcgga
     1621 ttacagcgga tatcgtttgt tcatcacccc agagatggaa ggctacccga cagatgaaaa
     1681 tcaagccgct gcctatatga acaagattat cgagaaagaa atcatgcgcg caccggagca
     1741 gtacctctgg atccaccgtc gctttaaaac gcgcccggtg ggagaatcgt cgttatacat
     1801 ttaacaaact gctctgactc tttacctcct cacataagcc ataataacct catggttttt
     1861 atggcttttt tcttaaggtt ttttccttcg ctgaatatct aattattacc gtcagaaatt
     1921 aaactgtcgc cgatatgaga cacctgtaag ttactaaaag taaataagaa aatgcctctt
     1981 gtctcgtttc agttttaggc gtacattagc gccgtctcga gcagtttagc tcagaattct
     2041 ggggtgatgc ccacggtaaa atacaggact tttaaggtcg ttttgcccgg tgtcaactca
     2101 ctgtatcagg tgtaatgaag tcattcaggc gtaacagcaa ttacgcggag agatgtaaag
     2161 tgaaatattt ctttatgggc atttctttta tggtcatcgt ttgggccggt acttttgccc
     2221 tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc catcaggctc ctgtttgcat
     2281 ttctgaggtc ggtgtttatt ccgcgttttc gtgaatatgt ttgctgctgg cgggtagtaa
     2341 accgtcggca cggaacatcg ctttaatccc acgaatcgcc tgacgaatac ggtcgcggtt
     2401 ttcaatcagt gcaaagcgca catgcgtatc accatagtcg ccaaagccaa tccccggaga
     2461 gacacagacc ttcgcttcgt taagcaattt cttggcaaat tccagcgagc ccatggcagc
     2521 atacggttcc ggtattttcg cccagacata catcgaagcc ttcggcattt cgaccatcca
     2581 gcccgcttca tgcagccctt taaccaatac atcacggcgg cgtttgtact gttcggcaat
     2641 gtcgcgcacg cactgttgat cgccctccag cgccgcaatc gccgccacct gcaacggcgt
     2701 aaaggtgccg taatcgtgat agcttttaat acgtgccagg gcgctgacca gcgttttgtt
     2761 gccaaccata aagccgatac gccagcccgc catgttgtag cttttcgaca gcgtaaagaa
     2821 ctcgaccgcc acatcgcgtg cacccggcac ctgcatgatt gacggcgctt tccagccatc
     2881 gtagacgata tcggcatagg ccaggtcatg gaccaccagc acatcgtagc gcttcgccag
     2941 cgccacaacc ttttcgaaga actccagttc cacgcattgc gcggttgggt tcgacgggaa
     3001 gccgaggatc atcatcttcg gtttcggata actttcacga atggcgcgct ccagttcgtt
     3061 gaagaaatcg acaccttcca ccagcggcac tgagcgtacc tgagccccgg caatcaccgc
     3121 gccgtaaata tgaatggggt aacttggatt cggcaccagc actgtgtcac cgtgatccag
     3181 tgtcgccagc atcagatgcg ccaggccctc tttcgaacca atagtgacga tggcttctga
     3241 ttccgggtcg atttcaacgt cgtagcgatc ctgataccag cgggaaatgg cgcgacgtaa
     3301 ccgcggaatg ccacgtgaag tggagtaacc gtgcgtgtcc ggtcgctggg ccacagtaca
     3361 taatttttcg acgatatgcg gcggggtcgc accgtccggg ttacccatgc tgaaatcgat
     3421 aatatcttcg ccgcgccgac gcgcagccat tttcagttca gcggtaatgt taaaaacgta
     3481 gggcgggaga cgatcaatac gcgtaaaacg acgttcaggg cgagtgtcag ccataatttc
     3541 ctcagattaa cgtgagcgcc cggaccgtcc gagcgacgct gccacgatgg tggctgtttt
     3601 gaaaatagcc tgattaattt ctgtctgtcc agcgaattaa aaaatttttg ttgttaggtg
     3661 aaaaagggga acaccgcttt aaaaacagga aatttagcta cgccaacaaa aaatccccac
     3721 caattttatt aacattataa tttcaataaa tttaccaacc agacatgcat tatcaagaga
     3781 tgcattgcta atacaccatt tatctttcaa tcccctttta aaattgtggt ttttcctcaa
     3841 aagcagcaca ctggtcatcg gccttcaggt tacctatcat agaggtttaa tccttattca
     3901 gagtcacccg tgcacgaaat attcaacatg ctgctggcgg tcttcgatcg ggcagcgtta
     3961 atgcttatct gcctgttctt tctcatccgt atccgtctgt ttcgcgaact gttgcacaag
     4021 tcggcgcact ccccaaaaga gttgctcgcc gttaccgcca ttttctcgct gttcgccctg
     4081 ttcagcacct ggtccggcgt tcctgtagaa ggttcgctgg tgaacgtacg tattatcgcg
     4141 gtgatgtcag gcgggattct gtttggcccg tgggtgggca tcattaccgg cgtgattgcg
     4201 ggtattcacc gttatttaat tgatatcggc ggcgtgacgg cgatcccctg ctttatcacc
     4261 agcattctgg cgggttgtat atcgggctgg atcaacctga aaatccccaa agcgcagcgc
     4321 tggcgcgtcg gtattctcgg cggcatgttg tgtgaaacgt tgaccatgat tctggtgatt
     4381 gtctgggcac cgaccacaga gcttgggatc gatatcgtct ctaaaatcgg cattccgatg
     4441 atcctcggca gcgtctgtat cggctttatt gtgctgctgg tgcaaagcgt tgagggcgaa
     4501 aaagaggcca gcgccgcgcg gcaggccaag ttggcgctgg atatcgccaa caaaacgcta
     4561 cctctgtttc gccatgtcaa tagcgagtca ttacgcaaag tttgtgaaat tattcgcgat
     4621 gacattcacg ccgatgcggt ggcgattacc aataccgatc atgtgctggc ctatgttggc
     4681 gtgggtgaac ataactatca gaatggcgat gacttcatta gcccgactac ccgtcaggcg
     4741 atgaattacg gaaaaatcat cattaaaaac aatgatgaag cccaccgcac accagagatt
     4801 cattccatgc tggtgatccc attgtgggag aaaggcgttg taaccggaac gctgaaaatt
     4861 tactactgcc acgcgcatca gataacctcg tcgctacagg aaatggcggt cggtctgtcg
     4921 caaatcatct ccacacaact ggaggtttca cgtgccgagc agctacgtga aatggcaaat
     4981 aaggcagagc ttcgcgccct gcaaagcaaa attaatcccc attttctgtt taacgctctg
     5041 aacgctattt catcgtcgat ccggctgaat ccggataccg ctcgccagct gatctttaat
     5101 ttgtcgcgct atctgcgcta taacattgaa ttaaaagacg atgagcaaat cgatatcaaa
     5161 aaagagctgt atcaaattaa agactatatt gccattgaac aggcccgctt tggcgacaag
     5221 ctgacggtta tctatgatat tgatgaagag gtgaattgct gcattcccag cctgctgatc
     5281 cagccgttgg tggagaatgc cattgtccac ggtatccagc cttgcaaagg taaaggcgtt
     5341 gtcaccatca gcgttgcaga gtgcggcaat cgggtacgca ttgcggtgcg ggataccggg
     5401 cacggcatcg atccaaaggt gattgagcgg gtcgaagcga atgaaatgcc gggcaataaa
     5461 attggcctgc tgaatgttca tcatcgcgtg aagttattgt atggcgaggg gctgcatatc
     5521 cgccgcctgg aaccaggaac ggaaattgcg ttttacattc ctaaccaacg caccccagtc
     5581 gcctcacagg ctacgttatt gctttgagcc ggagtgatat tgtgaaagtc atcattgttg
     5641 aagacgaatt cctggcacaa caggaactga gctggctaat aaaagagcac agccagatgg
     5701 agattgtcgg cacctttgac gacggtctgg acgtgttgaa gtttttgcag cataaccgcg
     5761 tcgacgccat ttttctggat atcaatattc cgtcgctgga tggtgtgttg ctggcgcaaa
     5821 acatcagcca gttcgcccat aaaccgttta ttgtgttcat caccgcgtgg aaagaacatg
     5881 cggtagaagc gtttgaactg gaggcgtttg actacattct caaaccgtac caggagtcac
     5941 gcatcaccgg aatgctgcaa aaactggaag cggcctggca gcaacagcag accagcagta
     6001 cgcctgccgc aacggtaacg cgtgagaatg acaccattaa tctggtgaaa gatgagcgaa
     6061 taatcgtcac acccattaac gatatctatt acgccgaagc gcacgagaaa atgacctttg
     6121 tctatacgcg gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt tgcagcaaac
     6181 tgccgccgtc gcattttttc cgctgccatc gctcattttg tgtcaatctg aacaaaatcc
     6241 gcgagatcga accgtggttt aataacacct acattctgcg actgaaagat ctggattttg
     6301 aagtgccggt cagccgcagc aaagttaaag aattccgcca gttaatgcat ctttaatcgg
     6361 gaactttcat gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc gatctgttca
     6421 gcggcctggt gcttaacccg caactactgg ggcgcgtctg ttttgccagc cagcctgcct
     6481 cgttgccggt gggcagttta tgtattgatt ttccccgtct ggatatcgtg ctgcgcggcg
     6541 aatacggcaa tctgctggaa gcaaagcagc aacgtttggt ggaaggagaa atgctgttta
     6601 ttccggcgcg cgcggctaat ttaccgatca acaacaaacc ggtgatgctg ttaagtctgg
     6661 tgttcgcacc gacctggctt gggttatcgt tttacgatag ccgcaccacg tcgttgttgc
     6721 atccagcccg ccagatccag cttcccagcc tgcaacgcgg tgaaggtgaa gcgatgctta
     6781 ccgccctcac ccatcttagc cgttcgccgc tggagcagaa tatcattcag ccactggtgt
     6841 taagtttgct gcatctttgc cgcaacgtgg tgaatatgcc accgggcaat tcgcagccgc
     6901 gcggcgattt tctctatcac agcatttgta actgggttca ggataattat gcccagcagc
     6961 tcacccgcga gagcgtggca cagtttttta atatcacgcc caatcatctg tcaaaactgt
     7021 ttgctcagca tggaacaatg cgttttatcg aatatgtgcg ttgggtgcga atggcgaagg
     7081 cgaggatgat tttgcagaaa tatcatctgt caattcatga agtggcacag cgttgcggtt
     7141 ttccggatag cgactatttt tgtcgcgttt tccggcgtca gtttggtctg acgccgggag
     7201 agtacagcgc ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc agcagcgtca
     7261 ggatactttg tgcatcttgc gcggcaaaca acgactggcg gaagttttta ttcaccagtt
     7321 tacgcgccag ctgcgagaag actttcacat gattcatccc ttcgttagca cccagcgtca
     7381 gcatgatcac cagttcgact tcgcccattt ctgactgcca gtcaaccggt ttcgccagcc
     7441 gggcaatgct gatactggaa tgacggatcc actgagattt ggtgtgcggg atcgccacgc
     7501 caaaaccaac gccagtggtg acaatctctt cccgctgcca gacatcctct tccagctcga
     7561 acggatgttc agtgcgcccg ttaacgccga ggttaccgca caggaactgg atcgcctgct
     7621 ctttattgct aaaggcctga tcaacgaaga tattctccag cgccagtaat gggcgaacgt
     7681 cttcttccgg cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcgctg cggcattcac
     7741 atgcctgacg cgccagttcc cgacacgcct cgctatccag ttgacgaagc tggcttttca
     7801 ccgccggaat gcgcgggcta ctcatactca gctcatccag gcccagccca agcagtagcg
     7861 gcagataacg gctttcaccg cccagttcac cgcaaatgcc tacccatttg cctcgctgat
     7921 gcgcagtggt aactatctgc tgcaacatgc gcaggaacga tggcgtaatc gggttataca
     7981 gcggcgatac gcgcgggtta ttacgatcga ccgcatacag atactgggtc atatcgttgg
     8041 agccgatact gaagaaatcg acctcatcgc agaagtggtc gatgatgtag cacaccgacg
     8101 gcacttccac catgataccc agcgtaatcg tctctgcatg acgcaggcca tcgcgcttaa
     8161 gctcaacgat cgctttttgg atctcgccct tcacccataa gatctgatcg aggctgtgaa
     8221 ccatcgggat catcaactgg gcgttgccga aactggcggc gcgcaaaatg gcccgcagtt
     8281 gagtgcggaa cagaccagca aattccgggt aaatacgtac cgcgcgatag ccaaggaacg
     8341 ggttctcttc ctggggaata ttcagataag gaatgctttt atcgcctccg atgtccattg
     8401 tgcggaagat aatcggcttg tcgcccgctg ccagtagcac ctgctggtag gcttcaaatt
     8461 gctcctgctc gtccggcgcg ctgtcgcgat ccatatagag catttcggta cggaacagcc
     8521 cgacgccttc cgcgccgttt gcaaacgcgc caggcgcttc cagagcggtg ccaatattcg
     8581 ccgcgatatc aatacgcttg ttatcacggg aataagccag ctgcgcggca gcctgcgcct
     8641 gttgtttttg gcgtttatcc gccagcgtct gcgcgacctg ataataaccg ctcaccgcgt
     8701 cattcgggtt aatcgccagc acgccgcact gggcgtcaag cactgcaggt tgcccggcat
     8761 aacgggcaat cgcatccagc ggcaggccgc tcagtaccgg gatcgccgag gcgcgggcca
     8821 gaatcagggt atgcgaggtg cgcccggttt tctccagaat catgcccgca agatttttta
     8881 aatccaggct taaaaactgg cttggggtta aatcctcggc caccagaata gtcggttttt
     8941 ccagcaccag attgttgcgc ggcttcagtt ccggccaggt aatatgcagc aactgttcgc
     9001 taatatcgcg aatgtcgcta acacgttcgc gcagataatc gctggcagaa gcagaaagtt
     9061 tggcgcaaac ctgctccata ttgctgatga tcgccgcccc cagcccctga tgctgttctg
     9121 tcatcaggcg acggatattg cctgcaaatt catcatcctg aatcagcgac aaatgggcgc
     9181 tgaggatagt tttgctttcg ccgtcacgct cacgcaattg ctggttcagt tgctcggcaa
     9241 gcgttgccag gctgtgctcc agccgggtgg aatcttgcgc actggcgggg attgcccgat
     9301 aactgtcgag gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg ccgcttgcca
     9361 gcacattgcc gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc agcggatgcg
     9421 ccgtcagttc tgcctgcgta ggctgaacgc tgtcgctgtc gataaagcgc acctggatgt
     9481 actcttccag cacgcgccgc gcctgctctt catcgctgcc gctaatgttc aggctgcaac
     9541 tgtcattaaa tagggtgccg gtgccaatca gcgccagcga gcttttggca tcggcctttg
     9601 cgttctggcg atggttaata aaagtgattt cgctttgcca ctggctgcac tgttctttaa
     9661 gttcccacgc cggacgagca tgtagaccgt taggcaaagg acagagaaat tgaatcgtta
     9721 acataaccgc tccgggttaa tcaggagatc atctgaaatc cgtcagttga acaaccgtct
     9781 cacgcgtaag acgttgaata agggcagata aaagttgctc catctgcaaa atgtcgcggc
     9841 aatcggcaat cgatgcggcg caatgtccgt ggcgggtggc aggccccatc accactgtgg
     9901 gtacgccggt accggttaag tgcaccactc caccgtcagt gccgccgtta ctgaacatat
     9961 ccgcctgtaa cggcacgcca atttctgccg ccacggtttc gatccaggcg gtgagttttg
    10021 gcggcgcaat cagtgacttg tcgcttaaca ccagcatcgg gccgttacca atctggcgat
    10081 ggttagccgc gccataatca aagtttttcg cccagcaggc ggtatcaagg acaatggcga
    10141 cgtccggcga caccgcgcgg gtggcagttt gcccgccgcg taatcccacc tcttcgctgg
    10201 aacttgccac cagccacact tccgcaggca gttcggcgtc gtgcagctca cgtagtaacg
    10261 tcaccagcag atagcaaccg aggcggtcat caaaggcttt ccccatcacc cgctggtggg
    10321 ggagaacctg aaaagtggta tcgaacgtga cgcgatcgcc tggacgaatt cccgcctgca
    10381 tcacttcgtc atacgagcgc gcgccaatat ccacgcgcat ggcgctgaca tcattcccct
    10441 gccggtcgcc gtcaagcagg cctggaattt tgcactcttc acgggtggtg atgcgcaccg
    10501 gctgcagctg gcgggcagcc atgcgtacat tgccaaccgg cagcacatca atcgcccctt
    10561 cgcgggagat gctgcgcacc ataaatccca cttcgtccat atgcgcacag atcatcacct
    10621 tcggacctgt cgattcattc aggcggatca gcaccgatcc cagaccatca aatcgcactt
    10681 ctttttgcag gcgatccgct tcttccagca ggatctgccg cacttcctgt tccgaggagg
    10741 cgatcgcatc tgcctcgctc aacgctttta atagcgataa atccattatg cctctcccgt
    10801 gagcaacact gttttcggca tggcgtagag cacttctgcg ccttgcgggg tgaccagcac
    10861 aacatcctcg atgcgcacgc ccccttgccc tggcaaataa atccccggct ccacggtcag
    10921 taacatgcct ggctgtagcg tcgtggtatc ccgcggtgaa aagcgcggat cttcgtgtac
    10981 ttcaatgcca atagcatgac cggtgttgtg accaaaatag tcgccaaatc ctgcctcggt
    11041 aatcacccga cgcgcggctt cgtcaacctg ctggcagcgc acaccggggc gaattgcaga
    11101 gattgctgcg agctgtgcct gcaaaacaat ctgatagacg ttaaacagcg gatgagattc
    11161 ggcgctcacc ccttcgccat tcaccagcaa ggtgcgcgtc atatcagagc agtagccctg
    11221 atacagcgca ccgaaatcga gagtgacaaa ctcgcccgct gcaacaatct tgtcgctggc
    11281 tttgccgtgc ggcagcgccc cacgccagcc actggcaaca atggtgtcaa aagaggtttt
    11341 ttctgcgccc tgctggcgca taaaccactc cagttcagcg gctatctcgc gctcgctcat
    11401 ccccgcctga ataaagcggc gaatatgctc tgcaccgcga tcggcaatcc cacaggcaag
    11461 gcggattttc tccacctcct ctggcgtttt gatttgccgc agcacatccg gcgtggcgct
    11521 aaccagtttc gcattgagtt cagactgcca gcggtgcgcg gtttcccaac tcacctgctg
    11581 gccttcaaaa ccgagcgttt gcaactgttc atcggcaatg atttcattga cgatggttgc
    11641 aagcgtgtgt gtcgcgtcaa gcaaatgcag ctggtaacct tgcgtacgag cttctacatc
    11701 cgcgtaatag cgcgagtcca ccagaatgtg ctcactttca cggctaatca gcacatagcc
    11761 tgatccggtg gagatcccca gatgcggctg tttgttctgc cgtgaggaga gaagcactgc
    11821 atccagttgt tgcgccttaa gccagtcgcg cagcgaagcg agtaatgtca tctggatttt
    11881 ccttcttaca ggctatcgat taacaatttg cctttgcgga acatcatcag acgcaggaac
    11941 accaccatca gcgcagtaat gattgccccc agcgcgatcc ccgccatata gacgcccaag
    12001 ttagtgacca gcggccatgc ccagatagcg gactccggga accattgcac tgcgcccagc
    12061 cagacagctg cggttgagcc gacaatcgcg ccaaccatat acgacggaat ggcggtgatg
    12121 gggctttcca gtgcaaacgg aatcgcaccc tcactaatcc ccataaacgc caggaacatc
    12181 gcggttttac cctgcggata aagctgagca ctgaacagac gtttgccggt taaacggcgg
    12241 tcgataatgg tcgccagacc cagaccaatc ggcggaataa caatagcgat agaacgagcg
    12301 gtgaccggta atacgtggtc agtggtaaag ctgaaggcaa cgaaacctgc ggctttgttg
    12361 atcgggccac caaggtcgat tgctgtcgcc gcggcgatcc ccattgcgta catcagcgcg
    12421 cccttctcac ctgcggcagt cagcacagta cggataccgc cgttgatcca gccaccaaat
    12481 ggtgtgatga cgtagtacat cgccagcatg acaaaaatag cggaaagaat cggtagcaaa
    12541 aatgtggttt tgaacgctaa caggaaatcc ggtaactgga ttttctggtt catccatttc
    12601 acaaggtaac cagcaacgat agaaataatc agcgcaccaa taaaggtgga cggcaccggc
    12661 gaagaggtcg cccattgcat agtgctggga tcgaagttca gcagttgcgt cggctgggta
    12721 gacatcaagc cgccgataaa accagccgga aacgccagtt tgccaccgat agagttcgcc
    12781 acaaaagcag caaacatcgg aatggcaaaa ccaaacaaca cgccgccgaa ggactgcgac
    12841 aaccaggcaa atttcagtaa ggagaggtcg aaaccggaga atttcccgct attaagagca
    12901 tccatgatgc cgatatcagc aggaatttta agccagctat aagcaatcaa ctggctgaaa
    12961 gcgaggatca caccgcccat aattaaggtt ggcaccatgc gtgaaatccc tgacatcaca
    13021 tgctgcggga gttcacccca gaagctgctt ttagaggcct gcggattctt aactgccgct
    13081 gccgcaccgg atgcgccagg cacaacggtt gcactgcgtt ttttaatggc cataacaatt
    13141 atccctgtcg aatattactg ttgttcagag gcaatcatct cttcgatttc tttgatgatg
    13201 cccgcagcgt ttttaattgc gtcctgcaaa gtgatttcat aaacgtcgcg tgattcgaaa
    13261 cgttcgttat cttccggggt aactgccacg gaatggatga tgatggtcgc ttcggcgata
    13321 tcctgcgccg tcaggcgatt ctggatgccg tccgcgccct gggtttcaat tttcacttca
    13381 taaccggctt ctaccaccgc ttcttccagc gcctgagcgg ccataaaagt gtgagccagg
    13441 cccattgggc aggcacataa ggcaatcagt ttcttactca tcgttaattc ctcgttcagt
    13501 gatagttgac gagtgaaatt atgaacgtgc aaaacaaatc gccgttacca gacaaataat
    13561 gcatttactg gagaattaat cccgctgtac ggaagtgtga cgcaggtcgg ccttgtgagg
    13621 ggacacaaag aaaaattgaa ttaactgtat gatttaaaag attatcggga gagctacctc
    13681 ccgatataac aggaaggatt tacagaatgt gacctaaggt ctggcgtaaa tgtgcaccgg
    13741 aaccgagaag acccggattg tcatggacga tgagatacac cggaatatca tggacatatt
    13801 ctttaaagcg ccctttatct tcaaatgcgg cacggaaacc agaggctttg aagaactcaa
    13861 ggaagcgcgg cacgataccg cccgcaataa acacgccgcc aaatgtcccg agattgagcg
    13921 ccagattgcc gccaaaacgg cccataatga cgcaaaacag cgacaacgca cggcggcaat
    13981 cggtgcagct gtcagccagc gcgcgttcag taatatcttt tggcttgaga ttttctggca
    14041 ggcggttgtc agctttcaca attgcgcgat acaaattcac cagccctggg ccagaaagca
    14101 cgcgttccgc cgaaacatga ccaatttccg cacgcaatat ttcgaggata atggcctctt
    14161 cttcactatt cggcgcaaaa tcaacgtgac cgccttcgcc cggcaagctt acccaacgct
    14221 tatcgacatg gaccagatgc gcaaccccaa gccccgttcc ggcaccgtaa accgcaatag
    14281 gcttaccttc tatcggctct gcgccaccaa actgaatcag atgctctttt ttcagcatcg
    14341 ggatcgccat cgatacagcg gtaaaatcgt taataatttc cagatggcta aaaccgagat
    14401 tctttttcat ttcggcaatt gagaacgccc aggtatggtt ggtcattgcc acccagtctc
    14461 cggtaatcgg gcaagcgatg gcaatacagc cgtctttcac ctcgacctta tgttcttcaa
    14521 gataaacgcg aatgaccgct tcgaggctgg ggtaatcaag ccctgaatag gtcttagcct
    14581 gcgagatttc accactggca atatcacaca gagcaagacg tgcgttggtg ccgcccacat
    14641 caccgactaa tgcatacttt gtcattcttc aactgctccg ctaaagtcaa aataattctt
    14701 tctcacactg taaatacctg ggggcataac aacaacgccg gaaaggcagg ctccctgtaa
    14761 atatcgatct gggtcacaca attactttat cgtttcagca ccaattgcag cgatgccttt
    14821 ttgcaagctg ggcaaactaa gtatctgacc ccgcataagg aatagaacat gctccatccg
    14881 cgagccagaa ccatgttgtt attatcgctc cccgccgtgg caattgggat tgcgtccagt
    14941 cttattctga ttgtggtgat gaaaatcgcc tcggtattac agaatttgct ctggcaacga
    15001 ctgccgggaa ctctggggat agcccaggat tcacccctct ggatcatcgg tgtattaacg
    15061 ctaacgggta ttgcggtggg gttggttatc cgtttcagcc agggtcatgc cggaccagac
    15121 cccgcctgtg aaccgctgat cggcgcaccg gttccgccct ctgcgctacc aggacttatc
    15181 gtagcattaa ttctcggtct tgctggcggc gtcagcctgg ggccggaaca tccgatcatg
    15241 accgtcaata tcgcccttgc ggttgccatt ggcgctcgtc tgttaccgcg cgtcaaccga
    15301 atggagtgga ctattttagc ctctgccgga accatcggcg cgctgtttgg tacacctgtt
    15361 gcggcggcgt tgatattttc gcaaacctta aatggcagta gtgaagttcc gctatgggat
    15421 cgtctttttg cgccgttaat ggcggcagca gctggtgcac ttactaccgg attatttttc
    15481 catccccatt tttcactgcc cattgcgcat tacggacaga tggaaatgac cgatattctc
    15541 agcggtgcaa ttgtcgcggc gattgccatc gcagcaggga tggtcgccgt atggtgctta
    15601 ccacggttgc acgcgatgat gcatcaaatg aaaaatccgg tgctcgtgct gggtattggc
    15661 ggatttattc tcggtattct gggggttatt ggtggaccag tttcgctgtt taaagggctg
    15721 gatgagatgc agcagatggt ggcaaatcag gctttcagca ccagcgatta ctttttgctg
    15781 gcggtaatta aacttgccgc cctggtcgtt gctgccgcca gtggctttcg cggtgggcga
    15841 atcttcccgg cagtgtttgt cggcgtggca ttagggttga tgctgcatga gcacgttccc
    15901 gccgtaccag cggcaataac cgtttcctgc gctattctcg gcatcgtgct ggtggtaaca
    15961 cgcgatggct ggttaagtct ttttatggcg gcagtcgttg tacccaatac cacattgcta
    16021 ccgctgctct gtatcgtcat gcttccggca tggctgttat tagcaggtaa gccgatgatg
    16081 atggtcaatc gtccgaagca acagccaccc cacgataacg tttagcaaaa aatgctctcc
    16141 tttatgattt aagagttatg gcgatgatac ggggctttac ctccccgtaa tattgcgtta
    16201 acaggccgct gacggcttcg atcgtgaagg agaataacaa tgttcaggtc actgtttctg
    16261 gcggccgccc tgatggcatt taccccgctt gcggcaaacg caggcgaaat caccctactg
    16321 ccatcaatca aattacaaat tggcgatcgc gatcattacg gtaattactg ggacggtggt
    16381 cactggcgcg accgtgacta ctggcatcgc aattatgaat ggcgtaaaaa ccgctggtgg
    16441 cgtcatgata atggctacca tcgtggctgg gataagcgta aagcatatga gcgtggctat
    16501 cgtgaaggct ggcgcgatcg tgacgatcat cgcggaaaag gccgcggaca tggacaccgc
    16561 cattaaacac attcgccaat ggagcacaat gcctgatgcg acgctagcgc gtcttatcag
    16621 gcatacaaac cggctcttta acgcgtaggg cggataaggc gtttacgccg catccgccaa
    16681 tagtgccaga tgcgacgctc attcaactac aatcccagcg ccgtccccac caacaaccag
    16741 atattcagcg ccacgaccag cactacaatc acccagcctg tctgttttac acgtttgctg
    16801 ttcaccagat cgcccatcaa cttgctgtca ctggtgaaaa tcagcagtgg aaccagcgcc
    16861 agggcgatac caaaacttaa cagtacctga ctcataacca gaatccgtgt cggatctaat
    16921 cccatcagaa tgacaataaa tgacggcagc atggtgactg tacgacgcac ccacagcggg
    16981 atatgaaagc gaatgaagcc ctgcatcacc tgccccgcca gtgtccccac caccgttgag
    17041 gacagtccgg cagcaaccag gcttaatcca aagaccgttg ccgcagcgtg gcttaatagc
    17101 ggttgcagcg tcagataagc ctcatcaaga tcggcaacac cagtatgacc ggaaaagtgg
    17161 aacgccgccg cagctgtagc catcatcgcc agattgacaa aaccggcaat agtcatggcg
    17221 attgccacat cccatttggt ggcggaataa cgttgttgac gcgaaccgcc atgtaaatgt
    17281 tgagtgagcg aagagtgcaa ataaatcaca tgcggcataa tcgtcgcccc taacacgcct
    17341 gctgccagga agaccgcctc cgaagtaggt aaactcggga tcaccattcc tttacccagc
    17401 tgcgccaggt taggctggga gaaaatcaac tcgacaatgt aagccgcggc aacaaacaac
    17461 agtaacccgc caatcacttt ctccagcggt ttttgcccgc gacgttgcag cattaaaatc
    17521 aggaaagtcg cgatccccgt cagcaccgcg ccctgcaaca acgaaacacc aagaatgagt
    17581 ttaaaaccga tcgccgcacc aataaattcc gccaggtcgg ttgccatcgc aataatttct
    17641 gcctgaaccc aatagaacca cactacggga cgcggatagt gatcgcgaat ctgctccgcc
    17701 agatttttac cggtggcaat ccctagtttg gcagagagga tctgaatcag catcgccatc
    17761 aggttggccc aaacgacaac ccacagtagc tgatagccga agctggcacc cgcctgaata
    17821 ttggtcgcaa agttaccggg atcgatataa ccaatcgccg caatgaacgc aggtcccatt
    17881 aatgcgagcc tcatcttgcg cgccgcccgt ccgctgctac tctcaacgcg atagttcgtc
    17941 atcttgtgcc tctaaaacat agcctttgct atgtttcatg ctatgccaaa cgagaatgat
    18001 tatcaaattc atttaaatgg gttgtggtga tttctctgat agaccaggat tatgactacg
    18061 aaaagattga tgacttgaat gttatggtta tgtttaatgt tagcacattt acataacttt
    18121 cagcttccat acacaacata gcagaaatgt atgacagctc actatttttg aagcttgtca
    18181 caggacgtca ttatagtgtg tgtcagatct cgttttcctt aaccatgtta catagaatgt
    18241 gcacggaaat ttaacctgcc tcatatttgg agcaaatatg gaccgcgtcc ttcattttgt
    18301 actggcactt gccgttgttg cgattctcgc actgctggta agcagcgacc gcaaaaaaat
    18361 tcgtatccgt tatgttattc aactgcttgt tatcgaagtg ttactggcgt ggttcttcct
    18421 gaactccgac gttggtttag gcttcgtgaa aggcttctcc gaaatgttcg aaaaactgct
    18481 cggatttgcc aacgaaggga ctaacttcgt ctttggtagc atgaatgatc aaggcctggc
    18541 attcttcttc ctgaaagtgc tgtgcccaat cgtctttatc tctgcactga tcggtattct
    18601 ccagcacatt cgcgtgttgc cggtgattat ccgcgcaatt ggtttcctgc tctccaaagt
    18661 caacggcatg ggcaaactgg aatcctttaa cgccgtcagc tccctgattc tgggtcagtc
    18721 tgaaaacttt attgcctata aagatatcct cggcaaaatc tcccgcaatc gtatgtacac
    18781 catggcagca acggcgatgt ccaccgtatc tatgtccatc gttggtgcat acatgaccat
    18841 gctggagccg aaatatgtcg ttgcggcgct ggtactgaac atgttcagca cctttatcgt
    18901 gctgtcgctg atcaaccctt accgtgttga tgccagtgaa gaaaacattc agatgtccaa
    18961 cctgcacgaa ggtcagagct tcttcgaaat gctgggtgaa tacattctgg caggtttcaa
    19021 agttgccatt atcgttgccg caatgctgat tggctttatc gccctgatcg ccgcgctgaa
    19081 cgcactgttt gccaccgtga ctggctggtt tggctacagc atctccttcc agggcatcct
    19141 gggctacatc ttctatccga ttgcatgggt gatgggtgtt ccgtccagcg aagcactgca
    19201 agtgggcagt atcatggcga ccaaactggt ttctaacgag ttcgttgcga tgatggatct
    19261 gcagaaaatt gcttccacgc tctctccgcg tgctgaaggc atcatctctg tgttcctggt
    19321 ttccttcgct aacttctctt caatcggaat tattgcaggt gcagttaaag gcctgaatga
    19381 agagcaaggt aatgtggttt ctcgcttcgg tctgaaactg gtttacggct ctaccctggt
    19441 gagtgtgctg tctgcgtcaa tcgcagcact ggtgctgtaa gaccatacat aaaaaagccg
    19501 gggataattc cccggctttt ttacgcctgt taatcagcta atggctgcgg gcgaccaatc
    19561 aaataccctt gcagatattg caccccgagc ttatgcaata gcgcctgttg ctgttgcgtc
    19621 tcgacaaact ccgcgaccac actcaatgac ttcgctttcg ccagatcggt aattgatctc
    19681 acaatcatcg catccagcgt gttcgtgacg atatctttca caaagacgcc atcaattttg
    19741 atgatatcag cctgcaaacg ctttaaccgt tcgtagttgg catagccggt gccaaagtca
    19801 tcaatcgcaa tccggaaacc aaacttatgc agctgctcga tgttgtacat gctgctttct
    19861 gcgttagaaa acgcctgctc ctcggtgatc tcaagaatga ccgcctgcgg agagatgtga
    19921 taacgcttaa atagacgaat aatccgcccg gcaatattct tttgcagcag cgtgagcggc
    19981 attaaattga ctgaaaagcg tgggcctttt ttgtcgcaag ggtgtgttgc cagccacttc
    20041 aacaaggatt ccagcacttg caaatcaaaa cgcgcgctaa ggttaaactg agcaataagg
    20101 ggcagaaact tatccggggt cataatgccg ccgtcatatt tcagtcgcgc gaggatctca
    20161 tcataacctt caccctcttt gttgcgaatt ggctgggcgt agagcagcaa atcaccctga
    20221 tctaacgccg tgcgaatggt attcagcaat agcacctgtt tagtggtctg cccggaaacc
    20281 atctcctctc ggctatccag cgccagcaca tgatgatgtg cgcaggattg ctccgcgagc
    20341 cagcttaact gccccaacaa gggttgcaag gtttcctgat ttccatcaaa acgcccccag
    20401 gcagcaccat agcccatatc cagcccggta ttgttccagt gaatttgccg actattcagg
    20461 atgttaacca tatgctggag tcgcccttcc gtttccggcc cgctcagcac taacagcagt
    20521 tcactacccg gcaattgata caacttttcg ttttcctgca tcaacggcag cagcgtacgg
    20581 taaattgatc ggatacagtg aacgcgcatc attaacccgt aatgacgact cataaactca
    20641 agattatcaa tgcgcaggca gcaaaaactc ttgcccgcct cttgctccgg cgcttgttcc
    20701 aacgcacgaa agttgggcag gagcgttaac ggatcggtca gcgcctgcaa atgccagcgg
    20761 cgattaagcc attcactgcg atgataaatg cgcaccatat agagcaggca aacgctaaag
    20821 gaaatcagca ccgcaagaat aaatgccagc gaatattcgg tttccacccc ttgcaaaaag
    20881 ttctggttgt aattcagcag gcaaagcgtt gaaacagccc aagtgagatt taaaaacgga
    20941 tagcgaagct taccgacccc gagggtaaag atgatgaaga aaacgggtac caggtaaccg
    21001 gcaataaagt cgttttcata aggtgtgcac aacagaagta gcagcacgct taacgctgcc
    21061 agccagctta aggtaaatgc gcgtttctct ttgcccaacg acggagcgat atccctgcgc
    21121 cacaatatct gcgcaaagtg gggacttaca atcatgcgag tgagatagta gaaaagcatg
    21181 ttgtaaatca gcaccgcggt gaaaaggctt agcaaatcaa cgaccgtgaa aatggcatcc
    21241 gcatcgccga aaaaggtgga tatctttagc gggaaatcaa agaagctccc cacaagatac
    21301 atgctgcatt tgatgccgat cggcgtcacc aaaccaagcc agaacagacg ttgccagaca
    21361 tatcggctgg tcaatccata acgccaacgc gtccccagct gccagcgtaa tattgcgcag
    21421 gcacataaca cgacaaacgt ctggctaccg agcatgaccg cagtctgtaa aaaagagaga
    21481 tgaaagttcc acagattggt gcaaaacatc ccgcataaaa cgggaacgac accgcgccag
    21541 ccaaagataa acaagattga cagcattacg cacagtggca tccacgccag aaagatataa
    21601 ctggaatgga taatggcaag tggcgaaata aaacgggata gctgaataag taccacggtg
    21661 agcgtaaacg ctagtgtgaa tatcttgata ttttttatca ggttatgctc cacaaacatg
    21721 agtacactac tctcactgat cggggttttc aggcacacaa ataataagtt tattcgcatt
    21781 gcgaagcaaa caggggccat cctgttgccg atttttcatc caatgatgat ttcaccaggc
    21841 gacactaacc cggacgtaga ctgtattaca aaagcggcaa aaagcagaga caaaaaaccc
    21901 ccgctttgca gcgagggttg gaaatttggt ggagctaagc gggatcgaac cgctgacctc
    21961 ttgcatgcca tgcaagcgct ctcccagctg agctatagcc ccacgatgcg tttacgtacc
    22021 aaatttgctg ggtgcaaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    22081 tgccatgcaa gcgctctccc agctgagcta tagccccgtc acgtaaagct tgtcgagttg
    22141 acgggcggca tcatatgaat tccgcccgaa tgtgtcaacg gcaaattgca acgtgtagtt
    22201 tcaatcgctg aaaaatcagg caaatgaaca attttggaaa accgctcgca ttcagtagtt
    22261 attcatgtca cggtttcctg taaagtggtg ctataaaatg aactactaat agacccacat
    22321 acattcaggg aattgttatg ttcaaggaga ggatgacgcc agatgaactt gccagactga
    22381 ccggctatag ccgccagacc attaataaat gggtacgcaa ggaaggctgg acgacgtcac
    22441 caaaaccagg cgtccagggt ggcaaagccc gactggttca cgtcaatgaa caggttcgtg
    22501 aatatattcg taatgctgaa cgtccagaag gtcagggaga agcgcctgcc ctttctggtg
    22561 atgcgccgct tgaagtattg ctggtgacac tggcgaaaga gatgacgcca gttgaacaaa
    22621 aacagtttac atccttgctt ctgcgggaag ggattatcgg attgttacaa cgcttaggga
    22681 ttcgcgatag caaataatat gaaaagatta cgcaataaaa tgaccaccga agaactggct
    22741 gaatgcctcg gtgtggctaa acaaactgtt aaccgttgga tcagagaaaa aggctggaaa
    22801 acggaaaaat ttcccggcgt gaaaggcggt cgtgccagac ttattctggt cgatacgcaa
    22861 gtttgcgagt ttattcagaa cacgcctgcg ttccataaca caccaatgtt gatggaagct
    22921 gaagagagaa ttgctgaata cgctcctgga gcacgcgccc cggcgtatcg acaaatcatc
    22981 aacgcgatag ataatatgac agacattgag caggaaaaag tcgcgcaatt tttgtcacgt
    23041 gaaggaattc gcaatttcct cgcccgtctc gatattgacg aatcagcata aataaaaaac
    23101 ggcaggatat tatctcctgc cgtttatctt tttacacgct aattactgct ggttttcgcg
    23161 ttcagcaata aaggccagcg ctttgttgat acgctcaatg ctacgtgtct taccgattgc
    23221 gtgaacggta acatccagtg ccggagactg ccccgcaccg gttacggcga cacgcagcgg
    23281 cataccaact ttacccatac ccacttccag ctcatccgcc gtcgcctgaa tagcgtgatg
    23341 aacgttttca gctgtccagt cagtaatcgc ggccagtttg tcacgaacga cttccagcgg
    23401 ctgacgcgct accggacgca gatgtttttt cgcggcgtcg gcatcgaact cagcaaaatc
    23461 ttcgtagaaa taacggcagc tctgtgccat ctctttcagc gtcttgcagc gttcgcccag
    23521 cagtttcacc agatcagcca gctgcgggcc gttacgggta tcgatatttt cctgctcaat
    23581 gtgccactgt aagtgagtag caacatactc cggcggcagc gcgttaatgt agtgatggtt
    23641 cagccacagc agcttgtcgg tgttgaacgc actggcagat ttgctgacgg cattcaaagt
    23701 gaagtatttg atcatctctt cacgagtgaa gatttcctga tcgccgtggg accagcccag
    23761 acgcaccaga tagttcagca gtgcttctgg caaataaccg tcatcacgat actgcattac
    23821 gctgactgcc ccgtgacgtt tggacagttt tttgccgtca tcgccgttga tcatagaaac
    23881 gtgcgcgtaa accggtaccg gcgctttcag ggctttaaga atgttgatct ggcgtggcgt
    23941 gttgttgata tggtcttcgc cacggataac gtgggtgatt tccatatccc agtcatcgac
    24001 aaccacacag aagttatagg ttggggaacc atcggtacgg cggataataa gatcgtccag
    24061 ctcctggttg ctgaactcga tcggaccacg gatctgatcg tcaaaaacaa cagaaccttc
    24121 ctgcgggtta gcaaaacgta cgacacacgg ctcatcatca gcgtgatgct catgactgtg
    24181 gcggcagcga ccgtcataac gcggcttctc acctttcgcc atttgttctt cgcgcagcgc
    24241 ttccaggcgc tctttagagc aatagcattt ataagcagtg ccctcctcca gcatctgatc
    24301 gatcactgcg ttgtagcgat caaaacgttt ggtctggtag tacggacctt catcccactc
    24361 caggctcagc cagttcatgc catccataat ggcttcgata gcttccggcg tggaacgctc
    24421 aagatcggtg tcttcaatac gcagcacgaa ctcaccgccg tggttacgtg caaaaagcca
    24481 ggagtaaaga gcagtacgcg cgccgccaac gtgcagatag cctgttgggc ttggcgcgaa
    24541 gcgagttttg attttcatga aatggcctta cgtttagaaa gatgccgaca accggcaaat
    24601 cctggaaaaa ttgagtgggc gatattctat cactcccgcc tgattcctca atgtagttcg
    24661 ggtttctacc ttacgcatta ttgttttcgt tgacaaattg cgcaacacgg tctgttttgc
    24721 gatcatcttg cttaaattta cgacgaacga acaatttctt tagaaaatgc gttgactcat
    24781 tttgaactct ccctataatg cgactccaca cagcgggggt gattagctca gctgggagag
    24841 cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac caactacttt
    24901 atgtagtctc cgccgtgtag caagaaattg agaagtgggt gattagctca gctgggagag
    24961 cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac cactttctcg
    25021 ccagctaaat ttcttgtaac aatgtgaagt accgaagtgg gtgattagct cagctgggag
    25081 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accactt
//
LOCUS       DAZFFG010000071        24190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFFG010000071 DAZFFG010000000
VERSION     DAZFFG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.071"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            30..554
                     /locus_tag="ACHJ7J_003129"
     CDS             30..554
                     /locus_tag="ACHJ7J_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5001324.1"
                     /translation="MMSFEIKEWFNAKELEGMPGVPKLATNITRKAVAEDWVKRQRHG
                     GKGVAYEYHINSLPEETRRAIKGASLSDKPVHTSIAHTVDERLIYAMSFLTPDEQAAA
                     VEIIRVAGIKGLMPTIVSKDKALEALGITVEQQKTLQTLQALPPEKVREILSQYEGKE
                     HNFPARENDVKKAV"
     gene            complement(893..1276)
                     /gene="eutH"
                     /locus_tag="ACHJ7J_003130"
                     /pseudo
     CDS             complement(893..1276)
                     /gene="eutH"
                     /locus_tag="ACHJ7J_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
     gene            complement(1393..2580)
                     /gene="eutG"
                     /locus_tag="ACHJ7J_003131"
     CDS             complement(1393..2580)
                     /gene="eutG"
                     /locus_tag="ACHJ7J_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO5001325.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVVLLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDTVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(2570..3406)
                     /gene="eutJ"
                     /locus_tag="ACHJ7J_003132"
     CDS             complement(2570..3406)
                     /gene="eutJ"
                     /locus_tag="ACHJ7J_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO5001326.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDREGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLVIASSGREKAEGLY
                     AK"
     gene            complement(3417..4820)
                     /gene="eutE"
                     /locus_tag="ACHJ7J_003133"
     CDS             complement(3417..4820)
                     /gene="eutE"
                     /locus_tag="ACHJ7J_003133"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO5001327.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(4832..5119)
                     /gene="eutN"
                     /locus_tag="ACHJ7J_003134"
     CDS             complement(4832..5119)
                     /gene="eutN"
                     /locus_tag="ACHJ7J_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO5001328.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(5226..5519)
                     /gene="eutM"
                     /locus_tag="ACHJ7J_003135"
     CDS             complement(5226..5519)
                     /gene="eutM"
                     /locus_tag="ACHJ7J_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO5001329.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(5558..6574)
                     /gene="pta"
                     /locus_tag="ACHJ7J_003136"
     CDS             complement(5558..6574)
                     /gene="pta"
                     /locus_tag="ACHJ7J_003136"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5001330.1"
                     /translation="MIIERCRELALRAPARVIFPDALDQRVLKAAQYLHQQGLATPIM
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(6571..7374)
                     /gene="eutT"
                     /locus_tag="ACHJ7J_003137"
     CDS             complement(6571..7374)
                     /gene="eutT"
                     /locus_tag="ACHJ7J_003137"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO5001331.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGD
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMTILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(7371..8072)
                     /gene="eutQ"
                     /locus_tag="ACHJ7J_003138"
                     /pseudo
     CDS             complement(7371..8072)
                     /gene="eutQ"
                     /locus_tag="ACHJ7J_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
     gene            complement(8047..8526)
                     /gene="eutP"
                     /locus_tag="ACHJ7J_003139"
     CDS             complement(8047..8526)
                     /gene="eutP"
                     /locus_tag="ACHJ7J_003139"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO5001332.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVIKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(8539..8874)
                     /gene="eutS"
                     /locus_tag="ACHJ7J_003140"
     CDS             complement(8539..8874)
                     /gene="eutS"
                     /locus_tag="ACHJ7J_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO5001333.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(9166..11445)
                     /gene="maeB"
                     /locus_tag="ACHJ7J_003141"
     CDS             complement(9166..11445)
                     /gene="maeB"
                     /locus_tag="ACHJ7J_003141"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO5001334.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            11734..12684
                     /gene="tal"
                     /locus_tag="ACHJ7J_003142"
     CDS             11734..12684
                     /gene="tal"
                     /locus_tag="ACHJ7J_003142"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5001335.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            12704..14707
                     /gene="tkt"
                     /locus_tag="ACHJ7J_003143"
     CDS             12704..14707
                     /gene="tkt"
                     /locus_tag="ACHJ7J_003143"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5001336.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGKKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     ISLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(14802..15845)
                     /gene="ypfG"
                     /locus_tag="ACHJ7J_003144"
     CDS             complement(14802..15845)
                     /gene="ypfG"
                     /locus_tag="ACHJ7J_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO5001337.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRVFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(15971..16546)
                     /gene="nudK"
                     /locus_tag="ACHJ7J_003145"
     CDS             complement(15971..16546)
                     /gene="nudK"
                     /locus_tag="ACHJ7J_003145"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO5001338.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(16614..18593)
                     /gene="aegA"
                     /locus_tag="ACHJ7J_003146"
     CDS             complement(16614..18593)
                     /gene="aegA"
                     /locus_tag="ACHJ7J_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO5001339.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGDIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            18799..20499
                     /gene="narQ"
                     /locus_tag="ACHJ7J_003147"
     CDS             18799..20499
                     /gene="narQ"
                     /locus_tag="ACHJ7J_003147"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO5001340.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            20663..23776
                     /gene="acrD"
                     /locus_tag="ACHJ7J_003148"
     CDS             20663..23776
                     /gene="acrD"
                     /locus_tag="ACHJ7J_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO5001341.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(23875..23934)
                     /gene="ypfM"
                     /locus_tag="ACHJ7J_003149"
     CDS             complement(23875..23934)
                     /gene="ypfM"
                     /locus_tag="ACHJ7J_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO5001342.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         5590 a         6643 c         6258 g         5699 t
ORIGIN      
        1 tcactctatt gaaatcacta ggattttaaa tgatgtcttt tgaaatcaaa gaatggttta
       61 atgcgaaaga gctggaaggg atgcctggtg taccaaagtt ggcgactaac attaccagga
      121 aagctgtggc ggaagattgg gtaaaacgtc agcgtcacgg tgggaaaggt gtcgcttatg
      181 agtatcacat caacagtctg ccagaagaaa cacgtagggc cattaaagga gcctcacttt
      241 ctgataaacc agtgcataca tcaatcgccc atacagttga tgagaggcta atttatgcaa
      301 tgagcttctt aacaccagat gagcaggccg cagctgtgga gatcattcgt gtagcaggga
      361 ttaaaggact tatgcctaca attgtcagta aagataaggc attagaggca ttagggatta
      421 ctgtggagca acagaaaacc ctgcaaactc ttcaggcatt accaccggaa aaagtaagag
      481 agattttgtc tcagtatgaa ggcaaagaac ataatttccc tgcaagagaa aacgatgtaa
      541 agaaagccgt ataacaacca ggtaatagct aagacataaa cttcactcca ggtaattaca
      601 tatgtaagat gcccaaacag tgatgattgt ttttgacaca catcacttgc tgaaaacctg
      661 gttgttaccg tttttgaagc atttttgaag ctgtacttga aatgatgcgt gaagtaattg
      721 cttttcgtgt ctttaatgaa tcaaaagctg tttccaataa tctttcgcgt ttcaagatgt
      781 aacggctaaa atctgtccta caggcttgca agccccacta aatctaatcc cgccaaatcc
      841 cgaataatcc aatgtcctcc cggttttttt cgtacttcaa gtgaatcaat acaccgagaa
      901 ttaacccaga gtatagccac gctgctacgt cgccgcccgc cagctctttg gcgaggaaga
      961 agccgcccat atcgcacgcc agcagcgttc cggcgaacat tgacgggttt gcgccgagca
     1021 tttcgtaaac cgggataatt accggcccga gtacgtgagc cagtaccggt gccagcgcgg
     1081 tcataccgac catcgccaga cccagagcgc ccattgccat aaagccttct tcgaactgac
     1141 cgcctgatcc ttcgatactt ttaccgaact taccgaggaa acgagcagaa ccgccgaact
     1201 gcgacaggat cctgtctacg gcagctatca gcataaagaa catcatgatg tacatgatga
     1261 tttcgttaat tcccatcgcc tttactccct gttagttgtt atttattagc ggatgcggcg
     1321 tgaacgcttt atccgctcta catgtgcaat cccgtaggcc ggataagacg cgacatgcgt
     1381 cgcatccggc atttattgcg ccgctgcgta caggccgaca atctgttcca ggctggcggt
     1441 acgcgggttg ctgcgcagac aaatatcttc ctgcgcggcc tgcgcccatg caccgtaatg
     1501 cgcagatgtc gcgccaacat cgcccagtcg tttaccaatc ccaacttccg caatcagctc
     1561 acttaccgcg ttaatagcgt cacgatcgtc ggatttttta gttcgcagtg cccgaccaat
     1621 ctgactaaag cgttcacgac aaaccatccg gttaaactcc atcaccgttg gcagcaacat
     1681 agcgttcgcg agaccgtgtg gaatatgcag cgccgcgccc ggctgatgcg ccatcgcgtg
     1741 gcacaaccca agacccgcac tggaaaacgc cattcccgcc atacatgaag ccagcaacat
     1801 gctctcgcgc gcggcaaggt cgtgaccgta gcccaccgct ttcggcagcg atttgccaat
     1861 catcgcaatg gcaccaatcg ccagactgtc ggtaaacggt gtagcgttca gggcgctgta
     1921 tgcttcaatg gcatgggtta acgcatcaat gccggtcatc gccgtgacat gcgacggcac
     1981 accttcggtc aatgcggcgt cgaggatcgc cacatccggc atcagcgagg catgggctaa
     2041 cacctgcttg cgcccgctca ccgtgtcgat aatcaccgtt acattggtgg tttcagagcc
     2101 ggttccggcg gtagttggaa tggcaatcag cggcaagcgc ggttgcagaa cgctggtttc
     2161 tgacatctct gccagcgtgc tatccgggtt cgtcaccagc aacaccacgg ctttcgccgc
     2221 atccagcacc gagccgccgc caaatgcgat caccccgtca caacctgact cacgcaactg
     2281 cgccacggct gcacacacgt cggtaatgca cggttcgccc accggacatg gccagagcgt
     2341 catggcgata cctttaacgg ccaggctacg cgtcagcccg gcggtcatcc ctgcctgatg
     2401 caaaaagctg tctgccatca cgaacagatg tttcagccca cgcgtttgcg cttgctgtcc
     2461 gcaactgctc accgagcccg gaccgcaaag cgtcaccggt ggaacgctaa atgtttttac
     2521 ccgttgcaga ttcagggtat cgaacgcctg aaagagcgcg gtctgcaatt cattttgcat
     2581 atagcccctc cgctttctct cgcccactac tggcgatcac cagcggggtc ataaacaaac
     2641 tgtgctgcgg taaatgcacc tgtaacgccg ggaattgttt gcgaaacagt tccgccacgc
     2701 ccggctgcat acaggagccg cccgccagcc ataaatcagt aatgccctgt ccttcaatat
     2761 gccgggcgac aatgtccgcc attttttcgt acaccggttt caccgcgggc caaatctcgt
     2821 caccgtgacc gcgcttgtac tgctctgcct cttccagcga aatacggcga tttccggcga
     2881 gagtcagaga gatgtgatgt ccgccggttg cttcatccgc cgagtacgtc accttgccct
     2941 ttttcacgat ggcaatgccg gtagtgccgc cgccgatatc cactacaccg gcgttgtcca
     3001 gttgcagcag atccgcgact gcggttggct catccagcac atggctcact tccagtccag
     3061 cagactccag cacgttgatg gaaatacgcg ggtccgtacc tggaggaaat gaagtcgccg
     3121 catggctaaa acggcgaccg aattgctgct caagagtgtc gagatggcga cgaacaatgg
     3181 tgacagcgcc gaagaaatcc cagacgatgc cgtcgcgaac cacatcggcc cagtcgaggc
     3241 ataccgccac tggctgaccc tcgcggtcga caaccatcga caccacatcg catgtgccca
     3301 gatccacccc cagccacagc ggagattccg tcgcggcggg cgtctggtta cacagcgttg
     3361 ccgccgtttg cagacgtggg gtgagccatt gttcgtcgtg cgccatcggt tactccttaa
     3421 acaatgcgaa acgcatcgac taatacacag cgacgcagac ggacaaacgt acgcgcgctg
     3481 gttacccctt caccggttgg cgtggtgatg gtcatggtgg tccagccttc cccgcccagc
     3541 cccagcccgg caatgcacgg tccgttctta acgaaaatgc tggtatcaat agcattcgcc
     3601 atctggttca tgttttcgat gttgcgcgag tgcattgccg ccgtgtggtg gcaaccgccc
     3661 tccagtttca ccgctagcgc aatggcatcc gccacgttgg cgacgcgcac gacgggcaac
     3721 accggcatca tcagttcagt cacggcaaac ggatgttctg cggtggtttc tacaaacagc
     3781 aggcgcgttt cttgcggaac tttaaggccg attgccgccg cgattttgcc tgcgtcgcga
     3841 ccaacccagt cacggctgac ggtgcctttt ccgcgctcgt cgatattttt cagcaacacc
     3901 ggttgcagct gctgcgcctg ttctgcggtc agtttcaccg cgtgctggcc ttccatcaga
     3961 cgcatcagtt catcggctac gctatcaaca acaatcagta ccttttcgtc ggcacaaatg
     4021 atgttgttat cgaaagacgc gcctttgacg atggactgag cagcacgggc gaggtcagcg
     4081 gtttcatcca ccactaccgg cgggttgcca gcgcctgcgg caatcagacg tttattggtg
     4141 tgtttacgcg ccgcttctac taccgcttcg ccgccggtca ccaccagcag gccgataccc
     4201 ggaaacttga acaagcgttg cgcggtttcg atatccggat ttgccacagt aaccagtaag
     4261 ttttccggcc cgcctgcggc aacaatcgcc tggttgagca gcgtgatcgc ccgctgggag
     4321 acttttttcg ccgccggatg cggggcaaaa atgacgctgt tgcccgcggc aatcaggctg
     4381 atggcgttgt taattacggt tgccgccggg ttagtggaag gcgtcaccga agccaccacg
     4441 ccccagggtg cgttttcaat tagggtcagg ccgttgtcgc cagtcagcac ttgcggagag
     4501 aggcactcaa cgcctggtgt gccgcgcgcc tgagcgacgt tttttgcaaa tttatcttca
     4561 acgcgcccca tgccggtttc actgacggca agttccgcta aatctctggc gtgtttttcg
     4621 cctgcttcac gaatggcagc aatggctaac tggcgcattg ccacgctctt taacccttgc
     4681 tgggcaattt tggctgccgc aacagcgtca tccagggacg cgaaaacgcc catctcatga
     4741 acggcggcgg gcggcgtgtc actgctttgc attttcagca gtaccgcttt caccacctgt
     4801 tcaatatcct gttgattcat gatgttctgc cttatttgtg gaaaattacc tgaccgccag
     4861 acaccacctc atcgacgatg ccaatcacgc acagatcgac cggtgacgtt tcgcttttat
     4921 gcgcctggcg ggcggaactg ccactcacca gcaacaccca ctccccggtt cccgcgccaa
     4981 tattgtcgat ggcgacggca cattgcccgt caggattacc ttgtggatca atcatttcca
     5041 ccatcagcaa tttgtcatgc gccagtccgt gatggcgtac ggtacaaaca atttgtccag
     5101 tgacgactgc cagtttcata cccgcctccg tggcgtattt caggtaaaag ctccccctac
     5161 cctccgcgga aggtaaaatg aaaaaggaga gagcgtgacg cccgaatcga cgtcacacag
     5221 ggtgattaca ggttgctgct atcgcctttc aggccgatcg ggaacacttc ttccaggtcg
     5281 ccgtgcggac gcgggataac gtgtacagat accagctcgc cgatacgctg tgccgctgcg
     5341 gcaccggcat cggtcgccgc tttacaggct gcaacatcac cacgaaccat ggcagtacac
     5401 aggccgccgc caatctgctt aacaccaacc agcttgacgc gcgcagcttt caccatcgcg
     5461 tcagaagcct caatcagtgc aaccaggccc cgggtttcga tcattcctaa tgcttccatt
     5521 gtgctttcct ctttatcagg gtccagaacg ggaccgttca ttcaaccagt gtttgtaaac
     5581 tgctttcgcg gttcacttct gtctgacgcg gcacagctgc caccagcgcc agctcgataa
     5641 tttcctgcac actacaacca cgagagagat cgtgcatcgg cgcggcaagt ccttgtatca
     5701 atggcccgac ggcacgatat ccgccgagtc gttgtgcgat tttgtagcca atatttccgg
     5761 cttccagcga cggaaaaacc atcacattgg ccttgccctg tagcgggctg gcaggcgctt
     5821 tttgcgccgc cacttccggc acgaaggcgg cgtcaaactg caactcgcca tcgactacca
     5881 gctttggtgc gcgctcacgg acgatttctg tcgcctgctg gacattggca acgcaggggt
     5941 gacgggcgct accgttactg gaaaacgaca gcatcgccac gcgcggctct tctccggtga
     6001 tggcgcgcca ggtttcggcg ctggcaagcg cgatatccgc cagctgcgcc gccgtcggct
     6061 gtggtaccac gctgcaatcg gcaaagccca acgcggggcc actatactgt ggcagcatca
     6121 ggaaaatgga agagagcgtt ttacagccag gttgcaagcc aataatgcgt aatccggcac
     6181 gcagcacatt cgccgtggaa gagaggttgc ccgcgataca gacatccgct ttaccggcgc
     6241 tgaccattgc ggcggcgaac atcagcggat cggtaagttt ttccagcgca tccggcggcg
     6301 ttttttcgcc cgcgcgggcc agccagcgat gagcaaattc ttcccgcatt gcgaggttgc
     6361 catgcggatc aataacctgt agcccgtcca tcgctacgcc atgactgagc gcaaactgac
     6421 gaagttcaaa cggattggca accataatgg gcgttgccag accttgttga tgtaaatatt
     6481 gcgccgcttt cagcacacgt tgatctaacg catccggaaa aatcactctg gcgggcgctc
     6541 gcaacgccag ttcacgacaa cgttcaataa tcattgcgtt tcccccagtc gttgttggat
     6601 ctgcttcacc gttagcggcg gcttcgttac gctcaggatc gtcatcacgt agaccgtgct
     6661 cgaaaggcgg ttcagcgcct gcaagatgtc cgggcgtaat acttcaaagc tgcgggtgat
     6721 aaacacctgc gccgctacgg tttcggtttc ccgcactttg gtgcgtagca gattgagcaa
     6781 cgcagcatcg cgcccgtggc tggcttccgg caccagatga tcgtggtcga gatagcgcag
     6841 cggctgatga gaaagccgat gcagatcttc gtcagaaagc ccgacaatcg cttgatcgcc
     6901 cagcggttcc cccagcgcat cggcacgcat aatgttgcca agacgcgaac ggatatccgc
     6961 aagccacggc tgccacggct ccgccagttc aatttgcagc cacactgcca gcgcaatggt
     7021 gctgtcgaga accgcgcgaa aacccaggcg cggatcgctt ttggcgacca ttttttccgc
     7081 cgacagatga gtcagcgtat ccggcttttt tgccaccggc tggcggcaca gttcgcagca
     7141 cgcctgcgga tgttcatcgc tgctggtcag cccatgaacg ggctgcggct gctgctgttc
     7201 atcgtcaaca aacaggcggc cctgctcgtc aataaacttg atgcgcagat ggcggctttc
     7261 cagcaactcc cgggcagagg gcgtcaggcg actgtccgct gggagatgga tctctgcgcc
     7321 ttcgctgagc gtatggttcg ctcttagcca tgcttcggtg atgaaatctt tcatagggat
     7381 tgccagttag ccggccaggc gacatacagg aatttcacgc tggatgtcgt accaaattcg
     7441 atgctggagc ctttcgggat aaacattacg tcgcctgctt tggcgatcat ggtttcgcct
     7501 tcgtggcgaa catgcagctc cccttccagc accatgtcga tttcgtcgta gttcagggtc
     7561 cacgggaaga atgcgttctc ccactgcata aaaccggcgg ccatgctgct gccgtcgtct
     7621 ccggtcacaa ggtcagttaa gcccacgcaa tgcggctctg cgccgtcgaa acgaccaaac
     7681 ttgacgctgc tgccatcaat cactttgatg ccgcctttgc cggtcaccga tttgaagctc
     7741 ggctgcattg caccctgctc cagcgactgt ttttccttca tcactttttc catcagctgc
     7801 gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg caatgatggt ttcgcggatg
     7861 cgctagcttt cggttttatc ggctggcacg ctggcgggca cggacgccgt taccgggatc
     7921 gattcatcac attcggtgat ggtaaaaccc agtaaatccg ccacttctcg ggcttccggg
     7981 gtaataatgc tggcgcgcag aaccacggac attgcctgtt cgccgcgcgc gtgtgcttca
     8041 cgaatatcat tcgctgtgat gagttttttc acctgcttcc tcctgtttgg tgagagatgc
     8101 cagataatcc accagttgct gcacgctttg cgggtcgtgg ctgtttagct cgaaaatcgg
     8161 ctcttcgaag cccgtctcaa gcaataactt tcgcgttgcg gcgacgtcag catctggcat
     8221 gtccgtttta ctgatgacgg cgatttgccg tttgatgacg ccaatatcca gcaacccggc
     8281 aggcaaccga ctttccgggt cattcgcgcc gtggacatag atcagcatgt ccacatcctg
     8341 tagcgtggta attaaggcgt gataccagcg gggatggcta aaatattcac ccggcgtatc
     8401 aatatcgccc ttatcattaa attccacggc ctgtgttttt ctggcgaggg tataattccc
     8461 ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg gtacccacaa acgcaattcg
     8521 tttcatgaat gcctcgtatt aacttttggt catttcacaa agcgtataat ttaataagcg
     8581 acctaaaccg ctgacggttt gtgataacgc ttcttctact gcgcccaccg agccataaat
     8641 caccaatgca ccactgaagc ggtcgagaaa accgatatgc acatcagcag ctttcaatgc
     8701 taaatcaccg gcgatcatcg ccgtttcgcc aggagttagc gtcataatgc caattgcgcc
     8761 cgcatccgga acgccgattt tcttcgccag ttcctcgccg ggatgcgcga tgagatgcgc
     8821 cagcgtgacc tgtttgccgg gcacaaattc ctgaatgatg cgttctttat ccatgagtcg
     8881 ccgcctgtga aaaacttgcg tgtatcgtga caatttttcg tcagagtaaa taacaaaatt
     8941 cggcaagcgc gtttaaaagg tgaggtagat cactaagaat ggagagatat ttactgttat
     9001 gaataaactt tcatatgcga cgcatgaata taaataaaat aatatcgaga tattttatat
     9061 tgcttttttt attattcgta aactcagtaa taagctttac taatatattg cagctaaacc
     9121 gcttaccctg aatattcagg gtaagcgtga gagttaaaaa aagaattaca gcggttgggt
     9181 ttgcgcttca accacggcca gcgccaccat gttgacgata cgacgtaccg atgcgatcgg
     9241 cgttaacacg tgaaccggtt tcgccacgcc catcagcacc ggaccgacag tcacaccttc
     9301 cgagctggaa acgcgcagta agttgtaact aatgcgggca gcttccatgt tcggcatcac
     9361 cagaatattg gcggaacctt tcaaagggct gtccggcata cggtcgttgc gaatcgcttc
     9421 caccagcgct gcatcgccgt gcatttcacc atcaatcatc agttctggtg cacgttccct
     9481 gaccagttcc agcgcctgac gcattttgct cgacgacggg cagtcagaag aaccaaagtt
     9541 ggagtgcgac aacaaagcaa cgcgcggctc aataccaaaa cgacggacag tttctgccgc
     9601 catcaaggtg atctccgcca gctcttctgc gtctggttcg tcattgacgt aggtatcggc
     9661 aataaaggtg ttaccactcg gcagcagcag cgcgttcatt gcacctgcgg tgtgaacgcc
     9721 atcgcgataa ccaaagacat ttttcaccac gctaaaatgt tcatgataat cgcccaccgt
     9781 accgcagatc attgcatcgg cttccccacg ctgaaccatg atcgcgccga tcactgtcgg
     9841 gttactgatc agcgcacgct gcgcctgttc ctgagtgaca ccgcgacgct tcatgatctg
     9901 gaagtattcg gtccagtact ctttaaagcg cggatcggat tcgttattga cgatctcaaa
     9961 atcaacgccc gctttgatct gcaagcccag tttctgaatg cgcatttcga tcacgttcgg
    10021 acgaccgata aggatcggtt tcgccagtcc cagcgttacc agttcctgag tggcatgcag
    10081 aacgcgcgcc tcttcccctt ccggcagaac tacgcgcttc ggcgctttgc gagcctggga
    10141 gaaaatcggc ttcataaaca ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta
    10201 gacgtcgaaa tcagcaatcg gacgcgtcgc cacgcccgac tccatcgccg ctttcgcgac
    10261 cgcaggagca atcttgacta tcaagcgcgg atcaaacggt tttggaatga tgtattccgg
    10321 accaaagcta agatcctgat cgccatacgc agaagccacc acttcgctct gttcggcgtg
    10381 ggccagttcc gcaatcgcgc gtaccgccgc cagtttcatc tcttcattga tagccgttgc
    10441 gccaacgtcc agcgcgccac ggaagataaa cgggaagcaa aggacgttgt tgacctggtt
    10501 tggatagtca gaacgaccgg tgcagatgat ggcatccgga cgcacttctt tcgccagcgg
    10561 cggcagaatt tccggttccg ggttcgccag cgccaggatc attggcgctc gagccatttt
    10621 cttcaccatt tcctgggtca gcactttcgg gccggaacag ccgaggaaaa tatccgcgcc
    10681 ttcaatcaca tcatcgaggg tacgtttgcc gtcatccact accgcatatg cggctttggt
    10741 ttccgccatg tttggctcac ggccctgata gataacgcct tttgaatcgc aaaccacgat
    10801 gttatgtttt tgcagaccca gcgcgaccag caggttcata caggcgattg ctgcagcacc
    10861 cgcgccggaa accaccatac gcacgtcaga gatgtttttc tccaccacgc gcaagccgtt
    10921 gaggatggcg gcagtgctga tgattgccgt gccgtgctga tcgtcgtgga ataccggaat
    10981 attcatccgc tcgcgcagtt tctgttcaat atagaaacat tctggcgctt taatgtcttc
    11041 gaggttgatt ccgccgaagg tcggttcgag cgcagcgacg acttcaataa atttgtccgg
    11101 gtcgagttca tcaacttcaa tgtcaaatac atcaatcccg gcgaatttct taaacagaac
    11161 gcccttgcct tccatcaccg gtttgcctgc cagcgcgcca atgttgccta accccagcac
    11221 cgccgtaccg ttagagatca ccgccaccag gttaccgcgc gcggtatatt tgtaggcttt
    11281 taacgggtct ttttcgattt caagacaagg tgcggcaacg cctggtgagt aggccagcgc
    11341 cagatcgcgc tgcgttgcca gaggcttggt tggagaaacc tggattttcc ctggaactgg
    11401 aaattcatgg aaatcaagtg cactttgttt taactggtca tccatttggt tgttcctttc
    11461 acgtaacgtt cacaaataaa gtgtgtgggc aacagcccct gcccacaacg tggcgcacat
    11521 tattaccttg ccggagtcta cagactttga gcaagtccaa actctcacca ttaatataat
    11581 gttttggtaa taatcctata acactgatgt tacctgctta atccagcaat accatgcctg
    11641 tctgctatgc ttttttgatg cgtttagcga aatttctcag aagtgtgaat taacgcactc
    11701 atctaacact ttacttttca aggagtattt cctatgaacg agttagacgg catcaaacag
    11761 ttcaccactg tcgtggcaga cagcggcgat attgagtcca ttcgccatta tcatccccag
    11821 gatgccacca ccaatccttc gctgttactc aaggctgccg gattatcaca atatgagcat
    11881 ttaatagacg atgctatcgc ctggggtaaa aaaaatggca agacccagga acaacaggtg
    11941 gtcgcagcgt gtgacaaact ggcggtcaat ttcggtgctg aaatcctcaa aatcgtaccc
    12001 ggtcgcgtgt caacagaagt tgatgcacgc ctctcttttg ataaagaaaa gagtattgag
    12061 aaggcgcgcc atctggtgga cttgtatcag caacaaggcg ttgagaaatc acgcattctg
    12121 atcaagctgg cttcgacctg ggaaggaatt cgcgcggcag aagagctgga aaaagaaggt
    12181 attaactgca acctgacgct gctgttttct tttgcccagg cacgggcctg tgcagaagca
    12241 ggcgtttttc tgatttcgcc gtttgtcggg cgtatttatg actggtatca ggcacgcaag
    12301 ccgatggacc cgtatgtggt ggaagaagat ccgggcgtta aatcggtgcg caatatctac
    12361 gactactata agcaacacca ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc
    12421 gaacaaatcc tcgccttaac cggctgcgat cgactgacta tcgcaccgaa tttgctgaag
    12481 gagctgcagg aaaaagtttc gccagtggta cgtaaattaa tcccaccttc tcagacgttc
    12541 ccacgcccag ctcccatgag cgaagcggag ttccgttggg agcacaatca ggatgcgatg
    12601 gcggtagaaa aactgtctga aggcattcgt ctgttcgccg ttgatcaacg caaactggaa
    12661 gatcttcttg ccgccaaact ataaaccagc cacggagtgt tatatgtccc gaaaagacct
    12721 tgccaatgcg attcgcgcac tcagtatgga tgcggtacaa aaagccaatt ctggtcatcc
    12781 cggcgcgccg atgggcatgg ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca
    12841 taaccctacc gacccaacct ggtatgatcg cgaccgcttt attctttcca acggtcacgc
    12901 gtcgatgctg ctctacagtt tgctgcatct gaccggttac gacctgccgc tggaagagct
    12961 gaagaacttt cgtcaactgc attcgaaaac cccaggccac ccggaaatcg gctatacgcc
    13021 cggagttgaa accaccaccg gcccgcttgg acaaggtttg gcgaacgccg tcgggctggc
    13081 gatagcagag cgtacactgg cggcgcagtt taaccagcca gaccatgaga tcgtcgatca
    13141 cttcacctat gtgtttatgg gcgacggctg cctgatggaa ggtatttccc acgaagtctg
    13201 ttcgctagcg ggcacgctgg gactgggcaa gctgattggt ttttacgatc acaacggtat
    13261 ttcgattgat ggagaaaccg aaggctggtt taccgacgat acggcaaaac gttttgaagc
    13321 ctatcactgg catgtgatcc atgaaatcga cggtcacgat ccgcaagcgg tgaaggaagc
    13381 gatccttgaa gcgcaaagcg tgaaagataa gccgtcgctg attatctgcc gtacggtaat
    13441 aggctttggt tcgccgaata aagcaggtaa ggaagaggcg cacggcgcac cactggggga
    13501 agaagaagtg gcgctggcac ggcaaaaact gggctggcac catccgccat ttgaaatccc
    13561 taaagagatt tatcacgcct gggatgcccg cgaaaaaggc aaaaaagcgc agcagagctg
    13621 gaatgagaag tttgccgcct ataaaaaggc tcatccgcaa ctggcagaag agtttacccg
    13681 tcggatgagc ggtggtttac cgaaggactg ggagaaaacg actcagaaat atatcaatga
    13741 gttgcaggcg aatccggcga aaatcgctac ccgtaaggct tcgcaaaata cgcttaacgc
    13801 ttacgggccg atgctaccgg agctgctcgg cggttcagcg gatctggctc ccagtaacct
    13861 gaccatctgg aaaggttcta tttcgctgaa ggaagatccg gcgggcaact acattcacta
    13921 cggggtgcgt gaatttggca tgaccgctat cgccaacggc attgcgcacc acggcggctt
    13981 tgtgccgtat accgcaacgt tcctgatgtt tgttgaatac gcccgtaacg ccgcgcggat
    14041 ggcggcactg atgaaagcgc ggcagattat ggtttatacc cacgactcaa ttggtctggg
    14101 cgaagatggt ccgacgcacc aggctgttga gcaactggcc agcctgcgct taacgccaaa
    14161 tttcagcacc tggcgaccgt gcgatcaggt ggaagcggcg gtgggctgga agctggcggt
    14221 tgagcgccac aacggaccga cggcactgat tctctccagg cagaatctgg cccaggtgga
    14281 acgtacgccg gatcaggtta aagagattgc tcgtggcggt tatgtgctga aagacagcgg
    14341 cggtaagcca gatattattt tgattgccac cggttcagag atggaaatca ccctgcaagc
    14401 ggcagagaaa ttagcgggag aaggtcgcaa tgttcgcgta gtttccctgc cctcgaccga
    14461 tattttcgac gcccaggatg aggaatatcg ggagtcggtg ttgccttcta acgttgcggc
    14521 tcgcgtggcg gtggaagcag gtattgccga ttactggtac aagtatgttg gtctgaaagg
    14581 ggcaattgtc gggatgacgg gttacgggga atctgctccg gcggataagt tgttcccgtt
    14641 ctttggcttt accgccgaga atattgtggc aaaagcgcat aaggtgctgg gagtaaaagg
    14701 tgcctgatgg tgatcgccgg atgctgattg ccggatgcga cgctgacgcg tcttatccgg
    14761 cctacagcgt cttatccggc ctacatgtcc cgccattttg tttaacgggt gatccacaac
    14821 gtgggccagg cgtctggccc atgccagtta tcgcaggtgg gttctgcggc gtaacgcacc
    14881 aggcgaaaac gctgaccgtc aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg
    14941 cgccctttcg ctaaggtcac cagttcacgc gatttctcat caaatgttgc gttcatcagt
    15001 tccagttcat ttgtctcctg cccgttgttg aacggcaaac gcaaccgaac cggacgcgag
    15061 gctagtggct ttttacgcga cacaatccat gccaaatcaa tggtgttata ggcccctgcc
    15121 tcacagctaa tcatcatcag cgctttatca tcagtcagcg cagtgacatt cacctcacga
    15181 cgcaatggat caagcgagca gcgcagacca ttcatccgcc agtttccata atccagcaaa
    15241 tcgttgcgtt cttcgagtga gagtggcgtc ggcgttgggt taaccaccgc gacctctttc
    15301 agcgcaggcg cgggcggtac gctgagcggc ggttcgtccc ctttcttgat ccacgcggtt
    15361 tcactgccaa cgcgcttttg ctgagcatca ataaacaaca acgccgcttt taagccactc
    15421 agagaaatgg tctgattgcc gtcgcgtaag gtgatcgcct tcccttcctg aatcatctgc
    15481 aaaaacgcgg tgatggttgc cgtatcgtcg gttactaata accatggtga aatccgccac
    15541 ttgtcaccac ttaacgccaa cggctcgcca tctaacagca gccgtggcgc aatttcccct
    15601 tcagacgcat ccggcgactt caacccgccg cgctcaatac gtaaaacggc atcggtatgc
    15661 gccccggcgc tgcggctcag ggtcatcacc agtccattat gatcgcccgt attacgcgcc
    15721 acgcagaagt tttggttatt acaggtgacc tgccagtcgg aaaacacccg ttgcgctggt
    15781 gccgcccaca ccaaagacgt cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag
    15841 cgcatggacg gtacgacccc agaagttcaa acaaacagca caagccgtat cttcgttgcg
    15901 caggtagcgc ggctcaatcg gatttgtcgg attgagccaa caataaacaa cgaaatatta
    15961 ttgttatttt tcagtccatt aaatgtgacg tttgcaaata gttaagcaat aacaccgtct
    16021 taccgtcacg tatctcgccg gttttgatca tctccagcgc ctggctgaac ggcagctcga
    16081 gcacttcaat atcttcatct tcgacaccgc caccggcgtt agcgcgctga ttgtcactgt
    16141 attcggcgat aaaaaagtgg attagctcag tcacaccgcc tggcgacata tacagttcaa
    16201 ataatttgcg cacttcgccc acttcatagc ccgtctcttc aatcgcttct ttgcgaatgc
    16261 acacttccgg ttcgtcgtta tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt
    16321 cattgccatt aacccaggta gcgacacgga actgacgaat cagaaccacg gtctttttct
    16381 tcgcgttgta caggaggatc gtcgcaccat taccgcgatc gtaaacttca cgtttatggc
    16441 ggataacttc gccatctttg cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt
    16501 tatcggagag aattttgtct ttaatgaggg tgatttgttg cgtcataccg actccacagc
    16561 gcgaaatgaa caattatctt acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga
    16621 gatttgactg attttacccc cagccagtca ataatcccct gtgccgcatg acgtccttct
    16681 gccattgcgg taaccaccag atccgcacca cgcacggcgt cgccaccggc gaagattttc
    16741 ggattggtgg tctggtaacg gtactggctt tccacatccg cgatgatgcg gccccatttg
    16801 tctaccgtta caccgtgcga ctccagccac ggcatcccgt gcggattgaa gccaaacgcc
    16861 ataatcaccg cgtcggctgg catgacaaat tcactgcctt ccaccggcac tggacgccta
    16921 cgcccctggg catccggctc gccaagacgc gtgcgcagga aacgaatccc gcagacgtga
    16981 ccttgttcat tcagctcaag cgccaccggc tggacgttaa attcgaagtt ggccccctct
    17041 tcgcgggcgt tcttcacctc tttcttcgag cctggcatgt tagcttcatc acgacgataa
    17101 gcgcaggtga cgttactcgc gccgtggcgc agcgcagtac gcacacagtc catcgcggtg
    17161 tcgccgccgc ccagtaccac gacgttaagt ccggcggtat tgataaacgg ctcttccggt
    17221 agctcttcga gccccatcac ctgtttagtg ttggcgatga ggaacggcag cgcgtcataa
    17281 acgcccggcg catcttcatt gggtaaaccc gctttcatgg aacggtaagt gcctacgcca
    17341 acgaagaccg cgtcgtattg ttccaaaagc gaatccaaag agacatcttt acccacttca
    17401 caattgagtt cgaagtgaat ccccatcgcg ctgaagattt cccgacggcg tgccagcagg
    17461 gatttatcca gtttgaaaga aggaatgccg aaagtgagca agccaccgat ttctggatgg
    17521 cgatcgtaca ccgtcaccgc cacgccattg cgggtcagaa catccgcaca ggccagccct
    17581 gccggacctg caccgataat cgccacccgc ttgtccactt tggtgacatg gcttaagtca
    17641 gggcgccaac ctttcgccag cgcctgatcc gaaatgtagc gttcaatgtt gccgatagtt
    17701 accgcgccgt gctcatcgcg aatggtgcag gcaccttcgc acagccggtc ttgcgggcaa
    17761 acgcgcccgg taatttcagg cagggtgttg gtctggtgag aaagctcgac ggcggcgtcg
    17821 atatctccgg ctttcaccag ttcaatccac tgcggaatat ggttatgcag cgggcaggtc
    17881 cattcacaaa cgctatgctc gccgcactta aggcagcgcg aggcttcccg ttgtgcctga
    17941 tcggcacgga atggcagata aatttcatca aaaccggttt ttcgcgcttc aatcgccagt
    18001 ttatccggct cgccacgcgc gggcgttgcc tgcatttgtt cgactttact cattaccggc
    18061 atttcttgtg cagcggtact ggcatgccac ggttgatgtt cctgacgtgc ggtgcgcaag
    18121 cggcgggatt tcgccatgcc ggagagtgcg gcatcagtga ccagttgcag cgcgtccgcc
    18181 gggcaattct cgacacaggc aggaccattt tcgcgccctg cacaaaggtc gcatttatgc
    18241 gccgtggctt taactttgcc cgctgcgacg ggtgtcagga cgatttgcat cgtaccaaac
    18301 ggacaggcca ccacgcagga tttacagcca atacactttt gctgattgac ctgaatgctg
    18361 tcatcaacgt ggctgattgc gccattaggg cagctacggg cgcagggcgc atcttcacaa
    18421 tggtgacagg tcactgcact acgttgctgc tgatgtttga taaccgtaat tcggggatga
    18481 aaatgatgtt ggctcaggac atgttgctca tcattgtgag ccatgacaca agcgatttca
    18541 caagcatgac aacccagaca ttgctgactg ttggccataa taaaacgatt cataacgacc
    18601 ttcttttttg gttgtaaaaa ccttattctt tatatgagtg ttgttattac ccgacttaca
    18661 ggggaatcgg caatgttcat gtgcccagaa taagtaacta tttcgctata aactgtggca
    18721 gatcaaataa tcccatcact gactaaattg cgtttcagcg aactggaaca ttaatgattt
    18781 tttgtggaga agacgcgtgt gattgttaaa cgacccgtct cggccagtct ggcccgggcc
    18841 tttttttaca ttgtgctgct gtcgattctt tccacgggta tcgctctgct aactctggcg
    18901 agcagtttgc gcgacgctga ggctatcaat attgccggat cgctgcgtat gcagagttac
    18961 cgcctgggct acgacttgca aagtggcagt ccacaactca atgcacatcg ccagttattt
    19021 cagcaggcac tgcattcacc ggtattaacc aatctcaacg tctggtatgt gccagaagca
    19081 gtaaaaactc gctatgcgca tctgaatgcc aactggctgg agatgaataa tcggctcagc
    19141 aagggcgatt tgccgtggta tcaggccaat attaataatt atgttaatca gatagacctg
    19201 ttcgtgctgg ctttacagca ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc
    19261 ctggctggcg gcatcggtat tttcacgctg gtctttttta ctttgcgccg catacgccat
    19321 caggtggttg ccccgctgaa tcagctggtt accgccagtc agcgtattga acacggacag
    19381 ttcgactcgc caccgctgga taccagcctg ccgaatgagc ttggcctgct tgcaaaaacc
    19441 tttaaccaga tgtcgagcga gctacataaa ttgtaccgtt cgctggaggc gtcagtagaa
    19501 gaaaagaccc gcgatctcca cgaggccaag cgtcgtctgg aggtgttgta tcaatgttcg
    19561 caggcgctga acaccagcca gattgatgtg cattgtttcc gccatatttt gcagattgtt
    19621 cgcgacaatg aagcggctga atatctggag ttaaatgtcg gtgaaaactg gcggattagc
    19681 gaagggcaac caaacccgga attgccgatg cagattttac cggtgacaat gcaagagacg
    19741 gtttacggcg aactgcactg gcaaaatagt cacgtttcat catcagaacc gctgcttaac
    19801 agcgtttcgt cgatgctggg acgcggtttg tactttaatc aggcgcagaa gcattttcag
    19861 cagttattgt tgatggaaga acgtgcaacc atcgcccgcg aattgcacga ctcgctggct
    19921 caggtacttt cttacttacg tatccagttg acgttactga agcgttcgat accggaagat
    19981 aacgccaccg cacaaagtat catggccgat ttttcccagg cgttgaatga tgcttatcgg
    20041 cagttacgcg agctgttgac tactttccgc ctgacgctgc agcaggcgga tctcccctcc
    20101 gcgttgaggg aaatgctgga tacgttacaa aatcaaacca gcgccaaact gaccctcgac
    20161 tgccgtctgc caaccctggc actggatgcg caaatgcagg tgcatttgtt gcaaattatt
    20221 cgcgaagcgg tgctgaatgc gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc
    20281 gtcaccgcgc cggacggcaa tcacacggtt tatatccgtg ataacgggat tggtatcggt
    20341 gaaccgaaag aacccgaagg tcattatggt ctgaatatca tgcgcgaacg cgcggaacgg
    20401 ctaggtggga cgctgacttt ttcacaacct tccggcggcg gcacgttagt gagtattagc
    20461 tttcgctctg cggagggtga ggaaagtcag ttgatgtaat gcctcctact gaccaaagaa
    20521 tacttgcact taagattcag tataaaaggg catgataatt tacattaact cctttttttc
    20581 tccacgattg gctcgtacct tgccgctaca gtgaagcaag tcaagcctac aacgatacgc
    20641 agaaacacga ggtcctcttt taatggcgaa tttctttatt gatcgcccca tttttgcctg
    20701 ggtgctggca atcctgttgt gtctgacagg taccctggcg attttttcat tgcccgttga
    20761 acaatacccc gatctcgcgc caccgaatgt gcgagtgacc gctaactatc ccggcgcatc
    20821 ggcccagacg ctggaaaaca ccgtgaccca ggttatcgag caaaatatga ccggcctcga
    20881 taatctcatg tatatgtcat ctcagagcag tggcaccggt caggcatctg tcactttaag
    20941 ttttaaagca ggcaccgatc cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc
    21001 agccatgcga aagttaccgc aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg
    21061 cgataccaac attctgacca ttgccttcgt ctctaccgat ggttcgatgg ataaacagga
    21121 tattgctgat tatgttgcca gtaatattca ggacccgtta agccgcgtga atggcgtcgg
    21181 ggatatcgat gcctatggtt cgcaatattc catgcgtatc tggctggacc cggcgaaact
    21241 caacagtttc cagatgacgg ctaaagatgt cactgatgcc attgagtcac agaacgcgca
    21301 gattgcggtt gggcaacttg ggggtacacc ttccgtcgat aagcaggcgc tcaacgccac
    21361 cattaacgcc cagtcactgc tgcaaacacc agaacagttc cgcgatatca ccttgcgggt
    21421 gaatcaggac ggctcagagg taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga
    21481 gaaatacgat tatcttagcc gcttcaatgg taagccagcc tccgggctgg gggtaaaact
    21541 ggcctccggc gcaaacgaaa tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct
    21601 ggcgcagtac ttcccgcacg gactggaata caaggtggcg tatgaaacca cctcgtttgt
    21661 taaagcctcc attgaagacg tggtgaaaac gctgctggaa gctatcgccc tggttttcct
    21721 cgttatgtat ctgttcctgc aaaacttccg cgccacgctg atacccacta tcgccgtgcc
    21781 ggtggtgttg atgggaacct tctccgtact ttacgccttc ggttacagcg tcaacacctt
    21841 aaccatgttc gcgatggtgc tggcgatcgg tctgctggtg gatgacgcca tcgtggtggt
    21901 ggaaaacgtc gaacgtatta tgagtgagga aggactcact cctcgcgaag ccacacgtaa
    21961 atcgatgggg cagatccagg gggcactggt cgggattgcg atggtgcttt cggcggtatt
    22021 tgtaccaatg gccttcttcg gcggcaccac cggtgccatc tatcgccagt tctctattac
    22081 cattgttgcg gcgatggtgc tgtcagtact ggtagcgatg atcctcactc cggctctgtg
    22141 tgccacacta cttaagccac tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc
    22201 ctggtttaac cagatgttta accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat
    22261 tctccaccgt agcctgcgct ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt
    22321 cctgttcctg cgtttgccga cgtcgttctt accgctggaa gaccgtggca tgtttactac
    22381 ctcggtacaa ttgcccagcg gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat
    22441 cgagaaatat tacttcaccc atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg
    22501 ctctggccct gggggcaacg ggcaaaacgt ggcgcgaatg tttatccgcc tgaaagactg
    22561 gagcgaacgc gacagtaaga ccggcacctc atttgccatt atcgagcgtg caacgaaggc
    22621 gtttaacaag attaaagaag ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct
    22681 tggtagttct gcgggttttg atatggagtt gcaggaccac gctggagcag gtcacgatgc
    22741 gctgatggca gcacgtaacc agttgctggc gctggcggcg gaaaacccgg agctaacccg
    22801 tgtgcgccat aacggcctcg acgacagtcc gcagttgcag attgatatcg accagcgtaa
    22861 agctcaggcg ctgggcgttg ctatcgacga tattaacgac acactgcaaa ccgcctgggg
    22921 ttcgagctat gtgaatgact ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc
    22981 agctgcgccg tatcgcatgc tgccagatga cattaatctc tggtatgtcc gaaataaaga
    23041 tggcggcatg gtgcccttct ctgctttcgc gacctcacgc tgggagacag gctcgccgcg
    23101 tctggaacgc tataacggtt attctgcggt tgagattgtt ggggaagccg caccgggggt
    23161 cagtaccggt acggcgatgg atattatgga atcgttagtg aagcagttgc caaacggctt
    23221 tggtctggag tggacggcga tgtcgtatca ggagcggctt tccggcgcgc aggctccggc
    23281 gctgtacgct atttccttgc tggtggtatt cctgtgtctg gcggcgttgt atgaaagctg
    23341 gtcggtaccg ttctcggtaa tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc
    23401 aacctggatg cgcgggctgg aaaacgacgt ttacttccag gtgggcctgt taacggtcat
    23461 tggtttatcg gcgaaaaacg ccatcctaat cgtcgagttt gctaacgaga tgaaccaaaa
    23521 aggccacgac ctgtttgaag cgacgctcca cgcctgccgt cagcgtttac gcccgattct
    23581 aatgacctcg ctggcattta tcttcggcgt attgccaatg gcaaccagca cgggtgccgg
    23641 ttccggtggt cagcatgcgg tgggtactgg cgtaatgggc gggatgattt cagccactat
    23701 tctggctatt tacttcgtgc cgctgttctt tgtgctggtg cgccgccgct tcccgctgaa
    23761 gccgcgcccg gaataagcaa taaaaaaggc gacatgctaa tgtgtcgcct ttttcaactt
    23821 tccgattaag aacctgctca gcgggttctt gctgtttgta ctttgtctca ggaattactt
    23881 acgaagcata acttcgataa agtctttcca gttccccagt tcacgttcaa tcataacaac
    23941 ctctcttata attatgggta ttctacggaa acaatatacc gtggtgaagc taatttactc
    24001 gattgctgcg atgactacct ccgggggaca aaccttatgt aaatactatg gtcctgcggt
    24061 gatgatttgt atgtgataca cagcaacatt tcgagatatt catacggcat ctaatactga
    24121 tttaattctg gttaaaatac agacagataa caagatgaat attcttaatg tttacgttaa
    24181 aaatgtttaa
//
LOCUS       DAZFFG010000072        23753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFFG010000072 DAZFFG010000000
VERSION     DAZFFG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.072"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            142..321
                     /locus_tag="ACHJ7J_003150"
     CDS             142..321
                     /locus_tag="ACHJ7J_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5001343.1"
                     /translation="MATGAKNAKSQMTTVRIPHEVMDDIEQLKEPGESTAGFLVTAAK
                     GEIKRRQRRKAKEQE"
     gene            complement(862..1050)
                     /locus_tag="ACHJ7J_003151"
     CDS             complement(862..1050)
                     /locus_tag="ACHJ7J_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5001344.1"
                     /translation="MLSTDRFIREKECQQLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCEAVH"
     gene            complement(1411..2640)
                     /locus_tag="ACHJ7J_003152"
     CDS             complement(1411..2640)
                     /locus_tag="ACHJ7J_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5001345.1"
                     /translation="MRRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRVARPERIKLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLESYVKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLKHPHWSL
                     HDIRRTFTTMLNDLGVDSHVVEQLTGHQMPGMQRVYNHSRYLDAKRDALDMWTERLGI
                     LAGTHENVTTLPIAREI"
     gene            2889..4010
                     /gene="roxA"
                     /locus_tag="ACHJ7J_003153"
                     /gene_synonym="ycfD"
     CDS             2889..4010
                     /gene="roxA"
                     /locus_tag="ACHJ7J_003153"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO5001346.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4059..5285)
                     /gene="pepT"
                     /locus_tag="ACHJ7J_003154"
     CDS             complement(4059..5285)
                     /gene="pepT"
                     /locus_tag="ACHJ7J_003154"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO5001347.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            5535..6671
                     /gene="potA"
                     /locus_tag="ACHJ7J_003155"
     CDS             5535..6671
                     /gene="potA"
                     /locus_tag="ACHJ7J_003155"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO5001348.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            6655..7512
                     /gene="potB"
                     /locus_tag="ACHJ7J_003156"
     CDS             6655..7512
                     /gene="potB"
                     /locus_tag="ACHJ7J_003156"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO5001349.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            7509..8303
                     /gene="potC"
                     /locus_tag="ACHJ7J_003157"
     CDS             7509..8303
                     /gene="potC"
                     /locus_tag="ACHJ7J_003157"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO5001350.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            8300..9346
                     /gene="potD"
                     /locus_tag="ACHJ7J_003158"
     CDS             8300..9346
                     /gene="potD"
                     /locus_tag="ACHJ7J_003158"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO5001351.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFNNLDPEMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(9502..10323)
                     /gene="cobB"
                     /locus_tag="ACHJ7J_003159"
     CDS             complement(9502..10323)
                     /gene="cobB"
                     /locus_tag="ACHJ7J_003159"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO5001352.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(10339..11250)
                     /gene="nagK"
                     /locus_tag="ACHJ7J_003160"
     CDS             complement(10339..11250)
                     /gene="nagK"
                     /locus_tag="ACHJ7J_003160"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO5001353.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(11279..12523)
                     /gene="lolE"
                     /locus_tag="ACHJ7J_003161"
     CDS             complement(11279..12523)
                     /gene="lolE"
                     /locus_tag="ACHJ7J_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO5001354.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(12523..13224)
                     /gene="lolD"
                     /locus_tag="ACHJ7J_003162"
     CDS             complement(12523..13224)
                     /gene="lolD"
                     /locus_tag="ACHJ7J_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO5001355.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(13217..14416)
                     /gene="lolC"
                     /locus_tag="ACHJ7J_003163"
     CDS             complement(13217..14416)
                     /gene="lolC"
                     /locus_tag="ACHJ7J_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO5001356.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            14678..15751
                     /gene="ycfT"
                     /locus_tag="ACHJ7J_003164"
     CDS             14678..15751
                     /gene="ycfT"
                     /locus_tag="ACHJ7J_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5001357.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVSAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            15879..19325
                     /gene="mfd"
                     /locus_tag="ACHJ7J_003165"
     CDS             15879..19325
                     /gene="mfd"
                     /locus_tag="ACHJ7J_003165"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO5001358.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            19469..20431
                     /gene="ldtC"
                     /locus_tag="ACHJ7J_003166"
     CDS             19469..20431
                     /gene="ldtC"
                     /locus_tag="ACHJ7J_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO5001359.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKNAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(20513..20770)
                     /gene="bhsA"
                     /locus_tag="ACHJ7J_003167"
                     /gene_synonym="ycfR"
     CDS             complement(20513..20770)
                     /gene="bhsA"
                     /locus_tag="ACHJ7J_003167"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO5001360.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21011..21643
                     /gene="comR"
                     /locus_tag="ACHJ7J_003168"
     CDS             21011..21643
                     /gene="comR"
                     /locus_tag="ACHJ7J_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO5001361.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(21705..22244)
                     /gene="ycfJ"
                     /locus_tag="ACHJ7J_003169"
     CDS             complement(21705..22244)
                     /gene="ycfJ"
                     /locus_tag="ACHJ7J_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5001362.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(22471..23751)
                     /gene="ndh"
                     /locus_tag="ACHJ7J_003170"
     CDS             complement(22471..23751)
                     /gene="ndh"
                     /locus_tag="ACHJ7J_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO5001363.1"
                     /translation="MIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLH
                     EVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELLVPE
                     RKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGAN
                     GKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPP
                     RISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKD
                     IGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCAM
                     NNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISL
                     YRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT         5742 a         6236 c         5901 g         5874 t
ORIGIN      
        1 gcgagcactg cggggcgttg ctcttttagg tgtatttcac ctttgctttt taatttactc
       61 actggtgaaa tacacttcaa gccttttttt atcatttttt ttgcgtatac tgacatacac
      121 caatttttag gagatccaga aatggcaact ggtgcaaaga acgcaaaatc acaaatgaca
      181 actgtcagaa tcccacatga agtaatggat gatattgagc aacttaaaga acctggcgaa
      241 agtaccgctg gttttttagt tactgccgca aaaggcgaga tcaaacgccg ccagcgtcgc
      301 aaggccaaag aacaggaata gcccaccagc aagccagcac actgatcaca ttgcccacca
      361 gccgcaaatg tggcattgtt ggtgacgcgt ttgatttcct ttgttctcac tggcgaccat
      421 tttgcggtcg cctttttttt gcctgttatc cggcaattgt ggcgcttcgt cacctggctg
      481 atataatccc actgctcata tttatttttt gtgcagcagg ttaattgttc acaaaggcgc
      541 tccggcaacg gggcgctttt tgtttttatt agtttgtaga aacctgactc agagataatg
      601 ctcatattct gattgccgtc aggggtgtaa gttaaattta ctcccttttt atcatcaaac
      661 atctgcaacg ctctgtcgtt aacaatattg gtttcactcc cgccagtaag gccggaaata
      721 accggaataa tttcggcaaa attttgcaga ttctgttttt caggacgaac gaagccccgc
      781 ccttgttcgg gagaatatcc agtattcatg gttagatctc tgtattagtg gatgggtggc
      841 ggctgtgtgc cgccagcctg attagtgaac tgcctcgcag ctgtccttcc atgccagaac
      901 ttcggataaa gaccagccaa cggaacgacc gccgagctta cgccgtgatg gaaattgtcc
      961 ggctttttcc aggcggtacc gacatgagcg gctcaggcct gtaagttgct gacattcttt
     1021 ttcacgtata aagcgatctg tgcttaacac aattccccct ttgttgttta ttaaagagtc
     1081 attgggtgga ttattgggtt gtgtctgatt gtgtcagggt gtttgtggaa tggcaaacac
     1141 tggcggaggt cgttttacag aagcaggggg aatgagaata aaaactcttt taattcatta
     1201 tgatgcaaag gcataaaatt ttacttttac gcctttttta catattttta agagtgattc
     1261 gccagtgtat aaaaaaacag tacacctata aaaatcaaat ggttatcacg atggctaatt
     1321 tttaacccgt tgttccattt tgttccttgt tgttcgtcgt tgtgtctagt tgtttcacgt
     1381 tgtccgcatc ctgaaaaatc gcaaaaaaaa ttatatttct ctggctattg gcaaggtagt
     1441 tacgttttca tgtgttcccg ccagtatccc taaccgctcc gtccacatat ccagcgcgtc
     1501 acgtttcgca tccagataac gggaatggtt atagactcgt tgcatccctg gcatctgatg
     1561 acctgtaagc tgctccacga cgtgagaatc cacgcctaaa tcgttcagca tcgttgtaaa
     1621 ggtgcgccgg atgtcatgca gtgaccagtg agggtgtttt agcctcctgt gcgctaatct
     1681 gccgtactgc gacacgcttg tttcctgttt cacttccccc agcaataagc ccgtgtgcct
     1741 gttctgctcc accagctgcg tgacgaacgg cagtattgct tccggtatgg gccggaatat
     1801 tgccaccttc gttttgctgt gttctttcgg cacggtccag agcatttcgg taaaatccca
     1861 ctcgctgatc tccgataacc tcagttctac cgtccggcat ccgaaaacaa tcaggaggcg
     1921 gattagtgcg acgtagtaag gggaaaatat ttttttgtcc agtgcctgca ataattcgcc
     1981 aagttctttg gtgcttaaga cacgctcgct tatatccggt tttttcccaa cgtccgccac
     2041 actcatatca tcaagaacgt tgctgattgc atagcgccgc ctccggcaga acttaagcgc
     2101 ctgtttgcac gtctgtagca agaatccggc agtaacaggc gttcgctttg ccacctggtc
     2161 aaaacaggcc agccagtgcc gtagctcgca tttatccagc ggcatagcac caatgtgctg
     2221 tattacgtga ttattaaggc gctttttcag ggcggcataa tccacgcggt tttcctttac
     2281 gtaagactca agccagtagg taagcgcatc gccaaccgtt acgggcttta acgcttcctg
     2341 tacggtgtaa ttcatctcat gacgtggatt tttcccctca gcaagccaag cgcgacactg
     2401 tgcagctttt tccctggcag ctttcagact cagatcagga taatttccca gcttaatccg
     2461 ttccggtcgt gccacccttc ccgttccggc cctgtaggtg aaataccagg ttaaaaggcc
     2521 gctggttgaa tgcctgacgc tcaggtttcc accgtcatta agaaaggctg ttttttgggc
     2581 gggtgtgccg ttgattttcc ttagctgtgt atcactcagt ttgttaagtg ctctgcgcat
     2641 aattttgatt ccggtcatca caattaacta caccattaac tacaccgatc gttgcacaaa
     2701 gcgctacaac gtgaaacaag ctgaaacaag gaaaatagca aatccgttga taatcaaaaa
     2761 cataatgaac aatctgaaac attatgaaac ggcaaaaaac actaattgat aaaatatgtc
     2821 catacttcac gcattacgtt aagcatccgt tataatcggt tgcagatacc agcctgtgga
     2881 tgcttaacat ggaataccaa ctcactctta actggcccga ttttcttgaa cgtcactggc
     2941 agaaacgccc ggtggtgtta aaacgcggct ttaataattt tattgacccg atctctccag
     3001 acgagttggc gggcctggcg atggaaagcg aagtcgacag tcgactggtc agtcaccagg
     3061 atggcaaatg gcaggtcagc cacggtccgt tcgaaagcta cgatcatctc ggtgaaacta
     3121 actggtcatt gttagtgcaa gcagtgaatc actggcatga gccgaccgcc gcgctgatgc
     3181 gaccgttccg tgaactacca gactggcgta ttgatgatct gatgatctct ttttctgtac
     3241 ctggcggcgg cgtcggcccg catctcgatc agtacgacgt gtttatcatt cagggtaccg
     3301 gacgtcgtcg ctggcgagtg ggcgaaaaac tgcaaatgaa acagcactgc ccacatccgg
     3361 atctgttaca ggtcgatccg ttcgaagcca tcatcgatga agagctggag cctggtgata
     3421 ttctttatat tccgccagga ttcccgcatg aaggctacgc gctggaaaat gcgatgaact
     3481 attccgtggg ttttcgcgcg ccaaatacgc gggaattaat tagcggattt gccgattatg
     3541 tgctgcaacg tgaactgggc ggcaactact acagcgatcc ggatgttcca cctcgcgctc
     3601 atcctgcgga cgttctgccg caagagatgg ataaactgcg tgagatgatg ctcgaattga
     3661 tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt tatatcccag tcacgtcatg
     3721 aactggatat cgcgccgccg gagccgcctt atcagccgga tgaaatctac gatgcgctga
     3781 aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt attgcgcatt ggcgacgacg
     3841 tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc ggcactggat gcactcgcca
     3901 gcaacattgc gctgactgcg gagaattttg gcgatgcgct ggaagatccg tcattcctcg
     3961 cgatgctcgc ggcgctggtc aatagcgggt attggttctt cgaagggtaa gtttggattt
     4021 aagccggatg cggcatcaca tgccgcatcc cttttcgctt acttccgttg cgccgttaac
     4081 tcggcaatac ggacgatcac ctgcaccgct ttttccatac cttccagagt cacaaactca
     4141 tgcttaccat gatagttgta accgccagtg aacaggttcg ggcacggtaa tcccataaac
     4201 gacaactgcg cgccgtctgt accaccgcgg atcggtttca gttccggttc aatatcgcag
     4261 tcacgcatcg cctgctgggc gatatcgaga atatgcggat gctcaaccac tttctcgcgc
     4321 atattgtagt aactgtcttc aatcaccaat tcaatgtagc aatcaggatg taaccctttg
     4381 cccacttttt tggcgatctc catcatttta cgtttacgcg cttcaaactg tttacggtcg
     4441 aaatcacgga tgatgtagtg catatcggcc cgttcaacgg tgcctttcat gctcgccagg
     4501 tgatagaaac cttcatagcc ttctgtcatt tccgggcttt catccgccgg aacttccgca
     4561 tgaatacgtg ccgccagcga cagcgcattt accatcactc ctttcgccgt acccgggtga
     4621 acattgttac cgacaatttt gatattgacc gacgcggcgt tgaagttttc aaactccagt
     4681 tcgcctacgc caccaccatc aacagtgtaa gcccagcggg catcgaaggc gtcaacatca
     4741 aaatgtttcg cccctttgcc cacttcttca tccggggtaa aggcgacgcg aatatcacca
     4801 tgcggaatgt ttttctgttg caataccgcc agcgcggtca tgatttctgc aataccggct
     4861 ttgtcatcgg cacctaacaa ggttttacca tcggtagtaa tcagcgtctg acccagtagc
     4921 tgatgcagca ccgggaacat aaccggtgat aaaacttcat cgccgatacc cagcgcaata
     4981 tcgccaccgc gatagttttc aacaatttgc ggattcacat ttttgccgct gcaatccggt
     5041 gaggtatcca catgagaaat aaagccaatc gccgggatat cgccagggac gttagccggt
     5101 aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat tgataagccc catctcttcg
     5161 agctgctctt tcagcagatg caataacttc cattggcctt ccgtgctggg aacctgtctc
     5221 acccctgctt ttgattgggt atccagagac acgtagttca aaaatcgctc aagtagttta
     5281 tccatgtagt caccctcact ttttgtgaca acattattaa gaagcaagaa aagacaaata
     5341 ttgcgtcagg tcacttttac ccctgcaagc gggaatattt atcagcatta ccttaatgaa
     5401 tataaagcta agccagtaat tcacaacaaa gattggcgat tcaagcggtt tgccgtagaa
     5461 ttaccgccct cattaagagt caccaaggtg gttaaccaca aaccccgcat cggtaagcca
     5521 tccgttgcgt ttacatggga cagagtaaaa aattgaataa acaaccgagt tcgctttcac
     5581 cgctggtgca attggcggga attcgcaaat gctttgatgg taaagaggtc attccccagc
     5641 tggatctgac tatcaacaat ggcgagttcc tcacgctgct tggcccttct ggctgcggta
     5701 aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt tgattccgga cgcatcatgt
     5761 tggataacga ggacatcacc cacgttccgg cggaaaaccg ctatgtgaac actgttttcc
     5821 aaagctacgc acttttcccc cacatgaccg tgttcgaaaa tgtggccttt gggttgcgca
     5881 tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat ggaagccctg cggatggtgc
     5941 agctggaaac cttcgctcaa cgtaaaccgc atcagctctc tggtggtcaa cagcaacgcg
     6001 tcgccattgc tcgcgcggtg gttaacaagc ctcgtctgtt gttgctggat gagtcgctct
     6061 cagcgctgga ttacaaactg cgcaagcaaa tgcagaacga gctgaaagcg ttacagcgta
     6121 agcttggcat tacattcgtc ttcgtgactc acgaccagga agaagcactc accatgtcgg
     6181 acaggattgt ggtgatgcgc gatggtcgca ttgagcaaga cggcacgccg cgtgaaatct
     6241 acgaagagcc gaaaaacctg tttgttgccg ggttcattgg cgaaatcaat atgtttaacg
     6301 ccactgtcat cgaacgtctc gacgagcagc gcgtacgcgc caacgttgaa ggccgcgaat
     6361 gtaatatcta cgttaacttc gccgttgaac cggggcaaaa actgcatgtt ctgctgcgcc
     6421 cggaagactt acgtgttgaa gagattaacg acgacaacca cgctgaaggg ctgattggtt
     6481 acgttcgcga gcgtaactac aaaggcatga cgctggagtc ggttgttgaa ctggaaaatg
     6541 gcaagatggt gatggtcagc gaattcttca atgaagacga tcctgacttt gaccactctc
     6601 tcgaccaaaa aatggccatt aattgggtag aaagctggga ggtcgtactg gctgatgaag
     6661 aacacaagta agttccagaa tgtagtgatt gtcactattg tcggttggct tgtgttgttt
     6721 gtctttctgc ccaacctgat gatcattggc accagctttt tgacccgcga cgacgccagt
     6781 ttcgtcaaaa tggtctttac gctggataac tacacacgtc tgctcgatcc gctctatttt
     6841 gaagtgctat tgcactcgct gaatatggcg ctgatcgcca ccctcgcctg cctggtgctg
     6901 ggctatccgt ttgcctggtt tctggcgaag ctgccacaca aggtgcgtcc gctgctgctg
     6961 tttctgctga ttgttccctt ctggaccaac tcactgattc gcatctacgg gctgaaaatt
     7021 ttcctcagca ccaaaggcta cctcaacgag tttttgctct ggctgggcgt tatcgacaca
     7081 ccaatccgta tcatgttcac gcccagtgcg gtgattatcg gtctggttta cattctgcta
     7141 ccgtttatgg tgatgccgct gtactccagt atcgaaaaac tggataagcc gttgcttgag
     7201 gcggcgcgcg atctcggtgc cagcaagtta cagactttta tccgcatcat tattccactg
     7261 acgatgccgg ggattattgc cggatgtctg ctggtgatgc tgccagcgat gggcctgttc
     7321 tatgtgtccg acctgatggg cggtgcgaaa aacctgctga tcggtaacgt catcaaggta
     7381 cagttcctca atattcgtga ctggccgttt ggtgcagcca ccagcattac gctgactatc
     7441 gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc gtctgctgaa taaaaaggtg
     7501 gaactcgaat gatcggtcga ctgcttcgcg gcggttttat gaccgctatc tacgcgtacc
     7561 tgtatatccc aatcattatt ttgattgtga actcctttaa cagctcgcgc tttggcatca
     7621 actggcaggg ttttaccact aaatggtata gcttgctgat gaacaacgac agcctgttac
     7681 aggcagcgca gcattcacta acaatggcgg tgttttcggc gacgtttgct acgcttatcg
     7741 gttcactgac ggcagttgca ctgtaccgtt atcgctttcg tggtaagccg ttcgttagcg
     7801 gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat ggcaatttcg ctgctggtgc
     7861 tgtttatgct gctgggtatt cagcttggct tctggtcgct gctgttctcg catatcacct
     7921 tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct gaaaggtttt gacgtgcgaa
     7981 tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac cattctgcga aaaatcattc
     8041 tgccactggc aatgccagcg gtggcggcgg gctgggtgtt aagctttacc ctgtcgatgg
     8101 acgatgtggt ggtttcttcg ttcgtcaccg ggccgagtta tgaaattctg ccgttaaaaa
     8161 tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa cgcgctggca accatattac
     8221 tggtgctgtc gctggtgatg gttattgcca gccagcttat tgctcgtgat aaaacgaaag
     8281 gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct
     8341 ggcactgggc atgagcgccg ctcacgccga tgacaacaat acgctgtatt tctacaactg
     8401 gaccgagtac gtgccgccag gactgcttga acagttcacc aaagaaaccg gtattaaggt
     8461 tatctattcg acttacgagt cgaacgaaac catgtacgcc aagctgaaaa cgtacaaaga
     8521 cggtgcctac gacctggtgg ttccttcaac ctattacgtc gataaaatgc gtaaagaagg
     8581 gatgatccag aagatcgaca agtcgaagtt aacgaacttc aacaacctcg atccagaaat
     8641 gctcaataag ccgtttgacc cgaataacga ttactccatt ccgtatatct ggggtgcgac
     8701 ggcgattggc gttaacggtg atgcggtgga tccgaaatct gtcaccagct gggccgattt
     8761 gtggaagcct gagtacaaag gcagcctgct gctgaccgac gatgcccgtg aagtgttcca
     8821 gatggcgctg cgtaagctgg gctactccgg taacaccacc gatccgaaag agattgaagc
     8881 tgcatataac gagctgaaaa aactgatgcc aaacgtggca gcctttaact ccgataaccc
     8941 ggctaacccg tacatggaag gcgaagttaa cctcggcatg atctggaacg gttctgcttt
     9001 cgttgcacgc caggcgggta cgcctattga cgtggtgtgg ccgaaagaag gcggcatatt
     9061 ctggatggac agcctggcga tcccggcaaa tgccaaaaac aaagaaggcg cgctgaaatt
     9121 gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt gctgaaacta tcggttatcc
     9181 aacgccaaac cttgcggcgc gtaagctgtt aagtccagaa gtggcgaacg ataagacgct
     9241 ctacccggat gctgaaacca ttaaaaatgg cgaatggcag aatgacgttg gcgcagccag
     9301 cagcatttat gaagagtatt atcagaagct gaaagcagga cgttaattca gcaagcgggc
     9361 ggtggtatta ccgcccgttt tttagttagc aaggatgtgg ctaatgagtc aggacagtaa
     9421 agttttatcc cgctctttcg tcggtattgt ggtccgcttg taggcctgat aagcgtagcg
     9481 catcaggcaa tgcttcctgc tttataatcc cttcagcaac ttttcaacaa attctggcac
     9541 cacctggctt gccgggccgt aatatttctc ggcaaattca ttaccgacct gacttggttc
     9601 aagattcagc tccacggtgt gcgcaccatg cagtttcgct tcgtgaacaa acccagccgc
     9661 cggataaaca tgccccgaag taccaatggc aatgaaaata tcggccatcg acaacgccat
     9721 ataaatttca tccatgccga gtggcatttc gccaaaccac actacgtgcg ggcgcaaggg
     9781 tgccggaaac tggcagcaat ggcatttatc ttctggagta acgtctccgg tccagtcgag
     9841 aacctgacca ctttgtgaac aacgaacttt cagcagttcc ccatgcatat gaatcacatt
     9901 ggtattacct gcgcgttcat gcaggttgtc tatattctgc gtcaccagca aaaagcgatc
     9961 gccgagagca tcttgcagtt tagccagcgc aagatgcgcg gcgttaggct gaatttctgg
    10021 ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc accagttcag gatcgcgatc
    10081 gaaaccttcc ggcgttgcca catcttcaac ccgatgttct tcccacaggc catctgcggc
    10141 gcgaaaggta cgaatacctg attccgcaga aattcctgcc cctgtcagta cgagtactct
    10201 tggtttttcc attgcttccg gcaccacttt atctctgaaa aaaatacgct gacgcaaacg
    10261 ctcgcgcagg cggcgtttat ttttacgaaa acgacttaac cgatgacccc gacgcgacag
    10321 catagcaacc tctgttgttt aatcggttag atgtaggaag gccgcaccac gcattcctcc
    10381 cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga gctacaggta agagatgacg
    10441 aggcagcctg tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc caccaccaat
    10501 gacgaccagg tcagggtcaa caatggtcag gatatttccc agacaaaccg ctaataaatc
    10561 cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct tgatcataaa gcgcaataat
    10621 ttcgggagcc tgcaacggtt gatgataata gtgttgatac agccacgcaa aaccgcgacc
    10681 agacagataa ttttcaatgc agccatgctg accacagccg cagcggcgta acgggaaatc
    10741 caaccccatc atggttaacg catcaaccgg cagacgcata tggccaaact cgccggtaat
    10801 gtagcttttc ccggtaatcg gtttgccgtt gaaaatcagc ccgccgccaa cgccagtgcc
    10861 gagaatcaac cccatcacca gtggatattg agtaaattca tcatcccagg cttctgaaag
    10921 ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga tcaagacgtg cgctcaggtc
    10981 ggcacgcagc ggtttaccgc tggcagcggg gacattggcg gcatacagcg taccatcttc
    11041 tgtttccggc attcccggaa taccgatgcc gacagaacct ctacagccaa aacgttgatc
    11101 agcttcggct accagctcgc acactgcatc taaaaatgcg tcatagctat cacgcggtgt
    11161 cggcacccgc ttttcccact gcaactgccg accgctatca aacacgccaa gcgcaatttt
    11221 tgttccacca atatcaaacc cgtaatacat tgccgctcct tgttttaatg tactgccttt
    11281 actggccgct aaggactcgc gcagggtcaa tattactggc gcgccgcgcc ggataccaac
    11341 ttgccaaaag actcagcaac aatgctgtga ccagtacgta gaagacgtcc agccagtgca
    11401 attccgatgg caggaagtca ataaaataga tatcgctgga gaggaactga tgaccgatca
    11461 gcttttcaat ccgctcaata atcggggtaa gttgcagtga aacaactacg ccgataatca
    11521 ccccacacag gctgccgaac agccctgcca gcaatccata ccagacaaag atggcgcgaa
    11581 ttaaaccatc tttcgccccc agcgttctta atactgcgat atcgccactc ttgtctttca
    11641 ccgccatcac taaggtggag acgatgttga aacaggccac gccaatcacc agtaccatcg
    11701 ccagatacat aatggcgcgg atcatctgaa tatcgcgata catatagccg taagtaccaa
    11761 tccagctttt aatataaaca tagctgttgg tcacttcacc cgcatcgcgt accagcttat
    11821 tggcgttgaa aacatccgtc attttaaggg caatacctga cacgctggaa cccatatcaa
    11881 gatattgttg ggcatccgcc agcgggatca tggcaaaact gtgatcgagt tgaccactca
    11941 attgcaaaat accggcaacg tgcaaacgca ctcgttttgg ctgcatcagt ttatgctcag
    12001 gattcgagtt ggggatcata atcgacaccc aatcgccctg cttcactttc agcgcatccg
    12061 ccacgccttt gccgatgata atttgctgtt cgcccgcttt aaaattgcgc caggcatccc
    12121 cctgaacaaa cgagggtaat gcgctcagac gctgttcctg ttgcgggtta acgcccttca
    12181 cctggattgc gcgcagattc gctccacttt ccaccagccc ggtgaaattg atatacggcg
    12241 cagcagcggc aatacctggc actttttgca cgttatccag tgcttcctgc cagttagtcc
    12301 acggttgatc caccgcctct atttcgccat gcggcaccac cgccagaatg cggttattca
    12361 gttcgcgttc aaagccgttc atcgcgctta agccgacgat caataccgcc acgccaaggg
    12421 caatgccaat ggtagaaatg acggagatca gcgacaccat gccgccgcgt cgccgtccgc
    12481 gactaaaacg caggccaatt aataacgata aaggcatcgc cattactccg cccccatcag
    12541 gctcagttct gccgtcagac gaccatcacg catctccagt tggcgactca tacgtttcgc
    12601 cagttgcagg tcgtgagtaa ccaccaggaa ggcagtaccc tgcaaacgat tcaactcccc
    12661 gagcaactgg aagatgctgt ctgcgttacg agcatcgagg ttaccggtag gttcatccgc
    12721 cagtaccaga cgcgggttat tgaccagcgc acgggcaatc gccacacgct ggcgttcgcc
    12781 gccagaaagt tcagatgggc ggtgattcgc acgatgctcc agccccaccg cttttagcat
    12841 ttccagcgca cggctgttga tttcagcggg ctttttcttg ccaatcagca gcggcatagc
    12901 cacgttttcc agggcggtaa aatccggcag caggtggtga aactgataaa taaagcccag
    12961 cttctggttg cgcagttctg ctttcgcagc cgacgacagt ttgctcattg gctgaccatt
    13021 aaaaatcaca tcgccggagg ttggcgtatc cagcccgccc agcaggtgca gcaaggtact
    13081 tttaccggaa ccagagctac cgacgatcgc catcatttca ccttcgccga cgctgaaact
    13141 gacattgtgc agcacatcgg tttgcacact gccttcctga tagcgtttgc acaggttgtc
    13201 gcattgcaac aggatcttat tcataacgta aagcctcagc gggttgtgtg gcggcagcgc
    13261 gccatgaagg gtaaagcgta gacagcagcg cgatagccat cgccaccagc gcaataacaa
    13321 tgacctgtaa aggttcgata gcgaccggca gcgccgcgcc atcaagcagg acgccgatta
    13381 tcggcatcag attatttaac tggctggcaa gcagggcgcc aagcgccgct ccgaggatcg
    13441 caccgataat cccggcgctg gccccttgca ccataaagac catcatgatt tgtcgcggag
    13501 ttaagccttg cgtttgcagg atcgctactt cgccctgctt ctccattacc atcagcccta
    13561 gtgaggtaat aatgttaaac gccgcaacgg cgacaatcag gctcagcagt aaacccatca
    13621 tatttttttc catgcgtacg gcctggaaca actcgccttt acgatcacgc cagtcctgcc
    13681 atttgctgcc ttcaggcagt ttttgctgac ttaatgagtc gactttcagc ggctcatcca
    13741 gccacaaacg ccagccggta atattgcctg ccggataacg catcagacgc gaggcatcct
    13801 caatattcac cagcatttca ttgccatcga cttcactgtt ggcggcgaaa gtgccaatca
    13861 cattgaacag gcgctgactt ggaatacgcc ccatcggcgt gaactggctg gcagatggta
    13921 ccatcatgcg gatttgatca ccgcgattga cgcccaactg tgaggcaagc tgttcaccga
    13981 ggatgacatt atatttcccc ggctcgaggt cagtttgttt cacattgacc agatacggcg
    14041 ttagtggatc tttttgcgcc ggatcgatac ccagcatcac cccgaccgcc acgctgcgcg
    14101 cgctttgcag taccacatca ccggtagtaa taggtgcgac gcgattaacg ccgtccagtt
    14161 tgactgccgt ttccgggagt tgctgcgggt taagagagcc atgctcagaa gagagaattg
    14221 cctgtggcat caggccaagg atgttgtttt gcagctcgcg ctcaaagccg ttcatcactg
    14281 acaatactgt gaccagcgcc atcaccccga gggtaatgcc gatggtagaa agccaggaga
    14341 cgaaacgacc gaagcgatcc gctgcacgcc cacgcatgta acgcaggcca ataaatagag
    14401 cgacaggttg gtacatgaaa tccgtctggt tgctgtagca aagacacgag tatataagcg
    14461 aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc agggctaatc tgaaaagccc
    14521 ccgtaaatca atcggctaaa ttgccatgtt ttatcatcaa tcttgccgcc tgatcttaat
    14581 ctgaaaaatc atccaataca gacagatacc cattacacct cgggtcataa cccgcaggat
    14641 caaccgcatg taaattaaaa aagagcgtgt tgtcctgatg aaacaaaaag agctatggat
    14701 taaccagatc aaagggttat gtatttgtct ggtggtgatt tatcactcgg tcattacctt
    14761 ttatccgcat atgaccactt ttcagcatcc gttatcggaa gtcctgagca aatgctggat
    14821 ctatttcaat ctttaccttg caccctttcg tatgccggtt tttttcttta tatccggcta
    14881 tttgattcgc cgctatatcg acagcgtacc gtggggaaat tgtctcgata aacggatctg
    14941 gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag tggctggcgt taagtgcgct
    15001 gaaccagtgg ctggcacctg agcgcgattt aagcaatgcc tccaatgccg cttatgccga
    15061 ttccaccggt gagttcctgc acgggatgat caccgccagc accagcttgt ggtatctgta
    15121 tgcgttaatt gtctatttcg tggtatgtaa aatttttagc cgtctggcgc tgccactatt
    15181 cgccttgttt gtactgctga gtgtggcggt taatttcgtt cccacgccgt ggtggggaat
    15241 gaacagtgtg atccgcaatt tgccttatta cagccttggc gcatggtttg gcgcaacaat
    15301 aatgacctgt gttaaagagg tgccgttgcg ccgccatctg ctgatggctt ctttgctgac
    15361 cgttctggcg gtcagtgcct ggttgtttac tatctcgctg ctgttgtcgc tggtatcgat
    15421 tgtggtgatc atgaagctat tttatcaata cgagcaacgt ttcggtatgc gctccaccag
    15481 cctgctgaat gtgattggct ccaacaccat tgctatctac accacccatc gcattctggt
    15541 tgaaatattc agcttaactc tgcttgcgca aatgaacgca gcacgctggt cgccgcaagt
    15601 cgaactgaca ctcctgctgg tttatccctt tgtcagtttg ttcatctgta ctgttgcggg
    15661 cttgctggta agaaaacttt cacagcgtgc attcagcgat ctgttgttct ccccgccttc
    15721 actgcccgct gccgtcagtt actcccgcta agccatcgcc ccagccgggg cgattcaatt
    15781 tgctaaacca tgtcgattac ggataatgaa cagcattgct tatcagagat atgcccccat
    15841 atgttgaggc atatcctaac gagaatctga caaccgttat gcctgaacaa tatcgttata
    15901 cgctgcccgt caaagcgggt gagcagcgtc tgctgggcga gttaaccggc gctgcctgcg
    15961 cgacgctggt ggcggaaatt gccgaacgtc acgccggtcc ggtggtactc attgcaccag
    16021 atatgcaaaa tgccctgcgt ttgcatgatg aaatcagcca gtttaccgac cagatggtga
    16081 tgaatctggc ggactgggaa accctccctt acgacagttt ttcgcctcat caggacatta
    16141 tctcctcgcg cctttccacc ctttaccagc taccgacaat gcagcgtggc gtactgattg
    16201 ttccggtgaa tacacttatg cagcgcgttt gcccgcacag ttttctccac ggtcatgcgc
    16261 tggtgatgaa aaaaggtcag cgcctgtcac gagatgcatt acgaacccaa ctggacagcg
    16321 ccggttatcg ccatgttgac caggtgatgg agcacggcga atacgccacg cgcggcgcgt
    16381 tgctggatct cttcccgatg gggagtgagc tgccttatcg tcttgatttc tttgatgatg
    16441 aaatcgacag cctgcgggtg tttgacgtcg acagccagcg cacgctggag gaagtagaag
    16501 cgatcaatct gctgcccgcg cacgaatttc cgaccgataa agcggcaatt gaactgttcc
    16561 gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc ggaacatatt taccagcaag
    16621 tgagtaaagg cacattacct gccgggatcg agtactggca gccattgttc ttcagcgaac
    16681 cactgccgcc gctgttcagt tatttccctg ccaatacctt gctggtgaat actggcgatc
    16741 tggaaaacag tgccgaacgt ttccaggctg acacgctggc gcgttttgag aatcgcggcg
    16801 tcgatccgat gcgcccactg ttgccaccac aatcgctctg gctgcgggtg gacgagctct
    16861 tctcagagct gaaaaactgg ccccgagtgc agctaaaaac tgaacattta ccgacaaaag
    16921 ccgcgaatgc caatttaggt ttccagaaac tgccagacct ggccgttcag gcacaacaaa
    16981 aagcgccgct ggatgcgctg cgtaagttcc tcgagacttt cgacggtccg gtggtgttct
    17041 cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact gctcgcacga attaaaattg
    17101 ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg tgggcgttat ctgatgattg
    17161 gcgctgccga acatggtttt gtcgataagg tgcgtaacct ggcgctgatt tgcgaaagcg
    17221 atctgctcgg tgaacgcgtt gcccgccgtc gtcaggattc tcgccgcacc atcaaccccg
    17281 atacactgat ccgcaacctc gcggagctgc atattggcca accggtggtc catctggagc
    17341 acggcgtcgg tcgctatgcc ggaatgacca cgctggaagc tggcggcatt actggcgagt
    17401 acctgatgct cacctatgcc aacgacgcca aactgtatgt tccggtgtcg tcactgcatc
    17461 tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc gctgcataaa cttggcggcg
    17521 atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt gcgtgatgtg gcggcggaat
    17581 tgctggatat ctacgcgcaa cgcgccgcca aagagggctt cgcgtttaaa cacgatcgtg
    17641 agcagtatca gttgttctgc gacagcttcc cgtttgaaac cacgccggat caggcgcagg
    17701 ccattaatgc ggtacttagc gacatgtgtc agccgctggc aatggatcgt ctggtgtgcg
    17761 gcgatgttgg ctttggtaaa acagaagtgg caatgcgcgc cgctttcctg gcagtagata
    17821 accacaagca ggtggcggtg ctggtgccta ccacccttct cgcgcagcag cattacgaca
    17881 acttccgcga ccgtttcgcc aactggccgg tccgtatcga aatgatctcc cgtttccgca
    17941 gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga agggaaaatc gatattctga
    18001 tcggtacgca caaactgctg caaagtgacg tcaagtttaa agatttaggc ctgctgattg
    18061 tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg cattaaagcg atgcgcgcga
    18121 acgtggatat tctgacgctt actgcaacgc cgatcccacg tacgctgaat atggcaatga
    18181 gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc ccgtcgtctg gcagttaaaa
    18241 cctttgtccg tgagtatgac agcctggtgg tccgggaggc gatcctgcgt gaaattttgc
    18301 gcggggggca ggtttattat ctctacaatg atgtggaaaa cattcagaaa gccgccgaac
    18361 ggctggcaga actggtgcca gaagcgcgga tcgccatcgg tcacgggcag atgcgcgagc
    18421 gcgaactgga acgggtgatg aatgatttcc atcatcaacg tttcaacgtg ctggtttgta
    18481 caaccattat cgaaaccggg atcgacatcc cgacagccaa caccattatc attgaacgcg
    18541 cggatcactt cggtctggcg cagctgcacc agttacgcgg tcgcgtcgga cgttcgcatc
    18601 atcaggcata tgcatggttg ctgacaccgc atccaaaagc gatgactacc gatgcacaaa
    18661 aacgtcttga agcgattgcc tcgctggaag atctcggggc aggttttgcg ctggcaacgc
    18721 acgatctgga gattcgcggc gcgggtgaac tgcttggcga agaacaaagc ggctcaatgg
    18781 aaaccatcgg tttctcgctg tatatggagt tgctggaaaa cgccgtcgat gcactgaaag
    18841 ccggacgcga gccgtcgctg gaagatctca ccagccagca aacagaagtc gagctgcgga
    18901 tgccgtcgct attgccagat gatttcatcc ctgacgtgaa cacgcgtctg tcgttctaca
    18961 aacgtattgc cagcgccaaa acggaaaacg aactggaaga gatcaaagtc gagcttatcg
    19021 atcgcttcgg cctgctgccg gatccggcgc gtaccctgct ggatattgcc cgtctgcgcc
    19081 agcaagcgca gaaactgggg atcaggaagc tggaaggtaa tgagaaaggc ggggtgatcg
    19141 aatttgccga gaagaatcac gttaatccgg cctggttgat tggtttgctg caaaaacagc
    19201 cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt tattcaggat ttgagtgagc
    19261 ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga actggaagag aacgcgatcg
    19321 cttaatggta aatcgggggc gtttctgcgc ccccatctgc aacatttaca aattctttgc
    19381 acttccctgc actatccgac actgtcacca tccataattc aggcttatct gtttattaca
    19441 ataaccttat atttattatg gatttttggt gatgatcaaa acgcgttttt ctcgctggct
    19501 aacgtttttt acgttcgccg ctgccgtggc gctggcgcta ccggcaaaag ccaacacctg
    19561 gccgctgccg ccagcgggca gtcgtctggt tggcgaaaac aaatttcatg tggtggaaaa
    19621 tgacggtggt tctctggaag ccatcgccaa aaaatataac gtcggctttc tcgctctgtt
    19681 acaggctaac cccggcgttg atccttacgt accgcgcgcg ggcagcgtgt taacgatccc
    19741 gttgcaaacc ctacttccag acgcgccgcg tgaaggcatt gtgatcaaca ttgctgagct
    19801 gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc gtgtatccaa taggtattgg
    19861 tcagttaggt ggtgacacgc tgacaccgac aatggtgacc accgtttcag acaaacgtgc
    19921 aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt tataaagcac agggaattga
    19981 gttgcctgcg gtagtgccgg ctggaccgga taacccaatg ggccatcatg cgattcgtct
    20041 ggcggcctat ggcggcgttt atttgcttca tggtacgaac gccgatttcg gcattggcat
    20101 gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat atcaaaacac tctttagcca
    20161 ggtcacccca ggcaccaaag tgaatatcat caacactccg ataaaagtct ctgccgaacc
    20221 aaacggtgcg cgtctggttg aagtacatca gccgctgtca gagaagattg atgacgatcc
    20281 gcagctgctg ccaattacgc tgaatagcgc aatgcaatca tttaaaaatg cagcacaaac
    20341 tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc gggatgccgg tggatgtccg
    20401 ccgtcatcaa gtgagcccac aaactctgta aatcctggat aaaaaaatcc ccgcgacacg
    20461 gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat gagggttaat gcttatttat
    20521 aaattactgc tgttccatgg agagtattcg gaccggttac agaagtaata cggaaagatt
    20581 ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc cagcgatccc agatttgtcc
    20641 ccgcgttagc actgattgta ccgacttttt gttggccttc tggcgttgac tgaacttcga
    20701 cagccgcaaa gctggcaaat gacatggagc ttaaaatcgc cgcagcgatg agggttttta
    20761 cgtttttcat aatagtggcc ttatgcagat gaatgacgtc gcaagcaatt acttaacgat
    20821 cgataactaa atgatagatg tgatcttgat cacatacaag atatttttta taacaatcat
    20881 taattaattt aaaaatcttt aaatttcagt taaatagaaa gcaattattt ttatgggatc
    20941 tggactggtg aacggaggtg attattttta taataacctt tcattcacag tcaccaggta
    21001 taacggcatc atggcaactg actcaacaca atgtgtaaaa aaaagccgtg gccgcccaaa
    21061 agtgttcgac agggatgccg cgcttgataa ggccatgaaa ttgttctggc aacacggtta
    21121 tgaagcgact tctcttgcgg acctcgtcga agcgaccgga gccaaagcgc ccacgctata
    21181 cgcggaattt accaacaaag aggggttatt tcgtgccgtt ctcgaccgct atatcgatcg
    21241 ttttgccgct aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct
    21301 ggctgactat tttgctgcca tcgccaactg ctttaccagc aaagacaccc cggcaggctg
    21361 cttcatgatc aacaactgca ccaccctctc cccagattca ggagatatcg ccaatacgtt
    21421 gaaatcacgc catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc
    21481 gcgcggggaa atcccgaccc actgtgacgt gacacatctg gcagaattcc ttaattgcat
    21541 tattcagggg atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac tgatgcagat
    21601 tgccggaacg actttgcgtt tatggcccga actggtgaaa taagtcgcaa aaaaagcccc
    21661 tcagcctgag cgaatgaggg gccaaattgc agagtacagc cttgttatac tttgttattc
    21721 aaaatcagtt gcccgttact atctagcggg atctgcgtac ccggatcgcg gtccatgcgg
    21781 attttgccct gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac
    21841 ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga
    21901 gagccctgga tctggttacc ggcatatcca ccacccagcg cccccacaac agtggcgaca
    21961 tctttaccac gaccaccacc aaactgatgc cctatcacgc cgccagcaac agcgccgagc
    22021 accgacccgg taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtgacgttg
    22081 cgacactcct gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga
    22141 gcgtattgcg ggccacgttc aaacacgttc agactggcca ctgccgctac gccaagcgca
    22201 gctgcgacac caatcccgat acccgccaac attgatttat tcacgggaat acctccttct
    22261 ttgcagttat gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa
    22321 agcgcgggaa tggctggaaa tacttaagga ttcggaggag tctgaaggaa tgcctgatgc
    22381 gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg cggatgaggc
    22441 gtttatgcca catccgccag tgtacgtcga ttaatgcaac ttcaaacgcg gacggataac
    22501 gcggttaata ctccccacca gcatcattaa tccggtttta aagtaaccat gcagcgcaat
    22561 ctgatgcatt cggtatagcg agatatatac aaagcgcgca attcgtcctt caatcatcat
    22621 tgagccgcgc gtcaggttac ccatcaggct accaacggtg gagaagttcg acagcgatac
    22681 cagtgaacca tgatctttat actgataatt tttcagcggc ttaccgttca tctgcgccag
    22741 aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc tgagcacgcg gcggaacaaa
    22801 gcccccttcc ggacgcgggc atgacgcgca gtcgccaata gcgtaaatat ctggatcgcg
    22861 ggtggtttgc agcgtcggtt ccaccaccag ctggttgata cggttagttt caagaccacc
    22921 gatatctttc aggaagtctg gcgctttgat cccggctgcc cataccatca gatcagcctc
    22981 aatatattcg ccatctttag tgtgcaggcc gccttcatca gcactggtga ccatggtttg
    23041 cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg gccgcagcag agatacgtgg
    23101 cggtaatgca ggcaaaatac gttctcccgc ttctaccagc gttacgttca gggcttcgtt
    23161 ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgacc gcgttgtgca attcagcgga
    23221 gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg ttcactttgc catttgcgcc
    23281 caggttggcg gagtatttca ggaagagatt cagcatctcc tggtgaaagc gacgcgcctg
    23341 gtgcgggtta tcgaggaaaa tgcagttctc tttgacacct ggcgtattga aatcgttaga
    23401 ggtgctaccc agcgccatta ccagggtgtc ataggcgatt ttacgctccg gaaccagcag
    23461 ttcacctttc tcatcgcgca gttctgcaat agtgattgtt ttcgcttcac gatcaatatc
    23521 aatgacggaa cccagctgga actggaaacc atgattgcgc gcatgggcca gatagctcaa
    23581 cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg tgcagcagcg gtttccacag
    23641 gtggctgtgg ttacgatcga ccagcgtaat tttggctttt ttcttgcgtc ccagcttatg
    23701 ccccagctgt gttgccattt ccagcccacc agcaccgccg ccgacaatca caa
//
LOCUS       DAZFFG010000073        23654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFFG010000073 DAZFFG010000000
VERSION     DAZFFG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.073"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            2..1630
                     /gene="yqiK"
                     /locus_tag="ACHJ7J_003171"
     CDS             2..1630
                     /gene="yqiK"
                     /locus_tag="ACHJ7J_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO5001364.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEAERRREAEQTRI
                     LAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDASAGSVSGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1869..1928
                     /locus_tag="ACHJ7J_003172"
     CDS             1869..1928
                     /locus_tag="ACHJ7J_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5001365.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2244..2303
                     /gene="ibsE"
                     /locus_tag="ACHJ7J_003173"
     CDS             2244..2303
                     /gene="ibsE"
                     /locus_tag="ACHJ7J_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO5001366.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(2423..3856)
                     /gene="hldE"
                     /locus_tag="ACHJ7J_003174"
     CDS             complement(2423..3856)
                     /gene="hldE"
                     /locus_tag="ACHJ7J_003174"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO5001367.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3904..6744)
                     /gene="glnE"
                     /locus_tag="ACHJ7J_003175"
     CDS             complement(3904..6744)
                     /gene="glnE"
                     /locus_tag="ACHJ7J_003175"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO5001368.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTVALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSENDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMALPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6767..8068)
                     /gene="ygiF"
                     /locus_tag="ACHJ7J_003176"
     CDS             complement(6767..8068)
                     /gene="ygiF"
                     /locus_tag="ACHJ7J_003176"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO5001369.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8310..8930
                     /gene="ygiM"
                     /locus_tag="ACHJ7J_003177"
     CDS             8310..8930
                     /gene="ygiM"
                     /locus_tag="ACHJ7J_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO5001370.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8994..10232
                     /gene="cca"
                     /locus_tag="ACHJ7J_003178"
     CDS             8994..10232
                     /gene="cca"
                     /locus_tag="ACHJ7J_003178"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO5001371.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(10413..11234)
                     /gene="bacA"
                     /locus_tag="ACHJ7J_003179"
     CDS             complement(10413..11234)
                     /gene="bacA"
                     /locus_tag="ACHJ7J_003179"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5001372.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11325..11693)
                     /gene="folB"
                     /locus_tag="ACHJ7J_003180"
     CDS             complement(11325..11693)
                     /gene="folB"
                     /locus_tag="ACHJ7J_003180"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO5001373.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11798..12415
                     /gene="plsY"
                     /locus_tag="ACHJ7J_003181"
     CDS             11798..12415
                     /gene="plsY"
                     /locus_tag="ACHJ7J_003181"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO5001374.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12428..13360)
                     /gene="ttdR"
                     /locus_tag="ACHJ7J_003182"
                     /gene_synonym="dan"
     CDS             complement(12428..13360)
                     /gene="ttdR"
                     /locus_tag="ACHJ7J_003182"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO5001375.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVRDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13567..14478
                     /gene="ttdA"
                     /locus_tag="ACHJ7J_003183"
     CDS             13567..14478
                     /gene="ttdA"
                     /locus_tag="ACHJ7J_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO5001376.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14475..15080
                     /gene="ttdB"
                     /locus_tag="ACHJ7J_003184"
     CDS             14475..15080
                     /gene="ttdB"
                     /locus_tag="ACHJ7J_003184"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO5001377.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRIKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15129..16592
                     /gene="ttdT"
                     /locus_tag="ACHJ7J_003185"
     CDS             15129..16592
                     /gene="ttdT"
                     /locus_tag="ACHJ7J_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO5001378.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLIPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16635..17648)
                     /gene="tsaD"
                     /locus_tag="ACHJ7J_003186"
     CDS             complement(16635..17648)
                     /gene="tsaD"
                     /locus_tag="ACHJ7J_003186"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO5001379.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17886..18101
                     /gene="rpsU"
                     /locus_tag="ACHJ7J_003187"
     CDS             17886..18101
                     /gene="rpsU"
                     /locus_tag="ACHJ7J_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO5001380.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18212..19957
                     /gene="dnaG"
                     /locus_tag="ACHJ7J_003188"
     CDS             18212..19957
                     /gene="dnaG"
                     /locus_tag="ACHJ7J_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5001381.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNTEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEERMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20152..21993
                     /gene="rpoD"
                     /locus_tag="ACHJ7J_003189"
     CDS             20152..21993
                     /gene="rpoD"
                     /locus_tag="ACHJ7J_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO5001382.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22072..22578)
                     /gene="mug"
                     /locus_tag="ACHJ7J_003190"
     CDS             complement(22072..22578)
                     /gene="mug"
                     /locus_tag="ACHJ7J_003190"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO5001383.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22703..22778
                     /locus_tag="ACHJ7J_003191"
     tRNA            22703..22778
                     /locus_tag="ACHJ7J_003191"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22736..22738,aa:Ile,seq:cat)
     gene            complement(22832..23596)
                     /gene="nfeF"
                     /locus_tag="ACHJ7J_003192"
                     /gene_synonym="yqjH"
     CDS             complement(22832..23596)
                     /gene="nfeF"
                     /locus_tag="ACHJ7J_003192"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO5001384.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         5698 a         6520 c         6084 g         5352 t
ORIGIN      
        1 gatgtttacc gcgattattg ccgtatgcat tctgtttatt attggaatta ttttcgccag
       61 gctctatcgt cgcgcttcgg cagagcaagc ttttgttcgt actggtttag gtgggcaaaa
      121 agtggtaatg agcggtggcg caatcgtgat gccgatcttt catgaaataa tccccatcaa
      181 tatgaatact ctgaagctgg aagtcagccg ctcaaccatt gatagcctga ttacgaaaga
      241 tcgtatgcgc gtcgatgtag tagtcgcttt ctttgtgcgg gtaaaacctt cagtagaagg
      301 gattgccacc gctgcccaga cgctggggca acgcaccctg tcgcctgaag acttacgtat
      361 gttggttgaa gataaatttg tcgatgccct ccgtgcaaca gctgcgcaaa tgaccatgca
      421 tgagttacag gatacccgcg agaacttcgt gcagggagta caaaataccg tagctgaaga
      481 tctgtcgaaa aacggcctgg aactggaaag cgtttcactt accaacttta accagacctc
      541 gaaagaacat ttcaatccga acaatgcctt tgacgccgaa ggtttaacca aactgactca
      601 ggagacggag cgccgtcgcc gcgagcgtaa cgaagttgaa caggatgtag aagttgcggt
      661 gcgtgagaaa aaccgtgatg cactttcgcg caagttggag attgaacaac aagaagcgtt
      721 tatgacgctt gagcaggagc agcaggttaa aacccgtacc gctgagcaga atgcgaaaat
      781 tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acgcgaattc tggctgaacg
      841 acagattcag gaaacagaaa tcgaccgcga acaggccgtc cgctcaagaa aagttgaagc
      901 tgaacgtgaa gttcgcatta aagagatcga acagcagcag gtcaccgaaa tcgctaacca
      961 gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag aagcgcgtgc
     1021 taaccttgca ctcgcagaag cagtaagcgc ccaacaaaac gttgaaacca ctcgccagac
     1081 cgcagaagcc gatcgtgcta aacaagttgc cctaatcgct gccgcgcagg atgcagaaac
     1141 caaagcggtt gaactgaccg tgcgggcaaa agcagaaaaa gaagccgcag agatgcaggc
     1201 ggcggcgatc gttgagttag ctgaagctac gcgtaaaaaa ggtctggcgg aagcagaagc
     1261 acaacgtgcg ctgaacgatg ctatcaacgt actttctgat gaacaaacca gccttaaatt
     1321 caaactggca ttattgcagg cgctgcctgc ggtaattgag aaatccgttg agccgatgaa
     1381 gtcgatcgat ggtattaaga tcattcaggt cgatggactg aatcgtggtg gtactgcggg
     1441 tgatgcgagc gcaggtagcg ttagtggagg aaaccttgca gagcaggcat tgtctgccgc
     1501 cctttcttac cgcacacagg caccgctgat tgactccttg ctcaatgaaa ttggcgtttc
     1561 aggcggctcg ctggcggcat tgacttcacc cttaacctca acaactcccg tcgccgaaaa
     1621 cgtagaataa attttcagct atgaacatcc ctggcacaaa acgccgggga ttttttatat
     1681 ctgcgttccg ctaaaaattg caaatgatca ggttgttgca acgttttgcg cgaccgctcg
     1741 gggaaggcaa aactgccaca gggaaagcat tgcgcgggat ccggcactca tcaacaatcg
     1801 ggaagtcagc aaggggctga aacgggaaag cccctcccga agaaggggcc ttgtataagg
     1861 aaagggttat gatgaagctc gtcatcatac tgattgtgtt gttactcgta agtttcgcag
     1921 cttattaaca gccaatcaga gggggagaaa tcctccctca cccttgttcc tttactttag
     1981 gttgaaaaaa aatcagcgtc aagaaggttt cattaacaca tatccctggc ccaaaaagcc
     2041 ggggattttt tatatctgcg ttccgctaaa aggcgcaaat gctcaggacg ttgcagcgtt
     2101 ttgcgtgacc gctcggggaa ggcaaaactg cttcagggaa agcgttgcgc ggggtccggc
     2161 gctcatcaac aatcggggag tcagcaaggg gctgaaacgg gaaagcccct cccgaggaag
     2221 gggccttata gaaggaaagg gttatgatga agctcgtcat catactggtt gtgttgttac
     2281 tgttaagttt cccgacttac taacaactca tcagaggggg gagaaatcct cccttaccct
     2341 tgttccttta ctctaggttg aaaaaacaac agcgtcaata ggcctgccat gtacgaagcg
     2401 agatctgtga accgctttcc ggttagcctt ttttatcctg ttggatcttc ttgataatgt
     2461 tagtggtcga gcaaccgtct tcaaagttga gcaccaacac ttcgccgccg ttggcccaga
     2521 cttctttact cccggcgatc tcttccggtt tgtagtcacc gcctttcacc agcaggtctg
     2581 gcaggatccc ggcgatcaga cgctgcggcg tgtcttcttc aaacgacact acccagtcga
     2641 ccgcttccag tgcgcccagc acaatcatac gctgttcgag tgggtttacc gggcgggaat
     2701 cccctttcag ccgtttggtg gaggcatcgc tgttgacggc aacaatcaag cggtcaccca
     2761 gcttgcgggc atttgccaga taagagacgt gcccggcatg taggatatcg aagacaccgt
     2821 tggtcattac cactttttca ccacgtttac gcgctgccgc tacagccagc ttcagttcct
     2881 cttcggtcat cacgccaaag ccggtatctg cgcgtccacg tacagcgttt tccagctcga
     2941 tcggcgaaac cgtggacgtc cccagtttgc cgaccaccac accagccgcc gcattggcaa
     3001 agaagcaggc ttcttccagc gaattacccg ctgccagcgt tgccgccagg acgccaatca
     3061 ccgtgtcgcc cgcaccggta acgtcataca cttcctgcgc ctgagttggc atatgcagcg
     3121 gcgctttacc cggttgcagc agcgacatac cctgttcgga acgggtcact aacagagccg
     3181 agagttcgta atcggcaatc agtttcatgc cgcgctcaac aatctcttct tcggtcttac
     3241 atttaccgac aacagcttca aattccgaga gattcggcgt taacagcgta gcgccgcggt
     3301 agcgctcaaa atcggtacct tttggatcaa tcagcaccgg aacacccgct ttacgcgcca
     3361 gttggatcat ctgctgtacg cttgccagcg cacctttggc gtagtcagaa agcaccagcg
     3421 cgccaatcga actcagcgcc tgattaatcc gctcgtgcag cggctgcgga tcaacacctt
     3481 cgaaaccttc ttcaaaatcc agacggatca gctgttggtt gcgggaaagt acccgtaatt
     3541 tggtgatggt cggatgcgtc ggtacagaaa cgaagtcgca tttgacgttg acgtcggcca
     3601 gagatttact cagcgcgcgc gctgcatcgt caatgcccgt caacccgacc agacgtgcat
     3661 tagcaccgag agaagcgata ttcatcgcca cgttagccgc gccgcccggg cgttcttcga
     3721 tggtattcac tttaaccacg ggcaccggcg cttccggcga gatacggctg gtgggaccgt
     3781 accagtaacg atccagcatc acatcaccaa ccaccatcac tcctgctcgt tcaaactctg
     3841 gcagcgttac tttcattcct gtctcctgag agattcaaaa tttgcgcgcg ataataccat
     3901 acttcattct tccaccagcc acttctgcca gcttgcccgc accaaatcac gctctgcggt
     3961 gaagcaatcc tccgacacat ggcccggcaa ttcctgtaat gccagatgat gaagttcatc
     4021 gcgaagcgtc gtgtaagcac gggtcagcgc catcgcttcc tgctcttcca taatgtcgtt
     4081 ttgcgccagc agttccagaa tgcgcacgtt gtctgaccag cgcgttaact tcggtttttc
     4141 atgagcgtag cgcaacacca gatattgggt aataaattcg atatcggtaa ttccgccttc
     4201 atcggctttg atatcaaagc gatcgcgatg tttattgccg agatgagcgc gcattttctc
     4261 gcgcatttcc cgcacttctg tttgcagggt tttgccttcg cgcggcagcg ccataatctc
     4321 acgacgcacc gcgtcaaagt gcgcggtgag ctgcggatcg ccgtacacta cacgcgcacg
     4381 caccagcgcc tgatgttccc acgtccaggc ctcgtttttc tgataatcgg caaatgcttc
     4441 tgcggatgtc accagcattc ccgccgcccc ggacggacgc agtcgagcat ccacttcata
     4501 caaaatgccg gaagaggtac gcgtactgaa cagatgcata atgcgttgcg ccagacgcag
     4561 ataaaactgc cgcccgtcga tttcccgctc accgtcagtc atcgcatcca ttgggcaatc
     4621 atggaggaag ataaggtcaa gatcggaact gtagcctaac tcccagccgc ccagcttgcc
     4681 gtagccgacc accgcaaaac cacgcccttc gcgatcgttc aggtgatttg gcttaccgta
     4741 gcgggcaacc atttgaaccc acgcctgctg aacaacggcg tcaatcatgg cttccgccag
     4801 ccaggttaag tgatcgctca ctttcatcac cggtagcgta ccggcgatat ccgctgcggc
     4861 gatgcgtaac agctgcgcct gtttgaactg acgcagcgcc tcaagctgtt gctcttcgtc
     4921 atcttccggc acgcgcagca aatactggcg caactcatcg cggtaggcat cggtcgccgt
     4981 cggctggtaa agggtgtttg gatcgagcaa ttcatccagc aataatggat aacgcgccag
     5041 ctggctggca atcatcggcg acgctgcaca cagagaaatc aaatgtttaa gcgccgcggg
     5101 gaattcactg agcaattcta aataggtggt gcgggtaaca atccccacca gcaaggcggt
     5161 aatgcgcgac agcgtgacgg cagcgtcttc acgcgcacag acatcactta gcagatgtgg
     5221 catcagatgg tcgagcacct gacgtcctcg cggcccgatg gtgcgcttat ccagctcttt
     5281 gcggaaatca gcaatcagcg ttagcacctg tttgcgatca ttttcgctaa gatgcgccag
     5341 cactggcgtg gtgtcatctt cctgcaacgc atcctgccac agctcacgcc actgttccga
     5401 cagcgactct tcctgagttt cactttcatc gtcgccaatc aattcattaa acacccggcg
     5461 cacattggtc atatgtgcgg tcagcgccac ggtcagttgc ggccagtcag caaagtccat
     5521 cgcccacgcc agccgcgcac ggttaagctc atcagaagga agcgtctggg tttgttcgtc
     5581 gttaatgctt tgcagcaggt tttccagacg ccgcaggaac agatacgcca ctcgcaattg
     5641 ttcagcatcg ttttcagaaa gcagatgcag cgcggcaatg acgctgagcg ttggcagtaa
     5701 agagcgcgac tgcagcgacg gttcgcgccc accgcgaatg agctggaaca cctgaacgat
     5761 aaattcaatt tcgcgaatgc cgcctgcgcc gagtttgata ttgtcggtca aaccacgtcg
     5821 acgcacttca cgggcaatca tccctttcat gttgcgcagc gactgaatca cgctgaaatc
     5881 gatgtaacga cggaaaacaa acgggcgcag catcgcacgc aactcgttag catagacgcc
     5941 ttcgctatcg cccataatcc gcgccttgac catcgcgtaa cgctcccagt cgcgcccctg
     6001 ctcctggtaa taatcttcca gcgccgcaaa gctcagcacc agcgggccac tttcgccaaa
     6061 cggacgcagc cgcatatcca cgcgatagac gaagccatcc tgcgttggtt gatccagcac
     6121 tttaatcagc cgctgcccca tgcgggtaaa aaactgggcg ttatccagtt cccggcgtcc
     6181 accctgcgta caaccatgtt ccggccaggc aaaaatcaga tcgatatcag aggagaaatt
     6241 cagttccccg ccgcccagct tacccatgcc taaaatcagc agcggttgcg cttcgccctg
     6301 cgcattgcac ggcgttcccc actcgcggca gcaggcgtca tatagccagt cacgcgccgc
     6361 aacaatcagc gtctccgcca gatagctgag ctgctgcaat atgctctctt cagtaaccag
     6421 tgccagcgtt tgcgcccagg cgatacgtac catgatgcgc cgacggaaca gccgcagctc
     6481 gcgcattaac ccggcttcgt cactcacatt actgagcgcc tcctgcaacc atgcaacgta
     6541 atgctgccat tcgtcggcct gcggcggttg gctttccagt tccgtcagcc actctggatg
     6601 cgcaatcacg ctgtcctgca caaaatcact gaaagtaagt actgacttcg cctgtgcgct
     6661 aagtgattcc tcggctaaag gctctggcag ccgctcaaca acggtctgcc agtactgctg
     6721 taacggtgaa gagagcggct tcataagcga ttttatcctt gcctgattaa cgttttccgc
     6781 tgtgcaacca gaacggttcc tgattgtttg cctcattacg gaaatgttca atttcgatgc
     6841 gttgcccggt cgcaatagcg tgatgcagcc cctgccagtt ctccagccag gcttgcgcga
     6901 cgacaggatc atagtaaccc gccagcaaca gtattgagtc aatatcacgc gtcaggcgtg
     6961 gcaactggtc acggtagcga tcgcctaacg gctggcagaa aacgcttttc agttcagcgg
     7021 catggcggga aagatggata tcggcaaagc gtttgaagga gtcgctgatt ttgccctgcg
     7081 ctttggcatc taaaaatggc tgccatgctt tgcttaccaa ccattcggtc aacgccagct
     7141 tcgccattgc cgtttcggta gagtagaccg ccgtcacggc agaaaccgca gaagcaatgg
     7201 tcgcctcgca ttgagtcagc agatcacgta agtgagtgct cgctttacgc ggcacaatac
     7261 caccgaacag catcagcgta tgacggacca ggctaatggc tgccagcacc tgttctttcg
     7321 ccgcatcgtt gccgcgtacc cacagttctt catgatactg ccattgcgct aacgccagct
     7381 cgagcgccgc ttccagcccc tgctcgacat ccgcttttgc cgcaacatgt aaaatggtgg
     7441 tcggtttgat ttcacgcgcc ggattgccct gtgccagatg atagccacgc gccgctttgc
     7501 tcaggctgcc ctggcgtaat ccggtttgcg ataccagttg gttcgccagt ttcagcaccg
     7561 cacgcatgtc gccgctaagc agctccagtt ccagctcaca gataggttca gcaaattcac
     7621 ccgctttcac ctccccctga tcgagagcga tttcaattcg gcttccatcg accgccacca
     7681 gccatttttc gcgataaaaa tcggtgctga acagcggttg tacgcgggaa gcgagatcgg
     7741 caggcaattc gccgttcggc cagacttccg agggaaactg cgccaggtcg agcgtcggtt
     7801 cgctcaacgc cacgttatat tccgggcgct ggtgtaagcc gcctgtcact cttcctgcga
     7861 ctttcatggt catctcatag cgaccgtttt cgccacgaat acgcaggccc atatcgtgcc
     7921 cacgcagcca gttatccggc gtttcgtagt aaatattcag caactgcacg gggtcatggt
     7981 gctcgccgcc cagcgtattg agatggtcac gcaacgcctc aacggcactg tgattaacaa
     8041 taaactttaa ttcgatttcc tgagccatgg cctttactta tcggttatgt cacataagtg
     8101 acgatgaacg gcgaacttaa tgcaatcttt tttgtcagta gatagtattt tgcgccaaat
     8161 tgccatgcaa cgggcaattt gacgggcgta aaagtttgaa gcagtggcaa gacctgacac
     8221 agaggattcc gattgatgtc gaatgctttg cgtagcgaca ctgatgccac tactatcgtt
     8281 ccactttcca tgacaataac gacagcctga tgccaaaatt acgcctgatc ggattaactt
     8341 tactcgcact tagcgcgact gccgtctcac acgctgaaga aacgcgctat gtttccgacg
     8401 aactgaatac ctgggtccgc agcggtccgg gagatcatta tcgcctcgtg ggcacggtta
     8461 acgccggcga ggaagtgacc ttattacaaa ctgacgccaa caccaattat gcccaggtga
     8521 aagacagctc tggccgtacc gcctggatcc cgttgaaaca acttagcact gagccaagcc
     8581 tgcgctcccg tgtgccagat ctggaaaatc aggtcaaaac cctgaccgat aaactcacca
     8641 atatcgataa cacctggaat cagcgcaccg cagaaatgca gcaaaaagtg gcgcagagcg
     8701 acagcgtgat caacgggtta aaagaagaaa atcagaaact gaaaaacgag ctgattgtcg
     8761 cgcagaaaaa ggtcgatgcc gccagcgtac agctggatga caaacagcgc accatcatca
     8821 tgcaatggtt tatgtatggc ggtggcgtgc tggggcttgg cttgctgctc ggtctggtac
     8881 tgccgcacct gatcccaagc cgcaaacgca aagatcgctg gatgaactaa atcgccttct
     8941 tcgttgcact gtcttattat taggaattgt gtatttgacg aagggatgat ggcgtgaaga
     9001 tttatctggt cggtggtgct gttcgggatg cattgttagg gctaccggtc aaagacagag
     9061 attgggtagt ggtcggcagt acgccacagg agatgctcga cgcgggctac cagcaggtag
     9121 gccgcgattt tcctgtgttt ctgcatccgc aaacgcatga agagtatgcg ctggcacgta
     9181 ccgaacggaa atccggctcc ggttacaccg gttttacctg ctatgccgca ccggatgtca
     9241 cgctggaaga tgatcttaag cgtcgcgatc tgaccattaa tgcgctggcc caggacgata
     9301 acggcgagat tatcgacccg tacaacggtc tgggcgatct gcaaaatcgt ctgttgcgcc
     9361 atgtttcccc cgcttttggc gaagatccgt tacgcgtatt gcgcgtggcg cgttttgctg
     9421 cgcgttatgc ccacctcggt tttcgtattg ccgatgaaac tctgacgctg atgcgcgaga
     9481 tgacccatgc gggtgaactg gaacacctga cgcctgaacg ggtatggaaa gagacagaaa
     9541 acgccctcac cacccgcaat ccacaggtgt tcttccaggt actgcgcgat tgcggcgcac
     9601 tgcgcgtttt attcccggaa attgacgccc tgtttggtgt tccggcccct gccaggtggc
     9661 atccggaaat cgacacgggt attcatacct taatgacgct ctcaatggcg gcgatgctga
     9721 gtccgcaggt cgatgtccgt ttcgcaactt tatgccacga tctcggtaaa ggactgacgc
     9781 cgccagaact ctggccgcgt catcacggtc acggcccggc gggagtgaag ttagtggaac
     9841 aactatgcca gcgtctgcgc gtgcctaatg aaattcgcga tttagccaga ctggtggctg
     9901 agtttcacga tctcatccac accttcccaa tgctgaaccc aaaaaccatc gtcaaattat
     9961 ttgattccat cgatgcctgg cgtaaaccgc agcgtgtcga gcaactggcg ctgaccagcg
    10021 aggctgacgt gcgcggcaga accggttttg aatcagcgga ctacccgcaa ggccgttggt
    10081 tgcgcgaagc ctgggaagtg gcgcagtcag tgccgacaaa agccgtcgtt gaagcgggat
    10141 ttaaaggcgt ggagattcgc gaggagctga cccgacggcg gattgcggcg gtagccagct
    10201 ggaaggaaca acgttgccca aagcctgaat gagtattggg cattttgccg gatgcggcgt
    10261 aaacgcctta tccggctaca cgtcaacgac agttgtaggc ctgataagac gcgcaagcgt
    10321 cgcatcaggc gttgattgcc ggatgcggcg tgaacgcctt attcggccta cgcaatcgtg
    10381 gacaacgcca ggcatccgac actattcctc aattaaaaga acacgacata caccgcagcc
    10441 gccacaataa agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg
    10501 aaggttttaa tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc
    10561 gggatatcgc cgcttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc
    10621 atcatcatcg gcaccgccag caggaacgaa aactcggaag cagcgtaacg gctcaccccc
    10681 atcagcatcc cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga
    10741 cactggaaac agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca
    10801 cgcggctctt tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc
    10861 gcatacatca catttatcgg gttaaacaat gacttaatcg tgtcgtggaa caacagcccc
    10921 agtaccaccg ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt
    10981 tctccttcgt gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc
    11041 cagaacatca ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc
    11101 gccgtgtcgc cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat
    11161 accggcagaa attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag
    11221 tgcatatcgc tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact
    11281 gcagtatgac caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc
    11341 agattattgc cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc
    11401 ttgctgagtt tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc
    11461 tcttccgcca cgcgctccac cagcgcaaaa cgtgccccct cgacgtggct gaccaccgtt
    11521 tctgcaatgt cagcgtagct gaggcaatcc gccacatcat cacttttcgc cgctttacgg
    11581 ttatcccacg ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg
    11641 taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc
    11701 tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat
    11761 tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat
    11821 gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg
    11881 tgggctgccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg
    11941 tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt
    12001 gcctgtctgg ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat
    12061 cgccgcctgt cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt
    12121 tgctaccgct tttggagcca tcgcaccaat tggctgggat ctcaccggag taatggcggg
    12181 aacctggtta ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc
    12241 actgattgct ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat
    12301 gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca
    12361 ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct
    12421 ggtggatcta catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg
    12481 ccgccagaaa ttccacgcag acacgtaatt tcatgctgcg atagagcggc tcccggtaaa
    12541 cagcccagat attggcgctt tgtgcatact ccggcaatac ttgcaccagt ttgccactct
    12601 ccagaaacgg caacacatcc cactcggaac gcagcataat ccctttgccc tccagcgccc
    12661 attgcagcac aatctcgccg ctattggagg aaagatgccc gctaaccttc accgattttt
    12721 tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct ttggtcacca
    12781 gacagtcatg acgacttaat tcctgtaggg attgtggctg cggatatttt tgcagatact
    12841 caggtgctgc gcataatatt cttttatttt tcgttaacag atgcgcaata taataatcag
    12901 gaatttcgtc attaatacga atatccagat caatattatc ccgcactaaa tcaatttgtc
    12961 gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcatcagt tcggtaatgg
    13021 ctggcgcaat atggctgcgc ccaaaaccaa aactgcaacc aatacgaatc atcccctccg
    13081 ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg atattgggtg aggatttcca
    13141 gcgcatgttc gtaacaacgt tgaccacttt cagtcagcgc cacgccgcga gcggagcggt
    13201 tcagcagcgt tgttgccaac gtattttcaa ggatttggat ccgcttggtg acaaatgcgg
    13261 gcgtctgccc aagcgtcgcc gctgcggcgc taaaactccc gctgtggacg atttctacca
    13321 gtacctgcaa gtctttggct aaaggccagc tattcagcat cgttaattat ccgcagttgt
    13381 gataagcgca gtgtatttcg caaaacattg ccattccctg ctctggcgca atattcattc
    13441 ttattttcgc gttgattcac gaactgttaa ttacatattc acggtggcaa aaaatataaa
    13501 accacatttt tagtggtagt ttgtggcggt gaatttttcc agacaaatac aaaaactgga
    13561 gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac ggagattgtc
    13621 gctaacttta ccgccatgat ttctacccga atgcctgatg acgtggtgga taaactaaaa
    13681 cagctaaagg atgccgaaac gtcgtcgatg gggaaaatca tctaccatac gatgttcgac
    13741 aacatgcaaa aagcgattga cctgaatcgt cctgcctgtc aggacaccgg ggagattatg
    13801 ttcttcgtta aagtcggttc ccgcttccca ctgcttggcg agctgcaaag catactcaaa
    13861 caagccgtgg aagaggcaac cgtcaaagcg ccactacgtc acaatgcggt agaaattttt
    13921 gacgaagtga acacaggcaa aaataccggc agcggcgtac cgtgggtcac ctgggatatc
    13981 gtccccgacg gtgacgatgc ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta
    14041 ccaggccgct cgaaagtgtt aatgccgtca gaaggctacg aaggcgtggt gaaattcgtc
    14101 ttcgaaaata tctccaccct cgccgtaaac gcctgtccac cggtactggt gggcgtgggc
    14161 atcgccacct cggtggaaac cgccgccgta ctctcgcgta aagccatttt gcgcccgatt
    14221 ggcagccgcc accccaatcc aaaagcggca gagctggagc tacgcctgga agaaggactc
    14281 aaccgtctgg ggattggtcc acaagggctg actggcaaca gttcagtgat gggcgtgcat
    14341 atcgaatctg ccgcccgcca tccgtcaacc atcggcgttg ctgtttctac cggctgctgg
    14401 gcgcatcgtc gcggcacact gctggttcat gccgatctca cctttgaaaa tctgtctcac
    14461 acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa gatctgcaag
    14521 atattcacgt cggcgatgtg atctatctga ccggtacgct ggtgacctgc cgtgacgttt
    14581 gtcaccgccg attgatcgaa ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga
    14641 ttttccacgc tggccccatc gtgcgcaaaa acggcgacaa atgggagatg gtttccgttg
    14701 gtccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgag cagaccggcg
    14761 tgaaactggt ggtcggcaaa ggcggaatgg ggccgctgac cgaagaaggc tgccagaaat
    14821 tcaaggcgct acatgtgatt ttcccggcag gctgcgcggt gctggcggca acccaggtgg
    14881 aagagattga agaggtgcac tggacagagc tcgggatgcc ggagtcactg tgggtctgcc
    14941 ggatcaaaga gttcggcccg ctgattgtct ctattgatac ccacggcaac aacctgatag
    15001 cagaaaacaa aaagctgttc gccgaacgcc gcgatcccat cgtggaagag atctgcgagc
    15061 acgtccatta catcaaataa ccctcccgga gaggctcgcc ccctctcctt tttcgcaggc
    15121 ataacacgat gaaaccttcc actgaatggt ggcgatacct tgcgccgctg gcggtcatcg
    15181 ccattattgc tctaattccg gttcccgcag ggctggagag tcatacctgg ctctactttg
    15241 ctgtttttac tggcgtaatc gttggactga tcctcgaacc cgtgccgggt gccgtggtgg
    15301 cgatggtggg tatatccatc atcgccatac tctctccctg gctgctgttc agcccggagc
    15361 agctcgctca gccaggcttt aaattcactg caaaatccct ctcgtgggcc gtttccggtt
    15421 tttctaattc ggttatctgg ctgattttcg ccgcctttat gtttggcaca ggctatgaaa
    15481 aaaccgggct ggggcgacgt atcgcgctga ttctggtgaa aaagatggga catcgcacgc
    15541 tgtttctcgg ctatgcggtg atgttctccg agctgatcct cgcacctgtc acaccgtcca
    15601 actcggcgcg tggtgcgggg attatctatc ccatcatccg taacctgccg ccgctctatc
    15661 aatcacaacc aaacgacagc agttcgcgca gtattggctc gtacatcatg tggatgggta
    15721 ttgttgccga ctgcgtgacc agcgccattt tcctgacggc gatggctcct aacttgctgt
    15781 taattggact gatgaaaagc gcatctcacg ccacgctgag ttggggcgac tggttcctcg
    15841 ggatgttgcc gctcagtatt ttactggtcc tgctgattcc ctggctggct tacgtgttgt
    15901 acccgcctgt actgaagtca ggcgatcagg tgccgcgctg ggcagagacg gaactgcagg
    15961 caatgggccc gctctgttcg cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg
    16021 tgctgtggat tttcggcggt gattatatcg atgccgcgat ggtcggttac agcgtggtgg
    16081 cgctgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat aaagcggcgt
    16141 ggaacgtttt cttctggctg gcctcgctta tcaccctcgc gaccggactc aacaacaccg
    16201 gttttattag ctggtttggc aaactgttag caggcagctt aagcggttat tcgccaacga
    16261 tggtgatggt ggcgttgatt gtggtgtttt atctactgcg ctactttttc gccagcgcca
    16321 cggcgtatac ctccgccctc gcaccgatga tgattgccgc cgcgctggcg atgccggaaa
    16381 tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat tggtctgggg agcattctta
    16441 caccatacgc caccggaccc agtccgattt actacggtag tggttatctg ccaacggcgg
    16501 attactggcg actgggggcg atttttgggt tgatattcct cgtgttgctg gtgattaccg
    16561 gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa attgctctcc
    16621 ggcacccaat agttttacgc agccggtaac tccgccagcg gccagcgcgg acgtacgcta
    16681 acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata ggcgatcatc
    16741 gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc
    16801 atcatttcag ccagcttcgc ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc
    16861 agtcgcttaa agcccgtctg atccagcgca cgcttacact taatcatcaa cgtatcgacc
    16921 accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca
    16981 cgaatcgtgt tcgccgcaaa ggttttcaga ccagaaaagc tgaaatccag ccccggacgg
    17041 tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac cctgagccgc cattttcgac
    17101 agtaacggtc cgccaggata atccagcccc agcagcttcg cggttttatc aaacgcttca
    17161 ccggcggcat catcgataga ctcgccgagc agctcgtact gaccaatgcc agtcacgctg
    17221 attaactgcg tatgaccgcc ggaaaccagc agcgcaacaa acggaaattc cggcgggtta
    17281 tcttccagca tcggcgctaa cagatgccct tccatatggt gtacagggat cgccggaacg
    17341 tcccaggcaa acgccagaga acgccccacg gtcgcgccaa ccagcagcgc gccgactaat
    17401 ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaaacc agactccttt
    17461 agcgccgcct ggatcaacgg tacggtttta cgcacatgat cgcgggaggc cagctcaggc
    17521 acaacgcccc cgtagtcagc gtgcaatttc acctgactat acaattggtt ggctaacaaa
    17581 cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc aatacccagt
    17641 acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca atgcgggttg
    17701 atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc cttataaaag
    17761 tccctttcaa aaaaggccgc ggtgctttac aaagcagcag caattgcagt aaaattccgc
    17821 accattttga aataagctgg cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga
    17881 ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct
    17941 tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg
    18001 aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga
    18061 aactggctcg cgaaaacgca cgccgcactc gtctgtacta attccccgag agcgttgctc
    18121 tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt
    18181 ttcgtttatg aattgctaaa aatcggggcc tatggctgga cgaatcccac gcgtattcat
    18241 taatgatctg ctggcacgca ccgacatcgt cgatctgatc gatgcccgtg tgaagctgaa
    18301 aaagcagggc aagaatttcc acgcgtgttg tccattccac aacgagaaaa ccccgtcctt
    18361 caccgttaac ggtgagaaac agttttacca ctgctttgga tgtggcgcgc acggcaacgc
    18421 gatcgacttc ctgatgaact acgacaagct cgagttcgtc gaaacggtcg aagagctggc
    18481 ggcaatgcac aatcttgaag tgccatttga agcaggcagc ggccccagcc agatcgagcg
    18541 ccatcagcgg caaacgcttt atcagttgat ggacggtctg aatacgtttt accaacaatc
    18601 tttacagcaa ccagttgcca cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca
    18661 cgaggttatc gcccgctttg cgattggttt tgcgcccccc ggctgggaca acgtcctgaa
    18721 gcggtttggc ggcaatccag aaaatcgcca gtcattgatt gatgcgggga tgttggtcac
    18781 taacgatcag ggacgcagtt acgatcgttt ccgcgagcgg gtgatgttcc ccattcgcga
    18841 taaacgcggt cgggtgattg gttttggcgg gcgtgtgctg ggcaacgata cccccaaata
    18901 cctgaactcg ccggaaacag acattttcca taaaggccgc cagctttacg gtctttatga
    18961 agcgcagcag gataacactg aacccaatcg tctgcttgtg gtcgaaggct atatggacgt
    19021 ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg tcgttaggta cgtcaaccac
    19081 cgccgatcac atacaactgt tgttccgcgc gaccaacaat gtcatttgct gttatgacgg
    19141 cgaccgtgca ggccgtgatg ccgcatggcg agcgctggaa acggcgctgc cttacatgac
    19201 agacggccgt cagctacgct ttatgttttt gcctgatggc gaagaccctg acacgctggt
    19261 acgaaaagaa ggtaaagaag cgtttgaaga gcggatggag caggcgatgc cgctttccgc
    19321 atttctgttt aacagcctga tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg
    19381 tttgagtacg ctggcactgc cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata
    19441 tcttcgtcag gaattaggca acaaattagg catacttgat gacagccagc ttgaacgatt
    19501 aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac
    19561 catgcgtata cttatagggt tgctggtgca aaatccagaa ttagcgacgc tggtcccgcc
    19621 gcttgagaat ctggatgaaa ataagctccc tggacttggc ttattcagag aactggtcaa
    19681 cacttgtctc tcccagccag gtctgaccac cgggcaactt ttagagcact atcgtggtac
    19741 aaataatgct gccacccttg aaaaactgtc gatgtgggac gatatagcag ataagaatat
    19801 tgctgagcaa accttcaccg actcactcaa ccatatgttt gattcgctgc ttgaactgcg
    19861 ccaggaagag ttaatcgctc gtgagcgcac gcatggttta agcaacgaag aacgcctgga
    19921 actctggaca ttaaaccagg agctggcgaa aaagtgattt aacggcttaa gtgccgaaga
    19981 gcgatcggga agcccccgac agccgcactg agaggcagcg gcaaatatat aagtacgccc
    20041 tcgtaattat cgttggcggt aaacaaccgt tggatttcag cgttaacggc tgaaggacat
    20101 cgggtcaatc gcccaacacc aacctcatga aataagtgtg gataccgtct tatggagcaa
    20161 aacccgcagt cacagctgaa acttcttgtc acccgtggta aggagcaagg ctatctgacc
    20221 tatgccgagg tcaatgacca tctgccggaa gatatcgtcg attcagatca gatcgaagac
    20281 atcatccaaa tgatcaacga catgggcatt caggtgatgg aagaagcacc ggatgccgat
    20341 gatctgatgc tggctgaaaa caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg
    20401 ctttccagcg tggaatctga aatcgggcgc acgactgacc cggtacgcat gtacatgcgt
    20461 gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt
    20521 gaagacggga tcaaccaggt tcaatgctcc gttgctgaat atccggaagc gatcacctat
    20581 ctgctggaac agtacgatcg tgttgaagca gaagaagcgc gtctgtccga tctgatcacc
    20641 ggctttgttg acccgaacgc agaagaagat ctggcaccta ccgccactca cgtcggttct
    20701 gagctttccc aggaagatct ggacgatgac gaagatgaag acgaagaaga tggcgatgac
    20761 gacagcgccg atgatgacaa cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa
    20821 ctgcgcgctc agtacgttgt aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct
    20881 gctgctcagg aagagatcct gaaactgtct gaagtattca aacagttccg cctggtgccg
    20941 aagcagtttg actacctggt caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa
    21001 gaacgtctga tcatgaagct ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt
    21061 accctgttta ccggcaacga aaccagcgat acctggttca acgcggcaat tgcgatgaac
    21121 aagccgtggt cggaaaaact gcacgatgtc tctgaagaag tgcatcgcgc cctgcagaaa
    21181 ctgcagcaga ttgaagaaga aaccggcctg accatcgagc aggttaaaga tatcaaccgt
    21241 cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac
    21301 ttacgtctgg ttatttctat cgccaagaaa tacaccaacc gtggcttgca gttccttgac
    21361 ctgattcagg aaggcaacat cggtctgatg aaagcggttg ataaattcga ataccgccgt
    21421 ggttacaagt tctccaccta cgcaacctgg tggatccgtc aggcgatcac ccgctctata
    21481 gcggatcagg cgcgcaccat ccgtattccg gtgcatatga ttgagactat caacaaactc
    21541 aaccgtattt ctcgccagat gctgcaagag atgggccgtg aaccgacgcc ggaagaactg
    21601 gctgaacgta tgctgatgcc ggaagacaag atccgcaaag tgctgaagat cgccaaagag
    21661 ccaatctcca tggaaacgcc gatcggtgat gatgaagatt cgcatctggg ggatttcatc
    21721 gaggatacca ccctcgagct gccgctggat tctgcgacca ccgaaagcct gcgtgcggca
    21781 acacacgacg tgctggctgg cctgaccgcg cgtgaagcga aagttctgcg tatgcgtttc
    21841 ggtatcgata tgaacaccga ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc
    21901 cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt
    21961 tctgaagtgc tgcgtagctt cctggacgat taatcggtag gccggatcag gcgttacgcc
    22021 gcacccggca cctgtccctt tgcacaaacg ccaccttttc ggtggcgttt tttatcgccc
    22081 acgcactacc agcgcctggt ccagctcgcg atacgcttca accagtttct ccagtgaaac
    22141 gcgacttaaa ccgctgggat ttggcagcac ccaaatctgc gtcgaaccaa tgctgagcgt
    22201 ttgtttcccc cactgtgcac cgcgctggct gaatccctgt tcatatgctt gtttacccag
    22261 aatcgccaac gcttgcggct gataatcttc aattttttca atcagcttac gcccacctgc
    22321 gtgtagctct tgctttgaaa tttcattggc ttgcaccgtt ggacggtcta ccagtttggt
    22381 gacgccacaa cgataatcca gcagatgctg tgcctcctgc ggcttcaact gacggtcggt
    22441 aaacccggcc tgatatatca ccttccagaa gcgatttgcc ggatgagcaa agggaaaacc
    22501 agtcccggcg gatgaaagcc cagggttgat accgcaaaac acgacccgta accctggagc
    22561 caaaatatcc tcaaccatat ttactccttt ctaacatcat caggaaaagt ataacggatt
    22621 gaaaatacat tgtttataaa aacagcaggc gcgcggtaat ggctggattg cgacacggag
    22681 ttactttata atccgctacc atggcccctt agctcagtgg ttagagcagg cgactcataa
    22741 tcgcttggtc gctggttcaa gtccagcagg ggccaccaga tatagcaaag gctgacgaga
    22801 aatcgtcagc ctttttcttt ttatatatca gttactttgc gtgccagtaa gccgctgcac
    22861 gtacccgctg tgggtcatac tgttccgctt caaagcggcg gcttaaattc ttaacgactt
    22921 taccttcgcc ggttatccag atgaagtaat catcggcagg gatttgcatc tgcgccagac
    22981 gcgcatctac cgcctgctca tcatgtgcca gccattcgat attaaaacca tcaaggtgcg
    23041 cgagataatc ctgacaggcg ttatcccgca cgctaaccag cgcactaact tgcggtttaa
    23101 cggcaagttt gctcaacgtt tccaggcggc ggcgcaatgc gggcattccg gattcatcgc
    23161 agacatacag ctgatacgcg taatcttccg gcaccaccag cgaaccgcgc ggacctgcca
    23221 ccgtgagttt atcgcccggt tgggcctgca ttgcccagct actggcgacg ccgtcttcgt
    23281 gaataaagaa atcaatcgcc agttcatggc gtagttcgtc atacagtggc gtatagtcac
    23341 gcgacggtgg gcgtggtcct tccggccaga cgatgccctc ttccgttacc gttggcggca
    23401 caaagtgagc gtcaggttga ggaaagaaga gtttgctgtg atcgtcaaag ccgcgcgatg
    23461 taaaaccgtc cagcgcctcg ccgccaagga caatgcgctg aaaaccggcg ctgatacgct
    23521 caacgcgtag cacagtcagt tcacggaagc gcagatcatt gcgaacgcgc tgcgggtagc
    23581 ggggggagtt attcattgtt atcgccttcg tgatggtaat cagatatatc taaataattt
    23641 tcgtaaatga taat
//
LOCUS       DAZFFG010000074        23367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFFG010000074 DAZFFG010000000
VERSION     DAZFFG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.074"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1082
                     /locus_tag="ACHJ7J_003193"
     CDS             <1..1082
                     /locus_tag="ACHJ7J_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001385.1"
                     /translation="GPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASRLIRRG
                     HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRDAALQT
                     AAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTGAGNIS
                     ALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSGVAGGAVGSVA
                     GGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWL
                     AGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPV
                     MEYDASHLGNMVNRLEEALPPELPA"
     gene            1158..1613
                     /locus_tag="ACHJ7J_003194"
     CDS             1158..1613
                     /locus_tag="ACHJ7J_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO5001386.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            1692..1925
                     /locus_tag="ACHJ7J_003195"
     CDS             1692..1925
                     /locus_tag="ACHJ7J_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5001387.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPRMHFRRVIR
                     SAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            2025..2843
                     /locus_tag="ACHJ7J_003196"
     CDS             2025..2843
                     /locus_tag="ACHJ7J_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5001388.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYSDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            2898..3383
                     /locus_tag="ACHJ7J_003197"
     CDS             2898..3383
                     /locus_tag="ACHJ7J_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5001389.1"
                     /translation="MKTISHNSTTSSVSVTTASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            3399..3875
                     /locus_tag="ACHJ7J_003198"
     CDS             3399..3875
                     /locus_tag="ACHJ7J_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5001390.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3938..4159
                     /locus_tag="ACHJ7J_003199"
     CDS             3938..4159
                     /locus_tag="ACHJ7J_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5001391.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            4322..4690
                     /gene="cbeA"
                     /locus_tag="ACHJ7J_003200"
     CDS             4322..4690
                     /gene="cbeA"
                     /locus_tag="ACHJ7J_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO5001392.1"
                     /translation="MSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            4780..5154
                     /locus_tag="ACHJ7J_003201"
     CDS             4780..5154
                     /locus_tag="ACHJ7J_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5001393.1"
                     /translation="MKTLPDTHVREASSSPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            5151..5639
                     /locus_tag="ACHJ7J_003202"
     CDS             5151..5639
                     /locus_tag="ACHJ7J_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5001394.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            5651..5848
                     /locus_tag="ACHJ7J_003203"
     CDS             5651..5848
                     /locus_tag="ACHJ7J_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5001395.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            5945..6514
                     /locus_tag="ACHJ7J_003204"
     CDS             5945..6514
                     /locus_tag="ACHJ7J_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5001396.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFEWGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPLPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     SAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            complement(6795..7331)
                     /gene="yghD"
                     /locus_tag="ACHJ7J_003205"
     CDS             complement(6795..7331)
                     /gene="yghD"
                     /locus_tag="ACHJ7J_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO5001397.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(7333..8511)
                     /gene="gspL"
                     /locus_tag="ACHJ7J_003206"
     CDS             complement(7333..8511)
                     /gene="gspL"
                     /locus_tag="ACHJ7J_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO5001398.1"
                     /translation="MSSMLEIFFPLCTTDPIRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWFSVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAALKIDQQQ
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSMPCGYMAGDAQRIICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRDQA
                     EKQFLTLFPEQKRIVNLRSQVTMALNKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVMLRRK
                     "
     gene            complement(8508..9485)
                     /gene="gspK"
                     /locus_tag="ACHJ7J_003207"
     CDS             complement(8508..9485)
                     /gene="gspK"
                     /locus_tag="ACHJ7J_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO5001399.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNVLAQPTTASRPLALQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(9482..10087)
                     /gene="gspJ"
                     /locus_tag="ACHJ7J_003208"
     CDS             complement(9482..10087)
                     /gene="gspJ"
                     /locus_tag="ACHJ7J_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO5001400.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMTQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQENWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(10084..10455)
                     /gene="gspI"
                     /locus_tag="ACHJ7J_003209"
     CDS             complement(10084..10455)
                     /gene="gspI"
                     /locus_tag="ACHJ7J_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO5001401.1"
                     /translation="MKRGFTLLEVMFALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPQNTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(10452..11015)
                     /gene="gspH"
                     /locus_tag="ACHJ7J_003210"
     CDS             complement(10452..11015)
                     /gene="gspH"
                     /locus_tag="ACHJ7J_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO5001402.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPHGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLSQCDERMP"
     gene            complement(11019..11474)
                     /gene="gspG"
                     /locus_tag="ACHJ7J_003211"
     CDS             complement(11019..11474)
                     /gene="gspG"
                     /locus_tag="ACHJ7J_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO5001403.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGVFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(11491..12714)
                     /gene="gspF"
                     /locus_tag="ACHJ7J_003212"
     CDS             complement(11491..12714)
                     /gene="gspF"
                     /locus_tag="ACHJ7J_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO5001404.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPMHIEA
                     RMNASAGGMLQRRRHAHRRVASADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(12714..14207)
                     /gene="gspE"
                     /locus_tag="ACHJ7J_003213"
     CDS             complement(12714..14207)
                     /gene="gspE"
                     /locus_tag="ACHJ7J_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO5001405.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(14207..16267)
                     /gene="gspD"
                     /locus_tag="ACHJ7J_003214"
     CDS             complement(14207..16267)
                     /gene="gspD"
                     /locus_tag="ACHJ7J_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO5001406.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(16297..17256)
                     /gene="gspC"
                     /locus_tag="ACHJ7J_003215"
     CDS             complement(16297..17256)
                     /gene="gspC"
                     /locus_tag="ACHJ7J_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO5001407.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKVAHSLWRYFCFSAEYTVVSPSVNKPPRTDAKTFDKNDVQLISQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLVEEERSTVAVTNKKAISDEVKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(17274..17684)
                     /gene="gspS2"
                     /locus_tag="ACHJ7J_003216"
     CDS             complement(17274..17684)
                     /gene="gspS2"
                     /locus_tag="ACHJ7J_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO5001408.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(17750..18559)
                     /gene="pppA"
                     /locus_tag="ACHJ7J_003217"
     CDS             complement(17750..18559)
                     /gene="pppA"
                     /locus_tag="ACHJ7J_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO5001409.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(18886..>23367)
                     /gene="sslE"
                     /locus_tag="ACHJ7J_003218"
     CDS             complement(18886..>23367)
                     /gene="sslE"
                     /locus_tag="ACHJ7J_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO5001410.1"
                     /translation="GGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEPTPEPTPDPEPTP
                     EPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRVTGATCNGESSDGFTFKPGEDVTCVAG
                     NTTIATFNTQSEAARSLRAVEKVSFSLEDAQELAASDNKKNNAVSLVTSSNSCPADTE
                     QVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKAPSTHTSPVVPVT
                     TPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGYGVAGVNYYTNSGRGVTG
                     ENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQLIHRYSTTGQ
                     NNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGEQVVNLPNEFIEQFNTGQAK
                     EIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDEDYKSVTKFHVFHDST
                     NFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKNELAYITEAPSLV
                     EPENVTRDTATFNLPFISLGQVGDGKLMVIGNPHYNSILRCPNGYSWNGGVNKDGQCT
                     LNSDPDDMKNFMENVLRYLSNDRWLPDAKSSMTVGTNLDTVYFKRHGQVTGNSAEFGF
                     HPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGSDPYAIPLRADTSKPKLTQQ
                     DVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMALNKSVVNNDPQGY
                     PNRVRQQRATGIWVYERYPAVDGALPYTIDSKTGEVKWKYQVENKPDDKPKLEVASWL
                     EDVDGKQETRYAFIDEADHKTEDSLKAAKEKIFAAFPGLKECTNSAYHYEVNCLEYRP
                     GTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRLYQHELYFRTNGRKGERL
                     SSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNCYTNDAYTDGTQCSAD
                     LKKSLVDNNMIYGESSGKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNIKVDVEKYPG
                     AVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIKSNANVPVTVTVALAD
                     DLTGREKHEVALNRPPRVTKTYSLDASGTVTFKVPYGGLIYIKSDSKEEQSAEFTFTG
                     VVKAPFYKDGAWKNAPNSPAPLGELESDAFVYTTPKKNLEASNYKGGLKQFAEELDTF
                     ASSMNDFYGRDETSGKHRMFTYKALTGHKHRFTNDVQISIGDAHSGYPVMNSSFSTNS
                     TTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDRYLGKMNRVADDI
                     TVAPEYLEESNNQAWARGGAGDRLLMYAQLKEWAEKNFDIKQWYPEGSLPAFYSEREG
                     MKGWNLFQLMHRKARGDDVGNDKFGNRNYCAESNGNAADKLMLCASWVAQTDLSEFFK
                     KWNPGANAYQLPGVSEMNFEGGVSQSAYETLAALGLPKPQQGPETINQVTEHKMSAE"
BASE COUNT         5303 a         6580 c         5920 g         5564 t
ORIGIN      
        1 atggccccat gcaactggaa gtcccccgtg atcagtatgc cggcgctgtt gaaaccatga
       61 ggaataagat ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc
      121 tgattcgtcg gggacatctg acttatactc aggcccgtaa tatcacccgg ttcgggacca
      181 tcgaatcggt cacttatgat attgccgagg gttcggttgt cagtctggcg gccggaggga
      241 tcagttttgc cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc
      301 tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca
      361 caacccagca actccaccgg ctcagtgttg ttcagggtat gctgaagcac attgattttt
      421 cgactgccag cccgactgtc cgactggctc ttcagaaggg gaccggtgca ggaaatatca
      481 gtgccctgaa caaagtaatg aagggtacgc tggtgacatc tctggcactg gtagctgtca
      541 caaccggccc tgacatgatc aaaatgttgc ggggacggat ctccggtgcg cagttcatca
      601 ggaatcttgc cgtggcatct tccggtgtgg caggtggagc tgtcgggtca gtggcgggcg
      661 ggatactgtt cagtccactg ggaccatttg gtgcactgac agggcgtgtg gttggcggtg
      721 ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag
      781 aagatcgcgt caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt
      841 tcctgctgac cggacatgag attgaaaatc tgaacgagaa tctggcccgt gttatcgatc
      901 agaatgctct ggagatcatt ttcgccgccg gtatacaaca acgggccgcg accaatatgt
      961 taatcaaacc actggtggtc agtatcatca ggcaacgccc cgtcatggaa tatgatgcat
     1021 cccatctcgg caatatggtt aaccgactgg aagaagcatt acccccggag ttaccggcat
     1081 aacggctatt ctgcgccttt tacgctttat cacctgattt gtcagtgtca tcgtcatgac
     1141 tcagagaggt aaataccatg attcacctgt tcaaaacctg catgattacc gccttcattc
     1201 tggggttaac gtggtctgcc ccactccggg cacaggatca acgttacatc agtatacgca
     1261 atacagatac gatatggctc ccgggaaata tttgtgctta ccagttccgg ctggataatg
     1321 gcggaaacga tgaaggattt ggccccctca ccatcactct gcaactcaaa gacaaatatg
     1381 gtcagacgct ggtgaccaga aaaatggaaa cggaagcttt tggtgacagt aatgccacgc
     1441 gaaccacaga cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca
     1501 ttaaagcaac tgaagaaagt aacggccatc gtgtcagtct gccgttatcg gttttcaatc
     1561 cccaggacta ccatccactg ctgattaccg tttccggaaa aaacgttaac tgatccctga
     1621 agtcagtttc atgcttcagc ctctgtcaca acgcgtggca gaggcttttt atttatcagg
     1681 agaaaacaga tatgccgggt tacacagagt atgtcctggc tgaaggatct ttctcatacg
     1741 ggcaggcggt tgctgtcata accgcttacc gcaatgtgtt tatccaagat gacccgagaa
     1801 tgcatttccg tcgggttatc cgcagcgcgg aaggtcagcg acgctggcgc tgccggaatt
     1861 ctgaacccga tgccggaaag gtgcttaata cgcggctcgc cagtgacggt cttctcagac
     1921 aataaacgtc attcatttca cccatcaggc cgcgtcttct ccgggagacg tggctttttc
     1981 atttattaca ccagtaaatc ttaaccaccg ataaggagca aattatgcga ctagccagtc
     2041 gttttggtta tgcgaaccag atacgccgtg accgtccgct gacacacgaa gaactgatgc
     2101 actatgtgcc ggggattttc ggggaagata aacacacgtc ccgaagccag aactatacgt
     2161 acatccccac catcaccgta ctggaaagcc tgcagcggga aggctttcag ccattcttcg
     2221 cctgccagac ccgtgtgcgc gacccgggcc gccggggata cacaaaacac atgctgcgtc
     2281 tgcggcgggc cggagagata aacggagaac atgtccctga aattattctg ctcaactctc
     2341 atgacggtac ctccagctac cagatgctgc cgggttactt caggttcgtc tgccagaacg
     2401 gatgcgtctg tggtcagtct ctgggggaag tgcgtgttcc gcaccgggga aatgtggtgg
     2461 acagagtcat tgaaggggct tacgaggtgg tgggcgtgtt tgaccggatt gaggaaaagc
     2521 gtgatgccat gcagtcgctg attctgccgc caccggcacg ccaggcgctg gcacaggcgg
     2581 cactgactta ccgttatagt gacgaacatc agcccgtcac caccgccgac attctgacgc
     2641 cacgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc attcaggaga
     2701 atatgctgaa aggcggaatt tcaggtcgca gcgctaaagg aaaacgtatc catacccgtg
     2761 ccattcacag cattgacact gacattaagc tcaaccgcgc attgtgggtg atggcggaaa
     2821 ccctgctgga gagcctgcgc tgattcagcc attaccctga aaaccacgca tttatttgtc
     2881 ctttaaggag tcatttcatg aaaaccattt cacataattc caccacatct tctgtttccg
     2941 taaccactgc atcagggaat gaccagcctc agttggttgc caccatagtc cctgatgaac
     3001 agcgcatcag cttctggccg cagcattttg gcctcattcc acagtgggtg accctggagc
     3061 cccgtgtttt cggctggatg gaccgtctgt gcgaagacta ctgcggtggt atctggaatc
     3121 tgtacactct gaacaacggc ggggcattta tggcccccga accggatgac gatgatgacg
     3181 aaacatgggt actgttcaat gccatgaacg gcaaccgcgc tgaaatgagc ccggaagccg
     3241 ctggcattgc tgcctgtctg atgacgtaca gtcatcacgc ctgtcgtacg gagtgttatg
     3301 ccatgacggt ccattattac cggctgcggg attacgccct gcagcatccg gaatgcagcg
     3361 ccattatgcg catcatcgac tgaatggagg cagggacaat gcagcagctt tcctttctgc
     3421 ccggagaaat gacgcccggc gagcgcagtc tcattcttcg ggctctgaaa accctggacc
     3481 gccatcttca tgaacccggt gtggccttca cctccacccg tgcggcacgg gaatggctga
     3541 ttctgaacat ggcgggactg gagcgtgaag agttccgggt gctgtatctg aataaccaga
     3601 atcagctgat tgccggtgaa accctcttca ccggcaccat caaccgcacg gaagtccatc
     3661 cccgggaagt gattaaacgc gccctgtacc acaatgccgc tgccgtggtg ctggcgcaca
     3721 atcacccgtc cggtgaagtc acacccagta aggcagaccg gcttatcacc gaacgtctgg
     3781 tacaggcact gggcctggtg gatatccggg tgccggacca tctgatagtc ggtggcaacc
     3841 aggttttctc ctttgccgaa catggtctgc tttaacccgt cacaaccaca tcacacctgt
     3901 tttcactttt atcttctgtc ttcagaggta tcccattatg aaaattatca ctcgtggtga
     3961 agccatgcgt attcaccaac aacatccgac atcccgtctt ttcccgttct gtaccggcaa
     4021 ataccgctgg cacggcagca ctgaagcgta taccggtcgt gaagtgcagg atattcccgg
     4081 tgtgctggcc gtgtttgctg aacgtcgtaa ggacagtttt ggcccgtatg tccggctgat
     4141 gagcgtcacc ctgaactgat gttccgtcac ccggaaaacg agtacgactg gctgagctca
     4201 gacgccgctg tggatgaggc cctgctcata aacgaataca cctttaccga aggcggtctg
     4261 cgcgccggct gacctgtcat cgttaaacca ttcttaaccg attttctgtg aggattttat
     4321 cgtgtcagac gcactctccg ggacaatgct tcccgacgac aatcacgacc gcccctggtg
     4381 ggggctgccc tgcaccgtga cgccctgttt cggggcacgt ctggtgcagg agggtaaccg
     4441 cctgcattac cttgccgacc gcgccggtat cagaggcctg ttcagcgatg cagatgcgta
     4501 ccacctggac caggcctttc cgctgctgat gaaacaactg gaactcatgc tcaccagcgg
     4561 tgaactgaat ccccgccatc agcataccgt cacgctgtat gcaaaaggtc tgacctgcaa
     4621 agctgacact ctcggtagcg gtggttacgt ttatctggct gtttatccga cgcccgaaac
     4681 gaaaaagtaa accaccggaa taaccttctg ctccggcctg gtgctttcac cacgccactt
     4741 ttccattttt catctctgca tatcaggaaa accttcagta tgaaaacatt acccgataca
     4801 cacgtacggg aggcatcgag cagcccgtct cccgtcacca tctggcagac tctgctctcc
     4861 cgactactgg gccagcatta cggccttaca ctgaatgaca caccgttcgc cgatgaacgt
     4921 gtgattgagc agcatattga agctggcatt tcactgtgtg atgcggtgaa ttttctcgtg
     4981 gaaaaatacg cgctggtgcg taccgaccag ccgggattca gcacttgtcc ccgctctcag
     5041 ttaataaaca gtattgatat cctccgggct cgccgggcga ccggcctgat gacccgcgac
     5101 aattacagaa cggtaaataa cattaccctg ggtaagcatc cggagaaacg atgaaacttt
     5161 ccctgatgct ggaagccgac agaattaatg tgcaggcact gaacatgggg caaattgtcg
     5221 ttgacgtcga tggtgttaat ctcactgaac tgattaacaa ggtcgctgaa aacggttatt
     5281 cactccgcgt ggtggaggaa tccgaccaac agtcaacctg cacactacca ccgtttgcaa
     5341 cccttgccgg catacgctgc agtaccgcac atatcacgga aaaggataac gcctggctgt
     5401 actcgctgtc gcatcagacc agtgacgttg gtgaatcaga atggattcat ttcacaggta
     5461 gcggatatct gttacgtacc gatgcgtggt catatccggt tctgcggctt aaacgcctgg
     5521 ggctgtcaaa aacgttccgt cgtctggtta tcacacttac ccgacgttat ggcgtcagtc
     5581 tcattcatct ggatgccagc gctgaatgcc tgccgggttt acccactttc aactggtaac
     5641 caggaacaac atgaaatcat taaccacgga aaccgcactg gatattctga ttgcgtggct
     5701 gcaggacaat atcgactgcg aatcgggaat tatctttgac aacaatgagg ataaaacgga
     5761 ttcagcagca ctgttgccct gtatcgaaca ggccagagag gatatccgta ccctgcgcca
     5821 actgcagctt cagcaccaga accggtgagt ctcactcatc atctcactca ccagacttca
     5881 ttccactgac gccagcctga acacggctgg cgttttcatt tatctgcaaa aaggaatatc
     5941 gattatgtct gaaatcacag tctcccgtcc ggaagtggtc aacgagaata cggacgttat
     6001 ctgctccacc tcagtcaggt ccaggtcact ggaatatgat aattttccgg aaatcagcga
     6061 agcgaacatt ctgagcacat ttgaacaact gcaccagaac aaagatgaag tgtttgaatg
     6121 gggagtgatc aacgtcttca aagggctgag ctgggattac aaaaccaact caccctgtaa
     6181 atttggcagt aaaattatcg tcaacaatct ggtgagatgg gaccagtggg gatttcatct
     6241 tatcagtgga atgcaggcag atcgcctggc tgacctggaa agaatgttgc atctgctcag
     6301 cggtaaaccg ctccccgaca accgagggaa tatcaccatt aatctggatg accacataca
     6361 gtccgttcag ggtaaaggac gctatgaaga tgagatgttc atcattaaat actttaagaa
     6421 gggatctgca cacatcactt tcaaaaggct ggagctgatt gacagaatta acgatataat
     6481 agccaggcac tttccttctg tgctctcagc ctgaccccga gtttgattcc ctttcgatat
     6541 caaaagggac tgcgggtaca aaagagggta catatttcac caaaccaaac aaaataaact
     6601 aatatcaaca tgatagaagc attcttcgat tccgagtccg gcaccactaa ttcttaagaa
     6661 cccgcccaca aggcgggttt ttgcttttgg atctgacaat aaccttcacg aaaaaaatta
     6721 gcttataaag tctgggggaa ttactctcgc cacgttaacg agagtaattt tattgatatt
     6781 aatctcctga tactttaccc ccgtccaaac tccagccgct gcacattcac catcccaggc
     6841 ttctcagcag cactgacatc aatttgtgtc acccgcagcg catatttttc atccagtgcg
     6901 tttaaccatt tcagcaggtc attaaacacc acaggttcta tccagacctg aatattctcc
     6961 ccacgctcgg ctatccgcct gatgaccacc gagtgcgcgg aagcactgtc actgatgacc
     7021 cgcgatacct gcgcaggcgt tgttgtggca gattttcgcg ctgcaataat atccggcgcg
     7081 gcgttcttca gtcgggtgtt cattgccacc agctgctgca acatcgtctc ctgttgttct
     7141 atccgttcgc tcaacggctg ccagatgagg acgtaatatc cgacgctaaa caggaacacc
     7201 accgctgcca gtaacatgcc cttttcacgc ggagaacgcc ccgccaggtg ttgtgccagc
     7261 cagtgttcgc cacggcttaa ctggcgttca cgccattgct gaaaatagtg aataaattta
     7321 tcgcgtaaca tgttatttcc tccgcaacat tacgccgccg gaaaccgcat caccctcttt
     7381 ctgtaacgcg tcctgttgca caacataatc ggttgccagt gcactacgca gtttgtcgaa
     7441 actggcaaag tttgctgccc gcaactggag gtgaagcgtc tggcgttttt gatcaaaggt
     7501 gaaaccacgc atttcgatgt cggaaagtga cgctgatttc agggtgctgg ctatcgctga
     7561 caactcggcg agcagccggg tatcgtcggc ctgtgggcga tatttattca gcgccatcgt
     7621 cacctgagag cgtaaattca caatccgctt ctgctccggg aacaacgtta agaactgttt
     7681 ctcagcctgg tcgcggcttt gcgccacctg ttcgctgacg ctccataacg tcacgccccg
     7741 ttccactgcc agcgcaacca gaatcagcaa tatcggcaga atcatcactc gccagcgcgc
     7801 ccactgtttt cggtagctga cgcgaggctg ccacggccct gttagcaggt tcccttccgg
     7861 ttcgccataa gtggtaatgg ctggcagagc cgtaacggtc aggcgttcag cgtctgcgcc
     7921 agcccatgct gatagttctt ccggtgcaat gccgactaca gttagcgaaa gcggtaaatc
     7981 ctgctcattg agctgggcgc ggaacatgac cggagccagc gccagcccgg cgctccatcc
     8041 acggcattca tcgatgcggc aaataattcg ctgtgcatcg ccagccatat aaccacaagg
     8101 catggacatc cagtccggtg caacggtggc gcgggtgatg ccgtttgcct gcaaccactg
     8161 cgcaatgttg cgcatatgct gctggtgaat cactgccacg gttgcctgtt gctggtcgat
     8221 tttcaacgcg gcaaaatgca gttcgtcgat atcctggttc agctcttctt ccagcaaggc
     8281 gggcagaata gtcggtatct gcttgcgggc gacatcaggc agttcaacct gccagacgct
     8341 gaaccattcg ccgggaatgt agagtcgaat cgcatcagtt tgcagccatt gctggagacg
     8401 ttcgtcagca acgtcaggcc agataccgtg ctccacgtcg ggtgtacggc gctgccagcg
     8461 gatgggatca gtggtgcaaa gcggaaaaaa aatctcaagc atggaactca ctcactttct
     8521 cctgtctgat gccagagaac cgaaaagtgt tgtgggccca tgcggacaat taacgagttc
     8581 atcgtcagtt caatctcatt cacggtgata tctgaacgca gccagaagta attgctgtcc
     8641 acgctcagga cggtttttag ctgtttttta gtacgctcat cgacgtcagc aagtagcggc
     8701 tgtgcaagaa actgatcgac atcttcccag cccttcgccg gacgttgttg taataacgcc
     8761 cgcgcctgaa cagggcttaa ccacgggtca aacagcgcct caagaatcac actttgcgtg
     8821 acgtctaaag tattgatgtt gatttgctgg cgggtcatcg gcagcgcaca gaccagcggt
     8881 ttcagttttt gataaagccc ggcgtccatt ccctgcacca cgcgcatctc gctgatatcg
     8941 gccagcggtt gattggcggc gtagaaaggc accgagcggg cgagatattc gctgtcttca
     9001 cggcccagac gcgtctgcac gctgcggtct tcatcaataa actcccacag gctttcggct
     9061 atcagttcgg cccgataagc aggcacatcg aggcgcgaga tcagggcaat cagttgttgt
     9121 aacgcgagcg gacgcgacgc cgtcgtcggc tgagcgagga cattcaggtt aaagcacgcc
     9181 tgtgcgtcac gcagagtgac ggcgatttgc ccctgcggca gtgggaaaaa acgagggccg
     9241 gaagcccacg gctgtgccag atgcacgcgc ttttcatttt tcaggctcag actgagtgca
     9301 ctcagcgcca ggctttctgc actggcgctg taccacagcg cctgctggta ctcctgctgg
     9361 ctgcgggttc gcccaagttg ttgctgcatc cgcccggaaa gcgtgatggt taccagcatc
     9421 ataaccgcca gcaataccag caccacaacc agtgccattc cgcgttttgg tggtgaggtg
     9481 atcatgataa ttgcggcccg cgtaacaacc agatgcgttc aatctcgccc cattgcggcg
     9541 aatgcagggt catgcgcacc gccacaggga tcgcctgtac tgacgaccag ttctcctgcc
     9601 agcgcgtacc atcatagaac tgcaaacgga gcgaatcggc cggaatcaac ttttgcattg
     9661 tcggcttcac gctgcctgcg gcatcggtca gcggccaggc taaccgttcg agataaccgt
     9721 catgaatgcg gtaaccgacg gtgagcagat tgctgcgtgg cagacgcatc aacggattaa
     9781 ccacgccgcc gcgtacaaag cgcattcctt cactctcaga cgccagcacg ccagcgcccg
     9841 ccagtaacgc tggttctcgc tggccctgat cgcctcttac cggacgcggc atcatttggg
     9901 tcagatcgtg ggtcagaaaa ctcatcgttt gctgcatgag gtttagtttt tgatcgtgtc
     9961 cggcgacggc actattcacg cgtgtgacgc cgtttgtcac ctgctgcgtc atcagtgcca
    10021 gcgaggcaaa aatggcaatt gccaccagca tttccagtaa cgtgaaacca gcgcaagtcc
    10081 ttctcactgt tggcctccca cggcgctaaa ccaggcgcgt cgtgactgaa ttaccgacga
    10141 aaagtcttcg tgaaggctga cttcaatatc caccgcctgg agcagcgcgt tgcctgtatt
    10201 ctgcggtgtg gttcgccagt accagcggct tccagccata tcgctctcgc cctgatgcct
    10261 gaccgcccgt tgttcacggg tcatcaggta gagcagcgcg gtctggtttt cagccaccca
    10321 gcctgctacc gttttctcct caagaacgtg ctgattactc agcgcaccgc tggcaatctg
    10381 taacaccgcc gtggcagcca gcgcaaaaat cgccagtgcg aacatcactt ccagcaaggt
    10441 aaatccgcgc ttcatggcat cctctcatca cattgactga gggataacgc gccatcgtgt
    10501 gccagtttta ccgcccaaca tgcgttttgc gccgctgagt agaagcgcag cgtaaacggc
    10561 gtggcgggtt caaaaggcga aaaacggatc tgcggcgtct tctttttcgc ctctttttgc
    10621 aactccagtt caatatcgtg cagcgtcagg cgacgacgtt gcagctccag cgcatattcc
    10681 ttctgccaga tatcacttcc gggttgtaac agcatctgca cctgtttcgg caccgtaacc
    10741 tgtgccgata accgggtcgc agaaacgggc aaccactgcc cgtgacgacg ctgcataaac
    10801 tgatagccat gcgggtcgat taacacacca agtgtttgcc cttcgatcac tgccctgtcc
    10861 ttaaactgcg caaagcgagt cagaaaatcc tgcgccgctt ttttcgcggg cgattcagaa
    10921 tcggtcgcaa aagtctgtac cacgcccgca ctggcaaggc cgataaggaa aatcaccagc
    10981 atgatttcca gaagtgtgaa tccgcgttca ggcactgctt actgaaactc ctgcaaattc
    11041 cagttaccga tatctgcgcc agcgccttcc ccgttttcct gaccatccgc ccccagggta
    11101 taaacatcaa acactccttt ttcacccggg ctgagatact gataatcatt gccccacgga
    11161 tcctttggca gtcgtttaat gtatccacca gtacggtagt tgcgggcgtc cgccatattg
    11221 gctggctgct ggatcagcgc ctcaagcccc tgctcggtag ttggataacg cccgttatcc
    11281 agtcggtaca tatccagtgc gttttccagc gccacgatat cgctgatggc tttttgccga
    11341 tccgctttct ctttattgcc caacaggtta ggcaccacca gacttgccag gacgccaaga
    11401 ataacaatca ccaccatcac ttccagcagg gtaaaacctg cccgtggttt ttgtgtgcgg
    11461 gataacgaat tcatgtgata actccgtaaa ttacattcca accatattgt tcagttgcag
    11521 catcggctcg aggatggcga tgacgataaa cagcaccacg cccgccatca tcaccaccag
    11581 cgccggctca aacagcccta acgccagccc cacctgggta tcaaattccc gctcctggtt
    11641 aactgcggcc tgttcaagca tggtttccag ttcgccgctc tgttcgccgg aggcgatcat
    11701 gtacagcatc atcgggggga acaggcgcaa ctccgccagc gcagcgcgca gactgcttcc
    11761 ttcgcggacg cgatctgccg ccagcagcag ttgttgctcg acatagcgat ttgccgacac
    11821 agcggcagcg gtctgaatgc cttccagtag cggaacactg ctggcggtga gaatgcttaa
    11881 tgtgcgggaa aaccgcgccg tattcagccc gcgcgcaaca cgccccgtca caggcaggcg
    11941 cagcaaggtt ttatcccagc gcaggcgcat cgcaggattt ttgagtagcc gttgccccaa
    12001 caccagaagc gccagcaatc ccgccagcca gtaaacgccg ctggcctgta acgcgtcact
    12061 catggcgata agcgcgcggg tggtggcggg cagcgcgtgt ccgaggtgat cgaactgttc
    12121 gatgattttc ggcaccactg ccgtcagcaa aatagtgacc acgcccgttg ccaccaccag
    12181 cagaaccagc ggatagagca tggcctgcaa tagacgtgat ttcaggcgct gtcgctgttc
    12241 ggtgtaatcc gccaggcgat taagcaccac gtcgagatgc ccggattttt ctccggcagc
    12301 caccatcgaa caaaacaggg aatcaaagac gcggggatgt tcgcgcaggc tgtccgacag
    12361 ggtgtaacct tcctgaatcc ggctgcgcag cgccattccg aggcttttta catgcagttt
    12421 ttcactttgc tcactgaccg cctgtaagca ggtttccagc ggcattgctg cctgcaccag
    12481 cgttgccagt tggcgcgtga acagcgccag atctgccgac gccacgcgac gatgtgcgtg
    12541 tcgccgacgc tgcaacattc cccctgccga agcattcatc cgggcttcaa tgtgcatggg
    12601 aatgagatct ttaccgcgca acaactggcg ggcatgacgc gcggaatccg cctcaatcat
    12661 gcctttggtt ttgcgaccat tacgctccag cgcctgatag taaaacagtg ccattacgcc
    12721 tccatggtta cccgcagaac ttcatcgaga gaggtttctc cggcgagcac tttctcaatg
    12781 ccgttgctgc ggatgcccgc agagtgttgc cggacataac gttccagctc cagttccccg
    12841 gcctgacggt ggatcaaatc acgcaatgtg gcatccacca cgatcagctc atggatggca
    12901 gtacgtccgc ggaaaccttt atgattacag gcggggcagc cctgcggatg gtacagagtg
    12961 acggtacgcg cgtcggtaat tcccagcagg cgtttttctt cgtcagtggc aggcgcgggc
    13021 tggcggcagt cggggcacag cgtgcgaacc agtcgctgcg ccatcacgcc cgtcagactg
    13081 gaagagagca ggaaaggctc cacgcccata tcctgcaaac gcgtgatcgc ccccaccgcc
    13141 gtgttggtat gcagcgtgga aaggaccagg tgtccggtaa gtgaagcctg gacggcgatt
    13201 tctgcggttt cggtatcgcg gatttcaccg accatcacca catccgggtc ctgacgcaaa
    13261 atcgcgcgca ggccacgggc aaaggtcatg ccgacgcggg tgttaacctg tgtctgacca
    13321 attccttcaa tcatgtattc gatggggtct tcaaccgtga gaatattgcg cgaatggttg
    13381 ttcagctcct gcaatccggc gtacagcgtg gtgctttttc cggaacccgt cggccccgtt
    13441 accagaaaga tgccgtgcgg tttgtgtaac agctggcgca actgcacggt cagttgctga
    13501 ctcaacccca gacgttccag cgtcaggcgg gcctggtttt tgtccagcag tcgcagcacc
    13561 acgcgctcgc cccaggcgga gggcatggtg gagacacgca cgtcaatcgc ccgaccgccc
    13621 agcagcagcg caatacgtcc atcctgcggt acgcgctttt cggcgatatc cagccgtgcc
    13681 atcaccttga tacgcgatac cagcagcgag gccagtttgc gccccgggcg cagcatttca
    13741 tgtaatgtgc cgtcaacgcg aaaacggatc accagactct tttcaaacgt ctcgatgtga
    13801 atatccgaag cgccttcttt aatcgcctct gccagcatgg cgttgatcag tttgatgatc
    13861 ggcgcatcgt cgtcactttc cagcagatct tccgtttccg gcagttcttc agcgagagta
    13921 aaaaagtcct cagcagaacc gagatcttcc attagctggc gggcctcgga agagtcccgc
    13981 tgccagaccg cattcagtcg ctgttcaaat tcggcctcgt cgatttgccg cagcgtaaag
    14041 ggcgcgttca gcccccgctg cagctcctgc aaaacagaga gcgacaacgg atgcacatgg
    14101 aggatctcca gcgacgcttc gcaccatgcc accaggctaa accgacggct gaaactgtag
    14161 ggcagacgca cggtgttagc ggtggtttcc tgtgctacag gcaccattaa cgcgttctcc
    14221 cggcattaag gaacgcacgt acttccggcg gtaaggcctg gttttgcgct ggcagtaccg
    14281 gctgcgcggt gtgcggcatc aggcttaagc cttgctcatc acgatagatc tgttcggcgc
    14341 gcatatagtt atatttgcgc tgcgacacgc cgtctgccgc cataccgtca cgcagaatgg
    14401 tcggacggat aaacaccatc aggttacgtt tttctttttt atccgccgtc gatttaaaca
    14461 ggttaccaat caatgggata tcgcccagca gcggcacttt cgccacactt tctcccgcct
    14521 ggtcgtccat cagaccgcca agcacaatca gctcgccatc gttagccagt acggtggttt
    14581 tcagtttgcg ctcaccaaac accacgtcga ggctggtctg tccttccacc ttcgagactt
    14641 cctgctcaat caccatctgt accgcgttac cttcgttaat ctgcggcgtg actttcagca
    14701 tgatgccgac ttttttcctt tctactgtat tgaaaggatt gctgttattg gagccaacgg
    14761 tagagccggt taataccgga acgtcctgac ccaccatgaa gaaggcttcc tggttgtcca
    14821 gcgtggtgat gctcggcgtg gagagcacgt tagagctgga gtcgttttta accgcctgca
    14881 ccagcgccat ccagtcgcct ttcaccacac caaccgccgt accgctaaag ccggaaagaa
    14941 gctgggcgag cgtggagaga tcgccgttgg tatccggatt aatggtggta gcgccgtttt
    15001 cactgatcac cgtcgagcct ttctgcggtt tcgcctgaga aatggctgcc cccagcgtac
    15061 caatagggat ctgcgtacca ttagcaaact gcattaatcc ggcatctttc gacgcccact
    15121 gcacgccgaa attgatattg ctaccttcgg caacttccac gatcaacgcc tcgacatgca
    15181 cctgagcacg gcgaatatcc agttgttcaa tcacgctttg cagcgactgc atgatgtcct
    15241 gcggcgcggt tacaatcagg gcattactgt gtttgctggc ggcgatggag acaacctcac
    15301 gcccgctacc gaccgtgcct tccgcctctt ctttagccgc cgtgagcgtg ccgctgacct
    15361 gcttcagtac atcgaccaga tcttcggctt tgctgtattt gagatagaac acctggctgt
    15421 tgccgctgcg ctccatttct gagtccaggc gacggatcag gcggcgcatt ttgtcccgcg
    15481 tggccgggtc accactgaca atcacactgt tggtgcgttc gtcggcgaca atttgagatt
    15541 tcagcgtcgc aggctggttc tcgccgctgt ttttcgtcag gctttccagc acgcgggcga
    15601 tttccgaagc cgaggcgtta tccagcggga tcacctcttc ggtgcgatta cccgcgtgat
    15661 ccacgcgctg gatcacttcc gtcaggcgct ccacaacgga ggcgcgtccg gtgagcataa
    15721 tcacgttgga gggatcgtaa ttaacaacgt tgcctgagcc tgcgctgtcg atcatctggc
    15781 gcagaatcgg tgccagctcg cgtaccgaca cattacgtac cggcactact ttggtgacca
    15841 tttcatcgcc cgcgtagttg tcgctgcctt caccaaccag cggcagcggc tcgactttcg
    15901 cggcactgga tttcaccacc ttcagcacgt cgttttccat cggtacgacg gcatacccct
    15961 gtgcttccag caggttaagg aatagctggt aatactggcg ttcattgagc ggggtcatgg
    16021 tgcgaatact cactttcccc tgtacgcccg gccccataat gatggtttta ttaaggttag
    16081 cgccgacggt ttcgatgaac gatttcaggt cggtatcttt aaaattagcg gtgaaagtgg
    16141 cttcttccgc ccagaccggt gaactgcata atgccgctgc cagcaccagc ggcagtaaac
    16201 gcttttttgt tttgaggcca gttgtcttct tacgccagac cgacaacgta atatcacgcc
    16261 aaaacacgat gaatgattct cctggattaa atgcggttag cgcagcgcga tggaaatatc
    16321 gtggcgcgca cccttgcgta aaaccgtaag ttgaatggaa tccattgaag gtaactgccg
    16381 catcagagca atcattgctc gtggatcagt gaaatcctgc tgatttagtg caatggcgat
    16441 atcgccttcc ttaaaaccgc tggcatcgaa cagagaacga tctgcccccg gcttcactgc
    16501 ataaccgaca attccctcct tacgcacagg cgtaagctgg atatagttaa aaattttctg
    16561 cggatctttc gccagtgcct gacgcacggc agtcgggatc tcaactggcg cactgacagc
    16621 aggctcagca acagcttgct ttacttcgtc actgatggct tttttgttgg tcacggctac
    16681 ggtggaacgc tcctcttcaa ccaggctcag gcgctctatt tttccctgat agcgcagcat
    16741 cacatggtcg cggttgattt cctcaatcac cgcgttgtga gaaccaagcc gttccccctg
    16801 caaatagacc tgctgtttgc caccttcttc aataaccgcg ccgggtctgg caccaaaggc
    16861 gatcccgcgc agcaccacat taagacgcgt ttcggccaca ggtgcaggtt cgggttgttt
    16921 taccggtgcg gcgacgggct gatatttgcc aaaccagttt tgctggctga ttaattgcac
    16981 gtcattttta tcgaacgttt ttgcatccgt acggggcggt ttattcaccg atggggaaac
    17041 caccgtatat tccgcagaaa agcagaaata acgccagagt gaatgcgcca cttttgcaga
    17101 aataataagc agcatcagcc agaacatccc gcgtgcaatt tttcgcagat gctctttgtc
    17161 tgtattaaga gattgtctct ggttaagagt gtgacgaatt tttgtcagcc attgaatgag
    17221 ataaattcgt gcgtcacgaa aaacaacccg cgccaattta tatctacccg acgttatgct
    17281 ttgactattc cacaggtggt acgatccagt ttccgctgat actggttacc tgtacgtgta
    17341 tcattaatcg ttatgctgta attaattccc tcagtgagca ttaatttcac cgttgggtca
    17401 gcgcacattt gtcgttgata gctggttaaa aaggcgtcag gagtctgcgt ggtctgagta
    17461 cccgcttcgc tgataatggt catttttacc gtcgtgccgc tactttgtgc cagcactaag
    17521 gtatatcccg cagatttaat cggcaggttt tggctgatat tttgcgcctg ttttttcgcc
    17581 agtaaactgg cattttcatt atggccggca cagccactta ataatcctgt aacgctcaac
    17641 aatagcgata ttaataccct ccctggcatt tgttttatcg acatggtaaa taatctctaa
    17701 ggttattaat aagagttaaa atgtcacttt gataatgacg tggttatcat taaaacaatg
    17761 cctgtagata aagtgttgct ataccgccca gacttaaaca cggtccaaaa ggcagtgtgg
    17821 ttgatcctct ttttgtaata acggcatata tcaggccgca gcatgaggcg attaaagcaa
    17881 cattgggcag tgataacggt cccacccaac tacctaaagc ggcgaacaat aatacatcgc
    17941 ccatgcctaa tgcttcttta cgcagaacta ttccggctat ccagcgcaga gagtaaaaag
    18001 tgataaaccc caccaggacg ccggtgactg catcttgtag cgtgagcggg ctctgctgcg
    18061 cccatgccgc aattagtccc gtccacaata cgccctgagt aaaaacatcg ggcagccatt
    18121 ggtgatcgag gtcgatgatg ctcgcggcaa tcagccaggc ggataatatc atcaccgcca
    18181 gcgcccatcc actttccggc cacaccaggc tcgccagcaa aaaagcgagt gccgtcaata
    18241 actccaccag cggataacgc ttgctgattt tcgcctgaca atcacggcag cgccctttga
    18301 gcatcagcca ggagagcagc ggaatattgt cacgtatccg gatggtctgc tgacaatgtg
    18361 ggcagtgcga acgcggtagc gcaaggctta tttttgactg cgcactcggc atttcaccat
    18421 gaaactccgc catttgttgg cgcagcatga tggggtaacg ccaaatcacc acattcaaaa
    18481 aactgccgat gatcaatcct ccgacggtag ccagtatggg catcgccacg gggtattgct
    18541 gaaaaacatc aaaaagcatg gttaaaggtt gtttgttgta acttgccgga tgcggcgtaa
    18601 aacgccttat ccggcctaca ggtatctgcc aacgcaagta atcgcggctt tccctgaaga
    18661 cccgataaga tggcgcatcg ggcatatgtg ttaacccgat gcgccttatt tcatgccgga
    18721 tgcggcgcga gcgccttatc cggcctacag gtgtctgcca atgcaaataa acgcagcttt
    18781 ccctctctgt aggcccgata agcttgcgca tcgggcatat gtgttgaccc ggtgcgcctt
    18841 atttcatgcc ggatgcggcg cgagcgcctt atccggcata caggcttact cggctgacat
    18901 cttatgctcg gtaacctgat taatggtttc cggcccttgc tgcggtttcg gcagaccaag
    18961 cgccgccagc gtctcgtaag ccgactggct cacaccgccc tcgaagttca tctcgctcac
    19021 ccccggcaac tggtaagcat tcgcacccgg attccatttt ttaaagaact ccgaaagatc
    19081 cgtctgggcg acccaggagg cacacagcat cagcttgtcg gcagcgttac cgttggattc
    19141 ggcacagtag tttctgttgc caaatttgtc attgccaaca tcatcgccgc gtgctttacg
    19201 gtgcatcaac tggaacaggt tccagccttt catcccttca cgctcgctgt agaacgctgg
    19261 cagagagcct tctggatacc actgtttgat atcaaagttt ttctctgccc attccttcag
    19321 ctgcgcgtac atcagcagac ggtcacccgc accgccacgt gcccatgcct ggttgttgct
    19381 ctcctccaga tattccggcg cgacggtaat atcgtcagcg acacggttca tcttgccgag
    19441 ataacgatcc tgcatgtaca gcgccagcac gttgttcgcc acttcagttg cgcccggtac
    19501 agtcagcggc gtttctgcag cgttgtgccc tacttcgtgc cagatcagcc agtcgttcag
    19561 cggcgtcgtc ggcagcgtgg tgctgttcgt cgagaagctg ctgttcatta ccggataacc
    19621 cgagtgcgca tcaccgatgg agatctgcac atcgtttgtg aaacgatgtt tatgtcccgt
    19681 caatgcttta taggtaaaca tccggtgctt accgctcgtt tcatcacgac cgtagaagtc
    19741 attcatcgag ctggcaaagg tatccagttc ctcagcgaat tgtttcagac cgcccttgta
    19801 attgctggcc tcaaggttct tcttcggcgt ggtgtagacg aacgcgtctg attccagctc
    19861 gcccagcggc gcaggggagt tcggagcgtt tttccatgcg ccatctttat agaacggcgc
    19921 ttttactaca ccggtaaagg tgaattcagc tgattgctcc tctttactgt cgctcttgat
    19981 ataaatcaga ccgccataag gaaccttgaa cgtcaccgtg ccgctagcgt ccagagagta
    20041 cgttttagtc actcttggcg gacggttcag cgcaacttca tgcttctcac gtccggtcag
    20101 gtcgtcagcc agcgccacgg tgacggtcac aggaacgttc gcattggact taatggtgac
    20161 ctctttctga gccggagccc acaggccggt tgattgcatg ttacccgcaa accatttggt
    20221 cggattcgag tacaggctga tggtttcagt aacgttctgt ccctcttccg atactgctcc
    20281 cggatacttc tccacatcaa ccttaatgtt cagatcccac caggaacggc ccagcatcag
    20341 gcgcgtcagc ggtttttcca tatagttgag tggatagctt gggttcatca tgcccgcttt
    20401 accgctactt tcaccgtaga tcatgttgtt atcgaccagc gattttttca gatctgcgga
    20461 gcactgtgtg ccatcggtat aggcatcgtt ggtatagcag ttcaggaact cggtgaacgt
    20521 tttaaagccc agctcgtcat ttttgccttc ttcataacga tagctcgtat cgttccacag
    20581 ccagaccgac atgttctggt acagacgttc cagatcgacg ctgctcagac gctcaccttt
    20641 gcgaccattg gtccggaagt agagctcatg ctgatacaga cgctgaatgt tggtgcctaa
    20701 atccgcagcc tgcaccatcg cttttgccgt gtcggcgtta aggcttagtt gcgtatactg
    20761 tggaacatac atgccaccag taaccggaac ccccgtgcca ggacgatatt ccaggcagtt
    20821 gacctcatag tgatatgccg aattagtaca ctctttcagc cccgggaacg cggcgaaaat
    20881 tttctccttc gcagccttta gagaatcctc tgttttatga tcggcttcat caataaaggc
    20941 ataacgcgtt tcctgtttgc catctacatc ttccagccag ctggcaactt ccagcttggg
    21001 tttgtcatca ggtttgtttt ctacctgata tttccactta acttcccctg tcttactatc
    21061 gatggtgtac ggcagcgcac catctacggc aggataacgt tcatagaccc aaatgcccgt
    21121 tgcgcgctgc tgacgaacgc ggttcggata cccttgcgga tcgttattca ccaccgactt
    21181 gttcagtgcc atcgacagac ctgcggcatc cagcagacgc acaaaaccag acgcgctctc
    21241 ttccttaaga ttgctcatca cgttttccat gatcagcacc gatccacctt tgttcagata
    21301 ggcgatcaga tcggtcacat cctgctgagt cagcttcggt ttgctggtat ctgcacgcag
    21361 cgggattgca taaggatcac tacccacctg agtcacatat tcaaagccgt taaggatcag
    21421 cagcggcatt tcctgcgggt cgagatcgcc atagctactt aaatgttcaa cagagatacc
    21481 cgcaaaatcc ggatgaaaac cgaactcagc gctgtttcct gtaacctgac catgacgttt
    21541 gaaatagaca gtatccaggt tggtacccac ggtcatactg gattttgcat ccggcaacca
    21601 gcgatcattt gacagatagc gcagcacgtt ctccatgaag ttcttcatgt catccgggtc
    21661 gctgttgagc gtacactgcc cgtctttatt aacgcccccg ttccagctgt aaccgttcgg
    21721 gcaacgcaaa atgctgttgt agtgtgggtt accgataacc atcagcttgc catcgcccac
    21781 ttgccccagc gaaataaacg gcaggttaaa ggtggcggta tcgcgcgtaa cgttttccgg
    21841 ctcaacaaga gaaggcgctt cagtaatgta cgccagctca tttttatccc aggcccgttt
    21901 ctcgccgaag gccagccagt agtttttatc attacgcgcc atcagaatcg ggaaggccgc
    21961 gttggagata ttcaccaccg cctgaccgcg cgcattaccg gtgctgccat agaagttggt
    22021 agagtcatga aagacgtgga atttcgtcac cgatttgtaa tcttcatcca caccccacag
    22081 cttgttaata acgccctgaa tctggccgtc attaacatta cgcgtcgtca gcgagaacca
    22141 gcgagcctcg ttacaaccgt cggttttcgc acaaatcgcg gtatcgatct ctttggcctg
    22201 acccgtatta aactgctcaa taaattcgtt tggcagatta acaacttgct caccttcatc
    22261 cagcgtcgca ccgttggata acgagagatt gataatctcg ttgatcacgt tgggatattc
    22321 ggcaaagacc ttgcgtacat cgtccggcac aacacgggta ttattttgcc cggtcgtcga
    22381 atagcgatga ataagctgat caatattcgc cccgcgaact tcatcaccca gttcagtcag
    22441 cgcaatggtc gacttattgc cgcgcactga acccagttca aaggtatcga taccaaagga
    22501 gatggtttcg ccccagctaa aggaaaattc accattttcc cctgtcacgc cacggcctga
    22561 attggtgtag tagttgacgc cagcaacacc atatccctgg ctatccacca ggcgaccttc
    22621 tgagagaatg atttccgagg gttgatactg ataaaactgt tccgcgttag ccgacacgaa
    22681 ggaagcgttc agatccggtt ttgttcccgg cgtggtgacg ggcacgaccg gtgaagtatg
    22741 agtggatggc gctttatcgg tcgcagcatt gttttccacc tcttcattga ccagcttttt
    22801 gaactcttcc ggtgccagat cgatttgctt atacagcgag tcgaaacgtt tactctcgat
    22861 caccgaggag aacgtcaggc aaacctgttc tgtatccgcc ggacagctgt tactggacgt
    22921 taccagcgaa accgcattgt ttttcttatt gtcggaagcc gccagttctt gcgcgtcctc
    22981 aagactaaac gacacttttt caaccgcacg caggctacgc gcagcttctg actgagtgtt
    23041 gaaggtggca attgtcgtgt tacccgccac gcaagtaacg tcctcgccag gtttaaatgt
    23101 aaagccatcg ctggattcac cattacaggt agcaccagtt acccgctggc ttccgcccag
    23161 ggtcagataa cccgttttcg taggaacagg ttctggctcc ggttctggtg ttggttcagg
    23221 atcaggtgtc ggttctggcg taggctctgg gtccggcgtt ggctcaggcg tcggctccgg
    23281 gtttggtgtt ggatcaggtt tcacttccgg caaagaccct gttccagaat ctacaggcgg
    23341 cgtatcggag gaagatccgg aaccacc
//
LOCUS       DAZFFG010000075        23320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFFG010000075 DAZFFG010000000
VERSION     DAZFFG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.075"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            702..4514
                     /locus_tag="ACHJ7J_003219"
     CDS             702..4514
                     /locus_tag="ACHJ7J_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFO5001411.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GAAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGYRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINFGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESGALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTVDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLELVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDVAL
                     TANSVANNNATIEATGDLIIDSKGNVGNYHGKLHALNGVLSVKGSNLNNDYGEIRGYD
                     DVTLALTGNYDSFKGSLTSETGDVTLTANIIDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLTINAGGNIDNRDGNNFLRYNGEVFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLS
                     NASDGRLEDNTATGVIASDKNLDLNVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4603..6222
                     /locus_tag="ACHJ7J_003220"
     CDS             4603..6222
                     /locus_tag="ACHJ7J_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO5001412.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIVNRVELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTAVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGTSEYLSAKATRTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEVNYTRQIKNGYYNTAFFAQYAPRELTLQDQITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLMGAAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNINFNFSYQM"
     gene            6238..6606
                     /gene="acpS"
                     /locus_tag="ACHJ7J_003221"
     CDS             6238..6606
                     /gene="acpS"
                     /locus_tag="ACHJ7J_003221"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5001413.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTAHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6620..7381
                     /locus_tag="ACHJ7J_003222"
     CDS             6620..7381
                     /locus_tag="ACHJ7J_003222"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO5001414.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSMFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            7385..7933
                     /locus_tag="ACHJ7J_003223"
     CDS             7385..7933
                     /locus_tag="ACHJ7J_003223"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFO5001415.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            7955..8236
                     /locus_tag="ACHJ7J_003224"
     CDS             7955..8236
                     /locus_tag="ACHJ7J_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5001416.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            8272..9432
                     /locus_tag="ACHJ7J_003225"
     CDS             8272..9432
                     /locus_tag="ACHJ7J_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFO5001417.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISATELIASMNTILEKAEELDIQSLPEIQDMRENNWRAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTDEDLLIFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQSVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKAVFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            9506..12064
                     /locus_tag="ACHJ7J_003226"
     CDS             9506..12064
                     /locus_tag="ACHJ7J_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFO5001418.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSQARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSDATLPETASNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAAARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSHIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIA
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            12069..13292
                     /locus_tag="ACHJ7J_003227"
     CDS             12069..13292
                     /locus_tag="ACHJ7J_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFO5001419.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARKPWFTSDEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALEDLAAILPHGNGSAVSDNAEAKAITMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDCSVTNHSKRHLLKVGVGVDGS
                     VIGVVMSNRQAGRAK"
     gene            13289..14242
                     /locus_tag="ACHJ7J_003228"
     CDS             13289..14242
                     /locus_tag="ACHJ7J_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5001420.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGPFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEKDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMLSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHTQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            14253..14960
                     /locus_tag="ACHJ7J_003229"
     CDS             14253..14960
                     /locus_tag="ACHJ7J_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5001421.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            14944..15600
                     /locus_tag="ACHJ7J_003230"
     CDS             14944..15600
                     /locus_tag="ACHJ7J_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001422.1"
                     /translation="MTQMHKGFLLIGVFLYAAFLAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYDARHNVVALVFYAYGLLRQADSYAATNDFIHASEYAKSGFFWLD
                     EAVDLLEKNQRVRYLRARVDAYLPADSGRCVVTIKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLVQIKGQNTALAQSLMHDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            15601..16911
                     /locus_tag="ACHJ7J_003231"
     CDS             15601..16911
                     /locus_tag="ACHJ7J_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5001423.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKLRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMISNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGALAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16920..17708
                     /locus_tag="ACHJ7J_003232"
     CDS             16920..17708
                     /locus_tag="ACHJ7J_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFO5001424.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAARTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGVTQPENKQVLDISMRFMKPLGNEKADARSLVRFIYPPRDKGKVM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLVGEVSCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17705..19093
                     /locus_tag="ACHJ7J_003233"
     CDS             17705..19093
                     /locus_tag="ACHJ7J_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001425.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQPT
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFDEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLIDGSALLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            19107..20048
                     /locus_tag="ACHJ7J_003234"
     CDS             19107..20048
                     /locus_tag="ACHJ7J_003234"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5001426.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTPVAFL
                     EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            20045..21031
                     /gene="ycdU"
                     /locus_tag="ACHJ7J_003235"
     CDS             20045..21031
                     /gene="ycdU"
                     /locus_tag="ACHJ7J_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO5001427.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAEFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            21387..22591
                     /locus_tag="ACHJ7J_003236"
                     /pseudo
     CDS             21387..22591
                     /locus_tag="ACHJ7J_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         6137 a         5066 c         6075 g         6042 t
ORIGIN      
        1 tgatgtatag cggtgattgc tatcagatgg gaatgaaagc ttgccaaacc gttttgctct
       61 tctttctccc tttcaacgat aagccaggca taatgtggct gcttaagcca attgtcgcaa
      121 agccaaagat ctctatatag ctaacctcat ggttaatccg atctcagtaa taccatgaga
      181 aagaactatt tcctttttat gtaactctta agggttacaa gcgtgaatgc aaatggtaat
      241 ataatgaaat gtcgcctgcc cgtaatgacc ataaagaatc taccccgacg taaaagatat
      301 agagaataag atgcagtaat gatgatgctt tgttaatcaa ataacaagat ttaaattaat
      361 tgtaactcat caactttgtt gttttattcg gatgtctgaa atgatgtcgt acagaaaact
      421 acatatcatt tcagatatcc tctaaattgg atttttttca cccaaggagg aaggatggga
      481 aacttttaaa ttattgtgtt ttagtaagtt aggtacttcg ccgctcgatg aaaaaacaat
      541 aactcttagt tgttttttga gtccttattt cagcttattt ttcgtgattt tttattgcgc
      601 ctggtgatag gatttgccgc aatcattttt gctgatattc cgtgagtcat caaaagtgat
      661 ctgtatgtcc aaaatgaata gcatcaataa ggaaaacgtt agtggcgatg ataaatttaa
      721 gtaaggaagc gacggtgggt aaagcattaa cccctattgc tatacttatg atgttgtctt
      781 ttcctgtagc ttctcaagca gctggattag tcataaaaaa tggaacggta tataacgcca
      841 atggtgtgcc agtcgttgac atcaacaaac ctaacggtag cggtttatct cataatatct
      901 gggataacct aaacgttgat aaaaatggtg tcgttttcaa taacagcgct aatgaatcca
      961 gtacttcact tgccggaaat attcagggaa acagtaatct gacctccggg gcggcgaagg
     1021 tgatcctgaa tgaggttact tccaaaaatc cttcaaccat taatgggatg atggaagtcg
     1081 caggggataa agcggatctg attattgcca acccgaatgg tattactgta aacggtggtg
     1141 gttcaatcaa tacaggtaaa ctgaccttaa ccaccgggac gccggatatc caggatgaca
     1201 agctggccgg ttactccgtg aacggcggta ccattacgct cggtaaactg gataacgcca
     1261 gcccgacaga aattctgtcc cgtaacgtgg tggttaatgg caaagtgtct gccgatgagc
     1321 tgaacgttgt tgctggcaat aactatgtta atgccgcagg ccaggtgacc ggtagcgtat
     1381 ccgccacggg ttaccgtaac ggttacagcg tagatgttgc caaactgggc ggaatgtatg
     1441 cgaacaaaat tagtctggtc agcaccgaga aaggtgtagg ggttcgcaac ctgggcgtta
     1501 tcgcaggtgg ggttaatggt gtcagcatcg attccaaagg taatctgtta aacagtaacg
     1561 cccagattca gtctgcaagc acgatcaacc tgacgacaaa tggtactctg gataacacca
     1621 ccggtacggt gacatctgta ggcactatct cgctcaatac caacaagaat actatcgtga
     1681 atacccgtgc gggtaacatc tccacgatgg gcgatatcta cgttaacagt ggcacgattg
     1741 ataacaccaa cggcaagctc gccgcagcag gtatgctggc ggttgatacc aataacgcca
     1801 cgctgattaa ctttggtaaa gggagttctg tcgggattga agcggggctc gtggcgctga
     1861 aaaccggaac gctcaacaac agcaatggtc agattcgcgg cggttatgtg gggcttgaat
     1921 caggcgcgtt gaataacaac aacggtgata ttcagaccac cggcgatatc gccattatca
     1981 gtaacggtaa tgtggataac aacaaaggtc tgatccgttc gtccaccggg catatcgtta
     2041 ttggtgcggc aggtagcgta aataatggtt caaccaaaac cgccgatacc ggcagttctg
     2101 actctctggg cattattgca gataccggcg tagaaattgg tgcgaacaac atcaataaca
     2161 acggcggaca gattgcgtct aatggcaacg tctccctgtc aagttacagc acggtcgacg
     2221 attatgcggg caaaattctg tccaacagca aagtgattat caagggaagc tcactgcgta
     2281 acgataccgg ggggatcagc ggtaagcagg gtattgaagt cgccgttggc ggcagcctga
     2341 ccaataacat tggcgtgatc agctctgaag aaggtgatat ttccctgtta gccaactcag
     2401 tggacaacca cggcggcttc atgatggggc agaacatcac gatggagtcg atgtctggtg
     2461 tcaataacaa taccgcgctg atcgtggcca gtaaaaaact gaagataaat gcgcgcggca
     2521 gtatcgaaaa ccgcgatggc aataacttcg gtaatgctta tggtctgtac ttcggcatgc
     2581 ctcagcaaac gggtggaatg gtcggcaagg aaggcatcga gctttccggg cagaacatct
     2641 ataacaacaa cagccgtctt atcgctgagg atggtcctct gactctgcag gcgcagaaca
     2701 cgttcgacaa cacgcgtgct ctggtcacca gcggggcgga tgcatctatt caggttggcg
     2761 gaacgtatta caacaactac gctaccacct ggagtgcggg caacctggat atcgacgcga
     2821 ccacgctgca aaacagcagc agcggtacga tgatcgataa caatgcgacc gggtttattg
     2881 catctgataa aaatctgtca ctggaattgg tgaatagcct taccaactac ggctggatca
     2941 gcggtaaagg cgatgttgat gtcacggtga ataacggcaa cctgtataac cgcaatacca
     3001 ttgcggctga aaaggggctg gatattgccg cgttgaacgg tattgaaaac tggaaggata
     3061 tttctgctgg cggcgacctg acgatgaaca ccaatcgcca tgtgaccaac aactccaaca
     3121 gcaatatggt ggggcagaat atcgttatta acgcggttaa cgatatcaac aaccgtggca
     3181 acattgtcag tgacgctgac ctgaacgtga caaccaaagg caacctgtat aactatctct
     3241 atatggtggg ttatggggat gtcgcattaa cggcaaatag cgtggcgaac aataacgcga
     3301 ccatcgaagc gacaggcgat ctgattatcg attcgaaggg caacgtgggc aattaccacg
     3361 gtaagctgca tgcgttgaac ggcgtgttgt ctgtaaaagg cagcaatctg aacaacgact
     3421 acggtgaaat tcgtggttat gacgacgtca cgctggcact gacgggcaac tacgacagct
     3481 ttaagggctc gctgacctct gaaacgggcg acgtgactct gacggcgaac atcatagaca
     3541 acgcctatgg tctgattgcc ggtgagaatg tttctgtcga tgctaaatcg acgatttaca
     3601 acaacactgc gctgatcgcg gcgaataaaa agctaaccat taatgctggg ggcaacatcg
     3661 ataaccggga tggtaataac ttcttgcgtt ataacggtga agttttcggt attactgaca
     3721 acgttggtgg cattgtgggt aaagaaggtg tcacgctttc tgctcagaac gtctacaaca
     3781 acaacagcag tattatcgct gaaaatggtc cgcttaatct gctgtcccgg ggaacgctgg
     3841 ataatacccg cgcgcttctc agcagtgggg ctgatgccat tatccgtgcg gcagggacgt
     3901 tctacaataa ctatgccact acgtacagcg ccggtaatct cgacgtttat gcggcgtcgt
     3961 tgagtaacgc cagcgatggt cgtctggaag acaataccgc cacgggtgtg attgcgtctg
     4021 acaaaaacct ggatctgaac gtggataaca gcgtcacgaa ctatggttgg atcagcggga
     4081 aaggggatgt gcatttcaat gttctgaaag gcacgctgta taaccgtaat gccatcgcag
     4141 cggacaacgc gctgaccatt aatgccctga acggtgttga gaactttaaa gacatcgtgg
     4201 cgggaactgc gctgactatt gatacgcaga agtatgttac caacaacagc aacagtaata
     4261 tgttggggca aaccatcgcg atcaatgccg tgaatgacat taataaccgt ggaaatattg
     4321 taggtgatta ttctctgggg gttaaaacca ccggtaatat ttataactac ctcaatatgc
     4381 tgagttatgg tgtcgctggc gtatcggcaa ataaggttac gaatagcggt aaagacgctg
     4441 ttctcggtgg cttctacggt ttagcgttag aagcaaacga aactgataac accggtacta
     4501 ttgtcggcat gtaagatgta accaaagaca gcagtggctt ttaatattaa tgcgtcatgt
     4561 cacgcagggg ttgctgctgt ctttatttag tttgagaaga taatgcaatt acgatttaat
     4621 tttggtttgc tgctcattct ttgttgtttt caggttgcag cgaaagacag tcttgataat
     4681 ttgatcaagc agcaaaaaaa tgcagatgaa agcacgttac gcgcgcataa attagaaaaa
     4741 aaagatgttt actcaaatgc acaacgtaat gcgtttactc tgggtgatct gccgcaggaa
     4801 aagaattgct ttatcgttaa tcgggtagaa ctggaagata actttctggg tggtcagttg
     4861 atttctgaag tgcaaaaagc gattgccgga cgttgtgtcg gtagtgctgg tgtgcaaaaa
     4921 attgctgctg ccttgcagga ttattatatt aatgccggat atatcacaac gcgaattagt
     4981 attccttcgc aggatatttc taaaggcaag ctacgtttcc aggttaatgc cgggaagata
     5041 gaaaaaacga ttgtggaagg taatgacatc agtgaatgga tgttaccttt tcagcgtaac
     5101 gatattctca atattcgcga tattgaacag gggttggaaa atcttcagcg agtacctgac
     5161 gttgatgtga aaattaatat tgaaccggga acacgggatg gttacagcat cgttcatatt
     5221 gatacacacc gggggaaaaa ctggagcgtt cgggcaagtt acaacaattg gggcgatgaa
     5281 gaaacgggcc gttaccagac aaccgcggtg ggttatctgt ttaatccagc gaaaatgggg
     5341 gatctgtttt atctcgccgg tacgcgtagt actaccggac agtatgaaaa cgtcagtagt
     5401 tattattcca ttccggtggg ttactgggag tacggctttt tctatagcaa aagtaaatcg
     5461 cagcaggtga ttccgctttc gtacatctcg ctggattatg ttggcaccag cgaatacttg
     5521 agcgcaaaag cgacgcgtac cgtttatcgc gataaaacaa aaaagttcgc cggtagtgca
     5581 gaactcattc gaagaaagtc gaattacacg cttaatggtg aggagctgac attacaggcg
     5641 cgggatatgg gaaatatcaa gctggggcta aattataagc aacagttacc cggtgcgttc
     5701 tgggattcaa ccctctcgtg gcaacgcttt ttaacctggt tcggtggtga aaaaacgcca
     5761 gatatgcaat atggcgacgt tagcccggta agcaatatct ttaattttga ggttaactat
     5821 acccgacaaa taaagaatgg atattacaat actgcttttt ttgcccagta cgcaccgcgt
     5881 gaactgactt tgcaagatca aattacagtt ggcgatcgtt ggagtattcg cgggtttgaa
     5941 aatagcgtgg gtttgtcagg gaatgatggt ttttatataa aaaatacgct ggcatttcct
     6001 ttgcctggaa tgaaagctaa ctattatgcc ggtcttgatt ttgggcaagt gtatcaggac
     6061 gcttcttatg gcgatgaatc actgatgggc gcggcagttg gtattgatgg aaacattaaa
     6121 agtcttgagt ataacttttc cgtttctacc ccattaaagt atccggcgac attagatatt
     6181 gatcgggtga atataaattt taatttttcg taccaaatgt aaaggataac gagactaatg
     6241 agaataggga cagatattgt tgaaattgcc agaataacca aagccattat taatggagag
     6301 atggcttttt atgagcgtgt ttatactgct catgagcgct tactcattaa ccctgcagaa
     6361 cctgattatg aacgggcctc tggattctgg gcggcgaaag aagcgttagt taaagcgatt
     6421 ggttgtggtt ttcgtgatgg cgtacgtttc caggatattg aaataaagca tgatgcggcc
     6481 aaagcacctt atttcgttat ttccggaaga ctacaagaaa tttttattgg gcaggggttg
     6541 caaaataact ccctgagtat ttcgcattgc cgtacccacg ccattgcgac tgtcatcgtt
     6601 tattaactca acatatgtga tgaatatgta caaatataat gatttaaatg aaaaaaccgt
     6661 actggtgaca ggagccagtg gtgatattgg actgggtatt tgcgaaaaat atctggagca
     6721 gaactgcgat gtttatgcgc tctataaatc caacgacgtg caattaacgg cgctgaaagc
     6781 aagccatcca gcaggcgata aactgcatat tgtgcagtgc gatctcgcct gcccccagag
     6841 cgtgagcgca ttgtgcgaac agatagagcg ccaagctggc aagattgatg tgctggtgaa
     6901 taacgcgggc atcgttaaag atagcctgtt tgcctcgatg agctatgagg atttcaccca
     6961 ggttatcgaa accaacatgt tcagcatgtt ccgtctgacg aaagacgcgc tgatgctgct
     7021 tcgtgcggca gaaaatcccg ccattattaa cgttgcctct attgcagcgc tgatcccaag
     7081 cgtcggtcag gcgaattaca gcgcgtcaaa aggggcgatc ctcggcttca cccgcacgct
     7141 ggcagcagaa atggcaccct ggggcgtgcg cgtcaacgcg gtcgcaccgg gaatgattga
     7201 atcgaaaatg gtgaaaaaag tatcccgcgc agtggtccgt gcggtaacca gtaccatccc
     7261 gttacgccgt ctgggtaaat gtgaagaagt ggcgaacacc atcgtcttct taagttcctc
     7321 tgcctccagc tacattgtcg gccagaccat cgttatcgat ggcggcctgg tcatgcggtg
     7381 atttatgtct aaatacacga ttccttcgaa aatcttcctc gaaatgggcg gctggcgtca
     7441 gccgctgctg atggtcgata aaattgcaga ttataaatac ggtgaaaatg gctttgtcag
     7501 tgtcgtgaaa cacgtcacct ataacgaacc gtatctactg gggcatttcc cggaagatcc
     7561 gattatgccc ggggtgatta tttcggaaat cttcggccag gccagcgagt atctctcttt
     7621 cctgaccgac atttgcgata tctggcgcga acgttttgag gaagagctga agtcgttacg
     7681 tgatattcac gcacgcattc accgccctga aatgctggag attatccgta cccggcgcag
     7741 ccaggtacgc ggcgtgctgg ctgctcagaa cctgaaattt aaagatatcg cctatccggg
     7801 tgattctatt gatgttgtca gcaaattggc tttttcggat gccagtggct ttaaacacta
     7861 ttccgtgacc gcgtacgtcg gtaaaaagct gattagtcag ggcaccatta tcaatttccg
     7921 tgaaaccaag taatccaata agagagtaaa tattatgtct accatgaatg acgctattga
     7981 acagcgtaaa gaagttctga tgactattaa aactgagatc atccagcgac tgaatattca
     8041 acgcgatgaa gcgcaaattg atgatgatac tgcgctgttt ggtaatggtt ttaaactgga
     8101 ctccgtcgat gcaacggaaa ttgtcgttct gctggataaa gtatttgata tcaagatcag
     8161 cgaaagtgac gacccgtcct atatgcgcag tatcaatagt ctggccacct tcgttattca
     8221 aaagaaaaac gattaactac ttattaaaga attaattatc aggacacttt tatgaaatct
     8281 cttgctgaat ttcacttgaa aaataatgcg gtaatggggg tatataacaa ccgtacccta
     8341 ccttcttctt atcacgatgc tatgaccgaa tataaagccg ttcgtgaaaa cgcgctgctg
     8401 gtggactatt ctcacctctc tattgttagc gtcatgggcg atgatgcctg ggcgctgatc
     8461 aaccagctgg tttccgcaga tgtctccatc attcgtgatg agcaggcaat ctactcactg
     8521 gtgttgaatg aagagggcac cattcgcgga gacgtctatg tgctgtgcag catcgacggt
     8581 tattacctgc tgtctgaaga tatttcggct accgaactga tcgccagcat gaataccatt
     8641 ctcgaaaaag cggaagaact ggatattcaa tcactgcctg aaattcagga tatgcgggaa
     8701 aacaactgga gggcaatcct gctcgaaggc ccatactcct gggagatcat gtctgaaatt
     8761 catggctttg acgtgattgg cctgccttac tacgaataca tgaataccga tgaggatctg
     8821 ctgatcttcc gctgtggtaa acacggtgag tatgcgtaca tgaccatcgg tgagcaggcg
     8881 aaactggcgg agcagtggga aaaactgtta accgtcggcg agaaatacct gatgcagacc
     8941 ggcggcctgg attatcagaa aatcgtacgc ctcgaaaatc cgtgctggga tgccagcctc
     9001 tgggaaggtc agtcggtgaa tccggttcag ttgcagatgc agtgggcggt gcagtacgac
     9061 aaagatgatt ttattggcaa ggacgcggtg acagagctct cccaggagta taccggtaat
     9121 aaactcatcg gcatgatcgc acaggaagaa tgcgaaggca ttgaggctgg cgatcgcgtg
     9181 ctggtggaag gtcaagacgt gggttatgta gtgaaagcgg tattttcccc ggcattgcag
     9241 cgtttcattg ccctgaccct gctggaaaag gattacgcct ggtctgatat cagcggctac
     9301 gaaattcaaa ctgcccacgg aattattcca gcgcaatcaa aatgtatgcc gtttatttat
     9361 aacctgagca tgttggttag cccaactgag cacagttata tcgacgcgtc aaaaaataaa
     9421 agcgcagcgt aatgaatatc gccgcgtgaa tatagcttac gcggcttaaa ttaaccccaa
     9481 ttggcgggat tgccaggtat tattcatgaa aacgaacagg aaaagagttg ttattaccgg
     9541 gctggggatt ttgtcctcac tggcggaaaa tattcaggat ttccgggaag cattattaaa
     9601 taaaaaaaat ggcgttaagg atagcgaacg ctttgcaaaa tggtttgaaa atgcgcgtgc
     9661 ggcagaagtg ttgcatgaca ttgaatactc cgatctgaac gaagatgtta tcgattcact
     9721 ggataacgca gcgctctggg cctacaaagt gggtaaagat gcgctgagtc aggccggtct
     9781 gatggatagc cgtgacaccc tcaaagaaac aggcatgatc gtcggtgttt cgtccgcggg
     9841 aacagaggcc tttttgccgc tgttcgaaca gcgcatggaa gatttctcgc tgcgtaaagc
     9901 ccttttttcc ggcgcgttct catcctgttg ttccagcgtt tcgacgctgc tgggcttaca
     9961 gggtggggtt gaactggtgg cgaccgcgtg taccgccagc ccgaacgccg tggggatggc
    10021 ctttgactac atccagaatg gtaaaagcaa aaccatgctg gcagtgggca ctgagcccat
    10081 ctatctgccg acctttgccg gattctacgc actgaatgtc atgcatccag acacctgtac
    10141 gccgttctcg ggtcagtccg gtatgtcgat tggtgaaggc gctggcgcta tcgtgctgga
    10201 agagtatgag cacgccgtag cacgcggggc gaccatttat ggtgagatcc tctcttacgc
    10261 cacttcctgc gatgctttcc atgaaaccgg gccagatccg cgtgccagcg gagcggtgca
    10321 ggtcatgaac aaggcaatgg caaatgcggg tgtaacccca gagcaaatcg attacgtcaa
    10381 cgcgcacggt acaggtaccg aagccaatga ccgtatcgaa acgctggcaa tgaagaaggt
    10441 cttcgctaac cacgataaac tgctggtgag ctccaccaaa tcttactttg gccacaacat
    10501 tggcgcagcg gggattgtgg aactgattgc ctgtctggca acactgccgg acaaccgcgt
    10561 actgccgacg ctgaatttca gccaggcacg cccgaattgc gatctggact atgtgccgaa
    10621 tgaatttcgc gatcgcaaag tgaacatctt catgaagaat aactatgcct tcggcggcaa
    10681 caattgctgc atgatcctga gcatggagcc cgcgtccatt ccggtcagta cctatgattc
    10741 gaagcgtgtg gctatctcgg gcgtcggtgc agtatccgca ataggccaca cgctcaacca
    10801 ggttctggaa aacatctggg cgcaggacca ccacgttgaa ctgggtagcg taaccttccc
    10861 ggacgataca ctggaagaag ccaaagagct gctggccgtg ctggatgaat caaaccagtt
    10921 tgccgagctg tttgatgaag agtacagctt cagcgatgct acgcttccgg aaacggcaag
    10981 caacttcaaa acgttccagg tttccgggct ggagccgcgt aaacacctgc gtcgtttcga
    11041 tcagcgtaaa gcgacccgtg gcggcacgtt tgccctgatc gcactcaccg aagcgctgga
    11101 acaggcgaag cgtaaaatca aacgcgacgg cgatgagttg ggcatgatca tggggatgtc
    11161 ccgcggcccg caggaaacca cctacaaata tctgcaaagt ctgaaacccg atccacgcaa
    11221 agtacgtacg tcggaatttc ctggctcgct gatgaatgcg atcccaacgt tctgcgggat
    11281 ctctgaaggg atcaaagggt ataccactac gcttgcgaca ggggaaaact cggcgctggg
    11341 cgcgctgacc tacggttatg aaatcgtccg gcagaacctg caaccgcagg tattggttgg
    11401 cggcgcggat gagtacttcc cgtctatgtc gctgtatatg gatgcggtga cgcaaaagat
    11461 ccacatgacg gctgaggcga gcgactatca aatttacggt aaagatccgc agggttatgt
    11521 tccgggcgag ggtgcctgca tgctgctgct ggaagatcca cagttggctg cggcgcgtgg
    11581 cgctgaggtt ctcgcggaag tggtgggcta cggtaagtca tgtagtaata cctacttcga
    11641 cgcctcattg ttggaagaaa aatccgcgtc gatggcgctg gccattgaac gcgcccttac
    11701 gcatgcgggc atctctgctg gcgatattga tttggtatgt ggtaccagca acggcagcga
    11761 tgaccattct catatcgaaa ttgacgccat ttacaacacc ttcgccgcgc aaaacccggc
    11821 ggtgccggta gtgaactaca acgcttgctt tggttttgtc gcctctgccg ctggtttgct
    11881 taacctcgcc gtgattgctg actgcattaa gaagcaggca gtacctgcga ttccaaatac
    11941 ggtggaattc tttgatgagc gtgtgaattt tgtacgccag tcgatgaaac ttgcgctgaa
    12001 ccatgtcctg cttgttggcg caacggaagg cggcaactac tacgcgttcg tcattaaggg
    12061 ataagactgt ggacaatgcc gctattattt cgggttacag cgtgtggatg ccgtatgccg
    12121 aagactgttc gcagttaatt gacaatctga aacaggggaa acgcgttgcg cgtaaaccct
    12181 ggtttacctc cgacgaggaa gccattaaat gcgggtttaa gggtaatccg tcagtggcga
    12241 cgttgaaaca ggtggacgat agcgcattgg acctgctgtc tcagttgatc gacgaagccc
    12301 tggaacaggc aatgctggat aagcattgcc tggccgggcg caacgttcgc gtttatctga
    12361 ccggtattgg gccgcgtatc gacgggctgg attacaaatc tttctataat tacaacgatg
    12421 tagaagatat caacttaacg caatccatca caaatctgca tgcctcgaag atgtcgcagg
    12481 acacgatttc aagccatctt gcgcgcaaat atcgcttgca gtatttgccg cctaacatga
    12541 actgcaccag taactcgtca ctgactgcgg ttcatctggc gacgcagggc attgagcagg
    12601 gcgggattga tctcgctatt gtgctgaact gttcgaaaat taaaacccag gatatctggt
    12661 tcctggaaac gcaatcgatg ctggacagcg aacaagttca gccgtttggt gaaaacagta
    12721 aaggtgtggc gttcgcagaa gggtttagcg cgcttctgct ggaaagtgct catcaccgcc
    12781 gggcgcgtca acaaagtgag ggcgtaaggg tgcaaacaac ttacacccaa atcagcgcgg
    12841 gccgcagcaa tgatgcctcc tggcttagca ctaacgtgca caaggtcatg caggccgcga
    12901 tgaagcaggc tgaaattgcg ctggaggatc ttgcagccat tcttccacac ggcaacggtt
    12961 cggcagtgag cgataacgcg gaagccaaag ccatcaccat gttcgcaggg gagcggcaga
    13021 tccccgttct cgcctataaa gggcagatcg gctataccgc aaccggatct ggtgttgtcg
    13081 atctgatcat tggtcaccat tcgttgacgc atcatcaact gatcgcacct gttggcaacg
    13141 acgtcattat cgacagcatg gcttcgctgg tacttacgga ctgcagcgtg acaaaccata
    13201 gcaaacgcca tttgctgaag gtcggtgtgg gtgtcgatgg ttcggttatt ggcgttgtca
    13261 tgtcaaacag gcaagcgggg cgcgcgaaat gacggaaaag agtatctacc tgtcttcctg
    13321 ggcgcttacg gaaccggacg acatttttta tttttatcag ccgacctggc tcaacgcctg
    13381 ggaagcacat atgtacgaaa gaggattata cggcacccgt gctaatggct gggggctgaa
    13441 gcgctttggc gtggagaacg atatttatgc gccagaacgg atccgtaatc cgcaatatat
    13501 gccctacctt gaggcgatgg agcaggtgac cgattctgca ttacaacaaa tgccgactaa
    13561 agaacgcatg aagttaatga aatcgcgcac ggcatttatt tatgccgatt cctgggggga
    13621 atccggtccg tttgaaaaaa tttccagtgc gctgcatatc gccactattg atacgcttcc
    13681 caagaattta cttaaaaagt tttcgataac ggaaccgact tgcaagattc gcggcgaaaa
    13741 acaggcattc gttcaggcgc tgcgtatggc gcaggattat ctcgcctggg atgtattcga
    13801 taacgtggtg atttgtgctg cctatcgggc aattccggtg ctggtattct ctgaaaaaga
    13861 tgtgggtaaa ccgcctaagc gtttatttcc ccgtcgtgct gatgaggtga atttatctgt
    13921 tgaacgagtg ggttgtttta ttctcagcca gcgtgagagc gccatcaggt tgcaatgcgg
    13981 ggaatatcat tccggcactg cgccacagca tgctacggat attgatgtca tggccttcag
    14041 cgaacggcaa caaatgctgt cccgacctgc tgctggcgtt atcgacctgg tggcccatta
    14101 cggtgaaagc ggttgtctga cgcccgcgtt gagtatggat tatttaaccc gtcatactca
    14161 gcccggtgga aaaatgcgta cggtcattgc cgataaacag tttggttatc actactttga
    14221 ccttgaatac cttgaaggat aacgacagtc gtatgcaatc tactattaaa tcgttgcccg
    14281 ctatttcgct tagcaatatt tacaagtgtt atggcgctgg ggcaggaaag gttgatgcgc
    14341 ttaaaagcat taatcttgat atcaataaag gcgaatttct cgcactgtgt gggccgtcag
    14401 gcagtggtaa aagtactctg ttgaatattc tctcgggtat tgataaaccg accagcggaa
    14461 cggtgatatt tttaaataaa ttactgaacc aactgccaga agagcagttg gctgtgattc
    14521 gtggaaaaca tctgggtttt atttttcagt tttttaacct tatgccggta ttaaatgttt
    14581 tcgataacgt ttatttccca ctggtgctta atggtcagtt cagtaaaaaa gaagccactg
    14641 aacggacgct gcattatctc gatagcgtcg gattaagcgg ttttggcgat cgcaaacctg
    14701 gacaattatc gggcggtcaa caacagcgtg tcgccatcgc cagggcactg gcacacgaac
    14761 cgatggtggt cattgccgat gaaccgacgg gaaatttaga cctggcaacc ggcgaggcta
    14821 ttttggacct gctgttgacg attaatcagc agacaggcac cacatttgtt atttccacgc
    14881 actccagcga attaaaagcg cgggcacggc gagtggttga aattcaggat ggagtattga
    14941 ttcatgactc agatgcataa aggatttttg ctcatcggtg tctttttgta cgcggcgttt
    15001 ttggccccga agatttacgc ggcaacaccc gacgccgtta accaggttct gcaacagggg
    15061 ctcccggcgc agcctgccgc gctggcgacg ctgaccgagg cgttaaagaa agagtatgac
    15121 gccaggcaca atgtggtggc gctggttttt tacgcctatg gcttactgcg tcaggcagac
    15181 agttatgcgg caacaaacga ttttatccat gcctcagaat acgccaaaag cggctttttc
    15241 tggctggatg aagcggtgga tttgctggag aaaaatcaac gcgtacggta tttacgtgcc
    15301 cgcgtagatg cctatttacc cgcagattca gggcgttgcg tggtgacaat caaagatacc
    15361 gaacacatgc tggctgaccc ggcgatatgg acgacaacga tccgcgacca tattctggcg
    15421 atgcgttatc gcgcattacg tcactgcaag gacaccaccc gcgcgaatgc gttgttggtg
    15481 caaattaaag ggcaaaacac cgcgctagcg cagtcgctga tgcatgattt taatgttgta
    15541 ccagaatggg atagtgaaga gttaacccag gtactgctgc cgctgatgaa aggtgagtaa
    15601 atgtctgggg aaattttagc tgttaattgc gttctgctgc tgattctgtt ttttgtgttt
    15661 tatcagcttc tgttttattc acgagatgtc aaatttgcat ttttgaatct ctttcgccac
    15721 aagcgtcgct ctttttctac tataaccgcc attgtattgg gtggcgtggc aattttttta
    15781 tatggcggat ttattgatta ctctttctgg atcctgaaag agcagacgat tcgtaccaat
    15841 attggtcatg tacagattta caatcagcac tattttgaaa cgtcgaataa aaataagagc
    15901 ctgattgccg attatgcggc gttaaagaaa gctattttgt cggacccgac gctggcggcg
    15961 gatatttcca cgctctccgg gcaactggag tttactggcg taatttcaca ttacgagagc
    16021 gaaacctcca gctatttctc cgcgctgggg gtggagccgt tacccgcttt aaagctgggt
    16081 tcgttcgata agattatcgc ggggagcgat ctttcccggg taaaaggtga tgaaatcacc
    16141 ctgggcagcg gtctggcgaa aaccttgaat gcgaaatatg acgactggct ggatgtgatg
    16201 gtcgtcaaca cggcgggcgg gcagggcgca ttgtcgctaa aactgcgtgg catttttgag
    16261 tcaggcatta aagattatga cgatgtcgcg atgaagatcc cgctggacac cgcgcagcgc
    16321 atgatgggta cggatggcgt cagcaaagtg cttatcctgc taaaagagga tgacaccgcg
    16381 gcgtttagcg cgaagctgcg tcagtttatt gaacgcaatc atttgccgct ggtggtcaaa
    16441 gactggcgtg atgtttcact cttttatcaa caggttgagg ggttattgtc ggggatctat
    16501 ttcttcatca agctgattgt tgcgctgatt gtcatcttca tgatcagcaa ttcgatggca
    16561 atgaacattg ttgaacgcac ccgtgaaatt accactctgc gtgccatagg cctgaagccg
    16621 cttcacgtca cgcgcttgtt tctgacggaa ggcattttta ttggtgtgat tggcgcagtc
    16681 ggtagcctac tggtgggggg cgcgctagcc tggattatta atctttatgg tattgcgatg
    16741 ccgccatcgc cgggacaaac catcggctat accgccttta taaaaaccag taacccggaa
    16801 ttgatttggg tgacggtggt attacctatt ttgacagcaa caggtgcctc tgtgttaccg
    16861 gctttacgtg cttcccggct gaatatttct gacgcattta aattctcgta ggactgatta
    16921 tgaatatggc tttttacggc aagtggtttg cttgcctgtg gctcgctacg tcgtgcgttc
    16981 aggcggcgag aacagataat aaggcgctgg agataattcg tcgcgctgac gaaattcgtt
    17041 cgccgaataa accgtttcgt tacacgctga cggtaacgga atataaagcg ggcgtaaccc
    17101 agccagagaa taaacaggtc ctcgatattt ccatgcgttt tatgaaaccg ctgggtaatg
    17161 aaaaagcgga tgcgcgctcg ctggtgcgtt ttatttatcc gccgcgagat aaaggaaaag
    17221 tcatgctttc cgactggtac gatttatggt tttacacgcc ggaactgcga cgtccgatgc
    17281 ctatttcacg gcaacaaaga ttaattgggc aaatctctaa cggcgatgtc attgtcacta
    17341 actttgaata tgcctatgac tcgacccttg tgggggaagt ctcttgtgct gaaaagcagt
    17401 gttacaagct ggcgctggtg cgtaaatcgg cggatatcac ctggccgaag gttatctatt
    17461 acgttgaaaa agacggtgat aatcgcccgt ggaaggcggc ctattactcg caggatgacc
    17521 agttaataaa agaagtgttg tatcaggatt tccagccggt gctggggaaa acgcgaccca
    17581 tgaaaataac cgttaccgat gtccgtcacg gcaataacta ttcagtgatg gagtacagcg
    17641 atgttcgtct ggaatcgctg ccagagtttc atttcaccaa agagtatatt cagcgaggtg
    17701 cgaaatgaaa cgcacgctga agatctcttc gttgctgtgt gttgcgctgc cgttgacggt
    17761 acaggcggat tgcctgtcag gtgacgaggt ggcacaaaac agcgatgtga cgctctacga
    17821 gcaggtgtct tatgtcaata aacaggcttc ggcctggcag attgcaggca aaaatcccta
    17881 cacacgtcac aacggttatc aggaggcggg gattggcatc aatagtggtt gttcaataat
    17941 cgacaatacg ctggacctta agctcaatct gtatggaata aacgaatatg cgcttaaacc
    18001 cgcagggaaa ttcgaaacgg atgatagccg cacgcgtgcg ttaattaacc gcctcagtct
    18061 ggtctatagt gcctcggaca gcgttcaatt tgaagcaggc aaatttgccg cgccgtcagg
    18121 aatgttcttt ttgcgttcgc cttctgattt gcaaacgcat tattacaccg gattccagcc
    18181 gacgcgtttg cacgatccca aaatgacatc ggcgtatcag gcgtcatcgt ggggcgcaaa
    18241 aatgagcgtg gatacacgcg attatgcgtt ttcggcgagt gtcataccga agttagccac
    18301 catcgacaaa cgctatgtga cgtctggcaa ctggtcggct aatcagcagg gaaacagcga
    18361 cgaagcctat ctgctgagct tcagcgatca tcgctttgat gagcacacgc cgacagtcaa
    18421 tgtgcggctc ggaccatcgc cgtcactcgc gctgtctgac agttatcact acaccccgca
    18481 gcttacgctg aatgtcgacg cagcctacca ccgctcccag caatggcggc atctttccca
    18541 ccgtgagacg gcgcaggtcg aggaatatca gtttccggat tcgctctatg aaacgaaaga
    18601 tgaaagcggc gtggagctgg cgctgggtgg gcagtacacc agtgataatt tcagcgtgtt
    18661 tggcgttgag tattactttc agagcgaggg ttattcgcgc gctgaacagc gtcagcagcg
    18721 cgagttaatt gacttcctca ataccacaac cggctatgcg ccactggatc aggcatttga
    18781 ttcttacaaa tacctgatgg cttcagaaat cagcaatacc gccaatcagg ggatgctgca
    18841 gggtaagcac tatctgaacg cctgggccag tctgccgctg gcgggagagt cgacgctgca
    18901 gccttcgctg gtggtcaatc tcattgatgg cagtgcgttg ctgggcctgc attattccac
    18961 gccgctcagc gctatcagta accagcttga agcctatgcc ggtggctata gcgcgctggg
    19021 aagtcggtat tctgaatttg cgctttttgg cgacacgtta ggcctttatt tgggctttaa
    19081 atactatcta taaggataaa gagatgatga ataatgcttc acaaccgaca gtactgcttt
    19141 ttgccggtca gggtaatccg gttattggta tgggcagcga tctttgggat ctcaacgcca
    19201 gtacaaaaca tatctgggat tgtgccagcg atatttccgg actgaacctg cgtcgcctgt
    19261 gtcagaaagg gccgatgaat cgccttattc aaacgacggt acaacaactg gcggtgaccg
    19321 caattaatgt aacgctctat accctgtgcc gcgagcgtct tgagaattta acggttgtgg
    19381 gctcctgcgg acacagcgtt ggtgaataca gtgcgctcta cgccgctgga gctatttctc
    19441 ttgagacgct atttggcatg attcacttca gagccagcct gatgcatgac gaaagccaga
    19501 gaaataaagg cgcaatgctg gcggttaaag gcatcaaccg cgaacaactg cagacgatga
    19561 ttacggaatc aggcattgcg ctggatatta gctgtgataa cacgccgcgc cagcaggtta
    19621 ttgggggaac gcaggctgcg ttgaatgaat ttgccacgct gctgatggcc gctgggtacg
    19681 aaccggttaa actcggtgtg agcggtgctt ggcacacgcg tttgatggaa gacggcgttc
    19741 aggcaatgcg cgattatctt gccggactgg atatcgcttc gccagagcat caggttctga
    19801 tgaacgtaac agcaaaaagc gaggtcgctc cgtcaattat caaagaaaat ctctcattac
    19861 acttaactca tacggtgaaa tggactgaat ctctcgatac atttctgaat atgccaaccc
    19921 cggtagcttt ccttgaaata agtaataagc cctatttggg gaatatgtta aatgattttg
    19981 ctggcgtgga tcagcaacga gttatgcatt gtcgtaaagc atttagtgat gcgaaggtat
    20041 tcaaatgatt cctgattatt taacttttat tcgctttcag gataaacgaa atctgatata
    20101 catttatgct attggactta ttctgatagg cttttattgg aaaaatgcag ggtttacttt
    20161 tccatcagag gatattggtg tagttagtgg gattctggcc ctggtgctgt ataattttat
    20221 ttttgatctc aaggcgtact gggcttataa atgcgtcacg aagaatatcg atttttcgtg
    20281 gtttaagaaa aagcagaacc acaaaataga attatttctt acacaacctc tggtggcaga
    20341 atttctgtcg ttaatcatgt tgagtgcaat gagttggggg ctataccagc ttctaccctc
    20401 gttatatgcg ctgttcctga tttcgttact tgggccgttg gtcatctttc tgttgtttcg
    20461 gatgatccgc accagttatg tcaagcaggt cgctatttca gtagcgaaaa aagtaaaata
    20521 taaaagtctg actcgctatg tgctgctttc ggtgtgtatc tcaacggttg ttaacctgct
    20581 tactatcagc ccgttgcgta acagtgattc ttttgtgaca gaggggcagt ggttaacgtt
    20641 taaatcgata attgcattgc tcattctttg tggcgtagtg ttggcgatta atctattttt
    20701 tctgcgcttc tccaagcggt acgcttttct gggcaggctt tttttgcagg aaatcgatct
    20761 gtttttctcc agtgaaaatg cgttgtcgac cttttttgcc aagccgcttt ggcttcggtt
    20821 attcatattg ctggttattg aagtgatgtg gattacgctg gtgtcggtat tggcaacgct
    20881 tgtggaatgg cggatttggt ttgaagccta ttttttactc tgctatgtac cgtgcttaat
    20941 ttactatttt ttccattgtc ggttcctctg gcataacgat tttatgatgg catgtgacat
    21001 gtatttccgt tgggggcatt ttaataagtg aggaagttac aggaagtgac cagatagcgc
    21061 atatatgttc tgtactctct tgcgcatttt gattgttgac tgagcaatca gacagttgat
    21121 gtgcacgatt tcccctcgcc ctaacagacg tgggcggggg ctcccgatgt gtgcaatata
    21181 cgaaaaaaaa gcccgtactt tcgtacgggc tcttctttaa atatggcggt gagggggacc
    21241 gcctgaaata aatctgactt aatccagagg cgttgtataa tttaattata aacaatacat
    21301 tacaaatttt ttctgtccga tattatctaa tgtatgctta gtgaagtgtg agaataaggg
    21361 catactagag aaacaggaat tagcgtatgg cagtattgac tgatacaaaa gcaaggcata
    21421 tcaaacctga tgacaaacca ttgccccatg ggggaattac agggctgacc cttcatcctt
    21481 cttcagtaaa ggggcggggt aaatgggttt tttcgttatg taagtccggt gacacaaaaa
    21541 aggcgtaatg ctggattggg aacttaccct gaggtcagta ttgccgaagc tgcacgtact
    21601 gcccggataa tgcgagagca acttgctgca ggtgatgatc ctctggagat caaaaaggct
    21661 gaagctgaaa aagttgttat cccaacattt gccgatgcag ccatgcgtgt acatgcagaa
    21721 ttgtcaccgg gatgggaaaa tccaaagcat gtcaggcagt ggttgtcgac gcttgaaaat
    21781 tacgtgtttc ctcaactggg agcaaaaacg ctggatcaga ttacggctgc ggacgtggca
    21841 gagacactgc gtccaatctg gttgacctta tcagaaacgg caagccggat taaacagcgt
    21901 attcatgttg ttatgcagtg gggctgggcg catggttttt gtgtggcaaa tcctgttgat
    21961 gtggttgaac atttgcttcc acagcaatca agaggacgtg atgaacatca gccggcaatg
    22021 ccctggaggc aattaccgct gtttgtggcg acaagcgtgt atacagatga accttataat
    22081 gttacccgag cactgttatt aatggtgata cttacagcaa cctgttcggg cgaagcctgg
    22141 ggaatgcgct gggctgaaat tgattttcat aagcggatat ggacgatacc agcagaaaga
    22201 atgaaagata ggatacagca tcgtattcct ttatcccggc aggccattca cgttctggaa
    22261 aatatacgtg gtttgcatga tgaactggtg tttccttctc ccagaaagca gcagatcctt
    22321 tcagatatgg tgttgacgag ttttctgcgt aaaaagaatg ccatcagtga tatacccgga
    22381 cgggtggcaa cagcacatgg ttttcgttca acattcaggg actggtgtag tgaacaggga
    22441 tttggcggaa agggcgcttg cccacacgct gaaaaataag gttgaggcag catatcaccg
    22501 gactgatcta ctggatcagc gtataccgat gatgcaggcg tgggcggatt atgtgatgtc
    22561 acaacttccc agtaatccaa aaagatggtg aatcaagctc aagtgaccgg gctcagaatt
    22621 ccctctcagc aagggagagt gtggaaacac agagatactt taacggttgg tttttactgt
    22681 cgtgtcagag ctcgcagttt ttttgttgtc tgaacaaaag caataaaatc atttcaatag
    22741 ggtatagttg aagggggaag acgtcgtatg agtacgcgaa gattaatcag gataatggta
    22801 tatgagttgt tcggagacaa aagaagacat agagaaaatc tggctatacc atgaatatta
    22861 acaggcttta attctgcatt tattgagtca caatttctgc atgagaataa gagctcaatg
    22921 ggacgtataa tcatgacttg tatctggata gaggtaaatg atttaatggt atgcgttcta
    22981 tattggaaga ggggcgataa agcaaacagc gatattgtat atcagtttag gctctgtggc
    23041 tggagggtat ttattgtttg acgcatatgc ttagtggaga aataaaatta gagtaatgtc
    23101 ataaatgaga aaattcgctg tggttgtgta gaacatttaa tttcttcgtt tgtatgatgt
    23161 tggtgccata ttttttcagg ccttaaagtg gaagagggac gatttacggt gattgtagaa
    23221 acccgaagat atattatttc tttgtgcttg taatgtccgc cttgctagcg ctgcctgata
    23281 ataattgcac gtcgtataga tattggggca atagctgtat
//
LOCUS       DAZFFG010000076        23052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFFG010000076 DAZFFG010000000
VERSION     DAZFFG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.076"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(2..1651)
                     /gene="actP"
                     /locus_tag="ACHJ7J_003237"
     CDS             complement(2..1651)
                     /gene="actP"
                     /locus_tag="ACHJ7J_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO5001428.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1648..1962)
                     /gene="yjcH"
                     /locus_tag="ACHJ7J_003238"
     CDS             complement(1648..1962)
                     /gene="yjcH"
                     /locus_tag="ACHJ7J_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO5001429.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2163..4121)
                     /gene="acs"
                     /locus_tag="ACHJ7J_003239"
     CDS             complement(2163..4121)
                     /gene="acs"
                     /locus_tag="ACHJ7J_003239"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO5001430.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4514..5950
                     /gene="nrfA"
                     /locus_tag="ACHJ7J_003240"
     CDS             4514..5950
                     /gene="nrfA"
                     /locus_tag="ACHJ7J_003240"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO5001431.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5995..6561
                     /gene="nrfB"
                     /locus_tag="ACHJ7J_003241"
     CDS             5995..6561
                     /gene="nrfB"
                     /locus_tag="ACHJ7J_003241"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO5001432.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6558..7229
                     /gene="nrfC"
                     /locus_tag="ACHJ7J_003242"
     CDS             6558..7229
                     /gene="nrfC"
                     /locus_tag="ACHJ7J_003242"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO5001433.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTTGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7226..8182
                     /gene="nrfD"
                     /locus_tag="ACHJ7J_003243"
     CDS             7226..8182
                     /gene="nrfD"
                     /locus_tag="ACHJ7J_003243"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO5001434.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKLWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8202..9920
                     /gene="nrfE"
                     /locus_tag="ACHJ7J_003244"
     CDS             8202..9920
                     /gene="nrfE"
                     /locus_tag="ACHJ7J_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO5001435.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVMLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9913..10296
                     /gene="nrfF"
                     /locus_tag="ACHJ7J_003245"
     CDS             9913..10296
                     /gene="nrfF"
                     /locus_tag="ACHJ7J_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO5001436.1"
                     /translation="MSKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10293..10889
                     /gene="nrfG"
                     /locus_tag="ACHJ7J_003246"
     CDS             10293..10889
                     /gene="nrfG"
                     /locus_tag="ACHJ7J_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO5001437.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11231..12544
                     /gene="gltP"
                     /locus_tag="ACHJ7J_003247"
     CDS             11231..12544
                     /gene="gltP"
                     /locus_tag="ACHJ7J_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO5001438.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12734..13423)
                     /gene="yjcO"
                     /locus_tag="ACHJ7J_003248"
     CDS             complement(12734..13423)
                     /gene="yjcO"
                     /locus_tag="ACHJ7J_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO5001439.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13517..15664)
                     /gene="fdhF"
                     /locus_tag="ACHJ7J_003249"
     CDS             complement(13517..15664)
                     /gene="fdhF"
                     /locus_tag="ACHJ7J_003249"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15245..15247),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO5001440.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15862..17328)
                     /gene="mdtP"
                     /locus_tag="ACHJ7J_003250"
     CDS             complement(15862..17328)
                     /gene="mdtP"
                     /locus_tag="ACHJ7J_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO5001441.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAGPVVEKK"
     gene            complement(17325..19376)
                     /gene="mdtO"
                     /locus_tag="ACHJ7J_003251"
     CDS             complement(17325..19376)
                     /gene="mdtO"
                     /locus_tag="ACHJ7J_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO5001442.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQVLNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESKART
                     LPQKLAVALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19376..20407)
                     /gene="mdtN"
                     /locus_tag="ACHJ7J_003252"
     CDS             complement(19376..20407)
                     /gene="mdtN"
                     /locus_tag="ACHJ7J_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO5001443.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIHAGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20426..20701)
                     /gene="ytcA"
                     /locus_tag="ACHJ7J_003253"
     CDS             complement(20426..20701)
                     /gene="ytcA"
                     /locus_tag="ACHJ7J_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO5001444.1"
                     /translation="MPTVLSRMAMHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20910..22895)
                     /gene="yjcS"
                     /locus_tag="ACHJ7J_003254"
     CDS             complement(20910..22895)
                     /gene="yjcS"
                     /locus_tag="ACHJ7J_003254"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO5001445.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRADDYKFDINAAAPETI
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5191 a         6065 c         6269 g         5527 t
ORIGIN      
        1 attaatgcgc gcggccttgc tcaacgccaa agccggtctg ggagcggata aactgcgcgc
       61 ggaacagttc acgctcacgc gcgccttccg ctgagttatc ggttgccgag aagaaccaga
      121 tgccgaggaa tgccacgctg atagagaaca gcgccgggta ttcatacggg aagatagcct
      181 tttcgtgacc gaggatctgt acccaaatcg tcgggccgag gatcatcagt accactgcgg
      241 taatcagccc cagccagcca cccatcatcg cgccacgcgt ggtcagtttc gaccagtaca
      301 tagaaagcag aatgatcggg aagttacagc tcgccgcgat ggcaaacgcc agccccacca
      361 taaaggcgat gttctgattt tcaaacagca cgccgaggat aatcgcaatc acgcccaaaa
      421 tcaggacggt gattttcgat acccgcagct cttcacgttc ggtcgcacct tttttgaaaa
      481 cgttagcgta caggtcgtga gacaccgccg atgcgcccgc cagcgtcaga cccgctacca
      541 ccgcgaggat agtggcgaaa gcaaccgctg aaataaagcc gaggaacagg ttgccgccca
      601 ccgcattggc caggtgaact gctgccatgt tgttaccacc aatcagatgg cccgccgcgt
      661 ctttatattc cggattcgca ccgaccagca tgatcgcgcc gaagccgata ataaaggtca
      721 ggatatagaa gtagcccata aatccggtag cgtagaacac gctcttacgc gcttcgcggg
      781 catcgctgac cgtaaagaag cgcatcagaa tgtgcggcaa gcccgccgta ccaaacatca
      841 gtcccagacc cagagagagc gcggagatcg gatctttcac cagcccgccc ggcttcatga
      901 tgtcgacccc tttcgggtgt accgccatcg cttcgctgaa cagattgttg aagctaaagc
      961 cgacgtgttt catcaccata aaggccataa agctggcacc gaacagcaac agaacggctt
     1021 taataatttg cacccaggtg gtcgccagca tgccgccgaa caggacgtac atcatcatca
     1081 gcacgccgac cagcaccact gcaatgtgat agttaaggcc aaacagcagc tcgatcagtt
     1141 tacctgcgcc caccatctgg gcgataaggt aaagcgccac caccaccaga gaaccacagg
     1201 ccgaaagaat acgaatcggc ccttgtttca gacgataaga ggctacatcg gcaaaggtgt
     1261 aacgccccag gttacgcaga cgttcggcaa tcaggaacaa aatgatcggc cagcccacca
     1321 ggaagcccag cgagtaaatt aagccatcat agccggaggt aaacaccagc gcggagatcc
     1381 ccaaaaatga ggcggcggac atatagtcac cggcaatcgc cagcccgttc tggaagcctg
     1441 tgatattgcc gcctgccgtg tagtagtcgc tacgagaacg tacgcgtttt gatgcccagt
     1501 aagtaatgcc gagcgtaaac acgacgaaaa tcaggaacat aataatcgcc tgccagttcg
     1561 ttggctggcg ctctacggcc ccgctaatag catccgcggc gttagctgcg aaagggagtg
     1621 tggcggcaag cgccgtcaga actcttttca tgatgcttgt acctcatgca ggacttcgtt
     1681 attaagacgg tcgaattcgc cgttcgcccg ccagatgtag ataccggtga gaacaaagga
     1741 gatcacaatc actccaacac caatcggaat accgcgtgtg acgctggtgt tcggattcag
     1801 cggggtgccc agccagccgg gcgcgaaggc gatcagtaaa ataaagccga tataaactgc
     1861 cagcataata atcgacagga tggtggcaaa ccgttgccgt ttttcgacta actccctgaa
     1921 atgcgcattg tcttctatcc gctgataaat cgtaccattc atcacagatt ctccagaggt
     1981 aatgtaggga ttgttttaat tcccgctccc ttatgggaga aggttaacgc tcgggtaacc
     2041 cttgccgaat gtaggccgga taaggcgtta tacgccgcat ccggcaatca atgcctgatg
     2101 cgacgctgtc gcgtcttatc aggcctacaa accgttaccg actcgcatcc ggcaattgtg
     2161 ggttacgatg gcatcgcgat agcctgcttc tcttcaagca gcttctcgac tacgccagga
     2221 tcggcaagcg tcgaggtatc gcccaggttg ctggtatcgc ccgccgcaat tttgcgcaga
     2281 atacggcgca taattttgcc ggagcgggtt ttaggcaggg agtcggtcca gtgcagcacg
     2341 tctggcgtcg ccagcgggcc aatctcttta cgcacccagt tgcggacttc tgcgtacagt
     2401 tctggtgacg gttcctcccc gtgattaagc gtgacgtagg cgtagatcgc ctgaccttta
     2461 atattgtgcg gaataccgac gacagcggct tcggcgattt tcggatgcgc caccagcgcc
     2521 gactcaatct ccgccgttcc cagacggtga ccggagacgt tcagcacatc gtccacacgc
     2581 ccggtgatcc agtaatagcc atcttcatca cgacgcgcgc cgtcgccgct gaaatacata
     2641 tttttgaagg tggaaaaata ggtctgctca aaacgttcgt gatcgccaaa cagcgtacgc
     2701 gcctgacccg gccaggagtc ggtgatcacc aggctacctt cggtagcccc ctccagcggg
     2761 ttaccttcgt tatcgaccag cgccggttgc acgccgaaga acggacgtgt tgccgaaccg
     2821 gctttcagct cggtagcgcc aggcagcggg gtgatcatga aaccgccggt ttcggtctgc
     2881 caccaggtat cgaccaccgg acatttctcg ttgccgattt ttttccagta ccactcccac
     2941 gcttccgggt taattggctc gcccacggaa ccgagaatgc gcagcgacga acggtcggtg
     3001 ccttcgatcg ctttatcgcc ttccgccatc agcgcgcgaa tcgccgtggg cgcggtatag
     3061 agaatattga cctgatgctt gtccaccacc tgtgccatac gggcaggcgt cggccagttc
     3121 ggtacgcctt caaacatcag cgtggtcgcg ccgcaggcca gcgggccgta cagcaaataa
     3181 ctgtgtccgg tcacccagcc cacatcggcg gtgcaccagt agatatcgcc cggatgataa
     3241 tcaaagacat atttaaaggt cagcgccgcg tacaccagat aaccgccggt agtgtgcagt
     3301 acgccttttg gttttccggt agaaccggag gtatagagaa taaacagtgg atcttcggcg
     3361 ttcatctctt ccgcctggtg ctgatcgctg gcttgctcaa ccaggtcgtg ccaccacagg
     3421 tcgcgccctt cctgccagtc aattttcccg ccagtacgct tcagtaccac cacatgctct
     3481 acgctggtga cgttcgggtt ttttagtgcg tcatcaacgt ttttcttcag cggaatacta
     3541 cgcccggcgc gcacgccttc gtcggaagtg atcaccaacc gtgagttaga atcgataatg
     3601 cgcccggcaa cggcttccgg cgagaagcca ccgaaaatta ccgaatgcac cgcgccaata
     3661 cgggcgcagg ccagcatcgc aaccgcggct tccggcacca tcggcatata aatcgccacc
     3721 acgtcacctt ttttaatgcc cagctcgagc agagtattgg cgaagcggca gacgtcgcgg
     3781 tgcagctctt tatagctgat atgtttgctc tggctggcgt cgtcgccttc ccagatgatg
     3841 gcggtacgat cgccattttc ttgcagatgg cggtcaaggc agtttgccgc cagattcagc
     3901 gtgccgtcct cgtaccattt aatggacaca ttaccggggg caaaggaggt gtttttcacc
     3961 ttctggtacg gtttgatcca gtcgagaatt tttccctgtt cgccccagaa ggtatcaggt
     4021 gcgttaatag attgttgata catcgcctcg tactgctgag ggtttatcag gcaacggtct
     4081 gcgatgttgg caggaatggt gtgtttgtga atttgactca tgcttttgtt ctccttgtag
     4141 gatgttaata atatgtggca taagcgttaa atgtaggggt attggcagtt ttgtttagta
     4201 tttgggcgac agatcacgca aaagtagaat tgtgcaaata aacggcaggg taatttttga
     4261 aggtcagcaa caaaagttga ttaattcttt gaggaacatg cagttatgca tgctgttgaa
     4321 aaagaggaag atactgacta actctaaagt ggtattttac atgcacttac aattgattaa
     4381 agacaacatt cacagtgtgg ttatttgtta cacatagggg cgagcaatgt catgacagtg
     4441 taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc
     4501 ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc cgtatcttca gcttattgat
     4561 tccttttttc tttttcactt ctgttcacgc tgaacaaacg gctgctcccg caaaacctgt
     4621 aactgtggaa gcgaagaatg aaacctttgc cccgcagcat cccgatcaat atctctcctg
     4681 gaaagccacc tcggaacagt cagagcgtgt tgacgccctg gcggaagatc cacggctggt
     4741 gatcctgtgg gcggggtatc ccttctcgcg cgattacaac aagccgcgtg gacatgcctt
     4801 tgctgtgacc gatgtgcgtg aaaccctgcg taccggtgcg ccgaaaaacg ctgaagatgg
     4861 tccgctaccg atggcgtgct ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa
     4921 agacggcgaa gatggctact tccacggtaa atgggcgcgc ggcggcccgg aaatcgtcaa
     4981 caacttaggt tgtgccgact gccataacac cgcctcacca gagttcgcca aaggcaaacc
     5041 ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt
     5101 tgagaaagcc ggacgtttcg accagcaatc gatggtttgc ggtcagtgcc atgtggagta
     5161 ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg gatgacggca tgaaagtcga
     5221 aaatatggag cagtattacg acaaaattgc cttctctgac tggactaact ccctgtcgaa
     5281 aacgccaatg ctgaaagcgc agcacccgga atatgaaacc tggacagcgg gcattcacgg
     5341 taaaaacaac gtgacctgta tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa
     5401 actctacacc gaccataaaa ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa
     5461 ctgccatacc caggacaaag ctgccttgca aaaagtggtc gcggaacgta agcagtcgat
     5521 taacgacctg aaaatcaagg ttgaagatca actggttcac gctcacttcg aagcgaaagc
     5581 ggcgctggac gcaggtgcga cggaagccga aatgaagccg attcaggacg atatccgtca
     5641 tgcccagtgg cgttgggatc tggcgatcgc ttcccacggc attcatatgc acgcaccgga
     5701 agaaggtctg aggatgctcg gtacggcgat ggataaagcg gcggatgcac gcaccaaact
     5761 ggcgcgcctg ctggcgacca aaggcatcac ccatgaaatc cagatcccgg atatctcgac
     5821 caaagagaaa gcccagcagg ccattggcct gaacatggaa caaatcaagg ccgagaagca
     5881 ggacttcatc aaaacggtga tcccgcagtg ggaagagcag gcacgtaaaa acggtctgtt
     5941 aagccaataa ccccgttccg cctcgcaagg ggcggaaaac acaatggagt gaatatgagc
     6001 gtattacgtt cgttattaac tgccggggtg ctggcgtcgg gcctgttgtg gagcctgaac
     6061 ggaattaccg ctacccctgc ggcgcaggca tctgatgatc gctacgaagt tacccagcag
     6121 cgtaacccgg atgccgcctg tctggactgt cataaaccag ataccgaagg tatgcatggc
     6181 aaacatgctt ccgttatcaa cccgaataac aaactgccgg tcacctgcac caactgtcac
     6241 ggccagccat caccgcaaca ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg
     6301 atgtacaagg ttggagagca gaacagcgtc tgtatgtcct gccatctgcc ggaacagttg
     6361 caaaaagcgt tctggccgca cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat
     6421 tccctgcatc cacagcaaga tacgatgcag acgttaagtg acaaaggacg gattaagatt
     6481 tgcgtcgatt gccacagcga tcagcgcacc aatccgaact ttaacccggc gtctgttccg
     6541 ttgcttaagg agcagccatg acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc
     6601 tggcagccgt cagcggcacc acagggcgcg tggtggcgaa aacgttgaat atcaatgggg
     6661 tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg
     6721 cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg
     6781 agccgcaggg cgaatttcct gatgtgaaat atcgcttctt ccgtaagtct tgccagcact
     6841 gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta
     6901 gcgggattgt cgatgttaat cctgatctct gcgtcggttg tcagtactgc atcgccgcct
     6961 gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac ggcggacaaa tgcgatttct
     7021 gccgtaagac caatttgcag gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga
     7081 aggcgctgac gtttggcaat ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc
     7141 agaagcctac ttaccgctac aagctggcgc tgggaaccaa accgaagctg taccgcgtac
     7201 cgtttaaata cggggaggtg agccaatgac gcagacttcc gcatttcatt ttgaatcgct
     7261 ggtgtgggac tggccgattg ccatctacct gtttttgatt ggtatttctg ctggtttggt
     7321 gacgctggct gtgctgttac gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt
     7381 gctgcgcacc acgctgattg tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt
     7441 cttccacctg acaagaccgt ggaccttctg gaagctgatg ttccactaca gttttacctc
     7501 ggtgatgtcg atgggggtga tgctgtttca gctttacatg gtggtgctgg tgctgtggct
     7561 ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa cgctggttgc cgaagctggg
     7621 gatcgtgcaa aaggttctga gcctgctgac gcccgttcat cgcggactgg aaacattgat
     7681 gctggtgctg gcggtgctgc tgggggctta taccggtttt ctgctgtcgg cgctgaaatc
     7741 gtatccgttc cttaataacc cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc
     7801 cggtgcggcg gtggcactga tcgccatggc gatacgccaa cgcagtaacc cgcattccac
     7861 ggaagcgcag tttgtacacc gtatggaaat ccccgtggta tggggtgaaa tcttcctgct
     7921 ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt aaagtgcgtg cgctggtggc
     7981 ggcattgggc ggcggtttct ggacgtggtg gttctggctt ggtgtcgctg ggctggggct
     8041 gattgtgcca atgttgctca aactgtgggt caatcgcagt tccggcattc ctgccgtgct
     8101 ggcggcgtgt ggggccagtc tggtcggcgt gttgatgttg cgctttttca ttctctacgc
     8161 cgggcagtta acggtggcgt aagccagaac agaggtggtt tctggacgta ttccttcctg
     8221 aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg
     8281 ccttcgcggg agtgcggttg cgctggcctg ccatgatgcg actcacttgc atcggcattc
     8341 tggcacagtt cgcgctcctg ctgctcgcct ttggcgtact gacgtattgt tttctcatca
     8401 gcgatttctc ggtcatttat gtcgcccaac atagctacag cctgctgtcg tgggaactca
     8461 aactggcggc ggtatggggt ggtcatgaag gttcgctgct gctttgggtt ctgctgcttt
     8521 ccgcctggag cgcgctgttt gcctggcatt atcggcagca aaccgatccg ctatttccgc
     8581 tgacgttagc cgttttatct ctcatgctcg ccgcactgct actgtttgta gtgctgtggt
     8641 ccgatccctt cgtgcggata tttccaccag caatcgaagg ccgcgatctc aatccgatgc
     8701 tgcaacatcc cggtcttatc tttcatccac cgctgcttta ccttggctat ggcggtttga
     8761 tggtagcggc gagcgtggcg ctggcgagtc tactgcgcgg cgagtttgat ggtgcctgcg
     8821 cccgaatttg ctggcgctgg gcgttacctg gctggagtgc attaactgcg gggatcatcc
     8881 tcggttcctg gtgggcctat tgcgaactgg gctggggcgg ctggtggttc tgggatccgg
     8941 tggaaaacgc ctctttacta ccctggcttt ctgccactgc gttgctgcac agtttatccc
     9001 tgacacgcca gcgggggatt tttcgccact ggtcgctgtt actggcgata gttactctga
     9061 tgctgtcgct gctgggcacc ttaattgtcc gttctggcat tctggtttcg gttcatgcgt
     9121 tcgcgctgga taacgtccgc gccgtgccgt tgttcagtct gtttgtactg attagccttg
     9181 cgtctctggc tctgtatggc tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg
     9241 ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct gttttgcgca gtgctactga
     9301 tcgtgctggt gggaacgctt tatccgatga tttacggcct gctgggctgg ggacgcctct
     9361 ccgttggcgc accgtatttt aaccgcgcga cgttaccgtt tggtctgttg atgttggtgg
     9421 tgattgtgct ggcgacgttt gtctctggta aacgcgcgca gcttccggcg ctgctggcgc
     9481 atgcgggcgt gctgttattt gccgcaggga tcgtggtttc cagcgtcagc cgtcaggaga
     9541 tcagcctgaa tttacagccg ggccagcagg tgatgctggc aggatacacc ttccgttttg
     9601 agcgcctcga tctacaagcc aaaggcaatt acaccagcga aaaagcgata gtggcgctgt
     9661 ttgaccatca gcaacgcatt ggtgaattaa cgccggagcg gcgtttttac gaagcacgtc
     9721 gtcagcaaat gatggaaccg tcaattcgct ggaacggcat ccatgactgg tatgcggtca
     9781 tgggggagaa aactgggccg gatcgttacg cttttcgttt gtatgtacaa agcggtgtgc
     9841 gctggatctg ggggggagga ttgttgatga ttgcaggcgc attattaagc ggatggcggg
     9901 ggaggaagcg cgatgagtaa aggacttctc acgctactgc tgttatttac ctgttttgcg
     9961 cgcgctcagg ttgtagacac ctggcaattc gctaatccgc aacaacagca acaggcgtta
    10021 aatattgcca gccagttacg ttgtccgcag tgccagaatc aaaacttact cgaatccaac
    10081 gcgccggtgg cagtcagtat gcgccatcag gtttacagca tggtggcgga ggggaagaac
    10141 gaagtcgaaa tcattggctg gatgaccgaa cgctacggag attttgttcg ctataaccca
    10201 ccgttaacgg gtcagacgct ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg
    10261 gcactgatcc tctggcgagt gagggcgaag cgatgaaaca gcctcaaata ccggtgaaaa
    10321 tgctgacaac actcacgata ttgatggtat tcctctgtgt cggcagttat ctgttaagcc
    10381 caaaatggca ggcggtacgt gcggagtatc agcgtcagcg cgatccgcta catcagtttg
    10441 ccagccagca aaccccggaa gcgcagcttc aggcattgca ggataaaatc cgcgctaatc
    10501 cgcaaaacag cgaacagtgg gcgttactgg gcgagtacta tctgtggcaa aacgattaca
    10561 gcaattcgct gctggcgtac cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt
    10621 atgcggcgct ggcgacggtg ctttattacc aggccagcca gcatatgacc gcccagactc
    10681 gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga aatcaccgct ctgatgctgc
    10741 tggcttccga tgcgtttatg caggcgaatt acgcgcaagc catcgaacta tggcaaaaag
    10801 tgatggatct caactcaccc cggatcaacc ggacacagct ggtcgagtcg attaatatgg
    10861 cgaaattgtt gcagcggaga tcggattaat catctggtga tgaagattat gttattgagc
    10921 tgcataaaaa ataatcgaat gaacatatac caaaaataat cactaatcag tattattgca
    10981 gattaaacaa ataaaaatct ttccataaca aatggttatt cattaatcct gccataaact
    11041 cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc
    11101 gcgcaaatga tactgatcat aagcgttaaa aaaatctaca aaccaacgca acacaattca
    11161 tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg gaacggcgct ccattgagga
    11221 agttattctg atgaaaaata taaaattcag cctggcctgg cagattctgt ttgctatggt
    11281 gctgggcatt ctcctgggaa gctacctgca ctaccatagc gacagccgcg actggctggt
    11341 cgtcaatttg ctctctccgg cgggtgatat cttcatccat ctgatcaaaa tgattgttgt
    11401 gccgattgtg atctccacgc tggtggtggg tatcgcgggt gttggtgatg ccaaacagct
    11461 cgggcgtatt ggcgcgaaaa ccattatcta cttcgaggtg atcaccaccg tcgccatcat
    11521 tttggggatc actctggcga acgtcttcca gcccggtgcc ggggtggata tgtcgcagtt
    11581 ggcgaccgtc gatatctcga aatatcagag cactacggaa gcggtacaaa gcagttccca
    11641 cggcattatg ggcacgattt tgtcgctggt gccgacgaat attgtggcgt cgatggcgaa
    11701 aggcgaaatg ctgccgatca tcttcttctc ggtgctgttt ggtctggggc tttcctccct
    11761 gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc tccatctctg aaaccatgtt
    11821 taaagtgact cacatggtga tgcgttatgc gccggtgggt gtgtttgcgc tgattgcggt
    11881 gacggtggct aactttggtt tctcttctct gtggccactg gcgaaactgg tgctgctggt
    11941 gcatttcgcc attctgttct tcgcgctggt agtgctggga attgtggcgc gcctgtgcgg
    12001 gttaagcgtc tggatcctga ttcgtattct gaaagatgag ctgattctgg cgtactccac
    12061 tgccagctct gaaagcgtac tgccgcgaat tattgagaag atggaagcct acggagcacc
    12121 ggcgtcgatc accagcttcg tggtgccaac cggttactct tttaaccttg atggttcgac
    12181 gctgtaccaa agtattgccg ctattttcat cgctcagctg tatggcattg acctgtccat
    12241 ctggcaggaa atcattctgg tgctgacgct gatggtgacc tcgaaaggga ttgctggcgt
    12301 gccgggcgtg tcgtttgtgg tgttgctggc aacgctgggt agcgtaggta ttccgctgga
    12361 aggtctggcg tttattgctg gtgttgaccg tatcctcgac atggcgcgta ctgcgctgaa
    12421 cgtggtgggt aatgcgctgg cggtgctggt gattgccaag tgggaacaca aatttgaccg
    12481 caaaaaggcg ctggcttatg agcgtgaagt gctgggcaaa tttgataaaa ctgcggatca
    12541 ataattgaag attgccgggg ttatcccccg gcaatgtgtg gatgcctgat gcgacgctgg
    12601 tgcgtcttat caggcctacg tcagacagcg caatagcctg atttagcgtg attttgtagg
    12661 tcggataagg cgtttacgcc gcatccgaca tcaacgcctg atgcgacgct taacgcgtct
    12721 tatcaggcct accttaaccg ttggttaatt tttcaaactc ttcacacccg gtatcaaacc
    12781 cttccataca gctcaggttc aaccagtgca gcgccttttg tttattcttc tcgataaaac
    12841 cttcttcacc gtttaagaac atcattcccg cccagtactc ggaataaccg gttcgggaaa
    12901 ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc gtcggcctta atgcccacgc
    12961 cgttggcgta aatcaaacca agcagcattt gggcatcgac ggcagagtcg ttctccagat
    13021 cttccgaggc gttttctaac agcgaaatcg cctttggata atccggttta cccgcttgag
    13081 tatttaccag aatatgcgcc agcgttactt caccttcttt actgcccgct tgcgccgctt
    13141 tctctgcaag aacttttgct tgtggatagt ccagactgac cggattggtg attttgatct
    13201 gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc ggctttctgt gcccaatatt
    13261 cggctttgct caaatcgccg gagctaaacc agctgtcggc aagaaaatat tgtgcgcgtc
    13321 ggtccccggc ctctgctgcc tttaaatact ggctgccagg ctcggagtcg ttggcgtggg
    13381 caaagaatgt caaaaacaac atcaatgcga taattttttt cattttgata atacgattgg
    13441 gtagggagta accagtataa agagttcgaa tggataaaaa aacagcctcc gaaaggaggc
    13501 tgtagaaagg actgtattac gccagtgccg cttcgcgcag gcgagttttc aacttgttgt
    13561 actcgtcaat cacgtactgc tcggcggcgc gctgatcggc gatcggctcg acgcgcacgg
    13621 cgcagtattt gtattccggc gttttcgtaa tcgggcttaa gttttcggta accagctcgt
    13681 tacaggcacc aatccaccac tggtaggtca tgtaaatagc ccctttgttc ggacgatcgc
    13741 tgacctgcgc acgggtgata attttgcctt tacgcgagtg cacccaaacc aatgcctcat
    13801 cttcaatacc cagacgtttg gcgtcttcgg tattgatttg tgcgtagcca ggttcatcag
    13861 ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg gcaagagtag tgaccaactt
    13921 cacgcaccgt tgacagtacc atcgggtact cgtcggtgag tttgtcgatt ggcgctaccc
    13981 agtcgcaggt gaagaactgc gccagaccgt tcggggtatc aaacttctct ttaaacagat
    14041 aagaagtccc ctgatcggca tctgaagtat cgcggcaagg ccactgaatg aagcccagtt
    14101 cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca cagatgacgc aactcatccc
    14161 agatctcctg ggtgttgttg tagtgcatcg gataacccat acgggtggcg atttcactga
    14221 tgatttgcca gtccgttttc agatcccatt tcggttcaac tgctttgaag aaacgctgga
    14281 agccacggtc agccgcagta aacacgcctt catgctcgcc ccacgacgtt gacggtaaaa
    14341 taacatccgc cgccgacgcg gttttggtca taaagatgtc ctgaacgatg accagttcca
    14401 gatcttcaaa ggctttacgt actgccgaca gctccgcgtc agtttgtagc ggatcttcgc
    14461 ccataatgta cgcggcacga acttcgccat gcgctgcgcg gtgcggcagc tcgctgatgc
    14521 gataaccggt atgagcaggc aggctttcca cgccccaggc tttggcgaat ttctcgcggt
    14581 tagccggatc tttcacgtac tggtatcccg gatacgtatc cggcagcgcg cccatatcgc
    14641 aggcaccctg cacgttgttc tgaccacgaa ccgggtttac acccgcatgc ggcttaccga
    14701 ggttaccggt cagcatcgcg aggctggtca gagaacgcac ggtttccacg ccctggtaga
    14761 actgggttac acccatgccc cacaggatgg cggcgctttt cgcctgggca tacatccgtg
    14821 ccgcctgacg aatctcactg gcgctgacgc cggtgatatc ttcaaccgac tccggcgtgt
    14881 agccttcaac gattttacga tactcttcaa agccttctgt acgtgaagcg acgaacgctt
    14941 tgtcgtacag attttcttca ataatgacat ggcccatcgc attcaacagc gcgatgttcg
    15001 agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc ggtttcaatt ttgcgcggat
    15061 cgcagacgat aattttcgcc ccgttacgtt tagcgttaat tacgtgattc gctacgattg
    15121 ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa atcggtatta tcaatttcgt
    15181 taatagcatt gctcattgcg ccattaccga ccgattggtg cagacctgca accgatgggc
    15241 cgtgtcagac acgagcgcag cagtcaacgt tattggtacc aataacggcg cgcgcaaatt
    15301 tttgcattac atagttggtt tcgttacccg taccacgcga ggagccggtc gtctggatgg
    15361 catccggacc gtacttctct ttgatggcgc tcaggcgctc ggcaacgtaa ttcagtgcct
    15421 catcccagga aacaggttcg agtttgccgc cacgctggcg acggatcatg ggggttttca
    15481 ggcgcggggt caggatctgg gtatcgttaa tgaaatccca gccataataa cccttcagac
    15541 acagggtacc ctggttggtt ttcccctgcg ctgcctccgc ccggacgatt ttgccgttat
    15601 cgacgaccag gttgattttg caacctgatg cgcaataggg gcaaaccgtg acgacttttt
    15661 tcatcggtct cgctccagtt aatcaaatca cgcatacgcg ctctcgacta cagtatgcat
    15721 cttttatgcc acattttatt tggggtcatt ccctgatatt acgggcacta tttattcaaa
    15781 actctgacga aaaacaggct gtcgtcagtt ttgacgtgac gaaacgaaat accgcgtgac
    15841 agccatcacg cggcagacat tttatttttt ctcgacgacg ggacctgcct gatacccgcc
    15901 gcccagcgat ttcatcaact gaatgctttg gatcacccgg cggctgtcca gcatcagtaa
    15961 tgacatctct tcggcaagta ctggcaaccg ggcttcagtg gcctgtaagc ggctggttaa
    16021 gccgcgctga taggcggcct cggcagcgcg ctgggtgaag cgcgttgctt ccacgcgttc
    16081 agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc gtgccgttga cggcaacgtc
    16141 acgcaccgcg ttcagtactg actggttgta acgttcaatc atcatgttgc tggcggcgcg
    16201 agtgccttcg agattggcat tcaaccgtcc accgtcaaac agcggcaatt tcagacccgg
    16261 gatgaagttg aactggcgac tggttttttt gaataaggta tccaggtgga tggcatcaag
    16321 gccgaaaaac gctttgatat caaagcttgg gtagaacagc gcccgcgcgg aatccacctg
    16381 atctaatgac gcctgaacat accagcgcat ggcttgcaga tccgggcgtc tggcgagcaa
    16441 ctcataagag agcgtcgccg gaatgccagt ctggacttgc ggtaatgcca ccggtttgat
    16501 ctccggcata tcgctggctc ccgcgccaat caatgcgcgc agagattctc gcgtttcggt
    16561 gatttgccct ttaacggcag caatttgttt atcgaccgcc agaatctgtg cccgcgcgcc
    16621 gtggaaaggc acttgcgctt ccagaccgtg tgccacttta ctttggtgcg ctttcaccgc
    16681 gtaatcaatc acatcgcgag tttgttctaa cagatcgagc atctgatagc tggcctgcat
    16741 actgtaataa agctgcgcta cgcccgtggt cagcgatagc tctactgctg cggtttctgc
    16801 cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct gagcgatgca caccccacaa
    16861 atcaagatcc agtccggcaa acaaacctac tgtggcttcc gtatagtacg gcccgtccat
    16921 acccagtgct ggcgcatcca tcgcataagg gcttaaaaag ccgttcgccg agacgcgttg
    16981 gcggttgagc atccctaacg ctgcgacctg taattgtgaa ccggcatcta acaaatcggc
    17041 ctgggactgc gctttttctt cccgcagttt cgcttcggcg agggtgtgtg aaccacttag
    17101 cgtccgttgg atcagggcat ccaactgcgg gtcattgagt tgtttccacc actgcgcctg
    17161 cggccagccg gagctggcaa gatgaatatc atcggccagt ttgatttgtt ccggtttgag
    17221 ctgttgatga ggcgcagaat ccttgcgtac cagggcacag ccggaaatca gcgtcgtgct
    17281 gccgagaatg ctgcacaaca gcagacgtga aagttgacga ttgatcattg cgtggctcct
    17341 tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc agtctggcaa gcgggcgaaa
    17401 agctggcaga cgtgttgctc ctgttttaat aaggtgggca ggatggcctg cgaggcgggt
    17461 gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg ttgccagacc ggcagcgtat
    17521 ttttccagag cgtcggcaaa ctgacctgct ctgtccggct gtagtgcgtt atccagcgcc
    17581 tgcgccgagt tccaggtggc atcccaggcg tgaagaagat cgcggccctg acgaataacc
    17641 gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt ccagttgacg ctccagcgcc
    17701 acgcgttggc acatctcttc acaggcatta aacgccgcat gcagacctat acgaatttgc
    17761 agataagtgc gcagagccgt gacttcctgc tggcgtggga tccgcattac tttacttaac
    17821 atacccagcg cgacagccag tttttgcggc agcgtgcgcg ctttactttc aggccagaca
    17881 aaggtgtaaa tcaccgcgga caccacggta ccaatgatga tacccagggc gcgatcgcga
    17941 atttccacca ggtcgtacac cgggccaaaa acgttttcga gcgtggcgag cgcgaaggta
    18001 accaccatct gtgtgccgat ataagaagag cgttcagagc tggtggcaat ccatgcgccc
    18061 aacaggaaaa tcggtgccag cacaaacagc aattcgacaa tattgtccag ccagggcatg
    18121 accagtagcg tgaataacag cgccagaatc gcgccgcaaa aggccccgcc aaaacgcagc
    18181 accatcttct ggtacgatga accgacattc ggattagcga cgatcacgca ggtcagcata
    18241 caggtgtgaa tgccttccca gtccacgccg ctgtagaagg tgtaacagat caaacaggcg
    18301 agcagcgttt ttaccgcgta gcgcatatag tctggattgg taaaagcatc ggcggccatt
    18361 gatggcggtt tggctgcggg cgttggcggc gtattcgggt ccatctgacc aagctgtaac
    18421 agtgtctggc agatattctc caggttacat tcccgtgccg ccatcgcttc actttcactg
    18481 atccgccagt cgctttgcca gcactgacct tcggtaatgg catgctgcag cttgttgatt
    18541 tctgaagcta atttttgtcg gaattcaata attgcctgag aatcagcaaa agaggtggga
    18601 tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg ccacgcagct ttgccaccat
    18661 gcactctggg ttcgccagtt ggcatcgtcc gcgaggcaaa agacattgag tttttgcagc
    18721 gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg cgaggctgtc cgtcaggtgg
    18781 ctaatggcat catcaagccg atcattaagc acctgatgca tttgcgaaat ggcacgactg
    18841 ggaaaccaca gcacgccgat taacgtcatc agcaaggttg gatagaggcc aacaacgata
    18901 caccacagcg ttaagcgcac gaccacttcc ggatagtcga gcatggcggg gaaggtttgc
    18961 ccgtaaatag cgacaatggc gacggcgaaa aagaccagcc ccaagcgatg ggtgcgcatc
    19021 aaaaacatgc agcccatcag gatcggtccg gcgatgatca agcggatcaa cggttcgccg
    19081 tatgaccatt tgtagatcaa aaacaggctg ccgatctcca gcaccgtggc aaccacaaac
    19141 aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa taccgtaaaa cagcactgcc
    19201 agcgataacg ccacaaaagg gatctcaaaa gtcatcgaga tcagaatcac cagcaggcag
    19261 cctacccaga gttgcacggt ctgcggcacg cgacctggtc gccgctcgct taactcttca
    19321 tgaaagaacg ccagcagcct gaccaccggt aatggcaggg agttgagcgc gctcattatt
    19381 gtggctcaag gttagcgact gccgaagcgc cgatgcggaa catttccggg tcaggtttat
    19441 caaccatgat tttgaccgga aaacgctggg caacgcggac ccagttaata gaacgtgata
    19501 ctttcggcag accgcccagc accaggccgc cgtcatccgg cagtacgccg tagccaatag
    19561 aatccacttt accttcaaag gttttgccgc tgtcgctcat caggcgaatg gttgcaggcg
    19621 taccagcatg aatgtttttc aaatcggttt cgcggaagtt agcgataacg taccagtgac
    19681 gagtgtcgat tagggtaaaa atagggcgca tggcagaagc aaattgcccg acggaggttt
    19741 tgagggaaat gacccggcca tcaaacggcg cgcgaacggt cgccatttcc agatgcagtt
    19801 tggtcagggc aatatccgct tcgaccgccg cacgttgcgc caccagtgca tcgacgccgc
    19861 tgacggcgct ggcggctgac tgtgcctgta acaatacggc attaagatcg gcttctgcag
    19921 cgcgctgcgc ggtcctcgca cggtcaacgt cttccgctga gacaaaacct tctttcagta
    19981 atggctcggt gcggcgtaat gtatctgtgg cctgtttagc ggcggcacgg gctttttcta
    20041 ccgtggcgtt aaccgagtcg gcaccaaact gttgcgcgtc aacgctacgc tgggtgagca
    20101 taatttgctt atccagtgcc gcgagggagg cttcagcttt cgccagattg gcttcgtacg
    20161 ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac tgcctggttg tcggtgaccg
    20221 ccagttctac aatgcggccg ctgacttccg gcaccacatc aatggtatct gctgacgcgt
    20281 aagcgtcatt agttgatggc gcactgtcta cgcgccagat aacgaaaaca agggcaacca
    20341 gcgccaacgc aaccactaac agagcaggga atttactgcg aggagctttt ttcggcgtac
    20401 tttccatgag cgcgtctcaa atagattaga aaaatgccag ccagaacagc atggcgtaga
    20461 gagcaaaaag ggcggtataa ataattccga caaatgccag attgatattt gcccgctgaa
    20521 taattcgcct cgtgatgagc gtcaaaataa ggctggcaat ggcgcagaaa aaccagctgg
    20581 gataataagc gccgatcacc gggattgccg gagataatga gcatcccgaa accatgaaga
    20641 cgggaattat ccaggctgtt tttttgaggt gcatcgccat gcgagagaga actgttggca
    20701 tattaaatac atcctgtctg ctgcgaacaa tatctgtagt gaatatcaat atccattgaa
    20761 ggcgtcagta tggctatttt cttgatgaat aaaaatagct ttattcagcc accgcattca
    20821 taacttatat attttatgta ttaatttgct gcgtatattt tttaaataaa tgtttttggg
    20881 ggcctaagtg cccccaaaag cgagtgacat taatttgggg ttacgatatt cacccacaaa
    20941 tcgaaattat ccaggcaggc aataatttct tccagtttcg caccattgcc aataattttg
    21001 gctttcttcg cttttaccag atccgccatt ttggcttgtc cggtcagcac ggcgtgcaga
    21061 tcttcacgac tgatgtagaa agaggcgtca gcttgcggtt gcagcgtttt acggtagtta
    21121 agcacgctat cgttcagcgt caggttgagg ttatcgccgt tgctcatatt gaagttcagg
    21181 ctgatatttt tacccgcagc tttcgcgcta tcgaggcgaa cggccataaa gtcgaacagc
    21241 atttcgaccg acattccgcg aatggtgtcc ggggaaccgg tggtgccgtg gctgaactta
    21301 tgcacccctt cgcgcagctc tttcgcgccg gtcaggtaga aaccgcgcca tgtggcggac
    21361 tcggcctgat agcccagctg ttcaaagtta ttcgcttgca gattcttcgc gacctggtca
    21421 cccgggttgg cggcaatcac ctgtttcagc agttctgccg accagcggta atcaccttgc
    21481 ttgttcgctt cttgcgccag gttgatgaca cgggcagaac cgcccagcgc tttcacgtaa
    21541 cgtttgccca tctctacctg accatacgga tgcaggttag ccgggttacc gtcgtaatag
    21601 ccaagataga agttatacac cgcgcgggcg ttgtggctga cagaaccgta atagccacgg
    21661 ctggcccagt tattggcaag tgcaggcggc agcttaatca tgtcgccgat ttcattcatg
    21721 gtgtagccct ggttcgccag gtgcagggtc tggtcgtgaa tgtacttgat agtatcgcga
    21781 tatttaccaa tataatcatt gatatgctta ttgccccaga ccggccatgt gtgcggcata
    21841 aacagtactt ccgcgtcgtt accccacata tccagcgttt cgttcaggta ctcggtccac
    21901 ttgctggtat cgcgggtttt cgcgccgcgc agagtgtaga agttgtgcag ggtatgcgtg
    21961 gcgttctcgg cggtacacag ggctttcagg gccggaatat agaagtgcat ttcggctggc
    22021 gcttcgctac ctggggtcat caggaagtca aactccaggc cgtcgataat catcttctcg
    22081 ccagttctga cgatagtttt agtcggtgca ataatgctcg ggtcgcccgt tgccagcgtc
    22141 acgccaaggc cattacccac gttgccttgc gcgttgtgcg gcagtaacag accgtaagag
    22201 tagagcgcac ggcggctcat gatgttaccc gccagcacgt tttcgctgat ggcttcgtcc
    22261 ataaagccag caggggcaat cacctgaact ttgccggatt taacatcggc ttcagagata
    22321 atgcctttca cgccaccata gtggtcggtg tggctgtgag tgtagataac ggcaacaatc
    22381 ggtttttgcg gacgatgctg gaagtaaagg tcaagtgcgg cttttgcggc aggcggcgtc
    22441 accagcgggt cgataacaat aatgcctttc tcgccctcaa cgaacgtaat gttagagata
    22501 tcctggccgc gcacctgata cattttatcg gtgactttga acaggccaga aatgccgttg
    22561 atctgcgact gacgccacag gctggggttt atggtttccg gcgctgcggc attaatatca
    22621 aatttgtagt cgtccgcgcg gtagtaaatt ttaccgttcg catcacgcag aataccttca
    22681 tccagcagcg gggcgataaa tccacgctgg gcatcgtcaa aatcctgacg atcgctaaac
    22741 ggtaatgatt tggcgtaatt ctgattaacc tgttgggtat attgagtggc ggctttcgct
    22801 tcctctttcg cgttagcggt atttaccatc atgccgctgg cggcagttaa cgcaataaca
    22861 atcctgctca aacggaataa ccgagagtta ttcatataaa tctccattta tcatttatgt
    22921 cgtaaatatg taatccatgc aaaatacgtc atttaaagct ggcgttatca taattgggaa
    22981 attgcgactt taataagtgg aagtgtgagc ggaacgatcc attttattag gcatttgtgc
    23041 tgaagatgga aa
//
LOCUS       DAZFFG010000077        22756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFFG010000077 DAZFFG010000000
VERSION     DAZFFG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.077"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            12..1034
                     /locus_tag="ACHJ7J_003255"
     CDS             12..1034
                     /locus_tag="ACHJ7J_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO5001446.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQAYLASRFVGEAQAFATSPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1034..2014
                     /locus_tag="ACHJ7J_003256"
     CDS             1034..2014
                     /locus_tag="ACHJ7J_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5001447.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2011..2769
                     /locus_tag="ACHJ7J_003257"
     CDS             2011..2769
                     /locus_tag="ACHJ7J_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5001448.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2779..3591
                     /locus_tag="ACHJ7J_003258"
     CDS             2779..3591
                     /locus_tag="ACHJ7J_003258"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5001449.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLISKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            3588..4442
                     /gene="modD"
                     /locus_tag="ACHJ7J_003259"
     CDS             3588..4442
                     /gene="modD"
                     /locus_tag="ACHJ7J_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO5001450.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAIDQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4468..6438
                     /locus_tag="ACHJ7J_003260"
     CDS             4468..6438
                     /locus_tag="ACHJ7J_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5001451.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDDSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(6488..6742)
                     /locus_tag="ACHJ7J_003261"
     CDS             complement(6488..6742)
                     /locus_tag="ACHJ7J_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5001452.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6943..7677
                     /locus_tag="ACHJ7J_003262"
     CDS             6943..7677
                     /locus_tag="ACHJ7J_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO5001453.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIS
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7679..8290)
                     /gene="emtA"
                     /locus_tag="ACHJ7J_003263"
     CDS             complement(7679..8290)
                     /gene="emtA"
                     /locus_tag="ACHJ7J_003263"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO5001454.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8390..9304
                     /gene="ldcA"
                     /locus_tag="ACHJ7J_003264"
     CDS             8390..9304
                     /gene="ldcA"
                     /locus_tag="ACHJ7J_003264"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO5001455.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWPALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLASVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAMLNNTREGTQLTISGHPVLKM"
     gene            9399..11135
                     /locus_tag="ACHJ7J_003265"
     CDS             9399..11135
                     /locus_tag="ACHJ7J_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO5001456.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11238..11390)
                     /locus_tag="ACHJ7J_003266"
     CDS             complement(11238..11390)
                     /locus_tag="ACHJ7J_003266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001457.1"
                     /translation="MLGGDIDNMGLVFLHLLQSEEAITLTFLSRCFELIGQSLQWFVI
                     HNLTFF"
     gene            11756..13198
                     /locus_tag="ACHJ7J_003267"
     CDS             11756..13198
                     /locus_tag="ACHJ7J_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001458.1"
                     /translation="MASFKDICPFAPAIPPSSVNTGVRRPPGRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIVDLSIAGKVNIQVDNNTIITLGTPLNQQHNEFMMVAKWCEW
                     AIQQDGLQENLQKKLHEVLEENRQNKQSEIPQEDLKERLKEIKEDILKENQSASQIED
                     RAEALRRMKECLITRQSMLDLSNLGLTSLPENLPPHLIEFNCSRNVLTALPEVMPKGL
                     RVLECMENFLILLPKVQPPKLMVLKCYENYIIWLPELSTNLRVIDCSENFLQFLPPSM
                     PQYLYTLRCAFNSISLIPDEMLENLTRLKVFDCSCNDLISSPRLPPKLIIYYCGENQF
                     KTVLVPQPQSLKVFDCNGNPWDKDNLPTLLKAVEGLKNQQGLEELLDFLDKEGLVNLK
                     KLEKLEYQEELDLEALEHLEDPVLLERVKVQEDLELLNQQLGLLSLERQQDSQPVNQH
                     SEHEPESASRVKRDLSEVEFESTMKRKRFM"
     gene            complement(13367..14437)
                     /gene="dadX"
                     /locus_tag="ACHJ7J_003268"
     CDS             complement(13367..14437)
                     /gene="dadX"
                     /locus_tag="ACHJ7J_003268"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO5001459.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQASEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14447..15745)
                     /gene="dadA"
                     /locus_tag="ACHJ7J_003269"
     CDS             complement(14447..15745)
                     /gene="dadA"
                     /locus_tag="ACHJ7J_003269"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO5001460.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVNIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16075..17607
                     /locus_tag="ACHJ7J_003270"
     CDS             16075..17607
                     /locus_tag="ACHJ7J_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO5001461.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKFYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(17659..18378)
                     /gene="fadR"
                     /locus_tag="ACHJ7J_003271"
     CDS             complement(17659..18378)
                     /gene="fadR"
                     /locus_tag="ACHJ7J_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO5001462.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18600..20135
                     /gene="nhaB"
                     /locus_tag="ACHJ7J_003272"
     CDS             18600..20135
                     /gene="nhaB"
                     /locus_tag="ACHJ7J_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO5001463.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLILQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMGWI
                     ATL"
     gene            20281..20811
                     /gene="dsbB"
                     /locus_tag="ACHJ7J_003273"
     CDS             20281..20811
                     /gene="dsbB"
                     /locus_tag="ACHJ7J_003273"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO5001464.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKLDLFGR"
     gene            complement(20857..22125)
                     /gene="umuC"
                     /locus_tag="ACHJ7J_003274"
     CDS             complement(20857..22125)
                     /gene="umuC"
                     /locus_tag="ACHJ7J_003274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO5001465.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFTLNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22125..22544)
                     /locus_tag="ACHJ7J_003275"
     CDS             complement(22125..22544)
                     /locus_tag="ACHJ7J_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5001466.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5425 a         5972 c         5738 g         5621 t
ORIGIN      
        1 ggatataact tatgtcacta accagacgga ggtttacaca gattcttgcg tcgacgttgt
       61 tcctgcatca tctgccttct tttgcacaat cagtcaaatt ctgggcctca ctgacgcttc
      121 ccgaagccca aaacattaca cgcatcgtca gcgcaggtgc gcccgccgat ttgctattgc
      181 tggctgtcgc gccagaaaaa atggtcggtt tttcctcttt tgattttgcc cgtcaggcat
      241 taattccatt gccagagcac attcgccagt tacccaggct gggacgactc gccgggcgcg
      301 ccagcacact ctcgctggaa gggcttatgg cgttacatcc cgatttggtc gttgattgcg
      361 gcaatacgga tgaaacctgg atctcccagg cacggcaggt tagcgaacag acacaaatac
      421 cctggttatt gcttaacggg aaactggcgc aatcagcaga acaattaaca acgcttggca
      481 aaacgttagg cgaagagcat cgcgccgcag aacaagccta tctcgccagc cgcttcgttg
      541 gtgaagctca ggcattcgcc acctcacccg ccgctaacct ccgcttttat gctgcgcgcg
      601 gtcctcgagg gctggaaacg ggcttaaagg gttcgttgca taccgaggcg gcggaattat
      661 taggtttgca caacgtcgcg caaatagccg atcgccacgg cctgacacag gtttccatgg
      721 aaaatctcct gcgctggcag ccggatatta ttctggttca ggaggccgtt actgcagact
      781 ttattcgtcg tgatcctctc tggcagggcg tgaaagccgt tgcggaacaa cgcatcttat
      841 ttttaagtgg cttgcccttt ggctggctgg atgccccacc gggaatcaac cgtcttctgg
      901 gattacgcag acttcacgcc tggctggatc ccgccataaa tcgccagttt aaaagtgaca
      961 tgcagcatta cgcccaactg ttctggcatt gttcactcag tgacgccgac tatcaaaaat
     1021 tggtggcgag ctaatgagaa tcgtaaacgg atgtattttg ctgctggcag ctatcagtat
     1081 cacttttgcc gccgtaagtg gggcttatca tctcgatatg cagcaactgc tggcgctaat
     1141 tcttcgtcaa gaaaatgttc ctgtgcagga acaaattgtc ttctggcaaa tccgcgtgcc
     1201 gcgtattctt gctgcgctgt tactgggcgc ggcgctggca ggcgccggaa ccacctatca
     1261 gggaatgttg cgtaatccct tagtttcacc ggatatttta ggcgtctccg ctggtgccgg
     1321 acttggtgcc tgcacggcaa ttctgtgggg attatccatt gtttacatcc agctgtacgc
     1381 attttgcggc gggctgatgg tggtcgcggg cgtctggttg attacgcgtc gggtgacccg
     1441 ccacgatccg atcttaaccc tggtgctggt aggcattgcg ttaggaacgt tgtgcggagc
     1501 ggggatatca ctaattaaaa cgctggccga tccttacacc cagttaccgt caattacctt
     1561 ctggttgctg gggggactgt ccaccgtgac cttacgcgat ctctgctatg ctgcaccgat
     1621 cattctcata ggctccttgc ccctgttttt tctccgctgg cgcatgaatt tactcacgct
     1681 ttccgacgac gaagcccgtt cgctgggact gaacgtgacg cgtctgcgct ttggattgat
     1741 tgtctgcgcc acactaatca ccgccagtac ggtagcaata gcgggcataa ttggttgggt
     1801 cggcctggtc gttccgcaca ttgcccgctt gttgaccggt cataaccatc agcagctgtt
     1861 gcccatggca atgtgtactg gcgcgattct gctcctgctt accgatacgc tggcacgcag
     1921 cattggaaca acagaaatcc cacttggcat tctgactgct tttgttggcg caccgttttt
     1981 cctgcttttg ttgttacgcg gaggtcgcca gtgactttgc ttgccgtccg tcatgcgtct
     2041 ctgggttact cccgccatcc ggtgctacgc gacgtgtcct tcaccctgtc gcaaggaaca
     2101 atttgttgtc tgttaggagc caatggttgc ggcaaaacca cactcatgcg ctccattctt
     2161 ggcgttatac cgttattaaa aggcgaggtc ttactggata gcctccctgt gcagacactc
     2221 agccatcgcc agcgcgcgca ggctattgcc tgggtgccgc aggcgcacga cggtattttt
     2281 gcttttagtg tgctggatat ggtgttgatg ggcctggccc ccaccatagg cgctttttcc
     2341 gttcccggta agcaagaacg cctgaaagcc acggagcaac tggaaaagtt gggcattttg
     2401 catctggcgg cacgacgctg gaatacgctt agcggcggtg aacgtcagct ggctttaatc
     2461 gcccgtgcac tcgtccagca accgcgcttg ttgttactgg atgagcctgc ctccagcctc
     2521 gatttcggtc atcagatcca actactggac acgctggcac agctcaaaaa taacggcatg
     2581 acaatgttaa tgtcgactca tcatccgctc catgccaacg ccattgccga cagcattatc
     2641 caggtagagc cagatggacg agtaacacaa ggcttaccga ccgaacagtt aacaacaaac
     2701 aaactggctg ccctgtatcg ggtttctgcc gaccagattc atcatcacct ctctgcaatt
     2761 agccactaag gaacaatcat gctcattgat gatattgatt ttgcagacct ctacctgcag
     2821 caactcaaac tggcccaccg cacagaaaaa acgccggatc actgggatca acgggcggaa
     2881 aaaatggcgg aaaattgcgc ctcgcctacg gacagctacc tgcaacaact gatctcaaaa
     2941 attgacctac aaggcgctca gaccttattc gatatgggct gcggaccggg taccgtatcg
     3001 ctggcgctgg ctgacaaact cacgacgatt tatggcgttg attacagtca ggggatgctg
     3061 aacgttgccg cccgtcgcgc cgccgcgctg aaggcagaca atgtccactg gatccagcgc
     3121 gcctgggaag aggactggag tgacctgcca cgctgcgata ttgccgtggc ttcccgctcc
     3181 actctggtag ctgatatgcg ccaggcaatg agcaaactga ataatcaggc acggcttcgc
     3241 gtctatacga ctcatctggt cagcacctcg ttcgtctctc cagccattca gcgggcggct
     3301 ggtcgggaag tgatcgaatt acccaactat atctttgcgc tcaacgtcct gtatcagatg
     3361 ggaatttatg cccacgttga ttttatccgt ggacaaaatt gccaacagga taacagcacc
     3421 tgggaacgtt ttgagcagaa cgtcagctgg agtcttggcg cgcttaacga cgatgagcgt
     3481 gagcggttat atcgctggta tcaacaacaa gatgctcgtg ccctcgcccc tgccagccgc
     3541 gactgggcgc tcatctggtg ggatagcgta ccgcaggagg cactgcgatg attttccttt
     3601 ctcaggcaca aatcgatgcg ttacttctgg aagatatcca gggcggtgac ctgaccacga
     3661 gagcattaaa tattggacac cagcatggct atatagagtt ttttctccgt cagggcggct
     3721 gcgtcagcgg cgtttctgtc gcgtgtaaga tgttaactac gctgggatta actattgatg
     3781 acgcggtcag cgacggttca caagcgaacg cgggtcagcg gctaatccgg gcgcagggta
     3841 atgccgcagc acttcatcag ggatggaagg caatccagaa tgtgctggag tggagttgcg
     3901 gtgtttctga ttatctcgat caaatgctgg cgttacttcg tgaacgttat cctgatggca
     3961 atatcgcctg cacccgaaaa gcaattccgg gcacccgctt actggcctcg caggcaattc
     4021 tggctgccgg aggactgatt catcgtgccg gatgtgcgga aaccatatta ttgtttgcca
     4081 accaccgcca ttttcttcat gacaatcagg actggtcagg cgcaatcgat cagttacgtc
     4141 gccacgcacc cgagaagaaa attgttgttg aagccgacac gccgaaagag gcaatcgccg
     4201 cgttacgcgc gcaaccagac gtacttcagc tcgacaaatt tagtccgcaa caggcaactg
     4261 aaattgcgca aatagcgccg tcgctggctc cccactgcac gctggctctg accggcggaa
     4321 ttaatctgac gacactcaaa aattacctcg actgcggcat tcgcctcttt attacctccg
     4381 cgccttatta cgcggcaccg gctgacatta aagtcagtct gcaacccgca gcctctattt
     4441 aattctttgt taaacggaat ttattctatg agactcaaaa aacattatct ctgcacagca
     4501 ttatcacttg cctttaccca gcaggccgta gcggctcagg agagcgactc gctgaccgta
     4561 tggtccagtc cggtatcatc gacgacgacc accgttctcg atcaacccac catgaaggcc
     4621 ctggataaac agaatgtcgc tcaggcatta agtgtcgtcc ccggcgtggt gctgcaaaag
     4681 tcaggcagcc gcaacgaaga acaggtaaaa gttcgtggct ttgatagtcg gcaggtgccg
     4741 gtctatttcg acggtgtgcc catttatgtt ccctatgacg gcaacctcga tctggcgcgg
     4801 attctgacca acaatctggg ggcagtcgaa gtttccaaag ggtattcgtc gctgcttcag
     4861 gggcctaatc agatgggcgg agccattaat atcaccaccc agaagccaac aaaacctctg
     4921 gaagcaagtc tgggatatcg ccagggatgg agccgtagcc aggacaatgc ctacgatatg
     4981 catgcttcat ttgccgccag tagcgatctg gggtatttgc aagtcagcgg tagccagcta
     5041 aagcaggatt ttctcggcct gccgcatggt gtaaataatg atattgcagg caaacacggc
     5101 aagatgatta attcatcggc tgatgataaa cgcggcattg tgaagctcgg atttacacca
     5161 cgtgaaaacg atgaatacac actgacttac attaagcagg atggtgaaaa agataacccg
     5221 ccatacagcg gaaatagtgg tcaaaaatca cgctactggc agtggccaga gtatgacaaa
     5281 gaaagttttt attatcaggg aacgacccaa ctaaacgatc gttttaccct gaaaagtcgg
     5341 ctgtatcgcg acacctttga aaatacgctg atgatgtaca actcgctggc tgatttgaaa
     5401 aataaaaaag gcagctacag ccattattcc gattacagcg acggtgccgg attacaactg
     5461 gcagccgatg tgcgtgaaaa cgatctgctg tcgtttgccg ttaactggaa agatgacgta
     5521 caccgggaaa aaggtgcgcc gcacgccgct tacgatcgct atgaagatcg tacctggtcg
     5581 ctcgccagtg aatatcaatg ggctgctgcc gataatgtcg atgtcgtggc cggaatcagc
     5641 tatgactggc gcgatagcgt agaagcgaaa aaacacgaga aagatgacag catcacccat
     5701 tatgacgaca acaatcagtc agcttttaac tggcaagtga tggggaaata ccactttgcc
     5761 aatgaagaca cgctggcgct ttcgtactat gaccgcacac gctttccgac gctgaaagaa
     5821 cgctatacca catccaaacc tgcgtataac cagatagcga ttgttaaccc gcagctcaag
     5881 ccggaacgcg ctcgcggggt ggatttaacc tggaatggtg ccttcacgcg cgactgggga
     5941 tttgaggtca gcgtttacta taaccgggtg agtgatgcca tcctctcgca caatatcgat
     6001 gccgatacca ttcaaaatca gaacagcggc acggtggatt acagtggtct ggatgccggt
     6061 attaagggga aaatcagcaa tatactggat gtaggattga gctacgccct gatccacgct
     6121 gacgccaaac gcaaagacat cggcaagata accgatctgc caacgcagac aatgaccgca
     6181 tggatgactc tcaaaccgtg ggagccgtta agcgtaacgc tgtcggaaga ggcgcgttcc
     6241 tccagctaca gcaacagtga cggttcacaa aaagccgccg gttttgcggt gacccacatt
     6301 cgagccgatt acaccttagg tcatggcttt agcgtcaatg cgtcggtcaa taacctgttt
     6361 gatacccaat atgcctacag tgaagggttt attgaagaag gccgcaattt ctgggcaggt
     6421 gtcgagtata cgttctgatg tgaaagcgga tagcggcatc tgtatcgcta tccgccttat
     6481 gaaaatctta ttctcttcgc aggaggcgga atacgcccag aacgagaata gctcccacca
     6541 ccgccaccag gaagctgtgc aaattaaaac cgctgatgga gccgccaatg ccaaacatgg
     6601 tcgccagcca gccgccgacc accgcaccga ctatcccgag aatacaggtc aggaaaaatc
     6661 caccaccatc acgccccggc atgattagct tggcgataat gccggctatc aggccaaaaa
     6721 taatccaggc aataattccc atgttcaggc cctcgtttcg atgaatcgga cgcaagaaat
     6781 gcttacgtca acgctctaag tataggcaag gacgggatcg atgctgggcg tttgtcggct
     6841 gatttaagaa caacatgtaa aaaatattca cgcatttgta ttaagttttg ttaactgtga
     6901 ccgataaacc aaagacagtt tgtcagtcag gagtttttcc gcgtgagtca ttaccatgag
     6961 cagttcctga aacaaaatcc gttagccgtc ctgggcgtgt tacgcgattt gcacaaagcc
     7021 gcaattcctt tgcgtatcag ctggaatggc gggcagctga tcagcaaaat actggtaata
     7081 accccggata aactggtgct ggatttcggc agtcaagccg aagacaacat cgccgtacta
     7141 aaggcacagc acattaccat taccgccgaa actcagggtg cgaaagtcga gtttactgtt
     7201 gaacaactac aacagagtga atacttgcag cttccggcat ttattagcgt accgcctccc
     7261 accttatggt ttgtacaacg acgccgatat ttccgcatct ccgccccact ccatccgcct
     7321 tatttttgcc agaccaaact ggcggataac agtacgttac gtttccgcct gtatgatttg
     7381 tcgttaggcg gcatgggcgc attactggaa acagcaaagc ctgccgggtt gcacgaaggt
     7441 atgcgcttcg ctcagattga agtcaacatg gggcaatggg gtgtttttca ctttgacgcc
     7501 cagttaatct ccatcagcga gcgcaaagtg attgatggca agaatgaaac catcaccact
     7561 ccccgtctga gcttccgttt tcttaacgtc agcccgacgg tggagcggca attacagcgg
     7621 attattttct ctctcgagcg ggaagcccgg gagaaagcgg acaaagtacg cgactgattt
     7681 acatcgcgtc cagtgcctgc tcaagtttgt agatatagcg cggagcctgc ggcgcaggat
     7741 gatttcgcgc tacgtgatct aggaactcgt cagcatccag atcgttgatt ttgctgattg
     7801 cctttttccg atctgacgag aaagtccgta gcagcgcacc tgccccgtta gcatatgaca
     7861 ccaccagcgc atattgcagt accttcggat cttcaatgcc tgccagcggg ccggtttcca
     7921 gaatattcag gtaagccgcc cccattgaaa tattacgctc cggatttttc agctcgctgg
     7981 tcgtcggctc accactccag cccatacggc gataaacatc acgaccggag gttgaagctt
     8041 ttaactgcat caaaccaatg gcattcgatt tactcaccgc gttcggatta ccacccgatt
     8101 cgatagcgat aatcgccgtg atcaattgtg gatcgacgcc ccaggctgca ccggctttct
     8161 ggcttattgg catccactgc atcgcacgtt gcaccggaac tttcgcgttc cacggcgggt
     8221 tcgtatagtc atgctttgat gaacaacccg ctaataacac aatcaaaaag gcaaaccatc
     8281 tcaatttcac ttcttctatc cttatagcct ggaatcgccg ttgaggtgac aacggcacac
     8341 ctaagcggca tgatatacat ttcgtttctg ttgtcagcaa ggaattgcca tgtctctgtt
     8401 tcacttaatt gccccatcgg gttactgcat taaacagcac gccgcgctgc gtggtatcca
     8461 gcgccttacc gacgcggggc atcaagtcaa taacgtagag gttattgccc gtcgttgtga
     8521 acgttttgcg ggtacggaaa cggagcgtct ggaagatctc aattcccttg ccagactgac
     8581 cacccccaac accatcgtgc tggctgtacg cggcggttac ggtgccagtc gtttactggc
     8641 agatattgac tggccggcac tggtggctcg ccaacaacat gacccgttgc tcatttgcgg
     8701 acatagcgat tttaccgcca ttcagtgcgg tcttctggcg catggcaatg tcatcacctt
     8761 tagcggcccc atgctggtag caaactttgg cgcggatgaa ctgaacacct ttaccgagca
     8821 ccatttctgg ctggcgttac gcaatgaaac cttcaccatt gaatggcaag gggaaggccc
     8881 gacatgtcag accgaaggca cgttgtgggg aggcaatctt gcgatgctga tttcactgat
     8941 cggtacaccg tggatgccaa aaattgagaa cggtattctg gtgctggaag atattaacga
     9001 gcaccctttc cgcgtcgaac gtatgctgtt gcagctctat catgctggaa ttttgccacg
     9061 tcagaaggcg attattctcg gtagctttag cggcagtacg cctaatgatt atgacgcagg
     9121 ttacaacctg gcgtcagtgt acgcgttttt gcgttcccgc ctgtcgattc cactcattac
     9181 cggcctcgat tttggtcatg aacaacgcac ggttacgctg ccgttgggcg cacatgccat
     9241 gcttaataac acccgggaag gcactcagtt gacgattagc ggtcatcctg ttcttaaaat
     9301 gtaaaaaaat cacgcttcag ggctaagtaa aacgctgtct ctgccgctaa tatggtaggg
     9361 tttatataag agacagcgta atcaggagta accgaccgtt ggatgccaca acaataatta
     9421 gccttttcat cttaggatcc atccttgtca ccagcagtat attacttagt tcattttctt
     9481 cccgtcttgg cattcctatt ctggttattt ttctggcgat cggcatgctg gcgggagtcg
     9541 atggcgtcgg cggtatcccg tttgataatt accccttcgc ctacatggta agtaacctgg
     9601 cgctggcgat tattttgctc gacggcggga tgcgcactca ggccagttcc tttcgtgtgg
     9661 cgttaggacc ggcgctgtcg ctggcgacgc tgggcgtact tatcacctct ggtttaaccg
     9721 gcatgatggc ggcgtggctg tttaatcttg atttgattga aggcttatta atcggcgcta
     9781 tcgtcggctc caccgatgct gcagcggtct tttctctgct gggtggtaag gggcttaacg
     9841 aacgtgttgg ctcgacgctg gaaattgaat ccggcagtaa tgatccaatg gcggtctttc
     9901 tgacgattac cctgattgcg atgatccagc agcatgaaag cagtgtcagc tggatgttcg
     9961 tggttgatat tctgcagcaa tttggcctcg ggattgtcat cggtcttggc ggtggttatt
    10021 tactgctgca aatgattaat cgcatcgccc tgcccgccgg tttatatcca ttgctggcat
    10081 taagcggcgg tattttaatt tttgctttaa ccacggcgct ggaaggtagc ggtattctgg
    10141 ctgtttatct gtgcggtttt ctgctgggta atcggccgat tcgcaaccgc tacggcatcc
    10201 tgcaaaattt cgacggcctc gcctggctgg cgcaaatcgc catgttcctg gtgctggggc
    10261 tattggttaa cccaagcgat ctgctgccta ttgccattcc ggcgctcatt ttgtccgcat
    10321 ggatgatttt cttcgcccgt cctctttcgg tatttgccgg attgctaccg ttccgtggtt
    10381 ttaatctgcg tgagcgcgtg tttatcagct gggtgggatt acgcggcgcg gtgccaatca
    10441 tcctggccgt gttcccgatg atggcggggc tggagaatgc gcgactgttt tttaatgtcg
    10501 ccttctttgt ggttctggtt tcactgctct tgcagggaac atcactctcg tgggcggcga
    10561 aaaaagccaa agtggtggtt ccgcccgtcg gacgtccggt gtcacgcgtt ggcctggata
    10621 ttcatccgga aaatccgtgg gagcagtttg tttatcaatt gagtgctgat aaatggtgtg
    10681 taggtgcggc actgcgcgat ttgcatatgc caaaagagac gcgtattgca gccctgtttc
    10741 gtgataacca gttgcttcat cctaccggca gcacccgact gcgcgaaggc gatgtattgt
    10801 gtgtaattgg tcgggaacgc gatctcccgg cgctcggtaa actgttcagc cagtcgccac
    10861 cggtcgcgct ggatcaacgc ttctttggtg acttcattct cgaagccagc gccaaatatg
    10921 cagatgtggc gctgatatat ggcctggaag acgggcgaga atatcgtgat aaacagcaaa
    10981 cgctgggtga aattgtccag cagttgttag gcgcggcacc ggttgtcggt gaccaggtag
    11041 agtttgccgg aatgatctgg acggtggccg agaaagaaga taatgaagtg ttaaagattg
    11101 gcgttcgggt ggctgaagag gaagctgaat cttaaattta atgaagcatc tatcgccggt
    11161 tgcgatgctt tgcttaacgc agcctgagcc tgtatataaa cttgtcattt cctgattttg
    11221 atatgcacat tccaacatca gaagaaggtt aaattatgga tgacaaacca ttgcaggctc
    11281 tggccaatca actcaaaaca ccggctcaag aaagtcagcg taatggcttc ttctgactgc
    11341 aacaggtgaa gaaaaaccag ccccatatta tctatatccc cgccaagcat caggggcccc
    11401 agcctgacat cgcctgaaca tattccccca cgcaacctgc cgcactggcg tagcccacac
    11461 aataggcgtt tcatccgcct gtagcaccgg atgcatgagc gatttcttat agttattcct
    11521 ttgcttaata acgattattt ccgggtcgtt cgctctcgtc ttttctttaa tacgtcagag
    11581 ctacctgggc cgtaatacag catgtaccag acccgacacg gcatttctga ccagggaaaa
    11641 agtcaacgat gagtagttat atcagcgcaa atgacgtcct gtggctcctt tgcggtgacc
    11701 acattcacgt ctccggcgct gccagaggat atttatttca aggatacgta gcattatggc
    11761 tagctttaaa gacatctgcc cattcgcccc cgccattccc ccatcttccg ttaacacagg
    11821 cgtacgacgc ccgccagggc ggctcttcct gtcggtggaa ctcaagaatc aaattttgca
    11881 gaacatgaag tatctcagcc cgcaaaaatt cagctggggt gatgccccct ggcagattgt
    11941 cgatctcagt atcgcgggca aagtgaacat acaggtggat aacaatacca tcatcacgtt
    12001 ggggactccc ctgaatcagc aacataacga gttcatgatg gtcgccaaat ggtgcgaatg
    12061 ggctatacag caggatggtt tgcaagaaaa cctgcaaaaa aaactgcacg aggtcctgga
    12121 agaaaaccgg caaaacaaac agtcagaaat tccccaagaa gacctgaaag aaaggctgaa
    12181 agaaatcaag gaagatatcc tgaaagaaaa ccagtcagca agccagattg aagaccgggc
    12241 agaagcattg cgcagaatga aggaatgtct cataacaaga cagagtatgc tcgatcttag
    12301 caaccttgga ctgacttcac tccctgaaaa tttgcctcca catctgattg aatttaactg
    12361 cagtagaaac gtgttgaccg cgttaccgga ggtaatgcca aaggggctga gagtgcttga
    12421 atgtatggag aactttttga tcttgttacc gaaggtgcag cccccgaaac tgatggtact
    12481 gaagtgctat gaaaactata ttatctggct gcctgagctg tcgactaacc tgagagtgat
    12541 tgactgttct gaaaacttct tgcaattttt accgccatcg atgccccagt acctgtatac
    12601 actgcgctgt gctttcaaca gtattagctt aatacctgat gagatgctgg agaacttgac
    12661 tcgcctgaag gtatttgact gttcttgtaa cgatttgatc tcttcaccac ggctgccgcc
    12721 caaactaatc atatactact gtggagaaaa ccagtttaaa actgtactgg tgccgcagcc
    12781 ccagagcctg aaggtgtttg actgtaatgg taacccttgg gacaaagaca atttaccgac
    12841 gctgctcaaa gccgtcgagg gcctgaaaaa ccagcagggt ctggaggagc ttttggactt
    12901 tttggacaag gagggcctgg tgaacctgaa gaagctggag aagctggagt accaagagga
    12961 actggacctc gaggctctgg agcacctgga ggacccggta ctcctggagc gcgtgaaggt
    13021 acaggaggac ctggagctct tgaatcaaca gttgggcctg ttgagtctgg aaagacaaca
    13081 ggactcgcag cctgttaatc aacattctga acatgaaccc gaatctgcat caagggtgaa
    13141 gcgtgattta tctgaagtcg agttcgagtc aacaatgaaa cgtaagcgtt ttatgtagaa
    13201 cgcctgtgcg gtgagttcag gttccgggga aatagtgtcc atcattttaa tgggcactat
    13261 catgggcaat ggctctgtca cattaacaac tacagttgct gaccagccgg atggatgatt
    13321 ttttttgcaa ccagaagacg ttgcctccga tccggcttac aacaagttac accgtcacaa
    13381 ccgggacacg taacgccagc gcgcacatca actcatagcc caccgttccg gcagcggcgg
    13441 cgacatcatc aattttgatc tccttgcccc acagctcaac cggcgtacca atacccgcct
    13501 gcgggcaagg cgttaaatcg accgccagca tatccatcga aactgtcccc accgtcatag
    13561 tgcgcacgcc gtccactaaa acaggggtac cggtaggcgc gtggcgcgga taaccgtcgg
    13621 cgtatcctgc ggcaacaatg ccgattcgct gctcatcgcg cgcagtataa cgaccgccgt
    13681 agcccacacg ctcgcccgct tttagcgtct ggacaccaat aatctcactg cttagcgtca
    13741 tcaccggacg taatccggta ttggcgatat cacgccactg accggacggc gaagcgccat
    13801 acaaaataat gccaggccga acccagtcaa aatgcgcttc cgggtgccac agagtcgccg
    13861 ccgaattgga caacgaacgc cgacactcga gcccttccga cgcctgctca atacgcgcca
    13921 tcgcgctgga aattccatca ggatgttccg cctcggcaaa atgcgacatc agggtcattt
    13981 cgccaacatt cgccatcgcc cgcaactgct gccagacggt aagcacgcga tcgggctgga
    14041 agcccaaccg attcatccca ctgttcactt taagataaat atccaacggt gcttttagcc
    14101 gcgcattttg cagtgctttg agctgccagt tgctgtgtac gcaggtggtc aggcggtgct
    14161 ggtcataaat ttccagatcc tgagcatgga aaaatccttc cagcatcagg atcggtcctt
    14221 tccagccgcg ctcacgtaac gttattgcct cttccaggtt aagcaatgca aagccatcgg
    14281 tggccccgag cgcgctccat atacgctcaa taccatgccc gtaagcgttc gcttttacca
    14341 ccgaccagac gcgcgcgtgc ggcgcggcct ggcggacaat ggacagattc tgttttaatg
    14401 cctgcagatc gaggctggcc tgtatcggac gggtcatctc gtttccttag ctgtgtgcgc
    14461 catgtaaatg gcccggacgt gatggcgtaa atccacggct gtagcgcgct acgcttagat
    14521 cctcatatgg gatcgctggc gtgcgaccag agagcagatc gcttaacaac tgaccggaac
    14581 cgcaagccat cgtccagccg agcgtgccgt gaccggtatt tagccacaga tttttaaagc
    14641 gtgtacgccc gacaaccggc gtgccgtctg gcgtcatcgg gcgcagaccc gtccagaaag
    14701 tcgcctgctc gacatgaccg ccgcgtggat agagatcgcg aaccaccatc tccagcgttt
    14761 cacgacgcgg ttgcaacagc tcggtgttaa aaccaacaat ttccgccatc ccgccaacac
    14821 gaatgcggtt atcgaaacgg gtaatggcga ttttgtaggt ttcatcaaga atggtggata
    14881 ccggcgcacc atcttcctgc gcaattggaa tggtcagcga gtagcctttc agcggataca
    14941 ccggaatatt aacaatgcct ttgagcatcg ccgtcgagta agaaccaaac gccatcacat
    15001 acgcatcggc cttaatcact tcatcgccac acttcacgcc gtagatttgc tcgccgtcgc
    15061 aaagcagttg gtcaacgggc gtattaaagc ggaatttaac ccccgcctgc tccgccatcc
    15121 gcgccagatt ctgggtaaat agctgacagt ctccggtttc atcattgggt aactgcaggc
    15181 cgcccgtcag tttgtgcgcc acttctgcca gcgcgggctc cacttccgcc aggcggctgg
    15241 attccagcag ctgatacggt acgccggcat cttccagcac ggcgatatcg cgggtcgcat
    15301 tttcatactg ttgttcggta cggaacagtt gcagcgtccc accctgacgc ccttcatact
    15361 gaatattggt ttcggcgcgc aatgctttca ggcaatcacg gctgtattcc gccagacgca
    15421 ccatccgccc tttgttttcc atgtagtggc tggtgtcgca gttacgtaac atttgccaca
    15481 tccatttcaa ctggaactgc gtaccgtcga gacgaaccgc cagcggcgca tggcgctgga
    15541 acatccattt gatcgctttt aaaggcacac ctggtgccgc ccacggtgca gcatatccgg
    15601 gggagatttg cccggcattc gcggcactgg tttccagggc ggcccccggc tcccgatcaa
    15661 tgacggtgac ctcatgtcct gcctgattta agtaccaggc gctggcaacg cctaccacac
    15721 cacttcccag tatgacaact cgcatagcca ctccgataac ggtagataca gtaaaagaat
    15781 aataatctaa ttacatcttg ataactcagt tgaaaatatt attcaacata cggctttttt
    15841 atggtgagct ggctcacatc tccctgactc tgcggcaata tggcgtcttt agtatctcct
    15901 gctgaacgcc ctttttatcc agcataaaat tccgttcaga agcggattag tggcactctg
    15961 ataataagaa atcgcaaaga aaaaaattct ccggcgacgc ggtttttaac aggtgttcta
    16021 tgcttgaaat gaggtgctcc acacagagag cgccgacaac aatgaggtgc gcgtatggcg
    16081 acgatcgatt ctatgaataa ggacaccaca cgtttgagcg atggacccga ctggacgttc
    16141 gacctgctgg atgtttatct ggcagagata gaccgggtgg cgaaattcta ccggctggat
    16201 acctacccgc accagattga agtgataacc tcagaacaga tgatggatgc ctactccagc
    16261 gtcggcatgc caattaacta tccgcactgg tcattcggta aaaagtttat cgagactgaa
    16321 cggctgtata agcacggtca gcaaggactg gcctatgaaa tcgtcattaa ctctaacccg
    16381 tgtatcgctt acctgatgga agagaacacc attaccatgc aagcgctggt gatggctcat
    16441 gcctgctatg ggcataactc tttcttcaaa aacaattact tattccgtag ctggaccgac
    16501 gccagttcga ttgtcgatta tctgattttt gcccgtaaat atattaccga gtgcgaagag
    16561 cgctatggcg ttgatgaagt agaacggctt ctggactcgt gccacgcgct gatgaactac
    16621 ggcgtggacc ggtacaaacg tccgcaaaaa atctcgctgc aagaagagaa agcccggcag
    16681 aaaagtcgcg aagagtatct gcaaagtcag gtcaatatgc tctggcgtac cctgccgaag
    16741 cgcgaggaag agaaaacggt tgctgaagcg cgccgctatc cgtccgaacc acaagaaaac
    16801 ctgctctatt ttatggagaa aaatgcgcca ctgctggaat catggcagcg tgaaatcctg
    16861 cgtattgtgc gtaaggtgag ccagtatttt tatccgcaaa aacagactca ggtgatgaac
    16921 gaaggctggg ccaccttctg gcactacacc atccttaacc atctgtatga tgaagggaaa
    16981 gtaacggaac gttttatgct ggagtttttg cacagccaca ccaatgtggt cttccagccg
    17041 ccctataaca gcccgtggta cagcggcatc aacccgtatg ccctcgggtt cgccatgttc
    17101 caggatatta aacggatttg tcagtcgcca acggaagaag acaaatactg gttcccggat
    17161 atcgccggtt ccgactggct ggaaacgctg catttcgcga tgcgtgattt caaagatgag
    17221 agttttatca gccagttcct gtcaccgaaa gtgatgcgtg atttccgctt cttcaccgtg
    17281 ctggatgacg atcggcataa ttatctggag atttccgcta ttcataatga agaaggttat
    17341 cgggagatcc gtaaccggtt atcgtcgcaa tataacttaa gtaatctgga gccgaatatt
    17401 cagatctgga acgtggattt gcgcggcgac cgttcgctga cgctgcgtta cattccacat
    17461 aatcgcgcac cgctggatcg ggggcgcaaa gaagtactga agcatgtgca tcgcctgtgg
    17521 ggatttgatg tgatgctgga acagcaaaac gaagacggca gcgtcgagtt gctggaacgt
    17581 tgcccgccaa gaatgggaaa tctgtaaaaa caacaaaaaa cccctcgttt gaggggtttg
    17641 ctttttaaac ggaagggatt atcgcccctg aatggctaaa tcacccggca gatttttctg
    17701 catccggtgc caaatctcgc cactctcatg cccatagcga cgcactgttt cgtacacctg
    17761 atcgtgcgcg ccttcactgc acaacgccga cagtttgtgg tagaagccca gcgccagact
    17821 gcgcgcttcc ggattggcga aatagtgacg accaatgcgc gtatacagtc ctttcatccc
    17881 gttaaggatc agaccgtaaa tcgggttgcc ggaagcaaac gccaggccgc ggaatatgtt
    17941 gtaatccagc tcggcaaagg catcggcgtg atcggccact tcattagcgg tagccagcac
    18001 ttcctgcgct ttatcgggat gctgacgaaa cgcggtgcga ataaaaatag ttgaaatatt
    18061 ggtacgcacc gacagcaaat tatcaataag ctgcggcaca ctttcgtgat ccagtcgcgc
    18121 cagtgtttca aggatattta aaccagaggt ttcccagaaa ttattcacct tcgtcggctt
    18181 gccatgttga atggtcaacc agccatctcg tgccagacgc tgtaacactt cacgtaacgt
    18241 agtacgcgtt acgccaatta attctgaaag ttcacgttct gcgggcaaaa tagtcccggg
    18301 agggaagcgg ttattccaga tactttcaat aatgtactct tccgcgaaac ccgccgggct
    18361 ttgcgcctta atgaccatag tgagatttcc ataacacagc aaaacaaagt tggactcatc
    18421 ataccagagg gctaactacg ctgatagcag acaaaaagaa gaaaaaaggg atcaaggctt
    18481 cattttccgc atctctttcc ccttgactat caagacttgc ctcactcctg cctgaccgtt
    18541 aagctttgcg tttaaacata aaaacatcat tgattatttg taaggtaagg gaaaccatca
    18601 tggagatctc ctggggccgc gcgctatggc gcaatttttt gggccagtcc cccgactggt
    18661 acaaactcgc cctcatcatt ttcttaatcg taaacccgtt aattttcctc atcagccctt
    18721 tcgtcgctgg ctggttgctg gtagcggaat ttattttcac tctggcgatg gccctgaaat
    18781 gctacccgct actccccggt ggtctgttgg ctatcgaagc ggtattcatc ggcatgacca
    18841 gcgcggaaca cgtccgtgaa gaggtggcgg caaatcttga agtcttgctg ttattgatgt
    18901 ttatggtggc gggtatctat tttatgaaac agctgttgct gttcatattt acccgtctgc
    18961 tgttaagcat tcgctccaaa atgctgctgt cgctctcttt ttgcgtggcg gcagcgttcc
    19021 tctccgcgtt cctcgatgcc ttaaccgtcg tggcggtggt gatcagcgtt gccgtcggtt
    19081 tttatggtat ttatcatcgc gtagcctctt cccgtaccga agacaccgac ctgcaagacg
    19141 atagtcatat cgacaagcat tacaaagtgg ttctggaaca gttccgtggc tttctgcgta
    19201 gcctgatgat gcatgccggt gtcggcaccg cattaggcgg cgtaatgacc atggtgggcg
    19261 aaccacagaa cctgatcatc gctaaagcgg ctggctggca ttttggcgat ttcttcctgc
    19321 gcatgtcgcc ggtgaccgtt ccggttctga tttgtggcct gttaacctgc ctgctggtag
    19381 agaagctgcg ttggtttggc tacggtgaaa cgctgccgga gaaagtccgc gaagtgctgc
    19441 aacagtttga cgatcaaagc cgccaccagc gtacccgtca ggataaaatt cgtctgattc
    19501 tccaggcgat tattggcgtc tggctggtga ctgcgctggc gttgcatctg gcggaagttg
    19561 gtttgattgg tttgtcagtc attattctgg cgacatcatt gaccggtgtc accgatgaac
    19621 atgctatcgg taaagccttc accgaatctc tgccattcac cgcactgttg acggtgtttt
    19681 tctcggtagt cgcggtgatt atcgaccaac aactgttttc gccgattatt cagtttgtgt
    19741 tgcaggcatc ggaacatgct cagctgtcgt tgttctatat tttcaacgga ctgctgtcat
    19801 ccatttcgga taacgtcttc gtggggacga tttatatcaa cgaagcgaaa gcggcaatgg
    19861 aaagtggcgc tatcacgttg aagcaatacg agctgctggc ggtcgccatt aataccggta
    19921 ccaatctgcc ctctgtcgct acgccgaacg gtcaggctgc gttcctgctg acctctgcac
    19981 tcgcgccatt gattcgcctc tcttatggcc gcatggtgtg gatggccctg ccttacaccc
    20041 tcgtcctgac actcgtcgga ctgctttgcg tcgagtttac gcttgcccct gtaaccgaat
    20101 ggtttatgca aatgggctgg atagcaacgc tttgataaca acttaccggg catattaatg
    20161 cccggtttgc cttttcgccc gataattgtc caattgcgtc ctatttttgc tgcctcctgg
    20221 tggcgcagcg aatgaattgg tttaaactgc gcactctatg catattgcag ggaaatgatt
    20281 atgttgcgat ttttgaacca atgttcacaa ggccggggcg cgtggctgtt gatggcgttt
    20341 actgctctgg cactggaact gacggcgctg tggttccagc atgtgatgtt actgaaacct
    20401 tgcgtgctct gtatttatga acgctgcgcg ttattcggcg ttctgggtgc tgcgctgatt
    20461 ggcgcgatcg ctccaaaaac gccactgcgt tatgtagcga tggttatctg gttgtatagt
    20521 gcgttccgcg gtgtgcagtt aacttacgag cacaccatgc ttcagctcta tccttcgccg
    20581 tttgcgacct gtgattttat ggttcgtttc ccggaatggc tgccgctgga taaatgggtg
    20641 ccgcaagtgt ttgtcgcctc tggcgattgc gccgagcgtc agtgggattt tttaggtctg
    20701 gaaatgccgc agtggctgct cggtattttt atcgcttacc tgattgtcgc agtgctggtg
    20761 gtgatttccc agccgtttaa agcgaaaaaa cttgatctgt tcggtcgcta atccattcgg
    20821 cgctcctgcg ggagcgcttt tttcctgccg ctatatttat ttgacccgca gtaaatcaga
    20881 acttcgcgtt gtataacgtg gtgaaagcat ggctcgcttc atctgccatt gttgctggat
    20941 cccctgcccg gcaaaataga gtgttcctct gccctcttta gcattcagtg tatccattac
    21001 cgccatcaat tgctcactcc cggggcgcgg agcgttgtca tcgaataaat tgagctgcgc
    21061 gactccctga ctgaagaaat cccccagcat cacgcccgct ttttggtagc gatggcccgc
    21121 ttgccagatg gcatccagag atcgcgtagc ggcgttaatg atatccctgc tgtcctgagt
    21181 gggcgtcagc agttttaccg acgcgctatt gccgtaataa ggttcattga gcgtaaatgg
    21241 tgacgtctta ataaatgtgg agataaaccg acaatattga tgctcactgc gaagtttttc
    21301 cgccgcccgg gcagcgtaac tacaaatggc ctgccgcatc gacgtataat ccgtgatgcg
    21361 ttcaccaaac gagcgggaac agataatttc ctgctttgtc ggtgcaaatt cttccagttg
    21421 caaacagggt tcaccgcgca gttcacgcac cgttctttcg agcacgacat taaaatgttt
    21481 acggataaac cggatatctg tatccgccaa atcgagaacg gttttgatcc ccatcgcgtc
    21541 cagttttttg ctgatccgcc gtccaatccc ccagacgtca tccacgggga gagcagacat
    21601 taatttacgc tggcgttcca gatttgataa atccaccacc ccacccgtct gccgctgcca
    21661 tttttttgcc gcatgattag caagcttagc cagcgttttg gtctgggcga tccccacacc
    21721 aacagtaaga tgggtacgtt gtagcaccgt tgcgcgaatt tctctgccaa agtcagtcag
    21781 atcgcgacaa ttacgcacac ctgtcagatc gcagaatgcc tcatcaatac tgtaaatctc
    21841 gacgcggggc gatagttctt ccagcgtcga catcacccga ttgctcatgt ctgcgtaaag
    21901 ctcataattg ctgctaaagc aaaccacgcc acagcgacga aacagatctt tttgtttgaa
    21961 ccagggatcg cccattttaa cgccaagcgc ctttgcctca gcgtttcggg cgataacgca
    22021 accgtcatta ttcgatagca caaccaccgg tttaccccat aaatcagggc gaaacaccgt
    22081 ctcacagctg gcataaaacg cgtttacatc acagagggca aacatcagcg catcgcctta
    22141 acgacgtgga tcaccacacc aaagacatcc agcgtatctt cactactgat ggtaatgggc
    22201 gagtacgcgc tgttcatggg aataagctgt accgtcgggc gtagttgcaa ttttttcacc
    22261 gtaaactcgc cgtcaacagc agcgatgaca atatcaccat ggctggcggt aatagcgcta
    22321 tcgacaatca gtaaatcacc gtcactaatt ccaccatcaa tcatagaatc accacttgct
    22381 ttgacgaagt aagtcgcgct gggatgctgg atcaacagtt gattcagatc gatgcgctgt
    22441 tcaacgtaat ctgcggccgg tgaaggaaag ccacactgaa caagatcgct aaatagcgga
    22501 aaagtcacaa tttcgcggag atccgcaggc ttgataaaca acataataat ctgcctgaag
    22561 ttatactgtt tttatataca gtagtctgtt cttgccagca gatcaatact gattcaggct
    22621 atcaatattt gtcgctgcat agactgctga tttttcgttc tcttatcctg tgctcacgcg
    22681 gccttctggc gacgacgctc atccagcaga aatgaaaaat atcacccggc taaaaaatag
    22741 aatagaagca ttcgcc
//
LOCUS       DAZFFG010000078        22687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFFG010000078 DAZFFG010000000
VERSION     DAZFFG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.078"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1326)
                     /gene="djlB"
                     /locus_tag="ACHJ7J_003276"
     CDS             complement(<1..1326)
                     /gene="djlB"
                     /locus_tag="ACHJ7J_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO5001467.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHDILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMP"
     gene            complement(1323..2030)
                     /gene="ybeR"
                     /locus_tag="ACHJ7J_003277"
     CDS             complement(1323..2030)
                     /gene="ybeR"
                     /locus_tag="ACHJ7J_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5001468.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWQQ
                     YFAGYTFGRQYWQSPEDDNLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2194..3171
                     /gene="ybeQ"
                     /locus_tag="ACHJ7J_003278"
     CDS             2194..3171
                     /gene="ybeQ"
                     /locus_tag="ACHJ7J_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO5001469.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3241..3723)
                     /gene="ybeL"
                     /locus_tag="ACHJ7J_003279"
     CDS             complement(3241..3723)
                     /gene="ybeL"
                     /locus_tag="ACHJ7J_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO5001470.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            3958..6540
                     /gene="leuS"
                     /locus_tag="ACHJ7J_003280"
     CDS             3958..6540
                     /gene="leuS"
                     /locus_tag="ACHJ7J_003280"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO5001471.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDSEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6555..7136
                     /gene="lptE"
                     /locus_tag="ACHJ7J_003281"
     CDS             6555..7136
                     /gene="lptE"
                     /locus_tag="ACHJ7J_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO5001472.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7136..8167
                     /gene="holA"
                     /locus_tag="ACHJ7J_003282"
     CDS             7136..8167
                     /gene="holA"
                     /locus_tag="ACHJ7J_003282"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO5001473.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAE
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     LLADVFIDG"
     gene            8169..8810
                     /gene="nadD"
                     /locus_tag="ACHJ7J_003283"
     CDS             8169..8810
                     /gene="nadD"
                     /locus_tag="ACHJ7J_003283"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO5001474.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            8834..9445
                     /gene="cobC"
                     /locus_tag="ACHJ7J_003284"
     CDS             8834..9445
                     /gene="cobC"
                     /locus_tag="ACHJ7J_003284"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO5001475.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESIWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9705..10022
                     /gene="rsfS"
                     /locus_tag="ACHJ7J_003285"
     CDS             9705..10022
                     /gene="rsfS"
                     /locus_tag="ACHJ7J_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO5001476.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10026..10493
                     /gene="rlmH"
                     /locus_tag="ACHJ7J_003286"
     CDS             10026..10493
                     /gene="rlmH"
                     /locus_tag="ACHJ7J_003286"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO5001477.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10524..12425
                     /gene="mrdA"
                     /locus_tag="ACHJ7J_003287"
     CDS             10524..12425
                     /gene="mrdA"
                     /locus_tag="ACHJ7J_003287"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO5001478.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12428..13540
                     /gene="mrdB"
                     /locus_tag="ACHJ7J_003288"
                     /gene_synonym="rodA"
     CDS             12428..13540
                     /gene="mrdB"
                     /locus_tag="ACHJ7J_003288"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO5001479.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13551..14639
                     /gene="rlpA"
                     /locus_tag="ACHJ7J_003289"
     CDS             13551..14639
                     /gene="rlpA"
                     /locus_tag="ACHJ7J_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO5001480.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14779..15990
                     /gene="dacA"
                     /locus_tag="ACHJ7J_003290"
     CDS             14779..15990
                     /gene="dacA"
                     /locus_tag="ACHJ7J_003290"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO5001481.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16100..16363
                     /gene="ybeD"
                     /locus_tag="ACHJ7J_003291"
     CDS             16100..16363
                     /gene="ybeD"
                     /locus_tag="ACHJ7J_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO5001482.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16464..17105
                     /gene="lipB"
                     /locus_tag="ACHJ7J_003292"
     CDS             16464..17105
                     /gene="lipB"
                     /locus_tag="ACHJ7J_003292"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO5001483.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17365..18318
                     /gene="ybeF"
                     /locus_tag="ACHJ7J_003293"
     CDS             17365..18318
                     /gene="ybeF"
                     /locus_tag="ACHJ7J_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO5001484.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            18526..19491
                     /gene="lipA"
                     /locus_tag="ACHJ7J_003294"
     CDS             18526..19491
                     /gene="lipA"
                     /locus_tag="ACHJ7J_003294"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO5001485.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(19592..19795)
                     /gene="tatE"
                     /locus_tag="ACHJ7J_003295"
     CDS             complement(19592..19795)
                     /gene="tatE"
                     /locus_tag="ACHJ7J_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO5001486.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(19924..20712)
                     /locus_tag="ACHJ7J_003296"
     CDS             complement(19924..20712)
                     /locus_tag="ACHJ7J_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO5001487.1"
                     /translation="MLAAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            20805..21188
                     /gene="crcB"
                     /locus_tag="ACHJ7J_003297"
     CDS             20805..21188
                     /gene="crcB"
                     /locus_tag="ACHJ7J_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO5001488.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21242..21451)
                     /gene="cspE"
                     /locus_tag="ACHJ7J_003298"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21242..21451)
                     /gene="cspE"
                     /locus_tag="ACHJ7J_003298"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5001489.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(21626..22186)
                     /gene="pagP"
                     /locus_tag="ACHJ7J_003299"
     CDS             complement(21626..22186)
                     /gene="pagP"
                     /locus_tag="ACHJ7J_003299"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO5001490.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKAFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5813 a         5683 c         5597 g         5594 t
ORIGIN      
        1 gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa aattcgccgc
       61 ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatttt ctgcatcaag
      121 acggcttacg atcggcatta atgattcttc tgttggttgg ctggcctgca ctaattgttt
      181 ggcaactaaa tataatggcc ataagggatg ttctgtatca cgcgtgagtg agatccgcgg
      241 cttacgtcct ttgtgcaaaa ccatctcgac cagagaacgc cccagcgcag agagtttttc
      301 atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg ggatataaag
      361 tagcggtttt ccctgatcgt taaccagttc acgagcttca atcatcatca ccactaacgg
      421 gtaataatcg acccgatgct gacggcacca ttgcaatatt aaagcatccg cctgggtgga
      481 gggatactcg cgccagtagt cacaaagttc tgccagcaag ctttcgcctt ctccacaata
      541 gacgcgttgt gcgtattcat aataacgcgc atcttcatta tcagattcag cttcccgcca
      601 cgaccgggcg taatccagga gttcgggaat tcccaggcga cctgcgctat accagcggag
      661 taaatttctg tgcagttttg catcatcggg gataatcact gcgcgatgtt gcgctaaata
      721 caatgtcagc cattctggat gcgatgacca aatccgagcg aacatttggt aaaaatcgat
      781 ggtgtcattt tgcacggcaa cgggcagatg cagaatggtc tggaagttaa aaacattccc
      841 cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc cgacgttttc
      901 ctcctgcaac caccgcaatc tttctgctaa taacaccacg cactcgaagg ataaatgggc
      961 agtgttcatg gcgattgtgc acagcgacca gcgtaattca tcaatctctt ccatcgagca
     1021 ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt cgctatcaga
     1081 ggcaagtaac gcgcgaaagg cgagcagaat atcatgttct gctacctcat attcttctgg
     1141 ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcctcctcat acgcctgacg
     1201 aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca gcgccagata
     1261 agcgcggcgg ataatatcga cgtcagtcgt ttcctctatt tcgaggatct tccagcaatt
     1321 tttcataggt cgctcatatc cttaagcgtt tccgggcatg cagaatagta agccaatggt
     1381 tgccaggaca aggtagggta atacgcatca tcactggcaa ataattgata aaacatatca
     1441 ttgccggaat cgtcgtattc tttacgcgct aagaattcac gtaataactg aagattatca
     1501 tcttcggggg actgccaata ttgccgacca aaggtataac cggcaaagta ttgctgccag
     1561 ttactataat aacgtagcgc ccgtagatga atgcgcgact ggatccatag actttcttct
     1621 tcgctcaacc agttattaag tacgcccatt cggcttaaaa aacccatccg gacataatcc
     1681 caggctttga ttcccccttc gccacagcat tcggcggtgc tggcgacaaa ttgcgcataa
     1741 gcctgacctt gctcatttag ttcagcaaga tagtcacgcc attcacatgg agaataacga
     1801 aaccagcggt gataaaaccc tgccaaccga gcggcatgac cgttatcgat cattcggtga
     1861 attgtgcgca gcaggtcatc acggtcatca attccccagg aggaagaaag gtcaaaaaag
     1921 catgtaccta aatacatttt gggggatgaa tgcgaaggta aacagcattc attgcgtatt
     1981 tccattggag tggaaagtgc aaacaatatt ttttgcgact ccatgtccat ataatttatc
     2041 cttaaacatt catttaatga atgtaaacta cattacgagg ctcatttatt tatacttttc
     2101 tgacgcaaca taatacgcat aaaataaggc ttacgcaaaa atggaacaca taaaaatata
     2161 atatgcttta tctgcaacca taaggaaatg ataatgattt ttacgtcaag ttgctgcgat
     2221 aatttatcaa tagatgagat tatcgaacgt gctgaaaaag gcgattgcga ggctcaatat
     2281 attgttgggt tttattataa tcgcgatagc gcaattgact ctccggacga cgaaaaggcc
     2341 ttttactggc tgaagctggc cgctgagcaa ggtcattgtg aagcacagta ttccttaggg
     2401 caaaagtata ccgaggataa aagccgtcat aaagataatg agcaagccat cttctggctg
     2461 aaaaaagctg ccctacaagg ccatactttc gcttccaacg cccttggctg gatactggat
     2521 cgtggagaag accccaacta taaagaagcg gttgcctggt atcagatagc cgcggagagc
     2581 ggaatgtctt atgcgcaaaa taatcttggg tggatgtaca gaaatggcaa cggagtcgca
     2641 caagactata cgctggcatt tttctggtac aaacaagctg cattacaagg ccatagtgac
     2701 gcgcaaaaca atctggccga tctttatgaa gacggaaaag gcgttgctca aaacgagaca
     2761 ctcgccgcat tctggtattt gaaaagcgca cagcagggta atcggcatgc tcagtttcaa
     2821 attgcatggg attataacgc tggcgaaggg gtggaccagg actataagca agcgatgtac
     2881 tggtatctga aggctgccgc tcaggggagc gtcggcgctt acgtcaacat cggttatatg
     2941 tataaacacg gacaaggcgt tgagaaagat tatcaggctg cctttgaatg gtttacgaaa
     3001 gccgctgaat gcaatgacgc cactgcctgg tataacctgg ccattatgta tcactacgga
     3061 gaaggaagac ctgtcgatct ccgacaggct ctcgacctgt atcgtaaagt tcagtcatcc
     3121 ggaaccagag atgtcagtca ggaaattcgt gagactgaag atttactgta gagataaaaa
     3181 caagggccgg ggaatttcat tcggcccata ttctctttat cgccgtgcaa aatcagacta
     3241 ttacggctcg aacgggcgtc tctggaactg gtcatgacca catttcgggc atagcgttag
     3301 cacttccggt gtgtagatcg ggagatggaa gtgacatttc tcgcagacca gatttcccag
     3361 cccgaccact tctccgctgt gataaacccc atgatgattg aggtcctgga aaacttcgcg
     3421 ccattcaagc tgcgttttat cggtgatgtc tgccagctct tgccacaagc tttctttaat
     3481 cacccgcata aagacgctgt cagattcttc tttcaggctc tcttcatagc tcatggcgaa
     3541 ctcttccagg tcacgtctga cagctcgcgt cagctcatcg acctcggttc gcgttaactc
     3601 ccctgttttt attacgcgct cgcgcgcctg ttccaccagt gcgtcgatat cacgttcgcc
     3661 attgcgcagg cgttcgctca gtgacgcaac cagttcacgg taatattgag caaccttgtt
     3721 catcgtttca tctcccgggt cgttaactga ctataataat agacgttctt tgttccttgc
     3781 tttgctaata cgcccacaaa ctctgtaaat taatgtatga ataatccggg catcatttca
     3841 acgtcgagtg cgcgaaaggc tgttttgacg cggacgtttg ggctatgcta tgcggatctg
     3901 aaaaaccacc tcaacgctac atttgtagcc gtattgaaaa caggaccact ggctgccatg
     3961 caagagcaat accgcccgga agagatagaa tccaaagtac agcttcattg ggatgagaag
     4021 cgcacatttg aagtaaccga agacgagagc aaagagaagt attactgcct gtctatgctt
     4081 ccctatcctt ctggtcgact acacatgggc cacgtacgta actacaccat cggtgacgtg
     4141 atcgcccgct accagcgtat gctgggcaaa aacgtcctgc agccgatcgg ctgggacgcg
     4201 tttggtctgc ctgcggaagg cgcggcggtg aaaaacaaca ccgctccggc accgtggacg
     4261 tacgacaaca tcgcgtatat gaaaaaccag ctcaaaatgc tgggctttgg ttatgactgg
     4321 agccgcgagc tggcaacctg tacgccggaa tactaccgtt gggaacagaa attcttcacc
     4381 gagctgtata aaaaaggcct ggtatataag aagacttctg cggtcaactg gtgcccgaac
     4441 gaccagaccg tactggcgaa cgaacaagtt atcgacggct gctgctggcg ctgcgatacc
     4501 aaagttgaac gtaaagagat cccgcagtgg tttatcaaaa tcactgctta cgctgacgag
     4561 ctgctcaacg atctggataa actggatcac tggccagaca ccgttaaaac catgcagcgt
     4621 aactggatcg gtcgttccga aggcgtggag atcaccttca acgttaacga ctatgacaac
     4681 acgctgaccg tttacactac ccgcccggac acctttatgg gttgtaccta cctggcggta
     4741 gctgcgggtc atccgctggc gcagaaagcg gcggaaaata atcctgaact ggcggccttt
     4801 attgacgaat gccgtaacac caaagttgcc gaagctgaaa tggcgacgat ggagaaaaaa
     4861 ggcgtcgata ctggctttaa agcggttcac ccattaacgg gcgaagaaat tcccgtttgg
     4921 gcagcaaact tcgtattgat ggagtacggc acgggcgcag ttatggcggt tccggggcac
     4981 gaccagcgcg actacgagtt tgcctctaaa tacggcctga acatcaaacc ggttatcctg
     5041 gcggctgacg gctctgagcc agatctttct cagcaagccc tgactgaaaa aggcgtgctg
     5101 ttcaactctg gcgagttcaa cggtcttgac catgaagcgg ccttcaacgc catcgccgat
     5161 aaactgactg cgatgggcgt tggcgagcgt aaagtgaact accgcctgcg cgactggggt
     5221 gtttctcgtc agcgttactg gggcgcgccg attccgatgg tgacgctgga agacggtacc
     5281 gtaatgccga ccccggacga ccagctgccg gtgatcctgc cggaagatgt ggtgatggac
     5341 ggcataacca gcccgattaa agcagatccg gagtgggcaa aaactaccgt taacggtatg
     5401 ccagcactgc gtgaaaccga cactttcgac acctttatgg agtcctcctg gtactatgcg
     5461 cgctacactt gcccgcagta caaagaaggt atgctggatt ccgaagcggc taactactgg
     5521 ctgccggtgg atatctacat tggtggtatc gaacacgcca ttatgcacct gctctacttc
     5581 cgcttcttcc acaaactgat gcgtgatgca ggcatggtga actctgacga accagcgaaa
     5641 cagttgctgt gtcagggtat ggtgctggca gatgccttct actatgttgg cgaaaacggc
     5701 gaacgtaact gggtttcccc ggttgatgct atcgttgaac gtgacgagaa aggccgtatc
     5761 gtgaaagcga aagatgcggc aggccatgaa ctggtttata ccggcatgag caaaatgtcc
     5821 aagtcgaaga acaacggtat cgacccgcag gtgatggttg aacgttacgg cgcggacacc
     5881 gttcgtctgt ttatgatgtt tgcttctccg gctgatatga ctctcgaatg gcaggaatcc
     5941 ggtgtggaag gggctaaccg cttcctgaaa cgtgtctgga aactggttta cgagcacaca
     6001 gcaaaaggtg atgttgcggc actgaacgtt gatgcgctga ctgaagatca gaaagcgctg
     6061 cgtcgcgatg tgcataaaac gatcgctaaa gtgaccgatg atatcggccg tcgtcagacc
     6121 ttcaacaccg caattgcggc gattatggag ctgatgaaca aactggcgaa agcaccgacc
     6181 gatagcgagc aggatcgcgc tctgatgcag gaagcgctgc tggccgttgt ccgtatgctt
     6241 aacccgttca ccccgcacat ctgcttcacg ctgtggcagg aactgaaagg cgaaggcgat
     6301 atcgacaacg cgccgtggcc ggttgctgac gaaaaagcga tggtggaaga ctccacgctg
     6361 gttgtggtgc aggttaacgg taaagtccgt gccaaaatca ccgttccggt ggacgcaacg
     6421 gaagaacagg ttcgcgaacg tgctggccag gaacatctgg tagcaaaata tcttgatggc
     6481 gttactgtac gtaaagtgat ttacgtacca ggtaaactcc tcaatctggt cgttggctaa
     6541 gcgcgggagg aagcgtgcga tatctggcaa cattgttgtt atctctggcg gtgttaatca
     6601 ccgccgggtg tggctggcat ctgcgtgata ccacgcaggt tccttccact atgaaggtca
     6661 tgatcctgga ctcaggtgat ccgaacgggc cattaagccg tgcggtgcgt aaccagttac
     6721 gtctgaatgg tgtcgagttg cttgataaag aaaccacgcg taaggacgtt ccatccctgc
     6781 gtttgggtaa agtgagcatc gcgaaagata ccgcatcggt attccgtaac ggtcaaacag
     6841 cagagtatca gatgatcatg acggtgaatg cgaccgtgtt gatccccggc cgtgatatct
     6901 acccgattag cgccaaagtc ttccgttcgt tctttgataa cccgcaaatg gcgttagcga
     6961 aagataacga acaagacatg atcgtaaaag agatgtacga ccgtgctgcc gaacagctga
     7021 ttcgtaagct gccaagcatc cgtgctgcgg atattcgttc cgacgaagaa cagacgtcga
     7081 ctacaacgga tactccggca acacctgcac gcgtctccac cacgctgggt aactgatgat
     7141 tcggttgtac ccggaacaac tccgcgcgca gctcaatgaa gggctgcgcg cggcgtatct
     7201 tttacttggt aacgatcctc tgttattgca ggaaagccag gacgctgttc gtcaggtagc
     7261 tgcggaacaa ggattcgaag aacaccacac tttttccatt gatcccaaca ctgactggaa
     7321 tgcgatcttt tcgttatgcc aggccatgag tctgtttgcc agtcgacaaa cgctattgct
     7381 gttgttacca gaaaacggac cgaacgcggc gatcaatgaa caacttctca cactcaccgg
     7441 acttctgcat gacgacctgc tgttgatcgt ccgcggtaat aaattaagca aagcgcaaga
     7501 aaatgccgcc tggtttactg ctcttgcgaa tcgcagcgtg caggtgacct gtcagacacc
     7561 ggagcaggct cagcttcccc gctgggttgc tgcgcgcgca aaacagctca acttagaact
     7621 ggatgacgct gcaaatcagg tgctctgcta ctgttatgaa ggtaacctgc tggcgctggc
     7681 ccaggcactg gagcgtttat cgctgctctg gccagacggc aaactgacat taccgcgcgt
     7741 tgaacaggcg gtgaatgatg ccgcgcattt cacccctttt cattgggttg atgctttgtt
     7801 gatgggaaaa agtaagcgcg cattgcatat tcttcagcaa ctgcgtctgg aaggcagcga
     7861 accggttatt ttgttgcgca cattacaacg tgaactcttg ttactggtta acctgaaacg
     7921 ccagtctgcc catacgccac tgcgtgcgtt gtttgataag catcgggtat ggcagaaccg
     7981 ccggggcatg atgggcgagg cgttaaatcg cttaagtcag ccgcagttac gtcaggccgt
     8041 gcaactcctg acacgaacgg aactcaccct caaacaagat tacggtcagt cagtgtgggc
     8101 agagctggaa gggttatctc ttctgttgtg ccataaactc ctggcggacg tatttatcga
     8161 cggttgatat gaaatcttta caggctctgt ttggcggcac ctttgatccg gtgcactatg
     8221 gtcatcttaa acccgtggaa acgctggcga atttgattgg tctgacgcgg gtcacaatca
     8281 tccctaataa tgttcctccg catcgtcccc agccggaagc gaacagcgtg cagcgtaaac
     8341 acatgcttga actggcgatt gccgacaagc cattatttac tcttgatgaa cgcgagctaa
     8401 agcgcaatgc cccctcttac actgcgcaaa cactgaaaga gtggcggcag gaacaaggac
     8461 cggacgtgcc gctggcgttt attattggtc aggattcatt gctgaccttt ccgacctggt
     8521 acgaatacga aacgatactc gacaatgcac atttgatcgt ctgtcggcgt ccaggttacc
     8581 cacttgaaat ggcgcaaccg caataccagc aatggctgga agatcatttg acacataacc
     8641 cggaagatct tcaccttcag cctgccggta aaatttatct ggctgaaacg ccgtggttta
     8701 acatctcggc gaccatcatc cgtgaacgtt tgcaaaacgg tgaatcatgt gatgatttat
     8761 tgccggaacc ggtactgact tacattaacc aacaaggctt gtatcgctga tacctgtcgt
     8821 tacggagggc atgatgcgac tgtggttaat tcgtcatggc gaaacacaag cgaatgtcga
     8881 tggtctgtac agcggtcatg cgcccacacc cctgaccgca cgcggtattg agcaagcgca
     8941 aaatctgcat acactgctag atgatgtttc ctttgacctg gttttatgca gtgaactgga
     9001 acgggcacag cataccgcgc gactggttct cagtgaccgc cagctccccg ttcatatcat
     9061 ccctgaactc aacgaaatgt tttttggcga ctgggagatg cgacatcatc gcgacctcat
     9121 gcaagaagat gccgaaaact atagcgcgtg gtgcaatgac tggcagcatg ctatccccac
     9181 gaacggtgaa ggatttcagg cattttcgca acgtgtggaa cgctttatcg caaggcttag
     9241 tgaatatcag cactatcaga atattttaat cgtcagccat cagggcgtac tgagtctgtt
     9301 aattgctcgt ttaattggca tgcctgccga gtctatatgg cattttcgcg ttgaccaggg
     9361 gtgttggagc gccattgata tcaaccaaaa attcgcgacg ctacgcgtcc tcaatagccg
     9421 tgccatcggg gtcgagaatg catgactttt ctgttttttt acgggtaagc cgcaacgacc
     9481 attgacagcc ccgggcaggc tgatattctc cgcagccaga ctttttccgc cagacacgac
     9541 tttgtagaaa ttgttttaca aaaatggcga tgcaatctgc ggcgcggggt gggatgatag
     9601 cccactttcg aaagccgatt cggcgacaat tgtcccgaaa tcgcctctgg ttcaggtata
     9661 ctgacagacc atttttatct atttgattca cccaggggga aaacttgcag ggtaaagcac
     9721 tccaggattt tgttatcgac aaaattgatg acctcaaagg tcaggacatc atcgccttag
     9781 acgttcaggg caaatccagc atcactgact gcatgatcat ctgtacgggt acgtccagcc
     9841 gtcatgttat gtccattgct gaccacgttg tgcaggagtc tcgcgcagcg ggcctgttac
     9901 cgctcggcgt agaaggtgaa aacagcgccg actggattgt cgtggatttg ggcgatgtga
     9961 ttgtccatgt catgcaggaa gagagccgtc gcctgtatga actggaaaaa ctctggagtt
    10021 aatgcgtgaa gctgcaactt gtcgccgtgg gaacgaaaat gccggactgg gtacaaaccg
    10081 gttttaccga gtacctgcgt cgttttccga aagatatgcc cttcgagctg attgaaattc
    10141 cggccggaaa acgcggcaag aatgcggaca tcaagcgcat actcgacaaa gagggtgagc
    10201 agatgttggc ggccgcaggc aaaaaccgca ttgtcaccct cgatattcca ggcaagccct
    10261 gggatacgcc gcagttagcc gctgagctgg aacgctggaa gctggatggt cgcgacgtca
    10321 gtctactgat tggcgggcct gaagggttgt cgcctgcctg taaagcggcg gctgagcaga
    10381 gctggtcgct gtcggcgctt accctccccc atccgctggt tcgcgtgctg gtcgcagaga
    10441 gtctgtaccg ggcgtggagc atcaccacca accatcctta tcaccgtgag tgataaggga
    10501 gctttgagta gaaaacgcag cggatgaaac tacagaactc ttttcgcgac tatacggcag
    10561 agtccgcgct gtttgtgcgc cgggcgctgg tcgccttttt ggggattttg ctgctgaccg
    10621 gcgtgcttat cgccaacctg tataatctgc aaattgttcg ctttaccgac taccagaccc
    10681 gctctaatga aaaccgcatt aagctggtgc ctatcgcgcc cagccgcggc attatctatg
    10741 accgtaacgg tatccctctg gccctcaacc gcactatcta ccagatagaa atgatgccgg
    10801 agaaagtcga taacgtgcag caaactctgg acgctttgcg cagcgtggta gatctgaccg
    10861 atgacgatat tgctgcattc cgaaaagagc gcgcacgttc acaccgtttc acctctattc
    10921 cggtgaaaac taacctgacc gaagtacaag tagctcgctt tgccgtcaat cagtaccgtt
    10981 ttccgggtgt tgaagttaaa ggctataaac gtcgttacta tccttacggt tcggcgttga
    11041 cccacgtcat cggctatgtg tcgaaaatca acgataaaga cgtcgaacgc ctgaataatg
    11101 acggcaaact ggccaactat gcggcaacgc atgatatcgg taagctgggc attgagcgtt
    11161 actatgaaga cgtgctgcac ggtcagaccg gttatgaaga ggttgaagtt aacaaccgtg
    11221 ggcgtgttat ccgccagtta aaagaagtgc caccgcaagc cggacacgat atttacctga
    11281 cgctggatct caaactccag caatatattg aaacgctgct ggcgggtagc cgtgcagctg
    11341 tggtagtcac cgatccgcgt acaggtgggg tgctggcgct ggtttccacg cctagttatg
    11401 acccaaactt gtttgttgac ggtatctcca gcaaagatta ttccgccttg ttgaacgacc
    11461 cgaatacacc gctggtgaac cgcgccacac agggggttta tcctcccgcg tctacagtta
    11521 aaccctatgt ggcggtttcg gcattgagcg ccggggtgat cacgcgcaat acgacgctgt
    11581 ttgacccagg ctggtggcaa ctgccaggtt cggaaaaacg ttatcgtgac tggaaaaaat
    11641 ggggccacgg gcgtctgaat gtcacaagat cgctggaaga atctgcggat accttcttct
    11701 atcaggtggc ctacgatatg gggatcgatc gcctctccga atggatgggt aaattcggtt
    11761 atggtcatta caccggtatc gacctggcgg aagaacgttc cggcaacatg cctacccgcg
    11821 aatggaaaca gaaacgcttt aaaaaaccgt ggtatcaggg tgacaccatt ccggttggta
    11881 tcggtcaggg ttactggaca gcgaccccaa tccagatgag taaggcactg atgatcctga
    11941 ttaatgacgg tatcgtgaag gttccgcatt tgctgatgag caccgctgaa gacggcaaac
    12001 aggtgccatg ggtacaaccg catgaaccgc ccgttggcga tattcattcc ggttactggg
    12061 agctggcgaa agacggtatg tacggtgttg ctaaccgccc taacggtacg gcgcataaat
    12121 actttgccag cgcaccgtac aaaattgcgg cgaaatctgg taccgctcag gtcttcggtc
    12181 tgaaagcgaa cgaaacctat aatgcgcaca aaattgccga gcgtttacgt gaccacaaac
    12241 tgatgaccgc ctttgcgcct tacaacaatc cgcaagtggc tgtcgccatg attctggaga
    12301 acggcggcgc gggtccggcg gttggtacgc tgatgcgcca gatcctcgac cacattatgc
    12361 tgggtgataa caacaccgat ctgcctgcgg aaaatccagc ggttgccgca gcggaggacc
    12421 attaatcatg acggataatc cgaataaaaa aacattctgg gataaagtcc atctcgatcc
    12481 cacaatgctg ctgattttac tggcattgct ggtttacagc gccctggtta tctggagcgc
    12541 cagcggtcag gatattggca tgatggagcg taaaatcggc caaatcgcga tgggtctggt
    12601 catcatggtg gtgatggcgc aaattcctcc acgcgtttat gaaggctggg ccccctatct
    12661 ctatatcatc tgtattattt tgctggtggc ggtagatgct ttcggtgcca tctctaaagg
    12721 cgctcaacgc tggctggacc tcggtattgt tcgttttcag ccgtcggaaa ttgccaaaat
    12781 agccgtacca ctgatggttg cgcgctttat caaccgcgac gtttgcccgc catcgttgaa
    12841 gaacactggc atcgcgctgg tgctgatatt tatgcccacg ctgctggtgg ctgcacagcc
    12901 tgacctggga acatcaatcc tcgttgcgct ttccggtctg tttgtactgt tcctctctgg
    12961 ccttagctgg cgtctgattg gcgtcgcagt agtgttggtg gcggcgttca ttccgattct
    13021 gtggttcttc ctgatgcatg attaccagcg ccagcgcgta atgatgctcc tggacccgga
    13081 atcagaccca ctcggcgcgg gctatcacat tattcagtct aaaattgcta ttggctccgg
    13141 tggattacgc ggcaaaggct ggctgcacgg cactcagtca cagcttgaat ttctccccga
    13201 acgccatacc gactttatct tcgcggtact ggcggaagag ctgggattag tgggcatttt
    13261 gattctgctc gctctctaca ttctgctgat catgcgcggg ctgtggatag ccgccagagc
    13321 gcaaaccacc tttggtcgcg tcatggctgg cggcttaatg ctgatattat tcgtttatgt
    13381 cttcgtaaat attggtatgg taagcggtat tctgccggtt gtaggggttc cgctcccact
    13441 ggtcagttat ggaggatcgg cgctaattgt gctgatggct gggttcggga ttgtaatgtc
    13501 aatccacacc cacaggaaaa tgttgtcgaa aagcgtgtaa gaggtgcgca atgcgtaagc
    13561 agtggctcgg gatctgcatc gcggcaggaa tgctcgcggc atgtacaagc gatgatggtc
    13621 agcaacagac ggtaagtgta ccgcagcctg cggtatgtaa cggccccata gttgaaatta
    13681 gcggggcgga cccgcgtttc gaaccactga acgcgacggc aaatcaggat taccagcgcg
    13741 acggtaaaag ctacaaaatc gtgcaggatc cgtctcgatt tagccaggcg ggactggcgg
    13801 caatctatga tgccgaacca ggcagtaacc tgacggcctc tggcgaagct ttcgatccga
    13861 cacagctgac ggcggcccat ccaacgcttc cgatccccag ctacgccaga atcactaacc
    13921 tggctaacgg gcgaatgatc gtggtgcgca ttaatgatcg cggtccttac ggcaacgacc
    13981 gcgttatttc gctttctcgc gcagcagctg accgtcttaa cacgtcaaac aacaccaaag
    14041 ttcgtatcga tccgattatt gtcgcccagg atggttcgct ttctggtcct ggtatggctt
    14101 gtaccacagt cgccaaacag acttacgccc tgcctgcacc tcccgattta agcggtggcg
    14161 cgggaacaag ttcagtgtct ggcccgcagg gtgacattct tccggtcagt aattcgacgc
    14221 taaaaagcga agatccgacc ggcgcgccgg taaccagcag cggtttcctc ggcgcaccaa
    14281 cgaccttagc gcctggtgta ctggaaggca gcgaaccgac gcctgcgcca cagcccgttg
    14341 ttacagctcc gtcgacaacg cctgcaacct cgcctgcaat ggtgacaccg caagccgcct
    14401 cgcaaagcgc cagcggcaac tttatggtgc aggtcggggc cgttagcgat caggctcgtg
    14461 cgcaacagta ccaacagcaa ctgggacaga agttcggcgt ccccggtcgc gtaactcaaa
    14521 atggcgcagt ctggcggatc cagcttggcc cattcgccag caaagccgaa gccagtacct
    14581 tgcagcaacg tttgcaaacc gaagcccaat tacagtcatt tattaccacc gcgcagtagc
    14641 gtaaagcagg catctgatgt gtcaatttct gtaaatgacg ttaacaaagt cacgcggaaa
    14701 gtcagatgcc tgccggtagt gcatttgcta tagtagggca ctttttttaa ttccatcacg
    14761 gatgtcgtag ttcagaccat gaataccatt ttttccgctc gtatcatgaa gcgcctggcg
    14821 ctcaccacgg ctctttgcac agcctttatc tctgctgcac atgccgatga cctgaatatc
    14881 aaaactatga tcccgggtgt accgcagatc gatgcggagt cctacatcct gattgactat
    14941 aactccggca aagtgctcgc cgaacagaac gcagatgtcc gccgcgatcc tgccagcctg
    15001 accaaaatga tgaccagtta cgttatcggc caggcaatga aagccggtaa atttaaagaa
    15061 actgatttag tcactatcgg caacgacgca tgggccaccg gtaacccggt gtttaaaggt
    15121 tcttcgctga tgttcctcaa accgggcatg caggttccgg tttctcagct gatccgcggt
    15181 attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg ccgattttgc cgctggtagc
    15241 caggatgctt ttgttggctt gatgaacagc tacgttaacg ccctgggcct gaaaaatacc
    15301 cacttccaga cggtacatgg tctggatgct gatggtcagt acagctccgc gcgcgatatg
    15361 gcgctgatcg gccaggcgtt gatccgtgac gtaccgaatg aatactcgat ctataaagaa
    15421 aaagaattta cgtttaacgg tattcgccag ctgaaccgta acggcctgtt atgggataac
    15481 agcctgaatg tcgacggcat caaaaccgga cacactgaca aagcaggtta caaccttgtt
    15541 gcttctgcga ctgaaggcca gatgcgcttg atttctgcgg tgatgggcgg acgtactttt
    15601 aaaggccgtg aagccgaaag taaaaaactg ctaacctggg gcttccgttt cttcgaaacc
    15661 gttaacccac tgaaagtagg taaagagttc gcctctgaac cggtttggtt tggtgattct
    15721 gatcgcgctt cgttaggggt tgataaagac gtgtacctga ccattccgcg tggccgcatg
    15781 aaagatctga aagccagcta tgtgctgaac agcagtgaat tgcatgcgcc gctgcaaaag
    15841 aatcaggtcg tcggtactat caacttccag cttgatggca aaacgatcga acaacgcccg
    15901 ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg gcaaaatcat tgattacatt
    15961 aaattaatgt tccatcactg gtttggttaa aaatcgaaca cttgaaagtg taatttccgt
    16021 ccccatatac taagcatcag taaaaaactc ccgccttctg gcgggagttg ctatttaatt
    16081 acgttacgcc ggagctgaca tgaaaaccaa acttaacgaa ctgcttgaat tccctactcc
    16141 ttttacttac aaagttatgg ggcaggcgtt acctgagctg gttgatcagg tggttgaagt
    16201 ggtacagcgc catgcgccag gtgactacac cccaacggta aaaccaagca gcaaaggcaa
    16261 ctaccactcg gtatctatca ctatcaacgc cactcatatc gagcaggttg aaacactgta
    16321 tgaagaactg ggcaaaatcg atattgtccg catggttctg taactcgctt ctccgttacc
    16381 cggcacctgg tcgggtaact cccctctcct cgctgtgata tacttttccc ccacttttac
    16441 tcattctcca cggagatgcc gttttgtatc aggataaaat tcttgtccgc cagctcggtc
    16501 ttcagcctta cgagccaatc tcccaggcta tgcatgaatt caccgatacc cgcgatgata
    16561 gtacccttga tgaaatctgg ctggtcgagc actatccggt attcacccaa gggcaggcag
    16621 gaaaggcgga gcacatttta atgcctggtg atattccggt gattcagagc gatcgcggtg
    16681 ggcaggtgac ttatcacggg ccggggcaac aggtgatgta tgtattgctt aacctgaaac
    16741 gccgtaaact cggtgtgcgt gaactggtga ccttgcttga gcaaacagtg gtgaataccc
    16801 tggctgaact gggtatagaa gcgcatcctc gggctgacgc gccaggtgtc tatgttgggg
    16861 aaaagaaaat ttgctcactg ggtttacgta ttcgacgcgg ttgttcattc cacggtctgg
    16921 cattaaacgt caatatggat ctttcaccat ttttacgtat taatccttgt gggtatgccg
    16981 gaatggaaat ggctaaaata tcacaatgga aacccgaagc gacgactaat aatattgcac
    17041 cacgtttact ggaaaatatt ttagcgctac taaacaatcc tgacttcgaa tatattaccg
    17101 cttaatttca tacctcaatg gcccaattta cactgggtca ttactcataa cacctgctaa
    17161 tgctttacat ctccttcgcg tttgtatatt ttctttatca cttgcagaaa tgcgatgcta
    17221 cgccttgctc cgtaactcac cccattgatt aacttattga aatagttgat tatgatgagc
    17281 aaaagcgttc cgttccaatt agacataaga ttattgtaat ttttttaaat attatttttc
    17341 catgaataaa attggagtaa gtgcgtggat agtaataatc aaatcgaacc ctgtttaagc
    17401 agaaagtcgt cagaaggtaa accacaaata tttacaacct tacgaaatat tgatcttaac
    17461 cttctgacta tttttgaagc tgtgtatgta cataaaggga tcgttaatgc agcgaaagtg
    17521 cttaatctga ccccctcggc aatcagtcag tctatacaga aactgcgcgt tatattccct
    17581 gacccattgt ttattcgcaa aggccagggt gtcactccta ccgcatttgc gatgcatcta
    17641 catgagtata tcagtcaggg ccttgaatcc attcttggcg cgctggatat cgaaggaagc
    17701 tatgataagc aacgaacgat aactatcgcg accaccccct cggtcggagc cctggtcctt
    17761 cctgtcatct atcgggcgat taaaactcac tacccgcagc ttttactgcg caacccgccc
    17821 atcagcgacg cggaaaacca actcagtcag tttcaaaccg atctcatcat cgataacatg
    17881 ttttgcacca atcgtacggt gcaacatcat gttctgttca ccgacaatat ggtgttaatt
    17941 tgccgcgagg gaaatccact actctcttta gaagatgaca gagagactat cgacaacgct
    18001 gcgcatgtac tcctgttacc agaagggcaa aatttcagcg gtctgcggca gagagttcaa
    18061 gagatgtttc cggaccggca aatcagcttc accagctaca atattttgac aatcgctgca
    18121 ctggttgcca acagtgacat gttagcgatt attcccagcc gtttttataa cttgtttagc
    18181 cgttgctggc cgctggaaaa actgcctttt ccgtccttaa atgaggagca aattgatttc
    18241 tctatccact acaacaaatt cagcctgcgt gatcccatcc tgcacggggt aatagatgtt
    18301 atccgtaatg cattttgaaa agtattgtta cagccagatc acagcgcaga acgataaaat
    18361 ggcacaatcc aacaacaatt ttacattttc gcgaccgctt tggctgcttt caggtccgtt
    18421 tcaatgatat actgccagtc gttaattcaa aaatagttga taattacaac aatctattga
    18481 attgaaacgc tttccttcgt aattcgcaac tggaacacgc acgctatgag taaacccatt
    18541 gtgatggaac gcggtgttaa ataccgcgat gccgataaga tggcccttat cccggttaaa
    18601 aacgtggcaa cagagcgcga agccctgctg cgcaagccgg aatggatgaa aatcaagctt
    18661 ccagcggact ctacacgtat ccagggcatc aaagccgcaa tgcgcaaaaa tggcctgcat
    18721 tctgtctgcg aggaagcctc ctgccctaac ctggcggaat gcttcaacca cggcacagca
    18781 acgtttatga tcctcggcgc tatttgtacc cgccgttgtc cgttctgtga cgttgcccac
    18841 ggtcgcccgg tagctcctga tgccaatgag ccagtgaaac tggcgcagac cattgccgat
    18901 atggcgctgc gttatgtggt tatcacctcc gttgaccgtg atgacctgcg cgatggcggt
    18961 gcccaacact ttgcggattg tattactgcc attcgggaaa aaagcccgca aatcaaaatt
    19021 gaaactctgg tgccggattt ccgcggtcgt atggatcgtg ctctggatat tctgactgca
    19081 acgcccccag atgtgttcaa ccataacctg gaaaacgtac cgcgtattta ccggcaggta
    19141 cggcctggcg cagattacaa ctggtcgctg aagctgctgg aacgctttaa agaagcgcat
    19201 ccggaaatcc cgaccaagtc tggtctgatg gtgggtctgg gtgaaaccaa tgaagaaatt
    19261 attgaggtaa tgcgcgacct gcgccgtcat ggtgtgacga tgttaacgct ggggcaatat
    19321 ttgcagccaa gccgccatca cctgccggtt caacgttacg ttagcccgga tgagttcgac
    19381 gaaatgaaag ccgaagcgct ggcgatgggc tttacccatg ctgcatgcgg tccgtttgtc
    19441 cgctcttctt accacgccga tttgcaggcg aaagggatgg aagttaagta atcctgtaat
    19501 aattacttac atctgtctga taaaaaaccc gctttttgaa gcgggttttt tgtatcgaac
    19561 aagatattga gggagcgtcc tgctcgccac gtcactcttt atgagagagc ttttcagcct
    19621 gaagatcaac gtctgcgcct tttttcgccg cagcatcgtc atcattcatc gccttcttga
    19681 accctttaat ggccgctcca aggtctccgc ccagcgtacg taacttctta gtcccaaaca
    19741 gcagaacgac cagcgccgca actaccagca gtttggtaat actaatctca cccatagata
    19801 ccttcttgac atataaccgg caacagaacg cgccttaaaa accagagaat attaacggtc
    19861 attcgacgcg cgcacacaat agcaatctgt aacaaggtga atcaagcgcc gagtaaaaaa
    19921 acatcataat aattgcggcg gcgcaaagcg acggttgttt aagacgggca gttgcgcgcg
    19981 cacctgacgc acacgttcgg gcgtcacttc cgccataatg agtgcaggca tttctgacgc
    20041 tgccgcaatg gtgacgccaa agggatctat aatccggctt tgcccgatat ttttgttccc
    20101 gcactccccc gccgccacca tataacaggt ggtatccagc gcacgagcgg caagcaacgt
    20161 tgaccagtga tgctctttga gcggcccgcg aacccaggcg gcaggaagta ccaggatttc
    20221 agctccctgt aatgcctgtg ccagcgccag ctctggaaag cgtaagtcat aacaggtcat
    20281 cagaccgacc ttcatccctt ccacctccag taacggagcg atttcattac cagcatcaac
    20341 acggcgtgat tcctgaatgg caaatgcatc atagagatgc agtttggcat aacgggcgac
    20401 gatgtttcct gcctgtagcg ccaccagcat attccatgcg cgccccggcg ttgaaggaac
    20461 atgaatcgtc agaattgtcg tcatcatgtt acgtttactt tctcgccgta aacgtccgag
    20521 gaattcgcct tccagcagct gtgctgattt aaccgataga tctgcatcat gatcatcgcg
    20581 tgccagcaat gcttccggca ggacaaacag cgatacgtcg ttttccgccg cctgcgccat
    20641 caacgaggca caaatctcag cgttcttttc ccacacagat gtaacagcaa actgtcctgc
    20701 agcagccagc attgccacct cctgtcagag tcgtaaaatt cgccaacgac gattattcgg
    20761 cgttacactt atcactcata caaatcaaat agcaggattt tgcagtgtta caacttcttt
    20821 tagcagtttt tattggcggt ggtacaggaa gcgtggcgag atggctgtta agtatgcgat
    20881 ttaacccgct gcatcaggcg attccgttgg ggacgctggc agcaaacctg attggggcat
    20941 tcatcatagg aatgggattc gcctggttca gcaggatgac gaacattgat ccagtgtgga
    21001 aagtattaat caccaccgga ttttgtggcg gtcttacaac cttctcaaca ttttcggcag
    21061 aagtggtgtt tttgttacaa gagggccgct ttggctgggc gttactgaac gttttcgtca
    21121 accttctggg gtcttttgcc atgaccgcac tggcattctg gctgttttcg gcctcaaccg
    21181 cacactaaag accaaaaaaa acccgctgat taagcgggtt ttgaattctt gctgacgtat
    21241 cttacagagc gattacgttt gcagcagaag ggcctttggc accgttagtg atttcgaact
    21301 ctacgcgctg accttcagca agagttttaa aaccattagt ctggattgca gagaagtgta
    21361 cgaacacgtc tttgctgccg tcttccggag taatgaaacc gaatcctttg gactcattaa
    21421 accacttaac gttaccttta atcttagaca tcaaaattac ctttacatga aaaatagaca
    21481 caaatgctgt gtcggttacc agtacaccaa ttgtggtacg ctttgtccag tgcaacttta
    21541 ctaaaaagtg ataaaagtcg cgttattttt gatgttaatt attttagtaa ttgattttaa
    21601 agaatttact aaaacttcat ttgtctcaaa actgaaagcg catccaggca aagtacacat
    21661 tgccattgtt gtaggtaccc ggaatatagg tcatctgaaa agtcactgga ccataaccca
    21721 cggaggccaa tggcagtaga accgggagag ggatgtaatt ccagttatca cgtgccgtta
    21781 cgccagcggt gaatcccaga cctaaatgaa aattttcatc cgccagcggt cgccaggtac
    21841 tttcccatcc gtatccggca atcggttccc atttgttcca cgagtcctta aatgccatgg
    21901 catacaggcc atgccagttt cctttttcat cccaacgcga caggccaaaa ccgccacccc
    21961 acggtcgctc gttatagcga tcggtttttt ctttgtcgta agcgaaacgt gcatgccagg
    22021 tgatggcagg aatatataaa tcataatgtt caggctgttg ccaggtttgt gcaatatttt
    22081 ctctaaacgt tgtcatccac tcatctgcgt tagcaaaagc tttaccaacg ctgattaact
    22141 gaataaaaac aaaggaaaag atagcgacat atttactcac gttcatttgt gaccataaaa
    22201 catttatcaa aaatctacta ctagcatagc aaagctacaa ttaacataac cttaatagcc
    22261 ccaacataaa gaataatctg aatagctgct ctatttattt ccgcttgttc aagttaaata
    22321 tttccgcgca tcgattaaag atgattcgtc gttttctgcg atgggaatag tcataaaaga
    22381 aaaagcaagt tgtttctgat aacaaaatgc aaccaagaga aaattacttt attttttaac
    22441 agcatacaaa aagcatcata acgaagaata tattatatat cgtcaaccca cgcccattgc
    22501 agattatttt ttgagcacac aaccgcagct aacaccattg caattaacaa atttgcatca
    22561 atccaccatc aatttacaca cattattatg cgataattgc caaacgctaa atatgcgttt
    22621 tgttatctat gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta
    22681 atcagca
//
LOCUS       DAZFFG010000079        22274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFFG010000079 DAZFFG010000000
VERSION     DAZFFG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.079"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..222
                     /locus_tag="ACHJ7J_003300"
     CDS             <1..222
                     /locus_tag="ACHJ7J_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5001491.1"
                     /translation="TSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAA
                     GHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(262..1476)
                     /gene="ydcO"
                     /locus_tag="ACHJ7J_003301"
     CDS             complement(262..1476)
                     /gene="ydcO"
                     /locus_tag="ACHJ7J_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO5001492.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLGGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQNPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1529..2065
                     /gene="sutR"
                     /locus_tag="ACHJ7J_003302"
     CDS             1529..2065
                     /gene="sutR"
                     /locus_tag="ACHJ7J_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO5001493.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVEGEWQSLNCGEGARFAADVTHIYR
                     NGGEQIVHFHSLIHYPRS"
     gene            2138..4099
                     /gene="rlhA"
                     /locus_tag="ACHJ7J_003303"
     CDS             2138..4099
                     /gene="rlhA"
                     /locus_tag="ACHJ7J_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO5001494.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTDPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4191..4421)
                     /gene="yncJ"
                     /locus_tag="ACHJ7J_003304"
     CDS             complement(4191..4421)
                     /gene="yncJ"
                     /locus_tag="ACHJ7J_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO5001495.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLCHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4643..4819
                     /gene="hicA"
                     /locus_tag="ACHJ7J_003305"
     CDS             4643..4819
                     /gene="hicA"
                     /locus_tag="ACHJ7J_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO5001496.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4865..5281
                     /gene="hicB"
                     /locus_tag="ACHJ7J_003306"
     CDS             4865..5281
                     /gene="hicB"
                     /locus_tag="ACHJ7J_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO5001497.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5360..6766
                     /gene="ydcR"
                     /locus_tag="ACHJ7J_003307"
     CDS             5360..6766
                     /gene="ydcR"
                     /locus_tag="ACHJ7J_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5001498.1"
                     /translation="MKKYQQLAEQLREQIVTGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLMPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGTMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7011..8156
                     /gene="ydcS"
                     /locus_tag="ACHJ7J_003308"
     CDS             7011..8156
                     /gene="ydcS"
                     /locus_tag="ACHJ7J_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO5001499.1"
                     /translation="MSKTIARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8174..9187
                     /gene="ydcT"
                     /locus_tag="ACHJ7J_003309"
     CDS             8174..9187
                     /gene="ydcT"
                     /locus_tag="ACHJ7J_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5001500.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAENLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9188..10129
                     /gene="ydcU"
                     /locus_tag="ACHJ7J_003310"
     CDS             9188..10129
                     /gene="ydcU"
                     /locus_tag="ACHJ7J_003310"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5001501.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10119..10913
                     /gene="ydcV"
                     /locus_tag="ACHJ7J_003311"
     CDS             10119..10913
                     /gene="ydcV"
                     /locus_tag="ACHJ7J_003311"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5001502.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10935..12359
                     /gene="patD"
                     /locus_tag="ACHJ7J_003312"
     CDS             10935..12359
                     /gene="patD"
                     /locus_tag="ACHJ7J_003312"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO5001503.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12456..12551)
                     /gene="yncL"
                     /locus_tag="ACHJ7J_003313"
     CDS             complement(12456..12551)
                     /gene="yncL"
                     /locus_tag="ACHJ7J_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO5001504.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12746..12919
                     /gene="ortT"
                     /locus_tag="ACHJ7J_003314"
     CDS             12746..12919
                     /gene="ortT"
                     /locus_tag="ACHJ7J_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO5001505.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            13005..13238
                     /gene="ydcY"
                     /locus_tag="ACHJ7J_003315"
     CDS             13005..13238
                     /gene="ydcY"
                     /locus_tag="ACHJ7J_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO5001506.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13239..13688)
                     /gene="ydcZ"
                     /locus_tag="ACHJ7J_003316"
     CDS             complement(13239..13688)
                     /gene="ydcZ"
                     /locus_tag="ACHJ7J_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5001507.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13685..14203)
                     /gene="mddA"
                     /locus_tag="ACHJ7J_003317"
     CDS             complement(13685..14203)
                     /gene="mddA"
                     /locus_tag="ACHJ7J_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO5001508.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14384..15421
                     /gene="curA"
                     /locus_tag="ACHJ7J_003318"
     CDS             14384..15421
                     /gene="curA"
                     /locus_tag="ACHJ7J_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO5001509.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVKSNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15619..16284
                     /gene="mcbR"
                     /locus_tag="ACHJ7J_003319"
     CDS             15619..16284
                     /gene="mcbR"
                     /locus_tag="ACHJ7J_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO5001510.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16320..18422)
                     /gene="pqqU"
                     /locus_tag="ACHJ7J_003320"
     CDS             complement(16320..18422)
                     /gene="pqqU"
                     /locus_tag="ACHJ7J_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO5001511.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            18664..19725
                     /gene="yncE"
                     /locus_tag="ACHJ7J_003321"
     CDS             18664..19725
                     /gene="yncE"
                     /locus_tag="ACHJ7J_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO5001512.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(19838..21337)
                     /gene="ansP"
                     /locus_tag="ACHJ7J_003322"
     CDS             complement(19838..21337)
                     /gene="ansP"
                     /locus_tag="ACHJ7J_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO5001513.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVYLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKAGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21604..22221
                     /gene="yncG"
                     /locus_tag="ACHJ7J_003323"
     CDS             21604..22221
                     /gene="yncG"
                     /locus_tag="ACHJ7J_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO5001514.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNVII"
BASE COUNT         5657 a         5491 c         5761 g         5365 t
ORIGIN      
        1 acaagccagc gttgcgcgta ctgtggtcat acagcgaaag agaaccgcct gtcacaaagt
       61 aaattcagat gccaggtatg tggatataca gcgaacgccg atgtaaatgg cgctcgtaac
      121 attttagcgg cggggcacgc cgttcttgcc tgtggagaga tggtgcagtc aggccgcccg
      181 ttgaagcagg aacccaccga aatgattcag gcgacagcct gaacgtagca gggatccacg
      241 tccttcaggg cgtggaggat gtcaatatcg gtttctgtca gcgattaaat tcaacaccac
      301 gtaacaaaca cctccggcaa ttaatcccca aaacgcagaa ccaatcccga ccagcgtcaa
      361 tccacttgcc gttaccagaa atgccaccac cgccgcgtcg cgctcacgct cattatgcag
      421 cgcctgatac aaactgccgc caatggtact taacagcgcc agacctgcca gcatctggat
      481 ccaacttacg ggcagggcag ccatcatccc ggtaatggca ctaccaaaca gacctgcgag
      541 caaatagaaa atgcctgcaa cggcagctgc cagccaacgt tgatctttat ccggatgcgc
      601 ttccgggttt tggcaaatag ccgcggtgat tgccgcaata ccgactgaat aaacgccgaa
      661 aggggaaaaa aacagtgcca gcaatccagt aaatacaatt aatggcgaaa caggagttga
      721 atatccggcg gctttcattg ctgcgatacc cggtgcgttt tgcgatgcca tcgtcaccag
      781 aaaaaggggg agtgcaacgc tcaggctgtg agcaaacgaa aaatcagggg taatataagt
      841 ggggagaacg ggtttaaaga caacatcagt tgtgacaacg tcaccttgcg cgataacgat
      901 cacgatccca ataatcatcg cggcaattac cgcatagcgc ggcgcaacgg ccttggttgc
      961 cagccatacc agcaacatac ttccacacaa cgtaaattga ccgcccagac tggcaaacgc
     1021 ctgtaaacca aagcgtaata aaatcccggc aagcattgcc gccgcaagcg agtgcggaat
     1081 aatgcgcatc agacgagcaa agagtcccgt tatgccgcag aggactatta acgcgttggt
     1141 gacaataaaa acgccgatgg cttcgttaag tgttagtccc tgcaatccgg tgaccaacaa
     1201 agccgcgcca ggcgttgacc atgcggtgag aacaggtacg cgataccata atgtcagagt
     1261 cagcgtactg acgcccattg ccagccccag cgccgtcatc cagccagaga tttgtgcagt
     1321 ggtggctccg gcgacaatcg ctgcttgcca gattattgcc gctgaactgg cgtagccaat
     1381 taataccgcc agaaaccccg ccagtagcgt gggtggagga atagaaaaca gacgcatagt
     1441 cacctcgtgc gttatagcgt acaggcaccg tagcatttgt ccgttataac gcacaagtga
     1501 taaacttccg ttttgccgga ggagtcgcat ggaaaatctc gctcgctttt tatccaccac
     1561 acttaaacaa ctacgtcagc agcgcggctg gagtctttcg cgactggcag aagcgacggg
     1621 cgtttctaaa gcaatgttag gacagattga gcgtaatgag tccagcccga cggtcgcgac
     1681 attatggaaa attgccactg ggttgaacgt gccattttcg acatttattt ctccgccaca
     1741 gtccgctacg ccttcagttt atgatccaca acagcaggcg atggtgatca tttcgctgtt
     1801 tccttatgat ccacaactct gctttgaaca cttctcgatt cagatggcac ccggtgcaat
     1861 aagcgaatcg acgccccatg agaagggggt aatcgaacat gtcgtcgtca ttgatggaca
     1921 acttgatctg tgcgttgagg gtgaatggca gagccttaat tgtggggaag gcgctcgatt
     1981 tgctgcagac gtcacgcata tctatcgtaa tggtggggag caaatcgtac attttcattc
     2041 cctcatccat tacccgcgca gttaagcggg aaaactattt cgcaacgtcg cgcttgtggc
     2101 taaaatagcc gccatttttc agctactgga taagaatgtg accgtatctt ctcatcgact
     2161 tgaactgtta agcccggcac gcgatgccgc cattgcccgc gaagctattt tgcacggtgc
     2221 tgatgctgtt tatatcggcg gccctggttt tggtgcccgt cataatgcca gtaatagcct
     2281 gaaagatatt gccgagctgg tgccgtttgc ccatcgttat ggtgcaaaaa ttttcgtcac
     2341 gcttaacacc attttgcatg atgatgagct ggaacccgcg caacggctga ttactgacct
     2401 ctaccagacc ggtgtcgatg cgctgattgt tcaggatatg gggattctgg aacttgatat
     2461 tccgccgatt gaactgcacg ccagtacgca gtgcgacatt cgtacagtag aaaaagcgaa
     2521 gttcctctct gatgttggct tcacgcagat tgtgctggcg cgagagctga atcttgatca
     2581 gatccgcgcg attcaccagg ctacggacgc gaccattgaa ttctttattc atggggcact
     2641 gtgcgtggcc tattcgggtc agtgctacat ttctcatgcg caaacagggc gtagcgccaa
     2701 ccgtggcgat tgctcgcagg cgtgccgttt gccatacaca ttgaaagacg atcaggggcg
     2761 ggtggtttcc tatgaaaaac atctgctgtc gatgaaagat aacgatcaga ctgccaacct
     2821 cggcgcgctg attgatgctg gcgtacgctc cttcaagatt gaagggcgtt acaaagatat
     2881 gagctacgtg aagaatatca ccgcccatta tcgccagatg ctggatgcca ttattgaaga
     2941 acgtggcgat ctgacgcgtg cttcatcagg tcgtactgaa catttctttg ttccatcgac
     3001 ggaaaagaca ttccaccgtg gtagcacaga ttattttgta aatgcccgta aaggcgatat
     3061 tggcgcgttc gattcgccga aatttatcgg cctgccggtg ggtgaagtat tgaaagtggc
     3121 gaaagatcat ctcgatgttg ccgttaccga tccactggca aatggcgatg gcctgaacgt
     3181 gttgattaaa cgtgaagtcg tcggttttcg tgccaatacg gtcgagaaaa ccggagaaaa
     3241 tcagtaccgc gtctggccca atgaaatgcc agcagatttg cacaaaattc gcccgcatca
     3301 cccactaaac cgtaatcttg atcataactg gcagcaggca ctgacaaaaa cctccagcga
     3361 acgtcgggtg gcggtagaca ttgaactggg cggttggcag gaacaattga ttctgaccct
     3421 caccagtgaa gagggggtca gcatcacgca tacgctggat gggcagttcg acgaagccaa
     3481 taacgccgaa aaagcaatga acaatctgaa ggatggtctg gcaaaactgg ggcaaaccat
     3541 ctattacgcc cgcgatgtgc aaattaattt gccgggggcg ctgtttgtac caaacagtct
     3601 gttaaaccag ttccgccgtg aagctgctga catgctggat gctgcgcgtc ttgccagtta
     3661 ccagcgcggc agccgtaaac cggttgctga tcctgcgccg gtttatccgc aaacgcatct
     3721 gagtttcctc gcgaacgttt acaaccagaa agcgcgtgaa ttttatcatc gctatggtgt
     3781 gcagctgatt gacgcggcgt atgaggcaca tgaagagaag ggcgaagtcc cggtgatgat
     3841 caccaagcat tgtctgcgct ttgcctttaa tctgtgcccg aaacaagcga aaggcaatat
     3901 caaaagctgg aaggctacgc caatgcaact ggttaacggc gatgaagtat taacgctaaa
     3961 gtttgattgc cgcccatgcg agatgcacgt tattggcaaa atcaaaaatc acatactgaa
     4021 aatgccgtta ccgggaagcg tagtggcatc cgtaagtccg gatgagctgc tgaaaacatt
     4081 gccgaagcga aaagggtaaa acgccagttt tctggttact caccactcat tgaatctgca
     4141 tgatattgcc tgccgggtaa ggcgtcacgc cgcatccggc atcaaacgac tcaacgttgt
     4201 cccgcttctg gtttgcgcga tttttgccag taaaaatgct cgcgcaaacc ttccgccgac
     4261 tcttctgcca cagcacgcaa ttcatcgctg tccgcttcat gacacagctg atgatccaca
     4321 ttctttaccc acacaaattc atgtcctttg tgccctgcca tgagttgtcc tgagaacagc
     4381 gcacacgtta ataagacaac cgataacgcc ttcgtaaaca tcctgccacc tttttcttac
     4441 cttttgccgc tatgatgccg atcgtttctt gaggttatta ttcagttttg caaattagcg
     4501 caaagaaatt ctggaatctt ccttcctgat tttgcattgc attctgccgt tgcggcgatt
     4561 tagtgctatt ttcgagcaaa ttacacacgg aggtaaacac taatgtttac tttgttgata
     4621 tactcagcgg cagggaggcg atgtgaaaca aagcgagttc agacgttggc tcgaatctca
     4681 gggcgtcgat gtagcgaatg gcagcaacca tttgaaactc aggtttcatg ggaggcgcag
     4741 tgtcatgccg cgtcacccct gcgatgagat taaagaacca ttgcgtaaag caatcctgaa
     4801 acaactcggt ttgagttaat cgccaattaa aaaggttaat gacatgcgag agacagtcga
     4861 aattatgcgt tatcccgtca ctcttacacc cgcgccggaa ggcggttata tggtttcttt
     4921 tgtggatatc cctgaagcgt tgacccaggg cgaaactgtc gctgaagcga tggaagcggc
     4981 aaaagatgct ttactgaccg catttgattt ttattttgaa gataacgagc ttatcccgtt
     5041 accttcgcca ttaaatagtc acgatcattt tattgaagta cctttgagcg tcgcctctaa
     5101 ggtattgctg ttaaatgctt ttttacagtc agaaatcact cagcaagagt tagccaggcg
     5161 aattggcaaa cctaaacagg agattactcg cctatttaac ttgcatcatg cgacaaaaat
     5221 cgacgccgtc cagctcgcgg caaaggcgct tggcaaagag ttatcgctgg tgatggttta
     5281 attacagtta atgaaaagtt gtcattttta acaactgata tagactgccg aatcatctgc
     5341 acataattac gattcgataa tgaaaaaata ccagcagctt gcagaacaat tacgcgagca
     5401 gattgtgacg ggtatctggc aacccggcga tcgtttgcct tcgttgcgtg accaggtggc
     5461 gctttctggc atgagcttta tgactgtcag ccatgcctat cagttgctcg aaagtcaggg
     5521 atatattatc gcacgaccgc agtcgggtta ttacgttgcg ccacaggcaa taaaaatgcc
     5581 gaaagcgcca gtcattccag tcactcgaga tgaagcagtc gatatcaaca cttatatttt
     5641 tgatatgttg caggccagtc gcgatccgtc ggtcgttccg tttgcctcgg cctttcccga
     5701 cccgcgactt ttccccctcc aacaactaaa ccgctcgctg gcgcaggtaa gcaaaaccgc
     5761 cacggcgatg agcgtgattg aaaacttgcc gccaggaaac gcagaactgc gccaggctat
     5821 tgcccgtcgc tatgccttac agggcatcac catttctcct gatgaaattg tcattactgc
     5881 cggggcgtta gaggcattaa acctcagttt gcaagcggta actgaaccgg gcgattgggt
     5941 gatagtagag aatccttgtt tctacggtgc gttgcaggcg ctggagcggc tacggctgaa
     6001 ggcgttatcg gtggcgacgg atgttaaaga agggatcgat cttcaggcgc tggaactggc
     6061 gttgcaggag tatccggtga aagcgtgctg gctgatgact aatagccaga atccactcgg
     6121 atttacctta atgccgcaaa aaaaagcaca actggtggcg ttgctcaatc agtacaacgt
     6181 aacgctgatt gaagatgacg tttacagcga actttatttt ggacgggaaa aaccgctgcc
     6241 tgcgaaagcg tgggatcgcc acgatggcgt tttgcattgc tcttcgtttt cgaaatgtct
     6301 ggtgcctggt tttcgtattg gttgggtcgc cgccggaaaa catgcacgta aaattcaacg
     6361 cttgcagttg atgagtacgc tttccaccag ctcaccgatg cagcttgcgc tggtggatta
     6421 cctttccacg cgccgatacg acgcccatct tcgtcgcctg cgtcgccagc ttgcggaacg
     6481 taaacaacgt gcctggcagg cactgctgcg ttatctgcct gcggaagtga aaattcatca
     6541 taatgacagc ggttattttc tctggctgga gctccccgag ccgttagatg ccggcgaatt
     6601 gagcctggcg gcactgacgc atcatatcag tattgcgccg ggtacaatgt tttctaccgg
     6661 tgaaaactgg tcacgttttt ttcgttttaa taccgcgtgg cagtggggag aacgtgaaga
     6721 acaggcggta aaacaattag gcaaacttat tcaagaacgg ctgtaatagc gtttaattta
     6781 attcctctta gattgggtaa tatgaatttc gaatagcagt catatttcct aactccttga
     6841 ctatactcca gaagataacc ttacagacgg cataatgcgc ggtagctcac aacctgatta
     6901 aattttctca ggggcgaagg tgtgcctgca agccgccgtc tatggttaaa caaggagata
     6961 tttttacggc acggcggctg aacaattaat tacgacagga gtaagacctt atgagcaaga
     7021 caattgcccg cagcagcctg tgtgcgctca gcatgacaat aatgaccgct cacgccgccg
     7081 aaccgcctac caatttagat aaaccggaag ggcgactgga tattatcgcc tggccgggat
     7141 atatcgaacg cggacaaact gataaacaat acgactgggt aacgcaattc gaaaaagaga
     7201 caggctgcgc ggtgaatgtt aaaaccgccg cgacttccga tgaaatggtc agtctgatga
     7261 ccaaaggggg ttacgatctg gttacggcat ccggcgatgc ctcgctgcgt ttgattatgg
     7321 gtaaacgcgt gcagccgatt aataccgcct tgattcccaa ctggaaaacg cttgatccgc
     7381 gcgtggttaa aggcgactgg tttaacgttg gcggcaaagt ttacggcaca ccttaccaat
     7441 gggggccgaa cctgctgatg tacaacacta aaaccttccc gacgccgccg gatagctggc
     7501 aagtggtttt tgttgagcaa aatctgcagg acggcaagag caataaaggc cgcgttcagg
     7561 cttatgatgg ccctatctac attgcggacg ctgcgttgtt cgttaaagcc actcagccgc
     7621 agttgggcat cagcgatccg tatcaactca ccgaagaaca gtatcaggcg gtgctgaaag
     7681 tgctgcgcga tcaacatagt ttgatccatc gctactggca tgacactacc gtgcaaatga
     7741 gcgatttcaa aaacgagggt gtggttgctt ccagtgcatg gccctatcag gccaacgcct
     7801 tgaaagccga aggccagcct gttgctaccg ttttcccgaa ggagggtgtt accggttggg
     7861 ctgataccac catgctgcat agcgaagcga aacatccggt ttgcgcctac aaatggatga
     7921 actggtcatt aacgccaaaa gtgcagggcg atgtggcggc ctggtttggc tcgttaccgg
     7981 tagtgccgga agggtgtaaa gccagtccgt tattaggcga aaaagggtgt gaaaccaacg
     8041 gttttaacta tttcgacaaa atcgccttct ggaaaacgcc tatagcagaa gggggcaagt
     8101 ttgttcccta cagtcgctgg acgcaggatt acattgccat tatgggcggt cgctaacttc
     8161 gctggggtgc tttatgacgt acgcagtgga gtttgacaac gtctcgcggt tgtacggtga
     8221 cgtgcgcgca gtagatggcg tcagtattgc gataaaagat ggtgagttct tctctatgct
     8281 ggggccgtcc ggctccggca aaaccacctg cctgcgcctg attgctggct tcgaacagct
     8341 ttccggcggg gctatctcta tctttggtaa acccgccagc aatctgccac cgtgggagcg
     8401 ggacgtgaat actgtcttcc aggactacgc gctatttccg catatgtcga ttcttgacaa
     8461 tgtcgcctac gggctgatgg tcaaaggcgt gaataaaaag cagcggcacg caatggcgca
     8521 agaggcgctg gagaaagtgg cgctgggttt tgttcagcaa cgcaaaccgt cacaactttc
     8581 tggtggtcag cgccagcggg ttgctatcgc cagagcattg gtgaatgaac cgcgcgtatt
     8641 gctgttggat gaaccgctcg gcgcactgga tctcaaattg cgtgagcaga tgcagctgga
     8701 actgaaaaag ctgcaacagt ctctcggtat cacttttatc ttcgttaccc acgatcaggg
     8761 cgaagcgtta tcgatgtccg atcgtgtggc ggttttcaat aatggacgca ttgagcaggt
     8821 cgattccccg cgcgatctct atatgcgccc gcgcacgccg tttgttgccg gattcgtcgg
     8881 gacatccaac gtttttgatc cgctaatggc cgagaatctt tgcggcatga cgggaagctt
     8941 cgccctgcga ccggaacata tccgtctcaa tacccccggc gaaatgcagg ctaatggcac
     9001 gatccaggcg gtgcaatatc agggcgcagc gacccgtttt gaactgacac tgagcggcgg
     9061 tgaaaaactg ctggtgagtc aggccaatat gacaggcgaa gaactgcctg ccacgctcac
     9121 gcccggacaa caggtgatgg tttcctggtc gcgtgatgtg atggtgccgc tggttgagga
     9181 gaggtgaatg gcgatgaacg tattgcaatc accttcacgt ccaggtctgg gtaaggtgtc
     9241 cggtttcttc tggcggaatc cggggctggg gctgttttta ctgctgcttg gcccgctaat
     9301 gtggtttggc attgtctatt tcggctcgct gctgacactg ttatggcagg gcttttatac
     9361 ctttgacgat ttcaccatgt cggtaacgcc ggaactgacg ctggcgaaca tccgtgcgct
     9421 gtttaatccg gcaaattacg acatcattct ccgcacgctg actatggctg tggcagtcac
     9481 tatcgccagc gccattctgg cttttccaat ggcgtggtat atggcgcgct ataccagcgg
     9541 aaaaatgaaa gcgttttttt atatcgcggt aatgttgccg atgtgggcga gctacattgt
     9601 taaagcctat gcctggacgt tattgctggc aaaagatggc gtggctcagt ggtttttaca
     9661 acatctgggg ctggaaccac tgctgactgc gttccttaca ttacctgcgg tgggcggcaa
     9721 tacgctgtca acttccgggc tggggcgctt tctggtgttt ctctatatct ggctgccgtt
     9781 catgatcctg cccgtgcagg cggcgcttga gcgtttgccg ccgtcattgt tgcaggcgtc
     9841 agctgatctc ggcgcacgtc cacgacaaac ctttcgctat gtggtgctgc cgctggcgat
     9901 cccgggtatt gccgctggct ctatctttac cttctcactc acactgggtg attttatcgt
     9961 cccgcaactg gttggccctc caggatattt tatcggcaat atggtttatt cccagcaggg
    10021 ggcgattggc aatatgccga tggcggcggc attcaccctg gtgccgattg ttctcatcgc
    10081 actgtacctg gcgttcgtga aacgtctggg agcgttcgat gcactctgaa cgcgcaccgt
    10141 ttttcctcaa actggcggcc tggggcggcg tagttttcct acattttccc atcctgataa
    10201 tcgccgccta tgcgtttaac actgaagatg cggcgtttag ttttccaccg caaggcctga
    10261 cgctgcgctg gtttagcgtg gcagcacagc gtagtgatat tcttgatgcc gtgacactgt
    10321 cacttaaagt ggcggcgctg gcgacattaa tcgcgctggt gttagggacg cttgcagctg
    10381 ccgcgctgtg gcgacgagac tttttcggca aaaacgccat ttcgctgtta ctgctgctgc
    10441 ccattgcgct gccgggcatt gtcactggtc tggcgctatt aaccgctttt aaaaccatca
    10501 atctggagcc gggatttttc accatcgtgg tcggccatgc gactttttgc gtagtggtgg
    10561 tgtttaacaa tgtcatcgcc cgttttcgcc gcacctcgtg gagtctggtt gaggcgtcaa
    10621 tggatcttgg ggccaatggc tggcaaacct tccgctacgt agtgttgccg aatctcagtt
    10681 cggcgttact ggcaggagga atgctggcgt ttgccttgtc gttcgatgaa atcatcgtta
    10741 cgacctttac ggcaggtcat gaacgaacgt taccgttgtg gttgctcaat cagcttgggc
    10801 gaccgcgtga tgtaccggta actaacgtgg tggcactgct ggttatgttg gtaacaacct
    10861 tgccgatcct gggggcctgg tggctaaccc gcgaaggcga caatggtcaa taaccactga
    10921 tacaggaaaa tgctatgcaa cataagttac tgattaacgg agaactggtt agcggcgaag
    10981 gggaaaaaca gcctgtctat aatccggcaa cgggggacgt tttactggag attgccgagg
    11041 catccgcaga gcaggtcgat gctgctgtgc gcgcggcaga tgcagcattt gccgaatggg
    11101 ggcaaaccac gccgaaagtg cgtgcggaat gtctgctgaa actggctgat gttatcgaag
    11161 aaaatggtca ggtttttgcc gaactggagt cccgtaattg tggcaaaccg ctgcatagtg
    11221 cgttcaatga tgaaatcccg gcgattgtcg atgtttttcg ctttttcgcg ggtgcggcgc
    11281 gctgtctgaa tggtctggcg gcaggtgaat atcttgaagg tcatacttcg atgatccgtc
    11341 gcgatccgtt gggggtcgtg gcttctatcg caccgtggaa ttatccgctg atgatggcag
    11401 cgtggaaact ggctccggcg ctggcggcag ggaactgcgt agtgcttaaa ccatcagaaa
    11461 ttaccccgct gaccgcgttg aagttagcag agctggcgaa agatatcttc ccagcaggcg
    11521 tgattaatgt gctgtttggc agaggcaaaa cggtgggcga tccgctgacc ggtcatccca
    11581 aagtgcggat ggtgtcgctg acgggctcta tcgccaccgg cgagcacatc atcagccata
    11641 ccgcgccctc cattaagcgt actcatatgg aacttggtgg caaagcgcca gtgattgttt
    11701 ttgatgatgc ggatattgaa gcagtggtcg aaggtgtacg tacatttggc tattacaatg
    11761 ctggacagga ttgtactgcg gcttgtcgga tctacgcgca aaaaggcatt tacgatacgc
    11821 tggtggaaaa actgggtgct gcggtggcaa cgttaaaatc tggtgcgccg gatgacgagt
    11881 ctacggagct tggaccttta agctcgctgg cgcatctcga acgcgtcagc aaggcagtag
    11941 aagaggcgaa agcgacaggg cacatcaaag tgatcactgg cggtgaaaag cgcaagggta
    12001 atggctatta ctatgcgccg acgctgctgg ctggcgcatt acaggacgat gccatcgtgc
    12061 aaaaagaggt atttggtcca gtagtgagtg ttacgctctt cgacaacgaa gaacaggtgg
    12121 tgaactgggc gaatgacagc cagtacggac ttgcatcttc ggtatggacg aaagatgtgg
    12181 gcagggcgca tcgcgtcagt gcacggctgc aatatggttg tacctgggtc aatacccatt
    12241 tcatgctggt aagtgaaatg ccgcacggtg ggcagaaact ttctggttac ggcaaggata
    12301 tgtcacttta tgggctggag gattacaccg tcgtccgcca cgtcatggtt aaacattaag
    12361 gataatattg cagatcgtaa gagtatgaga tgatcttgcg tactgtcaga gcactgattt
    12421 ctctgacagt acgtgctgcg tgcaaaatca ctacatcaaa taaaccagcc aaatctcata
    12481 gagataagag taaacaaacc aacagcagca aagacattta tcagtactac agttctactg
    12541 gaaacattca tttttgccac cttttatcat tgcccctatg tgtatagtac gggttaagaa
    12601 aatccgcaat aatactcatt ctgaagatgg ataagggcaa gttgctgttt gatgtttttt
    12661 tctcaaaccg gttaaaaaac ttgatgtacg tcaaaaaatg acgcatattt gcgcgcgttt
    12721 tattcatctg gctggacgcc cgtacatgtc tctctatcaa cacatgcttg ttttttatgc
    12781 ggttatggca gcaatcgcat ttcttatcac ttggtttctt tctcacgata agaaacgcat
    12841 ccgtttctta agcgcctttc tggtgggggc aacatggccg atgagtttcc cggtggcgct
    12901 gttgttttca ctgttttaaa tcttactcac tggcttgacg gcaccacgca gacttatatc
    12961 atttggatga atcgataaat ttcacaagtg gctaaggaga aagtatgtcg catctggatg
    13021 aagtcatcgc gcgtgtggat gccgcgattg aagagagcgt cattgcccat atgaacgaat
    13081 tattaatcgc cctgagcgat gacgcggagt taagtcggga agatcgctac acccagcagc
    13141 agcgtttgcg caccgctatt gctcatcacg gtcgcaagca taaagaagat atggaagcgc
    13201 gccatgaaca gttaaccaaa ggcggcacca tcctgtgatt aaaatgagcg tctggctacc
    13261 aaccaggcgc caaccacaag taaaattgcg ccacaaattg ggccgaacaa cgcccgtagt
    13321 ggtacgccat gactgcggaa gatatccaga accagaccgc caatcagctg gctggcgacc
    13381 agtactgcaa tagttgtcgc cgctcccaca ttctgatatc cgctgatact ggcgaagaca
    13441 aaaaacgatc ccagcaagcc aggtataagt gtccaccagc gcacgctgga caccagttcg
    13501 ccaaaccccg ccataccttg tttaaaccat aaaatagaaa caaagagcac aatccccacc
    13561 agtgagttca gcaacatggc gatgagaatg gtagaggagg tctgggtgat gcgaaccatc
    13621 agcgtgttct ggactaccag accaattccg gcggcaatca aaaaggcaag ggtgagcgac
    13681 tgattcatcc aatcgcgtcc ggttcagtgc gttcgtcgag ttgcaactgc ataaatgtca
    13741 gatccagcca gcgcccaaat ttagtgccca cctgcggcat ttgtgcggtg acgacaaatc
    13801 ccagcgactg gtgcagatgc agcgaggcct gattttgcga ttcgatcccg gcgaccatga
    13861 catgcttccc gcaatcccgc gcttcatcaa tcaatcggct taacaattta cgccccagac
    13921 ctttgccctg atgatcggga tggacataaa ccgaatgttc cacggtatgg cgaaaaccat
    13981 cgaaactacg ccagtcgcca aatgaggcat atcccgtcac tacgccatct tcttcactta
    14041 ccagcaccgg ataacctgct atggtccgcg cttcaaacca ggcaatgcgg ttatctgcat
    14101 ccaccgtttg gtcattccag atagccgccg tatacaacac ggcgtggtta taaatttccg
    14161 caatggcagc acagtcggct ttgcgggcaa aacggatgga catgtctggc ctcaataaaa
    14221 taatgatgat gatgttatca atactatcga aaacagcccg aagcaacgga ttcctcgatg
    14281 aaattattgc gtgttgcaga ggggatgtga cggctgcaaa caaaaccaat cccgtgagcc
    14341 tgctactatt gatggattca tgccaaaagc acaggagagc attatggggc aacaaaagca
    14401 gcgtaatcga cgttgggttc tggcctcgcg tccgcatggc gcaccggttc cggagaattt
    14461 ccgtcttgaa gaagatgatg tcgccacacc gggtgaagga caggtgttac tgcgcacagt
    14521 ttatttgtcc ctggacccgt atatgcgtgg acgtatgagc gatgagccat cttattcacc
    14581 gccggttgat attggcggcg tgatggtcgg cggtacggtg agccgtgtcg tgaagtcgaa
    14641 tcatcctgat tatcagcctg gcgactgggt gctgggctac agtggatggc aggactatga
    14701 catatccagt ggtgatgatc tggtgaaact tggcgatcat ccgcaaaatc catcgtggtc
    14761 gctgggtgtg ctggggatgc caggctttac cgcttatatg gggctgctgg atatcggtca
    14821 gcctaaagag ggcgaaacgt tggtggtagc tgcggcgaca ggaccagtgg gggcgacggt
    14881 ggggcaaatc ggcaaactta aaggttgcag agtggtgggg gtagccggtg gcgcggaaaa
    14941 atgccgccat gctaccgagg tgttaggctt cgatgtttgc cttgatcacc acgcggacga
    15001 ttttgccgaa caactggcga aagcgtgccc aaaaggtatt gatatctatt atgaaaacgt
    15061 gggcggtaag gtatttgatg cagtgctacc attacttaat acatctgcgc gcattcccgt
    15121 ttgtggctta gtgagcagct ataacgctac agagctacca cccggtccgg atcgtttacc
    15181 cctgttgatg gcaacggtgc tgaaaaaacg tattcgcttg caagggttta ttatcgctca
    15241 ggattatggt caccgcatcc atgagtttca gaaggagatg gggcaatggg tgaaagagga
    15301 taaaatccac taccgcgaag aaattactga cggtttagag aatgcgccac agacgtttat
    15361 cggcctgctg aagggtaaaa acttcggcaa agtggtgatc cgcgtggcgg gtgatgatta
    15421 aagtttcaac ggcggcgtaa gccgccataa aaatccaggc aaaaagctta ttttcagctt
    15481 taattaacta accacatcat tgttctgctg ctaataacaa caaaggtgag tggtttatga
    15541 ttattcactt taatacacca ggtgaattcc ttctgccatg caggcagggt tggacagaaa
    15601 acacgcgaag gaattaaaat gcctggaacg gaaaaaatga aacatgtcag tttgactctg
    15661 caggttgaga acgacctgaa acatcagctt agtattggcg cactaaaacc tggcgcacgc
    15721 ctgattacta aaaatctggc ggagcaatta ggtatgagta ttacacctgt gcgtgaagca
    15781 ttattacgtc tggtttcggt gaatgcgctt tctgtcgcac cagcgcaagc atttacagtt
    15841 ccggaagtgg ggaaacgtca attagacgaa atcaatcgga tccgctacga actggaatta
    15901 atggcagttg ctctggctgt tgaaaacctc accccgcaag acctggcgga actccaggaa
    15961 ttgctcgaga aattacagca ggcgcaagaa aagggcgaca tggaacaaat cattaatgta
    16021 aacaggctat ttcgcttagc gatttatcat cgctcaaata tgcccatcct gtgtgagatg
    16081 attgagcaac tgtgggtcag gatgggacct ggtttacatt atctctatga agcgattaat
    16141 ccagcggaat tacgggagca tatagaaaac tatcatctgt tactcgctgc gttaaaagca
    16201 aaagacaaag agggatgcag acattgtctt gctgaaatta tgcaacaaaa tattgctatt
    16261 ttataccagc aatacaatcg ttaaagtaaa tgcccggcaa ataccgggca tcaaggtaat
    16321 tactcaaatc gccatgcgat attcacgccg acaccataat ttcgtccggg cgcaggttcg
    16381 taatatcgcc cgtttgactc attgacaatg acagaaccaa cgtattcttt atcgaataaa
    16441 ttatcgacac gaccaaataa atccacagtt aaattgtggt aattgtattt atacccggtg
    16501 aataagccga tgagagtata agacggcgcc ttggccgtat tttcatcatc tgccataata
    16561 tcgcccatat aacgcgcttc cgtgcctgca taccagccat cttccggtac ataacctatc
    16621 gacgcaaagc ccatattacg ggcgatccct ggcatccgat taccgttaca atcctgttca
    16681 ttgcaaacat tgctgcgata ggtcgcatca agccaggtcc aggacgcgtt tacgcgaaaa
    16741 tctcccgcga aacgttgatc ccatgccagt tcagcgcctt gacgacgggt ctttccagca
    16801 tttttgtaag tcgtacgccc accgctactg ctatcgacaa caatttcatc atcggtgtcg
    16861 gtctggaaca atgcgagact aagcagccca tcaccaatac gcgttttact gccgatctca
    16921 attgtatcgt tggtggatgg ttttaaacca aagttcatac cgctttgccc atcagcgcga
    16981 taagacagct cattaatcgt cggcgtttca aaaccacgcc cggctgccag atagatattc
    17041 caggcatcgg tcattgcata ttttaacgat ccggctggta gccatttgtg ataactggca
    17101 tcaccgctgt catcgccgtt acccggagta acgtaatggt cgttggaatc aaaccacacg
    17161 gagctgtagc gcacgccagc atccagcgac agtttttcgc tcagctgcca ctgcgtctgt
    17221 aaatagggat cgatgttcca catcagattg cgttcgtcgc gacgcaactc acctttttgc
    17281 ccgtactccg gcatgccgct attcaggcgg aagttattgt agcccttgcg gttttcactc
    17341 atgttttcgt agttcaggcc ggtagtgaac gtgaccggaa cgcccagttc accacggtgt
    17401 gtccagcggc tgtctattcc ctggtaatgg cgttgcaggg taatcacgcc gcccgcatgt
    17461 gacgggttaa gttgtggcgc catgggtatt gactggtact gggtcgtttc tcgctctccg
    17521 gcatacatca tcacactcat atcatcccgc gaactcaggc tacgttcata gcgcaaccca
    17581 gcctgagttt gcttgatggt ttttcgcgtg tcgtactgtt ctgcacgagg cgcttgttgt
    17641 ggattcgcct tccattctgc tttggttagc ccacccgggt catctgcttt gatatccaca
    17701 ctattgaaaa tcaggcttaa tttgctggct tcatcaatgc gtacgcccag tttggcattg
    17761 gctaaatttt tctgtgcgcc actatggtca cgatagccgt gggtcgtaaa acgcgtggtt
    17821 gagacggtgt aatcaacatc gccgggctgt gtgccgtctc ccgttgcgcc cgttgctttc
    17881 agcccatagc gccagctgcc aaaactgccg tagtaactac tggcttcaat ggttggtggc
    17941 tgttgtccgg tctgggtggt gacattcatt acaccgccag acgcgttgcc atacagggca
    18001 gagaaggggc cacgcagcac ttccacattt tgcacactgc ttaaatcgat gttggatgtt
    18061 tgcccttgcc cgtcgggcat ggtggcggga ataccgtcca catacaggcg aataccgcga
    18121 attccgtaag tggaacggga gccaaatccg cgaatcgaca gctgtaaatc ttgcgcatag
    18181 ttctgccggt tttgtacctg caaaccaggc acgccggtca gtgattcgga caagttaatg
    18241 cgcggtgttg ccaggcgcat ctcctcgcca tccaccacgc ttactgctgc aggggtatcc
    18301 agttctgaaa ccacctgcgg cgcggcactg acaatcatgg tctgttcatc agcggcaaaa
    18361 acaacggggg aaaggacaag cagggcgggc aaaacggtct gtcggacgga aaaaatcttc
    18421 atgaaaaaag ccaggttaag aatgggaaaa cgccgtcatg gtaatgaaat tgtaaattta
    18481 tggaaaatga aacggcacaa tacgttaagt aattgagaaa attgtagtcg taacggcaag
    18541 aaatgctcca catttgagaa aataatgatt accattccca tttataacaa gagcgtaacg
    18601 atgattacgc ttagcgaagc attgtgaagc agcaaaaata tcggttcatc aaagggagtc
    18661 gtcatgcatt tacgtcatct gttttcatcg cgcctgcgtg gttcattact gttaggttca
    18721 ttgcttgttg cttcatcatt cagtacgcag gccgcagaag aaatgctgcg taaagcggta
    18781 ggtaaaggtg cctacgaaat ggcttatagc cagcaagaaa acgcgctgtg gctcgccact
    18841 tcgcaaagcc gcaaactgga taaaggtggc gtggtttatc gtcttgatcc ggtcactctg
    18901 gaagtgacgc aggcgatcca taacgatctc aagccgtttg gtgccaccat caataacacg
    18961 actcagacgt tgtggtttgg taacaccgta aacagcgcgg tcacggcgat agatgccaaa
    19021 acgggcgaag tgaaaggccg tctggtgctg gatgatcgta agcgcacgga agaggtgcgc
    19081 ccgctgcaac cgcgtgagct ggtagctgat gatgccacga acaccgttta catcagtggt
    19141 attggtaaag agagcgtgat ttgggtcgtt gatggcgaga atatcaaact gaaaaccgcc
    19201 atccagaaca ccggtaaaat gagtaccggt ctggcgctgg atagcaaagg caaacgtctt
    19261 tacaccacta acgctgacgg cgaattgatt accatcgaca ccgccgacaa taaaatcctc
    19321 agccgtaaaa agctgctgga tgacggcaaa gagcacttct ttatcaacat tagccttgat
    19381 accgccaggc agcgtgcatt tatcaccgat tctaaagccg cagaagtgtt agtggtcgat
    19441 acccgtaatg gcaatattct ggcgaaggtt gcggcaccgg aatcactggc tgtgctgttt
    19501 aacccagcgc gtaatgaagc ctacgtaacg catcgtcagg caggtaaagt cagtgtgatt
    19561 gacgcgaaaa gctataaagt ggtgaaaacg ttcgatacgc cgactcatcc gaacagcctg
    19621 gcgctgtctg ccgatggcaa aacgctgtat gtcagtgtga aacaaaaatc cactaaacag
    19681 caggaagcta cccagccaga cgatgtgatt cgtattgcgc tgtaatagat atggcgaggg
    19741 tgaaaacgga gagacttgcc tgacttttat cacgccgcat gcgacacctt cgtcagtaat
    19801 ctaaatttgc ccggcacgta gccgggcaat tttgcaatta gctcgccgtt tcaggcttaa
    19861 acacaatttc ctgtttttct tcatcttcct cgacgactgg cgcagtgctg tgaatttcag
    19921 caacgcgttt gcgcacacca aaccagccta taaccagcag aataccgata atcggcagcg
    19981 ccgcgatagt ataagtcccg ttcgggtaat cgaacgccat cagcacaagg acactcagta
    20041 aaaacagtaa tgtcagccag gaagtgaagg gcgcgccagg cagtttaaaa ctgacatccg
    20101 ctgctttgcc tgctttaatc gctttacgca ggcgcatctg gcacacgatg ataaacgccc
    20161 atgaagcgat gattcccagc gacgcgaagt tcaacacaat ctcaaatacg cgcgacggca
    20221 ccagatagtt gaggaatacg ccgacgacat acacaaccag tgtcgccaga atcccggcat
    20281 acggcacatg ctgacgactc attttcgcca taaaactcgg tgcggaaccg cccatcgcca
    20341 ttgagcgcag aatacgtccg gtgcagtaca gacctgaatt caggctggag agggcagcgg
    20401 tcagcaccac aatgttcata atgctgccga tatatggcac acccagttta gagaaaaacg
    20461 tcacgaacgg actttgcccc gcctgatacg cgctccacgg caataacata accagcaaca
    20521 ccacggagcc gacgtaaaac aggccaatac gccaaatcac actgttaatg gctttaggca
    20581 ccatggtctg cggatcttta cattctccgg cagctgtacc caccatttca atggaggcaa
    20641 aagcaaacac tacgccctga atcaacacca gcgcaggcag caaaccgtgg gggaagaagc
    20701 cgccattatc ggtaattaaa tgaaagccag tggtgttgcc atccagcggc tgaccactac
    20761 cgaggtacac tgtacccacg accaaaaagg tcacaatggc gagcacttta ataagcgcaa
    20821 accagaactc catctccgca aaccatttca caccgatcat attcatggtg ccaacgatgg
    20881 taagtgcagc gagcgcaaag acccactgcg gcacgccgcc aaacgcaccc cagtaatgca
    20941 tatacagagc gacggcggta atatcaacaa tccccgtcat cgcccagttg atgaagtaca
    21001 tccagccagc aacataagcg gctttctcac ccaaaaactc acgggcataa gagacaaaac
    21061 tgccactgga agggcggtgt agcaccagct cacccaatgc acgcagaata aaaaacgaaa
    21121 acaagccaca aattaaataa accagtgcca gtgctggacc cgccatttgc agtcgggctc
    21181 ctgcacctaa aaacaagccg gtgccaatcg cgccgccaat ggcgatcatc tgtacctggc
    21241 gattgcccat cgctttgtga tacccctctt cgtgggcatt aagccagcgg cgtttcgcgg
    21301 cgtgttgatc tgaagtgtcg gtgtcgtgtt tactcattgc tctccctgat tgctttaatg
    21361 aaaaagtcat ataagttgcc atgaacaatg tttattcatc ctggcgatga tagttatcac
    21421 cattattctt tatgggtata aaactatcgg cgcagcatcc tacccgttat cgataaacga
    21481 tgcaaaacat ccccttacaa tcctgaaggg gattaataca actgacgaaa aaatgacaaa
    21541 tccttttgct ggttaacctg tgtactgtcc tacacttaat ctttaaaaga ttgtgagggg
    21601 catatgatta aggtgtatgg cgtacccgga tggggctcta caatcagtga gctgatgcta
    21661 acactggctg atattcctta tcagttcgtt gatgtcagtg gttttgacca cgaggggact
    21721 tcacgcgagt tattgaaaac cctgaacccg ttgtgtcaga ttccgacact ggcgctggaa
    21781 aatgacgaga ttatgacgga gacagcggcg attgcattga tggttctcga tcgtcgcccg
    21841 gaccttgcgc caccggttgg gcgcgccgaa cgtcagcagt ttcaacggct attggtctgg
    21901 ctggttgcta atgtctatcc aacattcact ttcgccgatt accctgagcg ttgggctcct
    21961 gacgcacccg aacagttaaa gaagaatgtt attgaatatc gtaaatcgct ttatatctgg
    22021 ctgaattcac aattgaccgc tgaaccttat gcgtttggtg agcaactaac gctggtggac
    22081 tgttatcttt gcactatgcg cacatggggg cctgggcatg aatggtttca ggataatgcc
    22141 acgaatatca gtgcgattgc tgatgctgta tgtcagttac cgaaattaca agaagtatta
    22201 aaaaggaatg tcattattta acaataatgc cggaggttaa tgttaatgaa tcactggcgt
    22261 agttaactgg atat
//
LOCUS       DAZFFG010000080        22240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFFG010000080 DAZFFG010000000
VERSION     DAZFFG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.080"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            243..578
                     /locus_tag="ACHJ7J_003324"
     CDS             243..578
                     /locus_tag="ACHJ7J_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO5001515.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            1136..1579
                     /gene="yjdN"
                     /locus_tag="ACHJ7J_003325"
     CDS             1136..1579
                     /gene="yjdN"
                     /locus_tag="ACHJ7J_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO5001516.1"
                     /translation="MPLSPYISFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1712..2500
                     /gene="phnC"
                     /locus_tag="ACHJ7J_003326"
     CDS             1712..2500
                     /gene="phnC"
                     /locus_tag="ACHJ7J_003326"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO5001517.1"
                     /translation="MQTIIRVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLIVGDKKPECRVELLGRTVQQNGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNDRFDHLYRSINRVEKNAKAA"
     gene            2525..3541
                     /gene="phnD"
                     /locus_tag="ACHJ7J_003327"
     CDS             2525..3541
                     /gene="phnD"
                     /locus_tag="ACHJ7J_003327"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5001518.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLARRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3647..4426
                     /gene="phnE"
                     /locus_tag="ACHJ7J_003328"
     CDS             3647..4426
                     /gene="phnE"
                     /locus_tag="ACHJ7J_003328"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO5001519.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVILSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCAMMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4447..5172
                     /gene="phnF"
                     /locus_tag="ACHJ7J_003329"
     CDS             4447..5172
                     /gene="phnF"
                     /locus_tag="ACHJ7J_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO5001520.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            5173..5625
                     /gene="phnG"
                     /locus_tag="ACHJ7J_003330"
     CDS             5173..5625
                     /gene="phnG"
                     /locus_tag="ACHJ7J_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO5001521.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLADGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5622..6206
                     /gene="phnH"
                     /locus_tag="ACHJ7J_003331"
     CDS             5622..6206
                     /gene="phnH"
                     /locus_tag="ACHJ7J_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO5001522.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVADEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            6206..7270
                     /gene="phnI"
                     /locus_tag="ACHJ7J_003332"
     CDS             6206..7270
                     /gene="phnI"
                     /locus_tag="ACHJ7J_003332"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO5001523.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALACSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            7263..8108
                     /gene="phnJ"
                     /locus_tag="ACHJ7J_003333"
     CDS             7263..8108
                     /gene="phnJ"
                     /locus_tag="ACHJ7J_003333"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO5001524.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            8105..8863
                     /gene="phnK"
                     /locus_tag="ACHJ7J_003334"
     CDS             8105..8863
                     /gene="phnK"
                     /locus_tag="ACHJ7J_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO5001525.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(8818..9093)
                     /locus_tag="ACHJ7J_003335"
     CDS             complement(8818..9093)
                     /locus_tag="ACHJ7J_003335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001526.1"
                     /translation="MEHHAFARVDGERGAVEDGQAHAVLLVQDEGFTDVLYVNHFSPR
                     GSGVGCDADASYPTYGERICRPDKAFTPHPAPAQFCKTDDTSSCVYG"
     gene            8974..9654
                     /gene="phnL"
                     /locus_tag="ACHJ7J_003336"
     CDS             8974..9654
                     /gene="phnL"
                     /locus_tag="ACHJ7J_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO5001527.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVNLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9651..10787
                     /gene="phnM"
                     /locus_tag="ACHJ7J_003337"
     CDS             9651..10787
                     /gene="phnM"
                     /locus_tag="ACHJ7J_003337"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO5001528.1"
                     /translation="MIINNVKLVLENEVVSGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10787..11344
                     /gene="phnN"
                     /locus_tag="ACHJ7J_003338"
     CDS             10787..11344
                     /gene="phnN"
                     /locus_tag="ACHJ7J_003338"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO5001529.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYASALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11331..11765
                     /gene="phnO"
                     /locus_tag="ACHJ7J_003339"
     CDS             11331..11765
                     /gene="phnO"
                     /locus_tag="ACHJ7J_003339"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO5001530.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNIGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            11767..12525
                     /gene="phnP"
                     /locus_tag="ACHJ7J_003340"
     CDS             11767..12525
                     /gene="phnP"
                     /locus_tag="ACHJ7J_003340"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO5001531.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAVTLIDAGLHDLADRWLPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12621..12950)
                     /gene="yjdP"
                     /locus_tag="ACHJ7J_003341"
     CDS             complement(12621..12950)
                     /gene="yjdP"
                     /locus_tag="ACHJ7J_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO5001532.1"
                     /translation="MKRHSTLFFFTLLTLTTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(13019..15280)
                     /locus_tag="ACHJ7J_003342"
     CDS             complement(13019..15280)
                     /locus_tag="ACHJ7J_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5001533.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNRELRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     AQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAYGEADVT
                     FPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQALGQEN"
     gene            15394..16914
                     /locus_tag="ACHJ7J_003343"
     CDS             15394..16914
                     /locus_tag="ACHJ7J_003343"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5001534.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16911..17885
                     /locus_tag="ACHJ7J_003344"
     CDS             16911..17885
                     /locus_tag="ACHJ7J_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5001535.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            17904..18845
                     /locus_tag="ACHJ7J_003345"
     CDS             17904..18845
                     /locus_tag="ACHJ7J_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5001536.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18900..19583
                     /locus_tag="ACHJ7J_003346"
     CDS             18900..19583
                     /locus_tag="ACHJ7J_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO5001537.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGTQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19598..20458
                     /locus_tag="ACHJ7J_003347"
     CDS             19598..20458
                     /locus_tag="ACHJ7J_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFO5001538.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHAIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20455..21456
                     /locus_tag="ACHJ7J_003348"
     CDS             20455..21456
                     /locus_tag="ACHJ7J_003348"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5001539.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21453..22160)
                     /locus_tag="ACHJ7J_003349"
     CDS             complement(21453..22160)
                     /locus_tag="ACHJ7J_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5001540.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALVVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
BASE COUNT         4830 a         6582 c         6313 g         4515 t
ORIGIN      
        1 agaaagataa aaatatcacg tgaattaaaa aaagaacaag tagagcatta accttatctt
       61 aaataataaa tagaggcaaa aagattattt tctttttgcg ttttctttca aatgaaaacg
      121 atcgtcgtct aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg
      181 cctttttcgg caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc
      241 acatgtcatt accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca
      301 tgtacatctg cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg
      361 acgagctgat cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca
      421 tcattaaaga tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga
      481 aaaatatccg cctggttgaa ggcgatcata acatcgattg caaaatcgac ggttttggtc
      541 cgatgaaact gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg
      601 gataaggcgt ttacgccgca tccggcaacg gtgccagatg cctgatgcga cgctcgcgcg
      661 tcttatcagg cctacaaatt cccgcaccct ccgtaggccg gataaggcgt ttacgccgca
      721 tccggcaacg gtgccgactg cctgatgcga cgctcgcgcg tcttatcagg cctacaaatt
      781 cccgcaccct ccgtaggccg gataaggcgt ttacgccgca tccggcaacg gtgccagatg
      841 cctgatgcga cgcttgcgcg tcttatcagg cctacaaatt cccgcaccct ccgtaggccg
      901 gataaggcgt ttacgccgca tccggcaacg gtgccgactg cctgatgcga cgcttgcgcg
      961 tcttatcagg cctacaaatt cccgcaccct ccgtaggccg gataaggcgt ttacgccgca
     1021 tccggcaaca gtgccgactg cctgatgcga cgctcgcgcg tcttatcagg ccgcctctca
     1081 tctgtataaa tttcgaacta cacttaactg gcttctcttc actgaggtca ccatcatgcc
     1141 gttaagtccc tacatctctt ttgccggtaa ctgttccgac gcgattgcct attatcaacg
     1201 cacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccaa aatcagcgca
     1261 ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca tcgctcatgc
     1321 caacgtgcgc attgccggaa gcgacatcat gatgagcgat gccatgccgt caggaaaagc
     1381 cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag gaaaacgctg
     1441 gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa ctttctgggc
     1501 gcatggcttc ggcaaagtca ccgataaatt tggcgtaccg tggatgatta atgtcgtcaa
     1561 acaacaacca acgcaataat ccgccgggag gcccgccctc ccgcactgtc atcgaattcc
     1621 cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat aatccgccac
     1681 gatggagcca cttttttagg gaggctccag catgcaaacg attatccgcg tcgaacaact
     1741 gagcaaaacc ttcaatcagc atcaggcgct gcacgcggtt gatctgaata tccatcatgg
     1801 tgaaatggtg gctctgcttg ggccgtccgg ttccggcaaa tccacccttt tacgtcactt
     1861 aagcggcctg attgtcggcg ataaaaagcc agaatgccgc gtcgaactgc tgggccgcac
     1921 cgtccagcaa aatggccgcc tggcccgcga tatccgcaaa agccgcgcca acaccggcta
     1981 catcttccaa caattcaacc tggtgaatcg cctgagcgta ctggagaacg tgctgattgg
     2041 cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccc gcgagcagaa
     2101 acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc atcagcgcgt
     2161 ttccaccctc tccggcggcc agcagcagcg tgtagcgatt gcccgcgcgc tgatgcagca
     2221 ggcgaaggtg attctcgccg acgaaccgat cgcctcgctg gacccggaat cggcgcgcat
     2281 cgtgatggac accctgcgcg acatcaacca gaacgacggc atcaccgtgg tcgtcacgct
     2341 gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc gtcaggggca
     2401 cgtcttctac gacggcagca gccaattgtt tgataacgat cgttttgacc atctctaccg
     2461 cagcattaac cgcgtcgaaa agaacgcgaa agctgcctga catccctatc attgaggaaa
     2521 acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca gcctcagcac
     2581 cctgttaacc ccggcgcatg ctgaagagca ggaaaaggcg ctgaatttcg gcattatttc
     2641 aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg atatggagaa
     2701 gaagctgggc gtgaaggtca acgccttctt tgccccggac tatgcgggca ttatccaggg
     2761 aatgcgtttc aacaaagtgg atatcgcctg gtacggcaac ctgtcggcga tggaagcggt
     2821 ggatcgcgcc aacggtcagg tcttcgccca gacggtcgcg gcggatggat cgccgggtta
     2881 ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgaacg atctgctggc
     2941 caggcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg gcttcctcgt
     3001 ccccggctac tacgtcttcg ccaaaaacaa tatctccgcc agcgacttca agcgcaccgt
     3061 caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaggtgg atgttgccac
     3121 taacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagt tgaaagaact
     3181 gaaagtgatc tggaagtcgc cgctgatccc aggcgatccg atcgtctggc gtaaaaacct
     3241 ttccgaaacc accaaagaca agatctacga cttctttatg aactacggca agacgccgga
     3301 agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcctcca gcgacctgca
     3361 actggtgccg attcgccagc tcgcgctgtt taaagagatg cagggcgtga aaagcaataa
     3421 aggactgaat gagcaggaca agctggcaaa aaccaccgag attcaggcgc agctggatga
     3481 cctggaccgc ctgaacaacg cgctaagcgc gatgagttcg gtgagtaaag cggtgcagta
     3541 aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgcagg cgttgatgcc
     3601 ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc aaaccatcac
     3661 catcgcccca cccaagcgca gctggttctc gcttctgagc tgggccgttg tactcgccgt
     3721 gttggtcgtc tcgtggcagg gcgcggaaat ggccccgctc acgctgatca aagacggcgg
     3781 caacatggcg accttcgccg ccgacttctt cccgcccgat ttcagccagt ggcaggatta
     3841 cctcaccgaa atgctcgtca cgctgcaaat cgccgtctgg ggcaccgcgc tggcggtgat
     3901 tctctccatc cccttcggcc tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca
     3961 gcccgttcgc cgcctgatgg acgcctgccg cgccattaac gaaatggtct tcgccatgct
     4021 gttcgtggtc gccgtcggcc tcggcccgtt cgctggcgtg ctggcgctgt tcattcacac
     4081 caccggcgtg ctctccaaac tgctttccga agcggtggaa gcgattgaac ccggcccggt
     4141 ggagggcatt cgcgccaccg gtgccaacaa gctcgaagag atcctctacg gcgtgctgcc
     4201 gcaggtgatg ccgctgctga tctcctactc cctctatcgc ttcgaatcca acgtccgctc
     4261 ggcgaccgtc gtcggcatgg tcggcgcggg cgggatcggc gtcacgctgt gggaagcgat
     4321 tcgcggtttc cagttccagc aaacctgcgc catgatggtg ctgattatcg tcacggtcag
     4381 cctgctggat ttcctctctc aacggttgcg taagcacttt atctgattag cgaggcattg
     4441 atagctatgc acttgtctac acatccgacc agctacccaa cacgctatca ggagatagcc
     4501 gcaaaacttg agcaggagct tcgtcaacac taccgctgcg gcgactatct tcccgccgag
     4561 cagcagctgg cggcgcgctt tgaggtgaac cgccacactc tgcgccgcgc catcgaccaa
     4621 ctggtggaaa agggctgggt acagcgccgt cagggcgtcg gcgtactggt gctgatgcgc
     4681 ccgttcgatt acccactcaa cgcccaggcg cgttttagcc agaacctgct ggatcagggc
     4741 agtcatccca ccagcgaaaa actgctgtct gtgctgcgcc cggcctccgg tcacgtcgct
     4801 gacgcgctgg ggataaccga gggggagaac gtcatccacc tgcgcaccct gcgccgggtc
     4861 aacggcgtcg cgctctgttt aatcgaccac tacttcgcgg acctcaccct ctggccgacg
     4921 ctgcaacgct tcgacagcgg ctcgctgcac gattttctgc gcgagcaaac gggaattgcg
     4981 ctgcgccgca gccagacgcg gatcagcgcc cgccgcgccc aggccaaaga gtgtcagcgc
     5041 cttgaaatcc cgaatatgtc gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt
     5101 gaaagcagcc cggcggagta ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact
     5161 atggagcact gaatgcacgc agataccgcg acccgccagc actggatgtc cgtactggcg
     5221 cacagccaac cggctgagct ggcagcccgc ctgaacgcgc tgaacatcac cgccgactat
     5281 gaagtgatcc gcgccgccga aaccggcctg gtgcagattc aggcgcggat gggcggcacc
     5341 ggcgaacgct tttttgccgg cgacgccacg ctcacccgcg ccgccgtgcg cctggctgac
     5401 ggcacgctcg gctacagctg ggtgctgggg cgcgacaaac agcacgccga acgctgcgcg
     5461 ctgattgacg cgctgatgca gcaaacccgc cactttcaaa acttatcaga aacccttatt
     5521 gccccgctgg acgctgaccg tatggcacgc attgccgcac gccaggccga agtgaacgcc
     5581 agccgggtcg acttctttac gatggttcgc ggagacaacg catgaccctg gaaaccgctt
     5641 ttatgcttcc cgtgcaggat gcccagcaca gttttcgtcg cctgttaaag gccatgagcg
     5701 agccgggcgt gattgtcgcc ctgcatcagc tcaaacgcgg ctggcagccg ctgaatatcg
     5761 ccaccaccag cgtgctgctg acgctggccg ataacgacac gccggtgtgg cttgccgcgc
     5821 cattgagcaa cgatatcgtc agccagagcc tgcgttttca taccaacgcg ccgctggtca
     5881 gccagccgga acaggcgacc ttcgcggtag cggatgaggc gatttccagc gaacagctca
     5941 acgccctttc caccggcacc gccgttgcgc cggaagcggg cgcgacgctg attttacaag
     6001 tcgccagcct gagcggcggg cgcatgctgc gcctcaccgg tgcgggcatt gccgaagaac
     6061 gaatgatcgc cccgcagctg ccggagtgca ttctgcacga actcaccgag cgcccgcacc
     6121 cgttcccgct cggcatcgac ctgatcctga cctgcggcga gcgcctgctg gctgttccgc
     6181 gaaccactca tgtggaggtg tgctgatgta cgttgccgtg aaagggggcg agaaggcgat
     6241 cgacgccgcc cacgccctgc aagagagccg acgccggggc gataccgatt tgcctgaact
     6301 gagcgtcgcc cagattgaac agcagcttaa tcttgcggtg gatcgcgtga tgaccgaagg
     6361 cggcattgcc gaccgcgaac tggcggcgct ggcgctgaaa caggccagcg gcgataacgt
     6421 cgaagcaatt ttcctgctgc gcgcctaccg caccacgctg gcgaaactcg cggtaagcga
     6481 gccgctcgac accacggaga tgcgtctcga acggcgtatt tcagcggttt ataaagatat
     6541 tcccggcggc cagttgcttg gcccaaccta cgactacacc catcgcctgc tcgatttcac
     6601 cctgctggca aacggcgaag cgccgacgct gaccaccgcc gacagcgaac aacagccgtc
     6661 gccgcacgtt ttcagcctgc tggcgcgtca ggggctggcg aagtttgaag aggatagcgg
     6721 cgcacagccg gatgacatca cccgcacgcc gccggtttac ccctgctcac gttcttcccg
     6781 tttgcagcag ttgatgcgcg gcgacgaagg ctatctgctg gcgctggcct gctccaccca
     6841 acgcggctac gggcgcaacc acccgttcgc aggcgagatc cgcagcggct atatcgacgt
     6901 atcgattgtg ccagaagagc tgggatttgc ggtaaacgtc ggcgaactgc tgatgaccga
     6961 gtgtgaaatg gtcaacggtt ttatcgaccc gccggatgag ccgccgcact tcacgcgcgg
     7021 ctacgggctg gtattcggca tgagcgagcg caaagcgatg gcgatggcgc tggttgaccg
     7081 cgctctgcaa gccccggagt acggcgagca cgcgacaggc ccggcgcagg atgaagagtt
     7141 cgtgctggcg catgccgaca acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc
     7201 gcactacgtc gatttccagg ccgaactgga gctactcaaa cgtctgcaac aggagcagaa
     7261 ccatggctaa tctgagcggc tacaactttg cctacctcga cgagcagacc aaacgcatga
     7321 tccgccgcgc catcttaaaa gcggtggcga tccccggtta tcaggtacct tttggcgggc
     7381 gcgagatgcc aatgccgtac ggctggggaa ccggtggtat ccagctcacc gccagcgtga
     7441 ttggcgaaag cgacgtgttg aaggtgattg accagggcgc ggatgacacc accaacgccg
     7501 tatcgattcg caacttcttc aagcgcgtga ccggggtaaa caccactgaa cgtacggacg
     7561 atgccacggt catccagaca cgtcaccgca tccccgaaac gccgctgacc gaagatcaaa
     7621 tcattatttt ccaggtgccg atcccggaac cgctgcgctt tatcgagccg cgcgaaacgg
     7681 aaacccgcac catgcacgcg ctggaagagt acggcgtgat gcaggtgaag ctgtatgaag
     7741 atatcgcccg cttcggtcat atcgccacca cctacgccta tccggtgaag gtgaacgggc
     7801 gctacgtaat ggacccgtcg ccgatcccga aattcgataa cccgaaaatg gacatgatgc
     7861 ccgccctgca gctctttggt gcggggcgtg agaagcgcat ctatgcggtg ccgccgttta
     7921 cccgcgtgga aagcctcgat ttcgacgatc acccgttcac cgtccagcag tgggatgaac
     7981 catgcgccat ctgcggatcg acccacagct atcttgatga agtggtgctg gatgacgccg
     8041 gaaaccgcat gtttgtctgc tccgataccg attattgccg ccaacagagc gaggcgaaaa
     8101 gccaatgaat caaccgttac tttcggtcaa taacctgacc cacctttacg cgccgggcaa
     8161 aggctttagc gatgtctctt ttgatttatg gccgggagaa gtgctgggca tcgtcgggga
     8221 gtccggctcc gggaagacca cgctgctgaa atcaatctcc gcgcgcctga cgccgcaaca
     8281 gggggagatt cactatgaga accgttcgct gtacgcaatg agcgaagccg accgccgtcg
     8341 cctgctgcgc accgaatggg gcgtggtgca tcagcatccc ctcgacggcc tgcgccgcca
     8401 ggtctcggca ggcggcaata tcggcgagcg gctgatggcg accggggcgc gtcattacgg
     8461 cgacattcgc gccaccgcgc agaagtggct ggaagaggtg gaaattcccg ccaaccggat
     8521 cgacgacctg ccgaccacct tttccggcgg catgcagcag cgtttgcaga ttgcccgcaa
     8581 tctggtgacg catccgaagc tggtgtttat ggacgagcca accggcgggc tggatgtgtc
     8641 ggtgcaggcc cgcctgctcg atctgctgcg cggcctggtg gtggagctga acctcgcggt
     8701 agtgattgtc acccacgatt taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat
     8761 gaagcagggg caagtggtgg agagtgggtt aaccgaccgc gtgctcgacg acccgcatca
     8821 tccgtataca cagctgctgg tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg
     8881 cgtaaacgcc ttatccggcc tacaaatgcg ctccccgtag gtcggataag acgcatcagc
     8941 gtcgcatccg acacccgaac cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa
     9001 accttcatcc tgcaccaaca aaacggcgtg cgcctgcccg tcctcaaccg cgcctcgctc
     9061 accgtcaacg cgggcgaatg cgtggtgctc catggccact ccggcagcgg caaatcaacc
     9121 ctgctgcgct cgctgtacgc caactatctg cccgacgaag ggcaaatcca gatcaaacac
     9181 ggtgacgaat gggtaaacct cgtcaccgcc ccagcgcgca aagtggtgga aatccgcaaa
     9241 accaccgttg gctgggtgag ccagtttctg cgcgtcatcc cgcgcatttc ggcactggaa
     9301 gtggtgatgc aaccgctgct cgataccggc gtaccgcgtg aagcctgcgc cgctaaagcc
     9361 gcgcgtctgc tcacccgcct gaacgtgccg gaacggctgt ggcatctggc gccatcgacc
     9421 ttctccggcg gcgaacagca gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc
     9481 attctgctgc tcgacgaacc taccgcctcg ctggacgcca aaaacagcgc cgcggtggtg
     9541 gaactgatcc gcgaagccaa agcccgcggc gcagccattg tcggcatctt ccatgacgaa
     9601 gccgtacgta atgacgttgc cgaccgcctg cacccaatgg gagcctcttc atgattatca
     9661 ataacgtcaa attggtgctg gaaaacgagg tggtaagcgg ttcgctggag gtgcaggacg
     9721 gcgaaatccg cgcctttgcc gaaagccaga gccgcctgcc ggaggcgatg gacggcgaag
     9781 gcggctggct gctgccgggg ctgattgagc tgcataccga taatctggat aaattcttca
     9841 ccccgcgccc gaaagttgac tggcccgccc actcggcaat gagcagccac gacgcgctga
     9901 tggtggcgag cggcatcacc accgtactgg atgccgtggc aattggcgac gtgcgcgacg
     9961 gcggcgatcg gctggagaat ctggagaaga tgatcaacgc catcgaagag acgcagaaac
    10021 gcggcgtcaa ccgcgccgag caccgcctgc acctgcgctg cgagctgccg catcacacca
    10081 cgctgccgct gtttgaaaaa ctggtgcagc gcgagccggt gacgctggta tcgctgatgg
    10141 accactcgcc gggccagcgc cagttcgcca accgcgagaa gtatcgcgag tattatcagg
    10201 gtaaatactc gctcactgat gcgcagatgc agcagtatga agaggaacag ctggcgcttg
    10261 ctgcacgctg gtcgcagcca aatcgcgaat ccatcgccgc aatgtgccgc gcgcgacaca
    10321 ttgcgctcgc cagccacgat gacgccacgc acgcccacgt tgccgaatcc catcagcttg
    10381 gcagcgtgat cgccgaattt cccaccacgt tcgaagcggc agaagcctcg cgcaagcatg
    10441 gtatgaacgt gctgatgggc gcgccgaata tcgtacgtgg cggttcgcac tccggcaacg
    10501 tggcggccag tgaactggcg cagcttggcc tgctggatat cctctcttcc gactactacc
    10561 ccgccagcct gctggatgcg gcgttccgcg tggccgatga ccagagcaac cgctttacgc
    10621 tgccgcaggc ggtgaagctg gtgaccaaaa atccggccca ggcgctcaat ctccaggatc
    10681 gcggggtgat tggcgagggc aaacgcgccg acctggtgct ggcgcatcgc aagggcaacc
    10741 acattcatat cgaccacgtc tggcgtcagg gtaaaagggt gttctgatga cgggaaaact
    10801 gatttggtta atggggccgt ccggctccgg gaaagatagc ctgctggcgg aactccgcct
    10861 gcgggaacaa actcagttac tggtggcgca tcgctacatc acgcgcgatg ccagcgctgg
    10921 aagcgaaaac catatcgccc tgagtgagca ggagtttttt acccgcgcgg ggcaaaatct
    10981 gctggcctta agctggcacg ccaacggtct gtattatggc gtcggcgtcg agatcgacct
    11041 ctggctgcac gccggattcg acgtgctggt caacggctca cgcgcccatt tgccgcaggc
    11101 gcgggcacgc tatgcatcgg cgctgctgcc catctgctta caggtgtcgc cagagatcct
    11161 gcgccagcgc ctggaaaacc gtggtcgtga aaacgccagt gaaattaacg cccggctggc
    11221 gcgcgccgcc cgctatactc cacaggattg ccatacgctc aacaatgacg gcagcctgcg
    11281 ccagtcggtc gacacgctgc tgacgctgat tcatcagaag gagaaacacc atgcctgctt
    11341 gtgagcttcg cccggccacg cagtacgaca ccgacgcggt ttacgcgctg atttgcgagc
    11401 taaaacaggc ggagtttgac caccacgcgt ttcgcgtggg ctttaacgcc aatctgcgtg
    11461 acccaaacat gcgctaccat ctggcgctgc tggatggcga agttgtcggc atgatcggcc
    11521 tgcatttgca gtttcatctg catcatgtca actggattgg cgaaattcag gaactggtgg
    11581 tgatgccgca ggcgcgcggt ctgaacatcg gcagtaaatt actggcatgg gcagaagaag
    11641 aagcccgcca ggccggggcc gaaatgaccg agctttcgac caacgtgaag cgccacgacg
    11701 cacaccgttt ctatctgcgc gaaggctacg agcagagtca cttccgcttc accaaggtgc
    11761 tgtaacatga gcctgaccct cacgctcacc ggctccggcg gcgcacaggg cgttccggca
    11821 tggggctgcg agtgtgcggc ctgcgccaga gcgcggcgct cgccgcacta tcgccgccaa
    11881 ccgtgcagcg gcgtagtgaa gtttaacgag gctgtcaccc tgatcgacgc cggactgcac
    11941 gatctcgccg atcgctggtt gcccggatcg ttccagcagt ttttgctgac gcactaccat
    12001 atggatcacg ttcaggggct attcccgctg cgctggggcg tgggcgatcc gatcccggtc
    12061 tacggcccgc cggatgaaca gggctgcgac gatctgttta aacatccggg cctgcttgat
    12121 ttcagccaca cggtggaacc gtttgtggtg tttgatttgc aggggttaca ggtcacgccc
    12181 ctgccgctca accactcaaa actgaccttc ggttatctgc tggaaacggc gcacagccgg
    12241 gtggcgtggc tgtctgacac cgctggcttg ccggaaaaaa cgctgaaatt tttacgcaat
    12301 aatcagccgc aggtaatggt gattgattgc agccacccgc cgcgcgagga tgcaccgcgt
    12361 aatcactgtg atttaaatac cgtgcttgcg ctgaatcagg ttatccgctc gccgcgcgtg
    12421 gttctgaccc atatcagcca ccagtttgat gcgtggctga tggaaaacgc actgccgtca
    12481 gggtttgagg cggggtttga tgggatggag attggggtgg cgtgatgaga gggaatgtgc
    12541 gtgctggccc cctcccccta accctctccc cagaggggcg agggccgggg tgaggggcaa
    12601 tgcctgctcc atacccaaca tcatcgccca tactcatctt ccattctccg ctcttcatct
    12661 tctagctgcc gacgctcctg atcaagctgg cgctggcgat cgtccagctg cctgcggcga
    12721 tcttcaaact ggcggcggcg gtcgtcatat tgtctgcgcc gatcgtcgct cacttcacgc
    12781 tgccagccat cgtcgcgcga atcttcatag tctcgcccac ggtcagggtt ataagcgtcg
    12841 ttaatcgcct gctgaatatt gccaatggtg tcatcgataa tatcggcctg ggccggtacg
    12901 gtggtcagcg tgagcagggt gaaaaaaaag agcgtggagt ggcgtttcat aaactcggcc
    12961 tcgcaacaaa gttaatacaa ggtaggcaaa ggtgcctgtt acgagtagag gaggattctc
    13021 aattctcttg ccccagcgcc tgcgccaact gcgcacgggt aaagggtttg cgcaacagcg
    13081 ccacgtctgg cagcgcgggg ttatggctgg ggcgtaaatc ctgaccgctg atgagtagca
    13141 gcgtcaggtg cgggtaaagt ttgcgggccg cattgaccac ttctgcgccg ctcatgccgc
    13201 cggggagcat taaatcgctg ataaaaatat cgatttctgc tgatgccgcc agcagatcca
    13261 gcgcctgttc gccgctgctt gcttccagcg tcaaatagcc cagcaggtgc aactgttcgc
    13321 agatggtctg gcgcaccgcg gcttcatctt ccagcactaa caccagttta tcgccgctga
    13381 cagaagcctg ttctactgcc gctggcgata ggtttttcgc cgccgtcagc gcgcggggaa
    13441 gctgtaatct gaccgttgtc ccctgccccg gcgcgctttc aatttcaacg cgcccaccgg
    13501 actggcgcac aaaaccatag accatcgaca aacccagccc gctgccgctg cccgtctgtt
    13561 tggtggtaaa gaatggctca aatacctggc ttttcaccgc ctgcgacatg ccgcagccgt
    13621 gatcgatgac ttccagcgcc accatatcct gacgctgccc actgctgcga gtaacgcgct
    13681 gattccaggt acgaattttg atgattcccg tctgcccttc catcgcgtca cgggcgttca
    13741 tcaccaggtt gatgatggca ttttccagtt ggctgatgtc aatccacgcg ggccacgccg
    13801 gagactgcgc ttcaatctcc agcgccagcg ttgccggtaa ggagtggcgc attaattcgc
    13861 ttaactcttc cagcaatgtt ttcagttcaa cggcttgcgg atgtaaggac tgcttgcggg
    13921 aaaacgccag cagtcgttgt gtcagtagcg ccccacgttc ggcagcttgc agcgcccgct
    13981 gtattcgcgg cgcgtctggc gagtccggat tcaccagctc caggctgcca ataatcaccg
    14041 ccagcagatt attaaaatca tgggccagcc cgcccgtcag ttgcccgaca gctttcagtt
    14101 tctggctgtg taatagcgcc tcttccaacc cctgccgctc agtttgggca atctccatct
    14161 gcgtggtctt ctcttttagc agccgtgagg ttcgttcgag tgacgcggta ttgcgggcaa
    14221 aaacgttaaa ggcgcgcgcc agttcaccca actcatcacg ccgctgtaac gccggaaaag
    14281 tgacgtccgc ttcgccgtac gccaggcggc tcatggcgcg ggaaatcgcc gtcaggttag
    14341 tgccgaggtt gcgatagata taccagccgg caaacgcggt gatcaccacc gctaacaggg
    14401 cgaaaatcat gatcaccctc tcgccggagc gcaaatcctg gtaactttgc gccacgcgct
    14461 gaccggagag cgtcgccacc tgttcggcgt aacggttgat gtcgccattg aggatcgcca
    14521 ccagcgcttt gatgtgaaac atataccagc tgattgccag atcgctttgc tccagagctg
    14581 ccgagagtgg cgccagttta cgcagttcac gattaaaatc attgagtagc gtgttaacca
    14641 atggcgtatc gctgtgggtc ggcaacgcag acattacccc gaccagttga tgaataatcg
    14701 cctgcggcgc gggggtggca atagcggcaa caatcagtcg gttcatctcg ttaagcaaaa
    14761 tatcatcttg cgcgtgagtg actttttgca gatgccgcag ataacttaag ttttgatata
    14821 gcgaacttaa cagcgcgttg cgctccagat gccgcctttg cccgcgctcc agcataccgc
    14881 cgacgctggt ttgcagttcg ttgctacgcg tgatgattcg cgtcaccagc ccggcttcct
    14941 gctgcgctaa aggcgcgttc gccagttgtg caagcgagct ttttaatgcc cgctgtgtct
    15001 cctgcaaccg ctcggcttcg cctttatatt ccagcgctcc caccacctgc gacaatcgca
    15061 ccgccgccgt tgccacgttt gccgtgtcgc gcgccagatt caggctgccg gtcatatcat
    15121 ccaccgtttg ctgctgcacc agctcctgca tctggctggc atgacgaaag cccattaccg
    15181 ccacgccgct gaccatcaac gtcaccgcca ccaccagcaa attcaaaagc agcaatcgcc
    15241 cgcgcgcgct ggcaaaaaag tgacgtccgg ggtgcgccat atcacgctcc gtgaagaaaa
    15301 atcgcaatat gacaaatatg aacggcttct gacattttgc atttactttc ctgtgatgga
    15361 gtgctgatat cgacaacctc gcaggagtcc accatgaaca gcacgccagt tctggagatg
    15421 cgcaatattg ccaaagcctt cggcaaattt tatgcactca aaggggtgga tttgacggtc
    15481 taccctggcg agatccatgc cctgatgggg gaaaacggcg cgggaaaaag cacgctgatg
    15541 aaggtgctgg cgggggcgta taccgccacc agcggcgaga tcctcataga cggcaagccc
    15601 tttcacattc gcacgccaaa agatgcctta agcgccggta ttacgctgat ttatcaggag
    15661 atgcagctgg cgcccaattt gacggtggca gagaacattt tcctcggtag cgagctttcc
    15721 cacggcgggc tggtgcagcg taaagagatg ctggtgcagg cgcaaagagt gatcgaccgc
    15781 ctcggcgcgc agtttaacgc cagcgataag gtcatgacac tgaccattgc cgagcaacag
    15841 caggtcgaaa tcgcccgcgc gctacatcgc aacagccgca ttctggtgat ggacgaaccc
    15901 accgccgccc tctcctcccg cgaaactcac cgcctgtttg aactgattat gcggctgcgc
    15961 gatgaaggga tggcgattat ctacattagc caccgcatgg cggaagtgta tgagctttcc
    16021 gatcgcgtca gcgtgctgcg cgacgggcaa tacgttggca gcctgacccg cgataacctc
    16081 aatgccgggg agctggtgcg gatgatggtc ggcaggccac tgagcgatct gttcaataaa
    16141 gagcgcgata tcccgctcgg taaagcccgc ctgaatgttc accacctgac cgacggcggc
    16201 aaagtccagc cgagtagcct gctggtgcgt tccggcgaaa ttgttggcct cgccggactg
    16261 gtgggtgccg gacgttccga actggcgcag ttgatcttcg gcgtgcggaa agcgacaggc
    16321 ggaatgattg aagtcgatgg tgaaccggtg gtgatccact ccccgcgcga agccatcgat
    16381 cttggcattg gttttctcac cgaaaaccgc aaagaacaag gcttattcct tgaaatggca
    16441 gcagccgaaa acatcaccat ggcaaccctg gagcgcgatg cccgctgggg aatgctcaat
    16501 cgcaaaaaag cgcaaaccat ttccgatgac gccattaagt tgctcaacat tcgcgtgcct
    16561 cacgcccagg tacgcgcggg cgggctttcc ggcggcaatc agcaaaaact gttgatctcc
    16621 cgctgggtgg cgattggccc gcgcatttta ctgctcgatg aacccacccg cggcgtggac
    16681 gttggcgcca aaagcgagat ctaccggatc atgaacgaga tggcgcgcaa gggcgtggcg
    16741 atcctgatga tctccagcga actgccggag atagtgggaa tgagcgatcg cgtctatgtg
    16801 atgcgcgaag gcagcattgc cggtgagtta aacggcaaaa acatcaccca ggaaaacatt
    16861 atgaccttag cgactggcgt caacgacgcc cattcccagg cggtaacctc atgacgacct
    16921 ctgcaaaaac ctctgccaaa aaaatgctga tgagcgatct gatgcaaacg gtcggcatct
    16981 tgcccatttt gattctgatc gtggcggtat ttggctttat cgcgccaaac ttttttaccg
    17041 aaagcaacct gctgaatatt acccgccagg catcgatcaa cattgttctg gcggcgggga
    17101 tgaccttcat cattttgacc ggcgggatcg atctttccgt gggttcgatc ctcggcacca
    17161 cagcggtggc ggcgatggtc gtctccctta gccctgagtt cgccatgctg tcgatcccgg
    17221 cagcgctgat gctcggcctg ctgctggggc tgttcaacgg cgcgctggtg gcctttgccg
    17281 gattgccacc gtttatcgtc acgcttggca cctatacggc gctgcgcggg gcggcgtatc
    17341 tgctggcgga cggcaccacg gttatcaact ccaacattaa tttcgagtgg ataggcaata
    17401 actatctcgg gccggtcccg tggctggtgg tgattgccct cgcagtgatt gtgctgtgct
    17461 ggtttattct gcgccgcacc acgcttggcg ttcatatcta cgccgtcggc ggcaatatgc
    17521 aggcggcgcg tcttaccggc attaaggtct ggctggtgct gctgtttgtt tatggtatga
    17581 gcggcctgct ttctgggctt ggcgggatta tgagcgcctc acggctgtac agcgccaacg
    17641 gcaacttagg catgggttac gaactggatg ccattgcagc ggtgatcctc ggcggcacca
    17701 gttttgtcgg cggcatcggc accatcaccg gcacgctggt cggcgcgcta attatcgcca
    17761 cgctcaacaa tggcatgacc ttaatgggcg tctcttactt ctggcaactg gtgatcaaag
    17821 gggcggtgat catcattgcg gtgctgatcg acaaataccg tacccgacac catcaaagtg
    17881 cataaacaca tgaggaatac agcatgagat tgaagccctt agtgaccgcg ctctgtgcag
    17941 gcgcgctgct ggccgcagca ccgttcgccc aggcgaaaga actgaaatcg ataggcgtaa
    18001 cggtgggcga tctggcgaac ccgttcttcg tgcagatcac caaaggcgcg gagctggaag
    18061 cacgcaaact ggcaggcgat aacgtcaaag tgacgctggt ttccagcggc tacgatctgg
    18121 gtcagcaggt gtcgcagata gacaacttta tcgccgctaa cgtcgatatg attatcctca
    18181 acgctgccga ttcaaaaggg atcggcccag cggtgaaacg ggcgaaagat gccgggatcg
    18241 tagtcgtcgc ggttgacgta gcggcagaag gtgctgatgc caccatcacc tccgataaca
    18301 cccaggcggg cgaaatggcc tgtaaataca tcaccgatcg cctgaaaggc aaaggcaatg
    18361 tggtgatcat caacggcccg ccggtatccg ccgtgcaaaa ccgcgtcgaa ggatgccaga
    18421 ctgaatttaa acgccatcct gacatcaaag tgctctctga caaccagaac gcgaaaggca
    18481 gtcgtgaagg cgggctggag gtaatgacgg gactgctggc ggcaaatccg aagatcgacg
    18541 gcgtatttgc gattaacgat cccaccgcaa tcggtgccga tctggcagca aaacaggcgc
    18601 aacgtaacga gttctttatt gttggtgtgg acggaagccc ggacgccgaa gaggcgctca
    18661 agcgtgagaa ctcgttgttt gtcgctactc cggcgcaaga tccccaggtg atggcagcga
    18721 aagcggtgga aattggctac gacattttgc agggcaaacc tgcgccgaaa gagccggtgc
    18781 tgatcccggt gacgatgatc gataaaaaca acgtcggcaa ttacaaaggc tggacggtga
    18841 aataagtcca cacgccgggc ggcgcagctg cccggtcacc gctaaggaga acgctttcga
    18901 tgaaacgctc cgctataaac gacattctcg gccatacacg gcaattcttt tcgcaacatg
    18961 acgtacatct gccgcccttt gccagtttct cgcctgctca atggcagcag ctcgacaccg
    19021 ccgcgtggga ggaggttttt gatcttaaac tcggctggga tgttaccgct tttggtcgca
    19081 acaactttgc agcacacggg ctgacgctgt ttaccctacg taacggctca gcaaaaggaa
    19141 tgccgtatgt gaaatgttac gccgagaaga tcatgcatgt gcgcgatgcg caggtcacgc
    19201 caatgcattt tcactggcgt aagcgtgagg acattatcaa tcgcggcggc ggtaatttaa
    19261 ttgttgaact gtggaatgcc gatagtaacg agcaaacggc tgatagtgac attacggtgg
    19321 tgatcgacgg ctgccgccag aaacatactg caggcactca gttgcgcctg tctcccggcg
    19381 aaagtatctg cctgccgccc ggcctgtatc acagcttttg ggcggaaacc ggctttggcg
    19441 atgttctggt tggcgaagtc tcttctgtca atgacgacga ccacgataac cactttttac
    19501 agccactgga ccgctacaac ctgattgacg aagacgaacc tgcacagttg gtattgtgta
    19561 acgaataccg ccagttccgc tgaataagga taacgttatg ccattgattt ctctcgctga
    19621 agggctggca catgcccgcg aacatcacta ttcgttaggt gcgtttaatg tgctggactc
    19681 gcacttttta cgcgccttgt ttgctgctgc cagacaagaa cgttcgccct ttattatcaa
    19741 cattgccgaa gtacatttta aatatatctc gctggattcc ctggtcgaga cagtgaaatt
    19801 tgaagcggcg cgtcatgcca ttccggtggt gttaaatctc gatcacggac tgcattttga
    19861 atcggtggtg caagcgttgc ggctgggatt cagctcggta atgtttgatg gctccacgct
    19921 ggggtatgaa gaaaatgtcc gccagacgcg ggaagtggtg aaaatgtgcc atgccgttgg
    19981 cgtatcggta gaagctgaac tgggcgcggt gggtggcgac gaaggcggtg cactctatgg
    20041 ccatgccgat gaatccctgt ttaccgatcc gcaactggcg cgtgattttg tcgaccgtac
    20101 cggcattgac gcgttggccg tggcgattgg caatgcacac ggcaaatatc gcggcgaacc
    20161 gaaactcgat ttcccgcgcc tcgacgctat ccgccagcag gctgctatcc cgctggtatt
    20221 acacggcggc tcagggatta gcgatgccga tttccgccgc gccatcgaac tgggtatcca
    20281 caaaatcaat ttctacactg gcatgtcgca agcggcgctg gctgccgttg aggcccgaat
    20341 ggcgcaccgt cagccgatgt atgacgagtt tgccgagtta ctgctggcag tggaagccgc
    20401 catcagcgat acggttgcac aacagatgcg tatttttggc agtgcggggc aggcatgatg
    20461 gagcgtaaag ggattatcgc cgcaggcaat atgttggttg accacgtcca tcagattatc
    20521 cagtggccag agcgcggctg gctggcggag atcacccaca gcgaacgctc caccggcggt
    20581 gcgccgctta acgtcctgct gacgctggcg aaaatgcgca ccgggctgcc gttacaggcg
    20641 gttgggctaa tcggtcagga cagcgacggc gattacatca tggcgatgct tgagcaatat
    20701 cacgtcaatc gtcagcatgt gcagcgcact acctttgccc cgacgtcgat gtcgcaggtg
    20761 atgacagacc caagcgggca gcggacgttt ttccattcgc cgggtgcaaa cagacttttg
    20821 gatcttccgg cgttcgatca gctcgataac accatgaaga ttttccatct cggctattta
    20881 ctgttgctcg acagcctgga tatgccggac gatgtttacg gcacccgtag cgcacgcctg
    20941 ctggcgcaaa tgcgtgatac tggctttgaa acctcactcg acctggtctc ccgtaagggc
    21001 gatccgcgtt atcagccgct ggtgctgccc gcgttgcgcc atctggatta tctggtaatt
    21061 aacgaactgg aggccgggga gtttagcggg ctggaaattc ggttacccaa cggcgaaccg
    21121 catatcgcgc atattgctgc cgccgcgcgt gaactgttag atgcgggcgt gcgccagcgg
    21181 gtggtgatcc actgcccgga aggggcgtgg ggagagaccc cagaacaggg cggcgtgtgg
    21241 atcccttcgc aaaagttaga acaacgggaa attatcggca gcgtcggcgc gggcgatgca
    21301 ttttgtgccg gatttttata tggctgccat gaacgctgga cgcttaccga gagcatcaaa
    21361 ctggcacacg cctgcgcgcg ggcaagcctg ttatgcgcca atgccatcga cggggcgaaa
    21421 acgctggaag agctgcaaac agaaatgtcg gattaatgca cgtcggcagc gaagacatac
    21481 cccagaccgc gcagagtttt aatcaacatc ggctgatgcg ggttgagttc gattttgcgt
    21541 cgtaagcgca tgattaatac gtcgatggtg cgatcaaaca cctccacgct gtcgctgtgg
    21601 gtgaacgcca gcaactgctc gcggctcaat acccgacgcg cgttttgcgc cagagccagt
    21661 aacaagccat attcgccctg agttaactcg acatgttgct gaagcggatt gtgcaactgg
    21721 cagcgcatgg tatcgagata ccagccattg aatgacagcc cggcgctttt ctcgtcatta
    21781 accaccagcg caccaacccg tcgcaacacc gctttcagtc gtgcgaccac cacccgcggg
    21841 gtgaacggtt tgcagatgta atcatccgcg cccatttcca gcccgaccac cacatcagac
    21901 tcactgccca tgccggtcaa catcaccacc ggcaatgtcg gacgcagttt ctggatttgc
    21961 tgcaataccc tcaggccgtt ggtatcgggt agcatcatat ccagcaccac cagcgcaatg
    22021 tcaggatcgc cctcaatacg caatatggcc tcctgtccgg tatggcagac tgaaaccgta
    22081 aagacatgtt cacttaacac gtcctgcaac agtgcgcaga tagccgtgtc atcatccacc
    22141 accagcacga cgggtttcat ggttacactc cgcatcattc atctgattaa tcattgtgcg
    22201 tacttcttgt cgaagtgaaa gctgaaagta atataaataa
//
LOCUS       DAZFFG010000081        22156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFFG010000081 DAZFFG010000000
VERSION     DAZFFG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.081"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(79..792)
                     /locus_tag="ACHJ7J_003350"
     CDS             complement(79..792)
                     /locus_tag="ACHJ7J_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001541.1"
                     /translation="MNDSALLRNSSLFVAYMGCLGWGSAYFYGWGTSFYYGFPWWVVG
                     AGVDDVARSLFYAVTVIVIFLIGWGTGVVFFLGIKQRNNVQDLSFIRLFLAILLLLVS
                     PALEFSVIHRHVVLDALVLCVIAALIMSIFVRSGRRLILVKCFSEVSFIRHHLIECMM
                     AGFMIYFWGFSLIAGWYKPQFKNEYQMLHYESVWYYVLARYDDRLVLSTSYKSGSDKF
                     VILNTKNTDNFEINMVRVR"
     gene            1546..1770
                     /locus_tag="ACHJ7J_003351"
     CDS             1546..1770
                     /locus_tag="ACHJ7J_003351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001542.1"
                     /translation="MTKSQIGIVTLSEVETDKKTKTIILEMNEHMRESGEATSEFYIG
                     KVSVNHLLLPENNLRDFFVALKEDLKKFRH"
     gene            complement(2100..2930)
                     /locus_tag="ACHJ7J_003352"
     CDS             complement(2100..2930)
                     /locus_tag="ACHJ7J_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5001543.1"
                     /translation="MKLESSLKHFSPMGMHISNDVKGTSPERLTSTDVMAAIGTTSNR
                     ERFGLAAFFGKVGISKTDEQMAVQALARHAMEIAPKNVRKAAGGEFGWCMLVLAQFAF
                     AEYSRSAATSATCHSCNGTGLTTRKQVIRKVSYPCGKAPYWASRSRAVRPSDWEKWTK
                     VTEVVAVVCDVCKGKGVISARCRCGGKGDVLDRKATSERGVPVFKICERCSGNGFSTV
                     PSTAVYKVVLKRIPELHVRTWTRNWKPFLDSLVDICHQEERKADIAFQNVTSFGDDVN
                     "
     gene            complement(2973..3215)
                     /locus_tag="ACHJ7J_003353"
     CDS             complement(2973..3215)
                     /locus_tag="ACHJ7J_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001544.1"
                     /translation="MIFTPEQLRKQAQEMLRQAEQLEKTGVTKDAIRRDMVPALRELM
                     QAKHRAQKAMDELVDCVAELETKVGKFEKLVQEVLR"
     gene            complement(3199..4614)
                     /locus_tag="ACHJ7J_003354"
     CDS             complement(3199..4614)
                     /locus_tag="ACHJ7J_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5001545.1"
                     /translation="MISQKSPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFS
                     VRPYRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLAEALQKLRDAHTCEAAEDALKDAQNMMASLSTEKGIIQPIHIDDVL
                     PEVVDRVECRNKGLEKSRTLMTGIDELDAKTGGMEPGDLIFIAARPSMGKTELALDII
                     DKVTEQGRGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVSHFGDEDWARFIKGVEL
                     MTGRNIWMVDQANLTIDEICVTTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGE
                     ISKGLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIIMMLYRD
                     EVYNPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQFLSTPMRPSRPR
                     RYSNKRTDSSKIKQPDNDFYP"
     gene            complement(4611..5489)
                     /locus_tag="ACHJ7J_003355"
     CDS             complement(4611..5489)
                     /locus_tag="ACHJ7J_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5001546.1"
                     /translation="MMTFNLREQQTRLQARIDELRSEMAFAETGEKPWPYRSCLIREG
                     RGYCEKHGEYHTHILVWGDSNGEDREKISHCPHCLSAEINDVIMELSSLKAEELTDNA
                     GIALRFRDCEFENYQEINPDAARNLAACRRYAENWADVLENGTNLVLTGSCGTGKNHL
                     AVAMAKYVIRNYFASVEITDVMRLTRAVKNCWRNDSEKTADEVIEHYASLDLLIIDEV
                     GVQFGSAAEMTILQEIINARYESILPTILISNLSPKALWAYISPRIADRVTDGGRNLL
                     SFNWPSYRAHARGVAA"
     gene            complement(5500..6408)
                     /locus_tag="ACHJ7J_003356"
     CDS             complement(5500..6408)
                     /locus_tag="ACHJ7J_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5001547.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKSNFAEEGNPSPRTRETTRCGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGIKPETMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPGGGR
                     QRQIDILSGLGAMSDEFGKSSDNLTF"
     gene            complement(6395..6628)
                     /locus_tag="ACHJ7J_003357"
     CDS             complement(6395..6628)
                     /locus_tag="ACHJ7J_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001548.1"
                     /translation="MIRRIVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            complement(6625..7353)
                     /locus_tag="ACHJ7J_003358"
     CDS             complement(6625..7353)
                     /locus_tag="ACHJ7J_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO5001549.1"
                     /translation="MTTTTTHPFLKIETVNGKVVTTSLFEYLKLLRCAGVPGYISRVA
                     VNPATGLRIPKHHTGLTRRKRVFLSAHVYSMAAQMGASSEAPVFPCVSGNANPVWAAT
                     NHGFASVGGSCKAHTQGAVTMTTTTTHPFLKIETVNGKAVIFSLHVACHFKRMHQNIV
                     DKIEYLNCSREFFSRNFIPGTYHVYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCI
                     EAFHEAETSLNHTAFRRNQWEVRP"
     gene            complement(7399..7563)
                     /locus_tag="ACHJ7J_003359"
                     /pseudo
     CDS             complement(7399..7563)
                     /locus_tag="ACHJ7J_003359"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(7652..8044)
                     /locus_tag="ACHJ7J_003360"
     CDS             complement(7652..8044)
                     /locus_tag="ACHJ7J_003360"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO5001550.1"
                     /translation="MGRKARHLANKEIIKVLAERYPESFFVIPYKVVPLVHDIVHELF
                     ADLGHIPKEYKNSLRRAIYQYKSSVAYLQSVAFGEYRRNLAGNRIVRTSVSEREEARA
                     QLKERGIWTHRMELLFKVNMGRKEASNR"
     gene            complement(8108..8281)
                     /locus_tag="ACHJ7J_003361"
     CDS             complement(8108..8281)
                     /locus_tag="ACHJ7J_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5001551.1"
                     /translation="MQVLNRWFCDGRGRRVHVIRWESETQRVIYLRDGYPHECFSPLW
                     LFRRDFVECEAPA"
     gene            complement(8289..8507)
                     /locus_tag="ACHJ7J_003362"
     CDS             complement(8289..8507)
                     /locus_tag="ACHJ7J_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO5001552.1"
                     /translation="MMQTKTCVTGDVDLKPCPFCGNPEVQLIEVKYFLDGDDGYYIAC
                     TCCNANQIPDSKERAVHDWNQREGVGVE"
     gene            complement(8504..9364)
                     /locus_tag="ACHJ7J_003363"
     CDS             complement(8504..9364)
                     /locus_tag="ACHJ7J_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5001553.1"
                     /translation="MMINKQKLVNPENLPVIEWQGVRVVTTETLAAGYGTDEANIRKN
                     LSRNLERFEDGKHYFLLTGDALKAFKDRVTIGHSVGRNARSLTLWTELGSARMSKIVD
                     TDEAWSFFEKMEQAYFRKATPSSNTIPNFDDPIAAAEAWIEAKKSERLALGYAERQAR
                     YISKLESYLADGITPVQFCKQLNGVNTRQINAFLEERNWLYDDRPEAMYPRWRVKAYA
                     RDQYLTERSGQVEQENGEMREIFKPLLKKKGAAWLYRHYLNGELPMKKTWDGLFTHNT
                     ELANLLQENK"
     gene            9726..9983
                     /locus_tag="ACHJ7J_003364"
     CDS             9726..9983
                     /locus_tag="ACHJ7J_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001554.1"
                     /translation="MRGKIVHVEHRIGWIAIRDDRGAITIAEIIGGYDVEKGHIISGN
                     LHSLGSEVFHNLTTSEEMDVMVEYIGLTDNQAIAAIQRCGR"
     gene            complement(9952..10176)
                     /locus_tag="ACHJ7J_003365"
     CDS             complement(9952..10176)
                     /locus_tag="ACHJ7J_003365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001555.1"
                     /translation="MMGIDMQQLSAKKAQQLLEFVSNVEQAAKRGLEVNRELEFIPAE
                     KKISTKQCEWILKDCKLFRRAIYRIFGLQQ"
     gene            complement(10179..10445)
                     /locus_tag="ACHJ7J_003366"
     CDS             complement(10179..10445)
                     /locus_tag="ACHJ7J_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5001556.1"
                     /translation="MKGNDYDKLSALIAQNAIARNLGVTPQAVNQWFSKNTIPARFVL
                     RVCEVVAWKVTPHGLRPDLYPHPEDGIPDSLRKISNPSLVHTED"
     gene            10579..11268
                     /locus_tag="ACHJ7J_003367"
     CDS             10579..11268
                     /locus_tag="ACHJ7J_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5001557.1"
                     /translation="MKNVKNTENRIAAMLKAKGWTQAQLARKLGVSAQSVQYWTTGKT
                     FPRSDKLAHLSEISGYPQSWFLGEDSSPTFSSQEKHQTRTDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGLALLYIQSKVI
                     SSVPFNINRFG"
     gene            11414..11866
                     /locus_tag="ACHJ7J_003368"
     CDS             11414..11866
                     /locus_tag="ACHJ7J_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5001558.1"
                     /translation="MKTLKATPETTNFINCGCITLNNLELDSFALNIANLLSAVRTFH
                     LLDCARSKELGIEVMEFIHEYALSAASPAQQKQSFPKSWLVNLRTQRETCGLTTAELA
                     RLLDLDEDIIIQWESGEYEPTISMLIPLANVLGCDPMWLLTGSNAAKE"
     gene            11872..12072
                     /locus_tag="ACHJ7J_003369"
     CDS             11872..12072
                     /locus_tag="ACHJ7J_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001559.1"
                     /translation="MNSNIHDNFEGICLASDSFAINIHSLLCAAQVLQMSNNQNAKTL
                     GDEILSFACEYSKAAVKKELAQ"
     gene            12269..12457
                     /locus_tag="ACHJ7J_003370"
     CDS             12269..12457
                     /locus_tag="ACHJ7J_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001560.1"
                     /translation="MEEKRQNIAHEKVRVLLIIENGKVIYSRHLLDNEFACSMDTFLW
                     MAKRAGYTIIPPAKEQTL"
     gene            12454..13035
                     /locus_tag="ACHJ7J_003371"
     CDS             12454..13035
                     /locus_tag="ACHJ7J_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001561.1"
                     /translation="MNNFEFKPEATAHGIKIGNTTVDYVEAVQRLNDGEYDNPCWHGL
                     RIMQCIAEADDAGLLGRFSVDLKIAQWRWLYVATFISEEENKNGTIDIPNDNGTTDHA
                     VIYKGKHGCMSIYPGPLRIALQNHVEWGFIEKYGEAEGMGRVLFIYQKMLIADPDNGF
                     IVSAMGREGLELLLDEIIHDLNTHGMPEAPVTH"
     gene            13045..13323
                     /locus_tag="ACHJ7J_003372"
     CDS             13045..13323
                     /locus_tag="ACHJ7J_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001562.1"
                     /translation="MANQNKINVFQVESKHKTPVIKHVRRLTRLYTPEEFMAMPMIKK
                     FIRDNPGHVAIDKNNGEIMLSRELAEIYCNVNNGKKLKKTIRKISERS"
     gene            13339..13620
                     /locus_tag="ACHJ7J_003373"
     CDS             13339..13620
                     /locus_tag="ACHJ7J_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO5001563.1"
                     /translation="MTVSPTIKIDTVMNTGLAVIGWYYILFRAGQMLTSVFLKQWTKR
                     CKQEQRQKALEAFYDAFELSRIEPGTTARIATKGDLMIVMFRQEKTTSE"
     gene            13613..13753
                     /locus_tag="ACHJ7J_003374"
                     /pseudo
     CDS             13613..13753
                     /locus_tag="ACHJ7J_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            13943..14365
                     /locus_tag="ACHJ7J_003375"
     CDS             13943..14365
                     /locus_tag="ACHJ7J_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001564.1"
                     /translation="MNDKELIKEIKERIGSLDVRDNIERRAYEIALASLEADPVAWMH
                     VNNGIGIPAITRSKEVAESWLSNGWYVQQLYIAKPVPVIPDEVLSAIRNVARIRANFA
                     DFDGDRRGIGDCLDEAEQELIITINKYACHITAEQRRR"
     gene            14458..14700
                     /locus_tag="ACHJ7J_003376"
     CDS             14458..14700
                     /locus_tag="ACHJ7J_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5001565.1"
                     /translation="MFALIQRGQIYTDSTGYPVKIIRSTDHSVFFRRMDGNTGRVRIG
                     EFNNLFEHIDHQEYHKILADTEQEAHLKKLRSLKRK"
     gene            14704..14855
                     /locus_tag="ACHJ7J_003377"
                     /pseudo
     CDS             14704..14855
                     /locus_tag="ACHJ7J_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14864..15073
                     /locus_tag="ACHJ7J_003378"
     CDS             14864..15073
                     /locus_tag="ACHJ7J_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5001566.1"
                     /translation="MGLDCVPISTYCRDAGETIDAVNKRIQRGIWKEGVHVLKVDGVK
                     ERWVDLMEITKWARKNKDHYLSLEE"
     gene            15028..16200
                     /locus_tag="ACHJ7J_003379"
     CDS             15028..16200
                     /locus_tag="ACHJ7J_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5001567.1"
                     /translation="MGKKEQGSLSLPRGVTIRQHKTGSTLIITFTYKGVLCREPLSRM
                     EVNVRGVKYAERLLGEIKNQIIDGTFEYAKYFPNSQKLKLFGGVKKNKNIKSYLDEYL
                     IICQNRNLSPSTIKGYEKCLSALSALHKFHVSELTPAVLKNWIASRKTKLKTIRNNLS
                     FLRSAIDEAVTDGLLTINPVTLVSASRYHVLDNTPGNDDYEVDPFTPAETAAIYDACH
                     YPEWQNLFRFAFNTGLRSSELCALRWTDIDFISNTAHVQTASVVGVIKSTKTKAGTRK
                     VQLNNEALAALQAQKPYTALKSDFIFSDPKTRAPWANADAIRKKAWVPTLKKAGVRYR
                     NPYQTRHTFATRHISQGVNLFWLAGQMGHKGPEMLFRHYGSYLAEYDGKTAISATP"
     gene            16563..17804
                     /gene="intA"
                     /locus_tag="ACHJ7J_003380"
     CDS             16563..17804
                     /gene="intA"
                     /locus_tag="ACHJ7J_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO5001568.1"
                     /translation="MARKTKPLTDTEIKAAKPKDTDYQLYDGDGLTLLIKSSGSKLWQ
                     FRYHRPLTKQRTKQSFGAYPAVSLSDARKLRAESRVLLAKDIDPQEHQKEKVRNSQEA
                     KTNTFLLVAERWWNVKKASVTEDYADDIWRSLERDVFPAIGDISVTEIKAHTLVKAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LPQLMQTMRTASISMSTRCLFMWQLLTITRPAEAAEARWDEIDFNAKEWKIPAARMKM
                     NRDHTVPLSDEAISILEMMKSLSGGREFIFPSRIKPTQPMNSQTVNAALKRAGLGGVL
                     VSHGLRSIASTALNEQGFPPDVIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWAAF
                     VMTADRGSVIEGGIKGIRLVG"
     gene            complement(17814..18240)
                     /locus_tag="ACHJ7J_003381"
                     /pseudo
     CDS             complement(17814..18240)
                     /locus_tag="ACHJ7J_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_449149.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(18516..19082)
                     /locus_tag="ACHJ7J_003382"
     CDS             complement(18516..19082)
                     /locus_tag="ACHJ7J_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020303896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001569.1"
                     /translation="MDSVMYEERFERWLKVSIGLARFDPFIPNLVQSLGKMDATLCEM
                     DVSLVEKYKQGGNANEDYKLIQGHLTHSYLWVLGSYEVIRTLTQSIKENKSDDPAEVL
                     ERFQNAKKRFARLRIPLAKFEAAKAHNKTDYKIAYPGFDYQVGIAWQVSDDVVISRQE
                     LSDLFLETLEFTRVQKLRRSLANHDSLS"
     gene            complement(19909..20631)
                     /locus_tag="ACHJ7J_003383"
     CDS             complement(19909..20631)
                     /locus_tag="ACHJ7J_003383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001570.1"
                     /translation="MKNKDFDVAFEELLDEFERCVKKVKDVSHFKQNSAERKIRKLEE
                     DALTVITDYRNGKIQADKYTYRELTIEEYVSSMESAARWQGEPSKFLEAAFVSHKWGM
                     SQYKLGHKAEGRKHALIALKLMYMWTGANWALEMVAAKEQSDKIKREAASLGGKNKSQ
                     NYRRVKDEVIRLLKKNMPENGWKSKVAAINSLEKEITKFIEKDTSKNHDWTSWEKLLR
                     TISDWSRNDIELKEIFSEVVKR"
     gene            20756..20953
                     /locus_tag="ACHJ7J_003384"
     CDS             20756..20953
                     /locus_tag="ACHJ7J_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004114618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5001571.1"
                     /translation="MPTQTIKILRLPEVVKKIGIKRSTIYDWLNPKSPRYDKTFPKQK
                     RLGRQSVGWLESDLDEWLSPR"
     gene            21117..22001
                     /locus_tag="ACHJ7J_003385"
     CDS             21117..22001
                     /locus_tag="ACHJ7J_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5001572.1"
                     /translation="MSDSFERGINVLKQPLSILPEHISDLVLEHIRQSIHYEPVIGIM
                     GKTGAGKSSLCNALFEHPLSPVSHAEGCTRKPLHFTLNAGGRSLTLVDLPGAGESLKH
                     DEIYRQMYQEQLPKLDLILWVMKADDRACTTDEEFHRFLLDCGVSTDSIVFVINQADK
                     AEPSLEWDREKRSPSVEQLMTLTARSAAVSRQFFTPYPVHAVSAKTGYNMSRIVETMI
                     FALPPEATSGVFRQIRKEHRTKESETKARKDFADLLGELLSRILEAIPLPNVIRNGLG
                     VLREGLENAATAIWHWFF"
BASE COUNT         6339 a         5164 c         4737 g         5916 t
ORIGIN      
        1 tgcatcttat tttatataat taaccactca tctcacataa aatcgacaca ttaacatact
       61 attctttaaa atattatttc aacgcactct aaccatgtta atttcaaaat tatcagtatt
      121 cttagtatta agtataacga atttgtcact cccactttta tatgatgttg acaaaacaag
      181 ccgatcatca tagcgagcaa gaacataata ccagacactc tcatagtgta gcatctgata
      241 ttcattctta aactgaggct tataccaacc ggcaataagt gaaaatcccc agaaatagat
      301 cataaatccg gccatcatac actcaatcaa gtgatgacga ataaaagata cttccgaaaa
      361 acatttgact aaaataagtc ttcttcctga cctgacaaaa atcgacatga ttaatgcagc
      421 aataacacat aaaaccagtg catctagcac aacatgccga tgaattacag aaaactccag
      481 agcaggagaa accaaaagca gcaatatcgc cagaaaaagt ctgataaaac tcagatcctg
      541 cacattattt ctttgtttga tccccaagaa aaaaacaaca ccagtacccc atccaatcag
      601 gaatataaca atgactgtaa cagcataaaa aaggcttctg gccacatcat ctacgccagc
      661 accaacaacc caccatggaa aaccatagta aaaggaagtt ccccagccat agaagtaagc
      721 acttccccac ccaagacagc ccatataagc aacaaaaagt gaagagtttc tgagcagagc
      781 actgtcattc ataaccaatc ctctaacaat tcaagaacat taacttgttt tacataacaa
      841 ataaaatcct atgtagtcaa gagacctcat gccaagagaa actcattaca caatcgccat
      901 caaatttaat gttctgtgtc gttttcgagc ctaaaaaatc agcctattgg caggcttaag
      961 atgtgtggcg aagtaaccac tattcaccaa gtattcggta gttaaataac ttgtggagtt
     1021 ctcgttttta taccgaaaaa ataaacgctc atccttcaaa tagagcgatt catgattttt
     1081 ctcagaactg aaacggcatc gagaaacttg aaaaccggga ggaaattata tccagaccaa
     1141 attttgttac cgctacagac aaaggagttc aacaacttga ggagataatt ggttactaca
     1201 aatttaagga agaaatccac tgtggtctaa atggctgcaa ccaaccacat caaatgggtt
     1261 atattgtaaa aacatctgac agcatttaaa ctaacaccgg aaataaatgt ggtaaaaact
     1321 aatctggtgt ggaatttggt gaaaacgttc ttagtttcaa taaatttatg caactcgaaa
     1381 ccaatagaga aattattagt accgcaaaaa gtaaatgtga agcgtggcaa aaacattgaa
     1441 acaatacgaa agatcaaacc tacaatagat tatctatctt ctattataga aaatagtgta
     1501 aattccaatt actcctgaag acttggtgct acataaattc gttttttgac caagagccag
     1561 ataggtattg taaccctttc tgaagtggaa actgataaga agaccaaaac aattatatta
     1621 gaaatgaatg aacacatgcg cgagtctgga gaagcaacaa gtgaattcta cataggaaaa
     1681 gtatcagtta atcatcttct tttaccagaa aacaatcttc gtgatttttt tgttgcgctc
     1741 aaagaagatc taaaaaaatt caggcattga actacaaaca gccccaagac cagaaatttc
     1801 tgattttttc aaaattgtca gttcaatcaa agaacgaatc aaacgattaa aaaactgaaa
     1861 catgaagcca gtaaattcct aacttaaaaa atattgtccc atcgaaaaca aaataaaata
     1921 ctcatcgaca gctgatgaac ttgaattcca taatttcgaa cgcttttcaa acggactgaa
     1981 atgatagaag ccccaaaaag gggcttctat caaaatcatt tacgtattat tccgcatttt
     2041 cataagcata catgacagat tcggacaaaa tcaagtgcta tgtctcaaaa agcctaagtt
     2101 tagttcacat catcaccaaa acttgtcaca ttctgaaaag caatatcggc cttacgctct
     2161 tcctggtggc aaatgtcaac cagagaatca agaaacggct tccaattacg cgtccatgtc
     2221 ctgacgtgta gctcgggtat ccgcttcagt acaactttat atactgcggt agacggtacc
     2281 gtggaaaatc catttccgct gcaacgttcg cagattttaa acactggcac accacgctcg
     2341 cttgtggctt tgcggtcgag tacatctccc tttccaccac aacgacacct ggcacttatc
     2401 actcccttcc ctttacatac atcgcaaacg actgctacaa cttccgttac ctttgtccat
     2461 ttctcccagt ctgacggtcg aacagcgcgg gatctactgg cccaatatgg cgctttacca
     2521 catgggtatg aaaccttacg aatgacctgc ttacgggttg ttagtccggt accattgcaa
     2581 ctatggcatg tcgcgctggt agccgccgat cgtgaatact cagcaaaggc aaactgcgcc
     2641 agtaccagca tacaccagcc aaattcacca ccagctgctt tacgcacatt cttcggtgca
     2701 atttccatcg cgtgacgcgc cagcgcctgg actgccatct gttcatcagt cttgctaata
     2761 ccgaccttcc cgaagaaggc cgccaggcca aaccgctcac gattgctggt ggtaccaata
     2821 gccgccataa catcagtact agtgagacgc tctggcgaag ttccttttac atcgttgctg
     2881 atatgcatcc ccatagggct gaagtgtttt aaagatgact caagtttcat tgttcatcct
     2941 ccatagttct ggaaccatct gaatatcacg catcagcgca gtacctcctg caccagtttt
     3001 tcaaactttc caactttagt ttccagctct gccacacaat ccaccagctc atccattgct
     3061 ttttgtgcgc ggtgtttcgc ctgcatcagt tcacgtaacg cgggcaccat atcccgacga
     3121 atggcatctt ttgttacgcc cgttttttcc agttgttccg cctgtcgcaa catttcctgt
     3181 gcctgtttac gtaattgttc aggggtaaaa atcattatct ggttgtttaa tcttactgct
     3241 gtcagttcgt ttgttactgt atctgcgcgg cctggacggc cgcattggtg tggaaagaaa
     3301 ctgtgcgctt tcctggtcta caggcagaaa atgtccgtta taaaaacgtc ggtaaatcgt
     3361 ccccagagaa ccattacgtt gcttcgtgat attaatttct gcgatgcccc tggcctgcgt
     3421 atccgggttg tacacttcat ccctgtaaag catcataatg atgtcagcat ccgcctctat
     3481 ttctccggaa tttttcaggt ctgagttcat gggacgttta tttggtctgg actccacacc
     3541 gcgggagagc tgactcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     3601 cccctttgag atttcaccca cggcaagatc atgacgcccc gtggttcggg tttttatcag
     3661 cccgagataa tcaaccacca ccagtgccgt ttccggatgt ttaatcaaat ggtgtttcgt
     3721 tgttacgcat atctcgtcaa tggtcaggtt cgcctggtcc accatccaga tattgcgtcc
     3781 ggtcatcagc tccaccccct taatgaaacg cgcccagtct tcgtcaccaa agtgagaaac
     3841 agatttcagg cgcgatacag gcattcctcc ggcagcagac accatgcgtt caccgatctg
     3901 aatgttcgcc atttccattg tgaacagaag aacaccgcgc ccctgttcag tcactttgtc
     3961 gatgatatcc agcgccagtt ctgttttccc catcgaaggg cgcgccgcga tgaatatcag
     4021 gtctcctggc tccatgccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     4081 cctggatttc tccagcccct tattccggca ttcaacacgg tcaaccacct ccggaagcac
     4141 atcatcaata tgtatcggct gaataatgcc cttttccgtc gacaatgagg ccatcatgtt
     4201 ttgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcgc gtaatttctg
     4261 caatgcttcg gccagtgttt tttctgcatc acgcagtgcg gcattgcgtc gcagagctgc
     4321 aacatagtgt tccagagatg acttcaccca ggttttacgc ccggtatccg taatcaccgg
     4381 ggcaagttcc ggcatctcat tgcacaacag cacaggatca atcacgccgg acacacgggc
     4441 ctgtctgcag atgcctgtgt agatatcccg gtacggtcgt acagaaaaaa cgtccgccgg
     4501 tagtgtggcc agaatatcca tcacttccgg atctgcccca cgcaaaaaga atgcgccaat
     4561 aacagcacct tccagatcat cgttacgcca gaccgggctt ttctggctga tcatgctgcc
     4621 acacctctgg catgtgcacg gtagctgggc cagttaaagg acaacaggtt acggccacca
     4681 tccgtaaccc tgtcagcaat acgtggactg atgtatgccc acaacgcctt cggtgaaaga
     4741 ttgctgatca ggatggttgg cagaatgctt tcgtaccggg cattgataat ttcctgaaga
     4801 atggtcattt ccgccgcgct accaaactgg acacccactt cgtcgataat cagcagatcc
     4861 agtgatgcat aatgctcaat cacctcatcc gcagtttttt cgctgtcatt ccgccagcag
     4921 tttttcacag cccgggtcag gcgcatcacg tcggtgatct caacactggc gaaatagttg
     4981 cggatgacat attttgccat tgcaaccgcc aggtgatttt tcccggtgcc acaactgcca
     5041 gtcagcacga gatttgtgcc gttctccaga acatcagccc agttttcggc atagcgacga
     5101 caggcagcaa gatttctggc tgcgtcagga ttaatctcct gatagttttc aaactcacag
     5161 tcgcgaaaac gcagtgcaat tccggcgtta tcagtcagct cttccgcctt cagggaggac
     5221 aactccataa tcacatcgtt gatttcagca ctcaggcagt gagggcagtg tgaaattttc
     5281 tctctgtcct cgccattact atccccccac accagaatat gcgtgtgata ttcgccatgt
     5341 ttttcgcaat atccacggcc ttcacgtatc aggcaggagc gataaggcca cggtttttcc
     5401 ccggtctccg caaacgccat ctcagaccgt aactcatcaa tccgtgcctg tagtcttgtc
     5461 tgctgttcac gcaggttaaa cgtcatcatc gctgtcacct cagaatgtta agttgtcact
     5521 ggatttaccg aattcgtcag acatggcacc aagaccagac aggatatcta tctgtcgctg
     5581 tcgcccacct ccgggagcgg ctggctgttg ccagaaatct tcgaagtgac gatcgggtcc
     5641 aaagaacgtc gcagcctgct tcacgaactg tgtgccggta tttcctgtag cacgtaccca
     5701 ggcggcatac ctcttcacgc catcaagcat ggtctccggt tttattccct ccctgatacg
     5761 agctttccag gctttgaagg ctgccgactt tgaattacca ccagcacgtt tgggatattc
     5821 ctgccaggcc tgttcaaatt ccggtgaata ttcctgtcgg gcagaacgcg ctggtgcaga
     5881 cgcgtcagcg gatgcgccaa tagtgttttt actctctgta gtattctctg aagtaatctc
     5941 tgttgtattc tctgtaagat cgaaattggt tttcccttct ccgcagcgag tggtttcccg
     6001 tgtccgcggt gaaggatttc cctcctccgc gaaattggat tttacagttt cccgaaaacg
     6061 ggtttcccca tttctggaaa actgattgtt ttcattgata atttcattaa ggcgctcaca
     6121 atctatacgg tagaacattt tgtgctcaag acgcttgttg gtttcaacca aaatgcctct
     6181 tgacacaaga tgcttacgcg ctacagcctg ttgttcaaat gtaagtccgg tttcgtgttg
     6241 tatctcttca cgcgttttat gtacgccttc cgctgcatgt gctttatcct gccagtaaaa
     6301 aatctgacca aagaaaataa cagcgtgcgg acttcccatg tatttaacga gcccagggta
     6361 ataagcaacc ggatgtccaa aatcgagtag aagatcagac ggacgcatag ccacctccca
     6421 ggcgtttaaa catttttccg gactgaaacg ccaccagcgg ataactcagg gtatgagtac
     6481 gtccctgaac ctggcagaca accttctggc tttctgtatt gaccaggcaa acccgcagaa
     6541 cgtggccgtt gctggtggtg aaccactgcc ccacacgggg gcaacggttg tatcggtgat
     6601 acagggaatt aacgatgcgg cgaatcatgg gcgcacctcc cattgattac ggcggaaagc
     6661 agtgtgattc agactggttt cagcctcatg gaatgcttca atgcagctct cgtagtaccg
     6721 cattgtgcgc agacttaacc caagctgaag catcatcagc ccatcaaggg tgatgtaata
     6781 accacgcagg gaatcgccat acacgtggta agtacccgga atgaaattac gggagaagaa
     6841 ttcacgtgag cagtttagat actcgatttt gtcgacgatg ttctggtgca tgcgcttaaa
     6901 gtggcaggca acatgcaggg agaaaataac ggccttgccg ttgacggtct caattttgag
     6961 gaatgggtga gtagtggtgg tagtcatggt gacagcccct tgtgtgtgtg ctttacagct
     7021 accacccacg gatgcgaaac catgattggt ggcagcccag acaggattcg cattaccgga
     7081 cacacaaggg aaaaccggcg cttccgaaga agctcccatc tgagccgcca ttgaatagac
     7141 gtgcgcagac aaaaaaacac gcttacggcg tgtcagccct gtgtgatgtt tcgggatgcg
     7201 aagcccggtt gctggattta cagcaacgcg ggaaatataa cccggaacac cagcacagcg
     7261 caatagtttg agatattcga aaagagatgt agtaacaacc ttaccgttga cggtctcaat
     7321 tttgaggaat gggtgagtag tggtggtagt catggtgaca gccccggtag tctgtttttt
     7381 agaaaactca ccaccaaggc tttccacgac cttataggtg gtgagacgta caggggtgga
     7441 aataccggag actaccgaac ccggcccgac cgaagtcggc cctgtacgcc ccaccataat
     7501 ttgggcgtag caatgctcat gacaaaaaaa aaccgcatga gcgcggttat gctcagtaat
     7561 caatttcggg tttccacgcc cggcacccgc tttataaggt gcagggacag tgtaacgtcc
     7621 agaaattgag gaatcaatgt tttgataaaa ttcagcgatt agaggcttct tttctcccca
     7681 tattaacttt gaacaaaagc tccatgcgat gagtccagat accccgttct ttgagttgtg
     7741 ctctggcttc ttcgcgctca gatacggaag ttctgacaat acgatttcca gccagattgc
     7801 ggcgatattc tccaaacgcg actgactgaa ggtatgcaac ggagcttttg tactgataga
     7861 tggctcttcg aaggctgttt ttatattcct taggtatatg tccgagatcg gcaaaaagct
     7921 catgaacgat atcgtgaaca agcggaacaa ctttgtatgg aataacaaag aagctttctg
     7981 gatagcgttc agccaatact ttaatgattt ctttatttgc aagatgcctt gcttttcttc
     8041 ccatatacca ttctccatta ctccagatat cagaatgata tatccagatt ttttaagcag
     8101 ttcaagctta tgcaggtgct tcacactcaa caaaatcacg acgaaacaac cacaacgggc
     8161 tgaaacattc gtgcggatag ccgtcacgca ggtaaatgac tcgctgcgtt tcagactccc
     8221 agcgtataac gtggacacga cgcccccttc cgtcacagaa ccagcgattg agtacttgca
     8281 tttggatttc attccacacc taccccttca cgctgattcc agtcatgaac agcacgttct
     8341 ttcgaatcag gaatctggtt tgcgttacag caagtacatg cgatgtaata accatcatcg
     8401 ccatccagaa aatatttcac ctcaatgagt tgtacttccg gatttccgca gaacgggcaa
     8461 ggtttcagat cgacatcacc agttacacat gtcttggttt gcatcatttg ttctcctgta
     8521 aaagattcgc gagctcagtg ttatgggtaa acagcccatc ccatgttttc ttcatcggta
     8581 gttcgccgtt cagataatgg cgatataacc aggcggcacc ttttttcttc aggagtggtt
     8641 tgaaaatctc gcgcatttcc ccgttttcct gctcaacctg cccagaacgc tcagtcaggt
     8701 attggtcacg tgcatacgct tttacacgcc agcgcggata catagcctca ggccggtcgt
     8761 catacagcca gttacgctct tcaaggaacg cattaatctg acgagtgttt acaccgttga
     8821 gttgtttgca gaactgcaca ggagtgatgc catcggcgag atagctttcg agtttgctga
     8881 tgtagcgagc ctgacgctct gcatacccga gagcaaggcg ttcggatttt ttggcttcaa
     8941 tccacgcttc agcagcagcg atcggatcgt caaagtttgg aatagtgttt gaagaaggcg
     9001 ttgctttacg aaaatacgcc tgctccattt tttcgaagaa cgaccaggcc tcgtctgtat
     9061 cgacgatttt tgacatgcgg gcagagccga gttctgtcca gagagtgagt gaacgcgcat
     9121 ttcttccaac agagtgacca atagtcactc tgtccttaaa tgctttaaga gcatcaccag
     9181 ttaaaagaaa ataatgtttc ccatcttcaa agcgctcaag attgcgcgac agatttttac
     9241 ggatattagc ttcatccgtg ccatatcccg ccgccagcgt ttcagtggta actacgcgta
     9301 ccccctgcca ctcaatcact ggcaggtttt cagggttgac taatttttgt ttattgatca
     9361 tcattaaaac cctcactact tagaacaagt cgggttggcg attgccattg ctgcccgact
     9421 ttttctttac cttccgttct gcaactgttg tctggcctag tgcccattgc cgcgcacgaa
     9481 acaagcaatc atcaaacatc gctcctttgc ggctggcctg cgagctacgg cggtagtaat
     9541 ccagtccccg gcatgccccc ccctggcaac aatttcaggg aagccctccg ccaccagctt
     9601 ttccgttatg tgtttgcaga taaattcttc tggagtcatg gttcatctct tcaatttgcc
     9661 tgtcttttca ccacttcagg ctcggtggta tcctctaaag tccctattac ttgagaggtt
     9721 ttgatatgag aggaaaaatt gttcatgtag agcatcgcat tggctggatt gctatccgtg
     9781 acgatcgtgg tgccatcact atcgctgaaa tcattggcgg atatgatgtt gaaaaagggc
     9841 acatcatctc cggcaatctc cattcactag gtagcgaggt attccataac ctaacaacat
     9901 cagaagaaat ggatgttatg gtcgaataca tcggcctaac cgataatcag gctattgctg
     9961 caatccaaag atgcggtaga tagctctacg aaaaagcttg caatccttca gaatccactc
    10021 gcattgtttt gtcgagatct ttttttcagc gggtataaat tcaagctcgc gatttacctc
    10081 caggccgcgc tttgcggcct gttcaacgtt gctcacaaat tccaaaagct gctgtgcctt
    10141 tttggcggat aattgctgca tatctattcc catcacttct aatcctctgt gtgcactaag
    10201 cttggatttg aaattttgcg taacgaatca ggaattccat cttcagggtg aggataaaga
    10261 tctggcctta agccatgtgg cgtgaccttc catgcaacta cttcacatac gcgtaaaacg
    10321 aaacgagcag gaattgtgtt ttttgaaaac cactgattca ccgcttgcgg cgtcacacca
    10381 agatttcgag ctatggcatt ttgcgcaatt aatgcactaa gtttgtcgta atcatttcct
    10441 ttcataacaa agcaccaata ttaactttat agatcaagaa tacatcaagt ttaaattaac
    10501 atgcaagttg caaaaggatc gaatacacta aaatcaagta aagatttatc cttgtaaaga
    10561 aacccacagg atttggtcat gaagaacgtc aaaaacacgg aaaatcgaat agccgcaatg
    10621 ctgaaagcaa aaggatggac tcaggctcaa ctggcccgca agttaggtgt gagtgcgcaa
    10681 tcagtgcagt actggacaac aggaaaaaca tttccacgga gtgataagct cgctcattta
    10741 tcagagatta gcggttatcc acaatcctgg ttcttaggtg aagactcctc accaaccttt
    10801 tcctcgcaag agaaacacca gacaagaaca gatagcgtcg tgtttaatgt ccttgatgtt
    10861 gagtttagtt gcggtgatgg aactcatgtc cgtggtgact tgatagatgt agtgcgctca
    10921 atagaacttg atcctgaata tgcccgacgc cttgttggaa atcgggcatt caaaaatata
    10981 gaaataggta acgccagagg agacagtatg gctcccacaa tctcacctgg cgaccttctt
    11041 tttcttgata agacagtaac ttattttgat ggcgatggca tttatgcatt ttgttttgat
    11101 ggagaatgct acgtgaaaag acttcaaaaa attggaagca aaatcatggt gttatctgat
    11161 aaccccaatt atcaaccatg gagtatagaa aaagaggggt tagctctgct ttatatccag
    11221 tctaaagtga tctcatcagt accattcaat ataaacagat ttggttagtc tttgatttta
    11281 acgggctttg cccgtttttt tctgcctaaa acacacgata tcaatttttt cttgacagcc
    11341 tatttctcaa agcataatat cgcaccatca attataactt gattaagttc aatttaaaat
    11401 tgttggtgga tatatgaaga cactaaaagc aactccagaa acaactaatt ttatcaactg
    11461 tggctgtatt acgcttaata acttagaact tgattccttt gcattaaata ttgcgaattt
    11521 gctaagtgct gtacgcacat tccatcttct ggattgtgct cgctcaaagg aactgggcat
    11581 tgaggtaatg gaatttatcc atgaatatgc cctatctgcg gcttctcctg cacaacaaaa
    11641 acaatccttc cccaaaagct ggctggttaa ccttcgcacc caacgcgaaa cctgcggctt
    11701 aacaaccgcc gaactcgcca gactcctcga tctcgatgaa gatatcatca tccagtggga
    11761 aagcggagag tacgaaccta ctatcagtat gcttatcccg ctggcaaatg ttcttggttg
    11821 cgacccgatg tggttattga ctggtagcaa tgctgcaaag gagtaacagt cgtgaatagt
    11881 aatatccatg acaatttcga aggtatttgt ctggcatcgg attcatttgc aataaacatt
    11941 catagtctgt tgtgcgctgc gcaagtttta cagatgtcaa ataatcagaa tgcaaaaaca
    12001 ttaggtgatg aaatcctgtc ttttgcatgt gaatattcga aagccgctgt caaaaaagaa
    12061 ttagcgcaat aacaatgaat attccatgaa tgtttattac ggttttatcg ccgtggattg
    12121 ttgcaacctt tattcgcagg agattatgtt atgactttcc tgaaacataa gacatcgtat
    12181 aaaactgcct gcctcattgc acaacatgga gattcttatc ttcatatagc caacctgtat
    12241 ttgcgcaaag catatgggag ataaatcaat ggaagaaaaa cgacagaaca tagcacatga
    12301 aaaagtaaga gtattgctaa tcattgaaaa tgggaaagtg atttactcaa gacatctgct
    12361 ggataatgaa ttcgcctgta gcatggatac atttctgtgg atggccaaaa gagctggcta
    12421 cacgattatt ccaccagcaa aggaacaaac attatgaaca attttgaatt caaaccagaa
    12481 gcgacagctc atggcataaa aattggcaat acaaccgttg attatgttga ggccgtacag
    12541 cggcttaatg atggtgaata cgacaatcca tgctggcacg gtttaagaat catgcaatgt
    12601 attgcagaag ccgatgatgc cggattactg ggaagatttt cagtcgatct gaagatcgct
    12661 caatggcgct ggctgtatgt ggcgacgttt atcagtgaag aggaaaacaa gaacggtacc
    12721 attgatatcc ctaacgataa cggaaccaca gatcacgcag ttatttataa gggaaagcat
    12781 ggttgtatga gtatataccc ggggccactt cgcattgctc tgcaaaacca tgtcgaatgg
    12841 ggattcattg aaaaatatgg cgaagctgaa ggtatgggac gagttttgtt tatctatcaa
    12901 aaaatgctca tcgcagatcc tgataatgga ttcattgtct ctgctatggg gcgcgaaggg
    12961 cttgaattac ttctggatga aattattcac gatctgaata ctcatggtat gccagaagca
    13021 ccagtgacac attaaagatt aaatatggca aatcagaata aaattaatgt atttcaggtt
    13081 gaatcaaaac ataaaacacc agtaataaaa cacgttcgcc gccttacacg cctctataca
    13141 ccagaagagt ttatggcaat gccaatgata aagaaattta tccgtgataa tccaggtcac
    13201 gttgctatag ataaaaacaa cggagaaata atgttatccc gcgagcttgc cgaaatttac
    13261 tgtaacgtga ataacggtaa aaagttaaaa aagacaatcc gaaagatatc agaaagatca
    13321 taaaggtaag aatatatgat gacagtttcc cccacaataa agattgatac agtcatgaat
    13381 actggtttag ctgtcattgg ctggtattac atccttttca gagctggtca gatgctgact
    13441 tctgtttttc tcaaacagtg gacaaaacga tgtaagcagg agcaacgcca gaaggcatta
    13501 gaagcgttct atgacgcatt tgaacttagc cgcattgaac caggcacaac agccaggata
    13561 gcgacaaaag gcgacctgat gatcgtaatg ttccgacagg agaaaactac cagtgagtga
    13621 gattgactat caggaactgc gtgaggtgac agaagcaata aaagtagtgg caacaccgcc
    13681 aaaattacgt gcgtttcgca tgaaggtcac accgcaggtt gtgctggcac tactggatga
    13741 gaacgaaagc atgtatcgcg acctgatggc tcgcaatggt gagattgaag gacttcgaaa
    13801 gctggttgct gagttcgaag ggtacaggtc gaatctgata gttaccataa aagaggttac
    13861 aaatgcttga tagttttgct cttgttgcgc tggttggcat tttagcattg tttttggtga
    13921 catccggtaa ttggagacat aagtgaacga caaagaactg attaaagaaa ttaaagagcg
    13981 cattggcagt cttgatgttc gggacaatat tgagcgccgg gcttatgaaa ttgctctggc
    14041 atcgctggaa gcagatccag tggcatggat gcatgtaaat aacggaatcg gaataccagc
    14101 aataacaagg agtaaagagg ttgcagagag ttggttatca aatggctggt atgttcagca
    14161 actatatata gccaagccag taccggtaat ccctgatgag gtgttgtcag caatcagaaa
    14221 tgttgccagg attcgcgcca atttcgctga ttttgacggt gacagaagag gtatcggtga
    14281 ttgcctggat gaggctgagc aagagttgat cattaccatt aacaaatacg cctgtcatat
    14341 tacagcagaa cagcgtagaa ggtgaagtga tgacctggcc tgaaacattc acaatggcag
    14401 gaattacaat ggcagtaaca ttgattgtgt gttcaatttg ccactgggga tagccacatg
    14461 ttcgctctga ttcaacgtgg acagatatac acggatagca ctggttatcc ggtaaaaatt
    14521 attcgcagta ctgatcactc ggtattcttc cggagaatgg atggtaatac tggtcgagta
    14581 cgcatcggtg aattcaacaa cctgtttgaa catattgacc accaagaata tcacaaaatt
    14641 ctggctgaca ctgagcagga agctcatctg aaaaaattac gctccttgaa aaggaaataa
    14701 caaatgaata aagcgtttga gctaagggta cgccagcgtt acggcaatcg ctatgacctg
    14761 acgcgagatg ttgacggctt ctactgtcgt gaagttgtga aacggatgtt tgaagtgaaa
    14821 tatggtgcca ccgctgtggc ctgaatgttg tgtgaggtga tgcatgggac ttgattgtgt
    14881 acctatatca acctactgcc gtgacgcagg agaaacgatt gatgccgtta acaaacggat
    14941 acaaagagga atatggaaag aaggggttca tgtattaaaa gtcgacggag ttaaagagcg
    15001 ttgggtcgac ttaatggaga ttacaaaatg ggcaagaaag aacaaggatc attatctctc
    15061 cctagaggag taactattcg gcagcataaa actggtagca ctctgattat cacttttaca
    15121 tataaagggg ttctgtgtcg ggagcccctc tccagaatgg aagtaaacgt gcgcggagtg
    15181 aagtacgccg agcgtctgct gggagaaata aaaaatcaga ttatcgatgg taccttcgaa
    15241 tatgcaaaat acttccccaa ctcccaaaag ctgaagctgt tcggaggagt gaaaaaaaat
    15301 aaaaatataa aatcttacct ggatgaatac ctaattatct gccagaaccg taacctgtca
    15361 ccatcgacaa ttaaaggtta tgaaaaatgc ctgtcggcgc tgtcagcact gcataaattt
    15421 catgtgtcag aactgacacc tgcagtactc aaaaactgga tcgccagcag aaaaacaaaa
    15481 ttgaaaacga tccgcaataa tctttcattt ctgcgcagcg ccatagatga agcggtgacc
    15541 gatggtttgt taacaataaa tcctgtcacg ctggtcagcg ccagccggta tcacgtattg
    15601 gacaacacac caggcaatga tgattacgag gtcgatcctt ttacgccagc agagacagca
    15661 gcaatctatg acgcctgtca ttacccggaa tggcaaaatc tgtttcgctt cgcctttaat
    15721 accgggctgc gcagctcgga attgtgcgcg ttgcgctgga cagatataga cttcatcagc
    15781 aacactgcac atgtccagac tgcaagcgta gtcggggtaa tcaaaagcac caaaacaaaa
    15841 gccggcactc gcaaagtaca actgaacaac gaagcactgg cagctcttca ggcacaaaag
    15901 ccctacacgg cgctaaaaag cgacttcata ttcagcgatc cgaaaactcg cgctccttgg
    15961 gcaaacgcag atgcgatccg caaaaaagcc tgggtaccga cccttaaaaa ggctggagta
    16021 cgctatcgca atccgtacca gacacgccac acgttcgcca ctcggcacat tagccagggt
    16081 gttaacctgt tctggcttgc tggtcaaatg gggcataaag gaccggaaat gctgttcaga
    16141 cattacggct cttaccttgc agaatacgat ggaaaaacgg caatttctgc aaccccgtaa
    16201 ctgcaaaatt tttcaaaatg ttgtacaaaa tcaggacgtt accgggacaa caatatgtac
    16261 gtaaaatgca cataatgcct tgcgtgtaaa aataaatcgt tcgtattcaa taaactatgt
    16321 gaatcccgat cacgggttca actcccgcca gctccaccaa aattctccat cggtgattac
    16381 cagagtcatc cgatgaagtc ctaagagccc gcatggcgca agccctgcgg gcttttttgt
    16441 gccttgaatt tgtcccgcga agtccgaaga gaactaatta aatccgaacc ttttaggccc
    16501 attgataggc ccaacgaaaa gctctattgt tttcgttggg cctaaacgca tggagactcc
    16561 ccatggcaag aaaaaccaag ccgttaactg atacggaaat caaagccgcc aaacctaaag
    16621 ataccgatta ccagctgtat gatggtgacg ggcttactct gctaatcaag tccagcggta
    16681 gtaagctctg gcagttccgt taccacagac cactgaccaa gcagcgaacc aaacagagct
    16741 tcggtgccta ccctgccgtc tcactttctg atgcgcgtaa actcagagct gaatctcgag
    16801 ttttgttggc gaaagacatt gatcctcagg aacatcagaa agaaaaggtg agaaattctc
    16861 aagaggccaa aaccaacacc ttcttgttag tcgccgaacg ttggtggaat gtgaagaaag
    16921 ccagcgtaac agaggactat gccgacgata tctggcgctc gcttgagaga gatgttttcc
    16981 cggcaatcgg tgatatcagt gtcactgaga ttaaggcgca tactctggtt aaagcagttc
    17041 agccggttca ggcaagaggt gcattagaga ctgttcgccg cctttgtcag cgtattaacg
    17101 aagtcatgat ttatgcgcag aacacaggct tgattgatgc agttcccagc gtaaacatag
    17161 ggaaagcgtt cgagaaaccg cagaagaaaa atatgccgag tatccgcccg gatcaacttc
    17221 cacagttaat gcaaacaatg cgtacggcaa gtatcagtat gtcaacgcgg tgcttgttca
    17281 tgtggcagct tctcaccatt acccgccctg ccgaagccgc tgaggcacga tgggatgaga
    17341 tcgatttcaa tgctaaagaa tggaaaattc cagcagctcg aatgaagatg aaccgagacc
    17401 atacggttcc actatcggat gaggctatct caattctgga aatgatgaag tctctcagtg
    17461 gtggtcgaga atttatcttt cccagtcgca tcaaaccaac ccagccaatg aacagccaaa
    17521 cagtgaatgc agcactcaaa cgtgctggct taggtggagt actcgtatca cacggtttgc
    17581 gatctatcgc cagtacagcc ctaaatgaac aaggcttccc acctgatgtt attgaggcgg
    17641 cacttgctca tgtggataag aatgaggttc gccgtgcgta taaccgcagt gactatcttg
    17701 agcaacgacg acctatgatg caatggtggg ctgctttcgt gatgacagcg gatcgcggaa
    17761 gcgtgattga aggtgggata aaaggaatac ggttagtggg gtgacaggac tgtgttaagt
    17821 tcaaggctac gccagatgtc ttcaacatga tcttgggata taccagaagt cttaatcttt
    17881 tcatccaacc actttattgc aagagcctgt agcgtgtttt cagtcgcgtc ttttagtgca
    17941 ttggcttttt cgttgttatg gacttgagga tcgataccgt tggcaagtag ggagagatac
    18001 tcatcccgca aagttcgtgc ctcagccaag ataagatgag ggttggttcc aagattcatc
    18061 tttgtgcgtt tttaggtcac ttgcactgaa tacatgaaat accatttttt cttcccactt
    18121 tttgtgtctg gcgctatacg tagcagtaat ctatctccgt caaagacggt tgatttcctt
    18181 atcgccgggt ttggtattat taatttcagt atcagtaagt ttttttgcga gctttgccat
    18241 ttgggaccct caagttttgg cccctttccg ttgggtccca aaaagggtgc cataagttgt
    18301 ggttctcagc aattctcact gaactatagt gaacgtaaaa aatcccgcag gccttgcgcc
    18361 atgcgggctt agtgaactta actgaacgtt agtgtatcaa tatttggtgg agctgactga
    18421 aagcgatacc aaatactaac tgcctgattc tttaagcaat gtaacggaaa tcaatgtcac
    18481 aacagtatgg cgcaagtcat ttgtatcaat caagattaag agagggagtc atgattggca
    18541 agagaccttc taagcttttg gacacgagta aactctagcg tctctaaaaa caaatctgaa
    18601 agttcttgtc gggaaatcac aacgtcatca ctaacttgcc atgctatccc aacctggtag
    18661 tcaaatcctg gatatgcaat cttatagtca gtcttattgt gcgctttagc ggcttcaaat
    18721 ttagctaagg gtatccttaa gcgtgcaaaa cgtttctttg cattctgaaa acgctctaac
    18781 acttccgcag ggtcatcact tttgttttcc ttaatgctct gcgtaagagt gcgaattact
    18841 tcataagagc ccaaaaccca taaatatgag tgagtaagat gcccttgtat cagcttgtag
    18901 tcttcgtttg catttccgcc ctgcttatac ttttctacta aagaaacatc catctcacaa
    18961 agagttgcat ccattttccc caaactttgc acaaggttcg gtataaaagg atcaaatcgt
    19021 gccaaaccaa tagaaacttt cagccagcgt tcaaaacgct cctcatacat gacactatcc
    19081 atttgttcta gccctttaaa atcagagaat taatcttggt agctaacctt acccacgagg
    19141 gttctgttga taatactcat tcagtccatc agtgtatcct tggtagtagg ctacacgagg
    19201 gcttctgtgg cgaacttctg ccgcttgact ttttaatatg gagtcaatag ccgcagctgc
    19261 atttggctct tggttagtta cccccgcttg aaaacccaat tcgtatgcac atccggcgca
    19321 tttatgacgg aatggatagc tttgatcctc tgggagagct gcaaacatgg tgtcgtagcg
    19381 gtgcggtgct tggcaggttg tcatgagtac ctcttgaata ttatgaaagt actctttata
    19441 tgagtacggt ttttattcat tcaagaagaa ctgggcctgc tttcatctct tttcttacag
    19501 aatgtgagct acttcgattt ttgggatact gcgtcactaa gcatgaactc aaatccctga
    19561 ctgggaagct gaatttacta gataagctgt cgctgccggt atccgtcctt atcccattaa
    19621 taatcgatgt ttaggattca gataacatat atcggctata gaagaagcgg gaaaggtgtg
    19681 aatacttcga gagatgtcac tgttgaaaga tgacaaaaat accaagcaga tttggcacag
    19741 ctaccgctca tatactgaaa aaaatgacgt ggatgtttct ttatgcagtt aaaaaaggcg
    19801 ctaggaatca aatatcccat tcattctcaa tccaatattt taacttattt aaaaagaatt
    19861 accccctctc agttactgca ttgataatta accaattcca attaacatct acctttttac
    19921 aacctctgaa aatatctctt tcaactcaat atcatttcta gaccagtcgc tgattgttct
    19981 taataatttt tcccatgagg tccaatcatg gtttttacta gtatcttttt caataaattt
    20041 agtgatctcc ttctccaaag agttgatagc ggctacttta ctcttccaac cattttcagg
    20101 catatttttc ttcaataatc gaataacttc atccttgact cgtctataat tctgcgattt
    20161 atttttgcca ccaaggcttg cagcctctcg ttttatctta tctgactgct ctttagcagc
    20221 taccatttcc agagcccagt tggctccagt ccacatatac atcagtttta gtgctatcaa
    20281 agcgtgttta cgcccttcag ctttatggcc caacttgtac tgtgacattc cccacttatg
    20341 ggatacaaaa gctgcttcca gaaatttact gggctccccc tgccatctgg cagcagactc
    20401 catactacta acatattctt cgatagtgag ttcccgataa gtatatttgt cagcctgaat
    20461 cttgccattt ctgtaatcag taatcacggt aagggcatct tcctcgagct tacggatctt
    20521 cctctcggca gaattctgtt taaagtgact aacatctttc actttcttca cacatctctc
    20581 aaattcatct agaagctctt cgaaggcaac atcaaaatct ttatttttca ttgaattacc
    20641 aaaaagtgaa tgtgaagtac caaaatataa aaattacttt atgaaggcag attttttgat
    20701 actccctccc gcattactat aagcaccaat ccagaccaat caagagtaat gaactatgcc
    20761 tacacaaact ataaagattt taagattacc tgaagtagtc aagaaaatcg ggatcaaaag
    20821 atctaccata tacgactggc ttaacccaaa atcaccacgc tacgacaaaa cgttcccaaa
    20881 acaaaaacgc ctgggaaggc aatccgttgg ctggctggag tcggacctgg acgaatggtt
    20941 aagcccacgt tagataccac ttacattaac gatccggggg gcaatgcccc cagtttgctc
    21001 cacaaaatca cattcctccc cggaattcga atgcaaaaaa aaacagacct gtattaccgc
    21061 aatgaataca ttgcagcgaa gttgctgtat cgaatttttt tcattccgga ggctacatgt
    21121 ctgattcctt tgaacggggc attaatgtcc tgaagcaacc tttatccatt cttcctgaac
    21181 atatctccga tttggttctg gagcatatac gccagagtat tcattacgaa cccgtgattg
    21241 gcatcatggg caaaacaggt gccggaaaat cctcgctttg caatgccctt tttgaacatc
    21301 ctctcagccc agtcagccat gcagaaggct gcacacgtaa acctttgcat tttaccctga
    21361 acgcaggagg acgcagcctg acgctggtcg atctgccggg cgcaggtgaa tcacttaagc
    21421 atgatgagat atatcgccag atgtaccagg aacagttacc gaagctggat ctcattctct
    21481 gggtgatgaa agccgatgac cgagcctgta ccacagacga agagttccac cgttttctac
    21541 tggattgtgg cgtatcgaca gacagcattg tttttgttat caaccaggct gacaaagccg
    21601 agccatcact ggaatgggat cgtgaaaaaa gatctccttc agtggagcaa ctcatgacgc
    21661 tcacagcgag aagtgctgcc gtttcccgcc agttctttac cccttacccg gtgcatgcag
    21721 tttcagcaaa aaccggttac aacatgtctc gtatagttga aacaatgatt tttgccctgc
    21781 caccagaggc tactagtggc gtctttcgcc agatcaggaa agaacatcgc accaaagaat
    21841 cggagacgaa ggcccgaaag gatttcgcgg atttgttggg agaattattg agcaggattc
    21901 tggaggccat acctcttccc aacgtgatac gcaacgggct gggagtgctc agagaagggc
    21961 tggagaatgc agctaccgca atatggcact ggtttttcta atacatctgc ccactgagtt
    22021 aagccacctg aaaattatga ttttcagcga caggatatgt cgcgacctgc ttcagttctg
    22081 tctctgaaaa aaatagttat tgttaatcag gatattactt agttatttgt ctggacgttt
    22141 tttctctatt cctccc
//
LOCUS       DAZFFG010000082        21539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFFG010000082 DAZFFG010000000
VERSION     DAZFFG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.082"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..215)
                     /locus_tag="ACHJ7J_003386"
     CDS             complement(<1..215)
                     /locus_tag="ACHJ7J_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
                     /protein_id="HFO5001573.1"
                     /translation="MFPLNDLSLKTQPVQLNKVTSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNGKFTPISEDSKLFNMLSEF"
     gene            complement(566..880)
                     /gene="fliE"
                     /locus_tag="ACHJ7J_003387"
     CDS             complement(566..880)
                     /gene="fliE"
                     /locus_tag="ACHJ7J_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5001574.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1095..2753
                     /gene="fliF"
                     /locus_tag="ACHJ7J_003388"
     CDS             1095..2753
                     /gene="fliF"
                     /locus_tag="ACHJ7J_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5001575.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2746..3741
                     /gene="fliG"
                     /locus_tag="ACHJ7J_003389"
     CDS             2746..3741
                     /gene="fliG"
                     /locus_tag="ACHJ7J_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5001576.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3734..4420
                     /gene="fliH"
                     /locus_tag="ACHJ7J_003390"
     CDS             3734..4420
                     /gene="fliH"
                     /locus_tag="ACHJ7J_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5001577.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEETEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4420..5793
                     /gene="fliI"
                     /locus_tag="ACHJ7J_003391"
     CDS             4420..5793
                     /gene="fliI"
                     /locus_tag="ACHJ7J_003391"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5001578.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISPE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5812..6255
                     /gene="fliJ"
                     /locus_tag="ACHJ7J_003392"
     CDS             5812..6255
                     /gene="fliJ"
                     /locus_tag="ACHJ7J_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5001579.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNRWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6252..7379
                     /gene="fliK"
                     /locus_tag="ACHJ7J_003393"
     CDS             6252..7379
                     /gene="fliK"
                     /locus_tag="ACHJ7J_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5001580.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7484..7948
                     /gene="fliL"
                     /locus_tag="ACHJ7J_003394"
     CDS             7484..7948
                     /gene="fliL"
                     /locus_tag="ACHJ7J_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO5001581.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7953..8957
                     /gene="fliM"
                     /locus_tag="ACHJ7J_003395"
     CDS             7953..8957
                     /gene="fliM"
                     /locus_tag="ACHJ7J_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO5001582.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8954..9367
                     /gene="fliN"
                     /locus_tag="ACHJ7J_003396"
     CDS             8954..9367
                     /gene="fliN"
                     /locus_tag="ACHJ7J_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO5001583.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9370..9735
                     /gene="fliO"
                     /locus_tag="ACHJ7J_003397"
     CDS             9370..9735
                     /gene="fliO"
                     /locus_tag="ACHJ7J_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO5001584.1"
                     /translation="MNNHATVQSSAPISAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVDDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9735..10472
                     /gene="fliP"
                     /locus_tag="ACHJ7J_003398"
     CDS             9735..10472
                     /gene="fliP"
                     /locus_tag="ACHJ7J_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5001585.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10482..10751
                     /gene="fliQ"
                     /locus_tag="ACHJ7J_003399"
     CDS             10482..10751
                     /gene="fliQ"
                     /locus_tag="ACHJ7J_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO5001586.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10759..11544
                     /gene="fliR"
                     /locus_tag="ACHJ7J_003400"
     CDS             10759..11544
                     /gene="fliR"
                     /locus_tag="ACHJ7J_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5001587.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            11834..12457
                     /gene="rcsA"
                     /locus_tag="ACHJ7J_003401"
     CDS             11834..12457
                     /gene="rcsA"
                     /locus_tag="ACHJ7J_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO5001588.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12501..12689)
                     /gene="dsrB"
                     /locus_tag="ACHJ7J_003402"
     CDS             complement(12501..12689)
                     /gene="dsrB"
                     /locus_tag="ACHJ7J_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO5001589.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12852..13079
                     /gene="yodD"
                     /locus_tag="ACHJ7J_003403"
     CDS             12852..13079
                     /gene="yodD"
                     /locus_tag="ACHJ7J_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO5001590.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13377..14192
                     /gene="yedP"
                     /locus_tag="ACHJ7J_003404"
     CDS             13377..14192
                     /gene="yedP"
                     /locus_tag="ACHJ7J_003404"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO5001591.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTTRLSELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(14189..15883)
                     /gene="dgcQ"
                     /locus_tag="ACHJ7J_003405"
     CDS             complement(14189..15883)
                     /gene="dgcQ"
                     /locus_tag="ACHJ7J_003405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO5001592.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELAMLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGNFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLIEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(16126..16308)
                     /gene="yodC"
                     /locus_tag="ACHJ7J_003406"
     CDS             complement(16126..16308)
                     /gene="yodC"
                     /locus_tag="ACHJ7J_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodC family protein"
                     /protein_id="HFO5001593.1"
                     /translation="MSFMVSEEVTVKVGDPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPVEEV"
     gene            complement(16387..17304)
                     /gene="yedI"
                     /locus_tag="ACHJ7J_003407"
     CDS             complement(16387..17304)
                     /gene="yedI"
                     /locus_tag="ACHJ7J_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO5001594.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            17477..18397
                     /gene="yedA"
                     /locus_tag="ACHJ7J_003408"
     CDS             17477..18397
                     /gene="yedA"
                     /locus_tag="ACHJ7J_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO5001595.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18386..18856)
                     /gene="vsr"
                     /locus_tag="ACHJ7J_003409"
     CDS             complement(18386..18856)
                     /gene="vsr"
                     /locus_tag="ACHJ7J_003409"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO5001596.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18837..20255)
                     /gene="dcm"
                     /locus_tag="ACHJ7J_003410"
     CDS             complement(18837..20255)
                     /gene="dcm"
                     /locus_tag="ACHJ7J_003410"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO5001597.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISGCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(20322..21017)
                     /gene="yedJ"
                     /locus_tag="ACHJ7J_003411"
     CDS             complement(20322..21017)
                     /gene="yedJ"
                     /locus_tag="ACHJ7J_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO5001598.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     YRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(21057..21338)
                     /gene="drpB"
                     /locus_tag="ACHJ7J_003412"
     CDS             complement(21057..21338)
                     /gene="drpB"
                     /locus_tag="ACHJ7J_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO5001599.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5229 a         5690 c         5519 g         5101 t
ORIGIN      
        1 ttaaattctg acagcatatt aaataatttg ctgtcttcac taattggtgt aaacttacca
       61 tttccaagac aactgactaa ctcacttgat aattcattta tgattgcatc atcagatact
      121 aactcatgtt gttttatcgt agattcagta ttagatgtga ctttatttaa ttgaaccggc
      181 tgagttttta acgaaagatc atttaatgga aacatttaca ctcctgttta ataaattata
      241 ttgcccaggg agcattctaa aacacaataa aattaccact atcaaaaatc gctcattttt
      301 ataacgcagt atattaataa aaataagaac gaagattaaa ttatccagag attactgttg
      361 cgtctgtggt cgtaacaaca tccgacgtgg tgatgtgtgt caaatgagac attctgatga
      421 tcataaggtt tatcgcgtat acggatcgtg gatggcagaa aactttcaga atcaggtttc
      481 cattttaaac tagaaattga gtgagtttgc cccatccatg ccccacgcgg tgagatcgga
      541 tgtaattaac aacaggttac aaaacctaca cctgcatgct catcacttcc tgatacgccg
      601 ccaccagctt attacgcacc tgaatcccca tttgcataga aactgaggct ttttgcatat
      661 cggtcatcac atcgtttaac gccacgccgg gttcaccgag agtgaatttt tctgcctgcg
      721 tgcgggcggc tgtttgtgta tcgcttatgc gatcgagcgc ggcgtgcagc tgcccggcaa
      781 aactaatggt cggttgcggc agtgattcct gcgcacgcgc actcatcgcc gtagcctgta
      841 actggctgat aaccccttca atcccctgta tcgctgacat tttcatctcc tggataattt
      901 ctggtagcaa agcctaccag taagtcaata agacaaaggc gctaaatagc aacaaaaaaa
      961 cgggtttatt ggcggataga aaaaaacgaa agcacaaata atgggagcgt caatttttcg
     1021 agtttgctga cccgggagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
     1081 ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
     1141 gcctgcgtgc gaatccgaaa attccattga ttgttgccgg ttccgcggca gtggcggtca
     1201 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
     1261 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
     1321 tcagcgaagc cagcggcgct attgaagttc cggcagataa agttcacgag ctgcgcctgc
     1381 gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaacta ctcgatcagg
     1441 aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggtg
     1501 agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa
     1561 tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa
     1621 atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgccattgtg catctggttt
     1681 ccagcgccgt tgctggtctg ccgccgggaa acgtcacgct ggtggatcag ggcggacatc
     1741 tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcaattg aaatatgcca
     1801 gcgatgtcga aggccgtatt cagcggcgta ttgaagcaat cctgtcgcct attgttggta
     1861 acggtaatat tcacgcccag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg
     1921 aagaacagta tcgccctaat ggtgatgaat ctcaggcggc gcttcgttca cgccagctta
     1981 atgagagcga gcaaagcggc tccggttatc cgggcggcgt accgggggcg ttgtcgaatc
     2041 aaccggcacc tgcgaataac gcgccaatca gcacgcctcc ggcaaatcaa aataaccgcc
     2101 agcagcaggc gagcaccacc agcaatagcg ggccgcgtag cacacagcgg aatgaaacca
     2161 gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac
     2221 gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccgttgcctc
     2281 tcagcaacga acagatgaag caaattgaag atctgacccg cgaggcgatg ggcttttctg
     2341 aaaaacgcgg tgactcgctc aatgtcgtta actcgccgtt caatagtagt gaggaaagcg
     2401 gcggagaact gccgttctgg caacagcaag cgtttatcga tcagttactg gctgccggtc
     2461 gctggttgct ggtactgctg gtggcgtggc tgctgtggcg taaagcggta cgcccgcagt
     2521 taacacgtcg cgctgaggcg atgaaagctg tacagcaaca ggcgcaggcc cgcgaggaag
     2581 tggaagatgc ggtggaagtc cgcctgagca aagacgaaca actccaacaa cggcgcgcta
     2641 accaacgtct gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
     2701 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
     2761 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
     2821 ttcaagcacc tctcccagcg cgaggtgcaa accctgagcg ctgcaatggc gaacgtcacg
     2881 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
     2941 tttgccgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
     3001 ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaactcgcga taccgccagc
     3061 ggtattgaaa cgctcaactt tatggagcca cagagcgccg ccgatctgat tcgcgatgag
     3121 catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt
     3181 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
     3241 ggcgtgcagc cagccgcgct ggcagagctg accgaagtac tgaatggctt gctcgacggt
     3301 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
     3361 atgaaaactc agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgaactg
     3421 gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt cgacgatcgc
     3481 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgttgatcgc gctgaaaggg
     3541 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
     3601 ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtgga aaacgaacag
     3661 aaagcgattc tgctgattgt gcgccgcctt gccgaaactg gcgagatggt aattggcagc
     3721 ggcgaggata cctatgtctg ataatctgcc ctggaaaacc tggacgccgg acgatctcgc
     3781 gccaccacag gcagagtttg tgccaatagt tgagccggaa gaaaccatca ttgaagagac
     3841 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggcccacg agcaaggtta
     3901 tcaggcgggg attgccgaag gtcgccagca aggtcataag cagggctatc aggaaggact
     3961 ggctcagggg ctggagcaag gtctggcaga ggcgaagtct caacaagcgc caattcatgc
     4021 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcacttg atagtgtgat
     4081 agcgtcgcgc ctgatgcaga tggcgctaga ggcggcacgt caggtgatcg gtcagacgcc
     4141 aacagtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
     4201 attcagcggt aaaccacagc tgcgcgtgca cccggatgat ctgcaacgtg tggatgatat
     4261 gctcggcgct accttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccatcc
     4321 tggcggctgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
     4381 gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg cctgactcgc
     4441 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
     4501 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
     4561 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
     4621 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt
     4681 gtcctgcccg gcgcgcgtgt ttatgccaaa aacatttcgc ctgaagggct acaaagcggc
     4741 aaacagttgc cgctcggtcc ggcgttatta ggtcgcgttc tggacggcag cggtaaaccg
     4801 ctcgatgggc tgccctcccc cgatacgact gaaaccggtg cgctgattac cccgccattt
     4861 aacccattgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
     4921 gccctgctta ccgttgggcg tgggcagcgt atgggactgt ttgccgggtc cggcgttggt
     4981 aaaagtgtgc tgctggggat gatggcccgt tacacccgcg ccgatgtcat tgtcgtgggt
     5041 ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaacatcct cggcgccgaa
     5101 gggcgtgcac gctcagtggt gattgccgct ccggcggatg tgtctccgct cctgcgaatg
     5161 cagggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg
     5221 ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg
     5281 attggcgaac cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca
     5341 ctggtcgagc gtgccggaaa tggcattagc ggcggcggct cgattaccgc gttttatacc
     5401 gtgctcaccg agggcgatga ccagcaggac cccattgccg actccgcgcg ggcgatcctc
     5461 gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat
     5521 attgaagcgt cgatcagccg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga
     5581 gtgcgcacct ttaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc
     5641 ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag
     5701 ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag
     5761 gggctggagc gtattttccc gacagtgtca taacccagga gataacggta gatggcagaa
     5821 catggtgcgc tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgcacgc
     5881 ctgctgggtg aaatgcgtcg cgggtgtcag caggcggaag aacagctcaa aatgctgatc
     5941 gattatcaga atgaatatcg caataacctc aacagcgata tgagtgccgg aatgaccagc
     6001 aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat
     6061 cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaaccg ctggcgagaa
     6121 aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg
     6181 cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg
     6241 aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgat accaccacat
     6301 tgcctggcgg caaagccagc gatgctgcac aagattttct cgcgttgttg agcgaagcat
     6361 tagcaggcga gacaactacc gacaaagcgg ccccccagtt gctggtggca acagataagc
     6421 ccacgacaaa aggcgagccg ctgatcagcg atattgtttc cgacgcgcaa caagcggatt
     6481 tactgatccc tgtggatgaa acaccgcctg tcatcaacga cgaacaatcc acatcaacac
     6541 cgttaaccac cgctcagacg atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag
     6601 acgaaaaagc ggatgatctg aatgaagacg tcaccgcaag cctgagcgcc ctttttgcga
     6661 tgttgccggg ttttgacaat acgcccaaag tgactgatgt gccgtcaacc gtgttaccgg
     6721 cagagaaacc aacgctcttc acaaaactga cttctgcgca actcacaaca gcacagcctg
     6781 atgatgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa gcccaaagta
     6841 aagcggaagt catcagcaca ccttcaccgg tgaccgctgc cgccagcccg ctaatcactc
     6901 cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt
     6961 ctcacgaatg gcaacaatca ttaagccagc atatttcgct gttcacccgc caggggcaac
     7021 aaagtgcaga gttgcgtctg cacccgcagg atttaggtga agtgcaaatc tccctcaaag
     7081 tggatgataa ccaggcgcaa atccagatgg tttcaccgca tcagcatgta cgcgccgccc
     7141 tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc
     7201 aaagcaacat cagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc
     7261 aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg
     7321 ttcccgtctc tttacaaggg cgtgtaacag gcaacagcgg cgttgatatt ttcgcctaac
     7381 gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga
     7441 taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat
     7501 aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc
     7561 ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc
     7621 gcagcaacgc gtcgtgccct caccggtctt ctatgcgctg gataccttta cggtcaatct
     7681 gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgcctgaaag atgaagctac
     7741 ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc
     7801 gcgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat
     7861 taaaaccacg ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt
     7921 gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg
     7981 aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta
     8041 gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac
     8101 gtttgcaggc gctggaaatc attaatgagc gctttgctcg ccattttcgt atggggctgt
     8161 tcaacctgct gcgccgtagc ccggatatta ccgtcggggc catccgcatt cagccgtacc
     8221 atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa ccttatccat ctgaaaccgc
     8281 tgcgcggcac tgggctggtg gtgttctcac cgagtctggt gtttatcgcc gtggataacc
     8341 tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccataccg
     8401 aacagcgcgt catcaaccgc atgttgaaac tggcgcttga aggctatagc gacgcatgga
     8461 aggcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg aaatttacca
     8521 atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca
     8581 acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat
     8641 tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc
     8701 tggtgcgcca ggtgcagcat tcacagctgg agctggtcgc caactttgcc gatatctcgc
     8761 tacgcctgtc gcagatttta aaactgaaac ccggtgacgt cctgccgata gaaaaacccg
     8821 accgcatcat cgcccatgtt gacggcgtcc cggtgctgac cagtcagtac ggcactctca
     8881 acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg
     8941 aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga
     9001 cgatctgtgg gctgaagcgt tgagcgaaca aaaatcaacc agcagcaaaa gcgctgccga
     9061 cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct
     9121 gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa
     9181 agagctgttg cgtctgacgc aagggtccgt cgtggcgctg gacggtctgg cgggcgaacc
     9241 actggatatt ctgatcaacg gttatttaat cgcccagggc gaagttgtcg tagtcgctga
     9301 taaatatggc gtgcggatca ccgatattat taccccgtct gagcgaatgc gtcgcctgag
     9361 ccgttagtga tgaataacca cgctactgtg caatcttccg cgccgatttc tgctgcgcca
     9421 ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg
     9481 ctggtaaaac ggttgggatt tgcccctaag cgcacaggcg ttaacggtct gaaaattagc
     9541 gccagtgctt cgctgggcgc gcgtgaacgg gttgtggtgg tcgatgtgga tgatgcacgg
     9601 ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttctgca
     9661 ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt
     9721 agcgggagat cctgatgcgt cgtttattgt ctgtcgcacc tgtccttctc tggctgatta
     9781 cgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctgcct ggcggtggac
     9841 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg tttattccgg
     9901 caattttact gatgatgacc agtttcaccc gcatcatcat tgtttttggt ttattgcgta
     9961 acgcgctggg aacaccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    10021 tgaccttttt tattatgtca ccggtgatcg acaaaattta tgtcgatgcg taccagccat
    10081 tcagcgaaga gaaaatatca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    10141 agtttatgct gcgtcagacc cgtgaggcag atttagggtt gtttgccaga ctggcgaata
    10201 ccggcccgtt gcagggacct gaagccgtgc cgatgcgcat tttgctcccg gcctacgtga
    10261 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    10321 tcgacctggt gatagccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    10381 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgcttg
    10441 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    10501 tgatgatggg gactgaagcg atgaaagtcg cgctggcact ggctgccccg ctattgttgg
    10561 tagcgttggt cacgggcctt atcatcagta ttttgcaggc cgccacgcag attaacgaaa
    10621 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccattatt attgccggac
    10681 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    10741 tcatcgggta gccgtactat gttgcaggtg acaagcgaac aatggctatc ctggttaagc
    10801 ctgtacttct ggccgttact gcgcgtgctg gcgctcattt ctaccgcacc tattctgagc
    10861 gaacgcagcg tgccgaaacg ggtaaaactg gggctggcaa tgatgatcac gttcgccatt
    10921 gccccatcat tacctgccaa cgatgttcct gttttttcgt tctttgctct gtggctggcc
    10981 gtgcagcaga tcctgatcgg cattgcgctt ggttttacca tgcaatttgc ctttgccgct
    11041 gtgcgaaccg ctggcgaaat tatcggtctg caaatggggc tgtcatttgc gacgtttgtc
    11101 gatccggcca gccatctcaa tatgcccgtt ttagcgcgta tcatggatat gctggcgtta
    11161 ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt
    11221 cacaccctgc cgattggtgg cgaaccgttg aacagcaatg cgtttctggc actcaccaaa
    11281 gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat tactctgctg
    11341 ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt
    11401 gttattggat ttccattaac tctgactgtc ggtatctctt taatggcggc attaatgccg
    11461 ttaattgcgc ctttttgcga acatttattc agtgagatgt ttaatttgct ggctgatatt
    11521 attagtgaat tgccattaat ataattccgt aacgtttatc atgttatcct aaggattatc
    11581 cgaaaaataa tacctacgaa catcttccag gatactcctg cggcgaaata tttgttttaa
    11641 gctcactcac atatcgcaac atttacttta ctttaagaca attccaggca aattatacaa
    11701 cactttacgg gatagtaagt ccgcctgaaa aatcgcgaga gtggcgcatt aggtgaccca
    11761 tgttgttccg tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga
    11821 gtgagggtat gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt
    11881 aaccgggtat ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt
    11941 tgatgacctt gccatagctt gtgattcaca gcgcccttca gtggtgttta ttaatgagga
    12001 ctgtttcatc cacgatgctt ctaacagtca gcgtatcaag ctcatcatta atcaacatcc
    12061 caatacgtta tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt
    12121 ggtcagaaaa aatttattga tcagttctaa atcgattaaa ccggaatctc tcgacgatat
    12181 ccttggcgat attctgaaaa aagagacaac gataacctcg tttttaaata tgccgacgtt
    12241 atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat
    12301 tcaaatctcc gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat
    12361 taaacgtaag atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga
    12421 taatgtgact aatggtattt ttgtcaacat gcgctaacac attctgactg gtggtttccc
    12481 accagtcagg ctgaataaga ttactctgct ttctccacaa agataccgtc ctgatgccct
    12541 gcttcattaa agaaccagat gccgagcggg tagtcttcca gcgaaaccag gtacattgtg
    12601 ccttcactaa actcctcaac tgccagtacc acgccaggac gacgcggacc gccatccgtt
    12661 ttgactgtta cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta
    12721 gaacaatttc gtttttctgg cagcgccagg cgcgcgcgag tgctgatttt ctcgacggtc
    12781 tatacttaag agatgccagg cggacttaac gactggcggc aacaacagag taacggttgc
    12841 gaggaaagat gatgaaaacc gcaaaagagt acagcgatac cgcaaaacgt gaggtcagcg
    12901 tcgatgtcga tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca
    12961 gccagaacga ttctgaacac gttagcgtcg atggacgtga atatcataca tggcgtgaat
    13021 tggcggatgc cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa
    13081 tgcacaataa aaaaatcccg accctgaggg ggtcgggatg aaacttgctt aagcaagaag
    13141 cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc atcaccttat ccgcaatttt
    13201 tttcgctgac aagaaaatat tcgccatatg aatgattaac gctacttatg aatagaaatg
    13261 tgacgcacgt caccccttgg tggctctggc gtcagcggga tcgctcccga aaaaatcatc
    13321 tttgataatt tgtgctgttt tgagaatcat cctggtaagg ggagtactgc gcgactatgt
    13381 tttcaattca acaaccacta ctggttttta gcgatcttga tggcaccctg ctggacagtc
    13441 atagttatga ctggcaaccg gcagccccct ggctcagccg tttacgcgaa gcaaatgttc
    13501 ccgtcattct ctgtagcagt aaaacatcag cggaaatgct gtacttgcaa aaaatgttgg
    13561 ggctacaagg tttaccgctg attgcagaga atggcgcagt gatccagctt gctgaacaat
    13621 ggcaggagat agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatcagcc
    13681 aggttttaaa tacgctacgc gagaaagaac attttaaatt cacgactttt gatgatgtcg
    13741 acgatgcaac catcgccgaa tggacgggat taagccgtag ccaggcggcg ctgacgcagc
    13801 tacatgaggc gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta
    13861 ccactcgtct gagcgaactg ggcttacagt ttatgcaggg tgcgcgcttc tggcacgtac
    13921 tggatgcctc tgccggaaaa gatcaggctg ccaactggat tatcgcgacc tatcaacaat
    13981 tgtcaggcaa acgcccaacc acacttggcc tgggcgatgg gccaaacgat gcgcccttac
    14041 tggaggtaat ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg gtgcatctgc
    14101 atgatgagga tccggcccgc gtctggcgaa cgcagcgtga aggaccggaa ggatggcgtg
    14161 aagggctgga ccattttttc tccgcccgtt aagcgttatc gctcgcgaat acccgattac
    14221 gcccggcctg tttagcgaga taaagccgac ggtcggccag tgactggagt tgttcaaaat
    14281 cataatcacc ggtttcctcg ctgctgctta cccccagcga ggcactgatg cgtatcgtcg
    14341 tactcttggc gatcaacatc tctttttcaa ttaacttcag gcgaatacgt tctgcgacct
    14401 ccgcagcctc cgtcagactc gcgcctggca gaatcacaca aaactcctca ccaccgaccc
    14461 gcccggcaac gtcctgcgca cgcaaggaac tgctaattaa tccggcagca tgagaaagaa
    14521 cacggtcgcc cgcctgatga ccaaagcggt cattaatcgc tttaaaatgg tcaagatcga
    14581 cctgaatgac agaaaaagga tgttggtgcg tctgacacaa tttcgcgagc ggacgggctt
    14641 tttcgaacag tgcgccacgg ttatataaac gcgttaaggt gtcgtgccac gcctgccact
    14701 gcaacgagct ttgcagaaca tacatgttgc tgaccatccg gcgaatcaca taccaggaga
    14761 tgagtaacat ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg atactgccga
    14821 aattgccgcg cacgccttcg cttaacgtat ggacgcgcac cagcacaccg tcgaaatggt
    14881 ccagacgttc ccagctaaca tagcgactgt ccatacgaat gccgccccgc gtgtcatgtt
    14941 ccattgcctg cgccagcatg gccagttcac gaggatcaaa aatattccct gttggatggt
    15001 caggattgga agaggtcaaa aatctcagct tactgtcata gagctgatac tcaccatcga
    15061 ggtttttatc gatggcgttt cttaaaaatt gctgcatagt acgcacggga atactcatcc
    15121 ccagcacgcc ataccagtag ttattactgt ctaccggaac actgacggta acctgcggtt
    15181 cagtattgct ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt
    15241 ctcgttgtga atggccgata aaccagggtt gggtgacata gccgtaataa cgcgtcggga
    15301 cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa tccggcacgc gagacataca
    15361 tcgcctgttc taccatcgac gaggagttgt gcgctagtcg cagcaagtaa ccaacttcca
    15421 gtgcagcggt aatttcattg tcgaggcttt cattttcgcg agacaggaga ttcccctcgc
    15481 tgactaatgc atccgacacg ccattgactg gcagggtgcg ccgtcggttg agttcgattt
    15541 tccaggcgtg ctcgtcgcga tgctgttcga actcggttac agcatcacgc agagaggtga
    15601 aatccagtgg cgctacgaga gcttcgcgca tgccattacg caaaaagatc agtttgtcga
    15661 cgttatactg caaatgctta tcgagcgcgt tggctacgtt ttccagatga ttacgctggc
    15721 tggagatata ggcatcttcc agcactacca cttcacgcca ggtgagcaag gtggaaaaaa
    15781 gcaataccac gataaagcag agattaacga catgaccagg ccccaggcgg cgtgcgagtt
    15841 ttttcaacca gctctggttt tccatttttg tctcgtgctg caccctgacg actcccaacc
    15901 tgctttttta tgattctggc ataacctggc gatagcgtac cgcaaaacag cgataatttt
    15961 ataatggtgg catgataaga cgcgacagac atcgatagtt ttgttttata acgtaggcat
    16021 gataagacgc gatagcgtcg catcaggcaa ttcagtgcag acactggcac acccgaccgg
    16081 atgcgacgtg agcgccacat ccggcctggt gttaatgatt ccctttcaaa cttcttccac
    16141 aggacgactc ccctcccccg gaacaagctc ggtttcatga aaagcttccc gcttgacccc
    16201 gtaaccgtca taccaacgac actcaaccat accgctggag tatccagtga caatcatccg
    16261 cgggtcgccc actttaaccg taacttcctc actaaccata aagctcatac tcgcctcctt
    16321 ttttcgagtg aaacttgttc accttagttg aagatggcga attttgcata atagcctgtg
    16381 cgagtattaa tgtgcctgac cgcgcatttt cgctacggct ttcactccca gcaccacgat
    16441 gccgccgatg ataaatccaa gaatcagatt taaaacagtc ggtaatatca tcgccaccac
    16501 tgcactttgc tgcccggcga aatgttcaat cgcgtgatgc agcggcgcaa taccatgtac
    16561 aacaatcccg ccgccgacga ggaacatcgc cagcgtgccg acaatcgata acgctttcat
    16621 cagccagggc gcgataatca ataacccttt acctaatgcc tgcatcagcg cgctggattt
    16681 ttccgccagc caatacccca ggtcatcaat cttaacgata acccctacca gaccgtagac
    16741 gcccacggtc actaccagcg cgatgcctga aagcaccagc acctgattaa gcaacggcgc
    16801 ttcggccaca atccccagcg tgatggcgac gatttcggca gacaatataa aatcggtacg
    16861 aatcgcccct tttattttgt ccttttcaaa cttcagcgga tcctgcgccg ccagcttctc
    16921 cagacgctgc tgactctgcg ccggatcttc tttatgttta cgcgcctcca gcatatgcag
    16981 cactttctct accccttcaa agcagagaaa cgcgccacca atcatcaaca gaggcgtaat
    17041 cgcccacggg ataaacgcac tgatgatcag cgccagtggc accagaatca ctttattgat
    17101 cagcgatcct ttcgccacgc cccagaccac gggaagttcc cggttggccc tcacgcctga
    17161 aacttgttgc gcattgagcg ataagtcatc ccctaataca ccggcggttt tcttcgccgc
    17221 cagtttgccc atcacggaga tatcgtccag cagtgtggcg atatcatcga gcaacgtcag
    17281 taaactactc ccggccaaaa gcattccctc tcatttttat tgttgaaggg tgtaagtatg
    17341 aagcaaaaac ggcagctctg acacttgctt cacaggtcaa cattttttta acaatcaagt
    17401 gcaattatat ctctcgccag cagaatgatt ttcacgttta ctatgaacca cctttttatt
    17461 tccaccgtga gggattatgc gtttccgcca gttgttaccg ctttttggcg cgctgtttgc
    17521 gttgtatatc atttggggct caacctattt tgtcattcgg attggcgtgg aaagctggcc
    17581 tccgttaatg atggcgggcg ttcgattcct ggcagccggt attttattgc tggcattttt
    17641 gctactgcgc ggacacaaac tccccccgct acgtccgctg ctcaatgccg cgctgattgg
    17701 cctgttattg ctggctgtcg gtaatggcat ggtgacggtt gccgaacatc aaaatgttcc
    17761 ttccggcatc gccgccgtag tggttgcaac cgtgcccctc tttaccctgt gcttcagccg
    17821 cctgtttggc attaaaacgc gcaaactgga atgggtgggt atcgccattg ggcttgccgg
    17881 aatcatcatg ctcaatagcg gtggaaattt aagcggcaat ccgtggggcg cgattctgat
    17941 tttaatcggc tcgattagct gggcgtttgg ctcggtttat ggctcgcgca ttaccttacc
    18001 tgtagggatg atggcgggtg cgattgagat gctggcggca ggcgtggtgt taatgatcgc
    18061 gtcgatgatt gcaggtgaaa aactgacggc gctcccttcc ctttcaggct tccttgcggt
    18121 cggctatctg gcgctgtttg gttcgattat cgccatcaac gcttatatgt atttaatccg
    18181 caacgtcagt ccggctctcg ccaccagcta cgcctacgtt aacccggtgg tcgcggtctt
    18241 gctgggtacg ggactgggtg gagaaacact gtcgaagatt gaatggctgg cgctcggcgt
    18301 aattgtcttc gcggtggtac tggtcacgtt gggaaaatat ctcttcccgg caaaacccgt
    18361 agttgcgcca gttattcagg acgcatcaag cgagtaaatg aatcccctgc gtgtcgatct
    18421 gcgcgctggc accttcgccg cagatccact cttccagacg ctcgctaagc gcttcatccg
    18481 tcagcttctc gcgcccacgt aacgcgcact cccagacaat caatacgcgc cagccgagtt
    18541 cctgcaagcg actgatatcg cggcgatcgc gctcaacatt tttacctatc ttctccagcc
    18601 agaactcggt tcgagtcgca ggcactttaa acagatagca gtgatgatga tgccagaagc
    18661 agccatgggt aaatatcacg cagcgatatt catcaacgac aaaatccggg cgtccgggca
    18721 gactggcgtc ctgaacgcga aacgccaggc cctgcccggt taacagactg gcgaggcgct
    18781 tctcaatcgc cgtatcacgc gtggcaatcg cgcgcatatt tttgctgcga gtggccttat
    18841 cgtgaacgtc ggccatgttg tgcctcttgc tgacgcaacg ccaccgcctg tttgattttt
    18901 ggctcaagca gttttgccac cgcggcaaag accggcacga ccaccgagtt accgaactgg
    18961 cgataggcct gagtgtccga aaccggaatg cggaatttcg cttctcccgg cgcttcaaaa
    19021 cccattaagc gcgcgcattc ccgaggcgtt aaccgacgtg gacgatgttg ctgattcagc
    19081 ggatcgtcaa agtctttctc acccgtggcc atatcccatc cgcgatcgat taaaatttcc
    19141 gcgccatctt tgtaataacg cgcagacagc gtacgggtga cgctttgcgg attgttcgga
    19201 taaaccattc cataaccgaa gccgttaccg cgcgcctgat gttttttcgc atatcgatag
    19261 aggtacttcc acagtaccgg cgtcaggata tatttcgcct cgaccatcgg gtccagcagc
    19321 tgcgccagcg tcactcgctg cgcagggaaa catccgctga tatcacgcag agtaaaatcg
    19381 gctttcaaat taagatcgcg acgaaaaccc accagcacga tgcgttcacg gtgctgcggc
    19441 agaaaatgtt taccatcgat gattttcgga tcgtccggcc cgttatcttc tgcatcagcc
    19501 acgtcatagc ccagttcgtc cagcgtctgc atgatgatgc ggaacgtttt accctggtcg
    19561 tggcttttca ggtttttgac gttttcgagc acaaacatcg ccggacgacg cgcgtcgata
    19621 atgcgcacca catcaaagaa cagcgtgccc tgagtatcgc aggcaaaacc gtgcgcccgc
    19681 ccgagcgagt tctttttcga tacgccagcc agcgaaaatg gctgacaagg gaaaccggcc
    19741 agtaaaacat cgtgttcagg aatgtgttga cgaatatgtt ccgccgccgc ctcatcactc
    19801 acaccttctt tatggctgag ggttatgtcg cggatatctt cattaaaatg atgcgtcgcc
    19861 gggtcgcaat aatggttggc tttataagtg cgtaccgcat gtttgttcca ttcgctggta
    19921 aacacgcatt gtccgccaat cgattcaaaa ccgcgacgga taccgccaat tccggcaaat
    19981 agatcgataa agcgaaacgc ataatgcgga tgatgtgccg gtggtttggg taacaacgtt
    20041 tgcagatggg cgaactcttt ctcacttaaa cggtgccatg ccgagtcatt cgccagcgca
    20101 cgttttaaaa ttgccgcgct ccagtgattc tcacccacac cattaagctg cgccaccagc
    20161 gttttaacat cataaatttg cagcagtttc tccagcattg cctgtgcggc attcccggtg
    20221 ctgtatgaat cggttactga tatattttcc tgcatagatt taaccggcca tctgagatgg
    20281 tcgacaggtt aacataatta cagccattta gggccacgcg ctcagcccgc gggtgaaaac
    20341 gcatctatca ccttgtgatc gacaccttca ttctcccccg ccagttcggc actgagcttc
    20401 gccataaact cgactaaaaa ttgcgcgttg tgctgcgcca gctgcttgcc tcttacggtt
    20461 tgcatggttt gcggtagctt cagcaactta gtctggaaat gatccagcgc gtagcgttta
    20521 tcatcaagcg ggcgatactg tgcgaacgga tcttcgccat caaacagcgc cacgcccaat
    20581 gcccctgaaa cggcaaacac gcgcgccagg ccaatcgccc ccagggcttc cagccgatcc
    20641 gcatcctgga cgattttagc ttccgttgtt aagggggcga tttgcgcgct gaaactgtga
    20701 gcggcaatgg catgacaaac ggcctcgatt ttctccgccg gaaattgctc gaactcttca
    20761 cggagcaggc gacgcgtctc ttctgccgcc aggattgaag aacgctgccg ttgcggatga
    20821 tttttcgcca ggctcacaat atcgtggaaa taacaggcgg ttaaaatcac cagcatatcg
    20881 acgtcatcat ctgccgccag cttctgtgcc gtagcccaga cgcggcgaaa atgacacacg
    20941 tcatgggccg cgtcctggtg ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc
    21001 cagtgttgta agtccatacg cgctccgttg ccaaagagtc gccaccttag cagtttttat
    21061 tcatagcgtc tgctacgtgc gggacgaggt cgggtgtacc aggcaatgct gcctaacaat
    21121 gccccgacca gagccataaa taaaaatccg accagtgccc ctaaccactt tcccgcggta
    21181 gagcctaaat cgctttcaaa tcccgacacg ggggcatcgg ggatccggct catcacccag
    21241 atgtaacgcg ccattgccca gacgaaatag ccacaccatg tatagaaagc ccacaaagcc
    21301 agtttgccgc cagggctgcg agagagtctc tcttccatct ttgtcgaacc gtattccatt
    21361 ttgagataac ccggaaaatt cagacttagt actattatgt gataaaagtc acatttttcc
    21421 acattgaatg agccagtcaa gactcaatca ttttttgaac aaatagataa attttgcgca
    21481 acggcagtga atacaggaaa aatcgttttg agcgatttta tttatctttg atatcggct
//
LOCUS       DAZFFG010000083        21307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFFG010000083 DAZFFG010000000
VERSION     DAZFFG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.083"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            26..262
                     /gene="lpp"
                     /locus_tag="ACHJ7J_003413"
     CDS             26..262
                     /gene="lpp"
                     /locus_tag="ACHJ7J_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO5001600.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(326..1330)
                     /gene="ldtE"
                     /locus_tag="ACHJ7J_003414"
     CDS             complement(326..1330)
                     /gene="ldtE"
                     /locus_tag="ACHJ7J_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO5001601.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1479..1895)
                     /gene="sufE"
                     /locus_tag="ACHJ7J_003415"
     CDS             complement(1479..1895)
                     /gene="sufE"
                     /locus_tag="ACHJ7J_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO5001602.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(1908..3128)
                     /gene="sufS"
                     /locus_tag="ACHJ7J_003416"
     CDS             complement(1908..3128)
                     /gene="sufS"
                     /locus_tag="ACHJ7J_003416"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO5001603.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLEMLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(3125..4396)
                     /gene="sufD"
                     /locus_tag="ACHJ7J_003417"
     CDS             complement(3125..4396)
                     /gene="sufD"
                     /locus_tag="ACHJ7J_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO5001604.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGVKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSILGEVSPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSMLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(4371..5117)
                     /gene="sufC"
                     /locus_tag="ACHJ7J_003418"
     CDS             complement(4371..5117)
                     /gene="sufC"
                     /locus_tag="ACHJ7J_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO5001605.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRNGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(5127..6614)
                     /gene="sufB"
                     /locus_tag="ACHJ7J_003419"
     CDS             complement(5127..6614)
                     /gene="sufB"
                     /locus_tag="ACHJ7J_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO5001606.1"
                     /translation="MSRNTEAIDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(6623..6991)
                     /gene="sufA"
                     /locus_tag="ACHJ7J_003420"
     CDS             complement(6623..6991)
                     /gene="sufA"
                     /locus_tag="ACHJ7J_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO5001607.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(7540..7728)
                     /gene="ydiH"
                     /locus_tag="ACHJ7J_003421"
     CDS             complement(7540..7728)
                     /gene="ydiH"
                     /locus_tag="ACHJ7J_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO5001608.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(7828..8238)
                     /gene="menI"
                     /locus_tag="ACHJ7J_003422"
     CDS             complement(7828..8238)
                     /gene="menI"
                     /locus_tag="ACHJ7J_003422"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO5001609.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(8235..11291)
                     /gene="ydiJ"
                     /locus_tag="ACHJ7J_003423"
     CDS             complement(8235..11291)
                     /gene="ydiJ"
                     /locus_tag="ACHJ7J_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO5001610.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLMR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            11680..12792
                     /gene="ydiK"
                     /locus_tag="ACHJ7J_003424"
     CDS             11680..12792
                     /gene="ydiK"
                     /locus_tag="ACHJ7J_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO5001611.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            12978..13085
                     /gene="rprA"
                     /locus_tag="ACHJ7J_003425"
     ncRNA           12978..13085
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHJ7J_003425"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            13221..13577
                     /gene="ydiL"
                     /locus_tag="ACHJ7J_003426"
     CDS             13221..13577
                     /gene="ydiL"
                     /locus_tag="ACHJ7J_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO5001612.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            13677..14891
                     /gene="ydiM"
                     /locus_tag="ACHJ7J_003427"
     CDS             13677..14891
                     /gene="ydiM"
                     /locus_tag="ACHJ7J_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5001613.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            15118..16383
                     /gene="ydiN"
                     /locus_tag="ACHJ7J_003428"
     CDS             15118..16383
                     /gene="ydiN"
                     /locus_tag="ACHJ7J_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5001614.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPNQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            16395..17261
                     /gene="ydiB"
                     /locus_tag="ACHJ7J_003429"
     CDS             16395..17261
                     /gene="ydiB"
                     /locus_tag="ACHJ7J_003429"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO5001615.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            17292..18050
                     /gene="aroD"
                     /locus_tag="ACHJ7J_003430"
     CDS             17292..18050
                     /gene="aroD"
                     /locus_tag="ACHJ7J_003430"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO5001616.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18196..19791
                     /gene="ydiF"
                     /locus_tag="ACHJ7J_003431"
     CDS             18196..19791
                     /gene="ydiF"
                     /locus_tag="ACHJ7J_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO5001617.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            19805..20956
                     /gene="ydiO"
                     /locus_tag="ACHJ7J_003432"
     CDS             19805..20956
                     /gene="ydiO"
                     /locus_tag="ACHJ7J_003432"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO5001618.1"
                     /translation="MDFCLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(20999..>21307)
                     /locus_tag="ACHJ7J_003433"
     CDS             complement(20999..>21307)
                     /locus_tag="ACHJ7J_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5001619.1"
                     /translation="LSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWLLTNTE
                     LSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG
                     "
BASE COUNT         5196 a         5395 c         5184 g         5532 t
ORIGIN      
        1 taactcaatc tagagggtat taataatgaa agctactaaa ctggtactgg gcgcggtaat
       61 cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc agctgtcttc
      121 tgacgttcag actctgaacg ctaaagttga ccagctgagc aacgacgtga acgcaatgcg
      181 ttccgacgtt caggctgcta aagatgacgc agctcgtgct aaccagcgtc tggacaacat
      241 ggctactaaa taccgcaagt aatagtacct gtgaagtgaa aaatggcgca cattgtgcgc
      301 catttttttt gtctgctatt taccactact gcgtcgcgcg taacatattc ccttgctctg
      361 gttcaccatt ctgcgctgac tctactgaag gcgcattgct ggctgtggga gttgctccac
      421 tgctcaccgc aaccggatac cctgcccgac gatacaacgc tttatcgact aacttctgat
      481 ctacagcctt attgtcttta aattgcgtaa agcctgctgg cagtgtgtat ggcattgtct
      541 gaacgttctg ctgttcttct gccgatagtg gtcgatgtac ttcaacataa cgcatcccgt
      601 taggctccac ggaatatttc accggttcgt tgatcacttt caccggcgtt cccgtccgca
      661 cactggagaa caaggcttta atatccggtg cattcatgcg aatacaccct gaactgacgc
      721 gcaaaccgac gctgtccggc gcactggtgc catgaatgag gtattcgcca ttaccatgcg
      781 cgaggcgcag tgcgtaacgt cctagcgggt tatttggtcc ggcaggaacg actggcggta
      841 atttaatgcc acgctccagc gaacgttgac gaatgcctgc agtaggcgtc caggttgggt
      901 tagggatttt ctgccccaca cgcgtttcca tcaccggcgt ttccagcccc tgcaagccaa
      961 tacctatcgg ataaacctgc acgatatttt ctcccggcgg ataataataa aggcgcagct
     1021 ctgcaaggtt aacgataatc ccctgacgcg gcgcatcagg taataacagt tgcgaaggaa
     1081 tagttatcgt cgtaccgggt tttggcaccg gggcgatagt gttattggct tcaaggatca
     1141 acattgccgc agtatcaaaa cgtcgggcga tggcctgaag gtttttatcc ccttcctgca
     1201 ccgtatacgt ttgattttgc ccaaccagtc ggcttccggt tggtggtagc ggataatcaa
     1261 ccgcccaggc agcctggatg gcgctaaaag cgccgataag cgtgagtgta agcaaagacg
     1321 cgcgtttcat tgtaaacctc ctgtatttgc cggagactca cgctgaaacg ttggatggcg
     1381 cttatgttca cctgaaaccg aaacactcct gtgcaggtca gtgtaaacat tgaccatccg
     1441 gcaatgtgag ccaaccggat gaaagctgtc cctttagttt agctaagtgc agcggctttg
     1501 gcgcgaattg cgcgaatcat cgcttccaga ccttgtgaac gagatggggt gagatgttgg
     1561 gtaagcgcca ttttttcaaa ccacggacgc acatcgaaat tgacaatatc ctgcggcgtc
     1621 atctgatcgt agagaataaa gacgaccgca ataagccctt tcacaatcgc cgcatcgctg
     1681 tcgccctgca attcaataat tccctgcgca ttctggcgca tgacaatcca cacctgactc
     1741 tggcagccct gaatgctatt ttgtgggctt ctgtcttcgt cgcgtaattc tggcagacgc
     1801 tggcccagct caataatgta gagatatttc tcttcccagt tggcgcagcg taaaaaatta
     1861 cgcagcaatt tttctttatc cggcaataca gccatagtgc ctccctgtta tcccagcaga
     1921 cggtgaatac gttgcaatcc ggtcaccaga cgatccactt cttcatgggt gttatacatg
     1981 gccagcgacg cccgacacat cgcagggacg ttataatagg ccattaatgg catagcgcag
     2041 tgatgtccgg tacgcacagc aatgccgtaa ttatcgagaa aactgccaac atcataggcg
     2101 tggtgtttac cgagattaaa agcaataacg ccaagcctgt tttgtgggcc atagagagtg
     2161 agatccggta cagattccag ctgtgatagc gcgtaatgca tcagattctg ttcatactcg
     2221 gctatgttat taagccccag cgccgaaaca tactccagcg ccgcgccaag accaatgatg
     2281 cccccggtat tgggtgtacc ggcttcaaac cgccatggtg ctttggtcca ggtagtgcct
     2341 tcactcaggc tgacggtggc gatcatagaa ccgccccctt cccacggcgg catctcctgc
     2401 aacaaggctt ctttcacata aagaatgcca attccggtgg ggccatacag tttatgcccg
     2461 gagaacacgt aaaagtcgca atccagcgcc tgaacatcca ccggatgatg catcaccgcc
     2521 tgagcgccat ccaccagcac ttttgcgcca tgctggtgcg caagcgtgat catttccgcc
     2581 agtggatttt ctgtgccaag cacgttggag acatgagtaa ttgccagcag gcgagttttc
     2641 tcatcgaaca gattaggtag catctccagt tgcaacgtac cgtcgggatt gagcgggatc
     2701 acacgcagct ctgcgccaac gcgtgcgcaa agcatctgcc atggaacaat gttagcgtgg
     2761 tgctccatct gacttatgat gatgttatcg cccgcccgca cgttgctgtt tccccagcta
     2821 ttggcgacca gattgatccc ttccgtcgtg ccgcggacga acaccagctc ttccgccgaa
     2881 cgggcattaa taaacagcga tgcccgcttg cgcacgttct ccattttctc ggtcgcctgg
     2941 gcgcttaagg tatgaatacc gcgatgcacc gccgcgtagc catgacgata aaactcggcc
     3001 tcggcgtcaa tcacctggct cggtttctgc gcactggcgg cgctgtcgag ataagccagc
     3061 ggcaaaccgt ttacctcacg cgaaagcacc ggaaagtcgg cccgcacttt gtcgacggaa
     3121 aaagtcatct tgcacctcct ggcagccgtt gaccgattcg ggccagcacc tgctgtttaa
     3181 gcccctcatc acgcagtgct tccgtcagtt cggcagcaaa ggcgtaaatg atcatctgct
     3241 gggcatcctg ctgattgatc ccgcgcgagc gcagatagaa catctgttca tcatcaatac
     3301 gccccaccgt cgcgccgtgg ctgcatttca catcatctgc atagatttcc agctgcggtt
     3361 tcgtatccac ttccgccagt ttgcccatca gcagattatt gttggtcatc tgaccatccg
     3421 ttttgatggc gtgctgcgcg acgttgatca aaccgttaaa taccgcgcgg cctttgtcgc
     3481 tgacgatagt tttgtgcaac tgtcggctgt tacaaaaacc tttattgtgt tccagccagg
     3541 tacgggtatc acacacctcg tttttcaccg gcatcgccag gctattgatc cgcaacatgc
     3601 tgttttcgcc attgagttgc gtactggtgt tgtgtcgtaa cactgcgcca cccagcagga
     3661 aactgtggct aaatgcggtg gcatcgtcag ccagcaacaa atcgttatgg gcaaagtggt
     3721 gactgagcgg gttttcaaac gccagcttga tatgctgcaa gtgggcattc gctgcgacat
     3781 tgatagtgaa ccgtgccccg gtaaaatgac gagcatcatt aaggctgaca aaatgttcga
     3841 tcaccgttgc ttcggcacct tccgccagat ccagatgatg tcggtaatgg gcagtgttca
     3901 cctcttcacc tgccacgccc tgggtgatat gcattaacag caatggcttt gccggccgtt
     3961 gaccgcgctt cacggcgata tgcgtcacgc tttgtgccag gctttccgtc aaatgcagaa
     4021 acacttccgc ctgaatagcg tcgggtaacc cctgacggtc gtcgttaatg ctcacttcat
     4081 atccgctgcc ttcagttgca tcgctcagcg caggcacgta acgtccatcg acaaacacca
     4141 gccgcacggc gtctaacgtt aacgctaagg catcacgctg ctgtggggat acctccccca
     4201 gaatgctgac aaactggcta ttggtcagcc cttccagcgg cgtatatttc cagttttcat
     4261 gtttacgtgt cggcagtccg gtacgcagca attgttgtaa atgctgttgt gcttgcgggg
     4321 aacgtttcac cccttcagct tcaaacaaat gatgccactg ttgcagtgcg ttactgctgt
     4381 tcggtaagcc agccataacc ctgctcctcc agttgtttga ccaacgtgaa atcgccggat
     4441 ttcacaattc gtccctgata tagcacatga acgtaatcag gcttgatgta gtcgagaatg
     4501 cgttggtagt gcgtaacaat gatgaatgaa cgcttgccgt tacgcagcga gttcacgcca
     4561 tcggcaacca cttttaatgc gtcgatatcc agcccggagt ccgattcatc aagaatgcat
     4621 aactccggtt ccagcaccgc catttgcaaa atatcgttgc gctttttctc gccgccggaa
     4681 aaaccaacgt ttaccgaacg ggttaataaa tcttccggca tcttcaggag agcgattttc
     4741 tcttccatca aatcctgaaa atcaaagcgg tcgagcgttt cctggccgcg atagctgcgc
     4801 accgcattaa gtgccgtttg caggaaaaac tggttactga cacctggaat ctccaccgga
     4861 tactggaagg ccataaagat gccttcgcct gcacgatctt ccggcgacag cgcaagcaaa
     4921 tctttgcctt tgaactcaac cgtgccgccc gtcacttcat aatcttcgcg cccggcaagc
     4981 gttgccgata aggtactttt gcccgaaccg tttggcccca taatggcgtg aacttcgccg
     5041 ggacgaacgt cgaggcttaa tccgcgcaga atagctttat cttccacgct gacgtgtaaa
     5101 tctttaatac ttaacatgtt tattccttat ccgacgctgt gttcaagact gatggcgagg
     5161 agtttttgtg cttcaacggc aaattccagc ggcagttccg agaacacgtc tttgcagaaa
     5221 ccgttaacaa tcatcgagat ggcgtcttct tcgctgatcc cgcgctgcag gcagtaaaac
     5281 agttgatctt caccaatacg tgatgttgtt gcctcgtgct ccagttgtgc gctgttattg
     5341 cgacattcga catacgggaa ggtatgcgcc ccacaattag cgccaatcag cattgagtcg
     5401 cactgagtga aattgcgcgc attggttgcc gtcggcatga ttttcactaa gccgcgataa
     5461 ctgttctgac tatgtccggc agagatccct ttcgagataa tggtcgattt ggtgttttta
     5521 ccgatgtgga tcatcttggt gccggtatca gcttgctgat gaccgctggt cagcgccact
     5581 gagtaaaact caccaatgga gttatcgccg cgcaaaatgc agctgggata tttccacgta
     5641 atcgctgacc cggtttctga ttgcgtccat gacattttgc tgttttcgcc ttcgcacaaa
     5701 gcacgcttgg tgacgaagtt gagaataccg ccggtgttgt tatcgccagg gaaccagttt
     5761 tgtaccgtgg aatatttcac ctcggcgttt ttatggatga tgacttccac cactgccgcg
     5821 tgcaactgat agctgtcacg caccggagcg gaacagcctt caatgtagct gacgtagctg
     5881 tcttcgtcgg ccaccagaat ggtgcgctca aactgcccgg ttttttccgc gttaatgcga
     5941 aaataggtgg aaagttccat cgggcagcgc acgcctttag gcacataaat aaacgtaccg
     6001 tcagaggcta ccgccgcatt aagcgcggca aagaagttgt cattccccgg caccacggtg
     6061 ccgagatatt tacgcaccag ttccgggtga tcgtggatcg cctcaccaaa ggaacagaaa
     6121 ataattccct gctccgccag tttttcgcga taagtagtgg caaccgaaac tgagtcgaaa
     6181 atggcatcca ccgccacctc tttgccttcc cgcacgggaa cgcccaactg ctcaaacgcc
     6241 gcctccacct ctttacttaa aaaggcgttc gcgccagttt gctgcaccgc gccaggttca
     6301 gacgcgcaag tgtcgtcaca attaccgcac gatggtgctg agtagtagct gtaatcctga
     6361 taattcagct tgtcgtagtg cgctttcaac cagtgcggtt cttccatctc cagccatgcg
     6421 cgataggcgt ttagacgaaa ctccagcatc cactccggct cattacgctt cgccgaaatt
     6481 gcgcgcacca cctcttcgtt tatccccttt gccagctcat cggtggctaa ctgggtgaag
     6541 aatccttctt tataattcag cgggccgccg gtccaggttt tgacatcgtc aattgcttca
     6601 gtattacgag acatagtacc gcctataccc caaagctttc gccacagcca cattcattct
     6661 gggctttagg gttgtgaaat ttgaatatct gattaagtcc ttcacgaacg aaatcgactt
     6721 ccgtgccatc aataaacggc atcgcttgca gcgggacaaa cagcttcgcg ccgtcgtgtt
     6781 caaacagcag atcgtctttg tccggctcgc taacactgtc gagcacatag ccaaagcccg
     6841 cgcagcccgt ttgcttcaca cctaagcgca cgccgaccat acctggctgc tttgccacca
     6901 gctcgcggat gtgtatcgcc gctgcgggtg tcagcgttaa gccttgccag gcgaaatcct
     6961 gtgggttaaa ggttcctgaa tgcatgtcca tctatttacc tcacttcatc gctttcagcg
     7021 tataacagca tgttagtgat aatgattatc agttcaaccc agcaaacgca ggggctttac
     7081 cgtaaaacat gctttttgcc tgcttttaat aagcatagac cctgatgtgt gggttaacag
     7141 gcacgctaag aattaggtat ctcattgtta gataatggtt attatctaag gagcattaag
     7201 gctgtgatga aaaagaaaaa gttgtattga aaatgactat ttaagagata ggtaaaaaag
     7261 tgcagcgttc agaaataaga aaacccttaa gtctgtgcga cacaggctta agggtttcta
     7321 ccccatccgg cgcttatctc cggcactctc agtggcttag ctcttgaagg ggcgataaga
     7381 ataatctcat aaagctaacc cgccgtttta acacaaactg cgattagtat catttttgaa
     7441 caatatcagg cgggtagata agcagtatta agaaggtcat cgaacctgga cggaggttaa
     7501 tccaggtcga tttggcgaac ttgcggtatt aagtcaggat caatgcacgc ccagacgcca
     7561 ggcaaagtag atttcttctt ttaattcagc agaagagaga gtaagcaggt cagcaaattc
     7621 aagttctagt tgtttcagac gtttgagata ttgggcaggt gaaagattgc tctggtcacg
     7681 gcgtaaaaat tcaatggcca gctgggtggg atcaagttga gtagacatag catcctcgct
     7741 tttagacaag acctgcacag tataccaccg tttactgtgc agataatgac caaaagcaat
     7801 atgcgtcaca cttttctggt gacaacgtca caaaatggcg gtcgtcaatc gtgacgaaca
     7861 gcacaaacgc cctttctcat cgaagatttc aatctgccag acctggtgac gcgaaccgag
     7921 atgcaacggt ttgcatacgc cgcgcacccg cccttctcgt gccgagcgga cgtggttagc
     7981 attgatttcc agaccaacca ctttttgctc accttcggta cataaataac cggcaacgga
     8041 accgatactt tcggccagta ccacggatgc acctccatgt agcaacccga aaggctgctt
     8101 tgtccgcgag tctactggca ttgtcgcttc aagggtgtca tcaccaatat gttcaaagcg
     8161 aatatccagt aaccccacca tgtttccttc acccatggca ttcagtgctt ccagggtgat
     8221 ttttcgtttc catatcattt aataatctcc agtaaagcct gcacaggatg gcgtaccccc
     8281 gtgccttcaa cccgtttgac ctgactacgg caggaatatc cggtcgccag acagcggtta
     8341 cgcggcagtc gctgcatagc ctgatgccag gataactcat agatcccgag cgaattttta
     8401 tggtttttcg cttcatgtcc gtaagtccct gccatgccgc agcaacccac gctgacattt
     8461 tccagtttcg cgccaaaacg ggcaaatatc gcggcccatt gtgctggcgc acccggcaag
     8521 gcggtaactt cggtacagtg accaaagaaa taccatgatt caccgctgac tgtagccact
     8581 ggctgtgact caagtgcgct tgccagccat tcattcgcca gtaagacgtt aaactcgcca
     8641 cgttcctcgc ccagtgccag tttatattca tcgcgataac aaagtaccag cgccggatcg
     8701 acgcctacca ttggcatacc cagcttcgcc atacggttga ggaaatccgc cgtctttttc
     8761 gccgtcttcg caaaacgatt aagaaaacct ttaatatgct gggctttgcc atttggcgaa
     8821 aatggcagta acacaggctg gaaacctaat ttttcgacca gacggacaaa atccgccacc
     8881 acttgcgcat cgtaatagct ggtaaagggg tcctgcacca ccaacaccgt gcgcgctttc
     8941 tgctctgcat tgagagcttc aagctgttcc agcgtcatgt ttgccgagcg atgccccacc
     9001 atttgttgtt gtagcgaggg gaccgacagc agcggcaaat caaccatgcc gatatgtttt
     9061 tccgagagtt tgcgcatcag cggctggtta atgaagaagt taaaggtttt cggcgcgcgt
     9121 gccatcagcg gtgcgtagct ctcgaccgta gcgacgaggt ggtcgcgcag cggacgtaaa
     9181 taacgggtgt gatagagctg caggaaacga gagcgaaact ccggcacatc aattttgatg
     9241 gggcactggg tcgaacaagc tttacaggcc agacagcccg acatcgcctc tttgacttcg
     9301 tgtgagaagt catattcgcc tttattcgca tgccagctat tgcgcgtgcg ggcaattaac
     9361 gtccgcaaac tgacgccgga ttcaggcagt tcttgttcca gtttgagtgg atcaacgccg
     9421 cgatccgcca acaaacgcag ccattcacgc accagcgttg cgcgcccttt cggtgaatga
     9481 atccggttct gggtgatctt catcgacgga cacatcggac tacgggcatc aaagttgaag
     9541 cataaaccgt tgccgttaca ctccatcgca ccgcgccact gctggcgtac cgcaatgggg
     9601 atctgccgat cgaatgtacc gcgcttcacc gcgtccactt tcatcatcgg cgcatcgaga
     9661 ccttctggtg ggcaaatctt ccctgggttg agtctgttat gcgggtcaaa tgccgctttc
     9721 actttgcgca gttctgcaaa aagttcctcc ccaaaaaacg ccgggctgta ttcggcacga
     9781 aaaccttttc cgtgttcgcc ccacaacaaa ccaccgtatt tcgcagtcag cgcaaccacg
     9841 tcatcagaga tttgcttcat caaaatctct tgttgtggat cgcacatatc cagcgccgga
     9901 cggacgtgca acaccccagc atcgacatga ccaaacatgc cataacttaa gccgtggctg
     9961 tcgagcagcg cgcgaaattc agcaatataa tcggccagat gttccggcgg tacgcaggta
    10021 tcctcagcaa acggaattgg cttagcggca cctttggcat tgccaagcag accaacggct
    10081 tttttgcgca ttgcatagat acgttcaacc cccgccagct cacggcacac ctgccagccg
    10141 atgacacctg cttgatgact ggcgatcagc tcatccaacc gcgcacagag tgcatttact
    10201 cgctcatcaa tcagcgcctc atcatcacca gcaaattcca caatgttcag cccgagcatc
    10261 tctttgtcag gcacatcggt aatcaactcg ctgacggaat gccagacaat atcttcccgc
    10321 gccagattca gcacttttga gtccaccgtc tctaccgaaa gcgcccgcgc ctcaaccata
    10381 aacggcgcgt tacgcagcgc agagtcaaaa gagtcatatt tgacattcac cagacggcgc
    10441 actttaggca agcgcgtaat atccagccgc gcttcggtaa taaaggccag cgtcccttct
    10501 gaacccgtca gaatgcgcgt caggtcgaac tcggtcatct catcgttaaa gacatgacgc
    10561 agatcgtaac cggtaagaaa gcggttaagt ttggggaagt tgtcgataat taactggcgt
    10621 tgctgacggc aacgttgata aaccgtgtta taaattcgcc cgattgtggt attggattta
    10681 cccagcgttt ccgccagctc gacgggtaaa ggttgcgtat cgagaatatc gccccccaac
    10741 aataccgcac gtaggccaag tacgtgatct gacgttttgc catagaccag cgatccctga
    10801 ccggatgcat cggtattgat catcccaccg agcgttgccc ggttgctggt cgaaagttcc
    10861 ggcgcaaaaa agtagccgaa cggtttcagg tactgattga gttgatcttt tatcaccccg
    10921 gcctcaacgc gcacccagcc ctcttcaggg ttaatttcga tgatgcggtt catatggcgg
    10981 gacatatcaa caataatgcc ctggttgagc gcctgaccgt tagtgccggt gccgccgccg
    11041 cggggggtaa agatcagcga tgaatagcgt tcctgcgcgg caagacgggc gatcagcgcc
    11101 acatctgcgg ttgaacgcgg aaataccacc gcatcgggga gaagttggta aatactgttg
    11161 tcggtcgaca ttgtcagacg atcggcataa cttgtcgccg tatcgccggt aaaaccttgt
    11221 tgctccagct cttgcaaaaa attaagcacc agttgaacga cgccaggtgc ctgggaaatc
    11281 tgtggaatca ttatattgac cctttcctgc ggtctgtgat gtaggtcgat acactgttct
    11341 ttcaggcggc tgcaatagcg cacagaaagg tgatgtttat ttactctatg gatttcgagt
    11401 tgcaggaagg cggcaagcga gtgaactcca ggagcttaca acagtaagta actggggtga
    11461 acgaacgtag ccgcagcata tgcaacttga aatacgacga gtaaatcgtt tgcgtgttgc
    11521 ctgagttgtt gtaccacatt tttttctaac acgcccatca gaattaaggg cagaatcggc
    11581 ctgttaaaaa ccgctgaaat tgctcatcat tatgcaggtg agtttcgcgt gttcacgtcg
    11641 cgtcgacgat ttgacgcaca aaaaaggtga aaagtagtta tggtaaatgt tcgtcagccc
    11701 agggatgtcg cacaaattct gctttcggtg ctgtttttag ccatcatgat tgtggcatgt
    11761 ctgtggattg ttcaaccctt tattctcggc tttgcatggg ccggtacggt ggttatcgcc
    11821 acctggccgg tattgttacg tttgcaaaag atcatgtttg gccgccgctc tctcgccgtt
    11881 ctggtgatga cgctgttatt agtgatggtg tttatcatcc ctattgcttt gctggttaac
    11941 agtatcgtcg acggcagcgg cccgctaatt aaagccattt ccagcggtga catgacgtta
    12001 cccgatctgg cgtggcttaa taccattccg gtgattggcg cgaagctgta tgcaggctgg
    12061 cacaacttgc tggatatggg ggggacggcg atcatggcga aagtccgccc ttatattggc
    12121 accaccacca cctggttcgt tgggcaagcg gcgcatatcg ggcgctttat ggtgcattgt
    12181 gcgctgatgc ttctcttcag tgccctgctg tactggcgcg gtgaacaggt ggcacaaggc
    12241 attcgccatt ttgcgacccg tctggcaggc gttcgcggag atgccgccgt actgctggcg
    12301 gcacaggcta tccgcgcggt ggcgctgggt gtggtggtga cggcgttagt acaggcagtg
    12361 cttggcggta tcggcctcgc cgtatccggc gtaccttatg caactttgct aacggtgtta
    12421 atgatcctct cctgccttgt ccagcttggc ccgttgccgg tactgattcc ggcgattatc
    12481 tggctctact ggaccggcga taccacctgg ggaacggtat tgttagtgtg gagcggtgtg
    12541 gttggcacgc tggataacgt catccgccca atgttaattc gcatgggtgc cgatttaccg
    12601 ctgatcctga tcctctctgg cgttattggt ggtttgattg ctttcgggat gatcggtctg
    12661 tttattggtc cggttctgtt agccgtttcc tggcgtctgt ttgccgcgtg ggtggaagaa
    12721 gtcccgccgc cgacggacca accggaagaa attctcgaag aacttggcga aatcgagaaa
    12781 ccgaataagt aatttctcat caggcggctc tgccgcctga ttgttaaccc ccgctaattg
    12841 atatttgctt gctcttcccc atattttcct gcttaccgcc attcagctgg tagtacctgt
    12901 cgcaaattct ttacagtttt taaactaatg agacgaatct gatcgacgca aaaagtccgt
    12961 atgcctacta ttagctcacg gttataaatc aacatattga tttataagca tggaaatccc
    13021 ctgagtgaaa caacgaattg ctgtgtgtag tctttgccca tctcccacga tgggcttttt
    13081 tttaacattt ttccgcttcg ctacctcgcc cctcactctt tccaataatg ttgcacaaga
    13141 tactgtgact ctcttcaagc cgtcagcagg ctgataatgt tatttatatt ttattgttta
    13201 tttcatgcag ccattacacg atgaacgctt atgaactcca ggcactccgc catatttttg
    13261 ccatgactat tgatgaatgc gcgacctgga ttgcccagac cggtgacagt gaaagctggc
    13321 gtcaatggga aaatggcagg tgtgccattc ctgattgtgt agtcgagcaa ctgttggcta
    13381 tgcgtcagca aagaaaaaaa catcttcatg ccatcataga gaaaataaat aaccgcatcg
    13441 gcaacaacac catgcgtttt ttccccgact taaccgcgtt tcaacaagtc taccctgatg
    13501 gcaattttat tgactggaag atctatcaat cggtagccgc tgaattatat gcacacgatc
    13561 tggagcggct ttgttaattt ctccacaaaa aggaattgtc gttgttacaa caataatgaa
    13621 cggatactga cacaacatcg ctacactttt taaagcacct ttgctaagta gaacctatga
    13681 aaaatcccta ttaccctacc gcacttgggt tgtattttaa ttacctggtg catggtatgg
    13741 gcgtcatttt gatgagcctg aatatggcct cgctggagac actttggcag actaatgccg
    13801 cgggtgtctc gatagttatc tcatcgctgg gcattggtcg attaagtgtc ttgctttttg
    13861 caggattatt atccgatcgc tttggtcgcc gcccttttat catgctaggg atgtgctgct
    13921 atatggcctt cttttttggc atcctgcaga ccaataacat cattatcgct tatgtttttg
    13981 gctttctggc gggaatggca aacagttttc tcgatgcagg cacttatccc agtttgatgg
    14041 aagcttttcc acgctcacct gggacagcca atattttaat taaagcattt gtttccagcg
    14101 gacaattttt attaccgcta atcattagcc tgttagtgtg ggctgaactg tggttcggtt
    14161 ggtcctttat gattgctgca ggaattatgt ttattaacgc tctgttttta taccgttgta
    14221 cgttcccacc ccatccgggt cgtcgcttac ctgtcataaa gaaaaccacc agctctacgg
    14281 aacatcgctg ttcaattatc gatttagcca gttatacctt atatggctat atttcaatgg
    14341 caacgtttta tctggttagc cagtggctgg cacagtacgg acaatttgtt gcaggcatgt
    14401 catacactat gtcgatcaaa ctactcagta tctacaccgt gggttcgctg ctttgtgtat
    14461 ttattaccgc tccactcatt cgtaataccg ttcgcccaac aacattactg atgctgtaca
    14521 cctttatctc atttattgct ctgtttaccg tctgcctgca tcccacattt tatgtggtga
    14581 taatatttgc ttttgtcatt ggttttacct ctgctggagg tgttgtgcaa attggcctga
    14641 cgttaatggc tgaacgtttc ccctacgcta aaggtaaagc tacagggatc tattacagtg
    14701 cgggcagtat tgcgaccttt actattccgt tgattacggc tcatctgtcc caaagaagta
    14761 ttgccgatat tatgtggttc gataccgcca tcgctgccat cggcttttta ctggcactgt
    14821 ttatcggctt acgcagccgc aaaaaaacgc ggcatcactc gctaaaggaa aatgtcgctc
    14881 cgggtgggta atgcaatatt cttttcaggt catgcaagat cttacggata aataactctt
    14941 tctgcgctaa ctaaggaaaa tcgcgatcaa aaacaaacta tgacatgcaa tattcctgga
    15001 aacataaact ttatgccatg tacccaggga aaatcatctt cagtatagta attatgtaaa
    15061 ccgtcggaga acaatacgta cggtaacgaa attatctttc agcaaggagc tgtgaaaatg
    15121 tctcaaaata aggctttcag cacgccattt atcctggctg ttctttgtat ttacttcagc
    15181 tacttcctgc acggcattag tgttattacg cttgcccaaa atatgtcatc tctggcggaa
    15241 aagttttcca ctgacaacgc gggcattgcc tacttaattt ccggtatcgg tttggggcga
    15301 ttgatcagta ttttattctt cggtgtgatc tccgataagt ttggtcgtcg ggcggtgata
    15361 ttaatggcag taataatgta tctgctattc ttctttggta ttcccgcttg cccgaattta
    15421 actctcgcct actgtctggc agtgtgcgta ggtatcgcta actcagcgct ggatacgggt
    15481 ggctatcccg cgctcatgga atgctttccg aaagcctctg gttcggcggt catactggtt
    15541 aaagcgatgg tgtcattcgg acaaatgttc tacccaatgc tggtgagcta tatgttgctc
    15601 aataatatct ggtacggcta tgggctgatt attccgggta ttctgtttgt actgatcacg
    15661 ctgatgctat tgaaaagcaa attccccaac cagttggtgg acgccagcgt agctaatgaa
    15721 ttaccgcaaa tgaacagcaa accgttagtc tggctggaag gtgtttcatc ggtactgttc
    15781 ggtgtagctg cattctcgac cttttatgtg attgtggtgt ggatgcccaa atatgcgatg
    15841 gcttttgctg gtatgtcaga agctgaggca ttaaaaacca tctcttatta cagtatgggc
    15901 tcgttggtct gtgtctttat ttttgccgca ctactgaaaa aaatggtccg gcccatctgg
    15961 gctaatgtat ttaactctgc actggcaaca ataacagcag ccattatcta cctgtaccct
    16021 tctccactgg tgtgtaatgc cggcgccttt gttatcggtt tctcagcagc tggcggcatt
    16081 ttacagctcg gtgtttcggt catgtcagag tttttcccaa aaagcaaagc caaagtcacc
    16141 agtatttata tgatgatggg tggactggct aactttgtta ttccgctgat taccggttat
    16201 ctgtcgaaca tcggcctgca atatatcatt gttctcgatt ttactttcgc gctgctggcc
    16261 ctgattaccg caattattgt ttttatccgc tattaccgcg tttttattat tcctgagaat
    16321 gatgtgcggt ttggcgagcg caaatttagc acccggttaa acacaattaa gcatagaggt
    16381 taaaggagtt aattatggat gttaccgcaa aatacgaatt gattgggttg atggcttatc
    16441 ctatccgcca cagtttatcg cctgaaatgc agaataaagc cttagaaaaa gcgggattgc
    16501 catttaccta tatggccttc gaagtggata acgatagctt tcccggagca attgaaggat
    16561 taaaagctct aaaaatgcgc ggaactggtg tatcaatgcc gaacaaacaa ctggcgtgtg
    16621 aatatgttga tgaattaacg ccagcagcca aactggtggg tgccatcaac accatcgtta
    16681 atgatgatgg ctacctgcgc ggctataaca ccgacggcac gggccatatt cgcgccatta
    16741 aagagagcgg ttttgatatc aaaggcaaaa caatggtgct gttaggggcc ggaggtgcct
    16801 caacggcgat tggcgcgcag ggggcaattg aaggtttaaa agaaattaaa ctctttaacc
    16861 gtcgggatga gttcttcgat aaagccctcg ccttcgcgca gcgggttaac gaaaacaccg
    16921 attgtgtcgt cacggttacc gatctcgccg atcagcaagc ctttgctgaa gccctggctt
    16981 ccgccgacat tttaaccaat ggcacaaaag tgggtatgaa accccttgag aatgaatcat
    17041 tagttaatga tatcagtctg ttacatccgg gactgctggt cactgaatgc gtgtataacc
    17101 cgcatatgac gaagttattg cagcaggcgc aacaagctgg ttgcaaaacg attgatggat
    17161 acggcatgtt gttgtggcaa ggggctgaac agttcacgtt atggactggc aaagatttcc
    17221 ctctggaata tgttaaacag gtcatggggt tcggtgcctg acaggctgac cgcgtgcaga
    17281 aagggtaaaa aatgaaaacc gtaactgtaa aagatctcgt cattggtacg ggcgcaccta
    17341 aaatcatcgt ctcgctgatg gcgaaagata tcgcccgcgt gaaatccgaa gctctcgcct
    17401 atcgtgaagc ggactttgat attctggaat ggcgtgtgga ccactttgcc gacctctcca
    17461 atgtggagtc tgtcatggcg gcggcaaaaa ttctccgtga aaccatgcca gaaaaaccgc
    17521 tgctgtttac cttccgcagt gccaaagaag gcggcgagca ggcgatttcc accgaggctt
    17581 atattgctct caatcgtgca gccatcgaca gcggcctggt tgatatgatc gatctggagt
    17641 tatttaccgg cgatgatcag gttaaagaaa ccgtcgccta cgcccacgcg catgatgtga
    17701 aagttgtcat gtccaaccat gacttccata aaacgccgga agccgaagaa atcattgccc
    17761 gtctgcgcaa aatgcagtcc ttcgacgccg atattcctaa gattgcgctg atgccgcaaa
    17821 gtaccagcga tgtgctgacg ttgcttgccg cgaccctgga gatgcaggag cagtatgccg
    17881 atcgtccaat catcacgatg tcaatggcaa aaactggcgt aatttctcgt ctggctggtg
    17941 aagtatttgg gtcggcggca acttttggtg cggtaaaaaa agcctctgcg ccagggcaaa
    18001 tctcggtaaa tgatttgcgc acagtattaa ctattttaca tcaggcataa gcaataatat
    18061 ttcggcggga atatcctccc ccgccgaact aaaaaaaata tattcaaccg tatttaataa
    18121 aaatatttcg tgagtctctg cacgctaatt atccatctgg cgtagggaaa atcacacctg
    18181 aatcaggaat taacaatgaa acctgtaaaa ccacctcgta ttaatggacg agtgccggtc
    18241 ctgtcggcac aggaagcggt gaattatatt cccgacgaag caacactttg tgtgttaggc
    18301 gctggcggcg gtattctgga agccaccacg ttaattactg ctcttgctga taaatataaa
    18361 cagactcaaa cgccacgtaa tttatcgatt attagtccaa cagggcttgg cgatcgcgcc
    18421 gatcgtggta ttagtcctct ggcgcaagaa ggtctggtga aatgggcatt atgtggtcac
    18481 tggggacaat cgccgcgtat ttctgaactc gcagaacaaa ataaaattat tgcttataac
    18541 tacccacaag gtgtacttac acaaacctta cgcgccgccg cagcccacca gcctggtatt
    18601 attagtgata ttggcatcgg gacatttgtc gatccacgcc agcaaggcgg caaactgaat
    18661 gaagtcacta aagaagacct gattaaactg gtcgagtttg ataacaaaga atatctctat
    18721 tacaaagcga ttgcgccaga tatcgccttc attcgcgcta ccacctgcga cagcgaaggc
    18781 tacgccactt ttgaagatga ggtgatgtat ctcgacgcat tggttattgc ccaggcggtg
    18841 cacaataacg gcggtattgt gatgatgcag gtgcagaaaa tggttaagaa agccacgctg
    18901 catcctaaat ctgtccgtat tccgggttat ctggtggata ttgtggtggt cgatccggat
    18961 caaacccaac tgtatggcgg tgcaccggtt aaccgcttta tttctggtga cttcaccctt
    19021 gatgacagta ccaaacttag cctgccccta aaccaacgta aattagttgc gcggcgcgca
    19081 ttattcgaaa tgcgtaaagg cgcggtgggg aatgtcggcg tcggtattgc tgacggcatt
    19141 ggcctggtcg cccgagaaga aggttgtgct gatgacttta ttctgacggt agaaacaggt
    19201 ccgattggcg gaattacttc acaggggatc gcctttggcg cgaacgtgaa tacccgtgcc
    19261 attctggata tgacgtccca gtttgatttt tatcacggtg gcggtctgga tgtttgttat
    19321 ttgagttttg ctgaagtcga ccagcacggt aacgtcggcg tgcataaatt caatggcaaa
    19381 atcatgggta ccggtggatt tattgatatc agtgccacct cgaagaaaat tattttctgc
    19441 ggcacgttaa ctgcgggcag tttaaaaaca gaaattaccg acggcaaatt aaatatcgtc
    19501 caggaaggac gggtgaagaa atttattcgg gaactaccgg aaattacttt cagcggaaaa
    19561 atcgctctcg agcgagggct ggatgttcgt tatatcactg agcgcgcagt attcacgctg
    19621 aaagaagacg gcctgcattt aatcgaaatc gcccctggcg tcgatttaca aaaagatatt
    19681 ctcgacaaaa tggatttcac cccagtgatt tcgccagaac tcaaactgat ggacgaaaga
    19741 ttatttatcg atgcggcgat gggttttgtc ctgcctgaag cggctcatta acaggagtat
    19801 aatgatggat ttttgtttaa ctgaagaaca agaactgctg ctggccagta ttcgcgaact
    19861 gattacgact aactttccgg aagagtattt ccgcacctgc gatcaaaacg ggacataccc
    19921 gcgtgaattt atgcgggcac tggcggataa cggtatttcc atgcttggcg tgccggaaga
    19981 atttggtggt atccctgcgg attacgtcac ccagatgctg gcgctgatgg aagtgtcaaa
    20041 atgcggtgct ccggcatttt tgatcaccaa cggtcaatgt attcacagta tgcgccgttt
    20101 cggttctgca gagcagctac gtaaaacggc agaaagcacc ctggaaacgg gtgatcccgc
    20161 ctatgccctg gcgttgacgg agccaggcgc aggctcagac aacaacagtg ccactaccac
    20221 ttacacgcgt aaaaacggca aggtttatat caacggacag aaaaccttta ttaccggtgc
    20281 gaaagagtac ccatatatgc tggtgttggc gcgcgatccg caaccgaaag atcccaaaaa
    20341 agccttcacc ctgtggtggg tcgactccag taagcccggc attaagatta acccgctgca
    20401 taaaatcggc tggcatatgc tcagcacctg cgaagtctat ctcgacaacg tggaagttga
    20461 agagagcgac atggtgggcg aagaaggaat gggtttcctc aatgtgatgt acaactttga
    20521 gatggagcgc ctgatcaacg ccgcgcgcag caccggcttt gccgagtgtg cctttgaaga
    20581 tgccgcccgc tatgccaacc aacgtatcgc ttttggtaaa cccattggtc ataaccagat
    20641 gatccaggaa aaactggcgc tgatggcgat taagatcgac aacatgcgca acatggtgct
    20701 gaaagtagca tggcaagccg atcagcatca gtcactgcgc accagcgcgg cgctggcaaa
    20761 actgtattgc gcacgtaccg caatggaagt cattgatgat gcgattcaaa tcatgggcgg
    20821 tctgggctat accgatgaag cgcgcgtctc ccgcttctgg cgtgatgtcc gttgtgaacg
    20881 tattggcggc ggtacagacg aaattatgat ttacgtagca ggtcggcaaa tcctgaaaga
    20941 ctatcagaac aaataatctg caggcggcgc agcttcttaa caaactgcgc cgccagattt
    21001 acccggcaag acttaccggt tgaggaaatt cagataagat ttcttgttcc gcaaaacagt
    21061 ttttaaattg cctgcggtaa tcgctgggcg aacaaccgac atggcgcaaa aacagtttgg
    21121 caaagtgatc gacattttca taacccacac gccaggagat ctctgcctgt gataattcag
    21181 tattagtgag taaccatttc gcttccgtca tacgccgttg aataacatag ttaatagggg
    21241 aaatacgata ctctttggta aattcatggc aaatataact gacgctggca cgaaattttt
    21301 tcgataa
//
LOCUS       DAZFFG010000084        21300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFFG010000084 DAZFFG010000000
VERSION     DAZFFG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.084"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHJ7J_003434"
     CDS             complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACHJ7J_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO5001620.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1574..2755)
                     /gene="hemX"
                     /locus_tag="ACHJ7J_003435"
     CDS             complement(1574..2755)
                     /gene="hemX"
                     /locus_tag="ACHJ7J_003435"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO5001621.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAETTEAKPAPAPQADTPAAAPQG
                     E"
     gene            complement(2777..3517)
                     /gene="hemD"
                     /locus_tag="ACHJ7J_003436"
     CDS             complement(2777..3517)
                     /gene="hemD"
                     /locus_tag="ACHJ7J_003436"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO5001622.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3514..4455)
                     /gene="hemC"
                     /locus_tag="ACHJ7J_003437"
     CDS             complement(3514..4455)
                     /gene="hemC"
                     /locus_tag="ACHJ7J_003437"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO5001623.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4842..7388
                     /gene="cyaA"
                     /locus_tag="ACHJ7J_003438"
     CDS             4842..7388
                     /gene="cyaA"
                     /locus_tag="ACHJ7J_003438"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO5001624.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTTDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7428..7748)
                     /gene="cyaY"
                     /locus_tag="ACHJ7J_003439"
     CDS             complement(7428..7748)
                     /gene="cyaY"
                     /locus_tag="ACHJ7J_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO5001625.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEKAATQQAGET
                     VSFR"
     gene            complement(7796..9121)
                     /locus_tag="ACHJ7J_003440"
     CDS             complement(7796..9121)
                     /locus_tag="ACHJ7J_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO5001626.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYGE
                     DEKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTPDHQWSKWIDLPDIEPEQFHLITGNIAQYKDWLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTRVREENVRELHNRDIIIPLTDESCRNAAAEVQTLKP"
     gene            complement(9118..10626)
                     /locus_tag="ACHJ7J_003441"
     CDS             complement(9118..10626)
                     /locus_tag="ACHJ7J_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO5001627.1"
                     /translation="MINTSRQDGFMHYRNVVCLLACSLFLSSAWGCRLDAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTQDEWNKEPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRNILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDDNGGGNSVDIKTLHQVEFRPPWDPDLLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLAWDEDLTKHCLDFSLLEKKVFWCQGPDCKQEELPGLDPEQ
                     FHPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(10963..11517)
                     /locus_tag="ACHJ7J_003442"
     CDS             complement(10963..11517)
                     /locus_tag="ACHJ7J_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO5001628.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKIYGTGKMN"
     gene            11867..12070
                     /gene="yifL"
                     /locus_tag="ACHJ7J_003443"
     CDS             11867..12070
                     /gene="yifL"
                     /locus_tag="ACHJ7J_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5001629.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12107..12931
                     /gene="dapF"
                     /locus_tag="ACHJ7J_003444"
     CDS             12107..12931
                     /gene="dapF"
                     /locus_tag="ACHJ7J_003444"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO5001630.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12928..13635
                     /gene="yigA"
                     /locus_tag="ACHJ7J_003445"
     CDS             12928..13635
                     /gene="yigA"
                     /locus_tag="ACHJ7J_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO5001631.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13632..14528
                     /gene="xerC"
                     /locus_tag="ACHJ7J_003446"
     CDS             13632..14528
                     /gene="xerC"
                     /locus_tag="ACHJ7J_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO5001632.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14528..15244
                     /gene="yigB"
                     /locus_tag="ACHJ7J_003447"
     CDS             14528..15244
                     /gene="yigB"
                     /locus_tag="ACHJ7J_003447"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO5001633.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15328..17490
                     /gene="uvrD"
                     /locus_tag="ACHJ7J_003448"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15328..17490
                     /gene="uvrD"
                     /locus_tag="ACHJ7J_003448"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO5001634.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVVNDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(17535..18437)
                     /locus_tag="ACHJ7J_003449"
     CDS             complement(17535..18437)
                     /locus_tag="ACHJ7J_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO5001635.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSEKKYEGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(18520..19284)
                     /gene="yigE"
                     /locus_tag="ACHJ7J_003450"
     CDS             complement(18520..19284)
                     /gene="yigE"
                     /locus_tag="ACHJ7J_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO5001636.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLANINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            19446..19502
                     /gene="ysgD"
                     /locus_tag="ACHJ7J_003451"
     CDS             19446..19502
                     /gene="ysgD"
                     /locus_tag="ACHJ7J_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO5001637.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            19654..20604
                     /gene="corA"
                     /locus_tag="ACHJ7J_003452"
     CDS             19654..20604
                     /gene="corA"
                     /locus_tag="ACHJ7J_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO5001638.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(20646..21137)
                     /locus_tag="ACHJ7J_003453"
     CDS             complement(20646..21137)
                     /locus_tag="ACHJ7J_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO5001639.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGSSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNLHDFKK
                     DNF"
     gene            complement(21134..>21300)
                     /locus_tag="ACHJ7J_003454"
     CDS             complement(21134..>21300)
                     /locus_tag="ACHJ7J_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO5001640.1"
                     /translation="AQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNF
                     NTIERIENYL"
BASE COUNT         4995 a         5488 c         5469 g         5348 t
ORIGIN      
        1 agctcaggtt aaatattcac tcaggaagtt attactcagg aagccaagag gattacagaa
       61 ttatctcata acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacga
      121 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      181 agagccgttt acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc
      241 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      301 ctccgtaata agagcaggcg tttttttatg tgtcaggaag gccccggagg tgcttgcctc
      361 cgggtgagaa ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga
      421 cgcatcgctg cagcttcttc tggtttgtgc agtctgtcca gcgcgtcggc aagccatgcg
      481 taatcgtagg cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc
      541 cattcgccgt gcttcatcag tgactgaccc agtgtgctcc acaacagcgg gcgatcgccg
      601 acgtttttga tttgctggcg cagcactttt tcaagctgtt cagggttatt ggttttcaga
      661 cgcgggatcg gcagcagtag gcgatcgtca tactggcgtt tcaggccgtc gatgataatt
      721 tgctgggcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac
      781 gctacctgat gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcgcta
      841 ccgttatcgg cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca
      901 cgatgttctt catcaccaac atgggctttc gccattgatg ggataatatc cagcagcgaa
      961 ctccatgcac ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1021 ggcgtaactt ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc
     1081 agttgcagac gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct
     1141 gcgcgttcca gatgttggtt ggcgcgcgct tcatcaccac gttgttgcgc ggcttcggca
     1201 gccagtagat agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt
     1261 tcaacttgct gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt
     1321 gcacggcgac gcttacgccc gacaaaccac ccacgggtgt gcgcgccagt gcggaagatc
     1381 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa tatgatcgcc
     1441 aggcccgtga cgctggtttc gatattgtag ttgtcggtct ggatcagcac ataaccctga
     1501 tgaccggcaa tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac
     1561 acttttagca tggttattct ccttgcggcg cggctgccgg agtatcagct tgcggtgcag
     1621 gtgcaggttt agcttccgtt gtctccgctg ccggttgtgc cagcaggtta cgcacgcgag
     1681 tctgcatcag tttttccagc atcgcctggc tttgcagggt ttccggaaga tccatcgaga
     1741 tattttgctg gcttaactgg tccacctcgt cgaggaacgc tttggtggtg gcatcatcag
     1801 tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga taagtctctt
     1861 cctgatgacg cggtacagct tgtgctgcga ccagcaggcg agagcgaata ttttcgcgca
     1921 gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga cggcgaatcg
     1981 taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc cattcgctga
     2041 tggaactgga aagctcttca ccgtcggaat ccatcggcga accatcgctg tcattatcgg
     2101 ccagacgcag gttatctacc tgatttgaaa gctgattaag cttaaggatg atgccgtcat
     2161 aatccacctg cgatactgca gaaaggctgg cgatatcatc ggtaattgcc cgacgaacgg
     2221 taatcaaact cgggtcattc atatccgcca ggctggcgtc tgcacttttc agcaacgctg
     2281 cagcggtcgt gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc accagaaaat
     2341 cggcctgagc cagcagccag gttttagcat cgctgccgga aatggtggcg accttttgtt
     2401 ggacttcatc caactgtttt gccagcgttt cttgctgacg attcgcctgc ttaagttgtg
     2461 cagcttgttg cttaataatg ccttccagct cggctttttg gctctcctgg gctttttgca
     2521 atgccgtcag ttggttagcc agggcatcgc tggtggcggt ctgattgacg gcctgttgtt
     2581 taccccagcc atacaaaccg acgcctgccg ccagagcaat agcgatagcc accgcactga
     2641 gaatcaatgc ggtattgttc ttactctttt tttctgttgc gacaggttgt gacgtggtgt
     2701 ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc gtcattatgg
     2761 cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca gcgttatcgg
     2821 cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc tcactgacga
     2881 ccaatagtcg acagtgtagt aaccagtgct cacgatacca ttgtgggatc agcgaccaga
     2941 gttgctgcaa catttcacca ctggtaacaa cgaccgtcgt cacctcgcgg gattgccagc
     3001 gcatcgcttc ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat tcacaaaaag
     3061 tgacctcagc accgcgcgcc gtcagggtat ccccaattag ctcacgaccg ccattgccac
     3121 gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt agcaagactt
     3181 cgctgatttc ccgatcctgt gggtagagaa tcttctgtcc gcttacggta tgtagcgcca
     3241 gtgcggtggt gcgtccaatg gcgaaataag taggtagtcg gggccattta cgatcttgct
     3301 gatgcagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac aacagatcgc
     3361 tctcccccag cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc ggagaaaact
     3421 caatcagcgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc actaactctt
     3481 ctccagcggg agacgggcgg gtgacaagga tactcatgcc ggggcgtctc cgttatagac
     3541 ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg aaatccccat
     3601 ttgttcggca ttttgcggcg caccgcggcg ttcaccgcga ataatctgcg aaccgtccgg
     3661 cgcgccgacc agcgcacgca gccagatttc gccatcaata agctcggcgt agctaccaat
     3721 tggcacctga catccgcctt cgagacgggt attcatggcg cgttctgcgg taacgcgcag
     3781 tgcagtttcg tggtgattca gcgcggcaag cagctcgcga gtgcgtgtat catcaaggcg
     3841 gcattcaata ccgactgcac cttgtcctac cgccggaaga gaaatctcgg gtggcaacgc
     3901 ggcgcgaatg cgggactcca gacctaaacg ttttagtccg gctacggcaa gaatgatggc
     3961 atcgtattcg ccgttatcca gtttgctcag gcgagtgccg acgttgccgc gcagggagcg
     4021 gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac tggacgtccc
     4081 gacgatactg cctgccggta acgcatccag actgtcatag ttattggaca caaaggcatc
     4141 gcgaggatct tcacgctcac aaatagtgac cagccccaga ccttgcggga attcaaccgg
     4201 cacatctttc attgagtgta cggcgatatc ggcgcgattt tcgaggagcg cgacttccag
     4261 ctcttttaca aataagcctt ttccgccgac tttcgccagc ggcgtatcaa gaatcacatc
     4321 gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg ccatcaactt
     4381 gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg caattcttaa
     4441 aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct aacatccttg
     4501 ccagagtgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt aaaatgaggt
     4561 ctggcagtgg atcctgacag gcgtttcacg ccgtcgtaat aaggaattta cagagaataa
     4621 acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa ggtgttaaat
     4681 tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg cgaaaagtgg
     4741 taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt tgatgttggc
     4801 ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc tatattgaga
     4861 ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg cttgctgcta
     4921 tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac tatcaccatc
     4981 cgctaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt tacacgactg
     5041 atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg tcagtacagg
     5101 acccgccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc acctcgtccg
     5161 tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc tggctcgata
     5221 gcgaagagcg ccagttgcta caacgtaaat gcagcctgct ggaaagctgg gccgcctcgc
     5281 tgggtgtgga agtcagcttc ttcctgattg atgaaaaccg cttccgtcat aatgaaagcg
     5341 gcagcctggg gggcgaagat tgtggctcca cccagcatat actgctgctt gacgaatttt
     5401 atcgtaccgc cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg ccgtgcgacg
     5461 aagaagagca ttacgacgac tatgtgatga cgctttacgc gcagggcgtg ctgacgccaa
     5521 atgaatggct ggatctcggc ggcttaagct cgctttccgc tgaagaatac ttcggtgcca
     5581 gcctttggca actctacaag agtatcgatt ctccatacaa agcggtgctg aagacactgc
     5641 tgctggaagc ctattcctgg gaatacccga acccacgtct gctggcgaaa gatatcaaac
     5701 agcgtttgca cgacggcgag attgtatcgt ttggtctcga tccatactgc atgatgctgg
     5761 agcgtgttac tgaatacctg acggcgattg aagatttcac ccgtctggat ttagtacgtc
     5821 gctgcttcta tttaaaagtg tgcgaaaagc tcagccgtga acgcgcctgc gtaggctggc
     5881 gtcgcgcagt gttgagccag ttagtgagcg agtgggggtg ggacgaagct cgtctggcaa
     5941 tgctcgataa ccgcgctaac tggaagattg atcaggtgcg tgaggcgcac aacgagttgc
     6001 tcgacgcgat gatgcagagc taccgtaatc tgatccgctt tgcgcgtcgc aataacctta
     6061 gcgtctccgc cagtccgcag gatatcggcg tgctgacgcg taagctgtat gccgcgtttg
     6121 aagcattacc aggtaaagtg acgctggtaa acccgcagat ttcacccgat ctctcggaac
     6181 cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt tggtatctgt
     6241 ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa tataaccgtt
     6301 acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctcg cgcacccgct
     6361 tgtatattaa aggtaacggc attgtcgatt tgcctaagtt gcaggagatg gtcgccgacg
     6421 tgtcgcacca tttcccgctg cgcttacctg caccgacacc gaaggcgctc tacagcccgt
     6481 gtgagatccg ccatctggcg attatcgtta acctggaata tgacccgaca gctgcgtttc
     6541 gcaatcaggt ggtgcatttt gatttccgta agctggatgt cttcagcttt ggcgagaatc
     6601 aaaattgcct ggtaggtagc gttgacctgc tgtatcgcaa ctcgtggaat gaagtgcgta
     6661 cgctgcactt caacggcgag caatcgatga tcgaagccct gaaaactatt ctcggcaaaa
     6721 tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgttatagc cagcatctgc
     6781 gcggcttaat tcgtactcgc gtgcagcaac tggtttctga gtgtattgaa ttgcgtcttt
     6841 ccagcacccg ccaggaaacc gggcgtttca aggcgctgcg cgtttctggt caaacctggg
     6901 gcttgttctt cgaacgcctg aatgtatcgg tacagaaact ggagaacgcc atcgagtttt
     6961 atggcgctat ttcgcataac aaactgcacg gcctgtcagt gcaggttgaa accaatcacg
     7021 tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag ttctttttcg
     7081 aagaaacgca agatgagaat ggctttaata tctacattct cgacgaaagc aaccgggttg
     7141 aggtgtatca ccactgcgaa ggcagcaaag aggagctggt acgtgacgtc agtcgcttct
     7201 actcgtcatc gcatgaccgt tttacctacg gctcaagctt catcaacttc aacctgccgc
     7261 agttctatca gattgtgaag gttgatggtc gtgaacaggt gattccgttc cgcacaaaat
     7321 ctatcggtaa catgccgcct gccaatcagg atcacgatac gccgctatta cagcagtatt
     7381 tttcgtgatg aacgtgccgg aaagcgaggc ttatccggca tgcaatctta gcggaaactg
     7441 actgtttcac ccgcctgctg cgtcgccgcc ttttccagca aatcccagaa ggtttcgccg
     7501 ctgcgatcgc aaatccactc atcgcctttc agatcaaaat ggtagccgcc ttgtttggtt
     7561 gccagccata cctggtgcag cggttcctgg cggttgataa tgattttgct gccgttttca
     7621 aaggtaatgg tcagtacacc gccgttgatt tcgcagtcga tatcgctgtc gccatcccag
     7681 tcgtccaggc gttcttcaat ggtcagccag agttgatcag ccaggcgatg aaattcactg
     7741 tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca tggggtcagg
     7801 gcttcaaagt ttgcacctct gcggctgcgt tccggcacga ttcatccgtc aacggaataa
     7861 taatgtctct gttgtgaagt tcacgcacat tctcttctct gacgcgtgtt ttcgccgggc
     7921 agggggaggg cgtaatcctt acctctgtat cggtccatgt ataaacattg cgatcgtcat
     7981 aagcgaagcg atcgttttta gttagcggac cgttaactgg aataatttgt acgctctttg
     8041 accgtaattg gcggataaaa taagcatgct gtttgccgct attaaatgag cgatcaatga
     8101 ccaggtctgg cgtatccagc gggattatct caagctggtc ttcaccaaat gtcgataatt
     8161 ttgttacata caaccaatct ttatattgcg caatattgcc agtaatcaga tgaaattgtt
     8221 caggttcaat atctggtagg tctatccact tgctccactg atggtcgggc gtgagctgtt
     8281 tacgccagcg aacgcgactt tcccctactt caaaagagac gtcatcatct acgggtatcg
     8341 ctttgcctga taattgacca aagggataac gatgcagacc cttattgtcg cgaaaaacca
     8401 gcagtgaatg aggtatccag cgaattatct caaatgagtc tgcattcgcc gtaaggtgaa
     8461 cgccatttaa ataaatattc tgcccactgc ggataagcag atcattggaa tacgaatttc
     8521 tgtttacact cataagacgt ggattaatac cccaccatct tttttctaat acagtaacat
     8581 cactgctgct cccctgacgt cgaccggaca aatagagatt tttactatcc accagcgtgg
     8641 tagagttacc ttcgatagct tttagtgtcg ccagatcaac acgtgacgcg ccgctattac
     8701 tttcggtgcg ctgtccgtca aaatagaggc tatatttatc gaccgcgaag cggcccatag
     8761 cctgaaagct ggcaatatcg acagtgggtg ttcccggtgg attgctaacg attttgccac
     8821 gccagagaat aacgcggccg tctgtcaccc agtggtattc ctcatttttt atgcttattt
     8881 catcagtggg tatatagcct tccactaact cccgctcccc ggatatattt gtcacaaggc
     8941 gcaaatcggc tctgttcatc ttcggaaatt gggcaatgat tttctcatcc tccccataac
     9001 ctttaccgta agacataaca ccgtgatcat tgtaatagtc gaaccctatg gcctgtacag
     9061 caggtgtcag aaatgtgtgc cccaacagga tgagtgcaac tgtgattatt tgacccttca
     9121 ttttgtcgcc tcattgcgcg gggcaggaat aaattcgccg tcatcggtaa tttttccgtc
     9181 cagatatccc gttttgtaat ttaccccgta gccatatccc gaatgttgtt ccttccagat
     9241 taagcgaact tgtttcggat cagcggtgcg gtatcgccag agctgcgcgc catcaaagct
     9301 gtaaacgtag cggttatcgc gggcatacca tctgcgcaaa aagggatggt cattaagatg
     9361 ggcttcgcgt tcatccggag aacggttatc cagtaagatc agcggtccgt cagattcaaa
     9421 cacgtgtaaa ccggaatggt atggccaccc ttcggcacgc gtaagtaaat agccgtggcg
     9481 tttcccggcg ttgaaacgtt tgttgataat aagattggga tcatcaagtt tcacgatctc
     9541 cagcttgcgg tcaccagact ctgtttcgat gatggtataa agcgagtcct gatactgtgc
     9601 gacagcatca ctgatggggt gaaactgttc cggatcgagt cccggtaatt cttcctgttt
     9661 acagtcaggc ccctgacacc agaacacttt cttttccagc agagaaaaat ccagacagtg
     9721 ctttgttaaa tcctcatccc acgccagatt ttccttgtcg aggactttac gctgtagccc
     9781 gtttttatca cgccaggtca agagtgagcc gggcatccag cgcacgacgg aaaaggtgtc
     9841 cgggtcggca tcaagctgtt cgccgttgat caggattttg gttcttgtac gagccagggt
     9901 atcccagggg ccaaatggca tagccacaca gggattaaac gcggtagaaa actttcctct
     9961 ttgatcccat gacttttgtg ccagcacacg gaaactctcg gggtcagcga ggcgatgccc
    10021 atcgacatat aagaaattag cgtcacgtaa tatcagcccc agtaagtcag gatcccaggg
    10081 aggccggaat tccacctgat gcagtgtttt gatatctacg ctatttcccc cgccattgtc
    10141 atcggtgcgc ttgccttcga aatagagact gtgtttatct gcggcaaaat caccccatgc
    10201 cttgaacgag gcgacatcga cgggaggttt gtctggcggg ttttgcacaa cttcacccgc
    10261 gtagaggata ttgcggccat cgctatgcca ggagtaatga ggataccagg cttgtgcgcc
    10321 ttcatagagg tagtcggttg ttagatcagt cagaggtggt tctttattcc attcatcttg
    10381 tgttgttggg tctattaata aattgggtaa acggcgcaaa ctcttgtaat tcacgtcagg
    10441 tagagaaaga tttgtttctt tataagggcg caggtacacc acgccttttc cctgtttctg
    10501 gtaaatatta tgttcaggtg catcaagccg acatccccat gccgacgata agaacaatga
    10561 acaagccaac aaacaaacca cattgcgata gtgcataaag ccatcctggc gcgaggtgtt
    10621 gatcacgata ctgccagcag aacataaatc ctcacacgta agcgtaactc tcgcggcaaa
    10681 tcgtcacttt tcagaatcca taaaggtcat tgctattttc tgatagttgc tggggtaaac
    10741 gtgatgtttt cattcacgat ataaatacga ggtactgcgc catggtgtga ctgatcacgt
    10801 taaaatgtgc cgggttgacg ccgggaaggg tttcaacatg cgactctgcc cccgttgcct
    10861 actttattgt ctttcgcgag ttgtacccat gttccaccac tggtatctaa cattctccac
    10921 attattacgc tttctgccga atgtgatttt tatagaaatc tatcaattca ttttccctgt
    10981 gccataaatt ttatccttca catcctgccg aacgcgggtc ttgtaccccc cgttgtgaag
    11041 atcttcatac ggagaaaaag aatctttact ccaccccagc agcactttat cgccaggcat
    11101 aaaatgcaca tgcagaaaat cttctcccca ttctcttttg gggagaggta acgtcatatt
    11161 ccgtttctcc agacgcattc cagcattata ctgtgtacga gtagtactta atgtccagat
    11221 aacctctgcc gtatccccac tgacatcacc acaacaggtt gttgaacctc caccatttgc
    11281 aaaagcattc gcgcccgcca cgccgttaac actgaatccc agtatcggac ggtcaatttc
    11341 actgtggatt tccagaatca ctccacctct tggacctccc catactgcct gccctatatt
    11401 ccacaacacg accagtactg gaacgacaat taacgtccgc catacccagc gcccccagcg
    11461 ggcataaccg cgcgtaagct tttggtcaaa tttttccagt tgggtaaata gccccatcag
    11521 attttattcc ctcttatcac caggttggca aagttgtatg tcactggcga ctaaggtcag
    11581 acaacgaaat tgcatggtgc gccactctat taaaagcccc atgccgacga taaaaacaac
    11641 gaacaagaca acaaataaac cacattgcga tagtgcataa agccatcctg gcgcgaggtg
    11701 acgatcacga aactaccagc aaaacataaa tcctcacgag taagcgttat actcgcagca
    11761 tttcctcact tttcagactt cataaagagt cgctaaatgc ttgcttttac gccttctcct
    11821 gcgatgatag aaagcagaaa gcgatgaact ttacaggcaa tccataatga aaaacgtgtt
    11881 taaggcactc actgtattac ttactctctt cagcctgacg ggctgcggtc tgaaaggtcc
    11941 gctctatttc ccgcctgcag ataaaaatgc accgccgccg accaaaccgg tagagacgca
    12001 aacgcaatcc acggtgccgg ataaaaacga tcgcgccact ggcgatggtc catcccaggt
    12061 gaattactaa aagtcagttt ctgtacccgc gtgattggag taaatgatgc agttctcgaa
    12121 aatgcatggc cttggcaacg attttatggt cgtcgacgcg gtaacgcaga atgtcttttt
    12181 ttcaccggag ctgattcgtc gcctggcgga tcggcacctg ggggtagggt ttgaccagct
    12241 gctggtggtt gagccgccgt atgatcctga actggatttt cactatcgca ttttcaatgc
    12301 tgatggcagt gaagtggcgc agtgcggcaa cggtgcgcgc tgctttgccc gttttgtgcg
    12361 cctgaaaggg ctgaccaata agcgcgatat ccgcgtcagc accgccaacg ggcggatggt
    12421 tctgaccgtc accgatgatg atctggtccg cgtaaatatg ggcgaaccca acttcgaacc
    12481 ttccgctgtg ccgtttcgcg ctaacaaagc ggaaaagacc tatattatgc gcgccgccga
    12541 gcagacaatc ttatgcggcg tggtgtcgat gggaaatccg cattgcgtga ttcaggtcga
    12601 tgatgtcgat accgcggcgg tagaaacgct tggtcctgtt ttggaaagcc acgagcgttt
    12661 tccggagcga gccaatatcg gttttatgca agtggttaag cgcgagcata ttcgtttacg
    12721 cgtttatgag cgtggggcag gagaaaccca ggcctgcggc agcggcgcgt gtgcggcggt
    12781 tgcagtaggg attcagcaag gtttgctggc cgaagaagta cgcgtggaac tccccggcgg
    12841 tcgtcttgat atcgcctgga aaggtccggg tcacccgtta tatatgactg gcccggcggt
    12901 acatgtctac gacggattta ttcatctatg aagcaaccag gggaagaact gcaggaaaca
    12961 ctcacggagc ttgatgaccg ggcggttgtc gattatctga ttaaaaatcc tgagtttttt
    13021 atccgcaatg cgcgcgcagt agaagcgata cgtgtgccgc atccggtacg cggcaccgtt
    13081 tcgttggtcg agtggcacat ggcccgcgca cgtaatcata ttcatgttct ggaagagaac
    13141 atggcgctgt tgatggaaca ggctatcgcc aacgaaggcc tgttttatcg cctactctac
    13201 ctgcagcgca gtctcaccgc cgccagcagt ctcgacgata tgctgatgcg ctttcaccgc
    13261 tgggcgcgcg atctcggcct ggcaggtgcg agtctgcgcc tgtttccgga tcgctggcgc
    13321 ttaggtgcgc cgtcgaacca cactcatctg gcattaagcc gtcagtcttt cgaaccgctg
    13381 cgtattcagc gtttggggca ggaacagcac tatcttgggc cgcttaacgg accagagctg
    13441 ctggtggtgc taccggaagc gaaagcggtg ggatcggtgg cgatgtcgat gctgggaagc
    13501 gatgctgatt tgggtgtcgt gctgtttacc agtcgcgatg ccagtcacta tcaacaaggg
    13561 cagggaacgc agttacttca tgaaattgcg ctgatgttgc cggagcttct ggagcgttgg
    13621 attgaacgcg tatgaccgat ttacacaccg atgtagaacg ctacctacgt tatctgagcg
    13681 tggagcgtca gcttagcccg ataaccctac ttaactacca gcgtcagctt gaggcgatca
    13741 tcaattttgc cagcgaaaac ggcctgcaaa gctggcaaca atgcgatgca gcgatggtac
    13801 gcaattttgc tgtacgcagt cgccgtaaag ggctgggagc agcaagtctg gcgttacggc
    13861 tttctgcgct acgtagcttt tttgactggc tggtcagcca gaacgaactc aaagctaacc
    13921 cggcgaaagg agtttcggca ccgaaagcgc cgcgtcatct gccgaaaaac atcgacgtcg
    13981 acgatataaa tcggctgctg gatattgata tcaacgatcc cctcgctgta cgcgaccgtg
    14041 caatgctgga agtgatgtac ggcgcgggtc tgcgtctttc cgagctggtg gggctggata
    14101 tcaaacacct cgacctggag tctggtgaag tgtgggtaat ggggaaaggc agcaaagagc
    14161 gccgcctgcc gattggtcgc aacgctgtgg cgtggattga gcactggctt gatttgcgcg
    14221 acctgtttgg tagcgaagac gacgcgcttt ttctgtcgaa actgggcaag cgtatctccg
    14281 cgcgtaatgt gcagaaacgc tttgccgaat ggggcataaa acaagggctg aataatcacg
    14341 ttcacccgca taaattacgt cactcgttcg ctacgcatat gcttgagtcg agcggcgatc
    14401 ttcgtggtgt gcaggagctg ctgggtcatg ccaacctctc caccacgcaa atctatactc
    14461 atcttgattt tcaacacctt gcctcggtgt acgatgcggc gcatccacgc gccaaacggg
    14521 ggaaataatg cgtttttacc ggcctttggg gcgcatctcg gcgctcacct ttgacctgga
    14581 tgataccctt tacgataacc gtccggtgat tttgcgcacc gagcgagagg cgcttacctt
    14641 tgtgcaaaat tatcatccgg cgctgcgcag cttccagaat gaagatctgc aacgcctgcg
    14701 ccaggcggta cgggaagcgg aacccgagat ttatcacgac gtgacgcgtt ggcgttttcg
    14761 ttcgattgaa caagcgatgc tcgacgccgg gctgagtgcc gaagaagcca gtgcaggcgc
    14821 acacgcagca atgattaact ttgccaaatg gcgcagccgg atcgacgtcc cgcagcaaac
    14881 ccacgacacc ttaaaacagc tggcgaagaa atggccgctg gtggcgatca ccaacggtaa
    14941 cgcccagcca gaactgttcg gcctgggcga ctattttgag tttgtgctgc gcgctggccc
    15001 gcacgggcgc tcaaaaccgt tcagcgatat gtacttcctt gcggcggaaa aactcaacgt
    15061 gccgatcggc gagatcttac atgtcgggga cgatctcacc actgacgtgg gtggggcaat
    15121 tcgcagcgga atgcaggcct gttggatcag accggaaaat ggcgatctga tgcaaacctg
    15181 ggacagccgt ttactcccgc atctggaaat ttcccggttg gcatctctga cctcgctgat
    15241 ataatcagca aatctgtata tatacccagc tttttggcgg agggcgttgc gcttctccgc
    15301 ccaacctatt tttacgcggc ggtgccaatg gacgtttctt acctgctcga cagccttaat
    15361 gacaaacagc gcgaagcggt ggccgcgcca cgcagcaacc ttctggtgct ggcgggcgcg
    15421 ggcagtggta agacgcgcgt actggtgcat cgtatcgcct ggctgatgag cgtggaaaac
    15481 tgctcgccat actcgattat ggcggtgacg tttaccaaca aagcggcggc ggaaatgcgt
    15541 catcgtatcg ggcaactgat gggcaccagc cagggcggca tgtgggtagg caccttccac
    15601 gggctggcgc accgcctgct gcgtgcgcac catatggacg ccaatctgcc gcaggatttc
    15661 cagatcctcg atagcgagga ccagctgcgc ctgcttaagc gtctgatcaa agccatgaac
    15721 ctcgacgaga agcagtggcc gccgcgccag gcaatgtggt acatcaacag ccagaaagat
    15781 gaaggcctgc gtccgcatca tattcaaagc tacggtaatc cggtggagca gacctggcag
    15841 aaggtgtatc aggcgtatca ggaagcgtgc gatcgtgcgg gtctggtgga cttcgccgag
    15901 ctgctgctgc gcgctcacga gttgtggctt aacaagccgc atatcctgca acactaccgc
    15961 gaacgtttta ccaatatcct ggtggacgaa ttccaggata ccaacaacat tcagtacgcg
    16021 tggatccgcc tgctggcggg cgacaccggc aaagtgatga tcgtcggtga tgacgaccag
    16081 tcaatctacg gctggcgcgg ggcgcaggtg gagaatattc agcgtttcct taatgatttc
    16141 cccggtgccg aaactattcg tctggagcaa aactaccgct ctaccagcaa tattctgagc
    16201 gccgcgaacg ccctgattga aaacaacaac gggcgtctgg gtaaaaaact gtggaccgat
    16261 ggcgcggacg gtgagccgat ttccctctat tgcgctttta acgaactcga cgaagcgcgt
    16321 tttgtggtta atcgcatcaa aacctggcag gacaacggcg gggcgcttgc cgagtgcgcc
    16381 attctctacc gcagcaacgc ccagtcgcgt gtactggaag aggccttatt acaggcgagt
    16441 atgccgtacc gtatttacgg cgggatgcgc ttcttcgaac gccaggaaat caaagatgcg
    16501 ctctcgtatc tgcgcctgat tgccaaccgc aacgacgacg cggcctttga gcgcgtagtg
    16561 aatacaccaa cgcggggtat tggtgaccgg acgctggacg tggtacgtca gacatcgcgc
    16621 gatcgccagt taacactctg gcaggcatgt cgtgaactgt tgcaggaaaa agccctcgcc
    16681 ggacgtgctg ccagcgcctt acagcggttt atggaactga tcgacgcctt agcgcaggaa
    16741 actgccgata tgccgctgca tgtacagact gaccgggtaa ttaaagactc cggcctgcgt
    16801 accatgtacg agcaggagaa gggcgaaaaa ggtcagacgc gtatcgaaaa cttagaggaa
    16861 ctggtgacgg caacgcgcca gttcagctac aacgaagaag acgaagattt aatgccgctg
    16921 caggcgttcc tctcccatgc ggcactggaa gcgggcgagg ggcaggcgga tacctggcag
    16981 gacgcggtgc agttgatgac gctacactcg gcgaaagggc tggagtttcc gcaggtgttt
    17041 atcgtcggta tggaagaggg catgttccca agccagatgt cgctggatga aggcggtcgt
    17101 ctggaagaag aacgccgtct ggcctacgtt ggcgtaaccc gtgcgatgca gaaactgacg
    17161 ctgacctacg cggaaactcg ccgtctgtat ggcaaagagg tttaccatcg cccgtcgcgc
    17221 tttatcggtg agttgccgga agagtgtgtg gaagaggtgc gcctgcgcgc cacggtaagc
    17281 cgcccggtca gccatcagcg tatgggtacg ccgatggtcg tgaacgatag cggctacaaa
    17341 cttggccagc gcgtgcgcca cgccaagttt ggtgaaggca ctatcgtcaa tatggaaggc
    17401 agcggcgagc atagccgttt gcaggtggca ttccagggcc agggtattaa atggctggta
    17461 gcggcttacg cccggctgga gacggtataa cgttgcggga tgcggtgcgc cacaccgcat
    17521 ccggcatcag caccttactt agcgcacttc ttcacatcaa tattccagtc agcaccagac
    17581 gcaacctgat gtttctgcgc gaaattatcc atattcagcg ccacggaaac attcaataag
    17641 ctgttcagat acaccatcgg ctgtccttcc ggcacatcgc cgaagctggc gacatacggc
    17701 attttccctt cgtatttttt ctcggaacct tcggaaatgg tcacacacaa cgtgtcgttg
    17761 agtttgatcc ccgcctgatt cagcaactcg tcgctgatat tgctccagac gttgccgtac
    17821 tgaatatcga gaatcgggat attgcccttc acttcacctt ttgttgcttt ggctttctga
    17881 taagaaagtt cgacaacttt tgcaggaagc tctggcccca cctgctcgaa cgtgatcgct
    17941 ccggaagcca gacgcgcacc ggtgtaggca tatacatcgc gaccatggaa ggtatatgac
    18001 ttttcggaac ctttcagacg gttggttttt tcatcgattt gccggacgct ctcaataccc
    18061 agcgattccg ccaccaaagt gagcgtaccg ttatccggtg aaacgaagta ttgaccgttt
    18121 ttggttttca gcactaccga tttacggtct gtaccaacac ctggatcgac cacactgaca
    18181 aataccgaac cttgcggcca gtaactggcg gtctgataca ggcggtaagc gccttcccag
    18241 atgttatacg gtggaatttc gtgtgttaaa tcaaatattt taagattatg gtcaacaccg
    18301 aatgcgaccc ctttcatcgc cgagacggca ccatctttca ggctaaaatc agtttgtaaa
    18361 atcaaagcgt tatccgcata agcggatgat gatcccagac acgttaataa aaataacgtt
    18421 gcgagcgttt ttttcatttt tgtttccctt catttatctg atgattttcc agctgtgtag
    18481 ccagccccag tttgtctggc ctcctcgagt gtacaatgct taacctttcc gctcgacgga
    18541 aatcatggtg acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg
    18601 tgaaatcgtg ccatcaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca
    18661 ggcaaaatca taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt
    18721 attaattcca acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt
    18781 aattacactg ttttccatca acattggccc tgactgcacc gcaaactgaa tctctttact
    18841 ggttttgaag gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc
    18901 gccaggacgg ataaagaaat tcccttcacc tgaagcgaga tttaacgcca ccttctgctg
    18961 accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc cgccgttcat
    19021 cgccatctgc acctgaccct gactattaat attcgccagc agagcatgta acgttcccca
    19081 cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag gattaacggt
    19141 atacgcctgt acggtcagcg ttggatctga gagtgcgcaa tcatcagcgg caacagcaaa
    19201 caagggaagt aaggtgagag tgagaaaaat ccgtttgaga ttcaaggtga tcattccttt
    19261 accaatgagt agccgatgcg ccattatagg tcctggatgt gggatttttt tatcctgtta
    19321 gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg tgttctgttg
    19381 cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct aagaggacat
    19441 tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg atcaactcct
    19501 aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat ttttcccgtc
    19561 gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg cccaccgaac
    19621 tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca actggaaaat
    19681 aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc agtatggatt
    19741 gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact tggccagagc
    19801 ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cccgtttctt tgaagacgat
    19861 gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca cgccggtaac
    19921 tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga gcgtgaactg
    19981 cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga cggtaacgcc
    20041 tacgagctgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga tgaaattgaa
    20101 aatatctata gcgacctgga gcaattgagc cgggtgatta tggaagggca tcagggcgat
    20161 gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg gaaagtgcgc
    20221 ctgtgtctga tggataccca gcgcgcgctc aacttcctgg tacgtaaagc acgcttaccg
    20281 ggtgggcaac tggagcaggc gcgtgaaatc ctgcgagata tcgaatccct gctgccgcat
    20341 aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg ttttatcaac
    20401 atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt cctgccgcca
    20461 acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact gaagtggagt
    20521 ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc gtatctgtac
    20581 tttaagcgga agaactggtt gtaatatgag tgccggataa agctattttt ttatccggct
    20641 tatttctaaa aattgtcttt tttaaagtca tgcagattga aaactcgctc tattttattt
    20701 tcttgtgtta attgatactc agtaaaatcg ttaataccaa tatgagtgtt acgataaggg
    20761 tattcattaa taaccattaa gtaatttttc acgatgcaat tatcattgct cttaattgtc
    20821 gggaaacagt agagatcttc atacggccct tcgatatatt ttttatagtc agtgaagcca
    20881 ttaccgatat caatgacata ccaggtatgc caactacctt ctggtagggg atgttgattt
    20941 ttaatttcat tctgatgctt cagataccaa tttattttat cgctgtcagt ccatggcaga
    21001 tgatcaacga ccagaaagac tgaactgcca gcatcagagt atgtatacaa aatctgtaca
    21061 ggacgcaatg tccataaatt atagatgcag catagaagaa ataaacataa tataattttc
    21121 tggagtttgc ttttcataga taattttcga ttctttcgat agtgttaaaa ttggtgaaaa
    21181 aaggtttgaa agcaaagtct ttatgccgtt gtaaaaagaa ccatatgcga aaaaatctat
    21241 atttgctgta agtcttgttt ttgatatcag ttacatcaag accgaagtgg tcctgtgcta
//
LOCUS       DAZFFG010000085        21038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFFG010000085 DAZFFG010000000
VERSION     DAZFFG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.085"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..797
                     /gene="gadX"
                     /locus_tag="ACHJ7J_003455"
     CDS             <1..797
                     /gene="gadX"
                     /locus_tag="ACHJ7J_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO5001641.1"
                     /translation="AYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLV
                     SRDTLSLFLPMLKEEALNIHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEML
                     RKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLM
                     SPSLLKKKLREEETSYSQLLTECRMKRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVF
                     RNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGADCSAEGIRL"
     gene            1165..1893
                     /gene="gadW"
                     /locus_tag="ACHJ7J_003456"
     CDS             1165..1893
                     /gene="gadW"
                     /locus_tag="ACHJ7J_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO5001642.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2256..5369)
                     /gene="mdtF"
                     /locus_tag="ACHJ7J_003457"
     CDS             complement(2256..5369)
                     /gene="mdtF"
                     /locus_tag="ACHJ7J_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO5001643.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALLARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5394..6503)
                     /gene="mdtE"
                     /locus_tag="ACHJ7J_003458"
     CDS             complement(5394..6503)
                     /gene="mdtE"
                     /locus_tag="ACHJ7J_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO5001644.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLNELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQI
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6611..6889
                     /locus_tag="ACHJ7J_003459"
     CDS             6611..6889
                     /locus_tag="ACHJ7J_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001645.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6890..7417)
                     /gene="gadE"
                     /locus_tag="ACHJ7J_003460"
     CDS             complement(6890..7417)
                     /gene="gadE"
                     /locus_tag="ACHJ7J_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5001646.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8216..8788)
                     /gene="hdeD"
                     /locus_tag="ACHJ7J_003461"
     CDS             complement(8216..8788)
                     /gene="hdeD"
                     /locus_tag="ACHJ7J_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO5001647.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9043..9375
                     /gene="hdeA"
                     /locus_tag="ACHJ7J_003462"
     CDS             9043..9375
                     /gene="hdeA"
                     /locus_tag="ACHJ7J_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO5001648.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPINSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9491..9817
                     /gene="hdeB"
                     /locus_tag="ACHJ7J_003463"
     CDS             9491..9817
                     /gene="hdeB"
                     /locus_tag="ACHJ7J_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO5001649.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            9881..10528
                     /gene="yhiD"
                     /locus_tag="ACHJ7J_003464"
     CDS             9881..10528
                     /gene="yhiD"
                     /locus_tag="ACHJ7J_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO5001650.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10580..11350)
                     /locus_tag="ACHJ7J_003465"
     CDS             complement(10580..11350)
                     /locus_tag="ACHJ7J_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO5001651.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLAQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(11347..12339)
                     /gene="shuU"
                     /locus_tag="ACHJ7J_003466"
                     /gene_synonym="chuU"
     CDS             complement(11347..12339)
                     /gene="shuU"
                     /locus_tag="ACHJ7J_003466"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO5001652.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(12390..13013)
                     /gene="chuY"
                     /locus_tag="ACHJ7J_003467"
     CDS             complement(12390..13013)
                     /gene="chuY"
                     /locus_tag="ACHJ7J_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO5001653.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSDRAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVRRADVAAHIHELANAPALNKQIYSLIEPDLKPA"
     gene            complement(13013..13507)
                     /gene="hutX"
                     /locus_tag="ACHJ7J_003468"
     CDS             complement(13013..13507)
                     /gene="hutX"
                     /locus_tag="ACHJ7J_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO5001654.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVKNLPSSTIVSG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(13520..14857)
                     /gene="hutW"
                     /locus_tag="ACHJ7J_003469"
     CDS             complement(13520..14857)
                     /gene="hutW"
                     /locus_tag="ACHJ7J_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO5001655.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDGCLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            complement(14877..15791)
                     /gene="shuT"
                     /locus_tag="ACHJ7J_003470"
     CDS             complement(14877..15791)
                     /gene="shuT"
                     /locus_tag="ACHJ7J_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO5001656.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQFP
                     EQGEALVTQISQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            16473..18455
                     /gene="chuA"
                     /locus_tag="ACHJ7J_003471"
                     /gene_synonym="shuA"
     CDS             16473..18455
                     /gene="chuA"
                     /locus_tag="ACHJ7J_003471"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO5001657.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     IDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            18504..19532
                     /gene="chuS"
                     /locus_tag="ACHJ7J_003472"
     CDS             18504..19532
                     /gene="chuS"
                     /locus_tag="ACHJ7J_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO5001658.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     MPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPASDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(19593..20123)
                     /gene="dctR"
                     /locus_tag="ACHJ7J_003473"
     CDS             complement(19593..20123)
                     /gene="dctR"
                     /locus_tag="ACHJ7J_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5001659.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILKKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(20279..20845)
                     /gene="slp"
                     /locus_tag="ACHJ7J_003474"
     CDS             complement(20279..20845)
                     /gene="slp"
                     /locus_tag="ACHJ7J_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO5001660.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         5554 a         4909 c         5056 g         5519 t
ORIGIN      
        1 ttgcgtacgc aagacataaa tatattctca ccatggttaa tggtgaatat cgctatttta
       61 atggcggtga cctggttttt gcggatgcaa gccaaattcg agtagataag tgtgttgaaa
      121 attttgtatt agtatcaagg gatacgcttt cattatttct cccgatgctc aaggaggagg
      181 cattaaatat tcatgcacat aaaaaagttt cttcattact cgttcatcac tgtagcagag
      241 atattcctgt ttttcaggaa gttgcgcaac tatcgcagaa taagaatctt cgctatgcag
      301 aaatgctacg taaaagagca ttaatctttg cgttgttatc tgtttttctt gaggatgagc
      361 actttatacc gctgcttctg aacgttttac aaccgaacat gcgaacacga gtttgtacgg
      421 ttatcaataa taatatcgcc catgagtgga cactagcccg aatcgccagc gagctgttga
      481 tgagtccaag tctgttaaag aaaaaattgc gcgaagaaga gacatcatat tcacagttgc
      541 ttactgagtg tagaatgaaa cgtgctttgc aacttattgt tatacatggt ttttcaatta
      601 agcgagttgc agtatcctgt ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa
      661 attattatgg gatgacgccc acagagtatc aggagcgatc ggcgcaggga ttgccgaacc
      721 gtgactcggc ggcaagtatt gttgcgcaag ggaattttta cggcgctgac tgttctgcgg
      781 aaggaataag attatagagt tttactcaga cataaaaaaa acccggcata ggggaccggg
      841 aagaggatag tctgccgtct ccagactaat aaaccgttat aacactccct gttggcacgg
      901 gaaactttgt gctctcagta agttaaatat aacttttact ggaaataaga tcagccattt
      961 ttttataaac ataagctata cgctgtgcga aaatatattc gtgctgcatt tacttattat
     1021 caattaactg ttatgcaaaa ctactttgtg gataaatttt ggtcctacca aatctggcag
     1081 tttttgcgct aagaaacagt ctggcatcat ttcattagta tactgaaatt gaaataatcg
     1141 cagtatgaaa tataagggat aatcatgact catgtctgct cggtgatcct cattcgtcgt
     1201 tcattcgata tttatcatga acagcaaaaa atatcgctgc ataacgagag tatcctgctg
     1261 ctggagaaaa atttggcaga cgattttgcg ttttgttcac cggatacgcg acggctggat
     1321 atcgatgagc tgacagtttg ccattactta caaaatattc gtcagctacc acgcaattta
     1381 gggttacata gcaaagaccg tctgttaatt aaccagtcac cccccatgcc gctggtgacg
     1441 gcgatttttg atagtttcaa tgactccggg gtaaattcgc cgatactgag caaaatgctc
     1501 tatctttcct gtttatcgat gttttctcat aagaaagaac tgatcccctt acttttcaat
     1561 agcatcagta ctgtttcagg aaaagttgaa cgccttatta gctttgatat cgctaaacgt
     1621 tggtatctgc gcgatatcgc ggaaagaatg tataccagcg agagtctcat caaaaaaaag
     1681 ttgcaggatg aaaacacctg tttcagtaaa atattactcg cctccaggat gtcgatggcc
     1741 agacgattac tcgagttacg tcaaattcct ctgcatacta ttgcggaaaa atgtggctat
     1801 agcagtacat cgtactttat aaacacattt cgacaatatt atggtgtaac gccacatcag
     1861 tttgcgcaac attcgccagg taccttttcc tgacatattt cgcatttgaa tattggtcag
     1921 gatctcacac ctgcttcatg tgaaactctt ccctgatgat ttctgccggg ctaccggctc
     1981 gttctctttc gcagtttgcc aggcggtata ggtattttca ggaggaaaag actcggcatg
     2041 tttgggatta ttaagctgac aattcatacg attacgggaa tgtgggtcag tattgtgctg
     2101 ttcaaattga tgacgaatgg ctggagtggt ttctactttc agtgctgtgt actaagcctg
     2161 gtattcctga cggttagctg gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct
     2221 gaaccttcac gttcaacctt actctcattt acacgttacg cttttttaaa gcgggcaaag
     2281 agatgttcca ctacaacgaa aaagaccgga acgaagtaaa ttgccagcac tgttgcggca
     2341 aacatcccgc ccatcacgcc ggtacctacc gcgttttgcg cgccagaacc agcaccatga
     2401 ctgataacca gcggcagcac gccgagaata aaggccagag aggtcatcag gattgggcgt
     2461 aaacgcatcc gcgccgcttc gatgattgcc tctatcggcg ttttcccttc tttctgcatc
     2521 atctcaacgg caaattcgac aatcaggatg gcgttcttgg cggaaagccc gatggtggtc
     2581 agcaaaccaa cctggaagta gacgtcatta cttaagccac gcagatcggt ggccagtaat
     2641 gcgccaacga cgcctaacgg aacaaccaac atcaccgaga acggaattga ccagctctca
     2701 taaagtgcgg cgagggcgag gaacaccacg accagtgaaa tggcatatag cgcgggagcc
     2761 tgatttgagg ataacgcttc ctgatacgac agtccggtcc atgagtagcc gacgcctgcc
     2821 ggaagtttag cgaccaggtc tgccataaat ttcattgcgt caccggtact tttcccggcc
     2881 gccgcttcac ctaaaatctc cattgacggg atgccgttgt agcgttccag tcgcggtgaa
     2941 ccataggtcc attcggtaga cgagtaggca gaaagcggtg ccatcgtgcc agaggcgttg
     3001 cgtacatacc attggttgat gttatccggc aacatacgga acggcgtgcc tgcctggaca
     3061 tacacttttt tcacccgccc ctggttgagg aagtcgttca cgtagctgct gccgaaggcg
     3121 gtggaaattg tctggttgat atcagacagc gccacaccca ttgcctcagc tttcgcggcg
     3181 ttgacgttca ctttgaacat cggcgtatct tccaggccgt tcgggcgtac cccggtgacc
     3241 tgattcggtg attgcgctgc cagtgataac agctcgtttc gtgcctgggt tagtttttcg
     3301 tgccccaggt taccgttgtc cagcagttcc atatcaaaac ctgacgcggt acccagttca
     3361 gccaccgcgg gtaagttgaa cgggaagacg acggctttat tgatactgct tagcgcaatc
     3421 attgcccgct gaatgatcgc ggtaaccgag ttttcctcac cgacacgttc agaccacggc
     3481 ttgagactga taaacgccag gccgttgttt tgcccctggc cgctgaagcc aaagccgcca
     3541 acggtaaaca ccgactggac attatctttc tctttagtca gataataatc cgtcacctgt
     3601 tgcagcactt tcgtggtgtt aaccatggtg gcaccggaag gtaactgcgc ggtggtcata
     3661 aataccccct gatcctcttc tggtaagaaa gaggtcggcg ttcgcaggaa cagcactgcc
     3721 atcccggcgc aaatcagcag gtagaccacc atgtagcgac cggtacaacg caacagcgag
     3781 cgggtgctat cggtatagtg ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgagc
     3841 agggcgttag gtttgtgacc gccttccggc gcggctttca gaatggtagc gcacagggca
     3901 ggggtcaggc tcattgccac aaatactgaa agcagcatgg aggagatcag cgtgatggag
     3961 aactggcggt agatctcccc ggttgcaccg ctcataaagg ccatcggcat aaacactgcg
     4021 gaaagaacaa cggcaatacc gaccagcgca cgttggatct gccccatcga tttatgcgtc
     4081 gcttccttcg gcggtagctt atcttccgca atgacacgct cgacgttctc caccactacg
     4141 atggcgtcat ccaccagtaa ccctatcgcc agcaccatcc cgaacatagt taacgtgttg
     4201 atggtgaaac cgaccgccga caagatcgca aacgtcccga gaataaccac cggtacggca
     4261 atcgtcggga tgattgtggc acggaaattc tgcaaaaaca gatacatgac caggaagacg
     4321 aggatgatag cctcaaccag tgttttgaaa acttcctgaa tagaaatttc gataaacggc
     4381 gtggtgtcgt aaggataaac cgtctttaga cttgccggga aataggctga taagcggttc
     4441 agctcctctt tgactgcccg cgaggtatcc agggcgtttg ctccggtagc cagtttgatg
     4501 gcgatcccgg cagcaggttt gccgttatag cgcgccacgg tggaataatc ttccgcccca
     4561 agttcgacgc gagcgacatc acgcagcagc acttgcgaac catcttgctg aactttcaac
     4621 aggattttgc caaattcttc cggcgtttgc agacgcgtct gcacaatgat cgaggcgttt
     4681 agctgctggt ctgccgcctg tggcatgcca cccagttgac caccagaaat ctggttgttc
     4741 tgcaccttaa tctgggaaat aacatcggaa ggtaccaggt tatatttatt gagtttttgc
     4801 ggatccagcc agatccgcat ggcgtactcg gaaccaaaga gctgtacgct accaacgccc
     4861 gcggtacggc ttagtgggtc tttgatatta gacgctacat agtccgcgat atcgtactgg
     4921 ttgaggctgc cgttatcaga aataaacgcc gctaccatca ggatattact gctcgattta
     4981 tcgacgctaa tcccctgctg ctgcactgct tcaggtaatg aaggcatagc gagttgcagt
     5041 ttattttgca cctgaacctg tgcgatatca ggagatgtcc cagtctcgaa ggtcagagtg
     5101 atagaggcat tacccgccgc atcactggtt gaagacatgt acatcaggcc atcaagccca
     5161 ttcatatttt gctcaatcac ctgagtgacc gagtcttcta ccgtttgcgc atcggcaccc
     5221 ggataggtag cgctgacggt aatggtcggt ggcgcaatct gcggatactg cgcaaccggt
     5281 aagttcatga tcgccagacc acctgcaagc atcataataa tggcaagtac ccaggcaaaa
     5341 accgggcgat caataaaata gttagccatg aaagtcccct taagcctgca acgttattgt
     5401 ttcgattcgg tgctggcgtt ttcctggctg gaggaaattg ctcgtgcttt gatacccgga
     5461 cgaatgcgtt gcaaaccgga aacgatcacc cgatcgccag cctgcaagcc agaggtgaca
     5521 acccactggt cgccgatggc tttgctggct tcaatttcgc gtagctgcac gacatcgtct
     5581 ttatccagaa tgagcgccgt tgctttaccc tgggcgttgt gggtgacgcc ttcctgcggc
     5641 accagtaata cattctggcg gctaccttca tccactaatg ccgtgacata catgccaggc
     5701 agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca
     5761 tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt accattttcc
     5821 agattgagct gtactggcgt actgccctga atctgtttga tttgcccact ggcgacctct
     5881 tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat cgggtccaga
     5941 cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc
     6001 gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg cagattgatc
     6061 gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc attcaactgg
     6121 gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg
     6181 cggttaaagg tgatgcgggc attactggcg gtagagagcg ctttcgccag cgagcctttg
     6241 gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga atcgccctgg
     6301 ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac ctggggacgt
     6361 atctcggcaa cttcataagg aacggttcta ccgggcaatt cgttcaacac attgaccgac
     6421 ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt ttccgccgat
     6481 ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa cagctttctt
     6541 cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta aattgcgcgc
     6601 gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag ccagtaaggt
     6661 gggatacagg catagtgatc gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct
     6721 gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat acacatacca
     6781 ggggaataaa gtcgttttag cctctccttt agtaatcacc gggggtaatg aaatcagcat
     6841 ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac taaaaataag
     6901 atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg tacataatgt
     6961 tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg atattgcttt
     7021 catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa aaacataatt
     7081 gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt ttgatacttt
     7141 ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg tgataaggtg
     7201 ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct tcttcatcaa
     7261 ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct acttttttga
     7321 tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc caaaagccct
     7381 gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta gccgttatcg
     7441 tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg ttcaatatag
     7501 taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca ctaatcaata
     7561 cttcagattt atccatcacg cactccatat attgatccta agtaagattc ctggttgtta
     7621 tcaacttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca agaataagtt
     7681 atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt aatccgattt
     7741 aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc tttcttttaa
     7801 cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta tatagcgtct
     7861 ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg aacccggatt
     7921 acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact ttcccacagc
     7981 acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt ttgaaatggc
     8041 taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat aatcactagc
     8101 aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc ggagagagta
     8161 ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg gataattatt
     8221 gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat atcagttcaa
     8281 ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgtagcacca aggaatatcc
     8341 aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct ttcattgaac
     8401 gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa ccggcaataa
     8461 atgccgcaat ggcaaaaatg cccagctccg gtgcgcggat gaagaaatag ccgatcaata
     8521 aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga ctgcggttgc
     8581 tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc actactgtgc
     8641 ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg ataaacagca
     8701 gcacggcaat aaactggatt gctctgcgat gttttttaag catctccaga tcaaacttca
     8761 aaattgttgc cttatctata tataacatag aaccacccta taaaattaag aagaaaatcc
     8821 cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa taaaaatttc
     8881 ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat atctgatttt
     8941 gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag tgtcgaaatt
     9001 gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa agtattaggc
     9061 gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga tgcgcaaaaa
     9121 gcagctgata acaaaaaacc gatcaactcc tggacctgtg aagatttcct ggctgtggac
     9181 gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa agataaacca
     9241 gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat cgttcaggct
     9301 tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg ggacaaaatt
     9361 aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata ttgaacaggc
     9421 cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc tatattgaat
     9481 gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg gcgctgtagc
     9541 ggctttgtca ctggtgaacg cacaatctgc gctggcagcc aatgaatccg ctaaagatat
     9601 gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg catggtggat
     9661 gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg aaaccgatct
     9721 cactcaaatt cctaaagtga tcgaatactg caagaaaaac ccgcagaaaa atttgtatac
     9781 cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta aaaaggagta
     9841 gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag aatttattat
     9901 acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc aaatgcgcgg
     9961 caaaggagca gggttacgca cacatgtatt aattggcatg ggaagcgccc tgtttatgat
    10021 tgtttcgaaa tatggttttg ctgacgtgct gtctttagat cacgtcggac tggaccccag
    10081 ccgtattgct gctcaggtag tgacgggcgt cgggtttatc ggtgcaggta acattctcgt
    10141 tcgtaaccaa aatattgtag gtctgacgac ggcagcggat atctgggtga ccgccgccat
    10201 aggtatggtt attggcagtg gtatgtacga actgggtatt tatggttcag tgatgacctt
    10261 gctggtgctg gaagtcttcc atcaattaac cttccgcctg atgaacaaaa attaccattt
    10321 acagctgaca ctagtgaacg gtaatacggt atcaatgctc gactggttta agcagcaaaa
    10381 aataaaaact gatttagttt cgttgcagga aaatgaagat catgaagtgg tcgccattga
    10441 tatccagctt catgccacaa cgtcgataga agatctgttg cgattattaa aaggtatggc
    10501 aggggtgaaa ggcgtttcaa ttagctaatc tgtatagtta tttattcgca ggagtaccag
    10561 tgcggtactc ctgttgcttt taatgatcga ggacgatcag tggtgaatgg ttggcaggat
    10621 ctttaagtac ggtaatatcc gcgccgtaga gcattgtcag cgcctgctgt gccaatacgt
    10681 cctgtggttt accatttgcg ataacctttc ctttttgcat taatacaaca cggtctgcat
    10741 aacgcgcggc aagatttaga tcgtgcaata tgcagcagac attgaactgc cgctcgtgaa
    10801 ctaactggcg taataaacga aacaagtgct gctgatggtg aatatccagc gccgaagttg
    10861 gttcatcaag aaataaccat ttcggtgagg gagtaggttc ccagagttgc accagcaggc
    10921 gcgctaattg cacgcgctgt tgttcaccac cggataattg ccgatagtcg cggtttgcta
    10981 acgcctgaca gtcacataac gccattattt gtgctgtttc attatcctga ttaccagtac
    11041 gatgcggatg ccgccccatc tggataactt cttgtacgct gaatggaaac gccatgtggc
    11101 tattttgacg catcacggcc cggtgtttcg ccagttcagt aatcgaccat tcatttaatg
    11161 gcttattaaa caaccgacat tcgccgctat caggttgaag atagccggtt aactggcgaa
    11221 gcagcgtaga ttttcccgca ccattagggc cgagaatggc aacaatttcc ccaccgggaa
    11281 aagtcagcga gacattatca gtcagacgcc gcccctgaag gctataaacc agattctggg
    11341 cgctgatcat gtgatcctcc gatttcgcag aatcaaccac ataaaataag ggccaccaag
    11401 caggctggtt aataatccca cgggcatttc tgctggctgt accagcgtgc gggcaagcgt
    11461 gtctgccatc agcaataaac aggctccggc gagggcggaa caagggatta gccagcggtg
    11521 atttgctccg gtagtcatgc gaatcagatg cgggatcacc aggccaataa agccgataat
    11581 gccgcttacc gatacggcag caccaacgag cagtgagctc actaacagta attgctggcg
    11641 tttacgctta acgttcacgc cgaggtaatg ggcttcttca tcaccgagct gcagtaaatt
    11701 cagcgttccg gcgagacaag ttgttgcgat aatggccggt aggatgaatg aactggcaac
    11761 caataacgtc ggccattgcg cctgtccaag attgcccatc atccacaatg tgagttgtcg
    11821 tagctgctgc tcatcgccga tatagctcag gatgccgatc gctgcgccgc acagggcatt
    11881 gatggcgata ccggcgagta ataattgcat catgctgcca tcgcgatggc gctgcgtgat
    11941 gagaaaaatg atggtgcaga ccactaaact tccggcaatg gcaaacacca tttgctcata
    12001 gagcaccaga accactggga aggagaaggg cagcacaata gcaacgccaa ccattaatgc
    12061 agaaccgcta ctgacgccaa gtaatcctgg gtcagccatt gggttgcgaa aaagcccctg
    12121 cataattacc cctgcggttg ccaacgctgc acctaccagc accgccagca aaacccgtgg
    12181 tagacgaata ttgagccaga tatcgcggta ttcctcatca aaaagggcat caaaactgat
    12241 ctttaacgct ccctgactag caccgaacag caccagggca agcaataata gtgatagccc
    12301 cataaagacc ctggcggatg aaaatgaatc cttgagcacg caaaactccg ttatgacgtt
    12361 gcggcgcgga gagtctgcgc cgcatgggat tacgccggtt tcaggtcagg ttcaatcaga
    12421 ctgtagatct gcttgttgag tgctggcgcg tttgccagtt catgaatgtg tgcagcgaca
    12481 tcggcacggc ggacaaaacc atgacactcc tgattttgta cccgttgtgc tttgcctgtt
    12541 gctgcgccgt caagcagacc accagggcgg agaatggcgt agtctagctg gctggtttgc
    12601 agccagcttt ccgccagtgt cttttcgcgc accgcctgac caaacgcggc ttttgcccga
    12661 tccgataaga acggccaact atcgccgcat cccaaagaag tcaccagaat catgcgactg
    12721 atgcctgcct tttcggcctc atcaatcact gttctgtgcg ccagataatc ctgagctcct
    12781 cccattgttg agatgataag cgcatccggg ccagctgcgc ggcaagcagc cgcaactacg
    12841 ctggcatcac aggcgtcgcc tgtaaaaacc tgtacgcctt gttgcgccag tttcgtggcg
    12901 gcatcggcat gacgaatgac agcaacgacc ggacgttgtt ccgccagcgc cagttcaagc
    12961 gttctggcac ccacaccttt tccgcctgcg ccaaagagta gccacggcgt catcaggcgt
    13021 gttcctttaa cgatgccgcc agagtatgga aggcgctgac ttgttcgctc aggagctgac
    13081 gatggtcatc cctgccgagg aagattttca gcatggcgct gccttcctta ttgaaaaaga
    13141 gaatggacgc ggtgtccatg cccataaatt tacgttcgat taaggcaata tgcgtgcagt
    13201 tttctgcttt gatatgcccg gacatgccgt gctttccgcg taggttgaaa tagccgtggc
    13261 gatggaaacc cgaaggcagt tcgccgctaa attcgaggat tacatcggca gtatgtacta
    13321 acgtggtgac gttgccccat tcgcagacgg tatcccagac agtatcgaat ttatcgcctg
    13381 acactatggt agaggagggc agatttttca ctacttccag cagtgtggtg ttgtattgtt
    13441 cggctaccac ctccagcgta ccgtccggct ccgttttcaa aaattcttgt aacgagacat
    13501 ggctcataaa tcactcctgt tatggttttt cacgcacaat cgctggcgca ttaagtacct
    13561 gaattagttc attaagagac tgcaaaatat tgcttgccca gaaacgacct tcattagtca
    13621 gccgcaggca agttgaagca tcgcgactta agcctttttg gtgccattga gccagtaacg
    13681 gcgcgagttt ttcggcatgt ggcgtaagtt catccagcgg tacacgcgct gtttcaacac
    13741 ctgactgcaa ggtatgacgc cattgcgcat tacgttcggc gttacgcatg atcatcatca
    13801 gcggtttttt gcctgccgcg acggattcat gccaggtctg aagattgcgt tcgttcatcc
    13861 aggagtaacc attaatcgaa ccgccggctc cggaaccaaa ggccagacaa tcggcacctt
    13921 gttttatcag caggttatag agattgcgtt cgcgtgtggt acggccccag tggctgttac
    13981 tgatgcagcg ccaaccggct tcgtccatga agtcacaccc ttgcagataa agatcgcgac
    14041 gttctgccgg agagggcacg gtagtacgcc cattttccac ggctttgccc agcggtgtat
    14101 tgggcaggac attgagcgca tagagatcga cgccatcgag accgatatcg cgggcaatag
    14161 ccagatcttc tccccaggtt tgcgcatcct gacccggcag gccaaacagc aggtcacaga
    14221 ccaccgcagc acggtcacgt ttgaccaggc tttccataaa cgcaatggcg gttgggccat
    14281 ccgaggtgcg ggccattttc ttgcggattt tgctgttaaa cgactgaata ccaattgaga
    14341 agcggtttgc tcctgcatca agacaaccat cgattcgctc agcgtcaaaa ttcagtaccc
    14401 ggccttcgat agtgatttca caatcagggg ccagtggcag cttttctcgt aatgtggtga
    14461 tgattctggc caaatcgtgt gccgaaagcg cggaaggcgt accgccaccg aaatagaccg
    14521 cgtggatggg ggcagactga tgcaatacgc tgtccgcttc catttctatt tcacgaatca
    14581 gggcgtcggt ataatgagca cacgcatctt cattaaaacg gttctgataa aaaccacaaa
    14641 acgtgcagtg tgtcgcacaa aaaggaatgt gtaggtaaac cagtcgctta cgtggtgata
    14701 ccgtttgatt gatcatctct tgccaggtct gtgccagttg ctctttggca actggaatgg
    14761 ccccacgaaa cggcatcatg gcgcgcctgt ctttaaatgg ctgatcaccg tctaaagcaa
    14821 aatgtggcgt gagatccagg gtgttatttg cgttcattcg tctgatttaa atccatttat
    14881 tgcggccaaa gctgctggtg taaagactca acgacatcag caatacgcgg acccattccc
    14941 agaatcaggt tttgatcaac ggtgataatg cgctggtttt tccatgcggc agtgtgggta
    15001 attccagcaa tagaacgtag ccgattaata tcgccatcga ccatttgtga ggttacgaca
    15061 atcacttcag ggttagccgc aatcagtgat tctgcactgt agcttttgta ctgctggtgg
    15121 gttgcgacgt tttctgctcc ggcaagcgaa agaatggcgt ccgcgacgct gcctttaccg
    15181 gcaacctgtg gcgcacttcc gcctgcggag agaataaaca tggctttaac cggggctttt
    15241 ttggccgcca cgttttgctg tactcgctcc aggcgttggc tgatttgtgt caccagcgcc
    15301 tcgccttgtt caggaaactg tagcgttttt gccagttggc gaatgttggc gtacatctgc
    15361 tcaagggtgg ctggcacacg cggcagggtg acgacgttga ctttctgcgc tcgtagttgg
    15421 tcgagcacaa tttgtggccc ggcatcctgc caggtaatta cgctatccgg gcgaagtgac
    15481 aaaatgcctt cgctgctgag ttgtttccag tagccaatat gcggcagttt ggcggtttct
    15541 ggtggataag atgtcgtttc atcgacacca accacgcgtt cgccagcgcc catcgcgtag
    15601 attagctccg tcagcgatcc tcctgcgacc acgatacgtt cggcagccgt tacgctaaaa
    15661 gcccagctaa gagccagtac tgccgtaaac aatttttttc gttttactgc ggtcttcgcg
    15721 gcagaggcca cggctgaaat acataacgtc agtggggaaa aaagaaacgg cctggtgatg
    15781 atccttggca taatatgaga actcttatca aattgagaat gatcatgatt atcatttgag
    15841 ttatatatga aatacaaccg tcgattcaat acgcaaggcg ttacaagcgt atttattttt
    15901 gatttttatc aagatgcttc gtcagctaac gttcttgtta ccgactaaat ttcatcgcgg
    15961 gaaaaagctg agaacccaga gatatcgagg cttgcagagc aaagagaaga aaaaattatc
    16021 actgttaacg acaaaattta gtgatgaggc atggggggat aaccataaaa acaaggtggt
    16081 ttacagggcg caggataggt ttcataaccc cctgtttatt gtgttaatgg tggttaaaaa
    16141 aaacttgatc ataaaaaatt gatcgataat aattctcatt atcacgtttg catttaacaa
    16201 aacacgacca tatggataac aaaaccatag catttgcaag ggataacgca cagctctctt
    16261 cactcctgat ggggcatatc gttttgcctg acaaagaggg cgtgcttttg cgtaattgcc
    16321 gagttatcag gcaatttcat gggatataaa cgcctgtaaa ctcacccggc gaatattatc
    16381 gctctgaatt ttaaataaat ttatttttcg ccagccaccc cggtattaac ccaggcagcc
    16441 agcatttttt attttcctca tggagaatcg ctatgtcacg tccgcaattt acctcgttgc
    16501 gtttgagttt gttggccttg gctgtttctg ccaccttgcc aacgtttgct tttgctactg
    16561 aaaccatgac cgttacggca acggggaatg cccgtagttc cttcgaagcg cctatgatgg
    16621 tcagcgtcat cgacacttcc gctcctgaaa atcaaacggc tacttcagcc accgatctgc
    16681 tgcgtcatgt tcctggaatt actctggatg gtaccggacg aaccaacggt caggatgtaa
    16741 atatgcgtgg ctatgatcat cgcggcgttc tggttcttat cgatggtgtt cgccagggaa
    16801 cggataccgg acacctgaat ggcacttttc tcgatccggc gctgatcaag cgtgttgaga
    16861 ttgttcgcgg accttcagca ttactgtatg gcagtggcgc gctgggtgga gtgatctcct
    16921 acgatacggt cgatgcaaaa gatttattgc aggaaggaca aagcagtggt tttcgtgtct
    16981 ttggtactgg cggcacgggg gaccatagcc tgggattagg cgcgagcgcg tttgggcgaa
    17041 ctgaaaatct ggatggtatt gtggcctggt ccagtcgcga tcggggtgat ttacgccaga
    17101 gcaatggtga aaccgcgccg aatgacgagt ccattaataa catgctggcg aaagggacct
    17161 ggcaaattga ttcagcccag tctctgagcg gtttagtgcg ttactacaac aacgacgcgc
    17221 gtgaaccaaa aaatccgcag accgttgagg cttctgaaag cagcaacccg atggttgatc
    17281 gttcaacaat tcaacgcgat gcgcagcttt cttataaact cgccccgcag ggcaacgact
    17341 ggttaaatgc agatgcaaaa atttattggt cggaagtccg tattaatgcg caaaacacgg
    17401 ggagttccgg cgagtatcgt gaacagataa caaaaggagc caggctggag aaccgttcca
    17461 ctctctttgc cgacagtttc gcttctcact tactgacata tggcggtgag tattatcgtc
    17521 aggaacaaca tccgggcggc gcgacgacgg gcttcccgca agcaaaaatc gatttcagct
    17581 ctggctggct acaggatgag ataaccttac gcgatctgcc gattaccctg cttggcggaa
    17641 cccgctatga cagttatcgc ggtagcagcg atggctacaa agatgttgat gccgacaaat
    17701 ggtcatctcg tgcggggatg actatcaacc cgaccaactg gctgatgtta tttggctcat
    17761 atgcccaggc attccgcgcc ccgacgatgg gcgaaatgta taacgattct aagcacttct
    17821 cgattggtcg cttctatacc aactattggg tgccaaaccc gaacttacgt ccggaaacta
    17881 acgaaactca ggagtacggt tttgggctgc gttttgatga cctgatgttg tccaatgatg
    17941 ctctggaatt taaagccagc tactttgata ccaaagcgaa ggattacatc tccacgaccg
    18001 tcgatttcgc ggcggcgacg actatgtcgt ataacgtccc gaacgccaaa atctggggct
    18061 gggatgtgat gacgaaatat accactgatc tgtttagcct tgatgtggcc tataaccgta
    18121 cccgcggcaa agacaccgat accggcgaat acatctccag cattaacccg gataccgtta
    18181 ccagcactct gaatattccg atcgctcaca gtggcttctc tgttgggtgg gttggtacgt
    18241 ttgccgatcg ctcaacacat atcagcagca gttacagcaa acaaccaggc tatggcgtga
    18301 atgatttcta cgtcagttat caaggacaac aggcgctcaa aggtatgacc actactttgg
    18361 tgttgggtaa cgctttcgac aaagagtact ggtcgccgca aggcatccca caggatggtc
    18421 gtaacggaaa aattttcgtg agttatcaat ggtaatcatc tgccccgata tttcggggca
    18481 tttatctgga aggaagagag acaatgaacc actacacacg ctggcttgag ttaaaagaac
    18541 aaaatcccgg aaagtacgcg cgtgacatcg cagggttaat gaatattaga gaagcagaac
    18601 tggcatttgc acgcgtcacg cacgatgcgt ggcggatgcg cggcgatatc cgtgaaattc
    18661 tggcggcgct cgaaagtgtt ggcgagacca aatgtatttg ccgtaatgaa tatgcagtcc
    18721 atgagcaagt tggtacgttc acaaaccagc atttgaacgg acatgccgga ttgatcctca
    18781 atccgcgcgc gctggattta cgtctgtttc tcaatcaatg ggccagtgtt ttccacatca
    18841 aagaaaacac ggctcgtggc gaacgccaga gtattcagtt ctttgatcat cagggtgatg
    18901 cattactaaa agtttatgcc accgacaata ccgatatggc ggcatggagt gagcttctgg
    18961 cacggtttat caccgatgag aatatgccgc ttgagttaaa agccgttgat gcgccagttg
    19021 ttcaaacgcg agccgatgcc actgtggtcg agcaagagtg gcgggcgatg accgacgttc
    19081 atcagttttt tacgttgcta aagcgccaca atctgacgcg ccaacaggcg ttcaatctgg
    19141 tggcagacga tttggcctgc aaagtatcca acagtgcgtt ggcgcaaatt cttgaatctg
    19201 cacagcagga tggtaatgaa atcatggtgt ttgttggcaa ccgtggctgc gtacagattt
    19261 tcactggtgt ggtagaaaaa gtggtgccaa tgaaaggttg gctgaatatt ttcaacccga
    19321 cgtttactct tcatctatta gaagagagca ttgctgaagc ctgggttacc cgtaaacctg
    19381 ctagtgacgg ttatgtgacc agtctggaat tgtttgctca tgatggtacg cagatagcgc
    19441 aactttatgg tcaacgtaca gaaggcgaac aggagcaagc gcaatggcgt aagcaaattg
    19501 cttcgctaat accggaaggc gttgctgcat aacgtataat taacgggagg tctggctatt
    19561 tattgctggg cctctttata ttgatcaatc gttcacacca gataatcaat atgctgatgg
    19621 cgtaccagct cattgatccg cttaagattg aggatcatca tgatattgtg tttatggcag
    19681 tagaacgttt ttagcgatat tttcagtttc ttcagaatat cttccgttgt catttgattc
    19741 gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg acacattcgc ttcaggcaca
    19801 gagcagtaac tgccattgat gagtgcgaca aaatcaatga tagtgatagt ccgcggaaca
    19861 aaggtcaccg gcactttccc caggcagcgt ttaatattaa agggggagaa aataatgaca
    19921 tggacaggtt ttaatcgttc aatttgagtg agaaaattag aatgaaatac attattcatt
    19981 aatgtatcaa taatgacgaa ggcattctga tgcaacatta cgtcgatctc ttcttcgttc
    20041 tgtatatgaa cgacattacc tttactcaga atgtttttca tcgcggtgaa gaacatcgta
    20101 tccctggtaa ttataagaaa cataaccgac tcctggtgcg gacattaact ttataatcag
    20161 tttgccaaag gtaattaaca ggacagggct tataataata atagttagct tactgtatat
    20221 taattttatg taatacatga tgcagatgct gagaaagcat ctgcatcttt cggtggtgtt
    20281 atttgaccag ctcaggtgtt acctgactca ccgcattggt gtagtaaggc gcaccccaac
    20341 ccggttccgg ccagaatgca ccatagccgt aatcccacag gttataggtg gtattaacta
    20401 cttctctcaa atgccacacc tggatgccct gcatattcac ttccaggaag ttatacggga
    20461 ctttattgat aaagcctggt tgttcaccct gaatggtacc gaggatggta acaaagtgat
    20521 tacgatagtt cactgggtca aggaagccgc tttgtctggc gagcagtcgg ccctgatagt
    20581 tggcttcaat atcaggcttc gcatagctat ccaacggtaa tacagcgatt tctaacaacg
    20641 tatccgtttt gccattgata acgttgataa ccttcccacc aaagcgcgct tgttgaccaa
    20701 catataaccc cggctggtta tgaacagcaa caaaactttt ttgaatatca ggttggttat
    20761 tgcctttgat attttgcgga attgaactac atgcggcaag caaaaatgaa aggctgagta
    20821 tgagtgcacc ttttgtcatg ttcatgttac tatccttatc aacaaatatt aaaaccataa
    20881 tagatatttc tttatagttt tcatctgatt ctgaggcgat atgcctgata gtaacgtaaa
    20941 cctgtctgat gtttatatcc cttctgttta gttcaattcc gttattttgg ggcgtggcat
    21001 gagtatccat cgccccataa tataaccctt tgttttaa
//
LOCUS       DAZFFG010000086        19744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFFG010000086 DAZFFG010000000
VERSION     DAZFFG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.086"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            100..603
                     /gene="tssB"
                     /locus_tag="ACHJ7J_003475"
     CDS             100..603
                     /gene="tssB"
                     /locus_tag="ACHJ7J_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5001661.1"
                     /translation="MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNL
                     KGKPDNTPLDERTAIAINRYNLNAVISEADIEREFVAPAELSDAPNEQMYVNLKVKSM
                     DDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDSREQLIQE
                     LGIAVQK"
     gene            626..2110
                     /gene="tssC"
                     /locus_tag="ACHJ7J_003476"
     CDS             626..2110
                     /gene="tssC"
                     /locus_tag="ACHJ7J_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5001662.1"
                     /translation="MSVKEDIAPVSASQTAAPPSLLDEIMAQTRVQPKSESYDITRQG
                     VSAFIAAMLQGDSSAEPINILAVDAMIADIDARTSRQMDAIIHAPEFQELESLLRSLK
                     LLVERADTRENIKVHFLNVTQEELLDDFEFAPEITQSAYYKHVYSSGYGQFGGEPVAA
                     VIGNFAFKNTTPDMKLLKYISQVSAMAHSPFLSSVSSEFFGLDSWTELPGIKEPGAIF
                     EGPAYSRWRALRESEDSRYLGLTAPRFLLRHPYSPDENPARTFRYHEDVSQSHESYLW
                     GNTSFLLAANLAESFAKYRWCPNIIGPSSGGAVKDLPVHLYESMGQMQAKIPTEVLIT
                     DRREYELAEEGFITLTMRKGSDNACFFSANSVQKPKTYPKTPEGKAAETNYKLGTQLP
                     YLFVISRLAHYIKVIQREQLGSWKERSDLERELNTWIRQYVADQENPPAEVRSHRPLR
                     QAKIEVLDVDGEPGWYQVAISVRPHFKYMGASFDLSLVGRLDKE"
     gene            2115..2540
                     /gene="tssE"
                     /locus_tag="ACHJ7J_003477"
     CDS             2115..2540
                     /gene="tssE"
                     /locus_tag="ACHJ7J_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5001663.1"
                     /translation="MQRRDSGPTSSLFERIREAANPPSYQNPKEALIRSIRRNLRQVL
                     NTRSGSCYGSPELGITDLNDESLASSDFRREIRKSISQCILHYEPRITDVVVTAAAPD
                     EYAPVELRFHIVATVDVSETRGVFEFDILLDNHQRYCVE"
     gene            2546..4387
                     /gene="tssF"
                     /locus_tag="ACHJ7J_003478"
     CDS             2546..4387
                     /gene="tssF"
                     /locus_tag="ACHJ7J_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5001664.1"
                     /translation="MAFEERYYREELDYLRQLGKLLAQEKPHLAHFLAEKEGDPDVER
                     LMEAFAFMSGSLRQKLEDEFPEFTHGLIRMLWANYLRPVPAMTVIAYEPKTDQLKVPV
                     QVCRNELIRSRSEKSALAAQKVLSDNHDKIVSSVACHFTLARDIWLQPLRILDTRNAS
                     SLKEGIIDISFTADNNVSPAMLDLNKITFWLGNEDDYTRHQLYLWFCECLMDAELIAG
                     EYRLPLPDLWLEAAGFENQDALLPWPKNVHSGYRVLQEYFCYPEAFFFFHLRDVAPLP
                     DDFPVSAFTLRLHFNRPLPADIKLRQDSMRLHCTPAINLFTHYAEPVRPDGRIAEYPL
                     RASHRHPDAYDIFQISTVTSKVKVSGTESNPGSQARVWPEFESFQHQMEYSRQREVVY
                     WHHRTKTSLFHRGLEHSIAFVHADGSIPDSSRFNDDVITTSLICTNRMIPARLHIGDI
                     CVAVNKNPAVASFHNVTRPTQPLWPVTDGDMHWSLISAMNLNYLSLLDRETLIQILRT
                     FDLPGAHHPQRARLSRQKLDAIEKLETKPVDRLFKGVPVRGLATTLWIRPDPFICEGE
                     IYLLGTVLSHFFALYASINSYHCLKIINTESQEAWEWQEKMGQHALI"
     gene            4351..5400
                     /gene="tssG"
                     /locus_tag="ACHJ7J_003479"
     CDS             4351..5400
                     /gene="tssG"
                     /locus_tag="ACHJ7J_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5001665.1"
                     /translation="MAGENGSARPYIAVKPPLSADVRNCNFYVLMEALYRRYGAPGQD
                     ISLRTEPAQEIVRFSSDANIGFPGTDLSVLSRSQNGQYVLQTRFLGFSGSQSPLPGYY
                     LDQMAQESAQNEDGLKEFLDLFSHRWTQFAYHAWRKYRYYICFRNGGNDTFSQRMYAL
                     VGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRK
                     VAIDPAHQNRLGVRNPKGKTAGHIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRER
                     YLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRLGDPASSRLGRTSFI
                     GQPKVPPAITIRIRE"
     gene            5417..6697
                     /gene="tagH"
                     /locus_tag="ACHJ7J_003480"
     CDS             5417..6697
                     /gene="tagH"
                     /locus_tag="ACHJ7J_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001511117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO5001666.1"
                     /translation="MAEEKQQSRQASLTLQVMNGNELESGRAAKCLFSENGGDIGHTP
                     ECHWQVQDRAGSIAARACTVIRHDGAYCLRCLTPGLMINLAPVSSDALIRLRQGDEIQ
                     LGALALKAFLHDGAAVTYDERMATPETIVMNRDSLADTLLTTEGQPSYPGMPFQHQLA
                     PTVAHGFSSDPLQALQTESLMVADDPIAPRVSRPVAPASELTGANGINTPFMDLPPGN
                     ARYEDDDEFSAMAQYHLAVTPLLRGMECPLTLHDSQDADEFLEEAGRALQAAIKGLLS
                     LQQQQNSLSDKHLRPLEDNPLRLDMDYATALNVMFAEGKSPVHLAAPAAIGESLRNIR
                     HHEEANRAAIVEALRVMLEAFSPGNLMRRFAQYRRGHELRQKMDDAWAWQMYSNYYDE
                     LASSRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            6694..7218
                     /gene="tssJ"
                     /locus_tag="ACHJ7J_003481"
     CDS             6694..7218
                     /gene="tssJ"
                     /locus_tag="ACHJ7J_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5001667.1"
                     /translation="MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMDQST
                     ELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFQAADYDQVATTALPDVLGKNYI
                     DHQDFNLLPDTVKTLPPIKLDEKTSYIGVIAYFSDDQATEWKQIEPVESIGHHYRLLV
                     HIRASAIEMKKEEN"
     gene            7221..8567
                     /gene="tssK"
                     /locus_tag="ACHJ7J_003482"
                     /pseudo
     CDS             7221..8567
                     /gene="tssK"
                     /locus_tag="ACHJ7J_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            8614..9345
                     /gene="icmH"
                     /locus_tag="ACHJ7J_003483"
     CDS             8614..9345
                     /gene="icmH"
                     /locus_tag="ACHJ7J_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO5001668.1"
                     /translation="MEPESGTSGRQYRLALRGNSLNPMIDAATPLLGMVMRLSTMNSQ
                     AMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIVSFRYILCTFIDEAALGNGWSNKNEWI
                     KQSLLVHFHNEAWGGEKVFILLERLIREPIRYQDLLEFLYLCFSLGFRGRYKVAIQQQ
                     DEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIRHVMCGGLVVLAV
                     FYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9355..12087
                     /gene="tssH"
                     /locus_tag="ACHJ7J_003484"
                     /gene_synonym="clpV"
     CDS             9355..12087
                     /gene="tssH"
                     /locus_tag="ACHJ7J_003484"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5001669.1"
                     /translation="MIQIDLATLVKRLNPFAKQALEMAASECMSQQASEITVAHVLLQ
                     MLAIPRNDVRVIAERTGISAEDLRQALTVESYPGGRSADGYPSFSPMLIEWLKESWLL
                     ASAQMQHSELRSGVLLLTLLHSPLRYIPPAAARLLTVINRDQLQQDFAAWTKESAESV
                     DLAGGQTPRATETSDTLLARYAKNMTADARNGKLDPVLCRDHEIDLMIDILCRRRKNN
                     PVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEKRF
                     KGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATTWS
                     EYKKYFEKDAALSRRFQLVKVSEPSAAEATIILRGLSSVYEQSHGVLIDDDALQAAAT
                     LSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLEREL
                     RIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQLLLGVTEDTDA
                     VVDTPPESEQDNTGTAPADDAGSTQPEETAETVSPVQQLVQLTAELDALHNDRLLVSP
                     HVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLAIASLHKHLLTA
                     RADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKHTVSR
                     LIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEMADGE
                     GRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALLARMEVVPYL
                     PLSKETLATIIAGKLARLDNVLCSRFGAEVIIEPEVTDEIMSRVTRAENGARMLESVI
                     DGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDAQDDEFVTKDETVL"
     gene            12084..12827
                     /gene="vasI"
                     /locus_tag="ACHJ7J_003485"
     CDS             12084..12827
                     /gene="vasI"
                     /locus_tag="ACHJ7J_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO5001670.1"
                     /translation="MSRLRVFRQAGWFIAGLMAGLPASAAPAETSSMAGAAVAVATTP
                     PPDATATLQAMQSCRRESAALERLDCYDRILAPEQPGFGNAALVKASFVGEAWTRATE
                     QEKRRQGNTTELLATQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARVIAPLRDACHWAGE"
     gene            12832..14274
                     /gene="tssA"
                     /locus_tag="ACHJ7J_003486"
     CDS             12832..14274
                     /gene="tssA"
                     /locus_tag="ACHJ7J_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO5001671.1"
                     /translation="MAMDLRNPDVWLAHLLENLPEDKLSAALDDGNADWEFIDSEIVK
                     LGSLAHSQLDIPELQRRGLMLLASETKDFRLLAHLLRTLQHAGDILLASRMLAQYTEH
                     YWTCAAPQNMAHKKRFAAQVIKRFESAVQDFAGNAATTQRDALLGELAKLAQCWQAHN
                     APELAKATDDLFALFQRAFRDVAPEMPSSARTAASAPQATSRSVPDMSPHTAAAQPPV
                     AATPIPQVIVDSHDDKAWRDTLLKVAAILCERQPESPLGYRLRRHALWQTITSTPQAE
                     SDGRTPLAAFPVDMMDDYLARLNNADMALWQQVEKSLLLAPYWLDGHYLSAQTALRLG
                     YIQVADAIRDEVTSFLARLPALINLLFNDRTPFVSEQTKQWLAPSGSGNQTAAAVQTD
                     EELQAARACFDENGLETALRYLENLPEGDPRNQFHRQFFGAQLLEEAGMVQLAQQQYR
                     MLFKTGLHLMLPEWEPSLLKALEQKLTAEQ"
     gene            14384..17821
                     /gene="tssM"
                     /locus_tag="ACHJ7J_003487"
     CDS             14384..17821
                     /gene="tssM"
                     /locus_tag="ACHJ7J_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO5001672.1"
                     /translation="MLALAWIFLLVWIWWQGPKWTLYEQHWLAPLANRWLATAVWGLI
                     ALVWLTWRVMKRLQKLEKQQKQQRDEEKDPLTVELHRQQQYLDHWLLRLRRHLDNRRY
                     LWQLPWYMVIGPAGSGKSTLLREGFPSDIIYTPESIRGVEYHPLITPRVGNQAVIFDV
                     DGVLTSPGGDDLLHRRLREHWLGWLMQTRARQPLNGLILTLDLPDLLTADKPRREALV
                     QNLRQQLQEIRQCLHCRLPVYVVLTRLDLLTGFAALFHSLDKKDRDAILGVTFTRRAH
                     ENDDWRSELSTFWQTWGQQMNLALSDLMLVQTGAALRSAVFSFSRQMQGTGEIVTALL
                     AALLDGENMDVMLRGVWLTSSLQRGQVDDIFTQSAARQYGLGNSSLATWPLVETTPYF
                     TRRLFPEVLLAEPNLAGENSVWLSSSRRRLTAFSACGAALAALLVGSWHHYYNQNWQS
                     GVNVLAQAKAFMDVPPPQGMDEFGNLQLPLLNPVRDATLAYGDYRDHGFLADMGLYQG
                     VRVGPYVEQTYIQLLEQRYLPSLMNGLIRDLNNAPPESEEKLAVLRVLRMMEDKSGRN
                     NEAVKQYMARRWSNEFHGQRDIQAQLMAHLDYALEHTDWHAQRQSGDSDAVSRWTPYD
                     KPVINAQHELSKLPIYQRVYQTLRTKALSVLPADLNLRDQVGPTFDNVFVAGNDEKLV
                     IPQFLTRYGLQSYFVKQHDGLVELTALDSWVLSLTQSVAYSEADREEIQRHITEQYLS
                     DYTATWRAGMDNLNVRDYEAMPELTDALEQIISGDQPFQRALTALRDNTHALTLSGKL
                     DDKAREAAINEMDYRLLSRLGHEFAPENSALEEQKDKASTLQAVYQQLTELHRYLLAI
                     QNSPVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGKLADQAWHVVMVE
                     AVHYMEVDWRDNVVKPFNEQLAGNYPFNPRAPQDASLDAFERFFKPDGILDTFYRDNL
                     RLFLENDLTFGDDGRVLIREDIRQQLDTAQKIRDIFFSQQNGLGAQFAVETVSLSGNK
                     RRSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGGAPRSIAFSGPWA
                     QFRLFGAGQLTNVTSDTFNVRFNVDGGAMVYRVHVDTEDNPFTGGLFSQFRLPDTLY"
     gene            17832..19190
                     /locus_tag="ACHJ7J_003488"
     CDS             17832..19190
                     /locus_tag="ACHJ7J_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO5001673.1"
                     /translation="MNNNALTQTIVTGSDPRALPEFSAIREEINKANHPSQPELNWKL
                     VESLALSIFKANGVDLHTATYYTLARTRNQGLAGFCEGAELLAAMINHEWDKFWPQGG
                     PARTEMLDWFNTRTGNILRQQVSFSENDLPLLYRTERALQLICDKLQQVELKRQPRVE
                     NLLYFVQNTRKRFEPQPGNRTDTAAQTTVRTLVYAPESTASTTAETIPPLPDLPEMRV
                     EVRGVADNADKAKQGDTVKGFVAGAACTAVIAAALWWWQTYPMQRQLTQVRDTAQGAA
                     TVWLASPVLKEYEQNLQQLLNTPPLQPLETGMQMMRTADTLWPESLQQQEASRMWSTT
                     LRNRAQASPQMKGWQQARQNLRDFADLMMKKETEKQGFTLSYIKTVTWQAERLLNQET
                     PLEYMLTQYQETRTQNQDTQALAKEINQRLDGLLSRWLLLKNTGQDMATDNSTVPVPP
                     TH"
     gene            19208..19687
                     /locus_tag="ACHJ7J_003489"
     CDS             19208..19687
                     /locus_tag="ACHJ7J_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5001674.1"
                     /translation="MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVTSL
                     SHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENECLTCDFVFYRTNRFGINEPYYK
                     LKLTNARISNIGLTVPHTINDSPGQPEESVSFTYESINWEHSVAGTSAYSLWSERIF"
BASE COUNT         4571 a         5610 c         5492 g         4071 t
ORIGIN      
        1 aaggaaggtt acattatcta actgccactg aaacggtttc ttcggtattt ttatgcagta
       61 cgaaaatgct gtgctcatgg cctgaacggg aacattttta tgagcaaaat gaacaacaat
      121 ggcggcagtg tcgcgccgaa agaaagaatc agcgttcggt atacaccaaa agttgatggg
      181 gtggctgctg atatcgaatt gccgctgaat ttattgatca ccggcaatct gaaagggaaa
      241 ccagataaca cgccgttgga cgagcgaact gccattgcta tcaaccgcta taacctcaac
      301 gcagtcattt cagaagctga cattgaacgg gagtttgttg ctccggctga actcagtgac
      361 gctcccaatg agcaaatgta cgtcaacctg aaagtcaaat ctatggatga cctttctccg
      421 gaccatattg ccagccaggt accagaaata aaacgtctgc tcgaattgcg tgaagctctg
      481 gtcgcactta agtctccact aggcaatatt ccggccttcc gcgcacaact tcaggcgcta
      541 ctggaaaacg aagacagccg cgagcagctg atccaagaac tgggtatcgc cgttcagaaa
      601 taattttcac agaaggattt tcagtatgtc agtaaaggaa gatattgctc ccgtcagcgc
      661 gtcgcaaacc gccgcgcccc catctctgct tgatgaaatc atggcgcaaa cccgcgtcca
      721 gcctaaatca gaaagctacg atatcacccg ccagggcgtg agcgccttta ttgccgccat
      781 gttacagggc gacagtagcg ctgaacctat caatatcctg gccgttgacg ccatgattgc
      841 tgatattgat gcccgaacca gcaggcagat ggatgccatt attcatgccc cggagtttca
      901 ggaactggaa tccctgctgc gctcgctgaa gctgctggtt gaacgcgcgg atacacggga
      961 aaacatcaag gtccatttcc tgaatgtcac tcaggaagaa ctgctggatg atttcgaatt
     1021 tgcacctgaa atcacgcagt ctgcatatta caagcatgta tattcaagcg gttacggaca
     1081 atttggcggt gagccagtgg cggcggtaat cggcaacttt gcctttaaga acaccacgcc
     1141 ggatatgaag ttgctgaaat atatcagcca ggtcagtgcg atggcgcaca gcccgttcct
     1201 ctcatcagta tcatccgaat ttttcggtct ggactcatgg acagagttac cggggattaa
     1261 agaaccagga gccatctttg aaggcccggc ctattcccgc tggcgtgcct tgcgtgaatc
     1321 agaagattca cgctacctgg ggctgaccgc cccccggttc ctgctacgtc acccttactc
     1381 gccggatgag aacccagcca ggaccttccg ttaccatgaa gacgtcagcc agagtcacga
     1441 gtcttatttg tgggggaaca cctcgttttt actagctgcg aacctggctg aaagttttgc
     1501 taaatatcgc tggtgtccca atatcattgg cccttccagc ggcggcgcag tcaaagacct
     1561 cccggtgcat ctgtatgaat ccatggggca aatgcaggca aaaattccga ctgaagtgtt
     1621 gatcactgac cggagagagt atgagctggc ggaagaaggc tttatcacac tgaccatgcg
     1681 taaagggtct gataatgcgt gctttttctc ggccaactcg gtgcagaaac ccaaaacata
     1741 ccccaaaacc cccgaaggca aggctgcgga gaccaactat aaactcggca cccagctacc
     1801 atacttattt gttatcagta ggttagcgca ttacatcaag gtaattcagc gcgaacagct
     1861 tggctcatgg aaagaacgca gtgacctgga gcgcgagctg aacacctgga ttcgccagta
     1921 tgtggccgac caggagaatc cgcctgccga agtccgcagc caccggccat tacgtcaggc
     1981 gaagatcgag gtacttgatg tggatggtga gccaggctgg taccaggtcg cgatttctgt
     2041 cagaccgcat tttaaataca tgggtgccag tttcgacctg agcttggtcg gacgactgga
     2101 taaggaataa tcagatgcaa agacgggatt cagggccaac cagcagtctt tttgaacgta
     2161 tccgtgaggc ggccaaccca ccttcatacc agaatccaaa agaagcactg atacgttcca
     2221 tcagacgtaa cctgcgacag gtcctgaata cccgctccgg cagttgttat ggctcaccag
     2281 agctggggat tactgattta aatgatgaat cgctggcctc cagcgatttc agacgagaaa
     2341 tccgtaagtc cattagccag tgcattttac attacgaacc tcgtattacc gatgttgttg
     2401 ttacggctgc ggcaccggat gaatacgccc ctgtggaact acgttttcat attgtggcta
     2461 cggtcgatgt cagcgaaacc aggggagtgt ttgaatttga cattttgctg gacaatcatc
     2521 aacgctattg tgtggagtga tctctgtggc ttttgaagaa cgttactacc gcgaagagct
     2581 ggattatctt cgccagcttg gcaaactgct ggcgcaggaa aaaccacatc tggcccactt
     2641 tctggccgag aaagagggtg atccggacgt ggagcgtctg atggaagcct ttgcctttat
     2701 gtccggtagc ctgcgtcaga aacttgagga cgaattcccc gaattcaccc atgggctaat
     2761 ccgtatgctg tgggcaaact atttacgccc agttccagcc atgacggtta ttgcctatga
     2821 acccaaaacc gatcagttaa aagtccctgt tcaagtctgc cgtaatgaac tgatcagaag
     2881 ccgctcagaa aagtccgccc tggctgctca gaaggtgctg tctgacaatc atgataaaat
     2941 cgtttcttcc gtagcctgtc atttcacgct ggcgcgcgat atctggctgc aaccactgcg
     3001 cattctcgac acccgcaacg ccagcagcct gaaagaaggc atcatagata tcagttttac
     3061 cgcagataac aatgtctccc ccgccatgct tgacctgaac aaaataacgt tctggctggg
     3121 taatgaagac gattataccc gacaccagct ttatctgtgg ttttgcgaat gcctgatgga
     3181 tgcggaactg attgccggag agtaccgcct gccgctgccg gatctgtggc tggaggccgc
     3241 cgggtttgaa aaccaggacg ccctgctgcc gtggccgaaa aatgtccaca gcggctatcg
     3301 ggtactccag gaatatttct gctatcccga agccttcttc ttttttcatc tgcgcgatgt
     3361 tgcgccgcta ccagacgatt tcccggtcag cgcgtttacg ctgcgcctgc actttaaccg
     3421 cccgttacct gccgacatca agctgcgtca ggactcaatg cgtctgcact gtacgccagc
     3481 cattaacctg tttacgcatt atgcggaacc cgtcaggcct gatggacgga tagcggaata
     3541 cccgctgcgc gccagccacc ggcatcccga cgcttatgac atttttcaga taagcacagt
     3601 caccagcaaa gtcaaagttt caggtactga atccaaccct ggcagccagg cgcgtgtctg
     3661 gcccgaattt gaaagtttcc agcaccagat ggagtacagc cgccagcggg aagtcgtcta
     3721 ctggcatcac cggactaaaa catccctgtt tcaccgcggg cttgaacata gtatcgcctt
     3781 tgtccatgcg gacggcagca ttccggacag ctcacggttt aatgatgatg tgatcaccac
     3841 gtcattaatc tgtaccaacc gtatgatccc tgctcggctg catatcggcg atatctgcgt
     3901 ggccgtcaac aaaaacccgg cggtggcctc atttcataat gtcacacgcc caacgcagcc
     3961 gctctggcca gtcacggacg gcgacatgca ctggtcgctg atttccgcga tgaacctgaa
     4021 ctacctctcc ctgctggaca gggagacact gatccagatc ctgcggacct tcgacctgcc
     4081 aggcgctcac catccgcagc gggccagact gtcgcgccag aaactggatg ccatagaaaa
     4141 actcgaaacc aaacctgttg acaggttgtt taaaggcgtg ccggttcgcg ggctggcgac
     4201 cacgctgtgg atacgtcccg atccatttat ctgcgaaggt gaaatttatc ttcttggtac
     4261 agtgctctcg cacttttttg ctctctacgc cagcatcaat tcgtaccact gtctgaaaat
     4321 tatcaacacg gaaagtcagg aggcctggga atggcaggag aaaatgggtc agcacgccct
     4381 tatatagcgg taaaaccgcc gttatcggcg gatgtgcgca actgcaattt ctatgtgctg
     4441 atggaggcgc tgtaccgtcg gtatggcgct ccgggccagg atatttccct gcgtaccgag
     4501 ccagcccaag agatcgtgcg tttcagctct gacgccaata tcggttttcc gggtacggac
     4561 ctgagtgtgc tttcccgtag ccagaacgga cagtatgtac tgcaaacccg tttcctgggc
     4621 ttttccggca gccagtcgcc gctaccgggc tattatctgg atcagatggc gcaggagtcc
     4681 gcacagaacg aagacggcct gaaagaattt ctcgatctgt tcagccaccg ctggacgcag
     4741 tttgcctacc atgcctggcg caaataccgc tactacatct gctttcgtaa cggcggtaat
     4801 gacacctttt cacagcgcat gtacgcactg gtcggactgg gtaatcagag cgtgcgtgac
     4861 aggcttgcca ttaaccacag caaaatgctg gcctacgccg gaattctggc gacacctggc
     4921 cgcgcgccgg aggttatctg taatctggtg tcgcactgtt tcgatttgcc ggatgtcacc
     4981 attgaacgct ggcaactgcg taaagtcgcc attgatccgg cacaccagaa ccggctgggt
     5041 gtacgtaatc cgaaaggaaa aacggctgga catattccgg gacgctccat actcggcgta
     5101 aattttaccc tcggcgcacg ggtaccggat cgcagcggaa aattcctgtt gcagattggc
     5161 ggcctgtcgc gggagcgtta tctctcattt ttacccgatg gcgaaaatca cctgcctctg
     5221 accatgttcc tttcctttat tctgcgcgac cagtttgcct gggatttacg gctgtgcctg
     5281 gcaccacagc aggctaaagg gatgcgcctt ggcgatccgg ccagctcccg cctcggcaga
     5341 accagcttta ttggtcagcc gaaagtaccg cccgccatca ccatccgaat cagggaataa
     5401 ttctatggaa ccacttatgg ctgaggaaaa acagcaatcc cgccaggcct cactgacttt
     5461 acaggtgatg aatggcaacg aactggaaag cggacgcgcc gcaaaatgcc tgttcagcga
     5521 aaacggcggt gatattggtc atacgccgga atgtcactgg caggtacagg atcgggcggg
     5581 aagcattgct gcgcgtgcct gtaccgttat ccggcacgac ggagcctatt gcctgcggtg
     5641 cctgacgccg ggactgatga ttaatctggc cccggtttct tccgatgcgc tcatccgcct
     5701 gcgccaaggg gatgagatcc agctcggtgc actggcgttg aaagcgtttc tgcatgacgg
     5761 cgcggctgtc acctacgacg agcggatggc aaccccggaa acgattgtga tgaaccgcga
     5821 cagtctggcg gacaccctgc tgaccaccga gggacaaccg tcctatccgg gaatgccgtt
     5881 ccagcatcag cttgccccca cggtggcgca cggtttttcc agcgatccgc tccaggcact
     5941 ccagacggaa agcctgatgg tggcagatga tccgatcgca ccacgcgttt cccggcccgt
     6001 cgcccccgcc tccgaactga caggtgcaaa cgggatcaat acgccgttta tggatctgcc
     6061 gcccggcaat gcccgttatg aggacgacga tgaattttct gccatggcgc agtaccacct
     6121 ggccgtgacc ccgctgctgc gcggtatgga gtgcccgctg acgctgcacg attctcagga
     6181 tgcggatgag tttctcgaag aggccggacg ggcgttgcag gctgccatta agggattgct
     6241 gagcctgcaa caacaacaga acagcctgtc ggataaacac ctgcgtccac tggaagacaa
     6301 cccgcttcgc cttgatatgg actacgccac tgcgctgaac gtcatgttcg ctgagggtaa
     6361 aagtccggta catctcgcag cacccgcggc catcggcgaa agcctgcgca acatccggca
     6421 tcatgaagag gctaaccggg cggcgattgt ggaagcgttg cgtgtcatgc tggaggcctt
     6481 ctcgccgggc aacctgatgc gccgctttgc gcaataccgt cgcggccatg aactgcgcca
     6541 gaaaatggac gacgcctggg catggcagat gtacagcaac tattacgatg agctggcctc
     6601 cagccgacag caaggatttg aaatgctgtt caatgaagtt tatgcccagg tctatgaccg
     6661 ggtgctgcga gaaaaacagc gggagccgga agcatgaacg gcaaagcgtt tctggcctgc
     6721 gttctgatga gcgtcgtatt aactggctgt gaaacagcga aaaaaatcag ccaggtgatc
     6781 cgcaatccgg atattcaggt cggaaagctg atggatcagt caaccgagct aaccgtcacg
     6841 ctgctgaccg agccggacag caacctgacg gcggacggcg aagctgcgcc ggtggatgtc
     6901 caactggttt acctgagcga cgactccaaa ttccaggccg ccgactacga ccaggttgcc
     6961 accaccgcgc tgcccgacgt gctggggaaa aactatatcg atcatcagga cttcaacctg
     7021 ttgccggaca ccgtaaaaac actgccgccg atcaagctgg atgagaaaac cagttatatc
     7081 ggtgtcattg cctatttttc agacgaccag gccacagaat ggaaacaaat tgagccggta
     7141 gaaagtatcg gccaccacta tcgcctgctg gtgcacatcc gcgccagtgc gattgagatg
     7201 aaaaaagagg aaaactgacg atggcaacaa tgaaaaacaa ggtgatctgg caggaagggc
     7261 tgtttgccct gccgcagcat tttcagcaac agcagcgtca ctgcgagtac ctgctgaccc
     7321 gtcgtctgga tgcactgggt gattttttct ggggctttac ggaactctcc atcaataccg
     7381 aactgcttgc tcagggcaaa attatgattg accgtgcggc gggctgcatg cctgacggca
     7441 ccgtattcag tattcccgac caggatctgc tgccggaacc tttccagccg ggaacccttt
     7501 catcgaaaga gagccacagt atttatctgg cgctgccggt gataagcgac gtgatcaatg
     7561 aaatccaggg gttgcacagc gccggtcagg gtacggagcg ttaccgcctc acccataccc
     7621 gcgtccgtga ttttcacact gacgaaggcg acgaacagcc agtcggtctg tgacagttga
     7681 tccccaggat agtcagcggc gcagatgatc tcagcgcgat ggtgactctc cccctgtgcc
     7741 gtatcctcaa taaaaatgcc accggcgcgc tggtgctgga caacaccttt attcccacca
     7801 ttcaggccgt ccgtgtcagc gggttgctcg gggcattctc cggcgaggtg cagggactgc
     7861 ttgccacccg cgccgctgac ctggccgggc gcattggttc gcctgaacag agcgggatcg
     7921 ccgatgtagc cgaatttatg atgttgcaga tgcttaaccg ccatcaaatg cagttcacgc
     7981 atcgttccca actgcacacg ctgcatccgg aagccttcta ccgggatctg gtggggctgc
     8041 tgggagaact gatgaccttt accgaaggta atcgcctgcc ctgcaccgtg gagccatata
     8101 atcacaggga tcttaccgcg accttcacca gaaccgtgat cccggaactg cggcgcgcgc
     8161 tcaataccgt actggtgccg cgtgcgcaga atctgccgct gttcttttca gaggggacct
     8221 ggatcgccac catcaacgat cccacactgt tgcagtcctg caagctggta ctggcagtgc
     8281 gcgccaggat gccctacgac caggtacaac gccagttcat ccagcagtcg aaagtggccg
     8341 ctaccgacaa aatccgcagc gtggtcagcg tgcaggtgcc gggtattccg ctgcacacgc
     8401 ttaccgctgc gccgcgtcag ttgccctggc atgaaggtta tgtttacttc gaactggaaa
     8461 aaggcacccc agcctggcag gacgtgataa aagcgggcgc gctggcgctg catatctccg
     8521 gcaccttccc cgatctgaac cttcagctct gggcgataag gggataagtg aatgagcgat
     8581 atcagcgata tgggtgaacc gtcggttttt cccatggagc cggagagcgg aacatcagga
     8641 cgtcagtacc gtctcgccct tcgcggcaac agcctgaacc caatgattga cgccgccacg
     8701 ccgttgctcg gcatggtgat gcgtctgtcc accatgaaca gccaggccat gccagaacat
     8761 ctgtttgccc aggtggtaac cgatgtccag gccgtagaac aactactcca ggaacaggga
     8821 tacgaaccgg gcgtcattgt ttcgttccgc tacattctct gcacgtttat tgacgaagcg
     8881 gcgctgggca acggctggtc caacaaaaac gaatggatca aacagtcgct gctggttcat
     8941 tttcataacg aagcctgggg cggcgagaaa gtgtttatcc tgctcgaacg ccttatccgg
     9001 gagccgatac gttaccagga tctgctggaa tttttatacc tttgtttttc gctcggcttt
     9061 cgcgggcgct acaaggttgc cattcagcaa caggatgaat ttgagcggat ctaccagcgc
     9121 ctgcaccatg ccctgcacaa actgcgcggc gatgcgccat ttccgctgct tcaccagaag
     9181 aacaacatac agggtgggcg ttatcagtta atccggcggc tgaccatcag gcatgtaatg
     9241 tgcggaggtc ttgttgtgct ggccgtattt tatctgtttt atctgctgcg tctggacagc
     9301 cagacacagg atattctgca tcagctcaat aaattactcc gataaggaca tcttatgatc
     9361 cagattgatt tagccacgct ggtaaagcgg cttaacccct ttgcaaaaca ggcgctggaa
     9421 atggctgcct cggagtgtat gagccagcag gccagtgaga tcaccgttgc ccatgtgctg
     9481 ttacagatgc tggcaatccc gcgcaacgac gtaagggtga ttgccgaacg cacaggcatc
     9541 agtgcggagg atttacgcca ggcgctgacc gtggagagtt atcccggcgg acgttccgcc
     9601 gatggctatc ccagcttctc cccgatgctg atcgaatggc tcaaggagtc atggctgctg
     9661 gcctcagccc agatgcagca cagcgaactg cgcagcggcg tcctgctgct tactctgttg
     9721 cattcaccgc tgcgctatat tccacccgct gctgcccgtc tgctgacggt cataaaccgc
     9781 gaccagttac agcaggattt cgccgcatgg acaaaagaat ccgccgaatc cgtggatctg
     9841 gctggcgggc aaacgccccg cgcaacggaa accagcgaca ccctgctggc ccgctacgcc
     9901 aaaaacatga ccgcagacgc ccgtaacgga aaacttgacc cggtactgtg ccgcgaccac
     9961 gaaatcgacc tgatgatcga cattctctgc cgccgccgta aaaacaatcc ggtggtggtg
    10021 ggcgaagccg gcgtgggcaa aagcgcgctg attgaagggc tggcgctgcg catcgtggca
    10081 gggcaggtgc cggataagct gaaaaacacc gatatcatga ctcttgacct gggcgcattg
    10141 caggccggag catcggtgaa aggtgagttt gaaaaacgtt tcaaggggct gatggcggag
    10201 gtcatttcct ctccggtgcc ggtcattctg tttatcgacg aagcgcatac cctgattggc
    10261 gcgggcaacc agcagggcgg gctggatatc tccaacctgc tcaaaccggc actggcgcgt
    10321 ggcgagctga aaaccatcgc cgccaccacc tggagcgagt acaaaaaata cttcgagaaa
    10381 gacgccgccc tgtcgcgtcg cttccagttg gtgaaggtca gtgaacccag tgctgccgaa
    10441 gccaccatta ttctgcgcgg cctctcttcg gtctatgaac agtctcacgg tgtgctgatt
    10501 gatgatgacg ccttgcaggc cgctgcgaca ttaagtgagc gttatctctc cgggcgtcag
    10561 ttgccagaca aagccattga tgtgctggat acggcctgcg cacgtgtggc aatcaacctg
    10621 tcatcgccac cgaagcaaat ctcggcgctg accaccctga gccaccagca ggaggcggaa
    10681 attcgccagc ttgagcgcga gcttcgcatc gggctgcgta ccgacacttc acggatgact
    10741 gaggtgctgg tgcagtacga tgaaacgctg acggcgctgg atgaactgga agcggcctgg
    10801 caccagcagc agacgctggt acgggaaatt atcgcgctgc gccagctatt gctgggtgtg
    10861 acagaggaca cagatgccgt agtggatacg ccgccagagt cagaacagga taataccggt
    10921 accgcaccgg ctgatgatgc gggcagtaca cagccggaag agaccgctga aacagtttcc
    10981 ccggtgcagc agctagtaca actcactgcc gaactggacg ccctgcataa cgaccggttg
    11041 ctggtttccc cgcacgtcga taaaaaacag attgcggcgg tgattgccga atggaccggc
    11101 gtaccgctta accgcctgtc gcagaatgaa atgtcggtca tcaccgacct gccgaagtgg
    11161 cttggtgaca ccatcaaagg ccaggacctg gcaattgcca gcctgcataa acacctgctg
    11221 accgcacgcg ccgacctgcg ccgtccggga cgcccgctcg gcgcgttcct gctggctggc
    11281 cccagcggcg tgggtaaaac cgaaaccgtc ctgcaactgg cagagctgct ctacggcggt
    11341 cgccagtacc tgaccaccat caatatgtcc gagttccagg agaaacacac cgtctcgcgg
    11401 ctgattggct cccctccggg ctatgtcggc tatggcgaag gcggcgtact gaccgaagcg
    11461 atccgccaga aaccctactc ggtggtgctg ctcgatgaag tggaaaaagc gcacccggat
    11521 gtcctcaacc tgttctacca ggcgttcgac aagggcgaga tggcagacgg cgaaggccgc
    11581 ctgattgact gtaaaaacat cgttttcttc ctcacctcca accttggtta tcaggtgatt
    11641 gtcgaacacg ccgatgaccc ggaaaccatg caggaagtgc tctatccggt gctggcggac
    11701 ttctttaaac cggcgctgct ggcgcgtatg gaagtggtgc catacctgcc gctgtcgaaa
    11761 gagacgctcg ccaccattat tgccgggaaa ctggcccgcc tggataacgt gctatgcagt
    11821 cgctttggtg cggaagtgat tattgaaccg gaagtgacgg acgaaatcat gagccgcgtc
    11881 acccgcgcgg aaaacggcgc gaggatgctc gaatcggtca tcgatggcga catgctgccg
    11941 ccgctctcgc tgctgctgtt gcagaaaatg gcagccaaca cggcgattgc ccgtatccgc
    12001 ctgtcggcgg cagacggcgc gttcacggca gacgtggaag atgctcagga cgacgagttt
    12061 gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt tttccggcag gcaggatggt
    12121 ttatcgcggg cctgatggcg ggcttacccg ccagtgccgc gcccgcagaa acatcgtcaa
    12181 tggccggtgc ggcggtcgcc gtcgccacca cgcccccgcc ggatgcgaca gccacgttac
    12241 aggccatgca gtcctgtcgg cgggagtctg cggcgctgga gcggcttgac tgttatgacc
    12301 gcattcttgc accggaacag cccggcttcg gcaacgccgc actggtcaaa gccagctttg
    12361 tgggcgaagc ctggacccgc gccacagagc aggaaaaacg ccgccagggc aacaccacgg
    12421 agctgctggc gacgcaggtg ccgggggaac gcccgacggt agtgataacc accccggcta
    12481 tcggccatgt tccgccgcgc ccggtgttga tgttcagttg cgtggacaat atcacccgga
    12541 tgcaggtggc gctgatgcat cctctggatg ttcatgatat cgccgtcacc ctgaatgccg
    12601 acaaccgggc attgcgcagc cactggtttg tgcgcgagaa cggcaccctg ctggagtcca
    12661 gccgggggct gtccgggatt gatgaaatta agcaactgtt cggcgcaaaa acgctgacgg
    12721 tggataccgg cgcagataac gccgccggaa agctgacctt taacattgac ggattggcac
    12781 gggttattgc gccactacgc gacgcctgcc actgggcggg agaataagaa tatggcaatg
    12841 gacttacgta atcctgatgt atggcttgca cacctgctgg aaaacctgcc ggaagataag
    12901 ctgtccgccg cgcttgatga tggtaatgca gactgggagt ttatcgacag cgaaatcgtc
    12961 aagctcggct cactggcgca cagccagctt gatatccccg aacttcagcg ccggggcctg
    13021 atgcttctgg cctcagaaac caaagatttt cgcctgctgg cccacctgct gcgtaccctg
    13081 caacatgccg gtgatattct gctggcctcc aggatgctgg cacaatatac agagcactac
    13141 tggacatgcg ccgcaccgca gaacatggcc cacaaaaaac gctttgccgc ccaggtgata
    13201 aaacgctttg agtctgccgt tcaggacttt gccgggaatg ctgcgacaac acagcgcgat
    13261 gctttgctgg gagagctggc taaactggca cagtgctggc aggcgcacaa cgcaccggag
    13321 ttggctaagg cgacagacga tttatttgct ctgtttcagc gcgcgtttcg tgatgtagcg
    13381 cctgaaatgc catcatctgc acgaaccgcc gccagtgcgc cacaggccac gagcagatct
    13441 gtaccggata tgtcaccgca caccgctgcc gctcagcctc ctgttgcagc cactccaatc
    13501 ccgcaggtca tcgttgacag ccacgacgat aaagcatggc gcgacaccct gttgaaggtg
    13561 gccgccattc tctgtgaacg tcagcctgag tcaccgctgg gttaccgcct gcgccgccat
    13621 gcgctgtggc agaccatcac cagtaccccg caggcagaaa gcgatggccg gacccctctg
    13681 gctgctttcc ctgtcgatat gatggatgac tatctggcgc gactgaacaa tgccgacatg
    13741 gcgctttggc agcaggtgga aaaaagcctg ctgctggccc cgtactggct ggacggccat
    13801 tacctctcag cacagaccgc gttgcgcctt ggttatatac aggtggcgga tgctatccgt
    13861 gatgaagtta ccagttttct tgctcgcctg cccgcactga tcaacctgtt gtttaacgac
    13921 cgcactccgt ttgtgtccga acagacgaaa cagtggctgg caccatcggg ctcaggcaac
    13981 caaaccgcag cagctgtaca gaccgatgaa gaattacagg cagccagggc gtgcttcgat
    14041 gagaacgggc tggaaaccgc gctgcggtat ctggaaaacc tgcccgaagg tgatccccgt
    14101 aaccagtttc atcgccaatt tttcggcgcg cagttactgg aagaagccgg tatggttcag
    14161 cttgcacaac agcagtaccg gatgctcttc aaaacaggct tacacttgat gctacccgaa
    14221 tgggagcctt ccctgctgaa agcactggaa caaaaactca cggcagaaca gtaaaagata
    14281 acaaggagtt actgtgttca gatttccgac atcccgactg ttcagcacgc tgagatcagc
    14341 tctcaggcca gcgatgccgc ggtttaaagt ttccgccgcc tggctgctgg cgctggcatg
    14401 gatttttctg ctggtgtgga tctggtggca gggtccaaaa tggacgctct atgagcagca
    14461 ctggctggct ccgttggcaa accgctggct ggcgaccgcc gtctgggggc ttatcgctct
    14521 ggtctggctc acctggcggg tgatgaagcg tctgcaaaag ctggaaaaac agcagaaaca
    14581 gcagcgggat gaagaaaaag atccgttgac cgtggaacta catcgccagc agcaatatct
    14641 ggatcactgg ctgctgcgtc tgcgccgcca cctggataac cgccgttatc tgtggcagtt
    14701 accgtggtat atggtcattg gtcctgcggg gagcggcaaa agcacgctgc tgcgcgaggg
    14761 ctttccgtct gacattattt acacgccgga aagcatccgg ggtgtggaat accatccgct
    14821 gatcacaccg cgagtgggca accaggcggt aattttcgat gttgacggcg tactgacctc
    14881 ccccggaggg gatgatctgc tccaccgccg cctgcgcgaa cactggctgg gctggctgat
    14941 gcaaacgcgc gcacgccagc cgctcaacgg tctgatcctg acgctcgatc ttcccgatct
    15001 gctgaccgcg gataaacccc gtcgcgaagc actggtacaa aatctgcgtc agcaacttca
    15061 ggaaattcgc cagtgcctgc actgccggtt gcccgtttat gtggtgctga cccggctgga
    15121 tctactgacc ggttttgccg cgctgttcca ttcgctggat aaaaaagacc gcgatgcgat
    15181 cctcggcgtc acgtttaccc gccgcgctca tgaaaacgat gactggcgca gcgagctgag
    15241 caccttctgg cagacctggg gacaacagat gaacctggcc ctgtcagacc tgatgctcgt
    15301 gcagaccggt gcagcactgc gcagcgccgt gttcagcttc tcccgtcaga tgcagggaac
    15361 gggagaaatc gtcaccgcac tgctcgccgc attgctggac ggtgagaaca tggacgtgat
    15421 gctgcgcggc gtctggctca catcctcgct acagcgcggc caggtggatg atattttcac
    15481 gcagtctgcc gcccgccagt acgggctggg caacagctcg ctggcaacct ggcctctggt
    15541 ggagacgacg ccctatttca cccgccgcct cttccctgaa gtcctgctgg ctgagccgaa
    15601 cctggcgggt gaaaacagcg tctggctgag cagctcccgg cgcaggctga ccgccttttc
    15661 cgcctgtggc gcggcgctgg cggcattgct ggtcggaagc tggcaccatt attacaacca
    15721 gaactggcag tccggcgtta acgtactggc gcaggctaaa gcctttatgg acgtaccacc
    15781 accgcaggga atggatgaat ttggcaatct gcaactgccg ttgcttaatc cggtacgcga
    15841 tgccaccctg gcctatggcg attaccgcga tcacggtttt ctggcggata tgggattgta
    15901 ccagggcgtc cgcgtagggc cgtatgtgga gcaaacctac attcagcttc ttgagcagcg
    15961 ttatctcccc tcattaatga acggcctgat ccgggatctg aacaatgccc cgccagagag
    16021 cgaagaaaag ctcgccgtgc tgcgcgtact gcgcatgatg gaagacaaaa gtgggcgcaa
    16081 caacgaggcg gtaaaacagt acatggcgcg gcgctggagc aatgaatttc acggccagcg
    16141 tgatattcag gcgcaactga tggcgcatct ggactatgcg ctcgagcaca ccgactggca
    16201 cgcgcagcgc cagagcggcg acagcgatgc tgtcagccgc tggacaccct atgataaacc
    16261 ggtcattaat gcgcaacatg aactgagcaa gctgcccata taccagcgtg tctaccagac
    16321 cctgcgcacc aaagcattaa gcgtgttgcc cgccgatttg aatttgcgcg accaggttgg
    16381 tcccaccttc gacaacgtgt tcgtcgccgg taatgatgaa aaactggtga ttccgcagtt
    16441 tctcacccgc tatggcctgc aaagctattt tgtcaaacaa catgacggcc tcgttgagct
    16501 gaccgcgctg gattcgtggg tgctgagcct gacgcaaagc gtcgcctaca gcgaggccga
    16561 ccgtgaagag atccagcgcc atatcactga acagtacctc agtgactata ccgccacctg
    16621 gcgtgccgga atggataacc tcaacgtccg tgactatgag gccatgccgg agctgaccga
    16681 cgcgctggag cagattatca gcggcgatca gccattccag cgtgcgctga cggcgctgcg
    16741 cgataatacc cacgcgctga cgctctccgg caaactggac gataaggcgc gggaagcggc
    16801 gataaatgag atggattacc gcctgttgtc ccggctgggg catgagttcg cgccggaaaa
    16861 cagcgcactg gaggagcaaa aggacaaggc cagtacgcta caggccgtgt accagcaact
    16921 gaccgagctg caccgttacc tgctggcgat ccagaactcg ccagtgccgg ggaaatcggc
    16981 gctgaaagca gtacagctac gtctggatca aaacagcagc gatccaatct tcgccacccg
    17041 ccagatggca aaaaccctgc ctgcgccgct taaccgctgg gtgggcaagc tggcggatca
    17101 ggcctggcat gtggtgatgg tggaagccgt tcattacatg gaagtggact ggcgcgacaa
    17161 tgtggtgaaa cccttcaacg aacagcttgc cggtaactat ccgtttaatc cacgcgcccc
    17221 acaggatgcc tcactggatg cgtttgagcg cttctttaaa ccggatggca ttctggacac
    17281 gttctacagg gacaacctgc gactgttcct ggaaaacgat ctgacctttg gcgacgacgg
    17341 cagagtgctg atccgtgaag atatccggca acaactggat accgcacaga aaatccgcga
    17401 catcttcttt agccagcaga acggactggg cgcacagttt gccgtggaaa ccgtatcgct
    17461 ttccggcaat aagcggcgca gcgtacttaa cctggacggc cagttagtgg actacagcca
    17521 gggacgcaac tacaccgccc atctggtctg gccgaacaac atgcgtgaag gcaatgaaag
    17581 caagctgacg ctgattggca ccagcggcgg agcaccgcgc agtatcgcgt tcagtggacc
    17641 gtgggcgcag ttccgcctgt tcggcgcggg ccagttgacc aacgtaacca gtgacacctt
    17701 taacgtgcgc tttaacgtgg acggcggcgc gatggtttac cgggtgcatg tggataccga
    17761 agataacccg ttcaccggcg gtctgttcag ccagttccgt ttaccggata cattgtatta
    17821 aggggatagt gatgaacaac aacgcactga ctcaaactat cgttaccggc agcgatccgc
    17881 gcgccctgcc ggagttcagc gccatccgcg aagaaattaa caaggctaat cacccgtcac
    17941 agccggagct gaactggaaa ctggtggaat cgctggcgct gtcgattttt aaggccaacg
    18001 gggtggattt gcataccgcc acctactata cactggcgcg tacccgtaat cagggactgg
    18061 cagggttctg cgaaggcgcg gaactgctgg cagcgatgat caaccacgaa tgggataaat
    18121 tctggccgca gggtggcccg gcgcggactg agatgctgga ctggttcaat acccgcaccg
    18181 ggaatatcct gcgccagcag gtgtcctttt ccgaaaatga tctgccgttg ctctaccgca
    18241 cggaacgggc tttacagctt atctgtgaca aactccagca ggtggaactg aagcgccagc
    18301 cgcgcgtgga aaacctgctt tattttgtgc agaacacacg caagcggttt gaaccgcagc
    18361 ccggaaaccg tactgatacg gcggcacaga ccacggtccg gacgctggtc tatgcgccgg
    18421 aaagcacggc ttccaccaca gcggaaacca tcccccccct acccgacctg ccggaaatgc
    18481 gggtggaggt gcgcggagtg gcagataacg ccgacaaagc aaaacagggc gacacggtaa
    18541 aaggttttgt tgccggtgcc gcctgtactg ccgttatcgc tgctgcactc tggtggtggc
    18601 agacgtaccc gatgcagcgg cagcttacgc aggtcaggga taccgcacag ggagccgcca
    18661 ccgtctggct ggcatcaccg gtactgaaag aatatgaaca gaacctgcaa cagcttctga
    18721 ataccccacc gctacagccg ctggaaaccg gaatgcagat gatgcgcacc gcagacaccc
    18781 tctggccgga aagcctgcaa cagcaggaag ccagccggat gtggagcacc acgctgagaa
    18841 accgggcgca agccagtccg cagatgaagg gctggcaaca ggcgcggcag aatttgcggg
    18901 attttgcgga tctgatgatg aaaaaggaga cggagaaaca gggtttcacc ctctcgtata
    18961 tcaaaacggt gacctggcag gccgaacggt tactgaatca ggaaactcca ctggagtaca
    19021 tgctgacgca gtaccaggag acccgcacgc agaatcagga cacacaggcg ctagcaaaag
    19081 agatcaacca acggctggat gggctgttaa gccgctggct gttgctgaaa aataccggac
    19141 aggatatggc aacggacaac agcaccgtgc cagtccctcc gacccattga gtttaaggag
    19201 aataacaatg gcaaatcccg tctatctgac actaaacgga gaactccagg ggctgatttc
    19261 atccggatgc tcgtcaatgc cgtccatcgg taacaaagcc cagattgcac atcaggatca
    19321 gatcatggtg acgtcactgt cccatggtct gagccgggcg cagaatgtca accaccagga
    19381 gctgaccatc acgaaaccgg tggacaaatc ttcccccctg ctgggcaagg ccatttcgga
    19441 gaatgaatgt ctgacctgtg attttgtctt ctaccgcacc aaccgctttg gtattaatga
    19501 accgtactac aagctgaaac tgactaatgc ccggatctca aacatcggcc ttaccgttcc
    19561 ccataccatt aacgacagcc caggacagcc ggaagagtct gtgtcattta cctatgagag
    19621 tatcaactgg gagcacagcg tcgccggaac cagtgcgtac agcctttgga gtgaacggat
    19681 tttctaagtt acctgggagt ttttcatctg cattattgtt atattttaaa aaggcaattt
    19741 aaca
//
LOCUS       DAZFFG010000087        19364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFFG010000087 DAZFFG010000000
VERSION     DAZFFG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.087"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(135..1784)
                     /gene="pgi"
                     /locus_tag="ACHJ7J_003490"
     CDS             complement(135..1784)
                     /gene="pgi"
                     /locus_tag="ACHJ7J_003490"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO5001675.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSQAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2021..2217
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2309..3658
                     /gene="lysC"
                     /locus_tag="ACHJ7J_003491"
     CDS             2309..3658
                     /gene="lysC"
                     /locus_tag="ACHJ7J_003491"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO5001676.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTGNPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3911..4183
                     /locus_tag="ACHJ7J_003492"
     CDS             3911..4183
                     /locus_tag="ACHJ7J_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO5001677.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4184..5056)
                     /gene="rluF"
                     /locus_tag="ACHJ7J_003493"
     CDS             complement(4184..5056)
                     /gene="rluF"
                     /locus_tag="ACHJ7J_003493"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO5001678.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5404..6351
                     /locus_tag="ACHJ7J_003494"
     CDS             5404..6351
                     /locus_tag="ACHJ7J_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5001679.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6421..7227
                     /locus_tag="ACHJ7J_003495"
     CDS             6421..7227
                     /locus_tag="ACHJ7J_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5001680.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7237..7644
                     /locus_tag="ACHJ7J_003496"
     CDS             7237..7644
                     /locus_tag="ACHJ7J_003496"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO5001681.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNTNVEAVEFVPGENAGDIV
                     AKLKKLVSIHSQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQSCVVWKQLATTEEDF"
     gene            7644..8138
                     /locus_tag="ACHJ7J_003497"
     CDS             7644..8138
                     /locus_tag="ACHJ7J_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5001682.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAIAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8204..9001
                     /locus_tag="ACHJ7J_003498"
     CDS             8204..9001
                     /locus_tag="ACHJ7J_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO5001683.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9012..9836
                     /locus_tag="ACHJ7J_003499"
     CDS             9012..9836
                     /locus_tag="ACHJ7J_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO5001684.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9888..11117
                     /gene="sorE"
                     /locus_tag="ACHJ7J_003500"
     CDS             9888..11117
                     /gene="sorE"
                     /locus_tag="ACHJ7J_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO5001685.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYEGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILTRHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11498..12187
                     /gene="pepE"
                     /locus_tag="ACHJ7J_003501"
     CDS             11498..12187
                     /gene="pepE"
                     /locus_tag="ACHJ7J_003501"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO5001686.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12277..13908)
                     /locus_tag="ACHJ7J_003502"
     CDS             complement(12277..13908)
                     /locus_tag="ACHJ7J_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO5001687.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14128..17811)
                     /gene="metH"
                     /locus_tag="ACHJ7J_003503"
     CDS             complement(14128..17811)
                     /gene="metH"
                     /locus_tag="ACHJ7J_003503"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO5001688.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18011..18835
                     /gene="iclR"
                     /locus_tag="ACHJ7J_003504"
     CDS             18011..18835
                     /gene="iclR"
                     /locus_tag="ACHJ7J_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO5001689.1"
                     /translation="MVAPIPAKRGRKPAAATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            19152..>19364
                     /locus_tag="ACHJ7J_003505"
     CDS             19152..>19364
                     /locus_tag="ACHJ7J_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001690.1"
                     /translation="MITRIPRSSFSVNINNTAQTNEHQNLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIK"
BASE COUNT         4608 a         5033 c         5043 g         4680 t
ORIGIN      
        1 ccgaatacga tgtacatatc ggcatcgacc tgtaggcctg ataagacgcg accgcgtcgc
       61 atcaggcatc ggttgccgga tgcggcgtga acgccttatc cggcctacat agcggcatca
      121 ggcatgaacg atgattaacc gcgccacgct ttatagcggt taatcagacc attggtcgag
      181 ctatcgtggc tgctgatttc tttatcatct ttcagctctg gcagaatacg gttcgccagc
      241 tgtttaccca gttccacgcc ccactggtcg aaggtgaaga tgttcaggat cacgccctga
      301 gtaaagattt tgtgctcata cagcgcaatc aacgcaccca ggctgaacgg agtgatttca
      361 cgcagcagga tggagttggt cgggcggtta ccttcgaaca ctttgaacgg caccacgtag
      421 tcaagcgttg ccggatcttt accctgatcg cgatattcct gctcaaccac ttcgcgggat
      481 ttaccaaacg ccagcgcttc ggtctgagca aagaagttag acagcagttt ctggtgatga
      541 tcagagagcg ggttatgggt gatagccgga gcgatgaaat cgcacggtac cattttagtt
      601 ccctggtgga tcagctggta gaacgcgtgc tgaccgttag tgcctggttc accccagata
      661 atcgggccag tctggtaatc cacaacgtta ccgttacggt caacatactt accgttggac
      721 tccatattgc cctgctggaa gtacgccgcg aaacggtgca tatactggtc atacggcaga
      781 atcgcttcag tttccgcacc aaagaaattg ttgtaccaga tgccaatcag cgccagcagg
      841 acaggcaggt ttttctcggc aggcgtggtg gagaaatgct tgtccatcgc gtgtgcgcca
      901 gaaagcagtt caacgaagtt atcaaagccg atggagagaa caatcgacag gccaatcgct
      961 gaccacaaag agtaacggcc gccgacccag tcccagaact cgaacatgtt ggcagtatca
     1021 ataccaaact cgccaacggc tttggcattg gttgagagcg ccgcaaagtg tttcgcaacg
     1081 tgtttttcat cacctgccgc tttcaggaac cagtcacgcg cgctatgggc gttggtcatg
     1141 gtttcctgag tggtgaaggt tttagatgct accaggaaca gcgtggtttc cgggtttact
     1201 tttttcagca cttcagcgat gtgagtccca tcgacgttag aaacaaagtg catgttcagg
     1261 tgatttttgt acggacgcag tgcttcggtc accatgtacg ggccgaggtc agaaccgccg
     1321 atcccgatgt tcactacgtc agtgattgct ttgccggtat aacccttcca ctcaccggag
     1381 ataatcgctt gtgagaaggt tttcatcttc tccagcaccg cgttgacttc cggcattacg
     1441 tctttgccat caaccagaat cggggtattg ctacggttac gcagcgctac gtgcagcaca
     1501 gcgcggtttt cagtgcggtt gatcttctcg ccagagaaca tcgacttaat cgcgcccgcc
     1561 agatcgcact ctttcgccag atcttgtaat ttcgccagcg tctcttcagt gatgcggttt
     1621 ttggagtaat ccaccagcat ctgatcgtcg aaggttgcgg agaacttaga aaaacgatcg
     1681 ccgtctttag caaaaagatc ggcgatcgta acgtctttca tttcatcgaa gtgtttctgt
     1741 agtgcctgcc aggcagcggt ctgcgttgga ttgatgtttt tcattagcaa tactcttctg
     1801 attttgagaa ttgtgacttt ggaagattgt agcgccagtc acagaaaaat gtgatggttt
     1861 tagtgccgtt agcgtaatgt tgagtgtaaa cccttagcgc agtgaagcat ttattagctg
     1921 aactactgac cgccaggagt ggatgaaaaa tccgcatgac cccgtcgttg acaaccgccc
     1981 cgctcaccct ttatttataa atgtactacc tgcgctagcg caggccagaa gaggcgcgtt
     2041 gcccaagtaa cggtgttgga ggagccagtc ctgtgataac acctgagggg gtgcatcgcc
     2101 gaggtgattg aacggctggc cacgttcatc atcggctaca ggggctgaat cccctgggtt
     2161 gtcaccagaa gcgttcgcag tcgggcgttt cgcaagtggt ggagcacttc tgggtgaaaa
     2221 tagtagcgaa gtatcgctct gcgcccaccc gtcttccgct cttcccttgt gccaaggctg
     2281 aaaatggatc ccctgacacg aggtagttat gtctgaaatt gttgtctcca aatttggcgg
     2341 taccagcgta gctgattttg acgccatgaa ccgcagcgct gatattgtgc tttctgatgc
     2401 caacgtacgt ttagttgtcc tctcggcttc tgctggtatc actaatctgc tggtcgcttt
     2461 agctgaagga ctggaacctg gcgagcgatt cgaaaaactc gacgctatcc gcaacatcca
     2521 gtttgccatt ctggaacgtc tgcgttaccc gaacgttatc cgtgaagaga ttgaacgtct
     2581 gctggagaac attactgttc tggcagaagc ggcggcgctg gcaacatctc cggcgctgac
     2641 agatgagctg gtcagccacg gcgagctgat gtcgaccctg ctgtttgttg agatcctgcg
     2701 cgaacgcgat gttcaggcac agtggtttga tgtacgtaaa gtgatgcgta ccaacgaccg
     2761 atttggtcgt gcagagccag atgtagccgc gctggcggaa ctggccgcgc tgcagctgct
     2821 cccacgtctc aatgaaggct tagtgatcac ccagggattt atcggtagcg aaaataaagg
     2881 tcgtacaacg acgcttggcc gtggaggcag cgattatacg gcagccttgc tggcggaggc
     2941 tttacacgca tctcgtgttg atatctggac cgacgtcccg ggcatctaca ccaccgatcc
     3001 acgcgtagtt tccgcagcaa aacgcattga tgaaatcgcg tttgccgaag cggcagagat
     3061 ggcaactttt ggtgcaaaag tactgcatcc ggcaacgttg ctacccgcag tacgcagcga
     3121 tatcccggtc tttgtcggct ccagcaaaga cccacgcgca ggtggtacgc tggtgtgcaa
     3181 taaaactgga aatccgccgc tgttccgcgc gctggcgctt cgtcgcaatc agactctgct
     3241 cactttgcac agcctgaata tgctgcattc tcgcggtttc ctcgcggaag ttttcggcat
     3301 cctcgcgcgg cataatattt cggtagactt aatcaccacg tcagaagtga gcgtggcatt
     3361 aacccttgat accaccggtt caacctccac tggcgatacg ttgctgacgc aatctctgct
     3421 gatggagctt tccgcactgt gccgggtgga agtagaagaa ggtctggcgc tggtcgcgtt
     3481 gattggcaat gacctgtcaa aagcctgcgg cgttggcaaa gaggtattcg gcgtactgga
     3541 accgttcaac attcgcatga tttgttatgg cgcatccagc cataacctgt gcttcctggt
     3601 gcccggcgaa gatgccgagc aggtggtgca gaagctgcat tttaatttgt ttgagtaaat
     3661 actgtatggc ctggaagcta tatttcgggc cgtattgatt ttcttgtcac tatgctcatc
     3721 aataaacgag cctgtactct gttaaccagc gtctttatcg gagaataatt gcctttaatt
     3781 tttttatctg catctctaat taattatcga aagagataaa tagttaagag aaggcaaaat
     3841 gaatattatc agttctgctc gcaaacgaat tcccgtacac tgaactctct ggctcactga
     3901 gggcaccatc atggcactcc cccgtattac ccaaaaagag atgactgaac gcgagcagcg
     3961 tgaacttaaa acactgctgg atcgggcgcg gattgcgcat ggtcgcgtac tgaccaactc
     4021 cgaaaccaac agcattaaga aagagtacat cgataaatta atggtcgagc gtgaggctga
     4081 agcgaaaaaa gcccgccagt taaagaaaaa gcaggcttat aaaccggatc ctgaagcgtc
     4141 atttagctgg tcagcaaata cgtcgacgcg cggcaggcgc tgattagcgc cccttctttt
     4201 tgcgccccgg cgaggtaaaa cgcttaccgt tggacgccgg gcgaccgcct ttttccgccg
     4261 ttttttccat cttaacgact ggacgtttga tgcccgccgt tttcggttta gccttcgctt
     4321 taggttttgc ctcggaagag gaattttcga tgagtttaaa gaggtcgatt aactcatcgt
     4381 cggttaaatc gcgccactcc cccagcggaa tgccgcttaa gctgacgttc ataatgcgcg
     4441 tacgttccag ctttttcact tcgtagccga aatgctcgca catgcgccgg atctgacggt
     4501 tcagcccctg caccagggta atgcggaaga caaacggcgc ttcttttttg actttgcact
     4561 ttttagtcac cgtcccgagg atcggcaccc ccgcccccat accacgaata aattcatcgg
     4621 taatcggttt atcgaccgtc accagatact ctttctcatg atcgttaccc gcacggagga
     4681 tcttattcac cagatcgccg tgattggtga ggaaaatcag cccctgggag tctttatcca
     4741 ggcggccaat cgggaacacg cgtttgctgt ggttaacgaa atcgacaatg ttatcgcgct
     4801 cgccatcttc ggtggtgctg acaataccga cgggcttgtt cagggcgata agtaccaaat
     4861 cttcggcttc ccgaggttca atcaactgac cgtttacttt cacaacgtcg ccgggtttca
     4921 cctgatcgcc aatggtggct cgcttgccat taaggaacac attgccttgc tcgatatagc
     4981 gatccgcttc gcggcgtgag caaattccgc tttcgctgat gtatttattt aaacggactg
     5041 atgagtcggg cagcataaat tctcctgtaa aacgcggaat ataccgtacc tcttagcggg
     5101 gaaaaacagg gttatgcggc aacgtcgaaa ttaaatgtga tctgccacgc cttttatcac
     5161 aaaagtgtct ccggaagtac aatgcaaatt tgcctggcaa aataaggata atcaacatat
     5221 tacgaatcgc tgcacacctg tgctgatgac tactgcgcag agaaacaaat gtgcaagaga
     5281 aagcgctatt tctccccatg caaagagtga aaaatcgctt tgaaaggcca tcatgatggg
     5341 gttatatcag atcaagcggt gagaaagcgt atttgcacaa atgtgcagaa ccaggaggcg
     5401 ggaatggaaa acagtgacga tatccgtttg attgtgaaga ttgcccaact ctattacgaa
     5461 caggatatga cgcaggcgca aatcgcgcgc gaactgggta tttaccgcac caccatcagc
     5521 cgcttgctta aacgaggccg cgatcaggga attgtcacca tcgccatcaa ctatgactac
     5581 aacgaaaatc tctggctgga gcagcaactg aagcaaaagt ttggcctgaa agacgttgtg
     5641 gtggtgtcgg gaaatgatga ggatgaagag actcaactgg cgatgatggg gttacacggc
     5701 gcgcaactgc tggatcgctt gctggagcct ggcgatattg tcggtttttc ctggggtcgc
     5761 gcggtgagcg cactggttga aaacttgccg caggcggggc aatcgcggca gttaatctgc
     5821 gtaccaatta ttggcggacc gtccggtaaa ctcgaaagcc gctatcacgt aaacacatta
     5881 acctacagcg cggcagcgaa gctgaaaggg gaatcgcatc tcgcggattt tccggctcta
     5941 ctggataacc cattaattcg taatgggatc atgcagtctc agcactttaa aaccatctct
     6001 gcctactggg ataatctgga tgtcgccctg gtgggaattg gctcaccggc cattcgcgac
     6061 ggcgctaact ggcatgcgtt ttatggtggt gaagagagtg acgacctgaa tgcccgccag
     6121 gttgctggcg atatttgctc gcgctttttt gatattcacg gcgcaatggt tgaaacgaat
     6181 atgagcgaaa aaacactctc tatcgaaatg aataaattaa agcaggcacg atattccatt
     6241 ggcattgcca tgagtgaaga aaaatacagc ggaattattg gtgcactgcg tggaaaatat
     6301 attaattgtc tggtaacgaa tagcagcaca gctgaactat tactgaaata aaaatcttcc
     6361 cgatgtttat cgtaataaaa attacggcgg gtccctatta tctaaaatca ggagtctgtt
     6421 atgcaaacgt ggttaaattt gcaggataaa atcattatcg tcaccggcgg cgcatccggt
     6481 attggtctgg cgattgtaga ggaattatta gcacaaggcg caaacgtaca gatggtagat
     6541 attcacggtg gcgatggtca atatgaagtt cataaaggct atcagttttg gccgaccgat
     6601 atttccagcg ccaaagaggt aaatcatacg gtagcggaaa ttatccagcg ttttggtcgc
     6661 atcgacggtc tggtcaataa cgccggggtc aatttcccgc gtctgctggt cgatgagaaa
     6721 gcgcctgccg ggcagtatga actcaacgaa gctgcattcg aaaaaatggt caatatcaac
     6781 cagaaaggcg tttttctgat gtcgcaggcg gtggcgcgac agatggtcaa acaacatgat
     6841 ggcgtgattg tgaatgtttc ctcagaaagt ggactggaag gctcagaagg ccaaagctgt
     6901 tacgccgcta ccaaagccgc gctcaatagc ttcacgcgct cctggtcgaa agagctgggt
     6961 aaacacggta tccgtgtggt cggtatcgcg cccgggattc tggaaaaaac cgggctacgc
     7021 acgccggaat atgaagaggc gctggcgtgg acgcgcaata tcaccgtcga acagctgcgt
     7081 gaaggctata ccaaaaacgc cattcctatt gggcgcgccg gaagattagc agaagtcgct
     7141 gattttgttt gttatctgtt gtctgaacgc gccagctaca tcaccggagt aaccactaac
     7201 attgcaggcg gcaaaacgcg cggataagga ggctttatgg tcaacgccat tttttgtgcc
     7261 cacggcaaat tagcttgcgc catgctggaa tcggttcaga tggtttacgg caacaccaat
     7321 gttgaagccg ttgaatttgt gcccggtgaa aacgccggag acatcgtagc aaagctgaaa
     7381 aagttagtaa gcattcacag tcaagatgag tggctgattg ccgtggattt acagtgcggc
     7441 agcccgtgga atgcggcagc gatgcttgcg atgcgcaatc ctcgtctgcg ggtaatcagc
     7501 ggactttctc tgccgctggc gctcgaactg gttgataacc aggacagcat gaatgttgat
     7561 gaactgtgtg aacacctgac gcaaatcgca aagcaatcct gcgttgtctg gaaacaactg
     7621 gcaacgacag aggaggattt ctgatgaaca ttacgctcgc ccgcattgat gaccgcctga
     7681 tccacggtca ggtcactacc gtctggtcga aagtggctaa cgcccagcgc attattatct
     7741 gcaatgacga agtttataac gatgaagttc gccggacatt attgcgtcag gctgcgccac
     7801 cgggaatgaa ggttaacgtg gtcaatattg aaaaagccat cgcggtttat cacaacccgc
     7861 aatatcagga cgaaaccgtt ttttatcttt tcactcgccc acaggatgcc ttagcgatgg
     7921 ttcgccaggg ggtaaaaata ggcaccctaa atattggcgg catggcctgg cggcccggta
     7981 aaaaacagtt aaccaaagcg gtttcattag atgatgatga tattaacgca tttcatgagt
     8041 taaataatct tggcgtcata ctggatttac gcgtcgtagc gtcagatccc tcaatcaata
     8101 ttatcgacaa aataaacgaa cagttaatcg caaattaaat aatagcgcct gtgtacatat
     8161 gctttaacag gcagggatga cgacacttaa aaggtgacaa atcatggaaa taagtaccct
     8221 acaaattatt gctatatttc ttttttcctg tattgccgga atgggcagcg tgctggatga
     8281 atttcagact catcgcccgt taattgcctg tacggtgatt ggtttaattc tcggtgattt
     8341 aaaaaccgga ataatactcg gtggtacgct ggaattgata gctctcggct ggatgaacgt
     8401 cggcgcggcg caatctccgg actctgcact cgccagcata atctccgcca ttctggttat
     8461 cgttggtcag caaagcatcg ccaccggaat cgccatcgcg ttgcctgtgg ctgcggcagg
     8521 ccaggtgctg acggtgtttg cccgtaccat caccgtagtg ttccagcacg cggcggataa
     8581 agcagcggaa gaggcgcggt ttcgcactct cgatattctg catgtctccg cgcttggcgt
     8641 gcaggcgctg cgcgttgcta ttccggcact gattgtctcg ctgttcgtca gcgccgatat
     8701 ggtcagcaat atgttaaacg ccattcccga atttgtgacc cgaggactac agattgctgg
     8761 cggttttatc gtggtggtcg gttacgccat ggtgctacgt atgatgggcg tgaaatattt
     8821 gatgcctttc tttttcctcg gtttcctcgc aggtggctac ctcgatctca gtctgctggc
     8881 gttcggtggc gtcggcgtga tcatggccct ggtctacatc cagctaaatc cacagtggcg
     8941 taaagctgaa cctcaactcc aggccaccgc ctccaccgcc cttgaccaac ttgacgatta
     9001 acggagccat catggaacag agaaaaatta cacgcagcga tctggtgagc atgtttctgc
     9061 gctccaacct gcaacaggcg tcctttaact ttgaacgtat tcacgggctg ggtttttgct
     9121 acgacatgat ccccgccatc aagcgacttt acccattaaa agaggatcag gttgcggcgc
     9181 tcaggcgaca cctggtgttc ttcaatacca cgccagccgt atgtggcccg gtcatcggcg
     9241 tcactgccgc catggaagag gcgcgggcca acggcgcgga aattgatgac ggtaccatta
     9301 acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca ctggtctggg
     9361 gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg ggcaacattc
     9421 tcggcccgct gctgttcttc tttattttca atgcggtgcg tctggcgatg aagtggtatg
     9481 gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc gggaatgtgc
     9541 tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc gtgctggtca
     9601 ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc gccgatggct
     9661 ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt ttgctggcgc
     9721 tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg gtatggctga
     9781 ttttcgccct gtttggctta gggattatcg gcaatgcgct gggcttcctg tcctgattct
     9841 tcgccccggc acgactgccg gggccatcgc tcaacataag gtggtttatg aaaacaacag
     9901 ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgatctt cctgaaatgc
     9961 aggaggatga aatcctcgcg acggtggtca ctgacagcct gtgcctctct tcctggaaag
    10021 aggccaacct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc aacccaatca
    10081 tcatcggcca cgagttttgc ggcgatattc tggccgtggg taaaaagtgg cagcacaaat
    10141 tccagccggg tcaacgttat gtaatacagg ccaacctgca actccccgac cgcccggact
    10201 gccccggcta ctccttcccg tgggtgggtg gtgaagccac acatgtggtt atcccaaatg
    10261 aggttatgga acaagattgc ctgctggcat acgaaggcga aacctacttt gaaggctcgc
    10321 tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat cttcaggaag
    10381 gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc ctcggcggca
    10441 ccggaccaat gggactgttg gcgattgatt atgcgctaca tggacccgtt aacccgtcgc
    10501 tgctcgtcat taccgatacc gacaacgata aattgagtta tgcgcgcaag cactatccat
    10561 cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt gatacgctaa
    10621 tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct aatgaaggac
    10681 tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc ttcgccggac
    10741 cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat gcatttaccc
    10801 actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcagcggtc aaattgattg
    10861 aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg aatgccgcgg
    10921 gcgaaaccac gcttgaatta cctgccgtcg gcggcggcaa aaagctggtg tataccggga
    10981 aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg gcgattctga
    11041 cgcgtcatca ggggatctgg tccggtgagg cggagcaata tctgctcact catgcagagg
    11101 caatttccca tgattaatcg cgttactcaa ctctgcatgt cgctggctgg tcgcaccagc
    11161 aacttcgcga ccggatgtgc tgctgattgc tgaagcagca caatttgcac gagaagcata
    11221 aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtgcacat tgtttatgcc
    11281 ggatgcggaa tgaagcctta ttcggtctac ataattttga aaattcaata tattgcagtt
    11341 atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca tcagtctcag
    11401 cgcctgtgca atacgtgaca tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta
    11461 gcattccttc cgtgatttct tactaaccgg agaagtaatg gaactgcttt tattgagtaa
    11521 ctcgacgctg ccgggtaaag cctggctgga acatgcactg ccgctaattg ctgaacagtt
    11581 gcagggtcgc cgctcagcgg tgtttatccc tttcgctggc gtaacgcaga cctgggatga
    11641 ttacacagcg aaaacggctg cggttcttgc tccgctgggt gtttctgtca ccggtattca
    11701 tagcgttgtc gatcccgttg ccgcgattga aaatgctgag atcgtgattg tcggcggcgg
    11761 gaatactttc cagttgctga aacagtgccg cgagcgcggg ctgctggcac caattactga
    11821 cgtggttaaa cgtggtgcgc tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc
    11881 aaccattcgt accaccaacg atatgccgat tgtcgacccg caaggtttcg atgcgctgaa
    11941 tctgttcccg ttgcaaatca acccgcactt caccaacgcg ttgccggaag gccataaagg
    12001 tgaaacccgt gaacagcgta ttcgcgaact gctggtcgtc gcgccagaac tgacgattat
    12061 tggtctaccg gaaggtaact ggatcacagt gagtaaaggt cacgcaacgc tgggtggccc
    12121 gaacaccacc tatgtgttta aggccggtga agaagcggtt ccgctggaag cgggtcaccg
    12181 tttttaagtg aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc tgatgcgacg
    12241 ctttacgcgt cttatcaggc ctacagggtc acggtattaa tactcgtccc gtccttcatc
    12301 ttcatccggc tgttccagca cactgtaagc caccgaacaa aacagcgagt tcagacgctg
    12361 catatctccc agtaagccta aatgtagcga actggtttca atgctctgca cgttttgctg
    12421 atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa aacgatgttt
    12481 gctgcggcgc aaacgacggg cgctggtaac atcgccagag aagaaaaccg acattgccag
    12541 ttttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaagc catcaagcga
    12601 aaatgctcgc cttgcagcca gcgatttgtc ggcaatttca ctccccatgc gctcgacgat
    12661 atcggaggcc tgttcaaggt tgagcgacat ttcgatgatc tccgcccagc ggcgcgactc
    12721 ttcctccgcc agctcttctt ttggcatccg cgccagatac agcttaatgg cggtatagag
    12781 aacgttgata tcatctgcca gcttacgcag ctctttctcc tggcgtggct cgccgtgcat
    12841 cactttattc agcccttcca tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg
    12901 cgcggcgttc gccagagcaa gcgtgggtgt atccagcgcg ctgacatcca gatgtttagg
    12961 ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa accgtgccat
    13021 cggatcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga cgtggaaata
    13081 gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca gcaaatggac
    13141 aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca gcgcgacacg
    13201 gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt
    13261 agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg cggtcagagt
    13321 cgcagtcagc agtacagcag caaggctgga gtagctgata atggcgaaca tcgcgccaat
    13381 cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc
    13441 ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagtaaaa tcagcccaag
    13501 accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat
    13561 cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga gaatacgcgc
    13621 cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg cgagggctac
    13681 cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc tctgtaccag
    13741 tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag
    13801 gacagtacgc aaacgtgcgc cgaagacgcg cattacgccg gttcgaacaa tatgagtccc
    13861 ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga gttcaggttc
    13921 tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa acacgctgac
    13981 gggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt ccagcctgtt
    14041 tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg ccctgtttgt
    14101 tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat tcggtgccag
    14161 ccagcgctca acatcggaga cgctcatacc tttacggcgg gcatagtctt caacctgatc
    14221 gcgctgaatt tgcgccaccg cgtagtactt gctgtccggg tggctgaaat accaacccga
    14281 aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg tatgtttttc
    14341 cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg ccgggtagcc
    14401 cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc tgaggttctc
    14461 gttcggcgca tagccccagt agactttacg cacacgctca tggagatact ccgcaaaggc
    14521 ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat cgtgctgcgc
    14581 ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat
    14641 gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt agttagcgaa
    14701 gcccgttttt tcggtctgtt gtcgtagatg gtggctgacg ttgatcacat gggtacgcgt
    14761 ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca ggcccaccac
    14821 gccacgcgga ttcagcgttt tctcggcgct taatttatcc agcatgtcgt tagcgtcttt
    14881 aaacagccgc tgcgcctcaa cgcctaccac ttcatcttcc agaatgcgcg gatacttccc
    14941 ggccagcgac caggtcataa agaacggtgt ccagtcgatg taattacgca gcgtttcgat
    15001 gctggcttcg acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt aagcctgcca
    15061 gtcaaaagcg aagtcgttat cacgcgccgc ttccagcgtg accggtggtg tgcgcggttt
    15121 cttgcgcccg tgctgaatac gtacggtttc gtactccttg cgggtacgag caacaaaatc
    15181 atcgcgctga gtatcggaaa gcagcgccgc caccacacca acggtgcgcg aggcattctg
    15241 cacatacacc gtcgggccgc tgtagttctg ctcaattttc accgccgtgt gcgcttttga
    15301 ggtcgtcgcg ccaccgatca gtaacggaat ggtgaagccc tgacgctcca tctctttcgc
    15361 cacgttaacc atctcgtcca gcgacggcgt gataagcccc gaaaggccaa tcagatcagc
    15421 attcacttct ttagcggtac ggagaatttt ttccgcaggc accatcacgc cgagatcgac
    15481 aatttcgtag ttgttacatt gcagcaccac accaacgata tttttaccga tgtcgtggac
    15541 gtcgcccttc acggtggcga tcaccatctt gccgttggtt ttgccctgct ctttgctggc
    15601 ttcaataaac ggttcgaggt aggccaccgc ctgcttcatc acacgcgccg atttgaccac
    15661 ctgtggcagg aacattttcc cttcgccaaa cagatcgccg accacgttca tgccgtccat
    15721 caacgggcct tcaatcactt caatcgggcg cgtagcctgc tggcgggctt cttcggtatc
    15781 ctgctcgata aactcagtaa tgcctttgac cagcgagtat tccagacgtt tattcacttc
    15841 ccacgagcgc cactccgcct gctgggcgtt agcggtgtcg tcggttttgc tgccgcgata
    15901 tttctcggca agctccagta aacgctcagt gccatcatcg cgacgattaa gaatcacatc
    15961 ttccaccgcg tcgcgcagtt cagcgggcag gtcgtcgtaa atcgccagtt gcccggcgtt
    16021 gacgatcccc atatccatgc cattgcgaat agcgtagtag aggaacaccg cgtgaatggc
    16081 ttcgcgcacc ggatcgttgc cacggaatga gaaagaaacg ttagatacgc cgccggaaat
    16141 cagcgcgtgc ggcagttcgc gtttgatgtc ttcacacgcg ccgataaagt cctgcgcgta
    16201 gttgttgtgc tcttcaatgc cggtggcgac cgcgaagatg tttgggtcga agatgatatc
    16261 ttctggcggg aagccaacct cttcggtgag gattttgtac gcccgacggc aaatctcgat
    16321 tttccgtgcg cgggtatcgg cctgtccctg ttcgtcaaag gccattacca ccaccgctgc
    16381 accgtaacga cgcaacagtt tcgcatgatg gataaaggcg tcgacgccct ctttcattga
    16441 gatagagtta acaatgcctt tgccctgaat acacttcagc cccttttcga tgacctccca
    16501 ttttgaggag tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg caatcagatt
    16561 gagaaaacgc accatcgccg cttcggcgtc gagcatccct tcatccatat tgatatcgat
    16621 aatctgcgca ccgttttcca cctgctggcg ggccacatcc agcgcctcgc tgtatttctc
    16681 ttctttgatc aggcgcttga acttagcgga accggtgacg ttggtgcgtt cacccacgtt
    16741 cacaaacagg ctatcgtcgc caatgttcag cggctccagg ccggacaaac ggcaggctac
    16801 gggaatttca ggcagtttgc gcggcgctaa tccttctact gcacgactca tcgctgcaat
    16861 atgttgtggc gtggtgccac agcagccgcc gacgatattg agaaaacccg cttgcgccca
    16921 ttcacgtatc tgttttgcca tcgtgtcggc gtcaagatcg tactcaccaa aggcgttggg
    16981 tagcccggcg ttcgggtgcg ctgtgacgta gcattccgca atccgtgaca gctcctgcac
    17041 gtactggcgc agttcgtctg gccccagcgc acagttaagg ccgaaagtca gcgcttcggc
    17101 gtggcgcaga gagttataaa acgcttcggt ggtctgcccg gagagcgtgc gcccggaggc
    17161 gtcggtgatg gtgccggaga tcataatcgg cagctcaacg cccagcgctt caaactccgt
    17221 tttcaccgca aagaccgccg ctttagcgtt aagggtgtcg aaaacggttt caatcaggat
    17281 cagatccgcg ccaccttcca ccagcgcttt ggtggattcg cgataggccg ccaccagctg
    17341 gtcaaaagtg atgttgcgga atgccggatc gttgacgtcc ggagaaatag acgccgtgcg
    17401 gttggtcggg ccgagaacac cggcaacgta gcgcggtttc tctggcgtgc gcgcggtcca
    17461 ctcatcagca caagctcgcg ccagtttcgc cgccgcaaag ttgatttccg ccgacaggga
    17521 ttccatctgg taatccgcca tcgcaatggt cgtggagttg aaggtgttgg tttcgatgat
    17581 atccgcgccc gcttcaaagt aggcgttgtg gatagcggcg atcacttccg gtttgctgag
    17641 caccagcagg tcgttgttgc ctttgaggtc gcatggccag tcggcaaagc gttcaccacg
    17701 aaaatcggct tcgttcagtc gatagctctg gatcatggtg cccataccgc cgtccagcac
    17761 cagaatacgt tcatttaact gcgcacgcag ttgttccact ttgctgctca cacttgctcc
    17821 cgacacgctc aacccagatt taataaaaat tcaacaaacc atactggcat aaacgcatct
    17881 gtggtaaaag cgaccaccac gcaacatgag atttgttcaa cattaactca tcggatcagt
    17941 tcagcaacta ttgcattagc taacaataaa aatgaaaatg atttccacga tacagaaaaa
    18001 ggagactgtc atggtcgcac ccattcccgc gaaacgcggc agaaaacccg ccgctgccac
    18061 cgcccccgct accgggcaag tacagtcttt aacgcgtggc ctgaaattac tggaatggat
    18121 agccgaatcc aatggcagtg tggcactcac ggaactggca cagcaagccg ggttacccaa
    18181 ttccacgacc caccgcctgc taaccacgat gcaacagcag ggtttcgtgc gtcaggttgg
    18241 cgaactggga cactgggcaa tcggcgcaca tgcctttatg gtcggcagca gctttctcca
    18301 gagccgtaat ttgttagcaa ttgttcaccc tatcctgcgc aatctaatgg aagagtctgg
    18361 cgaaacggtc aatatggcgg tgcttgatca aagcgatcac gaagcgatta ttatcgacca
    18421 ggtacagtgt acccacctga tgcgaatgtc cgcgcctatc ggcggtaaat tgccgatgca
    18481 cgcttccggt gcgggtaaag cctttttagc ccaactgagc gaagaacagg tgacgaagct
    18541 gctgcaccgc aaagggttac atgcctatac ccacgcaacg ctggtgtcgc ctgtgcattt
    18601 aaaagaagat ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg aggaacatgc
    18661 gctggggcta cgttgccttg cagcgtgtat tttcgatgag caccgtgaac cgtttgccgc
    18721 aatttctatt tccggaccga tttcacgtat taccgatgac cgcgtgaccg agtttggcgc
    18781 gatggtgatt aaagcggcga aggaagtgac gctggcgtac ggtggaatgc gctgactttt
    18841 tctggcgggc agaggcaata ttctgcccgt catacctgag tggcaataga ataagggtgt
    18901 ctgttaatcg cattgacgcc aaaataactt attgtcatac acttcactca cttttattaa
    18961 ctcagcatta tttttaaaca tcaaaccact taattattaa caaaatcgca agatctcatt
    19021 ccaccgaaac ttttacggct gcattatgag attgagcgat ttatgatatt tctttacttg
    19081 ttatcatatt aaattttttc actgttctga tagttaaaat tcaagacatc aataaacaat
    19141 gagatattta aatgattact cgtattcctc gcagttcttt ctctgtaaat attaataata
    19201 cagcccagac aaatgaacac caaaacctga gtgaattgtt ttataaagaa ctcgaggata
    19261 aattttctgg caaggagctg gcgacgcctc tattaaaaag cttctcagag aactgtagac
    19321 aaaacggtcg tcatatcttt agcaacaagg attttgtcat taaa
//
LOCUS       DAZFFG010000088        19242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFFG010000088 DAZFFG010000000
VERSION     DAZFFG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.088"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..878)
                     /locus_tag="ACHJ7J_003506"
     CDS             complement(<1..878)
                     /locus_tag="ACHJ7J_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5001691.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKNT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDPEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGA"
     gene            complement(939..1511)
                     /locus_tag="ACHJ7J_003507"
     CDS             complement(939..1511)
                     /locus_tag="ACHJ7J_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5001692.1"
                     /translation="MKTGSEAIRALAMQIPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(1508..2251)
                     /locus_tag="ACHJ7J_003508"
     CDS             complement(1508..2251)
                     /locus_tag="ACHJ7J_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5001693.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            complement(2257..2955)
                     /locus_tag="ACHJ7J_003509"
     CDS             complement(2257..2955)
                     /locus_tag="ACHJ7J_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5001694.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGSFLSINKLSQ"
     gene            complement(2955..3284)
                     /locus_tag="ACHJ7J_003510"
     CDS             complement(2955..3284)
                     /locus_tag="ACHJ7J_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5001695.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGCSQRAPAGLNANLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(3281..5860)
                     /locus_tag="ACHJ7J_003511"
     CDS             complement(3281..5860)
                     /locus_tag="ACHJ7J_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5001696.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMTRVRRHFSGLETDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAISMPMVCVTSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVSEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADKTAQAFKSMWDAVLDIGRPDSSADMLAKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEKRENARLGLSAATLQADLEKASEMAAKD
                     RAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGERQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAENQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQ
                     QADKFAQQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSG
                     GGR"
     gene            complement(5841..6254)
                     /locus_tag="ACHJ7J_003512"
     CDS             complement(5841..6254)
                     /locus_tag="ACHJ7J_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5001697.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDQDMHPADFSLLATWREEEQTEMPDEEDMLMQKAAGLAGGVRFCGVGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            complement(6281..6712)
                     /locus_tag="ACHJ7J_003513"
     CDS             complement(6281..6712)
                     /locus_tag="ACHJ7J_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5001698.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAEE
                     VVLCLSGMIEAVRPDTDITEVAKNNVLTDDDFSAGKSSTAS"
     gene            complement(6728..7477)
                     /locus_tag="ACHJ7J_003514"
     CDS             complement(6728..7477)
                     /locus_tag="ACHJ7J_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5001699.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDQGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            complement(7485..7880)
                     /locus_tag="ACHJ7J_003515"
     CDS             complement(7485..7880)
                     /locus_tag="ACHJ7J_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5001700.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(7877..8452)
                     /locus_tag="ACHJ7J_003516"
     CDS             complement(7877..8452)
                     /locus_tag="ACHJ7J_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5001701.1"
                     /translation="MAIKGLEQAVENLSRISKTAVSGAAAMAINRVATTAINQSSSQV
                     ARETRVPRKLVKERARLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPESRYAKGNDDKGRKKRNRLPVQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELSYALKQQLSIAIKR"
     gene            complement(8464..8817)
                     /locus_tag="ACHJ7J_003517"
     CDS             complement(8464..8817)
                     /locus_tag="ACHJ7J_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5001702.1"
                     /translation="MRDFQNVFDAALAGVDSTIVEVMGLCALFTSGAQRGGEVQGVFD
                     DPESLGFASNGIRIEGSSPSLFVLTDTVRAVRRGDTLTINGEVFWVDRVSPDDGGSCY
                     LWLNRGQPPAASRRR"
     gene            complement(8810..9193)
                     /gene="gpFI"
                     /locus_tag="ACHJ7J_003518"
     CDS             complement(8810..9193)
                     /gene="gpFI"
                     /locus_tag="ACHJ7J_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5001703.1"
                     /translation="MATKEENLNRLHELAGLLGREADMSGSAADIALRVSEWEEELAA
                     SRERIMHADESVSEQNYTDDGRQLNNTDIPDDVKAVRVHKCLHVMGYCPETGRPVELT
                     FRGMRVLVPSTLATAMIQHGTAEYA"
     gene            complement(9245..10273)
                     /locus_tag="ACHJ7J_003519"
     CDS             complement(9245..10273)
                     /locus_tag="ACHJ7J_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5001704.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSHLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEQHYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(10331..10678)
                     /locus_tag="ACHJ7J_003520"
     CDS             complement(10331..10678)
                     /locus_tag="ACHJ7J_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5001705.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(10715..12220)
                     /locus_tag="ACHJ7J_003521"
     CDS             complement(10715..12220)
                     /locus_tag="ACHJ7J_003521"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5001706.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSRVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILVAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQVDAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(12210..13802)
                     /locus_tag="ACHJ7J_003522"
     CDS             complement(12210..13802)
                     /locus_tag="ACHJ7J_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5001707.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQVTQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSSRASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            complement(13799..14005)
                     /locus_tag="ACHJ7J_003523"
     CDS             complement(13799..14005)
                     /locus_tag="ACHJ7J_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5001708.1"
                     /translation="MVTVAELQALRQARLDLLTGKLVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            complement(13989..15917)
                     /locus_tag="ACHJ7J_003524"
     CDS             complement(13989..15917)
                     /locus_tag="ACHJ7J_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5001709.1"
                     /translation="MNTSEKQLNNFAVVCGVALRGLLRPLPVTTVEWADQNYYLPPES
                     SYLAGRWKTLPFQVAIMNCMGNDRIRTINLIKSARVGYTKMLMGVIGYFIEHKNRNSL
                     LFQPTDSAAEDFMKSHVESTLRDVPCLKILSPWLGRKHRDNTLTLKRFTSRVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSC"
     gene            complement(15889..16437)
                     /locus_tag="ACHJ7J_003525"
     CDS             complement(15889..16437)
                     /locus_tag="ACHJ7J_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5001710.1"
                     /translation="MNVNKKKLADIFGVDVRTITAWQSQGLPLVSGGGKGTESVFDTT
                     AAIQWYAQREADIENEKLRKEVEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     RDSGQVIDTGFCLFALGRLAQEISAILDSIPLSMQRQFSALTPAMLDFLKTDIAKAAN
                     RCASTAEKLPEMLDEYLREAAK"
     gene            16817..17056
                     /locus_tag="ACHJ7J_003526"
     CDS             16817..17056
                     /locus_tag="ACHJ7J_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5001711.1"
                     /translation="MSSKNKTSRTTTRNIRFPNRMIEQIHIALEEKGSGNFSAWVIEA
                     CRRRLSTEKRIKTAQRNTEELNTRSGYFYHRTNGQ"
     gene            complement(17268..17450)
                     /locus_tag="ACHJ7J_003527"
     CDS             complement(17268..17450)
                     /locus_tag="ACHJ7J_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5001712.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCASKQSVSQCVKPPPPPAWIMQPPP
                     DWQTSLNGIILPSENE"
     gene            complement(17667..18200)
                     /locus_tag="ACHJ7J_003528"
     CDS             complement(17667..18200)
                     /locus_tag="ACHJ7J_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5001713.1"
                     /translation="MNAKIRYGLSATVLVLIGAGASAPQILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNINVLLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            18329..18646
                     /locus_tag="ACHJ7J_003529"
     CDS             18329..18646
                     /locus_tag="ACHJ7J_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001714.1"
                     /translation="MNDKDNLHRVFMLVLNEKVKSEFLILLKDNYNVTKSIIAEPYGR
                     LSTETLDIIKTALTPLILQQLKHNINEWINEEFSSCNCLWDKSHACLQKKRLFNKLSL
                     KFR"
     gene            complement(18740..18955)
                     /locus_tag="ACHJ7J_003530"
     CDS             complement(18740..18955)
                     /locus_tag="ACHJ7J_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5001715.1"
                     /translation="MSPMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LALGFLTYLTNLYFKIKEDRRKAARRE"
BASE COUNT         4337 a         5882 c         4551 g         4472 t
ORIGIN      
        1 gcaccaagac gtttccccat gccgtagcgc ggatgggtca gcatatccca cagacaccag
       61 gccatgttgt tgctgtatgc cggtttaaac gttccgtccc agataccgct gtattgccgc
      121 gtctgcgggt tatagttcga cggcacctgc agaatgcgcc cgcgcagatg ataattacgg
      181 ctcacctgct ggctgccgaa ctgttccggg tccacctgca cgccgaccag tgccgtgttc
      241 gggtagcact gtttcacatc gatgatttcg gtgtatgacg accagagcgt tttgttctgc
      301 agctggtctg tggtgctgtc cggcgtcatc ctgcgcatcc ggatactgaa cgggcgcggc
      361 ggcaggttac ccaccaccac cgaggccaga tactgtgagg tgttttttcc cttaatggtg
      421 atgtcttttt ccgtcaccca gccaccgtta cgttgtatct gaaccagcag gcggacttcc
      481 gatggattcc tgtccccctt tgaggtggtt tccaccagtg cctgcacgcc gaaagtaaaa
      541 cgcagacggt caatgtttgc cgacgtgatg gtccgggtga tcggcgtgtc atatttcact
      601 tctgtaccga gcaccgtctc ggagccggag gattcaaatc cctccggcgg tgtctgctcc
      661 tgctcaccgg cccggaacac caccgtgaca ccggagatat tggtattccc ctcactgtcc
      721 agcaccggcg tactgttcag cagcacgctt tttaatccat ccaccggacc ttcaatcggc
      781 ccttcgctga tggcatcgat cacactcagt aactgcgtgg acttcaggtt gtccttcgct
      841 tcgcgcgggg tatgcccctt actgctgcct ttacccattc gtcacgctcc ataaacgaca
      901 aaaccgcccg caggcggttt cacataaaat gttttgcatc agcgaccaat caccacaacc
      961 tgaccaccat ccccttcgtc tgccgtactg atctcctgag aaaccacacg tgaccccacg
     1021 cgcatttcac cgtacagaac aggcagaaca ttgccctggg caaccatgtt atccagtgag
     1081 gagaaatagg tgttctgttt gccgttatcc gttgtctgtg tacggggagt tctggctttc
     1141 ggtgccagca tctgcgccac accaccgagc accatactgg caccgagaga aaacaggatg
     1201 ccggtcatac caccggcccc aatggctgcc ccccatgctg caagggtggc tccggcggta
     1261 aagaatgatc cggcaatggc ggcagccccc aggacaatct ggaatacgcc acctgacttg
     1321 gccccggcga ctctgggaac aatatgaatc acagcgccat caggcagagt ctcatgtaac
     1381 tgcgccgtta acccggacgt gctgacgtcc cgcccggcaa tccgtacctg ataccagccg
     1441 tcgctcagtt tctgacgaaa cgccgggatc tgcatggcca gcgcccggat ggcttcggac
     1501 cccgttttca ctcgaaggtc gatgcggcgg ccaaatcgtt gtaaatcccc gtaaaggcag
     1561 atgcgtgcca tgcccggtga cgccagaggg agtgtgtgcg tcgctgccat ttgtcggtat
     1621 acctctctcg tttgctcagt tgttcaggaa tatggtgcag cagctcgccg tcaccacagt
     1681 aaatggcggc atgattcggc accgatgaac caaaacagca cagcagcaca tcgcccggct
     1741 gcgccgatga caacggcacc tgatacagcc ccgttgcttc cagattatcc agatagagat
     1801 tctgaccgtt acgccaccag tcatccccgc gatgaaaatc cggcatctca atccccgcca
     1861 gatgataagc atcccggaac agcgtgtaac agtccgtcac cccgtgctca aagcgccgcc
     1921 cggtaagatg cggcacacag cggaacttgt gaatctcacc ccggcagacc agccaccaag
     1981 gcaaatcgct ctgcacctgc agccgcctgt cagcctcact cagccagggc agaccaccgg
     2041 ggtggctgtg gaccagcgca acaatctcac cctgcatctc tgcccgcagc cagtcctccg
     2101 gcgacatccg gaaatacgcc tccggctcac cggagatatt cacgcaggga aaatatcttt
     2161 ccccttccgg cgttctcacc acgaagccgc acgactccgc tggcgcacat cgccgggcgt
     2221 gcgccagaat cgctgattct gtctctgtca tgggatttac tgcgaaagtt tgttaatgga
     2281 aaggaagctg ccaaagttgc cgacattatt gcgaaactta cagccactca ggcatttgct
     2341 gcatttatcc ttcgtgatat cggacgtcgg ctggtcatat tcatccgcga ccgccggacc
     2401 gtgataaccg cactcatcac cgcgataggt ccaggtgcag gtgttggcca gcatgatgcg
     2461 ccccggaaaa acagcaccat ccgtttccgt cggtgtggag agcacaaagg aggcactaac
     2521 cgcactcagt tcgctgcact gctcgatgcg ccagcggctg atcacctcct gctccggatc
     2581 ggcgtcgctg tttccgttga cgaagttcac cgcatccaga aaacgggcgt aaaccttacg
     2641 ccggaccacc gttccgccga ccagactctg caggtcttcc gccatcccgg tgaccatgcc
     2701 gtgcaggtta gagaccgtca gtgtcggacg ggcagcactg cccttaccgt tcagttcaaa
     2761 tcccgtcccc tgaatggggt atgcctgata ctgccgcccc tgccaggtaa ccggctcacc
     2821 tttttcgttc tgctcattac agaaaaaata acgttcacca ccgacctctg tcagatcgat
     2881 ttcccagagc accacgctgg ccgactgctc cgcacgggtg cattcattca gtgtttcctg
     2941 ccggatatcc tgcatcagtt caccacctgt tcaaactctg cgctgaactc aacacgcagc
     3001 atactgaccc gcgacgacca ttttgcgcag gtcaccttta tctgccgcca ctcataaggc
     3061 ggcgtccaca gaaaggcctt ccagcccccg tgctcagcca gaaacgactc aagcgccgtg
     3121 gcctcccaac ggggaacaga aagcgtcacg ctgtacgttt tcaggttggc attcagcccg
     3181 gcaggcgctc gctgggaaca gccatcacca aagcgcacct ttcttacaga aggggccgaa
     3241 gccacatcca taccgggttt cactttccag cggaaggttt tcatcgtcca cctccggaga
     3301 acagaccacc atcacgcatc tgcccggtca caacatccat tgccgcctta cgggctacgt
     3361 cataaaccgc cttcagtgcc tgtggcccta tctgaccgtt cgtgccgtcg ttgttaatca
     3421 ccacatggtt attctgctca aacgtcccgg acgcctgcga ccggctgtcc gccatgctgc
     3481 ccggtgtacc gacataaccg ccggtggcat agccgcgcat cagccggtaa agatttccta
     3541 cgccaatccg gctggttgcc tccttcgtga agacaaattc accacggtga acaatccccg
     3601 ctggctcata tttgccgccg gttcccgtaa atcccccggt cgcaaaatgg aatttcgccg
     3661 cagctgcctg aatggctgta ccgcctgacg cggatgcgcc gcccccaaca gccccgccaa
     3721 tggcgctgcc gatactcccg acaatcccca ccattgcctg cttaagcaga atttctgtca
     3781 tcatggacag cacggaacgt gtgaaactgc gccagttctg ttcactgccg gtcagcatcg
     3841 ccgccatatt ctgcgcaata ccatcaaagg tctgcgtggc agcactttta acctgcgaca
     3901 tactgtccgt ggcgctctct tcccactcac tccagcctga tctgaggcct gccatccagc
     3961 tcccgcgaag ctggtcttca gccgcccagg tctttttctg ctctgacatg acgttattta
     4021 gcgccagcgg attatcgcca tactgttcct tcaggcgctg ttccgtggct tcccgcgctg
     4081 cctgccggtc agtcagcccc cggttttttg catcaatggc ggcccgtttt gcccgttgtt
     4141 gctgtgcgaa tttatccgcc tgctgcgcca gcgcgttcag gtgctcctga tacgtgacct
     4201 tatcgccaag tacagccagc tggcgtttgt actccagcgt ctcatcttta tgcgccagca
     4261 gggatttctc ctgtgcggat agctggcgac gttgtgccgc ctcctccagt accgcgaact
     4321 gattttccgc cttccacaaa tcccggcgct gctggctgat tttctcattc gctccggcat
     4381 gcttctccag tgtccggagt tcagcctgaa gcgtcagcag ggcagcatga gcactgtctt
     4441 cctgacgctc tcccgcagac accttcacgc cggactgctt cggctttttc agtgtcgctt
     4501 catagtcctt tttcgccgcc gccatcagcg tgttgtaatc tgcctgcagg atttttccgt
     4561 ctttcagtgc cttgttcagt tcctcctggc gggcggtata tttctccagc ggtgtctgca
     4621 ggcgttcgta agccttctgc gcctcttcgg tatatttcag ccgtgatgcc tcagactcgg
     4681 cccgatcttt tgctgccatc tcactggcct tttcaagatc tgcctgcaac gtggcggctg
     4741 aaagcccaag tcgcgcattc tctctcttct cccatgcacc ccggagattg gcaagaaatg
     4801 ctgacgtttt accgcgacgg tggcttcggc tctgatacca ctgccatttt ttatccgctt
     4861 catcaaaagc cttttcagct ttggcgagca tatctgctga ggagtccggg cggccaatat
     4921 ccagcaccgc atcccacatg gatttgaatg cctgtgcagt cttgtctgcc caggtctcca
     4981 gcgtgcccat gttctctttc aggcggcggg tctggtcatc aaaccctttc gttgcggcct
     5041 cgttcgccgc ctgtaatgcc ccggcttcat ctccggaacg ctgcaactga gcaacatacg
     5101 caatctgctc cgccgtcacg ttatggaact gacgtgccat cgctgtcagc cctgacgtcg
     5161 ggtctgtagt cagcttcccg aaggcttcag agaccttgtc cacctccacg ccggatgcag
     5221 aggagaaacg cgccacactc tggctgatgg acgcaatctg agcctcaccg cttacccccg
     5281 ccttaaccag tgcgctgagt gactcgctgg tctggttaaa cgtcagccct gccgcctgcc
     5341 cggctctgga caggaccagc atacgatctg ccgtcagacc cgactgattg ccggaaagga
     5401 ccagcgtttt gttgaaatcg gacagggttg agttgccctg ataccaggca tacgccagcg
     5461 caccggtcgc caccgccagc gaggtgacac agaccatcgg catgctgatc gcaccggcaa
     5521 gcccccggaa catggggatc atcccgccga aggagtcctt aacctgcccc ccctgttgca
     5581 gcaggatgag ccagggattc tgaccaccgg caagctgggt agcgatatcc gtaaactgtg
     5641 caggaagggt acgcatggcg gctttatact gcccgacgga aatcccggct ttttgtgcag
     5701 ccagcgcctg gcggctcagc ccctgctcaa cagcactggc ggtttttctg gcgtcagtct
     5761 ccagtcctga aaaatgacgc cttacgcggg tcatctgctc atcgaagcgg accgcatcca
     5821 gactcaggtc aataacaaga tcaccaaccg gctgggacat atctcacacc tccggaaatc
     5881 cccgctgaag ccatcattaa tgcaacatca tcttcgctga catccaccac atccgcagaa
     5941 ggtgaaatat cgccccctcc caccccacag aaccggacgc ctccggcaag tcctgccgct
     6001 ttctgcatca gcatgtcttc ctcatcaggc atctccgtct gctcttcctc tcgccaggta
     6061 gccagcagac tgaaatccgc cggatgcata tcctgatcgc aaaaaaacag gctgagtacg
     6121 gcgtacgtca gcccggaaaa atgcatatcc agttgggtat cctgaaaata atgcgtgcgg
     6181 taaaaacggt gccagtcggc atattcggtg gatgtcatcc cggcaagcat ggcgcgccag
     6241 tcgggtctcc ccatctcacg cgccagtcta agggcaaaat tcagctcgcc gtcgaagact
     6301 ttcccgcaga aaaatcatca tcagtcagca cgttattttt cgccacttcg gtgatatcag
     6361 tatccggacg aacagcttcg atcatcccgg acaggcacaa caccacttct tccgcccggg
     6421 caatggcatc tgcaggccag gtagtgagca cttcctgctc tatcttcatc acggcctcat
     6481 tcattgacgg tgactgcgtt ttctgtggat ggttatgcca cagggacatc gccaccagaa
     6541 acgccccggt tctgacaagg tcttccacgc tcacctgcag gttgccgctg gcttcagcct
     6601 cttctgcccg ccgtttcagg agggcaagat gctcaatacg ctgcagcgca gacagttcgg
     6661 aaagggtgac ggacacgccg ttatattcaa attgttctgt tttcagaaac atgctttatc
     6721 tcccccctca gcctgcagcg ccatccgtga cggtgatctc cgccaccgcc gcaaactcac
     6781 cattgccggt gacaacaggg atctgcgctt tacctgccgc aacacctttc acggtgatcg
     6841 tgttcccttt cacggtaatg gtcgcaaaat tctgattcgc cgacgtggcg cggaaggttt
     6901 tatccgtcgc cccttccggc tgaacagcca cggtcagggt gatattctga ccttttgcca
     6961 cattgcccgt tggtggcgtc acggtaatac cggtgaccgg tgtgatgtcc ccctgatctt
     7021 ccgccagcga cggacgaccg atattggtga tcttcacggt acgggtgatc acctctttgg
     7081 cggtcaccgc tttaccaatg gcgctcaccc agccacggaa cacatccacc gtgccattcg
     7141 ggaaacggat tttgtaggcc cgggtctcac tgctgtcaaa ccaggcgatc aaatcgcgct
     7201 gccctttctc gccgggcttc caggccagag taaaactggt atcaccggcg gatttctgcc
     7261 cctgcccggt ggatacccag tcagcatcct catcatccag atagttatcg tcgtaggatt
     7321 ccgccgtcat ctcgccgggg gtcagatcct ttatttttgc cagacgcgtc cactcatcat
     7381 ctgacagcgg gtttgcataa gcatcaccct taccggtgta aacccagagc gtggtgccgg
     7441 aaccttttac cggcgccagg ggatttggtg ttgccatatc atcctcacat ctcgtatgta
     7501 atggaataag tcagatctgc agagctccac aatgccatat cgtcatcacg acgatactca
     7561 tagccctgcg taaccatcgt ggtaatcatg cctgccagtg cagggatcgc cgccatcgcc
     7621 gggtaaatcc ggctttccat ccactgatca agctctgaat ccggtacctg tgccggtaaa
     7681 aacacctcaa tatgcagtgt ggcccgccag gtatcagcat ccagctcttc accggtatac
     7741 tctgcatccg tcagataaac cgcgaccgcc ggaaaatccg cctcctcaaa aacagcgggg
     7801 cgaccatcaa acagcgtcgc cccggtgtca tgcttctcca gtgcatccag tacggctgca
     7861 cggatatcag tatgtttcat cgttttatcg caatactcag ttgttgtttc agcgcatagc
     7921 tcagttcttt tggcagacgt tcgcgtcgga tacggttaac gttctcatca aaagcctgtt
     7981 tcagtggggc cgccatcgga attttcacca cctgaacggg aagacgatta cgctttttcc
     8041 ttcccttatc gtcattgccc ttcgcatacc tggattctgg cagacgttgc ataacatgcc
     8101 agcgcccatt attcagtcgc tggatgaatg cccgctgata acgatgctga ccggctttga
     8161 gtatgctgtt cgggcgatga ccaagcatcc tgatccccag cttaataact gggagatcac
     8221 cgcggttaac gataattttt gcattcggat ttctgaccgt cgcccgtttc agtctggctc
     8281 tctctttaac cagttttctc ggcaccctgg tttcacgggc gacctgagac gaagactgat
     8341 taatcgccgt tgtggccacg cggttaatgg ccattgcggc agcaccggat accgccgttt
     8401 tgctgatacg gctgaggttt tcaacggcct gctcaagacc ttttatggcc atacatcccc
     8461 ctttcagcga cgacggctgg cagcaggtgg ttgcccccgg ttgagccaga gataacaact
     8521 acccccgtca tccggagaaa cacgatccac ccagaacacc tcaccgttaa tggtcagcgt
     8581 gtcaccacgc cgcacagcac gaaccgtatc cgtcagcaca aataatgacg ggctgcttcc
     8641 ctcaatacgg atcccattac tggcaaatcc cagcgactcc ggatcgtcaa aaaccccctg
     8701 aacttctccg ccacgctgtg cccccgaggt gaacagtgca cagagcccca tcacttcaac
     8761 aatcgtactg tccaccccgg caagggcagc atcaaagaca ttctgaaaat cacgcatact
     8821 cagccgttcc gtgctgtatc atggctgtcg ccagtgttga tggcaccaga acacgcatcc
     8881 ccctgaacgt cagctcaacg ggacggcctg tctccgggca atatcccatc acatgcaggc
     8941 acttgtgcac ccggacagct ttaacatcat ccggaatatc agtgttgttc aactgcctgc
     9001 cgtcgtcagt gtaattttgc tcagatacgc tctcatcagc gtgcataatg cgctcccggg
     9061 aagcagcaag ttcctcttcc cactcagaca cacgtagagc aatatccgca gcactccccg
     9121 acatatccgc ctcgcgcccc agcaggccag ccagttcatg aagacgattc agattttctt
     9181 cttttgttgc catctcagcc tcctgtgaaa aaagacacgg ggacatttcg cccccgctca
     9241 cggattattt cacctgcacc accacaaact catccgggtc cggcagcacc atcaacggcg
     9301 cggactgcgt catggtgaat tcacgggccg ggtcacccac cgtcagccag tgtttcgggt
     9361 aacgggaaga ggccaccaca ccttcagaca atgcctgcgc atcctgaatg gcaccgtagc
     9421 aacgaatacc ttctgccgcc gtatttccca gtaccagtgt cccatccggc agatagtgtt
     9481 gttcggtacc gtcctctgcc acataagacg ttttcgccac cacaatggcc agatcgccgt
     9541 aatacccttt aaaggacact accgcaccca gatctttcac tgccgtttcg aggtgtgaat
     9601 ttgagccgcg acgggtatcc agtttttcgc ggaacagctt aaaaccattc agcagacgcc
     9661 agacggtacc gtccatgatg gcaatattca caagaccgct ggcctgatcg cagtagaggt
     9721 caatatcatg cgtcggatca aacgtatcac ggtcctgctc agaccatttt ttaccgtcgg
     9781 cctgctcaat gttatttcct tcagagcgtc cgaaatcaac ctcgacagta tcaaactgct
     9841 ccccttccat ggtgtattta ccatacagca cagcatttac cgcctgcatt tcttccacct
     9901 ggacaatcgc gtgctcttcc tgtttgaggt tatcggtaat gatacgcaga cggcggtaag
     9961 ccggatcgtt cagttgagcc ggatcttcac caggaaggcg ctcaaccgcc tgctggtaat
    10021 taaattcgtg tttcggcttg acgtagcccg gacgcaacac gcgggtttca ccaccacgat
    10081 gacgcagcac ttttccttca acaaccgggg agacataggc cgccaccggc gtttttccgg
    10141 taattttgtc cagcatcacc tcttcggtat ggaaattcac cgtacggcgg aaaaacagct
    10201 ccagaaacag cgcacgaaat ttaacttttt gttcggtata accgagtaac tggcgggtcg
    10261 taaacaatcc cataaatcag ttcctttcat tcagaaatca gtcaggccac cgcggtggcc
    10321 tgataacgtg ttacggcagc gccgcgtgac tcagtgcact gccggcaaag gcgttggcct
    10381 ttttgtgttc atccacactt tcaggccagc ggattgcctc cgtcgcaaag gtccccgact
    10441 tgtaatacgt cagcgccgtc tctgtgcctt caagcggcag taccagtatg ccaaccgcac
    10501 taccggcttt ctgtccgtcc cagaccacca gtttcccggt ggcctcatcc agcatcaggg
    10561 gtgtcagtgc cggtgttgcc gaggaaatcc cgctgctgcc tgtggcggta tgagccggat
    10621 cattaccggc aaaaatacgt acttccgcac gctgttcagt gatggttttc gttaccatat
    10681 tgtaaaaacc tcctgttgat ggtcagcact ggcttcatgg catggccatg agcattttca
    10741 cgtccgcatc accatctgct gacgtctgtg gcacgccacc ctgtaccgct gccggtgaat
    10801 ggttcgccat gaaatgttca aacatggcgg ttgtggatgc agagaccggt tctgccttac
    10861 ctgatcccgc cgccagcaca gcccgggcgc tctccacagt cattcccggg caggcagcca
    10921 gctgttcagc ctgcgcctca gccccttttg cctcatccag tgccatgatc tgatcacgga
    10981 gtgatggtcc ggcatccacc tgcggtgaag cagccaggat cgggcgggct ttttccaccg
    11041 tcatctccgg catcgccgcc agcgttgccg ccagttgttc acgaccgttc gcttcttcac
    11101 acgccataat gcgatcggct tcactctgcg tggatgccac cggctgctgc ggtgccgccg
    11161 cgaccagaat cgcccgggcc tgttcaacgc tcataccctg ttgtcctgcc agcatcgtgg
    11221 caagctgttc acgtcctttc gcttcctggc atgtcaggat ccccatcact cgctggttct
    11281 cctgcgcggc ggcttccgtt gcagttaatt gcggcatagt gcctcctctg acattactgt
    11341 tcagcgccgt ggccatcaca ctgatggcat ccgacgcatt gattaattca tccgccagcc
    11401 cggcctcaat gccggactga ccttcaaaaa cggcagcctc tgttcccgtg acggcatcaa
    11461 cagacagacc ggtatacatc gccacttttt cggcaaacat ccggcgcgcc gcatcaatcc
    11521 gctgctgcat gtcctggcga acctctgccg gcaacgcttc aaattgattg ccatccacct
    11581 tgtgcgcccc tgagtaaatc agcgtgatat ccacaccggc ctgcgccaga tgaccggcat
    11641 agctgacatg gctcatcatc acgccaatgg agccgatacg ggatgtctgg gtaaccagcc
    11701 gtcgggagca ggccgacgcc agcagcatgg ctgcagaaca ggccgtgtca ttgcacagtg
    11761 cccagaccgg cttctgctga cggaggcggt aaatcatgtc agcgcagtca aacgcgccgg
    11821 cggcctgccc gcccggactg tcaatgtcca gcagtacgcc ccgcacccgg ctatccgcca
    11881 ttgcctgctg aagacaggcg acaatgccgt catagcctgt cattccggaa aatggccgca
    11941 taccacccag acggtgcacc agcgtgccgg tcaccggcag tacagcaata ccgttcacca
    12001 cccggtaaac acgggccggt cgtttacctc cggccatata ctcgtccgtt tcagccagca
    12061 ttccgggagc atcaagctgt acctgttgtt gtggtaccga aagacttgct gcccccatct
    12121 cgcgcccgag cgcgcaaaag aaaacccgcg cataggcagg ctccagaagc agcggttcat
    12181 tgaatgctgc ggcaataatg tgtgaaagat tacgtctcac gtggtgttgt ctcctcttcc
    12241 ggcctgcgac tctccgctat ctgctgctga tacgcctgcg ctatccacac cggacgtgag
    12301 agtccggctt tttcccgctc tgcagattcc ctgacctgct ggcggaaaat gtcctgataa
    12361 tcctcgccca tcagcgccag ctctttctca tacgtgctca gtccggcctc aatgcgcatc
    12421 actgattcct ggacttcctt gagcccgtca atggccattc ttccggcccc aatccactct
    12481 gcccgtgacc aggctgatcg cgcctgataa aaatcaaaac gtgcccgtgg cggacgaata
    12541 atcccccgaa gaagtgcctc ttccagccag caggaaaaca tctgcgtggc cagccgggcc
    12601 gcaataaatt ttcgccgccc cataaaatag cgccacgact cattggcgga ggcgcgggaa
    12661 cttgagtaac tgaccttcga gtagtcacgg gacaactgtt cgtaggaaac gccaagaccg
    12721 gcggcgatat accgcagcag cgcctgttca agcgcagaaa atccattgtc tgaatcctgc
    12781 gcggtctgca gtttcagatc atcccctggg aaaaggtgcg gaattttgac accgcccagc
    12841 gtcacgtgat tcgtgtcata ccagctggag aacttctcca gaatattaat aagcggatta
    12901 tccttctgcc cctgcggcgc accggcgata tattcaaagg ccttttcggt atcaagttca
    12961 ctttcgattg tggctgcata catggccttc acaatggccg actgaagctg tgttacctgc
    13021 agggaatcga gcatcttcag ccgctccatg acgctgtaaa actgattagc accacgggtc
    13081 tgcccgtcct ccaccggctc gaaaatatgc agcatggccg gacgcccggt gggaagttca
    13141 cgcgggatcc gttcccatcg tccactacca gagaacggaa aatcatcctc acagatatgg
    13201 tacgcgacgg cacggccata tcgatcgacc tccacaccgg cccgcagaaa acggttcccc
    13261 ataccgtgtc caggcgtgtc cacccgtttc ggactcacgg ctttaaaacg cgtacggaat
    13321 aactgcgtgg tttccgtatc ccagaccggc tgcacaaaga tttcgccgtt aaacgcatga
    13381 acgcccacac cttcacggat aaattccgtg aacgtgcgtt ttccttccac gtcgatctcg
    13441 ccaaacatcc cttcggcgta ttccgaccag gccgcctcca cctcatcgac aaagcttttt
    13501 gctgcggtct cccgcatccc cagccagcgc cagttcggac ggtagctgat cagaaacata
    13561 tgcccgacaa tgtgatcctt atgcagagcc accgcattag ccgctattcc gttattgcgc
    13621 accagatcat ctgcccgggc attccccaga cgcaacgcgg gcagcagggc cgcatcggca
    13681 ctctgcgccg gtggcaacca ctcagccatt tgcccgccaa atcctgcacc gcccccgttg
    13741 tagctgagac tctcacgaag cggaacgccg ttcacatcaa tcaggacagg cgttcgtttc
    13801 ataacctcac tcccagcgga cgacggcgac gtcgggttgt ccccagtacc gactccgcat
    13861 cattgatcgc ccggttaagc tcatccagag aagccgccgt atattcaatt ctgcgaccat
    13921 ctttctggac agacaccacc agtttaccgg ttaataaatc aaggcgcgcc tgacgcagcg
    13981 cctgcagttc agcaactgta accattcact cctccggaca gcttcgctgc cagttcttta
    14041 agggttggcc gggtcgtctc ttcttcccgg gattttgcca gtacagccag atcaagctgc
    14101 cagcgttgca cggacacacg taatgccgcg taggcataca ccaggcagtc cagcgcttcg
    14161 ttacgccgct ttttgttatc ccacagcaga cgcatctttc ctttttccca cttctccacc
    14221 agctcttccg ccaccagttg ctgcgcctct gtctgcgaaa aaatctccgg atcatcagga
    14281 aaacggatgg catacgacgt ggcttcatcc gcaggcgtgg gatcggcttt catacgggca
    14341 tagagaattt cttttgcggt gtcagtcccc acttcacaca gatacacgcc ccgctgattg
    14401 cgggtttttg gcatggtgat caccggcttg ccatagacag atgcgccttt taccggcagc
    14461 acccggaaaa caccgtgttt ttttgatctc tgataaacaa tttcaccatc gatccccccg
    14521 gtgtcccagc agacacggga aatggtcatt tcggtgccat ccgcatggcg gtattttttg
    14581 ttgatcgctg catccacacg taacagcgtc tcttcctcat cgggacgccc cataatgatg
    14641 attttatcca ccagaaaggc ttcctctccc ggagcccatc cccagacata catctcaaaa
    14701 cggtttcgct gcgagtcaat gcccgccgtc agataaacca cccgggcagg caccgccgcc
    14761 gtgtaacgca caaccttatc catcagtacc tggtgatcga gtttttcgcc cacggcctct
    14821 tcccaggtct cgcccagcgt ggtgttcaca aaggttttca ggccgttggg atctttcagt
    14881 gcatccagcc agtcatagac aatctgtacc caggtggtga acggactgta cgccgtccag
    14941 atatggaacg tgatggagcg cggcggcgga atttcattac ccgcagcgct gaaaaacgtc
    15001 agaccgtcac gggtccacat gcccgtgttt tcacagatcc accgcccgtt gctctggtcc
    15061 agttcagact gatggatcac acagccatga tgttcacaga ggtagaaaac actttcggga
    15121 ctgtccttct cccatttaag cccaaaaggc gtggactcat cgccaaattt cagatactgc
    15181 tcctccccac agtgcgggca gggcacataa aaacgcatga aatgcgccga ctcgttggcc
    15241 gctttttcga tctggcagga gccttttatt ttaggcgtcg agccgcgaat ggattttggc
    15301 catacagagc cctcaatacg cttatccccc agcagggttg gcgagccctc tttttcaaca
    15361 tccggctcga acgaggaaag ttcgtcatag cagaccacat ccacggattt ttcacggtag
    15421 tttttggcgg cagcaccacc caggcaccag aaacccacac gggaagtgaa tctttttaat
    15481 gtcagcgtgt tatcccggtg cttacgtccc agccaggggg aaagaatttt cagacaggga
    15541 acatcccgta atgttgactc cacatgagac ttcataaaat cttcagcggc agaatccgta
    15601 ggctgaaaca gaagactgtt acggttttta tgctcaataa agtaaccaat aacgcccatc
    15661 agcattttgg tgtaaccgac tcgagctgat tttataagat taatcgtgcg gatccggtca
    15721 ttacccatgc agttcattat tgccacctga aatggcaacg tcttccaccg cccagcaaga
    15781 taagaggact ccgggggcaa ataataattt tgatcggccc attcaacggt agttacaggc
    15841 agtgggcgta aaagcccacg aagtgccact ccacaaacaa cagcaaagtt atttagctgc
    15901 ttctctgagg tattcatcca gcatctccgg taacttctct gccgttgatg cgcatcggtt
    15961 tgccgccttt gctatatcgg ttttcaggaa atcaagcata gcgggcgtca gtgccgaaaa
    16021 ctgtctctgc atggacaatg gaatggagtc aagaatggca gagatctcct gtgccagtct
    16081 tcccagagca aaaaggcaaa accctgtatc aatgacctgc ccactgtcgc gggcattttt
    16141 cagttcctgt gcatctgcct gtgccttcgt cagtcggtaa cgttcgtaat caatggtgcc
    16201 gggctgaagg tctgattcgc tggcagccct caaatcctcg acctctttac ggagtttttc
    16261 gttttcaata tcagcttcgc gctgcgcata ccactgaatg gcagcagttg tatcaaaaac
    16321 tgattcagtc cctttccctc caccagaaac tagtggtaac ccctgactct gccaggcggt
    16381 gatggtcctg acatcaacgc caaaaatatc ggccagtttt ttcttattga cgttcataca
    16441 ctcatccgga aaccagaaag gatctgaaaa tggcgttttc ggacaaaaac agcctttgtc
    16501 agatcctttt atatttttaa tattctactg ataatcaata agttaaaaag aagaagaacg
    16561 gatctgcttt tcccgaaaaa ttttcataaa tagtgaaaac gcgcgaggtc gccgccccgt
    16621 agcctgtcgg atcgccggaa aggacccgca aaatgataat aattatcatc tacatgtcac
    16681 aacgtgcatc tacgccatca aaccacgtca aataatcaat tttggcgcag gtgtcgcatt
    16741 aattgatccg catcaactta acgtaaaaac aacttcagac aatacaaatc agcaacactg
    16801 aacacgggac aacatcatgt catcaaaaaa taaaaccagc agaacaacta cccgtaacat
    16861 ccgctttcca aaccgaatga tcgagcagat acatattgct cttgaagaaa aaggatcagg
    16921 aaatttttct gcatgggtca ttgaagcctg tcgccgaaga ctgtcaacag agaagagaat
    16981 aaagacagca caacggaata ctgaagaact aaatacccgg tcaggttact tttaccatcg
    17041 tactaatgga caatgacggg aaataagtcc tcacaccttg tacactgtaa aatccgatgt
    17101 gcatcgtttc gattatttcc gtaccaacga acatgaagtc aactacatca gtcaactatt
    17161 tctgttaata cggaaacaca gcgtcgatac agcgtatccg ggaacttatt tcagacattg
    17221 agtacggata tattcctgcg ctccttccag ttgtttctgc atcaccatca ctcgttctct
    17281 gagggtaaaa taatcccgtt cagcgacgtc tgccagtctg ggggaggctg cattatccac
    17341 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgc gcaaccgacg
    17401 acgaccagcg gcaacatcat cacgcagagc atcattttca gctttcgcat cggctaactc
    17461 cttcgtgtat tttgcatcga gcgcagcaac atcactctgg cgctgctgca tgtcagtgat
    17521 ggtcgcgttc gcctgactta gttttgtctt cgcatcatcg cgttgtacct tgtaggtgat
    17581 ggcgttatca cggtaatgat tgaccgccca cgacaggcag acgatgatgc agataaccag
    17641 agcataaata atcgcggcga ctctgctcac tgatctatcc cccaacaggc taatgcgctt
    17701 tcctggtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg gcaatcgcgc
    17761 ccaccatctt ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc accggcattc
    17821 agccgcttat agaacgttga cggaaaacat ttaccggggc caatgttata gggacagaat
    17881 gacgcaatac ccgctttttg tggttcggtc agtaatacgt taatattgcg ttccacccat
    17941 gccagtgcct tatcacgttc aatggcgtta acctggtcgc atttttcctt cgacaatttc
    18001 ataccgggaa aaacgggttt tccatccacc atcgtggcac cccgacagat ggtccagatg
    18061 ccggaaccat cacggtatgc cgttgtgtgg ttaccttctt tttcgtccag aaactggtca
    18121 agtatttgag gagcagacgc gcctgcacca atcagcacta gtacagtagc cgacaggccg
    18181 tatctgattt ttgcgttcat gtatatttat caggatttat cgtcttctga atcccggcta
    18241 tgttagatct gtcatcagag gctcataaca catctgatgc acatacccca gggctacacg
    18301 ccggatgacc aaagatgaag ctttgaaaat gaatgataaa gacaacttac acagagtatt
    18361 tatgcttgta ctgaacgaaa aagttaagtc agaatttctg atactcctca aggacaatta
    18421 taatgtaacg aaatccatta tcgctgaacc atatggccgt ctctcgacag aaacgctgga
    18481 tattataaaa actgcattga caccattaat acttcaacaa ctgaaacata atataaacga
    18541 atggattaat gaagaattca gttcctgtaa ttgcttatgg gacaaaagtc atgcctgcct
    18601 ccaaaaaaaa agactcttca ataaattatc actcaagttc agataaagtt aaaaatttgt
    18661 ttctgtacat taaagaagag catctccgat aaaaccagat caactggtaa ttttctgaac
    18721 aagagaatag ttcgctgagt cattccctcc gtgcagcctt acgcctgtct tctttaatct
    18781 tgaaatacag attcgtcaga taagtcagaa agcccagtgc cagacttccc agtacaccaa
    18841 ttgccgccca ctgtgacgga ctgacctgat caagccactg taaaaaccag tagccggcac
    18901 tgcctgcaga agtaccatag gcaatacccg ttgaaatttt gtccattgga ctcacgacct
    18961 cacctccgaa tcatccggat ggcgttatat gcaggaaaga gagttttatg acgaaaaaaa
    19021 agctgtcaga cgatgaagcc atctgacagc gatagcaggt aatccagaaa acatggacag
    19081 gaaagcaaaa caaacgagtt aagaagacac ggcgtcggat ttttaccgat gagccttggc
    19141 gtggtcaacc gtgctcgctc ccttgacttt tgatgctcat taaaatgatg aaaatcctta
    19201 ttacatcata ccacatcagc cctgctgcat tacaggatgc gc
//
LOCUS       DAZFFG010000089        19197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFFG010000089 DAZFFG010000000
VERSION     DAZFFG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.089"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            424..1458
                     /gene="tqsA"
                     /locus_tag="ACHJ7J_003531"
     CDS             424..1458
                     /gene="tqsA"
                     /locus_tag="ACHJ7J_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO5001716.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(1483..2871)
                     /gene="pntB"
                     /locus_tag="ACHJ7J_003532"
     CDS             complement(1483..2871)
                     /gene="pntB"
                     /locus_tag="ACHJ7J_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO5001717.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(2882..4414)
                     /gene="pntA"
                     /locus_tag="ACHJ7J_003533"
     CDS             complement(2882..4414)
                     /gene="pntA"
                     /locus_tag="ACHJ7J_003533"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO5001718.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            4938..5882
                     /gene="ydgH"
                     /locus_tag="ACHJ7J_003534"
     CDS             4938..5882
                     /gene="ydgH"
                     /locus_tag="ACHJ7J_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO5001719.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            6068..7450
                     /locus_tag="ACHJ7J_003535"
     CDS             6068..7450
                     /locus_tag="ACHJ7J_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5001720.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            7487..8209
                     /gene="folM"
                     /locus_tag="ACHJ7J_003536"
     CDS             7487..8209
                     /gene="folM"
                     /locus_tag="ACHJ7J_003536"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO5001721.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(8206..8541)
                     /locus_tag="ACHJ7J_003537"
     CDS             complement(8206..8541)
                     /locus_tag="ACHJ7J_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO5001722.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            8670..9389
                     /gene="rstA"
                     /locus_tag="ACHJ7J_003538"
     CDS             8670..9389
                     /gene="rstA"
                     /locus_tag="ACHJ7J_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO5001723.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEKTILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            9393..10694
                     /gene="rstB"
                     /locus_tag="ACHJ7J_003539"
     CDS             9393..10694
                     /gene="rstB"
                     /locus_tag="ACHJ7J_003539"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO5001724.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            10770..11699
                     /gene="tus"
                     /locus_tag="ACHJ7J_003540"
     CDS             10770..11699
                     /gene="tus"
                     /locus_tag="ACHJ7J_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO5001725.1"
                     /translation="MARHDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(11696..13099)
                     /gene="fumC"
                     /locus_tag="ACHJ7J_003541"
     CDS             complement(11696..13099)
                     /gene="fumC"
                     /locus_tag="ACHJ7J_003541"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO5001726.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGMTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(13242..14888)
                     /gene="fumB"
                     /locus_tag="ACHJ7J_003542"
     CDS             complement(13242..14888)
                     /gene="fumB"
                     /locus_tag="ACHJ7J_003542"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5001727.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKYGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            15087..16262
                     /gene="manA"
                     /locus_tag="ACHJ7J_003543"
     CDS             15087..16262
                     /gene="manA"
                     /locus_tag="ACHJ7J_003543"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO5001728.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIL
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            16363..17871
                     /locus_tag="ACHJ7J_003544"
     CDS             16363..17871
                     /locus_tag="ACHJ7J_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5001729.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEEMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(17916..19181)
                     /gene="uidC"
                     /locus_tag="ACHJ7J_003545"
     CDS             complement(17916..19181)
                     /gene="uidC"
                     /locus_tag="ACHJ7J_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO5001730.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         4734 a         4972 c         4639 g         4852 t
ORIGIN      
        1 ataaatatac attgtccttc tcctgcaaga gaattatttt aattttcgct taattcagcg
       61 aaaccaaagt aaagtggtca agccccgcgc ttagcgcaag ctttggcaga agaaaggact
      121 aaagcgcgtt gcgccagtgc tgctcaccta cgagcaagat agtggatgag gtacgaagat
      181 gggtaaatgt agaaaataac gtcctgaaca aattgtccat aatattacaa ttatccgcag
      241 tgttgcttct cgtcatcgcg gatgataatt gtcctcggta gttgaacacg cctgatttgt
      301 atcatagctt aagaattaac tcaaaatatt ttcatttctt tacctgagcg gtttgatttt
      361 cgttatgatg acggagcgaa aaagacatta ttattagcaa aggaagaaaa aacggggaca
      421 agcatggcaa agccgatcat cacgctcaat ggcctaaaaa tcgtcattat gttgggaatg
      481 ctggtcatta ttctctgcgg tatccgtttt gccgccgaga tcatcgtgcc gtttattctc
      541 gcattattta ttgctgttat tcttaacccg ctggtgcaac acatggtccg ctggcgagtg
      601 ccgcgtgtac tggcggtgtc gattttgatg accatcatcg tgatggcgat ggtgttgctg
      661 ttagcttatc tgggttccgc gctcaacgag ttgacgcgga cgttaccgca atatcgcaac
      721 tctattatga cgccgctgca agctcttgaa ccgttgttgc aacgcgtagg gattgacgtc
      781 tcagttgacc aactggcgca ttacattgat ccgaacgcgg cgatgacgtt gctcaccaac
      841 ttattgacgc agttatctaa tgccatgtca tcaatatttt tattgctgct gacggtgctg
      901 tttatgctgc tcgaagtgcc acaattgccc ggaaaatttc agcaaatgat ggcgcgtccg
      961 gttgaaggga tggcggcgat tcagcgtgcg attgacagtg tgtctcatta tctggtgctg
     1021 aaaacagcca tcagcatcat caccggcctg gtcgcatggg cgatgctcgc cgccctcgat
     1081 gttcgcttcg cttttgtctg gggattgctg gcctttgcgc ttaattacat ccctaatatt
     1141 ggttcagttc tcgcagcaat tccccctatc gctcaggtac tggtgtttaa tggcttctac
     1201 gaagcgttgt tggtgctggc gggatatctg ctgattaatc tggtcttcgg caatattctg
     1261 gagccacgca tcatggggcg tgggctggga ctttccacat tggtggtatt tttgtcgttg
     1321 attttttggg gatggttgtt aggaccggtg ggtatgctgc tttccgtgcc gttaacaatt
     1381 attgtcaaaa ttgcgcttga acaaacagcg ggaggtcaaa gcatcgccgt tctgttaagc
     1441 gatctcaata aagagtgacg gcctccgcag aggccgtcag ggttacagag ctttcaggat
     1501 tgcatccacg ctggctttgg cgtcaccaaa cagcatgtgg gtgttttcct tgaagaacag
     1561 cgggttttgc acaccagcat agccagtgtt catcgaacgt ttaaagacaa tcacgttctg
     1621 cgctttccac acttccagca caggcatacc agcaatcgga ctcttcggat catcctgcgc
     1681 cgccgggtta accgtatcgt tagcaccaat caccagtacg gtatcggtat cagcaaagtc
     1741 atcgttgatc tcgtccattt ccagcacgat gtcatacggt acttttgctt cagccagcaa
     1801 tacgttcata tgtccaggca aacgccccgc aaccgggtgg ataccgaaac gcacgttgat
     1861 accacgagcg cgcagtttct cggtaatttc agcgacagga tattgcgcct gcgcgactgc
     1921 catgccgtac cccggagtaa tgatcactga atgggagttt ttcagcagtt ccgctgtctc
     1981 ttctgcggtg atttcgcggt gctcacccac ttcctgatca tcgccagtag aagagccgtc
     2041 ggtgccgaaa ccacccgcaa taacgctgat aaaggaacgg ttcatcgcct tacacataat
     2101 gtaagaaagg atagcccccg aggaaccgac cagcgcaccg gtcacaatca gcaggtcgtt
     2161 gctgagcata aagcccgcag ccgcagccgc ccagccggag tacgagttca gcatcgacac
     2221 caccaccggc atatctgcac caccgatgga ggcgactaaa tgccagccaa ataccagcgc
     2281 aatggcggtc attatcagca atgccagcac ttgcaggccg acgctgtccg tgcgcacaaa
     2341 tacaatcagc agcaggaagg aaacgaccag agccgccagg ttcattttat gacggtttgg
     2401 cagcatcaac ggtttagacg aaatcttgcc acacagtttg ccgaacgcca ccaccgaacc
     2461 cgtgaacgtt accgccccga tgaagatacc gaggaacact tccgtcaggt gaatattgac
     2521 cagaatcggt gccattcccg cgtcatgatg cagatagctg ttaaagccaa ccagcactgc
     2581 cgccagaccc acgaagctat gcaggatcgc caccagttca ggcatttcgg tcatttcaac
     2641 tttcttcgcc agccggatac caattgcccc accaatgacc atcgccagca agatccagcc
     2701 aacattaccc gtatccggtc caaaaatggt ggcgattaac gcaatcgcca tcccggcgat
     2761 accgaagttg ttaccctggc gagacgtttc atgtttcgaa agaccggcca gactgaagat
     2821 aaacaggatc gcggcaacaa tgtatgcagc tgtaactaat cctccagaca tatgttaccc
     2881 cttaattttt gcggaacatt ttcagcatgc gctgagtcac ggtgaagcca ccgaaaatat
     2941 taatgctggc tataagcacc gcgataaaac taaggaagct aacccagccg ccctggccaa
     3001 tctgcaacag tgctccgaca acaataatcc ctgaaatcgc gttggtgacc gacatcaacg
     3061 gtgtatgcag cgcgtgcgat acattccaca ccacgtaata accgacaacg caggccagcg
     3121 cgaacacagt gaagtgccca agaaattctt tcggcgcaac gcttgccatc cagccaaaaa
     3181 gaatgattgc cagcgccatc aacgcgtatt tacgccacgg tgagcaggta catttttcct
     3241 cagttttcac ttccggtgcc gctttttgtg ccgcctgcgg ctgagctgat acctgaatcg
     3301 gcggtgccgg ccaggtaatt tcgcccgcac ggatcacggt cacgccgcga atcaccacat
     3361 catcaaaatc aacagtgata ttgccgtctt tctctttgca caacagtttc agcagattaa
     3421 cgaggttagt accgtaaagc tgtgaggatt gcgtcggcag acggcccgga agatcggtat
     3481 agccaatcac tttgacacca ttttccgtag tgaagatttc acccggcacg gtgtattcac
     3541 agttgccgcc gttttgggct gccaggtcga caatcacact gcccgccttc atggagtcaa
     3601 ccatttcacg ggtaattagc ttcggcgctg gtttgcctgg aataagcgcg gtggtgacaa
     3661 tgatatcgac ctcttttgcc tgggcggcaa agagttccat ttccgctttg atgaacgcgt
     3721 ccgacatcac tttggcatag ccatcgccgc tacccgcttc ctctttaaaa tccagctcga
     3781 ggaattccgc gcccatactt tgaacttgtt ctttcacttc cgggcgggtg tcgaatgcac
     3841 gcacaatcgc gccgagactg tttgctgcgc caatggcggc cagacctgca acacccgcac
     3901 caatcaccat cacttttgcc ggtggcactt tcccggccgc agtgatttgc ccggtaaaga
     3961 agcgcccaaa ttcatgtgcc gcttcaacaa tggcgcgata accggcgatg ttcgccatcg
     4021 agcttaatgc gtccagcgat tgtgcgcgtg agatacgcgg cacagagtcc atcgccatca
     4081 cggtcacgtt acgttccgca agtttttgca ttaattccgg attctgcgca ggccagataa
     4141 aactcaccag cgttgtccca ggattcagta acgcaatttc atcatctaac ggcgcattga
     4201 ccttcagaat gatctctgac tgccagacgc tattcccttc tacaatttca gcgcccgctt
     4261 gcacaaacgc tttatcgtca aaacttgcca gttgacccgc gccgctctct accgcgacgg
     4321 taaaacccag tttcagcagc tgttccactg tttttggcgt tgctgcaaca cgggtttcat
     4381 tggttaaccg ttctcttggt atgccaattc gcatgatatt cccttccatc ggttttattg
     4441 atgatggttt gcctgtgtca ggagccacac aagccgctca tgtacgagct aaatgttact
     4501 ccgttaaaat aaattagtaa caaacgccct ataacgtact gaaaattatg cctgtgatct
     4561 agcgccaaaa atcagtattt cggcgtgaac tcgcaaaata ttaacgattc agccgtgata
     4621 gtgggataaa caccttagaa cgccggataa agactgataa ttgtcttcga cggtcgggta
     4681 aaacgagaca tcgccccggc acgaatcact acttaacatt aaattaactt atacaattca
     4741 gttgcttcag tagtaatgat gctgatacgg ctgtttttta agcatagacg gtcatttgag
     4801 caggattaaa aatggcttaa ggaatgtgat atgaaaaatg acgcagacag ttacaccgtt
     4861 taaatgcaat aatcagccac gtttctcgtt aataacaata ccagtacctg gtttgcgcaa
     4921 ggcgaaggat tatttttatg aagcttaaga acaccctcct ggcgtcggca ctgctttctg
     4981 ctacggcatt ctccgttaac gcagcaacag aactgacacc ggagcaagcg gcagcggtta
     5041 aaccttttga ccgtgtagtg gttaccggtc gttttaatgc tattggcgaa gcggtgaaag
     5101 ccgtttctcg tcgcgcagat aaagaaggtg ccgcctcttt ttatgttgtc gacacttctg
     5161 attttggtaa cagcggtaac tggcgtgtgg tcgctgacct ctataaagcc gatgctgaaa
     5221 aagcagaaga aacaagtaat cgcgtaatta acggtgttgt cgaactgccg aaagatcagg
     5281 ctgttctgat tgaaccgttt gacacggtca ccgtccaggg cttctatcgt agccagccag
     5341 aagtcaatga tgccatcacc aaagcggcaa aagcgaaagg tgcctactct ttctacatcg
     5401 ttcgtcaaat cgatgccaac cagggcggca accagcgtat tactgcattc atctataaaa
     5461 aagatgctaa gaaacgtatc gtccagagcc cggatgtgat cccggcagat tccgaagcag
     5521 gacgtgcagc tctggctgcc ggtggcgaag ccgcgaagaa agttgagatc ccgggtgttg
     5581 cgactaccgc atcaccaagt tctgaagtcg gtcgcttctt tgaaacccag tcatcaaaag
     5641 gcgggcgtta caccgtcacg ctcccggatg gcactaaagt cgaagaactg aacaaagcga
     5701 ccgcagcgat gatggtcccg ttcgacagca tcaaattctc tggcaactac ggcaacatga
     5761 ccgaagtctc ctatcaggtt gcgaaacgtg ccgcgaagaa aggtgctaag tattaccaca
     5821 tcacccgcca gtggcaggaa cgtggtaata acctgaccgt cagcgcagat ctgtataaat
     5881 aactattcag ttactcctat aaggcggctt gatgccgcct ttcgcatttt tagtcacaaa
     5941 tttccctgcg accattgcat gcagacacat ctcttcgtaa aatcccgcgc ttcagtggca
     6001 aactccattt ttatgcgttt tgccctaata tttattcttc ttatcacgtt ttaatcactg
     6061 gatatcgatg gaaaagaaac tgggactgag cgcactcacc gcgctggtat taagctcaat
     6121 gctgggcgcg ggtgttttca gtctgccgca aaatatggcg gcagttgcca gcccggcagc
     6181 actgctcatc ggctggggta ttactggcgc tggcatttta ttgctggcct ttgccatgct
     6241 gatcctcacg cgcattcgtc ccgaacttga cggcggtatc tttacctatg cccgcgaagg
     6301 gtttggcgag ctaatcggct tttgttccgc atggggatac tggctgtgcg cagtcatcgc
     6361 caacgtctcc tatctggtga tcgttttttc cgcgttaagc ttttttacgg acacgccgga
     6421 attgcgcctg tttggcgatg gtaatacctg gcaatcgata gtcggtgcat cggctttatt
     6481 gtggattgtt cactttttga tcctgcgcgg ggtgcaaacc gctgccagca ttaacctggt
     6541 ggcgacactg gcaaaattgt tgccgctggg tctgtttgtt gtgctggcga tgatgatgtt
     6601 caaactggat accttcaagc tcgacttcac cggacttgca cttggcgtac ccgtttggga
     6661 acaggtgaaa aacaccatgc tgatcaccct gtgggttttc attggtgtgg aaggtgccgt
     6721 ggttgtttct gcgcgtgcgc gtaataaacg tgatgttggc aaagcgacac tgctggcggt
     6781 tctctccgct ctgggcgttt acttgctggt aacgctgctt tcgctgggag tggtggcacg
     6841 tcctgaactg gctgaaattc gtaacccgtc aatggccggt ctgatggtgg aaatgatggg
     6901 accgtggggc gaaatcatca tcgctgccgg tttgatcgtt tccgtttgcg gcgcgtactt
     6961 aagctggacc atcatggcag cagaagttcc gttcctggcc gccactcata aagcattccc
     7021 acgcattttt gcgcgccaga acgcacaagc tgcgccatcc gcctcactgt ggttgaccaa
     7081 catctgcgtg caaatttgtc tggtactcat ctggctgacc ggttccgatt acaacacgtt
     7141 gctgaccatc gcctcagaaa tgattctggt gccctatttc ctggtcggcg cgttcttgct
     7201 gaaaatcgca acccgtccac tgcataaagc ggtaggtgtc ggtgcctgca tttatggctt
     7261 atggttattg tacgcctctg gtccaatgca cttgctgtta tccgttgttc tgtatgcacc
     7321 ggggctgctg gttttcctgt atgcgcgcaa aacgcataca catgataatg tactgaaccg
     7381 tcaggaaatg gtgctgattg gcatgctgtt aattgcctca gttccggcga cctggatgct
     7441 ggtgggataa cgttcatccc atcgttggat agcaaaggag ataacgatgg gtaaaaccca
     7501 gcccttgcca atattaatta ctggcggagg tcgtcgcatc ggcctcgccc tcgcatggca
     7561 tttcattaat caaaagcaac ctgtgattgt cagctatcgg acacactatc cagccattga
     7621 tggactgatt aatgcaggtg cgcagtgtat tcaggctgat ttttcgacca acgacggtgt
     7681 gatggcgttt gccgatgaag tactaaaaag cacccatggt ctgcgtgcta ttttgcataa
     7741 cgccagtgcg tggatggcgg aaaaaccggg tgcgccactg gccgacgtac tggcttgcat
     7801 gatgcagatc cacgttaata ccccatacct gctcaaccat gcgctggaaa gattactgcg
     7861 tgggcacgga cacgccgcca gcgatatcat tcactttacc gattatgtgg tggagcgcgg
     7921 tagcgacaaa catattgcgt atgctgcaag caaagcggca ctggataata tgacccgctc
     7981 gtttgcccgc aagctggcac cggaagtgaa agtgaattct attgcgccat cgctgatcct
     8041 gtttaatgaa catgatgatg ccgaatatcg acaacaggcg ctgaataaat cattgatgaa
     8101 aaccgcgcct ggcgagaaag aagtgatcga cctggtcgat tacttactta ccagttgctt
     8161 tgtcaccgga cgcagtttcc cacttgatgg cggtcgtcat ctgcgttaat gcagctttat
     8221 ccagcatata atcaacaccc atgcgcttat cccccaacac gcaacagaac caacaaaagc
     8281 ggcgggcaag cgcatcattc cggtgaaata ccacagcgac accaggtaaa caaaataggg
     8341 aataatcgac cacatactaa aaatgatggt tgcgcgtaag gcttcaatgc cgcgttcgct
     8401 ggcaacaata taatgcgcga taagcgcaaa ggtcgggaaa agtggaatca gcccggcgat
     8461 ataataattt ttcgtttttg ctaaaacacc aatcaacagc actaccagcg caccgagcgc
     8521 ggctttgatt accagcccca tctttttacc ttaacacttc cataacaagt catcagtaga
     8581 atacctgatg aaaacttgtt tagaaacgat tgatagtaag taaaaacagc gcggtgtatt
     8641 gtgacgtttt tatatctacc gtgaatgtta tgaacactat cgtatttgtg gaagatgatg
     8701 cggaagtcgg ttcactgatt gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag
     8761 agccgcgcgg cgaccaggcc gaaaaaacca ttttgcgaga aaatccggat ttggtgttac
     8821 tcgacatcat gctaccaggc aaggacggca tgaccatttg tcgtgattta cgcgcaaagt
     8881 ggtctggacc gattgttctt ctaacctctc tcgatagcga tatgaaccac atcctggcac
     8941 tggaaatggg tgcctgcgac tatattctca aaacgacgcc ccctgctgtt ttgctagcgc
     9001 gtttacgttt gcatttgcgt cagaatgagc aagccacact gaccaaaggt cttcaggaaa
     9061 cgtctctgac tccctacaaa gccctgcatt tcggcacgtt gaccatcgat cccatcaacc
     9121 gcgtagtcac cctggctaac actgaaatct cgctctcgac agctgatttc gaattattgt
     9181 gggaattagc tacccatgcc gggcaaatca tggaccgcga tgcattgctg aaaaatttac
     9241 gcggcgtcag ttatgacggc ctggatcgta gcgtggacgt ggctatttcg cggttaagaa
     9301 aaaaactgct cgataacgcc gcagaacctt atcgcattaa aactgtgcgt aacaaaggct
     9361 atctctttgc gcctcatgca tgggaataag cgatgaaaaa actgtttatc cagttttacc
     9421 tattattgtt tgtctgcttc cttgtaatgt ctctgctggt tgggctggtg tacaaattta
     9481 ccgccgaacg cgcgggcaaa cagtcgctgg atgatttgat gaacagttcg ctgtatctga
     9541 tgcgcagcga attgcgtgag atccccccac acgactgggg taagacgctg aaagagatgg
     9601 atttaaatct ctctttcgat ctgcgtgtcg agccactgag taaataccat cttgatgata
     9661 tttccatgca ccgactgcgt ggcggcgaaa ttgtcgccct ggacgatcag tacacgtttt
     9721 tgcagcgtat cccgcgcagc cactacgtgc tggcagttgg tcctgttcct tatctttatt
     9781 acctccatca gatgcgattg ctggatatcg ccctgatcgc ttttattgct atttccctcg
     9841 cctttccggt gtttatctgg atgcgtccgc actggcagga tatgttaaaa ctggaagcag
     9901 cggcgcaacg atttggcgat gggcatctca atgaacgtat ccactttgat gagggttcga
     9961 gctttgaacg acttggtatc gcatttaacc agatggcgga caatatcaac gccttgattg
    10021 ccagcaaaaa acagcttatt gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc
    10081 tgcgttatcg actggagatg agcgataacc tgagcgccgc cgaatcccag gcgttgaatc
    10141 gtgatatcag tcaacttgaa gctttaattg aagagctgct gacttatgcc cgactcgatc
    10201 gcccacaaaa cgagcttcat cttagcgaac cagacctgcc gttgtggcta tcaacgcatc
    10261 tggcagatat tcaggcagta acgcccgata aaacggtacg gataaaaacg ctcatgcaag
    10321 gccattatgc ggcgttggat atgcgcttaa tggagcgcgt gctggataat ttgctcaata
    10381 acgccctgcg ctactgccat tcaacggttg aaaccagcct gctactgtcg gggaatagag
    10441 cgacattaat tgttgaggat gatggcccag ggattgcccc agaaaaccgc gaacatatct
    10501 ttgaaccttt tgttcgcctc gatcccagcc gggatcgctc aaccggcggc tgcgggctgg
    10561 ggctggcaat tgtccactct atagcactgg caatgggcgg tacggttaat tgtgacacca
    10621 gcgaactggg tggtgcccgc ttctcgttta gctggccgtt atggcataac atcccgcaat
    10681 ttacctctgc ttgacactac gcgcacggtg gtcaagtcac caccactgtg ctataaatta
    10741 aggatgttgt aactaaagtg gttaatatta tggcgcgtca cgatctcgta gaccgactca
    10801 acactacttt tcgccagatg gaacaagagc tggctgcatt tgccgctcat cttgagcaac
    10861 acaagctatt ggttgcccgc gtgttctctt tgccggaggt aaaaaaagag gatgagcata
    10921 atccgcttaa tcgtattgag gtgaaacaac atctcggcaa cgacgcgcag tcgctggcgt
    10981 tgcgtcattt ccgtcattta tttattcaac aacagtccga aaatcgcagc agcaaagccg
    11041 ctgtccgtct gcctggcgtg ttgtgttacc aggtcgataa cctttcgcaa gcagcgttgg
    11101 tcagtcatat tcagcacatc aataaactca agaccacgtt cgagcatatc gtcacggttg
    11161 aatcagaact ccccaccgcg gcacgttttg aatgggtgca tcgtcatttg ccggggctga
    11221 tcacccttaa tgcttaccgc acgctcaccg ttctgcacga ccccgccact ttacgttttg
    11281 gctgggctaa taaacatatc attaaaaatt tgcatcgtga tgaagtcctg gcacagctgg
    11341 aaaaaagcct gaaatcacca cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa
    11401 aactggagcg agagtatcag gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca
    11461 aacgtccggt gaaggtgcag ccgattgccc gcgtctggta caaaggagat caaaaacaag
    11521 tgcaacacgc ctgccctaca ccgctgattg cactgattaa tcgggataat ggtgcgggcg
    11581 tgccggacgt tggtgagttg ttaaattacg atgccgacaa tgtgcagcat cgttataaac
    11641 ctcaggcgca gccgcttcgt ttgatcattc cacggctgca cctgtatgtt gcagattaac
    11701 gcccggcttt catactgccg accatctgtt ctggccgtac ccagctgtca aactcggctt
    11761 cgctaagata ccccagcgca agggccgcag cttttaaggt catcccttct ttatgcgctt
    11821 ttttggcgat ctccgcggct ttgtcataac caatgtgggt gttaagcgca gtcaccagca
    11881 tcagcgattc attgagtaat tgattgattc gctcacgatt cggttcaata cccactgcgc
    11941 agtgtttgtt aaaactttcc atgccatctg ccagcaagcg caccgattgc aggaaattat
    12001 ggatcaccat cggacggaag acgttcagtt caaagttacc ggaagcgcca cccatgttga
    12061 tcgccacgtc gttccccatc acctgacagc agagcatggt taatgcctca cactgtgttg
    12121 ggttcacttt ccctggcatg attgagctgc ccggctcatt ttccgggatt gagatttcac
    12181 caattccgca gcgcgggccg gaggccagcc agcggacatc attggcgatt ttcatcagtg
    12241 acgcagccaa ccctttcaac gcgccgtgcg cctgaaccag ggcatcacag gtcgccagcg
    12301 cttcaaattt gttcggcgcg gtaacaaacg gtgcacaggt aatgactgcc agttcatctg
    12361 ctacgcgacg cgcatactcc ggatgggtat ttagtccagt acccaccgct gtaccgccaa
    12421 gagccagttc cgctacgtga ggcaggctgt attcgatatg tttgagatta tgctcgagca
    12481 tcgctaccca gccggaaatc tcctgcccca gcgttaacgg cgtggcatcc tgcaagtgag
    12541 tacgaccaat tttgacgata tcggcaaatg cacgggattt ctcattcagt gtctgtgtca
    12601 gggttttaag ctgcggaatg agttgcttgc gcagcgccag cagcgccgca acgtgcatcg
    12661 ccgtcggaaa gacatcgttg gaactttggc ttttgttcac gtcgtcgtta gggtgaactt
    12721 tacgttccat cccgcgcacg ccgccgagta attcactggc ccggttagcc agcacttcgt
    12781 tcatgttcat gttactttgc gtgccggagc cggtctgcca gatagccagc gggaattcgt
    12841 cgtcatgctg tcctgctagt acttcatccg ccgcctgccg aatggcgctc gctttctctt
    12901 cagacaacaa gcctaaatct tcattaactt ttgccgctgc gcgcttggtt agcgccagcg
    12961 catgaatcag tgaggtgggc attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt
    13021 gagtttgtgc gccccacagc ttatctgccg ggacatcaat cgcccccatc gaatcttttt
    13081 cgctgcgtac tgtattcatg gcctgctcct cacctgatta attttttctt tctgttttgc
    13141 tttcgttaag caacttttag ctcacttatt atttaccatt tgataacaaa tgtttggtct
    13201 ttcgtgccat gtaaaaaaac cgccccgaag ggcggctctg tttatttcac acagcgggtg
    13261 cattgtgtga gttgtatctg ctggaagaag tcatttcctt tatcatccac aaggataaac
    13321 gccgggaaat cttccacttc aattttccag atggcttcca ttcccagttc cggatattca
    13381 acacattcca ggctcttaat acttccctgc gccaatacag cggccggacc accgatactg
    13441 ccaaggtaga agccgccgta ttttttacag gcatccgtca cctgctggct gcggttgcct
    13501 ttcgccagca tgatcatact tccgccctgc gcttgcagtt gatcgacata agaatccatc
    13561 cgtccggcgg tcgttgggcc aagagaaccg gaggcataac cttccggcgt tttggccgga
    13621 cccgcgtagt aaatcggatg atctttaatg tactgcggca gcccttcacc gttatccatc
    13681 cgctctttca gtttggcgtg ggcaatatca cgaccaacga taatcgtgcc gttaagcgat
    13741 aagcgtgtag aaacgggata ctgcgacaac tgtgcgagga tctctttcat cggacggtta
    13801 aggtcaacgc gcaccgcttc gccttctccc gctttgcgca gctcttccgg gatatatttg
    13861 cctggattat gttccagttt ttcgatccag atcccctgac ggttgatctt cgctttgata
    13921 ttacggtcag cagagcagga gacgcccata ccgaccgggc aggatgcgcc gtgacgtggc
    13981 aggcgaatca cgcggatgtc gtgagcgaag tatttaccac caaactgcgc acccagacca
    14041 agattttgcg cttcgatcag caattctttt tccagttcca catcgcggaa cgcctgaccg
    14101 tgctcattcc cttccgttgg cagttcatca tagtatttcg cggaagccag tttcaccgtt
    14161 ttaaggttcg tttctgcaga agttccacca ataacgaacg caatatgata cggaggacag
    14221 gccgccgtac ccagcgtgcg catcttctca accaggtaat ttttcagttt ccccggcgtc
    14281 agtaacgctt tggtttcctg atagagatac gtcttgtttg ccgaaccacc acctttggcg
    14341 atacagagga atttgtactc gtcgccatca acggcataaa gatcgatctg cgctggtaga
    14401 ttggtgccgg tattcacttc tttatacata tccagcggcg cgttttgcga gtagcgcaga
    14461 ttatcttcga tataagtgtt atagacaccg cgcgccagcg ccgcttcatc accaccaccg
    14521 gtccatacac gctgcccttt tttaccaaca ataatcgcgg tgccggtatc ctgacaggtt
    14581 ggcagaacgc ctttcgccgc gatgtcggag ttacgcagga attgcagcgc cacatattta
    14641 tcattttcgc tggcctccgg gtcacgcaga atgtcggcca cctgttgttg gtgcgccgga
    14701 cgcagcataa acgacgcatc atgaaacgcc tgacgcgcca acagagttaa cgcttcgggt
    14761 gcgactttca aaatctcctg cccttcaaat tcagatacgc taacgtgttc gctggttagc
    14821 aggtaatact cagtatcatc ttttttgagt ggaaaaggag cctgataatg aaagggtttg
    14881 tttgacattg ttctctcact tactgcctgg tttggttatg ctctgggcgg gtgttccgtt
    14941 gccctgttaa aagcgagtaa caatatccta cacacttttt taacaaaaac tgagactagt
    15001 acgacttttt gcggctccag gttacttccc gtaggattct tgctttaata gcgggattaa
    15061 tttccacatt aaaacaggga ttgatcatgc aaaaactcat taactcagtg caaaactatg
    15121 cctggggcag caaaacggcg ttgactgaac tttatggtat ggaaaatccg tccagccagc
    15181 cgatggccga gctgtggatg ggcgcacatc cgaaaagcag ttcacgagtg cagaatgccg
    15241 ccggagatat cgtttcactg cgtgatgtga ttgagagtga taaatcgact ctgctcggag
    15301 aggccgttgc caaacgcttt ggcgaactgc ctttcctgtt caaagtatta tgcgcagcac
    15361 agccgctctc cattcaggtt catccaaaca aacacaattc tgaaatcggt tttgccaaag
    15421 aaaatgccgc aggtatcccg atggatgccg ccgagcgtaa ctataaagat cctaaccaca
    15481 agcctgagct ggtttttgcg ctgacgcctt tccttgcgat gaacgcgttt cgtgaatttt
    15541 ccgagattgt ctccctactc cagccggtcg caggtgcaca tccggcgatt gctcactttt
    15601 tacaacagcc tgatgccgaa cgtttaagcg aactgttcgc cagcctgttg aatatgcagg
    15661 gtgaagaaaa atcccgcgcg ctggcgattt taaaatcggc gctcgacagt cagcagggtg
    15721 aaccgtggca aacgattctt ttaatttctg aattttaccc ggatgacagc ggcctgttct
    15781 ctccgctgtt gctgaatgtg gtgaaattga atcctggcga agcgatgttc ctgttcgctg
    15841 aaacaccgca cgcttacctg caaggcgtgg cgctggaagt gatggcaaac tccgataacg
    15901 tgctgcgtgc gggtctgacg cctaaataca ttgatattcc ggaactggtt gccaatgtga
    15961 agttcgaagc caaaccggct aaccagttgt tgacccagcc ggtgaaacaa ggtgcagaac
    16021 tggacttccc gattcctgtt gatgattttg ccttctcgct gcatgacctt agtgataaag
    16081 aaaccaccat tagccagcag agtgccgcca ttttgttctg cgtcgaaggc gatgcaacgt
    16141 tgtggaaagg ttctcagcag ttacagctta aaccgggtga atcagcgttt attgccgcca
    16201 acgaatcacc ggtgactgtc aaaggccacg gccgtttagc gcgtgtttac aacaagctgt
    16261 aagagcttac tgaaaaaatt aacatctctt gctaagctta ttaaaggctt ataacacctt
    16321 caggcggcca gtccgcctga tttcatttta tggataatca ttatgaataa atcgctggta
    16381 gcggtaggcg tcattgttgc gctaggcgta gtctggacag gcggcgcatg gtatacaggc
    16441 aagaagattg aaacccatct cgaagagatg gtcgcgcagg cgaacgcgca actcaaactg
    16501 acagctcctg aatccaacct ggaagtgagt tatcaaaact atcatcgcgg cgtattcagc
    16561 agccagttgc aactgttggt gaaacccatt gccgggaaag aaaatccgtg gattaaaagc
    16621 ggtcagagcg tcatcttcaa cgaatcggtt gatcatggtc ccttccccct tgcccagctt
    16681 aaaaaactga acctgatccc atcgatggca tcaattcaaa ccacgctggt taataacgaa
    16741 gtaagcaaac cactgtttga tatggcaaaa ggtgaaacgc cttttgagat taactcgcgc
    16801 attggttaca gcggtgattc cagttccgat atttcgctca agccgctgaa ttacgagcaa
    16861 aaggatgaaa aagtcgcctt tagcggcggc gagttccagt taaatgcgga cagagacggc
    16921 aaagctatct ccctttccgg ggaggcgcaa agtggtcgga tagacgcggt taacgaatac
    16981 aaccagaaag tacagttgac ctttaataat ctgaaaaccg acggttccag cacgctggca
    17041 agttttggtg agcgcgtagg aaaccaaaaa ctgtcactgg aaaaaatgac catttcagtg
    17101 gaaggcaaag aactggcact gctggaaggc atggagatca gcggtaaatc ggatctggtc
    17161 aatgacggta aaacgatcaa tagccaactg gattactcgc taaacagcct gaaggtacag
    17221 aatcaggatc tgggcagcgg caagctgact ttaaaagtcg gccagattga tggtgaagcc
    17281 tggcatcagt ttagccagca atataacgcg caaactcagg cgctgctggc acagccagaa
    17341 attgccaaca atcccgaact ttatcaggag aaagtgacgg aagccttctt tagcgccctg
    17401 ccgctgatgt tgaaaggcga tccggtgatt actatcgcgc cgctaagctg gaaaaacagt
    17461 cagggtgaaa gtgcgctgaa tctgtcgctg ttcctgaaag acccggcaac gactaaagaa
    17521 gcgccgcaaa cgctggcgca ggaagtagat cgttcggtta aatctctgga tgcgaaactg
    17581 accattccgg tggatatggc aactgagttg atgactcagg tagcgaagct ggaaggttat
    17641 caggaagatc aagcgaaaaa actggcgaaa cagcaagttg aaggtgcatc agcaatgggg
    17701 cagatgttcc gtctgaccac cttgcaggac aataccatca ccaccagcct gcaatatgct
    17761 aacggtcaga taacgttaaa tgggcagaaa atgccactgg aagatttcgt tggtatgttt
    17821 gcaatgccgg cattaaacgt tccggtcgta cccgctattc cgcagcagta attcacagcc
    17881 aaaacgccgg aggtttttct ctccggcgtt atttcttact tcagcataaa gtcatactga
    17941 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    18001 tcccgccctt tatcggcagc aagaaagaaa ttcatcccct gtaacggccc ttcgctgacc
    18061 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    18121 tcatagccgc gagtcacaat cggtgcaaat gtcagcgtaa attgcggggt taaacgatag
    18181 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    18241 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    18301 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    18361 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    18421 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    18481 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    18541 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    18601 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    18661 cacattcccc acagatcaat atttggcgtt aaagcccaat aacctaatgc tccttcagaa
    18721 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    18781 ccgtaactat aatccgtccc gaagcgacct aatttaagca ggctattttc actgggtttt
    18841 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    18901 ctcatatcag cacgagcacc taatttataa acataatacg ctccgccttc aacgccaata
    18961 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    19021 tctcttcccg cgccagcact gggcttatca gccctgcgca attcgttttt cagtctgatg
    19081 cgtagtcccg cttcatcgtc agccagttgc gccgcgtaag cagaggtaag gccagaggca
    19141 gccgtcaggc aaataacggc catggccact atttttctca tagttgcacc tttgatg
//
LOCUS       DAZFFG010000090        18323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFFG010000090 DAZFFG010000000
VERSION     DAZFFG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.090"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1191)
                     /locus_tag="ACHJ7J_003546"
     CDS             complement(<1..1191)
                     /locus_tag="ACHJ7J_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5001731.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARKLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWATELSLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFCGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWAD"
     gene            complement(1378..1462)
                     /locus_tag="ACHJ7J_003547"
     tRNA            complement(1378..1462)
                     /locus_tag="ACHJ7J_003547"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1426..1428),aa:Leu,seq:caa)
     gene            1658..2677
                     /gene="ahr"
                     /locus_tag="ACHJ7J_003548"
     CDS             1658..2677
                     /gene="ahr"
                     /locus_tag="ACHJ7J_003548"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO5001732.1"
                     /translation="MSMIKSYAAKEAGGELELYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTILNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            complement(2681..3244)
                     /gene="idnK"
                     /locus_tag="ACHJ7J_003549"
     CDS             complement(2681..3244)
                     /gene="idnK"
                     /locus_tag="ACHJ7J_003549"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5001733.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            3461..4492
                     /gene="idnD"
                     /locus_tag="ACHJ7J_003550"
     CDS             3461..4492
                     /gene="idnD"
                     /locus_tag="ACHJ7J_003550"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO5001734.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKAEEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            4516..5280
                     /gene="idnO"
                     /locus_tag="ACHJ7J_003551"
     CDS             4516..5280
                     /gene="idnO"
                     /locus_tag="ACHJ7J_003551"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO5001735.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            5342..6661
                     /gene="idnT"
                     /locus_tag="ACHJ7J_003552"
     CDS             5342..6661
                     /gene="idnT"
                     /locus_tag="ACHJ7J_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO5001736.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6728..7726
                     /gene="idnR"
                     /locus_tag="ACHJ7J_003553"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6728..7726
                     /gene="idnR"
                     /locus_tag="ACHJ7J_003553"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO5001737.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7804..9306
                     /gene="yjgR"
                     /locus_tag="ACHJ7J_003554"
     CDS             7804..9306
                     /gene="yjgR"
                     /locus_tag="ACHJ7J_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO5001738.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGVLGSLLGGRKR"
     gene            complement(9432..10514)
                     /gene="lptG"
                     /locus_tag="ACHJ7J_003555"
     CDS             complement(9432..10514)
                     /gene="lptG"
                     /locus_tag="ACHJ7J_003555"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5001739.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(10514..11614)
                     /gene="lptF"
                     /locus_tag="ACHJ7J_003556"
     CDS             complement(10514..11614)
                     /gene="lptF"
                     /locus_tag="ACHJ7J_003556"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5001740.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(11677..11727)
                     /gene="ytgA"
                     /locus_tag="ACHJ7J_003557"
     CDS             complement(11677..11727)
                     /gene="ytgA"
                     /locus_tag="ACHJ7J_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO5001741.1"
                     /translation="MSIIILATISTTQENK"
     gene            11881..13392
                     /gene="pepA"
                     /locus_tag="ACHJ7J_003558"
     CDS             11881..13392
                     /gene="pepA"
                     /locus_tag="ACHJ7J_003558"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO5001742.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            13746..14189
                     /gene="holC"
                     /locus_tag="ACHJ7J_003559"
     CDS             13746..14189
                     /gene="holC"
                     /locus_tag="ACHJ7J_003559"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO5001743.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            14189..17044
                     /gene="valS"
                     /locus_tag="ACHJ7J_003560"
     CDS             14189..17044
                     /gene="valS"
                     /locus_tag="ACHJ7J_003560"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO5001744.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(17100..18296)
                     /gene="yjgN"
                     /locus_tag="ACHJ7J_003561"
     CDS             complement(17100..18296)
                     /gene="yjgN"
                     /locus_tag="ACHJ7J_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO5001745.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSNSVIISVVLMAIVGIVSIGVFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4679 a         4575 c         4667 g         4402 t
ORIGIN      
        1 atcggcccac cattgtagca tcaagcggcg ttcttccaga tgctctgctt tatggatata
       61 cgcggccctc actgaatttc gctccatgtg gctcatctga cgttcaacag catcacgaga
      121 ccacaaacct gattcgacca atgaactaca ggccattgtt cgaaagccat gaccacaaac
      181 ctctactttt gtatcatacc ccatgacccg taacgcacta tttaccgtat tctcactcat
      241 gggtttgtgc gaatcgtgat caccaataaa tatcaagtca tgggccccac aaaactgttt
      301 tatctgcttt aaaattgcaa gagcttgcgt tgaaagaggc actagatgcg ttgtacgcat
      361 ttttgagcct ctatgggaat gtttcactcc aggaataggc tcccgctccg gtgggatagt
      421 ccatatagac gcttcgaaat cgatctctga ccaacgagca aaacgcagct cactggaccg
      481 aataaagatc agcaaagaga gttctgtcgc ccatcgggtt agcggcctac cagtatagct
      541 atctattttt gtaagcaact cagggatgcg ctttaattca agcgcgggac gatgttgtcg
      601 attacaggaa gcaaccgccc cagccatctc ttgtgccggg ttataatcaa ttaacccact
      661 ttgcactgca tagcgcatga tggctgtagt gcgctgctga agacgagcgg ccacttcaag
      721 acgtccagac atttctacgg ccttaatggg tgctaataaa tctcgagttt ttaactcagc
      781 gatattacgt tcaccaagcg ctgcaaaaag attatcttcc agacttttta gcacacgatg
      841 ggcgtgatct tcagaccact ttttattggt gccatgccac tcaatcgcga cttctttaaa
      901 ggttcgtgct ttactctgtt caaccttatc atttttcttt ttgtctcccg gatcgacgcc
      961 attcgcaagc agcttacgcg cctcgtcacg acgtactctg gcatccgcta gtgtgatttc
     1021 aggataaacc ccaagtgcca gcattttttg ctttccctca taacggtact gcaaacgcca
     1081 gtacttagaa ccatttggat ggacaagcag atgcataccg ccaccatcag tcagcttata
     1141 ttgcttatcc gttggcttgg ctgctctgac tttgatatct gttaatgcca tgcgtattcc
     1201 ctccctgttg gtacaagcat tatcgaaccg gaaataccat catctgtacc aacatatgtt
     1261 ggtagatgta cgttgatatc ggttgacctt gagagagtta atatagcggg aaaggtagat
     1321 aaacactgga tttcaggcat aaaaaaagac ctcagttgag gtctatttac atacttttgg
     1381 tgccgaaggc cggactcgaa ccggcacgta tttctacggt tgattttgaa tcaactgcgt
     1441 ctaccgattt cgccacttcg gcactgaaga ggtatgcgga aaacgttgtg gattatacct
     1501 gtcgcacgcc accatgcaag cgccagcttg ctcgcacctc gctaagtgct gaaattttca
     1561 gcattgcata cagcgatgtg taacctttgt cacactccag gcacccctcc ctgccatgct
     1621 ctacacttcc cagacaacac cagagaagga ccaaaaaatg tcgatgataa aaagctatgc
     1681 cgcaaaagaa gcgggcggcg aactggaact ttatgagtac gatcccggtg aattgaagcc
     1741 acaagatgtt gaagtgcagg tggattactg cgggatctgc cattccgatc tgtcgatgat
     1801 cgacaacgaa tggggatttt cacaatatcc actggttgcc gggcatgagg tgattggtcg
     1861 cgtagtggcg ctcgggagtg ccgcgcagga taaaggtttg caggtcggtc agcgtgtcgg
     1921 gattggctgg acggcacgca gctgcgggca ttgcgatgcc tgtattagcg gtaatcagat
     1981 caactgcgag caaggtgcgg tgccgacgat tttgaatcgc ggcggttttg ccgagaagtt
     2041 gcgtgcagac tggcaatggg taatcccact gccagaaaat atcgacattg aatctgctgg
     2101 gccgctgttg tgcggcggta tcacggtctt taaaccactg ttgatgcacc atatcactgc
     2161 caccagccgc gtcggcgtga ttggtattgg cgggctgggg catatcgcta taaaacttct
     2221 gcacgcaatg ggatgcgagg tgacagcctt tagttctaat ccggcgaaag agcaggaagt
     2281 actggcgatg ggtgccgata aagtggtgaa tagccgcgat ccgcaggcac tgaaaactct
     2341 ggcggggcag tttgatctca ttatcaatac tgtgaacgtc agcctcgact ggcagcctta
     2401 ttttgaggcg ctgacctatg gcggtaattt ccatacggtc ggtgcggttc tcacgccgct
     2461 gtctgttccg gcctttacgt taattgcggg cgaccgcagc atctctggtt ctgctaccgg
     2521 cacgccttat gagctgcgaa agctgatgcg ctttgccgcc cgtagcaagg ttgcgccgac
     2581 caccgaactg ttcccgatgt cgaaaattaa cgacgccatc cagcatgtgc gcgacggtaa
     2641 ggcgcgttac cgcgtggtgt tgaaagccga tttttgaaaa tcattcgcag cgctgatctg
     2701 aggcgctgcc ctctttcgca catattctgt tttgtcgtat cgccagcaca gcctgccgac
     2761 attgttcggt gacattggca atatcatggt tgatatcaat gcgcacaata tcctgttcat
     2821 ctgcttgtgg acgctccagc gcctcaaact gactttttag taacgctacc ggcataaaat
     2881 gcccagcccg acgctgcatt cgcgcgagga tagtttcata gtcgccatct aaccagagga
     2941 aatgaacatg ggggctaccc ttgcgtaaaa tatcacgata ctgttttttt aatgatgaac
     3001 agacaataaa tcctgtttca ttctttttat aaagactgta tgaagcatca tttaagcgtt
     3061 ccagccaggg aagtcgatct tcatcagata atggaatacc ctgcgacatt ttatctatat
     3121 ttttggctgg atgaagatcg tcaccatcaa taaatttagc agataataac gcggcaacct
     3181 tgctaccaat taatgtttta ccactccctg aaacgcccat caaaataaag ctttcacccg
     3241 ccatttaaaa tctcctgaat catcttacct gctgcgatac tacgcctggt ggcataataa
     3301 tttcacgttt ttttatttct gctgataaga attacaaggc acatcacgtt atgcgtaaca
     3361 tagtaatgta acaattttct gacgtgatct tcatcacaaa taatgacaat taaaaccgct
     3421 taaatgcttc cgaggtgtct acctgaccag tgaggaattt atgcaagtga aaacacagtc
     3481 ctgcgttgtt gcgggcaaga aaactgttgc cgttaccgag cagacgatag attggaataa
     3541 taatggaaca ttagtacaaa taacccgagg tggaatttgc ggttccgatt tacattatta
     3601 tcaggaagga aaagtaggta atttcatgat aaaggcaccg atggtgttag gtcatgaagt
     3661 tatcggtaaa gttattcata gcgactcatc agaattacat gaagggcaaa cggtagccat
     3721 taatccgtct aaaccgtgcg gtcactgcaa atactgcatt gaacataacg agaatcagtg
     3781 tacagagatg cgtttttttg gcagtgccat gtatttccct catgttgatg gtggctttac
     3841 ccgttataaa atggtcgaaa cgtcgcaatg tgtcccttat ccggccaaag ctgaagaaaa
     3901 ggttatggct tttgccgaac ctttagccgt cgcgattcat gccgcacatc aggccggcga
     3961 gttacagggc aagcgagtat ttatttctgg tgttgggccc attggctgcc tgattgtcag
     4021 tgctgtgaaa acactggggg ccgcggaaat tgtctgtgcc gatgtgagtc cccgttccct
     4081 ttcgctgggc aaagagatgg gggcggatgt gctcgtaaac ccacaaaacg acgacatgga
     4141 tcactggaaa gcggaaaaag gctatttcga tgtcagcttt gaagtgtccg gtcatccttc
     4201 atcagtgaat acctgtctgg aggtcactcg tgcacgcggc gtaatggtgc aggtaggtat
     4261 gggaggcgcg atggcagaat tcccaatgat gacgttgatt ggtaaggaga tttcactcag
     4321 aggctctttc cgttttacca gcgaatttaa taccgcagtg tcatggctgg cgaatggcgt
     4381 tatcaatcca ctgcctttac tgagtgctga atatcccttc actgacctgg aagaggcgct
     4441 acgtttcgcc ggtgataaaa cccaggcagc aaaagtccag cttgttttct aagagataaa
     4501 taaaggaata atacaatgaa cgatctattt tcactggcag gaaaaaatat cttgattacc
     4561 ggttcagcac agggcattgg ctttttactg gcaaccggcc tgggtaaata tggcgcacaa
     4621 ataattatta atgatattac tgccgaacgc gcagaacttg ctgtagaaaa actccaccag
     4681 gagggtattc aggccgttgc cgcacctttt aatgttactc ataaacatga aattgatgcc
     4741 gccgttgaac atatcgaaaa ggacatcggc cccattgatg tgctggtgaa taacgccggt
     4801 atccagcgcc gtcatccttt tactgagttc cctgaacaag agtggaatga tgtgatcgca
     4861 gtaaaccaga ccgccgtgtt cctggtatcg caagcggtaa ctcgtcacat ggttgaacgc
     4921 aaggcaggta aagttattaa tatttgctcg atgcaaagcg aactgggacg tgacaccatc
     4981 accccttatg ccgcatcgaa aggggcggta aaaatgctca cccgcggcat gtgcgtcgag
     5041 ctggcgcgcc acaatattca ggtcaacggt attgcgccgg gctatttcaa aacagaaatg
     5101 actaaagcac tggttgagga cgaagccttc accgcctggt tgtgcaaacg gacccccgcc
     5161 gcacgctggg gagatccgca ggaactgatt ggtgctgcgg tgttcctttc ttcaaaagcc
     5221 tctgatttcg taaacggcca cctgttgttt gttgatggcg gcatgttagt ggctgtttaa
     5281 caacccccct acgtgcagcg cgaaagcgct gcttttctgg cactaataag agataaagac
     5341 tatgccatta atcattattg cggcaggcgt cgcgctgctt cttatcctga tgatcggctt
     5401 taaagttaac ggctttattg ccctcgttct ggtagctgcc gtcgtcggat ttgccgaagg
     5461 gatggatgca caggccgtcc tgcactctat acaaaatggt atcggcagca cgctcggcgg
     5521 gctggcaatg atcctcggtt tcggggccat gttaggcaag ctgatttctg atacgggtgc
     5581 ggcacaacgt atcgccacta cgctgattgc tacttttggt aaaaaacgcg tgcaatgggc
     5641 gctagtgatc accggtctgg ttgtgggcct cgccatgttt tttgaagtgg gttttgtcct
     5701 gctgttgccg ttggtattta ccatcgtagc atcatcagga ttacccctgt tgtatgttgg
     5761 cgtaccaatg gtagcagcgc tctctgtaac ccactgtttt ctgccgccac atccagggcc
     5821 tactgccatc gcgactatct ttgaggctaa tctcggaacg actttactgt atggatttat
     5881 cattaccatt ccgacagtta ttgtcgcagg accgctgttt tctaaactgc taactcgctt
     5941 tgagaaagca ccaccggaag gcttatttaa tcctcatctg tttagcgaag aggagatgcc
     6001 ctccttctgg aacagtattt tcgctgccgt gatcccggtc atcctgatgg ctatcgccgc
     6061 cgtttgtgaa attacgttac cgaaaactaa caccgtgcgc ctcttctttg aatttgtcgg
     6121 taaccctgcc gttgcgctgt ttattgccat tgttattgcg attttcacac tgggccgacg
     6181 taatggacgc accatcgagc aaatcatgga tatcattggg gattctatag gcgctatcgc
     6241 gatgattgtg tttattatcg ctggcggcgg cgcgtttaag caggtattag tagatagcgg
     6301 tgtcgggcag tatatttcac acttaatgac cggaactacg ctttcgccgt tattgatgtg
     6361 ctggactgtt gcggcgctgt tgcgtatcgc tctgggctct gccaccgtcg ctgccattac
     6421 caccgcgggt gtggtgttgc cgattatcaa cgttacccat gccgatcccg ctttaatggt
     6481 actggcaacc ggtgcgggca gtgtgatcgc gtcacacgta aacgaccctg gcttctggct
     6541 atttaaaggg tattttaatc tgacggttgg tgaaacgttg cgtacctgga cggtgatgga
     6601 aacccttatt tctattatgg gtttgctggg cgtgttagcc attaacgccg tattgcactg
     6661 aaagaataaa atattgtgat gtagtttcgg gggaagattg aagattcccc cgacgttggg
     6721 agaagttatg cgcaatcaca gaatttcttt acaggatatc gctacgctgg ctggcgtaac
     6781 aaaaatgacc gtgagtcgtt atatccgctc gccgaaaaag gtggcaaagg aaacaggcga
     6841 gcgcatcgcc aaaattatgg aagagatcaa ttatatccct aatcgcgctc ccggcatgtt
     6901 gttgaatgca caaagttata ccctcggtat cttgattccg tcatttcaaa atcaactctt
     6961 cgctgacatt ctcgcgggaa tagaatccgt cacctctgag cataattatc agacccttat
     7021 tgctaattac aattacgatc gcgattccga agaagagtcg gtgattaacc tgctctctta
     7081 taacattgac gggattattc tttcggaaaa atatcacacc atcaggacgg tgaaatttct
     7141 gcgttcagcc accattccgg tcgtggaatt gatggatgta cagggagaac ggctggatat
     7201 ggaggtcggt tttgataatc ggcaggccgc ttttgacatg gtgtgtacca tgctggagaa
     7261 gcgagtcagg cacaaaattc tgtatctcgg ttccaaagat gacacccgcg atgaacagcg
     7321 ttatcagggg tattgcgatg cgatgatgct gcataatctt tccccattac gcatgaatcc
     7381 acgcgccatc tcatccattc atttaggaat gcagttaatg cgtgatgccc ttagcgcaaa
     7441 cccggattta gacggtgtat tttgcaccaa tgacgatatt gcgatgggtg cgcttctgct
     7501 gtgccgcgag cgaaacctcg ccgtgccaga acaaatttcc attgcgggtt ttcatgggct
     7561 ggagattggc aggcaaatga tcccaagcct cgccagcgta attactcccc gttttgatat
     7621 tggtcggatg gccgcccaaa tgttgctgag caaaattaaa aataacgatc acaaccacaa
     7681 cactgttgac ctcgggtatc agatttatca cggcaacacg ctttaggtta ttttctcttc
     7741 attacacgta ttctctttgc cggacgttgc ctatactcca ggcaggaaac tggaggaaat
     7801 ctcatgagcg aacccctgtt aattgcccgc acgccggaca cagaactgtt tttactgccg
     7861 ggaatggcga accgtcacgg gctgattact ggcgcaacgg ggacgggtaa aaccgttacg
     7921 ctgcaaaaac tggcagagtc attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg
     7981 aaaggcgatc tgaccggtat cgcgcaggca ggaacggcgt cggaaaaact gctcgcaagg
     8041 cttaaaaata tcggcgtcaa tgactggcaa ccgcatgcta atccggtggt ggtgtgggat
     8101 atctttggcg agaaaggcca tccggtgcgg gcgacggttt cggatctggg gccgctgttg
     8161 ctggcgcggc tgttgaatct caacgatgtg caatctggcg tgctgaatat cattttccgc
     8221 attgctgacg atcaggggct gttactgctc gactttaaag atctgcgggc aattacccag
     8281 tacatcggcg ataacgccaa atccttccag aatcagtacg gaaatatcag tagcgcatcg
     8341 gttggtgcca tccagcgcgg gctgttgtcg ctggaacagc aaggcgcagc acacttcttt
     8401 ggtgagccga tgctggatat caaagactgg atgcgcaccg atgccaacgg taaaggcgtt
     8461 atcaatatcc tcagcgccga gaaactttat cagatgccga aactgtacgc cgccagcctg
     8521 ctgtggatgc tttcagagtt gtatgaacaa ttgccggaag cgggcgatct ggagaagcca
     8581 aaactggtgt ttttcttcga cgaagcacat ctgctgttta acgatgcccc gcaggtactg
     8641 ctggataaga ttgagcaggt gataaggctt attcgctcaa aaggcgtggg cgtctggttc
     8701 gtttcgcaaa acccgtctga tattccggat aatgtgctcg gccagctcgg taatcgcgtt
     8761 cagcacgctt tgcgggcttt tacgcccaaa gatcagaaag cggtaaaagc tgcggcgcaa
     8821 accatgcggg ccaatccggc gtttgatacc gaaaaggcaa tccaggaact ggggaccggc
     8881 gaggcgttaa tctcttttct ggatgcgaaa ggaagtcctt ctgtggtgga gcgtgcgatg
     8941 gtgatcgcgc cttgttcacg gatggggccg gtgacagaag atgagcgtaa tggcttgatt
     9001 aatcactctc cggtgtatgg caagtacgag gatgacgtgg accgggaatc cgcctatgag
     9061 atgttgcaaa aaggcgtaca ggcaagcacc gagcagcaaa ataatcctgc cgtgaaaggg
     9121 aaagaggtgg cggtggatga cggcattctt ggtggattga aggatatttt gtttggcact
     9181 accggaccac gcggcgggaa gaaagatggt gtggtgcaaa caatggccaa aagcgccgct
     9241 cgccaggtga cgaatcagat tgtacgcgga gtgctgggga gtttgcttgg ggggagaaaa
     9301 aggtaagtca ggcgctgttt gtgttctctc acaccaactt gcttttgcct gatgcactac
     9361 gcttatcagg cctacgccac tttgctaaca ataataaagg gagctttagc tccctttatt
     9421 cattcatcaa gttacgattt tctcattaac agccacaggc tgattaagaa gaagctggcg
     9481 cttggcaaca gtgcgccgat gatcggcggg atgccataaa ccaacgtcag cggaccgaag
     9541 atctggtcca gtacgtagaa gacaaaaccg aagctaatac cggtgaccac acgcacaccc
     9601 atcggtacgc tacgcagtgg gccaaagatg aacgacagcg ccatcagcat catcaccgcc
     9661 acggatagcg gctggaagat tttgctccac atgttgagct gataacgtcc ggcatcctga
     9721 ccgctcgact tcagatactt cacatagttg tgcaaaccac tgatggagag tgcatccggg
     9781 tccagcgcca ccacgcccag tttgtccggc gtgaggttgg ttttccaggt gccgctcacc
     9841 gtctgcgaac cggtaatctg tttcggattg gtcagatcag attcatcaac ctgcgacaga
     9901 cgccagactt tatgttccgg gtcaaacttc gcagtagcgg catagcgtac ggattgcaga
     9961 cgacgattct cgttaaaggc ataaatgctg atgccaccta actcttcgtc acctttaacc
    10021 cgctcaatgt agacgaagtt gttgccatct ttcgcccata acccttgctg ggtagagagc
    10081 aacgagccgc cgtacatcgc ctgcgcacgg tagttacgcg ccatctgctc gccctgcggc
    10141 gcgacccatt cgccaatcgc catcgtcagt aagaccagcg gaatggcggt tttcatcacc
    10201 gacagcgcca cctgcatacg ggtaaaacca gaagcctgca tcaccaccag ttcgctgcgc
    10261 tgcgccagca tcccaagacc aagcaacgcc ccaagcagag ccgccatcgg gaagaatatt
    10321 tgcacatctt tcggcacgct cagcaaggta tacattcctg cgcctaacgc gtcgtaactc
    10381 ccctgcccgg cttttttcaa ctgatcgaca aacttgataa tgcccgacag cgacaccagc
    10441 atgaacagcg tcatcatgat ggttgtgaaa atagttttac cgatatagcg gtcaagtacg
    10501 ccaaaaggtt gcatcacacc gctcctttac gcgaaaaact ggcgcgcagg cggcggaccg
    10561 gcacggtgtc ccaaaggttg agaacaatcg ccaaagccag ataaatcagg ttaacggtcc
    10621 acatccacag cgtcgggtcc agcttacctt taccgccgtt cgatttcagg gaggtctgga
    10681 tcaggaagaa aagtagatac agcagcatgg ctggcagcat cgacagtacg cgtccctgac
    10741 gtgggttaac cacgctcagc ggtacgacca taagtgccat cataaacacg gtgaatacca
    10801 acgtgatacg ccagttcagt tctgcgcgag cacgatcggt gtcagtgttc cacaatgtgc
    10861 gcatgtccat ctggtcggta tcgttcgggt cgagcgccac cgcctggtga ccaatgatcg
    10921 cctgataatc ctggaaatcc gtaatgcgga aatcacgtaa cagtgcagtg ccttcgaagc
    10981 gcgttccctg gttgagagtg acgacctggg agccgtcgcg cagctgggtt aaatgtccgg
    11041 aatcggccac caccacagaa ggacgtgcat taccttttgg tcgaatttgt gcgaggaaca
    11101 catctttgaa atcgctgccg tcaacgcttt cgatgaacag caccgagctg ccattagtcg
    11161 cttgctggaa ttgcccttgc gccagcgccg ccatgccagg gttcgctttc gcttctgcta
    11221 acacttcatc ctgatgacgc gatgaccacg gtcccgccca catcacgtta accgccgcga
    11281 cgattgccgt gaataccgca aggatcattg ccgctttcac cagaaccgct ttgctcaggc
    11341 cgcaggcatg cattaccgta atttcacttt cggtatacag tttgcccagc gtcatcagca
    11401 gcccgaggaa caggcttaat ggcaggataa gctgcgccat ttccggcacg cccaacccga
    11461 gaagggagag caccagattc gccggaatat cgccgtcaac cgctgcgccg aggatcctca
    11521 ctaacttttg acagaagaag atcaaaagca agatgaagag tatcgccagc tggcttttga
    11581 gcgtctcccg caccagatat cttatgatta tcactttaaa tacgcccgta aaaactcgtc
    11641 ttttgcagga ttttagcttg tttcatggct taaacgtcat ttattctctt gagtcgtcga
    11701 aatcgtcgct aagataatta tactcaacgg attcacctct cagattttgt tctgacgtgc
    11761 caatgccgta ataacgttaa gattaacacg aagtcatcgc aacagcggac atgagttacg
    11821 aaagcttgca attctatctg tagccaccgc cgttgtcttt aagattcagg agcgtagtgc
    11881 atggagttta gtgtaaaaag cggtagcccg gagaaacagc ggagtgcctg catcgtcgtg
    11941 ggcgtcttcg aaccacgtcg cctttctccg attgcagaac agctcgataa aatcagcgat
    12001 gggtacatca gcgccctgct acgtcggggc gaactggaag gaaaaccggg gcagacattg
    12061 ttgctgcacc atgttccgaa tgtactttcc gagcgaattc tccttattgg ttgcggcaaa
    12121 gaacgtgagc tggatgagcg tcagtacaag caggttattc agaaaaccat taatacgctg
    12181 aatgatactg gctcaatgga agcggtctgc tttctgactg aactgcacgt taaaggccgt
    12241 aacaactact ggaaagtgcg tcaggctgtc gagacggcaa aagagacgct ctacagtttc
    12301 gatcagctga aaacgaacaa gagcgaaccg cgtcgtccgc tgcgtaaaat ggtgttcaac
    12361 gtgccgaccc gccgtgaact gaccagcggt gagcgcgcga tccagcacgg tctggcgatt
    12421 gccgccggga ttaaagcagc aaaagatctc ggcaatatgc cgccgaatat ctgtaacgcc
    12481 gcttacctcg cttcacaagc gcgccagctg gctgacagct acagcaagaa tgtcatcacc
    12541 cgcgttatcg gcgaacagca gatgaaagag ctggggatgc attcctatct ggcggtcggt
    12601 cagggttcgc agaacgaatc gctgatgtcg gtgattgagt acaaaggcaa cgcgtcggaa
    12661 gatgcacgcc caatcgtgct ggtgggtaaa ggtttaacct tcgactccgg cggtatctcg
    12721 atcaagcctt cagaaggcat ggatgagatg aagtacgata tgtgcggtgc ggcagcggtt
    12781 tacggtgtga tgcgtatggt cgcggagcta caactgccga ttaacgttat cggcgtgctg
    12841 gcaggctgcg aaaacatgcc tggcgggcgt gcctatcgtc cgggcgatgt gttaaccacc
    12901 atgtccggtc aaaccgttga agtgctgaat accgacgctg aaggccgcct ggtactgtgc
    12961 gacgtgttaa cttacgttga gcgttttgag ccggaagcgg tgattgacgt ggcgacgctg
    13021 accggtgcct gcgtgatcgc gctgggtcat catattactg gtctgatggc gaaccataat
    13081 ccgctggccc atgaactgat tgccgcgtct gaacaatccg gtgaccgcgc atggcgctta
    13141 ccgctgggtg acgagtatca ggaacaactg gagtccaatt ttgccgatat ggcgaacatt
    13201 ggcggtcgtc ctggtggggc gattaccgca ggttgcttcc tgtcacgctt tacccgtaag
    13261 tacaactggg cgcacctgga tatcgccggt accgcctggc gttctggtaa agcaaaaggc
    13321 gcaaccggtc gtccggtagc gttgctggca cagttcctgt taaaccgcgc tgggtttaac
    13381 ggcgaagagt aattgcgtca ggcaaggctg ttattgccgg atgcggcgtg aacgccttat
    13441 ccgacctaca cagcactgaa ctcgtaggcc tgataagacg cgcaagcgtc gcatcaggca
    13501 atgctgttat tgtcggatgc ggcatgaacg ccttatccga cctacacagc accgaactcg
    13561 taggcctgat aagacgcggc agcgtcgcat caggcaatgc tgttattgtc ggatgcggcg
    13621 tgagcgcctt atccgaccta cacacagcac tgaactcgta ggcctgataa gacacaacag
    13681 cgtcgcatca ggcgctgcgg tgtatacctg atgcgtattt aaatccacca caagaagccc
    13741 catttatgaa aaacgcgacg ttctaccttc tggacaatga caccaccgtc gatggcttaa
    13801 gcgccgttga gcaactggtg tgtgaaattg ccgcagaacg ttggcgcagc ggtaagcgcg
    13861 tgctcatcgc ctgtgaagat gaaaagcagg cttaccggct ggatgaagcc ctgtgggcgc
    13921 gtccggcaga aagctttgtt ccgcataatt tagcgggaga aggaccgcgc ggcggtgcgc
    13981 cggtggagat cgcctggccg caaaaacgca gcagcagccc gcgcgatata ttgattagtc
    14041 tgcgaacaag ctttgcagat tttgccaccg ctttcacaga agtggtagac ttcgttcctt
    14101 atgaagattc tctgaaacaa ctggcgcgcg aacgctataa agcctaccgc gtggctggtt
    14161 tcaacctgaa tacggcaacc tggaaataat ggaaaagaca tataacccac aagatatcga
    14221 acagccgctt tacgagcact gggaaaagca gggctacttt aagcctaatg gcgatgaaag
    14281 ccaggagagt ttctgcatca tgatcccgcc gccgaacgtc accggcagtt tgcatatggg
    14341 tcacgccttc cagcaaacca tcatggatac catgatccgc tatcagcgca tgcagggcaa
    14401 aaacaccctg tggcaggtcg gtactgacca cgccgggatc gctacccaga tggtcgttga
    14461 gcgcaagatt gccgcagaag aaggtaaaac ccgtcacgac tacggccgcg aagctttcat
    14521 cgacaaaatc tgggaatgga aagcggaatc cggcggcacg attacccgtc agatgcgccg
    14581 tctcggcaac tccgtcgact gggagcgtga acgcttcacg atggacgaag gcctgtccaa
    14641 tgcggtgaaa gaagttttcg ttcgtctata taaagaagac ctgatttacc gtggcaaacg
    14701 cctggtaaac tgggacccga aactgcgcac cgctatctct gacctggaag tcgaaaaccg
    14761 cgaatcgaaa ggttcgatgt ggcacatccg ctatccgctg gctgacggtg cgaaaaccgc
    14821 agacggtaaa gattatctgg tggtcgcgac tacccgtccg gaaaccctgc tgggcgatac
    14881 tggcgtagcc gttaacccgg aagatccgcg ttacaaagat ctgattggca aatatgtcat
    14941 tctgccgctg gttaaccgtc gtattccgat cgttggcgac gaacacgccg acatggaaaa
    15001 aggcaccggc tgcgtgaaaa tcactccggc gcacgacttt aacgactatg aagtgggtaa
    15061 acgtcacgcg ctgccgatga tcaacatcct gacctttgac ggcgatatcc gtgaaagcgc
    15121 ccaggtgttc gataccaaag gtaacgaatc tgacgtttat tccagcgaga tcccggcaga
    15181 gttccagaaa ctcgagcgtt ttgctgcacg taaagcggtc gttgctgcgg ttgacgcact
    15241 cggcctgctg gaagaaatta aaccgcacga cctgaccgtt ccttacggcg accgtggtgg
    15301 cgtagttatc gaaccaatgc tgaccgacca gtggtacgtg cgtgccgatg tactggcgaa
    15361 accggcggtt gaagcggttg agaacggcga cattcagttc gtaccgaagc agtacgaaaa
    15421 catgtacttc tcctggatgc gcgatattca ggactggtgt atctctcgtc agctgtggtg
    15481 gggtcaccgt atcccggcat ggtacgacga agcaggtaac gtttatgttg gccgcaacga
    15541 agaagaagtg cgcaaggaaa ataacctcgg tgccgacgtt gccctgcgtc aggacgaaga
    15601 cgttctcgac acctggttct cttctgcgct gtggaccttc tctaccctag gctggccgga
    15661 aaacaccgac gcactgcgcc agttccaccc aaccagcgtg atggtatctg gtttcgacat
    15721 catcttcttc tggattgccc gcatgatcat gatgaccatg cacttcatca aagatgaaaa
    15781 tggcaaaccg caggtgccgt tccacaccgt ttacatgacc ggcctgattc gtgatgacga
    15841 aggccagaag atgtccaaat ccaagggtaa cgttatcgac ccactggata tggttgacgg
    15901 tatttcgctg ccagaactgc tggaaaaacg taccggcaat atgatgcagc cgcagctggc
    15961 ggacaaaatc cgtaagcgca ccgagaagca gttcccgaac ggtattgagc cgcacggtac
    16021 tgacgcgctg cgcttcaccc tggcggcact ggcttctacc ggtcgtgaca tcaactggga
    16081 tatgaagcgc ctggaaggtt accgtaactt ctgtaacaag ctgtggaacg ccagccgctt
    16141 tgtgctgatg aacacggaag gtcaggattg cggcttcaac ggtggtgaaa tgacgctgtc
    16201 gctggcggac cgctggattc tggcagagtt caaccagacc atcaaagcgt accgcgaagc
    16261 gctggacagc ttccgcttcg atatcgctgc aggcattctg tatgagttca cctggaacca
    16321 gttctgtgac tggtatctcg agctgaccaa gccggtaatg aacggtggca ccgaagcaga
    16381 actgcgcggt actcgccata cgctggtgac tgtactggaa ggtctgctgc gcctcgcgca
    16441 tccgatcatt ccgttcatca ccgaaaccat ctggcagcgt gtgaaagtac tttgcggtat
    16501 cactgccgac accatcatgc tgcagccgtt cccgcagtac gatgcatctc aggttgatga
    16561 agccgcactg gccgacaccg aatggctgaa acaggcgatc gttgcggtac gtaacatccg
    16621 tgcagaaatg aacatcgcgc cgggcaaacc gctggagctg ctgctgcgtg gttgcagcgc
    16681 ggatgcagaa cgtcgcgtaa atgaaaaccg tggcttcctg caaacgctgg cgcgtctgga
    16741 aagcatcacc gtgctgcctg ccgatgacaa aggtccggtt tccgttacga agatcatcga
    16801 cggcgcagag ctgctgatcc cgatggctgg cctcatcaac aaagaagatg agctggcgcg
    16861 tctggcgaaa gaagtggcga agatcgaagg cgaaatcagc cgtatcgaga acaagctggc
    16921 gaatgaaggc tttgtcgccc gcgcaccgga agcggtcatc gcgaaagagc gtgagaagct
    16981 ggaaggctat gcagaagcga aagcgaaact gattgaacag caggctgtta tcgccgcgct
    17041 gtaatcaaaa cacagtgatg aaaacgaagg ccggagcatg ctccggcctt ttttatctct
    17101 tacagaaatg gtaaagaagg cataattccc cgtgaaatac tggctaagaa gcctttatct
    17161 ggttgttctt ctttattgat taaaggaagg tcatccaaat cgcccagcaa atacgtattt
    17221 tttgcctgcc agtcaatcat ccatattttc agcagtggat aagccagacc gcccgtaatc
    17281 ccggatatca ctaccaacgc acacatgcga taaagcatac cgtggtatgt taaagttgag
    17341 cgaaaacgaa tacgcccatc attcagtgac aggttgctca taaaatgatt tcgcaacgac
    17401 accgttaaat aactggtgct aacagcaatc ccaaaataat agattaactg cgcgattatc
    17461 attttacgtt gcatttccat aaattgctgt aaattctcaa tatcatcatt tgcatacaca
    17521 gaactatcat acgcatttaa tatttgatcg aagataatat aaccagcaac agcgagaaaa
    17581 ggtaataaag ctaaaaatgc gaggatggca tattttatac aatacgcagt atctaatttc
    17641 accttgaaac gatgtatacc gaaactggta ttgctccaga gaaaactcat taccagacta
    17701 tataaagtac cattaaaaac accaatggaa acaataccaa ctattgccat cagaaccaca
    17761 gatataataa cactatttga actattggca tgtagcatct ttgtcgagat aaagaaaaca
    17821 gtccccatcc caatggccat taaaatcggc aagaaaaagg ttacccacca gaaccctttc
    17881 aaagagcaat taaaactaaa tcttacgccg ttgagactgg tcatcaaggc ctgataacgc
    17941 agtccttttg ctgccataaa tataagcaaa accaacagtg acaaagtcaa aacacagcca
    18001 acaagcggca tatctgctga cactgtcatt aagattgcga aatagaaaaa aacaaaaaca
    18061 agacaactaa caaaaacatt cccaccagta attccataag aaaatcgttg tccgttaact
    18121 tccatattag catagagata acgcttacat ttcattaatg cccatggtaa atagatacct
    18181 agcgttataa tcgttaacaa cacattcacc acacaaataa gaaaatatct ctcacctgta
    18241 ccatgaaaaa caaacgaatg ggacggaaca tccatttcat taataacttg agccataaaa
    18301 attccctgaa ttaaaaaatc cat
//
LOCUS       DAZFFG010000091        18263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFFG010000091 DAZFFG010000000
VERSION     DAZFFG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.091"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..2245
                     /locus_tag="ACHJ7J_003562"
     CDS             <1..2245
                     /locus_tag="ACHJ7J_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO5001746.1"
                     /translation="LFFMTGYACATEWISSEDLIISDFHLMTADERNVVKAATDDSME
                     AAYMLKDNIRWYYHNGNLSLPANFSNQNKLVVNGNLTISGDYDDYLSGNGHLIVLGNV
                     IVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVSDKATQFEVIKAE
                     FYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPDYDSVADNIVQGL
                     PLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTESLSPAVMLQLLQ
                     HPDDQTRESMAQSWPAQQMHLLTDELIKDEAVARGLVKNSNISADVNKKLMSVPVESV
                     QLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVADELINNEDPELRE
                     RITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTEDIERIAEQMYLD
                     NKENKNIVKALLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISYLLTQHDVPTVWD
                     ELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNGVVDEEMLNNAID
                     LLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVVSMKNSTRRQSER
                     GLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASLLTTLT
                     EPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRKIRSEAR
                     HRLEEKQ"
     gene            2242..2697
                     /locus_tag="ACHJ7J_003563"
     CDS             2242..2697
                     /locus_tag="ACHJ7J_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001747.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(2775..3686)
                     /gene="rdgC"
                     /locus_tag="ACHJ7J_003564"
     CDS             complement(2775..3686)
                     /gene="rdgC"
                     /locus_tag="ACHJ7J_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO5001748.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3811..4719
                     /gene="mak"
                     /locus_tag="ACHJ7J_003565"
     CDS             3811..4719
                     /gene="mak"
                     /locus_tag="ACHJ7J_003565"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO5001749.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4862..6046)
                     /gene="araJ"
                     /locus_tag="ACHJ7J_003566"
     CDS             complement(4862..6046)
                     /gene="araJ"
                     /locus_tag="ACHJ7J_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO5001750.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(6172..9315)
                     /gene="sbcC"
                     /locus_tag="ACHJ7J_003567"
     CDS             complement(6172..9315)
                     /gene="sbcC"
                     /locus_tag="ACHJ7J_003567"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO5001751.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGQAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLIASLQVLTDEEKQLLTAQQQEQQSLNWLTHLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLNAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQREQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRIQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(9312..10514)
                     /gene="sbcD"
                     /locus_tag="ACHJ7J_003568"
     CDS             complement(9312..10514)
                     /gene="sbcD"
                     /locus_tag="ACHJ7J_003568"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO5001752.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQTLTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            10704..11393
                     /gene="phoB"
                     /locus_tag="ACHJ7J_003569"
     CDS             10704..11393
                     /gene="phoB"
                     /locus_tag="ACHJ7J_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO5001753.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            11451..12746
                     /gene="phoR"
                     /locus_tag="ACHJ7J_003570"
     CDS             11451..12746
                     /gene="phoR"
                     /locus_tag="ACHJ7J_003570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO5001754.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            13153..14472
                     /gene="brnQ"
                     /locus_tag="ACHJ7J_003571"
     CDS             13153..14472
                     /gene="brnQ"
                     /locus_tag="ACHJ7J_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO5001755.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            14548..15921
                     /gene="proY"
                     /locus_tag="ACHJ7J_003572"
     CDS             14548..15921
                     /gene="proY"
                     /locus_tag="ACHJ7J_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO5001756.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            16077..17894
                     /gene="malZ"
                     /locus_tag="ACHJ7J_003573"
     CDS             16077..17894
                     /gene="malZ"
                     /locus_tag="ACHJ7J_003573"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO5001757.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PVRLEQFAIDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIVLRKKSQALCRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTEGILALPAISATVWMN"
     gene            18079..>18263
                     /locus_tag="ACHJ7J_003574"
     CDS             18079..>18263
                     /locus_tag="ACHJ7J_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001758.1"
                     /translation="MTISNSNLLKPFITGSKLLPFKDDVFSKTEEQFKKSPHGKSLFI
                     SGNENLVNISAEKIDEI"
BASE COUNT         4259 a         4488 c         4846 g         4670 t
ORIGIN      
        1 cttatttttt atgactggct atgcatgtgc cacagagtgg atcagtagcg aggaccttat
       61 catctctgac tttcatctta tgacggcaga tgagcgcaac gtggttaaag cggcaaccga
      121 cgatagtatg gaagctgctt atatgctaaa agataatatc cgctggtatt atcataatgg
      181 taacctatca ttacccgcta acttcagcaa tcaaaataaa ctggtggtga acggtaattt
      241 aacgatttcc ggcgattatg atgattatct gtcaggtaat ggtcatctga ttgtattagg
      301 taatgttatt gtcgataact ttattaatca tgatttcgcg tatgtcaaag gacagatgac
      361 agcgaaaggt ttagtttatg ctgattacaa tgatcataac tttgaagtga tgaagggaat
      421 tagtgcgcga ggcattattg tttccgataa agcaacgcaa tttgaagtca ttaaagctga
      481 attttatatc aatgaagatg gaagtggtga aggatataac tgggatgaga atattcaaaa
      541 agcctattca ctggtgactg ccgatcttta tgatcacact gaaatcgaaa ccgataacat
      601 ttccaatgcg tatcctgact atgatagcgt cgcagacaac atcgttcagg gtttaccgct
      661 ttttcgagat aaagccgctc ctgaaattaa tgagaaatta aaatggattg aaacggggaa
      721 actcgataat ttcccagcga ataaaataaa gcatcaagat ccactggtgg cacgttttct
      781 gactcataca gagagtttat cacccgccgt tatgctgcaa ctgttacagc accccgatga
      841 ccagacgcgt gaatcgatgg cgcagtcctg gcccgctcaa cagatgcatt tgcttaccga
      901 tgaattaatt aaagatgagg ctgttgccag agggctggtt aaaaacagta atatttccgc
      961 cgatgtgaat aaaaaattaa tgtctgtacc cgtcgagagc gtacagctgg agcaggccag
     1021 acaggataat ctttctccag atattgttgc gtcgttaagt catagcccct ttctcagtgt
     1081 acgcaagaca ttactcagcc attacgatta cgcctggctg gttccgactg cagtggccga
     1141 tgagttgata aataacgaag acccggagtt acgtgaaaga attaccggag cagatttaac
     1201 cgcacaacaa gcagtgatgt tgagtaaaga taaatcactg aaagttcgtg aagcattagc
     1261 ccggacgctt actgaattaa aaattaccca actgtcagca accctgcgta cagaggatat
     1321 tgaacgtatc gcagaacaaa tgtatctgga taataaagaa aataaaaata tcgtaaaggc
     1381 gttattgata gcgttgccgg agatgcgtca actgtctctg gcaaaagaag atgtgcacaa
     1441 tttgcgcgaa ggtgcgcgtt atctgacatc gaaggacgtg attagttatc tattgactca
     1501 gcatgatgtt cccaccgtct gggatgagct ggcacgcgat aaattattgc cgctggaata
     1561 taagaaacag ttatggcaac gcacattaaa tttaatgatg agcaaacggc aagaagatca
     1621 agagcaagcc tacgaagtac agctggcgct gattgataat ggtgttgttg acgaagagat
     1681 gttaaataat gccattgatt tactggttga tttacccgct gaataccgct atcgaatgcg
     1741 taaccaatta tttgataata aagaattacc ttccggaatt atcaacaaac tcgatcaaca
     1801 gtatcgcttc aactctgact gggcgctggc tgttgtcagt atgaaaaata gcacccgtag
     1861 acaaagtgaa agaggattac accgctggaa ccatgaggat agcgatattt ttgcggaact
     1921 ggcgacgata aaagataaat cagatgatga atggtggcgt gcattgctgc aaagtcgcaa
     1981 tgatcatttg cgccaaacgg cattacgcaa cgcacatacc ccggcgtcgt tgttaacgac
     2041 attgactgag cctcaggatc ggtcgctggc tattaataat ccacagctgg ctgccgatgt
     2101 gaaaacggcg tggttaaaag aggatccatc attactctta tttgtcgaac aacccgatct
     2161 ttcgcagtta cgggatttag tgaaaaccgg ggcaacgcgg aaaattcgca gtgaagcgcg
     2221 tcaccggctt gaggaaaaac aatgacacag cgcccatgga gcaagttaca gcgggagatc
     2281 tatgacttac tgacgccaac gatcaacctg caaattcact gtacccgtta tcccatgcgc
     2341 agccaaaatg gtggaagcac cgatttaccg cgttattgga tcacgttgga taaaaacgtg
     2401 atctgggatt acccgaaaga ctttattgca ggtaacggtg gagtgcgtaa ttttcacggt
     2461 gaaacttgct ggtatcctta cctgacggat atttgttcaa tttctgattt attgcgtgaa
     2521 tatattgaca ccccaaaagc ggagctgtta acaaaacaat ttaccagcga taaatggggg
     2581 ttagtgaata ttctgcgtgc tgctgacagg cgaataggga tgaggcgatt agatcagtta
     2641 cggcgtaaaa cgcataatat tgctgcgcta aaaattattg cccgccgtag cgaataatta
     2701 tatgcctggt gtggcttcgt acgccggata agacgcggca ggcgtcgcat ccggcattaa
     2761 aggaaaatca gcaattaacg ttgtgcttcg ccacctaatc cttcaatcag gttttgaatt
     2821 aacgctgcca gttcaccagt catcaggatg aaatcggcat caaaacgctg ggcgaaatct
     2881 tcacggtcga tatcttcgtt ttgatcgcgc agctcgtcgc agaacttcag acgcttgagc
     2941 gaaccatcgt cgcacatcac aaactgaatg cgctgctgcc agtcgagcgc cagtttagtc
     3001 accacttttc cggcttcaat gtgattggtg atctcttcgc tggtcagatc ttgtttcttc
     3061 gcgcggatca ctccgccatc ttccagcaac gatttcagct cggcttcatc aagcagctgg
     3121 aagccctgtg ccgcactacc ggaacgtacc cattcggtca gcgtcagttc aatcgggttt
     3181 tccatgctca atggtacaac cggtaacgac cccaggcttt tacgcagcag tgccagcgta
     3241 tcttccgctt ttttggcact ggcgcagtcc accataatca aaccgttaac cgtgtcgatc
     3301 cacatcattg tctggctaaa acggctgaaa gcacgcggca acagagagtg cagcacttcg
     3361 tctttcagcg aatctttttc ggttttcttc agcttacgcg cctgttccgc ttccagtttg
     3421 gcgattttcg cttccagcgc ctgtttaatc accggagacg ggaggatttt ttcttctttg
     3481 cgcgcgcaga taacaatttg accattggca acgtgcgtta acgcatcgct gtgcgatccc
     3541 atcggaggaa cccagcccat cttcgccatg tcctggctgc cgcatggggt aaatgccatc
     3601 gaggctagct gtttttccat ctcttctgca cgcagcgaaa tctcgcggct aagacggtaa
     3661 accattaaat ttttgaacca cagcatgata atttccacgg ccttgtcgtt aaatttagcg
     3721 ggcatgataa cgaattgtcg gcggccttgc attgccaatc cggttgtccg tctctacgct
     3781 attgatattg aaaaaaataa ggagagtacc gtgcgtatag gtatcgattt aggcggcacc
     3841 aaaactgaag tgattgcact gggcgatgca ggggagcagt tgtaccgcca tcgtctgccc
     3901 acgccgcgtg atgattaccg gcagactatt gaaacgatcg ccacgttggt tgatatggcg
     3961 gagcaggcga cagggcagcg cggaacggta ggtatgggca ttcctggctc aatttcgcct
     4021 tacaccggtg tggtgaagaa tgccaattca acctggctca acggtcagcc attcgataaa
     4081 gacttaagcg cgcggttgca gcgggaagtg cggctggcaa atgacgccaa ctgtctggcg
     4141 gtttcagaag cagtagatgg cgcggcagcg ggagcgcaga cggtatttgc cgtgattatc
     4201 ggcacgggat gcggcgcggg cgtggcattc aatgggcggg cgcatatcgg cggcaatggc
     4261 acggcaggtg agtggggaca caatccgcta ccgtggatgg acgaagacga actgcgttat
     4321 cgcgaggaag tcccttgtta ttgcggtaaa caaggttgta ttgaaacctt tatttcgggc
     4381 acgggattcg cgacggatta tcgtcgtttg agcggacatg cgctgaaagg cagtgaaatt
     4441 atccgcctgg ttgaagaaag cgatccggta gcggaactgg cattgcgtcg ctacgagctg
     4501 cggctggcaa aatcgctggc acatgtcgtg aatattctcg atccggatgt gattgtcctg
     4561 gggggcggga tgagcaatgt agaccgttta tatcaaacgg ttccgcagtt gattaaacaa
     4621 tttgtcttcg gcggcgaatg tgaaacgccg gtgcgtaagg cgaagcacgg tgattccagc
     4681 ggcgtacgcg gcgctgcgtg gttatggcca caagagtaaa aaacccaggc aattggcgca
     4741 tcatgcctga tgcgacgctt gccgcgtctt atcaggccta caaaatgtgc cagaaccgcg
     4801 tagggcggat aaggcgttca cgccgcatcc ggcaataagc aacgcgcacc gtgacagtaa
     4861 tctaccccag tggtttcgcc agcaccggac tatccgccgc ttgctggcgc ttatagcgac
     4921 catacagcag caacgatgac atcgcagcaa acgaaagcag ggcggcaggc agcgccacgt
     4981 aattatatgc cagccccagc gtcagcatca tacctccgca atatgcgccg acggcgctac
     5041 cgaggttaaa cgctatttgc ccacctgcgg cacctaataa ctctccgcct ttggcatttt
     5101 gcagtaacaa tatttgcaga ggtgctgaaa gggcaaataa tcccgcgcaa caaataaaag
     5161 caaaaataag cgacgttgtt ttcatgccgc cgcagaaaaa gagcatcagc agtgccagta
     5221 caattataaa gtcagtcact gctgcaatgc gcagtggtga ataacgtcct gaaatcctgc
     5281 cacttagcat atttcccagc accatcccta gcccaactaa catcataata aaggtcatcg
     5341 ccgtttccga aaaaccggaa ataaacatca tgtatggctt tacgtagctg aaccaggcaa
     5401 acacacctgc gttgccaaac atggtggcgg cgaaaattaa ccacggggcc gggctgcgca
     5461 aaaagtgaaa ttgttcgcgc agctttcctt tcgcctcgtc gcgaatatcc ggcacccaaa
     5521 aatagaccga tgccatcacc gcaatattaa aaacagcgat caataaaaag gtgtaacgcc
     5581 agctaaattc ctgacttaaa tacgttccca gcggaatgcc cagcaaattg gcgactgtca
     5641 tcccggaaac catccccgcc acggcggcgg tgacttttcc gggtttgata atttttgata
     5701 acacgatcgc tccgacgcca aaaaatgcgc catgcggaaa gccggaaacc aggcgaccaa
     5761 tggcaagcat caggtaagac gaagagagcg tgaacatggc gttgccaatg acgcacaacg
     5821 ccaccagaaa caacaagata tgtttgagtg agtagcggct ggaaaagagt gcgattattg
     5881 gcgcaccgac caccaccccc agcgcataat acgagatcat atgcccggca gcaggaatcg
     5941 aaattcctac gttatgcgcc agctccgtga gcacgcccat aatgccaaat tcggccatcc
     6001 ccaaaccaaa cgtgcccaga gccaaagata aaatgacttt tttcatacca ccatcacaac
     6061 caccagaacg gctaccaatc accgtagcca ataaagtgat agcttgcagg ataacgacca
     6121 gcaaggccaa gagataattc gcccctctgt attcattatc ctgctgaata gttatttcac
     6181 tgcaaacgta ctttccagtt tgctgtagcc cagtccattg atctttttca ctttgatctg
     6241 caccggaata cgctctttca tcgcttctac gtggctaatc acaccgatgg ttttgccact
     6301 ggcgttcagg gcatccagcg catcaagggc ggtatccagc gtttcgctat ccagcgtgcc
     6361 aaaaccttca tcaaggaaca gcgagtcaat acgcgtttta tggctgacca gatctgaaag
     6421 cgccagcgcc agcgccagac tgacgaggaa actttcgccg ccagaaaggg tacgggtgtc
     6481 gcgtaccgca tctgcctgcc aggtatcaac aacctcgact tccagcgcct cgctggcttt
     6541 gcgctgtaac agatagcgcc cgtgcagccg ggtaagttgc tgattagcga gatggactaa
     6601 attatccagc gtcagcccct gggcaaactt gcggaattta tcgccctctt tggaacctat
     6661 tagcgaattc agatatcccc agtcctcaac ctgctgcgtc atttgagcaa tttgctgcag
     6721 taaggtttgt tgttgctgac ggttatctgc atcctgcttc agctgctggc gaatctcgcc
     6781 ttgactcgtg gtgttctcac gcaatttttg gtgagtttgc gctaactctt gctgaatctg
     6841 ctccaccgtc acagtgagag ccaacccgtc aggtcggtgt tgctgatgct gtgccagcgt
     6901 ttctgctgtc tgagtgacca gcgtttgcgc ctgacggcgc tggttttcca gattctgctt
     6961 gagctgttcc agttgcgtta gtgtttgttc atccattagc gccgcaagga acgcctgctg
     7021 atcgtcaaag acgctggcct gtagcgcggt gtcaaactgc gcctgggctt tttgcagact
     7081 ttgcgccgcc agaacgtcct gttgctgtaa cgtctgctgc tggctgtgta atgcgagaca
     7141 ttgctcatgt acctgccgcc agttttccag taccacagtt tcttcgctgt gcgggagttc
     7201 atcactttgc ggcaacgttt ccagaatcgg cgtcagctgc tgaatacggt tttgcagcgc
     7261 ggttaattcg ttctggcgat gctgccagct ctgcgcttct tgctgacgtg tcgccaacca
     7321 gctctcttct tcatcttcct gtggcaatgt cagtgcataa cccgtcaatg ccgttaagag
     7381 ttgctgctcg cgttgttcaa tttgctgttg atactggata atttgctgat tatgcgcggc
     7441 aatctgccct tgtaattcat gccgttggct gagtaaccgc agctggcgtt cgtgctcatc
     7501 ttgtgcattc agccacggtt gaatatcgtc ctgtggctgc aaggtgatat tgaggctggt
     7561 cgtgacggct tgccattgtt gagtaagtgc ttgctcatct tgtcggaggc tttgcgcttc
     7621 gttttcatca cgctgaagct gctttgttaa ggcgtccagt tgcccacgta gcgtcgcacc
     7681 ttcttcaccg agctttttaa cttcgttttc cagcgccagt aatcgagact gattaacacc
     7741 aggctccagc gcctgatacg cctcgaccgc cgggtggctg gtggaaccac aaagtggaca
     7801 aggctgaccc gcctgtaact gtgcacgttg agcttccagc gttttgatgc gcgcttcctg
     7861 ctcgcaaatg gttttgacat cggcaagttg ctgcgtcttt tctttataac gctggcgcat
     7921 ttcgttaagt gcggcattac gttgcgtctg ttccagagtg acattctgga tagtgaccat
     7981 taactgcgcc agacgttttt gttggggaac aatctgccca tgcagcgcga ccaggcgctg
     8041 acgcagtggg cgttgctcgg catgttgcgc cagggcgcta gcaacttcat cggcggttaa
     8101 cgtcaacgtg atcgccgcaa gcgtattaag tttttgctca gcatgggtta actgttgctg
     8161 ccattgccgc agatgctcgc gatcgctggt ttgttgggag aactgcgcac gccaacccgc
     8221 cagttcgttg ttccactgac ggaagcggtc gtgttcctgt aaccaggtat tcaggctttg
     8281 ttgctgctgc tgtaattctg ctgactgctt cgccgcgtgg tggcgaatgc tcgcgcgaag
     8341 cgccattgtg ctctgtaagc gagtatttac ttcttcaatc tgctggcgag tatgcgccag
     8401 cgccgcgctg tgttctgcga tgcgttccca gtgcggacga agatttcgtg ccggttgtgc
     8461 cagactgagc gccgccagtt gaggttgcgc tttttcttct tcggctaacg cctgttgcaa
     8521 ggcctgctga cggcggctgg cttcttgctg caattcgtcc agatgcgtta accagtttag
     8581 tgattgttgt tcttgctgct gcgcggtaag taactgtttt tcttcgtcag taagtacctg
     8641 caaactcgct atcagcgatt gcacttgttc cggcgtgagc aacgcgacgc cgctggcctg
     8701 cgcttgcagc ttctccagct cagtgcgggc cgatttgtgc tgctcaaaaa ccatcgccga
     8761 gatttgcccg tagatttcag tgccggttaa ctcctcgagc aattccgcgc gttcttttgg
     8821 tttggcattc aggaaggcag caaattgccc ctgcgaaagc agcatcgaac gggtgaagcg
     8881 cccgtaatcc agtccggtta acgtcgccgt cagttccagc ttatctttca ctttgtcggc
     8941 gagaattttg ccatcggcgc agcgcgccag ctctacgcgt ggcacctgca aattaccgtc
     9001 gggttggtta cgcgcccgat tctggctcca gaacgcgcga tacgcttgac ctttcacttc
     9061 aaactccacc tccgccagac attcggcggt atcacgggtc atgagatcat tttgcgattg
     9121 tgaaacgtta gagaggcgcg gagtttcgtg atacagcgcc agacaaatgg cgtccagcag
     9181 ggtggttttc cccgcaccgg ttgggccggt aatggcaaac agcccgttgc tggcgaacgg
     9241 ctcgcgggtg aaatcaatct tccattcgcc ttttaatgag ttcaggtttt tcaggcgcag
     9301 gctgagaatt ttcatgcttc gtgttctccg gcgaggctat gcaacgtcgt ggcgaaaaga
     9361 tgctgcagac gttgctgctg cgattcatcc agttcttcca gtgccagacg gcgattgaac
     9421 acctcttcga cgctgagttc gctgagggtt tcacgctgtt ggctggctaa cacacgctcg
     9481 cgctgttcac gactccggcg taccagcaat acctcaacag gcaatgattc ggttaatgtc
     9541 tggattttgc gctgaatatc atgcagatac tcatcagtag tgatttcgat atccagccag
     9601 acaggtggct cctgcgatac atcgcgccac tgttccagct gttcggtaat cgacgccaga
     9661 tcgcctttca gcaccgccat gggttgcgtt accggtacgt tcaggttttc cacgctctct
     9721 aatttgccgt ttgaaaatgt caccagatgg acatatttac tctttccgca ttcatcaaaa
     9781 ctcagtggaa tgggggagcc gcaatagcga acatgttcca tgccgccaat aatctgtgcg
     9841 cggtgaatat gcccgagcgc gatgtagtcg gctggtggaa agttttgtgc cggaaacgcg
     9901 tccagcgtgc caatataaat gtcacgcacg gcgtcacttt tactggcccc cacggtcgtt
     9961 aaatgtcccg tggcgatgat gggcagaggc tgatcgccgc gcagtttgca ggcatcggca
    10021 tagtgttgtt ggtaataatc ggtaatcgct gccagtaaat gctgctgttt ttcaataccg
    10081 ttaagccccg cctggctggt aataatgtca cgcggacgta aaaacggaat ggggcacagc
    10141 actgcgcctg gcgtcccgtc gcgacgaggc aagatttgcg gcgcatgtcc ggcgctggcg
    10201 accacggtag tattgaggaa cgccatgata tcgcgcgatt cattcagcgt ggcgaccgag
    10261 tcatggtttc ctgccagtac caccagatga cagccagttt gctgtaaatt gacaacaaaa
    10321 cggttgtata acgtgcgggc gtaactgggc ggcgagccgg tatcgaaaac atcaccggca
    10381 acaataatcg catccacctg atgggtttgt gctgtttcca gcagccagtc aagaaaagcc
    10441 tgatgttcag cttcgcggct tttactatag aagttctggc cgagatgcca gtctgaggtg
    10501 tgaaggatgc gcataacggt tccctggcga aaaagcatgg gcgcgattat acccaaacag
    10561 atgtgccatt tgcttttttc tgcgccacgg aaatcaataa cctgaagata tgtgcgacga
    10621 gcttttcata aatctgtcat aaatctgacg cataatgacg tcgcattaat gatcgcaacc
    10681 tatttattac aacagggcaa atcatggcga gacgtattct ggtcgtagaa gatgaagctc
    10741 caattcgcga aatggtctgc ttcgtgctcg aacaaaatgg ctttcagccg gtcgaagcgg
    10801 aagattatga cagtgctgtg aatcaactga atgaaccctg gccggattta attctccttg
    10861 actggatgtt acctggcggc tccggtatcc agttcatcaa acacctcaag cgcgagtcga
    10921 tgacccggga tattccagtg gtgatgttga ccgccagagg ggaagaagaa gatcgcgtgc
    10981 gcggccttga aaccggcgcg gatgactata tcaccaagcc gttttcgccg aaggagctgg
    11041 tggcgcgaat caaagcggta atgcgccgta tttcgccaat ggcggtggaa gaggtgattg
    11101 agatgcaggg attaagtctc gacccgacat ctcaccgggt gatggcgggc gaagagccgc
    11161 tggagatggg gccgacagaa tttaaactgc tgcacttttt tatgacgcat cctgagcgcg
    11221 tgtatagccg cgagcagctg ttaaaccacg tctggggaac taacgtgtat gtggaagacc
    11281 gcacggtcga tgtccacatt cgtcgcctgc gtaaagcact ggagcccggc gggcatgacc
    11341 gcatggtgca gaccgtgcgc ggtacaggat atcgtttttc aacccgcttt taacgccttg
    11401 ctcatcggac gcagagcagg gcttatgatt tcttaactgg agtatcttac gtgctggaac
    11461 ggctgtcgtg gaaaaggctg gtgctggagc tgctactttg ctgcctcccg gctttcatcc
    11521 tgggtgcatt ttttggttac ctgccctggt ttttgctggc atcggtaacg ggactgctta
    11581 tctggcattt ctggaattta ttgcgccttt catggtggct gtgggtggat cgtagtatga
    11641 ccccgccacc ggggcgtggt agctgggaac cgctactata cggcttacac cagatgcagc
    11701 tgcgaaataa aaaacgccgc cgtgaactgg gcaatctgat taaacgcttt cgtagcggcg
    11761 cggagtcgct gcccgacgca gtggtgctga ccacggaaga gggcggtatt ttctggtgta
    11821 acggtctggc gcaacagatt cttggtttgc gctggccgga agataacggg cagaacatcc
    11881 ttaacctgct gcgttacccg gagtttacgc aatatctgaa aacgcgtgat ttttctcgcc
    11941 cgctcaatct ggtgctcaac accgggcggc atctggaaat tcgcgtcatg ccttataccc
    12001 acaaacagtt gctgatggtg gcgcgtgatg tcacgcaaat gcatcaactg gaaggggcgc
    12061 ggcgcaactt tttcgccaac gtaagccatg agttacgcac gccattgacc gtgttacagg
    12121 gttacctgga gatgatggat gagcagccgc tggaaggcgc ggtacgcgaa aaagcgttgc
    12181 acaccatgcg cgagcagacc cagcggatgg aagggctggt gaagcaattg ctgacgctgt
    12241 cgaaaataga agccgcaccg acgcatttgc tcaatgaaaa ggttgatgtg ccgatgatgc
    12301 tgcgcgttgt tgagcgtgag gctcagactc tgagtcagaa aaaacagaca tttacctttg
    12361 agatagataa cggcctcaaa gtgtctggca acgaagatca gctacgcagt gcgatttcga
    12421 acctggtcta taacgccgtg aatcatactc cagaaggcac gcatatcacc gtacgctggc
    12481 agcgagtgcc gcacggtgcc gaatttagcg ttgaagataa cggaccgggc attgcaccgg
    12541 agcatattcc gcgcctgacc gagcgttttt atcgcgttga taaagcgcgt tcccggcaaa
    12601 ccggcggtag cggattaggg ttagcgatcg tgaaacatgc ggtgaatcat cacgaaagtc
    12661 gcctgaatat tgagagtaca gtaggaaaag gaacacgttt cagctttgtt atcccggaac
    12721 gtttaattgc caaaaacagc gattaatccg cctttgtcat cttttattgc cataagccag
    12781 ccaatgctgg cttattttct ttgcagtcaa aatacgggcg ttagatttta caacggttgg
    12841 tgattttttg ttcgcatgat tagccatgtc tttttcacgg aaatagtgtt ttatactggt
    12901 tggtgatttc ttatcgctat atacctctgg tttttagatc cctccttgct ttaaaacgtt
    12961 ataagcgttt aaattgcgct tcaggtgctg tcatactgac tgcattaacg cggtaaatcg
    13021 aaaaactatt cttcgccgcg cctggttggg agtatttccc gctaaaattg tttaaatata
    13081 ccgctgtatc atccccaggg attggcacaa aaatttaacg ttacaacacc acatccacag
    13141 gcagtatgat ttatgaccca tcaattaaga tcgcgcgata tcatcgctct gggctttatg
    13201 acatttgcgt tgttcgtcgg cgcaggtaac attatttttc ctccaatggt cggcttacag
    13261 gcaggcgaac acgtctggac tgcggcattc ggcttcctta ttactgccgt tggtctgccg
    13321 gtattaacgg tagtggcgct ggcaaaagtt ggcggcggtg ttgacagcct cagcacgcca
    13381 atcggtaaag tcgctggcgt actgctggca acggtttgtt acctggcggt ggggccgctt
    13441 ttcgctacgc cgcgtacagc taccgtttcc tttgaagtgg ggattgcgcc gctgacgggt
    13501 gattccgcgc tgccgctgtt tatctacagc ctggtctatt tcgctatcgt tattctggtt
    13561 tcgctctatc cgggcaagct gctggatacc gtgggcaact tccttgcgcc gctgaaaatt
    13621 atcgcgctgg tcatcctgtc tgttgccgcg attgtctggc cggcgggttc tatcagcacg
    13681 gcgactgagg cttatcaaaa cgctgcgttt tctaacggct tcgttaacgg ctatctgacc
    13741 atggatacgc tgggcgcaat ggtgtttggt atcgttattg ttaacgcggc gcgttctcgt
    13801 ggcgttaccg aagcgcgtct gctgacccgt tataccgtct gggctggcct gatggcgggt
    13861 gttggtctga ctctgctgta ccttgcgctg ttccgtctgg gttcagacag cgcgtcgctg
    13921 gtcgatcagt ctgcaaacgg cgctgctatt ctgcatgctt acgttcagca cacctttggc
    13981 ggcggcggta gcttcctgct ggcggcgtta atcttcatcg cctgcctggt aacggcagtt
    14041 ggcctgacct gtgcttgtgc agaattcttt gcccagtacg taccgctctc ttatcgtacg
    14101 ctggtgttta tcctcggcgg cttctcgatg gtggtttcta acctcggctt aagccagctg
    14161 atccagatct ccgtaccggt gctgaccgct atttatccgc cgtgtatcgc actggttgta
    14221 ttaagtttta cacgctcatg gtggcataat tcgtcccgcg tgattgctcc gccgatgttt
    14281 atcagcctgc tttttggtat tctcgacggg atcaaagcat ctgcattcag cgatatctta
    14341 ccgtcctggg cgcagcgttt accgctggcc gaacaaggtc tggcgtggtt aatgccaaca
    14401 gtggtgatgg tggttctggc cattatctgg gatcgcgcgg caggtcgtca ggtgacctcc
    14461 agcgctcact aaatcactga acatttgttt taaccacggg gctgcgatgc cccgtggttt
    14521 tttattgtgt tgatgggtta ggaattgatg gaaagtaaga acaagctaaa gcgtgggcta
    14581 agtacccgcc acatacgctt tatggcactg ggttcagcaa ttggcaccgg gctgttttac
    14641 ggttcggcag acgccatcaa aatggccggt ccgagcgtgt tgttggccta tattatcggt
    14701 ggtatcgcgg cgtatatcat tatgcgtgcg ctgggggaaa tgtcggtaca taacccggcc
    14761 gccagctctt tctcgcgtta tgcgcaggaa aacctcggcc cgctggcagg ttacattacc
    14821 ggctggacct actgctttga aatcctaatt gtcgccatcg ccgatgtgac cgcttttggt
    14881 atctatatgg gtgtctggtt cccgacggtg ccgcactgga tttgggtact gagcgtggtg
    14941 ctgatcattt gcgccgtaaa cctgatgagc gtgaaggtat tcggtgagct ggaattctgg
    15001 ttctcgttct ttaaagtcgc caccatcatc atcatgattg tcgctggttt cggcatcatc
    15061 atctggggga ttggcaacgg cgggcaaccg accggtattc ataacctgtg gagcaacggc
    15121 ggcttcttca gtaacggctg gcttgggatg gtgatgtcgt tgcaaatggt gatgtttgct
    15181 tacggtggga tcgaaattat cgggattacc gccggtgaag cgaaagatcc tgagaaatcg
    15241 ataccgcgtg cgattaactc cgtgccgatg cgtattctgg tgttctacgt cggtacgctg
    15301 ttcgtcatta tgtctatcta cccgtggaat caggttggca ctgccggtag cccgttcgtg
    15361 ctgacgttcc agcatatggg cattaccttt gccgccagca ttcttaactt tgttgtgctg
    15421 actgcttcgc tgtcggcaat taacagtgac gtatttggcg taggccgtat gctccacggt
    15481 atggcagagc agggcagcgc accgaaaatt ttcagcaaaa cgtcgcgtcg cggtattccg
    15541 tgggttacgg tgctggtgat gactaccgcg ctgctgtttg cggtgtatct gaactacatc
    15601 atgccggaaa acgtcttcct ggtgatcgct tcgctggcaa ccttcgccac ggtgtgggtg
    15661 tggattatga tcctgctgtc gcaaattgcc ttccgtcgcc gtttgccgcc agaagaagtt
    15721 aaggcgctga aatttaaagt gccgggtggg gtagcaacga ccatcggcgg gctgattttc
    15781 ctgctcttta ttatcgggtt gattggttat cacccggata cgcgtatctc gctgtatgtc
    15841 ggtttcgcgt ggattgttgt gctgttgatt ggctggatgt ttaaacgccg ccacgatcgt
    15901 cagctggctg aaaaccagta atacttgcgt tctgtaagcc ggttcaggag gaaatcctga
    15961 tccggctatt ccgaaagtta tccgcccccg tcctcctccc ccaaatatcc ttcagatgat
    16021 gagtgatcct gcattaggct atggcaaggt gatcagattt tcatcacagg ggaattatga
    16081 tgttaaatgc atggcacctg ccggtgcccc catttgttaa acaaagcaaa gatcaactgc
    16141 tcattacact gtggctgacg ggcgaagacc caccgcagcg cattatgctg cgtacagaac
    16201 acgataacga agaaatgtca gtaccgatgc ataagcagcg cagtcagccg cagcctggtg
    16261 tcaccgcatg gcgggcagcg atcgatctct ccagcggaca accccggcgg cgttatagtt
    16321 tcaaattgct gtggcacgat cgccagcgtt ggtttacacc gcagggcttc agccgaatgc
    16381 cgccagtacg actggagcag tttgccatcg atgtaccgga tatcggccca caatgggcag
    16441 cggatcagat tttttatcag atcttccctg atcgtttcgc ccgtagtctt cctcgtgaag
    16501 ctgaacagga tcatgtctat taccatcacg cagccggaca agagatcata ttgcgtgact
    16561 gggatgaacc ggtcacggcg caggcgggcg gatcaacgtt ctatggcggc gatctggacg
    16621 ggataagcga aaaactgccg tatctgaaaa aacttggcgt gacggcgctg tatctcaatc
    16681 cggtgtttaa agctcccagc gtacataaat acgataccga ggattatcgc catgtcgatc
    16741 cgcagtttgg cggtgatggg gcgttgttac gtttgcgaca caatacgcag cagctgggaa
    16801 tgcggctggt gctggacggc gtgtttaacc acagtggcga ttcccatgcc tggtttgaca
    16861 ggcataatcg tggcacgggt ggtgcttgtc acaaccccga atccccctgg cgcgactggt
    16921 actcgtttag tgatgatggc acggcgctcg actggctggg ctatgccagc ttgccgaagc
    16981 tggattatca gtcggaaagt ctggtgaatg aaatttatcg cggggaagac agtatcgtcc
    17041 gccactggct gaaagcgccg tggaatatgg acggctggcg gctggatgtg gtgcatatgc
    17101 tgggggaagc gggtggggcg cgcaataatt tgcagcacgt tgccgggatc accgaagcgg
    17161 cgaaagaaac ccagccggaa gcgtatatag ttggagagca ttttggcgat gcacggcaat
    17221 ggttacaggc cgatgtggaa gatgccgcca tgaactatcg cggcttcaca ttcccgttgt
    17281 ggggatttct tgccaatacc gacatctctt atgatccgca gcaaattgac gcccaaacct
    17341 gtatggcctg gatggataat taccgcgcag ggctttctca tcaacaacaa ttacgtatgt
    17401 ttaatcagct cgacagccac gatactgcgc gatttaaaac gctgctcggt agggatgttg
    17461 cgcgcctgcc cctggcggtg gtctggctgt tcacctggcc tggtgtaccg tgcatttatt
    17521 acggtgatga agtgggactg gatggtaaaa acgatccgtt ttgccgtaaa ccgttcccct
    17581 ggcaggtgga aaagcaggat acggcgttat tcgcgctgta ccagcgaatg attgtgctgc
    17641 gtaagaaaag tcaggcgctg tgtcgtggcg gctgtcaggt gctgtatgcg gaagataacg
    17701 tggtggtatt tgtccgcgtg ctgaatcagc agcgtgtact ggtggcaatc aaccgtggtg
    17761 aagcctgtga agtggtgcta cccgcgtcac cgtttcttaa tgtcgcgcaa tggcaacgca
    17821 aagaaggcca tgggcaactg acagaaggga ttctggcttt gcctgccatt tcggctacgg
    17881 tgtggatgaa ctaaagcatc tgttttattt gttacgtcgt agtcaccagc cctgacacta
    17941 cgcagcgaaa aagctgcaca atgtcagggc tgagtttgtt aaatatttgt cccgatcgat
    18001 atgagcgatt tttggtattc tttgcccatg aggttatgta caacatgctc caggataacc
    18061 ctgtagtgga gcaaagatat gacaatttca aattcgaatt tattaaaacc gtttattact
    18121 ggttctaagt tgctaccttt taaggatgat gtcttttcta aaacagaaga acaatttaaa
    18181 aaaagccccc atggcaaatc gcttttcatt tccggaaatg aaaacttagt aaacatttca
    18241 gcggaaaaaa tcgatgaaat aaa
//
LOCUS       DAZFFG010000092        17771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFFG010000092 DAZFFG010000000
VERSION     DAZFFG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.092"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1582)
                     /gene="lsrK"
                     /locus_tag="ACHJ7J_003575"
     CDS             complement(<1..1582)
                     /gene="lsrK"
                     /locus_tag="ACHJ7J_003575"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO5001759.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELSVDPSNAGTTGLLDLTIRDWKPSLLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKA"
     gene            complement(1661..2614)
                     /gene="lsrR"
                     /locus_tag="ACHJ7J_003576"
     CDS             complement(1661..2614)
                     /gene="lsrR"
                     /locus_tag="ACHJ7J_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO5001760.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2863..4398
                     /gene="lsrA"
                     /locus_tag="ACHJ7J_003577"
     CDS             2863..4398
                     /gene="lsrA"
                     /locus_tag="ACHJ7J_003577"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO5001761.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4392..5420
                     /gene="lsrC"
                     /locus_tag="ACHJ7J_003578"
     CDS             4392..5420
                     /gene="lsrC"
                     /locus_tag="ACHJ7J_003578"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO5001762.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5420..6412
                     /gene="lsrD"
                     /locus_tag="ACHJ7J_003579"
     CDS             5420..6412
                     /gene="lsrD"
                     /locus_tag="ACHJ7J_003579"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO5001763.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINSRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGRFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6424..7446
                     /gene="lsrB"
                     /locus_tag="ACHJ7J_003580"
     CDS             6424..7446
                     /gene="lsrB"
                     /locus_tag="ACHJ7J_003580"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO5001764.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7473..8348
                     /gene="lsrF"
                     /locus_tag="ACHJ7J_003581"
     CDS             7473..8348
                     /gene="lsrF"
                     /locus_tag="ACHJ7J_003581"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO5001765.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8372..8662
                     /gene="lsrG"
                     /locus_tag="ACHJ7J_003582"
     CDS             8372..8662
                     /gene="lsrG"
                     /locus_tag="ACHJ7J_003582"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO5001766.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8719..9477
                     /gene="tam"
                     /locus_tag="ACHJ7J_003583"
     CDS             8719..9477
                     /gene="tam"
                     /locus_tag="ACHJ7J_003583"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO5001767.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMVAEARSALPDCQFVEADIRNWQPEQTLDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCKVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9481..10395)
                     /gene="yneE"
                     /locus_tag="ACHJ7J_003584"
     CDS             complement(9481..10395)
                     /gene="yneE"
                     /locus_tag="ACHJ7J_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO5001768.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10592..12043)
                     /gene="uxaB"
                     /locus_tag="ACHJ7J_003585"
     CDS             complement(10592..12043)
                     /gene="uxaB"
                     /locus_tag="ACHJ7J_003585"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO5001769.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12270..13688)
                     /locus_tag="ACHJ7J_003586"
     CDS             complement(12270..13688)
                     /locus_tag="ACHJ7J_003586"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5001770.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13827..14186)
                     /gene="yneG"
                     /locus_tag="ACHJ7J_003587"
     CDS             complement(13827..14186)
                     /gene="yneG"
                     /locus_tag="ACHJ7J_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO5001771.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14186..15112)
                     /gene="glsB"
                     /locus_tag="ACHJ7J_003588"
     CDS             complement(14186..15112)
                     /gene="glsB"
                     /locus_tag="ACHJ7J_003588"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO5001772.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15176..16564)
                     /gene="sad"
                     /locus_tag="ACHJ7J_003589"
     CDS             complement(15176..16564)
                     /gene="sad"
                     /locus_tag="ACHJ7J_003589"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO5001773.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     YGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16665..17546
                     /gene="yneJ"
                     /locus_tag="ACHJ7J_003590"
     CDS             16665..17546
                     /gene="yneJ"
                     /locus_tag="ACHJ7J_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5001774.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17624..>17771
                     /gene="yneK"
                     /locus_tag="ACHJ7J_003591"
     CDS             17624..>17771
                     /gene="yneK"
                     /locus_tag="ACHJ7J_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO5001775.1"
                     /translation="MVTPVSISNYICLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTS"
BASE COUNT         4423 a         4552 c         4454 g         4342 t
ORIGIN      
        1 gcgctttcca taacgacgtc gtcagtccat gatcaaccag ccccagctga tcctgataaa
       61 ctgcctgcca cttatcgcgt gaatcctgat aaagttcatg cttttccggg tctggtgtgt
      121 gcgtccgttc ccagcgaacc aggcgttctc cggtttctgc cattgatgaa aaaattccgg
      181 caccgacgcc agctgcaatg gcacatccta atgcagtggc ttctttgacc accggaatat
      241 tgacgggtaa tcccgagaca tcagcgagaa tttgactcca taatttccct tttgaacctc
      301 cgcctgcaaa gactaacgat gaaggatgaa tattcgaaaa atcagcaatt tgctgcaagt
      361 tacacgctga tacaatcgcc gcattttctt ccagcgcacg gaacaatgtc gctttgttac
      421 atttatccgg gtcaatggac aaattaataa aggaaggcgc agcgtgatac caggttttaa
      481 agcgcattct gtcggagaag atcggcatta cgccccacga cccaggcggt acccgactgg
      541 ccatctcttc cagcagcgta taggtgtcga tgcctaaacg ttcggcaatc agtttttctt
      601 cggcacagaa agcatcgcgg aaccagcgca tggtgagtcc ggtaaaaaag cttatagatt
      661 cagcttgtac catgccagga ataacatgag gattaacgcg cacgttcatt tctgggtctg
      721 tcaccggcgc ggctaaattt acaacttgct gccagaatgt gccgccaaga accgcggttt
      781 gtgccggacg cacaacgcct aaccccaggc aaccaagctg cacgtcgcct cctccaacga
      841 ccaccggagt gcccaccttc agaccgcaga gttccgccgc ttgtgaactt accacgccca
      901 gcaatgtgcc ggtttctttg acaggagaaa gaatatcggc acgcaggcca gccatatcca
      961 gcaatgaagg tttccagtca cggatggtta gatcaagaag tcccgtggtg ccagcgttag
     1021 agggatccac cgaaagttcg ccgctgagca tataggccag ccagtcgctg atcatggtga
     1081 tggttgatgc ctgacggtaa atatcggaac gatggtgcgc cagccaaagt aatctgggga
     1141 tggcacttaa agccagtgtt tgtccggtcg cgcgataaac ttcgttttca aaggtattgt
     1201 tgtgcagttc tttaagttcg ctaacttcgc gcgccgctct ggcatccaca ttggcgcagg
     1261 cccagatcgg tgttccttca ttgttatata aaacaatgcc ttcacgcatc gaacatgccg
     1321 aaacggcagc gatatactcc ggggctatgc cggcgttgtg caacgcctgg cgcatacact
     1381 cacacgccag ttgccagttt ttgttgagat caaattccat agaaccagga acgtccggta
     1441 ctgccagatg ccgccactcc gcctgtccca ctgctatttg attgccttct aggtcgaata
     1501 tcacagcccg aatacttccg gtgcctgcat ccagcgccat caggtagtac tttgattctg
     1561 aaggggtaaa gagtcgagcc attagattat cctcggctta tgcgttctgt ctgttcctct
     1621 atacgttctc catcattccc ggtaataagg tcatgcaaat ttaactacgt aaaatcgccg
     1681 ctgctgtgtc ctgatcggta accagtgcgt tgatataacc gcctttcatt gcagcggcaa
     1741 ttgcttcggc tttattttct ccccctgcca cgccaacccg gacgggtatg gtcttcagcg
     1801 cgcttaaagg taagccaatc agttcgttat gtattttaat atccgtgaca acgtcacctt
     1861 ttgcatcaaa aaagtagcct aaaatgtcgc caaccgcccc ttttcggcca atcattaact
     1921 gttcgccctg gctgatataa ccggagcgaa tgatcgtcgc gtcgtcttgt tgactaacgg
     1981 ccccaatgcc gacaatcgcc acatccgctg cttgcgcggc taacagaaca tctttgacgc
     2041 aattttcatt ttttagcgta cgggcaatgt cagcggagga tgcccgcaac ggagccggaa
     2101 taatattcac actgcacgcc gcattaagct gtccgatacc cgtcatataa gaaccgacgc
     2161 caccggagag cgtgaccagg cgaatttgct gtgacgaaat aaaaccactt aagcgttgca
     2221 gcgtattcat ggttgcctcg ccaaaaccaa tcgccagcat ctgttgtggt tgaagtaaac
     2281 tcatcaacat atgcgccgcg cctatcccca gtcgcccgcc gacatcagca tccgcaagcc
     2341 cagggatcac tcggacatgt tgcagcgaaa actgacgacg taattgtgtt tcatattcca
     2401 gacagccttc aaagcgagaa ttaatctgca cgcgaataat gccggactga tgccctttct
     2461 ccagcaatcg cgacactttc aaacgtgtca ggccgagacg atcgctgatc tcactctggg
     2521 tcagcccgtc gtggtagtaa aaccacgcga tccgcgcgac ctgttcttct tcacacattc
     2581 cctgttctga aattgccgaa tcgttgattg tcataattca ttcttcactt tgaacatatt
     2641 taaatcttta atgcaattgt tcagttcttg cgcatttata tctgtgatgg caaccacagt
     2701 ttgactctac aagtatgaac aaacgcaacc gtgaaaatca aaatagcata aattgtgatc
     2761 tattcgtcgg aaatatgtgc aatgtccacc taaggttatg aacaaattaa aagcagaaat
     2821 acatttgttc aaaactcacc tgcaaaactg aacgggggaa atatgcaaac gagtgatacc
     2881 cgcgcgttac cgctactttg cgcccgctcg gtttataaac agtattcagg ggtcaatgtc
     2941 ctgaaaggca tcgattttac gttgcaccag ggggaggtcc acgccctgct cggcggcaat
     3001 ggtgccggta aatcgacgtt aatgaagatt attgccggta ttacccctgc tgatagcggt
     3061 acgctggaga ttgggggcaa caactacgtc agattaacgc cagttcatgc tcatcagctg
     3121 ggtatttatc tcgttcccca ggaaccgctg cttttcccaa gcctgtcgat aaaagaaaac
     3181 atcctgtttg ggctggcaaa aaaacagctc tccatgcaga aaatgaagaa cttgctggcg
     3241 gcgctgggct gccagtttga tctgcatagt ctggcaggat cgctggatgt cgccgatcgc
     3301 caaatggtgg aaatcctccg cgggctgatg cgcgactcgc ggattctgat cctcgatgaa
     3361 cctaccgcct cgctcacccc tgcggaaacc gaacgcttgt ttactcgctt gcaagagctg
     3421 cttgctactg gcgtgggtat tgtttttatc tcgcataagc tgccggaaat tcgccagatt
     3481 gccgatcgaa ttagcgtgat gcgcgacgga accatcgcct taagcggcaa aaccagcgaa
     3541 ctgtctaccg acgacattat tcaggccatc accccagcgg tacgggaaaa atcgctctct
     3601 gccagccaaa aattatggct ggagttacct ggtaaccgcc cacaacatgc cgccggaacg
     3661 ccggtgctga cactggaaaa tctgactggc gaaggtttca ggaatgtcag cctgacgctc
     3721 aatgccggag aaattctggg cctggctggg ctggtgggtg ccggacgcac agaactggcc
     3781 gagacgctct atggtctgcg tactttgcgt ggcggacgca ttatgctgaa tggtaaagag
     3841 atcaatagat tatccactgg agaacgttta ctgcgcggtc tggtttatct gccggaagat
     3901 cgccagtcat ccggactgaa tctcgatgct tcgctggcct ggaacgtctg cgcccttact
     3961 cataaccttc gtggattctg ggcgaaaacc gcgaaagata atgccacact ggaacgatat
     4021 cgtcgggcgc tgaatattaa atttaaccaa ccggaacaag ctgcacggac attatccggt
     4081 ggcaaccagc aaaaaatcct gattgccaaa tgcctggaag cctcgccgca agtattgatt
     4141 gtcgatgagc cgacgcgcgg cgtggatgtc tcggcgcgta atgatatcta ccaactgttg
     4201 cgcagcatcg ccgcacagaa tgtggctgtg ctgcttatct cctccgacct ggaagagatc
     4261 gaactgatgg cagatcgtgt gtatgtgatg catcagggcg aaattaccca ctctgcactg
     4321 accgggcgcg atattaatgt cgagactatt atgcgcgttg ccttcggcga tagtcagcgt
     4381 caggaggcgt catgctgaag tttattcaga acaaccgtga aatcacggca ctgctggcgg
     4441 tggtgctgct gtttgtatta cccggttttc tcgaccgcca gtatttaagt gtgcaaacgc
     4501 tgaccatggt ttatagcagc gcgcaaatcc tgatcctgct ggcaatgggc gcgacgctgg
     4561 taatgcttac gcgcaatatt gatgtttcag tgggttcgat taccggaatg tgcgcggtgc
     4621 tgttggggat gttactgaac gcaggatatt cactacctgt tgcctgtgtc gcgactttac
     4681 tgcttggttt gctcgcggga tttttcaacg gtgtcctggt cgcgtggcta aagatccctg
     4741 ccattgttgc cacccttggc acgttagggt tgtacagagg catcatgttg ctgtggactg
     4801 gcggcaaatg gattgaaggg ttacccgccg aactgaaaca gctctccgcc ccgctgctgc
     4861 ttggcgtttc agcaattggt tggttgacga taattctggt ggcatttatg gcctggctgc
     4921 tggcgaagac ggcgtttgga cgcagttttt atgccacggg cgataattta cagggcgctc
     4981 gtcaactggg cgtccgtacg gaagccattc gcattgtggc attttcgttg aacggctgca
     5041 tggcggcact ggcggggatt gtgtttgctt cgcagattgg ttttatcccc aaccagaccg
     5101 gtaccgggct ggagatgaaa gcaattgcag cctgcgtgct gggcggcatt agtttgctcg
     5161 gcggttccgg tgcgatcatc ggtgcggtac tcggcgcatg gttcctgacg cagatcgata
     5221 gcgtactggt gctgttgcgc attccggcat ggtggaacga ttttatcgcg ggcctcgtgc
     5281 tgctggcggt gctggtgttt gatggacgcc tgcgttgtgc gctggaacgt aatctacggc
     5341 ggcaaaaata tgcccgcttt atgacgccac cgccatccgt taaacccgct tcgtcaggta
     5401 aaaaacggga ggccgcataa tgcgtattcg ctacggttgg gaactggctc ttgccgcact
     5461 gctcgttatt gagattgtcg catttggtgc aattaactcg cggatgttag atctcaatat
     5521 gttgctgttc agcaccagtg actttatctg cattggcatt gtcgccctac cgctgacgat
     5581 ggtgattgtc agtggcggga tcgatatttc gtttggttcg accatcggcc tctgcgccat
     5641 tgcattgggc gtactgtttc aaagtggtgt gccgatgccg ctggcgatac tcctgacctt
     5701 actgctcggc gcattgtgcg ggctgatcaa cgccggatta attatctata ccaaagttaa
     5761 cccgctggtg attacgcttg gcacgctgta tctgtttgct ggaagcgctc tgctgctttc
     5821 cgggatggcc ggagcgacgg ggtacgaagg tattggtaga ttcccgatgg cgtttacaga
     5881 tttcgctaac ctggatgtgc tgggactccc cgttccgctg attatcttcc tgatatgtct
     5941 cctcgttttc tggctctggc tgcataaaac ccatgccgga cgtaatgtgt ttttgattgg
     6001 gcaaagcccg cgcgtggcgc tttatagcgc gattccagtt aaccgcacct tatgtgcgct
     6061 ctatgccatg acggggctgg cgtctgcggt cgccgctgtg ctgctggtat cgtattttgg
     6121 ttcagcacgt tccgatctcg gtgcgtcgtt tctgatgccc gccatcaccg ccgtggtgct
     6181 tggcggggcc aatatttatg gtggttccgg ttccattatc ggcaccgcca ttgcggtttt
     6241 attagtggga tatttgcaac aaggtttgca aatggcagga gtgccaaatc aggtgtccag
     6301 cgccctttcc ggtgcgctac ttatcgtcgt tgtcgtaggt cgttccgtta gcctgcatcg
     6361 ccagcaaatt aaagagtggc tggcgcgtcg ggccaataac ccattgccat aaaggatatc
     6421 ttcatgacac ttcatcgctt taagaaaatc gccttactta gcgctcttgg cattgccgca
     6481 atctctatga atgtgcaggc cgcagagcgt attgcattta ttcccaaact ggttggcgtg
     6541 ggatttttta ccagcggtgg caacggcgca caacaagcgg gtaaagagct gggcgttgat
     6601 gtgacctacg acgggccgac agaacccagt gtttctggtc aggtacagtt gattaataac
     6661 ttcgtcaatc aaggttataa cgccattatc gtttctgcgg tttcgcctga tggcttgtgt
     6721 ccggcactga aacgcgccat gcaacgtggt gtgagagtgc tgacctggga ctctgataca
     6781 aaaccggagt gccgctctta ctacattaat cagggaacgc ccgcccagtt gggaggtatg
     6841 ttggtggata tggcggcgcg tcaggtgaat aaagacaaag ccaaagtcgc gtttttctac
     6901 tcaagcccca ccgttacgga ccaaaaccag tgggtgaaag aagcgaaagc gaaaatcgcc
     6961 aaagagcatc ctggctggga aattgtcact acgcagtttg gctataacga tgccactaaa
     7021 tcgttacaaa ccgcagaagg aatattaaaa gcgtatagcg atctcgacgc cattatcgcc
     7081 cccgatgcca acgccctgcc cgctgccgca caagccgcag aaaacttgaa aaatgacaaa
     7141 gtagcgattg tcggattcag tacgccaaat gtgatgcgcc cgtatgtaga gcgcggcacg
     7201 gtgaaagaat ttggcctgtg ggatgtggtt cagcaaggca aaatttcagt ttatgtcgcg
     7261 gatgcattat tgaaaaaagg atcaatgaaa acgggcgaca agctggatat tcagggcgta
     7321 ggtcaggtgg aagtctcgcc aaacagcgtc cagggctatg actacgaagc ggatggtaat
     7381 ggcatcgtac tgttaccgga gcgcgtgata ttcaacaaag agaatatcgg caaatacgat
     7441 ttctgatgtg cattacttaa ccggagtaag ttatggcaga tttagacgat attaaagatg
     7501 gtaaagattt tcgtaccgat caaccgcaaa aaaatatccc ttttaccctg aaaggttgcg
     7561 gtgcgctgga ttggggaatg cagtcacgct tatcgcggat atttaatccg aaaacgggta
     7621 aaaccgtgat gctggccttt gaccatggtt attttcaggg gccgactacc ggacttgaac
     7681 gcattgatat aaatatcgcc ccgctgtttg aacatgccga tgtattaatg tgtacgcgcg
     7741 gcattttgcg cagcgtagtt cccccggcga ccaataagcc ggtggtactg cgggcgtcag
     7801 gtgcgaactc tatcctggcg gaattaagca atgaagccgt ggcgttatcg atggatgacg
     7861 ccgtgcgcct gaacagttgc gcggtggcgg cgcaggttta tatcggcagc gaatatgaac
     7921 atcagtcgat caaaaatatt attcagctgg tcgatgccgg aatgaaagtg ggaatgccga
     7981 ccatggccgt gactggcgtg ggcaaagaca tggtgcgcga tcagcgttat ttctcgctcg
     8041 cgactcgaat cgccgctgaa atgggggcgc aaattatcaa aacctattat gtcgaaaaag
     8101 gttttgaacg gattgttgcc ggatgtccgg tacccattgt tattgctggc ggtaaaaaat
     8161 taccggagcg cgaggcgctg gaaatgtgct ggcaggctat cgatcagggc gcttctggtg
     8221 tggatatggg gcgtaatatt ttccagtctg accatccggt ggcgatgatg aaagccgtac
     8281 aggcggtggt acaccataac gaaacggctg atcgggcata tgaactctat ctgagtgaaa
     8341 aacagtaact gcggatttaa ggagaagaat tatgcacgtc acactggttg aaattaacgt
     8401 tcatgaagac aaggttgacg agtttatcga agtttttcgc cagaaccacc tgggctctgt
     8461 acaggaagaa ggcaatttgc gcttcgatgt cttacaggac ccggaagtga attcgcgctt
     8521 ttatatctac gaagcctata aagatgaaga cgcagtggcg ttccataaaa ccacgcccca
     8581 ctacaaaacc tgtgtcgcga aactggaatc tttaatgacc gggccgcgta aaaaacgtct
     8641 gttcaatggg ttgatgccgt gaggcgaatt tatcagtttt atctacaatt ggggtaacgc
     8701 gctgacggga gtaaataaat gtctgactgg aacccctctt tatatctgca ctttgccgct
     8761 gaacgatcgc gtccggcggt ggagctgctt gccagagtgc cgctggagaa tatcgaatat
     8821 gttgccgatc ttggctgtgg cccaggtaac agcaccgccc tgctgcatca acgttggcct
     8881 gctgccagga taacgggcat cgactcatct ccggcgatgg ttgccgaagc acgcagtgct
     8941 ttgccggact gccagtttgt ggaagcagat atccgcaatt ggcaaccgga acagacactc
     9001 gatctgattt ttgctaatgc ctcactgcaa tggctgcccg accactacga attgtttcct
     9061 catctggttt cgttacttaa tccgcagggc gtgctggcag tacagatgcc agataactgg
     9121 ctggagccga cccatgtgct catgcgcgaa gttgcctggg aacaaaacta cccggatcgc
     9181 gggcgtgagc cgctggcagg cgttcatgct tactacgata ttttgagcga agccggatgt
     9241 aaggtcgata tctggcgaac cacttactat catcagatgc cgtcgcacca ggcgattatc
     9301 gattgggtga ccgccactgg attacgtccg tggttacagg atctgaccga gagcgaacag
     9361 cagcattttc ttacgcgcta ccatcagatg ttggaagagc agtatccact gcaagagaac
     9421 ggacagatac tgctggcatt tccgcgtctg tttattgttg cccggcgtac ggagtaagta
     9481 tcacgtcagc tggtaatgac gatcgggaag aatttttgct ggaatttcgg cttcatcgtt
     9541 catctgtaag aggtcaattt caatagcgtt gcagatggca tccagtggta aatcattgtt
     9601 ttcagtaccg aatggatctt ccagttcttc cgccagacaa tccagcgaaa taaaagtgta
     9661 ggaaatcagc acagagataa aaggcgtcat gtaatgcagg tccacgacca gcgcgaacgg
     9721 cagcatgata caaaacagat aaacggtacg atgcaaaatc agggtgtagg caaagggaat
     9781 tggcgtgtag gcaatgcgct cgcagcctgc cagaaccgct gaaatatcgt taagccgatc
     9841 gttgaggcta ataaacagga tatctgaaag ctgtccattg cggcgctgaa ccgccaacca
     9901 ctcccccatt attaacaaga tgcggttagc tggagagttc gaagccagta cacgctgaag
     9961 atcttcagtc ttgagataat gagccagcac ttccgcctgt ggctgtttgc gtaatgtcat
    10021 gcgtaaacag tgggcgaagg cgatttgcag ccgggcaaac tcccttacac ttgccgaatc
    10081 cggcaatgtc gtttttacct cgcgcagtaa cgaccgtgag gcaatcatca actgccccca
    10141 aagtttgcgc gcttcaacgt aacgggcgta accggcatta ttacgaaaac caagaaaaat
    10201 ggcgatggcg acaccgagaa tgctgaacgg tgcgagggtg aatttgatgc ccagatgcgt
    10261 gtaccagggc agcatgaaaa taacagcgat agaaaagaga aaattgagta gtaagcgcga
    10321 ggatatcttg gataatactg agccatgcca gacaaaaata cggcgcagcc agtgttgttg
    10381 tggacgaaca atcatggtta tcttcaggcg tggaaaagtc gccctattaa acgtgattac
    10441 gatcacattc tcaagacgct acttacaaat tacctacaaa attacaacat taacaggcgc
    10501 tgcatgtggc acctgttaat gataaaggct tataggatgt tgtaactaat ataatgaaat
    10561 aagaaacccg gtcgaaaccg ggttcagagt attagcacaa cggacgtaca gcttcgcgca
    10621 tccctttttc gagaatcgca tccaggtcat tagccacctg ctcgaccaga ccaggcactt
    10681 gcgtcaggtc ctgctcccag tggtcttttt ctgccaacac aatcgctacc agttcctggg
    10741 taccgataac acggtcacga tgctgactcc agagttgctg ataacgttcc agccagtgtg
    10801 catcgtcctg taccggatat gtttctccat tacgctcacc gcgatagaac gcaatcaatg
    10861 ccgctaatgc gaaagtaagg cgcgccggaa gtgtgccgtt tgccttctgc cctgccagca
    10921 gctgcggcag gatgcgggta cggaacttgg tcataccgtt gagcgcgata gacagcagct
    10981 gatgcttaat gtacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
    11041 catcgcgtgg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
    11101 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
    11161 ccggcaccaa cgcggtgtgt gcaccgttga gtattgccac tttgcgttct ttatacggtt
    11221 taatatcgtc aacaatcagc acgttgagcg gatatttgtc cagacgcagc tcggtcgcta
    11281 aggatttcgg tccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
    11341 cgtgataacc cagttcttct tccagtttag ccacttcatc acgcggatag ccggtaacga
    11401 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
    11461 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
    11521 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
    11581 taaaacgttc gaacagcaga cgggtcagtt ttgccggata acttaccgct ggcgcgtcat
    11641 cgaatttatc gcccgcatgg tagctgatac ccgcttcggt ggtgttagag aaaacaaagc
    11701 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
    11761 cacgattaac agagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
    11821 cacggataat ggtggtgtac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
    11881 caatcggacg aacaacgacc acaccagaat tcagatcggt gtgctcattc aggagatcta
    11941 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
    12001 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
    12061 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
    12121 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
    12181 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
    12241 attcaaatcg ctctgaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
    12301 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
    12361 aatttattaa agtcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
    12421 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
    12481 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
    12541 aattcttcgc cgcccattcg cgccaccagc cccttatcac cgacaatctt ctggatatgc
    12601 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatactt
    12661 ttgaaatagt cgatatcaag cagcatgaca gtcagatgtt gtgtctgttt cagcgatgga
    12721 cttttcagcg cctcataaag accggaccgt gagtaaacct gagtcagaaa atcaaagtcg
    12781 gctcgcagcg caacttgctt cattagcgaa ttgatcgctg ccacgctaaa agaaaccata
    12841 attgggcata tcgccatcgt ggcaataccg agacgtgcgg agaacatttc cggaatggag
    12901 aacggcgaac cgaccgagat atcaatcacc gaattagcca ccagtacgat ttccaccgca
    12961 ccggtgacaa aggtcaacag acatgttacc tgcggcgtat agcgcactgc acaccagatt
    13021 aatgcaggca gcgggaacgc cagactcccc gctccgccaa tgactaccga ggcaataacc
    13081 gacacaataa gcgcgatggc gggcatcatc tgttctgctt taaagcgcgg cagcactcca
    13141 ggaatagcca acgtcagcat acaaggcacg atcaacacgc ccgttgagaa ttgctcactg
    13201 aaccagtccg caagcaaagg ccagaaatcc agactatcaa tactgaccga accaatcgcc
    13261 ccgacaatag cgcataataa tgcggcaatc agacagtaat taaatagccg taaggcactt
    13321 actggctcat atttattttt ccccaaacgc ttatcccgca cgaccagtaa ggcgacggta
    13381 acaataaaca tcatattgga gaaattgata gccagtgaaa ccagtcccca ttcggtggtt
    13441 atggcatcat aaaccagcat cgccacataa ctgatcgcat agtaatgcag tcgattaagc
    13501 cagacatagc gggcaaacac ccccgccatt acgccattca gcggccagaa aagagaaagt
    13561 tcgtgaacca gccgtaactc tgcaccaata aaatagaaca gcgtggttag cgcaaaaata
    13621 gcgatgctat tacgtagcag atgaccttcc tggaacacac ggaaagttga aatggggtgt
    13681 acatgcatta attatctgtc catacaattg cgggtggcaa ttatctcaac gtaataagta
    13741 aattctcata aaggatagca tgttcgctga cagcggcggc aaataaaggt accctgggaa
    13801 tgctattttg tcgttttccg caattctcat ggctgattca tctgtacaac cagccagtga
    13861 tagattaccg ccacgatata acgttgttgt tcttcgctta gcgaaacacc ctgcggaatg
    13921 ttatgccact tagccagaca accgcgacag caggtcgcgg tggcgtgctg agcgataaac
    13981 accggatgcc cgcgcatggg ggtttgctta ccgtcattag ccggtaatgc cggagcaaga
    14041 cgtttagcga caaaatccgc cgcatgttgt tcgatgactg gcgcgccttt ctccagacaa
    14101 tactgacgct ctttcatgcc caaacgaaag cgagatcgaa attttgaacg ggataaacgc
    14161 gcgaagagtg gatcgagaga ctgcattaat aaaccgaacg ccctaactgt ttcgtcaatt
    14221 gttcaaggac ggcgataccc gcaagcgagt tacctgcatc atccagttcc ggactccaga
    14281 ctgcgatggc catttcatgc ggaacaatcg ccacaatacc gccaccaacg ccagatttcg
    14341 ccggtagtcc cacccgccag gcaaactccc ccgcgttctg gtacatacca ctggtcgcca
    14401 tcagcgcgtt aatttgccgt gcctgcattg gcgtcaccac aggttcatca atatgaatag
    14461 ctttcccctg attagccaga aagacaaacg tccgggccag ctctacacag ctcattttca
    14521 gagcgcagta atgaaagtag ttttgcagaa cggttgtcac gtcatgatgg aaattgccaa
    14581 acgacttcat cagccaggcg atagccgcat ttcgcgcgga atgttcaaat tcggaacgcg
    14641 ctaccaccgt atcgtaggaa atatcagaca caccgcttaa gccgcgcacg acttccagca
    14701 tacgttgccg tggtgcgctt aatcgccctt gcaacatatc gcagaccacc agcgcaccgg
    14761 cattaataaa cggattacgc ggaataccct gctccatttc cagttgcact aaagaattga
    14821 acggtgatcc agacggatct ttgccgacgc gttgccagat ttcctcttcg gagtaatgac
    14881 gcatggcgac aacgagactc agcactttag aaatagactg aatggaaaaa cgttcttgcg
    14941 cgtctccggc ctgaaaaagc tgtccgtcaa cggtacagat agcaatgccc aatcgggaac
    15001 cgtctactgt agccagcgcc ggaatataat ccgcgacttt accctgacca atgagcggcc
    15061 gcacttgccg caagatgttt tctaaaattg cattatccat ggcgactgcc actttctact
    15121 cctggaccgc aggtctgaaa agacctgcga gtatatcaga gctgaatatg tcgcgtcaga
    15181 tccggtcttt ccacaccgtc tggatattac agaattcgtg taatccgaaa tgggaaagct
    15241 cacgaccaaa gccactcttt ttcacgccac caaaggccac tcgcgcgtcg ctggcacaat
    15301 aaccattgat aaacacccca ccgcattcca gacgtgccgc catctgtctg gcctgtgttt
    15361 cgtcggtggt aaaaatggtc gctgaaaggc cgaactcact atcattagcc agttccagcg
    15421 catgttctgc atctttcgca atggtgattg ccgcaacggg gccaaacatt tcttcccgaa
    15481 aagcggtcat ttctggggta acattcgcca gaaccgttgg cggatagtag ttacctgccc
    15541 cagccatctt ttccccgccc agtaacaaac gcgcaccctg cgccagggtt ttctccacct
    15601 gatgatgcag ctcatcacgt aaatcgaaac gagccattgg tccgagagcg ttctcttcgt
    15661 cacggggatc gcccattttc aaggctgccg cagctgccac aaaacgttcg gtaaatgccg
    15721 aagcaattcc ctcttcgata ataaagcgtt ttgccgctgc acaaacctgt ccggtattct
    15781 gataacgtcc ggctaccgcc gctttaaccg ccagttccag atcggcatcg ttaagcacaa
    15841 taaatggatc cgaaccgccc agttccagta cgcatttttt cagtgccgct ccagcctgtg
    15901 caccaatagc cgctccagca cgaacacttc cggtcaccgt gatagcagca atgcgcgagt
    15961 ctttaatcat ctgactgaca ccgtcattgt cggcattcag ccagccatat acgccttgtg
    16021 ggattcccgc atctttaaac acctgggcaa tgagctgagc acagcccatc acattcggcg
    16081 catgtttaag taagtaaccg ttaccagcaa gaatgatggg aacagcgcca cgcatcacct
    16141 gccataacgg aaaattccac ggcataatcg ccagaatcgt ccccaacggt cgatactcaa
    16201 taacagcctg ctgattttcc accagcgtag gttccgcctt cagcattgcc ggaccatatt
    16261 ctgcatacca gtcacacaaa ttcgccgatt tcgccacttc agcgcgcgcc tggttgattg
    16321 gtttgcccat ttcgcgggtg atcatttgcg ccatttcttc gctacgggcg cgcagagcct
    16381 taccgatatc acgcagtttt tcagcacgat aatctatatt tgtctcgcgc cagtcacgaa
    16441 agcctgctgc cgccagctga agtgcgtttt cgatatcatc agcgccagcc cacggcagca
    16501 cagaaagttg ttcacccgtg gcaggattta tcgaaattgc atgagttgcc ggagtaatgg
    16561 tcatcggggt atctccttta tgagtcatgg tatgaagata cgcagattta ctcttgcttt
    16621 aaaatgaata atattaagcc acttattcac gaatcgagaa tattatggat ctgacccaac
    16681 tggagatgtt caacgcggtt gccgaagctg gcagcattac gcaggctgca gcaatagtgc
    16741 atcgcgtgcc gtcgaatctc actacccgtc tacgccagct ggaaacagaa ctgggggtag
    16801 atctgtttat tcgcgagaat cagcgtttac gtctatctcc tgccgggcat aactttttac
    16861 gctatagcca acaaattctc gcgttagtgg atgaagcgcg gagcgttgtc gctggcgatg
    16921 agccgcaagg tttattttct ctgggttcgc tggaaagcac cgcagcagtt cgcattccgg
    16981 ccacgctggc ggaattcaac cgtcgttatc ccaaaattca gttttcactt tccaccggcc
    17041 cttccggcac tatgctggaa ggtgtgctgg agggaaaact taatgcggcg tttattgatg
    17101 gacccattaa ccatactgcc attgacggga taccggtata ccgcgaggag ctgatgatcg
    17161 tcacgccaca aggtcatgcg ccagtaatcc gtgccagtca ggttaatggc agtaacattt
    17221 atgccttccg cgccaattgt tcgtatcgtc gccacttcga gagctggttt catgctgacg
    17281 gtgccgctcc gggaactatc catgagatgg agtcttatca cggaatgttg gcctgtgtgg
    17341 tcgcaggagc aggtattgcg cttattccgc gctctatgct ggaaagtatg ccgggtcatc
    17401 accaggttga agcgtggccg ttagctgagc aatggcgttg gctaacaacc tggctggtct
    17461 ggcgtcgtgg tgcgaaaacg cgtccgctcg aggcatttat tcaactactg gatgtgcctg
    17521 actcgacaaa acagggttac caatgagcta tttttgatag ttcttgcggc taatatgctc
    17581 tatatagtga tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag
    17641 catcagcaat tacatatgtc ttcctgatga ttttcccgtt cgtaatattg caccgcaggt
    17701 aaaagaagtt ttaaaggatt ttattgatgc acttagtaca ataatatgta atgaagagtg
    17761 gcgcacgtct t
//
LOCUS       DAZFFG010000093        17488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFFG010000093 DAZFFG010000000
VERSION     DAZFFG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.093"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(217..1245)
                     /gene="epmB"
                     /locus_tag="ACHJ7J_003592"
     CDS             complement(217..1245)
                     /gene="epmB"
                     /locus_tag="ACHJ7J_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5001776.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1287..1853
                     /gene="efp"
                     /locus_tag="ACHJ7J_003593"
     CDS             1287..1853
                     /gene="efp"
                     /locus_tag="ACHJ7J_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5001777.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1905..2030
                     /gene="ecnA"
                     /locus_tag="ACHJ7J_003594"
     CDS             1905..2030
                     /gene="ecnA"
                     /locus_tag="ACHJ7J_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5001778.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2141..2287
                     /gene="ecnB"
                     /locus_tag="ACHJ7J_003595"
     CDS             2141..2287
                     /gene="ecnB"
                     /locus_tag="ACHJ7J_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5001779.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2463..2780
                     /gene="sugE"
                     /locus_tag="ACHJ7J_003596"
     CDS             2463..2780
                     /gene="sugE"
                     /locus_tag="ACHJ7J_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5001780.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2777..3310)
                     /gene="blc"
                     /locus_tag="ACHJ7J_003597"
     CDS             complement(2777..3310)
                     /gene="blc"
                     /locus_tag="ACHJ7J_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5001781.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3399..4532)
                     /locus_tag="ACHJ7J_003598"
     CDS             complement(3399..4532)
                     /locus_tag="ACHJ7J_003598"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5001782.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQINLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4595..4954)
                     /gene="frdD"
                     /locus_tag="ACHJ7J_003599"
     CDS             complement(4595..4954)
                     /gene="frdD"
                     /locus_tag="ACHJ7J_003599"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5001783.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4965..5360)
                     /gene="frdC"
                     /locus_tag="ACHJ7J_003600"
     CDS             complement(4965..5360)
                     /gene="frdC"
                     /locus_tag="ACHJ7J_003600"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5001784.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5371..6105)
                     /gene="frdB"
                     /locus_tag="ACHJ7J_003601"
     CDS             complement(5371..6105)
                     /gene="frdB"
                     /locus_tag="ACHJ7J_003601"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5001785.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6098..7906)
                     /gene="frdA"
                     /locus_tag="ACHJ7J_003602"
     CDS             complement(6098..7906)
                     /gene="frdA"
                     /locus_tag="ACHJ7J_003602"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5001786.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8231..9208
                     /gene="epmA"
                     /locus_tag="ACHJ7J_003603"
     CDS             8231..9208
                     /gene="epmA"
                     /locus_tag="ACHJ7J_003603"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5001787.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9427..10929
                     /gene="yjeM"
                     /locus_tag="ACHJ7J_003604"
     CDS             9427..10929
                     /gene="yjeM"
                     /locus_tag="ACHJ7J_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5001788.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11080..14403)
                     /gene="mscM"
                     /locus_tag="ACHJ7J_003605"
     CDS             complement(11080..14403)
                     /gene="mscM"
                     /locus_tag="ACHJ7J_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5001789.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LVILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14425..15393)
                     /gene="asd"
                     /locus_tag="ACHJ7J_003606"
     CDS             complement(14425..15393)
                     /gene="asd"
                     /locus_tag="ACHJ7J_003606"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5001790.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15490..16542)
                     /gene="rsgA"
                     /locus_tag="ACHJ7J_003607"
     CDS             complement(15490..16542)
                     /gene="rsgA"
                     /locus_tag="ACHJ7J_003607"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5001791.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16637..17182
                     /gene="orn"
                     /locus_tag="ACHJ7J_003608"
     CDS             16637..17182
                     /gene="orn"
                     /locus_tag="ACHJ7J_003608"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5001792.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17393..17468
                     /locus_tag="ACHJ7J_003609"
     tRNA            17393..17468
                     /locus_tag="ACHJ7J_003609"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17426..17428,aa:Gly,seq:gcc)
BASE COUNT         4015 a         4526 c         4603 g         4344 t
ORIGIN      
        1 tatgaataat taaaggttat gcaacgggca aagattaaac ttccctgttt aatgacaggt
       61 tgctgtactt gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca
      121 gttcattgca aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc
      181 aggcaatttt aatgtttgac ttccctgttt aatcagttac tgctggcgta gctggagatc
      241 cagcggcgtt ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc
      301 cgacaccagt gtcagcaact cacgcataat ctgccgcgct tcgtcatcac tcaccataaa
      361 atgcgccgcg ccctgtactt tatcgagcac atgcaggtaa tacggcatta cgccggcatc
      421 gaacaacgca ttactcaggt ttgccagcgt ttgtgcgtta tcgttcacat cacgtaacag
      481 aacgctctgg ttcagcaaag taacgcctac ccggcgcaac ttagccatcg cctgacggaa
      541 tgtttcatct acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga
      601 acgggcaaag cgttcaacca gcgcatctgt gatgcgtgcc gggatcacaa tcggcagacg
      661 actgtgaatc cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc
      721 cagctcgtga tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc
      781 cggatgcgca gcaacatact caagtgcagt ttgccagtta cgcttgttgc cctgattttc
      841 tgcataaggg aagtggcgac ggaagcaata acggcaattt accgcgcagc cgcctttgac
      901 cagcaaaagc gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc
      961 ttccagcggg tcggtggaga atccgggcgc gatggcaaac tcatcttgcg aggtaattac
     1021 ctgacgcaaa agcggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg
     1081 cacacgcagg gcaaacagct ttttggcgtt gcgtccggcc aacagttttt cgtccgcgtc
     1141 tatattcaaa agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa
     1201 ccaatcttct ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta
     1261 ccaattaaca aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg
     1321 gtcttaaaat catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac
     1381 cgggtaaagg ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg
     1441 tagaaaaaac cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc
     1501 tgacttacct gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc
     1561 agctgtctgc tgacgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag
     1621 agtgtatcgt aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg
     1681 aactggaaat cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca
     1741 aaccggctac cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg
     1801 aagtcatcaa agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt
     1861 gtggtgcggc ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta
     1921 tcgttcttgt tttgcttgcc agcacgttgc tcacgggctg taacaccgct cgcggtttcg
     1981 gcgaagacat caaacatctc ggcaactcca tctcccgcgc tgccagctaa tttttcttct
     2041 cttccgaaaa atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct
     2101 ataggcaaac ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc
     2161 agcgatcttt tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg
     2221 cgttggtgaa gacatttctg atggcggtaa cgcgatttct ggtgcagcaa cgaaagcgca
     2281 gcaataagca ataacggtac gacagctgtg tcgtgccgtt tgttttttct gcgatagtca
     2341 ctaaggtaat agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt
     2401 tgcaggacga cctgcaaacg cctcttttca ccggggacgg ccccaattct ccggagcctg
     2461 atatgtcctg gattatctta gttattgctg gtctgctcga agtggtatgg gccgttggcc
     2521 tgaaatatac ccacggcttt agtcgtttga cgccaagtgt tattaccgtg acggcgatga
     2581 ttgtcagtat ggcgctactt gcctgggcga tgaagtcatt acctgtaggg acggcttatg
     2641 ccgtgtggac gggtattggc gcagtcggcg cggccatcac cggcattgtg ctgctcggtg
     2701 agtccgctaa cccgatgcgc ctggcgagtc tggcgttaat tgtattgggg attattggtc
     2761 tgaaactcag cactcattaa ctaccgggct gctgtaccca aataaactta ctgacatcaa
     2821 acccttcccg ggtcgcgact gccagcatct cctgtttcac ttcgtcagaa atggttggcg
     2881 tgcgggagag tatccacagg tagtcgcggt ccgggccgca aaccagcgca tggcggtatt
     2941 cccgatcgag tgcaataacg ttataaccgc catagaaagg accaaagaat gacactttca
     3001 gcgcagcgcg agttggtgcg ccggtaaagt acgctttccc ttcactctgc tgccacattt
     3061 ctctgtcagg gttatagcct ttattaatga cattcaggcc gccatcatcc ctcaggctgt
     3121 atgttgcggt gactttttcc agtccacgtt caaagcggtg atcaaaacgg gcaatctcat
     3181 accaggtacc gagatagcgt ttagcgtcga aattattcac tacggtcacg ccacgcggcg
     3241 gcgtaggaga actgcaggca acgaccagaa atgcagctgt cgctgcggca acgagaggga
     3301 gcaggcgcat aaatgtttcc ttactggttt ttttctaagt gtagatgaca gcaaggaaaa
     3361 gcggagaaaa ggtccgaaaa ttcggacccg atgaaatttt actgcagagc gttgagaatc
     3421 tgccaggcgg cggcgactct cgctggattg ggatagtttt tgttagccag catcacgata
     3481 cccagctctt tttctggaat aaacgcgaca tagctaccaa atccgccggt cgcccccgtt
     3541 ttatgtaccc atgatgcgcg tactgcagga gttgggggcg taatcgcttt tacggggcgt
     3601 gctgccagtg caattttatt gtcactgccg ttaacgatga tgtcaggatt taccggccag
     3661 tccagcattt cccagcccag accctgatac atatcgccgg tttgccagta gcgagattgt
     3721 gccagttgta tcccttgttg aagcgttttc tcattgatat caaggggttt taaattgatt
     3781 tgcacccagc gggccatatc ttcaatggtc gacttcacac cataagcttc agcatctaac
     3841 gcccctggcg aaacatgcac tgccttacct tcgcgatatc cccaggcgta attcttttct
     3901 tctgcgggcg gtacattaat ccacgtatgg ttgagtttga gtggctggaa gacacgagtt
     3961 tgcatcgcct gctcaaaact caaaccagac ggcttcacag ccagtgcgcc gaacaaaccg
     4021 atactggagt tggcatacag acgttgtgtt cctggcgccc atgcaggctg ccagttttga
     4081 tagaagcgca gcaagtcgct tgaggatttc acctcatccg gcacctgcaa tggcaggccg
     4141 ccagcggtgt aggttgcgag atgtaatagc gtgatcccgt tccactgttt agcggtaagt
     4201 tcaggccagt attttgttgc gggatcgctt aacttgattt cccctcgagc aatagcgtcg
     4261 ccaccaagca cgcccgtaaa tgttttgctg accgaaccta actcaaacaa cgtttgctgt
     4321 gtgacgggct gctttttggc gatgtccgca tagccccagg taaagtaata aggtttaccc
     4381 tgataaatta ccgccaccgc catacccggg atcttttgtt gctctataag cggggtaatt
     4441 gtgcgatgca caatatcgtt gatttgttga ggggcagcaa atgtggagca agaggcggta
     4501 attaataagg cgcagagcgt cgttttgaac atagggtctg gtttccatac aaaacggccc
     4561 gccataggcg ggccggattt acattggcga tgcgttagat tgtaacgaca ccaatcagcg
     4621 tgacaactgt caggatagca gccagaccgt agaaaaccca tttgcccgca ggtacgtgga
     4681 ttttcagatc gtgcatcgcg tggtgcatac ggtgtaaacc acaccacagc ggcagaacga
     4741 tcatcaggaa caggaatacg cgaccaatga agctctgcgc gaacgccaga acgcgctcgt
     4801 agctcagtgc atcacccgga aacagcccca gtggcagcag aatacccacc agaaggatca
     4861 tcaccggcgc aatgatggcg ctccacatac caccggcccc gaagaggccc cagaataccg
     4921 gttcgtcaga acgctttgga tttggattaa tcatctcagg ctccttacca gtacagggca
     4981 acaaacagga ttacgatggt ggcaaccaca gttaccgccc agagactttt gataattggc
     5041 tctggtccca ttttttcgtc ttttacaatg atattggccg ctttcggtgc cagttcaaac
     5101 caggttttgg tgtgcagcag agctgccgcc agagtgatca ggttaatgat cacaataacc
     5161 gggttttgta aaaagtcgac gaatcccgcc caggcttccg ggccattttt cagggcaaac
     5221 agcccgaaaa tcagttcaat gctgaaccac acagccggaa ccgccgtgcc ttcgcgcagc
     5281 atgtaaaagc gataaaacgg caattttttc caccaggtgg acgtcattgg ccgtacatac
     5341 ggtttacgtt tagtcgtcat gttgcactcc ttagcgtggt ttcagggtcg cgataagaaa
     5401 gtctttcgaa ctttctactt tgccctgctg aatggccgca gccggatcga cgtgtttcgg
     5461 gcagacttcg gagcagtagc ccacgaaagt acagctccat acgccgttct ggctgttcaa
     5521 ctgcgccata cgctccttct taccgtggtc gcggctatct tcgttataac gatgcgccag
     5581 cgtaatggca gccggaccga tgaactctgg gttcaggcca aactgcgggc acgcggcgta
     5641 gcacagacca cagttgatgc aaccggagaa ctggtgatac ttggccatct gcgccggggt
     5701 ctggatgtta gtaccctgat ccgcggtgcg ggagttgccg atgatgtacg gtttgatcgc
     5761 ttccagactt tcgatgaagt gggtcatatc gaccaccaga tcgcgttcaa tcgggaagtt
     5821 agctaacgct tcaaccttca taccgtcggt gtaatcacgc aggaaggttt tacatgccag
     5881 ttttggcacg ttgttaacca tcatgccgca ggaaccacaa atcgccatac ggcaggacca
     5941 gcggtagctc aggtccggtg ccaggttgtc tttgatgtag cccagcgcat ccagtaatga
     6001 ggtagttgcg tcataaggca cttcatagaa tgcgctatgc ggtgcggtat cgacttccgg
     6061 gttatagcgc accacctcaa ttttcaggtt tttcatctca gccattcgcc ttctccttct
     6121 tattggctgc ttccgcctta tcggctgcat ccgcttcgcc accgtaaacg cgtttagctg
     6181 gcggcagcgt agtaatcttc acgtcgctgt actccaggcg agtcgtgcca tcagcatcgc
     6241 ggaaggcgag ggtgtgtttg aggaagttga cgtcgtcacg ctcggtgcaa ccttcgtcca
     6301 gacgctggtg tgcgccgcgg gactctttac gtgccattgc ggagtgcgcc atacattcag
     6361 caacgttcag accgtggccc agttcaatgg tgtagagcag gtcggtgttg aacacgctgg
     6421 aagtgtcggt gatgcgcacg cgcttgaagc gttcctgcag ctctgccagc ttgtcgatgg
     6481 ttttctgcat cagttccggc gtacggtaga taccgcagcc ttcttccata gccaggccca
     6541 tttcgtcgcg gatcttcgcc cagttttcgc cgccatcctg gttaaccaga tctttcagac
     6601 gttgttcaac gccagctgcc tgcgcttcaa ttgccgcttc gttgccatta ccggcagttg
     6661 ctgcacgctc tgtcgcttgt tcaccggcca gacggccgaa gaccaccagt tccgccaggg
     6721 agttagaacc cagacggttt gcaccgtgca gaccaacaga ggaacattca cccacggcga
     6781 acagaccttt aatgcgggtt tcacagttct gatcggtttc gataccgccc atggtgtagt
     6841 gtgcggtcgg acgtaccgga atcggttctt taaccggatc gacgccaacg tacgctttcg
     6901 ccagttcgca gatgaacggc agacgttcat gcagtttttt ctcgccgagg tgacgcaggt
     6961 cgagataaac cacatcgcca cgcggcgtgg agatggtgtt gcctttacgc cattcgtgcc
     7021 agaaggcctg agaaactttg tcgcgtggac ccagttccat atatttgttt ttcggctcgc
     7081 ccagcggagt ttccgggccc atgccgtaat cttgcagata acggtagcca tttttgttga
     7141 ccagaatacc accttcaccg cggcagcctt cggtcatcag gataccggaa cctggcagac
     7201 cggttgggtg atactgaacg aattccatgt cacgcagcgg aacgccgtgg cttagcgcca
     7261 tacccatacc gtcaccggta acgatgccgc cgttggtgtt gtaacgataa acgcgacccg
     7321 caccgccggt agccataacg accgcgttag cacggatctg caccagcgtg ccttccatca
     7381 tgttcattgc taccaggccg cgaacatgac catcatcaac cagaatatcc agtacgaaat
     7441 gttcgtcaaa acgctggatc tgcgggaatt gcagagaggt ctggaacagc gtgtgcagca
     7501 tatggaagcc ggtcttatcg gcggcgaacc aggtacgctc gattttcatg ccgccgaagc
     7561 gacgtacgtt gacgctacca tccgggcgac ggctccatgg gcacccccac agttccagtt
     7621 gggtcatttc ggttgggcag tggtggacga aataatccac gacatcctgc tcacacaacc
     7681 agtcgccacc cgctactgta tcgtgaaagt gatattcgaa gctgtcatga tcctgcgcga
     7741 cagcggcgga gcccccttct gcagcaacgg tatggctacg catcgggtat acttttgaga
     7801 ttagtgcgat ttttgcattt ggatttgcct gcgcggcagc aattgcagca cgtaatcccg
     7861 cgccaccggc gcctacaatg gcaagatcgg cttgaaaggt ttgcacgaca ttcctccaga
     7921 ttgtttttat cccacagccc cgtacttcag ggtaagtgcc tgaaagttac ggtctgcgaa
     7981 cgctattcca ctgctccttt ataggtacaa cagtatagtc tgatggataa gtctgaaatt
     8041 tgacgagatc gattttttta gtgcgcaaga gggctaaatt atcactgaag atgattaatt
     8101 taattactaa accatcagat cgtgcttttt ttagtcactg cccaccgttg ttttattcct
     8161 gcgtaaaatt tgtttcgccg tcgcgttgcg agtagacttc gtgcccttgt caaaaactgg
     8221 agatttaact atgagcgaaa cggcatcctg gcagccgagc gcatccattc ctaacttatt
     8281 aaaacgcgcg gcgattatgg cggagatccg tcgtttcttt gccgatcgtg gagtgctgga
     8341 ggtggagacg ccttgtatga gccaggcgac ggtaaccgat attcatttgg tcccgtttga
     8401 gacacgtttc gttggccccg ggcattcgca ggggatgaat ctctggttaa tgaccagccc
     8461 ggaataccat atgaaacgcc tgctggttgc cggttgcggg ccggtattcc agctgtgccg
     8521 cagtttccgt aatgaagaga tggggcgtta tcacaaccct gagttcacta tgctggagtg
     8581 gtatcgaccg cactatgata tgtaccggtt gatgaacgag gtggacgacc tcttacaaca
     8641 ggtgctggac tgcccggcag cagaaagcct ttcttatcaa caagctttct tgcgttatct
     8701 ggaaattgac ccgctctctg ccgacaaaac gcaactgcgg gaagtcgcag cgaaactgga
     8761 tttgagcaac gttgctgata ccgaagaaga ccgcgacacg ttgctacaat tgctgtttac
     8821 ctttggcgta gagccaaata ttggtaaaga aaaaccgacc tttgtgtacc actttccagc
     8881 cagccaggca tcactggcgc aaatcagtac cgaagatcat cgggtcgctg aacgctttga
     8941 ggtttattat aaaggtattg agctggcgaa tggtttccat gaattgacgg atgcccgtga
     9001 gcagcaacaa cgctttgaac aagataaccg taagcgcgcg gcgcgtggtt tgccgcagca
     9061 ccccattgac cagaatctga ttgaagcctt gaaagtcggt atgcctgact gttccggcgt
     9121 ggcattgggc gttgatcgtc tggtgatgtt ggcgctgggt gcggagacac tggctgaagt
     9181 catcgccttt agcgttgacc gggcataatt ctgaaaaatt actgaattaa cagcgaagaa
     9241 tggcgtgata agcgccattc tgtagcatat attttcattt ttccgctaaa ggccacaatt
     9301 taagactgga tatttgatat catccaggta tcaattctgt attggttttt acgctgcccg
     9361 ctcaatgttg ggcagtgcag ttcagtgggc gcgttttata cacgcgctga aatgaaggat
     9421 ggtttcatgc ctcacacgat aaaaaagatg agtctgatag gactcatatt gatgatcttt
     9481 acttccgtat ttggatttgc caatagccca tcggcttatt acttaatggg ttatagtgcg
     9541 attccctttt atatattttc tgcattgtta ttctttattc cattcgcctt aatgatggct
     9601 gaaatgggag cggcttatcg caaagaagaa ggcggtatct attcctggat gaataatagt
     9661 gtcggaccac gttttgcctt cattggtacg tttatgtggt tttcctctta tatcatctgg
     9721 atggtgagca cctccgcgaa agtttgggta ccgttctcaa cattcctcta tggtagcgac
     9781 atgacccagc actggcgtat tgccggactg gagcctacgc aggtggttgg tctgctggca
     9841 gtggcatgga tgattctggt caccgtcgtt gcttctaagg ggattaataa aattgcccgc
     9901 attactgcgg tgggcggtat tgcagtaatg tgtctgaatt tagtattgct gttagtaagc
     9961 attactattt tgttattaaa tggtgggcat ttcgcgcagg atattaattt ccttgcatca
    10021 ccgaacccgg gttatcagtc cggtctggca atgctatcgt ttgtggtatt tgctattttt
    10081 gcctatggcg gaattgaagc ggttggtggt ctggtcgata aaacggaaaa tccagaaaag
    10141 aactttgcca aaggtattgt ttttgccgct attgttattt caatcggtta ttcgctggca
    10201 atatttttat ggggcgtcag cacaaactgg cagcaggtat taagtaatgg ttccgttaac
    10261 cttggcaata ttacctatgt gctgatgaag agcctcgggg tgacgctggg taacgcactg
    10321 catttgtcac ctgaagcgtc attgtcgctg ggcgtatggt ttgcgcgtat taccggactt
    10381 tcgatgttcc tcgcttatac cggtgcgttc tttacgcttt gctattcacc gctgaaagcc
    10441 atcatccagg ggacgccgaa agcgttgtgg ccggaaccga tgacgcgcct gaatgcgatg
    10501 gggatgccgt ctatcgccat gtggatgcag tgcgggttgg ttactgtctt catcctgctg
    10561 gtttcgtttg gtggcggtac cgcatcggcg ttctttaaca agctgacgct gatggcgaac
    10621 gtgtctatga cgcttcctta cctgttcctc gcgctggctt tcccattctt taaagcacgt
    10681 caggatctcg acagaccatt tgtgattttc aaaacgcgta tgtcggcaat gattgcgacg
    10741 gtggttgtcg ttctggtggt gacatttgcg aacgtcttca ccattattca gcctgtggtt
    10801 gaagccggag actgggacag cacattgtgg atgattggcg gccctgtctt cttctcgctg
    10861 ttagcgatgg cgatttacca gaactattgc agtcgcatgg cgaataaacc tgagttagct
    10921 ctcgactgat catatgaaat cctgcaactc atcgtctctc cggcgatgag ttgtcaggaa
    10981 gtaatgcgta tggatgatac tctccagggc cggtgataac tgaagatgat gttaagctgc
    11041 taaatgagcc acgttgacat catttggcag cttcatgcat tacaaacttc ccgcctgacg
    11101 acctttgccc gcagaagtta atgttctccc cgtttgttta ccgttgaggc tttccagacg
    11161 catctggaag ggcgggaatg gcatatcgat accgtgggca tggaagccag ccagaatcag
    11221 ctggtggatc tcatggcgta gcggcatacg gtgacccatc tcagcggcgt aaatacgcag
    11281 ctcgaaaatc tgaatcccct gttgcagatc caccaggaag acttccggtg ccgggttgtc
    11341 gatgaccagc gagcagcgac gcgctgcggt gagcaggatt tccgtcactt cttcactgtt
    11401 ggcatcggca ggggccggaa tcgtcaacac cacgcgcgtg accgagtcag agagcgacca
    11461 gttgataaac tgctcggtaa taaacgcctt gttcggcacg attatctctt tacggtccca
    11521 gtcgctgatg gtggtggcgc gggtgttaat ttttgtcacg ctaccagtaa gatcgcggat
    11581 cgttaccgta tcgccaatgc gaatcggttt ttcgaacagg atgatcaggc cagagataaa
    11641 gttggcgaaa atttcctgca aaccaaaacc gagaccaaca ccgagcgcgg caaccagcca
    11701 ctgcaatttc gaccactcaa taccaatcat tgagaagccg accagcccgc caatcagcat
    11761 cagcagatat ttggtgatgg tggtgatggc gtaacccgta cccggcgtta aatccaggtg
    11821 ctgcaaaatc accagttcca gcagcgcggg caggttgcgc accagttgcg tggtgatgat
    11881 aaacaccaga atggcaatca gcaccgcacc gagggtaatt ggctccagac tttctacgcc
    11941 ctgtaccgtg gaggtgacat cccacagcga aatattttcg aggaagccga aagcggaatg
    12001 gatttctgac cacagcacaa tgaccgaaag cagggcgatc aacatcaaaa ttgagcgcac
    12061 cagccgcaag gattgcgcac tgatggcatc gagatcgact tcgctttcat cgacttcaat
    12121 tgccccttcc gggctactgt gatgatgcgc ttcctcttcg ccacgcgcgc gttgcgctaa
    12181 catctctgcc cggcgatgct tcgcccggtc aaacgccagc ctgcggcgct ggatcagcat
    12241 ccagcggcgg ataacgtgat aaaccaccag tagcaggaac cagatggcaa ccgaggtttc
    12301 aagcctcgct aacagtgcct gcgccgttgc cagataaccc accgccgacg ccagaatggc
    12361 aaccagtggc gcgccaatca tcatgttcca cagcatatgg ttggtaatgt tgtcgccgct
    12421 gccctctttg ttgagataca gcggtatccc ggcctttttc aggctgaggg tgaccaccgc
    12481 cagcgcaccg caaatgagga taaagcaaag ccgtcccagc gaaccggaaa attcgcggtc
    12541 gtcgaggtta tcgaacatca tcagcgccat aatcagtggc acaataagcc cgatgctcat
    12601 caggtagtag cgcatcccac gagaaacacg ttcgcgcggc cagccaaaat gagcgataaa
    12661 caagccgttc gggcgggcaa aagtagcgca aatcatcact acccacagca gcggcacggt
    12721 ggctgttacg ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc
    12781 gtaacccagc gtcatccaca gcaccggcag cggtgacgcg acgagaatcg accagaaaag
    12841 agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg
    12901 ttcgagaaaa cgggtaaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc
    12961 gccaaacagc ggcaaaatcg tctctttgct ggtcagcatc atcacactgg ctttgcccaa
    13021 ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca
    13081 ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtgcg tcgcttcgtt
    13141 cacctctttc agcgcatcct ccagttgccc gttagagact ttcagcttgg tcagttccag
    13201 cagtagtgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgtagctg
    13261 tgcttcgaga atacggtttt gctcggcagt cagcggctga ccgtcggcct gatgaatttg
    13321 ccgtagcagc ggctgtttat taagcagatc ctcataacgt aaccgttgca cacgcaactg
    13381 cgccatttcg gtatcaagct gttgtggttt cggcatttcc ggcagccgtg ccacctgcgc
    13441 ccgcaacgct tcaccgagca gattggacga tcccagccat tgcgactgtt cacgcagcgt
    13501 attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac
    13561 gagatccatc cgctgcgcct gttgattcaa agccgccgat agttcgcggt taattttgaa
    13621 ttgcgcgacg atatctttcg gcaaatcggc actgttttct gccagcaatt cggtactttc
    13681 cagcgcccgc tctgcctcaa gctggcgttg gctgtttaat tgattacgta aggcctgcaa
    13741 atacgcatcc agttgctggc tctctttttc agccagctca gagcgcaagc gcgctaattc
    13801 ctggcggtta ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg
    13861 tgcagagtca gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag
    13921 cgttcccagg cggcgctcga tctcgtttaa ctgacggcgg gcgtcggttt gctgttgcgg
    13981 cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg
    14041 gcttttatcc agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga
    14101 catccccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg cgcgcagagt
    14161 agcggagagt ttcggataat tatcaatgac ttcttgatat tgcttgatgc gctcaaggga
    14221 accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg
    14281 tgcgggtttc gccgcttttg cctgctccag ttcttgtgag atttgtttgc tatcgggggc
    14341 cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg
    14401 cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct
    14461 ggcgtcgtgt tctgcttcga tctcttcagc aggaagcggg gcgggttcag cgtctggcgt
    14521 aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc
    14581 cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag
    14641 tttaaagcga cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc
    14701 aggccaggtc cagcgcttga tgatgccttc gcgcggcggc gtaatggtgc ccgcccagac
    14761 agtctcaatg ctgcctacaa tcgtcgcacc gaccagaatc tgcgccattg ggccaaattc
    14821 ggtatcgaac aggcagatca cgcgttcgtt acgggcaaac agattcggca cgttctgagc
    14881 cgtgagatgg ttaacggaga agagatcgcc cggcacgtag atcatctcac gaagaatacc
    14941 gttgcacggc atgtgtacgc ggtggtagtc acgcggggag aggtaagtgg tcacaaacgt
    15001 accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta
    15061 gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac
    15121 gccatcggca ggcataacca ggacattcgg atcggtatcg attgggcgta cttcgtcacg
    15181 cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc
    15241 ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca
    15301 tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat
    15361 gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg
    15421 ttcctgatcc ggcctacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta
    15481 gcttaattgt cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct
    15541 ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc
    15601 ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc
    15661 taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag
    15721 gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag
    15781 ccgagcggcg gtcgtggtgt gctgaccaag ccccgagttg tcagagacat cgttggtcag
    15841 gatctctttt tgcagcccca gcaacgcgtt cagcaggctg gatttgccga cgccagactg
    15901 cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttta gcccatcctg
    15961 agtatggctg gaaaccatca atacgcgata accgatattg cggtagatat ccatctgttc
    16021 gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca caataatcgg
    16081 ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga gtgacagctc
    16141 cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggcaatag gtttcacgcc
    16201 gtcgtagaaa tccggacgcg tcaacaccga ggtgcgctca tgcaccgctt ccacgatccc
    16261 tttgacattt acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt
    16321 caccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc
    16381 cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag
    16441 attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt
    16501 cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtacag actcctggtc
    16561 gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga
    16621 atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat
    16681 gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc
    16741 caatctgaat attctggcag aagggccgac cattgcagta caccagtccg atgaacagct
    16801 ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt
    16861 gaaagcgagc acgatgggcg atcgtgaagc tgaactggca acgctcgaat tcttaaaaca
    16921 gtgggtgcct gcgggaaaat cgccgatttg cggtaacagc atcggccagg atcgtcgctt
    16981 cctgtttaaa tacatgccgg agctggaagc ctacttccac taccgttatc tcgatgtcag
    17041 caccctgaaa gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca
    17101 ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg
    17161 cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgtcgga atccacttgc
    17221 ggcggcgtga ttttgacgct aaattgaacg ccttgctgat aatttcagcg cttgagataa
    17281 aaactcaaaa aaaatttttt aggggggttg cagagggaaa gatttctcgt ataatgcgcc
    17341 tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat
    17401 agctcagttg gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc
    17461 ccgctccaaa atttgaaaag tgctgcaa
//
LOCUS       DAZFFG010000094        17374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFFG010000094 DAZFFG010000000
VERSION     DAZFFG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.094"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            269..1612
                     /locus_tag="ACHJ7J_003610"
     CDS             269..1612
                     /locus_tag="ACHJ7J_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001793.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1761..3269)
                     /gene="putP"
                     /locus_tag="ACHJ7J_003611"
     CDS             complement(1761..3269)
                     /gene="putP"
                     /locus_tag="ACHJ7J_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO5001794.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            3691..7653
                     /gene="putA"
                     /locus_tag="ACHJ7J_003612"
     CDS             3691..7653
                     /gene="putA"
                     /locus_tag="ACHJ7J_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5001795.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTMLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVAEGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGCLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENNEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7693..8331)
                     /gene="rutR"
                     /locus_tag="ACHJ7J_003613"
     CDS             complement(7693..8331)
                     /gene="rutR"
                     /locus_tag="ACHJ7J_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO5001796.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8619..9710
                     /gene="rutA"
                     /locus_tag="ACHJ7J_003614"
     CDS             8619..9710
                     /gene="rutA"
                     /locus_tag="ACHJ7J_003614"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO5001797.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKSIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQTQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9710..10402
                     /gene="rutB"
                     /locus_tag="ACHJ7J_003615"
     CDS             9710..10402
                     /gene="rutB"
                     /locus_tag="ACHJ7J_003615"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO5001798.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10414..10800
                     /gene="rutC"
                     /locus_tag="ACHJ7J_003616"
     CDS             10414..10800
                     /gene="rutC"
                     /locus_tag="ACHJ7J_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO5001799.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10808..11608
                     /gene="rutD"
                     /locus_tag="ACHJ7J_003617"
     CDS             10808..11608
                     /gene="rutD"
                     /locus_tag="ACHJ7J_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO5001800.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11618..12208
                     /gene="rutE"
                     /locus_tag="ACHJ7J_003618"
     CDS             11618..12208
                     /gene="rutE"
                     /locus_tag="ACHJ7J_003618"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO5001801.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDRSKLFARLPRLSFEEACGLL"
     gene            12219..12713
                     /gene="rutF"
                     /locus_tag="ACHJ7J_003619"
     CDS             12219..12713
                     /gene="rutF"
                     /locus_tag="ACHJ7J_003619"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO5001802.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSIGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12734..14062
                     /gene="rutG"
                     /locus_tag="ACHJ7J_003620"
     CDS             12734..14062
                     /gene="rutG"
                     /locus_tag="ACHJ7J_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO5001803.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVVKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14145..14318)
                     /gene="ymdF"
                     /locus_tag="ACHJ7J_003621"
     CDS             complement(14145..14318)
                     /gene="ymdF"
                     /locus_tag="ACHJ7J_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5001804.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            14691..15287
                     /gene="wrbA"
                     /locus_tag="ACHJ7J_003622"
     CDS             14691..15287
                     /gene="wrbA"
                     /locus_tag="ACHJ7J_003622"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5001805.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15308..15535
                     /gene="yccJ"
                     /locus_tag="ACHJ7J_003623"
     CDS             15308..15535
                     /gene="yccJ"
                     /locus_tag="ACHJ7J_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO5001806.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15573..16814)
                     /gene="agp"
                     /locus_tag="ACHJ7J_003624"
     CDS             complement(15573..16814)
                     /gene="agp"
                     /locus_tag="ACHJ7J_003624"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO5001807.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(17096..>17374)
                     /locus_tag="ACHJ7J_003625"
     CDS             complement(17096..>17374)
                     /locus_tag="ACHJ7J_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001808.1"
                     /translation="DTDTSDRIKAVKNFLSVLSDSICSLFIVNSNVASISLGNESFST
                     DEDLEYGYLINTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI"
BASE COUNT         4138 a         4581 c         4569 g         4086 t
ORIGIN      
        1 ttatttaccg cgatgataca aggcgtaaaa aaaataataa tcttcatgaa actcaccccg
       61 atggcagcat tgtaaattcg ccaataactg gcattcctcc tgctttttac gggtgtttcc
      121 attaacacca gaatgagcgg attgtttact acatcaagaa cgcatccgaa gtgtacatcc
      181 tggtgcgccg actgaatcgc ccggagccat gcggatggct acgacttaat acgaatcatt
      241 aatcctgtaa ggatacggag agcatgacat gagaccaata tccaacctca atacgagcgc
      301 agcaacctat attccaccgc aacaactccc gagtagttgt ctggaaactt tgacgcttct
      361 ggtgcagatg aataattata tcaaaaggga tgcggactac agcacgggca tggctgttac
      421 tccactggtt cctgaagagg ttcacttgct ggctgttgcg atgacaaccg aactacatcg
      481 acatcagttc caaccggtgg taagtgcaaa tgatctgcaa cttcctgaac cgttcacctt
      541 cgatgttgcc ggtttcacca tcaccttcac gaagacgcaa gagcatgaca acacgggcaa
      601 tctcatgaaa attgcagtca gcaagagcgg tgtgtgcacc agtacaaata ttactcttga
      661 actgtttcac tccattgtca caaccctgat gtcacgcagt caatatggaa cctttgatct
      721 ctggtccgta cggccaatac tcacggatga aagtcaaaac agggtgcatg aagcagctcg
      781 ctactccccg gcccagcaat acggaagaga agaaacacac tttaccaaca atggcatgcg
      841 agaatttgga aatatgtctc cgctggcgtg gcgtaacgat gtggaattac agcaatcgtg
      901 taatacaagc aacatcccac caaatgctgc aaatgacgac ataaacaaca ccaggcagac
      961 catcgaggac cagccagagg cggacgagcc acagcaatat gttaaattga ctgtagacga
     1021 catgcgaaaa tgggcggcta tggaccagca ggcgcgaaac gctctacagg gagtttcagg
     1081 atggtgtacg cgcaatcatt ttaatatcaa aaatgccaga aattacctga ccgatcacgg
     1141 cctgaactac gcgggacagg taaaggttaa caggccgcat gaatatgcca aattcaccct
     1201 tgaacatatc cgccagtggg cagccttaaa caagcatgtg cgaaaaccgg ttggttattt
     1261 agaaaagtgg tgtaaggaaa gaaacctcgc gccaactact gccaggaatt acctgaagaa
     1321 cgacgggctt accgccctgg gtgagttgaa gctgacaggg ccgcagaaat gggtcacgtt
     1381 caccttcgga gacatcgtac aatgggcgaa tatgacccag gaggagcgta acagcgcagg
     1441 gggagccgaa aagtggagta aaaaacgcgg cttccagtgg tctactgcca gatcctacct
     1501 gaaatcgagc ggagtgacca agcaaggtgc gagaaaactg gcgtggttaa aaaacagcgg
     1561 gaatatgtcg aatccattct atctcgccca accaaccagc cgcaggacat aagctgaagg
     1621 taccgatagc ggcaattttg tttatctgtg ccgggtgggc aaatagcaat tgcccccgaa
     1681 cactccctgc caggcaactt ataccgcatg gcagggaaca ttcgggttaa aaagcaaaag
     1741 ataaataatt actcttcagc ttagccttcc tgcaaccgtg acggcggtgc cgaatgatag
     1801 tgcgcatcgg cctcggcaaa gcgtttttgc atcgccgctg acggcgcttt acccagcaaa
     1861 ctaaacacta caatcccaat actgccgaag ataaagcccg gaataatttc gtacagtccc
     1921 agccagccga actgtttcca gacgataacc gtcagcgccc cgatgatcat ccccgccagc
     1981 gcaccgttgc gcgtcatgcg tgaccacatc accgagaaca gcaccaccgg accaaacgcc
     2041 gcgccaaagc ctgcccacgc gtagctcact aagcccagca cgcggttttc cgggtttgcc
     2101 gccagcgcaa tggccaccag cgccaccacc agcaccatca cacgccctac ccacaccagc
     2161 tctttctggc tggcatgttt acgcagaaac gctttgtata aatcttcggt aatcgcactg
     2221 gagcacacca gcagctggca acttaaggtt gacattaccg ccgccagaat tgccgacagc
     2281 agaatcccgg caatccacgg gttaaacaga atttgcgcca gttcgataaa cacgcgttca
     2341 gcgttctggt ttaccgcacc tgctaccgcc gggtgctcgt taaagtaagc gataccaaag
     2401 aagccgacag ccaccgcccc tgccaggcag aggatcatcc aggtcatact gatacgacgc
     2461 gcatggacga tgctgtggtg agaatccgcc gccataaaac gcgccaggat gtgcggttgc
     2521 ccgaagtaac ccaggcccca ccccatcagc gagataatgg cgacaaagtt cagccctttg
     2581 agcatatcca cgttttcgat gctcttttgt ttgatcactt ccagcgagtc accaaagcca
     2641 ccgacactga taatgacgat aaccggcgtc aggatcaggg caaaaatcat caggctggcc
     2701 tgtacagtgt cagtccagct caccgcgagg aaaccgccaa taaaggtgta aaggatcgtc
     2761 gccgcagctc ccgcccacag agccgtttcg tagctcatgc caaaggtact ttcaaacaga
     2821 cgcgcgcctg ccacaatgcc cgaagcgcaa taaatggtga agaacagcaa aataactagc
     2881 gcggagataa tgcgcaaaat gcggctttta tcttcaaaac gcccggtgaa ataatccggc
     2941 aatgttaagg cgttattgtt gtattcggta tgcacacgca accgcccggc caccagcttc
     3001 cagttaatcc acgcgcctaa tgtcaggcca atggcgatcc aactttcgga aatcccggaa
     3061 agaaaaaccg cgcccggcaa ccccattaac agccagccgc tcatatccga tgcacccgcc
     3121 gataatgccg tcacgaatgg cccaaggcta cgtccgccca gaatatagtc gtcaaagttt
     3181 ttcgttgatc gccaggcgat aaacccaatc aatatcatgc caaagatata gacacaaaat
     3241 gtcaccaaca tcggtgtgct aatagccatc taaagtctcc aaaaaattat tatcggcaat
     3301 gtcgaaactt gccgttatat ctgccaccgg aacggggtaa cagagtttat gttttaccag
     3361 ggcgaccgta tcctgccgga agcgctagtt actcacaatc gatttaacac accatttaca
     3421 ttaaatttta gtgctcagcg acactatttt tcatcaggtt gcactctctc acattttttg
     3481 cggttgcacc tttcaaaaag ttaactgccg cagagaaaaa gtctgagtta tttttttaat
     3541 ccctgtcata tcgatttctt ttattaacat ttcattcatt tttaagcttg ctacgcaggt
     3601 cacatttaac atggttgcac aaagttgcaa catcatggat atttcacgat aacgttaagt
     3661 tgcacctttc agaacaacag gagtaatggc atgggaacca ccaccatggg ggttaagctg
     3721 gacgacgcga cgcgtgagcg tattaagtct gccgcgacac gtatcgatcg cacgccacac
     3781 tggttaatta agcaggcgat tttttcttat ctcgaacaac tggaaaacag cgatactctg
     3841 ccggagctac ctgcgctgct ttctggcgct gccaatgaga gcgatgaagc accgactccg
     3901 gcagaggaac cacaccagcc attcctcgac tttgccgagc aaattttgcc ccagtcggtt
     3961 tcccgcgccg cgatcaccgc ggcctatcgc cgcccggaaa ccgaagcggt ttctatgctg
     4021 ctggaacaag cccgcctgcc gcaaccagtt gctgaacagg cacacaaact ggcgtatcaa
     4081 ctggccgata aactgcgtaa tcaaaaaaat gccagtggcc gcgcaggtat ggtccagggg
     4141 ttattgcagg agttttcgct gtcatcgcag gaaggcgtgg cgctgatgtg tctggcagaa
     4201 gcgttgttgc gtattcccga caaagccacc cgtgatgcgt taattcgcga caaaatcagc
     4261 aacggtaact ggcagtcaca cattggtcgt agcccgtcac tgtttgttaa tgccgctacc
     4321 tgggggctgt tgtttactgg caaactggtt tccacccata acgaagccag cctctcccgc
     4381 tcgctgaacc gcattatcgg taagagcggt gaaccgctga tccgcaaagg tgtggatatg
     4441 gcgatgcgcc tgatgggcga gcagttcgtc actggcgaaa ccattgcgga agcgttagcc
     4501 aatgcccgca agctggaaga gaaaggtttc cgttactcct acgatatgct tggcgaagct
     4561 gcactaactg ccgcagatgc acaggcgtat atggtttcct atcagcaggc gattcacgcc
     4621 attggtaaag cgtctaacgg tcgtggcatc tatgaaggtc cgggcatttc aatcaagctg
     4681 tcggcgctgc atccgcgtta cagccgcgcc cagtatgacc gggtaatgga agagctttac
     4741 ccacgtctga aatcactcac tatgctggcg cgtcagtacg atattggtat caacattgac
     4801 gccgaagagg ccgatcgtct ggagatctcc ctcgatctgc tggaaaaact ctgtttcgag
     4861 cctgaactgg ctggctggaa cggcatcggt tttgttattc aggcttatca aaaacgctgc
     4921 ccactggtga ttgattacct gattgatctc gccacccgca gccgtcgccg tctgatgatt
     4981 cgcctggtga aaggcgcgta ctgggatagt gaaatcaagc gtgcacagat ggacggcctt
     5041 gaaggttatc cggtttatac ccgtaaggtg tataccgacg tttcttatct cgcctgtgcg
     5101 aaaaagctgc tggcggtacc gaatctaatc tacccgcagt tcgcgacgca caacgcccat
     5161 acgctggcgg cgatttatca actggcaggt cagaactact atccgggtca gtacgagttc
     5221 cagtgcctgc atggtatggg cgagccactg tatgagcagg tcaccgggaa agttgccgag
     5281 ggtaaactta accgtccgtg tcgtatttat gccccggttg gcacacatga aacgctgctg
     5341 gcgtatctgg tgcgtcgcct gctggaaaac ggtgctaaca cctcgtttgt taaccgtatt
     5401 gccgacacct ctttgccact ggatgaactg gtcgccgatc cggtcactgc tgtagaaaaa
     5461 ctggcgcaac aggaagggca aactggatta ccgcatccga aaattcccct gccgcgcgat
     5521 ctttacggtc acgggcgcga caactcggca gggctggatc tcgctaacga acaccggctg
     5581 gcctcgctct cttctgccct gctcaatagt gcactgcaaa aatggcaggc cttgcccatg
     5641 ctggaacaac ctgtagcggc aggtgagatg tcgcccgtta ttaaccctgc ggaaccgaaa
     5701 gatattgtgg gctttgtgcg tgaagccacg ccgcgtgaag tagaacaggc gctggaaagt
     5761 gcggttaata atgcgccaat ctggtttgcc actcctccgg ctgaacgcgc ggcaatttta
     5821 catcgcgctg ccgtgctaat ggaaagccag atgcagcaac tgattggtat tctggtgcgt
     5881 gaagccggaa aaaccttcag taacgccatt gccgaagtgc gcgaagcggt cgattttctc
     5941 cactactacg ccggacaggt gcgggatgat ttcgctaacg aaacccaccg tccattaggg
     6001 cctgtggtgt gtatcagccc gtggaacttc ccgctggcga ttttcaccgg gcagattgct
     6061 gccgcactgg cggcaggtaa cagcgtgctg gcaaaaccgg cagaacaaac gccgctgatt
     6121 gccgcgcaag ggatcgccat tttgcttgaa gcgggtgtac cgccaggcgt ggtgcagttg
     6181 ctgccaggtc agggggaaac cgtgggcgcg caactgacgg gtgatgatcg cgtacgcggg
     6241 gtgatgttta ccggttcaac cgaagtcgct acgttactgc agcgcaatat cgccagccgc
     6301 ctggacgctc agggtcgccc tattccgctc atcgctgaaa ccggcggcat gaacgcgatg
     6361 attgtcgatt cttcagcact gaccgaacaa gtcgtaatag atgtgctggc ctcggcgttc
     6421 gacagtgcgg gtcagcgttg ttcggcactg cgcgtgctgt gcctgcaaga tgagattgcc
     6481 gaccatacgt tgaaaatgct gcgcggcgca atggccgaat gtcggatggg taatccgggt
     6541 tgcctgacca ctgatatcgg tccggtgatt gatagcgaag cgaaagccaa tatcgagcgt
     6601 catattcaga ccatgcgtag caaaggccgt ccggtgttcc aggcggtacg ggaaaacaac
     6661 gaagatgccc gtgaatggca aagcggcacc tttgtcgccc cgacgctgat cgaactggat
     6721 gactttgccg aattgcaaaa agaggtcttt ggtccggtgc tgcatgtggt gcgttataac
     6781 cgtaatcagc tgccggagct gatcgagcag attaacgctt ccggctatgg tctgacgctt
     6841 ggcgttcata cgcgtattga tgaaaccatc gcccaggtca ctggctcagc ccatgttggt
     6901 aacctgtatg ttaaccgtaa tatggtgggc gcagtggttg gggtgcagcc attcggcggc
     6961 gaagggttgt ccggtaccgg gccgaaagca ggcggtccgc tctatctcta ccgtctgctg
     7021 gcgaatcgcc cggaaagtgc gctggcggtg acgctcgcgc gtcaggatgc agagtatccg
     7081 gtcgatgcgc agttgaaagc cgcattgact cagccgctaa atgcactgcg ggaatgggca
     7141 gcaaatcgtc cagaattgca ggcgttatgt acgcaatatg gcgagctggc gcaggcagga
     7201 acacaacgat tgctgccagg gccgacgggt gagcgcaaca cctggacgct gctgccgcgt
     7261 gagcgagtat tgtgtatcgc ggatgatgaa caggatgcgc tgactcagct cgccgccgtg
     7321 ctggcggtgg gcagccaggt gctgtggcca gatgacgcgc tgcatcgtca gttagtgaag
     7381 gcattgccat cgactgtcag cgagcgtatt caactggcga aagcggaaaa tattaccgct
     7441 caaccctttg atgcggtgat cttccacggt gattcggatc agcttcgcgc attgtgtgaa
     7501 gcagttgccg cgcgggatgg cgcaattgtt tcggtgcagg gttttgcccg tggcgaaagc
     7561 aatatccttc tggaacggct gtatatcgag cgttcgctga gtgtgaatac tgctgccgct
     7621 ggcggtaacg ccagtttaat gacgatcggt taaacaggta gccggaggat gttactccat
     7681 cctccggcat cattagcgtg gtcgaatccc ctcaataata atccgctgca cgttttcaac
     7741 cgtttgattg aaaaatacct catcgcgcaa cgttgcgcct gtcactgcct ccacctgagg
     7801 ggcaaaatcg gcgtaatgtt gagtggaagc ccaaatcata aaaatcaaat gctgcggatc
     7861 aattggcgcg agtttgccgc ttttgaccca accggcaatc agcgccgatt tctcatcaat
     7921 taatgccttc aaatcgcccg tcagttcatc cattaacagc ggcgcgcctg ccagcatctc
     7981 catacagaac aggcgcgaag cctgcggata atcgcgtgag acttccagct tcagacggat
     8041 gtactctttg atcgccgcca gcggggcgaa atcttcacga aacgctttta acggtgccag
     8101 ccagatatcg agaatctgcc gcagcacggc aatatacagc gcctcttttg acggaaagta
     8161 atacagcaga ttggtttttg aaacacccgc caactctgcg atctgctcca gccttgtgcc
     8221 gtgaaaaccg aattgtgaaa aagtgtccag tgctgcgcta agaatcgctt ttttcttcgc
     8281 gcttactgcg cgcgaacgtt tacccgttgt tttcactgcg ccttgcgtca tgcgctctcc
     8341 cctcttgtag attggcaaca ggatagcaaa acccccgccc ggcctcgacc ttctgcactc
     8401 tcatcgcgca cggatgcatg ttttatgtgc aactgttttg accgtttagt ccacttttta
     8461 ccagatattt tagataacgc aatgttaaca cattaataac attcactttt taaaaactgg
     8521 catccgcttt gcaaacaagc caatacgcgg ctgaaaagga tatgcaggat gcagcgcccc
     8581 gtctgacttt cactttacga gacgaagaga ggttgatgat gaaaattggc gtattcgtac
     8641 ctattggcaa caacggctgg ctcatttcga cccacgcgcc gcagtacatg ccgacctttg
     8701 aactgaataa atccattgtg caaaaagcgg agcactacca tttcgatttc gccctgtcga
     8761 tgatcaaact gcgtggcttt ggcggcaaaa ctgagttctg ggatcacaac cttgagtcgt
     8821 tcaccttgat ggcggggctg gcggccgtga cctcgcgcat tcagatttac gccactgccg
     8881 ccaccttaac gttacctccg gcaatcgtcg cccgtatggc cgcaaccatc gactcaatct
     8941 ctggcggacg ttttggcgtc aacctcgtga ctggctggca aaagcccgag tatgagcaga
     9001 tgggtatctg gcctggcgat gactatttct cccgtcgtta cgactatctc accgaatatg
     9061 ttcaggtgct gcgcgacctg tggggttcgg ggaaaagcga ttttaaaggc gattttttca
     9121 ccatgaatga ttgtcgcgtc agtccgcaac cgagtgtccc catgaaagtg atctgcgccg
     9181 ggcaaagcga cgctggcatg gcgttctccg ctcagtatgc cgatttcaac ttctgtttcg
     9241 gcaaaggcgt aaatacacct acggctttcg ccccgaccgc tgtgcggatg aaacaggccg
     9301 cagagcaaac cgggcgcgac gttggctctt atgtgttgtt tatggtgatt gccgatgaaa
     9361 ccgacgatgc cgctcgtgcc aaatgggaac actacaaagc gggcgcggat gaagaggcgt
     9421 taagctggct aaccgaacaa actcagaaag atacccgctc cggtactgac accaacgtcc
     9481 gtcagatggc cgatcccact tcagcggtaa acatcaatat ggggacgtta gtcggttctt
     9541 acgccagtgt cgcgcgcatg ttagatgaag tcgcaagcgt gcctggtgcc gaaggcgtgc
     9601 tgttaacctt cgacgatttt ctgtcgggaa tcgaaacctt cggcgagcgc attcaaccac
     9661 tgatgcagtg ccgcgcccat ctccctgtgc tgactcagga ggtggcatga tgacgacatt
     9721 aaccgctcga ccggaagcca ttaccttcga tccgcagcaa agtgcgctga tcgtggtgga
     9781 tatgcaaaac gcctatgcca cgccaggcgg ctacttagat ctcgccgggt ttgatgtctc
     9841 aaccactcgc ccggtcattg ccaacattca aaccgccgtg accgcagcgc gagcggcagg
     9901 gatgttgatc atctggtttc aaaatggctg ggatgaacag tatgtcgagg ctggcggccc
     9961 cggctcaccg aattttcata aatcgaacgc cttgaaaacc atgcgtaagc agccgcagct
    10021 acaggggaaa ttgctggcga aaggctcctg ggattatcaa ctggtggatg aactggtgcc
    10081 gcagcctggc gatattgtgc tgccgaagcc gcgctacagc ggtttcttca atacgccgct
    10141 ggacagcatt ttgcgcagcc gcggaatacg ccatctggtt ttcaccggca tcgctaccaa
    10201 cgtctgcgtc gaatcgacgc tacgcgacgg cttttttctg gagtatttcg gcgtggtgct
    10261 ggaagacgca actcaccagg cggggccgga atttgcgcag aaagctgcgt tgttcaatat
    10321 cgaaaccttt tttggctggg tcagcgacgt cgaaacgttc tgcgacgcgc tttctcccac
    10381 gtcctttgct cgtatcgctt aaggagttta acgatgccaa aatccgtaat tattcccgct
    10441 ggcagcagcg caccgctggc ccccttcgtt cccggcacgc tggctgatgg cgtggtgtat
    10501 gtctccggta cgctggcttt tgatcaacat aataacgtgc tgtttgccga tgacccaaag
    10561 gcgcaaaccc gccacgttct ggaaaccatc cgcaaggtga tcgagacggc gggtggcacg
    10621 atggcggatg tgaccttcaa cagcatcttt attaccgact ggaaaaatta cgccgcgatt
    10681 aacgaaatct acgccgagtt ttttccaggt gataaaccgg cgcgattctg cattcagtgc
    10741 ggactggtaa aacctgacgc gctggtggaa atcgccacaa ttgcgcatat cgccaagtga
    10801 ggccgcgatg aaactttcac tctcacctcc cccttatgct gatgcgcccg tagtggtgtt
    10861 gatttcgggt cttggcggta gcggcagtta ctggttaccg caactggcgg tgctggagca
    10921 ggagtatcag gtagtctgtt acgaccagcg cggcaccggc aataatcccg acacgctggc
    10981 agaagattac agtatcaccc agatggcagc ggaactgcat caggcgctgg tagccgcagg
    11041 gattgagcat tacgcagtgg tcggccatgc gctcggtgcg ctggtgggaa tgcagctggc
    11101 gctggattat cccgcgtcgg taactgtgct ggtctgcgtt aacggctggc tacgaataaa
    11161 cgcccatacg cgccgctgtt ttcaggttcg cgaacgatta ctgtatagcg gtggcgcgca
    11221 ggcatgggtg gaagcgcagc cgttgttcct ctatcccgcc gactggatgg cggcccgcgc
    11281 acctcgcctt gaggcagaag acgcgctggc actggcgcat tttcagggca aaaataattt
    11341 actgcgtcga cttaacgccc tcaaacgcgc tgactttagt caccatgcgg atcgcatccg
    11401 ctgcccggtg caaatcatct gcgccagtga tgatctgctg gtgccatcag catgttccag
    11461 tgaacttcat gccgccctgc ccgatagcca gaaaatggtg atgcgctatg gcggacacgc
    11521 ctgcaacgtg accgatcccg aaacgtttaa tgctctgtta ctcaacgggc ttgccagcct
    11581 gttacatcac cgtgaagccg ccctgtaagg aattgctatg aacgaagccg ttagcccagg
    11641 tgcgcttagc accctgttca ccgatgcccg cactcacaat ggctggcggg agacacccgt
    11701 cagcgatgag acgttacggg agctttatgc cctgatgaaa tgggggccga catcagctaa
    11761 ctgttctccg gcacggatcg tgtttatccg cactgcagag ggaaaagaac gtctgcgccc
    11821 ggcgctttcc agcggcaatc tgcaaaaaac cctgaccgcg cccgtcaccg ctatcgtcgc
    11881 atgggacagt gaattttatg aacggttacc gcaactgttt cctcacggcg atgcccgcag
    11941 ttggtttacc tccagcccac aacttgccga agaaacagcg tttcgcaaca gttccatgca
    12001 ggcggcttat ctgatcatcg cctgccgtgc gctggggctg gataccggcc cgatgtcggg
    12061 ttttgatcgt caacacgtag acgacgcttt ttttgcaggc agcacgctga agagcaatct
    12121 gctgattaat atcggctatg gcgatagaag caaacttttt gcgcgcctgc cacgtctgtc
    12181 ctttgaagaa gcctgcgggc tgttgtaagg agcaaactat gaacattgtc gatcaacaaa
    12241 cttttcgcga tgcgatgtcc tgcatgggtg cggcggtcaa tatcatcacc acggacggtc
    12301 cagccgggcg cgccgggttc accgccagcg ccgtctgcag cgtgaccgac acgcctccca
    12361 cattactggt gtgcctgaat cgtggggcgt ccgtctggcc ggtattcaat gaaaatcgaa
    12421 cgctgtgtgt aaatacactt agcgccgggc aggagccgct ttcaaacctt tttggcggca
    12481 aaacgcccat ggaacaccgc tttgccgccg cccgctggca gacgggtgtg accggatgcc
    12541 cgcaactgga agaggcgctg gtttcgtttg actgccgtat cagccaggtg gtgagcatcg
    12601 gcacccacga cattctgttt tgcgccatcg aggcgattca tcgtcatgcc acaccctacg
    12661 ggctggtgtg gttcgatcgc agttatcacg cgctgatgcg ccctgcttgt taacctcctt
    12721 aaggagacag ctgatggcaa tgttcggttt tcctcactgg cagttgaaat cgacctctac
    12781 agagagcggc gtggtcgcgc cggatgaacg attgccgttt gctcagacgg caataatggg
    12841 cgttcaacac gcggtggcga tgtttggcgc gacggtgtta atgccgattc tgatggggct
    12901 ggatcccaat ctttccattt taatgtcagg gatcggcacg ttactgtttt tctttattac
    12961 cggcgggcgc gtgcccagtt atctcggctc cagcgcggct tttgtcggcg tggtgatcgc
    13021 cgcaacgggc tttaacggtc agggcattaa cccgaatatc agcattgcgc tgggcgggat
    13081 tatcgcctgt gggctggttt ataccgtcat tggcctggtg gtggtgaaaa tcggcacgcg
    13141 ctggattgaa cggctaatgc cgccggtggt gacgggtgcg gtggtgatgg cgattggcct
    13201 gaacttagcg cctatcgcgg taaaaagtgt atcggcttca ggctttgata gctggatggc
    13261 ggtaatgacg gtgctgtgta ttgggctggt ggcggtgttt acccgtggca tgatccagcg
    13321 gttattgatt cttgtaggat taattgtcgc ctgtctgctg tatggcgtga tgaccaacgt
    13381 tttagggctg ggaaaagccg tcgattttac tctcgtcagc catgctgcct ggttcggtct
    13441 gccacatttt tccactcctg cttttaatgg tcaggcaatg atgctgattg cgcccgtggc
    13501 ggtgattctg gtggcggaaa acttaggtca tctcaaggcg gtcgccggga tgaccggacg
    13561 caatatggac ccgtatatgg ggcgagcatt cgtaggcgat ggtctggcaa cgatgctttc
    13621 tggttctgtc ggtggcagcg gcgtaaccac ctacgccgag aatatcggtg taatggccgt
    13681 gacaaaagtc tactcgacgc tggtgtttgt cgctgctgcc gtcatcgcca tgctgttggg
    13741 cttttcaccg aaatttggcg cgctgattca taccattccg gccgcggtga ttggtggtgc
    13801 atcaattgtt gtgttcgggc tgattgccgt cgcaggcgca aggatctggg tacaaaaccg
    13861 tgtcgattta agccagaacg gtaatttgat tatggtcgca gtaaccttag tgctgggcgc
    13921 gggtgatttt gctctcacgc tgggcggttt tacgttggga ggaatcggta cagcaacctt
    13981 tggcgcgatt ttactcaatg cgttattaag cagaaggttg gttgacgttc caccgccaga
    14041 agttgttcat caggagccat aaacagaaaa ccgccgggat taccggcggt gatgactttt
    14101 tgacgagctt aagaaaaaaa tgcattcgtc attgattagc ccggctagtt gtcgcttttg
    14161 ccgtgactgc tcttgccacc ttttttacct gcttcagatg cgcgctgcgg atcattcttg
    14221 aaattacccc cgctgtgctg tccacctttt ttacctgctt ctgatgctct ttcgcggtct
    14281 tctgcaaaat tgccggaacc gcctcgatgg tttgccatta ctgacctccg tctgggtata
    14341 gacatcatat tgcatagaat taacgcggcg tatggcggct gccatatttt cgctacgtgg
    14401 gactaagctt agtgaactga cgtgtttcta cccgcgtttg gtaatatcct gcaacagctt
    14461 cttagcgaga atagatatca tctccaataa ttatccataa gccgctctta tgattaaaaa
    14521 aacgcgatga gataaaaatg tgtcattttg cgacaaaatt gcgtgcttgt gaaagttgtt
    14581 ataaatcaaa taagtggttg tgaaatttgc actctgaaaa ggacgtctta tctttaaata
    14641 aatggtagcg aatcgctacg gaatagagat aacacgagga gtggttagaa atggctaaag
    14701 ttctggtgct ttattattcc atgtacggac atattgaaac gatggcacgc gcagtcgctg
    14761 agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa gcgtgtaccg gaaaccatgc
    14821 cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac tgcaccggtt gcaaccccgc
    14881 aagaactggc cgattacgac gccattattt ttggtacacc tacccgcttt ggcaacatgt
    14941 ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct gtgggcttcc ggcgcactat
    15001 acggaaaact ggcgagcgtc tttagttcca ccggtactgg cggcggtcag gaacaaacca
    15061 tcacatccac ctggacgacc cttgcgcatc acggcatggt gattgtcccc attggctacg
    15121 cagcgcagga attatttgac gtttcacagg ttcgcggcgg tacgccgtac ggcgcaacca
    15181 ccatcgcagg cggtgacggc tcacgccagc caagccagga agaactgtct attgctcgtt
    15241 atcaagggga atatgtcgca ggtctggcag ttaaacttaa cggctaatct tcaacaggag
    15301 gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc ggtgaatggg catctctgcg
    15361 caatacgtcg ccggaaatag ccgaggccat tttcgaagtc gccgggtacg acgaaaaaat
    15421 ggcggaaaag atttgggaag aaggtagcga tgaagtgtta gtcaaagcct ttgctaagac
    15481 cgataaagat tcgctttttt ggggcgaaca gaccatcgaa cgtaaaaacg tttaaccagc
    15541 gactcccccg cttctcgcgg gggagttttc tgttatttca ccgcttcatt caacacgcta
    15601 tcaaacttat ccatcgggca gaaaccgttg gcatctatcg ggcaaccgct taattccagc
    15661 gtcacacgct gcgcaggtgc ctgcagggtt aacgcatcgg cattacgtaa ctgttccgca
    15721 ctctggtaca catattcaat tttcatcaaa tcgcgattgg ctttgctgtc atgccaacgc
    15781 tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct ggtcatgcaa ctgatacggt
    15841 ttgaaatcca gcgccgttaa cagagaggca atgttggagt catgcccaac taacaccgtg
    15901 atttttggtg cactggtgcg atcggtgacc agggctttgt cgatataact gaccagcggt
    15961 ttcgcaacat tgcgcgccac ttccggtgag gtaaacaggc tgtcctggta accgtttttc
    16021 agcttcgaca acaccttcca ttgctggtca gatttgattt ctccccaggc tacctgatcc
    16081 atcggaaaac cttcgtagta ttgcaaagta aacgcatcta ccagcgagtt gccgactttc
    16141 agcggcccgg aaacacctgg ttcttgttga tacttcgcgc taaaggtatt tttgccatcc
    16201 accagcgaac actgttgttt ctctttacag gcaggggaat ctttatagtt aacgattttt
    16261 tccagtagct ggtagctgtc ggtaagctgg agtttgctga gctctttctc cattgccgcc
    16321 accgcctgtt cactgaatgc ggcggaatca tcggtgatca ccgggttaaa ggttgggtcc
    16381 atggtgccca ttttttcctg gtgatgcaca ggaatatcac accccgggaa tgcgccggta
    16441 ataaagaact gtgcggtcgc gacagtacgt tgcagactat tggcataggc ataaacggtg
    16501 tccggcggcg ggcattcccc cgatttcacc attccctgct ctgccagcca ttcacgcatg
    16561 taatggccca tatacacttc gagcacgcca cctttggtgg tgagttgccc accggggacg
    16621 tcccattcgg gccatttatt cggcgtcgac tgctccagca cactgccatt gttcgccagc
    16681 ggcgcacgta agttatggcg gctcatcatg agcacttgct gtagctgata gccttccggt
    16741 acggtttgtg cctgagcatt tgaagcgagt aaaactatcc ctgccacagt tgcggcgatt
    16801 agcgttttgt tcattcctgg cacctctttt gttatcaatc actaaagagt gtgacagaaa
    16861 tatgactttt ttccgggaac aggttcgcaa aatggcttgt tgaacacctg tttttctata
    16921 tacatcttta aaaacaggca aactatgcca gattagcctc tgcctcgcgt acgccgctga
    16981 tgattacacc tgtggctaaa tagaatatca gcggcgttac taatacagag gcaatagtac
    17041 caataataag agaagtgagg ctaataacaa tggtttatag cacatgcctt tgtcattaaa
    17101 ttgcataacg atttaaatag tcaagttgcg cattcatttc cttattgttt aacttgtaag
    17161 cctgagcaaa aagttcagga gggaggtaaa cgtcataatg attgccagtg tttattaaat
    17221 aaccatactc aagatcttca tctgttgaaa aggattcatt ccctaaggag attgatgcta
    17281 cattactatt aacaataaat aatgagcaaa tgctatctga aagaacagaa agaaaattct
    17341 tcaccgcttt aatcctatcc gacgtgtcgg tatc
//
LOCUS       DAZFFG010000095        17073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFFG010000095 DAZFFG010000000
VERSION     DAZFFG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.095"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..333)
                     /locus_tag="ACHJ7J_003626"
     CDS             complement(<1..333)
                     /locus_tag="ACHJ7J_003626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001809.1"
                     /translation="MKGIIIKAISLSMLLVSSLVYACGDNPNAIVQGPFKDSVFNNGV
                     ICFQNSPDKKDIYFYQSYSSASGNINKIIDTFIYSDAPAEVSSVFFTTIAGKRNVVVL
                     LRWNVNYFT"
     gene            complement(335..2986)
                     /locus_tag="ACHJ7J_003627"
     CDS             complement(335..2986)
                     /locus_tag="ACHJ7J_003627"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pesticin C-terminus-like muramidase"
                     /protein_id="HFO5001810.1"
                     /translation="MTAKLPEISYPVPSNKNGHAFSSVEALLSMIEGESSGLYLVGSQ
                     GMWHGGIHITDATIPWCALSTDSEPEKEYCRELYKGEQFIRCMADGEIVAWRVCRDYE
                     SAAIEWRGEKLFASTSFVLVKHYIQPADNAESGLTFFTLYMNLAPWAAYGQQGRQADR
                     KVAGIQRYYTSAEDMQAGREAGKLNKDTLVTLSDAIVTRSRDRRQFTEVTITRETKNA
                     AGETLAAGTKVWTVSDRGSLRAIKSAPVPSWWAKCTPAYTTQPEGVVNCTSRTDWGYY
                     LSWEDVLHNNRAGELTAGFPLSYEPGNTAQQVIRPGRSPGDAARTFSLVTLGRDKDTL
                     KKGDRVWVVSDGDSLTPVAPAASGSEPVFNDVYVPPVPVTVSAGDNLGHMGFYQLPEE
                     NGKRSRYQVHIECLSMDDMEKFITNPGKAGEDAPVYLTWQTDAPLFDKGEQGMVAGNR
                     KTRASGILTLAKVLGVDTGGNTLTSNQDAVYYQIRPEGGWLPAASVKKVSQYALDELG
                     FVTLNKAPASFDLIDGVKRPDNVVKGILEQLYKAAKEEKRITHALNKYNYQRLLEMTD
                     SNEDGHYSEQEYLQAIHNVSYRDRLYRIIAKHASEWYYGKDEPLWKNYLDPLTRDVPL
                     WKTYLEAFLDKMTWMKAVSEKGVALGPEPWHMHPIVFLEAVAEKYGDTIDFNTTLGVY
                     RISKKSAEFILSWEAYVSTPYVPDGDQSSGVTVGYGYDLGQQTTSSARELLSAYYSNA
                     QVERLLTAIGKKGDNARAIVHGLVDITITKDKALDMAMVLKQKYCQKVVDIYPQAINL
                     PPDSAGAMLSLIYNRGASLALPKPNDPIDSRREMREIRDDFAQGNEIKIPSRLRSMKR
                     LWSNQRGLIRRREGEAQLIENDLGKDD"
     gene            complement(3002..5356)
                     /locus_tag="ACHJ7J_003628"
     CDS             complement(3002..5356)
                     /locus_tag="ACHJ7J_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5001811.1"
                     /translation="MNPLTSKADSVVLQLSGQSRYRVHVHECSHFLDVLRYSAVESLS
                     QLWRYDVVVTSSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRISA
                     SNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSREY
                     PPRELITQWWETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYEFGITLPL
                     RNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKSQDSTESIFSKEGITTGE
                     TYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYRVSGHSSSPYLTPGQVLETDGT
                     LPDVVRAGIVITTVCSRGSRKSCFTLTFEGIPYSETVCWRPALISRPVISGSLPARIE
                     SHEKNDIYAHLDDQGRYRVKLDFDRNSTEQGYAYLWLRLAKPCAGDTYGFHSPLIDGT
                     EVAIVFDGGDPDRPYIAYALHDSENPDHVTSDNHTRNVWRTPANNKLRMEDKRQEEHI
                     KLATEYGKTQLNMGHLVNSQREKRGAGFELRTDEHGAVRAAKGLFLTADAQARAQGPV
                     LEMAAAISQINQANSQMQALNSAAEAAGALLCDINTQISFVTDKIRELQSAVLLGSAP
                     QGVALTSGEHLQLCSTRNTMINAGQHLDIGAMKNLSVTVEKALGLFVHKDGAKMVANQ
                     GDIEIQAQHNTMALFSEKQLTVTSSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSE
                     GMMVMKVANYLVPGNGSTLPQETPDFKRTDVTVTTQNSAKWTSE"
     gene            complement(5353..8019)
                     /gene="tssH"
                     /locus_tag="ACHJ7J_003629"
                     /gene_synonym="clpV"
     CDS             complement(5353..8019)
                     /gene="tssH"
                     /locus_tag="ACHJ7J_003629"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5001812.1"
                     /translation="MENSAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYGWDMDTIWQSLLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLIRCDGLWPLLTLSQSQLQCLSPLLDAQSDERPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     TLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAA
                     NLLKPALARGELRTIAATTWTEYKQYFERDAALERRFQMVRVDEPDDETTCLMLRSLK
                     SRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQL
                     TRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARECE
                     LTGELLESRSDISRQSETHHLQQALHDIQKNQPLLSVDVDVRTVAGVVADWTGVPLSS
                     LMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLVGPSG
                     VGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAV
                     RKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDL
                     LMQQLTDKPETTESELHGLIRPLLRDHFQPALLARFQTVIYRPLAQSAMRTIVEMKLA
                     QVCERLYCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLGYH
                     AARQHPAYLSLTWNDEDGIVMVFDTQAEGDPS"
     gene            complement(8188..8679)
                     /gene="hcp"
                     /locus_tag="ACHJ7J_003630"
     CDS             complement(8188..8679)
                     /gene="hcp"
                     /locus_tag="ACHJ7J_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO5001813.1"
                     /translation="MAIPVYLWLKDDGGADIKGSVDVQDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYKINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPVYEKHNHPEQVELRYEKITWTYKDGNIIHSDAWSER
                     TTA"
     gene            complement(8685..10364)
                     /locus_tag="ACHJ7J_003631"
     CDS             complement(8685..10364)
                     /locus_tag="ACHJ7J_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5001814.1"
                     /translation="MRGSVIIPVALHVLVAVATLLWLLWYFIPAGNVFNGLTTLLILL
                     LAGWTVFKNCRNEKQKLTSDVTLTDAELALSDTRAPVVLVCGDMPEALFQDGPLRKTA
                     RGCWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYCCLPDWHHDEAVLRFTLKTLRQQ
                     CNQIKSLTGFALPVMLSAEFSGPETPWIIVRGDKPVVCPVYHAPQAFTDWLQVEDNIL
                     ALPTVSEAFSFIRNTLADELEKADRLTPPVRTFTVAMRLGAVLPGTPSVWSDWLCSRT
                     CLQFSRKPGQTVPAGMFPDAVLPLLVPFASTVQGGQSTRRLILLIWLCVLTALGISAL
                     NNRDLIRQVSTDLQRWNAVPMDHYRPKAESLAALKQDALLLEQWQRQGVPLRYSLGYY
                     PGQRLWLALQQAINTWVPPLPAPEPEAPPQIIRLDSLALFDAGKWTLKPGATKWLVNA
                     LVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGY
                     GESRPVAPNDTAEGRARNRRVEISLVPQADACRLPDASPAPEEGASENIME"
     gene            complement(10364..11053)
                     /gene="tssL"
                     /locus_tag="ACHJ7J_003632"
     CDS             complement(10364..11053)
                     /gene="tssL"
                     /locus_tag="ACHJ7J_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO5001815.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSKEGEEPDVDEGQVAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCYHRVTALGFQGVYCLKAVSQSQRDE
                     VMKALSERVSPPDAGLSLVVHRMEKHRWRLMRSVWFWIVLAVILTGVIWWGGHLWLQS
                     LLSSQIPELHG"
     gene            complement(11050..12399)
                     /gene="tssK"
                     /locus_tag="ACHJ7J_003633"
     CDS             complement(11050..12399)
                     /gene="tssK"
                     /locus_tag="ACHJ7J_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5001816.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGV
                     ETVEFNDGLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVV
                     IALPHMQPGVINVEQEGVSADRPVRYREEWVTLQDAFGSEEEPMAVARFNFTIRFAHE
                     NNDSWKVCPVARLIRDGQNAWRQDPSFIPPVASFGASPVLRERLVLLNRQLRSRRQRL
                     MAMRRESNERLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAG
                     SMLTFSLDHDPDAIPGYDHKDPASAFLPLFDLITGLLEASLPSRVIALEMSRPDGQTW
                     KASLHDIRLREEADLWLSVRSDIPAWQVAEKFPALCLAGSPDNVSEIYGVALRGIPLI
                     PVSRVPAALPVRMENQYFALDMESPAAHEMLEQGVCMFYVPELLGALELELFAVLRS"
     gene            complement(12415..13959)
                     /gene="tssC"
                     /locus_tag="ACHJ7J_003634"
     CDS             complement(12415..13959)
                     /gene="tssC"
                     /locus_tag="ACHJ7J_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5001817.1"
                     /translation="MLMSVQKEKNVAESVVSEAHAGDSVYASLFEKINLSPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGTQVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALVSAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFQKETMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPA
                     LYDTPDATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            complement(13981..14478)
                     /gene="tssB"
                     /locus_tag="ACHJ7J_003635"
     CDS             complement(13981..14478)
                     /gene="tssB"
                     /locus_tag="ACHJ7J_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5001818.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGAQKKVELPLKLLVAGDFSNR
                     QEERPLSERVKMDINKNNFNDVLADISPSVRLNVENTFAGDGSEESISLIFRNMNDFT
                     PEQVARQIPRLKAMLAMRSLLRDLKANLLDNVTFRKELEKILRDPALSQTLRDELRAL
                     VPEKA"
     gene            14985..15176
                     /locus_tag="ACHJ7J_003636"
     CDS             14985..15176
                     /locus_tag="ACHJ7J_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5001819.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPELREETE"
     gene            complement(15229..15462)
                     /locus_tag="ACHJ7J_003637"
     CDS             complement(15229..15462)
                     /locus_tag="ACHJ7J_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001820.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(15558..16181)
                     /locus_tag="ACHJ7J_003638"
     CDS             complement(15558..16181)
                     /locus_tag="ACHJ7J_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5001821.1"
                     /translation="MRIRIDYLADKYCFTELNESPRLRRQWQDVLEECRQTEAGPEER
                     LSIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(16270..16782)
                     /locus_tag="ACHJ7J_003639"
     CDS             complement(16270..16782)
                     /locus_tag="ACHJ7J_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001822.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPCLLAVHFTLHSAEGNING
                     QQVQTFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASATVDEECSQLVDLLQQTWRVISADGQCRVERCFWVVRPDISGQYVALKTVALSL
                     GLPVVTTISW"
     gene            16859..17073
                     /locus_tag="ACHJ7J_003640"
                     /pseudo
     CDS             16859..17073
                     /locus_tag="ACHJ7J_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4113 a         4825 c         4059 g         4076 t
ORIGIN      
        1 agtgaaataa ttaacattcc agcgcaaaag aacgactaca tttctcttgc cagcaattgt
       61 ggtaaaaaat acggaggaaa cctcagcggg agcatcagag taaataaatg tatcaattat
      121 tttgttaata ttcccgctag cggatgaata actttgataa aaataaatat cttttttatc
      181 tggagagttt tgaaaacaaa tcaccccatt attaaatacg ctatctttga atggcccttg
      241 aactattgca ttgggattat caccacaagc ataaaccaat gatgatacta aaagcatact
      301 taaggatatg gctttaatta taataccttt cattttaatc atctttacct aaatcgttct
      361 cgatcaactg ggcttcacct tcacggcgtc ttatcaaacc tcgctgattt gaccataagc
      421 gtttcatact tcgtaagcga ctcgggattt taatttcatt tccttgagca aagtcatctc
      481 gaatctctct catttctcgt cggctatcta tcggatcatt tggtttagga agagctaatg
      541 acgccccacg attgtaaatg agtgatagca ttgccccggc gctatccggt ggtaaattaa
      601 tggcctgtgg ataaatatcc acaactttct gacaatactt ttgttttaga accattgcca
      661 tatctaaagc tttatcttta gttatggtta tatctaccag cccatgaaca attgccctag
      721 cattatcacc ttttttccct attgcggtca gcaatctttc aacttgtgca tttgagtaat
      781 aagcacttag taattcgcga gctgatgatg tcgtttgttg ccctaaatca taaccatatc
      841 cgacagtcac tccgcttgac tgatccccat caggaacata gggtgtggaa acatatgctt
      901 cccaagataa aatgaattca gctgattttt ttgatatccg atacacacct aaagttgtat
      961 tgaaatcaat tgtatcacca tatttttcag caacagcctc caaaaacaca atcggatgca
     1021 tatgccacgg ctccggtccc agcgccactc ctttttcgga tactgctttc atccacgtca
     1081 tcttatccag aaacgcttcc agatatgttt tccataacgg cacatccctg gttaatggat
     1141 ccagatagtt tttccacagc ggctcatcct taccgtagta ccattcactg gcgtgtttcg
     1201 caataatacg gtacaggcgg tcacggtacg acacattatg aattgcctga agatattcct
     1261 gctctgagta atgtccgtcc tcgttactgt ctgtcatctc cagcagccgc tggtagttat
     1321 atttgttcag cgcgtgagtg atgcgttttt cctccttcgc tgctttatat aactgctcca
     1381 gaatcccctt caccacgtta tctggtcgct ttaccccatc aatcaggtca aagctcgctg
     1441 gtgctttatt caacgtcaca aaccccagct cgtctaacgc gtactgcgat acctttttta
     1501 cactggctgc gggtaaccag ccaccttccg ggcgtatctg ataatacacc gcatcctgat
     1561 tactggtcag cgtattcccc ccggtatcaa cgccaaggac ttttgccagc gtcagaattc
     1621 ctgaggctct tgttttccga tttcccgcaa ccataccctg ttccccctta tcaaataacg
     1681 gtgcatcggt ttgccaggtc agataaaccg gtgcatcttc tcctgctttt ccggggttgg
     1741 tgatgaactt ttccatatca tccatactca ggcactctat atgtacctgg tagcgcgagc
     1801 gtttaccgtt ttcttccggt agctgataaa agcccatatg gccgagatta tctccagcac
     1861 tgacagttac agggactggt gggacataca catcattaaa aacgggttca ctgccggacg
     1921 ctgccggtgc aaccggcgtc aggctgtcac catctgacac cacccatacc cggtcacctt
     1981 ttttcaacgt atccttatca cgccccagcg tgaccagact gaacgttctt gcggcatcgc
     2041 cagggctcct gcccgggcgg ataacctgct gcgccgtatt acccggttcg taagagagcg
     2101 ggaaacctgc tgtcagctcg cctgccctgt tattgtgcaa cacatcttcc cagctcagat
     2161 agtaacccca gtctgtacgc gaggtacagt tcaccacacc ttccggctga gtggtgtaag
     2221 caggtgtaca ttttgcccac caggacggaa ccggcgctga tttaatcgct ctcagagacc
     2281 ccctgtccga taccgtccag actttcgttc ccgctgcaag cgtctctccc gctgcatttt
     2341 tcgtctctct ggtaatggtc acttcggtaa actgacgcct gtctctgctg cgagtgacaa
     2401 tagcgtcact cagtgtgaca agtgtgtctt tattcagttt tccggcttca cggcctgcct
     2461 gcatatcttc cgcactggtg taatagcgct ggatacctgc cacttttctg tccgcctggc
     2521 gcccctgttg tccataagca gcccagggtg ccagattcat gtacagcgta aaaaacgtca
     2581 gtccactttc tgcgttatca gccggctgaa tataatgttt caccagcaca aaggatgtcg
     2641 aagcgaacaa tttttcacca cgccattcaa tcgcggcact ctcgtaatcc ctgcataccc
     2701 gccaggcaac gatttcacca tcggccatac aacgaataaa ttgctcccct ttataaagct
     2761 cgcggcagta ctctttttcg ggctcactgt ctgtactgag agcacaccag gggatcgttg
     2821 catcagtaat atgaataccg ccatgccaca ttccctggct tcccaccaga tacagccctg
     2881 agctttcccc ttcgatcatc gataacaacg cttccacgga ggaaaaggca tggccatttt
     2941 tgtttgacgg tacggggtaa ctgatttcag gtaactttgc tgtcataaat cccctttcat
     3001 gctattcact ggtccatttg gcggaatttt gggtcgtgac cgtaacgtct gtccttttaa
     3061 agtccggtgt ctcctgcggc agagtgctgc catttccggg caccagataa ttcgcgacct
     3121 tcatcaccat catcccttct gaaccgtgct ctatcccgtt cttgctcagt ctcaggtaag
     3181 acccgccacc gttcagcgtc agggtttccg gggtgctgat aataatttca tcttcgctac
     3241 tggtcaccgt cagctgtttc tcagagaaca gagccatcgt gttgtgctga gcctgaatct
     3301 caatatctcc ctggttggct accatcttcg ccccgtcttt atgcacaaac agaccaaggg
     3361 ctttttccac cgttactgac agatttttca tcgcaccgat atcaagatgc tggcctgcat
     3421 tgatcatggt gttacgggta ctgcagagct gaaggtgttc acctgaggtc agggccaccc
     3481 cctgcggcgc gctacccagc aggaccgcag actggagctc cctgattttg tcggtcacaa
     3541 aactaatctg ggtattgata tcacagagca gggcaccggc ggcttcggct gcgctgttca
     3601 gggcctgcat ctggctgtta gcctggttaa tctgacttat cgctgccgcc atttccagta
     3661 ccggtccctg ggctctggcc tgggcatctg ccgtcaggaa taaccctttt gccgcacgta
     3721 cagccccatg ctcatccgtt cgcagttcaa aaccggcacc tcgcttttca cgctggctgt
     3781 tgaccaggtg tcccatgttc agctgtgttt tgccatattc cgttgcgagc ttgatatgct
     3841 cttcctggcg cttatcctcc atccggagct tattgttcgc cggcgtgcgc cacacgttcc
     3901 gcgtatgatt gtcactggtg acatgatccg gattctctga atcatgcagg gcataagcga
     3961 tgtagggacg atccggatct ccgccatcaa atacaatggc tacttcagtt ccgtcgataa
     4021 gcggtgagtg gaaaccgtac gtatcaccgg cacagggttt agccagccgc agccacaaat
     4081 aggcataacc ctgttcagta ctgttgcggt caaagtccag cttcacccgg taccgtccct
     4141 gatcgtccag atgggcgtag atatcgttct tctcgtgact ctcaatccgc gccggaagcg
     4201 agccggatat caccgggcga ctgatcagtg ccggacgcca gcagaccgtt tcgctgtagg
     4261 ggatcccctc aaacgtcagc gtaaagcaac ttttgcgtga accgcgggaa cacaccgtgg
     4321 tgataacgat gcctgccctg acgacatcgg gtaacgtgcc gtccgtttca agtacctgac
     4381 ccggcgtcag ataagggctg gaggaatgcc cgctgacacg atactgcgcg ttcagaatac
     4441 gttcgtgacg cagacgggca aagtatgccc cggtctccgg gctttcagcg tcacctgccg
     4501 tcaggaaggg ttcagcataa tggtacgttt ctccggtggt gatcccttcc ttactgaaaa
     4561 tgctttcggt actgtcctga gatttcagcg cttcacggta gttgtagtcc cgggtgacca
     4621 cacttccgct caccacgtta caggtggtct gaatatccca gatgctctcc tgtccgctgt
     4681 cactcatccc ggcctgattg cgcaatggca gcgtgatgcc aaattcatac tgctgctggc
     4741 tgtcctggaa aatcactatg tcctgttcaa gacgactgtc catttcataa cgccagtaaa
     4801 tccccacctc cgccagcagg cgccggataa actcaaggtc agtttcccac cactgggtga
     4861 tgagctcccg gggtggatac tcccgggaga gccggaattc aaaatcctgc ccttccagac
     4921 catgtttacg caatacctgc tcaaccactt cagtgactga ctgattgaga tagatttcgc
     4981 tccctttcgt atagttcaga agggcgatac ggggaacgat cgtcagtgcg taacgggtct
     5041 catcattcga ggcagagata cggcgaaacg actcaaccac gccgaatacc gtcctgagcg
     5101 gcagggccgg tgttccgtca gacaccgggg tctggaaagt aaatgacgcc ggcttcagca
     5161 ataacgcgtc acaggcaata tccgcagagg aactggtgac aaccacgtca tagcgccaga
     5221 gctggctgag ggactcaacc gcgctgtaac gcaacacatc cagaaaatgg ctgcattcat
     5281 gcacatgaac ccggtaccgt gattgccccg aaagctgcag tacaacactg tcagccttgc
     5341 tggtcagggg gttcatgacg gatctccttc tgcctgtgta tcaaatacca tcacaatacc
     5401 gtcctcgtca ttccacgtca gagacaggta cgctggatgc tgtctggcag catgatagcc
     5461 cagcaactgc cgactcagta ccggcagcag ttgttggttc agcagactct ccacattccg
     5521 ggctcccgta tccggcagca ggcaggctga tgtcagggca tcgtacacac gttcatcaac
     5581 cgatgtgctc agtccataat ggcagtacag acgttcacag acctgtgcaa gcttcatctc
     5641 cacaatggtg cgcatggcag actgtgccag cggacggtaa atcaccgtct gaaaccgcgc
     5701 cagcagggct ggctggaagt ggtcgcgcag cagtggacgg ataagcccat gcaactcaga
     5761 ttcagttgtc tccggcttat cagtcagttg ctgcatcaga aggtcgctgc ctaggttgga
     5821 ggtcatcagt ataaccgtat tacggaagtc aatttcacgc ccctcgccgt cgcgcataaa
     5881 accccggtca aagacctgat aaaacagatt cagcacatcc cggtgggcct tttccacttc
     5941 atccagcagc accacgctgt aagggcgctt acgtacagct tccgtcagaa tccccccctg
     6001 accgtatccc acgtacccgg gcggtgaacc cttaagctgg gaaaccgtat ggggctcctg
     6061 atattccgac agattgatgg taatcagtga tttttcgccg ccgtacatca cgtctgccaa
     6121 cgccagtgcc gtttcggttt ttcccacacc actgggacca accagcagga acaccccctg
     6181 ggggccgtta ccggatgtca gacccgtttt cgccgcacgc agacgctgtg caatggattc
     6241 cagtgccacg tcctgtccga ccacccgtct gccgatatcc ttttccagat gcagcagttc
     6301 cgtctgttca tccttcatca gtgaggataa cggcactccg gtccagtccg cgaccacacc
     6361 ggcaacggtg cgtacgtcaa catccacact gagcaggggc tgattcttct gaatgtcgtg
     6421 cagtgcctgc tgcagatgat gtgtctcact ctggcgggaa atatcgctgc ggctttcaag
     6481 taactcaccg gtgagttcgc actcacgggc gtactgctgc tccaggtcat cacgtgctgt
     6541 catcagcacg ttctcttcct gctcaatggc agacaggcgt tcgccgtgat tctgatgacc
     6601 aacggcaata tcttccagca gtgcctgttt ttccataccg agggaagcga gctgtgaacg
     6661 gatacgggtc agctgttccg gcaccgtgtc gaggctcata cgcacacggg cagcagcggt
     6721 atcgagcagg tcaacggcct tgtccggtaa ctgacgttct gtcagataac ggcgcgagag
     6781 tgtgacggca gcacgtactg cctcatcggt gatatgcacg ttatgatgtt ccgcataacg
     6841 ggatttcagg gagcgcagca tcagacaggt cgtctcatca tccggctcgt ccactctgac
     6901 catctgaaaa cgccgttcca gggctgcatc acgttcaaag tattgtttgt actcagtcca
     6961 ggtggtggca gcaatggtgc gtagttcgcc ccgcgccagc gcaggcttca gcaggttggc
     7021 cgcatccgcg ccaccggcct gattaccggc accgataagg gtatgggctt catctataaa
     7081 cagcagtaca ggagccggtg actgctgcac ggcatcaatc acgtttttca ggcgctgttc
     7141 aaattcacct ttcacaccgg caccggcctg cagcagaccg aggtcgaggg tgcgcagggt
     7201 gacgggtctg agtgattccg gtacgttacc ttccgcaata cgcagggcga gcccttccac
     7261 cagagccgtt ttccccacac cggggtcccc caccagaatc gggttattct tgcggcggcg
     7321 ggagagaata tccaccatct gacggatttc cgtatcgcgc cccgataccg ggtcaatttt
     7381 accctctctc gcactggccg tgatgtcccg ggtgaattta tccagcgcac tctgtaacac
     7441 aggcggcagg tcacctgacg gtatacccgc cgtatccgca ccgacgggac gcccgataaa
     7501 ggtcacattg tctccctgag gcagcacagg ctctgcatcc tgtaatctct ccggacgctc
     7561 atcagactgc gcatccagca gcggggacag acactgcaac tggctctgac tcagtgtcag
     7621 caggggccac aacccgtcac agcgaatcag gtgcggattt tccgtcaggg cacccagcag
     7681 atgcacgctg cggatatgtt cttccccctg taatgaggcc accatccagg cctgtttcag
     7741 cagggcattc agggacggcg caagctgcgg gcgactgcgt acgctacggg gctggttatc
     7801 cagccagccg agcagcgact gccatatcgt gtccatatcc cagccataac gcctgcccag
     7861 cacggtaagg tctccttccc cctgctccag cagtttcagt aaccagtgct ccggggtgat
     7921 ttctgcatgg gctcgggtct ggcaaaggga ggctgcgcct tccagtgcac gggcacagta
     7981 atgattaaga cgacgtaaca gagctgccga attttccatg ttattcactc actgtcagga
     8041 gaccgtcagg cacgctaccg cccttcgccg taacccaggc gcatcaggtc tgtgttcagg
     8101 attgagaatt ctttcttcag cacccgccaa cagatactga agaaaaaata aaaatgacgg
     8161 acaggatacc ctgtccgtcc ggcagaatta cgcggtggta cgctcactcc atgcgtcgga
     8221 atgaatgatg ttgccgtcct tgtaagtcca ggtgattttc tcgtagcgca actccacctg
     8281 ttcagggtgg ttatgcttct cataaacggg attcttgatg tcgtgcatga ccgggttaac
     8341 cttcaccacc ttcacgttct caagcttcgt attgaagtac tccacctcct gaccggcatc
     8401 attgattttg taccacttaa attcagcaga cttcagggtc tgcccggtgg tcactgcctt
     8461 gtaaaggtac gggctggaag aatcaatttc cttggtaaac aggaacggcg tgtgaatacg
     8521 ggtaccagtc agcttaccgg tattgttatc cgtcggaatg tagaggcagt gctcctgagc
     8581 caccacctca atgctgccct cacggtcctg aacatccacg gaccctttga tatctgcacc
     8641 gccatcatct ttcagccaca gataaactgg aattgccata aaaattactc cattatgttt
     8701 tctgatgcgc cctcttccgg cgcaggggaa gcgtccggca gacgacaggc atcggcctgc
     8761 ggtaccagac tgatttcaac acggcggtta cgcgcacggc cttccgccgt gtcattcggg
     8821 gccacagggc ggctttcacc gtatccctgc acggcaaaac agctttgagg aatatcgccg
     8881 gtatcccgca tccagtcacg aaccgcctct gcacgcttca gggacagggc ctggttgcgc
     8941 tgcgggtcac cggtattgtc cgtgtggcca ctgaccacaa tcagccagcc tgcttttgcc
     9001 ttaatatcca ccagagcatt caccagccat tttgtggcgc ccggtttcag cgtccattta
     9061 ccggcatcaa acagcgccag actgtcgaga cggatgattt gcggaggtgc ttccggctcc
     9121 ggcgctggta aaggcggaac ccaggtattg atagcctgct gcagggccag ccacaggcgt
     9181 tgccccgggt aataacccag gctgtaacgt aaaggcactc cctgacgctg ccactgctca
     9241 agcagaagcg cgtcctgttt cagtgcagcc agcgattccg ccttaggacg gtagtgatcc
     9301 atcgggacag cattccagcg ctgtaaatcg gtgcttacct gacggatcag atcacgattg
     9361 ttaagcgcag aaatccccag cgcagtcaga acacacagcc atatcagcaa aatcagacga
     9421 cgggtactct gtcctccctg taccgtcgac gcaaaaggta ccagcagcgg cagaaccgca
     9481 tccgggaaca tccctgccgg cactgtctgc cctggttttc gggaaaactg caaacaggta
     9541 cgggaacaga gccagtcgga ccagactgac ggggtccccg gaagaacagc accaaggcgc
     9601 attgcaacgg taaatgttcg tacaggtggt gtcagccggt cagctttttc cagctcatca
     9661 gccagcgtat tacggataaa ggaaaatgcc tcgctgacgg tcggcagcgc cagaatattg
     9721 tcttccacct gcagccagtc agtgaacgcc tgaggtgcat gataaaccgg gcatacaacc
     9781 ggtttatcac cccggacgat tatccacggt gtttcaggcc cggaaaattc ggcactcaac
     9841 atcaccggca gtgcgaaccc cgtcagggat ttaatctgat tacactgctg gcggagagtt
     9901 ttcagtgtga aacgaagaac cgcttcatca tgatgccagt ctggcaggca gcagtacatc
     9961 acagagagct gaccaacctg gcgcgggaac tgcgtctgta tgctgcggac aacgtcagtt
    10021 aagcggctga cgtcaccgac ccgcagccag catccccggg ctgttttacg cagcggaccg
    10081 tcctgaaaca acgcttccgg catgtcacca cacaccagta caacaggggc ccgggtgtcg
    10141 gataatgcca gttcagcatc cgtcagggta acgtcagatg tgagcttttg cttttcattc
    10201 cggcagtttt taaaaacagt ccagcctgcc agaagaagga tcagcagagt agtcagaccg
    10261 ttaaaaacat tgcctgccgg aatgaaatac cacaacaacc acagcaacgt ggcaacagcc
    10321 accaggacgt gcagggctac cggaatgatg acagagccac gcatcagcca tgcaactccg
    10381 gtatttgtga tgacaaaaga gactggagcc agaggtgccc cccccaccag ataacgccgg
    10441 taagaatgac ggcgagcact atccagaacc agactgagcg catcagacgc cagcgatgtt
    10501 tctccatccg gtgaacaacc agggacagac cggcatcagg cggtgaaacg cgttcagaaa
    10561 gggctttcat gacctcatca cgctgcgact ggctgactgc tttcaggcag tacaccccct
    10621 gaaaacccag cgctgtcacc cggtgatagc aggttaaaac ggccgggtcc ggtgaaggct
    10681 gacgcagaac ctctgcaatg cgatcccaca gagcctcacc agcccgaagt gagccaaaaa
    10741 aacgggcctg aagcggcact ttacgccagg ccacctggcc ttcatcaacg tccggctcct
    10801 ctccttcctt gctcttttca ggcttacggc tcatcaccgt ttcgtccagg agggcacatt
    10861 gtgcataggt aatatgctcc acactggcat cgtcataacc ggcacgctcc agtgcttccc
    10921 gcacactttc gacctgttta acgcatttgt tgtaaagcgc agtgccatcg ggcgtgagtg
    10981 cacctttttt cagcatggtg acggtaagcc atgtctccat catcagctgg tcgatatcaa
    11041 tatcctgtct catgagcgca ataccgcaaa cagttcaagt tccagggcac caagtaactc
    11101 aggcacatag aacatacaca ctccctgctc cagcatttca tgtgcagcgg gactttccat
    11161 atcaagtgca aagtactggt tttccatgcg tacaggcaac gcagccggaa ccctgctcac
    11221 cgggatcaaa gggatccccc ggagtgccac accatagatc tcactgacat tatctggtga
    11281 ccccgccaga cagagtgccg gaaatttctc agcaacctgc caggccggga tatccgagcg
    11341 cacagacagc cagaggtcag cctcttcgcg caggcggata tcgtgaaggc tggccttcca
    11401 agtctgtcca tccggccggg acatttccag agcaatgacc cgggatggca gactggcttc
    11461 cagcagaccc gttatcaggt cgaacaacgg aagaaacgca ctggcaggat ccttgtggtc
    11521 ataacccggt atggcgtcag gatcatgatc cagggaaaaa gtcagcatac tgcctgccag
    11581 acgagcgagc tccgcccaca cctgttcagg tgggcgggaa ggaaaacgtt cgtattccgt
    11641 cagtacactg gcgtgggaat tcagcgcatt cagcaaccag aaaagggaaa catcagcaac
    11701 agcaaaatcg gcgagtcttt cgttactctc acgacgcatc gccatcagac gctggcgtcg
    11761 ggagcgtagc tgacgattga gcaacaccag acgttcgcgc agaaccggac ttgccccaaa
    11821 tgacgccacc ggaggaataa aagacggatc ctgtcgccac gcattctgac catctctgat
    11881 caatcttgct accgggcaga ctttccatga gtcattgttt tcatgggcaa accggatggt
    11941 aaaattaaac cgggcaacgg ccatgggctc ttcttcagag ccaaaagcat cctgcaacgt
    12001 tacccactct tcccgatagc gaacaggtct gtctgcactg acaccttcct gctcaacgtt
    12061 aatcacgcca ggctgcatgt ggggaagtgc gataacgaca gttaccgcat cgcggtcagc
    12121 cagctggccg gcatctaact cacgcggcgc cggaggcaga tcgctccttt gagcatcaat
    12181 aagtgttccg tcttccagcc agaggcgtaa ctgagtaatc tgaatcagcc cggacgacag
    12241 aagaccgtca ttaaactcaa ccgtttccac tccccaggga aacggggatg ccagcgcaga
    12301 aataccggca ctgcgaaaag actcccattc agcctgctgc tgaaactgct gaggggagag
    12361 caaaacacct tcgctccaca gtggacgata aatcttcatt tcccgacgcc tgccttacgc
    12421 ttttgccttc ggcatctgcg aaaccaatga caggctgacg tccatacctt ctacctggaa
    12481 atgcggtaca gcgtagagtt tcacgcggaa gaatcccgga ttgtcttcga tatcttccac
    12541 caccactttt ccgtcacgca gcggatgcga ggcctgcagt tcatcacccg gatcagtcat
    12601 ctccgtcacc agtccccgga tccagttatt cagctccagc tcaagcagac ggcgatcttt
    12661 ggtggtaccg atattttccc gctgaatgat cttcaggtaa tgcgcaatac gggacagcag
    12721 gaaaatatag ggaagacggg cattaatacg actgttcgcc gttgcatccg gcgtatcgta
    12781 cagcgccggt ttctgggcgg agttagcaga gaagaagcag gcataatccc ggttcttgta
    12841 gtaagagagc ggaataaagc ccagattcgc gaattcaaac tcccgcgttt ccgggatcat
    12901 cacctctgac ggaattttga cctgattacc tgttcccaga tcatacagat ggatcggcaa
    12961 atcctgtacc gcaccaccgg cctgaggtcc gcggatctga acacaccagc cgttggtgac
    13021 aaaactgcgc accatgttgg ctgcaaaggc aaaagatgcg ttcgtccaga gatatttatg
    13081 gtgatccggt cctttcacct cttcaacata gttaaagctg cggacaggta cggtatcagg
    13141 gccatacggc aggcgtccca gaacacgtgg catcaccagg ccaatataac gggcatcatc
    13201 cgtctcacgg aaagcgttcc acttaatgta ctcggcacgc tcaaagtggt tgccaatatc
    13261 cttaatagca gccacctctt ccatggtctc tttctggaag aaagccgggc caacagaacc
    13321 aataaacggc atatgtgcgg atgctgccac ccgggaaata tttttcagca gcgccacgtc
    13381 ctgagcagat gcatcaaatt cccaggccga aaccagggca gcaatgggct caccacccgg
    13441 cgtgtcatac tcagccacat aggtctgcag ataaaggccg ctctgaataa tttccggcgc
    13501 atcctcaaaa tcctgacgca gatcgtcttt ggacagatcc agcagctcaa ttttcacatt
    13561 acggcggaag tccgttttat ccaccagaga cttcacgccc cgccacaggg attccacttt
    13621 ctgaaattcc ggatgatgaa gcacggcatc cagttggcga ctgatctgat aatcaagctc
    13681 tgcaatatga tggtcaatca gcgctttatc gagtttttcc acctgcgtgc cggcttttgc
    13741 cagacactcc ataaacacct gcatccctgc cgtcagacgt tcatctgcag acgcttcgga
    13801 catggcctgt ggatcctgcc agatatccag tgcactcagg gcagataccg gactcaggtt
    13861 aattttttca aacagggaag catatacgct gtcgccggca tgcgcttcag ataccacgct
    13921 ctctgcgacg ttcttttctt tctgtacaga catcagcatt ttctctcgta atccgttaaa
    13981 tcacgccttt tccggcacca gtgcacgcag ttcatcacgc agcgtctgac tcagtgccgg
    14041 gtcacgcaga attttttcca gctctttacg gaaagtcacg ttatccagca ggttggcttt
    14101 cagatcccgc agaagactgc gcatggccag catggcttta agacggggaa tctgtcgggc
    14161 gacctgctct ggcgtgaaat cgttcatatt gcggaaaatc aggctgatgc tctcttcaga
    14221 gccatcaccg gcaaacgtgt tctccacatt cagacgcaca gacggagaga tatctgccag
    14281 aacatcatta aaattgtttt tattaatatc catttttacg cgctctgaca gtggacgctc
    14341 ttcctgtctg ttactgaaat caccggcaac caaaagcttg agaggaagtt ccaccttttt
    14401 ctgggctcct cccgtatgca ggtcaagttt taaatttacc cttgctttag ggatttcatt
    14461 ctgaaacgaa ctgctcatga ctctctcctg tgaaacctgc atctcagggg ttccatcacc
    14521 gcagatgcct ccccttgtgc actggtttaa ttttgatcat tatagaacga agatctgcaa
    14581 aatctcaata tgacagatac atcgatcgat ataatgcaaa tactcaggac tttattctat
    14641 gaaatgtcgc aaaacaatga atattcaaag cttataacca ttgttaatgt aaaaatacag
    14701 aaaattattc cacaaaacac ttttgataca gctagttaac ataaacactg tattttcttg
    14761 atttcagtca ttgaaaatca caatgagtaa agtttcagca gcattgacgt gatttctgga
    14821 aagcgggaaa gatatggagg aagggcacaa caaaatgcaa tgaaagaagg agtgagggga
    14881 tttctagtaa aaacagaaga ttaacaaaac acatcaggag gcagagactc ctgattcagt
    14941 gctaccacat gaaaatttag ttaactgtta ccgttctgaa atctatgtca gaagatattc
    15001 cattaccgaa aatcaatcag cgatacagag acgatcatgg tgcgcttgtc accgtgacca
    15061 gtgttgagga aacccgggtg gtgtttatgc gggacggcta tccgcatccc tgcatgcgac
    15121 cgatgtacaa cttcctggga aaattcaaac ccgaactccg ggaagaaaca gaataaaacg
    15181 aaatccctgc cggcaatcac actgcctgat ggcagcataa tccacttatt aattctcatc
    15241 cccggagaca agccctgaaa accactccgg gtgttcactg tatgctgtgt tcagcgcatc
    15301 ccaggaaacg ttgatataaa agctgtcctg caatttccgg gtaaccacct ctgccaggcg
    15361 gttccggtta tccaccagtc cggcataacc tctctctgcc agccactcat tcagttcatg
    15421 ttccagagac agattgataa tatcataatc ggaccttgcc attcccgttt ccttagcaca
    15481 tgttttcagg cacggggata aagtataaag aatgtcgtac tgctgctgcc agagcaatat
    15541 tccccctgat ggttgtatca gagatggtat gccggtactt tgcgggttgt tcctgtcagg
    15601 ttatcgtgca cctccacggt cgtggtcacc accggcattc cggcctttct cagtctcaga
    15661 acatcagccg caatacgctg actgccaaac cagaacaggc cgtccagtgc agtcaccagt
    15721 aaacccgcct ccagtgcata cttcagccgt tcacggggcg ctttcacttc ccgggcaacc
    15781 tgctgatatg gcgatgatgt gctttcattc ccaaccacgc gacgaatacg gtgagacaga
    15841 tgataccggt atgtatccgg cacaccagaa aggctggcct tcaggctgta cacgcaacca
    15901 aagcgtttat cgttgaacat cacatttttc tggctgatgt cccattcttc acgcagcgcg
    15961 gcaatcagtt gtggtgtacg ggtaagcaac aagcgaaaag gcagttcaaa actggtgacg
    16021 taatccacat tcagcagagc aatactcagc cgttcttctg gcccggcctc tgtctgccgg
    16081 cactcctcca gaacatcctg ccactgccgg cgaagacgag gggattcatt cagctctgta
    16141 aaacagtatt tatccgccag atagtcaatt cgtatacgca tactgaagaa aattccgtat
    16201 aaagattcag ctgacaaaac tttatcagtc cataagaatc aacggaagca cagatggttc
    16261 acacgtccat taccaactga ttgttgtcac aaccggtaac cccagagaca atgcgactgt
    16321 ttttaacgca acatactgac cagatatatc aggccggaca acccaaaaac acctttccac
    16381 ccggcattgt ccatcagcac ttatcacccg ccatgtctgt tgcaataaat ccaccagttg
    16441 cgaacattct tcatcaaccg tggcactggc tcgcggatgg caaatgctcg tctgactgag
    16501 cagcaacagg catcgtattg ttgctcctcc atgttgttcc cgcagccagc gtaataccac
    16561 cggcgatgat ggactggccg tgtgattacg ttcagtaata cgttcgtgta cctgtcggta
    16621 aaaggaagta cagaatgtct gaacctgttg tccgttgatg tttccttcag ccgaatggag
    16681 cgtgaagtgg actgccagca gacacggcca gtggtccacc gcctgctgaa caaaacgccg
    16741 gatgtctccc ggctctgaaa ttaaggcttc ggttatttgc acgaaatgtt ccttttcaaa
    16801 ccctgttgaa ttttgttaag ccagtgcaga cacagtaaag cgcgcatcaa cacctgaaat
    16861 gatagtttta ttcagagtat atgactttct tttacccgct caagatactt tccctagcga
    16921 tatcatcaga agtttatgtg ttgtgtatcc atccatatta atctggttcc agttttcggt
    16981 atatgtcatg aggaaacatt acaaatatat tctggagcgg agaaaggaaa ttgaaaccgt
    17041 ttcagaaagg ttgatacgtg aggctgatac gag
//
LOCUS       DAZFFG010000096        17042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFFG010000096 DAZFFG010000000
VERSION     DAZFFG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.096"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            93..1232
                     /gene="arnB"
                     /locus_tag="ACHJ7J_003641"
     CDS             93..1232
                     /gene="arnB"
                     /locus_tag="ACHJ7J_003641"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO5001823.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1236..2204
                     /gene="arnC"
                     /locus_tag="ACHJ7J_003642"
     CDS             1236..2204
                     /gene="arnC"
                     /locus_tag="ACHJ7J_003642"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO5001824.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2204..4186
                     /gene="arnA"
                     /locus_tag="ACHJ7J_003643"
     CDS             2204..4186
                     /gene="arnA"
                     /locus_tag="ACHJ7J_003643"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO5001825.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPVCTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4183..5073
                     /gene="arnD"
                     /locus_tag="ACHJ7J_003644"
     CDS             4183..5073
                     /gene="arnD"
                     /locus_tag="ACHJ7J_003644"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO5001826.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5073..6725
                     /gene="arnT"
                     /locus_tag="ACHJ7J_003645"
     CDS             5073..6725
                     /gene="arnT"
                     /locus_tag="ACHJ7J_003645"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO5001827.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLLGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6722..7057
                     /gene="arnE"
                     /locus_tag="ACHJ7J_003646"
     CDS             6722..7057
                     /gene="arnE"
                     /locus_tag="ACHJ7J_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO5001828.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7057..7443
                     /gene="arnF"
                     /locus_tag="ACHJ7J_003647"
     CDS             7057..7443
                     /gene="arnF"
                     /locus_tag="ACHJ7J_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO5001829.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWISSMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7437..7703)
                     /gene="pmrD"
                     /locus_tag="ACHJ7J_003648"
     CDS             complement(7437..7703)
                     /gene="pmrD"
                     /locus_tag="ACHJ7J_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO5001830.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7813..9168)
                     /gene="menE"
                     /locus_tag="ACHJ7J_003649"
     CDS             complement(7813..9168)
                     /gene="menE"
                     /locus_tag="ACHJ7J_003649"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO5001831.1"
                     /translation="MIFSDWPWRHWRQVRGETIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9165..10127)
                     /gene="menC"
                     /locus_tag="ACHJ7J_003650"
     CDS             complement(9165..10127)
                     /gene="menC"
                     /locus_tag="ACHJ7J_003650"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO5001832.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10127..10984)
                     /gene="menB"
                     /locus_tag="ACHJ7J_003651"
     CDS             complement(10127..10984)
                     /gene="menB"
                     /locus_tag="ACHJ7J_003651"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO5001833.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10999..11757)
                     /gene="menH"
                     /locus_tag="ACHJ7J_003652"
     CDS             complement(10999..11757)
                     /gene="menH"
                     /locus_tag="ACHJ7J_003652"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO5001834.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQRLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11754..13424)
                     /gene="menD"
                     /locus_tag="ACHJ7J_003653"
     CDS             complement(11754..13424)
                     /gene="menD"
                     /locus_tag="ACHJ7J_003653"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO5001835.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRVCDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTSTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13513..14808)
                     /gene="menF"
                     /locus_tag="ACHJ7J_003654"
     CDS             complement(13513..14808)
                     /gene="menF"
                     /locus_tag="ACHJ7J_003654"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO5001836.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14887..15192)
                     /gene="elaB"
                     /locus_tag="ACHJ7J_003655"
     CDS             complement(14887..15192)
                     /gene="elaB"
                     /locus_tag="ACHJ7J_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO5001837.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15247..15708)
                     /gene="elaA"
                     /locus_tag="ACHJ7J_003656"
     CDS             complement(15247..15708)
                     /gene="elaA"
                     /locus_tag="ACHJ7J_003656"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5001838.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15773..16690
                     /gene="rbn"
                     /locus_tag="ACHJ7J_003657"
     CDS             15773..16690
                     /gene="rbn"
                     /locus_tag="ACHJ7J_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO5001839.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIVQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16878..>17042
                     /locus_tag="ACHJ7J_003658"
     CDS             16878..>17042
                     /locus_tag="ACHJ7J_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO5001840.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIAL"
BASE COUNT         3955 a         4620 c         4357 g         4110 t
ORIGIN      
        1 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
       61 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
      121 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
      181 caaccggtcc gaagaatcac gcgcttgaac aagccttttg ccagttgacg ggaaatcagc
      241 atgccattgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
      301 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
      361 tttccttgca gggtgcaacg ccggtaatgg tggatgtcga tcgcgatacg ctgatggtca
      421 cgcctgaagc gatcgaatca gccattacgc cacgcactaa agccatcatt ccggtgcatt
      481 atgccggtgc gccagcagat attaacgcca ttcgcgccat tggcgaacgt tacggcatcg
      541 cagttatcga agatgcagcc catgccgtcg gtacgtatta caaagggcga catattggcg
      601 caaaaggtac cgctattttt tcgtttcacg ccatcaaaaa tattacctgt gcggaaggtg
      661 gcctgattgt tactgataat gaaaatcttg cccgccagct acggatgctg aaatttcacg
      721 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
      781 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
      841 tagctaaatt agagcacctc aacacccgtc ggcgcgaaat tgcccagcaa tatcagcaag
      901 cactggcagc tctccccttt cagccattaa gccttcccgc ctggtcgcac gttcacgcct
      961 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcga aatgcgttga
     1021 tggaagcgtt aaaagaaaga ggtattggta ccgggttaca tttccgcgcc gctcacacac
     1081 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
     1141 aacgcatctg ttcgttgccg ctgttcccgg atatgactac cgccgatgcc gaccgtgtca
     1201 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
     1261 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
     1321 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
     1381 gcagtagcga taattccgcg catatactgg tcgaagcctc acaagcggag aacagccata
     1441 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
     1501 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
     1561 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
     1621 agaaccgcca ggacagctgg tttcgcaaaa ccgcttcgaa gatgattaac cggcttattc
     1681 agcgcaccac cggcaaagcg atgggtgact acggttgtat gttgcgcgcc tatcgccgtc
     1741 atattgtcga tgcgatgttg cactgccatg aacgcagcac ctttatcccg attctggcga
     1801 atatcttcgc ccgccgtgcc attgaaattc cagtgcatca tgccgagcgt gagtatggtg
     1861 aatccaaata cagctttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
     1921 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
     1981 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
     2041 gtgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
     2101 gattgctcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
     2161 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
     2221 tgcctaccac gatatgggat gcctcggtat tgaagccctg ctggctgccg gttacgaaat
     2281 tagcgccatt tttacccata ccgataatcc cggtgaaaaa gccttttatg gttcggtggc
     2341 tcgtctggcg gcggaaagag gcattccggt ttatgcgccg gataacgtta atcatccgct
     2401 gtgggtggaa cgcattgccc aactctcacc agaggtgatt ttctcttttt attatcgcca
     2461 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc
     2521 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
     2581 agaaactggc gttacattgc accgaatggt aaaacgtgcc gatgccgggg ccattgtggc
     2641 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
     2701 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
     2761 ggaaatcgcc cagcgcgaaa acgaagccac ctgttttggt cgcagaacgc cggatgacag
     2821 tttcctcgaa tggcataagc cggcatccgt actgcacaac atggtacgtg ccgttgccga
     2881 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
     2941 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
     3001 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
     3061 tatgcagggc tcgcaattag cgcagacgct gggcctggtg caaggttcac gcttgaatag
     3121 ccagcctgtc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
     3181 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
     3241 ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga
     3301 aggcgatatc agtattcatt ccgaatggat tgagtatcat gtcaaaaaat gtgatgtcgt
     3361 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
     3421 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgtgaagt accgtaagcg
     3481 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
     3541 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtatc
     3601 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
     3661 cctcttccgc ccgtttaact ggatgggacc gcggctggat aaccttaatg cagcgcgaat
     3721 tggcagctcc cgcgcgatta cgcaactcat tctcaatctg gtagaaggtt caccgattaa
     3781 gctaattgat ggcggaaaac aaaaacgctg ctttactgat attcgcgatg gtatcgaggc
     3841 gttataccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
     3901 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
     3961 aaaacatccg ctgcgccatc atttcccacc gtttgcgggc tttcgcgttg tcgaaagtag
     4021 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
     4081 ccaccgctgc ctggactggg agccgaaaat tgatatgcag gaaaccatcg acgaaacgct
     4141 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtaggctta
     4201 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccgcgtct gctggaaatc
     4261 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
     4321 cgtcatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
     4381 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
     4441 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
     4501 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
     4561 gacgatattg cacgcggcct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
     4621 tctgccgctg cgggctggcg tgccgaccag caggtgatcg aagcaaaaga agcgttccat
     4681 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccactgct cgaatcagga
     4741 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
     4801 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
     4861 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
     4921 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
     4981 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
     5041 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc
     5101 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctatggcaac
     5161 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
     5221 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
     5281 gcattgggca atggctatta ggcgcgaata actttggtgt gcgggcaggc gttatctttg
     5341 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
     5401 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
     5461 cttatgccgt gctcgatccg tttattgcct tctggctggt ggcgggaatg tgcagcttct
     5521 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
     5581 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
     5641 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
     5701 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
     5761 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg
     5821 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
     5881 cgtggctggg attactcccc ggtgcactat acacaggctg gaaaaaccgc aagcattccg
     5941 caaccgtcta tttgttgagt tggacgataa tgccgctgct gtttttctcc gttgctaagg
     6001 gtaaattgcc cacctatatt ctttcttgct ttgcaccttt ggcaatgctg atggcgcatt
     6061 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
     6121 tcgcttttgg cgtcactggc attattgcca catttgtggt ctccccctgg ggaccaatga
     6181 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
     6241 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtcttttg
     6301 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
     6361 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
     6421 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
     6481 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
     6541 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
     6601 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
     6661 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
     6721 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
     6781 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
     6841 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcctgcagaa
     6901 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
     6961 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
     7021 tattggtggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc
     7081 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
     7141 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttgggct ggatgcccgg
     7201 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
     7261 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
     7321 atttcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
     7381 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
     7441 tgagttttcc ctgccgctgt acactgccgt tcccactcat cggggctata actgcttgcc
     7501 gagaggactt tcaccgtatg cagcttttca cgattaatac agtaaagcgc attctgcaaa
     7561 ggtgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
     7621 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
     7681 gattttttga ccagccattc cattgtatta tcctgtttgc taagagtttt catatcaatt
     7741 tacagattaa cagataaact ctttatcaac ggcaatggaa tggcagaact gagaagtgaa
     7801 acggagggtt aattattgct gacgttgcac ccactctttt agcgcctgac gtgaaatttt
     7861 aataccgccg tttttcagtt caggcggcaa cgttaaccaa cgcaccggtt gctgaaaacg
     7921 ggccagctta tctttcaccc attcactaag atcaacgctc tcgtggtcat actccatcac
     7981 cgccaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca cctgcaaaac
     8041 cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct ctccgccact
     8101 gaagaataga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
     8161 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
     8221 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
     8281 ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg cacacaccgt
     8341 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
     8401 ttccgtcaac tcgaccggga tagccgcacc gccaagtaac accgctttca gggaaacgga
     8461 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
     8521 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
     8581 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
     8641 atcgtgatcg ccaaacggaa tcaacgataa caccccttcg gcactggcaa gatgggcctg
     8701 ataagtatgg acagcggctt tcggcaaccc ggtagaaccc gaggtcaacg tcattgagca
     8761 cagacgcgtc ggctgccacg cagcggcatg tgcgccttca accagctgaa tgtgcagcga
     8821 cgttaacgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
     8881 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
     8941 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
     9001 catcacgccg ctgccctcaa caacgccctg cgccgcaaag ccggaggcta attcatcgac
     9061 gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg cgatggtttc
     9121 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caaccgctcc
     9181 agtgcatcaa cttccacgac aggcagcgtg ctacccggcc agcgacgtac ctgctgcgcc
     9241 tgcatcagat ccagcgtgtc cagccctgga atggtgtccg gcgttaacca ggcagcaatc
     9301 cgcgccagtt gcgttaagcc taagctcgat tcaatggaag aactgatcac cgccgtcagc
     9361 cccagcgcgt gcgccgcctg tacctgctcg cgaactttct ccagactgcc cgtgagcgtg
     9421 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
     9481 aagctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
     9541 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
     9601 ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
     9661 agatccggaa ttgcctccag caacagattc accaccatgc cgtcgcgcac ggcttcgtac
     9721 aatcccactt tgaccttcgc cactttctcg cctggcatat ctgcaagttt gaggatcaga
     9781 tcgtccggat cgccattaca cagcggtgcc gcacggtagt tggctgcttg cggcaacgta
     9841 tctgtcagtt ctgccaatgc acagcttacg ccaaaggcca cggaaggcat ctgcggtagc
     9901 tcgcaatcgc ctgccagcca gttatttacc caggcaagca gcacgctttg cgcctcttcc
     9961 caggtttcct ggctgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgcct
    10021 tcacgcaggc aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccacc
    10081 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    10141 tgaatttgct gaagtcaggc tgacgtttct ggttgaaggc gttgcgacct tcctgacctt
    10201 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcaga cctgcctgac
    10261 cgtcacagtc ggcgttcagt gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    10321 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    10381 tcacaaggcc catatccagc gcctgttttg cgtcgtactg acggcacagg aaccagattt
    10441 cacgcgcttt tttctgcccg acgatgcgag ccatgtagga agcgccccag ccgccgtcga
    10501 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgccgcg atagtcaggt
    10561 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    10621 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc
    10681 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    10741 acgctttatc gcctgcgcca gtcagaatga tcacgccgac gttatcgtca taacgcgcat
    10801 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    10861 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcga
    10921 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa
    10981 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga ttacgccagc
    11041 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    11101 ttccgccgcc agggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    11161 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    11221 catggcggca agcgttgcgc cattattgtt gctgcgcagc gccaccagct cccggcgttg
    11281 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgcggttaa
    11341 tggttcggaa cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg
    11401 ttcagcattt tgcagccccg gatgaccgcc ttcgacaaca accccacaaa gccccgccag
    11461 cctctggcaa gccgccatca tcgccacccg tccgccaagc gagtacccca ccagccagaa
    11521 gttaaggatg ttgtaactaa ccaaggtttt acgcagtaag tcggtgacat catcaaatcc
    11581 atcgacgcta atcgccgccg aaccaccgtg acctgggaga tcaacataca accgtgagta
    11641 gtcggcaaac acctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    11701 aaacaccagc cagggtaaac ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa
    11761 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    11821 atttcaatca ccgtggaggt tggcgtgcgc caggcgtcag caagcgccgt ttcaagttcc
    11881 tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    11941 acgttttgcg gcatcagata gaaacgctcg cgctcgcttt gcggcgttgg caacagcgag
    12001 aaaatttgcc cgccgttgtt gttcaccaca attaatacca gcggcgcgga aacctggcgc
    12061 aataacgcca gcgcattcag gtcgtaaagt gcggagagat cgcccacaat cgccagcgtc
    12121 ggtttgccgc ttgcccgctg aacgccggcg gcagtcgaga gcagcccgtc gataccgctg
    12181 gccccacggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga
    12241 cgtaccacca ggctgttacc gacaaacaat tgcccctgtt caggcagata gtcgcagacg
    12301 cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg caataaccgc ctgcatcgcc
    12361 tgctccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagt
    12421 tccagccagt cggcaatatt ggcaattaac cgacgcccgc gatggtgcgc cggatcaagc
    12481 cgcccttcga tgtcatccac aatccagtac tcttcgggtt cacagcttgc ctgccattgc
    12541 aggagccgtt tgccagtcag gctgcttccc agttgcacca caatttgcgc ctgttgcagt
    12601 tcgctggtcg ctttggcatt gcctaaccag agatcggcac acggcagcgg ctgtccggtt
    12661 tgcgacagca cgtcgccaat cagcggccag ccaagagttt gcgcccacag ggcaactttt
    12721 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgcttttg ccgccagaag
    12781 aaccagtcgc gctgtttttc gctctcaaga cgaggcgctt cacgcagcca cggcttatca
    12841 tcctgccacc agtcacccag acgctgttgc cagctaagcc cagtgtcgtc catctcgcca
    12901 tacagcggtt cagcaaacgg gcagttgata tggaccccac cagcatgaag cgtaccgaga
    12961 gcatggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcagt
    13021 gaaatactgt gcgtggggtg agaggcgaac attcccggct ggcgaatcgc ctgattcgcg
    13081 ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt
    13141 aacccagctt caatcagtgc cggatagaga tttgctaccg ccgtgccgga ggtgacaatc
    13201 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc cagtccacgc
    13261 tcatcgaaat gggtgtggtg aatgaatgtg gaattctccg ccgccgctaa cgttaacggt
    13321 gtagaacgcg agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    13381 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    13441 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    13501 tgcgactcat tactattcca tttgtaataa agtacgcagc cctgccgctt tgttgtcgat
    13561 ttcctgccac tcttgctcgg ggtcggagcc acggacaatg cccgcgccag catatagccg
    13621 cacgacattg ccgctgattt ttgctgagcg cagggaaacg cagaattcgc tttgttgtaa
    13681 tgagagatag cccgcagaac cagcgtacca ttctcgggtg aacggttcgt gacgggcgat
    13741 aaactgtcgc gccagatcgc gcggtaaacc agcaactgct gccgttggct gcaactgatg
    13801 taaacagatc acatcatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    13861 tactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    13921 taatcgttgg cagatatctt ccaccaccaa catattctca cgctgatttt tatcatccgc
    13981 catcagccac tcacctaact gctgcgcctg cttatcatca ggatgatttg ctactgttcc
    14041 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    14101 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tgagattcag
    14161 tcgacgactg gccgccatca tcgccgccgc attgaccgga cttgcgaaat gcaggtcagt
    14221 tgcccgagca agcaccactt tgtcgagctc accttcggcg atggttttcg ttgccagttc
    14281 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtag tggttaaatg
    14341 taacccaggc aagggcttaa tactcaccag tgtggcgata aattctcttg ccttaatcgc
    14401 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    14461 gcgtcgccat tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcag
    14521 cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    14581 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    14641 ttgccagtag aattgcgggt acgtttgctg actggccagc cagctcaagg catcaaaagc
    14701 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    14761 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    14821 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    14881 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccaacggccg cacccacacc
    14941 aattccctgc cagggttttt cgtggacgta gtcatcagca cgataaacag cctgcttcgc
    15001 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    15061 ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    15121 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    15181 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagggt
    15241 tattgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    15301 atagatctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    15361 cgccccgagg tagacaggct tatcaggcca gtgatgcgtg caagtgtcga gggttttact
    15421 catcagttgc tgccccactt tttcgccacg caacgcttca ctgacaatca cccgacctat
    15481 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatacgcca ccagttcatc
    15541 atttttccaa ccgagaatat ggcgattatc ccctgtcaaa tcatcaccat cgatatcctg
    15601 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    15661 ctgggaaacc gataattcag agtgatgcag atcttgccat tcaatcataa tgctctcctt
    15721 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    15781 aattttttta ggtacttcgg ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    15841 gcttaacctg caacatccga cccagagcgg tctttggttg tttgactgcg gtgaaggcac
    15901 ccagcatcag ctactgcata ccgcctttaa ccctggaaaa ctggacaaga tttttatcag
    15961 tcaccttcat ggcgatcatc tctttggttt acccggcttg ctgtgcagtc gttctatgtc
    16021 tggtattgtc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggagac
    16081 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    16141 tggcgaaatt ctcgacgatg gcctgcgcaa agtgaccgct tatccaatgg aacacccact
    16201 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    16261 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    16321 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg ctccagtgcc
    16381 aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac ttgacctggc
    16441 taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg aagccaaagc
    16501 caatagtcgc ggtcatagct ctacacgcca ggctgcgaca ctggcccgtg aggctggagt
    16561 cggtaagcta atcattaccc acgtcagctc gcgctatgat gataaaggtt gtcagcacct
    16621 gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg gcgaatgatt tcaccgtgtt
    16681 taacgtttaa ctctgcttat tattaaaaac agggcgaaac ttgccctgtt atcgcaaccc
    16741 gcgctacata tatccaccca cacgggcata ttgagcgatt tttgatattt attcatctcc
    16801 ttacgcacta tattcagagc aataagttgc ctctttttat cttcattcag cactaccttt
    16861 gcaactaaaa ggagcatatg atggttacag ttgtcagcaa ttattgtcaa ttatctcaaa
    16921 cccaactcag tcagacattt gcagaaaaat ttactgtgac cgaggaatta ctacagtctt
    16981 taaaaaaaac agcgttatcc ggagatgaag aaagtattga gttactgcat aacattgcgt
    17041 ta
//
LOCUS       DAZFFG010000097        16866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFFG010000097 DAZFFG010000000
VERSION     DAZFFG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.097"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(166..2433)
                     /gene="adiA"
                     /locus_tag="ACHJ7J_003659"
     CDS             complement(166..2433)
                     /gene="adiA"
                     /locus_tag="ACHJ7J_003659"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO5001841.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2632..3540)
                     /gene="melR"
                     /locus_tag="ACHJ7J_003660"
     CDS             complement(2632..3540)
                     /gene="melR"
                     /locus_tag="ACHJ7J_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO5001842.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3823..5178
                     /gene="melA"
                     /locus_tag="ACHJ7J_003661"
     CDS             3823..5178
                     /gene="melA"
                     /locus_tag="ACHJ7J_003661"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO5001843.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5281..6702
                     /gene="melB"
                     /locus_tag="ACHJ7J_003662"
     CDS             5281..6702
                     /gene="melB"
                     /locus_tag="ACHJ7J_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO5001844.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6841..7470)
                     /gene="yjdF"
                     /locus_tag="ACHJ7J_003663"
     CDS             complement(6841..7470)
                     /gene="yjdF"
                     /locus_tag="ACHJ7J_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO5001845.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     MPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLKLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7593..9239)
                     /gene="fumB"
                     /locus_tag="ACHJ7J_003664"
     CDS             complement(7593..9239)
                     /gene="fumB"
                     /locus_tag="ACHJ7J_003664"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5001846.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9317..10657)
                     /gene="dcuB"
                     /locus_tag="ACHJ7J_003665"
     CDS             complement(9317..10657)
                     /gene="dcuB"
                     /locus_tag="ACHJ7J_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO5001847.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVNPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11228..11947)
                     /gene="dcuR"
                     /locus_tag="ACHJ7J_003666"
     CDS             complement(11228..11947)
                     /gene="dcuR"
                     /locus_tag="ACHJ7J_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO5001848.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11944..13575)
                     /gene="dcuS"
                     /locus_tag="ACHJ7J_003667"
     CDS             complement(11944..13575)
                     /gene="dcuS"
                     /locus_tag="ACHJ7J_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO5001849.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13756..13986
                     /gene="yjdI"
                     /locus_tag="ACHJ7J_003668"
     CDS             13756..13986
                     /gene="yjdI"
                     /locus_tag="ACHJ7J_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO5001850.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            13998..14270
                     /gene="yjdJ"
                     /locus_tag="ACHJ7J_003669"
     CDS             13998..14270
                     /gene="yjdJ"
                     /locus_tag="ACHJ7J_003669"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5001851.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            complement(14380..>16866)
                     /locus_tag="ACHJ7J_003670"
     CDS             complement(14380..>16866)
                     /locus_tag="ACHJ7J_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5001852.1"
                     /translation="CPVFSCAAVSPSPDNSHRFSFNPDFLQLSDGDNAKNIDLNYFAN
                     ASGAAPGEYTVDVVMNGELVDRQIKIDFSAQDNKLNARLTPQQLERWGIDTNKLSVSS
                     PSAYQQNIAYIIQGATEKFDANNQKLILNIPQSYLKPQDWLSTPPHLWDEGMPALMVN
                     YLFNGIKQNNNGYGSRSQFLSLDSSLNLGGWRLRHNGNWSTNSYNKESHWQPVSVYLQ
                     HDYSILQGGQFIVGQTSTDGAIFDSFPFEGAQFSSDDGMIAPELSQYSPVVRGIAYSQ
                     AQVSVKQNGVVIYQKNVPPGPFELRDFNQIFTGDLEVEIREADGTIRHFTQATAVLPV
                     LQRQGRLRYNLAFGKYRSGSTLNNSVSEPQFVQSSAAIGLPDEYTFYGGGIKADNYAA
                     VLLGLGKYSDFFGAFSLDVTHARSQFSQNYKSFGKQQGQSYRFMYSRGFGETNTTLNI
                     TGYRYATRGYYDFDELQQIQSGFVDEKNINSYHQRSRISTTINQDLQDWGQLYLSASK
                     DQYWDTADGYNLSASYSLPFRYISAMLSLGYNKSPYYHEADKSLFLSVSVPLNSLLNG
                     NNLFLTTNTITNNGQVQQQVGLNGSSQDGEFNYAVAQGWQNQNRGESGNINLNYRGPY
                     AQMNGGYAWQKDSSQWTYGISGGITLHPHGLTLSQPLSLDSASALVQARDAASVKVLN
                     GSGIYTDWRGYAVVPYLNPYNRNQISLDVNSVKDNVELLNTDVTVIPSRGALVSAPFK
                     VNVGNKAIITLIQKNGEPVPFGSVVTLDSENSINSSIVADQGQVYMSGLPEEDTLIAQ
                     WGDEPSQKCKTNYKLQNKTAKFSELTLQCQ"
BASE COUNT         4175 a         3983 c         4259 g         4449 t
ORIGIN      
        1 tttacagggt aaataacagg caaaaaaaag cgcggggtgg ggccgcgcaa aaagtatgac
       61 atttttgttt gcagcaattt ttatgaaaac gttgccatac tcaaaaggcc taaggccagg
      121 cggcgtaatg ttatttaaac aattacgcct tcagcggaat agtggttacg ctttcacaca
      181 cataacgtgg taaataccgt caataatttc agtcccttca gtttcgtgtt caaatccagg
      241 gaaatgatga tcccaggatt gcagcgagcg taaataactt acttgcggac tgtttttatc
      301 gccgaagttt tcaccagaca gcagcatcgg gattcctggc ggatacggga taactgagtt
      361 tgccgcgata cgtcctggca gattttcaat ggataccagt tcgacattgt tgtcgacaat
      421 cgcgttgtac gcttcacgcg gggtgatttc cgccaccggc aggccggaat aggcttcgtt
      481 caaccgtgcg ccagggttgt tttctttcag ccaggcaaac atagtgtcac ccagatcgtg
      541 aatccccatg ttcgcataag tgtcaggata ttgttcaaca agttccggca tcacctgcgc
      601 cagcggtgtg ttggcgtcat agtggcgttt gaaggagcaa agggtgttaa ccagagtccc
      661 ccatttccca cgggttacgc ccatagagaa caggaacata atttggaagt cagtggtgcg
      721 ggtaggcaca atgccgtggc gaccaagcca ggcagtgacc agcgccgccg gaacaccggt
      781 ttcttccagt tcaccatctt cacccattcc cggagcaagg atgctgactt taatcgggtc
      841 gagcatactc cagttatccg gaatatcttt gaagccgtgc cagctttcgc ccggatgcat
      901 tacccagcag tcctgaacgg tggtcagcag tttggttggt gcgtcagcaa agtcataggt
      961 tttgccggtt tgtgggtcgg tgacgacttc tttgttccac ggtttgaaga accagctacc
     1021 gtcagcggtg aactctttat acagccgcgc catcgcctga cggaaatcaa ccgcttcgtc
     1081 gatcacttcc tgcgtcagtg acaggccgct attgccgtcc atcatcgaca ccgccacgtc
     1141 gttggatgcg cagatggcat acagcgggga ggtggtggca tgcatcatgt aggcctggtt
     1201 gaagcgggag aagttaatcg ccccacgacc ttcacgtaca tgaatataag aggcctgtga
     1261 cagcgcattc agtagtttgt gggtggagtg ggtggcgaaa acggtaggac cgttgtgatc
     1321 gccaggttcg ccgcgcatgg catagtgatc ggcatagatc gggttgaaac gtgcatagcc
     1381 gtaccaggct tcgtcaaagt gcagacgatc ggaggttttt tccagcagat cctgcgcttc
     1441 tttagcgtta taacacacgc cgtcataggt gcagttggtc actacgcagt aagacggttt
     1501 ttgcccggct ttgtctttag tcagcgggct ttcactgatt ttcttctgca aggtttcagg
     1561 ttgcatttcc tgcggataga ttggcccgat aatgccgtag cggttgcggc ttggcaccat
     1621 gtagaccggt ttcgcgcctg tcagcatcaa gccttgttcg atggatttat ggcagttacg
     1681 gtcaacgacc acgacatcgt tatcggtcat gcaagcctgc atgatggtgc ggttagagcc
     1741 ggaagtaccg acgactaccg accaggagcg atcggcacca aatacgcgtg cggcatattt
     1801 ttcgctttcg ccaaatgcgc cagtatggtc aagcaaagaa ccgagggaag ttcgttcgat
     1861 gcccatgtcg gtgcggaaca gattttcacc atagtagtca tggtagaaac gtccggcggg
     1921 tgttttggta aaaccaacgc cgccctggtg gcctggcgct gcccaggaat attcatggat
     1981 gtcgctatat ttcatcagcg cgctgaacag tggcggcaac agctgctggc ggtagcgggt
     2041 catcgcggca acagcgcgtc cggcgataaa gtcggcggta tcttccagaa tccaggcgaa
     2101 ttcatcgaca agctccagca gatcccggtc cattgcggcg agggcttttt cccgatcgcc
     2161 caacaggaag accggcacgt tttgttggcg ctcatgaagc ttaccgatca attgtctgac
     2221 gttttgatgt tcgtccggat gttccatttg atagctgaac atcaggcagt caatggcttc
     2281 gtttgaagag agaatggcaa aaccatcatc aaaggaggtg gatttaatca cggtaacatt
     2341 ttgctggctt aaagcatctg ccagacgctc aacagcgtta ccgacccagg tgtcttgatg
     2401 gagaaactcg ctttcaacaa ttaatacttt catcattgct tacccggtta tgaaggaatc
     2461 ttcgttgcgg gcaagtatct tccggccttt tcgggctgta aagcgcgtga aaaacaaaca
     2521 tttttgaaat atttgttttt ataaacaata tgttatgtga ttattttaaa ttattttcac
     2581 tgcttattat cgacggctaa actatttttt tggctgatac tgatatcgtc tttagccggg
     2641 aaacgtctgg cggcgctgtt ggctaagttt gcggtattgt tgcggcgaca tgccgacata
     2701 tttgccgaac gtgctgtaaa aacgactact tgaacgaaag cctgccgtca gggcaatatc
     2761 gagaatactt ttatcggtat cgctcagtaa cgcgcgaacg tggttgatgc gcatcgcggt
     2821 aatgtactgt ttcatcgtca attgcatgac ccgctggaat atccccattg catagttggc
     2881 gttaagtttg acgtgctcag ccacatcgtt gatggtcagc gcctgatcat agttttcggc
     2941 aataaagccc agcatctggc taacataaaa ttgcgcatgg cgcgagacgc tgtttttgtg
     3001 tgtgcgcgag gttttattga ccagaatcgg ttcccagcca gagaggctaa atcgcttgag
     3061 catcaggcca atttcatcaa tggcgagctg gcgaatttgc tcattcggac tgttcaattc
     3121 ctgctgccag cggcgcactt caaacgggct aagttgctgt gtagccagtg atttgatcac
     3181 catgccgtga gtgacgtggt taatcaggtc cttatccagc ggccaggaga gaaacagatg
     3241 catcggcaga ttaaaaatcg ccatgctctg acaggttccg gtatctgtta gttggtgcgg
     3301 tgtacaggcc cagaacagcg tgatatgacc ctgattgata ttcacttttt cattgttgat
     3361 caggtattcc acatcgccat cgaaaggcac attcacttcg acctgaccat gccagtggct
     3421 ggtgggcatg atatgcggtg cgcgaaactc aatctccatc cgctggtatt ccgaatacag
     3481 cgacagcggg ctgcgggtct gtttttcgtc gctgctgcac ataaacgtat ctgtattcat
     3541 ggatggctct ctttcctgga atatcagaat tatggcagga gtgagggagg atgactgcga
     3601 gtgggagcac ggttttcacc ctcttctcag agaggcgagg ggagtgtccg agcatcatga
     3661 ggccgaaaac tctgcttttc aggtaaatta ttcccataaa ctcagattta ctgctgcttc
     3721 acgcaggatc tgagtttatg ggaatgctca acctggaagc cggaggtttt ctgcagattc
     3781 gcctgccatg atgaagttat tcaagcaagc caggagatct gcatgatgtc tgcacccaaa
     3841 attacattta tcggcgctgg ttcgacgatt ttcgttaaaa atattcttgg tgatgtgttc
     3901 catcgcgagg cgctgaaaac ggcgcatatt gccctgatgg acattgaccc cacccgcctg
     3961 gaagagtcgc atattgtggt gcgtaagctg atggattcag caggggccag cggcaaaatc
     4021 acctgccaca cccaacagaa agaagcctta gaggatgccg attttgtcgt ggtggcattt
     4081 cagattggcg gttatgaacc ttgcacggtg actgatttcg aggtctgtaa gcggcatggt
     4141 ctggaacaaa ccattgccga tacgttgggg ccgggcggta ttatgcgcgc gctacgtacc
     4201 attccgcatc tgtggcaaat ttgcgaggac atgacggaag tctgccccga tgccaccatg
     4261 ctcaactatg ttaacccaat ggcgatgaat acctgggcga tgtatgcccg ctatccgcat
     4321 atcaaacagg tcgggctgtg ccattcggtg cagggaacgg cggaagagct ggcgcgtgac
     4381 ctcaatatcg acccggctac gctgcgttac cgttgcgcag gtatcaacca tatggcgttt
     4441 tacctggagc tggagcgcaa aaccgccgac ggcagttacg tgaatctcta cccggaactg
     4501 ctggcggctt atgacgcagg gcaggcaccg aagccgaata ttcacggcaa tactcgctgc
     4561 cagaatattg tgcgctatga aatgttcaaa aagctgggct atttcgtcac ggaatcgtca
     4621 gaacattttg ctgagtacac accgtggttt attaagccag gtcgtgagga tttgattgag
     4681 cgttataaag taccgctgga tgagtacccg aaacgctgcg tcgagcagct ggcgaactgg
     4741 cataaagagc tggaggagta taaaaacgcc tcccggattg atattaaacc gtcacgggaa
     4801 tatgccagca caatcatgaa cgctatctgg acgggcgagc cgagtgtgat ttacggcaac
     4861 gtccgtaacg atggtttgat tgataacctg ccacaaggat gttgcgtgga agtggcctgt
     4921 ctggttgatg ctaatggcat tcagccgacc aaagtcggta cgctaccttc gcatctggcc
     4981 gccctgatgc aaaccaacat caacgtacag acgctgctga ctgaagctat tcttacggaa
     5041 aatcgcgacc gtgtttacca cgctgcgatg atggacccgc atactgccgc cgtgctgggc
     5101 attgatgaaa tatatgctct tgttgacgac ctgattgccg cccacggcga ctggctgcca
     5161 ggctggttgc accgttaaaa cgcgactaaa cgctactgcg ccgggggatt tattccggcg
     5221 cacacctctg acgataccaa taacagaagg cgggcgttgg taacagcgac ccgataccct
     5281 atgagcattt caatgactac aaaactcagt tatggatttg gagcgttcgg gaaggatttt
     5341 gcgatcggca ttgtgtatat gtacctcatg tattactaca ccgatgtcgt cgggctgtct
     5401 gtgggtttgg tcggtacttt gtttctggtg gcgaggatct gggatgctat taacgatccg
     5461 attatgggat ggattgtaaa tgctacgcga tcgcgatggg gtaagttcaa accctggatc
     5521 ctgatcggta cgttggcaaa ctctgtaatc ttatttctcc tctttagtgc gcatctgttt
     5581 gaaggtacta ctcagattgt ctttgtttgc gtgacctaca tcctctgggg catgacttac
     5641 accattatgg atattccctt ctggtcgctg gttccaacca tcacgctcga taaacgtgag
     5701 cgtgaacaac tggttcctta tccgcgtttt tttgccagtc tggcgggctt tgttacggca
     5761 ggtgtgacgc taccatttgt taattatgtc ggcggtggcg atcggggatt tggctttcag
     5821 atgttcacac tggtactgat cgcctttttt attgtttcaa ccatcatcac tctgcgcaat
     5881 gtgcatgaag tcttttcgtc agacaatcaa ccgtctgctg aaggaagcca tctgacactt
     5941 aaagccatcg ttgcgcttat ttataaaaac gatcagcttt catgcctctt gggtatggct
     6001 cttgcttata atgtagccag caacattatt accggctttg ctatctatta tttctcatat
     6061 gttatcggtg atgcggattt gttcccctat tatctgtcgt atgcgggagc tgctaacctg
     6121 gtgacgttag tattcttccc acgcttagtt aaatcattat cccgacgcat tttatgggcc
     6181 ggagcatcta ttcttccggt gttaagctgt ggtgttctcc tgttaatggc attaatgagc
     6241 tatcacaacg tcgtcctcat tgtgattgcg ggtattttgc tgaatgtggg aacggcgctt
     6301 ttctgggtat tacaggtcat catggtggca gataccgttg attacggtga atataaactg
     6361 cacgtacgct gtgaaagcat cgcttactcc gtgcagacta tggtggtgaa gggcggttca
     6421 gcctttgcgg cttttttcat tgcggttgtg ttagggatga ttggctatgt accgaatgtt
     6481 gaacagtcta cgcaagccct attaggtatg cagtttatta tgattgctct accgactctg
     6541 tttttcatgg taacgctgat tctctacttc cgtttctatc gcctcaatgg cgacacgctg
     6601 cgcaggatcc agattcatct gctggataaa tatcgcaaag taccgcccga gcctgttcat
     6661 gctgatattc cggtcggtgc agtgagtgat gtgaaagcct gacgtggaca atagctaacg
     6721 aaaatggcct gatgcgatat gtttatcagg ccacgtctta atgtaatgct ttggatttgc
     6781 aaattttgta gaccggataa ggaattcacg ccgcatccgg catcaacaaa gtgcacgttg
     6841 ttatccggtt atcaagccaa agcgccgtag ctggcggcaa tgaaagcgag cgaggaatat
     6901 caccgtcgtt aatgcgccaa gcagcgcgca gaacatatcg gattgcgtgt cccactggtc
     6961 gccctgggtg ccgagaaaat catccgctcc ctggcccatt gccagcgccg cccaccactc
     7021 gattaattca tacatcgcgc ttatcgccag cgccacgcaa cagaccagaa acgccaccat
     7081 tttacgtccg cgaacgtaca tcccgcgcac aaggatctca cgtgccacca gtgcaggcac
     7141 cagcccctgg aaaaaatgcc ccagcttgtc atacggatta cggctcaact tcagccattc
     7201 ctgcacctca aaaccaacgg ggactttcgc gtaggtgtat tgtccgccga ccatcaggat
     7261 gatggcgtga aagaaaatga gcgtataaag cagcggcgtt aacggataac gtttcgcggt
     7321 ggcaaggagt agcggcatga caataatcac cggtgtcact tccatcagcc aggtgagttt
     7381 gtcatgggcc gaaatgccgg tataaatcag gattaacgtt agcgccagtg cgccggtgtt
     7441 aagaattaac ggcttgagtg tgcgggtcat ggtgagttcg caagtcaggg aaaatactga
     7501 ctattcacca gcaagcgtga aattacaatc gcaaaagagg tggcccaggg ggatcacctg
     7561 gcagcatgct gccaggcgct gggccgaaga ggttacttag tgcagttcgc gcactgtttg
     7621 ttgacgattt gctggaagaa gtcgttacct ttgtcatcga ccaggataaa cgccgggaaa
     7681 tcttctactt cgattttcca gatagcttcc atacccagct ccggataagc gacgcattcc
     7741 agatgcttga tgctctgctg cgccagtacc gccgccggac cgccgatgct accgaggtag
     7801 aagccgccgt gtttatgaca cgcgtcggta acctgctgac tgcggttacc tttcgctagc
     7861 atgatcatgc tgccgccgtg ggattgcagc agatccacat aggagtccat acgacctgcg
     7921 gtggttgggc caagtgaacc tgatggataa ccggcagggg ttttcgccgg acccgcgtag
     7981 tagatcgggt gatctttgat gtactgcgga agttctttac cggcgtcaat cagctctttc
     8041 agcttggcgt gtgcaatatc tcggcccacg ataatggtgc cggtgagcga caaacgagtg
     8101 gaaaccggat attgcgaaag ctgggcgagg atctctttca tcgggcggtt aaggtcgacc
     8161 ttcaccgctt cgccttcacc ggcctggcgc agttcttgtg gaatgtactg acctggattg
     8221 tgttccagtt tttcgatcca gataccttcg cggttgattt tcgctttaat gttacggtca
     8281 gcggagcagg agacgcccat gcctaccggg caggatgcgc cgtgacgtgg cagacggata
     8341 acgcgaatgt cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc gagtttctgg
     8401 gcctcttcca gcagttcctg ttccagctgg acatcgcgga acgcctgacc atgttcgttc
     8461 ccttccgtcg gcagttcatc gtaatagtga gcgcttgcta acttaacggt tttcaggttg
     8521 gtttccgcag acgtaccgcc aatcacaaac gcgatatggt acggcgggca ggctgcagta
     8581 ccgagggtac gcattttctc gacgaggaag tttttcagtt tgccgggagt cagcagggct
     8641 ttggtttcct ggtagagata ggttttgttg gcagagccgc cgcctttcgc aacgcaaagg
     8701 aatttgtact catcgccatc taccgcgtac aggtcgattt gcgcaggcag gttagtgccg
     8761 gtgttgacct ctttgtacat gtccagcgcc gcgttttgcg agtagcgcag gttatcttcg
     8821 atataggtgt tatagacgcc tttcgacagc gcttcttcat caccgccacc ggtccacacg
     8881 cgctggcctt ttttaccgac gatgatcgcg gtgccggtat cctggcaggt cggcagcacg
     8941 cctttggcgg cgatttcgga gtttcttaag aattgcagtg ccacgtactt gtcgttttcg
     9001 ctggcttctg gatcgtgaag aatagccgca acctgtttct ggtgtgccgg acggagcatg
     9061 aaagaagcgt cgtgaaaggc ttgctgcgcc agcagggtca gggcttctgg ttccactttc
     9121 aggatggttt cgccgtcgaa gtcggcaacg ctaacgtaat cggaagtgag tagatagtat
     9181 tcggtattgt ctttccccat cgggaaaggt gcctggtaga taaagggttt gtttgacata
     9241 gcttccagcc tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc
     9301 ggcacgcaaa gtgcatttat aagaacccgt acatcgcggc gaagatccag ccgaagacgc
     9361 acgatacgct cacaccaatc aaccccggca gaataaagct gtggttgatg acgaagcgac
     9421 cgatgtgggt ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc ggataagtcg
     9481 gcaggatgta ataaccgtag caagccggtg ctgaagccac gatgtatgcc ggattaacgc
     9541 cgatcgctag cgcgaccgga acaatcgccg ccagcgccgc agcctgagag tttacaaact
     9601 tggaaaccag cagcagaaca atggcatagg cccacggata ctctttcacc atttcaccca
     9661 gtacgccctg aatttcagac atatgcgcac cgaacatggt ttctgccatc catgcgatac
     9721 cgtacaccgc cacgatggcg atcataccgg aacggaagac ttcgtttttt gagatagacg
     9781 cgggattggt tttggtcagg ataataatca gcgccccggt cagcagcata aacatctgaa
     9841 taaccagtac catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac
     9901 caagaagtgc gactacagcg attgcaccga ggaaaatcca cattgccagc cagttgcttt
     9961 tcggcagttt tttatcgagc agcgtcgcgg tatcaccgta aacatactca cggttttccg
    10021 gtactgagat gaatttctgg aactcttcgt ctttatccag atctttaccg cggaaccagc
    10081 tgaagatacc gatcgccagg ataccgatta acgtcgatgg aatggtgatt gccagcaggt
    10141 cgaggaactc aagatggcga ccatcaaagg tgacattacc cagcattgca accagagaca
    10201 cgaccgcaac cgacaccgga ctggcgataa tccccatctg tgcaccgata gaacttgccg
    10261 ccatcggacg ttccggacgg atgttgttct taatggcgac gtcgtagatg atcggcagaa
    10321 tggtgtaaac cacatgaccc gtaccgcaaa gaatggtcag tgtacaggtc acaaacggcg
    10381 cgacaattga gacatatttc gggttgcggc gcagcagctt ctcggcaatt tgcagcatga
    10441 catcaagacc gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca atgataacca
    10501 gcatgacatc aaccggtggt ttacctggct gaaggtggaa gacgaagacc agaatgacca
    10561 gaccgatacc gcctaataaa cccagcgcga taccaccctt tctggcacca taaaacagac
    10621 atatcagtat tatgataagt tggatagtaa ataacatgtg tgaaccctcg cgataatcct
    10681 atttaaattt ttgctgaata gatcacagtc acgttctgtt ttgtatgaac tgttttcaga
    10741 gcctgactaa atatccgttt ggtcgttacc ggctttagca aatacctcac agtgaatatt
    10801 ggctgattaa agcagaggca aatttatgca ctgtgataag cggctttttc agagagaaaa
    10861 actctctggc gatgggttta aagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt
    10921 atcttattga tatttatcgg tctatttttt gttctcaggg cagatatcat taggtttata
    10981 gattaatctg atctacccat ttgtgggtaa aaatacacat aatgcgggtg acataatagt
    11041 taattaactt ttgttagcgt tttgaaatta aaaacaccgt tcacctgaag agatattaat
    11101 ttttagcgat gatggaggga taattatatt tgatctggca caagttttac tgatgaagga
    11161 tgtaacttgt gccaggggta ttttgcatta cggtaataat tattacttac agataaccag
    11221 cgatccgtta ttggcaatat tgtttcagta gtgagtagtg ttctgcctga atacggtaac
    11281 ggtaaactgg acgccctgtg acgccataat ggatactggt gaataagatg tggcagttga
    11341 ccagccagat gaggtattta cggcaggaaa cacgcgaaat gttaacctcg ttggctagct
    11401 cgtcggttga aaactcatag tcctgatgcg cgtcaatcca ctggcacagt gtgcgtaacg
    11461 tctgcggcgt taagcctttt ggcaagcgac gaggatcctg ctcgttggag ctgctgccgt
    11521 ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct
    11581 tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt
    11641 aatccacgac accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga
    11701 tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt
    11761 tctctttttg catatagata tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga
    11821 taatctcttt ggctttctcc agcgtcgagg ctgttccaca gcattgaaag cctgggattt
    11881 gtgctacgta tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat
    11941 tgatcatctg ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat
    12001 cccgggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag
    12061 tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc
    12121 gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcgcagtgca gccagccgtg
    12181 acggtagtgc aatgttacgc taatttcgcc accgggttcc ggccctaatg cttccagcgc
    12241 gttttctatc agatttccca atgtggtaat cagcgtcgcg acctggtcct cactgccgct
    12301 gtctggcagc tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt
    12361 aatcttgctg attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc
    12421 aatctcttcc tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact
    12481 cttcagatgc aataatccga gaatcacatg caatttattc ataaattcgt gggatcgttc
    12541 acgaagtgcg tcagcatagt tgaccagacc atcgagtcgc tgcatcagtt tacgtacttc
    12601 agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac
    12661 ggtgttgatc agtaatagcc gatctttaat cgtaatctct tcgtcgcggc gcggggtacc
    12721 gtcgcgtaac acttccgaga catctaccac ctgtgaccac gagtggctta gcgtcgacag
    12781 tttctcatcg tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag
    12841 cgtgacctcg ccgcgatcgt ccacggcaac gacgccttct ttgatagact gcaacatggc
    12901 ctggcgttgc tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggattttttt
    12961 cagtacctta accagaatgc aggtgccaat cagtccgacc agcatgccaa ataataccga
    13021 ccagataatg ctccagcgac tgtcattgat ctgttgggtc acgcggctta actcaaggcc
    13081 gatcgccacc acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa
    13141 agcctgcgcc agaaaaccgc gattgatagc gacattttct tcgccattca gcgctttaag
    13201 gatgtcatca cctttaaatg gctgaccaat acgctgggct tcaggatgcg agtagcgaag
    13261 actttgcata tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc
    13321 gatggcctgg attccgctct cctgcggttt tttctgcaag ccctgacgga tttccggcga
    13381 gtcggcgaga gtacgcgcca ctgccagtgc cttgttggct agcccgtctc gcgtcatatc
    13441 actgatttgc gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcgct
    13501 gaccattaag atcactgtgg tactcaattt catcggacgt ttgcgtaaca tgcggtaggg
    13561 caatgaatgt ctcatcagct tccttgtgtg acaaatttct taagcattat ctctgatgag
    13621 gcgggtaatt caaagggagt aagaatgatt ggctatatag gggaagagac tctggcaacg
    13681 gaaactgcca gtgctgtatg aggattccag ggctatgctt atagtgaaaa ccatactgat
    13741 gagagaggga aggtcatgga tcaggcgcta ctggacgggg gttatcgctg ttataccggc
    13801 gaaaagatcg atgtctattt caacactgcg atatgtcagc attctggcaa ttgcgtacgt
    13861 ggcaacggca agttatttaa tctcaaacga aagccgtgga tcatgccgga tgaagtcgac
    13921 gttgccactg tggtgaaagt gattgatacg tgcccgagcg gcgcgctgaa ataccgtcat
    13981 aaataagcga gggtaaaatg gaaatacgcg aaggccacaa taaattttac attaatgacg
    14041 aacagggcaa gcaaatcgct gaaattgtct ttgtgccgac cggagagaat ttagcaatta
    14101 tcgaacatac cgatgtcgat gaaagcctga aagggcaagg gatcggtaaa cagctggttg
    14161 cgaaagtcgt ggaaaaaatg cgtcgggaaa aacgaaaaat tatcccacta tgcccatttg
    14221 cgaaacacga atttgataaa acgcgggagt atgatgatat tcgcagttga aaagatgatt
    14281 tgtatccgcc ggaagtaata caaatctgga atgatgttgt taatatgtta gatgaaaata
    14341 accctgcata tgcagggtta ttaatcttag aactagcgac tattggcatt gtaaagttaa
    14401 ttcactgaat ttcgcagttt tattttgcaa tttgtaattt gttttacact tttgagaagg
    14461 ctcatcaccc cattgtgcaa taagtgtatc ctcttcaggt aaccctgaca tatacacctg
    14521 tccttgatca gcaacaatac tgctgttaat gctgttttca gaatctaatg tcaccacact
    14581 accaaacggt acgggctcac cattcttctg tatcaaggta atgattgcct tattaccgac
    14641 attgacttta aatggagcgg aaactaaagc tcctcgagaa gggatgacag taacatctgt
    14701 atttagcagc tcaacattat ccttaacact attaacatca agtgaaattt gatttcggtt
    14761 atagggattc aaatatggga ccacggcata tccacgccag tccgtataaa tacctgaacc
    14821 gttcaatact tttacgctgg cggcatcacg agcctgaacc agagcactgg cactgtccag
    14881 acttaatggc tgggataagg ttaatccatg tggatgcaac gtaataccac cgctaattcc
    14941 gtatgtccat tgactggagt ctttttgcca ggcatagccg ccgttcattt gtgcataagg
    15001 tccacgatag ttaaggttaa tgttacctga ctctcctctg ttttgattct gccagccttg
    15061 cgccacggca tagttaaact caccatcctg tgaagagccg tttaatccaa cctgctgttg
    15121 cacctgacca ttattcgtaa tggtattcgt ggttaagaac aggttatttc cattcaacaa
    15181 tgagttgagt ggtacagaaa cagataaaaa cagcgactta tcagcctcgt ggtagtacgg
    15241 acttttatta tacccgagcg atagcattgc tgagatataa cgaaatggca gtgaataact
    15301 cgcagataag ttatatccat cagcggtatc ccagtactga tctttacttg ctgagaggta
    15361 gagttgtccc cagtcctgaa gatcttgatt tatcgttgtt gatatacgcg aacgttgatg
    15421 ataactgttt atattttttt catcaacgaa tccactctga atttgttgta actcatcaaa
    15481 atcgtagtaa ccgcgtgtgg cataacgata gccggtaata ttcaatgtcg tatttgtctc
    15541 accaaaacca cgcgaataca taaaacggta agactgtcct tgctgttttc cgaaagactt
    15601 ataattctgc gaaaattgtg agcgagcatg agtgacatcc agggagaacg ctccaaaaaa
    15661 atctgagtat ttacccaggc caagcagaac tgctgcatag ttgtctgctt tgataccacc
    15721 accataaaat gtatattcat caggtaatcc aattgctgct gaagactgaa caaactgagg
    15781 ctcagacaca ctattattca aagtagaacc tgagcgatat ttcccgaatg ccaggttata
    15841 gcgtaagcgt ccttgacgtt gaagaaccgg taaaacggca gtcgcctgag taaaatggcg
    15901 aatagtgcca tctgcctcac ggatttccac ctccaggtca ccggtgaata tttggttaaa
    15961 atcgcgtaat tcaaacggtc caggtgggac gttcttttga taaatgacaa caccgttttg
    16021 cttgacggat acctgtgctt gagaataagc aatgccacgg acaacagggc tatattggct
    16081 gagttctggg gcaatcatgc catcatcaga tgaaaactgt gctccttcaa aaggaaaact
    16141 gtcaaatatc gcaccatccg ttgaagtctg acctacgata aattgccctc cttgcaggat
    16201 agaataatca tgctgtagat aaacactaac gggttgccag tggctctctt tattatatga
    16261 gtttgttgac cagttaccgt tatggcgaag gcgccatcca ccaaggttca gagaactgtc
    16321 cagagataaa aactgactac ggctaccgta gccattatta ttttgcttaa ttccgttaaa
    16381 tagataatta accattagcg ctggcatacc ttcatcccag agatggggcg gagtacttaa
    16441 ccaatcctga ggtttgaggt agctttgtgg tatattcaat attagctttt ggttgttcgc
    16501 atcgaatttc tctgttgcgc cctgaataat atatgcaata ttttgctggt aagccgatgg
    16561 ggaagataca gagagcttat ttgtatcaat tccccaacgt tccaactgtt gaggcgtcag
    16621 tctggcgttc agcttgttgt cctgcgcgga aaaatctatc ttaatctgac gatcaactaa
    16681 ttccccattc ataactacat caacagtata ttcccccgga gctgcaccgc ttgcgtttgc
    16741 aaaataattg agatcgatat ttttggcatt atcaccatca gataattgca aaaaatcggg
    16801 gttaaatgag aacctatgac tattgtcagg tgagggagat acggcagcgc aactgaatac
    16861 cggaca
//
LOCUS       DAZFFG010000098        16704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFFG010000098 DAZFFG010000000
VERSION     DAZFFG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.098"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(117..269)
                     /locus_tag="ACHJ7J_003671"
     CDS             complement(117..269)
                     /locus_tag="ACHJ7J_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5001853.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(534..1268)
                     /gene="cysH"
                     /locus_tag="ACHJ7J_003672"
     CDS             complement(534..1268)
                     /gene="cysH"
                     /locus_tag="ACHJ7J_003672"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5001854.1"
                     /translation="MPKLDLNALNELPKVDRILALAETNAELEKLDAEGRVVWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1342..3054)
                     /gene="cysI"
                     /locus_tag="ACHJ7J_003673"
     CDS             complement(1342..3054)
                     /gene="cysI"
                     /locus_tag="ACHJ7J_003673"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO5001855.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIERIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3054..4853)
                     /gene="cysJ"
                     /locus_tag="ACHJ7J_003674"
     CDS             complement(3054..4853)
                     /gene="cysJ"
                     /locus_tag="ACHJ7J_003674"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO5001856.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQTAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAAATPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRTRAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5172..5534
                     /gene="queD"
                     /locus_tag="ACHJ7J_003675"
     CDS             5172..5534
                     /gene="queD"
                     /locus_tag="ACHJ7J_003675"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO5001857.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5612..6883
                     /gene="ygcN"
                     /locus_tag="ACHJ7J_003676"
     CDS             5612..6883
                     /gene="ygcN"
                     /locus_tag="ACHJ7J_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5001858.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLENGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6874..7134
                     /gene="ygcO"
                     /locus_tag="ACHJ7J_003677"
     CDS             6874..7134
                     /gene="ygcO"
                     /locus_tag="ACHJ7J_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5001859.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7151..7726
                     /gene="ygcP"
                     /locus_tag="ACHJ7J_003678"
     CDS             7151..7726
                     /gene="ygcP"
                     /locus_tag="ACHJ7J_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO5001860.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINSGVVALSTTNTGVWTLAKKLL"
     gene            complement(7874..8734)
                     /gene="ygcQ"
                     /locus_tag="ACHJ7J_003679"
     CDS             complement(7874..8734)
                     /gene="ygcQ"
                     /locus_tag="ACHJ7J_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5001861.1"
                     /translation="MNIAIVTINQENAALAGWLAAQDFSGCTLAHWQIEPQPVVVEQV
                     LDALVEQWQRTPANVVLFSPGTFGDELSTRLAWRLHGASICQVTSLEIPIVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLVSIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIDAECQ"
     gene            complement(8731..9510)
                     /gene="ygcR"
                     /locus_tag="ACHJ7J_003680"
     CDS             complement(8731..9510)
                     /gene="ygcR"
                     /locus_tag="ACHJ7J_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO5001862.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9488..10897)
                     /gene="ygcS"
                     /locus_tag="ACHJ7J_003681"
     CDS             complement(9488..10897)
                     /gene="ygcS"
                     /locus_tag="ACHJ7J_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5001863.1"
                     /translation="MMGRYLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAAMPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLILAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10919..12373)
                     /gene="ygcU"
                     /locus_tag="ACHJ7J_003682"
     CDS             complement(10919..12373)
                     /gene="ygcU"
                     /locus_tag="ACHJ7J_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO5001864.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIIVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12443..13228)
                     /gene="ygcW"
                     /locus_tag="ACHJ7J_003683"
     CDS             complement(12443..13228)
                     /gene="ygcW"
                     /locus_tag="ACHJ7J_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5001865.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13547..14824
                     /gene="yqcE"
                     /locus_tag="ACHJ7J_003684"
     CDS             13547..14824
                     /gene="yqcE"
                     /locus_tag="ACHJ7J_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO5001866.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14851..16329
                     /gene="ygcE"
                     /locus_tag="ACHJ7J_003685"
     CDS             14851..16329
                     /gene="ygcE"
                     /locus_tag="ACHJ7J_003685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO5001867.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIIGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEPTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPETLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYTWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3999 a         4396 c         4176 g         4133 t
ORIGIN      
        1 aattcataat aataaataga gactatatat tcacataaaa aatataaatt ttaaaaaggg
       61 tgcaggagac caaaccatca caattcagta cgcggggttt tacccccgcg tggtggctac
      121 ttcttcgatt cgtaagcgag aactgcctta aactccatac cacgttctct gatactcaac
      181 tcacacaacg agtcgcctac catcagcgtg aatcctagta ctgtgcaaca cagtacgatg
      241 attgccacaa gggcatattt tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca
      301 ttcgaattgt ttgggttcga acgtaagcct caggttgata gaaatatcgc ctggggcttt
      361 tgtccatctg gaacctcgcg aatgcttaac gccagacagc ctcaagcacc cgacgccatt
      421 ctatacctga taattcttcc agcggcgttc acgtttaaaa tcaggaatgc tgctcgcaaa
      481 ataaaaacgc ccccatcatt tctgacagag gcgtttaatt tatccggcaa tatttaccct
      541 tcgtgtaacc cacattccct ttttaagcca aagaaacgcg tttcttcttc cgccatgccg
      601 ggttcccatt tacgggttgt atgggtatct ccaaccgata aatatccttc atcccataat
      661 gggtgatatt tcaggccatg tttttgcagg tactgatata tcgtccgatt atcccagtca
      721 ataatgggca gcaccttaaa tacgccacgc tgaattgcca gcaccggtaa attggcgcgg
      781 ctgccggatt gttcgcggcg caggccagca aaccacgttt gcgcgttaag ttctttcagc
      841 gcccggttca tcggttcgac tttgttgatg tcattgtact tttcaatgcc ttcaacgccc
      901 tgttcccaca gttttccgta gcgtgcttcc tgccaggctg cgctttcggt agcacggtac
      961 actttcaggt tgagcttgag tttgtccgtt aactcgtcaa taaagcggta ggtttccggg
     1021 aacaagtaac cggtatcggt gaggatcact ggaatatccg ggcgaatctg attcaccaga
     1081 tgcaggctca ccgccgcctg aatgccgaag ctggaagaaa gcacatattc accgggcaga
     1141 ttatccagcg cccagactac gcggccttca gcgtccagtt tctccagttc ggcgttagtt
     1201 tctgccagcg ccagaatgcg atctaccttc ggcagttcgt tcagggcgtt tagatcgagt
     1261 ttgggcatag attcctcact gtttgccttg cctgatgcga cgctcacgcg tcttatcagg
     1321 cctacaagac cgggctgatg gttaatccca caaatcgcgc gccggatcga gcaccgggcg
     1381 aatgatgccc gcacgcaccg taaagtcgcc gaagccttca cccgcttcgc gctctttcgc
     1441 ccagcgccct atcagttcat caagcgacgc caggatttcc ggctcggtga tgttttcttt
     1501 atacatccgt gggatacgtg tcccaatacg gttgccgcca agatgcaggt tgtagcgacc
     1561 cggcgcttta cccaccaggc ccacttccgc cagcatcgcg cgaccacaac cgttcgggca
     1621 gcctgttaca cgcatcacga tatgctcatc gctaacacca tgtttcgcca ttaaattatc
     1681 gatgttgtcg ataaaagacg gtaggaaacg ctccgcttcc gccatcgcca gcgggcaagt
     1741 cgggaacgag acgcaggcca ttgagttttc acgctgcggc gtgacggcat tcattaaccc
     1801 gctctctttg gcgatcctct caatcttcgc tttctcactt tccggcacac cggcgatgat
     1861 cagattttgg ttcgccgtaa tgcggaaatc acctttgtgg atctttgcga tctccagcag
     1921 gccggttttc agcggacgcc caggataatc aaggatgcga ccattttcga taaacagcgt
     1981 caggtgccag ttatcatcaa tgcccttaac ccagccaata cgatctccgc gtccggtgaa
     2041 ctcatacgga cggatcggtt caaatttgat ccccgcgcga cgctccactt ccgctttaaa
     2101 cgtctcaacc cccacgcgct ccagcgtgta tttggttttg gcatttttac gatcggttcg
     2161 gttaccccag tcacgctgag ttgtcacgac agcttccgcc accgccagcg tatgctccag
     2221 cggcagatag ccaaactcgc ttgcggtgcg ggcgtaggtt ttcttgttgc cgtgttcgat
     2281 agaaagccca ccgcccacca acaggttaaa gcccaccagc ttgccgtttt cggcgattgc
     2341 cacgaagttc atgtcgttgg catgcagatc gatatcgttc tgtggcggga tcactaccgt
     2401 ggttttgaat ttacgcggca aataagtctg accgaggatc ggttcttcat cggtggtggc
     2461 gactttttcc tgatcgagcc agatctccgc atacgcgcgg gtacgcggca gcagatgttc
     2521 agagattttc ttcgcccatt catacgcttc tgcgtgcagc tgcgactcgt aagggttcga
     2581 ggtgcagagt acgttacggt tcatgtcgtt agcggtcgcc agcgcatcaa gaccgaccga
     2641 gtgcagcatc tggtgcaccg gtttgacgtt ctttttcaga atgccgtgga actggaatgt
     2701 ctggcggttg gtcaggcgaa tgctgccata gatggtgttt tcaccggcaa atttatcgat
     2761 cgcctgccac tgtttggtgg tgataactcc acccggcaga cggcagcgca acagcatcgc
     2821 atggcgcggc tccagcttct gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg
     2881 atacatgccg tggaagcgga tcagcaggaa gttatcgccc ttaaagccgc cggtcaggcc
     2941 gtcgtttaaa tcttccgcaa tggtgccgcg caggtagttg ctttcaagct tcatgcgctc
     3001 ggcgtctgtc agttttcctt cgaccactaa aggccctgga tgtttttcgc tcattagtag
     3061 acatctcgct gataacggcg ctctacgcgc agctcactta aaaattcatc cgccgcttcg
     3121 gtgtccatgc caccaaattc ggcaattact tccagaagtg cctgctcaac gtctttcgcc
     3181 atgcgattag cgtcgccgca gacataaatg tgggcaccgt cattgatcca gcgccacagc
     3241 tccgcgccct gttcgcgcag tttgtcttgt acgtaaactt tttctttttg atcgcgcgac
     3301 caggcgagat cgatccgcgt cagcacaccc tctttgacgt agcgctgcca ctccacctga
     3361 tacaggaagt cttccgtaaa atgcggattg ccaaagaaca gccagttttt acccggtgct
     3421 tcgtcagcgg cgcgttgctg cataaaggcg cggaacggcg cgatgccggt gcctggacca
     3481 atcataatca ccggggtttc cgggttagcg ggcaggcgga agttatcgtt atgttcgata
     3541 aatacgcgga cttcgccctc ttcttccacg cggtcagcga ggaagctgga ggcaccaccg
     3601 gcacgggtgc ggccttccac gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc
     3661 tcgacttccg cctgcgagga ggcgatggaa tacagacgcg gcgtcagcgg gcgcagcaga
     3721 ttaatcagcg cttcggcgtc cagttgcgcc ggagagaagc gcaccatgtc gacaatcggc
     3781 gtggccgcgg cgtaatgctg caacttcgct ttatcgccca ccagcggcag cagtgtttcg
     3841 ctgcgggtaa gcgtggcgta attctcaaca atgttggcgg tgttgacggt cagttcgaag
     3901 tgccactgta gcgcttcgtt cagaggcaac gttttgccgt caacggtgac aggttcatcg
     3961 cctttcagcc acagcagttc gacaagttct ttcaccagtg ccggatcgtt ctgataccag
     4021 acacccagcg cgtcacccgg ctggtaacgc aggcccgaat cacctaagtc aatttcgata
     4081 tggcgaacgt ctttttcaga gttacgcccg gtaattttct ggttaaccga aaggctagcc
     4141 accagcggcg cgtctttgct gtacgggctg gtgtggattt catttaccgc gccagtagcg
     4201 acggattgcg aaggtgccgc gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg
     4261 cgccactcgc tggcagcggt ctggtattca acgtcggcgt cgacacggtc gagcaggcgt
     4321 tcaccgccca gttccgccag cttgctgtcg aaatctttcc cggactggca gaaaaattca
     4381 taagaactat cgccgaggct aaacacggca aaagcggtat tttccagctt cggcgctttt
     4441 ttggagaaca ggaacttatg cagcgcgacg gcttcttccg gcggttcccc ttctccttgc
     4501 gttgacgtca ccacgataag cagtttttcg ctggcgattt gtttgaattt atagtcgccc
     4561 gcgttcacca gcttaacgtt cagttttgcc gtcaataaat catcgcgtaa tgcttcagca
     4621 acccggcgcg cattgccggt ttgcgaggcg gagataatag ttatacccgg catttctgcg
     4681 gctggcgctg gcgtcgctgc aagcgcagca ggctgctgat tgagcacgcc ccagaaatag
     4741 ccagaaaccc aggcaagctg ggtgggagtt aaatcggtcg tggccgcctg aaggcgtgcc
     4801 agttgctccg ggttcaacgg aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt
     4861 tatgttccag taagcaaagc tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa
     4921 ggtaaggtta acggggtgaa cggtgtggat taaagacggg atagcgataa ctaataacca
     4981 aatcgactaa cctgttttag caatagtctt taacaacaaa atagattaac caacctaatg
     5041 aaaaacaaat gaatttagcc aatcattaag ataaatcagc gattttgcgc aacaaagtcg
     5101 ttttagataa tgcgaaaaaa cagcctttcc ggtactctac ggcggtttta tcgtcctgta
     5161 gagaaattat gatgtccacc acgttattta aagatttcac cttcgaagcc gctcaccgct
     5221 taccacacgt cccggaaggg cataaatgtg gtcgcctgca cgggcattcc tttatggtgc
     5281 gactggaaat taccggggaa gtcgatccgc atacgggctg gattatcgat ttcgctgaac
     5341 tgaaagcggc gtttaagcca acttatgagc gcctcgatca ccattatctc aatgatattc
     5401 caggtctgga aaacccgacc agcgaagttt tagcaaaatg gatttgggat caggttaaac
     5461 ccgttgtgcc gctgttaagt gcggtgatgg taaaagaaac ctgcaccgct ggttgtatct
     5521 atcgcggcga atgataagag tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag
     5581 ggtgttgtgt tctggatatc tggggcatga catggaagac gactgcgaca ttattattat
     5641 tggtgccggt attgcaggca ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt
     5701 tttgttactg gaacgagctg aaatccccgg cagcaaaaat ctttccggcg ggcggttata
     5761 tacccatgca ctcgcggaac tcctcccgca atttcatctg accgcgcctc ttgaacgacg
     5821 catcactcac gaaagccttt ccctgttaac gccggatggc gcaacgacgt tttccagctt
     5881 acagcccggc ggtgaatcct ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc
     5941 cgaagccgaa aaagaaggtg tcgaatgcat ccctggtgcg acggtggatg cactgtatga
     6001 agaaaacggc agggtgtgtg gtgtcatttg tggtgacgat attctccgcg cccgttatgt
     6061 ggtgctggca gaaggtgcca acagcgtcct ggctgaacgt cacggattag tgactcgtcc
     6121 tgctggcgaa gcgatggcgt tggggatcaa agaagtgctg tcgctggaaa catccgctat
     6181 tgaagaacgt tttcatctgg agaataacga aggcgcagcg ttgctgttca gcggcgggat
     6241 ctgtgatgac ttacccggcg gcgcatttct ttatactaat caacaaacac tctcgttagg
     6301 gattgtttgc ccgctctctt cccttacgca aagtcgtgtt ccggcaagcg agctgctgac
     6361 tcgctttaaa gcgcatccgg cagtgcgccc gcttatcaaa aacacggaat cactggagaa
     6421 tggtgcacat ctggtgccag aaggtggctt gcacagtatg ccggtacaat acgccggtaa
     6481 cggctggctg ctggtgggcg atgcgttacg cagttgcgtc aataccggaa tttccgtgcg
     6541 cggcatggat acggcgctaa ctggcgcgca ggcggcggca caaacgctga taagcgcctg
     6601 ccagcaccgc gagccgcaaa atttgtttcc gctttatcat cacaacgtag agcgcagcct
     6661 gctgtgggat gttctacaac gttatcagca tgttccggcg cttttgcaac gccctggctg
     6721 gtatcgggcg tggcctgcgt taatgcagga tatttcccgc gatttatggg atcagggtga
     6781 taaacctgtt ccaccgctgc gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg
     6841 gcatctggcg ggcgatgtta tcaggagtct gcgatgtctg tagcccgtaa tctctggcgc
     6901 gttgctgatg cgccgcacat tgttccggct gactccgttg agcgccagac ggcagaacgg
     6961 ttgattaacg cctgtccggc aggtcttttt tcgctcacac cggaaggtga cttacgtatt
     7021 gactatcgca gttgcctgga gtgtggcacc tgccgtttgc tgtgcgacga atcaacacta
     7081 caacagtggc gctatccgcc ttccggattc ggcatcacct accgttttgg ataaataata
     7141 aggataattt atgcccctct tacacctgct ccgccagaat ccggtgattg ccgccgttaa
     7201 agataatgcc agcctgcaac tggctatcga ttctgaatgc cagtttattt ccgtgttgta
     7261 cggcaatatc tgcaccatca gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc
     7321 ttttattcat gtcgatttgc tggaaggcgc gtcaaataaa gaagtcgtta ttcagttttt
     7381 gaaactggtg accgaggcgg acggcattat cagtaccaaa gcctcaatgt tgaaagcggc
     7441 aagagcggaa ggttttttct gtattcatcg cctgtttatt gttgattcaa tttcgtttca
     7501 caacattgat aagcaagttg cgcaatcgaa tccggattgt attgagatcc tgccaggctg
     7561 catgcccaaa gtgctgggct gggtgacgga aaaaatccgc caaccgctga ttgctggcgg
     7621 gctggtatgc gatgaagaag atgcgcgtaa tgcgattaac tcgggtgtcg tggcgctttc
     7681 caccacgaat accggagtct ggacgttagc gaaaaaattg ctttgacggg ataatcgtaa
     7741 ccaattgaat ttggtttgat ttgtaggccg cacgccacat ccgacattca gcgcctgatg
     7801 cgacgcttaa cacgtcttat caggcctaca ggtccccagt ccagaaccgt aggcaggatc
     7861 atccggcagc acgttattgg cattctgcgt caatgtttgt caccagtgcc tccagcacca
     7921 ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa taccgctgcg ctggcgtcat
     7981 gattaatcgc caccacaaac ttgctattgc gtacgcccgc cattaacgct gctgcgccag
     8041 aagcgccaac aacaatacac acctcaggtg ccagcagatg tccggaaata ccaatcactt
     8101 tttcagcatc aacgccaccg ttcatcactc gcgcccgact gtagcccact tcggctccca
     8161 gcttttctgc cagcatagcg atttcctggt tatctgcctc tccgccctgc cccaccacca
     8221 gcacccgtct ggcctcagct aatgggtcgc gagtaacatt tttcaggttc tctatgctaa
     8281 ccagccagtc cggaagcgca cctgggacta tgttcagttg ctgcatacca gagggcaacg
     8341 tggcattttt atctgctccc gcctggcgcg ccagggaaag acatagcggg cgtttttctg
     8401 tttgcagcgt cgccgttagc gcatttcccc agtgggattt gcgcacgctt acgattggaa
     8461 tctctaacga agtcacctgg cagatgcttg caccatgtaa acgccaggcc agccgggtcg
     8521 acagttcatc gccaaacgtt cccggcgaaa agagaacaac gttcgcaggc gttcgctgcc
     8581 actgttccac cagcgcatcc agcacctgtt ccaccactac gggctgtggt tctatttgcc
     8641 agtgcgccag ggtacaaccg ctgaaatcct gcgccgccag ccagcccgct aatgcggcat
     8701 tctcctgatt tattgtcaca attgcgatat tcatggctgc atcctttggc gtaaatagtc
     8761 ctgccatagc ttttgcgctt tttctgctac cgtttgccca tcaatcagcg tcgctccgcg
     8821 tcgttgctct gctcttgcca gttgcagaca ctgcatcgct ggcaattcgg cagcaacagt
     8881 tttacggata atttccgctt tccccgcagc cattcgctgg cgcattcccg gtactggcaa
     8941 ggccacttca ccacactgac gcacggcaat caccgcaggc aagcgaaccc ggcaacagcg
     9001 cagcccatgt tcagtacgct gttcgagggt gataaacggc gcgtcgagag tgaaacgctc
     9061 cacctgggtg aagcaaggcc agcccagcat ttccgccagc aaaaatggcg tttgcccatt
     9121 ctgcccttcg ctgctttggc agccggtaat tatcagatcc agcggattct gacgctgcca
     9181 ttcggcaata tggcgagcga caaattccgg cgcaaagcgc agatctgccg ccgtctccag
     9241 caatacagct tcctcaaacc caagagccat gagatagcgc agccagtgca acgcccgttc
     9301 atcccccatg ctcaacgcgg ttaaagacat cggcgtaccg ttttttctct gcgccaacag
     9361 cagcgcggcg gcagcctgtt catcagcacc gagtaaactt cgcagtagcg aaacatccgg
     9421 tccgctatta ccctgagccg ccgcctgcca ctctttttcc gccagcattc cggcatccgg
     9481 ttcggcttta aacgctaaca gaatgttcat tcgcacctcc tacatttccc gccgcaacca
     9541 gcgggagtgc tttggtttct ggtgcccata gccaggtcac aacaaaacca actaacaaca
     9601 ctgccgccag gatcaataag gtaacctgca ttccccactg cgccagcacc cacggcagca
     9661 ggccagtact taccgccgcc cccaaccgac tcatggcggt ggcaaaaccg acgcccagcg
     9721 agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc caccagatta ctgactgccg
     9781 aaatggtggt gctgaagaga acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac
     9841 aggccattac taccagcgtt gccgccagca gcaaaaaact tcccagcaaa aatttgcgat
     9901 gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc cacaattaac aacgcattaa
     9961 gcatcaggct ggcagtcaac gcatcttcca gaccaatagt ctgggcgata gttggcagcc
    10021 aggtataaat cacaaaccat gggattacga ggcagacaaa gaagacgctg ttaaacgccg
    10081 tgcgccgcca gtagcgcgaa gagaacaagg ttttgatgtg tttatgggtc gccgttgcca
    10141 cttcatcgcc cagtaaaaca tggggaccaa aatagcgatg cacgatagcg tgagcttctg
    10201 caaaacgccc ctggcgcagt agccagcgtg gcgattctgg cgttccccag cgtaataacg
    10261 taatcaacaa cgcgggcata gctgccgatg ccagcagcca gcgccaggcc tccgggcttt
    10321 cgctaataaa gtgatgtccg gcaatacttg ctaacacata gcctacggtc cacaccacgc
    10381 tgaatgcgcc cagcaaaaca ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt
    10441 gacctactga gtaatcgcct cccagaccaa tgccgatcaa aatgcgcagt ccaataagat
    10501 gctctggcgt ggtggcaaaa aattgcagga acgaagcgag cgtaatcagc aaaaagctga
    10561 aggtgaagat tttttgccga ccaatatggt cggagatcca cccaagaacc aggctgccaa
    10621 gaaacaaacc aaggagcgcc gagccgccga tcattcccgc cataaacggc gtcagttgca
    10681 tggcgggcgt aagttgaata atggcgtaac caatgacgcc gagaacataa ccgtcggtca
    10741 ggtgtgcgcc gaaagtgagc gcagcaattc gacagtgaaa acggttaagc ggtaaatcat
    10801 ccattcgcac cggtgaagtg ttcatcttta ccccttcatt gtccggtaat aagaacggct
    10861 tctcgcctga gaagccgaaa aggtaacgcc ccatcatcgc ggaacgctgc ctgacacatt
    10921 atttttcaat cggatagata gtacccgtat tcataatgcc gttaggatcg aattgctttt
    10981 tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag cttgctccag tgaacgcgat
    11041 gtttaccaat gccgtggtgg tgcaccatcg aaccgccgag gcgaatggtt tcttcgcaga
    11101 tgattttgtt gagcggattg tggtacttgt cgatttcctc ttccggctta cagttgacaa
    11161 cgttgtagtc gtagacgaag tacatgttgg tgccgttctg atagctatga gaggagtgac
    11221 cgcccagcat agtgatgtcg tcggcgtgcg ggaactcggt acgaatacgg ttaataacgc
    11281 tttcgtagat ttcgtggatg cagctccagc agccggacac ttcagtggta aagcccatgt
    11341 tgccggtttt gaggatttgc acacgttcgg cagccacttt atccggtccc cagttcaggt
    11401 tgttaaacca ggtttcgatc agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa
    11461 cgatttccgc aatcccttcg cccgtcgcct tcgcaatgcg agggttacct tcagccataa
    11521 agatcagcac gcattttccg tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt
    11581 catacaaacg agcgatcgac ggacgatacc cttccacaat gatttcacgc aggatgttga
    11641 agccggtttt catgtcttcc aggatatagc cgtagaagag gttgttttcc ggggtgaatt
    11701 taaatatttt cactgttact tcagtgatat agcacaatgc accttcgttg ccgatgatga
    11761 tgtgacgaat gtccgggcca gccgcgcggc gtggcacgtt tttaatacgt gtgacggtgc
    11821 catcagccaa tacggcttcc agaccaacga ccatatcttc gattgcgccg tagagtgtgg
    11881 agaactgccc gatactgcgg gttgctacca ggccgcccat ctgcgccagc ggctttgact
    11941 gcggagaatg ccccgtggtg taaccttttt cacgcaacgc gttttccagc acctccagcg
    12001 gaacaccaca ttgcgccgtc gcctgcatat tctcaatatc aatattaatg atttgattca
    12061 tggcggaacc gtcgagcacc accgagtttt ctacaacagt ttccagccca ccttcagtgg
    12121 cggaagcacc ggtacgcggc acaccgttaa ttttgtgcgc attcataaaa ttcagcacac
    12181 gggatacttg ctctgtggaa ccgagtttta cgaccgctgc cggaatcggc aaagtataaa
    12241 tgccatgaat atccggaaat ttacgaaaac ggtcaatact gttcttcttt aatactgttt
    12301 catcggtaat tacgcgatct gcaccaacaa tttcctttag ctggtcgaca atcgctgcgc
    12361 gagataaaga catagtaatt ccttcctgat aataaaaatg gaataatcat taataagaga
    12421 aaacagaaac caccgttatg ggttagcgca ctaaataacc gccatcgacc actaataaat
    12481 gcccgttgac ataattcgat gcctgacttg cgagaaacac ggctgcgccc attaaatcct
    12541 gagtatcgcc ccaacggttt gccggaatat gatcaagaac gcgctgattg gtttctggat
    12601 tactgcgtgt cgccagcgta atatctgttg cataatagcc aggggcgata ccatttacct
    12661 gaatattata ttgacctagt tcatcacaat aagctttggt gaatccggca agagcatgtt
    12721 tagtggcaga atatgcaggt gaccattgtc cacctaagta agagaacaat gaacagatat
    12781 taataatttt accgcttttc tgcgggatca taatttttgc cgcttcgtag cttaactcga
    12841 atgcggcggt caggttcaca tcaatcatcg ggtcccagtc ggctcgacca aagtccagca
    12901 ccttattcag cttacaaata ccggcattgt taaccagaat atcaactgtt ccgaaacgct
    12961 cacagcaagc agcgataatc ttctgcggcg cgccttctgc ggtgatatcc acctgcataa
    13021 aatcaacttc aacaccctgt ttttcaatca tttcctttgt ttcgccgtta tctttgacga
    13081 agctaggaat aaagacattt gcgcctgctt tggccaacgc catggcaaat gcctggccta
    13141 aaccgctatt cccgccggta acaattgcgg ttttaccttt cagggagaaa aaatccattg
    13201 agaacgcatt gagagattcg attgacatag ttagctccaa tttccgtaag gcaaaaaaaa
    13261 agaaaggtaa cttcccccgc gagagggaag ttacctttct cattatctct ggtaactgga
    13321 gataattttt agcataaggg cataactgct aatgtgatcg taatcacagt gtgatattcg
    13381 cattatcaaa tagttttaaa aagcagaagc gtgattactc tcacacaaca cctctgcttt
    13441 ccatgttatt tcttattgca attactagag acctgagaaa agtaatccac ctgaaagggc
    13501 ggcttacttt tctctttttt ttgtctatta caaatgggaa tacgatatgc aacacaactc
    13561 atatcgccgt tggataaccc tcgcgataat tagttttagc ggcggcgtta gtttcgacct
    13621 ggcttattta cgttatattt atcaaattcc catggcgaaa tttatgggat tcagcaatac
    13681 cgagataggt ttaataatga gtacctttgg tattgcggct attattcttt atgcccccag
    13741 cggcgttatt gccgataaat tttcacaccg caaaatgatt acttccgcga tgatcattac
    13801 cggattactg ggtctgttaa tggcaacgta tccaccgctg tgggtaatgc tctgtattca
    13861 ggtcgccttt gcgataacaa cgattttaat gctgtggtcg gtgtcaatta aagccgcatc
    13921 gttgcttggc gatcatagcg agcaagggaa aattatgggc tggatggaag ggctgcgcgg
    13981 cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc ttttctcgct ttgcaccgga
    14041 tgacagcgcc agcctgaaaa cggtcattat catctatagt gtggtttaca tcttgttggg
    14101 gattctgtgc tggttttttg ttagcgataa caacaacctg cgcagtgcca ataacgaaga
    14161 aaaacaatca ttccagctta gcgacatcct ggccgttttg cgcatcagca ccacctggta
    14221 ttgcagcatg gtaatttttg gtgtcttcac catctacgcc attctgagtt actccaccaa
    14281 ctatctgacc gaaatgtatg gcatgtcgct ggtggcggcg agctacatgg ggattgtgat
    14341 caacaaaatc ttccgcgcgc tgtgcggccc acttggcggc attatcacca cctacagtaa
    14401 agtgaaatcc cctacccgcg tgatccaaat cctttccgta ctcggcctgc tggcgttaac
    14461 tgccctgctc gtcacgaact ctaacccgca atcggtcgcg atggggattg gcctgatttt
    14521 actgctggga ttcacctgtt acgcctcacg cgggctgtac tgggcctgcc ctggcgaagc
    14581 gagaacaccg tcttacatta tgggcaccac ggtaggtatt tgttcggtga ttggattcct
    14641 gccggatgtc ttcgtttacc cgattatcgg ccactggcag gacaccctgc ccgcagcaga
    14701 agcctaccgc aacatgtggc tgatgggcat ggcggcgctc ggcatggtga tcgtctttac
    14761 ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc cccgcaatag ctagcagcaa
    14821 gtaagccagg aaaattacag gagaaaaatc atgtcgaaaa aatacatcat agggattgat
    14881 ggcggaagtc agagcacaaa agtggtgatg tacgatctgg aaggtaatgt ggtttgcgaa
    14941 ggcaaaggtt tattgcagcc gatgcacacg ccagatgccg ataccgcaga acatcctgac
    15001 gacgatttat gggcatcatt atgttttgcc ggtcacgatt tgatgagtca gtttgccggg
    15061 aataaagaag atattatcgg tattggtctg ggatccatcc gttgctgccg tgcgttattg
    15121 aaagccgatg gcacgccagc tgcgccattg attagctggc aggatgcacg cgtgacgcgt
    15181 ccctacgaac ctacgaatcc tgacgtggca tatgtcacct ctttttcggg ttatctaacg
    15241 catcgcttaa ccggcgagtt taaagacaat atcgccaact attttggtca gtggccagtg
    15301 gattataaga gctgggcatg gagcgaagat gctgcggtaa tggagaagtt taatatccct
    15361 cgtcatatgc tgtttgatgt gcaaatgcct ggcaccgtcc tcggacatat cacaccacaa
    15421 gccgcactgg cgacacattt cccggcaggg ctgccggttg tttgtaccac cagtgataaa
    15481 ccggtagaag ctctgggagc cggattactg gatgatgaaa cggcggtgat ttctcttggc
    15541 acctacatcg cgctgatgat gaacggtaaa gcactgccga aagatccggt ggcgtactgg
    15601 ccgattatgt cttctatacc ggaaacattg ctgtatgaag ggtacggtat ccgtaaaggc
    15661 atgtggacgg tgagctggct acgcgacatg ttaggcgagt cgttaattca ggatgccaaa
    15721 gcacaggatc tttcaccgga agatttgctc aacaaaaaag cttcttgtgt gccgcctggc
    15781 tgtaatggtc tgatgacggt gctggactgg ctgaccaacc cgtgggaacc ctacaaacgt
    15841 gggattatga tcggctttga ttccagcatg gattacacat ggatatatcg ttcgatactg
    15901 gaaagcgtgg cgctgacgct gaagaacaat tacgacaata tgtgtaatga aatgaatcac
    15961 tttgcaaagc atgtgatcat tactggcggc ggttcgaaca gcgatctatt tatgcaaatt
    16021 tttgccgacg tgttcaacct tccggcacgc cgtaacgcca ttaacggttg tgcaagtctg
    16081 ggggcagcga tcaatacggc ggtaggtctg gggctatacc cggattacgc aacggctgtc
    16141 gacaaaatgg ttcgcgtgaa agatatcttt atgcctgttg agagcaatgc caaacgctac
    16201 gacgcgatga ataaaggtat tttcaaagcc ctaaccaaac atactgatgt gatcctgaaa
    16261 aaatcgtatg aagtgatgca tggggaattg gggaatgcgg attcgatcca gagctggtcg
    16321 aatgcgtaag tgaggaagac cgggcgggaa actgcccggc ctgaacatac ctgaatggtt
    16381 atccccgctg gcgcggggaa cataagttcg acaacttaaa aaatgataac aagtccggtt
    16441 tatccccgct gatgcaggga acataataaa aactttaact agttcggttt atccctgttt
    16501 ttacaaggaa tattaactac actcgatgtc tttagaataa aagacaatac gaatcgcact
    16561 ttatcaattt tcacttaaag tatgaactgg atatctttat tcataagaac tattcatcaa
    16621 cagcgtatag aggcggttat gtaaaaccac tcattagcct caaaacaaag acaaatttcc
    16681 agatttatta cttaatcaat gaat
//
LOCUS       DAZFFG010000099        16480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFFG010000099 DAZFFG010000000
VERSION     DAZFFG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.099"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(178..792)
                     /gene="yagU"
                     /locus_tag="ACHJ7J_003686"
     CDS             complement(178..792)
                     /gene="yagU"
                     /locus_tag="ACHJ7J_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO5001868.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            1210..1899
                     /gene="paoA"
                     /locus_tag="ACHJ7J_003687"
     CDS             1210..1899
                     /gene="paoA"
                     /locus_tag="ACHJ7J_003687"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO5001869.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLSGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            1896..2852
                     /gene="paoB"
                     /locus_tag="ACHJ7J_003688"
     CDS             1896..2852
                     /gene="paoB"
                     /locus_tag="ACHJ7J_003688"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO5001870.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAVDAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            2849..5047
                     /gene="paoC"
                     /locus_tag="ACHJ7J_003689"
     CDS             2849..5047
                     /gene="paoC"
                     /locus_tag="ACHJ7J_003689"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO5001871.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPYDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASTVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            5057..6013
                     /gene="paoD"
                     /locus_tag="ACHJ7J_003690"
     CDS             5057..6013
                     /gene="paoD"
                     /locus_tag="ACHJ7J_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO5001872.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCEREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSYLPPSS"
     gene            complement(6192..7319)
                     /locus_tag="ACHJ7J_003691"
     CDS             complement(6192..7319)
                     /locus_tag="ACHJ7J_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5001873.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(7461..8519)
                     /locus_tag="ACHJ7J_003692"
     CDS             complement(7461..8519)
                     /locus_tag="ACHJ7J_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5001874.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDVAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHLVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            8784..9668
                     /locus_tag="ACHJ7J_003693"
     CDS             8784..9668
                     /locus_tag="ACHJ7J_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5001875.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHQ
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            9911..9967
                     /locus_tag="ACHJ7J_003694"
     CDS             9911..9967
                     /locus_tag="ACHJ7J_003694"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001876.1"
                     /translation="MVVFVATVEYAYLSLHFY"
     gene            complement(10371..10649)
                     /locus_tag="ACHJ7J_003695"
     CDS             complement(10371..10649)
                     /locus_tag="ACHJ7J_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001877.1"
                     /translation="MPMYIKLILSIVRRISVNTICSLIVVVTLSLLSFSSVAKTITAV
                     GSTIDSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(10814..11080)
                     /locus_tag="ACHJ7J_003696"
                     /pseudo
     CDS             complement(10814..11080)
                     /locus_tag="ACHJ7J_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            11086..11493
                     /locus_tag="ACHJ7J_003697"
     CDS             11086..11493
                     /locus_tag="ACHJ7J_003697"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5001878.1"
                     /translation="MVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEE
                     NRRAFDLAIEPQITTNDLRLMLRLALAGGGITIATQETFRPYIESGRLVSLLDDFLPQ
                     FPGFYLYFPQRRNIAPKLRALIDHVKEWRQQLA"
     gene            11831..13150
                     /locus_tag="ACHJ7J_003698"
     CDS             11831..13150
                     /locus_tag="ACHJ7J_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5001879.1"
                     /translation="MTFTITQDRQLASLTVTKEEKEILFAVKSKAGGGLFIRVRYGSD
                     NKSWIYRYRIAQKPAKLTLGSYPAMGLAQARKAHADAIELVRKGIDPRHIRKNEKQKN
                     EQMIIYSELWKDWIEFRKTSKPISTRTLADYEGTYHRHLEKTLGNLRIDELTKSVIFE
                     HLSNVRKTSSEAVRKGLIILNMTLDHANLQGMLEHNPARLLKPAMFGASMNKPRERWL
                     PENELRMLWKALDDATVGGGSIASGGYGISSSVVLSHSVANALRLIIFTGVRRSEAAQ
                     MRWDQINGERWTIPATKNGKSHIVTLHPYMLSLIKTQREFTEGAYVFGSTSKPGFPIT
                     NDALTRALERVRSKYLAELTPFSPHDLRRSVATGCAEYLDAPERLIELLLNHVPKDRL
                     IRTYQVGQQAEKLKNLFIEWGNFIMNNVITVQQNAPDNVIHIKFGNR"
     gene            complement(13243..14091)
                     /locus_tag="ACHJ7J_003699"
     CDS             complement(13243..14091)
                     /locus_tag="ACHJ7J_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5001880.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDVQARDTWYSSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVSWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKYYPE
                     IIPYNI"
     gene            complement(14176..14373)
                     /locus_tag="ACHJ7J_003700"
     CDS             complement(14176..14373)
                     /locus_tag="ACHJ7J_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5001881.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESEIIFDNDEDKTDSAALLPC
                     IEQAKEDIRTLRQLQFLHQNR"
     gene            complement(14385..14873)
                     /locus_tag="ACHJ7J_003701"
     CDS             complement(14385..14873)
                     /locus_tag="ACHJ7J_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5001882.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVACDNGYS
                     LRVVDESDRTSAERTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTGAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAGCLPGLPTF
                     DW"
     gene            complement(14870..15247)
                     /gene="cbtA"
                     /locus_tag="ACHJ7J_003702"
     CDS             complement(14870..15247)
                     /gene="cbtA"
                     /locus_tag="ACHJ7J_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO5001883.1"
                     /translation="MKTLPVLPGQTTSSRPSPVEIWQILLARLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCNHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKQ"
     gene            complement(15337..15705)
                     /locus_tag="ACHJ7J_003703"
     CDS             complement(15337..15705)
                     /locus_tag="ACHJ7J_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5001884.1"
                     /translation="MSDTLPETNNPNDNHDRPWWGLPCTATPCFGARLVQEGNQLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYLAVYTTPETKK"
     gene            complement(15755..16402)
                     /locus_tag="ACHJ7J_003704"
     CDS             complement(15755..16402)
                     /locus_tag="ACHJ7J_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFO5001885.1"
                     /translation="MSRIIATTVYTLDELSCPAAREKARDWYRVHHSDSNWYENVYED
                     FREVCDIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQSATARRI
                     REHAPQERELHRIADTLQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAM
                     TADAEAVVSEALRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLRVG"
BASE COUNT         4140 a         4257 c         4194 g         3889 t
ORIGIN      
        1 cccgatactt cttctatttg ccagcaacaa tgcctctctt ctgtagcgtt cttcggatct
       61 acatcatcct gaagtagcgc gatatactca ctgactatct gaatcagaat ataaaaagca
      121 atgtttttaa cctataaaaa tggcgctgta tttgcgccat ttttatcatt caatgcatta
      181 tctgtttgag cctaaaggga tctcagggtc tggctcatga gtaattctgt ttcgtaaatc
      241 tctgcgaata atttcaatgg accagaacca gactaaatgt ccaaaaattt cagaaacatt
      301 ctcataccac gggagatcaa acagaggtgg tgtcagtccc atgagaggga atgaaatcat
      361 atgaacaaaa agttgggcta aagcacctgc cagtaaaccc tgccaaagtt taatttttgg
      421 aaatacttcg gcgaccacac aataaccgac agcaaacact atcgaaaaga taatgtgcgt
      481 aacaccaacc cagttaaaga catgcccggc aaaggtataa acagccgcat tgggatctgt
      541 cagccccagc cagtctcgaa gaaaaatata cggaggattg agaaaattac gcgagcaatc
      601 aatttgccca gcagccctga ttaatgattc cgggccacac gctgcattaa acatatccac
      661 cgggctacgt ggcggcaatg gaacttcagc cccccacttc acaaatgcgg aaacaacgcc
      721 agcaatcagc ccaatgaatg cagcaagacc ataacgtctg cggttcggtg gagtttgttc
      781 aaatatattc atatctaccc tgcttgtacc attatgttat acacctcttt aggagtattc
      841 ataaaacaag gtaaatgtaa agaactgtat tgttttgtat aacaagatgg ttgtctaatc
      901 accaaagaat ataagcacca tcatttctcc ctatttttat attagaagtg acatagattt
      961 gcagggtgat gcagagctga caatcacagg tttccttatc ggtttttgca tcgtacaact
     1021 aaagcaataa accagctccc atcatcatca acatcccggc gaacatgaat ttattagccg
     1081 ggctcatagc tgcattcgcg ttttaagaat aatcctcctg ctgtcgccga ctatgcttaa
     1141 cgtttaaaaa agcatcagca ctctcgcaac gcactcttat tttccccttt agaataccgg
     1201 aggcctggta tgagcaacca aggcgaatac cccgaagata atcgggttgg gaagcacgag
     1261 ccgcacgatt taagtttgac ccgtcgcgat ctgattaaag tgagcgccgc aacggcggcg
     1321 gccgccgtgg tttatcctca ttctacgctt gcggcaagcg ttccggcagc tacacccgcg
     1381 cctgagataa tgcccctgac acttaaggtg aacggcaaaa ccgagcagct tgaagtggat
     1441 acccgaacca cgctactgga cgctttgcgt gaaaatctgc atttgagcgg taccaagaaa
     1501 ggttgcgatc acggacagtg cggagcctgt accgtgctgg tcaatggtcg caggcttaat
     1561 gcctgcctga cgcttgcagt catgcatcag ggggccgaga tcaccaccat tgaaggcctg
     1621 ggttcaccag ataatcttca ccccatgcag gcggccttta tcaagcatga tggcttccag
     1681 tgcggctact gcacctccgg gcaaatttgc tcatcagtag cggtgctaaa agagattcag
     1741 gacggcattc ccagtcacgt cacggtcgat ttggtttccg ctccagaaac aactgccgat
     1801 gagatccgtg aacgtatgag cggcaacatc tgtcgctgtg gtgcatacgc taacatcctt
     1861 gccgccattg aagatgctgc gggggagata aaatcatgaa ggcgtttacc tatgaacgag
     1921 tgaacacccc ggccgaggcg gcacttagcg ctcagcgcgt acccggcgca aaatttattg
     1981 cgggcgggac caatctgctg gacctgatga agctggaaat tgaaacgccc acccacctta
     2041 tcgatgtgaa cgggctcggg ctcgataaga ttgaagtgac cgacgcgggt gggctgcgca
     2101 tcggcgcact ggtacggaac accgacctgg cggctcacga gcgcgtgcgt cgtgattacg
     2161 cggtactctc ccgcgccctg ctcgctggcg cgtctggtca gttacgtaat caggcaacca
     2221 ccgcaggtaa tctgctccag cgcacgcgct gcccctattt ttacgacacc aatcagccct
     2281 gcaataagcg cctgcccggg agcggctgcg cggcgcttga aggctttagc cgtcagcacg
     2341 cggtggtagg cgtaagcgaa gcctgcattg ccacccatcc gagcgatatg gcggtcgcaa
     2401 tgcggttgct ggatgcggtg gtggaaacca tcacgccgga gggaaagact cgcagtatca
     2461 cactggctga tttttatcac cctccgggaa aaacgccgca cattgaaacc gccctgcttc
     2521 ccggtgagct tatcgttgcg gtgacgttac ctccgccgct cggcggaaaa catatttacc
     2581 gtaaggtgcg cgatcgcgcc tcctacgcct ttgccctggt atcggtcgcg gcgattattc
     2641 agcctgacgg cagcgggcgc gtcgcgctgg gcggagtagc acataagccc tggcgcattg
     2701 aggctgtgga tgctcaacta tcccaggggg cacaggccgt atatgacgcg ctgttcgcca
     2761 gcgcccatcc caccgctgaa aacaccttta aactcctgtt ggcgaagcga acgcttgcct
     2821 ccgtactggc tgaagcgagg gcacaggcat gaaatttgat aaacccgcag gggaaaaccc
     2881 gatcgatcag ctgaaggttg tcggtcgtcc ctatgaccgc atcgacggac cgctgaaaac
     2941 taccggcacg gcacgctacg cctacgaatg gcatgaagaa tcccccaacg ccgcctatgg
     3001 ctatatcgtc ggttccgcca ttgccaaagg acgcctcacc gcccttgata cggacgccgc
     3061 gcaaaaagcg ccgggcgtac tggctgtcat taccgccagt aacgccgggg cactcagtaa
     3121 aggcgacaaa aacaccgcca ggctgttagg cggccccact attgagcact atcatcaggc
     3181 cattgcgctg gtagtggccg agaccttcga acaggcgcga gcagcggcct cgctggtgca
     3241 ggcgcactat cgccgtaata aaggagctta ctctctggcg gacgaaaaac aggccgtcag
     3301 tcagccgccg gaagacacgc ccgacaaaaa cgtcggtgac tttgacgggg cattcacctc
     3361 cgctgcggtg aagattgatg ctacctacac gaccccggac cagagccata tggcgatgga
     3421 gccgcatgcc tcgatggcag tctgggatgg aaataagctt actctctgga cctcaaatca
     3481 gatgattgac tggtgccgca ccgatctggc aaaaacgctg aaagtgcccg tggagaatgt
     3541 gcgtattatc tccccgtata tcggcggagg gtttggcggc aagctgttcc tgagaagcga
     3601 tgcgctgctg gcggccctcg ccgcccgagc ggtgaaacgt ccggttaaag tgatgctccc
     3661 ccgcccctct attcccaata acaccacgca ccgccccgca acccttcagc acttgcgtat
     3721 cggcgctgac cagagcggga aaatcaccgc tatctcacat gaaagctggt ccggaaacct
     3781 gcccggcggc acgccggaaa cggcggtaca gcaaagcgaa ttactctacg caggggcaaa
     3841 ccgtcatacc ggcctgcggc tcgccacgct tgatttgccg gaagggaacg ccatgcgtgc
     3901 gcccggcgaa gcccccggtc tgatggcgct cgaaatcgcg atcgacgaac tggcggaaaa
     3961 agcgggcatc gatcccgtcg agtttcgcat cctgaatgac actcaggttg accccgccga
     4021 cccgacgcgc cgcttctctc gccgtcagct tatcgagtgc ttgcgcaccg gagcggataa
     4081 atttggctgg aagcagcgca acgccacacc cggacaggtg cgcgacgggg agtggctggt
     4141 cggccacggt gttgcggcgg gctttcgcaa taatctgctg gaaaaatcgg gtgctcgggt
     4201 tcacctcgaa caaaacggca ccgttaccgt agaaacggac atgaccgaca ttggcaccgg
     4261 cagctacacc attctggccc agacggcagc ggaaatgctt ggcgtaccgc tggagcaggt
     4321 tgcggttcac ctcggcgatt ccagtttccc ggtttctgcg ggttctggtg gacaatgggg
     4381 cgcgaatacc tccacctccg gcgtttacgc cgcctgtatg aagcttcgcg aaatgattgc
     4441 ctcgacagtc gggtttgatc ctgagcagtc gcagtttgcc gacggcaaga ttaccaacgg
     4501 tacccgaagc gccacgctac atgaggccac cgcaggcggc agactgacag cggaagagag
     4561 cattgaattc ggaacactga gcaaggagta ccagcagtcg acctttgccg ggcattttgt
     4621 ggaggtcggc gtgcatagcg cgacgggaga agttcgggtc cggcgtatgc tcgctgtgtg
     4681 tgctgcagga cgcatcctga atccgaaaac tgcacgcagc caggtcattg gcgcaatgac
     4741 tatgggcatg ggcgcggcac tgatggagga gctggcggtg gatgaccgtt tgggctactt
     4801 cgttaatcac gatatggcgg ggtatgaggt gccggtccat gcggatatcc caaaacagga
     4861 ggtgattttc ctggatgata ccgaccccat atcctccccg atgaaggcca aaggtgtcgg
     4921 tgagctgggc ctgtgcggcg tgagcgcggc tatcgccaac gcggtgtata acgccaccgg
     4981 tattcgggta cgggattatc ccatcactct ggataagctg ctcgataagc tgccggatgt
     5041 ggtttaagga ggaacaatgt catacccgct ttttgacaaa gacgaacact ggcataagcc
     5101 agagcaggcg tttctcaccg atgaccaccg gaccattctg cgcttcgccg tagaggcgct
     5161 aatgtccggt aaaggagcgg tgctggtgac gctggtggag atacgcggcg gcgcggcgcg
     5221 cccgctcggg gcgcagatgg tggtgcgcga agatggtcgt tactgcggtt ttgtctctgg
     5281 cggctgcgtg gaggccgctg ccgcttttga agcgctggag atgatgggct caggctgcga
     5341 acgcgaaatt cgctatggcg aaggttcgcc gtggtttgac atcgttctgc cctgcggcgg
     5401 tgggatcacg ctgacgctcc ataaactacg ctcggcacag cctctgctcg ccgtgctgaa
     5461 ccgcctggaa cagagaaaac cggcggggct gcgctacgat ccgcaagcac aatcgttggt
     5521 gtgcctgccc acgcaaaccc gaacgggctg gaatctcaat ggctttgagg tggggttcag
     5581 gccatgcgtc aggctgatga tttacggacg ttctcttgag gcgcaggcaa ccgcgagtct
     5641 tgcagcagcc acaggctatg acagccatat cttcgatctt tttccggcct cagccagcgc
     5701 tcagatcgat accgatacgg cggtcatttt gctgtgccat gatctcaacc gagagctgcc
     5761 agtgttgcag gccgcgcgag aggcaaaacc cttttatctc ggcgcattgg gcagctatcg
     5821 aacccacacg ttacgtctgc aaaagctcca cgagctggga tggtccaggg aggagacagc
     5881 gcaaatccgg gcacccgtcg ggatatttcc caaagcccgg gatgcgcata ctctggcact
     5941 ctccgtgctg gcagaagtcg cctctgtacg tctccatcag gaggaggatt catacctgcc
     6001 cccgtcgtcc tgatccttgc ggccgggcgt gggaagcgct ttctcacctc cgggggaaat
     6061 accataaatt aagagaaaaa tctgcagtag ccgttttaga aaatcaccta aatcgtttaa
     6121 aagagcgccc tacccatagt gggtgctcat cgccttgtgt aacttattca gcaacgcgcg
     6181 ccctcgccgg actaaagcgc accggccttc tggcgtgcgg taacaaaggt cagtacgccc
     6241 gcgcaaagga gcagcacact actcatggca aacgtactct gccagccaag gcggtcaaac
     6301 acgatgcctc ctgccgttga tccaagggcg attgagagct gaatcaccgc gaccatgagc
     6361 ccgcctccgg cttcggcgtt gtcaggcaac gtacgcgcaa tccatgtcca ccatccggtt
     6421 ggcgcggcgg tggccagcat gccccagagt cctaacagca gcgagaccgt ccagacgtga
     6481 tgcccggtaa gcatcagacc gatggcaatg acggccatca gtccgggtat ggccatcagc
     6541 gtggggtaga attttctgtt gagaaatgtc gaaacaacca gcgtgccaat aaaaccggcc
     6601 acgccgataa tcagcaaaat caacgacaag cctaaggaat taacccgtgt caccgactcc
     6661 agaaacgggc gcacataggt gaatagcgca aactgcccca tgaaaaacag gccacaagct
     6721 atcatgccga ccacaaccat gcggcggcta aataagcgga ataccgcgcc acatgacgtg
     6781 tggcttttat tcgcatccat gctgggcaga ctaatgcact gccagataaa ggcgacaacc
     6841 gccatgggca ccaggcacaa gaaagcgcct cgccatccga ccgtagcccc cagatagctt
     6901 cccagcggcg cggccacgac cgtcgccagc gcattaccgg cgttgaaaat cgccagcgca
     6961 cgcgtgacct gatgctgcgg caccagacga atcgccgttg ccgctgacat cgaccagaat
     7021 ccgccaatgg ccacaccaat catcgcacga ccgaccatat acatcagata actggtagcc
     7081 agcgcaataa taagccccga tacggccatc agaaccgtca tccccagcag caggaactta
     7141 cgattcatct ttccagctag cgttgaaagc gtcaggctgg tcagaaccgc cagcgcgccg
     7201 gagatagcaa tcccctgccc tgccagcccc tccgtcacgc ctaaatcgcg ggtaatcggc
     7261 gtgagcaggc tgacgggcat aaattcagaa gcaatcagca caaacacgca cagggtcatg
     7321 gcaaaaatac cgccccagta tgcgcgctga tgatgaggtg atagtgtttg gcttagtgtc
     7381 gacatagtga aatttcacaa agatcaggat aaaagccacc agcctatggt cggtggcttt
     7441 gcatcaattc aggagaggtt ttatttcagt ttggtctgga aaaattgttc gaacttcgcg
     7501 aaaggtattt ttccggccac gttatcgtac aggtccacgt gatttgcccc tggtaccacc
     7561 accaactctt tgtctttact gccaaccgcc ttgtaagcat cttcagcaaa atagcgtgaa
     7621 tgtgctttct caccggtcac aatcagcgtc ggaatggtta tttcactggc gtagctcaac
     7681 agcggcatat tcataaatga cagcggcatc gttgccgtcc acgccccggt agagttaacg
     7741 gaacgttcgt ggaagccgcg cggcatgcga tagtagtcaa agaactcttt cagtaccaga
     7801 tgaggatttg ccggcagcgt ttctggcaga atgcgctcgc ccgcactgac cttgccgttg
     7861 ctgtcgacat taatatcatg accaccgtga gcgaacgttc cgctctgcgc atccttccag
     7921 cgctgttcat tcagatattg cagaacagca cgacggtcgg cggtagaata acggtcttcg
     7981 ccatccccca caccatggcc catcgcccgg ctcatgtcat acatcacgct ggtagcaacc
     8041 gctttaacgc gggtatccat cgccacgtcg ttcagcgcca tgccacccca gccacaaata
     8101 ccgagcagcc cgatacggtt gcgatctacc tctttttgca gccctaagaa atccaccgcc
     8161 gcgctgaaat cttcagtgtt gatatccggt gaggcgacgt tccgcggata gccgccgctt
     8221 tccccggtgt aagaaggatc gaacgccagg gtaacaaacc ctcgttccgc cagcgtctgc
     8281 gcatacaagc cgctggattg ctctttcacc gcgccaaatg gcccactgac cgcaatcgcc
     8341 gccagcttgc gatcgccacg gtctttaggc aggtaaagat cgcccactaa ggtgatgcca
     8401 tatcggttcg ggaacgatac tttacggtga tcgactttct ggctctcggc gaacgtttta
     8461 tcccatttat cggtcatgga cacaggggca ttaggattgg ttgaatcagc ataactcatt
     8521 gtcgtgactc cacttaatga tgcgaaaagc agcatggccg gcatcgcggt ttttagcgtt
     8581 cgggtgaagg ctttcataaa aatccccata aaaaacaaca gagagataca ggtttccgac
     8641 ggacaacgct gcggtgcttt actttgatgg ggtcattata gtgagcagaa ttagtgctga
     8701 ttagggggcg attaatgcta agattaatac catattgtta taaattaatg cgaagattgc
     8761 cggcatgaag gggggatgtt tcaatggcga aaagggagaa ctacaacgag ctgtacctgt
     8821 ttatgcaggt ggtgcgagaa ggcagcttta ccgcagcggc tcagcggctg ggtcttgctc
     8881 agtcaggagt cagccgttct gttcgcgagc ttgaagaaag gctgggtgtc cagctgctag
     8941 tgcgcaccac gcgtaaactg tcgctgacac aagcaggcga gcagctctac cagaagacgg
     9001 catctggatt tgaaatgtta gatcttgggc ttgccacact ggcgcactat cgagagacgc
     9061 cctccggcac ggtgcgtatc aatgccagcc agcacgcgat tgataaatgc ctgctgccaa
     9121 agctggcggt atttaaacag cgatatcctg atatccggct tgaactgata aatgagagcc
     9181 ggttcgtcga tatcatcgag gaaagattcg atgcaggtgt ccgcctgggg cctgaagtcg
     9241 gccagggtat ggtcgcggta cgcatcacgc ccgatatgga gatggcaatt gtggggactc
     9301 cggaacattt tcgtcgctat ggttttccgc aaactccggc ggatttaaag gcgcatccct
     9361 gtatcgccta tcagtttgcc gacggcagcg tataccagtg ggaactcaat caggatgata
     9421 aaaaaatcac tcatcaaccc gaaggacaat gggccctatc cgacagctat atggaagcag
     9481 aagccgcccg gctgggcctc ggattggcct atgttcctgt tgaactggtc gcggatgatt
     9541 tagaacacgg taagcttatc agggtgttgc agcgttacag cctgcgaatg gagggattgt
     9601 ttctctatta ccctcatcgc aacgtctccc ccgctctgcg gatggtcatt gatacattga
     9661 aaatctgaac gttaacaacg accgactctt cacggtgcaa tattgtgtca ggtagtcact
     9721 ctgcgttcaa ttccagggtc tgctttgagc gagaaagagc agacttatct gtacaagggg
     9781 actatccagc aatggagatc atccaaaaga aaaaaccacc tggctcccct cgacaacctt
     9841 aatccgggca tttagcctca atcaacattt tcatatctgt caggttactt cattatgaaa
     9901 accgatcaat atcgttgttt ttgttgcaac agtagaatac gcgtatttat ccctccattt
     9961 ctattaataa cggcaataaa ctgttcattt cactgatatt taaaatatgc atccttttca
    10021 agctgttcag cggcgtaaga aaacgtatta aatatgaatt cgacgattta accatttctg
    10081 tctgattggt aatcatctgt gattaatgct ggtctattga ttacgccagc attaaccgct
    10141 tagggatgta ttcttcctga tcatatacag ccctcgaaaa agagagaaaa taaaactgtg
    10201 tccaatatta ctctaccact ccagaccgaa gcatcggtag aattagcaga acgactggta
    10261 caatagattg ggatagcgtc atttgtcagg tattccattg cctgacaaat gacaattttg
    10321 agtcaataga cgtgcttctt gatcatgtct taaaaattat accatgagcc ttatgaagca
    10381 tcatttttac tgactggtgt taattttgct atcatataaa ctctgttctt aaaaaacgcg
    10441 cccagaattt tatatgattt cccttgtttt tctgcctgta aagaaatttc tttttcagtg
    10501 ctgtcaatgg ttgaaccgac ggcagtaatc gttttggcga cgctactgaa tgataataaa
    10561 gacagtgtaa caacaacaat taaagaacag atagtattca cgctaattct cctgacaatt
    10621 gagaggatga gttttatata cataggtatt aaacatgagt catatttacc ccaagagaaa
    10681 atactccttc tgtaattaaa ccttcatctc cttcggggtg agcaatacat aatacatctt
    10741 gctatgaaaa cacagggata tataaatctt ccttctgacc tatttaataa aacgtgaatg
    10801 taaaagttat tcttcagttt gcagatgcag tgggtctgat ggtaatttcg gttgtatcaa
    10861 cgctttcggg agattcaata atatgcctta ccgcacgggc aatatccgca ggttgaagtg
    10921 caacagaacg gtaggcgtcc attgccttca tggtttccac atgggtaatg gttgaagcca
    10981 gttcactttc gacaacgcca ggatttacgc aggtcacacg gattttcgag ctttcctgcc
    11041 gtaagccatc agaaatagcc cgtactgcaa atcaggcagc gtgaaatggt cgttgcctct
    11101 ccctcctatc tcgcagccaa cagcacacct gttcatccgc gtgaacttgt gaatcataag
    11161 tgtatcggct ggcgtcaatc cccggatgtt gcgccttatc gctggccatt tgaagaaaac
    11221 aggagagctt tcgaccttgc gattgaaccg cagattacga ctaatgatct gcgtctgatg
    11281 ttgaggctgg ctcttgccgg cggaggaata acaattgcca ctcaggaaac tttcaggcca
    11341 tatattgaaa gcggtaggct tgtatctctg cttgatgact ttcttccaca atttccgggc
    11401 ttctatctgt atttcccaca gcgtcgcaat attgcaccaa agctccgcgc cctgattgac
    11461 cacgtcaagg aatggcggca gcaattggct taaatgtctg cacctgcatt gcctgatgtc
    11521 agaacagtat tttgattaat tgccagggtt acaatggcat aaatacggca caggaggaaa
    11581 cgtggtgtat ttgaatatgg ggtaactact tgatttaaat ggtgccgata ataggagtcg
    11641 aacctacgac cttcgcatta cgaattagcg agaccagtga ttcaagatgt tcacagccgt
    11701 ttccacatac aataaaaaat atataaaaat caataaaata tacataaatg cagtatcaag
    11761 atgtttcgcc ttgtttctga tgatatcatg ttaaatgtgt accccactgt gtacccaaaa
    11821 ggctgaagat atgactttta ctattacaca agacagacaa ttggcttcat tgaccgttac
    11881 taaagaagaa aaagaaatct tatttgctgt aaaatctaaa gctgggggag gactctttat
    11941 acgcgttcgt tatggtagcg acaataaaag ctggatttat cgttatcgaa tagctcaaaa
    12001 gccagctaaa ttaacactag gtagttaccc tgccatggga ttagctcaag ctcgtaaagc
    12061 tcatgcggat gcaattgaat tagtaagaaa aggtatagat cctcgtcata ttagaaaaaa
    12121 tgaaaaacaa aaaaatgaac aaatgattat ttactctgaa ttatggaaag attggataga
    12181 attcagaaag acaagtaaac caattagcac cagaaccctt gcagattatg aagggactta
    12241 tcaccgccac cttgaaaaaa cattaggaaa ccttcgcatt gacgaactta ccaaaagcgt
    12301 tatttttgaa catctcagta atgtaagaaa aaccagttct gaagcagttc gtaaaggatt
    12361 aattattcta aatatgactc ttgatcatgc caacttacag ggtatgcttg agcataaccc
    12421 agcacgactg ctcaaaccag caatgtttgg tgcatcgatg aataaacctc gcgagcgatg
    12481 gcttcctgag aatgaactcc ggatgctatg gaaggctttg gatgatgcta ctgttggagg
    12541 aggctccatt gcatctggtg gctatggaat ttcctctagt gtggtattat ctcattctgt
    12601 cgcaaatgca ctccgtttga ttattttcac aggagtgcgc cgctctgaag cggcacaaat
    12661 gcgctgggat caaattaatg gagagcgttg gaccatacca gccactaaaa atgggaaaag
    12721 ccatattgtt actttacatc cgtatatgct ttctttaata aaaacacaac gtgaattcac
    12781 tgaaggtgca tatgtttttg gttctactag caaacctggc tttcccatca caaatgacgc
    12841 tctgactcgc gcgttagaaa gggttcgctc taagtatcta gccgaactta ctccattttc
    12901 tcctcatgat ctgcgtagaa gtgttgcgac tggatgcgct gaataccttg atgctccaga
    12961 gcgcctaata gagttactgt taaatcatgt acccaaagac cgtttaatac gaacatatca
    13021 agtcggacaa caagctgaaa aattaaaaaa tttatttata gaatggggga attttattat
    13081 gaataacgtc attactgtgc agcagaatgc tccggataat gttattcata taaaatttgg
    13141 gaacagatga taaaaataca tgcatggtct gagataccct gtctactcga ccatgcagta
    13201 tggttcaact aaatcagtca caacaagaaa tgattagttt tattagatat tgtatgggat
    13261 tatttctgga taatacttcg caatgatgtc atttagccga tcaaccagtt ctggttttct
    13321 gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct catcttcata
    13381 gcaggctttg ccctgaacgg agcggatgtg atcatccagc cgaataataa tattttcccg
    13441 gctgtcgggg atcggtttgc cgctgaacag gtgcagcatt ctttccaggt cggcaagtcg
    13501 atctgcctgt tgcccggtga taagatgcaa tccccaccgg tcccaactca ccagattgtt
    13561 gacgataatt ttactgccaa atttacaggg gcaattggtt ttgtaattcc agttcagccc
    13621 tctgaagaca ttgatcaccc ctcgctcaaa cacttcatct ttgttctggt gcagttgttc
    13681 aaatgtgctc agaatgttcg cttcactgat ttccggaaaa ttatcgtact ccagtgagct
    13741 gtaccatgta tcacgggcct gcacatccat taatgacagc atcccggaac gttgcatcag
    13801 gtcacgccag atttcgcggt caagattgcg ggtgatggct ttcattgcgg tttccggttt
    13861 ctccatgagc cagcagccac agcgaaaatc ctgtttcatg gcccagtcca gtgcggtttt
    13921 accgccaata ctttctgtca ttgctgagat ttcagccagt tgccggataa gtgtgtcgat
    13981 ttgcagcagt gtactctttc gtacagccag aatgtggcgg atactggttg agcaaataac
    14041 gtccgtatgc ccggtgacca cttccggacg ggagattgtg atgtctgaca taatcggttt
    14101 cccttttggt ttataaacga aaacgccagc cctgttcagg ctgacgtcag tggaatgaag
    14161 tctggtgagt gagattcacc ggttctggtg cagaaactgc agttggcgca gggtacggat
    14221 atcttccttg gcctgttcaa tgcagggcag cagtgctgcc gaatccgttt tatcctcatc
    14281 gttgtcaaag ataatttccg attcgcagtc aatattgtcc tgcagccacg caatgaggat
    14341 atccagcgct gtttccgtgg ttaatgattt catgttgttc ctggttacca gtcgaacgtg
    14401 ggtaaacccg gcagacatcc ggcactggca tccagatgaa tgagactgac gccataacgt
    14461 cgggtaagtg tgacaaccag acggcggaac gtttttgaca gccccaggcg tttaagccgc
    14521 agaaccgggt atgaccacgc accggtacgt aacagatatc cgctacctgt gaaatgaatc
    14581 cattctgatt cgccagtgtc attcgtctga tgtgacagtg agtacagcca ggcgttgtcc
    14641 tttgccgtga tatgcgcggt actgcagcgt atgccggtca gggcagcaaa tggtggcgtg
    14701 cgctctgctg atgtccggtc agattcatca acaacacgaa gggagtagcc attgtcgcag
    14761 gccacgttaa tcagttcagc gagagtaaca ccatcgacgt caacgacgat tcgccccatg
    14821 ttcagtgcct gcacgttaat actgtctgct tccagcgtca gggccagttt cattgtttcg
    14881 cctccggatg cttacccagg gtaatgttat ttaccgttct gtaattgtcg cgggtcatca
    14941 ggcctgtcgc ccggcgggcc cggaggatat cgatgctgtt tattaactga gagtggttac
    15001 aggtgctgaa tcccggctgg tcggtacgta ccagcgcgta tttttcaacg agaaagttca
    15061 ccgcatcaca cagtgaaatg cctgcctcaa tatgctgctc aatcacacgt tcatcagcga
    15121 acggtgtgtc attcagcgtg aggccgtagt gctggtccag caaccgggcc agcagtatct
    15181 gccagatttc aaccggagac gggcgtgaac tggtcgtctg cccgggtaat acaggtaatg
    15241 ttttcatact gaagattttc ctgatatgca gagatgaaaa atgggaaagt ggcgtggtga
    15301 aaacaccagg ccggagcaga aggttattct gatgatttac tttttcgttt cgggtgtcgt
    15361 ataaacagcc agataaacgt aaccacagct gccgagggtg tcggcttcgc aggtcagccc
    15421 tctggcatac agcgtgacgg tatgctgatg gcggggattc agttcaccgc tggtgagcat
    15481 gagttccagt tgtttcatca gcagcggaaa ggcctggtcc aggtggtatg catctgcatc
    15541 gctgaaccgc cctctgatac cggcacggtc ggcaaggtaa tgcaactggt tgccttcctg
    15601 caccagacgg gcgccaaaac agggtgtcgc ggtgcagggt agcccccacc aggggcggtc
    15661 gtgattgtcg ttggggttat ttgtctcagg gagcgtgtct gacacgataa aatcctcaca
    15721 gaaaatcggt taagaatggt ttaacgatga cgggtcagcc gacgcgcaga ccggcttcag
    15781 tgaaggtgta ttcgttgatg agaagggcct catccacagc ggcgtctgag gtcagccagt
    15841 cgtactcgtt ttccagttga cggtacaacc agaaggccag gtcgcgcagg gcttcgctga
    15901 caacggcttc cgcatcggct gtcattgcct gaccagtcgg actgttccgg gtgaccgtga
    15961 tatccatgct gtacggatgg cagtagcgtc cccggtgact gatttcagcc tgtaactgcc
    16021 agaagttacg cttctgtacc gcctgcaggg tatcagcaat acgatgaagc tcacgctctt
    16081 gcggtgcatg ctccctgatt ctccgggctg ttgcagactg ccagtgccag cgtccttcaa
    16141 aacaggctcc gtccccctgg ctgcagaatc cgctgaacca gatgcacggc tcttccataa
    16201 acctgccgtt gctgagccgg aaaacgcgct ggcggaggtc aatcccgaaa atgtcacaga
    16261 cctccctgaa gtcctcgtac acgttttcat accagttgct gtcagaatgg tgcacacggt
    16321 accagtcgcg ggctttttcc cgtgcagcag ggcaggaaag ttcatccagc gtatacacgg
    16381 tggtggcgat aattctgctc atcagaatct ccttcctgac tcagttcagg gtgacgctca
    16441 tcagccggac atacgggcca aaactgtcct tacggcgttc
//
LOCUS       DAZFFG010000100        16153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFFG010000100 DAZFFG010000000
VERSION     DAZFFG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.100"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            292..1191
                     /gene="yegS"
                     /locus_tag="ACHJ7J_003705"
     CDS             292..1191
                     /gene="yegS"
                     /locus_tag="ACHJ7J_003705"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO5001886.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIHGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEENPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1273..2046)
                     /locus_tag="ACHJ7J_003706"
     CDS             complement(1273..2046)
                     /locus_tag="ACHJ7J_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5001887.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2152..3192)
                     /gene="gatD"
                     /locus_tag="ACHJ7J_003707"
     CDS             complement(2152..3192)
                     /gene="gatD"
                     /locus_tag="ACHJ7J_003707"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5001888.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3240..4595)
                     /locus_tag="ACHJ7J_003708"
     CDS             complement(3240..4595)
                     /locus_tag="ACHJ7J_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO5001889.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILADYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIILSITLWISTQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(4599..4883)
                     /gene="gatB"
                     /locus_tag="ACHJ7J_003709"
     CDS             complement(4599..4883)
                     /gene="gatB"
                     /locus_tag="ACHJ7J_003709"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO5001890.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4914..5366)
                     /gene="gatA"
                     /locus_tag="ACHJ7J_003710"
     CDS             complement(4914..5366)
                     /gene="gatA"
                     /locus_tag="ACHJ7J_003710"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO5001891.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5376..6638)
                     /gene="gatZ"
                     /locus_tag="ACHJ7J_003711"
     CDS             complement(5376..6638)
                     /gene="gatZ"
                     /locus_tag="ACHJ7J_003711"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO5001892.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYRELVRDHFA
                     ILKVGPALTFALREAVFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRTYRYGCAE"
     gene            complement(6667..7521)
                     /gene="gatY"
                     /locus_tag="ACHJ7J_003712"
     CDS             complement(6667..7521)
                     /gene="gatY"
                     /locus_tag="ACHJ7J_003712"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO5001893.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7821..8873)
                     /gene="fbaB"
                     /locus_tag="ACHJ7J_003713"
     CDS             complement(7821..8873)
                     /gene="fbaB"
                     /locus_tag="ACHJ7J_003713"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO5001894.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9130..10407
                     /locus_tag="ACHJ7J_003714"
     CDS             9130..10407
                     /locus_tag="ACHJ7J_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO5001895.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLITAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10404..11408
                     /locus_tag="ACHJ7J_003715"
     CDS             10404..11408
                     /locus_tag="ACHJ7J_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO5001896.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSVIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11405..12370
                     /locus_tag="ACHJ7J_003716"
     CDS             11405..12370
                     /locus_tag="ACHJ7J_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5001897.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASACIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12344..13090)
                     /locus_tag="ACHJ7J_003717"
     CDS             complement(12344..13090)
                     /locus_tag="ACHJ7J_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5001898.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13142..13960)
                     /locus_tag="ACHJ7J_003718"
     CDS             complement(13142..13960)
                     /locus_tag="ACHJ7J_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO5001899.1"
                     /translation="MQLSITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(14025..14825)
                     /gene="thiD"
                     /locus_tag="ACHJ7J_003719"
     CDS             complement(14025..14825)
                     /gene="thiD"
                     /locus_tag="ACHJ7J_003719"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO5001900.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14822..15610)
                     /gene="thiM"
                     /locus_tag="ACHJ7J_003720"
     CDS             complement(14822..15610)
                     /gene="thiM"
                     /locus_tag="ACHJ7J_003720"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO5001901.1"
                     /translation="MQVDLLSFAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVATGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPIMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15645..15741)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15833..16105)
                     /gene="rcnR"
                     /locus_tag="ACHJ7J_003721"
     CDS             complement(15833..16105)
                     /gene="rcnR"
                     /locus_tag="ACHJ7J_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO5001902.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         3979 a         4211 c         3901 g         4062 t
ORIGIN      
        1 taagtaggaa ttacggacat ggaggccctt ccgggtaact ccatgggggc tttaatatat
       61 tatattgaag atgccactgt ttaattgaat attaggtata tgctcttttt tgaaatttat
      121 cggtggctgc cgttagtatt cgctgtcccc attgcaagct cctggtggta accactgaat
      181 cctccatact tgaactgact ttttatcctc cgactttcat cctgttctga ctccaccttt
      241 tcttttctgc tctacactat ctacagacca atcataaagg cacatacgat catggcagaa
      301 tttcccgcca gcttactgat tcttaatggc aaaagtactg acaatctacc cttgcgcgaa
      361 gcaattatgc tgttgcgtga ggaaggaatg acgatccatg tgcgggtcac ctgggagaaa
      421 ggcgatgccg cacgatatgt agaggaggcc cggaagttgg gcgtcgcaac ggtgattgcc
      481 ggtggtggcg atggcaccat taatgaagtt tctacggcgt tgattcagtg tgagggggat
      541 gacatacccg cgctgggaat tttgccatta ggaaccgcca atgattttgc caccagtgta
      601 gggattcctg aggcactgga taaggcgctg aaactggcaa ttgccggtaa cgccattgcg
      661 atagatatgg cgcaggtcaa caaacaaacc tgttttatta atatggcgac aggcggattt
      721 gggacgcgta ttaccacaga aacgccggaa aaattaaaag ccgcgctggg tggcgtctct
      781 tatatcattc atggcttaat gcgcatggat actctgcaac cggaccgttg tgaaatccac
      841 ggtgaaaact ttcactggca aggtgacgcc ctggtcattg gtattggtaa cgggcgtcag
      901 gccggtggcg gtcagcaatt gtgtccgaac gcgttaatta acgatggctt gctgcaactg
      961 cgcattttta ccggcgatga aattcttcca gctctcgtat caaccttaaa atctgacgaa
     1021 gaaaacccga atattatcga aggcgcttcg tcgtggtttg atatacaagc cccacacgaa
     1081 atcactttta atcttgatgg cgaaccgttg agtgggcaaa acttccatat tgaaatactt
     1141 ccggcggcgt tgcgttgtcg attaccacca gattgtccat tgttgcgtta atcaaactat
     1201 tctctcttga accagagaga atccgtaatg ccggatgcgg tgtaaacacc ttatccggca
     1261 tacagaacaa tactacaccg taattaattt cacccgtgtc ttctgcaatg ccagtttatc
     1321 cacctctgac acagcatcat cggtaataat aatgtcaatt ttttcgattg gcagtacctg
     1381 attaaaaccg cgacgattaa atttcgatga atcgagaacg gcaatcactt tattagcggc
     1441 ggtgaccatc gcaccgctaa tcgaataacc ttcattaaag gtcgtaatac cattaaccgc
     1501 atcaatacca tcagcaccga caaacattaa atctgcatta atatcctgca atgaacgctc
     1561 ggcaatggaa ccatgcatcg agcgcgtttt atggcggacc gtgccaccac agacgaccag
     1621 agtaatgtct ttattttcgg aaagggcaaa cgctgccggg agactgttgg taatcaccgt
     1681 gatatttttg gcggtcatta atccttcagc gataagcatt gtcgtacttc cgctgtcgag
     1741 aataacggtc atcccttcgt ggatcatttt gactgccgcc tgcgctattc tgttttttgg
     1801 cgcgctggcg agctgaaatc gctctttaaa cccgacttcc tgggtctcgg tttcactatt
     1861 accagacatt attttcgccg caccgccatg aaagcgcgta accacgcctt tttgttcgag
     1921 aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca aatactcccg ccagatcctg
     1981 aacaagcacg gttccctgtt cattcactaa ttggatgatc ttattccttc gctcgaatga
     2041 attcatcgtc atttacccat attcaattgt ggctagtgta aacgaagggg gaccattggt
     2101 gaatgatctg ttttgcacaa taccgggcca gcatcacgct ggcccgcggt ttcagggaat
     2161 gagcaacact ttgcccggca tagcattacg agcgatgtca cgcaccgcct gggtgaagct
     2221 ttcaaagctt ccacggtgag cgattaatgg ctccaggctt aacttacgtt ctgtcagcaa
     2281 ccgactcgcc gtttcccact cctgccccgg ccaagggctg gagtagttca tccaactgcc
     2341 gataaccgtc agctctttac gcaatatttt gccaaacgtt gccgatgtta aatgcagatc
     2401 ctgatgcaac gtgcccacca gcgccagttg ggcatgagga ccggcaatct ctaccgccag
     2461 ttcgacagtt tgcggtacgc cagccgtctc gaggataagc tgattaaagc gcagctcgcg
     2521 taaaacgccc tgcatttgcg gcgcgctcat ttcacggctg ttaaatgttt gcatcgcacc
     2581 gaaagatttt gccagtgcca gtttttctga gctaatgtcg atcgccgtca cactctttgc
     2641 tcccagcgcg acagcgcact gaatagccag caggccaatg gttccggcac caataataat
     2701 aacgttttta ttctcacaac cttgcgctaa atgaaaagca tgcaggccaa cggtaatcgg
     2761 ttcaataaaa gccccatcct caataggcat atccgtgggt agagcaaaga catttttccg
     2821 cttaacgaca atatattcgg caaatccacc atcacgccgt gagccaataa aatcatattt
     2881 tgcgcactgg gaataaaacc ctttcagaca ctctggacaa gtaaaacagg gtaataacgg
     2941 cacacaggca accgcatcgc cagggtgtaa atcatcaaca ccggatccca ccgcatcaat
     3001 atagccgcta aattcatggc ctaacgttat tggataataa tgtgcaccat ttttaaatat
     3061 cctgggtaaa tcggaaccac ataagcccga gctggcaatt tttacccgca cctcatcctg
     3121 atgtttaatt tcaggaatga cgctttctgc aacgcgcacg ataccatcag tatcattcac
     3181 cactgatttc ataaaaactc ctgattgtta agggggatta cctccccctc aggtaaaaat
     3241 tattctgcga gaacggcttt ctcttgttta ataaaaccac gcgctctacg ccaggtcatg
     3301 aaaataccgg tcagataaat tgcgccgata ataatgaaac cgggaatatt ttgcggggag
     3361 aaaacctgaa tcagtaacca ggtaatggga gaaccgccct gatccattga agccaccata
     3421 cccccggctt ttaacgcccc agcattagcc gccagttggg tgtgcaaacc aatagtttgc
     3481 gtcgagatcc acagggtgat gctcaaaatg atgacacccg agattaaggt gcggaacaga
     3541 tttccacgat gcaccgcgac cgccatcgcc acgaagaagc cgatagtggc aagatcgcca
     3601 aacggcagca cctgattacc cggcacacaa acagcaatta aaatggtgag tggaataaaa
     3661 atcaggcttg ccgataccac cgccgtatgc cccagcagta atgcgggatc caggccaatc
     3721 aggaactcct gaccgccgaa cttcgcctgt aaacgactac gggcctgctt agcgatgggc
     3781 gttaaaccat ccatgatggg tttaatcacc cgtggcatca gcagcataac tgccgccgtt
     3841 tttaccgcca gctgcaatac acctttgaca tcgtaatccg cgaggatgcc gataatcagc
     3901 cccatcacaa aacccacggt gacaggctcg ccaaacgggc caaatttgcg ctgaatatcg
     3961 tcggcgctaa atttaatacg gtttacgcct gggattttct cgataatggc atcgaccagc
     4021 accgcaatcg gccccatata cgccgacgta ccgtgcggaa tagcaatgcc ttccagctca
     4081 aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa cgccgcgtga
     4141 attaccactc ccgccatccc tatcatccat gaaccggttg ccagatgcag caacgcgccg
     4201 gtgaaggtca tatgccagat attccagata tcaacattta ccacccgcgt catacgggtc
     4261 agtaacatcg ccacgttaac cagaatcgca atcggaatcg ccaccagcgc aatttgcgac
     4321 gcccaggtca ttggtgaaga gcccggccag ccgacatcga ccacatgcag attcaggtcg
     4381 aaattttccg ccatcgcttt cgccgccggg ccaatggaat ccagcattaa gccaatcaca
     4441 aggccaatgc caacaaagcc aatcccgata tgcagacccg ctttaaagca atcgcctgcc
     4501 ttcatgccta aaattttaga aaaaataatg atgacaatcg gcaacatcac cgtagggccg
     4561 agatcgagaa tataacgcat gacttctgaa aacataggtc acccctgtaa gatagtcaga
     4621 attttatttt gtaatgcttc gataccgaca ccagaaacaa aaggcatgcc gtgaactaat
     4681 ggaatatcgc caaaactacg atccactttg gcagtggtgc atatcaaatg cacgccatcc
     4741 atataggttt ctatttcatt aacccgacac tggattaatt caacaggaat attatgactc
     4801 tgacacaact ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc gcctccgcaa
     4861 gcgacaataa ttttgcgttt cataaatagt cctttttatt gttacagagg gatttattcg
     4921 tctgaatcta aaacatactt tgtgaagtat tcctttaact gagtttcagg aagagtgatt
     4981 agtgtctcaa cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa aagtttcaat
     5041 tgctgctgcg gattttccac aatcaacgca ataaccaacg ataccgccac gtcattatca
     5101 tcatccgctt gctgaaaatg aactttattt gttggcctta acagataaat ggctgacgac
     5161 ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt gctgctcaag cattatccca
     5221 gtagggaatt ctgcttctct ggcaattaat gcctgtggcc aggtatcatg aaccacacct
     5281 ttcgcgagca tctcattacc gatatgggtt aaaacttcgc tacgatcgac aaaagaaatt
     5341 ccgctacgaa caaacaggtt agtcatatac cgtccttatt ccgcacagcc gtagcgatag
     5401 gtgcgcaaaa catcataaat tttatccata ataagctgat gcggtattgc tgataattcc
     5461 ccggactgaa tgcgttcaaa ttgtttagga agatactgac taatcatgcc cagtgggatg
     5521 tccacgcctt ccagattcac catcatcgtt tcgacgctat ttttaatccg actatgcggc
     5581 cagtaataac gaatacgatc cgacaggctg taacgaatat ccagtaatga atcgttaaaa
     5641 cccgtgcgat aatatttttt ccagtattgc ggttcgtcga gcatcacttc ttcaattacc
     5701 gccaggcaac cgctgcgatt ttcgggggcg ataagttcct gctcaatttg tgccagcgca
     5761 aataccgctt cacgtaaagc aaaggttaat gcgggaccga ctttcaatat tgcaaagtga
     5821 tcgcggacta attcccgata agccgtccgg gtctggtaat cggtagaatg tgcttcataa
     5881 accattcggg tattttctat ccattgcgcc agcgcctgcg cttcctgcgg ctgataatgg
     5941 ataatattgc tgtgatcaaa ttccacaccc ggctgcacca cgatggcaat cacgcgtgtt
     6001 aacgcctctg tcagcccacg ggcaataaag gccttttgat gcgtacgtaa agtattggcg
     6061 gcatcttcaa catgggtgat gtgtactgac tgaatggcgc tagcctcacc gcccggaacc
     6121 ggaacttcgg tgccaatgac atagctcagt tgctcacgct ggcaatctgt cgccacactt
     6181 tccgcagcaa agcaaagcac agcagctcgt tccgcaaccg tttctggtgc taacggtatg
     6241 ggatcccccg cgcaggacat tgacgcatca agatgaattt tactgaagcc agcacgaaca
     6301 tatgctttta ccagctcgac ggatttttcc attgccgcat ccgcattttc ttgctgccag
     6361 cagtttggcc ccagatggtc tccgccgaga ataatgcgct cgcgcgcaaa tccgacttta
     6421 tcggcaatcg taaaaacaaa ttcgcgaaag tctgccggtg tcattccggt ataaccgcca
     6481 aattgattga cctggtttga cgttgcttca atcagcactt tgcgcgtgct gttgcgatca
     6541 aatgccagcg ccgcttcgat aaccaacgga tgggcagaac agactgaaca tatgccgata
     6601 tgttcaccag ctttatgccg ggcaattaac gttttcatgt tttttccttg ttaaatggca
     6661 ggtgcgttat gccctgccct cgcagccaca atcggcaatc actttgctca ccacatcgcg
     6721 cattgcggat ttagccgact gcaaataatc ccggggatcg gtcgcttcgg ggtgctcggt
     6781 caggtaattt tttaacgcct gcgagaaggc atttttcagc tccgttgcaa cgttgatttt
     6841 gcatatcccc agtttgatgg tttgctgaat atccttagtc gataaccctg acgcgccatg
     6901 cagcaccagc ggtaagttca cccactggcg aatgttctcc agtcgagaaa aatcaagcgc
     6961 cggtgcgctg gcatacatac catgagccgt gccgatggcg accgccaggg aatcaatgcc
     7021 ggttgcctcg gcaaattcac gcgcctgagc ggggttggta taaaaagcat cggcttcatt
     7081 gacttgcaca tcatcttcct ggccgccaag ttgccccagc tccgcttcga cgctgacatc
     7141 aaagcgatgg caaaaatcca ccacctcttt gacccgtgaa atattttgcg caaaaggcaa
     7201 atgcgaggcg tcaatcatga ctgagcgcac gccagaacga accttctgag cgatatcgtc
     7261 aaatttcgtg tgatggtcga gatgaatcgc cagtggatgg tgataatgct tcgccatcgc
     7321 gctgaccagc gccaacagat tttctgtacc agcatgagta aatgtgccag gcgttccggc
     7381 gatgatgacc ggcgcatgca ggttggcagc ggtttctacc accacttgca tcgtttcgag
     7441 attgtgaata ttgaatgccg gaaccgcgta accgccgcgc tgcgcgttgt tcagcatctg
     7501 ctttgtcgat accacgtaca ttttcaaatc ctgtcgtttg ttttcgattt caaaacataa
     7561 tgaaattatt tgtttttaaa tgtcgagaca acgatcacaa aaacgacaat atgaaaattg
     7621 ttagaggagt gaaaggcaaa aaaaacggcc tcccgatagg gaagccgtag caaagtgcgc
     7681 gttattttat gccggatgca gtgtaaacat acgtggcagc gctgtgtagg catgataaga
     7741 cgcttcagcg tcgcatcagg cataggttgc cggatgcagc gtaaacgcct tatccgtcct
     7801 acggggtagt gccgttcgta tcaggcgata gtaattttgc tatcgagata aacgtcctgc
     7861 acggcgttaa tcagtttcac gccgtcagcc atcgatttct tgaacgcctt acgtccaaga
     7921 atcagcccca ttccgccagc gcgtttgttg ataaccgcag tacgcactgc atcgctgagg
     7981 tcagtttcac cgcctgcagc accgccggag tttatcaacc cggcccggcc catatagcag
     8041 ttagctaact gataacgcac cagatcaatc gggttttcgc tggtcaactt gctgtacacg
     8101 cgatcgtcgg tataaccgta attaatcgct ttatagccgc cgttattttc cgccattttt
     8161 tgtttgacga tatctgcacc tatggttgcc gccagatggt tcgcctgacc agtcaggtcg
     8221 gcggaaacat ggtaatcaac gccatctttc ttaaaggcgg agttacgcaa ataggcccac
     8281 agcactgtca ccatgcccag ctcgtgcgca cgttcaaaag ccgcagaaat ttcttcaatc
     8341 tggcgacgtg actcttccga gccaaaatag atagtcgcac caaccgccac cgcacccatg
     8401 ttgaacgcct gctccacgct ggcatacagc gtttgatcgt aggtgttcgg gtaacttagc
     8461 gtctcgttgt gattaagttt gacgaggaat ggaatgcgat gcgcataacg ccgcgatacc
     8521 gacgccagca cgccgtaagt tgatgccaca cagttacagc ccgcttcgat cgccagttca
     8581 acaatgtttt tcgggtcaaa gtagagcggg ttagcagcaa atgaagctcc ggcagagtgc
     8641 tcaacgccct ggtcaacggg cagaatagaa agatatcctg tgccagccag acgcccggtg
     8701 ttgtacaacg tctgcatatt acgtaacacc gctggcgggc gattattgtc aatcatcacg
     8761 cggtctacgt agtcatgtcc ggggagataa agctggtcag aaggaatagt catacaacgg
     8821 tgctgtaaaa ggttgtcggc gtctttgcca agcaactgcg caatatctgt cattactatg
     8881 ctcccgtaaa ttccgattgg atatcggcta tggattgtcc tggcccgcct tttgcgggca
     8941 atcataatcc tggtcgttac gggcgagttt ttccatcttt tggatgtttt ttcagcgttt
     9001 tctgttggct cgattcatca gaaaaaatgt tagcgcggtc aagttttcac cgcaaaggta
     9061 tttaaaaggt attattaagt ggtattgtca tcgcgtacct tacattacct gtcatgaagg
     9121 aattaaaaga tgaaaacaac agcaaagctg tcgttcatga tgtttgttga atggtttatc
     9181 tggggcgcgt ggtttgtgcc attgtggttg tggttaagta aaagcggttt tagtgccgga
     9241 gaaattggct ggtcgtatgc ctgcacagcc attgcggcga tcctgtcgcc aattctggtt
     9301 ggctccatca ctgaccgctt tttctcggcg caaaaagtgc tggcggtatt gatgttcgca
     9361 ggcgcgctgc tgatgtattt cgccgcgcaa cagaccactt ttgccgggtt cttcccgtta
     9421 ctgctggcct actcgctaac ctatatgccg accattgcgc tgactaacag catcgctttt
     9481 gccaacgtgc cagatgttga gcgtgatttc ccgcgcattc gtgtgatggg cactatcggc
     9541 tggattgcct ccggtctggc atgtggtttc ttgccgcaaa tgctggggta tgccgatatc
     9601 tcaccgacta acatcccact gctgattacc gccggaagtt ctgctctgct cggtgtgttt
     9661 gcgtttttcc tgcccgacac gccaccaaaa agcaccggca aaatggacat taaagtcatg
     9721 ctcggcctgg atgcgctgat cctgctgcgc gataaaaact tcctcgtctt tttcttctgt
     9781 tcattcctgt ttgcgatgcc actggcgttc tattacatct ttgccaacgg ttatctgacc
     9841 gaagttggca tgaaaaacgc caccggctgg atgacgctcg gccagttctc tgaaatcttc
     9901 tttatgctgg cattgccgtt tttcactaaa cgctttggta tcaaaaaggt attattgctt
     9961 ggtctgatca ccgctgcgat ccgctatggc ttctttattt acggtagtgc ggatgaatat
    10021 ttcacctacg cgttactgtt cctcggtatt ttgcttcacg gcgtaagtta cgatttttac
    10081 tacgttaccg cttacatcta tgtcgataaa aaagcccccg tgcatatgcg taccgccgcg
    10141 caggggctga tcacgctctg ctgccagggc ttcggcagtt tgctcggcta tcgtcttggc
    10201 ggtgtgatga tggaaaagat gttcgcttat caggaaccgg taaacggact gactttcaac
    10261 tggtccggga tgtggacttt tggcgcggtg atgattgcca ttatcgccgt gctgttcatg
    10321 atctttttcc gcgaatccga caacgaaatt acggctatca aggtcgatga tcgcgatatt
    10381 gcgttgacac aaggggaagt taaatgaaaa cagaacgtat tctcggtgct ctttatgggc
    10441 aggcgttagg ggatgcgatg gggatgccct ccgagctttg gccacgcagc cgcgttaaag
    10501 cacactttgg ctggattgac cgttttctgc ctggaccaaa ggagaataac gcggcctgtt
    10561 attttaaccg cgccgaattc accgacgata cctcgatggc gctgtgtctg gcggatgcgt
    10621 tactggaacg tgaaggcaag atcgatccgg atctgattgg gcgtaatatt ctcgactggg
    10681 cgctgcgttt cgacgccttt aataaaaacg tactaggtcc gacctcgaag attgcgctta
    10741 acgccattcg cgacggtaaa cccgttgctg aactggaaaa caatggcgtg acaaacggcg
    10801 cagcgatgcg cgtctcgcca ttaggttgtt tgcttccagc gcacgatgtt gattccttta
    10861 ttgatgatgt ggcgctggcg tccagcccga cccataaatc cgatctggcg gttgcaggcg
    10921 cggtagtcat cgcatgggcg atttctcgtg ccattgacgg agaaagctgg tcagtaattg
    10981 ttgattcact gccttcaatt gcgcgacatg cacagcaaaa acgcatcact accttcagcg
    11041 cctcactggc agcacgtctg gagattgcgc tgaaaattgt gcgcaatgcc gacggcactg
    11101 aatccgccag cgaacagctt taccaggtcg ttggcgcagg taccagcact attgagtccg
    11161 ttccgtgcgc cattgcgctg gttgaactgg cacaaaccga cccgaaccgt tgcgccgtcc
    11221 tgtgcgctaa ccttggcggc gacacagaca ccatcggtgc tatggcgacg gcaatctgcg
    11281 gcgcgttgca tggcgttaac gctatcgatc ctgcattaaa ggcggagctg gatgcggtaa
    11341 atcagcttga tttcaaccgc tatgccacag cgctggcgaa atatcgtcaa caacgggagg
    11401 cggtatgagc ggcgctcgat tacacacgct gctgcctgaa ttaaccacgc gtcagccggt
    11461 gatggtcgtc ggcgcggcgg tcattgacgt gatcgccgac gcctacgccc tcccctggcg
    11521 tgggtgcgat atcgaactga aacagcagag cgttaacgtt ggcggctgcg cactgaatat
    11581 tgccgtggcg ttaaagcgcc tcggcatcga agcgggtaat gccttgccgc tcggtcaggg
    11641 cgtgtgggcg gagattattc gcaaccggat ggcgaaagag gggttaatca gcctgatcga
    11701 taacgccgaa ggcgataacg gctggtgtct ggcgctggtt gagccggatg gcgaacgtac
    11761 ttttatgtca ttcagcggtg tggaaaatca gtggaatcgc cagtggctgg cgcgattaac
    11821 cgttgcgcct ggcagcctgc tctatttttc cggttatcaa ctggcctcgc cctgcggcga
    11881 attgttagtg gaatggctgg aagagctgca agacgtgacg ccgtttatcg attttggccc
    11941 acgtattggc gatatcccgg atgcattact ggcgcggatc atggcctgtc gacctttagt
    12001 gtcgctcaat cgtcaagagg ctgagattgc cgccgaacgt tttgctttat ccgcagagat
    12061 aacaacactt ggcaaacaat ggcaggagaa atttgccgcg ccgttgatcg ttcgtctcga
    12121 taaagaaggc gcatggtatt tcagcaacga cgcttctgcc tgcattccgg catttccaac
    12181 gcaagttgtc gacaccattg gggcgggcga cagtcatgcc ggtggcgtac ttgccgggct
    12241 ggcctctggt ctgccactgg cggatgccgt attactgggc aatgcagtgg cgtcgtgggt
    12301 tgtcgggcat cggggcggtg attgtgcgcc aacgcgcgag gaactactcc tcgcacacaa
    12361 aaacgtatag atcgctgcga cagtggctaa tgctgtattc aataggccgt tgctgttggt
    12421 caagcgccac ttgcttgatc accagcaccg gtattttgct atctaactga atatgtgact
    12481 gaaactcggc atctggcatc cgggcgctaa cgcgggaacg tgtacgttgt gggtaaatgt
    12541 gctggctgcg gaaatagtca taaagagaaa ttccgatggc atcaacatca tgaattaaat
    12601 gcgccggaac ccacgattcc tcaatcgata ccgcttcttc atcgacatag cgaatacgct
    12661 taagcaaaaa gacatcactt cccgcttcga ccgccagctg ctgcgcgact tcttcgggac
    12721 atttcacgac acgcttgtta acccatagcg tatcgggctt tttcccgcgc aataccacct
    12781 gctgagaaaa acctcgcgct tctttcaacg agtattcgaa gatgttgttg atctgcgtgc
    12841 cgtaaccacg cgaacgcgtc accacgccct cttcttccag cgcctgcatc gccttgcgca
    12901 ccgtaatgcg cgaaacgccg gttaactgac ttaaatcacg ctcaccgggc aaaatattgc
    12961 catgctccag caccccgctg cgcacggcat tttttaccgt ttcggcaaac ttaatataaa
    13021 gcggcgtgtt atccgccgct aaaatacgct cgttcaactg agcgataagc tgggtatgcg
    13081 cttgttccat ttgtcttttt cctgccaggg agtctggtgg gagtatatca ctgcttctta
    13141 tttaaggcgt ttctttaatc ccatcaacga atgcctgcaa ctcctccacc gtgccattaa
    13201 atacattaaa atccaccgga ccattgatgc catctacctg tccacggtcg gaatgctgcc
    13261 agaagcgcca ggccatgccg tcattgtccg gacgacgttg atagtagtgc gccacccacc
    13321 acggatattc attgaagtag cccgccagat tggtgtgata aaaaacggct cctgagtaaa
    13381 taatcggctt tttccccgta cttttttcga ccatttttag ccactgactt acccgcttgc
    13441 gtaattcttt tgccgataat tttccccgtt cttctacgtc cagcacggca gggaaatcgc
    13501 cttgtgagaa atccaccgtt tgcagaaata atctcgcctg aactgaagcg gataccgacg
    13561 gggaaaaata atgatacgcc ccgcgcagca ggccattttc gcggcttagt tgccagttac
    13621 gcgaaaaata ggggtccacc agcttttcgc cttccgtcgc cttaataaag gcaaattgta
    13681 agcgaatacc gttatcacgc atttttgcca cccgctgcca gtcgatccgt tcctgccagc
    13741 gcgaaacatc tattccgtga atggtgtaac tggcaggaat acgaatagcg aaagatttaa
    13801 ccgggcgata accgtagaag tggatgtagt ctgtaatctg aactgctgtc gaatagaaaa
    13861 aattcactgt ctgacgcgga taaattgcaa cgatggaaat tagcccaaga gcgcataata
    13921 aagcggtgaa tttttttcga ctggtaatgc ttagctgcat tggtttcatc cctgaatgtc
    13981 agtgccaaag gctgacaata accaaagcag ctatagtacg gtgctcacca ccaggcgtgg
    14041 aagtggtgaa ccggaccaat accgtgacca acttccagcg tgtcggcctg tgctaacgcc
    14101 gatgaaagcc agctttttgc ctcctgtacg gtgtcagccc agtttgtatg gcgcgggcgt
    14161 agtgcagcca acgccgcaga gagcgtacaa ccagtgccgt gggtgttttt ggtcataatg
    14221 cgcggtgcag taaatcgttg ttctccctca cgagtaaaca gccagtccgg gctttgctca
    14281 tcatccagat gaccaccttt cattagcact gcgccacagc ccatcgccaa cagcgatcgc
    14341 ccttgttcca gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa ggcggctgct
    14401 tcgggcaagt ttggcgttat taatgaaacc tgtggcaata atcgactgcg tagcgtggca
    14461 actgccgaag gtgaaagcag tgggtcgccg ctttttgcca gcataacggt gtcgagtacc
    14521 acgttttgga tctgataacg ttgcaaccgt tctgccaccg cttcaacaat atcggtttcc
    14581 gccaacatac cgattttagt ggtatcgatt cgcacatcgc tgaacaccga atcgagctgg
    14641 gcggcgacaa aatcaggctc aatgcgatac accgactgta cgccacgggt attttgcgcc
    14701 accagtgcag taataactga gcaaccataa gcgccaagtg ccgagaaggt tttaagatcg
    14761 gcctgaatcc ccgcaccacc actcggatca gtaccggcaa tcgtcagagc gttaattcgt
    14821 ttcatgcctg cacctcctgc gtcaattgcc agagcgcatc aaggaaatgt ggaacaaaac
    14881 tgcctggccc ctcgcttctg gcgactgcgc gttctccggc ttgtttcatc cagtgacagg
    14941 cagatgcgac attttccagc atatcgcctg gtaacgcaca acaggcagcg acaaccgccg
    15001 ataatgcaca gccagttcct accactttgg tcattatcgg atcgccaccg tgaatgccaa
    15061 tgatacgatg tccatcggta acataatcca cctcgccagt gaccacgacg attgcgccag
    15121 tttcccgtgc cagtgtttgt gcagcgggta tcgcattagc tgccgcgtca gtggtatcca
    15181 ctccccgtcc gccagtagca acgccagcta atgccatgat ttccgaagca ttaccacgta
    15241 tcgctgccgg tttaaaagat aaaagttcat gacaaaaatg gcggcgataa tcgagcgcac
    15301 caaccgctac tggatcaagc gtccagggtg tttgagagct ttttgcttgc tcaacggcag
    15361 cacgcatcgc ctgtgcgcgt ggctgcgtca gtgtgccaac gttaatcaac aaggcactgg
    15421 cgatagccgc aaactgactg gcctcttcgg tttcgataac catcgctggc gatgcaccga
    15481 tcgccagcaa ggtattggca gtaaaggttt gcaccacatc gttggtcatg cagtgcacaa
    15541 gaggggaatg ttggtgaaaa aggtgtaacg tgtgcgcaga ttgcgcgaaa ctcagcaggt
    15601 cgacttgcat agtttgctcc tgccataacg tgaagaagca atgacctggt ggtccgtgac
    15661 ttccctacgc tggcattatc cagatcaggt gatacgggta tttctcagcc ttcacgcaga
    15721 agggcacccc gagtcgtttg gttgcgatga taaatcgcag aggaggatgg taatgtccag
    15781 cgcacgcgtt gtaaacgagg aaaaaccgca gttttacaat cgcgttaata aattatttga
    15841 tataagaatc cagcaccttc agaacgacat ccagatcttc ttcacgcttt agttcatcgc
    15901 cctggtgaac aatgtgttct gtcagatgac ctttaatcac ttcccgcatc aggccgttta
    15961 ccgcgccacg gatagcagca atctgttgta aaactgcagc gcattcgtgc ggctcgtcga
    16021 gcattttctt gagcgccacg acctggccct gaatcttact ggcacgcgct ttcagtttct
    16081 gtttatcacg gattgtatga gacatggcaa cacctggtta acaagaatat gaaaaatcat
    16141 agcactatta atc
//
LOCUS       DAZFFG010000101        16093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFFG010000101 DAZFFG010000000
VERSION     DAZFFG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.101"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            127..729
                     /gene="ais"
                     /locus_tag="ACHJ7J_003722"
     CDS             127..729
                     /gene="ais"
                     /locus_tag="ACHJ7J_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO5001903.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(768..1193)
                     /gene="nudI"
                     /locus_tag="ACHJ7J_003723"
     CDS             complement(768..1193)
                     /gene="nudI"
                     /locus_tag="ACHJ7J_003723"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO5001904.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALHREIREELGEQLLLTEIKPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            1472..2014
                     /gene="yfaZ"
                     /locus_tag="ACHJ7J_003724"
     CDS             1472..2014
                     /gene="yfaZ"
                     /locus_tag="ACHJ7J_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO5001905.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2114..3316
                     /gene="yfaY"
                     /locus_tag="ACHJ7J_003725"
     CDS             2114..3316
                     /gene="yfaY"
                     /locus_tag="ACHJ7J_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO5001906.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3536..4318
                     /gene="yfaX"
                     /locus_tag="ACHJ7J_003726"
     CDS             3536..4318
                     /gene="yfaX"
                     /locus_tag="ACHJ7J_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO5001907.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4333..5538
                     /gene="rhmD"
                     /locus_tag="ACHJ7J_003727"
     CDS             4333..5538
                     /gene="rhmD"
                     /locus_tag="ACHJ7J_003727"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO5001908.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEIEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5595..6884
                     /gene="yfaV"
                     /locus_tag="ACHJ7J_003728"
     CDS             5595..6884
                     /gene="yfaV"
                     /locus_tag="ACHJ7J_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5001909.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6902..7705
                     /gene="yfaU"
                     /locus_tag="ACHJ7J_003729"
     CDS             6902..7705
                     /gene="yfaU"
                     /locus_tag="ACHJ7J_003729"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO5001910.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7746..7979)
                     /locus_tag="ACHJ7J_003730"
                     /pseudo
     CDS             complement(7746..7979)
                     /locus_tag="ACHJ7J_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7980..8951)
                     /gene="rpnE"
                     /locus_tag="ACHJ7J_003731"
     CDS             complement(7980..8951)
                     /gene="rpnE"
                     /locus_tag="ACHJ7J_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO5001911.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQQGKLEGLQ
                     KGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(9144..10334)
                     /gene="glpC"
                     /locus_tag="ACHJ7J_003732"
     CDS             complement(9144..10334)
                     /gene="glpC"
                     /locus_tag="ACHJ7J_003732"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO5001912.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10331..11590)
                     /gene="glpB"
                     /locus_tag="ACHJ7J_003733"
     CDS             complement(10331..11590)
                     /gene="glpB"
                     /locus_tag="ACHJ7J_003733"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO5001913.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11580..13208)
                     /gene="glpA"
                     /locus_tag="ACHJ7J_003734"
     CDS             complement(11580..13208)
                     /gene="glpA"
                     /locus_tag="ACHJ7J_003734"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO5001914.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQSLHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAQTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13481..14839
                     /gene="glpT"
                     /locus_tag="ACHJ7J_003735"
     CDS             13481..14839
                     /gene="glpT"
                     /locus_tag="ACHJ7J_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO5001915.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            14844..15920
                     /gene="glpQ"
                     /locus_tag="ACHJ7J_003736"
     CDS             14844..15920
                     /gene="glpQ"
                     /locus_tag="ACHJ7J_003736"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5001916.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         3727 a         3951 c         4314 g         4101 t
ORIGIN      
        1 aaattttgag gcattttgcc agcatcgcag gatttttaat attacaccaa ctgctaatta
       61 tcgccagaaa tgtcgctcaa tttcacggta attgtctggt tgcgcttgtc tataggtgga
      121 gtttacgtgt tagctttttg ccgctcttcg ttgaagtcaa aaaaatattt catcatttta
      181 ctggcgctcg ctgcaattgc cggactgggt actcatgccg cctggagtag caatggtttg
      241 ccacgtatcg acaataaaac actggccaga ctggcacagc agcacccggt tgtcgttttg
      301 tttcgccatg ctgaacgttg cgatcgttca accaatcaat gcttgtcaga taaaacaggt
      361 attacggtta aaggtaccca ggatgcccgt gaactgggca acgcttttag cgctgatatc
      421 cctgatttcg atctttattc cagtaatacc gtccggacca ttcagtccgc aacctggttt
      481 tcagcgggta aaaaattgac ggtagataaa cgacttcttc agtgcggtaa tgagatttat
      541 agtgcaatta aggacttaca aagcaaagcg cctgataaaa atatcgttat tttcacccat
      601 aatcattgcc tgacatatat tgctaaagat aagcgtgatg cgacatttaa acctgattat
      661 ctggatggtt tagtcatgca tgtggaaaaa ggcaaagttt atctggatgg ggaattcgtt
      721 aatcactaaa ttttcaatct gacagccagt aatggctgtc atcattgtta cagaagacct
      781 ttcaaacgta acgtttttcg ggtggcgaca ttcaaatcat agtgcgccag atcttcaggt
      841 tttacccacg cgtagtcctg aaactcttcg tttattttca cttctcggtt ggcagaaacg
      901 cagtcaaaaa tcaggtaaat catataaatc tcttccttgc gaccatctgc atacgtcttg
      961 gtgcgaatat catcgctgaa ggtccacggc ttgatttctg tcaaaagcag ctgttctccc
     1021 agttcttcgc gaatttcgcg gtgtagtgcc tcttcaattc gttcgccggg ctccacgccg
     1081 ccacccgaaa gcgcccattg accggggaaa acgccgcgat cgtcggccat tttacacagc
     1141 aaataagcac catcattttg aatcaaaggg catacaatag tccgttgtcg cacaggggta
     1201 ttccttataa ataaatagaa accatgagaa tacggtaact cagaaatatg tgaaagtgac
     1261 agtaatctct tttgcgatga taactgcact tattattatt tttgtgtgta tcggtaaact
     1321 gtgtttattt tttcggcgtt taattattta agaagtattt agttacaata agtgactaga
     1381 tccctttttt cgctcattga atcctctgcg ccaggcagta caatcccgca gctttgggaa
     1441 ttttgattaa ttgcttgtgg gaaaggggat aatgaaaaaa attgcgctag caggtctggc
     1501 cggaatgctg ttggtttctg catcggtcaa tgcaatgagc atcagcggcc aggcgggtaa
     1561 agaatacacc aatattggtg tcggttttgg gactgaatcg acgggcctgg ctttaagcgg
     1621 taactggaca cataacgacg acgacggtga cgtcgcgggc gtggggctgg ggttgaatct
     1681 gcctctcggg ccgttaatgg cgaccgttgg cggaaaaggc gtttacacca accccaatta
     1741 tggcgatgaa ggttatgccg cagcggtagg aggtggtttg cagtggaaaa ttggcaacag
     1801 cttccgtttg tttggcgagt attactactc tccggattca ctctccagcg gtattaaaag
     1861 ttatgaggaa gcgaatgctg gcgcgcgtta caccattatg cgtccagtca gtattgaggc
     1921 gggttatcgc tacctgaatc tgtcgggtaa agacggtaac cgcgacaacg ccgtggctga
     1981 cggcccgtat gttggggtta acgccagttt ctgatccctt ccggcgcggc attctgtcgc
     2041 gccattcagt ttcttactac gccttatctc cctttgcgtt atagtgattc gataacaaaa
     2101 gcaggagata accatgttaa aagtggaaat gctatccacc ggggatgaag tgttacacgg
     2161 gcaaatcgtt gacactaacg ctgcctggct ggccgatttt ttctttcatc aggggttgcc
     2221 attatctcgc cgcaatacgg tgggggataa ccttgatgac ttagtcacca ttcttcgcga
     2281 acgtagtcag cacgccgatg tgctgatcgt taacggcggg ctggggccga ccagtgatga
     2341 tttaagcgca ctcgccgctg cgacagcaaa aggtgaaggc ctggtgctgc atgaagcctg
     2401 gctcaaagag atggaacgct atttccacga acgtggacga gtaatggcac cgagcaaccg
     2461 taaacaagcg gagctgcctg ccagtgctga atttatcaat aacccggtag gcaccgcctg
     2521 tggttttgcc gtgcagctta atcgttgcct gatgttcttt actcccggcg taccgtcaga
     2581 atttaaggtg atggtcgagc acgaaatcct gccgcgtctg cgcgagcgtt tttctttacc
     2641 acagccgccg gtttgtctgc gtttaactac ctttggtcgt tcggaaagcg atctggcaca
     2701 aagcctggac actctacaac tgccgccggg ggtaacaatg ggctatcgct cctcaatgcc
     2761 gatcatcgaa ctgaaactca ccggaccggc aagcgagcaa caggcgatgg aaaaactgtg
     2821 gctggacgtt aaacgtgttg ccggacagag cgtgattttc gaaggcaccg aaggactgcc
     2881 cgcgcagatc agtcgcgaat tacaaaaccg ccagttcagc ctgacgttga gcgagcaatt
     2941 caccggtggt ttattggctt tgcaactttc tcgcgcaggt gctccattgc tggcgtgtga
     3001 agtggttcct tcacaggagg aaaccctggc gcaaactgcg cactggatta cagaacggcg
     3061 ggccaaccat tttgccgggc tggcactggc tgtttcgggt ttcgagaacg agcatctcaa
     3121 ctttgcgcta gccacgccag acggcacttt cgctctgcgt gtgcgtttca gcactacacg
     3181 ctacagcctg gctatccgtc aggaagtgtg cgcaatgatg gcactgaata tgctgcgccg
     3241 ttggttaaac ggccaggaca tcgccagtga gcatggctgg attgaggttg ttgagtccat
     3301 gaccttatcc gtctgattcc accactctgt gccggtttac tgcaccggca cattccccct
     3361 ccggcaaaat ttgtccaacc tgttgtactc aataagaaaa aatccgtgcg ctgattcaca
     3421 aatttgtgcg taatttctcc agtccgttgg acgaggagcg ggatttgatg cgcttcacaa
     3481 ttttactccc cctgagcaac gagactgatt ccagtatcta agactggagt ccagtatgtt
     3541 ggaatcaagc aaagttcccg ccctgacacg ggctattgat attctcaatt tgattgcccg
     3601 cattggcccc tgtaatgctg cgacgatcat tgacacattg gggatcccta aaagcacggc
     3661 ctatttgctg cttaatgaac tcaggcgtca gcgttttctt agccttgatc accaggaaaa
     3721 tttttgtttg tggaccaagc tggtggagct ttccggtcat gcgttaagca agatggatct
     3781 ccgggaactg gctcgaccgc gtttgacgca actgatggat accaccgggc tgttatgtca
     3841 cctggggatt attgataacg gcagcgctta ttacattttg aaagtggagt catcagccac
     3901 tatcagcgtg cgttcacatg aaggaaaaag cctttcgctt tatcgctccg gaataggtaa
     3961 atgcctgctt gcctggcaac ctgcaactgt acagcaaagt attatcgaag ggctggtctg
     4021 ggagcaggcg acacccacaa cgattactca tccacagcaa cttcacgaag aactggcgcg
     4081 tattcgccgt cagggctgga gctatgacaa cggcgaagat tacgccgatg tccgttgtgt
     4141 cgctgcgccg gtgtttaacg ccaataacga gcttaccgct gcgatctcgg tggtcggtac
     4201 ccgcttacaa atcaatgaag aataccgtga ttatctggcg ggcaaggcca ttgcctgcgc
     4261 cagggatatt tcacgtctgt tgggatggaa aagtcccttt gacttacaag cctcataaca
     4321 atggagaaca tcatgaccct accaaaaatt aaacaggttc gcgcctggtt tactggcggt
     4381 gcgacagcag aaaaaggcgc tggcggcggt gattatcacg accagggggc gaatcactgg
     4441 atagacgatc atattgccac cccgatgagt aaataccgcg attatgagca gtcacgccag
     4501 tcatttggca ttaacgttct tggcacgttg attgttgaaa tcgaagcaga aaacggccag
     4561 accggattcg ccgtttcgac agccggtgaa atgggctgtt ttatcgtcga aaaacacctt
     4621 aaccgtttca ttgagggtaa atgtgtcagt gatatcaaac tgatccatga tcaaatgctc
     4681 aatgccaccc tgtattactc cggctctggt ggcctggtga tgaacacgat ttcctgtgtc
     4741 gatctggctc tgtgggatct gttcggcaaa gtggtcgggc ttccggttta taaactttta
     4801 ggcggcgctg ttcgtgatga gattcagttc tacgccacag gtgcgcgtcc ggatctggca
     4861 aaagagatgg gctttatcgg tggcaaaatg ccgacgcact gggggccaca tgatggcgat
     4921 gcggggatcc gcaaagatgc cgctatggtc gcggatatgc gtgaaaaatg cggtgaggat
     4981 ttctggttaa tgctcgactg ctggatgagt caggacgtga actatgcgac caaactggcc
     5041 cacgcttgcg cgccctataa cctgaaatgg atcgaagagt gcctgccgcc acagcagtat
     5101 gaaggttatc gcgaactgaa acgcaacgcg ccagtcggaa tgatggtcac cagcggtgag
     5161 caccacggca cactgcaatc ttttcgtacg ctttcagaaa ccggtatcga cattatgcag
     5221 ccggatgttg gctggtgcgg tggcttaacc acgctggtgg aaattgccgc aatcgccaaa
     5281 tccagggggc aactggtggt gccgcacggt tcgtctgttt actcccacca tgcggtgatc
     5341 accttcacta atacgccatt cagcgaattc ctgatgacca gtccggattg ttcaacgatg
     5401 cgtccgcagt ttgacccgat tctgctcaat gagccggttc cggtgaatgg tcgtattcat
     5461 aaatcagtgc ttgataaacc cggttttggc gtcgaactca atcgtgactg caatttgaaa
     5521 cgcccctaca gccactaatc actccgggcg ttgctgtgac aacggtaacg cccacatatt
     5581 ctaaggagct ttttatgagc accgctttgc ttgacgccgt ggtgaagaaa aaccgtgtgc
     5641 gtttaattcc gtttatgttg gcgctgtatg tgctggcgtt tctcgaccgt tcgaatatcg
     5701 gttttgccaa acagacctac cagattgata ccgggttgag taatgaagct tatgcgctgg
     5761 gagcaggcat tttctttgtg gtatatgcgt ttctgggtgt tccggcgaat cttttgatgc
     5821 gcaaactggg ggccagaacc tggattggta cgacaacact gctgtgggga tttctttcgg
     5881 cagccatggc atgggccgat actgaagcga aatttctgat agttcgcact ctgcttggtg
     5941 ctgcggaggc cggatttttc cctggtatga tttatctcac ctcgcaatgg tttccgcagc
     6001 gtaatcgcgc cagcattatg gggctgttct atatgggcgc accgctggcg ttaacactgg
     6061 gatcaccgct ttccggcgcg ctgctggaga tgcatggatt tatggggcat cccggctggt
     6121 tctggatgtt tgtgattgaa ggattgttgg cagtcggcgc tggggtattc acattctttt
     6181 ggcttgatga cacaccggag caggcacgtt ttctgagtaa acaagaaaaa acgttgctta
     6241 tcaatcaact ggcaagtgaa gaacaacaga aagtgacttc tcggctgagc gatgcgctgc
     6301 gtaatgggcg agtctggcaa ctggcgatta tctacctgac cattcaggtg gcggtttacg
     6361 gattaatttt cttcctgccg acccaggttg cggcattgct gggaacaaaa gtgggcttta
     6421 cagcgtcggt ggtcaccgcc attccgtggg ttgcggcctt gtttgggacc tggcttattc
     6481 cgcgctattc cgataaaacc ggcgaacggc gtaatgtcgc agcgctgaca ttactggcgg
     6541 cgggcattgg tattggtctg tccgggctgc tttctccagt actggcgatc gtagcgctgt
     6601 gtgttgcagc catcgggttt attgccgtgc agccggtgtt ctggacgatg ccgacacaac
     6661 tgctttccgg tacggcgctg gctgcgggga ttggttttgt aaacctgttt ggtgcagtgg
     6721 gcgggtttat tgccccgatc ctgcgcgtga aagcagaaac gttattttcc agcgatgcgg
     6781 cgggattact gacgctggca gcggtggcgg tcatcggttc gctgattatt ttcactctgc
     6841 gtgtaaatcg tactgttgcg cagaccgacg tggcacatca ttaaataggt taaggaacac
     6901 gatgaacgca ttattaacca atccctttaa agaacgttta cgcaagggcg aagtgcaaat
     6961 tggtctgtgg ttaagctcaa caactgccta tatggcagaa attgccgcca cttccggtta
     7021 tgactggttg ctgattgatg gggagcacgc gccaaacacc attcaggatc tttatcacca
     7081 gctacaggcg gtagccccct atgccagcca acccgtgatc cgtccggtgg aaggcagtaa
     7141 accgctgatt aaacaagtcc tggatattgg cgcgcaaact ctactgatcc cgatggtcga
     7201 tactgccgat caggcacgtc aggtggtgtc tgccacgcgc tatcctccct acggtgaacg
     7261 tggtgttggg gccagtgtgg cacgggctgc gcgctgggga cgcattgaga attacatggc
     7321 gcaagtcaac gattcgcttt gtctgttagt gcaggtggaa agtaaaacgg cactggataa
     7381 cctggacgag atcctcgacg tcgaagggat tgatggcgtg tttattggac ctgcggatct
     7441 ttctgcgtcg ttgggctacc cggataacgc cgggcacccg gaagtgcagc gaattattga
     7501 aaccagcatt cggcggatcc gtgctgcggg taaagcggct ggttttctgg ctgtggctcc
     7561 tgatatggcg cagcaatgcc tggcgtgggg agcgaacttt gtcgctgttg gcgttgacac
     7621 gatgctctac agcgatgccc tggatcaacg actggcgatg tttaaatcag gcaaaaatgg
     7681 gccacgcata aaaggtagtt attgatatca aaggcccatg gggatcggct gtgggcctgt
     7741 gttaattagt ggtttttcgc tgccagatcg gcttcgctta gctgggtggc cgcgagcacc
     7801 aggtcacgat caatgccctg ttccagcatg cgcaaggcaa ttttaatagc ttgttcatgc
     7861 atgccttcct gcaaaccttc taatttacct tgctgcaaac ctactaattt accttcttgc
     7921 cagccaattt gatgtccttc ctgctgtagc cgttcagcaa tagtcatcaa tctctccttt
     7981 taatgacagt tcggaatatc agcatcagtg agttgggttg ccgccagcac aatctcacgt
     8041 tcaaagccct gctccagcat gcgcaaggca atttttatgg cttgctcatg catgccttcc
     8101 tgcaaacctt ctaatttacc tttctgcaga ccctccaatt taccttgctg caaaccttct
     8161 aatttacctt cctgccaacc aatttgatgt ccttcctgcc gtagccgttc agcaatagtc
     8221 attaatctct ccttttgtag tggtgaacgt tcggcaattt cctgaataaa acgggtgaaa
     8281 cgggaggtat cgccgcattg cagcagataa ttaaacagtg tttgtagctg gctgtcatta
     8341 gtgaagcctc taaccaaaag cgtggtgatc ctgtcgacca taccgattaa atcgcggtcg
     8401 cgaatatgct tttgaatcag ttcaagcaga gctatacgcc gatgttgcat gatctcatcg
     8461 tcaggtacga tggtaatatc caccaacgga aaagcttcgg tgtacaactg ccgggcaagt
     8521 tgcggatcgt caaactcatc cagccagttg agtgagtacg ggtagggcga ggtttcgcca
     8581 tgataaaaca gcaacggcac caccagcggg actctgtcat agcctttgtc caggtgacgc
     8641 tgcatggcgg cagtggcata gcgcattagc cgaaaagcca tattcttttc tgcagagctt
     8701 tgatgttcaa tcacgcagta gatatagccg tcaccgtcgc tggtttccac ggaccacaaa
     8761 acatccgagt agtaagcgcg taaacttttt tcaataaaac tagtgggttc caggcgtaag
     8821 gtttgcaggt tgcaaagctt gcgcagtggt tctggtaaat gtatttcgag aaaatcccgt
     8881 gcggtttcgg gtgtgaacat aaaggtttta aatactgcat catgcgggga ggaggttgtt
     8941 gattcggtca tggttgtccg tcacctgata tttatgatga cgcgacatta cggttatccc
     9001 aggcctttga catctgccag tttcagcctt tgcgagacgt tttcagagga attttcactg
     9061 cgttgcaacg catacattgg gcacggaatc gtgtacgcaa aaatgcccgg taaccacatg
     9121 cttaccgggc atcagaaagg agtttaagcc agcgcctggg ccagtagcgt aatcggatgt
     9181 tcgcagcgaa gacttgtgga catctcaatc tgccatttac aggtttcgca gtcggtgacc
     9241 accagatctg cgccgctttc ttctatctgg cggaacagtg gtgcgccgat ggcttgtgag
     9301 gtggggtagt tctctttttt gaaaccgtaa gtacccgcaa taccgcagca ctgggaatcc
     9361 agcaccgtta actcaagccc cgggatgtta cgcaacagct ccagggtgta gagcgtccag
     9421 cccatttttt ccatatggca cggagtgtga taaaccactt tcagcggcag cggtttcagc
     9481 ggtaacgttt tgccttcgtc cagcttgcgc cacagccagc gggtcgccag ttcgatatga
     9541 tcgcgcaagc ctttgttgtc gacattcagc acttccgggt attcgtcgcg cagggcaaat
     9601 gtacaggttg aggaggtggc aatcaccgga atgcctttta ctcccacagc ttcgcggatc
     9661 gactctacat tcgtaattgc ctgtttgcgt gctttatcgg taaagccgtt ggcgatcagc
     9721 ggtacgccgc agcatttttc tttgctgagc agttgtacac cggtacccat tgcgttgagc
     9781 actttaatta aatctttacc taactgcgga tggttgtagt taacgaagca gccgtgaaag
     9841 aaagcgacct ggtctttata ctgcgcttgc tgagccgcca cgctgcgata ccagcgacga
     9901 aacgtgccga aggagtattt cggtagcgtg cggcgatgat cgatttttaa cgccgcatca
     9961 agcaactgcc gcaccggttt cagcgaggta gcggtgttga cgattggtgc gaacggcgtg
    10021 gaaacgctac ccatcaggtc ggtatggctt aaaacaaaat tgcgcagcga cgggcgcgtg
    10081 gtgtcatatt tcgcccgtgc gcgctggatg atatcgccaa tcttcacatc ggacgggcag
    10141 gcgacttcgc aacgtttgca gttgatgcaa tatttcagtg cctcgtcata cagtgcgcca
    10201 tcttttaaac gcagacgctc gccatccggc ccggcttgtt ttggccctgg ataaccggga
    10261 ttcacccggc tcactgggca ggcggtggtg cagacggtgc acttaatgca gttttcgaag
    10321 ctggtgtcat tcattgttgg cctcctgcgc gttgggcaat ctgttgagcg gcatgtaaag
    10381 cactgacggc acaaacaccg ccgccgcatc cctgggcgat gggatcaaat ccgcccagca
    10441 ccgaaccgat ggcaaacagg ttttcgatgg tttgccctgc ctgcgacggg cgtagcgtct
    10501 gatcagtggt tacaccgaac tgctgccacg gttgcggcgc aaaaaaatct cccttatacc
    10561 attcaccccg cgtggcggtt tgtagcacat caaggccgag aatcggctct cgaatgccgt
    10621 tacgttccgc taccagtccg ccactaaaga aactgccgct ggcgagaacc gcgaaacgtg
    10681 gacgcagcgg gatatcggcg tgattgcggg tccagatttc attcactacg ccatttttac
    10741 aggtcacttt tttcacttca tcgcccggca tccacacgcc accctgacgc acaaactggc
    10801 gctgtaactg gttttgcaga cgaatgccca gcacggaagg cggcagcgtt ggcaaaagca
    10861 tcagtgaaca aggtagtttt tcattcaacc aacgccacag tttgtcatca gccaggccga
    10921 agcaggcggg catcaggatc atttcgcagg tattggcgac aggaataagc gcatcaagta
    10981 acagcggcca gttttcttca ttatcaagga aacgggcgat attcaccgcg cggaactcgg
    11041 tggcgttatt gcgcaggaca tccagttccg gcagctctat ttctgcggtt tcaacggcaa
    11101 ggccgagttc acgcaacgaa gctgccgcaa ggtgcgcctg aaaatccatc agtccactaa
    11161 ttcccactac acatattttc ttcgcgggca gcggccagac ggggacttct ggcgaactta
    11221 gccaggtaga gcgcagagtg ccgagcggcg taatccgctg gtgagccagt tctacgctgc
    11281 cctgcaattg cgcaccgctt tcagcgatta atgcctgcgc ctggcaagcg agatcgagca
    11341 cgcgttgtgg cccgagaaga gagtaaggat gtgcaggtgc ctgctgacgc aaagattcca
    11401 gtccactgtg aatgtctgtc accggttgac catctggcag atggctcagc aaatccagcg
    11461 aaccggatga gaaatgcagc gcgctttgac cacgcgtgac aatggcacag cgcaggccgt
    11521 gtttttgcag ttgcaggcca cagagtaatc cggcgaggcc gccgcccata atgacagtat
    11581 caaagcgcat ctttctgctc cttctccaga ccacacaatc cctgataaac ccagcgggta
    11641 aattcgcttt cgcgcagtgc atctccccag gcgatgggtt gcacgccttt ccagcgttcg
    11701 ttaaggaagg tggaaagttg ctcgatagat tgcgcggacg tcgtgacgtt aaaacgttgc
    11761 agcagtccgg cagcgcggca ggcgcagagt tcgccctggc aggtacccat ccccacacgg
    11821 gtacgacggc gtaaatccag caggctatta acgtttaaat tttctaccgc gtactgcact
    11881 tcacccgcag ttaccgcttc gcactcacat accaggctac ggtgcagacg gccttcgctc
    11941 agccaggcag gcgtacgatc gccatgacga taaaccgcag aaccgcgcag cggggcaggc
    12001 agggagatga ctttacgcaa ggtaacttca gcgggttcct gtgaacccgg aagtgccaga
    12061 tcggcggtcg tacaggggcg cgtgttgccc agtttgcggc atacggcgtc ggtagcccat
    12121 tccgccatca gccgataggt catcagtttg ccaccggtaa tggtgataaa tccgtccaga
    12181 ccatcgcgtt cagcatgatc gagcagcacg atgccgcggc tgacgttacg cccgctcgga
    12241 tcatcatcgc tggcgaccag tggacgcacg ccagagtagg cccgcaaaat gcgcgtttgc
    12301 gccatcacgg gggccagttt ttccccttca cgcagcagga tatccacttc ttctgccgtc
    12361 acccgattat cgtcaatctc gttgtaatca atacgtaaag aggtggtgcc aatcagcgaa
    12421 atggtatcgc caggcaccag aatatcggcg tcggaaggtt tacggcagcg gttgatcaca
    12481 tgctggttaa tgcggtgatc catgatcagc agcgatcctt tcgccgggaa catgcgaatg
    12541 cgcagatcgg catattcggc aatgtgttgc ccccagatcc cggcagcgtt aaccacgaca
    12601 ggcgcatgta gcgactgggt ttcgccggtg aggtggttgc gtacatgaac gccgcacacc
    12661 gttgcgcctt cacgaatcag tcccgtgact tcatgagcag taaggataac ggcaccgtgt
    12721 tctttggcat ccagcatgtt tgctgcggtc agacgaaatg gatcaacggt gccatccgga
    12781 actttcaccg cgccaatcag tgccgggtta acggcaggtt cgataatgcg cgcttgctgc
    12841 gggtctatag cttctgcgct gatccctgct tcttcgcagg cgcgaataaa agtggcctgg
    12901 aaggagaggt catcttccgg cagggtgata aacaggccgt tggttggttc aacgcagtga
    12961 cgtgcaatgc gtttcaggat ctggttttca ctaatgcatt cgcgggccga ttccgcatcg
    13021 gttaccgcgt agcgcgcacc gctgtgcagc aggccgtggt tacgcccggt ggcaccggtt
    13081 gcgatgtcgt ggcgctcaac caaaatcacg cgcagcccgc gcagggcaca gtcgcgggca
    13141 atcccggctc ccgttgcgcc gccgccaatg ataatcacgt cacttgattg cgagtcgcga
    13201 gttttcattg ttatccctct gaagttcgtt ttttaccatt tagccatagt aaaaacatga
    13261 attgtttgat ttcgcgcata ttcgctcata attcgaaagt gaaacgtgat ttcatgcgtc
    13321 attttgaaca ttctgtaaat cttatttaat aatgtgtgcg gcaattcaca tttaatttat
    13381 gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttctctcac tgaatcaggc
    13441 tgttaatcat aaataagacc acgggccacg gaggctatca atgttgagta tttttaaacc
    13501 agcgccacac aaagcgcgct tacctgccgc ggagatcgat ccgacttatc gtcgattgcg
    13561 ctggcaaatt ttcctgggga tattctttgg ctatgctgct tactatttgg ttcgtaagaa
    13621 ctttgcgctt gctatgcctt atctggttga gcagggattc tcacgcggtg atttaggttt
    13681 tgccctttcg gggatctcga ttgcttatgg attttcgaaa ttcatcatgg gttcggtatc
    13741 ggatcgctcg aatccgcgcg ttttcctgcc cgcaggtttg attctggcgg cggcagtgat
    13801 gttgtttatg ggctttgtgc catgggcgac gtcgagcatt gcggtgatgt ttgtactgtt
    13861 gttcctctgc ggttggttcc aggggatggg gtggccgcca tgcggtcgta ctatggtgca
    13921 ctggtggtcg cagaaagaac gtggcggcat tgtgtcagtg tggaactgtg cgcacaacgt
    13981 cggtggtggt attccgccgc tgctgttcct gctggggatg gcctggttca atgactggca
    14041 cgcggcgctc tatatgcccg ctttctgcgc cattctggtg gcattattcg cctttgcgat
    14101 gatgcgcgat accccgcaat cctgtggcct gccgccgatc gaagagtaca aaaatgatta
    14161 tccggacgac tataacgaaa aagcggaaca ggagctgacc gcgaagcaaa tcttcatgca
    14221 gtacgtgctg ccgaacaaac tgctgtggta tatcgccatc gccaacgtgt tcgtttatct
    14281 gctgcgttac ggcatcctcg actggtcacc gacttatctg aaagaggtta agcatttcgc
    14341 gctggataaa tcctcctggg cctacttctt ctatgagtat gcgggtattc cgggcaccct
    14401 gctgtgcggc tggatgtcgg ataaagtctt ccgtggcaac cgtggggcaa ccggcgtttt
    14461 ctttatgaca ctggtgacca tcgcgactat cgtttactgg atgaacccgg caggtaaccc
    14521 aaccgtcgat atgatttgta tgattgttat cggcttcctg atctacggcc ctgtgatgct
    14581 gatcggtctg catgcgctgg aactggcacc gaaaaaagcg gcaggaacgg cagcgggctt
    14641 taccggtctg tttggttacc tgggcggttc ggtggcggcg agcgcgattg ttggctacac
    14701 cgtggacttc ttcggctggg atggcggctt tatggtaatg attggcggca gcattctggc
    14761 ggttatcttg ttgattgttg tgatgattgg cgaaaaacgt cgccatcagg aattactgca
    14821 aaaacgcaac ggaggctaat ggcatgaaat tgacgctgaa aaaccttagc atggcgatca
    14881 tgatgagcac tatagtcatg ggaagcagtg caatggcggc ggacagcaac gaaaaaatag
    14941 tcatcgccca tcgcggtgcc agtggatatt tgccggagca tacgctgcca gcaaaagcga
    15001 tggcgtatgc gcagggagcg gattatctgg aacaggattt ggtgatgacc aaagacgacc
    15061 atctggttgt tctgcatgac cattacctcg atcgtgttac tgatgttgcc gatcgtttcc
    15121 cggatcgggc gcgcaaagac ggtcgttact acgcgataga tttcacgctg gatgaaatta
    15181 agtcgttgaa atttaccgaa ggtttcgata ttgaaaacgg taaaaaagtg cagacttatc
    15241 cagggcgttt cccaatgggt aagtccgact tccgggtgca cacctttgaa gaagagattg
    15301 aatttgttca ggggttaaat cactctaccg ggaaaaatat cggtatctat ccagaaatca
    15361 aagcgccgtg gttccatcat caggaaggga aggatattgc ggcaaaaacg ctggaagtgc
    15421 tgaagaaata tggttacacc ggtaaagacg ataaagttta tttgcaatgt tttgatgctg
    15481 atgagctgaa gcgtattaag aatgagctgg aacccaaaat gggcatggat ctcaatctgg
    15541 tacagctgat tgcctatacc gactggaatg aaacgcagca gaaacagccg gacggaagct
    15601 gggttaatta caactacgac tggatgttta agccgggtgc catgaaacag gtggcggaat
    15661 atgcagatgg tattggtccg gattaccata tgttgattga ggagacatcg cagccgggta
    15721 atatcaaact cactggcatg gtgcaagatg ctcagcagaa caagctggta gtgcatcctt
    15781 ataccgtgcg gtcagataaa ctgcctgaat acacaactga tgtgaatcag ttatatgatg
    15841 ctctgtataa caaagcgggt gtaaatgggt tgtttactga tttccctgat aaagcggtta
    15901 aattccttaa taaagagtaa tgctgctact gcaggatggc gttcatgcgc catcctctgc
    15961 taaagcgaaa taatgctact accggttatt cttttcatta accggtgcat cctgtaaaaa
    16021 agatgccaac ataattttcg taaaactcat tcgttaaaaa agctaacact gggcattgtt
    16081 attctttaaa cag
//
LOCUS       DAZFFG010000102        15761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFFG010000102 DAZFFG010000000
VERSION     DAZFFG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.102"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1113
                     /locus_tag="ACHJ7J_003737"
     CDS             <1..1113
                     /locus_tag="ACHJ7J_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO5001917.1"
                     /translation="YFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYT
                     NAYRGPMETGKIALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQH
                     FYLNVDRVALSASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIV
                     TETRAAILVFPFFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDL
                     NNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGAL
                     PYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGL
                     SDVILFSKPVPTAVFVTIILLCAYFKAQSDQCLLEK"
     gene            complement(1183..2154)
                     /gene="rfaC"
                     /locus_tag="ACHJ7J_003738"
     CDS             complement(1183..2154)
                     /gene="rfaC"
                     /locus_tag="ACHJ7J_003738"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO5001918.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAEYYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(2158..3204)
                     /gene="rfaF"
                     /locus_tag="ACHJ7J_003739"
     CDS             complement(2158..3204)
                     /gene="rfaF"
                     /locus_tag="ACHJ7J_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO5001919.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(3214..4146)
                     /gene="rfaD"
                     /locus_tag="ACHJ7J_003740"
     CDS             complement(3214..4146)
                     /gene="rfaD"
                     /locus_tag="ACHJ7J_003740"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO5001920.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4360..5556
                     /gene="kbl"
                     /locus_tag="ACHJ7J_003741"
     CDS             4360..5556
                     /gene="kbl"
                     /locus_tag="ACHJ7J_003741"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO5001921.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            5566..6591
                     /gene="tdh"
                     /locus_tag="ACHJ7J_003742"
     CDS             5566..6591
                     /gene="tdh"
                     /locus_tag="ACHJ7J_003742"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO5001922.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFAIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            6830..7864
                     /gene="waaH"
                     /locus_tag="ACHJ7J_003743"
     CDS             6830..7864
                     /gene="waaH"
                     /locus_tag="ACHJ7J_003743"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO5001923.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(7851..8810)
                     /locus_tag="ACHJ7J_003744"
     CDS             complement(7851..8810)
                     /locus_tag="ACHJ7J_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO5001924.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPNAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTQPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(8814..10073)
                     /gene="envC"
                     /locus_tag="ACHJ7J_003745"
     CDS             complement(8814..10073)
                     /gene="envC"
                     /locus_tag="ACHJ7J_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO5001925.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(10107..11651)
                     /gene="gpmM"
                     /locus_tag="ACHJ7J_003746"
                     /gene_synonym="pgmI"
     CDS             complement(10107..11651)
                     /gene="gpmM"
                     /locus_tag="ACHJ7J_003746"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO5001926.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKNRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQSDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVDEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            11896..12327
                     /locus_tag="ACHJ7J_003747"
     CDS             11896..12327
                     /locus_tag="ACHJ7J_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO5001927.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12468..12719
                     /gene="grxC"
                     /locus_tag="ACHJ7J_003748"
     CDS             12468..12719
                     /gene="grxC"
                     /locus_tag="ACHJ7J_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO5001928.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            12782..13249
                     /gene="secB"
                     /locus_tag="ACHJ7J_003749"
     CDS             12782..13249
                     /gene="secB"
                     /locus_tag="ACHJ7J_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO5001929.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            13249..14268
                     /gene="gpsA"
                     /locus_tag="ACHJ7J_003750"
     CDS             13249..14268
                     /gene="gpsA"
                     /locus_tag="ACHJ7J_003750"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO5001930.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            14348..15169
                     /gene="cysE"
                     /locus_tag="ACHJ7J_003751"
     CDS             14348..15169
                     /gene="cysE"
                     /locus_tag="ACHJ7J_003751"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO5001931.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(15222..15695)
                     /gene="trmL"
                     /locus_tag="ACHJ7J_003752"
     CDS             complement(15222..15695)
                     /gene="trmL"
                     /locus_tag="ACHJ7J_003752"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO5001932.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYSAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
BASE COUNT         3674 a         3988 c         4044 g         4055 t
ORIGIN      
        1 tattttcttg cgacaccaaa aaaaacaata actaataatc ctactttgtt aattttcatc
       61 tcattatgtc ttttgggtat cgtaaatatc atctggtatt cacattataa agtttcaggc
      121 tctgtttata ccaatgcata tcgtggccca atggaaactg gaaaaattgc cttgtgtagc
      181 gcttttattt tcttagttct ttttgctaaa aatgagatga gaacaaaaat aaaatttggg
      241 aaactaattc tgttcgcatc cctggcaacg cagttacttt tttttgcgca tgccatgtgg
      301 caacatttct atttaaacgt cgaccgtgtt gcattatcag cttcccacgc tacaacagca
      361 ggctacatca tcctttttcc ttctttactg gcatcaattc tcattttaaa atccgacttt
      421 agacataaaa caacattata tacaattaac ttcatgctta gcttatgtgc tgtcatagta
      481 actgagacgc gtgcagccat attagtgttt ccattctttg cgttaatatt aatcgtaatg
      541 gatagttata ttaataagcg aattaattat aagttatatt gttttattgc gattgcatta
      601 ttagcaggtg tattttcttt taaagataca ttgcttatga gaatgaatga cttaaataac
      661 gatttagtta attattcgca tgataacacc agaacttcag tcggtgcccg tctggcaatg
      721 tatgaagttg gcttaaaaac atattctcca ataggacaat cactggaaaa acgtgcggaa
      781 aaaatacatg aactagaaga aaaagagcct agattgagtg gcgctttacc ctatgtagat
      841 tctcatttgc ataacgatct catagatacg ttatcaacgc gtggtattcc tggagttgta
      901 ttaacaattt tagccttttc agcaatactc atatatgcct taagaactgc taaagaacct
      961 tatattttaa tcttgctttt ttcactactg gtagtaggcc taagtgatgt aatactcttt
     1021 tctaaaccgg ttccgactgc tgtgtttgtc accataatat tgctttgtgc ttattttaaa
     1081 gcacaatcgg accaatgttt attagagaag taatgagtat gtgaaataaa atcagcaagg
     1141 aagctgattt tatttcaaaa ttatctatta aagagttgta atttaaatca tagcagcgtt
     1201 ttcttcaatg aaccgcttaa cagcatttgc tgtcaattga gacaactcat tccccggagc
     1261 cctacatacc atctgattct tcccataccc gccaattaat ccaggatcgg ttggtccata
     1321 aaccgtgata ttgggtctat ccagtgccgc cgttaaatgg cttaaccccg tatccaccga
     1381 cactacaaat ttagccccgg ccagcacgcg ggcaacgcct tccagactca tcttcggcaa
     1441 tacttcaaca taagcaaatc cttccgccag tcgtttcgcc cgttcttcct catgcggcgc
     1501 gccccacgga agtttaatcc gtattcctga atcagccagt aaaccaatca attctcgcca
     1561 gtgttcttcc ggccagtgtt tatcatcacg ggtcgtcgca tgaagaaata cggcatattc
     1621 gccagcatct gtaggcaggt tcgtcaggaa atgctgtgca atagcataat cgccctgggt
     1681 ttgcggtttg ctatagccca ggcttttggc aaacagttcg cgggtgcgtt ctacggcgtg
     1741 ctgctgtttt gcaatatgat gcttacgatt gtaaaacaga ctggctaaag gttcgcgagc
     1801 ggtttgccag tccattccat gctttacgcc atgcgccaga cgcgtcacca gcgccgcgct
     1861 ttttaccagc ccctgagcgt cgataactgc gtcatagtac tctgcttgta gcgcttcacg
     1921 aaacgctttg cgttccgctt ttatgggggc cgagaaccag gctttacgcc agcgacgtat
     1981 tgccacagga ataactcgct caacggcagc gtgccaggaa ggaatctgtg cgaacccttc
     2041 ttccaccacc cagtcaaatt taatccctgg gattgcctgc tgggcatcag tgagtgcggg
     2101 caacgtatgg agaacatcgc ccatcgacga tgttttaacg atcaaaaccc gcatccgtca
     2161 ggcttcctct tgtaacaata gcgcgttgag ttcttccagt acgcgctggg gagtaatgtc
     2221 gattaagctc tggtgataac cctccgcagc gtcaccttta cgcactttgt gatagccggt
     2281 aatcagacgg atcacgcgcg ctttatggga tagcggcggt gtgaagtccg ggctactcgg
     2341 accatacagg gcaaccagcg gacgattgag cgccgccgca acatgcatca ggccagaatc
     2401 gttagtgaca atggctttac aggctgcaat caggataacc gcttgatcaa gctgtgtttc
     2461 ccccgccagg ttccgacacc atgcctgctg ctcggtattc aaagcggcaa gaatctcatt
     2521 gcccgcttca tgatctttcg cagagccaaa cagaaccacc tgataacctt catcaatcag
     2581 ctgctttgcc agctctgcat agtggtagtg tggccagcgt tttgccggac caaactccgc
     2641 acccgggcaa aaaccaatca tcggacgttc tgatgaaagc gaaaattgat tacaggtata
     2701 tgatttttca ccttcgctca cctgcaattg cggccataac aacggttttg gtagatcttg
     2761 tgcggtacgc atcacgcctt tgtcataggc cagcgcgaca tagcgttcca ccattagcgg
     2821 ccaggcttct ttatcgagca cgcgtacatc attgagtaaa ccgtagcgca tctcgccgcg
     2881 ccagccggtg cgatgaggaa tacccgcgaa gaaaggcact aatgcagatt tgaaggagtt
     2941 gggtaagacg taggcgcggt cgtagcgctt ttcacgcagg ctatgaccca gtttgcggcg
     3001 ttcgccgatt tccagcgctc cgtgaccgag aggcatagga atagcttcgt taacttccgg
     3061 catccgcgat aataatggac ggcaccatgc cggtgccatc acatcgatta tcgcctgggg
     3121 atagcgcgcc tggagcgtgc gatagagact ttgcgacatc atcatgtcgc caacccaaga
     3181 cgggccgatc accagtattt tcatgcagag ctcttatgcg tcgcgattca gccaggccat
     3241 gtattccgtt acaccttcag caacggtttt gaacggtttg tcgtaacccg ccgcgcgcag
     3301 atttgtcaga tctgcctgag tgaacgcctg atagcggcct ttcagtttat ccgggaacgg
     3361 aatgtattcg atctggcctt tcttgtgata agccagcgta gcatcagcta cagcctggaa
     3421 ggattccgca cgaccggtgc cgaggttgaa gatgccggaa acgccatttt ccaggaacca
     3481 caggtttaca tctgccacgt cgcccacata gacgaagtca cgtttgaagt tctcgctacc
     3541 ttcgaacagt tttggcgatt caccgttgtt aagctgggtg ttgagatgga aagcgacgct
     3601 cgccatactg cctttatggc cttcacgcgg tccataaacg ttgaaataac ggaatccaac
     3661 aatctgcgag ttcgcttcag gcaggatttg acgaacatat tcatcaaaca ggaattttga
     3721 gtaaccgtag acgttcaacg gtttttcgta ctcgcgggat tcaataaagt cggaggtgcg
     3781 tccgccgtag gtggccgcgg aagatgcata caggaacggg atttcgcgct ccagacagta
     3841 gtgcagcagc tctttggagt attgatagtt gttatccatc atatacttgc catcccactc
     3901 ggtggtggaa gagcatgcgc cttcgtggaa aatcgcttcg acatcgccga actcttcgcc
     3961 agccataatc tggatcagga agtcttcctt atccatatag tccgcgatat ccagatccac
     4021 caggttcaca aacttggtgc cgtctttcag gttgtccacc accagaatat cggtgatgcc
     4081 tttatcattc agggccttaa cgatgttgct gccgataaag cccgcgccgc cggtaacgat
     4141 gatcataact gtaaccttcg aattatggag tcagagacaa tctcagacac gaatacttct
     4201 atcatatcac tacacggcgt tggcttcagc cattcgccaa aagtgagatt gatggacgtt
     4261 atttatcctg aattttgcag aagtgttaac gcgtcatctc gtcgcgacct ataagtttgg
     4321 gtaatatgtg ctggaatttg ccctgtctgg agaatcgcaa tgcgtggaga attttatcag
     4381 cagttaacca acgatctgga aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt
     4441 attacgtctg cgcagcaagc agatatcact gtggctgatg gaagccacgt cattaacttt
     4501 tgtgccaata actatctcgg gctggcgaat catccggatc tgattgcggc ggcaaaggcg
     4561 ggaatggatt ctcacggttt cggcatggct tcggtgcgtt ttatttgcgg cactcaggac
     4621 agccataaag agcttgaaca aaaactggcg gcattcctgg ggatggaaga tgcgattctc
     4681 tactcttcct gctttgatgc taacggtggc ctgtttgaaa cgctgctggg cgcggaagac
     4741 gccattatct ccgacgcact gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa
     4801 gctaaacgct atcgctatgc caacaacgat atgcaggagc ttgaagctcg tctgaaagaa
     4861 gcgcgtgaag ccggtgcgcg tcatgtgctg attgccaccg atggtgtgtt ctcgatggac
     4921 ggcgtgattg ccaatctgaa gggcgtttgc gatctggcag ataaatacga tgccctggtg
     4981 atggtagacg actcccacgc ggtcggtttt gtcggtgaaa acggtcgtgg ttcccatgaa
     5041 tactgcgatg tgatgggccg ggtcgatatt atcaccggta cgcttggtaa agcgctgggc
     5101 ggggcttctg gtggttatac tgcggcgcgc aaagaagtgg ttgagtggct gcgccagcgt
     5161 tctcgtccgt atctgttctc caactcgctg gcaccggcca ttgttgccgc atccatcaaa
     5221 gtactggaga tggtcgaagc gggtagcgag ctgcgtgatc gtctgtgggc gaacgcgcgt
     5281 cagttccgtg agcaaatgtc ggcagcgggc tttaccctgg cgggagccga tcacgccatt
     5341 attccggtca tgcttggtga tgcggtagtg gcgcagaaat ttgcccgtga gctgcaaaaa
     5401 gagggcattt acgtcaccgg tttcttctat ccggtcgttc cgaaaggtca ggcgcgtatt
     5461 cgtacccaga tgtctgcggc gcatacccct gagcaaatta cgcgtgcagt agaagcattc
     5521 acgcgtattg gtaaacaact gggcgttatc gcctgaggat gtgagatgaa agcgttatcc
     5581 aaactgaaag cggaagaggg catctggatg accgacgttc ctgtaccgga actcgggcat
     5641 aacgatctgc tgattaaaat ccgtaaaaca gccatctgcg ggactgacgt tcacatctat
     5701 aactgggatg agtggtcgca aaaaaccatc ccggtgccga tggtcgtggg ccatgaatat
     5761 gtcggtgaag tggtaggtat tggtcaggaa gtgaaaggct tcaagattgg cgatcgcgtt
     5821 tctggcgaag gccatatcac ttgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat
     5881 ctgtgccgca acacgatcgg cgtcggcgtt aaccgtccgg gctgcttcgc cgaatatctg
     5941 gtgatcccgg cgttcaacgc cttcaaaatc ccagacaata tctccgacga cctggcttcc
     6001 atttttgatc ccttcggtaa cgccgtgcat accgcgctgt cgttcgatct ggttggcgag
     6061 gatgtgctgg tctctggtgc aggtccgata ggtattatgg ctgcggcggt ggcgaaacac
     6121 gttggtgcac gcaacgtggt gatcacggat gttaatgaat accgccttga gctggcgcgt
     6181 aaaatgggta tcacccgtgc ggttaacgtc gccaaagaaa atctcaatga tgtgatggca
     6241 gaactgggca tgaccgaagg ctttgatgtc ggtctggaaa tgtccggtgc gccgccagcg
     6301 tttcgtacca tgctcgacac catgaaccac ggcggtcgca ttgcgatgct gggtattcca
     6361 ccttctgaca tgtcgataga ctggacaaaa gtgatcttta agggcttgtt cattaaaggc
     6421 atttacggtc gtgagatgtt tgaaacctgg tacaagatgg cggcgctgat tcagtctggc
     6481 ctcgatctct cgccgatcat tacccatcgt ttcgctatcg atgatttcca gaagggcttt
     6541 gacgctatgc gttcgggcca gtccgggaaa gttattctga gctgggatta acacgaacaa
     6601 gggctggtat tccagccctt ttatctgagg ataatctgtt aaatatgtaa aatcctgtca
     6661 gtgtaataaa gagttcgtaa ttgtgctgat ctcttatata gctgctctca ttatctctct
     6721 accctgaagt gactctctca cctgtaaaaa taatatctca caggcttaat agtttcttaa
     6781 tacaaagcct gtaaaacgtc aggataactt cagaggtcgt cggtaattta tgatgaacag
     6841 caccaataaa cttagtgtta ttattccgtt atataatgcg ggcgatgatt ttcgcacttg
     6901 tatggaatct ttaattacgc aaacctggac tgctctggaa atcattatta ttaacgatgg
     6961 ttcaacggat aattctgttg aaatagcaaa gcattacgca gaaaactatc cgcacgttcg
     7021 tttgttgcat caggcgaatg ctggcgcatc ggtggcgcgt aatcgtggga ttgaagtggc
     7081 aacgggcaaa tatgtcgctt ttgtcgatgc tgacgatgaa gtctatccca ccatgtacga
     7141 aacgctgatg accatggcgt tagaggacga cctcgacgtg gcgcagtgca acgctgactg
     7201 gtgttttcgt gaaacgggag aaacctggca atccatcccc agcgatcgac ttcgctcaac
     7261 cggcgtctta accggcccgg actggctgcg gatggggctt tcttcgcgcc gttggactca
     7321 cgttgtctgg atgggggttt atcgccgcga tgttattgtt aaaaataaca ttaaatttat
     7381 tgccggatta catcatcagg atattgtctg gacaacagaa ttcatgttta atgcgctgcg
     7441 tgcgcgatat accgagcaat cattatataa atattatctg cataatacgt cagtgagtcg
     7501 gttgcataga caagggaata aaaaccttaa ttatcaacgt cactatatta agattacccg
     7561 cctgctggag aaattaaatc gaaattatgc cgacaaaatt acgatttatc cggaatttca
     7621 tcagcaaata acttacgaag cattgcgtgt ttgccatgcg gtgcgcaaag agccggatat
     7681 tcttacccgc caacggatga ttgccgagat atttacttcc ggtatgtata agcgcctgat
     7741 taccaatgtg cgcagcgtga aggtgggtta ccaggcgtta ctgtggtctt tccgcttatg
     7801 gcaatggcgc gacaaaacgc ggtcgcacca tcgcattacg cgtagcgcct ttaatttgcg
     7861 ctagcgttaa gtttcgccgc ttcgggctgt ttgccccagc cttgccactg atgctggaag
     7921 taaacgatca gcgtgctctg gctgatactt tcgcttaata cttcaaagaa gcgattagca
     7981 taaacaggtt ctatcggttt cttcggtttg cacagcttca cgccacggaa cggattacgc
     8041 ggtgtgtcgg gcacagtgtt tttcggctgt gtagacgtat caacctgcgg ttcattcagc
     8101 aggctgctgg ctttcaccag cgtaatgtct ggcggcaggt tataaaccat ctgttgtagc
     8161 acgcgtaccg ttgaagggtg aggatgccca atggcaatgg ttgaaccgtt gcgacgcgcc
     8221 agatcaattg cgcgattgaa ctgcacacgg atgtccgctt cgttctgtga atcgtcgagg
     8281 aacaccttcc gtttaataac cttcacgcca gtgccttgcg cggcgcgcat cgcctgggta
     8341 ttaccaatgg ttacgctgtc gaggaagtaa agattgtaac gctccagcgc ctgcatcact
     8401 ttctgcatac caaacaggtt agaggtcatc ttgctgccca tgtggttgtt gatccccacg
     8461 gcatagggca cgttattgac cgcactacga ataatgcgct caatttcgtc gctgctcatc
     8521 tccgggcgta gcgtattttt ctccagcggc tgtttactca atggtgccat tggtagatga
     8581 atcaacactt cgtggccgct gttatgcgct ttggtcgcca tctctctggc gttcggtgaa
     8641 tcgggtaata cagcgacgga gatagcggaa ggcatcgcca gcacctggtt ttcgttgtgc
     8701 gggcgatacc caaaatcatc aatgacgatg gcaagtttgc cagcaagtac gggggaggag
     8761 agcgccaaca gagcggcaaa tgcaagaacg ttacgacgaa atggaaacaa aacttatctt
     8821 cccaaccacg gctgtggatt gaccgcctga ccctggcggc gaatttcgaa atagagtgaa
     8881 ggccgaccct gaccgccact gctgcccacc agtgcaattg gctggcccgc gcgaacctgc
     8941 gaaccaacgc tcaccagtgc gctctgatta tagccgtaaa gactcatgtc gcctttacca
     9001 tgctcaacca ccaccaccag accgtagcct tgcagccagt cagccagaat cacccgaccg
     9061 tcggcaatcg ctttaacttc agtaccttca gaggcaccaa tcaccatccc tttccagcgt
     9121 aattcaccct gtagctgttc gccatagcga tgcagcgtcg gcccgcgaac cggccagaat
     9181 gcctgaccac gcggcgcgcc cagaccaccg gtacgggaca tcagcgattt ttcgctttca
     9241 gtcggtttgt aggtggtgcc tttacgcgtc gcttctttct ggcggtcgcg aaccgcctgg
     9301 gcctcacgtg cttctcgttc tgcacgcgct ttcgccgcgg cttccgcacg ggcaatgctg
     9361 ttacgcagac gggattcgtt ggcgcgcagc tcgctcaact gttgctgacc ttgctggatg
     9421 gaagactcca gccctgccag cgtcttttta cgctcgttca gcgcctgagt cagtttcgcc
     9481 tgttgggcgc gctgctcata taacagcgtt tgttgctcgc tttgtttctc ttccagttca
     9541 gcacgctgca tggcgacttc ttcacgcgtt tgcttcaact gggcaatggt ttcttgtcgc
     9601 gcctggttga gatagccgaa ataagcctgt aaacgctgtc cacgctggct ttcttcaccg
     9661 ctgagaatca gctgaatacc ggtatgctcg ccctgacgga atgcggcatc cagttgtgcg
     9721 gcgaggctgc gctcctgggc ggctttttgc tgctccagtt tggcaatcga cgcgttcatc
     9781 tcatcaatct gtttattgag ttgattgagc gtgttttgcg tttcgcgcag cttacgggtg
     9841 gcttccgaga tcgcttcttc ctgctttttc aattgtgcga gcaggcttgc gcgttgttgt
     9901 tgcttttggc gtaccgcgcg ctctttcgcg gcgatatcgg cctgaataga tttgagttgg
     9961 tcacgctcat ccgcgtgggc ggaaaaggcg cacaacaata cgccagcgct aagaacgctg
    10021 gcgtagatga tgggcctgat tgcaaacctg cgcggtttca cggcccgtgt catggtatta
    10081 atcgcctttc ccctcatggg gagggattat tccacgatga acagcggctt accagtcatc
    10141 tcttgcggga tttccatacc catcagcgac aacatggtcg gcgcgatgtc agaaagtttg
    10201 ccgccttcaa ccgctttcac gttcttatcg ccaacgtaaa tcagcggaac tggcaggttg
    10261 gtgtgcgctg tgtgcgcctg acccgttgcc ggatcgcgca tctgttcagc gttaccgtgg
    10321 tcagcggtga tcagcagttg tccacctaca gactcaaccg ctttcgcaac ttcatccacg
    10381 cagtgatcca gcgcttcaac ggctttaact gctgcttcca tcaccccggt gtgacctacc
    10441 atgtcaccgt tcgggtagtt acagatgatg gtgtcgtatt tgccgctctt gattgccgca
    10501 accagttttt cggtcagctc tgcggagctc atttccggtt gcagatcgta agttgccact
    10561 ttcggtgagt tgatcagaat gcgatcttcg cctttgaacg actcttctac gccaccgttg
    10621 aagaagaaag taacgtgggc atatttttcg gtttcggaaa ttcgcagctg agttttgtcg
    10681 tttttcgcca tccactcgcc gaaggtgtta accagggaag cgggtgggta agcaaccgca
    10741 gttttgatgt cagcggcgta ttcggtcagc atcacgaaat ccacgttaac cactttctta
    10801 cgcgcgaagc catcgaaatc agcgttcacg aaagcacgag tgatttcacg cgcgcggtca
    10861 gcacggaagt tcatgaaaat cagcgcatcg ccgtcttcca ttgccgcatc tgactgacct
    10921 tctgcacgaa taacggtcgc tttcacgaac tcgtcgtttt cgtcacgagc ataagcagcc
    10981 tgcaaaccag caacggcggt atcggcctgg aactcaccct gcgccagagt cagcaggtca
    11041 taagcttttt caacgcgatc ccaacggtta tcgcggtcca tcgcgtagta acgaccaatg
    11101 atggacgcta cgcggccttt acccagcgcg gcaaatttct cttcgaattt tttcagcgag
    11161 gattcagcac tgcgcggcgg agtgtcgcga ccgtcaagga atgcgtgcag gtagattttt
    11221 tctgcgccgc gttcagctgc cagttctacc atcgccatga tgtgatcttc gtggctgtgt
    11281 acgccgcctg cggagagcag acccataatg tgtaccgctt tgcctgcgtt tttcgcttta
    11341 tctaccgcgc cagtcagcac cggattagca aagaaagcgc gatttttgat ttcaacgtcc
    11401 agacgagtca ggtcctgata cacgatgcgg ccggcaccca ggttaacgtg gcctacttcg
    11461 gagttaccca tctgacggtc aggcagaccg acttccagac cggaagcgtc gatcagggta
    11521 tgcggacgat tggcccacag tgcatccatt accggggttt tagcactaaa aatggcgtta
    11581 tcctgctgtt cttcgcgata gccatagcca tccagaatca ccagtaccat aggtttttta
    11641 gaaaccgaca ttgcgacaac ctcatactca agagtcaaaa tttgcgtaat tttactacag
    11701 ctgaatcgat aaaatagcct ctgaagatca aaatatacga ccgctcgcgg caagccgatg
    11761 ccagatttac gctttttttt cgtcgcgccc cgcaaaattg cattccagtt aacgcgctgg
    11821 ctgtatttgc cgcaccgcgc aggtatactc ctttcctggt ttttttaatc actacgtcgg
    11881 gagttgttac cccccatgca agaaattatg caatttgttg gccgtcatcc catactgagt
    11941 atcgcctgga tcgcgttact ggtggcggtt cttgtgacta cgtttaagag cctgacctcg
    12001 aaagtgaagg tgattactcg tggtgaagct acgcgtctga tcaacaaaga agacgctgtg
    12061 gttgtggatt tacgtcagcg tgatgacttc cgtaaaggcc atatcgcagg ttctattaat
    12121 ctgttgccga gcgaaatcaa agccaacaat gttggtgagc ttgagaagca caaagacaaa
    12181 ccggttatcg tggtagacgg ttctggcatg cagtgccagg agcctgcaaa cgcactgacg
    12241 aaagctggtt ttgcgcaagt attcgtgctg aaagaaggcg tcgctggctg ggctggcgaa
    12301 aacttgcctt tggtgcgcgg caaataattc acctgtcgtc tttcatgatg caggccgtta
    12361 gctgcaatta ggcccccagc gacttattgt tataagcttc tggggacact ccattttgct
    12421 gccgccctgc aacatgaaat ttcagggtaa aaaacaggag ctaagttatg gccaatgttg
    12481 aaatctatac caaagaaacc tgcccgtatt gccatcgtgc aaaagcactg ctgagcagca
    12541 agggcgtgag ttttcaggag ttgccgatcg atggcaacgc cgctaagcgt gaagagatga
    12601 tcaaacgcag cggtcgcacc acggttcccc agatttttat tgacgcacag cacattggcg
    12661 gctgtgatga cttgtatgca ttggatgcac gtggtggact ggatcccctg ctgaaataac
    12721 gtgtgaacgt tggcattaca ttgcgcagta tttaaggaca acacttaagg gttttctaca
    12781 catgtcagaa caaaacaaca ccgaaatgac tttccagatc caacgtattt ataccaagga
    12841 tatttctttc gaagcgccga acgcgccgca cgttttccag aaagattggc agccagaagt
    12901 taaacttgat ctggatacgg catctactca actggcagat gacgtttacg aagtggtact
    12961 gcgtgttacc gtaacggcct ccctgggcga agaaaccgcg ttcctgtgtg aagttcagca
    13021 gggcggtatt ttctccattg cgggtatcga aggcacccag atggcacatt gcctgggcgc
    13081 atactgcccg aacattctgt tcccgtatgc tcgtgagtgc atcaccagca tggtatcccg
    13141 cggtacattc ccgcaactga accttgcgcc ggttaacttc gatgcgctgt tcatgaacta
    13201 tttgcagcag caggctggcg aaggtactga agaacatcag gatgcctgat gaaccaacgt
    13261 aatgcttcaa tgactgtgat cggtgccggc tcgtacggca ccgctcttgc cattaccctg
    13321 gcaagaaatg gccacgaggt tgtcctctgg ggccatgacc ctgaacatat cgcaacgctt
    13381 gaacgcgacc gctgtaacgc cgcgtttctc cccgatgtgc cttttcccga tacactccat
    13441 cttgaaagcg atctcgccac tgcgctggca gccagccgta atattctcgt cgtcgtaccc
    13501 agccatgtct ttggtgaagt gctgcgccag attaaaccgc tgatgcgtcc tgatgcgcgt
    13561 ctggtgtggg cgaccaaagg gctggaagcg gaaaccgggc gtctgttaca ggacgtggcc
    13621 cgcgaggcgt taggcgatca aattccgctg gcggttatct ctggcccaac gtttgcgaaa
    13681 gaactggcgg caggtttacc gacagccatt tcgctggcct cgaccgacca aacttttgct
    13741 gatgatcttc agcagctgct gcactgcggt aaaagtttcc gcgtttacag taacccggat
    13801 ttcattggcg tgcagcttgg cggcgcggtg aaaaacgtca ttgccattgg cgcggggatg
    13861 tccgacggta tcggttttgg cgcgaatgcg cgtacggcgc taatcacccg tggcctggct
    13921 gaaatgtcgc gtcttggcgc ggcgctgggt gccgatcctg ccacctttat gggcatggcg
    13981 gggctgggcg atctggtgct tacctgtacc gacaaccagt cgcgtaaccg ccgttttggc
    14041 atgatgctcg gtcagggcat ggatgtacaa agcgcgcagg agaagattgg tcaggtggtg
    14101 gaaggctacc gcaatacgaa agaagtccgc gaactggcgc atcgcttcgg cgttgaaatg
    14161 ccaataaccg aggaaattta tcaagtatta tattgcggaa aaaacgcgcg cgaggcagca
    14221 ttgacgttat taggtcgtgc acgcaaggac gagcgcagca gccactaacc ccagggaatc
    14281 tttgttaccg ctatgacccg gcccgcgcag aacgggccgg tcattatctc atcgtgtgga
    14341 gtaagcaatg tcgtgtgaag aactggaaat tgtctggaac aatattaaag ccgaagccag
    14401 aacgctggcg gactgtgagc caatgctggc cagtttttac cacgcgacgc tactcaagca
    14461 cgaaaacctt ggcagtgcac tgagctacat gctggcgaac aagctgtcat cgccaattat
    14521 gcctgctatt gctatccgtg aagtggttga agaagcctac gccgctgacc cggaaatgat
    14581 cgcctctgcg gcctgtgata ttcaggcggt gcgtacccgc gacccggcag tcgataaata
    14641 ctcaaccccg ttgttatacc tgaagggttt tcatgccttg caggcctatc gcatcggtca
    14701 ctggttgtgg aatcaggggc gtcgcgcact ggcaatcttt ctgcaaaacc aggtttctgt
    14761 gacgttccag gttgatattc acccggcagc aaaaattggt cgcggtatca tgcttgacca
    14821 cgctacgggt atcgttgtgg gtgaaacggc ggtgattgaa aacgacgtgt cgattctgca
    14881 atccgtcacc cttggcggta ctgggaaaac tagcggcgac cgtcacccga aaattcgtga
    14941 aggcgtgatg attggcgcgg gcgcaaaaat cctcggcaat attgaagttg ggcgcggcgc
    15001 gaagattggc gcaggttccg tggtgctgca accggtgccg ccgcatacca ccgccgctgg
    15061 cgttccggca cgcattgtcg gtaaaccaga cagcgataag ccatcaatgg atatggacca
    15121 gcatttcaac ggtattaacc atacatttga gtatggggat gggatctgat gtcctgtgat
    15181 cgtgccggat gcgatgtaat catctatccg gcatggcgta actaatctct caacaccgct
    15241 cccggatatc ccagctgccg ccaggcttca tacaccacta ccgacaccgc attggacagg
    15301 ttcatgctgc ggctgtccgg caccatcgga atccgaattt tttgttcagc gggcagggca
    15361 tcaagaatgc tcgctggcag gccgcgtgtt tccgggccga acatcagata atcgccatcc
    15421 tgatagctta cggcgctgtg agcaggcgta cctttcgtgg tgagggcaaa caggcgctgg
    15481 ggattttctg cttcgaggaa cgcgctgtag tcatgatgac gcgtaacggc ggtaaactcg
    15541 tgatagtcca gccccgcgcg gcgcaggcgc ttatcgtccc aggcaaatcc catcggttcg
    15601 atgatatgca gacgaaagcc ggtattagcg caaagacgga tgatgttgcc agtatttggc
    15661 ggaatttctg gttcgtaaag tacgatgttt agcatactgc ccccttagtg cgggggcagg
    15721 atagcagaaa attggtgaac cgtgcggcct tttcccctca c
//
LOCUS       DAZFFG010000103        15671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFFG010000103 DAZFFG010000000
VERSION     DAZFFG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.103"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            82..648
                     /gene="yceJ"
                     /locus_tag="ACHJ7J_003753"
     CDS             82..648
                     /gene="yceJ"
                     /locus_tag="ACHJ7J_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5001933.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHE
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            652..1227
                     /gene="yceI"
                     /locus_tag="ACHJ7J_003754"
     CDS             652..1227
                     /gene="yceI"
                     /locus_tag="ACHJ7J_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO5001934.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1269..2321)
                     /gene="trhO"
                     /locus_tag="ACHJ7J_003755"
     CDS             complement(1269..2321)
                     /gene="trhO"
                     /locus_tag="ACHJ7J_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO5001935.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2546..3466
                     /gene="lpxL"
                     /locus_tag="ACHJ7J_003756"
     CDS             2546..3466
                     /gene="lpxL"
                     /locus_tag="ACHJ7J_003756"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO5001936.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3638..4864
                     /gene="mdtG"
                     /locus_tag="ACHJ7J_003757"
     CDS             3638..4864
                     /gene="mdtG"
                     /locus_tag="ACHJ7J_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO5001937.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4947..5321
                     /gene="msyB"
                     /locus_tag="ACHJ7J_003758"
     CDS             4947..5321
                     /gene="msyB"
                     /locus_tag="ACHJ7J_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5001938.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5322..5549)
                     /gene="yceK"
                     /locus_tag="ACHJ7J_003759"
     CDS             complement(5322..5549)
                     /gene="yceK"
                     /locus_tag="ACHJ7J_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5001939.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5722..8265)
                     /gene="mdoH"
                     /locus_tag="ACHJ7J_003760"
     CDS             complement(5722..8265)
                     /gene="mdoH"
                     /locus_tag="ACHJ7J_003760"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO5001940.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8258..9793)
                     /gene="mdoG"
                     /locus_tag="ACHJ7J_003761"
     CDS             complement(8258..9793)
                     /gene="mdoG"
                     /locus_tag="ACHJ7J_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO5001941.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10187..11344
                     /gene="mdoC"
                     /locus_tag="ACHJ7J_003762"
     CDS             10187..11344
                     /gene="mdoC"
                     /locus_tag="ACHJ7J_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO5001942.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHL
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11352..12833)
                     /gene="clsC"
                     /locus_tag="ACHJ7J_003763"
     CDS             complement(11352..12833)
                     /gene="clsC"
                     /locus_tag="ACHJ7J_003763"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO5001943.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12775..13308)
                     /gene="ymdB"
                     /locus_tag="ACHJ7J_003764"
     CDS             complement(12775..13308)
                     /gene="ymdB"
                     /locus_tag="ACHJ7J_003764"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO5001944.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13403..13714)
                     /gene="ymdA"
                     /locus_tag="ACHJ7J_003765"
     CDS             complement(13403..13714)
                     /gene="ymdA"
                     /locus_tag="ACHJ7J_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO5001945.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13835..14167)
                     /gene="csgC"
                     /locus_tag="ACHJ7J_003766"
     CDS             complement(13835..14167)
                     /gene="csgC"
                     /locus_tag="ACHJ7J_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO5001946.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14226..14681)
                     /gene="csgA"
                     /locus_tag="ACHJ7J_003767"
     CDS             complement(14226..14681)
                     /gene="csgA"
                     /locus_tag="ACHJ7J_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO5001947.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14722..15177)
                     /gene="csgB"
                     /locus_tag="ACHJ7J_003768"
     CDS             complement(14722..15177)
                     /gene="csgB"
                     /locus_tag="ACHJ7J_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO5001948.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3917 a         4003 c         3706 g         4045 t
ORIGIN      
        1 ttcaatttgt tatttttgtg ttgcaattca ttctccgaca cgccattttc tttctctcgc
       61 aaattcgttt tggatgcact tatgtcattc acaaataccc ctgaacgcta tggtgttata
      121 tctgcggcct tccattggct aagcgcaatt attgtctacg gcatgtttgc tttaggtctg
      181 tggatggtca cgctcagtta ttacgatggc tggtatcaca aagcacccga actgcataaa
      241 agcatcggta ttttgttaat gatggggctg gttattcgcg ttctgtggcg cgtcatatct
      301 ccaccgccgg gtccgctgcc gagttattcg cccatgactc gtcttgccgc aaaagctgga
      361 catctcgcat tgtacctatt gctgttcgcc atcggcatca gcggctatct gatctcaact
      421 gccgatggta aaccgatcag cgtttttggc tggtttgacg tccccgcgac ccttgccgac
      481 gctggcgcac aggcagactt tgccggtgcc ctgcattttt ggctcgcgtg gagcgtcgta
      541 gtactgtccg ttatgcacga atttatggcc ctgaagcatc atttcatcga taaagacgac
      601 actctgaagc gcatgctggg aaagtcgtca tctgactatg gagtataaaa aatgaaaaaa
      661 agcctgcttg gtttaacctt cgcgtccctg atgttctctg ccggttcagc ggttgccgcc
      721 gattacaaaa ttgacaaaga aggtcagcac gcctttgtta acttccgcat ccagcatctt
      781 ggctatagct ggttatacgg cacctttaaa gatttcgacg gtacttttac ctttgatgaa
      841 aaaaatccgg ctgccgataa agtgaatgtg acaattaaca ccaccagcgt cgatactaac
      901 cacgccgaac gcgataaaca tcttcgcagt gcggattttc tcaataccgc aaaatatcca
      961 caggcaacat tcacctccac cagcgtgaag aaagacggtg acgaactgga tattaccggc
     1021 gatctgacgc tgaatggtgt aaccaaaccc gtcacgctgg aagcgaaatt aattggtcag
     1081 ggcgacgacc cgtggggtgg taaacgtgca ggcttcgagg ccgaaggcaa aattaagctc
     1141 aaggacttca atatcaagac agatttaggt ccagcttctc aggaagtgga tctgattatt
     1201 tcagtggaag gcgtacagca gaagtaagag tgaaatgttg ccggatggca cgcatccggc
     1261 aatgatattt attctgttgg atcaggaatg cccagtgttg tattcagacg tccacgagac
     1321 ttattaaaga tcttattgcc attttcacgt cctgcccgac ggcgtctctg ttcttctggc
     1381 ggtaacgcgc tttcttcgca gcaaatttca ctacaacaac ctttgtattt ttccgcgcat
     1441 actggacact gaataaacag caggtggcag ccatcatttt tacagttggt atggctgtcg
     1501 cacggcgcac cgcactggtg gcaatgcgcg ataatctcat cagatatacg ttcgcccatc
     1561 cgctcgtcaa aaacaaaatt tttgccaata aaacgcaccg gcaagccctg ctcgcgcgcc
     1621 ttacgggcgt attcaataat tccgccctcg atatgccaga ctttgttgaa tccgttatgt
     1681 ttcatccagg cactggcctt ttcacaacga atgccgccgg tgcagtacat aacgattttt
     1741 ttatctttat gtgcctgcat catctcgact gcttttggca gctgctcacg gaaagtatct
     1801 gccggaattt ccagcgcgtt ttcaaagtgc cccacttcat actcatagtg gttacgcatg
     1861 tcgataaaca gtgcatcggg atcgtcaagc atggcgttca cttccgccgc ttgcagatac
     1921 tcaccaacat tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac gatacgatcg
     1981 cggaccttca tgcgcagtac ccagaaggat ttcccgtcat catccaacgc gatattcagg
     2041 cgtaagccat ctaaagccga gtcgaaggca tagagctgcg cgcgaaatgt ttcaacattg
     2101 ctcgcaggta cgctgatttg cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa
     2161 acattcagcg cggtaaacag ctgatataaa gcgtcacggg tcgccttagg atcggcgatg
     2221 tggaaatact tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa cattttggct
     2281 tttaacgcgt cgttggaaat gcggttgtgt aacactggca tggtgtacgg ttcctgcgag
     2341 atgggaaagt aaaaatccgc ggcatgatat agcaattatc gataattaac atccacacat
     2401 tttacgctac atttgcgcat taaaaattat ttgttattta caagcgcggc aatttcgccc
     2461 agtcttcagc cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat acaattgccc
     2521 gaataggttg aaaaacagga ttgatatgac gaatctaccc aagttctcca ccgcactgct
     2581 tcatccgcgt tattggttaa cctggttggg tattggcgta ctttggttag tcgtgcaatt
     2641 gccctacccg gttatctacc gcctcggttg tggattagga aaactggcgt tacgttttat
     2701 gaaacgacgc gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga
     2761 acaagaacgc cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg gactgatgga
     2821 aactggcatg gcgtggttct ggccggaccg ccgaatcgcc cgctggacgg aagtgatcgg
     2881 catggaacac attcgtgacg tgcaggcgca aaaacgcggc atcctgttag ttggcatcca
     2941 ttttctgaca ctggagctgg gtgcgcggca gtttggtatg caggaaccgg ggattggcgt
     3001 ttaccgcccg aacgataatc cactgattga ctggctacaa acctggggcc gtttgcgctc
     3061 aaataaatcg atgctcgacc gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg
     3121 cgaagtggtc tggtacgcac cggatcatga ttacggcccg cgctcaagcg ttttcgtccc
     3181 gttgtttgcc gttgagcagg ctgcgaccac gaccggaacc tggatgctgg cacggatgtc
     3241 cggcgcatgt ctggtgccct tcgttccacg ccgtaagcca gatggcaaag ggtatcaatt
     3301 gattatgctg ccgccagagt gttctccgcc gctggatgat gccgaaacta ccgccgcgtg
     3361 gatgaacaaa gtggtcgaaa aatgcatcat gatggcacca gagcagtata tgtggttaca
     3421 ccgtcgcttt aaaacacgcc cggaaggcgt tccttcacgc tattaaatct cccatgccgg
     3481 atgcttcaga atggcatccg gcattaccac agcaaatccc cctgatttag cgataaaagc
     3541 tctctggatt gcgccccctg gaagtcgggc gcataattag tgtgcttatc ttttcttctt
     3601 atgttcaccg cgcctggcgc accaacagcg gattgctatg tcaccctgtg aaaatgacac
     3661 ccctataaac tggaaacgaa acctgatcgt cgcctggcta ggctgttttc ttaccggtgc
     3721 cgccttcagt ctggtaatgc ccttcttacc cctctacgtt gagcagcttg gcgttaccgg
     3781 ccactccgcc ctgaatatgt ggtccggtat tgtcttcagc attacatttt tattttcggc
     3841 catcgcctca ccgttttggg gtggactcgc cgaccgtaaa ggccgaaaac tcatgctatt
     3901 acgctctgcc ctcggcatgg gcatcgtgat ggtgttgatg ggactggcac aaaatatctg
     3961 gcagtttttg atcctgcggg cgcttcttgg tttacttggc ggatttgtcc ccaacgctaa
     4021 tgctcttatc gccacacaag taccgcgtaa taaaagtggc tgggcgctgg gtacgctctc
     4081 cacaggcggc gttagcggcg cgttgctcgg cccaatggct ggcggcctgc tcgccgatag
     4141 ctacggctta cgtccggtat tctttattac cgccagtgtg ctcatactct gctttttcgt
     4201 caccctgttc tgcatcagag aaaaattcca gccggtcagc aaaaaagaga tgctgcacat
     4261 gcgggaagtg gtgacatcac ttaaaaaccc gaaactggta ctcagcctgt ttgtcaccac
     4321 gttaatcatc caggtggcga cgggctcaat tgcccccatt ctgacgctgt atgtccgcga
     4381 actggcgggt aacgtcagta acgtcgcctt tatcagtggc atgatcgcct cggtgccagg
     4441 cgtggcggct ctgctgagtg caccacgact cggcaaactt ggcgatcgaa tcggccccga
     4501 aaagatcctg attacggcgc tgatcttttc tgtactgctg ttgatcccaa tgtcatacgt
     4561 tcagacgcca ttacaacttg ggattttacg ttttttgctc ggtgccgccg atggtgcact
     4621 actccccgcc gtacagaccc tgttggttta caactcgagc aaccaaatcg ccggacgtat
     4681 cttcagctat aaccaatcgt ttcgtgatat tggcaacgtt accggaccat tgatgggagc
     4741 agcgatttca gcgaactacg gtttcagagc ggtatttctc gtcaccgctg gcgtagtgtt
     4801 attcaacgca gtctattcat ggaacagtct acgtcgtcgt cgaatacccc aggtatcgaa
     4861 ctgatttttc gcctttcata cttgcaaaag cggagaatca gctatccttt tccctgaaac
     4921 ctcatcaact caaagggaga atcgtgatga ccatgtacgc aacgcttgaa gaagccattg
     4981 acgctgcacg cgaagaattt cttgcagaca accccggcat cgacgccgaa gatgcgaatg
     5041 tgcaacagtt caatgcccaa aaatacgttt tgcaggacgg cgacatcatg tggcaagttg
     5101 agttttttgc cgacgaaggg gaagaaggtg aatgtttacc tatgcttagc ggtgaagccg
     5161 cgcaaagtgt ttttgatggc gactatgatg agatagagat ccgccaggag tggcaagaag
     5221 agaatacatt acatgaatgg gacgaggggg agtttcagct tgagccaccg ctggataccg
     5281 aggaaggacg cgcagcagct gatgagtggg atgaacgtta atcactcata cgggccatga
     5341 tgaatgtcga tcggcagcag taaagtatca aaaaccaggg agaatggcag atcaaggatt
     5401 gtgacataac gccaggcgga gtcacgcaca tcccattgca caccgggata atattggttg
     5461 ccatgcccct gccccggtat agtgcggcta atgatgctgc cacagccact cagcaaggtc
     5521 accatgatgc tcaccacaat taaacgcatt tgatacctct tccgtaaaaa aataccggca
     5581 taacgccggt atttttattg gctcgttgtt tttgccagat gcgacgtgaa cgccttacca
     5641 aacccacaaa atcttgcaaa atcaataaat tgcaggaacg atgtaggcct gataagcgta
     5701 gcgcattagg caactacgtt tttattgcga agccgcatcc ggtttacgca atgccagtgg
     5761 attgagcttt atcccttcgt aataactcac ccatgaagaa tatctctcag gggaattcca
     5821 gacgcggaaa tgcagacggg ccatcgtcac cggatcgctt agcagcacaa ggcgacgatc
     5881 gcgattcagc ttctctggcg tctcgttcag cgcctgttca acgtggcggt cacgggcgat
     5941 ttccagcacc ttactggcgc ggtgacgcgc ggtcgccatt gcggttgcca gagcgttaaa
     6001 tgacgggtta aacactgcgt gcataaaacc atcatcaagg gagcgttgac ggttcatctc
     6061 aaggaaccga tcggtatcaa ccagtacctg cggcggtgaa tactcttccg ggatcaggaa
     6121 cagtttccag cgtttggtgc gcagaccaac ggtggcacgg ctggaaatca ccgaaacaaa
     6181 cggtgacagg atcaacgaga agacaatcgg tgccagccag aacaggaaac gcagatccaa
     6241 ccacgccatc ccaacagccc acactaaccc taacagcagc tgtgagccgt ggcgtttgaa
     6301 cgcttccccc caggaagtgg agtcatcatc acgctgtggt gaattccaca ccacttccca
     6361 gccaaggaac gcgctgacga cgaagaccgt atggaacagc atgcgtaccg gagccagcag
     6421 cacggaaaac agcacttcca gcagcaacga taatgtaacg cgccagaagc cgccgtattc
     6481 tttcgttcct ttgcaccaga taagcaaaat gctcaataac ttcggcagga acaacagcac
     6541 catagtcgaa gcaaaaagtg caatcgccag ctcaggacgc cactgcggcc acaccgggaa
     6601 caactgccgt ggttgcagga agtattgcgg ttcggtcaac gcatgtacta cctgcaatgc
     6661 agtagagagc gcgaggaaca taaaccacag cggagcggag agataagaca tcacgcccgt
     6721 caggaacacc gcacggtgaa ccgggtgcat acccttaacc aggaacagac ggaagttcat
     6781 caggttaccg tggcaccagc ggcggtcacg ttttagctca tcaagcaagt taggcggcaa
     6841 ttcttcataa gaacccggga gatcgtaagc aatccagacc ccccaacctg cacggcgcat
     6901 caacgccgct tctacgaagt catgtgacag gattgaaccg gcaaaggaac cttcgcccgg
     6961 cagcggagcc agtgcgcagt gctcgataaa cggtttcacg cggataatcg cgttatgtcc
     7021 ccagtagtgc gactcgccaa gttgccagaa gtgcaaaccg gcagtgaaca gtggcccata
     7081 cacgcgggtc gcgaactgct gacagcgcgc atacagcgta tccataccgg acgctttcgg
     7141 cgacgactga atgatcccgg cgttcgggtt ggcttccatc aagcgcacca ggccgcataa
     7201 acaatcaccg gtcattaccg agtcagcatc cagcaccacc atgtagctgt actggctgcc
     7261 ccagcgacgg cagaagtcat cgatattacc gcttttacgc ttcacgcgac gacgacgacg
     7321 gcgatagaaa atctgacctt cgccaccgac ttcagcgata agctccatcc aggctttttg
     7381 ctctgcgacg cagatatccg ggttgtaact gtcactcaga atgtagacat caaagtgttt
     7441 ggcattcccg gtggctttta ctgattccca cgttgcacgc aagccagcaa aaacacggtt
     7501 cacgtcttcg ttacagatag gcatgatcaa cgccgtgcga tgctccgggt ttaatggttc
     7561 atcgccaact gttgacgcag atatactgta tttatcgcga ccaataagca gttgcaggaa
     7621 gcccattaac gccgtccaga atccggcgga cacccaacag aacagtaccg caaagaggat
     7681 caggataccg gtttgcagca tataaggcag aagctgcata aaggaaaccc acaaatcctg
     7741 accaaccata tccataggat taatcagcgc ccaaccctga taaggaagaa tggtcttcat
     7801 ataccaggtc gcgacgacag tttgcgcgag cgtcaggatc aacagaatgt aacggcggat
     7861 ggtaccgacg gtacgccact tttgctcgct ctcctgctct tctttggtca aacgagccag
     7921 atagcgcggc gttacatcgc gtccacgcag gcgatcccag aaacggccta ccgggttggt
     7981 acgccacggg tcgggaaaca tcgaggagcg ttttgcttct ggcatcgcct taagctgatc
     8041 gcgcccttcg tcatctttaa ttaactgtcc atcagcaagt gaatctggcc aggcttgttc
     8101 cagacgcgcc tttaccgagc cttgcgggga gtcatcctcc cgcgcccagg tgcggtgttc
     8161 ggcatccagc gcctgatgaa cggcgcggat atcagtcttc ggcaatgccg ctttctcgct
     8221 tgcggcgatg ggcattgcgt caatgtactc agttgtctta ttcattggca ggtaactggt
     8281 agctccaggt ttcactcaac gtctgatcgg cattcaccag cgcagcacgc atttcagtgg
     8341 ttttcttggc atctttcact ttcacacgca tcaccagacg ccagcctttg gtaaccgggt
     8401 tatagcgcac cgtgctttcc actatctcac cattatcacc aatgctggtt tgcgctgtga
     8461 ccggggtatc ctctggcagt tttttcatct ctgcgccggt aaaatcgacc acaaaggcga
     8521 tagtaccgtc aggctggcga atcaggttcg actgcttcac atcccccgtt gaacgacgcg
     8581 tttgttgcac ccatgcgtta tctggcgcat gcagtttgtc ttcatcacgg ctgaaggtga
     8641 tggtgtattt aaagttcatc tctttacccg gctccggcag ctgatccggc gtccagtaag
     8701 cgacgatgtt atcgttagtt tcatcgttgg ttggaatttc caccagctca acgctgcctt
     8761 tgccccactc ccctttcgga gtcacccatg cgcttggacg gagatcgtaa cgatcatcga
     8821 gatcttcaaa gcgggagaaa tcacgaccgc gctgcaacag accaaagcct tgcgggtttt
     8881 ccatggagaa gctgctgacc gctaaatgtt tcgggttatt caacggacgc cagatccatt
     8941 cgccattacc ggcatggata gagagaccgt tagagtcgtg caactccgga cgatagttat
     9001 ttgcaggcga cggttggttc ggcccaaaca ggaacatact ggttaacggt gcaaccccca
     9061 gtttgccgac tttatcgcgc agatagattt tcgactgcac atccacaacc gtgtcacgcc
     9121 ctggcataac cacgaatttg taagcacctg tcgcgcgcgg cgagtcaagc aatgcataaa
     9181 tggttaaacg tttatcagtc ggttttggac gctcgatcca gaactctttg aagcgtggaa
     9241 attcttcacc cgatggcaag gcggtatcaa ttgccagacc gcgggcagaa aggccataaa
     9301 cctgacctgc accaatcacg cggaaatagc tggccccgag catgctgacg atttcatcgt
     9361 ttttatcttt gctgttgatc gggtaaagca ctttaaaacc ggcaaaacca aggtctttta
     9421 ccgtgtcttt gtcatgctga acatcgccga aagtgaaata atccgggctg tatttgattc
     9481 gtttgactgc ggtggcagtc acttcattta ttttgaccgg ggtatcgaag tacataccct
     9541 gatggtagaa ctcgagtttg aatggggtct tcagattgtt ccagtacgct ttgtcatgat
     9601 taaactggat ctgctgatag tccgcgtatt tcatgtcgcg gaaaacggag ggcaagttgc
     9661 ttttgggcgc ctcatagcct ttcccggcta aggattgagc ttgctttgcg acatcatcaa
     9721 tactgaaagc ccagcttgaa gatgtataca gggttaacat tactgcagca ctcaaccaac
     9781 gcattttcat catttgtagt ttatgtttca taattagtaa gcacttcccc ctttgtgtgc
     9841 ttatatcgat ccgatctatt ttaatggata accagccatt ccgacaacag aatcccggta
     9901 tcgttcagca cgctggctca tggattaagc acctgattgc aaccgttttc actttccgtg
     9961 cttctcctaa agacagaagc atcgacttcg tgtagggttt gccagcaaat ttatttatca
    10021 accgtcttac agacgcttcc attctggtta gtggtagcga tgatgctatg cataaaaacc
    10081 ttgaagcctg cgcctggcgc ggtcaggtcg gtgtcgctat ggcaacaagc ctaaaggcat
    10141 aacccaacag ggataagacg aaaaatccga gattagttaa ccatatatga atccagtacc
    10201 cgcgcaacgt gaatatttcc tcgactccat ccgcgcctgg ctgatgttgt tagggatccc
    10261 ttttcatatt tctttaatct attcgagcca tacatggcat gtgaatagcg ccgaaccgtc
    10321 attgtggctg acccttttta atgacttcat ccactcgttc cgcatgcagg tatttttcgt
    10381 tatatccggg tacttttcct acatgctttt tttacgctat cccttgaaaa aatggtggaa
    10441 agtacgtgtc gaacgtgtag gtatcccgat gttaacagcc atccccctac tgacattgcc
    10501 gcaatttatt atgctgcaat acgtcaaagg gaaagcggaa agttggcctg ggctgtcatt
    10561 gtatgacaaa tataatacgt tagcctggga attaatatca cacctgtggt ttttactggt
    10621 gttagtggtc atgacgacgc tgtgcgtatg gatatttaaa cgcatcagaa ataatttaga
    10681 aaattctgat aaaacgaata aaaaattctc gatggtaaaa ctatcggtga tttttttgtg
    10741 cctcggcatc ggttatgcgg taataagaag aacgattttt attgtgtatc cgcccattct
    10801 gagtaatggc atgttcaatt ttattgtcat gcaaacgcta ttttatttac cgttctttat
    10861 cctcggcgca ctggctttca ttttccctca tcttaaagcc ttgtttacca cgccttctcg
    10921 tggctgtacc ctcgctgcag cattggcgtt tgtcgcttat ttactcaacc agcgctatgg
    10981 cagtggcgat gcctggatgt acgaaaccga gtcggtgatc accatggtcc tcggtctgtg
    11041 gatggtgaat gtggtcttct cctttggcca ccgtttgctt aacttccagt cagcgcgggt
    11101 gacttacttt gttaacgcat cgctatttat ctatctggtt caccacccgt taacgctgtt
    11161 tttcggcgcg tacattacac cgcacctcac ctccaactgg cttggttttc tctgtggcct
    11221 gatattcgta gtagggattg cgataattct gtatgaaatt catttacgca tcccattact
    11281 gaagtttttg ttctctggta aaccggttgt taagcgtgag aacgataaag caccagcccg
    11341 ttaagccgcg tttacaataa ccattccacg ggcaatatcg acgccagtct gaccataacc
    11401 cgcttccaga aactggtggc gggttctttt ttgagaacaa tctctttctt tgcatgacga
    11461 tcaacccagt tgatccgccc ccacctgtcc agacggagct gccaggccgc atcatactgg
    11521 ctctgaataa agcgtttatc aattaactgt gccagcgttt cgctctctat cacgaagccc
    11581 atttcagtat tgagcaatgt tgaacgcgga tcgaaattga aagagccgat aaacaccgtt
    11641 ttaccatcga tgctaaaggt tttagcatgc aggctggcac cggaattacc ggttatgccg
    11701 cgatcgtgta acgtactact ttgttcacgc gtcggcttga gttcatataa ttccacgcca
    11761 tagcggagca attttttgcg ccagcgcgcg tatccggcat ggacgacagc aacatcgtta
    11821 gcggcaagag aattggttag gatcgcaatc tttacccctt ttctcaccat ccgtaagagt
    11881 tgcgccacac ctgcgcgtgt cggtacaaaa taggaagaga taatatcgat gcgttcactg
    11941 ggtgagccca tgatatcgaa caggcgctgc ggtaacagtg aatgccgttt tgccttgccc
    12001 tcccctttcg ccggatcatc acttaataaa cgtgttttcg cccagataag cggcaacgtt
    12061 ccatcaacca gatgatttat aaacggactg gattccattt tgcgtaagta acgatgcgtc
    12121 atagcatcat tatgccagga ggcgggtaac tcgatgcgat ccgccatttc accttccggg
    12181 acatccagca cctgctgtaa gggtgaaacc gatttgcaat accagtagcg ggcgaaatca
    12241 tcggcaacgt cctctactac gggtcctatt gccatgacat ctaaatccga aaaaagtggc
    12301 tcttcccctg ccccaaaata ggcatcacca atatttcgcc cccctaccag cgtcaccacg
    12361 ccatcgacag tgaaactttt attgtgcata cggcgattga gacgggaaaa gtcggtgata
    12421 taaccaagcg gacgcagcaa gcgaaacgag aaaggattaa aaagccggac ttcaatgcgg
    12481 ggatggctgt caagcaagcg taaaatgtcg tcaagtccgg gcgtattgtt gtcgtccagc
    12541 aacaaacgga cgcgaacgcc acgctttgct gcggctaaca gggcggaaaa cagtaaccga
    12601 cccgacatat cgtcctgcca gatgtaatac tgaacatcca gcgtatgctc tgccatttcg
    12661 gccagacgat accgggcggc aaacgcatcc agacttttct ccagagggaa aagaccacac
    12721 tgaccgggat gttgcgaaca cagtggcagc accgcgctcg ccagccgggg caaatcattc
    12781 atctccttgt tgggtaagga gtctttcgta gaggtgggcg ttttcttcat cataacagac
    12841 aaagtatacc tgttcgggta aagcgtgacg ggtaataaat tctgaaacgg tttttactgc
    12901 gatttcagcc gctgccgcac gagggtaacc ataaacccca gtactgattg caggaaaagc
    12961 cactgacgta tagctgtttg ccgccaccag tcgtaggcta ttgagatagg catcctgcaa
    13021 aagctggtct tcgttttgtt caccaccacg ccagactggc ccgacggtgt gcactacggc
    13081 tttagcggga agatcgcctg caagcgtaat aacggcatgc cccgtagggc aatcgccctg
    13141 ctgttgcctg acttttaaac aggcatccag cagggccgga ccagctgcgc gatgaatggc
    13201 cccatcgacg ccgccacctc ccattaatga aggattagcc gcattcacaa tcacatcaac
    13261 ggccagtttg gtaatatcac cctgcacaac atgaatacgc gttttcataa cgcctcctta
    13321 caccttcttg tttcttaagt gtacagcagg ctttttggtt aaatcgcatt tttctgctca
    13381 gcaacgtgag ttgttctgaa acttagttat attcgatgtt cattacagcc agctttttct
    13441 gtttatcaag atactgcact tttactgagg ctatttgcgc gtttttgaca ttgccgctca
    13501 ttaacgcttt gctggagtag gttttacccg gaacagaacc attctgtggg cagttcattt
    13561 ctacgggtga tgactgggtg ctgacgtcac atgccggttc cacaatttgg ccataaaaat
    13621 ggatcacgcc cccttgcgcg gtgcttcctg cgatggcaat aaaaggaaaa aacaaactgc
    13681 cgagcataag agagtttaaa aaaggtctga acatttattc tatttcctca atgattagtc
    13741 atccttgagg gttgtgttat ccatactttc aggttggctg attcgcagca gaccattctc
    13801 tccagattca tcttatgctc gatatttcaa caaattaaga cttttctgaa gagggcggcc
    13861 attgttgtga taaatgaagt gactgtccat cagaaacagt aacaactatt ttcacccgat
    13921 cgtccgggga aatatttaaa ctcaacttcg tcaaagcaat gggttgatta gcaggcaatg
    13981 aaagggtctt ttcttgcttc gtctgacttt gccctgaact gccttcgcgc agggacaata
    14041 tttgtactct gcacagacaa gattgagtaa gagtgacttc aggaataatg gtatacacat
    14101 ccccttgctg ggtcgtatta aaggttatct gactggaaag tgccgcaagg agtaataacg
    14161 cattcatatt cttctcccga aaaaaaacag ggcttgcgcc ctgtttctgt aatacaaatg
    14221 atgtattagt actgatgagc ggtcgcgttg ttaccaaagc caacctgagt cacgttgacg
    14281 gaggagttag atgcagtctg gtcaactgca gcaccgttgc caccaccgaa ctgtttaacc
    14341 gtcatttcag aatttttgcc gttccactga tcaagagtag cgctgttacc gaagccacgt
    14401 tgggtcagat cgattgagct gtcatccgag ccctgaccaa catctgcacc attaccgccg
    14461 ccatgctggg taatagtcaa gtcagagtta cgggcatcag tttgcagagc aagtgcagag
    14521 ttaccgccac cgtactggta aatgttcagc tcagaatttg ggccgctatt attaccgcca
    14581 ccaccgtggt taccgccgcc gccgtactga ggaacaacac ctgccagagc gctaccggag
    14641 aatacgattg ctgcaattgc tgctactttt aaaagtttca tgtaaaaccc ccatcggatt
    14701 gatttaaaag tcgaatggaa attaacgttg tgtcacgcga atagccattt gcgactgtct
    14761 ctgcactaca attgccgttt tttgagtacc atactgtgta atatttgctt tattaccaga
    14821 acctttctgg ataatcatcg cagtattacc ataagcacct tgcgaaatac tggcatcgtt
    14881 ggcactgccc gcctgatcaa tatatgcaag gttataatct cctgtctggt caatctttgc
    14941 ccggttgcta ctaccttctt gcgcaacaac cgccaaaagt tttgagcctc cctgccgtaa
    15001 ctgagcacta ttattagtcc cagcttgacc aattatggct gcctgattaa atgaagactt
    15061 actcaattca tttaccgcga agttatattc tgaattagct aaatcataac ctgctgcggc
    15121 tgcaatccca ggcgcaccca gtattgttaa catcataaat aacaatttgt ttttcatgtt
    15181 gtcaccctgg acctggtcgt acatttaaga aattaaatca tttcaacttg gttgttaacg
    15241 caacctgtat tttgttaatg ctgcgttacg atggaaagta tgtctgcgga aatattttta
    15301 ataactcacc cgcgcgttgt atttttcttt ttttgttcat accaaagtat taaaagccac
    15361 gtaaatacgc tgttatctac gcaaaaaata ttttgttttc ttttaaatct ccgtttccgc
    15421 tatcaaaaat caccagacag tcattcttct tgcccgtcgc tgattgctgc gatatgtctt
    15481 gcgcacaagc cgtgacaaac ctcgtctttt tgcagtaaaa aattgtccac ggaggtgtgg
    15541 agaaaaaaca acaacttttt acatgacgaa aggactacac cgaaatattt tttatatgca
    15601 ttattagtaa gttatcacca tttgtatgat tttttaaaat tgtgcaataa aaaccaaatg
    15661 tacaactttt c
//
LOCUS       DAZFFG010000104        15519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFFG010000104 DAZFFG010000000
VERSION     DAZFFG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.104"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(37..318)
                     /gene="yehE"
                     /locus_tag="ACHJ7J_003769"
     CDS             complement(37..318)
                     /gene="yehE"
                     /locus_tag="ACHJ7J_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO5001949.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(580..1689)
                     /gene="apbC"
                     /locus_tag="ACHJ7J_003770"
     CDS             complement(580..1689)
                     /gene="apbC"
                     /locus_tag="ACHJ7J_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO5001950.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1821..3854
                     /gene="metG"
                     /locus_tag="ACHJ7J_003771"
     CDS             1821..3854
                     /gene="metG"
                     /locus_tag="ACHJ7J_003771"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO5001951.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     VLIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3995..4189
                     /locus_tag="ACHJ7J_003772"
                     /pseudo
     CDS             3995..4189
                     /locus_tag="ACHJ7J_003772"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            6722..7798
                     /locus_tag="ACHJ7J_003773"
                     /pseudo
     CDS             6722..7798
                     /locus_tag="ACHJ7J_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7811..10843
                     /locus_tag="ACHJ7J_003774"
     CDS             7811..10843
                     /locus_tag="ACHJ7J_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO5001952.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHITWGKVGSNGQSQVKSFAD
                     ATAAEKAELKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTFLEICFCQGWNDETSSELLDEIVS
                     TFGIEYATETVIFWWQIDFDFDYKDEHLTFFINYADSSKDSYRRNNDKFSLRLRKHLS
                     LAEEEVWQNCIAKLLVALPDISLYRQPLIAILMPEIPEVAHEIVHRLHQVADVPQLEM
                     LKLVATDPFTLEILENYQYIDVFNTYGASWSATVLREQGIAALARLASYAEEDNCGDV
                     LKCINHPLAITALIEAADTNKRCYERLIKSAENFPSATIAALAEALVKKDDKRKHTLL
                     VSLLNANPLLVEKLVIGLSSQAANLLQQCQQLLNSRNEDFANSEILPMVLVSPPWETQ
                     NKNNDNSPVGKYPAKIPPLPLFYQPPLWTRPKLKNSSKVLPDNALLHLGEMLRFPQEE
                     ALYPGLLQVKDACTADSLAEFAWDLFTAWLTTGAPSKESWAFTALGVLGNDDTARKLT
                     PLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERAKEKIADIA
                     ESRELTVEELEDRLAPDLSLDDNGSLLLDFGPRQFTVSFDETLKPFVRDASGSRLKDL
                     PKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLESAMCLRRRWSPENFQLFLVE
                     HPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYSTADDDLFTLPEGDISIGIPHVLE
                     ISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAGRKCPSGRVMGL
                     ANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSAEQLLSKLWLWE
                     GKAESYGWGSNSTQEAQLSVLDTITASELINDIEALFE"
     gene            10853..14485
                     /gene="yehI"
                     /locus_tag="ACHJ7J_003775"
     CDS             10853..14485
                     /gene="yehI"
                     /locus_tag="ACHJ7J_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO5001953.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLVAIPRLAMYAHKEDCGSLLVQINHPQVIHTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPTRPGYPIIEDKKLPAQQKARDEYWRTLLQTLTASQPQLAEE
                     VMQWLSTQARAVLNSYLLAPPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELVFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHVEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFLHRPKETFPLILNFGATELALPVARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     VFIKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRADVWPALEQLLKQGPMDIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALMQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPILGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     GYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSETLSA
                     PDVIFH"
     gene            14546..14863
                     /gene="yehK"
                     /locus_tag="ACHJ7J_003776"
     CDS             14546..14863
                     /gene="yehK"
                     /locus_tag="ACHJ7J_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO5001954.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRRER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGKSPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         4173 a         3874 c         3725 g         3747 t
ORIGIN      
        1 cggctttcta aatgcaatta aaatcaggtt gccaaattaa tcgtagcgat ttaacacgat
       61 tcgacgtttg ctatcactac ctgcaacaac cacttctgtc gttacacctt tgacgggcga
      121 agaaacctga tgacgggtat cgacattata cgttaactga ccacaatgct gttgaggttg
      181 actattaacc atagccatac tgcatgttgg gggactgatc ctgccattaa tcgttaatgt
      241 ggcagtctct gaagagaacg cgggagaagc caatccatag gcaagaaaaa taattcctga
      301 cagccaatac ttattcattg aacgttatcc ctgtagtaaa ggttatgcct ggatagaatg
      361 agtgcataac aaactatagc tgtacatcca ctacacagca cgaaggatga taatgaagca
      421 ttgcctgtac gatcaatcga ctttgtagag tttcggacga aggtccgcag aatattcgca
      481 gtattaaata agtgttcatt aatgcggcaa aaacaaatac accgcatcaa tgtaacatct
      541 ctgcggtgta tgaagaataa ccagaatggt taataggcgt tagaccgcgc ggaaggaaat
      601 ctcgcctgga atgacttcac cctgccagta gagctgagct gcaacgcggt cagccagctg
      661 gcggtagata gcggtaaatt cgctctccgg acggctgata accgttggcg tgcctttatc
      721 gagatcctca cgtaaggaaa tatgtaacgg catctgcccc agcagttgtg tgtggtattt
      781 ctctgccagt ttctctgcac caccggtacc gaaaatcggt tcatggtgac cacagttact
      841 gcaaatatgc acactcatgt tctcgacaat accgagcacc ggaacctcga ctttctcgaa
      901 catcacaatg ccttttttcg catcgatcaa cgcgatgtcc tgcggggtgg tgaccacgac
      961 tgcaccagtt actggaatgt tttgcgccag cgtcaattga atgtcaccgg tgcctggcgg
     1021 catatcaagc accagatagt cgagatccgg ccacaaggtt tcctgcaaca tctgcattaa
     1081 cgccttgctc gccatcggtc cacgccacac catcgcattg tcgtcagtga ccagataacc
     1141 aattgaattg gttgccaggc catgagacat aatcggtgcc atatgggtac cgtccggcga
     1201 agttggacgc tgattttctg cgcccagcat ggtggggatt gatgggccgt agatatcggc
     1261 gtccagaatg ccgactttcg ccccttccgc cgcgagtgcc aacgccaggt ttaccgccgt
     1321 ggaggattta cccacgccgc ctttaccgga actgaccgca atgatgttct tcacgccatt
     1381 aatacccggc tgattcttca cgcgttttaa ggtggcgata ttgtgcgaca gtttccagtc
     1441 gatagctttc gccccggtga tacgcagcag ttcggcgcta cattgctctt ttaactcttc
     1501 aaacgcgcta tgccagacga atggcataac cagttccaca tgcaacgtgt cgtccatcca
     1561 ggcgacatgg tgcaacgctt tcagcgtggt gaggttatgc ttcagggttg ggtgctgaaa
     1621 attggccagt gtcccggcga ccattgcgcg caaggcttct ggtgatttgg cctgggattg
     1681 ttcgttcatc ccgactcctt ttttgtatag ataaaccatc agctgatagt ttacctgaag
     1741 aatatagaga agtacttact taacattttc ccatttggta ctatctaacc ccttttcact
     1801 attaagaagt aatgcctact atgactcaag tcgcgaagaa aattctggtg acgtgcgcac
     1861 tgccgtacgc taacggctca atccacctcg gccatatgct ggagcacatc caggctgatg
     1921 tctgggtccg ttaccagcga atgcgcggcc acgaggtcaa cttcatctgc gccgacgatg
     1981 cccacggtac accgatcatg ctgaaagctc agcagcttgg tatcaccccg gagcagatga
     2041 ttggcgaaat gagtcaggag catcagactg atttcgcagg ctttaacatc agctatgaca
     2101 actatcactc gacgcacagc gaagagaacc gccagttgtc tgagcttatc tatactcgct
     2161 tgaaagaaaa cggttttatt aaaaaccgca ccatctctca gctgtacgat ccggaaaaag
     2221 gcatgttctt gccggaccgt tttgtgaaag gcacctgccc gaaatgtaaa tccccggatc
     2281 aatacggcga taactgcgaa gtctgcggcg cgacctacag cccgactgaa ctgatcgagc
     2341 cgaaatcggt ggtttctggc gctaccccgg taatgcgtga ttctgaacac ttcttctttg
     2401 atctgccctc tttcagcgaa atgttgcagg catggacccg cagcggtgcg ttgcaggagc
     2461 aggtggcaaa caaaatgcag gagtggtttg aatctggcct gcaacagtgg gatatctccc
     2521 gcgacgcacc gtacttcggt tttgaaattc cgaacgcgcc gggcaaatat ttctacgtct
     2581 ggctggacgc accgattggc tacatgggtt ctttcaagaa tctgtgcgat aagcgcggcg
     2641 acagcgtaag cttcgatgaa tactggaaga aagactccac cgccgagctg taccacttca
     2701 tcggtaaaga tattgtttac ttccacagcc tgttctggcc tgcaatgctg gaaggcagca
     2761 acttccgcaa gccgaccaac ttgtttgttc acggctatgt gacggtgaac ggcgcgaaga
     2821 tgtccaagtc tcgcggtacc tttattaaag ccagcacctg gctgaatcat tttgacgccg
     2881 acagcctgcg ttactactac actgcgaaac tctcttcgcg cattgatgat atcgatctca
     2941 acctggaaga tttcgttcag cgcgtgaatg ccgatatcgt taacaaagtg gttaacctgg
     3001 cctctcgtaa tgcgggcttt atcaacaagc gttttgacgg tgtgctggca agcgaactgg
     3061 ctgacccgca gttgtacaaa accttcactg atgccgctga agtgattggt gaagcgtggg
     3121 aaagccgcga atttggtaaa gcagtgcgcg aaatcatggc gctggctgat ctggctaacc
     3181 gctatgtcga tgaacaggct ccgtgggtgg tggcaaaaca ggaaggccgc gatgctgacc
     3241 tgcaggcgat ttgctcaatg ggcatcaacc tgttccgcgt gctgatgact tacctgaagc
     3301 cggtactgcc gaaactgacc gagcgcgcag aagcatttct caatacagaa ctgacctggg
     3361 atggtatcca gcaaccgctg ctgggccaca aagtgaatcc gttcaaggcg ctgtataacc
     3421 gcatcgatat gaagcaggtt gaagcactgg tggaagcgtc taaagaagaa gtgaaagcag
     3481 ctgccgcgcc agttactggc ccactggcag atgatccgat tcaggaaacc atcacctttg
     3541 acgacttcgc caaagttgac ctgcgcgtgg cgctgattga aaacgcagag tttgttgaag
     3601 gttctgacaa actgctgcgc ctgacgctgg atctcggcgg tgaaaaacgc aatgtcttct
     3661 ctggcattcg ttctgcttac ccggatccgc aggtactgat tggtcgtcac accattatgg
     3721 tggctaacct ggcaccacgt aaaatgcgct tcggtatctc cgaaggcatg gtgatggctg
     3781 ccggtcctgg cgggaaagat attttcctgc taagcccgga tgctggtgct aaaccgggtc
     3841 atcaggtgaa ataatccccc ttcaaggcgc tgcatcgaca gcgccttttc tttataaatt
     3901 cctaaagttg ttttcttacg attttgtctc tctctaaccc gcataaatac tggtagcatc
     3961 tgcattcaac tggataaaat tacagggatg cagaatgaga cactttatct atcaggacga
     4021 aaaatcacat aaattctggg cggtggagca gcagggaaac gagttgcata tcagctgggg
     4081 aaaagttggt acccaagggc aaagccaggt aaaaaatttt gctgatgttg cagcagcgga
     4141 aaaagcgaaa ctgaagctta tcgcggagaa aacgaaaaaa ggctatgtgg aaaatgcttc
     4201 agcaaacgtg catatccccc ccattaccaa agccactcct gaagttgaaa cttcccctga
     4261 gagtaaaaac caacgcccct ggctggctga tgatgcggtc attcggctaa ctgacgatat
     4321 taatcgattt gcttttcctc atcgctctcg ccccagagag atcaattatc tgcacaaaga
     4381 tggtcaaata tggcagctca tcgcgaataa caccagggca tatgatcccg ccaacaatta
     4441 ccgttcttat ccagaaaact ggcagcaggc ttttgctgag ttacaaatgc gagttcaggg
     4501 taatcaacag acagggagtg cacaatctga tgcagcattg ctctggtgtt tctggaactc
     4561 ttactctgca gatgaactgg tcgatgactt agtcattcgc tgtggtctgg aaagtgccgt
     4621 tgaaattgcc cttctcgctc ttcaacttag atataaacca gtaaaaggcg cagtaaccac
     4681 catcattcca cctaatcacg aagcagaatc gctacctagt tggcatcaac gtctatgtca
     4741 tcatctttca ctcgcttcag aagatgagtg gcaacgctgt gtagataaag tactcaccgc
     4801 cattccgacg ctatcccccg cacggcaacc ttttgcggcg ttactgctcc ctgaacgccc
     4861 ggatatcgcc catgctatag ctctacgcta tgcagaccaa aacgttccgg cgatgacctg
     4921 gttaagtatg atggtaagcg atgatgttgc cctggccatc caggaaaaat atggtttccc
     4981 accgctatat aatgatttcc gcaaatatct ggctacgttg ctggcaaata atggaatgcg
     5041 tggtgtaagc cggattcttc tcaagctacc tgtcgactat cccgtaaaat acacggacct
     5101 tttcactcac atacacgcca atgctgaaga tcttgtcaaa tggctatgga aaacgaatca
     5161 cccggatgcg attcaaattc tgatcctcgg tgtaaatggc aagaaaaagc acctggaata
     5221 cttaagcaaa gcctgccaaa aacatcccgc tgcggctatt gccgcttatg ctactttgct
     5281 ggcaatacat gaaaataatg agtggcgtaa agcgcttgtc aaactgatta ccgccacgcc
     5341 agagttagta tgcgaggtca ttccctgggt taatgctaag gcggcaggca tcttatctga
     5401 atgtcgtccg ctgccagtag ctgaagaatg tgaatatgcc actgtggata tgttgccgga
     5461 gctgcttgtt tcgccgccct ggatgaccaa agagaaaaag aaaaatacgc ctgtgtttga
     5521 tcttccagtg cttccagttc cttctgtttc tgatgtcact ccagagatta ccaaaaaact
     5581 gacccgtact taccttgtca cccacttcca gcaaatagcc caacagcagg cgacaaaaca
     5641 aacattgttt accgacctgc cgccgatcaa aaaagcaagc tgggagaaac acttaatacc
     5701 gcttacaccg gagcaacaaa tactgtggca tcttggtttt gaaaaatggc gggaaagcgg
     5761 agaaaaaata tacgaaaaaa ttccggcgcc acaaagcgcc gttgatgccc tgctacgctt
     5821 cgattttcca gcactcaatg ctgaatttgt gcaatatcat aacaatgcat acaaaagctg
     5881 gaatctcatt gccctctgtt acctgcctgg tcaacaggcc atttcttttc ttaaccagat
     5941 agtcaaagaa gataactact ctggtgaggg aaatattctg gcaatttttg gtagcgccgc
     6001 catacctgca tttatggcct gcctgcaacg tgatccacga cgtctgtgct tcttcccctt
     6061 tttcctcggt gtcagtgaac tggccttacc gatggcgcaa caattacaga aaaaaatgtc
     6121 ttatgaagat gcgcgaaact ggctaactga ttatccacgc catgccgccg ccgggctact
     6181 tcctgtggcc ctgggcaaaa aaggaaaaga tcgtgattgt gcccgccagg cgcttcgtct
     6241 gctggtgaat ttaaaccagc gagaaacgat tgaggaaatc gcgcaaggat ataatcaacc
     6301 tgatgtttta gctgcgctgg caacattgtt cgatagcgac ccactggaag aatatcccgc
     6361 taaaatcgcc ccactgtccg gcttttatca gttcaccttg tggcgcagac cacggcttaa
     6421 aagtaacaac ctgcctctgt cagatgacgc tatgcgccac ctcggcacta tgctgagctt
     6481 tcctcgcgac attaccgcct acgctgggct ggatatcatt agagaaatct tcacccgcga
     6541 atcactggct gaatttggct gggatctgta taccgcctgg acagaagctg gcgcacccgc
     6601 aaaagaaaac tgggcattta cttcgcttgg gattttaggc aatgacgaca ccgcgcgtaa
     6661 attaaccccg cttatccgcg cctggcctgg ggaatcccag cataaacggg cagtgtccgg
     6721 gctggatgta ttagctgata ttggcagcga tgttgcgcta atgctgctta atggcatcgc
     6781 gcgaaaaatt aaattcaaag cattacagga acatgcccgc gagaaaatca acatagttgc
     6841 tgaaaaccgt gggctgacta tggctgaact ggaagaccgc ctggctccag atttagggct
     6901 tgatagcagt ggctcgctga tactggattt cggcccccgc aaattcaccg ttggttttga
     6961 cgaaacctta aaacctgtag tgtgcgatgc aaacggcaaa gtcctgaaag atttacctaa
     7021 gccaaaccag agcgatgata aaacactggc aactgacgca gttaatctct tcaaacagtt
     7081 gaaaaaagat gttcgtgcta tcgccagcca gcaaattacc cgtctggaac aggctatgtg
     7141 ccagcgcaga cgctggacgg cagagcagtt ccgcctgttt ctggtggagc atccgctggt
     7201 acgtcactta acccgacggc tgttatgggg ggtttataac gatgaaaacg ctcttatcac
     7261 ctgttttcgc gtggcagaag acagcactta cagcgatgcg caggatgagt tattcacgct
     7321 gccagcagga aacatcggta ttccgcatgt gctggaaata tcccctgaat ccgccgctgc
     7381 attcaggcaa atttacgctg attacgaact gcttccccct ttccaacagc tcgagcgagg
     7441 tagctatcac cttgctgata atgaacgtaa tactcacgaa ctgacacgct ggcaaggacg
     7501 gctttgccag gccggacgca ttgtcgggct ggaacgcaga ggctggcaac gtctggaaga
     7561 aagcggcagc gtttatgcga tgcgcaaaac aacccctcat ggcgatctcg aacttgaaac
     7621 ggaacctttt tcattaattt atggtgaaac ggggtatggc gaccagctac cggttgaaag
     7681 cgtaaaaatc acctcgccag atgatcgcta cggtaaacaa tcctcactca ctttctccat
     7741 gctcgacgac atcaccgcca gcgagctgat taacgacatc gaatcactgt ttgattaaaa
     7801 ggatttaaca atgaaaacct atatttatca ggacgaaaaa tcacataaat tctgggcggt
     7861 ggagcagcag gaaaacgagc tgcatattac ctggggtaaa gtcggtagta acgggcaaag
     7921 tcaggtaaaa agctttgctg atgctacggc ggcggaaaaa gcggaactga agctgattgc
     7981 ggagaaggtg aagaaggggt atgtggagca agtagacgct aacgctcact cacatgatgc
     8041 ctcagcaaag aaagtcctgg tcacagagga aaaagcctca ataaatcgcc aggcagcaac
     8101 taatggttta ccctggctgg cagacaacga tcccattatt cttcccccga atattgcccg
     8161 ccatgccctc agccaccgcc tttggccggg agagccagta aaaaaaccaa ccaaacccga
     8221 gaaacaaaat ttaatttccc gattggcaaa tcgtacttat cattattata aaaatctgac
     8281 tttcgattac agctcatgct ctgatgaatg ggctgatgcg attagccagg caatcagctt
     8341 aattaaggcc caaaagactg agatacccgc gcgtactatg gcggtactta cttttctgga
     8401 aatatgtttt tgtcagggct ggaatgatga aacctcatcc gagttgctgg atgaaattgt
     8461 aagcaccttc ggtatcgaat acgcaactga aactgtcatc ttctggtggc aaattgattt
     8521 cgattttgac tataaagatg aacatttgac tttctttatt aattatgcag atagcagcaa
     8581 ggacagttat agaaggaata acgacaaatt tagtttacgt ctgcgtaaac atttatcact
     8641 agcagaagaa gaagtatggc aaaattgcat tgcaaaatta ctcgttgcgt taccagatat
     8701 atcactctat cgccaaccgt taattgccat tttaatgcca gaaatacccg aagtggccca
     8761 tgagatcgta catcgtttac accaggtcgc agatgttcca caacttgaaa tgttgaaact
     8821 ggtagcgaca gatccattca cgctcgaaat actggagaac tatcaataca ttgatgtatt
     8881 taacacctat ggtgccagtt ggtcagctac ggtattacgg gaacaaggta tagcggcact
     8941 ggctcgcctg gcttcctatg ctgaagaaga taattgcggt gacgtactga aatgtattaa
     9001 tcatccgttg gccattacgg cactcatcga agccgccgat actaataaac gctgttatga
     9061 acgactaata aaaagtgccg aaaattttcc ttctgccacg atagcggcat tagccgaagc
     9121 attggtaaaa aaagatgata aacgcaaaca tacgttgctg gtttcattac ttaatgcaaa
     9181 tcctttattg gtcgaaaaat tagtcatcgg attatcgtcg caagcggcaa atttattgca
     9241 acaatgtcag caattgctga atagtcgcaa tgaagatttt gcaaactcag aaattctacc
     9301 aatggtacta gtctcgccgc cctgggaaac tcaaaataaa aataacgaca acagtcctgt
     9361 gggtaaatac ccggcgaaaa tccccccttt accgttgttt tatcaacctc cattatggac
     9421 tcgcccaaaa ctcaaaaata gcagcaaggt actgccagat aacgccctcc tccacctcgg
     9481 tgaaatgctc cgcttccctc aggaagaggc gctgtatccg ggattattgc aggtgaaaga
     9541 tgcctgtacc gccgactcac tggctgaatt tgcctgggat ctgtttaccg cctggctgac
     9601 tacgggtgca ccatcgaaag aaagctgggc gttcactgcg ttaggtgttc tcggtaacga
     9661 cgacaccgct cgcaaactga cgccattaat acgcgcctgg cctggcgaat ctcagcataa
     9721 acgcgccacc gttgggttgg atattctcgc cgctatcggt agtgatatcg ctctgatgca
     9781 gcttaacggc attgctcaga aactgaaatt caaagcttta caggagcggg cgaaagagaa
     9841 aattgccgac attgctgaaa gtcgcgaact gacagtggag gagcttgaag atcggttagc
     9901 accggatctc agtctggatg ataacggttc gttgctgctg gatttcggcc cacgccagtt
     9961 caccgtcagc tttgatgaaa ccttaaaacc gtttgtgcgt gacgcttccg gcagccgtct
    10021 gaaagacctg cccaaaccaa acaaaagcga tgatgaatca caggcgaacg atgctgttaa
    10081 ccgctacaaa ctgctgaaaa aagatgcgcg taccgtcgcc gcccagcagg tagcaaggct
    10141 ggaatccgcc atgtgcctgc gtcgccgctg gtcaccggaa aactttcagc tcttcctggt
    10201 tgagcatccg ctggttcgcc acttaacccg ccgtctgatt tggggcgttt atagcgccga
    10261 aaaccagctg caggcttgct ttcgcgtagc ggaagataac agttacagca ccgctgacga
    10321 tgatcttttc accctgccgg aaggcgatat ctctattggt attcctcacg ttctggaaat
    10381 atcaccgacg gatgctgccg cctttggtca gctttttgcc gactacgaac tgttaccacc
    10441 gttccgccag cttgaccgta acagttacgc cctgacagaa gccgagcgca acgccagcga
    10501 actgacccgc tgggcaggca gaaaatgccc gagcggtcgg gtaatggggc tggcgaataa
    10561 aggctggata aagggcgaac cacaggatgg aggctggatc ggctggatga tcaaacctct
    10621 gggccgctgg tcgttaatca tggaaatcga tgaaggcttt gcggtaggca tgtcgccagc
    10681 cgaactgagt gccgagcaac tcttaagcaa gctgtggcta tgggaaggca aagcggaaag
    10741 ctatggctgg gggagtaatt caacacagga agcgcagctc tccgtactcg ataccatcac
    10801 cgccagcgag ctaattaacg atattgaagc cctgtttgaa taaggaaaga gcatggacaa
    10861 ggaattaccg tggctggctg ataacgccca actggaactg aaatataaaa agggtaaaac
    10921 accactcagc catcgcaatt ggccgggcga accagtgcca gttatcactg aaagcatcat
    10981 tcagacactg ggtgatgaat tgctacaaaa agctgagaag aaaaaaaaca ttgtctggcg
    11041 ttatgaaaat ttttcactgg agtggcagtc cgccatcacg caggccatca acttgatcgg
    11101 cgaacacaaa ccctcaatcc cggcacggac aatggcggcg ctagcctgta tcgcgcaaaa
    11161 tgacagccaa cagttgctcg acgaaatcgt ccaacaagag gggctggaat atgcgactga
    11221 ggtggtgatt gcacgccagt ttattgcacg gtgttatgag agtgatcctc tggtagtcac
    11281 attgcagtat cagaacgagg actatggcta tggttatcgc tcagaaacct ataacgaatt
    11341 cgatctccga ctgcgtaaac acctctctct ggcagaggaa agctgctggc agcgttgcgc
    11401 cgacaaactc attgccgcac tgccaggaat aaccaaagtt cgccgtcctt ttattgcgct
    11461 gatcctcccg gaaaaacctg aaatagccaa tgaactcgcc agtcttgaaa gctcccggtc
    11521 aagtctccat tcaaaggagt ggttaaaggt cgttgctact gataatacgg cggtaaaaaa
    11581 actcgaacga tactggggcc tggacgtatt tagcgatcgc gaagccagct acatgtcaca
    11641 agaaaaccgc ttcggctacg cggcctgcgc ctcccttttg cgcgaacaag gactggtagc
    11701 cattccgcgc ctcgcgatgt atgcccataa agaagattgc ggcagtctgt tggtacaaat
    11761 taaccatccg caggtcatcc acaccttgct actggtggct gataaaaaca aacccagcct
    11821 gcaacgtgtg gctaaataca gtaaaaactt cccccacgcg acgctcgcgg cactggcaga
    11881 actgctggcg ttaaaagaac caccaacccg ccctggttat ccaatcatcg aagacaaaaa
    11941 gctgcctgcc cagcaaaaag cacgcgatga atactggcgt acgctgttac aaacgctgac
    12001 ggcatcgcag ccacaactgg cagaagaggt gatgcagtgg ttaagtactc aagcccgggc
    12061 agtactgaat agttatttat tggcaccgcc caaaccggtt attgatggca ccgataacag
    12121 caatctgcct gaaatcctcg tttcaccacc gtggcgtagt aagaaaaaaa tgacagctcc
    12181 acgtcttgat ttggcaccgc tcgaattaac cccgcaagtt tactggcaac caggcgaaca
    12241 agagaggctt gcctccactg agtctgcccg ttatttcagc acggaatctc ttgcggaacg
    12301 catggaacaa aaaagtggac gagttgtatt acaggaactg gtttttgggg atgatgtatg
    12361 gctgtttctg aattatatac tccccggaaa actggatgct gcacgcaatt cactcattgt
    12421 tcagtggcat tactaccagg ggcgggttga agagatcctg aatggctgga actccccgga
    12481 agcacaatta gcagaacagg cgcttcgcag cggtcacgta gaagcgttaa ttaacatatg
    12541 ggaaaatgac aactactcac gttatcgtcc ggaaaagagt gtctggaacc tgtatttatt
    12601 ggcacagttg ccgcgtgaga tggctttgac cttctggctg cgtatcaatg agaaaaaaca
    12661 tctgttcgcg ggtgaggact attttctcag tatcctcgga ttggatgcgc taccaggtct
    12721 gctgttggct tttttacatc gtccaaaaga aacatttccg ttaattttaa attttggcgc
    12781 aacagaactg gccctgcccg ttgcccgcgt ctggcaccgt tttgcgggcc agcgtaatct
    12841 ggctcgccag tggattttac aatggccgga acatacggct actgcactta tccctcttgt
    12901 ctttatcaaa cccagcgata atagcgaagc cgcattactt gccctgcgtt tactgtacga
    12961 acagggacat ggcgagttgc tgcaaaccgt ggcaaaccgc tggcaacgcg ctgatgtatg
    13021 gcctgccctg gagcagttgc ttaaacaggg tccaatggac atttacccgg cacgcattcc
    13081 aaaagcccct gatttctggc atccgcaaat gtggtccagg ccgcgcctta tcactaataa
    13141 tcaacctgtt accgatgacg ctctggaaat tatcggcgaa atgctgcgct ttacccaggg
    13201 gggacgtttt tatagcggtc tggaacaact gaaaacgttt tgccagccac aaacgctggc
    13261 cgcttttgcc tgggatctct tcactgcgtg gcaacaagct ggtgcccccg caaaagacaa
    13321 ctgggcattt ctggcgttaa gtctctttgg tgacgaaagc acggctcggg atctgacgac
    13381 acagatcctc gcctggccac aagaaggcaa atctgcccgt gctgtcagcg gcctgaacat
    13441 ccttaccctg atgaataatg atatggcgct gatgcagctg catcatatat cgcaacgggc
    13501 taaatcccgc cccttacgtg ataacgcggc ggaatttctt caggtggtcg cagaaaatcg
    13561 cgggctaagc caggaagagc tagcggacag attagtccca atcctgggcc ttgatgatcc
    13621 gcaggcgttg agttttgatt ttggtccccg gcagtttacc gttcgcttcg atgaaaacct
    13681 caacccggtt atctttgatc agcaaaacgt tcgccagaaa agtgttcccc ggttgcgtgc
    13741 cgatgacgat caactgaaag cgcccgaggc actggcccga ctaaaagggc taaaaaaaga
    13801 tgctactcag gtgagcaaaa acctgctccc gcgtcttgaa gctgccctac gtaccacccg
    13861 acgctggtcg ctggcagatt ttcattctct gtttgttaat catcccttta cccgcctggt
    13921 tacccagcga ttaatatggg ggggttatcc ggcaaatgaa ccgcgtcgtt tactcaacgc
    13981 ctttcgtgtg gccgcagagg gggagttctg caatgcgcag gatgagccca ttgaccttcc
    14041 tgcggatgct ctgattggca ttgcccaccc gttagaaatg acagcagaaa tgcgcagtga
    14101 atttgcacag ctttttgccg attacgaaat tatgccgcct tttcgccagt tgtcgcgccg
    14161 cacggtgctg ctcacacctg acgagtcaac cagtaacagc ctgactcgct gggaaggtaa
    14221 atccgctacc gttgggcaac ttatgggaat gcgatacaaa ggctgggaat caggctatga
    14281 ggacgcattt gtctatgacc tgggtgagta ccggctggtc cttaagtttt cacccggttt
    14341 taaccactac aatgttgata gcaaagcgct aatgagcttc cgttctcttc gagtgtaccg
    14401 tgacaataaa tccgtcactt ttgccgaact tgatgtgttt gatttgagtg agacgttaag
    14461 cgcacctgac gtcattttcc attaaaacac agcgggcagt gatgcaactg cctgttatta
    14521 acacgactta ccagcgggga aagcgatgat cgtgcaaaaa gagctggttg ctatttacga
    14581 ttatgaggtc cctgtacctg aagatccgtt ttccttcaga cttgagatcc ataaatgctc
    14641 tgaattattt acaggttccg tctatcgacg ggagcgattc cggctacatc caacatttca
    14701 tcaacgtgat cgagaagatg ctgacccgct aataaatgat gcgttgattt atataagaga
    14761 tgagtgtatt gatgagcgga aattacgagg taaatcacct gaaactgtaa tagcaatttt
    14821 taatcgtgaa ctacagaata tattcaacca agaaatagaa taatatactc tagataattc
    14881 aagtcgcagc acatgcaact tgaagtatga cgagtatagc tttattaaag ggggaagcgg
    14941 ttttccccct tagatgcgtt tcaatttgcc cgttacttca acaaatcaat caaccgtaac
    15001 agagccacta ataactccag tacggcaatg agcacgatta acttttgtgt cagcgtgtcc
    15061 atccgctctc cttattaagg agcgcaacac cagcacctta cccttacact gcctgtcgat
    15121 actggtttac gtatgttggt acgaaacgtt gctctccgga tcagggcacc gcctgcggca
    15181 ccacccgtcc agcagccagg acttaaaatt ggtccgatcc ggcacaacct cccaacgggc
    15241 aggattataa atctcattca ctgaattgca atggagtgtg gatatggtta taatttattc
    15301 gttggtacga aatgttgcac aatcagcaag ttagcgtcac tgtagtaaat aaaagaacct
    15361 gcctcaccag caggtttttt tattttctgc gatctacttt ccagatattt tttgccaata
    15421 gcccaccata aatattcaca tcccggtcaa atggtgcgtt gaatataaaa tcgatgaact
    15481 tataaataaa catgagttat atgatgaatt aataaaaac
//
LOCUS       DAZFFG010000105        15340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFFG010000105 DAZFFG010000000
VERSION     DAZFFG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.105"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(80..1231)
                     /gene="argE"
                     /locus_tag="ACHJ7J_003777"
     CDS             complement(80..1231)
                     /gene="argE"
                     /locus_tag="ACHJ7J_003777"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO5001955.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            1385..2389
                     /gene="argC"
                     /locus_tag="ACHJ7J_003778"
     CDS             1385..2389
                     /gene="argC"
                     /locus_tag="ACHJ7J_003778"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO5001956.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWSGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKVAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            2397..3173
                     /gene="argB"
                     /locus_tag="ACHJ7J_003779"
     CDS             2397..3173
                     /gene="argB"
                     /locus_tag="ACHJ7J_003779"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO5001957.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            3234..4607
                     /gene="argH"
                     /locus_tag="ACHJ7J_003780"
     CDS             3234..4607
                     /gene="argH"
                     /locus_tag="ACHJ7J_003780"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO5001958.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     SSATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSERVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            4874..5791
                     /gene="oxyR"
                     /locus_tag="ACHJ7J_003781"
     CDS             4874..5791
                     /gene="oxyR"
                     /locus_tag="ACHJ7J_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO5001959.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(5774..7174)
                     /gene="sthA"
                     /locus_tag="ACHJ7J_003782"
     CDS             complement(5774..7174)
                     /gene="sthA"
                     /locus_tag="ACHJ7J_003782"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO5001960.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            7504..8670
                     /locus_tag="ACHJ7J_003783"
     CDS             7504..8670
                     /locus_tag="ACHJ7J_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO5001961.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKLWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            8713..10029
                     /locus_tag="ACHJ7J_003784"
     CDS             8713..10029
                     /locus_tag="ACHJ7J_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO5001962.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYATAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            10133..10783
                     /gene="fabR"
                     /locus_tag="ACHJ7J_003785"
     CDS             10133..10783
                     /gene="fabR"
                     /locus_tag="ACHJ7J_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO5001963.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            10783..11142
                     /gene="yijD"
                     /locus_tag="ACHJ7J_003786"
     CDS             10783..11142
                     /gene="yijD"
                     /locus_tag="ACHJ7J_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO5001964.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(11182..12282)
                     /gene="trmA"
                     /locus_tag="ACHJ7J_003787"
     CDS             complement(11182..12282)
                     /gene="trmA"
                     /locus_tag="ACHJ7J_003787"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO5001965.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      12396..12586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12651..14495
                     /gene="btuB"
                     /locus_tag="ACHJ7J_003788"
     CDS             12651..14495
                     /gene="btuB"
                     /locus_tag="ACHJ7J_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO5001966.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     SSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            14440..15297
                     /gene="murI"
                     /locus_tag="ACHJ7J_003789"
     CDS             14440..15297
                     /gene="murI"
                     /locus_tag="ACHJ7J_003789"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO5001967.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         3512 a         3686 c         4258 g         3884 t
ORIGIN      
        1 atcgcatccg acattattgc ggcactggag tttggcaaca gtgccggatg cggcgcaagc
       61 gccttattcg gcctacgttt taatgccagc aaaaatggtg aatcacctgg gtgatcagtt
      121 cgcgggtggg cttgataaac ctcgtctcca gatattcatc aggctgatgg gcttgattga
      181 ttgaacccgg ccccagaacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac
      241 agtaattcac cacttcggtt tttgctccga gcaatttctc aaccacttca accagttgat
      301 gattcggtgg gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac
      361 ccggccagcg ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga
      421 gtgtcatgcc aggcagcgga cgaatatcca tatgcaactc acagcaagcg caaatacggt
      481 tagaagcgtc gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaacg
      541 cgtcgtagtg ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt
      601 cgtgcatcag ttcgatagcg ttaactccgc gtgctggatc gctggagtgg cccgactggc
      661 cctgaatacg gatggcgtta gagatatgac ctttatgtgc gcgtaccggt tgtagtgacg
      721 tcggttcgcc aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg
      781 ctccggccat actggtttct tcatcagcag tagccagaat gtagagcggt tttttcagtt
      841 tcgtgacgtc gacatcgcgt agcgcatcaa ggataaacgc aaaaaagcct ttcatgtcgg
      901 cggtgcctaa gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc
      961 agcgaccgtc atcaaatggt accgtatcgg tatgccccgc cagcaacaag ccgccagccc
     1021 cctgtccggt acttgccagc atattgaatt tgttgcgagt tcctggcaca ggctgcactt
     1081 ccacattgaa gcctaaatct ttaaaccagt ccgccagcag agtgattaaa tctgcattgc
     1141 tttgatcgag tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa
     1201 tctcgataaa tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat
     1261 caatattcat gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac
     1321 cagccgtaag gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc
     1381 cccgatgttg aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac
     1441 ctatgtaaat cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa
     1501 tgatgcggga aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgagctgcc
     1561 gttgcagccg atgtcggata tcagcgagtt tagcccaggg gtggacgtgg tgtttctcgc
     1621 caccgcccat gaagttagcc acgatttagc gccgcagttt cttgaagcgg gctgcgtggt
     1681 gttcgacctt tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg
     1741 ctttacccat caatacccgg aactgctgga acaggcagcc tacggtctgg cggagtggag
     1801 cggtaataaa ttaaaagaag cgaatttgat tgcggtgcca ggctgttatc cgacggcggc
     1861 acagctggcg ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt
     1921 gatcaacgcc accagcggcg tgagcggtgc aggacgtaag gtagcgattt caaatagttt
     1981 ttgtgaagtt agcctgcaac cgtatggcgt ctttactcat cgccatcaac cagagatcgc
     2041 cacacacctc ggtgctgacg ttatcttcac cccgcatctg ggcaatttcc cacgcggcat
     2101 tctcgaaacc attacctgcc gcctgaaacc gggtgtgacc caggcgcaag tcgcgcaagt
     2161 gttacagcag gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc
     2221 gctgaaaaat gtcgttgggc tgccattttg cgatatcggg tttgccgttc agggcgagca
     2281 tttgattatt gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca
     2341 gtgcgccaat attcgtttcg gctatgcgga aacgcagtct cttatttaag ggtgcaatga
     2401 tgaatccatt aattatcaaa ctgggtggcg tactgctgga tagtgaagag gcgctggagc
     2461 gtctgtttag cgcactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtcc
     2521 acggcggcgg ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga
     2581 aaaatggcct gcgggtgacg cctgcggatc agatagacat tatcactgga gcactggcgg
     2641 gaacggcaaa taaaaccctg ctggcatggg cgaagaaaca tcagattgcg gccgtaggtt
     2701 tgtttctcgg tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggccatg
     2761 ttggactggc gcagccaggt tcgcctaagc ttatcaacac cttgctggag aacggttatc
     2821 tgccggtggt cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg
     2881 accaggcggc aacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctccgacg
     2941 ttagtggcat tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag
     3001 aacaactgat tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc
     3061 tggatgctgc gcgcacgctg ggccgcccgg tggatatcgc ctcctggcgt catgcggagc
     3121 agcttccggc actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt
     3181 tggccggagg cgcagctttc cggcattgat tttcaaaata aggaaacaga gttatggcac
     3241 tttggggcgg gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac
     3301 tgcgctttga ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag
     3361 ccctggtaac ggtcggcgtg ttaaccgcag aagagcaggc gcaactggaa gaggcgctga
     3421 acgtgctgct ggaagatgtt cgcgccaggc cacaacaaat cctcgaaagc gacgccgaag
     3481 atatccatag ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc
     3541 tgcataccgg gcgtagtcgt aatgatcagg tagcgactga cctgaaactg tggtgcaaag
     3601 ataccgttag cgagttactg acggctaacc gacagctgca atcggcgctg gtggaaaccg
     3661 cacaaaacaa tcaggacgcg gtcatgccag gttacactca cctgcaacgc gcccagccgg
     3721 tgacgttcgc gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt
     3781 tgcaggatgc gcttaagcgt ctggatgtca gcccattagg ctgtggcgcg ctggcgggaa
     3841 cggcctatga aatcgaccgt gaacagttag caggctggct gggcttttct tcggcgaccc
     3901 gtaacagtct cgacagcgtt tctgaccgtg accacgtgct ggaactgctg tcggctgccg
     3961 caatcggcat ggtgcatctt tcgcgttttg ctgaagatct gattttcttt aacaccggcg
     4021 aagcggggtt tgtggagctt tctgaacgcg tgacttccgg ttcatcatta atgccgcaga
     4081 agaaaaaccc ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggcgcgt
     4141 taaccggcat gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg
     4201 aagacaaaga aggtctgttc gacgcgctcg atacctggct ggactgcctg catatggcgg
     4261 cgctggtgct ggacggcatc caggtgaaac gtccacgttg ccaggaagcg gctcagcagg
     4321 gttacgccaa cgccaccgaa ctggcggatt acctggtggc gaaaggcgta cctttccgcg
     4381 aggcgcacca tattgttggt gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg
     4441 aagaactgcc gctcgacgag ttgcagaaat tcagcccggt gattggcgaa gatgtctatc
     4501 cgattttgtc gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc
     4561 agcaggtggc gcaggcgatt gcttttgcaa gggcgcggtt ggggtaagaa catttatatg
     4621 tacaaatttg agcctggctt atcgccgggc tttttcatgg caaaaaaaag cggatcctgg
     4681 agatccgcaa aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc
     4741 agtgacttca agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa
     4801 tgcttgatag ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcga
     4861 tggaggatgg ataatgaata ttcgtgatct tgagtacctg gtggcattgg ctgaacaccg
     4921 ccattttcgg cgtgcggcag attcctgcca cgttagccag ccgacgctta gcgggcaaat
     4981 tcgtaagctg gaagatgagc tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt
     5041 gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt accgtgctgc gtgaggtgaa
     5101 agtccttaaa gagatggcaa gccagcaggg cgagacgatg tccggaccgc tgcatattgg
     5161 tttgattccc acggttggac cgtacctgct accgcatatt atcccgatgc tgcaccagac
     5221 atttccaaag ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact
     5281 ggacagtggc aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat
     5341 tgaagtgccg ttgtttgatg agccaatgtt gctggctatc tatgaagacc acccgtgggc
     5401 gaaccgcgaa tgcgtaccga tggccgatct ggctggggaa aaactgctga tgctggaaga
     5461 tggtcactgt ttgcgcgatc aggcaatggg tttttgcttt gaagccgggg cggatgaaga
     5521 tacacacttc cgcgcgacca gcctggagac gctgcgcaac atggtggcgg caggtagcgg
     5581 gatcacttta ctgccagcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta
     5641 tctgccgtgc attaagccgg aaccacgccg cactattggc ctggtttatc gtcctggctc
     5701 accgctgcgc agccgctatg agcagctggc agaggccatc cgcgcaagaa tggatggcca
     5761 tttcgataaa gtgttaaaac aggcggttta aaccgtttaa cgcagctacc cgataggctt
     5821 ccgccatcgt cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac
     5881 ctttctgttc cataatcgcc tgaccgatat gaataatttc ggcagcgcgc tcgccaaagc
     5941 agtgaatgcc cagaatctct tttgtttccc gatggaacaa aattttcagc gtgcccacgt
     6001 tcatgccgac gatttgtgca cgtgccagat gtttgaactg ggcgcggccc acttcatatg
     6061 gcactttcat cgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg
     6121 tgtaaatgcc ggtagggata tcttcaatca gatgcgcggt ggcttcgcct tttaccagcg
     6181 cctgcgcggc aatgcgcccc tggtcatagg ccgctgatgc caggctcgga taaccaatca
     6241 cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata catgctgttg accttcagct
     6301 gtccgcgact gtcagtttcc agcccaatgt tctgtaatgc cagcgaatcg gtattaccgg
     6361 tgcggccgtt ggcatagagc agacagtcgg ctttcagttt tttacccgac ttcagatgca
     6421 tgattacacc gtcgtcacag ccttcgatct tctcgtactc ttcgttgtga cgaatcacta
     6481 cgccactgtt ccagaagtga taggagagag aatctgacat ctcttgatcg agaaatgcca
     6541 gcaggcgatc gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg
     6601 catattcaca gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca
     6661 tgctgagaat tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat
     6721 gatatggacg agagccgcag gcaataacaa atttttcagc ggttagtgtt tcaacgctgc
     6781 cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata
     6841 tttcacagtg attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt
     6901 tatcggcatg gttaaggata tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt
     6961 atagtgggtt ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg
     7021 ggatggtgcc ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga
     7081 cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta
     7141 ttactatggc atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat
     7201 aaaagcaaca gaatggtaac attttatcgc gggtaagcca attgatcccc gtcattaatc
     7261 tggctatagc gtgagcggcc tttgctttgt ctgcctcctt cttaacccat ttattcacta
     7321 ttaatcgctt tttattatgg ctgatagttt tatcttgccg aagcgtgctt ttttgttatt
     7381 gtctttatga aatcttctta taaaacaaac atataaatat tttatattga tgggttattt
     7441 gtatttatac catcaataaa atccgttaat tagcctgttt ataagtgaat aaaggaatag
     7501 ccgatgatcg aacactatat tcgagggttt gaggaagaac tgcgtgagat ccgccatcag
     7561 atccacgaaa acccggagct gggattacaa gaatttaaaa ccagtgcttt ggttgcggaa
     7621 aagctgcgtc agtggggcta tgaagtagag caaggactgg cgacgacggg gatagtggcc
     7681 acgttaaaag tgggcgacgg agagaaaagt attggcctgc gggcggatat ggatgcgctg
     7741 ccgatttatg aaaatagtgg caagctctgg gccagtaaac atcctggttt aatgcacgcc
     7801 tgcgggcatg acgggcatac caccatactg ctgggggccg cgcgttattt tgccgaaacc
     7861 cgccgtttta acggcacgtt acgtcttatc ttccagcctg cggaagagat gattaatggc
     7921 ggtgagatca tggttaaaga ggggcttttt gatcatttcc cctgcgatgt cattttcggc
     7981 atgcataaca tgcccggtct gccggtgggt aagttttatt tccagccagg ggcactgatg
     8041 gcgtcaatgg atcagttcca tattacagtt cgtggttgtg gcggacacgg tgcgatcccg
     8101 cacaaagcca tcgatccggt gctggttgcc gcacatatca ccaccgcatt acaaagcatt
     8161 gtgtcacgca atgtcgatcc gctggaagcg gcggtaatta ccgtcggcag cattgttgcg
     8221 ggtgaggcgg ctaacgtcat tcctgacagt gctgagatga aaatcagcgt ccgctctctt
     8281 agccgcgata cccgacaact tcttctgacc cgtattcctg ctctggcaca agcccaggct
     8341 gccagctttg gcgctacggc tgaggttacg catgttaacg gtacaccagt attagtaaat
     8401 gacgaggaga tggcgcgctt cgcctggcag gtagcgtgta aaacgtttgg tgaagatcgg
     8461 gccgagtttg gcatcaagcc tctgatgggc agtgaagatt tctctttcat gctggaagcc
     8521 caacccaaag gcggtttcct gttattcggt aatggcgatg tcggggaagg ttcctgcatg
     8581 gtgcataacc cgggttacga cttcaacgat gcaagtctgg tcccggcaag cagctactgg
     8641 ggcgcactgg tagaagcctg gctgcaataa ataaaacaac acacaacaac actttttggc
     8701 aaggaacatg gaatgacggc gatcgtaaat gaaaccgtga gtgtgaagaa aaagcccagc
     8761 ggcaggcggg tgatttttgc ctcggcgttt ggcaatgcgc tcgaattttt tgattttggc
     8821 gtttacaact tttttgtggt ctatatcagt acacttttct tcccacccag cgctgatcac
     8881 aatgtcgcgc tgttactggc atttgcaacg tttggcgtga gtttttttat gcgcccgctt
     8941 ggtggaatta tcgtcggggc ctgggccgat cgtttcggac gcaaacccgc gatggtgttc
     9001 actattgccc tgatgagcct cggcacgttg atgattggca ttgcgccgac gtatgcgacg
     9061 gcgggctatt ggggaacggc aactctggta cttgctcgtt tgattcaggg tgttgcagca
     9121 ggcggtgaag ttggggcttc aatgtcgcta ctggtggagt cagctccggc aaatcgtcgc
     9181 ggtttttaca gcagttggtc actggcgact caaggcctgg caacaacttt tggaggtgtt
     9241 gtcgctttag gtttaagtgc atggctgccc tttgcgaccg gttcagaaac cgtcatggct
     9301 gaatggggct ggcgtgtgcc gttttttatt ggcgtgttat tagcgcctgt cggctgttgg
     9361 ttgcgtttga gtctggaaaa tgacgtacct gagcctgtgc gcaataaaaa agctgctacc
     9421 agcgaaagcg cgttttctct gctcctgcaa cataaggcga ctatcgttaa tggcgtttta
     9481 ctcgctattg gtagcacggt cgcaacctat atctccctct tttattacgg cacctgggcg
     9541 gcgaagtatc tggcgatgcc tcaacattat tctcatgcag cgatgttact ggcgggcgtt
     9601 attacgtttg tgggtgcgct gcttgtgggg atgttgtgcg attcggtcgg gcgtaaaaag
     9661 ctgattttaa tctcccgtgt gatggtgctg atctgtagct ggccgtcatt ctggctgttg
     9721 gtgaattacc caagccctgg catgttgctg acggtggttt tcgtgatggt cagctttacc
     9781 acgcttggcg gtgtgccagt gatgttgttg atctctgaac tgttgccgaa acggattcgg
     9841 gcattgggtt ttgcgctggt ttatagcatt ggtgtggcaa tctttggcgg ttttgctcaa
     9901 tatttcgcta cgcaatccat cgtgttgctg gatagtttga cggctccggc gtggtatctg
     9961 ggtggaggca cgttgctgtc gatgttggcg ttgttgtacg taaaagaacc ggccaaagaa
    10021 ttgcagtaaa tatgttttat tgcgttaccg ttcattcaca atactggagc aatccagtat
    10081 gttcattctc tggtatagtg ccagcagtac ttttggcaag gattcagaca tcgtgatggg
    10141 cgtaagagcg caacaaaaag aaaaaacccg ccgttcgctg gtggaagccg catttagcca
    10201 attaagtgct gaacgcagct tcgccagcct gagtttgcgt gaagtggcgc gtgaagcggg
    10261 cattgctccc acctcttttt atcggcattt ccgcgacgta gacgaactgg gtctgaccat
    10321 ggttgatgag agcggtttaa tgctacgcca actcatgcgc caggcgcgtc agcgtatcgc
    10381 caaaggcggg agtgtgatcc gcacctcggt ctccacattt atggagttca tcggtaataa
    10441 tcctaacgcc ttccggttat tattgcggga acgctccggc acctccgctg cgtttcgtgc
    10501 cgccgttgcg cgtgaaattc agcacttcat tgcggaactt gcggactatc tggaactcga
    10561 aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa gcaatggtga caattgtctt
    10621 cagtgcgggt gccgaggcgt tggacgtcgg cgtcgaacaa cgtcggcaat tagaagagcg
    10681 actggtactg caactgcgaa tgatttcgaa aggggcttat tactggtatc gccgtgaaca
    10741 agagaaaacc gcaattattc cgggaaatgt gaaggacgag taatgaaaca agcaaatcaa
    10801 gatagaggta cgctgctgct ggcgttggtt gctggcttat cgattaatgg tactttcgca
    10861 gcgctgttta gctccattgt gccattttct gtattcccga ttatttccct ggtgctgacg
    10921 gtttactgcc tgcatcaacg ttatcttaat cgcactatgc cggtaggctt gccgggtctg
    10981 gcagctgcct gttttattct cggcgtactg ctgtacagca cggtagttcg tgcggaatat
    11041 ccggatatcg gctctaactt cttcccggca gtactctccg tcattatggt gttctggatt
    11101 ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgagt aatcggtatc atgccggatg
    11161 ctggcgcatc cggcatgggt tttacttcgc ggtcagtaat acgccgcact ccatatggtg
    11221 cgtgtagggg aactgatcaa acagagccag acgttcgacc ttgtgcgtct ggcttaatgt
    11281 ttccagattc ttgcataacg tttccggatt gcaggagata tacagaatac gtggatacgc
    11341 ctgcaccatt ttctcggttt cactgtccag accgctgcgc ggtgggtcga caaaaatggt
    11401 ttcgcactga taactcttta agtcgatccc ttgcaggcgg ttaaactcgc gcacgccatt
    11461 catcgcctga gtaaattctt ctgccgccat acgaataatt tgtacgttat caatatggtt
    11521 agcggcaata ttgtattgcg cggcagcgac tgacggcttg gcgatttcgg tggctaatac
    11581 ccgatcaaaa ttgcgcgcca gggctaatga gaagttaccg ttgccgcagt acagctccag
    11641 taaatcgcct tttgagcctt tggttacgtc cagcgcccat tccagcatct gaatattcat
    11701 cgccgcgttc ggctgggtaa agctgttttc tacctgacgg tagatcatct ctttccctgc
    11761 gaccggcaga cgttcgtcga tgtaatcttg atccagcgcg attttggttt tcgttgcccg
    11821 accaatcaga tgcacattca gattctgtgc tcgcagtgca tcgcgcaggg cttccgcttc
    11881 ctgacgccac tcatcatcca gctttttatg gtacagcagg gaaaccaccg cctgattact
    11941 cagcgtggtg aggtaatcaa tctggaacaa cttgtggcgc agaacgggat tattacgcac
    12001 acccgcaatc atcgccgtca tcaactggtt gatgagttca ctggcggcgg ggaagctatc
    12061 cacgcggata cggcttttgg tttgttgatc gaaaatgatg tgatacaggt catcgccatc
    12121 gtgccagatg cggaactccg cacgcatccg gtaatgactg accggcgagc gaaacacttc
    12181 cggaaccagg tcagaaaacg gtgccatcat actttgcaaa cgtaccactt tttcggctaa
    12241 ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc atgatgtatc ctgaaaaatt
    12301 aaagtacgcg gcgattgtag ggattgctca tcagatgtcc agatcttgat gaattcctat
    12361 ttgtgagcta cgtctggaca gtaacttgtt acaacctgta gcatccactt gccggtcctg
    12421 tgagttaata gggaatccag tgcgaatctg gagctgacgc gcagcggtaa ggaaaggtgc
    12481 gatgattgcg ttatgcggac actgccattc ggtgggaagt catcatctct tagtatctta
    12541 gatacccctc caagcccgaa gacctgccgg ccaacgtcgc atctggttct catcatcgcg
    12601 taatattgat gaaacctgcg gcatccttct tctattgtgg atgctttaca atgattaaaa
    12661 aagcttcgct gctgacggcg tgttctgtca cagccttttc cgcttgggca caggatacca
    12721 gcccggatac tctcgtcgtt actgctaacc gttttgaaca gccgcgcagc actgtgcttg
    12781 caccaaccac cgttgtgacg cgtcaggata tcgaccgctg gcagtcgacc tcggttaatg
    12841 atgtgctgcg ccgtcttccg ggcgtcgata tcacccaaaa cggcggttca ggtcagctct
    12901 catctatttt tattcgcggt acaaatgcca gtcatgtgtt ggtgttaatt gatggcgtac
    12961 gcctgaatct ggcgggggtg agtggttctg ccgaccttag ccagttccct attgcgcttg
    13021 tccagcgtgt tgaatatatc cgtgggccgc gctccgccgt ttatggttcc gatgcaatag
    13081 gcggggtggt gaatatcatc acgacgcgcg atgaacccgg aacggaaatt tcagcagggt
    13141 ggggaagcaa tagttatcaa aactatgatg tctctacaca gcaacaactg ggggataaga
    13201 cacgagtaac gttgttgggc gattatgccc atactcatgg ttatgatgtt gttgcctatg
    13261 gtaataccgg aacgcaagcg cagacagata acgatggttt tttaagtaaa acgctttatg
    13321 gcgcgctgga gcataacttt actgatgcct ggagcggctt tgtgcgcggc tatggctatg
    13381 ataaccgtac caattatgac gcgtattatt ctcccggttc accgttggtc gatacccgta
    13441 aactctatag ccaaagttgg gacgccgggc tgcgctataa cggcgaactg attaaatcac
    13501 aactcattac cagctatagc catagcaaag attacaacta cgatccccat tatggtcgtt
    13561 atgattcgtc ggcgacgctc gatgagatga agcaatacac cgtccagtgg gcaaacaatg
    13621 tcatcgttgg tcacggtagt attggtgcgg gtgtcgactg gcagaaacag actacgacgc
    13681 cgggtacagg ttatgttgag gatggatatg atcaacgtaa taccggcatc tatctgaccg
    13741 ggctgcaaca agtcggcgat tttacctttg aaggcgcagc acgcagcgac gataactcac
    13801 agtttggtcg tcatggaacc tggcaaagca gcgccggttg ggaattcatc gaaggttatc
    13861 gcttcattgc ttcctacggg acatcttata aggcaccaaa tctggggcaa ctgtatggct
    13921 tctacggaaa tccgaatctg gacccggaga aaagcaaaca gtgggaaggc gcgtttgaag
    13981 gcttaaccgc tggggtgaac tggcgtattt ccggatatcg taacgatgtc agtgacttga
    14041 tcgattatga tgatcacacc ctgaaatatt acaacgaagg gaaagcgcgg attaagggcg
    14101 tcgaggcgac cgccaatttt gataccggac cactgacgca tactgtgagt tatgattatg
    14161 tcgatgcgcg caatgcgatt accgacacgc cgttgttacg ccgtgctaaa cagcaggtga
    14221 aataccagct cgactggcag ttgtatgact tcgactgggg tattacttat cagtatttag
    14281 gcactcgcta tgataaggat tactcatctt atccttatca aaccgttaaa atgggcggtg
    14341 tgagcttgtg ggatcttgcg gttgcatatc cggtcacctc tcacctgaca gttcgtggta
    14401 aaatagccaa cctgttcgac aaagattatg agacagtcta tggctaccaa actgcaggac
    14461 gggaatacac cttgtctggc agctacacct tctgaaccac gtcccaccgt gctggtgttt
    14521 gactccggcg tcggtgggtt gtcggtctat gacgagatcc ggcatctctt accggatctc
    14581 cattacattt atgctttcga taacgtcgct ttcccgtatg gcgaaaaaag cgaagcgttt
    14641 attgttgagc gagtggtggc aattgtcacc gcggtgcaag aacgttatcc ccttgcgctg
    14701 gctgtggtcg cttgcaacac cgccagtacc gtttcacttc ctgccttacg cgaaaagttc
    14761 gacttcccag ttgttggtgt cgttccggcg attaaacctg ctgcacgtct gacggcaaat
    14821 ggcattgtcg gattactggc aacccgcgga acagttaaac gttcttatac tcatgagctg
    14881 atcgcgcgtt tcgctaatga atgccagata gaaatgctgg gctcggcaga gatggttgag
    14941 ttggctgaag cgaagctgca tggcgaagat gtttctctgg atgcactaaa acgtatccta
    15001 cgcccgtggt taagaatgaa agagccgcca gataccgttg tattgggttg cacccatttc
    15061 cctctactac aagaagaact gttacaagtg ctgccagagg gaacccggct ggtggattct
    15121 ggcgcagcga ttgctcgccg aacggcctgg ttgttagaac atgaagcccc ggatgcaaaa
    15181 tctgccgatg cgaatattgc cttttgtatg gcaatgacgc cagaagctga acaattattg
    15241 cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt aggctgattt
    15301 ggttgaatgt tgcgcggttg gaaaattatt ttaaatttcc
//
LOCUS       DAZFFG010000106        15193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFFG010000106 DAZFFG010000000
VERSION     DAZFFG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.106"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(1..1714)
                     /locus_tag="ACHJ7J_003790"
                     /pseudo
     CDS             complement(1..1714)
                     /locus_tag="ACHJ7J_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2237..4165
                     /gene="thrS"
                     /locus_tag="ACHJ7J_003791"
     CDS             2237..4165
                     /gene="thrS"
                     /locus_tag="ACHJ7J_003791"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO5001968.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4169..4711
                     /gene="infC"
                     /locus_tag="ACHJ7J_003792"
     CDS             4169..4711
                     /gene="infC"
                     /locus_tag="ACHJ7J_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO5001969.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4808..5005
                     /gene="rpmI"
                     /locus_tag="ACHJ7J_003793"
     CDS             4808..5005
                     /gene="rpmI"
                     /locus_tag="ACHJ7J_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO5001970.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5058..5414
                     /gene="rplT"
                     /locus_tag="ACHJ7J_003794"
     CDS             5058..5414
                     /gene="rplT"
                     /locus_tag="ACHJ7J_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO5001971.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5531..5655
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5537..5581
                     /gene="pheM"
                     /locus_tag="ACHJ7J_003795"
     CDS             5537..5581
                     /gene="pheM"
                     /locus_tag="ACHJ7J_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO5001972.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5864..6847
                     /gene="pheS"
                     /locus_tag="ACHJ7J_003796"
     CDS             5864..6847
                     /gene="pheS"
                     /locus_tag="ACHJ7J_003796"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO5001973.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6862..9249
                     /gene="pheT"
                     /locus_tag="ACHJ7J_003797"
     CDS             6862..9249
                     /gene="pheT"
                     /locus_tag="ACHJ7J_003797"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO5001974.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9254..9553
                     /gene="ihfA"
                     /locus_tag="ACHJ7J_003798"
     CDS             9254..9553
                     /gene="ihfA"
                     /locus_tag="ACHJ7J_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO5001975.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9654..10634
                     /gene="btuC"
                     /locus_tag="ACHJ7J_003799"
     CDS             9654..10634
                     /gene="btuC"
                     /locus_tag="ACHJ7J_003799"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO5001976.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10697..11248
                     /gene="btuE"
                     /locus_tag="ACHJ7J_003800"
     CDS             10697..11248
                     /gene="btuE"
                     /locus_tag="ACHJ7J_003800"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO5001977.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11248..11997
                     /gene="btuD"
                     /locus_tag="ACHJ7J_003801"
     CDS             11248..11997
                     /gene="btuD"
                     /locus_tag="ACHJ7J_003801"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO5001978.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12075..12539
                     /gene="nlpC"
                     /locus_tag="ACHJ7J_003802"
     CDS             12075..12539
                     /gene="nlpC"
                     /locus_tag="ACHJ7J_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5001979.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12786..13499
                     /gene="ydiV"
                     /locus_tag="ACHJ7J_003803"
     CDS             12786..13499
                     /gene="ydiV"
                     /locus_tag="ACHJ7J_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO5001980.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13562..14998
                     /gene="selO"
                     /locus_tag="ACHJ7J_003804"
     CDS             13562..14998
                     /gene="selO"
                     /locus_tag="ACHJ7J_003804"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO5001981.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGPGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQQLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYCREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15002..>15193)
                     /gene="hemP"
                     /locus_tag="ACHJ7J_003805"
     CDS             complement(15002..>15193)
                     /gene="hemP"
                     /locus_tag="ACHJ7J_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO5001982.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
BASE COUNT         3848 a         3475 c         3990 g         3880 t
ORIGIN      
        1 ttttttgcaa aagtatccag cgtcgatata taagataaca tgacgtcttt attacccttg
       61 ctcatagcga tgtataaagc aggggttcca tgtactgttg cgccttttaa taaatctatg
      121 atgttaattt tgctgaggtt atatttaaca gcgattccat aaattttaga gaggaaccgt
      181 gtgacacaca acgggtgatt attttccatt gcagcaaata accctggttc accatcacgt
      241 gttttcatgg ctaaaatatc catcaccatt tttttgttta aatgcttttg tgctattgga
      301 gcggttaatg tattcagaaa gatatcgatt atctcatcga atccatacaa catggagata
      361 taaagtccag gtgtttcgtt agcactttta gtttgcagta atttaaccag attatcttca
      421 tgaatgatgt gattatctac cagtgactga atttcttgca taaatatttt cacgttgtca
      481 gcatgtccgt tcatcatagc caaaaataaa gcaggtgtac caacgctatt ctgcgcagtt
      541 attatttcaa tgatttcttt tcagggagta tgggcaactg tctgaataat gtaataagct
      601 gttgagtctg gcctgtggcc attattaaca acattgccga tggatgtata ggggcaaatg
      661 gcattcgtgt cgcgataccc gtgaaaatag cccctggaac tccctcaggt aataggggca
      721 gaatagcaat tgcaccgttg cgaacataca tatcagccca ggtctgagca tcgggatctg
      781 aactgatgaa cctatcaagc gagagaaagc cccgtgctgt cctgtgggtg cctctgacgg
      841 caacgttggt atcgttcggg tcgtaaacgg acacaacata atttaattgc ccatacgccg
      901 actgctttat tcgtagcctt actgacaaag tatgattgtc tatatataaa atcgcggaca
      961 tatgactgac attatttgcc atcattcgac agaagaaaat atctaaagcg aagccaaaat
     1021 ccttactcgc gataacgcaa cttcctgcag gaggatggcg aagtaatgct ctgaactccg
     1081 cgtcccggac taaggggaca tgctgctgta tggattcaac ggaggagtaa tgtttaatca
     1141 ggttgacttt gccttttcca tttctcaagg aatcaagggc atattgcaat gacagatggc
     1201 gacaaaaaat agcgtcagaa ggagattgca aaaaaaatgc actaccattg agatttactg
     1261 ttggttgtat agttttttct ctatgttttt ttggggacac tttcatgcaa tggttacgtt
     1321 tttcggcagt taactgttcc tgttcaatat aatccagtat gcttcgcaaa tggttagctt
     1381 taataggcgt ggtttccagg aataaggaaa tgatttcttt tttatagtgt aaagtaatga
     1441 gtgtattttc tgcatggatg ggtgaaatga caaagttaat gataaaaagg gcagtggaat
     1501 aacaattgtg tgatattttt cgacagcttg cggtaaattc ctgagtgaca cagtatgaca
     1561 gcatcgaact taagtcagag gtatcaagta gggtgataag ctggcgcgca aattgattaa
     1621 taggctctga tgaaggcgtt ggtaggtcgc tatcgatgtg cattctatga ggagacttat
     1681 aatgagttct gataatgatt tcgttttcac tcataatcta ttcgccttat atatgtctgg
     1741 aggttttgag gatgatgatt aattattatc gccagaacga aatgtgcacg aaaaattgct
     1801 ataagaattg tgtcactttt atcattgcga tatgaccact tcgtcattaa tctcatgatc
     1861 gtttaaatac taccgccaaa tcacgcaacg aactttgctg gattcagttt atgctgctgt
     1921 aaatccgctc gcagtaaacc tttcagacgc tccgtgatgt tatcagttgt tctttaagcg
     1981 ttttgctggt gtactcacta caaacgaatt gcgaatcgaa tcaatgtgaa acggaaaggt
     2041 acaatctccc tttttttagt gttaacatcg ctcaaccggg tggaaggtga gaggttaatg
     2101 tctttcattt gtgtacctga ttgcgaacca atttagcatt tgttggctag atggtttcgc
     2161 aatgaactgt taataaacaa atttttcttt gtatgtgatc tttcgtgtgg gtcaccactg
     2221 caaataagga tataaaatgc ctgttataac tcttcctgat ggcagccaac gccattacga
     2281 tcacgctgta agccccatgg atgttgcgct ggacattggt ccaggtctgg cgaaagcctg
     2341 tatcgcaggg cgcgttaatg gcgaactggt tgatgcttgc gatctgattg aaaacgacgc
     2401 acaactgtcg atcattaccg cgaaagacga agaaggtctg gagatcattc gtcactcctg
     2461 tgcgcacctg ttagggcacg cgattaaaca actttggccg cataccaaaa tggcaatcgg
     2521 cccggttatt gacaacggtt tttattacga cgttgatctt gaccgcacgt taacccagga
     2581 agatgtcgaa gcactcgaga agcggatgca tgagcttgct gagaaaaact acgacgtcat
     2641 taagaagaaa gtcagctggc acgaagcgcg tgaaactttc gccaaccgtg gggagagcta
     2701 caaagtctcc attcttgacg aaaacatcgc ccatgatgac aagccaggtc tgtacttcca
     2761 tgaagaatat gtcgatatgt gccgcggtcc gcacgtaccg aacatgcgtt tctgccatca
     2821 tttcaaacta atgaaaacgg caggggctta ctggcgtggc gacagcaaca acaaaatgtt
     2881 gcaacgtatt tacggtacgg cgtgggcaga caaaaaagcg cttaacgctt acctgcagcg
     2941 cctggaagaa gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg acctgtacca
     3001 tatgcaggaa gaagcgccgg gtatggtatt ctggcacaac gacggctgga ccatcttccg
     3061 tgaactggaa gtgtttgttc gttctaaact gaaagagtac cagtatcagg aagttaaagg
     3121 tccgttcatg atggaccgtg tcctgtggga aaaaaccggt cactgggaca actacaaaga
     3181 tgcaatgttc accacgtctt ctgagaaccg tgaatactgc attaagccga tgaactgccc
     3241 gggtcacgta caaattttca accaggggct gaagtcttat cgcgatctgc cgctgcgtat
     3301 ggccgagttt ggtagctgcc accgtaacga gccgtcaggt tcgctgcatg gcctgatgcg
     3361 cgtgcgtggt tttacccagg atgacgcgca tatcttctgt actgaagaac aaattcgcga
     3421 tgaagttaac ggatgtatcc gtttagtcta tgatatgtac agcacttttg gcttcgagaa
     3481 gatcgtcgtc aaactctcca ctcgtcctga aaaacgtatt ggcagtgacg aaatgtggga
     3541 tcgtgctgag gcggacctgg cggttgcgct ggaagaaaac aacatcccgt ttgaatatca
     3601 actgggtgaa ggcgctttct acggtccgaa aattgaattt accctgtatg actgcctcga
     3661 tcgtgcatgg cagtgcggta cagtacagct ggacttctcc ttgccgtctc gtctgagcgc
     3721 ctcctatgtg ggcgaagaca acgagcgtaa ggtaccggta atgattcacc gcgcaattct
     3781 tgggtcgatg gaacgtttca tcggtatcct gaccgaagaa ttcgctggtt tcttcccgac
     3841 ctggcttgcg ccggttcagg ttgttatcat gaatattacc gattcacagt ctgattacgt
     3901 taacgaattg acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag acttgagaaa
     3961 tgagaagatt ggctttaaaa tccgcgagca cactttgcgt cgcgtcccat atatgctggt
     4021 ctgtggtgat aaagaggtgg aatcaggcaa agttgccgtt cgcacccgcc gtggtaaaga
     4081 cctgggaagc atggacgtaa atgaagtgat cgagaagctg caacaagaga ttcgcagccg
     4141 cagtcttaaa caattggagg aataaggtat taaaggcgga aaacgagttc aaacggcgcg
     4201 ccctaaccgt atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag gtctggaagg
     4261 cgagcagctt ggtattgtga gtctgagaga agctctggag aaagcagaag aagccggagt
     4321 agacttagtc gagatcagcc ctaacgccga gccgccggtt tgtcgtataa tggattacgg
     4381 caaattcctc tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc aaaaagttat
     4441 ccaggttaag gaaattaaat tccgtcctgg tacagatgaa ggcgactatc aggtaaaact
     4501 ccgcagcctg attcgctttc tcgaagaggg tgataaagcc aaaatcacgc tgcgtttccg
     4561 cggtcgtgag atggcgcacc agcaaatcgg tatggaagtg cttaaccgcg tgaaagacga
     4621 tttgcaagaa ctggcagtgg tcgaatcctt cccaacgaag atcgaaggcc gccagatgat
     4681 catggtgctc gctcctaaga agaaacagta aggccttcaa gtagcactgt ctgtggagcc
     4741 ttcgggcttc acagactttg ttcgcctttg tttcgtttat taacaatgcg aagtggaagt
     4801 tattaaaatg ccaaaaatta agaccgtacg cggtgctgct aagcgcttca aaaaaaccgg
     4861 taaaggtggt tttaagcaca agcacgctaa cctgcgtcac attctgacca aaaaagcgac
     4921 caaacgtaaa cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc tgggcctggt
     4981 aatcgcgtgc ctgccgtacg cataagccgt taacgttttt aactttttaa ttagaatata
     5041 gatacaggag agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc gcacgtcaca
     5101 agaaaatttt gaaacaagct aaaggctact acggtgcgcg ttctcgcgta taccgcgttg
     5161 ccttccaggc tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt caacgtaagc
     5221 gtcagttccg tcaactgtgg attgcgcgta tcaacgcagc agcacgtcag aacggtattt
     5281 cttacagcaa attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac cgtaagatcc
     5341 tggctgatat cgcagtattc gacaaagtag cgttcaccgc tctggttgaa aaagcgaaag
     5401 cagctctggc ataagccagt tgaaagaggg agctagtctc cctcttttcg tttcaacgcc
     5461 atcaaaacat tgacttttat cgccgtagcc ttttcaataa aggtcttttg aagagtacca
     5521 aaaggtaacg caagcaatga atgctgctat tttccgcttc tttttttact ttagcacctg
     5581 aatccaggag gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa gcctcccagt
     5641 ggaggctttt tttgtatgcg cgttttgaaa aataaaaagc cagtctatca ggcgtaattg
     5701 tcgcagccag tctggatacg gccagtgcgg agaaaccgga gcgtacttaa gtacgtgagg
     5761 atttcgagca ctgcccggga ccaaaatggc aagtaaaata gcctgatggg ataggctcta
     5821 agtccaacga accagtgtca ccactgacac aatgaggaaa accatgtcac atctcgcaga
     5881 actggttgcc agtgcgaagg cggccattag ccaggcgtca gatgttgccg cgttagacaa
     5941 tgtgcgcgtc gaatatttgg gtaaaaaagg gcacttaacc cttcagatga cgaccctgcg
     6001 tgagctgccg ccagaagagc gtccggcagc tggtgcggtt atcaacgaag cgaaagagca
     6061 ggttcagcag gcgctgaatg cgcgtaaagc ggaactggaa agcgctgcac tgaatgcgcg
     6121 tctggcggcg gaaacgattg atgtctctct gccaggtcgt cgcattgaaa acggcggtct
     6181 gcatccggtt acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc ttggctttac
     6241 cgtggcaacc gggccggaaa tcgaagacga ttatcataac ttcgatgctc tgaacattcc
     6301 tggtcaccac ccggcgcgcg ctgaccacga cactttctgg tttgacgcta cccgcctgct
     6361 gcgtacccag acctctggcg tacagatccg caccatgaaa gcccagcagc caccgattcg
     6421 tatcatcgcg cctggccgtg tttatcgtaa cgactacgac cagactcaca cgccgatgtt
     6481 ccatcagatg gaaggtctga ttgttgatac caacatcagc tttaccaacc tgaaaggcac
     6541 gctgcacgac ttcctgcgta acttctttga ggaagatttg cagattcgct tccgtccttc
     6601 ctacttcccg tttaccgaac cttctgcaga agtggatgtc atgggtaaaa acggtaaatg
     6661 gctggaagta ctgggctgcg ggatggtgca tccgaacgtg ctgcgtaacg ttggcatcga
     6721 cccggaagtt tactctggtt tcgccttcgg gatggggatg gagcgtctga ctatgttgcg
     6781 ttacggcgtc accgacctgc gttcattctt cgaaaacgat ctgcgtttcc tcaaacagtt
     6841 taaataaggc aggaatagat tatgaaattc agtgaactgt ggttacgcga atgggtgaac
     6901 ccggcgattg atagcgatgc gctggcgaat caaatcacta tggcgggcct ggaagttgac
     6961 ggtgtagaac cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt ggttgagtgt
     7021 gcgcagcatc cgaacgctga caaactgcgt gtgacaaaag tgaatgtcgg cggcgatcgc
     7081 ctgctggaca tcgtctgcgg tgcgccaaac tgccgtcagg gcctgcgtgt ggcggtagcg
     7141 accattggtg ctgttctgcc gggtgatttc aaaattaaag cggcgaaact gcgcggcgaa
     7201 ccgtctgaag ggatgctgtg ctccttttct gagctgggaa tttctgacga ccataacggc
     7261 attatcgaac tgcctgcaga tgcgccgatt ggcactgaca tccgcgaata cctgaaactc
     7321 gatgacaaca ccatcgaaat cagcgtaacg ccaaaccgtg cagactgttt aggtatcatt
     7381 ggtgttgcgc gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc ggaaatcgtt
     7441 ccggttggtg cgaccatcga cgacacgctg ccgattacag tcgaagcgcc ggaagcctgc
     7501 ccgcgttatc ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac tccgctgtgg
     7561 atgaaagaaa aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt tgacgtcacc
     7621 aactatgtgc tgctcgaact gggccagccg atgcacgctt tcgataaaga tcgcattgaa
     7681 ggcggcattg tggtgcggat ggcgaaagag ggtgaaacgc tggtgctgct tgatggcact
     7741 gaagcgaagc tgaatgctga caccctggtt atcgccgacc acaacaaggc gctggcgatg
     7801 ggcggcattt ttggtggcga acactctggc gtgaatgacg aaacgcaaaa cgtcctgctg
     7861 gaatgcgctt tcttcagccc gctgtctatc accggtcgtg ctcgtcgtca tggcctgcat
     7921 actgatgcgt ctcaccgtta tgagcgtggc gttgatccgg cactgcagta caaagcgatg
     7981 gaacgtgcga cccgtctgct gatcgacatc tgcggtggtg aggctggtcc ggtaattgat
     8041 atcaccaacg aagcaacgct gccgaagcgt gcaaccatca ctttacgtcg tagcaaactg
     8101 gatcgcctga tcggccatca tattgcggat gagcaggtaa ctgacattct gcgtcgtctc
     8161 ggctgcgaag tgaccgaagg caaagacgag tggcaggcag ttgcgccgag ctggcgtttc
     8221 gacatggaga ttgaagaaga tctggtcgaa gaagtcgcgc gtgtttacgg ctacaacaac
     8281 atcccggatg agccggtaca ggcaagcctg attatgggta ctcaccgtga agctgacctg
     8341 tcgctcaagc gcgttaaaac gctgctcaac gacaaaggct atcaggaagt gatcacctac
     8401 agcttcgttg atccgaaagt gcagcagatg atccatccag gcgttgaagc cttactgctg
     8461 ccaagcccga tctctgttga aatgtcagca atgcgtcttt ctctgtggac cggcctgctg
     8521 gcaaccgttg tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt cgaaagcggt
     8581 ctgcgtttcg taccagatac tcaggcaccg ttgggcattc gtcaggatct gatgttagcc
     8641 ggtgtgattt gcggtaaccg ttacgaagag cactggaacc tggcaaaaga gaccgttgat
     8701 ttctatgatt tgaaaggcga tcttgaatcc gttctcgacc tgaccggtaa actgaatgag
     8761 gttgagttcc gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc agcgatttat
     8821 ctgaaaggtg aacgtattgg ttttgttggg gttgttcatc ctgaactgga acgtaaactg
     8881 gatcttaacg gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc agaccgcgtg
     8941 gtgcctcagg cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat cgcggtggtg
     9001 gtcgcagaaa acgttcccgc agcggatatt ttatccgaat gtaagaaagt tggcgtaaat
     9061 caggtagttg gcgtaaactt atttgacgtg taccgcggta agggtgttgc ggaggggtat
     9121 aagagcctcg ccataagcct gatcctgcaa gataccagcc gtacactcga agaagaggag
     9181 attgccgcta ccgtcgccaa atgtgtagag gcattaaaag agcgattcca ggcatcattg
     9241 agggattgaa cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt ttgataagct
     9301 tgggcttagc aagcgggatg ccaaagaact ggttgaactg tttttcgaag agatccgtcg
     9361 cgctctggaa aacggcgaac aggtgaaact ctctggtttt ggtaacttcg atctgcgtga
     9421 taagaatcaa cgcccgggac gtaacccgaa aacgggcgag gatattccca ttacagcacg
     9481 gcgcgtggtg accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa acgcttcgcc
     9541 caaagacgag taatctgatc taactaaaaa ggccgctctg cggccttttt tcttttcact
     9601 gtcgaagagt caccgtaaaa tcaacgccat gacacttcag cagaacggat accatgctga
     9661 cacttgcccg ccaacaacag cgacaaaata ttcgctggtt attatgcctg tcagttttga
     9721 tgctgctggc gcttctctta agcctttgcg ccggtgaaca atggatctcg ccaggtgact
     9781 ggttttctcc tcgtggcgaa ctgttcgtct ggcaaattcg cctgccacgt acgctggctg
     9841 tattgctggt tggtgcggcg ctggctatat ccggcgctgt aatgcaggcg ttgtttgaaa
     9901 atcctctggc agaacctgga ctacttggcg tctctaacgg cgcaggcgtg gggcttatcg
     9961 ccgcggtatt gcttgggcaa gggcaactcc ccaactgggc gctagggctg tgtgcgattg
    10021 ctggcgcgct tatcatcact ttaatactct tacgtttcgc ccgtcgtcat ctttcgacca
    10081 gtcggttatt gctggctggc gttgcattag ggattatctg tagcgcacta atgacgtggg
    10141 ctatctactt ttccacctct gttgatttac gtcagctgat gtactggatg atgggcggtt
    10201 ttggcggcgt agactggcgg caaagctggc tgatgctggc attgatcccc gtgttgttgt
    10261 ggatctgttg tcagtccagg ccgatgaata tgttagcact tggcgagatc tcggcgcggc
    10321 aactgggttt acccctgtgg ttctggcgca atgtgctggt ggcagcgacc ggctggatgg
    10381 ttggcgtcag tgtagcgctg gcgggtgcta tcggctttat tggtctggtg atcccacata
    10441 ttcttcggtt gtgtggttta accgatcatc gcgcattact tcccggctgc gcgctggcag
    10501 gggcgagcgc attgctgctg gccgatattg tagcgcgcct ggcattagct gccgcagagc
    10561 tgcctattgg cgtggtcacc gcaacgttgg gtgcgccggt gtttatctgg ttattgttaa
    10621 aagcaggacg ttagccgcaa aaagacggtc tatgattaaa agctaagatt tttactgacc
    10681 acacccagga gaaacgatgc aagattccat tctgacgacc gtagtgaaag atatcgacgg
    10741 tgaagtgacc acgctggaga agtacgccgg taatgtgctg ttgattgtca atgtcgcctc
    10801 aaagtgtggc ttaacgccgc aatatgagca gttggagaat attcagaaag cctgggccga
    10861 tcgaggtttt gtggtgctgg gattcccgtg caaccagttt ctggaacaag aaccgggcag
    10921 cgatgaagag attaaaactt actgtaccac cacatggggt gtgacgttcc cgatgttcag
    10981 taagattgaa gttaacggcg aaggacgcca tccgctgtat caaaaattga tttccgcagc
    11041 gccgaccgca gtcgcgccgg aagagagcgg attctatgcc cgtatggtca gcaaaggtcg
    11101 tgcaccgctg taccctgatg atattttatg gaattttgag aaatttttag ttggcaggga
    11161 cggattggtc atccagcgtt tttccccgga tatgacgccg gaagatccca ttgtgatgga
    11221 aagcattaaa ctggcgttgg caaaataatg tctattgtga tgcagttaca ggatgttgcg
    11281 gaatctaccc gcctggggcc gctttctggc gaggttcggg ctggggagat cctgcacctg
    11341 gtggggccga atggcgcggg taagagtacc ttactggcgc gaatggccgg aatgaccagc
    11401 ggtaagggaa gcattcagtt cgcggggcaa ccactggaag catggtccgc aacaaaactc
    11461 gcgctgcatc gtgcctatct ttcacaacag cagacgccgc cgtttgcaat gccggtctgg
    11521 cactacctga cactgcatca gcacgataaa acgcgtaccg aactactgaa tgatgtcgca
    11581 ggggcgctgg ctcttgatga caaactcggg cgtagcacca atcaactttc cggcggtgaa
    11641 tggcaacgcg tacgtcttgc tgcggtggtg ctgcaaatca caccacaagc caatcccgca
    11701 ggccaattgc tgcttcttga tgagccgatg aacagtcttg atgttgcgca acaaagtgcg
    11761 ttagacaaaa ttctgagcgc gctgtgtcag caaggactgg cgattgtgat gagcagtcac
    11821 gatctcaacc acacattgcg tcatgcgcat cgggcgtggt tgctaaaagg tggaaaaatg
    11881 ctggccagtg gacgcagaga agaggtgctc acgccgccaa atctggcgca ggcctatggg
    11941 atgaattttc gccgtctgga tatcgaaggt cacagaatgc tgatttcgac catctgattt
    12001 cattagtatc ttgaaaatcg ggtaattacg acgctaaatt aatgcaagaa taaaaaacag
    12061 aggaaccgta cggaatgcgt ttctgcctta ttttgatcac agcactgttt ctggccgggt
    12121 gtagccacca taaagcaccg ccgccaaatg ccagactttc tgattcgatt accgttattg
    12181 ccggtttgaa cgaccagcta caaagctggc atggcacgcc gtatcgttat ggtggcatga
    12241 cgcggcgcgg tgtggactgt tcgggatttg tggttgtgac gatgcgcgat cgtttcgatt
    12301 tgcagctgcc ccgagaaacc aaagaacaag cctctatcgg cacgcaaatt gataaagacg
    12361 agttgctgcc tggtgacctg gtctttttca aaacgggctc cggacaaaat ggtttgcatg
    12421 taggtattta cgataccaac aaccaattta tccacgcctc taccagcaag ggagtgatgc
    12481 gttcctcact tgataatgtc tattggcaga aaaatttctg gcaggcgaga cgaatctagt
    12541 atgtcgaaaa ttggacggcg atgatacatc gccgtccatt tactccagaa tacagtagta
    12601 ttttgcagac aaaaattccg taaatggatt attcgtttat aatcaggata atctaaaaaa
    12661 aggacccctg aaaaagtggt taacatggaa caaatgaaag ttactccttc tgtaattgtt
    12721 atgtcttatg gcctcatccc ttttagccgg atactgaaaa acatccttcg agagggacgg
    12781 ttaccatgaa gatttttttg gagaatcttt atcattctga ttgctatttc ctgccgataa
    12841 gagataacca acaagtcctt gtcggcgttg agttaattac ccatttctcc agtgaagatg
    12901 gcacggtgcg tatcccgacg agtcgggtta tagcgcaact taccgaggag caacattggc
    12961 aattattttc agagcagttg gaattactaa aatcatgcca acattttttt attcaacata
    13021 aactctttgc ctggcttaat ttaacgccac aagtggctac attattatta gagagggata
    13081 attacgcagg cgaactatta aaatatccgt ttattgaact gttgattaat gagaattacc
    13141 cgcatctcaa tgaaggaaaa gataatcgcg gtcttttatc cttatcgcaa gtgtatcccc
    13201 ttgtgttagg taatttggga gcaggcaata gcacaatgaa agcggttttt gatggtcttt
    13261 tcacccgcgt catgctggat aaaagtttta ttcagcaaca aatcacgcat cgctccttcg
    13321 aaccgtttat tcgtgcgatt caggcgcaaa tttccccttg ttgcaattgc atcatcgcgg
    13381 ggggcattga tacgacagaa atattggcac agattacccc cttcgatttc catgctctcc
    13441 agggatgcct gtggcctgcc gttcctataa atcagataac gacgttggtt cagcgataac
    13501 cctcctgttt gctggtgttt aagacgagag taaccgtcta cactatcaaa caggaggatc
    13561 tatgaccctg tcttttatta cccgctggcg cgatgaattg ccagcaacct atacagcact
    13621 ttcccctacg cctttaaata atgcccggct gatttggcat aataccgaac tggctaacac
    13681 gctgagtatt ccatcgtcgc tgtttaaaaa tggcccaggt gtctggggcg gcgaaaccct
    13741 actgcctggt atgtcaccac tggcgcaggt ttacagtggt catcagttcg gcgtctgggc
    13801 gggccaactg ggtgatgggc gcggcatttt actcggcgaa caacagctgg ccgatggcac
    13861 tacaatggac tggcatctga aaggtgctgg cctgacgcct tattcgcgaa tgggtgatgg
    13921 acgggcggtt ttacgttcga cgatacgaga aagtctcgcc agtgaggcga tgcattatct
    13981 gggcattccg acgacccgcg cgttaagtat cgtcaccagc gattcgccag tgtatcggga
    14041 aacggtggag ccaggcgcga tgctgatgcg tgtggcacca agtcatctgc gctttggcca
    14101 tttcgaacat ttttactatt gccgcgagcc ggaaaaggtt cgtcagttgg ctgactttgc
    14161 cattcgtcat tactggtcac atcttgaaga tgatgaggac aaataccgtc tctggtttag
    14221 cgatgttgtc gcacgtaccg catcgctaat tgcccaatgg cagacggtcg gctttgctca
    14281 tggggtgatg aataccgaca acatgtcgct gctggggctg acgcttgatt acgggccgtt
    14341 tggttttctt gatgattacg aacccggttt tatttgtaat cactcggatc atcaagggcg
    14401 ttatagcttt gataatcaac ctgctgtcgc gttgtggaat ttacagcgtc tggcgcagac
    14461 attgtcacca tttgttgccg tagatgccct gaatgaggcc ctggacagct atcagcaggt
    14521 tttgttgacg cattatggac aacggatgcg gcagaaactg ggcttcatca cggagcaaaa
    14581 agaggataac gcgctactga atgaattatt cagtctgatg gcgcgagagc gcagcgatta
    14641 tacccgcaca tttcgcatgc tgagtctgac cgagcagcac agcgcggcgt caccgctacg
    14701 tgatgagttt attgatcgtg cggcatttga tgactggttt gcccgttatc gggggcgttt
    14761 gcaacaagac gaggttagcg atagtgagcg tcagcagttg atgcaaagcg ttaaccccgc
    14821 tctggtattg cgcaactggt tggcgcaacg ggcgattgaa gcggcagaaa agggtgatat
    14881 gacggaattg caccgcctgc atgaggcgtt gcgaaatcct ttcagcgaca gagatgatga
    14941 ctatgtcagt cgtccacctg actggggtaa acggctggaa gtcagctgct cgagttaaag
    15001 gctacttggt caacagcagc ttgccagcct gagttttacg gagcagatat tcttgcccgt
    15061 catgatcgat aatcagtttg ccatccggac ctaacagtgt ctggctggaa attcgccgaa
    15121 taggctgcgg ggaagcggtc ttgtgattgg catccgtttc aggcgtgagt tttctgctat
    15181 ccgtataacg cat
//
LOCUS       DAZFFG010000107        15181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFFG010000107 DAZFFG010000000
VERSION     DAZFFG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.107"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            103..192
                     /gene="kdpF"
                     /locus_tag="ACHJ7J_003806"
     CDS             103..192
                     /gene="kdpF"
                     /locus_tag="ACHJ7J_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO5001983.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            192..1865
                     /gene="kdpA"
                     /locus_tag="ACHJ7J_003807"
     CDS             192..1865
                     /gene="kdpA"
                     /locus_tag="ACHJ7J_003807"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO5001984.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1888..3936
                     /gene="kdpB"
                     /locus_tag="ACHJ7J_003808"
     CDS             1888..3936
                     /gene="kdpB"
                     /locus_tag="ACHJ7J_003808"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO5001985.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAVADKVPADQLRKGDTVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLSANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3945..4517
                     /gene="kdpC"
                     /locus_tag="ACHJ7J_003809"
     CDS             3945..4517
                     /gene="kdpC"
                     /locus_tag="ACHJ7J_003809"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO5001986.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQDSGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLENNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4510..7194
                     /gene="kdpD"
                     /locus_tag="ACHJ7J_003810"
     CDS             4510..7194
                     /gene="kdpD"
                     /locus_tag="ACHJ7J_003810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO5001987.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPVRTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7191..7868
                     /gene="kdpE"
                     /locus_tag="ACHJ7J_003811"
     CDS             7191..7868
                     /gene="kdpE"
                     /locus_tag="ACHJ7J_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO5001988.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
     gene            complement(8043..9683)
                     /gene="pgm"
                     /locus_tag="ACHJ7J_003812"
     CDS             complement(8043..9683)
                     /gene="pgm"
                     /locus_tag="ACHJ7J_003812"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO5001989.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(9709..10254)
                     /gene="seqA"
                     /locus_tag="ACHJ7J_003813"
     CDS             complement(9709..10254)
                     /gene="seqA"
                     /locus_tag="ACHJ7J_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO5001990.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            10439..11203
                     /gene="ybfF"
                     /locus_tag="ACHJ7J_003814"
     CDS             10439..11203
                     /gene="ybfF"
                     /locus_tag="ACHJ7J_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5001991.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            11343..11636
                     /gene="ybfE"
                     /locus_tag="ACHJ7J_003815"
     CDS             11343..11636
                     /gene="ybfE"
                     /locus_tag="ACHJ7J_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO5001992.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            11776..12306
                     /gene="fldA"
                     /locus_tag="ACHJ7J_003816"
     CDS             11776..12306
                     /gene="fldA"
                     /locus_tag="ACHJ7J_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO5001993.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            12516..12602
                     /gene="uof"
                     /locus_tag="ACHJ7J_003817"
     CDS             12516..12602
                     /gene="uof"
                     /locus_tag="ACHJ7J_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO5001994.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            12595..13041
                     /gene="fur"
                     /locus_tag="ACHJ7J_003818"
     CDS             12595..13041
                     /gene="fur"
                     /locus_tag="ACHJ7J_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO5001995.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(13125..13451)
                     /gene="chiQ"
                     /locus_tag="ACHJ7J_003819"
     CDS             complement(13125..13451)
                     /gene="chiQ"
                     /locus_tag="ACHJ7J_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO5001996.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(13501..14907)
                     /gene="chiP"
                     /locus_tag="ACHJ7J_003820"
     CDS             complement(13501..14907)
                     /gene="chiP"
                     /locus_tag="ACHJ7J_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO5001997.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         3391 a         3932 c         4165 g         3693 t
ORIGIN      
        1 atttttatac tttttttaca ccccgtccgc agatttttgc gaaatctttg cagccagaat
       61 tctacccttc cggtatcact tttaggccac tggaggtgca ctgtgagtgc aggcgtgata
      121 accggcgtat tgctggtgtt tttattactg ggttatctgg tttatgccct gatcaatgcg
      181 gaggcgttct gatggctgcg caagggttct tactgatcgc cacgttttta ctggtgttaa
      241 tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac attcctcttc
      301 caggtacagc gggcgttgaa cgtatactct ttcgtttacc tggcgtctct gaccatgaga
      361 tgaactggaa gcagtatctc tgtgccattc tcggcctgaa catgctgggg ctggcggtgc
      421 tgttttttat gttgctcggt cagcactatc tgccgcttaa tccacagcag ttgccggggc
      481 tgtcgtggga tctggcgctg aataccgccg tcagctttgt cacgaatacc aactggcaat
      541 cttatagcgg tgaaaccacg ttgagctatt tcagccagat ggcgggctta acggtgcaaa
      601 actttctttc tgccgccagc gggattgcgg tgatttttgc cctcattcgt gcatttaccc
      661 gccagagcat gagcacactc gggaatgcct gggtcgatct gctacgcatc acgttatggg
      721 tgctcgtccc tgtggcgttg gtgatcgcac tgttttttat tcaacaaggt gcgctgcaaa
      781 actttctgcc ctatcaggca gtgaataccg ttgaaggagc gcaacagctg ttaccaatgg
      841 gtccggtggc ttctcaggaa gcgatcaaga tgctcggtac taacggcggt ggtttcttta
      901 atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc gtgcagatgc
      961 tggcgatctt cttgatccca acagcgctgt gctttgcctt tggtgaagtg acgggcgatc
     1021 gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc tgcgtaggcg
     1081 tggtgatgtg ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc gcagacagca
     1141 gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg
     1201 tcgtgactac ggcggcgtcc tgtggcgcgg tgattgcgat gcatgattcg tttaccgctc
     1261 tcggtggcat ggtgccgatg tggctgatgc aaattggtga agtggtattc ggcggtgtcg
     1321 gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt gccgggctga
     1381 tgattggtcg tacaccggaa tatctgggta aaaaaatcga cgtacgtgag atgaaactga
     1441 ctgcactggc gattctggtc accccgacgc tggtgctgat gggcgcggcg ctggcaatga
     1501 tgaccgacgc cggacgtagc gccatgctca accctggccc gcatggtttt agcgaagtgc
     1561 tgtacgccgt gtcatccgcc gctaacaaca acggcagcgc ctttgccgga ttaagcgcca
     1621 actctccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc ttcggggtga
     1681 ttatcccggt gatggcaatt gccggttcgc tggtgagtaa aaagagccaa cccgccagct
     1741 ccggcacgct gccaacgcac ggcccgctgt ttgttggcct gttaatcggc accgtgttgc
     1801 tggttggcgc actgaccttt atccctgccc tggcgcttgg tccggtggcg gaatatctct
     1861 cctgatgata ttgagtgagc actgaatatg agtcgtaaac aactggcgct attcgaacca
     1921 acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca ggcgcaatgg
     1981 cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc tgaccacctg tattagcatc
     2041 gcgatggcaa gcggcgcgat gcctggcaat gcgctgttta gcgcggcaat tagcggctgg
     2101 ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg ccgcagtaaa
     2161 gcgcaggcca acagtctgaa aggggtgaaa aaaactgctt ttgctcgcaa gctgcgcgaa
     2221 ccgaaatatg gcgctgtggc agacaaagtt cctgccgatc aactgcgtaa aggtgacacc
     2281 gtgctggtag aagctggcga tattatcccc tgcgatggtg aagtcattga aggcggcgca
     2341 tcggtcgatg aaagcgccat caccggggaa tcggcaccgg tgatccgtga atccggcggc
     2401 gattttgcct ccgtcactgg cggcacgcgt attctttctg actggctggt gattgagtgt
     2461 agcgttaacc ccggcgagac atttctggat cggatgatcg cgatggtgga aggcgcacag
     2521 cgacgtaaaa cgccgaacga gattgccctg accatcctgc tgattgccct gactatcgtc
     2581 tttttactgg caaccgccac gctgtggccg ttttccgcgt ggggcggtaa tgcagtcagc
     2641 gtaacggtac tggtggcgct gttggtctgt ctgatcccaa ccactattgg cggcctgttg
     2701 tcagcgatcg gcgtcgccgg gatgagtcgg atgctgagcg cgaatgtgat tgccaccagc
     2761 gggcgggcgg ttgaagcggc aggtgacgtt gacgttctgc tgctggataa aaccggcacc
     2821 atcacactcg gtaaccgtca ggcatcggag tttatccccg cgcaaggcgt ggatgaaaaa
     2881 acgctggctg acgccgcaca actggcttcg ctggctgatg aaacgccgga aggccgcagt
     2941 attgtgatcc tcgccaagca acgttttaac ctgcgcgaac gcgatgtgca gtcgctccat
     3001 gccacctttg taccgtttac tgcgcaaagc cggatgagcg ggatcaacat cgacaaccgc
     3061 atgatccgta aaggttctgt cgatgccatt cgccgccatg ttgaggctaa cggtggtcac
     3121 ttccctgccg atgtcgatca aaaagtcgat caggttgcgc gtcagggggc cacgccgctg
     3181 gtggtggtgg aaggttcccg tgtgctgggc gttattgcgc tgaaagatat cgtcaaaggc
     3241 ggtattaaag agcgcttcgc ccagctgcgt aaaatgggca ttaaaacggt gatgattacc
     3301 ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggcgtcga tgattttctc
     3361 gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc ggaaggccgt
     3421 ttggtggcga tgaccggcga cggcaccaat gatgctccgg cgctggcgca ggcagatgtt
     3481 gcggtggcga tgaactccgg cactcaggcg gcgaaagaag cgggcaatat ggtggatctc
     3541 gactctaacc cgaccaagtt gatcgaggtg gtacatattg gcaaacagat gctgatgacc
     3601 cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt cgccattatt
     3661 ccggcggcat tcgcggtaac gtatccacag ttaaatgcgc tgaacatcat gcgcctgcat
     3721 tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat cgtctttttg
     3781 attcccctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc catgttgcgc
     3841 cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg tatcaaagtc
     3901 attgatttac tactgaccgt ttgcggtctg gtgtgaggtt taccatgcgt ggattacgtc
     3961 cggcattatc gacatttatc tttctgttat tgattactgg cggtgtttac ccgctgctga
     4021 ccaccgcgct ggggcaatgg tggtttccct ggcaggccaa cggttcgttg atccgtgaag
     4081 gtgatacggt gcggggttcg gcattaatcg ggcagaattt taccgacaac ggctattttc
     4141 atggtcgccc gtcggcaacg gcagaaatgc cttataatcc acaggattct ggcgggagca
     4201 atctggcggt cagtaaccct gagctggata aactaatagc cgcacgcgtt gccgcattac
     4261 gggccgctaa cccggatgcc agcgcgagcg ttccggttga actggtgacg gcatcggcaa
     4321 gcgggctgga aaataacatc accccacaag cggcggcctg gcagatccca cgcgtagcga
     4381 aagcgcgtaa tctcagcgtt gaacagctca cgcaactgat cgcaaaatac agccaacaac
     4441 cactggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat ctggcgctgg
     4501 ataaacttga tgaataacga acccttacgt cccgaccccg atcgtctgct ggaacaaact
     4561 gccgcgccgc atcgggggaa gctgaaagtt ttcttcggtg cctgcgcagg cgtcgggaag
     4621 acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga tattgtggtt
     4681 ggcgtggtag aaacccacgg gcggaaagat accgccgcca tgctggaagg gctggctgtt
     4741 ctgccgccaa aacgcctggc gtaccgtggg cggcacatca gcgagtttga tctcgatgcc
     4801 gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag taatgcgcca
     4861 ggttcccgtc atcccaaacg ctggcaggat atcgaagaac tgctggaagc tggcattgat
     4921 gttttcacta ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt cagcggcgta
     4981 accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc cgacgacgtg
     5041 gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg caaagtctat
     5101 attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa tctgatcgcc
     5161 ctgcgcgaac tggcactgcg ccgtacagcc gatcgcgttg atgagcaaat gcgcgcctgg
     5221 cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct tttatgcatc
     5281 ggccataaca ccggcagcga aaaactggtc cgcgcggcgg cgcggctggc gtcacggctg
     5341 gggagcgtct ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt accggagaaa
     5401 aaacgtcggg caattctcag cgccttacgt ctggcgcagg agctgggcgc ggagacggca
     5461 acactttctg atccagcgga agagaaagcg gtagtgcgtt atgcccgtga acataatctc
     5521 ggcaagatta ttctcggtcg cccagcctcg cgccgctggt ggcgtcggga aacgtttgct
     5581 gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct tgatgaacca
     5641 cccgtccgca cgattaacaa cgcgccggat agccgctctt ttaaagacaa gtggcgggta
     5701 cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt aattgccatg
     5761 cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctact tggcgtggtg
     5821 gtggtggcgc tattttatgg acgctggcct tcggtggttg ctaccgtcat taatgtggtg
     5881 agtttcgatc tcttttttat cgccccacgc ggcacgctcg ccgtctctga tgtgcaatat
     5941 ctgctgacct tcgcggtgat gttaaccgtc gggctggtga tcgggaacct tactgcgggt
     6001 gtgcgttatc aggcacgggt agcccgttac cgcgagcaac gcacacggca cttatatgaa
     6061 atgtcgaaag ctctggcggt gggccgcagt ccgcaggaca tcgccgccac cagcgaacaa
     6121 tttattgcct ccacgtttca tgcccgcagt caggtgttgt tgcccgatga caacggtaaa
     6181 ttgcagccgt taacacatcc gcaaggaatg acgccgtggg acgatgccat cgcgcagtgg
     6241 agttttgata aaggcctgcc tgcgggtgcg ggcaccgaca cgttacccgg cgtgccgtac
     6301 cagattttgc cgttaaaaag cggtgagaaa acctacgggt tggtggtggt ggaaccgggg
     6361 aatctgcgcc agttgatgat cccggaacag cagcgcctgc tggagacgtt tacgctgtta
     6421 gtcgccaatg cgcttgagcg gctgacgctg accgccagcg aagaacaggc gcggatggca
     6481 agcgaacgtg aacagatccg caacgccctg ctggcggcgc tttcgcatga tttacgcact
     6541 ccgctcacgg tgttgttcgg tcaggcagaa atcttaacgc tcgatctggc aagcgaagga
     6601 tcaccccacg cccgccaggc cagcgagatt cgtcagcatg tgctgaacac tacccgactg
     6661 gtgaataatc tactggatat ggcgcgaatt cagtccggcg gctttaattt gaagaaagag
     6721 tggttaacgc tggaagaagt agtcggcagc gcgctgcaaa tgctggaacc gggtttatcg
     6781 tcgcccatca atctttctct gccagaaccg ctgaccttaa tccacgttga cgggccactc
     6841 tttgaacggg tgctgattaa tttgctggag aacgcggtga aatatgcggg tgcgcaggcc
     6901 gaaattggta tcgatgccca cgttgagggc gaaaatctac aactggatgt ctgggataac
     6961 ggccccggtc ttccgccagg ccaggagcag acgatatttg ataagtttgc tcgcgggaat
     7021 aaagagtcgg cagtaccggg ggtagggctt ggactggcaa tttgtcgggc gatagtggat
     7081 gtacacgggg gcactattac cgcgttcaac cgaccggaag gtggtgcctg ttttcgtgtt
     7141 acacttcccc agcaaactgc ccctgaactt gaagaatttc atgaggatat gtgacaaacg
     7201 ttctgattgt tgaagatgaa caggctattc gtcgctttct gcgcacggcg ctggagggcg
     7261 acgggatgcg cgtctatgag gccgaaacgc tgcaacgcgg cttgctggag gcggctaccc
     7321 gtaagccaga tttgattatt ctcgatcttg gcctgcccga tggtgatggg attgagttta
     7381 tccgcgacct gcgccagtgg agcgcggtgc cggtgattgt gctttccgca cgcagcgaag
     7441 agagcgacaa aatcgccgcg ctggatgccg gagcggatga ttatctgagt aagccgtttg
     7501 gcattggcga attgcaggcc cgtctgcgcg tcgctttacg ccgccactcc gccaccgccg
     7561 cgcccgatcc gctggtgaaa ttttccgatg ttaccgtcga tttagccgcc cgcgtgattc
     7621 accggggtga ggaagaggtg catctcaccc cgattgagtt tcgcctgctg gcagtgctgc
     7681 tcaacaacgc cggaaaagtg ctcacccagc gccaactcct taaccaggtg tgggggccaa
     7741 acgcggtcga acacagtcat tatttgcgta tttatatggg acatctgcga caaaaactgg
     7801 aacaggatcc cgctcgcccc agccatttca ttactgaaac cggtattggt tatcggttta
     7861 tgctttaggc ggttaaatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcgc
     7921 gtaaattcaa tatattgcac catcctcgta ggcctgataa gcgtagcgca tctggcaatt
     7981 ctgtgtttgt catacgtaaa aaagggcgat cttccgaccg ccctttttct tatttaatgt
     8041 gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc gatctgcttg
     8101 cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc cgtgcctgac
     8161 ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccaat agaagcaccg
     8221 ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac cagccagggt gctggcgctc
     8281 accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc agctgcctgc
     8341 aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg ttcctgcggg
     8401 ttcttaccgg tgacagcggt gatttccgcc gccagcagac acatgatgat gccgtctttg
     8461 tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct ctcttcgccg
     8521 ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc taccgggact
     8581 tctaccagtt tacggcccaa gtcgttgacc acacggtcga tcatcgcaga agaaaccagc
     8641 gttttaccaa cggcaacatc tttgccccac tgcggacgat gctggaacag gtaattgatt
     8701 gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat accgtgacgg
     8761 tcataatccg ggtcgttagc aaaagccaga tcgaacttat cacgcagtgc cagcaggcct
     8821 gccattgcac actcagagga gcagtccata cggatcgcgc cgtctttatc aaggtgcata
     8881 aagcggaagg tttgatcgac ctgatcgtta acgatagtca ggttgaggtt gtaatactcg
     8941 ccaatacgct tccagtattc gataccggaa ccgcccaacg gatcaacgcc aagcgtcagg
     9001 cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac aaacggctgc
     9061 accagatcct gctctttcac atgaccggat gccatcgctt cgtcgaggga gatacgcttc
     9121 acgcctttca ggccatcggc catcagtgcg ttggctctgt cttccaccac tttagtgacg
     9181 ttggtatctg ccgggccacc atttggcgga ttgtatttga ttccaccatc ttccggcggg
     9241 ttatgggacg gtgtaatcac gataccgtct gccagcgggc cacctttttt attgtgaacc
     9301 aggatggcat tggaaacggc aggcgtcggg gtgaagccat tgttttcctg cacaataaca
     9361 tctacgccgt tcgctgccag cacttccaga acggaaataa atgcaggttc ggacagggcg
     9421 tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg ttcttcagca
     9481 attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc tgcactgcca
     9541 cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc tggtttcagt
     9601 acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg tgcaggttgg
     9661 cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt agatagttcc
     9721 gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt gttcgatcat
     9781 gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc ctggcacatg
     9841 tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg caaagtaaac
     9901 gcgcgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt caagagaata
     9961 tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg cgtattcatc
    10021 cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga ccggcttcgc
    10081 ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag caggctgtga
    10141 tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc cgatatgctt
    10201 agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt tcatcttaat
    10261 ccagtgcagt gtcttagctg aatacaagtc gccaggagta tatgaataaa tcccggcagc
    10321 ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg ttgcagccct
    10381 gtggcacaat ggtcaacata ataccctaac ccaacaacgc gaaaaaaaga actttgccat
    10441 gaaattgaat atccgcgcgc aaactgcaca aaaccagcac aataattctc ccatcgttct
    10501 tgtccacggt ctgtttggca gcctcgacaa ccttggcgta ctggctcgcg atctggtaaa
    10561 cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga gagatccggt
    10621 gatgaattac ccggcgatgg cccaggatct tgttgatacc ctggatgcac tgcagatcga
    10681 caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac ttactgcact
    10741 ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg actatcacgt
    10801 acgccgtcat gatgagattt tcgcggctat caacgcggtc agtgaatcgg acgcacaaac
    10861 tcgccagcaa gcagcggcaa taatgcgcca gcatcttaat gaagaagggg tgattcagtt
    10921 tctgctgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat tgtgggatca
    10981 gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg ccctgtttat
    11041 ccctggtggt aattctccgt atgttagcga gcagtaccgt gatgatttac tggcacaatt
    11101 tccacaggca cgggcgcatg tcattgcagg cgcgggtcac tgggtccatg ctgaaaaacc
    11161 ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact gattaaaaac
    11221 ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg ctgatgtatt
    11281 atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa gtcaccaaga
    11341 tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac gagcgtcgac
    11401 cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt aataaacgca
    11461 accaactaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag ctgaacgcgg
    11521 aagctgtcga ggcgctgaac gagctggcgg aatcgcgcaa tatgagccgt agcgaactga
    11581 tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc gtttaaattt
    11641 ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca agactgcctg
    11701 ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa taagtttcaa
    11761 gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac cggtaatacc
    11821 gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga tgtccatgac
    11881 attgcaaaaa gcagcaaaga agatctggaa gcttatgaca ttctgctgct gggcatccca
    11941 acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac tctcgaagag
    12001 attgatttca atggcaaact ggttgcgctg tttggttgtg gtgaccagga agattacgcc
    12061 gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg cggtgcaacc
    12121 atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg tctggcagat
    12181 gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact gaccgctgaa
    12241 cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga aattctcaat
    12301 gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa tgccaatcaa
    12361 aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc cggtgttcaa
    12421 gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata tttgccaggg
    12481 acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc tcaagagcaa
    12541 attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt ccgcatgact
    12601 gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg tttaaaaatc
    12661 ctggaagtcc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt atacaaacgt
    12721 ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact gaaccagttt
    12781 gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt atttgaactg
    12841 acacagcaac atcaccacga tcacctgatc tgcctcgact gcggcaaggt tatcgaattt
    12901 agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg cattcgcctg
    12961 actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg cgaagatgag
    13021 cacgcgcacg aaggcaaata agccagtctg aacgagaaaa gccaacctgc gggttggctt
    13081 ttttatgcaa gggaaataag aaaagcgaga gttacagctc tcacttattt gttattactg
    13141 cgaatttcct gccagacctt atcacaatcg gctttcaccg cctgatcatt tccggtttgc
    13201 gttgcacgca gacactgctg ataatccagt acgcggacac tttcctggtc ggcaaactgc
    13261 tgatgttgtt tctctttctt cagcacgttt aacacgctct ggcaggcttc aattttttcc
    13321 ggcgaacctt gtgcggtgtt gatacaggcg ctgtatgcct ctttcagacg gctgtcttcc
    13381 tgtggcgcgg ttgattgcgc acaagctacc agccccgatg ccatcatggc gatgagaatc
    13441 agttttttca tagcaaattc ctcaaacgtg cggcaggcac aaaacctgcc gcgtcgggca
    13501 tcagaagatg gtgaatggtg cgattaccat aaatttcacg tcacgctcat cctggaagat
    13561 gttgccgtaa ccaccgcccc agcttgggat gtcggagtgg ttgtcgtatt cggtgaagtg
    13621 cagtttgaac atcgtgcctt tggcgcgacc gtcctgaatg gtgtagaccg catccaggct
    13681 gtatgcagac tcttcaatag tccggttttt gtcgtagtac gcatccggat tgctctgcca
    13741 ggtcgcaggt ttagcatccc atgcgtaaac gtaggaagcg ccgatggcga agcctggaag
    13801 attccagttt ttcaggtcat acatcgcacc gaagaagacc gctttttcgc cgttggcgtt
    13861 gaagtcagaa cggttatccc accagatatc caggcgaccg ttcgaagaag cgtaggttgg
    13921 agtcatacgt tgcaggaagt atccctgctg accgtcagct ttaacccagg tgccttcgag
    13981 acgcaaatcc actacgtcag ccgcccggta accaaaggtc aacgcttgca gccaggcggt
    14041 gccgtcataa agatcgttga cgctgcgatc gtcaacttta tcgcgggtac cgtagaactg
    14101 gtagctggtg gttaacgggc taccggcgat atcaaatttg tagctggctt tggcaaaata
    14161 ttgatcgata tacccttctg cctgaccaaa tgccgcttcc agtacgaagt tatttttgaa
    14221 gtcgtatttc gccccaaggg agtgcagata atcaactttg gtggttttat cgttctgata
    14281 aaactcatcc atttccagat gccacggtgc tttgtattcg ttggtccaca tgtaggagaa
    14341 actcaacgca ccagcatcgc cgtaatcaaa attcgctccg gcttccgcac cctgataagt
    14401 acccggcata aagctccagt gaggcgctaa cagcgtctga ccggttggct gaatataacc
    14461 tgccctcgcc caaaccggac cgtatttaaa tttggccgca gctttataca ggcttatacc
    14521 gcttttgtcg ccggaccagt cttcgtcata ggctttatta ctttttgaaa acgcgatttc
    14581 gttcgggtga gagctgtcgc cgttttccgc catttcaatc gccgtaaacg cagcaatatc
    14641 aaggccgaac atatcagcag cataaccgga ctgaaaatcg aggttggcat tccaggtgga
    14701 gtgagaaagg ttggttttgt atttgtcgcc gtcggtaaca tctttacggt cgcgttcacg
    14761 ctgccagtaa tagataccac cggttaaggt tgaatcgtcg atgaatcctt cggcgcgagc
    14821 ctccggcatt gccataaagc ccgacattgc tgtaacaccg gcgatagcca gcgccagcgt
    14881 actacgtttg ccactaaacg tacgcatggg ttaatcctct ttgacgtata aattgctgca
    14941 ccaaaggtga acagcgaaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    15001 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    15061 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg
    15121 cggttgaaag gcttgcagaa gaataaaagc gtgaatttcc agcaagatga aagcggttac
    15181 a
//
LOCUS       DAZFFG010000108        15171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFFG010000108 DAZFFG010000000
VERSION     DAZFFG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.108"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..185)
                     /locus_tag="ACHJ7J_003821"
     CDS             complement(<1..185)
                     /locus_tag="ACHJ7J_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5001998.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLRNFNGVPGLNREN
                     FIYISNIFLNIKQRNEK"
     gene            complement(414..1334)
                     /gene="malM"
                     /locus_tag="ACHJ7J_003822"
     CDS             complement(414..1334)
                     /gene="malM"
                     /locus_tag="ACHJ7J_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO5001999.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVNGKG"
     gene            complement(1577..2917)
                     /gene="lamB"
                     /locus_tag="ACHJ7J_003823"
     CDS             complement(1577..2917)
                     /gene="lamB"
                     /locus_tag="ACHJ7J_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO5002000.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2989..4104)
                     /gene="malK"
                     /locus_tag="ACHJ7J_003824"
     CDS             complement(2989..4104)
                     /gene="malK"
                     /locus_tag="ACHJ7J_003824"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO5002001.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4469..5659
                     /gene="malE"
                     /locus_tag="ACHJ7J_003825"
     CDS             4469..5659
                     /gene="malE"
                     /locus_tag="ACHJ7J_003825"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO5002002.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5813..7357
                     /gene="malF"
                     /locus_tag="ACHJ7J_003826"
     CDS             5813..7357
                     /gene="malF"
                     /locus_tag="ACHJ7J_003826"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO5002003.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     KRAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7372..8262
                     /gene="malG"
                     /locus_tag="ACHJ7J_003827"
     CDS             7372..8262
                     /gene="malG"
                     /locus_tag="ACHJ7J_003827"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO5002004.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8634..10109
                     /gene="xylE"
                     /locus_tag="ACHJ7J_003828"
     CDS             8634..10109
                     /gene="xylE"
                     /locus_tag="ACHJ7J_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO5002005.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECVPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10153..10563)
                     /gene="psiE"
                     /locus_tag="ACHJ7J_003829"
     CDS             complement(10153..10563)
                     /gene="psiE"
                     /locus_tag="ACHJ7J_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO5002006.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10689..10832)
                     /locus_tag="ACHJ7J_003830"
     CDS             complement(10689..10832)
                     /locus_tag="ACHJ7J_003830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002007.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            10833..11111
                     /locus_tag="ACHJ7J_003831"
     CDS             10833..11111
                     /locus_tag="ACHJ7J_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002008.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11158..13254)
                     /locus_tag="ACHJ7J_003832"
     CDS             complement(11158..13254)
                     /locus_tag="ACHJ7J_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5002009.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDSARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVAGLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGVDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13254..13991)
                     /locus_tag="ACHJ7J_003833"
     CDS             complement(13254..13991)
                     /locus_tag="ACHJ7J_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5002010.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSAEDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13988..14626)
                     /locus_tag="ACHJ7J_003834"
     CDS             complement(13988..14626)
                     /locus_tag="ACHJ7J_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO5002011.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWTRTMGWTEYKQVRYATARSVFKWDGTDTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14740..14982)
                     /gene="yjbE"
                     /locus_tag="ACHJ7J_003835"
     CDS             complement(14740..14982)
                     /gene="yjbE"
                     /locus_tag="ACHJ7J_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO5002012.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT         3545 a         3971 c         3886 g         3769 t
ORIGIN      
        1 tttttttcgt tccgttgttt tatatttaaa aaaatattgc tgatataaat aaagttttct
       61 cgatttaacc ctggcacccc attaaaatta cgtagtaaat caaaatctac tatattattt
      121 ccttgcacca caccatgttt tatcaaagag ttattgccgt caaactttga gatatgacct
      181 aacataaaac ctctgcatta cagaaatgaa ataactggaa aaagtagcct gagcgtccag
      241 acactaccgg cgaaaggaaa agaatctctg ataatgcatt gagataatgg atattcttag
      301 tgaagaagaa gtgaagtact ataaaaaatc gctcatatcc ctggaatgaa gcataaaaaa
      361 aggtgcgcca ggagacgcac cagttgttgc aaaatcagca ctgtggggcg taattacccc
      421 ttgccattta cactgctgat aaaggtggaa cgggcagatg tcgatcccag gcgttcagct
      481 tcatcaagca gttttaacgc cttatcaaca tcacctttcg cgacagcgtt tttgatcgcg
      541 gtattaaaat aactttccgt gtcgttgagc atcggctcgc ttttcttcac cggcaccgga
      601 gcaggtgcag ccacagctgg tgccgccgtg ttgcctaccg taaccggagc tggagcggaa
      661 gaaccaaata gtggtcctac caacacgctg gagctggagt tcgttttgac tttcagtttc
      721 agtaagccat cggtggtatg acgagcaacc ggatcgggaa tatccgggat cgagttacca
      781 acgcctttgg cataggcttt agccgggtcg agcagttggg tcgtctgctg gagatctttt
      841 tccgtggtaa agaccagaac ataaagtttt tgctgcccca acgccggtgt caggcgcata
      901 acgccttcca gccgatctgc actcatcacg cctggttcct ggtaggtgaa ataactgctg
      961 gggaagaagg ctgatggggt catgttctga tcaagaatca gcacgttcgg cgcaaataca
     1021 ctagtttgtt tgttcacttc gctggtcagc gtcagggtca gttcgccaat gtttgccggg
     1081 acgctgtacg cagcaaccgg accactgatg ccgggaacgt tcagttgttg gccgccggtc
     1141 gccagttggg tggtctgggt tttagattga tcgaccggtg tccaggtgag ttgttgcagc
     1201 gcggcagatg gaatggctgg cgcgtcgctg gtgttttgcg gtacgtagtt aacatcggca
     1261 aggctgattc caggcgcgct tgccagcaac cctgctgata aacagagggc gatgagactt
     1321 ttattcattt tcattgtttt cacctcaaaa tctggagctc agcggtagcc aggcaatagc
     1381 gcgctaaacc cgataatcag aggggctttc gcccctttca gataatgaca acctgttttt
     1441 atgccggatg cggcgtaaac gccttatccg gcctacattt gacagccgtt gtaggcctga
     1501 taagacgcgc aagcgtcgca tcaggcgttg gatgccggat gcggcgcaag caccttatcc
     1561 ggcccaggtt ttgctattac caccagattt ccatctgggc accgaaggtc cactcgtcgc
     1621 tgtcgccacg accgaagctg ccgccgttga aatcagcagg aacggctttg ccgaagttag
     1681 tgttggtatt ggcgttaccg gtgtagtcgt aaccccattt ctcatcccac ttggcgtagg
     1741 ttgcgaagac acgaatagcc gggcgtgacc agatgctgtc gccagcctgc cattgttgtg
     1801 cgagggtaat tttgtactga ttgtttttgt cgccggtgcg ctgggattcg acgttgtcgt
     1861 agccgatttc catcacggtg ctcatgattg gcgtccactt gtacatcggg cgaataccga
     1921 cggtccacca cttggtgccg ttgtcgttat cccagttgat atcctggtac atacccacgt
     1981 acatcatgtc ccagttgtcg cccatggaga tcgcaccgtg gtcgaggata cgcagcatgt
     2041 gaccgttgtt gttgatattg taggcaaatt tttcgttatc aaatgcaacg ccagaaccct
     2101 gcgacagacc tttaccctgc gaggtcatcg agtcagtagc gtactgaaca acaaacttgt
     2161 taaagccctt caggacactc tgagtatgtt cagcagtgaa taaccagccg tctttcgatg
     2221 cgccatcaac cagacgatag ttatcacgca ggttggcacg accgtagtcg acacccagtt
     2281 ctaatgtgcc gcccgggttg atttccatct gcgctaaacg cacgtcgaaa acgtcgttcg
     2341 cggtttcgtt ggtatagtca taaatattgt tgctggcgaa agaggaagaa ccaccagctt
     2401 cagaggagcg ggttgctgcc agagagagtt taccgaagcc aacatcgatg ttttccagac
     2461 cggcaccagg accagaaata tcccagtagt agaagtcgat catatgaacg tcatgacgtt
     2521 ggtagaagcg cttacctgcc cagatggtgg agcctggcag ccattcgatc aggttcttac
     2581 cctgcacgtt tgcttcacgg aaggccggat cggtagcttc ccagtcattc tgttgcgcga
     2641 cggaataggc cacgttagtg tcgaaataga agctcttatc gccctctttc cacacttcct
     2701 gacccaattt taattcagca taagtttcac attcgttgcc aagacggtat ttactttgag
     2761 caccggtagt ctggaaacac tgttgttcac cgccgctacc tgtccagcca ataccggaac
     2821 gtgcatagcc gtggaaatca acagccattg cctgagcaga cattacgccc gctgcgacgg
     2881 caaccgccag aggaagtttg cgcagagtaa tcatcattct atctcctgag tcattgcttt
     2941 tcttttttca catcacctgt gacaggcttt gtgtgttttg tggggtgctt aaacgcccgg
     3001 ctccttatgc agtcgacgac atgcagtgcc atcctcacgg aacagatggc aacgctctgg
     3061 cggcaggccg atagcgaatg tggcaccttc ttctaccaac accacgtcgt tctggcggta
     3121 caccaggttt tgacgaatgg aagggatctg gatatggatt tgggtttcgt tgccgagttg
     3181 ctcgacgacc tgaacttcac cctcaaggat gacgtcagcg atatcactcg gcagtagatg
     3241 ttccgggcga atacccagcg acatattggc tccaacctgg acatcacggc tttcaacagg
     3301 cagccagact tgctgacgat ttggcatcgg cagctccacc tgcacttgat cgattgcagt
     3361 ggcggtcact tttaccggca ggaagttcat ctttggcgaa ccgataaatc cggcgacaaa
     3421 acggtctgcc ggatagtggt acagctccag cggtttccca acctgcgcca cgcgaccggc
     3481 gtccagcacc acgattttgt cggccagcgt catcgcttcg acctgatcgt gggtgacgta
     3541 aatcattgtg cggcccaggc gtttatgcag acgggagatt tcgatacgca tttgcacacg
     3601 cagtgcagca tcgaggttgg agagcggttc atcgagcaaa aatacgcttg gctcggccac
     3661 cagcgtacgg ccaatcgcca cacgctgacg ctgaccaccg gagagcgctt tcggtttgcg
     3721 atccagcaaa tgcgccagtt gtagcacttc cgccacctgg ttgacgcgtt ggttaatcac
     3781 ctcttttttt gcgcctgcca gtttcaggcc aaatgacatg ttttctgcta ctgacaggtg
     3841 gggatagagc gcgtaagact gaaacaccat accaacgccg cgttctgctg gcggagtgtc
     3901 attcatccgt ttctcaccga tgaacaggtc gccgctggtg atcgtctcaa gcccggcaat
     3961 catgcgcagt aaagtcgatt taccgcagcc agacggtccg acaaacacca cgaattcacc
     4021 ttcatggata tcgagattga tatctttcga taccacgacc tcgccccagg ctttcgttac
     4081 attttgcagc tgtacgctcg ccatgccctt ctccctttgt aacaacctgt catcgacagc
     4141 aacattcatg atgggctgac tatgcgtcat caggagatgg cttaaatcct ccaccccctg
     4201 gcttttttat gggggaggag gcgggaggat gagaacgcgg cttctgtgaa ctaaaccgag
     4261 gtcatgtaag gaatttcgtg atgttgcttg caaaaatcgt ggcgatttta tgtgcgcatc
     4321 tccacattac tgccaattct gtaacagaga tcacacaaag caacggtggg gcgtaggggc
     4381 aaggaggatg gaaagaggtt gccgtataaa gaaactagag tccgtttagg tgttttcacg
     4441 agcacttcac caacaaggac catagattat gaaaataaaa acaggtgcac gcatcctcgc
     4501 attatccgca ttaacgacga tgatgttttc cgcctcggct ctcgccaaaa tcgaagaagg
     4561 taaactggta atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa
     4621 gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata aactggaaga
     4681 gaaattccct caggttgcgg caactggcga tggccctgac attatcttct gggcgcacga
     4741 ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt
     4801 ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaatggca agctgattgc
     4861 ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc tgccgaaccc
     4921 gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggcaagag
     4981 cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg
     5041 gggttatgcg ttcaagtatg aaaatggcaa atacgacatt aaagacgtgg gcgtggataa
     5101 cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa
     5161 tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac
     5221 catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac
     5281 ggtactgccg accttcaagg gtcaaccatc taaaccgttc gttggcgtgc tgagcgccgg
     5341 tattaacgcc gccagtccga acaaagagct ggcgaaagag ttcctcgaaa actatctgct
     5401 gactgacgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa
     5461 gtcttacgag gaagagttgg cgaaagatcc acgtattgcc gccaccatgg aaaacgccca
     5521 gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac
     5581 tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca
     5641 gactcgtatc accaagtaat gctgtgaaat gccggatgcg gcgtgaacgc cttgtccggc
     5701 ctacaaaacc gaaacgtatg taggcctggt aaggcgcgtc agcgtcgcat caggcagttg
     5761 ttgtcggata aggcgtgaaa gccttatccg tcctggaatg aggaagaacc ccatggatgt
     5821 cattaaaaag aaacattggt ggcaaagcga cgcgctgaaa tggtcagtgt taggtctgct
     5881 cggcctgctg gtgggttacc ttgttgtttt aatgtacgca caaggggaat acctgttcgc
     5941 cattaccacg ctgatattga gttcagcggg gctgtatatt ttcgccaatc gtaaagccta
     6001 cgcctggcgc tatgtttacc cgggaatggc tggaatggga ttattcgtcc tcttccctct
     6061 ggtctgcacc atcgccattg ccttcaccaa ctacagcagc actaaccagc tgacttttaa
     6121 acgtgcgcag gaagtgttgt tagatcgctc ctggcaagca ggcaaaacct ataattttgg
     6181 tctttacccg gcgggcgatg agtggcaact ggccctcagc gacggcgaaa ccggcaaaaa
     6241 ttacctttcc gacgctttta aatttggcgg cgagcaaaaa ctgcaactga aagaaacgac
     6301 cgcccagccc gaaggcgaac gcgcgaatct acgcgttatt acccagaatc gtcaggcgct
     6361 gagtgacatt accgccattc tgccggatgg caacaaagtg atgatgagct ccctgcgcca
     6421 gttttctggc acgcagccgc tctacacact cgacggtgac ggcacgttga cgaataatca
     6481 gagcggcgtg aaatatcgtc cgaataacca aattggcttt taccagtcca ttaccgccga
     6541 cggcaactgg ggcgatgaaa agctaagccc cggttacacc gtgactactg gctggaaaaa
     6601 ctttacccgc gtctttaccg acgaaggcat tcagaaaccg ttcctcgcca ttttcgtctg
     6661 gaccgtggtg ttttcactga tcaccgtctt tttaacggtg gcggtcggca tggttctggc
     6721 atgtctggtg cagtgggaag cattgcgcgg caaagcggtc tatcgcgtcc tgctgattct
     6781 gccctacgcg gtgccatcgt tcatttcaat cttgattttc aaagggttgt ttaaccagag
     6841 cttcggtgag atcaacatga tgttgagcgc gctgtttggc gtgaagcccg cctggttcag
     6901 cgatccgacc accgcccgaa caatgctgat tatcgtcaat acctggctgg gctatccgta
     6961 catgatgatc ctctgcatgg gcttgctgaa agcgattccg gacgatttgt atgaagcctc
     7021 agcaatggat ggcgcaggtc cgttccagaa cttctttaag attacgctgc cgctgctgat
     7081 taaaccgctg acgccgctga tgatcgccag cttcgccttt aactttaaca acttcgtgct
     7141 gattcaactg ttaaccaacg gcggcccgga tcgtcttggc acgaccacgc cagccggtta
     7201 taccgacctg cttgttaact acacctaccg catcgctttt gaaggcggcg ggggtcagga
     7261 cttcggtctg gcagcagcaa ttgccacgct gatcttcctg ctggtgggtg cgctggcgat
     7321 agtgaacctg aaagccacgc gaatgaagtt tgattaaggg agataacaaa aatggcaatg
     7381 gtccaaccga aatcgcaaaa agcacgtttg tttattactc acctgctact gctacttttt
     7441 atcgcggcga ttatgttccc gctgctgatg gtcgtcgcta tctcgctgcg tcagggaaac
     7501 ttcgcgaccg gtagcctgat cccggagcaa atctcctggg atcactggaa actggcgtta
     7561 ggttttagcg ttgaacaggc tgatggtcgc attacgccac cgccattccc ggtactgctg
     7621 tggctgtgga actcggtaaa ggtcgccggg atttccgcga ttggcattgt ggcgctctct
     7681 accacctgcg cctacgcttt cgcccgtatg cgctttccag gcaaagcgac gctgctgaaa
     7741 ggaatgctga ttttccagat gttcccggca gtactttcac tggtcgcgtt gtatgcgttg
     7801 tttgatcgtc tgggtgagta cattccattc attggcctga atactcacgg cggcgtaatt
     7861 ttcgcgtatc tgggtgggat tgcgctgcat gtctggacca tcaaaggcta tttcgaaacc
     7921 atcgacagtt cgctggaaga agcggcggcg ctggatggtg cgacaccgtg gcaggccttc
     7981 cgccttgtcc tgttgccgct gtcagtaccg attctggcgg tggtgttcat cctgtcgttt
     8041 atcgctgcca ttactgaagt tccggtcgca tcgctgttac tgcgtgacgt aaacagctac
     8101 accctggccg tggggatgca gcaatacctc aacccgcaaa actacctgtg gggtgacttt
     8161 gccgccgctg ccgtgatgtc tgcattaccg atcaccatcg tcttcttgct ggctcaacgc
     8221 tggctggtca acggcctgac ggcaggtggt gtgaaaggtt aaagatgttg ttctgccaat
     8281 gttatgccgc tgcaccctca acttacgtta tcccaacttg tgactgttat tcggcgctcc
     8341 acggagcgcc tttttttctt tcgtctgcaa tctgaattgt tcgccggtta atatttccat
     8401 catagagctt attattttta cgttatttgt tttcccactt acgataattc tctttcgtgc
     8461 tctgagtcac ggcaatagta ttgttttcat caatttttga taattatcac aattaagatc
     8521 acagaaaaga cattacgtaa acgcattgta aaaaatgata attgccttaa ctgcctgaca
     8581 attccaacat caatgcactg ataaaagatc agaatggtct aaggcaggtc tgaatgaata
     8641 cccagtataa ttccagttat atattttcga ttaccttagt cgctacatta ggtggtttat
     8701 tatttggcta cgacaccgcc gttatttccg gtactgttga gtcactcaat accgtctttg
     8761 ttgctccaca aaacttaagt gaatccgctg ccaactccct gttagggttt tgcgtagcca
     8821 gcgctctgat tgggtgcatc atcggtggtg ccctcggtgg ttattgcagt agccgcttcg
     8881 gtcgtcgtga ttcacttaag attgctgctg tcctgttttt tatttctggt gtaggttctg
     8941 cctggccaga acttggtttt acctctataa acccggacaa cacagtgcct gtttatctgg
     9001 caggttatgt cccggaattt gttatttatc gcattattgg cggtattggc gttggtttag
     9061 cctcaatgct ctcgcccatg tatattgcgg aactggctcc agctcatatt cgcgggaaac
     9121 tggtctcttt taaccagttt gcgattattt tcgggcaact tttagtttac tgcgtaaact
     9181 attttattgc ccgttccggt gatgccagct ggctgaatac tgacggctgg cgttatatgt
     9241 ttgcctcgga atgtgtccct gcactgctgt tcttaatgct gctgtatacc gtgccagaaa
     9301 gtcctcgctg gctgatgtca cgcggcaagc aagaacaggc ggaaggtatc ctgcgcaaaa
     9361 ttatgggcaa cacgcttgca actcaggcag tacaggaaat taaacactcc ctggatcatg
     9421 gccgcaaaac cggtggtcgt ctgctgatgt ttggcgtggg cgtgattgtc atcggcgtta
     9481 tgctctccat cttccagcaa tttgtcggca tcaatgtggt gctgtactac gcgccggaag
     9541 tgttcaaaac gctgggggcc agcacggata tcgcgctgtt gcagaccatt attgtcggag
     9601 ttatcaacct caccttcacc gttctggcaa ttatgacggt ggataaattt ggtcgtaagc
     9661 cactgcaaat tatcggcgca ctcggaatgg caatcggtat gtttagcctc ggtaccgcgt
     9721 tttacactca ggcaccgggt attgtggcgc ttctgtcgat gctgttctat gttgccgcct
     9781 ttgccatgtc ctggggtccg gtatgctggg tactgctgtc ggaaatcttc ccgaatgcta
     9841 ttcgtggtaa agcgctggca atcgcggtgg cggcccagtg gctggcgaac tacttcgtct
     9901 cctggacctt cccgatgatg gacaaaaact cctggctggt ggcccatttc cacaacggtt
     9961 tctcctactg gatttacggg tgtatgggcg ttctggcagc actatttatg tggaaatttg
    10021 tcccggaaac caaaggtaaa acccttgagg agctggaagc cctctgggaa ccggaaacga
    10081 agaaaacaca acaaactgct acgctgtaat cttcctgtcc agcacgccgc gccagttcgg
    10141 cgtgctgtct ttttactccc gcttcagccg tttcgaatta cacagccaca gggtgatcac
    10201 cagtaacagg atcgcagccg agtagatcaa cacatccagt ggcgatttat gatcgacgat
    10261 gatcaagcgc acaatggcgg tgatcccaat atagacaaag taacgtagcg ggaagtgaaa
    10321 accggactga aagtacttca caatcagcgc gataaattcg aaatagagaa agtaaaccac
    10381 cagtccttct accagctcat atttgctggt ttgttctggc gcgaacagca catcagccag
    10441 atgcaccgtt tctttgccga ggaagacaac caaaataagg ccgaggcaca gcaggccaag
    10501 attgagtacg gtctgcaaaa tggtggagat aaactccacg cgcggacgag agagtgacgt
    10561 catagcagta cctccttcac agtggcgagc ttcctgtata acgcaaaaat gtgacggaga
    10621 tctatatttt ttgtgtatgt tttgttcaac ctggatttgg agcgcgcctc caccaaagcc
    10681 ggatatacct acccgtaggc cggataagcc gcgcctggcg gcgcatccgg caagacaaac
    10741 cgcaccaaag ccggtaattc aaaaatcacc cattcaggtg attcccagcc accagcaatg
    10801 cgcgaaaaat cgacaatgtt cctctggaac aaatgaaacg taacctgata aaggtggtaa
    10861 aaatgaagcc ttattttgct gctttgatgt tatcagtctc tgttcttcct gcttatgctg
    10921 gtccgctcgg tactgctgat aaagccgacc taccgcaaag caatgtctcc agcccaatga
    10981 tggcccagtc gctcaggcaa ccagacctgc aaccgatatc gacggacagg aagacagaat
    11041 gttttcgcct ttacacacca gatcgcaaac cgggggtgaa ctgcgttcct gatggctcga
    11101 caggccatta agaatgaagg tgccagtgag tgcgaaaact ggcacctttc ccatgactta
    11161 gcggaaattg acgctacggt cgctggtcat gtcatacaac tggaacttac gcccaagttg
    11221 ctgaccaccg tcacgcgtca gcggcgtcca gccaattgcc gcacggctgc gtgtcgggcc
    11281 agacgagaag agatccaacg gtacagagac atacacgcct ttggtgaagt ccccttcgcc
    11341 gtactcctct tttgaaacat tagtgatcgt ggcatagcca cccaccacca caccgctgtc
    11401 aaagcgtttg gcgatctcca gcgtgccgcc tttatcccct gccagatact gcccgacgct
    11461 ggctttaact aacacatcct gagcgaaaga tggcgtccag taggcggtca gatgtccggt
    11521 tttcacgctg tagtcggtga atttcatcat atcctttgca ctacgccagt cgcgctgttt
    11581 aacgtagttg gcatccacac caaacgccca gttgctgtcc agcgggcgat acaacacttc
    11641 tgcccccgca ccgccaaaca tggtttcgag atagccgccg tagacctgac cgtaaaatcc
    11701 attgcccaga tgctggaagt agttggcttg caggttattc acatagacat cgttctgcac
    11761 atactcgcgc acatgggtac gcacgcgcgg taagtgcgag tcctgcggcg gattggtgta
    11821 gttaaacttg tcgtagttgt tggcaagatt tgcaaacagg ctgccggtgg tcagcagatg
    11881 gtccgtcagc cacaaatctg ccgttcccat cacgcccagc tgatacatgt aaaagttttc
    11941 cgggccaccg accgactggt tcagcaccgg atcgatatgg aaatcaaagc gcgatttgtc
    12001 gatataccag ccctgctcgg tggactgcgg aaccactggc tcgacgcgtt tttgcgccag
    12061 cttcgtttcg tggcccaacg gctctccggc gagatggttt ttcaggccgg cgacatccgt
    12121 ttccgtggtc acctgcggca tgttaaggcg attttccgta acgcggatcg tcttgatccc
    12181 atccggcaga tcgttcatca cgatccgatt agcacggatg atcccttcgc gcgaatcacg
    12241 atatttcacc tgctcgccgg taacatacag cgtatcgcct ttcgcctgga tctgcggatc
    12301 ggcaagtccg gcattgtatt tcaacagcgt taactgattc gccaccaccg aatgctgcaa
    12361 aatggcatcc tgcggctgcg gttgatattg cgggcgggcg ctatcattgt aagacgggcg
    12421 cagatcgtta aagttggtgc gcagcgtgac gccaaacata aaggtgttgc cacgttcata
    12481 gctaaggtta acgtcggccc aatcggtaac gcgataaatc gccccgacgt taaacttact
    12541 tttttgctcc agcttaccgg caaaatcctg ctgataatta ttgccttcat actccagttt
    12601 caggcgcagc ggttgccagg gcgtctggta ttccacgccg ccaaacagtg aggcaggacc
    12661 gtggaacatc tggctaccgt cgatagatcc cgcctgtttg tagctgttat cgcgatagca
    12721 atatttatca ctggctgaac agagcggatt tttcacatta ccgctggtgc ccaaatatcc
    12781 ccagcccagg ccgagcgtaa aatcgaacgg cccccaggct ttgctggcaa caagatattc
    12841 cgcatcaaac agccccgtac cgccgatatc ccgcgcacca accgccactt gcggcagcca
    12901 ataactctct tcccacaaac gcagtttgag atcgaaggct ttatctttat acgtttgatc
    12961 gccagagaac gcttcgacgc tgctgtactg ccgggtgcgc acgtcggtgt agcgcagcgt
    13021 tgtttccagc cacgggaaga gttgcactga agctgagtaa taacggtact gatcgttatc
    13081 gcgatagttc agactcaact ccccttcccg tgccatacgc gcggtgggcg tttgtaataa
    13141 tcctacgcca ccgaaatccg actgcgacgg accaatgggc gcaggatatg tttcgccgta
    13201 gcaagctgtg ctaatgccca gcgccagtaa gctaagcaga tgtctttttt tcattattga
    13261 ggtatccgct gcgtcagagt ctgaagaatg tcggcgttaa gggcatcagg cgtctcactc
    13321 cagacggagt ccgcgaggcc aacataaata atgctgccgg gcatcggctc tacgtgacgc
    13381 ttgttccagt aagccaccgg cgctttttgc gtgcgtccgt ccgggtaaac cacccacgcg
    13441 tagctgcgat ccgcaccgct gagcaggctc tggtcagaga gatagctcgc cacgtcgcga
    13501 cctggagtga atggctgctt gccaggacgg ctgataagcc cgaacaacgt gaccgtggac
    13561 ggcggtggtc cgacccacag cgtgtagttg ccctgcaacg gcgggttacc gcgttcggca
    13621 acgcggacga tatcgggatc aagattgatt ttttgcctac ccgtcacttt caacgcctga
    13681 atttgctggc gtaaggcgtt aatcgcagcg gcatcctcag cgctggagtc tgcggccagt
    13741 tctgccagcc gtgtcagcaa cgcctgctgc tgacgcaatg ctgccgccgt tgccagctct
    13801 tcgctgatca ccgcaccggg ccaccagctg tttgccagcc gcggttggcc taccagatcg
    13861 attaaatgct ctgcgcctgt tagtgttttg gcctcactgc tgccattgct gaacaccttg
    13921 acggttcctg ccgcgaagac cgatgacgct cccacactca aaagcaacgc gacaatagtt
    13981 tgtttaatca ttgttttgcc gccttgatga gagtggtttt caccgggaaa taatccgcgc
    14041 cgagatattg ttccgactgg cgaatttgcc cttcgctgtc gatccaatag cggttatgcc
    14101 agcgcgcctg gtcggtggag acttcttcgt ccagcacgcg aaccgcggtt tcatcgctgc
    14161 cgaccttaac ggtatcggtg ccatcccatt tgaagactga gcgtgcggtg gcgtagcgta
    14221 cctgcttata ctcggtccag cccatcgtgc gcgtccaggt tgcaccatca atgatttgtg
    14281 cgggtttaat cagcgggtcg gcggcaaggt tattcacctc aatcaagttg tcgccgccaa
    14341 gcagagtctt caccagacga ccatgttgtg tgacgagagt ggcctgatcc tgagtgaccc
    14401 atttttgttg tccgtcttca gcgaaggcca gcaccacaaa caactgcggc ccgccattaa
    14461 gctgcatgta ctggctggcg tagggcatat tttgaatatc atcgtccgtc agctgtacac
    14521 ctggcgtacc gaacagactg tcccacagtg aattgcccag ctctttagtc gtggctgaac
    14581 aggcctgtaa tagcaggcag ataagaatga gtgcaggtcg cttcacgact cttctccaga
    14641 ggggcagaat aaccacaccg gagtgtggtt atggtaaatc ccttcatact tcaagccaca
    14701 gatgcgatga ctcgaattat tttggggata catactttat tactgggtac tggtggtcgt
    14761 ggttgttgtg gtcccggtat tagaaccatc accgccaccg gttgccgcga gcgcgacacc
    14821 caccgccgag cttacggtgc tgacgctggt cgcggaggaa ctacccgccg aaaccgacgt
    14881 tgctgccgac cctgccgctt cgcccacctg tacaggtgca gcccacacag aagtcgccgc
    14941 aagcgcagat atggcaaaaa tgccatacag aacttttttc ataacaattt cccttcattg
    15001 aatgaatgga gatttaccca aaaacatttc gggcgggatc gagtatacac aactaaagca
    15061 tgcgaattac ggcgcgggga attagagatg gggttttagg ataattggaa tgaatatcaa
    15121 atataagcta caacttatat ataaataaca ttaaaaatca tagtgttaaa a
//
LOCUS       DAZFFG010000109        14849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFFG010000109 DAZFFG010000000
VERSION     DAZFFG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.109"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(654..1190)
                     /gene="rcdA"
                     /locus_tag="ACHJ7J_003836"
     CDS             complement(654..1190)
                     /gene="rcdA"
                     /locus_tag="ACHJ7J_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO5002013.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            1274..2482
                     /locus_tag="ACHJ7J_003837"
     CDS             1274..2482
                     /locus_tag="ACHJ7J_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5002014.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            2482..3297
                     /gene="ybjI"
                     /locus_tag="ACHJ7J_003838"
     CDS             2482..3297
                     /gene="ybjI"
                     /locus_tag="ACHJ7J_003838"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO5002015.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVAVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            3383..3667
                     /gene="ybjH"
                     /locus_tag="ACHJ7J_003839"
     CDS             3383..3667
                     /gene="ybjH"
                     /locus_tag="ACHJ7J_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO5002016.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3708..4940)
                     /gene="mdfA"
                     /locus_tag="ACHJ7J_003840"
     CDS             complement(3708..4940)
                     /gene="mdfA"
                     /locus_tag="ACHJ7J_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO5002017.1"
                     /translation="MQNKLASGARLGRQVLLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            5225..5821
                     /gene="ybjG"
                     /locus_tag="ACHJ7J_003841"
     CDS             5225..5821
                     /gene="ybjG"
                     /locus_tag="ACHJ7J_003841"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5002018.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQATGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5879..6637
                     /gene="deoR"
                     /locus_tag="ACHJ7J_003842"
     CDS             5879..6637
                     /gene="deoR"
                     /locus_tag="ACHJ7J_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO5002019.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6684..7886)
                     /gene="dacC"
                     /locus_tag="ACHJ7J_003843"
     CDS             complement(6684..7886)
                     /gene="dacC"
                     /locus_tag="ACHJ7J_003843"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5002020.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            8133..8759
                     /gene="gstB"
                     /locus_tag="ACHJ7J_003844"
     CDS             8133..8759
                     /gene="gstB"
                     /locus_tag="ACHJ7J_003844"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO5002021.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(8756..9871)
                     /gene="yliI"
                     /locus_tag="ACHJ7J_003845"
     CDS             complement(8756..9871)
                     /gene="yliI"
                     /locus_tag="ACHJ7J_003845"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO5002022.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FVALGENNQRSTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPRWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(9982..10365)
                     /gene="bssR"
                     /locus_tag="ACHJ7J_003846"
     CDS             complement(9982..10365)
                     /gene="bssR"
                     /locus_tag="ACHJ7J_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO5002023.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            10578..11903
                     /gene="rimO"
                     /locus_tag="ACHJ7J_003847"
     CDS             10578..11903
                     /gene="rimO"
                     /locus_tag="ACHJ7J_003847"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO5002024.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(11950..13278)
                     /locus_tag="ACHJ7J_003848"
     CDS             complement(11950..13278)
                     /locus_tag="ACHJ7J_003848"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5002025.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNIGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(13286..>14849)
                     /locus_tag="ACHJ7J_003849"
     CDS             complement(13286..>14849)
                     /locus_tag="ACHJ7J_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO5002026.1"
                     /translation="PNNALFEIYFYYPAGNLLNASDKLFYLPFAFIIIVLLVVYLMTT
                     RVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERS
                     NKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRK
                     LCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKS
                     AICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAM
                     EIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQ
                     IIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCV
                     EITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFT
                     GDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPF
                     SAWDKSGKLVKE"
BASE COUNT         3807 a         3731 c         3492 g         3819 t
ORIGIN      
        1 gggaatgttc cttcataagg atcatcaaag agatcagatc gacatagaaa aattttttga
       61 tacgtgacta atgccatgaa tttagtaaaa tccatatatt tccacaggga aatatcatct
      121 gaactaggtt gaggaaaaca tgggtgatta tcgtatttag ccatttatat cattccattc
      181 aaaaaaagtt aatataatgt aacctaagac tttaaaattc tctgatttac tattatatat
      241 cacgcgcaat ctcataatag ttaaggagcc attttagacc ttttgagcta caaaaaaaat
      301 agcaagatga ccactcgcgg tacttagcgg ttgttctaca tgtgcgttca tgtgccccga
      361 ttcttggagg taggttaaaa aagctccccg cacccagcaa cagtaatatg gcatagtcct
      421 tttgaaggag ttaaagatat acggaacaca gagtaagtta gaggagaaag gcccattacc
      481 ttcaaagtaa ggccagaacg ataacacagt ggcgacaaag tggcggcagc ggttggcatt
      541 accccgtaat aaccaccact gaccaccaat ctaacttatt gtttctaatc taacttattg
      601 ttttcattaa cccgtttaca taaatgggtt ttttgttgcc tgaattttat ctactaccca
      661 gcaaccctct caaccatcct caaaatctcc tcgcgcgata gcggcttacg gtcggtgaca
      721 aaatgcagcg tcatcccctc aataaacgca tcaagcgcgc gggcggttcc gggttcaaac
      781 cattgttcga gcgtttgctg actgcgctgc atccagtttt gcattaccgt ttttaatagc
      841 ggttttcggc tagccagcgc gtagagctgg tacatcagct ccatgttatc cggcgttgca
      901 acctgtgagc tgtagatcat atcggtgata gcctggcatg cgccttgagc atcactaaca
      961 tcgctaaaaa atgcctgata ttgccgggac atgatctcag taaaactgct gaacgcctcc
     1021 agcaacaact catcaattcc tgaaaaatag taggtcatcg atcccaacgg taccccggca
     1081 agggtagcaa ttttgcggtg cgtaacagca tgtattccgt aaagtttcac cgcctccagc
     1141 gtggcctgga taattttttc tcgccgttgc ggatcgttag cgcgacgcat gtcatttcct
     1201 ctgtctgcaa ttgtgtacaa atgtacacaa ccttgctaat gttgtcttcc ctcttcttat
     1261 tttgacgtga tctatgaccg taaattcttc acgtaatgca ttgaaacgcc gaacctgggc
     1321 gctgtttatg ttcttctttt tgccgggcct gttaatggcg tcctgggcaa cccgtacgcc
     1381 tgctatccgc gatattctct ctgtctcgat cgctgaaatg ggcggtgttc tctttggtct
     1441 gtcgatcggt tcgatgagcg gtattctctg ctcggcgtgg ttagtgaaac gctttgggac
     1501 acgtaatgtc atcctggtca ccatgtcctg cgcattggtc gggatgatga tattaagtct
     1561 ggcactctgg ctgacatcgc ccctgctctt tgccgttggt ctcggcgtct ttggggcaag
     1621 ttttggttct gcggaagtgg cgataaacgt tgaaggtgcc gccgttgagc gagaaatgaa
     1681 taaaacggtt ttgccgatga tgcacggttt ttatagcctg ggcacgctgg caggcgctgg
     1741 tgtcgggatg gcactgacgg cctttggcgt tccggcaacg gtgcacattt tattggcggc
     1801 gctggtaggc atcgcgccta tatatatcgc cattcaggca atccctgacg gtacgggcaa
     1861 aaatgctgcc gatggcaccc agcatggcga aaaaggcgta cctttttatc gcgatatcca
     1921 gttgctgctg attggtgttg tggtgctggc gatggccttt gccgaaggtt ctgccaacga
     1981 ctggttaccc ttattaatgg ttgacggtca cggttttagc cccacttccg gctcgctgat
     2041 ttatgccggt tttaccctgg ggatgaccgt tggacgcttc actggcggtt ggttcatcga
     2101 ccgttacagt cgcgttgccg tggttcgggc cagtgcgcta atgggggcgt tgggtattgg
     2161 gctgattatt tttgtcgata gcgcctgggt cgctggggtg tctgttgtac tctggggact
     2221 gggtgcctcg ctgggcttcc cgctgaccat ttctgccgcc agcgataccg gccccgatgc
     2281 accaacccgc gtcagtgtgg tagcaacgac cggttatctg gctttcctcg tcgggccgcc
     2341 gctgctgggc tatctcggcg aacattatgg attacgtagt gcaatgctgg ttgtactggc
     2401 gctggttatt ctcgcggcta ttgtcgcgaa agccgtcgcc aaacccgata ccaaaacgca
     2461 gacggcgatg gagaatagtt gatgagcatt aaattaattg cggtagacat ggatggtact
     2521 ttcttaagcg atcaaaaaac ctataaccgt gagcggttta tggctcagta tcagcaaatg
     2581 aaagcacaag gcattcgctt tgtggtcgcc agcgggaatc aatattatca gttgatctct
     2641 ttcttccctg aaattgctaa tgaaattgcc tttgtggctg aaaacggcgg ctgggtagtg
     2701 agtgaaggca aagatgtttt taatggcgag ctgtcgaagg atgcgtttac tactgtcgtg
     2761 gaacatttgc tgacgcgccc ggaagtggaa attattgcct gcggaaaaaa tagcgcctat
     2821 acgctcaaaa aatatgacga tgccatgaaa acggtggcgg aaatgtatta tcaccgtctg
     2881 gaatacgtcg ataactttga caacttagaa gatatcttct ttaagtttgg cctgaatctt
     2941 tccgatgaac tgattccaca agtacaaaaa acattacatg aggccatcgg cgatattatg
     3001 gtgccggtcc acaccggcaa cggcagcatc gatctgatta tccccggcgt acataaagcc
     3061 aatggccttc gccaactgca gaaattatgg ggaatagacg acagcgaagt ggcggtcttt
     3121 ggcgatggcg gtaacgatat tgagatgctg cgtcaggcag gctttagttt tgcaatggaa
     3181 aatgccggca acgcggtcgt cgcagcggca aaataccggg caggctccaa taaccgtgaa
     3241 ggcgtactgg atgtgatcga taaagttctt aaacacgaag cgccatttga ccaataaaaa
     3301 cggcagcacg cccggcaaca caatacctga atgaaggctg cataattcgc ggaaattaac
     3361 ccgctgcatt atttcaggcg aaatgtttat gaaaaattgt ctactgttgg gcgcgctttt
     3421 aatgggcttt actggtgtgg cgatggcgca aagtgtcacc gtcgatgtgc cgagtggata
     3481 caaagtggtt gtggttcctg attcagtcag cgttccacaa gcggtcagcg tcgcgactgt
     3541 gccgcaaacc gtctatgtgg ctcctgcgcc agctccggct taccgcccgc atccttatgt
     3601 tcgccatctt gccagcgtag gtgaagggat ggttatcgaa caccagattg acgaccatca
     3661 ccattaattc gagaatgcct gatcgcacaa agcaatcagg cattttttta cccttcgtga
     3721 gaatttccca tctgtttatc ttttaaaaag ataaccatca gcgacagcca caaaattccg
     3781 ttgacaaggt tgaagagatt aaacagtccg ttgcccccgt tcagccaggc atgtttgctg
     3841 atttcaatac cgacggtaaa gatcagcatt tgcagcattc ccatcgccgc agaaaccgta
     3901 cctttactca tatcgctggc aaacagggtt aatcgcacca gtcccgcatt cgccagacca
     3961 ataccgaaag cataaatact taacccggcg gtcatccata aatacgcgtg cgatgagata
     4021 accgttgccg cagcagcgac caacagacca atcataatcg gccagccgcc cataataatc
     4081 agcgaacgta cggtgcggcg cgaggtcaga cgcgctaaca gcaagttacc cgcaattaac
     4141 gccccgaaaa taggcacttg cagcaaacca tattcatagc tgctcaactg ctcgccggta
     4201 atgatgataa tcggcgactg ggcgatccac gccagcaacg gcaggctaac gaatcccagc
     4261 gccagcgccc ccgccacaaa gcgaccgttc ttcagcacca gcttatagtc acgaccgagt
     4321 tctttcagcg acagtttctc gcctatacgc gtggcggttt caggcatggc tcgttgcaga
     4381 ccgaaaaagg agatcgctgc caatgcggca aacaagacaa acatcccttc ccagggcagc
     4441 acatggatcc acgccgcgcc caccagtgga ccaagtagcg gagcaatcag cgccacgttc
     4501 gccatcagcg cggtgatctt gatacaaacc gcctcttcga aggattcctg aattgcggcg
     4561 tatcccacag cgccaatgaa acagaggctt atgccctgca agaagcgcaa cagggtgaat
     4621 tgttcaatgt tttgcgccag taatattgcc agacaggtga caataaacca caccacaccc
     4681 gccagcatca ccggccggcg accaatacga tccgacaacg gccccaggag ccattgtaaa
     4741 aacatcccgc ccgccagata cgcggtcatc gaagtaggaa cccaatcaat gcccgcctga
     4801 tattgttcca ccacggccaa cataccgggt tgaatcatat cgttgccgat ataggttgaa
     4861 aattcgtaaa gcaccagaca gagagggaaa agtaacacct gacgtccaag cctggcaccg
     4921 gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcaa agtttaataa
     4981 aagcgcagct aacgagaaag cgaattttgt agctgaaacc gcggttaagc acattcttac
     5041 attattgcga gtatagctac gctttcttta agttttattt aacctatgcc cgttacaata
     5101 acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc
     5161 tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga
     5221 agttatgctg gaaaatttga atctctctct attctctctt attaacgcga cgccagactc
     5281 ggctccgtgg atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc
     5341 gttgctggcc gcggtacttt ggttgtgggg gcttacagca caacggcaac tggtgataaa
     5401 aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc
     5461 gcacgaccga ccatttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga
     5521 ctcattcccg agcgatcacg gtacggtgat tttcaccttt gcactggcat ttttatgctg
     5581 gcatcgcctg tggtccggct ctcttttaat ggtgctggcc gtcgtcattg cctggtcgcg
     5641 cgtttatctt ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat
     5701 tggctgcctt agtgcccaga ttatctggca agcgacaggg cataaactct atcaacgtct
     5761 gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg tgcgtgactg
     5821 atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat
     5881 ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa
     5941 gttacatctt aaagacgccg ccgcgctgct tggggtttcg gagatgacga ttcgtcgcga
     6001 tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgcg
     6061 cagtgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg
     6121 ggcggcaaaa ctggctgcga cgctggtaga acccgatcag accctctttt ttgactgtgg
     6181 caccaccacg ccgtggatta ttgaagcgat tgataatgaa atacctttca ccgccgtttg
     6241 ttattcgcta aatacctttc tggcgctgaa agaaaaaccc cattgccgcg catttctgtg
     6301 cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc agcaaacgtt
     6361 gaataatttt tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg
     6421 cgctacctgt tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca
     6481 aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtgcgtccgg cgcgcatggg
     6541 tgacctgaaa cgctttgata ttgtggtgag tgattgttgt ccggaagatg agtatgtgaa
     6601 gtacgcgcag acgcaccgta ttaagttgat gtattaatga agtataaccg gatgacgttt
     6661 cgcaccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc
     6721 atcatcacga aatcccacat ccgaccaaag aatccgccct cttccacatt ttccatcacg
     6781 atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt gccgacaacc
     6841 tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg
     6901 tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc gagattcact
     6961 tcgctcttat caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc
     7021 gtcacggttt caaagaagcg gaaaccccag gtcaataatt tctcagactc attaaaacgg
     7081 atacggtcag ttttcgcccc cagcactacg gagattaaac gcatatcgcc ctgggtagcc
     7141 gaagcaacca gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc atcaacattc
     7201 agattgctgc tccacagcag acggttacgg ttaggctgac gaattttgtt gaaggtgaac
     7261 tctttctctt tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc tttacccagc
     7321 aatgccatat cgcgcgcggt actgaactgc cccggcgcat ccagaccgtg caccgtctgg
     7381 aaggtagtgt tggtcagacc cagttttttg gcataaccat tcatcaaacc aataaatgac
     7441 tcctggctcc cggcaacgta atcggccagc gcaatacagg cgtcattacc ggactggata
     7501 atcacacctt tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag gaacattacc
     7561 gatgaaccac gcagtgccgg atttcccgtc gcccaggcat ctttaccgac cgtcaccata
     7621 tcggtgagtt taatcttatc ggccttaagc gcctgcccaa ccacatagct ggtcatgatt
     7681 ttagtcaggc tcgcgggatc cagtttctca tccgcattgc cttctgccag cactttaccg
     7741 ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc aacggtttgt
     7801 tccgccgcga atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc tgcaagacca
     7861 cgaaggagag aggagtattg cgtcataaat gccacccgag tatccattca aaaaaaatac
     7921 gttatatcgc cgtatgataa gttcggtgat taataacgca cacacaaact taaagaaacc
     7981 gtaagttggt aaagttttta aagtttatgc aatatcctgc cgcttcgctg cttctggtgt
     8041 catttttttg ccagctgttg cttaaaagtt aactttactt caccatcgga gtcctgttca
     8101 gcctgtggct gcctgtcgga aaaggggtga gtatgattac gctgtggggt cggaataatt
     8161 caactaacgt aaaaaaagta ttgctgacgc tcgaagaact ggaactacct tatgagcaaa
     8221 ttctcgcggg ccgtgagttt gggataaatc acgatgctga ttttctggcg atgaacccta
     8281 acgggctggt gccgttgttg cgtgacgacg aaagtgatct cattctttgg gaatcaaacg
     8341 ccattgtccg ctatctggcg gcacagtacg ggcaaaaacg tctgtggatc gactcaccgg
     8401 cacgtcgtgc ggaagcggaa aaatggatgg actgggcaaa ccagacactc agtaatgctc
     8461 atcgcgggat cctgatggga ttagtcagaa caccacccga agagcgcgat caggccgcca
     8521 ttgatgccag ttgcaaagag tgcgacgccc tgtttgccct gctcgatgcg gaactggcaa
     8581 aagtaaaatg gttctctggc gacgagtttg gtgtgggcga tatcgctatc gcaccgttca
     8641 tctacaattt gtttaacgtt ggcctgacct ggacaccgcg tccaaatctg caacgctggt
     8701 atcagcaact cactgaacgc cccgcagtgc gcaaagtggt gatgattccc gttagctaat
     8761 tgcgtgggct aactttaagt aattccccac tggactcgtc ggtgagaacg tataaataac
     8821 cgtcgggtcc ggtgcgaaca tcacgaattc gctgccctct gtccgttaaa atacggccat
     8881 cttctgtcac tttgtcgccg ttgacgctca tcacaatgac atctttatct ttcagcgcgc
     8941 caataaataa tttttgctgc caccggggga atttatcgct gttatagaag gccatgccgc
     9001 tcacggcggg cgaatctttc cagtaaaaaa caggttgctc ggtccccgcg acgatctccc
     9061 ctttcgcttc cggtatctta aagcctgaat agttgattcc ccaggttgcc agcggccagc
     9121 cgtagttttt gcctttttgc gggatattaa tttcatcacc accgcgcggg ccatgttcat
     9181 tcagccacag tgcattactc cacggattca tcgccattcc ttgcggatta cgaatgccat
     9241 aagaccagat ctcggcgcgc acaccggatt cctttataaa aggattatca tccgggattt
     9301 cgcccaggtc ggtcagacgc accagtttgc cctgtaattt atccagatcc tgcgccgtcg
     9361 agcgctgatt gttttcgccc agagcaacaa aaagataacc tttaccgtcg aataccagcc
     9421 gcccgccaaa atggttgccg gtagacagtt ttggcatctg gcgaaagacg gtgcggaagt
     9481 cggtcacttt tgagagatca tcacttaagc ggccataccc cacagcagtt ccggctttgc
     9541 catcatcgcc aacttcggaa taacttaacc agatgcggcg agactgagca aaatcaggcg
     9601 ctaaaaccac gtccagcagg ccgccctgcc cgtgcgccca aacgtccgga actccggaaa
     9661 gcggcgcaga taatcctttt cctgcttgcc agtgacgcaa ctcgccgccg cgcagagtga
     9721 ttaacatacc gtgattatcg ggtaaaaagg ccagtgccca gggatggtcg agtttgtctt
     9781 gcagtacttc gacatttacc gttgcaggag ccgcccagag agcggaagaa agacaaataa
     9841 ggggcacaag gaaaaagaat tgtcgatgca taacgcctcc ggattgactc tggcttaaag
     9901 cgtagtcagt ggaggagata acaaattcat ttttacaaaa acttaaacat gaagggggag
     9961 acgctttctc ccccttagtt ttcaggcctt ctcaagcatg gcgtgcttct gcaggctctg
    10021 gatactcagc gttaagctca tcagacaatt ttcaagctta tcggcgttga cggtaataac
    10081 agtcgggcaa tcatggtgcc cactcatcaa acatactgcg gctgtcgcta atgcttcttc
    10141 agcatgatga agagcactcc actcttcctg atccagatga agattcaacc gcagcgattt
    10201 atcgtgcagt tcgcgattca gtttaaaaaa gttatctcgt agatgattgc tttcgctgac
    10261 ggacatgtat ccttttgcct tcctcagttg cacgtatgcg gcgaggtcaa cgcgtgcgtc
    10321 gatcaaccgg ttcagcagat cgattcgctg tctgtcaacg aacatacttc gatcctcctc
    10381 tttccagcct tgttgcaatt taatagtatg gctatttttt gagcaaaatg ctgtccagga
    10441 tcaattcttc acgccgaatt aacaaaattt acatttcctt cgcctccgcc agcgaatgag
    10501 attgcgatac aaattttatc cagaactctg tagaatccct gccctgactt ttcctgtaac
    10561 tgaatatttt ttaagctatg agcaaagtaa ctccccagcc gaaaatcggc tttgtttccc
    10621 ttggctgtcc gaaaaacctt gttgattcag agcgtattct caccgaacta cgcactgaag
    10681 gttatgacgt ggtaccgagc tatgacgatg cggacatggt gatcgtcaac acctgcggct
    10741 ttattgacag cgcggtacaa gaatcactgg aagccattgg tgaagcgttg aatgaaaacg
    10801 gcaaggtaat tgtgaccggt tgtctggggg caaaagaaga tcagatccgc gaagtccacc
    10861 cgaaagtgct ggaaatcacc gggcctcata gctatgagca ggttctggag cacgttcatc
    10921 actacgtgcc aaaaccgaaa cacaacccat tcctgagcct ggtgccagaa caaggtgtca
    10981 aactgacgcc gcgtcattat gcctatctga aaatttctga aggctgtaat caccgctgca
    11041 ccttctgcat tattccgtct atgcgcggcg acctggtgag ccgtccgatt ggcgaagtgt
    11101 taagtgaagc gaaacgtctg gtagatgcgg gcgttaaaga gattctggtg atctcgcagg
    11161 atacttccgc ctatggcgtt gatgttaaac atcgtactgg cttccacaac ggcgagccgg
    11221 taaaaaccag catggtcagc ctgtgcgaac agttatcgaa actggggatc tggacacgtc
    11281 tgcactacgt ttacccttat ccgcatgtgg acgacgtcat cccactgatg gcggaaggca
    11341 aaatcctgcc gtatctggac attccgttgc agcacgccag cccgcgcatt ctcaaactga
    11401 tgaagcgtcc gggttctgta gatcgccaac tggcgcgcat caaacagtgg cgcgaaatct
    11461 gcccggaact gaccctacgc tcaaccttta ttgtcggctt ccctggcgag acggaagaag
    11521 atttccagat gctactcgac ttcctgaaag aagcacgtct ggaccgcgtt ggctgcttta
    11581 aatacagccc ggttgaaggt gcagacgcca atgccctgcc tgaccaggtt ccggaagaag
    11641 tgaaagaaga acgctggaac cgtttcatgc agctgcagca gcagatttcc gccgagcgcc
    11701 tgcaagagaa agtgggccgt gaaattctgg tgattatcga cgaagtggac gaagaaggcg
    11761 cgattggtcg cagcatggca gatgcaccgg aaatcgacgg cgcggtttat ctcaatggtg
    11821 aaaccaacgt taagccgggt gatatcctgc gtgtgaaagt cgagcacgcc gatgagtacg
    11881 atttgtgggg tagccgggtt taattaattc aggccgggca aaatcatttg tccggcctgc
    11941 gaataatgat tactgccagc taatctgtgc cctgccgttt ttcttactct tatacagccg
    12001 ctcatccgcc agcattttca agcgttcgac ggtagtacat tcatttgaat gcgctacacc
    12061 gatactggtg cttaaattaa tgcttcccac ctggaaatga acgatttctt ttagaatcga
    12121 tagcaactct tccagcgttc cacccgtata aatcaccgca aattcgtcac cgccgaagcg
    12181 gtagacttta cctttatcta ccgggatctg tgatttaagg attttcacca ctttcatcaa
    12241 cacttcatca cccagcaaat gaccgaaggt gtcgttaatg tttttgaatt tatcgacgtc
    12301 aaacaggcac aaataaccgc ctttttccgg cagcgctttt aaatcttcgt caaaggattt
    12361 tctgcccatt gctttggtca ggtcgtccag gaacgccgag ttatacatac tgtagagttt
    12421 tttgacatcg gcagccgaaa agaaaagccc gccatccagt acgcgttcaa aatggttatt
    12481 aataatggct tctttctcca caataccacg attaatcact ctgcgtacca gatagaggca
    12541 actggtaccc gatatcactg ccaccaatgc ggcaaagaaa ataatcaatg tacaaacccg
    12601 caaataaaca tcttttttat acgcaaatac gtacaatccg gtcggcaaat aggcgaagtg
    12661 ctccatcgct tccgaaacgg tgaagatggc gtttttagca ttatattcat caccggtcac
    12721 cggtttaccg ttcttaaaca gagagaacat caggttctcg ctatccatcg ccagagattt
    12781 tgacagtaaa gaaaaacgct ccagaccaag caaaaatatc agactacgat tgtgagttac
    12841 cggatagagg taatagacga aggttttacc gtcaaaagag ataaacccta tatttttgac
    12901 atgtcgatac ttcacaacct gcataatatc tgcacaataa tttttctcga actcctttac
    12961 ctgaatacat tcgtcactgg aaatcagata aggaccgaca tcgtacaaat aagagggtga
    13021 aaccgagtcg gcgtaagctg tgcgcaaacc aaccaacagc tgttcctccg tgggatttag
    13081 cgtgttgata agatcaattg ccacgctacg ccgatattca gcaatatttg ccaccgcaaa
    13141 agcagtatca ataacggact gtttcaggct atacacccgt tcctttacca tttgagtgta
    13201 gatcccgcag gcaactgctg aaatcatgat aaaaatcagc agactgactg taagtacgaa
    13261 cttattgatt ctggacatac gtaaactact cttttactaa ttttccactt ttatcccagg
    13321 cggagaatgg catgggtttc tggtacagat acccctgagc ctgaacacat cctaatttga
    13381 ttaattcgcg cgcaacgtct tcgctctcta cgccttccgc aatcaccggg atccggttat
    13441 agcgtgccag atcgaaaata atgcgcacaa tttttcccgc tgtaccgaaa atatcgccgg
    13501 taaattgcga gtccaccttt atgctgtctg gattaatttc ataaaaacgt ttcaggtttg
    13561 acaaaccggt gccaaagtca tcaatcgata tccgtacccc ttgtttacgc agttgttcaa
    13621 tattgagata aaaatgttca ttaattcgct cgatcacggt ttcagtgatc tcaacacaga
    13681 ggctgttcgc cttcaggcca ttttctttca tgatggtggt aaaacgctca tgaaagtgcg
    13741 gttcgtttaa ctgccagggc gaaacattaa tatgcagtaa gaaatcatcc ttcagcgacg
    13801 cacgctgact aaaatgtctg aattcaccca ttgccgtttt aataatctga taacccagct
    13861 cattgataaa cccgatatct tcagcaatgg taatgaactt cagcggtgaa atgatccccc
    13921 gctccgcaga cacccaacgg cacaacgcct ccgcgcccag aatggcgcgg gtgttaatat
    13981 caacgatcgg ttgatagacg aggaatagtt cgccaatttc cattgcatgg cgaatattac
    14041 gcgcaatcca gatattctct tctatttcgt tgagtttcgc gtcatcacag aaaataaact
    14101 cggagaacat atggtcacgg atactggaaa tcgccagttt cagtttttct gcgtaactcc
    14161 aggcgttttc cccctgcaaa ggttcacaaa tggcagactt atggatgcgc agattgcggt
    14221 agccaaagct gctgtcaaaa tcgtttacca tcgctatttt acgggtaaat gtctgtacat
    14281 tttcccgaca cagaagtaaa tagagatcgg cattgaatgt cacgatatcg cagcattgcg
    14341 catatttctc ggcaattcgc tgacacaatt tcctgacgat tttattgcgt tcatcaacac
    14401 caaacaccgc ttccacggct tccagaccac acagtttgat gatccccaca gccagatact
    14461 gattattgtt ggactcaata atcgccatat ttttaataaa accgctttcc tgatcgtaag
    14521 agatcagtga gagcaattta tttgaccgtt caatttcggc atctttcatt tccgcgatcg
    14581 aatttttgat gctgataatc tctttcgcat cgccttcgcg aattttcaga gcctcgatct
    14641 gatccgttga gtcgggcaaa aatgccagcg tattaaccag ttcggtcatt tcagaaaatt
    14701 gccgacggaa cacacgagtg gtcattaaat aaaccaccag caatacgata ataatgaaag
    14761 caaacggcag ataaaaaagt ttatctgacg cgttcaataa attaccggca ggataataaa
    14821 aatagatttc gaataatgcg ttgtttggc
//
LOCUS       DAZFFG010000110        14643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFFG010000110 DAZFFG010000000
VERSION     DAZFFG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.110"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(270..695)
                     /gene="arsC"
                     /locus_tag="ACHJ7J_003850"
     CDS             complement(270..695)
                     /gene="arsC"
                     /locus_tag="ACHJ7J_003850"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO5002027.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDQL
                     VKLIADMGLTVRALLRKNVEPYEELGLAEDKYTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILLDAQKGAFSKEDGEKVVDEAGNHLK"
     gene            complement(1028..1201)
                     /locus_tag="ACHJ7J_003851"
     CDS             complement(1028..1201)
                     /locus_tag="ACHJ7J_003851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002028.1"
                     /translation="MHPLTHPLPVTVHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHCQSENASELTV"
     gene            1229..1312
                     /gene="dinQ"
                     /locus_tag="ACHJ7J_003852"
     CDS             1229..1312
                     /gene="dinQ"
                     /locus_tag="ACHJ7J_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO5002029.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1366..2718)
                     /gene="gorA"
                     /locus_tag="ACHJ7J_003853"
     CDS             complement(1366..2718)
                     /gene="gorA"
                     /locus_tag="ACHJ7J_003853"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO5002030.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2790..3632)
                     /gene="rlmJ"
                     /locus_tag="ACHJ7J_003854"
     CDS             complement(2790..3632)
                     /gene="rlmJ"
                     /locus_tag="ACHJ7J_003854"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO5002031.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3835..5877
                     /gene="prlC"
                     /locus_tag="ACHJ7J_003855"
     CDS             3835..5877
                     /gene="prlC"
                     /locus_tag="ACHJ7J_003855"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO5002032.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5885..6637
                     /gene="rsmJ"
                     /locus_tag="ACHJ7J_003856"
     CDS             5885..6637
                     /gene="rsmJ"
                     /locus_tag="ACHJ7J_003856"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO5002033.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6686..8155)
                     /gene="dtpB"
                     /locus_tag="ACHJ7J_003857"
     CDS             complement(6686..8155)
                     /gene="dtpB"
                     /locus_tag="ACHJ7J_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO5002034.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            8321..8374
                     /locus_tag="ACHJ7J_003858"
     CDS             8321..8374
                     /locus_tag="ACHJ7J_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002035.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            8352..8405
                     /locus_tag="ACHJ7J_003859"
     CDS             8352..8405
                     /locus_tag="ACHJ7J_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002036.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(8472..8906)
                     /gene="uspA"
                     /locus_tag="ACHJ7J_003860"
     CDS             complement(8472..8906)
                     /gene="uspA"
                     /locus_tag="ACHJ7J_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO5002037.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9297..9632
                     /gene="uspB"
                     /locus_tag="ACHJ7J_003861"
     CDS             9297..9632
                     /gene="uspB"
                     /locus_tag="ACHJ7J_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO5002038.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9703..11202)
                     /gene="pitA"
                     /locus_tag="ACHJ7J_003862"
     CDS             complement(9703..11202)
                     /gene="pitA"
                     /locus_tag="ACHJ7J_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO5002039.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11434..12636
                     /gene="yhiN"
                     /locus_tag="ACHJ7J_003863"
     CDS             11434..12636
                     /gene="yhiN"
                     /locus_tag="ACHJ7J_003863"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO5002040.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(12951..14003)
                     /gene="yhiM"
                     /locus_tag="ACHJ7J_003864"
     CDS             complement(12951..14003)
                     /gene="yhiM"
                     /locus_tag="ACHJ7J_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO5002041.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDETPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            14387..>14643
                     /locus_tag="ACHJ7J_003865"
     CDS             14387..>14643
                     /locus_tag="ACHJ7J_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002042.1"
                     /translation="MKVFHNIFKYISSNHQDKHSDKVNNHQHHGKVDKKHRSEIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKIWFYN"
BASE COUNT         3765 a         3669 c         3792 g         3417 t
ORIGIN      
        1 atccagtacg tctttaaact gctgcactgt atcgcggata actaagcagc tcactgatac
       61 accatgctgg acgcgataat tggaatctca cgcagggagt aatgtgtaaa tagcaaaatc
      121 gcctgatgcg ctacgcctgt caggcctaca aataacatac tattcatcga attttcataa
      181 ctttgtaggt cggataagac gcgccagcgt cgcatccgac ataaataaag cgcacttttc
      241 taacaacctg tgggggggat atagccgctt tatttcaggt gattacccgc ttcatcaacc
      301 actttctcgc catcttcctt ggagaatgcg cctttttgcg catctagcag aatttccagc
      361 acgacttctg aagggcggca cagacgagtt cccagcgtcg tcaccacaat cgggcgatta
      421 atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtata tttatcttcc
      481 gcaaggccca gctcctcata cggctcgacg tttttacgca gcagcgcgcg tacggtaagc
      541 cccatatcgg caatgagttt gaccagttga tcgcgcgttg gcggtgtttc cagataatag
      601 ataatggtcg gttctgtacc gctgttgcgg atcatctcca gcgtattacg cgacgtgccg
      661 caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta caaagtgaaa
      721 gagagacgta gcgccagcgc agccagcgtt acaaaccaaa gcggataagt caaaaacata
      781 tatggtttaa cgaatgcgta tgtgcagagg aaggtaatag gtgtgaattt tgagttggct
      841 attcatttga aaggaggtcc gaatcaggac gtaaaaaaac cgactttgcg tcggtttttt
      901 actttccagc cctgagttgg tggctctggc tggagtgaga tagccatcat ctaacgcatc
      961 aatgctaata ccatactgaa actattgcaa ggacgtgctg gttttataac ctgcatgtac
     1021 tgtatgatta tacagttagc tctgaggcat tttcactctg gcaatgcgca taaacgcttt
     1081 caaagtcctg gtcagaagta cgggtggtgc cgttaactga tgctctggcc ggagtgagat
     1141 agtcgtcatc taacaatgag acatgcaccg tgacaggcag tggatgagta agcggatgca
     1201 ttctcactcc atcgcatgga gaaaacgggt gattgataaa gcaatcatcg ttcttggggc
     1261 gttaattgcg ctgctggaac tgatccgctt tctgcttcag cttctgaact gatagcggaa
     1321 acgtaattaa gggctaagag cacactactc ttagcccttt aacatttaac gcatggtcac
     1381 gaactcttct gccgccgttg ggtgaatggc gacggtattg tcgaagtctt ttttggttgc
     1441 ccccatcttc agcgccaccg cgaagccctg caacatttcg tccataccaa aaccaatgcc
     1501 gtgaataccg acaatcttct cttctggccc aacgcacacc agcttcatgc ggcacggctg
     1561 gcggtgagtg gtgacggcgg tatacatcgc ggtgaaagag gatttataca ctttcacctg
     1621 atcgtcgccg tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac caatcggcgg
     1681 atggctgaag accacggtcg gaatgttgct gtaatccaga tgctcatccg gcttgttatt
     1741 aaacaggcgt tcagagaggc ggcgacccgc tgcaactgcc accggtgtca gttctactgc
     1801 acccgtgtta tcgcccaccg cgtaaatacc ttcaatgttg gtgttttgat atttatcgac
     1861 gacgatatag cctttttcgt tggttttaac gccagcggct tcgaggttga tgttgtcatt
     1921 ggcaggctcg cgaccaatcg cccaaatcag gcaatccacc gtttcactgc gaccatcttc
     1981 cagctccagc gtcaggctac catcggcatt tttcactacc gctttcggga tggcgttggt
     2041 gtgcaactgc gggccttcgg cgttcatcac ttcgaccagc gtttcagaga tcatcgggtc
     2101 gaagctgcgc agcggcgcat gtttacgcac aaacagatgc gttttcgcgc ccaggccgtt
     2161 aatcacgcct gccagttcca cggcgatgta acccgcgcca acaaccgcca cgcgctctgg
     2221 caaagcagga agggcgaaga agccatcaga atcaataccg tattccacgc ccggaatatc
     2281 cgggtggctc ggacgaccgc cggtggcgat caggatatga tcggccgtga tggtttcgcc
     2341 gtttacttcc agcgttttgg catcaacgaa gcgggcaaag cctttgatta catcaacgtt
     2401 atttttaccg agcacgtttt cataggaagt atgaatgcgg tcgatatagg cggtacggct
     2461 ggcgatcaac gtttcccagt tgaatttatt gatagtggta tcaaaaccat aatccgggcc
     2521 gtacatatgg atcgcttcac ggatttgcgc cgcgtgccac atcactttct tcggtacgca
     2581 gccgacgttc acgcaggtgc cgcccagctc tttggcttca atcagtgcgc atttctggcc
     2641 gtacatagcc gcgcggttga tggaggcgat accgccgctg ccgccgccga tggcgatgta
     2701 atcatagtgt ttagtcatga cagagtgtcc ttatcgttga ttaccgcgat tgtagcgcga
     2761 gacgtaatag gtgccagcaa tggctgcaat tactccggca cgatccagct tacggtggcg
     2821 tgcccggtac ctgccggaac cagtttgctg tgcagccacg gcagcacgtt attcatctgc
     2881 tgttccagct tccacggcgg gttaatcaca atcatgccgg aagcggtcat gccacggcga
     2941 tcgctgtctg gcagcaccgc cagttcaatt tgcagaattt tgcgaatgcc agtcgcttcc
     3001 aggtcgtgga tcatgcgctt aatttgctgg cgcagcacca ccggatacca caatgcgtaa
     3061 gtgccagtgg cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc ctgatagtcg
     3121 gttttcattt catacggcgg gtcgataagg attaaaccac ggcgggaaac cggcggcagt
     3181 ttggccttaa gctgctggaa accgtcggct ttttcgactc gcgcacggct gtctttctga
     3241 aattcagaac gcagcaacgg gtaatcgctc gggtgcagtt cggtcagttg caggctgtct
     3301 tgttcgcgca gtagctggcg agcaatcaac ggcgaaccgg ggtagtaacg caactgacca
     3361 ctacggttga agtgttttac cacattgatg tacggctcca gctccgcagg caaatcgtcc
     3421 tgctgccaga tacgggcgat accttcgaga tattcgccgg tacgctcggc atgttcgcta
     3481 cctaactgat aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg tttatctttc
     3541 tctttcagcg actcgatgat caggctctga acggtatgtt taaggacgtc ggcgtggttg
     3601 ccagcgtgaa agctgtggcg ataactgagc atgggtaaag gtgttccggt aagtaaaaat
     3661 cggcccgtat tcggggcgca caaaagccta tcaggacagt ataccgaaaa gaggccgccg
     3721 ccgcgaaagc gtaacgtttc tcattgaaat tcactacact taaccccatg ctacaaacat
     3781 tatgtaaagc gcctgttgag cgcttcctta acctctttaa ctaggactgc gctaatgacg
     3841 aatccgttac tgactccctt tgaattgcct ccgttttcta aaattctccc ggaacatgtc
     3901 gttccagccg tgactcaggc gctgaacgac tgccgcgaaa atgtggagcg cgtagtagcg
     3961 caaggggcac cgtacacctg ggaaaatctc tgccagccgc tggcggaagt ggacgatgtg
     4021 ttagggcgta tcttctcccc ggtcagccac ctgaactcgg tgaaaaatag cccggaactg
     4081 cgtgaagcct acgagcaaac cctgccgctg ctgtcggaat acagcacctg ggtagggcaa
     4141 catgaagggc tgtataaagc gtatcgcgac ctgcgcgatg gcgatcatta cgccacgctg
     4201 aacacggcgc agaaaaaagc ggttgataac gcactgcgcg actttgaact ctctggcatc
     4261 ggtctgccga aagagaaaca gcagcgttac ggcgaaattg ctacccgtct ttctgaactg
     4321 ggcaatcagt acagcaacaa cgttctcgat gcgacgatgg gctggaccaa actcgttacc
     4381 gacgaagcgg agctggcggg gatgccagaa agcgcgctgg ctgcggcaaa agcccaggcc
     4441 gaagcgaaag agctggaagg ttatttactg acgctggata tcccaagcta cctgccggta
     4501 atgacctact gcgacaacca ggctctgcgt gaagagatgt atcgcgctta tagcactcgc
     4561 gcctccgatc aaggcccgaa cgccggtaaa tgggataaca gcaaggtgat ggaagagatc
     4621 ctcgctctgc gtcacgaact ggcgcaactg ctgggctttg aaaactacgc ctttaaatcc
     4681 cttgccacta aaatggcaga aaacccgcag caggtgcttg atttcttaac cgatctggca
     4741 aaacgcgcgc gtccgcaagg cgaaaaagag ctggcacaac tgcgcgcctt cgccaaagcc
     4801 gaatttggcg tggatgagtt gcagccgtgg gatatcgcgt actacagcga aaaacaaaaa
     4861 cagcacctct acagcatcag tgacgaacag ctgcgtccgt acttcccgga aaacaaagcg
     4921 gttaacggcc tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa agagcgtaaa
     4981 gatgttgatg tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga gaacaacgaa
     5041 ctgcgcggta gcttctacct cgacctgtat gcccgtgaaa acaagcgcgg cggggcgtgg
     5101 atggatgact gcgtaggcca gatgcgtaaa gccgacggtt cgctgcaaaa accggtcgcg
     5161 tatcttacct gtaacttcaa ccgcccggtg aatggtaaac cggcgctgtt cactcacgac
     5221 gaagtgatca ccctgttcca cgagttcggt cacggcctgc accatatgct gacccgcatc
     5281 gaaaccgccg gtgtatccgg gatcagtggg gtgccgtggg atgcggtcga actgccgagt
     5341 cagtttatgg aaaactggtg ctgggagcct gaggcgctgg cgtttatctc tggtcactac
     5401 gaaaccggcg aaccgctgcc gaaggagctg ctggataaaa tgctggcggc gaagaactac
     5461 caggcggcgc tgtttattct gcgccagctg gagttcggcc tgttcgattt ccgcctccat
     5521 gccgagttcc gcccggatca gggagcgaaa atcctcgaaa ctctggcaga aatcaagaaa
     5581 ctggttgccg tagtaccgtc tccatcctgg ggccgtttcc cgcacgcttt cagccatatt
     5641 ttcgccggtg gttatgcggc aggttactac agctacctgt gggccgacgt gctggcggca
     5701 gatgccttct cgcgctttga ggaagagggc attttcaacc gtgaaaccgg acagtcgttc
     5761 ctcgacaaca ttctgagccg tggcggttca gaagagccga tggatctgtt caaacgcttc
     5821 cgtgggcgtg aaccgcagct ggatgcgatg ctggagcatt acggcattaa gggctgatca
     5881 ttcagtgaaa atctgcttaa ttgatgaaac aggcaccgga gacggtgcct tatctgttct
     5941 ggcggcccgc tgggggctgg agcacgatga agacaacctg atggcgctgg tgttaacgcc
     6001 ggaacatctg gaattgcgca agcgtgatga gccaaaactt ggcggcatct ttgttgattt
     6061 tgttggcgga gcgatggcgc accgacgcaa attcggcggt ggtcgcggtg aggcggtggc
     6121 gaaagcggtg ggcattaaag gcgattattt gccggatgtg gtggacgcta ccgcagggct
     6181 ggggcgcgat gcctttgtgc tggcttcggt cggctgccgc gtgcggatgc tggagcgtaa
     6241 tccagtcgtt gccgcgctac tcgacgacgg cctggcgcgt ggatatgcgg atgcggaaat
     6301 cggcggctgg ttgcaggagc gtttgcagtt aattcacgcc tccagcctga cggcgctgac
     6361 cgatattacc ccgcgcccgc aggtggtcta tctcgacccg atgttcccgc ataagcagaa
     6421 aagcgcgctg gtgaaaaaag agatgcgcgt ctttcagtcg ctggtgggac cggatcttga
     6481 tgccgatgga ttactggagc ctgcacgctt gctggcgacc aaacgcgtgg tggtaaagcg
     6541 cccggactac gcgccaccgc tggcgaatgt cgccacgcca aacgcggtag tcactaaagg
     6601 gcataggttt gatatttatg ccggaacgcc agtgtaaaag taaagcaggg tagcgataac
     6661 gctaccctgc tttctgcaag aataattaat ggctttccgg cgtcgcaatc atgcgtttca
     6721 gccacggcac catcaacagc atcactactg caacgcccag cgtgaccaga ccaatcttac
     6781 caaacacgtt ggtatagacg ggcaacgtct caagcggatc ggtaatgttg tccggcaccg
     6841 cggtaaatgt tgccacatag ccgcccagca agaacgcggc agcctgcgtc aggaaccaca
     6901 tcccgagaat aaagcccatc aaatgctgcg gcaccagagc agcaatcatc gccaggccaa
     6961 gggcgctaat aaacagttca cctaagctct ggaataagta caccagcacg ataaaccatg
     7021 gcgatgtcag cccttgtgca tccgcaaacc acattcccgc agctgccgcc gtcaaaaagc
     7081 ccagtgagca cataaacatg ccgagagtaa atttcatcgg catcgagagg tctttgcctt
     7141 tgttacccaa atgcgtgtag atacctgcca gtattgggct ggcgagtacc acccagaacg
     7201 ggttaagcgc ctggaagctg accgggttga tggaaaagcc gagaatttca tgatgcacgt
     7261 tgttgatggc aaagaagttc agcgacgttg gcatctgggc gtagagaatg taaaacacca
     7321 ccgcttcgag catcaggaca aaggcaacaa acattttatt gcgcccggtt ttatccagct
     7381 tgaatgcctg acgaaagaag atgatggtga cgacgatgga gagaacaatc agcaccagat
     7441 tggcgacttc tacgttgtgc atcagccatg cacagacgaa gatcatcacc acgctgccaa
     7501 gtaacacgta caacagtttg ctgaagctca ttggccggaa gtcgggttca gaaccaatgt
     7561 ctttcaccat tccacgacag gcgatgtaaa ccagtaatgc gataattaac cccgccccgc
     7621 acaggttgta ggtgacagaa taaccgaatc tatcagcgat cacaggggcc agcgataacg
     7681 ctatcaacga gccgatattg atcgacatat agaacagggt gaatgcgcca tcaaggcgcg
     7741 gatctttcgg cggatagcac ttcgaaagca agctggctgg gttagcttta aacaggccgt
     7801 taccgacagc gatagtcccc agggcgatga aaatcaggtc aggcttaagt agcgacatgc
     7861 cggtcatgaa gtagccaatc gccagcacaa gtgctccaag aacgatggtg cgtttggtcc
     7921 ccagcaggtg gtcgccgaca tagccgccaa tggaaatgag gccatagacc agcgcagcaa
     7981 aagcaccaaa agtgacaaaa gcctgctctt gcgagaatcc aagctgttta acgaagaaaa
     8041 ccgccagtac gccctgcacg ccgtagtagc cgaatcgctc ccataactcg acaaaaaaga
     8101 tcatgaaaaa tgggcgaggt tgctgcagca tccccatggg tgttgttgta ttcatattta
     8161 ttaaccttcc aataccatcc tgaaaagtat gaacgcgtaa gggataacgc gcgaaaaagt
     8221 ttacatctgt cagagattct taatattgat gcggtgaata gattaataca ctattacaac
     8281 agaaaataac cagctgatca cactaatgat ggaaaatatg atggtgttaa atattgtatt
     8341 agctggggat tttgattgcg aacgcagacc gtaggccaga taaggcgttt acgctaatca
     8401 ggtaacaagg cgtgccaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg
     8461 tggagggaag attattcttc ttcgtcgcgc agcggaacaa tcagcatatc aacgtgaacg
     8521 gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg gtggtgacca
     8581 caaaccacta gatccatatc gtatttcttg attgcatcga ccagaacctg gcccaggtcg
     8641 ccgctgccgc tcagggtttc agtgattggg tagcctgcat tagtggaaag ctcggtcagc
     8701 gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac caagattcac atcaataagc
     8761 ccggtatata ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtat
     8821 gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga gagatcgacc
     8881 gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg
     8941 gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg
     9001 ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga
     9061 tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga atatattgat
     9121 agtggttaac cttctggaaa aaaaacaacc tgatctccta cactatctat agagccgctc
     9181 ggatgttggt taccacaaaa gcggtttctc caggacctgg tgttaactga ttggcagcgt
     9241 atcgggagcg gtagccccgg ggtggaactc cgcgggcagg tcgccgggga ggaggtatga
     9301 taagcaccgt cgcattattt tgggctttat gtgtcgtttg cattgttaac atggcgcgct
     9361 atttctcatc actacgcgcg ttgttagtgg tactgcgtaa ctgcgatcca ttgctctatc
     9421 aatatgttga tggagggggc ttttttacct cacatggcca acccaacaaa caggtgcgtc
     9481 tcgtttggta tatctatgcc caacgttatc gcgatcatca cgatgatgag tttattcgcc
     9541 gctgtgagcg ggtgcgtcgg cagtttattc tgaccagcgc attgtgtggt ctggtggtgg
     9601 tcagcctgat tgccttgatg atttggcatt aagcacttca gcaataataa cgcataaaaa
     9661 aagcgggcca gtaaactgac ccgctcgttt tggtgtgtac gattacagga actgcaagga
     9721 gagccagtac agcccgccgg aaagcagtac cgcagccgga agggtaaaca cccaggccat
     9781 cagaatgctg gtcacggttt tacgctgtaa gccgccacca tctaccacca tcgtccccgc
     9841 gacagaagag gagagtacgt gagtagtgga aaccggcatc ccggtataac tcgccaggcc
     9901 gatagacact gccgcagtca tctgggcaga catcccctga gcgtaggtca tgcctttctt
     9961 accgattttc tcaccgatag tcgttgccac acgacgccag ccaatcatcg taccgatacc
    10021 taacgccagc gcgaccgcca tgatgatcca caccggtgca tactcgatgg tgctaagcat
    10081 gtcggacttc agtttcttca acaggcgctg atcgtcagca ctcacgccag gcatcttcac
    10141 cactttgtcg atagtgtcag aaacgcacag cataatgcgg cgcatctggc tacgttgatc
    10201 aagcgacagc ttgtcgtagc tttccacatc ggtggtcaac atacctttca ggcggttgag
    10261 cgcgttaatg gtattcgacg gatggcagtg gaactccgca ggttgcgttg cgccagcttc
    10321 cggagccggt actaattgat cagcaccagt agcttgtttc agcagcgcag gatgctgctc
    10381 aaagtaagct tcgacgttgt tgatggcatc acgggtacgg gtgatttcgt agccagtggc
    10441 attcatgttc accacgaagc ctgctggtgc gacaccaatc aataccaaca taaccagacc
    10501 aatgcctttc tgaccatcgt tcgcgccgtg cgaaaacgcc acgccgatag cggaaaggat
    10561 cagcgcgata cgcgtccaga acggcggctt tttcttgccg tctttctttt cacgctccgc
    10621 tggggtcagg tggatacggg cgcgtttctt ggtgccgctc cagtagcgac gcagcaagaa
    10681 aatcagaccg ccagcaaaca ccaggccgac aataggggaa acgatcagag aaccgaaaat
    10741 acttaatact ttcgggatat tgagtgcatc caccactgac gtcccggtca tcaacgcatt
    10801 ggttaaacca atcccgatga tcgcgccaat cagcgtatga gagctggatg caggcaaacc
    10861 aaagtaccag gtacccaggt tccagataat cgccgccagc aacatagaga acaccatggc
    10921 aaggccatga gacgatccca tattaagcag cagatccgtc ggcagcatat gcacaatggc
    10981 ataggcaaca ctcagaccac ccagcaaaac acccaaaaag ttgaacaccg ctgccataac
    11041 cacggcgagc tgagaacgca tcgcgcgggt atagataacg gttgccacgg cgttggctgt
    11101 gtcatggaaa ccattgatgg cttcgtagaa cagcacaaaa gccagtgcaa gcaataataa
    11161 cagcccggta tgcaaatcca ggccagcaaa caaatgtagc ataggacgtt acgccatttt
    11221 gaggacatga acgcggcgca ttatcagtga ctttcgcggc gcgggcaaag tgaaatatag
    11281 actttttttg atttgcctcc tgtatggatt tcactcaaaa aataattatc ttatataatt
    11341 caggcaaata cttcctttta gtaatattga tgctggtgcg accactgagg aatctttaca
    11401 attcacgccc gttttttcta agaggagcgc aacgtggaaa ggtttgatgc cattattata
    11461 ggcgctggtg cggcgggtat gttctgttct gcgctggcag gtcaggcagg acgccgggtt
    11521 ctgctgatcg ataatggtaa aaaaccaggg cgcaaaatcc ttatgtctgg cggtgggcgc
    11581 tgcaacttta ccaaccttta tgtcgaacca ggcgcttatc tgagccagaa tccgcatttt
    11641 tgtaagtctg cactcgcacg ttttacccag tgggatttca ttgatctggt caataaacac
    11701 ggcatcgcct ggcacgagaa aacgttaggg caactcttct gcgatgactc cgctcagcag
    11761 attgtcgaca tgctggtgga tgagtgcgag aagggcaatg tgaccgtcag attgcgtagc
    11821 gaagtgctga gtgtggcgaa ggatgataca ggcttcacgc ttgaactgaa cggcatgact
    11881 gtcggttgcg aaaagctggt catcgcgacc ggtgggctgt caatgccggg gctgggcgca
    11941 tcgccgtttg gttataagat tgccgaacaa tttggcctca acgtgctgcc gacccgcgcg
    12001 ggtctggtgc cattcactct gcataaaccg ttgctcgaag agttacaggt gctggcgggc
    12061 gtggcggtgc cttccgtgat taccgctgaa aacggcaccg ttttccgtga gaacttactc
    12121 ttcacccatc gcggcttgtc tggaccggcg gtgttgcaaa tttccagcta ctggcaaccg
    12181 ggggaatttg tcagtatcaa tctgctaccg gatgtggacc tcgaaacctt cctgaatgag
    12241 cagcgtaacg cacatccgaa tcagagcctg aaaaacacac tggcggttca tctaccgaag
    12301 cggttggttg aacgcttaca gcaactcggg caaatcccgg atgtttcgct aaaacagctc
    12361 aacgtgcgtg accaacaggc actgattagc acattgaccg actggcgcgt acaacccaac
    12421 ggcactgaag gctatcgcac tgccgaagtg acgctcggcg gcgtggacac caacgaactc
    12481 tcttcacgga cgatggaagc gcgcaaagtg cctgggctgt acttcatcgg cgaagtgatg
    12541 gacgtcaccg gctggctggg gggctataac ttccagtggg cgtggtcgag tgcatgggct
    12601 tgtgcgcagg atttgattgc agcgaagtcg tcctgagctt tttcctaaag atgaaaatta
    12661 ttagagtcgc attaaaaatg agctaatttt gatagtggct atcttgtgat tattttctaa
    12721 tgagcccgtg aactgaaccc cccatgctta atataaggtg gagggaaagg tgattgaaaa
    12781 ctcacccagt gggctgatct ttataaaata tgaacaggtt gaaaaaaaac agtaactttg
    12841 ctgtttttta tacttaacca ctatgcatta atgctgcgtt atttcattct acctaagaaa
    12901 aaccagcgtt acgcaaatgg tcaacgctgg ttttatccgg tacgttgcaa ttatttttta
    12961 gcagaacccg cttctaatat tgaaacgatt gagaacaacg taaagcatac cgctcccaaa
    13021 ccgaccagaa cccgcgaagg aataaaatat cccatatcgg tctgcgccat ttccgcaaga
    13081 aacgatgcca ggaaaaggca aaacaggcag gtgaagacgg gaatcatcgg tattctgttg
    13141 gctaacgaac aggtacgtct ccagaccagt gccagtagcc agacttttga gaatatgctg
    13201 taacagatca ttccgaggca aatgagaata atgccgggag ccagtcgggc gcttgcatcg
    13261 gagctgacta agacgtatat gccctgcagc accgtgattg agccgagaaa aataacccaa
    13321 taacaccaca gccagtgctc tttcgttgaa aacgtattac gtgtttgatg gacgattgtg
    13381 gcaactaggc caattagaca ggcacagatt gcggttaacc cgagcaatac gtgaccggcg
    13441 acataatgcg gagttatgtc ggcactacgt aacagcgtaa tagaccaaat gaaaccgagc
    13501 agggtgagta acacgggaac ggcaatgagg caattaccga ttaatgaaga ataagcctgt
    13561 accggtgttt cgtcgctctt gctccccgct gcgtttttag gaatgagcag aaagtgaccg
    13621 gatgacgctg ccaccgtcga tacacaggcg gcaatcatac caacgccgaa aataacatgg
    13681 ccggcgacaa actcgtctgc catcacatca ttacctgcta acagtgccca accccatatc
    13741 atggtgataa ttgaccccgc atagccaata atggggaaca atgtattgta aaacgtatta
    13801 acgccgcgcg tgagctgcga aatgataata aatgcggtag tgaataatgc caggcatatg
    13861 gctgccagag aaatgagcac atgacccgcg acgaaatatt cgctttgccc tgaactttcc
    13921 agcacaaagc cacctaacgc gatacaaatc aagcccataa ttaaagggat taatttgaaa
    13981 agccacccga tatatatgtt cactttaaac tcctcaggta tccctgctaa tattgaaatg
    14041 tgcattaatg ataatttcag cctgttaata caatgacgag atatcattaa agacaaatgc
    14101 atagcataac tatagcggag tttactttgc agagcgctac ttttcagagt tcaaaaggcc
    14161 atgataaata aatatttatc ctaagattaa ttcttattct gttgtttttt acaattgtct
    14221 tccagggagg gattcttttt cattatttgg taaaagaccg gatggtctcg gtgcttaaaa
    14281 tcagcgagca tatacgcatt attgctttat atcgtcaaaa aatatgtctt tcagacatgt
    14341 atcatttatt ttgttactaa caagtcaaaa atatcacggg ttatttatga aagtatttca
    14401 caatatattt aagtacatct cttcaaacca tcaagataag cattcagata aagttaataa
    14461 tcatcaacac catggcaagg ttgataaaaa acatcgttca gagatagttg aaattgataa
    14521 gctggataaa cattcgcaaa tagataacga ttttggcctg catatcatct atttcctgca
    14581 acatggctgc tggaacgtaa ctgaacatag tcctcagata gagaaaatat ggttttacaa
    14641 tag
//
LOCUS       DAZFFG010000111        14554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFFG010000111 DAZFFG010000000
VERSION     DAZFFG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.111"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            430..1701
                     /locus_tag="ACHJ7J_003866"
     CDS             430..1701
                     /locus_tag="ACHJ7J_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO5002043.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SKLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1731..2735)
                     /gene="dppF"
                     /locus_tag="ACHJ7J_003867"
     CDS             complement(1731..2735)
                     /gene="dppF"
                     /locus_tag="ACHJ7J_003867"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO5002044.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2732..3715)
                     /gene="dppD"
                     /locus_tag="ACHJ7J_003868"
     CDS             complement(2732..3715)
                     /gene="dppD"
                     /locus_tag="ACHJ7J_003868"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO5002045.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3726..4628)
                     /gene="dppC"
                     /locus_tag="ACHJ7J_003869"
     CDS             complement(3726..4628)
                     /gene="dppC"
                     /locus_tag="ACHJ7J_003869"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO5002046.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4638..5657)
                     /gene="dppB"
                     /locus_tag="ACHJ7J_003870"
     CDS             complement(4638..5657)
                     /gene="dppB"
                     /locus_tag="ACHJ7J_003870"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO5002047.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5808..7415)
                     /gene="dppA"
                     /locus_tag="ACHJ7J_003871"
     CDS             complement(5808..7415)
                     /gene="dppA"
                     /locus_tag="ACHJ7J_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO5002048.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(8168..8244)
                     /locus_tag="ACHJ7J_003872"
     tRNA            complement(8168..8244)
                     /locus_tag="ACHJ7J_003872"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8208..8210),aa:Pro,seq:cgg)
     gene            complement(8336..10027)
                     /gene="eptB"
                     /locus_tag="ACHJ7J_003873"
     CDS             complement(8336..10027)
                     /gene="eptB"
                     /locus_tag="ACHJ7J_003873"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO5002049.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWCILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            complement(10282..10812)
                     /gene="lpfE"
                     /locus_tag="ACHJ7J_003874"
     CDS             complement(10282..10812)
                     /gene="lpfE"
                     /locus_tag="ACHJ7J_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO5002050.1"
                     /translation="MKFNLLLHSGIASLSLFACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            complement(10817..11878)
                     /locus_tag="ACHJ7J_003875"
     CDS             complement(10817..11878)
                     /locus_tag="ACHJ7J_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002051.1"
                     /translation="MKAAIALSLLGCVFGFSGKALAGGSWGPCTPVDGTTYHYNVDVD
                     VGVPDASKNVAGTVLTDVLNWSNGQNVSIICECPDKGDYKIEKDTLIQGVSGIANMTR
                     DIGGFKYYYLTDELSVSTKIQVSSGQYGFVPFKGQQALQVTGCNEVITTPYMGGGGLL
                     SFLITKPFIGDSVIPPTLIAELYASKTNKDYGTMPISSVSIQGRVTITQDCEIKPGTV
                     LDVPFGEFPSSAFKNRQGQMPEGATEQEINLGFDCNNISDGIKVSLRLEGATNADDPR
                     AVDMGNPDIGVLVKDSSGKILVPNDASSTTLLNLSSLDSKTHRNAAIRLLALPISTTG
                     KAPKGGTFEGVTTIYLEME"
     gene            complement(11893..14466)
                     /gene="lpfC"
                     /locus_tag="ACHJ7J_003876"
     CDS             complement(11893..14466)
                     /gene="lpfC"
                     /locus_tag="ACHJ7J_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO5002052.1"
                     /translation="MSRKTVSRTFSSFSISVVAVAVASTFSAHAGKFNPKFLEDVQGV
                     GQHVDLTMFEKGQEQQLPGIYRVSVYVNEQRMETRTLEFKEATEAQRKAMGESLVPCL
                     SRTQLAEMGVRVESFPALNLVPAEACVPFDEIIPLASSHFDFSEQKLVLSFPQAAMHQ
                     VARGTVPESLWDEGIPALLLDYSFSGSNSEYDSTGSSSSYVDDNGTVHHDDGKDTLKS
                     DSYYLNLRSGLNLGAWRLRNYSTWSHSGGKAQWDNIGTSLSRAIIPFKAQLTMGDTAT
                     AGDIFDSVQMRGAMLASDEEMLPDSQRGFAPIVRGIAKSNAEVSIEQNGYVIYRTYVQ
                     PGAFEINDLYPTANSGDLTVIIKEADGSEQRFIQPFSSVPIFQREGHLKYSFAAGEYQ
                     AGNYDSASPRFGQLDLIYGLPWGMTAYGGVLISNNYNAFALGIGKNFGYIGAISIDVT
                     QAKSELNNDRDSQGQSYRFLYSKSFESGTDFRLAGYRYSTSGFYTFQEATDVRSDADS
                     DYNRYHKRSEIQGNLTQQLGAYGSVYLNLTQQDYWNDAGKQNTVSAGYNGRIGKVSYS
                     IAYSWNKSPEWDESDRLWSFNISVPLGRAWSNYRVTTDQDGRTNQQVGVSGTLLEDRN
                     LSYSVQEGYASNGVGNSGNANVGYQGGSGNVNVGYSYGKDYRQLNYSVRGGVIVHSEG
                     VTLSQPLGETMTLISVPGARNARVVNNGGVQVDWMGNAIVPYAMPYRENEISLRSDSL
                     GDDVDVENAFQKVVPTRGAIVRARFDTRVGYRVLMTLLRSAGSPVPFGATATLITDKQ
                     NEVSSIVGEEGQLYISGMPEEGRVLIKWGNDASQQCVAPYKLSLELKQGGIVPVSANC
                     Q"
BASE COUNT         3491 a         3919 c         3562 g         3582 t
ORIGIN      
        1 tggtctaggt tcaagattag cccccgtggt gttgtcaggt gcatacctgc aacgtgcggg
       61 ggttttctct ctccagcaac caatgccacc agggattaag cccccgcaac attgcgcctc
      121 accggaaccc ttccggcttg ccgctgattt tacgattgct ttttactgtc agcacccggc
      181 aatcttcctc attgcgcgat gccttccagc ttaatttctt tatttgcaat agttgcagtt
      241 catatccggg ctgactccag actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc
      301 tcgcgacctc cccagtgaat ctgttgcctc attttttaca tactgcgtat tcgacttctc
      361 cacctgttgc gcaagagaaa ctgggtttat tcatttttgc gaggccgact tctttctgga
      421 caggacttta tgcagcacaa cacactaccg aaacacgatc agaaattgcc gtttacacgc
      481 tacgacttcg gctgggtttt attatgcata ggcatggcga ttggtgccgg aaccgtgctg
      541 atgccagtac aaattggctt gaagggaatt tgggtattta ttaccgcagc gatcattgct
      601 tatcctgcca cctgggtagt gcaggacatt tatttaaaaa ccctttctga aagcgattcc
      661 tgtaatgact acaccgatat tatcagtcat tacctgggga agaactgggg aattttcctc
      721 ggggttatct actttttgat gattatccac gggattttta tctactctct ctccgtggtt
      781 ttcgacagcg cctcgtacct gaaaaccttc ggtttaaccg atgccgatct ttcacaatct
      841 ctactttata aagtcgctat tttcgccgta ctggtagcga ttgcgtctgg tggtgaacga
      901 ttactgttta agatttctgg gccaatggtg gtggtcaaag tagggattat tgtcgtgttc
      961 ggttttgcga tgatcccgca ctggaatttc gccaatataa ccgccttccc gcaagcctcc
     1021 gtcttttttc gcgatgtcct gcttaccatt ccattttgct tcttttctgc agtatttatt
     1081 caggtactta acccaatgaa tattgcctat cgtaaacggg aagcggataa agtactggca
     1141 acccggctcg cgctgcgtac ccaccgaatt agttatatca cgctcatcgc ggtgatactg
     1201 ttttttgcct tttcgtttac cttctcaatt agccacgaag aagccgtttc tgcctttgaa
     1261 caaaatatct cggcactggc actggccgcg caggtgatcc ctgggcatat cattcatatc
     1321 acctctacgg tgctgaatat ctttgccgta ctgaccgcat tctttggcat ttatctcggt
     1381 ttccacgagg ccattaaagg cattattctc aatctgttaa gccgaattat tgataccaag
     1441 aaaattaact cacgcgtgct gactctggcg atctgcactt ttatcgtcat tacgttgacg
     1501 atttgggttt cgtttcgtgt atcggtgctg gtgttctttc agttgggaag cccgttatat
     1561 ggcattgtgt cgtgcctcat tccgtttttc ctgatctata aagtctcaaa actggaaaaa
     1621 cttcgcggat ttaaagcctg gctgattctg ctttacggca ttttgctatg cttgtcgcca
     1681 ctgttgaagc tgattgagta aaccggagcg catggccccg gttttgtgag ttaacgctgc
     1741 ggattttcat cctgatcaac agcaaaacaa gctacaagtt gaccgccgta gtcttttagc
     1801 tgcggctgca actgggtgca ggggccgaag cgccgacgac agcgggcgtt aaacgcgcag
     1861 cccggcggtg gattcagcgg gcttggcagt tcaccggtga gtttgatgcg ttcgcggcga
     1921 tcgtccggat tcaggcgcgg cgtcgcggaa agtagcgcct gagtgtacgg atggcgcggg
     1981 ttattgaaga tttggtcctt cgttcccttc tccacgcagc ggcccaggta catcaccatc
     2041 acttcatcag caatgtgctc caccaccgac agatcgtggg agatgaagac ataagaaagc
     2101 cccagttcct gctgcaaatc catcatcagg ttgagcacct gtgcacgcac cgagacgtcc
     2161 agcgcggaga ccggttcatc ggcaatcacc acgtccgggt cgagcatcag accacgggca
     2221 atagcgatac gctgacgctg accgccggag aacatatgcg gatagcggtc atagtgctcg
     2281 gttttcaggc cgactttcgc catcatcgac agggcttttt cccgacgctg ttctttgctt
     2341 aagctggtgt tgatcagcag cggctcttca agaatttgcc cgactttttt acgcggattc
     2401 agcgaaccgt aagggttctg gaagacaatc tggattttct gccgacgcag cttctgcgcc
     2461 tgcggatcgt gcttaagcag atcctgcccc tggtaataca gctcgccgcc ggtgggagtt
     2521 tcaatcatcg tcagcaaccg accgagggtc gatttaccgc aaccagattc acctactacc
     2581 gccagcgttt tgccacgttc aaggttaaac gaaacgccgt ccagcgcctt caccagacgt
     2641 tccggagcga acataccttt cttcaccgga taatgttttt tcaggtcgat agcctgcaac
     2701 agcggttgtt gcgaggtggc ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa
     2761 gtgggtaatg gcatttggac tgacgcccgt cagcgagcat attcagcgcc ggttcttcag
     2821 cgcgacatct gtcagtggca taggggcagc gcgggttaag caggcagccg ttcgggcggt
     2881 cgtacttgcc gggaacaacg ccaggcaacg atgccagacg ttctttgtcc tgagcaaatt
     2941 ccggcagcgc acgcagcaat gcctgagtat acgggtgacg cggcgcatgg aagatggcgt
     3001 gcgcatcgcc cgtttccacc acctgaccgg catacatcac gatgatttta tgtgccgctt
     3061 ccgccaccag cgccaggtca tgggtaatta acaccagcgc catgttctct ttctgctgta
     3121 gctccagcag tagttcaatg atttgcgcct gaatggtcac gtccagcgca gtggttggtt
     3181 catcggcaat cagcagtttt ggccgacagg caatggccat cgcaatcatc acgcgctggc
     3241 tcatgccgcc ggaaagctga tgcgggtaaa catccagacg cgaagccgga tcgggaatac
     3301 cgacctgatt caacaagtca atcgcccgct gacgacgagt gctcttatta ccgccctgat
     3361 gcaccttaat cgcttccatg atctggaaac ccactgtgta gcacgggtta aggctggtca
     3421 tcgggtcctg gaagatcatc gccacttcgg cacccaccag gttgcggcgc tctttttctg
     3481 agatacgctg taaatcctga ccgttaaact ccagcttttc cgccattacg cggcccggat
     3541 aatcaatcag ccccataatc gccagcgaac tgaccgattt gccagagccg gactcaccca
     3601 caatcccgac cacttcgccc tgttttacgc tgtagctgat gcggtctacg gcgcggaacg
     3661 gtgcgctttc gtcgccgaaa tgcaccgata atttatctac atttaataac gccatctcga
     3721 acctcttact gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc catcaggtta
     3781 aatgccagca ccgtcagcag gatcgccaga cccgggaagg tcacgaccca ccaggcgctt
     3841 tgcgcgaact gcaacacgtc ggagagcatg gtgccccact ccggcgttgg cggctgcgcc
     3901 cccatgccga ggaaaccaag agcagccata tcgagaatgg cgttagagaa accgagcgac
     3961 gcctgaacaa tcagaggcgc aaggcagttc gggaagatgt taataaacat ctgacgcatc
     4021 gccccggcac ctgccacgcg agacgcggta acgtaatcgc gattcacctc caccagcacg
     4081 gcggcgcgtg ttaagcgcac atagtgcggc aaggcaacga aggtcagcgc cagcgcggcg
     4141 ttaccaatcg acgggccgaa aatcgccacc agcaccagtg ccaacagcag acttggcagc
     4201 gccagcatga tatcgaccac gcgcatgatg atgttatcga ccaggccgcc gaagtaaccg
     4261 gcgatcaggc cgagaataac gcccataatc agcgataaca caactaccag acagccaacc
     4321 agcagtgaca atcgcgcacc gtacatcaga cgcgacagca catcacggcc tacgtcatcg
     4381 gtgcccagca agtgcgccat gctgccgcct tcctgccagg ctggcggggc gagcagtgca
     4441 tcgcggaact gttccgccgg gttatagggt gcaatccagt tggcaaagat cgcgataaac
     4501 agcacgatga cgacgtaaac cagcccgacg accgcgcctt tgttgcgttt aaaatagtgc
     4561 cagaactcct gtaacggggt catcggtacc ggtgcgctaa tcactttatt ttcagtaacc
     4621 tgtgacatga tgtcccctta cttcttatga cgaatacgcg ggttcaccac gccgtacagc
     4681 agatcgacca gcaggttgac gaggataatc atcgtcgcca ccagcaatac gccgccctgc
     4741 actaccggat agtcgcggcg ttgcagtgcg tcaatcaacc agcgccccag accgggccac
     4801 gagaagatgg tttcggtcag aatcgccccc gccagcaatg ttcccacctg gaggccgata
     4861 acggtcacta ccggcagcat cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg
     4921 gtcaaccctt tggcgcgcgc ggtgcggatg taatcttcac ccagcacttc cagcatcgag
     4981 gagcgcgtca tacgcacaat gaccgccagc gggatggtac ccagcacaat ggcgggcaag
     5041 atcatatggg cgacagcatc gataaagttg ccgtcttcac cccagatggc ggtgtcgatc
     5101 agcataaaac cggttaacgg attggaatca tcgaggaata ccatgtcact cacacgaccg
     5161 gagacgggcg tcaggttcca gtgcaccgaa accagcatga tcagcatcat gccccaccag
     5221 aagataggca ttgaataacc tgtcagcgcc aggccaaccg ctgtgtgatc gaaaatggaa
     5281 ccgcgtttaa ccgcagccag cacaccaacc ggaataccga ctgccgtagc aaaaatcatc
     5341 gcgcagacgc caagttccag cgtggcctgg aagcgcggca cgaactcttc ccaaaccggg
     5401 atgcggcttt tcattgaaat gcctagatcg ccatgcataa cgccccaaat gtaatggaga
     5461 tactgctgcc acatcggttt atctaagccg agttcagcca gcagctgcgc gtgacgctca
     5521 ggggagatcc cacgttcgcc cgccatgatc atcaccggat cgcccgggat catgtggaca
     5581 aaggcaaatg tgagaagggt aataccgata aacgtgggga tgacgagtcc caaacgtcgg
     5641 agaataaact gcaacataac ccggattctc tgtagtgacc cgcagcctgg aactgcgtgt
     5701 ctgtattgct cacaaatcaa ttcccgccca ggtcgggcag ggaatgaagt attgctgccg
     5761 gatggcgctg cacgcagagg cacagcacca ccgggtatgg cttttaatta ttcgatagag
     5821 acgttttcga agtgatgttt gcctaatgga tcaaccacat agcctttaac ttctttgcgt
     5881 accggttcaa acacggtgga gtgagcgatg atcagtgccg gagcctgatc gtgcatcacc
     5941 acctgcgctt gcttgtacag ttcaacgcgt ttgttatggt cgtcggtagc acgcgccggt
     6001 tgaatcagat cttcaaacgg tttgtagcac cattttgagt agttggagcc ttgttcagag
     6061 gcggcgcagc tgaacagagt ggcgaagaag ttatccggat ccccgttatc gccagtccag
     6121 cccatcatta ccgtctggtg ctcgccatct ttcgcacgct tgaggtactc accccattcg
     6181 taggtgacaa ttttggcctg cacgcccact ttcgcccagt ctgcctgaat catctccgcc
     6241 atgcggcgag cgttcgggtt atacggacgt tgtaccggca tcgcccacag gtcgatggag
     6301 aaaccttttt ccagacccgc ttctttcagc aaggctttcg ctttttcagg atcgtaggtg
     6361 tagtcctgaa cgtcgtcgtt atagccccac atggttggcg ggatcaggtt tttcgctgat
     6421 acacccgcgc cctgataaac cgctttgatg atcgcgtctt tgttcaccgc gtaggtcaga
     6481 gcctggcgaa ctttcacgtc atcgagtggt tttttctgca cgttatacga gagataaccg
     6541 acgttcagcc ccggcatttc catcaggttg atagatttat cctgcttcat gcgagcgata
     6601 tctgccgggt tcgggtacgg catcacctgg cattcattct tctgcaattt cgcgtaacgc
     6661 acggaagcat caggggtaat agagaaaacc agcgtatcga tctgcggttt ggtgccccag
     6721 tagccatcaa acgctttgta gcggatacgg gaatcttttt ggtactgctg taactggaac
     6781 ggaccggtgc cgattgggtt gaggtccagt ttttccggtg taccggcttt catcatcgcg
     6841 tcagcatatt cttttgacag aatagaggcg aagtccattg ccaggtcagc gaggaacggc
     6901 gcttccggtc gagtcagcac gaactgaact gtgttgtcgt ccaccttttt cacttcgctg
     6961 atcagctccg gcaagcccat gccttcgaag tattcgtagc taccgccaga aactttatgg
     7021 tacgggtttt gcgcgttttt ctgacgatcg aacgagaaca ccacgtcatc ggcgttcagt
     7081 tcgcgcgtcg gtttgaattc tttattgtcg tgccacttca cacctttacg cagatggaag
     7141 gtgtaggttt taccgtcttc gctgacttcc cacttttcag cgaggcccgg gatcacttcg
     7201 gtggtgccga ttttaaattc aaccagacgg ttataaagcg ggacggaaga ggcgtcatag
     7261 gtggtgccgg aggtaaacag ctgcgggtta aacccttccg gagatccttc tgagcaataa
     7321 accagagttt tagcctgaac acttgctgcg acggtcatag ccaccaggct gagaccaagc
     7381 ttcagcatcc ctgacttttt caaggaaata cgcattattc tgctccaatt gtgatgtttg
     7441 ttgttttaac cctttgcagt gggttgtcgc tgcctgacct tttttgtttt ttgcccggtc
     7501 gggtcaacgt tatgaggtgg ggatgccgta ctaattaaca tcagtgtaac agtgcggatc
     7561 ctccataaaa tgcccctcgt gacctacaat ctgtcaacag aatgtgaaaa cgtcaataca
     7621 gccaaccggg atttacacca acggtgagaa tccacaaaca aagattaaaa aaacttcaaa
     7681 cagctatttg cagcagagaa atttgtgcta ctccaacatg accagaacaa tcagcttaat
     7741 atttagcaac atttggtgat taagttttat gcatttaatg aaaaaaatct gaggaaaagg
     7801 tggatatctg agcgattaat accatgaacg ctaaaacgca cagcggaaaa tgccagcgcc
     7861 agccataagt aacgcaaaaa aagatttaag caaatataaa aaaagacaat ggtttctgtc
     7921 acaaatccgt taatggatag tgagatatgg ggcgcaaatt tggcagggaa atgtgtggtg
     7981 tgctgccgtt taggtcggat aagacgggta cgctgcatcc gacaatgagt gcggtgccgt
     8041 tgcctgatgc gacgctgacg cgtcttatca ggcctacgtc gagtcgaacc tccctgcccc
     8101 cggaggttct catcctcttc aggtcgcaga aagcaaaaaa ccagcccgta ggctggtttt
     8161 tctaaattgg tcggtgatag aggattcgaa cctccgaccc cttcgtcccg aacgaaatgc
     8221 gctaccaggc tgcgccaatc accgaatgcg gggcgcatct tactgcgcag atacgccctc
     8281 gtcaatccct taatagcaaa atgccttttg atcggcgaga aagtcagcag gcctcttagt
     8341 tagccgctgc ctcttttgtc tgcgggatgt gacaccagtt gttgttttcg ttaattccac
     8401 catccggtga agtatagcca agacaaccca tgatggtgtc gtacagctct acgtgacggc
     8461 gtggcacttt catgtcggct gcttttttca gctgcgcaaa cgcctgcgca ttgaccggat
     8521 tttccagata tttatctgac atccagacca tcatcggtac gcggaactgc tccggcggtg
     8581 ccagttcacg cggcgtgccg tgcaggtgct cgcgttcatt aattgactcg ccgtggtcag
     8641 ctgcgtagaa cacaatcgct ttcttatcgc gaacctgatc aatcacactg gagataaagt
     8701 gatccacata ggtcaccgag ttgtcatagg agttgatcat ctgcgctttg gtacatccgc
     8761 tatcaacacc aatacattcc ggcttccact gcgcaaagct gcgcgggtaa cgctgggtgt
     8821 agttaaagtg cgaacctttg gtatgcagaa tgattagatg cttaccatcc gggttgcgcc
     8881 ccagcgattg ctgcatttcg tctaccagca acatatcatc taccggcttg ccacggttac
     8941 gtggctccgc accaatctgt tcacggtaag caatattgtc cgccatcgta ttgctgtaga
     9001 accacatttc gctctgcata gcgtagaggt cagaactgaa tcctaactgc ttcagtaccg
     9061 cgaaaatgtt ctgttctttt aatgtgcgct gcggattatc ttccgcgccc ccctgacgta
     9121 caaacatgca acgcagagag agtttggttg cggtatcaca ggagtaacca cggaacgccg
     9181 ccagattttt ctcctgggcc agcttcggcg tggtattacg ctcatagccg aaaatgccca
     9241 tatggtccca acgcgtagtt tcaccgatga taaacacgac ataagtgtca ttaacatttt
     9301 gcggtgcctg ataagtgaat ttcttcgccg ggttaagcaa tgaattatta tcggaagatt
     9361 catcgacccg cgcccaggcg tacagcccca acgcagaaag ccagtttgat ggcagataag
     9421 agttcgctac gacaccgcca taactcggca aatcaacgcc ggtcgccctc tccactttct
     9481 tctgctggat atccagcaaa cgaatcggtg cccaaaccat aattcccgcc agtacgacga
     9541 ccgccaggct gcgaatacgc tgccctgggg ttcgcagttg tcggagcaag gtgtagcgac
     9601 agcggttatt ccagataagg ataagaggca atgcgctaac ggcgattaac cagagaataa
     9661 agttcagacc aacaacttct tttgacaggt cgatatcggt ggtcatgacg gaagcgatga
     9721 tgccataacc aatgaccaca ttaaggaagg tcatgtaata gctggcacct gcggaaaaga
     9781 gcacaaccag cgatgccaga atacaccagc tgcggcggcc aaacagcgaa agaagacgta
     9841 gtaaaaagaa ggtcaccagt acggtggcgg ccagttcaac aacagcagaa atgcctttcc
     9901 agacggtaaa atcgtgcgca tagctgccga agcggcggta aaaaaccgcg ccattcataa
     9961 aaaggccaat atagattgca agcaaaaagc tcagcttctg ctgtgtaatc gatttgatgt
    10021 atctcatgca aacaaaccct ggacaaacag gtgataacac cctgcttagc ggcttttcag
    10081 gcccagcagg ggaaaagtgc ggaggtagtc agataagtaa aatctacacg cgcgaataag
    10141 tagaccacag caaagggaaa aagtgtgggg aaagagtgtg catgaagcac aaatttacaa
    10201 aagtgcaagt ccggaataga ccattttaac ggattaacga ctattcagag ataggttggg
    10261 ccaggatgct ggcccagtaa cttactcata ggtgatggaa aaattgacaa gggcattcgc
    10321 tgtgccacca gtggcctgag tgttgacgac cacataactc gcctggtagt gaagcgacgt
    10381 ggtttcgcca tcaatcgccc attcaatcgg cgttccgctg gagcctttgg gaacttgctg
    10441 cgttccttta tcgtcctcta tcttcaacgc aacacccgtt gcgccaccag cctcaagggc
    10501 aaacatggaa tcatcatcgc tgtccgactg actgtccatc tcaacggtcg ctttggatat
    10561 cccatcgcac tctttgagtt caatagtaaa atctttcttg ctcaacaccg taccgactgc
    10621 cgcaggtttt tgcagcgtca ttgttcccat atcgacttcg agatcgcttt tttccatctc
    10681 gcaagcttta gtggtgatag tgatggagaa atgaatatcc cctgccgggc caagatccgt
    10741 cgccgcattc accccacagg caaacagact caaactggcg atgccgctat gcagcaatag
    10801 attgaatttc atggcatcac tccatttcaa gatatatggt cgttacgcct tcaaacgtcc
    10861 cgcctttagg cgctttaccc gtcgtgctaa tcggtaaagc cagcagtcga atcgcggcat
    10921 tgcggtgagt tttagaatcc aggctgctca aatttaataa cgtggtgctg cttgcatcat
    10981 tcggtaccaa aattttgccg ctggaatctt tcaccagtac gccaatatcg gggttgccca
    11041 tatccaccgc tcggggatcg tcagcattgg ttgccccctc aaggcgcaac gagactttga
    11101 taccatccga gatattgttg caatcaaagc cgaggttaat ttcttgctct gtcgcacctt
    11161 caggcatttg cccttgtctg tttttaaacg ctgaggaggg gaattcacca aatggcacat
    11221 ccagcaccgt gccaggttta atttcgcaat cctgggttat ggtgacgcgg ccctgaatag
    11281 atacggaaga tattggcatt gttccgtaat ctttatttgt tttggaggca tacagttcgg
    11341 caattaacgt gggagggatg actgaatcgc cgataaaggg ttttgtaatc aagaaagaaa
    11401 gtaatcctcc tccgcccata tagggagtag tgatgacttc attacagccc gttacctgaa
    11461 gcgcctgttg acctttaaag gggacaaaac catattgccc cgaacttacc tgaatctttg
    11521 tggatacaga cagttcatcg gttaagtaat aatacttaaa accaccgata tccctggtca
    11581 tattcgctat tccactaacc ccttgtatca acgtatcttt ttcaatttta tagtcaccct
    11641 tatcaggaca ttcacaaatt atcgatacat tttgtccatt cgaccagtta aggacatccg
    11701 ttaaaaccgt ccctgcgacg tttttacttg catcgggaac accgacatcc acatcgacgt
    11761 tatagtgata ggtcgtgcca tccacagggg tacatggccc ccaggaacct ccggccaatg
    11821 ccttgccaga aaatccgaat acgcatccta ataaagataa tgctattgcc gctttcattt
    11881 cgctctcctt gcttactggc aattggctga aacaggaaca attccgccct gttttaattc
    11941 cagggataat ttatatggag ccacgcattg ctgcgacgcg tcattacccc atttaatcag
    12001 tacccgccct tcctctggca tcccgctaat atagagctgc ccttcctcac cgacgatact
    12061 gctcacctcg ttttgtttat cggtgattag cgttgccgtt gctccaaagg gcaccgggct
    12121 gcccgcggaa cgaagcagcg tcattaatac gcggtaacca acgcgggtat caaaacgcgc
    12181 tctgacaatc gctccacgcg ttggcaccac tttctggaac gcattttcaa cgtcaacatc
    12241 gtcacccaac gaatcgctac gcagtgagat ttcgttttca cgatacggca tggcgtaagg
    12301 cacgatcgcg ttacccatcc agtcaacctg aacgccgccg ttattcacca cgcgggcatt
    12361 gcgcgcaccg ggtacggaga tgagcgtcat ggtttcgcct agcggttggg aaagcgtcac
    12421 gccttcgcta tgaactatca cgccgccgcg aacgctgtag ttgagctgcc ggtaatcttt
    12481 cccgtagcta tagcctacgt tgacattacc ggacccaccc tgatagccaa cgttagcgtt
    12541 accgctgtta cccacaccgt tgctggcgta gccttcctgg acactgtagc tcaggttgcg
    12601 atcctcaagc agcgttccgc tgaccccaac ctgttgattg gtacgaccat cctggtcggt
    12661 cgtgacgcga tagttactcc aggcccggcc tagtggaacg gaaatattga aagaccacaa
    12721 gcgatcgctt tcatcccatt cagggctttt attccagcta tatgcaatac tgtaactgac
    12781 cttgccaata cgtccgttgt aacccgccga taccgtgttc tgtttacctg cgtcgttcca
    12841 gtaatcctgc tgcgttaaat ttaaataaac agagccatag gcccctaatt gctgcgtcag
    12901 gttaccctgt atttcgctgc gcttgtgata acggttatag tcgctgtcag cgtcactgcg
    12961 cacatcggtg gcttcctgga aggtatagaa accgctggta gagtaccgat agcccgcaag
    13021 gcggaaatcg gtgccgcttt cgaagctctt ggaatataag aatcgataag attgtccctg
    13081 gctatcgcga tcgttattca gttcgctttt agcctgcgtc acatcaatgg aaatcgcccc
    13141 gatataacca aagtttttcc ctatccctaa tgcaaatgca ttgtaattat tagagattaa
    13201 tacgccgccg taggccgtca tcccccacgg taaaccgtag atcagatcaa gctgcccgaa
    13261 acgcggcgag gcgctgtcat aattcccggc ctgatattca cctgcggcaa agctatattt
    13321 gagatggcct tcacgctgga aaattggcac cgatgagaat ggctgaataa agcgttgttc
    13381 actgccatcc gcttctttaa taatgaccgt gagatcaccg ctgtttgcag tggggtaaag
    13441 atcgtttatt tcaaaggcac caggctgcac ataggtgcga taaataacgt agccgttttg
    13501 ttcaatactg acttcggcgt tgcttttggc gatccctcga acgatgggtg caaagcctcg
    13561 ctggctgtcg ggcagcatct cttcatcaga ggctaacatc gcgccacgca tctgaacgct
    13621 gtcaaaaatg tcccccgcgg tcgccgtatc ccccattgtt aattgcgctt tgaaagggat
    13681 gatggcccta ctcaaagacg taccgatatt atcccactgc gctttgccac cggaatggct
    13741 ccaggtactg taattccgta accgccaagc gccaaggttc agcccgctgc gtaagttgag
    13801 gtagtagctg tcgctcttta gcgtatcttt accatcatca tgatgaacag tgccattatc
    13861 atcgacataa ctgctggaag accccgtcga gtcatattca ctgttgctcc cgctaaaact
    13921 gtaatccagc agcaacgcag ggataccttc gtcccataaa gattccggca ccgtgccacg
    13981 cgccacctgg tgcatcgccg cctgagggaa acttaatacc agcttttgtt cactgaagtc
    14041 gaaatggctt gaggccaggg gaatgatttc gtcaaacggt acgcaggctt cggcaggcac
    14101 cagattgagg gccggaaagc tttcaacgcg cacgcccatc tctgccagtt gcgtccggct
    14161 aaggcaaggc acaagggatt cccccatcgc tttgcgctgt gcttctgtgg cctctttaaa
    14221 ttccagtgtg cgggtttcca ttctctgttc attgacgtaa acagaaacac gatagatgcc
    14281 gggtaactgc tgttcctggc ctttttcgaa catcgttaaa tcgacatgct ggcccacacc
    14341 ctgaacgtcc tccaggaatt tggggttaaa ctttccggca tgtgcagaaa acgtgctggc
    14401 aacagcaacc gccaccacag agatagaaaa ggaagaaaaa gtacgtgaaa cggtcttccg
    14461 tgacattgag ctatttctcc tgaaaagata ttaatcaatc aacattttta gtgatcattt
    14521 ccgctgacga tccaaagtca ttgatggcgt tata
//
LOCUS       DAZFFG010000112        14498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFFG010000112 DAZFFG010000000
VERSION     DAZFFG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.112"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(106..858)
                     /gene="tcyN"
                     /locus_tag="ACHJ7J_003877"
     CDS             complement(106..858)
                     /gene="tcyN"
                     /locus_tag="ACHJ7J_003877"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO5002053.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(855..1523)
                     /gene="tcyL"
                     /locus_tag="ACHJ7J_003878"
     CDS             complement(855..1523)
                     /gene="tcyL"
                     /locus_tag="ACHJ7J_003878"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO5002054.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1538..2524)
                     /gene="dcyD"
                     /locus_tag="ACHJ7J_003879"
     CDS             complement(1538..2524)
                     /gene="dcyD"
                     /locus_tag="ACHJ7J_003879"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO5002055.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2629..3429)
                     /gene="tcyJ"
                     /locus_tag="ACHJ7J_003880"
     CDS             complement(2629..3429)
                     /gene="tcyJ"
                     /locus_tag="ACHJ7J_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5002056.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3517..4068)
                     /gene="fliZ"
                     /locus_tag="ACHJ7J_003881"
     CDS             complement(3517..4068)
                     /gene="fliZ"
                     /locus_tag="ACHJ7J_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO5002057.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4114..4833)
                     /gene="fliA"
                     /locus_tag="ACHJ7J_003882"
     CDS             complement(4114..4833)
                     /gene="fliA"
                     /locus_tag="ACHJ7J_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO5002058.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5153..6820)
                     /locus_tag="ACHJ7J_003883"
     CDS             complement(5153..6820)
                     /locus_tag="ACHJ7J_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO5002059.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKDDLVAASVSAAVGNEYTVSAGLSKSTAADV
                     IASLTDGATVTAAGVSNGFAAGATGDAYKFNQANNTFTYNTTSTAAELQSYLTPKAGD
                     TATFSVEIGGTKQDVVLASDGKITAKDGSKLYIDTTGNLTQNGGGTLEEATLNGLAFN
                     HSGPAAAVQSTITTADGTSIVLAGSGDFGTTKTAGAINVTGAVISADALLSASKATGF
                     TSGTYTVGTDGVVKSGGNDVYNKADGTGLTTDNTTKYYLQDDGSVTNGSGKAVYADAT
                     GKLTTDAETKAETTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSE
                     AQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7086..8492
                     /gene="fliD"
                     /locus_tag="ACHJ7J_003884"
     CDS             7086..8492
                     /gene="fliD"
                     /locus_tag="ACHJ7J_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5002060.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8517..8927
                     /gene="fliS"
                     /locus_tag="ACHJ7J_003885"
     CDS             8517..8927
                     /gene="fliS"
                     /locus_tag="ACHJ7J_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5002061.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            8927..9292
                     /gene="fliT"
                     /locus_tag="ACHJ7J_003886"
     CDS             8927..9292
                     /gene="fliT"
                     /locus_tag="ACHJ7J_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO5002062.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9369..10856
                     /gene="amyA"
                     /locus_tag="ACHJ7J_003887"
     CDS             9369..10856
                     /gene="amyA"
                     /locus_tag="ACHJ7J_003887"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5002063.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10890..11303)
                     /gene="yedD"
                     /locus_tag="ACHJ7J_003888"
     CDS             complement(10890..11303)
                     /gene="yedD"
                     /locus_tag="ACHJ7J_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO5002064.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11490..12695
                     /gene="yedE"
                     /locus_tag="ACHJ7J_003889"
     CDS             11490..12695
                     /gene="yedE"
                     /locus_tag="ACHJ7J_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO5002065.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMVVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12692..12925
                     /gene="yedF"
                     /locus_tag="ACHJ7J_003890"
     CDS             12692..12925
                     /gene="yedF"
                     /locus_tag="ACHJ7J_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO5002066.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13034..13702
                     /gene="yedK"
                     /locus_tag="ACHJ7J_003891"
     CDS             13034..13702
                     /gene="yedK"
                     /locus_tag="ACHJ7J_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO5002067.1"
                     /translation="MCGRFAQSKTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPNFIYRTDGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQDIGGKEASEIATSGCVPANQFTWHPVSRAVGNVKNQGAAL
                     IQPV"
     gene            13813..14292
                     /gene="yedL"
                     /locus_tag="ACHJ7J_003892"
     CDS             13813..14292
                     /gene="yedL"
                     /locus_tag="ACHJ7J_003892"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5002068.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILCHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFADCEPFADYRLDPHSRFLSLTLCENNELP"
     gene            complement(14430..>14498)
                     /locus_tag="ACHJ7J_003893"
     CDS             complement(14430..>14498)
                     /locus_tag="ACHJ7J_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002069.1"
                     /translation="KYSDMVKKYVIDGEELIIKYYM"
BASE COUNT         3563 a         3716 c         3577 g         3642 t
ORIGIN      
        1 tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcag gcttgaaatg
       61 gcgttccgcc ccgactgtcg aatcgtcggg gctgattttt tcttattatt gcagcagaaa
      121 cttctcgagg aactggcggg tgcgaggctg ctcgggatcg gcaaataacg cttttgcggc
      181 cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa catcccgggc
      241 aaagctcatt tcgtgtgtca caatcaccat cgtgcgcttt tcctgcgcca gctgacggat
      301 ggtgttcagg acttcgccca ccagctctgg atccagcgct gacgttggct cgtcaaacaa
      361 aatcacctca ggacgcattg ccagcgcacg cgcaatcgca acacgctgct gttgaccgcc
      421 agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg ccaacagctc
      481 gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg gcccttcaat
      541 aatgttctcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga caaacccgac
      601 gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg cagtatcaat
      661 agtgatatcg ccaacggtga tcgttcccgc ttccggttgt tccagcagat ttatgctacg
      721 tagcaatgtg gttttgccgg aaccactcgg accgataatt gccaccactt cgccaggctt
      781 tacctcaagg tcgataccgt gcagcaccgt ctgaccgtgg aattttttca ccaggttctt
      841 aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt ctcaaaatgg
      901 ttctgcaacg tcgataacac agtcgccatg atccagtaga tgagcgaagc cgccagatac
      961 atggtgaaca cctccagcgt acgcgaggta atcaactgcg cctgacggaa cagctccggc
     1021 acctggattg tcgcggccag cgacgtatct tttaccagac tgataaagct gtttgacagc
     1081 ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt ctgccacggc
     1141 gtcataccaa tactagcagc tgcttcccac tgacctttat caatagaaga aatggcggcg
     1201 cgcagtgttt cggcggcata ggcggcagtg ttgagcgaca gaccaatcat tgctgacgga
     1261 atgggatcta attcgatacc aaactgcggc aagccgtaat agatcataaa cagttgtgcg
     1321 atgagcggcg taccacgaaa aatagagata taaaaacgcg ccagccagcg cacgggccag
     1381 attggcgaca ggcgcatcag cgcgagaata aacccgagca gtaagccaaa aaacatgccg
     1441 ccgatactga gttgcagcgt atacccagcc cctttcagca ggaacggcaa ggaatcaata
     1501 accagttgta tactttcttg catgagcgtt tccggagcta aacgtgggga tgataggcga
     1561 acagcgcagg cgcaccgccg gtatgaataa acagaatcgg cccttcatct ttgaagcgtt
     1621 tctgactgat accgtcaatc agacccgcca tcgcttttcc ggtatacaca ggatcaagca
     1681 gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg ttcggcacgc
     1741 cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc tgctgatgcg gtcagctcca
     1801 gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caattgatcg gcaacggaac
     1861 gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc ccaacagcca
     1921 gtccggcgtg agttccggca ctgcccgatg cgactaccac cgatgaaata ttaaccgccc
     1981 cttcacactg ttgcgcgatt tccagcgcac tctccacata acctagcgcg ccaagagcat
     2041 tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact cgcgtcgcca
     2101 gctcttccag ttgggcattg ggatcggtca gtgcgtcgca catttcaatc tgggtattga
     2161 acagatccag caacaagcga ttgccgttgg ttaaatagtt ttctgcggtt gtgccaatag
     2221 gattttccag cagcgccacg cagtgcagac cgagtttggc agcgactgcg gcagtctgcc
     2281 gcacatggtt agactggatc gccccggcag taatcagcgt atcggcacct tcacgcagag
     2341 catctgccgc gagaaattcc agcttacgta atttattgcc gcccattgcc atgggggtga
     2401 cgtcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc ggcagatatt
     2461 cgagcggtgt tggcgcgccg ataaactcca gccgtggaaa acgggttaaa ttatgcagtg
     2521 gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct gaaaacgcgt
     2581 aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt atttggtcac
     2641 atcagcacca aaccattttt ctgaaagagc ttgcagagtg ccatcttttt gcatttccgc
     2701 aattgcatca ttcactgctt tcagcaggtc ttcatttcct ttacgcagcg ccacgccaga
     2761 ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt tctttaccag
     2821 atccagcgct gccagacggt caacgaggat cgcatcgata cggcctacgc gcagatcctg
     2881 atatttggtc gggtcatcat cataggtacg cacatcgacg ccctgaacat tctgacgcag
     2941 ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca gatcatcagc
     3001 cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccgg aaatggtgta
     3061 cggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct gattaatcac
     3121 cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg tcggttttag
     3181 tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt caaaaccggt
     3241 taatttgccg tcatctccct gaaaactgaa cggcggataa gtaccttcca gccctaccag
     3301 cagcgtgccg cgctctttaa ctttattaag cagaccttca tctgcaaaac ttttaacgct
     3361 catgcccgca atcagcgcta cggccatcac acccatcaat gcctgacgtc ccagatgtgc
     3421 taatttcata ttcaccccga atgttgttat gtctgtttgc agtgtagagc cattgtttgt
     3481 aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag aagcaggcag
     3541 gctggaggat ttctgcacaa gtccggtaca ggtttggcgc tgatagtgtt gatacttccg
     3601 taacgcgatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga ggatttcatc
     3661 aaggaaaccg tcctgcagct gatcgaggga aatattttgc tcgtgtagat gattccccaa
     3721 acggcgcaga cgaacgacat attcacgcac cgtacctggg ctcatttcag tttgctcaaa
     3781 aagaaattgc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc aatgtaaatc
     3841 accgcaaaat cggcacaatg ccgcccatga tttttgttcc gtttgccagc cattttcatc
     3901 aagcagcgtg tccaggcggg aaatctcgat tttattcact attttgccgt cgcgaactaa
     3961 ggtaatgcga tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt gtttaaagtc
     4021 tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta agaactcctg
     4081 gtagtcaaag ttaaagtgcg gcatttactg acgttataac ttacccagtt tagtgcgtaa
     4141 ccgtttaatg gcctggctgt gtaactgact gacccgcgat tccccgacct ccagcactgc
     4201 accaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca gtttttcgcg
     4261 ctccggcaac gtttcgatag cttccatcac ccgctggcgc agattactat ccagcagttg
     4321 ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat cgccgtgctc
     4381 ttcgcgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca acatttggcg
     4441 ataatcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg cgttgcggcc
     4501 aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct
     4561 gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga tacgctgcac
     4621 tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg cattaagtaa
     4681 cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg
     4741 caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg agtgtttatc
     4801 cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt tatatgagtt
     4861 atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg gtgaaatgag
     4921 gggttatttg ggggttaccg gtaaattcca tgcagaaaaa accccgccgg tggcggggaa
     4981 gcacgttgct gacaaattgc gctttatgtt gccggatgcg gcgtaaacgc cttatccggc
     5041 ctacaaaaat gtgcaaattc aataaattgc aattcacctt gtaggcctga taagtgcagc
     5101 gcatcaggca atttggcgtt gccgtcagtc tcagttaatc aggttacgac gattaaccct
     5161 gcagcagaga cagaacctgc tgcggtacct ggttggcttt tgccagcacg gagttaccgg
     5221 cctgctggat gatctgcgct ttcgacatat tggacacttc ggtcgcatag tcggcgtcct
     5281 gaatacggga ctgcgcttca gacaggttgg tagtggtgtt gttcaggttg gtgactgcgg
     5341 aatccagacg gttctgcacg gcacccaggg aagaacggaa tttgtcgatg gagctgatgg
     5401 cttcgtccag agctttcagg ggatcggcgg tggtttcggc tttggtttca gcgtcagtag
     5461 ttagttttcc tgttgcatca gcatacacag ctttaccaga accattagtt acagacccgt
     5521 catcttgtaa ataatatttt gtggtattgt cagtagttaa tcccgtcccg tcagctttgt
     5581 tataaacgtc attgccacca gatttaacaa ctccatctgt acctacggta taagtgccag
     5641 aagtaaaccc agtcgcttta ctggcggaaa gaagtgcatc agcactgatc actgctcctg
     5701 tgacattaat agccccagca gtttttgttg ttccaaagtc gccagaacct gctagaacta
     5761 ttgaagttcc atccgcagta gtaatagtag attgtacagc agcggctgga ccagagtggt
     5821 tgaaagctaa accattgagg gtagcttctt ctaaagtacc accaccgttt tgggttaaat
     5881 tccctgtggt gtcaatataa agtttagacc cgtcttttgc tgtgattttg ccatcactag
     5941 ccagaacaac atcctgcttg gtgccaccaa tttcaacgga gaaagttgcg gtatcccccg
     6001 ccttaggcgt gaggtaagat tggagttctg ccgctgttga ggtggtattg taagtaaaag
     6061 tgttgtttgc ttgattgaat ttataagcat ctccagttgc ccctgcagca aaaccattgc
     6121 ttacaccagc cgcagttact gtcgcaccat ctgtgagact agcaataaca tcagcagcag
     6181 ttgatttcga caggccagca gagacagtgt attcattacc taccgcagct gaaactgatg
     6241 cagcgaccaa atcatcttta gtcgctgcag tattagccac agccccgcca ccattcacat
     6301 taaacccact cagccccagc gtatcagagt caattttctt cagatcaata gtgatagtct
     6361 ggccgtcatt cgcaccaacc tgaattttca ttgaaccgtc tttcgccagt acgttcacgc
     6421 cgttgaactg ggtctggcca gatacgcggt caatttcgtc cagacgggat ttgatttcgt
     6481 cctgaatgga gtccagatcc gaatcggagt tagtcccggt agaagcctga accgtcagtt
     6541 cacggatacg ctgtaagttg ttgttgattt cggacagcgc gccttcggtg gtctgcgcaa
     6601 cagaaatacc gtcgttggcg ttacgtgcag cctgagtcag gcctttaatg ttagaagtaa
     6661 aacggttagc aatcgcctga cctgcggcgt catccttcgc gctgttaata cgcaagccag
     6721 aagacagacg ctcgatagaa ctcgacagcg cagactggtt cttgttgata ttattttgag
     6781 tgatcagcga gaggctgttg gtattaatga cttgtgccat gattcgttat cctatattgc
     6841 aagtcgttga ttacgtattg ggtttccacc cgtcggctca atcgccgtca accctgttat
     6901 cgtctgtcgt aaaacaacct ttagaatttt tttcacaaac agccattttt tgttagtcgc
     6961 cgaaatactc ttttctctgc cccttattcc cgctattaaa aaaaacaatt aaacgtaaac
     7021 tttgcgcaat tcaggccgat aaccccggta ttcgttttac gtgtcgaaag ataaaaggaa
     7081 atcgcatggc aagtatttca tcgctgggag tcgggtcagg cctggattta agttcaatcc
     7141 ttgatagcct caccgccgcg caaaaagcga cgctaacccc tatttcaaat cagcaatcgt
     7201 cgtttaccgc taaacttagc gcctacggta cgctgaaaag cgcgttgacg actttccaga
     7261 ccgccaatac tgcattgtct aaagccgatc ttttttccgc caccagcact accagcagca
     7321 ccaccgcatt cagtgccacc actgcgggta acgccatcgc cgggaaatac accatcagtg
     7381 tcacccatct ggcgcaggcg caaaccctga ccacgcgcac caccagagac gatacgaaaa
     7441 cggcgatcgc caccagcgac agtaaactca ccattcaaca aggcggcgac aaagatccga
     7501 ttaccattga tatcagcgcg gctaactcat cgttaagcgg gatccgtgat gccatcaaca
     7561 acgcaaaagc aggcgtaagc gcaagcatca ttaacgtggg taacggtgaa tatcgtctgt
     7621 cagtcacatc aaatgacacc ggccttgata atgcgatgac actctcggtc agcggtgatg
     7681 atgcgctaca aagttttatg ggctatgacg ccagtgccag cagcaacggt atggaggtct
     7741 cggtcgccgc ccagaatgcg cagctgacgg tcaacaacgt cgccattgag aacagcagca
     7801 acaccatcag cgacgcgctg gaaaacatca ccctgaacct gaacgatgtc accacgggca
     7861 accagacgct aaccatcact caggacacct ccaaagcgca aacggcgatt aaagattggg
     7921 tgaatgccta taactcgcta atagatacct tcagcagcct gaccaaatac accgccgtag
     7981 atgcgggagc tgatagccag agttctagca atggcgcact gctcggcgac tccacgctgc
     8041 ggacgattca gacgcagttg aagtcgatgc tgagtaatac cgtcagttct tccagctata
     8101 aaacgttggc gcagattggt atcacgaccg atcccagcga tggcaaactg gaactggatg
     8161 ccgacaaact caccgctgca ctgaaaaaag atgccagcgg cgtaggtgca ttgattgttg
     8221 gcgatggtaa aaaaaccggc atcacaacca ccatcggcag caacctgacc agttggcttt
     8281 cgacaacggg cattattaaa gccgctaccg atggcgttag taagaccctg aataaattaa
     8341 ctaaagacta caacgccgcc agcgatcgca ttgatgcgca ggtcgctcgc tacaaagaac
     8401 aatttaccca actggacgtt ttaatgacct cgttaaacag caccagcagc tacttaacgc
     8461 agcagttcga aaacaacagt aattccaagt aatcaatatt catcgggaga caggtcatgt
     8521 acgcggcaaa aggcacccag gcctatgcac aaattggcgt cgaaagcgcc gtaatgagcg
     8581 ccagccagca gcagctggtc accatgctat ttgatggagt gctgagcgca ctggttagag
     8641 cgcgcctgtt tatgcaggac aacaatcagc aaggcaaagg cgtctctttg tcaaaagcga
     8701 tcaacatcat tgagaacgga ctgcgggtga gtcttgatga agagagcaaa gacgaactaa
     8761 cccaaaactt gattgctctt tatagctata tggtcaggcg tttgctgcaa gccaatttac
     8821 gcaacgatgt ctccgcagtc gaagaagtgg aaacattaat gcgcaatatt gccgatgcct
     8881 ggaaagagtc gttactctcc ccttctttga ttcaggaccc agtctgatga accatgcacc
     8941 gcatttgtat ttcgcctggc aacaactcgt cgaaaaaagc cagctcatgt tacgcctggc
     9001 aacggaagaa caatgggacg aactcatcgc cagcgaaatg gcgtatgtta atgcggtgca
     9061 ggagattgca catttgactg aagaggttga cccgtccacc acgatgcagg agcagctccg
     9121 cccgatgctg cgcctgattc tcgacaacga aagcaaggta aagcagttat tacagattcg
     9181 gatggatgaa ctggcgaaac tggtcggtca gtcatcggtg caaaaatcgg tgttaagtgc
     9241 ctatggcgat cagggcggct ttgtgctggc tccgcaggat aacctctttt gaatctgaac
     9301 gagtcgatgc tcgcgaataa tccgattacg gctacgcttc taatgttccc cttgaatgga
     9361 gtcgaagaat gcgtaatccc acgctgttac aatgttttca ctggtattac ccggaaggcg
     9421 gtaagctctg gcctgaattg gccgagcgcg ccgacggttt taatgatatt ggtatcaata
     9481 tggtctggtt gccgcccgcc tataaaggcg catcgggcgg gtattcggtc ggctacgact
     9541 cctatgattt atttgattta ggtgagtttg atcagaaagg cagcatccct actaaatatg
     9601 gcgataaagt acaactactg gccgccattg acgccctgaa acgtaatgac attgcggtgc
     9661 tgttggacgt ggtagtcaac cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc
     9721 agcgtgtaaa tgctgacgac cgtacgcaaa ttgatgaaga aatcattgag tgcgaaggct
     9781 ggacgcgtta caccttcccc gcccgtgccg ggcaatactc gcagtttatc tgggatttca
     9841 aatgttttag cggtatcgac catatcgaaa accctgacga agatggcatt tttaaaattg
     9901 ttaacgacta caccggcgaa ggctggaacg atcaggttga tgatgaatta ggtaatttcg
     9961 attatctgat gggtgagaat atcgattttc gcaatcatgc cgtgacggaa gagattaaat
    10021 actgggcgcg ctgggtgatg gaacaaacac aatgcgacgg ttttcgtctt gatgcggtca
    10081 aacatattcc ggcgtggttt tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa
    10141 agccgctgtt tattgtggca gagtactggt cgcatgaagt cgataaactg caaacgtata
    10201 ttgatcaggt ggaaggcaaa accatgctgt tcgatgcgcc gctgcagatg aaattccatg
    10261 aagcatcgcg catggggcgc gactacgaca tgacgcagat tttcacgggt acattagtgg
    10321 aagccgatcc tttccacgcc gtaaccctcg ttgccaacca cgacacccaa ccgttgcaag
    10381 ccctcgaagc accggtcgaa ccgtggttta aaccgctggc gtatgctcta attttgttgc
    10441 gggaaaatgg cgttccttcg gtattctatc cggacctcta cggtgcgcat tacgaagatg
    10501 tcggtggtga cgggcaaacc tatccgatag atatgccaat aatcgaacag cttgatgagt
    10561 taattctcgc ccgtcagcgt ttcgcccacg gtgtacagac attatttttc gaccatccga
    10621 actgcattgc ctttagccgc agtggcactg acgaatatcc cggctgcgtg gtggtcatgt
    10681 cgaacgggga tgatggcgaa aaaaccattc atctgggaga gaattacggc aataaaacct
    10741 ggcgtgattt tctggggaac cggcaagaga gcgtaataac cgacgaaaac ggcgaagcaa
    10801 ccttcttttg caatggcggc agtgtcagcg tgtgggttat cgaagaggtg atttaaattc
    10861 atccccggcg gcaagccggg gagatttcat tacggcagtg gcgtcggtaa cggcgcttta
    10921 tccagcgcgg cggcacactc tgctgtcggg cgatccacac gctccatcgt cataccatcg
    10981 tactcaatgg tgttaccgtc acgctcaacc tcgtacagct cacgttttac ggtaacgtta
    11041 gtgagatcat cagacatcaa tgtcaactta cccggcacgg caatcacccg ctgccactga
    11101 cggcaatcca gggtatcgcc ctctttagtg acaatgaggc tgcctatcgc ttccgggctg
    11161 accagcgcac gttgcggccc tttggtctgc cagtagcccg ccagccagtc cggcgcgggg
    11221 gttttcacga cattgttata gttttcgact tcggcacagc ccgccagcaa catgagcgcg
    11281 cctgcaattg cgagtttttt catcattcgt cctgcatgag aagaaaagag gattgtggca
    11341 ttaaagccct gatgtcgcca gcctttccag gttcagagag aaatttgatc taactattat
    11401 ttttgtgaat tattaccccg aaaataaatt tgcatggtgt atgattcgcg actcttgttt
    11461 caaaccttct gagttcagag gctacattca tgtcatggca gcaattcaaa cacgcctggt
    11521 tgattaaatt ctgggcgccc atccccgcgg tcatcgcggc gggtattctc tccacttact
    11581 attttggcat tactggcacc ttttgggctg tcacgggtga atttacccgt tggggcggtc
    11641 agctcctgca gctgtttggc gtccatgctg aagagtgggg ttattttaaa attatccatc
    11701 tggaaggatc gccattaacc cgcatcgacg ggatgatgat cctcggtatg tttggcggct
    11761 gctttgccgc agcgctgtgg gccaacaatg tcaaactacg catgccgcgt agccgtatcc
    11821 gcattatgca ggccatcatt ggcggcatta tcgccggttt tggcgcgcgt ctggcaatgg
    11881 gctgtaacct ggcggcattc tttaccggta ttcctcagtt ctcgctgcat gcctggttct
    11941 ttgccatcgc cactgccatt ggttcatggt ttggcgcgcg ctttaccctg ctgcccatct
    12001 tccgtattcc cgtgaaaatg cagaaagttt ctgccgcctc accgctgacg caaaaaccgg
    12061 atcaggcgcg gcgtcgtttt cgtctcggga tggtggtctt tttcggcatg ctgggctggg
    12121 cgctgctcac agcgatgaac caacccaaac tggggctggc aatgctgttt ggcgtcggct
    12181 ttggtttact gattgaacgt gcgcaaatct gctttacttc ggcgttccgc gatatgtgga
    12241 tcaccggacg tacccatatg gcgaaagcaa tcattattgg tatggcggtg agtgccatcg
    12301 ggatcttcag ttacgtacag ttaggcgttg aacccaaaat catgtgggcg ggaccaaacg
    12361 cggtaattgg tggtttactg tttggttttg gcatcgtgct ggctggcggc tgcgaaaccg
    12421 gctggatgta ccgcgcggta gaaggccagg tgcactactg gtgggtcggt ctgggcaatg
    12481 tgatcggctc aacgattctg gcgtattact gggatgattt cgctccggcg ctggccaccg
    12541 actgggacaa aatcaacctg ctgaaaacct ttggtccgat gggtggcctg ctggtgacat
    12601 atttgctgtt gtttgctgcg ctgatgttga ttatcggctg ggaaaaacgc ttcttccgcc
    12661 gtgcggcacc gcagactgct aaggagatcg catgaaaaat atcgttcctg attaccgcct
    12721 tgatatggtg ggtgaaccct gcccttatcc ggcagtcgca acccttgagg cgatgccgca
    12781 gttaaaaaaa ggggaaatcc tggaagtggt gagcgactgt ccgcagtcga tcaataatat
    12841 tccactggat gcgcgtaatc acggctatac ggtgctggat attcagcaag acgggccgac
    12901 cattcgttat ttaattcaga agtaatccct cattcccgct gtgacccaca gcgggaacat
    12961 cgttaccttt gtatcacata gattttaccc accccctctc ctattctttt cagtatgatc
    13021 tcaggaggtt tctatgtgtg gacgctttgc ccaatccaaa acgcgtgaag attaccttgc
    13081 gcttctcgcg gaagatattg aacgcgatat tccctatgat cccgaaccca ttggcagata
    13141 caacgtcgcg ccgggaacca aagtcctact gctcagtgaa cgtgatgaac accttcatct
    13201 ggatccggtt ttctggggat atgctcccgg atggtgggat aaaccgccgc tgattaacgc
    13261 ccgcgtagaa actgcggcaa ccagtcgtat gtttaaaccg ctctggcaac atggtcgggc
    13321 aatctgtttt gccgatggct ggtttgagtg gaaaaaagaa ggcgacaaaa agcagcctaa
    13381 ttttatctat cgtactgatg gacaacctgt ttttatggcc gcgataggta gcacaccgtt
    13441 cgagcgtggt gacgaagccg aaggattttt gatcgtcact gcagcagcag accagggtct
    13501 ggtagatatt catgaccgcc gcccgctggt tctgtcgcca gaagctgcgc gggaatggat
    13561 gcggcaagat attggcggta aagaagcctc agaaatcgct accagtggtt gtgtgccagc
    13621 aaaccagttc acctggcacc cggtatcgcg cgcggtgggt aatgttaaaa accagggtgc
    13681 agcgttaatt caacctgttt gataagcctg gagattattg atcaacaata ctgcgtcata
    13741 agtaatctct attagacaaa gatttcatta cctgttggca tattgcaaaa ataacaccaa
    13801 tacggaatcg tcatgttcac gattaaaaca gatgatctca cccatccagc agtgcaagca
    13861 ttagtggctt accatatttc cggcatgctg cagcagtctc cccctgaaag cagtcatgct
    13921 ttagatgtgc aaaaattacg taacccgaca gtgacattct ggtcagtatg ggaaggcgaa
    13981 caactcgcag gaattggtgc gctgaagttg ctggatgata aacatggcga actgaaatca
    14041 atgcgtaccg cgccaaatta tttacgtcga ggtgtcgcca gtctgatttt atgccacatt
    14101 ttgcaggtcg cccatgacag atgccttcat cgcctgagct tagaaacggg tacacaggct
    14161 ggatttacgg cctgccatca actttatttg aagcatggtt tcgctgattg cgaaccgttt
    14221 gctgattatc gacttgatcc acacagtcga tttttatcat tgacgctatg cgaaaataat
    14281 gaattgccat gagccagacg cagcacattc ttgcattcga cgtgctgcgc ctttatttat
    14341 caccagcagg aaacgccttg tccatagacg ccccttccac atgcgccaca agaaacctct
    14401 attccagtga cacaattgcg cctaattaat tacatataat atttaattat gagttcctca
    14461 ccatctatta catacttttt aaccatatcg gaatattt
//
LOCUS       DAZFFG010000113        14423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFFG010000113 DAZFFG010000000
VERSION     DAZFFG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.113"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            199..2340
                     /locus_tag="ACHJ7J_003894"
     CDS             199..2340
                     /locus_tag="ACHJ7J_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO5002070.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTLKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2404..4296)
                     /gene="parE"
                     /locus_tag="ACHJ7J_003895"
     CDS             complement(2404..4296)
                     /gene="parE"
                     /locus_tag="ACHJ7J_003895"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO5002071.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4325..4906)
                     /gene="yqiA"
                     /locus_tag="ACHJ7J_003896"
     CDS             complement(4325..4906)
                     /gene="yqiA"
                     /locus_tag="ACHJ7J_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO5002072.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4906..5733)
                     /gene="cpdA"
                     /locus_tag="ACHJ7J_003897"
     CDS             complement(4906..5733)
                     /gene="cpdA"
                     /locus_tag="ACHJ7J_003897"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO5002073.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5758..6180)
                     /locus_tag="ACHJ7J_003898"
     CDS             complement(5758..6180)
                     /locus_tag="ACHJ7J_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO5002074.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6181..6810)
                     /gene="nudF"
                     /locus_tag="ACHJ7J_003899"
     CDS             complement(6181..6810)
                     /gene="nudF"
                     /locus_tag="ACHJ7J_003899"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO5002075.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7015..8496
                     /gene="tolC"
                     /locus_tag="ACHJ7J_003900"
     CDS             7015..8496
                     /gene="tolC"
                     /locus_tag="ACHJ7J_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO5002076.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8644..9315
                     /locus_tag="ACHJ7J_003901"
     CDS             8644..9315
                     /locus_tag="ACHJ7J_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO5002077.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9321..10481
                     /locus_tag="ACHJ7J_003902"
     CDS             9321..10481
                     /locus_tag="ACHJ7J_003902"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5002078.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10519..11307)
                     /gene="ygiD"
                     /locus_tag="ACHJ7J_003903"
     CDS             complement(10519..11307)
                     /gene="ygiD"
                     /locus_tag="ACHJ7J_003903"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO5002079.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11450..12223
                     /gene="zupT"
                     /locus_tag="ACHJ7J_003904"
     CDS             11450..12223
                     /gene="zupT"
                     /locus_tag="ACHJ7J_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO5002080.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12281..12451)
                     /gene="yqiD"
                     /locus_tag="ACHJ7J_003905"
     CDS             complement(12281..12451)
                     /gene="yqiD"
                     /locus_tag="ACHJ7J_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO5002081.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(12713..13366)
                     /gene="ribB"
                     /locus_tag="ACHJ7J_003906"
     CDS             complement(12713..13366)
                     /gene="ribB"
                     /locus_tag="ACHJ7J_003906"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO5002082.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCVTEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(13470..13618)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            13740..14039
                     /gene="ubiK"
                     /locus_tag="ACHJ7J_003907"
     CDS             13740..14039
                     /gene="ubiK"
                     /locus_tag="ACHJ7J_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO5002083.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(14074..14274)
                     /gene="glgS"
                     /locus_tag="ACHJ7J_003908"
     CDS             complement(14074..14274)
                     /gene="glgS"
                     /locus_tag="ACHJ7J_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO5002084.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT         3682 a         3812 c         3489 g         3440 t
ORIGIN      
        1 aattgaattg cattaacctc tcaaaaaaac acatgtagca attttttgtt atttctcatt
       61 tactactaca ttaccgcatg gttgaatgat tctcaattac atatccgatg gtgaaataac
      121 aaacggatag tgcttagtga gcaacaagaa acaattttca ctcacgacaa tccgtgagtt
      181 tttgggataa cagactaaat ggctaagttc acaccttcat tctcaggaat caaaggtcgg
      241 gcgctctttt cactgctctt tgcagcacca atgattcatg caaccgacac tgcaacgacc
      301 aaagatggcg aaacaatcac tgttacagcg gatgcaaata ccgcaactga ggcgaccgat
      361 ggttatcaac ctctgagcac ttccacggcg acattaaccg atatgccgat gctggatatc
      421 ccgcaggtgg tcaatacggt tagcgatcag gttctggaaa atcagaatgc aacgacgctg
      481 gatgaagcgc tttataacgt cagtaacgtg gtacagacca atacattagg cggaactcag
      541 gacgcctttg tacgtcgtgg gtttggtgct aaccgggatg gctccatcat gaccaacggc
      601 ctgcgaactg tacttcctcg cagtttcaac gccgccacag aacgtgtgga agtgctaaaa
      661 ggtccggcct ccacgctgta tggcattctc gatcctggtg gattgattaa cgtcgtgacc
      721 aagcgcccgg aaaaaacatt ccatggttct gtctcagcca cgtcctccag ttttggcgga
      781 ggcactgggc aacttgatat cacaggtccc attgaaggca ctcagctggc ataccgcctg
      841 acgggggaag tgcaggatga agattactgg cgaaacttcg gtaaagagcg cagtacattt
      901 attgccccgt cactcacctg gtttggtgat aatgcaacag taaccatgct ctattcccat
      961 cgggactata aaactccgtt cgatcgtgga acgattttcg accttacgac gaaacagccc
     1021 gtaaacgttg atcgaaaaat acgttttgac gaaccgttta atattacaga tggtcagtcc
     1081 gatctggcgc aactcaacgc agaatatcat ctcaatagcc agtggacagc gcgctttgat
     1141 tacagctaca gccaggataa atacagcgac aatcaggctc gcgttaccgc gtatgatgca
     1201 acgacaggaa cgctgacacg gcgtgttgat gcaactcagg gatctaccca gcgtatgcat
     1261 tctactcgtg cggatctgca agggaatgtt gatattgctg ggttctataa tgagattctg
     1321 ggtggggtgt catatgaata ttatgatctt ctgcgcacag atatgattcg ctgtaaaaac
     1381 gctaaagatt tcaatatata caaccccgtt tatggcaata ccagcaaatg cacaacggtt
     1441 tcggcgtcgg acagtgatca gacgctcaaa caggagagct actcagctta tgcacaggat
     1501 gcgctctatc tgaccgataa ctggattgcc gtcgccggga tccgctatca gtattacacg
     1561 cagtatgcgg gtaaaggccg tccttttaat gtcaatactg acagccgcga tgaacaatgg
     1621 acgcccaaac tggggttagt ctacaaactg acgccatcgg tatccttatt tgctaattat
     1681 tcgcaaacat ttatgccgca atcgtcaatt gccagctaca tcggagatct tccaccggaa
     1741 tcatctaatg cttacgaagt cggggcaaaa ttcgagctgt tcgatggtat aaccgcagat
     1801 attgcgctgt ttgatatcca taaacgtaac gtgttgtata ccgaaagtat tggtgatgaa
     1861 accatcgcca aaacggcagg ccgcgttcgt tcaagagggg tagaagtcga ccttgcggga
     1921 gcattaactg aaaacattaa tatcattgcc agctacggct ataccgatgc taaggttctg
     1981 gaagatcctg attatgcagg gaaaccattg ccgaatgttc ctcgtcatac cggttcgcta
     2041 ttcctgacct atgacattca taacatgcct ggcaataaca cactgacgtt tggcggtggt
     2101 ggacatggcg taagccgtcg ttcggcaacc aatggggctg actattatct gccaggctat
     2161 ttcgttgccg atgccttcgc cgcatacaaa atgaaattgc agtatccggt cacactgcaa
     2221 ttaaacgtca aaaacctgtt tgataaaacg tattacacct cttccatcgc cacaaataat
     2281 ctggggaatc agattggcga tccgcgtgaa gtgcaattca cggtgaaaat ggaattttga
     2341 tcaaaaaagc ccggtataca cttgtatacc gggctatgtt ttattcttct ggcgggagga
     2401 ttatcagacc tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc
     2461 cgagcgtttc ttcgccagca gcatatccat catcgcgtca gtacgctgat cgtcttcatc
     2521 atcgatagtc agctgcacca gacggcgagt gttcggatca agcgtggttt cgcgcaattg
     2581 catcgggttc atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg
     2641 ttttaattgc tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt
     2701 cccgagatca atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa
     2761 cgcgcggaag tgttttacaa acaaagcgca gagcagcgtg gcaatgtgca gaccatcgga
     2821 gtcagcatcc gccaggatac agatcttgcc gtaacgaagc tggctcaaat cgtcgctgtc
     2881 tggatcgata ccaatcgcta ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc
     2941 ggaagaaact tcccaggtgt taaggatctt acctttcagc ggcatgatcg cctgatattc
     3001 acgatcgcgc gcctgcttgg cagatccgcc tgcggagtca ccttccacaa ggaacagttc
     3061 ggtacggtta agatcctgcg cggtacagtc agccagtttg ccaggcagcg ccgggccgct
     3121 ggtcagcttt ttacgcacca cttttttagc cgcacgcata cgacgctggg cgctggaaat
     3181 cgccatctct gccagcaact ccgccgcctg aacgttctgg ttcagccaca ggataaaggc
     3241 atctttcacc acgccagaaa cgaatgccgc gcactgacgc gaagagagac gctctttggt
     3301 ctgcccggca aactgcggat cctgcatttt tactgacagc acataggcgc agcgatccca
     3361 gatatcttcc gccgacaact ttacaccgcg cggcaaaatg ttgcggtatt cacagaactc
     3421 acgcatcgcg tccagcaggc cctgacgcag gccattaaca tgggtaccgc cctgcatcgt
     3481 tgggatcagg ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag
     3541 cgcccagtcc accgcttcag tatcgccagc gaaattaccg ataaacggtt tttctggcag
     3601 cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca
     3661 gcgctgttcg gtgttgttga tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac
     3721 tgctttggct ttcagcacat gcgtcaggcg tgaaacagaa aaacgcgggc tatcgaagaa
     3781 ggtttcatcc ggccagaaat ggacgctggt gccggtattg cgtttaccgc aagtgccgac
     3841 aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc
     3901 gcggcgcacg ttaacttcta cgcgcttcga cagggcgtta accaccgaaa tccccacgcc
     3961 atgcaggccg ccagagaact ggtaattttt gttagagaat ttaccgcctg catgcagacg
     4021 gcaaagaatc agttcaaccg ccggtacacc ctcttccggg tgaatatcca ccggcatccc
     4081 gcgcccatcg tcaataactt ctaacgactg gtcagcatgt aaaataacgt ccacgcgttt
     4141 tgcgtgaccc gccagtgctt catccacact gttatcaatg acttcttgcc ccaaatggtt
     4201 agggcgagtg gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag
     4261 tacctcaatg gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg
     4321 atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc
     4381 ccgtgaatgc gtggttgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca
     4441 ccgcctggcg gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg
     4501 gcgcttccag cgggtcaatc tgcatgactt taagatcgta aatatggcgt gactctagca
     4561 catattgctg cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcagttcaa
     4621 acgggcgcac cgccgggttt accaccactg cgggcagcat aaaacattgt gacaaccagg
     4681 tggcgtaata tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca
     4741 ggacaatgga ttccagcagc tctgccgcgt cggaaggata cggcggcaac tgcggaatga
     4801 tcatctcaac gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag
     4861 agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga
     4921 agcggtatca ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc
     4981 atcagcatgt aactcgagag tacgccagcc gggcgcgata gtatccagcg taaagttgga
     5041 acagtgcggc ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca
     5101 gtcgagatcc agctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt
     5161 cgccagcacg gtatccagtt cgcccgcgtt acgcaggctg tgttgatcaa gccaactaca
     5221 acccgcaggt agcggatgat gatgcagcag cagcaacgta tggcgctctg gcgcatcagc
     5281 cagtttacgt tccagccact caagctgaaa ctcgcttagc tcaccgtgcg gcacgccaaa
     5341 cacctggcta tccagcaaca ggatttgcca ttgctcacca ataaacaccc gcttcgccgg
     5401 ggagataccc gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg
     5461 cagccagacg cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc
     5521 agaggattga tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg
     5581 tggacgaatc gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaagagggc
     5641 ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt aaaatcctga ctctggcctc
     5701 accagccaga ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta
     5761 gtaaaccgga atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa
     5821 ctggttaatt tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg
     5881 cgctttgaag cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata
     5941 cagacgcacc gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat
     6001 tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc
     6061 cacctgatag cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg
     6121 tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat
     6181 ttatgcccac tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac
     6241 cgacgctgcg ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac
     6301 cacatgaacg cgaatatctt cgttttcatc agccagaccg tgaataccgc ttgcggtcgt
     6361 ggcgtccact tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag
     6421 gaaacttaac accggtttgg tccgtttgac tatcagcccc gcctcttcaa tcgcttcgcg
     6481 acgggcaaca tcttccacac tttcaccctc ttctatcatc ccggcaacca tctccagtag
     6541 ccagggggtt tcgctagtgt cgtacgcggc aattcgaatc tgctcaatca gcacaacttc
     6601 atcacgcact gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg
     6661 ccgtacctca tgactcattt gcccgttgaa tagacgatga cgaaatctat aaagatctaa
     6721 tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa
     6781 tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc ctggcactaa tagtgaatta
     6841 aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt catgtgttaa attgaggcac
     6901 attaacgccc tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg
     6961 caataatttt acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag
     7021 aaattgctcc ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc
     7081 gagaacctga tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct
     7141 gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca
     7201 cagctaggtt taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc
     7261 aactctaacg cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa
     7321 tggcgtgcgt taacgctgca ggaaaaagca gcagggattc aggacgtcac atatcagacc
     7381 gatcagcaaa ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt
     7441 gacgttcttt cctatacaca ggcgcaaaaa gaagcgatct accgtcaatt agatcaaacc
     7501 acccaacgtt ttaacgtggg cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag
     7561 tacgataccg tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag
     7621 cagctgcgcc agatcaccgg taactactat ccggaactgg cggcgctgaa tgtcgaaaac
     7681 tttaaaaccg acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac
     7741 ctgtcgctgt tacaggcacg cttgagccag gacctggcgc gcgagcaaat tcgccaggcg
     7801 caggatggtc acttaccgac tctggattta acggcttcta ccgggatttc tgacacctct
     7861 tatagcggtt cgaaaacccg tggtgccgct ggtacccagt atgacgatag caatatgggc
     7921 cagaacaaag ttggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg
     7981 caggtgaaac aggcccagta caactttgtt ggtgccagcg agcaactgga aagcgcgcat
     8041 cgtagcgtcg tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcttc tatcagtagt
     8101 attaacgcct acaaacaagc cgtggtttcc gcccaaagct cattagacgc gatggaagcg
     8161 ggctactcgg tcggtacgcg taccattgtt gatgtgttgg atgcgaccac cacgctgtac
     8221 aacgccaagc aagagctggc gaatgcgcgt tataactacc tgattaatca gctgaatatt
     8281 aagtcagccc tgggtacatt gaacgagcag gatctgctgg cactgaacaa tgcgctgagc
     8341 aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt
     8401 gctgatggtt atgcgcctga tagccctgca cccgtcgttc agcaaacatc cgcacgcact
     8461 accaccagta acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc
     8521 gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc
     8581 ttcaatttcg accagccatc ctctattctg atgggtattt accactggtc ccggaagaca
     8641 aaaatgaaac ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcacgc
     8701 catctgacac cagtcgctct cgcagttgcc actgttttta tgctggctgg ctgtgaaaag
     8761 agtgatgaaa cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc
     8821 aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg
     8881 ccgaaatacg ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgccagcag
     8941 gcaccagccc aggctggcat ggcaccagaa aaccaggcgc aggcccagca atccagcggg
     9001 agtttctgga tgccgctgat ggccggttac atgatgggtc gtctgatggg cggcggtgcg
     9061 ggatttgcac agcagccgct gttctcctcg aaaaacccag ccagcccggc ttacggtaaa
     9121 tataccgacg cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta
     9181 ccgaagacag ccatggcacc aaaaccggcg accaccacta ccgttacccg tggcggtttt
     9241 ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg caaccggtac ctcttctcgt
     9301 tcaatgggtg gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg
     9361 agaaagccca cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg
     9421 aagatgctta ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg
     9481 aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga
     9541 ccaaattccg cattccaaaa cacacctgga gttttgtgcg ccagtcatgg ctgacgcacc
     9601 agccatcgct ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa cctaaacttc
     9661 tggaaaataa cgccgatacg ccaacgtcac tatacgaggc ggcgttcttt cagtggatct
     9721 ggctggaaga tcagcttaac gccggtaact tgccggaggg cagcgaccag tttaacagtc
     9781 tgcaagaaaa actgatcgat cgcttcgttg agctgcgtga acagtatggc ttccagttgc
     9841 tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg taccattcag tatttgcagg
     9901 actgcgcgac ggaagctgaa atcgctaccg agttcctcta cattgacgat atcgggttag
     9961 gtgaaaaagg tcagttcacg gatttacagg atcaggtaat ttccaacctg ttcaagctgt
    10021 atccgtggga atttatgttg cgtgagatgt tttccaccaa gctggaggat gcaggcgtac
    10081 gctggctgga accggcgtgg aagagcatta tctccaacaa ggcacttcta ccgctactgt
    10141 gggagatgtt cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc
    10201 cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga
    10261 tcattgagaa cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga
    10321 ttgttcagca attccacccg ttaccgaaat tcggcgacag ctatatgctg attggtagct
    10381 ggctggtgaa cgatcaaccc gccggaattg gcattcgtga agaccgtgca ttgatcaccc
    10441 aggatatgtc tcggttttat ccacatattt ttgttgaata atcaatgatg ccggatggca
    10501 ttcgccatcc ggtaattgtt agcctatctg caccgacagc atacttaaac tgcccatttc
    10561 tataccatcg accggaatgg taattggctc ctgcccatcc cacgcaccta acacatacaa
    10621 caacggcaga tagtgctctg gcgttgggtt cgataacgcg ccaccttcat ggtcgagata
    10681 attcaccaga ggatgctgct ctactggccc ttgccacgtc agattcgctt tcacatactc
    10741 attaaacgac gtcgcccacg gatacggtga actatcaccg tgccacttca ctgtacgcag
    10801 gttatgcacc acgttgccgc tggcgaccaa cattattcct tcatcacgca gcgccgcgag
    10861 ttttcgcccc atttcgaaat gccaggcggc aggtttgcta ctatcgatac tcaactgcac
    10921 catcgggata tcggcgtcag gatacatctt aatcagcacg ccccaggaac cgtggtcaaa
    10981 gccccaggct tctttatcca gcgtcaccgg gaccggcgct aacagctcaa ccagatgctg
    11041 tgccagcgca ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc
    11101 accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa
    11161 ccagtgagcc gaaactacca caatcgcttg cgggcgtggc aatgtcatcc ccaacgtctg
    11221 ccagctgcgg gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa
    11281 caatgctggc atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat
    11341 atcctcacaa tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc
    11401 agttattttg acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc
    11461 tctcattctg accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct
    11521 cgggcaaaaa ccctcgaacc gcttactggc gttttcgcta ggatttgcgg cggggatcat
    11581 gttgctcatc tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc
    11641 tgtgttgggt tatggaatgt ttatcttcgg tctgcttggc tattttggcc tggaccgcat
    11701 gttgccacat gcgcatccgc aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc
    11761 gatcaagcgc acagccattc tgctcactct cggcatcagt ctgcataact tcccggaagg
    11821 gattgccacc tttgtcacgg caagcagcaa cctggagctg ggatttggca tcgcactggc
    11881 cgtcgcgttg cacaatattc ctgaaggtct ggcggttgca ggcccggttt atgcagcaac
    11941 gggttctaaa cgcacggcaa ttctgtgggc ggggatttct ggattggcag aaattcttgg
    12001 tggtgtgctg gcgtggttaa tcctcggtag catgatttcc cccgtggtca tggcggcaat
    12061 catggcggcg gttgcaggaa ttatggtggc gctctcggtt gacgaattaa tgccgctcgc
    12121 caaagagatt gaccctaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat
    12181 gggattcagt ttagtgctgc tacaaacggc gggaattggt taaaagcaac atcagaagtt
    12241 gtccagcgca tggacaactt ctgctagttt cactaaagcc ttattctacc ttttcggctg
    12301 tctcttctct cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct
    12361 ggcgaaatgc ccgacaataa cgtttcaaat gcaggaaata acccaccaca accagaacaa
    12421 tcaatacaat ccagtaccag gcgataaaca tccttttacc cttaatattt aacaatacta
    12481 aacatttagc gcataaattt cacatatcct ttttcggata tattcatatg gtcgcataat
    12541 aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt
    12601 ttctcataga aagagaagcg gaattaacat cttgcatagc agggaaaaat aaaaaccgaa
    12661 gcctgctaca acttcggttt cttgattaag gcagtaattt aagcagcggt tttcagctgg
    12721 ctttacgctc atgtgcctga cggtatgcca ccaggtcttc aatagtcacg agcgccatat
    12781 tgtgtttatt ggcaaactca atacactctg gtgcacgcgc catcgtgcca tcgtcattag
    12841 tcagctcaca cagcacacca gccggtttaa agcctgccag cgtcatcaga tcaatagtcg
    12901 cttcagtatg accgccacgc gtcagtactc cacctgcctg cgcgcgaagt gggaaaacgt
    12961 ggcccgggcg attcagatca gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg
    13021 taatacggtc agcggcagaa acaccggtag tcacaccttc agccgcttca atggtcacgg
    13081 taaaaccggt gccataggcg ctggtgttat tttctaccat cattggcaga tcgagttgtt
    13141 tacggcgatc ttcagtaacg cacaggcaaa caataccgct accgtggcga atggtcagcg
    13201 ccatctgctc aacagtcatg gtttctgccg ggaagatcat atcaccttcg ttttcacggt
    13261 cttcatcatc aagcaccatt acaccgcgtc cttcacgcag cgcagccagt gcattttcaa
    13321 cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg attcatggta aaaaaacctc
    13381 actaaaatta tggttaccag aatcagggca gtcttaggag tggcggcata tagccaaaat
    13441 aacgtgagcg ggtccatgcc cgacagaatc gttactctct cccatccgga ctctaaccgt
    13501 cggccccgga attacaccgg atctgctgtc ctttgagttc gcacccaaag cgctcgcggg
    13561 ctttcaactg agttgattta ccgccggtgg ggaatttcgc cccgccctga gaataagcgg
    13621 attcactata acgctaatga ttagcggcag caacgcatag cttcacataa ttctggttta
    13681 tgacttaccc ttatcgcact acaatggcac tcaacaccta tcagtacagg gaaactacaa
    13741 tgattgaccc gaaaaaaatt gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag
    13801 gaatcaggga gttcggggaa gatgtggaga aaaaaatccg ccaaacccta caagcgcagc
    13861 tgacgcgtct cgatctggtg agccgcgaag aattcgacgt acagacgcaa gtcctgttac
    13921 ggactcgtga aaaactggcg ttactggagc agcgcatcag tgaactggag gcgcgcaata
    13981 attcggtagc agatcttcaa tctcctccgg ctatcccgcc tattgataaa gcagaataaa
    14041 gcaacacaaa aacgggccag acggcccgta atatcagtgt tctaattcaa gttctctggt
    14101 ttgcatcatc tgttggcaat accaggcata acgtgcgcaa aaccaggttc tatgttcttc
    14161 atccatgtta ccagtaacgg ccagaatatc gaccgggcga ccttctgcgt gcattctggc
    14221 aaaactacgc gccaggaaat cgaaattatt taaagaatta agactatgat ccataagcac
    14281 atcctccggt tatcgcatac cttttaaggc catatgcttt ttctgatttc agtattcccc
    14341 aatcagtgta gtgcctttaa acataacgtg cgtaaatata ttgtcctcga tcacactttt
    14401 tagtgaaaaa taaaaaacca tat
//
LOCUS       DAZFFG010000114        14316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFFG010000114 DAZFFG010000000
VERSION     DAZFFG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.114"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            194..1765
                     /gene="garD"
                     /locus_tag="ACHJ7J_003909"
     CDS             194..1765
                     /gene="garD"
                     /locus_tag="ACHJ7J_003909"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO5002085.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFRSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTQRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1915..2250
                     /gene="prlF"
                     /locus_tag="ACHJ7J_003910"
     CDS             1915..2250
                     /gene="prlF"
                     /locus_tag="ACHJ7J_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO5002086.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2250..2714
                     /gene="yhaV"
                     /locus_tag="ACHJ7J_003911"
     CDS             2250..2714
                     /gene="yhaV"
                     /locus_tag="ACHJ7J_003911"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO5002087.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTQETEENH"
     gene            complement(2769..3578)
                     /gene="agaR"
                     /locus_tag="ACHJ7J_003912"
     CDS             complement(2769..3578)
                     /gene="agaR"
                     /locus_tag="ACHJ7J_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO5002088.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3827..5107
                     /gene="kbaZ"
                     /locus_tag="ACHJ7J_003913"
     CDS             3827..5107
                     /gene="kbaZ"
                     /locus_tag="ACHJ7J_003913"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO5002089.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5130..5603
                     /gene="agaV"
                     /locus_tag="ACHJ7J_003914"
     CDS             5130..5603
                     /gene="agaV"
                     /locus_tag="ACHJ7J_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO5002090.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVGCFVQGVPTEPAVDLFKLL"
     gene            5614..6393
                     /gene="agaW"
                     /locus_tag="ACHJ7J_003915"
     CDS             5614..6393
                     /gene="agaW"
                     /locus_tag="ACHJ7J_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO5002091.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6383..7261
                     /gene="agaE"
                     /locus_tag="ACHJ7J_003916"
     CDS             6383..7261
                     /gene="agaE"
                     /locus_tag="ACHJ7J_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO5002092.1"
                     /translation="MASNQTTLPNISENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVETYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTMTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7279..7713
                     /gene="agaF"
                     /locus_tag="ACHJ7J_003917"
     CDS             7279..7713
                     /gene="agaF"
                     /locus_tag="ACHJ7J_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO5002093.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            7710..8843
                     /gene="nagA"
                     /locus_tag="ACHJ7J_003918"
     CDS             7710..8843
                     /gene="nagA"
                     /locus_tag="ACHJ7J_003918"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5002094.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDASDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9194..10348
                     /locus_tag="ACHJ7J_003919"
     CDS             9194..10348
                     /locus_tag="ACHJ7J_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO5002095.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10361..11221
                     /gene="kbaY"
                     /locus_tag="ACHJ7J_003920"
     CDS             10361..11221
                     /gene="kbaY"
                     /locus_tag="ACHJ7J_003920"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO5002096.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11388..11864
                     /gene="agaB"
                     /locus_tag="ACHJ7J_003921"
     CDS             11388..11864
                     /gene="agaB"
                     /locus_tag="ACHJ7J_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO5002097.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11903..12706
                     /gene="agaC"
                     /locus_tag="ACHJ7J_003922"
     CDS             11903..12706
                     /gene="agaC"
                     /locus_tag="ACHJ7J_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO5002098.1"
                     /translation="MHEIILLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12696..13487
                     /gene="agaD"
                     /locus_tag="ACHJ7J_003923"
     CDS             12696..13487
                     /gene="agaD"
                     /locus_tag="ACHJ7J_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO5002099.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIALSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFSIL"
     gene            13488..14243
                     /locus_tag="ACHJ7J_003924"
     CDS             13488..14243
                     /locus_tag="ACHJ7J_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO5002100.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQHVDVSQLTVVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTSIPASFLWLHSNFICLINT"
BASE COUNT         3465 a         3612 c         3730 g         3509 t
ORIGIN      
        1 cataaggttg cgcgctaaag cacagatttg cgctttacct tgccgggcgg cactgcaatc
       61 cctgaaatga ttgacattga tcacatttct gcgtttaaac tcctgacatt cttatttcac
      121 ccaatgaagt catttatttt taaatgagac caggtcctca ttttaataac ccctggctgg
      181 agaatattgc acaatggcca acatcgaaat cagacaagaa acgccaactg cgttttatat
      241 aaaagttcac gacacagata atgtggcaat tattgttaat gataatggcc tgaaagcagg
      301 aacgcgtttt ccggatgggc tggaattaat tgaacatatt ccccaggggc ataaagtcgc
      361 attgctggac attccggcta atggtgaaat tattcgttat ggcgaagtga ttggttacgc
      421 cgtgcgcgca atcccacgcg gaagctggat cgacgaatca atggttgtac taccggaagc
      481 gccgccgcta cacacgctgc cactggcaac caaagtcccg gaacccttac cgccgctgga
      541 aggatacacc tttgagggct atcgcaatgc cgatggcagc gtgggcacca aaaacctgct
      601 cggcatcacc accagcgtcc actgtgtggc aggcgtggtg gactacgtag taaaaatcat
      661 tgaacgcgat ctgctaccga aatacccgaa cgtcgatggc gtggtagggc tgaatcattt
      721 gtacggttgt ggcgtggcga ttaacgcacc ggcggcagtt gttcctattc gtaccattca
      781 caatatttcg ctgaacccta actttggcgg cgaagtaatg gtgattggcc tgggttgtga
      841 aaagttgcag cctgagcgcc tgctgactgg aacggatgat gtgcaagcta ttccagtaga
      901 aagcgccagc attgtcagtt tgcaggatga aaagcatgtc ggttttcggt ccatggtcga
      961 ggatattttg caggtcgccg aacgccatct acaaaaactg aatcaacgtc agcgagaaac
     1021 ctgtccggct tcagaactgg ttgtcggcat gcagtgcggt ggcagcgatg ccttttctgg
     1081 cgtaacggca aacccggcgg ttgggtatgc gtctgatcta ctggtgcgct gcggcgcaac
     1141 ggtgatgttc tcagaagtca cggaagtgcg tgacgcgatc catctgctga caccacgcgc
     1201 agtgaacgaa gaggtcggta aacggctgct ggaggagatg gagtggtacg ataactatct
     1261 caatatggga aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct
     1321 ggcaaacgtg gtagagaagg cactcggctc cattgctaaa tcgggtaaaa gcgcaattgt
     1381 tgaagtgctg tcgcccggtc aacgcccgac tcaacgcgga ttaatttacg ccgcgacgcc
     1441 agccagcgat tttgtctgtg gcacgcaaca ggtggcttcg ggtatcacag tgcaagtgtt
     1501 tacgaccggt cgtggtacgc cgtacggcct gatggcggta cccgtcatta aaatggcgac
     1561 ccgcaccgag ctggcgaacc gctggtttga tttaatggat attaacgcgg gcactatcgc
     1621 caccggcgaa gaaaccattg aagaggtggg ctggaagttg ttccacttta ttctcgacgt
     1681 cgccagcggg aagaagaaaa ccttctcgga tcaatgggga ctgcataacc agctggcggt
     1741 gtttaacccg gcaccggtga cctgatttct tttgaaacgt ccctcgcaaa acatggcctt
     1801 agtgccatgt tttttattgt ttaaagcccc cacgtccatt aataatgcat ttgcattacc
     1861 ttagttcaag cttataattt gagcagaaaa caggctgtaa aaggacagtg aatcatgccc
     1921 gctaatgctc gctctcacgc tgtactgacc actgaatcaa aggtcacgat acgcggacaa
     1981 acaactatcc ccgcgccagt gcgtgaggcc ttaaaactga agccaggcca ggacagcatt
     2041 cattacgaaa ttctgcctgg tgggcaagta tttatgtgcc gactgggaga tgaacaggag
     2101 gatcatacta tgaatgcatt tttgcgtttt ctggatgcag atatccagaa caacccgcaa
     2161 aaaactcgtc cattcaacat tcaacaagga aagaaacttg tcgctggcat ggacgtcaac
     2221 attgatgatg agattggcga cgacgaataa tggattttcc acaaagggtt aatggttggg
     2281 cgctatatgc tcatccctgt tttcaggaaa cctacgatgc tttagttgcc gaagtcgagg
     2341 cattaaaggg aaaagatcct gaaaattatc agagaaaagc tgccacaaag ttattggcgg
     2401 tggtccataa agtgattgag gagcatatca cggtcaatcc atcatcaccg gcattccgtc
     2461 atggcaagtc gttaggctct gggaaaaata aagactggtc acgggtaaaa tttggtgctg
     2521 gtcgctatcg tctcttcttt cgttatagtg aaaaagagaa agtcatcatt ctgggatgga
     2581 tgaacgatga aaacacgctg cgcacctacg gtaaaaaaac agatgcctat accgtattca
     2641 gtaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc caagagacag
     2701 aagaaaacca ttgatggtat tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
     2761 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
     2821 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
     2881 ttatgtaaac tggaacgatt gaatttactg gaatcggtga ccacgatgat ccgttccgca
     2941 acttcgcaca tccgacggtt taaacgggct tcatcttcat tatgcgtgct gacgccgcgc
     3001 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
     3061 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
     3121 agcagctcaa cgccttccgc ttccagcaac gcattagcca cgttcatacc gttggtcatc
     3181 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
     3241 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
     3301 ttcattgcgg tgttcagcgc gcttttatct tccactgatg gctcgactga cggcgttgtg
     3361 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
     3421 gccagatcgt tgcggatcgt cacggtagat acgccataca atgccgacag atcgttaacc
     3481 tgcacactcc cttgctgtcg cagacgctga atgatctgtt ctcgtcgctc gctggtgcct
     3541 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     3601 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     3661 cccattgacg caagctactc tcgtttcagt gactttcatt atgtttcttt tgtgaatcag
     3721 atcagaaaac tattatcttt cgttttattt ttatctcacc atgacgcagt atcaactgaa
     3781 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     3841 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     3901 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     3961 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     4021 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     4081 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccgg ccgctcaggc
     4141 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     4201 ccttgattgc agcatgtcgt gtcaggacga tccgattccc ttaactgatg acatcgtggc
     4261 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcttgaac actttggcga
     4321 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcggcg cgcatgaaac
     4381 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     4441 tcacgccttt gaaaagcaag gtttgaatgc catctggcca cgcatcattg ccctggtggt
     4501 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     4561 cgccttaagc cagatggtcg aaagctacga aacgctgatt ttcgaagcgc actctaccga
     4621 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgac cactttgcca ttctgaaagt
     4681 tggcccggcg ctgaccttcg ccctgcgtga agctctgttc tctctggcag cgattgaaga
     4741 agaactggtg ccagcaaaag cctgttctgg tctgcgtcag gtgctggaaa acgtgatgct
     4801 cgaccgcccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     4861 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     4921 cgctttcgct catctggtac gtaatctggc ggattcacca attccgctgc cgctgatcag
     4981 ccagtatctg ccgctgcagt acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     5041 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     5101 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     5161 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     5221 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     5281 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     5341 atcgtgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     5401 cgctggtgaa aggaggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     5461 atggcaagca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     5521 acgacctgaa agccgctggg gtgggatgct tcgttcaggg cgtcccgaca gagcctgctg
     5581 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     5641 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggctt aacccatatg
     5701 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccggt
     5761 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     5821 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     5881 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     5941 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     6001 aacgccgata ctcgcggcat tgaacgcgta aactatctgg cactgctggc actcggcact
     6061 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     6121 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     6181 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     6241 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgcc
     6301 gcgctggcga tggcgctgat cgatctgctg cgtaaatctc ctgaaccgac tcaacctgcg
     6361 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acatcagtga
     6421 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     6481 cgcggagctg acgaaaaaag atatcaatcg cgtcgcctgg cgttccatgc tgttacaagc
     6541 gtcttttaac tacgaacgta tgcaggcttc cggctggttg tacggtctgc tgcctgctct
     6601 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     6661 cttcaatacc catccgtttc tggtgacatt tgttatcggc attatccttg cgatggagcg
     6721 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgctcgg
     6781 cgggattggc gatgcaatgt tctggctaac gctactgccg atttgtggcg ggattggagc
     6841 cagtctggca ttgcaaggct ccattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     6901 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     6961 gattccactc attaaagcaa ataccaaaaa agtcggccat gctgcgtcta tcgttgggat
     7021 gacggtaatc ggtgcgctgg tggaaaccta tgttcgttta agcaccacgc tggaaatcac
     7081 agcgggcgac gcagtggtta agttacaggc tgatgttatc gacaaactga tgcctgcctt
     7141 cttaccgttg gtctacacca tgaccatgtt ttggttggta cgccgcggct ggagtccgct
     7201 gcgtctgatt gcagttaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     7261 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagc
     7321 ggcatggaaa aagcaatgaa gcaaattctc ggtgagcaat cgcagtttat cgccatcgat
     7381 tttccggaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     7441 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     7501 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgatcaccgg caccaatttg
     7561 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga attccgtgta
     7621 caggcgctgg agtgtggaca tcgtgggctg accagtctgg tggacgagtt aggccgctgc
     7681 catgaagaat atccggtcga ggaagggata tgacacacgt tctgcgcgcc agaaggctgc
     7741 tgactgaaga gggatggctc gatgaccatc agttgcgtat tgctgacggt gtcatcgcag
     7801 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     7861 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgtcggatg
     7921 tactcgacaa gctggcaatg cacaaggcac gtgaaggtgt cggcagttgg ttaccgacca
     7981 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     8041 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     8101 cgcagaataa aggcgcgcat ccgccggaat tgtttcgcga gcttgaaatt gccgagctgg
     8161 atcaattgat tgccgtttct cagcacacct tacgcgtggt agcgctggca ccggaaaaag
     8221 aaggggcatt gcaggccatc cgccatctta aacagcaaaa tgtaagggtg atgctggggc
     8281 atagcgcggc gacctggcaa caaacccgcg ccgcgtttga tgccggtgcc gacggcctgg
     8341 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     8401 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     8461 cggcgatgtc gctgtgttgt tgctgtgcga aagagagaat cgtgctgatc accgacgcga
     8521 tgcaagcagc cgggatgccg gatggtcgct atacgttatg tggcgaagaa gtgcagatgc
     8581 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgctg tctgttgatg
     8641 cggcagtgcg caacatggtc gagttgacgg gcgtaacgcc tgcggaagcc attcatatgg
     8701 catcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     8761 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     8821 agggtcaatt agcttcgttt tgatagtttg ctcctttatt gggccttcac ttcccccgta
     8881 aggcctttct ttttctttct ttttgatctg tgcagcggtg tcggatgcga cgctaacgcg
     8941 tcttatccga cctacgatac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     9001 caattcttta atcttccctt gtcttttctt tcaatgatca cagatttcgt tttatttctt
     9061 ttttctccat tgaactttcg gtttcttttc tatagatttt aatcaacgaa agacatcacc
     9121 aagtgaaatg aaacgaaagg caagcgaaag cgacaacgcc cgacgtcaag ttcatcagac
     9181 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg gtacatggac
     9241 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcgctcacca acatcgacgc
     9301 gctacattcc gcgcttaata acttccttga accgttactg cgcaaagaga atctgcggat
     9361 catcctgacc ggagccggaa cctcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     9421 cagccatacc ggtaaaaact tcagcgccgt accaaccacc gatctggtca ctaatccgat
     9481 ggactacctg aacccagccc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     9541 cccggaaagc gtcgcagccg tggaactggc aaatcaattt gtaccggaat gctatcacct
     9601 gccgattacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     9661 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     9721 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     9781 cttccgcgac gtggcggatc gttgccaggc gatcctgacc tcactgggcg atttcagcga
     9841 aggtgtgttt ggttacgcac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     9901 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg acggcgggta aactggcggc
     9961 cttttatgat tctccaaccg gattccgtca tggaccaaaa tcgctggtcg atgacgaaac
    10021 gctggtggtg gtatttgtct ccagccaccc ttacacccgt cagtatgatc ttgatctgct
    10081 ggctgaactt cgccgtgaca accaggcaat gcgtgtaatc gccatcgccg cggaaagcag
    10141 cgacatcgtc gctgccggtc cacatatcat cctgccaccg tcacgtcact ttatcgacgt
    10201 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
    10261 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
    10321 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccactaa
    10381 atatctgtta caggacgccc aggccaatgg ctacgcggtg cctgccttta acattcataa
    10441 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
    10501 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgcag
    10561 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
    10621 ggatgatatt cgccgtaaag tccacgcagg tgtgcgcagt gcaatgatcg acggcagcca
    10681 cttcccgttt gccgagaacg tgaagctggt gaaatcggtt gttgacttct gccactcaca
    10741 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
    10801 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaacgctttg tcgaactgac
    10861 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
    10921 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
    10981 acatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
    11041 aaaagtgaac gttgccacag aattaaaaat agccttcgcc ggcgcggtta aagcctggtt
    11101 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
    11161 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
    11221 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtgcctt
    11281 aatggaaaag gagatgacta aaccttaata aatacgtcag tacaatatcg caacaataat
    11341 atatttaaaa aaattatatt attcaacttt atggtgagga ttacacaatg agcagtccaa
    11401 atattctctt aacccgtatt gataaccgtc tggttcacgg tcaggttggc gtgacctgga
    11461 catccaccat cggtgcgaat ctgctggtag tcgtggatga tgttgtcgct aacgatgata
    11521 ttcaacagaa attaatgggt attaccgcag aaacctacgg ctttggcatt cgtttcttta
    11581 ctatcgaaaa aaccattaac gtcatcggca aagctgcacc gcatcagaag atcttcctga
    11641 tttgccgtac gccacaaacg gtacgtaaat tggtagaagg tggcattgac ctgaaagatg
    11701 tcaacgtcgg caatatgcat ttctcggaag ggaaaaagca aatcagcagt aaagtttatg
    11761 tcgatgacca ggatctcacg gacttacgtt ttattaaaca acgtggcgtg aatgttttca
    11821 ttcaggacgt ccctggcgat caaaaagaac aaatccctga ctaaatctaa aatcgcctta
    11881 atattggttt gaggtaataa aaatgcatga aataatccta cttcagggat tatccctggc
    11941 ggcgttagtt ttcgttctgg ggattgattt ttggctggaa gccttatttt tattccgccc
    12001 gataatcgtt tgtaccctaa ctggcgctat tctcggtgat attcagactg gcttaattac
    12061 cggtggtctg acagagttgg ctttcgccgg attaacccct gcaggtggtg ttcagccccc
    12121 caaccctatt atggcgggtc tgatgaccac cgtcattgca tggtctacgg gcgttgatgc
    12181 caaaacggca attggtcttg gcctaccgtt tagtttgtta atgcagtacg tcattctgtt
    12241 cttctattct gctttctcat tatttatgac caaagccgat aaatgcgcga aagaagcgga
    12301 tacggcagcg ttttcccgac ttaactggac aacgatgctc atcgtcgctt cagcgtatgc
    12361 ggtgattgct ttcctctgta cttacctggc acaaggggcg atgcaggcgc tggtgaaagc
    12421 gatgcccgcc tggctgaccc acggctttga agtggctggc ggtattctgc ctgccgttgg
    12481 ttttggcttg ctgctgcgcg tgatgttcaa agcacaatat atcccttacc tgatcgccgg
    12541 cttcctgttt gtttgctaca tccaggtcag caacctgttg ccggttgccg tactgggcgc
    12601 aggctttgcg gtgtatgagt ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc
    12661 cgttgccagt aaaaatgaag aagaggacta cagcaatggg atctgaaatc agtaaaaaag
    12721 atatcacccg tctgggcttt cgttcgtcgc tgctgcaagc gagctttaac tacgaaagga
    12781 tgcaggcggg cggttttacc tgggcgatgt tgccgatcct gaaaaagatt tataaggatg
    12841 acaaaccggg cctaagcgca gcgatgaaag ataacctcga atttattaat acccacccga
    12901 atctggtcgg attcctgatg gggttattaa tttcgatgga agaaaaagga gaaaaccgcg
    12961 acaccattaa aggcctcaaa gtggcactgt ttggcccaat cgccgggatt ggcgatgcga
    13021 ttttctggtt tactttgttg ccgattatgg cgggaatttg ctcatcattt gccagccagg
    13081 gaaacctgct ggggccgatt ctatttttcg ccgtttacct gcttatcttt ttcctgcgcg
    13141 tcggctggac ccacgtcggt tattcagtcg gcgtgaaggc gatcgataaa gtgcgagaga
    13201 actcgcagat gattgctctc tcggcaacca tcctcgggat cacggtaatc ggcgggctga
    13261 tcgcttcgta tgtgcatatt aacgtggtga catcgtttgc catcgacagt acccacagcg
    13321 tcgcgctgca acaggatttc ttcgataaag tcttcccgaa cattttaccg atggcctaca
    13381 ccctgctgat gtattacttc ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg
    13441 tgacttttgt gctctctatt gtttgttccg cattcagcat tttgtaaatg gaacgaggca
    13501 ctgcgtctgg cggtgcctct ttacttaagg aatttcatcc tgtgcaaacc cttcagcaag
    13561 ttgaaaacta tacggcgtta agtgaacgtg ccagcgaata tttattggcc gtgatccgta
    13621 gcaaaccgga tgccgtgatt tgcctggcga ccggagccac gccattactg acgtatcatt
    13681 atctggtaga aaaaatccac cagcagcacg ttgatgtcag ccagctcacc gtcgtcaaac
    13741 tcgatgaatg ggtggatctg ccattaacga tgcctggtac ctgcgaaact ttcctgcaac
    13801 agcatatcgt gcagccgctg gggctacgtg aagaccagct catcagcttt cgctccgaag
    13861 agataaatga gacagagtgc gaacgggtaa cgaacctgat tgcgcgcaaa ggcggtctgg
    13921 atttatgcgt tctcggattg gggaaaaacg gtcatcttgg gctgaacgaa ccgggggaaa
    13981 gcctacaacc ggcctgccat atcagtcaac ttgatgccag aacacagcaa catgagatgt
    14041 taaaaaccgc gggtcgcccc gtgactcgtg ggatcacctt aggcctgaag gatattctca
    14101 atgcccgcga ggttttgtta ctggtgactg gcgaaggaaa gcaggatgcg acagaacgtt
    14161 ttctcacggc taaagtctct acctctatcc cggcttcgtt tttgtggctg cacagtaact
    14221 ttatttgttt aattaatacc taaccagatg aaaaatttgc tcaaaatgtt taaattaact
    14281 tattcaacag tcacgcatta tattaaataa catttg
//
LOCUS       DAZFFG010000115        14269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFFG010000115 DAZFFG010000000
VERSION     DAZFFG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.115"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            135..617
                     /locus_tag="ACHJ7J_003925"
     CDS             135..617
                     /locus_tag="ACHJ7J_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO5002101.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDSKKTAKKNEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGLISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNTAGPDDVDPLAELLKARD
                     "
     gene            617..1645
                     /locus_tag="ACHJ7J_003926"
                     /pseudo
     CDS             617..1645
                     /locus_tag="ACHJ7J_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            1816..3024
                     /locus_tag="ACHJ7J_003927"
     CDS             1816..3024
                     /locus_tag="ACHJ7J_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5002102.1"
                     /translation="MALNKLSDKKLRSLLGRRSERQETIADGNGLSVRVSKYGCVSFV
                     FFYRLAGRGTAPIWLTLGKYPDLSLKSAREMRDQCRTWLAEGRDPRIQIKIEREATLQ
                     PVTVREALEYWLDNYAMDKRRGAEHIRQCFGKHIYPVIGHVPLSDCSISMWIKCFDKI
                     KKVAPVQAGALLRISKQALKFCRVRKYAISHEIDDLEVCDVGKKSARRSRVLTDDEIR
                     DLWRSINTDYDNHELSYENRIILRFLVVFGCRLSEVLLSSWVEWDFDKKLWRVPADHS
                     KNGREIIRPIPDGMFNWLVTLKKITGNKENVIGFDMRQCTASVTICKTWKRMKHSEKW
                     TAHDMRRVFATKLSDHGFEHNVVEQLLGHTLGGVAGVYNRSQYMDRKKEAMNWWYDYL
                     NKLISGDGND"
     gene            3017..3238
                     /locus_tag="ACHJ7J_003928"
     CDS             3017..3238
                     /locus_tag="ACHJ7J_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002103.1"
                     /translation="MIEIVTREELENDKTIDRMIKEDECAWLTALGRRHRALLEKEGK
                     FPRKICIGPQTKVWRLSEVLEWVKGEWKP"
     gene            3302..3580
                     /locus_tag="ACHJ7J_003929"
     CDS             3302..3580
                     /locus_tag="ACHJ7J_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002104.1"
                     /translation="MQKFIYPTPEERIQILKEHGEPYDRRIREHECANRTGLSRSRRW
                     VLEQEGAFPARAHLGKVSVSWLLSDVLWWVMHPPGVKEVNSPYKNANK"
     gene            3638..4231
                     /locus_tag="ACHJ7J_003930"
     CDS             3638..4231
                     /locus_tag="ACHJ7J_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO5002105.1"
                     /translation="MNKNIAATGKGDARHVKKFCDIRDLVVLRFDSVNVRVVYLNGDP
                     WFVGADVCAALEISNVTDAVSVLDSDEVMTLALTEGHSGKRGGARSWNVVSESGFYKL
                     IARSRKAVNPGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRRKEQYQISASE
                     KGRELQACKHKKRELEEEEKRLIREYQPEFYFGERIQ"
     gene            4376..4618
                     /locus_tag="ACHJ7J_003931"
                     /pseudo
     CDS             4376..4618
                     /locus_tag="ACHJ7J_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="icd-like protein"
     gene            complement(4777..4959)
                     /locus_tag="ACHJ7J_003932"
     CDS             complement(4777..4959)
                     /locus_tag="ACHJ7J_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002106.1"
                     /translation="MTNKESTNTPSQKTKREHENMALEHESERFAPCAFALEIFLKEY
                     RKTRPLQVWKRNEPKG"
     gene            complement(5017..5322)
                     /locus_tag="ACHJ7J_003933"
     CDS             complement(5017..5322)
                     /locus_tag="ACHJ7J_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5002107.1"
                     /translation="MATGNINSRSQMKNIRFPHDVIEEMENSKTEGETIAAFVITAVR
                     GEIARRQAEGSGENPLVSSLDALAQVEKIGVKAAEEIGQLVTVAREELQRRKAKESE"
     gene            5519..5725
                     /locus_tag="ACHJ7J_003934"
     CDS             5519..5725
                     /locus_tag="ACHJ7J_003934"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5002108.1"
                     /translation="MRNHTIHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RLQSPDGCVMVFAARIRQEVRHVQ"
     gene            5715..5954
                     /locus_tag="ACHJ7J_003935"
     CDS             5715..5954
                     /locus_tag="ACHJ7J_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002109.1"
                     /translation="MCNKNTPDAAAEAIRTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGYA"
     gene            5947..6198
                     /locus_tag="ACHJ7J_003936"
     CDS             5947..6198
                     /locus_tag="ACHJ7J_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002110.1"
                     /translation="MRDDRFNSLKQEFSGVSYDVGDALLSISEIMRVAFFFLCTDEHR
                     DTGLNILDIASDYADYVAEFNLRENQLLREQNKTPSTGE"
     gene            6204..6518
                     /locus_tag="ACHJ7J_003937"
     CDS             6204..6518
                     /locus_tag="ACHJ7J_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5002111.1"
                     /translation="MKLKYSGLTASGPARPEIRNGDIYRDKYGSQITVKTVSAYRVTY
                     IREGYAHPCVSSHMRFEREFTLVSKAPPADLSDIDRIMRITGMERIKAVREIIRERGK
                     AK"
     gene            6515..8644
                     /locus_tag="ACHJ7J_003938"
     CDS             6515..8644
                     /locus_tag="ACHJ7J_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO5002112.1"
                     /translation="MKLAPNVKLLPKDKGEDAVIFAGDDAYSHAEHYMQGGEARKRGD
                     KIPPVYLGRRDLGNLENLRIVDDGRSRAIVRRAGKLDDKLALQIETLLAVAGVKEACF
                     CDENGDLLEDWTPQLARLKDEYERGESLVLPLKKKATTSQGDDELKPRVESRADGVFW
                     VTPKVDKQSGEIIRPETWLCSPLELLGTGTIGKEYYRVMRWKKPANHEVITMAVPCGG
                     IGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGNHEEWQLSTTTGWHFGAYIMPDG
                     SVIGDCEKPVLFTGKTAAVNGYSVAGTAEGWRDTVARLAGGNPSMMLGVATSLAAPLI
                     GLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEAHNDG
                     LLPLDEIGQAGNAREVSTSAYTLFNGSGKLQGAKDGGNREIKHWRTVAISTGEMDVET
                     FLKSEGIKVKAGQLVRLLNVPMEKATKFHEYSNGKEHADALKDAWTANHGAAGREWVK
                     WLANHQQEAKDTVRECRERWRNLIPESYGEQVHRVGERFAILEAALVLSGHVTGWVVQ
                     ECRDAIQHNFNAWVKEFGTGNREHKQIIEQAEAFLAAYGMSRFAPVDYDPASLPISEL
                     YGYRESDGRYDKPVLFYVLPQPFISHVANGFNKDAVAKALHEAGMLKKPTSGKGWQIR
                     TPRLKHLKGARLRVYGLLLAQDDAESD"
     gene            9003..9353
                     /locus_tag="ACHJ7J_003939"
     CDS             9003..9353
                     /locus_tag="ACHJ7J_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002113.1"
                     /translation="MFIEAIPKSISPAIPGWWARFKDDDCGEWWSPVAAWAICDVFYS
                     GDSETYSEILPVLTGDNGMTPHHSAEGYCECLYLPDTKFILSDEPGTLAWHPADNTAE
                     QVTTINVKTGEQTA"
     gene            9350..9772
                     /locus_tag="ACHJ7J_003940"
     CDS             9350..9772
                     /locus_tag="ACHJ7J_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5002114.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKYADFLAKHQKGDVASVSGAMQVSQWTGQNGETRQGYQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            9790..10038
                     /locus_tag="ACHJ7J_003941"
     CDS             9790..10038
                     /locus_tag="ACHJ7J_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002115.1"
                     /translation="MPVTFEEVQQHKKFHGFDDLETTTAKKYRRLLSSDALFVVDHHD
                     FLRSSLTGEIFATNREQVEAMIEYLWKIRRRMRDPVKQ"
     gene            10313..11458
                     /locus_tag="ACHJ7J_003942"
     CDS             10313..11458
                     /locus_tag="ACHJ7J_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5002116.1"
                     /translation="MKKLIELRQQKTALKNQMRSLLEKADSENRSLTDDEGKQFDELR
                     AKADSLDTEISRLEAVADEERNQPGVSVEEKITKDELRSYILTGETRNLSGSVPADGG
                     YTVIPELNREIMRQLSDESVMRKICTVKTIHSNEFKQLVSAGGAVVEHGEEGTTRNQT
                     ATPKLNEVSIRLYPIYAYPKTTQEIIDFSEVDIMSWLSSEIGDTFVDTEETDLVSGDG
                     EKKAKGFLAFPRTADNDKTRPFGTLQTKKVTGSISADMLIDLKFTLRNKYRKKAVWVM
                     NSNTAASAQKLKNATGDYIWRDRLQAGDPDSLLGLPVEYLEFMPDGVIAVGDFKRGYF
                     IVDHETGTRTRPDFSEPGFINIYTQKYLGGGVVDSNAIKILEIQAGK"
     gene            11508..12068
                     /locus_tag="ACHJ7J_003943"
     CDS             11508..12068
                     /locus_tag="ACHJ7J_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5002117.1"
                     /translation="MKNTDFEIRTSELTASDKKLVGYAVRWNSLSEIIWDEFREQFMP
                     GAFADYLAAGNDVRCLYEHDYTRLLGRTKSGTLVLTEDNTGLRFELTPPETQPGKDVL
                     TLVERGDITGMSFGFRALCEEWNIAQKPYLRTVTAAELREITITSMPAYPESGVEIAR
                     RSLFAQHPELRPTENNRHRWAELAGL"
     gene            12070..13284
                     /locus_tag="ACHJ7J_003944"
     CDS             12070..13284
                     /locus_tag="ACHJ7J_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5002118.1"
                     /translation="MWWPFSRKKSDLRNLSIDDFLALSGVPNTGSGEYVSAGTAESLP
                     AVMNAVSVIAEAVATMPCYLYLVRNDKGREAREWLDSHPVDILLNEQPNSCQTPYQFK
                     RTMMRHCLLNGNAYAVIEWGRDGQPKSLHPYAPGCVVPERTGAHKYRYTITEPYTGTV
                     RTYLQEEVLHLRYASDDGFLGRSPVTICREALGLGLAQQRHGASIMKDGMMAAGIITS
                     GEWLDGVKGKQALDALERYKGAKNAGKTPILEGGMDYKQLGMSNQDAEWLASRRFSIE
                     DIARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKAALLVASPVP
                     GTRYLVEFDSADLLRATPTERYATYEKGIKNGIMNPNEAREREGMPPREGGDEFSQAW
                     KQTVEIKGEKDE"
     gene            13277..13576
                     /locus_tag="ACHJ7J_003945"
     CDS             13277..13576
                     /locus_tag="ACHJ7J_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5002119.1"
                     /translation="MSEEKITPDEVRAHLRLDDFSGEGELLKVYTDAALEACQKHIGK
                     RFEDGLEFTPAIRVGCLMYIAFLYENREAVSPVEQSELPMAISALWSVYRDVGVY"
     gene            13576..14016
                     /locus_tag="ACHJ7J_003946"
     CDS             13576..14016
                     /locus_tag="ACHJ7J_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5002120.1"
                     /translation="MPWQPLRRCTEPGCNKRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRALYLSKNPLCVRCLAKGIYTPALVVDHIIPINGGGDVLFWPEWNHQAL
                     CQTCHNRKTTREDPATKANRKAGMYREQEERAAHRNDWMYGDDD"
     gene            14000..14185
                     /locus_tag="ACHJ7J_003947"
     CDS             14000..14185
                     /locus_tag="ACHJ7J_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002121.1"
                     /translation="MAMMTEQEQNRLIRGLIRQRDLWKTQETGHKANKTGRTERTTAK
                     RLTDRDREVMECFRNRW"
BASE COUNT         3799 a         3167 c         3930 g         3373 t
ORIGIN      
        1 aagggggagg ggggggatca aatctctgca acccaggctg ctcagtaccg ccgcctgacc
       61 tttcctcaca tcgccgcagg ttcgaaaact tttttttgga aatgtgaaca aacgattgat
      121 aggtaaaact gattatgtcc ggacccccga aaaccccgcc acgcctgcat ttgatacgag
      181 gcaacccctc aaagcgcccc gttaaagact caaaaaaaac cgctaaaaag aatgaaaaag
      241 gtctccctaa aattccgcag catttagggg cgcaggggaa gtactggttc aggcgaatgg
      301 cggaagagct gaatgcggaa gggctcattt ctcagcttga tgcgcgtgca ctcgagttac
      361 tggtggaagc ctacaccgaa taccggcatc actgcgaaac actcgatgtt gaggggtata
      421 cctaccgcac ggaaacgcag aacggtgatg tgctgattaa ggcgcacccc gcggcggcaa
      481 tgaaagcgga tgcctggaaa cggatccggg caatgcttgc agagtttggt atgtcaccgg
      541 caagccgggc taaagtaaat accgccggac cggatgatgt tgatccgctg gcggagcttt
      601 taaaagcgag agactgatgg caaaagtggc tgacgggatc cgctacgccg aacgtgttgt
      661 tgcaggagaa attgttgctg gcgaatttgt ccgtctggcc tgccagcgtt ttcttgatga
      721 tctgaagtac ggcgaagagc gggggattta tttcagtgaa ccccgtgcgc agcacatcct
      781 gaatttctac aaattcgtgc cccatgtgaa aggggcgctg gcaggccagc ccattgagtt
      841 gatggactgg catgttttta tcctcattaa tatttttggt tttgtcattc cactggtcaa
      901 tgaagagacc ggggaagttg tcatgcgcag cgatggcagc ggacgcccgg tgatggtgcg
      961 ccgtttccgg acagcgtaca acgaagtcgc ccgtaaaaac gcaaaatcaa ccctgtcatc
     1021 gggtatcggt ctgtatatga cgggggcaga tggtgaaggt ggtgctgagg tgtattcagc
     1081 cgcaaccacg cgtgaccagg ccagaattgt gtttgaagac gccaaaaata tggtcagaaa
     1141 agcccggtcg acactcgggc ggttgtttga tttcaacaag ctggcgattt accaggagca
     1201 gagtgcatca aaatttgaac cgctttcctc ggatgcaaac aacctggacg gtctgaacat
     1261 ccactgcgcc attattgatg agctgcatgc acataaaacc cgcgacgtgt gggacgttct
     1321 ggaaacggca accggtgccc gtctgcagtc cctgttattt ggtatcacca cggcgggctt
     1381 taacaaggaa gggatttgtt acgaacaacg cgattacgcc atcaaggtat tgcgaggcta
     1441 taacagcgac gtggagggcg cggtaaaaga cgactcctac tttgcgatta tttacacgct
     1501 cgatgaggga gatgatccat ttgatgaaac ggtctggcag aaagcgaatc cgggcctggg
     1561 catctgtaaa cgctgggatg atctgcgtcg tctggcgaaa aaagcgaagg agcaggtctc
     1621 ggcgcgggtg aattttttca cgaagaaagt aacatcacgc cgtaacaaca gtgcgacctg
     1681 tcctgatttt tttagtaacc aaatgaaaga aaaagatttt ttcttgttcg tcgtttttgt
     1741 tttttctgga aggttctggc tgttttcatc atttgtgtat tgcactgtgt attgcaaaaa
     1801 cgggttataa atcacatggc gctaaacaaa ctgagcgata aaaaacttcg ttccctgctt
     1861 gggcgcagga gtgagaggca ggaaaccatc gctgatggta acgggctttc ggtacgggtc
     1921 agtaaatacg gatgtgttag ttttgttttc ttttacagac tggcgggaag gggaaccgcg
     1981 cccatctggc tgacacttgg aaaatatcct gatctgagtc tcaaatcagc gagagagatg
     2041 cgcgatcagt gtcgaacctg gcttgcggag ggcagagatc cacggattca gataaaaatt
     2101 gaacgggaag ccaccttgca acctgttacc gttcgtgagg cacttgaata ctggcttgat
     2161 aattatgcaa tggataagcg taggggagca gaacatatca ggcagtgctt tggtaaacat
     2221 atttatccgg tgattggtca tgtaccactt agtgattgct ctatatctat gtggatcaag
     2281 tgttttgaca aaataaaaaa agtagcacct gtacaggccg gagctttgtt gcgtatatca
     2341 aaacaggcgc ttaaattttg tagggtaaga aaatacgcga ttagtcatga aattgatgat
     2401 cttgaggtct gtgatgtggg aaaaaagtct gcgcgaagaa gcagggtttt aacagatgat
     2461 gaaatcagag atttatggcg aagtattaat actgattatg acaatcacga attatcatat
     2521 gaaaaccgaa ttattttacg tttcctggta gtttttggtt gtcgactgtc agaagtatta
     2581 ctgtcgtcct gggtagagtg ggattttgat aagaaattat ggcgcgttcc cgctgatcat
     2641 agcaaaaatg gcagggaaat aatcagaccg attcctgatg gcatgtttaa ctggttagtt
     2701 acgttaaaaa aaataacagg taacaaagaa aatgtgattg ggtttgatat gcgtcagtgt
     2761 acggcaagcg taactatctg taagacatgg aaaaggatga aacactcgga aaaatggacg
     2821 gcgcatgata tgcgaagagt gtttgccaca aagctaagtg atcatggttt tgaacataat
     2881 gtggttgaac agttgcttgg gcacacatta ggcggtgttg ccggggttta taacagaagt
     2941 cagtatatgg acagaaaaaa agaagctatg aactggtggt acgactatct gaataagcta
     3001 attagtggtg acggtaatga ttgaaatagt tacgcgtgaa gaacttgaaa atgataaaac
     3061 aatcgacagg atgattaagg aagatgaatg tgcatggtta acagctcttg gcaggagaca
     3121 cagagcatta cttgaaaagg aaggaaaatt tccacggaaa atatgtattg gcccacaaac
     3181 aaaagtctgg cgcttatctg aggtgctgga atgggtaaag ggtgaatgga aaccctgaac
     3241 taaattaaat gagatataat caacccgtct tttggcgggt ttttttatag gtgatatttt
     3301 tatgcaaaaa tttatttatc ctacacccga agaacgcatt cagattctga aagaacatgg
     3361 cgaaccgtat gatcgccgta tacgcgaaca tgagtgtgcc aatcgtaccg ggctttcaag
     3421 aagcagacgt tgggtacttg aacaggaggg agcatttcct gctcgtgctc atttagggaa
     3481 agtgtctgtt tcctggttgc tctctgatgt gctctggtgg gttatgcatc cgccaggagt
     3541 aaaggaagta aacagcccat acaaaaacgc caataagtaa ttaccgacaa ccccgcacca
     3601 cgcaatgcgg ggttttttgt atgtgaggta gaaagtgatg aataaaaata ttgccgcgac
     3661 gggcaagggt gacgcacgtc atgtgaaaaa attctgtgat attcgtgatc tggtcgttct
     3721 gcgctttgat agtgtgaacg ttcgcgtggt gtatctgaac ggcgatccgt ggtttgttgg
     3781 cgctgatgtg tgtgcggcgc tggaaattag caacgttacc gatgctgttt cagttctgga
     3841 tagcgatgag gtaatgaccc tagctttaac cgagggtcat tcaggtaagc gtggtggtgc
     3901 cagaagttgg aatgttgtat cagaatcagg tttttacaag ttgatcgcca gaagtcgcaa
     3961 agctgttaat cctggcacgt tcgcccatcg tttcagtaat tgggtattca gaaatgtgat
     4021 accgggtatc agaaaaacgg gggcttatgg tatcccgtgg ggcgcattac aggatttttc
     4081 ccgccgcaaa gagcaatacc agataagtgc cagcgagaag gggagggagc tacaggcatg
     4141 taagcacaaa aagcgtgaac tggaggaaga agaaaaaagg ctgatacgtg aataccagcc
     4201 tgagttttac tttggtgagc gtattcagta accacacgcg gtgttgatta tacggtacat
     4261 cgtgttgacc gggaagctac ccaccagcaa ggcaaaatct tctgctaaaa aatgacatat
     4321 taccagtcgt ccggaaagca tgaaatttta caaaaatgga aaatgaagat ttttattgtg
     4381 ctggtgggta aaaacaaaaa gcgccccgtt accggagcgc ccttgcgaac aattaacctg
     4441 ctgcgaaaaa attggatcag tgcaggggaa ttatatcaac tgtgtgaaga agcgccacaa
     4501 ttgccggata acaggcaaaa caaaggccac ctgctacggt ggccattcga cacaagctac
     4561 acgttatccc caacgcatga gcataaccaa caatgccaca tttgcggctg gtgggcaaag
     4621 cgtaaccgga aaaaaattcc ggtggggcta ttcctgttct ttgtccttgc gtcgctggcg
     4681 atgtttgatc tcatgcagaa ttttttcagg taagaatcct gctgatttat tttctggtag
     4741 caatggtttg ttatccgtct gcttcctggt catacatcaa ccttttggtt cgttacgttt
     4801 ccatacctgc aatgggcgcg ttttcctgta ctcttttagg aatatctcaa gagcaaacgc
     4861 acacggtgcg aatctttctg attcatgctc gagcgccata ttttcgtgct ctcttttcgt
     4921 tttttgcgat ggtgtattgg ttgattcttt gttggtcatt gtgtgttcct gtaaagcaat
     4981 gcgccggagt tcctcacacc acggcgctga tagtgattat tctgattctt tggccttgcg
     5041 gcgctggagt tcttcacgcg cgacggtgac gagttgcccg atctcctcgg ctgctttgac
     5101 accgattttt tccacctgcg ccagtgcatc gagcgaagaa accaggggat tttctccgct
     5161 tccttctgcc tggcggcggg cgatttcacc gcgaacggct gtaatcacga aagcggctat
     5221 tgtttcccct tccgttttcg aattttccat ttcctcgatt acatcatggg ggaagcggat
     5281 atttttcatt tgcgatctgc tgttgatgtt tcctgttgcc atcagaatgt ccggttattt
     5341 tgaggtgtgt acacattaca ccaaaaggtg tgttgataaa acacttgaca ctgtagcaca
     5401 cttaaatcta atctgtatac acaccaatta ttgtagtgta tatacagcaa cgccccgcag
     5461 tggtggaaca cttgcagggc gtctaaccac caacgatagc aagattatcg aggtagctat
     5521 gagaaatcat accatacacc cgcaagggcg ggattcgcac aacctgaata aatacatctg
     5581 gcgttttatc gccctgagta cagcacaacc gcgcgtgatt accatcgagg ccaccagcga
     5641 acaggaagcc cgcctgcaat ccccggatgg ctgcgtgatg gtattcgccg cccgtattcg
     5701 tcaggaggtg cgccatgtgc aataaaaaca cgccggacgc agccgccgaa gctatcagaa
     5761 cgctaatgca cgcgctgatt gatatttctg ttatcgcaga cagggcgcat aagcacgcca
     5821 ccagtgaaac agaatatgcc ggggctttcg ttcctcattc gctggcggtt atgcaactta
     5881 gtgctgatat ggcgctgaat gaggccaaag ctatcctgat tgctgattgt gaaaatgggg
     5941 ggggttatgc gtgatgaccg ttttaattcc ctgaaacagg aattttccgg cgtgtcttat
     6001 gatgtgggtg atgcgctttt atcaatatct gaaattatgc gggtggcttt tttctttctt
     6061 tgcactgatg agcacaggga tacaggacta aatattcttg atatcgcctc tgattatgct
     6121 gattacgtgg ctgaatttaa tttaagagaa aaccagttat tacgcgaaca gaataaaacg
     6181 ccttccaccg gagaataaaa ataatgaaac ttaaatattc tggcttaact gccagcggcc
     6241 ccgctcggcc tgaaatccgt aacggcgata tttaccggga taaatacgga agtcagataa
     6301 cggtaaaaac ggtgagtgct tatcgggtga catatatccg cgaaggttat gcgcatccct
     6361 gcgtgtcgtc acatatgcgc tttgagagag agttcaccct agtaagcaaa gcaccaccag
     6421 cggacttaag cgacatcgac agaatcatgc gcattacagg tatggaacgc attaaagcag
     6481 tacgtgaaat tattcgtgag cggggaaagg caaaatgaaa ctggcaccaa acgtaaaact
     6541 gttaccgaaa gataaaggcg aggatgcagt gatttttgca ggggatgacg catacagcca
     6601 tgcagagcat tacatgcagg gcggagaagc cagaaagcgc ggcgacaaaa taccacctgt
     6661 ttatctcggg cggcgtgacc tgggtaacct ggaaaacctg cgtattgtgg atgatggtcg
     6721 gtcacgcgcc atcgtcagac gtgccggaaa actggatgac aaactggcgc tacagattga
     6781 aaccctgctg gcagtggcag gtgttaagga agcctgtttt tgtgacgaga acggcgattt
     6841 actggaggac tggacgccgc agcttgcccg gctgaaagac gaatacgagc gcggggaaag
     6901 tcttgtgttg cccttaaaga aaaaagccac cacaagccag ggtgatgatg aattaaagcc
     6961 ccgcgttgaa agccgcgccg atggtgtttt ctgggtaacg cccaaagtgg ataagcagtc
     7021 aggcgaaatt atccggcctg aaacgtggtt atgttccccg cttgaactac tgggaacggg
     7081 gacaatcggc aaagagtatt accgcgtgat gcgctggaaa aagccagcaa atcatgaagt
     7141 catcacaatg gcggttccgt gcggtggcat tggcgaccgt gacggctggc ggctactgaa
     7201 agaccacgga ctgaacgtaa caactaacgg caaatacaga gctatcctgg ctgactggat
     7261 gcagttaagc ggaaaccatg aggaatggca gttaagcaca acaacgggct ggcattttgg
     7321 cgcgtacatc atgccggacg gttcggtcat tggtgattgc gaaaagccag tcctgtttac
     7381 cggaaaaact gccgctgtta atggctattc cgtggcagga acggcggagg gctggcgcga
     7441 taccgttgcg cggttggctg gtggtaatcc gtccatgatg ctgggtgtgg caacgtcact
     7501 ggcagcgcct ttgattggcc ttgtgggagc tgacggcttc ggggtgcatc ttttcgaaca
     7561 gtcatcggca ggtaagacca ccacgcagaa catcgcatca agtttatggg gagagccgga
     7621 cgcgcagcgg ctgacctggt acggcacggc gttaggtatt gccaatgagg ccgaggccca
     7681 taatgacggg cttttaccac tggatgagat aggccaggcc ggaaatgcgc gggaagtgtc
     7741 aacgtctgct tatacgctgt ttaacggttc agggaaattg cagggggcga aggatggcgg
     7801 caacagggag atcaaacact ggcgaacggt ggcaatcagc accggagaaa tggacgttga
     7861 gacattcctc aaaagtgagg ggataaaagt taaggcgggg cagcttgtcc gattgcttaa
     7921 cgttccgatg gaaaaagcca cgaaatttca cgaatacagc aacggtaagg aacatgctga
     7981 cgcgttaaag gatgcctgga cagcaaatca cggggcggcg ggtcgtgagt gggttaaatg
     8041 gctggcaaac caccagcagg aggcaaagga cacggtaagg gaatgccgcg aacggtggcg
     8101 caacctgata ccggagagtt acggcgagca ggtccaccgc gtgggtgaac gtttcgccat
     8161 actggaggcc gcgcttgtgc tttccggtca tgtaactggc tgggtcgtgc aggaatgccg
     8221 cgacgcgata cagcataact ttaatgcctg ggtgaaggag ttcggcacgg gcaacaggga
     8281 gcataaacag attattgagc aagccgaggc gtttcttgct gcctacggca tgagcaggtt
     8341 tgcccctgtc gactatgacc ctgccagcct gccgatttct gagctgtacg gctacaggga
     8401 aagtgacggg cgctatgaca aacctgtttt gttctacgtg ctgccacagc cgtttatctc
     8461 acatgtggcg aatgggttta acaaggatgc agtagcaaaa gcccttcatg aagccggaat
     8521 gctgaaaaag ccgaccagcg gtaaggggtg gcaaatcagg acgccacggc taaaacatct
     8581 gaagggggca cgcctgagag tgtacggcct tttgcttgca caggacgatg ccgaaagtga
     8641 ttaatctcgc gtgtaggcgg taaaaacagg ttgtcccagt tgtcccggtt agtaacgtgt
     8701 aaatataaca tattgaatta tatatatttt gactgtgaaa agcgggacaa ctatgggaca
     8761 acttcagggc attttgggac gactttaagc tattttggga caacctgcgt ttacctgact
     8821 gaaccacaaa aaaacatcag gttgtcccaa ttcaccctat gagttgtccc aaaatcgacg
     8881 aaggtcgtcc cactttgcag agcgtgtttt caattaaatc aattgcttat gtgtgtatgg
     8941 gacaaccggg acaactggga caaccagttc tgtatatata tatacgaaaa atcgaggtgt
     9001 ttatgtttat tgaagccata ccaaaaagca tttctccggc aattcctggc tggtgggcaa
     9061 gattcaaaga tgacgattgc ggtgagtggt ggagtccggt cgctgcatgg gcgatttgtg
     9121 atgtgtttta ttccggtgat tctgaaactt actccgagat attaccggtt ctcactggcg
     9181 ataacggaat gacaccacat cacagcgcag agggatattg cgaatgcctg tatttacctg
     9241 acacaaaatt cattctttca gatgagccag gaacgcttgc atggcatccg gctgacaaca
     9301 cagcggaaca ggtgacaacc atcaacgtta aaaccggaga gcagacagca tgacagcaca
     9361 gatagccgct tacgggcggc tggtggacga cccgcaggta aaacagacca gcaagggcac
     9421 accgatgacg ctggcgcgta tggcggtatc actgccatgc agtcaggcac aggacggaca
     9481 ggcgacctta tggctgtcgg tcatcgcatt tggcaagtat gccgacttcc tggctaaaca
     9541 tcagaaaggc gatgttgcca gcgtatccgg cgcaatgcag gtaagccagt ggaccggaca
     9601 gaacggggaa acgcggcagg gttatcaggt tattgcagac agcgtaatta gtgccagaac
     9661 ggcgcgaccg ggtggcaaaa aaggccagca gggccaggct actgacgctc taaatagggc
     9721 taagcaacag gcggggaatg atgatccata cggtgataac ataccctttt agcgataaca
     9781 gggggataca tgccagtgac atttgaagaa gttcagcaac ataaaaaatt tcatggtttt
     9841 gatgatctgg aaaccacaac agcaaaaaaa tatcgccgtc tgctttcttc cgatgcgttg
     9901 tttgttgtgg atcatcatga ttttctgcgt agctcactga ccggggaaat tttcgcaacc
     9961 aaccgtgagc aggtggaagc gatgatcgaa tatctgtgga aaataagacg cagaatgcgg
    10021 gatccagtga aacaataaag cgataaaggc ctggattttt ttcaggtctt tttttcaggt
    10081 tttgtaaatt atttgttcgt ggttgttcca ggttgttcgg tggttctggc tgatgtttac
    10141 atactgattt ttatgtatat gttggcgtgt ggcactcaga cgtgagccgc cacaatgccg
    10201 cctgaccccc tgcgcgatgc cgggttgatc tgcgagatgc cgagagtgtc gggcggcgct
    10261 ccctccgtgt tggtttcacg ttctgaatct taaccaatac gagaaaacct tcatgaagaa
    10321 attaatcgaa ctccgccagc aaaaaaccgc cctgaaaaac cagatgcgat ccctgctgga
    10381 aaaagccgac agtgaaaacc gcagtctgac cgatgacgag ggcaaacagt ttgatgaact
    10441 gcgtgcaaaa gccgattccc tcgacacaga aatctcccgt cttgaagctg ttgcggatga
    10501 agaacgtaac cagccaggtg tttccgtcga agagaaaatc accaaagatg aactgcgctc
    10561 ttatattctg accggggaaa cccgcaacct gtccggcagt gtcccggctg atggtggtta
    10621 tacggttatc ccggaactga acagagaaat tatgcgtcag ctttctgatg aatcggtgat
    10681 gcgtaaaatc tgtaccgtta aaaccattca cagcaatgaa tttaagcaac tggtttctgc
    10741 cgggggtgcg gtcgttgaac acggcgaaga aggcactaca cgtaaccaga cggcaacacc
    10801 aaaactgaat gaagtcagta tccgcctgta tccgatctac gcttacccga aaaccactca
    10861 ggaaattatc gatttttccg aagtcgatat tatgagctgg ttatcttctg aaattggcga
    10921 cactttcgtt gataccgaag aaacggatct ggtttccggt gacggcgaga aaaaagctaa
    10981 aggtttcctg gctttcccgc gcactgcaga taatgataaa acccgtcctt tcggtacgct
    11041 ccagacgaaa aaagttactg gcagcatcag cgccgacatg ctgattgacc tgaaatttac
    11101 gctacgcaat aagtaccgca aaaaagctgt atgggtgatg aactcaaata cagccgcctc
    11161 cgcacaaaaa ctgaaaaatg ccactggtga ttacatctgg cgcgatcgtt tacaggctgg
    11221 tgatcctgat tctctgctgg ggcttccggt tgaatatctg gaatttatgc cggacggtgt
    11281 gattgcagta ggtgacttta agcgcggtta tttcatcgtt gaccatgaaa ccggtacgcg
    11341 aacccgtccg gacttcagcg aacccggatt cattaacatc tatacccaga aatatctggg
    11401 cggtggtgtg gtggattcga acgccatcaa gattctggaa attcaggctg gcaagtaatg
    11461 agcaaagggg gcttcggcct cctttttcag ctttatggag tacaccgatg aaaaataccg
    11521 attttgaaat ccgtacatct gaactgaccg ccagcgataa aaaactggtg ggttacgccg
    11581 ttcgctggaa cagcctttca gaaattatct gggacgaatt ccgcgaacaa ttcatgccgg
    11641 gggcttttgc tgactatctg gcggcgggta atgatgtgcg ctgcctgtat gagcatgact
    11701 atacccgact gctggggcgc accaaatccg gaacactggt actgactgag gacaacaccg
    11761 ggctacgttt tgaactgaca ccgcctgaaa cccagcctgg aaaagatgtg cttacgctgg
    11821 ttgagcgtgg tgacattaca ggaatgagct ttggctttcg cgcattatgc gaagagtgga
    11881 atatcgcgca aaaaccgtat ctgcgtactg ttaccgccgc agaactccgt gaaatcacga
    11941 taacgtcgat gcctgcttat cccgaatccg gcgtggagat tgcccgccgt tcgttgtttg
    12001 cacagcaccc tgaattacgc ccgacagaaa ataatcgtca tcgctgggct gaactggcgg
    12061 ggttgtgata tgtggtggcc ttttagtcgt aaaaaaagcg atctgcgtaa cctgtccatt
    12121 gatgattttc tggcgctgtc cggcgtaccg aataccggat ccggagaata tgtttctgcc
    12181 gggacggctg aatcattgcc tgcagtgatg aacgcggttt ctgtcatcgc tgaggcggtg
    12241 gccacgatgc cgtgttatct gtatctggta cgtaatgaca agggcaggga ggcgcgggaa
    12301 tggctggaca gtcacccggt agatattctg ctgaatgagc agcctaattc gtgccagaca
    12361 ccttaccagt ttaaacgcac aatgatgcgt cactgtctgc tgaacggtaa cgcctatgcg
    12421 gttattgagt gggggcggga cggtcagcca aaatcacttc atccttatgc gccggggtgt
    12481 gttgtaccgg aacgcactgg cgcgcacaaa tatcgctata ccatcaccga accctataca
    12541 ggaacggtgc gcacgtattt acaggaagaa gttctgcatc tccgctatgc ctcggatgat
    12601 ggctttctgg ggcgttcccc cgtcacgatt tgccgtgagg cgctggggct tggccttgct
    12661 caacagcgtc acggagccag cattatgaaa gatggcatga tggcggcagg gattatcacg
    12721 tcaggcgaat ggctggacgg cgtgaaaggt aaacaggcat tagacgccct ggaacgctac
    12781 aagggggcga aaaatgccgg aaaaacgcca atccttgaag ggggcatgga ttacaagcaa
    12841 ctggggatga gtaaccagga tgcggaatgg ctggcctccc gtcgcttctc cattgaagac
    12901 atcgcccgga tgttcaacgt gtcgccgatt tttttgcagg agtacagcaa cagcacctac
    12961 agcaatttca gtgaggcaag ccgcgcgttt ctgaccatga caatgcgccc gtggctggcg
    13021 aactttgaac agcaaatcaa ggccgctttg ctggtggctt ctcccgtacc tggtacccgt
    13081 tatctggttg agtttgattc agccgattta ctgcgcgcca cccctaccga acgttacgcc
    13141 acgtatgaga aagggattaa gaacgggatc atgaatccga acgaagcccg cgaacgtgag
    13201 gggatgccgc cgcgtgaagg tggcgacgaa ttcagccagg catggaagca gacggtggaa
    13261 attaaaggtg aaaaagatga gtgaagaaaa aattacacct gatgaagtca gggcacatct
    13321 tcgccttgat gacttttccg gagaaggcga actcctgaaa gtgtataccg atgcggcgct
    13381 ggaagcctgc cagaagcaca tcgggaaacg ttttgaagac gggctggaat ttaccccggc
    13441 aatacgtgtt ggttgcctga tgtacatcgc tttcctgtac gagaacaggg aagcggtttc
    13501 acctgtggag cagtcggaac tgcctatggc tatttctgcg ctctggtcag tttatcgtga
    13561 tgtgggggtg tactgatgcc gtggcaacca ttaaggcgtt gtactgaacc gggctgtaat
    13621 aagcgcgtga agtccggcaa gtgtgaagag cacaggcggg ctgcatggcg tgcagaggat
    13681 gccagacggg gacaccgccg cgcgcgcggg tattcccgac agtgggacaa ataccgcgcc
    13741 ctgtacctga gcaaaaaccc gttatgcgtg cgttgtctgg ctaagggtat ttatacgcca
    13801 gctcttgtgg tggatcacat cattcccatc aatggcggcg gtgatgttct cttctggcct
    13861 gagtggaacc accaggcatt gtgccagacg tgccacaacc gtaagacgac acgggaagat
    13921 ccagccacga aagcgaaccg taaggcgggc atgtatcgcg agcaggaaga acgggcggca
    13981 catcgtaacg actggatgta tggcgatgat gactgaacag gagcaaaaca ggctgatacg
    14041 tggactgata aggcagcgtg acttatggaa gacacaggag acagggcaca aagccaacaa
    14101 gacagggcgc acagaacgca ccacagcgaa gcgattaacc gaccgtgacc gcgaggtcat
    14161 ggaatgtttt cgcaatcgct ggtgaggccg tcagaggggg tgggggaggt tttcaggacg
    14221 aaaccgcccc tgccggacac cgaccgcctc ctcaaatttt tgtgcacgg
//
LOCUS       DAZFFG010000116        14147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFFG010000116 DAZFFG010000000
VERSION     DAZFFG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.116"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..931
                     /gene="espX5"
                     /locus_tag="ACHJ7J_003948"
     CDS             <1..931
                     /gene="espX5"
                     /locus_tag="ACHJ7J_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO5002122.1"
                     /translation="KVVYFINNVTVHNNTIEIPQTVNGGYDFSHLSLKGIVIKDEDLS
                     NSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDDSNFAQIKMTDGTLNSCS
                     AMHVQFYNATMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYINLFRADLSFSKLDL
                     INFEHADLSRVNLNKATLQNINLIDSKLFFTRLTNTFLEMVICTGSNMANVNFNNANL
                     SNCHFNCSVLTKAWMFNIRLYRVNFDEASVQGMGITILRGEENISINSDILVTLQKFF
                     EEDCTSHTDMSQTEDNIHAVAMKITADIMQDAD"
     gene            complement(1085..2734)
                     /gene="yjcE"
                     /locus_tag="ACHJ7J_003949"
     CDS             complement(1085..2734)
                     /gene="yjcE"
                     /locus_tag="ACHJ7J_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5002123.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2885..4234)
                     /gene="ghxP"
                     /locus_tag="ACHJ7J_003950"
                     /gene_synonym="yjcD"
     CDS             complement(2885..4234)
                     /gene="ghxP"
                     /locus_tag="ACHJ7J_003950"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO5002124.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4780..5244)
                     /gene="soxR"
                     /locus_tag="ACHJ7J_003951"
     CDS             complement(4780..5244)
                     /gene="soxR"
                     /locus_tag="ACHJ7J_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO5002125.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSVRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5330..5653
                     /gene="soxS"
                     /locus_tag="ACHJ7J_003952"
     CDS             5330..5653
                     /gene="soxS"
                     /locus_tag="ACHJ7J_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO5002126.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5656..7242)
                     /gene="pdeC"
                     /locus_tag="ACHJ7J_003953"
     CDS             complement(5656..7242)
                     /gene="pdeC"
                     /locus_tag="ACHJ7J_003953"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO5002127.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCSPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYFRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7671..7952
                     /gene="yjcB"
                     /locus_tag="ACHJ7J_003954"
     CDS             7671..7952
                     /gene="yjcB"
                     /locus_tag="ACHJ7J_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO5002128.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(8051..8587)
                     /gene="ssb1"
                     /locus_tag="ACHJ7J_003955"
     CDS             complement(8051..8587)
                     /gene="ssb1"
                     /locus_tag="ACHJ7J_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO5002129.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8841..11663
                     /gene="uvrA"
                     /locus_tag="ACHJ7J_003956"
     CDS             8841..11663
                     /gene="uvrA"
                     /locus_tag="ACHJ7J_003956"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO5002130.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11698..12054)
                     /gene="yjbR"
                     /locus_tag="ACHJ7J_003957"
     CDS             complement(11698..12054)
                     /gene="yjbR"
                     /locus_tag="ACHJ7J_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5002131.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12058..12474)
                     /gene="yjbQ"
                     /locus_tag="ACHJ7J_003958"
     CDS             complement(12058..12474)
                     /gene="yjbQ"
                     /locus_tag="ACHJ7J_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO5002132.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12585..13298)
                     /gene="aphA"
                     /locus_tag="ACHJ7J_003959"
     CDS             complement(12585..13298)
                     /gene="aphA"
                     /locus_tag="ACHJ7J_003959"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO5002133.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGKNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            13699..14040
                     /locus_tag="ACHJ7J_003960"
                     /pseudo
     CDS             13699..14040
                     /locus_tag="ACHJ7J_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502670.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3769 a         3587 c         3379 g         3412 t
ORIGIN      
        1 taaggtggta tattttataa ataatgtcac cgtccataat aatactatcg aaatcccaca
       61 gacagtaaat ggtggttacg atttttcaca ccttagcctg aaaggtatcg tgattaaaga
      121 tgaagattta tccaattcga attttgcagg ttgcagacta caaaacgcta tttttcagga
      181 ctgtaatatg tataaaacga attttaattt cgccataatg gaaaaaatac tttttgataa
      241 ttgtattctc gatgactcaa atttcgctca gataaaaatg actgacggaa ctctaaattc
      301 atgttccgct atgcatgttc aattctacaa tgcaacaatg aatagagcca atattaaaaa
      361 taccttcctt gattattcaa atttttatat ggcatacatg gctgaggtaa atctttataa
      421 agtaatagcg ccatatatta atttatttag agccgacctt agcttctcta aacttgattt
      481 aattaacttt gaacatgctg atctgtctcg tgtcaacctg aataaagcaa ccctccagaa
      541 tataaactta attgatagca aactcttttt tacgcggtta acaaatacgt tcctcgaaat
      601 ggttatatgt accggctcta atatggctaa tgttaatttt aataatgcca atttaagcaa
      661 ttgccatttc aactgttctg ttttaacaaa agcctggatg tttaatatcc gtctctatcg
      721 tgttaatttc gatgaggcta gcgtccaggg aatgggtatt accattctcc gtggtgagga
      781 aaatatctcc attaatagtg atatcctggt aacactacag aaattctttg aagaagattg
      841 tacctctcat actgacatgt cacaaactga ggataatatt catgcagtcg ctatgaagat
      901 tactgcagat attatgcaag atgcagattg aggttaagtc gaagaacagc caatgaattg
      961 actttaaaag agagataact tatttgtttt agaatcatga atgttagccc gagctaacct
     1021 gttaaaggtg caaaagattg cacctttttc aaattacctc gggcctggtt acgtcgcttt
     1081 cggcttactg attttcctca atcaacaacg cttcaagcaa atcgagatcg tgcagtaatt
     1141 tttgcagcgt ttcgttgctg atctcccgcg tggcgcgcag gtggtacagt tcagcacgtt
     1201 cagaacgcaa tgccgccaga cggaagcgac gctcaaggtt ctcttcctgc acggaacttt
     1261 caacgtcgtt acgtccatcg gcgcgacgac gcaggttacc aatgacgcga gaactgacct
     1321 ccgtaagcaa ctggttatcg atattctctt cggtatccgc cgccagacgc tcctccattt
     1381 tctggatcgc cacaatcgcc acttctgccg ttgccgctcg cgcaatacgt tcctctttca
     1441 attgttgcga atggtccgcg acttcaatgt gttgtagcag aataggcaac atcaccacgc
     1501 cgacaaacag cgagaagaga atgacaccag ccgccagaaa caccagctca tagcgcgccg
     1561 ggaagacgtt accatccggc aagagcagcg ggattgagag cacaccggcc agagtgatcg
     1621 ccccacgcac cccagcgaaa gacgcgatca ggatttctcg tgtggtccac gaaccaaact
     1681 ccatcggctt cttcttcagg aagcggttgc tgaacttttt catcgtccac agccagccga
     1741 aacggaccag catcagcgcc gcatatatca gaataatatc ggtaaacagc atccagattt
     1801 cgacgttagg gtcgatttct gccgccatca gcgacgtctc cagaataccc ggtagctgca
     1861 gacctaacag caggaacacc atgccgttaa agacaaattc cagcatcgcc caggtgctgt
     1921 ttgcgcgcag gcgcattgcc agtggcgcac ggcgcatcac accggagcgg gtgatggtca
     1981 tcccggcggc aaccgcagcg aggatgccgg aaacgccaat atgttcggca atcagataag
     2041 aagcgaatgg cagcaggaac agcaggacga tctgcgtcgc gggttcatca ccgccccagc
     2101 ggctgaggaa tcgcagcgag cgaccgtaca gccagctaac cacaaaaccg gcgagaatac
     2161 cgccaatggc tactttcata aattcgaccg tcgcaccgcc gacggtgaag atcatcgtcc
     2221 ccatcgccac tgctacggca aacttcaacg acaccagacc ggaggcgtca ttcatcaacg
     2281 cttcgccctg caaaatgccc atgatttttt tcgggatgcg cccttcgcca acaatcccgg
     2341 agagcgccac agcatcggtc ggagaaagta ccgccgccag cgcaaaggcg gggatcagcg
     2401 gaatgcccgg caccacccag taaataagga agccaatgcc gaccacggtg accaccacca
     2461 gcgccagtgc gaggccgaaa atctctcgac catgttcgag aaattcacgg gtcggcgttt
     2521 tccagccatc agcgaacagc aacggcggga taaacaagac taaaaagagt tcaggatcaa
     2581 actccacatg caaaccaaac gtcggccacg ccagtagcgc accgatggcg atttgcataa
     2641 gcggaagcgg aatctgaaag ggcatgacac gagtgaccac cccggacagc gagaccacga
     2701 gggtcattat cagtatggtg aagaagattt ccatgcgttc cctgtttgcg atgtttttta
     2761 tgatctgaga aagacgttat gcctgaattc tcattttttc agaatatcgc aaaagctcac
     2821 acaatgtgcg gagaaaataa aaacgggcag ccattgccac ccgttttcag aatccgatga
     2881 aagattagat agcccagcca cccgcataga aggtcaccag cgccacggcg ataacgaccg
     2941 taccgatgtt caacttgcgc cattcaccgg aaaccagacg accaatcacc agagtcgcga
     3001 agccgatcat gatgcctgtt acgatgttac aggtcagcac gatgaatacc gccgtaacca
     3061 gacccgccat cgcatcaaca aaatcagcaa agtcgatttt cgccacgttg ctcagcatca
     3121 gcaggccaac gtacatcagc gccggagccg ttgcgtaccc cggaacgagg taagagagcg
     3181 gagagaggaa cagaatcagc aggaacagca cgccaacggt gatagccgtc aacccggttt
     3241 taccgcccgc cgccgtaccc gccgcagact cgatgtatac cgctgccgga gctgcaccca
     3301 ccaggccaga gaaaacgctg ctcatggagt cagtggtcag tgctttcccg ccgtcgatga
     3361 tctgcccatc tttatccagc aggttcgcct ggccggcgac ggcacggata gttccggtgg
     3421 catcaaatac tgccgtcatc accagcgcca gaacgcttgg caggactaca ggattcagcg
     3481 cgcccataat gtccaggctg ccaatcagtg aattgccgtt ttcatcgctc aatgaaggca
     3541 tggcgaaaac gccggagaaa tggacgttag gatcgaagat caaaccgaca attgagatac
     3601 cgataatggt cagcagaatg ccaccaggga ctttcagttt ttccaggccg atgatcaccg
     3661 ccagacctac cagtgacata atcaccggga aggtcgcgaa atcacccagc gcaacgggca
     3721 gaccatcaag cgggttttta atcaccagac cgacaccgtt agcggcaatg agcagcagga
     3781 acagaccgat accgatcccc gtgccgtgcg ccacaccgtg cggcaagttg cgcaaaatcc
     3841 agctacggat acccgtggca gaaatgaccg taaacagcac acccatcagg aacacggcac
     3901 ccagcgcgac aggtacgcta atatgttgcc ccagcaccag actgaatgcg gtaaacgccg
     3961 tcagggagat ggcgcaacca atcgccaacg gcagattagc ccacagcccc atcacgatag
     4021 aaccgagtcc ggcaaccaga caggttgcaa cgaaaactgc cgcaggcggg aagcctgctt
     4081 tacccaacat acctggaacg acgataaccg agtagaccat cgccagaaac gttgttaacc
     4141 cggcaaccac ttcctgacgg acagtgcttc cacgttgtga aattttaaac caggcgtcga
     4201 gtgaaccgcc ggtacgcgct gatggcgtag acataagaaa acatcccctg agagtttaat
     4261 tttcgtcaag atgcgtggcg gacaatcccc tgccagcgaa acgttatctt cctgctccag
     4321 gtttgcgaca atttgctgcg tcgcaaaaaa gcaaacgttt agctccgcgc atacaaaacg
     4381 ggtggtaaaa aaagcaaacg attatccggc gatcatagcg gctttttcaa ccgaagtttg
     4441 ctgctttttt ctcttttatt gtctttacga agaaaaacca ggcaattgat gcgcaaacgt
     4501 tatcgtcggt gcgcaagatt gcagcgttat cgagccgtat accgttctgg attgcgataa
     4561 aaaaacgtct aagatgacaa aaatattgcg gctaaaaagt atacttttac actggcgaag
     4621 aaacgccggt gacatacaat taaagcatca acaccaaccg gaacctccac cacgtgctcg
     4681 aatgaggtgt gttgacgtcg ggggaaaccc tcctgtgtac cagcgggata gagagaaaga
     4741 caaagaccgg aaaacaaact aaagcgccct tgtggcgctt tagttttgtt catcttccag
     4801 taagcgtgcg ccggtacctt cttctcctaa gcggtcgccc gggttacgca acgggcaatc
     4861 actgcgcgaa aggcagccac aaccaataca tccatccagt tcgtcacgca gcgccactaa
     4921 ggtatgaatg cgccgatcca actcttctcg ccattgggac gaaagctgtt tccactcttt
     4981 cgcacttaac gtatgccctt cgggcaacac gccaaacgct tcaccaatgg tcgccagcgg
     5041 aatgccaata cgctgagcaa ttttgataat tgcgacatat cgcaacacat cacgtttata
     5101 tcggcgctga ttgccgctat tacggacact ggtaatcaac cctttgcttt catagaaatg
     5161 cagcgccgat accgccacac cgctgcgttt cgccacttcg ccgggggtta gcagcgcttt
     5221 aatgcggggt aatttctttt ccataaatcg ctttacctca agttaacttg aggaattata
     5281 ctccccaaca gatgaattaa cgaactgaac actgaaaaga ggcagattta tgtcccatca
     5341 gaaaattatt caggatctta tcgcatggat tgacgagcat attgaccagc cgcttaacat
     5401 tgatgtcgtc gcaaaaaaat caggctattc aaagtggtac ttgcaacgga tgttccgcac
     5461 ggtgacgcat cagacgcttg gcgattacat tcgccaacgc cgcctgttac tggccgccgt
     5521 tgagttgcgc accaccgagc gtccgatttt tgatatcgca atggacctgg gttatgtctc
     5581 gcagcagacc ttctcccgcg ttttccgtcg gcagtttgat cgcactccca gcgattatcg
     5641 ccaccgcctg tagttttatt gcccgcgcgt taactcccgc gcgggtaatt gctccatcca
     5701 ttgcataaac acctgcggcg gcatcgcctt cgcaaagaac catccctggc aatagcgcac
     5761 accgcgtttg cgcagccagt taacctgctc ctcagtttcg acgccttcag cgatcgtttt
     5821 taaccccagg ctgtgcgcca gctcgatgat atgttcagca atcaaatgac tggttttatg
     5881 ggtggtcagc gtctcaacaa acgatttgtc gattttcaaa atatcgacat tcaatgattt
     5941 aaggttatgc aagttagagt agccaatacc aaaatcatca attgccactt cgtaaccagc
     6001 ctggcggaaa gccagaataa tcggcgtcat tttgtcgaca tcaagaaatg catgctcagt
     6061 cacttcaaat ttaatttgct gcggacgcac cgcgtattgc tctgttttct gattgattcg
     6121 cgctatcaac cgtgacgtat gaaaatcgga ggccgacaga ttaatagaaa catagcgatc
     6181 agcatgtgtt gcaaggtacg cgcccagatc gcggaagaca ttatcaataa cataatcagt
     6241 tacctgttct atcatcccct ccttttctgc cagcggaata aactctgccg gattcattac
     6301 ttgcccctgc tcaccaggcc aacgtaacaa cgcttcagcg ccgatacatt tttctgtttt
     6361 gatatcgatt attggctggt aataaagaca aagttgatgt ttttctaggg cgcgttgcaa
     6421 tttgcgtttg ggagacaaat agttttgtcg aatacgtagc cagagtaata gcagaacaag
     6481 actccccaaa ataccggcgg gtaacgcaaa aataagatga ttataaaaat gggctataag
     6541 acgttgatat gaagtcgcaa caatggctgc aattgggcgt tttgtcgaat aaactgtcgc
     6601 atataaatag ccatttcttt gtacggttaa atccttcaaa tgaatcagcg gagaaaacgt
     6661 tgctgccgag gcctcgttgc ttaacgagaa aaaggttttc gtcaccgtat catacacacc
     6721 ccattgcaat gtcgggtcat cagacatcac ttcgctccag aagagagggt tgataaccgc
     6781 cacataattt ccccgctgca tataggtcat tttatagcca gagaaaaaag gcgtatcgcg
     6841 gaaataatag atagaaacgt taggttcacg cttataatcg gccggtgcaa tcgtatagcc
     6901 gtttacaggc gctatcagtg atgagcataa aaaatggtta tcacgggcat agatcaattc
     6961 attaatataa agatagccac gaataatatt taacattcgc ttttgatggg ctggagagca
     7021 aacttgcccc tgatagcgtt ctgctgcatc gctcactaaa tctgcctggc gaatgaccag
     7081 ctcggattta tccagtgcca gttgagcaaa ggttcggagc tgattattca cttctgattt
     7141 tgcccaaagg aaggcaatcc atagcgaaag aatgatggga aagagaacta aaaagataat
     7201 gcccggcaac gccagtaatt ggtgtcgtgc acgatgactc attgctatca atttcctgcc
     7261 tgtttatacc cggccattgc aaggtgaaac cctggcgacc gacgtcttta gataaatctt
     7321 acggtataaa tagcactttt gcctgatttg aaattgatac ctttttaatc gtcacctgga
     7381 tgaaaataat tcattgatat tcaaaattaa ttttctacag gaatacagag gcggcgggaa
     7441 gatataccct taatgccact gaaaaaccac ataatctgta cagcaataaa agtcacgacc
     7501 taatctggct aacaaacaat caattgtgat atagttcaca aaattaatga aacaaacaga
     7561 gtgtttcatt tttgtgttct gcaagccccg cgatagccag agtgtaccac gcctcccgtg
     7621 aacaacgccg cgctgtccag ggcatcggct cttttcagag gaattgtttg atggctaccc
     7681 tcaccactgg cgtggttctt cttcgctggc aacttcttag tgccgtaatg atgtttctgg
     7741 ccagcacact caacatccgt tttcgtcggt ctgattatgt cgggcttgca gtgatcagca
     7801 gcggtctggg cgtggtttcc gcctgctggt tcgcaatggg gttgcttggc atcacaatgg
     7861 cggacatcac cgccatctgg cacaacatcg agtcggtgat gatagaagag atgaatcaga
     7921 caccgccaca gtggccaatg attttgacct gatacgtaga agcctccata acggaggctt
     7981 cttttttatg gctggcagat gctttaatca tccaccttaa aacaatataa cctattgttt
     8041 taatgacaaa tcagaacgga atatcgtcat caaagtccat cggcggctcg ttagacggcg
     8101 ctgccggagc ggactgctgc gggcgagact gcgcgccgcc gctgaactga ttgccaccct
     8161 gcggctgctg aggctgaccc caaccgccct gcggctgacc accaccgata ttgccacctg
     8221 caggagcgcc accaccctga cgaccaccca gcatctgcat agtgccgcca acgttcacca
     8281 caacttctgt ggtgtagcga tcctgaccag attgatcggt ccatttacgg gtacgcagct
     8341 gaccttcgat ataaacctga gaacctttac gcagatattc gctggccact tctgccagtt
     8401 tgccgaacag cacaacgcgg tgccattcag tctgctcttt catctcgccg gtcgctttat
     8461 cacgccagga ttcggaagta gccagcgtaa tgttggcaac tgcgccacca tttggcatgt
     8521 agcgtacttc cgggtcctga cccagattac caacgagaat aaccttgttt acgcctctgc
     8581 tggccatgtt cgtgtctcct gaaaaaaatc gttctgaata agtgtaaacg cgcgattgta
     8641 ccattaccaa tagcgctttt actatgttgt gacctcggtt ccgggaaaca aacctggcca
     8701 gacattgtta cacaacactc cgggtaatgc attccaatac tgtatattca ttcaggtcaa
     8761 tttgtgtcat aattaaccgt ttgtgatcgc cggtagcacc atgccaccgg gcaaaaaagc
     8821 gtttaatccg ggaaaggtga atggataaga tcgaagttcg gggcgcccgc acccataatc
     8881 tcaaaaacat caacctcgtt atcccccgcg acaagctcat tgtcgtgacc gggctttcgg
     8941 gttctggcaa atcctcgctc gctttcgaca ccttatatgc cgaagggcag cgccgttacg
     9001 ttgaatccct ttccgcctac gcgcggcagt ttctgtcact gatggaaaag ccggacgtcg
     9061 atcatattga ggggctttct cctgccatct caattgagca gaaatcgacg tctcataacc
     9121 cgcgttctac ggtggggaca atcaccgaaa tccacgacta tttgcgtttg ttgttcgccc
     9181 gcgttggcga gccgcgctgc ccggaccacg acgtcccgct ggcggcgcaa accgtcagcc
     9241 agatggtgga taacgtgctg tcgcagccgg aaggcaagcg tctgatgcta ctcgcgccaa
     9301 tcattaaaga gcgcaaaggc gaacacacca aaacgctgga gaacctggca agccagggtt
     9361 acatccgtgc tcgtattgat ggcgaagtct gcgatctttc cgatccgccg aaactggaac
     9421 tgcaaaagaa acataccatt gaagtggtgg ttgatcgctt caaggtgcgt gacgatctta
     9481 cccaacgtct tgccgagtcg tttgaaaccg cgctggagct ttccggtggt accgcggtag
     9541 ttgcggatat ggacgacccg aaagcggaag agctgctgtt ttccgccaac ttcgcctgcc
     9601 caatttgcgg ctacagtatg cgtgaactgg agccgcgact gttttcgttt aacaacccgg
     9661 caggtgcctg cccgacctgt gacggcctcg gcgtacagca atatttcgat cctgaccgcg
     9721 tgatccagaa tccggaactg tcgctggcag gtggtgcgat ccgtggctgg gatcgccgca
     9781 acttctatta ttttcagatg ctgaaatcgc tggcagatca ctataagttc gacgtcgaag
     9841 cgccgtgggg cagcctgagc gcgaacgtgc ataaagtggt gttgtacggt tctggcaaag
     9901 aaaacattga attcaaatac atgaacgatc gtggcgatac ctccattcgt cgtcatccgt
     9961 tcgaaggcgt gctgcacaat atggagcgcc gttataaaga gacggagtcc agcgcggtac
    10021 gcgaagaatt agccaagttt atcagcaatc gcccgtgcgc cagctgcgaa ggaacgcgtc
    10081 tgcgtcggga agcgcgccac gtgtatgtcg agaatacgcc gctgcccgct atctccgaca
    10141 tgagcatcgg tcatgcgatg gaattcttca acaatctcaa actcgcaggt cagcgggcga
    10201 agattgcgga aaaaattctt aaagagatcg gcgatcgttt gaaattcctc gttaacgtcg
    10261 gcctgaatta cctgacgctt tcccgctcgg cagaaacgct ttccggcggt gaagcccagc
    10321 gtatccgtct ggcgagccag attggtgcgg gcctggttgg cgttatgtac gtgctggacg
    10381 agccgtctat cggcctgcac cagcgcgata acgagcgcct gttgggtacg cttatccatc
    10441 tgcgcgatct cggtaatacc gtgattgtgg tggagcacga cgaagacgcg attcgcgcgg
    10501 ctgaccatgt gatcgatatc ggtcctggtg cgggtgttca cggcggtgaa gtggtcgcgg
    10561 aaggtccact ggaagcgatt atggcggtgc cggagtcgtt gaccggacag tacatgagcg
    10621 gtaaacgcaa gattgaagtg ccgaagaaac gcgttccggc gaatccggaa aaagtgctga
    10681 agctgactgg cgcacgcggc aacaacctga aagacgtgac gctgacgctg ccagtcggtc
    10741 tgtttacctg catcacaggg gtttcaggtt ccggtaaatc gacgctgatt aacgacacac
    10801 tgttcccgat tgcccaacgc cagttgaatg gggcgaccat cgccgaaccg gcaccgtatc
    10861 gcgatattca ggggctggag catttcgata aagtgatcga tatcgaccaa agcccaattg
    10921 gtcgtactcc gcgttctaac ccggcgacct ataccggcgt gtttacacct gtgcgcgaac
    10981 tgtttgcggg cgtaccggaa tcccgtgcgc gcggctatac gccgggacgt ttcagcttta
    11041 acgtccgtgg cggacgctgc gaagcctgtc agggcgacgg cgtgatcaaa gtggagatgc
    11101 acttcctgcc ggacatttac gtaccgtgcg accagtgtaa aggtaaacgc tataaccgtg
    11161 aaacgctgga aattaagtac aaaggcaaaa ccatccacga agtgctggat atgaccatcg
    11221 aagaggcgcg tgagttcttt gatgcggtgc cagctctggc gcgtaagctg caaacgttga
    11281 tggacgttgg cctgacgtac attcgcctgg ggcagtccgc aaccacactt tctggtggtg
    11341 aagcccagcg cgtgaagctg gcgcgtgagc tgtcaaaacg cggcaccggg caaacgctgt
    11401 atattctcga cgagccgacc accggtctgc acttcgccga tattcagcaa ctgctcgacg
    11461 tactgcataa actgcgcgat cagggcaaca ccattgtggt gattgagcac aatctcgacg
    11521 tgatcaaaac cgctgactgg attgtcgacc tgggaccaga aggcggcagt ggcggcggcg
    11581 aaatcctcgt ctccggtacg ccagaaaccg tcgcggagtg cgaagcatca cacacggcac
    11641 gcttccttaa gccgatgctg taatcgttaa ggccgctttc cgagcggcct tttcctttca
    11701 gagttgcacc agcaatttac gtttttcttc cggcagtaaa ttcaccgcct gctgataaga
    11761 cgcatccacc agataataga tttgcgaatc tggcagcgaa ccgtcgagat acacggtgct
    11821 ccagtgcgct ttattcagat ggcggcttgg acgcacatcg ctgtgctgct gacgtagcag
    11881 ctccgccagc tccgggctgg ttttcagcga aacagctggg cgattttcta cttctttcac
    11941 catcgcaaac agtacatctt ccactttgat ctgcgtcgct ttccagtcgt tatgcacgct
    12001 ctgttctgcg cctggttttg ccatgcaata ttgtagcaac tccgaaatgg tcatttttta
    12061 ctccccttgt agtgtcgcga tgatgcgacg cgatccgccg tggatgcgat gttcccccag
    12121 ccaaatgcct tgccaggtgc cggtctgaat acgcccttta tgcaccggca atacaagcga
    12181 tgttcccagc attgaggatt tgatatgaga aggcatatcg tctgctccct catagtcatg
    12241 ctcataattt ccgttgtcgg gaacggtgcg gaggaaaaaa cgctccatgt cgtggcgtac
    12301 ggtaggatcg cagttctcat taagtgtcag agaggcggag gtatgttgca gcaacagatg
    12361 cagtaagccg atgttaacgc gcggcatatc agccagctga ttcagaattt catccgttac
    12421 cagatgaaac ccacgagatt tggcgctaag cgtgagcgtc ttttgatacc acatgtgctg
    12481 ctccttgata aaactctctt aatcagtttg cagcaagaca gcgaaaggat aaaggtgtga
    12541 ttaaaaaaac agcattcggg agagtgtcac gctctcccgc tctgtcagta ttctgaattg
    12601 acgatcacct cttcaccaaa cgcacccgct tgtggcaagg gtttgtaggt agagttggag
    12661 gcgcgcagaa tgcggatacc acgagcgccg acatcgcgtg cagcggtaat atcgttatcg
    12721 gaatcgccat aaaaaattcg gatattttta tcctgcagcc attgcgattt tgtatttttc
    12781 cctggtttat cgcccgcaaa gatcaccgga ttcatgttgg tggcaggaat atgaaaatta
    12841 tccgccagcg tttttgaaac cgtttctgtt ttcgtcgggc tacgaccagt cacaaagaag
    12901 atcgcgtcac cgcggcgtac atgcatatca atcagctggc gagcgacctc ttttggaatg
    12961 ctgaattcat cccagccgtt gttcattttt tcccagaaca caggattttt cagataatct
    13021 tcgctttctg gcgagaaggt ttttttgccg cgccagaagc ccgggctgga aaaaagtacc
    13081 gtgtcatcga tatcaaaccc caccgccatt ggcggacgcc ctgcgaggct attttcaatt
    13141 tgtgcgaccg aaacccaatg aattggtgcc tgttcagcaa gcctggcaac gttagtccca
    13201 gggttaagcg gtgaaggaga tgaggccagg gcaacagcgg aactgtttag agcgaacaat
    13261 aagcaaacgg cactgattgc ctgtgtgatc ttgcgcatat ttttccctaa atagtcagta
    13321 tgttgaaact ttttattgtg agatttgttg caaaaactat ctgaccataa cgccagctac
    13381 aggggaaagg aaggattatc tgcggttttt gtgatgaaaa gcaggatgat tatatttaac
    13441 ccagtaaata cgttcctgtc gtttttttgt aaaaaatggt tattgctcga tgtaaggata
    13501 cattaatttt acgtttatta tgatgaatac tctgcaaatg agcgaaattt gatagaacat
    13561 ttatctgctg tacgatttaa tgaacttaat gatacagcat tacaaaaaca atcgaagttt
    13621 ataaagatga tttctgattg accagaaccc taccctacaa tggtgattaa ttttattact
    13681 cccgaaggag atgattatat gaatatatca tatgtgaata gcaacaaaac gacatcttta
    13741 cctattgagt tagatgcaca aaataacaaa gatttttctt atgccaaaga tttcttttta
    13801 tatatagaaa cacagttaaa aatcgcaaaa gatttttgta gacctgaaga agtatcaagt
    13861 tctattgcaa gtacagtttt tcacgctttt attgatttag ttaacaaaat caggggtaag
    13921 aaagattgta tgtatatttg cacgctttgc tgttttgccg aggaggttaa aggtgattat
    13981 tctcattaca ggaccttttc atttgatatt ggtaatcaat ataaggttaa acttacacaa
    14041 agcggaaaaa atgagttctc tttaacttta gaatttaatg atactgaaat tgaatgtcag
    14101 aaagtcacag gcaataaggc aaagcatatt cttgaggata tagcaga
//
LOCUS       DAZFFG010000117        14049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFFG010000117 DAZFFG010000000
VERSION     DAZFFG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.117"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(118..1656)
                     /gene="cadC"
                     /locus_tag="ACHJ7J_003961"
     CDS             complement(118..1656)
                     /gene="cadC"
                     /locus_tag="ACHJ7J_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO5002134.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1916..2075)
                     /locus_tag="ACHJ7J_003962"
                     /pseudo
     CDS             complement(1916..2075)
                     /locus_tag="ACHJ7J_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2273..2348)
                     /locus_tag="ACHJ7J_003963"
     tRNA            complement(2273..2348)
                     /locus_tag="ACHJ7J_003963"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2313..2315),aa:Phe,seq:gaa)
     gene            complement(2455..3030)
                     /gene="yjdC"
                     /locus_tag="ACHJ7J_003964"
     CDS             complement(2455..3030)
                     /gene="yjdC"
                     /locus_tag="ACHJ7J_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5002135.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDIWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(3067..4764)
                     /gene="dsbD"
                     /locus_tag="ACHJ7J_003965"
     CDS             complement(3067..4764)
                     /gene="dsbD"
                     /locus_tag="ACHJ7J_003965"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO5002136.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKYLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(4740..5078)
                     /gene="cutA"
                     /locus_tag="ACHJ7J_003966"
     CDS             complement(4740..5078)
                     /gene="cutA"
                     /locus_tag="ACHJ7J_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO5002137.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(5194..6495)
                     /gene="dcuA"
                     /locus_tag="ACHJ7J_003967"
     CDS             complement(5194..6495)
                     /gene="dcuA"
                     /locus_tag="ACHJ7J_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO5002138.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(6613..8049)
                     /gene="aspA"
                     /locus_tag="ACHJ7J_003968"
     CDS             complement(6613..8049)
                     /gene="aspA"
                     /locus_tag="ACHJ7J_003968"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO5002139.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            8386..8862
                     /gene="fxsA"
                     /locus_tag="ACHJ7J_003969"
     CDS             8386..8862
                     /gene="fxsA"
                     /locus_tag="ACHJ7J_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO5002140.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(8878..10134)
                     /gene="yjeH"
                     /locus_tag="ACHJ7J_003970"
     CDS             complement(8878..10134)
                     /gene="yjeH"
                     /locus_tag="ACHJ7J_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO5002141.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPVRDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            10410..10703
                     /gene="groS"
                     /locus_tag="ACHJ7J_003971"
     CDS             10410..10703
                     /gene="groS"
                     /locus_tag="ACHJ7J_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO5002142.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            10747..12393
                     /gene="groL"
                     /locus_tag="ACHJ7J_003972"
     CDS             10747..12393
                     /gene="groL"
                     /locus_tag="ACHJ7J_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO5002143.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            12531..12884
                     /gene="yjeI"
                     /locus_tag="ACHJ7J_003973"
     CDS             12531..12884
                     /gene="yjeI"
                     /locus_tag="ACHJ7J_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO5002144.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(13077..13946)
                     /gene="yjeJ"
                     /locus_tag="ACHJ7J_003974"
     CDS             complement(13077..13946)
                     /gene="yjeJ"
                     /locus_tag="ACHJ7J_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO5002145.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         3674 a         3364 c         3521 g         3490 t
ORIGIN      
        1 taaatttatg taataaaaat tatgcggcaa ataaattgcc gcaacatatt ataccaacag
       61 gaacatacaa aaactcaaca acaaatattc ccgagcatga atcaacccgg gggttactta
      121 ttctgaagcg agaaatttgt cgagataagg tacaacataa ggaacagaag tctggaatat
      181 accattttca atccagtaaa gggtgtttgc ccctgggcgt aaattaaagg cggtgagata
      241 tgcatcagcc gcttcccggt tcatcccctt catttcataa accttgccaa gcaatacata
      301 atttagccag gacatttcaa gatcaatgcc agtatttatc gcctggtaag actcatctgt
      361 tttacctttt accagagcac tgaccgcttt tatttgatat ataatggaca ggttattcaa
      421 ttccggtagt gtaacaatgt tatctatttc tgtgttcagt gctgctaatt gtttttcatc
      481 taaaggatgt tgagaatggc gcacgatatc aactaatgct ttttctgctc tcgcgtaggt
      541 aaattctggg gatgattgaa caatctcacc taataattca ctggcacggt tcaatgattt
      601 atcatcgcca tgcagtaaat aatcatgtgc ctgataaaaa ttagttaata acgcaccacg
      661 atgaggcaaa atttgctgga gcgtctcctg cattcgttgt ggccacggtt ggtttaacgc
      721 ttttgataaa ctctccagta aatcattttg aatcgccagc tgattaccgt tagtgatgac
      781 ataacgttta tccagcatgg ttgaaccatc tgcattgtct actaatttta tcgacataaa
      841 gcattgttga gcacggtatt ggcgctgatt aacaaacgca atagataatg ttttaccgga
      901 actgctcggt tcatcaatgt tgtagttgat tttgtcatgc accataaagg tggagaaggt
      961 gttaagtgat gtcgccacca aatcacccac gcctatcgcg taagagagct gatacgggga
     1021 actccagctg ttacaacttt tatttaccat attaatgtca atatcgcgtg gattgagcaa
     1081 aatacgcgat ttgctcatcg gaagacgtgt atcaagactt gaaaacgcta ccagtgctac
     1141 acagatacct aaggacaaca ggaaaaaaaa ccatacccaa aaggtagtga atcgtttgct
     1201 tttaactggg gattgttcag gtggcgttgc ggtgttttga atgttaagac tgtgggaggg
     1261 agaatctgtg gcaggaaccg cctctggtat agggggaggc gaagatagca ttatttcctc
     1321 tccctcttct tcgctgtacc agataaccgg caccattaat ttatagccgc gctttggtac
     1381 agtagcgata tagacaggac tatcttcatc attatctttt aatgacttac gtagttctga
     1441 gatactctgt gtcacaacgt gattggtgac aatacttctc ttccagacat tatcgataag
     1501 ttcatccctg ctaagtactt cgccactgtg ttgagcaaag aaaaccagaa ggtcgattaa
     1561 tctcggctca agggtaagtt gacgcccatt gcggctaatt tggtttatgg acggagtaac
     1621 aagccattcg ccaacgcgaa ctacaggttg ttgcataata gaaactcatt cgaaaaggga
     1681 atgatgcaat gataattgcc acaacctatt tttaccatct atagatgggt ttatttacat
     1741 attattggtg aatgcaagac gttattttta ccagccataa acttcttgat tacatggtat
     1801 tacgaaagga ttttactgag aaccagaagt aatattcctt accatcaaaa ttcatcatct
     1861 ttgccaaaga aaaatgttca gaaaataatc catggaaaat tgtccggagc gcttactatt
     1921 ttaatggatt gttagtcttt gcatgagcaa gcggactgat acatttctct ttgttctcat
     1981 tcagaaaatc tcatcagtcg ccgttcagac cggtgttgtg ctttatggat gcgttacgct
     2041 cctgatgacg tcatttgacg ttcaacagca tcacggggcc gcacgacatt tcacgtcagt
     2101 cagtgctata gctcaggaac taattttccc gaattgggat atgcccgcaa attactggtg
     2161 atgtggggag aatctggttg agatcggtag aattgatttg gaggcagaac gcttaaatcg
     2221 tggcgtcctg aaacgaaaaa cggacctccg tggaggtccg tttatatgaa tttggtgccc
     2281 ggactcggaa tcgaaccaag gacacgggga ttttcaatcc cctgctctac cgactgagct
     2341 atccgggcaa cggggcgcat taaaccgtaa tctgcacatc tcgtcaacct aatttcagta
     2401 aaagcgattc aactgcttaa gattgcggca aatcgcttct tttctgtgtt tctgtcaggt
     2461 caatgcgcca ccctggcggc agcgggcgaa gcgcaggata tcttccgcca gccgatgtgc
     2521 agtgtcgaca tccgcctggc tacgatttac cagcattcgg cttaaacagc cttccagcac
     2581 cagttccatc tgctttgcta ccatcgccgg atcgtcaact tccaacgtgg ttaacagttc
     2641 gtgggtgaaa tcgtaggccg cgcttttttg ctgatcggcc agttgatgaa tagggtggcc
     2701 aggatcggga taaaacgtac aggcagcgat aaatagacag cccggatagc ggttgttttt
     2761 aacgcactcc gataacgcct gataacgtgc cagcagcttt tgttcggcgg tttgcgtttc
     2821 gtccagcatc agctgacgac gccagatatc tatctgttgg ctaagataac gcagcgcatc
     2881 gtagaggatt gcctctttgt ctggccagaa gcggcgtagc tcgtccagtg gataatccac
     2941 acgttcagca accatctcca gcgtggtgtt ggcaatccct tgtaattcta ataatttcag
     3001 ggcttctccc agtacatctt cacgttgcac gctattttcc tccgtctttc ccactgcaag
     3061 tgtcgttcac ggttggcgat cgcgcaaatg tgcgctgaag gtttcagcat ccataaagcc
     3121 cgtgacgcgt gcttgtggat gctcctggcc ttgtccgtca aaaaagagaa ttgtcggtag
     3181 accaaggaca ttaagatact ttaacagcgc cacatcttgt gcgtcgttgg ccgtgacgtt
     3241 ggcctgaagt aagaccgtgt ctgctaacgc tttttgcacc tgcgggtcgc tgaaggtgta
     3301 tttctcaaac tctttacagg cgacgcacca gtcggcataa agatctaaca tcaccggttt
     3361 gcctttggct tcaacgagcg cctgatttaa ctcatctacc gttttgattt gtgtaaagtt
     3421 gagatgcgtc tgagtttgcg cggtatgcgt cgcaccaaat gcccaatcct gaagtgggcg
     3481 cacgctaacc aatgccgctg ccagcaggat tatttgcacc acgcgcatcc agccgcgttt
     3541 ggcctgtagg ctggtgataa aggcccagcc aaagaatgcg acacccagcg ccgaccacaa
     3601 gcgtaatccc catatatcac caatcactcg ctccagcagg aagaccggca gtgcgaggat
     3661 cacaaaacca aacgcggttt tgacttgttc catccacggg ccgcttttcg gcagcaagcg
     3721 gttaccaaag acggtaatta gcatcagcgg caggcccatg cccaacgcat agagataaag
     3781 cgtgccgccg cccagccaca tgttcccgct ttgggcgata tacagcagaa tcgcgctaag
     3841 cggtgcggtg gtgcatggtg aacagatcag tccggcaatc gcccccataa taaacactcc
     3901 gccgggtgag ccgccctgtt ggcgattgct catcagcgtg aggcgcgttt gcagcgagga
     3961 ggggagttgt agagtaaaca agccaaacat tgacatcgcc agcaaggtaa agacgatggc
     4021 gaggccaatg agcacgtatg ggtgctgtag cgccgcctgg aactgtaacc ctgcggcggc
     4081 aactaccaga cccagcgccg tgtaagtcag tgccatcccc tgcacataaa taaaggtcag
     4141 caacaatgct ctggcagtgg agagccgctg tttaccaccc agcacgatgc cagaaatcag
     4201 tgggtacatt ggcagcacgc atggcgtaaa ggcgatacca ataccgatca acaacgccca
     4261 gagcgcggaa aagggcaatt gcgcggtggg ctgctcttgc tgcggaacag acacaggctg
     4321 tgacgctgcg ttgttggcga ccacttcgct taacggaacg gttttggttt ctggtggata
     4381 acagaaaccg gcatcagcac agccctggta ggtgacagtt aacgtcgctc ccgcactcgc
     4441 ctggttgatg gttacgggaa gtgtcagccg atcgcggtaa atctcgcttt tgccgtaaaa
     4501 ctcatcttca tgccagacgc cttgcggcag ctgcacgtcg gcaattttcg cgtgttccgg
     4561 cgtaatgcgg atctgtttac ggtagaggta gtaaccgtct ttgatctgcc aggtcagatt
     4621 aaggtcatgt tggttttgct gaaaatcaaa agcaaaggct tgatccgcgg ggacaaattg
     4681 tgaacgtccc ggcgcgtcga ataatccggc aaaaacggaa gtgctgcaaa gtagcaggat
     4741 cagcgtaaag atgcgttgag ccatgagagg taatctgtgt ctccgtgtgt aacaggtaaa
     4801 accagaagtt ccggggtttg atatggatga tgagacttca ggcattccag cagtgcctgc
     4861 tggtgagata cggtagtttt taaaatcatc tgcacttcgt attcttgctc cagcttacct
     4921 tcccagtaat agagagaggt agcgccgggg atcaaggtcg cgcaggccgc cagtttttcc
     4981 gccagcactt tggcggctaa atcctgggct gtcgcttcat ccggtgcggt acatagcacc
     5041 acgacagacg cggtattcga acttttttca tcaagcataa acacctcgca agaacagatg
     5101 gaaccgcaag agagaggtca ctatacaacg gacgggggaa ggatgttagt cggcgggaaa
     5161 gaaagcgggg cgtgaacgcc ccgcgattgg tcattacagc atgaagctac ccagcacgaa
     5221 gccgaagcaa acggccaggg caacacccag agtacccggg atgaagaacg gatggttgaa
     5281 gacgaattta ccgatacggg tagtacccgt gtcatccatc tgtaccgcag caaccagcgt
     5341 cgggtaggtc ggcagaatga acagaccaga caccgcagcg aaagaagcaa cagcggtcag
     5401 cggagaaacg ttcagtgcca gagccatcgg catcagtgct tttgcggttg cagcctgaga
     5461 gtacagcaga gcagaagcaa agaagaagat gacggccagc agccacggat gaccctgaat
     5521 cacttcacca gcggtatctt tgatccagtc gatgttgttg gaaacgaaag tatcgcccag
     5581 ccacgcaaca cccaggatac aaatacaggc gctcatacct gctttgaagg tgctggagtt
     5641 gaggatgttg tcggtatcga ctttacagat aacggtggtc agagttgcaa cgctgagcat
     5701 gatgatcagg attgcgttgg tggtgttcat cagcggtttt tcaaccagac ccatgcttgg
     5761 gctgttgatg attgcataga taaccacgcc aactacgccc agcaggaaca gccagacgga
     5821 cgtttttgca ccggatttga tttcaatctg cttttcaccg cgcagttcaa ccaggccctc
     5881 ttccagacgc ttgcgataaa tcggatcgtc agagagtttg gagttgaaca gcatagtgac
     5941 caggaaggac atcaccagaa ccgccagcag ggtggacggg atgaccacgg agagcagatg
     6001 gaggtagctg atgccatgac cttccatcac ggaagacatg taaaccactg ccgctgagat
     6061 tggcgatgcg gtgatcgcaa tctgcgcgga taccactgca gtagacagcg gacggcaagg
     6121 tttaacgcct tgttccttcg caacttcagc gataactggc agtgtcgcca gagagatgtt
     6181 gccagtacca gcaaagatag tcaggaaata ggtcacgatc ggtgcgagga tcgtgatgta
     6241 tttcgggtta cggcgcagca gcttttctgt ctgatgaacc agatagtcca gaccgccagc
     6301 aacctgcatg gcagaaatag cggcgataac cgccatgata atggaaatga catcgaacgg
     6361 gatgttaccg ggtttaacgc caatagcggc aagaaccagc acccccaatc cgcctgcaaa
     6421 accaatacct attcccccca atctggcgcc caagaagatc gccagcaaaa ctatgatgag
     6481 ttctacaact agcatattag ccttccttgt tttttaacaa gttgatatta gattgttatt
     6541 tttaagttac tgctcacaag aaaaaaggca cgtcatctga cgtgcctttt ttatttgtac
     6601 taccctgtac gattactgtt cgctttcatc agtatagcgt tttgctttgt aagccgggtg
     6661 catcagattc tgtacggaga aaatatcgtc aagttccgct tcagtcaaca gaccgcgttc
     6721 cagaacgact tcacgtacac tcttaccggt ttcggcacag attttaccca cgatgtcacc
     6781 gttgtggtga ccgatgaacg ggttcaggta agtaacgata ccgatagagt tgtaaacgta
     6841 accttcgcac acttctttgt tagcagtgat gccgttaatg catttttcca gcaggttgta
     6901 gcaagcgttg gtcagaatgt gaacggattc gaacatagcc tggccaatga ccggctccat
     6961 aacgttcaac tgcagctgac ctgcttctgc tgccatggta acagtggtgt cgttaccgat
     7021 gactttgaag catacctggt taaccacttc cggaacaacc gggtttactt tagctggcat
     7081 gatggaagag cccgcctgca gttccggcag gttgatctcg ttcaggccgg cacgtggacc
     7141 agaagagagc aagcgcaggt cgttacagat tttggacatc ttcacagcca ggcgtttcag
     7201 cgcgccgtga accataacat aagcgccgca gtcagaggtc gcttcgatca ggtcttcagc
     7261 cggtacgcat gggaagccag taacttcagc cagttttttc actgccagcg gagagtactc
     7321 tttcggcgtg ttcagaccag tgccgattgc tgttgcgcca aggttaactt ccagcagcag
     7381 ttcagcggta cgttggatgt ttttcacttc ttctttcagc agtatgctga aagcgcggaa
     7441 ttcctgaccg agggtcatcg gtactgcgtc ctgcagctgg gtacgaccca ttttcaggat
     7501 gtcctggaat tcgacagctt tacgttcaaa gccttcacgc agttggttaa tcgcatctac
     7561 cagcttaatc agggaagagt aaactgcgat acggaaaccg gtcgggtagg cgtcgttagt
     7621 ggactgacat ttgttaacat ggtcgttcgg gttcaggtac tgatattcac ctttctggtg
     7681 acccatcagt tccagaccga tattggccag cacttcgttg gtgttcatgt ttacggaagt
     7741 acctgcgccg ccctggtaga cgtctaccgg gaactgatcc atgcattttc cgttgttcag
     7801 gacttcatca catgcggcaa tgatggcatt cgctacactt ttaggaatgg tttgcagctc
     7861 tttgtttgcc atagctgcgg cttttttaac cattaccata ccgcgaacaa attcaggaat
     7921 atcactgatt ttgttgttgc tgatatagaa gttttcaatc gctctcagag tgtgaacacc
     7981 atagtaggca tcagctggaa cttccctggt acccaacaga tcttcttcga tacgaatgtt
     8041 gtttgacatg tgaaccttct ttttcaagct gccaatgatt tgctttaaac acacagaata
     8101 tatgtggttt cgaatgtttt tcgaccgacg attatcccct gcatcgaccg aatacccgag
     8161 atcatatgct gcttgaggat ttctaccgta atctggatca ctttaagtgt cggtttttac
     8221 cccttaatta ttaatttgtg aaatagatca ccgctttggg attactacca aaaatagttg
     8281 cgcaaacatc ttgaaatttt gctaatgacc acaatataag ctaaacgcga ttcgcaaccc
     8341 attcaggtag ccgggtttta ccggctgcta ttacaggaga aacctttgcg ctggttacct
     8401 tttattgcca ttttccttta tgtctatatt gagatttcaa tctttattca ggttgcccat
     8461 gtattggggg tattgctgac cctcgtgctg gttatattca cgtcagttat cggtatgtca
     8521 ctggtacgta accagggctt taagaatttc gtgctgatgc agcaaaaaat ggcggcgggt
     8581 gaaaacccag cggcggagat gattaaaagt gtttcgctga tcattgccgg tttgctgctt
     8641 ttattacctg gctttttcac cgacttcctc ggtcttctgc ttttattgcc gccggtgcaa
     8701 aagcatctga cagtgaagtt gatgccacat ttgcgctttt ctcgcatgcc aggcggcggt
     8761 tttagcgccg ggaccggtgg cggtaatact tttgatggtg agtaccagcg aaaggatgat
     8821 gaccgcgacc gccttgatca taaagacgat cgccaggatt aatgtcgaaa cgccggatta
     8881 tgtggttatg ccattttccg gcgtttttcg ttttggcaga aacagccata accccgccag
     8941 catgatcagc gcatagagac ttttccagcc gaccattgtc agtaacagaa cgcataacag
     9001 cccgccaacc accgccagta gtcgataacg tccttgcaat aatttacagc ctgccagcat
     9061 gcataacaga taaatcataa taaagatgcc attggcataa ataataagag cgtccagatt
     9121 gatctctaaa gcatgaatca ccaaagtgct caccacgcag cagccgagca ccgcattgag
     9181 ggcattattc gggatatggc gagaagagag gcgtgccagg tagtggtcag gattatgttg
     9241 cgcctgcgac cagaccaggc gggcgaagct ctgtatataa atgttgagac tggcaaagca
     9301 ggccagatag ccaatcacgc aggcaatcca taacgctcct acaccgaaca actgcactac
     9361 aatttttgga agcgatgctg ccgccgccat ttgttcacca taggcgtcga agtgtaagac
     9421 gactaccgta cagccccagt agactaatcc tgccagcagc agaccaatca tcaaagcacg
     9481 aggaaaatca cgtactggat ttttaaattc cgaggcaaga tgggcaaatg cttcaagacc
     9541 gacaaaacac cagaacatca ctgataatgc agcaaataac ccggtaagtt cgatattacc
     9601 cggtgccggg aaggggatat tcgcaggttt gatatcgccc gcccaccaga tagcgacaat
     9661 cagcgcgacg ataagtccgg caataactgt ttgtagattt gcactggaac tggcaccgcg
     9721 agtaccgata taccacacca acgccagcgt accgagttct gccaacaaca gttgccagct
     9781 atgccagcca aacattgcct ggccgaatcc ggcagcaatt tgtagcgcgg caggcaaacc
     9841 cacgggaatg accgataaaa acagccagcc ggtgactcgc tcaagccgcg aaccaaacgc
     9901 cataccgacg aagtgcgcga cgccgcctgc actgggatag tggcgaccca gaatcgcaaa
     9961 cacaatcgca atcgggaaca ctaagataat caaaacgggc cacgcccaca ggctgttatt
    10021 gcccgctacc agcgcagcta acgcaggaac ggcaaacacg ccagtgccta ataatgacgt
    10081 cgatagcagg ccaatgccct gggccagccc cagttcttgt ttgagtccac tcatgggttg
    10141 atgtccgatt gcgcccaaat tttgggcaac tgcgtagatt ttcgatggta gcacaatcag
    10201 attcgcttat gacggtgatg aagaaattgc gatgaaatgt gaggtgaatc agggttttca
    10261 cccgattttg tgctgatcag aatttttttt ctttttcccc cttgaagggg cgaagcctca
    10321 tccccatttc tctggtcacc agccgggaaa ccacgtaagc tccggcgtca cccataacag
    10381 atacggactt tctcaaagga gagttatcaa tgaatattcg tccattgcat gatcgcgtga
    10441 tcgtcaagcg taaagaagtt gaaactaaat ctgctggcgg catcgttctg accggctctg
    10501 cagcggctaa atccacccgc ggcgaagtgc tggctgtcgg caatggccgt atccttgaaa
    10561 atggcgaagt gaagccgctg gacgtgaaag ttggcgacat cgttattttc aacgatggct
    10621 acggtgtgaa atccgagaag atcgacaatg aagaagtgtt gatcatgtcc gaaagcgaca
    10681 ttctggcaat tgttgaagcg taatcctcgc acgacactga acatacgaat ttaaggaata
    10741 aagataatgg cagctaaaga cgtaaaattc ggtaacgacg ctcgtgtgaa aatgctgcgc
    10801 ggcgtaaacg tactggcaga tgcagtgaaa gttaccctcg gtccgaaagg ccgtaacgta
    10861 gttctggata aatctttcgg tgcaccgacc atcaccaaag atggtgtttc cgttgctcgt
    10921 gaaatcgaac tggaagacaa gttcgaaaat atgggtgcgc agatggtgaa agaagttgcc
    10981 tctaaagcga acgacgctgc aggcgacggt accaccactg caaccgtact ggctcaggct
    11041 atcatcactg aaggtctgaa agctgttgct gcgggcatga acccgatgga cctgaaacgt
    11101 ggtatcgaca aagctgttac cgctgcagtt gaagaactga aagcgctgtc cgtaccgtgc
    11161 tctgactcta aagcgattgc tcaggttggt actatctccg ctaactccga cgaaaccgta
    11221 ggtaaactga tcgctgaagc gatggacaaa gtcggtaaag aaggcgttat caccgttgaa
    11281 gacggtaccg gtctgcagga cgaactggac gtggttgaag gtatgcagtt cgaccgtggc
    11341 tacctgtctc cttacttcat caacaagccg gaaactggcg cagtagaact ggaaagcccg
    11401 ttcatcctgc tggctgacaa gaaaatctcc aacatccgcg aaatgctgcc ggttctggaa
    11461 gccgttgcca aagcaggcaa accgctgctg atcatcgctg aagatgtaga aggcgaagcg
    11521 ctggcaactc tggttgttaa caccatgcgt ggcatcgtga aagttgctgc agttaaagct
    11581 ccgggcttcg gcgatcgtcg taaagctatg ctgcaggata tcgcaaccct gactggcggt
    11641 accgtaatct ctgaagagat cggtatggag ctggaaaaag caaccctgga agacctgggt
    11701 caggctaaac gcgttgtgat caacaaagac accaccacca tcatcgatgg cgtgggcgaa
    11761 gaagctgcaa tccagggccg tgttgctcag atccgtcagc agattgaaga agcaacttct
    11821 gactacgacc gtgaaaaact gcaggagcgc gtagcgaaac tggcaggcgg cgttgcagtt
    11881 atcaaagtag gtgctgctac cgaagttgaa atgaaagaga aaaaagcacg cgttgaagac
    11941 gccctgcacg cgacccgtgc tgcggtagaa gaaggcgtgg ttgctggtgg tggtgttgcg
    12001 ctgatccgcg tagcgtctaa actggctgac ctgcgtggtc agaacgaaga ccagaacgtg
    12061 ggtatcaaag ttgcactgcg tgcaatggaa gctccgctgc gtcagatcgt cctgaactgc
    12121 ggcgaagaac cgtctgttgt tgctaacacc gttaaaggcg gcgacggcaa ctacggttac
    12181 aacgcagcaa ccgaagaata cggcaacatg atcgacatgg gtatcctgga cccaaccaaa
    12241 gtaacccgtt ctgctctgca gtacgcggct tctgtggctg gcctgatgat caccaccgag
    12301 tgcatggtta ccgacctgcc gaaaaatgat gcagctgact taggcgctgc tggcggtatg
    12361 ggcggcatgg gtggcatggg cggcatgatg taattgccct gcacctcgca gaaataaaca
    12421 aacccccggg cagaaatgtc tgggggtttt tcttttggtc atctttctag tataagattc
    12481 agacacggac gacgcgagtg gcgtccagct cattgattat ggggaataac atgcacgtaa
    12541 aatacttagc agggattgtc ggtgccgcgc tactgatggc gggttgtagc tccagcaacg
    12601 aattgagtgc tgccggtcag agtgtacgca ttgtggacga gcagccaggc gcagagtgcc
    12661 agctgattgg tactgcgaca ggtaagcaaa gcaactggct ttccgggcaa cacggagaag
    12721 aaggcggttc tatgcgcggc gcagcaaacg atctgcgcaa ccaggcggct gcaatgggcg
    12781 gtaacgtgat ttatggcatc agtagcccgt cgcagggaat gttgtccagt tttgtcccga
    12841 cggatagcca gattatcggc caggtatata agtgcccgaa ctgatgtgat ggtgacaaac
    12901 gtataattgc cagatgcgct tcgcttatca ggcctacata aggcattgca actgattgaa
    12961 tgcttgagaa tttgtaggcc ggataaggcg tttacgccgc atccggcatg accaacgatc
    13021 acgttatcag tcatttcagg ctggatggct ccagcctcgc attagcaaag atgagattat
    13081 ttcgcctgtg gtgcagtttt gctggtggat tgaacacgta agtgatgcag cgcgcgtaag
    13141 cattgatcct gggttagcgg ttgggcgtta ttcgccgcta cggtgcgaac atagacctga
    13201 catggcacac ccacgagttt tttcagtcgt gggctgaaat caagcattcg gcgagtgatc
    13261 gcctctattt ctttacccgg cgttgcttca cgcgttttca ccactgcgcc gctaaattgt
    13321 tctttgccac tttcatcttt aaagcggtaa agcaccgcca gataatggtc gaacaagtta
    13381 tgtttccatt ccggttggga ataggtgtca tattccagat tgccattttg ctggcagtca
    13441 acgtcatata acggcaggaa atctaacagt gaggtaatac gtaatgccag ttcacgcatt
    13501 tccgcattat taattgcatg aatgattgct cgcgccagca tttcaatctg caactcattg
    13561 acgactaact cacatttgct accgtcatgt agcgttaata cgaaggtaag attttcgcct
    13621 tgattatcgg taagttccag cgtgttaacc cgacgattaa tatcggcatt tttaagctca
    13681 tcaaccagaa tttctgggat gttttctgcc attttcttaa tgactttatc gcgagcttgc
    13741 tcatactgaa ggcgtgcagc agcatccagt ttatgctttt gatgcagacg actttccagc
    13801 gcaatcagca ggtcgcgcag ttccatgaca gacataaaga acaacgattc tttattgcga
    13861 ggttctttaa ttttcagcgc cagtgcaatg aaattattac tcttgcgaat aacaccggta
    13921 ttaacaccct tgatgcttat ggccatgtag gttctcccta accatttctc aattaaataa
    13981 ttaattttaa tttataagcc agataaatgg gcttggtagt aatagttgtt aaaataacat
    14041 aaatagccg
//
LOCUS       DAZFFG010000118        14010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFFG010000118 DAZFFG010000000
VERSION     DAZFFG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.118"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..885)
                     /gene="yhaJ"
                     /locus_tag="ACHJ7J_003975"
     CDS             complement(<1..885)
                     /gene="yhaJ"
                     /locus_tag="ACHJ7J_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5002146.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLF"
     gene            990..1691
                     /locus_tag="ACHJ7J_003976"
     CDS             990..1691
                     /locus_tag="ACHJ7J_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO5002147.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHAITHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1714..1878
                     /gene="yhaL"
                     /locus_tag="ACHJ7J_003977"
     CDS             1714..1878
                     /gene="yhaL"
                     /locus_tag="ACHJ7J_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO5002148.1"
                     /translation="MSKKSAKKRQPVKPVVVKEPARTANNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2011..3321)
                     /gene="cyuA"
                     /locus_tag="ACHJ7J_003978"
     CDS             complement(2011..3321)
                     /gene="cyuA"
                     /locus_tag="ACHJ7J_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO5002149.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVVEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3349..4680)
                     /locus_tag="ACHJ7J_003979"
     CDS             complement(3349..4680)
                     /locus_tag="ACHJ7J_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5002150.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4955..6319)
                     /gene="tdcG"
                     /locus_tag="ACHJ7J_003980"
     CDS             complement(4955..6319)
                     /gene="tdcG"
                     /locus_tag="ACHJ7J_003980"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5002151.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEQFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6391..6780)
                     /locus_tag="ACHJ7J_003981"
     CDS             complement(6391..6780)
                     /locus_tag="ACHJ7J_003981"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO5002152.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6794..9088)
                     /gene="tdcE"
                     /locus_tag="ACHJ7J_003982"
     CDS             complement(6794..9088)
                     /gene="tdcE"
                     /locus_tag="ACHJ7J_003982"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO5002153.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9122..10330)
                     /gene="tdcD"
                     /locus_tag="ACHJ7J_003983"
     CDS             complement(9122..10330)
                     /gene="tdcD"
                     /locus_tag="ACHJ7J_003983"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO5002154.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10356..11687)
                     /gene="tdcC"
                     /locus_tag="ACHJ7J_003984"
     CDS             complement(10356..11687)
                     /gene="tdcC"
                     /locus_tag="ACHJ7J_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO5002155.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11709..12698)
                     /gene="tdcB"
                     /locus_tag="ACHJ7J_003985"
     CDS             complement(11709..12698)
                     /gene="tdcB"
                     /locus_tag="ACHJ7J_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO5002156.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12797..13735)
                     /gene="tdcA"
                     /locus_tag="ACHJ7J_003986"
     CDS             complement(12797..13735)
                     /gene="tdcA"
                     /locus_tag="ACHJ7J_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO5002157.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3652 a         3781 c         3280 g         3297 t
ORIGIN      
        1 aaaaagtttg ggaatttccc gcagacacca ggatttcgct tcccccatac tgtcacgacg
       61 ccaggccata ataatatcga tctcgctggt cgattccggg ctgacgacac gcaaccgccc
      121 ttccgcaata tctttttcga ccatcggata cggcatcgtc gccacgccaa gccccgccag
      181 taatgcctga cgtttatctt caatcgtgct caccgttaag cgcggctgtt tgtccagcag
      241 ctgtacggtc aacaccgggc gctcacgagc ggtatccgcc accgcaattc cacgatattt
      301 cacacgcgtc acttcagata acggttccgg ctcctgatga atcgggtgat caggcgcggc
      361 aacgtagacg ttcattaacg tatagagctt gcgcgagttg atctccgacg aggaacgaaa
      421 atgcatatcc ggcgcgataa caatatccgc ccgcccctgc tccagccgtt cccacgcccc
      481 cgccagcact tctgtgatga ttgccagttg ggtattggct tttgccgcca gtttgtcgat
      541 taacgggaaa aaggcaggtg tcggtaccag cgcttcggtc acaatggtga gatgcgtttc
      601 ccaaccgcgc gcgagagctt ccgcatcagt agtcagttta tctgcggctt ccagcaaaac
      661 gcgccctcgc tccaacaaca tcctcccgac attggtgaat ttggtgcgat ggcccgagcg
      721 gtcaaacagc accacatcca gctcttcttc cagtttttgc atggtgtagc taagtgcgga
      781 aggcacgcgt cccagctcat ccgctgccgc cgcaaaactg ccccggcgat cgatcgcatc
      841 cataacccgt agtgcttcca gcgttaatgc cctttctttg gccatttcgt tctcattcag
      901 gaaatttgaa cataccgggc agaatatctg gctaacaatg tagcgtccag ccccttacca
      961 taaaaggaag taaagagagg tcaagaatta tgattactac ccgaactgcc aggcagtgtg
     1021 gacaagcaga ctacggatgg ttgcaggccc ggtatacttt ttcctttgga cactacttcg
     1081 acccgaaatt gttaggctat gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag
     1141 gtgccgcctt tcagccgcga acctatccca aagtcgatat tttaaatgtg attctggatg
     1201 gagaagcaga gtatcgcgac agcgaaggca atcatgttca ggccagcgcc ggtgaggcgt
     1261 tgctgctctc tacccagccg ggtgtcagct acagcgaaca caatctcagc aaagacaaac
     1321 cgttaacacg aatgcagctt tggctggacg cctgcccgca gcgagagaat ccgctgattc
     1381 aaaaactggc gcttaatatg ggcaagcagc aattaatcgc ctcgccagag ggtgcgatgg
     1441 gaagcctgca attacgccag caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa
     1501 gtgcgaattt ccagttgcat ggaccacgcg cgtatttgca atcgattcac gggaaatttc
     1561 atgcgattac gcatcatgaa gagaaagcgg cgctgacctg cggtgatggg gcgtttattc
     1621 gtgacgaggc taacattacg ctggttgccg attccccact gcgcgctttg ctgatagatt
     1681 tgcctgtcta gttgttttta cagggagatg atgatgagta aaaaatcggc caaaaagcgc
     1741 cagccagtga agcccgtggt ggtgaaagaa cctgctcgca ccgccaataa ttttggctat
     1801 gaagagatgt tgagcgaact agaagccatc gtcgcggatg ctgaaacgcg tttagccgag
     1861 gatgaagcta ccgcgtaaag acaggtgccg gatgcgacgt aaacgcctta tccggcctac
     1921 aaaacacaac aacatcaaca aattgcgcac cccgtaggcc ggacaagatg cgccagcatc
     1981 gcatccggca cgctccccaa aacctggcgt ttatctggcc ttgctcgcca taatctcgat
     2041 aatctgccga tccgtttgct gcatcgaatg gcttgctaat gcacacaggt tggcaatcga
     2101 ctgctcaaca tcatgcgcca caatcccttc attgccggtc acggcggtat catccagcgc
     2161 cattaacacc gctttccacg cagccgaagc actggtcgaa accttcatcg cgcagctgtt
     2221 cgacgcacca tcgcaaatca tgccgctgac atcgccgatc atactgctga tcgccatcga
     2281 gatggtttca taacgcccat ccaccagcca tgccatcccg gcggcggccc ccattgctgc
     2341 ggtcgttgcg gcacacagcg cagacaaacg cggtaactgg ttatggatgt aaattgcgct
     2401 caaatgagaa agcatcagcg cacgcgccag ccgttcatca tccgctccga agtgttctgc
     2461 taccaccacc acaggcattg ttgcggtgat cccctggtta cccgagccgg agttactcat
     2521 agccggaagc gtagcgccgc ccatacgcgc atcggatgcc gcgctggtac gaatcacaat
     2581 ggatgaagag agatctttcg ccagcaaacc gcgcgcgcac tgtttttcca gcgtcgcgcc
     2641 aatatgcagc ccccacttac cgctcaaacc ttcctgcgat aacgcgcaat ttagcttcgc
     2701 ggaatcgaga ataaagcgga tcgccgcaaa cgggacttca ttgacgaact tcaggatctc
     2761 agccagcgtc gttctggaaa gcaccgtcag cggagactct tgctcgccct ctaccacaca
     2821 cgcctgctgg gtaaacacca caccatcgtg cgtctcaata tgcacaatgt tggtatgccc
     2881 gccgacgatg gtgacacacg cccacttctc accgttccag actttggcgc gtgagaagag
     2941 gatttcattg caaggttcct ggatcttaac ggagactttc cccgccgcca gcagtgcttt
     3001 ggcatcggca attgcctgcg cagttgcgtc tttcagcact tccagcccgg cgttggcatt
     3061 tccacctaac gcccccagcg ccgccgcaat cggcagcccc accattcccg tgccgggaac
     3121 ggtgacgccc agaccgttct tcatcagatt tggcgaaacc caggcttcta cacgttcaac
     3181 cggaccttcc agttctgccg cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt
     3241 acatcccagc gccggtttta cttcctcctg aacggcgagg atgtaacgct gccataacgg
     3301 atttaaagtc gaatcaaaca tattaaaaac cttaaaattt caggtaaatc aagaaaatgc
     3361 caggaacgga gaaacacaaa gcaacaaacc agtgacgata atcaggtaca gagacatccc
     3421 tttgtatttg tgcaatgccg gtactttgta aaccagccac gccgggatca ggcaccctac
     3481 catgccgaaa atcgggctac agatagaggt gaagctcaac accggtgcgt tcagtacgat
     3541 ggcgctccag gccagcaaaa tggcgaaaat catgatgccg cgctgaacaa gattttcgtt
     3601 aatcttctcg gcaggcatct tgcgacgcag gatgttcatt acgatccctt gcgttgcttc
     3661 gcgaaagcct aaatagacgc caaagaatgc agtcatgaca gcaaagatat tgagaatgac
     3721 gctgaccact ttcacccacg ctgcgccgtc accgctaata aactgtgcgg caatcgccag
     3781 cgcggaaata ttctgctcgt aggcttttac cgcttcgtca tgtcccatcg ccagcgtgaa
     3841 cgacacggcg tagaaaaaga cggtgacaaa caaaatgcca aacgcgatat tcattgcccg
     3901 caatgcttta tgccgcgcca cttcaattga tttttcccgc gagcgataag agatcaccat
     3961 cgggcttaac gtctggataa acagaatcga cgtcagggta aacggcagcg taataatggc
     4021 gtttttcacc agcagcccca gcggcggtag cgaaccgacg ttatacagat gccacattcc
     4081 caccatcgac acgcccagcg ccgcgaccac cagcagcttg gtcagcacca tgccggtcga
     4141 aattttgaat aacaatttct cgccgcgtga ggagatcgcc accagaatgc aaatcagtac
     4201 cagaccataa aagggactgt ctgacagcaa cccttccgtc acgccgaagg tatgcaggta
     4261 ggaagcacta tcgttggtga tggcggtgga atagacgaac atccaaatca ccagcattac
     4321 gaaatagagc gcacctaaca ggatgcccca gtttttacct aaataaccgc taatgacact
     4381 cgggtaatct ttacattctg gtgattctgc cagcgtatta ataaacaacc gctgaaacag
     4441 atacattgcc gggtaaccaa tcaccgatga gagcaaaaat acccacaatc ccatcaaacc
     4501 gacctgcacc gggagaaaaa caatccccgc gccaatcgcc atcccgatac tcataatcac
     4561 ccagccggtg tcagtactgt cgaatttgat cgcttctcgc cactcgctct cactcattcc
     4621 agcacgaccc gccggggtcg aagcgtctgc aatgacgcct ttattcgatg caatttccat
     4681 aatttctcgc tcaatatttt gtagggctta ttattttttt ccgagccgca tcaaggcgat
     4741 atgcggtcta cgtttttgca ggcgagttat tagaataaag aaatgatacg cgcgaggtca
     4801 gagaaaatct tcacaatcaa gactttgaaa taacggagat ggataagaat tttctactaa
     4861 attaatcgca agagaatatt cattctctat ttgcggcaga tcacaaaaaa aaggtgcaca
     4921 tttgtgcacc caaggatgaa agctgacagc aatgtcagcc gcagaccact ttaatggcca
     4981 gtcctccgcg tgatgtttcg cggtatttat cgttcatatc tttgccggtt tcatacatcg
     5041 tctcgatcac tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc gccatccgcg
     5101 cggcgtttac tgctttcacg gcattaatgg cattacgttc aatgcacggg atttgtacct
     5161 gtccggcaac cggatcgcag gtcagtccaa ggttatgctc catcgcgatt tccgccgcat
     5221 tgcatacctg cgccggactg ccgcccagta gttcagttaa ccctgccgcc gccattgaac
     5281 aggccacgcc aatctccccc tgacagccga cctccgcgcc agagatggag gcgttcattt
     5341 tatacagcgc gccaatagcc ccggctgcaa gaaaatagcg ggcaattgac cgctcgttta
     5401 ccggacgacg gaacttatcg taataagcca gcactgccgg aataatgccg cacgcaccgt
     5461 tagtcggtgc cgttaccacg cgcccgccag ctgcgttttc ttcactaacc gccagcgcgt
     5521 acatgttgat ccagtcgatg acattcatcg gatcgttaga gatgttatcg ctggaaacca
     5581 gctgacgacg cagcgctacg gcacggcgcg gcacattgag cggaccaggc agcacgcctt
     5641 cagtgttcat cccgcgttca ataccgtcat gcatcacttg ccagatacgg gcaaaaccgg
     5701 cgtcaatttc cgctttactg cgcagcgcta gctcgttgtg catcatcagg ccagaaatag
     5761 acaggccgtt gtaatcacac attttcagca gttcacctgc tgagtggaaa tcgtaaggta
     5821 cggacgtttc gacatcgtgc gacaggccga actgttcttc ttcgacaata aacccgccgc
     5881 cgacagagta ataggtttta cttaatagct cttcctgtgc cttccaggca gtgatccgca
     5941 ttccgttctc atggcgaggc aacatttcgg gatggaagat aatgttcttt gctacaggaa
     6001 aatcaacaat atgcgcacca gatgccactg gcagccgccc gctgcgcgtt actaactcta
     6061 taaatgcagg gatctcatca atgacaacat cctgcggact gtttcctgcc agtcccatga
     6121 tgatggcgac atccgtggca tggcctttgc ccgtcagtga caacgacccg tacagatcga
     6181 ccacaatatg gctcgtcgcg gttaataagc cgctactttc cagccgatca ataaaacttt
     6241 ttccggcatt cattggcccc acggtatgcg aactggaggg accaatccca attttgaaaa
     6301 tatcgaatgc actaatcata tccacaccct cggattgccg ttcagtgaag tggagcggaa
     6361 cgaccttacg accgtcccgc tcacgaggct ttacgcacta cgtactgcga tagcttcaat
     6421 ttccagcttc acatctttcg gcaaacgcgc gacctgtaca cagctccggg tcgggtaggt
     6481 cgcctgatgc tcatcgaaga actgcttata gacttcgttg atggtggcaa aatcattcag
     6541 atcggtgatg aacacggtca tcttgatgat atcgcccacg ctcagcccgg cagcaaccac
     6601 gatcgctttg acgttttcga ggcttaaacg cgcctgatct tgcacatcag ccgggatctc
     6661 accggtctgt gggcaaaccg gaatttgacc ggaggtgaag accatgctgc ctaaatcaac
     6721 gccttgaaca taagggccga ttgcgcctgg ggcacgttgc gtttcgataa tctttttcat
     6781 acatcctccg gcgtcagagc gcctgggtaa aggtacgtga aataacatcc tgctgctgtt
     6841 cacgggtcag tgcgttgaag cgcacggcgt agccagagac acggattgtc aggttaggat
     6901 atttttccgg gtgctcgatg gcatccagca gcatttcccg attcattacg ttgacgttga
     6961 ggtgttgacc gccttcgaca tccgcttcgt ggtggaaata cccatccagc aggccgacaa
     7021 ggttggtttt acgtactgga tcttctttgc ccagcgccgc agggacgatt gagaaggtgt
     7081 acgagatccc gtctttagcg taggtgaacg gcagtttcgc caccgacgtc aatgaggcca
     7141 cggcaccttt gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac ggtgttccgg
     7201 cgcgacgacc gtccggcgta ttaccggttt tctggccgta caccacgtta gaagtgatag
     7261 tcagaatcga ctgggtaggg acggcgttgc gataggttgg cagcgcttta attttcttca
     7321 taaaacgttc aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg ttgccgtact
     7381 gcggatattc accgtcgatt tcaaagtcca ccgccaggcc gttttcgtca cggattggtt
     7441 tcacgcgggc atatttgatc gcagacaggg agtccgttgc caccgacagg cccgcgatgc
     7501 cgcacgccat ggtgcgatag acatcacgat cgtgcagcgc catcagcgaa gcttcgtagc
     7561 tgtacttgtc gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc accgccagcc
     7621 agtccatgaa gtgatcgagg ctgtccatca ctttgtcgta atccagcaca tcgtccatca
     7681 acggtgctgt tttcggcccg acctgaatct tcagcttctc gtccaccccg ccgttaattg
     7741 cgtacagcag cgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt tgcttaccaa
     7801 tcaccattgg gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag tcagtacgca
     7861 tcagatcgtc gttttcatac tgcaaggaag aggtgacgat cgacacctgc gcggcatatt
     7921 ttttgaaggc aatcggtaat tcttccgacc aaagaatggt caggttaggt tccggtgccg
     7981 gccccatagt gtgcagggtg tgcaaatagc ggaaggagtt tttggtcacc agcgtacgac
     8041 cgtccagccc catcccgccg atcacttccg tcgcccagat tgggtcgccg gagaacagcg
     8101 aatcaaattc cggcgtacgc aagaagcgca ccatacggat cttcatgatg aagtgatcga
     8161 tcagttcctg tgcctgctgc tcattgagga cgccagcttt aaagtcgcgc tcaatgtaga
     8221 tatccaggaa cgatgccgtg cgacccagcg acatcgcgcc gccgttctgt gatttcactg
     8281 ccgccagata agcgaagtag agccactgca ccgcttcctg cgcattctgc gccgggcgag
     8341 agatatcaaa gccatatttc gccgccattt cctgaatctg caacagcgca tgacgatgct
     8401 ctgccagctc ctcacgcaga cggatggtgg cttccagatc ctcgcctttt tccagacgag
     8461 actggagatc ggcaaattgc agttcgcgtt cacgcaccag ataacgaatg ccatacagcg
     8521 ctacgcggcg atagtcaccg ataatgcgcc cacgtccata accatctggt aaaccggtca
     8581 gtacgccaga tttacggcag cgcagcatat ccggtgagta aacatcaaat acgccctggt
     8641 tatgggtttt acgcagatcg gtaaacagat attcaaattc actgtccatt tcacggccat
     8701 aggcgtggaa tgaactttta atcatattaa tgccaccgaa cgggtgtagc gcacgtttca
     8761 acggcgcatc cgtttgcagg ccaacaattt tttccagcgg ctggttaata tatcccgcat
     8821 catgagcggt aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg tgggttgcgt
     8881 tttcgatacg gatgccttcc atcacctttt cccacaattc cgtggtggca ggcgtcgctt
     8941 cggcgagaaa agattcatcg ccttcatacg gtgtatagtt atgttgaata aaatcgcgga
     9001 cattaatttc gcttttccag tccgtacctt taaagccaag ccatgcgtcg gcgtacagct
     9061 tatcgctggt atcaatatct accttcatga aaaataatct ctctacaata cttcaactaa
     9121 attatgcaaa ttctgcgggc gcgttaactt tgcctaaatg aatggcatcc aaagcaatca
     9181 ttttttcttc gttagtcgga ataacggcgc aaatgacacg cgcattttca ctggaaacaa
     9241 ttcgctcgcc aaaagagtta gatcgattat tcatttctgt atcaatctct acgcctaata
     9301 cagccaaatg ttccatgacc agacgacgaa ttaaacttga attctctcct attccgccgg
     9361 tgaatataat tccatccagg cgacgtaatg aagctgcgtg tccggcaata tgacgggcaa
     9421 ttcggtgaac aaaggtttta attgccagct gcgcgcgttc gtgaccttca tgccaggctt
     9481 tttccagaac acgtaaatcc gaagaaagac cggaaatacc taataatccc gactctttat
     9541 tcactacgcg ttccaggtca cccaggctct ggttggtttg gctggcgacc caggacatcg
     9601 caccaaagtc gacatcgcca ctgcgggtac ccatcatcaa gccttccagc ggcgtcattc
     9661 ccattgaggt atcaacactc tgaccgttgc gaaccgcgca gattgacgcg ccattgccaa
     9721 gatgcgccac aaccaggccg gagtcatctt ccgccagatt cagcaaagaa tgtgcgcgct
     9781 gggaaacata gcggtgcgac gtgccgtgga aaccataacg gcgtacacct aactcttcat
     9841 aatatttcca cggcaggccg tataaataag cttccggagc catcgtctgg tggaaactgg
     9901 tatcaaatac cgccacctga gttacgcccg gaaataattg ctgcgccgat tcaataccac
     9961 ttaaattggc gtaattatgc aggggtgcca gtggagaaac gcgacggata ttatcaatga
    10021 cttcatcggt aataatggcg gactcggtaa aaatactgcc gccgtgagcg atgcggtggc
    10081 caattaaggc cacgctgtca tttaaattcc gtttttccag ttcaaatgca attgccttca
    10141 atgcaccttc gtagctgtgg tgagccagcg gtgctggctc tcccccattt acggataaga
    10201 atgcattttc cgagttaata ccgtcggcaa tacctgacat taatacttca cagtcgctgg
    10261 catcgagtac ggaaaactta atcgaagacg aaccacagtt aataaccaaa acaaccggaa
    10321 attcattcat ctcttttctc atcctgagtt acggattaaa acagtttgta tacgatgttc
    10381 aggatggtca gcagaccaat cacggtaaca aacacgttat ccagacgacc acggtatttc
    10441 gccagagacg gcgctttacg gatggcatac atcggcaaca ggcacagcag ggatgcgata
    10501 atcggtgcgc ccatggcttc aatcaggtcg aggatgttcg ggttggcgta ggcaacaacc
    10561 caggtggagc ccatgatgaa gatcatgctg atagtgttca gtttacccag cgacacttta
    10621 gttttgtcgc ctttataacc aaacttcagg accaggccat tcagaccttc cagcgttccc
    10681 agatagtgac cgaagaaaga tttgaagata gccacgagtg cgatgatgga agccgcatat
    10741 tccagtgtaa tcgcgaacgt tgttttggta ccggtcatgg acgcaaagtg gttagccaga
    10801 taagaaagca ctggaatatt ctgcgctttg gcttccgcca tgttggccgg agacagcgta
    10861 aacaggcagc taaaggcaaa gaacatcacc actgcaacca tcagcatgct ggcacgagaa
    10921 atgatttggg aacatttacg ttcggtgaag tcgcgaccga agtctttctc atactcttca
    10981 cgtttagaaa ccacgaagga agagacgatt ggcgagaagt taaaggagaa aaccatgatg
    11041 gaaatcccca gccagacagt gatcaggata ccgtcatgac cggttaacga cagcgaaccg
    11101 aggtcaacct ggtcgataac tgcagagttc cagtaaggga tcagcgacaa agaaatcagc
    11161 accaggctgg cgataaacgg ccataccagg tagctcatca ctttaaccat cagatcctta
    11221 ccaaaccaga tgacgaaagc catcagcagc aacaggaaca gcgccacaaa gccgcgattc
    11281 agcggtgcaa agccgagctg gttttcccag aacgtcataa aggtattggt aatagtaacg
    11341 ccataaatcc acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg
    11401 ccagttttac caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg attagagcca
    11461 gaaagacaca gacgcgccag cgcccggtgg caataaaacg cgatggggta tgccaatacc
    11521 aacatcagaa gaatcgggat cagtccgcca aaacctgcgc ggatagggaa gaacagcacc
    11581 ccggcgccga ttgccgtacc aaacaagcct aacgtccatg tggtatctga tttacgccag
    11641 gacgattgtt ttgtctggct ggatacaatg ctatctgaag tactcatatc ctatcctcaa
    11701 cgaattaatt aagcgtcaac gaaaccggtg atttgagaga cgcgagaaag atcgatattg
    11761 ccgccggaaa taatactgac ggtttttctg ttttgaatat attggtctaa tttaccgctt
    11821 aataatgcag cacatgccag agcgcctgcg ccttcggtga cgactttatt gcgctgaatt
    11881 aaggcaatca tactgtttct gatttcgtct tcgctgacca gcacgatgtc atcgactaat
    11941 tcacgaacga tttcgtaagt taaattaccc gggcgggaga catcacaacc atccgccagg
    12001 gtgccggtag ttcggtgcgt ggttatttct ccggagtgga aagaagccgc catgccgtga
    12061 acgttttcag actgtacgcc aataacacga atggtcgggt taatagattt aattgccacc
    12121 gcaataccag caattaaacc gccaccacca attggcacaa tcacgttatc gacatcatag
    12181 agatcttcca taatttccag accaatcgtt ccctggccag caatcacttt cggatcatcg
    12241 taaggtggga taaaaatacg gccttccatt tcgacaattt cgctcacttt agcgatagtg
    12301 tcgttgaagt tatcaccatg cagaacgact tctgcggagt agtcgcacgt tgccgctact
    12361 ttggatttcg gcgcgccttt tggcatcacc actttaccgt cgatacccag catcgcgcag
    12421 gagagcgaaa ccccttgcgc atggttgccc gcagaacagg ccaccacgcc tttgcgtttt
    12481 tccgcatcag tcagtgaact taatttatta aatgcgccac gaattttaaa tgaacccgta
    12541 cgctgcatat tttcaaactt caggaatatt tcacctttgc aacgttcact aaaatagttg
    12601 gagcgaggca tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc ttcaattatg
    12661 tcatcaatag caaccggcag atcgtatgta atatgcatta taaaacctct tcgcctgaat
    12721 taaatatgta ggtaaccgta accgacacct gcaagacaga caggtggatt atttatgttt
    12781 aaataaaaac aaataactaa ccaacttcta ttaattgcct tcgtctacac ccattataag
    12841 atgaatactc tttggctaat tccaccaaaa ccgatgctgc ttttttaata cgataatttt
    12901 tcgaccatac cgcggcatat tgtgccacag gtaatgtttc ttcaaccgga atagtaataa
    12961 attgattaga accaaaaggt gacgtcatat cacaaggaat tacagttaag aaatcagcat
    13021 tgagaacaag attataaatt gtcacgactg agtcggtttt aacgatgttt tcaatactga
    13081 tgccatttct ttgtaacgta gtaagcagtt cgctgtagta ccccatatta gtttgtggca
    13141 acacccactg ttcgttcttc aacgactcca gcgtggtggt gccggtgcat gttcgggact
    13201 tactggctac cagcacaaac tcggactcga acagcggctc aacatgtaaa tcctgaagct
    13261 tcatttctgc gcttaacgta ccaatcgcaa aatccagtcg accgtcgcgg attgccggta
    13321 agaacgaaga cagttgcgct tcatacatag aaacctgcgc ttttgggaac acctctttga
    13381 acttattgat catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga
    13441 cttccaccac cgcctcagaa gacataccgc ttatctcatt aaccatattt ttcatttcac
    13501 gggtaatgga ttcggaacga gagagtaaca attgaccggc aggtgttaat gttactccgg
    13561 tatttttccg caccactaat tccacaccaa aataatcttc aatatcgtta atgattttac
    13621 tgacggccgg ttgagttaac cctaattctt ttgcagccga gccgatagaa ccacttctaa
    13681 tgacttcctg aaagactacc aggtgctgcg ttttcggaag aagaatagtg ctcataacga
    13741 cctacgttaa ttacctcatt gacggcatga agtgtatcaa aatgaaatga acaggatatg
    13801 tgcgaccact cacaaattaa ctttcaatac tttccatggt atcgtcatgt caattaaata
    13861 cctttaaata tcaataagtt aaagtataaa aatcagcata aaccctgatt tttgtcaaaa
    13921 aaaatgacgg ggataaccat attttatggc gataacatca ttcgttatgt ggttaataca
    13981 aaaaaggggc tgagaccata tttcaagcaa
//
LOCUS       DAZFFG010000119        13975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFFG010000119 DAZFFG010000000
VERSION     DAZFFG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.119"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..259
                     /locus_tag="ACHJ7J_003987"
     CDS             <1..259
                     /locus_tag="ACHJ7J_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002158.1"
                     /translation="LTKNDYLKKVPLTAQLIAKYKMDDQLDQLLVTREIQDEIKSKIQ
                     DKIDELSKNLFNTMTETIENNFDDIFRQQSENMSNYYEFVD"
     gene            complement(437..952)
                     /gene="pgpA"
                     /locus_tag="ACHJ7J_003988"
     CDS             complement(437..952)
                     /gene="pgpA"
                     /locus_tag="ACHJ7J_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO5002159.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(930..1907)
                     /gene="thiL"
                     /locus_tag="ACHJ7J_003989"
     CDS             complement(930..1907)
                     /gene="thiL"
                     /locus_tag="ACHJ7J_003989"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO5002160.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1985..2404)
                     /gene="nusB"
                     /locus_tag="ACHJ7J_003990"
     CDS             complement(1985..2404)
                     /gene="nusB"
                     /locus_tag="ACHJ7J_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO5002161.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2424..2894)
                     /gene="ribE"
                     /locus_tag="ACHJ7J_003991"
     CDS             complement(2424..2894)
                     /gene="ribE"
                     /locus_tag="ACHJ7J_003991"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO5002162.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2983..4086)
                     /gene="ribD"
                     /locus_tag="ACHJ7J_003992"
     CDS             complement(2983..4086)
                     /gene="ribD"
                     /locus_tag="ACHJ7J_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO5002163.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQHNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4090..4539)
                     /gene="nrdR"
                     /locus_tag="ACHJ7J_003993"
     CDS             complement(4090..4539)
                     /gene="nrdR"
                     /locus_tag="ACHJ7J_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO5002164.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4690..5229
                     /locus_tag="ACHJ7J_003994"
     CDS             4690..5229
                     /locus_tag="ACHJ7J_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5002165.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDTLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLDFVRIHDIQPVMH"
     gene            5528..6412
                     /gene="tsx"
                     /locus_tag="ACHJ7J_003995"
     CDS             5528..6412
                     /gene="tsx"
                     /locus_tag="ACHJ7J_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5002166.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6450..6797)
                     /gene="yajD"
                     /locus_tag="ACHJ7J_003996"
     CDS             complement(6450..6797)
                     /gene="yajD"
                     /locus_tag="ACHJ7J_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO5002167.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6926..7897)
                     /gene="secF"
                     /locus_tag="ACHJ7J_003997"
     CDS             complement(6926..7897)
                     /gene="secF"
                     /locus_tag="ACHJ7J_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO5002168.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(7908..9755)
                     /gene="secD"
                     /locus_tag="ACHJ7J_003998"
     CDS             complement(7908..9755)
                     /gene="secD"
                     /locus_tag="ACHJ7J_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO5002169.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(9783..10115)
                     /gene="yajC"
                     /locus_tag="ACHJ7J_003999"
     CDS             complement(9783..10115)
                     /gene="yajC"
                     /locus_tag="ACHJ7J_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO5002170.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10138..11265)
                     /gene="tgt"
                     /locus_tag="ACHJ7J_004000"
     CDS             complement(10138..11265)
                     /gene="tgt"
                     /locus_tag="ACHJ7J_004000"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO5002171.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11321..12391)
                     /gene="queA"
                     /locus_tag="ACHJ7J_004001"
     CDS             complement(11321..12391)
                     /gene="queA"
                     /locus_tag="ACHJ7J_004001"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO5002172.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            12484..13065
                     /gene="acpH"
                     /locus_tag="ACHJ7J_004002"
     CDS             12484..13065
                     /gene="acpH"
                     /locus_tag="ACHJ7J_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO5002173.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13086..>13975)
                     /locus_tag="ACHJ7J_004003"
     CDS             complement(13086..>13975)
                     /locus_tag="ACHJ7J_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002174.1"
                     /translation="HIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIAKAGFITGKVNA
                     EKFNVKEFKDDITSMLCEQPPLRTHQISSSGYVSADKLPKLYELNLKDAQRDLANTPV
                     DINGVSLELKTRRHLADIIHVKCMREKNYTTDQGFELPEEVIHALLLDYSDEQQIILR
                     PFFQSAHMDCLFDAFCQELHNISRDGSPRYADLIEQIGSNSEKLQDFDQQLFWRTFTG
                     GVLHRHISVKSEPEKTDVWRFSSQFDFPHINENGELSRTVLMFEGVAESNFKDIANHS
                     SECFSYRCYAAPLDANAKK"
BASE COUNT         3484 a         3726 c         3355 g         3410 t
ORIGIN      
        1 actaacaaaa aatgattatt tgaagaaagt acctttaact gcacaattaa ttgcaaaata
       61 caaaatggat gatcaattgg accaactttt ggttacccgt gaaattcaag atgaaataaa
      121 atcaaagatt caagacaaaa tagatgaact ttctaaaaat ttattcaaca ctatgaccga
      181 aaccattgag aataattttg atgatatttt tcgtcaacag tcagagaata tgagcaatta
      241 ttatgagttc gtcgactgac gatatttcag tgtcattgaa ttatgagtga caaagataca
      301 ccttgaaata tcgccgcata gtatattcgg cgatgtcggg ttttacttta acagagtgcc
      361 ccaaaattgg ggcactgttc atttataaat caaaccgggt ggtctggaat cgcctacacc
      421 tcccggtcaa tggcatttaa agaataccca gcggccagtg atgaccgata aagtacagga
      481 tgcctgcgga aatcaccccg gcgacaatat catcgatcat gatccccatg ccgccatgta
      541 cattgcgatc aaaccagcgg atcggccacg gcttccacat atccagaata cggaaaatca
      601 caaacccggc ggcgacccac tgccagtcat tggtcggcag cgccatcagg gtgatccaca
      661 tgccaataaa ctcatcccag acgatgctac cgtgatcgtg cacacccatg tctttcgccg
      721 tttgatgaca aagatagacg ccgatacaga tccccaacat caccaccagc gagtagagct
      781 gccagggcaa aaaggtcatc agataccaga acggaatcgc cgccagcgat cccatcgtgc
      841 caggaacgat tgggcttaat ccacttccga atccgacagc aagcagatgc cacggattac
      901 tcatcttcag gcgacttttc gcgacatctt tatggcgtgg caaaatggtc atatcctttc
      961 cagtctaacg tgacaggttc gccgtcacga ataaaacaaa gcccttcgat atcggcggtc
     1021 atttgcccga tacaggtaaa cggtacgccc agatgaccga gagccacatc cagcgcgcca
     1081 cggttcagtt ccggcaccgt gaaacacaac tcgtaatctt caccgccaga gagcgcccag
     1141 cgcagcgcct gttccggtac aacatggcga gaaagcgcat cagaaaacgg cagcaatgcc
     1201 aggtcaatac gtgcgccgca gtcgctggct ttcacgatat gcccgagatc ggaaatcaga
     1261 ccgtcagaga gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata
     1321 cgcggcgatg gacggagatg acgtttgatc aagtagtccg catctttagc atcggcaacc
     1381 tgcaaacggt tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt accggtcaca
     1441 tagatccagt cacccggttt cgccccagag cgcgttaagg ctcgtcccat cggaacaaag
     1501 ccgtggatac ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc
     1561 atatcgtaat aattgagaag atcaaacaaa ctgtcgctga aggactcaag ccacgcttcg
     1621 tctacgtccg gtaaggttaa tgccagcgtc agccaggccg gatcggcccc catcgctgcc
     1681 agatcgctta ggttcaccgc cagtgcttta taagccagat cagcaggatc gatatcaggg
     1741 aggaaatggt tgcccgccac cagcgtatca gtgctgatcg ccagggtctg tttctcgggg
     1801 atattgagaa gagcgcaatc gtcgccgatg cccagttcga catcaagacg agaacttctt
     1861 acacggtcaa aataacgggc aatcagggag aactcgccac atgccatacg ttatgcctca
     1921 gcaggtaaaa aagaaaaggc cggcgatcat ggaacggtct tccgtgaatc taccggcctg
     1981 gatattactt tttgttaggg cgaatcacag gtgctgcttt atcgagtacg ccgttgacga
     2041 acttatggct gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca
     2101 ctttgtatgg cacatcgcta cgtttagaca gttcgtacag cgcaatgcgc agtactgctt
     2161 tttctacctg tcccagttct tccagcaggc gggacaggta tggcttcatc agtccgtcga
     2221 ggtatgcggt attagtcgcc accccggcca gcagctcacg gaagtacagg acgtcaacgt
     2281 cttttacatc ctgttcagcc aggaactggt attcaacatc agcgatgtcg ttctgggaca
     2341 actgccagga gtagagcgcc tggacggcac actcacgagc gcggcgacga gcagcaggtt
     2401 tcacggattt ccccttacta atttcaggcc ttgatggctt tcaatacatt aatcatttca
     2461 agcgcggtca gtgcagcttc tgcacctttg ttgccagctt tggtgccagc acgttcgatc
     2521 gcttgttcaa tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg
     2581 gcaacatgcg ccaggccgtt gcttgcacca ccagcgacat attcaaaatg ggcagtgcca
     2641 ccacgaataa ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt agccagtgca
     2701 cccgccgcca gcggcagctc ataggcacca ggcacccaaa caacggtaat gttttcatct
     2761 tttacctgac cgatacgttt cagtgcatca attgcacctt ccagcaggct gtcattgata
     2821 aagttgttga aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc aacgttagct
     2881 tcaataatgt tcattttctt ccttcgggtt cgagtatggc cccgcagggg ggcggatttt
     2941 agcataatat ttcgtgcgct gcttcccttt cgagccggga gatcatgcac ccactaaatg
     3001 caggcaaaca tccgggccta catgacgtat ctctttgaat ttaaattggg gggcgtcagc
     3061 taatttctca agccctggca gtgtgcataa tccacgggcg tcgctgccta atagtttagg
     3121 tgcgatatag acaatcagct catcgactaa acccgcctgc agcaatgcgc cagcgagcgt
     3181 tggccccgct tccacccaga tgctgttaat ctgctgttta cccagttgca tcatcagtac
     3241 aaccagatcc agatgacctt tatgctctgg aatcagcaag gtacgcaccg tttccggcca
     3301 ctcacgagaa tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgtg
     3361 atgttccggc gtcacgcgat tttggctatc aatcacaata cgtaccggct ggcggagatt
     3421 atgttgcggg tagagcgcct gagtttgttc atccagttca gaccaacgca ccgttaaggc
     3481 aggatcatcc gccagcaccg tagcgctgct ggttaaaatg gcatgacttt gtgcgcgcag
     3541 tcgttgtacg tcgcgccgcg cctgaggcga agtgatccac tggctttcgc cgctcgccat
     3601 cgccgtgcgg ccatcaagcg atgcgccaag ttttaactga atataaggaa agccggtgcg
     3661 catccgcttg agaaagcctt tattcaattg ctcggcttca ctcatcatca gcccgtggct
     3721 gacgtcaatg ccagcctgtt gcaaacggta aagtccacgc ccagcgacct gcgggttcgg
     3781 gtcctgcatc gcagcgacca cgcgcgctac gccagcggcg atgagtgcgt cacagcacgg
     3841 tggcgtacga ccatgatggc tacagggttc gagtgtgaca taagcggtcg cacctttggc
     3901 tttttcaccc gccatacgca acgcgtgtac ttcggcatgt ggttcacccg ctcgttggtg
     3961 gtaaccttca ccgacaattt cgccatcttt gacaatgacg cacccgacat tcgggttggg
     4021 atgcgtggta aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt aatactcgtc
     4081 ctgcacggct tagtcctcca ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa
     4141 actgcggtag acagaggcaa aacggatata ggcgacttta tcgagctttt tcaattgctc
     4201 catcaccaga ttgccaatca tcttgctcgg cacttcgcgc tcaccggtgg cgcgcagctg
     4261 cgatttaata tgattgattg ccatttcgac gtcatcggaa ctcaccggac gtttttccag
     4321 cgcccgcagc attccgctac gtaatttttc ttcattaaac ggttcacgca cgtcgttgct
     4381 ttttacaaca cgcggcataa ccagctccgc cacttcaaag gtggtgaaac gttcattaca
     4441 caccagacac tgccgacggc ggcgtacgga tgaaccctcg cccacgagac gagagtcaat
     4501 taccttagtg tccacggcga aacagaatgg gcaatgcata cggcgtcctg accaggtggt
     4561 taacagaaat ctattttacc ctgaactgac gatacaacaa aggcgcaacg cttttgagat
     4621 aaaggatcga tgctttcgct gcttttaccc tctaccatta ggctaattgc gacgagaaag
     4681 ggacgaccta tgaatactaa cgtttttcga ctgctcctgc tgggaagcct gttcagcctt
     4741 agcgcctgtg tgcagcaaag cgaagtgcga cagatgaaac acagcgtcag cacgctgaac
     4801 caggagatga cgcaactcaa tcaagaaaca gtcaaaatca ctcagcaaaa caggctgaat
     4861 gcaaaatcca gcagtggggt ttaccttctg cctggagcga aaacaccggc aagactggaa
     4921 agccagatcg gtactttacg tatgtcgctg gtgaatatta cgcctgatac agatggcacc
     4981 accctgacgc tacgtattca gggcgagtcg aatgatacct tgcccgcatt cagcggaacc
     5041 gttgaatatg ggcaaattca gggaacaata gacaactttc aggaaatcaa tgtgcagaat
     5101 caattgatta atgcccctgc cagcgtcctc gcccccagcg atgttgatat tccgttacag
     5161 ttaaagggga tttctgtgga tcagttagac tttgtacgta tccacgacat tcaacctgtt
     5221 atgcattaaa cgtattgcgg ggcaattttg tgcgtcccgc aacatctttc cccgtcattt
     5281 tgttactccg cttacatcac ccagattgat agtaaaagtt tgcaacaagg gtgaaagtca
     5341 gtacaatccc tgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa
     5401 ctgtgaaacg aaacatattt ttgtgagcaa tgatttttat aataggctcc tctgtatacg
     5461 aaatatttag aaacgcaatt tgcgcctttt tcactcccgc aagggatttt caaacagtgg
     5521 catacatatg aaaaaaacat tactggcagc cggtgcggta ctggcgctct cttcgtcttt
     5581 tactgtcaac gcagctgaaa acgacaaacc gcagtatctt tccgactggt ggcaccagag
     5641 cgttaacgtt gtcggaagct atcacacccg tttcggaccg cagatccgca acgataccta
     5701 ccttgagtac gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc
     5761 gccggtattc ttcggcggta actccgatgc aaaaggtatc tggaaccacg gttctccgct
     5821 gtttatggaa atcgaaccac gtttctccat cgacaagctg accaatactg accttagctt
     5881 cggtccgttc aaagagtggt acttcgcgaa caactacatt tacgacatgg gtcgtaataa
     5941 agatggtcgc cagagcacct ggtacatggg tctgggtacc gatatcgaca ctggcctgcc
     6001 gatgagcctg tccatgaacg tctatgcgaa ataccagtgg cagaactatg gcgcagcgaa
     6061 cgaaaacgag tgggacggtt accgtttcaa aattaaatac tttgtgccga ttaccgatct
     6121 gtggggcggt cagctgagct acatcggctt caccaacttc gactggggtt ccgatttagg
     6181 ggatgacagc ggtaacgcaa tcaacggtat taagacccgt actaataact ctatcgcttc
     6241 cagccatatt ctggctctga actacgatca ctggcactac tctgtcgtag ctcgttactg
     6301 gcacgacggt ggtcagtgga acgacgatgc agaactgaac ttcggcaacg gcaacttcaa
     6361 cgttcgctct accggctggg gtggttacct ggtagtaggt tacaacttct gattgtgaga
     6421 atgccggatt tcatatccgg cattttttat cacttcttct tgttcatcat cgctttcagg
     6481 tcagcgaatg ggttgtactt cgcttcaccg acatctttct gcgcatcttc ccctgcgata
     6541 acggtcgtac catactgatc cgcttcggta tatttcgaat gctcatgatc gtggcaatag
     6601 agacaaagca attcccagtt actgccatct tccgggttat tggtatggtc gtgatcaatg
     6661 tggtgaacgg taagttcacg caggttggaa taaacaaact cgcgggaaca gcgaccgcag
     6721 acccacggat agatttttaa tgctttttcg cgatagccgc tttctaaccg cgcatagttt
     6781 tttgggatga tagccatgaa aagttacctg gatgaaatgt agggttacgc gcagtttccc
     6841 aagatgcggg gattttcgca atctccatac atcaaaaaaa atcccgatct tctgaccggg
     6901 attttcaaca tcaacgggaa attgattaag gcagaattga cggctgatcc gccccttctt
     6961 tttccacttt ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga gccaacgcgg
     7021 atgccacata gatggaagat gcagtaccga tggaaacacc gataagcatg gtcagcgaga
     7081 agccttccag taccggacca ccgaagagat acagcatcag gataaccatc aaggtagtac
     7141 cggatgtgat caaggtacgg tgcagcgtct gggtcaagga cacgttaaag atttcgtaag
     7201 gcgtaccgcg acggatcttg cggaagtttt cacgaattcg gtccgatacc acgatactgt
     7261 cgttaagcga gtaaccgata accgacatca acgatgccac aatggtcagg tcaatctcga
     7321 tatggaataa cgacaaaata cccagcgtaa tgataacgtc gtgcgccagc gcaataacca
     7381 cccctgccgc cagtcgccac tcaaagcgga aacctacgta cacgaggata gacagcagcg
     7441 ctgccatcaa cgccatcgca ccggtttgcg caaggtctgc ccccacgctc ggaccgacga
     7501 actcaatacg cttcactgct gcattctgat tggtggattc gttaatcacc ttcagaacct
     7561 ggctgcccag cacctgaccg ccggtttcgc cttcagcagg cggcatacgg accatgatgt
     7621 cgtggctgct accaaagttt tgcagcattg gctcttcaaa accggctttt tgcaatgcat
     7681 cacgcattac gtcaatttca gccggttttt cgagcgtaat ttcgataacc gtaccaccgg
     7741 tgaaatccag cccccagtta aagccgcgca cgcccataat aacgatagca gcgattaaca
     7801 gcagaccaga gatgccgaaa gcccagtagt cccagcgcat aaagtcatag actttacggc
     7861 cgtggtttag ttgttcaaca gtatattcct gtgccacatc gcactcctca gattgacagc
     7921 ttcttgacgc gcttgccgcc atatagcagg tttacgatgg cacgcgtacc gacaatcgcg
     7981 gtaaacatcg acgtcgccac accgataccg gtagtaatcg cgaacccttt aattgccccg
     8041 gtacccactg cgtacaggat gatgacttta atcagcgtgg tgatgttcgc atcgaagata
     8101 gaactgaatg cgccacgata accttcatca attgcctgtt gaacagtacg tccgttgctc
     8161 aactcttctt taatacgttc gttgatcagt acgttcgcat cgaccgctac cgcaagggtt
     8221 aagacgatac ccgcgatacc tggcatactc agtgttgcgc ctggcaacag cgacataata
     8281 ccgacgatca agatcaagtt ggcaatcaga gcactggtcg caatcagacc aaacttctta
     8341 tagaagatga tcatgaacag aatagaaacc agcagaccgg caaggcaagc ctccagcccc
     8401 tgctcaatgt tctgcatacc cagggtcggg ccaatggtac gttcttcaac aatctgaatc
     8461 ggcgcgatca acgcaccggc acgcagcagc agtgacagct gacgggcttc gttcgggttg
     8521 ttgatgccgg tgatacggaa gctgttaccc agacgagact ggatgttggc gatgttaatc
     8581 acctcttcct gtttcaccag aaccgcacga ccatttgcat ctttcttacc gctgtctttg
     8641 tactccacaa acagggttgc catcggtttg ccgatgttgt ccttggtgaa gttagacata
     8701 atgttgccac cagcgctatc gagcgagatg ttaacctgcg gctggttgta ttcgtcctgg
     8761 ctggaagtgg agtcggtgat atggtcaccg gtcagaatta cgcgtttgta cagcacaacc
     8821 ggctgacctt cgcgggtctg tttcacttca gagtcgcccg gtacgcgacc ggatgccgca
     8881 gcagcctgat caacgttggt gtttaccaga cggaattcca gcgttgccgt cgcacccaga
     8941 atctctttcg cacgcgcagt gtcctgaata cctggcagtt caacaacgat acggtcagca
     9001 ccctgacgct gaaccaccgg ctccgccacg ccaagttggt ttacacggtt acgcaggata
     9061 ttgatgttct gctgcaccgc atattcacgc gcttcactca gacgagcatc gctcattacc
     9121 gcacgcagct ggttgctgcc ctggctacta atcaccaggt ccggatggcg cttgctcaga
     9181 tacgcaatgg cttcatcacg agctttagca tcgcggaaag tgatgctcag gccgtagttg
     9241 ttttctttac gaacggtggt atacgggatg cctttttcgc gcaggtcgct gcgcaggcta
     9301 tcgatatttt gttcctggag tttgccaagc gcggtgtcca tatccacttc catcaggaag
     9361 tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct cagcgtgaat agctgccagc
     9421 cagcgcggcg ttgccggggc aaggttaagc gccacgacgt atttgtcacc cataacgccc
     9481 attaatgctt cacgagcgcg cagctgggtg tcagtggagt cgaagcgcgc aagaatagcg
     9541 ccctcttcca gtgccacaga cttagcagtt attttttctt cttgtaacgt tttctggacc
     9601 tggatcagcg tttgctcact ggcggcgact ccgcgcgcac cagtgatctg aacagccgga
     9661 tcctcaccaa acaggttggg aagcgcatac agcagaccga tgacaatcac cacgatcagc
     9721 atgacgtact tccacaaagg ataacggttt aacacggcaa ttcccttagg gaaaaatttt
     9781 aattacagcg ccttcatggt gcctttcggc aggacggcag ctacgaagtc acgtttaata
     9841 actacttcag tggtgtcatt cagcgcgata gcaatgtagc cgttttccgc tactttggtt
     9901 acgcgaccga ccaggccacc gttcgtcaga acttcatcac ctttggcaat ggagtccatc
     9961 agctttttgt gttctttggt gcgcttctgc tgtggacgca ggatcatgaa atagaaaatc
    10021 agaccgaata ccaccagcat caaaatcaaa gacatcgggc taccttgcgc cggtgcaccc
    10081 gttgccgcta ccgcatcaga aataaaaaag ctcattaaat ttccctcatt attaatatta
    10141 atcaacgttc aaaggtggta cttctcgccc ctgacgctgg taaaaatcag ttacgaagct
    10201 ctctaattta ccctcttcaa tagccttgcg taaacccgcc atcaaacgct ggtagtaacg
    10261 aaggttatga atggtattga gtcgcgcgcc taatatttcg ttgcaacggt caagatgatg
    10321 caagtaagcg cgtgaataat tgcgacaggt gtagcaatca cactcaggat cgagtgggcc
    10381 agtatcgctc ttatacttcg cattgcggat tttcaccacg ccatcggtca cgaacaaatg
    10441 accatttcgg gcgttgcggg ttggcattac gcagtcaaac atatcgatac cacgacgtac
    10501 gccttcaacc aggtcttctg gtttaccaac gcccatcagg taacgcggtt tgtctgccgg
    10561 aatttgcggg catacatgct ccagaatgcg gtgcatatct gctttcggct cacccacagc
    10621 cagaccgccg acagcgtagc catcaaaacc gatatctacc agacctttaa cagaaatatc
    10681 acgtaaatct tcgtaaacgc tgccctgaat gataccaaac agcgcatttt tgtttccgag
    10741 actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga gacatctcca tggagcgttt
    10801 tgcgtaatcc cagtcagcag gatacggcgt acactcatca aagatcatga cgatatccga
    10861 accaagatcg tactgaatct ccatcgactt ttccgggtcg aggaaaatcg ggtcgccgtt
    10921 gatcgggtta cggaagtgca cgccctgttc ggtgatttta cgaatatcgc caaggctgaa
    10981 gacctggaag ccgccggagt cggtgaggat cggtcccttc cactgcataa aatcgtgcag
    11041 atcgccgtgc agtttcatga tttcctggcc cgggcgcagc cacaggtgga aggtgttgcc
    11101 gaggataatt tgcgcgccag tggcttcaac ttcttctggc gtcatccctt ttacggtgcc
    11161 gtaggtgcca acaggcataa aacaaggcgt ttccactacg ccacgatcaa agaccaggcg
    11221 gccacggcgt gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt ctccactacg
    11281 tcagaaaaac agtccaacgt tttaaaccag cgccgcggaa ttactccccg acgcgctcat
    11341 taattgcctg cggattgtac gtgataaaca tcgcatcacc gtaactaaaa aagcgatatt
    11401 tctcttctac cgctgcttta taggcgttca tggtgtgttg ataaccggca aaggccgaaa
    11461 ccagcataat cagcgtcgac tctggcaagt ggaagtttgt caccagcgca tcgaccactt
    11521 tgtactggaa gcccggatag ataaagattt gggtatcgtc aaagaacggt tcgatgagat
    11581 cgtttttcgc cgcctgagcc gcgctttcca gcgaacgtac tgaggtggtg ccaaccgcaa
    11641 tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc taccacatcc tgcggtactt
    11701 cagcgtattc cgagtgcatg atgtgatctt caatggtgtc gacgcgtacc ggctggaagg
    11761 tgcccgcacc aacgtgcagg gtgacgaacg ccatctccac gcctttggcg cgtaattttt
    11821 ccagcaaagg ttcgtcaaaa tgcagaccag cggtcggggc tgcaaccgcg cccggttttt
    11881 cgctataaac ggtttgataa agttcgcggt cagcgtcttc gtccggacgg tcgatatacg
    11941 gcggcagcgg catatggccg atgctgttga gaatatccag caccgaacgc tcatcattaa
    12001 attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt tgcgttaata ctttcgtcat
    12061 cgcccagcag cagttctgcg ccaggttttg gcgctttcga ggcgcgaata tgcgcaagaa
    12121 tgcgtttgtc gtcgagcatc cgttcaacca gcacttcaat cttgccgccg ctggctttac
    12181 gcccaaacag gcgcgccggg atcacccggg tattattaaa aaccagaaga tcgccggggt
    12241 tgagcttatc gagtaaatcg gtgaaagtac cgtgcgtcag cgcgcccgtc ggcccgtcca
    12301 gcgacagtaa acgacagcta ctgcgttcag gcatcggata gtgggcaatc agggattcgg
    12361 gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac tcagactaaa ataagaggcg
    12421 ggtagtctag tgccggggcg ctctccctgc aacctttacc cccctttgga tagaatataa
    12481 tcgatgaatt ttttagctca cctgcattta gcccatctcg cggaaagctc gctttccggc
    12541 aatttgctgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc cgacgtcgtg
    12601 gctggcattc atatgcatcg acgtatcgac gtattgactg acaatctgcc ggaagtccgc
    12661 gaagcacggg agtggtttcg tagtgaaact cgccgcgttg cgcctattac gctggatgtc
    12721 atgtgggatc actttctttc ccgtcactgg tcgcagctgt cgccggactt tccgctacag
    12781 gaatttgttt gttatgcccg cgagcaagtg atgacaattt tgccggactc accgccacgc
    12841 tttatcaacc tgaacaacta cttatggtca gagcagtggc tggtgcgcta tcgcgatatg
    12901 gatttcatcc agaacgtgtt aaacggcatg gcaagccgtc gcccacgtct ggatgccctg
    12961 cgtgactcct ggtacgattt agacgcccat tatgccgccc tcgaaacccg cttctggcag
    13021 ttttatccac ggatgatggc gcaggcgtca cgcaaggcgc tctgaaactt caaactccgg
    13081 cgtcactatt ttttagcatt cgcatccagc ggggcggcat aacaacggta agaaaaacat
    13141 tcgctggaat gattggcgat atctttaaag ttgctttcag caaccccctc gaacatgagt
    13201 accgttctgg acaactctcc gttttcattt atatgtggaa aatcaaattg gctactgaat
    13261 cgccatacat ctgtcttttc tggctcgctc ttcacagaga tatgtcgatg gaggacccct
    13321 cctgtaaacg tgcgccagaa caattgttga tcaaaatctt gtaatttctc agagttggat
    13381 cctatttgct ctattaaatc tgcataccgc ggactgccat cacgagaaat attatgcaac
    13441 tcctgacaaa aagcatcaaa caaacaatcc atgtgagcac tttggaaaaa gggtcttaat
    13501 atgatttgtt gctcatcgct gtaatccagg agcagggcat ggattacttc ctctggtagt
    13561 tcaaatccct gatcagtggt ataatttttt tctcgcatgc attttacatg aataatatca
    13621 gctagatgcc tccgggtttt taattctaaa ctaacaccat tgatatcaac cggagtgtta
    13681 gccagatctc tctgcgcgtc tttcaaattt aactcataga gtttgggcag tttatctgca
    13741 gatacgtatc cggatgaaga aatctggtgt gtacgcaggg gcggctgttc acaaagcatc
    13801 gaggttatat cgtctttaaa ctcttttaca ttaaacttct cggcattaac cttccctgtt
    13861 ataaaccccg ctttggcaat tttcttctct aattgttttg tacgtttaga ataataagcg
    13921 ttgttatcat aaaacttttc aagaagggcc cactcctcaa gttgccttat gtgat
//
LOCUS       DAZFFG010000120        13925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFFG010000120 DAZFFG010000000
VERSION     DAZFFG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.120"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..511)
                     /locus_tag="ACHJ7J_004004"
     CDS             complement(<1..511)
                     /locus_tag="ACHJ7J_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFO5002175.1"
                     /translation="MLIIDQSDITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSIFQMNRDTTITAGILLKQLDNVTQIVQHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKT"
     gene            complement(609..1967)
                     /gene="dgcT"
                     /locus_tag="ACHJ7J_004005"
     CDS             complement(609..1967)
                     /gene="dgcT"
                     /locus_tag="ACHJ7J_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5002176.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQMIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVLG
                     GPIVAHILSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNVSLLFMSRYSVSIWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKDIAHRDPLTNICNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCD
                     SP"
     gene            2556..4979
                     /gene="pgaA"
                     /locus_tag="ACHJ7J_004006"
     CDS             2556..4979
                     /gene="pgaA"
                     /locus_tag="ACHJ7J_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO5002177.1"
                     /translation="MYSSSRKRCPQTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKQAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQIDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTSRELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGVVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTELDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            4988..7006
                     /gene="pgaB"
                     /locus_tag="ACHJ7J_004007"
     CDS             4988..7006
                     /gene="pgaB"
                     /locus_tag="ACHJ7J_004007"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO5002178.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSKTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVD
                     REYFATWQQVREVARSPLVEVSSHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     AGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVTQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            6999..8324
                     /gene="pgaC"
                     /locus_tag="ACHJ7J_004008"
     CDS             6999..8324
                     /gene="pgaC"
                     /locus_tag="ACHJ7J_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO5002179.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYVVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            8326..8739
                     /gene="pgaD"
                     /locus_tag="ACHJ7J_004009"
     CDS             8326..8739
                     /gene="pgaD"
                     /locus_tag="ACHJ7J_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO5002180.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(8789..9577)
                     /gene="phoH"
                     /locus_tag="ACHJ7J_004010"
     CDS             complement(8789..9577)
                     /gene="phoH"
                     /locus_tag="ACHJ7J_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO5002181.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVHSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(10198..11469)
                     /gene="efeB"
                     /locus_tag="ACHJ7J_004011"
     CDS             complement(10198..11469)
                     /gene="efeB"
                     /locus_tag="ACHJ7J_004011"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO5002182.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(11475..12602)
                     /gene="efeO"
                     /locus_tag="ACHJ7J_004012"
     CDS             complement(11475..12602)
                     /gene="efeO"
                     /locus_tag="ACHJ7J_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO5002183.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(12660..13490)
                     /gene="efeU"
                     /locus_tag="ACHJ7J_004013"
     CDS             complement(12660..13490)
                     /gene="efeU"
                     /locus_tag="ACHJ7J_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO5002184.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESIFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSEVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3808 a         3328 c         3113 g         3676 t
ORIGIN      
        1 ctgttttacc tggcacaaaa cttgtaccgc taattgatac tatacgtgat tctttatttt
       61 tattgtgaaa gctcgtaccg taaacatcgt taacattcat ttttttgaaa ccgctaactg
      121 aagaacatat ttggaaatta ttttctataa gacccgttgt tcgaacatag ggagtcaatg
      181 ctccgttagc aatcaactgt tcaagaatgt ctttgcaggg cttcatcacc agaatagagg
      241 taagctttgt cgtatgctgg acaatttgcg taacattatc aagctgtttt aacagaatac
      301 cggcggtaat tgtcgtatct ctattcattt gaaatattga catagcaaac gatagtaaca
      361 cccccagaat gcctggtatt ataatgagta caaacatttt tttgttgaat accatacatg
      421 tttccccacg catcagggtg atgcaaccta agaaattata gtacaattgc cagtaaaagc
      481 gggtgatatc agactgatca atgataagca tttaagatca tatagtacta tgatatttct
      541 gaaaattgtt acgcatgtct gcgaatagct atttagcgct taccaaaatc acccgctaaa
      601 atgccgcttt atggtgaatc acaaaaattc accacatccc tcacaaccac cttattacgc
      661 ccggtctctt tcgcctcgta tagtgcgtta tcagccagtc gatagatctc attcgggttt
      721 aacgcatttt cctgagtcac aacagcgcca atactaatcg tcactttttg tggtatggcg
      781 tattcaggat tatcaccagt taaacgctca acattttccc gaatcctttc cgctaaagct
      841 ttcgcacgtt cagtatcgat gtccgtcagc aagacgccaa actcttcgcc gccgacgcgc
      901 gcaaaaaggt catctggccg tatgcttttc ccaatgatat tcaccactgt ttttatcacc
      961 tgatcgccaa ccggatgccc ccaggtgtcg ttgacttttt tgaagtggtc gatatccata
     1021 atcatgacgc aataaggcgt ttttttggcc gatgctgatt gaaccgtcag ttcattaaaa
     1081 aaataatttc tgttacatat attggttaag ggatcgcgat gtgcaatgtc ctttgttact
     1141 cgtagcgcac tgaatatatg gcacataaat attaccataa cggttaactt actgactact
     1201 tcaatagtac gactaatata ccaaatggac acgctatatc tgctcataaa taaaagagag
     1261 acattgtaca gtacggcaca aaaagcaatc accgtcactc cattccagat atcatagcgt
     1321 aaaccattaa agtatagatt aaccgaaagc aaggttaacc acatgaaaat cattatggta
     1381 acatatgaag ctttccaaac cacttgacca ttttcattgg ttaactcggc aatgtgtaaa
     1441 tcgtagctct cataatggct agaaagtatg tgagcaacta tcggcccccc aagaacaaaa
     1501 aacaaactta tgcacaaagt catttttttg gaaaataaat tgcgctgttt tgtgttttca
     1561 ctaactttat tcaccaatgc cagaaatatt aatatgcaca aactcatctg acgaaacaaa
     1621 taataaattg caatgtcatt ttgaacaaca ctgcttgtca aacgcccctc aatcatttgc
     1681 tgaatgataa tgacggtttt aacaaagtaa atcaaacaac tcagaaaagc caaactaagt
     1741 atacagtttg aaaattgcct acggtcataa atgaaataca actgcataaa aataaatgca
     1801 acagtatcga taaaaaataa agcaatcatt accatcagat atgttgatcg tggaacaact
     1861 tcctcaacgc ctggctgatt aaaccaacta ttcaccaaaa ccaaagcaag cccaaacaat
     1921 aagctcacta agacagtact actaattctc aaatagtctt tttccataaa gctacaccaa
     1981 cctgtaggtt gtagatccct ttctattatt aatagatata agaactgtac tgttgtcaat
     2041 atgaaattcc gcccacaaac aataccgatg atgatagttg cagaccgcaa ttattattac
     2101 cttagtgctt tatacattct ttgcgacaag acaggcttgc aaatatttat aaatggttat
     2161 aaggaaatcc ttataaaaca ttcattcata gatatattag gaataacaat tgaatcggtg
     2221 agtacagtaa cgctgcattt catcaggact ttcgaaaaat ccgaaatcat gcatcggaat
     2281 ttactgattt aattatttta ctcctaactt attttgaaaa aggcattggg atttatgccg
     2341 tattcctgaa gatcctcatc attggaatgg attttcgggc gagaaaagga ttttatatgg
     2401 acactctgct catcatttct tcttctcatc atcaacaatt cacgtctctc ttccgcgttt
     2461 aataacggat tatgaggtgc aaaaaatatc tttcttttca gttacctgtc attagataca
     2521 gagagagatt ttggcaatac atggagtaat acaggatgta ttcaagtagc agaaaaaggt
     2581 gcccgcaaac caaatgggct ttgaaaattc ttactgccgc atttttagca gcgagtcccg
     2641 cggcgaagag tgccgttaat aacgcctatg atgcattgat tattgaagct cgcaagggaa
     2701 atactcagcc agctttgtta tggtttgcac aaaaatcagc actcagtaat aaccaaattg
     2761 ctgactggtt acagattgcc ttatgggccg ggcaagataa acaggttatt accgtttaca
     2821 accgctaccg ttatcagcaa ttaccagcgc gtggttatgc agctgtcgcc gtcgcttatc
     2881 gtaacctgca acaatggcaa aactcgctta cactgtggca aaaggcgctc tctctggagc
     2941 cgcaaaataa ggattatcaa cggggacaaa ttttaaccct ggcagatgcg ggtcactatg
     3001 atactgcgct ggttaaactt aagcagctta actctggagc accggacaaa gccaatttac
     3061 tcgcagaagc ctatatctat aaacaggcag ggcgtcatca ggatgaatta cgggcgatga
     3121 cagagtcatt acctgaaaat gcatctacgc aacaatatcc cacagaatac gtgcaggcat
     3181 tacgtaataa tcaacttgct gccgcgattg acgatgccaa tttaacgcca gatattcgcg
     3241 ctgatattca tgccgaactg gtcagactgt cgtttatgcc tacgcgcagt gaaagtgaac
     3301 gttatgccat tgccgatcgc gccctcgccc aatacgctgc attagaaatt ctgtggcacg
     3361 ataacccaga ccgcactgcc cagtaccagc gtattcagat tgatcatctt ggcgcgttat
     3421 taactcgcga tcgttataaa gacgttattt ctcactatca gcgtttaaaa aagacggggc
     3481 aaattattcc gccctgggcg caatattggg ttgcgtcggc ttatctcaaa gatcatcagc
     3541 cgaaaaaagc acagtcaata atgaccgagc tcttttatca caaggagacc attgccccgg
     3601 atttatccga tgaagaactt gcggatctct tttacagcca cctggagagt gaaaattatc
     3661 ccggcgcgct aactgtcacc caacatacca ttaatacttc gccgcctttc cttcggctaa
     3721 tgggcacgcc tacgagcatc ccgaatgata cctggttaca ggggcattcg tttctctcaa
     3781 ccgtagcaaa atatagtaat gatcttcctc aggctgaaat gacatccaga gagcttgctt
     3841 ataacgcacc aggaaatcag ggactgcgca ttgattacgc gagtgtgtta caagcccgcg
     3901 gttggcctcg tgcggcagaa aatgaattaa aaaaagcaga agtgatcgag ccacgtaata
     3961 ttaatctgga ggttgaacaa gcctggacag cattaacgtt acaagaatgg cagcaggcag
     4021 ctgtcttaac gcacgatgtt gtcgaacgtg aaccgcaaga tcccggagtt gtacgattaa
     4081 aacgtgcggt tgatgtacat aatcttgcag agcttcgtat cgctggctca acaggaattg
     4141 atgccgaagg tccggatagt ggcaaacatg atgtcgactt aaccaccatc gtttattcac
     4201 caccgctgaa ggataactgg cgcggttttg ctggattcgg ttatgccgat ggacaattta
     4261 gcgaaggaaa aggggttgtt cgcgactggc ttgcgggtgt tgagtggcgg tcacgtaata
     4321 tctggctcga ggcggagtac gccgaacgct ttttcaatca tgagcataaa cccggcgcgc
     4381 gcctgtctgg ctggtatgat tttaatgata actggcgtat tggttcgcaa ctggaacgcc
     4441 tctctcaccg cgttccatta cgggcaatga aaaatggtgt tacaggcaac agtgcacagg
     4501 cttatgttcg ttggtatcag aatgagcggc gtaagtacgg tgtctcctgg gctttcactg
     4561 atttttccga cagtaaccag cgtcatgaag tctcacttga gggtcaggaa cgcatctggt
     4621 cttcaccata tttgattgtc gatttcctac ccagtctgta ttacgaacaa aatacagaac
     4681 tcgatacccc atactacaac cctataaaaa cgttcgatat tgttccggca tttgaggcaa
     4741 gtcatttgtt atggcgaagc tatgaaaata gctgggagca aatcttcagc gcaggtgttg
     4801 gtgcctcctg gcaaaaacat tatggcacgg atgtcgtcac ccaactcggc tacgggcaac
     4861 gcattagctg gaatgacgtg attgatgctg gcgcaacgct acgctgggaa aaacgacctt
     4921 atgacggtga cagagaacac aacttatacg ttgaattcga tatgacattc agattttaag
     4981 gataaatatg ttacgtaatg gaaataaata tctcctgatg ctggtgagta taattatgct
     5041 cactgcgtgc attagccagt caaaaacatc atttataccg ccacaggatc gcgagtcttt
     5101 actcgccgag caaccgtggc cgcataatgg ttttgtggcg atttcatggc ataacgttga
     5161 agacgaagct gccgaccagc gttttatgtc agtgcggaca tcagcactgc gtgaacaatt
     5221 tgcctggctg cgcgagaacg gttatcaacc ggtcagtatt gctcaaattc gtgaagcaca
     5281 tcgaggagga aaaccgctac cggaaaaagc agtagtgctg acttttgatg acggctacca
     5341 gagtttttat acccgcgtct tcccaattct tcaggccttc cagtggcctg ctgtatgggc
     5401 ccccgtcggc agttgggtcg atacgccagc ggataaacaa gtaaaatttg gcgatgagat
     5461 ggtcgatcga gaatattttg ccacgtggca acaagtgcga gaagttgcgc gttccccgct
     5521 cgttgaggtc tcttctcata catggaattc tcactacggt attcaggcta atgccaccgg
     5581 cagcttattg cctgtatatg taaatcgtgc atattttact gaccacgcac ggtatgaaac
     5641 cgcagcagaa taccgggaaa gaattcgtct ggatgctgta aaaatgacgg aatacctgcg
     5701 tacaaaggct aaggtaaatc cacacgtttt tgtttggcct tatggcgaag cgaatggcat
     5761 agcgatagag gaattaaaaa aactcggtta tgacatgttc ttcacccttg aatcaggttt
     5821 ggcaaatgcg tcgcaattgg attccattcc gcgagtatta atcgccaata atccctcatt
     5881 aaaagagttt gcccagaaaa ttattaccgt acaggaaaaa tcaccacaac ggataatgca
     5941 tatcgatctt gattacgttt atgacgaaaa ccgccagcaa atggatcgca atattgatgt
     6001 gttaattcag cgggtgaaag atatgcaaat atcaaccgtg tatttgcagg catttgctga
     6061 tcccgatggt gatgggctgg tcaaagaggt ctggtttcca aatcgtttgc taccaatgaa
     6121 agcagatatt tttagtcggg ttgcctggca attacgtacc cgcgcaggtg taaacatcta
     6181 tgcgtggatg ccggtattaa gctgggattt agatcccaca ttaacgcgag taaaatactt
     6241 accaactggg gagaaaaaag cacaaattca tcctgaacaa tatcgccgtc tctcgccttt
     6301 tgatgacagg gtcagagcac aagttggcat gttatatgaa gatcttgccg gacatgctgc
     6361 ttttgatggc ctattgttcc acgatgatgc tttgctttca gattatgaag atgccagtgc
     6421 accggctatc gcggcttatc agcaagcagg ctttagcggg agtctgagcg aaattcgaca
     6481 aaacccggag caatttaaac agtggacccg ctttaaaagt cgtgcgttaa ctgacttcac
     6541 tttagaactt agtgcgcgcg taaaagccat tcgtggtcca catgttaaaa ctgcacgaaa
     6601 tatttttgca cttccggtaa cacaacctga aagtgaagcc tggtttgcac agaattatgc
     6661 tgatttccta aaaagctatg actggaccgc tattatggct atgccttata tggaaggtgt
     6721 cgcagaaaaa tcggctgatc aatggttaat acaactgacc aatcagatta aaagcatccc
     6781 tcaggctaaa gacaaatcta ttttagaatt acaggcacaa aactggcaga aaaatggtca
     6841 gcatcaggct atttcttcgc aacaactcgc tcactggatg agcctattac aactgaatgg
     6901 agtgaaaaac tatggttatt atcccgacaa ttttctgcat aaccaacctg aaatagacgt
     6961 tattcgtcct gagttttcaa cagcctggta tccgaaaaat gattaatcgc atcgtatcgt
     7021 tttttatatt atgtctggtg ttatgcatac ccctatgcgt agcgtacttt cactctggtg
     7081 aactgatgat gaggttcgtt ttcttctggc cgttttttat gtccattatg tggattgttg
     7141 gcggcgtcta tttctgggtc tatcgtgaac gccactggcc gtggggagaa aacgcaccag
     7201 ctccccagtt gaaagataat ccgtctatct ccattatcat tccctgtttt aatgaggaga
     7261 aaaacgttga ggaaaccata cacgccgctt tagcacagcg ttatgagaac attgaagtta
     7321 ttgccgtaaa tgacggttca acagataaaa cccgtgccat cctggatcgc atggctgcac
     7381 aaattcccca tttgcgggtc attcatctgg cgcaaaacca ggggaaagcc attgcgctta
     7441 aaaccggagc tgccgcggcg aaaagtgaat atctggtgtg cattgatggc gacgcgttat
     7501 tagaccgcga tgcggcggca tatattgtgg aaccgatgtt gtacaacccg cgtgtgggtg
     7561 ccgtaaccgg taatcctcgt attcgaacac gttctaccct ggtgggtaaa attcaggttg
     7621 gcgagtattc ctcaattatt ggtttgatca agcgaaccca gcgtatctat ggaaacgtat
     7681 ttaccgtttc cggtgttatt gccgcatttc gtcgcagcgc cctggcagaa gtgggttact
     7741 ggagtgacga tatgatcacc gaagatattg atattagctg gaagctgcag ttgaatcagt
     7801 ggacgatttt ttacgagcct cgggcgctgt gctggatatt aatgcctgaa acgttaaaag
     7861 ggctgtggaa acagcgcctg cgctgggctc agggcggtgc agaagtattc ctcaaaaata
     7921 tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc actgtttttt gaatactgcc
     7981 tgacgacaat atgggccttc acctgcctgg tcggtttcat tatttacgta gtccaacttg
     8041 caggtgtacc gttaaatatt gaattgacac atatcgctgc gacacatact gccggaatat
     8101 tattgtgtac gttatgttta ctgcaattta ttgtcagcct gatgatcgag aatcgctatg
     8161 agcataatct gacttcatcg cttttctgga ttatttggtt cccggttatt ttctggatgc
     8221 tgagcctggc aacgacattg gtatcattta cacgagtcat gttgatgcct aaaaagcaac
     8281 gcgcccgttg ggtaagtccc gatcgcggga ttctgagagg ttaatatgaa caatttaatt
     8341 attacgaccc gacaatcgcc agtacgttta ctggttgatt atgttgccac aactatcttg
     8401 tggacattat ttgctttgtt catattctta ttcgccatgg atctgctgac gggttattac
     8461 tggcaaagcg aggccagaag ccgacttcag ttctattttt tgctggcagt ggcgaatgcc
     8521 gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt ttcaaaaaca gcagcatcat
     8581 gcagcctacc aatatacgcc gcaagaatat gcagagagct tagcaatacc tgatgagctc
     8641 tatcagcaat tacaaaaaag ccacaggatg agcgtacact tcaccagcca ggggcaaata
     8701 aaaatggtgg tttcagaaaa agcgctagtc cgggcataaa cgcccaaaac aaagcccggt
     8761 tcgcccgggc tctgcaccga taatacactt aactgtatgc atgtagcgta cgttggcaaa
     8821 gtgccgaacg tacgcaatcc tctttaccga accggacgat cccgaccatt tcatcttctt
     8881 cgaaacgttc cagtgcgtca cttaatccgg agtgcacgcc gcgaggtaaa tcgcattgcg
     8941 tgatatcacc gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca
     9001 tttgcgcggc agtcacattc tgcgcctcgt caagaatgac gactgcattt tcaaaggtac
     9061 gtccacgcat ataggcgaac ggcgcaattt ccaccttccc tatttccggt cgcaggcagt
     9121 actgcataaa ggaagcccct aagcgccgga ccagcacgtc gtataccggg cgaaaatagg
     9181 gagcaaactt ttctgcgata tctccaggta agaagccaag atcttcatcg gcttgcagaa
     9241 ctggacgggt gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg
     9301 ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac
     9361 tctcaatagc cttcagatag tgcaattgcg cttcatttcg cgcgaggatg ggcgaggtat
     9421 cgcgactgtc gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga
     9481 ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct
     9541 cgcgacgagc tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt
     9601 attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc
     9661 cttgtaagcc atagtttgct tttggataaa atgccgaaaa acggctacgc gcaccgttta
     9721 cggcgtcggt aacacatgaa aagaaaggat gaggttgaaa atgcaaagtg acgagatgac
     9781 taccggagga gaaaactccg cgagtggtgg cgcgttgatt atctaaaaca tgtccagtac
     9841 aggacgttac catccgcgat ctccatagtg actgactatc actgccggga acttccgctg
     9901 ctacttaata agtacaacag atctggcatt cattgcaaca atatattcac ttatatttaa
     9961 ctataaaaca ccattttagt gacattagtt tctactggaa agatgacaga gtgatgacag
    10021 agatgaaaaa agctgtgtgc tttcagcagg attcacatca ggcatcagtg cacgatcgcc
    10081 ggatgcggcg tgaacgcctg atccagccta ccaaacccgc accgattgtg ggcatcataa
    10141 gcatcgcaag cgccgcatca ggcatctgcg cacaaatgcc ttatccgcct aaaaacatta
    10201 aacccgcaat aatgcgcttc cgagataatc gttcgcgtcc ttcactcccg gcagcgcaaa
    10261 aaaataaccg ccgccgatag gtttaacgta ttcctccagc gcttcgccat tgagcctttt
    10321 ttgtactgtc aggaagcctt tttccagatc gtgctggtag cagacaaaca gcaaccccat
    10381 atccagttgt ccggagttgg tgacgcccag tgaataactg tagccacgac gcagcatcag
    10441 gctggactca ctctccgccg tgcggggatt cgccagccgg atatggctgt ccaacgcaat
    10501 caccttccct tccgggtcgc tggcgtaatc aggcacatca tgctcatgtt gcattcccag
    10561 cggcgcaccg gtttgcttat cacggccaaa aatcgtctgc tgttctttca gcggcgttct
    10621 gtcccaaaat tccactcgaa actgaatcaa gcgtactgcc tgatagctgc caccgattgt
    10681 ccacgcaggc tcctgctgat ctgccgttac ccacaccact ttttgcatca acttatcatt
    10741 ctggctatcg ggattggcag tgccgtcttt gaaacccagc aaattaatcg gcgtctcttt
    10801 gcctttacta cgcgccgcgt gatcggaaat aaacccttcc cgcttccagc gcacactgag
    10861 caaatccggc gtgtgtttga tgatatcgcg cagcgcatgg atcaccgtgt cctgggtgtt
    10921 ggcgcaaatc tgtagcaaca catcaccatg acataacgcc gcatccagcg agtcgttggg
    10981 gaaacgcgtc atcttctgca actttttcgg catctgtggc gcaaggccaa accgctcatc
    11041 aaacaatgag tgacccaccg ataacgtgat ggtgagatta tcgggcgcaa tgtagccgcc
    11101 aagaatgccg gaatcgagtg gtggcaggcg cggatttggc gtttctggtg ccgctccgcc
    11161 ctgagtcaga aaagcaaaac gctgagtcaa caagcgaaac aaccgttcaa gatcggcttt
    11221 atcgctggca agcacatcaa acgccaccag catcattgcg gcctgttgtg gcgtcaggat
    11281 ccctgcctga tgctcaccat aaaacggctg tttctcattg cgtgcatccg gtgaaagcgt
    11341 acccggtgca ctttgcgttt tttgtgcatg agcgaccgga caacttcccg ccagcgccag
    11401 tgcacctatc cctttcagta aacgtcggcg tgacggttca ttcacgccgt tttcatcttt
    11461 atactgcata acgcttaatc cagtcccagc acaccgcgaa gttgcgccag atcttccgcc
    11521 agcgcagtaa tcggcccttt cagcgcgttc cggtcggcat cggtcaattt gtcgtaggtt
    11581 tcaaaaccgt ctttagtacg gtatttcgcc agaatggtat cgaccttttt aaagttggca
    11641 tcgacttttg ccaacagttc cgggttggct ttttgcagtt gtggacgcag cagatcgaca
    11701 attttctgcg agccttcaac gttagcctgg aaatcccaca gatcggtgtg gctgtagcga
    11761 tcttcttcac cgctgatttt gctggctgcc acttcctcaa tcagtccggc tgcgccgccg
    11821 accacttttg aaggtgggaa agccagttca ctgatgcgtt tttgcaaatc gaccacatcg
    11881 gtataaagct gctcagcgta ctgatccatc cctttggtgg tgttgtcgcc aaacaatgct
    11941 ttttccagac ggtggaaacc agtgaatttt gggtcggcgg ctttttgctc gtaatcatct
    12001 tcacgggcgt caatgctgcc atccagatcg gagaacagtt cagcaatcgg ttcaatgcgc
    12061 tcatagtgct ggcgcgtcgg cgcatacagt gctttcgctt tttcgatatc gcctgcttta
    12121 atcgcgtcgg taaaggcttt ggtgtcggtc accagctgtg tggtttccgc catgacatac
    12181 gctttatatg cagtaattgc accaccaaga cttaacagcg catcactttg cgccgcatcc
    12241 gccgttgcct cacctttgac aatcaacttc cctttcgggt tagtcagcag accgcaggtc
    12301 atatcgtatt cgccaggctg taaattcgcc gtcattttct ggctaaaacc aggggcgata
    12361 ttttcccgct cttccaccac catcacgcct ttgaggatct cccactccag cgccttctgg
    12421 ctatggttct gaataatgaa ctgtgttttc ccggcgttaa ccgtaatggt catcggttcg
    12481 cactgtttat ccgtcacggt cactttgact tgcggaatat cagcggcgtt agccataaaa
    12541 gcagaagaga acagcgcagc cacgctcaac tgcaatgcgt tacggcggaa gttaatggtc
    12601 atgacgagac tatccctttt aagtatgttg taactaagca agagtttgcg tcgtatttgc
    12661 tacgcggagc gagacgctgt cgcccctgca cgtggtggca gagcaaacgc caccagcgcc
    12721 gggatgagat aaataaacca gacggcgact tcgctgacgc tcggcgcttc ctgataacca
    12781 aaaatccctt ccatcagcgt gccaaacagc gagtgagttg agagcacctc gctcatatcg
    12841 aaggcgattt cctgaaagtg gttccacaat ccggcttcat gaaatgcgcg aatggcacct
    12901 gcagccagtc ctgcggcgac gaagagaata aacaggctgg tccatttaaa aaatgcacca
    12961 agattgaggc gaataccgcc ccagtagagc aggaagccga gcaccacggc agtagcaaga
    13021 ccgagcattg cacccagcgg cggccagatc ccgacatctt gttgaaatgc cgccagcagg
    13081 aaaaagatcg actccaggcc ttcccttgca acggcaaaaa agaccatcat caccagcgcc
    13141 cagccatgat gatttccacg ctgcaatgcg ctatcgactg cctgctccag ttgcactttg
    13201 acgttgcgcg acactttgcg catccagaaa accatccagg taaggatcac cacggcgatc
    13261 accgccacga taccttcaaa cagttcctgt tctttttgcg gaaattcgcc ggtggtttcg
    13321 ttaatgaaga tacccaagcc caggcacaac gcagcggcaa gcaacacgcc aatccacatc
    13381 acaccaatcc atcggcctcg ctgggtacgc ttaagatagc tggcaatcaa actgacaatc
    13441 agcgcggctt caagcccttc gcgcaacata atgagaaatg gaacaaacat gccacccacc
    13501 cttaaacgtt tttctgagtc aatctatgca aagaaaagta aatcgcagtg atagcgatta
    13561 tcattacgct aaagaaaaat acaagcggaa tgtttataaa atgatgttgg aatgtaaaaa
    13621 tgaggagatc aaatccggag aaaaaagcga ttattttaag gtatttagct gacgtttcgc
    13681 ccggcagatg aagcttgtac gaatgttggt ggcgacaagc tcgctgcagc gtgccccgaa
    13741 gggttgaatt ttcagttagt cgtttcacaa aagaacgttg ttttttgctt attaatagat
    13801 agtgataagg ttgtgaatga aattatacct catctcctca tcgaattttt tgcccacaac
    13861 ctaaacagga gaaaacgtgt atttaagtct ggaatgagcg atttttgata gtgcgtaatt
    13921 aaccc
//
LOCUS       DAZFFG010000121        13288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFFG010000121 DAZFFG010000000
VERSION     DAZFFG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.121"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(681..1772)
                     /gene="ychF"
                     /locus_tag="ACHJ7J_004014"
     CDS             complement(681..1772)
                     /gene="ychF"
                     /locus_tag="ACHJ7J_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO5002185.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1889..2473)
                     /gene="pth"
                     /locus_tag="ACHJ7J_004015"
     CDS             complement(1889..2473)
                     /gene="pth"
                     /locus_tag="ACHJ7J_004015"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO5002186.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2751..3029
                     /gene="ychH"
                     /locus_tag="ACHJ7J_004016"
     CDS             2751..3029
                     /gene="ychH"
                     /locus_tag="ACHJ7J_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO5002187.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3084..4763)
                     /gene="dauA"
                     /locus_tag="ACHJ7J_004017"
     CDS             complement(3084..4763)
                     /gene="dauA"
                     /locus_tag="ACHJ7J_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO5002188.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYILADGSQGNGIPQLLPQLVLPWDLPDSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4888..5835)
                     /gene="prs"
                     /locus_tag="ACHJ7J_004018"
     CDS             complement(4888..5835)
                     /gene="prs"
                     /locus_tag="ACHJ7J_004018"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO5002189.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5986..6837)
                     /gene="ispE"
                     /locus_tag="ACHJ7J_004019"
     CDS             complement(5986..6837)
                     /gene="ispE"
                     /locus_tag="ACHJ7J_004019"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO5002190.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6837..7460)
                     /gene="lolB"
                     /locus_tag="ACHJ7J_004020"
     CDS             complement(6837..7460)
                     /gene="lolB"
                     /locus_tag="ACHJ7J_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO5002191.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7674..8930
                     /gene="hemA"
                     /locus_tag="ACHJ7J_004021"
     CDS             7674..8930
                     /gene="hemA"
                     /locus_tag="ACHJ7J_004021"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO5002192.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLHEADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8972..10054
                     /gene="prfA"
                     /locus_tag="ACHJ7J_004022"
     CDS             8972..10054
                     /gene="prfA"
                     /locus_tag="ACHJ7J_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO5002193.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10054..10887
                     /gene="prmC"
                     /locus_tag="ACHJ7J_004023"
     CDS             10054..10887
                     /gene="prmC"
                     /locus_tag="ACHJ7J_004023"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5002194.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10884..11276
                     /gene="sirB2"
                     /locus_tag="ACHJ7J_004024"
     CDS             10884..11276
                     /gene="sirB2"
                     /locus_tag="ACHJ7J_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO5002195.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11280..12089
                     /gene="sirB1"
                     /locus_tag="ACHJ7J_004025"
     CDS             11280..12089
                     /gene="sirB1"
                     /locus_tag="ACHJ7J_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO5002196.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12125..12979
                     /gene="kdsA"
                     /locus_tag="ACHJ7J_004026"
     CDS             12125..12979
                     /gene="kdsA"
                     /locus_tag="ACHJ7J_004026"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO5002197.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13127..13234)
                     /gene="ldrA"
                     /locus_tag="ACHJ7J_004027"
     CDS             complement(13127..13234)
                     /gene="ldrA"
                     /locus_tag="ACHJ7J_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5002198.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3329 a         3335 c         3417 g         3207 t
ORIGIN      
        1 ttaatatata tttaacatcc cccttacaaa ggtgagttta tatcgagtta ttccctaagt
       61 tgtcgttact tattccaatt cgttactatg aatatgacaa aaatattaca acaaacagcg
      121 gttaaaaaat ttggtgaaga tttttggtaa taatcctaat tagttatggg attttcgctt
      181 gatgtatctg cataacaaaa acaatatgcg ccatttttgc attactaata agaaaaagca
      241 aaaaacatcg atattcattt ttggtcaatg ggttttctgg cctatcgttg ttattgtgct
      301 actggttatc attttcatct tgccaggatc gcacgtaatc acgttattcg gggactttat
      361 ctgcgggata ttacccttca ggtaagtcct tttctcgcca gcatattgga gcgtgatcaa
      421 tattgatcgg ctgtgaaggg ggctgatgtg tagtggaaca aaaactgacg ttatatcact
      481 gctaatgaga tatccgtcat ctgaacactt atgtctttaa ttattctcgt ggttcactgt
      541 aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg tgaaatgaaa
      601 aatccacgca attgcgtgga ttttatatac ttttgcgctc ttcatgagat ttagcgaaac
      661 ctcatgagac aacaaattaa ttagacgttg aaaaggaagt tcatcacatc gccatcttta
      721 acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc ttgttcacct
      781 ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc tttttcaaaa
      841 tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat ggtccatgca
      901 cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt ataaccggca
      961 cggatcacac ggttcaggcc cggctcttca agcccaagct cctgcataaa ctcgtcacgt
     1021 tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg aaccacaaca
     1081 gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt ttcaaaaccg
     1141 tcttcgttga cgttggcgat gtacattgtt ggttttagcg tcaggaagct caggtaacga
     1201 atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc gttttccagc
     1261 tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc gccacctttg
     1321 gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt
     1381 tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac gtgaatgatg
     1441 ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg gatgttggtc
     1501 aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg
     1561 acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc cagttgatcc
     1621 aggcgaggat caggcattgg tacgacgcct gtgttcggct caatggtgca gaatggaaag
     1681 ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt cccgacgttg
     1741 ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat atcttaataa
     1801 tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat tatacacggc
     1861 actcgacaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc aatcggttcg
     1921 ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct tcgtcgatgg
     1981 cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa ccgacaactt
     2041 tatttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta gggttattac
     2101 ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg cccaacttaa
     2161 atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt tcgtccggat
     2221 taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc ataaatgtag
     2281 tcgggactaa caggcggaca tcttcgcctc caagggtgac tcgcgaagta taaccaaaga
     2341 atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca acgaaccagg
     2401 caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg ccgacaatca
     2461 atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta gtttactggt
     2521 tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata attgcctgag
     2581 tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt tgaaataacg
     2641 gttgtaattg tgatcacgcc cgcacataac ccacagggtg ttgtctatac tttacacata
     2701 aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat atgaaacgca
     2761 aaaacgcttc gttactcggt aacgtgctca tggggttggg tctggtggta atggtggtcg
     2821 gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag tatttcgcac
     2881 atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt gcccgtgttg
     2941 gcgggcatga acaggtgtgc gaccgttact ggtgggttcg ccactatgac aaacgttgcc
     3001 gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac cagtcagttt
     3061 gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg attcgggaag
     3121 aacgcgaggc gtcccgggat cggttgaatg cctgcgcgag ccatagtgcg cagtggctgg
     3181 aattccacgt tgcacacgcg cagttcacat ccttcgggta gacgcttcac aaaacgctgg
     3241 aacgcatcaa gaccaccagc atcaagtacc ggaacggcat cccacttcag aatcacaatc
     3301 cgtttgcctt caagacgtga ctccaggtcc gtgaacaagc cttcagcagc agcaaaaaac
     3361 agcgggccaa taacgcgcag aaccaggaca tcgtctggaa catctacgac taccggtgcc
     3421 aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac gatccccacg
     3481 ctgatggcaa taaccatatc aaacagcacg gtcagcgaca tgcacagcag catgacaatg
     3541 atgtcatctt tcggtgcatg acgcagcaag tcgaccactt tatgcgcttc actcatgttc
     3601 cacgccacca tcaacagcag ggctgccata gcggaaagcg gcagccagga gagcagcggt
     3661 gccagtacca gcagggcaag aataaccaga atagagtgga tcaccgccga gataggggaa
     3721 gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagcggtagc ggtaatacca
     3781 ccaaagaacg gagcgatgat attccccagt ccctgtccaa ccagttcgct attcgccttg
     3841 tgtttcgtcc cggtcatacc atccagcacc acggcgcaga gcagagattc gattgcgccg
     3901 agcattgcca ttgagaatgc cgcaggcagc agtgtgcgaa tagaatccca ggttagcgtg
     3961 aattctgaat caggcagatc ccacggcagc accagttgcg gcagcagttg cggaataccg
     4021 ttaccctgag aaccatcggc caggatgtag tggaattgcg aaccgatggt agcaacatgt
     4081 ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc cggaaggtga
     4141 ccgggtaaac gaatgcccag acgcggccag aaaacaagaa tacctagcgt cacaatgcca
     4201 atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc gactttttgt
     4261 agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat ctgcatggta
     4321 ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg aatatactca
     4381 atcaggcgac caaagcgtgc cagacccatc agaatcaaaa agatccccga cagcaaggtc
     4441 gcaaccagca gtcctgccag cccaaactgt tgcgaaacgg gatagagaat taccacaaat
     4501 gccgcagtcg gaccggaaac gctaaagcgt gacccacccg tcagagcaat gacaatcccc
     4561 gcaacagctg cggtatataa accgtactgg ggtgccacac cactaccaat agccaacgcc
     4621 atcgccagcg ggatagcaat aatcccgact gttatcccgg caatcaggtc acgggtaaac
     4681 cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa aggcatcaca
     4741 tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca tctgtcaact
     4801 aaatggcagg tgaaggaggc ataggtcata caaatggata ttaaagacaa aaaaacccgc
     4861 cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga tcgattcttc
     4921 gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag tacgcacgtt
     4981 cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga cttcatcaat
     5041 tacagagtta cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc
     5101 aaatacacgt ttagcaccac gttctttcag agcttcagca gctttacaca gcgtaccgcc
     5161 agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac cgatgatatg
     5221 catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca tatcggtatc
     5281 gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac
     5341 aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc taccaaatac
     5401 gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat ccactgtcag
     5461 cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag tgattggtac
     5521 acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga taacagcggt
     5581 gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt ccatcaggtt
     5641 gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc gtacattttc
     5701 attaatttgt acgctgactt cgccatcgct aaagcgacct acagcggcgt cgccgagtga
     5761 agtgtacagg cggttggcaa tacgttgtgc tagttccggg gtggcgttac cagcaaaaag
     5821 cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa gaatctgccg
     5881 aaaactgtgc gggccaggca tgatcctttc caggcggtgt attaaagagc gcgatgcaac
     5941 gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct
     6001 gtgcaatggg gaaagattag cacctttcgc cacaaagcca ttgagccatt ccggggcttg
     6061 ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac aggcccctgt
     6121 cccagtcagg cgcgacgggg cgtattctaa cagccaggaa agcaccgcat caacctcgcg
     6181 aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta gcaacgtttc
     6241 tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca ccggagtcgg
     6301 aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca ccggggttag
     6361 tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag gaacatctgc
     6421 gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt gccagagatg
     6481 atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac cgccgcccat
     6541 cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc gtcggaagac gcccgctgtc
     6601 tgccgcagtt ttcatcaaca accgcgctgc gcgaacgatc aggttatctt catgttccac
     6661 gccttcaacg ggcgttaaca gacggatatc cccatcgtca cgaagctcaa tgctgatggt
     6721 gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg
     6781 accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg tccgcattat
     6841 ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt gagttccata
     6901 ttggctggca tcgcaggttg cgttttggtg tcataaccac cataaacaac cttccagttt
     6961 ttgccattct ggctgtaggt aatttcgctc aggcggtact ggtcgtccag tttgtagtcg
     7021 gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg aattggcatt
     7081 ccagtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg acctttattg
     7141 tcgactaact gcacgttacc cggttgagca ttcagctcca gttccgtgct gcccaatggg
     7201 ttagtgagca gcagacggta gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac
     7261 actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tctgatactg attaagattg
     7321 cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttgcc aggacctttg
     7381 ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag gcggataaga
     7441 cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca gttataaccc
     7501 ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc
     7561 taacggcttt cggcaattac tccaaaaggg ggcgctctct tttattgatc ttacgcatcc
     7621 tgtatgatgc aagcagacta accctatcaa cgttggtatt atttcccgca gacatgaccc
     7681 ttttagcact cggtatcaac cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt
     7741 tttcgccgga taagctcgat caggcgcttg acagcctgct tgcgcagccg atggtgcagg
     7801 gcggcgtggt gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt gaagagcagg
     7861 ataacctgca agaggcgtta atccgctggc tttgcgatta tcacaatctt aatgaagaag
     7921 atctgcgtaa aagcctctac tggcatcagg ataacgacgc ggttagccat ttaatgcgtg
     7981 ttgccagcgg cctggattca ctggttcttg gggagccgca gatcctcggt caggttaaaa
     8041 aagcgtttgc cgattcgcaa aaaggccata tgaaggccag cgaactggaa cgcatgttcc
     8101 agaaatcttt ctctgtagcg aaacgcgttc gcactgaaac agatatcggt gccagcgctg
     8161 tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg
     8221 tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg
     8281 aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc caaattctgg
     8341 cagatgaagt cggcgcggaa gtgattgccc tgagtgatat cgacgaacgt ctgcacgaag
     8401 ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa ggcatggtgg
     8461 agcgcgcatt aaaaagccgt cgcaaccaac caatgctgtt ggtggatatt gccgttccgc
     8521 gcgatgttga gccggaagtt ggcaaactgg cgaatgctta tctttatagc gttgatgatc
     8581 tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa
     8641 ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa agcgccagcg
     8701 aaaccattcg cgagtatcgc agccaggcag agcaagttcg cgatgagtta accgccaaag
     8761 cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat ctggcatgga
     8821 agctgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg
     8881 gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag cagtacatca
     8941 ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg ccaaactgga
     9001 agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc
     9061 cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg atgtttcgcg
     9121 ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga tgatgctcga
     9181 cgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga
     9241 gcaactggaa cagcaattac aggttctatt actaccaaaa gatcctgatg acgaacgtaa
     9301 cgccttcctc gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt tcgcaggcga
     9361 tctgttccgt atgtacagcc gttacgccga agcccgccgc tggcgggtag aaatcatgag
     9421 cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta gcggtgatgg
     9481 tgtgtatggt cgtctgaaat ttgaatctgg cggtcatcgc gtgcagcgtg ttcctgctac
     9541 ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc
     9601 tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt tccgctcgtc
     9661 aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta ctcacttgcc
     9721 gaccgggatt gttgttgaat gtcaggacga acgttcacaa cataaaaaca aagctaaagc
     9781 actttctgta ctcggtgctc gcatccacgc tgctgaaatg gcaaaacggc aacaggccga
     9841 agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta accgtactta
     9901 caacttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct accgcctgga
     9961 tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg aacatcaggc
    10021 cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg gttacgtgaa
    10081 gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat cctgctggag
    10141 catgttaccg gcagagggcg tacttttatt ctcgcctttg gtgaaacgca gctgactgac
    10201 gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga acccattgct
    10261 catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc agcgacctta
    10321 attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa
    10381 ccttgccgta ttcttgatct cgggacgggt accggggcga ttgcgcttgc gctggctcgc
    10441 gagcgcccgg actgcgaaat taccgctgta gatcgtatgc ccgatgctgt cgccctggca
    10501 caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag cgactggttt
    10561 agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta tattgacgag
    10621 caggaccctc atcttcaaca aggcgatgtc cgctttgagc cgctcactgc gctggttgcg
    10681 gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta
    10741 tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt gcgacaggca
    10801 tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga taacgagcgc
    10861 gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt gttcatctta
    10921 ttagtatcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt tatcagaagc
    10981 atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat acggtgttat
    11041 tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttct gggcaggcac
    11101 agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt tttattgcac
    11161 ttgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg ctggcgttgg
    11221 tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg gggtaagtca
    11281 tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc atgatcctgg
    11341 cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa ctggagcgtc
    11401 tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag cagctggaaa
    11461 aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc gtttatcgtc
    11521 tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt gcggtatcat
    11581 taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg ccggtgattt
    11641 tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg ctgattaatc
    11701 cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag ggaaatatca
    11761 gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt gaggtaatcc
    11821 gcaaattact ggacacactc aaagcctcgt tgatggaaga aaatcagatg gagctggcgt
    11881 tacgcaccag cgaagcttta ttacaattca accctgaaga tccctatgaa attcgcgatc
    11941 gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat ttaagttatt
    12001 tcgttgaaca gtgtccggaa gacccgatca gcgaaatgat ccgtgcgcaa ataaataaca
    12061 tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag attaaggcga
    12121 tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg acctgccgtt
    12181 cgtactgttt ggcggtatga acgtgctgga atctcgcgat ctggcgatgc gcatttgcga
    12241 gcactacgta accgtgaccc agaaactggg tatcccttac gtgttcaaag cctcttttga
    12301 caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag aagggatgaa
    12361 aatcttccag gagttgaagc agacttttgg cgtgaaaatt atcaccgacg ttcacgaacc
    12421 aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg cgtttcttgc
    12481 tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta acgtcaagaa
    12541 accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca aagaaggcgg
    12601 caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca acctggttgt
    12661 cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg tgattttcga
    12721 cgtgacccac gcattgcaat gccgcgatcc ttttggcgca gcttccggcg gtcgtcgtgc
    12781 tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc tgtttattga
    12841 agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc cgctggctaa
    12901 actggaaccg ttcctcaagc agatgaaagc gattgatgat ctggtgaaag gtttcgaaga
    12961 actggatacc agcaagtaat cttttttgct tgaaaaataa agtcttagcg ttctgcgtta
    13021 agactttttt catgggtgcc ggatacaaaa aaggccgcag gctgttaccc tgcggccggt
    13081 ttcgggcgca tattgccatc acggcagcct gacgcccgtt ttcgccttac ttccggttac
    13141 gccaccagcc gacaatcgct gcggtaataa ttcccgccag gatcggtgct gccaggtcgt
    13201 gccagaaaat catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca
    13261 ttttgcggct attcttgcat tgttgatg
//
LOCUS       DAZFFG010000122        13257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFFG010000122 DAZFFG010000000
VERSION     DAZFFG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.122"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..216)
                     /locus_tag="ACHJ7J_004028"
     CDS             complement(<1..216)
                     /locus_tag="ACHJ7J_004028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002199.1"
                     /translation="MPEQVITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEI"
     gene            153..1652
                     /gene="pitB"
                     /locus_tag="ACHJ7J_004029"
     CDS             153..1652
                     /gene="pitB"
                     /locus_tag="ACHJ7J_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO5002200.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1700..2392)
                     /gene="yghT"
                     /locus_tag="ACHJ7J_004030"
     CDS             complement(1700..2392)
                     /gene="yghT"
                     /locus_tag="ACHJ7J_004030"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5002201.1"
                     /translation="MQSIIPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGSRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2566..3279
                     /gene="yghS"
                     /locus_tag="ACHJ7J_004031"
     CDS             2566..3279
                     /gene="yghS"
                     /locus_tag="ACHJ7J_004031"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5002202.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCRSQQGHLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3311..4069
                     /locus_tag="ACHJ7J_004032"
     CDS             3311..4069
                     /locus_tag="ACHJ7J_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002203.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKISVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4115..5464
                     /locus_tag="ACHJ7J_004033"
     CDS             4115..5464
                     /locus_tag="ACHJ7J_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO5002204.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5464..6288
                     /locus_tag="ACHJ7J_004034"
     CDS             5464..6288
                     /locus_tag="ACHJ7J_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002205.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHALKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPL"
     gene            6300..6860
                     /locus_tag="ACHJ7J_004035"
     CDS             6300..6860
                     /locus_tag="ACHJ7J_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5002206.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDQVIALAQKLINE"
     gene            6891..7961
                     /gene="lptF"
                     /locus_tag="ACHJ7J_004036"
     CDS             6891..7961
                     /gene="lptF"
                     /locus_tag="ACHJ7J_004036"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5002207.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7958..9037
                     /gene="lptG"
                     /locus_tag="ACHJ7J_004037"
     CDS             7958..9037
                     /gene="lptG"
                     /locus_tag="ACHJ7J_004037"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5002208.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKMWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQH"
     gene            complement(9072..10244)
                     /locus_tag="ACHJ7J_004038"
     CDS             complement(9072..10244)
                     /locus_tag="ACHJ7J_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5002209.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10244..10492)
                     /locus_tag="ACHJ7J_004039"
     CDS             complement(10244..10492)
                     /locus_tag="ACHJ7J_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5002210.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10524..11438)
                     /locus_tag="ACHJ7J_004040"
     CDS             complement(10524..11438)
                     /locus_tag="ACHJ7J_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5002211.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKYVAEQRLTAMADKITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLMPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11435..13123)
                     /locus_tag="ACHJ7J_004041"
     CDS             complement(11435..13123)
                     /locus_tag="ACHJ7J_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO5002212.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEASAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHDNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         3199 a         3272 c         3436 g         3350 t
ORIGIN      
        1 tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata acactctttt
       61 tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga taatgcgtcc
      121 gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt ggccttgata
      181 tatacacagg gcttttgtta ttacttgctc tggcatttgt gttgttctac gaagcaatca
      241 atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt gccatgcaac
      301 cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta ttgggcggac
      361 ttagcgttgc ctatgccatt gtccatatgt taccaaccga tttgttgctg aatatggggt
      421 caacccacgg tctggcgatg gtcttttcca tgctgctagc ggcgattatc tggaacctgg
      481 gaacgtggtt ctttggttta ccggcctcca gttcgcatac cttgattggc gcgattatcg
      541 gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg ttaaacctgc
      601 gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc ctggtcattg
      661 cgggaggcct gatattcctg ctgcgacgct actggagcgg aacgaaaaag cgtgaccgta
      721 ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaagccg ccattctgga
      781 cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcacacggc gctaacgacg
      841 ggcagaaagg gatcggcctg gtgatgctgg tactggtggg gattgcccct gctggcttcg
      901 tcgtcaatat gaacgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc gtcaccaact
      961 tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattacg atggaacctc
     1021 cattgcctgc ggcatctact gatggcgcgc aagtgacgga gtttcactgt catccggcaa
     1081 atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg
     1141 agccgttaag cgtgagtcag cgcagccagt tgcgccgcat tatgctgtgc atctctgata
     1201 cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac ctgctgaaaa
     1261 aacttcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc atcatggcgg
     1321 tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg atgaccatcg
     1381 gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca caaatgacgg
     1441 cggcagtgtc tatcggcctt gccagttata ttgggatgcc cgtctctaca acacacgtac
     1501 tctcatctgc cgtagcaggg acgatggtgg tggacggcgg tgggttacag cgtaaaacgg
     1561 taaccagcat cctgatggca tgggtgttta ctttaccggc ggcaattttt ctttctggtg
     1621 ggctgtactg gatagcattg cagttgattt aaccttcctg aaatgcccgg tcctggcgat
     1681 cgggcatttc catttttgac tagtgataac cacgcgcggt cataaaatcc gtaatcgctt
     1741 tttctgcatc aaccagcacc tgttccagtg gctgattggc atcgatatca accagttgtg
     1801 caccaccaaa ggttaactgt ggcgttatgg caatcttcct cgccagcgat tcccgtttat
     1861 ggtcgggttt acgcgcgcag gcaacttcaa ggtcaacatt gagtttgatg accagatcag
     1921 gcttatggct cgccatccag tgaaacgctt tacgttcctg gcttgccagc catgagacaa
     1981 aacgactacc ttcaacgtta ggtgggaaca ccgtaccatc gtaagcgccc ggaatttggt
     2041 cctgaggata acggtcggtt agaacaatta acccgcgacg acgacaggca agcatatgac
     2101 gaaagcgcag taagcgacgg gcgacaaacg ctgtaattac cagcgccgga actggtcctg
     2161 gcaatttttt tgctgttttc acctgatttc gttcaattgt tttatgtaag gattttccca
     2221 tcaacggtaa ttttgtcact gcacgaccga catttccggc ctgttttcct aaatgaactc
     2281 tttcggcagc accatatttt tcgacaacgg taatgagatg ttcacacacc gttgacttgc
     2341 ctgaaccatc gctaccaata acggcaatta atggaggtat tattgactgc atgaatatat
     2401 ccttaaattt aaatatccat ccaaaatatt tatttggtta atatgttttt atgaaagcgt
     2461 aattcaggtc aatgtcacaa ttaaccattg tcacaataag gttgaacgga tattctgagc
     2521 acggaccacg taatattcaa tacattattt actgccgggt ttttcgtgaa tcaacgtaat
     2581 atgtcaataa ttaattccac gccagtgcgt gttattgcca ttgtaggatg tgatggttca
     2641 ggtaaatcga ccctcacggc aagcctggta aatgaactgg cagcaagaat gccaacagaa
     2701 cgcatttatc tcgggcaatc gtccgggcga attggcgaat ggatttcaca gctccctgtt
     2761 attggcgcac cttttgggcg ttatctgcga agtaaagcgg cacatgtgca cgaaaagccc
     2821 tcaacaccgc ctggcaatat tactgcgctg gttatctatc tgctttcctg ctggcgggcg
     2881 tacaagtttc gcaaaatgtt gtgtagaagc cagcaaggcc atctgctcat caccgaccgc
     2941 tacccgcagg ttgaagtgcc gggttttcgc tttgatggcc cgcaattggc aaaaacaacg
     3001 ggcggtaacg gttggataaa aatgttaagg cagcgcgagt tgaagctgta ccaatggatg
     3061 gcgtcttatt tacccgtatt gttgattcgt cttggcattg atgaacaaac cgcgtttgcg
     3121 cgtaaacctg accaccaact ggcagcgtta caggaaaaaa tcgccgttac gccgcaactg
     3181 acattcaatg gcgcaaagat ccttgaactg gatggtcggc aaccagccga tgaaattatg
     3241 caagcgtcac tacgcgcaat tcacgccgcg ctttcctgat caccaaaatg gataggggat
     3301 gatttaacga atggatgcac tacaaacaca aactgtaaat agtacaaccg cgccgcagcc
     3361 caactacatt ccggggctga ttgcggtggt cgggtgtgat ggcaccggta aatccacact
     3421 gaccaccgac ctggtgaaat cgctgcaaca acactggcaa accgagcggc gctatctggg
     3481 gctgctctcc ggcgaagacg gcgacaaaat caaacgattg ccgttggttg gcgtctggct
     3541 ggaacggcga ctggcggcca aatcctcgaa aacccaaagc atgaaaacca aatctccggc
     3601 gctatgggcg gcggtgatta tgtactgctt ctcgctgcga agaatggcga atctacgcaa
     3661 ggttcagcga ctggcgcaaa gtggcgttct ggtggtcagc gatcgcttcc cgcaggctga
     3721 aatttcgggc ttttattatg acggaccggg gattggcgtc gaacgtgcga ccgggaaaat
     3781 cagcatgttt ctggcgcagc gcgaacggcg tttataccaa caaatggcgc aatatcgccc
     3841 ggaattaatt attcgcctgg gcattgatat tgagactgcc atctcccgca agcctgacca
     3901 tgactatgcc gagctgcagg acaaaatcag tgttatgtcg acgattggct ataacggcac
     3961 aaaaattctt gagattgatt ccagagcgcc ctacagcgaa gtgctggaac aggcacagaa
     4021 ggcggtttct ctggttgcca tcgtttctga ccgccgaagt ttaacttaag gctggcggga
     4081 tactttttac tatcaacgat aaggtcaggc tgaattggcg ggttttaaca tcaaacattg
     4141 gtttgcagat ggcgcgtttc gcaccattat tcgcaatagc gcctggttag gctccagtaa
     4201 tgtcgtgagc gctttgctgg ggctgttggc gctctcgtgt gccggtaaag ggatgacgcc
     4261 cgccatgttt ggcgtactgg tgattgtgca atcgtacgcc aagtcgatca gcgattttat
     4321 taagtttcag acatggcagc tggtggttca gtacggaaca ccagcattaa ccaacaataa
     4381 tccgcagcaa tttcgcaatg tcgtctcatt ttccttctcg ctggatatcg tcagcggcgc
     4441 ggtggcgatt gtcggcggca ttgccttact gccattcctt tcccattcat taggtctgga
     4501 tgaccaaagc ttttggctgg cggcgctgta ttgcacgctc attccttcaa tggcttcctc
     4561 cacgccgacc ggcattctgc gtgcggtaga tcgctttgat ttgattgctg tacagcaggc
     4621 gacgaaacct tttctgcgcg cagcggggag cgtcgtagcc tggtattttg actttggttt
     4681 tgcgggtttt gttattgcct ggtacgtgtc gaatctggtt ggcggcacca tgtactggtg
     4741 gtttgccgcg cgcgaattac gccgcagaaa tatccataac gccttcaaat tgaatctgtt
     4801 tgagtctgcc cgacacatta aaggcgcgtg gagttttgtc tggtcgacca acattgccca
     4861 ctccatctgg tcggcacgta actcgtgcag cacagtgttg gtggggatcg tgttaggacc
     4921 cgctgccgcc gggttattta aaatcgccat gacattcttc gacgccgccg gaacgccagc
     4981 gggtttactg ggtaaaagtt tttacccaga ggtgatgcgt ttagatccgc gcaccaccag
     5041 accgtggttg ttaggcgtga agtccggttt actggcgggc ggaatcggta ttctggtggc
     5101 gctcgcggtg ttgatcgtcg gcaagccgct catttcgctg gtgtttggcg ttaagtatct
     5161 cgaagcgtat gacctgattc aggtgatgct gggcgcaatt gtgatctcga tgctgggctt
     5221 ccctcaggag tcattgctat tgatggcggg aaaacagcgc gcttttctcg tggcgcaaac
     5281 catcgcctca atcggctaca tcgtattgct gttcatgttc tgtcatctgt ttggcgtgct
     5341 gggagcagcg tttgcttact tcggtgggca atgtctggac gtggcgctct cgttaattcc
     5401 aaccttaaag gcattttttc agcgccattc cttgctttat aacgcagccg gagagaaatc
     5461 ctgatgatga gaaaatattt cccgcttgag gcgagcgagc gtctatttgt tgcgattgaa
     5521 gaagatgatg tcgttgatgc acaggttagt ttgccgccga ctattgcact aagttgtacc
     5581 accgaaatca ttcatgataa ttacgcgtta tgcctgcaat tctggctcaa tggcgttgat
     5641 cgtcaggaat tacttcgttt agttcgcaaa caagccaaag gcgatgagct gactgctgat
     5701 gagcgcaaac aatttaaata catgcgagcg cgctacaagc acctgcgttt tgcgcaacgg
     5761 ctgtatttaa agaagcatca ggccggattt ttattcggca aaacgaccgt ttttctggga
     5821 cgttttcagg acggatttcg taacggtaag aaaaatattg tgtcgtatta cggcaacctg
     5881 ttacgaattt atttaagctc tccggtctgg tcgttggtga actattcatt acgccatagc
     5941 cagttagaaa gcgtaagcgg ttttattgcc tatcggcaaa agcaaatgca tgccctgaaa
     6001 gagataattg ccaaaccgcg actaaccggc agggaatttc atgatgtgcg aaaaatcatc
     6061 agccagcagg tttcttatta cgacacctta agatcgcttg atcctgaaaa taaagaggcg
     6121 ttacagattt cccgtttcct cgccgccatt aatggcctga tgggggataa acacgacgac
     6181 atggtggcgg atgatatgga aaaccgccag tcgtatgatg caccgatggc gctggatagc
     6241 gatattcgcc agcgcttaga gctgctgatt tcgcgttttc cgctataacc ggagcaatga
     6301 tgtcatctcg tttgattatt gcgctgatta tcatgctctt agccccgggt gtgcaggcac
     6361 acaatttcgt cactggcaaa acggttaccc ctgtttatat tcaggaaggc ggtgagctgc
     6421 ttctgaatag cgacgacgaa attcactacc aaaagtggaa cagcacacaa ttggcgggaa
     6481 aagtccgcat tatccagtat atcgccgggc gtaagtcagc gaagaagaag aactcactat
     6541 taattaaagc ggttgaagcg gcaaactttc cgcaggatcg ttttcagccc accaccattg
     6601 tgaacaccga tgatgccatc tttggtactg gctattttgt tgttggcaaa atcgaaaaaa
     6661 ataaacgccg ctatccgtgg gcgcaattcg ttatcgacgg caatggacag ggacgcgttg
     6721 cctggcgctt gccggaacag agttcaacca ttctggtgct gaataaagcc gggcaaattc
     6781 agtgggcgaa ggacggcagc ctgacgccag aagaggttga tcaggtcata gcgttggcgc
     6841 agaaactgat caatgaataa aaaactcaaa ggattgaggg cgttagtgaa atgaaactgg
     6901 ttgaacacta catcatgcgt ggtacgcgcc gtctggtgct gattatcgtc ggttttctga
     6961 tctttatttt cgccagctac tccgcacagc gttatttgac cgaagcggca aatgggacat
     7021 tagctcttga tgttgtgctg gatatcgttt tctacaaagt gctgattgca ctggagatgt
     7081 tgttacctgt tggtctgtat gtgtcagttg gcgtgacgct agggcagatg tataccgact
     7141 cggaaattac cgctatctct gcggcggggg gcagtccggg acgcttgtac aaagctgttc
     7201 tttatctggc gataccgcta agtatttttg tcacccttct gtcgatgtat ggtcgtccgt
     7261 gggcttatgc gcagatttat caactggagc agcagtcgca gtcggagctg gatgttcgcc
     7321 agttgcgggc aaagaaattt aacactaacg ataacggacg aatgatcctt tcgcagacgg
     7381 ttgatcagga taataatcgc ctgactgacg cgctgattta tacttctact gccaatcgaa
     7441 cccgcatttt ccgcgcccgt tcggttgatg tggttgaccc atctcccgag aaaccgaccg
     7501 ttatgttgca caacggcacc gcctatcttc tcgatcatca ggggcgtgac gacaacgaac
     7561 agatctaccg taatctgcaa ttacatctga atccgctgga tcaaagccct aacgtcaaac
     7621 gcaaagcaaa atcggtcacg gagctggcgc gctccgtctt tccggccgat catgccgaac
     7681 tgcaatggcg acaaagccgt ggcctgaccg cattgttgat ggcgctgctg gccatttcat
     7741 taagtcgggt aaaaccgcgg caagggcgat tttcaacgtt attgccactg acgttgctgt
     7801 ttgttgccat tttttatggc ggcgacgtct gccgtacgct ggtggctaac ggcgcgattc
     7861 ccctcattcc tggtttgtgg ttagtgcccg gactcatgct aatgggcctg ctgatgctgg
     7921 tcgcacgcga cttctctttg ctgcagaaat tttcccgatg aatgttttca gtcgctattt
     7981 aatccgtcat ctctttctcg gttttgccgc cgccgcaggg ctattgctgc cgctttttac
     8041 caccttcaac ctgattaacg aactggatga tgtcagcccg ggcggttatc gctggactca
     8101 ggcggtgctg gtggtgctaa tgaccttacc gcgcacactg gtcgaacttt cgccgtttat
     8161 cgccttattg ggagggattg ttggcctggg gcagttatcg aaaaacagtg agcttaccgc
     8221 cattcgcagc acggggtttt ctatcttccg tattgcactg gtggcgctgg ttgccgggat
     8281 attgtggact gtttcgttag gcgcgataga tgagtgggta gcgtcgccat tgcagcaaca
     8341 ggcgctgcaa atcaaatcga ccgccactgc gttgggggag gacgatgata ttaccggcaa
     8401 tatgctttgg gccaggcgcg gtaatgaatt tgtaacggtg aaatcgctga acgagcaggg
     8461 ccagccggtg ggcgtggaga tatttcatta tcgcgacgat ctctcgctcg aatcctacat
     8521 ttatgcacgc agtgccacca ttaaagacga caaaatgtgg atcctgcatg gtgtgaatca
     8581 taaaaaatgg cttaacggta aagaaacgct ggaaacatca gataatcttg cctggcaatc
     8641 ggccttcacc agtatggatc ttgaagagct atcgatgccg gggaatactt tttctgtccg
     8701 tcagcttaat cattacatcc attatttgca ggaaaccggg caacccagca gcgaataccg
     8761 ccttgcactg tgggaaaaac tggggcaacc gatcctgacc ctggcgatga ttttgctggc
     8821 tgtgccgttt acctttagtg ccccgcgctc gccagggatg ggtagccgtc tcgctgtagg
     8881 tgtcatcgtt ggcttactca cttggatcag ctatcaaatc atggtcaatc tgggattgct
     8941 atttgcgtta agcgcacctg ttaccgcgct cggtttaccg atagcgtttg tgctggtggc
     9001 gttgagcctg gtgtattggt atgacagaca acattaaacc cgccgtggcg caacaccacg
     9061 gcgggctcac attactgctt cagagtagca aatgcctgaa tgatggcatc gatttgctca
     9121 cgactgtggg cagcgtttac gctacagcgc agcagcgtga tgcctgcagg cgcggcaggt
     9181 ggcaggataa gattcacata aacgcccagc gaaataagct cgcgccagat gcgtaaaccg
     9241 tcctctttcg aaccgattat taccggcact accgggctaa tatgtgaact taactcataa
     9301 cccagttttt gcagaccgtc atacagatgg ttggcgttat ccatcagctt ttgccgcagc
     9361 tctggttgtg tggcaatagt tcttaacgac gagcgcacgg tcgcaatgca ggacggagaa
     9421 ggagaggcgg taaaaatata cgggcggctg ccgtagcgca gcacttccat cgcttccgaa
     9481 ccgacagcaa aaccgccaat cgaggccaga cttttactga aggtgccaac gataatatcc
     9541 acatcatctt caacgccgac agcttcagcc agtccgcgcc ccgttgcgcc taacacgcca
     9601 aacgaatgcg cttcatcgac aatcaaataa ccaccgagac gacgtttgat atcaacaatt
     9661 tccgccagcg gcgcaacgtc accgagcatg ctgtagatgc cctcaacgat gataatcgcc
     9721 tctttcgccc gttcaccgag gcgaaccatg cgccgttcca gatctttagc atcgttatgg
     9781 cggaaacgaa taatttccgc gccaccgaga gaacaggcgt cataaatact ggcgtggctg
     9841 tcggcatcca gtaacaccac ggcgttatgg tcggcaagtg cgctgatgac gcctaaatta
     9901 gcggtataac cggttgaaaa aacgatagcg gtagggcgat tgaaaaactc ggcaatctct
     9961 ttctctaacg ccaggtgcgg cgcataactg ccgtttgcca tacgagaacc ggtggtgccg
    10021 gtgccttggg ccgctaacgc tgcctgacct tcgctgatgg catcatgatt aaaggttaaa
    10081 ccgagataat tattggtccc ggccagaatc acttttttat tgccgatccg gccttcggtg
    10141 gcggaataca cttcatcaat acaggtgcca aacggtgtta acccgttgtc actaaattgc
    10201 agtcgctcgc cagcaaggcg cgcatattta tcgtatagcc ccattattta ttctccagcc
    10261 atggaagtag agtttccatt aattgcgcag gggttttgac atccagcaga atattaatag
    10321 gaatggaaat atcgaatctg tcttccagca tcattaagag atccatcact ttgatcgact
    10381 ccaggccaag atcgttgacg agatcgctgt cgggcttaat ttccacaccg ttttcgacca
    10441 aatcctgtaa acaggaaagg atgtaatcca ttactatttc acgatttacc atagaaacgt
    10501 taacacacct ttaaacaaga caatcaaaat agcccgttgc gtaatgcgtg ttccaggcta
    10561 atcccgggaa accaattaat atcttcagaa atacggttat tacttgccga ccagtcggcg
    10621 tgggttaatt cacgaatttt cgagcgggtc agcataggtt ctttacctgc caggcgactc
    10681 aacgcggtac tgatatccgc aaggcaggtg agtaccggca gaggaatgcc aaccattcgc
    10741 acggaaccac aacgggcgtc ggcggcaagt tgctgtacgc gttgccagtc atagccgcca
    10801 gcgacgccat cgcataattc ataggtttgc gtctgtacag tttcggcgct taaccactga
    10861 cccactgctt gcgcgaaatc ggtgacgtgc aggaaagaga gctgtgtatc tggcgcacca
    10921 agtcgtggca tcaggccgcg cagcatccag tcaaacagcg gttttaactc tttatcgccg
    10981 ggaccataca cggctgtcgg gcgaaaaacg cccagcgtaa ttttgtcagc cattgcagtc
    11041 agccgttgtt cggcgacgta tttggaattt gcgtaccagg agagttcggg atggcgcgcc
    11101 gccagcgaag agataaacag aaaacgttgg caaaaaccgc tctcttttgc cgcctgcatc
    11161 aggcgcagac tgccgtcaac gttacagcgg gtgaaaatct cttctttgtg cccgcgcact
    11221 tgtccggcgc aatgaaccac cgcgctggct ccggcaacca gttcgctaag cgaatgtgta
    11281 tcttccagcg aaccgcgcac ccaagtaaga ttatcattga cgtgagcgcg ggcagtacgc
    11341 gtcaatgcgc gaacgtgaaa gccgcgggcg agcaggttat cgataatata tttaccgata
    11401 aacccggtag cgcccgtcac cgcgacagtt tgattcatgc cagggattcc tgcacatgaa
    11461 ggcttgcagc ataagccttc tgataacgtt ttttcgcttc cgcacgggca ggcttgccgg
    11521 aggacgttcg gggaatgctg tggggcggca acagatcgat agccgcggtc acgccaaatt
    11581 cgctttggat ccgtgctgcc agcgcgtgga taagctgccc gcgacgttct tcatcgctga
    11641 tccgacactg gatctgcaaa atgatttttt cctgggcggt aacaaaagca atcgcatcgc
    11701 cagaatgaat ttccggttcc tgttccgcta tatattcaat atcctgcggc cagatattac
    11761 ggccacgaat aataatcaga tctttaatgc gtccggtgac atacagataa ccgtccagca
    11821 gataaccgag gtcgccagtg tctaaccagc ccgtcgcggc aatctcgtct tgcgaagcct
    11881 ggtcgccaaa gtaaccgctc atcagactgg gaccggagat gcaaatatgg cctaccacac
    11941 gttccgcgac cggcatacct gcttcattgc ggatttcaat accatgttcc ggcaacgctt
    12001 tgccgcagtt gacgaaagtc gatacggcgc gtgtctctgc acccggcgcg acggctttac
    12061 cctgatattc gaggatgtcg cgatccactt cgttaaccac aaccccggag gcttcatcag
    12121 agaagctgac agccagcgca ttttctgcca gtccgtagca cggcatgaaa gttttatcgt
    12181 caaagttaac ctgacggaaa cattcagcga attgatggag ttgttctgcg gagatgggtt
    12241 ctgcaccaat accagcgacg cgccagcagg aaagatccag ttcagcgaga tctttttcat
    12301 tcacgcggcg ctggcacaat tcatagccaa acggcggcgc aacggaaacg gtgccgcgat
    12361 ttttactgat caatttaagc cattgcagag gacgcatggc aaaatcctga gtgcgcaaat
    12421 aatctactga aagttgcgtg gcgacggggg tcaggagaaa gccgaccagt cccatatcat
    12481 ggtagaaagg cagccaggag acgcagcggt cgccagggcg taatttaatg ccgtcgtggc
    12541 ttatagcacg tagattagcc atcacttcgc gatgggtgat aatgacgcca cggggaaaac
    12601 gggtgctgcc ggaggtgtac tggaggtagg cgatatcgtt cggaactgga cgctggagcg
    12661 caacatcggc ttccggtaat gccttaaacc aggcgtggct taaaacatgt aattcggggt
    12721 tgtcatgcgt cgcggcattg accagtggca accactcatc accagtgata atggctgcgg
    12781 gctggcaact tgccagtaaa ccctgcaatt tggcgctcca ggaatcccgc tgaccaacgc
    12841 ccattggaat cgccaacggg acggcgacta agccggcata ctggcaggca aaaaaagcct
    12901 ctacgaaccc gctactggtt tcggcaatca gtgccacgcg atcgcctttt ttcaggttca
    12961 gcgataacaa ccgcttcgca ctagcttcgg cacgtgcttt taacgtctga tattccagtt
    13021 gatcttcaag ttggcaacgt ctgtcataaa aattcattcc ggcgctactc agagcggcgt
    13081 agtccaaagc atcaaccagc gttggaaaat cggcatagcg cataggtagg gaatgcgtaa
    13141 agattttatt agacatatac accacgaaaa aattggatat caaaaataaa atgacaataa
    13201 tgcggtggat tatataggca taaaagataa atgagatagt attttcatcg gtgtgaa
//
LOCUS       DAZFFG010000123        13216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFFG010000123 DAZFFG010000000
VERSION     DAZFFG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.123"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..569)
                     /locus_tag="ACHJ7J_004042"
     CDS             complement(<1..569)
                     /locus_tag="ACHJ7J_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5002213.1"
                     /translation="MDNAVLNSELIATKAGNITVYNYDGETREYISASTEYLAVGVGI
                     PAYSCLDAPVTSKAGYTICRSVDLKSWEYVSDHRGEIVYNTETGDAKEITALGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDVEAQHGAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQPENTRLNAVLDYIDAVTATDTSTAP"
     gene            complement(569..2032)
                     /locus_tag="ACHJ7J_004043"
     CDS             complement(569..2032)
                     /locus_tag="ACHJ7J_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5002214.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDKALELK
                     VYADDQMAKHLAAPDPHSQYAPKASPTFTGTPKAPTPAAGNNTTQVATTAFVQAALTA
                     LINGAPATLDTLKEIAAAINNDPNFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAGMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVAGLLTYLGLGEGSALPVGVPVPWPSVTPPTGWLKCNGAAFSAEEYPELA
                     KVYPTNKLPDLRGEFIRGWDDGRGIDSGRTLLSAQDGSIEAHGHDYNGVIYTSSGPSW
                     ANTTDAGHRAYSGFTSSYGGSETRPRNIAFNYIVRAA"
     gene            complement(2029..2634)
                     /locus_tag="ACHJ7J_004044"
     CDS             complement(2029..2634)
                     /locus_tag="ACHJ7J_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO5002215.1"
                     /translation="MNSLLPPGSTPLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGETPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            complement(2627..3535)
                     /locus_tag="ACHJ7J_004045"
     CDS             complement(2627..3535)
                     /locus_tag="ACHJ7J_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO5002216.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDKQEAVI
                     RTLELESEPATKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVTRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(3522..3881)
                     /locus_tag="ACHJ7J_004046"
     CDS             complement(3522..3881)
                     /locus_tag="ACHJ7J_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO5002217.1"
                     /translation="MTLYSGMNNTSGKVITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVELTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            complement(3878..4456)
                     /locus_tag="ACHJ7J_004047"
     CDS             complement(3878..4456)
                     /locus_tag="ACHJ7J_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5002218.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGELETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAVYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITMKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            complement(4525..4971)
                     /locus_tag="ACHJ7J_004048"
     CDS             complement(4525..4971)
                     /locus_tag="ACHJ7J_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5002219.1"
                     /translation="MAELQKVDDWLSALLANLEPASRSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTAASTDSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            complement(4964..5395)
                     /locus_tag="ACHJ7J_004049"
     CDS             complement(4964..5395)
                     /locus_tag="ACHJ7J_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5002220.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNAVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAIAEPDAPEEMWTVKRG"
     gene            complement(5358..5606)
                     /gene="lysC"
                     /locus_tag="ACHJ7J_004050"
     CDS             complement(5358..5606)
                     /gene="lysC"
                     /locus_tag="ACHJ7J_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461641.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO5002221.1"
                     /translation="MLTLSGCGNVRHSPEVWLTVSGCPRVTPCRLDQATPRTNGDLNA
                     LLDETEAAWAVCADKVDMIIACQERNSEQTTIPAPRPQ"
     gene            complement(5491..5919)
                     /gene="lysB"
                     /locus_tag="ACHJ7J_004051"
     CDS             complement(5491..5919)
                     /gene="lysB"
                     /locus_tag="ACHJ7J_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO5002222.1"
                     /translation="MNRLLLVVLTLLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSTLLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRKRPALTGGMAYRQWLSASDAVSAGSGNAAH"
     gene            complement(5916..6293)
                     /locus_tag="ACHJ7J_004052"
     CDS             complement(5916..6293)
                     /locus_tag="ACHJ7J_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO5002223.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALAALIKNGPLWQELRASLLKTITRKENVFTSWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            complement(6295..6807)
                     /locus_tag="ACHJ7J_004053"
     CDS             complement(6295..6807)
                     /locus_tag="ACHJ7J_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5002224.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSEEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKIITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDATVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(6788..7003)
                     /locus_tag="ACHJ7J_004054"
     CDS             complement(6788..7003)
                     /locus_tag="ACHJ7J_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1 family phage holin"
                     /protein_id="HFO5002225.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(7007..7210)
                     /locus_tag="ACHJ7J_004055"
     CDS             complement(7007..7210)
                     /locus_tag="ACHJ7J_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO5002226.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(7210..7674)
                     /locus_tag="ACHJ7J_004056"
     CDS             complement(7210..7674)
                     /locus_tag="ACHJ7J_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO5002227.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGAKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(7770..8420)
                     /locus_tag="ACHJ7J_004057"
     CDS             complement(7770..8420)
                     /locus_tag="ACHJ7J_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO5002228.1"
                     /translation="MSLSPARQHRLRVQAEQAASEGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATGFDADLLLQTLELTDGMDM
                     PDQSRARLHKAIGAVLSERNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(8424..9482)
                     /locus_tag="ACHJ7J_004058"
     CDS             complement(8424..9482)
                     /locus_tag="ACHJ7J_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO5002229.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAETGA"
     gene            complement(9499..10332)
                     /locus_tag="ACHJ7J_004059"
     CDS             complement(9499..10332)
                     /locus_tag="ACHJ7J_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO5002230.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEIQEDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQLTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            10475..12241
                     /locus_tag="ACHJ7J_004060"
     CDS             10475..12241
                     /locus_tag="ACHJ7J_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO5002231.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            12241..>13216
                     /locus_tag="ACHJ7J_004061"
     CDS             12241..>13216
                     /locus_tag="ACHJ7J_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5002232.1"
                     /translation="MGKSKKNRAAATNQLKHKSQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQMERWFEPPVSFDGLARTHRAAVHHSSPIAVKRNILTSTFIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRLGGTLSLEPSLAKYTRRGLDLDTYWFVQYGMSTQPYEFTKG
                     SIFHLMEPDLSQEIYGLPEYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNIRHAMKSAKGPGNFRNLFMYSPNGKKDGIQIIPLSEVAAKDEFLNIKNVSR
                     DDMLAVHRVPPQLMGIIPQNTGGFGDIEKASKVFVRNELIPLQERMKEV"
BASE COUNT         2811 a         3911 c         3462 g         3032 t
ORIGIN      
        1 ggcgctgtgc tggtatctgt tgccgtcacc gcgtcaatat aatccagcac agcgttaagt
       61 cgggtgtttt ctggctgcgt cagcttccgc ccggcctgta atttcagttg aatcagactg
      121 atggaagcca ttgctgcatc aatcagtgac tggcgctgtg cttctgccgc ttctactgcg
      181 gcaccgtgtt gtgcctcaac atctgtcacc catttttcac catcccattt atcgtatggc
      241 gttaacgggg cgatagtggt tgtattttcg ggatagtcgc ctagtgctgt gatttctttg
      301 gcatctcccg tttcggtgtt atagacgatt tcaccgcgat ggtctgacac atattcccat
      361 gattttaaat ccacagaacg gcaaattgta taaccagcct tagatgtaac aggagcgtct
      421 aaacaggaat atgctggaat accgacaccc actgcaagat attcagttga tgcagaaata
      481 tattcccgcg tctcaccatc atagttatag acggtaatat tccccgcctt cgtggcaata
      541 agctcgctat ttaatacggc gttatccatt atgcagccct cacgatatag ttaaatgcaa
      601 tatttcgtgg acgggtttcg ctcccgccat atgatgatgt aaatcccgaa tatgcccggt
      661 gtcccgcatc cgtcgtatta gcccaggaag gaccgctaga agtatagatg accccattgt
      721 aatcatggcc gtgtgcctca atgctcccgt cctgagcaga caggagagtc cgtcctgaat
      781 caattcctct tccatcatcc cacccacgaa tgaattcgcc gcgtaaatct ggcaatttat
      841 ttgtcggata aacttttgcc agttccggat actcttcggc agaaaaagct gcaccgttgc
      901 atttcagcca gcctgttggc ggtgtgactg aaggccacgg aacaggtacg ccaacaggca
      961 atgctgagcc ttctcctaaa ccaaggtatg tgagaagacc agctacatcc tttccactca
     1021 aattggtaag cgtattgtcc agcggttgtt tacctgccag cgcattaagc attgtcgtgg
     1081 caaagttcgg atcattcccc agtgccgccg ccagttcgtt cagtgtatcc agtgcagcag
     1141 gtgcagaacc caccattcct gcaatcgccg atttcacaaa agccgtagtg gcaatctgtg
     1201 tattgttgac cgactgcgcc gccgtggggg ctgttggcgt tccggtgagt gccggactcg
     1261 acaacggtgc ttttagtgcc agcgcattgt taatggtggt actgaaattc ggatcattgt
     1321 taatggctgc ggctatttct ttcagcgtgt ccagcgtggc tggcgcacca ttaataaggg
     1381 ccgtcagtgc cgcctgtaca aacgcagtgg tcgcaacctg cgtggtatta ttccccgccg
     1441 ctggcgttgg cgctttgggg gttccggtaa atgtcgggct ggcttttggc gcgtactgtg
     1501 aatgcgggtc cggtgcggca agatgttttg ccatctgatc atccgcgtac accttcagct
     1561 ccagtgcctt gtcatccaca tacttgcggg ttgccagcac tacagcaggg tcgattttca
     1621 gggtgatatt gtccgtgctg ctggtaatca gcaccatgcg cacggtctga gtgcgcccgc
     1681 taccttcagc cagttgcggc ttatagcttt ccgggcagtt gcccacggca atcaatgccc
     1741 cggactcatc aaacaagccc acttcacgta tccaccaacc gccctcgttt tcagggatca
     1801 cctgttcggc aataatctgg ctgctgttct gcgggtcgat ataaagcata ttcagcgcag
     1861 cccggcgttt ctcatttacc agtgccgtct gctttgcgtc cggcgttggc aatactccac
     1921 cgccatcgcc caccgccata tgggtaattt ttagcggcac accgagcgcg gcggcgctgg
     1981 caagtttcgc cgcgccaata tccgtcagca gggtataaaa ttttgtgctc atggattcac
     2041 tctcattgtg tcaataacat ggaccgcccc gccttcatgc gcggtgccac cggaaataat
     2101 cgtttcgttg atatacggat agatcgtgat ttcttcgcca agatagctgg cggctcccac
     2161 ccaatgcggg ccgctggtct gcagattgat ggacatgccg atcatgtggc ggctacatgg
     2221 tttggcatcg cttatcagtc gctcaagttc cagataggta tcttcagtga tgccctggtc
     2281 ctgcacgccg atatccaggc gaaacgtgcc cggtgtttct ccggtctgcc accactcaat
     2341 aatgcggatc aggaatccga acggctccac cacccgccgc acggcactgg tggtcccttt
     2401 atgctgatga atataaaaag catccttcac cacctggcgc ttgacgcttt ctgtccagcc
     2461 ctcgtcccag cgatccacag agaacgccca ggcgagataa ggcaggaaac tgaccggaca
     2521 ggttgccgga ttccacaagt cacgaagcgg cacctgcaga tcagaaatcc cgctgcaggt
     2581 ttgcgccagt cggcgctcca gtggtgttga acccggtggc agcagactat tcatccgttc
     2641 ctccgttggt tacgctccac tgcgtacatg atgccgcctg tgttttgttc aggaccacat
     2701 ccgccagagg agaagccagc tccacacgct gcaccccctc aacatgcagg gcggcaaaga
     2761 tggcgctacg gcgaatatcc cgaccaagac gcgtctgact ggcgatgtac ttctgcaggc
     2821 tggcttttgc cgctgccatt accggctctg cttccggtcc aggatagaga aaaatggtgg
     2881 cttccacgcg atacgggatg atttctgcgc tgcgaaccgt aagacggtca gccaccgggc
     2941 ggacgttctc actgttcaga gctttttcca ccacgtccag caggtctttt tctgcagttc
     3001 catcgccttc gcggctaagg acagtcagca ccacctctgc aggtgccggg ctggttgcac
     3061 tggcatccgc cacccgaccg tcggcgcttc gggcatgaaa ttcataagct gcagttggcc
     3121 ccgcaacaga aagcccttca aaggctgcag gcacacgcag gcgcaacgct tcatcgcttt
     3181 ccatcacagc tgcaacgggc ggcacagcat cattatcagc aggcgtcacc gtcaggcgtg
     3241 tcacgttgta gttggcagcg agctggtcaa gatcgccgcc catcgcgtaa gccaccatca
     3301 ccgcctgcgc ggcttcgtta atgcgctggc gcagaagcaa ctcacggtaa gcgttctcct
     3361 gcaacaattt agtggcgggt tcagattcca gttccagcgt gcggatcact gcttcctgct
     3421 tatctttcgg atgaagcgcc acaaattctg ccttgcgttc ggcaagcagc gtctcaaagt
     3481 ccggcacatc cacaatctgc ggtgcaggca actgcgaaag gtcaatcact gccattctct
     3541 gctcctgttg atacggaaag ggacacaggc acaccgttat tccgccgccc ggtcagctcc
     3601 accaccattg aaccgtcaaa attgctgttg atggtgatgg aatccagcgt caaccgtggc
     3661 tcccagcgac tcagcgccac atacactgcc gacatgacct gcaggcgtaa tgccggattt
     3721 tgtggctgat ctatcagtgc cgacagcagg gaaccatatt cccggcgggc aatacggcta
     3781 ccctgcggtg tcagcagaat gtcccgcacc gactggcgca gatgatcaat atcagtaatg
     3841 actttgccgc tggtattgtt catcccgcta taaagcgtca taccgggcct ccggttgtat
     3901 cgccgccttt caggacgcca gtatgctgat gcgcatcaac cacgatcccg ttagaactca
     3961 tcgctccgcc gccctgggta acgccaccat tgatcaccac ttcgctgtta atgcgcgtgc
     4021 ggtcagcctc cagtacaaac tcactggttt tcatggtgat gttgtcagca gcctcaatga
     4081 ccattgattt gatgcccctg acataccagc gcccggtggc gggttcgtat tcaaaccagc
     4141 caccgtcagg atgttctgtc acgcaggcgt ccgccgacgt cgacggtggt gcgaactgat
     4201 tcgaatagac agcgggcagc gcaaaggcgg tttccagatt gccgcccaga ctcagcagca
     4261 ccacctgctc accttccgat ggtcgccacc atgtacgggc attcccggca cgcagcgtca
     4321 gccagctgat ccagttggtt tcaagctcgc ccgttttcac ccggcaaagc cagttttccc
     4381 tgtccacttc ggtgactacc cctgtgcgga tcaggttggt gataaggcgc atgatttcgg
     4441 ttaattgtgc gttcataggg aaaggttgcc atcaggggaa gaaaggcggc agtgctgcaa
     4501 cttgtatcag tgctgataca aagatcaccc cgccagccat tgcagaatca tgtcgcgggt
     4561 cattgcctca acatcatcat ttacacccag aaggcgacgc tctgcgtaac gcacctccgg
     4621 tcccttacga ctgacgcgat ctcgcaggcc gtaatggtga acgcgggcaa tgcgctgcac
     4681 cttgccttca aactgcacgc tggcagagtc ggtgctggcg gcagttttca ggtattttgt
     4741 ggtgcgcagc tttgcaaaca tctgacgttt gatacggcct tttttactgc gtgctgttac
     4801 ccgtcgcggt tcatagctgc tgccgtcagg gttgcgctgc atcctgatat tctgctgctg
     4861 tgtccggcga agttcctgcg ccagctggcg catcatgcgg cttctcgagg ctggttccag
     4921 attcgccagc aaggcactca gccagtcgtc caccttctgc agttcagcca cgtttcaccg
     4981 tccacatttc ttcaggtgca tcaggttccg ctatagcttc aacgctcgac acactgccgt
     5041 cagtgctgac cagcacacgt tccgtcagtt gcaggttcag gctgatatca cagacatcgt
     5101 tgcgcagaat atccacctca aaggtgaata gcttttcccg taacgccggg ttattgatgg
     5161 catcgggctg gttatccctc agccacagca aaaccggggc catcagcaga ttctggtcgc
     5221 cgctgaaatc ctcaatcacc gcgttcagtg tgtaacggta ctcccacgac atggagctgg
     5281 cccccgtggc aaccagcgaa ccgttatcca caaacagatg cagtttgtcc gggttattgc
     5341 ggacataagg caccgcttta ttgagggcgt ggcgcaggga ttgtggtttg ttcactgttt
     5401 cgctcctgac acgcaataat catgtccact ttgtctgcac agaccgccca ggcggcctcc
     5461 gtttcatcca gcaacgcgtt cagatcaccg ttagtgcgcg gcgttgcctg atccagccga
     5521 cacggcgtca ctcgcggaca accactgacg gtaagccata cctccggtga gtgccggacg
     5581 tttccgcagc cggataatgt cagcaggcaa aggagtatca gcccagcggc gtaaatcctc
     5641 gttctcacgt ttcagttcct cgatccggtg ttgtcgttgt ctcagcagcg tgctggtctg
     5701 ttctgcttcg gcatagagcc gcgcctgctc ccggttattg gtttcagtca gaatggacag
     5761 gctgataagc tggctattgc tctttgccag tgcctggctt ttgctctgca gctcgtctgc
     5821 ctgcgtgctg atggtctggc tggcatcagc cagccgccac gtctgccagc ccagcgccgc
     5881 cagtaataac gtcagcacaa ccagcagcaa ccggttcatg ctgctacctg ttgcgccatc
     5941 tgattacggg tgatccagaa ggcaataacg gtcagtagat aaaagaccag ggtaatagcc
     6001 caccccgtcc aggcgagact tacaacaatc agcaatcgca tcacccaact ggtaaatacg
     6061 ttttcttttc gggtaattgt cttcagcaaa gatgccctta actcctgcca gagcgggcca
     6121 ttcttaatta acgcagccag tgctaccgga attaccgccc atgtcagcaa acaggctacc
     6181 caaacgccgg acgctgccag taccggaaaa atcccctgcg gatacaccat tgctgcgatt
     6241 aacagcgcca tccataacat cagaaacagt ccgctgatta atttcttttt catttcagtt
     6301 tgctccctgt aaacaccagg ccatctcccg cgcacggcgg ttatccagcc cctgattaaa
     6361 aacacctttc acataaaccc agcgcggcaa ctgtcggcat gcatccgccc agcgccgctg
     6421 attgagcaat ttcaccagcg tggaactaca ggcattgccc gtccccacgt tgaaggcaaa
     6481 cgacaccgta gcgtcataca ccttctgcgg cggctgttgc ttcacacacc tttccagcgc
     6541 ccgctccact cgtagcacgt tggagatcag cccttcagct gcctgtcgtt ccgtaataat
     6601 tttgccggga atgacgcccg acgtattacc aatgccgtcg gtccagacac ccgcgctgca
     6661 ctgatacggc tgcagacgac agccttcata atcggcgatc agtttcagtc cctcctcgga
     6721 ggtgtgaagc tgctgaaacc ccggcagcgt ggcagcaata gccagcacgg ccccgacaag
     6781 gcagcgttta acgattgatg gattcatagt cctcccgcga gatctgcccg tcgcgcagaa
     6841 gctggtaggc tttgtgtttg tagtaccagt tgatagccag catcagcaca ccgatcatca
     6901 ggccgcccag cgttgaggca tccttgatgg acaaatcgcc cagccaggcc agcacgacgg
     6961 cgatgcaata cgtgataaag gcgctgattc gctcaagcgt cataattcag tcccatagct
     7021 ggacggtctg cacggtggtg gttgtcggaa tgtccggcag ctccacctgc agcccgtgag
     7081 gtaaaaaggg gccgtattcg gcaagccccg gatttgcctt cagtacctgc tccgtgacac
     7141 cctgcgtgcg cccgtaatga cgccagcaaa gtgcgtccac cgtgtcatac tgatgcgcac
     7201 gcactttcat cagataagct ccactgtgca gtgcggcgca tcctgcaccc ggctgatggc
     7261 ccagcgggcg tcacgccata aatcaccgct tgcttccgcc agttcctcgc ctcgcttcgc
     7321 accggatgcc gtggcgtcat agtcctggta acgttcgttg agcatggcgc gtgcccagca
     7381 ataaaccgcg ttgaaatagt gctgaatgcg ctcgcttttg ccgtccagtt gttccgccgg
     7441 gacttctgcc agcgaggcat accccagcat ctgctggcgt ctgcgaaact catacagctc
     7501 tgcgttgacc tccgaaattg ccgacagggc aacctgtttt aaacgcggct gcgtcaccgt
     7561 gccgtcagtg cgcatcacac tgcgaaactc cgacaggtcc acatcaggcc agaacggcgt
     7621 attcctgatg atttccgcct gttccggtgc ctgttctggc gcaacaaact tcatgctgct
     7681 ttctcctgaa atagagggcg gtggacgggg ttttgatgtg gcagtgcctt tcgccacccc
     7741 gtgccgcccg tgcgcggggg cacgttctgt cagcggctgt cattgcgcag tctgcgctcc
     7801 agctgctgtt tgtctttttt cacgccacag cggggatcga gctgtaacgc atggttgaga
     7861 tgattaaggg cagacgccgg atttctttca ctcaggacag cgccaatcgc tttatgcaga
     7921 cgcgcccgtg actggtccgg catatccata ccgtctgtca gctccagggt ctgcagcaac
     7981 agatcggcat caaagccggt ggcggcaagc attgcgctct gggctgcatc tgccatttcc
     8041 tctgccagca cggtctgcac gttgcggtta cctaacggca tcacccagcc atgacgcagg
     8101 gcgtgacgcc cgatctccag cgccccggca taatctccgg catcaatgcg ccacagcatc
     8161 acgtacatca gcacgtcatc ctgttgagcg cctccggcag ccaggacacc ctccgcccaa
     8221 gcggcgtact tcggcagcag ctccaccttg atttccgctt ttttgaccgt ggactgaacg
     8281 cccttgagac ggcggcggtc ttccgccagt tgcagcagca tcaggtcata gcccgacgcg
     8341 tggcgaacgc tgccgccctc gctggcggcc tgttcagcct gaacgcgcag gcgatgctgc
     8401 cgtgcgggac tcaggctcat gaattacgct ccggtttctg ctgctgcggc gctgaagtcg
     8461 ccaatctgga tgttttccac cagtgcggcg cagcggtagt cctcaaccac ataggcttcg
     8521 ttaacggatt caaagttttc aatccggtca cgtttcgggt tgtcgataac tgagcggcgg
     8581 cgggtgtctt cctgccagta gatggacagg ttatccagac gggtgatcag cagtgcattc
     8641 ggcgggaaga acggcgcacg cacggcctgc aggccaccca tgcgtttctg actgatgatc
     8701 atatcggcag ccagtttttc actgttttcc tgctctttgt tgaccagcgg gaaatacttg
     8761 tcagacagca gttcacgacc gcaaatcacc accagatcgt catcgtcctg gtagaccaca
     8821 tcgataagct cattgacggc atccatcacc acggcgtcca ggttggcata ttcgccacct
     8881 ttcccgactt tcaccgcacc cggtgtggtt tcaccgcctg tggtggtgct gcccatgacg
     8941 tgatccggtg catcttcacg gattttctgc agccagcctt tattcacatc ctgcagtagc
     9001 gggttttcgc tacggttgga ggttttcgca cgcttcacgc cgttaaaacc gatcatgatg
     9061 cggtccagtg cctgacgttt cacgatggca tcacggatgc gcacctggaa atcctgaaac
     9121 ttcgcccaca ggtccagctt cgcgtaggtc agtaccgtgt caaagttggt ctgctcgcat
     9181 ttgtattcca catcgaccat cagcgtcgga tcgacaggct cacgctcttt tgcggtggta
     9241 tcagtggttc cggcaatggt gctgccaaca cccagcccca gcaactgacc agactgctca
     9301 gtcactggcg tgacgttaat cagcgtcagg aatgcggcgg attgctggat ctggtcttcc
     9361 agcgtctgct gcacggacgg ctccacggtg aacttgctgg acagttcttc aactgccaca
     9421 ccgttcagac gcgccagctg ctgcaggtaa gcgttaaaag caaagcgggt attcttcttc
     9481 atcaggtttt gtgctccatc agcaattggt cagagtgtca gcgggggcgt taccgcctgt
     9541 tgcacgctgg cggtagtcct ggcggctgtc ttcatggctc agcttgtcca ccagttcgtt
     9601 aaaggcggtc agctgtgcct gcagggcagt ctccagctca gacaggcgtt cttcctgctc
     9661 agacagggat ttttcggtgc gcgcactcag gttctgctgc tcagtggcga ccagttccac
     9721 ggccttatgc acatcagaga accgggcgtc atcggactgc tcttttttgg tgaacagcgc
     9781 cgtgacacgg gcaaacaggg acggtttgtc ctcctggatt tcttccagtt cgatcaccgt
     9841 ttcctctgca gcggtaaaga gattggcagg attctgcttg cggtttgcca gcgggttatg
     9901 ggctgcactg gcgctgaatg tcagcatttc agtgcccaga ctggcagggt catcagtggc
     9961 agccaggccg accaggtagg ctttgcccgt atcagcgaac ttcgggctga cttccataga
    10021 ggtgaataat ttctggcctt ttttcaccag ctccaccagg gactccgttg gctcaacgtc
    10081 ggcatacagc gccatcttgc ctgccagcgg accttccgtg atttcttcag caaacagcgc
    10141 cgtcaccttg ccgtagcggt taaaggtgct gtccggcaga taagacttga tgtgctcaag
    10201 gttaatcagc gcggtataca ccgccgggtt gtagctggct gccatctgtt ccagccattc
    10261 acgctggatt tcgcgtccat cggtggtggc accttccacc ccgatgcgaa aacgctttgc
    10321 tttcactgtc atgagccgtg ctccgttaga aaaaacttac tggagcctta tggttgcggt
    10381 gatggaggca gtgaaacaat gcgcggtatt tgtaccgaca accacacaaa ccgcaggcgg
    10441 ggaaagcctt cattcaaggc tgtaggtttg tgccatgaac accacactga cacccgcaga
    10501 tctcgatccc cgtcggcagg ccatgctgct gtactttcag ggataccgcg tcgcccgcat
    10561 tgctgaaatg ctgggcgaga aagttgcaac cgttcacagc tggaaaaaac gcgacaagtg
    10621 gggtgactat gggccgctgg atcagatgca gctcaccacc gccgcacgct actgtcagct
    10681 cattatgaag gagcacaaag aagggaaaga tttcaaagag attgacctgc tggcgcgcca
    10741 gtctgagcgc cacgcgcgga tcggcaagtt taacaatggc ggcaacgaag ctgacttaaa
    10801 ccctaacgtc gccaaccgca acaaaggccc gcgccgtcag ccggaaaaga atgtcttcac
    10861 cgatgaacag attgagaagc tggaagaaat cttccattcc tccatgttca actaccagcg
    10921 ccactggtgg gaagccggaa aaaccaaccg catccgcaac ctgctgaagt cacgccagat
    10981 cggcgcgacc ttttactttg cccgtgaagc cctgattgac gccctgctta ccggacgtaa
    11041 ccagattttc ctttccgcca gtaaggctca ggcccacgtc tttaaacaat acatcatcga
    11101 cttcgccaaa gaagtcgagg tggagctaaa aggcgatccg atggtgcttc ctaacggagc
    11161 cacgctttac ttcctcggca ccaatgcccg cacggcccag agttatcacg gcaacctgta
    11221 tctggatgaa tatttctgga taccgaaatt ccaggagctg cgcaaagtgg cttccgggat
    11281 ggctattcac aaaaaatggc gacaaaccta tttttccacg ccatccagcc tgacccacag
    11341 tgcttatccg ttctggtccg gtgcgctgtt caaccgtgga cgcaacaaag ctgacaaggt
    11401 ggacatcgac ctgtcccaca gcaatttggc ccccggcctg ctgtgcgcag acgggcaata
    11461 ccgccagata gtcaccgtgg aagatgcggt gcgcggcggc tgtaacctgt tcgacctcga
    11521 ccagctacgc atggagtaca gcccggacga ataccagaac ctgctgatgt gcgagttcgt
    11581 ggacgatctc gcgtccgtgt tcccgctcag cgaactgcag gcgtgcatgg tggacagctg
    11641 ggaagtctgg accgactttc atgcactggc cctgcgcccg tttggctggc gcgaagtgtg
    11701 gatcggttat gacccggcaa aaggtacgca gaacggcgac agtgccggat gcgtggtggt
    11761 ggctccgcca gccgtgccgg gtggtaagtt ccgcattctt gagcgtcacc agtggcgcgg
    11821 gatggacttc cgcgcccagg ctgacgccat caaaaaactc accgaacagt acaacgtgac
    11881 ctatatcggt atcgactcaa ccggcgttgg tcacggggtt tatgagaacg tgaaagcgtt
    11941 ctttcctgcc gtccgggagt ttgtctacaa ccccaatgtt aaaaacgccc tggtactcaa
    12001 ggcctacgac attatcagcc accgccgtct ggagtttgac gccgggcaca ccgacattgc
    12061 gcagtcattc atggcaatcc gtcgcgccac caccgccagc ggcaaccgcc cgacctatga
    12121 agccagccgc agcgaagaag ccagccacgc cgatctggcc tgggcaacga tgcacgcact
    12181 gtttaacgaa ccgctgcagg gcgaatccgc caataccagc aatattgtgg agattttttg
    12241 atgggaaaga gtaagaaaaa ccgcgctgcg gcgacgaatc agctcaagca taaaagccag
    12301 acttcagccg aagcattcag ctttggcgat cccgttcctg ttctggaccg ccgtgaactg
    12361 ctggactatg tggaatgcgt acagatggag cgttggtttg aaccgccggt gagttttgac
    12421 gggctggcgc gtacccaccg tgcagccgta catcacagct caccgattgc cgttaagcgc
    12481 aacatcctga caagcacgtt tattccgcat ccgctattga gccagcaggc attcagccgc
    12541 tttgtgcagg actatctggt attcggtaat gcctatctgg aaaagcgcac taaccgcctc
    12601 gggggcactc tgtcgctgga gccatcgctg gcgaaatata cccgccgtgg cctggatctt
    12661 gatacctact ggtttgtgca atacggtatg agcactcagc cctacgagtt caccaaaggc
    12721 agcatttttc acctgatgga gccggactta agccaggaga tttacggcct gccggagtac
    12781 ttgtcagcca tcccttccgc cctactgaac gagtccgcca cactgttccg ccgcaagtat
    12841 tacatcaacg gcagccatgc agggttcatc atgtacatga ccgacgccgc acagaaccag
    12901 gaagacgtga acaacatccg ccatgccatg aaaagcgcca agggcccggg caacttccgc
    12961 aacctgttta tgtactcacc taacggcaaa aaggatggca tccagatcat ccctctgtca
    13021 gaagtagcgg caaaggatga gttcctgaat ataaaaaacg tatctcgtga tgacatgctg
    13081 gcagttcatc gtgttccgcc acaattaatg ggcatcattc ctcagaacac aggaggattc
    13141 ggtgacatag aaaaagccag caaggtattt gtgcgtaacg agctaatccc actacaggaa
    13201 agaatgaagg aagtaa
//
LOCUS       DAZFFG010000124        13064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFFG010000124 DAZFFG010000000
VERSION     DAZFFG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.124"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..286)
                     /locus_tag="ACHJ7J_004062"
     CDS             complement(<1..286)
                     /locus_tag="ACHJ7J_004062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002233.1"
                     /translation="MLESLAFVPPVLKTASAVFTSRKMIFSAVCSCVGMAMSSKIVVT
                     GMAGAGKSYLFESIVQESKNKKMKRPGQSVKGEDHILYIGNGLLPKKITII"
     gene            682..1308
                     /locus_tag="ACHJ7J_004063"
     CDS             682..1308
                     /locus_tag="ACHJ7J_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5002234.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKLKGWQIAGEYIENASG
                     AKLERIELMRLLSEAQPGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGVYIG
                     REKNQEAREIVQEMLERGVKPELIMKAAGISRATYYRIKSELSVIKSE"
     gene            1644..1826
                     /locus_tag="ACHJ7J_004064"
     CDS             1644..1826
                     /locus_tag="ACHJ7J_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5002235.1"
                     /translation="MWRDSIFHSAMHSLMNSQSRDTRDRSMNDDNVLEISEPCIRSTF
                     ERLYQNMNEVFGLGDY"
     gene            complement(1906..3153)
                     /locus_tag="ACHJ7J_004065"
     CDS             complement(1906..3153)
                     /locus_tag="ACHJ7J_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO5002236.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADVGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3225..4139)
                     /locus_tag="ACHJ7J_004066"
     CDS             complement(3225..4139)
                     /locus_tag="ACHJ7J_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO5002237.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRNICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDERGMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4355..5788
                     /gene="cscA"
                     /locus_tag="ACHJ7J_004067"
     CDS             4355..5788
                     /gene="cscA"
                     /locus_tag="ACHJ7J_004067"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO5002238.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(5796..6791)
                     /locus_tag="ACHJ7J_004068"
     CDS             complement(5796..6791)
                     /locus_tag="ACHJ7J_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5002239.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            7022..7240
                     /locus_tag="ACHJ7J_004069"
                     /pseudo
     CDS             7022..7240
                     /locus_tag="ACHJ7J_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7258..8586
                     /gene="dsdA"
                     /locus_tag="ACHJ7J_004070"
     CDS             7258..8586
                     /gene="dsdA"
                     /locus_tag="ACHJ7J_004070"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO5002240.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(8694..10232)
                     /gene="emrY"
                     /locus_tag="ACHJ7J_004071"
     CDS             complement(8694..10232)
                     /gene="emrY"
                     /locus_tag="ACHJ7J_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO5002241.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10232..11395)
                     /gene="emrK"
                     /locus_tag="ACHJ7J_004072"
     CDS             complement(10232..11395)
                     /gene="emrK"
                     /locus_tag="ACHJ7J_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO5002242.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            11811..12425
                     /gene="evgA"
                     /locus_tag="ACHJ7J_004073"
     CDS             11811..12425
                     /gene="evgA"
                     /locus_tag="ACHJ7J_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO5002243.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            12430..>13064
                     /locus_tag="ACHJ7J_004074"
     CDS             12430..>13064
                     /locus_tag="ACHJ7J_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5002244.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIG"
BASE COUNT         3815 a         3041 c         2868 g         3340 t
ORIGIN      
        1 gaatgatagt tatttttttt ggcaaaagac cattacctat atataaaata tgatcctcgc
       61 cttttactga ttgccccggt cttttcatct ttttattttt agattcttgc actattgact
      121 caaaaagata agatttaccc gcaccagcca ttccagtaac tactatctta gagctcatcg
      181 ccatcccaac gcaactacac actgcggaga aaatcatctt tcgcgatgtg aaaaccgcac
      241 ttgctgtttt caatacagga ggaacaaaag ctagactttc aagcacaaaa cgtaccttta
      301 tcagtttaga ggtgatactt tagtctaccg aaaaaattaa ccatttaaaa tcagtttgat
      361 aactcgaaaa acaaaatatg cggctcaaac caagttaacc caaatagaat gtatgggtaa
      421 ggattagatt tcaacttaca tgctaggcaa ccccaccagc acaacaaatt atctcatacg
      481 gtaaaaacgg tgtcgggctg tttctttcgg ctagctcaat atatcacctt aaacaccagc
      541 cgttatcatt gtctcaattg ggtataccgt aatgacaagg tgatacaaag tctcaatagg
      601 gttgtatagg aacattttga ctacacaaaa tgccttatgt atcatttgag gttatattca
      661 caaccctatt gagacaatgc aatgaacgtt aggatttact gccgagcatc aactgagggg
      721 caacacgctg atcgagcact taccagccta cgtgaatttt caaaattaaa aggctggcag
      781 attgcaggag agtatataga aaatgctagc ggggcgaagc ttgagcgcat agagcttatg
      841 cggctattgt ctgaagccca gcccggcgat ctgttgctag tggaagctat agatcgtctg
      901 agtcgcctag aacatagcgc atgggttgaa ctgaaagata cgctcaaccg aaaagggtta
      961 atcatcgtat caatggattt gccaacgagc tggcagatgg tcgagatggc aggcaatgac
     1021 ctaaccagcg gcatcctgag agctgtaaat gccatgttga tagatatcct tgccacaatg
     1081 gcgcgacagg attatgaaac acgccgtaaa cgccaacagc aaggcataga gcgggctaaa
     1141 agcgaaggcg tatacattgg cagggagaaa aatcaggaag cccgtgagat cgttcaagag
     1201 atgttggagc gaggagtaaa acctgaactc atcatgaagg cagcaggaat cagccgagcg
     1261 acttactacc gtataaaaag cgagctgtca gtcataaaat cagaataaca tttgaaaggt
     1321 agtcaggctt gtttcatcag ctaaaaccag tttttctgac cacaacatct gggggcaccc
     1381 attggggcac taacaaaaat cgaaaacaaa attaattata attcaaattg ttaatttatt
     1441 tttcgattcc tgcaggggac accagatacc cttcaaacca aatctacctt catcccgtaa
     1501 acgatgggtt tggcagcaca cttgccctat atctactcat ttttactgca acaggttgaa
     1561 atctcagcac tgtcagaaag cgctgatgac taaacagccc tgggccgggc gatgtaacca
     1621 tcacacagaa tcctgatagc gaaatatggc gtgactcgat atttcactcc gcaatgcatt
     1681 ccttgatgaa ttcgcagtcc cgtgatacac gggacaggtc gatgaatgac gacaatgtcc
     1741 tggaaatcag cgaaccgtgt atccggagta catttgagcg actgtaccag aacatgaatg
     1801 aggtgtttgg attaggcgat tattagcagg gctaagcatt ttactattat tattttccgg
     1861 ttgagggata tagagctatc gacaacaacc ggaaaaagtt tacgtctata ttgctgaagg
     1921 tacaggcgtt tccataacta tttgctcgcg ttttttactc aggaagaaaa tgccaaatag
     1981 caacatcagg catacaatac ccgaaattgc gaagaaaact gtctggtagc ctgcgtggtc
     2041 aaagagtatc ccagtcggcg ttgaaagcag cacaatccca agcgaactgg caatttgaaa
     2101 accaatcaga aagatcgtcg acgacaggcg cttatcaaag tttgccacgc tgtatttgaa
     2161 gacggatatg acacaaagtg gaacctcaat ggcatgtaac agcttcacta atgaaataat
     2221 ccaggggtta acgaacagcg cgcaggaaag gatacgcaac gccataatca caacaccgat
     2281 aagtaatgca ttttttggcc ctacccgatt cacaaagaac ggaataatcg ccatgcacag
     2341 cgcttcgagt accacctgga atgagttgag ataaccatac aggcgcgttc ctacatcgtg
     2401 tgattcgaat aaacctgcat aaaagacagg aaaaagttgt tgatcaaaaa tgttatagaa
     2461 agaccacgtc ccaacaataa atatgacgaa aacccagaag tttcgatcct tgaaaactgc
     2521 gataaaatcc tcttttttta cccctcccac atccgccgct acgcactggt gatccttatc
     2581 tttaaaacac atgttgatca tcataaatac agcgccaaat agcgagacca accagaagtt
     2641 aatatgggga ctgatactaa aaaatatgcc ggcaaagaac gcgccaatag catagccaaa
     2701 agatccccag gcgcgcgctg ttccatattc gaaatgaaaa tttcgcgcca ttttttcggt
     2761 gaagctgtca agcaaaccgc atcccgccag ataccccagg ccaaaaaaga gcgcccccag
     2821 aattaaacct acagaaaaat tgctttgcag taacggttca taaacgtaaa tcataaacgg
     2881 tccggtcaag accaggataa aactcataca ccagatgagc ggtttcttca gaccgagttt
     2941 atcctgaacg atgccgtaga acatcataaa tagaatgctg gtaaactggt tgaccgaata
     3001 aagtgtacct aattccgtcc ctgtcaaccc tagatgtcct ttcagccaaa tagcgtataa
     3061 cgaccaccac agcgaccagg aaataaaaaa gagaaatgag taactggatg caaaacgata
     3121 gtacgcattt ctgaatggga tattcagtgc cataattacc tgcctgtcgt taaaaaattc
     3181 acgtcctatt tagagataag agcgacttcg ccgtttactt ctcactattc cagttcttgt
     3241 cgacatggca gcgctgtcat tgcccctttc gccgttactg caagcgctcc gcaacgttga
     3301 gcgagatcga taattcgtcg cattcctctc tcatctgtag ataatcccgt agaggacaga
     3361 cctgtgagta acccggcaac gaacgcatct cccgcccccg tgctatcgac acaattcaca
     3421 gacattccag caaaatggtg aacttgtcct cgataacaga ccaccacccc ttctgcacct
     3481 ttagtcacca acagcatggc gatctcatac tcttttgcca gggcgcatat attccgatcg
     3541 ttctgtgttt ttccactgat aagtcgccat tcttcttccg agagcttgac gacatccgcc
     3601 agttgtagcg cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca tagatcttca
     3661 cgaatattgg gatcgaagct gacaaaacct ccggcatgcc ggatcgcagt catcgcagta
     3721 aatgcgctgg tacgcgaagg ctcggcagac aacgcaattg aacagagatg taaccattcg
     3781 ccatgtcgcc agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact ggggcggacc
     3841 ataaacgtaa atgaacgttc cccttgatcg ttcagatcga caagcaccgt ggatgtccgg
     3901 tgccattcat cttgcttcag atacgtgata tcgactccct cagttagcag cgttctttgc
     3961 attaacgcac caaaaggatc atcaccgacc cgacctataa acccacttat tccgcctaat
     4021 ctggcgattc ccaccgcaac gttagctggc gcgccgccag gacaaggcag taggcgcccg
     4081 tctgattctg gcaagagatc tactaccgca tcccctaaaa cccatacttt ggctgacatt
     4141 tttttccctt aaattcatct gacttacgca tagtgataaa cctctttttc gcaaaatcgt
     4201 catggattta ctaaaacatg catattagat cacaaaatgt catagttaac gttaacattt
     4261 gtgatattca tcgcatttat gaaagtaagg gactttattt ttataaaagt taacgttaac
     4321 aattcaccaa atttgcttaa ccaggatgat taaaatgacg caatctcgat tgcatgcggc
     4381 gcaaaacgca ctagcaaaac ttcatgagca ccggggtaac actttctatc cccattttca
     4441 cctcgcgcct cctgccgggt ggatgaacga tccaaacggc ctgatctggt ttaacgatcg
     4501 ttatcacgcg ttttatcaac atcacccaat gagcgaacac tgggggccaa tgcactgggg
     4561 acatgccacc agcgacgata tgatccactg gcagcatgag cctattgcgc tagcgccagg
     4621 agacgataat gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct
     4681 ctcacttatc tacaccggac acgtctggct cgatggtgca ggtaatgacg atgcaattcg
     4741 cgaagtacaa tgtctggcta ccagtcggga tggtattcat ttcgagaaac agggtgtgat
     4801 cctcactcca ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga
     4861 cacatggtgg atggtagtcg gggcgaaaga cccaggcaac acggggcaga tcctgcttta
     4921 tcgcggcagt tcattacgtg aatggacttt cgatcgcgta ctggcccacg ctgatgcggg
     4981 tgaaagctat atgtgggaat gtccggactt tttcagcctt ggcgatcagc attatctgat
     5041 gttttccccg cagggaatga atgccgaggg atacagttac cgaaatcgct ttcaaagtgg
     5101 cgtaataccc ggaatgtggt cgccaggacg actttttgca caatccgggc attttactga
     5161 acttgataac gggcatgact tttatgcacc acaaagcttt gtagcgaagg atggtcggcg
     5221 tattgttatc ggctggatgg atatgtggga atcgccaatg ccctcaaaac gtgaaggctg
     5281 ggcaggctgc atgacgctgg cgcgcgagct atcagagagc aatggcaaac tcctacaacg
     5341 cccggtacac gaagctgagt cgttacgcca gcagcatcaa tctatctctc cccgcacaat
     5401 cagcaataaa tatgttttgc aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc
     5461 gctgaagaac agtgatgccg aacattacgg attacagctc ggcactggaa tgcggctgta
     5521 tattgataac caatctgagc gacttgtttt gtggcggtat tacccacacg agaatttaga
     5581 cggctaccgt agtattcccc tcccgcagcg tgacacgctc gccctaagga tatttatcga
     5641 tacatcatcc gtggaagtat ttattaacga cggggaagcg gtgatgagta gtcgaatcta
     5701 tccgcagcca gaagaacggg aactgtcgct ttatgcctcc cacggagtgg ctgtggtgca
     5761 acatggagca ctctggcaac tgggttaaca taatatcagg tggaacaacg gatcaacagc
     5821 gggcaaggga tccgcgtcac acttccccct tcacgacctt caataatatg caatgcagct
     5881 tcccgcccga taatgtcatg tggaagctga attgtggtca gcggcggtaa aaacagatgc
     5941 ccgacgccaa ccagattatc aaagcccatt acggcgacat cctgcgggat acgtaccccc
     6001 ttagccagga gaacctgata agccacaaag gctgcgcgat cgttaccaca tatcagaaca
     6061 tcaaaatctg gtttgcccga tttgaagtgg tcattgagta aacttgcgag atcggtgtag
     6121 tgatcatcac ctgttgccat gtgaaattgt ttcacctcag ccagatctcg tccagcatca
     6181 cgccaggcct gctcaaatcc ctgccgacga taccctgttg ccaacgcact ttccggtagc
     6241 cagaagcata acggttgacg atagcccgcc gcgagcaaat gctgtgttga ttcatattgt
     6301 gcagtgtaat catcagggat ataactgggt aacgctgggt catccgccac acagttcgcc
     6361 aatacaatat tttcaccata cagagactca ggtagcgtga tatgtcgcag ccccattgta
     6421 gtatagataa tgccatccgg acggtgggca agcagctgac gtgccgcgcg ggcagcgtca
     6481 tcttcagaaa aaatattgat taaaaaacta ttccagccga actcgctggc ggtttgctca
     6541 atggcaagca gaatatcaac agagaaagga gtggtagcag tgtcctgcgc cagcacggcg
     6601 agagtcgacg gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc aggaacataa
     6661 ttcagggtct ggattgcctg caatacgcgg tcacgcgttg caggacgcac agattctgca
     6721 ttatgcatca cccgggagac tgtcatcatc gacactcccg ccaggcgtgc gacatccttt
     6781 aatgaagcca tacccaagcc gtttgccgta aaacgggcac tgtagcagaa acagacgtca
     6841 ctggcgagat ccaacgccct atcacctgac acggcaatac aataaaaaat aacaataatt
     6901 cccggacaat tgtccccaat tccgcctctg ttctcgccaa cgagtaacca tataggcaag
     6961 taagtaaaac atgccaactg gcatcgccaa cctggagaag aaaatataaa tatggatcat
     7021 tgtgctgccg ctgtatcccg acatcagccc ggaaattatt gcgattgcta tcggttcagg
     7081 cgcgattggc tgcacgatcg ttacggactc gcttttctgg ctggtgaagc aatattgcgg
     7141 cgctacgctc aatgaaacat ttaaatacta tacgacagcg acatttatcg cttcagtcgt
     7201 cgctctggcg ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg
     7261 gaaaacgcta aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct
     7321 cttaaagaaa ccacctggtt taatcctggc acgacctcat tggctgaagg tttaccttat
     7381 gttggcctga ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc
     7441 tatctggcaa aagcatttcc tgaaactgct gccactgggg ggattattga atcagaactg
     7501 gttgccattc cggctatgca aaaacggctg gaaaaggaat atcaacaacc gatcagcggg
     7561 caactgttac tgaaaaaaga tagccatttg cccatttccg gctccataaa agcacgcggc
     7621 gggatttatg aagtcctggc acacgcagaa aaactggctc tggaagcggg gttgctgacg
     7681 cttgatgatg actacagcaa actgctttct ccggagttta aacagttctt tagccaatac
     7741 agcattgctg tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc
     7801 attggcttta aggtgacagt gcatatgtct gctgatgccc gggcatggaa aaaagcgaaa
     7861 ctgcgcagcc atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag
     7921 gaaggacgta aagcagcgca gtctgacccg aactgtttct ttattgatga cgaaaattcc
     7981 cgcacgttgt tccttgggta ttccgtcgct ggccagcgtc ttaaagcgca atttgcccag
     8041 caaggccgta tcgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc
     8101 ggtggtcctg gtggcgtcgc attcgggctt aaactggcgt ttggcgatca tgttcactgc
     8161 ttttttgccg aaccaacgca ctccccttgt atgttgttag gcgtccatac aggattacac
     8221 gatcagattt ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgca
     8281 gttggtcgcg catcaggctt tgtcgggcgg gcaatggagc gtctgctgga tggcttctat
     8341 acccttagcg atcaaaccat gtatgacatg cttggctggc tggcgcagga agaaggtatt
     8401 cgtcttgaac cttcggcact ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta
     8461 agttaccaac agatgcacgg tttcagcgca gaacaactgc gtaataccac tcatctggtg
     8521 tgggcgacgg gaggtggaat ggtgccggaa gaagagatgg agcaatatct ggcaaaaggc
     8581 cgttaataac atttcaacgc agcatcgcca tcctttccct gggtgagcga tgctgccgat
     8641 ggcgcagact taagatcccc ggtcttaccc gctataaccc cctgctttta ccatcaccca
     8701 acgcctttcg ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag taagataaaa
     8761 gcaatagccg ccatacggaa aatctcattt gcagaaatag aaagtgactg ctgggttatt
     8821 tcattattaa tttcattaag aactcctgaa agcgaaccat agtatttatc cataatttgt
     8881 gacgatgaat taaacacagg gttaaattga tcgatggttg ctgtcaactg actatggtgt
     8941 aacgattcgc gtcgccccca cagcgtcatt gtcaacgacg taccaactga tcctgacaag
     9001 gtacgaaaaa aattactcat actcgaggca ttggcaaatt tattatctgg caaccctgaa
     9061 aacgaaatcg ttgttaaggg taaaaagaaa caggcaacgg cgaacccctg gaaaaactgt
     9121 ggcaaaatga tgcctgtaaa atcaatcgtt ggcataaatg tcacagaacg ccagtaatag
     9181 caaaccgcat acatcaaaaa actaaatgtc actaacaacc gcatgtctat tttgttgcca
     9241 taacgtccta tcaaaggtga aattaatagt ggcatgatgc cgatgggcgc ataagcaagt
     9301 ccggcccata tcgcattata ccccatcgtt tcctggagta actgcggcat aaggacgatc
     9361 gctccagagt aaaataaata cgcgcatgtg atactcacta taccaatggt gaaattacgg
     9421 gacttaaaca aactgagatc aagaatcggg ttctctgagg tcgactccca aatgactaaa
     9481 gagatcagag aaataactga tactactgtt aatattatta ttgtactcga gttgaaccaa
     9541 tccagatcgc gccctttatc aagcataatt tgcaagccac caacaccgag cactaacagg
     9601 gtcagtcctg gtagattcat tttgaccggt gaagtctcag tttctcttcc tttaagtaag
     9661 gttaagcata atgtcaggac gataataccc atagggacat tgattaaaaa tatccaaccc
     9721 cagctaaagt tatcgcaaat atatccgcct aatatcggcc cacatatcgg agctataatc
     9781 acggtcattg accataatgc cagagcaaat gttctttttt ctggtggata attccttaat
     9841 aataaactct gtgacagtgg aattaacggc cctgccatta acccctgaac gactctaaaa
     9901 aatatcaaca catcaagatt ggtcgataaa ctacacatta aggaagacag cgaaaaaaaa
     9961 gtgactgaaa gtaaaaataa ccttaattcg cctattcttt gtgccagcct gccggtaaca
    10021 gggatcgcaa tggcatttgc tacgccaaac gaggtgataa cccaggtgcc ttcgtctgtt
    10081 gatgctccca gaaagccaga tattgtcggt attgcgacgt tagaaatagt ggaatccaac
    10141 atttgcataa acgtcgctaa tgacaatgca atagtgacgc accataacgt cccaccggtt
    10201 aatggtgctg gagttgattt agtgattgcc attaaagttg tccattatgc gaaataatgt
    10261 tgctaatttc tttttctatc ggactggtat cgataactaa agccttactg gtataagcag
    10321 gcatggaggt cacggttgaa gccagatcag gcatctcagc aatgtcttcg tccttcgtat
    10381 caatagttgc agtcatcgat aaaccaatgc gcaaggggtg ttccatgagt tcttttggat
    10441 caagagaaac ttcaaccggt acacgctgaa cgattttgat ccagttccct gtcgcatttt
    10501 gtgcaggtaa taaggagaac gcattgccgg ttcccatatt gatccctgtc acccggccat
    10561 gaaacacaac attttcacca taaagatcgc tgataatatt gaccgattga ccaatccgta
    10621 catccgtgag ttgtgtttct ttaaagttgg cattaaccca catttgacgt gctggtacga
    10681 cagccattaa cgattgtccg ggactcactg tttcgccgac ctgaacactt cgctgggcaa
    10741 tatagccggt aaccggactc ttaatatccg tacgtttaag cgccaaccag gcttctttgg
    10801 ttgcatccgc agcttcaacg acttgtggct gacggtttaa tggtgtgttc attactaaag
    10861 ctttattcgc tttataagcc tggatagcgg cattcaatgc cgctttgcta cttattaacg
    10921 tatctttggt atgctccagc gtttcttttg aaataacccc ctgcttcgct aaaggcacac
    10981 gacggttata atcttctaaa gattgttgat actgaatacg tgctgaagcg acttcggcac
    11041 tgtattgttt atcctgtaag tacagtttat tcgtttgccg aacaatattt gccagattat
    11101 ttttagcttt attgagtgcg atagtggcat cagttttatc cagtgaaact aaaatgtcac
    11161 cctgtcgaac gtagttcgta tctttatgat taacgacagt gacactacct gagacttgtg
    11221 cagaaattgg atctgcattc cccgtgacat aggcgtcatc tgtactaatc atgtcttcta
    11281 attccattga ccaataggca taagcacctg aaaacgcaat aaataaaact atcgccaata
    11341 aagaaaagta ttttcttctg ttagagtgtt ttttatttga attaatctgt tccactatta
    11401 tctctcattt cgcatagatg aaatttatga attgaatgaa aagagtattc attcttcata
    11461 tcaaacacag tgcatgtgtt gcacattatc ccaggaacgt tggaggccta atttaaatga
    11521 ggtgcaaaaa aaacaacaga ggactaaacc gtggcttttg caatacaatt cttacgcctg
    11581 taggattagt gagaagactt atagtgctaa tgtgaaacta taaatcatcg gtataatccc
    11641 tgattttatt gttgacattt catatatgcc gactatttat atggtatact tatcgaatta
    11701 tcttaaagga agctcaaatt ttcttatttt tattgagaaa atgagatgat gccttatgtc
    11761 tgtattacta cagggagaag ggagatgctt cattgcaaag ggaataatct atgaacgcaa
    11821 taattattga tgaccatcct cttgctatcg cagcaattcg taatttattg atcaaaaacg
    11881 atattgaaat cttagcagag ttgactgaag gcggaagtgc cgttcagcgg gtggaaacac
    11941 ttaagcctga tatcgtcatc attgatgtcg atatccccgg agttaacggt atccaggtgt
    12001 tagaaacgct gaggaagcgc caatatagcg gaattattat tatcgtctcc gctaaaaatg
    12061 accattttta cgggaaacat tgtgctgatg ctggcgctaa tggtttcgtg agtaaaaaag
    12121 aaggcatgaa caatatcatt gcggctattg aagctgcaaa aaatggctac tgctatttcc
    12181 ccttctctct caaccggttt gttggaagtt taacgtccga ccagcaaaaa ctcgactcct
    12241 tatcgaaaca agaaattagt gtcatgcggt atattcttga tggcaaggat aataatgaca
    12301 ttgctgaaaa aatgttcatc agcaacaaaa ctgtcagcac ttataaaagt cgcctgatgg
    12361 aaaaattaga atgtaaatca ctgatggatc tttacacatt cgcacaacgt aacaaaatcg
    12421 gctaaccaca tgaagttttt accctatatt tttcttctct gttgtggtct ttggtcgacc
    12481 ataagtttcg cagacgaaga ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc
    12541 acacttgatc cactacgtct gagcaacaag gaattacgtt ggttagcgag caaaaaaaat
    12601 cttgtgattg cagtacataa gtcccaaacg gctacgttgt tgcataccga ttcgcagcaa
    12661 cgggttcgtg gtattaatgc tgactattta aatcttttaa aaagagcgtt aaatatcaaa
    12721 ttaacactcc gggaatacgc cgatcatcaa aaagcaatgg acgcgctgga agaaggtgaa
    12781 gtcgatatag tgttatcaca tttagttgct tcgccgcctc ttaatgatga cattgctgca
    12841 accaaaccac tgataattac ctttccggcg ctggtcacca cccttcacga ttcaatgcga
    12901 ccgcttacct catcaaaacc agtaaatatt gctcgagtag caaattatcc ccctgacgag
    12961 gtaattcatc aatcatttcc aaaagcaaca attatctctt ttacaaattt atatcaggca
    13021 ttagcatccg tctcagccgg acagaatgat tactttattg gtag
//
LOCUS       DAZFFG010000125        12610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFFG010000125 DAZFFG010000000
VERSION     DAZFFG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.125"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..148)
                     /locus_tag="ACHJ7J_004075"
     CDS             complement(<1..148)
                     /locus_tag="ACHJ7J_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5002245.1"
                     /translation="MTKYALIGLLAVCAKVLCFSLIFRERLCELNIHRGNTAVQVTLA
                     YEARK"
     gene            complement(427..1017)
                     /locus_tag="ACHJ7J_004076"
     CDS             complement(427..1017)
                     /locus_tag="ACHJ7J_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO5002246.1"
                     /translation="MKLIIFILIILIIAALLIRIILGSVNQHSPLLMQLHVAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(1055..1264)
                     /gene="hha"
                     /locus_tag="ACHJ7J_004077"
     CDS             complement(1055..1264)
                     /gene="hha"
                     /locus_tag="ACHJ7J_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5002247.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(1310..1771)
                     /locus_tag="ACHJ7J_004078"
     CDS             complement(1310..1771)
                     /locus_tag="ACHJ7J_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5002248.1"
                     /translation="MRKYIPLVLFIFSWPILSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPKKKQDYGRWSTGMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTEPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(2014..2226)
                     /locus_tag="ACHJ7J_004079"
     CDS             complement(2014..2226)
                     /locus_tag="ACHJ7J_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO5002249.1"
                     /translation="MNGFRNSSRNGQVWRYQLAGGQAVILEISGRWMEAAEAWQRAAC
                     IAPRTDWQQFARKRAEHCHLRCRGKV"
     gene            complement(2362..2922)
                     /locus_tag="ACHJ7J_004080"
     CDS             complement(2362..2922)
                     /locus_tag="ACHJ7J_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO5002250.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAARIAELTAKKAQARQGLSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLARGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            complement(2977..3723)
                     /gene="traX"
                     /locus_tag="ACHJ7J_004081"
     CDS             complement(2977..3723)
                     /gene="traX"
                     /locus_tag="ACHJ7J_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO5002251.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAYIRQSAINRLWGWAMIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCDTRSLWRSAGTMLLLAVWLPLSGTSYGVAG
                     LLMLAVSHRLYRAEDRTERLALVACMLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPRDFFPTFYACHLAVLGVLAL"
     gene            complement(3743..9013)
                     /gene="traI"
                     /locus_tag="ACHJ7J_004082"
     CDS             complement(3743..9013)
                     /gene="traI"
                     /locus_tag="ACHJ7J_004082"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO5002252.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRILEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGRDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEVFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAATRTQTPGPVSQDGQDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELAMMAREHGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GQQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSNSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKISGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLESGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISHQ
                     KSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTDLGREINAQIKR
                     GDLLYVDVAKGYGTGMLVSRASYEAEKSILRHILEGKEAVTPLMERVPGALMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPAQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            9095..9322
                     /gene="vapB"
                     /locus_tag="ACHJ7J_004083"
     CDS             9095..9322
                     /gene="vapB"
                     /locus_tag="ACHJ7J_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO5002253.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGNSVSADFMDNREQPGMQERESF"
     gene            9322..9720
                     /locus_tag="ACHJ7J_004084"
     CDS             9322..9720
                     /locus_tag="ACHJ7J_004084"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO5002254.1"
                     /translation="MLKFMLDTNICIFTIKNKPARVRDRFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRIEDWS"
     gene            complement(9729..11927)
                     /gene="traD"
                     /locus_tag="ACHJ7J_004085"
     CDS             complement(9729..11927)
                     /gene="traD"
                     /locus_tag="ACHJ7J_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO5002255.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGRDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCVAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGIS
                     ESGEVVDMAAYEAWQQENHPDTQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(12172..>12610)
                     /locus_tag="ACHJ7J_004086"
     CDS             complement(12172..>12610)
                     /locus_tag="ACHJ7J_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002256.1"
                     /translation="TGGSDTYFLYTPALFVDKQVAAPSVNVENLCAISGGQRRIKKFT
                     SDTFIESGYKVAKAFRDMGLYAHFGAYEAFRDSYQSFEKQKIAYRFQTEKLDEYVNKG
                     YFFDDFSCIVNGKEIWSLSIEPVAVDQDSSGVDQIWMLMTVNS"
BASE COUNT         2760 a         3801 c         3015 g         3034 t
ORIGIN      
        1 acttccgtgc ttcgtaggcc agagttacct gcaccgctgt atttcccctg tgaatattca
       61 gttcacataa ccgttccctg aatatcagtg aaaaacacaa caccttggcg cacacggcga
      121 gcaacccgat aagggcatat ttcgtcataa actttgacct ctctgcaagg aagaggctac
      181 aatcagtagt gcaacgtact tcatgcagac ctcacgagtt aatggattaa caagtggggt
      241 cttcgcattt ctggtacctg cgtcttgcct gaaccagaaa ggctcagaca cccgccgcga
      301 taataccaca ttccctgtta ctgaccattc cctccatttc ccgcttttca ctacagaaac
      361 gccggggacg gggaaaactt gctttttccc gtttccgggg ttggacaact gagcaacgcg
      421 aaggcgtcag ctacgatgtt ccggtgactg ctgatcagcc cctgttgtat tcaaccattc
      481 tccggtcatc tgcagcagtt ttctggcatc gtggcttctg agcaacggaa taccagcctg
      541 cctcagaacc tcttccagaa gaatatcccg gcgtctgcgt tccggtcgta aatggctggc
      601 atcatccagc tctactgctg caacaatact gaacgaacgc cgctcaacga taaccacatc
      661 aacatgccac tgagacacca tcctgaataa ctgccaccac tggcgtgatc gtggccggat
      721 attcccgttc agctggacga tatccgcgac ccgtacctgt ggacacagat accaccgctt
      781 catatccaca attctgaaca gtcctttcat aaaactgact tccctttctg tcagcagcgt
      841 cttctgacgt tgccagtatt caccgctgga cagtattcgc tctgcatcac cggtccgtat
      901 acctgccaca tgcaattgca tcagcaaagg agaatgctga tttacacttc cgaggataat
      961 ccggataagt aacgctgcaa taatcagaat aatgagaata aaaataatca acttcattat
     1021 ttatctgctc ctggcaatta attatgctga attattacct tacatatttc catatctctt
     1081 ttggtatcct gtcatataac tttccggtga ttaactccgc taaccggtgg tctgctgctg
     1141 aattaaatga ttcacgttca ctgtttgata aactgtctct gtttttatga ataatttttt
     1201 ccagtgtatt gactgatgaa catcgccgta actgatacag ccattcctgt tttgtttttg
     1261 ccatttttat tcccgctgag aacagatgaa tgataagatt gccatcgctt tatttacggt
     1321 gtctccatat ccagggggga accggatcag catctgacca gagcccacgt ttttgttgtc
     1381 tggcttcgtt ctgaaggaca ggcagcacgg gctctgtgtt gtactgctca tatacccagg
     1441 cagccccggc ccggaccata aactgattaa tattcattcc gtctggtgca taaacctgcc
     1501 cgagaatacg accataacga tctcgctgaa aatatgtgac ggtaaccgtt tttcctgcaa
     1561 ccagtgattt catcatgccc gttgaccacc ggccataatc ctgtttcttt ttcggtgcat
     1621 caatatttac cagcctgatc cgcaccgcct tcagtgagtc cattacctca atggtatcac
     1681 cgtccagtac acgaacaacc cgtccatgaa tatctgcaga caatatcggc catgaaaaga
     1741 taaataatac cagtggaata tatttcctca tgttattctc catatcaaat aaatggcacc
     1801 tgtatttcag acaccctgaa tattgcagca cacattgaat aacaccttat tttgtgaaat
     1861 taatcacaaa atataaaatc acggcttaat acggaaaaaa ctctgtgcta ctcagaattg
     1921 tccggtcata ctgcccgacc ggagattaaa aaagtaaatc tcatgcatta ttcagccttc
     1981 tgactcctgg agtaatgaag gctgcttttt tcgttacacc tttccccgac agcgcaggtg
     2041 gcagtgttca gccctttttc gggcaaactg ctgccagtct gtccggggag ctatgcaggc
     2101 agcccgttgc catgcttctg ccgcttccat ccagcgtcca ctgatctcca gaataacggc
     2161 ctgcccgccg gcaagctggt agcgccagac ctggccgttc cgtgaactgt ttctgaatcc
     2221 attcatcttt atcactcctc tttaacccgt cagtattatt gtggtaaata attcagagga
     2281 ctggcttcct ttcgggtatg gaagttattt ccttttaagc cttattgttt cagcctcctc
     2341 actctttcgg ggaggctttt tttatttctc atcaagcaca gcctgaagct ctgcctttat
     2401 ccggttctgg cgacggattt tatccagacg cgctgccgca tacgcttcct cctcctgaga
     2461 aatatgctca gtcacatacc cttccgtgtc ataccggcag gcaccggctt tcatggcaca
     2521 cagatagctt tctgaacggg tgatggcctt cagcgccctg cgcagttttt tatgcgagag
     2581 cgggatattc cgctgcgcca cgtcttccag taacacgtcc cggataccgc gggccagaag
     2641 ccggggggtg tcaccgtcaa ataatccggg ccaccagggc ttcagggtat tcacggcctc
     2701 atccagcgtg ggcaggttca gataaatgga cagcccctgt ctggcctgcg ctttttttgc
     2761 tgtcagctca gctatccggg ccgccttctc ggccaacttc tgctttttca ccttccattt
     2821 tggtggcgtg gtgacattga tgatagtttt ccggctgcgg actggcgtct ccccttccgt
     2881 tttccgcttc agtgtcagta ccggtctctt ctgctctgtc atgctgtgtt ctgcctctga
     2941 cgaatcatcc tgttacggcc aaagatacca cacccgtcac agtgccagaa cgcccagcac
     3001 agccagatga caggcataga acgtcgggaa aaaatctctg ggccagaaac ggggtaatga
     3061 tttccctgca cacgacacca gaccaacggt cagcaccgtc atcacaaggc cagccaccgc
     3121 cgccgcatca ctggtggcaa ggttaagcgc cggaatcacg gccagcatgc aggcgaccag
     3181 cgccagacgt tccgtcctgt cttccgcccg gtacagccgg tggctgaccg ccagcatcag
     3241 cagcccggca acgccgtaac tggtgccgga caaaggcaac catacagcca gaagcaacat
     3301 ggtccctgcg gaacgccaca aactgcgcgt atcacaccac gtcagcacct gtgccgccac
     3361 tgcaaaggca aacaggatat tcccctcata ccagggaaag ccggccagat aatatgcgaa
     3421 ctgggcaatc attgcccatc cccacagccg gttaatggcc gactgccgga tatacgcatg
     3481 gcgggacaga ttcagccccc ataccagggc aaaaagcgga aatgccccac gcccggccag
     3541 aaacatccac gtctggtcca gatgcagaat gcggtttgca tggtccagta ccatcagcac
     3601 cagtgccacc gttttgatga tatcccgctg tccgggtgac cagaccagca acgactgcaa
     3661 ccaggtatcg gtccgggcag ccagtgaatc gttacgtgtc gtgttcgtgt tatccgttgt
     3721 catcagcgtt tgtcttcctg tatcagtccc cgcccagggt tttctctttc tgcaggtcac
     3781 gaaccatatc ccgctccatc tgctgcagac gctcctgcag cagattttcc cgggcaacct
     3841 cccggaccgc ttcccgctcc cgttgtgatt cacgcagcac actctccggc agtgcggctt
     3901 cccgttcagt tatggcagcc cggtcacgct cctgcccggc aatttccctg acagcctgct
     3961 ctgttttgcc atccggcagg tcaggtttgt tctctgccat tttacggaca atttcatctg
     4021 cacggcgttc cgtttcacgg cggatggcct cttcagcctg ccgctgtgcc agtacctctg
     4081 ccgtgaccgg ttcaccgttt ccggcacccg gctggacact gttgtccggg atatcccccc
     4141 acacgcgacc gccggtgatg gcaccgggat tccacgggcg accttcaccg gcaattctca
     4201 ccaccacacc actgtcagga ttatcacggg caatccggac gccatcctgc atattatcag
     4261 ccagcaggct ctctccgtta cggctgccct gcagggccac gaactgcgca tccccgctgc
     4321 ctttcacccg gccttcaccg ctgaatcccc gcagcccgtt tccgtcatcc gtggtcagcg
     4381 ggttcagcca gataccggca gacttgccgt tacggtcaaa cgccggcagt gccacatacg
     4441 gttgcggata tttacgtccc ggagcaataa accgtgcagg actgtctccc ccggccagcc
     4501 ccgcctgacg gagaacggca cggccagctg ccacgtcccg cagctcccgc gccgtactga
     4561 acagccgctc tgcattcatg acctcccggt ccgatttcgg ctccagcaca tcgtgggcag
     4621 ttcctttctg tacggcattg ttaatggcat ccgtccagcc ctgacggtta tcggtgtaca
     4681 cctgcacatg ctgcttcata cgcgacaggg ccacgtaggc tgactcaaag ccggccatct
     4741 gtttccggtt accttccgtg ccttcaagcg caatggcaaa ggtttcactc gccccctgcg
     4801 caccgtgagc ggtgatggca taggccaggt caatatgttg ctctgcccgc tcctgggcgg
     4861 ggcgaatcac ccgggtctgc tgtccgtccg acagcgtgac actgtcaccg gaaaccgctg
     4921 tcaccgtcca gacgctgttg gccacataac cacgctcccg gtcactcttc gtgaagcgca
     4981 tccggtcacc ggtccccacc cggatggtgt ccggggtgta cagtgtgacg ccttcagcca
     5041 ccgcctcccg gggcgaaatc agccgcgtgt taccttccgc atcctgcagg gttatcagcc
     5101 cgtcatcctt actgataccg gcaatccggt gatacacatt atcaaccagg acaagtgcgt
     5161 cccgatgtgt ctcccaggtg gagagacgac gcagctctcc gtcgcgtata ttcgctgtgt
     5221 tcaggacagg caccatgacc tgctctttcc ccagctcacc ggccttttcc cgtgcatcat
     5281 gaatcatgct gttcagtacg cgcctgtctt cattcaggtg cgtgacaatc agcgtctgct
     5341 cccgtgcttc cggtgttctg ccggtatagt cgcggacaat ggcttcatac agtgtcatgg
     5401 ggacatccgg gaaagcctcg cctttcagca tcgccttctg ctgcgcttct gccagtttcg
     5461 cttcctggct gtgactgaac tccgtcacag agtgctccgg tgcccatgct ccctccagac
     5521 gtggcacctg agacggtttc acactctcaa gtccggacag cgccctttcc acatcccggt
     5581 taatcaggct gtataccgcc tcccgcagtt ccggcgtctg acgcacaatc tccttcatga
     5641 tggccacatc ggcagcactg cgcgtctgct ggagacggaa aggctgaccg ggcgcgatgg
     5701 cctgcagctg gtccgtgtca ccgctggcca cggcacgacc accaccggcc gcaatcaggg
     5761 cgtatgcccg tgccatgtcg gtattgccca ccattgagct ctcatcgagc aggaacagcg
     5821 tgttgctgaa atccggcgtt tctccgctgc gctgctgcag ctgcgtgtca tgcagaaatg
     5881 acgccagtgt ctgtgcatcc acgcccgcgc tgcgcatctc cccgaccgcg cggtgcgtgg
     5941 gacccagccc aacgacacgg ggacgctcac tctccggcag catgttcacg gctgacatca
     6001 ccgcccggaa ctgtgtggtc ttacccacac cggcataacc ctgcaccacc gtgaaacggt
     6061 cggacgtttc cagtatcatg cgggtggcgg cacgctgccc cgacgtcagt gtctccatga
     6121 gtgcgccggg taccctctcc atcagcggcg tgaccgcctc cttaccttcg agaatatggc
     6181 gaagaatgct tttttcagcc tcatacgacg cacgggaaac cagcatgcct gtgccatagc
     6241 cttttgccac atccacatac agtaaatcac cgcgttttat ctgcgcattg atttcccgtc
     6301 ccaggtcagt aaaactggtt ccttctgcag caaacgactt cgcctctgtc agcaggtcca
     6361 ccatgctgaa ggccaggttt ttactttcca gcacaggaag ggccagatga atggcctgct
     6421 gtgccggcgt gtgaagtgcg gacttctggt gactgatagc ggtttccagc gatgtctcac
     6481 cggcacgcgc ctttatctgc tcagaaacca ccgtaaagga cggatgacgg gcaagttttt
     6541 ccgcagtacg ggtttcatcc agtgaggaat aaagccggac atcacgaccg ctgcgggcca
     6601 gaccgttcag ggtggcgttg tccattgcca tctgtgtgac ggaggcaaaa accttcgcac
     6661 tgtcgctgac ggaatgcccg ggcgtttcca cccagccgct ctccagcttc agtgccgtga
     6721 atggtgaatc gctcacaggc agggaggccg gttcagcccg ccccggcaca acaaccgtca
     6781 tcgcgtcctc gctgacggat gccacctgca ggcggtcacc gccggagacg cgaagtccgg
     6841 aaattttccc tgtcaccctc agacgctcgc cgtctgccac cggcattttt tccggccgga
     6901 acagcgacca gctgctgtcc agggaggaaa tacgcaccac ctgtgtttcg ccctgcgcat
     6961 tccgcagggt caggctatta ctctgcgccg tcacacgttc tgtcacatag cggtcatgac
     7021 tgcgtgtctc cgggttccac tgctccatca ccatccccgg tcggtacata tcccgcagat
     7081 aacggctccg gctgtccagc cagaccggtg acagggcagt catggtcacc tcctgctgtc
     7141 cgagcacgcc ctgtgttttc agctcactgc gaatggcctg tgtcagtatg gcctgttccc
     7201 gtaccccgct gacctgtgcc acgctctctt ctccggcttt cacgctagcc gcaaaatctc
     7261 cggccagccg ggcatagcgg acattacggt ccggttcact gatgatggtg gccggtcgct
     7321 gttctccccc ctgccagcga tatgtgttca ccccggcatc tttcatggcc atcagtgcac
     7381 tgcctgtacc ggttcgctgt ccgctgtcgg ttatcaggac ctgtacgtta tgacgtgcgg
     7441 caccgtccag cagggttaac gtctctttca gggaaagttt ttcgccctgg tcaacgataa
     7501 cggtactgcc cggcgtgaag gccatgccct cctgcagctg acgacgtccg gttatcagct
     7561 caccggacag ccgttcatcc tgcttcaggt tcatctgtga gcggcggtca gcggcgataa
     7621 tctgcacctc ccgcccgtgc tcccgggcca tcattgccag ttcagccacc cgctcacgct
     7681 gcccggctgc accgccctgc ccggacacaa tggccagcga cgggcgatcc tgtgccagaa
     7741 cactgacggc atcgctgtaa ccggccgtcc gggggacact tttctccgga tgtacggtca
     7801 cccggttctg tttcatgatg tcacgactga gtgcccggac tgacagctca tcgagcacat
     7861 gaatcccgga cgtgaacagc cccttctcac gatcgagggg gataagctgc tcacggctga
     7921 tggcctcatc gataccggcg cgtgcccgtt caatcacacc attttccggc ggcaatatgc
     7981 cgaccgttct ggccagcacg tccgtgtacg taaactgcac tttgcgttca cttaatccgg
     8041 caatcgcttg tgtcaccgcc tgctgcacat cctgtccgtc ctgtgaaact ggcccgggcg
     8101 tctgcgtgcg ggtagcggcc cgttgatccg ccgcgtcacg atatgcccgg atgtcgaacc
     8161 cggtttcctt cagcgtctgc atccattcag ccattctgac ttcaggatcg acgtgctgtt
     8221 tggattttcg tgtatccagt gccgccacat cccgggattt cagcgaggcg tcctccccga
     8281 cggcctcccg gatagtctgt gagcgtccgg aaaagacctc caccggtacg cccggcattt
     8341 cccacatacc gtgcttacca accacttcag tttcatagcc cagcgcctca acctgctctt
     8401 tcagtttttc ccggtagagc ctgccaaagg caatctgatt agcgtacaca ttctcaatga
     8461 accccgtttt ccccactttg tcactgctca gtgtcttcca ctcgccgtta tgctgcgtga
     8521 cattagccac caccgcatgc gtgtgtaact gtggttcctg atcgcgactg gtgtcgtgat
     8581 taaaaagcgc catcaccaga ttaccggtca gcaccgtttc tgactgtccg tccgtcataa
     8641 cccgtgtgga ggccagtgcc tccacctgac ggacagcaaa atccacagcc tggttatgtg
     8701 catcaataag acgtttgtct ctcccgagca tggccatcat ggagacactt ttgggggcgg
     8761 agaaggtcag gtcgtagccg ggacgatgct tattactgcc atcctgcatg cggcttaaat
     8821 ctgctccgtc cggcagcttc ccctccagaa tacgggtaaa aacatcctta tcgacactgc
     8881 cctgcagccc cagctgttct gccccctggc cggcccagcg ttctcccatg ctgcccagca
     8941 catagtaatt atccttgtcg gtataatagt tcccggcact gccggccgat ctgacctgcg
     9001 cgatactcat cataaacgta tatccctttg acatatcccg gcatcaatcc cacaatggat
     9061 atacacaaga catatccaca taaggaggca aataatggaa accaccgtat ttctcagcaa
     9121 ccgcagccag gcggtcagac tgccaaaagc ggttgcattg ccggaaaacg taaaacgcgt
     9181 tgaagtgatt gctgtcggac gaaccagaat cattacgcca gccggagaga catgggacga
     9241 atggttcgac ggaaacagcg tcagcgccga ttttatggat aacagggaac agcccggtat
     9301 gcaggaaagg gagtcattct gatgctgaag tttatgctcg ataccaacat ctgcattttt
     9361 acgataaaga acaaacccgc ccgtgtcagg gaccgtttta acctgaacca ggggagaatg
     9421 tgtatcagtt cggtcaccct gatggagctg atatatggtg cagaaaaaag ccagatgcct
     9481 gaacgtaatc tcgctgtgat cgagggattt gtttcccgca ttgatgttct ggattacgac
     9541 gctgctgcag ccacacacac tggccagata agagcagaac ttgcccgtca gggacgccct
     9601 gtcgggccat ttgatcaaat gatcgcaggt catgcccgca gtcgggggct gattattgtg
     9661 actaataaca cccgggaatt tgaacgtgtg ggcggcctga gaattgaaga ctggagctga
     9721 cctgttcatc agaaatcatc tcccggctca acatcctccc cgcgctcccg gtgcacattg
     9781 atgttcacct cttcacgacg ctgcatctgc tgctgggtgt ccggatgatt ttcctgttgc
     9841 catgcctcat aagcggccat atccaccact tcaccggatt cactgatccc gggtggcagt
     9901 tgctgttcca tctcttcttc cggtttcatt ttcagctcct gctcgatccc cccagccgga
     9961 acactcacac ctgcatctga cttcttatca ttgatgacag aagacactgg ctgttgcggc
    10021 tgttgcggct gttgcggctg ttgcggctgt tgcggctgtt gcggctgttc agcctgagtc
    10081 acgtcttctc cggaaacaac ctccgggaca tccggttcga agaggctggc catctgacga
    10141 ccttctgctt cccttgcggc aagtacagca ctcagacggt tctccatttc cggattgatg
    10201 tcacgcggaa taaactccgg agcgaccttc ggtcgtgcct gatatttcag tgagagtttt
    10261 actgccggat acggtccggg cagggtgaca taacaggtca gatccggcag agactgaatg
    10321 tcggaataac tgaccagcgt ctggcgctcc atatccttac cggtcgatac cccgtcacgt
    10381 accgggtcag caccatagga atactgctcg ctggctttca ggtgctcttt ctcaccaatc
    10441 tcaccggcag cgaactctgc aatcttatgg ctgggagaac ggaaaaaagc acgggtgttc
    10501 atgacgtcaa acagcgtggc cgccgctttc tcaccgtaga tatcttccag ctgggcatag
    10561 gactggatac caaacacata acagccaccg aacttacggg cttccggcag gatctccacc
    10621 aggtccggca gtttgtgtaa cgtgggtagc tcatcacaga aaaaccacac acgacggtta
    10681 cggttttctc ccattgccag cagaccacga atggcaatgg acagccacat ggagatcacc
    10741 ggcttcaggg aggcatgggt gtcggcattt gacgagataa acagccagcc gtttttctga
    10801 tcttcccgga caccccgcat ccagtcacgg atggtaaaag actcaccgtt atgctcaatc
    10861 ccctgcaggt aacggatggc tttcacgtag ttggtcagca cagcacggat ggaaatcgcc
    10921 gttttctcaa ttttctcttc caccaggttg gccgccggtg aattacgcag gaaggtacgc
    10981 agtttttcga ttttgatgga aagcagtgtg tccaccagtt tgctgtagct gcggttgggg
    11041 tcattacgca tcaggtacgc cgcttccgca aaaatggtac gtcctgaacc ctgccagaac
    11101 gggtcctctt ttgtgcccat cgggatcagc gtgttcgcgg tattatcaaa atccggctgt
    11161 gtcaggcact ccttccacaa atcccaggcg acacagcgtg catccagcgg attcaggatt
    11221 ttatcgatgg agggatcata gtaactttta acaaattccc ctgaacggtc ataaatcacc
    11281 accatatctc cacgctgacg ggcgtagttg gccagacgac ggataacttc cgacttaccg
    11341 gccccgaccg tgccgtgcag acagaagttc tggatttcag aatcccggat aatcggcagg
    11401 tcgccaatcc ggatatcgga gtccctgccg tcttttttca gcatccgggc aacgtctttc
    11461 ggattgtctg tcagctgacg accacctgtg acttcatttt cgctctgttg tttaccctga
    11521 cgccccagaa tccaggagac aacaaagaag gtaatcaggc aaataaccag tgccacaact
    11581 gtggccagaa cgaatgcgga ccatagctgc tctccgcacc agatcatata tttatcatgc
    11641 agtacctgag cagcattcat ccggaaggtt ttgccgtaat actggatctc atataccggc
    11701 tgggatttga ttaaatcccg catgccttcc agcgtggtac accaccagta aatacagccg
    11761 ttcacaaacg tctgccagct tatttttacc cataaaacca gaccaacgag tatccagaaa
    11821 aaaataaaca ggcagtaaag catgatattg gcgatctggc tgaacatgcg gatacgcatg
    11881 gacgcaatct gaccgccctg ggtcatatcc tttgcgttaa aactcatgat gatattccga
    11941 agaaaaaagt cagtcggact gattattaat gatgaaaaga ttaataaccg ttaaattccg
    12001 gcttcgccgg caacacttca cggtcgcaca aagaatttgc acaaccgctc agtgttttca
    12061 tatttatttc tccccggggt tggacaacga gcaaagcgag gcgtcagtca ggaggccagc
    12121 aggactggcc cggataattt tcttgacgtc attaaagtta ataaataaag atcagctatt
    12181 taccgtcata agcatccata tttgatcaac acctgatgaa tcctgatcaa cagcaactgg
    12241 ctcaattgaa agtgaccata tttctttacc attgacaata caggaaaaat catcaaagaa
    12301 ataaccttta tttacatact catccagttt ttctgtttga aatctatacg caatcttttg
    12361 tttttcaaag ctctgataag aatccctgaa tgcttcatat gctccgaaat gagcatacaa
    12421 tcccatatca cgaaaggctt tagcaacttt atatccggat tcaataaagg tatcagaggt
    12481 gaatttctta attcgtctct gccctccact tatagcacat aaattttcca cattcactga
    12541 tggtgccgct acttgtttat caacaaacag agccggtgta tatagaaaat acgtatcaga
    12601 accgccagta
//
LOCUS       DAZFFG010000126        12209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFFG010000126 DAZFFG010000000
VERSION     DAZFFG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.126"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            106..963
                     /gene="focA"
                     /locus_tag="ACHJ7J_004087"
     CDS             106..963
                     /gene="focA"
                     /locus_tag="ACHJ7J_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO5002257.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1018..3300
                     /gene="pflB"
                     /locus_tag="ACHJ7J_004088"
     CDS             1018..3300
                     /gene="pflB"
                     /locus_tag="ACHJ7J_004088"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5002258.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3492..4232
                     /gene="pflA"
                     /locus_tag="ACHJ7J_004089"
     CDS             3492..4232
                     /gene="pflA"
                     /locus_tag="ACHJ7J_004089"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO5002259.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            4331..4441
                     /locus_tag="ACHJ7J_004090"
                     /pseudo
     CDS             4331..4441
                     /locus_tag="ACHJ7J_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4442..5872)
                     /gene="ycaM"
                     /locus_tag="ACHJ7J_004091"
     CDS             complement(4442..5872)
                     /gene="ycaM"
                     /locus_tag="ACHJ7J_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5002260.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLSLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(6082..7230)
                     /gene="ycaD"
                     /locus_tag="ACHJ7J_004092"
     CDS             complement(6082..7230)
                     /gene="ycaD"
                     /locus_tag="ACHJ7J_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5002261.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            7546..8172
                     /gene="ycaC"
                     /locus_tag="ACHJ7J_004093"
     CDS             7546..8172
                     /gene="ycaC"
                     /locus_tag="ACHJ7J_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO5002262.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(8208..9071)
                     /gene="dmsC"
                     /locus_tag="ACHJ7J_004094"
     CDS             complement(8208..9071)
                     /gene="dmsC"
                     /locus_tag="ACHJ7J_004094"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO5002263.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAETQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9073..9690)
                     /gene="dmsB"
                     /locus_tag="ACHJ7J_004095"
     CDS             complement(9073..9690)
                     /gene="dmsB"
                     /locus_tag="ACHJ7J_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO5002264.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(9701..12145)
                     /gene="dmsA"
                     /locus_tag="ACHJ7J_004096"
     CDS             complement(9701..12145)
                     /gene="dmsA"
                     /locus_tag="ACHJ7J_004096"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO5002265.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMGHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
BASE COUNT         3209 a         3206 c         2903 g         2891 t
ORIGIN      
        1 gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat
       61 gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac
      121 ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat
      181 aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc
      241 tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc
      301 atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc
      361 ggagccgatc tctttacttc cacagtgttg attgttgttg ctaaggcgag tgggcgcatc
      421 acctggggtc agttggctaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca
      481 ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg
      541 ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc
      601 tgccttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc
      661 cgcagcctga tggacaaagc gtttatcatg gtgctgccgg tcgcgatgtt tgttgccagc
      721 ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac
      781 ttcgcatctc cggaattctg gaccgctgtc ggttctgcac cggaaaattt ttctcacctg
      841 accgtgatga acttcatcac tgataacctg attccggtta cgatcggtaa tattatcggc
      901 ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgatcaccat
      961 taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg
     1021 tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag
     1081 aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag
     1141 tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc
     1201 gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc
     1261 atcacctctc acgacgctgg ctacatcaac aaagcgttgg aaaaagttgt tggtctgcag
     1321 actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt
     1381 tcctgcaaag cgtacaaccg cgaactggac ccgatgatca aaaaaatctt cactgaatac
     1441 cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt
     1501 aaatccggtg ttctgaccgg tctgccagat gcttatggcc gtggccgtat catcggtgac
     1561 taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaata cgctcagttc
     1621 acctctctgc aggctgatct ggaaaacggc gtaaacctgg aacagactat ccgtctgcgc
     1681 gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac
     1741 ggctacgaca tctctggtcc ggcaaccaac gctcaggaag ctatccagtg gacttacttc
     1801 ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc
     1861 accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa
     1921 gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact
     1981 ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt
     2041 atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg
     2101 tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg
     2161 ctgaacttca agaaattcgc cgctaaagtg tccatcgaca catcttctct gcagtatgag
     2221 aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctatcgc ttgctgcgta
     2281 agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa
     2341 accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg
     2401 aagtctgaac cgatcaaagg cgatctcctg aactacgatg aagtgatgga gcgcatggat
     2461 cacttcatgg actggctggc taaacagtac atcactgcac tgaacatcat tcactacatg
     2521 cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc
     2581 accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa
     2641 tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa
     2701 ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc tgttgacctg
     2761 gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact
     2821 cagtctgttc tgaccatcac gtctaacgtt gtgtatggta agaaaactgg taacacccca
     2881 gacggtcgtc gtgctggcgc accgttcgga ccgggtgcta acccgatgca cggtcgtgac
     2941 cagaaaggtg ctgtagcgtc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa
     3001 gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt
     3061 cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa
     3121 ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa
     3181 aatccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac
     3241 tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa
     3301 ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg
     3361 ccttttttag cacgagagcc ttttttgtca gctatctata ctttaaggtg actgccaaaa
     3421 cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga
     3481 gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag
     3541 acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt
     3601 gtcataaccg cgacacctgg gacacgcatg gcggtaaaga agttaccgtt gaagatttga
     3661 tgaaggaagt ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat
     3721 ccggcggtga ggcaatcctg caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa
     3781 aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga
     3841 ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgatg
     3901 agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc gccaaatatc
     3961 tggcgaataa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg
     4021 atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggtaac gttgagaaaa
     4081 tcgaacttct cccctaccac gaactgggca aacacaaatg ggtggcgatg ggtgaagaat
     4141 acaaactcga cggtgttaaa ccaccgaaga aagaaaccat ggaacgcgtg aaaggcattc
     4201 ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca actttattga
     4261 gttgccgctt tgttattccg cgctgcataa ataattcaga gctctatttc taacgccctt
     4321 acagcacagc ttccaaatgt attacccaaa ccgccagtat atgtcctgcg gcttgcgcgc
     4381 atttctcgat tatgtaaaaa cgggtcagat ctgccagagt cagcgtcacc gaccacaata
     4441 attatttact attcgcttta cgcgccagca gcgggaatat cagtcctaat cctaccagca
     4501 caaacggcgt tgcaacattc agcgccaact ggaaggtcca ctctgcagtg aaggcttcca
     4561 ttttcgggaa gatccccgtc aaacaggcaa aggcagtaaa ggcaaaacac caaatcccga
     4621 cggtcattgc cagaggctta ttacgaataa agacatactc tggtgtatat ttctgcgcca
     4681 ggcgaacgac tgcaataaat gcaacaaata cccataaata acgcagcggc atcactaccg
     4741 agttaagatt caacagccat ttatagagat tgttcatatc accgatgccg agagtcggca
     4801 gcataatcag gatcgccacc agcaccaggg tcagaaaata gccattaacg ggcgtaccag
     4861 aagcgttggt acgacataaa ctggctggaa tatatttgct gtcagcatcg cctaatagca
     4921 ctttaagcgg ggcatcaatc gagaatacta gcgcagctac ctgtcccagg gtattcgcaa
     4981 tggcgtaaat caccattaaa gtattaccca tgttgtaata ctcgcccagc ttctgaaagg
     5041 cgtaatactg accgttagtc attaagtcat ccgggatatt acgcgagtca aacatcatgc
     5101 ccatcgccag tgagcccaga atggcacaaa ccgcaaccat caccgccagg catagcatcc
     5161 cttttggaaa ttcttttcct gggttgcgcg tttgattaac gtaaggagaa atcttctctg
     5221 ctccgccaac cgcgaaaacc agcattgaaa tagtggtaat gtaggtaaag tcgatatgag
     5281 gaatgaacgt ttcccaggta atgtttgtgg tcgcaatatg cacttcagta atcgcaggcg
     5341 cggttaccgc catcgccaca tacaggagcg acataacaaa catcgcaatc cctgccacag
     5401 aaccgacgat tttcagcgat ttcataccgc gtgaagcaac ccacataaag aagataaaca
     5461 gcaccagcga taacccctgt aacgctacga ctgaatattc tttgattagc gaaccgtcgc
     5521 ctttcatcgc ccaaccgagc gcaatcagta ttgcctgggg tttttgtgcc agatagggaa
     5581 tatgcaccac ccagtaggtc cacgcggcga ggtaagccag tccgggtccc atcgtgtgtt
     5641 taatccaagt actgaccccg cctttcccat ctttgaaggt cgagcctaac tgaccaacaa
     5701 ttagcgcata aggtgtgaaa tagagcgcaa agataaacac ccatgaaaaa acaaccacca
     5761 gcccctggtt ggcatagtta ttaacaacgt tgccaaaacc ccagacagtg ataaaagaca
     5821 tcagcgcaat gttgtaccat cgcagctgtt tttcctgaac attcccagcc atagacaaat
     5881 ccttcgccaa aattaaaggg gcatctgtaa aacgagggat tatgcgcggc aaataagaag
     5941 tgcggaaata gcgaaatgca aaattataac ctgctgcaca ttttattttt aaacgaataa
     6001 aatttggaaa aaagcaagtc agatagaaga aacaaaaaag cagagtgtga aacagtaagt
     6061 tcacactctg cttgaattca tttacacgtg agcaacgggt ttcggcgtat gaccggcgtt
     6121 gcgaagcagc atcagcaaat agataaacga tacgctggcg atcatgataa acaataaatt
     6181 atcggagaaa ttctgcatta gcatagcggt aaatgacggg ccaagcagac ttcccacggt
     6241 atagctcaac agtaaggcct ggttcatcgc caccagttga tgatgttcaa ctttctcgca
     6301 agcccaggcc atcgccaccg gatatagcgt aaagccagcg gcaccgagga tgaataacgc
     6361 tggggccatc gccgcctggc taagcatcgc gatactgccg agaatgacga caaagacctg
     6421 aacacgcaac accagcagtc gaccaaactt atccgccaga cgtccaatcg gccattgtcc
     6481 gaggataccc gcactgacca gtaccgccat ccagaaacca atgctggcat tgctcacccc
     6541 tttgtgattg aggtacaacg gcatcaggcc atatagagat cccagaacga ttcctgagat
     6601 aatgcagcca ttcacgccaa gccgcgcctg acggagtttt agcattgacg taattgacgt
     6661 cgaatcatgg ttttcagcct gctgattcag gacacgcgta aacaacagcg gtaagatccc
     6721 tgccaacgtc aaacctgtaa cccacggcaa tacgctcatc agctcggttg agactttgct
     6781 gaccagtaac tggcctaaaa acgttcccac gtaataaacc atcatatacg cagcaagcaa
     6841 acgcccacgg ttacgtgacg tcccactgca catcagcgcg ctctcaacga ctacccaaat
     6901 catggcacag ccgacgcccg cgacaaaacg ccacgccaac cagctccaga aaccaatcat
     6961 caggccaagg cccgcacagc cagcggcaaa aatgaaggag gccagataat agctgcggtt
     7021 aaagccaatg cgtttaatga cataccctgt cagcaatgta ccgacaaggt tgccggtaaa
     7081 ataggatgag ctgacaacgc ctacctgcca tgtggacatg tgttcctggg cgagccaaag
     7141 cggcacgagt gtatttaaca ccgcgatcgc cagagtcaac aaaagcaggc cagacagcaa
     7201 aagcatgaca ggccgggtat acgtggacat gaattaaaac cgtgaggaag ttcaaatttc
     7261 atgcgcatca tgccaccgcc aaaaacattg tcaatgggtg aaaatacgcg catatcgcaa
     7321 cggcggtatt atcgatttat attactcatt cataacgcta atactgaaga gtatgattaa
     7381 tctcatctga ttgtttttat tgatctaaca caaatttcat acgcttcgtt cagtcgaaaa
     7441 atttcatggt cacagcaaac tcatatttcc cgtctatgct ttcagagcaa tgcatcgcgt
     7501 ctgaccgatt tcagacgtac cactgactct cgaaaggaga agaggatgac caaaccgtat
     7561 gttcgtcttg ataaaaatga tgctgccgtt ttgcttgttg atcaccaggc tggtttactt
     7621 tcccttgtac gggatatcga acccgataag tttaaaaata acgtgctggc gctgggtgac
     7681 ctggctaagt atttcaactt accaactatt cttaccacta gttttgaaac cggtcctaac
     7741 ggcccattag ttccagaact gaaagcacaa ttccccgatg caccttacat tgctcgcccg
     7801 ggcaatatta acgcctggga taacgaagat tttgtaaaag cagtcaaagc gacaggtaaa
     7861 aaacagttaa ttattgccgg tgtggtaacc gaagtttgcg tggcattccc ggcgctttca
     7921 gccattgaag aagggtttga tgtcttcgtt gtgactgacg cgtcagggac ctttaatgaa
     7981 attacccggc attcggcatg ggatcgcatg tcgcaagcag gtgcgcaatt gatgacatgg
     8041 ttcggcgtgg cttgcgagct gcaccgtgac tggcgtaatg atattgaagg actggcgacg
     8101 ttgttctcta accatattcc tgattatcgt aatttgatga ccagttatga cacgttaacg
     8161 aagcagaaat aaaagagaac cggggccgaa accccggttg ttactgatta gcttgcgacg
     8221 gccatcccca cggtcatatg caaaccatag aatacgccgc gaccgattaa ttcccctgcc
     8281 agaagcagaa tgaatgagac agatagtagc ggaaccgcag gctgataacc ttttagctgt
     8341 ggtgcaatcc acaagcacag ggcaacagcc aaaagcacga tccgccagga catcagcgca
     8401 ccatagtccg gcaccagcgc tgcggcctgc tgcacagaac tatgaatagt cgccagctct
     8461 gcgccctgca tcactgacat cacgccactc actaccagtg caagtacaga aatggctggc
     8521 agcagacgca tcgcccagcc atcgacgcca gccagactca gcaacaggta acccagtaac
     8581 gggccgccca taaacattgt caggaagaag cccatcggcg tccagatgct gtaccaggtc
     8641 ggaacggtat caatgctgtt atagacacgc accatcatcc agacaaagac tacgccaaga
     8701 accatcgtca ctaccagcca cagcgtacgc aatgccggtg acagcttttt cagcattgcc
     8761 agcaaccagc cgatgccgcc tacggcaaaa aagatggaac cgctggcgat ttcgttactg
     8821 agtgctgaag cccctacccg gttgagcgag ttaaaggcgc gcatcggtga accaagatgg
     8881 agcatagagg cgataaagcc aatgcccatc agcacccata agccaaacat gcaggcgata
     8941 acacgctgct gggtttctgc tcgcaggtcg cctttgagca gcgccaaagc cagaacgata
     9001 aaaccacccg ccacacattg cccgaagacc gtgaagatca tcagcggcca ttcatgccat
     9061 ccacttccca tctcacacct ccttcgggtt tgccagatat ccagtggtat ccccggtcgg
     9121 gcggctattg gcattgggtt tgatcacaat attcggtttg gtaaagtgag ctcgcggcaa
     9181 cggcgcaacg gccgccagat cgccatgttt tttacgcagc tcgtcgatag ggccgaaatc
     9241 cagcgcccgc agcggacaag attcaacaca gatcggcttt ttaccctccg caacacggtc
     9301 ataacaacca tcgcatttgg tcatatggcc tttcgtttcg ttatattgcg gtgcgccata
     9361 cgggcaagcc atatggcagt agcggcaacc aatgcacaca tcttcatcga ccacaacaaa
     9421 accatcttca cgtttatgca tcgcaccgct cgggcagact ttagtacaag ccggatcttc
     9481 gcagtggttg cacgaaatcg acagatagta ggcaaacacg ttctggtgcc agacaccgtt
     9541 atcttcctgc cagtcgccac cagcatattc ataaatgcgg cggaagctga cttctggtgt
     9601 caaatctttg tagtctttac aagccagctc gcaggttttg caaccggtgc aacggctgga
     9661 atcaataaaa aatccatact gggttgtcat cggttactcc ttacaccttt tcaacctgaa
     9721 caaggtttgt atgtgacgga ttccccttag cgagaggaga cggacgttga gtggtcagta
     9781 cgttaataca accaccctta tcgacacgtt ttgcatccgg gtcataccag gcaccttcac
     9841 ccagtgcgac cacacccggc atcattcgtg gcgtcacttt cgcctcaata tgaacctcac
     9901 cacgatcgtt aaagatcctg actttatcgc cgttgtggat accgcgtttt tgggcatcaa
     9961 gcgggttgat ccacatttcc tgacggcaag ccgctttcag cacatcaacg ttgccgtaag
    10021 ttgagtgaac gcgagattta tagtggaaac ccgtaagctg cagcggatac tgtttgttca
    10081 gcggatcctg ataactttca aagcccggcg tgtagatcgg cagtggatcg atcacatcgc
    10141 cttctggcaa ttcccaggta gcggcaatgt cagccagcgc ctgcgaataa atctcaattt
    10201 tgcccgatgg cgtagtcagt gggtttgcct gcggatcttc acggaaggct ttataagcaa
    10261 cgtgatgccc ttgtgggtcg cgctttttaa agatcccctg cttgcggaac tcttcaaacg
    10321 ttggcagttc aggaatcgct tcccgcgact gggcatacag atgccccatc cactcttcct
    10381 gtgtacgacc ttcagtaaac tgttgctcaa cgccaagacg ttttgccagt tcgctggtca
    10441 tctcatagat ggtcttacat tcaaagcgcg gtttaatcac ctgatcgttg aaaatcacgt
    10501 aagacatatt cccgcaggat gcatccagcg caaagtccat ctgttcggaa gcggtgcagt
    10561 caggcagcag gatgtcagca tatttcgccg atgaagtcat gtggcagtcg ataaccacaa
    10621 tcagctcgca cttcttatca tcctggagga tttcatgggt acggttgatt tcagaatgct
    10681 ggttaatcag gcagttaccg gcatagttcc agatcatttt gatcggcaca tccagcttat
    10741 ctttcccgcg cacaccatca cgcagcgccg tcatttccgg gccacgttca atggcatcgg
    10801 tccacataaa catcgaaatg ctggtctgga tcgggttttc caaggtcggc atacggacaa
    10861 acggtaagct gtatgaacct tcacgcgcgc cgctgttccc tccattaata ccaacgttac
    10921 cggtcagaat cgccagcatc gagatagcgc gagtcgcgat ttcgccgttg gcgtggcgct
    10981 gcggccccca tccctggctg ataaacgccg gtttggtact gccgatttca cgagccagtt
    11041 tgatgatttt gtctgccgga acaccggtga tttgcgaggc ccattccggt gttttagcca
    11101 cgccatctgg cccttcaccc agaatataag ctttatagtg gccatttttc ggcgcactgg
    11161 ctggcagggt tttctcgtca tagccaacgc aatatttatc gaggaatgcc tgatccacca
    11221 ggttttcggt gatcatgaca tacgccagac cgttaaccag tgcggcatct gttcctggac
    11281 gaatagggat ccactcatct tcgcgcccgg caccggtgtc ggtatagcgc ggatcgatga
    11341 tgatcatgcg ggcattagat ttctggcgtg cctgttcaag atagtaagtc accccaccgc
    11401 cactcattcg cgtttcgcca gggttattac caaacagcac taccagctta ctgttttcga
    11461 tatccgacgg gctgttgcca tctgcccagc cgccgtaggt atagttcaga ccttccgcaa
    11521 tttgcgcgga agagtagtca ccgtaatggt tcagataacc gccgcagcag ttcatcagcc
    11581 gcgcgaccag ggtatttccc ggcggccagg agcgggtcat ggtgccaccc agcgtaccgg
    11641 tgccatagtt aagatagatg gattcgttgc cgtactcttt gatcaggcgc tgcatattgg
    11701 tcgcgatgat gtcgtaggct tcttcccagc taatgcgctc gaatttgcct tcaccgcgcg
    11761 ccccgactcg tttcatcgga tatttcaggc ggtccggatt atagacacgg cgacgcatgg
    11821 aacgcccacg caaacaggcg cgaacctggt gcaggccgtc gtaattgtca tcgccggtat
    11881 tgtccgtttc gacatatttg atttcaccgt ccacgacgtg catacgtagc gggcagcgac
    11941 taccacagtt aactgtacag gcgctccaga taaccttttc gtctgatttt gttggaatgg
    12001 cgctatcgac agcgtgcgca atccgactaa aaggtaatgt taatgcgctg ctggccattg
    12061 ccaggccgcc gatcgctgtc gtttttacca aaccacggcg actcacctca gcagccaata
    12121 ccgcatcagg gattttcgtt ttcataatgg ctcactcaag cttgctcacg attgattcca
    12181 gtaacaaacc gtattgtgta attatttct
//
LOCUS       DAZFFG010000127        12015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFFG010000127 DAZFFG010000000
VERSION     DAZFFG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.127"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            32..937
                     /locus_tag="ACHJ7J_004097"
     CDS             32..937
                     /locus_tag="ACHJ7J_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5002266.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(971..1573)
                     /gene="cobO"
                     /locus_tag="ACHJ7J_004098"
     CDS             complement(971..1573)
                     /gene="cobO"
                     /locus_tag="ACHJ7J_004098"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5002267.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEIWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1583..3235)
                     /locus_tag="ACHJ7J_004099"
     CDS             complement(1583..3235)
                     /locus_tag="ACHJ7J_004099"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5002268.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTSTATIIASKGQQIWTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKALL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMLDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3343..4623)
                     /locus_tag="ACHJ7J_004100"
     CDS             complement(3343..4623)
                     /locus_tag="ACHJ7J_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFO5002269.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTVSPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4784..5101)
                     /locus_tag="ACHJ7J_004101"
     CDS             complement(4784..5101)
                     /locus_tag="ACHJ7J_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO5002270.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5098..6468)
                     /locus_tag="ACHJ7J_004102"
     CDS             complement(5098..6468)
                     /locus_tag="ACHJ7J_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO5002271.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6472..7713)
                     /locus_tag="ACHJ7J_004103"
     CDS             complement(6472..7713)
                     /locus_tag="ACHJ7J_004103"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO5002272.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSINNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7713..9158)
                     /locus_tag="ACHJ7J_004104"
     CDS             complement(7713..9158)
                     /locus_tag="ACHJ7J_004104"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO5002273.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMSSGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9177..10565)
                     /gene="glmL"
                     /locus_tag="ACHJ7J_004105"
     CDS             complement(9177..10565)
                     /gene="glmL"
                     /locus_tag="ACHJ7J_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO5002274.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFARQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10565..11014)
                     /gene="glmS"
                     /locus_tag="ACHJ7J_004106"
     CDS             complement(10565..11014)
                     /gene="glmS"
                     /locus_tag="ACHJ7J_004106"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO5002275.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEEVCQLMAHDINQRHDVDTRILEEAI"
     gene            complement(11562..11984)
                     /locus_tag="ACHJ7J_004107"
     CDS             complement(11562..11984)
                     /locus_tag="ACHJ7J_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002276.1"
                     /translation="MIPSQISFNILPVNATYASETTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEISSDT
                     VIFSILSSLIDSAISKFQHQNPVNGSVNPEILYEDYTD"
BASE COUNT         2909 a         2929 c         2980 g         3197 t
ORIGIN      
        1 atggtttaaa ctatttttaa gggacggcat tatgcgaggg aaaataccta aaacggaact
       61 gctggtgact tttgaggtcg tcgcacgtca tgaaagttat accagagcgg cagaggagtt
      121 agcgttaacc caaagtgcag tattcagaca agtaagtgcg ctggaggaat ttttacgtac
      181 tccgttattt tcacactcta aaaaacgcat ttttcttaat gatgcaggga agtattacct
      241 ggggatagta aaagaaacat taaataagct ggaacgcgat accaatacca ttatgacctg
      301 gcagcctacg gttcaggtta ttgaacttgc cgttaatcct acttttagta cccactggtt
      361 gatcccaaat ttacatgaat tcacaaagtt acatcccgat attattgtta atattcattc
      421 tttagctaat aacggcgatt ttcttaatcg ggagtacgat gctgtcatta tgcgcgaaaa
      481 tttttgcgcg ccctgggcgg aagtagaata tctctttgat gaggagattt tgccggtatg
      541 cagcggcagt ttgcttgcaa tgtccgatca aaagctgtca gtggcagaac tgcttactga
      601 gttaccgctt ctgcatcaaa gcacccgtat tactggctgg gaagagtggt ttgctttatc
      661 tggcgttagc agtccgttgg tcaataacgg accacgtttc gatttgcttt caatgctgat
      721 tgccgccgtg cgctctaatc tcggcgtggc gctgttacca cggttcgcta ttcaacatga
      781 tttggatagt ggcgatatgg tgatcccctg tgatgtgcct attcgtaccg gtaatcgttt
      841 tattatgacg tggcaggaag agaagtctga ctcaccgcac ttgcagcaat ttcgtgaatg
      901 gttgctggcg aaatcggtcg taccgcagga gatgtaaaac ctgcgcctgg cgggatgcca
      961 ggcgcagtta ttaccagtcg atacccggct gggcctgtat gccgttatca aaagcatgtt
     1021 tgacggggcg gatttcgctt accgtatccg ccatttttaa tatctgactg tggcaaccgc
     1081 gtccggtgac aatgacactt tgttgtgccg ggcggttttg cagagaagcg atcacttctt
     1141 cggtatccag ataatggtaa gccagcatat aggtcagctc atcaagcacc actaagtcgt
     1201 aacgcttgtc agccagcatc cgtttgcttt ccgaccagat ctctttcgcc gcgtcgatat
     1261 ccgcttgccg gttctgagtc tcccaggtaa aaccggttcc cattatatga aactcaaccc
     1321 ccagcggctg gagaaggttg tattcgccgt tgtcccattg ccccttgatg tactgcgcaa
     1381 cccccaccgt tttgccatga cccaccgcgc gcgtcacagt accaaacgcg gcagtggatt
     1441 tccctttgcc gttgccggtg aaaacaatca agatcccttt tttctctatc gccgctgcga
     1501 cgcgcgtatc gacctgttcc ttcaatttct gttggcgctg cacatgccgt tcagttgaaa
     1561 ttcgcgcttc cattcatttt ccttaatgtc ccgcagggca actggcgcag cgatgttgct
     1621 gctcaaactg actaaagaaa ttattgcctt tatcatctac caggatgaat gcgggcagat
     1681 tttccacttc catcatccac accgcttcca tacccagctc cgggtattcc aggcaacgca
     1741 aacttttgac atattcttgc gccagtaacg cagctgcacc gccaatactt cccagattga
     1801 agccgccatg tttgtgacag gcatcggtga cctgctggct gcggttacct ttcgataaca
     1861 tcaccagact gccgccagct gcctggaagg tatcgacata accgtccatt cgaccgcccg
     1921 tggttggacc cagtgaaccg caggccatgt tctccggtgt tttcgccggg ccagcgtaat
     1981 agacaatatg atgcttcagg tactccggca tcggttcgcc gctatccagc cgcgctttta
     2041 ttttggcgtg ggcgatatcg cgcgccacta cgatgggacc acttaacgac acgcgggtgc
     2101 ctaccggtaa tctggcgaga tccagcatca cgtcacgcaa cggacggttt agatccagtt
     2161 gtacatgttg cgcgtgattt tcttcccgca gtgaggctgg aatatattgc cccggattgt
     2221 gttcgagttt ttccagccag ataccgtgtt tgttaatttt tgctttgata ttgcgatcgg
     2281 cagaacagga gagcgccata gctatcgggc aagagccacc gtgacgcggc agacgaatga
     2341 cgcgaatatc atgagcgaaa tatttccccc caaattgtgc accaatacca aattgctgac
     2401 tggcctcgag caacgctttt tccagctcaa tatcacggaa tgcttgtccc tgctcgtttc
     2461 cgctggtggg caaattgtcg taatatttcg ttgaggccag cttggcaatt tttaaggttt
     2521 gatcggctga aagccctccg acgacaaagg cgatgtggta aggcgggcag gcagcggtgc
     2581 ccagcgattt cattttttcg attaaaaatg cggtcagttt ttctggttgc aataaggatt
     2641 tggtttcctg ataaagcgcg gctttattgg cagagccgcc tcctttatta acacagagga
     2701 aatggtactc atcaccagcg acagcgctga tatcaatctg cgccgggaga ttggtttgcg
     2761 tattgacttc ggtatacata tccagcggcg cattttgtga aaagcgcaga ttattttcct
     2821 gaaaagtggt gtaaatgcct ttgcttaagg cttcggcgtc gttacctccg gtccatatct
     2881 gttgcccttt actggcaata atagttgcgg tgctggtatc ctgacagttg ggtaataccc
     2941 cttttgctga gacttcagca ttacgtaata actgcaacgc aacgtattta tcattactgc
     3001 tggcctgcgg atcgttaaga atgctggcga cctgttgcaa atgagcggaa cgtaagaaaa
     3061 aagaggcttc ataaaatgcc tgccgggcaa gcagcgttaa tgcttcagga gcgattttta
     3121 tcacttcttc gccgtctaat tccgttactg taatatgttg atcgctaatt aatgtatatt
     3181 ctgtaccgtc tttattttgt agaaacggtt cttgccagat aaatggtttg gacatatttc
     3241 acttttcacc ttaaacgtta gtgttactta attgccgtct aacttcttga tccggcttat
     3301 aaaaatgcgt agtcattggc ttctgattgc ttaagatccc cctcagggca gagcaacgtt
     3361 cgattcattg ggttttaatg ctttaccccg acagccctcg ccgaaatgtt cgccttccca
     3421 ccgactcacc atcgcagcgc ccattgcgtt gctcatgacg tttgtcgcgg aacgccccat
     3481 atcaaggaac ggatcaacgc ccatcagcaa gatcagcccc gcttccggaa tattgaactg
     3541 gttgagcgtg gcggcgataa ccaccattga ggcgcgtggt acgccagcca ttcctttcga
     3601 ggtcaacatc aggatcaaca gcatggtgat ttgctcaccg atggataaat ggatattgca
     3661 ggcctgggcg atgaaaactg tggcgaagga gcagtaggcc attgatccaa cgagattaaa
     3721 tgagtagcca atgggtaaga caaagctggc aattttgggg gaaacgccaa attgctccag
     3781 tttttcaagc gttcccggaa aagccgcttc agaactggat gtggtaaatg ccagcagggc
     3841 gggttccgaa agggcgcggg tcaggcgtct gatgcagggg ccgacataaa cgatggccag
     3901 accgataagc agcacccaaa gtaataacat ggtgaaataa aattcaccca taaagatccc
     3961 ggcgctcacc ataactgcca gtcctcgttc agcaatcaat gctgaaatag cggcgaatac
     4021 ggtcagggga gcgaagagca tgacgtagcc agtgagcttt aacatggcat gtgccagcga
     4081 atctaaggcg tgaacgatgg cgctgccttt ctcgccaatc gccgtcaggc tacagccgag
     4141 gaaaattgag aacaccacga tttgcaaaat ttcattgtgc gccatggcat cgacaatgct
     4201 ggtggggaaa gcatgcgaaa taaatacttt tagcgtaaag ggttctgacg cgaccactcc
     4261 tgtggtttcg gcaccgtgtg caacaaaatt aattcctgtg cctggcatga agaaatttac
     4321 cgttatcaag cctaaggcaa ttgacagcaa tgaggcgcaa ataaataaaa agagtgtttt
     4381 agaaaaaata cgaccaaggg ctttggcatc gcccatttta gcaataccta ccaccagggt
     4441 agagaccact aacggagcga taatcatttt tatcagtcgt aagaaaatcg tcgtgaatat
     4501 cgatatttct tgcgcataga gttttgctgt ttccggtgaa acagtgttat taagcactac
     4561 gccgataatc attccaagta ccagcgccaa aagaatcatc gtggttaaac ttattttctt
     4621 cattttttcc tccatcaggt gcacggcgat aaatcagaat caggagaagt agatgcctga
     4681 atagagattc aggcatctac ctgctattac aaccttacaa atagctgaag tgttgaactg
     4741 caataagtga cgggatgtca gttggctaaa agtattcgga tttttacaaa tcgaaactca
     4801 tatttaataa aggtgcatgt tgctgtgcac catagacatc attgtcacct acattcccgg
     4861 agataatatc tcgtggcatg acgattttta ctgcgttaga aggatcaaac cagacaatac
     4921 gatgaataaa gtcaggttca acatgatata agcgcgcaat cagttctggg gttaattgcc
     4981 ctgatgattt aactcgctca tagttttctt tcgttttaaa taaaatatct aaaaccagtt
     5041 cgtaaggtcc ggcatttttc gaacgaatga cttgcgccag tgagacaatt gattgtttca
     5101 tgcctgaact ccttctggcg tcacctgttc aatatggaaa tcaaaacgca gagcgtcgtt
     5161 ggcttcaatc aggtgatata tggaaaattc gtataccggc ccgccctgga tatcagaagg
     5221 ggagaacggg aacgccagat tacctgcggt agcgatgcgg ttttcatagc cgtagtgcag
     5281 catggtagag cgcaccagcg agcaaacgct gttggcaatt tcctgggtcg gtgcaactac
     5341 atcaagaaca atccccagct catgcccggc agtttgcatc ggttcatggt ctcccatcac
     5401 cccgtttttg ccgtacaggt ggaaattgat gcgaatgctg tcatcgtcga gcgacaggtt
     5461 acgcgaaacg ctggttttga cttcatcgat gattttatcg atcccggcga tcataatcgg
     5521 gtcgcgcgtt ccggcgatgg tcagacagcg gaagcccacc cgtcgtgcac cttccagttt
     5581 cagggcatac ggcgtttctt catgcttaga accgctgacg tagacttcgc cgtcattgac
     5641 tgctttaaat gtgcagcctt tcaggttcaa cacgccgcca ggtcctggca ggaagtaggg
     5701 atcggatttc tcatacagtg tgtgtgcagc tgctgacgtt tcggtaaatt tacgcttcgg
     5761 attaaatgtc ttcagcgtaa agccgttgtc atcaatgatg cccatcgcac agtcagagcc
     5821 tgagccgggc gttgcggcga ttgccgcgca ttcaaggatt ttcccacaat gcagcgccag
     5881 accttcatca aagccttgca tgatcgggag cgcagcaaag caggccgggt cgtaagcgcg
     5941 cccgcccagc accacttgcg cgccttcttt cagcgcccgc tggaagggtt cgatgcccat
     6001 ttgcgcgacg atatacgtac tttcgtcaat cgcgtcgtgg gttaacggcg ggacaaaatc
     6061 cagcgccgtg attttgccgt tatccagcgc ctggtggacg atggctttgt caacgtctgc
     6121 cgggatcaac gccatcgaga acgagaggtg ctcttcccgg gcaatctcat gaattatttc
     6181 tcggcaccac tccaggtgtg gtgcggcacc agaaccgcca gccgttccga tcaccaccgg
     6241 tatattattt tgcacgcctg cggtgatcat atagcgcaga tcgcgcttga cccctgcgcg
     6301 atcggtaaac ggtttgcctg ccccgaggta gtgggggcct ggatcggaag agcctgcatc
     6361 aacggcgatc agatcgggcg attctgccat cgctttgcgg aagctctctt ccgggaatcc
     6421 atacccgagg attgccgttg gcgataagac tttaaaagaa cgagccattt gttaatcctt
     6481 agcctgcaac agcgcaatag tacgattcat ttcgttgaag acgatatcga ggccttcgtc
     6541 aaagcccatc cctggtttta ccagcatacg catggggcga gcggcaaggg cgacgtggac
     6601 acaggtgcgg gcactgacat cagtttcatt gcaggtgccg ccctggtacg cttccatgct
     6661 gtggctattg cagtaaagaa ccgcatcgac gatgttgtta atactgccca gatccggggt
     6721 tttgatttgc accatgtgac aactggcggc atcagtgaaa tcaacaatat cctggtaggt
     6781 gttacaccat tcatcggcca caattttcac gccggaaccg aggcgcgtca gctctttggt
     6841 aatcgcggtc agcaggcgaa tttgatcggg cttgttaccg gcatcgaccg gcccttcaat
     6901 gtagagcggc aggccttgcg cttctttttc caggctggcg atgtattgcg cacagcgaag
     6961 cggatccata tcgaagatca gaccgatagt gccgtataca tcgatgtgca gggtagggtg
     7021 gtagcgtgcg ctggtgcgct tgcttagaat gcgatccgac aaccagcgga catattcgcg
     7081 cagtttttca cctttaaagc ccagcttctc ttcgacgtta ttaatcagcg catggggcag
     7141 cacgtcgatg cctttaagga tcatcttatc gacggcgata tatcgatcgt cgccgctctg
     7201 accaaataat ggaatggatt ccgccacgcg tggcaactgc cattcatcac agaccacttc
     7261 agttttcaga cggccggttg ccagcgcggt agcatcaagc agcgcctgtg ataatccgta
     7321 gcgcacggcg gtatgcagca agttgccgtc aatacgcaat ttgtcgaaga aacgggcatt
     7381 cggcaggaaa gcatccacat cgcggcctac cagtaatggc ttgatatggt cgttgaggaa
     7441 cggaataaag tgctctgcga ggaacagtgg atcgcgaccg cctgccccgg aatactgtac
     7501 ggcagtacag tcacctacgg cgaccgcgcc gttttccaga atcaactgta ccgatacgca
     7561 ctccccggcc tgacgaacgg cgttaaaccc ctgtgttacc ggctccccgg tataaaaaaa
     7621 gccgtcatga cccgctccgt tttttatcgc ctgctggtca tcgaaataga atgaggagta
     7681 gccagcggtg aacagggcct gttttatttt cattatggtc ttcctattaa tttactgtgg
     7741 gatacagcat tgatgtcatc tacaaccatc tggaatgtcg gttggcgtcc ttcacagcgt
     7801 gcgcgttccg caacgtaatc gtggtggagg tcgagaatat cttttggcaa tggaacggct
     7861 ccggcttcca gtacgcgtat cgcgccggtg ttgtcacgga ccggaagaat tttgcccgcg
     7921 ttgcaggcgg ctggcgcaaa cggcacatcc agtacgcccg cttcaaatgc cagcaccgta
     7981 ccgcgcgcaa tatcaccatt acccagttcg aacactttat tcagtacagc gcgaacctcg
     8041 ctcttaatca gttcaatttc cagttcaacc gcagggcacg gcgggaattt ctgctcattg
     8101 accatgttga gcatctggcg ggaggctttc agtccctgaa tgttggcagc ggcagtcggg
     8161 atgccccagg cttcgtgcgg acttttggtg atgactttgg tggcaccaga catccccgcg
     8221 acggctgcgc cccaggagat aatagcgaac gctttcgatt cgtcttccgg aaagccgccc
     8281 atccactggt gaaacacggt actcaattca tagtcggtat agccatagct ctgaaaatat
     8341 tcgtgtgcaa gttcacgcag agactggatc gccgcaacat cctgcgtcag gctgccgacc
     8401 tggccataac caacggtgat agatttcaca ccctgttcca gtgccagcaa tccttcgatt
     8461 atcgcgatag agtgcgagat aaacggcggg atcagcgtac ccgttagtgg gccaaacggt
     8521 tcgcggttga tgcggatccc gtgttcttcg tacatcccca ttaaacggtc gcagtactgc
     8581 cagtcgcgga tcgatttttc cagcgttaca cgtttggcat acggaatgtt gtaggaaatg
     8641 ccaccgcctt cataactggt aaagccgctg gacatcgaga tttcagccag cagacgcgca
     8701 tctggcgtgc cgtggcggat ctgtagcggt ttttgcaggg tttcggtgag gcgacggcaa
     8761 gctgcgacgc cgtggttaac taccggtaaa ccgttcagtt tagaggttcc ggcttcgatg
     8821 gattttttaa ttcctacagc cgcttcttcg taccgattca gacgggtata tgcatcgata
     8881 gtgctcggca gcagatcgca ctcttcttgt agtgttttca gtagttcgat gtgttcgtcc
     8941 atcagcgcca cacctgcgcg cggttggcta agggttttac cttccttgtc tgctttcaac
     9001 agcgcaagag agaagcgttt atgctcagga atggtttgtt ggtatttaac gccatcttca
     9061 aagttttcaa catccttgcc ggtttcccag gttttcagta cctgttgccg ctcggtcatg
     9121 aattcgtcat gggttaattt cttatttcga agttccattg attgcagcct taattgttat
     9181 agggttaagc attgaatact ggtgcgcgcg gcggccagcg gatcgactct ggcgacgttt
     9241 gccagcagtg gtaatagccc ccgggaatcg cggtaatatt caaattcggt cggtaagaga
     9301 atgcgtttac cgtcgtcgtt taactcgcgg tatttcaacc agtgatgcat atcgaactga
     9361 ctggcacgcg agagccatcc gccggatccg acgactttac gcacggttgt caggtcacgt
     9421 cccatctgta aatcgacatt gccgacgcag gtgcagacct cttttttggt gcccgcatgt
     9481 ctttcggtgg cataacccac acacagtccg gccaggacgg tatcgaaata tttctcttct
     9541 gcatttgcgg gtaagtagtc gggatgagcg actagatgac ggaggtaatg ataaaaagcg
     9601 tccagctgtg cgggttgccg agcaaaattc acattgacta actctttcgc actttcacca
     9661 accaccactg ccgagacgcg cattcccaga tcgccttcca ccgtccgttt tacgaagggt
     9721 tccggtacgc cgtgtaaaac ggtgtcgggc gagagcgtgt tggcacaggc ggaataaaca
     9781 tcggtggttg cgccgcccat gtcgatcagc atgaactctt tccagctgtg atcgacgtta
     9841 ctgattgcct tgaccagttc aaagaccgtc catggtgtcg gcattggttc ttcgcctgtt
     9901 ttatcaacaa tgacatccag ccctttccct ttaacgatgc gtttaaggaa tatgtcgcaa
     9961 atagcctggc gtgcggctaa agggttcgga tggtcaagat cgggcagaac gttgtcgacg
    10021 atggtcaggt ctttatgccc gaggatctcc tgcacctcgt cctgaatatc ccggtttccg
    10081 gcataaataa tggcgcagtc gagtttggat tctgccagca cacgggcgtt attcagtccg
    10141 taactttctt ctccgccgtc ggtgccgccg gtgaacaaga gaatatcggg ctgcgtttct
    10201 tccagtgcct ggatatcacg gcggttcagc ttgtaggcgt aatattgcgc aattttagca
    10261 cccgcagagt gagcggttac tttcgcggtt tccagggtga tagaaggcac caatcccatt
    10321 gcggcaaccg ccagcccacc tttggctgag gaggaatact tcaatgcaac ctcaccgctg
    10381 tttaacaggg ggagggcatt gtctacgttt aacacctggt tcaggctgga gaaaaacccc
    10441 ttcgccagat gatgggtggt ggttggggtc agtacgtggt taaccaacgt taacgcgtcc
    10501 ccctcccggg tgaagagggc tgctttggtc cacgtcgatc caatatcgac agagacgatt
    10561 tgcatcagat tgcctcttca agaatgcgag tatcaacgtc atgacgttgg ttgatatcgt
    10621 gggccatcag ctgacaaacc tcttctaagt catgacttgg ggcaaagacg cgatcaaatc
    10681 ccatctcttt gaacttggtt tccacgtcag cgaagtcatg ttttccgacc accagattac
    10741 ctcccacata gaggaggatg tcacccagtc cacgctcgat gcagcgttca cgcatgccca
    10801 gacagtcgat atcgccatgc ccatagatag aggagacgac aatcgcgtca gcgccagttt
    10861 caatagcagc atcaatatat tcgtcctggc tgaccattac gcccagatta atgactctga
    10921 agtcgtggtt gctaaaaacg cggtccagaa ctttattgcc tactgcatgg cagtccgcgc
    10981 caataacgcc aatcacaagt gttgctttct tcatgggtaa tcctttgtaa gacagagagt
    11041 aaaataagtt acacacgtcg tatttatggc cgttcataac ggcacaggtt taatatcaca
    11101 ggagtgtctt tatctgaccg taatgttgca attgatctat tttcaaaatg tctttcggga
    11161 aatgattgtt taaagtgtgc ttttaaattc ccatgttaat tcagtatgtt atgtcaacta
    11221 atgtcatgaa tgttaaattt ttaaaattaa cttgatttaa tgtattgaga taattgtgat
    11281 ctacttctaa tttgcatttt tgtgacgtgg ttttagccat gagtaaatgc atttcataag
    11341 atttaagtcg aatttcactg caattcatct ctgcttaatg taaaaataat gttgaaaata
    11401 acaaatggtt aatgattcat gcgaattatg accttcgtta gctcttttga tttttcgtaa
    11461 aaaagagagg tacatgaagg tgctaccagt atgagtaacg ttaatcaaag gttttattac
    11521 gtctggcaat aacgcaggcc atttttcgcg acagggtgga tttaatcagt ataatcctca
    11581 tacaatattt caggattaac tgaaccgtta accgggtttt ggtgttgaaa tttagaaatg
    11641 gcggagtcaa tcaatgaaga cagtatcgaa aaaataactg tgtcagaact aatttcaatt
    11701 aatccttctc gttgctgacc aaaaaagata tttatcttga tttcatttgt actacggcaa
    11761 tcaatatgca gtaatttttg atcgctgaga ttttctgttt cataaatgtt tttggcgata
    11821 gtcggatgta ttgatgcgag acatctgctc agataagcca gatcctttag ggaacacccc
    11881 gctgagtaga ggatgtcaga gaatttttgt gtgtcaactg ttgtctcaga agcgtatgtt
    11941 gcattgactg gaaggatatt gaatgagatc tgagatggta tcattattta aatctttccg
    12001 tgagtaataa aaatt
//
LOCUS       DAZFFG010000128        11905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFFG010000128 DAZFFG010000000
VERSION     DAZFFG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.128"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            8..610
                     /gene="yebB"
                     /locus_tag="ACHJ7J_004108"
     CDS             8..610
                     /gene="yebB"
                     /locus_tag="ACHJ7J_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO5002277.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(612..1133)
                     /gene="ruvC"
                     /locus_tag="ACHJ7J_004109"
     CDS             complement(612..1133)
                     /gene="ruvC"
                     /locus_tag="ACHJ7J_004109"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO5002278.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1168..1908)
                     /gene="yebC"
                     /locus_tag="ACHJ7J_004110"
     CDS             complement(1168..1908)
                     /gene="yebC"
                     /locus_tag="ACHJ7J_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5002279.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEEAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1937..2389)
                     /gene="nudB"
                     /locus_tag="ACHJ7J_004111"
     CDS             complement(1937..2389)
                     /gene="nudB"
                     /locus_tag="ACHJ7J_004111"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO5002280.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2507..4279)
                     /gene="aspS"
                     /locus_tag="ACHJ7J_004112"
     CDS             complement(2507..4279)
                     /gene="aspS"
                     /locus_tag="ACHJ7J_004112"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO5002281.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4589..5155
                     /gene="yecD"
                     /locus_tag="ACHJ7J_004113"
     CDS             4589..5155
                     /gene="yecD"
                     /locus_tag="ACHJ7J_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5002282.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAR
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5152..5970
                     /gene="yecE"
                     /locus_tag="ACHJ7J_004114"
     CDS             5152..5970
                     /gene="yecE"
                     /locus_tag="ACHJ7J_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO5002283.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKMLPEIGAVPAIPQQSSLF"
     gene            6023..6418
                     /gene="yecN"
                     /locus_tag="ACHJ7J_004115"
     CDS             6023..6418
                     /gene="yecN"
                     /locus_tag="ACHJ7J_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO5002284.1"
                     /translation="MVSALYAVLGALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6459..7202
                     /gene="cmoA"
                     /locus_tag="ACHJ7J_004116"
     CDS             6459..7202
                     /gene="cmoA"
                     /locus_tag="ACHJ7J_004116"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO5002285.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7199..8170
                     /gene="cmoB"
                     /locus_tag="ACHJ7J_004117"
     CDS             7199..8170
                     /gene="cmoB"
                     /locus_tag="ACHJ7J_004117"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO5002286.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8335..10764)
                     /gene="torZ"
                     /locus_tag="ACHJ7J_004118"
     CDS             complement(8335..10764)
                     /gene="torZ"
                     /locus_tag="ACHJ7J_004118"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO5002287.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10789..11889)
                     /gene="torY"
                     /locus_tag="ACHJ7J_004119"
     CDS             complement(10789..11889)
                     /gene="torY"
                     /locus_tag="ACHJ7J_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO5002288.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPNVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2696 a         3170 c         2927 g         3112 t
ORIGIN      
        1 tgacaacgtg aatattaatt accctgctga atatgaaatt ggagatatcg tctttacatg
       61 tataggtgct gccttatttg gtcaaatatc agctgcatca aattgctgga gtaatcacgt
      121 cgggatcatt atcggtcata acggtgaaga cttcctggtt gcagaaagcc gggttcccct
      181 ttcaaccatc accacgctat ctcgttttat taaacgttcg tctaatcaac gctatgctat
      241 aaagcgatta gacgctgggc tgacagaaca acaaaaacaa cgaattgttg aacaggttcc
      301 ctcccggcta cgcaaacttt accacaccgg ttttaaatac gaatcttcgc gccagttctg
      361 ttcaaaattt gtttttgata tttataaaga agcgctatgt attccggtgg gtgaaataga
      421 gacgtttgga gaattgttaa atagcaatcc gaatgcaaaa ctcactttct ggaaattctg
      481 gttcctgggt tctattccgt gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca
      541 tccgggtttg gtgttgattc atgcggaggg agtggaaacg cctcagccgg aactgaccga
      601 ggcggtataa cttaacgcag tcgccctctc gccaggttca gccgcgattc gctcatctgc
      661 atcgcattct gactaacgtg gcagtgggtg atggcaatcg ccagcgcatc ggcggcatcc
      721 gcctgtggat tagcgggcag tttcagcaag gtgcggacca tatgctgcac ctggcttttt
      781 tcggcactac caatacctac caccgtttgc tttacctgac gtgccgcgta ttcaaatact
      841 ggcaactcct gattcaccgc cgccacaatc gccacgccgc gcgcctggcc cagtttcagg
      901 gctgagtcag cgttctttgc cataaagact tgttcaatgg cgaaataatc aggctggaac
      961 tgggtgatga tttccgtcac gcccgcatag atgagtttca gacgagacgg taaatcatcc
     1021 actttggtgc ggatgcatcc gctacccagg taggacagtt gcctacctac ctggcggatg
     1081 acgccgtagc cggtcacgcg cgaacccgga tcaatgccga gaataatagc catcacgcgt
     1141 ctccgttttg ctgtttagca ggcctcatca gagagtcgct gcgacctcat cagagatttc
     1201 accgttatgg taaacttcct gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat
     1261 cagtttcggt gcggtttctg catccatatc agctttggta gacgggatca tggaaacttc
     1321 cgcgctgtct gctttcagac ctgcctcttc cagtgcgtcg cgcactttac ccatttcttc
     1381 ccatgcagtg tagacatcaa tcgcgccgtc atcataggtc acaacgtctt cagcacctgc
     1441 ttccagtgct gcttccatga tggtgtcttc atcgcctttc tcgaaggaga tcacgccttt
     1501 tttgctgaac agataggcta cggaaccatc agtaccgagg ttaccgccac atttgctgaa
     1561 tgcatgacgc acttcagcaa cggtacggtt gcggttgtca gacagacatt caatcatgat
     1621 tgccgtgccg ccaggaccgt aaccttcgta gatgatggtt tccatgtttg catcatcatc
     1681 accgcccaca ccacgtgcaa ttgcgcggtt cagtgtgtcg cgggtcatgt tgttagacag
     1741 tgctttatca attgctgcac gcagacgcgg gttagcgtcc ggatcgccac cgcccagctt
     1801 agccgcggtt accagctcac gaatgatttt agtgaagatt ttaccgcgct tagcatcctg
     1861 cgcagcttta cgatgtctgg tgttggccca tttactatga cctgccataa aaatatctcc
     1921 agatagccct gcctgttcag gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc
     1981 tccaggactt agtgagcgcc gccgcagcag gcgcatcaag ccacttgtaa gccagatgtt
     2041 cagtgaaaac gatctgccgc tcgtgcggaa gcgcaagaca gaaccaggat tccgtattac
     2101 gcgtcacgcc cggcgcatag cgatgacgta aatgtgaaaa aatttcaaac tcaaccgtgc
     2161 gctgacagtc aattaaggtc agttgttcag cgacaacatc aatggtgacc tcttccttta
     2221 cttcgcgcat ggcagcttgc ggcgcggttt caccctcttc cacgctgccg gttaccgact
     2281 gccagaaatc gggatcgtca cgccgctgca acatcagcac ccgtttcgta tcttgtgcgt
     2341 agatgaccac taagatcgaa acgggacgct tatacacttt atccttcacg ctgcctcttc
     2401 gttgactgcc ttcgctcatc ccattcacat agttatctat gctcatggga gttcactcag
     2461 ttgccgcctc gatgcaacgc gaatgatttc gtgtatttga gtcatatcag ttattctcag
     2521 ccttcttcac aacctgaatg ctcagctcag ccagtgcagc cgggttagca aagctcggtg
     2581 cttcagtcat cagacacgct gccgccgtgg ttttcgggaa ggcgataacg tcacggatat
     2641 tgtcggtgcc ggtcagcagc atggtcagac ggtcaagacc gaatgccaga cctgcgtgcg
     2701 gcggagtacc gtatttcaga gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt
     2761 cgttgatacc cagaatacca aacaccgtct gctgcatatc accattatgg atacgtactg
     2821 aaccaccgcc cacttcgtaa ccattgatga ccatatcgta agcgttcgcc accgcatttt
     2881 ccggtgcagc tttcagctct gcagccgtca tgtctttcgg tgaggtgaac ggatggtgca
     2941 ttgccgtcag gccgccttca ccgtcgtctt caaacatcgg gaagtcgata acccacagcg
     3001 gtgcccattt gctttcgtcg gtcagaccaa ggtctttacc cactttcagg cgcagtgcgc
     3061 ccatcgcgtc ggcaacaatt ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt
     3121 gcgcggcagt acgatccagg atggcttcga tgatttctgc attaaggaac ttcgctaccg
     3181 ggctgttgat accttccaga cctttcgcgc gttcgttaac tttgatgtaa gccagacctt
     3241 tcgcgccgta gattttaacg aagttaccgt attcgtcgat ctgcttacgg gtcagcgatg
     3301 cgccacccgg aacacgcaga gcggcaacgc gacctttcgg atcgttcgcc ggacctgcaa
     3361 atactgcaaa ctcaacagat ttcagcagat cagcaacgtc ggtcagttcc atcgggttac
     3421 gcagatccgg tttatcagaa ccataacggc gttcggcttc cgcaaaggtc attaccggga
     3481 aatcgcccag atccacgccc ttcacttcca gccacagatg gcgcaccagc gcttccatca
     3541 cttcacgcac ttgcggcgcg gtcatgaaag aagtttccac atcgatctga gtaaattcag
     3601 gctgacgatc agcgcgcagg tcttcgtcac ggaagcattt aacgatctga tagtagcggt
     3661 caaagccgga catcatcagc agctgtttga acaactgtgg ggattgcggc agtgcgtaga
     3721 atttaccttt gtgcacacga gaaggcacca ggtaatcacg cgcgccttcc ggcgtggctt
     3781 tggtcagcat cggagtttcg atgtcgagga agccgtggtc atccataaaa cggcgcacca
     3841 ggctggtgat tttagcgcgg gttttcaggc gctgagccat ttccggacga cgcaggtcga
     3901 ggtagcggta tttcagacgc gcttcttcgg tgttgacgtg gttagagtca agcggcagaa
     3961 catctgcgcg gttgatgata gtcagcgagg acgccagcac ttcgatttcg ccggtcgcca
     4021 tatcgcggtt aatatttttt tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga
     4081 actcattacg cagttcagag gccagcttta acgcgtccgc acgatccgga tcgaaaaata
     4141 cctgcacgat accttcgcgg tcgcgcatat cgatgaagat caggctacca agatcacgac
     4201 gacggttgac ccaaccacac agagtcacct gctgccccac gtgggacaaa cggagctgtc
     4261 cacaatattc tgtacgcatg agatatccct taacttagct gccggcggat gccccctgct
     4321 gcgcaggtga ccaagtcgca gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct
     4381 attatactgg aaattctgcc gcaccgtaag agcctggccc gcgctggaac gcctcgttac
     4441 cactttatat cgggcctgaa atcagactct acgccagttt gctataaagg tgttgcccga
     4501 actcataaaa attaacaaaa tttgtcgttc cgccatcggc taatcgcatt aaggtgtgag
     4561 gcacgatttt gttttttcag gagtcatcat gcttgaactt aatgctaaaa ccactgcgct
     4621 ggtggtgatc gatttacaag aaggcatctt accttttgcc ggtggtccac atactgccga
     4681 tgaggtagtt aatcgcgccg ggaagctggc ggcgaaattt cgcgccagcg gtcagcccgt
     4741 gtttctggtg cgcgttggct ggtctgccga ttacgccgaa gcattaaaac agccggttga
     4801 tgccccttcc cccgctaaag tgttgcccga aaactggtgg caacatccag ctgcattagg
     4861 tgcaactgac agtgatatcg aaataatcaa acgtcagtgg ggtgcgtttt acggtacgga
     4921 tctggagttg caattacgcc gccgaggtat cgatacaata gtgttatgtg ggatctcgac
     4981 caatatcgga gttgaatcca ccgcccgcaa tgcctgggaa cttggcttta atctggtgat
     5041 tgccgaagat gcctgtagtg ccgctcgcgc cgagcagcac aataacagta tcaaccatat
     5101 ctacccgcgc atcgcccgtg tgcgtagcgt ggaagagatc ctcaacgcgt tatgatttac
     5161 atcggtctac cgcaatggtc gcatcctaaa tgggtgcggt tggggatcac cagccttgaa
     5221 gagtatgccc gccactttaa ctgcgtggag ggcaacacca cgctttacgc cctgccgaaa
     5281 cccgaggttg tcctgcgctg gcgtgaacag accacagatg acttccgctt ctgttttaag
     5341 tttccggcga ccatttcgca tcaggcagca ttacgacatt gcgatgattt agtgactgaa
     5401 tttttgaccc gcatgtcacc gttggctccg cgcattgggc aatactggct gcaactgcct
     5461 gccacattcg gcccacggga gctgcctgcg ctttggcatt ttctcgattc tctccctggc
     5521 gaatttaatt atggtgtgga agtccgccac ccacagtttt tcgccaaagg ggaagaggaa
     5581 caaacgctta atcgcggttt acatcagcgc ggcgttaatc gggtgatttt agacagccgc
     5641 ccggttcatg cagcacgtcc acacagtgaa gctattcgcg acgctcaacg aaaaaaacct
     5701 aaagttccgg tacatgctgt actgacggcg acaaatccgc tgatccgttt tatcggtagt
     5761 gatgatatga cgcaaaaccg ggaattattt caggtctggt tacaaaaatt agcgcagtgg
     5821 catcagacca ctacgcctta tcttttttta catacgccag atattgcaca ggccccggaa
     5881 ctggtacata ccctgtggga agacttacgt aaaatgcttc cagagatcgg agcagttccg
     5941 gctattccac agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc
     6001 ggccatttta aagggagttt gtatggtaag cgcgctgtat gccgttttag gtgcgttgtt
     6061 attaatgaag ttctcttttg atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg
     6121 tgacggtggt tttagcgaac tgcaaagcgc tattcgcatt catggtaacg cggtggaata
     6181 tattcccatc gcaattgtgc tgatgctgtt tatggaaatg aatggcgcag aaacctggat
     6241 ggtgcatatt tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca
     6301 tcaccgtctg tttcgctggc gacgttccgg catgagcgcc acctggtgtg cgctgttgct
     6361 gatggtgctg gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg
     6421 cacaatacgc cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat
     6481 tttctgcccc tatcgccaga ctgggcgact ggacctttga tgaacgggta gctgaagtct
     6541 tcccggatat gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta
     6601 tgttagccga gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg
     6661 gcgcggcgac gctctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca
     6721 tcgacaactc cccggcgatg attgaacgct gccgtcgtca tattgacgcc tataaagccc
     6781 ctacgccagt agacgtcatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga
     6841 tggtggtgct gaattttacc ctgcaattcc tggaaccttc cgagcgccag gcattactgg
     6901 ataaaattta tcaaggcctg aacccgggcg gcgcgctggt gctttcggaa aaattcagtt
     6961 tcgaagatgc caaagttggt gaactgttgt tcaacatgca ccacgacttt aaacgtgcca
     7021 acggttacag cgaactggag atcagccaga agcgcagcat gctggaaaac gtgatgctga
     7081 ccgattccgt ggaaacccat aaagcacgcc tgcataaagc cggttttgag catagcgagc
     7141 tgtggttcca gtgctttaac tttggttcac tggtggcatt aaaagcagag gacgctgcat
     7201 gatcgacttt ggtaactttt attctctgat tgccaaaaat catctttcac actggctcga
     7261 aacgctgccc gcgcagattg ctaactggca gcgcgagcag cagcacgggt tgtttaagca
     7321 gtggtcaaat gcggtggagt ttctacctga aattaaaccg tatcgtcttg atttattgca
     7381 tagcgttacg gcagaaagcg aagagccact gagcgcaggg caaattaaac gcattgaaac
     7441 gctgatgcgc aacctgatgc cgtggcgcaa agggccgttc tcactgtatg gcgtcaacat
     7501 cgataccgaa tggcgttccg actggaaatg ggatcgtgtt ctgccccatc tttctgattt
     7561 aaccggacgc accattctgg atgtcggctg cggcagcggt tatcacatgt ggcgcatgat
     7621 tggcgcaggc gcgcacctcg cggtggggat cgaccctacg caactgtttc tgtgccagtt
     7681 tgaagcggtg cgtaaactac tgggtaatga tcagcgcgcg catttgttac cgttaggtat
     7741 tgaacaactt ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttatca
     7801 tcgtcgttca ccgctggagc atctctggca gttgaaagat caactggtca atgaaggtga
     7861 actggtgctt gaaacgctgg ttattgatgg tgatgagaac acggtgctgg tgccgggcga
     7921 tcgttacgcg caaatgcgta atgtctattt catcccttcc gcgctggcgc tgaaaaactg
     7981 gctgaagaag tgtggttttg tagatatccg cattgcggat gtgagcgtta ccaccacaga
     8041 agagcagcga cgcaccgaat ggatggttac cgaatcgctg gccgatttcc tcgacccaca
     8101 tgatccgggt aaaacggtgg aaggttatcc ggcacctaaa cgcgcggtgc tgattgcgcg
     8161 caagccgtaa aggtctggta ttactgccgg atgcggcgtg aacgccttat ccggcctaca
     8221 aaaccttgct aattcaatat attgcaggga ctatgtaggc ctgataagca tagcgcatca
     8281 ggcagcttta tgtttgcata acctcagcgc ccgtttccgg gcgcttttca cgtcttacgc
     8341 ctgaacagct ggctgatcaa acgccgttaa cttcggtgcg ttacccgtat atttttcgat
     8401 atacaccagc gcagagttac cggcacaggc atttgccagc tgcgagctgg ggatatccgc
     8461 cgttaacacg ttcgcactgc cgtttttaca caagccattt tccagatctg gccatgcacc
     8521 ttcatgcacg cataccacgc cttttttgat cccgtcagtc accacagcgc ctgtcaggat
     8581 ctgacctcgt ttattccaga cacggaccag atcgccattc gcaataccaa agcgagcagc
     8641 atcttcggtg tgaatagtga ttggttcacg atcggcaacc gcatattttt tacgcagttc
     8701 cgcatagtta agctgactgt gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg
     8761 cttctcgtcg gcggtaccct tccactcatc aggcgcaagc caggttgggt gtgccgggca
     8821 atccttatag ccaaattttt ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt
     8881 acccagcgca tttttcactg gatcggcgcg gaaatcacca taacgaacat actgttcgtt
     8941 cttctcgctg cggcgcattt cgatcagttt attttgctgc cagaaggcat taaacattgg
     9001 catagtgacg cgttgcgcac gggcaccttt ctgagcggca tcatagaaaa atttcagcca
     9061 cgccatttca tctttacctt cggtatagat ctcttttccg ccaggtttca gtaattcagc
     9121 aagatcggca aacacgtcaa aatcgttacg cgcttcaaat tgcggagcga cagcctgctt
     9181 catcggtaca atatgctggt tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa
     9241 cgatgtggtg atcggtaata cgatatctgc atgtttagca gctgcggtcc agtagcattc
     9301 agaaacgacg atcatctccg gtttctgcca tgctttgatc agacgattag tatcctggtg
     9361 atgggtaaag ttaccgccac ccgcccacca gatcattttg atattcggat acgtttgttc
     9421 tttaccgtta tgctgatatt tgccgccagg attttccagc gcatcgacga tacgggcaac
     9481 aggaatcgcc gtcattccgc catcatcagc ggcctcgctg gcctgtccgg caatcgccgc
     9541 agacatttcc ggcagcacgc cgccaacacg cgtcgggttg ccaccattgg agtaatgata
     9601 agagaaacca aaaccgccgc ccggtgtacc aatctgccct aacatcgccg ccagcgtcac
     9661 cagcatccag tgtttctgtt caccatattg ctggcgctga attccccagc ctgccatcag
     9721 cattgtgcga ttcgccgcca tcagttcagc gagtttgact atctgcgctt ccggtacgcc
     9781 agtaatttcc gccgcccagg ccgcgctctt cggagtatta tcgctcttac ctgtcagata
     9841 ctcttcaaac tgcggatacc cggtggtgta tttttcaagg aacactttat cgtgtttgcc
     9901 ttgtgtcatc agggtatgcg caatccctaa catcagtgcc acgtcggtgc ccatattcgg
     9961 cgcgatccag gtggcattat cgccaaagaa ctcgatggtt tcggagcgga taggatcaat
    10021 ggcaatcact ggtttgccag attttttcag ctgatggaag tattccagcc cttgctcatc
    10081 ggtactgctc caggcaattt ttaaggtatt cagcgggttc attccccaca gcaccacaac
    10141 ctggctgttt tccagaatca gcggccagga ggtctgctgt tcatacacct ctacagaacc
    10201 gaccacatgc ggcatgatca cctgtgctgc cccggttgaa taatcgccgc tatgcccgga
    10261 ataaccgccc gccaggttca tataacgttg cagtaaggtt tgcgctttat gcaacacgcc
    10321 agaagagcgc cagccgtaag atccggcaaa aatggccgat ggtccgttag ctttacgaat
    10381 acgctcatgt tgctcatgaa tcagttttaa tgcctgttcc cagctcacct gtacataggt
    10441 atcttcgcca cgacctttcg ccggttgcag tggattgtcg agatagcttt ttctcaccat
    10501 cggatgctga atacgcgccg tggtgtgtac ctgatccgcc gccgtagact gtaaggagtt
    10561 cggtatggtt ttcgccagcg cgcctgtcga agaaacaatc ttgccgtcct tcacttctac
    10621 gttcatcgct ccccaacgtc ccgcggtgag gattttaccg cccttctctt ctgcccatgc
    10681 gggtagcggt gctgccgatg tcaccaccag cgctccagcg gcaataccgc tgtgtttaat
    10741 aaattcacgt cttgttaatg tcataacttc ctccctgatc aacgaggatc actgtttctc
    10801 ggtaatatct ttggcgttgt actggaaata ccgcgttaaa atgtccagtt cgttttcgct
    10861 catgctggtt cgtgccccca ttcctttggc aatggacggc cacgcattga cggtgtaatg
    10921 gtcggcggca ataggggcat gacaaccagc gcaataggta tcgtcaagtt tttcagcgta
    10981 ttgccatagc ggtttacggt ccgctaatgc gggatcggta agcgcaccct gtaaagacgc
    11041 ctgacgccat tgattgccgt attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt
    11101 gataccttct tcacttaatg tggctagcgc cagccgttga cctgccgcca ggtagagcgt
    11161 gttttcactg ccctgcattt gataaccctg taacagaacg ttcggctgtt tgccactggc
    11221 atcaacgacg gtgagatcgg taccaggatt cacggtagcc agctcgccta tgcgagaagt
    11281 tttgaaagga taaatatgtg cgccattagt cactgaagta gcggcctgac tttccagctc
    11341 atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc ctttatgaca
    11401 atcgatacag gtttcgccgc ccttttgtgc tttgttatgc attttctgcg cagattcact
    11461 ttgcgaggca atatccatgg catcaaaaga atggcaacta cggcacgttg cagagtcagt
    11521 tgcttttaat tctttccata ctgtttcggc catttcctgg cgatgagctt cgaacttatc
    11581 gtcactgtct attttgccgc taacaaattc atgataaata tctttagatg cttttaattt
    11641 agcgaataaa taatccatcc ctgactttgg aatatggcaa tcggcacatt ccgcacgtat
    11701 ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt tactcatcga
    11761 gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt aaggcttttt gcgccagcaa
    11821 gaacccgccg ccaccaacca caaccgctat cagcaaaaac aataacccaa tgcgtttttt
    11881 ccctcgcata gattcctcta attaa
//
LOCUS       DAZFFG010000129        11863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFFG010000129 DAZFFG010000000
VERSION     DAZFFG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.129"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            78..680
                     /gene="lpfA2"
                     /locus_tag="ACHJ7J_004120"
     CDS             78..680
                     /gene="lpfA2"
                     /locus_tag="ACHJ7J_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFO5002289.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            789..1469
                     /locus_tag="ACHJ7J_004121"
     CDS             789..1469
                     /locus_tag="ACHJ7J_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5002290.1"
                     /translation="MYLLRLFFAVTLITQMSMAMAGIVVGGTRVIFYGDKPDTTISIF
                     NKETNLPYLLQTWVDPFNKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRE
                     SVFWLNVKNIPPSIDKKNTNVLEIAIKTRIKLFWRPAAVKIIPEQAIKNVKWSRQGNN
                     LIVENPNPIHINVMSVTVDDKDVSLNMIRPFETLTLPIPAGVAGKSLVWRFINDYGAI
                     SQPLKKNL"
     gene            1491..4025
                     /locus_tag="ACHJ7J_004122"
     CDS             1491..4025
                     /locus_tag="ACHJ7J_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5002291.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFVQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYGDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSTEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSSSNHHDDNNDNFGKEQIF
                     MFSMSIPLSGWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            4037..5128
                     /locus_tag="ACHJ7J_004123"
     CDS             4037..5128
                     /locus_tag="ACHJ7J_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002292.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGVCFNSDGATHMSNLSFGPLT
                     VAAANNHSGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLE
                     RTLNGLNYYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNL
                     DSGRSAKLSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQN
                     CELTPNQSIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASM
                     IAANRSADATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFY
                     SAPASLTGAKPAPDNGFTATATLVIEFTN"
     gene            5156..6238
                     /locus_tag="ACHJ7J_004124"
     CDS             5156..6238
                     /locus_tag="ACHJ7J_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002293.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGDERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            6450..7490
                     /gene="pstS"
                     /locus_tag="ACHJ7J_004125"
     CDS             6450..7490
                     /gene="pstS"
                     /locus_tag="ACHJ7J_004125"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO5002294.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLTQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            7577..8536
                     /gene="pstC"
                     /locus_tag="ACHJ7J_004126"
     CDS             7577..8536
                     /gene="pstC"
                     /locus_tag="ACHJ7J_004126"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO5002295.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSLIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            8536..9426
                     /gene="pstA"
                     /locus_tag="ACHJ7J_004127"
     CDS             8536..9426
                     /gene="pstA"
                     /locus_tag="ACHJ7J_004127"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO5002296.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVLGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            9517..10290
                     /gene="pstB"
                     /locus_tag="ACHJ7J_004128"
     CDS             9517..10290
                     /gene="pstB"
                     /locus_tag="ACHJ7J_004128"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO5002297.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            10305..11030
                     /gene="phoU"
                     /locus_tag="ACHJ7J_004129"
     CDS             10305..11030
                     /gene="phoU"
                     /locus_tag="ACHJ7J_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO5002298.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            11455..11590
                     /locus_tag="ACHJ7J_004130"
                     /pseudo
     CDS             11455..11590
                     /locus_tag="ACHJ7J_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11557..>11863
                     /locus_tag="ACHJ7J_004131"
     CDS             11557..>11863
                     /locus_tag="ACHJ7J_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO5002299.1"
                     /translation="MSVGNKEKYSITHKDITNLIAGDRQSFLKLTLWEFILDLFPMYH
                     IKEAKETIVEFVTKTEEKVKRFERIKSLAIPEEKWRFSTTTEFTVDENKDIIVSRVFK
                     "
BASE COUNT         3146 a         2712 c         2923 g         3082 t
ORIGIN      
        1 aaataccgat cgggtttatc caaatttaat aaatggataa cacacaggaa taatatcaaa
       61 ttaatggagt tatatttatg aaaccaaata tgattgtagg agcattagcg ttaacttctg
      121 tgtttatggc aggtcaccta caggcggctg atggaacagt ccatttccgt ggtgaaatta
      181 ttgacagtac ttgcgaagtc acacctgaaa ctaaagatca ggtcgttgac ttaggcaaag
      241 taaaccgtac agcctttagt ggcgtcgatg atgtggctgc cccgacggct ttttctatcg
      301 atctgactca atgcccggaa acctttaagt ccgccgcaat tcgtttcgat ggtaatgaag
      361 atgctcatgg taatggcaac ctggcaatcg gtaccccgct tgataactct aacgatgctg
      421 ccgctggtat tagcccgagt gataacagtg gggattatac tggtgcgggt gccgttagtg
      481 cagcgaaagg cgtagctatt cgtttatata accgtgcaga taacactcag gtcaagttat
      541 atgaaaattc tgcatcaact ccgatttcta atggtaatgc atccatgaag ttcatggctc
      601 gttatattgc tacggaaacg actattgacc ctggtacagc taacgccgac tcgcagttta
      661 cagttgagta tataaaataa atcattctta acataatacc ttaaataaaa tggcgctcgc
      721 cattatggtt ctcttttact taaaagaagg gcgtcattat tatattttta tgagatacat
      781 ttatgcctat gtatttactt agattgtttt ttgctgttac tttgattacg cagatgtcga
      841 tggctatggc tggtattgtt gttgggggaa ctcgcgttat tttttatggt gacaaacctg
      901 ataccacaat ctctattttt aataaagaaa cgaaccttcc ttatctattg cagacatggg
      961 ttgatccatt taataaagag gataaaagca aaccgccatt ttctgtgata cctcccgttt
     1021 ctcgcattga accgcagcaa gagaaaatat tacgcatttt aaaaacggct ggcgagttac
     1081 caaaagatcg agagtccgta ttctggttga acgtgaaaaa catccctcct tcaattgata
     1141 aaaaaaatac caatgttctt gagatagcca taaaaacaag aattaagtta ttctggcgac
     1201 cagccgcggt aaagataatc cctgagcagg ctattaaaaa tgttaaatgg tcgcggcagg
     1261 gtaataatct catcgtcgaa aatccaaatc cgatccatat taatgtaatg agtgtgacgg
     1321 tggacgataa agacgtgtcg ctaaatatga tccgcccttt tgaaacattg acattaccaa
     1381 ttccggctgg cgtggcagga aaatcgctcg tttggcgctt tattaatgat tacggtgcaa
     1441 ttagtcaacc actaaagaaa aatctttaag cgccggataa ggatatatcg atggcctctt
     1501 cgcatctttt cattacgctt gcatcgggca tatgtctgct ctgttccata tctgcttttg
     1561 cccgggatag cttgttcaac cccagattac tggaactgga tcatcctgcg gataatattg
     1621 atattcacca gttcaaccgt tcgaatacct tacctgcggg aacatacaaa gttgatgtga
     1681 tgatcaacgg catgctcttc gaacgccagg aagttaaatt tgtccaggat aaccctgatg
     1741 ctgaactcca cccatgctac gtggcgataa aaaacgtact ggcgacctat ggtataaaag
     1801 ttgatgcgat aaaatctctg gcgaatgttg atgacaaaac atgcgtaaat ccagttccgc
     1861 tgatcgacgg ggctacctgg ttactggacg ccagtaaact tgcattgaat attactattc
     1921 cgcaaattta tctcaacaat gcagttaatg gttatatcag cccttcccgt tgggatcagg
     1981 ggatcaatgc catgatgatg aattatgatt tttcggcatc gcataccatc cggtcaaatt
     2041 atgacgacga cgatgacagt tattatctga atttgcgtaa tggtattaat ttaggcgcat
     2101 ggcgttttcg taattacagc accctgaatt cttatgacgg taatgtggac taccattccg
     2161 tcagtaatta cattcagcgc gacattatgg cattacgtag ccagattatg attggcgata
     2221 cctggacggc aagcgatgta tttgatagta cacaggtgcg tggcgtgcgg ctgtataccg
     2281 atgacgatat gttgccctcc agccagaacg gctttgcgcc agtggtacat gggattgcga
     2341 aaactaacgc cacggtgatc atcaaacaaa acggctacgt tatttatcaa tcagccgtac
     2401 cacagggcgc atttgccctc accgacttaa acacgaccag tagcggcggc gatctcgatg
     2461 tcactatcaa agaagaagat ggcagcgagc agcactttat tcagccattt acttcactgg
     2521 ccattctcaa gcgtgaaggt cagaccgatg tagaccttag cattggagaa gtgcgcgacg
     2581 aaagcggctt tacgcctgag gtcttgcagt tacaagcaat gcacggtttc cctttgggaa
     2641 taaccttgta tggcggaaca caactggcaa atgattacgc ttctgccgcg ctgggtattg
     2701 gtaaagatat gggggcgctg ggcgcgattt cttttgacgt gactcatgcc cgctcgcagt
     2761 ttgactacgg cgataatgag agtggtcaat cgtatcgttt tctctattcc aaacgttttg
     2821 aagacaccaa taccaccttt cgtctggtgg gttatcgcta ctctacggag gggttctaca
     2881 ccctcaatga atgggtgtcg cgacaggata acgattctga tttctgggta acgggcaacc
     2941 gtcgcagccg cttcgaaggc acctggacgc aatctttcac gccaggctgg ggcaatattt
     3001 atttaacatt cagtcgacag gaatactggc agaccgatga ggtcgagcgt ttattacagt
     3061 tcggctataa caacaactgg cgaaacatct cctggaacgt ttcctggaac tatacggact
     3121 cgatcaagcg ctcatcgagc aaccatcatg atgataacaa tgataatttc ggcaaagaac
     3181 agattttcat gttctcaatg tcgataccgc tatcgggctg gatggaagac agctacgtca
     3241 actattcgtt aacgcaaaac aaccaccatg aaagcacgat gcaggtcggt ctgaacggaa
     3301 cgatgctgga agggcgtaac ctgtcttata acgtacagga atcgtggatg cactctcctg
     3361 atgactccta cagcggcaat gccggaatga cctatgacgg gacttatggc tcggtcaatg
     3421 gcagctattc ctggagccgt gactcccaac attttgatta tggcgccaga ggcggcgtgc
     3481 tggtgcatag tgacggagtg accttctcgc aggaactggg cgaaacggtg gcattagtca
     3541 aagcgccggg cgcagaaggc ctgtccattg aaaacgccac cgggatttct accgactggc
     3601 gtggttatac cgtaaaaacg cagcttagcc catatgacga aaaccgcgtg gcattgaaca
     3661 gcgactattt ctccaaagcc aatattgaac tggaaaacac cgtcatcaac ctggtaccaa
     3721 cgcgcggtgc ggtggtgaaa gccgaatttg tcacccatgt cggttatcgc gtgctattta
     3781 acgtccgcca ggtcaacggt aaaccaataa tgtttggcgc gatggcaacc gcctctctcg
     3841 aaacgggcac agtcaccggg attgtcggtg ataacggcga actgtatctc tccgggatgc
     3901 ctgaaaaagg cgagttttta ttgagttggg gacaagctgc ggatgaaaaa tgtaaggcgg
     3961 cctatcacat cacccataaa cctgatgata ccagcctggt tcaaatggat gcgatttgtc
     4021 gctaaaggaa gcatgaatga aaaaaatatt atcagggttg attctgctgc tttgctgtcc
     4081 ttatggtttc gccgctaatg gtgatggcgt ttgttttaat agtgatggcg caacgcacat
     4141 gtcaaattta tcatttggtc cgctgacggt ggctgcggcg aataaccact ccggatacaa
     4201 tattttcgag gcactgagca acacgactgg aacatacccg gtgcgctgtc actgtgatga
     4261 cacgcatggc ggaccgggcc aacaaacagc attttttcct atcttctaca cgggggatgc
     4321 cgcaccgggg cttgtgcttg agcgcactct taatgggtta aattactatg ctctgaatga
     4381 ttatttatcg gtcggcgtga cgatttttat tattaataac caatatgcgg ccattccttt
     4441 tgaacactta tccaaccaat ccacctcacc gcaacatacc tgtggagcag gtaataatgg
     4501 gagcactgta aatctggatt cagggcgctc ggcaaaatta tctttctatg ttcggcattc
     4561 tattactggc acggtgacaa tacccacaac ggaagtcgcc tggttgtacg cgggcatgtc
     4621 cgatcatttt cccaaaacga cccccgtttc taaagtgaca attcgcggac aactaacggc
     4681 cccgcagaac tgtgagttaa cgccaaatca gagcatcgat gtcgattttc aaaaaattaa
     4741 tagcgctgag ttctcctcaa cggcgggttc aattattgca gaaagaaaga ttaaaaccga
     4801 agtcacagta tcctgtaccg ggatggaaga cgtaaggtcc acggaggtgg tgagtgcgtc
     4861 gatgattgcg gcaaacagaa gtgccgatgc caccatgatc gtgacgagta atccggatgt
     4921 gggaattaag atttttgata agaacgaccg tccagtgaat gtggatgggg gcaacttacc
     4981 cgctgatatg ggtgctatta gtcgattagg aaaaaccgat ggtagcgtaa cgttttattc
     5041 agcgcccgcc agtctgacgg gcgcaaaacc agcgcctgat aatggattta ccgctacagc
     5101 cacgctggtt attgaattta ctaactaatc ttgtgttagt aaaaggaata atttcatgaa
     5161 aaaatggcat tatatatttt gcataattct ctttcattta gggttgccgt gcgggtatgc
     5221 ggcaaatgat ggcacgtgtg caacaagagg cggcacacat acattaagcc ttaattttcc
     5281 tctgacaacg gtcagtgcag caaacaatgt gcctggaaat acattaatag atattgctaa
     5341 tgcaacatct tctgaaaatt atagcgttct gtgtaactgt gattcaaaac atagcaatgg
     5401 cgcttatcac gaaatatatt ataccgcaga ccctgctccc ggtatggttt atagcaccac
     5461 cgcaagtggt cttgcttttt actatcttaa cgaatatgtc gatgtgggaa caaaaatatc
     5521 tgtgctaaat gcggggtata cggcagttcc ttttgaacat gtttccaacc aggcaactac
     5581 aacagatcac acttgtcagg gaaacaaaac tacagcggtt ggcgtgagcc tgaaaactgg
     5641 agcagatgcg aagatttcat ttcgtattaa acgttcaata aatggaacgg tagtaatacc
     5701 tatcaccgat attgcattgc tgtatgccaa catatccagc accacgaccc gtggtgaggc
     5761 gattgcaaaa gttcgaattt caggcagttt gaccgcacca cagtcttgtc agataaatgc
     5821 aggacaggtg atttattttg attttgatac tattcctgcg tccgaatttt catctaccgc
     5881 cgggcaagcc attacttcac gaaaaatcac taaaacagtg agtattgagt gtacggggat
     5941 gggggatgag cgtacgcaga aagtcgatgc ttcttttacg gggacgaacc gaagcagtga
     6001 cgatacgatg gtggcgacag acaatgctga tgtcgggatc aaaatttaca ataaatcgaa
     6061 tgctgaagtt agcgtcaaca acggcaagtt acccgcagac atgggcaaca cgaccatttt
     6121 tggtcgtaaa aatggttcgg taactttttc ggcagcacct gccagcttta ccggtgcccg
     6181 gcctcagccc ggcgttttta acgctaccgc gaccttaacc attgaatttg taaactaacc
     6241 ttttgcggct gttttatgac agccgttcat atttcctctc ctttcatgaa aaaacgctga
     6301 tatttttacc ctttaatatc aataagtaat aactttcgtt tgggtgtcat caaactgtca
     6361 cattccttac atataactgt cacctgtttg tcctattttg cttctcgtag ccaacaaaca
     6421 atgctttatg aatcctccca ggagacatta tgaaagttat gcgtaccacc gtcgcaactg
     6481 ttgtcgccgc gaccttatcg atgagtgctt tctctgtgtt tgcagaagca agcctgacag
     6541 gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg ggctgacact taccagaaag
     6601 aaaccggtaa taaagttaac taccagggta tcggttcttc cggtggcgta aaacagatta
     6661 tcgctaatac cgttgatttt ggtgcctctg acgcgccgct gtctgatgaa aaattgactc
     6721 aggaaggcct gttccagttc ccgaccgtga ttggcggcgt ggtgctggcg gttaacattc
     6781 cagggttgaa gtctggcgag ctggtgctgg atggtaaaac cctcggcgat atctacctcg
     6841 gcaaaatcaa gaagtgggat gatgaagcca tcgccaaatt gaatccgggt ctgaaactgc
     6901 cttcacagaa cattgctgta gtgcgccgcg cagatggctc cgggacttcc ttcgtcttca
     6961 ccagctacct ggcgaaagtg aatgaagagt ggaaaaacaa cgttggtact ggctctaccg
     7021 taaaatggcc gatcggtctg ggtggtaaag gtaacgacgg tatcgccgcg ttcgttcagc
     7081 gtctgccggg tgcaattggt tacgttgaat atgcttacgc gaagcagaac aacctggcgt
     7141 ataccaaact gatctccgct gatggtaaac cggttagtcc gaccgaagaa aacttcgcta
     7201 atgcagcaaa aggcgcagac tggagcaaaa ccttcgctca ggatctgacc aaccagaaag
     7261 gcgaagatgc gtggcctatt acctctacca cgttcattct gatccacaaa gatcagaaga
     7321 aaccagaaca aggcacagaa gtgctgaaat tcttcgactg ggcgtacaaa accggggcta
     7381 aacaggcgaa cgacctggat tatgccagcc tgccggatag cgtagttgaa caggttcgcg
     7441 cagcgtggaa gaccaatatt aaagacagta gcggtaagcc gctgtactaa taaaactcca
     7501 ggccgggtgc ggtgttttac gccgcatccg gcattacaaa atgactttgt aaacgcgttt
     7561 aactgaagag taacttatgg ctgcaaccaa gcctgctttt aacccaccgg gtaaaaaggg
     7621 cgacataatt ttcagcgtgc tggtaaaact ggcggcgctg attgtgctat tgatgttggg
     7681 tggcattatt gtctctctga tcatctcctc atggccgagc attcagaaat ttggtctggc
     7741 tttcctgtgg accaaagagt gggatgcgcc gaacgatatc tacggggcgc tggtgccgat
     7801 ctacggtacg ttggtgactt cgttaatcgc gctgctgatc gccgtcccgg tgagtttcgg
     7861 tatcgccctg ttcctgactg agcttgcgcc tggctggctg aaacgcccgc tgggtatcgc
     7921 cattgagctg ctggcagcca ttccaagtat cgtttacggc atgtggggcc tgtttatctt
     7981 tgcgccgctg ttcgccgttt acttccagga gccggtcggc aatatcatgt cgaatatccc
     8041 gattgtcggc gcgctgttct ctggcccggc atttggtatc ggtatcctcg cggcaggcgt
     8101 gatcctcgcc atcatgatta ttccgtacat tgcggcggta atgcgcgatg tgttcgaaca
     8161 aaccccggtg atgatgaaag agtcggccta cggtattggc tgcaccacct gggaggttat
     8221 ctggcgtatc gttcttccgt tcaccaaaaa tggtgttatc ggcggcatta tgctggggct
     8281 gggccgcgcg cttggtgaaa ccatggctgt gacctttatc atcggtaaca cctaccagct
     8341 cgacagcgcc tcgctgtaca tgccaggcaa cagtatcacc tctgcactgg cgaacgaatt
     8401 tgcggaagcg gaatccggtc tgcacgttgc cgcactgatg gaactgggcc tgatcctgtt
     8461 tgtgattacc ttcatcgtcc tcgccgcatc gaagtttatg attatgcgcc tggctaagaa
     8521 tgagggggct cgctaatggc tatggttgaa atgcaaacca ctgcggcgct ggcggaatcc
     8581 cgccgcaaaa tgcaggcgcg tcgccgcctc aaaaaccgta ttgcgctgac gctctcgatg
     8641 gcgacgatgg ccttcggtct gttctggctg atctggattt taatgtccac catcacccgc
     8701 ggtatcgacg gtatgtcgct ggcgctgttt actgaaatga cgccgccgcc caatacggaa
     8761 ggtggtggtc tggcgaacgc tctggcgggt agcgggctgt taattttgtg ggccacggta
     8821 ctcggtacgc cgctgggcat tatggcgggg atttatctgg ctgaatatgg tcgtaaatcc
     8881 tggctggcag aagtgattcg cttcattaac gacattctgc tctctgcgcc gtcgattgtg
     8941 gttggtctgt ttgtttacac catcgtggtg gcgcagatgg agcacttctc cggttgggcg
     9001 ggggtgattg cgctggcgtt gttgcaggtg ccgattgtta tccgcaccac cgagaacatg
     9061 ctgaaactgg tgccggacag cctgcgtgaa gcggcttatg cgctgggtac accgaagtgg
     9121 aagatgatct ccgcgattac gctgaaagcg tcggtatccg ggatcatgac cggtatcctg
     9181 ctggcgattg cccgtattgc tggtgaaacc gcgccgctgc tgtttaccgc gctctccaac
     9241 cagttctgga gcacggacat gatgcagccg atcgccaacc tgccggtcac catctttaaa
     9301 tttgcgatga gcccgtttgc tgaatggcag caactggcct gggccggggt gttgattatt
     9361 accctgtgcg tactgctgct gaacattctg gcgcgcgttg tttttgcgaa gaataaacac
     9421 ggttaacact ttgccggatg cggcgtgaac gcctgatccg gcctacggta agcctgatta
     9481 gcgaagtgca tcaggcacga tgaggaaaag attgcaatga gtatggttga aactgccccg
     9541 agtaaaattc aggttcgtaa tttgaacttc tactacggca aattccatgc cctgaaaaac
     9601 atcaacctgg atatcgctaa aaaccaggtt acggcgttta tcgggccgtc cggctgcggt
     9661 aaatcgacgc tgctgcgtac cttcaacaaa atgtttgaac tgtacccgga gcagcgcgca
     9721 gaaggtgaaa ttctgcttga tggcgacaac atcctgacca actctcagga tatcgccctg
     9781 ctgcgtgcga aagtgggcat ggtgttccag aaaccgacgc cgttcccgat gtccatctac
     9841 gacaacatcg cctttggcgt tcgtctgttt gagaagctct cccgtgccga catggacgag
     9901 cgcgtgcagt gggcattgac caaagccgca ttgtggaacg aaaccaaaga taaattgcat
     9961 cagagcggtt actctctctc tggcggtcag caacagcgtc tgtgtattgc gcgtggtatc
    10021 gccattcgcc cggaagtgct gctgctcgac gaaccgtgtt cggcgctcga ccctatctct
    10081 accgggcgta ttgaagagct gatcaccgaa ctgaagcagg attacaccgt ggtgatcgtc
    10141 acccacaaca tgcagcaggc tgcgcgttgt tccgaccaca cggcgtttat gtacctgggc
    10201 gaattgattg agttcagcaa cacggacgat ctgttcacca agccagcgaa gaaacaaaca
    10261 gaagactaca tcaccggtcg ttacggttga ttcaggagtg cgttatggac agtctcaatc
    10321 ttaataaaca tatttccggc cagttcaacg ccgaactgga aagtatccgc acgcaggtga
    10381 tgaccatggg cggcatggtg gagcagcagc tttctgatgc aatcaccgcg atgcacaacc
    10441 aggacagcga tctggcgaag cgcgtcatcg aaggcgacaa gaacgtcaac atgatggaag
    10501 tggcgatcga tgaagcctgc gtgcgcatta ttgccaaacg tcagccgaca gcgagcgacc
    10561 tgcgtctggt tatggtgatc agtaaaacca ttgctgaact ggagcgtatt ggcgacgtgg
    10621 cggacaaaat ctgccgtact gcgctggaga aattctccca gcagcatcag ccgttgctgg
    10681 taagtctgga gtcgctgggc cgtcatacca tccagatgct gcacgacgtg ctggacgcgt
    10741 tcgcgcggat ggacattgac gaagcggtac gtatttatcg tgaagataaa aaagtcgatc
    10801 aggagtatga aggtattgtt cgtcaactga tgacctacat gatggaagat tcgcgtacca
    10861 ttccgagcgt acttaccgcg ctattctgtg cgcgttctat cgaacgtatt ggcgaccgct
    10921 gccagaatat ttgtgagttt atcttctact acgtgaaggg gcaggatttc cgtcatgtag
    10981 gcggtgatga gctggataaa ctgctggcgg gaaaagaccc gaaagagtag tgctaactgt
    11041 aggccagata aagctttttg ccgcatctgg caatcagccc ggtcatacat agttaccggg
    11101 ctgataaact ttctcactga aatattgcca cacaaactca cctcgaaact ctccccagta
    11161 cattgcatat acattattac atttgaattt ttttactttt aatgtagtgc cccgctataa
    11221 aaacattttt gttattcttt tcatttatat cgctacctgc aaagaaaatg gaaaagaaaa
    11281 caaaaaccag ggtaaaagcc ctatatcgaa acataaaaaa tgagcgattt tttacagtat
    11341 tcataggtgg tttagtcata atcgtagcaa accaacaccc aggtatgctt tttaagacaa
    11401 atcggcaatg tattgtgctc gggcgaataa atattgtgtt caggagaaat gattatggct
    11461 atatcatcat atagtacagt tacagataaa tttgctaatc tgactaaaat gccagatgaa
    11521 aataatggat tgcaaaaaaa atcagaaaaa ttttgtttgt ctgttggtaa taaggagaag
    11581 tattcaataa cccataaaga tataacaaac cttatagctg gtgatagaca atcttttctt
    11641 aaactaactc tttgggaatt tatactcgat ctctttccca tgtatcatat taaggaggct
    11701 aaagaaacaa ttgtagaatt tgtaacgaaa actgaggaaa aagttaagag atttgagaga
    11761 attaaatctc tcgctattcc agaagaaaaa tggcgcttta gtacgacgac agaatttaca
    11821 gtagatgaga ataaagacat tattgttagt agagtattta aaa
//
LOCUS       DAZFFG010000130        11807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFFG010000130 DAZFFG010000000
VERSION     DAZFFG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.130"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            77..1132
                     /gene="phoE"
                     /locus_tag="ACHJ7J_004132"
     CDS             77..1132
                     /gene="phoE"
                     /locus_tag="ACHJ7J_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5002300.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1171..1572)
                     /gene="crl"
                     /locus_tag="ACHJ7J_004133"
     CDS             complement(1171..1572)
                     /gene="crl"
                     /locus_tag="ACHJ7J_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5002301.1"
                     /translation="MTLPSGQPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="ACHJ7J_004134"
     CDS             complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="ACHJ7J_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5002302.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITEWIEKRLC"
     gene            complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="ACHJ7J_004135"
     CDS             complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="ACHJ7J_004135"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5002303.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3685..5142
                     /gene="pepD"
                     /locus_tag="ACHJ7J_004136"
     CDS             3685..5142
                     /gene="pepD"
                     /locus_tag="ACHJ7J_004136"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5002304.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5199..5480)
                     /locus_tag="ACHJ7J_004137"
                     /pseudo
     CDS             complement(5199..5480)
                     /locus_tag="ACHJ7J_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(5527..5733)
                     /locus_tag="ACHJ7J_004138"
                     /pseudo
     CDS             complement(5527..5733)
                     /locus_tag="ACHJ7J_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(6052..6504)
                     /gene="yafP"
                     /locus_tag="ACHJ7J_004139"
     CDS             complement(6052..6504)
                     /gene="yafP"
                     /locus_tag="ACHJ7J_004139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5002305.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEYYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(6501..7556)
                     /gene="dinB"
                     /locus_tag="ACHJ7J_004140"
     CDS             complement(6501..7556)
                     /gene="dinB"
                     /locus_tag="ACHJ7J_004140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5002306.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7627..8412)
                     /gene="lafU"
                     /locus_tag="ACHJ7J_004141"
     CDS             complement(7627..8412)
                     /gene="lafU"
                     /locus_tag="ACHJ7J_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5002307.1"
                     /translation="MTTIKLIVNSVSKFERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            8384..10096
                     /locus_tag="ACHJ7J_004142"
                     /pseudo
     CDS             8384..10096
                     /locus_tag="ACHJ7J_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(10289..10786)
                     /gene="rayT"
                     /locus_tag="ACHJ7J_004143"
     CDS             complement(10289..10786)
                     /gene="rayT"
                     /locus_tag="ACHJ7J_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO5002308.1"
                     /translation="MSEYRRYYIKGGSWFFTVNLRNRRSHLLTAQFQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGNITDFSAG
                     ERIIS"
     gene            complement(10962..11711)
                     /gene="yafL"
                     /locus_tag="ACHJ7J_004144"
     CDS             complement(10962..11711)
                     /gene="yafL"
                     /locus_tag="ACHJ7J_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5002309.1"
                     /translation="MSLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         2906 a         3060 c         3000 g         2841 t
ORIGIN      
        1 caggttaaaa actttcctgt tttcaacggg gctctcccgc tgaatattcg cgcgttaatt
       61 aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc
      121 atctgcatcc gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta
      181 tggcaaagtt aaagccatgc attatatgag tgataacgac agtaaagatg gcgaccagag
      241 ttatatccgt tttggtttta aaggcgaaac acaaattaac gatcaactga ctggttatgg
      301 tcgttgggaa gcagagtttg ccggtaataa agcagagagt gatactgcac agcaaaaaac
      361 gcgtctcgct tttgccgggt tgaaatataa agatttgggt tctttcgatt atggtcgtaa
      421 cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga
      481 ctcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg
      541 gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa
      601 aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata
      661 tgactttggc ggcagcgatt tcgccattag tggggcctat accaactcag atcgcaccaa
      721 cgagcagaac ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg ctacaggtct
      781 gaaatacgat gccaataata tttatctggc aactttttat tctgaaacac gcaaaatgac
      841 gccaataact ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca
      901 gtttgacttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga
      961 aggtatcggt gatgaagatc tggtcaatta tatcgatgtc ggtgctacat attatttcaa
     1021 caaaaatatg tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt
     1081 gaatattaat aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatgattatt
     1141 gccggatgtg atgcatccgg cacatttcac tcacgccgtt aacttcaccg gctcgtcacg
     1201 aaaatcatcc gccggttcca gcttcagatt cagcgtcgtc agcagttcac gcagcttctc
     1261 gtgaaactca cgcagggtgt gctccagtcg ttcaaccact tcagtgtctt ttaccggaac
     1321 actcttccag tcgcctgctt tatcgaacag accaaactgg taactgtagg taaaacggga
     1381 ttcctgcgct tcaagctcca tccaccagcc ccagaactca cgcacttccg gtgccggttt
     1441 cacgttgacg catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc
     1501 acgaatatac gggcctagtg cggtaaattt tttgatcaat ctgctcttcg gttgtccact
     1561 cggtaacgtc attgctatct cctgttgtga tgcaactgtt ttaccaaatt ggcaaaattt
     1621 agcaaatttt taacacaaac gtttttcgat ccattctgtg atttcctgaa gacctttgtc
     1681 aaaattccga tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgatga
     1741 ggtgattaag cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa
     1801 cattggcgtt gggcagcgac gtcccagcaa tccttgcact tttaatgaat agcgattcag
     1861 ctccacgcgc aacgcttcgt cggaagcatc atgcatcccc aaacgactgg ccagaacgtc
     1921 aagatacatt tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac
     1981 cggaccaaga caggcaaccg ctttcagacg cggtgattca aggtatgcca gacgcacggc
     2041 aacgttagcg ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac
     2101 gttaggcagt gcctttaaga cgtgctgatg caacaggctg gagtcctggg tgagcttcca
     2161 ttttgaagaa aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcggcgc
     2221 aaaataacgt tcatacaggc tgtaatagtc cgtctgcatc gcatccagac caccacacat
     2281 taataccgtc gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc
     2341 accgccaggt acggtaaact ccatctgccg catcgtgccc ggcagacgct gagcggcctc
     2401 ttcataggcg cggtttgaca aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg
     2461 aggataggcg gcaatgttgt acaacgtagc cgcatgcagc cagtgacgac cactgagttg
     2521 cggatcttct tccgcacagg ctttttgttg ccacaccatc gcctgggtgg cccactcata
     2581 aatccagttg ccgccacggt agccaatcac cgtatcgtat aaatcatcgt cggtacgttc
     2641 ggcatcgctc atcacaatac gtgcctggac gtcgaggatt tcgcgtgggt caatgccgcg
     2701 ccagatccac atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag
     2761 ggccgactgc acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc
     2821 aggatgttta aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc
     2881 agcctccaga atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc
     2941 ccggcagtgc caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc
     3001 ccatatccca cggctgttcg atccaggtat cttgcgggat atcaacaaca taatcatcaa
     3061 ccagcggacg accagccggt tttgcaaaga tggtgacaaa gtgcgctttt ggatacattt
     3121 cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt
     3181 cgccatcgcc ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc
     3241 tggaaataca aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg
     3301 gtaccagacc gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc
     3361 ggcttgcgag tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc
     3421 tcatgtgaag tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag
     3481 gttgcgcgag attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt
     3541 ctttttacgc actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc
     3601 aagcctgtct tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc
     3661 gggtgatcga caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg
     3721 tgggatattt ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc
     3781 gctgaataca ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta
     3841 ggtaatatcc tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa accggtcgtc
     3901 ttgcaggccc acctcgatat ggtgccgcag aaaaataacg acaccgtgca tgacttcacg
     3961 aaagatccta tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg
     4021 ggtgcggata acggcattgg tatggcctct gcactggcgg ttctggctga cgaaaacgtg
     4081 gttcacggtc cgctggaagt gctgctgacc atgaccgaag aagccggtat ggacggtgct
     4141 tttggcttac agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa
     4201 gaaggtgaaa tctacatggg ttgcgcgggg ggtatcgact tcacctccaa cctgcattta
     4261 gatcgtgaag cggttccagc tggttttgaa accttcaagt taaccttaaa aggtctgaaa
     4321 ggcggtcact ccggcgggga aatccacgtt ggcctgggta atgccaacaa actgctggtg
     4381 cgcttcctgg cgggtcatgc agaagaactg gacctgcgtc ttattgattt caacggcggc
     4441 acactgcgta acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa
     4501 gtcgacgccc tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa cgagctggca
     4561 gagaaagaga aaaatctggc cttgttgctg gactctgtag cgaacgataa agctgccctg
     4621 attgcgaaat ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc
     4681 cgcaactccg acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc
     4741 atgactgaca ataacgtaga aattcactgt ctgatccgtt ctctgatcga cagcggtaaa
     4801 gactacgtgg tgagcatgct ggattcgctg ggtaaactgg ctggcgcgaa aaccgaagcg
     4861 aaaggcgcat atcctggctg gcagccggac gctaattctc cggtgatgca tctggtacgt
     4921 gaaacctatc agcgcctgtt caacaagacg ccgaacatcc agattatcca cgcgggcctg
     4981 gaatgtggtc tgttcaaaaa accgtatccg gaaatggaca tggtttctat cgggccaact
     5041 atcaccggtc cacactctcc ggatgagcaa gttcacatcg aaagcgtagg tcattactgg
     5101 acactgctga ctgaactgct gaaagaaatt ccggcgaagt aattatttga ttttctgccg
     5161 gatggcgttt aatcgccttc cggcagtttt atccttcatt atccttcgat aaaagccatc
     5221 cctgtaaacg ttcgtcgcgg gttgccacgt tcaatctggt gatggaacat tcgccgctgt
     5281 gatttcagcg ccgcgctatt ttcctgttgc tgctgctcca gcttccaggc aatcagcaat
     5341 cgcgccagcc gtttgttagc atgctggctg cgttctgact gaaccttcac gctaataccg
     5401 gatgccagat gcgtagcgcg taccgccgag tcggttttat tgacatgttg accgcccggc
     5461 cccgacgaac gcagcgtgtc aggataacac ccggcttcgg tttccagcac cgttaccgcg
     5521 acgtcttgcc gggtaaaaac gaccaacaga gaagaacgct gcgccaatcg ggtagccgcg
     5581 tccggggtgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
     5641 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
     5701 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccatttata ctcgtcatac
     5761 ttcaggttgc atgtactgcg actgcgttcg ttcaccccag tcacttactt tagtaagctc
     5821 ctggcgattc actcgcttgt caccttcctg caactcgaat tatttagagt atataaatat
     5881 taaaaatcag gaaatggcag acgaatccag ggcaagaaaa aaagaattaa tcctggaaag
     5941 gtctgcaaag agtcattgat aaaggatttg tcatgattta cctccttgca gtttgtggaa
     6001 tcagaaaacg cgcacatacc aggcgggcgt tattttcatt gcaagctgga tttaatgttg
     6061 cggtttatat cgcatataaa aattagtaaa ccacgctccc cggcattcaa cgcgctgctg
     6121 cttaactgtc tgaaaaccat aacgttcaaa aaagggtttt gcggttatgc ttgcgtccac
     6181 cgtaagttcg gattcagact taatcaaagg ttttaacaaa gcgctggcaa ccccacggcg
     6241 ggtgtattca gggtcaacaa ataacatatc gatatagtat tcaacgcagg taataaagcc
     6301 gaccagtttt gcattaatga ctgcaacccg cacttgtgat ttcgcgagtt tctccttcca
     6361 gcgagattcg tcaatctgcg cccaggcggc aatttgttgt ggtgaataat gctgactggc
     6421 ggtcatcgta accgctctaa ggaaaatagc gcatagttgc tgaaaatcgc caggctgata
     6481 gtttcttatt tgtatgttat tcataatccc agcaccagtt gtctttccat ttgcggatcc
     6541 agcaacgtca cgtgcagccc caccagacgc acacctcgcc cgccgcggcg ttcatcccag
     6601 gttttacgcg cggtggcgat taagtcagct ttattcagcc gcggccagac gtgctcctga
     6661 gtggtttgct gaaaatcgtc gaactttaat ttcactccct ggcgagcaat cagtaaatca
     6721 ggttttacct ttgccagacg gcgttcaagt tccggataca gccgctcgat aatcgcttca
     6781 cattcagacc agtgatgaat atcttccgcc atcgtgcgtt ccacgccgac ggattttcgt
     6841 aaccgttcgc tgttaacgtc gcgctcgtca atcccctgac tacgctccca caaaatgcgg
     6901 ccaaatttgc caaagcgttt aagcagaatc accagatcac acttttgtac atcaccgcag
     6961 gtccgcagcc ccatcgcttc cagttttgcc gccgagactt tgccgacgcc ggggattttt
     7021 gccagtggta aggtttgtaa aaatgtcgga acttctgccg gcgtaatcac aaactggccg
     7081 ttgggtttat tcatgtctga ggcgattttg gcgagaaact ttactggtgc cacgcccgca
     7141 gatgccgtca gttgcagctc gttgaagatc gtctggcgga tttcctgggc gatgagggtc
     7201 gcagaaccgt ggcaatggac gctatcggtg acatcgagat aagcctcatc cagtgacaac
     7261 ggttcaatac gcgaggtgta gcgcgagaag atttcacgga tatgatttga ggcttctttg
     7321 taggcgtcaa agcgccccgg aagcaaggtg agatgtgggc ataatttgag cgccatccct
     7381 gtcggcatag cgctgcgtac gccaaattta cgcgcgggat aattggcggt gctgatcacc
     7441 ccccgacgtt cgcggctgcc gccaatagca atagggatat cgcgcagggc gggattgtcg
     7501 cgcatctcca ctgcggcgaa aaagcagtcc atatccacat gaatgatttt acgcattact
     7561 cacctctcaa cactggtaaa gtatacagtg atttcagggt ttgagaaatg cgtaaagatt
     7621 cagcatttag cgcgttcgct gcgaaagcac ctgctgctta tccagctttt gcaccagcgg
     7681 ctgcaccact ttatccccat gctgaccaaa gtattgatac agcgtatcgg acgcactttt
     7741 ggtcagcacc ataatctcaa tgcgtcggtt gcccgcgctt tgcggatttt tcgcgtccag
     7801 cagcatctgg tccgccatcg cgctcacctg catcacttta tcttccggca ttccggcctc
     7861 ttccagcacc cgacgagccg aaagcgcgcg atcgcccgaa aggttccagt tgttgtagat
     7921 attgtttttg taggccatcg catcggtatg cccggtaata ataattttgt tatcgagcga
     7981 gtcgaacact ggcgcgagct ccaccagcag cgttttaaag aacggcataa tctgcgcgct
     8041 gccgcgttca aacatattgc ggttctggtc gtctttaatc agcacgcgta atccctgcgg
     8101 gacaatctcc atctccagat tcgcttccat atgcgcatca cgggcaatgg tgttgatact
     8161 ggttgccaac tcgccaagct cggtggcgga tttcttcttc agcagcgccg ttttctcatt
     8221 cacatcacgc gcttttttct ccgtctcttc cggcaccgcc acggtggcag gttttggcgg
     8281 atgagacggg ctgattttat ttaacggcga cagcccacca ccgttaaaaa tcgactgccc
     8341 gtgcagcgcg gcgataatgc tttcacgctc gaatttactg acgctgttga caatcaactt
     8401 tatcgtcgtc accaaagggg ccgagcgtat ttctgaggtt tctgcccgct ttaccctgga
     8461 cgcgatgccc ggcaagcaga tggcgattga cgccgatctt aacgccgggt tgatcaatca
     8521 ggcgcaggcg caaacccggc gtaaagatgt cgccagcgag gccgatttct acggcgcgat
     8581 ggacggggca tcgaagtttg tgcgcgggga cgccatcgcc gggatgatga ttctggcgat
     8641 caacctgatc ggcggcgtct gtatcgggat atttaaatac aacctgagcg ccgacgccgc
     8701 cttccagcaa tatgtgctga tgaccatcgg cgatgggctg gtggcgcaga tcccttccct
     8761 cctgctctcc accgcagcgg cgattatcgt cacccgcgtc agcgacaacg gcgatatcgc
     8821 ccatgacgtg cgccaccagc tgctggcaag cccgtcggtg ctctacaccg ccaccgggat
     8881 tatgtttgtg ctggcggtgg tgccgggaat gccgcacctg ccgtttttgc tgttcagcgc
     8941 cctgcttggc tttaccggct ggcggatgag caagcgcccg caggcggcgg aggcggaaga
     9001 aaaaagcctc gaaacgctga cccgcaccat caccgaaacc agcgagcaac aggtcagttg
     9061 ggaaaccatt ccgctgatcg agcccatcag cttaagcctc ggctacaaac tggtggcgct
     9121 ggtggacaaa gcgcagggca acccgctcac ccagcgtatt cgcggcgtac ggcaggtgat
     9181 atccgacggt aacggcgtac tgctgccgga gatccgcatt cgggaaaact tccgccttaa
     9241 gccgagccag tacgccattt tcatcaacgg tattaaggcc gatgaagcgg atattccggc
     9301 ggataaactg atggccctgc cctccagcga aacctacggc gagattgacg gcgtgctggg
     9361 gaacgacccg gcgtacggga tgccagtgac ctggatccag gcggcgcaga aagcgaaggc
     9421 gctgaatatg gggtatcagg tgatcgacag cgccagcgtg attgccaccc atgtgaacaa
     9481 gattgtccgc agctatattc ctgatttgtt taactatgac gacatcacac agttgcataa
     9541 ccgtttgtcg tcgatggcac cgcgcctggc agaagactta agcgcggcgc tgaattacag
     9601 ccagttgctg aaagtgtacc gcacactact gaccgaaggc gtttccctgc gcgatatcgt
     9661 caccatcgcc accgtgctgg tcgccagtag cgcggtgact aaagatcata ttctgctggc
     9721 ggccgatgta cgcctggcgc tgcggcgcag cattacccat ccgttcgttc gcaagcagga
     9781 gctgacggtg tatacgctga ataatgagct ggaaaatctg ctcaccaatg tggtgaatca
     9841 ggcgcaacag ggcggcaaag tgatgctcga cagcgtgccg gttgacccga atatgctcaa
     9901 ccagttccag agtacgatgc cgcaggtaaa agagcagatg aaagcggcgg ggaaagaccc
     9961 ggtgctgctg gtgccgccgc agcttcgccc tttgctggcg cgctatgcga gattgtttgc
    10021 gccggggctg catgtgctgt cgtataacga agtgccggat gagctggagt tgaagattat
    10081 gggggcgctg atgtaggtat ccggcatcgg tgcacaaccg ccggatgcgg cgtgaacgcc
    10141 ttatccggcc taccgatccg gcacaggttt gtaggcatga taagacgcgc cagcgtcgca
    10201 tcaggcatcc gcgcacaatt gccggatgcg gcacaagttt gtaggcatga taagacgcgc
    10261 tagcgtcgca tcaggcatcc gcgcacaatt atgaaatgat acgctccccg gcactaaaat
    10321 ccgttatgtt acccgcccaa tcaatgggat ataaccctcg cgccacatcg cgatggaacg
    10381 ttgagaatgg ccaatcactc acttgcttta cccagccatg tttcactgga tttatataaa
    10441 tataatcaac atgatgccga taatcttttg tattacggat ggcatgctcc caaaaacgcg
    10501 gttgccagat atttttcaat ccacaagcat gggtgaattg ctttttaatc tcccgccagc
    10561 gagaggaaaa atcatcatcg ctttcaggta atgtccagat acagtgcata tgctccggca
    10621 aaactaccca ggcattaatt tcaaaaggtc tgtctcgctt aactttaata atagcgttac
    10681 ggagcatctg gaactgagcg gtcagaaggt ggcttcgacg atttcgtagg ttaaccgtga
    10741 aaaaccagct tccccctttg atgtaataac gacgatattc agacatggcg actccttttt
    10801 ctcaacggta agaagaaaag gaaaggtcgg caatgaggtg atggaataac aggaaagcgg
    10861 ctcgcaaaaa cgcacagatt tgtaggcatg ataagacgcg ccagcgtcgc atcaggcatc
    10921 tgcgtacaac tgccggatgc ggcgtgaatg ccttatccgt cctacaaaat cgtctcctcc
    10981 gtcaaaatcc ggcgcgcgcc taaaaaatga tcctgccaga aaggttcagc taaccgactt
    11041 acccgaatgg tttccccggt gcgcggtgac tcaataaact gaccgtcgcc cagatacacc
    11101 cccatatggt cggcaatctc gcggctgtgg atatggaaaa acagcaaatc tccccggcgc
    11161 aggtcgttat tcgccacaat cgttgcccga tggtagtgat acatctcatt agccgtacgc
    11221 gggagcttcg cctcgaggat cttgttgtag gcataaaaaa ccaacccgct acagtcgaag
    11281 cctttatcgg gccgcgtgcc gccccagacg taaggcttgc caagctgctg ctccaggcgg
    11341 tgaatcgcca cttcggtgat gttatgcagg tgatcgctgc ttaaaaagtg gttgttttcc
    11401 gccagcgcct gccagcgtct gtcactcgcc ttgagcggcg caggaaacca ttctggatgc
    11461 tgttttatct gctcccggtt gtgctggcgt agcgcccttc tgctttcggc attgccttcc
    11521 acaatatagc tggcctgttt tttcagatgg gtttggtact gttttaataa acgcgcacgg
    11581 ttttgcatcc gctggcgggc ggcgtagctg aaagagatat gtgatgtggt ggcgctggca
    11641 aatgaagaaa acggcaagca aagcaacagt aatcccgaca acacaaaaga gggaatcgac
    11701 ggcaaggaca tactcaataa tggctggtca tgcattgatg ttacagtgta tcccggcccg
    11761 tacctgaaca aacttaataa atcattatta taaaattaat aaatata
//
LOCUS       DAZFFG010000131        11382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFFG010000131 DAZFFG010000000
VERSION     DAZFFG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.131"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            185..1573
                     /gene="narU"
                     /locus_tag="ACHJ7J_004145"
     CDS             185..1573
                     /gene="narU"
                     /locus_tag="ACHJ7J_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO5002310.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1668..5408
                     /gene="narZ"
                     /locus_tag="ACHJ7J_004146"
     CDS             1668..5408
                     /gene="narZ"
                     /locus_tag="ACHJ7J_004146"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO5002311.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDHWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLCESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGELRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5405..6949
                     /gene="narH"
                     /locus_tag="ACHJ7J_004147"
     CDS             5405..6949
                     /gene="narH"
                     /locus_tag="ACHJ7J_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5002312.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6949..7644
                     /gene="narW"
                     /locus_tag="ACHJ7J_004148"
     CDS             6949..7644
                     /gene="narW"
                     /locus_tag="ACHJ7J_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5002313.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWSSLFDALLQLAGS
                     SLSSDSVTKQIRSEERDDTPQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7641..8321
                     /gene="narI"
                     /locus_tag="ACHJ7J_004149"
     CDS             7641..8321
                     /gene="narI"
                     /locus_tag="ACHJ7J_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5002314.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8401..9294
                     /gene="yddE"
                     /locus_tag="ACHJ7J_004150"
     CDS             8401..9294
                     /gene="yddE"
                     /locus_tag="ACHJ7J_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO5002315.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKALGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGKTRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9390..10235)
                     /gene="nhoA"
                     /locus_tag="ACHJ7J_004151"
     CDS             complement(9390..10235)
                     /gene="nhoA"
                     /locus_tag="ACHJ7J_004151"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO5002316.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVTARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVPDLVQITPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENEHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10408..10977
                     /gene="yddH"
                     /locus_tag="ACHJ7J_004152"
     CDS             10408..10977
                     /gene="yddH"
                     /locus_tag="ACHJ7J_004152"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO5002317.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEQYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10981..11208)
                     /gene="pptA"
                     /locus_tag="ACHJ7J_004153"
     CDS             complement(10981..11208)
                     /gene="pptA"
                     /locus_tag="ACHJ7J_004153"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO5002318.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11307..>11382)
                     /locus_tag="ACHJ7J_004154"
     CDS             complement(11307..>11382)
                     /locus_tag="ACHJ7J_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002319.1"
                     /translation="RRKMRKAAMDRNYLASVLAGRRLS"
BASE COUNT         2668 a         2975 c         2992 g         2747 t
ORIGIN      
        1 cagttacatt tagtaacaca tatctggcta cctaatcccc attctgcccc cgctgttacg
       61 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
      121 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
      181 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
      241 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
      301 agtcagttgt ctacttcttg ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa
      361 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ctgcattacc
      421 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtaccta tattcggtgg
      481 acgccgatgg acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
      541 tgccgtgcag aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
      601 ttttgcaggt gcgaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
      661 caggcaaggt agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtcat
      721 gcagctggtt gcaccgctgg tcatttttgt acccgtattt gcctttctcg gcgtcaatgg
      781 agtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
      841 gctactagcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
      901 cgcgtcaatt gccgaccagc tccctgtgtt acaacgcctg catctctggc tgttgagcct
      961 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggtttcg ccatgctggc
     1021 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg
     1081 tgccatcgcg cggtcggtgg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     1141 gttgatcaac tttattttta tggcgatttt cagcgccctg ctgttcctta ccttaccggg
     1201 cacaggctcc ggtaatttca tcgcatttta cgccgtattt atggggctgt ttctgaccgc
     1261 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     1321 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ctatcaccga
     1381 aacggcggcg gctctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     1441 gcaggcattt ggcatgtcgc tcaatatgac cggctctcca gtgggcgcga tgaaagtgtt
     1501 tttaatcttc tacatcgttt gtgtgctact gacctggctg gtttatggtc ggcggaaatt
     1561 cagccaaaaa taagagatcg atgcctgaca cgtcttatca ggctacaacc gtgtcgcatc
     1621 cgacaaggca tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt
     1681 tggatcgctt tcgctacttc aaacaaaagg gcgatacctt tgccgatggt cacggacagg
     1741 tgatgcatag caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa
     1801 tcgtgcgttc cacccacggt gttaactgta caggctcctg tagctggaaa atctacgtta
     1861 aaaatggtct ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc
     1921 tgcccaatca tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctttaca
     1981 gcgctaaccg cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgcgaag
     2041 ccctcaaaca acacagcgat ccggtacttg cgtgggcatc gattatgaac gatccgcaaa
     2101 agtgcctgag ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg
     2161 aactaaacca gctgattgcc gccgctaacg tctggaccat caaaacctac ggcccggatc
     2221 gcgttgccgg tttctcgccg atcccggcga tgtcgatggt ttcttacgcc gccggaacac
     2281 gttatctgtc actattaggt ggcacctgtt taagcttcta cgactggtat tgcgacctgc
     2341 cgcccgcctc gccgatgacc tggggcgagc aaaccgacgt gccggaatct gccgactggt
     2401 ataactccag ttacatcatc gcctgggggt ctaacgtacc gcagacacgt acgccggacg
     2461 cccatttctt taccgaagta cgctacaaag gcactaaaac catcgccatt acccctgact
     2521 actctgaagt ggccaaattg tgcgaccatt ggctggcacc aaaacaaggc actgatagcg
     2581 ccctggcgat ggcaatgggc catgtgattt taaaagagtt tcatctcgat aatcccagcg
     2641 actactttat caactactgc cgccgctata gcgacatgcc gatgctggta atgctggaac
     2701 ctcgcgacga cggtagctac gtacccgggc gcatgatccg cgcgtctgac ctggtagatg
     2761 gactatgcga aagcaataat ccgcagtgga aaaccgtggc ggttaatacc gcaggtgaat
     2821 tggtagtgcc gaatggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg
     2881 aatccattgc cgccggtaag gaaaccgaat tgtcgttaac cctgctcggt caacatgacg
     2941 ccgttgcagg cgtggctttc ccctactttg gcggcatcga aaatccgcat tttcgcagcg
     3001 taaaacacaa tcctgtcctg gtgcgtcaat tacccgttaa aaacctgaca ttagccgatg
     3061 gcagcacctg tccagtggtc agcgtttatg atttggtact ggcgaattac ggcctcgatc
     3121 gcgggctgga agatgaaaac agcgcgaaag attacgctga aatcaaaccg tacacccctg
     3181 cctggggcga gcaaattacc ggcgttccgc gccagtacat tgaaaccatc gcccgcgaat
     3241 ttgccgatac cgctcataaa acgcatgggc gctcgatgat tatcctcggt gctggtgtca
     3301 accactggta tcacatggac atgaactacc gcgggatgat caacatgctg atcttctgcg
     3361 gttgtgtcgg acaaagcggt ggcggctggg cacactatgt cggtcaggaa aaactgcgcc
     3421 cgcaaaccgg ctggttgccg ctggcctttg cgctcgactg gaaccgacca ccacgccaga
     3481 tgaacagcac ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg
     3541 cgcaggagtt actttcaccg cttgccgatg ccagtaagta cagcggtcat ctgattgatt
     3601 tcaacgttcg cgccgaacgc atgggctggc taccttctgc gccgcagctg gggcgtaacc
     3661 cgctcgggat taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc
     3721 aggcgctgaa atcgggcgag ttgcgtatgg cctgcgaaca accagatagc ggcagcaatc
     3781 atccgcgtaa tttgtttgtc tggcgttcta acctgcttgg ttcctccggc aaaggccacg
     3841 agtatatgca gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg
     3901 ccagcgacgg gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc
     3961 tcgacctgct ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg
     4021 ttctgcccac cgccacctgg tatgaaaaag acgatatgaa cacctcggat atgcatccgt
     4081 ttattcatcc gctatctgcg gcggtcgatc cggcctggga atcacgcagc gactgggaaa
     4141 tctacaaagg tattgccaaa gcattttcgc aagtgtgtgt gggtcatctt ggcaaagaaa
     4201 ccgacgtggt attgcaacca ctgctgcacg actctccggc agagctctca cagccgtgtg
     4261 aagtcctcga ctggcgcaaa ggcgaatgcg atctgattcc aggtaaaacc gcacccaata
     4321 ttgtggcggt ggagcgcgac taccctgcta cctatgaacg ctttacctcg ctcgggccat
     4381 tgatggacaa acttggcaac ggcggtaaag ggatttcgtg gaatacgcag gatgaaatcg
     4441 atttcctcgg taaactcaat tacaccaagc gtgatggccc agcgcagggg cgtccgctga
     4501 ttgacaccgc cattgacgct tcagaagtga ttctggcact ggcaccagaa accaacggtc
     4561 atgttgcagt taaagcgtgg caggcgctgg gcgagatcac cggacgcgaa catacccatc
     4621 tggcgctgca caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta
     4681 aaattatctc cagccccaca tggtctggtc tggaaagcga tcacgtctcc tataatgcgg
     4741 gatacaccaa cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcaactct
     4801 atcaggatca tccgtggatg cgagcttttg gtgaaagcct ggtggcttat cgcccgccta
     4861 ttgacacccg tagcgtcagt gaaatgcgcc aaatcccgcc aaacggcttc ccggaaaaag
     4921 cacttaactt cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc
     4981 tgctaatgct gacgctttct cgcggtggac cgattgtctg gatcagcgaa accgatgccc
     5041 gagaattgac cattgtcgat aacgactggg tggaagtgtt taacgccaat ggcgcgctga
     5101 cggcccgcgc ggtggtcagc caacgtgtac cgccgggtat gaccatgatg tatcacgccc
     5161 aggaacgcat tatgaatatc cctggttcgg aagtaactgg catgcgcggc ggcattcata
     5221 actcggttac ccgcgtttgc ccgaaaccaa cgcatatgat tggcggttac gcgcagctgg
     5281 cctggggctt taactactac ggcaccgtcg gctcaaaccg tgatgaattc atcatgatcc
     5341 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     5401 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     5461 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggta tggagtacgc
     5521 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     5581 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatacgcc cgcgtctggg
     5641 cagcaagatg ggtgtaataa ccaaaatctt cgccaacccg gtggtgccgc agattgatga
     5701 ttactacgaa cctttcacct tcgactacga acatttgcat agcgcaccgg aaggcaaaca
     5761 tatccctact gctcgcccgc gttcgctgat tgacggtaag cggatggaca aagtgatctg
     5821 ggggccaaac tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa
     5881 cttcgaggcc atgcaaaagg agatgtacgg gcagtttgaa aacaccttca tgatgtacct
     5941 gccgcgcctg tgcgaacact gcctcaatcc cagctgcgtg gcgacctgcc caagcggcgc
     6001 tatctacaaa cgcgaagaag acggtattgt gctgattgat caggataaat gccgtggctg
     6061 gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa
     6121 gtcagaaaaa tgcatcttct gttatccgcg aattgagtca ggccaaccaa ccgtatgctc
     6181 agaaacctgc gtgggtcgaa tccgatatct gggcgtgctg ctttacgacg ctgaccgcat
     6241 cgaggaagcg gcgagcaccg agcgcgaagt tgacctctat gaacgccagt gcgaagtgtt
     6301 cctcgatcca cacgatccct cagtgatcga ggaagccctg aaacaaggta ttccacaaaa
     6361 tgtgattgac gctgcccagc gttcgcctgt ctacaaaatg gcgatggact ggaaactggc
     6421 gctaccgttg caccctgaat accgcaccct gccgatggtc tggtacgttc ctccgctgtc
     6481 accgattcag tcttacgctg atgcgggcgg tttgccgaaa agcgaaggcg tgctccccgc
     6541 catcgaaagc ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggtgatac
     6601 cggtccggta ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca
     6661 aaccgtggaa ggcgttactg atactcgtgc catcgacgaa gtaggcctga gcgtcgccca
     6721 ggtcgaagaa atgtatcgct atctcgccat tgccaactat gaagatcgtt ttgttatccc
     6781 gaccagccat cgggaaatgg cgggcgatgc cttcgcagaa cgcaacggct gcggttttac
     6841 ctttggcgac ggttgccacg gttcggacag taaattcaac ctgttcaaca gtagccgtat
     6901 cgatgccatc aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc
     6961 aaagtgatcg gcctgttgat ggagtatccg gacgagctgt tgtgggaatg caaggaggac
     7021 gcgctggcgt tgatccgccg cgacgcgccg atgcttacgg atttcaccca caacctgctt
     7081 aacgcgccgc tgctggataa acaggccgaa tggtgcgaag tgtttgaccg cgggcgcacc
     7141 acgtcgctgc tgctgttcga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg
     7201 gtggacctgc tggcggagta tgaaaaggtc ggcctgcagc tggattgtcg ggaactgccc
     7261 gattatctac cgctgtatct ggagtattta agcgtactgc ctgacgatca ggcgaaagaa
     7321 ggattgctca atgttgcgcc gatcctcgcc ctgcttggcg gacgcttaaa acaacgcgag
     7381 gcaccctggt cttcgttgtt tgacgccctg ctgcaactgg caggaagctc cctttcaagt
     7441 gacagtgtca caaaacagat tcgcagtgag gagcgtgatg acacccccca ggcgctggat
     7501 gcggtgtggg aagaggaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc
     7561 tcgccgctta atcaatatca gcgacgcttt agccaggatg tcgcgccgca atatgtcgac
     7621 atcagtgcgg gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc
     7681 cgtacatttg tgcgacggtg tttttcctcg gcagctggct gcgctacgac tatggccagt
     7741 acacctggcg cgcctcctca agtcaaatgc tcgataaacg cgggatggtg atatggtcga
     7801 atctgttcca tatcggcatt ttggggatct tcttcgggca cctgttcggc atgttaacgc
     7861 cgcactggat gtacgcgtgg tttttgccag tggcagcgaa acaactaatg gcgatggtgc
     7921 tcggcggtat ttgcggtgtg ttgacgttga ttggcggcgc aggactgctg tggcgcaggc
     7981 tgacaaatca gcgcgtgcgt gccacttcca ctacgccgga tatcatcatc atgagcattt
     8041 tgctggtcca gtgtctgttg gggctaagca ccataccgtt ttcagcacag tatcctgatg
     8101 gtagcgaaat gatgaagctg gtgggttggg cgcaaagcat tgtcactttc cgtggtggat
     8161 cgtcagagat gcttaacggt gtagcgttcg ttttccgcct gcatctggtg ttgggaatga
     8221 ccatcttcct gctcttcccg ttcacccgac tggtgcatgt gtggagcgcg ccgtttgagt
     8281 acttcacccg ccggtatcag attgtgcgct cgcggcgata attttcaccc tggccggata
     8341 gcccttcgct ctccggccac ttgcaggcta tgatagttct ccagtcacag gagaacgtta
     8401 atgaaaccgc aggtttacca cgtcgatgct tttacctctc aaccatttcg cggcaattct
     8461 gccggtgttg ttttccccgc cgataatctt agcgaagcgc aaatgcagct tatcgcccgc
     8521 gaattaggtc attcggaaac cgcttttctg ctgcacagcg acgacagcga tgtgcgcatc
     8581 cgctacttta cgccaacagt tgaagtgcct atttgcggtc acgcgacagt agctgcgcac
     8641 tatgtacgtg cgaaggcgtt aggtttagga aattgcacga tctggcaaac gtcactggca
     8701 ggaaaacatc gcgtgactat cgaaaagcac aacgatgatt atcgtatttc gctggaacaa
     8761 ggtacgccgg gctttgagcc accgctggaa ggtaaaacac gtgcggcgat catcaacgcg
     8821 cttcatctca ctgaagacga tattctgcca ggcttgccaa ttcaggtggc aaccacagga
     8881 cactcaaaag tgatgatccc actgaaaccg gaagtggata tcgacgccct ttcgcctgac
     8941 cttaatgcgc tgaccgctat cagcaaacag attggctgca atggtttctt cccgttccag
     9001 atccgcccag gcaaaaacga aaccgatggt cgcatgttct cgcctgcgat tggtattgtg
     9061 gaagatccgg tgactggcaa tgccaatggc ccgatgggtg catggttggt acatcacaac
     9121 gtattgcccc acgatggcaa cgtgttgcgt gttaaaggcc atcaggggcg cgcattgggg
     9181 cgtgacggta tgattgaagt gacggtgaca attcgtgata accaaccgga aaaagtcacc
     9241 atttctggcg cggcggtgat tttgtttcat gccgaatggg caattaaact ctgaaaccgt
     9301 tgcctgatgc gcttcgcctg tcaggtctac aaccgagcta atgtatgccg gataaggcgt
     9361 ttacgccgca tccggcaacc tgacataaat tattttcccg cctccgggtg cgtatcaaac
     9421 gccgccatca ccagcgctaa ctcatccaca gtaaagccat gtttcgcatc atccacgccc
     9481 agaccaaact gttcttgcat cacagcatat aacgacgcca catccggtag atttcgctgc
     9541 tccaccgcgt gctcattttc ataatgggta aaatgaaaat tggtcagtgt cagcttgccg
     9601 ccgtccggta aatggcggca catcagcaaa tgatggcgaa aatgcgactg cggccagtgc
     9661 gccgaccaga aattgcccat cacataatcg ctttgttgct gctcgcagag atcaaaacgg
     9721 tacatcgact gccagtgctg atgatgatta aactgcaaca cccaatcatc accctcctgt
     9781 aacaaccgat actctccgtg tggcgtgatc tgcacgagat cgggaactaa acgaatcggc
     9841 gcggttagcg tctgtccacc gaaaccgaca tcagcaatcc atttttcccc ttccagttcc
     9901 accaacaaca aacgatgggt gcgcggcggt aatgctggcg gatttgataa cactacgcgc
     9961 cctaacaagc tgcgaacgtt aaaccccagc tcgcgtaaca cccgctcaaa cacgccattc
    10021 tgctcaaaac agtaaccacc acgacgggct gtcaccagtt tctcttccag cgattgatca
    10081 tcaagctgta tttccctcgg cagcaaaacg tcgaggtttt caaacggaat ggtgcaattg
    10141 tgtttcaggt gcaatgcacg aagcgtatca atattgaccg cagcagctcc cgaccagtta
    10201 atacgggcaa aatagtgatt cagaatgggc gtcatcaggc gtttccctta gtggttttct
    10261 caacttataa cctggaatag acgccgcacc ggctattttg tgcgatcgcg gacatgcatc
    10321 acgttcgcaa atcgccaatc ccgttaattt cgttacttat tgcgttacgg actacgctta
    10381 aaaaaacctt cttcgcgagg cgataccatg agccgattca tccccataga attacaccat
    10441 gccagccgtc tgttaaatca tggcccaact gtcttgatca ccagtttcga tgagcaatcc
    10501 cagcggcgta acattatggc tgcagcctgg tcaatgccgg tggagtttga acctccacgc
    10561 gtggcgattg tggtagataa atcgacatgg accagggagt tgattgaaca taacggtaaa
    10621 tttggcatcg ttatcccggg cgttgcagca actaactgga cgtgggcagt gggaagtgtg
    10681 tcggggcgtg aagaagataa atttaattgc tatggcattc cggttgtgag aggcccggta
    10741 tttggtttgc ctctggtcga ggaaaaatgt ctggcgtgga tggagtgtcg cctgctacct
    10801 gcgacttctg cgcaagaaca atacgacaca ctgtttggcg aggtagtatc agcagcggca
    10861 gacgcacggg tatttgtcga aggccgctgg cagtttgatg atgataagct caatacgttg
    10921 catcatttag gtgctggcac gtttgttact agcggcaagc gtgttacggc gggttaagca
    10981 ttacatgctg taaccaggtt tctttatcaa agcctccatt tggggcgcga tttcggcatc
    11041 ccagatagct tgccatgatt ctggttgaat ctgctgtaga gaaatgctta tcgagctatc
    11101 tttactgttc agatggcgaa taataacgtc ggtaatatct gcagcaagtg ctgctttttg
    11161 ttgttcgtcc agttcacgcg gaaaacattt aatgtcgatg tgcggcatga ttgtgctcct
    11221 tttgaaagat gtcagtaaaa ttaacacaca ttgtgaatgt cgtatgtccg gtgatgcgtt
    11281 tttacgacaa taatgccagg gcaagattac gaaagccttc tccccgcaag gactgacgcc
    11341 aggtagtttc tgtccatggc tgcttttcgc atcttacgtc tt
//
LOCUS       DAZFFG010000132        11265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFFG010000132 DAZFFG010000000
VERSION     DAZFFG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.132"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..2920
                     /locus_tag="ACHJ7J_004155"
     CDS             <1..2920
                     /locus_tag="ACHJ7J_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5002320.1"
                     /translation="DLKFHGGGNIELTDNLNSGSGGLIFDEGQYYSISGKDKTYKGAG
                     IDIGKDTVVDWSVKGEANDNLHKTGSGTLNVNVAQGNNLKMGDGTVVLNAAKAFNAIY
                     VASGRGTVKLGQADALDKNSDYRGIYFTSRGGTLDLNGFSQSFKKIAATDVGTIITNT
                     SDKTATLSLQNPSRYVYHGSITGNTNIEHTGTQKSADSSLIIDGNIDTHNDISIRNSQ
                     LRLQGHATTHAIFREGPRHCYVPGVLCDKDYVADFAKLESEANKKNNSAYKTNNQVAS
                     FDQPDWETRHFRFKTLNLENSEFTTARNSVAEGDIVASNSTLKLGGDVPVFIDMYDGI
                     NITGNGFGFRQDVREGRSADDGSSSYTGNITLQKGSTLDINNRFTGGIEAHDSKVNVT
                     SPDALLQNSGVFMNSTLSVRDGGHLMAQKGLYSDGRVQIGKNGTLSLSGTPENGADNT
                     WMPVLTYMTEGYDLTGDNATLNISQQAHVSGDVHATSSSSIRIGSENPGSVSSSVSPV
                     LAAGLFSGYNAAYYGAITGGKGNVSMNNGLWQLTGDSDINSLTTRNSRVQSEEKGAFR
                     TLTVNTLDATGSDFVLRTDLKNADKIHVTKKASGSDNTLNVSFMKNPSPGQSLNIPLV
                     SAPAGTSGDIFKAGTRVTGFSRVTPTLHVDTTGGSTKWILDGFRTEADKAAAAKADSF
                     MNAGYKNFMTEVNNLNKRMGELRDTNGDAGAWARIMNGAGSADGGYSDNYTHVQIGFD
                     KKHALDGVDLFTGVTMTYTDSSADSDAFSGKTKSVGGGLYASALFNSGAYIDLIGKYI
                     HHDNDYTGNFAGLGTKHYGTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFN
                     WKDGEMDLSMKNKDFSPLIGRTGIELGKTFRGKDWSVTARAGTSWQFDLLNNGETVLR
                     DASGEKRIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAINANFRYMF
                     "
     gene            3133..3405
                     /locus_tag="ACHJ7J_004156"
     CDS             3133..3405
                     /locus_tag="ACHJ7J_004156"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5002321.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3632..3928
                     /gene="ghoS"
                     /locus_tag="ACHJ7J_004157"
     CDS             3632..3928
                     /gene="ghoS"
                     /locus_tag="ACHJ7J_004157"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5002322.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3956..4129
                     /gene="ghoT"
                     /locus_tag="ACHJ7J_004158"
     CDS             3956..4129
                     /gene="ghoT"
                     /locus_tag="ACHJ7J_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO5002323.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4248..5765)
                     /gene="lysS"
                     /locus_tag="ACHJ7J_004159"
     CDS             complement(4248..5765)
                     /gene="lysS"
                     /locus_tag="ACHJ7J_004159"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5002324.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(6002..7459)
                     /gene="dtpC"
                     /locus_tag="ACHJ7J_004160"
     CDS             complement(6002..7459)
                     /gene="dtpC"
                     /locus_tag="ACHJ7J_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO5002325.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7518..9665)
                     /gene="cadA"
                     /locus_tag="ACHJ7J_004161"
     CDS             complement(7518..9665)
                     /gene="cadA"
                     /locus_tag="ACHJ7J_004161"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO5002326.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9745..11079)
                     /gene="cadB"
                     /locus_tag="ACHJ7J_004162"
     CDS             complement(9745..11079)
                     /gene="cadB"
                     /locus_tag="ACHJ7J_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO5002327.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         3153 a         2619 c         2724 g         2769 t
ORIGIN      
        1 agatttaaaa tttcatggtg gcgggaacat tgaactcacc gataacctga attcaggatc
       61 tggaggattg atttttgatg agggacaata ttattcaatc tccgggaaag ataaaacata
      121 taaaggtgcg ggtattgata tcggaaaaga tacggttgtc gactggtcgg taaaagggga
      181 ggcaaacgat aacctgcaca aaacaggatc cggtacactg aacgttaatg tggcccaggg
      241 gaataacctg aaaatgggtg acggtactgt tgttcttaat gcagcaaagg cctttaatgc
      301 tatctatgtt gccagtggtc gcggaacggt caaactaggg caggccgatg cgctggataa
      361 aaatagtgat tacagaggta tttattttac cagtcgtggg gggactctgg atttaaacgg
      421 gttcagccag tcgtttaaga agattgcggc aactgatgtt ggtaccatta tcaccaatac
      481 ttctgataaa acagcgacac tttccctgca aaacccctcc cgttatgtct atcacggtag
      541 tatcacggga aatacgaata tcgaacatac tgggacacag aaaagtgctg acagtagcct
      601 gattattgat ggaaacattg atacgcacaa tgatatctcc atacggaatt cccagcttcg
      661 gttacagggc catgccacaa cacatgcaat attccgcgag gggcctcggc actgctatgt
      721 ccccggagtt ctttgtgaca aagattatgt tgctgatttt gccaaactgg aaagtgaggc
      781 aaataagaaa aataacagtg cctataaaac aaataaccag gtggcttctt ttgatcaacc
      841 tgactgggaa acccggcatt tccgatttaa gactctgaat ctggaaaact cagaattcac
      901 aactgcacgt aactcagttg ctgagggtga tattgtcgca tcgaattcaa cgctgaaact
      961 ggggggcgac gttccggtat tcattgatat gtatgatggc atcaatatca ccggtaatgg
     1021 ttttggcttc cgccaggacg ttcgtgaagg acgctctgca gatgatggca gtagcagcta
     1081 tacgggcaat attacactgc aaaaaggctc cacgctggac atcaataacc ggttcactgg
     1141 cgggattgaa gcccatgaca gtaaggtaaa cgtcacctca ccggatgccc ttctgcaaaa
     1201 cagtggtgtc ttcatgaact ccaccctctc tgtccgtgac ggcggtcatc tgatggcaca
     1261 aaaagggctc tacagtgatg gtcgggttca gattgggaag aatggtacgc tttccctgag
     1321 cggcacgccg gagaatggcg ctgataatac ctggatgcct gttctgacat acatgacaga
     1381 aggctatgat ttaaccggtg ataacgccac gctgaacatc agccagcagg cccatgtttc
     1441 cggggatgtt catgctacca gttcatccag cattcgtatt ggctccgaaa acccgggctc
     1501 agtttcctct tctgtctccc ctgttctggc tgccgggttg ttcagcggat ataacgcggc
     1561 gtactacggt gccatcaccg gcggtaaggg gaacgtcagt atgaataatg gcctgtggca
     1621 gctgaccgga gattccgata tcaacagtct gacgacccgt aacagccggg ttcagtctga
     1681 agaaaaggga gccttccgta ccctgacggt taatacactt gatgccacgg gcagtgattt
     1741 tgtcctgcgc actgacctga aaaatgcaga taaaattcat gtgaccaaaa aagccagcgg
     1801 ctcagataac accctgaacg tcagttttat gaagaacccg tctccgggac agtccctgaa
     1861 catcccgctg gtcagtgcgc cggccggaac atcaggggat atctttaagg cgggcacccg
     1921 ggtgactggc ttcagtcgtg tgacgccgac gctgcatgtc gacaccacgg gtggcagtac
     1981 gaagtggatt ctggatggtt tcaggacgga agccgataaa gcggctgcag cgaaggcgga
     2041 cagtttcatg aatgccggct acaaaaactt tatgacggaa gttaacaacc tgaacaaacg
     2101 tatgggtgaa ctgcgtgaca cgaacggtga tgccggtgcc tgggcccgta tcatgaacgg
     2161 cgccggttca gccgatggcg gatacagtga taactacacc cacgttcaga tcggttttga
     2221 caaaaaacat gcgctggacg gtgttgacct gttcaccggt gtcacgatga cctataccga
     2281 cagcagtgca gacagtgatg cgttcagcgg gaagacaaaa tccgtggggg gcggtctgta
     2341 tgcttcagca ctgtttaact ccggtgccta tatcgatttg attggtaaat atattcacca
     2401 tgacaatgac tacacaggca actttgccgg tctgggtacg aagcactacg gaacccactc
     2461 ctggtatgct ggagcggaaa cgggttaccg ttatcacctg acggaagaca catttattga
     2521 gcctcaggcc gaactggttt acggcgcagt gtccgggaaa acattcaact ggaaagacgg
     2581 tgagatggat ctaagtatga agaacaagga ttttagcccg ttgattggca gaacagggat
     2641 tgagctgggc aaaaccttca ggggtaagga ctggagtgtg acagcccgtg ccggaaccag
     2701 ttggcagttt gacctgctga ataatggcga gacggttctt cgtgatgcct ccggagaaaa
     2761 acggatcaaa ggggagaaag acagcaggat gttgttcaat gttggcatga acgcacagat
     2821 aaaggacaac atgcgctttg gtctggagtt tgagaaatcc gcctttggta aatacaacgt
     2881 ggataacgca ataaacgcga atttccggta tatgttctga caaggagagg cccctgaaga
     2941 ggggcctgtg tgatgttact cccgggttaa tgcagctagt aaaggtatgg ctctgactac
     3001 cctggaaagt tgtgcccatc gcatcactac aaattctggc aattgcgtac gtggtaacgg
     3061 caagttattt aacctcaaac gtacgtgccc gagtggtgcg ctgaaatacc gtcataaata
     3121 agcgagggta aaatggaaat acgcgaaggc cacaataaat tttacattaa tgacgaacaa
     3181 ggcaagcaaa tcgctgaaat tgtctttgtg ccgaccggag agaatttagc gattatcgaa
     3241 cataccgatg tcgatgaaag cctgaaaggg caagggatcg gtaaacagct ggttgcgaaa
     3301 gtcgtggaaa aaatgcgtcg ggaaaaacga aaaattatcc cattatgtcc atttgcgaaa
     3361 cacgaatttg ataaaacgcg ggagtatgat gatattcgca gttgatggaa gagtacagat
     3421 tcacgatatt tttcgtgctc tccgcggtgt gatgcaggag agcatctgaa gggtaggggg
     3481 atgcacaaag aatgggcaga gagcgcgttt ttttgtccca agtcatcccc tttactgagc
     3541 aaaaaaaaga atatctccta tatgagaatc atcaatcggg gttaataagt tttgcgtccc
     3601 cagagcgttc aatattgata ggagtcatat tatggaaggt aaaaacaagt tcaatactta
     3661 tgttgtttct tttgattatc catcatctta ttcctcagtg ttcttaaggt taagatcatt
     3721 gatgtatgat atgaatttct cctctatcgt ggctgatgaa tatgggatac cacgacaatt
     3781 gaatgaaaac tccttcgcaa taacgacatc gttagccgca agtgaaatcg aagatttaat
     3841 caggcttaaa tgcttagact taccggatat tgactttgac ctcaacatta tgacagttga
     3901 tgactatttc cgtcagtttt acaaatagcc aggaaggata agaagaacat aaaaaatggc
     3961 actattctct aaaatattaa ttttttatgt gattggtgtg aacatatcct ttgtcattat
     4021 ctggtttatc tcacatgaga aaacacatat tcgtttactt agtgcattcc tggtcggaat
     4081 aacctggcca atgagtctgc ctgtggcatt acttttttct ctcttttagg agctgtagtt
     4141 gagtggaccc gctggttatc gtgaaacgaa gcgtctctcc tcaaaactgg atttgcggaa
     4201 cgatgaacga agcagtcgcc tgactgcttc gttcattaaa gtgaaattta tttctgtggg
     4261 cgcatcgccg ggaagagaat aacgtcacga atagtatgac tgtttgtaaa cagcataatc
     4321 attcggtcga taccaatacc cagaccagcg gttggcggca gaccatattc cagcgcagtc
     4381 acgtaatctt cgtcatagaa catggcttcg tcgtcacctg cggctttagc gttaacctgt
     4441 tcctggaaac gttcagcctg atcttctgcg tcgtttaatt cgctaaaacc attaccgatt
     4501 tcacgaccac cgatgaagaa ttcaaaacgg tcggtgattt ccgggttaac atcattacgg
     4561 cgcgccagcg gggacacttc tgccggatat tccgtaataa aggttggctg aatcagatgt
     4621 gcttctgcca cttcatcaaa gatctccgtg acaatacgtc ccagccccca gcttttctct
     4681 accgtaattc cgatagattc agctaatgct ttagcagcat caaaattatc caggtcggcc
     4741 atatcggttt ctggacgata ttttttgatt gcttcgcgca tggtgagttt ttcaaacggt
     4801 ttgccgaaat caaacacatg ctcgccataa gtgactttag tggtaccaag aacctcttgt
     4861 gccagggtgc ggaacaggga ctctgtcagt tcaatcaaat cgtggtaatc cgcatacgcc
     4921 atgtagagtt ccatcattgt gaactcagga ttatggcgaa cagaaatacc ttcattacgg
     4981 aagttacggt tgatttcgaa tacccgttca aaaccgccta caaccagacg tttcagatac
     5041 agctccggcg cgatacgcag atacatatct aaatccagag cattatgatg ggtaataaac
     5101 gggcgagcag atgccccacc tggaattacc tgcatcatcg gggtttctac ttccataaag
     5161 ccgcgcgcga ccatgaactg acggatagcg gccagaattt ttgaacggac aacaaacgtt
     5221 tgacgggatt tatcgttagc gatgaggtcc agataacgtt gacgataacg aacttcctga
     5281 tcctgcagac catggaattt atctggtaaa ggacgtagtg ctttagtcag caggcgcagc
     5341 tcagtacagt gaatggaaag ctcacccgtt tgcgtcttaa acagcgtacc gcgggcaccg
     5401 ataatgtcac ccagatccca ttttttaaac tgatcgttat aaacaccttc tggcaggcta
     5461 tctcgtgcga cgtacagttg aatacggcca ccgacatcct gcaacgttac aaaggaggct
     5521 ttccccatga tacgacgggt catcattcgg ccagcaaccg agacttcaat gtttaaggat
     5581 tccagttcct ggttatcctt cgcatcaaac tcttcgtgca actggtcaga ggtatggtcg
     5641 cggcgaaaat cattgggaaa cgccacacct tgctgacgta gtgccgccag tttttcgcgg
     5701 cgatttctca gttcgtcgtt aaaatcaata gcctcattgg ctccccgtgt ttcttgttca
     5761 gacattttgg ttcctctaaa tccagctttc aatttagcgt agataaagag acagatcggt
     5821 acactgtatt atctgcctcg actattagta actcagttta tcttatcgaa actaacgctt
     5881 acctcaagtt gatatcacta ataaataacc atcaaaatgg tttaaatgac ttattaatat
     5941 cgattttcta gtgttataaa ctgaaagtta atttattcat aactaattgt ttttacgaca
     6001 gttaatcgtt gctctcctgt atcatattcg ttggcgtgct aaacagaaaa cgggtagcaa
     6061 aggccaatac gacgatgatc gcgacacaag ccaacgtcca ctctcccatc tgagaaaaga
     6121 atcgctggta agctgcaatt gccataccgc taatttgcga ctctgtcgtc tgctgtgcca
     6181 ctacgcctgc cagccagttg gcgaccgcgc ctgttgccag catataaata ccggttaata
     6241 cgccagacat tttcagacgc gtgatttgcg caatcgccac cgggtcaata aagagttcgg
     6301 caaagcccat tagcgccagc ccggatatca tcacgcccat tgacgcttga ccgccagccg
     6361 ctgcatgtcg ggcatcaaat gccagcaaca taaagccaca agccatcaga agtaagccaa
     6421 aggcaaactt cagccagacg cgcaatgttg agttgccgcg gctttctggc gatgccagcc
     6481 aggccagtac aaccccagcg agcatcaccg caattgcatt caccgactgg aatagtgctg
     6541 taggtacttc aatattgaat gcctgacgat tcacaaagcg atcgataaac aagctgatgg
     6601 tactgccgcc ctgttgtgcc agtacccaga acaatgtccc gacaaacatc aacaatacaa
     6661 tttgccaaag agcacggcgg tgttcgggga atttgatcat catgcgggca atgatttgtg
     6721 cggcgataag gcaaacgatc gccagcaaat atccggacca gtcgttctcc agcagcagag
     6781 taaaaaatac tggggctaaa cagagcatca ccactaacca gctccatact ggtaaggcaa
     6841 atttgacact agtgagcgct tttttatcca tactacgcgt ggactggaaa tgacgatgac
     6901 cgcttaagaa aatcaacaaa ccgataaaca tgccgccacc cgcaagggca aagccaacat
     6961 gccatccata ccactgagca gccaggccgc aggcgattgg ggcggcgata gaaccgatat
     7021 tgcccgcagc atacagcagc gaaaaaccgc catcacgtcg atgatcgttc tcgtcgtaga
     7081 gctcgccaag caaacagctg atgtttgatt tgaataaacc gtagccacaa ataatgattg
     7141 ccagcgccag atacaggcta aaggttgaat ttgtatcaat gcccagcacc acatggccaa
     7201 gagtcattaa cagcgcgccg gcaatcactg cagtgcggtt gccgagcagg cggtcggcaa
     7261 gccagccgcc gagaataggg gtaacgtaaa ccagagaagc atatgcgctg aacaggctga
     7321 tggcatggtt atcatcaaaa ccaagctgat gggtgagata gagaatgagc aaggcacgca
     7381 tgccgtaaaa actgaagtac tcccagattt ggatcgccac gatatagtat atcgcgcgcg
     7441 gctgtgaggg tgttttcatg tgttctcctt atgagcaaaa aagggaagtg gcaagccact
     7501 tcccttgtac gagctaatta ttttttgctt tcttctttca ataccttaac ggtatagcgg
     7561 ccatcagcct gacggtatgc accgtgaata tcggtttcaa agcccggata gtgagcgccg
     7621 atttcacaca gcatctgcag gaactccaga accggacggc tttcttcggt gatcatttca
     7681 cccggcatta ccagaggaac tcccggcgga tacggaagga tcatattggc gttaatacga
     7741 cctaccattt cgtcgaggta aacttcttcg gtcataccgt gcagctcttt ctggaacgca
     7801 gcatacggag tcattaccat tgtcggcagc acttcaaatg cgcgatacat cagatccggc
     7861 agattgtggt gaacaatcag tttgtggata ttctgagcca gttcctgaat acgcatgttt
     7921 tcatagaatt caggatcttc acgatacaga gacggcagca tgtttttcac acgcaggttc
     7981 aggtcgaacg cacgtttgaa gtcagtcaga gcacgcagca ggctcagtgc tttggtctta
     8041 tcgataccga tgctgaacag gaacagcagg ttatacggac cggttttctc aacaacgatg
     8101 ccatgttcgt cgaggtattt cgccacgatg ctggccggaa taccaaagtc gctcatggtg
     8161 ccgtcttttt ccatccccgg agtcagcagg gtgactttga tcgggtcaag atacatgtgc
     8221 tcgttatcga tgtttttgaa gccgtgccag gtgctgtcag aacgcagcgg ccagcattca
     8281 gtcgtatcga tatgatccgg ctgccataca tcaaagaacc agccatcaga ttccgttctc
     8341 agacgtttga tctctttacg gaatttgatc gcacgttcaa tggaaccgtt gatcagacgc
     8401 ttacctgcat tgcctttcat catcgccgca gcggtttcag tggacgccac gataccgtag
     8461 tgcggagaag tggtggtgtg catcatgtag gcttcgttaa aggtttcttc gtttacgtca
     8521 cctttaacgt ggatcatgga agcctgagag aacgccgcca gcagtttgtg agtggactgg
     8581 gtttcgtaaa tcactttccc ttcgacacgg ccaccgctca taccgcattt accttcgtaa
     8641 atcggtgaga agttggtgta aggcacccac gcggagtcaa agtggatgga tttcacatcc
     8701 agtgttttct tgatgaagtc ggtgttgtac agcagaccat cataggtaga gttggtaatt
     8761 acagcatgta ccggccaggt tgcgtttggt gtttctttca cgcgcttagc aatggtagcg
     8821 tgctggaatt cactctgtgg gataccacca agaataccgt aagcgttacg ggtcgggcgg
     8881 aaatagattg gcgtaacatc gctcatcatc atcaggtggg tcagcgattt gtggcagtta
     8941 cggtcaatca gaatggtgct gcctgccgga gcagagtaca taccaacaat tttgttcgca
     9001 gtggaagtac cgttggtcac catgtagctg cggtctgcgt taaagacgcg agcgatatac
     9061 tgttctgctt ctttgtgtgg accactgtga tccagcagag aacccagttc agatactgaa
     9121 atggaaatat cagatttcat ggtattcgga ccaaagaaat catagaacag gctacctacc
     9181 gggcttttct ggaatgcagt accgcccatg tgaccaggag tacagaaagt atatttacct
     9241 tcacgaacat atttaaacag tgctttagtc agcggaggca gaatagtgtt gatatattcg
     9301 tcagtggtct gcttgatctt attagcaata tcttcagcag cacccagcgc atattcaaag
     9361 aagctaatct gtaaacgcag gtcattcagg cttacatcga gagtggaata cgtattagcg
     9421 aacgcgtaca acggcaggtt ctcgttcatt ttgctaattt cttcgcacag ctcgagatta
     9481 tatttatccc agtcaaaaat aacgccgcac agacgcgcat tgttttcgat cagttttaat
     9541 aagtcgtcac ggtcgttcgg gtaaacaatc tggaagttca gacgttcaag cgcgcgatga
     9601 agttcacgga tgggttcttc tttaaaataa acccccatgt gattcaatat tgcaataacg
     9661 ttcatagtca tatctccagg taaaaaaggc ccctcccaac acatgggaca aaatgaaagg
     9721 aggagcctcg gaaaatactt ttaattaatg tgcgttagac gcggtgtggt tatccattga
     9781 gtggctctgg cgctcgtgca ttttgcgagc gtagaacatc aggataatca ggctgacgat
     9841 gaaggtacct gccagctcga aggagcttgc gcccatcagc gcgatgaagc agaacacgca
     9901 acccagtaca gagcagatca ggctgacaaa gttgcggatg ttaacgcctt caaaacgaat
     9961 caggtcaacg caagagtaga agtacggcag catagtcagc agtactgcga taccggtcag
    10021 ttcaccgaac aggtcagatg ctttaccacc ggcagagttc atcagagtga tgaggatcat
    10081 cagggcagtc attttcactg cagccagcag cagacctttt ttcggaatac cgttgctgtc
    10141 gacttcacca taaactttcg ggaagttacc gtcgttagcg gcacgtacac ctgcctggcc
    10201 taccaacatc atccaggagc ccagagaagt caggcacgca aaggcggtga atgcagaaac
    10261 cagcggcgca gcccagttac cgaggatagt tgaagcactg attgcaaacg gagcaccgga
    10321 agctgccatt acagaagacg gatacatacc ggaaagcacc tgagtcgcag cgatataaac
    10381 aatacctgct aaaccagtac ccagcatggt tgccagcgga acggtacgtt tcgggttttt
    10441 aaccatacca gtacttactg ctgcggattc aacacccacg aaggcccaca ggcagagcag
    10501 aatactttta atgatcgcat gaccatcagt ggtatccgca gtattccagt tagctgcata
    10561 agttgccgca tcaaaccaat gccagccaac aatagcagtc atcaccacag gaataagaac
    10621 cagcaccaga ccaatagtgg ttaaacggct tacccaagta ccgccgagca tatttacaaa
    10681 ggtaaatacc cagacgatag caatacaggc gatacccgcc ggaacaggat catttaatac
    10741 tgggaagaag gtggaaagat aagatacagc ggtaatacca atcgccaggt taccaatcca
    10801 gttagcatgg taataaagaa cacctgtctg aaaaccaaat gcaggagaaa tttctccggc
    10861 ataagcaatt gggccacctt gttgcgggtt ttttgttgcc agtcgggcat atacatacgc
    10921 cagcgacatt gcaccaataa tagagataat ccaaccccag atagcaatac caccgatact
    10981 tgctaggttc gcaggtaata atgcaatacc gctccccatc atattaccgg caacaacacc
    11041 ggtacaggca aatagcccga tcttcttggc agaactcatg ctcttctcct aatttcattt
    11101 ttgaatttgg agtccgggtc atgatgtata actatttcct gaccagacca aactggcgct
    11161 aagattactc acgaaaaaag gattaatcct aaagattagg tgaaataaca caaaagtttc
    11221 tgtaagtgag aacttgaggt tttttattaa cacatcagga tcgca
//
LOCUS       DAZFFG010000133        10986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFFG010000133 DAZFFG010000000
VERSION     DAZFFG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.133"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            90..1574
                     /locus_tag="ACHJ7J_004163"
     CDS             90..1574
                     /locus_tag="ACHJ7J_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5002328.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(1896..2498)
                     /locus_tag="ACHJ7J_004164"
     CDS             complement(1896..2498)
                     /locus_tag="ACHJ7J_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002329.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(2592..2798)
                     /locus_tag="ACHJ7J_004165"
     CDS             complement(2592..2798)
                     /locus_tag="ACHJ7J_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002330.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3553..3684
                     /locus_tag="ACHJ7J_004166"
     CDS             3553..3684
                     /locus_tag="ACHJ7J_004166"
                     /inference="COORDINATES: protein motif:HMM:NF017162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulating family protein"
                     /protein_id="HFO5002331.1"
                     /translation="MRYRLTPVDLEARNSAVAHRLTELTANFTSACWLPSGNMSPEY"
     gene            complement(4024..4224)
                     /locus_tag="ACHJ7J_004167"
     CDS             complement(4024..4224)
                     /locus_tag="ACHJ7J_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002332.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            4424..4993
                     /locus_tag="ACHJ7J_004168"
     CDS             4424..4993
                     /locus_tag="ACHJ7J_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5002333.1"
                     /translation="MTYKYNPFWQQRIRETVLHALNVHPRLTVLRVDLRLPDVPAATD
                     AAVISRFINALKARINAYQKRKRREGKRVHPTTLHYAWAREFGVFRGKKHYHLLLLVN
                     RDTWCRAGDYRDPGSLAGMIEQAWCSALGVDAGCYATLVHFPVRPAVWLERDDDAGLR
                     DMLKRADYLAKEYTKVNGTGERNFGCSRS"
     gene            5253..5636
                     /locus_tag="ACHJ7J_004169"
     CDS             5253..5636
                     /locus_tag="ACHJ7J_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5002334.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            5633..6058
                     /locus_tag="ACHJ7J_004170"
     CDS             5633..6058
                     /locus_tag="ACHJ7J_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5002335.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSAQQKAACVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRATCTKSVYSYQPDLYQDCGGAST"
     gene            6307..6504
                     /locus_tag="ACHJ7J_004171"
     CDS             6307..6504
                     /locus_tag="ACHJ7J_004171"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5002336.1"
                     /translation="MHQDDFCIVNPGGCIIHFPANGRYILDIHDPDFSEQYQRLLERL
                     DYLKKPGTKASGQRNSGYSGF"
     gene            6532..7098
                     /locus_tag="ACHJ7J_004172"
     CDS             6532..7098
                     /locus_tag="ACHJ7J_004172"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5002337.1"
                     /translation="MYTESNVNLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GLNKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQVWGKVWGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            7145..7417
                     /locus_tag="ACHJ7J_004173"
                     /pseudo
     CDS             7145..7417
                     /locus_tag="ACHJ7J_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7485..7718
                     /locus_tag="ACHJ7J_004174"
     CDS             7485..7718
                     /locus_tag="ACHJ7J_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002338.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYRLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESREAAA"
     gene            8364..9482
                     /locus_tag="ACHJ7J_004175"
     CDS             8364..9482
                     /locus_tag="ACHJ7J_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO5002339.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAEEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGK"
     gene            complement(9631..10905)
                     /locus_tag="ACHJ7J_004176"
     CDS             complement(9631..10905)
                     /locus_tag="ACHJ7J_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO5002340.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKAVQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHYFVAGYQRRIRLYFPQNPTSVPLGLLVLPDAE
                     IWFDRMDITRALDMAITTGHITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTILAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENDTSWVSEQILSAPPKDVNIQLGVGSLEGTTVSHVQRLHQSLIAAGLESNLTV
                     YAGGHDYAWWRGAIIDALANYNCRKISDNNFV"
BASE COUNT         2868 a         2441 c         2737 g         2940 t
ORIGIN      
        1 aatttttacc ttatattgat ttaactgcta tttttgctat taatagccag aattgcagtg
       61 cttttatatc tgtgtgtagt gaggttacta tgtcattcgg tcagccttgt gatgagtttc
      121 ccctgagttc gctgcctcca ctcattcgtg atgccgttat tgaagctcag caaattacgc
      181 aggctcccct tgggcttgtg gcagcgtctg ccctgggcgc tgtttcactt gtctgccaga
      241 atctgattga tgtctgcaga ctgaatacac tgcgggggcc ggtctctctt tttttcctga
      301 cgcttgccga gtccggagaa cgaaaaaccg cagtggacaa attattaatg aagcccctgt
      361 accagcagga gatgcagtta tattccagat acaaaagtga actggctgtc tggaaaaaca
      421 aggaagagct tttaaaggct cagaaaaaag cattgttgtc aaaactgaat aaagaactcc
      481 gcaaaggcgc ggatgagtca gaaaccctca gacaactgga agtacttcag aaaaacagtg
      541 cggaagaacc tgtgaggtat aaattcattt ttaatgatgc cacgactgcg gcaattaaga
      601 atcagctttg tggtaaatgg cgttcagtgg gtattatgtc agatgaagcc ggtattatat
      661 ttgatggata taccctgtct gagttgcctt ttattaacaa aatgtgggat ggctctgtat
      721 tgtctgtgga cagaaagaat gaacccgaac agatgattga aaatgccaga atgacgttat
      781 ccctcatggt gcaacccggt ctttttgacc gatatatgga gcgaaaaggt tctgttgcca
      841 gggacagtgg ttttcttgcc cgttgtctta tcagtaaacc tgcgaccacg caggggaaac
      901 gctttattaa cggagctgtg attccgggag gatcattaac ggcatttcat gaacgattaa
      961 tggaactggc cagaggcagt attgaaaaat ccagtgagga tgaacggtat tgtctgcatt
     1021 tttcgcctga agcgcaaaaa atatttattg agcactataa tgttctggag caggatttat
     1081 ccccttccgg acccttaagc ccgtttcggg gacatgtttc aaaaaaaaca gaaaacattg
     1141 caagaattgc agcgttattt cagtatttca gctatggtga agggaaaatc tctgccgata
     1201 tcatgacgtc tgctgtcgta ataagttcgt ggtacacaga tgaatataaa aaattatttg
     1261 cgttacctga tgaatctgaa ctgcagcaga aggacgccga ggagctgttc gactggctca
     1321 ttgaggagtg ccgcggagaa tgcccgcccc gtgtcaggaa aaattatatt ctccagtgtg
     1381 gcccgggccg gttcagaaac aggaaaaaac tgaacgcatt gctgaacatt cttgaatctc
     1441 agttcaggct ttctgtcgtg ccggaaggaa aaaccatgta tgttctgctt ccccagatag
     1501 ccagtttaaa gttgtctgat gtctctggta tttttacctc gggttatcac tacaataagc
     1561 tgagagcgaa gtaaccggtg agcagttttt ctccattatg cttcagactt agatgattga
     1621 ttattatggg gatttcatca ccgatattca gatgtatgaa tatcggtgat gaactaaagc
     1681 tcaggagctg cgtggtggtg atgaatcaac aggcatataa ctgatacccg ccggtcagaa
     1741 gttccagcct gcgactttcc tgtctgcgtg cccgcagtcc ggcgttcagg tgcagcgtgt
     1801 gacagaacgc acggagagcg aaacggcagg cagacaggcc gttcagtcag ccctggcacc
     1861 ctgcccggat accagcttcc ctgaaacaac tggcatcagg tgcgcttatc gtcaaccgtt
     1921 gtgcggtatg ccaggagcac gtactgatcc atcagatccg tcagtgctgc gctctgcttc
     1981 tgacggttga tgatccattc ctccagctct ttttctgatt tccgtttcag attctccgtc
     2041 cagtcgtcaa ggcagtttcc ggtcattaaa tcaagccagc aaagccagac cagtttgagg
     2101 cgcaactccg tgccaccaaa ctgacggaag aggctgatca gtttatttgc cagcgaacgc
     2161 tgcctcagac gttcacattc agacaggaga atgccttcca gcatcggatg atgaaccagc
     2221 agatgttcac tggtgcataa attcactatc agatcatcat ccaggggcgg cgtggtccgt
     2281 ttgtccggac cataaatcag ccggtgcaga ctctggggta cgatggcaat ctccggtttt
     2341 tcaccattga atgacaccca cccgggcgtc agattatgga agtagtagat attcggatga
     2401 cacacgcccc aggatacggc gtgccgggtg aagccggtca gccatttctg gtagctttcg
     2461 tggagcagct cgtaacgggt ctgatactgt ggtttcattt tcatattcct cacgggggat
     2521 aacaggcagc agagcgccgc cggcaggccg gagattgcgt ctgctgtggg cgaagggtta
     2581 gtggttaaaa attacggacg ggactgtgca atacgcgcct gcagccaggc ttccacttca
     2641 cttttcagcc agcgggagct gcgccccatt ttgatgggcg caggaaagcc cccatctttg
     2701 atgagtttgt aaaaccactt atctgtcagg ccggtcagtt gagtgataaa tgccatgtcg
     2761 accatctggt catccatcag tgaaactggg gtgatcatga gaatgttcct ccggatgttg
     2821 ttaaaccagc cggtgtggtc atgaaaacaa cgcaccacct tcaccggcat aacaaccggg
     2881 gagaaacgta aaaaataatc gggaatgtga ccgccaggag ataaatgggg aggttgcggt
     2941 ggctgagccg taccggtatg ccgggtggtt gttacgaaac gatgcttccg cacgcgcgcg
     3001 cgataaagaa gggatgattt tcgtgaggaa gaggggaaaa ctagcgttct gataattgca
     3061 gagttgttat cggaggtggt tgtggtgaat aagcctgtgg ctggcgggaa ggcaacagat
     3121 attaccgggg ggataaggct gtctgattgg tattataaat ataagaagtt atcagagagt
     3181 aacgtaaagg atttgccaga ataacctaag agagtaaatc tctccggtaa cagagcgctg
     3241 tgaagcattc agtatcagag tgcagataca tgaacgctga tgcgtaaaat cagcagtgac
     3301 acgccagctc cccctatgcc cattcctgta taagacatga actgagcgta ttaatgcttc
     3361 tgccctgaga ctgagccggt gagtgagggg ctgagaaatc cctcttgaca cagctctaac
     3421 cagaaatact tccccggttg ttgcggtgcg tccggatccc cgataacgga acactaaggc
     3481 tgtattgcgg gacaccgggt aagtggcagc ccgtaaggcg ttctgtcaac gccagaaaaa
     3541 atgatttccc acatgcgtta tagactcact cctgtggacc tggaagcccg taactctgcg
     3601 gttgcccacc gactgacgga actgaccgca aattttacga gtgcgtgctg gcttccgtct
     3661 ggaaatatgt cacctgaata ttgaagcagc gctacgataa gtccggtgtt atctgcaccg
     3721 gaggaccggg aacattaagc ggtatagcga caacaaagaa ttcgaaatca aaagggaatg
     3781 gtgccggagg cgtgggcggt aacaggctga ttttcaggta ttaccggctt acagcgtgtg
     3841 gttgatattg aggggctgtg agtttcgcgt atttgtcaga aacccagacg agttttcctg
     3901 tatgggacga aataaggcag cggcaataca tggaaaaatt taatatggta tgaaggagaa
     3961 cctgtcggtt acgcttaata taaccggtac actgtcagct caggggatac atgaaccgtt
     4021 atctcaccag ctacacagta tatacacact gacgctgaca gtagtcagca ggaccacaat
     4081 aatcagtgcc agcagtatgg taacgatacg gtctgtattc cggtaatagc cgaaggggtc
     4141 gacagaacca caatacgggc aacacacagt attatgagta atcgcgtttc cacagtccct
     4201 gcacttcttt ttgcgtatca ccataactcc ctccttacag ccttacccgt aaccggtttt
     4261 ttacattaaa aaaacaacgc tgaaaattca accgtcagtc cggagacgac cgttcgggtt
     4321 atcacagagt gcctgagaca gtgtcctgcc ggaggtcact tcagggactc tgcacatttt
     4381 ttacgacatg gctattccgt atgaaccata cggagattta accatgacct acaaatacaa
     4441 tcccttctgg cagcaacgta ttcgtgagac ggtgctgcac gcactgaatg ttcatccacg
     4501 cctgacggtg cttcgggttg acctgcgtct cccggatgtg ccggcagcaa cggacgcggc
     4561 tgtgatatcc cgcttcatca atgccctgaa agcccgaatc aacgcttacc agaagcgtaa
     4621 gcgtcgtgaa ggtaaacgcg tgcatcccac aaccctgcat tacgcctggg cccgggagtt
     4681 cggggtattc aggggcaaaa aacactatca cctcctgctg ctggtcaacc gggatacctg
     4741 gtgtcgtgcc ggcgattacc gcgatccggg gtcactggcc ggaatgattg aacaggcctg
     4801 gtgcagtgcc ctgggggtgg atgccggatg ctatgccacg ctggtgcatt ttccggtcag
     4861 gccggcggtg tggctggaac gcgatgatga tgctggttta cgggatatgc tgaagcgtgc
     4921 tgactatctc gcgaaagagt acaccaaagt caacggtacc ggtgagcgca attttggctg
     4981 tagccggagt tgaacacgac aggcgcacca atacccgaca aaccaaaagt atgggttgtt
     5041 gtctggcggc aacgatgtat ccactcgccc actgactgtt ttttacgtca gtgttccatt
     5101 gtgtgacaac cacagtgctg taacagcaac cagcctgctt tacccaaact ccagtccaga
     5161 ttttaactga accgccattc acaccctgac cacgctgccc gtaccgggac ggctgttgcc
     5221 tgcgtgtcgt ctggcagctg aggaatatga ctatgtacgc aaaatccttt atcgctcttg
     5281 atggcaacgg acgtctgacg ggcgcccgta ctgcacaggc cgcaccttat gctaactaca
     5341 cctgccactt gtgtggcagt gcactcagat accatccgca atacgacact gaacttccct
     5401 ggtttgaaca cactgacgac aggctgacag agcacggtca acagtgccct tatgtcaggc
     5461 cggagcgcag agaaatacag ttgattaaac gtctgcagca attcgtaccg gatgccttac
     5521 ccgtggtgcg taaagccagc tggcactgca gacaatgtca ccacgattat tatggggagc
     5581 ggtactgcac acactgccat acagggcact tcagtgagga gggagtggct gaatgaacgg
     5641 tataaaaacg gggctgggga tcacgccggg tgaacacatt atcagtgccg actctgcgct
     5701 cagcagaaat atccggcagt gtttctgcct ttcctgtcgt ggccggttga ttttgcagac
     5761 agatgctcag ggggcgtggt ttgagcatga tttacatgcc ctgagcgcgc aacagaaggc
     5821 ggcctgcgtg gtgctgaatc cggaaaagtc ccacccttac attgaggata tggcgatgtt
     5881 tctatcgcca ttaccggtcg tgctggagtg gcattgtgtg atgtgcggac agcattttcc
     5941 cgggaaaaag ttctgcgaag cctgtggcac ggggatctat tccagagcaa catgtacgaa
     6001 gtctgtttac agctaccagc cagatttgta ccaggactgc ggaggtgcga gtacttaatc
     6061 caaaatatcc gctctgcgca gggcgttcag aggtattgcc agcctgcatt ttatccggtg
     6121 tttcgcacct accccgttcg tgacacaaag gcgcgacacc ggatatccgc agtcctgctt
     6181 acctgaataa aacccggggt catgattgtg tgctcgtgga acgaaatcgc accgtgatcg
     6241 cggattcgcc gaatctgcac tgtggtgtca ataaaccaca cgcgagagtc ctctccgtat
     6301 tgatcagtgc atcaggacga tttctgcatt gttaatccag ggggctgcat tattcatttc
     6361 cctgctaatg gacgttacat cctggatatt catgatcctg atttctctga gcagtatcag
     6421 cgcttattgg agcgtctgga ttatctgaag aagcccggta ctaaagcatc aggccagcgt
     6481 aactctggct acagcggttt ttgacctgtt gtaactgtaa ggtgatttat tatgtataca
     6541 gaatcaaacg ttaatctttc ctgtaacgcc ctctggcaaa agtgtgtcct tgcaactctg
     6601 tatcgggctg cagccacaca tcctgaaatt aaattattac aatttaaatt aataccaggg
     6661 cagggcctta ataaggccat tccctctctg atggctgatt ttactggtga actgcataga
     6721 ctcatttctg aatatttgca gtgtaagcga cgtaaagata aatctgttga gcccacccct
     6781 ttatattatc tctggagcag ggaggctgtg gctaacgata ccactcctta tcttatggtg
     6841 ctgctactta ataacgatgt ctggtgtaag agtcgtgagg tggtggttct cagaaatctg
     6901 attgagcagg tgtggggaaa agtatggggt gaagataact ccattacaca tctggttgtg
     6961 tatcctgaag tgctgtcgtt ggtatttgat gcagctaaca catacaacgc tgagcctggt
     7021 gtactggaac agacagcccg ggcttacatt gaatcgcatg gtgctgttga acgtcatctt
     7081 gggtgtagtg acttttaacg tcaggtgacg cattgtcact tctgacctgc tacctgtacg
     7141 ccggaatata atggccagag aggcatattt cgacgcccct gatctggggc gtttgattca
     7201 ggatgtctgg aacaatccgg gctttgtgtc accgggcgca tcaaagcagt accacccggg
     7261 atgtggcgct ggatcgtcat ttaccggact gcgaagacat acttaaagag gtgatatggg
     7321 cgttcagtga ttttgtccgc gaaatcgtgg tgtgtatgat ccggaagccc gttaccccgc
     7381 tggtaatccc tggtatcccg taacaggcca gttctgaaag cacagctcag cagattcccc
     7441 gcattcgcac aatcttcata aatctcacag aggacattct gaccatgttg actacaacaa
     7501 gccacgacag cgtatttctg cgtgccgata attccctgat tgacatgaac tatatcacca
     7561 gtttcaccgg catgaccgat aaatggtttt acaggctgat cagtgaaggg catttcccta
     7621 aacccatcaa actgggtcgc agcagccgct ggtacaaaag cgaagtggag cagtggatgc
     7681 agcagcgaat tgaggaatca cgagaggcag cagcatgaaa tgtgttgtga tgccagtacg
     7741 ctggcaatgt gcaaaatgtc ggcgctggta ttgtggaaat cagccctgtc cctggtgctg
     7801 gcgatattac cgttcatctt cctgctgata ccctctggtc agccagcggg gtgttatccc
     7861 tttctgacgt attcgtcatt ccattcgtat caccattcac tggtgtttgt atcgtatcgt
     7921 gccgatgttg atgcgtattt tcctgtgtgt ctgcaccggc ttaacaaaat tcaacagagt
     7981 ctgaaggagt cgtattccgt gcaaataacc gaagcgttac ttttagtgac ggggttatcc
     8041 aatacttttc tagtcagtgt ttagtattta tgcaagcgat gtaagtagta accgcttaga
     8101 ctattgacat tcatgatgcc tgttaattca aatttcaggt atgatctgtc atttattggt
     8161 gtgatactga ttccggtagg ttggcataac tgtcctgcaa atcattgttc aaaaaagaaa
     8221 gctcctacaa actgtatcct gtatctctac cagaagataa gatacttggt tatttttgtg
     8281 cataagctca cataaaaata atattaatta tgttttctta gcgatagaaa aataaacagg
     8341 taagcagaag acggactttt ttaatgcgaa aacgtattct ttttattggc ccaccgctgt
     8401 acggtttgtt atacccattg atttctctgg ctcaggcctt tcgtgtaatc ggacatgatg
     8461 tagtaattag tagtgctgga aaattcgcga ataaagcagc agaagctgga ctggttgttt
     8521 ttgatgcagt tccaggttta gattcagagg ctggatatcg ccatcaggaa gagttgagga
     8581 aaaaaagtaa tattattggt catttctctt tttttagcga tgaaatggca gataacctca
     8641 tcgattttgc aggaaaatgg aggccagatt taatagtcta tcccccgctt ggtccggcag
     8701 gcccattggt tgctgctaaa tatagaattc cttcagtgat gctggctgtt ggattcgcgc
     8761 atacatctgc ccatattcag atgttaaatc gttctttaag taatgcttac aggcggcatg
     8821 gagtcagcgg tccactatgt gatttagcat ggattgatgt tgctccccca agtatgagca
     8881 ttcttaaaaa tgctgaagaa ccggttatct caatgagata tattccttat aacggaggtg
     8941 ctgtaaagga aacatggtgg gacagggatt ctgatcgaaa acgtttactc atcagccttg
     9001 gcactgtaaa accaatggtt gatggtctgg agctgatttc atgggttatg gattctgcaa
     9061 atgaagttga tgctgatatc attttgcaac ttgcaataaa tgctcgtact ggattacgaa
     9121 aactaccatc aaatgtacgt ctggttgact ggatacctat gggtgtattc cttaatggag
     9181 ctgatggatt tattcatcat ggtggcgcag gtaataccct gacagcgttg tatagtggga
     9241 taccacagat tgtgtttggc gaaggtgcag atcgctctgt taatgcagaa attgttgcga
     9301 agcgtgggtg tgggattatt ccggacaagc atggactgac cagtgatttg gtaaatcgcc
     9361 tgctttatga tgattcacta cgcttctgtt cagatcaggt agccgctgaa atggctgaac
     9421 aacccagtcc tgcagagatc gcagaggttt tgatgagaaa attaaaaaac aacgggaaat
     9481 aattgtatag tattgctgat attatcgacg aaaaatgtca tgagtaatag aatgtccttc
     9541 acttatatga tgaaggacat aactaaataa atgagtatgt ctcccgttat aatgttgcag
     9601 aaagcgttat cggtacgatg agataatcgc ttacacaaag ttattatctg atatcttcct
     9661 gcaattataa tttgctaatg catcaataat tgctccgcgc caccaggcat aatcatgacc
     9721 accggcatag acagtgaggt tactttccaa acctgctgcg attaacgact gatgcaaccg
     9781 ctgaacatgt gagacggttg taccttctaa agaaccgact ccaagttgga tatttacatc
     9841 tttcggaggc gctgaaagta tctgctcact tacccaggag gtatcattct cagtaaacaa
     9901 aatcgggctg ccctggtcag ggttccacca cattgaaggt gagtggctaa tgattgtacc
     9961 aaatgttgtc gacgcatata tagctgccat cagtgctgtc actccaccga ggctctgacc
    10021 ggccagtata gtattagaac gaccagccca tacgatatcc gggtagtctc tgtataactg
    10081 gggtatcaga ttttccgcta tatcaaagat aagttcttta ttgcctccca gtatattcat
    10141 acgatcagat tcattaatat tgtctatccc cataattgcc attggcgtaa tatgaccagt
    10201 ggtaatggcc atatctaatg cccgggtaat atccatccgg tcaaaccata tttcagcatc
    10261 aggtaacaca agtaatccaa gaggtactga tgttggattc tggggaaaat ataaacgaat
    10321 ccggcgttga tatcctgcaa caaagtaatg taatgttgaa agaatacccg tacatttatg
    10381 ggaagtatca tcccattcct tttgttcagg agccatatca agagaaagga ctgattctcc
    10441 gaatcctcgt atatttattt ctgctgtttt gttaaatgga tcagatttac cgggtaatgg
    10501 agagaagcga cttcctagtt gaaatatgtc tttttgtgtc atatctgtgg gaatttctat
    10561 aaatgagtat gagccccgat atgaagctgg taacaccagt gtcagcatcc agatatctgt
    10621 cgaagggata tgagtcatta gtcctttttt gacatctttt ttatctgtaa cacgattcag
    10681 gcgaagatat acgccttgca ctgctttctc tgagcgccac agaaatgtaa cctcccgatt
    10741 ttctgtagtg gtgctttttg tgatcactgg aagagttgct gactcaagat agtgccagaa
    10801 attagcaata tctgttgttc ttattgtgac aggatgtgtc atttttttta actgtacaca
    10861 aatatcttcg ggagtctgga aaagatagtt tttcataggt atcatcttaa attaatccaa
    10921 gtgtaagaat aatacatcaa atgacgttca ttcaaggggt gatgagtgac tttccggaat
    10981 taaatc
//
LOCUS       DAZFFG010000134        10968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFFG010000134 DAZFFG010000000
VERSION     DAZFFG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.134"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            92..853
                     /gene="adiY"
                     /locus_tag="ACHJ7J_004177"
     CDS             92..853
                     /gene="adiY"
                     /locus_tag="ACHJ7J_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO5002341.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            990..2327
                     /gene="adiC"
                     /locus_tag="ACHJ7J_004178"
     CDS             990..2327
                     /gene="adiC"
                     /locus_tag="ACHJ7J_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO5002342.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2431..4074
                     /gene="eptA"
                     /locus_tag="ACHJ7J_004179"
     CDS             2431..4074
                     /gene="eptA"
                     /locus_tag="ACHJ7J_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO5002343.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4071..4739
                     /gene="basR"
                     /locus_tag="ACHJ7J_004180"
     CDS             4071..4739
                     /gene="basR"
                     /locus_tag="ACHJ7J_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO5002344.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4740..5840
                     /gene="pmrB"
                     /locus_tag="ACHJ7J_004181"
     CDS             4740..5840
                     /gene="pmrB"
                     /locus_tag="ACHJ7J_004181"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO5002345.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESTADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5816..5905)
                     /gene="pmrR"
                     /locus_tag="ACHJ7J_004182"
     CDS             complement(5816..5905)
                     /gene="pmrR"
                     /locus_tag="ACHJ7J_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO5002346.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6017..7519)
                     /gene="proP"
                     /locus_tag="ACHJ7J_004183"
     CDS             complement(6017..7519)
                     /gene="proP"
                     /locus_tag="ACHJ7J_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO5002347.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7783..8661)
                     /gene="rdcB"
                     /locus_tag="ACHJ7J_004184"
                     /pseudo
     CDS             complement(7783..8661)
                     /gene="rdcB"
                     /locus_tag="ACHJ7J_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            complement(8658..10886)
                     /gene="crfC"
                     /locus_tag="ACHJ7J_004185"
     CDS             complement(8658..10886)
                     /gene="crfC"
                     /locus_tag="ACHJ7J_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO5002348.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIDQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGWGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYIDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2746 a         2869 c         2691 g         2662 t
ORIGIN      
        1 tttatattca tcatacaatt atataaccat ttcccggtat cgctttgctt tagcgagaac
       61 tggtctttta ttcgcactca ggagtacatg tatgaggatt tgcagcgacc aaccttgtat
      121 tgttttattg actgaaaaag atgtctggat aagggtgaat gggaaagaac ctattagcct
      181 taaagctaac catatggcgt tattaaattg tgaaaataat attatcgacg tctcctctct
      241 taacaacact ttggttgctc atattagtca cgacatcatc aaagattacc tccggtttct
      301 gaataaagat ctctcgcaaa taccagtatg gcaacgtagc gctacgccca ttctcaccct
      361 gccatgcctg acaccagacg tctttcgcgt tgccgcgcaa cacagcatga tgcccgcaga
      421 aactgagtca gaaaaggaac gaacacgcgc attattattc acggtgctat cccgttttct
      481 cgacagtaaa aaattccttt cattaatgat gtatatgtta cgtaattgtg taagtgacag
      541 cgtttatcaa attattgaaa gcgatattca caaagactgg aatcttagta tggtagccag
      601 ttgtttatgt cttagcccaa gtctgttaaa gaaaaagctg aaaagcgaaa acaccagtta
      661 tagccaaata atcaccacct gccgcatgcg ttatgccgta aatgaattaa tgatggacgg
      721 taaaaatatc tctcaggtat cacagtcctg cggctacaac agtacgtcgt actttatttc
      781 tgtctttaaa gacttctacg gtatgacgcc gctgcattat gttagtcagc acagagaacg
      841 cactgtcgcc tgatttttaa ccttaacgaa gagctatatt aataacggca tcagcgataa
      901 cccggtcgat aataattcaa ctatcgaatg caggcgtatg atatgacgta atttattgtc
      961 acgaagctcg ccttcgcagg agtttaatta tgtcttcgga tgctgatgct cacaaagtgg
     1021 gcttaatccc cgtcaccctg atggtgtcgg ggaatattat ggggtcaggt gtttttctgt
     1081 tacctgcaaa cctggcctcc actggcggga ttgccattta tggatggttg gtgacgatta
     1141 tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc gttcctcgac ccaagtcctg
     1201 gtggttctta cgcttacgcc cgccgctgct ttggcccgtt tctcggttat caaaccaacg
     1261 tcctctactg gctggcctgc tggatcggca atatcgccat ggtggtcatt ggcgtaggat
     1321 atttaagtta cttcttcccg attctgaaag atccattggt attaaccatc acctgcgtcg
     1381 tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc gaaaatgatc acccgtgtgc
     1441 aggccgttgc caccgtgctg gcgctgatcc ccatcgtcgg gattgccgta tttggctggt
     1501 tctggttccg tggtgaaacc tatatggcgg catggaacgt cagcggcctg ggcactttcg
     1561 gtgcaattca aagtaccctt aacgttacgc tgtggtcgtt catcggtgtg gaaagtgcct
     1621 ccgttgccgc aggtgtggtg aaaaacccga aacgcaatgt ccctatcgcc accatcggtg
     1681 gggtattgat tgccgccgtt tgctatgtgc tctccaccac cgcgattatg gggatgatcc
     1741 ctaatgccgc actgcgcgtt tctgcctcgc cattcggtga tgccgcacgg atggcgttgg
     1801 gtgacaccgc cggggccatt gtttccttct gcgcagctgc gggttgctta ggttcactgg
     1861 gcggctggac attgctggcg ggtcaaacgg cgaaagccgc tgccgatgac ggactgttcc
     1921 caccgatttt tgcccgtgta aataaagcgg gtacgccagt ggcggggctg attatcgtcg
     1981 gtattttgat gaccatcttc cagctcagca gcatttcacc aaacgcgacc aaagaattcg
     2041 gtctggtgtc ttccgtctcg gtcatcttta cactggtgcc atatctttac acctgtgcgg
     2101 cgttactgct gctcggacac ggtcactttg gtaaagcacg cccggcatat ctggcagtta
     2161 ctaccattgc cttcctctac tgcatctggg ccgtggtggg gtccggggcg aaagaggtta
     2221 tgtggtcatt tgtcaccctg atggtcatca ccgccatgta tgcgctgaat tacaaccggc
     2281 tgcataaaaa cccgtatccc ttagatgcac caataagcaa agattaattc cccttaatcc
     2341 agcaaagata aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat
     2401 atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagaccctct
     2461 ttgaatttac tcgcctggct attgttggcc gctttttata tctctatctg cctgaatatt
     2521 gcctttttta aacaggtgtt gcaggcgctg ccgctggact cgctgcataa cgtactggtt
     2581 ttcttgtcga tgccggtcgt cgccttcagc gtgattaata ttgtcctgac actaagctct
     2641 ttcttatggc ttaatcgacc gctggcctgc ctgtttattc tggttggcgc ggctgcacaa
     2701 tatttcataa tgacttacgg catcgtcatc gaccgctcaa tgattgccaa tattattgat
     2761 accactccgg cagaaagcta tgcgctgatg acaccgcaaa tgttattaac gctgggattc
     2821 agcggcgtgc ttgctgcgct gattgcctgc tggattaaaa tcaaacctgc cacctcgcgt
     2881 ctgcgcagtg ttcttttccg tggagccaat attctggttt ctgtactgct gattttgctg
     2941 gtcgccgcac tattttataa agactacgcc tcgttgttcc gcaacaacaa agagctggtg
     3001 aagtccttaa gcccctctaa cagcattgtt gccagctggt catggtactc ccatcagcga
     3061 ctggcaaatc tgccgctggt gcgaattggt gaagacgcgc accgcaaccc attaatgcag
     3121 aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg ggcggagaac
     3181 ttctccctca acggctaccc gcgtgaaact aacccgcggc tggcgaaaga taacgtagtc
     3241 tatttcccta ataccgcatc ttgcggcacg gcaacggcag tttcagtacc gtgcatgttc
     3301 tcggatatgc cgcgagagca ttacaaagaa gagctggcac agcaccagga aggcgtgctg
     3361 gatatcattc agcgagcggg catcaacgtg ctgtggaatg acaacgatgg tggctgtaaa
     3421 ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc tggtcagtgc
     3481 atcaacggcg aatgctatga cgaagtgctg ttccacgggc tggaagagta catcaataac
     3541 ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggtcc gacctattac
     3601 aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacaccaa tgagatccag
     3661 acctgtacca aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat
     3721 attgttgata aagcgattaa tctgctgaaa gaacatcagg ataaatttac caccagcctg
     3781 gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg
     3841 ccttatgcca tcgccccgga tagccaaaaa caggtgccga tgctgctgtg gctgtcggag
     3901 gattatcaaa aacggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa
     3961 cactattcac aagacaattt attctccacg ctattgggat taactggcgt tgagacgaag
     4021 tattaccagg ctgcggatga tattctgcaa acttgcagga gagtgagtga atgaaaattc
     4081 tgattgttga agacgatacg ctgttattgc agggactgat tctggcggcg caaaccgaag
     4141 gctacgcgtg tgatggcgtg acaaccgcgc ggatggcgga acaaagcctt gaggcaggtc
     4201 attacagcct ggtggtactg gatttagggt tacccgacga agatggactg cattttctcg
     4261 cccgtatccg acagaaaaaa tataccctgc cggtactgat cctcaccgct cgcgatacgt
     4321 tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga ctatctggtg aagccttttg
     4381 cgctggaaga gttacacgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg
     4441 aaagtgagct gattgttggc aatctgacgc tgaatatggg tcgccgtcag gtatggatgg
     4501 gcggtgaaga gttgattctg acgcccaaag aatatgctct gctgtcacgg ttaatgctca
     4561 aagcaggcag tccggtgcat cgggaaattc tctacaacga catctataac tgggacaatg
     4621 aaccctcgac caacaccctg gaagtgcata tccacaatct gcgcgacaaa gtgggcaaag
     4681 cccgtatccg caccgtgcgc ggctttggct atatgctggt cgcgaatgag gaaaactaat
     4741 tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg ccaacggctg atattgacca
     4801 tcggggccat tttgttggtg tttgagctga tcagcgtctt ctggctatgg catgaaagta
     4861 ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca
     4921 tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtca
     4981 gcctgacgct atttatctgc tatcaggcgg tacgccgcat cacccgcccg ctggcggagc
     5041 tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac gcccattgcc attcacagcg
     5101 ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga tctggtcagt cgcctgacca
     5161 gcacgctgga taacgaaagg ttatttaccg ctgacgtcgc gcacgaactg cgaacgccac
     5221 tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac
     5281 cgttagtggc acggcttgat cagatgatgg agagcgtctc ccagctgctg caactggcgc
     5341 gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca
     5401 ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa accctgctat
     5461 tgccagagag caccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat
     5521 tgcgaaacct ggtagaaaac gcccatcgtt acagcccgca aggcagcaac attatgatta
     5581 agctgcaaga agatggcgga gcggtcatgg cagttgaaga tgaaggacca ggtattgatg
     5641 agagtaaatg cggggagttg agtaaagcgt ttgtacgaat ggacagccgt tatggcggga
     5701 ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc
     5761 tgcaaaaccg gcaagagact tccggcacgc gggcctgggt acggctgaag aaagatcagt
     5821 acgtggcaaa ccagatataa agaaagctgc tgaccaccag cacgctgaac acggtagtta
     5881 aactttcata aacacggttt ttcattgcac tctcctccgt taacctggag gagagtatgc
     5941 gcgtcagaga ttaagtgaac cttaagagtt caattggcct gataagacag cgttacatca
     6001 ggccatccgt ttcagcttat tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt
     6061 tcgcctgcaa atcggcaatc tcgtggtcaa tatcatcgat tttctgctcg atattatcgt
     6121 aatgctcgac gagaatttcc ttcgcttcct gtatatctga cgccgccggt gttgcacctt
     6181 tcaacggacg atttgccgtc tctttcatgg ttacgccggt gattaaacca accaccgcca
     6241 ctaccatcag gtaataggct ggcatcatca gattttgcga gctttcgacc agccaggccg
     6301 ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc
     6361 tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga agccataacg cccgtaaagc
     6421 agttaaggat caccgccagc atcagtaacc cggcaaaaat caggccgatg acgttactgt
     6481 taatcagaat aaacgccggg atcgccaaca caaacagtgc aacactacca agtagcacaa
     6541 acggacgacg gccaaaacgg tcactcagca agcccatcac cggctggaca aacagcatac
     6601 cgatcataat ggcgataata atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg
     6661 acaaataact cggcatatag gtcagcaaca tgtagtaagt cacgttggtg gcaattacca
     6721 gaccaataca tgtcaacagg ctgcgccagt atttagtggc aatctcttta aacgagactt
     6781 tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag tttatcaaca tgctgctgga
     6841 acgccggagt ttcttccagc gcatggcgca ggtaaagccc gataatccct aacggcagag
     6901 caataaagaa cggaatacgc cagccccagt cgaggaagtt cgcttcgccg acaatggtcg
     6961 aaattaacac caccacgccc gcacccagca caaacccggc aatagaaccg aagtccagcc
     7021 agctgcccat aaagccacgt ttacggtcag gggagtattc cgcaacaaat atcgacgccc
     7081 cggtatattc accgccgacc gagaaacctt gtgccatctt acagatcaac agcagaatcg
     7141 gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc aatacagaac gtactgatcg
     7201 acataatcac aatagtgata gcgaggatct tctggcgacc atatttatcg cccaacatac
     7261 caaagaagag tccgccaagc ggtcgaatca gaaagggaac ggagaaagtg gcaagtgcag
     7321 caaccatctg cacgctgggg tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa
     7381 caaaaccata aacaccaaaa tcaaaccatt ccattgcatt acccagtgat gctgcggtaa
     7441 tggctttacg cagtttaccg tcatcaataa tggtgacatc acgaagggta atcggtttta
     7501 cttttttcct tttcagcata gctttcctcg cagagttggc ccatttccgc aaacgccaga
     7561 aaaatcagcg cgacggccgc aaaaggggag cgggaattgc aggatgcaat ctctgtaaca
     7621 agcgtagcgg gtttgcttac accctccggt taaggaatgt acaatcaaac cagttgacgc
     7681 ctgaattggg cagttaatga cctttttacc ctaccagtgt ttaaatttgt gatcaacttc
     7741 acacatggat ttcgtttagg actcattgat gtaactgatt atttacacca aatttacaga
     7801 gaagatttcc tcgagcattg cccccgccag acgctgcgct gaacagagtc gtggcatccc
     7861 cagcgcgaca gtttgccagc gttaggtcac tgaccagcaa acgggttgtt tatctgccgt
     7921 ggtccacttt cccagccaac ttaaaaaatc agcatgatcc ccgcccgtcg gaacggtcga
     7981 agaggcaaac cactgatggt aatagtgacg cgtggtcggg gccgcattga tactctctgc
     8041 cagatgttgt gtcgctttca cgcgcaaatc acccagcaac aacgccaccg cttgcgggct
     8101 tcccagacga caggcatcgc caaatgcccc ccaacgcaac tcttcgagaa cgataaaaca
     8161 gcgcccaggc aacgaccagg cgttatacgc cccagcacgc cagcgggtaa aaatttgctc
     8221 gctctgttca cccgcctgca ccagtaggcc tcgttgcgtt aacgcctttt ccttccagcg
     8281 acaacgggct gaaaaccact cacacaaggc ggcaatttgc tgggggagct ccgggaaagc
     8341 agagtgctgg cggtgatcca ttttttgcag cagcgccaga tgctgcgcga tctgcgtcag
     8401 tgccagatga ccgggatcca gcatcgacgc cagtttttcc accaatgtca gacgcaggct
     8461 aagcggcgca tttaacatcc cgccgttcgt ccatgtctga agctgcacgc gcgcaagaag
     8521 ttcctgcatc agtcgttcac gaaactgctg ctgatgggct tgtggaaggc gggcatcatc
     8581 gccctgcgcc agatccacta aaaagcgcgg atgaaccgac tccagcacgc gaccggggcc
     8641 gtccagtaac gtcttggtca atatcgttct gccgtgaaaa gtgtttgaat atcatcgcgt
     8701 aacagctgag cgtcgatgta aatccagccg tgagtcatca cagtctgctg caattgctgt
     8761 tgcatcagcc tgaccaccga ttcattttgt tgacgcagag ccagactttc gcgtaaacgc
     8821 gtctgtaatt ccgtcaaaca tgaagcgaac tcagcgaaaa aagtattcat gcctgccgta
     8881 acagattcat cgacctgctc tgccagaact ttagccattt gttggcaata aagatcgact
     8941 tctgcgctta atgctcgttg caacacactg taatcaaccg tctctgtcgg ggatttctca
     9001 tttccccatc cccagtcggg ctgattcaac cagcgcgaaa aagtctcacg cacaacgcct
     9061 aaacgcgtgc tctgctcgtc cgttgcttcc tgccgcgaaa tgacttcact gaacagctgg
     9121 cgagtattga agtggggaac tgcgccgtga aaaacaggaa aatgaaaccc aggacgaaac
     9181 cctgactcgc tcaattccat tttgacttgt tggtcaatgg ggcgaataac atcggttaac
     9241 acccggcaaa gggtggattc aagctcggca aaacgcagcg taaagtcgcg actgatggtg
     9301 ttctgcgccg tctgtaacag tgtctcacag cgggtacgaa tctcgcttaa cggctccgaa
     9361 tcatcctgaa acaaggcggc taactgcgcc ttcagcgcat cttgctgttg acgcagaaag
     9421 tggttggcgg aggtcagggc cagctcgatt tcatgtttaa tctcgccgct cacctgcgcc
     9481 tgattgagtt gcaatagctg caaactttct tcgatctggt ggatattttg ccgcaattgt
     9541 tcacaagcga cgtttaaccc gtgcgcacga aaatccaggt attcccgcgc ctgctgcgca
     9601 taattcaaca gtttatgcgc ggcagatcgc agagcataca acgaggcgtt agcgtaagcg
     9661 gcatgaagca acgcctgaat tggctgggcg aacagcgaat cttcccacaa ctgatcggca
     9721 gcatgacgaa tatgttcgag gtccgccaga tcggcatgac gccagcgcct gccgagcgcg
     9781 gcatgggcaa aatcttccac ccagcgttgt tgctctggcg ctggtaactt accgttgttg
     9841 gctaactcat ggcgcgcccg atttgccagg tagccccaca tcgacgacac cggaaatatc
     9901 tgctgtggcg taatacagcc tttcatcagc gtcccggaaa tcagtgcccg cacctggtcg
     9961 gcgtcgtcac tgttacgatc ctgttgatcg aacttattga ccagcacata cagcggcacc
    10021 gattgcccca ccgccaaaat cgcctcacgg acctcttcat cggagatcga tttcagttgc
    10081 gtataatcca gcaccgccag taccgccgag gcgcgagcca gctgctggtt aagcattttt
    10141 tgcagatgcg gttgcccggc ttcatttggc ccgggggtat ccagtaacgt caactgaccg
    10201 ggataactct ccagccccgc cagatggaca aactccactt caatcacggg aatatgctca
    10261 atggcggcgt aagcagaaaa aggaaaatcg acgtccagcg ccttcgccag tcgcaccaaa
    10321 tcattcaaac ttttcagaca atgaaaaata ggctgggcac ccagataata tttttcgaaa
    10381 gcgacgccat tttcgatccg ctgcataagc gcacgcatat ctttatctat ttccagcaca
    10441 tcggtcagat gcttaatatc gcaatcacgc aggcgctgtt gtaatttttg aattaaacaa
    10501 tcgattggcg cgacatgtga aaaatgcagt accggttctt tttgcccggg tgtatggcga
    10561 ataagcgtcg gcagcgcagt cattgggcga ttgcgattag gcagtacctc cgtaccaaca
    10621 atggcattaa tggtggttga tttccctgct ttcatggtac cgacaatcgc cagcaccatt
    10681 tccagtcggg aaattttacg caactcatta ttcagcatcg cgtgacgctc ggcgatatta
    10741 ggctgactcc agggtaaagc cagttgtggc gcatcgtctc cgggtacaga gagaggcatt
    10801 ttttccagta actgcaactg ttggcgagaa agctgtaaca gccgttcagc ctcctgactt
    10861 aactcataca gggtctgtgt gtacatagaa aattcttcct taaagcaaat ttaattactt
    10921 ttatttagcc agattgtttt tgagttctgt tttcggcttt tataatta
//
LOCUS       DAZFFG010000135        10942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFFG010000135 DAZFFG010000000
VERSION     DAZFFG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.135"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            51..1193
                     /gene="yghO"
                     /locus_tag="ACHJ7J_004186"
     CDS             51..1193
                     /gene="yghO"
                     /locus_tag="ACHJ7J_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO5002349.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1200..1964)
                     /gene="glcC"
                     /locus_tag="ACHJ7J_004187"
     CDS             complement(1200..1964)
                     /gene="glcC"
                     /locus_tag="ACHJ7J_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO5002350.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2215..3714
                     /gene="glcD"
                     /locus_tag="ACHJ7J_004188"
     CDS             2215..3714
                     /gene="glcD"
                     /locus_tag="ACHJ7J_004188"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO5002351.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILVVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3714..4766
                     /gene="glcE"
                     /locus_tag="ACHJ7J_004189"
     CDS             3714..4766
                     /gene="glcE"
                     /locus_tag="ACHJ7J_004189"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO5002352.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4777..6000
                     /gene="glcF"
                     /locus_tag="ACHJ7J_004190"
     CDS             4777..6000
                     /gene="glcF"
                     /locus_tag="ACHJ7J_004190"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO5002353.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISIMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6005..6409
                     /locus_tag="ACHJ7J_004191"
     CDS             6005..6409
                     /locus_tag="ACHJ7J_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO5002354.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6431..8602
                     /gene="glcB"
                     /locus_tag="ACHJ7J_004192"
     CDS             6431..8602
                     /gene="glcB"
                     /locus_tag="ACHJ7J_004192"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO5002355.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNDKETTLRTPAQFVG
                     YRGNAAAPSCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8957..10639
                     /gene="glcA"
                     /locus_tag="ACHJ7J_004193"
     CDS             8957..10639
                     /gene="glcA"
                     /locus_tag="ACHJ7J_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO5002356.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2556 a         2740 c         3064 g         2582 t
ORIGIN      
        1 tgctgttgca acctgttttt caccgttaat tatttgttac ccacagaact atggaatgcg
       61 atctgctgat gatcaaaatt gaaaaagtaa ttaataaaaa tgaccttaag gcatttatcg
      121 cttttccgtc atcactttat cccgacgatc caaactggat acccccttta ttcattgaac
      181 gcagcgagca tttgtctgcg aagaatccgg ggacggacca tatcatctgg caggcgtggg
      241 tggcaaaaaa agcggggcag atagttgggc gtattaccgc gcaaatagat accttgcacc
      301 gtgagcgtta cggtgaagat accggtcatt tcggaatgat tgacgccatt gatgatccgc
      361 aggtttttgc cgcgttgttt ggcgcagcgg aagcgtggtt gaagtcacaa ggtgcaagta
      421 agatcagcgg gccttttagc ctgaatatca atcaggaaag cggattactg attgaaggtt
      481 ttgacacacc accctgtgcg atgatgccac acggcaaacc gtggtatgcc gcgcatattg
      541 aacaactggg ttatcacaaa ggcattgatt tactggcgtg gtggatgcag cgaaccgatc
      601 tcactttctc tccggcgcta aaaaaactga tggatcaggt gcgcaaaaag gtgaccattc
      661 gctgcatcaa tcgtcagcgg tttgccgaag agatgcagat cctgcgtgag attttcaact
      721 ctggctggca gcacaactgg ggatttgtgc cgtttaccga acatgaattc gcgaccatgg
      781 gcgaccaact taaatatctg gtgccggacg atatgatcta catcgctgag attgattctg
      841 caccctgcgc gtttattgtc ggcttgccga acatcaacga ggcgattgcc gatctgaacg
      901 gatcgctctt tcccttcggc tgggcaaaat tgctgtggcg tttgaaagtc agcggtgtgc
      961 gaactgcgcg agtaccgctg atgggcgtgc gcgacgagta tcagttcagc cgcatcggcc
     1021 cggtgattgc gctgttattg attgaagcct tacgcgatcc gtttgcccgc cggaagatcg
     1081 atgcgctgga gatgtcatgg atcctcgaaa ccaatacagg catgaataac atgctggaac
     1141 gcattggggc ggagccgtac aagcgttacc gattgtatga aaagcagatt taataccagc
     1201 taactcaggt tcatctccag cggcaccgcc gagcgaatca aatggtggcc ttccagctcg
     1261 atatcgtgga gattcttttt cacggtccgc acatgatcgc gtgctgcgcg ctgggcgacg
     1321 tgcggcagcc gctgcaacac cgcgttgtag atccgcgcat gctggcgatc gatctgcttt
     1381 ttttgctgtg gtcgatggta gagattattt acgctggcaa acactgaatt aaacatcagg
     1441 tcggtcaatg attgcagcgt aaacaccagc acctgattgt gagaagcctg acaaatggcg
     1501 agatggaaag cgtgatccaa ctgcgcatgt tcgatcagcg aaatctcttt gttgttctca
     1561 ctggcggcga gcattttttc ataacagcgg gttatcacaa caaaatcagc ctgcgttccc
     1621 agcgttgccg ccagccttgc cgattcgccc tccagcaatg cgcgaacgtc gagcagatcg
     1681 tacagcgttc gcggctgcgt actgaacaga tggatcagcg ggctggtgtc ctgcacccga
     1741 ttaagccgtg cgacacgaga atcgcgaccc tgcgccgttt caataatccc gcgcccgcgc
     1801 agcacggtca gcccttcacg cagtgcggag cgtgagaacc cgagcttttc acacagtcga
     1861 cgttccgagg gaagcggctg accgaccttc agtacgccgt cgataattaa ccgttcgata
     1921 ctctctgcaa ccacttcgca aatagggcga cgttcatctt tcattcccgg acctcgtgca
     1981 caggtaggac caatttttct gttcttaact cgcaaaacac gcacatcacg taagtgatta
     2041 tgttacatca atttaacatt gagttaacca agacaaggtc acagagctgg aaaaaaaatg
     2101 gtctgaccgg tagctaaaga gatagacgaa aacgaaaagc ccgcttaata actgttcaca
     2161 gaagcagcgc gcaaaaatca gctgccacac aacacaacaa agcgaagcct actcatgagc
     2221 atcttgtacg aagagcgtct tgatggcgct ttacccgatg tcgaccgcac atcggtactg
     2281 atggcactgc gtgagcatgt ccctggactt gagatcctgc ataccgatga ggagatcatt
     2341 ccttacgagt gtgacgggtt gagcgcgtat cgcacgcgtc cattactggt tgttctgcct
     2401 aagcaaatgg aacaggtgac agcgattctg gttgtctgcc atcgcctgcg tgtaccggtg
     2461 gtgacccgtg gtgcaggcac cgggctttct ggtggcgcgc tgccgctgga aaaaggtgtg
     2521 ttgttggtga tggcgcgctt taaagagatc ctcgacatta accccgttgg tcgccgcgcg
     2581 cgtgtgcagc cgggcgtgcg taacctggcg atctcccagg ccgttgcgcc gcataatctc
     2641 tattacgcgc cggatccttc atcacaaatc gcctgttcga ttggcggcaa tgtggctgaa
     2701 aatgccggcg gcgtccactg cctgaaatat ggtctgaccg tacataacct gctgaaaatt
     2761 gaagtgcaaa cgctggatgg cgaggcgctg acgctgggat cggatgcgct ggattcgcct
     2821 ggttttgacc tgctggcgtt gttcaccgga tcggaaggta tgctcggcgt gaccaccgaa
     2881 gtgacggtaa aactgctgcc gaagccgccc gtggcgcggg ttctgttagc aagctttgac
     2941 tcggtagaaa aagccggact tgcggttggt gacatcatcg ccaatggcat tatccccggc
     3001 gggctggaga tgatggataa cctgtcgatc cgcgcggcgg aagattttat tcatgccggt
     3061 tatcccgtcg acgccgaagc gattttgtta tgcgagctgg acggcgtgga gtctgacgta
     3121 caggaggact gcgagcgggt taacgacatc ttgttgaaag cgggcgcgac tgacgtccgt
     3181 ctggcacagg acgaagcaga gcgcgtacgt ttctgggccg gtcgcaaaaa tgcgttcccg
     3241 gcggtaggac gtatctcccc ggattactac tgcatggatg gcaccatccc gcgtcgcgcc
     3301 ctgcctggcg tactggaagg cattgcccgt ttatcgcagc aatatgattt acgtgttgcc
     3361 aacgtctttc atgccggaga tggcaacatg cacccgttaa tccttttcga tgccaacgaa
     3421 cccggtgaat ttgcccgcgc ggaagagttg ggcgggaaga tcctcgaact ctgcgttgaa
     3481 gttggcggca gcatcagtgg ggaacatggc atcgggcgag aaaaaatcaa tcaaatgtgc
     3541 gcccagttca atagcgatga aatcacgacc ttccatgcgg tcaaggcggc gtttgacccc
     3601 gatggtttgc tgaaccctgg gaaaaacatt cccacgctac accgctgtgc tgaatttggt
     3661 gccatgcatg tgcatcacgg tcatttacct tttcctgaac tggagcgctt ctgatgctac
     3721 gcgagtgtga ttacagtcag gcgctgctgg agcaggtgaa tcaggcaatt agcgataaaa
     3781 cgccgctggt gattcagggc agcaatagca aagccttttt aggtcgccca gtcaccgggc
     3841 aaacgctgga tgtgcgttgt catcgcggca ttgttaatta cgacccgacc gagctggtga
     3901 taaccgctcg tgccggaacg ccgctggtgg cgattgaagc ggcgctggag agcgcagggc
     3961 aaatgcttcc ctgcgagccg ccgcattatg gtgaagaagc cacctggggc gggatggtcg
     4021 cctgcgggct ggcggggccg cgtcgcccgt ggagcggttc ggtccgcgat tttgtcctcg
     4081 gcacgcgcat cattaccggc actggaaaac atctgcgttt tggtggcgaa gtgatgaaaa
     4141 acgttgccgg atacgatctc tcacggttaa tggccggaag ctacggttgt cttggcgtgc
     4201 tcactgaaat ctcaatgaaa gtgttaccgc gaccgcgcgc ctccctgagc ctgcgtcggg
     4261 aaatcagcct gcaagaagcc atgagtgaaa tcgccgagtg gcaactccag ccattaccca
     4321 ttagtggctt atgttacttc gacaatgcgt tgtggatccg ccttgagggc ggcgaaggat
     4381 cggtaaaagc agcgcgtgaa ctgctgggtg gcgaagaggt tgcgggtcag ttctggcagc
     4441 aattgcgtga acaacaactg ccgttcttct cgttaccagg taccttatgg cgcatttcat
     4501 tacccagcga tgcgccgatg atggatttac ccggcgagca actgatcgac tggggcgggg
     4561 cgttacgctg gctgaaatcg acagccgagg acaatcaaat ccatcgcatc gcccgcaacg
     4621 ctggcggtca tgcgacccgc tttagtgccg gagatggtgg ctttgccccg ctatcggctc
     4681 ctttattccg ctatcaccag cagcttaaac agcagctcga cccttgcggc gtgtttaacc
     4741 ccggtcgcat gtacgcggaa ctttgaggag caggctatgc aaacccaatt aactgaagag
     4801 atgcggcaga acgcgcgcgc gctggaagcc gacagcatcc tgcgcgcctg tgttcactgc
     4861 ggattttgta ccgcaacctg cccaacctat cagcttctgg gcgatgaact ggacgggccg
     4921 cgcgggcgca tctatctgat taaacaggtg ctggaaggca acgaagtcac gcttaaaaca
     4981 caggagcatc tcgatcgctg cctcacttgc cgcaattgtg aaaccacctg tccttctggt
     5041 gtgcgctatc acaatttgct ggatatcggg cgtgatattg tcgagcagaa agtgaaacgc
     5101 ccactgccgg agcgaatact gcgcgaagga ttgcgccagg tagtgccgcg tccggcggtc
     5161 ttccgtgcgc tgacgcaggt agggctggtg ctgcgaccgt ttttaccgga acaggtcaga
     5221 gcaaaactgc ctgctgaaac ggtgaaagct aaaccgcgtc cgccgctgcg ccataagcgt
     5281 cgggttttaa tgctggaagg ctgcgcccag cctacgcttt cgcccaacac caacgcggca
     5341 actgcgcgag tgctggatcg tctggggatc agcatcatgc cagctaacga agcaggctgt
     5401 tgtggcgcgg tggactatca tcttaatgcg caagagaaag ggttagcgcg ggcgcgaaat
     5461 aatattgatg cctggtggcc cgcgattgaa gcaggggccg aagcaatttt gcaaaccgcc
     5521 agcggctgcg gtgcgtttgt caaagagtac gggcagatgc tgaaaaacga tgcgttatat
     5581 gccgataaag cgcgtcaggt cagtgaactg gcggtcgatt tagtcgagct tctgcgcgag
     5641 gaaccgctgg aaaaactggc aattcgcggc gataaaaaac tggccttcca ctgcccgtgt
     5701 accctacaac atgcgcaaaa gctgaacggc gaagtggaaa aagtgttgct tcgtcttgga
     5761 tttaccttaa cggacgttcc cgacagccat ctgtgctgtg gttcagcggg aacatatgcg
     5821 ttaacgcatc ccgatctggc acgccagctg cgggataaca aaatgaatgc gctggaaagc
     5881 ggcaaaccgg aaatgatcgt caccgccaac attggttgcc agacgcatct ggcgagcgcc
     5941 ggtcgtacct ctgtgcgtca ctggattgaa attgtagaac aagcccttga aaaggaataa
     6001 caaaatgaaa actaaagtca ttcttagcca gcaaatggcg agtgcaatta ttgccgcagg
     6061 tcaggaagag gcgcagaaaa ataactggtc tgtttccatt gctgttgccg atgacggcgg
     6121 tcatctgctg gcgttaagtc gcatggacga ttgtgcgccg attgcggctt acatctccca
     6181 ggagaaagcg cgtaccgccg cgctggggcg tcgtgaaact aagggctatg aagagatggt
     6241 gaacaacgga cgtaccgcgt tcgtgactgc gccgttactg acgtcgctgg aaggcggcgt
     6301 accggttgtt gtggatgggc aaattattgg tgctgtgggc gtttctggtt taaccggagc
     6361 acaggatgcg caggtcgcga aagcggcagc agcggtgttg gcgaaataag cgaaaacgag
     6421 gagagaaaca atgagtcaaa ccataaccca gggccgttta cgcattgacg ccaattttaa
     6481 acgttttgtg gatgaagaag ttttaccggg aacagggttg gacgctgcgg cgttctggcg
     6541 caattttgat gagatcgttc acgatctggc accagaaaat cgtcagttgc tggcagaacg
     6601 cgatcgcatt caggcggcgc ttgatgagtg gcatcgcagc aatccggggc cggtaaaaga
     6661 taaagcggcc tataaatctt tcctgcgtga actgggctac ctggtgccgc aaccggagcg
     6721 cgtgacggtg gaaaccacgg gcattgacag cgaaatcacc agtcaggcgg ggccacagct
     6781 ggtggttccg gcaatgaacg cccgctacgc gctgaacgcg gcgaacgctc gctggggctc
     6841 actgtacgat gcgttatacg gtagcgacat catcccgcag gaaggggcga tggtcagcgg
     6901 ctacgatccg caacgcggtg agcaggttat cgcctgggtt cggcgtttcc tcgatgaatc
     6961 actaccgctg gaaaacggca gctatcagga tgtggtggcg tttaaggtgg ttgataaaca
     7021 attacgcatc cagttgaaaa atgataaaga aaccacgtta cgtaccccgg cgcagtttgt
     7081 cggttaccgt ggcaatgccg ctgcgccgtc ctgcattttg ctgaaaaata acggcctgca
     7141 tattgagctg caaatcgatg ccaacggacg gattggcaaa gacgatccgg cgcatatcaa
     7201 cgatgttatc gtcgaagcgg ccatcagtac cattctcgac tgcgaagatt cggtcgcggc
     7261 ggttgatgcg gaagataaaa tcctgctgta ccgcaacctg ctgggcctga tgcaggggac
     7321 tctgcaagag aaaatggaga aaaacggtcg gcaaatcgta cgtaaactga atgacgatcg
     7381 tcagtacacc gccgccgatg gctctgaaat ttctctgcac ggacgctcgc tgctgtttat
     7441 ccgcaacgtg ggtcatttga tgaccattcc tgtgatttgg gacagcgaag gcaatgaaat
     7501 cccggaaggc attcttgatg gcgtcatgac tggcgcgatt gccctctatg atttaaaagt
     7561 gcagaaaaac tcgcgcactg gcagcgtcta tattgtgaaa ccgaaaatgc acggtccgca
     7621 ggaagtggcg ttcgccaaca aactgtttac ccgcattgag acaatgctcg gtatggcacc
     7681 gaataccctg aaaatgggca ttatggatga agaacgtcgg acctcgctga acttgcgtag
     7741 ctgtatcgct caggcgcgca accgcgtggc gttcatcaat accggtttcc tcgaccgtac
     7801 cggcgatgaa atgcattcgg tgatggaagc tggcccgatg ctgcgtaaaa accagatgaa
     7861 atcgacgccg tggatcaaag cctacgaacg taataacgtg ctttccggtc tgttctgtgg
     7921 gctgcgcggt aaagcgcaaa ttggtaaagg catgtgggca atgccggacc tgatggcaga
     7981 catgtacagc cagaagggcg accaactgcg tgccggggca aacacagcct gggttccgtc
     8041 accaaccgct gctacgctcc atgcgctgca ctaccaccaa accaacgtac agagcgtgca
     8101 agccaacatt gcccagaccg agttcaatgc tgaatttgaa ccgctgctgg acgatctgct
     8161 gactattccg gttgctgaaa acgctaactg gtcggcgcaa gagatccaac aagagctgga
     8221 taacaacgtg caggggattc tggggtacgt ggtgcgctgg gtggagcagg ggattggttg
     8281 ttcaaaagtg ccggatattc acaatgtggc gctgatggaa gaccgcgcaa cgctgcgtat
     8341 ctccagccag catatcgcca actggttacg tcacggtatt ctgaccaaag aacaggtgca
     8401 ggcgtcgctg gagaatatgg cgaaagtggt tgatcagcaa aacgctggcg atccggctta
     8461 tcgtccgatg gcggggaatt tcgctaactc gtgtgctttt aaagctgcca gcgatttgat
     8521 cttcctcggc gtgaaacagc caaacggtta taccgaaccg ttattacacg cctggcgttt
     8581 acgcgaaaaa gaaagtcatt aatagcgtca aaaaagcccc ggcaagtttt attgtcgggg
     8641 ctatgatagt aactgcggta gtacatttat cccggaaata accggagcga ttatcagggt
     8701 ttaacaatat taaatacccg tagcgcatta ctagcgggcc taaaacaaca atatggacat
     8761 cttaacgata ctctgcgcct gcgtgtgcgg aacagaaacc cttacccgat atacttgtta
     8821 tgccagaggt agcaattgcg ttagcggcgt actgttgctg taaagtataa cgcctatatt
     8881 ttacagttca gtcctctttt gcttattaca accttaagta tatcaagcat ataaagataa
     8941 taagagactg aacaatatgg ttacctggac ccaaatgtat atgccgatgg gaggactggg
     9001 gctatccgct ctggtcgccc tgatcccgat aatattcttc ttcgttgcac tcgcggtatt
     9061 acgtctgaaa ggacatgttg ccggagcaat aacccttata ttatctatcc tgattgcgat
     9121 attcgccttt aaaatgccga ttgatatggc atttgctgct gcgggctatg gctttattta
     9181 tggattatgg ccaatagcgt ggattattgt cgcggcggtg ttcctgtata aattaaccgt
     9241 tgccagcggg cagttcgata ttatccgcag ctcggttatc tccatcaccg acgatcagcg
     9301 tttgcaggtg ttactgattg gtttctcctt tggtgcgttg ctggaaggag cggctggctt
     9361 tggtgcgccg gtggcgatta ccggtgcgct gctggtgggc ctgggcttca aaccgttata
     9421 cgcggcgggg ctgtgtctga ttgccaatac tgcgccagtg gcgtttggtg cgttgggcgt
     9481 gccgattctg gtcgccggtc aggtaacggg aatcgatccg ttccacattg gcgcaatggc
     9541 gggacgtcag ttaccgttcc tgtcggttct tgtgccgttc tggctggtag caatgatgga
     9601 cggctggaaa ggggtgaaag agacgtggcc agcggcgctg gttgcagggg gaagcttcgc
     9661 tgtcactcag ttctttacct ctaactatat tggtccggaa ctgccggata ttacttcggc
     9721 gctggtgagt atcgtctcac tcgctttatt ccttaaagtc tggcggccga aaaataccga
     9781 aacggcaatc agcatgggac aatccgcagg tgcgatggtg gtaaataagc catcttctgg
     9841 cggtcccgtg ccttcagaat atagtctggg gcaaatcatt cgagcgtggt caccgttttt
     9901 aatcttaacg gtgctggtca ccatctggac catgaagccg tttaaagcgt tatttgctcc
     9961 gggcggcgcg ttttattcac tggtgattaa tttccagatc cctcatttgc atcaacaagt
    10021 gttgaaagcg gcacccattg tcgcccaacc aacgccaatg gatgcggtgt ttaaattcga
    10081 ccccctctcg gctggcggca ccgctatttt tattgcggcg attatctcta tcttcatcct
    10141 cggcgtgggc atcaagaaag ggattggcgt ctttgccgaa acgctaatta gcttgaagtg
    10201 gccgatattg tcgattggca tggtgctggc gttcgctttc gtcaccaact attccggcat
    10261 gtccaccacg ctggcgctgg tactggcagg tacaggcgtg atgttcccgt tcttctcacc
    10321 gttcctcggc tggctgggcg tattccttac cggctcggac acctcctcta acgccctgtt
    10381 tggttcactg caatcgacca cggcgcagca aatcaacgtc tctgacactc tgctggtggc
    10441 agcaaatacc agcggcggcg taactggcaa gatgatctcc ccgcaatcta tcgccgtggc
    10501 ctgcgccgcg acgggcatgg tgggccgaga atctgaactg ttccgctaca ccgtgaagca
    10561 cagtctgatt tttgccagcg ttatcggcat tatcacgctg ttgcaggcgt atgtgtttac
    10621 cgggatgtta gtctcgtaaa tcaataaagc cggatgatcg tcaatatcat ccggcttcat
    10681 ttacattatt ctgtcttatt aattgaaaat catttctcgc tttattatcc atgcagcaat
    10741 aatgttttgc gttcagataa cctgctaaat aattaagagc gtgagaacat ctattttttt
    10801 atcacgcaaa acaagtctgt attaattatg ttcgcttgat gagcaaacac cgttctatct
    10861 gttgcttttg caatcttaat cgttaaaact accaacgaat ttatatagtt acgttgtgaa
    10921 atgttgttaa ttttttgttt at
//
LOCUS       DAZFFG010000136        10916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFFG010000136 DAZFFG010000000
VERSION     DAZFFG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.136"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            248..1147
                     /gene="lysO"
                     /locus_tag="ACHJ7J_004194"
     CDS             248..1147
                     /gene="lysO"
                     /locus_tag="ACHJ7J_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO5002357.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1291..2943
                     /gene="hcp"
                     /locus_tag="ACHJ7J_004195"
                     /gene_synonym="priS"
     CDS             1291..2943
                     /gene="hcp"
                     /locus_tag="ACHJ7J_004195"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO5002358.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LTVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2955..3923
                     /gene="hcr"
                     /locus_tag="ACHJ7J_004196"
     CDS             2955..3923
                     /gene="hcr"
                     /locus_tag="ACHJ7J_004196"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO5002359.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4056..5774
                     /gene="poxB"
                     /locus_tag="ACHJ7J_004197"
     CDS             4056..5774
                     /gene="poxB"
                     /locus_tag="ACHJ7J_004197"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO5002360.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCVGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5811..6812
                     /gene="ltaE"
                     /locus_tag="ACHJ7J_004198"
     CDS             5811..6812
                     /gene="ltaE"
                     /locus_tag="ACHJ7J_004198"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO5002361.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLRKAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6823..8253
                     /gene="ybjT"
                     /locus_tag="ACHJ7J_004199"
     CDS             6823..8253
                     /gene="ybjT"
                     /locus_tag="ACHJ7J_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO5002362.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGESGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISMWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLSLLFGMKAPGLGRLCFTLQDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8352..9365
                     /gene="ybjS"
                     /locus_tag="ACHJ7J_004200"
     CDS             8352..9365
                     /gene="ybjS"
                     /locus_tag="ACHJ7J_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5002363.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9362..10192)
                     /gene="amiD"
                     /locus_tag="ACHJ7J_004201"
     CDS             complement(9362..10192)
                     /gene="amiD"
                     /locus_tag="ACHJ7J_004201"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO5002364.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTTSL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10189..10512)
                     /gene="ybjQ"
                     /locus_tag="ACHJ7J_004202"
     CDS             complement(10189..10512)
                     /gene="ybjQ"
                     /locus_tag="ACHJ7J_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO5002365.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10723..>10916)
                     /locus_tag="ACHJ7J_004203"
     CDS             complement(10723..>10916)
                     /locus_tag="ACHJ7J_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5002366.1"
                     /translation="DRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQA
                     QDGEDFRNHLQYKLTLPTH"
BASE COUNT         2476 a         2905 c         2895 g         2640 t
ORIGIN      
        1 aaaacgcgcc ctcagcccac atccatgtgg gctgccccgg ccttcaggga acgcctcggc
       61 gattttgacg ccaccaacac cggtgcggtt tgtaattaaa gagcaagaat atctttaaat
      121 gaaaatgaat accctaccga tccttatgca taacatttcc ttttaattgc aactttactc
      181 gtccttccgc tttcagtagt gtgtcatcgc gcatttcgac ttctttcttt gcaagggctg
      241 ggttttcatg ttttctgggt tgttaatcat tctggttccc ctgattgtgg gttacctcat
      301 tccgcttcgc caacaagctg cgttaaaagt tattaatcag ctactaagct ggatggttta
      361 ccttattctc ttttttatgg gtatcagtct ggcgtttctc gataacctcg ccagtaacct
      421 gttggcgatt ctgcattatt ctgccgtcag tattaccgtt attttactgt gtaatattgc
      481 cgcccttatg tggctggagc gcggtctgcc gtggcgcaac caccatcagc aagaaaaact
      541 cccgtcgcgt attgcgatgg cgcttgagtc gctaaaactg tgcggcgtag tagtgattgg
      601 ttttgccatt ggcctgagtg gactggcttt cttacaacac gcgaccgaag ccagtgaata
      661 caccttaatt ctgctacttt tcctcgtcgg tattcagttg cgcaataatg gcatgacctt
      721 aaagcagatt gtccttaatc gccggggaat gattgtcgcc gtggtggtgg ttgccagttc
      781 attaattggt ggtttaatta acgcctttat tcttgatcta cccatcaata ccgcgctggc
      841 aatggcctct ggtttcggct ggtattctct ttccggtatt ttattgaccg aatcttttgg
      901 tccggtaatc gggagcgcgg cgttttttaa tgatctggcc cgtgagctga ttgcaattat
      961 gttgatccct gggctgattc gccgcagccg ctctaccgca ctgggcttat gcggtgcgac
     1021 gtcgatggat ttcaccctgc ccgttcttca acgtactggc gggctggata tggtcccggc
     1081 ggcaattgtt cacggtttta ttcttagcct gttagtgccg atcctcatcg cctttttctc
     1141 tgcgtaatac ctctctggcg gtagatccct gccgccaaaa ttgcgctaaa tcaatctccc
     1201 ttaaagttgc atgaaaaatc ccttttatcc ccgcgttaag cgtcttaacc ttaaacatgt
     1261 atattaaata taactttaaa aggtgtgatc atgttttgtg tgcaatgtga acaaactatc
     1321 cgtactccgg caggaaacgg ctgctcatac gcgcagggga tgtgtggtaa aacggcggaa
     1381 acctctgacc ttcaggattt actcatcgcg gcgctgcaag ggctttctgc ctgggcggta
     1441 aaagcgcgtg aatacggcat catcaaccac gatgtcgaca gcttcgcgcc acgtgccttt
     1501 ttctcaaccc tgaccaacgt taacttcgat tctccgcgta ttgtcggcta cgctcgtgaa
     1561 gcgattgcct tgcgcgaggc gctgaaagca caatgcctga ctgtagatgc caacgcccgc
     1621 gtcgataacc cgatggctga cctgcaactg gtgagcgacg atctcggcga actgcaacgt
     1681 caggcagcag aatttacccc taacaaagat aaagcggcga ttggcgaaaa cattctcggc
     1741 ctgcgtctgc tgtgcctgta tggcctgaaa ggtgcggcgg cctatatgga acacgcacac
     1801 gttctcggtc aatacgacaa tgatatttat gcccagtacc ataaaatcat ggcgtggctg
     1861 gggacctggc ctgccgatat gaacgcgctt cttgagtgtt caatggaaat cggccagatg
     1921 aacttcaaag tgatgagcat tctggatgca ggcgaaaccg gtaaatacgg tcacccgacg
     1981 ccaacccagg tcaacgtcaa agcgacggcg ggtaaatgca ttctgatttc cggtcacgat
     2041 ctcaaagatc tctacaacct tctggaacag accgaaggca cgggtgttaa tgtctacacc
     2101 cacggcgaaa tgctgcctgc gcatggctac ccggagctgc gtaaattcaa gcatctggtc
     2161 ggtaactacg gcagcggctg gcagaatcag caagtggagt tcgctcgttt ccctggcccc
     2221 atcgtgatga cctcgaactg catcatcgac ccaaccgtag gcgcttatga cgatcgtatc
     2281 tggacccgca gcatcgttgg ctggccgggt gtgcgtcatc tggatggcga agatttttct
     2341 gcggtcatcg cccaggcgca acaaatggca ggcttcccat acagcgaaat tccgcacctg
     2401 atcaccgtgg gctttggccg ccagacgctg cttggcgcag cggatacgct gattgatctg
     2461 gtgagccgtg aaaaactgcg tcatatcttc ctgcttggtg gctgtgacgg cgcacgcggc
     2521 gaacgtcact acttcaccga tttcgccacc agcgtgccgg atgactgcct gatcctgact
     2581 ctcgcctgtg gtaaatatcg ctttaacaaa cttgagtttg gcgatatcga aggtctgcct
     2641 cgtctggtag atgccggtca gtgtaacgat gcttactcag caattattct ggctgtcact
     2701 ctggcagaga aactgggctg tggcgtgaac gatctgccgc tgtcgctggt gctctcctgg
     2761 tttgaacaga aagcgattgt cattctgctg acgctgcttt ctctgggcgt gaaaaatatc
     2821 gtcaccggcc cgactgcacc tggtttcctg acaccagacc tgctggcggt gctgaacgag
     2881 aaattcggcc tgcgttctat caccactgtt gaagaagaca tgaagcaact gttgagcgcg
     2941 taaggagttt gtcgatgacg atgccaacga atcaatgccc gtggcggatg caggttcatc
     3001 acattaagca agaaacgccg gatgtgtgga cgatttccct gatttgccac gattactacc
     3061 catatcgcgc cgggcaatat gcactggtca gcgtgcgaaa ctcagcggaa acgctgcgtg
     3121 cttacactat ttcctccacg ccaggagtga gtgaatatat caccctgacc gtgcggcgga
     3181 ttgatgacgg tgtcggctcc cagtggctga cgcgcgatgt aaaacgcggt gattatctct
     3241 ggctttcgga cgcgatgggg gaatttacct gcgacgataa agcagaagat aaattcctgt
     3301 tgctggcggc aggctgcggc gtcacgccga ttatgtcgat gcgtcgctgg ctggcgaaga
     3361 atcgtccaca ggccgatgtg cgggtgatct acaacgtgcg tacgccgcag gatgttattt
     3421 tcgccgatga gtggcgtaac tatccggtaa cgctggtggc ggaaaataac gttaccgaag
     3481 gctttatcgc tggtcgtctc actcgcgaac tgctgactcg cgttcctgac ttagcttcac
     3541 gtaccgtgat gacttgtggt ccagcaccgt atatggattg ggtagagcag gaagtgaaag
     3601 cgctcggcgt gacgcgtttc tttaaagaga aattcttcac cccagtagcg gaagcggcga
     3661 ccagcggtct gaaattcacc aaactgcaac cggcacgaga attttacgcc ccggttggca
     3721 ctacgctact ggaggcgctg gaaagcaata acgttccggt tgtcgctgcc tgccgtgcgg
     3781 gtgtttgcgg ctgctgtaag acaaaagtgg tttccggtga atatacggtg agcagcacaa
     3841 tgacgctgac cgacgccgaa atcgctgaag gttacgtact ggcctgctcc tgccatccgc
     3901 agggggattt ggttctcgca taatcgcctt atgcccgatg atattcattt catcgggcta
     3961 tttaaccgtt agtgcctcct ttttctccca tcccttcccc ctccgtcaga tgaactaaac
     4021 ttgttaccgt tatcacattc aggagatgga gaaccatgaa acaaacggtt gcagcttata
     4081 tcgccaaaac actcgaatcg gcaggcgtga aacgcatctg gggagtcacg ggcgactctc
     4141 tgaacggtct tagcgacagt cttaatcgca tgggcaccat cgagtggatg tccacccgcc
     4201 atgaagaagt ggcggcgttt gccgctggcg ctgaagcaca actttcagga gaactggcgg
     4261 tctgtgccgg atcgtgcggc cccggcaacc tgcacttaat caacggcctg ttcgattgcc
     4321 accgcaatca cgttccggta ctggcgattg ccgctcatat tccctccagc gaaataggca
     4381 gcggctattt ccaggaaacc cacccacaag agctattccg cgaatgtagt cactattgcg
     4441 agctggtttc cagcccggag cagatcccac aagtgctggc gattgccatg cgcaaagcgg
     4501 tgcttaaccg tggcgtttcc gttgtcgtgt taccgggcga cgtggcgtta aaacctgcgc
     4561 cagaaggggc aaccacgcac tggtatcatg cgccacagcc agttgtgacg ccggaagaag
     4621 aagagttacg caaactggcg caactgctgc gttattccag caatatcgct ctgatgtgtg
     4681 gcagcggctg cgtgggggcg cataaagagt tagttgagtt tgccgggaaa attaaagccc
     4741 ctattgttca tgccctgcgc ggtaaagaac atgtcgaata cgataatccg tatgatgtcg
     4801 gaatgacggg attaatcggc ttctcgtcag gttttcacac tatgatgaat gccgatacgt
     4861 tagtgctgct cggcacgcaa tttccctacc gcgccttcta cccgaccgat gccaaaatca
     4921 ttcaaattga tatcaaccca gccagcatcg gcgctcacag caaggtggat atggcactgg
     4981 tcggcgatat caaatcgact ctgcgtgcat tgctgccact ggtggaagaa aaaaccgatc
     5041 gcaagtttct ggataaagcg ctggaagatt accgcgatgc ccgcaaaggg ctggacgatt
     5101 tagctaaacc gagcgagaaa gccattcacc cgcaatatct ggcgcagcaa attagtcatt
     5161 ttgccgccga tgacgccatt ttcacctgtg acgttggtac gccaacggtg tgggcagcgc
     5221 gttatctgaa aatgaacggc aagcgtcgcc tgttaggttc gtttaaccac ggttcgatgg
     5281 ctaacgccat gccgcaggcg ctgggcgcgc aggcaaccga gccggaacgt caggtggtcg
     5341 ccatgtgcgg cgatggcggt tttagcatgt tgatgggcga tttcctctcg gtagtgcaga
     5401 tgaaactgcc ggtgaaaatt atcgtcttta ataacagcgt gctgggcttt gtggcgatgg
     5461 agatgaaagc cggaggctac ctgacagacg gtactgagct gcacgacacc aactttgccc
     5521 gtatcgccga agcatgcggc attacgggta tccgtgtaga aaaagcgtct gaagttgatg
     5581 aagccctgca acgcgccttc tccatcgacg gtccggtgtt ggtggatgtg gtggtcgcca
     5641 aagaagagtt agccattcca ccgcagatca aacttgaaca ggccaaaggt ttcagcctgt
     5701 atatgctgcg cgcaatcatc agcggacgcg gtgatgaagt gatcgaactg gcgaaaacga
     5761 actggctaag gtaaaaaggg tggcatttcc cgtcataata aggacatgcc atgattgatt
     5821 tacgcagtga taccgttacc cgaccgagcc gcgccatgct cgaagcgatg atggccgccc
     5881 cggttgggga cgacgtttac ggagacgacc ctaccgttaa tgctttgcag gactatgcag
     5941 cagagctttc cggtaaagaa gccgccattt ttctgcctac cggcactcag gccaacctgg
     6001 tcgctctgct cagtcactgc gaacgtggcg aagagtatat tgtcggtcaa gccgcgcata
     6061 actatctgtt tgaagccggt ggcgcggcgg tgctgggcag tattcaaccg caacccatcg
     6121 acgcggctgc cgacggcacg ctaccgctgg ataaagtggc gatgaaaatc aaacccgacg
     6181 atatccattt cgcccgcacc aaattactca gtctggaaaa cacccacaac ggcaaagtgc
     6241 tgccgcgtga atacctgaaa gaagcatggg aatttacccg cgagcgcaat ctggcgctgc
     6301 atgttgacgg tgcgcgcatc tttaatgccg tggtggctta cggctgcgaa ctgaaagaga
     6361 tcacgcaata ttgtgattcg ttcaccattt gcctgtcgaa aggtcttggg acgccagtcg
     6421 gttcattact cgtcggtaat cgtgattaca ttaaacgtgc cattcgctgg cggaaaatgg
     6481 ccggtggcgg gatgcgccag tccggcattc tggctgccgc cgggatatat gccctgaaaa
     6541 ataatgttgc gcgcttgcag gaagaccacg acaacgctgc ctggatggcg gagcagctgc
     6601 gtaaagcagg cgcggatgtg atgcgtcagg acactaatat gctgtttgtt cgcgtcgggg
     6661 aagaaaatgc tgccgcgtta ggcgaataca tgaaagcgag aaacgtgctg attaacgcct
     6721 cgccgattgt ccgcctggtg acgcatcttg acgtctcgcg cgaacagctg gcggaagtcg
     6781 ccgcccactg gcgcgcattc ctggcgcgtt aaggagagaa acgtgccgca acgcatttta
     6841 gttctcggtg ccagtggcta cattggtcag catctggtgc gcacactcag ccagcaaggg
     6901 catcagatcc tggcggcggc acgtcatgtc gacaggcttg caaagctgca actggcaaac
     6961 gtcagttgcc ataaagtcga tctcagctgg ccggataacc ttccggccct gttacaggat
     7021 atcgataccg tctattttct ggtgcacagc atgggcgaaa gcggcgattt tatcgctcag
     7081 gagcgccagg tggctctcaa cgtccgcgat gcgctacgtg aagtaccagt taagcaatta
     7141 atctttctca gttcgttaca ggccccgcca catgagcagt cggatcatct gcgtgctcgt
     7201 caggctacgg cggatattct tcgtgaagca ggtgtcccgg tgaccgaact gcgggccgga
     7261 attatcgttg gcgcaggttc agcggcgttc gaagtcatgc gcgatatggt ctacaatctg
     7321 ccagtattaa cgccgccacg ctgggttcgt tcacgcacca cgcccatcgc gctggaaaac
     7381 ttgctgcact atctggtggc gctgttagat catccggcca gcgaacaccg catcttcgaa
     7441 gccgccggac cagaggtgct cagttatcag caacagtttg aacattttat ggcggtgagc
     7501 ggtaagcgcc gctggttgat ccccatcccc ctccccactc gctggatttc gatgtggttt
     7561 cttaatgtga ttacttccgt accgcccacc accgccaggg cgttgattca ggggctgaaa
     7621 cacgatctgc tggcggatga cacagcgcta cgtgcactca tcccacaacg gctgatcgct
     7681 ttcgatgacg cggtacgtag cacgttgaaa gaggaagaaa aactggtcaa ctccagcgac
     7741 tggggctacg acgctcaggc ctttgcccgc tggcgacctg agtacggtta tttcgccaaa
     7801 caggcggggt ttaccgttaa aacgtccgcc agccttgcgg ctttatggca ggtagtgaac
     7861 caaatcggcg gtaaagagcg ttatttcttt ggcaatattt tgtggcagac acgggcgttg
     7921 atggaccgtg cgatcggtca taaattagcg aaaggccgtc cggagcgcga atatttgcaa
     7981 actggcgatg cggtggatag ctggaaagtc attgtcgttg aaccgaaaaa gcaactttcg
     8041 ttgttatttg gcatgaaagc accggggctg ggacgactgt gttttaccct gcaagataaa
     8101 ggcgactatc gtactatcga tgtccgcgct ttctggcatc cgcacggtat gccggggctg
     8161 ttttactggt tattgatgat ccccgcgcat ctgtttattt ttcgcggaat ggcaaaacaa
     8221 atcgccagac tggcagaaca aagcacagat taatcgacaa aacctgcgtt attctttcat
     8281 gtttcccatt gcatcatggg ggaaatcacg gaagaatgcg cacgtcattt tctcaacaac
     8341 agtggattga tatgaaggta ctggttaccg gcgccaccag cggcttaggt cgaaacgcgg
     8401 tagagttttt atgccagaaa ggcatcagcg tgcgagcgac cggtcgcaac gaggcaatgg
     8461 gtaaattgct ggaaaaaatg ggcgcagagt ttgttccggc ggatctgacc gagctggtct
     8521 catcacaagc taaagtgatg ctcgcgggca ttgatacgct gtggcactgc tccagcttta
     8581 cctcaccctg ggggacacaa caggctttcg atctggctaa cgttcgcgcc actcgccgcc
     8641 tgggtgaatg ggctgtcgcc tggggtgtgc gtaactttat tcatatctct tccccctccc
     8701 tgtacttcga ttatcaccac catcgcgata ttaaagaaga ttttcgccct caccgcttcg
     8761 ccaacgagtt tgcccgcagc aaagcagcca gcgaagaggt gatcaatatg ctttcgcagg
     8821 cgaatccaca aacgcgcttt actattctgc gcccacaaag tctgttcgga ccgcacgata
     8881 aagtctttat cccccgtctg gcgcatatga tgcaccacta cggcagcatt ctgttaccgc
     8941 acggcggaag tgcgctggtc gatatgacct actatgaaaa tgccgtgcac gccatgtggc
     9001 tggcaagcca ggaagcctgc gataagctac cttccgggcg tgtgtacaac attaccaacg
     9061 gcgagcatcg cacactgcgc agcatcgtgc agaagctgat cgacgagttg aatattgact
     9121 gtcgtattcg ttccgtcccc tacccgatgc tggatatgat cgcccgcagc atggagcgtt
     9181 taggtcgcaa gtcagcaaaa gagccgccac tgacccacta cggcgtctcc aagttgaatt
     9241 ttgactttac gctggatatt acgcgtgcgc aggaagagtt aggttatcag ccggtcatca
     9301 ccctggatga aggtatcgag aaaactgccg cctggctgcg cgaccacgga aaactgccgc
     9361 gctaatcctg cccgtatttc tccagcaacg cttcggcaat cgcctgagtt tctgcatccg
     9421 cttcgccgtt atataacgtc gggcggaaat gcatctggaa cgccataatc acgcgccgct
     9481 gctcacgagg tgtcatatca ggttttacgt cataaccgta gcgcgccaaa agttccagca
     9541 atgacgtggt atctaccggt gtgtgcggcg cgcgcccggc aaggtaaaag ttcacccgct
     9601 gcgcatccgg ccaggcacca atcccctgct gcgccagttg ctgccaggga aataatggcc
     9661 ccggatcgtc tttgcgctgc ggcgcaatat ccgcatgtgc cactacgttt tccggcttga
     9721 tgtgataacg ggcaataata tctttcgcca gcggaataag cgcctgaatc tgtgccggtt
     9781 caaacggggc aaaatatttc acccccgccg atttttgcca gccacggttt tccagctcaa
     9841 tgccaataga ggtgtcgtta aggcgcgttg ccccgcgcca ggcgctaatc cccgcatgcc
     9901 aggccagttc ttgttccggc accaattgcc agatgcgcgg tttaccgttg tatcgcggtg
     9961 gcaccgcagg gaccagataa tgcgagctga cctgcttatc ggtcagtgtc gccagcgagc
    10021 tatcaaaatc atctgcggtg tagtggatca ccagcacttt aatgcgcgga tacgccgcct
    10081 gcgcctggcg tcgggtatca agctggtatc cctctttctc gacaatgcct ttttcgcctg
    10141 cacaccctgc cagtaacaga gcggcagcga ccagccagaa gactcttctc atcgacgcgt
    10201 tttcaccgcc gtaccgctga cgctaaccat cagcatactg ccgttttgcc cgaccgtttc
    10261 gtagtcgata tcaataccaa ctacggcatc ggcccccagc gcccgcgcct gggagcctaa
    10321 ttcctcaaag gcgatctccc gtgctttacg cagttctttt tcatacgcac cggaacgtcc
    10381 gccaacgata tcgcggatac cggcaaagaa atcacggaaa atattggcac ctaaaatcgc
    10441 ttcgccggtc accacaccgc aatattcaac gatggtctgg ccttccagag taggggttgt
    10501 agaaaattgc atgatcgatc tccttgtttg ttaagccttc gatctctaaa gcattatcgg
    10561 actgatatgc catgttggaa agaaggtttt gatcagttta ggaaatcatc tcattgtgtc
    10621 aaaagcagct acataagcct gtacaattat ctgtttcgta accggatatt ggtttcatta
    10681 tggagcaccg cttacctgtc ctattatgtt gtgccggcaa cattaatgag tgggaagagt
    10741 caacttatat tgtaaatgat tcctaaagtc ttccccatct tgcgcctgag gtatataccg
    10801 ctggattgct ttttggtccg ccgccgcaaa acaagaaagt aataagatct caatgctttt
    10861 gtcattaata taagcgaaga tcattcttgc aaattcgtat ataaacgccc gatcct
//
LOCUS       DAZFFG010000137        10787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFFG010000137 DAZFFG010000000
VERSION     DAZFFG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.137"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            160..591
                     /gene="osmC"
                     /locus_tag="ACHJ7J_004204"
     CDS             160..591
                     /gene="osmC"
                     /locus_tag="ACHJ7J_004204"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO5002367.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQLYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(647..1573)
                     /locus_tag="ACHJ7J_004205"
     CDS             complement(647..1573)
                     /locus_tag="ACHJ7J_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO5002368.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTELPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(1566..2552)
                     /locus_tag="ACHJ7J_004206"
     CDS             complement(1566..2552)
                     /locus_tag="ACHJ7J_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5002369.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRSEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIAMLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2549..3445)
                     /gene="ddpC"
                     /locus_tag="ACHJ7J_004207"
     CDS             complement(2549..3445)
                     /gene="ddpC"
                     /locus_tag="ACHJ7J_004207"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO5002370.1"
                     /translation="MMLSEETSAVRPAKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGVIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASCWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3442..4464)
                     /locus_tag="ACHJ7J_004208"
     CDS             complement(3442..4464)
                     /locus_tag="ACHJ7J_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5002371.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGDE"
     gene            complement(4466..6016)
                     /locus_tag="ACHJ7J_004209"
     CDS             complement(4466..6016)
                     /locus_tag="ACHJ7J_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5002372.1"
                     /translation="MKSSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPNDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRNYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(6030..6611)
                     /gene="ddpX"
                     /locus_tag="ACHJ7J_004210"
     CDS             complement(6030..6611)
                     /gene="ddpX"
                     /locus_tag="ACHJ7J_004210"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO5002373.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(6869..9268)
                     /gene="dosP"
                     /locus_tag="ACHJ7J_004211"
                     /gene_synonym="pdeO"
     CDS             complement(6869..9268)
                     /gene="dosP"
                     /locus_tag="ACHJ7J_004211"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO5002374.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(9293..10675)
                     /gene="dosC"
                     /locus_tag="ACHJ7J_004212"
     CDS             complement(9293..10675)
                     /gene="dosC"
                     /locus_tag="ACHJ7J_004212"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO5002375.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
BASE COUNT         2677 a         2784 c         2687 g         2639 t
ORIGIN      
        1 ctctcttgag atatatattc ccggtaatct attgtgggaa tttaatttaa gtgcagaagt
       61 aatattttct gccggatttt attcggaata tcctgcttat cctcgtgctg tttctcacgt
      121 agtctataat ttccttttta agcccacagg agagcaacaa tgacaatcca taagaaaggt
      181 caggcacact gggaaggcga tatcaaacgc gggaagggaa cagtatccac cgagagtggc
      241 gtgctgaacc aacagctgta tggatttaac acgcgttttg aaggcgaaaa aggaaccaac
      301 cctgaagaac tgattggcgc agcgcatgcc gcatgtttct caatggcgct ttcattaatg
      361 ctgggggaag cgggattcac gccaacatcg attgatacca ccgccgatgt gtcgctggat
      421 aaagtggatg ccggttttgc gattacgaaa atcgcactga agagtgaagt tgcggtgccg
      481 ggtattgatg cctctacctt tgacggcatc atccagaaag caaaagcagg atgcccggtc
      541 tctcaggtac tgaaagcgga aattacgctg gattaccagt tgaaatcgta aagcattgcc
      601 ggatgacgcg tcaggcgcat gaatgcctga tgcgttgtta gcatctctac agcgcccgcc
      661 agcaacggat ctcacgtccg tcagcccgcg tcgttaatga ttgccggact tcgcatccgc
      721 tggttgcaag ggggcaacgt tcgcggaaaa aacagccctg tggcaacgtg cggtttcctg
      781 gcagttccgt ttttcgtaat gcccattctt cctccagcgg tttatcaatg gcggggaggg
      841 aatccagcaa taatcgggtg tatggatgtg ccggagccgt tagcacctgt tgcgcttccc
      901 ctaattccac aatctgcccg agatacatca ccgctacccg atcgctcata tgacgaatca
      961 ccgagacatt gtgtgaaatc agcacataag tcagcccgtg attttcctgt agcgttacca
     1021 gtaaattgag gatctgcgcc tgcacggaga tatccagcgc cgaggttggc tcatcaagca
     1081 caatcacgtc aggctgcgaa gagagtgctc tggcaatggc gatgcgttgc cgctgcccgc
     1141 cggagaacgc atgaggcagg cggtcgagat actccggacg aatacccacc tgcacagcca
     1201 gctcctctgc caacgctcgc cgctgttgtt cactactacg cttagctatc cagagcggtt
     1261 ctgtgatgat gcgccacacc ggtaagcgcg gattgagcga agagagcggg tcctgaaaca
     1321 ccatctgcat aatgcgttgt gagccagaac ggatgtattg cccgtggctc ggttgcagca
     1381 tacccattaa aagctgtgcg agggtgcttt tgccgcagcc tgactcgccg acgatcccta
     1441 aggtttcacc acggcggatc tgtaaatcaa taccattaat ggcatgaaca tgttccgtag
     1501 ttttaccaag ccagttttta cgggccggga aattgatatg gacgtcgcgt aacgttaata
     1561 acgtgtcaga cacttatgac ctcctgctgc ggataccaac aggcgcagcg ctggttgttg
     1621 tcaccacacg ctgtcagcgc cgggacgttt tcacactgtg cacctgccgc atagcaacga
     1681 tcgcgaaaag cgcagccgtc aggcaaatgg gtgaggtttg gtaccgtccc tggaatggcg
     1741 ggtaatggct ggcgtggtac tccatgttct ggtgcgcatt gcagcaaacc aatggtatac
     1801 ggatgccggg gatgatggat aacgtctgcc gtcccgccgc tttcaattac gcttccggca
     1861 tacatcacgt aaacgctatc gcacagttgc gacaccacgg ccatatcatg gctgatgaac
     1921 agtaccgcag ttccactggc gcgggcttta tgtttaagca aacgcagtac ctgcaactgt
     1981 accgtgacgt ccagcgccgt agtcggttcg tcggcaataa tcaattgcgg ctcgcaggag
     2041 aatgccagcg caatcattac ccgctggcgc ataccacctg aaagctcaaa cggatagcgc
     2101 gacataactt ccacggcatc cgggatttgc atctcttcca gcatggcaat cgctttagct
     2161 ctggcttccc gacgacttat tggttgatga tggcggatca cgtccatcat ctgaagacct
     2221 attcgacgtg tcggattgag ggcggtcatc ggttcctgaa agatcatcgc cactcgtgcg
     2281 ccgcgccact gacgaagctg tttttcccga gcgttaagaa catcttctcc cagcagtgaa
     2341 atctgccccc gatgtacgca ataactgccc gtcggtagca gacgcataat cagcattgcg
     2401 gtgactgatt tacctgagcc ggattctccc accagaccga caatttcact gcggttaatc
     2461 tgcaaggaca cattgttgag cgcgtgaaca tcaccgttaa aaccggggaa actcaaatgc
     2521 agttgttgaa tgtccagaac gggttgggtc atgactgctt tcctcctgct ttcgggtcaa
     2581 gcagatcgcg aataccatca ccaaagagat taaacccgac ggcggtaagc aaaatcgctg
     2641 cccccggaaa tgcgcaatac caccactgat cgagtacata gttgcgacca tttgctacca
     2701 tcgcgcccca ttcagcactc ggttgttgag cacccaggcc aataaatccc aacgtggcgg
     2761 ccattaaaat cgcgctaccg atatccagcg atgcctgcac gatcagcggc ggtagggagt
     2821 tacgtaaaat atgccagttg atcagatgcc aacaagacgc gccaaaggtt ttcgccgcct
     2881 gaacataggt atactggcgt acaactaatg cttgcccccg cgccaggcgc acataaaagg
     2941 gaattcgcac aatagcaatc gccagcatgg cgttaaataa actcggcccg agagcggcag
     3001 ccagtgccat tgtcagtacc aacgaaggaa tcgacagcat aatgtccatg atgcgcatga
     3061 tgatggcgtc tgcgcgtccg ccaagcacac cggatagaca tccgagtagc gaaccaatca
     3121 cacccgcaat ggcgaccacc actaatccgg cgagaattga ttgctgactg ccgaccagta
     3181 cgcggctgaa cagatcgcgt cccacttcat cggtgccaaa ccagtgcgcc gcagaaggcg
     3241 gcaaaaggcg ggcggttaaa tcaatggcgt tgggatcatg cgtcgcaagc cacggtgaaa
     3301 aaatcatcat caatagcatt aatacaatga tgactgcgcc ggtcacggtg agcggactgc
     3361 ctttcagcat ccataccagt tttgcaccgt taaatcgcgt ttgttttgct gggcgtacgg
     3421 cggacgtttc ctcgcttagc atcattcatc acctccacgt ccgatacgcg gatcaatcca
     3481 caaatagagc aaatccacta ccaggttcac cagcacataa gcaaacgaaa ccacgacggc
     3541 aaatcccatc accgcaggga agtcgagcgc ctgtattgat gtcactaccc atgcccccat
     3601 tccgggccag gcaaaaacgg tttcggtgag cactgcgcca tacaacaaat cgcccagcgc
     3661 cagacccaat acggtaatcg atgggatcaa cgcattgggt agcgcataac ataaaacgat
     3721 ataccagccg ggcaagccgc tggcccgggc ggtacgaatg tagtcttcac tcaattgttc
     3781 cagcatcgct gagcggatct ggcgagcgac aattcccagg tgaacgaacg ccagcgttaa
     3841 tgccggtaag atgagatgtt gcaacgcatt gaagaagact tcaccgttgc cttcaagcag
     3901 cgcatcgagc agataaaagc cggtaacgtg cgttggtgga tccagccagt catcaagcct
     3961 tccgccgccg ggaagaattt gcagatgacc ataaaacagc acaatgacgc ccagtccaag
     4021 ccagaacgca ggtgtggaga ttccggtaat ggccattatt cgcaccagat gatccagcca
     4081 gcgatttcgc cagactgcag agagtatgcc cagcgggatg ccaatcaaga gtgccagcag
     4141 cagggcacaa aaagccagct ccagcgtcgc cgggaaaaat atacgcaact cttccagcac
     4201 cggacgcccg gtacgaatgg atgttcccag gtcaccatga aacaggtcgc tgacgtagcg
     4261 gtaaaactgt acatacagtg gctggtccag tcccagttgc tggcgaatat tttccacgat
     4321 agcatcgctg gcgcggtcac ccgccagtaa ccgcgccgga tcgccgggga tcagatgcga
     4381 gataataaac gtaatcacgc agacgcccgc caccacgagc accagccccc agcagcgttg
     4441 gcgtaaaata ctccagaagg tcacgttatt tactcatggt attgatattg aagacctgtt
     4501 ccagcatggg attgaacaca aagcctttca cctctttgtt catcgccagt tggtagtttt
     4561 tctggaacag gtacacataa gcagcgtcat caatgacgat tttctgtgcc tgctggtagt
     4621 tccgggtacg ctgcgtctgg tcggtggtcg cgagcgcatt gcgcagtaac ttatcgacct
     4681 cactgttttc atagaacgag cggttacccg gcagaccttt tttgtctgac tcaaaccagt
     4741 aattcataaa catatacggg tcggcaaaat ccggactcca gttgccaatc gcaatgtcgt
     4801 aatcaccttt acccactctg tcgcgcatgg tggcgttcgc cagcttttcc agcttcacat
     4861 tgatgcccag cttgttgagg ctggattgtg ttgccagagc gatcggttcc cagttcggat
     4921 cgttatcgga gtagagaaac gtcaggctgg tgggtttgct cgtcacttta tcccattcgg
     4981 ctttggcttt cgtttcgtca tggttgtatt gcattgccgt cgcatcgtag ccccacatgc
     5041 cttccggaat cgggccgcgc atttgttttc cgttaccact cagaatgccg ttaaccatgc
     5101 cctgataatc ggtagaccag gaaatagccc gccgcagatc cgcctgatta agtggcgctt
     5161 tgctgttatt aagatacaga taggtgacgc gcagtgacgg atactctgcc acattgactt
     5221 tgttttcctg cttcagggcg ttgagttgat ccaccggcag cgcatcggca atgtcaatgt
     5281 cgccacggga gagctgcagg cgacgggagg cactttcacc gataattttt accgatactc
     5341 gcttaaagtt cggtttattg ccggggtaat gcggatttgg caccagaact aattgctgac
     5401 ctttttgcca gcttttcagc ataaatggtc cggaaccggc ggtattttgc gcgaggaagc
     5461 cgcgggcatc atccgccgca tgttccttta acaccgccgg attgataatg gatgcaccgt
     5521 cattcgccag cgtgtagagg aacggtgcaa atggctggct aagggtaaac ttcaccgtat
     5581 gttcgtcggg agcatcaatc tttaaatcat tgggaaatgc ttctgctggc ccctggccga
     5641 tttttagtaa ccgctcaaaa gagagtttta ctgcttcggc agtgacaggt gtgccatcgg
     5701 caaatttagc attatctttc agggtgaacg tccactcttt ttgatcgtta gacgctttcc
     5761 agctacttgc cagatcgcct tcaacgtcgg ttgagccttt atcaccgtcc gttttgtact
     5821 gaaccagtcg ctgataagac gggtaggtca ctgtccagtc gttattatct attgttaccg
     5881 ccgggtcgag ggtttgtgga tcggcggcct taccaatcac cagcatatct ttcggtacgg
     5941 cggcgtgcgc aaccgggaaa gttgtggcaa ggacgagcgc gagcaatgtg ggacgaaacg
     6001 atatcgagct cttcatgaca gtttcctgat taactgacgt gctgtgtgcc aggggatata
     6061 aaacaggtga attgatcagc gagcagaggg taactcgctg cctggggtaa ttcgaagtgc
     6121 caccattcgc tggagatacc gacaaaaccg ccaccagtca taatcgcatt cagcaacagc
     6181 cgattgcgct gagcggcggg cgggacggaa ggatggtaag catgggagcg ctcgtgcatt
     6241 tcatcgaacc ctgcgcccat atcgaggatg ttcccgtgct catcacgaag cgtcaggtcg
     6301 atcgccgtgc cacggctgtg attagaaccg accgtcacat caacaacata ttgcgggtct
     6361 gggcaggctt gccacaacat cgcctgtgct tgttgtgggc gatacgcatc gtaaatcacc
     6421 agttgtaagc ctgatagctg ggcgatactg atacttttcg ccagcgcggt aatcgcatcc
     6481 ttgtgtaaca gacaacgcgc ttgctggtaa atagctttac ctgtgatgtt atcagcgcag
     6541 gcgtatttca attcgatctc cagatcaggg aagatcacgg ctaaatcaac cagttcggtg
     6601 gtatccgaca taaacggttc ctgtataaga aaaaaattgc tgcgctttcc gcttatgcag
     6661 atctcatgcc atgccgggat aagcgccaga atgctggctt aaagttatat tttttaactt
     6721 ttgatcaaca tttgtgcagc gtagtgcagt tttggtgcaa gaggggaagt taaggaagga
     6781 atctcccgga atcgtagctg aaatcacagt atttaagtga cagtgtcacg ttaaatgaaa
     6841 acccgcgagt gcgggcgaga ggaatttgtc agattttcag cggtaacacg ctgctcatcc
     6901 agcctggaat ttcttcggcg ggtagggggc gggaaaagaa atatccctga ataacgcgac
     6961 agtggatctt gcgtagcatc tcaaattgct ctttggtttc gacgccttcc gccacgacgg
     7021 ttaaattgag gctttgccca atgctggtaa tggcttcaag taaggcaagg atgcgttttt
     7081 ccgtcagaca acgatcgaca aaacttttgt caattttgat ttccgttacc ggaagactga
     7141 ctaagcggga taatcctgaa aagcccgtgc caaaatcatc taccgataaa cccacgccca
     7201 tatcacgcag gatctgaatg cgcttaaaga tttcggtatc gtgttccatc atcatgcttt
     7261 ccgtgatttc taccgtcagc tggtggccgt caatacccca ggcttgcatt gcatcagaca
     7321 cctgattagg cagttgatta ctgcgaaagt gcagcgccga caagttcacg gataacgccg
     7381 ggatatgaat attctggcta cgccattctg ctaactgacg gcaagcttcc gcgatgaccc
     7441 agcgcccaat attttcgatt tcaccaatct cttctgcgag aggaataaac cgtgaagggg
     7501 gcacatgacc atgctgggga tcgtgccagc gagcaagggc ttcgatgccg tagagttcac
     7561 ccgtttctgc gaagatttgc ggctggtaaa ccagtttcag ttggttattg ctaatcgctt
     7621 ctttcagcgc tgcgcctaaa accaaacgct cttttaccat ttcgttcatc gccgggctga
     7681 agaactgcca gccgttaccg ccattcttgc gaatataatc cattgcattg tgagcagtgg
     7741 agagcaggta atcgcggttt ttacccacgt cgtagctgat gccaatactc aaggtaagcg
     7801 ggaagggttt atcgtcaatc attatcggct tgctgaccac attccgtagc tcatcggcga
     7861 tttgggtaat gttactgacg tcgttttcca ggctcacgag gacaaactgc gtaccttcga
     7921 tacgacagag atactgatcc ggtttgagtt tttcacgaaa gcgattgacc acttccagca
     7981 atgcctgatc ggcccacgca tagccaaggc tatcaatcac atcctgaata tggtcaacac
     8041 cgatgagata caccaccgga gagacggctt tgtcgaccag gtcatcgagg taattgtgca
     8101 ggttattgcg atttggcaga ccggtcatcg gatcaaattg gatgagttgt tcaatatgct
     8161 gacggctttt ttcctgttcc agcgccagcg cggccatatg ctggctgata tctgccacgc
     8221 gttcgataaa ggcgctggtt tctgctcctg acgaggtttt aatttgcagg atccccgcag
     8281 gcgcgccatc acgctgacga atggtcgctg accagctttg cgcattttga acttctgcac
     8341 cgtgcgaaga tgacgcccag tgtatcggca tcccgttgcg cagtgcgaac agcgaaacat
     8401 gcgattcgtt gagaacagat tcgatattac gacaaatgat ttcccccatt tcatgaaatg
     8461 gcgggctgct gcacatggcg gcgagaatat tgccttcaag ctggcggatt tgccgttctt
     8521 cggtgatatc cgagaaggtc attaccaggt tctgcagatg cgcgagcacg tcataaaccg
     8581 ggctgataga agctttaatc cagatttttt caccggtgcg cgtcaacagc agaaattcgt
     8641 cctgatcgcg ggcggttttc catagcaact gttgtaaacg aatgcggtta tcggcaggga
     8701 attcaggaat gttcaggagt gtatcgggct gcataccgct ggcttcgcta atgcagtaac
     8761 caaacatttc ggtaaatgcg cgattgcact gcacaatatg gcgttccgga tcgaggacaa
     8821 tcaccggtcg gtcgagatgg tcaacggcaa taatcaattg tctggtctgt tctttttgcg
     8881 ccatttctac gctggcatcc cgtaccagcg ccaggtaata aactttcccc tcggcgctca
     8941 ctttcgatag cgcaaaacgg gtccagattt tactgccgtc ttttttctcc agctgcagct
     9001 cccgactcat cccctcaaca cgcgctttac cgccttcacg gttatgacga atgtattcag
     9061 gatgcgcagg acgcaaatcc cgcggaatca gcatatcaat gttattgcca atgacttctt
     9121 cacgtttgta tccccagagc ttctctgcgg cggggttgaa aaacatcact tcatcatttt
     9181 cgttaattaa caccgcaccc atcatatttt gctcaagggc ggggaaaaaa atgccatcgg
     9241 cggcattatc cgcatcggtt agcttcatga ttacctctgc atcctggcgc atctaaagac
     9301 tggctttcca gagttcaaca cggtttctac ctcgtctttt ggcgatatac agagcttcat
     9361 cggctatttg aatgaggcgc tcatagtcag gatgaccatt aaacatggcg gcaccgatgg
     9421 aaagtgagag ggcaatatct tcgccgtttg cggctttcag tttggttttc tccacccgac
     9481 tgcgaatacg ttctgcggta cgtaacgttt cgttttcaga agcttcagtc aaaacaatga
     9541 taaattcatc gcccccgtag cggaaaacat aatcactact gcggacgttg tcataaaagg
     9601 cctgagagac tttacgcaga atttcatcgc cagtgttatg gccccacgta tcgttgatct
     9661 ctttgaattt atcaacgtca ataatcagca ctgacagcgg tgtaccggtc cggttggcat
     9721 gggcaatttc gcgtttgaag atagtcggca ggaaacggcg gttaagtaat ttcgtcagta
     9781 catccatacc gacttcgtgg cgcgatactt cttcaaacaa ttcacgcagc aaggtaataa
     9841 tttgcgatac ggtatttctt atctgtaata aaaatttcac ccgcagactt ctgttattca
     9901 aattcctggt gttacgcatg gtttgattga aaataccgtc gaaatcctga atcagacgag
     9961 agatatggcc cacttcagca atgccgctga aataatgtcg acctttatgg ttaaaccaca
    10021 ggccaaaatc agcctggctt aaaggcaaac tactgcctaa atcagaatcc agcaggattt
    10081 tatagatgat atctatttcc catgaaagta ttgaggctat ttgccgttct ttttcttctt
    10141 cggcgttttc cagtaacgag aagatacgat agttttcatc ttcttttgag gcactactgt
    10201 cactaaaggt aaacgcgcgg gtcatcactt ccatagcgat atcaatactg ttaatcgaga
    10261 aatggtagac ctgaagtttt tctgcggcag aataatccga agagaagatt accggataga
    10321 ggatcttttt cagcacccga aaccccatct cgacaatttc taccggaatt cctatgcggg
    10381 catgcacttc cgcgacggta tgctggattt gtattagcct ttcgacatcg tcaacctggg
    10441 cagaaagcac gttaataatc cagcgttcca tcgcactttt caactgccgc tcaacttgtt
    10501 cattactcaa gaattcttcg gcatgcgggt cgatgcggac aattcgataa aactcaatac
    10561 tcagataatg agcatgcgca acggcaattt ccgcggcttt agctcgaatg ggcggatctg
    10621 cctgttcgac aagtccggtc cactcatctt tcattctttt aaaatacatc tccataattc
    10681 acacccttat aaggctggga aatcagacgg aatcaaaacg aaacgcaacg tgcgagatcg
    10741 actaactgca ccatattctc ctgaaatatg aagatatact gaaaaga
//
LOCUS       DAZFFG010000138        10755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFFG010000138 DAZFFG010000000
VERSION     DAZFFG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.138"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(83..1129)
                     /gene="aroH"
                     /locus_tag="ACHJ7J_004213"
     CDS             complement(83..1129)
                     /gene="aroH"
                     /locus_tag="ACHJ7J_004213"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO5002376.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1286..2119)
                     /gene="ppsR"
                     /locus_tag="ACHJ7J_004214"
     CDS             complement(1286..2119)
                     /gene="ppsR"
                     /locus_tag="ACHJ7J_004214"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO5002377.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2452..4830
                     /gene="ppsA"
                     /locus_tag="ACHJ7J_004215"
     CDS             2452..4830
                     /gene="ppsA"
                     /locus_tag="ACHJ7J_004215"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO5002378.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQDQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4887..6533)
                     /gene="fadK"
                     /locus_tag="ACHJ7J_004216"
     CDS             complement(4887..6533)
                     /gene="fadK"
                     /locus_tag="ACHJ7J_004216"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO5002379.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6590..6883)
                     /locus_tag="ACHJ7J_004217"
     CDS             complement(6590..6883)
                     /locus_tag="ACHJ7J_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5002380.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDIKEFQKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(6880..8169)
                     /locus_tag="ACHJ7J_004218"
     CDS             complement(6880..8169)
                     /locus_tag="ACHJ7J_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5002381.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8225..9163)
                     /locus_tag="ACHJ7J_004219"
     CDS             complement(8225..9163)
                     /locus_tag="ACHJ7J_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO5002382.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     IPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9183..9947)
                     /locus_tag="ACHJ7J_004220"
     CDS             complement(9183..9947)
                     /locus_tag="ACHJ7J_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO5002383.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            10263..>10755
                     /locus_tag="ACHJ7J_004221"
     CDS             10263..>10755
                     /locus_tag="ACHJ7J_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO5002384.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYEN"
BASE COUNT         2594 a         2727 c         2788 g         2646 t
ORIGIN      
        1 ggctgaccag agcaagcagt aatgtgttga gaagacaaca agttgcgcag aaatgcccat
       61 cccatcagga atgggcacgc attcagaagc gggtatctac cgcagaggcg agtttttcga
      121 ccaggcgttc ggtatcttcc cagcccagac acgggtcggt aatggattga ccgtaagtga
      181 gcggctgacc gccgacgatt ttttgcgttc cttcgcgcag gaaactttcc gccataattc
      241 cagcaatcgc cgtagagcca ttgcggattt gctgacaaat atcctcacaa acttctaact
      301 ggcgacggtg ctgcttctgg cagttaccgt ggctgaaatc caccaccaga tgttcaggta
      361 aatcaaactc gtgcagcgta tcgcaggctg cggcgatatc atcggcatga taattcggtt
      421 ttttgccgcc acgcataata atgtggccat acgggttgcc gctggtctga tagatggtca
      481 tctgaccatt tttgtctggc gagaggaaca tatggctggc gcgggctgcg cggatagcat
      541 ccacagcaat ccgcgtattg ccatcggtac catttttaaa acctaccgga caggagagtg
      601 ccgaagccat ttcacggtgg atctgacttt cggtagtacg tgcgccaatc gcgccccaac
      661 tgattaaatc agcaataaac tgaccggtca ccatatcgag gaactcggtc gcggttggga
      721 cgcccagctc atttacctgt aaaagtaatt tgcgcgccag ctccagaccg tgatttaccc
      781 gatagctgcc gtttaaatct ggatcggaga ttagtccttt ccagccgaca acagttcgtg
      841 gtttttcaaa ataggtgcgc attacgattt ccagccgtga ctggtactgg ttgcgcagcg
      901 actgcagacg ggtggcgtac tccattgcag cggtgagatc gtggatcgag caggggccaa
      961 taatgaccaa cagtcgctta tcttcaccat tgagtatttt ttcaattctg cggcgggagt
     1021 cggtgacatg ggtggcgacg ccaggcgtta cgggataccg tagcgcgagt tcggcgggcg
     1081 ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt tctgttcatt acaagtctca
     1141 gaaatttgtt gcttctacgg caggactgcc gggaagtgat gcgcatcacg ataaaccaat
     1201 cgctgctgat ttcaagtgcg aggtgtgtca cacctcgccg ggtggttagc atgataacaa
     1261 aaaaataagc taatgcacta gttctctagt acattcggcg actaaggccc atgatatcga
     1321 ggatcttggt ggcaatctct tctaccgaat aattggtact gttaatccac gggatctgat
     1381 ttttacggta caaggcttcc acttccgcga cttccatcct gcactgacga agcgaggcat
     1441 agcgactgtt ctcccgacgt tcctcgcgaa tcgccgccag acgttccggg tcgatagtca
     1501 ggccgaacaa tttatgctga agcggtttga gcgacgcggg tagcaccaga ttatccatat
     1561 cgtcggcaat aaaggggtag tttgccgcgc ggataccaaa ttgcattgcc agatacagac
     1621 tggtgggggt tttaccacag cgagaaacac cgagcaggat cacctgagcc tgatccagat
     1681 tgcgcaacga aatgccgtca tcgtgggcga gggtgtaatc aatcgccgca atgcgcgcat
     1741 catatttatt gagattatta gggttaaggc catgggtacg atgagcaatc ggcgttggat
     1801 ccagtttcat ctcttgttgt agcggggcaa ccagcgcctg aacgatatcc tggcaaaaac
     1861 cttcactttg caagatgatg gcgcgaatct ccggcaacac gatggagtag aagaccagcg
     1921 ggcgcacgcc tgtctggtga taaattgcgt caatctgatc cttcactgcc cgtgcacggc
     1981 tctcattttc gacaaacggc agcgtaatgc tgctgatagt gacgggaaat tgtgacatta
     2041 ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc agaaatataa aaaacgtggc
     2101 gatcaacagc attatccatt ttgttcttcc cgtgatgcag acataattct atgaaagcat
     2161 aaattaaaaa cgcacagaag cgtagaacgt tatgtctggt ttataaaatg aaccttcaat
     2221 tttatttttt atgaaaacag catttcattt ttatggtttc gtttataccg atggtttatg
     2281 tggaaattgt cgaagagagc agatttgcgc aacgctggga tcagtcttaa aaagtaaaaa
     2341 aatatatttg cttgaacgat tcaccgtttt tttcatccgg ttaaatatgc aaagataaat
     2401 gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa aggattgttc gatgtccaac
     2461 aatggctcgt caccgctggt gctttggtat aaccaactcg gcatgaatga tgtagacagg
     2521 gttgggggca aaaatgcctc cctgggtgaa atgattacta acctttccgg aatgggtgtt
     2581 tccgttccga atggtttcgc cacaaccgcc gacgcgttta accagtttct ggaccaaagc
     2641 ggcgtaaacc agcgcattta tgaactgctg gataaaacgg atattgacga tgttactcag
     2701 cttgcgaaag cgggcgcgca aatccgccag tggattatcg acactccctt ccagcctgag
     2761 ctggaaaacg ccatccgcga agcctatgca cagctttccg ccgatgacga aaacgcctct
     2821 tttgcggtgc gctcctccgc caccgcagaa gatatgccgg acgcttcttt tgccggtcag
     2881 caggaaacct tcctcaatgt tcagggtttt gacgccgttc tcgtggcagt gaaacatgta
     2941 tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg ttacgatcac
     3001 cgtggtgtgg cgctctccgc cggtgttcaa cggatggtgc gctctgacct cgcatcatct
     3061 ggcgtgatgt tctccattga taccgaatcc ggctttgacc aggtggtgtt tatcacttcc
     3121 gcatggggcc ttggtgagat ggtcgtgcag ggtgcggtta acccggatga gttttacgtg
     3181 cataaaccga cactggcggc gaatcgcccg gctatcgtgc gccgcaccat ggggtcgaaa
     3241 aaaatccgca tggtttacgc gccgacccag gagcacggca agcaggttaa aatcgaagac
     3301 gtaccgcagg accagcgtga catcttctcg ctgaccaacg aagaagtgca ggaactggca
     3361 aaacaggccg tacaaattga gaaacactac ggtcgcccga tggatattga gtgggcgaaa
     3421 gatggccaca ccggcaaact gttcattgtg caggcgcgtc cggaaaccgt gcgctcacgc
     3481 ggtcaggtca tggagcgtta tacgctgcat tcacagggta agattatcgc cgaaggccgt
     3541 gctatcggtc atcgcatcgg tgcgggtccg gtgaaagtca tccatgacat cagcgaaatg
     3601 aaccgcatcg aacctggtga cgtgctggtc actgacatga ccgacccgga ctgggaaccg
     3661 atcatgaaga aagcatctgc catcgtcacc aaccgtggcg gtcgtacctg tcacgcggcg
     3721 atcatcgctc gtgaactggg cattccggcg gtagtgggct gtggtgatgc aacagaacgg
     3781 atgaaagacg gtgagaacgt cactgtttct tgtgccgaag gtgataccgg ttacgtctat
     3841 gcggagttgc tggaatttag cgtgaaaagc tccagcgtag aaacgatgcc ggatctgccg
     3901 ttgaaagtga tgatgaacgt cggtaacccg gaccgagctt tcgacttcgc ctgtctgccg
     3961 aacgaaggcg tgggacttgc gcgtctggaa tttatcatca accgtatgat tggcgtccac
     4021 ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga aatccgcgag
     4081 atgatgaaag gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac tgaagggatc
     4141 gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc tgattttaaa
     4201 tcgaacgaat atgccaacct ggtcggtggt gagcgttacg agccagatga agagaacccg
     4261 atgctcggct tccgtggcgc gggacgctat gtttccgaca gcttccgcga ctgtttcgcg
     4321 ctggagtgcg aagcagtgaa acgtgtgcgc aacgacatgg ggctgaccaa cgttgagatc
     4381 atgatcccgt tcgtgcgtac cgtagatcag gcgaaagcgg tggttgaaga actggcgcgt
     4441 caggggctga aacgtggcga gaacgggctg aaaatcatca tgatgtgtga aattccgtcc
     4501 aacgccttgc tggcagagca gttcctcgaa tatttcgacg gcttctcaat tggctcaaac
     4561 gatatgacgc agctggcgct cggtctggat cgtgactccg gcgtggtgtc tgaattgttc
     4621 gatgagcgca acgatgcggt gaaagcactg ctgtcgatgg cgattcgtgc cgcgaagaaa
     4681 cagggcaaat atgtcgggat ttgcggtcag ggtccgtccg accacgaaga ttttgccgca
     4741 tggttgatgg aagaggggat cgatagcctg tctctgaatc cggacaccgt ggtgcaaacc
     4801 tggttaagcc tggctgaact gaagaaataa aataaatacc cggcggcgtt ttgtcgccgg
     4861 ggttatgtga ccctcgaaga tgaaacttat tcaatctctt cacagacatc ctgcgttaat
     4921 cgccgcataa tatcttttct taacaaaaac ttttgtattt tacctgaggc agtgcgcggt
     4981 agtttttcga ttaccacgat atgttcagga tatttatatt ttgcgacccg tttacggcta
     5041 aaaaaagcca ccacctcttc cagcgataat gaatgatgcg gcgctttcag cacgacataa
     5101 gcgcatgatc gttcacctaa acgttcatcg ggcattgcaa ccacacaggc atcgtgaatt
     5161 ttaggatgct gcaataaaat atcttccact tcacggctgc taatattttc gccgccgcgg
     5221 acaataatat cttttttgcg tccggtaatt tttatatagc cagcctcatc catacggcag
     5281 agatcgccgc tgtaatacca gccttcttca tccagcgcac gggcggttaa ttcaggttca
     5341 tcaaaatatc ccataaacac attggggcca cgcgaggctt cttcaccttc gcaacctggc
     5401 ggtaaggtct tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc agcgtaacca
     5461 tcggtgtgca taaagcgcga caaaggatca tcgagattca ccaccgcatg cggcgaactt
     5521 tctgtggaac cataaacact taataattta atgccgcgct gctggcattc acgcgccact
     5581 tttttgggga ttgtggtacc gccgcaaaga aagaagcgca gcgctgaaag gtctgcgggt
     5641 tgtttctcta gtaaattcaa aagatcatag acaaacggcg ttgcgccgag catacaggtg
     5701 caacgctgct gctcaagcag cgcgagacac gcatcaggag tgaaaatatc taacaacacg
     5761 ctgcgagcgc caattaagaa tggtgccgtt acgccatgca gaaagcccgt tgcgtggccg
     5821 agtggcgcag gcatcataaa gacatcctgc caggtcagat tcagccgcgc gcaataagcc
     5881 cgctcactgg cgagaatatt gttatgcgtt agcatcacgc cctttggcag accctcggtt
     5941 ccggaggtaa acagcaccgc ggctaattca tcgccgtgga ccgttatcgc cgtggtcagt
     6001 ggggtattgt cggcgataat ctgacttaat gagagggaag aggtggcggg agccagtttg
     6061 tccacgccga caatttgttg tagttgtgga agctgatttt gcagcggcag gattaaatct
     6121 accggacgcg tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg acacttatta
     6181 agcacccata ccagttctgc ttcccgccag gaaggcaaca gcggtacgga aaccgcaccg
     6241 attttcaggc aggcaagata gataacggta aattcacacc agcctggcag ttgaaatgcg
     6301 atgcgatcgc ctgattcaat acccttcgct aacatccagt ttgccagaca gctcgcggcg
     6361 tgatcgagtg cgctataggt gtacgatgct ccatgattat cgaccacggc aattttgtct
     6421 ggcatcgcac gagcggtctg ctgccagtaa tcggccagcg aagcatcgcc ccatagccct
     6481 tgctgacgat acgccgcacg acgttgttcg ttaaacgtta atgtcacttt cattttggac
     6541 tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg gatgcatgat tagccgtagc
     6601 gaaaatcaat accgaaggtg cctgcgggat attgccactg ttcgagaata gtgttaccgc
     6661 acagcacccg acaggtgccg cactccagac aaccggcaga atcaaaatga atgttgccgg
     6721 catcatcctg cttataaagt ccggcagggc aggctttcat taatttttga aattctttga
     6781 tatcgggatt tgccgccaaa atgatatgcg ggtggccctc atcaacatgg aatttattga
     6841 cgcctaattt gatgtcgacg ttaaccgtag cgttctggct catagcgcgg ttgctccctt
     6901 aatgccatct ttcagcagat tgatcagccc gattttcttc gcgtgtccca tgatcatttt
     6961 gcgtaccggc tggtttggtt tgccgtcaat ggtgaacatc tcgttcatga tgtcggcgac
     7021 catgcgcgga tactgactaa acaggcgcgg attttccatc agcgccggga tcttgcgaaa
     7081 atgctgcata tcgcgcataa cacaactttg ttccagctca cgtttgtatt gcgcaagact
     7141 gctggcggag aaatccgcgc gttctttggc ggcgatcacc gttgtggcgg cagcctgagc
     7201 cgatgcaatg gctaaatcca tgccgcggac cgtaaaaccc aaattcaggc aaaagcctgc
     7261 ggcgtcacca acgatcatca cgccctcgtt aaccagttgc ggcaccattg ccagaccgcc
     7321 ttccggcacc atatgcgcgg aatattcaag cagtttgccg ccgctaatca gcgggcgaat
     7381 ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg cttttttgcg catgggcgat
     7441 atcacccaat ccacaaacca gccccaacga tatggaatcc ttattggtat agaggaatcc
     7501 gccacccatc aggccgtcag aaggggaacc ggcaaacagc caggcggcac cttcctcgcc
     7561 cgtaatatta aagcgatcgt tgatctgttc tggtgtgagg ccaataacct ctttaacacc
     7621 aacagcgtaa tgatgcggat cggaagcggg aaccattccc agcgagcggc caagcatcga
     7681 gttaacgcca tcggctaaaa tcaccacatt ggcttcgaga atatcatccc cggcctggac
     7741 tccagtgacc ttgtttcctt cacgaaccaa cgcatcgacg cgaactcccg ggataaactg
     7801 tgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc agacgattac gcaatacggt
     7861 ataagatgcg tgttgcggaa catctggttg ctcgcggtga aaatcgaggg taacggcgct
     7921 ctcttcggtt aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg gcgctgatgc
     7981 tgcaaagcct ggaatgattg cttcaagtgt gtgggcataa agacgcccgc cggtcatgtt
     8041 tttacatccg gcactgtcgc cgcgttctat caccaggaca tccagcccgg ctcgcgccat
     8101 gaccagtgcg gcaacgctac cagcaacgcc cgcaccgacc acaatggcat caaatttgtc
     8161 atccgacatg cgttatgctc ccttcccgct gcgtttacag cgggagatgt ggttgaaagg
     8221 ggaattagcg gctcaactgg ctaatcaggg cagggacgac tttgtagata tcgcccacca
     8281 gaccgtagtc ggcatagttg aagattggcg catttttatc tttattgatg gcgacaatca
     8341 cttttgcgcc gttgccgcca accatatgtt ggatctgccc ggagatcccc agcgtcaggt
     8401 agagatcgga tttcagcaac acgccggaga caccgatata acgttcacgc tccatccagt
     8461 tctcgccttc ggcaattgga cgtgaacagc cgacttcagc attcagcacc gccgccagtt
     8521 cgtggaccat tttcaggtca tcctgcgctg ccagaccacg accgacgcca accacacgtt
     8581 ttgctttgct caggtccacg ctgcttgcgg ctttcgcacg gcgttcgcga cagagaattt
     8641 catgacgcgg agcaacgtaa ggtaccgttt ctgtcgggca ctgatgagag gtatcactgg
     8701 tgcacggttc ctgaacaccg ggtgcaaggg taatgatcgc cagcgagctg ttgatctttt
     8761 cctgagcgaa cgctaacccg ccatacatcc ggtgttcggc gcaaatgtga ccatcgacaa
     8821 tatccaccgc cgtggcatcg ttcaccagcg ccgcattcag ttgcacactt aaccgtgctg
     8881 ccagcgcttt accacgtttc gtcgcggcca gtagcaacat agcggggtgt ttatctttca
     8941 gcagagcagc aatgctttcg gcgtaatttt cagtgcgttg cagcgcgtcg ttttgctcaa
     9001 gaacataaat acattttgga ccataaggaa taactgcctg cgcctggtcg gtattttgta
     9061 caatggcata cacctgttgg ccccattgct gagcgccgcc aaacagttca gcataacgtt
     9121 caggattatc gctaaagacc cagacgctgt ttaattgact catcgtttct tctccgtagg
     9181 cttcagttca gggctttctt cagatgttcc gccagctcgg caatggcctc cggcgaatcg
     9241 ttatcgagaa tgatgtgctt acgttctgtt tgcggcggta cgcgaatgcc ggtcagttca
     9301 gcaagtggtg cgctctggct ccagccaata tcacttgcct gccactgatt taccggtttt
     9361 ttacccgcgc cgagaatggc tttcatcgaa ggaatgcgtg gcacgttaat atcggaggtg
     9421 acgcagagca cggctggaac agagagttca ataacttcaa cgtcatcttc aagcgtgcgt
     9481 tcaatcacca gtgtattgcc ctgacgctga atagcactca ctgcattaat caccggaagt
     9541 tgcagaattt ctccgaccag caagccaacc tgctgggcat aaaggtcgcc ggaaccttca
     9601 ccaaagatca gtaaatcgaa gccgatcttt tcaactgctg ccgccagcgc ttttgcggta
     9661 tcgagaggca gtgcatgctc aagttgtgca tcctgcacca tatacaggct gtgcggcccg
     9721 cgggatagca cgtctttgcg cactttcgag ttctgcaaca atgagccgcc aacggtcagc
     9781 gcggctatct catcgtcatc tgttgcaagc tggcttgcag cttcaatggc attgagatcg
     9841 aactggctga ttttggcgtc ggcattgtcg aaattcaggg tgtattctgg agtgacaaca
     9901 atgtcctgtt cttcaggcac cagcttaaag caggttatta ttttcatgat ttctcctgtg
     9961 agtcgcatac ggaaccatct ccgatggaag gtaaaaatgg cctgaaaata gtgtgcgata
    10021 gcggtgcggg gttttcgacg gagcaagctg ttttccttgc caacaatctt gcaaaaaaaa
    10081 tgcgatcgct aaaacaaaag tgcaatttgc ggcaatatag ttattccatg accgtacaat
    10141 cttgcattca gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt ataaccgcaa
    10201 tacacgccgt attttatccg gcatatattg tttttgcttg ctcatggaat agggaggttg
    10261 tcatgtatca acgctgtttt gataatgcca gtgaaacgct gtttgtcgct ggtaaaacgc
    10321 cacggctttc acgttttgca tttagcgatg atccaaaatg ggagtctgga catcacgttc
    10381 atgacaatga aaccgagctg atttacgtca agaaaggggt ggcaaggttt accatcgatt
    10441 cttcgttata tgtcgcgcat gcagatgata tagtggtgat agaacgcggc aggctgcatg
    10501 cggtggcctc tgacgttaac gatccggcaa cgacgtgtac ctgtgcgctg tacggctttc
    10561 agtttcaggg ggctgaggaa aatcatctac tgcaaccgca ttcttgtccg gtaattgccg
    10621 cggggcaggg aaaagaagtc attaaaacct tatttaatga gctaagtgtg attttgccgc
    10681 aaagtaaaaa tagccaaaca tcttcgttat gggatgcatt tgcctataca ttagcaattc
    10741 tttactacga aaact
//
LOCUS       DAZFFG010000139        10672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFFG010000139 DAZFFG010000000
VERSION     DAZFFG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.139"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            35..946
                     /gene="hlyE"
                     /locus_tag="ACHJ7J_004222"
     CDS             35..946
                     /gene="hlyE"
                     /locus_tag="ACHJ7J_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO5002385.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1153..1599)
                     /gene="ycgN"
                     /locus_tag="ACHJ7J_004223"
     CDS             complement(1153..1599)
                     /gene="ycgN"
                     /locus_tag="ACHJ7J_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO5002386.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1691..2350)
                     /gene="ycgM"
                     /locus_tag="ACHJ7J_004224"
     CDS             complement(1691..2350)
                     /gene="ycgM"
                     /locus_tag="ACHJ7J_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5002387.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2422..2715)
                     /locus_tag="ACHJ7J_004225"
     CDS             complement(2422..2715)
                     /locus_tag="ACHJ7J_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO5002388.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2957..3358
                     /gene="pliG"
                     /locus_tag="ACHJ7J_004226"
     CDS             2957..3358
                     /gene="pliG"
                     /locus_tag="ACHJ7J_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO5002389.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3478..3846)
                     /gene="ycgJ"
                     /locus_tag="ACHJ7J_004227"
     CDS             complement(3478..3846)
                     /gene="ycgJ"
                     /locus_tag="ACHJ7J_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO5002390.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4366..5061
                     /gene="minC"
                     /locus_tag="ACHJ7J_004228"
     CDS             4366..5061
                     /gene="minC"
                     /locus_tag="ACHJ7J_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO5002391.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5085..5897
                     /gene="minD"
                     /locus_tag="ACHJ7J_004229"
     CDS             5085..5897
                     /gene="minD"
                     /locus_tag="ACHJ7J_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO5002392.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5901..6167
                     /gene="minE"
                     /locus_tag="ACHJ7J_004230"
     CDS             5901..6167
                     /gene="minE"
                     /locus_tag="ACHJ7J_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO5002393.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6539..6882)
                     /locus_tag="ACHJ7J_004231"
                     /pseudo
     CDS             complement(6539..6882)
                     /locus_tag="ACHJ7J_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6917..7036)
                     /locus_tag="ACHJ7J_004232"
     CDS             complement(6917..7036)
                     /locus_tag="ACHJ7J_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5002394.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6997..7182)
                     /gene="ymgJ"
                     /locus_tag="ACHJ7J_004233"
     CDS             complement(6997..7182)
                     /gene="ymgJ"
                     /locus_tag="ACHJ7J_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO5002395.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            7283..7456
                     /gene="ymgI"
                     /locus_tag="ACHJ7J_004234"
     CDS             7283..7456
                     /gene="ymgI"
                     /locus_tag="ACHJ7J_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO5002396.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7458..7802
                     /gene="ymgG"
                     /locus_tag="ACHJ7J_004235"
     CDS             7458..7802
                     /gene="ymgG"
                     /locus_tag="ACHJ7J_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO5002397.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7806..8141
                     /gene="ymgD"
                     /locus_tag="ACHJ7J_004236"
     CDS             7806..8141
                     /gene="ymgD"
                     /locus_tag="ACHJ7J_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO5002398.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8197..>10672)
                     /gene="ycgH"
                     /locus_tag="ACHJ7J_004237"
     CDS             complement(8197..>10672)
                     /gene="ycgH"
                     /locus_tag="ACHJ7J_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YcgH"
                     /protein_id="HFO5002399.1"
                     /translation="HAVKVDDGVTRYINNASVMTSGDGSYGISVDSQNKVLYISDSDI
                     KTSGSVSNKENNKDVNGGITASAVVSEFGGTIVMNGDNLVETGGAYSAGLLSQVNDSG
                     NVENNTRLETTDKTIIVTSGENAVGVLACSSPGESRTCVDAVDDEVSDSNSYEVISRA
                     DLKMNGGSITTNGINSYGAYANGEKAYINLDYVALETLADGSYAVAIRQGNIDIKNSS
                     ITTTGTKAPIAKIYNGGELFFSNVTAVSEQDKGISIDASNIDSQAKIALLSVELSSAL
                     DSIDVNKTTTDVSILNRSIITPGNNVLVNNTGGGLNIISSDSTLNGATKLVSGTTTLK
                     LSENTIWNMKDDSVVTHLTNSDSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIRT
                     VLGDDKSATDRLIVEGNTSGSTTVYVKNAGGSGAATLNGIELITVNGDESPADAFRQG
                     DARIAAGAFEYQLKQQGKNWYLTSYQSVNEEDNSSEGNSESTETPTPVLRPEAGSYVA
                     NLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSH
                     RYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYSAGLYA
                     TWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSF
                     GLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEK
                     NSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLNPAASV
                     WGNVGVQLGDNGYNDTSVMVGLKYKF"
BASE COUNT         2995 a         2500 c         2313 g         2864 t
ORIGIN      
        1 attacaattt atatatttaa agaggcgaat gattatgact gaaatcgttg cagataaaac
       61 ggtagaggta gttaaaaacg caatcgaaac cgcagatgga gcattggatc tttataataa
      121 atatctcgat caggtcatcc cctggcagac ctttgatgaa accataaaag agttaagtcg
      181 ctttaaacag gagtattcac aggcagcctc cgttttagtt ggcgatatta aaaccttact
      241 tatggatagc caggataagt attttgaagc aacccaaaca gtgtatgaat ggtgtggtgt
      301 tgcgacgcaa ttgctcgcag cgtatatttt gctatttgat gagtacaatg agaagaaagc
      361 atccgcccag aaagacattc tcattaaggt actggatgac ggcatcacga agctgaatga
      421 agcgcaaaaa tccctgctgg taagctcaca aagtttcaac aacgcttccg ggaaactgct
      481 ggcgttagat agccagttaa ccaatgattt ttcagaaaaa agcagctatt tccagtcaca
      541 ggtagataaa atcaggaagg aagcgtatgc cggtgccgca gccggtgtcg tcgccggtcc
      601 atttggatta atcatttcct attctattgc cgcgggcgta gttgaaggga aactgattcc
      661 agaattgaag aacaagttaa aatctgtgca gagtttcttt accaccctgt ctaacacggt
      721 taaacaagcg aataaagata tcgatgccgc caaattgaaa ttaaccaccg aaatagccgc
      781 catcggggag ataaaaacgg aaactgaaac aaccagattc tatgttgatt atgatgattt
      841 aatgctttct ttgctaaaag aagcggcaaa aaaaatgatt aacacctgta atgagtatca
      901 gaaaagacac ggtaaaaaga cactctttga ggtacctgaa gtctgatcag cgattattct
      961 ctccatgtac tcaaggtata aagtttatca catttaaaaa tacagacgtt ttttaaagat
     1021 tgagagttac ctgttgataa taaggtatcg ggtaactctc attatgacag agcgctttca
     1081 aaaacagggc tacgaaatgg cgagcatcca gacgtatatt aagaaagccc ccatcaacat
     1141 ctattaaatt tatcactgcg cccagtcagg tttatttaag atatgatcct gccagtcaat
     1201 cacttctgat tctttcactg cgatatgacg cacagagata cgttcaccat gcattgccgc
     1261 tttcgaacca gtaagcagcg gatgccacgc aggtaaatct ttaccttccg ccagcaaacg
     1321 ataagcgcag gtcattggca gccattcgaa tgttggcaga ttttcacggg ttaatttaat
     1381 gcagtcgggt tcaaactcga aacgacgttc gtagttccga cattgacagg ttttaatatt
     1441 gagctggcga caggcgacgt tagtgaagta gatttcgtcg gtgtcttcat ccatcagttt
     1501 atgcaggcaa cactgaccgc aaccatcaca caacgactcc cattccgcat cgctcatttc
     1561 gtccagggtt ttactttgcc agaaaggtac atcgctcatc aggtgttccg ccattacgtt
     1621 aaaacgcacc ttataaccag tctggcacag cgatgcaagt tttgccgccg ctttcaggcg
     1681 gcaaaaagta ttacaaaacg cgagttgtca aagaatgccc atcgaaagtg actgtcagct
     1741 catcaccgct ttgcaacggg ccgacgccat caggcgtgcc tgtcagcaca acgtcaccgg
     1801 ccttgagggt aaaaaacttg ctcatataag cgatcagcgg aacgatttta tggatcatgt
     1861 ccgcagtcgt accttgctgg cgttgttcgc cgtttacgct caggcccagc gttgtatttt
     1921 gcggatcgcc ggtgaattcc gccgcgggaa taaacccgga aagcggacaa gagttatcaa
     1981 acgctttagc cttttcccac ggctgcccgg ctttcttcat ttttccctga acatcacgca
     2041 acgtcagatc gagcgccacg ccgtaaccgg caatggcttt gcggacatgc tcttccgtag
     2101 cctgacgcag cgtcgcgcca atcaacaccg ccagttcgac ttcatgatga actgaaccga
     2161 aatcggatgg gatcgccaat ggctgccgca gatcgcacag tgccgtttct ggtttaataa
     2221 acagcactgg ctcttcgggc actgcgctgc ccatctcttt aatatgtttg gcatagttac
     2281 tgccaacaca aactacttta ctcaccggat aatccagcag cgcaccttgc cagttgtgat
     2341 gttgatacat aacgttcccc tcaccttatc gtggatatga aaacccgcgc cgacaaagat
     2401 aatgccaccg ccggatatcg gttatttgtt agtgtcgtct gttttctgcc ccatgacgga
     2461 aagatgttgc ttcagcaaat cttcgggtgg tggcggtaac tgcaaatagt aaccttgctc
     2521 ggttaatgcc tgtttaacct tttcaatatc ggcattgacc agtttcttcc gcccatccag
     2581 cggcagaatc atcgctaact gaggctgacc aaaacctttc atcagttcct caggaacacg
     2641 cgaaaaatcg tctttttttt cgacatataa ataggtctgg tcacgcttgc tgcttcgata
     2701 aatcacacaa aacatacttt tactctttaa gatgccaggt tttggcataa gctaacatca
     2761 atgaaacccg cgccacaagc cagccagctg tagatttcta cggtttgttc agtccatacg
     2821 cccttaataa gtaatccccc cggcaaaaat gccgcgacgg gattttaccg aacgcaggaa
     2881 agccactgga attctcccgt aatggattgt tatattataa cagttcatcg tactcattct
     2941 gaacaggaga ctaccaatga aaatcaagag catcagtaag gctgtattgc tgcttgcact
     3001 gctaacctcc acgagctttg ctgcgggtaa gaatgtcaat gttgagttca gaaaaggcca
     3061 tagcagcgct cagtactccg gcgaaataaa gggatacgat tacgatacat ataccttcta
     3121 tgccaaaaaa ggtcagaaag tgcatgtgag tatctctaat gaaggtgctg atacttacct
     3181 cttcgggcca ggaatcgatg attctgttga tttatccaga tattccccag aactcgatag
     3241 tcacggacaa tactcactcc ctgcttctgg caaatatgag ctgcgagtgc tgcaaacccg
     3301 aaacgacgcc cgtaaaaaca aaactaaaaa atataacgtc gatattcaaa ttaagtaaat
     3361 gtcaaccgac ggggagtctc cccgtcataa acgttgacgc cccactcctc tctgtcgctc
     3421 aagaggttaa tgacgaatag cagacaatgc cgaatagaac ataatgtaaa caaggcatca
     3481 ctcacgacac ataaataaca tctttgtggt ggtttgattg atttttccac tccgcttacc
     3541 atctacacca aaataacgat catcacggca taatttttct ttaacatcgc aaaatacacc
     3601 gttagcgaat gtaaattcgc tgggatcgta gccttgtaat gattgtaagt tttcagcggc
     3661 tttttcgctg agatatttcc ttgttaactc tggggaaatg ccatctgagt tcacacaaat
     3721 atacgggtcg caaacaacgc cgcacgccgg agattgaagt ccgccagcgt catgtccaga
     3781 cgccattgat gagaacatca tccccacacc tgacaatccg atataaaaaa tacgcatcat
     3841 attcatagca catcctgata atccaaccgt acaacactta tgctcatttc atcatcaatc
     3901 cgtaatcaaa accgctgacc tggacgagtt acgatgacct gacagaagta tcagacgtct
     3961 tctgcggttt ctatgataga gcaattctgt ggcggatatt aagataacgg gtatctgttc
     4021 tttaatgatg ccgcaaaaaa tggaaagtta agaaagcctg gcgtggtgaa aaaatacact
     4081 tgcctcaata ctggatattt aaacaggtct ggagatgctg tcacgattag ggtgcggaaa
     4141 aaggaagcgt aaataagaag aatcaaaagt acgacggcaa tgggttgatt gacaagggta
     4201 ttttttaagc tatgaatcag cgccatttat cacagaatag acttttactc tgaataaatg
     4261 ggagggtgac ttgcctcaat ataatccaga ctataacatg ccttatagtc ttcggaacat
     4321 catcgcgcgc tggcgatgat taatagctaa ttgagtaagg ccaggatgtc aaacacgcca
     4381 atcgagctta aaggcagtag cttcacttta tctgtggttc atctgcatga ggcagaacct
     4441 aaggttatcc atcaggcgct ggaagacaaa atcgctcagg cccccgcatt tttaaaacat
     4501 gcccccgttg tactcaacgt cagtgcactg gaagacccgg taaactggtc agcgatgcat
     4561 aaggcggttt cggcaaccgg tttgcgggtt attggcgtaa gtggctgcaa agatgcgcaa
     4621 cttaaagccg aaattgaaaa aatggggctg cctatcctga cggaaggaaa ggaaaaagcg
     4681 ccacgtccag ctcccgcacc gcaggctcca gcgcaaaata caacgccggt cacaaaaacg
     4741 cgtttaatag ataccccggt gcgttccggt cagcgtattt atgctccaca atgtgatctg
     4801 attgttacaa gccacgttag cgctggggcc gaattaattg ccgatggtaa cattcatgtc
     4861 tatggcatga tgcgcggtcg tgcgctggca ggggcaagtg gtgaccggga aacgcaaata
     4921 ttttgtacga acctgatggc ggaactggtg tccatcgcag gtgaatactg gctgagtgat
     4981 caaatcccag cagaatttta tggcaaagcg gcgcgactgc agttagtcga aaacgctttg
     5041 accgttcaac cgttaaattg atcccttttt aacaaggaat ttctatggca cgcattattg
     5101 ttgttacttc gggcaaaggg ggtgttggta agacaacctc cagcgcggcc atcgccactg
     5161 gtttggccca gaagggaaag aaaactgtcg tgatagattt tgatatcggc ctgcgtaatc
     5221 tcgacctgat tatgggttgc gaacgccggg tcgtttacga tttcgtcaac gtcattcagg
     5281 gcgatgcaac gctaaatcag gcgttaatta aagataagcg tactgaaaat ctctatattc
     5341 tgccggcatc gcaaacacgc gataaagatg ccctcacccg tgaaggggtc gctaaagttc
     5401 ttgatgatct gaaagcgatg gattttgaat ttatcgtttg tgactccccg gcagggattg
     5461 aaaccggtgc gttaatggca ctctattttg cagacgaagc cattattacc accaacccgg
     5521 aagtctcctc agtacgcgac tctgaccgta ttttaggcat tctggcgtca aaatcacgcc
     5581 gcgcagaaaa tggcgaagag cctattaaag agcacctgct gttaacgcgc tataacccag
     5641 gccgcgtaag cagaggtgac atgctgagca tggaagatgt gctggagatc ctgcgcatca
     5701 aactcgtcgg cgtgatccca gaggatcaat cagtattgcg cgcctctaac cagggtgaac
     5761 cggtcattct cgacattaac gccgatgcgg gtaaagccta cgcagatacc gtagaacgtc
     5821 tgttgggaga agaacgtcct ttccgcttca ttgaagaaga gaagaaaggc ttcctcaaac
     5881 gcttgttcgg aggataagtt atggcattac tcgatttctt tctctcgcgg aagaaaaaca
     5941 cagccaacat tgcaaaagaa cggctgcaga ttattgttgc tgaacgccgt cgcagcgatg
     6001 cagaaccgca ttatctgccg cagttgcgta aagatattct tgaggtcatt tgtaaatacg
     6061 tacaaattga tcctgagatg gtaaccgtac agcttgagca aaaagatggc gatatttcta
     6121 ttcttgagct gaacgtgacc ttgccggaag cagaagagct gaaataggcc cgctgtaaaa
     6181 gcgcatttat cttcaaggca gagtcatctc tgccttgagt ttttcatccc tctcatcgac
     6241 gttgtggtaa agcggcgagt attcttgctg atactcctca ttgctatttc cacctccccc
     6301 tttacacctt aaggctgttc ccacgcacag aacaaaatgt ttatgcttgg ttgagtaaat
     6361 aaccttattg ttagtgtgga tatactggga gatgataacc ccgcaatttc attattcatt
     6421 gttctcccat aggatgaggt ttcccgccgt aactggcggg ctttttttgc cccagaattt
     6481 tctctttcaa catcctgtaa acgaaaactg cgccgaagcg cagtttaatc gttggtggtc
     6541 agaacgacca gttcacacca gccaccgcgt tccacgggga ttccacaccg gcaccgttgc
     6601 tataccccac cccgagatgc ccgcttaacg tactgctgaa tgaggcttta atacctgcct
     6661 ggtatattcc acgtctgccc gacaaatcat tgacaaaatt accgtcacta ttcactttca
     6721 cccgattatc atcgacaaat tctttgcgca cagccgcctt cagccagggt tcatttccat
     6781 accgttcccc aggcgcatgt tgtaactcag cgttgcaccc agttcacgat atatactgcg
     6841 ggtatcgact gatttcgatt ccatgccatt ggataaatga tagtagcgtc taagtaacct
     6901 aagctcggct gcaacatcag cccgcaggca ccagaccagg ggattcatcc tgaagagaca
     6961 gcgcaggtgt attgtgttcg ccgctcacca aagacatcat gatgaaatga tgatatcccg
     7021 cataagaaag aggcattttt taaacgcagt gcgctgaagt gtagttggat aaaaaatcac
     7081 tcttttcaga taatactgtc gtacgcttat ctttcgacaa tgaggccgtc ggcaaaataa
     7141 aatgactaat gtaatcgttg ctgatgaata tcttctgctc acataaaaat cacacaataa
     7201 tattgataaa tcagattatc ttacctttac aacattgcgt taacagtcaa tgacacttct
     7261 tgaataaaaa tattgtcaat caatgagtta ttcctcattt aagattattc tcattcacgt
     7321 aaaaaacata attcctatca tcacagccac gctcattttg aattatctga ataatagcga
     7381 gcgttcttta gttaagcaga tcttaattga agacgaaatt attgtgtgtg caacttactt
     7441 aataccggac atctaagttg aagaaaaaaa tacttgcctt cgggcttatc tcagcattgt
     7501 tctgctccac gccagcgatg gcggatatga accgcaccac gaaaggtgca ttgctaggcg
     7561 caggcgttgg attacttact ggcaatggtg ttaatggcgt actgaaaggt gcagctgttg
     7621 gcgctggtgt tggtgcagta acagaaaaag gccgcgacgg taaaaatgca cgtaaaggtg
     7681 caaaagtggg tgccgccgtt ggtgctgtca ctggcgttct gaccggcaat ggcctcgaag
     7741 gggcgattaa aggtgccgtg attggcggta cgggtggtgc aattctgggt aaaatgaaat
     7801 aaggaatgat gatgaaaaaa tttgctttac tggcgggtct gtttgttttc gcaccaatga
     7861 cctgggcgca ggactacaat attaaaaacg gtttgccgtc agaaacctat attacttgcg
     7921 cagaagccaa cgaaatggca aaaactgaca gcgcacaggt tgcagaaatt gttgcggtaa
     7981 tgggtaatgc cagcgttgcc agccgtgatt taaaaattga gcaatctccg gaactgagcg
     8041 ccaaagtggt agaaaaactg aatcaggttt gcgctaaaga cccgcaaatg ctgttgatca
     8101 cggcaattga tgacaccatg cgtgctatcg gcaaaaaata atatttaata tgttaccgcg
     8161 tcgatgaatt cgacgcggta ttttagctaa cggggatcag aacttatatt tcaggcccac
     8221 catcactgag gtgtcattgt agccattatc acccagctgc acgcccacat taccccaaac
     8281 actggccgcc ggattcagat tgccatttac gccaagttta atttccccca gattacgcgc
     8341 cccgtcttgt tcgactttca caccgttcat tgagacagca aaatctttac tgttatggat
     8401 ccagttcatt tcaacaaaag gttcaaagtc gtcaccgtgc ggaccgctgt ttttctcctg
     8461 agtacgaata aaggtgcgga aaccaagtcg ggtctggatg ttgttatttg cgtcattctc
     8521 aatacgtgag ccattggctt cggtgtgcgc attatgatcg acgcccatcc agatagcctg
     8581 tgcctgtggc tggaagatcc acgtatattt cgccgcttcc agaccaaagg atttattcag
     8641 ggcaaagccg taacccgctt ccagcgagac ggttgcgcct ttcgcgctat aggattcata
     8701 ggctaattca tcccctttca ccgagttttt aaaccagcta tattgcgccc aggcgtcaat
     8761 gtatgcgcct tttttactga tgtcatcggc aaaccaggtg gcatacagcc ctgcgctata
     8821 gcctctgaca ctccctttcg aacgataatc cgacacgctg gaatgagttg agttgtagtc
     8881 gcgggcataa ccagccatca ctcccaaacg ccaactgtca ctgtcggtaa aaccaccggt
     8941 taacagatcg gcccctaact gcgagacgta gcgatgcgag gtcgttctca actgtccgtt
     9001 gctgtcacgc caggcattat gcccgccaat ttgacgtagc caaagtcggc ttgaacgctc
     9061 ctgttcagtt acaggatcga tgtaacgcgt ttcacctgca cgatcgttca gacgcataac
     9121 aaaaagagtg ttagcggctg ccaggttggc gacgtaactt ccagcttcgg ggcgcaggac
     9181 gggggtaggc gtttctgttg attctgaatt tccctctgag ctgttatcct cctcattcac
     9241 tgactgataa cttgtcaaat accagttttt gccttgttgt tttagttgat attcgaaagc
     9301 tccagctgca atccttgcat caccttgtct gaaggcatct gctggagatt catcgccatt
     9361 cacagttata agttcgatcc cgtttagcgt ggccgcgccg cttcctccag catttttcac
     9421 atagacggta gttgaacccg aagtattacc ctcaacaata agtctgtccg tagccgattt
     9481 atcatcacct aatacggtgc ggatattaag ctgaccatta ttaccgacat aattaccttt
     9541 tacagttaat gtttttcctt gggtaaatgt ttgaccatca tcatacgaaa gattgataat
     9601 actgtctgaa ttagtcagat gggtaacaac ggagtcatct ttcatattcc agattgtgtt
     9661 ttctgaaagc ttcagcgtgg ttgtgccgct aacgagttta gtcgctccat ttagagtaga
     9721 gtcggacgaa attatgttta agccacctcc agtattatta accagaacat tattaccagg
     9781 tgtgataata cttcgattaa ggatacttac atccgttgta gttttgttaa catcaatact
     9841 atccaaagca cttgaaagtt caacacttaa tagtgctatt ttggcttgag aatcgatatt
     9901 tgatgcatca attgatattc ctttatcttg ttctgatacc gcggtgacat tggaaaaaaa
     9961 taactctcca ccattgtata tttttgcaat gggggcttta gtgccggttg ttgtaataga
    10021 actatttttt atatcaatgt taccttgtcg aatagcaact gcataacttc catcagccaa
    10081 agtttcaagt gccacataat ctaaattaat atatgctttt tccccattag cataagcacc
    10141 atagctatta atgccattag ttgttatgga accaccattc atttttaaat cagcacggct
    10201 aataacttcg taactgttag aatcactaac ttcatcatct acagcatcga cacatgttcg
    10261 agactctcca ggacttgaac atgcaagaac acctactgca ttttccccag aggtaacaat
    10321 gatcgtttta tctgtggttt caagacgagt gttattttcc acatttccag aatcattaac
    10381 ctgacttaaa agtcccgctg aatatgcccc ccccgtctcg actaaattat caccattcat
    10441 aacgatggtg ccaccaaatt cactgactac tgcgctggct gttatccctc cattaacatc
    10501 tttgttattt tctttgttag atacgcttcc agaggtctta atatcgctat cgcttatata
    10561 taatactttg ttttgactat caacagaaat accataagat ccatcaccac tagtcatcac
    10621 actggcatta tttatatatc tagttacacc gtcatccact ttcacagcat gg
//
LOCUS       DAZFFG010000140        10658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFFG010000140 DAZFFG010000000
VERSION     DAZFFG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.140"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(249..1946)
                     /gene="treA"
                     /locus_tag="ACHJ7J_004238"
     CDS             complement(249..1946)
                     /gene="treA"
                     /locus_tag="ACHJ7J_004238"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5002400.1"
                     /translation="MKSPAPSRPQKMALIPAYIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPT
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTIRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPPSESTTQPVKQKEAEPTP"
     gene            complement(2266..3684)
                     /gene="dhaM"
                     /locus_tag="ACHJ7J_004239"
     CDS             complement(2266..3684)
                     /gene="dhaM"
                     /locus_tag="ACHJ7J_004239"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO5002401.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSAVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            complement(3695..4327)
                     /gene="dhaL"
                     /locus_tag="ACHJ7J_004240"
     CDS             complement(3695..4327)
                     /gene="dhaL"
                     /locus_tag="ACHJ7J_004240"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5002402.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4338..5408)
                     /gene="dhaK"
                     /locus_tag="ACHJ7J_004241"
     CDS             complement(4338..5408)
                     /gene="dhaK"
                     /locus_tag="ACHJ7J_004241"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5002403.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5636..7555
                     /gene="dhaR"
                     /locus_tag="ACHJ7J_004242"
     CDS             5636..7555
                     /gene="dhaR"
                     /locus_tag="ACHJ7J_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO5002404.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVKQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7655..10540)
                     /locus_tag="ACHJ7J_004243"
     CDS             complement(7655..10540)
                     /locus_tag="ACHJ7J_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5002405.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGATGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDEMP
                     LYVGLYIGSTHASPGYSGGDGTARSDYMGMYASYMAHSGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGKSQLNIYVKTGAIHEFSGDTEYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2539 a         2643 c         2680 g         2796 t
ORIGIN      
        1 taaataacac tttgttccat aagaaaattg tcagggagaa tggcggagtg gcgaaaaata
       61 taaatagagg tgtaatgatt aacaaattga taaaataaaa ttaaaatctt ctctgataaa
      121 agaaaataaa ggcatagacc gtagaatggg gggcatttcg tggcgaaaaa agagcaatgc
      181 caattttatg cacataatgg cattgctcga tatacagcct cgatatacag cctcgatata
      241 cagcaaagtt aaggcgtagg ttccgcctct ttttgtttta ccggctgtgt tgttgattca
      301 ctgggtggac gcgtcgccgg aacattgtca cacggttgct ctttcgggca gatcaaatcc
      361 agcattttca gcgtcacgcc attggtccag ccaaagccat cctgtaatgg atattcgcca
      421 ccgccgcccc ccgttccggt ggtgctgaca tcatattttt ccaccagctt tttctcccgg
      481 tcataggtgt gctgaacatt ggtcaggaag tgccagctaa tgtccatcgc cacctctttt
      541 tgaccgtagt tttgtaatcc ttctgtcgcg acccactgca acggtgccca gccatttggc
      601 gcatcccatt gttgcccact tttcaccgac gtggtgttca ggccgcctgg ttgcagcaga
      661 tgtgttttcg tcgccgtcgc cattttgttg gcgcgatctt tcgctgccgc attgacgtac
      721 agcgggaaca gggcggcagc ggttaactga ttgcgcactt tatgactttt caggtcgtaa
      781 tcggcatacc agccttgttg atcgttccac aggtattttt cgatcccttt ttgacgggca
      841 tttgccagcg tttcgtactg gtttgccatc gcgttatctc cggcagcctt gctggcgcgg
      901 gcgaggattt tttccatttt aaacatcagg ctgttcagat cgaccggtac gatgctggtg
      961 gtgcgtatgg tatttaactg ctgcggattg tccatccagc gcgagctgaa atcccagcca
     1021 gacgcagcgg cagagcgcag gtcgcggtaa atttcagtgg caggtcgatt cggattgctt
     1081 ttagcggtgg caatatcttc cacccatgac tctggtcgtg gcgtatcgcg atcgtcccag
     1141 tagcggttga gaagggtacc atcctgaagt tttacaacgc gtttttcctg ttgtccggct
     1201 tgcaggtttt caacaccgtc catccagtaa gcatattctt tttgcatttg cggcaggtat
     1261 tgcttcaatg cggcatcgcc ttcatgctgc gccagtaact ctaccatcag ggcaaagaag
     1321 ggcggctgcg agcggcttaa atagtaactg cggttgccgt tgggaatatg accgtaagtg
     1381 tctatttcat gagcaaaatt ggccaccata tccgcgactt tatcccagtg accgctttcg
     1441 gcaagtccta acatggtgaa gtaactgtcc cagtaatata cctcgcgaaa acgtccgccc
     1501 ggcacgacat aaggttccgg cagcggtaac agagaatccc atttttcggt gttttcggta
     1561 gaacgcgtta ataccggcca aagtccgtca atatgttcgc gcagtgactg tccctctggc
     1621 ggaacatatt tctcgccctc tgtcggcagg gtgaaattga cgttaacgaa atggcgcaga
     1681 tcaaatccgc tctggttttg ctgcatccga taatcagcaa ggatcatcag cggatcgctg
     1741 ttcggcacgg catcggcaaa ggttttttgg tccggaaaaa gtttggcgtt ttgcacatca
     1801 ttaaacagcg gccctaataa aatatcaggc ggctgtggcg ttaccggtgt ttcttctgcc
     1861 tgcaccgata gcgcagcgaa acacaaaaag atgtaggctg gaattaacgc cattttttgc
     1921 gggcgagaag gtgcggggga tttcatcaat cattctcctt aggcgaaacc gaataaaacg
     1981 gtattcaagt atcagaaaac cttagttcag gatcgcacta gctgcatgat cgaaatctca
     2041 ttctgcgaac tgtcaggcat tttagaaaag ggaattaaag ggataaataa cggcgaaata
     2101 gagtgatcaa ataacgtctg tttattagcc agccagcggc gtttggttcg cgattagcga
     2161 agaaaaatta tcataaattg gtgaaaaata taacaggtag ttaaaaaaca ttagtgctga
     2221 gtaaattgcc ggatgacatc agaacgatgc catccgaacg gtgacttaac cctgacggtt
     2281 gaaacgttgc gttttaacgt ccagcgttag cttttcttct ggttgtatcg catacagttt
     2341 ctcaccctgc tggcaaatcc agccaatccc cagttcacgg gcgatcaggg cgctgtggga
     2401 taccggactt ccggcgctaa ggcagatacc ttttacaacc gccggatcca gttgcagtac
     2461 tgcggaggga taaatgttct ccgccagtag aatagttggc gagttaaact gcgggagttc
     2521 ttctttcgtt tgggtcaggt ggaccagggt gcgatgcaga agatcgtcca catcaatata
     2581 gcgagcttgt agatattcat catccagttg ctggtattgc tggctaagtt ctttaagaac
     2641 ttgctgccag gcatattctg ccgtgcaatg ttcgtgctga aggagttcgc ttgccgccgc
     2701 cagcagttcc ggatcatcta acagtgtatg gtgaccagaa aagattgcgg caatatcgtc
     2761 aagcccgctg gcttctgctt tcgctgttaa cgtcatcaga tctaataacg tgaagtcaat
     2821 ggcctggcgt aatcgatctt gttcctcttc cacggtcagg gttgatttgg cctgtaccgt
     2881 acataaaacc ggttgataat aaaaggcttt acccgaaaca ggcggaacgg gacgcagagt
     2941 aggtggagca acttcctccg tttcaccaaa gttatcttca gccagctgac ggaaagcgat
     3001 cagtgcctct tcagcttctg gccctttcgc aatcaggcgc agcgtatcgt tatagcgaac
     3061 ttgtagtaac gcaatctggt taatactctc cggtgtgacg catttgccgt ttttctccag
     3121 caacatatcg gcattaaatg tcgataaggt ataaaccagc cgggaggctg gacgtacatg
     3181 caggccgtta cggttcttta tgaccaccgc cagagaacgg gcttcttcat cgtacgcagg
     3241 acatgagtca gagatttcag tgtcggagga cggtaaaccc agttgttcac gtttggcttc
     3301 cagcgcatgc atggcgtcaa agataacttt gtcgatatcc gcccccgagg ccgcgctgac
     3361 cgttgctgcc agtgtacctt cgaccaacgg tgcagcacac aaacgtactt ttgcagcgat
     3421 ctcgggagcc agcaattcca gcgcagtttc agcactcaat aatgcgctac ccatatccat
     3481 catgaccagc acatggtcgg catcggcaac agattctatg gcctccatca ctttgacggc
     3541 atcggtacca atgggatttt gtggatcgtc aattcccgca gcaatggcga ttttacaatt
     3601 atcactcatt aacatctgac gggctaattc accgacacct tctcccagtc ggctgctatg
     3661 tgaaactatg accaggttta ccatcaccaa ttccttactc ttttgcggct aacgccaaca
     3721 tttgcatcat aaacatcacc gaggttgcgc cgggatcctg gtgaccaata ctgcgttcac
     3781 cgagataact ggcgcggcct ttgcgggctt gcatcgtaat cgtactttgt gcagcggatt
     3841 cggcgatgct actggcagct tcgagcgccg ccggaacaga gagattttgc tcgctggact
     3901 gacgtaacga ttccaccacc ggcacccaca catcacacat ggttttatcg ccaggttcgg
     3961 ctttcccgcg actgattacg ccgtccgcgc catcgcggaa catctgataa agctcttcca
     4021 gcgtcaggct ttgccgtgcc tgggtcgcct gtgcggcgcg tataaagaag gtaccgaata
     4081 gcggaccact ggcaccaccg acgctggaaa gcagcgtcat accggtattc ttgagaatga
     4141 aaccgatatc tttatctgcg atagcaggga gtttttccac cactttgcta aagcctcggt
     4201 tcatatttag cccgtggtca gcgtcgccaa tctcgcgatc cagtccagta agatactcgc
     4261 tctcggtgtt gaaaatatcg ccacaacgag tgagccagtt aacaatttga gttctgctca
     4321 gtgacattgc tttctcctta tttaccccag ttaagggccg gggtgtggac cggggcgtcc
     4381 cagagtgcca gcgtttcgtc atcaactttc agtaaggtga ttgagaaacc ggtcatatcc
     4441 agtgaggtgc agtacgcgcc aattaaatta cgctcgatag tcaatcccgc ttgctggcaa
     4501 cgtgtggtca ggcggttata gacgccgtac agctcagaaa gtggagttgc gccaagatta
     4561 ttaaccagcg caatcaccct gtcgccagac tggagcggtt gtttggtttg tggttcttcc
     4621 tgccagctgc cttgttgata atcccagaaa cgcaaagtgc gatggtatga gccattttcc
     4681 agcagggtgt cgaacatttc atcgacggtt tgatcaaggg aagagaaggg gcggcggtca
     4741 atacccggct caccatgaat gccgacgcca aactccatct cattgtccgc cagggtaaaa
     4801 gaaggtttgc ccgcggcagg aacggtacag gcaccgagag cgatacctat tgagtggcct
     4861 tgattattca acttacgccc cagttccgca caggcgtcca gtgagtcgcc acgctccgcc
     4921 gctgcgccta cgagtttttc aattaatacg gtgttggcaa cgccgcgccg cccggcggta
     4981 taaagactgt cttttactgc aacgtcgtca tcaatgacca cagtggtcac ttttacgccg
     5041 ctatcgtgca gtaactcggt cgctgtttcg aagttaagaa tatcgccggt gtaatttttg
     5101 ataatcaaca gtacaccttc gccgccatca acttgcatgg cgcattcaaa gattttatcg
     5161 ggcgtcggtg aggtgaaaat ttcgcccgga caggcccccg aaagcatccc ctgaccgata
     5221 taaccacagt gcatcggctc gtgtccgctg ccgccacccg acagcagggc gacttttcct
     5281 gcaacagggg catcagctcg ggtgacatac accggatcct gatgcagtgt cagcgatgga
     5341 tgcgctttcg ccagtcctgc cagttgttcg tccagtacgt cttgcacatc attgatcaat
     5401 tttttcatta ttttgctcca gcaattatgg tagggcatgg atgatgttca acgacacggc
     5461 gaccactgac tgccgatgaa tccattgtgc atcaggcaag ggaaaagaaa attagcccaa
     5521 attatgtttc atagtgaaac atatgcttta atgaatgttc catattgaaa cttttacgtg
     5581 tattaatact taaaattgcg agccggaaca ccttttgtca taagggatgc gggatatgag
     5641 tggcgctttt aacaacgatg gtcggggcat atctccctta attgcaacct cctgggagcg
     5701 atgcaataag ctgatgaaac gggagacatg gaacgtacca catcaggccc agggcgtgac
     5761 atttgcttct atttatcggc gtaagaaagc gatgctgacg ctcgggcagg ctgcgctgga
     5821 agatgcctgg gaatatatgg caccgcgaga gtgtgcgctg tttatcctcg atgaaaccgc
     5881 ctgcattctc agccgtaatg gcgatccgca aaccttgcag cagctaagtg cactgggatt
     5941 caatgacggc acgtattgcg ccgagggaat tattggtact tgtgcgctat cgttagcggc
     6001 tatctctggt caggccgtga aaacgatggc cgatcaacat ttcaaacagg cactctggaa
     6061 ctgggccttt tgtgcaacgc cgttgtttga cagcaagggc cgattgacgg gaacaatagc
     6121 gctggcgtgt ccggttaagc aaactaccgc agctgatttg ccgttgacgt tggcaatcgc
     6181 ccgcgaggtc ggtaatttac tgctgacgga cagtttgctc gctgaaacta accgtcattt
     6241 aaatcaactt aatgccctgt tagaaagtat ggatgatggc gtgattagct gggacgagca
     6301 gggtaatttg caatttatta atgcccaggc ggcgcgggtc ttgcgccttg acgcgacggc
     6361 aagtcaggga cgggcaatca ctgaactctt aacgttaccc gccgtattgc aacaagcaat
     6421 aaaacaggca catccgctca aacacgtaga agcaaccttt gaaagccagc accagtttat
     6481 tgatgcggtg ataaccctta aaccgataat agaaacgcag ggaaccagct ttattttgtt
     6541 gctccatcct gtggaacaga tgcggcagtt aatgaccagt caattaggaa aagtcagcca
     6601 taccttcgct catatgccac aggacgatcc gcaaacccgc cgcttgattc attttggtcg
     6661 ccaggcggcg cgcagtagct ttcctgtcct gctttgtgga gaagagggcg tgggcaaggc
     6721 actgctaagt caggcaattc ataatgaaag cgagcgtgct gcaggtcctt atatcgccgt
     6781 caattgtgag ttatatggtg atgcggcact ggcggaagaa tttattggtg gcgatcgcac
     6841 ggacaatgaa aatggccgtc tgagtcggct ggaactggcg cacggcggca cgctgtttct
     6901 tgaaaagatt gaatatctgg cggtggagtt acagtctgct ttgcttcagg ttatcaagca
     6961 gggtgttatc acgcgactgg atgcgcggcg tttaatacca attgatgtca aagtgattgc
     7021 tacaacgacc gcggacctcg caatgctggt ggaacaaaat cgttttagtc gccagctgta
     7081 ttacgcgctg catgcatttg aaattaccat cccgcctctg cgtatgcggc gtggcagcat
     7141 tccggcgctg gtgaataaca aattacgcag tcttgaaaaa cgcttctcta cgcggctgaa
     7201 aattgatgac gatgccctcg ctcgcctggt ttcctgtgca tggcctggca acgattttga
     7261 actttacagc gtcatcgaga atcttgctct gagtagtgat aatgggcgca ttcgcgtcag
     7321 tgatttgccg gaacatctgt ttaccgagca ggcgacagat gatgtcagcg ccacacgcct
     7381 ttccaccagt ctgtcatttg cggaagttga aaaagaggca attattaacg cagcccaggt
     7441 cacaggcggt cgcattcagg aaatgtcggc tttacttgga atcggccgca ctacgctgtg
     7501 gcggaaaatg aagcaacatg gcattgatgc agggcagttt aagcgccggg tatgaaagac
     7561 agaaacgatt tctgatacat cagagtgatc tatatttcat tccggctcac gctaacattt
     7621 ttcagcatcg tttaagggct tgtctatccc gcacttaaaa gctgaagcga tatcctccgt
     7681 tgacttgctt ctgatcaaag aggttaccct gcgtgtaatc cgcttcgagg tagaatgtgt
     7741 gctgtttgtt atactgtgca ctgactccca cgccgttatt ccagccatta cctttgaaac
     7801 tgtacttctc ccgggagttg ttcaacagat attctgtatc ccctgaaaac tcgtggatag
     7861 cgccagtctt cacatagata ttcagctggc ttttacctgc ggtgatgtca tatcccagta
     7921 tcatgctggc acgccccagc agcgattcgt agtgattcag atgtatattg agaccattac
     7981 tcgccttcat agccatctca ttctggtggc tgtatgtgag ctgggtttgc ggctctatat
     8041 agaacccata accagtaggg gtcaggttga acctctgccc ggcttccagg gagatgctca
     8101 atccattcgc agtgccgttg gcgttaacgc cgttgttctg actgtccagt acgtggaaac
     8161 tatttttctg gcgcgatgct tttacaacga gatcgctgta aaaaccgctg tgtgccatgt
     8221 aactggcgta cattcccata tagtctgaac gtgcggtacc gtcgcctccg ctatagcccg
     8281 gcgatgcatg tgttgagcca atatacagac cgacatacaa cggcatttca tcagagagac
     8341 gtttatcccc accaaactgg ataccgctgt aacccatgtc aaagccgctc agtttgccac
     8401 tggcgaagga gtccaggctt cccccataac tgcgcagcca gatattaccg tctttactct
     8461 gatttcgcag gtcacccatc cgttgcatca gcgtacggtt ttcaacatag ttcaataagt
     8521 aaccaacatt gagataattg ccgccagcat ctgcggtcgt ggtgggttta ggtgtgggag
     8581 tggggttagg cgtaggatcg ggttcaggcg taggatcggg gttgactatg ggaggctgag
     8641 cgggagccgg tgttggttca ggattaggag tcggttccgg aactgtcccg gaagcgtaaa
     8701 gctcccagtt agtgccattt ttacgcacat cgtacagata tcctcccaac tcaacctgag
     8761 aagacgcgct gaacgaggcc gcaccgtcag tggtttttac cactgtcaga acttcatttc
     8821 ccgttgtggc ctcgctgccc tggttgcgga tagccaatac gtgattacca gcactactcc
     8881 cgctgatatt caataaatcc cctttattat taacgccatt accctcgcca acaacatcag
     8941 cacgcataat aaaggtactg ttaccgctca ggttctctac gtttaatgtg gtaaatgtgc
     9001 cggcagttga cccgtggctg gcaaaatcga cagttgaatg gctcagcgcc agcgtgtcga
     9061 gattagaatt acttgttacg ttccagacgc tgttattcat tgcaacgtcc agtttcccgc
     9121 cattgacatt atcgctgagg gaggaacctg tccaaaccga cccagggtgc atatccagat
     9181 tgattagccc acctttggaa agaacgctac cgttgataag catacgaccc gaggcgttga
     9241 tgcggctggc ggcataacca tcgtcatgtt gcgttgcgat ggccatctgg tcgggtgtac
     9301 tcatatcaat gaccagatcg ctcgtgaggt cgatctggct gttggtcatg gcataaatcc
     9361 ccctggctcc tgtcgcgccg gtgatagcca aactgtctcc ggttattcta ccgccgctga
     9421 gcgcccacaa acccagcgcc agactgccat tacgatcaac cgtaatatcg gtattttgca
     9481 tgttgataac agccgttgcc gtctgggccg aggcaccata agaaccgccg gaaaagatgc
     9541 tgtttcgttg cgctgccgtg ccagaaaaat tgattgtcgc gtctgaacca ctggtgacga
     9601 gaccaccgcc ctgcgcggaa gaaatatggc tatctgcacc gatagtggtt gtaccaccac
     9661 gaacttcgac gccattggcc gcggctccag ttacatcaac agtgagtgca ttcgcgctaa
     9721 cctggccaaa gctccagagg ccgtgtgcat tatcgccact ggttttaatg gaactgtttg
     9781 ttccgagatc gacaacagag tttttctgta cgtttatccc catggcgctg tagccctgaa
     9841 catcgattgt caggtctgtc gccgtaaaac gcgcagcacc attggcgtta ttgccgttga
     9901 gaccaccgat ataaacacct gtacttccat cggtcttgat tttacttccg cttccaagat
     9961 cgacactggt gccatagtca ttgatggtta ggcctatacc gttcgagttt tcaatggtga
    10021 attgagtggc tgttagtgtt gagctatgcc caataatgat gccgtcatcg ttactcttaa
    10081 tggtgctgcc tgtgcctaag tcagcatggg tatagtcacc aattaaatta atgccgatgg
    10141 cagaggtttg accaacaaca tctactgtga gtcggttagc tgtcagggag ctgtttttgc
    10201 cctgaataat gattcctttt gcatatccag aggcgtcgtt gacattgacg gtgacatcat
    10261 tacccaggtt gatattgcca ggcgtgtttc ctgctggggt cattacacca tagaggtttc
    10321 ctgatggatc ggcggtatct cctgtaattc tgtcaccatc attaatagta atattggtgc
    10381 catattgact tcctgtatca gcagctatca gcagggggga aaagataatt gcattcaaac
    10441 caatagcgcc aaattttatc agttgaattg agggtgaacg gatttttaat tcagcgagac
    10501 gatcggcttt ggtattccca ttgtgttgtt tgatgcccat accactccta tatagtaccc
    10561 aggctatggg tctatggatt ttgtcatttt gtttaagtca atgaataata tcctcttatc
    10621 atcatgatat ttgtatggtg caatcatgat gagcttct
//
LOCUS       DAZFFG010000141        10613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFFG010000141 DAZFFG010000000
VERSION     DAZFFG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.141"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            1..2104
                     /locus_tag="ACHJ7J_004244"
                     /pseudo
     CDS             1..2104
                     /locus_tag="ACHJ7J_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            2441..3418
                     /gene="glaH"
                     /locus_tag="ACHJ7J_004245"
     CDS             2441..3418
                     /gene="glaH"
                     /locus_tag="ACHJ7J_004245"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO5002406.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLTPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3437..4705
                     /gene="lhgO"
                     /locus_tag="ACHJ7J_004246"
     CDS             3437..4705
                     /gene="lhgO"
                     /locus_tag="ACHJ7J_004246"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO5002407.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIERECLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4728..6176
                     /gene="gabD"
                     /locus_tag="ACHJ7J_004247"
     CDS             4728..6176
                     /gene="gabD"
                     /locus_tag="ACHJ7J_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5002408.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKDVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6190..7470
                     /gene="gabT"
                     /locus_tag="ACHJ7J_004248"
     CDS             6190..7470
                     /gene="gabT"
                     /locus_tag="ACHJ7J_004248"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO5002409.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFAEAKQ"
     gene            7708..9108
                     /gene="gabP"
                     /locus_tag="ACHJ7J_004249"
     CDS             7708..9108
                     /gene="gabP"
                     /locus_tag="ACHJ7J_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO5002410.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9129..9791
                     /gene="csiR"
                     /locus_tag="ACHJ7J_004250"
     CDS             9129..9791
                     /gene="csiR"
                     /locus_tag="ACHJ7J_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO5002411.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEARDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRQTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9792..10241)
                     /gene="kbp"
                     /locus_tag="ACHJ7J_004251"
     CDS             complement(9792..10241)
                     /gene="kbp"
                     /locus_tag="ACHJ7J_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO5002412.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10325..10483)
                     /locus_tag="ACHJ7J_004252"
     CDS             complement(10325..10483)
                     /locus_tag="ACHJ7J_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO5002413.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2629 a         2652 c         2777 g         2555 t
ORIGIN      
        1 ttatcctcag gatttaaaag acagagaaat tatatcaact tttcacgcag aaaaaatcat
       61 taatgatctg ttaaaaaacg attataaaat ttcccttttt ccagatacaa cttaccgtga
      121 actgaataaa gctgcacagc gtagtattac agcgccagac aggataggag aagggaaaac
      181 atgggtttat caacgagata caatggttga aagaggtgat aacagcggtg ttcatcagta
      241 tggtcctgct gaacatttca cccacattat atctgacaaa ccctccccaa aagataaata
      301 tgttgcatat gctattaaca ttcctgacta tgagctggca gccgatgtat ataatattaa
      361 cgtgacgtca ccttccggac agcaagaaac atttaaaata ttaatcaatc cagaacatct
      421 acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat gtgaaatcat
      481 cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct accagcaaat
      541 cagggaaaat atgtctgagt ttgcacgttg ccattatggg tatatacaaa tccctccagt
      601 gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt actggtttca
      661 cgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggagatt tgcaggattt
      721 tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg attatacctt
      781 taactttatg ggaatcgggg gtagtggtaa aaatgacctg gattatccct ctgctgatat
      841 acgagcgaag atcagtaaag atatagaagg tggtatccct ggctattggc aaggtcaggt
      901 tttgattcca tttattatcg atccagtaac aaaagaacgt aaacaaatcc atccagaaga
      961 tatacatctc actgcaaaag acttcgaagc aagtaaagat aacatctcta aggatgaatg
     1021 ggaaaacctc catgcattaa aagaaaagcg tttaaatgga atgcctaaaa caacccccaa
     1081 aagtgaccag gttattatgc tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt
     1141 acgaggttta cgttatgatg cggcaaaaca ctcaaaacat gaacaaatag aaagatcaat
     1201 aaccccaccg cttaaaaatt ataatgagcg attacacaat actaatttat ttaacccaaa
     1261 atatcaaaaa aaagccgtta tgaattacat ggaatatctg gtaacttgtc agttggatga
     1321 acaacaaatg tcatcgctgc tttatgaaag agatgattta agcgccattg atttttcatt
     1381 gctcatgaag acgataaaag ccttttcatt tggtggcgat ctccaaaccc ttgcatcaaa
     1441 accgggttcc acaatctcaa gcatcccatc agaaagacgg atattgatta acattaacca
     1501 cgatttccct aacaatggta atcttttcaa tgactttcta tttaaccatc aacaagatga
     1561 acaattagca atggcatata tggccgctct cccgttcagc aggcctttag tttactggga
     1621 tggccaagta ttaaaatcaa cgactgagat taaaaatgat gatgggtcca cgcgtgtcgg
     1681 cggtgaggcg tggcttaata aaggttgctc tacctatcag tagctctaca atgaattcca
     1741 cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat ttgcaactaa
     1801 aaacgttctg gcctttagtc gtggggattc tgtgaatatt aatcactctc ctcatgacgg
     1861 actagttata ataaataaag gaaacgaaga aatagaaggt gcctggccta acaaattgca
     1921 accaggaata tataaaaata tggggagtaa tagcgttaac attattatta ataatacacg
     1981 caaaagtatt ccccctggta aagtatttac gcttaaaggc ggaactctaa atatcaacat
     2041 tcctggccgt agcgctcttc ttttagggaa aacaggagaa ctgccgaact atctctattt
     2101 gtaatttaat gttatatctg ccccgctaaa acggggcaga taatatgttt agtttactga
     2161 cggtcatttt gcagtgaagc catttactgt tttttatcga ccagataatc tgttctctga
     2221 tggtaattcc cccctaacct gttgctttag ttattcattt cctgtctcac tttgccttaa
     2281 taccctacgt taaatgttac taatttgttg tttttgatca caataagaaa acaatatgtc
     2341 gcatttgtgc gcatttttca gaaatgtaga tatttttaga ttatggctac gaaatgagca
     2401 tcgccatgtc accctacata tcataagagg atcgcttctg atgaatgcac tgaccgccgt
     2461 acaaaacaac gctgtcgatt caggccagga ctatagcgga ttcaccctca ccccatcggc
     2521 gcaatccccg cgtctgctgg aacttacctt caccgaacag acgaccaaac agtttctcga
     2581 acaggttgcc gaatggcccg tacaggcgct ggagtacaaa tcctttctgc gttttcgggt
     2641 aggcaaaatc cttgacgatc tgtgtgcgaa tcagctgcaa ccgttgctgt taaagacgtt
     2701 gctaaaccgc gctgagggtg cgttgttgat caatgcggtg ggggtcgatg atgtgaagca
     2761 agcggatgag atggtgaagc tggcgacggc ggtggcgcat ctgattggtc gctccaattt
     2821 cgacgcgatg agcggtcagt attacgcgcg ttttgtagtg aaaaatgtcg ataactcaga
     2881 cagctatctg cgtcagccgc accgcgtaat ggagctgcac aacgacggta cctacgttga
     2941 agagatcacc gactatgtgc tgatgatgaa aatcgacgag cagaacatgc agggtggaaa
     3001 ttcgctgctg ctgcatctcg acgactggga acatctggac cactatttcc gccacccgct
     3061 ggcgcgtcgc ccgatgcgct ttgccgcgcc gccaagcaaa aacgtcagca aagatgtttt
     3121 ccatccggtg ttcgacgttg atcagcaggg tcgcccggtg atgcgctata tcgaccagtt
     3181 cgtccagccg aaagacttcg aagaaggcgt gtggttgagc gagctttccg atgccattga
     3241 aaccagcaaa ggcattcttt ctgtgcccgt tcccgttggc aaattcctgt tgattaacaa
     3301 cctgttctgg ctgcacggtc gcgaccgctt tactccgcac ccggatctgc gccgtgaact
     3361 gatgcgtcag cgtggctatt tcgcttacgc cactcaccac taccagacgc atcagtagcg
     3421 caaaggaatt gagcggatgt atgattttgt gattattggc ggtggcatca tcggcatgtc
     3481 gaccgccatg caactgattg atgtctaccc ggacgcccgc attgcgttgc tggagaaaga
     3541 gtccggcccg gcctgtcacc agacgggcca caacagcggc gtgatccatg ccggggttta
     3601 ttacacgccc ggcagtctga aggcgcagtt ttgcctggcg ggaaaccgcg ccaccaaagc
     3661 cttttgcgat caaaacggca ttcgctacga caactgcggc aagatgctgg tcgccacgtc
     3721 tgaactcgaa atggaacgga tgcgcgcctt gtgggaacgc acggccgcga acggtatcga
     3781 gcgcgagtgc ttaaacgccg atgaactgcg cgagcgcgaa ccgaatatca ccgggcttgg
     3841 cggtattttt gtgccgtcca gcggcattgt cagctaccgc gaagtcacgg cggcgatggc
     3901 aaaaattttc caggccagag gcggcgagat tatctataac gccgaagtca gcggccttag
     3961 tgagcataaa agcggcgtgg tgatacgtac ccgtcagggc ggcgactatg aagcatcgac
     4021 gctgattagc tgttccgggc tgatggctga ccggctggtg aaaatgctcg gcctcgaacc
     4081 gggctttatt atctgcccgt tccgcggcga gtatttccgc ctggcgccgg agcataacca
     4141 gattgttaac catctgattt accccattcc cgatccggca atgccgtttt tgggcgttca
     4201 tctcacccgc atgatcgacg gtagcgtcac cgtagggcca aacgcagtgc tggcgttcaa
     4261 acgcgaaggc tatcgcaagc gcgacttctc atttagcgac acgctggaga ttttaggctc
     4321 gtcggggatt cgccgggtgc tgcaaaacca tctacgctca ggactgggcg agatgaaaaa
     4381 ctcgctgtgt aaaagcggct atctgcggct ggtgcaaaag tattgtcccc ggctttcgtt
     4441 aagcgatctc cagccctggc ccgccggtgt gcgggcgcag gcggtatcgc cggacggcaa
     4501 gctgattgac gattttctgt ttgtcaccac cccgcgcacg atccacacct gcaatgcgcc
     4561 ctccccggca gcgacatcag caattcctat tggtgcgcat attgtcagca aggtacaaac
     4621 gctgttggca agccagagta accccggacg cacgctgcga gcggcacgta gtgtggatgc
     4681 cttacacgcc gcatttaatc aataatcttt gaaaacagga tgtagcgatg aaactgaacg
     4741 acagtaactt attccgccag caggcgttga ttaacgggga atggctggac gccaacaatg
     4801 gtgaagtcat cgacgtcacc aatccggcga acggcgacaa gctgggtagc gtgccgaaaa
     4861 tgggcgctga tgaaactcgc gccgctatcg acgccgccaa ccgcgccctg cccgcctggc
     4921 gtgcgctcac cgccaaagaa cgcgccaaca ttctgcgcaa ctggttcaat ttgatgatgg
     4981 agcatcagga cgatttagcg cgcctgatga ccctcgaaca gggcaaacca ctggctgaag
     5041 cgaaaggtga aatcagctac gccgcctcct ttattgagtg gtttgccgaa gaaggcaaac
     5101 gcatttatgg cgacaccatt cccggtcacc aggccgataa acgcctgatt gttatcaagc
     5161 agccgattgg cgtcaccgcg gctatcacgc catggaactt cccggcggcg atgattaccc
     5221 gtaaagccgg cccggcgctg gcggctggct gcacgatggt gctgaagccc gccagtcaga
     5281 cgccgttctc tgcgctggcg ctggcagagc tggcgatccg cgcgggcatt ccggctggcg
     5341 tgtttaacgt ggtcaccggt tcggcgggcg cggtgggtaa tgaactgacc agcaacccgc
     5401 tggtacgcaa actgtcgttt accggctcga ccgaaattgg ccgccagtta atggaacagt
     5461 gcgcgaaaga catcaaaaaa gtgtcgctgg agctgggcgg caacgcgccg tttatcgtct
     5521 ttgacgatgc cgatctcgat aaagccgtgg aaggcgcact ggcctcgaaa ttccgcaacg
     5581 ccggacaaac ctgcgtctgc gccaaccgtt tatacgtgca ggacggcgtg tatgaccgct
     5641 ttgccgaaaa attgcagcag gcggtgagca aactgcatat cggcgacggg ctggataaag
     5701 acgtcaccat cgggccgctg atcgatgaaa aagcggtagc aaaagtggaa gagcatattg
     5761 ccgatgcgct ggagaaaggc gcgcgcgtgg tttgcggcgg taaagcgcac gaacgcggcg
     5821 gcaacttctt ccagccgacc attctggtgg acgttccggc caacgccaaa gtgtcgaaag
     5881 aagagacgtt cggccccctc gccccgctgt tccgttttaa agatgaagcc gatgtgatcg
     5941 cgcaagccaa tgacaccgag tttggccttg ccgcctattt ctatgcccgt gatttaagtc
     6001 gcgtcttccg cgtgggcgag gcgctggagt acggcatcgt cggcatcaat accgggatta
     6061 tttccaatga agtggccccg ttcggcggca ttaaagcctc gggtctgggt cgtgaaggtt
     6121 cgaagtatgg catcgaagat tacttagaaa tcaaatatat gtgcatcggt ctttaactgg
     6181 agaacgcgaa tgagcagcaa taaagagtta atgcagcgcc gcagtcaggc aattcctcgt
     6241 ggcgttgggc aaattcaccc gattttcgct gaccgcgcgg aaaactgccg ggtgtgggac
     6301 gttgaaggcc gtgagtatct tgatttcgcg ggcgggattg cggtgctcaa taccgggcac
     6361 ctgcatccga aggtggttgc cgcggtggaa gcgcagttga aaaaactgtc gcacacctgc
     6421 ttccaggtgc tggcctacga gccgtatctg gagctgtgcg aaataatgaa tcagaaggtg
     6481 cctggcgatt tcgccaagaa aacactgctg gtcaccacag gttccgaagc ggtggaaaac
     6541 gcggtgaaaa tcgcccgcgc cgccaccaaa cgtagcggta ccatcgcctt tagcggcgcg
     6601 tatcacgggc gcacgcacta cacgctggcg ctgaccggca aggtgaatcc gtactctgcg
     6661 ggcatgggcc tgatgccggg ccatgtttat cgcgcgcttt atccttgccc gctgcacggc
     6721 atcagcgagg atgacgcgat cgccagtatc catcgaatct tcaaaaatga cgccgcgccg
     6781 gaagatatcg ccgccatcgt gattgagccg gttcagggcg aaggcggttt ctacgccgcg
     6841 acgccagcct ttatgcagcg tttacgcgcg ctgtgtgatg agcacgggat catgctgatt
     6901 gccgatgaag tgcagagcgg cgcggggcgt accggcacgc tgtttgcgat ggagcaaatg
     6961 ggcgtggcac cagatctcac cacctttgcg aaatcgatcg caggcggctt cccgctggcg
     7021 ggcgtcaccg ggcgcgccga agtgatggat gccgtcgctc caggcgggct gggcggcacc
     7081 tatgccggta atccgattgc ctgcgtggcg gcgctggaag tgttgaaggt gttcgagcag
     7141 gaaaatctgc tgcagaaagc caacgatctg gggcagaagc tgaaagacgg attgctggcg
     7201 atcgccgaaa aacacccgga gatcggcgac gtacgcggtc tgggggcgat gatcgccatc
     7261 gagctgtttg aagatggcga tcacagcaaa ccggacgcca aactcaccgc cgagatcgtg
     7321 gctcgcgccc gcgataaggg cctgattctt ctctcctgcg gcccgtatta caacgtgctg
     7381 cgcatcctgg taccgctcac tattgaagac gctcagattc gtcaaggtct ggagatcatc
     7441 ggccagtgtt ttgctgaggc gaagcagtag caccgtaccc atgccggagg cgacgctacg
     7501 cgtcttgtcc ggcctacggg gattctcgtc ggataaggcg tttacgccgc atccgacaat
     7561 ctgtacgtga acaggaagaa atctatgttg gccgggtaag gcggagccgc tctccggcaa
     7621 aaagaaccta taacaattaa acgcgtgacc cgacgcggga aatgtcgggg cgctctccca
     7681 agtgacacac tttcgagagg attcaggatg gggcaatcat cgcaaccaca tgagttaggc
     7741 ggcgggctga aatcacgcca cgtcaccatg ttgtctattg ccggtgttat cggcgcaagt
     7801 ctgtttgtcg gttcaagcgt cgctatcgcc gaagcgggcc cggcggtatt actggcctat
     7861 ctgttcgccg gattactggt ggttatgatt atgcggatgc tggcggaaat ggcggttgcc
     7921 acgcccgata ccggttcgtt ttccacctat gccgataaag ccattggccg ctgggcgggc
     7981 tataccatcg gctggctgta ctggtggttt tgggtactgg ttatcccact ggaagccaac
     8041 atcgccgcca tgatcctgca ctcgtgggtt ccaggcattc ccatctggtt attttccctc
     8101 gtcattaccc tcgccttaac tggcagtaat ttattaagcg ttaaaaacta cggcgaattt
     8161 gagttctggc tggcgctgtg caaagtcatc gctatcctgg cctttatttt ccttggtgca
     8221 gtcgcaatta gcggttttta cccgtatgcc gacgtgagcg gtatttcaag attgtgggat
     8281 agcggcggct ttatgcccaa cggcttcggc gcggtattaa gcgcgatgtt gatcaccatg
     8341 ttctcgttta tgggcgcaga aattgtcacc attgccgccg cggaatccga cacgccggaa
     8401 aaacatattg tccgcgccac caactcggtt atctggcgta tttctatctt ctatttatgc
     8461 tctatttttg tcgtcgtggc attaataccg tggaatatgc cggggctgaa agccgttggt
     8521 tcttatcgct cggttctgga attgctcaat attccccatg cgaaattaat catggactgc
     8581 gtgatattac tttccgtaac cagttgcctg aactcggcgc tgtataccgc ttccaggatg
     8641 ctctactcct taagccgtcg cggtgatgcg cccgcggtaa tgggtaaaat caaccgcagt
     8701 aaaaccccgt acgtggcggt gttactctcc accggcgcgg catttttaac ggtggtggtg
     8761 aactattacg cacctgcgaa agtgtttaaa ttcctgatag acagctccgg tgctatcgcc
     8821 ctgctggttt atttagtcat cgccgtttca cagttgcgga tgcgtaaaat tctgcgagca
     8881 gaaggaagcg aaattcgctt gcgcatgtgg ctttacccgt ggctcacctg gctggtaatc
     8941 ggctttatta cctttgtgtt ggtggtgatg ttattccgcc cggcgcaaca gttagaagtg
     9001 atctctaccg gcttattagc gatagggatt atctgtaccg tgccgattat ggcgcgctgg
     9061 aaaaagctgg tattgtggca aaaaacaccc gttcataata cgcgctgata atgatcagga
     9121 gtcacaccat gaccattacg tctctggatg gctatcgctg gctgaagaac gatattattc
     9181 gcggtaattt tcaaccggat gaaaaattac gaatgagttt gctgacatcg cgttatgcac
     9241 ttggcgttgg gccgttacgg gaagctcttt cgcaactggt ggcggaacgg ctggtcacgg
     9301 tggtgaatca aaaagggtat cgggtggcgt ctatgtcaga gcaggagttg ctcgatattt
     9361 tcgatgcccg cgccaatatg gaagcgatgt tagtgagtct ggcgattgcc cgcggtggcg
     9421 atgagtggga ggcagacgtt ctcgcaaaag cgcatctgtt gagtaagctt gaggcccgtg
     9481 acgccagcga gaaaatgctt gatgagtggg atctgcgtca tcaggcgttt catacggcaa
     9541 ttgtggcagg ctgtggttct cactatttgc tgcaaatgcg tgaacggttg tttgatctgg
     9601 cggcgcgcta tcgatttatc tggctgcgac aaacagtgct ttcggtggaa atgctggagg
     9661 ataaacacga tcagcaccag accctgactg cggcggtact ggcgcgagat accgcgcgcg
     9721 ccagtgagtt aatgcgccag catttactga cgccaattcc cattatccag caggcgatgg
     9781 ctggcaatta actactcttc cggaatacgc aacacttgcc ccgggtaaat tttatccggg
     9841 ctttttagca tcggtttatt cgcttcgaag attttattgt acagattagc gttaccgtag
     9901 acctgtttgg aaatggcact cagagtgtcg ccagacttaa cggtataaaa ctggctggca
     9961 gtggctggtg tcgccgtttt cacctgatca tcaacactgg caatgccgga aatattcccc
    10021 accgcaacaa ggattttctc cttcgcctcc tgactcaggc cgtcaccagt gaccgtcgct
    10081 ttgccgtcgg caatttgaat gttcacttta tcggcatccg gtataccggt tttgttcaga
    10141 tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct gacctgtaac cgcgtcccag
    10201 agtttttctc cggcatcttt cacaaaattg aacagaccca tagctacctc attcgttaac
    10261 ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt aacgccgcgg ggatgagtac
    10321 aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa accggggata tacccaagca
    10381 gtgtcagcag aatattaata atgaacgccc aaccgaaccc tttaccgagc agcacgccga
    10441 gcggcggcag aatgatggtg atgacgattc tccagaaacc catatgtact ccctataaga
    10501 aaattactca ttgtttaaaa agagatttta tctcttaagc gtagtaattt tagcggagcc
    10561 tgccagtttc agcttacgct tttactctca tttacgctgc tgaggctggt ata
//
LOCUS       DAZFFG010000142        10547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFFG010000142 DAZFFG010000000
VERSION     DAZFFG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.142"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..536
                     /locus_tag="ACHJ7J_004253"
     CDS             <1..536
                     /locus_tag="ACHJ7J_004253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002414.1"
                     /translation="ISEYIKQVYNFNKYVRKHTYISCWHANEFESAAMWDLYSKNDAS
                     VVIETTYAEIKNLLPPEAMIGLVKYIDYDKDVFPLDNTFYPFTHKRKSFEHEREVRFL
                     IQNLPITAEGTSLDFNNAVAPNVIPIDVDVSSFVKKVHLSPRAPEWMLNLLKDIVTKY
                     SINVEVRRSNLYISPIY"
     gene            complement(707..1756)
                     /locus_tag="ACHJ7J_004254"
     CDS             complement(707..1756)
                     /locus_tag="ACHJ7J_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5002415.1"
                     /translation="MAVRKLTTGKWLCECYPAGRNGRRVRKQFATKGEALAFERYTMG
                     EIEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAINLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLNTALHYNPLS
                     TMPTQHTEANDMLPD"
     gene            complement(1945..2136)
                     /locus_tag="ACHJ7J_004255"
     CDS             complement(1945..2136)
                     /locus_tag="ACHJ7J_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002416.1"
                     /translation="MLGKVFFVVLSCSLLLNPLTTYARNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
     gene            complement(2152..2721)
                     /locus_tag="ACHJ7J_004256"
     CDS             complement(2152..2721)
                     /locus_tag="ACHJ7J_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HFO5002417.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALCEDGFYIFD
                     REFLPSAFKNLFVITDNNSEFICDKEFDDIRDGKWVISIDGEITIRDITRLPGGRIFV
                     EGGNRAFECKIEDIEIIGKIISLTVKYVK"
     gene            2847..3068
                     /locus_tag="ACHJ7J_004257"
     CDS             2847..3068
                     /locus_tag="ACHJ7J_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO5002418.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            3101..3610
                     /locus_tag="ACHJ7J_004258"
     CDS             3101..3610
                     /locus_tag="ACHJ7J_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HFO5002419.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            3618..3914
                     /locus_tag="ACHJ7J_004259"
     CDS             3618..3914
                     /locus_tag="ACHJ7J_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage filamentation protein Fil family protein"
                     /protein_id="HFO5002420.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWITGEDGKRWHPCHSQDELL
                     SELTTRKRRKSKCMRQKVKWFISFVTEGRVIQYLKMICSVAIRHCRAMAVTFSR"
     gene            4032..4373
                     /locus_tag="ACHJ7J_004260"
     CDS             4032..4373
                     /locus_tag="ACHJ7J_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HFO5002421.1"
                     /translation="MAINGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            4441..4674
                     /locus_tag="ACHJ7J_004261"
     CDS             4441..4674
                     /locus_tag="ACHJ7J_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO5002422.1"
                     /translation="MRNIETRTTQTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            4674..4901
                     /locus_tag="ACHJ7J_004262"
     CDS             4674..4901
                     /locus_tag="ACHJ7J_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5002423.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            4898..5755
                     /locus_tag="ACHJ7J_004263"
     CDS             4898..5755
                     /locus_tag="ACHJ7J_004263"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO5002424.1"
                     /translation="MSTILKWAGNKTTIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVD
                     SSAVYGVRA"
     gene            5752..8166
                     /locus_tag="ACHJ7J_004264"
     CDS             5752..8166
                     /locus_tag="ACHJ7J_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5002425.1"
                     /translation="MSHADMNNCCGFNEAAASFSWNSPKKAINPYLDPVEVAPVSTLS
                     NLITLYAADNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRNTWERVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKVSPYVSYEAVLHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREEREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFASYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLYQQGKDKSADGDTRTDITRMDDKELHDYLHSMNKKERRELAARLRLVKPKRRR
                     DYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     KWRDLY"
     gene            8319..8507
                     /locus_tag="ACHJ7J_004265"
     CDS             8319..8507
                     /locus_tag="ACHJ7J_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002426.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            8518..8751
                     /locus_tag="ACHJ7J_004266"
     CDS             8518..8751
                     /locus_tag="ACHJ7J_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5002427.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            8994..10157
                     /locus_tag="ACHJ7J_004267"
     CDS             8994..10157
                     /locus_tag="ACHJ7J_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002428.1"
                     /translation="MDNGSITFFDITACGFYRLKNKPEELDYKFGDLMGVLDDLEGWL
                     KDKNFEQTLPWNKEEQPLRTRVYSRGLVRDSQTKDAVIVLYREVGNGNGIHGIKVGSK
                     VSGDSKGTIRAGREHGEDKIIWGEPCYYWIIPELNKIASIRFPHSFADTYLFAQYFIQ
                     HVNNNSKLGKRTKSKRTFESAKTPGRCVDVYNTKFQYKDGKNEINCIFKFVLEETKLK
                     AAEENFERLRHKITHTLIKDTTVINQNDTRQPLLKLTSVALASLVGEEKRNKLIGAPP
                     VLEQPRKIEVKIDGAPSSDELRNLFSMRGEESEWDVGFLLADRANPVWLSSYVARTKL
                     PLQDSDGFEHYSARFLLDEINKIRDDLISEVKYAEEASIKESEAASTKKIAEG"
     gene            10159..>10547
                     /locus_tag="ACHJ7J_004268"
     CDS             10159..>10547
                     /locus_tag="ACHJ7J_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002429.1"
                     /translation="MTETEGLIITVAKQQAINFVAVVLCCVISYFSFKSFSYNDAKDI
                     LGALINISASIFTIVGLWVGFLYPNAMNSIVKDDVSYIKNEHDSPRIEKLVYTIITSA
                     CVMAGILIFFLVKAVFYALPVYQANKD"
BASE COUNT         2930 a         2226 c         2533 g         2858 t
ORIGIN      
        1 ctatctctga gtacatcaag caggtgtata atttcaacaa gtatgtaaga aaacatactt
       61 atataagttg ttggcatgca aatgagtttg agtcagcagc gatgtgggat ctctatagta
      121 agaatgatgc ttccgttgta atcgaaacca cttatgcaga aataaaaaat ctattaccgc
      181 cggaagctat gattggcttg gttaagtaca ttgattatga taaggatgta ttcccgcttg
      241 ataatacatt ttatcctttt actcataaaa gaaaatcatt cgagcatgag agagaagtaa
      301 ggtttttaat tcaaaactta cctatcactg ctgagggtac tagcttggat tttaataatg
      361 ctgtagcgcc aaatgttatt ccgattgatg ttgatgttag ttcatttgtt aaaaaagtcc
      421 atctgtctcc ccgcgcacct gaatggatgc tgaatttgtt aaaagacatt gtgactaagt
      481 attcaataaa tgttgaggtt aggcgttcta atttatacat ttccccaatt tactaaacgg
      541 taaccataat taagtgatta tagtgctgga ggatattctc ttttaaaggg aatgtcatcc
      601 ggtctgtaat atttgcaaat taacacaata tggttggaaa aggccaaata attctcgggt
      661 agttgcatag tgaagtattg aatcccataa acggcccaca atttacttaa tcggggagca
      721 tatcattagc ttcggtatgc tgtgtgggca ttgtagataa tggattgtag tgtagcgctg
      781 tattaaggtg atccggtgca agatgagcgt aacgcattgt catttttata tcgtgatgtc
      841 cgaggatttt ttgcaaagca aggatatttc cacccgacat catgaagtgc gccgcaaacg
      901 tatggcgcag aacgtgtgtc agttgaccgc gagggagcac gattgacgtt ttttccatca
      961 cggataaaaa ttgaaaatag cagtctgtga agaaattgaa cccatcaagc gccatgatct
     1021 cttcgtaaag ctctttactg atagggatgc ttctgttttt cttccccttc gttcttacaa
     1081 aggtaattcg gtatttggtc acctgtgatc gggtaaggtt tattgcttct cgccagcgtg
     1141 cgcctgtgct taagcatatc ttgactacca gtgccagaat tgggtcctga cgtttgcaat
     1201 cagccagcaa ttcaacaatc tgctcatggg taagccatgc catctctttt tctgcgatgg
     1261 tgaattttcg catgttctcc agtgggttcg gatacgacca ttcgcccagg cgggatagtt
     1321 cgctaaaaac actacttaga tagctttgct ccaggttaat agtgaccggg cttgctcctt
     1381 tcttccattt ctcgctgaag tagatctctc ctgtcaggcg tttatctcga tagtgggcaa
     1441 acattttaga ggtgagatcg gttgcaaggg gattgccaag agcgtcaacc atcaacagca
     1501 atttgtcata aacatgctgc ccagcagtca gagatttacc atgtagtttg aaccatagct
     1561 caaccacgtc tttcaatgtc cgacgatcca ctgattcgcc cagccagggc tttgcttcta
     1621 tttcccccat ggtgtatcgc tcgaaggcca gtgcttcgcc tttggtggcg aattgtttac
     1681 gcacacgacg cccattacgt ccggcggggt aacattcgca aagccatttc cctgtggtga
     1741 gttttcgtac tgccataaaa aatgccctcc agtagagagc attttcactg tatgtataac
     1801 cagtgtcaat gtatgaaatc ctacgaccat acatctcact gaagccataa ttaagttgac
     1861 tattcttttt gctatgtgag catgtaactt ttgcggttaa cctgcggctc atttttattt
     1921 taggcgcaga tataaaagca aaagttatcg tgagttttta gtacagattt ttttggattt
     1981 actaatagtt ccatcattgc aaacgaattt gccatctgag gtacagtgag aaacacctcc
     2041 ctttttccct gaacagggat aatttctagc ataggtagtt agtgggttta ataacaaaga
     2101 acatgacaaa accacaaaaa ataccttacc aagcatagtt tcctcccggt actatttaac
     2161 atacttgact gttaaactta taattttacc aattatttca atgtcttcta tcttgcattc
     2221 gaaggctctg tttccaccct cgacgaagat tcttccaccg ggtaaacgag taatgtcacg
     2281 gatcgttatt tcgccatcaa tacttattac ccatttacca tcacgtatat catcaaattc
     2341 cttatcacaa ataaattcag aattattatc tgtgattaca aaaagattct tgaatgccga
     2401 cggtagaaat tctctatcga aaatataaaa accgtcttca cacaaagccc catcagataa
     2461 tacatattta gcaacttcca tagtatttgt attacctgaa gtttgctttg aaccatgccc
     2521 ggttgtgagc caattaagcg aagtgcctgt ttcaagggcg cactggatta cccattctgc
     2581 tgggaatgag tcacgcatgt agcgtgtggc gagtgtactt ttagagattc ctaaatgatc
     2641 gcacaacgcc tgtcgagtct tgaatccata agcttctacc atgcgctcta tagcgcctcg
     2701 tccgcctttc tccaaattca tggtcactcc aagtgaactt ttgtcttgac gatttcactg
     2761 tgcgatcgta tgtttatggt gttcacaaaa tacaaacgat ccgtattcgt cctgattaat
     2821 catcattaaa cgaggaatgt tgcatcatga gacctaacat ttcaatcact cttaccacgc
     2881 ctcatgtgac tattgaacgc tatagcgagc tgacagggct gtccatcgat actatcaatg
     2941 acatgttggc tgatggacgc cttatccgtc accgtttgcg caaagataaa aaacgcgaaa
     3001 aagtgatgat caacatagca gccatgaccg ttgatgcgct ttcagaatgc aatctaaacc
     3061 ttaattagtt cgattctgaa atacatcaga ggcattgact atgtttgatt accaagtttc
     3121 caaacatcca cattttgatg aagcctgtcg tgcattcgca ctgcgccaca acctggtgca
     3181 actggcagaa cgtgcaggca tgaatgtgca gattctgcgg aacaagctga acccagctca
     3241 acctcattta ttaaccgcac cagaaatctg gttgcttacc gatctgactg aagattcaac
     3301 gctagtagat ggttttctgg ctcagattca ctgcctgcca tgtgtaccga ttaatgaggt
     3361 ggcaaaagag aaactgccac attacgtcat gagtgcaacc gcagagatcg ggcgtgttgc
     3421 tgcaggtgcg gtatctggcg atgtaaaaac cagtgcaggc cgtcgtgatg ctatcagcag
     3481 cattaactct gtaacacgac tgatggcgtt ggctgctgtt tcattgcagg cccgtttaca
     3541 ggctaaccct gcgatggcga gtgcagttga taccgtgact ggcctcggtg cttcatttgg
     3601 tttgctgtga ggtgcttatg ctgacgaaag aaccatcatt tgcatctctg ctggtaaaac
     3661 aaagcccggc aatgcactac ggtcacggct ggatcacggg tgaggatgga aaacgctggc
     3721 atccatgtca ttcacaagat gaattgctgt ctgaattgac cacgaggaaa cggagaaagt
     3781 ccaaatgtat gcggcagaaa gtgaagtggt ttatcagttt cgttacagag gggagagtta
     3841 ttcagtacct gaagatgatt tgctctgttg ctatccgtca ttgtcgggcg atggcagtta
     3901 ctttttcacg ttaaaggatg ggacgttttt acggggagag caggttaaag agacgatacg
     3961 aaaaaatgta tctcctcttg agcgttaccg taagaacaaa gaacgatagc tgcgtttggg
     4021 ggatatgaag tatggcaatt aatggcgctg cagcaactgt tccattaagc cccggtgaac
     4081 gcctgaatgg acttaatcat atcgcggagt taagggcgaa agtttttggc cttaatattg
     4141 agtcagagct tgagcggttt attaaagata tgcgtgatcc tcgggatatc aataatgaac
     4201 aaaataaacg ggcactggct gccatattct ttatggcaaa aattccagct gaacgtcata
     4261 gcatcagcat taatgagctg accactgacg aaaagcggga gttgattaaa gcaatgaatc
     4321 attttcgtgc agtggtgagc ttatttccca gacggctaac catgccgaat taaccaaata
     4381 atgaaattaa tggcgtaaac ccgccgggca tccctttatc taaattcagg agaattgatt
     4441 atgcgtaata ttgaaacccg caccactcaa accggaccgg atgacgcagg gcttaatatt
     4501 ttactgacag aggctcgtct ggaagaacgc cgggcaaggg ctgaagcaat ggccgctcgc
     4561 cttgatagcc tggcgtgtca tatctcatcc cgccagttaa accacgtcga agcggcagaa
     4621 ctgctgcgtg tgactgctga agcaatccag aacgaagcgc aggagatcca ctaatggctg
     4681 atgcaatgga tctcgtacag cagcgcgttg aagaagaacg ccagcgccat atccgtgctg
     4741 cccgtgccaa aacgccgggc gtgtcccgcg tgctttgtat tgagtgtgaa gcaccaattc
     4801 cgccagcacg acgccgcgcc attccgggag tgcagctttg cattacctgt caggaaatcg
     4861 cagagctgaa aggcaaacat tacaacggag gtgctgtatg agcaccatcc tgaaatgggc
     4921 gggaaataaa accaccataa tgtccgaact gaaaaaacat cttcctgctg gcccgcgact
     4981 ggttgaacct ttcgcgggtt cctgtgctgt gatgatggag acggattatc ccagctatct
     5041 ggttgcggat attaatcctg atttaatcaa cctctataaa aaggttgccg ctgattgtga
     5101 atcgtttata tctcgcgcca gagttttatt tgagatcgca aacagggagg tggcttatta
     5161 caacataagg caggagttta attactcaac tgaaattact gatttcatga aagcggtata
     5221 tttcctgtat ctcaatcgtc acggttaccg tggtttatgt cgctataaca agagcgggca
     5281 tttcaacatt ccctacggta attataaaaa tccgtatttc cctgaaaaag aaattcgcgc
     5341 atttgcagaa aaggcccagc gagcaacgtt tatctgcgcc agctttgatg aaacgctggc
     5401 gatgttgaag gcgggggatg tggtgtattg cgatccgccg tatgacggta cgttttccgg
     5461 ctatcacact gacggcttca ctgaagatga ccagtatcac ctagcatctg ttcttgaaca
     5521 tcggtcatca gaaggacatc cggtcattgt ttctaacagt gacacatccc tgatccgttc
     5581 gctgtatcgc aattttactc accactacat caaggcaaaa cgcagcatcg gtgtggcagc
     5641 tggcgagggt aaatcagcaa cagaaatcat tgctgtttcc gggccgcgct gctgggtggg
     5701 atttgattat tcgcgtggcg tggatagttc tgccgtgtac ggagtacgtg catgagtcat
     5761 gccgatatga acaactgctg cggctttaac gaagctgccg catcgttctc atggaacagc
     5821 ccgaaaaagg ccattaaccc ttatctggac ccggtggaag ttgcgccggt ttctacgctt
     5881 tcaaacctga tcactctgta cgctgccgat aacgagcagg aacagctgcg ccgtgaggcg
     5941 ctgagtgatc aggtctggga gcgttatttc tttaatgaat cccgtgatcc tgtccagcgc
     6001 gaaatggaac aggataagct cattagtcgg gcaaagctgg cgcatgagca gcagcgtttt
     6061 aatccggata tggtcattct ggcggacgtc aacgcccagc cttcccatat cagcaagccg
     6121 ctaatgcaac gtattgaata cttcagcagc ctgggcaggc caaaggctta ttcccgctat
     6181 ttacgtgaga cgattaagcc atgtctggaa cgactggagc atgtacgcga cagtcagcta
     6241 tctgcatctt ttcgctttat ggcaagccat gaagggctgg acggcctgct gatcctgcct
     6301 gaaatgagtc aggatcaggt gaaacgcctg tccaccctgg tagctgcgca tatgagtatg
     6361 tgccttgatg ccgcttgtgg cgatttgtat gccaccgatg acgttaagcc agaagaaatc
     6421 cgcaatacat gggaaagggt ggcagcggaa accctgcgtc tggatgtcat cccgcctgcg
     6481 tttgagcaac tccgtcggaa aagaaaccgc cgtaaacccg tgccctatga actcattccg
     6541 ggttcgctgg cgcgtatgtt gtgcgccgac tggtggtatc ggaaattatg gaagatgcgt
     6601 tgcgaatggc gggaagagca gttgcgtgct gtctgccttg tcagcaaaaa agtatctccc
     6661 tatgtcagct atgaagccgt gttgcataaa cgtgagcagc gccgtaagtc gctggagttt
     6721 ttccgttctc atgaactggt gaacgaagac ggcgacacgc tggacatgga ggatgtggta
     6781 aacgccagca gcagcaaccc tgcgcatcgc cgcaatgaga tgatggcctg tgttaaaggt
     6841 ctggagctta tcgcggaaat gcgcggtgac tgcgccgttt tctacaccat cacctgtccg
     6901 tcacgtttcc attccacgct aaataacggc agaccaaacc cgacctggac aaacgcgacg
     6961 gtaagacaaa gcagtgatta tctggtcggc atgtttgctg catttcgtaa agcgatgcac
     7021 aaagccggat tgcgctggta tggcgtgcgg gtggctgagc cgcatcatga cggtacagtt
     7081 cactggcacc tgttgtgttt tatgcgcaaa aaagatcgcc gagccattac tgctttgttg
     7141 cgtaagtttg ccattcgtga agagcgcgag gagctgggta ataacacggg accacgcttt
     7201 aagtctgagc tgataaaccc gcgcaaaggt acgccaacaa gctacatcgc gaaatacatc
     7261 agtaagaaca ttgacgggcg tggtctggct ggtgagatca gcaaggaaac gggtaaatcc
     7321 ctgcgtgata acgctgaata cgttaatgcc tgggcgtctc tgcatcgtgt tcagcaattc
     7381 cgcttctttg gcattccggg gcgtcaggct taccgtgaac tgcgattgct ggctggtcag
     7441 gcggcaaggc aacaggggga caaaaaagca ggtgcgccgg tactggataa cccgcgcctt
     7501 gatgccatcc tggctgctgc tgatgctggt tgttttgcct cctacatcat gaagcagggc
     7561 ggcgtactgg ttccccgtaa atatcacctc atcagaaccg cttatgaaat caacgaagag
     7621 ccgaccgcct atggcgatca cggcattcgt atttatggca tctggtcacc cattgcagag
     7681 ggcaagatct gcactcatgc agtgaagtgg aaaatggttc gtaaagccgt tgacgttcag
     7741 gaggcggcag ccgaccaggg cgcttgcgcc ccttggactc gtggcaataa ctgtcccctt
     7801 gctgaaaatt tgtaccaaca agggaaagac aaatcagctg atggggatac cagaacggat
     7861 atcacccgca tggatgacaa ggaattgcac gattacctgc acagtatgaa caaaaaagag
     7921 cgccgggaac tggctgcaag gttacgcctg gtgaaaccga aacggcgtag agactacaaa
     7981 cagcgaatta cagatcatca gcgacagcag ctcgtctatg aactgaagtc cagaggattt
     8041 gatggcagcg agaaagaggt cgatttactc cttcgcggtg gcagtattcc gtcaggagca
     8101 ggcctgcgta tcttctatcg gaaccagcgt ttgcaggagg atgataagtg gcgggatctg
     8161 tattaattac gcgggttaac aattcgtgct cttaataata ccaggcatat caggctgatg
     8221 aacgtaaaaa aacgttttac atcagtaaga ttattatata ctgtaaatat aaacagtggt
     8281 tatgtataca gtattgcttt ggtgtcatag gaggaaagat gcaggactat tttttggagt
     8341 ctttgaagct ccagcgcatt gatttttttc ttaagcttgt agcggctagt gagtgtagtg
     8401 atgaagagaa ggggctggcc ctgcagtggg tttctgaact gacagatgaa ctcatggcaa
     8461 aaatcagaac ccatgaatac aaccgctcaa tggatgtcat cagctgaggt gacttttatg
     8521 cgcattgaaa taatgatcga taaagagcag aagattagcc agtctacact ggacgccctt
     8581 gagtccgagc tttaccgcaa tctgcgcccc ctgtatccca aaacggtaat tcgtatccgc
     8641 aaaggtagct ctaacggtgt ggaactgacc ggactgcaac tggacgaaga aagaaaacaa
     8701 gtgatgaaaa ttatgcagaa ggtctgggag gacgacagct ggttacacta gcgaacgttg
     8761 cggacgataa aactggtttt tatcgtccgc aaggttgaac aacgagccgc gtgaggcgtt
     8821 agaaacgggt agtgaaaagt gatttaagaa tgccttgttc ccctgaaact ttggcctatg
     8881 caacaacatt cgacagcttt cttgattatc ataagtaact tgaagttcag ggacgattct
     8941 gatggtatac gtgaaaaatg tatcgtgtgt tgctttggca aaaaggagtt ttagtggaca
     9001 acggctcaat caccttcttt gatattactg cttgtgggtt ttatcgttta aagaataaac
     9061 ccgaagagct tgattataaa ttcggcgatc ttatgggggt tttggacgat ttagaagggt
     9121 ggctcaagga taaaaatttt gagcagactc tcccatggaa taaagaagaa caacctctgc
     9181 gaacacgagt ttacagtcga ggccttgtga gggacagcca gactaaagat gcagtaattg
     9241 ttttataccg tgaagtgggg aacggtaacg gaatacatgg tattaaggtc ggttctaaag
     9301 taagtggtga tagtaaagga accattaggg cgggaagaga acatggtgag gataaaataa
     9361 tttggggtga accctgctat tactggatta ttcctgagtt gaataaaata gcatcaatca
     9421 gattccccca ttctttcgct gatacttatc tcttcgcaca atacttcatt cagcatgtaa
     9481 ataataatag taagttaggt aaaagaacga aatctaagcg tacatttgaa tcagctaaga
     9541 cccctggtcg ttgtgtggat gtctacaata ctaagtttca atataaagat ggcaaaaatg
     9601 agataaactg tatcttcaag tttgtattgg aagaaacaaa acttaaagcc gctgaggaaa
     9661 actttgaaag gttaaggcat aaaattacac acacattgat aaaggataca acggtaataa
     9721 accaaaatga cacaaggcaa ccattgttga agctaacaag tgtagcttta gcatctctgg
     9781 tgggtgaaga aaagcgtaat aagcttattg gggcacctcc tgttttggag cagcctcgta
     9841 aaattgaagt taaaattgac ggtgcaccat catcggatga gcttcgaaat ctcttctcaa
     9901 tgagaggtga agagtctgaa tgggatgtgg gttttttact ggctgatcgt gctaatccgg
     9961 tttggctcag tagctatgta gccaggacta aacttcctct tcaagattcg gatgggtttg
    10021 agcattattc agcgcgtttt ttactggatg aaataaacaa aattcgtgat gatttgatta
    10081 gtgaagtaaa atatgctgag gaagcatcca ttaaagaatc tgaagctgca tccacaaaaa
    10141 agatagcgga aggttgatat gacagaaacg gaaggtttga taataacagt tgcaaaacag
    10201 caggcgatta attttgttgc tgttgttttg tgctgcgtta taagctattt ttcgttcaaa
    10261 agtttttcct ataatgatgc aaaagatatt ttaggtgcgc ttataaatat ttcagcatct
    10321 atattcacta ttgtcggttt atgggtcggt tttttatatc cgaatgcaat gaatagtatt
    10381 gttaaggatg atgtaagtta tataaaaaat gagcatgact caccaagaat agagaaatta
    10441 gtttatacaa taatcacatc tgcttgtgtt atggctggga ttctaatttt ctttttggtt
    10501 aaggcagtgt tttatgcatt acctgtttat caagctaata aagactt
//
LOCUS       DAZFFG010000143        10415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFFG010000143 DAZFFG010000000
VERSION     DAZFFG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.143"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            307..1194
                     /locus_tag="ACHJ7J_004269"
                     /pseudo
     CDS             307..1194
                     /locus_tag="ACHJ7J_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1207..2121
                     /locus_tag="ACHJ7J_004270"
     CDS             1207..2121
                     /locus_tag="ACHJ7J_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5002430.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLTCEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKNRMAKLFNQSDTSVRNKYRPAFDKWNELV
                     DKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2795..3013
                     /gene="yiiE"
                     /locus_tag="ACHJ7J_004271"
     CDS             2795..3013
                     /gene="yiiE"
                     /locus_tag="ACHJ7J_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5002431.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            3231..3473
                     /gene="yiiF"
                     /locus_tag="ACHJ7J_004272"
     CDS             3231..3473
                     /gene="yiiF"
                     /locus_tag="ACHJ7J_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO5002432.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGREEDGVEYQRKLREEW"
     gene            complement(3803..4732)
                     /gene="fdhE"
                     /locus_tag="ACHJ7J_004273"
     CDS             complement(3803..4732)
                     /gene="fdhE"
                     /locus_tag="ACHJ7J_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO5002433.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4729..5364)
                     /gene="fdoI"
                     /locus_tag="ACHJ7J_004274"
     CDS             complement(4729..5364)
                     /gene="fdoI"
                     /locus_tag="ACHJ7J_004274"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5002434.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5361..6263)
                     /gene="fdoH"
                     /locus_tag="ACHJ7J_004275"
     CDS             complement(5361..6263)
                     /gene="fdoH"
                     /locus_tag="ACHJ7J_004275"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO5002435.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(6276..9326)
                     /gene="fdnG"
                     /locus_tag="ACHJ7J_004276"
     CDS             complement(6276..9326)
                     /gene="fdnG"
                     /locus_tag="ACHJ7J_004276"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8739..8741),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5002436.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9520..10353
                     /gene="fdhD"
                     /locus_tag="ACHJ7J_004277"
     CDS             9520..10353
                     /gene="fdhD"
                     /locus_tag="ACHJ7J_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO5002437.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2639 a         2481 c         2631 g         2664 t
ORIGIN      
        1 ttggctccat acgtgtcttg ctacatcact gttcaggagc atttaaatat tatttgtaga
       61 gctgtatggc aattatcgca gataatgctt aattcatcat cttacttcag atttgagcga
      121 tttttgatag tcaaaacact ctctggtgcc atacttttct cataaatatt tttaaaatct
      181 atagctaaca aatattatgc aaagataatg gactagcatc attgtcagta tcattcattt
      241 actgctctgg atgttataaa taattatgta gcatgtttgc tcttaaaccg tgggaaggag
      301 actgtaatgg ttgtcaatat taatctcatt aaacaagaaa gttattctgt tgttaattta
      361 gaaaaacaac taatgagtaa tgaatcaccg aagcaaacgc tattaaatga tttttcacat
      421 cgccttaaaa tggaaggtga ggaacaactg ttccattgtg ttaacgaact gaaatatggt
      481 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
      541 aaagtaccag aaagcgcagc cctggcaatg acgggaacag tatataaatt atttggtagt
      601 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      661 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      721 aaggatggca gcctccagag taagcactac acatttgatg taggtgaagt tataaacagt
      781 tataatattg acctctttgt gaatgcttcg agtattaatc aaacttactt acatgataaa
      841 aatcctggtg acgttattag cctgtatggc actttagata acattcccct tgtagttaag
      901 gaatgcacgg gtaaaaatca tcaaattaac gttaaagcca aattgccaca agaacgaagt
      961 gaaaaagttg aggatatgtg tgaaagaatg agaggtggca tctctatgtt taatcacacc
     1021 acaaaaacag ctggtaatat agagcataat ttgcagctaa gttttctcgt gggttcacga
     1081 ccgtcgatca ccaataaata ctcagccaaa gatgtagcgc cagacatact tgtattgccc
     1141 gtcaacgtat atcatgcgga ttttaaaatt aaaatcaatg acgcagtaga gaaggcttcg
     1201 tcaattgtgt tatgtgctgt gcaaaaaaat ctccatgcag caagaaaggt tctcagcggg
     1261 ataataggaa atgtgccaaa aaataataaa ccaaaaacaa tcatttttga tttacgtttt
     1321 cttagtgcaa ttaagcagaa agtttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
     1381 acggcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
     1441 acctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     1501 atggcgacag gtgtgggcgc ggctttaatg tatgcaacat catcaggacc gtattatgcg
     1561 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc taaattgttt
     1621 aaccagtcgg atacttctgt gcgcaacaaa tatcgcccgg cttttgataa atggaatgaa
     1681 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     1741 ttagcgacag cgataaatga aactcctgag ggggatacga atgttgccgt tgtgatgggg
     1801 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     1861 ctctttgaaa aacgcctcgc cgatgatgga gaaatagaaa acctttttga tcaacaggga
     1921 tattttagtt gcgattcatt aacagccgaa gaagtaatga tgttaaaaga cctttttgat
     1981 attcgagttc ttcatctctc taacaaattt aatgttgggt tgcaaggtaa tatcaacacg
     2041 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaatc taactcgtat
     2101 accgtaggga tgggagtctg aagagtaaaa ctcattagga ttatcagaat tcggccaatg
     2161 cattgcaaaa atgaaataca aagagagatg tgtgcaggcg cttagccgcg aggggcatgg
     2221 cgaagccata cggaaagcac cagcattcag aactcctgtg aaagcggcat agtgccgctg
     2281 ctcaggagtt ttgttatgac ccgtatcgtg ctgagcgttg ccaccctatg atgcgattaa
     2341 aaaaactcac cgtcgcgctg atgattgacg tcaatgctat cggatgatgc cgggggtgtt
     2401 ccggaaggaa ttccttgttt cccgaactgt taccaacaaa attataacga tgagcaagta
     2461 aagaccggtt ggtggcagta ctgaccggtc tgtgatggaa gattaatttc accttaaatc
     2521 ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg caaataaata
     2581 tccctgaata taatatattc atagcaccca taactcatcc tgggcgataa ctaaaccatt
     2641 tttgagtaat aaatgaaaca actgcaacgc tcttattatt tctggaaaac gtctcgcaaa
     2701 aacaaaacaa catgcaaaaa tccgctttcg ttaattaatt ttccggcgta attttcgcac
     2761 atacattaat gagtaatata tgtaaggtgt ggtaatggcg atgaatacgg tttttcttca
     2821 tttatcagaa gaggcaatta aacggctgaa caagcttcgg ggctggcgta aggtttcacg
     2881 atctgcaatt ttacgcgaag cggtagagca atatctggaa cgtcagcaat ttccggtacg
     2941 taaagcaaag ggaggcaggc aaaaggggga ggtagttggt gttgatgatc agtgtaagga
     3001 gcataaggaa tgacaaaaat ttttcattct tgaatataaa aaaacagatg cccttattct
     3061 ggtattaata caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcatatc
     3121 tggaaaacgc ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcga
     3181 caagggtact tttctctcca tagatatcaa atgatattgg tcaaatattt atgaactctc
     3241 tggcaggtat tgatatgggc agaattatac tcgatttatc gaatgaggtg attaagcaac
     3301 ttgatgatct tgaggtgcag cgtaatcttc ctcgtacgga gctattacgg gaagcggtag
     3361 atcaatactt ggtaaatcaa tcgcaaacag taagaaccag tgcttttggc atatggcaag
     3421 ggcgtgagga agatggtgtc gaataccagc gtaagctgcg cgaggaatgg taatgggaaa
     3481 aaatgatatc aatcagattg ctgacaacgt gtgcgttgtt catgccggat gcggcgtgaa
     3541 cgccttaccc ggcctacaaa gtcgtataaa ttcaatgtat tgcagtttat tggtaggcct
     3601 aataagcgcg gcgcatcagg caatcttacg cttatcatca gtctcatacc cataacctta
     3661 ttgatgtggg ggtgggtaaa ttgaggcaaa acaggcactg gcggcatagc ttgaggtggt
     3721 aacgcggaat acgagagatg gtgtcgtcac accctatgag ctgtaatccg ggcgaccagc
     3781 gccacccgga aaacgtcaga gattactccc cttcacccgg aaacaggaac gggttgatgg
     3841 aactgcgggc atagccttct tgctccattc gtgcgtccag taccagagag gcgaggtcat
     3901 ctgccacggc ttcaactttc ggttcttttt cctgatagag aattttcagg taagtgccgc
     3961 agtcatcgca gctttcggct ttaatcgcgg cctgttcgtc atccagcgac cagtaatgca
     4021 gtttgccgct ctgctcgcag ttgctgcatt ttacgcgcac aacgtgccat tcagtttcac
     4081 acaggttgca gtgcaggtaa cgtagcccct gagtggtgcc aatttgcacc atgctggaca
     4141 ccggcataga gccacatacc gggcaatatt gacgttgttc gccgtattca gcgcgggctt
     4201 tgccggggat cagattggcc atctgcgccc agtagagcga cagtgcagcc cagataaacg
     4261 gcgctttatc gctactgacg gatgagaaat cagaggcaaa cagtgcgctg gccatatctt
     4321 ccagctcctg agtcgatgcc ttctccagat tctcaatcac tgccagcgcc gggccgctca
     4381 tttcaggttt cagctcagca attagcgcca tcagcagctt ttgccagtgc ttatcacgcg
     4441 gcagaacgtg aatatccagc gggggcttgc cttgtgcgct ggcttcttta atgcgtgcag
     4501 tcagatccat ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg gcgataagcg
     4561 cagcaaagcg caggtaatca cccagcggat tattttctgc caactcgcgc agacgctcgg
     4621 cgcggcggtt gtataaattc ttgagccgag ggaacaataa cggcggaatc atatccgccg
     4681 tacgtttctc gctcgaaccc agctcatctt gcgggattat gcgaatactc attcagcttt
     4741 cttttccgtt gtcttgcgga cctcacggta ccagcgcggg tgatgtttct tcgcccaggc
     4801 gctggtaacc catccttcca ccatggcggt aatcgtgcct ttcacccaaa gggcggcgta
     4861 gatatgcacc atgataacca caattaacgc cactgcggca aatgaatgca gcattaacgc
     4921 gaatcggatc accgggattg agaaagcagg cgcaaaataa ggacgccaga taatcacgcc
     4981 gctcaccagc agcagaacca ggaaaataat cgccgcccag aaaacgcatt tctgaccgaa
     5041 gttataacgc ccggtgtcac ctacttcctc gttgacgacg atcttacgaa tattcttcgc
     5101 ccaaaagata tcatcccgat tgattaggtt gtgatgccag taacggaaaa acatgatgat
     5161 gaacgaggca aacataacca cgccgacaaa cgggtgcaga attcgcgcca gctgcggtgt
     5221 gcccatgatt tgcatcaacc agttgaagga cgggaacaaa aagcccagcc cgctcaccgc
     5281 cgccaggatg aagcagaagg cggtgatcca gtggttgata cgttccggcg cggtgtagcg
     5341 cacgatggtg tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg cagattattc
     5401 tcttcctcat ccgcacggtt cggaccgaca cccacgtagt ggaagatact ggctgcgaag
     5461 gtagccgcaa agccaacagc tgcgagcggt ttccagatgc ctttccagaa tttcacggtt
     5521 tcgctgattt ccgggttctc cggcaagcca tgatacagat ttggcttgtc agcatggtgc
     5581 agcacgtaca taacgtgtgt accaccgacg cctgccggat cgtacaggcc cgcattgtcg
     5641 taaccgcggg ttttcagctc cgccacgcgc tcgctcgcca gcgttttcat cgactctttc
     5701 gtaccgaagt gaatcgcgcc cgttgggcag gtcttcacgc aggctggttc ttgcccaacc
     5761 accacgcggt caacgcacag cgtacatttg tagacgcggt tgtcttccgg gttgaggcgc
     5821 ggaatgtcga acggacagcc cgcaatgcaa taaccgcagc caatgcactg ctcggactgg
     5881 aagtcgacga taccgttggc atactgaata attgcacctt ccgccgggca cgctttcaga
     5941 cagcctggat cggaacagtg catacagccg tctttgcgga tcagccattc cagtttgtcg
     6001 ttctgctcca cttccgagaa gcgcatcacc gtccacgatt tggcacttaa atcattgggg
     6061 ttgtcgtaca ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca ctctgaacac
     6121 gccacctgac aggctttaca gccgatacag gtggtgacgt cgatgagttt cgccacttct
     6181 tcctggaagt cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga acgacgaatg
     6241 atatcttgcg attgataagc catatgtcgt ctccgttaca ccttttccac attcacaagg
     6301 aaggacttaa actccggcgt ctgcgtgttt gcatcaccaa cgaatggcgt taacgtattg
     6361 gcaataaagc cttttttagc tacgccttca tagccccagt gaatcggaat accgatggta
     6421 tcgatatctt tgccgttcgc tttcagcgtg cgaatacgtt tggtcaccac cgctttggct
     6481 ttgatatagc cacggttgga ggagactttc acggtatcgc cctgggcaat gccaagttta
     6541 ttcgccagcg actccccgat ttccacaaac tgctctggct gcaaaatcgc gttcaacagc
     6601 gcgtgtttgg tccagtagtg gaagtgctcg gtcagacgat aggtggttcc gacatacggg
     6661 aacttatcgg ctttacccaa tgcttcggca tcgtctttaa agatacgtgc agccgggttc
     6721 gagataacgt ttggatgcag cgggttagtt cccagcggcg tttcaaacgg ctcgtagtgt
     6781 tccgggaacg gaccttccgc catcttatcg agggcaaaca gacgccccat gccttcctgc
     6841 tgcatgataa acggcccgac gccgctaccc ggaggcgctg cgctgtagtc cggaatatcc
     6901 cagccggtcc acttagtgcc gtcccatttc agcaactgac gcttcggatc ccacgggtta
     6961 ccctgcggat ctgcggaggc gcggttatac agaatgcggc ggttaagcgg ccatgcccat
     7021 gcccagccca gcgtgttacc gaggccagac ggatcggcgt tatcacggcg tgccatctgg
     7081 ttgccttccg gcgtccagct accggcgaaa atccagcagc cacaggaggt tgtaccgtca
     7141 tcgcgcaatt gggcgaacga gctaagttgt tggcctttct tgacgataac cgcaccggtt
     7201 gccggatcgg taatatcggc cagcgcctta ccgttgctct ccatcgctac ttcttccgac
     7261 ttcggctcat gcggaatggc gtagttccag gtcatgttca gcacctggtc cgggttcgcg
     7321 ccaccctgtt cggcatacat cttgcgcaag cgcaggaaga taccggagag gatctcgccg
     7381 tcagtcagcg caatccccgg ggcgtccgca cctttccagt gccactgtaa ccagcgacct
     7441 gagttaacga tagaaccgtt ctcttccgcg aagcaggtcg atggcagacg gaacacttcg
     7501 gtctggatct tcgacgagtc aacttcgttc agctcaccgt ggttctgcca gaagttagag
     7561 gtttcagtgt tcagcgggtc gatggtgacg aggaacttca gtttcgacag acaaccgatc
     7621 actttgtttt tgttcgggaa tgaggcaaca gggttaaagc cctggcagat atagccattg
     7681 accttgccct ctttcatcat ctcgaagtat tgcaggacgt cgtagccttt atcccacttc
     7741 ggcaaccagt caaagcccca gctattttcc gccgtcgctt tatcaccaaa gaaggccttc
     7801 atcatggaga cgaagaattt cgggtagttg ccccagtagt ttacctggcc ttccagcagc
     7861 ggttttggcg tgttggcggt aaggtaggtt tgcagatcgg tctgcttctc gcttggcagc
     7921 gtcatgtaac ctggcaggct ctgcgacagc agccccaggt ccgtcagccc ctgaatattg
     7981 gagtgaccgc gcagggcgtt aacgccgccg ccagccatcc ccatgttacc gagcagcagc
     8041 tggatcatcg ccatcgtacg aatgttttgc gcaccaacgg agtgttgcgt ccagccgagg
     8101 gcatacagga acgaggcagt tttatcgtga gcactggttt ctgcgatgta ttcgcagact
     8161 ttcaggaacg cgtcttttgg cgtaccacag atgttttcaa ccacatctgg cgtgtagcgg
     8221 gaaacgtgct gtttcagcaa gttccacacg cagcgcggat gttgcagcgt ggtatcgcgt
     8281 ttggcgaagc cgttttcgtc cagttcgtaa gtccaggagg atttatcgta cttgcgtttt
     8341 tccgcgtcgt agccggtgaa caggccatct tcaaagccgt aatcctcacg cacgatcagg
     8401 ctggcgttgg tataggcttc ggtgtattcg cggttgaatt tttcattgtt cagcaggtac
     8461 agcaatacgc ctgacaggaa agcaatgtca gtaccggaac gaataggggc atagtagtca
     8521 gccaccgcag ccgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc gccattgtga
     8581 attttggctt ccatcgccca gcggaacccg accgggtgag cttcagcggc gttaccgccc
     8641 atcaccacga cgaggttggc gttcttgatg tcgacccagt ggttggtcat cgcaccgcga
     8701 ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc ctggttgtcg
     8761 accgcgagca tacccagcgc gcgggagaat ttttgcgtta aatagccggt ttcgttactc
     8821 gacgcggaag cacacagcat cccggtggag agccagcggt taacagtcac gccttcggcg
     8881 ttttgcgcaa tgtagttagc atcgcggtct tctttcatca gtttggcgat gcgatcaaac
     8941 gcctcttccc aactgatttg ctgccattta tcagaacctg gcgcacggta ttccggaaac
     9001 ttcagacggc tttcggagtg gatgaaatcc accaggccag cgcctttcgg acaaagtgca
     9061 ccgcggttga ccgggtgatc cggatcgcct tcgatatgga agatagatgc tttggcgttt
     9121 tttgctccgt caccgaggct gtacatcaac agcccacagc ctacggaaca ataggtgcag
     9181 gtattacggg tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc gagtgctacg
     9241 ctgggtgcaa aacccagtgc cgctgccgtg gtgcctgcca taccgccagc gcagatctta
     9301 aagaactgcc ttctgctgac ctgcatggat tgctccttgt ttcgacattg tcacgtccca
     9361 tttacattcg cttgctgcgt gtgcagggag tgggagttat ttttctttgc ggaaggggcc
     9421 gcaaaggtcc agaattggct caatttccct ccatccagga agggtttgta acagaatacc
     9481 ataatgttgg tgtgtgtgtt cttatctggt taagagaaag tgaaaaaaac acagcaaaaa
     9541 gaaatcgaaa atgtgacaaa tatcacaggt gttcgtcaaa ttgagttatg gcggcgtgat
     9601 gatttacaac acccacggtt agatgaggtc gcggaagaag ttcccgttgc gctggtctac
     9661 aacggcattt cgcatgtggt gatgatggcg tcgcccaaag accttgagta ctttgcgctc
     9721 ggtttttcgc tttccgaggg gataatagaa agtccgcgcg atatcttcgg catggatgtc
     9781 gttccttcct gtaatggtct tgaagtacaa attgagcttt ccagccgccg ctttatgggg
     9841 ttgaaggagc gccgccgggc gctggcggga cgtacgggct gtggcgtatg cggcgtggag
     9901 caacttaatg acatcggaaa accggtgcag ccgctaccgt tcacccagac gtttgatctc
     9961 aacaaactgg atgatgcatt acgtcatctc aacgattttc agccagtggg acaactgact
    10021 ggttgtactc acgccgctgc ctggatgttg ccatctggcg aactggtcgg cgggcatgaa
    10081 gacgtgggtc gccatgtggc gctggacaaa ctgttaggcc gccggtcaca agaaggggaa
    10141 agctggcagc aaggtgcggt actggtttcc agtcgtgcca gttatgaaat ggtgcaaaag
    10201 tcggcgatgt gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac acttgccgtg
    10261 gaagtggccg agcgctgtaa tctgacgctg gtgggctttt gtaaaccggg gcgggcaacg
    10321 gtttatacgc atccgcagcg tttgagcaat taattaaaat aagtcctgcg atatattaaa
    10381 ttgcaggact tattcatttc gtgaatttta ttgtt
//
LOCUS       DAZFFG010000144        10304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFFG010000144 DAZFFG010000000
VERSION     DAZFFG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.144"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            153..1835
                     /gene="ydeN"
                     /locus_tag="ACHJ7J_004278"
     CDS             153..1835
                     /gene="ydeN"
                     /locus_tag="ACHJ7J_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5002438.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1887..3044
                     /gene="ydeM"
                     /locus_tag="ACHJ7J_004279"
     CDS             1887..3044
                     /gene="ydeM"
                     /locus_tag="ACHJ7J_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5002439.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFTKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEVFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3010..3132)
                     /locus_tag="ACHJ7J_004280"
     CDS             complement(3010..3132)
                     /locus_tag="ACHJ7J_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002440.1"
                     /translation="MTKHPTGIYVGCLVKAIRRRLRLEQRGSVINYSPFVLQHP"
     gene            3334..5019
                     /gene="yddA"
                     /locus_tag="ACHJ7J_004281"
     CDS             3334..5019
                     /gene="yddA"
                     /locus_tag="ACHJ7J_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO5002441.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFIGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEILHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            5057..7429
                     /gene="yddB"
                     /locus_tag="ACHJ7J_004282"
     CDS             5057..7429
                     /gene="yddB"
                     /locus_tag="ACHJ7J_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO5002442.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKANAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7486..10269
                     /gene="pqqL"
                     /locus_tag="ACHJ7J_004283"
     CDS             7486..10269
                     /gene="pqqL"
                     /locus_tag="ACHJ7J_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5002443.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFELMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVR
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3017 a         2284 c         2263 g         2740 t
ORIGIN      
        1 gaacagaaag taatgtcaac gctttatgga ctgttatttc ccttttttta gctaaatctg
       61 ctatctcttt atgtgactaa cttcacttac atccacttat ttctcttcgt aaaattactt
      121 tggaattaag tacaataaga agaggaacat ttatgaagtc tgcattaaag aaaagtgtcg
      181 taagtacctc gatatctttg atactggcat ctggtatggc tgcatttgct gctcatgcag
      241 cagatgatgt aaagctgaaa gcaaccaaaa caaacgttgc tttctcagac tttacgccga
      301 cagaatacag taccaaagga aagccaaata ttatcgtact gaccatggat gatcttggtt
      361 atggacaact tccttttgat aagggatctt ttgacccaaa aacaatggaa aatcgtgaag
      421 ttgtcgatac ctacaaaata gggatagata aagccattga agctgcacaa aaatcaacgc
      481 cgacgctcct ttcattaatg gatgaaggcg tacgttttac taacggctat gtggcacacg
      541 gtgtttccgg cccctcccgc gccgcaataa tgaccggtcg agctcccgcc cgctttggtg
      601 tctattccaa taccgatgct caggatggta ttccgctcac agaaactttc ttgcctgaat
      661 tattccagaa tcatggttat tacactgcag cagtaggtaa atggcacttg tcaaaaatca
      721 gtaatgtgcc ggtaccggaa gataaacaaa cacgtgacta tcatgacaac ttcaccacat
      781 tttctgcgga agaatggcaa ccgcaaaacc gtggctttga ttactttatg ggattccacg
      841 ctgcaggaac ggcatattac aactcccctt cactgttcaa aaatcgtgaa cgcgtccccg
      901 caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt gatcgtgcca
      961 aaacacttga ccagcctttt atgctttacc tggcttataa tgctccgcac ctgccaaatg
     1021 ataatcctgc accggatcaa tatcagaagc aatttaatac cggtagtcaa acggcagata
     1081 actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc gaacaactga
     1141 agaaaaacgg acagtatgac aatacaatta ttctctttac ctccgataat ggtgcggtta
     1201 tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag acctatcctg
     1261 gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc ggtaattatg
     1321 acaagctgat ttccgcaatg gatttctacc cgacagctct tgatgcagcc gatatcagca
     1381 ttccaaaaga ccttaagctg gatggcgttt ccttgctgcc ctggttgcaa gataagaaac
     1441 aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg tttgatgagg
     1501 aaaatattcc attctgggat aactaccaca aatttgtccg tcatcagtca gacgattacc
     1561 cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga aataacgatt
     1621 attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaaactg acggatctac
     1681 agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg caaggcgtgg
     1741 taagagagtt tatcgacagc agccagccac cgcttagcga ggtaaatcag gagaagttta
     1801 acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg gcggattttt
     1861 ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc agttttcaat
     1921 gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt actcatgaaa
     1981 aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgca gcgtctggca
     2041 atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg gattttttcc
     2101 gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt aatgcattac
     2161 aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa catgaatttc
     2221 tggttggtat ctcgatcgat ggcccccagg agttacatga ccgttacaga cgcagtaatt
     2281 caggtaacgg tacttttaca aaagtgatag cagccatcga gcgtctgaaa tcatatcaag
     2341 tagagtttaa tacgttaacc gtcattaata acgttaatgt ccattaccct cttgaggttt
     2401 atcatttttt aaaatctatc ggcagtaaac atatgcaatt tatcgaattg ctagaaaccg
     2461 ggacgccgaa tatagatttc agtggtcata gtgagaacac attccgtatc attgattttt
     2521 ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa tgggttaaaa
     2581 acgatgtggg tgaagttttc atccgtcagt ttgaaagctt tgtcagccgt tttttgggga
     2641 atgggcatac cagttgtatt ttccaggagt cctgcaagga taatctggtt gttgaaagta
     2701 atggagacat ttacgaatgc gaccattttg tctatccaca gtacaaaatt ggaaacatta
     2761 ataaatctga actcaaaacg atgaacagtg tacaactgac agcgcaaaaa aaacggattt
     2821 cagcgaaatg tctgaaatgt gcatataaac ctatctgcaa tggcggttgt cctaagcatc
     2881 gtattactaa agtaaacaat gagactgttt cttatttttg cgaaggttat aaaatccttt
     2941 tttcaaccat ggtaccttat atgaacgcca tggtagagtt agctaagaac agagtaccgc
     3001 tttaccacat tatggatgtt gcaaaacaaa tggagaataa ttaataacgc tccctctttg
     3061 ctccagtctt agcctccttc ttatggcctt aacaagacac ccgacataaa ttcctgttgg
     3121 gtgtttcgtc atatcatctt cccttgcgaa aatcctggca tacgtggtat gcgtcagggc
     3181 attagcaata cccttgatta tcaatagcat aaccataacc actgaaaatt aaaaatttct
     3241 atatatttca tattgttaaa tgatacttaa ataaatttca tcagtaaatc attgccatag
     3301 ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat tacgttttgt
     3361 atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa gaataataaa
     3421 acttcagtgt tgttaatcat tattatcctc gccatgatcc ttggcgtggt taaaattcag
     3481 gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa agaaaccgat
     3541 aagctttggc agcttgtcct ttggttccct gccctgctgg ggatctttgt attaatatcc
     3601 gtaaacaaaa catggttaat taaactgctg actattcgct ggcgcgaatg gttaacagat
     3661 tactacctta atcggtggtt tgcagataaa aactactact tcacgcagat ctacggcgag
     3721 cataaaaata cagataaccc tgaccagcgt atcgctgaag acattctctt actgatcagc
     3781 aaaacgctca gtctttcatt tggcttcatc cagtcactta gcatgctgat cacctttacc
     3841 gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac agaatggaat
     3901 atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac tttatttacc
     3961 cataaggtag gtaaacgtat tcgtccgctt aatgtggaaa aacaacgtag cgaagcgaca
     4021 tttcgaacta atcttgtgca gcataataaa caggcagaac tgattgcgct aagcaatgct
     4081 gagtctctac aacgccagga gttgagcgac aattttcata ccattaaaga gaactggcat
     4141 cgtttaatga atcgtcagcg gtggcttgat tactggcaga atatttattc gcgttcgctt
     4201 agcgttcttc cgtactttct gttattgccg cagtttatta gcggacagat caatctgggc
     4261 ggactgatga aatcgcgcca ggcctttatg ctggtatcga acaatttaag ctggtttatt
     4321 tataaatatg acgaacttgc tgaactggct gcggttatcg atcgtttgta tgagttccat
     4381 caactcaccg aacagcgccc tacgaataag cctaaaaatt gccaacatgc ggtacaagtg
     4441 gctgatgcga gtattcgtac tcctgataat aagatcatat tagagaacct gaactttcat
     4501 gtttcgccag gcaaatggct attactgaaa ggttactctg gtgcgggaaa aaccacactg
     4561 cttaaaacat tatcccactg ctggccgtgg tttataggtg atatttcttc tcctgccgac
     4621 agttggtatg tgtcacaaac accgttaatc aaaaccggct tactgaaaga gattatttgt
     4681 aaagcacttc ctctgcccgt agacgataaa tcgttgagcg aaatactgca tcaggttggt
     4741 ctggggaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat tctttccagc
     4801 ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa atggatattt
     4861 cttgacgaaa ctacctctca tcttgaggaa caagaggcta tccgcttact gcgtttagtg
     4921 cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg tgtctggaac
     4981 ctggcagatg atatttgtga cattagcgcg gttttataaa aacaataaaa gccctgctgt
     5041 acggagatat aaataaatga agcgagttct tattcctggc gtcattttat gtggcgctga
     5101 tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat ttttttgaag agatgacggt
     5161 ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg aaagcatcga
     5221 gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc ctgcggtacg
     5281 catggattca acgcaaagta cctcgttgaa ccagggagat attcgcccgg agaaaatctc
     5341 tattcacggt gcgtcgccct accagaatgc ctatttgatt gatggtatta gtgccactaa
     5401 taacctgaac ccagcgaatg agtccgatgc cagtagtgca accaatatta gcgggatgtc
     5461 acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg acagttttgt
     5521 gccggttgaa ttcggtcgct tcaatggcgg ggtaattgat gcaaagatca aacgcttcaa
     5581 cgctgatgat agcagcgtga aactgggtta tcgcactacg cgttcggact ggttaacatc
     5641 gcatatcgat gagaataaca agagcgcatt taatcaaggt tcttcaggaa gtacttatta
     5701 ctctccagat tttaaaaaga acttttatac cttgtcgttt aatcaggaac tcgctgataa
     5761 ctttggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg cggattatgt
     5821 ttcgaatgac ggcattgtcg ccggtcgggc acagtataaa aacgttatcg atactgcatt
     5881 gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct taaaatatac
     5941 cggctccagc cgtgattata ataccagcac cttcccgcag tctgatcgcg aaatgggtaa
     6001 taaatcctat ggtctggcat gggatatgga tacgcagctc gcatgggcca aactacgtac
     6061 caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca tctggtacac
     6121 cgaactttca tgtacatatg gtgatattac agggcgttgc acccgtggcg gattaggaca
     6181 catttcccag gctgtagata attacacctt caaaacacgc ctggactggc aaaaattcgc
     6241 cgtgggtaat gtttcgcatc aaccctactt cggtgcggaa tacatctatt ccgatgcgtg
     6301 gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgccggaa agaaaactaa
     6361 ccataccatt taccataaag gtaaaggcag cctgggaatt gacaactaca cactgtatat
     6421 ggcggatcgc attagctggc gtaatgtgtc attaatgccc ggcgtgcggt atgactatga
     6481 caactatctg tcaaaccaca atatctcccc gcgctttatg acggaatggg atatttttgc
     6541 tgatcaaacc tcaatgatta ccgcaggtta taaccgttac tatggtggga atattcttga
     6601 tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggca ataaaaccct
     6661 gacgcgttat caggatttga aaacgcctta taacgatgaa ctggcaatgg gattgcagca
     6721 gaaaatcggt aagaacgtta ttgcgcgtgc aaactatgtt taccgtgaag cgcatgatca
     6781 aatcagcaaa agcagtcgca ccgacagcgc gactaaaacc accattactg aatataacaa
     6841 cgatggcaaa accaaaacgc attcgttcaa cctcagtttt gagctggccg aacccctgca
     6901 tatccgccag gtagatatta acccacaaat tgtctttagc tatatcaaga gcaagggcaa
     6961 cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccagg tggtttataa
     7021 cggtaatctt gtctcttacg atagcgttcc agtggcagat tttaataacc cattaaagat
     7081 ctccttaaac atggatttca cgcatcaacc gagcgggtta gtgtgggcga atacgctggc
     7141 ctggcaagaa gcgcgtaaag ctcgcattat cctgggtaag gcgaatgcgc aatacatcag
     7201 cgaatattca gattacaagc agtatgttga cgaaaaactg gatagcagcc tgacctggga
     7261 cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga tcagtgccga
     7321 tattctcaat gtactggata gcaaaaccgc ggttgataca acgaataccg gtgtggcgac
     7381 ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat agttcggatt
     7441 ttacttaacc gggtaaattc gcccggtttt cgcatggaga taattatgag aaacctctgt
     7501 ttcttactga cgttagtggc aactctgttg ctccccgggc ggctgattgc cgccgcctta
     7561 ccgcaggatg aaaagttaat taccgggcaa ctggacaatg gcttgcgata tatgatttat
     7621 ccgcatgctc atccaaagga tcaggtaaat ttatggctgc aaattcatac cggttcattg
     7681 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
     7741 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agttaatggg cctgcgtttt
     7801 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgcca
     7861 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
     7921 gcaacctttg aaaaactcga agtagacgct gaacgcggcg taattactga ggaatggcgt
     7981 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
     8041 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacaccg
     8101 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
     8161 gttggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
     8221 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
     8281 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
     8341 ccaatggtac aagtgaacga tgagcaaagc tttatcgaac aagctgaatg gagcatgtta
     8401 gttcagctgt tcaatcaacg tctgcaggaa cgtatacagt cgggcgagtt gaagactatt
     8461 tctggcggca ctgcgcgcag cgttagaatt gcacccgatt atcagtcgct gtttttccgt
     8521 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
     8581 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
     8641 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     8701 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     8761 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     8821 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgcaaaa
     8881 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     8941 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     9001 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     9061 gccagggtta ttctggcaaa atccgcgggt gaagagcaaa aactacaaat tattgccgta
     9121 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     9181 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     9241 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     9301 agcgcgcgga ctaataaccc tgaacctggt tttcagttga ttaaccagcg aatcacccac
     9361 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     9421 acgctcgacc agcgtccagc ggagaaattc gcccagcaga tgtatgagac gcgctatgct
     9481 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     9541 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     9601 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggatc aatcaaacac
     9661 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     9721 actgtaaagg aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     9781 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     9841 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     9901 ctctcggttg atcctcaggc caaagatatc agtcatttgc tggcttttac ttgtcaacca
     9961 gaacgacatg atgaactgtt aacgttagcg aatgaagtga tggttaagcg tctggctaaa
    10021 gggatcagtg agcaagaact gaatgaatac cagcaaaacg ttcagcgcag cctcgatatc
    10081 caacagcgta gcgttcaaca attagcgaac actattgtaa atagtcttat tcaatatgac
    10141 gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacggt agagaatgtt
    10201 aacactgccg ttaaacaata tctttctcat ccggtaaata cttatactgg agtattattg
    10261 ccaaaataat aacagccccg tcaacacatc gttggcgggg attt
//
LOCUS       DAZFFG010000145        10103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFFG010000145 DAZFFG010000000
VERSION     DAZFFG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.145"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(84..159)
                     /locus_tag="ACHJ7J_004284"
     tRNA            complement(84..159)
                     /locus_tag="ACHJ7J_004284"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(124..126),aa:Lys,seq:ttt)
     gene            complement(193..268)
                     /locus_tag="ACHJ7J_004285"
     tRNA            complement(193..268)
                     /locus_tag="ACHJ7J_004285"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Lys,seq:ttt)
     gene            complement(415..490)
                     /locus_tag="ACHJ7J_004286"
     tRNA            complement(415..490)
                     /locus_tag="ACHJ7J_004286"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(455..457),aa:Lys,seq:ttt)
     gene            complement(494..569)
                     /locus_tag="ACHJ7J_004287"
     tRNA            complement(494..569)
                     /locus_tag="ACHJ7J_004287"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(534..536),aa:Val,seq:tac)
     gene            complement(621..696)
                     /locus_tag="ACHJ7J_004288"
     tRNA            complement(621..696)
                     /locus_tag="ACHJ7J_004288"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Lys,seq:ttt)
     gene            complement(699..774)
                     /locus_tag="ACHJ7J_004289"
     tRNA            complement(699..774)
                     /locus_tag="ACHJ7J_004289"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(739..741),aa:Val,seq:tac)
     gene            complement(810..885)
                     /locus_tag="ACHJ7J_004290"
     tRNA            complement(810..885)
                     /locus_tag="ACHJ7J_004290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(850..852),aa:Lys,seq:ttt)
     gene            complement(1050..1841)
                     /gene="cpoB"
                     /locus_tag="ACHJ7J_004291"
     CDS             complement(1050..1841)
                     /gene="cpoB"
                     /locus_tag="ACHJ7J_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO5002444.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1851..2372)
                     /gene="pal"
                     /locus_tag="ACHJ7J_004292"
     CDS             complement(1851..2372)
                     /gene="pal"
                     /locus_tag="ACHJ7J_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO5002445.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2407..3699)
                     /gene="tolB"
                     /locus_tag="ACHJ7J_004293"
     CDS             complement(2407..3699)
                     /gene="tolB"
                     /locus_tag="ACHJ7J_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO5002446.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3832..5097)
                     /gene="tolA"
                     /locus_tag="ACHJ7J_004294"
     CDS             complement(3832..5097)
                     /gene="tolA"
                     /locus_tag="ACHJ7J_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5002447.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEDAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(5162..5590)
                     /gene="tolR"
                     /locus_tag="ACHJ7J_004295"
     CDS             complement(5162..5590)
                     /gene="tolR"
                     /locus_tag="ACHJ7J_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO5002448.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5594..6286)
                     /gene="tolQ"
                     /locus_tag="ACHJ7J_004296"
     CDS             complement(5594..6286)
                     /gene="tolQ"
                     /locus_tag="ACHJ7J_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO5002449.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6283..6687)
                     /gene="ybgC"
                     /locus_tag="ACHJ7J_004297"
     CDS             complement(6283..6687)
                     /gene="ybgC"
                     /locus_tag="ACHJ7J_004297"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5002450.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6837..7130)
                     /gene="ybgE"
                     /locus_tag="ACHJ7J_004298"
     CDS             complement(6837..7130)
                     /gene="ybgE"
                     /locus_tag="ACHJ7J_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO5002451.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADMVLIVGLIFFFF"
     gene            complement(7130..7243)
                     /gene="cydX"
                     /locus_tag="ACHJ7J_004299"
     CDS             complement(7130..7243)
                     /gene="cydX"
                     /locus_tag="ACHJ7J_004299"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO5002452.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7258..8397)
                     /gene="cydB"
                     /locus_tag="ACHJ7J_004300"
     CDS             complement(7258..8397)
                     /gene="cydB"
                     /locus_tag="ACHJ7J_004300"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5002453.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8413..9981)
                     /gene="cydA"
                     /locus_tag="ACHJ7J_004301"
     CDS             complement(8413..9981)
                     /gene="cydA"
                     /locus_tag="ACHJ7J_004301"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO5002454.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2450 a         2756 c         2441 g         2456 t
ORIGIN      
        1 gcgctcaact cgaacctgct gcaggttcga atctttgagt atcgcagata gcaaaaaagc
       61 gcctttaggg cgctttttta cattggtggg tcgtgcagga ttcgaacctg cgaccaattg
      121 attaaaagtc aactgctcta ccaactgagc taacgacccc ttgcgggatt atgctgcttt
      181 aatcattcag gatggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
      241 actgctctac caactgagct aacgacccat acgggtgtta cctgaaagat tgttactcgg
      301 ggccaatctt taagattggt gggtcgtgca ggatgactcg cttcgctcgc cctacgggcc
      361 gttgctggcg caacgttatc cttcacgctt aacatctgag tttgatgtta aaactggtgg
      421 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
      481 ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg
      541 aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca aactacttcc
      601 accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
      661 aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga
      721 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgatact
      781 acgctggata ctacaactaa ctaggttagt ggtgggtcgt gcaggattcg aacctgcgac
      841 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
      901 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
      961 caacaaaaat ttcgatgtag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
     1021 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgcgcctgt
     1081 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
     1141 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc tgcagccttt
     1201 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
     1261 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
     1321 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
     1381 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat caccgctttt caccggcgcg
     1441 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgccgc accgctttga
     1501 tcaccgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
     1561 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
     1621 cgcagggaat caatatcgga ttgattatca gagagttgtt gctggagttg ggttaaaagc
     1681 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
     1741 ccgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
     1801 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
     1861 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
     1921 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
     1981 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
     2041 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
     2101 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
     2161 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
     2221 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
     2281 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
     2341 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
     2401 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
     2461 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
     2521 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
     2581 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
     2641 accgttggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
     2701 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
     2761 ctgtggacga ccggcctggt cagaagtaaa tgccaggttc tggctgtccg ggaaccaggt
     2821 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
     2881 cattacgtac aggttcaggc taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
     2941 tggcgagaat gcaggcgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
     3001 gtttgccagc gtttggataa ccagtgcgga acgaccgctt tcgaaggtca cataagccag
     3061 tttagaaccg tctggtgacc acgccgggga catcagcggc tgtggtgaac ggtgaacgac
     3121 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
     3181 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaataccgg tcagtttttc
     3241 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
     3301 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
     3361 attgtaagaa ccatccggat tcggagtgac ctgaccgaca actacagcgt caatacccag
     3421 cgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
     3481 gcgatctaac ggattaaatt taccgctgtt acgcaagtcg gcagcaacga tgccgccaat
     3541 atcttcaggt gccgcacctg ggcccgccca ctggaaagga acaactccaa taggacgacc
     3601 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
     3661 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
     3721 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
     3781 cccgtggcaa ccggtgcctg atattgaccg tcggaacagt caacatcgcg attacggttt
     3841 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
     3901 cttcgcaagt ttagctgctg ccaacgcagc ttgacaaagt gcgggatcgc caccttcagg
     3961 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
     4021 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
     4081 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
     4141 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
     4201 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
     4261 ttttgcagct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
     4321 tgctgccttt tctgcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
     4381 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
     4441 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
     4501 cgcttccgct ttcttcttca gcgccgcagc ggctgcctcg gcttttttct gcgcttcggc
     4561 tgcggctttg gcggcttctg cttctgcttt tttcttcgca tctgcagccg ctttcttcgc
     4621 tgcatcttcc gcagctttag catccgcttc ggccttcgct ttagcatctg ccgccgcttt
     4681 cgctgccgcc tcttccgctt gcttctgctt taactcggcc tgttttgcgg cttcttcagc
     4741 ctgctttttc tgttcctgag cagctaaccg ctctttctca agttgcttca ggcgttcctg
     4801 ttcagccgct tgtttctcac gcagttcttc agcagcctgc tgttccttca tcttgcgctg
     4861 ctcatcagaa cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact gctcaactac
     4921 cgcacctgaa tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc
     4981 ttctatattc tcatcgaacg aactccagat cagcgccgca aataagatga catgcagcac
     5041 tgctgaaata attatcgccc gcttgagctt gtcgttttgt tcggttgcct ttgacactct
     5101 cggtttccaa aaactgttcg cctgttaccc gctctctttc aagcaaggga aacgcagatg
     5161 tttatatagg ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt aacaagttca
     5221 gtgctttaat tatttcatcg taaggcacat cttttgcgcc cccgatcaga aagaccgttt
     5281 tcgggttggc cttgaaacgg ctggacactt ccgccaccac ttgctctggt ggtaaacgct
     5341 ccaggcgatc tttctcaacc accacggtgt actgaccaat accagacact tcaacaatca
     5401 ctggcggatt atcgttactg ctcaccgcct gtgattcagt agcgtctggc agatcgacct
     5461 ccacgctctg ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc accagcagta
     5521 cgtccagcaa cggtacaatg ttgatttcgg acttgagatc gcgacgacct cgtccacgcg
     5581 ctctggccat ggcttacccc ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa
     5641 tcgcggtaaa ctcttccata aagttgtcgt aattcagttc cagtttgttt acgcgctggt
     5701 tgaggcggtt gtaggccata actgccggaa ttgcggcgaa cagaccaatt gccgtcgcaa
     5761 tcaacgcttc tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt accgccccga
     5821 gggcgataaa ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata tacgggctga
     5881 tggagccaac cgtaccgagg aacggaatgt gcgtttccag attttcaagt tcacggttca
     5941 tggaaatacg catcgcacgc gacgcccctt ctactacggc ttccggcgca tggctattgg
     6001 cacgatgcag gcgcacaaat tctttgaacc cgctgtaaaa gatttgttcc gaaccggtca
     6061 gattatcccg tttcccctgg ctctcttgat agaggcgaga gagttcgatt ccagaccaga
     6121 atttatcctc aaacgcttcg gcttcgcgcg ccgctgcgtt aagaatacgg gtccgctgga
     6181 taataatggc ccaggatgcg attgaaaaac caatcaaaat caacatgata agtttaacca
     6241 gaaggctagc cttcaggaac aaatcaagga tattcatgtc agtcactgct taaactccgc
     6301 gacaatagac ttgggaagcg cacgaggctt cattttgagt gggtcaacgc aaacaaccag
     6361 aacctctgct tcattcagca aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa
     6421 agaggtgcca cgcattgatg ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc
     6481 aggtgcgtaa tattccaccg tcattttacg taccacaaag gcaacgcgtt cagccatcag
     6541 cgcctgctga ctgaagtgat gatgacgcag catctctgtg cgtgctcttt cataaaaagc
     6601 gacgtaactg gcgtggtaca ccacaccacc agcatcggta tcttcatagt agacgcgaac
     6661 cggccatcga aacagcgttg tattcacttt acatcctggt aatgcaacaa aagttagagc
     6721 ttttaaacgc tgctactata cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg
     6781 agcgtaaaaa tttatgggcc tggcgcggcc cataattttt taaagagaat tctgatttaa
     6841 aagaagaaga aaatcagccc gacaatgaga accatatcgg caagcaatgg gcaaaaaatc
     6901 ccttgccaaa gaaccttctg cggacgaaag cccacgccgt gaatcacacc agcacagacg
     6961 gcccacatca gcaataaacc atgccagatt tccagttcac tggtcttcgc ggcgaaacgt
     7021 gatgggtccc aaaacataca tcctgctaac agaagcgcca tcacgaagga aagcgcccgc
     7081 agggggcgct tgtccattac cgcatacaag gttgcgataa tcttactcat cagatgtctt
     7141 cttgaccggc tttgcctgat tcgacgtgct caagcgccag cgcggtgatt accccaaacg
     7201 aacaggcaag aagcgttccc agaatccatg cgaaatacca catttttagc tccttactta
     7261 gtacagagag tgggtgttac gttcaatatc ttctttggtg atacgaccga acattttcca
     7321 gtaacaccag gcggtgtaga gcagaatgat cggtaccaga accaccgcaa cccaggtcat
     7381 gacgttaagc gtcagctggc tggaagttgc atcccacatt gtcagacttg cgttcatcat
     7441 ggtgctggac ggcatcacaa acgggaacat tgcgataccg gcagtcagga tgatgcaggc
     7501 cagcgtcagg gaggagaaca caaacgccca tgcggcttta tccatacgtg cagtcaggat
     7561 ggtcagcagc ggcagaacca cacccagtgc cggaatagcc cacagaattg gcgtgttgtt
     7621 gaagttaacc agccatgcgc cagcttcacg aaccacttct ttattcagcg ggttagaggc
     7681 tgcgtaatgg tccattgtcg atttcacgac ataaccatcg ataccgtaca tcacccatac
     7741 accagccagt gcgaagcaga ccagtgtcac cagtgccgcc acctgagccg ttgcacgggt
     7801 acgcaggtgc agttcgccca cggtacgcat ttgcagatag gttgcgccct gagtaatgat
     7861 catccctacg ctcaccacgc ctgccagcag gccgaacggg ttaagcagct ggaagaagtt
     7921 accggtgtag tacagacgca gatattcatc aacgttgaac ggtacgccct gcaacaggtt
     7981 accgaacgct acaccaatca ccaacggcgg aacgaagcta ccaatgaaga tgccccagtc
     8041 ccacatgtta cgccagcggg tttcttcaat cttggagcgg tagtcaaaac cgaccggacg
     8101 gaagaacaaa gacgccagca cgaggatcat cgccacatag aagccggaga acgcagcggc
     8161 atagaccatc ggccaggcag caaacagtgc gccgcctgcg gtgatcagcc aaacctggtt
     8221 accgtcccag tgtggtgcga tggagttaat cataattcga cgctcggtgt cgttacgacc
     8281 gaggaaacgg gtgagcatgc ccacccccat gtcgaaacca tcagtgactg caaaaccaat
     8341 cagcagaacg ccaaccagca gccaccagat aaaacgcaat acttcataat cgatcatttg
     8401 acgactcctg tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa agtgatagcg
     8461 accggttttc aggctgcttg ggccgaggcg tgcaaacttg aacattaaga acaattctgc
     8521 caccaggaac agggtataca ggccgcaaat cagcaccatt gagaagatga gatcgcctgc
     8581 ggtcagtgac gagttcgcca cagctgtcgg cagcacttca ccgatagccc acggttggcg
     8641 gccatattca gccacgaacc agcccgcttc tacagcaatc cacggcagcg gaataccgta
     8701 cagcgcggcg cgcagcagcc attttttctc gccaatgcgg ttgcggatga cactccagaa
     8761 agagagcgcg atgattgcca gcagcaggaa gccacacgcc accatgatac ggaacgcgaa
     8821 gtacagcggc gctacacgcg gaatggagtc tttggttgcc tgttgaatct gcgcttcagt
     8881 cgcatcagcc acgtttggcg tatagcgttt cagcagcaga ccgtaaccga ggtccttctt
     8941 catgctattg aactggtcac gaaccgcctg gtcggtagaa ccagaacgca gttgttcgag
     9001 cagagagtac gccttcatcc cgttacgaat gcgttcttca tgctgcacca tcagttcttt
     9061 caggccgata actggagtat ccacggaacg cgttgcaatg atgcccagcg cgtaagggat
     9121 ctggatcgca aatttgttcg tctcctcttc ctgatcagga atgccgaaca gagtaaaggc
     9181 agcaggcgca ggttgcgttt cccactcagc ttcaatagca gccagtttgg ttttctgcac
     9241 gtcgcccatt tcgtagccgg attcatcacc cagaacaata acagacagaa cggcagccat
     9301 accgaagctg gcagcgatag caaaggagcg tttagcgaag gcgaagtcac gacctttcag
     9361 catataccat gcgctgatac cgaggatgaa catcgcgcca gtcacataac cagacgctac
     9421 agtgtgaacg aatttcacct gagcaaccgg gttaagcacc agctcggaga agctcaccat
     9481 ctccatacgc atagtttcaa agttgaaatc ggacgcgatt gggttttgca tccagccgtt
     9541 cgcaaccaga atccacagtg cggacaggtt tgaaccgagc gccaccagcc aggtgacaca
     9601 catatgctga actttaccca gacgatccca accgaagaag aacagaccta caaaggtgga
     9661 ttcgaggaag aaggccatta gaccttcaat tgccagcggc gcaccgaaga tatcccctac
     9721 atagtgggaa tagtaagacc agttagtccc gaactggaac tccatggtca gaccggtagc
     9781 cacacccaga gcgaagttga taccaaacaa cttgccccag aacttggtca tatctttata
     9841 aatctgtttg ccggaaagga cgtagaccgt ttccataatg gccagcagga acgccatacc
     9901 gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact gtaagcgcga
     9961 cagttcgact atatctaaca tcatgactcc ttgctcatcg catgaagact ccgagagtga
    10021 accccgttag aaagggtcac acgcatgccc caatacaaaa ttatttgctc gcctctatcg
    10081 ttgccatttc atcaaaagag gaa
//
LOCUS       DAZFFG010000146        10082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFFG010000146 DAZFFG010000000
VERSION     DAZFFG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.146"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            151..654
                     /gene="ftnB"
                     /locus_tag="ACHJ7J_004302"
     CDS             151..654
                     /gene="ftnB"
                     /locus_tag="ACHJ7J_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO5002455.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(733..984)
                     /gene="yecJ"
                     /locus_tag="ACHJ7J_004303"
     CDS             complement(733..984)
                     /gene="yecJ"
                     /locus_tag="ACHJ7J_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO5002456.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1100..1186)
                     /gene="azuC"
                     /locus_tag="ACHJ7J_004304"
     CDS             complement(1100..1186)
                     /gene="azuC"
                     /locus_tag="ACHJ7J_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO5002457.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1449..1772
                     /gene="yecR"
                     /locus_tag="ACHJ7J_004305"
     CDS             1449..1772
                     /gene="yecR"
                     /locus_tag="ACHJ7J_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO5002458.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQSHHSA
                     FPVFL"
     gene            1943..2440
                     /gene="ftnA"
                     /locus_tag="ACHJ7J_004306"
     CDS             1943..2440
                     /gene="ftnA"
                     /locus_tag="ACHJ7J_004306"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO5002459.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2478..2717)
                     /gene="yecH"
                     /locus_tag="ACHJ7J_004307"
     CDS             complement(2478..2717)
                     /gene="yecH"
                     /locus_tag="ACHJ7J_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO5002460.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            2908..4119
                     /gene="tyrP"
                     /locus_tag="ACHJ7J_004308"
     CDS             2908..4119
                     /gene="tyrP"
                     /locus_tag="ACHJ7J_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO5002461.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRRHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4181..4846)
                     /gene="yecA"
                     /locus_tag="ACHJ7J_004309"
     CDS             complement(4181..4846)
                     /gene="yecA"
                     /locus_tag="ACHJ7J_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO5002462.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5042..5128)
                     /locus_tag="ACHJ7J_004310"
     tRNA            complement(5042..5128)
                     /locus_tag="ACHJ7J_004310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5092..5094),aa:Leu,seq:taa)
     gene            complement(5141..5214)
                     /locus_tag="ACHJ7J_004311"
     tRNA            complement(5141..5214)
                     /locus_tag="ACHJ7J_004311"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5180..5182),aa:Cys,seq:gca)
     gene            complement(5269..5344)
                     /locus_tag="ACHJ7J_004312"
     tRNA            complement(5269..5344)
                     /locus_tag="ACHJ7J_004312"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5309..5311),aa:Gly,seq:gcc)
     gene            complement(5496..6044)
                     /gene="pgsA"
                     /locus_tag="ACHJ7J_004313"
     CDS             complement(5496..6044)
                     /gene="pgsA"
                     /locus_tag="ACHJ7J_004313"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO5002463.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6101..7933)
                     /gene="uvrC"
                     /locus_tag="ACHJ7J_004314"
     CDS             complement(6101..7933)
                     /gene="uvrC"
                     /locus_tag="ACHJ7J_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO5002464.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     HNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7930..8586)
                     /gene="uvrY"
                     /locus_tag="ACHJ7J_004315"
     CDS             complement(7930..8586)
                     /gene="uvrY"
                     /locus_tag="ACHJ7J_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO5002465.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8728..8799
                     /locus_tag="ACHJ7J_004316"
                     /pseudo
     CDS             8728..8799
                     /locus_tag="ACHJ7J_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8882..9058
                     /gene="yecU"
                     /locus_tag="ACHJ7J_004317"
     CDS             8882..9058
                     /gene="yecU"
                     /locus_tag="ACHJ7J_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO5002466.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9045..9269
                     /gene="yecF"
                     /locus_tag="ACHJ7J_004318"
     CDS             9045..9269
                     /gene="yecF"
                     /locus_tag="ACHJ7J_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO5002467.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9337..10059)
                     /gene="sdiA"
                     /locus_tag="ACHJ7J_004319"
     CDS             complement(9337..10059)
                     /gene="sdiA"
                     /locus_tag="ACHJ7J_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO5002468.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2532 a         2537 c         2331 g         2682 t
ORIGIN      
        1 gttcagacgt tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg
       61 accggcagaa aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc
      121 tctccacttc tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc
      181 aactctcaaa tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg
      241 tgttctgaac agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat
      301 gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt
      361 aaagccattg atgttcccgg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg
      421 atggaagaat acgagcaacg ttctagtacg ttggcacagt tagccgatga agcgaaagaa
      481 ctgaatgatg attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat
      541 ggcctgttgc tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc
      601 ccagtgcaga ccgaccaaca tgttctgaat gtcgtgtcgc atcagctgca ttgatcatac
      661 atcggcgcta atgcattgcg ccgatgaagt ttttgagaaa ccgctgcctc atctttttga
      721 agcagcggtt ttttaatggg attcgccttg tggggtaaac ttgagttcaa tcagcgcgat
      781 ggctttttgg attgcccgca tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc
      841 gatattcttc agctggctgg acattttttc gcgaacttca acgggcgcga ttacatcgag
      901 aacatccaga atttgtttga taaccaactg gcaagcaacc acatcagaaa ccagttcctg
      961 atcggcattc agcggctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag
     1021 tagcggcttc acatcttcag cgatagtcac atccaccgtc accaggacac aaaaaaacct
     1081 gccggagcag gtttttttgt tatcggaaca tattgcctgg cggtacgtct ttgaacgtct
     1141 tgcaatagtt attgaacata cttttcagga ttttgcgcag tttcatcgcg gcactccgac
     1201 catttgttat acaggtgtta ttgtctttgc gcttataata tgacaaccat cacaaaaatc
     1261 aatctttatg tgatacaaat cacataaata cccctttaat gttataaaaa tgataatcaa
     1321 aaaacagccc ccctatttct gacacctaca gatggcgaga aatagcgcct gccaggcgtc
     1381 ttttccggcc attgtcgcag cgctgtaacg cgtaaaatag tgctttctct tactcttctg
     1441 gctggaccat gagacttctg attctgactc tttcattaat aacgctggcg gggtgtacgg
     1501 ttactcgcca ggcccatgtg agcgaagttg acgcggcaac aggtattgta cggttggttt
     1561 atgaccaggc ttttttgcag catgcccata ccgatcgtta tgtcagtcgc gggatcgccg
     1621 atcgcgcatg ccagcaggaa ggctataccc acgcgatccc ctttggtcag ccagtaggca
     1681 actgtagcct ttttgcgggt tccttatgtt tgaatacgga attcactttg tcatatcaga
     1741 gccatcattc cgccttccct gtctttctct aaatatttta atcagcgagg ggatcttcgc
     1801 tgattaaaga aatagcggta atgcgtttta ttcccattcg tatttttaat aattgaagtt
     1861 tatattttac cttttgcaaa taataaaata acaaattata gtgacgccac agcaacaaat
     1921 ataacctttg tggagcacta tcatgctgaa accagaaatg attgaaaaac ttaatgagca
     1981 gatgaacctg gaactgtact cttcactgct ttatcagcaa atgagcgcct ggtgcagcta
     2041 tcataccttc gaaggtgctg ccgcgttcct gcgccgccac gcccaggaag agatgacgca
     2101 tatgcagcgt ctgtttgatt acctgactga taccggaaac ttaccgcgta ttaataccgt
     2161 tgaatctccg tttgctgaat attcttcact tgatgaatta ttccaggaaa cctataaaca
     2221 cgaacaatta atcacgcaga aaattaacga actggctcat gctgcaatga ccaatcagga
     2281 ctacccaaca tttaatttcc tgcaatggta tgtttctgag cagcatgaag aagagaaact
     2341 gttcaaatcg attattgata aattaagcct ggcaggcaaa agcggcgaag gtctgtattt
     2401 tatcgacaaa gaactctcta ccctcgacac acaaaactaa tgctctccgg cggcaggttt
     2461 tctgccgccg tttcaaatca gtgacggcaa atcttactct gatcggtcga aaaaccgtct
     2521 tcagaaggaa taaatttgcc ttttgctgcc agaaacgcca ccagctctcc cgctgtcatc
     2581 ccttctgccg agcaggtgtg aaaacgtgcc tgttcaccaa aacgcgcttt aatcgcagct
     2641 tccagactgg catgcgtata ttgctcgcct gattcaatca tcatatttaa cacttcatga
     2701 ccgtgtatag agtccatcgt tcctcctcaa aaaaaggcta gcgtagcgat taccgcttat
     2761 gaagactttg cgccagcgca ggactgaatg ctttttattg tacatttata tttacaccat
     2821 atgtaacgtc ggtttgacga agcagccgtt atgccttaac ctgcgccgca gatatcactc
     2881 ataaagatcg tcaggacaga agaaagcgtg aaaaacagaa ctctgggaag tgtttttatc
     2941 gtggcgggaa ccacaattgg cgcaggtatg ctggcaatgc cgctggctgc ggcaggtgtt
     3001 ggttttagcg tcacgttaat cttgttgatt gggctttggg cgttgatgtg ctacacggcg
     3061 ctattactgc tggaggtgta ccagcatgtt ccggcagata ccggtcttgg cacgctggca
     3121 aaacgctatc tgggacgcta cggtcagtgg ctgacgggct tcagtatgat gttcttaatg
     3181 tatgctctga ctgcggcata catcagcggt gccggtgaat tgttggcctc cagcatcagc
     3241 gactggacag gtatttcaat gtcggcaacc gctggcgtgc tgttgttcac ttttgttgcc
     3301 ggtggcgtgg tttgtgttgg aacatcgctg gtcgatttat ttaaccgttt tctgttcagc
     3361 gccaaaatta tctttctggt ggtgatgctg gtattgctgc taccgcatat tcacaaagtg
     3421 aatcttttaa ccctgccgtt gcaacagggg ctggcactgt ctgcaatccc ggtgattttt
     3481 acgtcgtttg gttttcacgg tagcgtgccg agtattgtca gctatatgga tggcaacatt
     3541 cgtaagctac gctgggtgtt tataaccggt agtgcgatcc ccctggtggc atatattttc
     3601 tggcaggtgg cgacgcttgg cagcattgat tcaacaacct ttatgggatt gctggctaat
     3661 catgccggat taaacgggct gctacaggcg ttacgcgaaa tggtggcctc tccgcatgtt
     3721 gagctggcag tgcatttatt tgctgattta gccctcgcca catcatttct cggcgttgcg
     3781 ttaggcttat ttgattatct ggctgatttg tttcagcgtt caaataccgt tggtggacga
     3841 ttgcaaactg gagcaattac ctttctgccg ccgttggcgt ttgcactgtt ttatccacga
     3901 ggatttgtta tggcgctggg ttacgccggt gtagcgctgg cggtactggc attgattatc
     3961 ccttcgctgt tgacctggca aagcagaagg cacaatcctc aagcgggtta ccgggtcaaa
     4021 ggtggtcgtc cggcgctggt ggtggtgttt ctctgtggta ttgctgtgat tggcgtgcaa
     4081 tttttgattg cggcagggtt gttaccagaa gtggggtgat tagatagcct caaattcctt
     4141 attgggtgcc agaattaacg ctgacaccca atttgacttc ttaatgcagg cagcactgct
     4201 taaatttctt accactaccg cacgggcaag gatcgttacg ccccggtttc tcttctgctt
     4261 tgatcggttg ctgaacagct ttttcctgcg gatgcgccat ccagtacgca tgtagatcaa
     4321 gcgccgccag tcgaatggca tctacgctct cttcaaacgc ttctggcgac atcttctcta
     4381 cccgctcgaa gttttcctca gtaccgtgca gcgcaatcgc ctccagcgct ggttttaacg
     4441 aatcgggcaa tgttgaccag tcagaaagtg ccacgccccg catatagcca aagcaccact
     4501 cctcaacaat cgtcagctcg ctgccatcaa cttctcgcaa gccgaataac ggctcaaact
     4561 gctccgggaa ttcgttcaga cgctctgcgg tatcggccat atgttgaaaa gccaggttca
     4621 taaagcgcgt catctctttc tctgacgccc agcgcggcac atagtcagcc ccaccccaca
     4681 cggcaaccag ccactgtgcc ggttcaatct cttgcggaga actcaacacc gccgtcaata
     4741 atccgtccag ctccgccaca tcaaggatgg cgtggtcagt gttgtatttg gtcagaatat
     4801 cgtccagcca ttccaactca ctttcgttta acggtcccgt tttcatacgc ttttccttgt
     4861 ggatctcaac tcgccagcac ctatcttaca tgccggtccg tatcagagat actttttgag
     4921 tggctttgcc ggtgattaaa aatcaaggag ggtgtaacga caagttgcag gcacaaaaaa
     4981 accacccgaa ggtggtttca cgacactgct tattgctttg attttattct tatctttccc
     5041 atggtacccg gagcgggact tgaacccgca cagcgcgaac gccgagggat tttaaatccc
     5101 ttgtgtctac cgattccacc atccgggctc gggaagaaag tggaggcgcg ttccggagtc
     5161 gaaccggact agacggattt gcaatccgct acataaccgc tttgttaacg cgccaaattc
     5221 ttcaggcctt tcagccagac atccgcttga ggccgatgtc ttttaaactg gagcgggaaa
     5281 cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac tgagctattc
     5341 ccgcattcat caagcaatca gttaatcact tgattttatt atcgtctggc aatcagtgcc
     5401 gccgttcgat gcgttgcatt ctacttacct ggcgcgatga gtcaacgata tttttcacca
     5461 cttttgatcg tttgctgaaa attgcgccga aacgatcact gatcaagcaa atctgcacgc
     5521 gcagcgctca aatattgcaa cattgaccac agagtcagta ccgcagccac aaagaaaagt
     5581 gcaataccgg cgtactcaac ccaaatgttc ggacgccaca gcagccatgc caacgccacc
     5641 atctgggcag tggttttcac tttcccaatc caggagacag ccacgctact gcgtttaccc
     5701 aactccgcca tccattcgcg tagcgcagaa ataataattt cacgggcgat catcgttgcc
     5761 gccggtaagg tcacccacca gctgtggtaa tgctcggtta ccagcaccat ggcgatagcc
     5821 acgagaactt tatctgctac agggtcaagg aaagcaccaa accgggtact ctggttccag
     5881 cggcgtgcca gaaaaccatc gaaccagtca gtcaccgccg cgacgcagaa aatgagcgcg
     5941 gcggcaaacg gcgaccaggt gacaggcaga taaaagacca atacaaagaa tgggataagg
     6001 atgacacgga acagtgtaag caacgtaggg atattaaatt gcataatgac gggtaactat
     6061 ctgttgtcag taagattacc cctatgttgc tacagagaca tcaatgtttc aacgaccaga
     6121 agatcttttc tgccagacct tgcgaaatac ccggcacttt tgcaatttcc tcgacgctgg
     6181 cgttacgtaa accttgcaaa ccgcccatat atttcaacaa catttgccga cgttttggcc
     6241 cgacgccttc aatggtttcc agggaactgg tatttttgac cttcgcccgt tttttacggt
     6301 gcccgccaat cgcgtgatcg tgtgattcat cgcgaatatg ctggataaca tgcagcgcgg
     6361 gagaatctgg cggcaaactg aatccctcac cttccggctc aaagaacagc gtttccagcc
     6421 cagccttacg atctgctcct ttggcaacgc caagtagcag cggatgattt ttatcccatg
     6481 agacatccag ttcggcgaag acatttttcg cctgcgcaag ctggcctttg ccgccgtcga
     6541 taagtatcac atccgggatc ttactgtcgt cgatggcttt accataacgc cgacgcagca
     6601 cctgattcat cgccgcataa tcatcgcccg gcgtgatgcc agtaatgtta tagcgccgat
     6661 actccgcacg cagcgggccg ttagcatcaa acaccacaca ggaagcgacg gtttgttcac
     6721 ccatggtatg gctgatgtca aagcactcca tccgcttcac ttccggcaat ttcaacacgc
     6781 tggcaagtgc tgtaagccgc tggtgaacgg tagattgctg cgaaagtttg ctggttaagg
     6841 ccgtcgccgc attggtgcgc gcgagtttca ggtaacgcgc cctatcgccg cgaggtttgg
     6901 tttgaacatt aatcttgcgt cccgccagtt ctgaaaggga atcggcgagc agcgttttat
     6961 cgctaagatt aaaatcgagc aggatctcac ccggtaaggt gcgcatctgg ctgccttgta
     7021 aatagaactg tcctacgaag gtttccacca cctcgctcag ttccgtaccg ccaggcactt
     7081 tcgggaaata gctgcggctg ccaagcactt tgccctgacg aatgaacaat acgtggacac
     7141 aagccatgcc cgcatcgaac gccacaccaa taacgtcaag gtcgtcgccg gtattggaaa
     7201 caaactgttt ttcggtgacg cgtcgcaccg cctgaatttg gtcgcgaata cgtgccgctt
     7261 cttcaaactc cagattgtga ctggcagttt ccatacggct aatcagttgc gtaagcacct
     7321 gatcatcttt gccagacaaa aacaggcgca catactcgac ctgctgagcg tattcttctt
     7381 cactaaccag tccttcaacg cacggtccca gacagcgccc tatctgatat tgcagacatg
     7441 gacgcgagcg attgcgataa acactatttt cgcactggcg aatggggaaa atcttttgca
     7501 gtagcgccag tgtttcacgt acggcatagc cattcgggaa cgggccgaaa tattcacctt
     7561 tggcatgctt cgcaccacga tgcatcgcca gacgcgggtg ggtatcgcca ctcaggaaga
     7621 taaaaggata tgatttatca tcgcgtagca aaacattgta acgcggctga tagagtttga
     7681 tgtagttgtg ttccagcaac agcgcttcgg tttctgtatg agtaaccgtt acatcaattt
     7741 gctggatctg ggcgaccagc gcttcggttt tgcgcgaagc gaggttgcta cggaaatagc
     7801 tggaaagccg ttttttcagg tctttcgctt tgccgacata gataaccgta ccaccagcat
     7861 cgtacatgcg ataaacgcca ggctggctgg ttacggtttt taaaaacgct tttgcgtcaa
     7921 actgatcact cactgacttg ataatgtctc cgcattacac agaccatggc gaattgccag
     7981 gtgagtcagc tcaacatcgc catgaatgtt tagtttactg aacatacgat agcggtagct
     8041 gttcaccgtt ttcggactga gattgagctg ttctgagatc tcattgacct tctggccctt
     8101 ggtgatcatc agcataatct gcaattcacg ttcagacaaa ctggcaaatg ggctttctgt
     8161 tttttctggt tcgatctggc ttaacgccat ttgttgagcg atgtcagaag caatgtaacg
     8221 ctgccctgaa tagacagaac ggatcgcact cacgacttcc tgcggagccg cgcctttgct
     8281 gaggtagccc gcagcaccgg cctgcatgac tttcgctggt aaagggtttt ctgtatggac
     8341 ggtaagcatg atgattttta catcagctgt ggaacgcgcg attttacgcg tcgcctcaag
     8401 accgccaatg cccggcatac tcatgtccat tagcaccacg tcaacggcat ttgcccggca
     8461 ccacttaacg gcgtcttcac cgcacgatgc ctcaccgacg acttttatac cctttatatc
     8521 ttccagaatg cgtcgtatcc ctgcgcgcac cagttcgtgg tcatcaacaa gtagaacgtt
     8581 gatcaaagga atatctccag aaatagggat aacgctactg atagttagtc attcgtatat
     8641 tagcggtttt tattgcaact ttgaaacgtt aaaaatggtg ggtattcgat ttttctctcg
     8701 tttttgtcaa ttcgattgtc cacagttatg gtcacgcccg tcaccaacgg cttaccggat
     8761 cgtttcacag taagccctgc gacgaaatgt gacaaaaatt gactttattc agcaaaaatg
     8821 aaaatcagcc cgctgaatat gtaacattaa ttaacccgaa caatacgggc aaaaaacatt
     8881 catgataaaa atatttattg gtcattatat taacgtattt tatagcactg ccgatatcac
     8941 gctcaaaaaa caaccactgc tatttttagc aaagcttatg gtatactccg ccgccttaac
     9001 atttttcacc gcaaattttc attgcaacat gacgaggaaa ataaatgagt acgcctgatt
     9061 tttctactgc cgagaataat caagaactgg caaatgaagt ctcctgcctg aaagcgatgc
     9121 tgacgctgat gctacaggcg atgggacaag ctgatgcggg ccgcgtgatg ttaaagatgg
     9181 aaaaacagct tgcgctgatc gaagacgaaa cccaggctgc agtattttcc aaaacggtta
     9241 agcaaattaa acaagcctac cgtcagtaat ataaaaccgg ctgatagcgt acctttcagc
     9301 cggtttttgc atctggcacg caggacagaa aagagatcaa attaagccag tagcggccgc
     9361 gtaacaggca acctgggtct tatttggtgc attaattttt ttctgcatgt ttttctggtg
     9421 gaaattgacc gtattctcag agattgacaa aatcatcgct atctctgctg atgtcttccc
     9481 ttccgccgtc cacctcagaa tttctttttc gcgcttgctg aaattcatct ctggcgtcat
     9541 cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca gtaactgcat
     9601 ttttaattgc agttcatcac taagaatggg tatttcgcgc gcgctgcaac gggaaaagga
     9661 caaaaagccc aacgcccggt ttggcagcat taaatactga gtgacaccgc ggcgtaaacc
     9721 atgtgcgcgc gcggcttccc ataacggctg tgcttcgttg aataagtcat cattccacat
     9781 tagatggccc tgactaaagt tttcagggtt cagcaccgga tcaattgcga gaaagttttt
     9841 tgcctgataa taactaaccc acgcctcagg gtaattggtg tagaaagcca ctttaggtcg
     9901 agtgaatggt accgggtggc ggacacataa cgaatagtaa tcgtactcca gctgctgagc
     9961 ctgaagctca atttcatggt agacctcttc tgcggtctcc atcctctgaa aacgcaacag
    10021 catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca acgcccctga
    10081 ga
//
LOCUS       DAZFFG010000147         9968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFFG010000147 DAZFFG010000000
VERSION     DAZFFG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.147"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            56..271
                     /gene="bdm"
                     /locus_tag="ACHJ7J_004320"
     CDS             56..271
                     /gene="bdm"
                     /locus_tag="ACHJ7J_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO5002469.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            373..510
                     /gene="sra"
                     /locus_tag="ACHJ7J_004321"
     CDS             373..510
                     /gene="sra"
                     /locus_tag="ACHJ7J_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO5002470.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            667..2364
                     /gene="maeA"
                     /locus_tag="ACHJ7J_004322"
     CDS             667..2364
                     /gene="maeA"
                     /locus_tag="ACHJ7J_004322"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5002471.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLVG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2498..3508
                     /gene="adhP"
                     /locus_tag="ACHJ7J_004323"
     CDS             2498..3508
                     /gene="adhP"
                     /locus_tag="ACHJ7J_004323"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO5002472.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3655..3939
                     /gene="yddM"
                     /locus_tag="ACHJ7J_004324"
     CDS             3655..3939
                     /gene="yddM"
                     /locus_tag="ACHJ7J_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5002473.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4167..4820)
                     /gene="fdnI"
                     /locus_tag="ACHJ7J_004325"
     CDS             complement(4167..4820)
                     /gene="fdnI"
                     /locus_tag="ACHJ7J_004325"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO5002474.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4813..5697)
                     /gene="fdnH"
                     /locus_tag="ACHJ7J_004326"
     CDS             complement(4813..5697)
                     /gene="fdnH"
                     /locus_tag="ACHJ7J_004326"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO5002475.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5710..8757)
                     /gene="fdnG"
                     /locus_tag="ACHJ7J_004327"
     CDS             complement(5710..8757)
                     /gene="fdnG"
                     /locus_tag="ACHJ7J_004327"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8170..8172),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5002476.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGMLIAKKGQLLRSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8989..9870
                     /gene="yddG"
                     /locus_tag="ACHJ7J_004328"
     CDS             8989..9870
                     /gene="yddG"
                     /locus_tag="ACHJ7J_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO5002477.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2424 a         2626 c         2515 g         2403 t
ORIGIN      
        1 tataaatctg cgatccgtag cagacactat aaatacacag acacggagaa tcactatgtt
       61 tacttattat caggcagaaa attcaacagc agaacccgct ctggttaatg ccattgagca
      121 aggccttcgg gcagagcacg gtgttgtcac tgaagatgac attctcatgg agctgaccaa
      181 atgggtggaa gcttctgata acgacatcct cagtgatatc taccagcaaa cgatcaatta
      241 tgtggtcagt ggccagcacc ctacgcttta aggtgctatg cttgatcggc aacctaattt
      301 aggggtttag cacgtgtttc ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat
      361 tgaggagtta aaatgaaatc gaaccgtcag gcacgtcata ttcttggact ggaccataaa
      421 atttctaacc agcgcaaaat agttaccgaa ggtgacaaat ccagcgtggt aaataaccca
      481 acaggcagaa aacgccccgc tgaaaagtaa ttcataacca tcagtcctca atgacgatta
      541 aacaccattg cctgcgcaat ggtgtttttg tttttatctg ctttatactt ggggccgacg
      601 ccctggcggt aaagcaaaga cgataaaagc cccccaggga tggatattca aaaaagagtg
      661 agtgacatgg aaccaaaaac aaaaaaacag cgttcgcttt atatccctta cgctggccct
      721 gtactgctgg aatttccgtt gttgaataaa ggcagtgcct tcagcatgga agaacgccgt
      781 aacttcaacc tggtggggtt actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa
      841 cgagcatgga tccagtatca gggattcaaa accgaaatcg acaaacacat ctacctgcgt
      901 aacatccagg acactaacga aaccctcttc taccgtctgg taaacaatca tcttgatgag
      961 atgatgcctg ttatttatac cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc
     1021 taccgccgtt cacgcggcgt gtttatctct taccagaacc gccacaatat ggacgatatt
     1081 ctgcaaaacg taccaaacca taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt
     1141 ctggggcttg gtgaccaggg catcggcggg atgggcattc cgatcggtaa actgtcgctc
     1201 tataccgcct gtggcggcat cagcccggcg tatacccttc cggtggtgct ggatgtcggc
     1261 accaacaacc aacagctgct taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc
     1321 actgacgacg aatactatga attcgttgat gaatttatcc aggctgtgaa acaacgctgg
     1381 ccggacgtgc tgttgcagtt tgaagacttc gcacaaaaaa atgcgatgcc gttacttaac
     1441 cgctatcgca atgaaatttg ttcctttaac gatgacattc agggcactgc ggcggtaaca
     1501 gtcggcacac tgatcgcagc aagccgcgcg gcaggtggtc agttaagcga gaaaaaaatc
     1561 gtcttccttg gcgcaggttc agcgggatgc ggcattgccg aaatgatcat cgcccagacc
     1621 cagcgtgaag gattaagcga ggaagcagca cggcagaaag tctttatggt cgatcgcttt
     1681 ggcctgctga ccgacaagat gccgaacctg ctgcctttcc agaccaaact ggtgcagaag
     1741 cgcgaaaacc tcagtgactg ggataccgac agcgatgtgc tgtcactgct ggatgtggtg
     1801 cgcaatgtaa aaccagatat tctgattggc gtctcaggac agaccgggct gtttacggaa
     1861 gagattatcc gtgagatgca taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac
     1921 ccgacgtcac gcgtggaagc cacaccgcag gacattatcg cctggactga aggtaacgcg
     1981 ctggtcgcca ctggcagccc gtttaatcca gtggtatgga aagataaaat ctaccctatc
     2041 gcccagtgta acaacgcctt tattttcccg ggcatcggcc tgggggttat tgcttccggc
     2101 gcgtcacgta tcaccgatga gatgctgatg tcggcaagtg aaacgcttgc tcagtattcg
     2161 ccgctggtcc tgaacggcga aggtctggta ctaccggaac taaaagatat tcagaaagtc
     2221 tcccgcgcaa ttgcgtttgc ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa
     2281 acgtctgccg aagctttgca acaagccatt gacgataatt tctggcaagc cgaataccgc
     2341 gactaccgcc gtacctccat ctaagcctgc gcccggtagt gaaggctacc gggcaatttc
     2401 cctctccctt tttcagatct catccatact gggtagtggc gaataaatat catttgcctc
     2461 acctgctatg cagaacatca tccgaaaagg aggaactatg aaggctgcag ttgttacgaa
     2521 ggatcatcat gttgacgtta cggataaaac actgcgctca ctgaaacatg gcgaagccct
     2581 gctgaaaatg gagtgttgtg gtgtatgtca taccgatctt catgttaaga atggcgattt
     2641 tggtgacaaa accggcgtaa ttctgggcca tgaaggcatc ggtgtggtgg cagaagttgg
     2701 cccaggtgtc acctcattaa aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg
     2761 atgcggtcat tgcgaatact gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa
     2821 tgccggatac agcgttgatg gcgggatggc ggaagagtgc atcgtggtcg ccgattacgc
     2881 ggtaaaagtg ccagatggtc tggactcggc ggcggccagc agcattacct gtgcgggggt
     2941 caccacctac aaagccgtta agctgtcaaa aattcgtccc gggcagtgga ttgctatcta
     3001 cggtcttggc ggtctgggta acctcgccct gcaatacgcg aagaatgtct ttaacgccaa
     3061 agtgatcgcc attgatgtca atgatgagca gttaaaactg gcaaccgaaa tgggtgcaga
     3121 tttagcgatt aactcacgca ccgaagacgc cgccaaaatt gtgcaggaga aaaccggtgg
     3181 cgctcacgct gcggtggtaa cagcggtagc taaagctgcg tttaactcgg cagttgatgc
     3241 tgtccgtgca ggtgggcgtg ttgtggcagt cggtctgccg ccggagtcta tgagcctgga
     3301 tatcccacgc cttgtgctgg atggcattga agtggtcggt tcgctggtcg gcacgcgcca
     3361 ggatctaacc gaagccttcc agtttgccgc cgaaggcaaa gtggtgccga aagtcgccct
     3421 gcgtccgtta gcggacatca acaccatctt taccgagatg gaagaaggca aaatccgtgg
     3481 ccgaatggtg attgatttcc gccgctaaga ggcctttgct gcgactgcca gtcctggtgt
     3541 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaagaagg
     3601 aacaataatg atagtgttcc cagtgaagga acactgctga gagtaaagac actgatgaaa
     3661 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3721 agcctgcgcg agtttgccag agcaatggaa attgctccct caacggcaag ccgattgctg
     3781 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3841 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc tgaaaaaacg
     3901 gtggatgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3961 ccatttgaaa ctgatgacaa atgcaacatt gcctgatgcg ctacgcttat caggcctaca
     4021 cgttcactgc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     4081 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     4141 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     4201 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     4261 ttcccttcga tcatcccttt aatcgatcct ttcacccaga atgccatata catatggatc
     4321 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     4381 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     4441 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     4501 ccgacatccg ccactttatg ctcattgcct ttcaatactt cgacaatgtt taacagccac
     4561 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4621 gcgacgaaaa tcgcaatgcc gaagaatggg tgcaaaatgc gtcccatctg cggcgtaccg
     4681 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4741 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4801 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4861 aataccgatg taatggaaaa tcaaccctgc aaaagtggca ataaagccag ccgctgccag
     4921 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4981 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     5041 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     5101 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     5161 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     5221 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     5281 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     5341 aatgattgca ccagcagacg ggcacgcctt aaggcagccc ggatcttcac agtgcataca
     5401 gccgtcttta cggatcagcc actccagctt gccgttctgt tcggtttcgc taaagcgcat
     5461 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     5521 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacaaccgat
     5581 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5641 cggcgtgatg gagttagttg cggacctttt gataatgtcc tgcgtttcca tagccatctg
     5701 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5761 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5821 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5881 cggtttcaga cggcgcgtta ccacagccac cgcgcggata aagccacgct tgcttgagac
     5941 agtgacacga tcgccattat taatgccttt cgccgccgcc agcgtttcgc tgatttccac
     6001 aaactgttcc ggctgagcaa ttgcgttgag caatgcgtgc ttggtccagg tgtggaagtg
     6061 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     6121 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     6181 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     6241 gttgatggca aacaggcgtc ccatcccttc cggctgcatg ataaacggcc cggttggcgt
     6301 acccggtgcg gcattgccga agtcaggaat atcgttaccc gtccacttgc tgccgttcca
     6361 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     6421 gtacagcacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     6481 ggacgggtcg gagttatcgc ggttagccat ctggttgccc tgctctgtcc agctaccggt
     6541 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctacgcag
     6601 caactgacct ttcttcgcaa tcagcatgcc attagcgtca tagagatctt ccagcgcata
     6661 gccgttgttc tcttttgcca cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6721 gctcatcttc atcagcggtt ctacgccttt accaccttca gcctggtaca gctcgcgcag
     6781 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcatcctg
     6841 acctttccag tgccactgca gccagcgacc ggagttagca atagaaccat cttcttcagc
     6901 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6961 cgactcgccg tggttctgcc agaaggttga ggtttcagtc accagcggat cgataaccac
     7021 catgtacttc agcttgctca ggcagctcac cactttgttt ttgtccggga aggacgcaac
     7081 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     7141 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     7201 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     7261 gctccagtag ttcacctgac cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     7321 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     7381 cagacctaag tcagtcagac cctgaatgtt ggagtgacca cgcaatgcat tcacgccgcc
     7441 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     7501 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7561 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7621 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7681 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7741 gttccaggac gatttatcgt attgccgttt ttcagcgtcg tagccgctga acagaccgtc
     7801 ttcgaaagca aaatcatcac gcaccagcag gctggcgttg gtgtaatgct taacgtattc
     7861 ggcgttgatt ttgtcgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7921 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7981 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     8041 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     8101 atccacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     8161 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     8221 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     8281 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     8341 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     8401 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcgctgt tgacgtaatc
     8461 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     8521 ttcaatgtga taaatcgctt ctctggcgtt tttcgcgcca tcacccaggc tatacatcaa
     8581 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8641 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctactgt
     8701 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8761 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8821 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8881 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8941 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     9001 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     9061 cgcggtgtca gcgaggggct aggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     9121 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcaaatccc gaaaggctat
     9181 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     9241 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     9301 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggtt gattgtacct
     9361 ggattattat tagccctcgt cggcgtctgc tgggtgttag gcggtgacaa tgggttacat
     9421 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgttcatt
     9481 ggtgcgttta tctgggcagc ctactgcaca gtaacgaata aatacgcccg cggatttaat
     9541 ggaattaccg tttttgtcct gctaacggga gcaagtctgt gggtttacta ttttctcacg
     9601 ccacaaccag aaatggtatt tagcacaccc gtcatgatta aactcatctc tgcggcattt
     9661 accttaggat ttgcttatgc tgcatggaat gtcggtatac tgcatggcaa cgtcaccata
     9721 atggcggtag gttcgtattt tacgcccgta ctttcctcag cgctagcagc cgtgctgctc
     9781 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctga tggtctgcgg cggttccctg
     9841 ctctgctggc tggcgacacg tcgtggctaa aataattgcc gggtctacgg cccggcaatt
     9901 aatcagataa accccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9961 atctcatt
//
LOCUS       DAZFFG010000148         9915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFFG010000148 DAZFFG010000000
VERSION     DAZFFG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.148"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..135)
                     /locus_tag="ACHJ7J_004329"
     CDS             complement(<1..135)
                     /locus_tag="ACHJ7J_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002478.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPYG
                     E"
     gene            213..1307
                     /gene="ddlA"
                     /locus_tag="ACHJ7J_004330"
     CDS             213..1307
                     /gene="ddlA"
                     /locus_tag="ACHJ7J_004330"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5002479.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFNVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFTEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1331..1543)
                     /gene="yaiZ"
                     /locus_tag="ACHJ7J_004331"
     CDS             complement(1331..1543)
                     /gene="yaiZ"
                     /locus_tag="ACHJ7J_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO5002480.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1803..2111
                     /gene="yaiY"
                     /locus_tag="ACHJ7J_004332"
     CDS             1803..2111
                     /gene="yaiY"
                     /locus_tag="ACHJ7J_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO5002481.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2170..3264)
                     /gene="yaiW"
                     /locus_tag="ACHJ7J_004333"
     CDS             complement(2170..3264)
                     /gene="yaiW"
                     /locus_tag="ACHJ7J_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO5002482.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPANVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3277..4497)
                     /gene="sbmA"
                     /locus_tag="ACHJ7J_004334"
     CDS             complement(3277..4497)
                     /gene="sbmA"
                     /locus_tag="ACHJ7J_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO5002483.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4849..6006
                     /gene="ampH"
                     /locus_tag="ACHJ7J_004335"
     CDS             4849..6006
                     /gene="ampH"
                     /locus_tag="ACHJ7J_004335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO5002484.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6007..6630)
                     /gene="iprA"
                     /locus_tag="ACHJ7J_004336"
     CDS             complement(6007..6630)
                     /gene="iprA"
                     /locus_tag="ACHJ7J_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO5002485.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6718..9735)
                     /gene="ehaB"
                     /locus_tag="ACHJ7J_004337"
     CDS             complement(6718..9735)
                     /gene="ehaB"
                     /locus_tag="ACHJ7J_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO5002486.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGADSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMEDRGTDANNDGVFEYTVDLDGDGILDHTTPDGVYDSYGVGTEA
                     TATLLDIAANSLWPSSTVGVDNINTQYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTK
                     SSLIDTLSINSGSQVNVADSRLISDTISLTGGSNLNIGEDGHVATNTLTIDNSTVKMS
                     DDVSAGWGLEDAALYANTITVTNDGLLDVNVDQFDANPFQADTLNLTSTTDTNGNIHA
                     GVFDIHSSDYVMDTDLVNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEA
                     GQNEVDNNGDHVAAATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKA
                     DLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHG
                     LADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKG
                     DMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTN
                     SDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYEL
                     GVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFS
                     FTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2549 a         2461 c         2359 g         2546 t
ORIGIN      
        1 ctcaccgtat ggccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat
       61 cttgcgcaca tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc
      121 agaagattgc aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat
      181 gcactttgtg ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt
      241 ttggtggtaa atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg
      301 ccattgataa aagtcgcttc aacgttgtgc tgctgggcat tgataaacaa gggcaatggc
      361 acgtcagcga tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc
      421 gcccttcggc gaccagcctc gcccaggtgc caggtaaaca tgagcatcag cttatcgacg
      481 cgcaaaacgg tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc
      541 tgggcgaaga tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt
      601 ctgatgttct ggcttcagca gcctgtatgg ataaagatgt aaccaaacgt ctgctacgcg
      661 atgccgggct gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca
      721 gttttaccga agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg
      781 gctcttctgt tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc
      841 tggcgttcga gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg
      901 aatgcgcagt tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca
      961 ccagcgattt ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag
     1021 ttccggcagc cattgcgcca gaaatcaacg ataagatccg ggcgattgcc gttcaggctt
     1081 atcagacgtt gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg
     1141 aagtggtgat caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga
     1201 agctgtggca agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg
     1261 cgctggagcg tcacgctgcg gataacgcat tgaaaaccac aatgtaatat tttaacgccc
     1321 gataatattc ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg
     1381 ccagccagaa actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg
     1441 cttcaaatcc cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg
     1501 catagtagtg ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga
     1561 aaaacaacat ccttgcatat tctgcaacaa ccgtctgtgg tgtagacaac attttcgcaa
     1621 ttgttaataa atttaactta aagttgctat tttgtgactc gcagcgaaga ttcacgcttc
     1681 gggaatattc ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc
     1741 agacttgcca taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt
     1801 ttatggctga tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt
     1861 caacgcctgg caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc
     1921 aattgctgaa cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg
     1981 aaacaggtcg cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc
     2041 cgtttttagt aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc
     2101 gccgccagta agatttacgt tgccagatac ggcctaaaac gccttatctg gcatgacatt
     2161 cctgtttgat cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca
     2221 ggccgtagtc aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc
     2281 gcgggggagt gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc
     2341 cgtctcctca aaagagaagc tgtcaccttt ctctagctga cgacgaattt cgctgtcgct
     2401 catccccagt tttgccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact
     2461 gtcgtaacga attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgaccgc
     2521 gttctgaaac gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaaacggta
     2581 tatcggtgcg ctataactgg cgggataatt cagtaaatgg taagtaccaa accacaaccc
     2641 gccgcggcga ctaaagactt cctgacggac tgtaccgtcc attttccacg gatacccttt
     2701 ggtatgctgt tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt
     2761 aagcgaacca aacagcgtct gccccattgg caccatgctg atcagatcgt caaaaatcgc
     2821 gctcaattgc ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg
     2881 cgatttgatt tttagcgcgg tatgaaccag aaaaggagga atgtgcatcc gttcagcacg
     2941 acggtcaatc tcttgccagg cgattttgct taagcccgga acggccggat ctgcctgata
     3001 atttgactcc tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag
     3061 cccctgactt tcaaaggtgg tggcgagatc ttttgcccag gcatcacggt ctttcacgtt
     3121 cgcaggcatc ttctgccgca cgacgctcgc cacatctatc gctttttcgc cctttttcaa
     3181 cggttgcgga gcctgactac tgcaccccgc taaaaccaga accgccaaca atgacagcga
     3241 agatagagga ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc
     3301 ctgaattttg tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat
     3361 caactcaacc agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc
     3421 aaaaacgttg gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg
     3481 aaacagcaag aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gagcgatgtt
     3541 gaaatacata tagtgaaaat agaggcggaa atagtttttc cgtacggcgc taaacagctc
     3601 gcgtaccgtt ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg
     3661 gtaggcagcc tctacacgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac
     3721 tgccagcaat ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg
     3781 aatgtgcccg ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag
     3841 gaaggcgatc aacgtcatga tggcattgat aaaactgaca cccatattct ccagcgttga
     3901 agcaaaacgc atggtgtctt cctgcacacg ctgtgcggcg ccttcgatat gacgcagttg
     3961 ttgccagttc gccatgtaat attcgttcat cgccgtacgc cagcggaaca cgtagtgact
     4021 gacaaagaag ttattcagca cactgatcac taccgcgatc agcgcaatcc ccagaaagac
     4081 gcccacttcg cggtaaaatt gttcgatggt gactttatgc ggcgaactta gcgcggtttg
     4141 aatcagatca tagaacggtg cataccaggc gttgacggca accccgactt ccaccaaaaa
     4201 ccaggtgaca aagataatca gcgcagtacc gagtattgac cagtattgcc aacgatgcgg
     4261 gctgtagata aaccagaaca atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa
     4321 aatcaggaaa tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagctcc
     4381 ggtgatacgc gccacccagt cacccccacc ggcttgccag aagataacgg caatcaatgc
     4441 ccaaacaaag gccgagagaa aaaacgttcc cggctttggg aaaaaagact taaacatatg
     4501 cactcctgct aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccgctac
     4561 aaagcgtcag ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag
     4621 cctggccata agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga
     4681 ttaacacgcg caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg
     4741 cgccgttacg tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag
     4801 tagtataaat acgctcagtt accttcattc aatttatgga caccaccgtt gaaacgtagt
     4861 ctgctttttt ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg
     4921 atcactgaac cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac
     4981 ggcagcggtg ccacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgc
     5041 agttatggcg aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt
     5101 attgcttccc tcaccaagct aatgaccagt gagatgctgg tgaaattgct cgaccagggg
     5161 accgtgaagt taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccaacc
     5221 tacaatggga cgcccattac gctggtcaat ctggcaaccc ataccagcgc cctgccccga
     5281 gaacagcccg gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc
     5341 tggaaatacc tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct
     5401 aaccttgcgt tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc
     5461 cagttgtttg aagagcagat tacccgtcca ttggggatga aagacaccac ctacacccca
     5521 tcgccagacc agtgccgccg tttaatggtt gcagagcgcg gtgccagtcc atgcaataac
     5581 acgctggcgg caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg
     5641 atgcagcagt atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag
     5701 acgttgattt atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa
     5761 gcggatgcgc ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt
     5821 attcagaaga caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag
     5881 aatattggcg cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc
     5941 gatggcatca atgacctggt gaccgagcta agcgggaata aaccgctggt tatccccgca
     6001 tcctgattaa tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc
     6061 gatatagccg cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg
     6121 tgtacgttga tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc
     6181 aatcattgat aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgcac
     6241 gtcgcgtaat tccagaattc tattttgcca ggctaaccag taaaaagcgt ctcgccaaag
     6301 ttgattttgt tcaataagcg taatggtttg tttggctggt agatgatatc ccgtacaatt
     6361 tccttctgat attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat
     6421 aatataagga gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac
     6481 tccctctaaa ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt
     6541 ttcattatta aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa
     6601 ttcctgaaga ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa
     6661 atccttgacg ggcattattt agattaaatt accagtattt cttcggactg aagaatatta
     6721 ccaggtatat ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag
     6781 gtagttagca tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc
     6841 aacacgtacc gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc
     6901 gttgttagag tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc
     6961 gctgtaggtg aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga
     7021 ctgaccgtca actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc
     7081 cgaaatgctg ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggc
     7141 tttcaaaccg aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct
     7201 catggttgca gacaggtcgt tgttgaagtg agagtagctc aagctaccat caacaaagac
     7261 gttgttcgca aagtgagcag aagagtagat gtaggcagtc tggctgtctt gatccacctg
     7321 accagaacgg tcattcatgt cacctttagc gaagcctgca gccgcaccga cgatccactt
     7381 agcgttgtta ccgtcaattt tggtatcaac accgaccatg atgccgttaa catcctgatc
     7441 atagttgatg gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc
     7501 gccgttatca gccaggccat gacgagagtt ggtcaaacga gtaccaacgg tgtcttgttc
     7561 caggttccag atattggtgt tcgcagatgg gatgctcagc gccatgttag cgtagtcggt
     7621 caactccatc tgttgcagaa caacggtgtt accgcgctgt tcagcctgat aggtgtatgc
     7681 acccaggtca gctttgttag cagcagagaa ggtcgcgttg ctgtttttgt cgttgacgta
     7741 gatcagctcg ttgccgttgt agtcagcgat agaaccagca ccagtagcgt tgtcgatacg
     7801 aactttgtag ttaccggttg cggctgcaac atggtcaccg ttgttatcaa cttcgttctg
     7861 accagcttcg atagaagcag tgttgtcgtt atcgccgtta ccgttaatgg tcaggtgacc
     7921 atcagagttc attgcgatta agccataacc gtagtttgac ttggtagtat cgttggtacg
     7981 gtcgttgacc agatcggtat ccattacgta atcactgcta tggatatcga atacaccagc
     8041 gtgaatgttg ccgttagtat cagtggtact ggtcagattc agggtatcgg cctggaacgg
     8101 gttagcatcg aactgatcaa cgttaacatc caacagaccg tcgttagtta cggtgatggt
     8161 atttgcgtac agtgcagcat cttctaaacc ccagcccgca gaaacatcat cagacatttt
     8221 aacggtacta ttgtcgatgg tcagggtatt agtcgctaca tgaccgtctt caccgatgtt
     8281 caggttagaa ccgccagtca gagagatagt gtcagagatc agacgagagt ctgcaacatt
     8341 aacctgggaa ccactgttaa tacttaaagt atcaatcaga gaagattttg ttgtatccca
     8401 ctctgaacca ccgttcaaag tcacattaaa caaaccgctc tggtaaactt cgtttcctac
     8461 gatatggcca ttttcgtcat attgagtatt gatgttatca acaccgacag ttgatgaagg
     8521 ccacaggctg ttagctgcaa tatcgagcag agttgcagtt gcttcagtac caacaccgta
     8581 gctgtcgtaa acgccatctg gtgtagtgtg atcaaggata ccgtcaccgt ctaagtcgac
     8641 tgtatactca aatacgccgt cgttgtttgc gtcagtacca cggtcttcca tatgaacaga
     8701 cattgcagca ccaacccact tgctgccgtt gttcagagtc acatccatac ggtctgtgcc
     8761 atcccaaccg ttggtatcta catcaccatc agcatcgcga tagctgtcag caccttgcgg
     8821 gaagaagttt tcatcgaaat tactggagaa aacaacatca cccatcagtg ttgagttgtc
     8881 taaagttact gtagtctgca tcgcattatc agcatacgga tttgcgattg cggcaattgc
     8941 aacatcgtct gcagtcagcg tgttgctata gttgctggca ttaccagtat ggccaaacca
     9001 accagtagta ccttcatcag tccatgaacc agaggtcaca gtagaatctt taacagtaat
     9061 agtgttatta aacacttcgc tgctgtttac gccagtgctg actgtgttgt cgtcaatatc
     9121 ttcccactca taaccctgag tcagcgtaat acctgctaca tgagagttat tagtgatgga
     9181 cagatcaact tcctgatcca gtgtaatagc agtacccata tcgtaaacat ctacaacatg
     9241 agtgtcagca gcattattat aggtaccgtt gtaagtataa tgctcgtagt tgtcatcgat
     9301 cgttgaatta tcaacgctca gtgtcagacg atcataaaca taaccagtag cacggtcatc
     9361 agcacaatca gtagtcatgc actcagaggt aatcataccg tgaatagtgc tgtttttgat
     9421 tgtcagacta ttggcgttgg tattggtaga aataccgtcg tccagatagt aggtagaaat
     9481 cacgccgtta acggtagcgt tattaatgac cgggtagata tcgccgttgt aatagctatc
     9541 agcggcatag ttgttccagc ctacataacc atcatagtaa acgtcatcag cataggtggc
     9601 gtcgttgtag tgatggaaag tattgtaagt ttgaccagaa atatcgttgg tcgctgcatt
     9661 agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta caagtttctt
     9721 tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc catcaattat
     9781 caatgcatag aaaacatctt atgtttcgcg ttgacgatta tgcggcgtta tagaggaatg
     9841 gttcaattat tctttcccct aagtccgatt gcggacatat ataaaaatat ctatacgcac
     9901 aaagaataag ttgac
//
LOCUS       DAZFFG010000149         9865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFFG010000149 DAZFFG010000000
VERSION     DAZFFG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.149"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            6..81
                     /locus_tag="ACHJ7J_004338"
     tRNA            6..81
                     /locus_tag="ACHJ7J_004338"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHJ7J_004339"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHJ7J_004339"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /gene="yhdZ"
                     /locus_tag="ACHJ7J_004340"
     CDS             complement(299..1057)
                     /gene="yhdZ"
                     /locus_tag="ACHJ7J_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO5002487.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /gene="yhdY"
                     /locus_tag="ACHJ7J_004341"
     CDS             complement(1065..2168)
                     /gene="yhdY"
                     /locus_tag="ACHJ7J_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5002488.1"
                     /translation="MTKVLLSHPPRPASHNSNRAMVWVRKNLFSNWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /gene="yhdX"
                     /locus_tag="ACHJ7J_004342"
     CDS             complement(2178..3359)
                     /gene="yhdX"
                     /locus_tag="ACHJ7J_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5002489.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRIFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3427..4452)
                     /locus_tag="ACHJ7J_004343"
     CDS             complement(3427..4452)
                     /locus_tag="ACHJ7J_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5002490.1"
                     /translation="MKKMMIDTLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4883..5104)
                     /locus_tag="ACHJ7J_004344"
     CDS             complement(4883..5104)
                     /locus_tag="ACHJ7J_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002491.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5357..8461)
                     /gene="acrF"
                     /locus_tag="ACHJ7J_004345"
     CDS             complement(5357..8461)
                     /gene="acrF"
                     /locus_tag="ACHJ7J_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO5002492.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8473..9630)
                     /gene="acrE"
                     /locus_tag="ACHJ7J_004346"
     CDS             complement(8473..9630)
                     /gene="acrE"
                     /locus_tag="ACHJ7J_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO5002493.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2624 a         2545 c         2367 g         2329 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttatgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga cttaggatgc gcaaaaaact
      361 catccggtgc agcttgctcc actatttcgc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgggtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gatgtgggct
      541 catcaaacaa cataattttc ggcttcatac acagcgagcg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctaccac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaacctgaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacagaa cggtaatcag cggcacgccg
     1561 cgccagaatt cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaactga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagagc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg acacaaggcc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagcc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccagttag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagttgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggtttga gttatggctc gccgggcgcg ggggatgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctgatt cagcacggtg ccggcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggt
     2401 ggaataatca cccgcaatgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat ccccgcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aatgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggcg
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gtgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcaaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccaacttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca tttagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagccggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggca acgttgagtt cggtatcagt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 ataagtgacg ccagtaaatg ccattcccat cccggcgtcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cttcccctga ctgtaaagcc gtgaagcgtt cttttgctgt
     4201 gagtggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aatatcaaca tcaatacctg aaaatttacc gtcagcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctga acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tgtctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatg ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aagtgcgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaatcag cagagaatta
     4801 gccagcagaa tatagcaata ttttatctta tgtaaagata ctgtatcgtt aaacagagcg
     4861 cgaaacttcg cgccctgttc agttatttcg acagagcgat gatccccatc gtcaggccag
     4921 caacagctgc tgctccgcca gcaatagccc cagccgtttc ccagttatcc tgagttgtgc
     4981 ctttcataca ggtattggtg tgaaccagac gcccctgatc gtcataaacc ggaacgcagg
     5041 gcgaatcgtg agcacagccc gccagcaatg cggtgagcaa tgccacagga attaatcttt
     5101 tcatggtgtt acctcagtaa taaacgctaa cgtttaataa gttaactctc ttattaacga
     5161 gctgaatgcc attttatcac cggaggttat aaatcttgcg gaatgaataa tgacaaatta
     5221 tgtgaattgt aaaaatagtg aagggagcgg gaactataga aagcttttaa agagtgagca
     5281 aaagaatatc ttattaattt ttatgggttt cactgaaaat aataaaggca cccgaaagcg
     5341 cctttatgtt tcagatttat cctttaaagc aacggcggat caccacaaag aacaccggta
     5401 cgaagaagat tgccagcaac gttgcagaga ccattcctcc cattaccccg atacccactg
     5461 cgttctgcgc gccactgccg gcaccgttac tgatagccag cggtaatacg ccgagaataa
     5521 aggcgagaga ggtcatcagg ataggacgca gacgcatacg tactgccatt agtgtcgctt
     5581 caacaacacc tttaccctct ttctccatga gatctttagc gaactcaaca atcaaaatag
     5641 cgtttttggc cgacaagcca attgtcgtta gcaagcccac cataaagtag acgtcatttt
     5701 tttgattaaa gagtgtcgcc gccagcagca cgccgacaat ccctaacggc acgaccaaca
     5761 taaccgagac aggaattgac cagctttcat agagtgcagc aaggcacaag aaaacaacca
     5821 caaaggaaat tgctaccaga gcgggagcct ggtttcccga taagcgttcc tgatacgaca
     5881 tacccgtcca gtcataacca atgcccgcag gtaattttga cgctaggttt tccatcaacg
     5941 ccatggcatc gccggaactg gttcctggcg cggcttcccc ctgaatctcc attgacggca
     6001 gaccgttgta gcgttccagt cgcggagagc catacaccca atgtgaagtg gtaaaggcag
     6061 agaatggcac catttcgccg ttggcgctgc ggacataaag tttatcgaca tcttctggca
     6121 gcatacggaa tttggcatcc gcctgaacat acaccttttt cacgcgacca cggtcgatga
     6181 agtcgttaac gtaagtccca cccagcgccg ttgaaatggt ctgattgatg tcagaaagtg
     6241 aaacacctaa tgcctgcgcc ttttcctggt caacttccag tttaaactgt gcggtgtctt
     6301 ccaggccatt aggacgcacg ctgactaagc tggcaggatg ttgcgccgcc ataccaagca
     6361 actggttacg ggcctgggtt agggcatcgt gacccagccc agcctgatca attaactcaa
     6421 agtcgaaacc cgttgccgtg cccagttcaa caatggctgg catattgaat ggaatgacaa
     6481 aaccgtcgcg gatcttgccc aactccattt tggcacgatg gattaccgct tccgcactgt
     6541 tttcgtcacc actacgctct tcccacggtt tcagactgac gaaggccata ccggcgtttt
     6601 gtgcctggcc gctgaagcta aagccgttaa ccgtaaagac actttcaacg tttgctttct
     6661 cgttcttcag atagtaatcc gtcacttgat ccaacacttt ttgcgtccgc tcttgcgtcg
     6721 cgccagcggg taactgaatc atggtcagaa agacaccctg atcctcttca ggtaagaagg
     6781 aagacggaag acgtaaaaac aacaccacca ttcctgcaac aatcagcgca tagatcagta
     6841 aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt ggtgtagtgg ttaacgctat
     6901 gatcgaaggt ggtattaaac cagccgaaga aaccaccctt attttcgtga tgctcagctg
     6961 aggtgggttt gagcagtgtt gcacataacg caggggtaag aatcaatgcc accagaacag
     7021 aaagcgccat tgccgaaacg atggtgatag agaactggcg ataaattgcc ccagtagaac
     7081 cgccgaagaa tgccatcgga ataaataccg ctgacagcac catcgcgata cccaccagtg
     7141 cgccctggat ttgcgacatt gatttttccg tcgcttcttt tggcgggagc ttatcctcca
     7201 tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc atcgacgagc agcccgatgg
     7261 caagcaccat cccgaacatc gttagtgtgt tgatggagta accaaaagcg gcgaggatgg
     7321 caaacgtccc taacaacacc acgggtaccg caatggtggg gatcagcgtt gctcgcatat
     7381 tctgcaagaa cagatacatc accaggaaca ccagcataat agcttcgaac agtgttttta
     7441 ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc ataagggtag agaaccttca
     7501 ttccctgcgg gaagaatggc tgtaattccg ccagttttgc cttaatggct ttcgcggtat
     7561 cgagagcgtt cgcaccggtt gccagcttaa tacccaggcc cgccgccggt tttccgttga
     7621 tgcgagcgat aacgttatag ttttcaccgc caagttcaac ccgtgcgaca tctttcagac
     7681 gcaccaccga gccgtcactg tttacgcgca gggtcacttt gccgaattct tccggatttt
     7741 ttaaccgcgt ctgagcaata atcgaggcat tcaactgttg ccctggtaac gctggcgttc
     7801 cgcccaactg tccggcagcg atctgatcgt tctgtacctt caactggtta atcacatcaa
     7861 ccggtgtcag tttatatttg tttagcagat cggcgtccag ccagatacgc atcgcatact
     7921 gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg gctaagcgta tctttaacgt
     7981 tagaggccac atagtccgag atatcgtcct gtgtggtgtc tgggttatca gagacaaagc
     8041 ccgccaccat caaatagctg ctactggact tttcaacact gatcccctgc tgctgaacct
     8101 cctgcggcag caacggcgtg gcgagctgga gtttgttctg cacctgcact tgcgcgatat
     8161 caggatcggt cccggactgg aaggtaaggg taattgtcac gctaccggcg gaatcgctgg
     8221 tggaggacat atacatcagg ttatcgatac cgttcatatt ctgttcgata acctgcgtca
     8281 ccgtatcctg cacggtctgc gcatcagcgc ccggatagtt tgctgaaaca gaaaccgcag
     8341 gcggtgcaat ggttggatac tgagcgacgg gcaattgtag gatcgccagt gcgcccgcca
     8401 tcatcagaat aatggccagc acccatgcaa atatcggtcg tcgaataaaa aagtttgcca
     8461 tgtcagatta ccttacttcg atgcagtatc tgcgggggta tcggtagtgg ctttaacctg
     8521 ctctcccgga cgcgcttttt gcaggccgct gacaatgact tgatcgccag atttcagacc
     8581 ttcgctaatc aaccatttat cgccaatcgc ctgactggca acgacagggc gcacttccac
     8641 ctgactttta tcgttaacaa tcagcacggt tgcatcacca cgcggtgtgc ggctaacgcc
     8701 ttgttgcggg atgagaatgg cgtcaggttg gacgccttca tcaatccgtg cacgcacaaa
     8761 catacccggc aaaagcgtat gttgcgggtt agggaagaca gcacgtaggg ttatggagcc
     8821 ggtgctttca tcaacggtca catcggagaa ttgcagcgta cctttcaggg gataggtttg
     8881 accgttttcc atgaccaact ctacgttgct ggtggcgttt tccttatgca aatttccttg
     8941 ctctacggac tgcttcagcc tcataaaatc gttgctggat tgggtcacat caacatagat
     9001 aggatcgagc tgctggacag ttgccagttc agtcgtttgc ccattagtga caagagcgcc
     9061 ttcggtcaca gtcgatttgc cgatacgtcc gctaattggc gcagtcactt tggtataagc
     9121 aagattgatg cgagcgcttt cgactgtggc ttttgcggca atcacggcgg catcggcctg
     9181 gcgagcatca gcaatggcct ggtcgtactc ctgctggctg atgtatttcg tacccacgag
     9241 cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg gcggcttcac ttttcgccag
     9301 ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg ggatcgatct ggtacaggga
     9361 ctggcctgct tgcacatcgc tgccttcagt gaaattgcga ttcagtacga tcccgctaac
     9421 ctgtgggcga acttcggcta tacgataagc attggtgcgg cctggtaatt cagtcttaac
     9481 ttctaacggg gccgttttta caatatgaac ggtaacctgc ggttcaccga cgtgagcttt
     9541 ctcttctccc ttatcgttac aaccggcgat taaagccgcg gagatcagaa taaaggaggg
     9601 caggaggaaa aacctggcat gtttcgtcat tactattcct caaaaaacca aaagcgcgtt
     9661 atttacccaa aaaggcaacg cgttaactcg cagaaagaaa aatacagttc gctatcctac
     9721 aaattatcat tcgtcgatgt aaggaatagt tatgaatact ggcgtctcaa ggcacataaa
     9781 cacaaaaaaa gattaatatt ctactgtttt attttgacgc gggttgaaag aagcagaatt
     9841 aaaacctcgt aaattgaaat atata
//
LOCUS       DAZFFG010000150         9863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFFG010000150 DAZFFG010000000
VERSION     DAZFFG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.150"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..711
                     /gene="espY4"
                     /locus_tag="ACHJ7J_004347"
     CDS             <1..711
                     /gene="espY4"
                     /locus_tag="ACHJ7J_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY4"
                     /protein_id="HFO5002494.1"
                     /translation="MQENAQNIINNMIESDRIKFVESIINKMIPPPDGTMLTENPKEY
                     VQKQIKESHKESATNVTFDYKELASIFEGVEEKQIKALSNQLEHIKSFKTDYDSRLNK
                     YARDFHYFSSSFAINREDLLKNYQEIRASIKDYDDLLREIKELTIKRDKSVDDKRTLF
                     DNKRDKWDSQEVQDEVAALNQKIADDDNKIRSNLNIVQNFRRENQYKANKKISDAMRN
                     ILDWFERYPDIVKNITQA"
     gene            complement(848..1513)
                     /gene="yieH"
                     /locus_tag="ACHJ7J_004348"
     CDS             complement(848..1513)
                     /gene="yieH"
                     /locus_tag="ACHJ7J_004348"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO5002495.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMIQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELAQLPALWKARGWN
                     ITR"
     gene            1680..3017
                     /gene="adeP"
                     /locus_tag="ACHJ7J_004349"
     CDS             1680..3017
                     /gene="adeP"
                     /locus_tag="ACHJ7J_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO5002496.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(3071..3637)
                     /gene="yieF"
                     /locus_tag="ACHJ7J_004350"
                     /gene_synonym="chrR"
     CDS             complement(3071..3637)
                     /gene="yieF"
                     /locus_tag="ACHJ7J_004350"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO5002497.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDQQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKM"
     gene            complement(3659..4408)
                     /gene="yieE"
                     /locus_tag="ACHJ7J_004351"
     CDS             complement(3659..4408)
                     /gene="yieE"
                     /locus_tag="ACHJ7J_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO5002498.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKSFSPN"
     gene            complement(4565..5524)
                     /gene="yidZ"
                     /locus_tag="ACHJ7J_004352"
     CDS             complement(4565..5524)
                     /gene="yidZ"
                     /locus_tag="ACHJ7J_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO5002499.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            complement(5499..6674)
                     /gene="mdtL"
                     /locus_tag="ACHJ7J_004353"
     CDS             complement(5499..6674)
                     /gene="mdtL"
                     /locus_tag="ACHJ7J_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO5002500.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(6806..8053)
                     /gene="tnaB"
                     /locus_tag="ACHJ7J_004354"
     CDS             complement(6806..8053)
                     /gene="tnaB"
                     /locus_tag="ACHJ7J_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO5002501.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(8144..9559)
                     /gene="tnaA"
                     /locus_tag="ACHJ7J_004355"
     CDS             complement(8144..9559)
                     /gene="tnaA"
                     /locus_tag="ACHJ7J_004355"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO5002502.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(9780..9854)
                     /gene="tnaC"
                     /locus_tag="ACHJ7J_004356"
     CDS             complement(9780..9854)
                     /gene="tnaC"
                     /locus_tag="ACHJ7J_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO5002503.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
BASE COUNT         2608 a         2393 c         2416 g         2446 t
ORIGIN      
        1 atgcaggaaa atgcacaaaa tatcattaac aatatgatag aaagtgaccg gattaagttt
       61 gttgaatcta taataaataa aatgattccg ccgccagatg gaacaatgct tactgaaaat
      121 ccaaaagaat atgtccaaaa gcaaataaag gaatctcata aagaatctgc tacaaatgta
      181 acatttgatt ataaggaact tgcatccatt tttgaaggtg ttgaagaaaa acaaattaaa
      241 gcactatcaa accaactgga acatataaaa tcttttaaga cagattacga ctcaaggctc
      301 aataagtatg ctcgcgattt tcactatttt tccagttcat tcgcaattaa cagggaggat
      361 ttactaaaaa actatcagga aataagagct tccattaaag attacgatga cttattgcga
      421 gaaattaaag agttaaccat aaagcgtgat aaatcagtag atgataaaag gactttattt
      481 gataataaaa gggacaagtg ggatagtcaa gaagtacagg atgaagtagc ggcattaaat
      541 cagaagattg ctgacgatga caataagata cgttctaacc ttaatattgt tcaaaatttt
      601 agacgcgaga atcaatataa agctaataaa aaaatcagtg acgcgatgcg taatattctg
      661 gattggttcg aacggtatcc cgatattgtg aaaaatataa cccaggctta accatacaga
      721 gggtgtgcga ctggacgatt aacgacataa attatttata gggaatagac gcttactcat
      781 cagacacctg gtatttttta tctcaaacca gcgtgactga tgtgtaagcg ttgaagtaag
      841 gaggcgatta tcgcgttata ttccagcccc gtgccttcca gagtgcaggc agctgcgcca
      901 gctcggtaaa ggtggttact ttcgggtgaa cgatcggttt attgtgcggg tcagcgcaga
      961 agtaaaacac ttccattccg gcatctatcc ctgattgcgc accggcgctg gagtcatcga
     1021 ccagaatgca gttttcaacg ttcacattca tcgcttttgc cgcatggaac attaacgccg
     1081 gatcgggctt ccaacgctgg atatcgtagc cgctgaacag tttttccggg aagtagtgca
     1141 gcatgttgag cttacccagc gaatgctgca ttttgctgac cggaccgtta gagacgacgc
     1201 acatcggcac gttaatgctc gccagcaatt cgttcgctcc tgcgatgtcc tcaagttcgg
     1261 tatcaaacag acgcgccact tctgcgcggt aaacgtgctc ggcatcggcc tggatcatcg
     1321 tcacgccctg ttctttactg atgatgtcga tgatttcgta gagttttacg cctttaaaac
     1381 gggtaaaaat ctctttcagg tcaacgtgaa tgccaaattc cctgaacatc gtgacatagg
     1441 cgcgagagca aatgacctcg ctatcgacca gcgtaccgtc gcagtcgaaa aataccgctt
     1501 ctatctggga catgtctttc ctttatcgtt agtttaacgt tttcgtcatg ccaattcgca
     1561 tgacgcaatc gttgccgtat aagcaaatac aatgaaaaaa agttatcttc agccgtccat
     1621 attgtctcat tttggtatac gatagcgacg gattttccct ccttgttcgg aaatagataa
     1681 tgagtcaaca acacacaacc caggcttctg gccaggggat gctggaacgc gtgtttaaac
     1741 tgcgcgaaca tggcacgacg gcacggaccg aagtgatcgc cggttttacc accttcctga
     1801 cgatggttta catcgttttt gttaacccgc aaattcttgg cgttgctggc atggatacca
     1861 gcgccgtctt tgtcactacc tgtctgattg ctgcattcgg cagtattatg atggggctgt
     1921 ttgctaacct gccagttgca ctggcacccg ctatgggcct gaatgcgttc ttcgcttttg
     1981 tcgttgtgca ggcgatgggc ttgccgtggc aagtcgggat gggcgcaatc ttctggggcg
     2041 cgattggtct gctattactg acgattttcc gcgttcgcta ctggatgatt gccaacattc
     2101 cggtgagtct gcgtgtgggc attaccagcg gtatcggtct gttcattggc atgatggggc
     2161 tgaaaaacgc aggtgtgatt gtcgctaacc cggaaacgct ggtgagcatc ggtaatctga
     2221 cttctcacag cgtgctgctg gggatcctcg gcttcttcat cattgctatt ctggcctcac
     2281 gcaacattca cgcagcggtg ctggtttcta tcgtggtgac aacgctgctg ggctggatgc
     2341 tgggtgatgt ccactacaat ggcatcgttt ctgcgccgcc gagcgtaatg actgtcgtgg
     2401 gtcatgtaga tttagccggg tcgtttaacc tcgggctggc aggggtgatt ttctctttca
     2461 tgctggtcaa cctgtttgac tcctccggta cgttgattgg cgtgaccgat aaagcaggtc
     2521 tggcagatga gaaggggaaa ttcccgcgca tgaagcaggc gctgtatgtc gacagtatct
     2581 cttccgtgac cggttcgttt atcggtactt cttccgttac ggcttatatt gagtcctctt
     2641 ccggcgtatc ggttggcggt cgtaccggtc tgacggcagt ggttgttggt ctgctgttcc
     2701 tgctggttat ctttctgtcg ccgctggcgg gaatggtgcc aggctacgct gcagctggcg
     2761 cgctgattta cgttggcgtg ttgatgacct caagtcttgc tcgcgtgaac tggcaggatc
     2821 ttactgaatc tgttccggcg tttattaccg ccgtgatgat gccgttcagc ttctcgatta
     2881 ccgaaggtat tgcgctgggc tttatctcct actgcgtgat gaagattggt accggacgtc
     2941 tgcgtgacct tagtccgtgc gtaatcatcg ttgcgctgct gtttatcctg aagattgtct
     3001 ttatcgacgc tcactaaaat cgatagcgcg ttaaaaaccc gccatgcatg attgctggcg
     3061 ggttttttat ttacatctta actcgctgaa taaactcacc aaatgcggtc aattgcccag
     3121 tcaggtggtc cagcgtgccc tgatcaatca cttctccggt ttgctgatca actttgttct
     3181 gaatcacgcc gcccataaat tccggcttgt tcatcaccat tgcatcgagg aaaaccagaa
     3241 tctggcgcag gtgatactga cagcgcgcgc cgccaatcac gcccattgag ctggtctgaa
     3301 tcaacaccgg tttaccggcc agcggttgat ccggcaggcg ggaaagccag tcgatggcat
     3361 ttttcagccc acctggtacc gagtagttat attccggcgt gacgatcacc acaccgtcag
     3421 cctgacggat ctgttccgcc agagcttcaa ccgttgctgg aaaaccgtct tcctgctgta
     3481 cgtcagcgtc atacagggga atgtcggcaa tggaaggtaa cgcattgact tccatgctcg
     3541 ccggagcaat tttcggcagg gtacgtgcaa ccatgccatt aaatgagcct ttgcgcaggc
     3601 tccccagtaa cgtaaccacc tgcaattttt cagacataat gactcctgtg gttgtctatc
     3661 aatttggcga aaaagacttc atggtagaga gctgggcaaa acgcatcatc cggctggtag
     3721 gattgttggc gcggctgtga atatccggca ggcttttcca gccgtgtttg ccatctaact
     3781 cccagacact gagcttttca atcgtgggcg tgaccgccac ggaccacacc agcacgtcct
     3841 ccgcgtcgca taacgcttct acatgattta catgagcaca tttcaggcgt ccggcaatgg
     3901 gcagtagttg cagatcgcgc ggatcgtcat tcaaaacatc accggttagt tttagcacgc
     3961 ttcggcgcag cgtgatgagc tgcgcccgcg cttcgtttgg atcattttgt ttactgaccc
     4021 atagcgattc attgctggaa aatgtgtggt tatcgggcgc gtgtgggcta tgaaacccgc
     4081 gtgtcgcacg ctgcagttcc atatcgaggc cacattcacc ttcggttgtt aacgccacgc
     4141 cgaccatatt cccggcatag gaaatagaaa acgaaggcaa atttttatca ctgaaaaccg
     4201 gtttaccttt cggcagggtg acgatttccg gcaattcgcc aatgccatac agcatgaaca
     4261 tcagttctgc gagtaaacct ctggacgcca gaaaacggct ttgacgatga gggggaatgt
     4321 ttcgggcttc ttgatgacac tgggagggga gacgaacaga aatcagatgt ccttccgtta
     4381 aaatccctcg ggcaaagtga gtcgccattt ttcgctccat gcttaatggt ccattatgaa
     4441 tacaagagta atcatttact ggaatgatgg acagactaaa gcggctaaaa gttcgattca
     4501 gacaagttta ttacaaattt tttacatttt attgtgaaat agggtcgcgt ttcatacgat
     4561 tcggttatgc catcgacgcg taaagatttt taatggtttc ccgtaaccag acgatcttcg
     4621 gattatggct gttccgttta tgccacagca gggtaaaagg gacttccagc tttttttgct
     4681 ggctttcgtc aaacgggaga ggaagagcaa ccaacggcag ttggtggagt tgattgtagt
     4741 actgacagta gcgcggcgcg gtcgccagta gcagattgtc gggctgcgct gccataaaca
     4801 gtgactgctc gaattccggc aggctcatag caatcgtgcg ttcgcgcccc agctcctgta
     4861 acacattgtc cagcgcccag gtgtcgctct gttcccagca aatgctgata tgcggataac
     4921 gtaaaaaggt gtccagattc cacgtttcat gcagtgccgg atgatctttg cgtaaccaga
     4981 cgcagggcac atcactaaac aacacttcat aatcaatggc taacggtagc gagcttaata
     5041 gctcccgcga gcgaggatgg ctttcgcgac cggaaaaacc aatatccact tcaccacgag
     5101 taatggcatc taaggaatcg taatcccagt tacgtaattt gatggtcgcc tgggggtaac
     5161 gttggtagat ccgtttcgac agcgcattaa gcatgatcat catcagcggt gattccgccg
     5221 ccagttcaaa cttcaggccg cgcggtgtct ggtggtgcgg tttatccagc agcaggttgc
     5281 tcatttgcat ccattccgcc agattttgct ccatgctgac cattagcggt gtgggcgaca
     5341 gacccagcgg tgagttcaca aagagcgggt cgtcaaacca cgctcttaac tttgccagcg
     5401 acttactcac cgccgaaggt gtcacgttca tccgcttcgc cgctttggtt acgctgcgct
     5461 cctgcatcag cagttgcaga cagagcagca gattgagatc aagcgtggtg atggatttct
     5521 tcatgagcgg caaccgggcg tccaggcgcg acgaacataa tcagcaacag gctcactatg
     5581 ctacaggcaa tcagaatccc gatcagcata ttccatgcgc cgataccaac caccgctgcc
     5641 agccaaatcc acagtgacga accgcaaacc tgcgcaatac ctaaggtcga gctggctaca
     5701 cccgcgcgta atgaaaacgg ccctaacgcc tgactcatcg ccacaccaaa accaaccgag
     5761 aaaccggcgc aaatcagcgt gataccaaac agagaaaccg catgggaagg tgaaacggca
     5821 agagtgatcc ccgccgccag gaataacacc tgtgaggtga tcatcaacgt acgtggctta
     5881 aaaattccca gcgcaaatgg cgtcgagaat gaaacggtca tgctgacgcc agcggtcaac
     5941 gccataatgg tggcgtattc tccgcgttca aaacccatga tttccatcag caataccggc
     6001 gatgtattga cgaaggtgag gatcaccgaa acgctgaggg tggtgataac aacacggctg
     6061 aggaaaaacc gattaagcag cgactcgcta ttttctcgtg atttgtccga agctgcgggg
     6121 gcggcagggc gcgtttcttt taaaataaac aaagacaaca tcagtaccgc gatgcccatt
     6181 attgccatcg tccagaacag actctgccac gggaatttaa gcataatcag atgaccgagc
     6241 accggcgcta acaccggaat gatgcaggta atgccgttga gtaacgacag cactttagcc
     6301 cgacgtcgat cgtccagcgt gtcgcgcaaa atggcgaatg ccaccacgta acaacagccc
     6361 gcgcccaacc cctgtagaaa gcgccctgca agaaataacg tgctggtttc agccagtgaa
     6421 cagaacaccg aggcgataat aaatagcgcc gcgccaggga tggcgaccgg ttttctccct
     6481 gaacgatcgg ccactttacc ggcaaataac atcgcagctg ccatacctgc cagataaacg
     6541 gagaacgcaa tatgtaactg cgcttcgctg gcattgagat cggcggcgat gcgcggtaaa
     6601 ccaacgaggt acatatcaat cccagcggga taaagtaaaa ccagggcaaa actacaaatc
     6661 aaaaagcggg acatgggcta aagcgtcctg atagtaaggt gacgcaaagc atacgtggtg
     6721 aaggaggatc aggcgagttg ccatttggac ataagtgatt tcctgagagg caagaagcca
     6781 gcgaatggct ggcttcttga aggatttagc caaatttagg taacacgtta aagacgttac
     6841 cgaaccagca taaaattacg gttataccaa agagaatgac aatcgccgga ataagattgc
     6901 cgccccagac cgtgaacatc tgattgggaa actttttgcg agctttgatt gccagcaccg
     6961 cggggataat gaccgcccag atagtggcgc acagtccggc accgccgatc ccgtaaataa
     7021 agccgttcgg gaagatcaga tacaacaacg cgggtggcag gaaggttaac agcacggttt
     7081 tgaaacgccc gccgtgggag ttatcaatct taaacaggtc cgccagataa tcgaacaacc
     7141 ccagcgtgac accaaaaaac gagctggcga cagctaagtt ggagaacacc agcagacaaa
     7201 actcgataat gccgtgctgt ttggtgccga ggaacgattt caccagtgaa tcaacgttgc
     7261 cgcctgagga aataatcgcc ttaaagcttt ctcgcggaat attccccatg gtgcaataga
     7321 gccagaagag ataaatcacc agcgccagca gcgaaccgaa gaccacgctt ttgattagct
     7381 tatctttgcg tttgccatag caaataatca ggctgggaat attgccgtgg aaaccaaatg
     7441 acgccagaca caccggcaaa gccataaaga tatacgggaa gtaagacgtt cccgcagtgg
     7501 agctggtggc atcgcgcaga atggagtaat cgacctggaa gaagaaagaa ccaaacacga
     7561 tcacaaagga gataatcttc agcccgagga acaatgaggt aatacgactg gcggctaacg
     7621 agcttatcca caatacgctg gcaacgaaaa tggctgtgca gatcccgaca atacgtggat
     7681 tagcgtgata acccaaattc attgatatcg tttcactaat gatcgcacca ttagcagaga
     7741 tataggcata agtgaggata tagagaacaa aggcaacggt aataccgctg ataatgttcc
     7801 aggtgttacc aattaaatct ttggtgatgg tgttaaaact ggagccgacg ggataattta
     7861 aatttgcttc taataacaat aacccggaat gaagcattga aaaccaggca atgataagga
     7921 taaaggcacc ccagaaaaac caggcaccgg caagatcaac aggtaaagca aacatacctc
     7981 caccaattac tgtacctgct ataaccataa caccccaaaa tgcagagtgc tttttttcag
     8041 cttgatcagt catgatgcca cctttagagg aaggctattt ttgttattga ggatgtaggg
     8101 taagagagtg gctaacatcc ttatagccac tctgtagtat taattaaact tctttcagtt
     8161 ttgcggtgaa gtgacgcaat actttcggtt cgtaggtaaa ggttaaccct ttaatattcg
     8221 ccgcgttctc tttcacatgt ttgaaggctt caataatgaa gtccatatgt gtttgagtat
     8281 atgttgcgcg cggaatggtt aaacgcagta gttctgccgg gcatggcagt tgtttaccgg
     8341 ttttcggatc gcggcctaac aggaaagagc caatttctac cgcacggata ccggcgactt
     8401 tatacagctc gcaggccagc gcctgtgccg ggaactgatc tgccgggata tgcggcaaca
     8461 gtttaccggc atcaacgaat gccgcgtgac cgcccgcctg ctggcagaca acgccaatct
     8521 cttccagacc atcgaccaga tactgcacct gcgcaatacg ataagccagc cagtccagat
     8581 tcatgccgtc atacagacct acggccagac gctccatcgc gccgccttcc aggccgccat
     8641 atgtcgggaa gccttcctgt accacgcaaa gggttctgca ctcggtgtac acatcaaaga
     8701 aactgtcgtc tttcatgcac agcaggccgc ccatcggcac catcgcatct ttcttggcgg
     8761 acatcgccag catatcggca tatttgtagg tttcgcgggt gatctgctcg atggtccagt
     8821 ctttatattc tgcttcacgc tgcttgatga aataggcgtt ttcagcaaag cgtgcagagt
     8881 ccattaccac cggaatatcg tatttcttcg cgatgctgta catcgctttt aagtttgcca
     8941 gtgaaaccgg ctgaccgcct gcggagttac tggtgatggt tgcaacgata tacggcacgt
     9001 tattcggacc aacttcttca ataccgcgtt ctaatccctc aaggtcaaag ttgcctttaa
     9061 agtcgtaacg cacgcccgta tcgaaggctt ctttgatata gacgttacgc acggtacagc
     9121 cgttaatctg gctatggccc tgcgtggtat caaagaaata gttggagaac gccaccattt
     9181 tgctgcgatc caggcctttt tcctgctcgc gctttttaat cagtaccgga atatagattt
     9241 gctctgcacc acggccctgg tgagtcggaa tggtgtattg ataaccaaag atatttttca
     9301 ctgactcggc taacgcatag tagctgcggc tgccgctgta agcttcgtcg ccgcgcatca
     9361 tcgcggcctg catactctgc gtcaccgccc cggtgccgct gtcggtcagt aaatcgataa
     9421 acacatcttc gctatccagc aggaacgggt tcataccgga tttaataatt gcttcttcac
     9481 gataagcgcg agtggtacgt tttactggct caataacacg gatgcggaac ggttcaggga
     9541 gatgtttaaa gttttccatt acataatcct tcatttattt taattacagt gatccctgtg
     9601 aatattacat ttgcaatatc cattaattcg ccatgtatat atgcaagcac catcatttac
     9661 taacacaagt aaataaacga gagatggcct ttacaaaagg caaatttaag atatagcaaa
     9721 caaatagatc acattgaatc taatcggttt ggctctggtg atggctacag aagggcaaat
     9781 caagggcggt gatcgacaat cttgttgtca atattgaacc attttgaggt cacacatata
     9841 tgtaagatat tcataatgca ctt
//
LOCUS       DAZFFG010000151         9808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFFG010000151 DAZFFG010000000
VERSION     DAZFFG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.151"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..222
                     /locus_tag="ACHJ7J_004357"
     CDS             <1..222
                     /locus_tag="ACHJ7J_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5002504.1"
                     /translation="TSQRCACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAA
                     GHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            266..373
                     /locus_tag="ACHJ7J_004358"
                     /pseudo
     CDS             266..373
                     /locus_tag="ACHJ7J_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(357..995)
                     /gene="ytfB"
                     /locus_tag="ACHJ7J_004359"
     CDS             complement(357..995)
                     /gene="ytfB"
                     /locus_tag="ACHJ7J_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO5002505.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1214..1834
                     /gene="fklB"
                     /locus_tag="ACHJ7J_004360"
     CDS             1214..1834
                     /gene="fklB"
                     /locus_tag="ACHJ7J_004360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5002506.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2143..3555
                     /gene="cycA"
                     /locus_tag="ACHJ7J_004361"
                     /gene_synonym="dagA"
     CDS             2143..3555
                     /gene="cycA"
                     /locus_tag="ACHJ7J_004361"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO5002507.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3600..4262)
                     /gene="ytfE"
                     /locus_tag="ACHJ7J_004362"
     CDS             complement(3600..4262)
                     /gene="ytfE"
                     /locus_tag="ACHJ7J_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO5002508.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4370..5335)
                     /gene="ytfF"
                     /locus_tag="ACHJ7J_004363"
     CDS             complement(4370..5335)
                     /gene="ytfF"
                     /locus_tag="ACHJ7J_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5002509.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5443..6303)
                     /gene="qorB"
                     /locus_tag="ACHJ7J_004364"
     CDS             complement(5443..6303)
                     /gene="qorB"
                     /locus_tag="ACHJ7J_004364"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5002510.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALSAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            6392..6772
                     /gene="ytfH"
                     /locus_tag="ACHJ7J_004365"
     CDS             6392..6772
                     /gene="ytfH"
                     /locus_tag="ACHJ7J_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO5002511.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6901..8844)
                     /gene="cpdB"
                     /locus_tag="ACHJ7J_004366"
     CDS             complement(6901..8844)
                     /gene="cpdB"
                     /locus_tag="ACHJ7J_004366"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO5002512.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKTNLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQTKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9034..9774
                     /gene="cysQ"
                     /locus_tag="ACHJ7J_004367"
     CDS             9034..9774
                     /gene="cysQ"
                     /locus_tag="ACHJ7J_004367"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO5002513.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2243 a         2507 c         2586 g         2472 t
ORIGIN      
        1 acaagccagc gttgcgcgtg ctgtggtcat acagcgaaag aaaatcgcct gtcacaaagt
       61 aaattcagat gccaggcatg tggatataca gcgaacgctg atgtaaatgg cgctcgtaac
      121 attttagcgg cggggcacgc cgttcttgcc tgtggagaga tggtgcagtc aggccgcccg
      181 ttgaagcagg aacccaccga aatgattcag gcgacagcct gaacgtagca gggatccacg
      241 tccttcaggg cgtggaggat gtcaacacat cagtatggtt tcaccccagg cactggaagc
      301 cagacgtaag gtcatcaaag agacaatcct tagctggctt ctggttgacc cttcatctac
      361 cgcgcgcgaa taaaactgcc atccggctgg cgggtaaaca ggacctgttg attattcccc
      421 gtatcaatgg ttaagcctgt caccacgccg ctggcgttct ggcggatttt caccatctgc
      481 ccattttgca aattactcag cggtttaccc gcgccttcta cctgcgccat tgcatagaca
      541 tcggtagcgg gcagtccgtg gtcgcggaac agctgcgcca tcgttttacc gggttcgaca
      601 cggtaagagc gccattgatt atcaattccg ctatctggct ggaatggttg tgtttgggta
      661 gtttgcggct gctcttctgg ctggccttcc tgtataggtt ctggagcgac cggagccacc
      721 tgatcaggat cattttgtgg tgtgactaat tgcgcccgca gctgttcttc tgtcggcggt
      781 tgtgattgtg actgaatatc taactgcgct tcacgcgtca ccaccggcgc gttgggcgta
      841 tcatcagatg gaagcagaaa tccgaccacc aatacaatgg cggcaataat gatcccccga
      901 cgatgcatcg gcggcagcgg gtccatgaag cgaaaattgt ccggcgcgtg ccagactttc
      961 tccagggttg gttttagttc aaagcgcccg ggcatggttt cctcctgctc cgcgtcttgt
     1021 tcctcaatca tagcctatga ataagctaac gctatgatgt ccgtggtaaa cccgccttta
     1081 ttatattcat acgggatatt gctattgttt ctttttcccc gggatttgtc atcattcctg
     1141 cgacaaagtt ttacccaaag aagtgtggct gatatgctgc ccgctacttt tataccctaa
     1201 gaaaggaaat acgatgacca ccccaacttt tgacaccatc gaagcgcaag caagctacgg
     1261 cattggtttg caggtagggc aacaactgag tgaatctggc ctggaagggc tgctgccaga
     1321 agcactggtt gcaggtattg ccgatgcgct ggaaggcaaa catccggctg ttccggttga
     1381 tgtggtgcat cgcgcactgc gtgaaatcca cgaacgcgcc gatgccgttc gtcgtcagcg
     1441 tttccaggcg atggctgctg aaggtgtgaa atacctggaa gaaaacgcca aaaaagaagg
     1501 tgtgaatagc accgaatctg gcctgcaatt ccgcgtgatc aaccagggtg aaggcgcaat
     1561 tccggcacgt accgaccgcg ttcgtgttca ttacaccggt aaactgatcg acggcaccgt
     1621 gtttgacagc tccgttgctc gtggtgaacc cgctgaattc ccggttaatg gcgtgatccc
     1681 tggctggatt gaagcactga ctctgatgcc ggtaggttct aaatgggaac tgactatccc
     1741 gcaggaactg gcatatggcg agcgtggcgc aggcgcgtcc atccctccgt tcagcaccct
     1801 ggtgtttgaa gtcgaactgc tggaaatcct ctaagcagcg cattctgttc ccctcgagcc
     1861 agaggggagc aggcattcag caataacccc ttcagtttgc caaacggcgc tattttgtgt
     1921 tgcaaagacc ccgtaagcgt gtatttttgt gagctgtttc gcgttatcac cgtgatatga
     1981 cactcacttt aaacataaaa ttaacattag atctaaatct tagtattcat cccgcgtatt
     2041 gttacctaat atcgatgagt cccgatacag attcgtcgta tcatagactg actaaaggcc
     2101 gtagagcctg aacaacacag acaggtacag gaagaaaaaa acatggtaga tcaggtaaaa
     2161 gtcgttgccg atgatcaggc tccggctgaa cagtcgctac ggcgcaatct cacaaaccga
     2221 catattcagc ttattgccat tggcggtgcc attggtacgg ggttgtttat ggggtctggc
     2281 aaaacgatta gccttgccgg gccgtcgatc attttcgttt atatgatcat tggttttatg
     2341 ctctttttcg tgatgcgggc aatgggggaa ttgctgcttt cgaatctgga atacaaatct
     2401 tttagtgact tcgcttccga tttactcggg ccgtgggcag gatatttcac cggctggact
     2461 tactggttct gctgggttgt aaccggtatg gcagacgtgg tggcgatcac ggcttatgct
     2521 cagttctggt tccccgatct ctccgactgg gtcgcctcgc tggcggtgat agtgctgctg
     2581 ctgacgctta acctcgccac cgtgaaaatg ttcggtgaga tggagttctg gtttgcgatg
     2641 atcaaaatcg tcgccattgt gtcgctgatt gtcgtcggcc tggtcatggt ggcgatgcac
     2701 tttcagtcac cgactggtgt ggaagcgtca ttcgcgcatt tgtggaatga cggcggctgg
     2761 ttcccgaaag gtttaagtgg cttctttgcc ggattccaga tagcggtttt cgctttcgtg
     2821 gggattgagc tggtaggtac aacagctgcg gaaaccaaag atccagagaa atcactgcca
     2881 cgcgcgatta actccattcc gatccgtatc attatgttct acgtcttcgc gctgattgtg
     2941 attatgtccg tgacgccgtg gagttcggta gtcccggaga aaagcccgtt tgttgaactg
     3001 ttcgtgttgg tagggctgcc tgctgccgca agcgtgatca actttgtggt gctgacctct
     3061 gcggcgtctt ccgctaacag cggcgtcttc tctaccagcc gtatgctgtt tggtctggcg
     3121 caggaaggtg tggcaccgaa agcgttcgct aaactttcta agcgcgcagt accagcgaaa
     3181 gggctgacct tctcctgcat ctgcctgctc ggcggcgtgg tgatgctgta tgtgaatccc
     3241 agcgtgattg gcgcgttcac gatgatcacg accgtttccg cgattctgtt tatgttcgtc
     3301 tggacgatta tcctttgctc gtaccttgtg tatcgcaaac agcgtcctca tctacatgag
     3361 aagtcgatct acaagatgcc gctcggcaag ctgatgtgct gggtatgtat ggcgttcttt
     3421 gtgttcgtgg tcgtgttgct gacactggaa gatgacactc gccaggcgct gttggtcacc
     3481 ccgctgtggt ttatcgcgct ggggctgggc tggttgttta ttggtaagaa gcgggctgct
     3541 gaactgcgga aataaccgca ttatcatgct ggatggcgca atgccatcca gcttttagat
     3601 cactcacccg ccagcgcgcg tgggaacagc acattatttt ccaggctgat gtgatccatc
     3661 aggtcatcaa tcagttcatt aatgccgtta tacatcgctt tccaggtggt gcaggcttcc
     3721 ggcggcggtg tgacgttatt ggtagtgtgt ttgatcactt ccagcagttc acccgcttca
     3781 tcgtgctcgc tttccattac gctgattggc cccattgcct ggctgcccat gccttgtttg
     3841 atcatcggga agagaatctg ctcttctttc atcatgtggc tggaaagctc ttcatgcagc
     3901 atagtcaggt attttgtcag cccttttggc acgctcggtt tgtcggcgtg aacgcgctcg
     3961 actttagtcg cttgcaggat cagctccggc agttgctcgc ggtgacgatc gtggtagcgc
     4021 acgatgatat ggtcaatgat ttctgccagc ggggcgctac gccagtcttt ctcaatcggt
     4081 tgttcagcaa gctttgccag ttcagcttca atgacctcaa catccagttc tttacgtgcc
     4141 gccgcgcgcg ccagcgtctg cttaccgcca cagcagtaat ccatatcata tttacgaaac
     4201 agagctgaag cgcgaggaat agagagcgcc agttcaccta aaggttggtc gcgataagcc
     4261 atagctgata cctcattctt aataagataa gatgtatttt aaatgcatct ttaagacaaa
     4321 tgctataacc cttacgtagc agaggcttat ttaactcact gccagcatgt caactttctg
     4381 agacagattc agttaaaggt ttttctggtt ttgctctgac cgcaatgacc acgccaatca
     4441 ctaacagcgc gataccgctc agcgtcatta gcggcggcat ttgctggcgg agtaaaaagg
     4501 tgtacagcaa acctgccagc gtttcgaaaa caatcagcgg cccgagaatc actgtcggta
     4561 atcgctggct ggcgacgttc cagcagagtg cgccaaccca tgagcaaagc acggctatcg
     4621 caaccatcag actaataaac accagcggac gggggccaaa gggtagggag aagtccggcg
     4681 tttgcgtatt cagccagtaa caagcgacga gatagccgat aagtgaaacc ggcagtgtga
     4741 ccagcgcctg cgctgttgcc cacatcattg gatgtttgtc gggattttcc cgcagccagc
     4801 gggcgttgcg cagggcatac cacgcccagc agaccacgga aactaacgcc agcacgatgc
     4861 cagaggtata acgtgcccag tcaaaatcgg ggagtccgtg gtttaactca gcaatattca
     4921 cactcgcgag gccgatgcca atacaaatca gtgccggggc gagttttccc cacgcgagtt
     4981 tgccgtcgcg ctggctataa agcagattgg caaagacagg aatgaccacc ggcagggtgc
     5041 cgataatcat cgtggaaaca ggcgcgccag tacgttgaat ggcactggca aggcagaagt
     5101 aataaatgag gttgcccatc atagtgagca tcaaggcggt aagccagtcg cgacgcgcca
     5161 actgacgcag acgcacgcgt cccagccagg caatgggcag cgcaattaac cctaacgcca
     5221 gataacgccc catcgactgc aacatcgccg ggtattccgg cacgatcaac ggcccgacaa
     5281 aaataagccc ccacatcaac cctgctaaca gggcgtacag cacgccgcta atcattactg
     5341 gcatccattg atctgtcaga agaaagttca ggaagcagac atgattgctc ccggcatccc
     5401 gtcattatca gagaggatgc gggaggatgc cactttaatt aactagttat taacattaaa
     5461 aagatggctt acgctttcgg ctaacgttgt cgttgggtgg ccaatcaatt tgctaagtgt
     5521 tttgctgtca tcaaacagac cgcctttcga tgcgccaacg tcagaatccg ccagcatatc
     5581 cgccagtccg tcgggcagtc cgacgctttt cagtgccgcg gcgaaatcgg cttcgctcag
     5641 attttgatag gtaacctgtt tgccgctctg tttggtcagc tctgccgcta actgtgtcaa
     5701 cgtccaggca ctatcgcccg ccagttcgta aaccttgcct tcgtgaccgg cttcgctaat
     5761 cacgcgtgcc gcagctgccg cataatctgc ccgcgttgct gaggcgattt tgccatcgcc
     5821 cgccgcaccg ataaatacgc cgtgttccag tgctgccggg gcgctggcga ggtagttttc
     5881 gctgtaccag ccgttgcgca gcagggtgta aacgatgcca gaatcagcca acattttctc
     5941 cgtctcgatg tgctcatcgg cgaggccgag cggggaggta tctgcatgta gcaggctggt
     6001 ataagcgata aatttcacgc cagccgcctt tgcggcatta ataacattac gatgctgcgg
     6061 ggcacgttga cccacttcgc tggaagagat cagcagtagt ttttccactc cctgaagtgc
     6121 agatgtcagt gcggcttcat cgccgtagtc agcctgacgc acggtaatgc cttgtgctga
     6181 cagggcctgg gcttttgccg gattacgaac gatagccact atttggctgg caggaaccgt
     6241 tttcatcaag gattcaataa catagtgacc aagttggcca gtggcaccag taatagcgat
     6301 catgggaagt ctccattgtt ttgtcttgtg tttaaatacg ctaacaccta aacttacttt
     6361 tagtaagtac atacaaaaag gtaagtatga aatgagtcag gttagcctgt cgcaacaact
     6421 gaaagagggt aatctctttg cggaacagtg cccgtcgcgc gaggtgttga aacacgtcac
     6481 cagccgttgg ggggtgttga ttctggtggc gctacgcgaa ggtactcatc gctttagcga
     6541 cctgcggcgc aaaatcggcg gggtgagcga aaagatgctt gcgcagtcgt tacaggcgtt
     6601 agaacaggat ggttttctta accgtatcgc gtatccggtg gtgccaccgc atgttgaata
     6661 cagcctcacg ccgctgggcg agcaggtgag cgaaaaggtt gctgcactgg cggactggat
     6721 tgagttgaat ttgcccgagg tgttggcggt gcgggatgaa cgtgcggcat aactgccgga
     6781 tgcgctgagc ttatctggtc tacggtgcat ggcttgtagg gcggataaga tgcgccagca
     6841 tcgcatccgc caataaatgc cggatgcggt tgatgattta taggccgaaa aagaagtgtt
     6901 ttacttactc aaatccacct gataaatcgc aaacccgata tcatcggtcg cgactttatt
     6961 catcggatac tgccctttct ctttaataaa cgctgcggct ttatctgacg gagaggtttc
     7021 gaaacggata tccagtttct tatcgccagc tatcggtgct aaacgccagt tgttatctgc
     7081 cgccgggtga atttcccccg cacgtttcga ctcatcagca atccacgctg ccagcaccga
     7141 gcggttctca tccggtgaag caaaagcgat atggctgtcg cccgtaccgg caaatttgcc
     7201 gccgtaagcg cgatagttat tggtggcaac gaggaacatg gcgttcggat caatcggctt
     7261 gccattaaag gtcaggttct taatcctttc cgcattggca ttaatcatct ggcactcgcc
     7321 gtcataacgg gcgggctggg taacatcaat ctgataattc acaccatcaa taacatcaaa
     7381 gttataagtg cggaaaccat cccagttgat gagtgactgt ggtttcgtgc tgttgggatc
     7441 aatctggtta aactgtcccg cggagcactc cagccactct ttcacctctt taccgctggc
     7501 tttcaccaca atcagcgtat tgggatagag ataaagatcg gcggcattac ggaaggtcaa
     7561 ctggcctttt tccacctcca catagcttgc cgggtcattt ttgcgaccac cgactttaaa
     7621 cggtgcggca gctgaaagca ccggcagttt tgccagatcc ggatcgccct gaatgtaatg
     7681 ttcgacatac gctttttgcg cgttgttcac tacttgcacg gtcggatcgt cctgcaccag
     7741 cgccagatag ctatacatat tgtcggcaga tttaccgatt ggcttgctga cgaactggcg
     7801 tgtggcatcg tgatcggctt tgagtgtttc taccagcttg ctgtcttccg ccgcgaggga
     7861 ttttttattg gcgatgtcgt aaatcggccg tgcttccgct ttcgtctgcg tcacctgcca
     7921 tttaccgctg tcattactga gttgtaagtc gaccacccca agatgatcgc cccacatgcc
     7981 cggcattacc gccggaacac cattcagcgt gcctttggtg atatcagccc cttcgatatc
     8041 agcaaaatct ttacccggga aaacggcgtg agcatggcca aacataatgg cgttaacgcc
     8101 cggaatttca ctgaggtaat aaactgagtt ttccgccatt actttatacg gatcggcaga
     8161 cagcccggaa tgcgccagaa cgacaacgac atcggcacct ttctcgcgca tttcaggcac
     8221 gtatttgcgc acggtttcgg taatatcatt caccgtcact ttcccggata aattagtttt
     8281 atcccagccc atgatttgtg gcggcacgac gccaatatag ccaatcttca gcgtctgttt
     8341 ttttccgtct ttatcgacca cttcggtgtc tttaattaaa tacggtgtaa acattggctg
     8401 tttggttctg gcgtcaatga cgttggcatt tacataaggg aatttcgctc ctgccagcgc
     8461 atttttcagg taatccagac cgtagttaaa ctcgtggttg ccaagcgttc cgacggtata
     8521 gtccagcgta tttaatgcct tatagaccgg gtgaatatca cctgctttta atcctttcgc
     8581 cgacatgtaa tcggccagcg gactcccctg aatcaaatcg ccgttatcaa ccagtacgct
     8641 gtttttcact tcattgcggg catcgttaat caggcttgcc gtacgtacca gtccgaattt
     8701 ttccgtggcg gtgtctttgt aataatcgaa atccatcatg ttgctatgca gatcagtggt
     8761 ttccatgata cgcagatcga ccgtcgctgc attcacactg gcggcaatca gcgtggccag
     8821 gagcgttgcg ctaaacttaa tcatcaggga catcctttta tcatcgggaa tacgaaagaa
     8881 aagggataat aaacgcctta cttatagaac agtgaagaat gccacaattt tacgctttga
     8941 aaatgatgac actatcacag ttggcgcatt cattaacgat agggtataag taaaacaata
     9001 agttaacacc gctcacagag acgaggtgga gaaatgttag atcaagtatg ccagcttgca
     9061 cggaatgcag gcgatgccat tatgcaggtc tacgacggga cgaaaccgat ggacgtcgtc
     9121 agcaaagcgg acaattcccc ggtaacggca gcggatattg ccgcacacac cgttatcatg
     9181 gacggtttac gtacgctgac accggatatt ccggtccttt ctgaagaaga tcctcccggt
     9241 tgggaagtcc gtcagcactg gcagcgttac tggctggtag acccactgga tggtactaaa
     9301 gagtttatta aacgtaatgg cgaattcacc gttaacattg cgctcattga ccatggcaaa
     9361 ccgattttag gcgtggtgta tgcgccggta atgaacgtaa tgtacagcgc ggcagaaggc
     9421 aaggcctgga aagaagagtg cggtgtgcgc aagcagattc aggtccgcga tgcgcgcccg
     9481 ccactggtgg tgatcagccg ttcccatgca gatgcggagc tgaaagagta tctgcaacag
     9541 cttggcgaac atcagaccac gtccatcggc tcttcgctga aattctgcct ggtggcggaa
     9601 ggacaggcgc agctgtaccc gcgcttcgga ccaacgaata tttgggacac cgccgctgga
     9661 catgctgtgg ctgcagctgc cggagcgcac gttcacgact ggcagggtaa accgctggat
     9721 tacactccgc gcgagtcgtt cctgaatccg gggttcagag tgtctattta ctaaattcag
     9781 atggcagaaa cagtgtattt cctgattc
//
LOCUS       DAZFFG010000152         9650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFFG010000152 DAZFFG010000000
VERSION     DAZFFG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.152"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(573..1220)
                     /locus_tag="ACHJ7J_004368"
     CDS             complement(573..1220)
                     /locus_tag="ACHJ7J_004368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002514.1"
                     /translation="MNRSNEEDIEKAKSLISIGAEIAGAAVGGAIGFFATGPVGAGSL
                     GVIVAKAGAKLLGDVADRTMSAREKVRVGAAAAVAFNKIEKVALSGNEPRKDDFFNSE
                     EGKRSDAEEILEGTLRRAREEYEEKKILLLGNFYANMVYSPGVSTEEANYYLRLFDSL
                     TYRQLCVMSLIMMKPQYSDFQNLRSHDYRTQNAKMPKCQYASKYCSTPPRNLCSV"
     gene            complement(1905..2840)
                     /locus_tag="ACHJ7J_004369"
     CDS             complement(1905..2840)
                     /locus_tag="ACHJ7J_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002515.1"
                     /translation="MSFLKTLMDSLSAISGSVANASETEKKKDRRSDEEFTDFKDDSS
                     LINYLTKQYIDNELAVDKSKNHWEVFSQHLKPLIHCLKRYEDASLSQRETSQKEQAAA
                     MLEALQQGKELSEHSGNGTIIGRDDYVNGLKKITLSYCQKHVLTMVDFLDNVPDHLCD
                     GKNASTSWQSFRKDASKKRWKNFDQACRDNSLYFSVGKVLFDTTLWGSGKHGIMLGRD
                     DITCLGGEPDKFRVLWSNVTSLWHHKGYLYVNDYKTGFVANDDACKLLELLEEHFDKM
                     KRSEGNLLLAYLGYDRSGQQAIKNAYEADVQQFIE"
     gene            complement(2837..5149)
                     /locus_tag="ACHJ7J_004370"
     CDS             complement(2837..5149)
                     /locus_tag="ACHJ7J_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO5002516.1"
                     /translation="MNDKLKMWRDKAGLSQQEVAERAGENVLSQKQVSRIEQAPLSQN
                     LELVLAYLRIVAPEKIQEFITDCLKTQLNTSSLNQNYTQQKENMMRNKKELIEYLERS
                     IATLSNTSNPFIKNSSITKDIAELKDNLKDIHAKPVLSAMGPSDAGKSTLLNYILGSE
                     ILPAKWQPATCLVTLLMHTQDKPDYLDKNKSVFIFKNGFNPHMIFDRQMLADFLIDSG
                     GKELIKTYGEKDENDNVLNPQAYMVVVFESFAILETIWLLDTPGQLIEPEMYDEIDGC
                     SSEEILNDKAKAMSAISLVDGLIFASATTKFMRDEDPEFLQNILMHKVPLEGDKPLAN
                     LIILKTHSYDQITAEANHKTFRTAANHLTKTFSHTLYDEWEHQLNNPNHPQKLLRPDA
                     AVWASRMLPFFRENRQYCEAFDVRLKEVINYLLANRTALINNRIETIHKRIQNRIALA
                     LQELQQSSRSVQERKEDIEQQDARFREGSGALIARFTEISNFCPRYAEEDIERIRDIF
                     YSLCNDESMYRFIDNRFEDKDEAKRAINKALGNHFESKFRRVMEESTRRFSLEVESVV
                     QAWQKIVPSAQGAARAGMPETDISLSATSDFDAYSAFVGGMAGLTSFGAMAGYVATIS
                     SNLGAYILVGKAAGVLTSLGITSSVTTLPTLVAATGGPVAWGVAIAAVVGLLVYRLFA
                     NWKKALAKSVVKGLESSNTLSNVEADITRYWQDSRNALRASVDSLKNETDAFIRHLYA
                     EANIEYQQQDIAEAEQILNTIKNNLGSEDK"
     gene            complement(5454..6056)
                     /locus_tag="ACHJ7J_004371"
     CDS             complement(5454..6056)
                     /locus_tag="ACHJ7J_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5002517.1"
                     /translation="MNTSPWNKDRIIGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGNSVSSRATVLQQKTGSPVQFEITKGTREAVSALIKL
                     GNLRSKDYLFRSRVGTNQHISTRQYNRIFHGWVAKLGLEDSLYSTHSLRRTKPYLIYK
                     KTKNLRVIQLLLGHNKLESTVRYLGIEVDDALEISESIEV"
     gene            6455..7555
                     /locus_tag="ACHJ7J_004372"
                     /pseudo
     CDS             6455..7555
                     /locus_tag="ACHJ7J_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hsdR"
     gene            complement(7789..9582)
                     /locus_tag="ACHJ7J_004373"
     CDS             complement(7789..9582)
                     /locus_tag="ACHJ7J_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO5002518.1"
                     /translation="MATLQQELIVRGEQVERVFINYQDKKYVVNRQYQRKLIWTIEEK
                     QNFIDSIIKGFPIPIILLAEPLGRKDGTLEIIDGMQRMNAIMSFISNDFSFNGFYFDL
                     NTFATTKDLLDRGVIDQKMPLLDRSQCLAIASYPVPLSIYEAAERDSVEEVFRRINSG
                     GRQLSRQELRAAGATNVFAECVRKISARVRGDTSNADLLLLSEMKKISITNKDLDYGI
                     SADSVFWIVNGILTKEQLRQSRDEEIVADILAYMVSDKPLASRTELLDDYYGAISPEA
                     LASKARFEAIDQAIKKRTGELVDLDYQRVHDAIILLLSQAQTTFSGLIFPNGNGGNPV
                     PRYFQVVFLAMHDLIIKRGMVVSDSAGLIGRLKDCGRNIQVQDGGRWGADNRSRMVDS
                     IIGWIQGFFENDQNPDPAKVHWITKLQNLLTNSITEQAAYDFKQGFYTLDPSPSFDER
                     SFEKIMETCAAIANIGRGHKGYVLVGVAENAHTATRVQEIFGSKALSYNGFHITGVDH
                     EANHAGKNLDQMFQDISDRVGRSGLSEPLKSYINTHLKCVRYFDKTVFVFEILGQDTP
                     SHIAGKWVERQGAQVRDIAPEGMVALFERFR"
BASE COUNT         2547 a         2207 c         2082 g         2814 t
ORIGIN      
        1 taaaattatc catgtttgat gagtgaaaat aatgttagga aatgaacctg tttaaaaact
       61 cgttatcata gattaaccgt tcagatgctc aacgaactca ataaattggc tgagtctggt
      121 gtattaactc agacctgatc tgacagttac cggttatgta aacatttatc gttgctgcca
      181 atgcttggat tattgtcctc aaggtattcg ctttacttac aagctaattt tgaaacagat
      241 gtcgcgctat tttaggaaga gaatctatgc aaaagtttgc tatgagcgag gagcggatgt
      301 ttatgattgt tatcaacgag gccccgtgtc taacaatctc ctgtgttaat aagcaggggc
      361 tggaaacttt gaatcaaaat gttcattctt aacgcaaagc ttctgcgata gtagtaatat
      421 cgggaagtga taaacttcct ccccccataa aaagtttgta aaacctttca cctaaccctg
      481 taaattctaa tcctaaaggg atgatatcat gccatgccag caaagataca taaccatttc
      541 cgccagcact cctaacgcag actaatccca aattatacag agcataaatt tcttggagga
      601 gtgctacagt acttgcttgc atattggcat tttggcattt tggcattttg ggttcgatag
      661 tcatggcttc tcagattttg aaagtcagaa tattgtggtt tcatcatgat caaactcata
      721 acacagagct gccgatacgt cagtgaatcg aacaaccgga gatagtaatt cgcttcttct
      781 gtactcactc ccggagagta gaccatattt gcataaaaat tccctagaag aagaatcttc
      841 ttttcctcat actcttcacg cgcccttctt aacgtcccct ccagaatttc ttctgcatca
      901 ctgcgttttc cttcttcaga gttaaagaaa tcatcttttc tgggttcatt acctgacaga
      961 gcgacctttt caattttatt gaatgcaact gcagcagcag caccaacgcg gactttttcg
     1021 cgtgcagaca tcgtacgatc agccacatca ccgagaagtt ttgcaccagc tttcgcgaca
     1081 atcaccccta acgaaccagc ccccacaggg ccagttgcaa aaaagccaat tgcaccgcca
     1141 actgcagcgc cagctatctc tgcacctatc gaaatcagcg atttggcttt ttcaatatct
     1201 tcttcgtttg atctattcat agctttatct tctaggacat aataatggat ggttgtaatg
     1261 aagatacctt taccacatga aaatcattaa ttccacatta cctgatgaca gtgaactgtt
     1321 gtccgttgat taacgcgcaa taatgctatt caaatggatg tttgaccacc tggaactaac
     1381 cgctgtaatg acatctattt tgggtacaga ggagaccaac cggcgtgtcc tcaggtccga
     1441 tatgagcgat aagcggaagt tcaaaatact tgttattggc ttttcgaaga tcttgctgta
     1501 gtaatcaaaa aggagcaggt agtgttatta acgggatagc tcccgaggat attatgcatc
     1561 atcacggcag cctgctttca tattttttga tcttaataaa atttatctgc gtattaaact
     1621 accggatttt aggttttcca aaaatgatag tattaccagt ggactcacaa ccttggcatt
     1681 cctgattcta cttaaacagc cgtatccttt tgcgcacata ttcgagcgcc agtgtgacga
     1741 gttttgatca cgcattcctc tgctcactgt tttaacatta acaggatttc aaagtatcct
     1801 gaattactga cccactatgc cggttggtat tgatgttgac agcgtgacgg gcactcaggc
     1861 ccgccacagt atggtatctt aaataccgcg tcagtcgcag tatgttattc aataaactgc
     1921 tgcacatccg cttcgtaagc gtttttaata gcttgctggc cggagcggtc gtaacccaga
     1981 tacgctaata acaaatttcc ttcggaacgt ttcattttat cgaaatgctc ttcgagcagt
     2041 tccagtaatt tacaggcgtc atcattggcg acaaaccccg ttttataatc attcacgtaa
     2101 aggtagcctt tgtgatgcca caagcttgtt acgttgctcc ataaaacacg gaacttgtca
     2161 ggctccccgc ctaagcaggt aatatcatcg cgccctaaca taatcccatg cttccctgag
     2221 ccccacagcg tggtatcaaa caagacctta cccacagaaa aataaagcga attgtcccgg
     2281 cacgcctgat caaagttttt ccagcgcttt ttactggcat cttttctaaa cgattgccat
     2341 gaggtactgg cattttttcc gtcgcacagg tgatccggaa cgttatccag gaagtcgacc
     2401 atagtaagta cgtgcttctg gcaatagcta agcgtgattt ttttcaggcc gttaacatag
     2461 tcatctcttc caatgattgt gccgttgccg ctgtgctccg agagttcttt accctgctgt
     2521 agggcttcca gcatagctgc cgcctgctct ttttgtgagg tttcacgctg gctgaggctg
     2581 gcatcttcat agcgtttcag gcagtgtatg agcggcttca ggtgctggct gaaaacctcc
     2641 caatgatttt tgcttttatc gactgcgagt tcgttgtcga tatattgttt tgtcaggtag
     2701 ttaataaggc tgctgtcgtc tttgaaatcg gtaaattctt catcactgcg acggtccttt
     2761 tttttctctg tctccgaggc attggctaca gaacctgaga tcgcgcttaa ggaatccatt
     2821 agtgttttaa gaaaactcat ttatcctcac tacccagatt atttttgatt gtatttaaaa
     2881 tttgttcagc ttcagcaata tcctgctgct gatattcgat attggcttct gcatacagat
     2941 gcctaataaa ggcatctgtc tcatttttca gtgaatcaac cgacgctctc agcgcgttgc
     3001 gtgaatcctg ccagtagcgc gtgatatccg cttccacatt gcttaacgta ttactggatt
     3061 ccagcccctt aacaactgat ttggcgagtg cctttttcca gtttgcgaac agtcgataga
     3121 ccagtagccc tacaaccgcg gcaatagcta cgccccatgc caccgggcca ccagtggcgg
     3181 caacgagagt tggtaatgtc gtgaccgaac tggtaatccc tagggaagtc agcacgccag
     3241 ccgctttgcc aactaaaata taggcaccta aattgctcga tatagttgcc acgtaacccg
     3301 ccatagcgcc gaagctggtt aatccggcca tacctccgac gaaggcggag tatgcatcaa
     3361 agtcggaggt ggcggaaagg gaaatatcag tctccggcat tcccgcacgg gctgcgcctt
     3421 gggcggaagg cacgattttc tgccaggcct ggaccacgct ttccacctca aggctgaacc
     3481 tacgggtgct ctcttccatt acgcgacgaa atttgctttc gaaatgatta cccagcgctt
     3541 tattaatagc ccgtttcgct tcatctttat cttcaaagcg attgtcgatg aaacggtaca
     3601 tactctcatc attgcaaagc gagtagaaaa tatcgcggat gcgctcaata tcttcctcgg
     3661 cgtagcgcgg acagaagtta ctgatttcag taaagcgagc aattaacgcg ccggaaccct
     3721 cgcgaaatcg cgcatcctgt tgctcaatat cctcctttct ttcctgaaca gaacggctac
     3781 tctgctgtaa ttcctgcagc gctaatgcaa tcctgttttg gatgcgttta tggatggtct
     3841 ctatccggtt gttaatcagc gcagttcgat tggcaaggag ataattgatc acctctttca
     3901 ggcggacatc aaaggcttcg caatattgtc tattttcccg gaagaatggc agcattctgc
     3961 tggcccagac agcggcgtcg ggccgtagta atttctgtgg atggttggga ttatttaact
     4021 gatgctccca ttcatcatac agggtatgcg aaaaggtttt cgtcaggtga ttcgcggcgg
     4081 tacgaaacgt tttatggttc gcttcggcgg taatttgatc gtaactgtgg gtttttaaga
     4141 tgatcaaatt cgccagcggc ttgtctcctt ccagaggcac cttatgcatt aaaatatttt
     4201 gtaagaactc cggatcttcg tcacgcataa atttggtggt agcagatgcg aagataaggc
     4261 catctaccag cgaaattgcg ctcatggctt tggctttatc gttaaggatt tcttcgctgg
     4321 agcatccatc aatttcatcg tacatttcgg gttcaattaa ttgccccggc gtatcaagta
     4381 accagatagt ttccagaatc gcgaaacttt caaacaccac aaccatataa gcttgtggat
     4441 taaggacatt atcgttttca tccttttcgc cataggtttt gatgagctct tttcctcctg
     4501 aatcgatcag gaagtctgcc agcatttgac gatcaaaaat catatgcgga ttgaaaccat
     4561 ttttgaaaat aaagactgat ttattcttat ccagataatc aggtttatcc tgagtgtgca
     4621 tcagtagcgt gacaaggcag gtcgcgggtt gccatttcgc cgggagaatt tcgctgccca
     4681 aaatatagtt taacagagta gactttcctg catctgaagg ccccatagcg ctcaggacgg
     4741 gcttggcgtg aatatcttta agattgtctt taagctcggc gatatccttg gtgatgctgc
     4801 tgtttttaat aaagggatta gaagtattgg acagcgtggc tatcgatctt tccaggtact
     4861 ctatcagttc ctttttattt ctcatcatat tctctttttg ctgtgtgtag ttttgattta
     4921 aagaggacgt gtttaactgc gtctttaaac aatcagtgat gaattcctga atcttttcag
     4981 gcgcaacgat gcgaaggtag gccagcacca gttctaaatt ttgtgacagc ggtgcctgtt
     5041 caatacgcga gacctgcttt tgggacagaa cattttcacc tgcccgctcg gcgacttcct
     5101 gttggcttaa acccgcctta tctcgccaca ttttcagttt atcgttcatt acaatgccca
     5161 ccatcttcag accggtatgt ctaaaatagc aatgaatgca tgatgaatcc agacgtattt
     5221 gtctaaaaaa aagaacaatc cgttagatca taatcgatat ccctagtaac tacctgatat
     5281 taaagatttg ttaatataaa atgccgaatg gacgaaactt attagtgata agggttatcc
     5341 gtagtattga aaaattcgtg atattaatgt gatctgttta ccataaatta acatgattca
     5401 gtgccagcaa tgtccgcttc tggcacagga ctgctgttgc agccctggca accttagact
     5461 tcaatcgatt cagagatctc taacgcatca tcgacttcaa tgcccagata acggactgtg
     5521 ctttccagtt tattatggcc taacagaagt tggatcaccc tgagattctt agttttcttg
     5581 taaatcaggt aaggttttgt tcttctcaag gaatgtgtac tgtaaagcga atcttcgaga
     5641 ccaagttttg ctacccaccc atgaaaaatt cggttgtatt gccgggttga tatgtgttgg
     5701 ttagtaccga cccgagatcg gaacaaatag tctttactgc gtaaattgcc aagctttatc
     5761 aatgcagaaa cagcttctct tgtccctttg gttatctcaa attggacagg gctgccggtt
     5821 ttctgttgta gaaccgtagc tctgcttgaa acagagttac catatgcaac atcagatact
     5881 ttgagtttga ctagatcaca gcctcgaagc ttactgtcca aggccatgtt gaacagagct
     5941 aaatcgcgcg ttttaccttc cagttcaagt cggattctga taccccagat atgagatatc
     6001 tgaagtggtc ttttttggcc tatgatacgg tctttgttcc acggtgacgt gttcatactc
     6061 aaatctcctg caatgtggga gatttgagta tggttgtcct tatgagcgag gcgcggaagc
     6121 tcactttagg gcaatctaat tcacatgtca gaggatttat aaaaagagaa tagtgcagat
     6181 tttagggata attacacttg ctatggcata ctgtttggga ccccatcagt ttagggatct
     6241 gaatcgccac agctttggta ggcacctgct ttatggtcac gtgcacccga agatcctgcg
     6301 ggtacgcatg ggtcacgaaa agtacgagtc tacagaagtt aatttacggt tttttgcgct
     6361 cgatgtggct gccagctacc aagtccagtt tacactggat acaactaacg cgctgtagca
     6421 actgcgtggt ggaagctgat taaaggagat aatgatgttc gatgttgacg atactgcacc
     6481 cgatactccc gcccctcgtg aactcagtca ggatgtgcag gcgctgatca acaatggtct
     6541 ggattttctg gataaagccc gtgaagagtt ggaggcctcg aagccgaagt tctccgtcgt
     6601 cagtttctgg actgcggtcg agattttact gaaagttccg ctggcgcacg aacactggtc
     6661 gttggtctgc agcccgaaaa agccaataaa aaagcaggct tacctagccg gtgattttca
     6721 gtcggtgacc tacgaagaaa cccgtgaaag gcttaaggac gtacttgaaa gacccctaga
     6781 cagagaaacg gacagtgctt ttgataaggt cagaaagcac cgcaaccgcg tagttcattt
     6841 ctaccacccc actttcaccg aggctgaaca acgtcaaata ctcaaagaac aagctgacgc
     6901 ctagtttgcc ctgaaccggt tattaaggga tgaatggaaa gttatcttcg gtgtcaaaca
     6961 taattggaag ctggctttcg gtgaaactcg tctgattagg ggaaacaaat tttatgccga
     7021 ggtgcggttt aaacaggtta agcctgaact cgagcagctc tctgaaaaga atattcaaat
     7081 cggtaactgc aatgagtgcc atcagcatgc gacggtaaca gggactgaaa cgaccagtaa
     7141 tgaaaaccgt aagcttgaag ttacgcggtg taaggtttgc accagtgctg taaggaaaat
     7201 taccctagtt tgtccagact gcgaaatacc tcaattgcta cctgagggag atagtgattt
     7261 tgaatgcgaa cactgcgact acacttcgga taggtataaa ctgctggatg aggagttgtt
     7321 ccacagtgtg gatgaacagc tactctccgt atttcctgct ggatgtacca attgcatgac
     7381 tcctgaaagc gtatgtaaat ttggagacgg ctatctttgc actcagtgtt tcatctatta
     7441 caccgagctt catgtatgtg gttgttgcgg tcacctgagc gatagcgttc ctgaactgag
     7501 ccatatccga ggatgtgaat tttgtgatgg tgaccaacgt tattttgatg actgaattac
     7561 catacataat ctaggctctt cagggatggg ttggacagtc cgctcctggc acagagcgga
     7621 cgatatcact gagctgaagg tccgctgaga gcgaggagcg gaagtttata gacatttctt
     7681 gcattgcgtt gtctaatcaa ttaggagctg gtaacgtgcc ctgagtaaat aagcccgcat
     7741 atgcgggcgc tatgtacgct ctgttttgta tggtgcaaag tgccccgtct atctgaaacg
     7801 ctcaaacagt gcaaccattc cttcaggagc gatatctctg acctgggctc cttgccgctc
     7861 aacccactta ccagctatat gtgaaggggt atcctgacct aaaatttcga agacgaaaac
     7921 cgtcttatcg aagtatcgaa cgcatttaag atgagtgttt atgtaggact taagcggttc
     7981 tgaaagtcct gagcgtccta cccgatcaga gatgtcttgg aacatctggt caaggttctt
     8041 tccagcgtgg ttcgcttcgt gatcaacacc agttatatgg aagccgttat aagataaagc
     8101 cttgcttccg aaaatctcct gaactcttgt tgcagtatgc gcattttcag caacccctac
     8161 aagaacatag cccttgtgtc ctctgccgat attagcaata gcagcgcagg tctccataat
     8221 tttttcgaag gagcgttcat caaaggaagg tgatgggtct aaagtataga aaccttgttt
     8281 aaaatcgtag gccgcctgct cagttattga gttagtgagt aaattttgga gcttcgtgat
     8341 ccaatgaact tttgcaggat ctggattttg atcgttttca aaaaagccct gaatccaacc
     8401 aattattgaa tcgaccattc tactccggtt gtcagctccc catctccctc catcttgaac
     8461 ctgaatattc ctaccgcaat ctttcagccg cccaatgagg cccgcactat ctgaaaccac
     8521 catacccctt ttaattatca ggtcatgcat ggcgagaaag accacctgaa agtaacgggg
     8581 gactggatta ccaccatttc cgttaggaaa aatcaaacca gagaatgtcg tttgagcctg
     8641 actaagaagt aggattatcg catcatgtac tctttgataa tcaagatcga ctagctcccc
     8701 ggttctcttt ttaatagctt gatcaatcgc ttcgaatctt gctttgctgg ctaaagcttc
     8761 cggggatatt gctccatagt agtcatctag aagctctgta cgcgaggcca gaggtttgtc
     8821 agaaaccata taagcaagaa tgtccgccac tatctcttca tcccggctct gtcttaactg
     8881 ctccttcgtc aatattccgt taacaatcca gaagacgcta tcggcactaa tgccgtagtc
     8941 taaatccttg ttcgtgatgc taattttttt catctcgctt aagagcaaga gatccgcgtt
     9001 agatgtgtca ccacgaactc ttgcggagat cttccgaaca cattccgcaa acacatttgt
     9061 agcacctgct gcacgcaact cctgtcgtga gagctgacgt ccacctgagt taattcgcct
     9121 gaacacttcc tcaactgagt ctctctcggc tgcttcataa attgaaagcg ggacaggata
     9181 tgatgctatt gcaagacact ggcttcgatc aaggagtggc atcttctgat caatgactcc
     9241 gcgatctaat aggtctttcg tcgtcgcgaa cgtatttaaa tcaaagtaga atccattaaa
     9301 tgaaaaatcg ttcgagataa aagacatgat ggcattcata cgctgcatcc catcaataat
     9361 ctcgagagtg ccgtcttttc tcccaagagg ttctgctaac aaaatgatag gtatcgggaa
     9421 tcccttgatt atcgagtcaa taaaattttg tttttcctca atagtccaaa tcaatttacg
     9481 ttgatactgt cgattcacaa cgtatttttt atcctgatag ttgataaaca ctcgctcaac
     9541 ttgttcgcca cgaactatta gttcttgttg taacgttgcc attatgttgc tgtcctgagc
     9601 catttataga gatataacga ttaggcaaaa cctgccctac atcacacgta
//
LOCUS       DAZFFG010000153         9400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFFG010000153 DAZFFG010000000
VERSION     DAZFFG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.154"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..984
                     /locus_tag="ACHJ7J_004374"
     CDS             <1..984
                     /locus_tag="ACHJ7J_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFO5002519.1"
                     /translation="FRSWLARVTQMRLKLQQVTSAPDPQAMSQMLARAVFQGKAIDLT
                     DTRDYGSLVAASLGQEWSGFGQSLFVQPLDLAWRQVLVPAAGSLNARWQTTIVDQWNK
                     AFAGRYPFKATGSDASLPLLAQFLRSDSGRITTFLKTHLGGLLHQEGNRWGVAPSASQ
                     GMVVSPDFLRAINQLAELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNLDYFN
                     QMESWQSFTWPGNTYYPGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPLDASR
                     TQLVWITADGNPLKFVMRSELGDGPLALLKLQGFTLPTAIFNVAAGTEGVE"
     gene            987..1256
                     /locus_tag="ACHJ7J_004375"
     CDS             987..1256
                     /locus_tag="ACHJ7J_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002520.1"
                     /translation="MNRSIHPAPVRGLMGSFFTLSENGSFTPVGVFAPMSEKTTGKRP
                     EGQPHSAGERMLLKWRPSQIFNSKDDASPEAVVRFMKETDKQEKE"
     gene            1253..2866
                     /gene="tssA"
                     /locus_tag="ACHJ7J_004376"
     CDS             1253..2866
                     /gene="tssA"
                     /locus_tag="ACHJ7J_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO5002521.1"
                     /translation="MSSPEALLTACAASQEERLSLISRANEALSLWDNWLHPFTSGSE
                     TGDDPAYDDNFQLMREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRD
                     GERGLSEGLLLLVAMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGVDSNEA
                     GLTAGAISLLGSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPREPGRE
                     SAPPDSPQLAAVTSGRDLLDQARLLSRWLNGQPQGWLASHRLMKTVRWDTVDQLPSLD
                     SSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLWQGLS
                     HAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEEDVMY
                     GDEAAIPAVVNGQTDDVLILESEAMEKGDTEGPEAALAWLQSRPGMDTPRHRWLIRLL
                     MARVAEQYGRNDMALHLLGELITRAPQLTLEDWEPALLFEVRARRLRLLRMKAGRSES
                     DKARLMPEMDTLLAGLIAIDPARAMVLCG"
     gene            2900..4669
                     /gene="tssF"
                     /locus_tag="ACHJ7J_004377"
     CDS             2900..4669
                     /gene="tssF"
                     /locus_tag="ACHJ7J_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5002522.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     SVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYMRTIPSLAIVEFSPDWRSL
                     RQAEILAEGFSVLSRPVGPHKTACQYRTTRDVLIQPLHLADVRLHTESDGRSAIRLRF
                     ECPEKVDWSKAGIDKVAIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGW
                     CRPMGFDDNDCLWKKADTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEI
                     DIVLSEAWSSGLPFETENFRLHCAPVINLFTLEADPLILNPLDNEYLLRPLRLQDGHT
                     EIYSVDNIHGSVKNGRHSYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLV
                     LGGRSFELEQLSDKPESLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVLNLTAP
                     TMPLYPPANDRFHWRVMSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVA
                     VKHTLIRRFEKGFMLRGVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQL
                     TLVLQPTGKRLRWKENHSRHVPG"
     gene            4633..5721
                     /gene="tssG"
                     /locus_tag="ACHJ7J_004378"
     CDS             4633..5721
                     /gene="tssG"
                     /locus_tag="ACHJ7J_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5002523.1"
                     /translation="MERESQPARTGLTQALAFNNDIWRASFYRFCQLLELENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEVDEDHPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDIAQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIDNRSGLGAGHRIRLSQRAPLGKTAGEACSRLLMTLETADPNEAEGWLPGGGLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPESRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            5755..6282
                     /gene="tssJ"
                     /locus_tag="ACHJ7J_004379"
     CDS             5755..6282
                     /gene="tssJ"
                     /locus_tag="ACHJ7J_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5002524.1"
                     /translation="MATVIRWWALMLIIACSLLAGCGLTQTVTDGTISITKSIFYRKI
                     KTLHLDFTPRTAINADGAQTPLATMVRVYQLRDRKAVDAADYQMLLRRADTVLKDDVL
                     ASKELLVMPNGSATLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRI
                     LELGDGWLSLVPVKE"
     gene            6287..8485
                     /locus_tag="ACHJ7J_004380"
                     /pseudo
     CDS             6287..8485
                     /locus_tag="ACHJ7J_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
     gene            8804..>9400
                     /locus_tag="ACHJ7J_004381"
     CDS             8804..>9400
                     /locus_tag="ACHJ7J_004381"
                     /inference="COORDINATES: protein motif:HMM:NF017589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxic necrotizing factor Rho-activating
                     domain-containing protein"
                     /protein_id="HFO5002525.1"
                     /translation="MKDDSAMLAKGISSVLCGRGLLISAGHDNCSIKFGVELFRYDLH
                     YQHRDFFPMINEGFYSDNANCFSHVVNISGGKSITSKPGGAGVIGHYWSSHVLDDDIK
                     VFHVRNGQSGTVCAKIHLADVSDTVIFSSGALSGCTMIYAIKDGYFYAFHTGQKPGDN
                     NWRTGGDGVQSLASLVSIINGKKITECNRNEDLLQVFAD"
BASE COUNT         2107 a         2442 c         2737 g         2114 t
ORIGIN      
        1 ttccggtcct ggctcgcccg tgtaacccag atgcggctga aacttcagca ggttaccagc
       61 gccccggatc cgcaggcgat gtcgcagatg ctggcccggg ccgtgtttca gggcaaagcc
      121 atcgatctga cggacacccg cgattacggc agcttggttg cagcaagtct ggggcaggaa
      181 tggagtggat ttggtcagtc gttgtttgtg caaccgctgg atctggcctg gcggcaggtg
      241 cttgtcccgg ctgcgggcag cctcaatgcc cgctggcaga caaccatagt cgatcagtgg
      301 aataaggctt ttgcggggcg gtacccgttt aaagccaccg gcagcgatgc ctccctgccg
      361 ttactggcac agttcctgcg tagcgactcg ggtcggatca cgacgttcct gaaaactcat
      421 cttggcgggc tcctccacca ggaggggaat cgctggggtg ttgctccttc cgcaagccag
      481 gggatggttg tcagtccgga tttcctccgg gcgattaatc aactggcaga gctgtcagat
      541 atcgtgttct ctcagggcga tgcgggggtt cacttcgaac tgatggcgcg tccttcacgg
      601 gatgttgcca gagtgcagct taccctcgat gaacagaatc tggattactt caatcagatg
      661 gaaagctggc agagcttcac ctggccgggg aatacttact atccaggtgt gtcgctcagc
      721 tggcggagtg taagcagtgg tatgcaactc tacgccagtc accaggggaa ctgggggttt
      781 attcgcctgc tggacgaagc cctgatcacg ccacttgatg ccagccgcac acaactggtg
      841 tggatcactg ccgatggtaa tcctctgaaa ttcgtcatgc gcagcgagct gggcgacggc
      901 ccgcttgcct tactgaagct acaggggttt acgctgccca ccgccatttt taacgtcgcc
      961 gctggtacgg aaggtgttga gtaaagatga acaggagcat tcatccggcg ccggtccggg
     1021 gactgatggg ctcatttttc accctttcag agaacggttc cttcacgccc gttggcgttt
     1081 tcgcccccat gtcagaaaaa acaaccggta agcgaccgga agggcaaccc cactcagcag
     1141 gagaacgtat gctgctgaaa tggaggccat cacaaatctt caacagtaag gacgacgctt
     1201 ctccggaagc ggtcgtgagg tttatgaaag agactgacaa acaggaaaaa gaatgagttc
     1261 accggaagcc ctgttaacgg cctgcgccgc cagccaggaa gaaaggctga gcctcatttc
     1321 ccgcgcaaat gaggccctgt cgctctggga taactggctg catccattta cctccggatc
     1381 tgaaaccggg gacgatcctg catacgatga taattttcag ctgatgcgtg aagagatcaa
     1441 taagatttca ggtaccgatt cagcgctgtt gtgcgaactg gctcagaagt gcctttgcga
     1501 atgcgccaga gatattcgtg tcgtgacctg gtacgtgctg gcccgtctca cccgggatgg
     1561 tgaaagaggg ttgtccgaag gcttattact tcttgttgcg atgctgaccc gctacggaca
     1621 ggcatgtcat ccccggcgtc ctgccgcccg gaaagctgcg ctggagtggc tgaacagtgc
     1681 aaaagtgact gatgcactgc tgttatggcc cggggtggac agcaatgagg ccggtctgac
     1741 ggcgggtgcc ataagcctgc tgggatccgc cgtggcagac tggccggaag cggagaagcc
     1801 gtcttttgcg gggctttgtt ctgcgcttga aaatcgcctg gcacgttcag ggggcatgga
     1861 ggcactggtc ccccagaaca gcagcccccg ggagcccggt cgtgaatcag ctcccccgga
     1921 ctcgccacaa ctggcagcgg tgacctcggg ccgcgactta cttgaccagg ccagattact
     1981 ttcccgctgg ctgaacggac agccgcaggg ctggctggct tctcaccggc tgatgaagac
     2041 ggtgcggtgg gatacggtcg atcagctccc ttcgctggac agtagcgggc gtacccgcct
     2101 ggtccccccg aaaaccggat atcgcgctca gcttaagcga ctctggcttc agaaaaactg
     2161 ggttgagctg attgaactgg ccagtcagat gtattgcgaa ggggtgaatc atttctggct
     2221 cgatcttcag tggtatctct ggcagggact cagtcatgct ggtcagccct gggattcctg
     2281 gtctgacgct gtcctgtcag atctgcggct gttgcttaaa cgtctcccgg gccttgaagg
     2341 gctggcatgg aatgatggta ctccctttgc tgacgaggtc accctcgcct ggatcgctga
     2401 aaaagtgaat gaagaagatg tgatgtacgg cgatgaagct gccattcctg ccgttgttaa
     2461 tggccagact gacgatgtgc tgatccttga gtctgaagcc atggaaaagg gagacacgga
     2521 agggcctgaa gcggcgcttg cctggctcca gtcccggccg gggatggata caccacgtca
     2581 tcgctggctg atacgcctgc tcatggcccg ggtggcagag cagtatggcc ggaacgatat
     2641 ggcgcttcat ctgcttggtg aactcattac ccgtgcgcca cagctgacgc tggaagattg
     2701 ggagcctgct ttgttgtttg aagtccgggc ccgacgcctg aggctgctac ggatgaaagc
     2761 cggtcgcagt gaatctgata aagcccgtct gatgcctgag atggatacgc tgctggcggg
     2821 gctgatcgcc attgacccgg cccgtgccat ggtgctctgt ggctgatgtt ctctttaatc
     2881 attttcagga atgccgtgca tggatgattt aaccctgcgc tattacgaag cagagatgcg
     2941 ctatctgcgg gaagccggta aagagtttgc ccgtgcccat cctgatcggg cagcaatgct
     3001 gaatctggat aagcctggcg cctgcgatcc cagtgtggaa cgtctgtttg aaggctttgc
     3061 cttcctgatg ggacgtttgc gtgaaaagct ggatgatgac ctgccggagc tcaccgaagg
     3121 tctggtgagt ctgctgtggc cgcactatat gcggactatc ccgtcgctgg ccatcgttga
     3181 gttctcgcct gactggcgta gtctgcgtca ggccgaaata ctggctgaag gcttttccgt
     3241 tctgtcccgt cctgtcgggc cgcataaaac cgcctgtcag taccggacga cccgtgatgt
     3301 gttaatacaa ccgctgcacc ttgcagatgt ccgcctccat actgaatccg atggccgttc
     3361 tgctatccgt ctgcgcttcg aatgccctga aaaagtggac tggagtaaag ccgggatcga
     3421 caaagtcgcc atttttctta acgcagaggc gccggtaagc gctgcactgc atctggcaat
     3481 gacccggcgt gtacatgcca tgtatgcgcg tcatgccggg acatataccg gacgtcatca
     3541 gtttgacggt tggtgcagac ccatgggctt tgacgacaat gactgcctgt ggaagaaagc
     3601 cgatactgcc ttcagtggtt accagttgct gctggagtat ttcagcttcc gtccgaagtt
     3661 tatgtttgtt gaactgcgcg gcctcgacac catcgggctc actgcggcta gtacctggtt
     3721 tgaaatcgac attgtgctca gtgaagcctg gtcatccggt ttgccttttg agacagaaaa
     3781 cttccgcctg cactgtgcgc cggttatcaa tctctttacc ctggaagccg acccgctgat
     3841 ccttaacccg ctggataacg aatatctgct caggccgctg cgtcttcagg atggtcacac
     3901 cgaaatttac agcgtcgata atattcacgg ctcggtgaag aatggcagac actcttatgt
     3961 cccgttcacc agcttccggc accggggcgg tatgatgcgc cacgatgctc ctgaacgtta
     4021 ttaccacacc cgcgtgaaac ggggaccctc cggcctgtat gacacatggt tagttctcgg
     4081 gggacgctcg ttcgaactgg agcagctgtc tgataaaccg gagtccctct cgatgcgaat
     4141 caccgcgacc aacgggcagc ttccgcgcag ggcactggaa agtacgctcc ttgacagggt
     4201 ggtcaaaacc ggcaaagtcc ccgtcagagt cctgaacctc accgcaccga cgatgccact
     4261 gtatcccccg gcaaatgacc ggtttcactg gcgggtgatg agtcacctcg gatcaaactt
     4321 tctcagcatg atggacaatc cggaggtgtt gcggggaacg ctggcgctct atgactggac
     4381 cgacgatgaa atgaaccgcc gccggcttga agccatcgtt gccgtgaagc acaccctgat
     4441 ccgccgtttt gaaaagggct ttatgctccg tggcgtggat attgaggtta cgctgaatat
     4501 ggataatttt gccggcgagg gtgatgtgaa tctcttcggg gagatgctgc accgtttctt
     4561 tgcgctctat gctgatgttc accttttcaa ccagctcaca ctggtactgc aacccacagg
     4621 gaaacgactg agatggaaag agaatcacag ccggcacgta ccgggctgac gcaggccctg
     4681 gcctttaaca acgatatctg gcgggccagt ttttatcgtt tctgccagct gctggagctg
     4741 gaaaatccgg acgggccaaa actgggcacg accagccatc caggcgatga tccggtgcga
     4801 ttcagaccct ggcccgggat gggattcccg gtcagcaccc tgaaagcggt ggaggtcgac
     4861 gaagaccatc cgacattacc cccaacaatc cgcacgacat ttctcggaat gtatggggtg
     4921 gactcacctc tgccgacctc ctggctggat gatattgcgc agcgccgtga aggacatgag
     4981 gcgctcacct catttctgga tattttcagc caccgcataa ccacgcagta ctaccggatc
     5041 tggcgaaaat atgcgtatcc ggccactttc gaggagggag gacgcgatgc cacatcgcag
     5101 tgtctgctgg gactggtcgg actcggtata cccggcaccg ctgaacaggt ggcaacgccg
     5161 gtttcccgtt tcctggcctt actgggtgct atgcgattac ccacccgcaa tgctgaaggc
     5221 atccgggctc tggtgagtct gctggcaccg gatacccgcg cacttatcac cgaacccgat
     5281 ccggttaagg tccatatcga taaccgcagt gggctggggg ccggacaccg catccgtctt
     5341 tcgcagcggg cgccgctggg taaaacggca ggggaagcat gcagccggct cctgatgaca
     5401 ctggaaacag cagatccgaa cgaggcggaa ggctggttgc ccggtggggg cctccatacc
     5461 gatttgctgg tgctgctgcg cgtttatctc ggctaccgca gtgacgtccg gctgcgcctc
     5521 actgtccccg tgcggttgtt gcctgagtcc cgtctgggca aaggcaggcc tgttcagctg
     5581 ggccgtaccg gtttgctggg gctgaaagcg ggcaaactca gcaatggtcg ggagagtctt
     5641 acggtcagtc tgggctgcta cgaagggctg aaatgtgctg cactaccgcc tgctgaagac
     5701 gggcattacc gcttcgaata atcacctgtt ctctctcaat aaaggaacgc ctgaatggca
     5761 acagtaatcc gctggtgggc gctgatgctt attattgcct gtagtctgct cgcaggctgc
     5821 ggtctgacgc agaccgtcac ggacggcacg atcagcatca cgaaatccat cttctacagg
     5881 aaaattaaaa cgctgcatct tgattttact ccgcgtacag ccattaacgc tgatggtgca
     5941 cagacgccgc tggcaaccat ggtgcgggtt taccagctca gggatcgtaa agcagttgat
     6001 gccgccgatt accagatgct gctgcgcagg gctgataccg tacttaagga cgacgtgctg
     6061 gcctcaaaag aactgctggt gatgcccaac ggcagtgcga cgctcaatat gcccatggat
     6121 gaagacgcgc agttcgtggc tgtggtggga ttgtttaacc gaccggatca gaaggacaat
     6181 cgctggcgcc tggtactgac ccgtgatgac ctcaacccgg ataagccccg catacttgaa
     6241 ctgggtgatg gctggctgag ccttgtgccg gtaaaggagt gacgtgatgg ataaccggtc
     6301 agcgcttttt gacggacaga cccgttactt tctggaaatt aatggcagtg cagtaaagcc
     6361 tgatgttttg cactttcgcg gcagagaagc actgagtgag ccgttcagat gggacatcgg
     6421 gttcacctcc cgggatgcga acatttctcc ggaagaggtg ctgatgaaat atgcgtcgtt
     6481 acggatgcgt aacggcaaaa atgtccatgg cattgtgacc cgtctggaat ggctgtccac
     6541 atcaaaagac cagtcccact accggcttac actcagctcc cgtctggcac tgctggagta
     6601 cacccggcag tgcgccgtat ttctgaacca gtccgtccct gaggtggtgg agcaggtgct
     6661 gcgtaaacac gggctggagg gagctgattt tgaattcagg ctggaacgcg cttatccgtc
     6721 gcgtgaaatt atcacccagt ggcaggaaac ggatttgcag ttcattcagc gcattctgtc
     6781 tgaggtgggg atttactggc gcacggagat ggatgatgtg cgtggtatgg atacgtgcat
     6841 ttttgccgac agccagctta actaccagtt tgatgtgcgc ctgccctgtt gtgagccgtc
     6901 ggggcttttt gacggtgctg cagaatctgt ctgggatgtg cggacctggc acaacgtagc
     6961 taccggcact gtcaccacgc gtgattacaa ctaccggact gcctccatgt cgatggatgc
     7021 gacggtcagc gtgcgtaatg atgcagtcac caccggggag cattaccgct atgtatcggc
     7081 ttatcgtgaa gcgggggatg ataccagcac ggagccggaa accgaatctg gcgcgtttta
     7141 cgcccggctt caccacgagc gggagcttaa caggtcggcc cgcattcacc tgttcagcaa
     7201 cgccgcacac ctctcccccg ggcaggtgct ggagccgcag ggggatgtca ttacggacct
     7261 gaaggaaggg attgtcctca cactggtgac ttaccgggga gcccgtgatt ctcggctgca
     7321 tgtgtcagcc tggggaatgc cttacaccga acgttactgt ttccgtccgg cggaaatccc
     7381 gcgcccgctt atcccgggca cgcttccggc acgtattgag agtcgcgaga agaacgatat
     7441 ctacgcccat ctggacggtc aggggcgtta ccgggtgaag atggactttg atcgcaacag
     7501 tacagaacgg ggttacgctt atctgtggtt gcggctggcc aggccctatg ccggggatac
     7561 ctgtggtttc cactcactgc ttatcgacgg aactgaagtg gccattgtat ttgatggcgg
     7621 agatccggat cgtccctaca tcgcttatgc cctgcatgat tcagagcatc cggacattgt
     7681 caccagtgac aatcatacgc ggaacgtgtg gcgcacaccg gcgaacaata agctgcggat
     7741 ggaagacagg cgcaacgagg agcacatcaa acttgccacg gaatatggca tgtcgcagct
     7801 taacagcggg cacatggtgg acagcaaggg gcaacttcgc ggtaaaggca cagagctgcg
     7861 cactgatgag tggggagtcc tgcgagcagg taggggacta tttgtcagtg cggatgccca
     7921 ggcgaaagcg cagggtgatg ctctggacat ggatgccgcg ttgaaggaaa tcgacaagct
     7981 taatctacaa ttgcagcaac tggaaatcgc cgcagaacag gctcaggcgc tgaaggcgga
     8041 tattgacagc cagatacaga tgtttgagca gcggctgaaa ccgctcaatg aagctgtggt
     8101 tttctcggcg ccggaaggca ttgccctgac cagtgatgaa tatatgcaga tgagtgcagc
     8161 acgaaatgtg gctgtcaacg ccggaggtga tatcagcacc ggaacaatgg gcaacctgag
     8221 cgcgctggcc ggagagaggc tggggctttt tgcccggacc ggacaactga gcgtcaaatc
     8281 cggggaggga ccggtagagg tacaggcgca aaacggcagt atgcggttgt ttgcagagaa
     8341 ggcgctgatg ctgagctcgg caagtgatat ctcctttgcc gggaaaaaac gtatcacgct
     8401 gattggcggg gggagctatc tgcgacttga ggccgggagg gtcgagtatg gtacgaatgg
     8461 ggtatattta tgtaaagtta aaagaataaa tctcacctca gccgaggaaa tatcatccga
     8521 agcaacatgg gctaatcaaa atactgtgaa aaccttaaca gataaagata agtctgacgc
     8581 tatactaaag acatattccc ggacgactaa tgtagaaaag ggaaaaagac cctcattaat
     8641 taaaagatta agcttcagaa aaaataccag gtctgaacca acgtcaccaa cgtcaccaac
     8701 gtcaccaacg tcaccaacgt caccaacgtc accaacgtca ccaacgatat ctgtacccgt
     8761 ttacggaaga aagatttcta taatgaagag actgagtctt aacatgaaag atgattcagc
     8821 aatgttagca aaaggaatat cctcagtttt atgtggtcgt ggtcttttaa tttccgcagg
     8881 acatgataat tgttcaatta aattcggtgt tgagttattc cgctatgatc ttcattatca
     8941 gcatagggat ttttttccaa tgattaatga aggcttttat tctgataatg caaattgctt
     9001 ttcgcatgtt gttaatatat caggtggaaa atcaataaca tcaaaacccg ggggggcagg
     9061 cgtcatcggt cattattggt catcacatgt attagatgat gacataaagg tttttcatgt
     9121 aagaaatggt cagtcaggaa cagtctgtgc caaaatccat ttggcggatg tgtcggacac
     9181 tgtaattttt tcatctggtg cactgagcgg gtgcacgatg atttatgcaa taaaagatgg
     9241 gtatttttac gcttttcata caggccaaaa acctggggat aataactgga gaactggagg
     9301 agatggcgtt caatcccttg catctcttgt atcaataata aacggaaaaa aaataaccga
     9361 atgcaataga aatgaggact tgctgcaggt ctttgctgac
//
LOCUS       DAZFFG010000154         9301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFFG010000154 DAZFFG010000000
VERSION     DAZFFG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.155"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            116..478
                     /locus_tag="ACHJ7J_004382"
     CDS             116..478
                     /locus_tag="ACHJ7J_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002526.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKGMGKTTEE"
     gene            complement(759..1217)
                     /gene="ybbJ"
                     /locus_tag="ACHJ7J_004383"
     CDS             complement(759..1217)
                     /gene="ybbJ"
                     /locus_tag="ACHJ7J_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5002527.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1214..2131)
                     /gene="qmcA"
                     /locus_tag="ACHJ7J_004384"
     CDS             complement(1214..2131)
                     /gene="qmcA"
                     /locus_tag="ACHJ7J_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5002528.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2277..2954
                     /gene="fetA"
                     /locus_tag="ACHJ7J_004385"
     CDS             2277..2954
                     /gene="fetA"
                     /locus_tag="ACHJ7J_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO5002529.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHSVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2941..3720
                     /gene="fetB"
                     /locus_tag="ACHJ7J_004386"
     CDS             2941..3720
                     /gene="fetB"
                     /locus_tag="ACHJ7J_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO5002530.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3783..4637)
                     /gene="cnoX"
                     /locus_tag="ACHJ7J_004387"
     CDS             complement(3783..4637)
                     /gene="cnoX"
                     /locus_tag="ACHJ7J_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO5002531.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4698..5507)
                     /gene="ybbO"
                     /locus_tag="ACHJ7J_004388"
     CDS             complement(4698..5507)
                     /gene="ybbO"
                     /locus_tag="ACHJ7J_004388"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5002532.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5497..6120)
                     /gene="tesA"
                     /locus_tag="ACHJ7J_004389"
     CDS             complement(5497..6120)
                     /gene="tesA"
                     /locus_tag="ACHJ7J_004389"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO5002533.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6091..6777
                     /gene="ybbA"
                     /locus_tag="ACHJ7J_004390"
     CDS             6091..6777
                     /gene="ybbA"
                     /locus_tag="ACHJ7J_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO5002534.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6774..9188
                     /gene="ybbP"
                     /locus_tag="ACHJ7J_004391"
     CDS             6774..9188
                     /gene="ybbP"
                     /locus_tag="ACHJ7J_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO5002535.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCCGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2103 a         2401 c         2569 g         2228 t
ORIGIN      
        1 gcgaaataaa ttctgtttct ggactaaacg tgaagcggtt cctgatgtgt gctaaaatgc
       61 gtttctttga taaatctcaa tttttaagag gtttacgcca ggcaggaaaa tttaaatgaa
      121 tgaatttgag aagattttta atgaaatgaa tctggaccgg gcattgctac cgatcctttt
      181 tcgttcgaac cggtcgacag tgtggaagta tttaagcggc gatagtacgg ctcctgcctc
      241 tgccatgtcg ctgattatgc tgttgcagtt gatacagaaa cgaaaccccg atctgttagc
      301 cgaatggtta acgctttctg atttcaccat tccgccagaa gtttacctgg accagcctga
      361 ttactggaaa gggtgggtgt atacccaaca caaggtcaat aaaaacgtcc tggaatatct
      421 aaaaaaacac tatccggatg aagaccaaaa aggtatgggt aaaaccacag aagagtaaat
      481 tgtcattacc cgcccacatg ccggatgcgc acagactggc agtgttagta ggcatgataa
      541 tacgcgtcag cgtcgcatca ggcatcggcg cataaccgcc ggatgcggcg taaacgcctt
      601 atccggccta cgattcgggc gcagacttcc aggcatgata atacgcgccc gcctcgcatc
      661 aggcttcggt gcacgactgc cggatgcggc gcaagcgcct tatctggcct acgattcggg
      721 catattttgt aggcatgata agacgcgtca gcgtcgcatc aggcgatgac cgcacggatg
      781 atcagcgtta tcccttctat cgcaatgact tcaacatgcg tacctgcgcc gagatcctcg
      841 ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc
      901 ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgactg
      961 tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg
     1021 agcagcgtca ggacggcaaa catcacccct tgccactccc aacccagcgg caccagccag
     1081 accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc
     1141 agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg
     1201 accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agctcggcaa
     1261 tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcacc actttgctgt
     1321 tactggacga accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca
     1381 ccgcctgaat atcgccggag gcaatggctt cagacaccat tttggtggcg cgggcttctg
     1441 cttcagcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg
     1501 ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc
     1561 cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg
     1621 aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc
     1681 cccacgggtt ggtggcctca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct
     1741 gagagagcat ttcgtcaagt tccattgaac ccagcacggt acggatgtta gtcatggtca
     1801 ggttgatgat cgccagctcc agattgctga cttcataagc cgcgcgtggc gcgtcaatca
     1861 cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct
     1921 gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa
     1981 acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta
     2041 ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga
     2101 caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata
     2161 atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc
     2221 cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc
     2281 aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt gatacgaaga
     2341 ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt
     2401 ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg
     2461 gaacgttact gtttgaaggg gaggatgtca gcacactaaa gccagaaatc taccgccagc
     2521 aagtttctta ctgcgcccag acgccgacgc tgtttggcga tacggtatac gataacctga
     2581 tttttccctg gcagatccgt aaccagcagc ctgacccggc catttttctc gattttctcg
     2641 aacgcttcgc tttgccggac agcattttga cgaaaaatat cgccgagcta tctggtggtg
     2701 aaaaacaacg catctcactg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg
     2761 atgaaataac cagtgcgctg gatgaaagta ataaacatag cgtcaatgag atgatccatc
     2821 gttatgtgcg cgagcaaaat attgccgtgc tatgggtgac acacgataaa gacgaaatta
     2881 atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct
     2941 atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct ggtggtggtg
     3001 gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct ctggagcgtc
     3061 gggcgcgcga taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg
     3121 gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac
     3181 gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg
     3241 gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg
     3301 atgcaggtga tccctatcgc cgggatgata gccggtaacg ccatggtagc ggtggggttg
     3361 tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg
     3421 agtcttggcg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg
     3481 gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg
     3541 atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg
     3601 gtgactttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc
     3661 tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga
     3721 tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg atcaacggaa
     3781 tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta
     3841 cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga
     3901 tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctggtgc
     3961 aattgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt
     4021 tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc
     4081 agcccctggt agcgggtgtc ctgatcctgt aacggaatgg ttttcagcac cgcttccgca
     4141 tcttcagaac ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc
     4201 tgattcgaca actgccaggc gtcttttagc aatggcaggg catcggtgta attgccttcc
     4261 tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc
     4321 agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccgtctac tggttgcccg
     4381 ttctggaaca gatatacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc
     4441 tccgcgtcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcgaggctt
     4501 tccagaattg gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc
     4561 accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca
     4621 atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct
     4681 taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc cggcagcagg
     4741 cgcttaagca ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc
     4801 tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa cgtaaagcgg
     4861 gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg
     4921 aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgtagc
     4981 tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca
     5041 cgacccggcg tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg
     5101 tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg
     5161 gaaaactgct gttccatctg cgcacggctg atggtggaaa gggggccata catgccgaat
     5221 ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac ctcgtcggct
     5281 gcgcgatcaa cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgctg
     5341 ttcatgcgct caacatcatc cggtttccgg caacctgcca gcacatgaaa accctggcgt
     5401 tttaattcga gagcgctttc caggccaatt ccactggaac atccggtaat taagaccgat
     5461 ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg
     5521 ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat
     5581 accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag
     5641 cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg
     5701 gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt
     5761 gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa
     5821 accgtcattg ccgcccagtt caaccagcac ccagcgcggc tgatgctgtt tcagcagagc
     5881 cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg atgctggcat tgactaccga
     5941 cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg
     6001 atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg cggcacggaa
     6061 ggttaacagg accaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat
     6121 catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag
     6181 ctggttgtca aacgtggcga gaccatcgcg ctggtgggcg agtcgggatc gggtaagtca
     6241 accttgctgg cgatcctcgc cgggctggat gacggcagca gtggcgaagt gagtctggtg
     6301 ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc
     6361 ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag
     6421 cttccggcgc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggagc gaaagcgttg
     6481 ctcgaacagt tagggctggg taaacgtctg gatcatcttc cggcacagct ttccggcggt
     6541 gaacagcaac gagtggcgct ggcccgagcc tttaacggtc gacctgatgt gctgtttgcc
     6601 gacgaaccca ccggcaacct tgaccgccag accggcgata aaattgccga cttgctgttt
     6661 tccctcaacc gtgagcatgg caccacgttg attatggtga cccacgacct gcaactggcg
     6721 gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg
     6781 cacgctggtt ctggcgcgaa tggcgctcgc cgtcgctatt aattgtctgg ctggcgctaa
     6841 gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct
     6901 taagccaaca aagccgggag tttatggcgg gcgatcgggc gttgcgcagt tcgcgcgaag
     6961 tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga
     7021 ctttcgccac catgacattt gcaggcgaca caccgcagct ggcgaacgtt aaagcggtgg
     7081 atgatatcta cccgatgtat ggcgatctgc aaaccaatcc ccctggcctg aaaccgcagg
     7141 cgggcagcgt attgctggcc ccacgcctga tggcactgct taacctgaaa acgggcgaca
     7201 ccattgacgt gggcgatgcc actttgcgga ttgccggaga agtgattcag gaaccggatt
     7261 ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata
     7321 aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ctataaattc ggcggcaacg
     7381 agaaccaact cgacggctat gagaaatggt tgttacctca gcttaaaccc gaacaacgct
     7441 ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcagcagt
     7501 tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg gcggtggcga
     7561 tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag
     7621 ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag
     7681 ccgttaccgg tggggcgata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc
     7741 ccgttctgcc tgctgcacta ccgccagcca gcctctggcc atggctatgg gcgctgggca
     7801 ccatgacggt catctcgctg ctggtggggc tacgaccata ccgcttgttg ctggcaacgc
     7861 agcctttacg cgtattacgt aatgatgtgg ttgcgaacgt ctggccgctg aagttttatc
     7921 tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc
     7981 tgctttgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt gtgctgggct
     8041 ggatgctgct gaatgtactt cgccgcatga cgctgaaatc gctgcctctg cgcctggcgg
     8101 ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct
     8161 cctttatgct gctggcactg ctgctggtgt tgcgtggcga tctgctcgac cgctggcagc
     8221 agcagctacc tccagaaagc ccgaactact ttttaattaa catcgccaca gaacaggtag
     8281 caccgctaaa agcgttcctc gcggaacatc agatagtccc ggaatcgttt tatccggtgg
     8341 tgcgggcgag actgacggcg attaacggca agccgacaga aggcaatgaa gatgaggcgc
     8401 tcaatcgcga actcaatctt acctggcaaa atacgcggcc cgatcataat ccgattgtcg
     8461 ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga agaggggctg gcgaaacgct
     8521 taaacgttgc cctcggtgat accgtgactt ttatgggcga tacccaggag ttccgcgcta
     8581 aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc tattttattt
     8641 tccctgaagg ggcattagac gggcaaccgc agagctggct taccagtttc cgctgggaga
     8701 atggcaacgg catgttgacg caactcaacc gccagttccc gaccattagc ctgttagaca
     8761 ttggcgcgat tttaaaacag gtcggccagg tgctggagca ggtaagtcgg gcgctggagg
     8821 tgatggtagt gctggtcacc gcctgcggta tgttgctgtt gctggcacag gtgcaggtgg
     8881 gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg gggaaaaaac
     8941 tgctgcgcac gacattgtgg tgcgagttcg ccatgcttgg gtttgtttct ggcctggtgg
     9001 ccgcaattgg tgcggaaacg gcgctggcag tgttgcaggc gaaagtgttt gatttcccgt
     9061 gggagccaga ctggcgattg tggattgttc tgccgtgctg cggtgcgctg ctgctgtcgc
     9121 ttttcggcgg ctggctgggc gcgagactgg ttaagggtaa ggcgctgttc aggcagtttg
     9181 cggggtgatg agagtaaata aggggcgttc tggttgaatg gaacgccttg tttaaattaa
     9241 ttctacaata tgtttcattg atgatgttat tgaattggtg atttcctgtc tttctattgc
     9301 t
//
LOCUS       DAZFFG010000155         9290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFFG010000155 DAZFFG010000000
VERSION     DAZFFG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.156"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            130..924
                     /gene="hyxR"
                     /locus_tag="ACHJ7J_004392"
     CDS             130..924
                     /gene="hyxR"
                     /locus_tag="ACHJ7J_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO5002536.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLPGQRKNISKKRPGLL"
     gene            1078..1839
                     /gene="hyxA"
                     /locus_tag="ACHJ7J_004393"
     CDS             1078..1839
                     /gene="hyxA"
                     /locus_tag="ACHJ7J_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO5002537.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVCNITYIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1881..2375
                     /locus_tag="ACHJ7J_004394"
                     /pseudo
     CDS             1881..2375
                     /locus_tag="ACHJ7J_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2522..3190
                     /gene="ykgH"
                     /locus_tag="ACHJ7J_004395"
     CDS             2522..3190
                     /gene="ykgH"
                     /locus_tag="ACHJ7J_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO5002538.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGMLVNCDSNNNFACQTTMTEDIIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQAYIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKHKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(3433..4128)
                     /gene="ykgG"
                     /locus_tag="ACHJ7J_004396"
     CDS             complement(3433..4128)
                     /gene="ykgG"
                     /locus_tag="ACHJ7J_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5002539.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTELNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(4121..5548)
                     /gene="ykgF"
                     /locus_tag="ACHJ7J_004397"
     CDS             complement(4121..5548)
                     /gene="ykgF"
                     /locus_tag="ACHJ7J_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5002540.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(5559..6278)
                     /gene="ykgE"
                     /locus_tag="ACHJ7J_004398"
     CDS             complement(5559..6278)
                     /gene="ykgE"
                     /locus_tag="ACHJ7J_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5002541.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(6806..7660)
                     /gene="rclR"
                     /locus_tag="ACHJ7J_004399"
     CDS             complement(6806..7660)
                     /gene="rclR"
                     /locus_tag="ACHJ7J_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO5002542.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGMDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRANFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            7886..9211
                     /gene="rclA"
                     /locus_tag="ACHJ7J_004400"
     CDS             7886..9211
                     /gene="rclA"
                     /locus_tag="ACHJ7J_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO5002543.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIAMILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         2566 a         2181 c         2074 g         2469 t
ORIGIN      
        1 ttaaaaacaa taaaaatcaa acaaattaga tttatatccc aagaaaatta aatattcagg
       61 caattaaacc aaacctgaca ttttgaatca aaatgtcagg ttttgtagct atggcgtgga
      121 cgagtctgaa tgaagaagcc tctggttatt atcagtgcct gtcagtttac caggctggca
      181 ctggagtctc tcatcccagc ggacaggtat atcgtgcggg tgtacagcaa cgtcacgaca
      241 gaggttgaat tcgttttaaa gacctcctgt ggctatctgt tggctgatat cccttcgctt
      301 ccttcggggg agttagttct tctggcttgt cttgtccgct tgcggagtga tgccgggcaa
      361 tggcaggtct gcctgaccgg tgactccgac tggttcgata atgctcctgc tgcaagtctg
      421 tataacggct tcccacgcat cggcaccatg ctgacagcga gtcgttatca gaatcaaatt
      481 atccgctggt tactacgtcc tttgcccggc gatatgagcg atttctggct cctgactgca
      541 cgggagttga gcgtgcttaa gattctgatg cagggcatga gtttcagtga gattgcccgt
      601 tatgaacaac gcagttcaaa gaccctgcat gcgattgcca cacgggcatt aatgaaactt
      661 ggactgggaa ctctgagcga tttcaggctg ctctatacgg gatgcggtga cagcagagtg
      721 aaacggaata tacggctaca tgcaagatat gtcggacaac attccgctca tcgactcact
      781 cagtatgtgc agaacatggt atccggaatt ctttatccag agaaagttgc cgttggtgaa
      841 tcaataaatt ctgacgccat ttccggaaaa gtacttcccg gacaaagaaa aaatatttct
      901 aaaaaaagac caggattgtt atgacacaac agcgttgtta ctacccccca ctgcggcgcg
      961 tacggactcg ctacagtgta ggggccaaaa tatttctact gctctgggct gcaggattgg
     1021 gaggcacgct gtacagcctg tacagcgtgc tggcgtactg acatctgtga gggaaggatg
     1081 cgtttcgttc ttttctgtcc ctcaggaatt gtacctgcac agttcgcagc tctatcaacc
     1141 ggttctgtct gcaatattac ctacatcaga acagaagagg agttacgcaa taaattgcgg
     1201 cacaggccac agagtgtcgt gatatcggca gggcgacctg ccgaatgcgc tgaaatgtgg
     1261 ttccgatttt atcgggatca ctcattcgtg gtggtgttgt gcgtcgcgcc cttttttctg
     1321 ccaccggatg tgagtatttc tggcgttctc aaaaacctga ggttactgaa accaggaatg
     1381 tcagtagagc gtgtcatcag tattgcaaat gcctctggtg ggttttcagg actgaaacat
     1441 gcagaaaatt tgccagttat ggacagttac tctgtcttta tgaatgaggt gaataaccgc
     1501 acaaagacaa tcgtcatgtc agaacgcttc ccggaaaagc aaaagaaggt gttgtcgttg
     1561 cttcttgccg gacatagttg ggaatacagt gcgcagtttc tcaaaacggg gataagacag
     1621 atatggcttg ccgagcagtc gctaaaaaag cgttggggaa taccggacag catgtcattg
     1681 cgggaagccc tgctgctgag cagcaataaa tttcacggtg atggaaatgc attagaaaaa
     1741 acgaatgcca tgacattgcg tgaaaacgga aataccaaca ggtattccgt ggtggtaaat
     1801 gcagggacgc aggctttatc tctgcataaa tataaataag tgggaaattg aaattagtta
     1861 ccagaatgga gaatttcttc atgaaaaaca gtaaggcatt ttaccgcagc gcattagcga
     1921 cagctattgt tatggctctt tctgcaccag cattcgctgc tgataacgcg gtatcaacta
     1981 cgtcggttac gttggataaa aataacacga ctctgaatca agatgttgtt attagcaata
     2041 cagcagacca acagattaca gccgtcacaa ttaatgcggc agataaagac cttaatgtta
     2101 cttttggcgg tcatgatatt accgccgaat caacggtaga ccaaaacttc gttgacggtg
     2161 taaaagttag cgatgacaaa aatgttgtga ttaaagctac aggctccacc atcacagctc
     2221 aaggtgaagg tacctatgtc cggattgcaa tggtcattga ttcaactggc gatgttgtta
     2281 atggcgataa ttttgttgca aataatgaaa aaagatatgt gacagggata tcattggaag
     2341 gaacaacggg aaagaatgta acgctaaatt atacttccag cagacagcat actactgcct
     2401 gcaggctata aatatccagt attcatgatg ctggtttttt gctgatattt aacccgaaca
     2461 ttcctgatac agactattaa tacaaactta tctgcgacag atttcatttt ctggatatat
     2521 tatgaatgaa caaatcaaac aggatatcga tctgattgag attttatttt atctgaagaa
     2581 aaagattcgt gttatccttt ttattatggc tatctgtatg gcaatggtgc tgttgtttct
     2641 gtatatcaat aaagacaata taaaagtgat ttacagccta aaaataaacc agacaacacc
     2701 cggcatgctt gttaactgtg atagcaataa taactttgcc tgtcagacca caatgactga
     2761 agatattatt cagcgaatta ctacattttt tcacaccagc ccagatgtca agaacagaga
     2821 aataaagctg gaatggtcag gagataagag agatttacca actgctgaag cggaaatatc
     2881 tcgcgtgcag gcgtatatta tcaaatggta tgcgtcagaa taccataatg gcaggcaagt
     2941 tctcgatgag atacaaacac cttcagcaat taacagtgag ctttatacaa aaatgatata
     3001 cctgaccagg aactggtcat tgtatccgaa cggtgatggc tgtgtaacta taagctcacc
     3061 agaaataaaa cataaatacc ctgctgccat ttgcctggct ctgggatttt ttctaagcat
     3121 tgtgatttct gtaatgtttt gccttgtcaa aaaaatggta gatgaatacc aacaaaactc
     3181 tgggcagtaa taaacagcat tctgatagag agtgcctgta taaagctgaa gtgacaatga
     3241 ccgggaaggg aacccgtttt caggaatgga tgcctgtctt tttcactgta aatatcctgg
     3301 cattagtaat gccaggatat ttacagaaga ggagcataac catgacggaa cggggttaag
     3361 tcaggttgtc cttaacgcta ttttcaacct cgtatgcctt cttcaggttt atgtccagac
     3421 ttcatacctc tctcagcaat cctcaataat cagatacacc gctttcaccg gaccatgaac
     3481 cccgacgact ttgataagct caatgtccgc cgttgagctg ggaccactga tgatgttaat
     3541 acatgacggc atgcgttcac cggcctgcgc tttctgatgc aatctttctg cgagttgcgc
     3601 tacacgcggc agaatagtgc ttttacgcag gataaaaaga gatgattccg ggagcaggct
     3661 caatgaacgc ccacgctcgg cggcggaaaa aagaaccacg cctcccgatt cggttaaacc
     3721 atattcagca tacacgacac ccactttagc ctgctctgcc tgcgagatat tctcggcacc
     3781 tttcgccgga tcccaaacaa cggcattgca ttcctgctgc aaacgttcgc taatccctaa
     3841 ttcctccagc ctcgtgtcac cgctaatcac gaccgactga tctcccagct ctttacacag
     3901 tcgtattgca gcttctgccg ccttcgcctc gctggtcagt tcacagcgcg tcaacataac
     3961 atcgctggca aactgaataa acgcgtcaca gcgctgctgt tggttaagtt cggtaagccg
     4021 ctcgttagca tagttgttaa gcggcgcatc ttctgcttgc ggttcaagtc gcagcgggcg
     4081 acccagtgcc tgagcaacgt tattcaaaaa ttcgcctcga ttatccattc tttttctcct
     4141 gcgcctgatg tttcttaaac caactacgga aactctctcc gtcagcttca ggaagatcgc
     4201 gtgcttccat ccagtcgcta atcgcgccaa atttgagtgg tgttttgccg ccattgataa
     4261 accagcttgc cgcatgagcg ccggccatca tcccgacttt ccacaatcct ggatgactat
     4321 tggcataagc gaacattttt atcgcccgtt gctctgcttt tgcggtgatc cctttttcag
     4381 ccatcacccg acgatgacgc aaaatcagtt ttgacagcgg aatacgcact ggacacacgt
     4441 tgtcacaagc tgtgcataaa gagcaggcgt agggtaaatc tttaaaatct ttatagccgc
     4501 caagtagcgg agaaatcacc gcaccaattg gccctggata aatagagcca tatccatgac
     4561 cgccaatatg gcgatatgcc ggacaagtat tcatacaagc cccgcagcga atacagcgca
     4621 gcacatcccg aaattcagag gccagcacct cagaacgccc gttatcgaca ataaccagat
     4681 gaaactcttc aggaccatca acgtgccccg cttcgcgcgg tcctgtcagc caggtgttgt
     4741 atcccgtcaa acgtgcacca acggcactgc gcgccagcat ggtgatcaat acatctacct
     4801 cggcaaacgt gggggcaata cgctccattc ccatcactgc aatatgcgtt ttaggcagcg
     4861 tggtacacat tcgcgcatta ccttcattgg tcaccaggca taccgaacct gtctctgcca
     4921 ccgcgaaatt acagccggta atacctattt cagcactgag gaaatcttcg cggatttttt
     4981 gccggatgaa taaggtcatc gcttcaggcg tttccggccc ctcatagccc agacgttcgt
     5041 gtagcactcg acggatctga tggcgatctt tatgaattgc cgggaccaca acatgagatg
     5101 gcggatcttg atccagctgg agaatatatt cacccagatc ggtttcaatc acctgaatgc
     5161 cagcatcctg caacacatga ttgacaccaa tctcttcggt caccatcgat ttagatttca
     5221 ccaccttccg ggcatttttg cgttgggcaa cctgtaaaat gtagcgggta gcgtcttctt
     5281 tggtttttgc aaaatagacg tgaccgccgt tttgcgtcac tttttctgag agctggtaca
     5341 gataagcgtc gagattactc agaacatgat cacgtatctg ggcggcccga tcgcgccact
     5401 cctcccagtg ccccaattca tcgaccattt tttgccgatt tgccccaata cgctgctgcg
     5461 cgtttgccac cgctttgcgc atgatcggat cttcaatttg ctgacggatg cgtgtcttaa
     5521 aatctgtatt actggttttg atcgacatct ttatatcctc agcggctcat caacacttca
     5581 gcaatatgca tcactttgac tttctgccct tcccgttgta atcgcccact gatgttcagc
     5641 aggcaactca cgtcagcacc tattaaatac tcagggcgga cttccatcag gtgcgcaacc
     5701 ttttctttca ccatctcgcc ggatatttcg gccattttga ccgagaacgt gccgccaaat
     5761 ccgcagcagg tatcctgttc agcaaaggtc aacagctcca gtccacgcac atttttcagc
     5821 agcgtaagtg gctcgtcctt cactcccagc ttacgggcca ggctacaaga tgggtgatac
     5881 accgctctcc cttgcaaact ggcacctaca tcgactaccc ctaatttatt aacaataaaa
     5941 gaggtgagat cctgcatacg cgcggcaacc ttttcggctc gtgatgccca ttcaggttca
     6001 tccgccagat acgtcgggta actttttacg gcataggtgc aagagccagc tggtgaaata
     6061 atgggatcgt cgttatcctc cagtgcggcg atcagatttt tcatccctgg aatcgcttct
     6121 ttgatataac cactattgat cgcaggctga ccgcagcatc cctgtttctc cgggaaattt
     6181 acgcgacagc cgagtttttc cagtagcagc acggagtctc gtgccattct tgatttcagg
     6241 gcgtcaccaa tacaggtgac aaagaaattg acattcacaa ctaatactcc attacttcat
     6301 gcccatttat gcccgatgtt tcccccctcc gggggatcaa tcatcaataa atatggcgtt
     6361 cttttttaat tggctagcaa catactaccc ctcatttgta tgacaaatga taatctttca
     6421 tccaaacttc aaatgcaata actaatttta tttctttcgg catcgctcac attttatggg
     6481 gaaatcgacg atcatttttg gcataagaaa tccatttaaa aatggctaca tcccttatac
     6541 ctattcagag ctacttattc aggggttatt gagatataca ctgatggtgt acaaaaaaat
     6601 caccgacaat aatcacgtat caagtaatag tcgggatttt atttaattga agcaatttag
     6661 tttgcatttt tatttaaaaa ctgagctaga gtccatcaga aaaaaaatat ataaagccca
     6721 aaccaccgtt ggaaatagaa atagttgaaa ggctcacaac tcccggaatg cggattatct
     6781 ttgccctgat ttctggcggt tttattcagg gtgcaagctg tctgaccctt tcccgatatt
     6841 ctcccggggt acaaccaaac tcgcgaacaa acgccttgtg aaaagatgat tcactggcat
     6901 agcctactga ctcagcgatc accacaacag ggagcatttc ccgggaaaac atctgggccg
     6961 ctatttgtag acgcaacttt gttaatacag ccagcggcgt ggttccggaa acatcacgga
     7021 aaagctgggc aaaatttgcc cgggacatgt gggcgatgct ggccagcgat tcgacggtcc
     7081 aggcgtgtcc tggcatttcc agcatttgct gtattaccgc accaagacgt ggatgcagaa
     7141 gcaaactgag aatgtttttc tcagtattaa cctgtgcaat ccactcacgc accgcgaggg
     7201 taaagaatgt agcgcagatc tggctacaca gagcatccat tcccggcatt gccgatctgg
     7261 attcctgttg taaaaacggg atcgcctccc tcagccagtt atattccacg ctgtggttaa
     7321 ccggtgctaa aaacagcgtt tccggcaaag acgttaaaaa ataacgcgcc gaatgttgca
     7381 accgaagagt gccgcagaca atacaggtcg attcattatc gacatgactc aggcgatgag
     7441 ctgaattttg tggtagcagg accacatttc ccgggcgtaa tgtaaaaatc tctcccgtcg
     7501 gcatttccag cttcgccgct ccttgcgtta acgcatgcca acgaataacc gataattccc
     7561 cggcaccatg tggaagctgc cagtcacttc ctaacacgca attcttatcg atcgttcctt
     7621 gtggagcgtt aagcatcaac aaacggctaa gggcatccat atcagactcc tgagtggttt
     7681 ttgattagaa gcaccaacaa tcttagtaaa taactaatct aataccatca aatagttaat
     7741 catgattttt gttttgtgat tctgattaag aaaaagaaaa taccagacga taagacaaaa
     7801 tatcaagcgt tgtgaagaaa tgttatttgc tcttttgcgt ctatagtcat gatgtcaaat
     7861 gaacacgttt cgacaggaaa tcatcatgaa taaatatcag gcagtgatta ttggttttgg
     7921 caaggctgga aaaacattag ccgtcacgct ggcaaaagca ggttggcgag tggctctcat
     7981 cgaacaatca aatgcaatgt atggcgggac ctgtattaat atcggctgca tcccaaccaa
     8041 aacattggtt catgacgcac agcaacacac agattttgtc cgtgccatac agcgtaaaaa
     8101 tgaagtggtt aattttttac gtaataagaa ttttcataat cttgcagata tgcccaatat
     8161 cgacgtgatc aacggccagg cggagtttat caataatcat agcctgcgtg ttcatcggcc
     8221 tgagggaaat ctggagattc atggcgagaa aatttttatt aatactggtg cacaaaccgt
     8281 ggttccgcca attcctggaa ttaccaccac gcctggtgta tatgacagca ccggattact
     8341 taatctaaaa gaattgcctg ggcatttagg tattttgggc ggcggatata ttggcgttga
     8401 gttcgcctct atgttcgcta attttggcag caaggtaacc attttagaag cagcttcgct
     8461 gtttttgcct cgggaagaac gggatattgc tgataatatc gcgatgattt tacgcgatca
     8521 gggcgtcgat attatcctca atgcccatgt ggagcgaatc agtcaccatg aaaatcaagt
     8581 gcaagtgcat agcgagcacg cccaactggc ggtggatgca ctgttaatag cttccggtcg
     8641 tcaaccggct accgcttcgt tacatccaga aaatgccggt atcgcagtaa acgagcgcgg
     8701 ggcaattgtc gttgacaagc gattacatac caccgcagac aatatttggg cgatgggaga
     8761 tgttaccggc gggctgcaat ttacttacat atcactggat gattaccgca ttgtacgtga
     8821 tgagttactg ggtgaaggca aacgtagtac tgatgatcgg aaaaatgtgc cttattccgt
     8881 atttatgaca ccgcccctgt ccagggttgg tatgacagaa gaacaagcca gagagagtgg
     8941 tgctgatatt caggtggtga cattgcctgt agctgcaatt ccgcgcgcca gagtgatgaa
     9001 cgatacccgt ggggtattaa aagcgattgt tgataataaa acccaacgta tattaggggc
     9061 atcactgctg tgtgttgact cccacgagat gattaatata gtgaaaatgg tgatggatgc
     9121 cgggctgcct tatagcatat tacgcgatca gatatttact catccgtcga tgagcgaatc
     9181 actcaatgat ctattttcat tagtcaaata aactcaaaat cagacgccag aacaaatatt
     9241 ctggcgtctc agagaaaaga atcttattaa ttccctgtta ctctataact
//
LOCUS       DAZFFG010000156         9225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFFG010000156 DAZFFG010000000
VERSION     DAZFFG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.157"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            131..1528
                     /gene="ulaA"
                     /locus_tag="ACHJ7J_004401"
     CDS             131..1528
                     /gene="ulaA"
                     /locus_tag="ACHJ7J_004401"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5002544.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1544..1849
                     /gene="ulaB"
                     /locus_tag="ACHJ7J_004402"
     CDS             1544..1849
                     /gene="ulaB"
                     /locus_tag="ACHJ7J_004402"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO5002545.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQYLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1859..2323
                     /gene="ulaC"
                     /locus_tag="ACHJ7J_004403"
     CDS             1859..2323
                     /gene="ulaC"
                     /locus_tag="ACHJ7J_004403"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO5002546.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2337..2987
                     /gene="ulaD"
                     /locus_tag="ACHJ7J_004404"
     CDS             2337..2987
                     /gene="ulaD"
                     /locus_tag="ACHJ7J_004404"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5002547.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2997..3851
                     /gene="ulaE"
                     /locus_tag="ACHJ7J_004405"
     CDS             2997..3851
                     /gene="ulaE"
                     /locus_tag="ACHJ7J_004405"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO5002548.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3851..4537
                     /gene="ulaF"
                     /locus_tag="ACHJ7J_004406"
     CDS             3851..4537
                     /gene="ulaF"
                     /locus_tag="ACHJ7J_004406"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO5002549.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGQAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4666..4941)
                     /gene="yjfY"
                     /locus_tag="ACHJ7J_004407"
     CDS             complement(4666..4941)
                     /gene="yjfY"
                     /locus_tag="ACHJ7J_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO5002550.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5268..5663
                     /gene="rpsF"
                     /locus_tag="ACHJ7J_004408"
     CDS             5268..5663
                     /gene="rpsF"
                     /locus_tag="ACHJ7J_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO5002551.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5670..5984
                     /gene="priB"
                     /locus_tag="ACHJ7J_004409"
     CDS             5670..5984
                     /gene="priB"
                     /locus_tag="ACHJ7J_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO5002552.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5989..6216
                     /gene="rpsR"
                     /locus_tag="ACHJ7J_004410"
     CDS             5989..6216
                     /gene="rpsR"
                     /locus_tag="ACHJ7J_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO5002553.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6258..6707
                     /gene="rplI"
                     /locus_tag="ACHJ7J_004411"
     CDS             6258..6707
                     /gene="rplI"
                     /locus_tag="ACHJ7J_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO5002554.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6778..7572)
                     /gene="yjfZ"
                     /locus_tag="ACHJ7J_004412"
     CDS             complement(6778..7572)
                     /gene="yjfZ"
                     /locus_tag="ACHJ7J_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5002555.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLAKKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            7845..8138
                     /locus_tag="ACHJ7J_004413"
                     /pseudo
     CDS             7845..8138
                     /locus_tag="ACHJ7J_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(8196..8623)
                     /gene="tnpA"
                     /locus_tag="ACHJ7J_004414"
                     /pseudo
     CDS             complement(8196..8623)
                     /gene="tnpA"
                     /locus_tag="ACHJ7J_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
     gene            8631..>9225
                     /locus_tag="ACHJ7J_004415"
     CDS             8631..>9225
                     /locus_tag="ACHJ7J_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5002556.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVF"
BASE COUNT         2276 a         2192 c         2449 g         2308 t
ORIGIN      
        1 ggaattattt gtgtgtcgcg tcacatttaa tcataaataa tcttgttgtg attacttttg
       61 aaaattagag tgagtgcaca acattccggg tgtgaggaat acccggttac ctcttcttca
      121 ggagatcgtt atggagatcc tctacaacat ctttaccgtg ttttttaacc aggtcatgac
      181 caatgccccg ttgttgctgg gtattgtgac ctgtctgggc tacatcctac tgcgcaaaag
      241 tgtcagcgtt attattaaag gcacgattaa aaccataatt ggtttcatgt tgttgcaggc
      301 agggtccggc atcctcacca gcaccttcaa accggtggtg gcgaaaatgt ccgaagtcta
      361 cggcattaac ggcgcaattt cagatactta cgcttcgatg atggcaacca tcgaccgcat
      421 gggcgatgcc tatagctggg taggttacgc ggtgttgtta gcgctggcac tgaacatctg
      481 ttacgtgctg ctgcgtcgca ttaccggtat tcgtaccatc atgctgaccg gccacattat
      541 gtttcagcag gcggggctga ttgccgtcac gctgttcatc tttggctact ccatgtggac
      601 caccattatc tgcaccgcga ttctggtttc gctctattgg ggcattacct ccaacatgat
      661 gtacaagccg actcaggaag tcaccgacgg ctgtggtttc tccatcggtc accagcaaca
      721 gtttgcatca tggattgcct ataaagttgc gccgttcctc ggcaaaaaag aggaaagcgt
      781 tgaagacctc aaactgccag gctggctgaa tattttccac gacaacatcg tctccacggc
      841 gattgtcatg accatcttct ttggtgccat tctgctctcc ttcggtatcg acaccgtgca
      901 ggcgatggcg ggcaaagtgc actggacggt ctacatcctg caaaccggtt tctccttcgc
      961 ggtggcgatc ttcatcatca ctcagggcgt gcgcatgttt gtggcggaac tctctgaagc
     1021 atttaacggt atctcccagc gcctgatccc aggtgcggtt ctggcgattg actgtgcggc
     1081 tatctatagc ttcgcgccga acgccgtggt ctggggcttt atgtggggca ccatcggtca
     1141 gctgattgca gttggcatcc tggtcgcctg cggctcttcc attctgatta ttccaggctt
     1201 tatcccgatg ttcttctcca acgccaccat cggcgtgttc gctaaccact tcggcggctg
     1261 gcgtgcagcg ctgaagattt gtctggtgat gggaatgatc gaaatctttg gttgcgtctg
     1321 ggcggtgaaa ctcaccggta tgagtgcctg gatgggcatg gcggactggt cgattctggc
     1381 accgccgatg atgcaaggct tcttctccat cggtatcgcc tttatggccg tcatcattgt
     1441 aattgcactg gcttatatgt tcttcgctgg ccgcgcgctg cgcgcagaag aagatgcaga
     1501 aaaacaactg gcagaacagt ctgcttaata aggagttttg attatgaccg tacgtattct
     1561 ggctgtgtgt ggcaacggac aaggcagttc catgatcatg aaaatgaaag tggaccagta
     1621 tttaacccaa tcaaacattg accacacggt aaacagctgc gcggttggcg agtacaaaag
     1681 cgagttgagc ggcgcggata tcatcatcgc ttctacgcac atcgcgggcg aaatcaccgt
     1741 gaccggcaac aaatatgtgg ttggcgtgcg caacatgctc tctcctgccg attttggccc
     1801 gaaactgctg gaagtgatca aagaacattt cccgcaggac gtgaagtaag gacgcgcaat
     1861 gaaattacgt gattcgctgg cggagaataa atctatccgc ctgcaggctg aagcagagac
     1921 atggcaggac gcagtaaaaa tcggcgttga cctgctggtg gcggcggatg ttgtcgagcc
     1981 gcgttactac caggcgattc tggatggcgt ggaacagttt ggcccttatt tcgttatcgc
     2041 tccggggctg gcgatgccgc acgggcggcc ggaagagggt gttaagaaaa caggtttctc
     2101 tctggtaacg ctgaaaaagc cgctggaatt taatcacgac gacaacgatc cggtggatat
     2161 cctcatcacc atggcggcgg tcgatgccaa tactcaccag gaagtgggca tcatgcagat
     2221 cgtcaacctg tttgaagatg aagagaattt tgaccgttta cgcgcctgcc gtaccgagca
     2281 ggaagtactg gatctcattg accgcaccaa cgcggcagct taagaaggaa ttgaacatgt
     2341 cattaccgat gttgcaagtc gcgctggaca accagactat ggatagcgcc tacgaaacca
     2401 ctcgcctgat tgccgaagaa gtcgacatta tcgaagtggg taccattctg tgcgtgggcg
     2461 aaggcgtgcg tgcggttcgc gatctgaaag cgctttaccc gcacaaaatc gtactggcag
     2521 acgccaaaat tgccgatgcg ggcaaaatcc tttcgcgtat gtgcttcgaa gccaacgctg
     2581 actgggtgac ggtaatttgc tgtgcggata tcaacaccgc caaaggcgcg ctggacgtgg
     2641 caaaagagtt taacggcgac gtgcagatcg aactgaccgg ttactggacc tgggaacagg
     2701 cgcaacagtg gcgcgatgca ggcattcagc aggtggttta tcaccgtagc cgtgacgcgc
     2761 aggccgcagg cgtggcgtgg ggcgaagcgg acatcaccgc gatcaaacgt ctttccgata
     2821 tgggcttcaa agtcaccgtc accggaggcc tggcgctgga agatctgccg ctgttcaagg
     2881 gtattccgat tcacgtcttt atcgcgggcc gtagtatccg tgatgccgct tctccggtgg
     2941 aagccgcacg tcagttcaaa cgttccatcg ctgaactgtg gggctaagga gcggatatgt
     3001 tgtccaaaca aatcccgctt ggcatctatg aaaaagcgct ccccgccggg gagtgctggc
     3061 tggaacgcct gcaactggca aaaacgttag gcttcgattt tgtcgagatg tcggtggatg
     3121 aaacggacga gcgcctgtcg cgcctcgact ggagccgcga gcagcgtctg gcgctggtca
     3181 atgcgattgt tgaaaccggc gtgcgcgtgc cgtccatgtg cctttctgct catcgtcgtt
     3241 tcccgctggg cagtgaagat gacgcggtgc gggcgcaggg gctggagatt atgcgtaaag
     3301 ctatccagtt cgcccaggat gtcggtattc gcgtgatcca gctggcgggc tatgacgttt
     3361 actatcagga agccaataac gaaacgcgtc gtcgtttccg tgacggcctg aaagagagcg
     3421 ttgagatggc aagccgcgcg caggtgacgc tggcgatgga gatcatggat tatccgttga
     3481 tgaactccat cagcaaggcg ctgggctacg cgcactatct caacaatccg tggttccagc
     3541 tctacccgga tatcggcaac ctgtcggcgt gggataacga cgtgcagatg gagttgcagg
     3601 ccggaatcgg gcatatcgtc gcggtgcatg tgaaagacac caaaccgggc gtcttcaaaa
     3661 acgtgccgtt tggcgaaggt gtagtggatt tcgaacgttg tttcgaaacg ctcaaacaga
     3721 gtggctattg cgggccgtac ctgattgaga tgtggagcga aacggcggaa gacccggcgg
     3781 cagaagtggc gaaagcgcgt gattgggtga aagcgcgcat ggcgaaagcg ggcatggtgg
     3841 aggcggcata atgcaaaagc taaaacagca ggtgtttgaa gccaacatgg atctgccgcg
     3901 ctacgggctg gtgaccttta cctggggcaa cgtcagcgcc attgaccgcg aacgcgggct
     3961 ggtggtgatc aagcccagcg gcgtcgccta cgaaaccatg aaagcagacg atatggtggt
     4021 ggttgatatg agcggcaagg tggtggaagg ggagtatcgc ccatcttccg acactgcgac
     4081 gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc attgtccata cccactccac
     4141 tcatgccacc gcatgggcgc aggcggggca ggcgatcccg gcgttaggca ccacgcacgc
     4201 tgactacttc tttggcgaca ttccgtgtac gcgcgggtta agcgaagaag aggtgcaggg
     4261 cgagtatgaa ctgaacaccg gcaaagtgat tatcgaaacg ctgggcaacg ccgagccgct
     4321 gcatacgccg ggaattgtgg tgtatcagca cggaccgttc gcctggggga aagatgccca
     4381 cgatgcggtg cataacgcgg tggtgatgga agaagtggcg aaaatggcgt ggattgcgcg
     4441 cagtattaac ccacaactca atcacatcga cagcttcctg atgaataaac acttcatgcg
     4501 taaacacggt cctaacgctt attacgggca gaagtagaac acgcgctgcg gaaatttcct
     4561 tcctcgggag ataactggtc taattccgca gccgtttttc aaaaaaagcc ccctgcgaag
     4621 ggggcaaagc aaactatggc aatgtttcgt tggttatacc tggtgctagc gataaatatc
     4681 cgcgctggcg tgcatattgc cgttactccc cggttcccgc atcagaatta cgtggtagta
     4741 cgttgcgcct tgcgcatctg tttcttcatt taatgcctga cgtgcttcgc tttcagtggc
     4801 gaagttatga ttgatataaa tcacgcctaa gctttgcaca tcgtccatat ttctggcctg
     4861 gtggttatta atttcaatgg ctgcccatgt atttgcactt agcaaaagca cagccagaag
     4921 ggctaaaaca cgactgaaca tagatacctc ctcgacggct gactttgtgt gctctcctct
     4981 gtgatgatct tctgatttaa ttttaatcaa tgataaagaa gttgatggtg actatttctg
     5041 atgcagttgt tcaaaaaaac accatgatga agtgtgatga acttcaaatc agcgtgttag
     5101 tccactttgg tgaaagatga gatttatttg tcggctggcc ttgagtttag cggggtatgt
     5161 cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc tccccgggat
     5221 tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg cgtcattacg
     5281 aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg atcgagcgct
     5341 acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac tggggccgcc
     5401 gtcagctggc ttacccgatc aacaaactgc acaaagctca ctacgttctg atgaacgttg
     5461 aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac gatgccgtta
     5521 tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg atggttaaag
     5581 cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct gatgatgctg
     5641 aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg tccggcaccg
     5701 tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc cagttcgtgc
     5761 ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg gcaggcatgg tgtcaaatgc
     5821 ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg gtcggcagtc
     5881 gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg agcaaaatgg
     5941 ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat ggcacgttat
     6001 ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat cgactataaa
     6061 gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc aagccgtatc
     6121 accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg cgctcgctac
     6181 ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca ttaatacgac
     6241 tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac ctgggtagcc
     6301 tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta ccgcagggta
     6361 aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc gctgaactgg
     6421 aagctaaact ggctgaagtt ctggcagcag ctaatgctcg cgctgagaaa atcaatgcac
     6481 tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc ggttccatcg
     6541 gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct aagagcgaag
     6601 ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc ttccaggttc
     6661 acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc gttattcaac
     6721 gagacgtaaa aacgccgacc attggtcggc gttttgcttt ctatttttcg tcaggtatta
     6781 gtttcgcaag tagatccatt caccattctc ttgtgtccat aagtcaggat ggtttgctgg
     6841 cgttagatac atacgagcgc aactgatgtc tcgctcctgc ggcactcgct cgaaacccaa
     6901 acccgagtag attgcggctg aacttgataa ggaacgcaga ccaattttca aatcatgggg
     6961 taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat tagaaacgcc
     7021 tgtaacagtg cgaagttggt cgatataaag atcgattgtt ggtagttcag atgaccagca
     7081 attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg tatagtctgg
     7141 agtaccataa ccaggtccca aaaccacttc gtgtattgtg tatttactgt tgggttcgaa
     7201 ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac ccgcgtaagc
     7261 tttattgtat ttctgggcgt gaatattatc taatgtagct tcatggttcc atccaaaaga
     7321 attatcacca ttcattgtta ctccatcgta agcattacac ttctttgcta acttattgaa
     7381 tgcttgccag tcagagagat ctagtttgtg gatctcaaca gtacgtctgc catcgcaatt
     7441 atgatctctt ccacgatttg acagtaatgt agcgcgtgcg tattgatgaa attttagttt
     7501 tgctggtttg tcggtggacg aaaaacgagg taaatatgcg gggaatgaat aaatagttgt
     7561 tggtaatgtc attttattgt tgcctgttta atatttttac aaatatcgca gttgaatgtt
     7621 caaagaaaaa gaattgatat taataaaaat aagtttgttt tctgaagtgg tgaaaatagc
     7681 ggatggtgaa agatattaat atcaaaaatc gctcattatg gatataacga tgcacccggg
     7741 gagaaaatga cttccacgcc ttacaccgta cattcttgtt gtgcggtatg taattattct
     7801 acgctgtcgg tatcggtgta ttcgttaatc agcaggttag ccagccacaa aaagccattg
     7861 agaaaattat tgattttaca tgggattatt atattgctaa tccctggttt ttaaaaattg
     7921 tgcatagtga aaatcaaagt aaaggtgtgc attatgcaaa atcacagcgt ctcttagaaa
     7981 ttacccatgc gcacttgcag ttaatggaat ccttattgga tgaagggaag aaacacaata
     8041 tttttaagcc agatatcgac ccatttcagg tgaatattaa tattgctgcg cttgggggat
     8101 attatttgat caaccagcat acgcttggcc tggtttatcc ggtgcgccgt aaaaccccgt
     8161 ccttcagggc ggggatatac ggcgcggttt tccacctaac caggtgtttg ctgttgttca
     8221 atgtattgac ggatggcgga tattggcgca cctccgcagc tactggcaaa atagccagga
     8281 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
     8341 aacctacccg atacgccttt gaggctgttt accagactgg atatcgccag tttgggagga
     8401 tagttaatta acaaacgtga tctggttcgc catccatttc aaccagttca gcttcaaaat
     8461 cagcacatac ctttgaaaag taagtgcgta gtttttctgt cgcgtcatgg tcaaaaatct
     8521 ggcgtcggta tctggtgaca aagaccaggt gaacatgcat caggaaaaca caatgccttc
     8581 cacgccggat atcggtttct tttttcacag accaaagtat catttggcct gtgaaacgac
     8641 tacaggcatt taaattccag ttaagacccg gtggtcaaca ggagcgtgaa atgaggcgct
     8701 tcgcaggcgc atgtcgtttc gttttcaatc gtgctctggc gcttcagaat gagaatcatg
     8761 aggccggtaa taaatacatc ccttacggga aaatggcttc ctggctggtt gagtggaaaa
     8821 atgccactga aacgcaatgg cttaaagatg ccccgtcaca gccattgcaa cagtcactga
     8881 aagaccttga gcgggcttac aaaaacttct tccggaagcg ggctgctttt ccccgattca
     8941 aaaagcgggg acagaatgat gcattccgct acccgcaggg cgttaagctc gatcaggaaa
     9001 acagtcgtat ttttctgccg aaacttggct ggatgcgcta ccggaatagc cgtcaggtca
     9061 cgggtgttgt gaaaaatgtc actgtcagcc agtcctgcgg taagtggtac atcagtattc
     9121 agactgaaaa tgaagtatca actccggttc acccttcagc attaatggtc ggactggatg
     9181 ctggcgtggc taaactcgcc acgctgtcag atggcacagt ctttg
//
LOCUS       DAZFFG010000157         9103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFFG010000157 DAZFFG010000000
VERSION     DAZFFG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.158"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..785
                     /locus_tag="ACHJ7J_004416"
     CDS             <1..785
                     /locus_tag="ACHJ7J_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002557.1"
                     /translation="KQTQFINASQFDINELQQSSAFLASKYHTIDIQEKTDAFVNEFK
                     GILFDQNGRSSEFLLNFYECCYKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNIG
                     FDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFF
                     SGQNFSNYDIQYVNWGTSLFDVDTPCIFNTPANHESYEKSLKPVSENGLNGVLSDRNK
                     KIKMITGVAPFDDILFMDDDFDDNSPEDAPIENSPVVNSPLV"
     gene            1155..1313
                     /locus_tag="ACHJ7J_004417"
                     /pseudo
     CDS             1155..1313
                     /locus_tag="ACHJ7J_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1537..1656
                     /locus_tag="ACHJ7J_004418"
     CDS             1537..1656
                     /locus_tag="ACHJ7J_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5002558.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            1721..6295
                     /gene="ypjA"
                     /locus_tag="ACHJ7J_004419"
     CDS             1721..6295
                     /gene="ypjA"
                     /locus_tag="ACHJ7J_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO5002559.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPGMAAFTSDVIGVVNDETV
                     DGTQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTTIESGNQDVYKGGISNGTTIKGGNSHISGGTANGTIIDGGSQ
                     RVTTQGHVDSTTINKSGSQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALNNTIEGGTQSLNSKSTAKNTQIYAGGTQIVDNTSTSDVIEVYSGGVLDVRGGT
                     ATNITQHDGAALKVTTYDLTVSGTNSEGAFSIHNKVADNVLLENGGHLEVSHSANKTI
                     IKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNINSG
                     TQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETGSA
                     LVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGKLI
                     VQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDAFY
                     IRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIYAS
                     ATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAINTD
                     IVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQSS
                     QGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNTGG
                     QEIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTV
                     NDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNALLENGGNLLVLA
                     GTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQPLARAEDLQVAGGTAI
                     VYAGTLADASISGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLADLT
                     AASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTTNE
                     LSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKAGGGDA
                     SFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPIPNPKPDPKPDPKPDPNP
                     KPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMKSKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     TLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6617..6937)
                     /locus_tag="ACHJ7J_004420"
     CDS             complement(6617..6937)
                     /locus_tag="ACHJ7J_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5002560.1"
                     /translation="MHILSVPTTVPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFH
                     DDAAIEEHIEAGITLADAVNFLVEKYELVRIDRKGFSWQDQTSFLSATDILRARRATG
                     IMST"
     gene            complement(6969..7289)
                     /locus_tag="ACHJ7J_004421"
     CDS             complement(6969..7289)
                     /locus_tag="ACHJ7J_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5002561.1"
                     /translation="MKNSWGLKRDMTPCFGARLVQEGRRLHFLADRADISGAFNEEEA
                     LRLDQAFPLVMKQLELMLTTGELNPRHQHCVTLHHNGLTCEADTLGSHGYVYIVIYPQ
                     TSSQ"
     gene            complement(7307..7528)
                     /locus_tag="ACHJ7J_004422"
     CDS             complement(7307..7528)
                     /locus_tag="ACHJ7J_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO5002562.1"
                     /translation="MKIIDKRAAMAIQRQHPDSRIFRYCTGKYQWHGSASHYTGQDIA
                     EISGVLAVYAERRCDNHGPYTRLMCITTN"
     gene            complement(7536..8009)
                     /gene="radC"
                     /locus_tag="ACHJ7J_004423"
     CDS             complement(7536..8009)
                     /gene="radC"
                     /locus_tag="ACHJ7J_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5002563.1"
                     /translation="MSEINLPHDLPVTEARTIRRALRLLEKYQRQPGEQFNSTTFTKT
                     WLQLRLALQEREVFIVLYLDNQNRLLQCETLFTGTINHTEVHPREVVKLALRHNAAAV
                     ILAHNHPSGETEPSKQDQHITQHLVKALAIVEVRVLDHLIVGIGDVLSFAERGWL"
     gene            complement(8042..8860)
                     /locus_tag="ACHJ7J_004424"
     CDS             complement(8042..8860)
                     /locus_tag="ACHJ7J_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5002564.1"
                     /translation="MRLASRFGAVNLVRRDRPLTRDELAHYVPSVFSEEKHESRSERY
                     TYIPTITLLDNLQREGFQPFFACQTRVRDQSKREHTKHMLRLRREGQITGKQVPEIIL
                     LNSHDGSSAYQMLPGLFRAVCQNGLICGESFGEVRVPHKGNVVEKVIEGAYEVLGIFD
                     RVEEKRDAMQSLLLPPPAQHAMAKAALTYRFGEEHQPVTESQILSPRRWQDESNDLWT
                     TYQRIQENLIKGGLSGRTTKGRRAQTRAVKGIDGDVKLNRALWVMAENMLQLAS"
BASE COUNT         2516 a         1983 c         2284 g         2320 t
ORIGIN      
        1 taaaacaaac gcagtttatc aacgctagtc aatttgatat taatgagctt cagcagagtt
       61 ctgctttttt agcgtcaaaa tatcacacca tagatattca agaaaaaaca gatgcgtttg
      121 tgaatgagtt caaggggata ttatttgatc aaaatggaag gtcttcagag tttctactta
      181 atttttatga gtgttgctat aagtttttac caagagctca gcctcaagat aaaatcgata
      241 gctataattc agcactgcaa gctttttcca tattttgttc atctacgttg acacataata
      301 atataggctt tgatttcaaa ttatttccag aagtcaagct gtctggagaa catcttgaaa
      361 cggtattcaa atacaaaaat ggcgatgatg tccgggagat agccaaaatt aacattactc
      421 tccaaaaaga agagggtggc ttatataatc tacgtggatt ggattttaag ggatgcttct
      481 tttctggaca gaacttcagt aactatgata ttcaatatgt gaactgggga acgtcattgt
      541 ttgatgttga tactccgtgt atttttaata cgcctgctaa ccatgagagt tatgaaaaat
      601 cattaaaacc tgtgagcgaa aacggtttaa atggagtctt atctgatcgt aataaaaaaa
      661 taaaaatgat cacgggtgtg gcaccattcg atgatatttt atttatggat gatgactttg
      721 atgataactc ccctgaggat gctcccattg agaatagtcc tgttgtgaat agtccccttg
      781 tatgaagttc taaaagtagt tttcaatgat aaataaatgg ctatttttac gaagagataa
      841 taacgtagat tgaaacaata tgtattattg attgagtaaa tgggattatt cttcacacta
      901 catccatttt agtgtcacta ccaaagaaaa aggggctacg ttttcatgta accccttgat
      961 ttatttggtg ggctagcagt gactgaattg gtgctgtaat tggttgaaat atttcatcta
     1021 atgtctggtt caactttcct tagctgcctg aaagtttttt gatctcaccc agcaataaca
     1081 gcgttacggt gcaccacgcc tgactgaaga actgcgtgct cagggttacc acttcaacgt
     1141 aaaaccgtgg caggaggtcc ggactgaaaa aactggtcag gacatcatcg gcagggaacg
     1201 cactgctagt ctgtaaactg aaccgactgg ggcgcagtat gtggcatctt gtcgtgctgt
     1261 tggaggatct gtgcgaacgt ggtattaact ttcgtgctct ggcccaatct atatttgcca
     1321 aacaatgggg ggacgaatgc tgtaaaagta aaacaatctg cgatctcaaa gttattgtgt
     1381 gatttttatg tgagcagaag atattcatca gaaacgatta tgtaaatcat tttattttgc
     1441 caacggcctc attgtcgaaa gaaaagaata cgcccgtgtt tcctgaatag attgactttt
     1501 ttatccaacc acacttcagc gcactgcgtt taaaaaatgc ctcattctta tgcggaatat
     1561 catcatttca tcatgatgtc tttgatgagc ggtgaacaca atacacttgc gctgtctctt
     1621 caggatgaat cccctggtct ggtgcctgtg ggctgatgtt gcagcaaagc taaggtcgct
     1681 taaacgctac tcagtattca cttttcagag gatgaaattt atgaacagga ccagtcccca
     1741 ttattgtcgc cgctcagtac tttccttatt gatatctgcc ttgatatatg ccccgcccgg
     1801 gatggcggcc ttcacttctg atgttattgg tgtggtaaac gatgagactg tagatggcac
     1861 ccaaagagta gatgaacgag gtacaacaaa taacactcat attatcaacc atggccagca
     1921 gaatgttcat ggcggggtat ctaatggaag tcttattgaa tctggtggat atcaagatgt
     1981 aggaagtcat aacaattttg tggggcaggc taataataca accattaacg gtggcagaca
     2041 gtcaattcat gacgggggta tttccacagg tacgacaatc gagagtggca atcaggacgt
     2101 ttataaaggt ggtatcagca atggaacgac aattaagggt ggtaattcac acataagtgg
     2161 ggggactgcg aatggaacaa tcattgatgg tggcagccaa cgagtaacaa ctcaggggca
     2221 tgtcgacagt acaacgataa ataagtctgg ctctcaggac atcacgcaag gaagtctggc
     2281 aacgaacaca accataaatg gtggtcgaca gtatgttgaa cagagcacag tagaaacaac
     2341 caccattaaa aatggcggtg agcaaagagt atatgagagc cgtgcgctga acaacacgat
     2401 tgaaggcgga actcagtctc tgaatagtaa gtcaacggca aaaaatacgc agatctatgc
     2461 tggtggcacg caaattgttg ataacaccag cacctcggat gttattgaag tttattccgg
     2521 cggcgtgctt gatgttagag gtggtacggc aactaatatt actcagcacg atggagccgc
     2581 tttaaaagta acgacttacg atttgacggt gagcggtacg aatagtgaag gggcattctc
     2641 catccacaat aaagtggctg acaatgtgtt gctggaaaac ggaggccatt tagaagtatc
     2701 tcattctgca aacaagacga ttatcaaaga taaaggaaca atgtcagttt taaccaatgc
     2761 taaagctgat gcgacccgaa tagataatgg cggggttatg gatgttgccg gaaacgcgac
     2821 aaataccata attaatggtg gcacacagaa tattaataat tatggcatag ccacaggcac
     2881 caatatcaac agcggaacgc aaaatatcaa aagcggcggg aaagctgaca caacaattat
     2941 atcctccggg agccggcagg ttgttgagaa agatggtacg gcaattggca gcaatattag
     3001 cgccggaggc tcgctgattg tctataccgg cggtattgca catggggtta accaggagac
     3061 gggcagtgct ttagttgcca acacgggtgc agggactgat atcgaaggat acaacaagct
     3121 ctctcacttc actattaccg gaggggaggc taattatgtt gtgctggaaa ataccggcga
     3181 actgacggta gtggctaaaa cctcggcgaa aaatactacc attgatgctg gcggtaagct
     3241 gattgtccag aaggaggcta aaacagatag caccagactt aataatggcg gcgttctgga
     3301 ggttcaggac ggtggtgagg ctaagcatgt tgagcaacaa tccggcggcg cattaattgc
     3361 ttccacgacc tccggaacac ttatcgaagg aaccaacagt tatggtgatg ctttctacat
     3421 caggaattca gaagctaaaa atgtagtgct ggaaaacgct ggctcattaa cagtcgtcac
     3481 tggttcccgg gcagttgata cgattattaa tgccaacggc aaaatggatg tttatggaaa
     3541 agatgttggc actgtactca atagtgctgg cacccaaaca atatatgcca gtgccacttc
     3601 tgataaagca aatatcaaag gtggcaagca aacggtatat ggattagcca ctgaggcaaa
     3661 tatcgaaagt ggtgaacaaa ttgttgatgg tgggtcaaca gagaaaacac acatcaatgg
     3721 cggtacgcaa accgttcaga attatggtaa ggcaatcaat accgatatcg tctctggcct
     3781 acaacaaatt atggcaaacg ggacagcgga aggttccatt attaatggcg gttcacaggt
     3841 agttaatgag ggcggtctgg ctgaaaattc ggtgcttaat gacggcggca cactcgatgt
     3901 gcgggagaaa ggcagcgcaa cggggataca gcagagtagc cagggcgctt tggttgcaac
     3961 caccagggcg acgcgggtca caggaacacg cgcggatggc gtcgcgttca gcatcgagca
     4021 gggtgcggcg aacaatatcc tgctggcaaa tggcggcgtg ttaaccgtgg agtcagacac
     4081 ctcttctgac aaaacacagg tcaatacggg cgggcaggag atcgtcaaaa caaaagccac
     4141 tgcgacaggc acgacgctca ccggcggcga acaaatcgtc gagggtgtgg cgaatgagac
     4201 aacgattaac gacggtggaa tacaaacagt ttcggctaac ggagaggcaa taaaaacaac
     4261 gatcaatgaa ggcggtacgc tgacagttaa cgataatggc aaagcaacag atatcgtcca
     4321 gaacagcggt gccgctctcc agacgagcac ggctaacggt attgaaatca gcggtactca
     4381 ccagtacggc actttttcca ttgccggcaa tttagcgacc aatgcgttgc tggaaaatgg
     4441 cggtaattta ttggtattag caggcaccga agctcgcgac tccacggttg gcaagggtgg
     4501 tgcaatgcaa aacctgggtc aggactccgc cacaaaggtt aactctggcg ggcaatatac
     4561 ccttgggcgg tcaaaagatg agtttcagcc tctggcccgg gcagaagatc tccaggtcgc
     4621 tggcgggacg gcaatcgtct acgcaggtac gctggcggat gcatcgatca gtggcgcgac
     4681 aggaagcctg tcgttaatga cgccacggga taatgttacg ccagttaaac tcgaaggggc
     4741 ggtccggatt accgatagcg cgacattaac tctgggaaat ggcgtcgata caacgcttgc
     4801 cgacctgacg gctgccagcc ggggcagtgt ctggcttaac agcaataatt cctgtgcagg
     4861 taccagcaac tgcgaatata gagtaaacag tttgctactc aacgacggtg atgtttattt
     4921 atcagcacaa acagcagcgc ctgccacaac taacggtatc tacaatacgc tgacaaccaa
     4981 tgaactttcc ggtagcggta atttctacct gcataccaac gttgcaggct cccggggcga
     5041 tcaactggtc gtcaacaaca acgccactgg taattttaaa atctttgttc aggataccgg
     5101 cgtcagccca cagtctgacg acgcgatgac gctggtgaaa gcagggggag gggatgcttc
     5161 gtttacgctg ggcaataccg gcggtttcgt tgatcttggg acctatgagt atgtcctgaa
     5221 aagtgacggc aacagcaact ggaacctgac caatgatgtc aaacccaacc cggaccccat
     5281 cccaaatcca aagccagacc caaaacccga tccaaagcca gacccaaatc caaaaccaga
     5341 cccgactccc gatccaacgc cgacacccgt tccggagaaa cgcattacgc cttctacggc
     5401 agccgtactc aatatggctg caacattacc gttggtattt gatgctgagc taaacagtat
     5461 tcgcgagcgg ttgaacataa tgaaagcgag tccacacaac aataatgtct ggggggcgac
     5521 gtataacacc cgtaataatg tcaccaccga tgcgggtgcc gggtttgagc agacgctgac
     5581 cggaatgaca gtggggatcg acagccgtaa tgatattcct gaaggaattg ccacgctggg
     5641 cgcttttatg ggttattccc attcacatat cggttttgat cgcggaggac atggcagtgt
     5701 gggcagttat tctctgggcg gctatgccag ttgggaacat gaaagtggtt tctatctgga
     5761 cggtgtcgtg aagctgaacc gttttgaaag taacgtagcc ggtaaaatga gcagcggtgg
     5821 agccgccaat ggcagttacc acagcaacgg gctgggcggt cacattgaaa ccgggatgcg
     5881 atttaccgat ggtaactgga acctgacgcc gtatgcatcg ttaacggggt tcaccgctga
     5941 taaccccgaa tatcatttat ccaatggcat gaaatcgaaa tcagtcgata cccgcagtat
     6001 atatcgtgaa ctgggggcaa cgctgagtta caacatgcgt ctggggaacg gtatggaagt
     6061 tgagccgtgg ctgaaggcgg ctgtgcgcaa agaatttgtc gatgataacc gggtgaaggt
     6121 gaataatgac ggtaatttcg tcaatgattt gtcgggcaga cgtggaatat accaggcagg
     6181 tattaaagcc tcattcagca gtacgttaag cgggcatctc ggggtggggt atagccatgg
     6241 tgccggtgtg gaatccccgt ggaacgcggt agctggtgtg aactggtcgt tctgaccatc
     6301 aacgaaaaag cccacatctg tgggctttca tgtcaccaag agccgcggct cctttgcgta
     6361 tccttttatg tctcctcacc gtctggttgg tgtcctgctg agacttctaa cttcctgttt
     6421 ttgttggtgt tgtttttaca ccgtccaatc atgattggtg ggctggcgga gtctgaattg
     6481 gttgtgtaat tcattgaatt taatttggaa aatcctagtt caacttttcg gtggtgccta
     6541 tttaggtgcc taaaagtttt ttcaccctat tttggttagt aaaaggaaga gacataggga
     6601 aggagattga cgctacttaa gtgctcatta tccccgtagc tcgccttgct ctgagaatat
     6661 cggtagcgct gaggaatgac gtttgatcct gccatgaaaa tcccttccgg tcgatacgaa
     6721 ccagttcgta tttttcgacc aggaaattca ccgcatcagc aagtgttatt cccgcctcga
     6781 tatgttcttc gatagcagcg tcgtcatgga atggcgtgtc gttaagcgtc agtccgtaat
     6841 ggtgctccag cagatacgtt agcagttgct gccagacctg cacgggagac agacgtgacg
     6901 aaaccggcac cgtggtcggt acagataaaa tgtgcatgag ttgagtcctg ataaaagaaa
     6961 agaagctgtt actggctcga tgtctgagga taaataacga tgtaaacgta gccatgagag
     7021 ccgagagtat ccgcctcgca ggtgagtccg ttatgatgca acgtgacgca atgctgatgg
     7081 cgtggattta gttcaccggt tgtcagcatc aattcaagct gtttcatcac tagcggaaat
     7141 gcttgatcca gacgtagcgc ctcctcctca ttaaacgcac cgctaatatc tgcacggtcc
     7201 gccagaaaat gcaggcgacg gccttcctgc acaagccttg caccgaagca cggcgtcatg
     7261 tcgcgcttca gtccccatga atttttcatt tttctactcc taaatatcag ttagttgtga
     7321 tgcacatcag gcgtgtatac gggccatggt tgtcacaacg acgttctgca taaaccgcca
     7381 gtacaccgga aatctccgcg atatcctgac cggtgtaatg actggcgctg ccgtgccact
     7441 ggtatttgcc tgtgcagtaa cgaaagatcc gggaatccgg atgctgtcgc tgaattgcca
     7501 tcgcggccct tttatcgatg attttcatgg ttgcctcata gccagccacg ttcagcaaaa
     7561 gaaagtacat caccgatacc aacaataaga tggtccagca cccgtacctc tactattgct
     7621 aacgctttca ccagatgttg agtgatgtgt tgatcctgtt tactaggttc tgtctctccg
     7681 gatggatggt tatgggcaag aatcacagcg gcagcgttat ggcgtagcgc cagtttcaca
     7741 acttcacgtg ggtggacttc agtatggtta atcgtgcctg taaatagcgt ctcgcattgc
     7801 agaagacggt tctgattatc aagatataaa acaataaaaa cttcgcgctc ctgcaaagca
     7861 agacgtagtt gtagccaggt tttcgtgaag gttgtggaat taaattgctc gccaggctgg
     7921 cgctgatatt tttccagtaa gcggagagcg cgacgaatgg tgcgtgcttc agttaccgga
     7981 agatcatgag gaagatttat ttctgacatg acgcggcctt agaggtaata gtaaaagttg
     8041 tttacgaagc aagttgaagc atattctcag ccatcaccca tagagcgcga ttaagcttta
     8101 cgtctccgtc gatacctttg accgcacggg tttgagcacg cctacctttc gtcgttcgtc
     8161 ccgatagccc gccttttatc aagttctcct gaatgcgttg gtaagtagtc cacaggtcat
     8221 tgctctcatc ctgccagcgg cgcggggaaa ggatctgcga ttccgtcacc ggttgatgct
     8281 cttcaccaaa gcgataggtt aatgccgctt tcgccatcgc atgctgtgct ggtggcggta
     8341 acaaaagcga ttgcatggca tcgcgctttt cttccacccg gtcgaatatc cccagcactt
     8401 cgtaagcccc ttcaatgact ttctctacca cattgccttt atgcggcaca cgtacttctc
     8461 caaaactctc accgcaaatc agtccgttct gacagactgc acgaaatagc cccggcaaca
     8521 tctggtatgc gctggaacca tcatggctgt tgagcagaat gatttccggc acctgcttac
     8581 cggtgatttg cccttcacgg cgcaggcgca gcatgtgttt cgtgtgctct cgcttgctct
     8641 ggtcgcgcac tcgagtctgg caggcaaaga atggctgaaa gccttcacgc tgcaggttat
     8701 caagcagggt aatggtcggg atgtaggtgt accgctcact gcgtgattca tgtttttctt
     8761 cgctgaacac actcgggaca tagtgcgcta gttcatctcg ggttaatgga cgatcacggc
     8821 gaacgagatt gaccgcacca aagcggcttg ctaatcgcat ggaataactc cttattaata
     8881 aaaataaatc ccttgttcat gattgagcaa gagttgaatt tttttaatga taaaataacg
     8941 ttgtattcgg ttgggtaata taggtgtgat ttaaattcta tctgattttt ttattatcca
     9001 ttatatgtga taactccaga ctaaaattta atgttttggg attttgattt tccagaaata
     9061 atagttttct tcttttattg ccatctttgc gatttgtgga tag
//
LOCUS       DAZFFG010000158         8765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFFG010000158 DAZFFG010000000
VERSION     DAZFFG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.159"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..181)
                     /locus_tag="ACHJ7J_004425"
     CDS             complement(<1..181)
                     /locus_tag="ACHJ7J_004425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002565.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPG"
     gene            complement(341..553)
                     /gene="rpmE"
                     /locus_tag="ACHJ7J_004426"
     CDS             complement(341..553)
                     /gene="rpmE"
                     /locus_tag="ACHJ7J_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO5002566.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            756..2954
                     /gene="priA"
                     /locus_tag="ACHJ7J_004427"
     CDS             756..2954
                     /gene="priA"
                     /locus_tag="ACHJ7J_004427"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO5002567.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALISRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3110..4135
                     /gene="cytR"
                     /locus_tag="ACHJ7J_004428"
     CDS             3110..4135
                     /gene="cytR"
                     /locus_tag="ACHJ7J_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO5002568.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4227..5186
                     /gene="ftsN"
                     /locus_tag="ACHJ7J_004429"
     CDS             4227..5186
                     /gene="ftsN"
                     /locus_tag="ACHJ7J_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO5002569.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5279..5809
                     /gene="hslV"
                     /locus_tag="ACHJ7J_004430"
     CDS             5279..5809
                     /gene="hslV"
                     /locus_tag="ACHJ7J_004430"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO5002570.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5819..7150
                     /gene="hslU"
                     /locus_tag="ACHJ7J_004431"
     CDS             5819..7150
                     /gene="hslU"
                     /locus_tag="ACHJ7J_004431"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO5002571.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7217..8143
                     /gene="menA"
                     /locus_tag="ACHJ7J_004432"
     CDS             7217..8143
                     /gene="menA"
                     /locus_tag="ACHJ7J_004432"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO5002572.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8236..8721
                     /gene="rraA"
                     /locus_tag="ACHJ7J_004433"
     CDS             8236..8721
                     /gene="rraA"
                     /locus_tag="ACHJ7J_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO5002573.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2129 a         2279 c         2399 g         1958 t
ORIGIN      
        1 caccgggaag gacctttgca cccagcagcg gattgaccgg atggccggac gtcaccccgc
       61 cggggcacac cgaacaggcc acgccggtgg gggcaccgat gcgcaccccg gctgaaccct
      121 ggacaatgct accgccatac tgcgtcatgt cgccctgacg cgccgccggt tttccgctca
      181 tcgtaaaatc cttttgaaat aaataagaac cagaagccta gcgccagtca acaacagaaa
      241 atgataatga gttaacaccc cggaattttt cagaatttat aaaaggcaaa ttacaggcac
      301 aaaaaaagcg ccgtgcggcg cttttttcgg aaatccggtc ttatttgctg cccgggatgt
      361 tgaaacgctt gttgaagcgg tcaacacggc caccggtagc aacatcacgc tgtttgccag
      421 tgaagaacgg gtggcacttg ctgcacacgt cgaggttcag gtcatgacca acggtggagc
      481 ggattttcat tacgttaccg caagagcagc tagcagtaat ttcttcgtat ttcgggtgaa
      541 tatctttttt catgggaaaa cctcggttta aggccgcgtc gctcttccag ccctaacgcc
      601 agacaccacg cgatgttaaa agtatagctt caatacgatc atttcgtacg aaggcgcgaa
      661 atcatacaga aattaaccag cgtatgcaaa ctgatccgca ctcttctacg gcaatgtgta
      721 tactaaccca ccgaatttca agtcaggatg atgctatgcc cgttgcccac gttgccttgc
      781 ccgttccgct tcctcgtacc tttgactatc tgctgccaga aggcatgacg gttaaagctg
      841 ggtgtcgcgt gcgcgtgccg tttggcaaac agcaggagcg catcgggatt gtggtatcag
      901 ttagcgatgc cagcgaactg ccgctcaatg agctaaaagc ggtagtcgaa gtgctggata
      961 gtgagccggt gtttactcac tccgtctggc gattgctgct ctgggcggcg gattactatc
     1021 atcatccgat tggcgatgtg ctgtttcatg ccttgccgat tttactacgc caggggcggc
     1081 ctgcggcgaa cgcgccgatg tggtactggt ttgccactga acaaggccat gcggtggatc
     1141 tgaacagcct gaaacgttcg cccaagcaac aacaggcgct ggcagcgtta cggcaaggca
     1201 aaatctggcg tgatcaggtc gcggaacttg aatttaatga tgccgcgttg caggcgctgc
     1261 gcaaaaaagg tctgtgtgat ttagcaagtg aaacaccaga gtttagcgac tggcgaacga
     1321 actatgccgt ttctggtgaa cggttgcgat tgaataccga acaggccacc gccgttggcg
     1381 caattcatag cgcggcggat accttctctg cctggctgct ggcgggcgtt acaggttccg
     1441 gtaaaacaga ggtttatttg agcgtactgg aaaacgtgct cgctcagggc aaacaggcgc
     1501 tggtgatggt gccggaaatc ggcctgacac cgcaaactat cgcccgtttt cgtgaacgtt
     1561 ttaatgcccc cgtagaagtt ctgcattccg gcctgaacga cagcgagcgt ctttcggcgt
     1621 ggttgaaagc gaaaaatggt gaggcggcga ttgtgatcgg cacccgctcc gcgctgttta
     1681 cgccgtttaa aaatctcggt gtgattgtca ttgatgaaga gcacgacagt tcctacaagc
     1741 agcaggaagg ctggcgctat catgcccgcg acctggcggt ttatcgtgcg cacagcgagc
     1801 aaatcccgat tattcttggc tccgcaacgc ccgcgctgga aacgttatgc aacgtccagc
     1861 agaaaaaata ccgcctgctg cgcctgaccc gtcgggcagg gaatgcgcgt ccagcaattc
     1921 aacatgtgct ggatttaaaa ggccagaagg tgcaggcggg gctggctccg gcgttaatca
     1981 gccgtatgcg ccagcattta caggccaata atcaggttat cctctttctt aatcgtcggg
     2041 gttttgcgcc tgcgctgctg tgccacgact gcggctggat agccgaatgc ccacgttgcg
     2101 atcactacta cacgctgcat caggcgcaac agcatttgcg ctgtcaccac tgtgacagcc
     2161 aacgcccggt gccgcgccag tgcccttcct gcggttccac gcacctggta cccgtggggc
     2221 tgggcaccga acagcttgaa cagacgctcg cgccgttatt ccctggtgtg cccatttctc
     2281 gtatcgaccg cgataccacc agccgcaaag gagcgctgga acaacaactg gcggaagtgc
     2341 atcgcggcgg cgcgaggatc ttgattggca cgcaaatgct ggcgaaaggt caccactttc
     2401 cggatgtgac gctggtagcg ttactggatg tggacggcgc gctgttttct gccgattttc
     2461 gctcggcaga gcgttttgcc cagctataca cccaggtagc aggccgtgcc gggcgtgcgg
     2521 gtaaacaggg cgaagtggtg ctgcaaacgc atcatccgga acacccgcta ttgcaaacgt
     2581 tgctctataa aggctacgac gcttttgccg aacaggcact ggctgagcga cgcatgatgc
     2641 agctgccgcc gtggaccagc catgtgattg tgcgtgcgga agatcataac aatcagcatg
     2701 cgccgctgtt cctgcaacaa ctgcgtaatc tgatcctctc cagcccgctg gcagacgaca
     2761 aattgtgggt tctcggtccg gttccggcac tggcacctaa acgtggcggt cgctggcgct
     2821 ggcagatatt gttgcagcac ccttcccgcg tgcgcttgca acacatcatt aacggtacgc
     2881 tggcactcat caatacaata ccggattccc gtaaggtgaa atgggtgctg gatgttgatc
     2941 cgattgaggg ttaaaccgct cacgatgcga ggcggatcga aaaattcaat attcatcaca
     3001 cttttcatga aaattctgta accgttttca cgcgctatct gctaaaaatg ttgccgatgt
     3061 gaagtaaaca tggatgtagt acgcctgacg tgccaggcga ggagtgagtg tgaaagcgaa
     3121 gaagcaggaa actgccgcga ccatgaaaga cgttgccctc aaggcaaaag tctctacagc
     3181 gactgtctcc cgagcattaa tgaatcccga taaagtctcc caggccaccc gtaatcgggt
     3241 tgaaaaagcg gcccgggaag tgggttattt accgcagcct atggggcgca acgtcaagcg
     3301 taatgaatcc cgcaccattc tggtgattgt cccggatatc tgcgatccct tctttagcga
     3361 aattattcgc ggtatagaag ttacggcggc aaatcacgga tatctggtgc tgattggcga
     3421 ctgtgcgcat caaaatcagc aggaaaaaac ctttatcgat ttgatcatca ccaagcaaat
     3481 tgatggcatg ttgctgctgg gttcaaggct gccgtttgat gccagcattg aggaacagcg
     3541 taatctgccg ccgatggtga tggcgaacga atttgcaccg gaactggagc tgcctaccgt
     3601 tcatatcgac aatctgaccg ccgcatttga tgcagtaaat tatttatatg agcaagggca
     3661 taaacggatt ggctgtatag ccggtcccga agagatgccg ctgtgtcact accgcctgca
     3721 aggttacgtt caggcgctgc gtcgctgcgg cattatggtt gatccgcaat acatcgcccg
     3781 tggcgacttc accttcgaag ccggaagcaa agcaatgcaa cagctgcttg atcttccaca
     3841 accgcctact gctgtcttct gccatagcga tgtaatggca ctcggcgcac tttcgcaggc
     3901 aaaacgccag gggctgaaag taccggaaga cctttccata atcggttttg ataacatcga
     3961 cctgacgcaa ttttgtgatc cgccgctgac aaccatcgcg cagccacgtt acgaaatcgg
     4021 tcgggaagct atgctgttat tgcttgatca aatgcagggg caacacgttg gcagtggctc
     4081 tcgtttaatg gactgcgaac ttatcatccg gggatcaaca cgcgcgttac cttaaagtaa
     4141 acctttaaga ctgccgtgac tggtcaaagg cccgccgctt aagtaacatg gcgggctgac
     4201 gaacgaataa atacagcgaa acgatagtgg cacaacgaga ttatgtacgc cgcagccaac
     4261 cggcaccttc gcggcgaaaa aagagcacct cacggaaaaa gcaacgaaat ctgcctgcgg
     4321 tttctcccgc tatggtcgct attgctgccg ccgttcttgt gacctttatc ggtggtctgt
     4381 acttcattac gcatcacaag aaagaagagt ccgagacgct gcaaagccag aaagtgaccg
     4441 gaaacggact accaccaaaa ccagaagaac gctggcgcta catcaaagag ctggaaagtc
     4501 gccagccggg tgtgcgtgcg cccacagaac cttctgccgg tggtgaagtg aaaacgccgg
     4561 agcaactgac accagaacaa cgtcagcttc ttgaacaaat gcaggctgat atgcgccagc
     4621 agccaacgca gctggttgag gttccgtgga atgaacagac gccggaacag cgtcagcaaa
     4681 cgctacagcg ccaacgtcag gcgcagcaat tagcggaaca gcaacgcctg gctcagcagt
     4741 cgcgtacgac tgaacaaagc tggcagcagc agacgcgtac gtcgcaagcc gcgccggtac
     4801 aggcgcaacc gcgccagtct aaaccggctt ctactcagca gccgtaccag gatctgctgc
     4861 aaacccctgc gcacacgacc gcgcagtcga agccgcagca agctgcgccg gttactcgtg
     4921 ccgctgacgc gccaaaaccg acggcggaga aaaaagacga acgccgctgg atggtgcagt
     4981 gcggttcgtt cagaggcgcg gaacaggcag agacggtacg tgctcagctg gcgttcgaag
     5041 gctttgactc aaaaattacc accaacaatg gctggaatcg tgtggtcatt ggcccggtga
     5101 aaggcaaaga gaacgcagac agcaccctca atcggttgaa gatggcgggt catacaaact
     5161 gcattcggct cgccgccggg ggttgaaacc ctcaaaatcc cccccatcta taattgcatt
     5221 atgccccgta cttttgtacg gggtttgtac tctgtattcg taaccaaggg gtcagctcgt
     5281 gacaactata gtaagcgtac gccgtaacgg ccatgtggtc atcgctggtg atggtcaggc
     5341 cacgttgggc aataccgtaa tgaaaggcaa cgtgaaaaag gtccgccgtc tgtacaacga
     5401 caaagtcatc gcgggctttg cgggcggtac tgcggatgct tttacgctgt tcgaactgtt
     5461 tgaacgtaaa ctggaaatgc atcagggcca tctggttaaa gccgccgttg agctggcaaa
     5521 agactggcgt accgatcgca tgctgcgcaa acttgaagca ctgctggcag tcgcggatga
     5581 aactgcatcg cttatcatca ccggtaacgg tgacgtggtg cagccagaaa acgatcttat
     5641 tgctatcggc tccggcggcc cttacgccca ggctgcggcg cgtgcactgt tagaaaatac
     5701 tgaacttagt gctcgtgaaa ttgctgaaaa ggcgttggat attgcaggcg acatttgcat
     5761 ctataccaac catttccaca ccatcgaaga attaagctac aaagcgtaag gatctcccat
     5821 gtctgaaatg accccacgcg aaatcgtcag cgaactggat aagcacatca tcggccagga
     5881 caacgccaag cgttctgtgg cgattgctct gcgtaaccgc tggcgtcgca tgcagctcaa
     5941 cgaagagctg cgccatgaag tgaccccgaa aaatatcctg atgatcggcc cgaccggtgt
     6001 cggtaaaact gaaatcgccc gtcgtctggc taagttggcg aatgcgccgt tcatcaaagt
     6061 tgaagcgacc aaattcaccg aagtgggcta cgtcggtaag gaagtggatt ctattattcg
     6121 cgatctgacc gatgccgccg tgaaaatggt acgcgtccag gctatcgaga aaaaccgtta
     6181 tcgcgctgaa gaactggcag aagaacgtat tctcgacgtg ctgatcccac ctgctaaaaa
     6241 caactgggga cagaccgaac agcagcagga accgtccgct gctcgtcagg cattccgcaa
     6301 aaaactgcgt gaaggccagc ttgatgacaa agaaatcgag atcgatcttg ccgcagcacc
     6361 gatgggcgtt gaaattatgg ctcctccagg tatggaagag atgaccagcc agctgcagtc
     6421 catgttccag aacctgggcg gccagaagca aaaagcgcgt aagctgaaaa tcaaagacgc
     6481 catgaagctg ctgattgaag aagaagcggc gaaactggtg aacccggaag agctgaagca
     6541 agacgctatc gacgctgttg agcagcacgg gatcgtgttt atcgacgaaa tcgacaaaat
     6601 ctgtaagcgc ggcgagtctt ccggtccgga tgtttctcgt gaaggcgttc agcgtgacct
     6661 gctgccgctg gtagaaggtt gcaccgtttc caccaaacac gggatggtca aaactgacca
     6721 cattctgttt atcgcttctg gcgcgttcca gattgcgaaa ccgtctgacc tgatcccgga
     6781 actgcaaggt cgactgccaa tccgcgttga actgcaggca ctgaccacca gcgactttga
     6841 gcgtattctg accgagccga atgcctctat caccgtgcag tacaaagcac tgatggcgac
     6901 tgaaggcgta aatatcgagt ttaccgactc cggtattaaa cgcatcgcgg aagcggcatg
     6961 gcaggtgaac gaatctaccg aaaacatcgg tgctcgtcgt ttacacactg ttctggagcg
     7021 tttaatggaa gagatttcct acgacgccag cgatttaagc ggtcaaaata tcactattga
     7081 cgcagattat gtgagcaaac atctggatgc gttggtggca gatgaagatc tgagccgttt
     7141 tatcctataa tcgcgttcaa tcattttcat cattgtttga tggggctgaa aggccccatt
     7201 tttattggcg cgtattatga ctgaacaaca aattagccga actcaggcgt ggctggaaag
     7261 tttacgacct aaaaccctcc ccctcgcctt tgctgcaatt atcgtcggga cggcgctggc
     7321 atggtggcaa ggtcattttg atccgctggt cgccctgctg gcactgatta ccgccgggct
     7381 attacagatc ctttctaacc tcgccaatga ttacggcgat gcagtaaaag gcagcgataa
     7441 acctgaccgc attgggccgc tacgcggcat gcaaaaaggg gtcattaccc agcaagagat
     7501 gaaacgggcg ctcattatta ccgtcgtgct catctgtctc tctgggctgg cactggttgc
     7561 agtggcatgc catacgctgg ctgattttgt cggtttcctg attcttggcg ggttgtcgat
     7621 tattgccgct atcacctaca ccgtgggcaa tcgtccttat ggttatatcg gtctgggtga
     7681 tatttccgta ctggttttct ttggctggtt aagcgtcatg gggagctggt atttacaggc
     7741 tcatacattg attccggcac tgatccttcc ggcgaccgca tgcggcctgc tggcaacggc
     7801 agtactgaac attaataacc tgcgtgatat caatagcgac cgcgaaaacg gcaaaaacac
     7861 gctggtggtg cgcttaggtg aagtgaacgc gcgtcgttat catgcctgcc tgctgatggg
     7921 ctctctggta tgtctggcgc tgtttaatct cttttcgctg catagcctgt ggggctggct
     7981 gttcctgctg gcggcaccgt tgctggtgaa gcaagcccgt tatgtgatgc gggaaatgga
     8041 cccggtggcg atgcgaccga tgctggaacg cactgtcaag ggagcgttac tgactaacct
     8101 gctgtttgtt gtagggatat tcctaagcca gtgggcagca taactgacaa atatcaatta
     8161 acaattgatg attttgccaa cagaccacat agcgcgatat actgaaaatt ctcgcagcaa
     8221 ctgaatgtta agcctatgaa atacgatact tccgagcttt gtgacatcta tcaagaagat
     8281 gttaacgtcg tggaaccgct gttctccaac tttggcggac gggcgtcgtt tggcggacaa
     8341 ataatcacgg taaaatgttt cgaggacaac gggttgctgt acgatctgct cgaacagaat
     8401 ggccgtggtc gtgttcttgt cgtcgatggc ggtggttctg ttcgtcgcgc actggtcgat
     8461 gctgaactgg cgcgtctggc agtacaaaat gaatgggaag gtctggtcat ttacggcgcg
     8521 gtgcgtcagg tagatgacct ggaagagttg gatatcggca tccaggcgat ggcggcaatt
     8581 ccggttggtg ccgctggcga aggcattggc gaaagcgatg tccgcgtcaa ttttggcggt
     8641 gttaccttct tctccggcga ccatctttat gccgacaata ccgggattat tctttcagaa
     8701 gatccgctgg atattgaata agtatctgcc atgcgttaaa aggggaatta aattcccctt
     8761 tttta
//
LOCUS       DAZFFG010000159         8734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFFG010000159 DAZFFG010000000
VERSION     DAZFFG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.160"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(365..628)
                     /gene="rpsT"
                     /locus_tag="ACHJ7J_004434"
     CDS             complement(365..628)
                     /gene="rpsT"
                     /locus_tag="ACHJ7J_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO5002574.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            731..949
                     /locus_tag="ACHJ7J_004435"
     CDS             731..949
                     /locus_tag="ACHJ7J_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO5002575.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            957..1898
                     /gene="ribF"
                     /locus_tag="ACHJ7J_004436"
     CDS             957..1898
                     /gene="ribF"
                     /locus_tag="ACHJ7J_004436"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO5002576.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1941..4757
                     /gene="ileS"
                     /locus_tag="ACHJ7J_004437"
     CDS             1941..4757
                     /gene="ileS"
                     /locus_tag="ACHJ7J_004437"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO5002577.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4757..5251
                     /gene="lspA"
                     /locus_tag="ACHJ7J_004438"
     CDS             4757..5251
                     /gene="lspA"
                     /locus_tag="ACHJ7J_004438"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO5002578.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5339..5788
                     /gene="fkpB"
                     /locus_tag="ACHJ7J_004439"
     CDS             5339..5788
                     /gene="fkpB"
                     /locus_tag="ACHJ7J_004439"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5002579.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5790..6740
                     /gene="ispH"
                     /locus_tag="ACHJ7J_004440"
                     /gene_synonym="lytB"
     CDS             5790..6740
                     /gene="ispH"
                     /locus_tag="ACHJ7J_004440"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO5002580.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6806..7720
                     /gene="rihC"
                     /locus_tag="ACHJ7J_004441"
     CDS             6806..7720
                     /gene="rihC"
                     /locus_tag="ACHJ7J_004441"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO5002581.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7887..8708
                     /gene="dapB"
                     /locus_tag="ACHJ7J_004442"
     CDS             7887..8708
                     /gene="dapB"
                     /locus_tag="ACHJ7J_004442"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO5002582.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLHNL"
BASE COUNT         2002 a         2154 c         2476 g         2102 t
ORIGIN      
        1 gattgctgtt aataatttac aaggtctaat gaggagatat ttatgaatgg ttaaagtata
       61 taggaattta attcaaaaat agagtggtct taagtttaat acctcaatgt gtatcacaat
      121 atccatattc tttgtggggg agtctggtga ttgagtagat attcttgttc agaatgtatc
      181 agccgatggt tctacgattc ttaagccacg aagagttcag atagtgcaac ggcatgtctg
      241 caagaacctg caacttttga ctatctggca accggcagtg tgttctctca cgcatcacaa
      301 aagcagcagg cataaaaaaa cccgcttgcg cgggcttttt cacaaagctt cagcaaattg
      361 gcgattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt
      421 ttttgtggat cagaccttta gcagcctgac ggtccacgat cggttgcatt tcgttaaatg
      481 ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag
      541 tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc
      601 gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca
      661 attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata
      721 agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc
      781 ggggtgtgaa tacagctttt ccgcgataaa aattgcagca ggcggtcagt ttcttcccgt
      841 gatttgcgcc atggcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
      901 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
      961 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
     1021 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
     1081 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
     1141 tgtttgctac cgataaagcc ccggcaagac tgacccggct gcgggaaaaa ctgcgttacc
     1201 ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt tttgcggcgt
     1261 taaccgcgca aaatttcatc agcgatcttt tggtgaagca tttgcgcgta aaatttcttg
     1321 ccgtaggtga tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga
     1381 aagctggcat ggaatacggc ttcgatatca ccagtacgca aactttttgc gaaggtggtg
     1441 tgcgtatcag cagcaccgcc gtgcgtcagg cgctggctga tgacaatctg gctctggcag
     1501 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
     1561 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
     1621 aaggggttta tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa
     1681 acatcggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag
     1741 atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
     1801 atgagcagcg atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
     1861 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
     1921 aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
     1981 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
     2041 atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc
     2101 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
     2161 tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
     2221 gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg
     2281 gtgagaaatt caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg
     2341 ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
     2401 acctgaccat ggacttcaaa actgaagcca atatcatccg cgcgctgggc aaaatcatcg
     2461 gcaatggtca cctgcacaaa ggcgcgaagc cggtgcactg gtgcgtagac tgccgttctg
     2521 cactggcaga agcggaagtt gagtattacg acaaaacttc tccgtccatt gacgtcgctt
     2581 tccaggcggt cgatcaggat gcgctgaaag cgaaatttgg cgtaagcaac gttaacggcc
     2641 caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct
     2701 ctattgcacc tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg
     2761 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg
     2821 gcacggtaaa aggtgcagag cttgagctgc tgcgctttac ccatccgttt atgggcttcg
     2881 acgttccggc aatcctcggc gatcacgtta ccctggatgc cggtaccggt gccgttcaca
     2941 ccgcgcctgg ccacggcccg gacgactatg taatcggtca gaaatatggt ctggaaaccg
     3001 ctaacccggt tggcccggac ggtacttatc tgccgggcac ttacccgacg ctggatggcg
     3061 tgaacgtctt caaggcgaac gatattgtcg ttgcgctgct acaggaaaaa ggcgcgctgc
     3121 tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca
     3181 tcttccgcgc gacaccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt
     3241 cactgaaaga gatcaaaggc gtgcagtgga tcccggactg gggccaggcg cgtatcgagt
     3301 cgatggttgc taaccgtcct gactggtgta tctcccgtca gcgcacctgg ggcgtaccga
     3361 tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtaccctc gaactgatgg
     3421 aagaagtggc taaacgcgtt gaagttgacg gcatccaggc gtggtgggat ctcgatgcga
     3481 aagagatcct cggcgacgaa gctgaccagt atgtgaaagt gccggatacg ctggatgtat
     3541 ggtttgactc cggatctacc cactcttctg ttgttgatgt gcgtccggaa tttgccggtc
     3601 acgcagcgga catgtatctg gaaggttcag accagcaccg tggttggttc atgtcttctt
     3661 tgatgatctc caccgcgatg aagggcaaag cgccgtatcg tcaggtactg acccacggct
     3721 ttaccgtgga tggtcagggc cgcaagatgt ctaaatctat cggcaacacc gtttcgccgc
     3781 aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaactgact
     3841 acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgcagccgac agctatcgtc
     3901 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccggcaaaag
     3961 atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag
     4021 cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc
     4081 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
     4141 gtcagtacac cgccaaagcg gatagcgtgg cgcgtcgtag ctgccagact gcgctgtatc
     4201 acatcgctga agcgctggtg cgctggatgg caccaatcct ctccttcacc gctgatgaag
     4261 tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggtgag tggtacgaag
     4321 gcctgtttgg tctggcagac agtgaagcaa tgaacgatgc gttctgggac gagctgttga
     4381 aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg
     4441 gctcgctgga agcggcagta accttgtatg cagaaccgga actggcggcg aaactgaccg
     4501 cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata
     4561 acgacgcacc tgctgatgct cagcagagcg aagtactcaa agggctgaaa gtcgcgttga
     4621 gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg
     4681 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
     4741 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
     4801 ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
     4861 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
     4921 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
     4981 tgcgattggt attagcgtga tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa
     5041 gctaaacaat atcgcttatg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
     5101 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
     5161 caccttcaac cttgccgata ctgccatttg tgtcggtgcg gcactgattg tgctggaagg
     5221 ttttttgcct tctaaagcga aaaaacaata ataaaccctg ctggataagg cctacagatt
     5281 gctgcgaaat cgtaggccgc atccggcaaa aatcctcaaa atataagagc aaacctgcat
     5341 gtctgaatct gtacagagca atagcgccgt cctggtgcac ttcacgctaa aactcgacga
     5401 tggcaccacc gccgagtcta cccgcaacaa cggtaaaccg gcgctgttcc gcctgggcga
     5461 tgcttctctt tctgaagggc tggagcaaca cctgctgggg ctgaaagtgg gcgataaaac
     5521 caccttctcg ctggagcccg atgcggcgtt tggcgtgccg tcaccggacc tgattcagta
     5581 cttctcccgc cgtgaattta tggatgcagg cgagccagaa attggcgcaa ttatgctttt
     5641 taccgcaatg gatggcagtg agatgcctgg cgtgatccgc gaaattaacg gcgactccat
     5701 taccgttgat ttcaaccatc cgctggccgg gcagaccgtt cattttgata ttgaagtgct
     5761 ggaaatcgat ccggcactgg aggcgtaaca tgcagatcct gttggccaac ccgcgtggtt
     5821 tttgtgccgg ggtagaccgc gctatcagca ttgttgaaaa cgcgctggcc atttacggcg
     5881 caccgatata tgtccgtcac gaagtggtac ataaccgcta tgtggtcgat agcttgcgtg
     5941 agcgtggggc tatctttatt gagcagatta gcgaagtacc ggacggcgcg atcctgattt
     6001 tctccgcaca cggtgtttct caggcggtac gtaacgaagc aaaaagccgc gatttgacgg
     6061 tgtttgatgc cacctgtccg ctggtgacca aagtgcatat ggaagtcgcc cgcgccagcc
     6121 gtcgtggcga agagtcgatt ctcatcggcc acgccgggca ccctgaagtg gaagggacaa
     6181 tgggtcagta cagcaacccg gaagggggaa tgtatctggt tgaatcgcca gacgatgtgt
     6241 ggaaactgac ggtcaaaaac gaagagaagc tctcctttat gacccaaacc acgctgtcgg
     6301 tggatgacac gtctgatgtg atcgacgcgc tgcgtaaacg cttcccgaaa attgtcggtc
     6361 cgcgcaaaga tgacatctgc tacgccacga ctaaccgtca ggaagcggta cgcgccctgg
     6421 cagaacaggc ggaagttgtg ttggtggtcg gttcgaaaaa ctcctccaac tccaaccgtc
     6481 tggcggagct ggcccagcgt atgggcaaac gcgcgttttt gattgacgat gcgaaagata
     6541 tccaggaaga gtgggtgaaa gaggttaaat gcgtcggcgt gactgcgggc gcatcggctc
     6601 cggatattct ggtgcagaat gttgtggcac gtttgcaaca gctgggcggt ggtgaagcca
     6661 ttccgctgga aggccgtgaa gaaaacattg ttttcgaagt gccgaaagag ctgcgtgtcg
     6721 atattcgtga agtcgattaa gtcattagca gcctaagtta tgcgaaaatg ccggtcttgt
     6781 taccggcatt ttttatggag aaaacatgcg tttacctatc ttcctcgata ctgaccccgg
     6841 cattgacgat gccgtcgcca ttgccgccgc gatttttgcc cccgaactcg acctgcaact
     6901 gatgaccacc gtcgcgggta atgtctcggt tgagaaaact acccgtaacg ccttgcaact
     6961 gctgcatttc tggaatgcgg agattccgct cgcccaggga gccgctgtgc cgctggtacg
     7021 cgcaccgcgt gatgcagcat ctgtgcacgg cgaatcggga atggctggct acgactttgt
     7081 tgagcacaac cgaaagccac tcgggatacc agcgtttctg gcgattcgtg atgccctgat
     7141 gcgtgcacca gagcccgtta ccctggtggc catcggcccg ttaaccaata ttgcgctgtt
     7201 actttcacaa tgcccggaat gtaaaccgta tattcgtcgt ctggtgatca tgggcggttc
     7261 tgctggtcgc ggcaactgta caccaaatgc cgagtttaat atcgctgccg atccagaagc
     7321 ggctgcctgt gtcttccgta gcggcatcga aattgtcatg tgcggtctgg atgtcaccaa
     7381 tcaggcaata ttaacccctg actatctcgc tactttgccg gagttgaatc gcaccggaaa
     7441 aatgctccac gccctgttta gccactaccg tagcggcagt atgcaaagcg gtctgcgaat
     7501 gcacgatctc tgcgccatcg cctggctggt gcgcccggac ttattcaccc tcaagccgtg
     7561 ttttgtcgcg gtagaaactc aaggcgaatt tacctcaggc acgacggtgg ttgatatcga
     7621 cggttgcctg ggcaaaccag ctaatgtaca ggtggcattg gatctgaatg taaaaggctt
     7681 ccaacagtgg gtggctgagg tgctggctct ggtgccgtaa cctgtcacat gttatcggca
     7741 tgcagttatt catcgactca tgcctttcac tgatatccct ccctgtttat cattaatttc
     7801 taattatcag cgtttttggc tggcggcgta gcgatgcgct ggttactctg aaaacggtct
     7861 atgcaaatta acaaaagaga atagctatgc atgatgcaaa catccgcgtt gccatcgcgg
     7921 gagccggggg gcgtatgggc cgccagttga ttcaggcggc gctggcatta gagggcgtgc
     7981 agcttggcgc ggcgctggag cgtgaaggat cttctttact gggcagcgat gccggtgagc
     8041 tggctggtgc aggaaaaaca ggcgttactg tgcaaagcag tcttgatgcg ataaaagatg
     8101 attttgatgt gtttatcgat tttacccgtc cggaaggtac gctgaaccat ctcgcttttt
     8161 gtcgccagca tggcaaaggg atggtgatcg gcactacggg gtttgacgaa gccggtaaac
     8221 aggcgattcg tgacgccgcc gccgatattg cgattgtctt tgccgccaat tttagcgttg
     8281 gcgttaacgt catgcttaag ctgctggaga aagcagccaa agtgatgggt gactacaccg
     8341 atatcgaaat tattgaagca catcatagac ataaagttga tgcgccgtca ggcaccgcac
     8401 tggcaatggg agaggcgatc gcccacgccc ttgataaaga tctgaaagat tgcgcggtct
     8461 acagtcgtga aggccatacc ggtgaacgtg tgcctggtac cattggtttt gccaccgtgc
     8521 gtgcaggtga catcgttggc gaacataccg cgatgtttgc cgatattggc gagcgtctgg
     8581 agatcaccca taaggcgtcc agccgcatga catttgctaa cggtgcggta agatcggctt
     8641 tgtggttgag tggtaaggaa agtggtcttt ttgatatgcg agatgtgctt gatctccata
     8701 atttgtaacc acaaagtgtt tatggtgatg caaa
//
LOCUS       DAZFFG010000160         8580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFFG010000160 DAZFFG010000000
VERSION     DAZFFG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.161"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..735
                     /locus_tag="ACHJ7J_004443"
     CDS             <1..735
                     /locus_tag="ACHJ7J_004443"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5002583.1"
                     /translation="ALEKLIVKKQAIKTATMQRLLTGKTRLPQFALRKDGSAKGYKKS
                     ELGEIPEDWNCINLGELGNCIIGLTYKPEDTSDYGTLVLRSSNIQNNHLAYDDNVFVS
                     IEPPERTIVKEGDILVCVRNGSRQLIGKCALIDKNAQGSAFGAFMSVFRTDIYGFTFH
                     QFQSDIIQKQIAEVMGATINQITNKDMLGFKIPLPENTEEQAAIAAILSDMDKEIQIL
                     QQRLDKTRQLKQGMMQELLTGKIRLI"
     gene            748..1404
                     /gene="darT"
                     /locus_tag="ACHJ7J_004444"
     CDS             748..1404
                     /gene="darT"
                     /locus_tag="ACHJ7J_004444"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system toxin DNA ADP-ribosyl
                     transferase DarT"
                     /protein_id="HFO5002584.1"
                     /translation="MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAEN
                     WINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTPFSPMLMNIHSGRGGIKQRPNEE
                     IVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLNSLDQIDWPILQARDFRRDPDD
                     PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS
                     "
     gene            1401..2468
                     /gene="darG"
                     /locus_tag="ACHJ7J_004445"
     CDS             1401..2468
                     /gene="darG"
                     /locus_tag="ACHJ7J_004445"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin DNA ADP-ribosyl
                     glycohydrolase DarG"
                     /protein_id="HFO5002585.1"
                     /translation="MITYTQGNLLDAPVEALVNTVNTIGVMGKGIALMFKERFPENMK
                     VYALACKQKQVITGKMFITETGELMGPRWIVNFPTKQHWRADSRMEWIEDGLQDLRRF
                     LIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
                     GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAH
                     YYGPYAPNLNHLLNALDGTYLKAEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWL
                     PALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEGLHQWPAGERWASRK
                     LRLFDNNNLQFAINRVMEFHC"
     gene            2487..5582
                     /locus_tag="ACHJ7J_004446"
     CDS             2487..5582
                     /locus_tag="ACHJ7J_004446"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015872650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO5002586.1"
                     /translation="MSTVGQRERATQQRLIQFFVEDLGYRHLSHRTGYTGNSNIDTNI
                     LSAWLKRRGVNDTLITRALRQLDSAAALGEGKKLYYANKEVYRLLRYGVKDKEGAGEQ
                     NQTVWLIDWANPQANDFAIADEVTVKGEYCKRPDLVLYVNGIALGVIELKRSSVHVSE
                     GIRQNLDNQKKTFIRDFFTTMQLVMAGNDTQGLRYGTIETPEKYYLEWKEDNPGDYTH
                     KLDFHLSRLCSKSRLLDIVHNYIAFDVGVKKLCRHNQFFGVEAAKQHIARREGGIIWH
                     TQGSGKSLTMVWLAKWIRENVPNSRVLIVTDRTELDEQIESVFMGVNEDIYRTSSGND
                     LIATLNHPNPWLICSLVHKFGRRSEAEDNAATDAFITELQQSLTKTFCAKGDLFVFVD
                     ECHRTQSGKLHNAMTAILPDALFIGFTGTPLMKKDKKKSVEVFGPYIHTYKFDEAVAD
                     GVVLDLRYEARDIDQYLTSEKKVDDWFEAKTRGLSNLARTQLKQKWGSMQKLLSSKSR
                     LEQIVNDILLDMDTRPRLMDGRGNAMLVCSSIYQACKVYEMFSQTELAGKVAIVTSYR
                     PDAASIKGEETGEGLTEKLSKHATYRKMLADYFEQSEEKAAARITEFEREVKQRFIEE
                     PGQMRLLIVVDKLLTGFDAPSATYLYIDKTMTDHTLFQAICRVNRLDGEDKEYGYIID
                     YKDLFRSLDKAITDYTTGAFDDYDRDDVAGLLKDRLEQAQLDLDSALEMVRSLCEPVK
                     TPRSLQDYQHYFCGKSGENQTSLNEKEALRLTFYQCVAHLLRAYASIANEMPEAGYTA
                     EEVESIRAEVAEFEKLRYEIKLTSGDLLDMKRFEPAMRHLLDMYVRADGSEVLMDFEE
                     LSLIELIVEKGLAATVALPDDIRNNQDAMAETIENNVRKTIVDENPVNPKYYGRMSQL
                     LDELITLRRQNALNYQQYLERIRDLCKQVIRPEQTAGACYPATMDTQAKRAFYDNFGH
                     DEVLATRIDTTIRYTKRAEWIGDRFKEREIANALREETASYNIDIDEVIALARQQKEY
                     H"
     gene            5582..6298
                     /locus_tag="ACHJ7J_004447"
     CDS             5582..6298
                     /locus_tag="ACHJ7J_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO5002587.1"
                     /translation="MPIMQIGSLHLLVNRKAIKNLHISVLPPDGKVRVSAPEHMTETA
                     IRMAVASRFRWIRKQQQDFSRQPRQSEREMVSGECHYLWGRRYRLLVIERHGRHEVKV
                     AGNNKLQLYVQPGTRDENKALALSEFYRHELKQQVAKLLPEWQDRIGVPLTFWGIKKM
                     KTFWGTCNTTTKRIWLNLELIKKPPECLEYILVHELVHLLERHHNARFRLHMDRFIPD
                     WRERRDLLNKMPLAFEAWEY"
     gene            complement(6526..6756)
                     /locus_tag="ACHJ7J_004448"
     CDS             complement(6526..6756)
                     /locus_tag="ACHJ7J_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002588.1"
                     /translation="MKLFIFSLFVIVTSMVSGIAIAELSYFILLIIKYLAYGEVDFRL
                     SEVLRGLRMGCVGGGILGFGIVLFRFLGIKGF"
     gene            complement(6753..7310)
                     /locus_tag="ACHJ7J_004449"
                     /pseudo
     CDS             complement(6753..7310)
                     /locus_tag="ACHJ7J_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7379..8116)
                     /locus_tag="ACHJ7J_004450"
     CDS             complement(7379..8116)
                     /locus_tag="ACHJ7J_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFO5002589.1"
                     /translation="MLLYGYLIFFFPKELIWFFVDSYVVENNALSFGDGFESNAAAAT
                     SWNKYAVGNNLTPEQTQAGLDKIAKGDMPDSINITKVIVDGYQDGVMIAGAWYLGPAA
                     SAGKVLGGGLLGLAANSGYQFYDLNQPQNANKSWDYLGSATSFTTGMMAPGRGVLANT
                     GIAMGGAFFTDGPNTASLAGAGIGAGLGGAFGKYAPVGVDKVLNANNIPNFVYDAIGG
                     VGREFINGFTKEALNNPSEEKSGAKND"
     gene            complement(8052..8546)
                     /locus_tag="ACHJ7J_004451"
     CDS             complement(8052..8546)
                     /locus_tag="ACHJ7J_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO5002590.1"
                     /translation="MECIKCKMSEANKESGLCDACENEELSKINGLLYLPALGLILGV
                     IGSTIELYSFTITAFNYFNETGFLSYYAMGAFALLICGFIVSLYATWLFFRRKKGTRK
                     AMIAYYATGLIIALYLTLLPAILFDVQLDSDNIRLLVSGIFGVVIWIPYFLFSKRINM
                     VFCR"
BASE COUNT         2365 a         2108 c         2038 g         2069 t
ORIGIN      
        1 gctctagaaa agcttatcgt taaaaaacaa gccattaaaa ccgctactat gcagcgacta
       61 ctgacgggta aaacccgttt gccgcagttt gcgttgcgca aagatggttc tgctaaggga
      121 tataaaaaga gcgagttggg ggagattcct gaggattgga attgtattaa tcttggtgag
      181 ctaggtaatt gcattattgg gctaacttac aaacctgaag atacatctga ttatggtaca
      241 ttggtattac gctcatccaa cattcaaaat aatcacttag cttatgatga taacgtcttc
      301 gtcagcatag aaccacctga gcgcacaatt gtgaaagaag gtgatatctt ggtatgtgta
      361 agaaatggca gtcgccaact tattggcaaa tgcgcattaa ttgacaaaaa tgcgcagggc
      421 tcagcattcg gtgcatttat gtcagtattt cgaactgata tatatggatt tacatttcac
      481 caattccagt ctgatattat tcaaaagcaa atcgctgaag ttatgggtgc cactatcaat
      541 cagataacta acaaagatat gttgggcttt aaaatcccat tacctgaaaa tacagaagaa
      601 caagccgcca tcgccgccat cctctccgat atggacaagg aaatccagat cctgcaacag
      661 cgtctggaca aaacccgcca gcttaagcaa gggatgatgc aggaactgtt aaccgggaaa
      721 atccgcttaa tatagaaagg aaagcacatg gcttatgatt acagcgccag cctcaacccg
      781 caaaaagccc tgatctggcg tatcgtccat cgggacaata tcccgtggat tctggataac
      841 gggctgcact gcggcaacag tctggtgcag gcggaaaact ggatcaatat cggcaacccg
      901 gagctgatcg gcaagcgtgc cgggcatccg gtacctgtcg gaacgggcgg gacactgcat
      961 gactatgtgc cgttttattt tacccccttc tcgccaatgc tgatgaacat ccacagcggg
     1021 cgcggcggca ttaagcaacg ccccaatgag gagatcgtta ttctggtcag caacttgcgg
     1081 aacgtggcag cacacgacgt gcccttcgtg tttaccgaca gccatgctta ctataactgg
     1141 accaactact acaccagtct gaatagtctg gaccagatcg actggcccat tttgcaggca
     1201 cgggactttc gccgcgatcc agacgatccg gctaaatttg aacgctatca ggcagaggcg
     1261 ctaatctggc aacactgccc gatttctctg ctggatggga taatttgtta cagcgaagag
     1321 gtcaggttac aattagaaca atggctgttt cagcgtaatc taacaatgtc cgttcacaca
     1381 cgggctggat ggtacttctc atgatcacat atacacaagg caatttactg gacgcaccgg
     1441 tggaagctct ggtgaatacg gttaatacca tcggtgtcat ggggaaaggc atcgcgttga
     1501 tgtttaaaga gcgcttcccc gaaaatatga aagtctatgc cctcgcctgc aaacaaaagc
     1561 aggtgatcac tggcaaaatg ttcattaccg aaaccgggga actgatggga ccgcgctgga
     1621 ttgttaattt tccgaccaaa cagcactggc gcgctgattc ccgcatggag tggattgaag
     1681 atggcttgca ggacttacgc cgctttttga ttgaagagaa cgtgcagtcc atcgccattc
     1741 caccactggg cgcaggcaac ggtggtttga actggccgga cgttcgggcg cagattgaat
     1801 ctgcccttgg cgaccttcag gacgttgata tcctgattta ccaaccgact gaaaaatacc
     1861 agaacgttgc caaaagcact ggcgtaaaaa agctcactcc cgccagagcc gccatcgctg
     1921 aactggtgcg tcgctactgg gtattgggga tggagtgcag cctactggaa atccagaaac
     1981 tggcctggct gttacaacgc gccattgagc agcatcaaca ggacgatatc ctcaaactgc
     2041 ggtttgaagc tcactattat ggcccctatg cgcccaatct gaaccatctg cttaatgcgc
     2101 tggacggcac ctatcttaaa gccgagaaac gcatcccgga cagccaaccg ctggatgtga
     2161 tctggtttaa cgatcagaaa aaagaacacg ttaatgcgta tctgaacaac gaagccagag
     2221 agtggctacc tgcgctggag caggtcagcc agttaattga cggctttgaa tcgccgtttg
     2281 gtctggagtt gctggcaacc gtagactggt tgctgagccg tggcgaatgt caaccaacgc
     2341 tggactccgt taaggaaggg ctgcaccagt ggcctgcggg cgaacgctgg gccagccgta
     2401 agttgaggct gtttgataat aataatctgc aatttgccat taaccgcgta atggagttcc
     2461 attgctgagg gtttaaggag cgccttttga gtaccgtggg ccagcgcgaa cgcgccacac
     2521 agcagcgtct gattcagttt tttgttgaag atttaggtta ccgccatctg agtcaccgca
     2581 ccgggtacac cgggaacagc aacatcgaca ctaatatact gagcgcgtgg ctaaaacggc
     2641 gcggcgttaa tgatacgttg atcacccggg cgttgcggca actggattcc gccgccgctc
     2701 tgggcgaagg caaaaaactc tattacgcca ataaagaggt ctatcgcctg ctgcgttacg
     2761 gcgtgaaaga caaagaaggc gcaggcgagc agaaccaaac cgtgtggctg attgactggg
     2821 ccaacccaca agccaatgat ttcgccatcg ccgatgaggt gacggtcaaa ggcgagtact
     2881 gcaagcgccc ggatctggtg ctgtacgtta acggcattgc gctgggcgtg attgaactta
     2941 agcgttcgtc agtgcacgtc agcgaaggca tccggcagaa tctcgataat caaaagaaaa
     3001 cctttatccg cgatttcttc accactatgc agttagtgat ggcaggcaac gatacgcagg
     3061 gcctgcgcta cggcaccatc gaaaccccgg aaaaatacta tctggagtgg aaagaagata
     3121 atcctggcga ctatacacat aaactcgact ttcatttaag tcgtctgtgt agcaaatcca
     3181 ggctgttgga tatcgtccat aattacattg cctttgatgt cggggtgaaa aaactctgcc
     3241 gccataatca gttcttcggc gtcgaggcgg ctaaacaaca tattgcccgt cgtgaaggcg
     3301 gtattatctg gcacacacag ggatcgggta aaagcctgac gatggtctgg ctggcgaagt
     3361 ggatccgtga aaatgtgcca aactcccgcg tgttgattgt gactgaccgc accgaactgg
     3421 atgaacagat cgaaagcgtg tttatgggcg tgaatgaaga tatttatcgc acctccagcg
     3481 gcaacgatct tatcgcaacg ctcaatcatc ctaacccgtg gcttatttgc tcgctggtgc
     3541 ataaatttgg tcgtcgcagc gaagctgaag acaatgcggc taccgacgcc tttatcaccg
     3601 aattacagca gtccctgacg aaaacattct gcgccaaagg cgatctgttt gtttttgtgg
     3661 atgagtgcca ccgcacacaa tccggcaagc tgcataacgc catgactgcc attctgccgg
     3721 atgccctgtt tatcggtttt accggcacac cgctgatgaa gaaagataag aagaagtcgg
     3781 ttgaggtgtt cggcccttat atccatacct ataaatttga tgaagcggtg gccgacggtg
     3841 tggtgctgga tctgcgctat gaggcgcgcg atattgacca gtatctgaca tcagagaaaa
     3901 aggtcgatga ctggtttgaa gccaagactc gcggtttgtc gaatttagcc aggacccagc
     3961 ttaagcaaaa gtggggatca atgcagaagc tgctctccag caagtcgcgg ctggagcaga
     4021 tcgttaacga cattttgttg gatatggata cccgtccacg cctgatggac gggcgcggca
     4081 acgccatgct ggtatgcagc agtatctatc aagcctgcaa ggtttacgag atgttcagcc
     4141 agaccgagct ggcaggaaaa gtagcgattg tgaccagcta tcgccccgat gccgccagca
     4201 tcaaaggcga agaaacaggc gaagggttaa cggagaaact ctctaaacac gccacctatc
     4261 gtaaaatgtt ggctgactat tttgaacaaa gtgaagagaa ggccgccgca cgcattaccg
     4321 agttcgagcg ggaagtgaaa cagcgtttta ttgaagagcc ggggcaaatg cgcctgctta
     4381 tcgtggtgga taagctgctg actggctttg acgcgccgtc cgccacctat ctctacattg
     4441 ataaaaccat gaccgaccat acgctgtttc aggcgatttg tcgggtgaac cggctggacg
     4501 gtgaggataa agagtatggc tatattatcg attataaaga tctgttccgt tcgctggata
     4561 aagcgattac cgattacacc acgggcgcgt ttgatgatta cgatcgggac gatgtggccg
     4621 ggttgctgaa agacaggctg gagcaggcgc aactggatct cgacagcgcg ctggagatgg
     4681 ttcgcagctt atgtgaaccc gtcaaaaccc cgcgcagcct gcaggactat cagcactatt
     4741 tttgcggtaa atcaggagag aatcagactt cgttaaacga aaaagaggcg ctacgcctga
     4801 cattttacca gtgcgtcgcc catctgctac gcgcttatgc cagcatcgcc aatgaaatgc
     4861 cggaagcagg ctacactgcc gaagaggtag agagcattcg cgccgaagtc gctgaattcg
     4921 aaaaactgcg ctatgagatt aagctgacca gcggcgatct tctggatatg aagcgctttg
     4981 aacccgctat gcgtcattta ctggatatgt atgttcgcgc cgatggtagc gaagtgctga
     5041 tggattttga agagttaagt ctgattgaac tgattgtcga gaaaggcttg gcggcgaccg
     5101 tcgccctgcc ggatgatatc cgtaataatc aggatgcgat ggcggaaacc atcgaaaaca
     5161 acgtgcgcaa aaccatcgtg gatgaaaacc cggttaaccc aaaatactat ggacgcatgt
     5221 cgcagctgct ggatgaactg attacccttc gccgacaaaa tgcgctcaat tatcagcagt
     5281 atctggaacg tatccgcgat ttgtgtaaac aggtcatccg cccggagcaa accgccggag
     5341 cgtgctaccc tgccaccatg gatacacaag caaagcgcgc cttctatgat aacttcggcc
     5401 acgacgaggt actggcgacc cgcatagata cgacgattcg ctataccaaa agggcagagt
     5461 ggattggcga tcgttttaaa gaacgggaaa ttgccaacgc attacgtgaa gagaccgcca
     5521 gctataatat tgatattgat gaggtgatag cgcttgcccg tcagcagaag gaatatcact
     5581 gatgccaatc atgcaaatag gctcgttaca cttgctggtc aaccgtaagg cgattaagaa
     5641 tctgcatatc agcgtgctgc cgcctgatgg caaagtccgc gtttcagcgc cggaacatat
     5701 gacggagacg gcgattcgta tggcggttgc ttcacgcttt cgctggatca gaaaacagca
     5761 gcaggatttc tccagacagc ctcgtcaatc agagagggaa atggtcagcg gagagtgcca
     5821 ctatctttgg ggccgaagat atcgcctact tgtgattgag cgtcacgggc gtcacgaagt
     5881 aaaagttgca ggaaataata agctccagct ttatgttcaa ccgggaaccc gtgatgaaaa
     5941 taaggcattg gcgttaagtg aattttatcg acatgaacta aaacagcagg tggctaagct
     6001 tctacctgaa tggcaggacc gaatcggtgt tcccctaaca ttctggggca tcaaaaagat
     6061 gaaaaccttt tggggcacct gtaacactac aacaaaacga atctggctca atctggagct
     6121 aattaagaag ccgccggagt gtctggagta tattctggtg catgaactgg ttcatcttct
     6181 ggaacgtcac cataatgctc gcttccggtt gcatatggat cggtttattc ctgactggcg
     6241 tgagcgtcgg gatttgttaa ataagatgcc gttagcgttt gaggcatggg agtattgatt
     6301 gagcctttaa ctgttttttg gtaagcgtaa ccctgcttgt tcacgtaaaa tttttgccgg
     6361 atcgcttgct cccttcgccg gttcacacca gcagtcttca caggcgttac aaaatttaca
     6421 acgttaactg ggattaatag ccggaggtga ggccggaaaa cgggcagcaa cggcagtggg
     6481 agctgagcgc attattctca acaacaaatc gtcccttatc ttcacttaaa atccttttat
     6541 gccaagaaag cgaaacaaga ctataccaaa acctagaatt ccacctccca cacaccccat
     6601 ccttagacct cttaaaactt cggacaatct aaaatcaact tccccatacg ctaagtattt
     6661 aatgataagc aatatgaaat aagacaactc agcaattgca attcctgaaa ccatggaggt
     6721 aactatgaca aagagactga aaataaatag tttcattcac gcactccttt cttcaattta
     6781 tcattaatga ctttttgtag ttcagtcccc gtcatttctg atgtaataga agcccccata
     6841 ttacctgcag cccccggaat cttgctcggc accatttctt tggagatacc taacttccct
     6901 ttaacttcag tatattttag tagagtcgga tcgaatttag gattccagcc acctgttatc
     6961 cacttgcctg ctgtatttgc aacaggcttc accacataat tgccaacccc atatccaaac
     7021 ccggcccccg ctccatttgt aatcgaccca gtaagaggat catctccgtt tatagcgttg
     7081 gttaatgctc cacctgccgc attccaagct attgtccctt tccagccctg ccccatggtt
     7141 atcacattta tccagcctgc tgccactgag ttgaccgggt ttatttcacc actatctgca
     7201 tactgcatcc cggcatttgt cccggcacca agtaaccaca tagcctggac actccccggt
     7261 aacagggcaa cgctgcctgt tgctaatgcg gctgcataag ctttattttc atcctgcccc
     7321 cagaaccaaa caaactccga tagagaaaac gcaggcatag atgaatagtt tcaggtaact
     7381 aatcattttt tgcccccgat ttttcttcag atgggttatt taatgcttct ttcgtgaatc
     7441 cgttaataaa ctcacgacca acacctccga tagcatcata aacaaaatta ggaatgttat
     7501 tcgcatttaa tactttatct acaccaactg gcgcatactt cccaaacgcc ccacccagac
     7561 cagcaccaat accagccccg gctagtgaag ctgtatttgg accatcagta aagaatgccc
     7621 cacccattgc gatacctgtg tttgcaagga caccacgacc gggagccatc atgcctgttg
     7681 taaatgaggt cgcgcttccc agatagtccc acgacttatt cgcattctgc ggctggttca
     7741 aatcatagaa ctgataacca ctattggccg ccagtccaag aagcccgcca ccaagaactt
     7801 tacctgctga cgcagcaggc cccagatacc atgccccggc aatcattaca ccatcctgat
     7861 atccatccac gatcactttc gtgatattga tgctgtccgg catatcacct ttcgctatct
     7921 tatccagccc agcctgggtt tgttccggtg tcagattatt gcccaccgcg tacttgttcc
     7981 atgatgtagc cgcagcagca ttgctttcaa aaccatcacc gaagctgagc gcgttattct
     8041 caacgacata gctatcgaca aaaaaccata ttaattcttt tggaaaaaag aaaataaggt
     8101 atccatataa caacaccaaa tattccactc actaataatc tgatattatc tgaatccagc
     8161 tgtacgtcaa aaagaatagc cggtaataaa gtaagataga gagcaataat cagtcctgtt
     8221 gcataataag cgatcatggc ttttcgagtt ccttttttgc gtctgaaaaa caaccacgtt
     8281 gcataaaggg aaacaatgaa tccacatatc agcaacgcaa atgcccccat agcgtaataa
     8341 gataaaaagc cagtttcatt gaaataattg aatgcggtta tggtaaaact gtaaagttca
     8401 atagtactac caataacacc caagataagt cccagagcag gaagatataa tagtccatta
     8461 attttactta actcctcatt ttcacaggca tcacacaatc ccgactcttt gtttgcttct
     8521 gacattttac attttataca ttccattttt tattccatta ttcattgcgt tacgataata
//
LOCUS       DAZFFG010000161         8575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFFG010000161 DAZFFG010000000
VERSION     DAZFFG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.162"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..186)
                     /locus_tag="ACHJ7J_004452"
     CDS             complement(<1..186)
                     /locus_tag="ACHJ7J_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002591.1"
                     /translation="MNAKNFNQLYPVGTRFTHTDSAVFGCGRVVKTVAPARDFKCGCV
                     VEINVEPYFVKVETLKAE"
     gene            complement(167..400)
                     /locus_tag="ACHJ7J_004453"
     CDS             complement(167..400)
                     /locus_tag="ACHJ7J_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO5002592.1"
                     /translation="MHQDIKEYRAGNSCAAYSLGASRAEQRGDYAEAEKLWRKAAQSP
                     CSTLRRIWAEHRAQFCANAHLKGWRPPHEREEL"
     gene            complement(402..1046)
                     /locus_tag="ACHJ7J_004454"
     CDS             complement(402..1046)
                     /locus_tag="ACHJ7J_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HFO5002593.1"
                     /translation="MTTENKVKQYTKTQAPEGYWVDARGVMTPESLIKDIDRDREQLV
                     GELVEMVIAASASLRELKLRAFGDIQAFIDLSAEKYGAVKGGKKGNITLYSFDGRYKI
                     QRAMQDRIAFDERIQTAKSLIDDCLADWTEGARPEIKAIISEAFSTDKEGNINTGRVL
                     ALRRLEIEDERWNNAMTLIGEAVQVIGSKSYIRVYERVGDSDEYRAIPLDIAGV"
     gene            complement(1076..1291)
                     /locus_tag="ACHJ7J_004455"
     CDS             complement(1076..1291)
                     /locus_tag="ACHJ7J_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002594.1"
                     /translation="MAVKLEVIIYTDENGTLRVRSMGAIDKKGFTDKEVGVRQRLWEV
                     MNQTLNKEFNGVDLFSVGVEPCEHNTH"
     gene            complement(1304..1528)
                     /locus_tag="ACHJ7J_004456"
     CDS             complement(1304..1528)
                     /locus_tag="ACHJ7J_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002595.1"
                     /translation="MDKQTINAVRSRARVAIHTTGGRVTGQHHRLPVVYACPPVGGPV
                     WPVTEIIQRFSGLRRVVKTSCIDGCTVVWQ"
     gene            complement(1543..2436)
                     /locus_tag="ACHJ7J_004457"
     CDS             complement(1543..2436)
                     /locus_tag="ACHJ7J_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5002596.1"
                     /translation="MSLITQLNDVMARRGYTQTHVARAIGRSSAVINQYLQGKYQGDM
                     ADIEERISAFVTREREKENSRRIKAKFVTTGMAAKGLEVLAYAHLECEICVLYGAAGL
                     GKTMILREYASRNRDAVLIEADPGYTARTLLEELCRQLGVKVRGNIHELIDACVRELR
                     DSGRLLMVDEAELLPYRALEVLRRLHDKAGIGVVLAGMPRLLINLKGRRGEFAQLYSR
                     VALALNLGDTLAQEDFNQIATGLMPEAAEPEISEALYARSLGNARRLFKLARGVYRIC
                     DISDAPVSVQAIDKFAEMLIH"
     gene            complement(2455..4497)
                     /locus_tag="ACHJ7J_004458"
     CDS             complement(2455..4497)
                     /locus_tag="ACHJ7J_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO5002597.1"
                     /translation="MFYSANELAGLPGLPGTVQGVRWTLNRITETHPEWKRKREGTKA
                     FEYHIDCLPAEAQKVLRKRLTHQVLEDAQLPAVVEQKAVKNVAVRDELEVMVKCPELA
                     LREVQSLTDKQKAIADARILLATEVHKLREYAGMTRKAALKHIVDGVRMGALPDRIIE
                     AANTANARQGKRTGVSTGSLDSWYSSWVMARGDANQLLALLAPGHHKGTPWEQVWWLS
                     DFFMFYRSWKRPTVEYAYREFSAWWHEKHANDAGMLAALPSVHAVRRVLSSVPVIVKE
                     RFRSTGSAWRSLNPFVRRDWTSLPVNAVWVGDGHCMKMTAFNPLTGNIFRPEVTLVMD
                     AGQRFVVGWSLSLSENVIAVADALRYGMAQHGIPLIYYSDNGGGEKNRVLDADITGIL
                     PRLGVEHHTGIPGNPQGRGVIERANKGIPKDVALSFQTYCARDADKETVMMQQRIMQS
                     AIKATHKGKELTKRQVKARDEIPTFEQLMAAIELEVRRYNNRPHSSLPRKEDGEYYSP
                     AAYRRKLIKEQNVEIDFLSPEELHEMFRPEVTRKTFRGEIQLFNNIYYSYELAAEHGN
                     EVRVSYDIHDANSVIVRRADGSYICDAIWNGNKVDAFAKPVIEQKLEKRVRGRIARAT
                     QKIEEAKRELTPAITQKPDFNFGFGLERPEKKEKEELYLFASERERNLKKNGTNNR"
     gene            complement(4497..4733)
                     /locus_tag="ACHJ7J_004459"
     CDS             complement(4497..4733)
                     /locus_tag="ACHJ7J_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5002598.1"
                     /translation="MMVRNEVMNKDWHREYIVAAVHTKGFTLRELSERAGLKKDSLKN
                     ALYRSCPKYERIIADAIGVEPAEIWPSRYASKAA"
     gene            4916..5563
                     /locus_tag="ACHJ7J_004460"
     CDS             4916..5563
                     /locus_tag="ACHJ7J_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5002599.1"
                     /translation="MTRNDDAFSFVDRGKESFRERLVKLIGNRSVRTAASDWGIPPST
                     LNNYIHKGTEPSFKIACLISDKEQVSLNWLAYGEEPIAKESSLSNQTAQPSPDITNEL
                     VNELLKRASTEDKEQLISAICDMGIKGILERLQQPTRQTKQADSELEEQRKAIQSLNI
                     RQSLKDAVCMALAGDEENDKEILRRAKAIFCAGTPGDRVPVTPEQETKPVNKKSA"
     gene            complement(5875..6780)
                     /gene="ssuD"
                     /locus_tag="ACHJ7J_004461"
                     /pseudo
     CDS             complement(5875..6780)
                     /gene="ssuD"
                     /locus_tag="ACHJ7J_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(6777..7736)
                     /gene="ssuA"
                     /locus_tag="ACHJ7J_004462"
     CDS             complement(6777..7736)
                     /gene="ssuA"
                     /locus_tag="ACHJ7J_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO5002600.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(7729..8304)
                     /gene="ssuE"
                     /locus_tag="ACHJ7J_004463"
     CDS             complement(7729..8304)
                     /gene="ssuE"
                     /locus_tag="ACHJ7J_004463"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO5002601.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALNTFTEQLQQADGLIVATPVYKAAYSGVLKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
BASE COUNT         2067 a         2312 c         2044 g         2152 t
ORIGIN      
        1 ctctgctttc agtgtttcga cttttacgaa ataaggctcg acattaattt caaccacaca
       61 accacattta aaatctctgg ctggcgcaac agttttcacc acacgaccac aaccaaacac
      121 tgcgctgtcg gtatgtgtaa aacgagtccc caccggatat agctgattaa agttcttcgc
      181 gttcatgagg tggcctccat cctttcaggt gagcattggc acagaactgt gcccggtgtt
      241 cagcccagat gcggcgcagg gtgctacacg ggctttgcgc tgccttgcgc cacagttttt
      301 cagcctcagc ataatcgccg cgttgttctg ctctggatgc acccagcgaa tacgctgcgc
      361 aactatttcc ggctctgtac tctttgatat cctgatgcat gttaaacccc tgcaatatcc
      421 agcgggattg cgcggtattc atcagaatca ccgacgcgct catacacgcg gatatagctt
      481 ttgctgccaa tcacctggac cgcttcgcca atcagcgtca tggcgttatt ccagcgttcg
      541 tcctcaattt cgaggcgacg cagggccaga acgcgcccgg tattaatgtt gccttccttg
      601 tccgtgctga atgcctcgct gatgatggct ttgatttccg ggcgcgcacc ttccgtccag
      661 tcagccagac agtcatcaat cagcgactta gcggtctgaa tgcgttcatc aaacgcgata
      721 cggtcctgca tggcgcgctg aatcttgtaa cgcccgtcaa agctgtaaag cgtgatattg
      781 ccttttttgc cgcctttcac agcgccgtat ttctccgccg acaggtcaat aaatgcctgg
      841 atatcaccaa acgcacgcag tttcagttcc cgcagtgagg cggaggcggc aatcaccatc
      901 tccacaagct caccgaccag ctgctcacgg tcgcggtcaa tatctttgat aaggctttca
      961 ggtgtcatta cgccacgggc atccacccag taaccttcag gcgcttgagt tttcgtgtat
     1021 tgcttaactt tattttcagt cgtcataatt aaattacctc agtgatattt acagattaat
     1081 gtgtgttatg ctcgcagggt tccacgccaa cagaaaataa atcaacgccg ttaaattctt
     1141 tattaagcgt ttgattcatt acctcccata accgttgtct tactccgacc tctttatccg
     1201 taaatccttt tttatcaata gcccccatac tcctgacacg aagcgtgccg ttttcatcgg
     1261 tgtagataat tacttctaac ttgaccgcca ttctgatttc ctctcattgc catacgacgg
     1321 tgcagccgtc gatacaggat gttttcacca cgcggcgcag gccgctgaat ctctggatga
     1381 tttccgttac cggccataca ggaccaccca cgggcgggca ggcataaacc acaggtaaac
     1441 ggtgatgctg tcccgttaca cgaccgccag tggtgtggat ggccacacgg gcacgggaac
     1501 ggacagcgtt aatggtctgc ttgtccatcg ccttgttctc catcagtgaa ttagcatttc
     1561 tgcgaattta tcaatagcct gcacactgac cggtgcatcg ctgatatcac aaatccggta
     1621 cacaccgcgc gccagtttga acaggcggcg ggcattaccc agcgagcgtg cgtaaagcgc
     1681 ctcgctgatt tccggttctg ctgcttccgg catcagcccg gtggctatct ggttaaaatc
     1741 ttcctgcgcc agcgtatcgc ccaggttaag cgccagcgcc acacggctgt ataactgggc
     1801 gaactcacca cgacgcccct tgaggttaat cagaaggcga ggcatacccg ccagaacaac
     1861 cccgataccc gctttgtcat gcagacggcg cagaacctcc agtgcgcgat aggggagcag
     1921 ttcagcctcg tccaccatca gcagacgacc ggaatcacgc agttcgcgca cacaggcatc
     1981 aatcagttcg tgaatgttgc cgcgaacctt tacccccagc tggcggcaca actcctcaag
     2041 cagtgtcctg gctgtatagc ccgggtcagc ctcaatcagg accgcatccc ggtttctgga
     2101 ggcgtattca cgcagaatca ttgttttgcc aagacctgcc gcgccataca gcacacagat
     2161 ttcacattcc aggtgagcgt aggccagcac ctccagtcct ttggcagcca taccggtggt
     2221 cacaaacttt gctttaatac ggcggctgtt ttccttttcc cgctcacggg tcacgaaggc
     2281 agaaatgcgt tcttcaatat cagccatatc gccctgatat ttaccctgta aatactggtt
     2341 gataacggcg ctgctacgac caatagcacg ggcaacgtgg gtctgcgtat agccgcgacg
     2401 ggccattacg tcatttaatt gcgtaattaa actcataatt aaataccttt taaattaacg
     2461 gttgtttgtg ccgtttttct ttaaattgcg ttcgcgttca gatgcgaata aataaagttc
     2521 ttctttttcc tttttctccg ggcgctccag accaaatcca aaattaaagt caggtttctg
     2581 cgtaatagcc ggggtaagct cgcgtttagc ctcttcgatt ttctgcgtgg cccgtgcaat
     2641 gcggcctctg acgcgtttct ccagtttttg ctcgataact ggtttagcga aggcatcaac
     2701 cttgttaccg ttccagatgg catcgcagat ataagagcca tcagcccgcc ggacaatgac
     2761 gctgtttgcg tcatgaatat cgtaactgac cctgacttca ttcccgtgct ctgcggccag
     2821 ctcatacgag taataaatat tgttgaataa ctgaatttcc ccgcgaaatg ttttgcgcgt
     2881 cacttccggt ctgaacattt catgcagttc ttccggcgac aggaaatcaa tttcaacatt
     2941 ctgctctttt atcagtttcc ggcgatacgc tgccgggctg taatattcgc catcctcttt
     3001 gcggggtagg ctgctgtgtg gccggttgtt atagcgtctg acctccagct ctatggctgc
     3061 catcagttgc tcaaaggtag ggatttcatc cctcgcctta acctgccgtt ttgtcagctc
     3121 tttccctttg tgggtggcct taatggctga ctgcataatc cgttgctgca tcattacggt
     3181 ttccttgtct gcatcccttg cgcagtacgt ctggaaactc agagccacgt ctttgggtat
     3241 tcctttgttt gcccgttcaa tcaccccgcg cccctgcggg ttacctggta tccccgtgtg
     3301 atgttccaca cccagccggg gcagaatccc ggtaatatcg gcatcaagca cccggttttt
     3361 ttcaccgccc ccgttatcgg agtaataaat cagcggtatc ccgtgctgcg ccatgccgta
     3421 acgtaacgca tcagcaacgg ctatcacgtt ctccgacagg gacagtgacc agcccacaac
     3481 aaagcgttgc cccgcatcca taaccagcgt cacttctggc cggaaaatat tgccggtcag
     3541 ggggttaaac gcggtcattt tcatacagtg accatccccg acccataccg cattaaccgg
     3601 taatgatgtc cagtcgcgcc gcacaaaggg attgagcgaa cgccaggctg aaccggtgga
     3661 gcggaaacgc tctttaacaa tcaccgggac gctgcttaac acccgtctta ctgcgtgaac
     3721 tgaaggcagt gccgccagca ttcccgcatc attcgcgtgt ttttcatgcc accaggctga
     3781 aaactcacga tacgcatatt caacagtcgg gcgcttccat gagcgataga acataaagaa
     3841 atcgctcaac caccagacct gctcccacgg tgttccctta tgatgaccgg gagccagtaa
     3901 cgccagtaac tgattggcat caccgcgcgc catcacccag ctggagtacc agctatccag
     3961 gctgccggtg ctgacaccgg ttcgctttcc ctgacgtgca tttgcagtat tcgctgcttc
     4021 gataatgcgg tcaggtaacg cccccatgcg cacaccatca acgatgtgct ttaaagcggc
     4081 cttcctggtc attccggcat actcgcgcag cttatgcact tcagtagcca gcaatatccg
     4141 cgcatcagcg atggcctttt gcttgtcggt cagtgactgg acttcgcgta atgccagctc
     4201 cgggcatttg accatcacct ccagttcatc gcgaacggcg acgtttttaa ctgctttctg
     4261 ctcaactacg gcgggcagtt gtgcatcttc caggacctga tgagtcaggc gcttacgtaa
     4321 aactttctgc gcttcagcgg gcaggcagtc gatgtgatac tcgaatgctt tggtgccttc
     4381 gcgcttgcgc ttccattctg ggtgcgtctc tgtgatccga ttgagcgtcc agcgcacccc
     4441 ctgaactgta ccgggcaaac caggaagacc ggccagctca ttggctgaat aaaacattac
     4501 gccgccttac tggcataccg gcttggccag atctccgcag gttccacacc aatagcatcg
     4561 gctatgatgc gttcatattt agggcatgag cgatacagtg cattttttaa agagtctttt
     4621 tttagaccag cgcgttcaga aagttcacgc aatgtaaaac ctttggtgtg cacagcggca
     4681 acgatgtact cgcggtgcca gtctttgttc attacttcat ttctaaccat cattaggtta
     4741 cccttaaaag ttatccgtgc ggataacacc gccgtgttat cccttcggat ataagtattg
     4801 atccatatat agcccatgta aagcaaaaat agccctttct ttgtattttc tttttgattt
     4861 tggtctttat atggtttttg caattaaaat caatgagtta tagaggaaag aaaaaatgac
     4921 tagaaatgat gatgcttttt cttttgtcga ccggggaaaa gaaagtttca gagagcgttt
     4981 agtaaagctg ataggtaacc gtagtgtcag aactgctgcg agtgattggg gaatcccccc
     5041 ttcaacctta aacaactaca ttcataaagg aacagagcct tcttttaaaa tagcttgcct
     5101 tatctcagat aaagaacaag tgagcttaaa ttggcttgct tacggtgagg aacccatcgc
     5161 caaagaaagt tctctatcaa atcaaacagc gcagccttct cccgatatta caaacgagct
     5221 agttaacgag ttattgaaaa gagcgtccac agaagataag gaacagctga taagtgcaat
     5281 atgcgatatg ggcattaaag gaatcttgga acgcctacag caacccacca ggcaaactaa
     5341 gcaagccgat agcgaacttg aggagcaaag aaaggccatt caaagcctga acatcaggca
     5401 atcgcttaaa gacgctgttt gcatggcatt agctggcgat gaggaaaatg acaaagagat
     5461 tttgcgccgc gccaaagcca ttttctgtgc tggaacacca ggtgatcgcg ttcccgtcac
     5521 accggagcaa gaaactaagc ctgtaaacaa gaaatcagca taagcgcctc gttttggcgg
     5581 ggcgtgtgct gttagctaat cagcaataac cacaacaaaa ccgaacacaa cagatgagaa
     5641 caaattgagg aaaacgggta aggataaaac tctttttaaa agcatttaaa acccgttaag
     5701 tttgtagcag aaccagatca aaagcgccca gaaaaaagcg caaaaaaaag cgtttttggt
     5761 catttctcac aaatccgccc agaatcacgc taaatcacca caccccgcgc cacacaaggc
     5821 ttgccggttc taccaggctg cactaactcc gcccagaatt gaacactacc ccacaccggc
     5881 accgccgcgc actaagccaa cgcccgccca taaattgggg ctgatctcca gattgtcgcg
     5941 cttgccgtta tgtagcgctg ccattcgctg ttgccccacg gaatccgtgc gggcgaatgc
     6001 ggcttgtgct ttggcgatag tctcatcatc aagatgcgag attaaccgct cggcggcctg
     6061 ccacgcttcg tcgttagttt cacgaacaat cacatgcaga cgaatgccga aacgaatttt
     6121 gcgtccatgc gcggcagctt tcgcccgcac ttgttcgatt ttctctttaa ccagttccgg
     6181 cggttcgccc caggtgaggt agagatcaac ctgttctgcc gccagctcct gggcgatgtc
     6241 tgacgatccg ccaaagtaaa gcggaggata cggctgttga atcggcggga agagcagttt
     6301 ggctccccgc acatgaatat gtttaccgtt gaaatcgacg gtttctccca gcaataagcg
     6361 ccgccagacc tgggtaaatt ccgccgaggc ttcgtagcgc tcactatgat caaggaacac
     6421 gccgtcgcct gccagttctt gtggatcgct gcctgtgacc aggttaaaca acgcacgtcc
     6481 atttgagaga cggtcaagcg tggcggcctg gcgggcggca acggtaggtg aggttacgct
     6541 gggacgcagg gcgacaagaa acttcagccg ctgcgtcacc gggatcatcg atgcggcaac
     6601 cagccacgca tcttcgcagg agcgccccgt tgggatcagc acaccggtat agccaagacg
     6661 atccgccgct tgcgcaattt gttgcagata accgtgatca accgggcgtg aaccttcttc
     6721 cgttcccaga taatgcccgt caccatgggt cggtaaaaac cagaacatat tcagactcat
     6781 aattgttttc cttccagttg agtgggctgc cagatgcgtt ggcgaatatc gacttttttc
     6841 ggcaccagac ggttttcata aaacagatct gccgtttgct gctgtaaggc ggcaacctcg
     6901 gcgttaaccg gtttgatggt ggtaggaggg cgatgatcta agtaagaggc aatcaccggt
     6961 gccggtaagc ccatcgtttt tgccagtaaa gcgatgcttt gctcgcgctg gctgcgggtt
     7021 aacgcatcgg cctcactaaa ggttgccagt acaccctgaa taaaagcgcc gtttttttct
     7081 gcataggggc gagctgccag ataaaacgat ccggtttgat tgagatcggt gccgtctttc
     7141 aacacccgca cgccgccctg taataatgca gcggagtagt agggatccca gatagcccag
     7201 gcgtcaacgt taccttgctg gaacgcggcg cgggcatcag ctggcgtcag ataagtgggc
     7261 tggatgtcag taaacttaag tccggcctgg cgtagtgcgc gcagtaaaag attgtgtgaa
     7321 ctggaacctt tctggaacgc aactttgtgg cctttaagat cggctacggt tttgatcggg
     7381 ctgttttctg ccaccagaat cacctcggct tttggcttcg gcggctcgac gcccacgtac
     7441 agcaaatcag ccccggcagc ctgggcgaag attggcggaa tatccccggt actgccgaga
     7501 tcaatactgc caacgtttaa cgcctcaagc atttgcgggc cagcggggaa ctccacccag
     7561 gagatttttg attgcggata gcgtttttcc agtaactggt ggctttttgc caataccata
     7621 ccaatactgc ctttctgata gcctatacgt aacgcttcag gcgaggattc tgcagcaacc
     7681 acaatcgtag agacgctaag caatcccacc agcgccagtt taatgatgtt acgcatgggc
     7741 attacctcgc agagaatgaa gatcaggaac ctgaacatcg cggcggtgca atgcctgcca
     7801 gaaagtttct agcgcggtat caagacgggt ttgtaaattc ggcgtgaact ggggtttgtg
     7861 atgataatca attacttgtg agtcatcggc aaacacgccg tgcaggatct cctgggcttt
     7921 cagcgcactt aaaactggct taagggcata atcgaccgcc agcagatggg ccaccgtacc
     7981 gcccgtcgcc agcggtagca ccactttgcc ttgcaacgca cgttctggca gcaggtcgag
     8041 cagggttttc agcacaccgg aataagcggc tttatacaca ggcgtggcga caatcagccc
     8101 gtcagcctgt tgcaactgtt cggtgaaggt attgagtgcc ggactatcga aacgagcata
     8161 aagcagatct tccgcggcga agttttgcag attccagtga taaacctcta catccaggcc
     8221 atttagtttt tcccgcgcat attccagcaa ggagctggag cgagaaggaa agcgaggact
     8281 acccgccagg gtgatgacac gcatactctc tccttataac caattgttct tgttttgtta
     8341 acattgataa caattcgctc agtctgtcgg agagacaaga aaatgccaaa tataaatttt
     8401 gtgtatcttt ttctgaaaat aaactaaaga aagggcttta tatatcacgc atatttattt
     8461 attggatagt cattagatgt tgaatcaatg aattactcgt tcaattaata tttaaatatt
     8521 catgaaatct ataaattaaa gatttgtcac ttattggatt tagtatgctt gattt
//
LOCUS       DAZFFG010000162         8551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFFG010000162 DAZFFG010000000
VERSION     DAZFFG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.163"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1971
                     /locus_tag="ACHJ7J_004464"
     CDS             <1..1971
                     /locus_tag="ACHJ7J_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFO5002602.1"
                     /translation="SVAEQTGMFAGDGGFDITVGRHTQLDGAVIASTATADKNSLDTG
                     TLGFSDIHNEADFKTQHSGISISGAGSFGDQFKGNMPGGMISAAGNSGHAEGTTQAAV
                     ADGTITIRDKDNQKQDVANLSRDTEHANDSISPIFDKEKEQNRLKEIGMISDIGGQVA
                     DIARTQGELNALKVAQDKYGPLQADATEEQRQAYLAKLRETPEYKKEQEKFGTGSDIQ
                     RGIQAATAALQGLAGGNLAGALAGASAPELANFIGHNAGINDNDAAKAIAHAILGGVT
                     AALQGNNAAAGSVGAVSGELIAGAIAKVLYPDEKDLSKLTEDQKQTLSTLASISAGMA
                     GGVAGGNTAGVATGAAAGKNVVENNLLGGNEDTQTKFVQEHGKDIASCSTNPGGAECK
                     RGEAVNKAIAAALVGGATGGVVIALTPEAIAAAQAAVSACGANTVLCANEVSIWVAEM
                     VAGDALPAGLTVAAVGKMSASELSELKALMAVEKQTGQKISAESLEKLMLDSGGKGNW
                     SKELNNPQPNKVYHVDGDKIYKTDAQARPSQVEATLSPNVKDRNGYQQCKAGKCGLDG
                     DEGGHLIASIFNGPGEKLNLVPMDGNLNKGAWKQMENTWAKALGEGKEVKVNIQPSYI
                     GDSVRPAKFKVVYSIDGGRPIEKVFNNAPGGK"
     gene            1971..2303
                     /locus_tag="ACHJ7J_004465"
     CDS             1971..2303
                     /locus_tag="ACHJ7J_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein YezG family protein"
                     /protein_id="HFO5002603.1"
                     /translation="MRSDQDIYNDIGSVLLSITPSDAVKIILCAKLEPESDCGEFTYD
                     YVDKQGNQHWITETADASERLLDLLVELRNLFVDNFKSQEKPFWHGCEVTVNIETLKI
                     NIDFKYEN"
     gene            2400..5813
                     /locus_tag="ACHJ7J_004466"
     CDS             2400..5813
                     /locus_tag="ACHJ7J_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5002604.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPLLTQSSGRADEKRLEDAIRAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEDLKNTDQVTQEHPVKEQPTATGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPIIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNSVAHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTYTTEFLREGIKREKLS
                     DAQIRELEEQGMDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAMRFDMDILAAQTARYTNPEELDVLWPVIVEKVERLPPHLAQVQQQGPRINQLRD
                     LGWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQSELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIEQIARLQKIVEELGVVTDAATV"
     gene            5877..7511
                     /locus_tag="ACHJ7J_004467"
     CDS             5877..7511
                     /locus_tag="ACHJ7J_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5002605.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVEAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLEAVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKYYRTLRG
                     LPVDNFMGEKSASLLKQQHPEGIDVGVDFKDRTQAAFVVPKEDIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            7508..>8551
                     /locus_tag="ACHJ7J_004468"
     CDS             7508..>8551
                     /locus_tag="ACHJ7J_004468"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5002606.1"
                     /translation="MSWPKVKLGDVAPAIPVKNVNLDEDELVWQLNLDQIESNTGKIV
                     DKVKQPLSSAGSSTHFFNNEHVLYSKLRPYLNKVVVPDELGIATTELVPMKPDQKRLN
                     KEYLAYYLRSKPFVEWVSTQVSGAKMPRVVMSIFWQHEMPLPPLYEQERIVSVLDKSN
                     RLRQKREQAIKLADDFLRATFLEMFGDPIENPKGWNVKPLADQIVHANNGISRRRKED
                     TNEGDIVLRLQDVHYSGIAFDKELNRIKLVDKEKQIARVEHDDLLFIRVNGNPNYVGR
                     TAVFKSYIEPVYHNDHLIRIKLDNEYQSDFLCYLINSPFSRKLIAQQIKTSAGQHTIS
                     QDGILKLMFYRPPI"
BASE COUNT         2392 a         1923 c         2267 g         1969 t
ORIGIN      
        1 tccgttgctg aacaaaccgg aatgtttgcc ggtgacggcg gcttcgacat tacagtgggc
       61 agacataccc agctcgatgg cgcggttatc gcttccacgg cgacggcaga taaaaacagc
      121 ctcgataccg ggacgctcgg cttcagcgat attcacaacg aagcggattt caaaactcag
      181 cactcgggta tcagcatcag cggagcgggc agcttcgggg accagttcaa gggcaacatg
      241 ccgggcggaa tgatttcggc ggcaggcaac agcggccatg ccgaaggcac gactcaggcc
      301 gccgtggctg atggcaccat caccatccgc gacaaggaca accagaagca ggacgtggcg
      361 aacctaagcc gagacaccga acatgccaat gacagcatca gcccgatctt cgacaaggag
      421 aaagagcaga accgcctgaa ggaaatcggg atgatcagcg atatcggcgg ccaagtggcg
      481 gatattgccc ggacgcaggg agagctgaat gcgctgaaag tcgcgcagga taaatacgga
      541 cctcttcagg cggatgcgac ggaagaacag cgacaggcat atctggcgaa actgcgtgaa
      601 acgccggaat acaaaaaaga gcaggaaaag ttcggtactg gtagtgatat ccagcgtggc
      661 attcaggcgg caacggccgc gcttcagggc ctggcggggg gtaatttagc aggtgctctg
      721 gcgggggctt cggctccgga actggcaaac ttcatagggc acaatgctgg aattaatgat
      781 aacgatgcag cgaaagccat tgctcatgcc attctcggcg gtgtgactgc ggctcttcag
      841 ggaaataatg ccgcagcagg ttcagtcggt gctgtgagcg gtgaacttat cgcaggtgca
      901 atcgcgaagg ttttatatcc ggatgagaaa gacctgtcga agctgaccga ggaccagaag
      961 cagacgctaa gtacgctggc gagtatctca gcaggtatgg cgggtggtgt tgcaggtggg
     1021 aatactgcgg gtgtggctac cggagctgct gctgggaaga atgttgttga gaataacctg
     1081 ctgggtggta atgaagacac tcagaccaag ttcgtgcagg agcatggcaa agatattgcg
     1141 tcctgctcga ctaaccctgg cggggctgaa tgtaaacgcg gtgaggctgt aaacaaagcg
     1201 attgcagcag cgcttgttgg gggggctaca ggtggggtag tgattgctct gactccggaa
     1261 gcaattgcag ccgcccaggc agcagtcagt gcctgtggtg cgaatactgt gctgtgtgca
     1321 aatgaagtta gtatctgggt tgcggaaatg gtcgctggcg atgctcttcc agcgggcctg
     1381 acagtagcgg cggttggtaa aatgtctgcc agcgagttgt cagagttgaa ggctctaatg
     1441 gctgttgaaa aacagacagg tcagaaaatc agtgcggagt cacttgaaaa gctgatgcta
     1501 gactcaggtg gtaaagggaa ctggagcaaa gagctaaata acccacagcc gaataaagtt
     1561 tatcatgtag atggtgataa aatttataaa actgatgctc aggcaaggcc aagccaggtt
     1621 gaagcaactc ttagtcctaa tgtaaaggat agaaatggct atcaacaatg taaagcaggt
     1681 aagtgtggcc ttgatggaga tgagggtggt catttaatag ccagtatttt taatggtcct
     1741 ggtgaaaaat tgaaccttgt tccaatggat ggaaatctga acaaaggagc ctggaagcag
     1801 atggaaaata cttgggcaaa agcattgggt gaaggaaaag aggttaaggt caacattcag
     1861 ccatcgtata ttggggatag tgtcagacct gctaaattta aagtagtata ttcgatagat
     1921 ggaggtcgcc cgattgaaaa agtatttaac aatgcacccg gaggtaaata atgcgttctg
     1981 atcaggatat ttataatgac attggttcgg tgttgctatc gatcactcct agtgatgcag
     2041 tcaaaatcat tctgtgtgca aagttagaac cggaaagcga ctgtggtgag tttacttatg
     2101 actatgtaga taaacagggc aatcaacatt ggatcactga aaccgcagat gccagtgaaa
     2161 gacttttaga tcttttggtg gaactaagaa atttgtttgt ggataacttt aaatcacaag
     2221 aaaagccatt ctggcatggt tgcgaagtca ctgtgaatat tgaaacactc aaaataaaca
     2281 tcgactttaa atatgagaat taatggtaat tccgcccttt ccggctcact tcgtcggcca
     2341 aattagtgtg atattctttc cagaaatgct gttgtatcca cgacataagg cgatgattga
     2401 tggagcagtc tctcaatttt gaaatgttgc gcagccagtg gccggaactg gctgaactcg
     2461 cgtgtatggc ggaacgctat gttcactccg atccggaaag ctgtctggtc aagctgcgca
     2521 actacaccga attgatggtg cgctggttgt atcgtcagga gcggttgccg gaaggtatta
     2581 aggctaatct ttacgattta atgaatgctg atgtctttac cagcatgatg ccggaagcca
     2641 tcatcatgaa aatggatgcg ctgcgtatcc atggcaaccg tgccgcgcac ggcggacgta
     2701 tcaaagctaa agatacttac tggctgctca aagaagcgta tttgttggga atttggctgt
     2761 atgttcgcta cgcccacggt aatgttgatg actgccccaa atttacactc cctctattaa
     2821 cacaatcttc aggtcgtgca gatgaaaaac gtctggaaga tgcaatcagg gctcaggatg
     2881 aaagccgtga gcgcgaactg gcgctgcaac gcgcactaca gcaagaacag gaaaaggccg
     2941 aacacctcac tcaacgcctg aatgaagcca gagcgcgtaa ccagcatgtt gccgatattc
     3001 tctctattga tgaagcggaa acccgccgtc gcctgattga ctctcgcctg cttgctgctg
     3061 actggaatgt aggcgaagac cttaaaaata ccgatcaggt tacgcaggaa catcccgtta
     3121 aagaacaacc taccgcgacc ggagacggtt atgcggacta tgtcttgtgg gatgaggcac
     3181 acaaaccgct ggcggtggtg gaagcaaaaa aaaccagcgt caatgccgag caaggacgaa
     3241 ttcaggcccg gctgtatgca gactggctgg aaaaagaata cggccagcgt ccgatcatct
     3301 tctacaccaa cggctatgat atctggctgt gggatgacca taaaactcat ggttatcccc
     3361 cgcgtcgggt gtttggcttc tacagcaagg aaagcctgca atatcttatt caacagcgtg
     3421 aaacccgtct gccgctgaac agtgtggcgc acgtaaaaga taacgaaggt aaggccgttg
     3481 ccggacggtt gtaccagctt gaaaccattg cccgtgtcag cgagcggttt accaacaagt
     3541 accggcaatc gttaattgtt caggctacgg gtacaggcaa aactcgcgtg gcaattgcac
     3601 tgagcaaact gatgatagat gcccgctggg taaaacgcgt cttgtttctt tgtgaccgca
     3661 aagaattacg taaacaggcg gcgaatgcct ttaatcaatt caccaatgag ccgttgtatg
     3721 ttgtcggtaa atcgaaaaaa gcagacaggc agaacgccag gatctacatc gctacctatc
     3781 ctggcatgat gaaaatcatg gaccatttcg atgtcggtta tttcgatttg atcatcgccg
     3841 atgaatccca ccgctctatc tacaacgtct acggcgatct gtttaagtat tttgatgccc
     3901 tccagattgg cctgacggcg acgccgattg atatggtgag taaaaccact ttcggcctgt
     3961 ttggttgtga aggacgtatt cctaccgcca actacagcct ggaagatgcc atcgccgata
     4021 acaatctggt tccctatgag gttgtcacgt acaccacaga attcctgcgc gaaggcatca
     4081 aacgggagaa attaagcgac gcacaaatcc gcgagctgga agaacaaggc atggatccca
     4141 atacgctgga gtttgatggc aaggcgctgg atgaggcgat ctacaacaaa gacaccaacc
     4201 gctatatcct gcgtaacctg atggaaaatg gcctgaaaga tcgggacggc cagttgccag
     4261 gtaaaaccat cattttcgcc cgtaaccaca aacacgcgct gctgttgaat gaattattcg
     4321 acgatatgta cccgcagttt gccgggcgct tctgccaggt catcgacaac tacgatcccc
     4381 gcgccgagca attgattgat gattttaaag ggctggatga aagcaccaac aaagagctga
     4441 ccatcgctat ctccgttgac atgctcgata ccggtattga tgtcccggaa attgtgaatc
     4501 tggtctttgc caaaccggtc aaatcaaaag tgaagttctg gcagatgatt ggccgtggta
     4561 cgcgcctctg tcctgggttg tacggttacg acgataacgg caaaccgctg gataaacaga
     4621 aattccgcat tttcgatcac tggggcaatt ttgagtatca cgaactgcat accgaagagg
     4681 ccgaagtcac agcgacaaaa tcgcttgcac aaaaacgctt cgaagcgtgg gttatgctgg
     4741 gggccgctgc gcagcgtaaa ttcgacaaac aggcggtgga tttagttgct catcagcttc
     4801 gcgagcaaat caacgcactg gatgaaaagt cgattgccgt acaggaaaag tggcagcaaa
     4861 aagcgcagta cagtgatgaa aaggtgctgc gccagctttc tccgaaaaca cagcaggatc
     4921 tgctatctgt tctggccccg ctgatgcagt ggctggacgt gcgcgggcaa agcgatgcca
     4981 tgcgctttga tatggatatt ctggcggcgc aaactgcccg ttataccaac ccggaagagc
     5041 tggatgtgct ctggccggtc atcgtcgaaa aagtggagcg cctgccgccg catttggcgc
     5101 aggtccaaca gcaaggaccg cggatcaatc agcttcgtga tttaggctgg tggaagcagg
     5161 ccagcctgga agagctggaa gatatccgca ttcatctgcg cggcatcatg cacctgatgg
     5221 aaaaagacgc gacgccgaaa tttggttcga tacaggtgga tattaccgaa gatgcgaacc
     5281 tgattcagac ggaaacccgc aaaaccaaca tccgctcgat tgacttcaaa ctctatcgcc
     5341 agcaggtgca gggagcgctg gagccgctgt tccagcaaaa tccggtgctg aagaaaatcc
     5401 gcaacggcga gcctgtgacg caaagcgagc tggatgaact ggctaaactg gtgctgatcc
     5461 agaaccctaa cgttgatatc cgggcactga aagagttcta cccgcaggcg accgccagcc
     5521 tggataaact attgcgtacc atcatcggga tggacagcga cgcggtggaa gtgcgctttg
     5581 ctcagttcgc cgctgataac agcctgacca gccagcaatt gcgcttcctg tcattgctga
     5641 aaaaccacat tcgcgattac ggcaccattg aaatgcggca gctctttgaa cagcctttta
     5701 cgcatatcca caacgaaggc gttaccggtg tgttccctga tatagagcag atcgcccgcc
     5761 tacaaaagat agtcgaagag ctgggtgttg tgaccgacgc agcaacggta taatacgcac
     5821 atgacaagcc ccggcagcgg ggcttttgta tttaaaacag gatataaaag ctaacaatga
     5881 tcactggtga cttaaaaagt aaaatcgacg gcctgtggga agatttctgg gtgggtggta
     5941 tcaccaaccc gctgaccgta atcgaacaga tcacctatct gatgtattcc cggatgctgg
     6001 atacccagga acaacgcgac gagaagcgta aacaaatcgc gggtattgat tttaaaccgc
     6061 gttttgcgcc agaacagcag gagttccgtt tcagtcacta tagcaatctt ggctcggatg
     6121 agatgatgga agtggtgcgc gatggtgtat tccagcattt tcgtcagctc ggccaggccg
     6181 atgcgtcgaa ggtaacgctg ctgggcaact ttatgaaaga tgctcgtctg gagattgtta
     6241 agccgtcatt gctgaccaaa gcggttgagg tgatcaaaaa cctgccattg gatcgcggcg
     6301 acaccaaagg cgacctttac gaatacctgt taagcaagct gaccactgct ggaatcaacg
     6361 gccagttccg cacgccgcgc cacattatcc gaacaatggt tgaaatgatg gagccgaacc
     6421 cggcccgcgg cgagacgatt tgcgatccgg cctgtggcac cggtggtttt ctggcaacca
     6481 gctatgaata tctgctggag aagtacagct cgctggaatc cattcatact gagattggca
     6541 ccaacgaacg tggcgagctg gaagagcaaa aaatctttac cggcgatctg ctgacaccgt
     6601 ggcgtaacca tgtggataac aacatgttcc acggttatga ttttgatacc actatgctgc
     6661 gtatcgccgc tatgaacctg attatgcacg gcgtggaagc gcctgatatc cactatcagg
     6721 acaccatgag ccagagcttc agcactaact tccctcaggc aagtaaaaac gctttcaacc
     6781 tgattctggc gaacccgccg tttaccggct cactggacga ggaagatatc gactccacgc
     6841 tgtcggcaat ggtgaaaacc aaaaaaaccg aactgctgtt cctggcgcgt attctgcaaa
     6901 tgctgaaagt gggcgggcgc agtgccacta tcgtgccgca gggcgtgctg tttggctcca
     6961 gtaaggcgca ccagtcactt cgcaaaacgc tggtggaaga taaccaactg gaagcggtga
     7021 tcaatctgcc ttccggcgta tttaaacctt acgctggcgt ggcgacggcg atcctgatct
     7081 ttaccaaagg cggtcagacg gacgaggtct ggttctacga tctgcaaaat gacggctaca
     7141 gcctggatga caagcgcaac ccgataaaag acaacgatct gccgcatctg ctggcaagct
     7201 ggaagtatta ccgtacttta cgcgggctac cggttgataa ctttatgggt gagaagtcag
     7261 cctcgttgct taaacagcag cacccggaag ggattgatgt tggcgttgat tttaaagatc
     7321 gcacgcaggc ggcgtttgta gttccaaaag aagatattgc tgcgcagaaa tacgatctct
     7381 ctattaatcg ttataaagaa gtggtgtatc aggcggagga atatgaagat ccgaaagtga
     7441 tactgaaacg attaaaagat ttggaaaaag agattctggc tgatctggat gaactggagg
     7501 ggatgctgtg agttggccga aggtgaagtt gggggatgtg gcacctgcaa ttccagttaa
     7561 aaatgttaat ttggacgaag atgaactggt ttggcagtta aatcttgatc aaattgaatc
     7621 caatacagga aaaatcgttg ataaagttaa acaaccttta tcaagtgcag gctcgtctac
     7681 tcattttttc aataatgaac atgttctgta ttcaaagcta cgtccttatt taaataaagt
     7741 agttgttccg gatgagttgg gtatcgcaac tactgaacta gtaccaatga agccggacca
     7801 gaagaggtta aataaagaat atttagccta ctacctgcgt tcaaagccat ttgtcgaatg
     7861 ggtatctacc caagtctctg gtgcaaaaat gcctagagtt gtaatgagta ttttctggca
     7921 acatgaaatg cctcttcctc cattatatga gcaagagagg attgtttcag ttctcgacaa
     7981 atctaataga ttacgccaaa aacgtgaaca agctatcaaa ctggctgatg attttttgcg
     8041 tgctacgttt ttggagatgt ttggcgatcc cattgaaaat ccgaagggat ggaatgtcaa
     8101 acctctggca gatcagattg ttcatgccaa taacggtatc tcacgacgta gaaaagaaga
     8161 tactaatgag ggagatattg tacttagact acaagatgta cattattcag ggattgcatt
     8221 tgataaagaa ctaaacagga taaaactagt cgataaagaa aaacagattg ctcgtgttga
     8281 gcatgatgat cttcttttta ttcgcgttaa tggaaatccg aactatgttg gccgtacggc
     8341 tgtattcaag tcatatattg aacctgtgta tcataacgat catttaatca gaattaaatt
     8401 agataacgaa tatcagtctg attttttatg ttatttaatt aactcacctt ttagtagaaa
     8461 attaatcgca cagcagatta aaacctctgc cggacaacac acaattagtc aagatggtat
     8521 attaaaactt atgttttatc gccctcctat t
//
LOCUS       DAZFFG010000163         8521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFFG010000163 DAZFFG010000000
VERSION     DAZFFG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.164"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..397)
                     /locus_tag="ACHJ7J_004469"
     CDS             complement(<1..397)
                     /locus_tag="ACHJ7J_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002607.1"
                     /translation="MHTFANLMYDVYQSFGLFSKGDRQGEIRASATFSGHQRFFGNRD
                     DEKHQEQKRYDEIIRIIDVEKVFSTVQRREIFYKYEQLYNALMARPSFTKLSRKQVQK
                     QYALHIIPRLIALDIYKTYKIQNKNSFYHH"
     gene            498..722
                     /locus_tag="ACHJ7J_004470"
     CDS             498..722
                     /locus_tag="ACHJ7J_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002608.1"
                     /translation="MSSFQTTQPEPQQRILRMRELTKIVGLSRSTIYEKLNPESRYYD
                     ETFPKPVRLGAASVGWRSTSVDEWIASRSV"
     gene            747..2273
                     /locus_tag="ACHJ7J_004471"
     CDS             747..2273
                     /locus_tag="ACHJ7J_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5002609.1"
                     /translation="MKLTYKKTENHDVNLSARAILYFFQKVIFCIYGKTRAAVELIFA
                     TLQGSMALACQDMYDVEIDDGVIVPVSQFHIILARSGSRKTTVYRLIMAQIHQIEREL
                     ADVFSVRLKEYERQYVLWDEECKSLKKLLQKAVRQKDGVDKARNELEECLKNQPIKPV
                     RVRLTVTDPTAEALLKELGEYPSLGITSDEGSALHESIMRRKTAVPNTLWCGDDYRVS
                     RVSTGCYDIKDPRLGMLLMTQPEPHDSTLKSLGNTVRATGVYARTLTMDLTLLSRLID
                     IDELVSGDESDLETFYSIQKKHLLAGIERREKNHPRICMTFSPDAKKRLHDVGRYVKH
                     LMQPGKKLYHHSDWAARYIEHTARNAAVMQLFITPDSTVITLETLEAALLISEWYLNH
                     FIVKMDAVRGPSHSERVIWWLENHLVKNGSYDFLKRDMMQGLHRSLRKKADLEPVLEQ
                     LAEEGKVQLWEDESGTHYVKYLASEMTPSELDAKHNMLSGKPAYHCVGSVFSNPPLKE
                     "
     gene            complement(2364..3572)
                     /locus_tag="ACHJ7J_004472"
                     /pseudo
     CDS             complement(2364..3572)
                     /locus_tag="ACHJ7J_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3807..5220
                     /locus_tag="ACHJ7J_004473"
                     /pseudo
     CDS             3807..5220
                     /locus_tag="ACHJ7J_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            5605..6198
                     /locus_tag="ACHJ7J_004474"
     CDS             5605..6198
                     /locus_tag="ACHJ7J_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5002610.1"
                     /translation="MKPYNANPNYVMNGQILEDINAHMEAMFQRFAKLLPFRIDFAYR
                     KNTASFGHVCKHTMCTEILHLLTETENAMAGYYWVMEYTVKKGLHIHLLGYLNGQYHQ
                     NPYQLSRLMGDVWERATEGDGYHHLCRAKENYPVRIDQVIHYADTAAMNDLRYAISYL
                     AKVKQKEHGAIVGRSTVPEKSNRGRPRQRGNGVTRIC"
     gene            6557..6958
                     /locus_tag="ACHJ7J_004475"
                     /pseudo
     CDS             6557..6958
                     /locus_tag="ACHJ7J_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            6967..7363
                     /locus_tag="ACHJ7J_004476"
                     /pseudo
     CDS             6967..7363
                     /locus_tag="ACHJ7J_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            complement(7589..7839)
                     /locus_tag="ACHJ7J_004477"
                     /pseudo
     CDS             complement(7589..7839)
                     /locus_tag="ACHJ7J_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7840..8520)
                     /gene="ydeO"
                     /locus_tag="ACHJ7J_004478"
                     /pseudo
     CDS             complement(7840..8520)
                     /gene="ydeO"
                     /locus_tag="ACHJ7J_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YdeO"
BASE COUNT         2255 a         1915 c         2063 g         2288 t
ORIGIN      
        1 tatgatgata aaaactgttt ttattttgaa ttttatacgt tttatatatg tcaagtgcga
       61 ttagtctggg gatgatatgc agagcatact gcttttgtac ttgttttcga ctcagtttgg
      121 taaagctcgg tcgagccatc agcgcattat aaagttgctc atatttataa aagatttccc
      181 ttcgttgaac tgtactgaac accttttcta catcaataat tcttatgatt tcatcatagc
      241 gtttttgttc ctgatgtttt tcatcatcac gattaccaaa aaatcgctga tgacctgaaa
      301 aggttgcaga tgcccgtatc tcgccttgcc ggtccccttt actgaataag ccaaaggatt
      361 ggtaaacatc atacatcaga ttggcaaacg tatgcatagt cactccctgc agttggtttc
      421 gtttctttta cggggacatt attccaaatc ctgtgccaaa gttcggccac atccggacat
      481 tcacaggaga atcgataatg tcatcttttc aaaccaccca accagagcca cagcaacgta
      541 ttctgcgtat gagagaactg acgaaaatag tcggtctgtc ccggtcaact atctatgaaa
      601 agctaaaccc tgaatccagg tattacgacg aaacgtttcc aaagcccgtc agactcggtg
      661 ctgcctccgt tggatggcgt tccacttccg ttgatgagtg gattgcatcc agaagtgttt
      721 aattttttct aaagagagat tatgcaatga aactcacata taaaaaaact gaaaatcatg
      781 atgttaatct gagcgccaga gcgattttgt atttttttca aaaagttata ttttgtatat
      841 atggcaaaac aagagcagcg gtggagctta tctttgcgac gcttcagggg agcatggcct
      901 tggcctgtca ggatatgtat gatgttgaaa ttgatgatgg cgttatcgtt cctgtgtcac
      961 aatttcacat aatactggcg agatcaggaa gcagaaaaac gacagtatac aggctcatca
     1021 tggcgcaaat tcatcaaatt gagagagagc tggctgatgt gttctcggtt aggctgaagg
     1081 agtatgagcg gcaatacgta ctatgggatg aagaatgtaa gagtcttaag aaattgcttc
     1141 agaaagcagt gcgtcagaaa gatggagtgg ataaagccag aaacgaactg gaggaatgtc
     1201 ttaaaaatca gcccattaaa cccgtgagag tgcgtctcac ggtaacggat cctacggctg
     1261 aagcactttt gaaagagcta ggggaatatc ccagtctggg tatcacgtct gacgagggaa
     1321 gtgctctgca tgaaagcatt atgcgccgta agacagcagt tcctaatacg ttgtggtgtg
     1381 gtgatgatta tcgggtcagc cgtgtgtcaa cgggctgtta cgatattaag gatccacggc
     1441 ttggtatgct gctgatgacc caaccggaac cacacgatag cacactgaaa tcgctgggca
     1501 atacagtacg ggctacggga gtatatgcgc ggacgttaac catggatctt acgttactca
     1561 gcagactaat tgatattgat gagctggtat ctggcgatga gtccgacctg gaaacgtttt
     1621 attctattca gaaaaaacat ttacttgcag ggattgaacg cagggaaaaa aatcaccccc
     1681 gtatctgtat gactttttca cctgatgcaa agaaacggct acatgatgtt ggcaggtacg
     1741 taaaacatct gatgcaacca gggaaaaagt tgtaccatca cagcgactgg gcagcacgat
     1801 acattgaaca tacagcaagg aatgccgctg tgatgcagtt gtttattact cctgactcaa
     1861 ctgttattac gctcgaaacg ttggaagcgg cgttgttaat atcagaatgg tatcttaacc
     1921 attttattgt aaaaatggat gctgtcagag ggccttcgca ttctgaaaga gttatctggt
     1981 ggctcgaaaa ccatcttgtt aaaaatggat cttatgattt tttaaagcgt gacatgatgc
     2041 agggcttgca tcgttcactc agaaaaaaag ctgatcttga gcctgttctg gaacagcttg
     2101 ctgaggaagg caaagttcag ttatgggaag atgaaagtgg aacacattat gtcaaatatc
     2161 ttgcttccga aatgacgcct tcagagctgg acgcaaaaca caacatgctg tcaggaaaac
     2221 cggcatatca ctgtgtcgga agtgtattta gcaacccccc tctgaaagaa taactttatg
     2281 gtgattttta tttatatatg cgttaatttt ttattaactt atggcaggga gcctgtcagt
     2341 aattctgtgt aactgccaat gtattaaagg tgatcgctca ggcggtcacc gaactcgata
     2401 ataaaacggc tcattgctaa ccgccagtta tggatcggca tactctattt tttcgacgca
     2461 tctttggtcg ccagataaat aacctttcgt accgagtcat ccgtcgggaa cactttgcgt
     2521 ttcttaatcg cagctcggat catactgtta agcgattcga ttgcgttcgt ggtgtagatg
     2581 gccttgcgga tatccggcgg ataactgaag agcgtattga ggttttccca gtgcgcatac
     2641 cagcttttgc tgatttgcgg atatttatcg tcccagactt ctgcgaacgc atcgagcgcc
     2701 atcagtgccg cctcttcggt cgggacctga tagacagtct tcaagccgct ggtgacggct
     2761 ttgtagtcct tcctggacac gtatttcagg ctgttacgcg ccacatggat gctgcacagc
     2821 tggatatggg gctgcgggaa gacgctgttt atcgcatccg ggaagccctt aagaccgtcc
     2881 acacaggcta taagaatgtc ctggaggcca cggtttttca gctccgtcag cacactcagc
     2941 cagaactttg cgccttcgtt ttcggtcagc cacatgccca gcaactcttt ctggccttcg
     3001 gtattgatcc ccagcgccag gaagacggcc ttgtttatca cactcccatt ctgacggact
     3061 tttacgacga tacagtccat gtaaacaatg ggatacagcg catccagctg tcggttttgc
     3121 cactcagtaa cctgttcttt taccgcgtcg gtgaccttat atatcagcgt gggtgacaca
     3181 tcttcgtcgg acatttcttt gaaggtggcg acgatttctc gggtagtcat gcctttggcg
     3241 tagagggata aaatgttgct gtccatctga gtaatgcgcg tctggtgctt cttaatcagc
     3301 tgcggctcga aggtgttttc acggtcacgc ggcgtgttca gttcgatctc gctatcatcg
     3361 cataacaccg tttttgacga gtagccattg cgggtgtttg aacctgattt tggtgcattt
     3421 ttctcatgtc cgaggtggtc agtcagctcc gcattgagcg ctgtttcgac ggctaatttc
     3481 gtcagcatac ggaaaaactg attgagctcg gcttcggttt taaggtcctt agccagttca
     3541 gccgccagtg ctttgagttt cttttcgtcc ataatttgcc tgtctccgtt attggagtga
     3601 acatatcaaa aacaggcaat tacacaattt taattacagt ctcatggcag gctgcggcga
     3661 ccatccctcc agccatcggt tcgtctgaga ataatgtgca actgtgcacg ctacacccgg
     3721 ggacaacggc tgatgaagct tattccccat cgccgggtaa ttaagggcgc gagcgcaatc
     3781 caattttttg catgccaata ttcaacacag ggcagacgct atggcttgta ttttccgccc
     3841 gccactggta ctgccgtaac ccagcttgtt cacgtaaaat ttttgccgag tcgcttactc
     3901 ccttcgctgg ttcacaccag cagtcttcac aggcgttaca aaatttacaa cgtcaactgg
     3961 gattaatagc tggaggtgag gctggaaaac gggcagcaac ggcagtgggt ctctgttgca
     4021 gtgcagatac tcttcttcgc agagttatca ataccccgga gacgaaactg tcaggctcgc
     4081 cccatgtcgg tattgatgag tgggcgtggc atcggggcca ccgttacggt acgttaatag
     4141 tcaatcttga tactcactgt cctgcttccc gaccgcgatc agcgtacgct ggcgacctgg
     4201 ttcagaaaat atccggaaat acaggttgtc tcgcgtgatc gcagtggaac ctatgcaaca
     4261 gcagcacgtg aaggtgcacc tcaggtcaga caggtggctg atcgatagca tctgctaaaa
     4321 aatattggcg atgcgcttga acgattgatg tacagacata ggcatctgat acgtcttgtt
     4381 gccagtgagt tgccaccaaa gaaatcacct gagccagaac tgtctgtgcc tgcagcatcg
     4441 ctccgtcgtc cggaacgcct taaacagcaa atccgcaaaa aacggcatca gcgttggaca
     4501 gaggttatgg ccctgcataa caagggatgt agtttcaggg aaatatcccg tattacaggc
     4561 ctgtcgcgag tgacagtcag tcgctgggcg cgttcaggaa cattccctga aatgtcaacc
     4621 cggcctccaa agcgaggact tctggaccct tggagggagt ggttaaaaga gcaatgagaa
     4681 agtggtaatt ataatgccag ccggatatgg cgggaaatgg tgacccgggg ggttacaggc
     4741 agtgaaaaca tcgtcaggga tgctgttgcc aaatggtgta aaggctggat cccgctggtg
     4801 actactgctg ccagacttct ttcagtgtac cgggtaagcc gctggttgat gccctggaga
     4861 ataatcaggg gtgaagaaaa ctatgcttcc cgatttatta gtctgatgtg tgaaaaagaa
     4921 ccggagctga aaatagcgca tcaactggca ctagagttct accgtattct gaaaacccaa
     4981 actaaatcac ggctaagcag ctggttcact cgagtccacg aaatcgattc agcagaactt
     5041 cggcgcgtgg ctgcggtgat ggaagccgat gctgcggcta tatgtgaggc aatcagcagt
     5101 cgctggagta atggtgttgt cgaaggtcat gtaaatcgcc tgaagatgct caaacgccag
     5161 atgtatggcc aagctggatt tgaactactc aggcagaggg taatgagtcc attggcatga
     5221 aaatctccac ccaagttgcg gaagagccac ttttgcaccg ttcgtgacat tacgtgatgg
     5281 gatcaagaca gataaattaa ggttaattag tattatttgc agaagttata tcgtgacgta
     5341 gtgatagtta tgttgtctta tcctggtatg ctacttgctc tttcccgtgc tgtcagttct
     5401 gcctgctgag atcatacagg acagatccat cccccttatc tgataacaat ctgctctcct
     5461 catatcaatg gtatccgtca gtctcgccat gagtagtaca tgttgctggt tccctagggg
     5521 aagctacagg tgaatgtgag gtcaatcggc ctgtgtaaaa attacgtaac aacgtcttcc
     5581 tctgactcaa caagaggaag acaaatgaag ccttacaacg ccaatcctaa ctacgtaatg
     5641 aatggtcaga ttctggagga catcaacgcc cacatggaag cgatgtttca gcgtttcgct
     5701 aagctgttac ccttcagaat tgatttcgct taccggaaaa ataccgccag tttcgggcat
     5761 gtctgtaaac acaccatgtg tacagaaata ctacacctgt tgaccgaaac agaaaacgcg
     5821 atggccggtt attactgggt gatggaatac acagtgaaga aaggtctgca tatccatcta
     5881 ctcggttatc tgaacgggca gtatcaccag aacccttacc agctttcccg tttgatggga
     5941 gatgtctggg aacgggcaac agaaggagac ggataccacc acctgtgccg tgctaaagaa
     6001 aattatccgg tccgaatcga ccaggttatc cactatgcag ataccgcggc aatgaatgac
     6061 cttcggtatg ccatcagtta tctcgcgaaa gttaaacaga aagagcatgg cgctattgtg
     6121 gggcgaagta cggtaccgga gaaaagtaac agaggcaggc cacgacagcg cggtaacggt
     6181 gttactcgta tctgctgata agtggtttat aagaactata tcgcctttag tggaacgttc
     6241 atagcggtat gagcaggtaa atctccggtc cctgtacgtt acgacacgtt ggtatccgtc
     6301 agtgaccaga gctcagcagt ctggctgtat caacgtcccc cgaattcact gacgtggggc
     6361 cacgacgcag atgtcgatta ttttttacgt cagtgcccca tagtgtgaca accacgcaac
     6421 aataacgaca gactgcacag gcttacttta cctccttttg ataacacttc actaatccgc
     6481 cattcacacc ctgaccacgc tacccgtagc gggacggctg acgtctgcgt gtcgtctggc
     6541 ggtaaggaac tacactatat acgcaaaaaa ctttatcgct ttcaaaggga atgatcgtct
     6601 gattgatgcc cgtactgcac agaccgcacc ttgcgatcgc tacaccggcc atccttgcgc
     6661 tagtgcactc aaataccatc cggaatacga tactgaacgt cgctgatttg aacacaccgt
     6721 caaatggctg actgagcatg gtcacgagtg ccctcatgtc agaccggaac gcagtaaagt
     6781 acagttgatt aaacgcttac agcaattgat accgaatgcc ttacccgtgg tgcgtaaagc
     6841 cagctggcac tgcagacaat gtcgccacga ttattatggg gagcggtact gcatacactg
     6901 ccagaccgga ggatgcagta ttccccggac aactcaggag gaaatatatg aattctgaac
     6961 tgttatatga caaatgacag caaaggtcat tttgtaacag cgaaagaagc cgcgaagcac
     7021 agccgccagg acgttttgtg ctacgtgttc tgtggttatc cattaacact taagcggggc
     7081 aatgacggac aaccgccgtg atttgagcat gaccagatga ctgtcgctga gaaaatcctc
     7141 ctgcgatgca ccaggcctga cacggtagag aaagaggccc gtcgtttgca tctgcagggc
     7201 atgacggttc cggattatat ggtgaagatg agaaagtggt tttgtgtgat gtgtgacgaa
     7261 gattatgagg ctcagaagtg ctgtggtata cagcagagat ggagaacggc aggaggcacc
     7321 gccggaagat gccggggctg caaatccagt gcagaggctg taatggttgc tcccgggcgc
     7381 agtttgcggt gaggttgttc cgggtatggc ttgccagtac ggggacagta caacttaatt
     7441 ccagtgctgg cggcctttcc gttgattttg tgccaaaagc tccctgtgac tgacaggcag
     7501 cctcgtcatt ccattcgtat cgccagtaca cgagcgcttt cgccagttct gagtgtcgtg
     7561 gcagggtttt catcttttca cgcagtcagc tttccaagga tttcagcagt agtttcgctt
     7621 tctgctgaca ttcagcaaga cgttactccg ccggcattcc ctttatttcc gactcgttgg
     7681 tatacatcct accgacccct ttcagtgctt catccgtcag cgccgacggc gtgcggacat
     7741 gcacaccatg gttcttgttg cgggcatgaa cccagcgttc gcatccgtct gcagaatacc
     7801 gctgaagtct gcaagatggc tatgcgggtg gatacctttt tccttagaaa atctctttgg
     7861 actgttgccg aaataatttc ggaaagtata aataaaataa gatgtactgg cataaccaca
     7921 ttgttcggca attttattga ctgagccttc tatccgcatc aatttattag cgtgctgcat
     7981 tctggcatct aaaagaatct gtgagaatgt tgtttgctct tgttttagtt ttttcttcag
     8041 caagctttca ctcatgcaca ggcagttaca aatgtctttc agtttccatg aatgggataa
     8101 cttcatattg acaatgttcc tcacttttcc ggaaacggat aatacaccgt tattaagcaa
     8161 tgtaatgaaa cctttgcatt cggaaaaaat agataggcaa gataaaagca aaagttcaga
     8221 gaaatcatga tgattatttt cttccgagtt gagataaata atcatttcat taagcaatcc
     8281 agtcggtaca ttagaaacac gtaaaaactt tgtaggagca gacattgtct ctatctgaat
     8341 gttagtgtat tgtaggtatc ttttgatgat tttttcattg agtttgattt ctttgaaata
     8401 ccgttcataa ggtttaaaat ataaattaac agcactatct accatcagaa cctcaccgtc
     8461 agagaaggcg tagttgttat ctaaaatata tgcattaaat tcatgttgaa tgaatttaat
     8521 a
//
LOCUS       DAZFFG010000164         8512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFFG010000164 DAZFFG010000000
VERSION     DAZFFG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.165"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1222)
                     /locus_tag="ACHJ7J_004479"
     CDS             complement(<1..1222)
                     /locus_tag="ACHJ7J_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5002611.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISLADEWNSVNARLKQAS
                     QSSDDFQASQRELMAISQRTGTAFSDNASLFARSAASMREYGYSSEEVLKVTEAISTG
                     LKLSGASTAEASSVITQFSQALAQGVLRGEEFNSVNENGDRVIRALAAGMGVARKDLK
                     AMADNGKLTADKVVPALISQLGALRDEYAAMPDTVSSSATKVENAFMAWVGGANEASG
                     VTKTLSGVLNGIAGNIDTVATAAGALVAVGVARYFGNMASSAGSATAGLITAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVVAARGTERQAAAEAKLTAAQASLTRNIAART
                     AAQTTLNTVTSVGSR"
     gene            complement(1270..1548)
                     /locus_tag="ACHJ7J_004480"
     CDS             complement(1270..1548)
                     /locus_tag="ACHJ7J_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5002612.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRFSPLGDERADIRA
                     AQIVSAVYGAQGVKVPLNDALLQWEKEQTEGVSDPFAGLENALLIVSQ"
     gene            complement(1572..1943)
                     /locus_tag="ACHJ7J_004481"
     CDS             complement(1572..1943)
                     /locus_tag="ACHJ7J_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5002613.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            complement(1958..2662)
                     /locus_tag="ACHJ7J_004482"
     CDS             complement(1958..2662)
                     /locus_tag="ACHJ7J_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HFO5002614.1"
                     /translation="MSSNFERSQLTKIMISSAPVTAETLDSASYLGLSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHTWSVGTNGVVAATFSLRLKGKPVLSTEPLKVKVDLNSTLQVS
                     AGAKLEMVVEAAGGVPPYSYVWKKGSSPVSGQTAATFSKASAVSGDAGAYTCEISDSA
                     SPVNKVVSTSCTVTVS"
     gene            complement(2723..3067)
                     /locus_tag="ACHJ7J_004483"
     CDS             complement(2723..3067)
                     /locus_tag="ACHJ7J_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO5002615.1"
                     /translation="MRETTLYSLLSQLAGGQVYPYVVPLTEGKPAVSPPWLVFSVVSD
                     TASDVLDGQAESRITVQIDVWATVPDDADDIREQALDAVRQLVPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(3064..3513)
                     /locus_tag="ACHJ7J_004484"
     CDS             complement(3064..3513)
                     /locus_tag="ACHJ7J_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5002616.1"
                     /translation="MMDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEVMRDA
                     VVERAPERTGKLKKNVVVLTQRSKRRGEIISGVHIRGRNLRTGNSDNSMKASDPRNAF
                     YWRFVELGTVNMPAHPFIRPAFDTKEELAAQVAIQRMNQAIDEVLSK"
     gene            complement(3510..3848)
                     /locus_tag="ACHJ7J_004485"
     CDS             complement(3510..3848)
                     /locus_tag="ACHJ7J_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5002617.1"
                     /translation="MQAGRLRDRVIILNVTTARSPSGHPVETVTEGATVWAEVKGISG
                     REIISGGAETAQATVRVWMRFRSDVTATSRLKVLTGAFKGAILGIEGTPIPDARATRL
                     EILCSLKGNV"
     gene            complement(3857..4174)
                     /locus_tag="ACHJ7J_004486"
     CDS             complement(3857..4174)
                     /locus_tag="ACHJ7J_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5002618.1"
                     /translation="MAAIVEKLRAQCRIDTDDATDDELLILYFRAACRKAENFINRKL
                     YEETVPEGDPEGVLIADDVLLALMLLVGHWYENRENSSDVSKAPVPFGFSSLLEPYRF
                     IPL"
     gene            complement(4219..5445)
                     /locus_tag="ACHJ7J_004487"
     CDS             complement(4219..5445)
                     /locus_tag="ACHJ7J_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5002619.1"
                     /translation="MMADIKDVEQVAQELQAKFDDFKEKNDKRIDAIEQEKGKLAGEV
                     ETLNGKLAELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLREL
                     ERKAMQVGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLGGSDYKKLVNLGGT
                     TSGWVGETDARPETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALE
                     FAEQEEIAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKL
                     IYTLRKVHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIDLGQPSSLAGYGIVENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLMKIGAATRQKAAA"
     gene            complement(5456..6106)
                     /locus_tag="ACHJ7J_004488"
     CDS             complement(5456..6106)
                     /locus_tag="ACHJ7J_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5002620.1"
                     /translation="MQTKQRLDIPLNLKSVSDSGEFEGYGSVFGVKDSHDDVVVPGAF
                     TTTLQKWSEKKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLVDDDPLAKRAHA
                     HMKAGSLTGLSIGYILKDWEYDREKGVFLLKEIDLWEVSLVTFPSNDEACISDVKNAL
                     ARGEIPDQKIIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVDAALNALKNLKF"
     gene            complement(6084..7325)
                     /locus_tag="ACHJ7J_004489"
     CDS             complement(6084..7325)
                     /locus_tag="ACHJ7J_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5002621.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELAEAIGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTS
                     PSAGDDNGKKKESGDADKTAS"
     gene            complement(7325..7507)
                     /locus_tag="ACHJ7J_004490"
     CDS             complement(7325..7507)
                     /locus_tag="ACHJ7J_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002622.1"
                     /translation="MFLIILTPLVGVLGVLLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(7519..8253)
                     /locus_tag="ACHJ7J_004491"
                     /pseudo
     CDS             complement(7519..8253)
                     /locus_tag="ACHJ7J_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
BASE COUNT         2039 a         2505 c         1908 g         2060 t
ORIGIN      
        1 aacggctccc cactgacgtg acagtattca gcgttgtctg tgccgctgtt ctggccgcaa
       61 tattacgggt aagtgacgcc tgagcagctg tcagcttcgc ttctgctgcg gcctgccttt
      121 cggtaccgcg agcagcaaca accgcctgtt gcgcacgata aaccgccgca cgcgccctgg
      181 cggttgctat ctgtgtcccc cggagttgcg cttccgcaag agccacttcg tttctggctg
      241 cagtaattaa tccggcagtt gcagatccag cagaagacgc catattgcca aaatatcggg
      301 ctaccccgac ggcaacaaga gcaccggcag cggttgccac tgtgtcaata ttgcctgcaa
      361 taccattcag cacaccggag agcgtcttcg tcactccgct tgcctcgttc gcaccaccaa
      421 cccaggccat aaaggcgttt tcaactttgg ttgcagagga tgaaaccgta tcaggcattg
      481 ccgcatattc atcacgcaac gccccaagct gactaatcag tgcaggaaca accttatcgg
      541 cggtcagttt tccgttatcc gccatggcct tcagatcctt acgggcaaca cccattcccg
      601 cagccagcgc acgaataaca cgatcgccgt tctcattcac agagttaaat tcttcaccgc
      661 gcagcactcc ctgcgccagt gcctgactga actgcgtgat caccgaactg gcttctgctg
      721 tactggcacc ggataatttc aggcccgtgg agatcgcctc ggtgactttc agtacctcct
      781 cagaactgta accatactcc cgcatggaag ctgcagaacg ggcaaaaagg ctggcgttat
      841 cagaaaacgc cgtccccgtt ctctggctga tcgccattaa ttcacgctgt gatgcctgaa
      901 aatcatcact ggactgtgag gcctgcttca gacgggcatt tactgaattc cactcatcgg
      961 cgagagaaat aagatgaccg gtagcaaaag ccccggcaaa tgcccccgcc atattcagtg
     1021 ccgaagattt agctgtattt atctgatccg tcacttctgc cagtgcacgc cgcatttcac
     1081 gggatgcagc agcggactgc cggcctccgt tctgcatggt acggtagtaa tcctgcccca
     1141 tacgcgaagc ccgggagatc tctgactgga atgaccggga atttgccgag attttaataa
     1201 tcagttcacg taatgtcgcc acactcattc tccggacgaa aaaaaaaccg ccgaagcggt
     1261 tatgttgact cactgagaca ctattaaaag cgcgttttcc agtccggcaa atggatctga
     1321 gacgccttct gtctgctcct tctcccactg aagaagcgca tcattcagtg gtactttgac
     1381 accctgcgca ccgtaaaccg cagaaactat ctgggcagcc cggatatcag cccgttcgtc
     1441 ccccagcggg ctgaacctgt caaattctgc ccacatcatg atttctgatg cggacatttc
     1501 ccgacgtaac tctgacaatg tgcgccccat cctgagcgcc agcatcatca gaaaacgcat
     1561 ccccggaagt gctacttttt tttaacctcg ccggcatcac tgatcagttc cagagactgc
     1621 cgaagaagcc gcgcatgcac cgggccatac acggcaatca cctgttcacg atcatcctct
     1681 gaaaatacag gttgcagtcc ggtatcacac agaacatcaa tgaacagttc aacatctgct
     1741 tccagatttc ggcgggcgcg ctccgcaacg gataacggtg tctcatcatc ttttgcttta
     1801 acgatctcct gccagcgcaa ccaggcttct gcagaaggtt cccgtaatac aaccgttgcc
     1861 ccttcccatt caggcacatc aacagtttta tggcgaaacc ccgacatcgt tgccagtgcc
     1921 agattacgga tatttttagt catcacatct atcctcatta actgacggta acagtgcagg
     1981 aagtggagac caccttgtta acagggcttg ctgaatcaga aatctcgcag gtatacgcac
     2041 cggcatcacc ggatactgct gatgccttac tgaacgttgc cgccgtctgt ccggaaacag
     2101 gagaactacc tttcttccag acataagaat aaggcggcac accaccggca gcctcaacca
     2161 ccatttcgag tttcgctccg gcagaaacct gcagcgtgct gtttaaatcg accttcactt
     2221 tcagcggctc tgtcgacagc acaggtttac ctttcaggcg cagggaaaac gttgcagcca
     2281 caacaccatt ggttcctaca gaccaggtat gctgacgcac ctctgccata aaggtaaatc
     2341 cgtttcctga cggaaaaata actttaaagc catacgtggt gtcattgtca taggcactgc
     2401 gcaacgcgtt ctgggcagca ttgaggtaaa agttgcctga catggaaatc tctgacgcgg
     2461 caccaagacc gttaatattt tcctgctcaa cagaacacag cgtggtgaca tcgatatcct
     2521 gcttttgtcc tgcggtaaac tgcacctctt tgattgtaca gctcaggcca agatagctgg
     2581 cagaatccag ggtttctgct gttaccggtg cagacgaaat cataattttc gtcagttgcg
     2641 aacgctcaaa attagaggac atactcgtct cctgaaaata aaaaaacccg ccagcggcgg
     2701 gtgggtaaaa tcattaacga cctcaggcta ttacctgaaa ttcaagcgtg gctctgctca
     2761 gacgggaatc aggatcataa ccctgagttt tagaaataac ggagggtaca agttgcctta
     2821 ccgcatcaag cgcctgttct cggatatcat ctgcgtcatc aggcaccgtt gcccagacat
     2881 cgatctgcac ggtaattctg gattcagcct gaccatcaag cacatcagac gccgtgtcag
     2941 acaccacaga aaacaccagc cacggcggag ataccgcagg ctttccctcc gtcagcggga
     3001 ccacataagg ataaacctgt cctccggcca gttgagacag cagggaatac agtgtggtct
     3061 ctctcattta cttaatacct catcaatagc ctgattcatt cgctgtatgg caacctgcgc
     3121 cgccagttct tctttcgtat caaacgccgg acgaatgaac gggtgagcag gcatgtttac
     3181 cgttcccagt tcgacaaagc gccagtaaaa cgcatttcgg ggatcgctgg ctttcatgct
     3241 gttatcactg tttccggttc gcagatttcg tccgcgaatg tggacacccg agataatttc
     3301 cccccgacgc tttgaacgct gagtgagaac aaccacattt ttcttcagtt tcccggttcg
     3361 ctccggcgca cgttcaacaa ctgcatcccg cataacttca gcaccggcac gggtagcatc
     3421 gcgcagaacc ttattgtttt ctgccctgct gagcgtctcc agatcccgtg caatatccgc
     3481 caggccggaa aaatcaagac tgaaatccat cacacattcc ccttcaggct gcagagtatt
     3541 tcaagccggg tagcgcgtgc atccggtatt ggtgtacctt ctatacccag aatggcccct
     3601 ttaaatgcac cggtcagcac tttcagacgt gaagtcgctg tcacatcgct ccggaatctc
     3661 atccagactc tgaccgtagc ctgagcggtt tctgctccgc ctgagattat ctccctcccg
     3721 ctgataccct taacttctgc ccatacggta gctccctccg tcaccgtctc caccggatgc
     3781 cctgacggag agcgggcggt ggtgacattc agaataatta cgcgatcacg taatctgccc
     3841 gcctgcatgc ctcctcctac aaaggaataa aacgataagg ctccagcaga gaagaaaaac
     3901 caaacgggac tggtgccttg ctgacatctg aggaattttc ccggttttcg taccagtgcc
     3961 cgaccagcaa cataagcgcc agcaaaacat catcagctat aagcacccct tctggatccc
     4021 cctccggcac cgtctcctca taaagcttac ggttgataaa attttctgcc ttgcggcagg
     4081 cagcccggaa atacagtatc agtaactcat catcagttgc atcatctgta tcaatacggc
     4141 actgcgccct gagtttttcc actattgctg ccatcagaaa ctcctgcccg caacactgtg
     4201 cgggcataaa aaactgcatt acgcagcggc tttctggcgg gttgcagcac cgatcttcat
     4261 cagcttaatc gcctgagaat ccaccagcat accgccggtt cgtttggtgg tataaaagcc
     4321 cacaaacggt ttgttggtgt aaggatcgcg caggatacgg gtaccgatac gatcaacgat
     4381 ggtatagccg cgtttgaagt taccaaacgc aatggctttt gcatcggcgg caatatccgg
     4441 catctgctca ttttcaacga tgccataccc tgccagagaa gaaggctgac ccagatcaat
     4501 acccggacgc cacagataat ttccgtcgtt atccttcagc agacgaatgg caaacaggct
     4561 gctgttgttc atcataaact tcgcgccgct gcggtgcacc ttgcgcaggg tgtaaatcag
     4621 tttaatgatc gcatcagcgg tcacacctga agcagcacct gaagcgatgt gctgaagttt
     4681 gccaaacgca cgggtcttgt catcttcatc ggtggactca taagccagaa aaccttttgg
     4741 ttttttgctg ccgtcgccac tggtaaaggc aatttcttcc tgttcggcaa attccagtgc
     4801 cagctcactg ttgatccagt cttccacatt gaaaaaagca tcatcgagca ttttctgggt
     4861 ggcctgcggg ttgccgtaga tttcccccat aaagggttca atcagcccca attttgaggt
     4921 ggcggtttcc ggacgtgcat ccgtttcccc cacccatccg gacgttgtgc cgcccagatt
     4981 caccagtttt ttataatccg agccaccgag ggtgatcacg gtggcttcct ggcgcatcac
     5041 cacctcatct ttcagcaacg tcagaatggt gcggtccagt tcttccggaa tggcataacc
     5101 accatcttca tcattgccca cctgcatcgc cttacgctca agctcacgca ggccgtcttc
     5161 acgtcccttg cgcataaatc cgataaacgc ttctttgtgt tcaccggcaa ctttattttg
     5221 cgtgccgcct gccggacgct tgacttcagc cagttcagct tcaagatcgc ttttcagatt
     5281 ttccagttca gccagttttc cgttgagggt ttccacttca ccagcaagtt ttcccttttc
     5341 ctgctcgatc gcgtcgatac gtttgtcgtt tttttcctta aaatcgtcaa atttcgcctg
     5401 cagctcctgc gcgacctgct ccacatcttt aatatcagcc attattttct cctggttaaa
     5461 atttaagatt tttcagtgca ttcagtgcgg catccacatc ctcagcatca cgcagggata
     5521 aagcgccata tcccccggcc atgaatgctt tggcctgggt tcgcgaaagt ccaacatcgc
     5581 gcaggacccg ctcaataatt ttctgatcag ggatctcccc acgcgccagc gcatttttca
     5641 catcgctgat gcatgcctca tcattggaag ggaacgtcac cagactgacc tcccacaggt
     5701 cgatctcttt cagcaggaat accccttttt cccggtcgta ctcccagtct ttcaggatgt
     5761 agccaataga aaggccggtt aaagaaccgg ccttcatatg ggcatgtgca cgttttgcca
     5821 ggggatcatc atcaacgagt aatcgccccc tgacataaag cccgacatca tcttctttca
     5881 tttcggtgta cacaccgatg ggctcatcca tgcggtgctg ccagagcaac gcaggcaacg
     5941 cctttttttc gctccatttc tggagtgttg tggtaaaggc tccggggacc accacatcat
     6001 cgtggctgtc cttaacacca aaaacagaac cgtaaccttc aaattccccg gaatcactga
     6061 cggatttcag gttcagcggt atatcaagac gctgttttgt ctgcatctcc actctccttt
     6121 ttcttaccgt tatcatcgcc agcagaggga ctggtggtca tgttcatcgg tgtcagatac
     6181 acatcaccgc ccggtcgggg attcatatct tccagatcac ggcagtcatt aggggaataa
     6241 atcccccagt tgatccccgt ggcatacgct tcaaagcggg atttcatgtc gccacgcaac
     6301 aatgctccgg cattaaattt ggcgtaaaac ttcccctgtt tgctctccct gaccagccct
     6361 gtattgatcc gctgctcaat acgggtcaga tacggcacaa gggaatagtt aatgaaacca
     6421 agccccagct cttcaatatt gttgaaggtg gctcgatcgg tgttctgcac catatgcagc
     6481 ggcacacgga acagacgaca gatttcttcc agctgaaact tgcgagtttc caggaactgg
     6541 ctgtcctcgg cgttcagcgc catcgacttc cagtccagcc ccatttccag aatcatcggg
     6601 cggtgagcat tgccaagccc cgtgtgacgc tcctcaaaat ctttcttcag acgctcgtaa
     6661 gcatccggcg tgagcttttg ttccgtacgc aacacaccgg atgtcaccgc accattacca
     6721 aacaacctgg caccgtgctc ctcggttgcc gctgccagtg aaatggcctc acgcgcatac
     6781 gcaatgggat tcagccccac cagtccgtcc agcgtcagag tgcgcacatg ccagatttca
     6841 tcctgggtca gcacatccac ggaaccatca ggaaacgtca cctgataaac cggctgccac
     6901 tggctgttca gcttcggttc cacacagccc ggatctatcg gaagaagctc caccacctcc
     6961 cccaatgcct ttaccttgta ggcgtaaaaa ttcccccgca aacacaggca gacaatgacc
     7021 agctcccaga attcctgcgg cgtcatgtag ccattgggtt ttgccgaaat cagcttatgc
     7081 agtcgttcat ccaccgcccg tgttttaagg gtgccgctga ttttgtagag actgcagggc
     7141 agcataccaa cagactcagc cagcaccctg acgcaggaat agaccgccgt cagccgcatg
     7201 gcccgctggc tgctgatccg ctttccggta taggtgtcgt atgacagccc gatagcctcc
     7261 gccagctctg ctggcgtggt caccggtgcg tcactttttc gttgaaataa ccccgaaaag
     7321 aacactattt acctccgccg acagactgcc gtgtacggtc gagatatcgt gcaaccagcc
     7381 acgaccagaa caggcacagc gccccggcaa caacaaaacc cgccggggga taaatcagcc
     7441 aggcgccata cgccagcaaa agcacaccca gcacgcccac cagaggcgtg agaattatca
     7501 gaaacataat gccctcggtt aaagcgagcg gataccaaca ctaatcaggt gttcagacag
     7561 atccggctcc ggttcaccgc cattgaccag catccggctc attgctgtaa acatcgcaac
     7621 aggaccgtcg attttggctt ccggcgtgga tttgttcggg aagatattgt cgtttttgtc
     7681 cggttttacc gtaacgttag acatcatcca gttcatgacc gggtgattgc tgtggtgaaa
     7741 acgcccggca tagaccagtg attccgtttc cttcatggcc tctgacagat tgcggaccgt
     7801 ctgcggaact tccaccagcg gtatcccttc ttcagccaga gccagactga actgcattgc
     7861 actccacggg tcaaatccca gttccctgag gttttcacca gcaatccatt ccagtaagtc
     7921 actttttatc tgagcatgat cgataacatc accatccgtc aggatgagct tatccatctc
     7981 cgcccacttc cggtaaagtt ctgcctgctg ccgcgagcac cgctccagcc gtccttccgg
     8041 gagccagaat ttaaaatcag catgaacatg tccgttatcg gttcgccaga gttttgccgc
     8101 cgcacagata tcaatcttat gagcaaggtc aacgcctacc cacataggat acgttttcag
     8161 ctcatgtcgt ggagcaatgt attcgcactt ctcccactta atcatgtcca tccaggcaga
     8221 ctcggcagtg acccacacat tcatgtgttt cgtgagaata tttcaagttt tttaaaatca
     8281 cctgtatatc aattgtttaa catgaaaaaa cagtctttat aaatcatcaa gcaatacaca
     8341 gcgcaataca cgtatctgta ttgttttaat gctgatacac accgtttttt gatatagtga
     8401 ttcgacctac catcgcgaag cattttttat ttcttgtgtg taacaatctg acgaggtgag
     8461 cactcaaaga gataagaggc agtagaaatt tcgatccccc ccctgaatgc aa
//
LOCUS       DAZFFG010000165         8361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFFG010000165 DAZFFG010000000
VERSION     DAZFFG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.166"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            69..1403
                     /gene="adeQ"
                     /locus_tag="ACHJ7J_004492"
                     /gene_synonym="yicO"
     CDS             69..1403
                     /gene="adeQ"
                     /locus_tag="ACHJ7J_004492"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO5002623.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1456..1908
                     /gene="yicN"
                     /locus_tag="ACHJ7J_004493"
     CDS             1456..1908
                     /gene="yicN"
                     /locus_tag="ACHJ7J_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO5002624.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2024..2347)
                     /locus_tag="ACHJ7J_004494"
     CDS             complement(2024..2347)
                     /locus_tag="ACHJ7J_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5002625.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LDVLL"
     gene            complement(2331..2684)
                     /locus_tag="ACHJ7J_004495"
     CDS             complement(2331..2684)
                     /locus_tag="ACHJ7J_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5002626.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2918..4102
                     /gene="nepI"
                     /locus_tag="ACHJ7J_004496"
     CDS             2918..4102
                     /gene="nepI"
                     /locus_tag="ACHJ7J_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO5002627.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            4304..>8361
                     /gene="air"
                     /locus_tag="ACHJ7J_004497"
     CDS             4304..>8361
                     /gene="air"
                     /locus_tag="ACHJ7J_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HFO5002628.1"
                     /translation="MAGKAHGNGERRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINHAHSDTATSLILPNVKTIPYTLGALETPPTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEVAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDDRTQTNHGIGWRYFTPSWMSGVNMFIDHDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKPTLSLADSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQVKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGVIKVTVTL
                     KDSYENLVGGQRDAINLAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SDWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKVNQSSDSKT
                     VNFVADVSTAQIADLVVIKDGSVADGSTANTLRVRVTDAFGNALDGQTVSVLAGNSAT
                     VAPTVITEPDGTVEISVTSQTAGVSAVTASINNSSLSQSVMFVADIRTAQIADLEVIK
                     DGSVADGSTANTLRVRVTDAFGNTLAGQTVSVLADNNATVAPTVITEPDGTVEISVTS
                     QTAGTSTVTASINSSSQSRNVTFIADVRTAKIA"
BASE COUNT         1956 a         1991 c         2273 g         2141 t
ORIGIN      
        1 tcactgaacc gttgttgtca tttgttttac aaaagcaagg gattaaatct cctccaatgg
       61 acaaaaaaat gaataatgac aataccgatt acgtgagtaa tgaatcaggg acgctttcgc
      121 gattatttaa actacctcag catgggacca ccgtccgcac agaattgatt gcggggatga
      181 ccactttttt aaccatggtg tacatcgttt ttgtgaaccc gcaaatcctc ggcgcggcac
      241 aaatggaccc gaaagtggtg tttgttacca cctgtttgat tgccggtatc ggcagtattg
      301 cgatggggat atttgctaac ttacccgtgg cgctggctcc ggcaatgggg ctgaacgcct
      361 tctttgcctt cgtggtcgtg ggggctatgg ggatctcatg gcagaccggg atgggcgcga
      421 tattctgggg tgcaattgga ctatttttgc tcacgctgtt tcgtatccgg tactggatga
      481 tctccaacat tccattaagt ttacgtattg gtatcaccag tggaattgga ttatttatcg
      541 ccttaatggg attaaaaaat actggcgtta ttgtcgccaa taaagacacc ctggtgatga
      601 ttggcgattt aagttctcac ggtgtgttgt taggtatttt agggtttttt attataaccg
      661 tgttgtcatc acgtcatttt catgccgcgg tgctggtttc tattgtggtg acgtcttgct
      721 gtggattatt tttcggtgat gttcatttta gcggcgtcta ttccattccg cctgatatta
      781 gcggcgtcat tggtgaagta gatttgagcg gcgcgttaac acttgaactc gccggtatca
      841 ttttctcctt tatgctgatc aacctatttg attcatcagg aacattaatt ggtgtaactg
      901 ataaagctgg cttaatagat agtaacggta aattccccaa tatgaataag gcgctgtatg
      961 ttgatagcgt cagttcggtg gcgggcgcgt ttatcggtac ctcgtctgtt actgcctata
     1021 ttgaaagtac ttctggtgtg gcagtcggtg gtcgcacggg gctaacggct gtcgtggtcg
     1081 gcgttatgtt cctgttagtt atgttcttct caccgctggt ggcgatggtt cctccttacg
     1141 caaccgccgg agcgttaatc tttgttggcg tgctgatgac ttcgagcctg gcgcgcgtta
     1201 actgggatga ttttaccgaa tcagtgcccg catttattac cacggtgatg atgcccttta
     1261 ctttctcgat caccgaaggg attgcactcg gctttatgtc gtactgcatc atgaaagtat
     1321 gcaccgggcg ctggcgcgat ctgaacctgt gtgtggtggt ggtcgcagct ctgtttgcac
     1381 tgaagattat tctggtggat tagtaactgg cgagggtttc cagatatcat gagttctgat
     1441 tacgcaggag aactcatgat ctggataatg ctcgccacgc tggcggtagt gtttgtggtt
     1501 ggttttcggg tgctgacatc cggggccaga aaagcgattc gccgtctcag cgatcggctg
     1561 aatatcgatg tcgtacccgt ggagtcgatg gtcgatcaaa tgggaaagtc agctggtgac
     1621 gaatttttac gttatttgca tcgtccggat gagtcgcacc tgcaaaacgc cgcgcaggtg
     1681 ttgctcatct ggcaaattgt cattgtcgat ggtagcgaac agaacctgct gcaatggcat
     1741 cggattttac aaaaagcccg ccttgccgcg ccgattaccg atgctcaggt caggctggcg
     1801 ctgggttttc tgcgcgaaac tgaacctgaa atgcaggata ttaatgcttt tcagatgcgc
     1861 tataacgcat tctttcagcc tgccgagggc gttcactggc tgcattaaaa ccgcttcgtc
     1921 ttatcagact acagaccgtg cctgacctgt cgagtggata aggcgttcac gccgcgtccg
     1981 gtatcggaac tgacagtagg tgaacgcatt atctacgttc tatttacagc agcacatcca
     2041 gcccgtgttt ttgcacaatg ctgagcagtt ttaatgaaat gccattgggg cgtttagcgc
     2101 cactttccca tttttgtaca gttgaaacgc tggtattgag atagtgagcg aagacgggct
     2161 gactcacttt taatttttcc cgcagtgctt ttatctcaac aggttgtaag tcttgcacgc
     2221 tggtcaggca ggctttgtca aaagtacgca ttgtttcggc agaaatagca tcgacgctgg
     2281 acaatccagc cgctgcgctg tgaatagcct caaatgcagg acttttaaat ttagtttttg
     2341 cagtcatagc agatctctgt cagttcttta gtgttaatca ttgctgttag ctgggctttc
     2401 gtcaggaagg cataatgctt tgcaagttca cgaaacccag ccagttcctg actattgatg
     2461 ttcgacatat cctgttttgc atataaaaac gtgtaaaacc aattgcttcc cccttttgcc
     2521 agaataattg cacgctcacg attttggtga aggcgctttt taaaaacgcc accaccaagt
     2581 gcatcagctt taccttgctc cgtttctaaa atggcccggc acaactcttc atctgttatt
     2641 gcatgagacc tggcttccct tgcaaaccat ttggttttaa acacgcgcat atcgttgagg
     2701 tcctgttatt aaagtatatc accaagtgca atactttatc ccttattccc aacccaatcg
     2761 cgaaaatgca tatcagcttg ccctatgatt tttatttctt cgagcagcct cgaacttttc
     2821 ccacaacacc gtaaaagttg caaaaacgtt aaatgcgtca cacatttcaa tgttaaagtg
     2881 gtcggctttt cccctgaaac atgccacggg taacaccatg agtgaattta ttgccgaaaa
     2941 ccgcggcgcg gatgccatca cccgaccgaa ctggtcagcc gttttctcgg tggcgttttg
     3001 tgtcgcctgt ctgattatcg ttgagttttt gcccgtcagt ttgttgacgc caatggccca
     3061 ggatttaggc atttcggaag gggttgccgg gcaatcggtg accgtgaccg cctttgtggc
     3121 aatgtttgcc agtttgttta ttacccagac aattcaggcc actgaccgcc gctacgttgt
     3181 tattttattt gccgttttgc tgacgctctc ctgtttgctg gtttcctttg ctaactcatt
     3241 cagtttgctt ttaattggtc gtgcctgtct ggggctggcg ctgggcgggt tctgggcgat
     3301 gtcggcgtcg ctgaccatgc gtctggtacc gccgcgtacg gtgccgaagg cgctgtcggt
     3361 gatcttcggc gcggtttcta ttgcgctggt gattgccgca ccgttgggca gttttttagg
     3421 cgagcttatc ggttggcgca atgtctttaa tgcggcggcg gtgatgggcg tgctgtgtat
     3481 tttctggatt atcaaatcat tgccttcgct gccaggcgaa ccctcgcatc agaaacaaaa
     3541 tactttccgc ttattacaac gtccgggtgt gatggcaggg atgatcgcca tcttcatgtc
     3601 tttcgccggg cagtttgctt tcttcacgta tattcgcccg gtgtatatga acctggcggg
     3661 attcggcgtg gatggcttaa ctctggtgct gttgagtttt ggtatcgcca gctttattgg
     3721 tacgtcgctt tcgtcgttca ttcttaaacg ttcggtaaaa ctggccttag caggcgcgcc
     3781 gttaatactg gctgtgagtg cgttggtgct gacgttgtgg ggaagcgata aaatcgttgc
     3841 taccggcgtg gcgattatct gggggctaac ttttgcattg gttcctgtcg gctggtcaac
     3901 gtggatcacc cgctcactgg ccgatcaggc agaaaaagcc gggtctattc aggtggcggt
     3961 tattcagctt gctaatacct gtggcgcggc aatcggcggt tatgcgctgg ataatattgg
     4021 tctgacttcg ccgttgatgt tgtccggcac attgatgttg ctgacggcgt tattggtcac
     4081 cgcaaaggtg aaaatgaagt aacgtattta caggagggtt atttctctgg cgggaaagct
     4141 ctcctgttct gccccctgat aacgcttgat caggtattgt tcttatcttg ttgttatttc
     4201 cattattccg cctgtattca tcatttgcta atcatgaatc atcattttcc ctcatgttaa
     4261 agaaaggtca tttaatctga acgcatagcg acagggatga caaatggcag ggaaagcgca
     4321 tgggaatggc gagcgtcggg gcgataacac catatgtgga ttgggggatc gtttacgtcg
     4381 tctgaccgcc ggtatttgcc tgattacaca aactatattc cctgttatgg ccgctgcccc
     4441 tacgcatatt aatcatgcgc attcggatac tgccacgtcc ctgatcctgc cgaacgttaa
     4501 aactattcca tacaccctgg gggcattgga aacgccacca acggtcgcgg cacgctttgg
     4561 tatcactgtt gatgaattac gtcgcctgaa tcagtttcgt acctttgcgc ggggctttga
     4621 caatgtgcgt cagggcgatg aaatagatgt gcccctcatt aacagtaata gccctgaagc
     4681 gcgcaacctg aaagcaatgc agatggagcg cgacggcaaa gatccgcaaa tgcaggtcgc
     4741 ggaagtggcg cagcaaagcg gaacactttt agctcgcgat atggacagcg aacaggcggc
     4801 gagtatggcg cgtggctggg tggcttcttc tgcatctgcg caggcaacgg actggctcag
     4861 tcgttgggga actgcccggg tatctcttgg tgtggatgaa gatttcagcc ttaaaagctc
     4921 ttcatttgaa tttctccatc cctggtacga aacgccggat aacctggtct tcagccagca
     4981 cacacttcac cgtacggatg atcgcactca aaccaaccac ggtatcggct ggcgttattt
     5041 caccccgagc tggatgtctg gcgtcaatat gtttatcgat catgacctga cccgctatca
     5101 cacccgaacc gggatgggcg ttgaatactg gcgcgattac ctgaaactga gcggcaacgg
     5161 ttatctgcgc ctgagtaact ggcgtagtgc accggaactc gacaatgact atgaagcacg
     5221 tccggcgaac ggttgggatc ttcgcgccga aggctggtta ccggcctggc cgcagctggg
     5281 cggcaagctg gtctatgaac aatattatgg cgatgaagtg gcgctgtttg gtaaagacga
     5341 gcgacaaaat gatcctcacg cgattaccgc aggtttgagt tacacccccg ttcccttaat
     5401 ctcctttagt gccgagcaac ggcagggcaa acagggagaa aacgacaccc gaattggtat
     5461 ggagttgacg ttgcaacctg gtcattcact gcaaaaacag ctcgatccag ctgaagttgc
     5521 agctcggcgc agccttgtgg gtagtcgcta tgacctggtc gatcgtaata acaatatcgt
     5581 cctcgaatat cgcaagaaag agctggtacg gctgacgctg accgatccgc tgaaaggcaa
     5641 gccgggtgag gttaaatcgc tggtttcttc cttacagacc aaatatgcgc tgaaaggtta
     5701 tgacatcgaa gccgcctccc tgcaatcagc gggcggaaaa gtagcagttt ccggcaagga
     5761 tattcaggtc actattcctc cctaccgatt cacggcaatg cctgaaaccg acaacactta
     5821 cccgatagcg gttacagcgg aagattcgaa aggaaatttc tcccggcgtg aagaaagtat
     5881 ggtggtcgtg gaaaaaccca cactaagcct cgccgattca acgctttctg tcgatctgca
     5941 aatcctgctt gctgatggaa aatcaacctc aacgctgacc tataccgcgc gagattccag
     6001 cggcaagcca atcccaggta tgacgttaaa aacacaggtg aaaggtttgc aggatttcgc
     6061 cttaagcgaa tggaaagaca acggtaacgg aacctatacc caaatagtta ccgccgggaa
     6121 gacgtctggc gcgttatcgc tgatgccgca atttaacggc gacgatatcg ctaagacgcc
     6181 agcgctgatt gctatcgtgg cgaatacggc atcccgcgct gactccacga ttgagacaga
     6241 ccaggataat tacgtcgcgg gtaagccgat agtcgttaaa gtgaccctga gagatgataa
     6301 cggcaacggg gttaccggac gaaaagaact tctcaaacaa accgttaaag tagacaacac
     6361 gaaggctgat gccgttagtg cctggacgga agaaagtgag ggaatttaca aagcaagcta
     6421 tacggcccat ctcattggcg acaaattaac ggcccagctg acgatgcccg gctggcaaac
     6481 caaacattcg gatgcgttca gtattgcggg tgataaagat accgccaaaa tcgctgcgat
     6541 gcagatcacc gcgaataacg cagttgccag acgcgatcac aataccgtcg ctgtgacggt
     6601 cagggatgtg catcaaaatt tactgcaggg acaaaatgtt accttcacgg tcgtgaatgg
     6661 tgcggcggtt tttgccgatc cgaatggcgg tattgtgacg acagataaag acgggatagc
     6721 cagcgttaac cttgccagtg accaggcggt aaacagtctt atcaaggccg agatcaatgg
     6781 cagtagtcag tctgtggaag tgagctttat cactggcgat atctcgcagc tgacctccac
     6841 catcaagacg gacgatgtgt catacactgc gggtggcaag ataaaagtgt ccgtcaccct
     6901 tatggatgag cagaaaaacc tggtgaaagg gatggcttcg ctgctggcgg gtagcggtgt
     6961 ggtcgaggtg agcggtacag ataaaaatga aacgggtaac tggagcgaag agtctgacgg
     7021 cgtttacacc accacccgaa ccgcgaagat tgctggcgat cgccattacg ccacgctgaa
     7081 actatcgaca tggagtagcg cgcagcaatc cgatgcgtat gccatccgcg aaagcggagc
     7141 tgtgctggct tattcctcca tcgttaccga caaaacgacc tacaccgcgg gcggcgtgat
     7201 aaaagttacg gtaacactca aggatagcta tgaaaacctg gtcggggggc aacgagacgc
     7261 tatcaacctg gctatccagc tgccaaatac aaaagcggaa agcatcgcct ggaatgaaga
     7321 tcaaaaagga atttataccg caacgtatac cgccttactc cctggcaccg gtttaaaagc
     7381 acagctacag atgtccgact gggcgagcgc tctgacttca aatgattaca gtattagtgg
     7441 cgatgccgcc agcgcacaaa ttgtcgcgat gcaagtgaca acgggtaacc cagatgtgct
     7501 ggcgaatggc agcgatcgac atacggtgaa tgtccgggtg gaagatcagt ttggcaatgt
     7561 gctgccggag cagaccgtca cctttacggt gacaaagggg gcggctgtat ttgccaatgc
     7621 agggcagagc gcagatatca ggacggatgc gcatggtatg gcagaggttg atctgagcag
     7681 taccgtagcg gatgccagca ctgttgaggc taaggtcaat caaagcagcg atagtaaaac
     7741 ggtgaacttt gttgctgatg tcagcacggc gcagatcgct gatctggtgg tcattaagga
     7801 tggctcagtg gcagacggtt cgacggcgaa cacgctgcgg gtgagggtga cggatgcgtt
     7861 cggtaatgcg ctggacgggc agacggtcag cgtattggca ggcaacagcg caacggtcgc
     7921 tccgacggtt atcaccgagc cggacgggac ggtggagatc agtgtcacca gtcagacggc
     7981 gggagtcagt gcggtcaccg ccagcatcaa caacagcagc ctgagccaga gcgtgatgtt
     8041 tgtcgccgat atcaggacgg cgcagatcgc tgatctggag gtcattaagg acggctcagt
     8101 ggcagacggt tcgacggcga acacgctgcg ggtgagggtc acggatgcgt tcggtaatac
     8161 gcttgccgga cagacggtca gcgtgttggc ggacaacaac gcaacggttg ccccgacggt
     8221 gattacagag ccggacggca cggtggagat cagtgtcacc agccagacgg cgggtaccag
     8281 tacagtcacc gccagcatca acagcagcag ccagagccgg aacgtgacgt ttatcgccga
     8341 tgtcaggacg gcgaagatcg c
//
LOCUS       DAZFFG010000166         8325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFFG010000166 DAZFFG010000000
VERSION     DAZFFG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.167"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..697
                     /gene="yneK"
                     /locus_tag="ACHJ7J_004498"
     CDS             <1..697
                     /gene="yneK"
                     /locus_tag="ACHJ7J_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO5002629.1"
                     /translation="FQTGVERVLFLFLNDFIEQFPMINPGVPIKRAHTPHIEPLPPDH
                     HTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITAILDITDSEL
                     KEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDLFLFFANMLLHYHQFMNP
                     NESDLNNVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNRPRSPVRSPI
                     PATSKTPDLPERH"
     gene            847..2037
                     /gene="ydeA"
                     /locus_tag="ACHJ7J_004499"
     CDS             847..2037
                     /gene="ydeA"
                     /locus_tag="ACHJ7J_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO5002630.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2062..2727)
                     /gene="marC"
                     /locus_tag="ACHJ7J_004500"
     CDS             complement(2062..2727)
                     /gene="marC"
                     /locus_tag="ACHJ7J_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO5002631.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2939..3373
                     /gene="marR"
                     /locus_tag="ACHJ7J_004501"
     CDS             2939..3373
                     /gene="marR"
                     /locus_tag="ACHJ7J_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO5002632.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3394..3777
                     /gene="marA"
                     /locus_tag="ACHJ7J_004502"
     CDS             3394..3777
                     /gene="marA"
                     /locus_tag="ACHJ7J_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO5002633.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3809..4027
                     /gene="marB"
                     /locus_tag="ACHJ7J_004503"
     CDS             3809..4027
                     /gene="marB"
                     /locus_tag="ACHJ7J_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO5002634.1"
                     /translation="MKPLLSAIAAALTFFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4058..4957)
                     /gene="eamA"
                     /locus_tag="ACHJ7J_004504"
     CDS             complement(4058..4957)
                     /gene="eamA"
                     /locus_tag="ACHJ7J_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO5002635.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVVVLIEDSFNGQHVAMLGFILTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSATMIHSLVTIDLTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5152..6339
                     /gene="ydeE"
                     /locus_tag="ACHJ7J_004505"
     CDS             5152..6339
                     /gene="ydeE"
                     /locus_tag="ACHJ7J_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO5002636.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKKKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            6466..6561
                     /gene="mgtS"
                     /locus_tag="ACHJ7J_004506"
     CDS             6466..6561
                     /gene="mgtS"
                     /locus_tag="ACHJ7J_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO5002637.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            6561..6665
                     /gene="mgtT"
                     /locus_tag="ACHJ7J_004507"
     CDS             6561..6665
                     /gene="mgtT"
                     /locus_tag="ACHJ7J_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO5002638.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(6780..7670)
                     /gene="dgcZ"
                     /locus_tag="ACHJ7J_004508"
     CDS             complement(6780..7670)
                     /gene="dgcZ"
                     /locus_tag="ACHJ7J_004508"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO5002639.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FAAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDIVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(7925..8317)
                     /gene="ydeI"
                     /locus_tag="ACHJ7J_004509"
     CDS             complement(7925..8317)
                     /gene="ydeI"
                     /locus_tag="ACHJ7J_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO5002640.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHQGEDRYVFRDKSGEVNVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         2200 a         2045 c         1851 g         2229 t
ORIGIN      
        1 atttcagacc ggcgtcgagc gtgtgctctt tctgtttctg aatgatttta ttgaacaatt
       61 tccaatgata aaccctggcg ttcccatcaa aagagcgcat acaccacata ttgaacccct
      121 gcctccggat caccatactg ctgcagatta cttacgccag tttgatttgc ttgtcctgaa
      181 ttttatctct cgcggtaatt ttgtcatact cccccgatta tggaataact ctgaggttca
      241 cagatggttc gtcaataaag atcctaactt gatcaccgcc attctcgaca taacggacag
      301 cgaattaaaa gaggatttat tgcaaagcct gatggattca ttaggatcta acaaacatgt
      361 actacccgaa gtctgcatct gctttttatc ccttttagca gaacaagaat ctcctcattt
      421 tcaggacttg tttttatttt tcgccaatat gttattgcac tatcaccaat ttatgaatcc
      481 caatgaaagt gatttgaata acgtgttgat gccagcatca ttaagtgatg ataaaattat
      541 caaacatatg gcgcgcagga ccctcaaact ttttgtaaaa aatgaaacgc cgccaaaagt
      601 tactcatgaa gacctggtga aaaacaggcc tcgctcccct gtcagatcac ctatacccgc
      661 aacctccaaa acgccagacc tccctgaacg tcattaaacc gtgatgtgac cgactctctg
      721 acgcgtgaaa gaatcagcgt cagagaaacg gaaaacgcga tccagatcac aaatgcattg
      781 tattcacatc attaaccgtt ttaagatcat ttcatcactt tttcgcaact cacccgataa
      841 tctgttatga caacaaacac tgtttcccgc aaagtggcgt ggctacgggt cgttacgctg
      901 gcagtcgccg ccttcatctt caacaccacc gaatttgtcc cagttggcct gctctctgac
      961 attgcgcaaa gttttcacat gcaaaccgct caggtcggca ttatgttgac catttacgca
     1021 tgggtagtag cgctaatgtc attgcctttt atgttaatga ccagccaggt tgaacggcgc
     1081 aaattactga tctgcctgtt tgtggtgttt attgccagcc acgtactgtc gtttttatcg
     1141 tggagcttta ccgttctggt gatcagtcgc attggtgtgg cttttgcaca tgcgattttc
     1201 tggtcgatta cggcgtccct ggcgatccgt atggctccgg ccgggaagcg agcacaggca
     1261 ttgagtttaa ttgccacagg tacagcactg gcaatggtat taggattacc tctcgggcgc
     1321 attgtggggc agtatttcgg ttggcgaatg accttcttcg cgattggtat cggggcgctt
     1381 gttacccttt tgtgcctgat taagttactt cccttactgc ccagtgagca ttccggttca
     1441 ttgaaaagcc tcccgctatt gttccgccgc ccggcattga tgagcattta tttgttaact
     1501 gtggtggttg tcaccgccca ttacacggca tacagctata ttgagccttt tgtgcaaaac
     1561 attgcgggat tcagcgccaa ctttgccacg gcattactgt tattactcgg tggtgcgggc
     1621 attattggca gcgtgatttt cgggaaactg ggtaatcagt atgcgtctgc gttggtgagt
     1681 acggcgattg cgctgttgct ggtgtgtctg gcactgctgc tacctgcggc gaacagtgaa
     1741 atacacctcg gggtgctgag tattttctgg gggatcgcga tgatgatcat cgggcttggt
     1801 atgcaggtta aagtgctggc gctggcacca gatgccaccg acgtcgcgat ggcgctattc
     1861 tccgggatat ttaatattgg aatcggggcg ggtgcgttgg taggtaatca ggtgagtctg
     1921 cactggtcaa tgtcgatgat tggttatgtg ggcgcggtgc ctgcttttgc cgcgttaata
     1981 tggtcaatca ttatatttcg ccgctggcca gtgacactcg aagaacagac gcaatagttg
     2041 aaaggcccat tcgggccttt tttaatggta cgttttaatg atttccagga tgccgttaat
     2101 aataaactgc acacccatac ataccagcag gaaccccatc aggcgggaga tcgcttcaat
     2161 gccacccttg cccaccagcc gcataattgc gccggagctg cgtaggcttc cccacaaaat
     2221 aaccgccacc aggaaaaaga tcagcggcgg cgcaaccatc agtacccaat cagcgaaggt
     2281 tgaactctga cgcactgtgg acgccgagct aataatcatc gcaatggttc ccggaccggc
     2341 ggtacttggc attgccagtg gtacaaaagc aatattggcg ctgggttcat cttccagctc
     2401 ttccgacttg cttttcgctt ccggtgaatc aatcgctttc tgttgcggaa agagcatccg
     2461 aaaaccgata aacgcgacga ttaagccgcc tgcaattcgc agaccgggaa tcgaaatgcc
     2521 aaatgtatcc atcaccagtt gcccggcgta atacgccacc atcatgatgg caaagacgta
     2581 caccgaggcc atcaacgact gacgattacg ttcggcactg ttcatgttgc ctgccaggcc
     2641 aagaaataac gcgacagttg ttaatgggtt agctaacggc agtaacacca ccagccccag
     2701 gccaattgct ttaaacaaat ctaacattgg tggttgttat cctgtgtatc tgggttatca
     2761 gcgaaaagta taaggggtaa acaaggataa agtgtcactc tttagctaac cttgcatcgc
     2821 attggccaaa acttgaaccg atttagcaaa acgtggcatc ggtcaattca ttcatttgac
     2881 ttatacttgc ctgggcaata ttatcccctg caactaatta cttgccaggg caactaatgt
     2941 gaaaagtacc agcgatctgt tcaatgaaat tattccattg ggtcgcttaa tccatatggt
     3001 taatcagaag aaagatcgtc tgcttaacga gtatctgtct ccgctggata ttactgcggc
     3061 acagtttaag gtgctctgct ctatccgctg cgcggcgtgt attactccgg ttgaactgaa
     3121 aaaagtgttg tcggtcgacc tgggagcact gacccgtatg ctggatcgcc tggtctgtaa
     3181 aggctgggtt gaaaggttgc cgaacccgaa tgataagcgc ggcgtactgg taaaacttac
     3241 caccagcggc gcggcaatat gtgaacaatg ccatcaatta gttggccagg acctgcatca
     3301 agaattaaca aaaaacctga cggcggacga agtggcaaca cttgagcatt tgcttaagaa
     3361 agtcctgccg taaacaaaaa atgaggtatg acgatgtcca gacgcaatac tgacgctatt
     3421 accattcata gcattttgga ctggatcgag gacaacctgg aatcgccact gtcactggag
     3481 aaagtgtcag agcgttcggg ttactccaaa tggcacctgc aacggatgtt taaaaaagaa
     3541 accggtcatt cattaggcca atacatccgc agccgtaaga tgacggaaat cgcgcaaaag
     3601 ctgaaggaaa gtaacgagcc gatactctat ctggcagaac gatatggctt cgagtcgcaa
     3661 caaactctga cccgaacctt caaaaattac tttgatgttc cgccgcataa ataccggatg
     3721 accaatatgc agggcgaatc gcgcttttta catccattaa atcattacaa cagctagttg
     3781 aaaacgtgac aacgtcactg aggcaatcat gaaaccactt ttatccgcaa tagcagctgc
     3841 gcttactttc ttttccgcgc agggcgttgc ggaacaaacc acgcagccag ttgttacttc
     3901 ctgtgccaat gtcgtggttg ttcccccatc gcaggaacaa ccaccgtttg atttaaatca
     3961 catgggtact ggcagtgata agtcggatgc gctcggcgtg ccctattata atcaacacgc
     4021 tatgtagttt gttctggccc cgacatctcg gggcttatta acttcccacc tttaccgctt
     4081 tacgccaccg caagccaaat acattgatat acagcccggt cataatgagc accgcaccga
     4141 aaaattgcag acccgttaag cgttcatcca acaatagtgc cgcacttgcc agtcctacta
     4201 cgggcaccag taacgataac ggtgcaaccc gccaggtttc atagcgtcca agtagtgttc
     4261 cccagatccc ataaccaaca attgtcgcca caaaagccag atacatcaga gacaagatag
     4321 tggtcaaatc gatagtgacc agactgtgaa tcatggttgc ggaaccatcg agaatcagtg
     4381 aggcaacaaa gaagggaata attgggatta atgcgctcca gacgaccaga gacattaccg
     4441 ccggacggga tgaatgagac atgatctttt tgttgaagat attgccactc gcccaactaa
     4501 atgccgctgc cagagtcaaa ataaaaccga gcatcgccac atgctgaccg ttaaagctat
     4561 cttcgattaa caccactacg ccaaaaatcg ctaaggcgat ccccgccaat tgtttgccat
     4621 gcagtcgctc accgaaagta aacgcgccaa gcacgatagt aaaaaacgcc tgtgcctgta
     4681 acaccagcga agccagtcca gcaggcatac caaagttaat ggcgcagaag agaaaggcaa
     4741 actgtgcaaa actgatggtt aatccatacc ccagcagcaa attcagtggt actttcggtc
     4801 gtgcgacaaa aaagatggcc ggaaaagcga ccagcataaa gcgcaaaccg gccagcatca
     4861 gcggtggcat gttatgaagc cccactttga tgaccacaaa atttagcccc catacgacca
     4921 ctaccagtag cgccaacacc ccatcttttc gcgacattct accgcctcta aatttcatct
     4981 tttgtaagca atcaacttag ctgaatttac ttttctttaa cagttgattc gttagtcgcc
     5041 ggttacgacg gcattaatgc gcaaataagt cgctatactt cggatttttg ccatgctatt
     5101 tctttacatc tctaaaacaa aacataacga aacgcactgc cggacagaca aatgaactta
     5161 tccctacgac gctctaccag cgcccttctt gcctcgtcgt tgttattaac catcggacgc
     5221 ggcgctacgc tgccatttat gaccatttac ttgagtcgcc agtacagcct gagtgtcgat
     5281 ctaatcggtt atgcgatgac aattgcgctc actattggcg tcgtttttag cctcggtttt
     5341 ggtatcctgg cggataagtt cgacaagaaa cgctatatgt tactggcaat taccgccttc
     5401 gccagcggtt ttattgccat tcctttagtg aataacgtga cgctggtggt gctctttttt
     5461 gccctcatta actgcgccta ttccgttttt gctaccgtgc tgaaagcctg gtttgccgac
     5521 aatctttcgt ccaccagcaa aaagaagatc ttctcaatca actacaccat gctcaacatt
     5581 ggctggacca tcggtccgcc gctcggcacg ttgttagtga tgcagagcat caatctgccc
     5641 ttctggctgg ccgctatctg ttccgcgttt cccatgcttt tcattcaaat ttgggtaaag
     5701 cgcagcgaga aaatcatcgc cacggaaaca ggcagtgtct ggtcgccgaa agttttatta
     5761 caagataaag cactgttgtg gtttacctgc tctggctttc tggcttcttt tgttagcggc
     5821 gcatttgctt catgcatttc acaatatgtg atggtgattg ctgatgggga ttttgccgaa
     5881 aaggtggtcg cggttgttct tccggtgaat gctgccatgg tggttacgtt gcaatattcc
     5941 gtgggccgcc gacttaaccc ggctaacatc cgcgcgctga tgacagcagg caccctctgt
     6001 ttcgtcatcg gtctggtcgg ttttattttt tccggcaaca gcctgctatt gtggggtatg
     6061 tcagctgcgg tatttactgt cggtgaaatc atttatgcgc cgggcgagta tatgttgatt
     6121 gaccatattg cgccgccagg aatgaaagcc agctattttt ctgcccagtc tttaggctgg
     6181 cttggtgccg cgattaaccc attagtgagc ggcgtagtgc taaccagcct gccgccttcc
     6241 tcgctgttta tcatcttagc gttggtgatc attgctgcgt gggtgctgat gttaaaaggg
     6301 attcgtgcaa aaccgtgggg gcagcccgcg ctttgttgat ttaagtcgaa cacaataaaa
     6361 atttaattca gccttcgttt aggttacctc tgctaatatc tttctcattg agatgaaaat
     6421 taaggtaagc gaggaaacac accacaccat aaacggaggc aaataatgct gggtaatatg
     6481 aatgttttta tggcagtact gggaataatt ttattttctg gttttctggc cgcgtatttc
     6541 agccacaaat gggatgacta atgaacggag ataatccctc acctaaccgg ccccttgtta
     6601 cagttgtgta caaggggcct gatttttatg acggcgaaaa aaaaccgcca gtaaaccggc
     6661 ggtgaatgct tgcatggata gatttgtgtt ttgcttttac gctaacaggc attttcctgc
     6721 actgataacg gatcgttgac acagtagcat cagttttctc aatgaatgtt aaacggagct
     6781 taaactcggt taatcacatt ttgttcgtca ataaacatgc agcgatttct tccggtttgc
     6841 ttaccctcat acattgcccg gtctgctctt ccaatgacca catccagagg ctcttcagga
     6901 aatgcgcgac tgacacctgc tgtcacggta atgttgatat gcccttcaga atgtgtgatg
     6961 gcatggttat cgactaactg gcaaattctg acacctgcac gacatgcttc ttcatcatta
     7021 gccgctttga caataatgat aaattcttcg cccccgtaac gataaaccgt ttcgtaatca
     7081 cgcgtccaac tggctaagta agttgccagg gtgcgtaata ctatatcgcc gattaaatgc
     7141 ccgtaggtat cattaaccaa tttaaatcgg tcaatatcca acaacattaa ataaagattc
     7201 agaggctcag tgttgcgtaa ctgatgatca aaggattcat caagaacccg acgccccggc
     7261 aatcccgtca aaacatccat attgctacgg atcgtcagca aataaatttt gtaatcggtt
     7321 aatgccgcag taaaagaaag taacccttcc tgaaaagcgg cgaaatgcgc gtcctgccag
     7381 tgattttcaa caatagccag cattaattcc cgaccacagt tatgcatatg ttgatgggca
     7441 gaatccatta gccgaacgta aggtaattca tcgttatcga gtggtcccag atgatcaatc
     7501 caccgaccaa actggcacag tccataagaa tggttatccg ttatttctgg cttactggca
     7561 tctctcgcga ctacgctgtg aaacatactt accagccact ggtagtgggc atcgatagcc
     7621 ttattgagat ttaacaagat ggcatcaatt tccgttgtct tcttgatcat tgccactcct
     7681 ttttcacagt tccttgtgcg cgctattcta acgagagaaa agcaaaatta cgtcaatatt
     7741 ttcatagaaa tccgaagtta tgagtcatct ctgagataac attgtgattt aaaacaaaat
     7801 cagcggataa aaaagtgttt aattctgtaa attaccactg cattatcaca aataaaagga
     7861 tgacaaatag cataacccaa tacccaaatg gcccagtagt tcaggccatc aggctaattt
     7921 atttttattt ctgcaaatga gtgacccgaa cgacggccgg cgcgcttttc ttatccagac
     7981 tcccgctaat gttgatcatc tggtcaggct gaacttctcg cccatcaaag acagcggcag
     8041 gaataacaac attaacttca ccgctcttat cgcggaaaac gtaacggtct tctccctgat
     8101 gagaaattaa attcccccgc aatgaaacag aagcaccatc atgcatcgtt ttcgcgaaat
     8161 caacggtcat tttttttgca tcatcggttc cgcgatagcc atcttctatt gcatgaggcg
     8221 gcggtggcgc tgcatcctgt tttaaaccgc cctggtcatc tgccaacgca taaggcatga
     8281 caagaaaact tgctaataca atggcctgaa atttcatact gactc
//
LOCUS       DAZFFG010000167         8325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFFG010000167 DAZFFG010000000
VERSION     DAZFFG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.168"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(104..180)
                     /locus_tag="ACHJ7J_004510"
     tRNA            complement(104..180)
                     /locus_tag="ACHJ7J_004510"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Asp,seq:gtc)
     gene            complement(313..1044)
                     /gene="dnaQ"
                     /locus_tag="ACHJ7J_004511"
     CDS             complement(313..1044)
                     /gene="dnaQ"
                     /locus_tag="ACHJ7J_004511"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO5002641.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1109..1576
                     /gene="rnhA"
                     /locus_tag="ACHJ7J_004512"
     CDS             1109..1576
                     /gene="rnhA"
                     /locus_tag="ACHJ7J_004512"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO5002642.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1573..2295)
                     /locus_tag="ACHJ7J_004513"
     CDS             complement(1573..2295)
                     /locus_tag="ACHJ7J_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5002643.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2329..3084
                     /gene="gloB"
                     /locus_tag="ACHJ7J_004514"
     CDS             2329..3084
                     /gene="gloB"
                     /locus_tag="ACHJ7J_004514"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO5002644.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3156..4514
                     /gene="mltD"
                     /locus_tag="ACHJ7J_004515"
     CDS             3156..4514
                     /gene="mltD"
                     /locus_tag="ACHJ7J_004515"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO5002645.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTLLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4562..5332)
                     /locus_tag="ACHJ7J_004516"
     CDS             complement(4562..5332)
                     /locus_tag="ACHJ7J_004516"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5002646.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5410..6210)
                     /locus_tag="ACHJ7J_004517"
     CDS             complement(5410..6210)
                     /locus_tag="ACHJ7J_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5002647.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6451..7365
                     /gene="yafC"
                     /locus_tag="ACHJ7J_004518"
     CDS             6451..7365
                     /gene="yafC"
                     /locus_tag="ACHJ7J_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO5002648.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7362..8165)
                     /gene="dkgB"
                     /locus_tag="ACHJ7J_004519"
     CDS             complement(7362..8165)
                     /gene="dkgB"
                     /locus_tag="ACHJ7J_004519"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO5002649.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLGKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2217 a         1993 c         2029 g         2086 t
ORIGIN      
        1 cataacaaaa tcatagaccg aagaaatagc gggatttaaa tgaaaaaaac cgcaaaatta
       61 aaaatcttgc ggttctctga acctcatttt catgaggtga atttggcgga acggacggga
      121 ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc
      181 gcgttgtgtt ccgttgggaa cgaggcgaat attacggatt gcctcacccg tcgtcaatgg
      241 tttttctcat cttttgaatc gtttgctgca aaaatcgccc aagtcgctat ttttagcgcc
      301 tttcacaggt atttatgctc gccagaggca gcttccgcct ttcttctgca ccagatcgag
      361 acgggcttca tgtgctgcaa gctcttcatc tgtcgcaaaa acaacgcgta acttacttgc
      421 ctgacgtaca atgcgctgaa ttgttgcttc accttgttgc tgttgtgtct ctccttccat
      481 cgcaaaagcc atcgacgttt gaccaccggt catcgccaga taaacttccg caaggatctg
      541 ggcatcgagt aatgccccgt gtagcgttcg tttactgtta tctatttcgt agcgggcaca
      601 taacgcatcg aggctgttgc gcttaccagg aaacattttc ctcgccaccg caaggctatc
      661 ggtgacctta cagaaggtat tggtcttcgg aatatcgcgc ttaagcaacg agaactcgta
      721 gtccataaag ccgatatcga acgctgcgtt atggatcacc aactccgcgc cgcggatata
      781 gtccatgaac tcatcggcta cttcggcaaa cgtgggctta tcgagcaaaa attcatcggc
      841 aataccatgt acgccaaagg cttccggatc caccagccga tcgggtttga gataaacatg
      901 gaagttattg cccgtcaggc gacggttcac cacttcaacg gcaccaatct caatgatctt
      961 gtggccttca tagtgcgcac caatctggtt cataccggtg gtttcggtat cgagaacgat
     1021 ctggcgtgta attgcagtgc tcatagcggt catttatgtc agacttgtcg ttttacagtt
     1081 cgattcaatt acaggaagtc taccagagat gcttaaacag gtagaaattt tcaccgatgg
     1141 ttcgtgtctg ggtaatcctg gacctggggg ttacggcgct attttacgct atcgcggacg
     1201 cgagaaaacc tttagcgcag gctacacccg caccaccaac aaccgtatgg agttgatggc
     1261 cgctattgtc gcgctggagg cgttaaaaga acattgcgaa gtcattttga gtaccgacag
     1321 ccagtatgtc cgccagggta tcacccagtg gatccataac tggaaaaaac gtggctggaa
     1381 aacagcagac aaaaaaccag ttaaaaatgt cgatctctgg caacgtcttg atgctgcgct
     1441 ggggcagcat caaatcaaat gggaatgggt taaaggccat gccggacacc cggaaaacga
     1501 acgctgtgat gaactggctc gtgccgcggc gatgaatccc acactggaag atacgggcta
     1561 ccaagtcgaa gtttaagcct gtggtttacg acattgccgg gtggctccaa ccgcctggcg
     1621 aattcgtggc ttgtttttac tctgtttcat cggatttagc gttaaaggaa tagtccgttt
     1681 ccgggcaaca ataagttgta agcaaccaag cgcaggaata tgcgcattca aaagttttcc
     1741 tccgtgtttg ttccatggga gaacgtggaa acggctggcg tgtagcactt caaaattcaa
     1801 caaagagagc cagtccagtt gccgcatcag agtaaacatc cggctgttat agggcgaggt
     1861 tttgcgcaat accggcacaa gtttgcgtaa tcccattaaa ctaatgggat tgaagccact
     1921 aatgaccagc cagccatcat caatcaatac ccgatcggct tcacgcaata aacgatgcgg
     1981 atcggtgcac cacggcaatg tatgtgccag tagacaaaca tcaacggatt tatcggcaaa
     2041 aggaagatga agtgggtccg cctgcacctg gacaggcatt ccttgcgcag aaacattcac
     2101 ttgatgagaa accgcgcacg cttcgcaatt gatttctgcg cttaaattgc caatcttaag
     2161 cagatgaaaa ccatacattt tagtgaacca cgggttgagc tggcgctcca gcgccttgcg
     2221 ataaagctct ccccagggca aatcgcccca gcgatcagga gccacgacag tttgagggac
     2281 tcttgccggt ttcatcacaa ccttccgttt cacactgaga ggtaatctat gaatcttaac
     2341 agtattcccg cctttgatga caattacatc tgggttttga atgatgaagc aggtcgctgc
     2401 ctgattgtcg atcccggaga cgcagagcca gtattaaacg ccattgccgc caataactgg
     2461 caaccggagg ccatatttct cacccaccat catcacgatc acgttggcgg cgtaaaagaa
     2521 ctggtggaaa agtttccgca aattgtggtg tatggtccac aagagacaca agataaggga
     2581 acaacacagg tagtcaaaga tggcgaaact gccttcgttt tagggcatga atttagtgta
     2641 attgccacac cgggtcacac tttaggacat atctgttact tcagtaaacc ttatctattt
     2701 tgcggcgaca cgctgttttc tggtgggtgt ggtcggttgt ttgaagggac accatcacaa
     2761 atgtatcaat cacttaaaaa gttaagtgcg ttacctgacg atacattggt atgttgtgct
     2821 catgaatata ccttatcaaa tatgaagttt gctttgagta ttcttccgca cgatttgtcc
     2881 ataaatgatt attatcgtaa agttaaggag ttacgggcaa aaaatcaaat aacactaccc
     2941 gtaattctga aaaatgagcg gcaaattaat gtttttttaa gaacggaaga tattgattta
     3001 attaatgtaa ttaatgaaga aacattattg caacaacctg aagagcgttt tgcatggtta
     3061 aggtcaaaga aagataggtt ctgataaaac tttcttgtca tcggctccgt tcgccgttat
     3121 gatcggtcgt cttttaagca actattgaca cacacatgaa ggcaaaagcg atattactcg
     3181 cctctgtcct gctcgtgggt tgccagagta ccggcaacgt tcaacagcac gcacagagcc
     3241 tttctgcagc tggtcaaggg gaagcagcaa agtttacaag tcaggcacga tggatggacg
     3301 atgggacgtc tatcgcgcca gatggtgact tgtgggcttt cattggcgac gagctaaaga
     3361 tgggaattcc ggaaaatgac cggattcgcg aacagaaaca gaaatattta cgcaataaga
     3421 gctatctcca cgatgtaact ttacgggcag agccgtatat gtactggata gccgggcaag
     3481 ttaaaaaacg taacatgcct atggaactgg tactactacc catagtggag agcgcttttg
     3541 atcctcacgc aacgtctggc gccaatgccg caggcatctg gcagatcatt ccgagcacgg
     3601 ggcgcaatta tggtttgaaa cagacccgca attatgacgc gcgtcgcgat gttgttgctt
     3661 caacaactgc cgcgctgaat atgatgcagc gtctgaacaa aatgtttgat ggcgactggc
     3721 ttctgaccgt agcggcttat aacagcggcg aaggtcgggt catgaaggca attaaaacga
     3781 acaaagcgcg tgggaaatcc acggacttct ggtcgttacc gttgccgcag gaaacgaagc
     3841 agtacgtgcc taaaatgctg gcattgagtg atattctcaa aaacagcaag cgttatggcg
     3901 tacgtctgcc aacgaccgat gaaagccgtg ctctggcgcg tgtgcacctg agtagcccgg
     3961 ttgaaatggc gaaggttgca gatatggcgg ggatttccgt cagcaagctg aagacattca
     4021 acgctggcgt gaaaggctcc acgctgggcg caagtggtcc gcaatacgtg atggtgccaa
     4081 agaagcatgc agatcaactg cgtgaatctc tggcttcagg cgaaattgct gctgtacagt
     4141 cgacgctggt tgccgacaat acgctgctta acagccgtgt ttacaccgta cgctctggcg
     4201 acacgctttc aagtatcgct tcacgtctcg gcgtaagcac caaagatttg cagcagtgga
     4261 acaaactgcg cggatctaag ctgaagccag gccaaagttt gacgattggt gcaggcagta
     4321 gcgcacagcg actggcaaac aacagcgata gcattacgta tcgtgtgcgc aaaggcgatt
     4381 cgctttcaag cattgctaaa cgccacggcg tgaacatcaa agatgtgatg cgctggaaca
     4441 gcgacactgc gaatctgcaa ccaggcgata agctgacgtt gtttgtgaaa aacaacaaca
     4501 tgccagattc ctgacaaacc agataataaa aaggcaccga ttcccccggt gcctttttta
     4561 tttatgccgc tttatgtgca tctaccatga tgatatcact ggtgaaagag ccatcattct
     4621 gcaaggcaaa atacgttctc acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt
     4681 ctactaacgc ttctggcgta cgcattctcg cgacccatga agaaaattcc agcggtaact
     4741 tatctgtaat taaattatca actatcagat tggcttcatt gattaacctc aaccactcac
     4801 cgctggcgta gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgtc tgtaaccaga
     4861 tgtcgcgcac tgggtgaccc ggagacatta cgtccatcac aatcagccta ccgccaggtt
     4921 tcaatatccg gttcacttca cgcagtgctg caccaacatc atgccagtga tgggcagaat
     4981 aacggctgat aacaatatca aatgcgttat cggcaaatgg cagactttcg gcataaccct
     5041 ggcgggtggt gatatttttc agttgccgga cttcggcagc ttgtgccacg acatccagca
     5101 tttgggcaga taagtcatac gccaccaccg tgctcacgtt ttgcgcagcg acaaagctgg
     5161 catgccctgc tccgcagccc atatcaagta cacttgcatc aggataatca gccagacaca
     5221 ccgccaggcg ctgtaaatct cgtccggatg catgtacggt actggtcaga tattcgctgg
     5281 cttgagagct aaactgcttt tctacatggt catggtggga ttgtattgtc attgcgctgt
     5341 ccttttgtta tttgagtata aaagggcagc gccaaacgtt ggtctgccct gtggcagacc
     5401 tgacatacct tatttatcag gcttgccggg actgaattca acgagtagcg gattgtgatc
     5461 ggacgcgcgc gtaaccagta cagaagcttc gctgacgttc agaccacggt agaaaacaaa
     5521 atcgagcggg cgaccaaacg cccgacggcg ctgatcatcg gtaaaacgca cctgacgcag
     5581 cgacatttcc cgcgcaaagc gatataacgc gttcatcctt ctacggctcc aggcattgaa
     5641 atctcccgcc ataatgaccg ggccgctgtg gtgagctatc tgatcaccaa taggaagtaa
     5701 ctgcttacta tagacatcca cgcccagact gaagttgacg gcgtgtatat taaccaccat
     5761 caacaggcgg gtgtcaggta atggatagac cgtcaccagt gccgacttcg ccagacgcaa
     5821 aatgggctct cgttcacgta acgggcagca atacactgga tgcgccgccg aaagggtcat
     5881 tacgccagaa ggatgttgtg gcagcacgaa agcgggtacc tgatcggcgg caagatagtt
     5941 agcggtcgca aactgtacta actctggcgt tgtctgcgct tcctgcaata acaccagatg
     6001 tgcatctttg ccgtagttct ttaataccga caaccattca gcgcgttgct gtttgtatat
     6061 gttccacacc aggatccgaa tacgctcttc ggtacttaac ggttctccgg gtggtaatgc
     6121 ctggccgatg ctcgcaaaag accccggcgg taagatcctt tccgcaggtt gtccggcaac
     6181 atagcgcatg gcataggtgt tttttcgcac ttttgacttt gaaacctcta ttacgtcaac
     6241 ccttccgcac tcgggagggt tctactgtta tagggatttt agctcactct ttcaacgagc
     6301 aattgtatat tgttatgtaa gcaagtgctt acaccggtca gaattttgct cattattgag
     6361 aacattaaat cggatgacat atcacgcatc ctgatggttt aatgacaaat aaattcaaaa
     6421 tatctcttct attttgcaac aggagcaaaa atgaaagcca cgtcggaaga actcgccatt
     6481 tttgtttcgg tcgtagaaag cggcagcttt agccgggcag cggaacaatt agggcaagca
     6541 aactcagcgg taagccgggc ggtgaaaaag ctggagatga aacttggcgt tagcctgctt
     6601 aatcggacca cgcgacaact tagcctgacg gaagaaggcg agcgttattt ccgtcgcgta
     6661 cagtcaattt tgcaggagat ggcagcggca gaatcagaaa ttatggagac gcgtaataca
     6721 ccgcgtggac tgttacggat cgatgccgca actccagtgg tgctgcactt tctgatgccg
     6781 ttaattaagc ctttccgtga acgctatccg gaagtcactt tatcgctagt ctcctccgaa
     6841 acgattatta atttgatcga aagaaaagtg gatgtcgcga tacgcgccgg tacgttaacg
     6901 gattccagct tacgtgccag gccgttattt aacagttatc gaaaaattat cgcctccccc
     6961 gattatattt cccgctacgg gaagccagaa acgatcgacg atttaaagca acatgtttgc
     7021 ctgggattca ctgaacccgc ttccctcaat acctggccga tagcctgtag tgatggacaa
     7081 ttacatgagg tgaagtacgg tttgtcatcc aatagtgggg aaacactgaa acagctttgc
     7141 ctgagtggca acgggattgc gtgtttgtcc gactacatga tcgacagaga aatcgctcgc
     7201 ggagaattgg tggagttaat ggcagataaa gtgttgccag tggaaatgcc attcagtgcc
     7261 gtctattata gcgaccgtgc ggtaagtacg cgcatccggg cttttatcga tttccttagc
     7321 gagcatgtaa aaacagctcc cggaggagct gtcagagagg cttaatccca ttcaggagcc
     7381 agaccttccg ggctaaccag gcggtcgttg caatccagtg cggcgatcgc ttttttatct
     7441 tcggcatcaa gctgtaaatt ttgtgcctta agattactct ccaggttttc acgtttagta
     7501 gaagaaggaa ttactgagta accttccccc atagcccacg ccagaatcac ttgtgccgga
     7561 gtcgcattgt gtttagctgc gatacgagca ataacctcat ctttcagggc cttaccatac
     7621 gccagcgtca tataggaagt aatatggatg ccgtgctgtt tagcccaggc aaccacttta
     7681 cggttttgca gataaggaga gagttcaatc tggttagtag cgatgttttc agcgccaaca
     7741 gcagcaatcg ccttttccat caatgggatc gtgaagttgg aaataccgat ctcacgcgtc
     7801 aacccttctt ttttggcttc cagcagcgcc tgcataaact cttcaacaga gacttcatcg
     7861 tttggtgacg gccagtggat tagcgtcaga tcaacataat cagtacgcaa tttttgcagg
     7921 ctctctttca gacttgggat caatttgtct ttgccgagat tttcaatcca gattttagtg
     7981 gtgatgtaga gttcatgacg tggcacgcca ctttctgcaa tcgcctgacc tactgcggct
     8041 tcgttatcat agatttgtgc ggtatcaatt gcgcgataac caagttcaag cgccgttttc
     8101 acagatgaaa taacaacgtc gtctttcaga cggaaagtac ctaaaccaaa tgcagggata
     8161 gccataatat gcctctttta atgctatttt cgttaactga ggtgattatg ggcttcagac
     8221 tgttgaggaa aaagagcgta aaatgcagag gatttttgcg attctggcaa taatagatat
     8281 acagacgaaa aaaaacctcg agcgttaact cgaggcttct taata
//
LOCUS       DAZFFG010000168         8298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFFG010000168 DAZFFG010000000
VERSION     DAZFFG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.169"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..299)
                     /locus_tag="ACHJ7J_004520"
     CDS             complement(<1..299)
                     /locus_tag="ACHJ7J_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5002650.1"
                     /translation="MMPFARYFCIFINVGLGEAAKRDVGTGDNQIPDMGAFASGSGWF
                     RLPGGYIVQFGTFSGNTTRFISGHFPIPFPNQPMVSVSVMSDAVQSDPSNPAPQV"
     gene            326..418
                     /locus_tag="ACHJ7J_004521"
                     /pseudo
     CDS             326..418
                     /locus_tag="ACHJ7J_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            539..781
                     /locus_tag="ACHJ7J_004522"
                     /pseudo
     CDS             539..781
                     /locus_tag="ACHJ7J_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="catalyzes the release of sulfite from
                     alkanesulfonates; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkanesulfonate monooxygenase"
     gene            793..1584
                     /gene="ssuC"
                     /locus_tag="ACHJ7J_004523"
     CDS             793..1584
                     /gene="ssuC"
                     /locus_tag="ACHJ7J_004523"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO5002651.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            1581..2348
                     /gene="ssuB"
                     /locus_tag="ACHJ7J_004524"
     CDS             1581..2348
                     /gene="ssuB"
                     /locus_tag="ACHJ7J_004524"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5002652.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYTENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIYNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(2391..5003)
                     /gene="pepN"
                     /locus_tag="ACHJ7J_004525"
     CDS             complement(2391..5003)
                     /gene="pepN"
                     /locus_tag="ACHJ7J_004525"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO5002653.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELSDELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            5269..6471
                     /gene="pncB"
                     /locus_tag="ACHJ7J_004526"
     CDS             5269..6471
                     /gene="pncB"
                     /locus_tag="ACHJ7J_004526"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO5002654.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQEAIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            6640..8040
                     /gene="asnS"
                     /locus_tag="ACHJ7J_004527"
     CDS             6640..8040
                     /gene="asnS"
                     /locus_tag="ACHJ7J_004527"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO5002655.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         1874 a         2106 c         2222 g         2096 t
ORIGIN      
        1 aaaacctgcg gggcaggatt cgacgggtct gactgaacgg catcagacat aacactgact
       61 gaaaccatcg gctgattagg gaatggtata gggaagtgtc cactgataaa gcgggtcgtg
      121 tttcctgaaa aagtgccaaa ctgaacaata tatccacctg gtagcctgaa ccatcccgaa
      181 ccagaagcga atgctcccat atccggtatc tgattatctc ctgtgcccac atcccttttc
      241 gccgcttctc ccaaaccaac atttatgaaa atgcagaaat aacgagcaaa tggcatcatc
      301 cctgcttttg tcagggtgat ctaccatgtt tataggctat gtacgcgcat caacaaatga
      361 ccaggacacc gatttacaac gcaatgcgtt gaactgtgca ggatatgagc tgattttttg
      421 agggtaaatc atcagcatga gactaataat ttagcgtcca attctgggct ttttggttgc
      481 gctctctccg cccagaattg tgcgccaatt agcccagaaa aaaacgcgaa gttacaccgg
      541 gacggcgctg gtgggcgatg gtcctacggt cgctgcgcga atcaacgaat atgccgcgct
      601 tggcatcgac agctttgtgc tttcgggcta tccgcatctg gaagaagcgt atcgggtcgg
      661 cgagttgctg ttcccacatc tggatgtcgc cattccggaa attccccagc cgcagccgct
      721 gaatccgcaa ggcgaagcgg tggcgaatga ttttatcccc cgtaaagtcg cgcaaagcta
      781 aggaacacga tgatggcaac gccagtgaag aagtggttat tgcgcgttgc cccctggttt
      841 ttaccggtgg gcatcgtggc ggtgtggcaa ctggcctcct cagttggctg gctgtcgacg
      901 cgtattttgc cttcaccgga aggagtggtg acggcgttct ggacgctctc cgccagcggc
      961 gaactgtggc agcatctggc gatcagctcc tggcgggcgc tgattggctt ttcaattggc
     1021 ggatcgctgg ggttgatttt gggactgatt agcgggctat ctcgctgggg agaacggctg
     1081 ctggatacct caattcagat gttgcgcaac gtgccgcatc tggcgctgat cccgctggtg
     1141 attttgtggt ttggcattga tgaatccgca aaaatctttc tggtggcgct cggtacgcta
     1201 ttccccattt atatcaacac ctggcatggc atccgtaata tcgatcgcgg gttggtggag
     1261 atggcgcgta gctatggatt atccggcata ccgctgttta tccatgtgat cctgcctggt
     1321 gccctgccct caattatggt cggcgtgcgt tttgcgttgg gcctgatgtg gctgacgctg
     1381 attgttgccg aaaccatttc tgccaattca ggcattggtt atctggcgat gaatgcgcgg
     1441 gagtttttgc aaacggacgt ggtggtggtc gccattattc tttacgccct gctcggcaaa
     1501 ctggctgacg tcagcgcgca gctgttagag cgcctctggt tgcgctggaa cccggcttat
     1561 catttgaagg aggccacggt atgaatactg ctcgtctgaa ccagggcacg ccattgttgc
     1621 tcaatgcagt aagcaaacat tacacggaaa atatcgtcct gaaccaactg gatttgcata
     1681 ttccggcagg tcagtttgtg gcggtggtgg gccgcagcgg tggtggcaaa agtaccctgc
     1741 tgcgcctgct ggcaggtctg gaaacgccaa ccgctggcga tgtgttagcg ggcaccacac
     1801 cgctggctga aattcaggaa gacacgcgaa tgatgtttca ggacgcgcgt ctgctgccgt
     1861 ggaaatcggt gatttataac gttgggttag gtcttaaagg ccagtggcgc gatgccgcgc
     1921 gtcgggcgct ggctgcggta gggctggaga accgcgccgg ggaatggcct gccgcacttt
     1981 ctggcgggca gaaacagcgc gtggcattgg caagggcatt aatccatcga ccggggttat
     2041 tgttgcttga tgaaccactc ggtgcgctgg acgccttaac gcggctggaa atgcaggatt
     2101 tgattgtgtc gctttggcag gagcatggtt ttaccgtctt gctggtgacg catgatgtga
     2161 gcgaagcggt agctatggct gaccgggtgc tgttaattga agagggaaaa attggtctgg
     2221 atttgaccgt ggatattcct cgtccgcgcc ggttaggatc ggtacggtta gcagaacttg
     2281 aagcggaagt gttgcaacgg gtgatgcagc gtggtgaatc tgaaactcgc ctgagaaaac
     2341 aggggtaaat tccccgaatg gcggcgctat tgccgccatt cggttattta tcaagccagt
     2401 gctttagtta tcttctcgta cagatcgcca gagagatttt ccagcccttt caactgttcc
     2461 agcgccgcgc gcattttctc ctgacgtttg gcatcgtaac gtttcaggcg aatcagcggt
     2521 tcaatcagac gtgaagccac ctgcgggtta cggctgttga ggtcggtaag catttccacc
     2581 aggaactggt aaccgctccc atcttcagca tggaacgctg ccgggttgct gcccgcaaac
     2641 gcgccaatca acgaacgaat acggttcggg ttgctcatgg taaatgagcg atgctgcaac
     2701 aaaccacgca ccgtctccag cacattcgcc gccgggctgg tggcttgcag gataaaccat
     2761 ttatccatca ccagaccatc ctgatgccac ttgtcgtcgt actcctgcat cagcgcgtca
     2821 cggcaaggca gctgtgcagc aaccgccgca gaaagcgctg ccagcgcatc ggtcatattg
     2881 tttgcttcat ggtattgctt gctcaccagc acgtcagcca gatgcgtttc accaaaagca
     2941 aggaagcgca ggcaggcatt acgcagagtg cgttttgcaa tatcttcatg ctcaacacgg
     3001 tactcactct ggtaattcgc gttgtaaata gccagcagct catccgacag ttcagtcgcc
     3061 agagtacgag tgagtgcttc gcgtacttcg gcaatagcaa tcgggtcgat gatatcgaac
     3121 agttcagcca tttcattgac agaaggcagc gtcaggattt ctgccgccag cgccggatca
     3181 atcttctcat cgagcagcac cgcgcggaaa gcgtcagcca catgtaccgg cagagacagc
     3241 ggctgccctt gctgatgacg ggcgacgttc agcttgatgt aggttgccag caaactttgc
     3301 gccgcatccc aacgggagaa atcattacgc gcatgacgca tcaggaaggt cagttgctga
     3361 tcgctccact tatattccag tttcactggc gcagagaatt cgcacagcag cgcaggcacc
     3421 ggctggaagt agacattatc aaaaacaaag gtctgttccg cctgggtgac gttcagcacg
     3481 gaattcaccg gatgaccgcc tttctgcaac gggatcactt tgccttcgtt gtcatacaat
     3541 tcgatggcaa acggaatatg cagcggctgt ttttctgcct gatccggcgt ggctggcgtg
     3601 cgctggctga tggtcagggt gtactgctcg gtttccggat tgtagtcgtc tttgacggtc
     3661 acaaccggcg taccggactg gctgtaccaa cggcggaaat gggagagatc gacattcgac
     3721 gcatcttcca tcgcctgcac aaaatcgtca caggtcgctg cgctaccatc atgacgctcg
     3781 aaatagagct gcatcccttt ctggaaattt tcttcgccaa gcagggtgtg gatcatgcga
     3841 atcacctccg cgcccttctc gtaaacggtc agggtgtaga agttgttcat ctcaatgacc
     3901 atatccgggc ggattgggtg cgccatcggg ctggcgtctt ccgcaaactg caatccgcgc
     3961 atggtgcgca cattattgat acggtttacc gcgcgggaac caaggtcaga gctgaattcc
     4021 tgatcgcgga agacggttaa accttctttc aggctgagct ggaaccagtc gcggcaggtc
     4081 actcggttac cggtccagtt atggaaatat tcatggccga taacgcgttc aatatcgagg
     4141 taatctttgt cggtggcggt atcggtgcgg gccagcacat atttggagtt aaagatattc
     4201 agccccttat tctccattgc gcccatattg aagaaatcca ccgcgacgat catatagatg
     4261 tcgaggtcat actccaggcc gaagcgttct tcatcccatt tcattgagtt tttcagcgag
     4321 gtcatcgccc acggcgcgcg atcaaggtta ccgcgatcga cgtacagctc cagcgctact
     4381 tcgcgaccag aacgcgtggt gaaggtgtcg cgcagtacat caaagtcgcc tgccaccagc
     4441 gcaaacaggt agcacggttt cgggaacggg tcctgccact gtacccaatg acgtccgttt
     4501 tccagttcgc cttgcgcaac gcggttaccg ttggaaagca ggaaaggata tttggtttta
     4561 tcggcaataa ttttggtggt aaaacgcgcc agcacgtccg ggcggtcgag ataatacgta
     4621 atatggcgga aaccttcggc ttcacactgg gtgcaaagcg catcgcctga ctgataaagc
     4681 ccttccagcg ccgtattcgc cgccgggctt atttcattaa tgatcttaag cgtaaaacgc
     4741 tccggcaaat tactgatgac cagtgcgccc tcttcttctt tccaggcggt ccacggctca
     4801 tcattaatat gaacagaaac cagtttgagg tcttcgccgt tgagacgaag cggagcatct
     4861 gatgcaccat gacggacagc ctggctgacc gcggtaacga ccgtcttttg cgcgtcgagg
     4921 tcaaaggtca agtcaatatc agtaatctgg taatccggcg cacgataatc gtgacggtat
     4981 ttggcttgtg gctgttgagt cataaataac ctttagcatc ttttatagag tctggtgttc
     5041 agtctattcc tgttgcgtaa atcgcgctat gcagaatctt catcttttca ggtacaaacg
     5101 cctttattgc tacattttta taacatacac cgcgtaatgc catcgaccag aaaggtggca
     5161 tatggtgtga tcggggttca ataaattgcg aaacaaggta tactccagca gttcctgaag
     5221 atgtttattg tactaaacgc tcctgtacga ggacgctact gcgcacctat gacacaattc
     5281 gcttctcctg ttctgcactc gttgctggat acagatgctt ataagttgca tatgcagcaa
     5341 gccgtgtttc atcactatta cgatgtgcat gtcgcggcgg agtttcgttg ccgaggtgac
     5401 gatctgctgg gtatttatgc cgatgctatt cgtgaacagg ttcaggcgat gcagcacctg
     5461 cgcctgcagg atgatgaata tcagtggctt tctgccctgc ctttctttaa ggccgactat
     5521 cttaactggc tacgcgagtt ccgctttaac ccggaacaag tcaccgtgtc caacgataat
     5581 ggcaagctgg atattcgttt aagcggcccg tggcgtgaag tcatcctctg ggaagttcct
     5641 ttgctggcgg ttatcagtga aatggtacat cgctatcgct caccgcaggc cgacgttgcg
     5701 caagccctcg acacgctgga aagcaaatta gtcgacttct cggcgttaac cgccggtctt
     5761 gatatgtcgc gcttccatct gatggatttt ggcacccgtc gccgtttttc tcgcgaagta
     5821 caagaagcca tcgttaagcg tctgcaacag gaatcctggt tcgtgggcac cagcaactac
     5881 gatctggcgc gtcggctttc cctcacgccg atgggaacac aggcacacga atggttccag
     5941 gcacatcagc aaatcagccc ggatctagcc aacagccagc gagctgcact tgctgcctgg
     6001 ctggaagagt atcccgacca acttggcatt gcattaaccg actgcatcac tatggatgct
     6061 ttcctgcgtg atttcggtgt cgagttcgca agtcggtatc agggcctgcg tcatgactct
     6121 ggcgacccgg ttgaatgggg tgaaaaagcc attgcacatt atgaaaagct gggaattgat
     6181 ccacagagta aaacgctggt tttctctgac aatctggatt tacgcaaagc ggttgagcta
     6241 taccgccact tctcttcccg cgtgcaatta agttttggta ttgggactcg cctgacctgc
     6301 gatattcccc aggtaaaacc cctgaatatt gtgattaagt tggtagagtg taacggtaaa
     6361 ccggtggcga aactttctga cagccctggc aaaactatct gccacgataa agcgtttgtt
     6421 cgggcgctgc gcaaagcgtt cgaccttccg catattaaaa aagccagtta atatcatcag
     6481 ggagctaatc ggctcccttt tttaccttta attccgaaat ctttcgctgc atttgcgaat
     6541 tctgcttgtc tgattgcaga tgccaggtaa cataggtatc ccccccattt acgggtggac
     6601 aagtgtttat tttttccgac tattaacaga gagaatatta tgagcgttgt gcctgtagcc
     6661 gacgtactcc agggccgcgt agccgttgac agcgaagtca ccgtacgcgg atgggtacgt
     6721 acccgccgag attcaaaagc tggcatctcc ttcctcgccg tttatgacgg ttcctgcttt
     6781 gatcctgtac aggctgtcat caataattct ctgcccaatt acaatgaaga cgtcctgcgt
     6841 ctgaccaccg gttgttcggt cattgtgacg ggtaaagtcg tggcgtcgcc gggccagggg
     6901 caacaatttg aaattcaggc cagcaaggtt gaagttgctg gttgggttga agatccagac
     6961 acgtacccga tggcggcaaa acgccacagc attgagtatc tgcgtgaagt cgctcacctg
     7021 cgtccgcgca caaacctgat tggtgccgtc gcgcgcgttc gtcatacgct ggcgcaggcg
     7081 ctgcatcgct tctttaacga gcagggattc ttctgggttt caacgccact gattaccgca
     7141 tctgataccg aaggtgcagg cgaaatgttc cgcgtttcta cgctggatct ggaaaacctg
     7201 ccgcgtaacg atcagggcaa agtggatttc gacaaagact tctttggtaa agagtctttc
     7261 ctgaccgtat ctggccagtt gaacggcgaa acctacgctt gcgcattgtc caaaatttat
     7321 actttcggcc cgactttccg tgctgaaaac tccaacacca gccgtcacct ggcggaattc
     7381 tggatgctgg agccggaagt ggcgtttgct aacctgaacg atatcgcggg tctggctgaa
     7441 gccatgctga aatatgtctt caaagcggtt ctcgaagaac gcgcagacga catgaaattc
     7501 ttcgctgaac gcgtagataa agatgccgtt tcacgtctgg aacgcttcat tgaggccgat
     7561 ttcgcgcagg tggattacac cgacgcagtg accattctcg aaaactgcgg caggaagttt
     7621 gaaaacccgg tttactgggg cgtcgatctc tcttctgagc atgagcgtta tctggcggaa
     7681 gaacacttta aagcaccggt agtggttaaa aactatccga aagatattaa agcgttctat
     7741 atgcgcctca acgaagacgg taaaaccgtt gcggctatgg acgttctggc tccgggcatc
     7801 ggtgagatca ttggtggctc ccagcgtgaa gaacgtctgg acgtgctgga cgagcgtatg
     7861 ctggaaatgg gcctgaataa agaagattac tggtggtatc gcgatctgcg tcgctacggt
     7921 actgttccgc attcaggttt cggtcttggt tttgaacgcc tgattgctta cgtaactggt
     7981 gtgcaaaacg tgcgtgatgt gattccgttc ccacgtactc cgcgtaacgc cagcttctaa
     8041 tttagcgtct acaagagcca gcacccgctg gctctttttt atcctttctg tcaagttatc
     8101 tgtttgttaa gtcaagcaat ctatttgcaa ccccgccata acgatcatcc tgttacggaa
     8161 tattacattg caacatttac gcacaaaaaa taacccgcat tcttattgcg gattagtttt
     8221 ttcttagctg atagcacaat tttcatacta ttttttggca ttctggatgt ctgaaagagg
     8281 attttgtgcc aggtcgat
//
LOCUS       DAZFFG010000169         8298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFFG010000169 DAZFFG010000000
VERSION     DAZFFG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.170"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            331..846
                     /gene="zur"
                     /locus_tag="ACHJ7J_004528"
     CDS             331..846
                     /gene="zur"
                     /locus_tag="ACHJ7J_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO5002656.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(888..1097)
                     /locus_tag="ACHJ7J_004529"
     CDS             complement(888..1097)
                     /locus_tag="ACHJ7J_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO5002657.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(1213..2538)
                     /gene="dinF"
                     /locus_tag="ACHJ7J_004530"
     CDS             complement(1213..2538)
                     /gene="dinF"
                     /locus_tag="ACHJ7J_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO5002658.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKAAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFTAT"
     gene            complement(2611..3219)
                     /gene="lexA"
                     /locus_tag="ACHJ7J_004531"
     CDS             complement(2611..3219)
                     /gene="lexA"
                     /locus_tag="ACHJ7J_004531"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO5002659.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(3329..3697)
                     /gene="dgkA"
                     /locus_tag="ACHJ7J_004532"
     CDS             complement(3329..3697)
                     /gene="dgkA"
                     /locus_tag="ACHJ7J_004532"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5002660.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            3868..6291
                     /gene="plsB"
                     /locus_tag="ACHJ7J_004533"
     CDS             3868..6291
                     /gene="plsB"
                     /locus_tag="ACHJ7J_004533"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO5002661.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(6446..7318)
                     /gene="ubiA"
                     /locus_tag="ACHJ7J_004534"
     CDS             complement(6446..7318)
                     /gene="ubiA"
                     /locus_tag="ACHJ7J_004534"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO5002662.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(7330..7827)
                     /gene="ubiC"
                     /locus_tag="ACHJ7J_004535"
     CDS             complement(7330..7827)
                     /gene="ubiC"
                     /locus_tag="ACHJ7J_004535"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO5002663.1"
                     /translation="MSHPALTQLRALRYFTEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(8050..>8298)
                     /locus_tag="ACHJ7J_004536"
     CDS             complement(8050..>8298)
                     /locus_tag="ACHJ7J_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002664.1"
                     /translation="ILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEIL
                     PVNLTPETLFGSKSQDKHALFPIFTASWRATRIKNKGI"
BASE COUNT         2122 a         2268 c         2030 g         1878 t
ORIGIN      
        1 tatcttcaac atgatttatc tacctttttt gtcgtaccgt gtcctcctta taagtgtttt
       61 gccatctggt cactatcaaa aatcgctcat aattttctga actacaggta tgcgatcaac
      121 cccagttaaa taaatagcga gttaacttga agcaatttat ttaaaaatga attattaatt
      181 attatcgtgt tgataatggt acgaccaggc aaaacaacac cttacgtaca aaacagaaaa
      241 tcaacattac ggcaacatta agggttctcg gtgtttttcc tggaacatgg taaagtaagg
      301 acattcttaa cccccacttt gaggtgcccg atggaaaaga ccacaacgca ggagttatta
      361 gcgcaggctg aaaaaatctg cgcgcagcgt aatgtgcgcc tgaccccaca gcgcctggaa
      421 gtgttgcgcc tgatgagtct gcaggatggc gctatcagcg cttatgatct gcttgatttg
      481 ctgcgcgaag ctgaaccgca agccaagccg ccaacggttt atcgcgcgct ggattttctg
      541 cttgagcaag gttttgtgca taaggtggaa tccaccaaca gttatgtgct ctgccatctg
      601 ttcgaccagc ccacccatac gtcagctatg tttatttgcg atcgctgcgg cgcggtgaaa
      661 gaagaatgtg cagaaggcgt ggaagacatc atgcatacgc tggcggcaaa aatggggttt
      721 gccctgcggc ataatgtgat tgaagcacat gggctctgtg cggcatgcgt ggaagtggaa
      781 gcgtgtcgtc atcctgaaca gtgccagcat gatcactctg tgcaggtgaa aaagaaaccg
      841 cgttaagagg gcgtacatcc ttgtacacgt cgggcaggag ggattaatta ccagcgatat
      901 tcattgcggg tttcccaatc cacgacctct ttttctgcct gatctttctg ataaccataa
      961 cgttcctgga ttttaccgac cagttgatca cgtttacctt caatgatcgt catatcatca
     1021 tcggtcagtt tgccccattg ctctttcact ttacctttaa actgtttcca gttaccgccg
     1081 gcttcatctt tattcataat caagacctca tcgttaggtt gtgaatgaga gtacgttcac
     1141 ttttcttctg aacgtgagat taagtgtagt cagcaatttg gcttaggatt atttattcag
     1201 aatttttaac cgtcacgttg cggtaaacca ggtaccattg cgccagtgac gccgccagat
     1261 agccgccaga gaaagcccgc gcaacgccag aaagacggtt aacgccaacc acaaaccatg
     1321 attacccagc cacggcagcg taaggagcgt cagcgcaaaa cctgcggcgg ccaccgccat
     1381 actgttacgc atttcggcgg cacgcgttgc gcctataaac atgccgtcca gcagataaca
     1441 ccagacgccg accaacggca aaatcacctg ccagataaga tagcggtcgg ccagctgctg
     1501 aatctgggtt aacgacgtca gcaacgcaat gatgtgttcc cctgccagca aataaaccac
     1561 cgaaaacagt aacgctacga tccccgactg gcggcacgct gcccgccaga catccagcaa
     1621 ctggctaccg tcgcgcgcac cgtatgcctg accggaatgt gcttcaaccg cgtaggcaaa
     1681 accatccagc gcataggcgg taaaggtgag tagcgtcatc agaaccgcgt ttacagcgat
     1741 aatgtcactc cccagtcgcg caccaagtac ggtgatcgcg ccgaaacaga gttgcaacaa
     1801 cagcgagcgc agcatgatat cgcggttaag cgccagcaag cgacggaagt ttcctcgcca
     1861 ggcagctttc agcatttcgc cggagattcc gcgtagtttg aggatcttac gcaccattag
     1921 cagaccaatc agcaatgttg catattccgc aataaccgtc gccagcgccg caccctgcac
     1981 gttcatatgc agccccatta ccagccagac atccagcaca atgttgagga tattgccgac
     2041 cactaacaaa attactggcg cacgggcata ttgcacgccg agtaaccaac caagtaatac
     2101 cagattcgcc agcgacgccg gcgcgcttaa ccagcggatt tcaagaaagc gccgcgcctg
     2161 ttccaggact gcttcactac cgccaacaat atgcagcgcc agatcgataa tcggcgtacg
     2221 cagcagcgca attaacgccc cagcccccaa cgccaacagc aacggttgca ccagcgcacg
     2281 ggctaatgcc tgaggatttt tggcaccata agcctgcgca gtcagcccgg tggtgctcat
     2341 gcgtaaaaac agcaacaaca taaagagaaa gctggtcgcc gttgcaccaa ccgccacgcc
     2401 gcccaaataa accggactat caagatgacc aataaccgcc gtatcgacca gacccagcaa
     2461 cggaacggtg atattggaga aaatcatggg taaggcgaga tgccagagtg ctttatcaga
     2521 tgaagtgagg aatgccatgc agacaagcct gatgaagaga gatgaaaaac aaaccgcgac
     2581 gccaggcggc atcgcggtct cagagatatg ttacagccag tcgccgttgc gaataacgcc
     2641 aaccgccagc ccttcaatgg tgaagctctg ctgacgaagg tcaacgacaa ttggtttaaa
     2701 ctcgctattt tctggcaaca gttcgacttt attgccctgt tttttcaggc gcttaacggt
     2761 aacttcgtca tcaatacgtg cgacaacgac ctgaccgtta cgtacatcct gagttttatg
     2821 cactgccagc aagtcgccat ccataatgcc gatatctttc atcgacatcc cgctgacgcg
     2881 cagcaggaaa tcagcattcg gcttgaacaa ggaaggatcg acctgataat gaccttcaat
     2941 atgctgctgc gccagaagcg gttcaccggc agccacacga cctaccagtg gcaacccttc
     3001 ttcctcttcc tgcaacagac gaatcccgcg tgatgcgccg gaaacaattt caattacgcc
     3061 tttgcgtgcc agcgccttca gatgttcttc agccgcgttt ggggaacgga accccaaacg
     3121 ctgcgcgatt tccgcacgcg tcggcggcat acctgtctgg ctgatgtgat cacggatgag
     3181 atcaaacacc tcttgttgcc tggccgttaa cgctttcatt ccgccccctg ggtgtatata
     3241 cagttatgct gtgagtatat acagcaaaag gcgattttgg aaccataaac tgcacaataa
     3301 accagagatt tatcgaattc tggaagggtt atccaaaatg cgaccataac aggatgcacc
     3361 aggttatcac ggcgacgata atcgcaatca gcaccgcggc ggatcccata tcttttgcgc
     3421 gtccggaaag ctcatggtat tcagagccaa ttcggtcaac cactgcttcg atggcgctat
     3481 tgaggatttc cacaatcatc accagcatca cggagctgat aagcagcacg cgggtaatcg
     3541 cgtccacatc cagccagcag gcgatgacca ccgccaacaa taccgctacg ccttcctgac
     3601 ggaatgccgc ttcgttgatc catgcagcgc gtaaaccttt ccaggaatag ccagcagctt
     3661 tgataattcg ggtgaatcca gtggtattat tggccattga aagaaccttt ttacattatg
     3721 agcgtcaata tcagtgtacc gttaaatccg atgattcggg tttgcgtaac gccgcgcgaa
     3781 acatgagcgg ataccacaga atttcccatg actttctgct atccttgccg cgcatttgca
     3841 ttattaacca gaggctttac atcgtttatg tccggctggc cacgaattta ctacaaatta
     3901 ctgaatttac cattaagcat cctggtaaaa agcaagtcta ttccggcaga tcctgccccg
     3961 gaactggggc tggatacctc tcgtccaatt atgtacgttt taccgtacaa ctcgaaagca
     4021 gacttgctga cgttgcgcgc ccagtgtctg gcacatgacc tgcctgaccc gttagagccg
     4081 ctggaaatcg acggcacact actgccgcgc tatgtgttca ttcacggcgg gccgcgtgtg
     4141 ttcacctatt acacgccgaa agaagagtct attaagctgt tccacgacta tctcgatttg
     4201 caccgtagca acccaaatct ggatgtgcag atggtgccag tgtcggtgat gtttggtcgc
     4261 gcgccggggc gtgaaaaagg cgaagtgaac ccgccgctgc gtatgcttaa cggcgtacag
     4321 aaatttttcg ctgtactgtg gctcggtcgc gacagttttg tgcgtttctc accgtccgtt
     4381 tcgctgcgcc gtatggcgga tgaacacggc acggataaaa ctatcgcgca gaaactggcg
     4441 cgcgtggcgc gtatgcactt tgcccgtcaa cgtctggctg ccgtaggacc acgtctgcct
     4501 gctcgtcagg atctgtttaa taagctgctc gcctcccgcg ccattgccaa agcggtagaa
     4561 gatgaagcgc gcagcaaaaa aatctcccat gaaaaagcgc agcagaacgc gattgcgctg
     4621 atggaagaga ttgcggcgaa tttctcttac gagatgatcc gcctgactga ccgtattctg
     4681 ggctttacct ggaaccgcct ttaccagggc atcaacgtcc ataacgccga gcgcgttcgc
     4741 cagttggccc acgacggtca tgagctggta tatgtgcctt gccaccgcag tcacatggac
     4801 tacctgctgc tttcttacgt gctgtatcac caggggctgg tgccgccgca tatcgccgcc
     4861 gggatcaacc tgaatttctg gccggccggg ccgattttcc gccgtctggg ggcgttcttt
     4921 attcgccgta cgtttaaagg caataagctc tattccaccg ttttccgtga gtatctcggc
     4981 gaactgttca gccgtggtta ttccgtcgag tacttcgtgg aaggcggtcg ttcccgtacg
     5041 gggcgtttgc tggatccgaa aaccggtacg ctgtcgatga ccattcaggc gatgctgcgt
     5101 ggcggcacgc gtccgattac gctgattccg atctatatcg gttatgagca cgtcatggaa
     5161 gtgggtactt acgccaaaga actgcgcggt gcgacgaaag agaaagagag cctgccgcag
     5221 atgctgcgcg gtttaagcaa gctgcgtaat ctcggccagg gttacgtcaa tttcggtgaa
     5281 ccaatgccgt tgatgaccta ccttaaccag cacgtaccag actggcgtga atctatcgat
     5341 cccatcgaag cggtgcgtcc ggcatggtta acgccgacgg tcaataatat tgctgccgat
     5401 ctgatggtac gcattaacaa cgcaggcgcg gcaaacgcca tgaacctgtg ctgtactgcg
     5461 ctactggcat cacgtcagcg ctcactcacc cgcgagcagt taaccgagca actcaactgc
     5521 tacctggatc tgatgcgcaa cgtgccttac tccacggact ctaccgttcc ttcagccagc
     5581 gccagcgagc ttatcgatca cgcgctgcaa atgaacaagt ttgaagttga gaaagacact
     5641 atcggcgaca tcatcattct gccgcgcgag caagcggtgc tgatgaccta ctatcgcaac
     5701 aacattgcgc atatgttggt gctgccttcg ctgatggcgg caattgtcac ccagcatcgc
     5761 cacatctccc gcgacgtatt gatggagcac gtcaatgtgc tttacccaat gctgaaagcg
     5821 gagctgttcc tgcgctggga tcgcgacgag ttgccggacg ttattgatgc gctggcaaat
     5881 gagatgcaac gtcaggggct gattaccctg caagatgatg agttgcatat caacccggcg
     5941 cattctcgca cgctacagct gctggccgca ggcgcgcgcg aaacgctgca acgttatgcc
     6001 atcaccttct ggctgttgag cgccaacccg tcgatcaacc gcggtacgct ggagaaagag
     6061 agccgcaccg tcgcgcaacg tctctccgtg ctgcacggca tcaacgcgcc ggaattcttc
     6121 gacaaggcgg tgttcagttc tctggtgctg acgctgcgtg atgaagggta tatcagcgat
     6181 agcggcgatg ccgaaccggc agaaacgatg aaggtttatc agttgctggc ggagttgatt
     6241 acatcagacg tgcgtttgac gattgagagt gcgacgcagg gcgaagggta atcagagaga
     6301 attgccggat gcggcgcaaa cgccttatca ggcttaccat aatctgcaaa ttcaataaat
     6361 tgcgatctgt tgtgtaggcc tgagaagcgc aacgcatcag gcagcccaga agaaagccgg
     6421 atgatcatcc ggctttttta catcatcaga aatgccagta actcattgcc agccctaaaa
     6481 acagtaccag accaacatag ttattattca taaatgcttt aaagcaggct tcgcgctcgc
     6541 ggttggcaat cagtttttgt tgataaacaa acagcgcgcc agccaccaga attgaccagt
     6601 aatatcccca gcctaagcca tttaactcac cgataatcgc catcagcgcc agtacgccaa
     6661 tctgcaaaat tccaataatc aatttatcgt attggccgaa caggattgcg gtggatttaa
     6721 tgccaatctt cacatcatca tcacggtcaa ccatcgcata ctgcgtgtcg taagccaccg
     6781 cccagagaat attggcgagg aacattaacc agcaactcaa tggcaccgac tcactcacag
     6841 cagcaaaagc cattggaatc gaccagccaa acgccgcgcc cagcaccact tgcgggagat
     6901 gggtataccg cttcataaac gggtacaccc acgccagcgc tagcgcggca atcgacaaca
     6961 gaatggtcat cgtattcagc gtcagcacca gtaaaaacga aatcagtacc agcactacaa
     7021 acagcgcgcg cgcctctttc tctgttaccg cgccgctggg aagtggtcgg ttcgccgtgc
     7081 gcttaacatg accatcaaac ttgcggtcag cataatcatt caccacgcaa ccagcagcgc
     7141 gcatcagcca gacacccgcg acaaacaccg ccaggatcca gagctgggga acgcccggtg
     7201 tcgccaccca caacgcccat aatgttggcc agagcagcag taacgcgcca attggcttat
     7261 ccgtacgcat taagcgatga aacgccagca gcttattctg cgtcagactc cactccatat
     7321 tttttcctct tagtacaacg gtgacgccgg taaaaacagt tctgttagca acagcggttt
     7381 accgcttaat cgcagacggg aacgtcgccc ccacagtccg gcatcacggc ctatctcaat
     7441 aaagtcccgg gttaatgtcg atgatgtgaa cagatagcgt cctaacggcg ttttacccaa
     7501 tttttgtaac gccagctccg gcccgcttaa cgttgacaca ggaacgacgg tacgaccggc
     7561 aagccacggt tcaccatcgg cacataacaa aatttcacgt aaccagtaac gagactcttt
     7621 cggcagcagc ggcagttctt cggggatttc attctgctcg acaaaccctt cgcggatcat
     7681 cgtcacgctc accgtttttc cctgctgttc aaaacgtttt gtcatggaat cttccagcaa
     7741 cagccagtcg agcagttgcg gatccagggc agggatctct gtaaaatagc gcagcgcacg
     7801 cagttgcgtt aacgcggggt gtgacatgcc gaactctccg ttacataacg taaaggcatt
     7861 gtatcgcaga aaagtgttta cgggagatgt aaagcaaatt aagtgatcta atgcgcaaca
     7921 aaaacgcaac aatcgcatga agtggtaaaa cgctgttgaa ccttacgctt tgccgatgcc
     7981 cgtgctggcg agggcaacca taggggcatc accgcccagg atgccaggaa cggtgatgtt
     8041 ttcctttact taaatacctt tattctttat acgagtagct cgccaactcg cagtaaaaat
     8101 ggggaaaagt gcatgtttat cctgagattt ggagccgaaa agtgtttccg gagtaagatt
     8161 aacaggcaat atctcatgtc tggatttgta gatatacgtg gaattccaat tggtatcttt
     8221 tgtaggattc aacatatcat gtaagcgctg ggagcttacc accataactg gcccatcatc
     8281 gacagaggag aacaggat
//
LOCUS       DAZFFG010000170         8281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFFG010000170 DAZFFG010000000
VERSION     DAZFFG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.171"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            261..944
                     /locus_tag="ACHJ7J_004537"
     CDS             261..944
                     /locus_tag="ACHJ7J_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5002665.1"
                     /translation="MNLKKIFFSAVTVSVLCTLTGCDYIEEGKPESSLLKQQEEHNNK
                     IELIEKQQAQLKSQLETIQKQQTGIITSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSIPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            988..4205
                     /locus_tag="ACHJ7J_004538"
                     /pseudo
     CDS             988..4205
                     /locus_tag="ACHJ7J_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            4633..6288
                     /gene="lldP"
                     /locus_tag="ACHJ7J_004539"
     CDS             4633..6288
                     /gene="lldP"
                     /locus_tag="ACHJ7J_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5002666.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSVVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6288..7064
                     /gene="lldR"
                     /locus_tag="ACHJ7J_004540"
     CDS             6288..7064
                     /gene="lldR"
                     /locus_tag="ACHJ7J_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO5002667.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7061..8251
                     /gene="lldD"
                     /locus_tag="ACHJ7J_004541"
     CDS             7061..8251
                     /gene="lldD"
                     /locus_tag="ACHJ7J_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO5002668.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         2053 a         2009 c         2195 g         2024 t
ORIGIN      
        1 tccgtaaaaa tttttccaga tatggtttct tccatgtttt acatttatta ataatcatcc
       61 ttagaatcca caaaaataat atattcatac aaacacagac atcttttgaa acgaatgata
      121 catttttatg atgaatgctt aaaacaatat ttaatcgaca cccatcactg tcattaatgt
      181 aaagcaacat agatcaccaa taatacaatg gacaactcta ccggcattga tcgctggtcg
      241 gaggcaataa ggatttttac atgaacttga agaagatttt tttcagtgct gtcactgttt
      301 cagttctctg cacattaacg ggatgtgact acattgaaga aggcaagcca gaaagtagtt
      361 tactgaaaca acaagaagaa cataacaata aaattgagct aattgaaaaa caacaagcac
      421 agctaaagag tcaactggaa actattcaaa aacaacaaac tggcataata accagtacaa
      481 agactttaac tcacgtaatt aaatccgtta aagatcaaca aaacactttt atttttaccg
      541 agtttaatcc ggcaaaaacg aagtatttca tcctgaacaa tggttcggtg gctttagcgg
      601 gccgagtgtt atccattgac gcaacagaga atggcagtgt tattcatatt tcactggtca
      661 acttattaag tataccaatc tcaaacattg gttttaatgc gacatggggt ggtgaaaaac
      721 ctgtcgatgc caaagagttt gcccggtggc aacaattact tttcaacaca tcaatgaaat
      781 ccacattgaa attattacca ggtcaatggc aagacattaa tttgacactg aagggtgtat
      841 cgccgaataa cctgggatat ctgaagttag ccatcaatat ggagaatatt cagtttgaca
      901 accttccctc tgctgaaaat cggcagaaaa gaagcaaaaa ataaatgcag cttctgcgct
      961 tatatcaagg aatagagaac atcattaatg aacaaaatat ttaaagttat ctggaaccct
     1021 gcgacaggga attatactgt taccagcgaa acggcaaaaa gccgtggcaa gaaatccggg
     1081 cgcagtaagc tgttaatttc tgccgtggtt gcgggtgggt tgttgtcgtc gtttgaggca
     1141 ctggcaacag gtaatgaacc tggtacacct gttggcacag gatctggctg ggttgctata
     1201 ggccaggaat cacaggcaaa tgcttttacc gatggtaatg gtgccagtac tgcagtgggt
     1261 tataaagctt tcgctgaagg aacatggagt tccgctattg gcgccatgac ccatgcaatt
     1321 ggtccggctt caatggcatt cggggttaat gccgtctcaa ccggtcaacg aagtatcgct
     1381 atgggcgcat cgtcaagttc agtgggtcaa tattcgatgg cacttgggcg atatgcgaat
     1441 tcgggtggtg attattcgat cgcgcaaggg gataaatcca atgctgctgg tctgaatgcc
     1501 attgcagtgg gaagaagcag tagtgcggca ggcgagaaag ccactgcgct gggcaacgca
     1561 gctaaagcta ctgagattat gagtattgct cttggcgaca gcgccaatgc gtcaaaagcg
     1621 tattcaatgg cgctggtagc aagtagcgtc gcatctgaag aaaacgcaat tgccctgggg
     1681 cgtagcagtt agctagcggt actgacagcc tcgcattagg cagacaaaca cttgccagta
     1741 cagcgaacgc tattgcgata ggtgctgaga gcaaagccgc tgaaaatgcg acggccgttg
     1801 gtaccaaagc ggaggccaat ggacttaata gtatcgcgtt gggctctggg ggtattgcag
     1861 atgccgataa cacgattgct ctgggcaatc aaagcctggc agtagctgct ggtgcaattg
     1921 ccatcggtca ggggaataaa gctgacggcg caaatgctat cgcgctgggt aatggtagtg
     1981 ttacaggtgg cgcaaatgct attgctcttg gacaaggtag ttatgccggt ttagaaaatg
     2041 gcattgcaat tggtggtcaa gccagtgctc aagggacaaa ttccgttgct ctgggtgcgg
     2101 gttctgtagc gactgaagat aattccgtct ctgtgggtaa cacaacagat cagcgaaaaa
     2161 ttgtcaatat ggcagcaggt gatatcagca ctagcagtac tgatgccata aatggatcac
     2221 agctttatgc tatcagtaag tcagtagcgg accgtcttgg tgggggggct accgtcaatg
     2281 cgcaaggtgt cgttactgcc ccgaattaca gactgaaaaa tggtatttat ggcaatgttg
     2341 gcgatgcttt agcggatcta aacaataaca cgatacaatg ggactccgtt aaaaagggat
     2401 atagtgcggc acatggtaca gatgccacca gcaccatcac caacgttaaa gccggcacta
     2461 tttctgctac cagtacggat gcggttaacg gttctcagtt gaatgcgacg aatatgatga
     2521 ttgagcagaa cagccaaatc atcaatcagc ttgctggtaa caccgacgca acctatatcg
     2581 aagaaaatgg cgctggtatc aactacgttc gtactaacga caacggttta gcctttaacg
     2641 atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc gcgtctggtg
     2701 ccagcagcgt agccattggt cagaacagca atagcaccgt tgataccggt attgcgctgg
     2761 gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact agcgtaacgg
     2821 aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt gcattgtcaa
     2881 ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttcc gaagcccatg
     2941 acgccgttac ggttcgtcaa ttgcagaatg cgattggtgc ggtcgcaacc acgccaacca
     3001 agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa gactcgctgg
     3061 caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt ttgaacactc
     3121 tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc gcaaaccatg
     3181 caaacagtat tgcaatgggt aacggttctc agaccacccg tggtgcacag actggctaca
     3241 ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtt ggtagtgaag
     3301 acggtcaacg tcagatcacc aacgtcgcag ctggttcagc ggataccgat gcggtcaacg
     3361 taggtcagtt gaaagtaacg gatgcgcagg tttcccagaa tacccagagc attactaacc
     3421 tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac ggtattggcg
     3481 atatcgtcac caccggtagc accaagtact tcaagaccaa taccgatggt gtagatgcca
     3541 gcgcgcaggg taaagatagc gtcgcgattg gttctggttc cattgctgcc gcggataaca
     3601 gcgtcgcact gggtacaggg tctgtggcaa ccgaagaaaa tacgatctct gtaggctcat
     3661 ctactaatca acgtcgtatc accaacgtag ccgcaggtaa aaacgacacc gatgctgtta
     3721 acgtggcaca gttgaagtct tctgaggcgg gcggtgtacg ttacgacacc aacgctgatg
     3781 gttctgttga ctatagcaat atcaccctcg gtgggggcaa cggcggtacg actcgtatca
     3841 gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag ttgaagcaaa
     3901 gcgtgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat aacaaactgt
     3961 ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg accggtctgc
     4021 cgcaggctta cactccaggt gccagcatgg cctctattgg tggcggtact tacaacggtg
     4081 aatcggcagt tgctttaggt gtgtcgatgg tgagcgccaa tggtcgttgg gtctacaaat
     4141 tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc ggtattcagt
     4201 ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct gatgcgctac
     4261 gcttatcagg cctacagggt gattctgcac attcctatag gccgggtaag gtgttcacgc
     4321 cgcatctggc aagataaggc gcaccgggtc aacaatctaa gggcaattct ctgatgagga
     4381 ttgccctttt ctttaccaga catctccccc ccacaagaat tggccctacc aattcttcgc
     4441 ttatctgacc tctggttcac agttccccaa ttaaaactca cataaatgtt gccaatacat
     4501 aacatttagt taaccattca ttgtcattat ccctacacaa cataattggc agtgccactt
     4561 ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg cgtggactcc
     4621 aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa tatctggctt
     4681 tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat taagctcaaa
     4741 ctgaaaggat acgtcgccgc ctcatggacg gtggcaatcg cccttgccgt ggctttgctg
     4801 ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt cttctacggg
     4861 ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat ctcggtgaaa
     4921 accgggcagt ttgacatcat tcgctcgtct attctttcga taaccccaga ccagcgcctg
     4981 caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc aggctttggc
     5041 gcaccggtag caattaccgc cgcattgctg gtcggtctgg gctttaaacc actgtacgcc
     5101 gccgggctgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat gggcattcca
     5161 atcctggttg ccggacaggt aacaggtatc gacagctttg agattggtca gatggtgggg
     5221 cggcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat tatggacggc
     5281 tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg cgggcggctc gtttgccatc
     5341 gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat ctcttcgctg
     5401 gtatcactgc tctgcctgac gctgttcctc aaacgctggc agccagtgcg cgtattccgt
     5461 tttggtgatt tgggggcgtc acaggttgat atgacgctgg cccacaccgg ttacactgcg
     5521 ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt aacactgtgg
     5581 agtatcccgc cgtttaaagc cctgttcgca tcgggtggcg cgctgtatga gtgggtgatc
     5641 aatattccgg tgccgtacct cgataaactg gttgcccgta tgccgccagt agtcagcgag
     5701 gctacagcct atgccgccgt gtttaagttc gactggttct ctgccactgg caccgccatt
     5761 ctgtttgctg cgctgctctc ggttgtctgg ctgaagatga aaccgtctga cgctatcagc
     5821 accttcggca gcacgctgaa agaactggct ctgcccatct actccatcgg tatggtgctg
     5881 gcattcgcct ttatttcgaa ctattccgga ctgtcatcaa cactggcgct ggcactggcg
     5941 cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg ggtattcctg
     6001 accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa
     6061 caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg cgtcaccggt
     6121 aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa
     6181 gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg tatggtgggc
     6241 gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg attgttttac
     6301 ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc
     6361 tggaagcggg catgaagttg cccgctgaac gtcaactggc gatgcaactc ggcgtatcac
     6421 gtaattcact gcgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg ctcagtcgac
     6481 gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc
     6541 agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt ctggaagcac
     6601 gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca cctggcgaca
     6661 aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat atcgcctcac
     6721 aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc gtgctgctgc
     6781 aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga
     6841 tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct gtcattgacg
     6901 ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac cttagttttg
     6961 ttcacaccac aatgaaacga ttcgatgaag atcaggctcg ccacgcacgg attacccgcc
     7021 tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt ccgcagccag
     7081 cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact atatggatgg
     7141 gggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag aagtggcgct
     7201 gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc tgtttaatga
     7261 gaaattgtcg atgccggtgg cgctaggtcc ggtaggtttg tgtggcatgt atgcgcgacg
     7321 cggcgaagtt caggcagcca aagcggcgga tgcgcatggc attccgttta ctctctcgac
     7381 ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa tgtggttcca
     7441 gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag caaaagcagc
     7501 gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggagcgc gttatcgtga
     7561 tgcgcattct ggaatgagcg gcccgaacgc ggcaatgcgc cgctatttgc aggcggtgac
     7621 gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccgcatgatt taggaaatat
     7681 ttcggcttac ctcggcaaac cgaccggact ggaagattac atcggctggc tggggaataa
     7741 cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct gggatggccc
     7801 gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac gttttggtgc
     7861 tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac tctcttccgc
     7921 ccgtgcactg cctgctattg cagatgcggt gaaaggtgat atcgccattc tggcggatag
     7981 cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact
     8041 gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg tagctaacct
     8101 gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg cgaaatcgat
     8161 cagcgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc ctgcggcact
     8221 ggctcccatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc ctaaccctct
     8281 c
//
LOCUS       DAZFFG010000171         8218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFFG010000171 DAZFFG010000000
VERSION     DAZFFG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.172"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..2911
                     /gene="evgS"
                     /locus_tag="ACHJ7J_004542"
     CDS             <1..2911
                     /gene="evgS"
                     /locus_tag="ACHJ7J_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO5002669.1"
                     /translation="NVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVS
                     QNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGD
                     ILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITT
                     PYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKE
                     GELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALN
                     AIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVR
                     RRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLP
                     LENSESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEH
                     AVYICGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLEL
                     LSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQN
                     TCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKIT
                     TSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKEL
                     IKNMQGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLSILIADDHP
                     TNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGFELTRKLRE
                     QNSSLPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYR
                     HLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGA
                     ANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(2967..4112)
                     /gene="yfdE"
                     /locus_tag="ACHJ7J_004543"
     CDS             complement(2967..4112)
                     /gene="yfdE"
                     /locus_tag="ACHJ7J_004543"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO5002670.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4186..5130)
                     /gene="yfdV"
                     /locus_tag="ACHJ7J_004544"
     CDS             complement(4186..5130)
                     /gene="yfdV"
                     /locus_tag="ACHJ7J_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO5002671.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5200..6894)
                     /gene="oxc"
                     /locus_tag="ACHJ7J_004545"
     CDS             complement(5200..6894)
                     /gene="oxc"
                     /locus_tag="ACHJ7J_004545"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO5002672.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6948..8198)
                     /gene="frc"
                     /locus_tag="ACHJ7J_004546"
     CDS             complement(6948..8198)
                     /gene="frc"
                     /locus_tag="ACHJ7J_004546"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO5002673.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2399 a         1910 c         1719 g         2190 t
ORIGIN      
        1 aaatgtagtg aaatattata actcaccgcg tcaatataat tttttcttga ccagaaaaga
       61 atctgtcgtt cttaatgaag tactcaatcg atttgttgat gctttgacaa atgaagttcg
      121 ctatgaagta tcacaaaatt ggcttgatac aggaaacctg gcctttctga acaaaccatt
      181 agaactcact gaacatgaaa aacagtggat taagcagcat cccgatttaa aggtgctgga
      241 aaatccttac tcgcccccct attctatgac ggatgaaaat ggctcggttc ggggcgttat
      301 gggggacatt cttaatatta ttaccttgca aacaggttta aatttttctc cgatcaccgt
      361 ttcacacaat atccatgctg gaacacagct gaatcccggc ggatgggata tattacccgg
      421 tgctatttat agtgaagata gagaaaataa tgttttattt gctgaagcct tcataacaac
      481 gccttacgtt tttgtcatgc aaaaagcgcc tgacagtgaa caaacattaa aaaaaggaat
      541 gaaagttgcc attccatatt attatgagct tcattcgcaa ttaaaagaga tgtatccgga
      601 ggtagagtgg ataaaagtcg ataacgccag cgctgcattt cacaaggtca aggaaggcga
      661 acttgatgct ctggtcgcga cacagttaaa ttcacgttac atgatcgacc attactatcc
      721 taatgaactt tatcattttc ttattcccgg cgttccgaat gcatcgcttt cgttcgcttt
      781 tcctcgcgga gaaccggaac ttaaggatat tattaataaa gcactgaatg caatcccccc
      841 aagcgaagtt ctgcgcctga ccgaaaaatg gattaaaatg cccaatgtga ccattgacac
      901 atgggacctc tatagcgagc aattttatat tgttacgaca ttatccgttt tattagttgg
      961 cagtagcctt ttatggggat tctacctgtt acgctcagtt cgtcgtcgta aagtcattca
     1021 gggtgattta gaaaaccaaa tatcattccg gaaagcgctc tcggactcct taccgaatcc
     1081 aacttatgtt gtaaactggc aaggtaatgt cattagtcac aatagtgcat ttgaacatta
     1141 tttcactgct gattactaca aaaatgcaat gttgccatta gaaaacagtg aatcaccctt
     1201 taaagatgtt ttttctaata cgcatgaagt cacagcagag acgaaagaaa accgaacaat
     1261 atacacacag gtatttgaaa ttgataatgg catcgagaaa agatgcatta atcactggca
     1321 tacattatgt aatctgccag ccagcgaaca tgctgtttat atttgtggtt ggcaagatat
     1381 taccgagacg cgtgatttaa ttcatgcact cgaagtagag agaaataaag cgatcaatgc
     1441 aactgtcgca aaaagtcagt ttctggcaac aatgagtcac gaaataagaa caccaataag
     1501 ctccattatg ggcttcctgg aacttctgtc aggttctggt cttagcaagg agcaacgggt
     1561 ggaggcgatt tcactagcct acgccaccgg acaatcactc ctcggcttaa ttggtgaaat
     1621 ccttgatgtc gacaaaattg aatcgggtaa ctatcaactt caaccacaat gggtcgatat
     1681 ccctacttta gtccagaaca cttgtcactc tttcggtgcg attgctgcaa gcaaatcgat
     1741 cgcattaagt tgcagcagta catttcctga tcattacctg gttaagatcg accctcaggc
     1801 gtttaagcag gtcttatcaa atttactgag taatgctctc aaatttacca ccgagggggc
     1861 agtaaaaatt acgacctccc tggttcacat tgatgacaac cacgctgtaa tcaaaatgac
     1921 gattatggat tctggaagtg gattatcaca ggaagaacaa caacaactgt ttaaacgtta
     1981 tagccaaaca agtgcaggtc gtcagcaaac aggttctggt ttaggcttaa tgatctgcaa
     2041 agaattaatt aaaaacatgc agggtgattt gtcattagaa agtcatccag gcataggaac
     2101 aacatttacg atcacaatcc cggtagaaat tatccaacaa gtggcggctg tcgaggcaaa
     2161 agcagaacaa cccatcacac tacctgaaaa gttgagcata ttaatcgcgg atgatcatcc
     2221 gaccaacagg ctattactca aacgccagct aaatctatta ggatatgatg ttgatgaagc
     2281 cactgatggt gtgcaagcgc tacacaaagt cagtatgcaa cattatgatc tgcttattac
     2341 tgacgttaat atgccgaatg tggatggttt tgagttgact cgcaaactcc gtgagcaaaa
     2401 ttcgtcctta cccatctggg ggcttacagc caacgcacag gctaacgaac gtgaaaaagg
     2461 gttaaattgc ggcatgaact tatgtttgtt caaaccgttg accctggatg tactgaaaac
     2521 acatttaagt cagttacacc aggttgcgca tattgtacct cagtatcgcc accttgatat
     2581 cgaggccctg aaaaataata cggcgaacga tctacaactg atgcaggaga ttctcatgac
     2641 tttccagcat gaaacacata aagatttacc cgctgcgttt catgcactag aagctggcga
     2701 taatagaact ttccatcagt gtattcatcg catccacggt gcggctaaca tcctgaattt
     2761 gcaaaagttg attaatatta gccatcagtt agaaataaca cctgtttcag atgacagtaa
     2821 gcctgaaatt cttcagttgc taaactctgt aaaagaacac attgcagagc tggaccagga
     2881 aattgctgtt ttctgtcagc aaaatgacta aatagcggct cccacaatgt tcaaatgtgg
     2941 gagctattta ccagcacatc ttacatttat gatgagaact cctggcgaat ttgctcccca
     3001 tgctggtcga gcgttgccgc tcccggcata acatgcgggt ccgcgcagcc actgattttt
     3061 atcggattac ccggcatcat tattcccccg gcttcaatca acatatttct cgcctgagtt
     3121 tgtggcaaat taatggcctc agccacactt aataacggcg cgacgggcac accaacttca
     3181 tgtattctgg ctaaccaaac ttcagctgcc tgtgttttta acgtccgctc aatatattgt
     3241 ttaagaatag cctggttttg tacgcgtaaa atattgctgc taaatctggg atcattaacc
     3301 agttccgtaa gctccagtgc ctggcataac gcagagaaaa gcttgtcatt accacaacaa
     3361 atcgtaatcg gcttatcctg agtatcgaaa acatcaaaag gtgccatgta ggggtggcga
     3421 tttcccaaac gttgtggtga cttcccagtc gcgatatatg ccatcagccc gtgctccaga
     3481 aaactcagcg tggcatcaaa catcgctata tcgacatgcg cccctctctg gctcttttcg
     3541 cggccataaa gtgcactcac tattccactg aataaataga caccgccaca tagatccgca
     3601 agagaagtac caacgcgcac tggcggagca tcagggtatc ccgtttccat cattatcccg
     3661 ctcattgcct gaatgatggt atcgtaggca ggagcatctt ttagcggacc ggtatgtccg
     3721 aaacctgacg atgaagcata tatgaggcgc gggttgattt cttgtaacct ttcccatgaa
     3781 aaccccagtt tttccattgt acctgggcga aaattctcag ctaatacatc agcttgttta
     3841 agcatattta taaatatact tttatcgtga tcattcttta aatcaagaac cacactctct
     3901 ttaccatgat taataaaact gtaatagagt gactgtccat ccacataggg accaaatgtg
     3961 cgggtatcat caccatgacc tggtggctca actttaatta cccttgcgcc catattacaa
     4021 agaagttgag ttccgaaagg tccattaagg acatgtgtca tatcgataac taataagcct
     4081 tcaaacggcc ctttgctttc attatttgtc atttgcttat gccttgaaaa aagccgaagg
     4141 tgaaaagaca cttcggcatt tatgtaaaat caataacatg attaattatg aaaccagtcg
     4201 actgacataa atccacaagg gagccgtgac gacaaagccc agtacgctca cagccaatga
     4261 cgctgtacca gtgcgggtgt agacattaaa ccggctggca ataatgattc cggagaatgc
     4321 cggcggtaat gcgcctgcca gtaccatcat ctgcaaatgt tcgctgttca aatgacatgc
     4381 cataccaacg agaagcagcg ccagtggcat cagaatcagc ttcaggaagg tgttataagc
     4441 aatttcggca ctgaactcga atttatgtgc agccagagtc aaccccgcag cgaataccgc
     4501 tacccctgag ttagccttcg caatcagatt aaaggttggg tcccatgctg ccggaatttt
     4561 taccccaacc aacaccagga tcgttgccag aacaggtgcc catacaactg gctcctttgc
     4621 cgcagaaatt aatgcgctca gattactatt cttcttacca tccgctcctg aggaaggatt
     4681 cagcaaatac agaccaatag gaatagtaat tgcgttaaca ataatagaaa taattgctac
     4741 cactaaacct gttgatacgg aatcaccata aataggatcg agaactgcaa accccaggaa
     4801 tccaatggta ggtgaacctg caattaatgc acatacagct gcttctgcat gggtacgttt
     4861 aaaaaatttg tagcaaccaa accaggagaa aaagaaacat ccgacaataa ccacaagtga
     4921 tacaagggtc agacgagtgt ccgcaaaaat catttccctg tttgcccggg taatagatac
     4981 aaatagagcc gcaggaagag cgtagtttaa taccagttta ttaaatgccc gagcttgatc
     5041 ttctgaaaaa gtttctcgtc tgccgctaaa ataacccaac agcatgatta cgataatagg
     5101 caataaatcg ccaataaaaa atgttagcat aatagtattt ccgataaggg tcccctcacc
     5161 atcgcataat gagttggcaa gaggagacga tgtcagtatt taattaccag cgacttgttt
     5221 tgggttaagt ttggtaatat ggccactttc agttcctgct gcagggtcga tgaccacatt
     5281 aataatggtc ggtttgcgcg actgaatacc ggtggttaaa gcatgacgaa gttcatctgt
     5341 cgtggtgacg ttatagccaa cgccacgaaa cgcatccatt aatttgtcat accttgcatg
     5401 gtgcaacaga tctgttggtg atggtgcacc agcgccactg agatcaacac cgtctcctct
     5461 gtagatgccg ccattattaa aaataacgat cgtcaccggc aggttatatc gacaaatcgt
     5521 ttcaatctcc atcccactga aaccaaaagc actatcacct tcaatggcga caaccggaga
     5581 accagaggtc acgctagcac cgatggcata gcccataccg atgcccatga caccccaggt
     5641 accacaatcc agacgacgac gtggtttata catatcaata atatttcgtg cattatccag
     5701 ggtatttgca ccttcattaa ctaaataaat atcctggttc tcacgcaata catcgcgcac
     5761 agcacttaat gcattaaagt aatttaatgg ttgggtatct gtacttaatt tttcatgcat
     5821 tttttgtgca ttttgctgct tgtggatatt taaaatatcg cgccatacca gtggagtcgt
     5881 aaatgtgttt tgtttcagtt ctgccagcat accttgcata ctggatgcaa tatcgccaac
     5941 gactggcaca gcaatggggc ggttgctgtc aatttcctgc ggttcaatat ccagttgaat
     6001 aaactgtgta tctgccgccc atcctttttt accgtgtgcc agtaaccaat tcagtcgtgc
     6061 accaacaagc atgacaacgt cagcatttgc cagggcaaac gaacgcgcag ctgccgcaga
     6121 aagtggatgc gtatcttcga ggatcccttt cgccatagac attggcagga atggaatctg
     6181 ggcactttca ataaattcac gaagctgttc gtcagcttgt gaatacgccg cgcctttgcc
     6241 aaggataatt aatggccgtt cagcttttgc taaaagcgaa attgcgctag tgactgactt
     6301 cgggcatggc aataatgctg gcgacggatt ttcaactttt acaatcgtgg ttaacgcttc
     6361 gtctttttcc atcgtcgcgg ccaggacatt tgctggcaaa tcaagataaa ctccgccagg
     6421 gcgacccgat acggagaccc ggatagcgcg tgccaatgca atgccaagat cctgcggctg
     6481 attaacgcga aatgctgctt tggcatacgg ttttgccgca ttcatttggt ccagctcttc
     6541 ataatcacct tgctgtaggt caacgatcgc gcggtcgctg gagccgctaa tcatgatcat
     6601 cggaaaacca tttaccgttg cgttggccaa tgcggtcaaa ccattgagga atcctggcgc
     6661 agacactgtc aggcagatcc ccggtttttg ggtaagaaaa ccgcttgccg cagcggcata
     6721 gcctgccgac tgctcatgac gaaaaccaat ataacgaatg ccttccgcct gggcatggcg
     6781 cgccatatcc gtcacaggaa tacctacaac accataaata gtgtcaatat tattctgttt
     6841 taatgcttca acgatgatat gcataccatc tgtcatttga agttgatctg acatgattaa
     6901 taacctcgaa atttttataa attgggagcc agaagccccc gttaatatca gatggcgtgg
     6961 ttttgcttca ttgcagcaat ttcatcgtcg ctataaccca gctcctgcaa tacagcagcg
     7021 gtatgttcac ctaatagcgg cgcagcttta atatctggcg taaaggcaga gaatttcatt
     7081 ggacaaccaa ctgtcagata ttttccacgc aacggttgtt ccacttcgac aacactgcca
     7141 ctttggcgta aagagggatc aagtgaaatt tctttcatac ttaaaaccgg tgcacaagga
     7201 atatcaaatt gagtcaaata ggccaccgct tcatgtttat caatagtgac agtgtatttt
     7261 tcgatttcag caaaaatatc gaaaatatgt ggctgtcggg catgggctgt actgtatgcc
     7321 ggatcggtaa tccaatctgg tttgccgatg gctttacagg tgttttccca gttttgctcc
     7381 tgaatagtga aataaatata ggcgttagga tcggtttccc aacctttaca tttcaggatc
     7441 cagccaggct gaccgccacc acccgcatta cctccgcggg gaactgcatc accaaatgtg
     7501 ccattcggat actgcgggta ttcttccaga taacccaatt tatcgagacg ctgctggtcg
     7561 cgtaatttca cgcggcaaag gttcaatacg gcatcctgca ttgacatggt gactcgttgc
     7621 ccacgccccg ttttttcgcg atgcagcaaa gcagcaagta aaccgatcag taaatgcatt
     7681 ccggtgttgc tgtcacccaa cgctgcagcg cttaccagcg gcggaccgtc ccaaaaaccc
     7741 gtagtggatg ccgcgccacc cgctgcctga gcaacgtttt cataggcttt tacattcaca
     7801 taaggcgaac actcatcaaa ccctttgatc gaaccaaaaa tcagacgtgg attgatttct
     7861 tgaatatgct cccaggtgaa acccatgtga tcaatggccc ctggatgaaa gttctcgact
     7921 aagatatcag cttcgcggat cagcttttcc attacctctt tgccttccgc tgttttggta
     7981 tttaactcaa tagaacgttt gttactgtta agcatggtga agtaaagcgc atcgatatca
     8041 ggaatatctc gcagctggtg acgcgttacg tcaccaacgc cggggcgttc aattttaatg
     8101 acgtcagcgc caaaccaggc cagcatttga gtacaagatg ggccagattg cacaccggtg
     8161 aaatcgagaa ctttaattcc ttgaagtgga gttgacatag caatacctat tagttaat
//
LOCUS       DAZFFG010000172         8082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFFG010000172 DAZFFG010000000
VERSION     DAZFFG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.173"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            29..5449
                     /locus_tag="ACHJ7J_004547"
     CDS             29..5449
                     /locus_tag="ACHJ7J_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO5002674.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRVGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSVSGASLEVDNGQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSSEGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGKVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGEEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASSNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVNVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDNHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGSLTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSVSPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDLTKLGTGILT
                     LNNDSSAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMSGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPPDTPAPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSSAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5660..5926
                     /gene="hipB"
                     /locus_tag="ACHJ7J_004548"
     CDS             5660..5926
                     /gene="hipB"
                     /locus_tag="ACHJ7J_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO5002675.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5926..7248
                     /gene="hipA"
                     /locus_tag="ACHJ7J_004549"
     CDS             5926..7248
                     /gene="hipA"
                     /locus_tag="ACHJ7J_004549"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO5002676.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            7519..7788
                     /locus_tag="ACHJ7J_004550"
                     /pseudo
     CDS             7519..7788
                     /locus_tag="ACHJ7J_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2210 a         1717 c         2204 g         1951 t
ORIGIN      
        1 ctgtattgtt tgtatatgga gcgtttatat gaatagaatc tatcgcgtga tatggaattg
       61 cactctacag gtatttcagg cctgctcgga attaactcgc agggtaggta aaacatcaac
      121 ggttaatttg cgaaaatcct ctggactgac aacgaaattc agtagattga cgctgggtgt
      181 tttgctggca ctaagcggtt cagtgtctgg tgcgagtctg gaagttgata atggtcagat
      241 taccaatatt gatactgatg ttgcttatga tgcctacctg gttggctggt atggcactgg
      301 agtgcttaat attttggctg gcggtaatgc ctccttaacc actattacta ccagcgtcat
      361 tggcgctaat gaggactcag agggcaccgt taatgttttg ggtggcacct ggcgattgta
      421 tgatagtgga aataatgcaa ggcctttaaa tgtgggtcaa tccggaacgg ggacgctgaa
      481 tattaaacag aagggtcacg tcgatggagg ctatttaagg ctaggttctt cgacaggagg
      541 cgtcgggacg gtcaatgttg agggagagga ctctgttctg acgaccgaat tattcgaaat
      601 agggagttac ggtacgggct cattaaatat tacggataaa ggttacgtca cgagttcaat
      661 agtcgccatt ttaggatatc aagcgaacag taatggtaag gttgtcgttg aaaagggtgg
      721 cgagtggcta ataaaaaata atgattcctc aattgaattt caaattggta atcaaggaac
      781 tggggaggcg actattcgcg agggtgggct gattacggct gaaaatacga ttatcggtgg
      841 caatgccacc ggtatcggaa ccctgaatgt gcaggatcaa gactctgtca tcacggtacg
      901 cagactctat aatggatatt tcggtaatgg cacagtcaat atttccaata atggactgat
      961 taataacaaa gaatattcat tggtgggcgt tcaggacggt tcccacggtg tcgtcaacgt
     1021 gaccgataaa gggcattgga atttcctcgg aacgggcgaa gctttccgct atatctatat
     1081 cggtgatgct ggcgacggtg aacttaatgt ctcgagtgaa ggcaaagtag attcgggaat
     1141 tatcactgcg gggatgaaag aaacaggcac aggcaacatt actgttaagg ataagaactc
     1201 cgttatcact aatctcggaa ctaatcttgg ttatgacggc cacggcgaaa tgaatatcag
     1261 taatgagggg cttgttgtca gcaacggagg aagctcactc ggttatggag aaaccggcgt
     1321 cgggaaggtt agcatcacca cgggggggat gtgggaggtc aataagaatg tctataccac
     1381 cattggtgtt gcgggcgtcg gaaacctcaa tattagcgat ggcggtaagt tcgtatcgca
     1441 aaatattact tttttgggcg ataaagcaag cggtatcggc acactgaacc tgatggatgc
     1501 gacatcgtcg ttcgatactg tgggtatcaa tgtcggtaat tttggtagcg gtatcgtaaa
     1561 tgtcagtaat ggtgccaccc ttaattcaac gggctatgga tttatcggag gaaatgcctc
     1621 cggtaagggg atagttaata tttcaacgga tagtctctgg aatttaaaga cgtcttctac
     1681 taacgcccaa ttgctacagg tcggtgtatt aggcacgggt gaactgaata ttaccaccgg
     1741 aggtatagtt aaagcgcgtg atacacagat agctctcaat gacaaaagta agggcgacgt
     1801 gagggtggat gggcagaact ctcttcttga aacattcaat atgtacgtag ggacatctgg
     1861 tacgggtacg ttaaccctga cgaatagcgg cacgctgaat gtcgaaggtg aagaagttta
     1921 cttaggtgtt tttgaacctg ctgtaggaac gctaaacatt ggtgcagctc acggtgaggc
     1981 ggcggcagat gccgggtata taaccaacgc gacgaaagta gagtttggtt ctggcgaagg
     2041 tgtttttgtc tttaatcata caaataacag cgatgccggc taccaggtcg atatgctgat
     2101 tacaggtgac gataaagacg gaaaagtgat ccatgatgca ggccatacgg tgttcaatgc
     2161 agggaatact tatagcggta aaacgctggt caatgacggc ctcctgacca ttgcgtctca
     2221 tacggcagat ggggtaacgg gcatgggttc gagtgaagta accattgcaa gccccggtac
     2281 gctcgacatt ctcgcatcat cgaacagtgc aggagattac acgctgacca atgcgctcaa
     2341 aggcgatggc ttgatgcgag ttcagctgtc atcctccgac aagatgtttg gctttacaca
     2401 tgcaacaggg actgaattcg ccggtgttgc ccaactgaaa gacagtacct tcactctgga
     2461 acgcgacaac accgctgcgc ttactcacgc gatgttgcag tctgacagtg aaaataccac
     2521 atcggtaaac gtgggagagc aatccattgg tggactggcc atgaatggcg gtacgctcat
     2581 tttcgatacg gatattcctg ctgcgacgct tgcagaggga tatatcagcg tcgatacgct
     2641 ggttgtcggc gcgggtgact acacctggaa aggacgtaac tatcaggtaa acgggacggg
     2701 cgacgtgctt atcgacgtgc ctaaaccgtg gaatgatccc atggcgaata accctctgac
     2761 gacgctcaat ttgctggaac acgatgataa ccatgtcggc gttcaactgg tgaaggcgca
     2821 aacggttatt gggtcgggtg gctcattaac gttacgtgat ttacagggcg acgaggtgga
     2881 agcggacaaa acgttacaca ttgcgcaaaa cggaacggtg gtcgccgagg gtgattatgg
     2941 attccgcctc acgaccgcac caggtgatgg tttgtacgtt aactatgggc tgaaagcgct
     3001 gaacatccat ggagggcaaa agctgacgtt agccgaacat ggcggagcct atggcgcaac
     3061 ggccgatatg tcggcaaaaa tcggtggtga aggggatctg gcaatcaata cggtgcgaca
     3121 ggtttcgctt tccaacggtc agaacgacta tcagggggca acctacgttc agatggggac
     3181 attacgtacc gatgcggatg gcgcgctggg caacacccgg gaactgaaca tcagcaacgc
     3241 agccatcgtc gatcttaatg gatcgacgca gacggtagag acattcaccg ggcagatggg
     3301 ttcgactgtt ttgttcaaag aagggtcgct gacggtaaat aaaggtggga tcagtcaggg
     3361 tgaactgaca ggtggcggaa acctgaatgt tacaggggga acgctggcta tcgaggggct
     3421 taatgcacgc tacaatgcgt taaccagcgt tagcccaaat gcggaagtca gcctcgataa
     3481 tacgcagggg ttaggcagag gaaatattgc caatgacggt ctgttaacgc taaaaaacgt
     3541 gactggcgaa ctgcgtaata gcataagcgg gaagggtatc gtgagcgcaa ccgccaggac
     3601 agatgtagag ctggatggcg ataatagccg ctttgtgggg caattcaaca ttgatacagg
     3661 cagcgcgctc agcgtcaacg agcagaaaaa cctgggtgat gcttccgtta tcaataatgg
     3721 cctgctcacc atctccactg agcgtagctg ggcgatgacg cacagtatca gcggtagcgg
     3781 tgatttgaca aaactgggta ccgggatcct gactcttaac aacgattcct cggcgtatca
     3841 gggtacgacg gatatcgtgg gtggtgaaat tgctttcggt tccgactctg ccattaatat
     3901 ggcaagtcag cacattaata tccataacag cggtgtgatg tcgggaaatg tcaccactgc
     3961 aggtgatgtg aacgttatgt ctggggggac actgcgtgtc gccaaaacca caatcggcgg
     4021 caacctggag aatggcggca cggttcaaat gaacagtgaa ggaggaaaac cggggaatgt
     4081 actgaccgtt aacggtaact ataccggaaa caatggcctg atgacgttca acgcgacgct
     4141 gggcggcgat aattcgccca ccgataagat gaacgtgaaa ggcgataccc aagggaatac
     4201 tcgcgttcgg gttgataaca ttggcggcgt cggtgcacaa acggtcaacg gtattgaact
     4261 cattgaggtt ggcggtaatt ctgcaggtaa cttcgcgctg accaccggaa ctgtcgaagc
     4321 tggggcatac gtctacacgc tggctaaagg gaaggggaat gacgagaaaa actggtatct
     4381 gaccagtaaa tgggacggcg taacgccacc ggatacaccc gctcccatta ataatccccc
     4441 tgttgtggat ccggaaggcc catcagttta tcgcccggag gccggaagct atatcagcaa
     4501 cattgccgca gccaactcgc tgtttagcca tcgcttacac gaccgtctgg gtgagccgca
     4561 atatacagat tcactgcatt ctcagggttc agcaagcagt atgtggatgc gtcatgtcgg
     4621 ggggcacgaa cgttccagtg ccggagacgg ccagctaaat actcaggcta accgctatgt
     4681 attgcagcta ggaggcgatt tggcgcagtg gagtagcaac gcgcaggatc gctggcatct
     4741 tggcgtgatg gcaggctacg ccaatcagca cagtaatact cagagtaatc gtgtgggtta
     4801 taaatcggat gggcgcatca gcggttacag cgctgggctg tacgcgacct ggtatcagaa
     4861 cgatgcgaat aagaccggcg cttatgttga cagctgggcg ctgtataact ggtttgataa
     4921 cagcgtcagt tccgataacc gttctgctga cgactatgat tctcgcggtg tgacggcctc
     4981 tgttgagggt gggtatacct ttgaagcggg aacatttagc ggcagcgaag ggacgctgaa
     5041 tacctggtac gtccagccac aggcgcaaat cacctggatg ggtgtgaaag attctgacca
     5101 tacccggaaa gacggaacac ggattgaaac ggaaggtgac ggaaacgtgc aaacgcgact
     5161 tggggtgaaa acctacctga atagccatca ccagcgtgac gatggtaaac agcgtgagtt
     5221 ccagccttac attgaagcga actggatcaa caatagcaaa gtctacgccg tgaagatgaa
     5281 tggtcaaacc gtaagccgtg atggtgcgcg taatctcggt gaagtacgta ccggggttga
     5341 ggcgaaagta aataacaacc ttagcctgtg ggggaatgtc ggtgtgcaac taggtgataa
     5401 aggctatagc gatactcagg gcatgctggg agtgaaatat agctggtaaa ccgtataagc
     5461 cgcatgtcga gatggcatgc ggcttaatat tgccaacttc aaaaggcttt taaagcctcc
     5521 cttttatccg cgatcgcgga tatctcagcg tttatcccgt acagcggata agatgtgttt
     5581 ccagattgac tttatcctca ctaaaggata aaactgataa tatcccctta agcggataaa
     5641 tttgctgtgg acgtatgaca tgatgagctt tcagaagatc tatagcccaa cgcaattagc
     5701 gaatgcaatg aaactggttc gccagcaaaa tggctggacg cagagcgagc tggcgaaaaa
     5761 aatcggcatt aagcaggcga cgatttccaa tttcgaaaac aaccctgaca atacctcgct
     5821 cacgacattt tttaagattt tacagtcgct tgaactctca atgacgctat gcgacacgaa
     5881 aaatgcctcg ccagaatcaa cagaacagca agatctggag tggtaatgcc caaactagtc
     5941 acttggatga acaaccagcg ggtaggcgag ttaacgaagt tagccaacgg cgcgcacacc
     6001 tttaagtatg caccggagtg gttagccagc cgttatgcca gaccgttgtc actttcgctg
     6061 ccattgcaga gggggaatat cacctctgat gccgtattta acttcttcga taacttgtta
     6121 cccgatagcc cgattgtacg tgaccggatc gttaaacgtt atcatgccaa atccagacaa
     6181 ccgtttgatt tattgtcaga aatagggcga gacagcgttg gtgccgtgac gttaataccc
     6241 gaagacgaaa ccgtaacgcg tccgataatg gcatgggaaa agcttactga agccagactt
     6301 gaagaagtat taacggctta taaagcagat atcccgctag gcatgattag agaagaaaat
     6361 gactttcgca tctcggttgc tggcgcacag gagaagacag cactgctcag aataggcaat
     6421 gactggtgca ttccgaaagg aataacgccg acgacgcaca tcattaaatt accgattggg
     6481 gaaatcaggc agcccaatgc gacgctcgat ctcagccaaa gcgttgataa tgagtattac
     6541 tgtctgctgc tggcgaaaga acttgggttg aatgttccgg acgcagaaat cattaaagcg
     6601 ggaaatgtgc gcgcgttagc ggtcgaacgt tttgacaggc gttggaatgc tgagcgaacg
     6661 gttttacttc gcttgccaca ggaggatatg tgtcagacat tcggtttacc ttcatcggtg
     6721 aaatatgaat cagatggagg cccaggcatc gcgcggatca tggctttttt gatggggtcc
     6781 agcgaggcgc tgaaagatcg ctatgatttt atgaaattcc aggtctttca gtggttgatt
     6841 ggcgcaacgg atggccatgc aaaaaacttc tccgtattta ttcaggctgg cggcagttat
     6901 cgactcacgc cattttacga catcatttca gcatttccgg tattgggtgg cgcgggaata
     6961 cacatcagcg atctcaaact ggcaatgggg cttaacgcat ccaaaggcaa aaaaacggca
     7021 atcgataaaa tttatccgcg acattttttg gcgacagcaa aggtgctgag attcccggaa
     7081 gtgcagatgc atgaaatcct gagtgacttt gccagaatga ttccggcagc actggataac
     7141 gtgaagactt cattaccgac agattttcca gagaacgtgg tgacggcagt tgaaaccaat
     7201 gtgttgaggt tgcacggtcg gttaagccga gaatacggta ttaagtaaga gtaagatatg
     7261 gggtttggtc gttgttaatg agcaggacaa tcatgaccgc caacgactag attcttcctt
     7321 ggtttatgtt ggtgcgcata atgtatatta tgttaaatcg tatctgagtg atacgaagtg
     7381 attttccggt agtcgttatt cgttcaggat tccagaactt aagcctctcc tctttcataa
     7441 ttggcctgtg catgccggat ataggctggc tggtatgcag atttgccgtt tgccgcatct
     7501 tcccagcgtt gaagactcaa gctggcaaac cgcgtgcaaa tgagcacacc ctctttccat
     7561 caacacttca ggcagcttgc gggaatgagt ccactccgtt atcagaaatg tctacgtctc
     7621 aatgaagtca ggcgaacgat gctgaatgaa cattacgatg tcaccccagc agcctatgct
     7681 gtcggttacg aaagcttatc ccatttcagc cgggaatatt tgcggatgtt tggagagtca
     7741 cctaagagag atattaccag gttaagaaag tctgtcggtc agctttaata aaccctgcac
     7801 ttatctgttt agagcaattc ggtgttagtt acatcaggca aacattaact atagctaatg
     7861 atttatagcc atataaacca ttagtagccc ttgacctttc ctgacacttc ccttgttcgc
     7921 aataatattc tgtttagtca cggtttataa ataacatgca taatgaaatc gtcatgttat
     7981 ttagcgacat aaaatgtttt tgcaaatact ctcttcaaca agtgcagcaa taaacttgtt
     8041 gtgattaggt gtccttgtga tttaaagata tcatcgttct tc
//
LOCUS       DAZFFG010000173         8044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFFG010000173 DAZFFG010000000
VERSION     DAZFFG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.174"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(39..1289)
                     /gene="icd"
                     /locus_tag="ACHJ7J_004551"
     CDS             complement(39..1289)
                     /gene="icd"
                     /locus_tag="ACHJ7J_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO5002677.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1462..2115
                     /gene="rluE"
                     /locus_tag="ACHJ7J_004552"
     CDS             1462..2115
                     /gene="rluE"
                     /locus_tag="ACHJ7J_004552"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO5002678.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2125..2586
                     /gene="nudJ"
                     /locus_tag="ACHJ7J_004553"
     CDS             2125..2586
                     /gene="nudJ"
                     /locus_tag="ACHJ7J_004553"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO5002679.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2640..3746
                     /gene="mnmA"
                     /locus_tag="ACHJ7J_004554"
                     /gene_synonym="trmU"
     CDS             2640..3746
                     /gene="mnmA"
                     /locus_tag="ACHJ7J_004554"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO5002680.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3782..4423
                     /gene="hflD"
                     /locus_tag="ACHJ7J_004555"
     CDS             3782..4423
                     /gene="hflD"
                     /locus_tag="ACHJ7J_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO5002681.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4427..5797
                     /gene="purB"
                     /locus_tag="ACHJ7J_004556"
     CDS             4427..5797
                     /gene="purB"
                     /locus_tag="ACHJ7J_004556"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO5002682.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5965..6636
                     /gene="phoP"
                     /locus_tag="ACHJ7J_004557"
     CDS             5965..6636
                     /gene="phoP"
                     /locus_tag="ACHJ7J_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO5002683.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6636..>8044
                     /gene="phoQ"
                     /locus_tag="ACHJ7J_004558"
     CDS             6636..>8044
                     /gene="phoQ"
                     /locus_tag="ACHJ7J_004558"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO5002684.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGA"
BASE COUNT         1982 a         2049 c         2053 g         1960 t
ORIGIN      
        1 tgttatacgc ccgtttaaat atttaacaca tgtagtgatt acatattctt gatgatcgca
       61 tcaccaaact ctgaacattt cagcagttta gcgccttcca tcaaacgttc gaagtcatag
      121 gttacggtct tcgcattgat tgcgccttcc atacctttaa caatcaggtc tgcggcttcg
      181 gtccaaccca tgtggcgtaa catcatctca gcagagagaa taatagagcc tgggttaact
      241 ttgtcctgac cggcatattt cggcgcagta ccgtgggtag cttcaaacag ggcgcattcg
      301 tcaccgatgt ttgcgccagg ggcgataccg ataccgccaa cctgcgctgc cagggcgtca
      361 gaaatgtagt caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc
      421 aggatctgtt gcaggaatgc atcagcaatc acgtctttaa tgacgatctc tttgccggta
      481 ttcgggtttt taactttcag ccacgggccg ccgtcgatca gttcaccgcc aaattcttca
      541 cgcgccagct ggtagcccca gtctttaaac gcgccttcgg tgaacttcat gatattgcct
      601 ttgtgtacca gagtcacaga gtcacgatcg ttagcaattg cgtattcgat cgctgcacga
      661 accagacgtt tggtgccttc ttccgaacac ggcttaatac cgataccgca atgttccggg
      721 aagcgaattt tcttcacgcc catctcttca cgcaggaatt taatcacttt ctcagcgtcg
      781 gcagagtcag ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata
      841 accatatcgg tcagttcagg gtgtttaacc gggcttggag tgccctgata gtaacgtacc
      901 ggacgcaggc agatgtagag atccagttcc tggcgcaggg caacgttcag agagcgaata
      961 ccgccaccaa ccggagtggt cagcggacct ttaatggcaa cgcgatattc acgaatcaga
     1021 tcaagggttt cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg
     1081 gtgtaaattt ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg
     1141 tcgaccactt tcagcatggc tggggttacg tctacaccga ttccatcacc ttcaatgtaa
     1201 gggataatcg gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct
     1261 tgtgccggaa caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct
     1321 cgtctgccac cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt
     1381 tttttgttaa tgatttgtaa ttggcttgtc aatactaccc tattgtttgc cgtcatgaaa
     1441 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     1501 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac caggcgtaaa
     1561 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     1621 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     1681 gcagcaggtc gtcttgaccg cgatagcgaa gggttattgg tgctgaccaa taatggtgca
     1741 ctacaggcgc gtttaaccca gccaggcaaa cgcaccggaa aaatctatta tgtgcaggtg
     1801 gaaggcattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     1861 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac ctgcgtggtt atggccgcgg
     1921 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     1981 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     2041 ctgattcgct atgcgatggg tgattactct ttggataatc ttgctaatgg cgaatggcga
     2101 gaagtgacag attaaggatg caaaatgttt aaaccgcacg ttaccgttgc ttgcgtggtg
     2161 cacgcagaag gcaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     2221 caaccggcgg ggcatctgga agcggatgaa accttagtgg aagccgccgc ccgtgagctg
     2281 tgggaagaaa ccggcatcag cgcgcagccg caacacttta ttcgtatgca tcagtggatt
     2341 gcgccagata aaacgccgtt tttgcgtttc ctttttgcca ttgagcttga gcaaatatgc
     2401 ccgacccagc ctcatgacag cgatatcgac tgctgccgtt gggttagcgc cgaagaaatt
     2461 ttacaggcgt caaatcttcg ttcgccactg gtggcggaaa gtattcgttg ttatcaaagc
     2521 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     2581 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     2641 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgttgat tcctccgttt
     2701 ctgcctggct attgcaacaa cagggatatc aggtcgaagg cctgtttatg aagaactggg
     2761 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     2821 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     2881 acgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     2941 tctgcaacaa agagatcaaa tttaaagcct tcctcgaatt tgccgccgaa gatttaggtg
     3001 ccgattatat cgctaccggt cattacgttc gtcgggccga tgtcgatggc aagagccgcc
     3061 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     3121 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     3181 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     3241 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggcgcaa ccgggcaaaa
     3301 tcattaccgt cgatggcgat gaaattggtg agcatcaggg gctgatgtat cacaccctcg
     3361 gtcagcgcaa aggcctgggt atcggtggca ccaaagaagg caccgaagaa ccgtggtatg
     3421 tggtcgataa agacgtcgaa aacaacattc tgattgttgc gcagggccat gaacacccgc
     3481 gtctgatgtc tgtcggtctg attgcccagc agctgcactg ggtcgatcgt gaaccattca
     3541 ccggtactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccctgcaccg
     3601 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgagcccgtt gccgccgtga
     3661 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     3721 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     3781 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     3841 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     3901 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     3961 caacctgcgc gtcgggctgg aaaccttgct cggcgtgctc aatgccagca gtcgccaggg
     4021 cttaaacgcc gaattaaccc gctacacact cagcctgatg gtgcttgagc gcaaactctc
     4081 ctcagcgaaa ggtgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     4141 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     4201 tgtgattagc ccgcttggcc cgcgcattca ggtcaccggt tcccctgctg tactgcaaag
     4261 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     4321 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     4381 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     4441 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     4501 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     4561 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     4621 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     4681 cgagcgtact actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     4741 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     4801 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     4861 gtactggcgt caactaattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     4921 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     4981 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     5041 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     5101 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     5161 tacccagatt gaaccgcacg actacattgc cgaactgttt gattgcgttg cgcgcttcaa
     5221 caccattctg atcgactttg accgtgacgt ctggggttat atcgccctta accacttcaa
     5281 acagaaaacc atcgccggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     5341 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     5401 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgttgcgtaa
     5461 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     5521 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     5581 ggcagaacca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaaatt
     5641 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     5701 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     5761 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     5821 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     5881 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     5941 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     6001 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     6061 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     6121 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
     6181 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
     6241 gccggtgctg atgattacgt gactaaaccg tttcatattg aagaggtgat ggcgcgaatg
     6301 caggcattaa tgcggcgtaa tagcggcctg gcttcacagg tcatttcgct cccaccgttt
     6361 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
     6421 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
     6481 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
     6541 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cccaagaagt gattaccacc
     6601 gttcgcggcc agggctatct gttcgaattg cgctgatgaa aaaattactg cgtctttttt
     6661 tcccgctctc gctgcgggta cgttttctgt tggcaacggc agcggtagta ctggtgcttt
     6721 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
     6781 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
     6841 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
     6901 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
     6961 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
     7021 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
     7081 atgatgacga cgcggagatg acccactcgg tggcggtaaa cgtctacccg gcaacatcgc
     7141 ggatgccaaa gttaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
     7201 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
     7261 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
     7321 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
     7381 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatatc
     7441 gtacaacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
     7501 cgctgcgttc tctgcgtagt gagaaaatga gcgtcagtga tgctgagccg gtaatgctgg
     7561 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
     7621 gcgggacatt gcttagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
     7681 cggcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
     7741 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaat gtgctggata
     7801 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
     7861 tctatattgt ggtcgaggat gatggacccg gtattccatt aagcaagcga gaggtcattt
     7921 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
     7981 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgcttggcg
     8041 gtgc
//
LOCUS       DAZFFG010000174         7949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFFG010000174 DAZFFG010000000
VERSION     DAZFFG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.175"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            1..126
                     /locus_tag="ACHJ7J_004559"
                     /pseudo
     CDS             1..126
                     /locus_tag="ACHJ7J_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            121..866
                     /locus_tag="ACHJ7J_004560"
                     /pseudo
     CDS             121..866
                     /locus_tag="ACHJ7J_004560"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
     gene            1229..2266
                     /gene="dusA"
                     /locus_tag="ACHJ7J_004561"
     CDS             1229..2266
                     /gene="dusA"
                     /locus_tag="ACHJ7J_004561"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO5002685.1"
                     /translation="MYGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCNFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSYTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2400..2642
                     /gene="pspG"
                     /locus_tag="ACHJ7J_004562"
     CDS             2400..2642
                     /gene="pspG"
                     /locus_tag="ACHJ7J_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO5002686.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVAMAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2808..3791)
                     /gene="qorA"
                     /locus_tag="ACHJ7J_004563"
     CDS             complement(2808..3791)
                     /gene="qorA"
                     /locus_tag="ACHJ7J_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO5002687.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3874..5289
                     /gene="dnaB"
                     /locus_tag="ACHJ7J_004564"
     CDS             3874..5289
                     /gene="dnaB"
                     /locus_tag="ACHJ7J_004564"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5002688.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5342..6421
                     /gene="alr"
                     /locus_tag="ACHJ7J_004565"
     CDS             5342..6421
                     /gene="alr"
                     /locus_tag="ACHJ7J_004565"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO5002689.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPRCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGDGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6674..7867
                     /gene="tyrB"
                     /locus_tag="ACHJ7J_004566"
     CDS             6674..7867
                     /gene="tyrB"
                     /locus_tag="ACHJ7J_004566"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO5002690.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPAHSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1952 a         1871 c         2080 g         2046 t
ORIGIN      
        1 gatatatatg gtaagtctat tgattattca gcgctgcgtc atcgggatat aattattgca
       61 aaaatagatt tgtttattca aagaatcact cataatttat ggcatgcacg taaaaaaaat
      121 gtgtttttaa ttgagcaaat aactaatctc aaagagtggg ttaataagta tattggagat
      181 tgcactgatg aggaattaaa cgatcgtgac tttattgcat cagttgttga ccgggctatt
      241 tttcatttcg cggttaatag tatatgtaat actgagggta ataaagatgc aacgcccata
      301 gaacgatata cttttgatgt agaaaccaag aatggccttc cctctacggt tcagctattt
      361 tatgaggaat ctaaagataa tgaaccttta gcgaatatac atttgcaagc aataggttct
      421 ggttttttaa cgtttgttaa tgcatgtcag aaatatgatg acaacagctt aaaattattc
      481 gcttcgctgt tgatttcact ttcatactct agtgcctaca cagatttagc aggagcagaa
      541 aaagtgaata ttaatgataa taatgggaac tatctgacag ctcagtttga agagttatct
      601 aacgtgatat gaagaagtac ctgggagaga tgaagcgtct ggcagatggg ggagaaatga
      661 attttgatgg ctatctggat aagatgtcac atctggtgaa tgaaggaaag ctcgcccctg
      721 atattttaag caaaatgcga gatgcagcac cgaaattaat tgactttgct aagtcgtttg
      781 acccaaactc aaaggaaaag attaaaatat atacagacac ttctaaatta atttatgatt
      841 tattcggggt taaatcggag aaataatatg tgaagttctt cgatggtatg gaaggcatta
      901 cataaaagaa cccaatgctt attgggttct ttttttctct atcagtgcta ttagcaggga
      961 gagatatcac cggagtttaa tgtgtgattt ttatttatcg tcgaacctgg attgtttatc
     1021 attggcctta acaaagctaa cggctaataa gattatttcc atcacttcgt gagagcttca
     1081 tgccttgaga ggatctcaat tttcttttgc aatgagacag gcgcttcctg ttgttatggt
     1141 atagtatccc gctattgagc ctcctgaaca gtgatgctga ataacataac cccatgatat
     1201 atcgataaaa taatttctac atttgaaaat gtacggtaat tctgaaatgc aaaaaatcaa
     1261 ccaaaccagc gcaatgcctg aaaaaactga cgttcactgg agtggtcggt ttagcgttgc
     1321 accaatgctc gactggacgg acagacattg ccgctatttc ttgcgtctgc tttcccgcaa
     1381 tacgttgctg tataccgaaa tggtgactac aggggcgatt attcacggta aaggtgatta
     1441 tctggcgtac agtgaagaag aacatccggt agcgttgcaa ctcggcggta gcgatccggc
     1501 ggcgctggcg cagtgtgcga agctggcaga agcgcgtgga tatgatgaga tcaacctgaa
     1561 tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt ggtgcgtgtc tgatgggtaa
     1621 tgcgcagctg gttgccgact gcgtgaaagc gatgcgcgat gtggtgtcga ttccggtgac
     1681 ggtgaaaacg cgtattggca tcgatgacca ggacagctat gaatttctct gcaatttcat
     1741 caacaccgtt tccggcaaag gcgagtgtga gatgttcatc atccacgcac gtaaagcctg
     1801 gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg ccgctcgatt atccgcgtgt
     1861 gtatcaactg aagcgtgact ttccgcatct gacgatgtcg attaacggtg gtatcaagtc
     1921 gctggaagag gctaaagcgc atttgcaaca tatggatggc gtaatggtcg ggcgcgaagc
     1981 gtatcagaat ccgggtattc tggcggcggt agaccgagaa atctttggtt cctcgtatac
     2041 cgatgccgat ccggtggcgg tagtgcgtgc catgtatccg tacattgagc gtgaactcag
     2101 ccaggggacg tatctcgggc atattacccg gcatatgctg ggtctgttcc agggtattcc
     2161 tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc cataaagcgg gtgcagacat
     2221 taatgtgctg gaacacgcgc tcaaactggt ggcggataag cgttaacttt tcaccaaaaa
     2281 atagtcaaat tcaccacgcc ctgcgcaccg tcgcggggcg ttttgctgtt aaatcaatag
     2341 attatttttg gcatgattct tgtaatgcca gcaagagatt tcatatttgg gagagcatca
     2401 tgctggaact actttttgtg attggctttt ttgtcatgtt gatggtcacc ggtgtttcgt
     2461 tgctgggcat tatcgccgcg ctggttgtgg cgatggccat tatgttcctc ggcggtatgc
     2521 tggcattgat gattaagttg ctgccgtggt tactcctggc gattgcggtg gtgtgggtta
     2581 ttaaggcgat taaagcacca aaagtgccga aatatcagcg ttatgaccgc tggcgttact
     2641 aagggattgt gcggatgatc acaacctaag gttttatcct tagaacaaaa taggaattga
     2701 taatcaaatc tgtcactatt gcgcctctaa cagattcatc gtgctgtacc ctacatacag
     2761 ccgaactata aaaagaaagg gcttcccagg tggaagccct atttctttta tggaatcaac
     2821 aggctggaac cttgcgtcgc ccggctttcc agaatctcat gcgcacgctg cgcatccttc
     2881 agcggatatt tctgctgctc ggcgacatcg accttaatca caccgctggc aatcaaagag
     2941 aacagttcat tactggcctc ggttaattcc tcccgcgtgg tgatatagcc ttgcagggaa
     3001 gggcgtgtca catacaacga gcctttctga ttgagaatgc ctaagttcac accggtaacc
     3061 gcacctgatg agttgccaaa actcaccatt aagccgcggc gttgcaggca atccagcgac
     3121 cgttcccagg tgtctctgcc cacggaatcg tacaccacgc gcactttttt accaccggtg
     3181 atctctttta accgctcgac cagattctct tcacgatagt taataacctg ccacgcgccc
     3241 gcttttagcg cgctctgcgc tttttgcgcg gttcctacgg tgccgataag tttcgcgccc
     3301 agggcttttg cccactggca ggcaatcaag ccaacgccgc cagccgctgc gtggaacagg
     3361 aactgttcat cgggtttaat ttcataggtt ttgcgcagca gataataaac cgttaagcct
     3421 ttcaggaagg atgccgcagc ttgctcaaaa gaaattgccg caggcagaat cgccgcttta
     3481 tccgcaataa tgttatgcac agagctgtaa gcgcctaacg ccgactgcgc atagactaca
     3541 cgatcgcctg ccttaatatg ctttacacca ctgccgactt tactcacgat gcctgctgct
     3601 tcggtgccta atccgctggg tagcgatggc ggcgggtaaa ggccgctgcg gatatacgtg
     3661 tcgataaaat tgatgccgat ggctttattt tcgacctgga tttcattctc cgccggatcg
     3721 gcaggagtga actctacggc ttgaagtact tccgggccac cgtgcttgtg aaattcaatt
     3781 cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct gcttcatatg gtaaagtttc
     3841 gacccattct ttatctcggt aactccattc actatggcag gaaataaacc cttcaacaaa
     3901 cagcaggctg aaccccgcga acgcgatcca caagttgccg ggctgaaagt gcctccgcac
     3961 tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc tggataacga acgctgggat
     4021 gatgtagccg agcgtgtggt agcagacgat ttttacaccc gcccacaccg tcatatcttt
     4081 actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg atctgattac ccttgcggaa
     4141 tcgctggaac gccaggggca actcgatagc gtcggtggtt ttgcttatct ggcagagctg
     4201 tcaaaaaata cgccaagtgc ggcgaacatc agtgcctatg cggacatcgt gcgtgaacgt
     4261 gccgttgtcc gtgagatgat ctcggttgcg aatgagattg ccgaagccgg ttttgatccg
     4321 caggggcgta ccagcgaaga tctgctggat ctggctgaat cccgcgtctt taaaattgcc
     4381 gaaagtcgtg cgaacaaaga cgaaggaccg aagaacatcg ccgatgtgct cgacgcaacc
     4441 gtggcgcgta ttgagcagtt gtttcagcag ccacacgatg gcgttaccgg ggtaaacacc
     4501 ggttatgacg atctcaacaa aaaaaccgct ggcttgcagc cgtcggattt gatcatcgtc
     4561 gccgcgcgtc cgtcgatggg taaaacgaca tttgcgatga acctcgtcga aaacgcggcg
     4621 atgttgcagg ataaaccggt acttatcttc tcgctggaga tgccatcaga acagattatg
     4681 atgcgttctc tggcgtcgtt gtcgcgcgtt gaccagacta aaatccgtac cgggcagctg
     4741 gatgatgaag actgggcgcg catttccggc accatgggta ttttgctcga aaaacgcaat
     4801 atctatatcg atgactcctc cggcctgacg ccaacggaag tgcgttcccg cgcacgccgt
     4861 attgcccgtg aacacggcgg catcgggctt atcatgatcg actacctgca actgatgcgc
     4921 gtaccggcgc tttccgataa ccgtacgctg gaaattgcag aaatctcccg ctcgctgaaa
     4981 gcactggcga aagaattgaa cgtgccggtg gtggcgctgt cccagttgaa ccgttctctg
     5041 gaacaacgtg ccgacaaacg cccggtcaac tccgacctgc gtgaatctgg ctctatcgag
     5101 caggatgcgg acttgatcat gtttatctat cgtgatgagg tgtatcacga aaacagtgat
     5161 ttaaaaggca tcgcggaaat tattatcggt aaacaacgta acggcccaat cgggacggtt
     5221 cgcctgacct ttaacggtca atggtcgcgc ttcgacaact atgcggggcc gcagtacgac
     5281 gacgaataat aattatttta tgaattaggt aattaaagca aacatttatc aaggaacaca
     5341 aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg cgacacaacc tgcaacgtct
     5401 tcgtgaactg gctcctgcca gtaaaatggt tgcggtggtg aaagcgaacg cttatggtca
     5461 cggtcttctt gagaccgcgc gaacgctccc cgatgctgac gcctttggcg tagcccgtct
     5521 cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa cctgtactgt tactcgaagg
     5581 cttttttgat gccagagatc tgccgacgat ttctgcgcaa cattttcata ccgccgtgca
     5641 taacgaagaa cagctggctg cgctggaaga ggctagcctg gacgagccgg ttaccgtctg
     5701 gatgaaactc gataccggta tgcaccgtct gggcgtaagg ccggaacagg ctgaggcgtt
     5761 ttatcatcgc ctgacccagt gcaaaaacgt tcgtcagccg gtgaatatcg tcagccattt
     5821 tgcgcgcgcg gatgaaccaa gatgcggcgc aaccgagaaa caactcgcta tctttaatac
     5881 cttttgcgaa ggcaaaccag gtcaacgttc cattgccgcg tcgggtggca ttctgctgtg
     5941 gccacagtcg cattttgact gggtgcgccc gggaatcatt ctttatggcg tctcgccgct
     6001 ggaagatcgc tccaccggtg ccgattttgg ctgtcagcca gtgatgtcac taacctccag
     6061 cctgattgcc gtgcgtgagc ataaagccgg agagcctgtt ggttatggtg gaacctgggt
     6121 aagcgaacgt gatacccgtc ttggcgtagt cgcgatgggc tatggcgatg gttatccgcg
     6181 cgccgcgccg tccggtacgc cagtgctggt gaacggtcgc gaagtgccga ttgtcgggcg
     6241 cgtggcgatg gatatgatct gcgtagactt aggtccacag gcacaggata aagccgggga
     6301 cccggtcatt ttgtggggtg acggtttgcc cgtagaacgt atcgctgaaa tgacgaaagt
     6361 aagcgcttac gaacttatta cgcgcctgac ttcaagggtc gcgatgaaat acgtggatta
     6421 atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg tgaatgcctt atccggccaa
     6481 taaaatccta ccaattcaat aagttgatat tctttcatgc tcttataaag gtcgtgcctc
     6541 tggcggatgt acgtttgtca tgagtctcac tctgttgcta attgccggtc gcccctgaac
     6601 atccactcga tcttcgcctt cttccggttt attgtgtttt aaccacctgc ccgtaaacct
     6661 ggagaaccat cgcgtgtttc aaaaagttga cgcctacgct ggcgacccga ttcttacgct
     6721 tatggagcgt tttaaagaag accctcgcag cgacaaagtg aatttaagca tcggtctgta
     6781 ctacaacgaa gacggaatta ttccgcaact gaaagccgtg gcggaggcgg aagcgcgcct
     6841 gaatgcgcag cctcatggcg cttcgcttta tttaccgatg gaagggctta acagctatcg
     6901 ccatgccatt gcgccgctgc tgtttggtgc cgaccatccg gtactgcaac aacagcgcgt
     6961 agcaaccatt caaacccttg gcggctcagg ggcattgaaa gtgggcgcgg atttcctgaa
     7021 acgctacttc ccggaatcag gcgtctgggt cagcgatcct acctgggaaa accacgtagc
     7081 aatattcgcc ggggctggat tcgaagtgag tacttacccc tggtatgacg aagcgactaa
     7141 cggcgtgcgc tttaatgacc tgttggcgac gctgaaaaca ttacctgccc acagtattgt
     7201 gttgctgcac ccatgttgcc acaacccaac gggtgccgat ctcactaatg atcagtggga
     7261 tgcggtgatt gaaattctca aagcccgcga gcttatccca ttcctcgata ttgcctatca
     7321 aggatttggt gccggtatgg aagaggatgc ctacgccatt cgcgccattg ccagcgctgg
     7381 attacccgct ctggtgagca attcgttctc gaaaattttc tccctttacg gcgagcgcgt
     7441 tggcggactt tctgttctgt gtgaagatgc cgaagccgca ggccgcgtac tggggcaatt
     7501 gaaagcaaca gttcgccgca actactccag cccgccgaat tttggtgcgc aggtggtggc
     7561 tgcggtgctg aatgacgagg cattgaaagc cagctggctg gcggaagtag aagagatgcg
     7621 tactcgcatt ctggcaatgc gtcaggaact ggtgaaggta ttgagcacag agatgccaga
     7681 acgcaatttc gattatctgc ttaatcagcg cggcatgttc agttatacag gtttaagtgc
     7741 cgctcaggtt gaccgactac gtgaggagtt tggtgtttat ctcatcgcca gcggtcgcat
     7801 gtgtgtcgcc gggttaaata cggcaaatgt acaacgtgtg gcaaaggcgt ttgctgcggt
     7861 gatgtaatgc aggaaagcag gctggaatta cccagcctgc agtgaaatta aactgtcgtc
     7921 gctttcacac tttctttata gatgatttt
//
LOCUS       DAZFFG010000175         7884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFFG010000175 DAZFFG010000000
VERSION     DAZFFG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.176"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            117..767
                     /gene="csgD"
                     /locus_tag="ACHJ7J_004567"
     CDS             117..767
                     /gene="csgD"
                     /locus_tag="ACHJ7J_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO5002691.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQEHVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            772..1161
                     /gene="csgE"
                     /locus_tag="ACHJ7J_004568"
     CDS             772..1161
                     /gene="csgE"
                     /locus_tag="ACHJ7J_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO5002692.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1186..1602
                     /gene="csgF"
                     /locus_tag="ACHJ7J_004569"
     CDS             1186..1602
                     /gene="csgF"
                     /locus_tag="ACHJ7J_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO5002693.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1629..2462
                     /gene="csgG"
                     /locus_tag="ACHJ7J_004570"
     CDS             1629..2462
                     /gene="csgG"
                     /locus_tag="ACHJ7J_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO5002694.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2526..3017)
                     /gene="ycdZ"
                     /locus_tag="ACHJ7J_004571"
     CDS             complement(2526..3017)
                     /gene="ycdZ"
                     /locus_tag="ACHJ7J_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5002695.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3119..3673)
                     /gene="ycdY"
                     /locus_tag="ACHJ7J_004572"
     CDS             complement(3119..3673)
                     /gene="ycdY"
                     /locus_tag="ACHJ7J_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO5002696.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3697..4434)
                     /gene="ycdX"
                     /locus_tag="ACHJ7J_004573"
     CDS             complement(3697..4434)
                     /gene="ycdX"
                     /locus_tag="ACHJ7J_004573"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO5002697.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4489..5427)
                     /gene="ghrA"
                     /locus_tag="ACHJ7J_004574"
     CDS             complement(4489..5427)
                     /gene="ghrA"
                     /locus_tag="ACHJ7J_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO5002698.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESSLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5661..5748
                     /locus_tag="ACHJ7J_004575"
     tRNA            5661..5748
                     /locus_tag="ACHJ7J_004575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5695..5697,aa:Ser,seq:gga)
     gene            complement(5898..6740)
                     /locus_tag="ACHJ7J_004576"
     CDS             complement(5898..6740)
                     /locus_tag="ACHJ7J_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5002699.1"
                     /translation="MSDITISRLEVVNGHTDIICSTSVSHILAVRKTTLLQIDTLIRQ
                     LTEISAMTESIGGKTALDWAMKQDFRCGCWLMDKPDTAMKAITRNLDREIWRDLMQCS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEENILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSLQGKECYEDEMFIIKYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6837..7034)
                     /locus_tag="ACHJ7J_004577"
     CDS             complement(6837..7034)
                     /locus_tag="ACHJ7J_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5002700.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREGIRTLCQLQLLHQNR"
     gene            complement(7046..7537)
                     /locus_tag="ACHJ7J_004578"
     CDS             complement(7046..7537)
                     /locus_tag="ACHJ7J_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5002701.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINVVCDNGH
                     SLRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7534..>7884)
                     /locus_tag="ACHJ7J_004579"
     CDS             complement(7534..>7884)
                     /locus_tag="ACHJ7J_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5002702.1"
                     /translation="VREASRCPSPVTIWQTLLGRLLDQHYGLTLNDTPFADERVIEQH
                     IEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATGLMTRDNYR
                     TVNNITLGKHPQAK"
BASE COUNT         2047 a         1956 c         1865 g         2016 t
ORIGIN      
        1 atttagggct gatttattac tacacacagc agtgcaacat ctgtcagtac ttctggtgct
       61 tctattttag aggcagctgt caggtgtgcg atcaataaaa aaagcggggt ttcatcatgt
      121 ttaatgaagt ccatagtatt catggtcata cattattgtt gatcactaaa ccttctttgc
      181 aggcgacggc tctcttgcag caccttaaac aatcgctggc aattacagga aaattacata
      241 atattcaacg ttctctggac gatatatctt caggctctat tattcttctg gatatgatgg
      301 aagcggataa aaagcttatc cattattggc aggatacttt gagcaggaaa aacaacaata
      361 tcaaaatatt gttgctaaat acgcctgaag attacccgta ccgcgacatt gaaaactggc
      421 ctcatatcaa cggcgttttt tatgccatgg aggatcaaga acatgttgtc aatgggttgc
      481 aaggcgtcct gcgcggcgaa tgctacttta cgcaaaagct tgccagctac ctgattacgc
      541 attcgggtaa ctatcgttat aacagcacgg aatcagccct ccttactcat cgggaaaaag
      601 agatcctgaa taagctgcgt atcggcgcgt ctaataacga gatcgctcgt tcgttgttca
      661 tcagcgaaaa tacggttaaa acgcatcttt ataatctttt caagaagata gccgtaaaaa
      721 accggacaca agcggtttcc tgggcaaacg ataacctcag gcgataaagc catgaaacgt
      781 tatttacgct ggattgtggc ggcagaattt ctgttcgccg cagggaatct tcacgccgtt
      841 gaggtagaag tcccgggatt gctaactgac catactgttt catctattgg ccatgatttt
      901 taccgagcct ttagtgataa atgggaaagt gactatacgg gtaacttaac gattaatgaa
      961 aggcccagtg cacgatgggg aagttggatc actataacgg tcaatcagga cgttattttc
     1021 cagacttttt tatttccgtt gaaaagagac ttcgagaaaa ctgtcgtctt tgcactgatt
     1081 caaactgaag aagcactaaa tcgtcgccag ataaatcagg cgttattaag tacgggcgat
     1141 ttggcgcatg atgaattcta aataaaaaat tgttcggagg ctgcaatgcg tgtcaaacat
     1201 gcagtagttc tactcatgct tatttcgcca ttaagttggg ctggaaccat gactttccag
     1261 ttccgtaatc caaactttgg tggtaaccca aataatggcg cttttttatt aaatagcgct
     1321 caggcccaaa actcttataa agatccgagc tataacgatg actttggtat tgaaacaccc
     1381 tcagcgttag ataactttac tcaggccatc cagtcacaaa ttttaggtgg gctactgtcg
     1441 aatattaata ccggcaaacc gggccgcatg gtgaccaacg attatatcgt tgatattgct
     1501 aaccgcgatg gtcaattgca gttgaacgtg acagatcgta aaaccggaca aacctcgact
     1561 atccaggttt cgggtttaca aaataactca accgattttt aagccccagc ttcataagga
     1621 aaataatcat gcagcgctta tttcttttgg ttgccgtcat gttactgagc ggatgcttaa
     1681 ccgccccgcc taaagaagcc gccagaccga cattaatgcc tcgtgctcag agctacaaag
     1741 atttgaccca tctgccagcg ccgacgggta agatctttgt ttcggtatac aacattcagg
     1801 acgaaaccgg gcaatttaaa ccctacccgg caagtaactt ctccactgct gttccgcaaa
     1861 gcgccacggc aatgctggtc acggcattga aagattctcg ctggtttata ccgctggagc
     1921 gccagggctt acaaaacctg cttaacgagc gcaagattat tcgtgcggca caagaaaacg
     1981 gcacggttgc cattaataac cgaatcccgc tgcaatcttt aacggcggca aatatcatgg
     2041 ttgaaggttc gattatcggt tatgaaagca acgtcaaatc tggcggggtt ggggcaagat
     2101 attttgggat cggtgccgac acgcaatacc agctcgatca gattgccgtg aacctgcgcg
     2161 tcgtcaatgt gagtaccggt gagatcctct cctcggtgaa caccagtaag acgatacttt
     2221 cctatgaagt tcaggccggg gttttccgct ttattgacta ccagcgcttg ctggaagggg
     2281 aagtgggtta cacctcgaac gaacccgtta tgctgtgcct gatgtcggct atcgaaacag
     2341 gggtcatttt cctgattaat gatggtattg accgtggtct gtgggatttg cagaacaaag
     2401 cagaacggca gaatgacatt ctggtgaaat accgccatat gtcggttcca ccggaatcct
     2461 gacagataaa aaagccggaa ttatcatccg gcattttact gcttaagcga aagcgtgagt
     2521 gcccctcacg ctttattttt gatttcctgc tcacggtgcg ccgtctttgc actacgcgcc
     2581 gccagccaca ggccgctgtt tttcattgcg taaccaaata tcagcccaag cgccagcgaa
     2641 ggtaacacca gtttccagtc accctgcccg gcaaacgtcg cgcatgcgcc tataaacgtc
     2701 cccggaacaa atgaaagcag cagctgtttg gcctgaatac acatcagaaa cgcgacaata
     2761 ccggttatga cataaccgag aatttccaga tgtggtgcca gtgcactacc gtaaataatg
     2821 accatcgccc acaccacgcc actaagcagg gttgcagcgg agatcgccag ccctttcagc
     2881 ccaccttgcg ggcaagcaaa gtaggccgta cagcccagga agcccgccca gcttagtaag
     2941 cccagggaaa cagccaccca tccccagata ccggagagaa tgcctgttgt gattgcaata
     3001 gagagaagta tgttcatgcc gcgcatgata gcagaaaacg cggccatgaa tgagatcgag
     3061 cacacatttt atgcaacaac aaattatccg ttgcagtcat gagtgacgaa gatcacattt
     3121 actcttcaga atcctcttcc agctcgtccc acattgcact aatggcatca cgggttagcg
     3181 gtgccatggt gcgccagaaa ggcgtggttg catgggcctc cactttgcca aggaacgcgc
     3241 cacaccaggg taacagatac tcactgaaca gtgtttccag tgcttcgctc tcatcttccg
     3301 ttgactgatc ttccagccag gaagccgcaa gcagcaatgt gccgatgtga tcggctggcg
     3361 tatccgccag tggcatccct cgttcggaaa gaaaagcgcg cacttccgct tccgttgcgt
     3421 cctcaaccca tgcgctacga tatggcggca cggcacattc atcgccgata aacaacgcat
     3481 tgtaatcggc agagacttgc gtcatatcac aacttttctg taaacgcgtc agtaactcat
     3541 cctgctccag tggccagttc gcagccagtt tcccctcacg aatcagagta aacagcggca
     3601 ccagtaaagg atcttgtggt tggcggtaat agagcgaacc cagcacacga cagaggatag
     3661 aaaactcgtt cattaatata tttccattac tagtaattaa aggtctgcaa attccgcaat
     3721 cggtgccata ccgcgagatt caaggaagtt cagtaagcgg cgcggagaaa cattcaaaat
     3781 gcgctctggc ggaaaatcta ccgcgtcgag gattttaaga cactcttcaa attcccccat
     3841 ggtaaacgcg gtgtgagaat ccgagcctaa tgccacccaa cctccagcat cacgtaccgc
     3901 tgcggctacc gcacgacagt tgtcttcact gcccttgcgt gagtgtaaaa atgaggaatt
     3961 attgatttcc agcgccacct gatgtttcgc ggctgcttca gcaacggctt taacatctat
     4021 ttcatatttc ggatttccgg gatggcttat tatatgcaca ttgccgctgg cgatagtggc
     4081 gatcatcgct tgtgtattgg tcgctttgtc atgtggtgca aaaaccggct catgaaaacc
     4141 ggcaataatc agatccagcg agtcaaacat tttaccgctg caatcaattt caccatcaac
     4201 atttttaatg ttagcttcga tgccgcgcag gatccctacc ccatcaacca ctcgcggcca
     4261 gatacgcatg ttaatgaagt gccagtgatg cggcgcatct tccatatccg ggccatgatc
     4321 ggtgatcgca aaaagtttaa tgcccttttg tttggcctgg gcaatgtaat cactcaatgt
     4381 gctatatgca tgtgtgctgg caacggtatg catatgaagg tcgacgggat acataactct
     4441 ctcctgtatt caattcccgc caaggatagc aggaatcctg atgctttatt agtagccgcg
     4501 tgcgcggtcg acttgcccgc agaccctctc ccctttttcg agctgggcaa tagtgcgaga
     4561 aatgtactcc acagcttcag cgggacgggt aatcgcggcg acatgtggtg ttatcgtcac
     4621 gcgtggatgt tgccagagcg agctttcagg cggtaagggt tcacgattaa aaacatccag
     4681 cattgcacct ttaactttcc cgctatccag cgccgcgagc aggtcatctt ccacaacatg
     4741 aacaccacgc gccaggttga ggagatacgc gccatccggt aatttttcga gtaattgttg
     4801 attaataatg ccgacggttt caggagtatt cggtaacaaa ttaatcaata cccgacattg
     4861 gctcagaaat gcagacagtt cttcccgtcc ggcaaagctt tgcacgccag gccacgattt
     4921 acgggttcga ctccagcaac gcagcggaaa gcgccaggtt tgcagactct gagcaacttt
     4981 actgcccagt acgcctgcgc ccaaaatgcc gatggtaaaa tcttcccgat gatattcagg
     5041 cagcggttgc caatgcgaac tattttgctg gatgcgataa tcgtcaaaac gtcgaaacca
     5101 atgcagcacc tgactgacag catattcctg catttgctcg cccataccgg tatcttccag
     5161 gcgaaaaagt ggaacagaag ggttcagcat ttcagggtgt gcctgtagct tgctcaaaat
     5221 agaatcaaca ccggccccga gtgcgaacac cgctttaaga tcgcgccctg ccagcatttc
     5281 aacaggagga tgccagacta aagcataatc agcagagtca ttatctccgc ttttccatgc
     5341 tctgactctt gcctgaggaa tagctttgcg cagtgcctca atccaccatt gggtatcgaa
     5401 cgttgggtga taaaagatga tatccatact gactcccgaa aagcgttgtg cgaaatttat
     5461 tcgcacttat cgttatgatc tacaaagacc accagcataa caaatccgtg gtcggtggca
     5521 aaaaaagcag atttcgctta ttaaaaccac atattgattg aagtttgaat aaacgcgcga
     5581 ttttttaaaa aagtttgttg acctcaggtc atgatttccc taaattagcg cccgttccag
     5641 caagacagga acgacaattt ggtgaggtgt ccgagtggct gaaggagcac gcctggaaag
     5701 tgtgtatacg gcaacgtatc gggggttcga atccccccct caccgccaga ttacaagcgc
     5761 atgttattgt ttaacatgcg cttttttatt tatccttcca gacagtatgc caccgggtat
     5821 gccatctgaa accggtttat atgccccacg atcatatctt atcggacaat ttccgatctt
     5881 tgagcatact ggcaaagcta tactgccagc atgtcaggat agtgttttgc gataatatca
     5941 ttcagtctgt caaccagttc tggcttcctg aacgtgatgt gcgcagagcc cttcttaaag
     6001 tatttaataa tgaacatctc atcttcatag cattcttttc cctgaagaga ccggatgtga
     6061 tcgtccagat gaatagttat gttttccctg ttgtcgggga tcggtttgcc gctgaacaga
     6121 tgcagcattc tttccaggtc ggcaagtcta tctgcctgtt gcccggtgat aagatgcaat
     6181 ccccaccggt cccacctcac cagattgttg acgataattt tattgccaaa tttacagggg
     6241 caattggttt tgtaattcca gctcaaccct ctgaagaggt tgatcactcc tcgctcaaac
     6301 acttcatcct tattctggtg tagctgctca aacgtactca gaatattctc ttcactgatt
     6361 tccggaaaat tatcgtactc cagtgatcgg taccatgtat cgcgggcctg tgcatccatt
     6421 aaggaaagca tcccggagca ttgcatcagg tcacgccaga tttcgcgatc gagattgcgg
     6481 gtgatagctt tcattgctgt atcaggttta tccatgagcc agcagccgca gcgaaaatcc
     6541 tgtttcatgg cccagtccag tgcggtttta ccgccaatac tttctgtcat tgctgatatt
     6601 tcagtcagtt gccggataag tgtgtcgatt tgcagcagtg tagtctttcg cacagccaga
     6661 atgtggctga ctgaggtgga gcagataatg tccgtatgtc cgttgaccac ttccaggcgg
     6721 gagattgtga tatctgacat catcgttttt ccttttggtt tataaacgaa aacgccagcc
     6781 ctgttcaggc tgacgtcatt ggaatgaagt ctggtgagtg agatgatgag taagactcat
     6841 cggttctggt gcagaagctg cagttggcag agggtacgga taccctccct ggcctgttcg
     6901 atacagggca acagtgctgc cgaatctgtt ttatcctcat cgttgtcaaa gataattccc
     6961 gattcgcagt cgatattgtc ctgcagccac gcaatcagaa tatccagtgc ggtttccgtg
     7021 gttaatgatt tcatgttgtt catggttacc agtcgaaagt gggtaaaccc ggcaggcatt
     7081 cagcgctggc atccagatga atgagactga cgccataacg tcggatgagt gtgacaacca
     7141 gacggcggaa cgtttttgac aggcccaggc gtttaagccg cagaaccggg tatgaccacg
     7201 catcggtacg taacagatat ccgctgcctg taaaatgaat ccattctgat tcgccggtgt
     7261 cattcgtctg atgcgacagc gagtacagcc aggcgttgtc ttttgccgtg atatgcgcgg
     7321 tactgcagcg tatgccggtc agggcagcaa atggtggcgt gcagtctgtt gaggctcggt
     7381 cagattcatc aacaacacga agggagtgac cgttatcgca gaccacgtta atcagttcag
     7441 caagctcaat accatcgacg tcaacgacaa tgcgccccat gttcagtgcc tgtacgttaa
     7501 cgctgtcggc ttccagcgtc agggccagtt tcatcatttc gcctgcggat gcttacccag
     7561 ggttatgtta tttaccgttc tgtaattgtc gcgggtcatc aggccggtcg ccctgcgagc
     7621 ccggaggata tcgatgctgt ttattaactg agagcgggta caggcgctga atcccggctg
     7681 gtcggtacgc accagcgcgt atttttccac gagaaagttc accgcatcac acagtgaaat
     7741 acctgcctca atatgctgct caatcacacg ttcatcagcg aacggtgtgt cattcagcgt
     7801 gaggccgtag tgctggtcca gcagacggcc aagcagtgtc tgccagatgg tgacgggaga
     7861 cgggcagcgc gatgcctccc gtac
//
LOCUS       DAZFFG010000176         7875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFFG010000176 DAZFFG010000000
VERSION     DAZFFG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.177"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            396..938
                     /gene="ecpR"
                     /locus_tag="ACHJ7J_004580"
                     /gene_synonym="matA"
     CDS             396..938
                     /gene="ecpR"
                     /locus_tag="ACHJ7J_004580"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO5002703.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKNKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            1013..1600
                     /gene="ecpA"
                     /locus_tag="ACHJ7J_004581"
                     /gene_synonym="matB"
     CDS             1013..1600
                     /gene="ecpA"
                     /locus_tag="ACHJ7J_004581"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO5002704.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1658..2326
                     /gene="ecpB"
                     /locus_tag="ACHJ7J_004582"
                     /gene_synonym="yagY"
     CDS             1658..2326
                     /gene="ecpB"
                     /locus_tag="ACHJ7J_004582"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO5002705.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2352..4877
                     /gene="ecpC"
                     /locus_tag="ACHJ7J_004583"
                     /gene_synonym="yagX"
     CDS             2352..4877
                     /gene="ecpC"
                     /locus_tag="ACHJ7J_004583"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO5002706.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4867..6510
                     /gene="ecpD"
                     /locus_tag="ACHJ7J_004584"
     CDS             4867..6510
                     /gene="ecpD"
                     /locus_tag="ACHJ7J_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO5002707.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPASEFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINTCQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWRPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLLIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6479..7189
                     /gene="ecpE"
                     /locus_tag="ACHJ7J_004585"
     CDS             6479..7189
                     /gene="ecpE"
                     /locus_tag="ACHJ7J_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO5002708.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7502..7831
                     /gene="ykgJ"
                     /locus_tag="ACHJ7J_004586"
     CDS             7502..7831
                     /gene="ykgJ"
                     /locus_tag="ACHJ7J_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5002709.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
BASE COUNT         2066 a         2011 c         2016 g         1782 t
ORIGIN      
        1 agcgcattaa gactattcct aacacttcag ggcaaagttc ctgaccaata taaaatgcaa
       61 gtaagaaatg aacgttatat tgcaaataac cttatgaaac taaatgtctt tttcttctta
      121 tcaaaaaagc aatattttca gtttttctaa atattgactt aaccattgaa ttccttttcc
      181 gttcacatat tgacactcat cgggaaaaaa acataaattt aagcccaatc gaaaataatt
      241 aaacttaatc tcgtttaacc tttattgata tgtactacgt atcttattta cttccggttt
      301 actaaggaaa ctgaatgcac ctgtaaaaat tacaggtttg gaaagtagtg acatggcaaa
      361 gtgattacag cagggactat gaggttaaaa accatatgga atgtcaaaac cgttctgata
      421 aatacatctg gtctccccat gacgcctact tctataaagg actatctgaa ctgattgtgg
      481 atatcgacag attaatttat ctatcgttgg agaaaattag aaaagatttc gtgtttatca
      541 atctcaatac ggattcttta tctgaattta taaaccgtga taatgaatgg ttatccgcgg
      601 taaaggggaa acaggtcgta ttgattgcgg ccagaaagtc agaagcctta gcaaattatt
      661 ggtattacaa cagcaatatt aggggcgtgg tatacgctgg actgagtcgt gatattagaa
      721 aagaactggc ctatgtgatt aatggcaggt tcctgagaaa agatattaag aaaaataaaa
      781 tcactgaccg ggaaatggaa attatccgca tgacggccca gggaatgcaa cctaaatcga
      841 ttgccagaat tgaaaattgt agtgtgaaga cagtgtatac ccatcggcgt aatgctgagg
      901 ccaagctgta ctcaaaaata tataagttgg ttcagtaaac tccaggcaag ttagttttaa
      961 aaaatgactc actgggacat cacgtcctca attcaactcg ggaagaaatg caatgaaaaa
     1021 aaaggttctg gcaatagctc tggtaacggt gtttaccggc atgggtgtgg cgcaggctgc
     1081 tgacgtaaca gctcaggctg tagcgacctg gtcggcaaca gccaaaaaag acaccaccag
     1141 taagctggtt gtgacgccac tcggtagcct ggcgttccag tatgccgaag gcattaaagg
     1201 ttttaactca cagaaaggtc tatttgacgt ggctatcgag ggtgactcaa cggctaccgc
     1261 ctttaaactg acctcacgtc ttatcaccaa cacattaacc cagttggata cctcaggttc
     1321 cacactgaat gtgggcgtgg attataacgg cgcggcagtc gaaaaaactg gcgataccgt
     1381 gatgatcgat accgccaacg gcgtactggg cggcaacctt agcccgctgg ctaacggtta
     1441 caatgccagc aatcgtacca ccgcacagga tggtttcacc ttcaccatca tcagcggcac
     1501 caccaatggt accaccgcag taaccgacta cagcactcta ccggaaggca tctggagcgg
     1561 cgacgttagc gtacagttcg acgcgacctg gaccagttaa tctctctgat gtaccagcag
     1621 gggtagcccc cctgctttat tcctggacgg aattgttatg aaaaagcacc ttctgcttct
     1681 cgctctgctg ttatccggaa tatctccggc ccaggcgctg gatgtcggcg atatatcatc
     1741 gtttatgaac agtgacagca gcacgctgag caaaacgatc aaaaacagta ccgacagtgg
     1801 tcgcctcatc aatatccgcc tcgaacggct ctcttcaccg cttgacgacg ggcaggttat
     1861 ctcaatggac aagccggacg agttgctact cactcccgcc agcttgctgc tacccgccca
     1921 agccagcgaa gtgatccgct tcttctataa gggacccgca gatgaaaaag agcgctacta
     1981 ccgcattgtc tggtttgatc aggccctcag tgatgcgcag cgcgataatg ccaaccgcag
     2041 cgctgtggcc actgcttccg cccgcatcgg caccattctg gtcgtcgcac cccgccaggc
     2101 aaactaccac tttcagtacg ccaacggctc cctgacaaat acaggaaatg cgacgctgcg
     2161 gatcctcgcc tacggcccct gcctgaaagc cgccaatggt aaagagtgta aagagaatta
     2221 ctacctgatg ccgggcaagt cgcgtcgttt tacccgcgtg gacacggcgg ataacaaagg
     2281 acgggttgca ctttggcagg gtgataagtt cattcccgtg aaatagatag ctgtgcagat
     2341 ggataacgac aatgccttta cgacggttct ccccaggact gaaagcccag tttgccttcg
     2401 gcatggtctt tttgttcgtt cagcccgatg ccagcgctgc tgacataagt gcgcagcaaa
     2461 taggtggggt gattattccg caggccttca gtcaggcgct ccaggacggc atgagcgtcc
     2521 cgctttatat tcatctcgcc ggtagccagg gtcgccagga cgatcagcga atcggcagcg
     2581 cttttatctg gctggatgat ggacagctac gcatccggaa aatacagctg gaagagagtg
     2641 aagataacgc cagtgtcagc gaacaaactc gacagcagct gatggctctg gcgaacgccc
     2701 cgttcaatga ggcccttacc atccccctga ctgacaacgc gcagctggat ctcagcttgc
     2761 gccaactgct gctgcagctg gtggtcaagc gcgaagcgct gggcactgta ctacgctcac
     2821 gtagcgaaga catcgggcag tccagtgtta acaccctcag cagtaatctg agctataact
     2881 tcggcgtcta taacaaccag ttgcgtaacg gcgggagcaa cacatccagc tatctgtcgc
     2941 tgaataacgt tactgcgctg cgcgaacatc atgtggtgct cgacggctcg ctgtacggga
     3001 tcggtagcgg tcaacaggac agtgaattat ataaagcgat gtatgaacgc gattttgccg
     3061 gtcaccgatt tgccggtgga atgctcgaca cctggaactt gcagtcctta gggccgatga
     3121 ccgccatttc agcagggaag atttacggcc tttcctgggg aaaccaggcc agctccacca
     3181 tcttcgacag cagccagtca gccacgccag tgatcgcctt tttaccggcg gcgggcgaag
     3241 tacatctcac ccgtgatggg cggttactaa gcgttcagaa cttcaccatg ggcaatcatg
     3301 aagtggatac ccggggtcta ccgtacggta tttacgatgt ggaagttgag gtgatcgtta
     3361 acggtcgcgt gatcagcaaa cgtacccagc gggtcaataa gctgtttagc cgggggcgcg
     3421 gcgtcggtgc accactggcg tggcaggtat ggggcggtag ctttcatatg gatcgctggt
     3481 cggaaaacgg gaaaaagacg cgaccagcta aagagagttg gctggcaggt gcttcgacct
     3541 ccggctcact gagtacgctg agctgggcgg caacgggata tggatacgat aatcaggcgg
     3601 tgggtgaaac ccgtctgacg ctgccgcttg ggggggcgat caacgttaac ctgcaaaata
     3661 tgctggccag tgacagctca tggagcaaca tcgccagcat cagcgccact ctaccgggag
     3721 gctttagttc gctgtgggtt aaccaggaaa aaacccgcat tggcaatcaa ttgcgacgta
     3781 gcgatgccga caaccgtgct atcggcggca cactcaacct gaactcactg tggtcgaagc
     3841 tgggcacatt cagcatcagc tacaatgatg accgccgtta caacagccat tattacacgg
     3901 cagattacta tcaaaatgtc tacagcggta cctttggttc gcttggcctg cgggccggta
     3961 ttcagcgcta taacaacggc gacagcaacg ccaatacagg gaaatatatc gctctcgatc
     4021 tctcgctacc actgggcaac tggtttagcg cagggatgac tcatcaaaac ggctacacca
     4081 tggcaaacct gtcagcacgc aagcagtttg atgaagggac tattcgcact gttggtgcca
     4141 atctgtcacg agccatctcc ggcgataccg gtgatgacaa aaccctcagc ggtggggcgt
     4201 atgcacagtt cgacgctcgt tacgccagcg gaacgctgaa cgtcaatagc gcggcggacg
     4261 gctacgtcaa taccaacttg accgccaatg gcagcgtcgg ctggcagggt aaaaacattg
     4321 ctgccagcgg gcggactgat ggcaacgctg gggtgatatt caacaccggg ctggaggacg
     4381 acggtcagat cagcgccaaa atcaacgggc ggattttccc acttaacggc aagcgtaact
     4441 atctcccgct ctctccctat ggaagatatg aggtggagtt acagaacagc aaaaactccc
     4501 tcgacagcta cgatatcgtc agcggccgca aaagtcatct gactctctat ccaggcaatg
     4561 tcgctgtcat tgagccagag gtgaagcaga tggttaccgt ctccggtcgt atccgtgcgg
     4621 aagacggcac actgctggct aacgcacgga ttaacaacca tatcggccga acccgaaccg
     4681 atgaaaacgg cgagtttgtc atggacgtgg ataagaaata ccccactatc gattttcgct
     4741 acagtggcaa taaaacctgc gaagtggctc tggaactcaa ccaggcgcgc ggtgccgtct
     4801 gggtcggtga tgtggtctgc agcggcctct catcgtgggc ggcggtgacg cagacaggag
     4861 aagagaatga gagttaacct actaataacg atgataattt tcgcgctaat ctggccagta
     4921 actgcgctca gagcggcagt gagcaaaaca acctgggcgg atgcaccggc aagcgagttt
     4981 gtgtttgtcg aaaacaactc agacgacaac tttttcgtca cgcctggcgg ggcgctggat
     5041 ccgcgcctga ccggtgccaa ccgctggacc ggtttaaaat acactggctc aggaaccatt
     5101 tatcaacaaa gcctcggcta cattgataac ggttacaaca ccggcctcta taccaactgg
     5161 aagtttgata tgtggctgga aaattcgccg gtttcatctc ctttaactgg cttacgctgc
     5221 atcaactggt acgcagggtg taatatgacc accagtctta tcctgccgca aaccaccgac
     5281 gccagtggat tttatggcgc gacggtgacc agcggcggcg cgaagtggat gcacggcatg
     5341 ttgtcagacg cgttttacca gtatatgcaa caaatgcccg tcggcagcag ctttacaatg
     5401 accatcaata cctgtcagac ctctgtgaac tatgacgcca gcagcggcgc gcgttgtaag
     5461 gatcaggcct ccggcaactg gtatgttcgc aacgtcaccc atacgaaagc agcaaatctg
     5521 cggttgataa acacccattc gctggcggag gtgtttatca acagcgacgg agtaccgact
     5581 ctgggcgaag ggaacgccga ctgccggacg caaaccatcg gcagcctttc aggattaagt
     5641 tgtaagatgg tcaactacac cctgcaaaca aacggactca gcaacacctc aatccatata
     5701 ttcccggcga tcgccaactc gtcgttagcc tcggccgtcg gggcgtacga tatgcagttc
     5761 agtctgaatg gcagttcatg gagaccagtg agcaataccg cctattacta caccttcaac
     5821 gagatgaaga gcgcagactc gatctatgtt ttcttctcga gcaacttctt taagcagatg
     5881 gtgaacctgg gaatcagcga tatcaacacc aaagatctat tcaactttcg ctttcagaac
     5941 actacatcac cggagtctgg ctggtatgaa ttttctacct ccaacacgct acttatcaaa
     6001 ccccgtgatt tcagcatcag tattatctcc gatgaatata ctcagacacc gtcgcgggag
     6061 ggatatgtcg gcagcggcga gtcggcactc gatttcggct atatcgtaac caccagcggt
     6121 aaaacagctg ccgacgaagt gctgatcaag gtgaccggac ccgcgcaggt gattggcggg
     6181 cgctcctatt gtgtcttcag ctccgatgac ggtaaggcga aagtaccgtt cccggcgacg
     6241 ctttccttta ttacccgcaa cggagctaca aaaacctacg atgccgggtg cgatgatagc
     6301 tggcgggata tgaccgatgc gctgtggctg accacaccgt ggactgatat ctctggcgaa
     6361 gtggggcaga tggataagac cacagtcaaa ttttcgattc caatggataa cgccatttcg
     6421 ctgcgtacgg tagatgataa cggctggttt ggcgaagtca gcgcttcagg agaaattcat
     6481 gttcaggcga cgtggcgtaa cattaactaa ggccctgctg acagcggtct gtatgctggc
     6541 ggcacctttg acacaggcga tttcggtcgg caatctgaca ttttcgctgc cgtccgagac
     6601 tgactttgtc agcaaacgtg tagtgaataa caacaaaagc gcacggatat accgtattgc
     6661 catcagtgct atcgatagcc cgggcagcag tgaattgcgc acccgaccgg tggatggtga
     6721 actgcttttc gccccccgcc agctggcgtt gcaggctggt gagagcgagt attttaaatt
     6781 ttactatcat ggtccacggg ataaccgcga gcgctactac cgggtctcat ttcgcgaggt
     6841 ccccactcgc aaccagacaa gacgcagccc aaccggcggc gaggtcagca cggagccggt
     6901 ggtggtgatg gataccattc tggtagtacg accacgtcag gttcagttta aatggtcctt
     6961 cgacaaggtg acaggaacgg taagtaacac tggcaacaca tggtttaagc tactgattaa
     7021 gccaggatgt gattcgaccg aagaggaagg cgatgcctgg tatctacgtc cgggagacgt
     7081 ggttcatcag cctgagttac gtcagccggg gaatcactat ctggtctata acgacaaatt
     7141 cattaagatt agcgattcct gtccggctaa gcccccttcg gcggactgat acttatccat
     7201 cggtcgaggg aagaattcca cctcagagac tcaaaaatcg tttaaatgat gtggaagcga
     7261 tcgtcaatat cacctgtaac gatgggcttt cccatatgca caccatagtg ttcaggagac
     7321 acggaaaggt atcaggctgc ctgctaacga gtggtttatt cacgaattag tacgtaaaat
     7381 cggtaacggc tggaaatcat tcaatactca cactatcgaa agtacaccag ccaaccgcgg
     7441 tacgttctta catacgatgt accgctgttc tctttacgat ttttagctgt actggtgaat
     7501 tatgagcaat ctgaatccat gcatgacgtg tggtgcctgt tgtgcatttt tccgcgtctc
     7561 tttctactgg gccgaagccg acgatgctgg cggcactatt cccgccaggc tcactgaaca
     7621 aatatcccct tttcaccgat gcatgagcgg taccaatcag aaaaaccccc gatgtattgc
     7681 ccttgcagga accccgggca aaaatgcctg ctgcacgata tataaaaatc gatcgtccac
     7741 gtgcagagaa ttcgccatgt ctggtgagaa cggagtcgtc aatgaggctt gcaatcgtgc
     7801 aagggctaaa tacgggctga caccgttata aatatacaat aattatttgc actgccccgc
     7861 cagctgtaat agcgg
//
LOCUS       DAZFFG010000177         7815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFFG010000177 DAZFFG010000000
VERSION     DAZFFG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.178"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(201..464)
                     /gene="cedA"
                     /locus_tag="ACHJ7J_004587"
     CDS             complement(201..464)
                     /gene="cedA"
                     /locus_tag="ACHJ7J_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO5002710.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            647..2908
                     /gene="katE"
                     /locus_tag="ACHJ7J_004588"
     CDS             647..2908
                     /gene="katE"
                     /locus_tag="ACHJ7J_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO5002711.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2955..3713)
                     /gene="chbG"
                     /locus_tag="ACHJ7J_004589"
     CDS             complement(2955..3713)
                     /gene="chbG"
                     /locus_tag="ACHJ7J_004589"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO5002712.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFLRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3726..5078)
                     /gene="celF"
                     /locus_tag="ACHJ7J_004590"
                     /gene_synonym="chbF"
     CDS             complement(3726..5078)
                     /gene="celF"
                     /locus_tag="ACHJ7J_004590"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5002713.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELSVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5185..6027)
                     /gene="chbR"
                     /locus_tag="ACHJ7J_004591"
     CDS             complement(5185..6027)
                     /gene="chbR"
                     /locus_tag="ACHJ7J_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO5002714.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6035..6385)
                     /gene="chbA"
                     /locus_tag="ACHJ7J_004592"
     CDS             complement(6035..6385)
                     /gene="chbA"
                     /locus_tag="ACHJ7J_004592"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO5002715.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6436..7794)
                     /gene="chbC"
                     /locus_tag="ACHJ7J_004593"
     CDS             complement(6436..7794)
                     /gene="chbC"
                     /locus_tag="ACHJ7J_004593"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO5002716.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2120 a         2038 c         1734 g         1923 t
ORIGIN      
        1 actatcaaaa atagctcatc atgattagca aaacttaacc attttaaaat aaataaacaa
       61 ttaaagaaaa aagatcactt atttatagca atagatcgtc aaaggcagct ttttgttaca
      121 ggtggtttga atgaatgtag caatgaaata caaaatttca ggccatgtaa ctcccggcaa
      181 aaccgggagg tacgtaatcc ttactcagtc acttcccctt cctggcggat ctgatttgcc
      241 caacgctggg cggattcagg cacagtaaac gccggtgagc gcagaaatga ctctcccatc
      301 agtacaaacg caacatattt gccacgcagc atccagacat cacgaaacga atccatcttt
      361 atcgcatgtt ctggcggcgc gggttccgtg cgtgggacat agctaataat ctggcggttt
      421 tgctggcgga gcggtttctt cattactggc ttcactaaac gcatattaaa aatcagaaaa
      481 actgtagttt agccgattta gcccctgtac gtcccgcttt gcgtgtattt cataacaccg
      541 tttccagaat agtctccgaa gtgggatctg gctggtggtc tatagttaga gagttttttg
      601 accaaaacag cggccctttc agtaataaat taaggagacg agttcaatgt cgcaacataa
      661 cgaaaagaac ccacatcagc accagtcacc actacacgat tccagcgaag cgaaaccggg
      721 gatggactca ctggcacctg aggacggatc tcatcgtcca gcggctgaac caacaccgcc
      781 aggtgcacaa cctaccgccc cagggagcct gaaagcccct gatacgcgta acgaaaaact
      841 taattctctg gaagacgtac gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg
      901 cgtgcgcatc gccgacgatc aaaactcact gcgtgccggt agccgtggtc caacgctgct
      961 ggaagatttt attctgcgcg agaaaatcac ccactttgac catgagcgca tcccggaacg
     1021 tattgttcat gcacgtggat cagccgctca cggttatttc cagccatata aaagcttaag
     1081 cgatatcacc aaagcggatt tcctctcaga tccgaacaaa atcaccccag tatttgttag
     1141 gttctctacc gttcagggtg gtgctggctc tgccgatacc gtgcgtgata tccgtggctt
     1201 tgccaccaaa ttctataccg aagagggtat ttttgacctt gttggcaata acacgccaat
     1261 cttctttatc caggatgcgc ataaattccc cgattttgtt catgcggtaa aaccagaacc
     1321 acactgggca attccacaag ggcaaagcgc ccacgatact ttctgggatt atgtttctct
     1381 gcaacctgaa actctgcaca acgtgatgtg ggcgatgtcg gatcgcggca tcccccgcag
     1441 ttaccgcacc atggaaggct ttggtattca taccttccgc ctgattaatg ccgaagggaa
     1501 ggcaacgttt gtacgtttcc actggaaacc actggcaggt aaagcctcac tcgtttggga
     1561 tgaagcacaa aaactaaccg gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc
     1621 cattgaagca ggcgattttc cggaatacga actgggcttc cagttgattc ctgaagaaga
     1681 tgaattcaag ttcgacttcg atcttctcga tccaaccaaa cttatcccgg aagaactggt
     1741 gcccgttcag cgtgtcggca aaatggtgct caatcgcaac ccggataact tctttgctga
     1801 aaacgaacag gcggctttcc atcctgggca tatcgtgccg ggactggact tcaccaacga
     1861 tccgctgttg cagggacgtt tgttctccta taccgataca caaatcagtc gtcttggtgg
     1921 gccgaatttc catgagattc cgattaaccg tccgacctgc ccttaccaca acttccagcg
     1981 tgatggcatg catcgtatgg ggatcgacac taacccggcg aattacgaac cgaactcgat
     2041 caacgataac tggccgcgcg aaacaccgcc gggaccgaaa cgcggcggtt ttgaatcata
     2101 tcaggaacgc gtggaaggaa ataaagttcg cgagcgcagt ccatcttttg gcgaatatta
     2161 ttcccatccg cgtctgttct ggctaagtca gacgccattc gagcagcgtc atattgtcga
     2221 tggtttcagt tttgagttaa gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga
     2281 ccagctggcg catattgatc tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga
     2341 actgactgac gaccagctga atatcacccc acctccggac gtcaacggtc tgaaaaagga
     2401 tccatcctta agtctgtacg ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat
     2461 tttacttaat gatgaagtga gatcggcaga ccttctggcc attctcaagg ccctgaaggc
     2521 caaaggcgtt catgccaaac tgctctactc ccgaatgggt gaagtgactg cggatgacgg
     2581 tacggtgttg cctatagccg ctacctttgc cggtgcgcct tcgctgacgg tcgatgcggt
     2641 cattgtccct tgcggcaata tcgcggatat cgctgacaat ggcgatgcca actactacct
     2701 gatggaagcc tacaaacacc ttaaaccgat tgcgctggcg ggagacgcgc gcaagtttaa
     2761 agcaacaatc aaggtcgctg accagggtga agaagggatt gtggaagctg acagcgccga
     2821 cggtagtttt atggatgaac tgttaacgct gatggcagca caccgcgtgt ggtcacgcat
     2881 tcctaagatt gacaaaattc cggcgtaaat atggataagg tcacgtcccc tggggagtga
     2941 ccttaccacc ttacttacac atcaagataa ctacccaacc gataaccacg ctcagcaatt
     3001 gcatatttca acgacgctga tgtcagcact tccagctccg ttaaccgtag gaaacagtaa
     3061 gcactctggc gaatcgtatt atcgacaaac gccggatggc acatgacttc cagcgagcgt
     3121 tccccgcgat ggctggaatc atcaagcact tgcaggaaca gtgcctcgct aatctcttca
     3181 ccataaaacg cgctactgaa tccgtgcgaa ctgcgcagat tagccggtaa atcaccagcg
     3241 ttagatagcg gctgacggtc gatgcgtaac gcaatcccct cttcagccgc aaacctggca
     3301 acaataggga aaatctgcgg gaacatatgc acatgatgat ggctatcaag atgcgtaggt
     3361 ttgcgtccaa atagctcaat gaaacgcaaa tactgactgg caagctcctg agtaatttct
     3421 tccagcggta aagcatcttc ttctgccaac tgccagatcc attttcccag cacaccatcg
     3481 cgggttaacc ccggcatagc tgtcagtggc ttgcccatag taaggacaaa gtgcatccct
     3541 atggccagac tcggttcatc acgactcaac tgcaccgcat ggtcaatagc ctgcccattc
     3601 accagcgccg tcgtcgatgt gacaatccca ttgcgacagg cctcgataat gccgtagttc
     3661 tgacctttgc ttaagccaaa atcatcggca ttaacaatca gtaagcgttc cataatcagc
     3721 ctcggttaat gtgctttttt aagctctgcg atgcagtcgg caaaatttgg cagccatttc
     3781 tcgtgcgcca gaatcatctc gcgtgccagc agctcagcat cgcgatcgga atgcaccaac
     3841 ggactaaggt ttagcgccag taacacatcg ttaaattctc cgctaagtgc ggcgttgctg
     3901 gcagcaatct cgaagccttt aatggtgtga atcagcccca tcactttatc atcgaaatgc
     3961 gtaatgcgcg gatgtggcgt cgcgccatcg cgccccagcg tacaggtcat ttctaccgcc
     4021 cagtctgccg gaatattatc aatatgccca tgatgcggga tattaacgta atgttctgct
     4081 tgcttgtcgt tgtagatagc gttgatcact tcgcacgctg catcagagta ataagcccca
     4141 ccgcgctgtt ccagttcttt cggcttaact ttcaactccg ggtttttata cagctcaaaa
     4201 agttgtttct cgactttctg tactacctgc gctcgtgcgc cgcctttgta gtattcgccc
     4261 atttcaatag ccagcatctc tttttgcttg aagtaataaa gcaaataaga acacggcaac
     4321 agattcagag aacgaattaa gccttcgcta aatggcagat cgaaaatatt tttaacgcca
     4381 gatgctttta actgcccgga tgccacacca tcaagtaatt cggcaaagcg agatttgcca
     4441 tttaccagca catccttaat gaacaccata tggttgaggc cgaacagatc gatagataaa
     4501 tcatcgctgt ctttcagcat cagaacatcg cgaataaaca tcttcatgcc gatcggaata
     4561 ttacacacgc cgataaagcg tttaaatccg gtatgacgat aaacggcttc agtgaccatt
     4621 cccgccgggt tagtgaagtt aatcacccat gcattcggac aaagttcttc gacatctttt
     4681 acgatgtcaa aaatcaccgg aatggtacgc agacctttaa acagaccgcc cgcgccgttg
     4741 gtttcctgac caagataacc atgacttaat ggaatacgtt catccagttc gcgcgccggt
     4801 aattggccta cgcgcagttg ggtagtaacg aaatcagcat ctttcaatgc ttcgcggcga
     4861 tccagcgttt tataaagctt catcgggacg ccagcgttat caatcatccg ttggcagaga
     4921 tcgaaaataa tatccagttt agctttgcca ccttcgacat ccaccagcca taattcgctg
     4981 accgacaatt cgtgataacg cttaataaat ccttccagta actccggggt atagctgctc
     5041 ccgccaccaa tagtgacgac ttttaatttc tggctcataa tttctccctt cagtacagaa
     5101 tactgatatc tggcatatct gccccccccc ggacataaat aatccagcaa caggacagat
     5161 atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct tacgatagct
     5221 cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata
     5281 accggcctca aacgcaatat ccgtcactga atagttggtc atttccagtt gttttttggc
     5341 aaaattaata cggatttcat taataatctg cattggcgtt ttgccataat atcgttgagt
     5401 cgcccgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga
     5461 ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc actgcggtac
     5521 atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac
     5581 catctcaaca aactcttcca gccctgtttc gcggaaattc aatgaagaaa tcactgtttc
     5641 cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagctacaa agcaataagg
     5701 caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt tcaatatacg
     5761 cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc
     5821 gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac
     5881 cagagtaaat tcataatagt cgtgctgatg cagtccgctg atactctcag ttttgttata
     5941 gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc
     6001 cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct
     6061 caattaattc ggtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt
     6121 ggaccagcac cagactcact ttcatcttac cttcgcccgc atcgccttca atcagtttcg
     6181 tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg gcttttgctg
     6241 cggcaaaatc gccctgcttc gcctgtttca gtgccgcata cgccaggctg cgcgcttgtc
     6301 cggagttgat gataagcccc atcaccactt cttccagctc ttcagcttcc gtttgcgtat
     6361 cgggaatatt atcgagatcc atcatacatc gttcctctct attcttaccg gcacgattac
     6421 ccgtaccggc atcgattaaa atttcagggc gttagcgata tcttcttcgc tctcttcttt
     6481 atcaatcgcg ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaacgttgc
     6541 gataccaagg ttgaagagtg caaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
     6601 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaataatgcc
     6661 catgtagtac gctgccaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
     6721 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
     6781 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
     6841 aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
     6901 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
     6961 accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa tgccgttgtc
     7021 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
     7081 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
     7141 aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac
     7201 ggaaagaata ataaagccgg gaattaatgc cgagaaggaa cgcgatactg aagcaggtac
     7261 gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
     7321 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
     7381 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
     7441 cccagccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
     7501 tgccattaaa gacattatcc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
     7561 accattaagt gtttcaatgg ttgaggcatc caggcgaata cctaaggaat aaaaaaacga
     7621 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
     7681 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
     7741 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
     7801 atacccttaa aaacc
//
LOCUS       DAZFFG010000178         7647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFFG010000178 DAZFFG010000000
VERSION     DAZFFG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.179"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..355
                     /gene="ydjO"
                     /locus_tag="ACHJ7J_004594"
     CDS             <1..355
                     /gene="ydjO"
                     /locus_tag="ACHJ7J_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO5002717.1"
                     /translation="VGSIFAAIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGILE
                     DIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHL
                     KSKVTAPIQNGPLPA"
     gene            complement(359..1750)
                     /gene="tcyP"
                     /locus_tag="ACHJ7J_004595"
     CDS             complement(359..1750)
                     /gene="tcyP"
                     /locus_tag="ACHJ7J_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO5002718.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1883..2473)
                     /gene="ydjM"
                     /locus_tag="ACHJ7J_004596"
     CDS             complement(1883..2473)
                     /gene="ydjM"
                     /locus_tag="ACHJ7J_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5002719.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2636..3304)
                     /gene="hxpB"
                     /locus_tag="ACHJ7J_004597"
     CDS             complement(2636..3304)
                     /gene="hxpB"
                     /locus_tag="ACHJ7J_004597"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO5002720.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPKAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3451..3987
                     /gene="yniB"
                     /locus_tag="ACHJ7J_004598"
     CDS             3451..3987
                     /gene="yniB"
                     /locus_tag="ACHJ7J_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO5002721.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4028..4888)
                     /gene="yniA"
                     /locus_tag="ACHJ7J_004599"
     CDS             complement(4028..4888)
                     /gene="yniA"
                     /locus_tag="ACHJ7J_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO5002722.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4994..5284)
                     /gene="ghoS"
                     /locus_tag="ACHJ7J_004600"
     CDS             complement(4994..5284)
                     /gene="ghoS"
                     /locus_tag="ACHJ7J_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5002723.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5385..6314)
                     /gene="pfkB"
                     /locus_tag="ACHJ7J_004601"
     CDS             complement(5385..6314)
                     /gene="pfkB"
                     /locus_tag="ACHJ7J_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO5002724.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6601..7359
                     /gene="ydiY"
                     /locus_tag="ACHJ7J_004602"
     CDS             6601..7359
                     /gene="ydiY"
                     /locus_tag="ACHJ7J_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO5002725.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7412..7519)
                     /gene="yniD"
                     /locus_tag="ACHJ7J_004603"
     CDS             complement(7412..7519)
                     /gene="yniD"
                     /locus_tag="ACHJ7J_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO5002726.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         1958 a         1881 c         1868 g         1940 t
ORIGIN      
        1 tgtcgggagc atttttgctg cgattcaaaa aaatgagagt ctcctaaagt ttccttcgat
       61 aatatcaatg cttaatatca atcttctttt taatccacta aatcttccgg gaatgggaag
      121 tggtatactt gaagatatta tgagtattcc agatagttca cttagaaaac gtctcgggta
      181 tgaggtttta agcttctcgc tacaggcaca ttcattgagc caggaatgta ttgataagtt
      241 ggacattttt ttcgctgacg atctgtttaa atatgagtca gtatgcatcg ccgcgatgga
      301 acatttaaaa agtaaggtaa cggcccctat acaaaacgga ccgttgccag cataagaatt
      361 aatggtgtgc cagttcggcg tcgtcttcgc tatccagaat ggctttatcg gtttgcttca
      421 gccactggct ggtcagcgtg ccagctgtca tcgagccact aacgtttagc gccgtacggc
      481 ccatgtcgat aagcggttca acggagatta acagcgccac cagggttact ggcaggccca
      541 tcgcaggcag cacaatcagt gcggcgaaag ttgcaccacc accgacaccg gcaacgcctg
      601 cggagctaac ggtaacaata ccgaccagcg tcgcaatcca catcgggtcc agcgggttaa
      661 tgccaaccgt aggcgcaacc atcaccgcca gcattgccgg atacaaaccg gcgcagccgt
      721 tctgaccaat ggtcgcaccg aaagaggcgg cgaaactggc gatggattca ggaacgccca
      781 gacgacgcgt ttgtgcttcc acattcagtg ggatagacgc agcactggaa cggctggtaa
      841 aggcaaacgt cagcacaggc cataccttac ggaagtactt cagcggactc acgccattaa
      901 tgcccagcag aatgccatgc actgcaaaca taatcagcag accgaggtag gacgcgacaa
      961 cgaaacttcc cagtttgatg atgtcttgca ggttagaacc tgcaaccact ttggtcatca
     1021 gcgccagaac gccgtaaggg gtcaactgca tgaccaggcg aaccagcttc attacccagc
     1081 tttgcagggt atcgatagcg gttaagacgc gttcaccttt cggcgcatca tccttcagca
     1141 gtttcagcgc agctacaccg aggaatgcgg caaaaattac cacgctgata attgacgtcg
     1201 gattggctcc ggtcagatcg gcaaacgggt ttttcgggat aaaggacaag accagctgcg
     1261 gaacgctcag gtcagagact ttaccaacat agttactttc gatggcgttc agacgtgcag
     1321 tttctgcacc accctgaacc agaccttcag ccgtcaaacc aaacaggttg gtgaccagca
     1381 caccgaccag cgccgcaatc agcgtggtaa acaaaagcgt accgatggtc agaaaactga
     1441 ttttgcctaa ctgagatgcg ttatgcagac gggcaaccgc gctcagaata gaggcgaaga
     1501 ctaacggcat aacgatcatt tgcagcagtt gaacatagcc gttaccaacg atgttaaacc
     1561 actgtacaga atctttaagt acctggctgt cagaaccata aatggtatgc agggcaaggc
     1621 caaaaaccac acccatcacc agacccacca gcactttttt cgccagactc cactgtttat
     1681 ggcgggtctg agccagcgca aacagcagta caacgaacac cacgatgttc gcaattaatg
     1741 gaaagttcat ccccgttctc ctgatcttat tatttctatc actgcgtagc agtcatagtg
     1801 ttggcgcaag attaacagaa ctgtgagctg gctcttatat tcaaatggaa ttgcttattc
     1861 cagaaaggtg atttttgcct ttttagtatt caacctgatg cttaataagc cgatgaaact
     1921 ggatctgcaa ggtattgatc atttgcgatg accaacgaac agcgctgttc tcgggtaatg
     1981 aatggggcat ccataccgac cagacaaata atgccatgca gataaaacgt tccagttggt
     2041 tacccttttg gggaaccagg ataggcaagc ggaaacgcca gcggcatggc cagagcaggg
     2101 gaacaccggc gggtgtcagc atatcggcaa gtatgtggct taaataaccc agcaccattc
     2161 cttgtagcgc atcagccgga atgaaccagc tttcaggaac cttaaggtaa aaggttgcca
     2221 gcagcgcaaa taccgccagc agactgtggg taaaaccacg atgcccaaaa gcgcgggcga
     2281 tcggttttga tatccacttt aatcgctgcc caagaaacga ctttgggtga tcgatgtccg
     2341 gtaacaaaca cgtcaggatt gcggaaggga caatatgcca ccagtcaccc tgtgccagca
     2401 cgggcgtcag ctcggcattt ttggcaaata ccgcacaagc aatagaaaag agaaggtgac
     2461 cttccgccgt catgataaaa ctcactaaac tgtcgatacg tacagtatag gatttttata
     2521 cagtgcacgg aagtggtgac ggtgtaactc tttctcttaa attgacgatt aatccgtcag
     2581 ttgaatgcaa attcagataa agaaccaggg gcagcgtgat cactgcccct ggtcattaac
     2641 cgagaaggtc ttttgcggtg agttctgtca gcgatgagag tttgacgttt gctaatacaa
     2701 aacgtggatc attttgcgct tttggcgcag gaacgacgat ggaacgcatg cgggctgctt
     2761 tagaggcgat catgccattt accgaatcct ccagcgctac gcaggtcagt gggtcaacgc
     2821 ccagttttgc tgcgcagtcg agatatactt gcggatgcgg cttgctgtaa ggcagttttt
     2881 cggccgaggc gagggcatcg aaactgtcgc gtaagtcaaa catggtcaac actttttcca
     2941 gcatatgtag tggtgacgcg gaggccagtc ccaccaataa accttgttct ttgcataacg
     3001 caacggcttc gcgcacgcct ggtaataatg gacgtgtctc ttcaaccagt gaaatggcac
     3061 gggcaataac ccgttctact acttcctgac ggcttggccc attccacggt tgccgggcgt
     3121 accaaagatc gaccaccata tcgatgcgta aacctaaggt atccggcagc tcgttacgac
     3181 gggagatatc cacccccagg cttgccatca catccagttc ggctcgatcc cataaaggtt
     3241 ctgagtcgat aagtaatcca tccatatcaa aaattgcagc aagaatctga cgcggggttg
     3301 acattacaac ctctccttta gagcattatg tgtaaggtat taagccgtgc ttatgattaa
     3361 gcgcctatca tatcgccttt aaagaatggg cgaaaacaga tatctacagg taaactaagt
     3421 gaaacgacgc gtcttaatca aggagaactc atgacgtatc aacaagctgg acgcattgct
     3481 gttttgaaaa ggattttggg ttgggtgatt tttatacctg cattgatctc tacattgatt
     3541 tctttgctga aatttatgaa tacccggcag gaaaaccagg aaggcattaa tgcggtcatg
     3601 ctcgacttta ctcatgtcat gatcgatatg atgcaggcga atacgccttt tttgaatcta
     3661 ttctggtata actccccgac gcccaatttt aatggtggcg tgaacgttat gttctgggtg
     3721 attttcatcc tgatttttgt cggactggcg ttgcaggatt ccggtgcccg gatgagtcgc
     3781 caggcgcgct tcttgcggga aggtgttgaa gatcaactca ttctggaaaa agccaaaggg
     3841 gaagaggggc taacgcgtga acaaattgag tcccgtattg ttgttccgca ccatactatt
     3901 ttcctgcagt ttttctcact gtatatcctg ccggttattt gtattgctgc aggttatgtg
     3961 ttcttttctc tgcttgggtt tatttaatca aaaggccgca cggagcggcc attatcttca
     4021 acccttatca tgctgctaat aatctatcca gtgactgctg agcaataacc aaatgctgac
     4081 cgccaaataa ccttgcacga tttaacagcg tgtagagttg gtaaaccggt tgacgttcaa
     4141 ggaaatcggc aggtagcggg gatactgact gatagccgtc atagatttgt ggcggctgtt
     4201 cagtatgcag cggtaacatc gccaggtcgc actctcggtc accccagtag caggccgggt
     4261 cgaaaatgta cgggccatcc ggacccagcg cacaattgcc ggaccataaa tcgccgtgca
     4321 acagagaagg ctgcggttga tgcgaggcca gacgctgctg aatatgctcg acgatggcgt
     4381 cgatattgcc gaaagcgatc cctttttctg ctgccagttc caactgccag ccaatccgtt
     4441 gttcagcaaa aaacgttgac cagcgacgtt gccaggtgtt gggctgtgga gttgtggaga
     4501 gcgcgttatc gaaatcgagt ccaaattgtg gttggtcgct ccattgatgt aaacgcgcaa
     4561 tttgctgacc aagaataaat gcgctatgcg catccagcgg acgaggtggg agataatcca
     4621 tcaccagaaa actgtagtca cggtcagcgc caactgccca aaccttaggc acggtgacgg
     4681 ttttactacg cgacagtaac tccagttggt cggcttcggc ggtaaaaccg ggaagcagtt
     4741 ccctttcatc acatttgacg aaaaagtcat gtcctgcata gcgcaaatgc catgcggcat
     4801 ggacttctcc gccaggcagt tcattacgca gttcgatttc gccttcacct aactgctcgc
     4861 tcaaaagacg actgattgcc tgccacatga tgtctctccc atgttgtctg ccagatcata
     4921 aggttagcgc aagaatgcgg tgtaaaaaca cgagctaacg cacaccacag actgattatt
     4981 gcccaaaaac catttatttt ctgttgctat tccattcctc ccaggtcgtg atggtgattt
     5041 caggatcttt gccggttgca ctttgggtaa gccccgaaac cagttctcta atttcttctt
     5101 cactttgggt actgataagt ccaaaagtgt tagtccccag ctcatggata tttccctcat
     5161 catccgtcat ggtgagcaaa aaaccgtcgc gagtcaggta attattcaac tcgttaattt
     5221 cagtcaatgt atcctcatgc aacatgacgg ttatgacata acggacaaga tcgccactgg
     5281 ccatagttca cccctttgtt atcacatgac ttttgagcat agtcggagaa acgcgttgcc
     5341 gacaggttgg tgatgattcc cccaatgctg ggggaatgtt tttgttagcg ggaaaggtaa
     5401 gcgtaaattt tttgcgtatc gtcatgggag cacagacgtg ttccctgatt gagtgtggct
     5461 gcactccccg cagctacgcc aaaacgaacc atctcttcaa gagaggcatt ttctgccagt
     5521 ttcagtgtca tcgcgccgac catgctgtca ccagcgccaa cggtactctg gcttttcacc
     5581 ggtggtggca ccacctgaat gcagttttca ctatcaacac ccagcgctcc ttgtggaccc
     5641 agggaaacga caacccgttt ggccttgccg ctattaacga tttcctgcgc ggctttgcgg
     5701 acatcgtctg gctgggtgag ttcgcgattc accagcgcac tgagttcttt ttggttaggc
     5761 ttaaccaact cgatgttacc aatcgccagt gctgcactta acgcttcgcc agaactgtcg
     5821 atgatgcagc ggatcccttg gttttgcgca gcggaaatca gttgggttaa tttttccagc
     5881 ttcacacctg gcggcaggct tccgcttatg accaggatgg ccccggattc aatttccaga
     5941 acttgctctt caagctggcg aaactcatct tcatttaatg ccgcgcctgg cataacaaaa
     6001 cgatactgct caccgcttgc ttccacatgt acgtgtaaat tctgccgggt ccagtctttg
     6061 gcttctacag tagcgacggg gacattttca tccgccaaca gtgaaaccag gtgttcgccg
     6121 gtcgcgccac ccgccgggaa gatcgctgtg gcactgcctc caagatgggc aatggcgcgg
     6181 gcgacgttga tgccgccgcc cccgggttcg aacaccggtg cggtacagcg cagttttcct
     6241 tcgggataaa tttgcggggt aattgttgcg ctatcgagag agggcgcaag tgtcaacgta
     6301 tagatacgta ccatcatttc ctcctatagg ctgatttcag tctggcaccg aatcagcgga
     6361 aagtgaagaa attaacaata tgatttattg gagctttatt taaaattttg cagataaata
     6421 tatataaata aaaatctgtt ttcgccacat gcttattcaa aaaatgaaat aagaatacat
     6481 tgctaagtta ttcgcgcctt tttataattc gttacctttc cagggacgct aatctttgat
     6541 ccttaagaaa gtttccggga ccgcgatggt ctcttttttt gttgtacgga ctcttgataa
     6601 atgaagcttt tgaagacagt tcctgccatc gttatgttgg cggggggcat gtttgcgtca
     6661 ctgaatgcag ctgccgatga ttccgttttt actgtcatgg acgatcctgc cagcgctaaa
     6721 aaacctttcg aaggtaatct gaacgcaggg tatcttgcac aatcaggcaa tacgaaaagc
     6781 tcctcactta cggctgacac caccatgacc tggtatggac aaaccactgc ctggtcgctg
     6841 tgggggaacg ccagtaatac ttcttctaat gatgagcgtt cttcagaaaa atatgcggcg
     6901 ggcgggcgta gccgttttaa tttaactgat tatgactatt tatttggtca ggcaagctgg
     6961 ctgacagacc gttataacgg ctatcgagag cgcgatgtgt tgaccgcggg ttatggtcgt
     7021 cagtttttga atggcccggt acacagtttc cgctttgaat tcggtccagg tgtgcgttac
     7081 gacaaatata ccgacaatgc cagtgaaact cagccgctgg gctatgcctc cggtgcttat
     7141 gcatggcagt tgactgacaa cgctaaattt acgcagggtg tttcggtctt tggcgcggaa
     7201 gacacaacgc tgaactccga aagcgcatta aatgttgcta ttaatgaaca ttttggactg
     7261 aaggtggctt acaacgtcac atggaactct gaacctccag agtctgcacc ggaacatacc
     7321 gaccgtcgga caaccttatc gctgggctac agcatgtaaa attatctgcc ggaccgtgtt
     7381 atgcggtctg gctgtcttcg tttgtcactg ctcagcccca aatcattgct gcccaacgta
     7441 gcaacaacat agcgccacag attgccagta gcaccaccac catcttccag tgttttttat
     7501 caaagcgttt agtcggcata ccagtgtcct gcatatctct tgtgcgtgat aacggagtgt
     7561 aacaaaaggg acaggcatca agggattttt tgttattaca gccgtcgggt ggcgttgtgc
     7621 acatggtcac tgtttctgcg ttttatg
//
LOCUS       DAZFFG010000179         7604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFFG010000179 DAZFFG010000000
VERSION     DAZFFG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.180"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..354)
                     /locus_tag="ACHJ7J_004604"
     CDS             complement(<1..354)
                     /locus_tag="ACHJ7J_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO5002727.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFH"
     gene            complement(611..1246)
                     /gene="msrQ"
                     /locus_tag="ACHJ7J_004605"
     CDS             complement(611..1246)
                     /gene="msrQ"
                     /locus_tag="ACHJ7J_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO5002728.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     TPIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1247..2251)
                     /gene="msrP"
                     /locus_tag="ACHJ7J_004606"
     CDS             complement(1247..2251)
                     /gene="msrP"
                     /locus_tag="ACHJ7J_004606"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO5002729.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLDFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2360..2773)
                     /gene="hiuH"
                     /locus_tag="ACHJ7J_004607"
     CDS             complement(2360..2773)
                     /gene="hiuH"
                     /locus_tag="ACHJ7J_004607"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO5002730.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2906..3577
                     /gene="hprR"
                     /locus_tag="ACHJ7J_004608"
     CDS             2906..3577
                     /gene="hprR"
                     /locus_tag="ACHJ7J_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO5002731.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3577..4935
                     /gene="hprS"
                     /locus_tag="ACHJ7J_004609"
     CDS             3577..4935
                     /gene="hprS"
                     /locus_tag="ACHJ7J_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO5002732.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     NVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(5033..5884)
                     /gene="hchA"
                     /locus_tag="ACHJ7J_004610"
     CDS             complement(5033..5884)
                     /gene="hchA"
                     /locus_tag="ACHJ7J_004610"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO5002733.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLVAYAG"
     gene            complement(6477..7592)
                     /locus_tag="ACHJ7J_004611"
     CDS             complement(6477..7592)
                     /locus_tag="ACHJ7J_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5002734.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNYD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADREGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
BASE COUNT         2085 a         1616 c         1788 g         2115 t
ORIGIN      
        1 atggaattca acaatgccgt cctcaatgcc aatcatctcg atatctgttg cataaccttt
       61 gtgataataa tctttaattt cagcaaatgt tttagtttta tctgcatccg ctttcttctg
      121 aaagacgggg gcaagtttgc cactttgcag taaaggataa acggattgcc agactccatc
      181 ccagtcactg agcgttcggt tttgtacatt ggcatcatca aaaacaccgt tagcagcttt
      241 ttgttcgacc tctgttaagg gtttgccgtg tgagtgatga ccatgcgaaa aggcaggagc
      301 gctaacaata aagacaccta aagcaacagc cagtttgtga agacgaatcg ccaaaacagt
      361 ttcctccaga gtcattgtat atgatgtgta atgttatatt gtaacatatg acaaaatgca
      421 acaactctta ggagatgaga gcatagtttt ctcaggtgtg cttatctatc ttattgaaat
      481 aaaatgaatt atgttgataa tttgaggttg atgacaaacg aaacataacc tgatgtgatc
      541 tgcgtaggcc ggatagggcg ctcgcgccac atccggcagg gataacgcgt actttgtcgg
      601 caatatgaag ttatacagat aatttgttgt gaacctgctt gcgtaaccgg ttaaaaaggg
      661 aacgcaactt cttataccgt aaggctaaaa gcagtacagc cagcccagcg tagatgagcg
      721 gctgcggtga aataatcttc actgaccaca gataatgtat tggggtcagg atcgcgacaa
      781 gatagacgaa attatgcaac tgttgccagt gcttgccgag ttttcgctgc atcgcctggg
      841 ttgaagtaaa cgctaaagca agcaaaataa cccagctgat aattcctaac gttaaataag
      901 gccgggtaat taactccttg cctaataacg ccagattatt cacgcccagc tccagcaatg
      961 cgtaactggt taaatgcagt gtcgcccagg caaagcacca taagcctaac aggcggcgag
     1021 tgcgtatcaa taacggctgt tttgcgtagc gtgccagagg tgtgattaac aaggtcgcca
     1081 gcaaaaattt cagtgcagtg cgaccggtaa aatgctgaat atctttcacc ggatcggcac
     1141 ccagtccacc gtgattgatc gcccagacca gccagagaaa cggcaacaat ccggcaagat
     1201 gcaggcaaac tttcagccat gtcacctgtt ttgctgtcag acgcatttag aaattctccc
     1261 gcaaatccag gccacgatac agcgatgcca cctggtcggc gtaaccatta aacagtagcg
     1321 ttggctggcg ctgtacatcg aggatgccgc ctgaaccaat aaatcgttcg gtagcctgtg
     1381 accagcgcgg gtgatcaaca tgcggattaa cgttggcgta aaaaccgtat tcgtcaggcg
     1441 ctgccagatt ccaggtggtt ggcggacgct cgcgggtcag cttaatactg acgatcgatt
     1501 taatcccttt aaagccatat ttccacggca caatcagtcg caccggcgcg ccattttgtg
     1561 gcggtaacgc cttgccataa acacctacgg tcatcagtgt gagcggatgc attgcttcgt
     1621 cgagacgcaa tccttcgaca taaggatatt tcagcccgcc gccgataaag cggtcctgct
     1681 ggcctggcat ctgttccggt gcataaattg tttcgaaagc gacatacttc gcattgctgg
     1741 tgggttcggc aagcgccagc aatttgtgca gtggaaaacc aatccacggc accaccatcg
     1801 accatgcttc tacgcagcgc atacgataaa tacgctcttc cagcgggaag cgacgggtta
     1861 agtcatcatg atcgagggtc aatggttttg ccacttcgcc gctgattttc agtgtccatg
     1921 gatcggtttt caggctacca gcattagcgg cgggatcggc tttatccagc ccgaattcat
     1981 agaagttgtt ataaccggag actttatctg ccggcgtcag tggcaggtta ttttgccagg
     2041 cggcaggctt gctgaagtcc agcggttttc cggcgggggc gggcggacga tcgttccctt
     2101 taaaccagct aagcagatcg gcatgtgcag cgtggggcaa agaaagtgca gctgcgctga
     2161 tgcccagtgc ttttaacacc tgccgacgct tcataaagaa taccgactcg gccgtaacat
     2221 ctgattcttt taaaaattga ttctttttca taagccatca cacttttttg tcgttagcca
     2281 gcaagagccg tttactgtta atctgtcagg gagtgtcctc cctaacagta gcagagatct
     2341 gcttttacat atttatgcat taactgccac gataggttga atacccatat tggctaagta
     2401 ataaaggcac atgataatgc tcgttcactt tattaatatg aaattcaacg gggatctcag
     2461 ggaagaaact ttcaagattt tgtttcttga aatagtcccc ggttttaaat acgacacggt
     2521 aatcgcccgt agttgcagtt tgctcgggcc acagtgcctt aattcgtcca tccttatctg
     2581 ttttggcggt attaagttgt aaccagccgt tgtccgcttt cttttcaaga gtgactgtca
     2641 cgtcggcagc gggttttcct gtttgctggt tcaaaatgtg cacgctaaga atgttttgtt
     2701 gtgcagcata gaccaaagaa ggtaatgaaa atgcagccgt tactacggag agtactaaat
     2761 aacgctttaa catgtttata tccttgtcat gtgaatgagt tttgaggata gcgaaaaagc
     2821 gtccagggta accttacagc agcattacaa ttttgtaatg aaccaggctg tttctataac
     2881 atatgattta tggcatatta ttttcatgaa gattttactt attgaagata atcaaaggac
     2941 ccaggagtgg gtaacgcagg ggctttccga agcgggttat gtcattgatg ccgtttctga
     3001 tggcagagat gggctttatc ttgcgctgaa ggatgattat gcattgatca ttctggatat
     3061 tatgcttccg ggtatggatg gctggcagat cttacaaacg ttaagaacgg caaagcaaac
     3121 ccctgttatt tgccttactg caagggattc tgtcgatgac agagtcagag ggctggacag
     3181 tggagcaaat gattatctgg taaaaccttt ttcattttct gagttgctgg caagggttcg
     3241 ggcacaatta aggcaacatc acgctttgaa ttcaacatta gaaatcagcg gcttaagaat
     3301 ggactctgtt agtcaaagtg tgagcaggga caatatcagt attacactga cgcgcaagga
     3361 gtttcagtta ctttggctac tggcctccag agctggcgaa attataccca gaacggttat
     3421 tgcgagtgaa atttggggaa tcaactttga tagtgatacc aatacggtgg atgtcgccat
     3481 tcgcaggctc cgcgcaaaag ttgatgatcc ttttcctgaa aagctaattg ccacaatccg
     3541 tgggatggga tattcgttcg tagcggtaaa aaaataatga aaagattgtc tataaccgtc
     3601 cgtttaacct tgctttttat attgctactg tctgttgctg gtgctggaat tgtctggact
     3661 ctctataatg gcctggcaag tgagttgaaa tggcgcgatg atacaacact cattaaccgg
     3721 acagcacaga tcaagcagtt gttaattgat ggggtaaatc cagatacgtt acctgtgtac
     3781 tttaaccgga tgatggatgt tagtcaggat atcttgatca ttcatggtga tagcatcaat
     3841 aaaattgtta accggacaaa tgtcagtgat ggcatgttaa ataacatacc tgctagtgag
     3901 acaatcagcg cagcgggcat ttacagaagc attattaatg atacagagat agatgcttta
     3961 cgaattaata ttgatgaagt ttcgccatca ttaacggtta ctgtggctaa attggcttca
     4021 gccagacata acatgcttga acagtataaa atcaatagca ttataatttg cattgtcgcc
     4081 attatactgt gctcagtatt aagtccgctg ttaatccgaa cggggttacg agagatcaaa
     4141 aagttgagtg gtgtaacgga agcgctgaat tataacgata gccgggagcc tgttgaggtt
     4201 agcgcattac cgagagaact aaaacctctt gggcaggcgt tgaataaaat gcatcatgct
     4261 ttagtcaaag attttgagcg tctaagtcag tttgctgacg atctcgctca tgaacttaga
     4321 acgccaatta atgcattact gggtcagaat caggttacgc tcagtcaaac cagaagtatc
     4381 gctgaatatc aaaaaacaat tgccggaaac attgaagagc tggaaaatat ttcgcggtta
     4441 acagagaaca tactgtttct tgccagggca gataaaaaca atgttttggt gaaactggac
     4501 tcgctttctc tcaataagga agtcgaaaat ttgttggact atcttgaata tctttcagac
     4561 gagaaagaga tttgctttaa tgtcgagtgc aatcagcaaa tctttgcgga taaaatttta
     4621 ctgcaacgaa tgttatcgaa tcttattgtt aacgctattc gatattcgcc agaaaaatcg
     4681 cgtattcata taaccagttt tcttgatacc aacagctatc ttaatattga tatcgccagc
     4741 cctggaacga aaattaatga gcctgaaaaa ctcttccgta gattttggcg gggagataat
     4801 tcgcgtcatt ccgtaggtca gggactaggc ctttctttag tcaaagcgat tgccgaatta
     4861 catgggggaa gtgctacgta tcactatctc aataagcata atgtgttccg gattacgtta
     4921 ccgcaaagaa attaacatag ggcttcagta tgcccgtgaa aaaacgcagc cacgtcgtga
     4981 gtactaacgc ggctgcgatt gattatgcgc ttacattcaa acgtaacagg gattaacccg
     5041 cgtaagctac cagcatttcc tgcgccgcca gtttacccaa cgcattcgct gcaaaaggac
     5101 tgtcgccggt gagaagttta cggtccttat gtactcgccc ggtgatgtcg tcattaatga
     5161 tgttcatgcc cattttcttc agttcttcgc cgaagtacca ggtgagatga cccggcatat
     5221 agccaatctc tggcgtttgt ttgtctgcgg cgtctgggaa tgcgcaaatg gaataaccat
     5281 tcagtgggtt atcgccgtgg cgaagcgcca gaaaagccgc cgggccgtgg caaagggaga
     5341 taacaaaacg gtcatttttg attgcccact gtaaagcggc agctacgtcc tggctttcag
     5401 gtaaaccaat aagtgcgcca tgaccaccag gaacaaagat tgctgcatat tcgctatcag
     5461 cgttgaggct ggcgacaaca tccgcgagtt tcttcggatt gcggaacaac gatttatgct
     5521 gctcaaagaa tggcattact ttttcatctt tgtgcggcat agcccagtat tcaaacttgg
     5581 tcatcagacc ggaaatggtc gccacttcga attcgaaacc tgcagcatgg agatgataca
     5641 acggcagcaa cgtttcaatc ggatggttac cggtcgagaa cagctttccg ttatcggtcg
     5701 gcaaataacg ttcgtccgcg gcgatcacca gaattttatg tttaccgcga tacggttttg
     5761 gatagtccac gccatcaaga tcagagacag gactggtata ttggctaagc gaatattctg
     5821 aagggaagaa tgcattatct tcagcaatat cgacctgcgg atttttactt gtttgaacag
     5881 tcatagtgta ttccttattg ttgcttagtt agggtagtca ctatatttgc gactgagctg
     5941 gtcgctattt ttaaccagac tggaaaattg agtcaatata tcgaaagcgg tagtcggcgg
     6001 gtgtaagtat gtaatgatga tgctgcactg gtgttgaata ttattcacgc caaattttcc
     6061 ttattgagca acgtcgtcgc aagtggcctc aatgatttta gaccattatc agaggtacgg
     6121 ggcggcgggg agagatttag tgatgtggaa tattgtggtg ctgatgaata tttatatccc
     6181 ggattggtgg agaaaaagct ttttccatgc actttttttg caaatatttc tatatgggag
     6241 gcatgtttgt tgcggattaa ttcataaaga aatgtatttg gagtgttggc tggaagagta
     6301 gctggtagaa gatattgtgc caggaggggc agggtaaggt ggtgtatcga tatgctttgg
     6361 caattgcgtg tgccgcagat ggagatgaaa tgtcaataaa aaagcctgag ttcgatgagg
     6421 gacgaactca ggctagtgtt cagttttaca gtaacacgcc gcggcgcgtt ggctgattag
     6481 aactggtaga cgataccaac tgcaaactgg tcatcagctg ctgcgccagt aatggcggcg
     6541 tcatctttat ccagcatgtt gaatttgtat gcagtataaa cgttcatgtt cttattaaag
     6601 tagtaccagg taccaacttc aatgtagttc acacggtcag cgtcaacata tctatgtcca
     6661 ggtacccctt tgatatcttt acctttagag tacacataac ctaaggacgg acgcagacca
     6721 aagtcgaatt gatactgaac aaccgcttca aagttctggg tttcattgat aagttgatca
     6781 cgcattattg tattgccttc tcgatcggca acacctgtat caatagaacc cggagtcata
     6841 ttacgagttt cgccgtacat tacagctgcg tagacattgt tcgcgtcata ctttgcagcc
     6901 aatgaccaca gttcagcatt tgaaccgttt ccatcggcaa cttgatcgtt ggtgcgatca
     6961 gatttgctat atgcaccaac gataccaaaa ccattgatgt tatagtctac agacaacgca
     7021 tagccgtcac cattttgttt cttagtgcta cggttgctgt ttttgccttg gtactgaatc
     7081 gcaaaatcaa gaccatcaac caggccaaag aaatcatagt tacggtaggt tgcaacaccg
     7141 ttggtacgac cattcatgaa gttgtcagcg gaagcccagg aatcgccacc ccattcaact
     7201 aacatatcgg tataagctgc ggcatcataa gcaacaccgt agttacggcc atagtcaaat
     7261 gatcctaact cgttatattt caaaccggcg aaggttaaac gggttttttc ctggttttca
     7321 ccttcgggct tgctggcgtc gaggttgtat tcaaactgac caaaaccagt cagttcgctg
     7381 gtgatttggg tttcaccttt aacaccgaaa cgagcatagg aggtatcttc gttttcgctg
     7441 ttattatcat cagtatttga ccagatgcgt tcgccaacca tcttgccgta gaaatcaact
     7501 ttattgccat ctttattata aatttcagcc gcatttgctg cgccagcaac taataacgcc
     7561 gggaccagca ttgccagaac ttttcttttc attatgtatt ccct
//
LOCUS       DAZFFG010000180         7519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFFG010000180 DAZFFG010000000
VERSION     DAZFFG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.181"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..674)
                     /locus_tag="ACHJ7J_004612"
     CDS             complement(<1..674)
                     /locus_tag="ACHJ7J_004612"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO5002735.1"
                     /translation="MDEKFEQKKSGLTVALSGTVGSAINNAATMAREAKETSDSRLKA
                     LQATKTALSGVQAGQAAAMASATGDPNAMGVSLSLTTQKSKSQQHSESDTVSGSTLNA
                     GNNLSVVATGKNRSDNRGDIVIAGSQLKAGGNTSLDAANDILLSGAANTQKTTGRNSS
                     SSGGVGVSIGAGKGAGISVFASVNAAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVL
                     NGAQVNG"
     gene            complement(822..1058)
                     /locus_tag="ACHJ7J_004613"
     CDS             complement(822..1058)
                     /locus_tag="ACHJ7J_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002736.1"
                     /translation="MKSDFSVDFFSDSRYEELTAEISYKGQILCQLNKDKGANAVEVE
                     FFTDSRLLPESIEMKFSLEEFICILNEAKLELLA"
     gene            complement(1246..1764)
                     /locus_tag="ACHJ7J_004614"
     CDS             complement(1246..1764)
                     /locus_tag="ACHJ7J_004614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002737.1"
                     /translation="MDLINPSQRRMRRSERITGKYQQAIQAATAAVQGLAGGNLSAAL
                     AGGAAPYIAEIIKQTTPDGAGRVAAHAVVNATLSLAQGKNALAGAAGAATGEVVGMIA
                     TQMYGKPVNELSETEKQTVSTKFNPVILMINSKRKILSLRLINQLIIRDYPQQRVHGK
                     SMLESLEGYLIL"
     gene            complement(1755..2111)
                     /locus_tag="ACHJ7J_004615"
     CDS             complement(1755..2111)
                     /locus_tag="ACHJ7J_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFO5002738.1"
                     /translation="MITELSEREYKELADAAEGQTLDAILCYSVPELHPAEFSFADRR
                     EIFFWALTRLLKEGRIKLAKHGKFLEGSVDEQVERFRQAFPKTEEEMEDGIWFFDESC
                     PGGAVWVLEDGSLEWT"
     gene            complement(2825..7519)
                     /locus_tag="ACHJ7J_004616"
                     /pseudo
     CDS             complement(2825..7519)
                     /locus_tag="ACHJ7J_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
BASE COUNT         1492 a         2216 c         1780 g         2031 t
ORIGIN      
        1 ccgttgacct gagcaccgtt cagtaccgta tcccgaccgc tgttgatggt gacggtttta
       61 ccgctgtctg ttgtggtttc agtccactct gtaccgttac ctttctcgct gccttttgcc
      121 gcattaacgc tggcaaagac gctgataccg gcacctttac ctgcaccgat actgacaccc
      181 acgccaccgc tactgctgct gttcctgccc gttgtttttt gtgtgtttgc agcgccactc
      241 aacagaatat cattcgcggc atccaggctt gtgttaccac cggccttaag ctggcttcct
      301 gcaatcacaa tatctccgcg gttatcgctc ctgtttttac cggttgcgac aacagacaga
      361 ttattcccgg cattcaacgt actgccggat actgtgtcac tttcagaatg ttgttgtgat
      421 ttcgatttct gggtggtgag cgacaggctg actcccatcg cattcgggtc accggttgcg
      481 gaggccattg ccgcagcctg tccggcctgt acaccagaca gcgctgtctt tgtggcctgc
      541 agggctttca gacggctgtc gctggtttct ttcgcctccc gggccatggt ggcagcatta
      601 ttgatggcac tgcccaccgt gccggaaagg gcaaccgtca gcccgctttt cttctgctca
      661 aatttttcgt ccacagtacg acggtcatgc cccctggtca atgtacctta cccgctttgc
      721 tgctttatga gcaacattac tataagcttt agccgccaag gtttctgccg cagttttacc
      781 tacaccgata gagctatgct gacacagtag tcaggttctg ttcaggcaag tagctctaat
      841 tttgcttcgt tcaagataca tataaattcc tctaatgaga atttcatttc tattgattct
      901 ggtagtaatc gactatctgt aaaaaactca acttcaacag cgttagcacc tttatcttta
      961 tttagctgac agagtatctg tcccttataa gatatctctg cagtcagttc ttcgtaccga
     1021 gagtcactaa aaaaatctac actaaaatca cttttcatac attttatctc tttggatcca
     1081 ggaaacctga aaatgagcca tcagagttat agcgaacacc cctaccatca ggtaaacggt
     1141 attccacacc acctcttgaa atatctgttc taacagtttc attattgttc aatacctcat
     1201 ttacgaagtt accagccact tcatttttct gaactgtatt ccctttcaaa ggatcaaata
     1261 cccctccagg ctttccagca tgcttttccc atgcacgcgc tgctgcggat aatccctgat
     1321 tattaattgg ttgattagcc gaagagataa gatcttcctt tttgaattta tcatcaaaat
     1381 tactggattg aattttgtcg aaacagtctg cttctctgtc tcgctcagtt cattcaccgg
     1441 cttaccgtac atctgtgtgg ctatcattcc caccacctct ccggtggctg cacctgcggc
     1501 accggcaagg gcattcttac cctgtgccag agacagggtg gcattcacta ctgcatgagc
     1561 tgccacacgc cctgctccgt ccggggtggt ctgtttgata atttcagcaa tatacggcgc
     1621 agcaccacct gccagggctg cactcagatt accgccggcc agtccctgta ccgctgccgt
     1681 tgcggcctgt atcgcctgct ggtatttccc tgttatacgc tcgctgcgcc gcatcctgcg
     1741 ctgtgaaggg tttatcaggt ccattcgaga gagccatctt caagcaccca aacagccccc
     1801 cccggacaac tctcatcaaa aaaccagata ccgtcttcca tctcttcttc tgttttcgga
     1861 aatgcttgtc gaaaacgctc aacctgctcg tcaacacttc cttccagaaa cttaccgtgc
     1921 ttggcgagtt tgatacgccc ttctttcaga agacgggtta atgcccaaaa aaagatttct
     1981 cttctatctg caaaactaaa ctctgcaggg tgaagctcag gaactgaata acacaaaata
     2041 gcatccagag tctgtccctc tgcagcgtct gccaactcct tgtattctct ttcacttaac
     2101 tcagtaatca tttgaactta atctccacac gtgaatattt attccccagt tcagtaagct
     2161 tttggttatt acgaatatct atagtccaac gagcgcctgt ctgaccttct gatgtagata
     2221 tgtttctaag tgttattacc gaaccatctg atagtttaga cgtgtagatt cccgatttta
     2281 cctctttcag cggtgtatcg cctgccagct gttgtgcgta attatggatc tgtttatccg
     2341 tcagattctg actttcaaac actttcgtcg taccataacg gttacttacc gaactctgcc
     2401 tgatagtctg cccatccacc gttaactctc cggccgggtg acttttatca tatattttac
     2461 tcagttcatc aaatcgttgt tgctgaacct cctgatgacg ggctttttct tcattatccg
     2521 gtccccatcc ccccggtgtt ccagcacctg caccactaag ctcagcttta tccttatccg
     2581 ttgcgctcgc tacgtctttg cccaaattcg gctgtttgtt ttcgtctgta ttgtcgccat
     2641 tcagcacatg ccccggtccc cgggtaccat cgcgactcgg atcacgtaca gcacattgtt
     2701 cccgtgagcc ttcacaacca ccaccggtat cccagctatt atcattctcg gtatccttac
     2761 tggcattacc tgtagcacca gctattgctg ctgacgtatt agctgctgtt tcagtggcta
     2821 tcacgttcag catattgttt tcagccgagt tcctgcctgc ctgtgccccc gtaccggcag
     2881 acgccgtact gttgcctgca agcccgcccg caatacctga ggcaatagtg gccagcatgc
     2941 tgatttgctg acgttcctgt tcactcaggc tatcggtacg accaccgtag tagttatcaa
     3001 tgatataccg ggctgcaagt tcaccactga atgcccccgc agcaccggaa gccgcattgt
     3061 ttccacccag ttgtgctgca agtgctcccc agactgcgtg agccatcagg tttgcttccc
     3121 ggttatcgcc tgtagcctgt tttataagct gtgccactgc cggattcaga ccgccagcca
     3181 gtacctgccc cggattccct gcattcagtc cgcccagcac gcccgtgatg gcctgtacca
     3241 ccatctgcgg cgtactgccc gtaccgtagc ctttcatgac ctctctgtat tccggggtct
     3301 cccgcaactg tgcatcgctc atgccaggat gctctttcct ggctgctttc atcgcttcag
     3361 tttcgccata cgtcatgacg atgttactca tctgaccgct aatctggctg ataacctgcg
     3421 cttcctgcag acgcttctgc tctttctccc tgtcaaatat cggtgcaatg ctgccgttgg
     3481 cgttttccgg gtcccggctg agcccggcaa tatcctgctt ctgactctcc ttattacgga
     3541 taatgatatt tccgccgctg atggcggatg acgtggtacc ttcagcatgt ccactgctgc
     3601 cggacatacc ggtaagtgcg ttcgccgcac cggctattgc atttgaggcc accatctggc
     3661 tggctgacat ccctgagcca cccgataaac tgattcctgt atggctggct ttatagtctg
     3721 cctggttatg gatatccgac cagcccagcg tgccggtact gaggctgttt ttgtcatccg
     3781 ttgccgtgga cgcaattacc gcaccattga gctgagtgtg attccctaca gtgacatcaa
     3841 acccgtcttt tccggcgtag ataccactct gttcccggac agaatcgtaa ttgcttttga
     3901 ttttgtcctg actgatgctg gcatagccgc taccggacat tgagccaaac gtgaagcttc
     3961 cacctgcggc cacgctgttt tgtctgctgt cgtaacggtc actgtcctga aggcccgaaa
     4021 tggtcaggtt gttgccgaca tctgctgtga ctttctcgcc actgacctga gcccccgaca
     4081 gggtggtatc atgtccgctt gtgagggaga catttttacc cgcatccacc atggtttcgg
     4141 tccaggtggt accgttgcct ttctccctgc cctctgaacc attgataccg gcgaagatac
     4201 tgaggcctcc gccattggtt ccaccaccaa agctgacgcc cacgccgcca cctttgctgc
     4261 tgttcttccc ggtggtctgt cgggtgtcag cggcggcagc cagcagaata tcgttctgtg
     4321 cattcaggct ggtgtcattg ccggacttaa tctggctccc ggtaatgaga atatcgccgc
     4381 tgttgttatg gtccttattt ttccccgtgg cagtgacgga gacattgtta ccggcgctga
     4441 gggttgagcc ggatacgata tcgtgctgat atttcgtctc agacttcgac tgctgatggt
     4501 tgagcgaaat gctcacgccg ataccattat tcgggtcagc actctgttgc tgcagtccgt
     4561 ggttaacact ggcctgtaca ccggacagaa ccgcctgtgt tcctttcagt gccgccagac
     4621 ggctgtcgct ggtttctttc gcctcccggg ccatggtggc agcattattg atggcactgc
     4681 ccaccgtgcc ggaaagggca accgtcagcc cgcttttctt ctgctcaaat ttttcgtcca
     4741 cagtacgacg gtcatgcccc gggtcaacca ccacactgtc accggtaatg ctgatatcac
     4801 ggttcgcaat cacatccgaa ccgctgatat gagcctgttt gcccgcggta atactgacat
     4861 taccggcagt ggagccgatg gtgctggcac tctgactctg cgttgtcccg gcctcgcggc
     4921 ggtcgtgcgt tgtcttactg ctgccagtgg tgaagccagt accgccggta cccgtcagac
     4981 cggatgtctt cgtttcctta aagcgccagg acgtatctgt actggtggca gcaagaacat
     5041 caacatggtt acccgccgcc agtgacacat cccggtcagc caccacatcc gacccctcta
     5101 ccgtcaggtt attaccggcg ttaacggtca cgcggtttcc cgacagcagg gagcctttct
     5161 cccgggtgac gctgtcttcc gtgatggtcc gggtggtctg atggctgagg agacggttac
     5221 gggtttcccc tgattcactg tagtgataat cactttccgt ggcagtaccc agtacgatgt
     5281 catgaccggc attcacactg atatcgccct ttgccgttac agcagtcgca actgaggtga
     5341 tatcccgtcc ggcagtaacc gtgatgtcac cgccactggt catgtctgtt ccctgctgcc
     5401 ggaccgaatc atgaaccgtc ttcatgctga aagtgctgtc gtggtcacct gtgcctgtgg
     5461 actccgctgc catgttcaca tcccgtccgg cgcggatagc catactgttt tcagccgtaa
     5521 taccggcagc ctggctgttc acatcacgac cggcggaaag catcagactg ttcccggcag
     5581 tgaccgtggg ggccacggca tgagtttcat accacccgga cccgccatag cgacgacgtt
     5641 cgccggcctg tatcacgttc atgttcaggt cattgccggc ggccagtaac acgctgtcac
     5701 cggcagaaac ggcggcaccg gtgatactga tatctccggc tgcgcccata aacagtgagt
     5761 ccgttgccct gacagcagcc agcggaccgg tatccgttcc gctgaaatgg ttatatccgt
     5821 tgttcatcgc ccactgttct gtacgggtga ggttactgat gtttccggtg gcgctttcca
     5881 gggatacggt tttaccgctg atgactgagc tgatattgcc gatatccccg atggccttca
     5941 ggttcagtgc accaccggcg tttatcagtc cggcattcag gttgtcgata ctacctgtgc
     6001 tgtcgagcag gaggtcattc tgtgcgttga tgctgctgcc actgttggtg atattgccac
     6061 ccgcaatctg cacgttgttc ccgctgataa cgctgccatt atgaagggtg atatcttccg
     6121 gagacagata cagtttcggc accatgactg tctgtccgtt gatggtgacg gactcccacc
     6181 acagcaggct tctggtcagt tgtgcaacct gcccggcggt cagcgacaca ccaaaggtca
     6241 gtccgagcgc tttctgttgt gctgccgcgc tgtccatcag gtatttcatc tgcgccaggt
     6301 cagagcctgt gccgttgata taacgtgagc ccgtctggtt cagtatgacg ttactgacat
     6361 accgggtatc aaaagccgca tcccccagaa agcggtaatc tttttccggt ttcaggccaa
     6421 gacggtcaag aatgtacgac gagcccagaa actgtttttc atcggtatat gccgggtctg
     6481 tttctcgtgg tgcctgtccg ggttgcatcc tgagcaggtc atacagtcca gcaaacagac
     6541 tgctgtccac ctttccgaga ccatccagtt tcgggttcac cgtaatcaga tactgactgt
     6601 ccgggtccgt ggacgggaca aaataaccat tgttaccgga tggcagggga tagttgctta
     6661 cacttgcccc ggacagcgac agcgcacccg ggatatcctt cagcgtatcc ggcagcgcgt
     6721 cattccatac agggccggtt atcccggtac cgctttcatt cagcagggct tcaccgctca
     6781 ccccctgact gaccgtcggt ggtgtcaggg agttcagcga aggcaccgtt atcgtgttac
     6841 ctgcaccacc tgcccggggc atgacagtgg tattactggt gtcagtggca aagctggcag
     6901 acacatcacc ggcagcgctg ataaccgcac ggtactgacc tgccggagag gtgccactgg
     6961 cttctgtcgc atccggcgag acctggcgat agaccttata ccatggctca gtaaaccacg
     7021 ctttggggag tgagccggag gcaagccgcc agaccgtttc ctttccggtg gtcccctcct
     7081 gccagccctg attgttcagt gtgtcgccac tcagggtcat actgccaccg gcgagaataa
     7141 acgatgcctc attatccagt cgtcctgcac agatatgcat atcagcgccg ccggatattc
     7201 tggcagcatg gccggaggca ctgactgtca ccgtttccgt atcgcccgta cggatacggt
     7261 attcatcagg taccgtggca cccttacccc agtacacggc cagatattcc acaccatcat
     7321 gccggacaac accccaccac cggttatttt ccggaaccgg tgaaatcacc ccgtgactgc
     7381 ttccaggaat gacctcatgg ctggccgaaa tggcatccca ggaattcagc agatgcgccg
     7441 tattcatggt gatatcgcca cgtgtggttt caatattacc ggaacggtta atgatttcgc
     7501 tgtttgccgt cccgctgct
//
LOCUS       DAZFFG010000181         7505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFFG010000181 DAZFFG010000000
VERSION     DAZFFG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.182"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            176..4429
                     /gene="fdeC"
                     /locus_tag="ACHJ7J_004617"
     CDS             176..4429
                     /gene="fdeC"
                     /locus_tag="ACHJ7J_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO5002739.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSSSLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMAVKVNNQLANGQSANQVTLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQATLEVDGSTPKVANDNDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSLTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSDVTLTASSENLVLDPKGTAKTNEQGQA
                     VFTGSTTIAATYTLTAKVEQANGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDANTATGEEAMK"
     gene            complement(4550..5401)
                     /locus_tag="ACHJ7J_004618"
     CDS             complement(4550..5401)
                     /locus_tag="ACHJ7J_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5002740.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLVNIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNSSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPI"
     gene            5659..6528
                     /locus_tag="ACHJ7J_004619"
     CDS             5659..6528
                     /locus_tag="ACHJ7J_004619"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5002741.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6688..7281)
                     /gene="rclC"
                     /locus_tag="ACHJ7J_004620"
     CDS             complement(6688..7281)
                     /gene="rclC"
                     /locus_tag="ACHJ7J_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO5002742.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7293..>7505)
                     /gene="rclB"
                     /locus_tag="ACHJ7J_004621"
     CDS             complement(7293..>7505)
                     /gene="rclB"
                     /locus_tag="ACHJ7J_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO5002743.1"
                     /translation="ATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDAIADIAR
                     KYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT         2045 a         1671 c         1931 g         1858 t
ORIGIN      
        1 cctgatgagt ggctacaggc actggatata ttaggtggcg gtgtactttc ttacataaag
       61 gtatttcctt ttctgcggaa aaggaaaccg ggaaatcccc ggtttttctg acaagcaggc
      121 gccattattt gtgtctgcct atgttcgtta attcgttcat caggaaatta tctcaatgtc
      181 acgttataaa acaggtcata aacaaccacg atttcgttat tcagttctgg cccgctgcgt
      241 ggcgtgggca aatatctctg ttcaggttct ttttccactc gctgtcacct ttaccccagt
      301 aatggcggca cgtgcgcagc atgcggttca gccacggttg agcatgggaa atactacggt
      361 aactgctgat aataacgtgg agaaaaatgt cgcgtcgttt gccgcaaatg ccgggacatt
      421 tttaagcagt cagccagata gcgatgcgac acgtaatttt attaccggaa tggccaccgc
      481 taaagctaac caggaaatac aggagtggct cgggaaatat ggtacagcgc gcgtcaaact
      541 gaatgtcgat aaagatttct cgctgaagga ttcttcgctg gaaatgcttt atccgattta
      601 tgatacgccg acaaatatgt tgttcactca gggggcaata catcgtacag acgatcgtac
      661 tcagtcaaat attggttttg gctggcgtca tttttcagga aatgactgga tggcgggggt
      721 gaacaccttt atcgaccatg atttatcccg tagtcatacc cgcattggtg ttggtgcgga
      781 atactggcgc gattatctga aactgagcgc caatggttat attcgggctt ctggctggaa
      841 aaaatcgccg gatattgagg attatcagga acgcccggcg aatggttggg atatccgcgc
      901 agagggctat ttacctgcct ggccgcagct tggcgcaagc ctgatgtatg aacagtatta
      961 tggcgatgaa gtcgggctgt ttggtaaaga taagcgccag aaagacccgc atgctatttc
     1021 tgccgaggtg acctatacgc cagtgcctct tctgacactg agcgccgggc ataagcaggg
     1081 caagagcggt gagaatgaca ctcgctttgg cctggaagtt aactaccgaa ttggcgaacc
     1141 tttggcgaaa caactcgata cggatagcat tcgcgagcgt cgggtactgg caggcagccg
     1201 ctatgacctg gttgagcgta ataacaacat cgttcttgag taccgcaaat ctgaagtgat
     1261 ccgtattgct ctgcctgagc gtattgaagg taagggcggt cagacacttt ccctggggct
     1321 tgtggtcagc aaagcaactc acggactgaa aaatgtgcag tgggaagcgc cgtcattact
     1381 ggctgaaggt ggcaaaatta ccggtcaggg tagtcagtgg caagtaacgc tcccggctta
     1441 tcgtccaggc aaagacaatt attatgcgat ttctgcggtt gcctacgata acaaaggcaa
     1501 tacctcaaaa cgcgtgcaga cagaggtggt cattaccgga gctggtatga gcgccgatcg
     1561 cacggcgtta acgcttgacg gtcagagccg tattcaaatg cttgctaacg gtaatgagca
     1621 aaaaccgctg gtgctgtctc tgcgcgacgc cgagggccag ccagtcacgg gcatgaaaga
     1681 tcagatcaag actgaactaa ctttcaaacc ggctggaaat attgtgactc gttccctgaa
     1741 ggccactaaa tcacaggcaa agccaacact gggtgagttc accgaaactg aagcaggggt
     1801 gtatcagtct gtctttacta ccggaacgca gtcaggtgag gcaacgatta ctgttagcgt
     1861 tgatggcatg agcaaaaccg tcactgcaga actgcgggcc acgatgatgg atgtggcaaa
     1921 ctccaccctg agcgctaacg agccgtcagg tgacgtggtt gctgatggtc agcaagccta
     1981 tacgttgacg ttgactgcgg tggactccga gggtaatccg gtgacgggag aagccagccg
     2041 cctgcgattt gttccgcaag acactaatgg tgtaaccgtt ggtgccattt cggaaataaa
     2101 accaggcgtt tacagcgcca cggtttcttc gacccgtgcc ggaaacgttg ttgtgcgtgc
     2161 tttcagcgag cagtatcagc tgggcacatt acaacaaacg ctgaagtttg ttgccgggcc
     2221 gcttgatgca gcacattcgt ccatcaccct gaatcctgat aaaccggtgg ttgggggtac
     2281 agttacggca atctggacgg taaaagatgc ctatgacaac cctgtgacca gcctcacgcc
     2341 ggaagcgccg tcattagcgg gtgccgctgc tgtaggttct acggcatcgg gctggacaaa
     2401 taatggtgat ggaacgtgga ctgcgcagat tactctcggc tctacggcgg gtgaattaga
     2461 agttatgccg aagctaaatg gacaggatgc ggcagcaaat gcggcaaaag taaccgtggt
     2521 ggctgatgcg ttatcttcaa accagtcgaa agtctctgtc gcagaagatc acgtaaaagc
     2581 cggcgaaagc acaaccgtga cgctggtggc gaaagatgcg catggcaacg ctatcagtgg
     2641 tcttgcgttg tcgtcaagtt tgacggggac cgcctctgaa ggggcgaccg tttccagttg
     2701 gaccgaaaaa ggtaacggtt cctatgttgc tacgttgact acaggtggaa agacgggcga
     2761 gcttcgcgtc atgcctctct tcaacggcca gccagcagcc accgaagccg cgcagttgac
     2821 ggtcatcgcc ggagagatgt catcagcgaa ctctacgctt gttgcggaca ataaggctcc
     2881 gaccgtcaaa acgacgacgg aactcacctt caccgtgaag gatgcgtacg ggaacccggt
     2941 caccgggctg aagccagatg caccagtgtt tagcggtgcc gccagcacgg ggagtgagcg
     3001 tccttcagca ggaaactgga cagagaaagg taatggggtc tacgtgtcga ccttaacgct
     3061 gggatctgcc gcgggtcagt tgtctgtgat gccgcgagtg aacggccaaa atgccgttgc
     3121 tcagccactg gtgctgaacg ttgcaggtga cgcatctaag gctgagattc gtgatatggc
     3181 agtgaaggtt aataaccaac tggctaatgg acagtctgct aaccaggtaa ccctgaccgt
     3241 tgtggacacc tatggtaacc cgttgcaggg gcaggaagtt acgctgactt taccgcaggg
     3301 tgtgaccagc aagacgggga atacagtaac aactaatgcg gcaggtaaag cggacattga
     3361 gctgatgtca acggttgccg gggaacacag catcacggcc tcagtgaata atgctcagaa
     3421 gacggttacg gtgaaattca aggcggattt cagtaccggt caggcgaccc tggaggttga
     3481 tggcagcacg ccaaaagtgg caaacgacaa tgatgccttt acgctgacgg caacggttaa
     3541 ggatcaatac ggcaaccttc tgcctggcgc tgtggtcgtc tttaatctgc ctcggggcgt
     3601 caaaccgctt gcagacggta atatcatggt gaacgccgac aaggagggta aagcggaact
     3661 gaaagtggtc tccgtgactg ccggaacgta tgagatcacg gcgtcggcag gaaatgacca
     3721 gccttcaaat gcgcagtctc taacgtttgt ggccgataag actacggcga ccatctccag
     3781 tattgaggtg attggcaacc gtgcagtggc ggatggcaaa accaaacaga cgtataaagt
     3841 tacggtgact gatgccaata acaacctgtt gaaggatagc gacgtgacgc tgactgccag
     3901 ctcggaaaat ttagttctgg atcctaaagg gacggcgaaa actaatgagc aaggacaggc
     3961 tgttttcacc ggctctacca ctatcgcagc gacatataca ctcacggcga aagtggaaca
     4021 ggccaacggt caggtatcga cgaaaactgc tgaatctaaa ttcgttgcgg atgataaaaa
     4081 cgcggtactc accgcatcat ctgatatgca atctctggtg gcggatggga aatcgactgc
     4141 gaagctggag gtgacactga tgtcggcaaa caaccccgtt ggggggaata tgtgggtcga
     4201 cattcagacg ccggaagggg tgacggagaa ggattatcag ttcctgccgt cgaaaaatga
     4261 ccatttcgtg agcggaaaaa tcacgcgtac atttagtacc agcaagccag gtgtctatac
     4321 attcacattt aacgccctga cctatggcgg gtacgaaatg aagccagtga cggtgaccat
     4381 taccgcggtg gatgccaata cggcaacggg cgaggaggcg atgaaataat attaagagta
     4441 tgaaagtaac attctaataa acaataatgg cgttgacatc ttcaacgcca ttatttattg
     4501 aatttcctga cagtagtctc gttataaatt catttagaca tagcgacagc taaatcggac
     4561 gaacgggaat aaaaatttcg caaacaacat cattgccatc cttttctacg aaatcttcat
     4621 tgtaataaaa gcgctcaata tcgctgctgt tcactctggc taaccccctg gcaggcaact
     4681 ctatcagata gatgttacgc acccagtcag gataattttc ccagtttcct tcataacgga
     4741 aagaggcata aagtcctcgg gtcgttgtcc actcttttgg catgtcagca tattcacata
     4801 ccgtaccagc aaaagtttca accgtgagca aatcgaccga ttttgttgac ggttcaaacg
     4861 tggagacgaa atagatatcc gtcttaaaat tattcataca tctggctaaa tgatgacgtc
     4921 gcagtttgac ttcttcatcc ggtgatttat tcgacacttc aatatcatat ttaaatgaat
     4981 tgccaaaaat aggtgtctca ggaaagttga ctaatctaca ctccgtcttt tgctgttgac
     5041 gtattaaaaa agaagggaag atatttacga gatcccaata atcacggtgg cggtatacac
     5101 ggggagagca gccgaataac tttttaaatt cgcggctgaa tgactgctgt gaatcaaaat
     5161 gcaaagagag tgcaatatca agcatagatt tcgcggtgag ccggacaaga atggcggcac
     5221 gacaaagcct tcgtttgcga atgtattctc ccaaaggcac atgcatgaaa tttcggaaca
     5281 gaagctggat gttgcgtctg ctgtagccag atttctgtgc gatatcttcg attgaaatag
     5341 ggtgctcaag attgcactca atccactcca ggagctgctg tagaatcttc tgcctgatca
     5401 tcacattctc cgttaattct ttgtcatatc gcacattttc gttatgccct gggccctaag
     5461 tgtatcaaaa acagcaaggg cgaaggcaaa agcactggta gcgtctgctt cgttgtcccc
     5521 gaaaatagag aggtttcagt cctacattat taatgaattt tttgcataag tgatatcaaa
     5581 atccacatac taatttgagg ttacgtttta acgtagactc attgttcatg cctaatgtgc
     5641 ctaatggagg gactgacagt ggaattttcc gtattaagta acaatctgaa aatgccgatg
     5701 atgggatttg gtgtttttca ggttaccgat aaaaacgtgt gcaaacagtc agtgttgaac
     5761 gctatccgca cgggttatcg actcatcgac actgcagcgg tatacggtaa tgaagatgct
     5821 gtcggtgaag ccgttcgtga agccatatcc gaaggtttat gtacccgaga agagttattt
     5881 ataacgtcta aattgtgggt gcaggatatg ttgaatcagg atacggcagc agcaggtatt
     5941 gaagcatcat taaaaaaatc gggactagaa tatttcgacc tctatttatt gcatcaggct
     6001 atgcgcgatt atttcagtgc gtggcgtgca ctcgaagatg cctatgaaga gggcaaatta
     6061 aaagcgattg gggtttccaa tttctatcct catgttctgg cgaacttttg tgaaacggta
     6121 agagttaaac cgatggtcaa ccaggtcgag ttgcatccct attttgccca accagaggcg
     6181 ctggcaacca tgaagtatta taacgtgcag cctgaagcat gggctccgtt aggtggtgga
     6241 cgacataaac cctttgaaaa taatctgctt cagagtattg cagatgccca tcaaaaatcg
     6301 atttctcaag tcattctgcg ttggaatatt caacggggag tggtcgttat tccgaaatcg
     6361 acacatcaac agcgtatcga agaaaatttt gctatctggg atttctcact gacagagaaa
     6421 gaaatggcac aaattagttc gcttgatttg ggttatgttg gggagtcggt aaaacatttt
     6481 aatcctgaat ttgttcgtgg ttgtcttgct gtaaaaatac atgattgata ttaatcatgt
     6541 attttacctg agacgacaag aatcttttaa caggggagtg atattgatct tcactctgtc
     6601 atatctccgg taatatggcg tcaggcttca tcatcagaaa gggggcctga cgcctgaaaa
     6661 agtgaacaac agacagtgtt cggattatca atattcagtt tttaacgttg aactggattc
     6721 attactgcgt tgtttaagaa tttcccgcgc cgaatctgcc attatcatga cagcacctgc
     6781 cagcatcaga gtatctttca ataccaagcg accagcaccg gataaataag ggaaaccatg
     6841 atgagcgtca cccaatgcgg gtacccatgc ctccggggtg gtgattaaaa atgagagtgt
     6901 taccaacggt gtggtaaatg ccatcagccc tcccaataaa cctaaccagc gattgacagg
     6961 attagccaaa accagcagag caataatcac ctccacgacg ccaagaccgt tggaaaaacc
     7021 ataggtatta ttggccgatt gccatgccct tgcttctggt ttgtattcgc cttcgtgagt
     7081 cagatactgt ttatagtctt ccgggtgttc ataaaagaac gacattagtg gactgtttgc
     7141 gacgaatggt gtaatgctgt ctgcctcgta agggacaaac tttaataacc caatccacat
     7201 aaaaacaatc gcaatactca gacgaatcaa tgtcaggcca attttatctc cccgacttaa
     7261 caggtgcagg tatttttcca ttgtgaacat ccttatttat acaatactgc tgttgcggtt
     7321 gaattattat caattcgcat cgatgtgact ttccaggatg aagcattata ttttctggct
     7381 atatcagcaa tggcatcctc cagaactgtc ggtgatgctg atactgacga gacgctgata
     7441 tgtttcagaa ttattttatc atctgcattg gtggaaaatg ccataacgcc agataatagt
     7501 gttgc
//
LOCUS       DAZFFG010000182         7447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFFG010000182 DAZFFG010000000
VERSION     DAZFFG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.183"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..2894
                     /locus_tag="ACHJ7J_004622"
     CDS             <1..2894
                     /locus_tag="ACHJ7J_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5002744.1"
                     /translation="ALVYSSLYAGSGSMVTVGAGTQNIDGLGFNGGTVDFGKIAPGEK
                     QSNNMVHVSSLDLQGGGAVQVDASGDVIADTVSRDVNTFLPLLEQDDKNAAIQLVSVS
                     ENGREVVGSAGGLQLQDQSGQRITDSIQHAVIQNGQKVADGTYDYRLTSGTNNDGLYI
                     GYGLTQLNLLTSGTDALELDANGKTGNAADMSARITGTGDLAFNSQKGETVSLSNQNN
                     DYTGVTDIRGGHVLMNSNSALGQTSEIRLAADTMLDMNGHSQTAGKLNGTAGSVLNIN
                     GGNLTLTEGGVSAGILSGNGALNVSGGVLDITGASSTFTASTTIAKDAVVRMNDVSGL
                     GTGNISNAGTLSLTHASGSLGNNLSGTGTVSLLSSDTQLSGNNSGYSGLFVVDESSQL
                     TASATENLGTASVNNSGTLVLNSATDWQLTNDVSGSGNFRKTGSGSLTVGNNAAWTGQ
                     TDIDAGTLILGKTDSPVMLASSQVNIAENGTLSGFGGVTGNVTNSGTLDLRADAPGNV
                     LTVGGNYTGNNGTLLMNTVLGDDSSATDKLVIKGDASGQTRVEVTKAGGTGAQTLNGI
                     ELIHVDGNADSAEFVQAGRIAAGAYDYTLGRGQGSNSGNWYLTSGKNTPEPTPTPDPD
                     SRPEPAPGGYDNDLRPEAGSYTANMAAANTMFVTRLHERLGPVQYTDVMTGETKNTSM
                     WMRHEGGHNRWRDGTGQLKTQGNRYVVQLGGDIAQWGRGETDRWHLGVMAGYGNDHNN
                     TDSVRTGYRSKGSVNGYSTGLYATWFANDETHNGAYLDTWVQYGWFDNHVKGDGLPGE
                     SWKSKGLTASLETGYTWKAGEFSGSHGSLNEWYVQPQAQVVWMGVKADEHRESNGTRV
                     ESTGDGNVRTRLGVKTWIKGHNRMDDGKSREFRPFVEVNWLHNTRDFGTRMNGVTVHQ
                     DGARNIGEVKAGVEGQINDRLNLWGNVGVQAGDKGYSDTSAMLGVKYTF"
     gene            3393..3982
                     /locus_tag="ACHJ7J_004623"
                     /pseudo
     CDS             3393..3982
                     /locus_tag="ACHJ7J_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4068..4427
                     /locus_tag="ACHJ7J_004624"
     CDS             4068..4427
                     /locus_tag="ACHJ7J_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026358148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002745.1"
                     /translation="MSFSTRFLQLRKEHGLTQPQMADKVGIHLTQVRRYESGEAQPSL
                     EILKRIAVTFIVSADWLIFEDGEREPQDELKLKFEAIKQMDEEELKSVTSVLDALILK
                     HQARRVLSYIYTMSGRA"
     gene            complement(4545..4748)
                     /locus_tag="ACHJ7J_004625"
                     /pseudo
     CDS             complement(4545..4748)
                     /locus_tag="ACHJ7J_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4854..5129)
                     /locus_tag="ACHJ7J_004626"
     CDS             complement(4854..5129)
                     /locus_tag="ACHJ7J_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5002746.1"
                     /translation="MELKWTSKALSDLARLYDFPVLASKPAAARTVQSLTQAPDILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWRTRENR"
     gene            complement(5129..5413)
                     /locus_tag="ACHJ7J_004627"
     CDS             complement(5129..5413)
                     /locus_tag="ACHJ7J_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO5002747.1"
                     /translation="MQMKNNTTQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQVWADSLSSDNPLPVPR"
     gene            5743..5994
                     /gene="tnpB"
                     /locus_tag="ACHJ7J_004628"
                     /pseudo
     CDS             5743..5994
                     /gene="tnpB"
                     /locus_tag="ACHJ7J_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            6001..>7447
                     /locus_tag="ACHJ7J_004629"
     CDS             6001..>7447
                     /locus_tag="ACHJ7J_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO5002748.1"
                     /translation="MNIRIRSGILPCMDISALNTTNDIEKLRAMALAMVQKVMSENAE
                     KERELQEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAELTAHLD
                     KLLPQSPEEDEKAPRSRPIRKPLPVHLPRVEKIIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVITQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMAAMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEDLWSWLEEQEPQCSPGRALHKAIVYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGE"
BASE COUNT         1924 a         1781 c         2018 g         1724 t
ORIGIN      
        1 cagcactggt ttattcatca ctgtatgcag gttccggcag tatggtaaca gtaggggccg
       61 gaacccaaaa tatcgacggg ctgggattta atggtggcac tgtggacttc ggaaaaatcg
      121 ctcccgggga aaaacaaagc aacaacatgg ttcacgtttc ttctcttgat ttacagggtg
      181 gcggtgcagt tcaggtggat gcatccggcg atgtcatcgc tgatactgtt tccagagatg
      241 tgaatacatt tttgcccctt cttgagcagg acgataaaaa tgcagctatc caactggtct
      301 ctgtatctga aaatggtcgg gaggttgtcg gctctgccgg tggactccag ttacaggacc
      361 agtccggaca acgcattaca gacagtattc agcatgcagt catacaaaat ggacagaaag
      421 tggcagacgg aacctatgac tatcgcctga ccagtggtac caataatgac gggctgtata
      481 tcggttatgg cctgactcag cttaatttgc ttacttccgg tacggatgca ctggagcttg
      541 atgcaaatgg taaaaccgga aatgccgctg acatgagtgc ccgtattacc ggtactggcg
      601 acctggcatt caacagtcaa aagggcgaaa ccgtttctct ttcaaatcag aacaatgatt
      661 acacaggagt gacagatatt cgcggcggtc atgtcctcat gaacagcaac agtgctctgg
      721 gacagaccag tgaaattcgt ctggctgcag acactatgct ggatatgaac ggtcacagcc
      781 agactgccgg taaactgaac ggtactgcag gctctgtact gaatatcaat ggcggtaatc
      841 tgaccctgac agaaggcggt gtgtctgccg gcatcctctc tggcaatggt gcactgaatg
      901 tcagcggtgg tgtgctcgat attacggggg ccagcagcac ttttactgcc agcaccacaa
      961 ttgcaaaaga tgctgttgtc cgcatgaatg atgtttccgg actgggaacc ggtaacatca
     1021 gtaacgccgg gacattgtcg ctgactcacg cctccggttc gctgggcaat aatctgagcg
     1081 gtaccggtac agtatccctg ctcagcagtg atacacagct ttctggaaac aacagtggtt
     1141 attcagggct gtttgttgtg gatgagagtt ctcaactgac tgcatcagca acagagaacc
     1201 tgggcaccgc atccgtaaac aacagcggaa ccctggtgct gaacagtgca acagactggc
     1261 aacttacaaa tgatgtcagc ggaagcggta atttccgtaa aaccggctct ggttcgctga
     1321 ccgttggcaa taatgcagcc tggaccgggc agactgatat tgacgcaggt acactgattc
     1381 tgggtaagac ggattcccct gtcatgctcg ccagcagcca ggtcaatatt gctgagaacg
     1441 gaacactttc cggctttggt ggcgtcaccg ggaatgtgac caacagtggt actcttgacc
     1501 tgagagctga tgctccgggt aatgtgctga ctgttggcgg caactacacc ggtaataacg
     1561 gcacgctgct catgaacacc gttctgggag atgacagctc cgcaacggat aaactggtga
     1621 ttaaaggtga tgcatccggt cagacccgtg tggaagtcac taaggccggc ggtacaggtg
     1681 cacagacact caatggtatt gaactgattc atgtcgacgg taacgccgac agtgctgaat
     1741 ttgttcaggc cggtcgtata gctgccggtg cttatgacta cacgctgggg cgtggtcagg
     1801 gaagcaacag tggtaactgg tatctgacca gcggtaaaaa tacgccggaa ccaacaccga
     1861 cgcctgaccc ggactccaga cctgaacctg cgccgggtgg ttatgataat gacctgcgtc
     1921 cggaagctgg ctcctacacg gcaaatatgg ctgcagcgaa caccatgttc gtgacccgtc
     1981 ttcatgaacg tctggggccg gtgcagtaca ccgatgtcat gaccggtgag acgaaaaata
     2041 ccagcatgtg gatgcgccac gaaggaggac ataaccgctg gcgtgatggt acaggtcagc
     2101 tgaaaacaca gggtaaccgt tatgttgttc aactgggggg agacattgca cagtggggcc
     2161 ggggagaaac tgaccgctgg caccttggtg ttatggccgg ttacggtaac gaccataaca
     2221 atacggactc tgtgcgcacc ggataccgct caaaaggcag tgtgaacgga tacagcacag
     2281 gtctgtatgc cacctggttt gccaacgatg aaacacataa cggggcttat cttgatacgt
     2341 gggtacagta cggctggttt gacaaccatg tgaaaggtga cggactgccg ggcgagtcct
     2401 ggaaatcaaa aggcctgact gcttcgctgg aaactggtta tacatggaaa gcaggtgagt
     2461 tcagcggcag ccatggcagc ctgaatgaat ggtatgttca gcctcaggca caggttgtct
     2521 ggatgggtgt aaaagcggat gaacaccgtg aaagcaacgg tacccgtgtt gagagtaccg
     2581 gtgacggtaa cgtccgtacc cgtctgggtg tgaaaacctg gattaaggga cacaacagaa
     2641 tggacgacgg taaatcccgt gagttccgtc cgttcgtgga ggtgaactgg ctgcacaaca
     2701 cccgtgattt tggaacccgc atgaacggcg tgacggtaca tcaggacggt gcccgcaata
     2761 tcggggaagt gaaagccggt gttgaaggac agataaatga ccgtctgaac ctgtggggta
     2821 atgtgggagt acaggcgggt gacaaggggt acagcgacac ctcagccatg ctgggtgtga
     2881 agtacacttt ctgagtacgg tgaatatctg acagacagtc gcggccacgg acggcagcga
     2941 ctgtctgata catggtccat gtcttacagt ttactgataa tgttttgact gaaaatgaat
     3001 aacagtaatt attactgaag ggttcaggtc cagtttacgg gtaatatgcc gtatgggcca
     3061 tactataagg tggtaataac caggtaacac cttgtgtacc gcatgtgcag gcatagtctc
     3121 tctcctgtgt aaaaatccag gcgacaacta tcctgacaca ggggggactc accggtctcc
     3181 ttcacagtaa tacgcactca tggcctttac ggcagtatca ataagacttt attttcagtg
     3241 agtcataccc tcgcagtcga tagtttatta cggatacctg ccagcagaga ataaacagac
     3301 cattgatcgg catagggggc agaacaaggc tgcgcccctg ccacaccacc cggcatgtgg
     3361 gtccgcaccg ggcggttcga gaagttgagg ttatgcgagt cgagggaagc ctaaagcatc
     3421 aatccacact ggaacagacg accgaaaatc aacgtcggga aatcgaaagc gcgggtttta
     3481 atgtcagacc ccaacgaata atcgaagaac aaattagtgg ctcagtagca gccagcgaac
     3541 gtcctggttt taaccggttt cttgatcgtc tggaaagtgg cgatgtattg atagtcacaa
     3601 aactggatcg ccttggtcgt aatgcaatgg atataaggaa aacagtggag caactgaccg
     3661 aatctggcat cagggtgcat tgcctggcat taggtggtgg gttctgacca gtccggcagg
     3721 aaaaatgaca atgcaggtga tttcagcggt cgctgaattt gaacgagacc ttttacttga
     3781 acgaacttat tcaggggtag caagagcacg ggatgcagga aaacgttttg gtcgcccacc
     3841 ggtattaaat gaagaacaaa cacagaaggt actcgaacgc attaagtctg gggcaagtat
     3901 aagcgctatt gctagggaat ttcgaaccac acggcaaaca attctgagag ttagagcgaa
     3961 acagcaaact gttactgtat aaaaaatagt cttggcgtgg gatattttcc gttttccaag
     4021 cggaccccat aatgggtcac aacattgatt atcagaaggt gttctggatg agcttttcaa
     4081 cccggtttct gcaacttcgt aaggaacacg gactgaccca gccacagatg gcggataaag
     4141 tgggtatcca tttgacacag gttcgccgct acgagtcggg tgaagcacag ccctctttag
     4201 agatccttaa gcgcattgcc gtaactttta tcgtgtcagc cgactggctg atatttgaag
     4261 acggtgaacg cgagccacaa gatgaactga agctgaagtt tgaagcaatt aaacagatgg
     4321 acgaagagga actgaagtca gtgacatcgg ttcttgatgc gctcatctta aaacatcagg
     4381 cccgtagagt gttgagttac atttatacaa tgtccggcag ggcatagaag gggtgaaaga
     4441 aaatacctag gcgaagccgc tgaacatcat tctggcgatt tcaataaagg cacttgctcg
     4501 cgctctgtca ttttctgaaa atcttcatgc tgcatttcga ttttccttct aagatcataa
     4561 ctcgcatacc tgttcttttt atgtatatcc atatcgattt ttgtgacgag ttcaaaccac
     4621 aatgtgcgca ctccccatgc agcgttatga aacatttccg caacggctat agaatctgta
     4681 ttaactgctt cggtcatgta gtcgctaata cgttctgcgg taattttcgt tatttcatcg
     4741 taattcattt tatctcctat aagattattt aatggaatgc aaaatatctg gcctgataat
     4801 aatgttccag aattaatatc tgcaggctat gttaagttgt ccccacacac aatctacctg
     4861 ttttctcgtg tgcgccacag gcgcaataca taaatagtct ggccattaag ttcgtaacgg
     4921 atttcgtact cgccagcaaa aatccgtctg acctccctgg gttcaaactg aaacaactgt
     4981 tctcccatac gtggatgagt taacagaatg tccggtgcct gtgtcaggga ttgcaccgtt
     5041 ctggcggcag caggtttact ggccagcacc ggaaaatcat ataaccgcgc caaatcagaa
     5101 agagccttac tggtccactt cagttccatc agcgtggcac cggtaacgga ttgtcagaac
     5161 tgaggctgtc cgcccagacc tgtacagcct gatggtcgat aacctgtccg gatgtcacat
     5221 cgtccagggc ttccagcgtc aggcgattac gctcctcctc ctgggcaagc catgcagaaa
     5281 gcgcctgttt gataacccag ccccgggagc gttccagtct ggcggccatc tggtcgactt
     5341 tatcagccat aggtaatggc acatgcgcgg taattacttt tgttgcttgt gtggtattgt
     5401 ttttcatttg catatccgat tagttgttaa tcatatttaa tcatagtgat tactttcaga
     5461 cggaacaagg taaataatgc gcgtcagcgc atgtaaggcg gcatacggag gttacatgta
     5521 gcacaggtgt aaaaccgccg aagcccggcg taagccggta cggtcgtccg aatatcacca
     5581 gtcccactcc ggccctgcga ggccatgtgg cctgtcagcg atgaaaatgc ctctgtgcgc
     5641 ggtcggagtg gtcccgacaa gggtttaccc gcagtcgggg cgtatctccg cgttagcggg
     5701 ccgtgagggc cgcttacgag cgtgtatgag agacgtacat tggtttcttc tcgcaaagtt
     5761 ctccggtggg ataaacacgg ggtgtggttg tgtacccgcc gtcttcacaa aggcagcttc
     5821 cgctggccac gtgcaaatga cgctgcctgg cacctcactc ccgacgagtt taactggctg
     5881 attgccggtg ttgactggca gcaggttaag ggacatgacc tgacgaaatt ggtctggcag
     5941 aatgaacctg aactgctccc tgaaaacacg caaaatacac tactaactaa ctgaaaaata
     6001 attaatattc gtatacggag tggtatactc ccctgtatgg atatctccgc tctcaacacc
     6061 acgaatgaca tcgaaaaact gcgtgctatg gcacttgcca tggtacaaaa agtcatgtcg
     6121 gagaatgccg aaaaagagcg ggaattacag gagaaaagcc ggcgcatcca gcttctggaa
     6181 gaaatgctga aactggttcg tcaacagcgc ttcggtaaaa agtgtgagac gctggcaggt
     6241 atgcaacgct ccctgttcga agaggatgtt gatgccgata tcgccgagct caccgcacat
     6301 ctggataaac tgctcccgca atcccctgaa gaagacgaaa aagcgccccg ttcacgcccg
     6361 atacgcaaac ccttaccggt tcatcttcca cgggtggaaa aaattatcca gccggacact
     6421 gaccattgcc ctgaatgtga cgagccgctg cactatatcc gtgatgcggt gagtgaaaag
     6481 ctggagtata ttcccgctca ctttgtggtg aaccgttatg tccgtccgca atacagttgt
     6541 ccctgttgcc agaaggtgtt cagcggtgaa atgccggcac atatcctccc gaaaagtgcc
     6601 gttgagccat cagtcatcac acaggtgatc atcaataaat acggtgacca cctgcctctg
     6661 tatcgccagc aacaggtctt tgcccgttca gatgtcgggc tgcccgtcag ttcgatggct
     6721 gccatggttg gcgcggcggg tgctgcatta tctcccctgg cggcgttact ccatcgcgag
     6781 ttgataaacc gtccggtggt gcatgcagat gagactaccc tgaagatcct gaacacgaag
     6841 aaaggcggta aatcctgctc cggttatctg tgggcatacg tcagtggaga aaggacggga
     6901 ccgtcagttg tgtgcttcga ctgccggacc ggacgtagcc atgagtatcc tgaaaactgg
     6961 cttcagggct ggggcgggac gctggttgtc gacggacata aagcttaccg gactctggca
     7021 aacaaagtgc cggagatcac gctggccgga tgctgggccc atgcccgcag gggcttcgcc
     7081 gacctgtata aaatcagtaa agatccacgg gctgccatcg ccgtgaagaa aatcgcgggg
     7141 ttgtaccgtc ttgagaagaa gatcagtagc cgccccgtgg aaaaaatccg ccagtggcga
     7201 cagcgttatg cccgtccgat actggaagat ctgtggtcat ggcttgaaga gcaggaaccg
     7261 caatgttctc cgggaagggc attacacaaa gccattgtct atgcgctgtc tcatcgcgtg
     7321 gaactgagcc gcttcctgga agatggtgcg gtgccgctgg ataataatgt gtgtgaacgg
     7381 gccatcaaaa acgtggtcct gggcagaaaa tcgtggctgt tcgccggttc gcagatggca
     7441 ggagaac
//
LOCUS       DAZFFG010000183         7349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFFG010000183 DAZFFG010000000
VERSION     DAZFFG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.184"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(137..421)
                     /gene="ppnP"
                     /locus_tag="ACHJ7J_004630"
     CDS             complement(137..421)
                     /gene="ppnP"
                     /locus_tag="ACHJ7J_004630"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO5002749.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(493..1170)
                     /locus_tag="ACHJ7J_004631"
     CDS             complement(493..1170)
                     /locus_tag="ACHJ7J_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO5002750.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLELSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1428..1619)
                     /gene="yaiA"
                     /locus_tag="ACHJ7J_004632"
     CDS             complement(1428..1619)
                     /gene="yaiA"
                     /locus_tag="ACHJ7J_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO5002751.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(1669..2193)
                     /gene="aroL"
                     /locus_tag="ACHJ7J_004633"
     CDS             complement(1669..2193)
                     /gene="aroL"
                     /locus_tag="ACHJ7J_004633"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO5002752.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMKNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2376..2834)
                     /locus_tag="ACHJ7J_004634"
     CDS             complement(2376..2834)
                     /locus_tag="ACHJ7J_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO5002753.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2954..3763
                     /gene="proC"
                     /locus_tag="ACHJ7J_004635"
     CDS             2954..3763
                     /gene="proC"
                     /locus_tag="ACHJ7J_004635"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO5002754.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(3780..4895)
                     /gene="adrA"
                     /locus_tag="ACHJ7J_004636"
                     /gene_synonym="dgcC"
     CDS             complement(3780..4895)
                     /gene="adrA"
                     /locus_tag="ACHJ7J_004636"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO5002755.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTAIKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVMLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(4997..5317)
                     /gene="psiF"
                     /locus_tag="ACHJ7J_004637"
     CDS             complement(4997..5317)
                     /gene="psiF"
                     /locus_tag="ACHJ7J_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO5002756.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5436..6851)
                     /gene="phoA"
                     /locus_tag="ACHJ7J_004638"
     CDS             complement(5436..6851)
                     /gene="phoA"
                     /locus_tag="ACHJ7J_004638"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO5002757.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(6952..7212)
                     /gene="iraP"
                     /locus_tag="ACHJ7J_004639"
     CDS             complement(6952..7212)
                     /gene="iraP"
                     /locus_tag="ACHJ7J_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO5002758.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYAVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1795 a         1920 c         1751 g         1883 t
ORIGIN      
        1 aatgatattg atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag
       61 caaaggaaat attattatgg gttaaagaga ggatatttca gaaaaatgcc ctcccgttca
      121 aggggaaggc gaggaattac agatagcggc acagataaga ggtgggttcg gcaacttgca
      181 gatgaaactc actgtgaccg ggaacattaa acaccgaacc ggcttcatac acttgccagt
      241 cggtcgcgtc aggcagtaac acattcagcg cgccactgat taccgtcatt tcttccggct
      301 cagcggtgct gaaggtgtat tcgccttcaa ccataacacc cacgctggcg cgaccagtgc
      361 tgctgctgga aaagccgatc gatttcactt tgccggaaaa gtactcatta ctttgaagca
      421 taaactggcc ctgtctggta gttgaacatc tacttcacta taggggccag atgcgctggc
      481 tgtcacgcaa aattacacca gtaattccgc agccagccgt gcaatcaata cgttagagag
      541 caagacagga acatcgagct gtttttgcag taaatcgcga tgacgctggt taaatcccaa
      601 acaatccagc atgatgacat ctgcgccttt cgccagtaat tctttcccgg catcaatgat
      661 tttttgttct gaatcatgaa tggggttacc caatgaaaat accggcggtt tctgcaaaat
      721 ttgccatttt tgcgcctgaa cggtcagcaa ctcctcaacc ggtacgataa cccccacctg
      781 atgatcttca acaatagagg aaaccagcgg tggcaatatc cgcgacagct caagaaagat
      841 cgtattacgc gcagtcatac tactaatgtt tgccgtactc attaatataa tgacgtcgta
      901 gccctgatta tcgagcactt caaccacacc ttgcaggtca cgctctactt tgcgacgcga
      961 aacatgggcc agatggttgt catttaataa tgtgagaatt gtgtcttcgc ctgcttctgg
     1021 cgcgtactcc gccatcactt cttcgcggct taacttcccc agcaggctat gatgggaaat
     1081 attatcttcg tcaatgtatt ctgtcaggag cggcaaaact tcctgcatag gtacaatgcc
     1141 gatggttaga atcgccaacg acgcactcat tgttcttcca ccttactttt atttcgtcgt
     1201 taattaccgg gcaagtgtta agcaccactg ctgacactta cttgtgctac aaatgacaaa
     1261 aagcgtagca gcagacgtgc acggcataat gtaaagattc caaatgattc cagtaatgga
     1321 ttgttattgt ttaatattct aattatttga aaaacatgaa ttatgaaaaa tgtgacgcag
     1381 atcccttgta gtggcactca gaatcccttc cgagcagtct gatgcggtca ctctttgtca
     1441 gggtcctcat agcgtttttt cgcatccatc gcttcctgag cggtcggatg ttcactgatc
     1501 aacgagtctg gtttaggatg atcggctctg agttggtacc aggttaccgt ctgtcctggc
     1561 tttccttttt caatcgtcac tatatatgct tcacgaggat aaggcggttt cgttggcatc
     1621 gttcttcctt atttcacggg atgaacgtta agtataggcg ctcgaaaatc aacaattgat
     1681 cgtctgtgcc agggcgctgc gaatttcaga aatcacctgg ctgggttcgt ttgttgcgtc
     1741 gatgataata tgcgcaactt cgcgatatag cgcatcgcgt tcttccagca cttcctgaac
     1801 ttcttcgctc agcggttttc ccgttaaggt tggccgtaaa tcttcttccg gtgcagcttg
     1861 cagtcggtta accaggactg atactggcgc acacaaataa accacgatcc cgttattttt
     1921 catgaagtga cgattaaatt ccgtcagaat aatgccgccg cctgtagcga taacggtgga
     1981 tggcgcagtt accgcttcca gcgccgccgt ttctctggcg cgaaatcccg cccactcttc
     2041 cctttcgacg atctccgcga ccgtcatatt gagctgtgat tgcaaccact gatcggtatc
     2101 gacaaaccga cggttaagcg aatcggcaag ggccattccg accgttgttt taccacagcc
     2161 ccgaggcccg atcagaaaaa gaggttgtgt catcgtgggt tttccccaat aggtcgcaat
     2221 gcggcgaaag ccggtgtcat gagaatagcg atcataccat caaactagta caatttcgat
     2281 tgtaaagaaa aaattccact taaagtgaaa atctcaatac atcccttact ataccaataa
     2341 atattcaaga atgaagtgta aataataaat tacatttagc cacgactacg ttgcacttcc
     2401 agccaccact tctccagctc cgcggcaaag gcctggcggt cacgttgtga aaggctatct
     2461 ggtccgccgg tctggatccc actggcacgt aaggtatcca taaaatcgcg catcgtcagg
     2521 cgctcacgaa tggtcgctgg cgtgtaacgt tcaccgcgcg gattaagcgc cgcagcgcct
     2581 ttctcgatgg cttcagcagc caaaggtata tctgcggtga tcaccaaatc gcccgcttca
     2641 cactgccgga caatttcgtt atcggcaacg tcgaaacctg ccgcgacgcg cagcgtacga
     2701 ataaatcgcg atggcggcac gcgtaaactc tggtttgcta ccagtaccag cggcatctgc
     2761 atacgttccg ccgcgcgata caaaatctct ttaattacat tgggacacgc gtcggcatcc
     2821 acccaaattg tcataaagtc atcctttgtt gggtaatcct ctattgtgtc gcgcttttgc
     2881 cttccggcat agttctgttt atgcttctgc cagcgattat caaaacaatg aatttcacgg
     2941 caggagtgag gcaatggaaa agaaaatcgg ttttattggc tgtggcaata tgggaaaagc
     3001 cattctcggc ggtctgattg ccagcggtca ggtgcttcca gggcaaatct gggtatacac
     3061 cccctccccg gataaagtcg ccgccctgca tgaccagttc ggcatcaacg ccgcagaatc
     3121 ggcgcaagaa gtggcgcaaa tcgccgacat catttttgct gccgttaaac ctggcatcat
     3181 gattaaagtg cttagcgaaa tcacctccag cctcaataaa gactctctgg tcgtttctat
     3241 tgctgcaggt gtcacgctcg accaacttgc ccgcgcgctg ggccatgacc ggaaaattat
     3301 ccgcgccatg ccgaacactc ccgcgctggt taatgccggg atgacctccg taacgccaaa
     3361 cgcgctggta accccagaag ataccgctga tgtgctgaat attttccgct gctttggcga
     3421 agcggaagta attgctgagc cgatgatcca cccggtggtc ggtgtgagcg gttcttcgcc
     3481 agcctacgtc tttatgttta tcgaagcgat ggccgacgcc gccgtgctgg gcgggatgcc
     3541 acgtgcccag gcgtataaat ttgccgctca ggcggtaatg ggttccgcaa aaatggtgct
     3601 ggaaacggga gaacatccag gggcactgaa agatatggtc tgctcaccgg gaggcaccac
     3661 cattgaagcg gtacgcgtac tggaagagaa aggcttccgt gccgcagtga tcgaagcgat
     3721 gacgaagtgt atggaaaaat cagaaaaact cagcaaatcc tgatgacttt cgccggacgt
     3781 caggccgcca cttcggtgcg gttacgtccg gctttctttg ctttgtagag cgccaaatct
     3841 gccgatttca accactcacg atagtgactc atttgtgggt tcagcggcgc aacccccaca
     3901 ctaatccgta acatcacctg cggcgtattc ggcaggcgta acgtatttaa tccttcatgc
     3961 actcgtaaca tggcggtaat ggcgctctca gctggcgtac cggacatgat taccgcaaac
     4021 tcatcgccgc caaaccgacc aatcacatcg ctaccgcgca gggtaatttg taactgtcgg
     4081 gtaagcgcca caatcgcttc atcgcccaca tcatggcccc aggtatcgtt gatgctcttg
     4141 aaatggtcga tatcgataat cagtaacgtt gcatcgcgat tatgccgccg acagttatca
     4201 aattcattgc gtaacatagt ttcccaatga cgtcggttat acacgcccgt catgccgtcg
     4261 cgggtactca tgacctgcaa cctgcgttta tgttccgcca gtttaatcgc agtctggtag
     4321 ctgacccagc caaacagcag aggataaatg acaataatgg gaagggagag ccaccattcc
     4381 agcggcgcac tattgaacga cacggtgatg cccgtcagct cgagggtgac aaggcaggaa
     4441 accaccatca acaccagacc cgcgacaaac agacgggggc cgcctgcccc catcaaattc
     4501 agacacataa tcatcaacat cgcggtggaa ggcagcacgt ttacgcccat tacgcctacc
     4561 cacattcccg ctaatactgc atcggttttt aagttgtaaa tttcccggct aagcggatcg
     4621 acggccctgc tcgctatctg ccaggctaaa tgcggccaga cgaacgccca gccgaccaac
     4681 accagccacc accagcccgg cggcgggtgt gaaaccagcg ttgaagcaat cggtaagaac
     4741 atgccagcca ggccaaccgc acggggtagt ctgacgcgac gggcgaagcg cagcccggac
     4801 cgctgatgtt cgttttgagg agttaaagga ggttcctccc cgtgcgccgc cgcttttttg
     4861 aaaaagtttt catcattcat tatttttggg aacattcaga aacaattttc ccaaattata
     4921 gagacggggc taaaggcaga agtatgaaat ttcgggtgag ccattttagt agctcacccg
     4981 ttagcactat caggtatcag gcggctttct tgaggcaggc gctcataaac ttattacgat
     5041 catcaccttt cagtgattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct
     5101 gtggcgtcaa acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt
     5161 aggtcttacg agcatcccct ttcaacgcct gtgccgtcgc ctgctgatta caggaggtca
     5221 tacgctgttg ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca
     5281 gaccgaaaag caaggtaacc agtaatgtaa ttttcatagc accatccctc ttcatgtttt
     5341 aaccatgagc gtatgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac
     5401 cacccggcag cgaaaattca ctgccgggag cggttttatt tcagccccag agcggctttc
     5461 atggtgtaga agagatcggt ctggtcggtc agtccaacga cattggcggc atgcgggcca
     5521 tacgccgcaa tacgcaactg actgccggta tgttcttgtg aatcctcttc ggagttcccg
     5581 taactcatca ccatcactgc gccatctttg gtatttagcg cctgggtgag gcccggagct
     5641 ttggtatccg gcgcaacaat ctggctggcg tgggcgtgat cagcggtgac tatgaccagc
     5701 gtgttaccat cctttttggc gaattccagc gcccgttgta cggcttcatc gagatcgacc
     5761 gtctcgccaa tttgcccaca aggattcgca gcatgatcct gtttatcgat tgacgcacct
     5821 tcaacttgca ggaaaaagcc tttctcattt ttactcaaca attcaatggc tttgtcggtc
     5881 atctgtgcca gggtcggtac actgtcatta cgttgcggat taggcgtaca ggtgactgcg
     5941 ggcttatcga tattaccgtg gtacgttgct ttcggtccta gccagcgcac tggcatattg
     6001 ccgtcagcaa acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt
     6061 gaggcagcat cgctcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt
     6121 ccctgccatt caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta
     6181 acgtcggcac gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga
     6241 gcgttacccg gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaggtcaca
     6301 tgtgccacca gcgcggcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc
     6361 gccagacctg cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg
     6421 acgcccagcg cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc
     6481 gagtcggtga cgtagtccgg tttgccggtt tttttattca gcgcatagtg agtgtattgc
     6541 ccggtaagcg gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta
     6601 cgtgcggcag taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt
     6661 gcaggtttat cgctaagaga atcacgcaga gcggcggtct gatcccccgt taaacggcga
     6721 gcaccgccgg gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct
     6781 ggtgtccggg cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg
     6841 ctttgtttca ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact
     6901 ataagtctcg gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgacg
     6961 aggatgcttc aataacttct ttacgtaatc gcgcagcagc tccgtatcgt cgtcaggaat
     7021 gctggcatcg ggctttaccg cgtacagcgc cccctctacc tgatcaatca accgttgttg
     7081 gtcattttgc gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg
     7141 cgcacacagt tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat
     7201 gagatttttc atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca
     7261 caacttcata gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat
     7321 tttttgtttg attaccagcg aaacgtttc
//
LOCUS       DAZFFG010000184         7282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFFG010000184 DAZFFG010000000
VERSION     DAZFFG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.185"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..992)
                     /gene="pyrD"
                     /locus_tag="ACHJ7J_004640"
     CDS             complement(<1..992)
                     /gene="pyrD"
                     /locus_tag="ACHJ7J_004640"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO5002759.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIK"
     gene            complement(1103..1840)
                     /locus_tag="ACHJ7J_004641"
     CDS             complement(1103..1840)
                     /locus_tag="ACHJ7J_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5002760.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1806..2321)
                     /locus_tag="ACHJ7J_004642"
     CDS             complement(1806..2321)
                     /locus_tag="ACHJ7J_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002761.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTLNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDVKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2329..2871)
                     /locus_tag="ACHJ7J_004643"
     CDS             complement(2329..2871)
                     /locus_tag="ACHJ7J_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002762.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKFQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2883..3953)
                     /locus_tag="ACHJ7J_004644"
     CDS             complement(2883..3953)
                     /locus_tag="ACHJ7J_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002763.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTTGVVKDKFYQWNESNDYYVSCDCDKDNVRNGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARINVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3944..6544)
                     /locus_tag="ACHJ7J_004645"
     CDS             complement(3944..6544)
                     /locus_tag="ACHJ7J_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO5002764.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACALLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGT
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGISWNY
                     SKSRGQPDADQVFALNFSLPLNLLLSRSNDSYTSKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTTKQGIPLRF
                     GAIATLDGVQANSGIIDDDGSLYMAGLPAKGTITVRWGEAPDQICHISYELTEQQINS
                     AITRMDAICR"
     gene            complement(6569..7270)
                     /locus_tag="ACHJ7J_004646"
     CDS             complement(6569..7270)
                     /locus_tag="ACHJ7J_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5002765.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         1844 a         1861 c         1626 g         1951 t
ORIGIN      
        1 tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac cagtgaggca
       61 cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac accgatgatc
      121 ggtaagcgac cgtttaattc cagtgacaag cggcgaataa tttcggtgct ttttaactga
      181 agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg aaccagagaa
      241 cgatcgagtg tggtattggt tgcaatcacg ccatcaatat tatggcgaac taaactatcg
      301 gcaacctgga tcaattcttc ttcagaaaga tccggcgcga tcttcactgc gatcggcaca
      361 tatttatggt gcatcgcttg caaatcattt tgtttatttt taatcgcagt taagagatca
      421 tccagcgctt caccatattg cagtgtgcgt aatcctgggg tatttggcga tgaaatattg
      481 atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata gtcatctttg
      541 ccctgctcta ctggcgtatc tttatttttg ccgatgttaa tacccaggac gccgtcataa
      601 tgggcttttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa gcccatacgg
      661 ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt acctggctgt
      721 ggacgtggcg tgacggtacc gatctctatc gatccaaatc ccatcgcgcc taacgcatca
      781 atgcactccc cgtctttgtc aagaccggct gcaagaccaa gcggattttt aaacgtcagg
      841 cccatgcagt taacaggttt tgcaggcact ttctgccgca ccagtgcttc aaacggtgtt
      901 ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg atcgagctgg
      961 aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc ggtgtgcaaa
     1021 ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg caaaaaaaac
     1081 gcaaacgttt tccgaacctg tttcagggca gattttgctc cttctttgca ctcaacccgc
     1141 cgtaatcatt tatcgcccgc caggcaacat gacacggtgc tattgcctga caccagcttg
     1201 tctgacgctg gcttttaggc ggcacaactc ccacattact taacgcttta ccatttacgc
     1261 tcacctgaat caggttaatg taataaggcg ttgggttagt gagttgtaca ccctgcgaat
     1321 tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa ccttcgggtc
     1381 gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt tcaactttgc
     1441 cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca ggcaagatgt
     1501 cgctttccgt tctcagcaag cgcaacgtgc ctgtcgtacc gggcttcagg agaataagtg
     1561 gcggagcggc cagtaatggt gctggcactg tcgacgcttc acccccggcc caacgcgttg
     1621 ggcggttgat tttactgtta ataagccagg attcctgact ggtattagtc agtaaaatag
     1681 atatcgattc tctgtctgcc ggaaagataa atcgcgttcc gccgaccacc actcctgcct
     1741 gcaattcacc cgccaccagc agcgataaaa cggcgaaaat caagagcgcc aatctgttcc
     1801 acgtattagt cataatgaag tacaaatgtc gcgctggcat tcactttacc cgactgcaca
     1861 tcgctattga caggtcgcaa ttgcgcataa aaagttaatg gcaccttacc gctatgtagg
     1921 ctgtagtctt tactcgcctg gttaagcgga atcaatatct tcacatcatc cagaatcgca
     1981 attgccagcc cgctggctgc atccgtacca ctttcaatgg acaacatttc gctctgcact
     2041 ccgtcggggg ttcctgaaaa ggtgacgtca accgtgctga catctgttcc gcaatcctgc
     2101 aaattaatga tgaaagattt ctccgggcca taattcccat gagaaaaacg tgtcagtgag
     2161 acatcgccta actgcacatt gatttgcttg ctatcctgtg ccagcacgca tgtattacgg
     2221 cgaaacgatc cgctaatgtt gagcgtgcca tcatgggcaa atagctcaca accccccgtt
     2281 aatcccagta agaacagaat ccagattatc cgtttcaaca tcactccttc actggtaaac
     2341 cagatcaaaa tagagaaccg ccgtcgcatt accgcccgtt gctcccgcct ttgtggactt
     2401 ataacgaagt tgaaatttga gtgtgttatc cccggctttt aagggcgtaa gaggctggac
     2461 ctgattgagc gggatttctt gctgactttg cgcatcaaga atttgcacgg caatccccgt
     2521 tgccatacca ccgcttccgc ctgtgtcagt tagtttgagc agtgtcggcg caagggtgtc
     2581 agcctcgccg ctaaaaagga cgtccgcgcc agtaatacca gcggcacaac cggtgatatc
     2641 gatattcaaa tcagcagcag tgcttacggt attcgcggcc tgaaaatcac tgatattaaa
     2701 atcaccaata tgaatatttt gtaatgcact tttggtggcg acatcacagc cctgatcgac
     2761 cacattccca taaatatgca tactaacttg ttcaggccca gccatcccga catggatgtt
     2821 aagaatgcta aagaacaaga taacgcactg aaatatcgtt tttttcttca tacgttcacg
     2881 cgttaatcaa aatttattct caccgttgcc gtggcttcaa atcgcccggt ttctggtggt
     2941 acacctgtcg tgctgactgg atagacattt attcgcgcga tactgtctgc atcatcagga
     3001 atagagagcg gtagtgttcc accagcaggg gaaataatgt tgttctggct atcggtcacc
     3061 accacgccca cgccaggacg tgacgtctta atagcctggg gatagctggg gtctgaggtt
     3121 gcagtcagac tcaggttgac gttaactaac cccgcgtttc ccgtgttaca cctgaccgga
     3181 attgcaagtt ctgccgttct ggccccaaga ggcttatgcc ctacaacacg aaagttagcc
     3241 gcaaatacgt cgcccagatc aacctcgatt gtttctccgg cattgacgga acatgagcca
     3301 agtgaattga tcgtaccaga caggtaatac actaaaaccg gtgtacctgt agtcgtgcag
     3361 gaatcacctg cgggtatgtt gtagcattca aataatcgcg ccagcgccat aggaggaatg
     3421 acgaccgagc caacgaacgg ctgcaatatt ttcagggaaa agctaccgct attaccgctt
     3481 gccccctgac cacctaaacg ttgaccgcca gggtcgcaaa tatgccatcg ggtatccccc
     3541 cctgtgccca cgttttcgaa aggaaccgct gtaggagaac tgcctttaac ctgcaataaa
     3601 accttggcgg caagatagtc attaattttg taccagttgt cgcctaaata gactaacggt
     3661 gaatctgctg cgaatgccca tcgaccattt ctgacattgt ctttatcgca atcacaggaa
     3721 acataataat cattcgattc gttccactga taaaatttgt ctttaactac cccagtcgta
     3781 ttttcttctt cctccagggt tttaacgcca aaattgacgc caatatacgg attcccctga
     3841 gtggtatgac actgcccgct ataagcagcg gtttgagcgc accatagcat taagacggcg
     3901 gcaaaaaata gtcgtagtaa tgacacgcat ttttctccaa agattatctg catatggcat
     3961 ccattcgcgt aatcgcagag ttaatttgtt gttcggtaag ctcgtaactg atatgacaaa
     4021 tttgatcggg agcttcgccc cagcgcacgg ttattgttcc cttcgccggt aaacccgcca
     4081 tatagagcga gccatcatca tcaattatgc cgctattagc ctgtacgccg tccagcgtcg
     4141 ctatcgcgcc aaaacgcagc ggtataccct gctttgttgt tttcattaat acccttgctc
     4201 ccgcatgggc attgaactcc gccagaacca acgccccttt ggtgggaacc acgttgacta
     4261 ctgcattttc aagatccacg ttacgtttta acgacgccgc atcaagtgcg attcgatttt
     4321 cacgataaga agtggcataa ggaacaacgg tatatccgcg ccagtcagtt ttcagcccgg
     4381 tgctgttcgc cacacgagta ttctctgcgc ctggtgctgc aatcagaacg ttagtttccc
     4441 ccagtgattg ccccagggta atgccctgtg aatgggcaac taaactgcct gaaagagcgt
     4501 agttcagttg ttgttgactg ccgttatcgc tgtagttgta gcccacccgg gcatccgcaa
     4561 acgccccttt gtagtccatg ctggcgctgc cattagccgt tttcccctcg ctgttatatc
     4621 cctgttgcac gctgtagctc aggttaccgt catcgagtag tgtctccgtt aaacccaggt
     4681 tttgtgtagt gtgcccttcg ttatcgatac tggtgttaga ggtcatccag gcgtaatttt
     4741 ttttgctggt atagctatca ttacttctgg agagcaacag attgagtggc agggaaaaat
     4801 taagtgcaaa cacctgatca gcatcaggtt ggccacggga cttactgtag ttccaggaga
     4861 tccccagcga gagatcttta atttgcgtgt tatagccaaa ctgtaatagc cgatcctgtt
     4921 gatcggtatg ccagtaagtt tgctggctac cacttaaata aaacgaaccg tactcgccta
     4981 attgctggga gatattgacc tgcagtttgc cacgtttggt gtaaaacaaa ttgtaataac
     5041 gcgaccacat cggcgtatct tcatcatcac cgtcattaaa ttcgtagcca tccatatgtt
     5101 tatacatggt gtcggaaagg gtgtagaaac ccgacgtcga atagcgatag cccatcaatt
     5161 gaaaattagt gcccaattgg ttgagcgttt tggcatacag aaaacgatat gactgacctt
     5221 tatgttcgct ttggtctgcc agcgtactct tcgcctgggt cgcatcgaac gaaattgctc
     5281 cgaaatcgcc gaggttaaat cccagaccaa acatggcggc acggtaatat tcagcatatt
     5341 gtccaccacc ataccaggtc gttccccacg ttccgcccca ctgcaacgtg gcctgggcaa
     5401 atttgctctc ctgctgttca ttactattgg ttctgtattt cgccagtgtc accgcgtatt
     5461 tgattcgccc ctgacgctgg agtaatggca cgctggaaaa gggtacgctg tagctattga
     5521 cgctgccgtc cgcttctttg atttcaacta acaaatcacc gctcgacgac gtggaataga
     5581 gatcactaat ttcaaaagca ccaggcgata cataagtttg gtaaatggtg taaccatttt
     5641 gcttaatcgt tatctgggca ttgcttttgg cgataccacg caccacaggc gcaaaccctt
     5701 tcaggctgtc tggcagcatg ttgtcatctg acgccagctg cacaccacga aagctgacac
     5761 tatcgaaaaa atcgcctgca gtataatcat cacctacggt cagttcgcct ttcagaggaa
     5821 taaccgcccg ttgcaaatag ctgctgagat tcttccattc tgcggtttgg ccactactac
     5881 gactccatgt tgaattgttg cgcaatctcc atgggcctaa atttacgcca ctgtttaaat
     5941 tcagaaaata actgtcgcca gaatcactgt ctgcgctgct atgaatactg ttagccccgc
     6001 taaatgaata tcccagtaat agcgcgttga tcccttcatc ccaacgttca ggggggacat
     6061 aaccacgagc attacttttg atggcaattt gcggtactga aatttccaga cggagtttat
     6121 tcacattaaa agttacgctg gcatccggaa taatctccgc caatagcgca cacgcttgtt
     6181 tgtccagttg tttaaaagcc ggaaatgcat cagtattcac gcccattgca tccaggcttt
     6241 cagtcgtcaa acaggcggca atccccttct ctggcgttat ctcaacaaaa ttgacgttgc
     6301 gcgtatctac aattgtctga ttcagaacaa tatcgacacg ataaacccct ggcgttattt
     6361 tttgtccttt ttcgaaacga gacaaatcgg ccaccattga gggatcatct ttgaaaaagg
     6421 ctggatcgaa ccaggtttca gcttgtgcat tgaacgctgc cgacacaaac accaccagcc
     6481 caccaataaa cgatggcacg ccaccagagc ttaacaggct gtgttgtcgg tgagttctat
     6541 acatctcgct ctcttaactg aagacgaatt acgatttaca gttgagttgc ggcgtttccg
     6601 ccccgtagtc attgatggtt cgatacgtta cgctgctatt tttggcaccc tttggcagag
     6661 agaattggta tttatcaaag ggggaaatca tttgattttt aactaccttt ccacctattt
     6721 tcaggtctgt cagggtaatg aaataaggtg ttgggttact gaccgtaacc atgtcagctg
     6781 acgaagtgca acgcagtttc gccggagcct cgcctaaggg atacgctaaa ccttttggtc
     6841 gccagaaaag tttgatgacg ctttgtgtgg caatcttcag ggtattgcct tcttcccgct
     6901 tcgttgttga agggactgcc cgtacgctag tgaagaaaag agtttctctg tctttcgcca
     6961 acggcgcgcc aatgtacata atgcgtaaca gattctcatt agccggattc agcacaaata
     7021 gcggtggtgt aataataaag tctgctgatt ttttattatc tggggtcatc acccatgact
     7081 gaataagata aggaacatcc ggactggtgt tacgtacacc cagtgaaatt tgctcttcac
     7141 cttctgaata aacaagacgt gttgctgcaa gtccaatccc gcctttacct gccgcccatg
     7201 cggatgagca gctgagtaat attgccgtta aacttacggt cacaattccc tttgttatgc
     7261 aagttttcat aaatagtcac tc
//
LOCUS       DAZFFG010000185         7266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFFG010000185 DAZFFG010000000
VERSION     DAZFFG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.186"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            24..230
                     /locus_tag="ACHJ7J_004647"
                     /pseudo
     CDS             24..230
                     /locus_tag="ACHJ7J_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(319..1455)
                     /gene="yibH"
                     /locus_tag="ACHJ7J_004648"
     CDS             complement(319..1455)
                     /gene="yibH"
                     /locus_tag="ACHJ7J_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5002766.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1458..1820)
                     /gene="yibI"
                     /locus_tag="ACHJ7J_004649"
     CDS             complement(1458..1820)
                     /gene="yibI"
                     /locus_tag="ACHJ7J_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5002767.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKVTDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2357..4270
                     /gene="mtlA"
                     /locus_tag="ACHJ7J_004650"
     CDS             2357..4270
                     /gene="mtlA"
                     /locus_tag="ACHJ7J_004650"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO5002768.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGHK"
     gene            4500..5648
                     /gene="mtlD"
                     /locus_tag="ACHJ7J_004651"
     CDS             4500..5648
                     /gene="mtlD"
                     /locus_tag="ACHJ7J_004651"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5002769.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5648..6235
                     /gene="mtlR"
                     /locus_tag="ACHJ7J_004652"
     CDS             5648..6235
                     /gene="mtlR"
                     /locus_tag="ACHJ7J_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO5002770.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6247..6456)
                     /gene="yibT"
                     /locus_tag="ACHJ7J_004653"
     CDS             complement(6247..6456)
                     /gene="yibT"
                     /locus_tag="ACHJ7J_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO5002771.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6741..7103
                     /gene="yibL"
                     /locus_tag="ACHJ7J_004654"
     CDS             6741..7103
                     /gene="yibL"
                     /locus_tag="ACHJ7J_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO5002772.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVDAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT         1842 a         1817 c         1839 g         1768 t
ORIGIN      
        1 tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc atcatcgcaa
       61 tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg aacaactcat
      121 atcatcattg cgatctcgac aatgtatagt ctgatccttc ggcagatcaa gacggataaa
      181 aaatccaatg agttcacact catcccgaac ttcttaacat aatggatatt aacgaaaaat
      241 catcacagcc gatgaaataa tatgcaaaaa taattagtcc ttacagggag caaagttcca
      301 taatctcccc cttccccctc aatgatccaa ataaagataa tgcatccagc tggtcattct
      361 tagcagcact tttcgcatca ccgaaacatg gctgaaatgg tcggagtaaa ctgccacctg
      421 ggcgtaaatg ccatcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc
      481 cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct gcgcctgata
      541 agaaccgcct ggcacgacag gtaaaatact ggtcagtttg ccgtggaaca cctgcccagg
      601 tagcgcgtta aacaccactt ccgcatcatc gccaggtttc agacgtaaca gcgagttttg
      661 ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aaaaccatca ccggacgcaa
      721 cggcaaggca gctgcgtatg tgcctgggcg gatcagtacc tgagtgacgt agccattgct
      781 tggtgcgcga atgacagtct gctcaagatt atattttgct tcagtaagtt gcgctcttaa
      841 gctcacaatc tgcgattgct cgccgttgac catactgtcg agctgactct ggatctgcgc
      901 ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt gccgcgcatc
      961 gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca ggtaacgttg
     1021 ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc
     1081 agtgagctgc gcgcgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag
     1141 tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg
     1201 aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct gtggcgtgat
     1261 ggggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa
     1321 caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca gcgtccactg
     1381 attcaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt aagttaaaac
     1441 aatcaataga tccataatta gatctccggg aacgtggaat aatcagtctt tttctcggcg
     1501 gcgagttggt gctccagcct ggaaattcgg tcagaaagtg cggcgatttc cgggtcagtc
     1561 actttctcct gcgacgcaac atgtgactgc atgccccagc cgcgctccgg ttgatagagc
     1621 gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct cacccagccc
     1681 gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt tggcaatcag ataagggatg
     1741 tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag cactcccaac
     1801 gcgaaatagt ttagaaacat atttgcctcg gattcacgtt tatcagtgtt gttttttggc
     1861 tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg cgattctcgc
     1921 cataacacca aagaataatt tttagaggtg atgagttgct tagttacata acgattgtat
     1981 gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa atatcttgtg
     2041 attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgatatta gtcacattta
     2101 aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg ttttgtgatg
     2161 aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc acacaaaagg
     2221 ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac aaggtccggc
     2281 tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga cagcaaacat
     2341 aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct ttggtcgttt
     2401 cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc
     2461 gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat
     2521 gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg taggcggcga
     2581 acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg cagacatgcc
     2641 gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta agcacttcga
     2701 ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata acttctccgc
     2761 aggcatcatc gggatgatcc tcgccattct ggcatttctc ggcattggcc cgattgttga
     2821 agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg acatgctgcc
     2881 gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg ccattaacca
     2941 cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat ccatattctt
     3001 cctgattgaa gctaacccag gtccgggtat gggcgtgctg ctggcgtaca tgttctttgg
     3061 tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatt atccacttcc tgggtggtat
     3121 ccacgaaatt tacttcccgt atgtgttgat gaatccgcgt ctgatcctgg ccgttatcct
     3181 cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg tttctccggc
     3241 atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt atttcgctaa
     3301 catcgcgggt gtgtgtgcgg cgatggctgt ctcctttgtt gtctctgcta ttttgctgaa
     3361 aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta tgcaggacat
     3421 gaaagctgag tctaaaggtg catctccgct gtctgcgggc gatgtgacta acgacctgag
     3481 ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc
     3541 aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtcg cagatttcgg tcactaacag
     3601 tgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg acctgaccga
     3661 acgcgctatg cgccaggttc cgcaggcaca gcatatttcg ctgaccaact tcctcgacag
     3721 cggcctgtac accagcctga ccgaacgtct ggttgctgcc cagcgccata ctgaaaacga
     3781 agtgaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta acctgttcaa
     3841 actgggcgcg gagaacatct tcctcggtcg caaagctgcc accaaagaag aagcgattcg
     3901 ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat
     3961 gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg tgccacacgg
     4021 tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga
     4081 aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt ctggtgattg gtattgctgc
     4141 ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg atgatgagtc
     4201 cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac tgctggcagg
     4261 tcataagtaa tccaatccca ccctctccac atggagaagg tggggctaat tgcctaatgc
     4321 gctacgctta tcaggcctac aggatgcatc acaatttgtt gaatttgcac gttcttgtag
     4381 gccggataag gcgtttacgc cgcatccggc gcagcccctc tccacatgga gagggtttgg
     4441 gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag gttaatacta
     4501 tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt aaactgctgg
     4561 cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt gatgccctga
     4621 atgcccgtca tagctatcag gttcatgtgg taggtgaaac cgagcaggta gataccgttt
     4681 ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt tgatctcatt gctcaggttg
     4741 atttagtcac taccgccgtt ggcccggtag tgctggaacg tattgctccg gctatcgcca
     4801 aagggctggt gaaacgtaaa gaacaaggta atgaatctcc gctgaacatc atcgcctgtg
     4861 aaaacatggt acgcggtacc acgcagctga aaggccatgt gatgaacgcc ctgccagaag
     4921 acgccaaagc gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt gaccgcatcg
     4981 taccgccttc ggcttcggca actaacgatc cgctggaagt gacggtagaa accttcagcg
     5041 aatggattgt cgataaaacg cagttcaaag gtgcactgcc gaatatccca ggcatggagt
     5101 taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac acgggtcatg
     5161 ctataaccgc gtacctcgga aaactggccg gtcatcagac cattcgtgac gctattctcg
     5221 acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggcgcg gtactgatca
     5281 agcgttacgg ctttgacgct gacaagcatg cggcgtacat ccagaaaatc ctcggtcgtt
     5341 ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg ctgcgtaaac
     5401 tgagcgcagg cgaccgtctg atcaagccac tgctcggtac gctggaatat ggtctgccac
     5461 ataaaaacct gattgaaggt attgccgctg caatgcactt ccgcagtgaa gatgatccac
     5521 aggctcagga actggcagca ctgatcgccg acaaaggtcc gcaggcggcg ctggcacaga
     5581 tttccgggct tgatgccaac agcgaggttg tatctgaggc ggtaaccgct tataaagcaa
     5641 tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat tgtcagatat
     5701 gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc gtctgaatgc
     5761 tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag ctcctgaccg aggcggtaaa
     5821 tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaaatatg ctgtagaacc
     5881 gttactcgac ggcgatggtc cgctgggcga tctttctgtg cgtttaaaac tcatttacgg
     5941 gttgggcgtc attaaccgcc aggaatacga agatgcggag ctgctgatgg cattgcgtga
     6001 agaactaaat cacgacggca acgagtacgc ctttaccgac gacgaaatcc ttggcccctt
     6061 tggtgaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac cagcagactc
     6121 cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa caatggtcct
     6181 ttcactgaca gagctgattt ccaaaatcag cttaaaaaaa gcctttcaaa agtaagcaac
     6241 gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat actccagacg
     6301 ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt tacggggagg
     6361 aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta cggctttatc
     6421 gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc
     6481 gccgggaatg cgcggccgcc aattttagtt taggttcccg gtaaagatga cattaaggaa
     6541 aacgtgctga atcctcaata atcatccgct tctgtttatt atcttttttc tatagatttc
     6601 ttaattaatc aataaattgc gtagttttta agcatattaa tgattacgtt gcggaactat
     6661 tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta
     6721 tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg
     6781 atcgtctgga cgccatccgc caccagcagg ccgatctgtc gctggttgat gccgcagaca
     6841 aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgcc cgcctgcgtg
     6901 aagttcacag ccagaaactg agtaaagaag cacagaagct gatgaagatg ccgtttcagc
     6961 gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac
     7021 tggtggttgt tcacccaatg accgcgctgg gccgcgaaat gggcctggaa gagatgactg
     7081 gattctctaa gactgcgttt taagcccctc agacgattgg tacgatcgta tcaacccgca
     7141 tctggcttgc gttagctggt caatgcgggg actcccctgt ctgctcgcct aattactgaa
     7201 caatccactc cctctacatc aatttaccgc tggttgatat tctgaaaata tatttttacc
     7261 gaaata
//
LOCUS       DAZFFG010000186         7130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFFG010000186 DAZFFG010000000
VERSION     DAZFFG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.187"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..589)
                     /locus_tag="ACHJ7J_004655"
     CDS             complement(<1..589)
                     /locus_tag="ACHJ7J_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5002773.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGR"
     gene            741..1931
                     /locus_tag="ACHJ7J_004656"
     CDS             741..1931
                     /locus_tag="ACHJ7J_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5002774.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTAMYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            2012..2662
                     /gene="inaA"
                     /locus_tag="ACHJ7J_004657"
     CDS             2012..2662
                     /gene="inaA"
                     /locus_tag="ACHJ7J_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO5002775.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(2716..2970)
                     /gene="yfaE"
                     /locus_tag="ACHJ7J_004658"
     CDS             complement(2716..2970)
                     /gene="yfaE"
                     /locus_tag="ACHJ7J_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO5002776.1"
                     /translation="MARVILRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(2970..4100)
                     /gene="nrdB"
                     /locus_tag="ACHJ7J_004659"
     CDS             complement(2970..4100)
                     /gene="nrdB"
                     /locus_tag="ACHJ7J_004659"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5002777.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(4334..6619)
                     /gene="nrdA"
                     /locus_tag="ACHJ7J_004660"
     CDS             complement(4334..6619)
                     /gene="nrdA"
                     /locus_tag="ACHJ7J_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5002778.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            6747..6890
                     /gene="ypaB"
                     /locus_tag="ACHJ7J_004661"
     CDS             6747..6890
                     /gene="ypaB"
                     /locus_tag="ACHJ7J_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO5002779.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         1674 a         1698 c         1873 g         1885 t
ORIGIN      
        1 agcgtccttt actaatgatt aatggttcac ccattaattc ttccactgta atagtgctgt
       61 tttttgccag agcatggttg ggcggtaaca ctacggtgaa tttatcctga taaatgggca
      121 cggagtacat tccgctcacc ggaaaatgaa caaaaccggc atcaatggat tcattctgaa
      181 gcgaatcaat aatcgctgtg ctattttcct caaaaggaat aatttttata ttcggatgat
      241 gaatttcaaa gtagcgaatc acaaccggta agatacaggc gcagacgctg gggaaacaac
      301 caagtctcag cgttctttga gggttctgct tttcctgctc ggctatctct tttactgcgt
      361 taacttcccg attaatggcg cgcagatgtt gcacaatgcg gtgacctgct gtggtcagtt
      421 gtatagcttt acggcgatcg cgtaacagta tattcacacc aagttcttgt tcgagggcag
      481 caattgcctg acttaccgcc gactgcgtca tgaacaatcg cttgctggct tcagtaaagc
      541 caccgtaatc cagcacggcg agtaaagaat ttatttgggt aagagtcatt agagtttgct
      601 aatcaataat attaaaacga ttaatttctc taatagcatg ccgccattta tacttctgtt
      661 ctgcaaagta catcacaatt tgacgcaacc gcagtaacgg gagtgttgtc tgcgtgcgtc
      721 atcctgacag gcggtagggt atgaaagaga agttatggac gaaggatttt tgggcaataa
      781 ccatcatcag ctttattatt ttcttcgtct tttatgtttt actaacattg ttgccaattt
      841 atatctctga ccgcttgcat gcctctcctg ataaagcagg tttgttggtg actttatttt
      901 taattgcggc gattgttatt cgaccctttg ccgggcaatg ggtgggtaaa tattcgaata
      961 aaactattct ggtgctctct tctctggcct ttttggtggt cactgcgatg tatccttttt
     1021 gccactcaat agaatcactg ctttttatta gggtgcttca tggtattacc ttcggggtta
     1081 tcacaacggt aaagggaacg atttccgcgc ggctgatccc ggcctcccga cgtggggagg
     1141 gcatcagttt tttctctctg gcaatggggc tggcaatggt ggtcgggccg tggattggcc
     1201 tgaatatggc gcgctgggag gcctttaata tggctttctg gttatgtact ggcgttgcgg
     1261 cggtggggat tatcctgtcg ctgattatga ccgtgccgcc ggttatcagc catgccgacg
     1321 gttcaaagcc aaagatgggc ttcgccgcca tgttcgatcg cgcggcattg ccgtttgcca
     1381 tggttacatt ctttatgacc ttttcgtatg ccggggtttc tgcctttctg gcgctttacg
     1441 cccgcgaact taatctgatg tcggcggcca gtaatttcct gctctgctac gccatcttcc
     1501 tgatgatctg ccgtaccttc accggcaatg tttgcgacaa aaaaggcccg aaatatgtgg
     1561 tttacccctg cctgctgttc tttacggttg ggctggtggt tctcggctac acccagggca
     1621 gcgtaatgat ggtcgtttct ggcgcgttga ttggtatcgg gtatggttcc gtgacgccag
     1681 tttttcagac gcagattatc agttcagtgg aaccgcataa aatcggtgtc gcaaactccc
     1741 tcttcttcaa tgcgatggat gcaggcctgg cgctgggagc ctgtgtgatg gggatgatgg
     1801 ttgcacatac tggctaccga atgatttatc tgctgggcgc actattagtg gtagtggctg
     1861 gtggagtcta tgcgctgcaa atgaagggta aaagcggtgt cgcgctagta gtggcaaaag
     1921 aaattcatta atacgacacg tttcattaag atttttcgca ggtaaccacc tatagtcatt
     1981 gtcgttaata aaaaatgaca ggcgataggg tatggcagtt tcagcaaagc acgacgaatt
     2041 taatcactgg tgggcaacag agggcgactg ggttgaagag cctaactatc gccgtaatgg
     2101 tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag ctatatgtaa agcgcatgac
     2161 gcatcatctg tttcattccg tacgttatcc tttcggccga ccaacgattg tccgtgaggt
     2221 tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg ccgaagatcg tttttggtga
     2281 agcggtgaaa attgaggggg aatggcgcgc gttgctggtg actgaagata tggcggggtt
     2341 catcagcatt gctgactggt atgcccagca tgcagtaacg ccctattctg acgaagtacg
     2401 gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa atgcatagcg ttaatcgtca
     2461 gcatggctgt tgttatgttc gccatatcta tgtgaaaaca gaaggtaagg cagaagctgg
     2521 ttttcttgat ctggagaaaa gtcgtcgccg cttgcgtcgc gataaagcga ttaatcatga
     2581 cttccgccag ctggaaaaat atctggagcc gatccccaaa gcggactggg agcaggtaaa
     2641 agcgtattac tacgccatgt aatcgttgta ggccagataa tacgcgtaag cgtcgtatct
     2701 ggcgctagta cccgatcaca actcgatttc aatatcgcct tttgcccggc aacaacaggg
     2761 caaaatttcc cccggctgaa taaaggctaa cggttcggca atccagtcca cctggcctgc
     2821 aacaaggcgc gtgcgacagg agccgcagta accttcgcgg cactggtact caaccgccac
     2881 attgtgggat tccagcgccg ccagaaggga agggtgttca tcctggcaca gcagttgtgt
     2941 gccagtgatg cgcagaataa cgcgggccat cagagctgga agttactcaa atcgtcggtg
     3001 tccacttccg agtcaatctg cccgaccaga taagaactca cctccacttc ctgtggagca
     3061 acctgcacgt tatcagacac cagccaggtg ttgatccacg ggatcgggtt ggaacgcgtc
     3121 tggaacggca gatccaaacc aaccgcctgc atacggatat tggtgatgta ctccacgtac
     3181 tggcagagaa tgtctttgtt cagaccaatc atcgaaccgt cacggaacag ataatccgcc
     3241 cagtctttct cctgttgtgc cgcctggaca aacaggtcat agcactcctg cttacattct
     3301 tcggcaattt ccgccatctc cggatcatcc gcgccgctgc gcagcagatt cagcatatgc
     3361 tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca ggcgaataat tttggcgtta
     3421 ccttccatca gctcacgttc agcgaatgcg aaagagcaag caaagctgac gtagaaacga
     3481 atcgcttcca gcgcgttaac gctcatcagg cagagataca gttttttctt caactcgcgc
     3541 agactaacgg ttacggtttt accgttaacg gtgtgggtgc cttcgcccag caggtgccag
     3601 tagctggtca tttcgatcag ctcatcgtaa tagctggaga tcccttccgc acgtttctgg
     3661 atctgctcgt tggtgacgat atcgtcaaac acaacagacg gatcgttaac gatattacga
     3721 atgatatgag tataggaacg ggaatgaatc gtttctgaga acgcccaggt ttctacccag
     3781 gtttccagtt ccggaataga aataagcggc aatagcgcca cgttcgggct acgaccctga
     3841 atggagtcca gcagcgtctg atatttcagg ttgctgataa agatgtgttt ttcgtgttca
     3901 ggcagcgcct ggtaatcgat acggtcacga gaaacgtcaa cttcttccgg acgccagaag
     3961 aaagagagct gcttttcgat cagcttttcg aagatgtcat atttttgctg atcgtagcga
     4021 gccacgttga ccggctgacc aaaaaacatc ggttctttga gctgatcatt tttcgtctgt
     4081 gaaaaggtgg tatatgccat gagtgtgtcc tgttgggagt ttaatgccgg ataaggcatt
     4141 ttacgccgca tcctgtgcct gatgcgacgc tggcgcgtct tatcaggcct acaaatccga
     4201 gccgtaggcc ggataaggcg ttcacgctgc atccggcacc cggagcctga ggcgacgctg
     4261 gcgcatctta tcaggcctac aaaccgagcc gtaggccgga taaggcgttt acgccgcatc
     4321 cggcatctca atatcagatc ttacatgcgc cgctttcgca gccatcgtcc tggattgacg
     4381 gcaccagatc gtcctgcgcg tcttcagcgc cgtcacgggt gttctgataa tacagcgttt
     4441 tgaccccgaa tttgtaggcg gtgagcaggt ctttcagcaa ttgctgcatc ggcacttttc
     4501 ctgacgggaa gcgtgacgga tcgtagttgg tgttagcaga gatcgactga tcgataaatt
     4561 tctgcatgat acccaccagt tgcaggtagc catcgttacc cggcatttcc cacagcagct
     4621 cataggcgtc gtgcagatgc tcgtagtccg gcaccacctg gcgcagaata ccgtctttcg
     4681 acgctttgat gctgacgtaa ccgcgcggcg gttcaatacc gttagtggcg ttagagatct
     4741 gcgaagaagt ctcggacggc atcagagcag aaagcgtgga gttacgcagg ccgtgcgttt
     4801 tgattgactc acgcagagct tcccagtcgt aatgcagcgg ctcattagcg atggtatcca
     4861 gatctttctt ataggtatcg atcggcagga tccctttcgc gtaagtggtt tcgttaaacc
     4921 acgggcacgc gccttgctct ttcgccagct cattagacgc tttcagcagg taatactgaa
     4981 tcgcttcgaa ggttttatgc gtcaggttgt tggcgctgcc gtcggagtaa cgtttaccgt
     5041 gcttcgccag gtagtaagcg aagttgatca ctccaatacc cagcgtacga cgacccatcg
     5101 ccccgcgttt ggcggctggg atcgggtaat cctgataatc cagcagcgcg tcaagtgcac
     5161 gaaccgccag aattgccagc tcttccagtt catccaggct attaattgcg cccaggttga
     5221 aagcagacag cgtacacagc gcgatttcac cgttctcgtc gttgacgtcg ttcagcggtt
     5281 tggtcggcag ggcgatttcc aggcacaggt tagactggcg cactggcgcg atggccggat
     5341 caaacgggct atgggtattg cagtggtcaa cgttctgaat atagatacga ccggtagatg
     5401 cacgttcctg catcatcagc gagaacagct caacggcttt cacacgctgc ttgcggatgc
     5461 tgtcgtcttt ctcatatttg gtatacagac gttcaaactc ttcctgatcg gcgaagaacg
     5521 cgtcgtacag ccccggtacg tcggacgggc tgaacagggt gatatcttca cctttcagca
     5581 gacgggtata catcagtttg ttgatttgta ccccgtagtc catatgacgc acgcggttgc
     5641 cttccacacc acggttgttt ttcaacacca gcaggctttc cacttccaga tgccacatcg
     5701 ggtagaacag cgttgccgca ccgccgcgca caccgccctg agagcaggat ttcaccgctg
     5761 tctggaaatg tttgtagaac ggaatacaac cggtatggaa cgcttcgcca ccgcgaatcg
     5821 ggctacccag cgcacgaata cgcccggcgt tgatgccgat cccggcacgc tgggatacgt
     5881 atttaacaat cgcgctggag gtggcgttga tggagtccag gctgtcgccg cactcgatca
     5941 gtacgcagga gctgaactga cgagtcgggg tacgcacgcc ggacatgatt ggcgtcggca
     6001 gcgaaatttt aaatgtggaa accgcgtcgt aaaaacgctt cacgtattgc aggcgcgttt
     6061 cacgcgggta gttcgagaac aggcacgcag caaccagaat ataaaggaac tgggcgcttt
     6121 cgtagatttc gccggtcacg cggttctgta ccagatattt gccttccagt tgcttaacgg
     6181 cagcataaga gaaggtcata tcacggtcgt gatcgataaa cgtgtccatc tgcttgaact
     6241 cttcttccgt gtagtcttcc agcagatgat tatcgtattt gcccatctcg accattttca
     6301 ccacgtggtc gtacagcgca ggcggctcaa actggccgta ggctttttta cgcaggtgga
     6361 agatcgccag gcgcgcggcg agatactgat aatccggcgc atcacgggag atcaggtctg
     6421 cggcagcctt gataatagtt tcgtggatgt cagaggtctt gataccgtca taaaactgaa
     6481 tgtgggagcg cagctcgacc tgggaaatcg aaacgttatg cagtccttct gccgcccaat
     6541 ccagaacgcg atggattttg tcgagattga tgcgctctgt gctaccgtcg cgctttgtca
     6601 ccagcagatt ctgattcatg tatgtcgtac ctgtttttgg aattctttcc gcaatagttt
     6661 catgcagact acctgtagta cgggaagctg cttagaacac tatatatagg gggtatgttt
     6721 gggattcact gcaagatagt gtgaaaatgg ccctcttgca agtgcataac tttgtggata
     6781 actcaggaag gaaaaagtgg ctttcgcgca ccttaggtca gacaaggtgt ccgggaaagt
     6841 caatgggaag agaaaaattt gttaaaaata actgttcggc gatttcttga acggcagaat
     6901 aggttggata aattgatata gatggtttat taaccttttt gctaatgatt gcaaaaattg
     6961 atctaagcac gagtcatcac ataatctgat gtatcagttg tggtcaatat ataagcgaat
     7021 gtaagcgatt gttgatgtcg aaatggttgt tttctattcg gttattgtta aatgtgaagc
     7081 cattttgtta atttacctta tattataaga aaattattca ttactttaat
//
LOCUS       DAZFFG010000187         7065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFFG010000187 DAZFFG010000000
VERSION     DAZFFG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.188"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(170..655)
                     /gene="sixA"
                     /locus_tag="ACHJ7J_004662"
     CDS             complement(170..655)
                     /gene="sixA"
                     /locus_tag="ACHJ7J_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO5002780.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(858..3002)
                     /gene="fadJ"
                     /locus_tag="ACHJ7J_004663"
     CDS             complement(858..3002)
                     /gene="fadJ"
                     /locus_tag="ACHJ7J_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO5002781.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENIELKQQMVSEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVARMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3002..4312)
                     /gene="fadI"
                     /locus_tag="ACHJ7J_004664"
     CDS             complement(3002..4312)
                     /gene="fadI"
                     /locus_tag="ACHJ7J_004664"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO5002782.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4493..4777)
                     /locus_tag="ACHJ7J_004665"
     CDS             complement(4493..4777)
                     /locus_tag="ACHJ7J_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO5002783.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5149..6489
                     /gene="fadL"
                     /locus_tag="ACHJ7J_004666"
     CDS             5149..6489
                     /gene="fadL"
                     /locus_tag="ACHJ7J_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO5002784.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6854..>7065
                     /locus_tag="ACHJ7J_004667"
     CDS             6854..>7065
                     /locus_tag="ACHJ7J_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002785.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEVNGLVQEFKSLFNGKEGIS
                     TCIKHLLELIKNAIRVNDNPDRFNIN"
BASE COUNT         1807 a         1874 c         1646 g         1738 t
ORIGIN      
        1 tacaacttag tctcatttcg tcagaaatta atagggaagc agaggaaaaa gtgcgatgaa
       61 ttctgtatcg aagcgtatca actccgtgaa aggttttgag tgaatatcta ctattgcaaa
      121 aagacaaatg cccgatgtac agcttacatc gggcattttt tcaaacgaat cagatagctt
      181 tcgccatctt caaattacat gggctcatct gccagttgaa ctcgcctttt ccgctttcat
      241 caagcgttac gctggcaatg gccgaggtgg taaacatagg cggcgtttcg cccggacata
      301 actcggcaac cagatacccg actaacggta agtgggagat aaccaacacc gaagcaacac
      361 cttcattggt caacgcctgc aaataggcac tgaccagacc aacatcgccg cagggcgtta
      421 actccggcag aacttctgca ctggaaggca ggttcagaca atcccctact tcctccagtg
      481 tttgctcggc tcgcaggaac gggctcacca gaacgcgttc gatttccact ttttgacctt
      541 tcagccagtt cgccatcagg cgagattcgt cacaaccatt agtagtcaga ggacgaaccg
      601 aatcactggc ggcatcgagg gctgcgtcgc cgtgacgcat gataaaaact tgcatattgc
      661 accgcttttg ttaaccagat tcgcccgttt tctttcatct gaaaccagag aaagaaaacg
      721 gtggccggca ttgtgcctta tccattcacc gaatgaaacg ctgtttttta cctcaatggc
      781 gtaagtatag tcaatatgcg tttacatttt gaccaacaat ccgccattca gcgcggattc
      841 atatagcttt gaccttctta ttgcaggtca gttgcagttg ttttccaaaa actttcccca
      901 cgcgcaccca tctcgactaa acgctcgcaa ggggtaaaac gtgaaccata ctgcgtggcc
      961 agtcgttgca ttcttgcaac cacttcgccc gcgccgagag aatcgatata gcggaacggt
     1021 ccaccgagaa atggcggaaa accaatgcca aataccgcgc caatatcccc gtcacgcacg
     1081 ctacggataa cctgctcatc aacacaacgt actgcttcat tcagcatcaa catcacacac
     1141 cgttcagcaa cctgcggtgc ggagagtcgc ccctgccctt gtgcgccaat gagcgggtaa
     1201 atggcaggat cgacctgttt tttgctttta cgccctttct gaccataaag atagaaaccc
     1261 cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg aagaaacaac atttgcaggc
     1321 gcgctaaaac gttctccata agcggcttcc agtacaggaa taattttagt cccggtgtcg
     1381 attcctacct catccaaaag ttggattgga cctaccggaa aaccaaattt cactagcgcg
     1441 gcatcaatgt gctctacccg ctcaccttcg gtcagcatgc ggatagcttc attaatgtaa
     1501 ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac gcacgacaat gggcgttttg
     1561 ccctgttttt tcgccagttt taccgtagtg gcgatggttt gcgctgatgt acccgcatga
     1621 ggaataatct ccaccagcgg cattttttcc accggactga agaaatgcaa gccgataact
     1681 tgctcaggtc gcgtggcgtg agcggcgata tcaccaatcg gtaaagatga cgtattcgaa
     1741 gcaaagatgg tatgagcggc gcaattttgc tcaacttccg acaccatctg ttgtttcaat
     1801 tcgatatttt caaacaccgc ttcaataatc agatcgcgat gggcaaagcc gcgatagtcc
     1861 gtcgttccgg agattaatgc cagctgtttg tcacgttcgc tggctttgag atgacgacgg
     1921 cgaactttgc cctccagctg atcccaactg tacttcagcg catgatttat gccctgcggg
     1981 ttgatatcct taattctgac cggaagcccc gctttacaag cagtgacata agcaataccg
     2041 ccgcccatca agccaccacc taaaattccc acgctgttta atggcgcagg cggcgcatca
     2101 ctaccgggat ctttcttcac ttccgtactg gcaaaaaaga tactacgcaa agcttgcgat
     2161 tgtggcgtca tcgccagttc gccaaacgcc cgtgcttcag cgtcatagcc actgctggtg
     2221 ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt ctgtcgccgg ataattgcct
     2281 tgagttttgt gttctgtttt cttgccgacc attttgaaca gcagcgcacg acctaacggc
     2341 cccgccagaa tacgctcgcg tacaggtaga gggcgggaag atgggcgatc cttctttgcc
     2401 agctcaacag cggcttccag cagaatggag tgcggaacaa cgtcatccac cagccccagc
     2461 tttaatgcct gtttcgcccg aagttgtttt ccggtgagga tcatctctaa tgctgtgctg
     2521 acgcctatca gacgcggtaa acgctgggtg ccgcctgaac cgggtaacaa tccaagttgt
     2581 acttcaggca aaccgagcac cgttttagga tcgtcagtac aaacgcgacc gtggcacgcc
     2641 agcgccaact ccagcccacc acccaggcaa gcgccatgaa tagccgcgat aaccggaatg
     2701 ggcaaagcat gaatctccgc catcaactgt tggccctgcc gcgccagagc ttccgcttct
     2761 tgcgccgttt tgcagttgcc gatcatgttg atgtcagctc cggcgataaa gttgtctggt
     2821 ttcgcggaaa taaataccac gccgcgcaac tctttgtttt cgcggagttg cttaataatg
     2881 gcgcgcacct gcgaggcaaa ttccgccttc agggtattca ttttctcacc cggtacgtcg
     2941 atggtgataa cggcaatgtt gtccagacga acattaaggg taaacgctga tgtcatttcc
     3001 attattccgc ctccagaacc attgccgcgc caagcccacc ggcagcacag gcggtcacta
     3061 aaccaaatcc accgccgcga cggcgaagtt catgcaatgt ctgggtaatc atccgcgcgc
     3121 cggtcgccgc gaagggatgc ccgtaagcta tcgaaccgcc aagcacgtta aatttgctat
     3181 cgtccacttc gccagtggca tgtgcacgcc ccagtgcttc acgagcaaaa cgttcactac
     3241 ccagcaactg aatattcgcc agtgtctgag ctgcaaaggc ttcgtgcata tcgatcaatg
     3301 tcagatcgct catcgtcaaa ccggcacgtt ccagcgccag cggtgttgac caggccggac
     3361 cgagcaacat gtcctgccag acatcgatag cggtaaatgc gtagctgcgc agatatccca
     3421 gcggtaccag ccctaattct ttcgcccggg attcagtcat caggatcacc gccgctgcgc
     3481 catcggtcag cggcgtactg ttcgccgccg ttaccgttcc gtgtttacga tcaaacgccg
     3541 gacgcagctt tgcgtaatca gcaagcgagg aattaccgcg aatattgttg tcttccgcga
     3601 gcagttgttt ataaggaggg ataaaggcag tcatcacctc ttctttgagt tttccgtctg
     3661 accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa tgcatcttgc tgttctcggg
     3721 tgatgccgta ggttttcgcc atttgctctg cggtgtcgcc catccgcaag ccggtagaat
     3781 attctgctac cgcaggtggt acgggcatta agtcgcgcaa acgcaggcga gagaagagtt
     3841 tcagtcgctg gctcatagtt cgagctttgt tgacatcaac cagcacgcgc gccagttttt
     3901 tactgacgcc aattggcaat accgaagagg aatctgcccc accggcaatc cccgctcgga
     3961 tagtccccgc catcaggctt tctgcgacgt ttgcaaccgc ctggaaactg gtagcgcaag
     4021 cgcggctgac gctgtaagca tcggtatgca cattcattcc agtaccgaga acaatttcac
     4081 gcgcaatgtt gggagcttca ggcatttgta cgacctgacc aaagaccagt tgttcaatca
     4141 cttcggcggg gatctcgctg cgtgccagca gttcgcctac caccatcttc cctaaatcaa
     4201 ccgcgggaat gccatgaaaa gccgtcgcct ggcgggcaaa aggcgtacgt aaaccgctaa
     4261 caatggcgat acgatcgccc tggcgggtaa ccagcggtaa aacctgaccc ataacactcc
     4321 cctgtaaaaa aataaataaa gtggtctgac ctgatcatag tcttaaccat ttttttacat
     4381 ttagccaagc ggagaaaagg gaaagtggga gctatgacac agagagaaaa gaagaagaga
     4441 aaagaaaacg ctcctgccag caattgacag gggcgtacgt acccgagagg aattaacgca
     4501 gacccagctg gaagatcagc atttcagctt cgcaggcaaa agtgaaatcg atatccaggc
     4561 gcacaccatc agactcttca gtgaaagtcg gggagatttt gcacggttcg gattccacgc
     4621 tacgggcttt ctcagtcagc gccgccagcg tttgttctgc ttctgcgcgg tttgcaaaca
     4681 cgcggctgta agacgcggtg cagtcggagt tgtccataat ggtgccaaca tccatacagc
     4741 agcaaaccgg ggtttcatca gcactacatt tactcatcgt tgatttcctc tgtatgtgca
     4801 cccaaggtgc cagataaacg ttgtggatat tttacgcttc cggaaagtgc tgctccagtt
     4861 gttaattctg caaaatcgga taagtgaccg aaatcacact taaaaatgat ctaaaacaaa
     4921 attcacccga atccatgagt gcgccacctc caaattttgc cagctggatc gcgtttctta
     4981 gatcatattt gaaaaaagat agaaacatac ttgcaacatt ccagctggtc cgacctatac
     5041 tctcgccact ggtctgattt ctaagatgta cctcagaccc tacacttcgc gctcctgtta
     5101 cagcacgtaa catagtttgt ataaaaataa atcattgagg ttatggtcat gagccagaaa
     5161 accctgttta caaagtctgc tctcgcagtc gcagtggcac ttatctccac ccaggcctgg
     5221 tcggcaggct ttcagttaaa cgaattttct tcctctggcc tgggccgggc ttattcaggg
     5281 gaaggtgcaa ttgccgatga tgcaggtaac gttagccgta accccgcatt gattaccatg
     5341 tttgaccgcc cgacattttc tgcgggtgcg gtttatattg acccggatgt aaatatcagc
     5401 ggaacgtctc catctggtcg tagcctgaaa gccgataaca tcgcgcctac ggcatgggtt
     5461 ccgaacatgc actttgttgc accgattaac gaccaatttg gttggggcgc ttctattacc
     5521 tctaactatg gcctggcaac agagtttaac gatacttatg caggcggctc tgtcgggggt
     5581 acaaccgacc ttgaaaccat gaacctgaac ttaagcggtg cgtatcgctt aaataatgca
     5641 tggagctttg gtcttggttt caacgccgtc tacgctcgcg cgaaaattga acgtttcgca
     5701 ggcgatctgg ggcagctggt tgccggtcag attatgcaat ctcctgccgg gaagactcct
     5761 caagggcaag cattggcagc taccgccaac ggtatcgaca gtaataccaa aatcgctcat
     5821 ctgaacggta accagtgggg ctttggctgg aacgccggta tcctgtatga actggataaa
     5881 aataaccgct atgcactgac ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac
     5941 agcagcgatc ttaatcgtgc gtttaataac tacggcttgc caattcctac tgcgacaggt
     6001 ggcgcaacgc agtcaggtta tctgacgctg aacctgcctg aaatgtggga agtgtcgggt
     6061 tataaccgtg ttgatccgca gtgggcaatt cactatagcc ttgcttatac cagttggagt
     6121 cagttccagc agctgaaagc gacctcaacg agtggcgaca cactgttcca gaaacatgaa
     6181 ggctttaaag atgcttaccg catcgcgttg ggtaccactt attactacga tgataactgg
     6241 accttccgta ccggtatcgc ctttgatgac agcccagttc cggcacagaa tcgttctatc
     6301 tccattccgg accaggaccg tttctggctg agtgcaggta cgacttacgc gtttaataaa
     6361 gatgcttcag tcgacgttgg tgtttcttat atgcacggtc agagcgtgaa aattaacgaa
     6421 ggcccatacc agttcgagtc tgaaggtaaa gcctggctgt tcggtactaa ctttaactac
     6481 gcgttctgat aacgcgttcg cctggataaa gtcacctgct atgcaggtga ctttaactcc
     6541 cccacttcac cgaaagtagt ttcctcattg cttgcctcct gagttttgca aacagcctga
     6601 tggcagctag ccctttcaaa tcagtaagtg gtatccacag acaaacctga agggaaacgc
     6661 atattttcag gcgttctgct cgtccttctc aaagagttta cttttctgaa tttccaggat
     6721 actcaacccc ctggctattg tgcgcttata cacgcaaatt aaagataggt tctaaataaa
     6781 tgagcgtttt ttgatagtct attttattag gtaatatata tttgtaacaa atcaatcaac
     6841 atggaataaa atcatgctac catctatttc aatcaacaat accagcgcag cttacccaga
     6901 atccatcaat gaaaataaca atgatgaagt taatggatta gtacaagagt tcaaaagcct
     6961 ttttaatggt aaggaaggaa taagcacctg tattaaacat ctgcttgagc ttataaaaaa
     7021 cgccatacga gtaaacgaca atcctgatag atttaatatt aataa
//
LOCUS       DAZFFG010000188         6972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFFG010000188 DAZFFG010000000
VERSION     DAZFFG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.189"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..116
                     /locus_tag="ACHJ7J_004668"
     CDS             <1..116
                     /locus_tag="ACHJ7J_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002786.1"
                     /translation="FNKFEIACDSDGFYIFKALIQLRRFTGFKNVRLYQLH"
     gene            159..719
                     /locus_tag="ACHJ7J_004669"
     CDS             159..719
                     /locus_tag="ACHJ7J_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002787.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            805..1506
                     /locus_tag="ACHJ7J_004670"
     CDS             805..1506
                     /locus_tag="ACHJ7J_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5002788.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGDNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKHNR
                     IEVTNPTGYYVVIRAAELLNNGKKVPLANSVMIAPQSTTEWTLRSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1559..4081
                     /locus_tag="ACHJ7J_004671"
     CDS             1559..4081
                     /locus_tag="ACHJ7J_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5002789.1"
                     /translation="MNKSVVSISAAILVLLCQPVMGKEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAQELAPGITSQTQLSQLRLDLSVPQSQMISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GTVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDLTWQHGMTNTLTFNSG
                     SRIADGYQALMLGGVYGSTLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLTNYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSNDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGVTFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDSDAELVDSEKQVAP
                     VAGAAVKVVFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            4097..5431
                     /locus_tag="ACHJ7J_004672"
     CDS             4097..5431
                     /locus_tag="ACHJ7J_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002790.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDGSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYIFNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5428..6117
                     /locus_tag="ACHJ7J_004673"
     CDS             5428..6117
                     /locus_tag="ACHJ7J_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5002791.1"
                     /translation="MKSVLLLLTVLFVQSVEAGIMPASSRMIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPEEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGIVTATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6263..>6972)
                     /locus_tag="ACHJ7J_004674"
     CDS             complement(6263..>6972)
                     /locus_tag="ACHJ7J_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5002792.1"
                     /translation="AGELGRGLCSALAKAGANLLLVDIKEPDNRYLKHLTHEGVEVEF
                     MTIDITKPDAACTIINRCLERFGQLDILVNNAGVCNINRPIDFNRNDWDPMINLNLNA
                     AFDMSQAALNIFVPQRKGKIINMCSVLSFHGGRWSPGYAATKHALAGLTKAYADDFAE
                     YNIQINGIAPGYYVSEMTAIIYNNPKIKELIKGRIPAQRWGRAQDLMGAMVFLASAAS
                     DYVNGQLLVIDGGYSIR"
BASE COUNT         1860 a         1706 c         1640 g         1766 t
ORIGIN      
        1 tttttaacaa gtttgaaatt gcttgtgata gtgatggttt ttacattttc aaggcactta
       61 tacagttaag gagatttacc ggttttaaaa acgtcagatt gtatcaattg cattaattaa
      121 ctcgttctat attaactcta aatttcaagg atattattat gttttctact ttcaaaaaag
      181 cagctctgct ggcagctatt gcattacctt tttcaactat ggctgcgcct acagtcactt
      241 ttcagggtga agtaaccgat cagacctgtt ccgttaatat caacggtcaa accaattcag
      301 tagtattgat gccgaccgta gccatggctg acttcggtgc aactttagct gatggtcaga
      361 gcgcaggcca gacgccgttt acggtttctg tgtctaactg ccaggctcca actggtgcag
      421 atcaggcaat taacaccacc ttcctgggct acgacgttga cgctagcacg ggtgttatgg
      481 gaaaccgtga taccagcagc gatgcggcga aaggctttgg cattcagtta atggattcca
      541 gcacttctgg taacccagta actctggctg gcgcgactaa cgtaccgggt ctgaccctga
      601 aagttggcga tactgaagcc agctacgact tcggtgcgcg ttacttcgtt atcgatagcg
      661 cagctgccac tgccggtaaa attaccgctg tcgcagaata caccctgagc tacctctaat
      721 taatctactg gctggcagta aatgtcagcc agtatttctt tgcacaatta atagtgattt
      781 ttcatggaca tggaaaagat aagaatgaac atttttgcat atttactggt acttgtattt
      841 tccatgagca tgagcagcag cgcgtttgcc agcgtggtaa tgaccggaac ccgtattatt
      901 ttccctggtg acgcaaagga aaaaaccatc cagttgcgca ataccagcga tcagccctat
      961 atcattaata tccatgttga ggatgaacgt ggttctgaca agaatgtacc gtttatgcca
     1021 accccgcaga catttcgcat ggaagctgcc gctggtcagg cgttacgcct gctctacact
     1081 ggtgataatt taccgcagga tcgcgagtct gttttctggt ttagtttcag tcaactacct
     1141 tatctgaata agaacgacaa aagtcagaac cagctcatcc tggccctgac taatcgagtc
     1201 aaaattttct atcgtcccag ctcgattgtc ggtaaatcca gtgacgcgcc aaaaaacctg
     1261 acttatcagg taaaacataa ccgcatagaa gtgacgaatc ccacaggcta ttacgtcgtg
     1321 attcgcgccg ctgaactgct taataacggt aaaaaagtcc ccctcgcgaa ttccgtcatg
     1381 attgcacctc aaagcacaac tgaatggaca ctacgctctg gcatcagcgt cgctcccggt
     1441 gcgcagattc atttagtgac cgtcaatgac tatggcgtaa atgtcacgtc tgagcatgcc
     1501 ttataagctc tttccccttt tttgagcggt atcctttttc acgcacgagt atgtcgagat
     1561 gaataaatcc gttgtgtcaa tttctgcggc aatattggtt ttactctgcc agccggttat
     1621 ggggaaagaa atctcacccg ctacaccgtc agatgaagac aactacacct ttgacccgca
     1681 actctttcgc ggcagcagat ttagtcagtc gtcattggca aaactgacaa ctcgtgagtc
     1741 tgttgcaccg ggcaattata aaatggatat ctacaccaat aataagttgt caggcagttg
     1801 gaatgtcacg ttcaaagaag ccgctgatgg tcgcgttctg ccctgcctga cgcctgaagt
     1861 cgcggacgcg ataggcctca aaacagggga agataagggt gaaaaagatc cggtctgtac
     1921 ctttgctcag gaactcgctc ctggcatcac cagccagaca cagttgtcac aactacgcct
     1981 tgacttgtcg gtgcctcaga gtcaaatgat tagtcgccct cgcggctatg tccctcccag
     2041 cgaacttgat accggagcat cgctggcgtt catgaattat attgccaact attacaacgt
     2101 tgcctattca gggcagaatg ctcatagcca gcgttcgcta tgggcatcat ttaatggcgg
     2161 catcaacctt ggtgcctggc aatatcgtca gttatccaac atgacctggg ataatgacaa
     2221 agggaatcag tggaacaata ttcgtagcta tttgcaacgc ccgctgcccg ccataaatag
     2281 ccagttaatg atggggcagc ttatcaccag cggaagattt ttctctggac tcagttatca
     2341 cggcgttagt ctcgcgaccg atgaacgtat gctgccggac tccatgcgcg gctatgcgcc
     2401 gactattcgc ggcgtggccg caacaaacgc cagagtctcg gtaatgcaaa acggtcatga
     2461 aatatatcag accaccgtgg ctcctggccc tttcgagata aacgacctat accccaccag
     2521 ttacagcggc gatctggatg tcaccgttac ggaagcaaat ggcacagtca gtcgtttcag
     2581 tgtccccttt tcagccgtac cagaatcgat gcgtccagga acttcccgtt ataacgtgga
     2641 agtaggtaaa acgcaggata gcggtgatga ctcgatgttt ggtgacctca cctggcagca
     2701 cgggatgact aatacgctga catttaacag tggctcgcgt atcgctgatg gctaccaggc
     2761 gctgatgctt ggcggagtct atggcagtac gctgggggca tttggggcaa acctcacttg
     2821 gtcccatgcg cgtgttcccg aaagcgaagc acagagtggt tggatgtcgc aattaacctg
     2881 gagtaaaact ttccagccca cgtcaaccac tgtctctctg gcaggttatc gatactctac
     2941 cagcggctat cgtgatctgg ctgatgtgct gggagagcgt catgctgcca gcaataaaca
     3001 gtcatgggac tccagccaat ggcgtcaaca gtcgcgcttc gatcttacgt taagtcagag
     3061 ccttacgaat tacggcaacc tgtttgtgtc aggttcaaca cagaactacc gtggcggtaa
     3121 gagccgtgat acacagcttc agttaggtta cagcaatagc tttagccatg gcatcagtat
     3181 gaacctttcc gtcggacgcc aaagaatggg cggctataaa gacaattcta atgatatgca
     3241 gacggtaacg tccctttcat tctcattccc acttggcggc aatggacctc gtgtaccaag
     3301 tcttagcaac agctggaccc attcaactga cggcagctcg caattacaaa gctcgctaac
     3361 cggaatgctt gatgaagcac agaccaccaa ctacagcctg aacgtcatgc gcgatcaaca
     3421 atataagcag acgacgctta gcggaaacat gcaaaaacgt ttttcacaaa ctaccgtcgg
     3481 attgaacgca tcgaagggcc aggattactg gcaggcttcc ggaaacgtac aaggtgcgat
     3541 ggccgtacat ggtggcggcg ttactttcgg accttatctg ggtgaaacgt tcgccctggt
     3601 cgaagctaaa ggcgctgaag gtgcaaaagt ctataactcc agtcagctgg aaattaatga
     3661 cagtggttat gcgcttgttc cagcagtaac gccctatcgc tacaaccgta tatctcttga
     3721 tccacaagga atggatagcg atgccgagct ggtcgacagt gaaaaacagg tagcaccggt
     3781 tgcaggtgcg gcggtgaaag tagttttccg tacccgtcct ggtaaagcgt tgctgattaa
     3841 atcccgcatg gcagatggtt cggaattgcc aatgggagcc gatgtgctgg atgagaataa
     3901 tacagtcgtc ggtatagccg gtcagggggg gcaaatttac ctccgcacag aacagacaaa
     3961 aggccatttg tcagttcgct ggggtgaagg cgctaacgat agctgccaat tgccctttga
     4021 tatcagcggg aaggacagca atagccctat catccgcctg aatgaaacct gtcagtcttg
     4081 atacagagaa aacccgatgt ctaaatcgac ttttttacac attctgatta gctccatcat
     4141 attggtggca ctaatacaat catccgcctg ggctaactgt acaaacacac agataggtca
     4201 aactgaagat ggtcggacag cgcttattga gttcggaaaa attaatatga ccgacaccta
     4261 ttttgcgcca gcaggttcac tcctcgcgac aacggtcgta cctcccacta actacacatc
     4321 aggcggcgcg acaggaagct ccgtattgtg ggaatgtgat gcaacagatt tgccaaacat
     4381 ctattttctg gtcgctacca atggcgacga ccgcgtgggg ggcttttatg atgcaggcgg
     4441 gcctgatggt ttgagtgatg tctatgccac ctggttcgct tttgtcggtc tcaagcagac
     4501 catggcgggc gtgacgcttg gtcgttactg gaagaaagtg cccatcacca gttatgccac
     4561 tcagggaact aaaatccaga tccgcttaca ggatatccct cctcttcatg ctgagcttta
     4621 tcgcatcagt acgctacctg atacatcagc aacaacaagt tggtgcggta ataataatac
     4681 ggatggtagt ggagtcggat tcgcaaaacc ttccggtaca atctataact gtgttcagcc
     4741 caatgcctat attcagcttt ccggtaccag cggcatttta tttggtcatg atgagcccgg
     4801 tgaagatagt tctgttcatt gggatttctg gggggctgat aatggttttg gttacggaat
     4861 gcgttcggcc aatcgactct acaacaatgc cacctgcgtt gcccgcagcg ccacgccgtt
     4921 agtattgctg ccgacaattg cagaagcaca actgaatgcg ggcatggaaa gtaccggtaa
     4981 ttttaatgtc cgcgtcgagt gcagtaactc agttcaatca gggattagcg atactcagac
     5041 agcattagga atccaggtgt ctgaaggtgc atatacagcg gcgcaaaaac tggggattat
     5101 caatagcaac ggcggcgtca gcgccctggt ctctgataat tatgacgcag cagagatggc
     5161 aaagggcgtt gggatctaca tttttaacag tgctcacccc gatacggcga tgacgctggt
     5221 tggtcaaccg ggcatcgcga agttaacccc cggaggaaat gcagcggggt ggtatcctgt
     5281 atttgaaggg gcaacattag aaggtgcgac tcaccccgga tactccagct atagttactc
     5341 ttttatcgcc cggttgaaga aactgccaaa tcagacagtc agtgcgggaa aagtgcgggc
     5401 aacggcgtat atattggtga aaatgcaatg aaatccgttt tgctgctctt aaccgtcttg
     5461 ttcgtacagt cagtggaagc cggcatcatg ccggcttctt cacggatgat ttatcgcgat
     5521 ggcgatcggg aaaagacact gatgctggtc aataccaatg actacccggt actggtgcaa
     5581 agttgggtag ataatggtga aggttcccca gaagctgctt ctcccccctt tgtggtgctg
     5641 ccaccggttt tccgcctggc tgcgagacga acacagggat tgcggataat ctataatcat
     5701 gacccacttc cccaggatcg cgaatctgca ttctggttaa atctttatga aatgccgcca
     5761 gaaactcccc atcaagatcc cacacattta acgctggcaa tgaatacgca acttaaaatc
     5821 ttttacaggc ctaaatctgt aactatgacg cctgaagaag ctatgcgtaa aataagttgg
     5881 catctgggta acgacggcag cagtagctat attgagctta ataacccaac accactgaat
     5941 atctctttca catccatcag cattggggct acgaaggtac agcaagaaag cgatatgatg
     6001 ataaaaccct tcagtcaacg acggtactgg ttagcaagta ataacgttag tgggatcgtt
     6061 actgcgacat atctcgatga taatggtgca cagataaagc aacaattagc gcaataatgc
     6121 tctgagtggt atgtaaactc atcgcgatgg ttcaacgcta acgaataacc actatacccg
     6181 cctgaagtac cggaagtgga gaaatgacaa aatctctcca ctccattttc cagtcaaaac
     6241 acctcaacgc agccaaaaat cgttaacgaa ttgaatatcc accatcaatg actaatagtt
     6301 gtccattgac ataatctgaa gcggcactgg cgaggaacac cattgcgccc ataagatcct
     6361 gtgcccttcc ccagcgttga gccggtatac gcccttttat taactctttt attttagggt
     6421 tattataaat aatagcggtc atttcactaa cataatatcc tggggcgata ccattaatct
     6481 gaatattata ttccgcaaaa tcatcggcat acgctttggt cagccccgct aaagcatgtt
     6541 tagtcgccgc ataccctggc gaccaacgtc ccccatgaaa tgacaatacg gaacacatat
     6601 taataatttt cccttttctt tgcgggacaa atatattcaa tgcggcctgg ctcatatcga
     6661 atgcagcatt caggttgaga ttaatcatag gatcccagtc gtttcgattg aaatcgatag
     6721 gacgattgat gttacacacg cccgcgttat tgaccagaat atctaactga ccgaaacgct
     6781 ccagacacct attaataatg gtgcatgctg catcaggttt tgttatgtct attgtcataa
     6841 actcaacttc aacgccttca tgagttaaat gctttaaata tctattatca ggctctttga
     6901 tgtctaccaa caacagatta gcccctgctt tggccagagc cgagcataag cctctcccca
     6961 gttcacccgc gg
//
LOCUS       DAZFFG010000189         6952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFFG010000189 DAZFFG010000000
VERSION     DAZFFG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.190"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            21..299
                     /gene="yafQ"
                     /locus_tag="ACHJ7J_004675"
     CDS             21..299
                     /gene="yafQ"
                     /locus_tag="ACHJ7J_004675"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO5002793.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLRFERTGTHAALFG"
     gene            455..1195
                     /gene="dpaA"
                     /locus_tag="ACHJ7J_004676"
     CDS             455..1195
                     /gene="dpaA"
                     /locus_tag="ACHJ7J_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO5002794.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1166..1933)
                     /gene="yafJ"
                     /locus_tag="ACHJ7J_004677"
     CDS             complement(1166..1933)
                     /gene="yafJ"
                     /locus_tag="ACHJ7J_004677"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO5002795.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2139..2717)
                     /gene="lpcA"
                     /locus_tag="ACHJ7J_004678"
     CDS             complement(2139..2717)
                     /gene="lpcA"
                     /locus_tag="ACHJ7J_004678"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO5002796.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2957..5401
                     /gene="fadE"
                     /locus_tag="ACHJ7J_004679"
     CDS             2957..5401
                     /gene="fadE"
                     /locus_tag="ACHJ7J_004679"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO5002797.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5444..5890)
                     /gene="ivy"
                     /locus_tag="ACHJ7J_004680"
     CDS             complement(5444..5890)
                     /gene="ivy"
                     /locus_tag="ACHJ7J_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO5002798.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6071..6841
                     /gene="yafV"
                     /locus_tag="ACHJ7J_004681"
     CDS             6071..6841
                     /gene="yafV"
                     /locus_tag="ACHJ7J_004681"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO5002799.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT         1641 a         1751 c         1870 g         1690 t
ORIGIN      
        1 gataaattag gaatttaaat atgattcaaa gggatattga atactcggga caattttcaa
       61 aggatgtaaa acttgcacaa aagcgtcata aggatatgaa taaattgaaa tatcttatga
      121 cgcttcttat caataatact ttaccgcttc cagctgttta taaagaccac ccgctgcaag
      181 gttcatggaa aggttatcgc gatgctcatg tcgaaccgga ctggctcctg atttacaaac
      241 ttaccgataa acttttacga tttgagagaa ctggaactca cgcggcgctc tttgggtaaa
      301 gcccgctgcc gatgaaattt gtaaaccctg cgccgacagg cttgcgtaaa actcttttgg
      361 tgataatgtg agccttccaa atccagacta ttccgatttg ggatccattt tgccgtcctg
      421 gcgtgtgtaa ccgttttatc aaggaataag cagtatgcgt aaaatcgcat taattcttgc
      481 gatgttgttg atcccgtgcg tttcgtttgc cggtttgctg ggcagcagta gctcgaccac
      541 gccagtgagc aaagagtata agcagcagtt gatgggatcc cctgtctaca tccagatctt
      601 caaggaagaa cgtacgctcg atctctacgt caaaatgggc gagcaatatc aactgctcga
      661 cagctataaa atctgtaaat attccggcgg cttagggcca aaacagcgtc agggcgattt
      721 caaaagcccg gaagggtttt atagcgtcca gcgtaatcag ttaaaaccag acagccgtta
      781 ctacaaagcg attaatattg gtttccccaa tgcctatgac cgtgcgcatg gttatgaagg
      841 gaaatacctg atgatccacg gcgattgtgt ttccatcggc tgctacgcaa tgaccaatca
      901 gggtattgat gagatcttcc agttcgttac tggcgcgctg gtatttggtc agccgagcgt
      961 gcaggtgagc atttacccgt tccgcatgac cgacgctaat atgaagcgcc acaaatattc
     1021 caactttaag gacttctggg agcaactgaa gccaggctac gactactttg agcaaacccg
     1081 taagccaccg accgtttctg tggtcaatgg ccgttacgtg gtcagcaagc cgttaagcca
     1141 cgaagtggtg cagccacaac tggcatcaaa ctacacgctc cccgaggcaa aataagcgcc
     1201 attcgcctgg cataatcttt tgccaggttt cattgcccgt cagcggctgt gtcgcaatca
     1261 ccgtgaccac atcattcggt gtggtctgcg agctgaagtc gatttctaca tcctgatcca
     1321 gcaacgttgc cacgccaaac ggcgcgcggc gggtgatcca gtgtaaatta gtcgagcaat
     1381 acgccattac atagcgaccg tccgaaagca gcatgttgaa aacgcccttc gcccgcagtt
     1441 catccgccag tgaggcgata tatttaaaca ctgccgccat gttgcccggc gtgcgcgggt
     1501 aacgctgcgt taatttatgc agcagccagc aaaaggcttt ttcgctgtcg gtttcaccga
     1561 ccgggcggaa gttgccggtt tccagtgatt tgtagcccgt cagttgcccg ttatgggcat
     1621 aagtccagtt acgcccccat aactcgcggg taaacgggtg agtattttcc agcgccacct
     1681 cgccccgatt agcctggcga atatgagcca ccaccgaaca ggattttatc gggtagtcct
     1741 ggaccagttt ggcgataggt gagttaaagc tgggttgcgg atctttaaat gtgcgacagc
     1801 ctttaccttc gtaaaaggta atgccccagc catctttatg tggcccggtt ccaccaccac
     1861 gctgcaccag accggtgaaa ctaaagcaga tatcggtagg gacgttggcg ctcatcccga
     1921 gcaattcgca catgggtaac ctccacaaca gcgggagcgc atggcccccg gcgaagattg
     1981 atttaagaat tttcaggtcg gatgcggcgt gaacgcatta ttcggcctac ggttcgcgta
     2041 cagatttgta ggcatgataa gacgcgtcag cgtcgcatca ggcatcagcg cacaaatgcc
     2101 ggatgcggcg taaacgtctt atccggccta cgccagactt acttaaccat ctctttttca
     2161 atcaactgga tcaggatatg gatcacttta atgtgaatct cctgaatgcg gtcggcataa
     2221 ccaaagtgcg gtacgcgaat ttcgatatcc gccgtgccag ccattttgcc gccgtcttta
     2281 ccggtcaggg tgatcacttt cattcccttc tcacgcgccg ctgcgatcgc tttgatcacg
     2341 tttgcagagt taccggaggt ggagatcccc agcagtacat cgccttcgcg acctaccgct
     2401 tcaacgtaac gggagaaaat atcattgaaa ccgaaatcgt taccgacgca ggaaatatga
     2461 ctaacgtcag aaatagcaat cgccgggtag cccggacggt tttcacggta gcgaccggtc
     2521 aactcttcgg caaagtgcat agcgtcgcaa tgggaaccgc cgttgccgca ggaaagcact
     2581 ttgccaccgg ctttaaagct gtctgctaac aggaccgccg cgcgctgaat ggcgtgaata
     2641 ttggcgtcat cttttaaaaa gttagccagc gtttccgccg cttcgttcag ttcgttacga
     2701 ataagatcct ggtacatgag gatatccttc agcataaatg taatagacaa aatgcagtgt
     2761 accggatacc gccaaaagcg agaagtacgg gcaggtgcta tgaccaggac tttttgacct
     2821 gaagtgcgga taaaaacagc aacaatgtga gctttgttgt aattatattg taaacgcatt
     2881 gctaaatgtt tttacatcca ctacaaccat atcatcacaa gtggtcagac ctcctacaag
     2941 taaggggctt ttcgttatga tgattttgag tattctcgct acggttgtcc tgctcggcgc
     3001 gttgttctat caccgcgtga gcttatttat cagcagtctg attttgctcg cctggacagc
     3061 cgccctcggc gttgctggtc tgtggtcggc gtgggtactg gtgcctctgg ccattatcct
     3121 cgtgccattt aactttgcgc ctatgcgtaa gtcgatgatt tccgcgccgg tatttcgcgg
     3181 tttccgtaag gtgatgccgc cgatgtcgcg cactgagaaa gaagcgattg atgcgggcac
     3241 cacctggtgg gagggcgact tgttccaggg caagccggac tggaaaaagc tgcataacta
     3301 tccgcagccg cgcctgaccg cagaagagca agcgtttctc gacggtccgg tagaagaagc
     3361 ctgccggatg gcgaatgatt tccagatcac ccatgagctg gctgatttgc cgccggagtt
     3421 gtgggcgtac cttaaagagc atcgtttctt cgcgatgatt atcaaaaaag agtacggcgg
     3481 gctggagttc tcggcttatg cccagtctcg cgtgctgcaa aaactctccg gcgtgagcgg
     3541 gatcctggcg attaccgtcg gcgtgccgaa ctcattaggc ccgggcgagc tgctgcaaca
     3601 ttacggcact gacgagcaga aagatcacta tctgccgcgt ctggcgcgtg gtcaggaaat
     3661 cccttgcttt gcactaacca gcccggaagc gggttccgat gcgggcgcga ttccggatac
     3721 cgggattgtc tgcatgggtg aatggcaggg ccagcaggtg ctgggcatgc gactgacctg
     3781 gaacaaacgc tacattacgc tggcaccgat tgcgaccgtg cttgggctgg cgtttaaact
     3841 ctccgacccg gaaaagctgc tgggtggcgc agaagattta ggtattacct gtgcgctgat
     3901 cccgaccacc acgccgggcg tggaaattgg tcgtcgccac ttcccgctga acgtaccgtt
     3961 ccagaacgga ccgacgcgcg gtaaagatgt cttcgtgccg atcgattaca tcatcggcgg
     4021 gccgaaaatg gccgggcaag gctggcggat gctggtggag tgcctctcgg taggccgcgg
     4081 catcacactg ccttccaact caaccggcgg cgtgaaatcg gtagcgctgg caaccggcgc
     4141 gtatgctcac attcgccgtc agttcaaaat ctctatcggt aagatggaag ggattgaaga
     4201 gccgctggcg cgtattgccg gtaatgccta cgtgatggat gctgcggcat cgctgattac
     4261 ctacggcatt atgctcggcg aaaaacctgc cgtgctgtcg gctatcgtta agtatcactg
     4321 tactcaccgc gggcagcagt cgattattga tgcgatggat atcactggtg gcaaaggcat
     4381 tatgctcggg caaagcaact tcctggcgcg tgcttaccag ggcgcaccga ttgccatcac
     4441 cgttgaaggg gctaacattc tgacccgcag catgatgatc ttcggacaag gagcgattcg
     4501 ttgccatccg tacgtgctgg aagagatgga agcggcgaag aacaatgacg ttaacgcgtt
     4561 cgataaactg ctgttcaaac atatcggtca cgtcggtagc aacaaagttc gcagcttctg
     4621 gctgggcctg acgcgcggtt taaccagcag cacgccaacc ggcgatgcca ctaaacgcta
     4681 ctatcagcat cttaaccgcc ttagcgccaa cctcgccctg ctttctgatg tctcgatggc
     4741 ggtgctgggt ggcagcctga aacgtcgtga gcgtatctcg gcacgtctgg gggatatttt
     4801 aagccagctt tacctcgcct ctgccgttct gaagcgttat gacgacgaag gccgtaatga
     4861 agccgacctg ccgctggtgc actggggcgt acaggatgcg ctgtatcagg cagaacaggc
     4921 gatggacgat ttactgcaaa acttcccgaa ccgcgtggtt gccgggctgc tgaatgtggt
     4981 gatcttcccg accggacgtc attatctggc accgtctgac aagctggatc ataaggtggc
     5041 gaagatttta caagtgccga acgccacccg ttcacgtatt ggtcgcggtc agtacctgac
     5101 gccgagcgag cataacccgg ttggtttact ggaagaggcg ctggtggatg tgattgccgc
     5161 cgacccaatt catcagcgga tctgtaaaga gctgggtaaa aatctgccgt ttacccgtct
     5221 ggatgaactg gcgcacaacg cgctggcgaa ggggctgatt gataaagatg aagccgctat
     5281 tctggtgaaa gctgaagaaa gccgtctgcg cagtattaac gttgatgact ttgatccgga
     5341 agagctggcg acgaagccgg taaagttgcc ggagaaagtg cggaaagttg aagccgcgta
     5401 attgaataac ggagccgaaa ggctccgttt ctttatccgc taattattta aaattaaagc
     5461 catccggatg gttttccagg ctgccggtca acgccgcgaa cagcaccgtt ttaccatcaa
     5521 tcgaaagcgc atcgttcaca ttcaaccagg tgagtttctc ttgcgacgtt ttctcatcaa
     5581 tagtcgagaa cagccccgtc atctgattag atttctcgga ccacatcaca gcgatacgtt
     5641 gcgagccaca gtcatgcggt ttgcacgcgc tcatcacctg atacgtctca tctcccaatg
     5701 tcacggtttg tgcaggggta taagtaccgc ctttcatcac ccaggcaggc agcttatgcc
     5761 cttgcaccat ctgattaaat gcagctttgg tggtttcgcc ctttgcaagg ctgctaatgg
     5821 ttaaatcatc ctgcgccatt gcactggtgg cgatgaccag tgcggcgact gtcgttattg
     5881 ccttaaacat cattcctccc gagcttatcc tgcccatgta attaacctcc taaagcctgg
     5941 cgataacacg ataatttgca aatcagatat ttccaatatc agcatttgtt atcaatttat
     6001 tctgcgctaa ggcaactgcc tgataaggtg ttaaagttat aaaacccttc gcataaagga
     6061 gttgtacacc gtgcctggtt tgaagattac gcttttgcag caaccactgg tgtggatgga
     6121 tggtcctgcc aacctgcgtc attttgatcg tcaactggaa ggtattaccg ggcgcgatgt
     6181 gatcgttcta ccggagatgt ttaccagcgg ctttgccatg gaagcggcag cttcgtcgct
     6241 agcgcaagat gacgtagtga actggatgac agctaaagcg cagcagtgca atgcgctgat
     6301 tgcaggcagt gttgcattac aaacggagtc tggttcggtt aaccgctttt tgctggttga
     6361 gccgggcggc acggtacatt tttatgacaa gcgtcatctg ttccgaatgg cagatgagca
     6421 tctgcattat aaagcgggca atgagcgagt gattgtggaa tggcgcggct ggcgtatttt
     6481 gccgctggtg tgctacgact tacgttttcc ggtgtggtcg cgcaatctca acgattacga
     6541 cctcgccctg tacgtcgcca actggcctgc tccgcgctct ctgcactggc aggcattgct
     6601 gacggcccgc gcgattgaga atcaggcgta tgtggcggga tgtaatcgcg tcggcagcga
     6661 tggcaacggc tgccattatc gcggtgacag ccgggtgatt aatccacagg gcgagattat
     6721 cgctactgcc gacgcgcatc aggcaacgcg cattgatgcg gagctctcga tgatggcatt
     6781 gcgggaatat agagaaaagt ttccggcatg gcaggatgcg gatgagttta ggttacggtg
     6841 agtaggtgct gtgtttaggg cggggaattc agaaagggat gtttagtatg ctgagcgaga
     6901 gttaaataat aaaaagctgg gaagatttaa taatcatccc ggtttttatt tt
//
LOCUS       DAZFFG010000190         6841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFFG010000190 DAZFFG010000000
VERSION     DAZFFG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.191"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(3..596)
                     /locus_tag="ACHJ7J_004682"
     CDS             complement(3..596)
                     /locus_tag="ACHJ7J_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5002800.1"
                     /translation="MRFIDIPCHIISDYLYIRLRDADALCITDRKNSFFRKKAYGTFL
                     NSLMSLVEQQKNINLYESALFVLLDYLNDQKQLLPVLSCGLNNYSLRVEAIVAADPAK
                     KWYLSDVASVLCISASQLKRKLHSEGAGFSRIITEVRMKKAITLMRCGNGNIFVVARA
                     CGYNSLSYFIHSFKKYHSVTPCQWLKQYGIKDTTGDG"
     gene            complement(894..1523)
                     /locus_tag="ACHJ7J_004683"
     CDS             complement(894..1523)
                     /locus_tag="ACHJ7J_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO5002801.1"
                     /translation="MKHIFNAVAIALSLTSAVAMASATSENTSSTKNEAQTVLSERSL
                     KAEQRGMEIMDDMQYARLALFSGDTGTADRLINRAEDLLKSEDKTWETVVRKDKKTPQ
                     EGDVYIIINSSLMLSEDFTVTPEKQKAIDEANKKLDKGDKKGAIEVLHLAGIDISETQ
                     WLMPLKQTQKKVNSAQKLMSEGKYYEANLALKGAEDGVITDTVSLNEGN"
     gene            2044..2469
                     /locus_tag="ACHJ7J_004684"
     CDS             2044..2469
                     /locus_tag="ACHJ7J_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5002802.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQPGSSVARIAREHDIND
                     NLLFKWFRLWQNEGRVARRLPVTTSSDAGIELLSVEITPDEQKEPMATLPPLLSTPSQ
                     SPASASSCKGEFRHGKMTLENPSPELLTVLIRELSGRGR"
     gene            2466..2813
                     /gene="tnpB"
                     /locus_tag="ACHJ7J_004685"
     CDS             2466..2813
                     /gene="tnpB"
                     /locus_tag="ACHJ7J_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5002803.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLAKQVQHVLNVNPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLLTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            2833..4404
                     /locus_tag="ACHJ7J_004686"
     CDS             2833..4404
                     /locus_tag="ACHJ7J_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO5002804.1"
                     /translation="MDTSLDHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQDAQERISALQEEMAETLGEQYDPALPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSPLGCDVSEPLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVEVSRATLGRWTSAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRTLYESG
                     RITAAACMAHARRKIHDVHARAPTDITTESLQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWDSLNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDRGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIKDWPAN
                     RVRDLLPWKVALTSQ"
     gene            4457..>6841
                     /locus_tag="ACHJ7J_004687"
     CDS             4457..>6841
                     /locus_tag="ACHJ7J_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO5002805.1"
                     /translation="MNRIYSLRYSAVAKGFVAVYEFARKCVHKSVRRLGFPVLLLLPV
                     LFSAGSLAGTVNNKLGYQLFLDFAENKGMFRPGEKNIAIYNKQGELVGTLDKAAMPDF
                     SSTNTSGIATLINPQYVASVKHNVGYQNVTFGYKDSTSYILVDRNEHSNLDFHVPRLN
                     KLVTEVTPSNITAQGAINGAYLDKNRYPAFYRIGSGVQYIKDEEGNLTWLSGAYSYLT
                     GGTVGNPSSYQNGEMISTSSGLVFDYKLNGAMPIYGEAGDSGSPLFAFDTVQNKWVLV
                     GVLTAGNGAGGRGNNWAVIPLDFIGQKFNEDNDAPVTFRTSEDGTLEWSFNSSTGAGT
                     LTQGTTIYAMHGQQGNDLNAGKNLEFLGQNGQIDLKDSVVQGAGSLTFHNDYTVATSN
                     NSTWTGAGIIVDKGVSVNWQVNGVKGDDLHKIGEGTLTVQGTGINEGGLKVGDGNVIL
                     NQQSDNKGQVQAFSSVNIASGRPTVVLTDDRQVNPDNITWGYRGGILDVNGNDIAFHE
                     ISAADYGAQIYNSGNKNAAISLVKHDVIQWTGKDTQRILGRVYEYFNDETKKAEFFIL
                     NTTSTGWYPTPGEKYLPDMFRGADYFDTLEDADKEVKKNTQYIYHGKLTGNISFNPES
                     VGTFVMDGSADMSGTFTYENGRLTLQGHPVIHAYNTQSVADKLAASGDNSVLTQPTSF
                     SQEDWENRSFTFDRLSLKNTDFGLGRNATLNTMLEATDSTVTLGDERVFIDAFDGEGV
                     KYLLKEGVSQPEKSSDYSIFTGSTKINNTIVNINSIYYGDIFSNNSIINVNSPNT"
BASE COUNT         1925 a         1513 c         1594 g         1809 t
ORIGIN      
        1 gcttacccgt caccagtcgt atccttaata ccatactgtt ttaaccactg gcagggagtc
       61 accgagtggt attttttgaa actgtgaata aaatacgaca gactgttata accacacgcc
      121 ctggcaacaa caaaaatgtt accgtttccg caccgcatca gagttattgc ctttttcata
      181 cgtacttctg tgattatccg gctgaatcct gcaccttcac tgtgcagttt tcttttaagc
      241 tgactggcac ttatgcataa gacggatgcg acatcagaca gataccactt ctttgccggg
      301 tctgcggcaa caatggcttc aacacgcagc gaatagttat tcagtccaca tgataacaca
      361 ggcagtaact gtttttgatc attaagataa tccagcaata cgaataatgc agactcataa
      421 aggttaatat ttttctgttg ttccacaagt gacatcagtg agttcagaaa ggtaccatat
      481 gctttctttc tgaaaaaaga atttttcctg tcagtgatgc acaacgcatc ggcatccctc
      541 agtctgatat acagatagtc gctgatgata tgacagggta tatcaataaa cctcacacat
      601 tcttcacagg catccgtaat atactgaata tcatttcttt taagaagaag aatttcattc
      661 cgggtaaaat caaactcttc attgtaaaat gaagcacaga atggcctcac tataatggca
      721 aaacaatacg gaaacataga gtacaagttt tatttagaaa tatccggtgg tttttcttat
      781 tatagctgac agacttactt cctgacatta tattaagaag ttcggtttta ttattaaagt
      841 aaccggacaa aatatagtcc ggttaccgtg attatacgcg catataaatg ttatcagtta
      901 ccttcattca ggctgacagt atccgtaatc acaccatctt ctgcaccttt cagtgcaaga
      961 ttggcttcgt agtatttccc ttcactcatt aatttttgcg cactgttaac ctttttctgt
     1021 gtctgtttta atggcatcag ccactgcgtt tctgaaatat caattccggc aagatgcagt
     1081 acttcaattg cacctttttt gtcaccttta tccagttttt tattggcctc atcaatagct
     1141 ttctgttttt ccggggtaac agtaaagtct tcagacaaca ttaaactgct gttaataata
     1201 atgtagacat cgccttcctg tggtgtcttt ttatccttgc ggacaactgt ttcccatgtt
     1261 ttatcctcag atttaagcaa atcttcagcc cggtttatca gtctgtctgc tgttcctgta
     1321 tcaccactga ataaagcaag acgtgcatac tgcatgtcat ccataatttc cataccacgc
     1381 tgttctgcct tcagactcct ttctgataaa actgtctgag cctcattttt tgtagatgaa
     1441 gtgttttctg aagttgctga ggccattgca acagcagaag tcagggacag agcgattgct
     1501 acagcattaa aaatatgttt catatttttt actccgacat caggaagaaa gaatcatacc
     1561 tgacaataaa cataatatga tttttcccct gatgatatgg tcatatattc aggaaaccgg
     1621 ataaggatgc ccctcccgaa caggacactt atatgcccct atccggtata tatattttat
     1681 gtcctgcaac cgacagaaga aatctgacca cttattacat ccccaaaaaa ataaatacag
     1741 aaacgtaaat atcacaatgc gtcattatat aatcatactt acgttaccag taaccattat
     1801 accatcgcaa tcactcctaa aatccttaat aatgcactcc gctctgccga ataaaagaaa
     1861 atacatttca aatgaaatgt tgtgatatat gtattgtccg gagtgatggt atttttatga
     1921 agcagtttaa ataatacaga taattgtaac ttacctgtaa gcgtaaacct actgccgtat
     1981 gtagccatta agccactatt ggtaaggtag gtgcccgcct tttcaaacta gtgaacgcct
     2041 gttatggagc aaaaaacatt atctgcagaa ccccgcagat cattttcaaa taattttaaa
     2101 cttcaaatgg ttaaactggc tttacaacca ggatcctctg ttgcccgtat tgcccgggaa
     2161 cacgatatca atgataacct cctgttcaaa tggttcagac tctggcagaa cgaagggcgt
     2221 gttgcgcgtc gtctcccggt aacgacctct tctgacgctg gcattgaatt attatctgtg
     2281 gaaataacgc cggatgagca gaaagaaccg atggcgactc ttcccccgct tttatccact
     2341 ccttcacaga gcccagccag tgccagttcc tgtaaaggag agttccgtca cggtaaaatg
     2401 acgctggaaa atccttcacc agagctgctc acagtgttga tccgtgaact gagcgggaga
     2461 ggaagatgat ctcactccca tccggtaccc gtatctggct cgttgccggg ataaccgata
     2521 tgcgtaagtc tttcaacggg ctggctaaac aggtacagca tgtgttaaat gttaatccct
     2581 tctccggtca cctgtttatc ttccgcggac gccggggtga ccagataaaa gtgctgtggg
     2641 ctgacagtga cggactgtgc ctcttaacca aacgcctgga gcggggccgc ttcgtctggc
     2701 cggtcacccg cgatggaaag gtgcacctta ctccggctca gttgtccatg cttcttgagg
     2761 gcatcgactg gaaacatcca aaacgaacgg aacgcgctgg aatccgcata taacccgttg
     2821 taaagtgagg atatggacac ctcacttgat catgagaacg cccgcctgcg ggcactgttg
     2881 cagacgcaac aggacaccat ccgccagatg gctgaataca accgcctgct ctcacagcgg
     2941 gtggcggctt atgcttccga aatcaaccgg ctgaaggcgc tggttgcgaa actgcaacgt
     3001 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggca gatacaggat
     3061 gcacaggaac gaatcagcgc acttcaggaa gaaatggcgg aaacgctggg tgagcaatat
     3121 gacccggcac tgccatccgc cctacgccag tcttcagccc gtaaaccgtt accggcctca
     3181 cttccccgtg aaaccagggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
     3241 gaactcagtc cactgggatg tgatgtgtca gagccgctgg agcttatcag cagcgccttt
     3301 aaggttatcg aaacacaacg cccgaaactg gcctgttgcc gttgtgacca tatcgtgcag
     3361 gcaccggtgc cttcaaaacc cattgcacgc agctatgccg gagcggggct tctggcccat
     3421 gtcgtcaccg ggaagtatgc agaccatctg ccgttatacc gccagtcaga aatataccgt
     3481 cgtcagggtg tggaggtgag ccgcgccacg ctgggtcgct ggaccagtgc cgttgctgaa
     3541 ctgctggagc cgctgtatga cgtcctgcgc cagtatgtgc tgatgcccgg taaagtccat
     3601 gctgatgata tccccgtccc ggtccaggag ccgggcagcg gtaaaacccg gacagcccgg
     3661 ctgtgggtct acgtccgtga tgaccgcaac gccggttcac agatgccccc ggcggtctgg
     3721 ttcgcgtaca gtccggaccg gaaaggtatc catcctcaaa atcacctggc cggttacagc
     3781 ggtgtgcttc aggccgatgc ttacggtggc taccggacgt tatacgaatc cggcagaata
     3841 acggcagccg cgtgtatggc ccatgcccgg agaaaaatcc acgatgtgca tgcaagagcg
     3901 ccaaccgata tcaccacgga atccctgcag cgtatcggtg aactgtatgc catcgaggca
     3961 gaagtccggg gatgttcagc agaacaacgt ctggcggcaa gaaaagccag agctgcgcca
     4021 ctgatgcagt cactgtatga ctggatacag actcagatga aaacactgtc gcgtcactcg
     4081 gatacggcaa aagcgttcgc atacctgctg aaacagtggg atagcctgaa cgtgtactgc
     4141 agtaatggct gggtggaaat cgacaacaac attgcagaga acgccttacg gggagtagct
     4201 gtaggccgga aaaactggct cttcgcgggt tccgaccgcg gtggcgaaca tgcggcggtg
     4261 ttgtactcac tgatcggcac atgccgtctg aacaatgtgg agccggaaaa atggctgcgt
     4321 tacgtcattg agcatatcaa ggactggccg gcaaaccggg tacgcgatct gttgccatgg
     4381 aaagttgctc tgacctctca gtaaatatca atacggttct gacaagccgc ttacattttt
     4441 tcggaggtaa ttaaaaatga acagaattta ttctctgcgc tacagtgctg tggccaaggg
     4501 atttgttgcc gtatatgagt ttgcaaggaa atgtgttcat aagtctgtca gacgtttggg
     4561 gtttccggtt ttattactgc tcccagtact tttctctgca ggaagcctcg cgggaacggt
     4621 caataataaa ctcgggtatc agttatttct tgattttgct gaaaataaag gtatgttccg
     4681 accgggggaa aagaatatcg ccatttataa taagcaggga gaacttgtcg gtacgctgga
     4741 taaagcggct atgccggatt tcagttcaac aaatacttct ggaatagcaa ccttaattaa
     4801 tccacaatac gtagccagcg ttaaacataa tgttggttat caaaatgtta catttggata
     4861 taaagatagc acatcttata tacttgtaga cagaaatgaa cattccaatc ttgattttca
     4921 tgtcccacga cttaataaat tagtgacaga ggtgactcca tcaaatatta ctgcacaagg
     4981 agcaataaat ggagcatatc ttgataaaaa tagatatcct gcattttatc gcattggttc
     5041 aggagtacag tatattaaag atgaagaagg aaatttaacg tggttatcag gtgcatatag
     5101 ctatcttacg gggggaacag ttggaaatcc atcctcttat caaaatggtg aaatgatatc
     5161 aaccagttca ggtctggttt ttgattacaa acttaatggt gcaatgccca tttatggtga
     5221 ggctggtgac agtggttcgc ctttatttgc ttttgatact gttcagaata aatgggtgct
     5281 ggttggtgtt ctcactgcgg ggaatggagc gggaggccgg ggaaataact gggctgttat
     5341 tcctctggat tttatcgggc aaaaatttaa tgaggacaat gatgccccgg tcacattcag
     5401 aacatcggaa gacggtacac tggagtggag ttttaacagc agcaccggag ctggcacgct
     5461 gacacaggga accaccatat atgccatgca cgggcagcag ggaaatgatc tgaatgccgg
     5521 taaaaatctg gaatttctgg ggcagaatgg acagattgac ctgaaagaca gtgttgtaca
     5581 gggggctggt tctctgacat tccataatga ttatacggtt gcaacttcaa acaacagtac
     5641 ctggactggt gccggtatta ttgtggacaa aggcgtgtcc gtaaactggc aggttaacgg
     5701 tgttaaaggc gatgacctgc ataaaattgg tgaaggtacg ctgacggtgc agggtacagg
     5761 tattaatgag ggcggtctga aggtcgggga cggaaatgtt atactgaacc agcagtcgga
     5821 caataaagga caggtgcagg cgttcagtag tgtcaatatt gccagtggcc ggccgaccgt
     5881 agtactgact gatgaccggc aggtaaaccc ggataatatc acctggggat acaggggcgg
     5941 cattctggac gttaacggta atgatattgc ctttcacgag ataagtgcag ctgattatgg
     6001 cgcacaaatt tacaatagtg gtaataagaa tgcagcgatt tctctggtga agcatgatgt
     6061 cattcaatgg acaggaaaag atacacaaag aattttaggc cgggtttatg agtattttaa
     6121 tgacgaaaca aagaaggctg aattttttat attaaatacc accagtacag gatggtatcc
     6181 cacaccggga gaaaagtatt taccggatat gttcagaggt gctgattatt ttgatactct
     6241 tgaggatgca gacaaagagg ttaaaaaaaa tacgcaatat atttatcatg gtaaattaac
     6301 cggaaatatt agttttaatc cagagagtgt cggtacgttt gttatggatg gctctgcgga
     6361 tatgtccggt acattcactt atgaaaacgg gcgtctgacg ctgcaggggc atccggtaat
     6421 tcatgcatac aatactcagt ctgtagctga caaactggca gccagtggag acaattcggt
     6481 tctgactcag cctacgtcat tcagtcagga ggactgggag aaccgcagtt ttacctttga
     6541 caggttgtca ctgaagaata ctgattttgg tcttggtcgc aatgcaacgc tgaacaccat
     6601 gcttgaagca acagattcga cggtcacact tggcgacgaa agagttttta tcgatgcttt
     6661 tgatggagaa ggagttaaat atctcttaaa agaaggagtt tcacagccag aaaaatcaag
     6721 tgattatagt atatttactg gtagcacaaa aataaacaat actattgtga atataaatag
     6781 tatatattat ggggatatct tttctaataa tagcattatt aatgttaatt ctccaaacac
     6841 t
//
LOCUS       DAZFFG010000191         6778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFFG010000191 DAZFFG010000000
VERSION     DAZFFG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.192"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1864
                     /gene="espL4"
                     /locus_tag="ACHJ7J_004688"
     CDS             <1..1864
                     /gene="espL4"
                     /locus_tag="ACHJ7J_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO5002806.1"
                     /translation="EILPQRSDALDKQKLNLNSDRKEKEFPRVKLNGQCYFPGRPQNR
                     IVCRHIAAQYINDIYQNVNYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCV
                     AACGDFGLVMKAYFDKMESNDISVMATILLVDNHALTVRLRIKNTTEGCIHYVVSVYD
                     PNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLL
                     MLKGSVDEITPPLSPATMNLLMAIGQNHQLKQLMIQLQKMPELHRTEMLTAYNSINLP
                     GLYLAINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKD
                     KNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLDA
                     LPLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINI
                     SASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLL
                     ANNSNEYPGLFSAIQHKQQNVVEMVYLALSDHARLFGFTAEDIMGFWQHKAPQKYSAF
                     ELACELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1833..3569)
                     /gene="aceK"
                     /locus_tag="ACHJ7J_004689"
     CDS             complement(1833..3569)
                     /gene="aceK"
                     /locus_tag="ACHJ7J_004689"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO5002807.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPEHGWEALLMRVLTDL
                     PLRLPWQNKSRDINYIIRHLTETLGPENLSESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEDMHADLFRADYWRTLQNRIRAGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3753..5057)
                     /gene="aceA"
                     /locus_tag="ACHJ7J_004690"
     CDS             complement(3753..5057)
                     /gene="aceA"
                     /locus_tag="ACHJ7J_004690"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO5002808.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5087..6688)
                     /gene="aceB"
                     /locus_tag="ACHJ7J_004691"
     CDS             complement(5087..6688)
                     /gene="aceB"
                     /locus_tag="ACHJ7J_004691"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO5002809.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1716 a         1693 c         1640 g         1729 t
ORIGIN      
        1 ggaaatttta cctcaacgct cagacgctct tgataaacaa aaattaaatc taaactcaga
       61 tagaaaagaa aaagaattcc caagagttaa acttaatggt caatgttatt ttccggggcg
      121 cccccaaaac cgtatagtat gccgacacat tgctgcacaa tatattaatg atatttatca
      181 gaatgttaat tacaaacccc atcaagatga ttactcttca gctgaaaaat ttctcacgca
      241 cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt tccagccgtc ctgaggggcg
      301 ttgtgttgct gcctgcggtg atttcgggct agttatgaag gcatattttg acaagatgga
      361 atcaaatgac atcagtgtta tggctaccat attattggta gataaccatg ctttgacggt
      421 ccggctaaga ataaagaaca caactgaagg atgtatccat tacgtggttt cggtttatga
      481 tcctaatgta actaacgata aaataagaat catgagcgaa agtaaagagg atattaaaca
      541 ctattcactg atggatttta tgaatgtaga ttatagcctc ctgaaatggt caaatgatca
      601 tgttattaac caatctgttg caataattcc agcacttcca aaagaacagc tattgatgct
      661 aaaaggatct gtggatgaaa taacccctcc attatcacct gcaacgatga atttgctaat
      721 ggcaattggt cagaatcacc aacttaagca actgatgatt caactccaga aaatgccaga
      781 actacataga acagaaatgt tgactgccta taatagtatt aacctccccg gtttatattt
      841 ggctataaat tatggtaatg cggatatcgt tgagactatt tttaattcat tgtcagagcc
      901 aggatatgaa ggattactct cgaaaaaaaa tctcatgcat attctggagg caaaagataa
      961 aaatggtttt tctggattat ttttagcgat atcacgtaag gataaaaatg ttgtaacctc
     1021 gattctgaac gccttaccta aactggccgc aacacatcat ttagataacg aacaagtgta
     1081 taaattcctg agtgccaaaa atagtacgtc cagccatgtt ttataccatg ttatggcgaa
     1141 tggtgatgcc gacatgctga aaattgtttt ggacgcgtta cctttgttaa ttcgcacatg
     1201 tcatttgact aaagaacagg tactcgatct cctgaaggca aaggattttt atggttgccc
     1261 aggactatac ctggcgatgc aaaatggaca tagcgatatc gtgaaagtta ttcttgaagc
     1321 attgcccagc ctggcccagg aaattaacat ttcagcttcc gatattgtag atcttctgac
     1381 cgctaaaagt cttgcgcgcg acacaggttt gtttatggcc atgcagcgcg gacatatgaa
     1441 cgttattaat actattttta acgcattacc cactctgttt aatacgttta aattcgataa
     1501 aaaaaatatg aagcccctcc tcctggcaaa taattctaat gaatacccag gtttgttttc
     1561 agcgatacag cataaacagc aaaacgttgt agagatggtt tatcttgctt tatctgacca
     1621 tgcacgcctg tttggattta ccgctgaaga tattatgggt ttttggcaac acaaagcgcc
     1681 acaaaaatac tctgcctttg agttggcttg tgaattgggt caccgggtta ttgctgaatt
     1741 aatctttaat acattaaata agatggctga aagctttggc tttacggata accctcgata
     1801 cattgcggag aaaaattata tggaagcttt actcaaaaaa gcatctcccc ataccgtacg
     1861 ctaaatcttt gcctgcgccg atacgcataa acatcttcca catgtcctgc acgaatgcgg
     1921 ttttgtagtg tgcgccagta atcagcgcgg aacaggtcgg cgtgcatatc ttcaaagagt
     1981 ggcccaatgc gggggtcggc gcatagccag tggcggaact cttccgggaa aacatcgccc
     2041 ggcgagacgc tgtaccacgg ttcgctggca agttcgtctt ccggatagcg cggcggcggg
     2101 atgtcgcgga agttcacttc cgtcatgtag caaatttcat cgtaatcata aaaaaccaca
     2161 cgcccgtgac gggtgacacc gaagttttta aacagcatgt cgcccgggaa aatgttagca
     2221 gcggcaagct ggcgaatggc attgccgtac tcttcaatgg cgtcgcgcaa ctgctgacct
     2281 tccacttgtt ccagccagat attgagcggc accatccgcc gctcaatata aagatggcga
     2341 atcacaattt gttcgccgag atcggtgatt ttttccgctg cttcctggag cagtaattcc
     2401 attaatgccg gggaaatatg ccgcttctcc agcacaaagt tttcaaactc ctgggtgtcc
     2461 gccattcggc ccacgcgatc gtgctctttc accagttgat agcaggcacg aacgtgagcg
     2521 gcagacatct ctttctgcgg cgcgaacctg tctttgatga ctttaaatac acggtcaaag
     2581 cccggcagcg tgaacaccaa catcaccatg ccacgaatac ccggcgcttc aatgaactgc
     2641 tcattacagc cctgtagata aacgagatat tcgcggtagc tttcggtttt ggcgtgcttc
     2701 tggcagccga tagccatata caattcagcg gtggttttac ctggcagaat ttcccgtagc
     2761 cactcgacca gcgctgcggg cagcggtgca taaaccataa aataagaacg cgcaaagcca
     2821 aaaacaatgc tcgcttcggc ggtggtcgtc aggcaggtat caataaataa ctcgccgtcg
     2881 tccgtctggt ggatcggcag caaaaatggc aatgtgccgg aaggcgtgat cagtttgccc
     2941 accagccagg cggctttatt gcggtaaaac agttcgttcg ccacctgtaa atggctttcg
     3001 gagaggtttt ctggccccaa cgtttccgtc agatggcgaa taatataatt gatatcacgg
     3061 cttttattct gccagggcag gcgcagcggc aggtcagtga ggactcgcat cagcagcgct
     3121 tcccagccat gctcggggta gaagtctttc gccagtgggc gcggaatggt acgaaagcgg
     3181 cgctctggct gggagctaaa gataaaaagc cgctcgggag taagcgagcg gtggtcaaat
     3241 aaccgacagt acacggagtt aaaaaagctc tccgcaatct cgaagcgcgg gtaatccggc
     3301 aacagccggg tgtaatgctc tttgacgcgc agtaaaaatt ccgcgtccgt gctttggccg
     3361 ttggtaatgc agcgcagttg ctccacgacc agaccaacgt gatgatcgta aagatggata
     3421 cggtttttca tcgcctgctg gacagcatgc cagtcggcct gttcgaaacg ctgctgcgcc
     3481 ccggaagtca cttcgaggaa tcgaccatac tgagcgtcga agccttgcaa aatggtttga
     3541 gcaatcagta attccaggcc acgcggcatt tagcgccctc atcaggagca gagaattgcc
     3601 ggatgcggcg taaacgcctt atccagccta cgttcggcaa cggttgtagg catgataaga
     3661 cgcgcaagcg tcgcatcagg catcgtgcac cgattgccgg atgcggcgta aatgccttat
     3721 ccggcctaca gtcagcaacg tttgtaggtt gcttagaact gcgattcttc agtggagccg
     3781 gtcagcgcgg tgactgaaga cgtgccgccc tgaataatag tcgtcacttt atcgaagtaa
     3841 cctgtaccca cttcctgctg gtgagatacg aaggtatagc catctttcgc ggcggcaaat
     3901 tccggctgct gcactttctc aacgtagtgc ttcataccct cgccctgggc ataggcgttt
     3961 gccaggtcaa acatgttgaa ccacatgctg tggatacctg ccagggtgat gaactggaac
     4021 ttgtagccca tatctgacag ctgctgctgg aagctggcaa tagttttgtc gtcgaggttt
     4081 ttctgccagt tgaacgacgg cgagcagtta taagccagca gtttgcccgg atatttcgcg
     4141 tggatggctt gtgcaaagcg acgcgccagt tccagatccg gcgtggaggt ttcacaccat
     4201 accaggtcag catatggtgc atacgccagg ccacggctga tcgcttgctc aatgcccgca
     4261 tgagtacgga agaagccttc actggtacgc tcgccggtaa taaattcgct gtcatacggg
     4321 tcgcaatcgg aggtaatcag atccgccgca tcggcatcgg tacgagcaac cagtagggtt
     4381 ggaacgcccg tcacgtcagc tgccagacgc gccgcgacca gtttctgaat agcttcttga
     4441 gttggcacca aaactttgcc gcccatgtga ccgcatttct tcactgacgc cagctgatct
     4501 tcgaagtgaa ctgccgctgc accggcttca atcatcgctt tcatcagttc aaaggcattc
     4561 aggacaccgc caaaaccggc ttccgcatcg gcaacgatcg gcaggaagta atcgacatag
     4621 cgcggatcgc ccggctcaat gcccgcggac cattggatct gatccgcacg gcggaaagta
     4681 ttgttgatcc gctccaccac cgctggcacc gagttcgccg ggtagagcga ctgatccgga
     4741 tacatgctgg ccgccaggtt agcgtccgcc gctacctgcc atcccgacag atagactgct
     4801 tcaatacccg ctttcgcctg ttgcagcgcc tgaccgccag tcagtgcacc gaggctgttg
     4861 atgtagcctt ttttcgactc gccatgcagc agacgccaca ttttcgccgc gcccagttgc
     4921 gccagcgtgc attcaggatt gactgaaccg cgtaatttca ccacatcttc cgcgctgtat
     4981 gggcgagtaa tgccttccca acgcggttga gtccactctt tctgtaattc ttcaatttgt
     5041 tgtgtacggg ttttcatgtg cagatgctcc atagttatgt ggtggtttac gctaacaggc
     5101 ggtagcctgg cagggtcagg aaatcaatta actcatcgga agtggtgatc tgttccatca
     5161 agcgtgcggc atcgtcaaaa cgcccctggg agaaacgttc ttcgcccagt tcgctggcaa
     5221 tgactttcat ctcttcgccc agcatctggc ggaacaaggc tttagttacc ggtttgccat
     5281 tgctcaacat tttttgatga tggatccact gccagattga ggtacgggaa atttcagccg
     5341 tcgccgcatc ttccatcagg ccataaatcg gcacacagcc gttgccggag atccatgctt
     5401 cgatgtactg cacagccacg cgaatgttgg cgcgcatacc ttcttcggtg cgttcaccat
     5461 cacaaggtgc cagcagctga tcggcagtaa tcggcgcgtc ttgttcgcgc atcacttcaa
     5521 gctgattttt acgggagccg agaatgtcgt tgaataccgc cattgccgtg tccgcaaggc
     5581 ctgggtgagc gatccatgta ccatcgtgac cgttattggc ttccagcgat ttatccgctt
     5641 ttactttgtt gagcacctgg ttattgcgct cttcatcttt gctcggaata aacgccgcca
     5701 tgccgcccat cgcaaaagca ccgcgtttat ggcaggtttt aatcaacagg cgtgagtaag
     5761 cattcaggaa tggtttatcc atcgtcactg cctgtctgtc tggcaggacg cgatcgggat
     5821 agtttttcaa cgttttgata taactgaaga tgtaatccca acgaccgcag ttcagaccaa
     5881 caatatggtc acgcagtgcg tgaaggattt cgtccatctg gaacacggcg ggcagcgttt
     5941 caatcagcaa cgtcgccttg atggtgccgc gcggcagatt aaagcgatct tctgcatagc
     6001 tgaagacttc gctccaccag gccgcttcct gccaggactg ggttttcggc agatagaaat
     6061 agggaccact gccctttgcc aacagtgcct gatagttgtg gaagaaatag agcgcaaaat
     6121 caaacaggct gccggggatt gcctcaccac gccaggtgac atgtttttcc ggcaagtgca
     6181 gaccgcgtac ccgacaaatc aaaaccgctg gattgggctt gagctggtaa attttgcctg
     6241 cttcattggt gtaactgatg gtgccgttaa ccgcatcacg caggttaatt tgcccgtcga
     6301 tcactttgtt ccagtctggt gccagtgaat cttcgaaatc ggccataaag actttcacat
     6361 tggcgttgag cgcgttgatc accatcttgc gctctacggg tccggtaatc tctacgcggc
     6421 gatcttctaa gtccgcagga atcccgcgaa ttttccagtc agcatcacga atggaagctg
     6481 tttccgaaat aaaatcaggc aacgttccgt tatcaatatc ttgctgctgc tgaatgcgcg
     6541 ctgccagaag tttattgcgt tgtggcgtaa aatgcgtcac cagctcagtc agaaattcta
     6601 ccgcttcggc agtaagaatt tgcttctcct gctcgccata cggccttatg aaagccagtt
     6661 catcggttgt tgttgcctgt tcagtcatcg tgcagctcct cgtcatggat ccgaaaactt
     6721 ccccactgtg aacgaaggat tgttgtgcgc ttttcgttca gcacaactaa gactactc
//
LOCUS       DAZFFG010000192         6715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFFG010000192 DAZFFG010000000
VERSION     DAZFFG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.193"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..719
                     /locus_tag="ACHJ7J_004692"
     CDS             <1..719
                     /locus_tag="ACHJ7J_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5002810.1"
                     /translation="TAVFNTPEEFRIVTGNRTLGTVPVDSPLLPEQHIIFGGRRWKVT
                     EIEVEKKVIYVEATKGGQPPLFSGSGMSVHDVVRQEMLAIYRENDYRIAVGNKSVDYA
                     DAAARSLFAEGSNNFQRYNLQNDCFIASGQNCYVIPWMGDKIVNTITSLLIRCGFKAS
                     SFAGVIEVEDSNVASVQRALKKMLLSGLPSEFELAAIVPDKYLDKYDEYLPESLLAKG
                     FGAKAYDIEGTSIWLQKHLQ"
     gene            complement(768..1073)
                     /locus_tag="ACHJ7J_004693"
     CDS             complement(768..1073)
                     /locus_tag="ACHJ7J_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002811.1"
                     /translation="MISFHKNTRNLTVRSVPKKDIAHNIELIIRPLNISIEKYLSIEY
                     TNVEGIPLTSRRITIMGLKPGPKPIAKSTGKPDQRRRDNKDTPGNTPGLKPSKSTGK"
     gene            complement(1171..1695)
                     /locus_tag="ACHJ7J_004694"
     CDS             complement(1171..1695)
                     /locus_tag="ACHJ7J_004694"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO5002812.1"
                     /translation="MTQLGRNPSLLNHASEWITLSGQQITRLTELPPAYNMQRSAQLL
                     QQLRVLFPDNTRVQEMVDNWQKSVRSRALPEEAMTGWNEGMTRLQQLAERLNRLDEQR
                     GKYMTVSELKTEVFGIMQAFNRHIPVEEQLRRYGEARNQNGSEQQQKQAEMALNQLIN
                     RYLMEHTGNQERQP"
     gene            complement(1747..1896)
                     /locus_tag="ACHJ7J_004695"
                     /pseudo
     CDS             complement(1747..1896)
                     /locus_tag="ACHJ7J_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2227..2535)
                     /locus_tag="ACHJ7J_004696"
                     /pseudo
     CDS             complement(2227..2535)
                     /locus_tag="ACHJ7J_004696"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            complement(2590..2895)
                     /locus_tag="ACHJ7J_004697"
     CDS             complement(2590..2895)
                     /locus_tag="ACHJ7J_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002813.1"
                     /translation="MNPLFNDIQMRLFHLNHSPYSWHWNVRFRPQEAVYIGNDTCHIT
                     ITCNQSGFHLTRDGQRLFTESYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            complement(2961..4706)
                     /locus_tag="ACHJ7J_004698"
     CDS             complement(2961..4706)
                     /locus_tag="ACHJ7J_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5002814.1"
                     /translation="MTSGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQIICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVEQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPAEFSVKQLKDVMTQRGIPFATCFDSEGKKAYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVPSGVADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEYFA"
     gene            complement(4943..6151)
                     /locus_tag="ACHJ7J_004699"
     CDS             complement(4943..6151)
                     /locus_tag="ACHJ7J_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO5002815.1"
                     /translation="MVLILICVILWGIYVAVRALINLNERFIDAVSNPAGIIGLFFGL
                     LTVFAILLRFFIYRRLRKKTAAFEQAVSELVQRERDFNETVNAAIARGIRQEKEQLAR
                     RREEFHTARQKASRAMQRIVDSAWKFKAKTLLAGVTINNWQSKYDQLRKEREAYAAVS
                     EKIAFLNLEDNSDWESVRQQFLDKVALLEKAQEEKEYQAELKRQMREEKERQDELDRR
                     QREAEEEERRLAEQQKLIEEALRAAEGAHREELEKQRLELEQKIQEAHAQYERAKSMA
                     QLTKQGHVYIISNIGSFGEDVFKIGMTRRLEPMDRVKELSGASVPFDFDVHAMISCDD
                     APALEKTLHYSLEKYRINRINLRKEFFRVKLEKIINEVERHHGQVEYVADPAALQYLQ
                     SLEYAENEAA"
     gene            complement(6343..>6715)
                     /locus_tag="ACHJ7J_004700"
     CDS             complement(6343..>6715)
                     /locus_tag="ACHJ7J_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002816.1"
                     /translation="GLLFSVQADAARGRKPCSGAKGGISHCTSDGRFVCNDGSLSQSK
                     RFCSGYGMAPGKRQVKSPVTTKSVQKKKSAAVRKVSQPLVAGKNEAVPVTEPRKPTCA
                     PLYMASKPGFTHLPICSGNQH"
BASE COUNT         1643 a         1726 c         1543 g         1803 t
ORIGIN      
        1 ttactgccgt attcaataca cctgaggagt ttcgcattgt tacgggaaac cgcactcttg
       61 gtacagtgcc tgtagactcc cctcttttgc ctgagcaaca catcatcttt ggcggacggc
      121 gctggaaagt gacagagatc gaggttgaaa aaaaggttat ctatgttgag gcgactaaag
      181 gcgggcagcc accactattc agtggtagcg ggatgtctgt tcatgatgtt gttcgccagg
      241 agatgctcgc tatctacagg gaaaatgatt accgtatcgc ggtaggcaac aagagcgtcg
      301 attatgctga tgccgcagcc agaagcctct ttgcggaagg aagtaacaac ttccaacgct
      361 acaatctaca aaatgactgt tttattgcca gcggtcagaa ctgctacgtg atcccgtgga
      421 tgggggataa aattgtcaat acaattacgt ccctgcttat acgttgtggc tttaaagcca
      481 gttcttttgc aggtgtgata gaggtagagg attcaaacgt tgcctctgtc cagcgtgcac
      541 ttaagaaaat gctattgtcg gggttacctt cagaatttga gcttgctgca attgttcctg
      601 ataagtatct ggataaatat gacgagtatt taccagagtc tttgcttgcg aaagggttcg
      661 gggcaaaggc gtatgatata gaaggaacga gcatctggct gcagaaacac cttcagtaga
      721 ctggatatgt agtcagggta taaatgctta tttataccct tattttttta cttacctgtg
      781 gacttacttg gctttagtcc cggagtattg ccaggagtat ctttattatc acgtcggcgt
      841 tgatccggtt ttcctgtcga cttagcaatt ggttttggac ctggtttaag acccatgata
      901 gttatccttc gagatgtaag aggaataccc tcaacattgg tatattctat actgagatat
      961 ttctcaatgg aaatgttcaa cggtcgaatg attaactcga tattgtgagc aatgtctttt
     1021 tttggcacag agcgaaccgt tagattgcgc gtatttttat ggaaggagat catccaataa
     1081 cggcgttttt caaggaaatg aatctctttg attatctatg gtgtgcgcat tcttttgggt
     1141 ttattttcca ttaccatgtt ttatcagtca ttatggctgc ctctcctgat tgcctgtgtg
     1201 ttccatcaga taacgattaa tcaactgatt cagcgccatt tcagcctgtt tttgctgctg
     1261 ttcactgcca ttctggttgc gggcttcgcc atagcgacgt aactgctctt ctaccgggat
     1321 atgccggtta aaagcctgca tgatgccaaa cacctccgtt ttcagttcac tgaccgtcat
     1381 gtattttccc cgctgttcat ccagacggtt caggcgctca gccagctgct gtaagcgggt
     1441 catgccttcg ttccagcccg tcatcgcctc ttccgggagc gcacgactcc ttacactctt
     1501 ctgccagtta tccaccattt cctgtacccg ggtattatcg gggaagagta ccctcagttg
     1561 ctgcagcagc tgcgcactgc gttgcatgtt gtatgccggg ggtaattctg tcagtcgcgt
     1621 tatctgctga ccggaaaggg ttatccactc tgacgcatga tttaacagtg aggggtttct
     1681 gccgagctgt gtcatctggt ctgctgaaag cacatccggt ggtgatgtca gcagcaccct
     1741 gagttgctgc tctgccggtg atggctgtgt cagaaactgc catcccggaa tggccacgct
     1801 gaccgccagc aatgccgcac agacaccagc cacaaacggt ttcagaaacg tgggcgggga
     1861 tttttgaagg gagggcgtgg gttctgtttt tgtttcagaa acaacataca ccagacggga
     1921 gtgtggcaga gtctgatgtt cctgcacttc cggagcatca gaccggagcg cctgagccgg
     1981 aagcgttacc ggagaagata cttcaccctg cacaggctgg ttctccagac gggtgatata
     2041 tcccttcacc attgcttccg gtaaagccac tttactgctc tgtttaagtt cgtgccgggc
     2101 cagtgtgtca ctcagtgcct tcaggaatgt ttcggtctga tacagcaggg gcagattgtc
     2161 acggtcgtcc ggaggcatgg cccggagagt tttttgcaga cgattgaaca taccggtaat
     2221 aatttccagc cgggcgtggg tattcaacgg ccacatcact gaccagtgat gccgggtcag
     2281 cgccacaatc agagccagcc cttcattcag gccacttaac cctgttgtat gcatccgggc
     2341 tatggtgtac caggatgccg tctgtaattc cacgccattg tgttcataca gacgaaggca
     2401 gagcgtctcc acataacgcc agtccacatc agggcgtgcc gggtgggcca gtttgctgat
     2461 ttcatcgcgc agggcagcat aatccggtaa tgaccggggg tcaccacctg tttttagttt
     2521 cacttcagaa gtcatcggca gattcggtgt tgctgtatta aatcatgatt aacagttacg
     2581 gaaagaacat cagtgtgata ccgggacccg ggaaaggtat tccactgcgc ggatgattgc
     2641 cggtgtcaca tcccaccgcc gtttcagaac cggtaacagc tcattaaggt ttctgatata
     2701 actctcagtg aacagccgtt gtccgtccct ggttaaatga aaaccggact gattacaggt
     2761 aatggtgata tggcaggtat cattgccaat atatacggct tcctggggtc tgaatctgac
     2821 attccagtgc caggaatacg gggaatgatt aagatgaaaa agacgcatct gaatatcgtt
     2881 aaataacggg ttcatggcga attaaataaa cctgataata acagtatggt gaaagtccgg
     2941 aataaagaaa tggtccggta ttatgcaaaa tattcaaggt gacgactgat actgttttca
     3001 cggtaacagg tcaccagtaa ttcacgggtt gcccgggtaa acgccacata aagcaccgga
     3061 attacttcag actcatccgc tacaccagaa ggaacgaaag aagcattgat aaccgccaca
     3121 tacgggaact ccagcccctt actgctctgg tatgtcagta aatgcaccac atccttacgt
     3181 cgcgagtaag ccttcttacc ttcactgtca aagcaggtgg caaacggtat cccgcgttgt
     3241 gtcataacat ctttcagttg tttcactgaa aactctgccg gacacagaac agccatatca
     3301 ctccagtgac cgcacagggc atggcgcttc tccagccagt ctgcaacctg tctggcttca
     3361 tcacgttcag aagcacaacg caggatttcc ggctctgtgc cctcttcacc acccgcctgt
     3421 ggctgaacga gtggtatttc acggttctga tgtttttcaa aatactcgcg agagaatgca
     3481 taagcaaagt gcagaatgcg tttcggatta cgataattga ccggcaggac agaagtgcgt
     3541 ccctgtgcct gaatccccac actggccaga gaaaagttca gggcccgctc ccgtcggtaa
     3601 atggactggg catcatcgta catcaggagc agtgaacggg atgcgttatc aaacagacgg
     3661 gcaatcaggg ccagccagcg actgtcaaaa tcatgcccct catccaccag tacagcatca
     3721 tatccggttc gggtgatttt tccgctgttc acggcctctt ccagtgcact gaagcattta
     3781 tcaggatatt cttttctgtc ggccgtcacc ttaataccgt ggcgttttac cattgaggca
     3841 caccagctgt gaaaatggga taccgtaacc cgtgacgcca gccctctggc ttcaatgcat
     3901 tcccgcagat aactggccag ggtaatgttg tagcacaaca ccagaatctt tccgggtgtg
     3961 gtttcagcca ggtaaaggca acggaacaaa agaatcatgg tttttccgga gccggccaca
     4021 ccatgaatga cacgatgccc gtcaccaata tttctggcca gaatctcctg ctgaatatcc
     4081 atgattttga tttgtgagtt ctgttccacg gtcacttcag gaaacagatg ggcgcgcaga
     4141 atatctctca tccggggtgt cacccgggtt ctgaatcccg tggtaaacat cccggcaatt
     4201 ttttgctgga atacctcagg taaaacagac tgcgtcattt catcctggca gattatctgt
     4261 gctgaaggaa aaattttttc caccgcattc tcatcattcc ctgccagtgt cttcagttgc
     4321 tgacgggtaa tgcgggtaaa caccacacca tacgcccagg ccaggttcag ccgtccctta
     4381 tactgaccgt cattctgccg taagcacggg tttgcaggta aggcattaac cgtatcacag
     4441 gcataacgac gaacctgtac cagcggattg atttctgatt tcagaagacc gtcggtttcc
     4501 agcgtgacct gctcctgatt tacctggcgc agcgtggaaa ccgtccagtc cttgacctcc
     4561 agaaatacca gtccgttctc cgggtcaata atgacaaaat ccggatgccg gtttttccgt
     4621 cccaccggaa tatcgtacca gacaaggcag tcatcattca gaaagctctc cagtcgtgaa
     4681 gccaccctcc tctccccgga cgtcattctc tcctcagtgg acgccgatac atttatcagt
     4741 ttcgccataa acgcacctta atctgtgaac aaaaaaaata atcaccaccg acatccacgg
     4801 atgttatatc gtgggtgtcg ctttattcag aaataaaaaa cagaatcatt aacatgacca
     4861 gaatactgtg aaccatgctg cagggtatct gcagatatcc cttctgttat atccggctgg
     4921 ggtaaaatca gaacactgac tgtcaggctg cttcattttc cgcgtattcc agactctgca
     4981 ggtactgcag cgccgccggg tcagccacat attccacctg cccgtggtga cgctccactt
     5041 cattaatgat tttctcaagc ttcacgcgga aaaattcttt acgcaggtta atcctgttaa
     5101 tgcggtattt ttccagactg taatgaagtg ttttttccag cgcaggcgca tcatcacagg
     5161 aaatcatggc gtgaacatca aaatcaaacg gaacagaagc accacttaac tctttgaccc
     5221 tgtccatggg ttccagccgt cgggtcatgc caattttaaa gacatcctca ccaaacgaac
     5281 caatattcga aatgatataa acatgcccct gtttcgtaag ctgagccatg gatttagcac
     5341 gttcatactg cgcatgcgct tcctgaattt tctgttccag ctccaggcgt tgtttctcta
     5401 actcctccct gtgagcccct tctgctgcgc gcagcgcctc ctcaatcagt ttctgctgct
     5461 cagcaagacg ccgttcttcc tcttctgctt cccgctggcg ccggtcaagt tcatcctgac
     5521 gctctttttc ttcacgcatc tgacgcttaa gctctgcctg atattccttc tcttcctgcg
     5581 ctttttccag caatgccact ttgtccagaa actgttgcct gacactctcc cagtcagaat
     5641 tatcttccag gttaagaaag gctattttct cactgacagc agcgtaagcc tctctttcct
     5701 tacgaagctg gtcatatttg ctttgccagt tattaatcgt tacaccagcc agcagagtct
     5761 tcgccttaaa tttccaggcg gaatcaacaa tccgttgcat tgcacggctg gccttctgtc
     5821 tggcggtatg aaattcctca cgacgtctgg ctagttgctc cttttcctgt cgtattcccc
     5881 gggctatcgc ggcattgaca gtttcattaa aatcccgctc tctctgaacc agctctgaaa
     5941 cggcctgctc aaaggctgcg gttttctttc tgagtctacg gtaaataaaa aaacgaagta
     6001 aaattgcaaa tacagtaagc aaaccaaaaa acaaaccaat aatacctgcc ggattcgata
     6061 ctgcatcaat aaagcgctca ttaagattta tcagcgccct gaccgccaca taaatccccc
     6121 agagaataac gcaaattaaa atcaggacca atcccgctgc gttatcatct ttttttcatc
     6181 ctgccatgac tttcctttat tttccttctc actaactaaa tatctctgat taacgccctc
     6241 cggaaatatt tatacccgga aggctatctg gctttatgat gcccgccagt acagaatgaa
     6301 ctgcaggctg agcacagatt tctctgctat ttcagtgtca gattaatgct gattaccgga
     6361 acaaatgggc agatgcgtaa atccgggttt gctggccata taaagcggcg cacaggtggg
     6421 tttacggggt tcagtgaccg gtacagcctc atttttcccg gccaccaacg gttgtgacac
     6481 cttccggaca gctgctgatt ttttcttctg aaccgacttt gtggtgaccg gtgatttcac
     6541 ctgacgtttg ccgggtgcca tgccataacc ggaacaaaag cgcttcgact gactcagact
     6601 tccgtcatta cagacaaacc gtccgtctga cgtgcagtgt gaaataccac ctttagcacc
     6661 cgagcaaggt tttcttcccc gcgcagcatc tgcctgaacg gaaaataaaa gcccc
//
LOCUS       DAZFFG010000193         6540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFFG010000193 DAZFFG010000000
VERSION     DAZFFG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.194"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            44..331
                     /locus_tag="ACHJ7J_004701"
     CDS             44..331
                     /locus_tag="ACHJ7J_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005765007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5002817.1"
                     /translation="MKLKYSGLTASGTARPEIRNGDIYRDKYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVGHRKAVNQKRATGYIRKIREMLVAGGKK"
     gene            328..1725
                     /locus_tag="ACHJ7J_004702"
     CDS             328..1725
                     /locus_tag="ACHJ7J_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO5002818.1"
                     /translation="MKLAPNVKRLPKDKYTDAIIFAGIDAHSFAEHYIIAQAKKAGDP
                     VPPVYLGRYQLSELDNLQIVDDGRYRATVIRAGNIEEPQLLTIATKLAIAGVQEARLL
                     SENFELLEEWSDQLPRLRETWERGESLSNQRKTTLPMSVGSAGYDTQLDYVVKGIIPA
                     VSLCSIYGASGSYKSFLAGSWACHVSTGRQWGCRRVAHGAVLYVVGEGGIGVPRRVKA
                     WEVVHGEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCFG
                     GNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYRI
                     RREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRPPVE
                     LERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKLE
                     KDRVIRIDGDDVEPL"
     gene            1925..2176
                     /locus_tag="ACHJ7J_004703"
     CDS             1925..2176
                     /locus_tag="ACHJ7J_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002819.1"
                     /translation="MNKMRRDRTEPKYKALDMTEHALKVAIRTIDRHAGEGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            2173..2595
                     /locus_tag="ACHJ7J_004704"
     CDS             2173..2595
                     /locus_tag="ACHJ7J_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5002820.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGA
                     ATMWLSVMAFGRQADALAKHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQASNDDPYGDNIPF"
     gene            2844..3050
                     /locus_tag="ACHJ7J_004705"
     CDS             2844..3050
                     /locus_tag="ACHJ7J_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002821.1"
                     /translation="MAEEKKGGVSVYISSDIVEALKERHQQNVKAGIAAGLDPLAMVE
                     PSTGWQVRAYLRAALGMNQTHGGE"
     gene            3050..4105
                     /locus_tag="ACHJ7J_004706"
     CDS             3050..4105
                     /locus_tag="ACHJ7J_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5002822.1"
                     /translation="MAGKATALTTNQLFTYMNRGDIAEFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAITTGKNIIEGEGIERYE
                     IDWKIPGKNIIEQAKGRKWSEQDKETHDPIYDIDLYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            4117..4452
                     /locus_tag="ACHJ7J_004707"
     CDS             4117..4452
                     /locus_tag="ACHJ7J_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5002823.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSTQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            4446..4520
                     /locus_tag="ACHJ7J_004708"
                     /pseudo
     CDS             4446..4520
                     /locus_tag="ACHJ7J_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
     gene            4726..5268
                     /locus_tag="ACHJ7J_004709"
     CDS             4726..5268
                     /locus_tag="ACHJ7J_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5002824.1"
                     /translation="MKSHLMNKKTMAQSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IDNRVNNAINQVTNDKGDIDDDELLRVRIRLLTAQAEAQELKNERERGDVIDTGFCLY
                     ALSKLASQISSIMDSLPLTMQRRFPQITPAMLDGLKREVVKACNASATVADNLPKILA
                     DYLMETTGDVPEKLLQKKVE"
     gene            5524..5808
                     /locus_tag="ACHJ7J_004710"
     CDS             5524..5808
                     /locus_tag="ACHJ7J_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002825.1"
                     /translation="MTEAELLKVICRITGISQTAGKQEATQPDSVIAENYARVVAEVM
                     RRDGIELNGVDMRDIRIRVLELLSYRRRVEMYRESEKKTYQWKKPERLRT"
     gene            complement(6180..>6540)
                     /locus_tag="ACHJ7J_004711"
     CDS             complement(6180..>6540)
                     /locus_tag="ACHJ7J_004711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002826.1"
                     /translation="KQRGTNRHKKYTGGSSFQGEARQFMRGVNVDMGIPMTGLPGFFP
                     VKCQSGWWVLHTIDHRNDDPDVHLSTNGGKAHSRPPSLPQWVILEQSTGYALSAKKHI
                     SCKGNRYGCWWAASPSW"
BASE COUNT         1841 a         1500 c         1801 g         1398 t
ORIGIN      
        1 tacgcgaaca gaataaaacg ccttccaccg gagaataaaa ataatgaaac ttaaatattc
       61 tggcttaact gccagcggca ccgctcggcc tgaaatccgt aacggcgata tttaccgcga
      121 taaatatggc ggcatggtaa cgattaaagg cgtggcagaa cggcgcatca cctaccgccg
      181 tgaaggttac gaatatgatt gcgtgatgcc tgtttatcaa ttccggcgtg atttcacgct
      241 ggtaggccat cgtaaagccg ttaatcagaa acgtgcaaca ggttatatcc gcaaaatccg
      301 tgaaatgctg gtcgcagggg gtaagaaatg aaactggcac cgaacgtaaa acgattaccg
      361 aaagataaat acaccgatgc gattattttt gcgggtattg atgctcactc attcgcagag
      421 cattacatca ttgcacaggc caaaaaagca ggcgatccag tccctcccgt ttatctgggg
      481 cgttatcagt taagcgaact ggataacctc cagattgttg atgatgggcg atacagggcg
      541 acggtgatac gcgccggaaa tattgaagag ccgcagctgt taaccatcgc cacgaaactg
      601 gcgatcgccg gagtccagga ggcgcggcta ctttctgaaa atttcgaatt gctggaggaa
      661 tggagcgacc agcttccacg gcttagggag acatgggaac gcggggaaag cctgagcaac
      721 cagcgaaaaa ccacgctacc aatgagcgtg ggatctgccg gatacgacac acaacttgat
      781 tacgtggtta agggaattat tccggctgta tcgctttgca gcatatacgg ggcgagcggt
      841 tcctataaat cattccttgc cggatcgtgg gcgtgtcacg tttccactgg ccgccagtgg
      901 ggatgccgca gggtggcgca tggcgctgtt ctctatgtgg ttggtgaagg cggtatcggc
      961 gttcctcgtc gtgtaaaagc ctgggaggtt gtgcacggtg agcaggtgaa aaatctgtat
     1021 ctggtaaacc gtcccatctt tccggctgcc ccgcttgatg ttgatgaaat ggttatcgct
     1081 gcccgtcagg tggaacggga aacgggtaaa cctgtacgca tgattattct ggatacgctg
     1141 gcgcggtgct ttggtggcaa tgatgaaaat gattcccgtg atatgggggc gtttatccgt
     1201 gggtgtgacg aactgaaacg acgcacaggg gccacggtgc tggtggttca ccattccggc
     1261 aaggatgaaa caaagggagc gcgtggttcc agtgcatttc gtgcttcgct ggatgctgaa
     1321 taccggatac gcagggagga cgcaggaagc gaagccctgg ttatctcatg caccaaaatg
     1381 aaggatgcgg aggaactcaa agaagccgca tatgacttac gcgtggtgga gctttttacc
     1441 gacgctgacg gggagttaat cacgtcgctg gtggtggtgg ataagccgcg acctcccgtt
     1501 gaactggagc gcatcgagga ggctggcaac aagacggaaa accataccgc gctatgggga
     1561 tgcatacgtt cacgcacaca gaacggcgac aagtgcacga tcccgctgtt acgtgatgac
     1621 atgaaaaaac tggggtacga aatgaaaaac ttccggcgct ggctgtacaa gctggaaaaa
     1681 gacagggtta ttcgtatcga tggggatgat gtagagccgc tataaagtga ggtgcaaaag
     1741 cgaggggtat agaaggaggg ccaaaattag accgctaccc ctcacttttc ggcctgtata
     1801 catcctcaaa agtgaggggc aaaaaaatac ttatgaaaca cacacataga aaaagcgaaa
     1861 atctcaagcg agacggagcc agacacttta aaaagtgagg cgaaaaagtg agagtttgcg
     1921 agaaatgaac aaaatgcgta gagacagaac agagccaaaa tataaagcgt tagacatgac
     1981 agagcacgct ttaaaggtgg caatcagaac gatagaccgc cacgcggggg aaggatacgc
     2041 gaaggaacat cccgacctga taagcgcatt catgaccaca gcagcggcaa actttgccac
     2101 gttgacagaa cgggagattg ccgaagcgga acaggtaaca accatcaacg ttaaaaccgg
     2161 agagcagaca gcatgacagc acagatagcg gcttacggac ggctggtggc tgacccgcag
     2221 ttaaagacca ccagcaaggg tacacaaatg acgatggcga gtatggcggt cccccttccg
     2281 tgcagccagg cagatgacgg agcggcgacg atgtggttat ccgtcatggc gtttggcaga
     2341 caggccgacg cactggcaaa acaccacaaa ggcgaactgg tgagcgtggc gggtaacatg
     2401 caggtaagcc agtggacagg ccagaacggc gaaacgcggc agggctggca ggttatcgca
     2461 gacagcgtaa tcagtgcgag aacggcgcga cctggcggca aaaaaggcca gcaggggcag
     2521 gctactgacg cactgaacag ggcaaaacaa caggcgagta acgatgatcc gtacggggat
     2581 aacataccgt tttaagcaac gagtaacaga agccggagaa gtccggcttt tttatgcccc
     2641 aaaaaaagcc cgataaggtc agagggtctt atcgggcttt tgcatatgag gtttttttgg
     2701 tgcgctgaca cacatgatcg ggataataat ttcataattt gcaacataac tcaatattat
     2761 tgcacaaaat gcaatcctga ttataatcat gactggatga acatccagtt atgatttttt
     2821 aagtcgaaga ggaatttctt actatggctg aagagaaaaa aggcggtgtt tcggtgtaca
     2881 taagctccga catcgtggag gcgctcaagg aacgccacca gcagaacgta aaagcaggca
     2941 ttgcggcagg acttgatccg ctggcgatgg ttgagccgtc aacaggctgg caggtgcgcg
     3001 cctatttacg tgcggcgctg ggcatgaatc agactcatgg gggtgaataa tggcaggcaa
     3061 agcaacggca cttaccacta atcagctttt cacgtacatg aatcgcgggg atattgcgga
     3121 atttaaattc agtccgctgt ttaccacgct gtttttcccg aacgtggcga cattcagcac
     3181 ccaaaacatc atgctggata ccctggacat tgaagaagtc actatgtcgg cgttttgttc
     3241 gcctatggtt ggcagccagg tacagcgcga taaagggtac gaaaccagca caatcaaacc
     3301 tggctacatg aagccaaagc acgaaatcga tccaacaaaa accatcatgc gcatggctgg
     3361 agaagatccg gcacagctta acgaccctac ctaccgccgt atgcgcctga ttactggcaa
     3421 catgcgccgc cagataaacg ccattaaagc gcgcgtggaa tggctggcgg tgaatgcgat
     3481 aacgaccgga aaaaacatca ttgagggcga aggcatagaa cgctatgaaa tcgactggaa
     3541 gataccggga aaaaacatca tagagcaggc caaaggccgt aaatggtccg aacaggacaa
     3601 agaaacccac gatccaatct atgatatcga tctttatgcg gatcaagcag gttgccccgc
     3661 caacgtcatg atcatgggcg ctgaggtatg gcgaacgtta cgcagcttta aaaaattccg
     3721 cgaactgtac gatctttccc gtggttcaga gtccgccgca gaactggcct gtaaaaacct
     3781 gggcgaagta gtgagcttta aaggctatct gggcgatatt gcccttatcg tctattccgg
     3841 caaatacact gacagcgacg gcaccgaaaa atatttcctt gagcctgatt tactggtcct
     3901 gggcaacacc aacaacaaag gtctggtggc ctatggtgcg attatggatc aggaagctgt
     3961 aagaacgggc gcaacgcaaa acatgtacta cccgaaaaac tggattgagg acggcgatcc
     4021 ggcgattgag tacgtgcaga cacacagcgc accgcagccg gttccggctg atattcgcaa
     4081 atttgttacc gtcaaaattg gttaacgggg gattctatgg acactccata cattgagtta
     4141 tttgcaggca gtcagcaggt atccacgaca ctggtacatt ttgccgctga tgctggcgtt
     4201 attcaggaat ttaccccgct gatgctggcg gacaatggcg agtttaagcc gtgggatggt
     4261 caggaatctg gcaaggctgt ttatctgact tcgtacccag tggacacgtc aacgcagaaa
     4321 tcagcacagt gttacaagac ggggatattc aatattgccg ccgttaactg gcctgagagc
     4381 gtcgacaccg acgcgaaaaa atgcgccgcc tttgcgggtt ctggcgtatc cgttcagccg
     4441 ctagcgcgat aagcaggggg aacgatggca gaaacccggg cgatgacggc gacatcgatc
     4501 gcatactgaa aaccatctga gacaaggccg gagaaatccg gcttttttgc gggtcctttc
     4561 cggcatatgg acctgttacg gggcggcgac ctcgcgcgtt ttcgctattt atgagccttt
     4621 tcaggttggt ggtggtggtt ttgttgttcg ctgtatctct atgaataaaa agggaaagat
     4681 aaccacaaaa caccaacctg aagcattaac taagtgtggg tattgatgaa atcgcacctg
     4741 atgaataaaa aaaccatggc gcaaagctgc cgtgtaagtg ctacagcgtt cgacaagtgg
     4801 ggagtgactc ccgttgaacg taaaggccgt gaggcgtttt atgatgttgc cagcgtgata
     4861 gacaataggg ttaacaatgc aattaaccag gttacaaacg acaaaggcga tattgatgat
     4921 gatgaacttt tacgcgtaag aatcagatta ctgacagcac aggcggaggc gcaggagctt
     4981 aaaaacgagc gcgaacgcgg cgacgtgatt gatacaggtt tttgcctgta cgcgctttca
     5041 aagctggcga gccagatttc ttcaatcatg gacagtttgc cgcttaccat gcaaaggcgc
     5101 ttcccgcaga ttactcccgc tatgctggat gggcttaaaa gggaagtggt taaagcctgt
     5161 aatgccagtg ccacagttgc cgacaaccta ccgaagatac tggctgatta cctgatggaa
     5221 actaccggag acgtaccgga gaagttgcta cagaagaaag tcgagtaaca gacgcgcaat
     5281 tattgaacaa aactgagaaa cgacatgaaa gcgtcataaa tcgccatttt agatgattac
     5341 cgtgtcgttt ctttttattg tgtatctatt taaaaacaaa tagttatgtt cgagaattac
     5401 cgacatgctt tttccagaaa aattttcata aacagataaa aaccgcgagg tcgccgcccc
     5461 gtaacgggcc ataattccag gaaggacccg acgacaccag acaatcagaa catcgggaac
     5521 acaatgacag aagccgaact actgaaagta atctgccgta ttaccggaat cagccagaca
     5581 gcaggcaaac aggaggccac gcagccggac agcgtgatag ccgaaaatta cgcgcgtgtg
     5641 gtggctgagg tgatgcgccg tgatggtatt gagcttaacg gcgtggatat gcgcgacata
     5701 cggatcagag ttctggagtt gttgtcatat cgtcgccgcg tggagatgta tcgggagagc
     5761 gagaaaaaga cttaccagtg gaagaaaccg gagcggctgc gtacgtaatg cgtacggaca
     5821 tcgagacaaa gacgaacagt attctggttc agtgcaaaac aacgttaaac gtggttttgc
     5881 gtccagagtc atcgtaatgg ttttatgcgt cctgcgttgc gctggtggat ttgagggata
     5941 tagctggtag tggtaaatcc cttttgcgtg gcggtgcgat ggctgcatgg tggaaatcac
     6001 cacgaagggg aggtgataaa cactccccct tttcgtgtgg tgcatggtca aaatgaccac
     6061 ggaggggaga accgactatc tcaaagatag tcaccccata ccaggatcac gctaataacg
     6121 cacaacagag tgacccatag ccaccctgtt caaccgttcc ggctgactgg acagcatcat
     6181 caccatgatg gtgacgcagc ccaccagcaa ccataacgat ttcctttaca gctgatgtgc
     6241 ttttttgctg ataaggcgta accagttgat tgttccagaa tcacccattg ggggaggctg
     6301 ggcggcctgc tgtgggcttt tccaccattg gtggaaaggt gcacatctgg atcatcatta
     6361 cggtgatcaa tggtatgtaa aacccaccag cctgattgac atttaaccgg aaaaaagcca
     6421 ggtagccccg tcatggggat ccccatatcg acattaacgc ccctcatgaa ttggcgcgct
     6481 tccccttgga aagatgaccc gcctgtatat ttcttgtgtc tatttgttcc acgttgtttc
//
LOCUS       DAZFFG010000194         6483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFFG010000194 DAZFFG010000000
VERSION     DAZFFG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.195"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(80..2113)
                     /gene="betT"
                     /locus_tag="ACHJ7J_004712"
     CDS             complement(80..2113)
                     /gene="betT"
                     /locus_tag="ACHJ7J_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO5002827.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2242..2829
                     /gene="betI"
                     /locus_tag="ACHJ7J_004713"
     CDS             2242..2829
                     /gene="betI"
                     /locus_tag="ACHJ7J_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO5002828.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIASRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2843..4315
                     /gene="betB"
                     /locus_tag="ACHJ7J_004714"
     CDS             2843..4315
                     /gene="betB"
                     /locus_tag="ACHJ7J_004714"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO5002829.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPVE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4329..6017
                     /gene="betA"
                     /locus_tag="ACHJ7J_004715"
     CDS             4329..6017
                     /gene="betA"
                     /locus_tag="ACHJ7J_004715"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO5002830.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWQEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQGALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1595 a         1781 c         1760 g         1347 t
ORIGIN      
        1 aatatatgca taatttttta cagcgagtca atgattcccg cgccatatgc aaacatggcg
       61 cggttttatg caataacaat cacgcgtccg ggaacatcac gctatggccc ggcgcttcac
      121 gatggagatg aataaagtta aggtgccgct cgtactggtc aagaatatcg gtgatcacct
      181 gctctttgct gtagtccatc aggtcgttgc cctggctgcc ttctaacagg aaagtttcca
      241 gccggtagta agtcgattta ccgctacgtg cgcggtaggt aaagcccggc accgaatatt
      301 gctgcggcca aatctgatag acaaagtttt gctcttcgcc catatgcacc aacaaatcca
      361 gatgccccaa ctgttgtccc tcttccggcg gcaggctttt tagctccacg tacgcgccgc
      421 gcaaccgcaa ctcctgcgcc acttcttcca ttgccgggta acagaccgtc tccatcatct
      481 gtttagtgta acgcgtgccc ggataattca tcaggcgcga gagacgtttt ttccagctca
      541 ggcgatcctg aagccccagc ggtcgcggtg cggtatcgcg gttggcactt tcacggcggt
      601 aatcttctac cttcagagat ttatacaacc ccgccatcac gaagaagatc acaaagctga
      661 acggcagccc cataatcacc gtggtgtttt gcagcgcgga tatcccgttg gtcatcagca
      721 tgccgagcgt cagcaggcca atcgccaccg accagaagac gcgcagccag ccgggggcgt
      781 cgctgttgat atctttaagc tgcgaggtga aattccccag caccagcgcc ccggagtccg
      841 ccgaggtcac ataaaacagc aggccagtaa tggtggcgac ggaggcgcta aaggtaaacg
      901 ccggatactg cgccagcagg ctgtagaagc cgcgctccgg atggaccatc gcttcctcgg
      961 caaatgccgc gccgccgtgg atgatttcat acagcgcgct attgccgaac accgagagcc
     1021 ataacagcgt gaaggtaaac ggaataatca acgtgcccag cacgaactgg cgaatggtac
     1081 gcccacgcga gatacgcgcc aggaacaagc cgacaaacgg cgaccatgcc acccaccatg
     1141 cccagaagaa gagcgtccag ttattcatcc actcaactgg acggtcgaag gcaaaactgt
     1201 tgagcgtcat gcccataaag cgattcacat agtcgccaac attcagcacc agtgcattaa
     1261 gcaggaacga agtgtcgccc ataaacaaca cgaacaggat caatcccagc gccagcgcaa
     1321 cgttaagttc cgataacaca cgaatgccct tatcgacacc ggatgtcacc gagatagtgg
     1381 cgattatcac cgacaaggcg atcagtgccg cttttgccgc catcgaatcg ggaatatcaa
     1441 acagtacgct caagccatag ttaagctgca ccacaccgat accgagcgta gtggcaatac
     1501 cgaagatagt gccgatcacc gctgcaatat ccactgagtg acctatcggc ccgttaatcc
     1561 gtttaccgaa gatcgggtac agcgccgagc ggatggtgag cggcaaatta taacgatagc
     1621 taaagtatcc gagcgccatg cccatcagcg catacatcga ccagccggtt aagccgtagt
     1681 gaaacagcgt ccagaccatc gcctgacgcg cggcctcaat cgtctgtccc gcgccttccg
     1741 gcggctgcat atactgcgtt accggttcgg ctacggaaaa gaacatcaga tcgataccga
     1801 tcccggcagc aaacagcatc gccgcccaac tcagcaggct gaattccggt ttggattgtt
     1861 ctggcccgag cttcaccgaa ccaaaacgcg aacaagcgat acagaccaca aagacaatat
     1921 agagcgttgc cgccagcaga tagtaccaac cgaaggtttt agaaacccag tccagcgtgc
     1981 ggccaatcca cagggccgag aagtcgcgaa acaggatcgt tgtcagggaa aacaacaaaa
     2041 tcagtccggc ggaggtgtaa aacaccaccg gattgatttt gtccttttcc ctgctgtgtg
     2101 aaaggtctgt catccagtat ccccactgtt attgttacca tttaaaatca aattcgtaac
     2161 aattaagaca cattttatat tgaacgtcca atcaataacc gctttaatag ataaacaacg
     2221 ctgatgaatg gagtggcgaa aatgcccaaa ttggggatgc agtcgatccg gcgcagacaa
     2281 ctgatcgacg ccacactgga agcaataaat gaagtgggca tgcacgatgc aacgatcgcg
     2341 cagatcgcca gccgtgcagg cgtttctacg gggatcatca gccactattt cagggacaaa
     2401 aatggtctgc tggaagcaac catgcgcgat atcaccagtc agctgcgtga cgcggttttg
     2461 aatcgattac atgcacttcc gcagggcagt gcagagcagc gattacaggc gattgttggc
     2521 ggaaacttcg atgaaacgca ggtgagcagt gcggcgatga aagcctggct ggcgttctgg
     2581 gccagcagta tgcatcagcc gatgctctat cgtttacagc aggtcagcag ccgccgcttg
     2641 ctgtcgaatc tggtgagcga gtttcgccgc gaattgccgc gccaccaggc acaggaagcg
     2701 ggctacggcc ttgccgcgct gattgacgga ttatggctgc gcgcggcact gagcggcaaa
     2761 ccgctggata aacctctcgc acactcgctg acccgtcact ttattactca gcatttaccc
     2821 accgattaac cgaggagacg tgatgtcccg aatggcagaa cagcagcttt atatacatgg
     2881 tggttatacc tccgccacca gcggtcgcac tttcgagacc attaacccgg ccaacggtaa
     2941 cgtgctggcg accgtgcagg ccgccgggcg tgaggatgtc gatcgcgccg tgaagagtgc
     3001 ccaacagggg caaaaaatct gggcggcgat gaccgcgatg gaacgctcgc gtattctgcg
     3061 tcgggccgtt gatattctgc gtgaacgcaa tgacgaactc gcaaaactgg aaaccctcga
     3121 caccggaaaa gcatattcgg aaacctcaac cgtcgatatc gttaccggtg cggacgtgct
     3181 ggagtactac gccgggctga tcccggcgct ggaaggcagc cagatcccgt tgcgtgagac
     3241 gtcatttgtt tatacccgcc gcgaaccgct gggcgtggtg gcggggattg gcgcatggaa
     3301 ctacccgatc cagattgccc tgtggaaatc cgccccggcg ctggcggcag gcaacgcaat
     3361 gattttcaaa ccgagcgaag tcaccccgct taccgcgtta aagctggctg aaatttacag
     3421 cgaagcgggc ctgccggacg gcgtatttaa cgtgttgccg ggcgtgggcg cggagaccgg
     3481 gcaatatctg accgagcatc cgggcattgc caaagtgtca tttaccggcg gtgtcgccag
     3541 cggcaaaaaa gtgatggcta actcggcggc ctcttccctg aaagaagtga ccatggaact
     3601 gggcggtaaa tcaccgctga tcgttttcga tgatgcggat ctcgatctcg ccgccgatat
     3661 cgccatgatg gcaaacttct tcagctccgg tcaggtgtgt accaatggca cccgcgtctt
     3721 cgttccggcg aaatgcaaag ccgcatttga acaaaagatt ctggcgcgcg ttgagcgcat
     3781 tcgcgcgggc gacgttttcg atccgcaaac taacttcggc ccgctggtca gcttcccgca
     3841 tcgcgataac gtgctgcgct atatcgccaa aggcaaagag gaaggcgcgc gcgtactgtg
     3901 cggcggcgat gtactgaaag gcgatggcct cgataacggc gcatgggtcg cgccgactgt
     3961 gttcaccgat tgcagcgacg atatgaccat cgtgcgtgaa gagatcttcg ggccagtgat
     4021 gtccattctg acctacgagt cggaagacga agtcattcgc cgcgctaacg ataccgacta
     4081 cggcctggcg gcgggaatcg tgacggcgga cctgaaccgc gcgcatcgcg tcattcatca
     4141 gctggaagcg ggtatttgct ggatcaacac ctggggcgaa tccccggtag agatgcccgt
     4201 tggcggctac aaacactccg gcattggtcg cgagaacggc gtgatgacgc tccagagtta
     4261 cacccaggtg aagtccatcc aggttgagat ggctaaattc cagtccatat tctaaccagg
     4321 aggtttattt gcaatttgac tacatcatta ttggtgccgg ctcagccggc aacgttctcg
     4381 ctacccgtct gactgaagat ccgaatacct ccgtgctgct gcttgaagcg ggcggcccgg
     4441 actatcgctt tgacttccgc acccagatgc ccgctgccct ggcattcccg ctacagggta
     4501 aacgctacaa ctgggcttac gagacggaac ctgaaccgtt tatgaataac cgccgcatgg
     4561 agtgcggacg cggtaaaggt ctgggtggat cgtcgctgat caacggcatg tgctacatcc
     4621 gtggcaatgc gctggatctc gataactggg cgcaagaacc cggtctggag aactggagct
     4681 acctcgactg cctgccctac taccgcaagg ccgagactcg cgatatgggc gagaacgact
     4741 accacggcgg cgacggcccg gtgagcgtca ccacctccaa acccggcgtc aatccgctgt
     4801 ttgaagcgat gattgaagcg ggcgtgcagg cgggctaccc gcgcacggac gatctcaacg
     4861 gttatcagca ggaaggtttt ggcccgatgg atcgcaccgt cacgccgcag ggccgtcgcg
     4921 ccagcaccgc gcgtggctat ctcgatcagg ccaaatcgcg tcctaacctg accattcgta
     4981 ctcacgctat gaccgatcac atcatttttg acggcaaacg cgcggtgggc gtcgagtggc
     5041 aggaaggcga cagcaccatt ccaacccgcg cgacagcgaa taaagaggtg ctgttatgtg
     5101 caggtgcgat cgcctctccg cagatcctgc aacgttccgg cgtgggtagc gctgaactgc
     5161 tggcggagtt tgatattccg ctggtgcatg atttacccgg cgtcggcgaa aatcttcagg
     5221 atcacctgga gatgtacctg caatatgagt gcaaagaacc ggtttccctc taccctgccc
     5281 tgcagtggtg gaatcagccg aaaatcggtg cggagtggct gtttggcggc accggcgttg
     5341 gtgccagcaa ccactttgaa gcaggcggat ttattcgcag ccgagaggaa tttgcgtggc
     5401 cgaatattca gtaccatttc ctgccagtag cgattaacta taacggctcg aatgcagtga
     5461 aagagcacgg cttccagtgc cacgtcggat cgatgcgctc gccaagccgt gggcatgtgc
     5521 ggattaaatc ccgcgacccg caccagcatc cggcgattct gtttaactac atgtcgcacg
     5581 agcaggactg gcaggagttc cgcgacgcaa ttcgcatcac ccgcgagatc atgcatcaac
     5641 cagcgctgga tcagtatcgt ggccgcgaaa tcagccccgg cacggaatgt cagacggatg
     5701 agcagctcga tgagttcgtg cgtaatcacg ccgaaaccgc cttccatccg tgcggtacct
     5761 gcaaaatggg ctacgacgag atgtccgtgg ttgacggcga aggccgcgtg catgggctgg
     5821 aaggcctgcg cgtggtggat gcgtcaatta tgccgcagat tatcaccggg aatttgaacg
     5881 ccacgacaat tatgattggc gagaaaatag cggacatgat tcgtggacag ggagcgttgc
     5941 cgaggagcac ggcgggatat tttgtggcaa atgggatgcc ggtgagagcg aaaaaaatga
     6001 gtcgtgattt gaactgactc aagaaccgat cagattgaga gttaccgttc caggtatggg
     6061 gacagaatcg ttatataaac attgaggtaa ctctcatgct tcatagtgac aatctcgcca
     6121 gcaaattcta agctgacttg ctcaatctgg ctgatgaagg tgctatgcat acacagataa
     6181 actacagatg aaataccact cagcttaaga attacgccgc taatgttcct tgctatggtt
     6241 acctgttcat tcccgctcac ggttagcatg tacgaaaagg gttcttgtaa cgagtacaaa
     6301 aataagtcag accaaaacca catggagctt taaacacggt tcgtataatt tattagcagc
     6361 gatttatttt tactcttctt taattaatca tcatgcttaa atatttttta gcacaagatt
     6421 atcgcttcgc taacatttta ttttacattg atttccaatt gataataaaa agttgttaat
     6481 aag
//
LOCUS       DAZFFG010000195         6282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFFG010000195 DAZFFG010000000
VERSION     DAZFFG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.196"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(92..399)
                     /locus_tag="ACHJ7J_004716"
                     /pseudo
     CDS             complement(92..399)
                     /locus_tag="ACHJ7J_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537868.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
     gene            complement(439..669)
                     /locus_tag="ACHJ7J_004717"
                     /pseudo
     CDS             complement(439..669)
                     /locus_tag="ACHJ7J_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(817..1041)
                     /locus_tag="ACHJ7J_004718"
     CDS             complement(817..1041)
                     /locus_tag="ACHJ7J_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002831.1"
                     /translation="MKVVSLFIHVLAVVMISVMTVHLCSMAAEKVPEECDIPVRVAAL
                     VVLWLVFSGLIYWLFTAFMKIDVNVVYSHE"
     gene            complement(1071..1262)
                     /locus_tag="ACHJ7J_004719"
     CDS             complement(1071..1262)
                     /locus_tag="ACHJ7J_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002832.1"
                     /translation="MSDFVRHFSVKDKKEDVVMQDMHFLLEDFICGHISRKTGRVPDR
                     LRQRMDKLRLQRVRKDRTG"
     gene            complement(1376..2431)
                     /locus_tag="ACHJ7J_004720"
     CDS             complement(1376..2431)
                     /locus_tag="ACHJ7J_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002833.1"
                     /translation="MIFRYIRLFLVVLCSVVLVDRGNAASLFNITFVGNAEQTITPSS
                     AYTLTHAMDNLPYVFDEAGVTIGYSAVTVWQYPRGVRVCAGLDAKVGLPVVGSINGQD
                     IYGVTDEVGLLVWMGDAGYSDDVAMTGNTWTNVLDSWCTANVPPTSQGLSLYVKPVIL
                     KRSTTASYVIPQTTIGSIKFRLEDGPNSGYETTVNFTLSSFTINNTVTSCRLLTPASV
                     NVALQDVFVSQFPSSGDEVAAGSTTLRLQCDAGVTVWATLTDATTPSNRSDILTLTGA
                     STATGVGLRIYKNTDSLPLKFGPDSPVKGNENQWQLSSGTETLPSVTLNVKYVNTGEG
                     INPGTVNGISTFTFSYQ"
     gene            complement(2436..4904)
                     /locus_tag="ACHJ7J_004721"
     CDS             complement(2436..4904)
                     /locus_tag="ACHJ7J_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5002834.1"
                     /translation="MLSVPLWAAEDAEFDPSFLSRTPGSSVIDVRRFTHGNPVPSGNY
                     YSDIYLNGEWKGNAELSFRDMSSSQEESVLCVTPELLSILDVSPEAVAREPDTGNGRC
                     MPFSAVVPDAKIRFDLSLLRLNVEIPQAMLVSRPRGYISPSQWQAGVPAAFVSYDVNH
                     WRYRTPDMENNQSYLGLRAGFNAGGWAFRHRGTESWSDGHAEGYRSIETNLQHDIAML
                     RAQLTLGDFTTTGELMDSNSLRGIRLASDDRMLPGSLRGYAPVVRGMAGSNARVTVRQ
                     NGNIIYETTVPAGPFSISDLYPSGYGGDLTVTVTESDGQTRSFIVPFASVAQLVRPGF
                     SRWQVAAGRYRYGNRTFSDTVFQGTLQYGLTNDITLNTGMSTAPHYLSGLGGVAFNTP
                     LGAVATDITLARTTFPGSDTTHRGYSLHAGYSARIPETSTNVTLAAYRYSSRDFWSLR
                     DAILTRNRDVINDSSVRSATGYRPRNQLQLTVNQSLGEGWGNLYLTGATYHYWGHTGT
                     FNEYQAGYNNVWKKMSYQIGFSQTRRSGDYHHDNRFFFSFSLPLSDTGRTLLSTTVNV
                     SDKNSNSIQSSISGVSGDDNQLSYGVSLNTQENGPTGYAVNGSYRSPVVTLQATAGND
                     SRHNRQISAGISGAIVAHPFGVTASNDLGDTFTVIHADGAGGAVINNAPGNRLDPWGN
                     GIVPYVTPYEKNYISIDPTALAADVELSATEREIIPRANSTTMVTFTTKTGKAMLFDV
                     KMPDGTPPPMAAEALTADGKSAGYIAQGGRLFVRDLDAEKGILRIVWGNGQNDSCVIH
                     YSASGENARGNTAPVIHPATCMRE"
     gene            complement(4988..5710)
                     /locus_tag="ACHJ7J_004722"
     CDS             complement(4988..5710)
                     /locus_tag="ACHJ7J_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5002835.1"
                     /translation="MKHILLFIFTVLLSLPSYGSVVIMGTRVIYPAEQKSINVRLNNG
                     DSTPSLIQAWLDTGDPSSPPDSVMVPFIITPPVFRMEPLSGQTMRIMYTGEKLPADRE
                     SLFWLNVLDIPAKPSFAGKSEKAQGYNYLQFAVRSRIKFFFRPDGLPFSPDDAYKKVT
                     WQLTGGDTLQASNPTPYFITFSTVSVGQGAQKVSSTQGGMAAPFSSVTFRLKQKVSPS
                     GKVEWTVVNDNGGYQKGESVVK"
     gene            complement(5779..>6282)
                     /locus_tag="ACHJ7J_004723"
     CDS             complement(5779..>6282)
                     /locus_tag="ACHJ7J_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002836.1"
                     /translation="AASSSATLAYDGTITFNGKVVAQTCSVSSGSKNLTVTLPTVSEA
                     SLAAATNTAGLTPFTIELTGCDTNAASGAQNVKAYFEPNATTDYDTGNLNIAASGANN
                     VQIQLLNADGVTPIKLGQDATGQNVTAVQIDNAAMKLRYNAQYYATAQATAGDVSATV
                     NYTIAYQ"
BASE COUNT         1668 a         1695 c         1341 g         1578 t
ORIGIN      
        1 gtttactgat ttattgaaag taaaaagcgc aatatatccg gcctctttca catatatccc
       61 acttcagaag ccagccacag agagcttggt atcagtagta tgatggtctt tttctgaaca
      121 tctcttctga cagatctgca attcccatac gcttcagggt ctcatctgca gcttttgccc
      181 cccacggata ttcatctttt cgtttaaaac atgagaagtg gtctgcacta tgcagtattc
      241 ccgtcgtcgt aaaagccact cccgctgtgc atctgtaata tctgacgccc ccatcacgac
      301 cagccatcgt gcagaagccc tgcgccagca tcctgcaacc tcaagcttca gcgcaataag
      361 gtcttccacg acttccgggg tactcttttt cctgctcaaa acatcaactc actgaaataa
      421 tactcatatc tgacggtctc agcgaaccgt ccatgccggg ctgaaatcct cttcctgaaa
      481 cagctccgtt agcccgctaa tctgtttcaa cctgagaact tcatccttgt ccatacccag
      541 ctccgtacca atacgttcat ccgtccagcc cagccgcgac aggtccctga caatatcaga
      601 cattgaagtt atctggtgtt tgcccctggc ccggttatgt cggatggtcg cagcaatacg
      661 tgaggcctct gtaaatatat cctgtctcat aaaacccggg gaatgcccct gaatacagga
      721 cagtgaaaac aggcatgata gtcaggaaag ccacgcctgt tacagaagaa agtctcccgc
      781 agggataatg cataatcaga cacttcttgg tcaaagttat tcatgtgaat atactacatt
      841 cacatcaatt ttcataaagg ctgtgaacag ccagtaaatc aggccactga ataccagcca
      901 cagcacaacc agggctgcaa ccctcacagg gatatcacac tcttccggca ctttttccgc
      961 agccatactg cacagatgga ctgtcataac agaaatcatc accacagcaa ggacatgaat
     1021 gaaaagagaa acgactttca ttttccggaa atacatatac aaaccctgac tcagcctgtc
     1081 ctgtctttcc tgaccctctg taatcttaac ttatccatgc gctgacgaag tctgtccgga
     1141 acacgtcctg tctttcttga aatatggcca caaataaaat cttcaagcag aaaatgcatg
     1201 tcctgcatta ccacatcttc ttttttgtct ttcactgaaa aatgcctgac aaaatcgctc
     1261 atgaaaaatg tccctgaaac gctgttatcc gccttcagaa accggtactg ctttcaccaa
     1321 cgcaaatcat tttgttgtta ctgtccgacc tgttctcccc tcccgaaact cgctgttact
     1381 gataggaaaa cgtaaatgtt gatattccgt tgaccgtacc cggattaatt ccctcaccgg
     1441 tattaacata ctttacattc agcgtaactg agggcaacgt ttccgtccct gacgataact
     1501 gccactggtt ttcatttccc tttaccggcg aatcaggtcc aaacttcagg ggcaaactgt
     1561 cagtgttctt gtatattctc agcccgactc cggttgcagt cgatgccccc gtcagtgtga
     1621 gtatatcgct tctgttggac ggtgtggtcg catcagtcag tgttgcccat accgtcactc
     1681 ctgcatcaca ctgcagccgc aacgtcgtgg agccggctgc aacctcatca ccggaagagg
     1741 gaaactgact gacaaaaacg tcctgcaatg caacattgac ggatgccggc gtcaacagtc
     1801 tgcacgatgt gactgtattg ttaatcgtaa aactactgag tgtaaaattg acagttgttt
     1861 catatccact atttggccca tcttccagac gaaacttaat actacctatt gtcgtctgag
     1921 ggataacata agaagcagta gtacttcttt taagaatgac aggttttaca taaagggata
     1981 gtccctggct cgttggagga acgtttgctg tacaccatga atccagtaca tttgtccacg
     2041 tatttccggt cattgcaaca tcatctgaat atccggcatc ccccatccat accagcagcc
     2101 ccacttcatc agtgacgcca tagatatcct gaccattaat gctacccact acgggtaaac
     2161 caacttttgc atcaagcccg gcacacactc ttacccctcg tggatattgc cagacagtaa
     2221 ccgcggaata accaatagtt actcccgcct catcgaacac gtaaggcaga ttatccattg
     2281 catgcgtcag tgtatatgca gatgatggtg tgattgtttg ttcagcatta ccgacaaacg
     2341 tgatattaaa caaagatgct gcgtttcccc tgtcaaccag aacaacactg caaagtacaa
     2401 ctaaaaataa tctgatatat ctgaaaatca tggtattatt ctcgcataca tgttgcagga
     2461 tgaataaccg gagcagtatt tcccctggca ttttctccgg acgcactata gtgaatgaca
     2521 caactgtcat tctgcccatt tccccagact atcctgagta tccctttttc agcatccagg
     2581 tcacgtacaa ataaccggcc tccctgagca atgtagcctg cagatttccc gtctgctgtt
     2641 aatgcttccg cggccatcgg cggcggcgtt ccgtccggca tcttcacatc aaacaacatg
     2701 gctttccccg tttttgttgt gaaggtcacc atggtggtac tgttggcccg gggaataatt
     2761 tcccgctctg ttgcagacag ttccacatcc gcagccagcg ctgtcgggtc gatactgata
     2821 taattctttt cgtaaggtgt gacatacgga acaatgccgt taccccaggg gtccagccgg
     2881 ttacccggcg cattattaat caccgcccca ccggcaccat cagcatgaat aacggtaaat
     2941 gtgtcaccaa ggtcattact ggccgtcaca ccaaacggat gcgccactat tgccccggat
     3001 ataccggcag atatctgacg gttatggcga ctgtcattcc ctgccgtggc ctgcagggtg
     3061 accaccggtg aacgataact gccattcacc gcataccctg tcggtccgtt ttcctgtgtg
     3121 ttcagggaca caccatatga cagctgattg tcatccccgc tgacaccact gatactgctc
     3181 tgaatactgt tactgttttt atcgctgaca ttcactgtcg tggacagcaa cgtccttccc
     3241 gtatctgaaa gtggcaggga aaaactgaaa aagaaccggt tatcatgatg ataatcacca
     3301 ctccgtcggg tctgggaaaa gccaatctgg taactcattt ttttccagac gttgttataa
     3361 ccggcctggt actcattgaa tgtccctgta tgcccccagt aatgataagt ggctccggtc
     3421 agatacaggt ttccccatcc ctcaccaaga ctctggttca cagtcagttg caactgattc
     3481 cttggccggt aaccagttgc actcctgacc gatgaatcat taatcacatc acgattcctg
     3541 gtcaggatgg catcccgaag actccagaag tcacgggatg aataacggta agccgccagc
     3601 gtcacatttg tactggtttc cggtattcgt gcactgtaac cggcatgcag gctgtagccg
     3661 cgatgtgtcg tgtcagaacc cgggaaagtc gttctggcca gcgtaatatc cgtggcaaca
     3721 gcccccagtg gtgtgttgaa tgccacacca cccagccctg acagataatg aggtgcggta
     3781 ctcatccccg tattcagggt gatatcattt gtcagcccgt actgcagggt cccctggaat
     3841 acggtatcgc tgaatgtcct gttaccgtaa cgataacgcc cggccgccac ctgccagcga
     3901 ctgaaccccg ggcgaaccag ttgtgcaacc gaagcaaacg gcacgataaa actcctggtc
     3961 tgtccgtctg actctgtcac agtgactgtc aggtcaccac catatccact gggataaagg
     4021 tcactgatgg agaaaggccc tgccggcacg gtagtttcat aaataatgtt accgttctgc
     4081 cggacagtga ccctggcgtt acttccggcc atcccccgaa ccacaggggc ataaccacgc
     4141 aacgaacccg gcagcatccg gtcatcagaa gccagtctta ttcccctgag gctgttactg
     4201 tccatcagct cacctgtggt ggtgaaatcc cccagtgtca gttgcgcacg caacatggct
     4261 atgtcgtgct gaaggttcgt ttcaatactc cggtaacctt ctgcatgacc gtcactccag
     4321 ctttctgtcc cccggtgacg aaatgcccag cctcctgcgt taaagccggc acgaagacca
     4381 agataactct gattattttc catatcgggc gtccggtaac gccagtgatt cacgtcataa
     4441 ctgacaaagg cagccggcac cccagcctgc cactgagaag gtgatatgta tccccgtgga
     4501 cgggaaacca gcatggcctg cggaatttca acattcagcc gcagcagtga caggtcaaaa
     4561 cggatttttg catcaggtac aactgcagaa aatggcatac aacgaccatt tccggtatcc
     4621 ggttcacggg caacggcctc gggtgacaca tccagaatgg ataacagttc cggcgtcaca
     4681 cacagaacac tctcctcctg tgaagaggac atatcacgga atgacaattc cgcatttcct
     4741 ttccattcac cgttcagata aatgtctgaa taataattcc ctgagggaac aggattcccg
     4801 tgcgtgaaac gacggacatc aatcacactg ctccccggcg tgcggctcag aaaactcggg
     4861 tcaaattctg catcctctgc cgcccacaaa ggcacagaca gcatatacag tcccgacaga
     4921 caacataaat aaatgacacg gtacttcaga ccggataaag aataatgact cacttacggg
     4981 gcccttatta tttcacaacg gattcgcctt tctggtaacc accattatca ttcaccaccg
     5041 tccattccac ttttcctgac ggagaaacct tctgcttcag tctgaaagtg acagaagaaa
     5101 acggtgcggc cattcccccc tgagtactgc tgactttctg agctccctgc ccgacactga
     5161 cagtactgaa ggtaataaaa taaggcgtgg gattactggc ctgaagggta tcaccgcctg
     5221 taagctgcca tgtcactttc ttatatgcat catcaggaga aaaaggcaga ccatcaggac
     5281 ggaaaaaaaa cttaatccgg cttcgtacgg caaactgaag gtaattatat ccctgtgctt
     5341 tttcagattt tccggcaaaa gaaggtttgg ccggaatatc aagaacattc agccagaaca
     5401 gtgattccct gtctgccgga agtttttctc ccgtatacat aattcgcatt gtctgtccgg
     5461 aaagcggctc catccggaaa acaggaggcg taataataaa cggcaccatg acagagtcag
     5521 gaggggaaga tgggtcccct gtatcaagcc atgcctgtat cagggatggc gtactgtctc
     5581 cgttattcag ccggacatta atactttttt gttctgcagg ataaataacc ctggtcccca
     5641 taataaccac actgccatat gagggcagag acaaaagaac ggtaaaaatg aacagcagaa
     5701 tatgtttcat acaggctccg taaacacggg gcaacaatgc cccgtgaaca cttaagaagg
     5761 caggtattaa gataaaaatt actgatacgc gatggtgtaa tttactgtgg cagagacatc
     5821 accagctgta gcctgagctg tggcataata ttgtgcatta taacgcagtt tcatagctgc
     5881 gttatcaatc tgtacagctg taacattctg tcctgtagca tcttggccta gttttattgg
     5941 agtaactcca tctgcattta gaagctgtat ctgaacgtta tttgcaccac tggcagcaat
     6001 atttagatta cccgtatcgt aatcagtcgt ggcgttaggt tcaaaataag cttttacgtt
     6061 ctgagcacca gaagctgcat tggtatcaca cccggtcagc tcaatggtaa atggagtcag
     6121 acctgcagta tttgttgcgg cagccaatga agcttcagaa acagtcggta atgtaacggt
     6181 taaattcttg cttcctgatg aaacagaaca agtctgagca acaacttttc cattaaaagt
     6241 aattgtaccg tcataagcaa gcgtagcaga agatgacgca gc
//
LOCUS       DAZFFG010000196         6268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFFG010000196 DAZFFG010000000
VERSION     DAZFFG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.197"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..110)
                     /locus_tag="ACHJ7J_004724"
     CDS             complement(<1..110)
                     /locus_tag="ACHJ7J_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO5002837.1"
                     /translation="MARPPKAPAYLDEIAVRQWKEKSRQLSGREDLTPAD"
     gene            complement(230..415)
                     /locus_tag="ACHJ7J_004725"
     CDS             complement(230..415)
                     /locus_tag="ACHJ7J_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002838.1"
                     /translation="MAMMTEQEQTRLIRGLIRQRDTWKTQETEHKANRTGRTKRTTAK
                     RLTDRDREVMECFRNRW"
     gene            complement(399..839)
                     /locus_tag="ACHJ7J_004726"
     CDS             complement(399..839)
                     /locus_tag="ACHJ7J_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5002839.1"
                     /translation="MPWQPLRRCTEPGCNKRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRALYLSKNPLCVRCLAKGIYTPALVVDHIIPINGGGDVLFWPEWNHQAL
                     CQTCHNRKTTREDPATKANRKAGMYREQEERAAHRNDWMYGDDD"
     gene            complement(839..1141)
                     /locus_tag="ACHJ7J_004727"
     CDS             complement(839..1141)
                     /locus_tag="ACHJ7J_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5002840.1"
                     /translation="MSEARITPDEVRAHLRLDDDLSGEGELLKMYTDAALEACQKHIG
                     KRFEDGLEFTPAMRVGCLMYIAFLYENREAVSPVEHSELPMAISALWSVYRDVGVY"
     gene            complement(1134..2348)
                     /locus_tag="ACHJ7J_004728"
     CDS             complement(1134..2348)
                     /locus_tag="ACHJ7J_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5002841.1"
                     /translation="MWWPFSRKKSEQRNLSIDDFLALSGVPNTGSGEYVSAGTAESLP
                     AVMNAVSVIAEAVATMPCYLYLVRNDKGREAREWLDSHPVDILLNEQPNSCQTPYQFK
                     RTMMRHCLLNGNAYAVIEWGQDGQPKSLHPYAPGCVVPERTGAHKYRYTITEPYTGTV
                     RTYLQEEVLHLRYASDDGFLGRSPVTICREALGLGLAQQRHGASIMKDGMMAAGIITS
                     GEWLDGVKGKQALDALERYKGAKNAGKTPILEGGMDYRQLGMSNQDAEWLASRRFSIE
                     DIARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKAALLVASPVP
                     GTRYLVEFDSADLLRATPTERYATYEKGIKNGIMNPNEAREREGMPPREGGDEFSQAW
                     KQTVEIKGRKDE"
     gene            complement(2350..2910)
                     /locus_tag="ACHJ7J_004729"
     CDS             complement(2350..2910)
                     /locus_tag="ACHJ7J_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5002842.1"
                     /translation="MKNTDFEIRTSELTASNKKLVGYAVRWNSLSEIIWDEFREQFTP
                     GAFADYLAAGNDVRCLYEHDYTQLLGRTKSGTLVLTEDNTGLRFELTPPDTQLGKDVL
                     TLVERGDITGMSFGFRALCEEWSIAQKPYLRTVTAAELREITITSMPAYPESGVEIAH
                     RSLFAQHPELCPTGNNRHRWSELAGL"
     gene            complement(2965..4134)
                     /locus_tag="ACHJ7J_004730"
     CDS             complement(2965..4134)
                     /locus_tag="ACHJ7J_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5002843.1"
                     /translation="MKKLIELRQQKNALKNQMRSLLEKADSENRSLNDDEGKQFDELR
                     AKADSLDTDISRLESVADEERSKPGTGIQKVSSDELRNYIVTGDVRSLSTSTDSGRDG
                     GYTVIPELDREVMRQLQDDSVMRVIATVKTAKSNEFQKLVSTGGATVGRGTEGSARSE
                     TNTPKIERVTIKLNPIYAYPKTTQEILDFSEVDILGWLSSEIADTFASTEEDDFVNGN
                     GNGKPKGFMAYTRAATSDKTRAFGTIEKMVAASGTAITADELIDILYKLKAKYRKNAV
                     WVMNSGTAGTLQKLKNENGDYIWRDSLKEGAPDMLLGRPVYCLESMPDIGAGKAPLAV
                     GDFSRGYFIVDHVTGIRTRPDNITEPGFYKVHTDKYLGGGVVDSNAIKILEMKAG"
     gene            4394..5158
                     /locus_tag="ACHJ7J_004731"
     CDS             4394..5158
                     /locus_tag="ACHJ7J_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002844.1"
                     /translation="MTKEILAVPGMRPSPLFKRTYKLVREIDERLTKLIHRWRFFNAG
                     PPMKVTAYDGTEICYQGLAFKGTPVQIFWNGFIDPYIENNSEIVLEQTSALAIECQFS
                     IEESIEEAKLLLLVMIRRLYYEMAETDKILRGDGFSFPEKKDVRGYIESISQKIIEHA
                     EIEKLKKTHPKQNIFNIATVNSQYVQLGTNNNINIQELSEFFSKIASTGEKEIITLSK
                     NLLKKAYSKNLLSKENYEALISNLNTQTKYTFKSTS"
     gene            5188..5697
                     /locus_tag="ACHJ7J_004732"
     CDS             5188..5697
                     /locus_tag="ACHJ7J_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002845.1"
                     /translation="MDLNVMAYPEKFTIAGVEYKGKRNSAEKKVLIPYTEEPDINIGD
                     EITQKLGKGEIILKVIDMSFLPGGTLNVGTNHPHMLTLKVENLTANEHKPKQSSQNTF
                     NIGNISGTQVQLGEHNNLIVNLSITELVEKVSQSQDPQAKNLLKDLLNNSTVASLVGA
                     GASALLTML"
     gene            complement(5732..5992)
                     /locus_tag="ACHJ7J_004733"
     CDS             complement(5732..5992)
                     /locus_tag="ACHJ7J_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002846.1"
                     /translation="MPILTHEINNYFDQFGLHDIASMPTTQYRQALANGGLLWIDHHD
                     FIRSTLSDEILATNQEQVNVLIEHLQVLRKKMPLPPNWMSEK"
BASE COUNT         1605 a         1580 c         1396 g         1687 t
ORIGIN      
        1 cagtcggcgg gggtaaggtc ttcccgcccg gaaagctggc gcgatttttc cttccactgc
       61 ctgaccgcga tttcatccag gtaagcgggg gcttttggtg gtcttgccat gttctttttt
      121 cgcccaatta ttttcaaaaa aattaccgtg cacaaaaatt tgagggggcg gtcggtgtcc
      181 ggcagggacg gtttcgtcct gaaaaccacc cccaccccct ctgacggcct caccagcgat
      241 tgcgaaaaca ttccatgacc tcgcggtcac ggtcggttaa tcgcttcgct gtggtgcgtt
      301 ttgtgcgccc tgtcctgttg gctttgtgct ctgtctcctg tgtcttccat gtgtcacgct
      361 gccttatcag tccacgtatc agcctggttt gctcctgttc agtcatcatc gccatacatc
      421 cagtcgttac ggtgtgccgc ccgttcttcc tgctcgcgat acatgcccgc tttacggttt
      481 gctttcgtgg ctggatcttc ccgtgtcgtc ttacggttgt ggcacgtctg gcacaatgcc
      541 tggtgattcc actcaggcca gaagagaaca tcaccgccgc cattgatggg aatgatgtga
      601 tccaccacaa gagctggcgt ataaatcccc ttagccagac aacgcacgca taacgggttt
      661 ttgctcaggt acagggcgcg gtatttgtcc cactgtcggg aatacccgcg cgcgcggcgg
      721 tgtccccgtc tggcatcctc tgcacgccat gcagcccgcc tgtgctcttc acacttgccg
      781 gacttcacgc gcttattaca gccaggctca gtgcatcgcc ttaatggttg ccacggcatc
      841 agtacacccc tacgtcacga taaactgacc agagcgcaga aatagccata ggcagttccg
      901 agtgctccac tggtgaaacc gcttcccggt tctcgtacag gaaagcgatg tacatcaggc
      961 aaccaacacg cattgccggg gtaaattcca gcccgtcttc aaaacgtttc ccgatatgct
     1021 tctggcaggc ttccagcgcc gcatcggtat acattttcag aagttcgcct tcaccggata
     1081 aatcatcatc aagtcgaaga tgtgccctga cttcatcagg tgtaattctg gcttcactca
     1141 tcttttctcc ctttaatttc cacagtctgt ttccatgcct ggctgaactc atcaccacct
     1201 tcacgcggcg gcataccctc acgctcacgg gcttcgttcg gattcatgat cccgttctta
     1261 atccctttct catacgtggc ataacgttcg gtgggtgtgg cgcgtaataa atcggctgaa
     1321 tcaaactcaa ccagataacg ggtaccaggt acgggagaag ccaccagcaa agcggccttg
     1381 atttgctgtt cgaagttcgc cagccacggg cgcattgtca tggtcagaaa cgcgcggctt
     1441 gcctcgctga aattgctgta ggtgctgttg ctgtattcct gcagaaaaat aggcgatacg
     1501 ttgaacatgc gggcgatgtc ttcaatggag aagcgacggg aggccagcca ttccgcatcc
     1561 tggttactca ttcccagttg cctgtaatcc atgccccctt caaggattgg cgtttttccg
     1621 gcatttttcg cccccttgta gcgttccaga gcatccaatg cctgtttacc tttcacgccg
     1681 tccagccatt cgcctgacgt gataatccct gccgccatca tgccatcttt cataatgctg
     1741 gctccgtggc gctgttgagc aaggccaagc ccaagtgcct cacggcaaat cgtgacgggg
     1801 gagcgcccca gaaagccatc atccgaggca tagcggagat gcagaacttc ttcctgtaaa
     1861 tacgtgcgca ccgttcctgt atagggttcg gtgatggtat agcggtattt gtgtgcgcct
     1921 gtgcgttccg gtacaacaca ccccggcgca taaggatgaa gtgattttgg ctgcccgtcc
     1981 tgcccccact caataaccgc ataggcgtta ccgttcagca ggcagtgacg catcattgtg
     2041 cgtttaaact ggtaaggtgt ctggcacgaa ttaggctgct cattcagcag aatatctacc
     2101 gggtgactgt ccagccattc ccgcgcctcc ctgcccttgt cattacgtac cagatacaga
     2161 taacacggca tcgtggccac cgcctcagcg atgacagaaa ccgcattcat cactgcaggc
     2221 aatgattcag ccgtcccggc agaaacatat tctccggatc cggtattcgg tacgccggac
     2281 agcgccagaa aatcatcaat ggacaggtta cgctgctcgc tttttttacg actaaaaggc
     2341 caccacatat cacaaccccg ccagctcaga ccagcgatga cgattatttc ctgtcgggca
     2401 taattcaggg tgctgtgcaa acaacgaacg gtgggcaatc tccacgccag attctggata
     2461 agcaggcatc gacgttattg tgatttcacg gagttcagcg gcggttacgg tacgcaaata
     2521 cggtttttgc gcgatactcc actcctcgca taatgcgcga aaaccaaagc tcattcctgt
     2581 aatgtcgcca cgctccacca gcgtaagcac atcttttcca agctgggtat ccggcggtgt
     2641 cagttcaaaa cgtaacccgg tgttatcctc agtcagtacc agagtgccgg atttggtgcg
     2701 ccccagcagt tgggtatagt catgctcata caggcagcgc acatcattac ccgccgccag
     2761 atagtcagca aaagcccccg gcgtgaactg ttcgcggaat tcgtcccaga taatttctga
     2821 aaggctgttc cagcgaacgg cataccccac cagcttttta ttgctggcgg tcagttcaga
     2881 tgtgcggatt tcaaaatcgg tgtttttcat cggtgtactc cataaagctg aaaaaggagg
     2941 ccgcagcctc ctccttactc atgactagcc agctttcatt tccagaattt tgatggcgtt
     3001 tgaatccacc acaccaccgc ccagatattt atccgtgtgg accttgtaga atccgggttc
     3061 agtaatgttg tccggtcggg tgcgaatccc tgttacatga tcaacgatga aataaccacg
     3121 actgaaatcg ccaaccgcta gcggtgcttt tcctgcgccg atgtccggca tggactccag
     3181 gcagtaaaca ggacgaccaa gcaacatatc cggcgcacct tctttaaggc tgtcgcgcca
     3241 gatataatcg ccgttctcat ttttcagctt ctgtagtgtc cctgccgtgc ccgagttcat
     3301 cacccagacg gcatttttgc ggtatttcgc tttcagcttg tagagaatgt cgatcagttc
     3361 gtccgctgta atggcggttc cacttgccgc taccattttt tcaatggtgc caaaagcgcg
     3421 ggttttgtca ctggtcgccg cacgggtgta agccatgaag cctttcggct tgccgttacc
     3481 gttgccatta acaaaatcat cctcttcggt gctggcgaac gtgtcggcaa tttcggagga
     3541 taaccagccc agaatatcca cctctgaaaa atctaggatt tcctgcgtgg ttttcgggta
     3601 ggcgtagatc gggttcagct tgatggttac gcgttcaatt ttcggggtgt tggtttcact
     3661 gcgtgcgctg ccttctgtgc ctcgtcctac agttgcgccg ccagtggaaa ccagtttctg
     3721 aaactcattt gattttgcag tcttcacggt cgcgatcacg cgcataacac tgtcatcctg
     3781 tagctggcgc atgacttcgc gatcaagctc aggaattacg gtatatccgc catccctgcc
     3841 gctgtcagtg ctggtggaca gagatcgcac atctccggtt acgatgtagt tacgcaattc
     3901 atcagatgat actttctgga tgcccgttcc tggcttgctg cgttcttcat cagccacaga
     3961 ctcgaggcgg gaaatgtctg tatcgaggga atcagctttt gcacgcagtt catcaaactg
     4021 tttgccctcg tcatcgttca ggctgcggtt ttcactgtcg gctttttcca gcagggatcg
     4081 catctggttt ttcagggcgt ttttttgctg gcggagctcg attaatttct tcatgaaggt
     4141 tttctcgtat tggttaagat tcaggacgtg aaaccaacac ggagggagcg ccgcccgaca
     4201 ctctcggcat ctcgcagatc aacccggcat cgcgcagggg atcggacggc aacagtggcg
     4261 gctcacgtct gagtgccact atttaagata tggatcaaaa aattgatgta aataaggtat
     4321 ttttgttacg aacagtcttg aatgttaaca cacaaataat ttacaaaaat gctgataaac
     4381 tatggagatt caagtgacaa aagaaattct tgctgttcct gggatgcgac catccccatt
     4441 gttcaaaagg acttataaat tagttcggga gattgacgaa agattaacca aactgattca
     4501 tagatggcga tttttcaatg caggcccccc catgaaagtt actgcatatg atggaacaga
     4561 aatatgctat caaggtttgg catttaaagg tactcccgtt caaatttttt ggaatggttt
     4621 tatcgatcct tacattgaaa ataactcaga aattgtttta gagcagacct ccgcgttagc
     4681 aattgaatgt caattctcaa tagaggagtc aatcgaggag gctaaattac tattacttgt
     4741 gatgattcgc aggctttact atgaaatggc cgaaacagat aaaatattaa gaggtgatgg
     4801 gttttccttc ccagaaaaaa aagacgttcg tggttatatt gaaagtatct ctcaaaaaat
     4861 catagaacat gctgaaattg aaaagctaaa aaaaacgcat ccaaaacaaa acatattcaa
     4921 catagctact gtaaatagcc aatacgtaca acttggtaca aataacaata ttaatataca
     4981 agagctctct gagttcttct caaaaattgc atcaactggt gaaaaagaaa ttattactct
     5041 ctcaaaaaac cttcttaaga aggcctactc aaaaaactta ctgagtaaag aaaactatga
     5101 agctctaata tcaaacctta acacacagac aaaatacact ttcaaatcta ctagttaaac
     5161 gcaaaagcga tcacgaggag caacataatg gatcttaacg tcatggcata tccagaaaaa
     5221 ttcacaatcg caggagttga atacaagggg aaaagaaact cagctgaaaa aaaagtgcta
     5281 atcccttata ctgaagaacc agatattaat attggagatg aaatcactca aaaactaggg
     5341 aagggggaaa taatattaaa agtaattgat atgtcatttt taccaggagg tacactcaat
     5401 gttggtacta atcaccctca catgcttaca ttaaaagtcg agaatctgac tgctaatgaa
     5461 cataaaccaa aacaatctag ccaaaataca tttaacattg gtaacatatc cggtacacag
     5521 gttcaactcg gtgagcataa caatcttatt gtaaatctca gcataactga acttgttgaa
     5581 aaagtttctc aatcacaaga tcctcaggca aaaaatttgc ttaaggattt actaaataat
     5641 agtacggtag ccagtctcgt tggtgctggt gcgtccgcct tgttaacaat gctttgagta
     5701 tatggcctgg caaagagcca ggccatataa ttcatttctc actcatccaa ttaggaggta
     5761 gtggcatttt tttccttaaa acctgcaaat gctcaatcaa aacattaacc tgctcttgat
     5821 ttgttgcaag gatttcatct gatagcgtgc tacgaataaa atcatgatgg tcaatccaca
     5881 gtaaaccacc atttgcaagg gcctgacggt actgagtggt aggcattgaa gcaatatcat
     5941 gaagtccgaa ttgatcaaaa taattgttta tttcatgcgt cagaatcggc attatttctt
     6001 cctattttat ctaatttatc taatttgtag tcatttttaa ccagtattcg gtcaaatcaa
     6061 gcgcgtgttt ttgtgtgctt tactgtcata gtctttatct cacagcagta aattaaaatt
     6121 tttgcgtttt aacccttcac ctgttcacct tttgatattt tatcttttaa ttcataaggt
     6181 taaggggtga acagtttcac aaaaactatt caccaactgt tcaccactgt tcacccttga
     6241 agctcaataa acaatcaaaa aggtgaac
//
LOCUS       DAZFFG010000197         6263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFFG010000197 DAZFFG010000000
VERSION     DAZFFG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.198"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(178..552)
                     /gene="potE"
                     /locus_tag="ACHJ7J_004734"
                     /pseudo
     CDS             complement(178..552)
                     /gene="potE"
                     /locus_tag="ACHJ7J_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245180.1"
                     /note="catalyzes the uptake of putrescine via a proton
                     symport mechanism, as well as the efflux of putrescine by
                     a putrescine/ornithine antiport system; frameshifted;
                     internal stop; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
     gene            930..1286
                     /locus_tag="ACHJ7J_004735"
     CDS             930..1286
                     /locus_tag="ACHJ7J_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5002847.1"
                     /translation="MVKLASQPGASVARIAREHDINDNLLFKWLRLWQNEGRISRRLP
                     VTTSSDTGVELLPVEITPDEPKEPVTTLTPSLSTQTTVSANSCKVEFRHGNMTLENPS
                     PELLTVLIRELTGRGR"
     gene            1283..1447
                     /gene="tnpB"
                     /locus_tag="ACHJ7J_004736"
                     /pseudo
     CDS             1283..1447
                     /gene="tnpB"
                     /locus_tag="ACHJ7J_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1725..3262
                     /locus_tag="ACHJ7J_004737"
                     /pseudo
     CDS             1725..3262
                     /locus_tag="ACHJ7J_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611652.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3750..4490
                     /locus_tag="ACHJ7J_004738"
     CDS             3750..4490
                     /locus_tag="ACHJ7J_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO5002848.1"
                     /translation="MKKVFAVSVVAAACVFAANAGAEEGKSGFYLTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            4643..6142
                     /locus_tag="ACHJ7J_004739"
     CDS             4643..6142
                     /locus_tag="ACHJ7J_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5002849.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSVIITIACGYTEKNATGNVL
                     LLFLLLILAHRNTLTSITALLFLFCCALYAPVGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTRHRGHRNWLALLLFVLCSVNSWPLRMVKVTVVG
                     TTDTLREMQRYEQLSQHGADRWKILPGTPLYDTIVIVTGESVLRDYMSVYGYPVPTTP
                     WLNMAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADTSTPKVIFL
                     HMMGSHPNPCDRLHSWPNHYLEQYPRKVACYLASISKLDNFLGQLDGIFRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSLSRKISARHFTGIFQ
                     WLTGIRTENIPPFNPLTDEDNERIMVFNGERYVQEDSLKPQPLILPDHR"
BASE COUNT         1719 a         1408 c         1479 g         1657 t
ORIGIN      
        1 gatatattat gcgtgtgttt tatacaggtt attgttatta cccctatttc ctgcaaaaca
       61 acatattaat cactttcccc attatctgac tctgttccag gaaaccattc agcacattgc
      121 aggagaacac tgtctgcggg gaaatgggag acagaatctt acagactact ggcaacttta
      181 aatctgtttt ttcatttcaa aacatgggga taccagacca tacagtgtcc agccaggaaa
      241 agtaacaata aagcgctaca gcatggcttc ttctccggaa gagtagagcg caaagaagct
      301 gtacatcacg ccaataaagg aaacagcctg cactttttcg catcaaccgt attctgaaca
      361 atcaccagtg ccgccctcaa cagaatatac aggatgatga tttggctacc atggtcaggt
      421 tagccagcgc gttacgatga ctgtgctgag acatacttat ggtcatcagt aataatccgc
      481 tcaggaaaat cattacagcc aacatcccat gaacgggtat atatgcttag tcagacgaga
      541 gaatatttgg ggaagtatcc ataagcggga gtataagggc cggatacagg agaaagaaac
      601 gagcagcact gatgttcccg ggttttactg taatgctgga aaacctgcat aaagctgtat
      661 caggaaaact gcacaacaat agccagcagt cctcagataa acggaatagc aacccaaaaa
      721 caatgcaaga agattacact gcattcacgt caggacccaa tgcaggagag cccattttat
      781 gtaagcgtaa actgaccgcc gtatgtagcc attaagccag tattggtaac gtaggtgccc
      841 accttttcaa ctagtagaca cctgttatgg aacagaaaat attatcctca gaaccccgca
      901 gatcattttc aaatgagttt aaacttcaaa tggttaaact ggcttcacaa ccaggagcct
      961 ctgttgcccg tattgcccgg gagcacgata tcaatgataa cctgctgttc aaatggctca
     1021 ggctctggca gaacgaaggg cgtatatcgc ggcgtcttcc ggtaacaacc tcttctgaca
     1081 ctggcgttga attattacct gtggagataa cgccggatga gccgaaagaa ccggtgacta
     1141 ctcttactcc gtctttatcc actcagacta ctgtcagtgc caactcctgc aaggtggagt
     1201 tccgtcacgg taacatgacg ctggaaaatc cttcaccaga gctgctcaca gtgttgatcc
     1261 gtgaactgac cgggagggga cgatgatctc actcccgtca ggcacccgca tctggctcgt
     1321 tgctgggata accgatatgc gtaagtcttt caacgggctg ggtgaacagg tgcagcatgt
     1381 gctgaatgat aatcccttct caggtcacct gtttatcttt cgtggccgac ggggtgacac
     1441 tgttaaaatg taatgaacgc atcccacaca attaaacacg gagcagtatg atggttaaaa
     1501 gggtgaaact ggcaggtgtg gagcctcgca gatttccggc atcatagtgc ccaatacggg
     1561 atgcagaaag ctccctcaga ctggagactc cacaaatgaa atatacaccg gttggcgttg
     1621 atatcgcaaa acatctgatt caggtgcgta ttaccgtgaa ggagataggt gagtaacccc
     1681 ctgtgtcagc atagttgtcg cctcaatttt ttaaggagat aacaatgcca ggacaaccaa
     1741 taccaagtga gcttaaagag aaaggactga tatggctttt accaccatac tgctggtcac
     1801 atcgtcagat agcccgtaag ctgaagatca gtgcctcagt tgtatcaaaa tggagatatg
     1861 agttagtgga gcaagggctt ttacctgaaa atgaaaagat tacctccgtt gatattgaag
     1921 actggacacc ggagcgtcga ttctcagttg ttcttgagac ggctacgctg tcagaaatgg
     1981 agcttgctga ttattgccga cgcatgggat tgtttgttga acaggttaaa gaatggcggg
     2041 ctatctcaat tcaggcgcat gaaaaaaaat tgacggaaaa tattcgaaca gacagggcat
     2101 tgcgtgacgc aaaaaataag ttacgagaac tggagaaaga gcttaagcgc aaagataaag
     2161 ctctggcgga agctgcggcc ctgcttatat tgagggaaaa gttcaatgcc ctctgggaca
     2221 acagtgagga agactgcccc ctctcctgga gcggcgtaaa attattgccc ttgtccgcga
     2281 ggccgtctcc aacggcgccc ggaaaagtca ggcttgtagg tgtatcggta tttctattcg
     2341 gacacttcag cgctggactg atggcaacga tatcaccacg gatcgaagat ccgatactgt
     2401 aaacagatct ccccgaaata agctgtcaga agcggaaaga ttgcagatac tagagttgtg
     2461 caactctcca gagtttgcat ccttgtctcc aaatgtaatc gtgccaactc tggcagacag
     2521 agggacttat attgcttctg aatcaacctt ttatcgtgta ctgaaatcag ccaaccagtt
     2581 gacgaaaaga tcccgtgaat gccagtataa acggcctgat gtactcaggg ccgttgcgcc
     2641 gaatcaagtc tggagttgga acttcagcta tttgccgtcc tgggtcagag gccagcattt
     2701 ttacctctat atgatcgtcg atatttatag ccgaaaaatt attgctgccg aagtatttac
     2761 gaaagaatct ggacagtatg cggcagaact gcttcaacgt gccgtatgga atgaaaaatg
     2821 ttcatcccgc aatctcgtgc ttcattctga taatggagga ccaatgcgca gttgtatgtt
     2881 actgtcaaaa atgtacacgc ttggcgtatt aagttcttat tcccgtcccc gggtcagcaa
     2941 tgataaccct tattccgaat cgctcttccg gacgattaag tattgtccat ggtggccgga
     3001 gaatggtttt cgaaccatag atgaagccag aagttggtta agcaagttca cgcagtggta
     3061 caacctgaaa cacaagcact gcggtattaa gtatgttact ccggatgaac gctatcgcgg
     3121 aattgatgca caaatactgg aagccagaaa aaaagtttat cgcgaagctc gcaagcgtca
     3181 tcctgagcgc tggagcaaac agttgcgtga ctgggaactc attcaggcag tttatctgaa
     3241 tccagaaaaa gaagcggcat aacgtaattt tattacatca ggcgacaact accttgaaaa
     3301 acgccggaaa taactgactc tttactcgct gccatgcagt gggctttttt atatcccccc
     3361 ttcacaaact ctgctcactg gttataccgg ttcacctttc cggcttgtaa ccgcatgccc
     3421 tctggactgc cagtcagctc atgtatgtct ctttcttaat tgtgttcatg aacaggtgac
     3481 accttatctc ctgacagaat aaacaaattt tattaattct gctcttcgcc agcccactgc
     3541 caccatctgt cgcgacgggg catggctgcc tgcatggcat gcatttgttg tgtctgtgct
     3601 ggctgaacac gataaaaatt tccggttaat gacgatcgat atcatcgatc gattcgatcg
     3661 aaattattag attgatacta tctgttaccc gcttttaaaa tattcgaatt actatttgta
     3721 gcaacggagc aatagcggag ataattgaaa tgaaaaaggt ttttgctgtt tcagtggtgg
     3781 ccgcagcctg tgtatttgca gcaaatgcag gtgctgagga aggtaaaagc ggtttttatc
     3841 taaccggtaa agcaggtgct tctgttatgt cacttgcaga ccagcgtttc ctgtcgggtg
     3901 atggagagga aacatcaaaa tataaaggcg gtgatggcca tgatacggta ttcagtggcg
     3961 gtatcgcggc cggttatgat ttttacccgc agttcagtat tccggttcgt acggaactgg
     4021 agttttacgc tcgtggaaaa gctgattcga agtataacgt agataaagac agctggtcag
     4081 gcggttactg gcgtgatgac ctgaagaatg aggtgtcagt caacacactg atgctgaatg
     4141 cgtactatga cttccggaat gacagtgcat tcacaccatg ggtatctgca gggattggct
     4201 acgccagaat tcaccagaaa acaaccggta tcagtacctg ggattatggg tacggaagca
     4261 gtggtcgcga atcgttgtca cgttcaggct ctgctgacaa cttcgcatgg agccttggcg
     4321 cgggtgttcg ctatgacgta accccggata tcgctctgga cctcagctat cgctatcttg
     4381 atgcaggtga cagcagtgtg agttacaagg acgagtgggg cgataaatat aaatcagaag
     4441 ttgatgttaa aagtcatgac atcatgcttg gtgtgactta taacttctga caaaactgct
     4501 cctgaaagat aataattacc catcttctgt aattaaacga agactccctg cagtaattat
     4561 actgcagggt tatctgtttc acagataatt tctgattttt attatcttgt tctcttcata
     4621 acaatgttgt cgatatactc cgatgaatat cagaaaactg ttttgtccgg gcaacacacc
     4681 ccggatttta ttgtttttat tcttttttgt tgtttctgtc ataatcacaa ttgcatgcgg
     4741 atacactgag aagaatgcca ccgggaatgt gttgctgctg tttctccttc tgattcttgc
     4801 acacagaaat acccttacat ccattacagc tctgttattt ctgttctgtt gtgcactgta
     4861 tgcacctgtc ggtatgacgt acggaaaaat caacaacagt tttattgtcg cgttgttgca
     4921 gaccacggcg gatgaggctg cggagtttac cgggatgatt cctgtttatc attttctggt
     4981 cagtgccgcg attctggtat tcatggtgat tttctggcgg acacgccacc gcggtcatcg
     5041 taactggctg gcactactgc tattcgtatt atgctctgta aacagctggc cgttgcggat
     5101 ggttaaagta actgttgtgg ggacaactga cacattacgt gaaatgcagc gatatgaaca
     5161 actgagtcag cacggtgctg acagatggaa aatcctgccg ggaacaccgt tatatgacac
     5221 aattgtgatt gtcactggtg agagtgtgct cagggattat atgtcggtat atggttatcc
     5281 cgtgccaacc acaccgtggc tgaatatggc tcccggttta tttattgacg gttatacatc
     5341 ggcagcagcc agtactgtgc cttccctgag ccggacactg atttatgact atgagcagaa
     5401 ccctgattcc ggcaacaacg tggtggcgct ggcagcaaaa gcaggataca gcacatggtg
     5461 gatatccaat cagggaaaac tgggggagca tgatacacgc atctctgtta ttgcttctga
     5521 tgcggagcat accgttttcc tcaagaaagg cagctttgct tcccgtaaaa cggatgacat
     5581 gttgttgtta caggaaacag aacgtgcgct ggcagataca tccactccga aggtgatttt
     5641 cctgcacatg atggggtctc atccgaaccc gtgtgaccgg cttcactcct ggccgaatca
     5701 ttacctggag cagtatcccc gaaaggttgc ctgttacctc gccagcatca gtaaactgga
     5761 taactttctc ggtcagcttg atggtatctt tcgccggcat tcccgtcatt ttgccatgct
     5821 ttacttttct gaccatgggc tgtcggtcag cgatagtgct aatcctgttc atcatgatgg
     5881 tcatgtgcag ggaggctaca gcgtgcccct gattattacc gccagtgata taacctctca
     5941 tcagtccctc agcagaaaaa tcagtgcccg tcatttcaca ggtatttttc agtggctgac
     6001 cggtattcgt accgaaaata taccgccatt caatccactg acagacgaag ataatgaacg
     6061 cattatggtt tttaacggag agaggtatgt acaggaagac agtctgaaac cgcagccgct
     6121 tattcttccg gaccacaggt aatcatatat ggcaaactgt aatatagttg cttgtactga
     6181 aaagtattct gtataaaaca gaatccatat tcatatcagc acaagggacc accaaatccc
     6241 ggcagtattc cttacgtcag tta
//
LOCUS       DAZFFG010000198         6259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFFG010000198 DAZFFG010000000
VERSION     DAZFFG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.199"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            29..853
                     /gene="rcnA"
                     /locus_tag="ACHJ7J_004740"
     CDS             29..853
                     /gene="rcnA"
                     /locus_tag="ACHJ7J_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO5002850.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTTKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1071..1409
                     /gene="rcnB"
                     /locus_tag="ACHJ7J_004741"
     CDS             1071..1409
                     /gene="rcnB"
                     /locus_tag="ACHJ7J_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO5002851.1"
                     /translation="MTIKYKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1491..2525)
                     /locus_tag="ACHJ7J_004742"
     CDS             complement(1491..2525)
                     /locus_tag="ACHJ7J_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO5002852.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYGELSAQHNLVVQGDFALTQNQ
                     TATHTYTFNDTSCVSTNTVAQIDPSDIVIGLYNDTIKLKLHFEWEKKGSITLSNNQTS
                     FTSGYNVTVTSAASDAIVNLSGGGSGSLMINSVATLSSASSSTRLAAFLQFAGCFLGG
                     TSWDICVQNYKNALSKNAGVYSFNLALSYNPITTTCKPDDLLITLESIPILQLPTTGN
                     KATINSKQGDIILRCKNLLGQQNQTSRRMQVYLSSSDLLAGSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(2539..5019)
                     /gene="yehB"
                     /locus_tag="ACHJ7J_004743"
     CDS             complement(2539..5019)
                     /gene="yehB"
                     /locus_tag="ACHJ7J_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5002853.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGAWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(5035..5709)
                     /gene="yehC"
                     /locus_tag="ACHJ7J_004744"
     CDS             complement(5035..5709)
                     /gene="yehC"
                     /locus_tag="ACHJ7J_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5002854.1"
                     /translation="MKGLSSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(5789..>6259)
                     /locus_tag="ACHJ7J_004745"
     CDS             complement(5789..>6259)
                     /locus_tag="ACHJ7J_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002855.1"
                     /translation="VDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVPTTVFEGKAKY
                     STYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTVGYYLYLGDN
                     SEVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDSSVNSGDVLSSLVMRVAQD"
BASE COUNT         1703 a         1496 c         1233 g         1827 t
ORIGIN      
        1 tgccgaatta atactaagaa ttattatcat gaccgaattt acaactcttc ttcagcaagg
       61 aaacgcctgg ttcttcatcc ccagcgccat cttacttggt gcgcttcatg gcctggaacc
      121 agggcactca aaaacgatga tggcggcgtt tatcatcgcc atcaaaggca ccactaaaca
      181 ggcggtgatg ctcggactgg cagcaaccat ttcgcatacc gcggtggtct ggttgattgc
      241 ctttggcggg atggtgatca gcaagcgctt tactgctcaa tcagcagaac cgtggctcca
      301 gttgatttcc gcagtgatca ttattggcac cgcgttctgg atgttctggc gtacctggcg
      361 cggcgaacgc aactggctgg agaatatgca cgagcatgat catgagcatc atcaccacga
      421 tcacgaagat caccacgacc atggacatca tcaccatcac gaacatggcg agtatcagga
      481 tgcccatgca cgagcccatg ccaatgacat taaacgacgc tttgatggta gagaggtcac
      541 caactggcaa attttgctat ttggcttaac cggtggcctt atcccctgcc cggcagcaat
      601 taccgtgctg ttgatttgca ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt
      661 cagtttcagc attggcctgg cgttaacgct ggtcaccgta ggcgttggcg cagcaatcag
      721 cgttcagcag gtcgcaaaac gctggagcgg atttaacact ctcgctaaac gcgcccccta
      781 tttttccagt ctgttgattg gcttagtcgg ggtgtatatg ggagtgcatg gctttatggg
      841 tattatgaga taaaacaatc acctgggctt cgctatcctt ttagcccagg atgattctta
      901 aatcagctta ttccgctcac ttctcgctca atgaggatgc ttttgctaca tcctttcatt
      961 tacacttttt acgaaatcac gggatcacta acaaaatatc tcttgtcagt tatattgtat
     1021 ggcatgaaag atatgcgact gatattacag atccccaaag tggagaattt atgaccatta
     1081 aatataagat gttgctgggt gcgcttttgc tggttaccag tgccgcctgg gccgcaccag
     1141 ccaccgcggg ttcgaccaat acctcgggaa tttctaagta tgagttaagc agtttcattg
     1201 ctgactttaa gcatttcaaa ccaggggaca ccgtaccaga aatgtaccgt accgatgagt
     1261 acaacattaa gcagtggcag ttgcgtaacc tgcccgcacc tgacgccgga acccactgga
     1321 cctatatggg tggcgcgtac gtgttgatca gcgacaccga cggtaaaatc attaaagcct
     1381 acgacggtga gattttttat catcgctaaa aaaagccccc tcatcatgag ggggaaatgc
     1441 agacaccttg ttatttttta ttattagcca cttgctcgtc ttgcttgtta ttagtcgtat
     1501 ttcacgttga ttaatgcggt tgcctccagt gcgccagatt taattttatt tgtatcgtag
     1561 acgtagtaac tggcagttat cggaattgaa accgttgtat ctgtaagttt tgactttgcc
     1621 gcaatttctt ttaaatttgt gtatccttta ctgctgttag tgatatttaa aagtgtgact
     1681 ggcgaaccat tactttcaag aataaatccg actccattat cttctgcacc tttcaggatc
     1741 gtgttactgc cggctaacaa atcagaactt gataaataca cctgcattct ccgtgatgtt
     1801 tgattctgct gacctaataa atttttacag cgcagaataa tatccccttg tttactattt
     1861 attgttgctt tgttacctgt ggttggtaat tgtaaaattg gaatactctc taaagtaatt
     1921 aataaatcat caggtttgca ggtcgttgtt atcgggttat atgacaatgc cagattaaag
     1981 gaatagacac ctgcattttt tgataatgca tttttatagt tttgcacaca gatatcccat
     2041 gaggtgccgc ctagaaaaca acctgcaaat tgtaaaaaag cagccaaacg agtagatgat
     2101 gaagcactgc ttaatgtcgc gacactgtta atcatgagag atccacttcc cccaccagac
     2161 aaattcacta tggcatcgct cgctgccgat gtcacagtga cattatatcc actcgtaaaa
     2221 ctagtttggt tatttgaaag cgtgatgcta ccttttttct cccattcaaa atgcaactta
     2281 agtttgatgg tgtcattata gagtccaatc acaatatccg aggggtctat ctgcgctact
     2341 gtattagtac tgacgcaaga agtatcatta aaagtatatg tatgagttgc tgtttgattt
     2401 tgagttagcg caaaatcccc ctgaacaacc aaattatgct gtgctgacaa ctcgccataa
     2461 catgcagcat aactcacagg cataaccatc atcattacta taaataacat tatacgaata
     2521 ttcataacca cctgactatt actggcaaat ataattccta ctttcatcaa tctctttacc
     2581 gaaggtgatt gtgcatgaaa gtccttgttg cttatcaatt gctacattaa ccgatggcgg
     2641 tacttcattg gtgcgaataa ataactgact tccctggccg acaacgccaa tattatgacc
     2701 atggatatca ttgacttcat aaccaaacgt taatggttgc ccatccgctc ttaacgcttt
     2761 tataaaccag ggcttgcgct gatcggtatc aaaattaacc agtacaaccg cgccgcgata
     2821 agaggcggca attttccggt taccgcgcaa ttctgcttcg ctatcgcttt gcgacacatc
     2881 cagcatgagg tgattttccc gataaggtgt cattccgtcg tataccacca ctccattacg
     2941 gtttgttgtg cgatattttt gcccattgac ataagcatct ttaattcctg gcgcattcat
     3001 cacagcaaac gtttcggaaa gacggttcgc cagattaacg ccacccgacc aggcgacaat
     3061 gccccctgaa acactggctc cagcctgtcg ataagtactc gactgactat aactgccatt
     3121 cactgtcgca accggcgcgt tccaggttaa attcgctcca gctgtcgttt catttccctg
     3181 atgttgatga ctcaggttga caccataatt gaactgatcc cgactcccta ctgttcctga
     3241 taatcccgta ttatttgagg caaacccctg atcatcaaac gtcgttgagt tagacatata
     3301 tatttgccga cgaggcgtcg aaacgtcatc accccaatca aagggaatcg atataaaaat
     3361 attaaaacgt ttctcttcat gatgattctc gtcataagcc tggcttgccg cgagggtata
     3421 gcttatccgt cgcaggttgt tggaataact caactgataa tccttactac tgccgctacg
     3481 cccccagtaa tctcgccata acgtacttaa tgacacagaa ccccaacctt ctggcaatga
     3541 ctggctcata ttggcggaaa agctattttt gcgaccaaaa tcattctggt aataatcggc
     3601 aatgtcatag acatcgtttt tatcacggcg ataattatct ttattatttg cccacacata
     3661 atcgttaaat gtgcggtaat cacgcgacga ataacgccag gccgccagac caaaacgtgt
     3721 tgatgtttgg ctcaaaaatt tgttataggc aatttgataa ctttgtccgt caaatacatc
     3781 gccgttgtct tgtttactat gcgacttcgt ggcatcgacg gaaatggcac caatgcgtgt
     3841 attccagcca gtccccaaag tgaacgcgta ataattattc gcgaccatcg agccaccata
     3901 cagcgtcaat aagttattaa aaccatactg ataacccgcc tggacaaaat cactttgttt
     3961 gctcgcccct tcaatatggc tacgacccgc cgcaaaatca tatttcgaca cgccgggttg
     4021 cagcatattt ggcaccgctg cataaggcac cagataggtg gttaccgaac cgtccgcctc
     4081 tttcacgctg acatcaagat ctgctccacc accggccaac tgcaaatctg taatcgcaaa
     4141 cgggccaggg ggaacctctt tctgataaac cacaaaaccg ttctgttcaa tagttaccag
     4201 cgcgttactc tgagcaatcc cctgcacccg tggcgtaaaa ttttgtttcg agttaggcaa
     4261 catctgcata tcacgaaaca accgcacacc tctgaagcga acagaatcaa aaatatcgct
     4321 tgatgtgtac atatcaccca cgcgaagcgt gccgagaagt tgggcaaatc cacgttccag
     4381 atacagggta ttgcttttcc acgcccctgg attgttattt gttttactga aactggcatc
     4441 agaatgcagt tgccacccca gtaaatttaa cccgctgtta aaacgtacat atgtactctt
     4501 actattaccc gacgctttat agtcactgta atactgactc acataataag aggtataaaa
     4561 cgcattaata ccccgctccc agttttccgg tggaacatag ccactttcca gttcttccac
     4621 ccaggcctgc gggacactga aatcgagacg aaaaaccccg atatcccagg tatagctccc
     4681 accctgaaca agttgctcaa atgttaaaca ttgcttaccg ctggcgaagt tatcgctatt
     4741 aatgcctaac cgcttgataa tttcccttga taaacatgtt tcttgcgggt tgtctttaac
     4801 aatgatctca tatttcccgc gccattgctt attgacataa atatcgatgt catactgccc
     4861 cggtaagggt tgattatcat caagacgaat atttgcaacc tgctggtctt tcattccacc
     4921 tatcataaaa tgggtatcaa aggtttcttc agctgcataa gcttcaatgc cgagcaataa
     4981 cgctacgatt gctgatgcaa gtggggtcat tctcaacata ggcagctcct gcaattaaat
     5041 tttgtcacta atatagttgc catggtcatc gataatggtc agatgccagt tatttgcatt
     5101 attacttttg acattaacac tctgactttc taagggggca atcataatag tttcataatt
     5161 gactttgaca ttattagctt tgacatccga aatcgtcacc caattagctg agtcattttt
     5221 tatcaccaat ccattgccac tgcgatttac cagtaacttt ttaaaggtcg ctttatttac
     5281 cggagcaata cccgccggcc ggtaaaacaa tttgattctg ttttgcatcg caaacttcag
     5341 tgcattctta ccttcttgtt ctggactatt tggtggaata tccagaacat tcagataaaa
     5401 aatgctttct ttattagtgg gcagtttatt cggcataatt ttgattttta cttgctgccc
     5461 ggaatttgcc cctatttttg ccactggtgg cgttaacatg aaaggaacct gaattttttc
     5521 tggtggtaat gacgtatcgc catcatcaat ccacgcctgc agcagcgaag aacggtttcc
     5581 ctggttcatc aactgcacca tcacttcttt attttctgcc gggtaaataa tgcgcgtccc
     5641 gtagataaca attccggcat gtgcaggaag gaccatagaa aaaatgagta aagatgataa
     5701 tcctttcatt ttaatgcctc ttaaattaaa aggccgccat gggatagcgg ccatgtttat
     5761 tacctggaga aacttacaat tttatttatt aatcctgcgc cacacgcata accagagaag
     5821 ataacacatc acctgaattc actgagctat ctgtcagttt caggtatttt gctttaaacg
     5881 gaatagcata ttgaccttcc gctgttttat aactctcaat attaaaaggt gcagaaacat
     5941 ccaggacttc gctgttatca cctaaataga gatagtaacc cacagtatcg ttgctactgg
     6001 ctatcgcctt atcgttacca gttattttat cgttggtaat caacgagagt tttacaccag
     6061 caacttcttt cgggcagcta atttttaaca tgctgctggt tacaccgact gcatcatcat
     6121 aggtagaata tttagcttta ccttcaaaga cagtggttgg tacagttggc atattaatac
     6181 ttactgaatc accacccgct tcgaatttac acggagattc tgcaatattt cctttaatgg
     6241 ttaatgttcc ggtatcaac
//
LOCUS       DAZFFG010000199         6064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFFG010000199 DAZFFG010000000
VERSION     DAZFFG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.200"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1051
                     /locus_tag="ACHJ7J_004746"
     CDS             <1..1051
                     /locus_tag="ACHJ7J_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO5002856.1"
                     /translation="RNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEA
                     KPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLK
                     DFKQGPTGKESLVYGIYGISESNLSECQKGIKRVVALTPALQPIDGVAVSYIDAAVAL
                     GNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQL
                     AELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKE
                     ADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRA
                     LREYNEMVDDYNSLR"
     gene            complement(1101..2849)
                     /locus_tag="ACHJ7J_004747"
     CDS             complement(1101..2849)
                     /locus_tag="ACHJ7J_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO5002857.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQSVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(2849..3919)
                     /locus_tag="ACHJ7J_004748"
     CDS             complement(2849..3919)
                     /locus_tag="ACHJ7J_004748"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5002858.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            complement(3909..5360)
                     /locus_tag="ACHJ7J_004749"
     CDS             complement(3909..5360)
                     /locus_tag="ACHJ7J_004749"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO5002859.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5371..5817)
                     /locus_tag="ACHJ7J_004750"
     CDS             complement(5371..5817)
                     /locus_tag="ACHJ7J_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO5002860.1"
                     /translation="MAALTASCIDLNIQGNGTYSILKQLATMALQNGFITDLHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
BASE COUNT         1581 a         1520 c         1532 g         1431 t
ORIGIN      
        1 aaggaattta ttatcttccg ctattatagt cgccatcatg gccctcggtc tcacgggttg
       61 tgatgataaa aaagccgaaa cagaaacgcc cccgcctgcc aatagtcaac ctgccgcacc
      121 agctcctgaa gcgaaaccta ctgaagctcc cgttgcaaaa gcagaagcta aacctgaaac
      181 acctgcgcaa ccggtggtcg atgaacaagc ggttttcgac gaaaaaatgg atgtctatat
      241 caagtgctac aacaagttac agatccctgt acagaacagt ctggcgcgtt atgctgactg
      301 gctgaaagat tttaaacagg ggccaacggg taaagagagc cttgtttatg gcatctacgg
      361 cattagtgaa tccaatctct ctgagtgtca aaaaggaata aaaagagtcg tggcgttaac
      421 gcctgcgctg caacctattg atggcgtggc ggtgagttat attgatgctg ccgtggcgtt
      481 gggtaatacc attaacgaaa tggataaata ttacacccag gaaaattata aagacgatgc
      541 gtttgcgaaa gggaaaacgc tgcaccagac attcttaaaa aatctggaag cctttgaacc
      601 tgtagcagaa tcttatcatg cggcgattca ggaaattaat gataagcgcc agcttgcgga
      661 actgaaaaat attgaagaaa gagaagggaa aacattccac tactactctc tggcagtcat
      721 gatttcagcg aaacaaatta ataacctgat atcgcaagag aagtttgatg ttgatgcggc
      781 aatgaagaaa gtctctgaac tggaaacgct ggtagcgcag gccaaagaag cggataaagg
      841 cggtatgaat ttctcgttca ttaattcggc agaccagtat caactcgaga ctaaaaaata
      901 tgttcgccgc gtcagagata aagtcccgta ctctgactgg gataaagagc atcttcagga
      961 tgcaaacaca agctggatgg tcgaagactc atttccacga gcgttacgcg agtacaacga
     1021 aatggttgat gactataata gcctgcgtta aacgtttttg atgataaatg cagtgtcgga
     1081 tgcggcgcat ccgacacagt tcaccccttg tgaagtaact ccataaatgc ctcatatcct
     1141 gttaacccgg caataacttc tgccggatgc tgacacaata cgcttgccag atagctgaat
     1201 atttttagct catgacgcgc atcggcggcg gcgcaggcga tcaagacatg gttaatgact
     1261 tcgttattca cctcaactga ttgggcgagg gtaataaaaa atccacgaaa acgtcgctcc
     1321 tgttcgctcc agcaatgtgg gatggcgagg cgattaacaa tcaggttttc accttcgcct
     1381 tcgcgggcga tgatccgttg cgcttcatcg gcggtaatat ggtgttgtgc gactaattgc
     1441 gcacaaattt gccgggtaat atgttgccag ctttcaccgc gtacattcga ataatgaaaa
     1501 cttccggggg cagagaaaaa acgctccggt tgctggcgaa taaatgccgt cgccagaaaa
     1561 tgttttattt gttcgatacc ggcagccgta atgatatttt ttacggtgat gtaattattt
     1621 accgcgtcat ccagtaaata atggctgttg ttaatgatca ataacggctc aatctcttcg
     1681 cgaatggcaa caagctcgct taagctacgg gcaataatca cccgacaatg taaaacatcg
     1741 cgctcaatgg cgagctgatt aatggtggca atcgcgttct ggtccgagag caaaatgatc
     1801 ggctggcgtt cgttttgatg tcgttccagc gcacaggcaa aatacaaacc gatcagatcg
     1861 ctgtcgaaaa gaggaatatc gagctgttct cgcagtagcg taataaagtg cagactcata
     1921 tcaaaggccg caggccaggc ggctttcagg ttattcatgc tgctttggcg gtgctcggca
     1981 atccacaccg gtgcggcaag gcaacgtaat aaatgcccgg taatattctc aaccaactgc
     2041 gtatcaataa tattcaatga cgcgatgcct ggcacccttt gcagcaaccc cagcacatga
     2101 tcggcagaaa ttacctgcgc ttgctgatgc tgtttctcta tcaaacctgt cagggttctt
     2161 acagcgttat caccaagaaa cagaccgcta tgttcaacga tttgttttat ctcgtcaccg
     2221 ggatattgcg gccactcatc ggtcagttga ttacgcaggg cgtaaatcgc cagaatcagg
     2281 ctggagagat aatcaccctg catgagcggc cagcggtgtt ggttgtcgca gaccgtggat
     2341 aaatgctgaa ggttatctcg tgttacgcca gccaaaggta ttaaaaacgg atctttgcgt
     2401 agcaagttcg ccagcaagat aacgcgtttc tcttctgttt catcaatgaa atggcccaaa
     2461 ccggggcgac tggccaggca gcaagtacgt tcataacgct gtttcaggcg gggcagacgc
     2521 tctgctaccc acgtttctgg caaattaagc gccgaggcga gttgcgcacg gggagtgaaa
     2581 gtattcagta ataacagcgc cagcagccga tcgtcgttat cgtgcttttg cagcaactga
     2641 aaaaaactgc ggcgctcgaa gatttccagc tgatagcccg cactgccact ggcagagata
     2701 cgagctttgc cgttaagggt aaagttgaga tagtcaatat cacgcaggat ggtcctgcct
     2761 gaaacgccag tctgttgcgc cagctcgcca ggcgtgcgcg gctgttgctc cagcagatcg
     2821 acaatcttta actggcgttc gtttagcatt aatccacctg acggaactgg ctaaacgcgt
     2881 tgactttctc cgcagttaag gtcgtcagaa aaccccgtat cagcgtgagc agagcatcat
     2941 aatcggcttt tgaaatcata ccgctgttgg cgtgcagata acgagttggc agacacagcg
     3001 cgacaaccgg acgcccgccg cccatcacgt tgtagcgccc gccatccgtc gctccggttt
     3061 tcatggtgga aaattgcagc ggtaaatcat tctgcgtggc acagtttttt aacgctgcta
     3121 ccagtttctg gttggggaag tagcgcttgt caaacagcat cagccccggc ccctggccca
     3181 gtttcagcgg gtatttaatg ttatcaatgc ccggaacatc gcccgcgacg gcggtatcca
     3241 gcacgatcac cacatccggt ttagtgtgtt cagccgaggt ttgtgccccg cgtagcccaa
     3301 cttcttcttc cacgctgccg acgccgtaca gcgtaatttc tgggttattt actgtctgta
     3361 gtagctccgc catcatcgcg cagccgatgc ggttatccag cgccttgccg actactttat
     3421 cttcgcccca gcaggcaaaa ttggcctccg ggctaataaa attaccaatt tccacgccgc
     3481 gttttttcgc ttcttcgcga ctgttcgcgc caatatcaat gaacatctca tcaaatgaca
     3541 gcggttgttg cttttgcttt tccgttaacg catgaggcgc tacggaacca atcacaccag
     3601 ggattttcac tcccttgtgt gtgcgtattg ttacccggtg gttgagcatc gactgattcc
     3661 accagccgcc aatggtggta aagcgcagaa aaccgctctc gtcgatgtgg gtgaccataa
     3721 agccgacttc atccatatgc ccgacaacgg caacttttgg acctttattt cccttacggg
     3781 caacaaagct gcccagacca tcaaaggtga tctcattaac gcaaggttcc agcgtgttta
     3841 tcagaatgtc gcgaacttcc tgttcatcgc cgctgacggc gctggcttcg cacaactgtt
     3901 gcagtaactc aatgttcatg gacaagctcc tgttgcgcgg ctttcgcttt gcggcgtaac
     3961 cacaatcctt tcaggacgat aatgaatgaa atgttcagcg cgaggccaat cgccagcacc
     4021 agataaaacg aacccaccgg cgacatcagg ccgatcagcg ggtcaaagat acccaaacca
     4081 ggtgccagac gtttgattcc gaaagcgatc accagcacgc cggtaatacc gcccgccagc
     4141 gtattggcgg taatcatcgg caacggagct gccagagcgt aaggaatagc cggttcggtt
     4201 gcgactgtgg caccgaccac aatggcacta ctggcagcct ctttttcctg tcgggtaaac
     4261 agtttgggcg caatgaacgt cgcaagaccc gccgcgacgg gcggcatcag cgcgacaacg
     4321 ccgacgatgg cgtaccagtc ataaatgtgt ttttccagca gtgagaagca gaagaaccag
     4381 gcggttttat tgatggggcc gcccatatcg aacgccagca tcgcaccgac cagaaacgct
     4441 gccgcaaact tcattgatgg aggaatggta ttgaggaagt gcagtagccc gcccatgagg
     4501 tcggacatca ccggtccgat aacgtagtaa gtgagcacgc cgaaaaccag cagagtaacg
     4561 aacgggatca gcattgaacc gagcagcggt tgtagcgctt tgccgagacg gactttacgg
     4621 aaccagaaga cgaaatagcc aatcgccagc cccagcacca ccgcgccgag gaatcccgca
     4681 cccgactggg tgccgagcag cgctttgtcg ttcgccagat agcaaaccag aaacgccgga
     4741 gcgaaagccg gtttatcggc aatcgacgag gcaatataag cgcccatgat gggaatcata
     4801 aaggtgaagc caaggtaacc gatggattca actacccagg taaacgacgg cgcgcctttc
     4861 gacatatcgg tatacggcaa accgaactgc accagcatgt tggcaagcgc caccaggata
     4921 ccaccgccga tgacaaacgg cagcgccgca gagacgcccg ccatcaggtg gctcatcacg
     4981 ctgccgcttt gtacttcctg tttgcctagc ttcacgccgc tatctgcggc aaaaagctgc
     5041 gaatttgtcg gtaattcact gaaaatctga tcgatatttt tcagcgcctg ggagatggca
     5101 atctcataaa cttttttccc ggcaaaccgt gcgcgatcgt caccgctcag gccacgcccg
     5161 gtggcgagaa tcacgtaatc ggcagcggca atctcttcgc tggataagcg gttttcaacg
     5221 ccgctggacc cttgagtttc cacttttatg gtatggccga gagaacgggc tttctgttcc
     5281 agggcttccg ccaccatgta ggtgtgagcg atcccggcgg ggcagttggt gatcgcgaca
     5341 atacgtaagg atgactccat cgccacttcc ttatgagctg agggtttgat ttaacaaggc
     5401 aagcacttgg tcggcgtcgc cctgttgcag ttggtgaata aaatcctggt gaataatttt
     5461 gcgacacagt gtgccgatga ttttgacctg atcctcttcg ccactttgcg gcacgccgag
     5521 gcagatccag caattgacgt cttcgccgtc gctggctttc cagtcaatag cctgcgcttt
     5581 gcgggcgaat aacacgaacg gttgtttaac gcaggcgctt ttaccgtgcg gcacggcgac
     5641 accagaacca aaaccggtgg agtgcatttt ttcgcgcagc agcagggttt gcaggaactg
     5701 gtgtaagtcg gtgataaaac cgttttgtaa cgccattgtc gctaactgtt tcagaatgga
     5761 ataagtgcca ttgccctgaa tattcaggtc aatacagctt gcagtaagag ctgccattaa
     5821 tcggactcca tgaaaaaata tttacgttaa ttgtaatagc ggggcgggtt tgtgatgagc
     5881 gcggatatgt cgtgaagtcg tgacaatata aagcagagtg aaggataaat tttgcggcgt
     5941 aaggcacata ttagtaggta aaataaatgc cgtgaataaa aaatcattct ttaatgattt
     6001 tatgaatgtt taactgccgc ggatattttg ctgcaacgtt atttatattt gtggcttatg
     6061 agtc
//
LOCUS       DAZFFG010000200         5832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFFG010000200 DAZFFG010000000
VERSION     DAZFFG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.201"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            441..1004
                     /locus_tag="ACHJ7J_004751"
     CDS             441..1004
                     /locus_tag="ACHJ7J_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5002861.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEIHRLLSETQSMLAGYYWVMEYTQNKGLHIHFIGYLDGQRHKKS
                     YRISRQLGDIWRRITEGDGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            1157..3517
                     /locus_tag="ACHJ7J_004752"
     CDS             1157..3517
                     /locus_tag="ACHJ7J_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5002862.1"
                     /translation="MVHKSDSDELAALRAENVRLVSLLEAHGIEWRRKPQSPVPRVSV
                     LSTNEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQLGICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTAGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVIPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNIAVTEPLIITLANQIYFEK
                     AQLPQALVNRLIRLTAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     ALSFLRYNNIAAELIDKRFAGTECNAVFTGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIARRKVNTLILVHRTELLKQWQERLAVFLQVGDSIGIIGGGKHKPCGNID
                     IAVVQSISRHGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAARPKESLHNLEVLTRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDDIASVMNTLKLSPFVLHSRLSKKKRTM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIVADGEGLSF"
     gene            complement(3535..4161)
                     /locus_tag="ACHJ7J_004753"
     CDS             complement(3535..4161)
                     /locus_tag="ACHJ7J_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002863.1"
                     /translation="MTLRLTLIDELIRKQHHYLEDGDLCYCFGEYTARQGAAYSKTNQ
                     LIINLKKGYERRAFPDYHYKKAAIEYVAQMLISNIGNLEEYTFVPVPPSKCRTDAAYD
                     DRMTEILRIAQTYEPWLDYRELVVQNVSTIASHTAANRPSPEAIMANYQLNEQLLEGC
                     RPWIVIFDDVLTAGSHFKAMKSLILKHIPDACILGLFVARTTRGAQII"
     gene            complement(4158..5051)
                     /locus_tag="ACHJ7J_004754"
     CDS             complement(4158..5051)
                     /locus_tag="ACHJ7J_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO5002864.1"
                     /translation="MSQFDFTSLDPFARAISPFEEMAAYEALWSEKGATFKTIADKFR
                     AAPTGSLPSDFVSDSIIAAFKNRLKEIIARYDIDDLGIRVHGAGEYPEKLRDARNPIE
                     LLYYQGWWDLINTRSVAVVGSRRVSEEGAKRTRKVVKFLVEDGFTIVSGLAEGVDTNA
                     HQTALALGGKTIAVIGTPLSHHYPRQNSNLQKNIRDNHLLISQVPFHRYLEQDYRSNR
                     IFFPERNVTMSALTEATIIIEASETSGTLTQARAALAQGRKLFILESCFRNPSITWPA
                     RFEAQGAIRVRNYEDIRRHLR"
     gene            5649..>5832
                     /locus_tag="ACHJ7J_004755"
     CDS             5649..>5832
                     /locus_tag="ACHJ7J_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO5002865.1"
                     /translation="MQHRQDNLLANRTSFPGMVSGQYAFRIRTLSQVVRYFSLLPCLC
                     ILSFSSPAAMLSPGDRS"
BASE COUNT         1491 a         1245 c         1419 g         1677 t
ORIGIN      
        1 agtttatggg taaatataat cttttctgta tgaaataatc ttcttattgt gagattaaat
       61 atctgtagac tctctctgca gagtctctgt ttttacttaa tggtacggca atactaatag
      121 cgatgttgcc tgtggctatt caatgctgac ttttgtgaag atcagattat ttcctgtaag
      181 taattgaatg gtgctttctt aaactcaatt atatgaggtg tgttttttat tgtaattcgt
      241 tctggtcctc agtttaagag agtcttactg tattctgaat cattacccta ttactgtaat
      301 gaggtaatac gaaacagttt tatcaggttg tctgtgctgc tggttacctc gtttattaat
      361 gccgcggtac ggcgtttctg tactctcagt atttatgtaa ttccactttc ctgtcagttt
      421 tataaagtaa ggactataaa atgtataatg ctaatcccaa ttacgaaatg gatttcatga
      481 ttctgaaaga cgtgaatgaa cacatggaag gtctgtttca acgtttttct aaactgttac
      541 ctttcagaat tgattttgcc tacaggaaag acacgcccag ttttggtcat tcctgtaggc
      601 acagtatgtg tattgaaata caccgcttac ttagcgaaac ccaatcgatg ttggcgggat
      661 attactgggt aatggaatat acacaaaaca agggacttca tattcatttt atcggttacc
      721 tggatgggca acgtcacaag aaatcttatc ggatatccag gcaactggga gatatctgga
      781 ggcggatcac ggaaggggat ggttattttc acctgtgccg ggctaaagat aaatacccgg
      841 tgcgtatcga tcacgtgatt cactatagtg ataagtctgc ggtagacgac ttacgttacg
      901 ccctcagcta cctggctaaa caagatcaga aagaacacgg tattattctg ggccgtagca
      961 ggcttcctga aaaaagtaac cgcggccgac cacgacacaa ctgaattaca gctgtattag
     1021 gtattagaat taagtttctg taagttgttg ggaggtgctt gttaataggc agaagagggc
     1081 ggggcgcttt gtcatcagtg taggggaccc gaatttctgc cgtggtgcct cagtggttcg
     1141 gatacaggta acgactatgg ttcataaatc tgacagtgat gaattagctg cattgagggc
     1201 agaaaatgtc cgtctggtct cattacttga agctcatggg attgaatggc gacgtaaacc
     1261 gcagagccct gtgccgcgcg tttctgtatt atccaccaat gagaaggttg cattatttcg
     1321 tcggttgttt cgcgggcgtg atgatgtatg ggcacttaga tgggaaagta aaaccagcgg
     1381 caaatcaggg tactctcctg cctgcgctaa cgaatggcag ttgggaatat gcggtaagcc
     1441 ccggataaaa tgtggggact gcgctcaccg tcagttgatt cctgtatccg atctcgtcat
     1501 ctaccatcat ctggccggta cccatactgc cgggatgtat ccgttgctgg aagatgattc
     1561 ctgttatttt ctggcagttg attttgatga agctgaatgg cagaaggatg catccgcatt
     1621 catgcgatcc tgtgatgagc tgggtgtacc tgctgcgctg gaaatatccc gttcacgtca
     1681 gggggcgcat gtctggatat tttttgcctc acgagtttcg gcccgcgaag ctcgccgtct
     1741 ggggactgct attattagct atacgtgtag tcggacccga cagctgcgat tggggtctta
     1801 tgaccgatta tttcctaatc aggatactat gccaaaaggg ggatttggta atctcatagc
     1861 gttacctctg caaaaaagac cgcgtgaatt ggggggaagc gtttttgttg atatgaatct
     1921 ccagccttat cctgatcagt gggcttttct tgtatcggtg atcccgatga atgtgcagga
     1981 tattgaaccg acgatattac gggctacagg gagtatccat cctctggatg tgaattttat
     2041 caacgaagaa gacctgggta cgccgtggga agagaaaaaa tcatcaggaa acagactgaa
     2101 tattgctgtt actgagccgc tgataatcac gctggcaaac cagatctatt tcgaaaaagc
     2161 gcaattacct caggccctgg tcaaccgact gattcggctg acagcatttc cgaatcctga
     2221 gttttataag gctcaggcca tgcgtatgtc agtctggaat aagccccgtg ttataggttg
     2281 tgcggagaat tacccgcaac atattgcgtt gccccgggga tgtctggaca gtgcattatc
     2341 tttccttagg tacaacaata ttgctgcaga attaatcgat aaacgatttg ccgggacgga
     2401 atgtaatgcc gtttttacgg gaaacctcag agcggagcag gaagaggccg tttcggcatt
     2461 actccgttat gacactggtg tactttgtgc gccaacggct tttggtaaga cagttaccgc
     2521 agcggcagtg attgccagac ggaaagtgaa tacactgata ctggtacacc ggactgaatt
     2581 gctgaagcag tggcaggagc gtctcgcggt gtttctgcag gtcggtgaca gtattggtat
     2641 tatcggggga ggtaaacata aaccctgtgg caatattgat attgcggtgg tgcagtccat
     2701 atccagacac ggagaagttg aacctctggt cagaaattat gggcaaatca ttgtggatga
     2761 gtgccatcat attggtgcgg tttcattttc tgcgattctg aaggaaacga atgccagata
     2821 tctgcttggc ctgacggcaa caccaatccg acgggatggt ctgcacccca ttatttttat
     2881 gtactgtggt gccattcgcc atactgcggc ccgcccgaag gaaagcctac ataatctgga
     2941 agtgctgacc cgttcccgtt ttacatctgg tcatttacca tcggatgcga gaatccagga
     3001 tattttcaga gaaattgctc tggatcatga cagaactgtg gcgatagctg aagaagccat
     3061 gaaagctttt gggcaggggc gtaaagttct ggtactgact gaacgtacag atcatctgga
     3121 tgatatagcc tcagtgatga acacactgaa attgtctccc tttgttctcc atagtcgatt
     3181 atcgaaaaaa aagcgtacta tgctgatttc cgggctgaat gctcttcctc ctgattctcc
     3241 ccgtattttg ttgtcaacag gcagacttat tggtgaggga tttgaccacc ctccgctgga
     3301 tacgctgatt cttgccatgc ctgtgtcatg gaaagggacg ttacagcagt atgcgggacg
     3361 tctacacaga gagcataccg gtaaaagcga tgtcaggatc attgattttg tggataccgc
     3421 gtatccagtg ctgctcagaa tgtgggataa acgccagcgg ggttataagg cgatggggta
     3481 caggattgtg gctgatggtg aagggctttc attctaagga gtactttacc atttttaaat
     3541 aatttgagct ccccgggttg tacgggcaac aaagagcccc agtatacagg cgtcaggaat
     3601 atgttttagt atcaggcttt tcatcgcttt aaaatgacta ccggcagtga gaacgtcatc
     3661 aaaaataaca atccagggcc tgcacccttc cagtaattgt tcatttagtt ggtagtttgc
     3721 cataattgct tctggtgagg gccgatttgc tgctgtatga gaggcaattg ttgatacgtt
     3781 ttgcacaacc agttcccggt aatccagcca gggttcgtag gtttgcgcaa ttcgcagaat
     3841 ttctgtcata cggtcatcgt aagcggcatc tgtacggcat ttggatggtg gtacgggaac
     3901 aaatgtgtat tcctcaagat taccgatatt tgatatgagc atctgagcaa catactcaat
     3961 agcagccttt ttatagtggt aatcagggaa tgcccgacgt tcatacccct ttttaagatt
     4021 gatgattaac tggttagttt tgctgtaagc tgcaccttgt cgggctgtgt attcgccaaa
     4081 gcaataacac agatcaccat cctccagata atgatgttgt ttacgtatca gttcatcaat
     4141 cagagtcaga cgaagtgtca acggagatgc ctcctgatgt cttcgtaatt cctgacccga
     4201 atggcccctt gcgcttcaaa acgggcaggc catgtaattg atgggttacg aaagcaggat
     4261 tccagaataa acaattttcg gccctgggca agtgccgcac gtgcctgagt cagagtacct
     4321 gatgtttctg atgcttcaat aataatcgtt gcttctgtca gtgcagacat cgtgacattt
     4381 cgctccggaa aaaatattcg gttgctccga tagtcctgct ccaggtatcg atgaaacgga
     4441 acctgactga taagcaggtg gttgtcgcga atgtttttct gcagattact gttttgtctg
     4501 ggataatgat gtgaaagtgg tgtcccgata actgcaatcg ttttaccacc cagagccagt
     4561 gccgtctgat gcgcgttggt atccacgcct tcagccagtc ctgaaacgat ggtaaaacca
     4621 tcctcaacca gaaatttgac gactttgcgg gttcgttttg ctccttcttc agagactcta
     4681 cgggaaccaa caacagctac cgagcgagtg ttaatcaggt cccaccaccc ctgatagtaa
     4741 agaagttcta tggggttccg cgcatcccgc agtttttctg gatattctcc ggccccatgg
     4801 acacgtatcc ccagatcgtc aatatcgtaa cgcgcgataa tttctttcag ccggttttta
     4861 aaagcagcaa taatgctgtc tgagacaaag tcagaaggaa gcgagccggt tggtgctgca
     4921 cgaaatttat ccgcaatagt tttgaatgtc gcgccttttt ctgaccataa cgcctcataa
     4981 gccgccattt cctcaaaagg agaaatggct cgggcaaagg gatccagact ggtaaaatcg
     5041 aactgcgaca taagcattca gggataataa acaggatgaa caggtagttt acctgttcag
     5101 gctccggtgt aaaccccgga ggtggatgct gctgataatg ccacaatcga cggaaactgt
     5161 gaaattgtcg taatttttat tcagacttaa ttttattgcg atgccaggtc agggatggtg
     5221 ggttacatca aagatgagaa acgtgcaaaa tgacacctga atcgatcaca gttcggcata
     5281 cgcaaaacgc gttatcagta gtgactggaa tcatcgttat tgcgcatttt ccatttataa
     5341 gaacacactg aggagtttca ggttctcagg agagttatga ctgttttttt actcaaatcc
     5401 acaacacctg aactggaact tgtgttgcat ccctgagtgt tactctgcag gaaacatctt
     5461 ttttaccatc aaaggatgac tgttttcctt tctcccctcc gtaaaacaca actctgatca
     5521 catttctgac atttttccgg attttacata acaggattgt ttctgtatgt tttttatctg
     5581 gtgtaaattt cagcactgac attccactta cgttaattta cactgaatac ccacgacacg
     5641 aggagaatat gcagcaccgg caggataact tactggcgaa cagaacgtcg tttcctggta
     5701 tggtttccgg tcagtacgca ttcaggatcc gtaccttatc tcaggtggta cgctattttt
     5761 ccctcctccc ctgcctttgc attctttcat tttcgtctcc ggcagccatg ctgtctccgg
     5821 gtgaccgcag tg
//
LOCUS       DAZFFG010000201         5786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFFG010000201 DAZFFG010000000
VERSION     DAZFFG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.202"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            110..1660
                     /locus_tag="ACHJ7J_004756"
     CDS             110..1660
                     /locus_tag="ACHJ7J_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFO5002866.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKSSLIWCAEDVRNPGELQNACS
                     YIIDPDSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SDGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSQLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKQLVNSFVALLNKYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQTIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            1686..2138
                     /locus_tag="ACHJ7J_004757"
     CDS             1686..2138
                     /locus_tag="ACHJ7J_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO5002867.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            2291..3565
                     /locus_tag="ACHJ7J_004758"
     CDS             2291..3565
                     /locus_tag="ACHJ7J_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5002868.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGASVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3558..5654
                     /gene="mchF"
                     /locus_tag="ACHJ7J_004759"
     CDS             3558..5654
                     /gene="mchF"
                     /locus_tag="ACHJ7J_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFO5002869.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQIRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TKMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKILVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEESDEVWMTECARASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIISI"
BASE COUNT         1652 a         1070 c         1426 g         1638 t
ORIGIN      
        1 tatgtgtaat taatagagtt ttactgtaat aaggaagaag ctgtcaggac acttacttgc
       61 tcttttatga acactctttt atgaaaatta aaacggagaa tactgccaga tgagtcatca
      121 gtgttcactt tctgaactga atgaaaacct ggtgcctttc acagccaggc agatcaagtc
      181 ctcattaatc tggtgtgcag aggatgtcag aaatccaggc gagctgcaaa atgcctgcag
      241 ttatattatc gatcctgaca gtacggcttc tgccaaagtg ttccatgcag agcgctatgg
      301 tggcagtggt attcagcgta atggaggtgg tgcacgttgt gggtttgatg gtaactacca
      361 ggttaaagga ataggaagta atccgttggt tggtgaaggt actgacgaac gtcattctga
      421 tggtgcactc ggcgctgttc atgcaatata tgaggctttg tggggagaag tactggctca
      481 aatattacct tatagtgctg tgcgggttcg ggcggtttta cttacagatc tctatactga
      541 aaaggcattt gagcgctccg gtaggaaatc acgaagagcc ctgttggtac gtgagcctgt
      601 tgttcgcccg gcgcattttg aacgggcacc atacttccaa gtaaaaccgg agtattccag
      661 tcagttaatt cacgatgcct gtcgggttag atctgtgatc cacaagctgc caggatatct
      721 acctgtacca ccggaagaaa ttgatgctga agcacgaact gatccccgga tttattgcat
      781 tgaggggtta tgtgaactgg cacgtcgtga ggcctggcaa atggcatttt gtcgaacacg
      841 tttcctgaga ttgacaactt ctccttctaa tattgcaatg gatggcagat taatggattt
      901 taacggactc agttgtctgt ttccgggaga ttccccagct gattttgggt ataaactaag
      961 attagctgaa ctggcaaaag aaccgatggt acttatgcaa gggctgtctg atctctgctt
     1021 gtatatcgga aaatatatgt ttgaccctga cttcactctt gcagcccgtt tgaaggttga
     1081 ggagatattt cagaaaactt ttcatgaagc atgttattac tgttatctag aactgttggg
     1141 tattcctgga gaatttataa cacaaaaaga gatacctgat atattgaaac aactggttaa
     1201 cagttttgtt gcattactca ataaatactg cgagaaatca catgcccaag atattgtcaa
     1261 tcaggatggt tcaccattgc aaaagttggt tgtgacgcta atccatcata ggcataatca
     1321 aaagcaggca ctgaatagta gcatcaagaa tgatgtttat ttcaccgttg cacaacagtg
     1381 tttttcccag actatccact ggctgacgca aggcagtacc agacgtcaga taaatgcttc
     1441 attactcctg aaagaaattg aacatcatac catgaaaagg ctgcaaccaa gggaagagct
     1501 gaggaaagag aatatgtgcg aaaaaattgc catcctgctg gataatcatg gcgatgatcc
     1561 ccttttttta caagaagcaa tttctgatat gaaaaatttt atgcttaagt tttccagaga
     1621 tgcatttgga tatcttgaac cgataagaaa cacagtgtaa taaatatata agggaaatag
     1681 taatcatgtc ttatataagg gaaaccatca gaggaaaaga tgaatggact gtttatgaac
     1741 agataggttt tgcggtcagt tgtatgctct acaatcgtaa ttacagtctg tatccggtgt
     1801 taaccattca atactggact gaatatgcga tacagcataa tcagattaaa ttcctgtttg
     1861 attcacgagg ttttccactg gcgtatatta cctgggcata tcttgaggct gatacggaag
     1921 cgcgcctgct cagggatcca gaattcaggt tgcatccgtc tgaatggaat gaagatggaa
     1981 ggatctggat cctggatttc tgttgtaaac caggctttgg tcgaaaagtt attgactatc
     2041 tcatacagct tcagccatgg ggggaaggag aagtacgatg gttaagcagg cgaaagaaaa
     2101 ttgtgacata catccctgag cggctgcata aaacgtagta cctctgaaga tacagagata
     2161 attgtaaatt acggggtaaa tgcatcgctg atactatttt gacaggactc tgtattttct
     2221 ggctttgatt atagcgtttc ggttaatcag tgaaggtatt acaggaaaag tgtgagtaag
     2281 aggagctatt ttgtttcgtc aggatgcttt agaaaacaga aaaatgaagt ggcagggacg
     2341 ggcaatatta cttcccggaa taccactatg gttaatcatg ctgggaagca ttgtgtttat
     2401 tacggcattt ctgatgttca ttattgttgg tacctatagc cgccgtgtta atgtcagtgg
     2461 tgaggtcaca acttggccaa gagctgtcaa tatatattca ggtgtacagg gatttgttgt
     2521 caggcaattt gttcatgaag ggcagttgat aaaaaaaggg gatcctgttt atctgattga
     2581 catcagtaaa agtacacgta gtggtattgt cactgataat catcggcggg atatagaaaa
     2641 ccagctggtt cgtgtggaca acattatttc ccgtctggaa gaaagtaaaa aaataacgtt
     2701 agataccctg gaaaaacaac gtctgcaata cacagatgcg tttcgtcgct catcagatat
     2761 tatacagcgt gcagaggaag ggataaaaat aatgaaaaac aatatggaga attacagaaa
     2821 ctatcaggca aaagggctga ttaataaaga tcagttaact aaccaggtgg cattatatta
     2881 tcagcaacaa aacaatcttc tcagcctgag cggacagaac gaacagaatg ccctgcagat
     2941 aaccactctg gagagtcaga ttcagactca ggctgcagat tttgataacc gtatctacca
     3001 gatggaactg caacggtacg agttacagaa agaactggtt aacactgatg tggagggcga
     3061 aattattatc cgggcgttga ctgacgggaa agttgactcc ctgagtgtca ctgtcgggca
     3121 aatggtcaat accggagaca gccttctgca ggttattcct gagaacattg aaaactatta
     3181 tcttattctc tgggtcccaa atgatgctgt tccttatatt tcggctggtg acaaagtgaa
     3241 tattcgttat gaagcctttc cggcagaaaa atttgggcag ttctctgcta cggttaaaac
     3301 tatatccagg actcctgcgt caacacagga aatgttgacc tataagggtg caccacagaa
     3361 tacgccgggc gcctctgttc cctggtataa agtcattgcg atgcctgaaa agcagattat
     3421 cagatatgac gaaaaatacc tccctctgga aaatggaatg aaagccgaaa gtacactatt
     3481 tctggaaaaa aggcgtattt accagtggat gctttctcct ttctatgaca tgaaacacag
     3541 tgcaacagga ccgctcaatg actaacggga atttcagaca aattataaat cagcttgata
     3601 tgcgctggcg acgtcgtgtt ccggttattc atcagacgga gaccgctgaa tgtggactgg
     3661 cctgcctggc aatgatatgc ggtcattttg gtaagaatat tgacctgata tctcttcgcc
     3721 ggaagtttaa tctctcggcc cgtggagcaa accttgcagg aatcaatgga atagcggagc
     3781 agctggggat ggtcacccgg gctctttcac tggagctgga tgaacttggt gccctcaaaa
     3841 tgccgtgtat tctccactgg gatttcagtc actttgtcgt gctggtcagc gtaaagcgta
     3901 accgttatgt actgcatgat ccggccagag gcagaagata tctcggtcgg gaggaaatga
     3961 gccggtattt tacgggcatt gcacttgagg tctggcctgg aagtgaattc ctggcggaaa
     4021 cccagcagat ccgcataagt ctccgttcac tgattaacag tatttacggt attaaaagaa
     4081 cactggcgaa aattttctgt ctgtcagttg taattgaagc aatcaatctg gtaatgccgg
     4141 tggggactca gctggttatg gatcatgcga ttccggcggg ggacagaggg ctgctgacgc
     4201 ttatttctgc tggcctgatg ttctttatat tgctcagggc agcggtgagt atgctgcgtg
     4261 catggtcctc actggttatg agcacgctca tcaatataca gtggcagtcg ggtctgttta
     4321 accatcttct cagactgccg ctggcgtttt ttgaacgccg taaattaggt gatatccagt
     4381 cgcgttttgg ctcccttgac actttgaggg ccacctttac cacctgtgtg gttggggcaa
     4441 tcatggacag tattatggtt gtgggtgttt ttgtgatgat gctgttatat ggaggatatc
     4501 ttacctggat agtgctcggt tttaccatgg tttacgttct tattcgtctg gtgacatacg
     4561 gctattaccg gcaaatatcg gaagaaactc ttgtcagggg ggcccgggcc agctcttatt
     4621 ttatggaaag cctgtatggt attgccacgg taaaaatcca aggtatggct gggatccggg
     4681 gaacacactg gcttaacctg aaaatagatg cgatcaattc aggtattaag ttaaccaaga
     4741 tggatttgct cttcgggggg ataaatactt ttgttgccgc ctgtgatcag gtggcgattt
     4801 tatggctggg tgcaagcctt gtgatcgata atcagatgac aatagggatg tttgtggcat
     4861 ttggttcttt tcgtgggcag ttttcggatc gggttgcttc gctgaccagt tttcttcttc
     4921 aactgagaat aatgagtctg cataatgagc gcattgcaga tattgcacta catgaaaagg
     4981 aagaaaagaa accggaaatt gaaatcgttg ctgacatgag cccggtttca ctggaaacca
     5041 ctgatttaag ctaccggtat gacagccagt cagcacaggt attcagtggt ctgaatttgt
     5101 ctgtggctcc gggagaaagt gtggctataa ctggtgcctc cggtgccgga aaaaccacat
     5161 taatgaaagt attatgtgga ctgtttgaac cagatagtgg aaaaatattg gttaatggca
     5221 cggatatacg tcaacttgga ataaataatt atcaccgtat gatagcctgt gttatgcagg
     5281 acgaccggct attttcagga tcaattcgtg aaaatatctg cgggtttgca gaagaatcag
     5341 acgaagtatg gatgacagaa tgtgccagag caagtcatat tcatgatgtg ataatgaaaa
     5401 tgccaatggg gtatgaaacg ttaataggtg aactgggaga gggactttct ggtggtcaaa
     5461 aacagcgtat atttattgca cgagccttat accggaaacc aggaatatta tttatggatg
     5521 aggctacaag ttctcttgat acagaaagcg aacgtttcgt gaatgctgct ataaaaaaga
     5581 tgaatatcac ccgggtgatt attgcacaca gagaaactac gttgagaact gttgacagga
     5641 ttatttctat ttaaaattca ctggtgtaac tttgtaagga gttttgtcga tgggggaggt
     5701 taaaaaggat ataaaaataa cagtgattgc ttttgttatc aattacttgt tcttttatat
     5761 cccggagtca ttatatctca gttatt
//
LOCUS       DAZFFG010000202         5760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFFG010000202 DAZFFG010000000
VERSION     DAZFFG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.203"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            16..1005
                     /gene="ldhA"
                     /locus_tag="ACHJ7J_004760"
     CDS             16..1005
                     /gene="ldhA"
                     /locus_tag="ACHJ7J_004760"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5002870.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHJ7J_004761"
     CDS             1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHJ7J_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO5002871.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1535..1801)
                     /locus_tag="ACHJ7J_004762"
     CDS             complement(1535..1801)
                     /locus_tag="ACHJ7J_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO5002872.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHJ7J_004763"
     CDS             2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHJ7J_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO5002873.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKIRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1444 a         1483 c         1488 g         1345 t
ORIGIN      
        1 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
       61 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
      121 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
      181 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
      241 tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct gaaagtagtc
      301 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
      361 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
      421 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
      481 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
      541 tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
      601 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
      661 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
      721 gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
      781 ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
      841 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
      901 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
      961 agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     1021 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     1081 tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg
     1141 cgctaagcct gctgatggcg ggatgtgtaa gtaacgacaa aattgctgtt acgccagaac
     1201 agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata
     1261 aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc
     1321 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg ctagcaatga
     1381 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     1441 taaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     1501 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     1561 caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac
     1621 catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     1681 tactggacat cgacgctttt aaacctggcg ctacatgcgc cgcagtcgcc tgctgaacag
     1741 gctcactact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     1801 tctcttcttc ctctaatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     1861 gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     1921 ttttgccctc tgtttgatca aaacattcat tactctgatg tgggggacac aaaagcgaaa
     1981 atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc agcaatttcg
     2041 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc
     2101 ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta taacccccag
     2161 ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga acgtttgggg
     2221 agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     2281 cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat
     2341 gatcccgacg ctgtacaaac tggcaggcga actcacaccg tttgtcctgc atgtagcggc
     2401 acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg tcatggcggt
     2461 gcgccagacg ggttgcgcga tgttgtgtgc ggcaaacgtc caggaagcgc aagactttgc
     2521 tctcatttcg cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt tctttgatgg
     2581 tttccgtacg tcccacgaaa tcaataaaat tgtcccgctg gccgatgaca cgattcttga
     2641 tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcgctcaacc cggaacatcc
     2701 ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa
     2761 cccatggtac aacgcggtct atgaccatgt tgaacaggcg atgaatgatt tctctgccgc
     2821 gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg aacgggtgat
     2881 tatcctgatg ggctctgcca ttggcacctg tgaagaagtg gttgatgaat tgctaacccg
     2941 tggcgaaaaa gttggcgtgc tgaaaattcg cctgtaccgt cccttctccg ccaaacactt
     3001 gctgcaagct ctgccgggat ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc
     3061 cggtgcccag gcagaaccgc tctatctgga tgtaatgacc gcactggcag aagcctttaa
     3121 taatggcgag cgcgaaactc tgccccgtgt cattggtggg cgctatggtc tttcatccaa
     3181 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     3241 agcgcgcttt acggttggta tttacgatga tgtgaccaat ctgtcactgc cgttgccgga
     3301 aaacaccctg ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg
     3361 cagcgtttct gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc
     3421 acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt ctcaccttcg
     3481 cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     3541 ccaccaattg cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat
     3601 tttcctgctc aacacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaggaagt
     3661 tcaggccgtg ctaaaccaga aaaaagcgcg cttctatgtg attaacgcag cgaaaatcgc
     3721 ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     3781 gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag
     3841 ttacagtagc aaaggtcagg atctggtgga gcgcaactgg caggctctgg cgctggcgcg
     3901 tgaatccgtg gaagaagttc cgttgcagcc ggttaatccg cacagcgcca atcgaccgcc
     3961 tgtggtttcc gatgccgccc ctgatttcgt gaaaaccgta accgctgcga tgctcgccgg
     4021 gcttggtgat gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac
     4081 cacgcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg
     4141 tacccaatgt aaccactgcg ttgccgcttg cccacactcc gctattcgcg caaaagtggt
     4201 accgcctgaa gcgatggaaa acgctcctgc cagcctgcat tcgctggatg taaaatcgcg
     4261 tgatatgcgc gggcagaaat atgtcttgca agtggcaccg gaagattgca ccggctgtaa
     4321 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     4381 tatgatgtcg cggctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     4441 cctaccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat
     4501 tacaccgctg tttgaatatt ccggtgcttg ttccggttgt ggcgagacgc cgtatattaa
     4561 attgctgact cagctctatg gcgaccggat gttgatcgct aacgccaccg gctgttcttc
     4621 aatttatggc ggtaacctgc cctccacacc gtatactacc gatgccaacg gtcgcgggcc
     4681 ggcatgggca aactctctat ttgaagataa tgccgaattt ggccttggtt tccgcctgac
     4741 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg ataaaatccc
     4801 ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc gtgaacaggt
     4861 tgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tgcgtgacgc
     4921 agatgcactg gtagaaaaat caatctggct gattggtggc gacggctggg cttacgatat
     4981 cgggtttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct
     5041 ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc
     5101 agtaactaaa tttggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     5161 gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcaac tgaaccagac
     5221 ggtgaaagcg attcaggaag cggaagcgta tccgggacca tcgctgatca ttgcttatag
     5281 cccgtgtgaa gagcatggtt acgatctggc actcagccat gaccagatgc gccaactcac
     5341 agctaccggc ttctggccgc tatatcgctt cgatccgcgt cgtgccgatg aaggcaaact
     5401 gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt tacttcacga
     5461 gcaacgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     5521 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     5581 aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa aaaaagcccg
     5641 gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca
     5701 acagaaaata aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa
//
LOCUS       DAZFFG010000203         5720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFFG010000203 DAZFFG010000000
VERSION     DAZFFG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.204"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            367..2004
                     /locus_tag="ACHJ7J_004764"
     CDS             367..2004
                     /locus_tag="ACHJ7J_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002874.1"
                     /translation="MVTRTMQQKYQYDRVLSGELHPFPGIYEAVSEQILLLIFKARQL
                     YQDPEYRPWFEEQHRVLSALRANTELLRIFREQRSAEEPKLTIEESTSPQPTPSALTD
                     ALNVMCHLYNGFDEGNISLKTWGAMQEVQTTSTFISPGPRTWKTPELAKFVGDDLFNI
                     LTECKAWSSPIPAETRQGYCHQDLSVAHSCTSELLAHLNQYHEIDESAILARVIGITV
                     NGFNKKNNTDLPEWLSPFKEEHKIFRDCPEQNTGDVIRRKEQLLKTGRTEFVNILCRL
                     ANWYEIESIYDEYVRLYANEIIDWFNKTMKYFKPTDFIRYTWKFATVTPDKDTETGHT
                     TAWEKDYCQITIQRDELLRLIHTNTLQDAVIYLCHCLQEKYKKSSQNETEFPGIKPPA
                     SESEINITDNFEFHSLLELSKDNEKRLETFQKWYEKNKVLLESHKQKRTEKIDVAVRL
                     VGLKAYDLHEGIPDRPKRKVKDGINEDIKADTSLRLSKTNISDTSLNRYRRTVKEIIN
                     NEIDVFLLEQKKKNKRFPYSEDRDVIRPLWGKCLTGQ"
     gene            complement(2091..2687)
                     /locus_tag="ACHJ7J_004765"
     CDS             complement(2091..2687)
                     /locus_tag="ACHJ7J_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002875.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVSL
                     ATFVYETIATKAGVILLSDNEQYEGGKAFWQHIARRSTNLKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            complement(2998..3073)
                     /locus_tag="ACHJ7J_004766"
     tRNA            complement(2998..3073)
                     /locus_tag="ACHJ7J_004766"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3038..3040),aa:Thr,seq:cgt)
     gene            complement(3188..4441)
                     /gene="proA"
                     /locus_tag="ACHJ7J_004767"
     CDS             complement(3188..4441)
                     /gene="proA"
                     /locus_tag="ACHJ7J_004767"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5002876.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(4453..5556)
                     /gene="proB"
                     /locus_tag="ACHJ7J_004768"
     CDS             complement(4453..5556)
                     /gene="proB"
                     /locus_tag="ACHJ7J_004768"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5002877.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         1624 a         1407 c         1293 g         1396 t
ORIGIN      
        1 acaaggcatg gaggagtcgc aggaaggtga aatatttcca tctaatttgt gataatcatc
       61 acaaaaataa gctacaacaa gcggaggaaa agacaatctg ttgctgaaca gacagaagca
      121 ggctacaaat gatcggaaaa tgaagcaaat acgcttatac agaagacgca gaggtaactg
      181 acggagacaa tcacaatcag gagaaaatta tcgtaaataa tgtataaaat ctcaggatat
      241 aaacaaatgt acacaagcta tccacgtaat cagcggctgg ctcaggatta acgtttcatt
      301 tcagtggaca acatcgattc tgtctgacaa cggaccaata tgtagcaagc agaaacttct
      361 gacatcatgg taactcggac aatgcaacaa aagtatcagt atgatcgtgt tttatccggc
      421 gaactccacc cattccctgg catatacgag gcggtttccg aacaaatcct tttgctcatt
      481 tttaaagcac gtcaactata tcaggatcca gaatatcgcc cctggtttga agaacagcac
      541 agagtactct ctgcattacg tgccaataca gagctcctga gaatctttag agaacaaaga
      601 agcgctgaag aaccaaagct tactattgaa gagtctacct ccccccaacc cactccttct
      661 gctcttacag atgcgcttaa tgtgatgtgc catttataca atggttttga tgaaggaaat
      721 atcagtctga aaacctgggg ggcgatgcaa gaggtacaga caacatcaac ctttatcagc
      781 ccaggaccga gaacctggaa aacacctgaa ctggcaaagt ttgtaggaga tgatttattt
      841 aatattctga ctgaatgtaa ggcctggagt tcaccaattc cagcagaaac ccgacagggg
      901 tattgccatc aggatctgtc tgttgcacac tcctgcacct cagagttact ggcacacctg
      961 aatcaatatc atgaaattga tgaatcagca atacttgcca gagttattgg tataacagtt
     1021 aacggattca acaagaaaaa taatacagac cttccagaat ggctatcccc ctttaaagag
     1081 gaacataaaa tattcagaga ttgtccggag caaaataccg gggatgtaat cagacggaaa
     1141 gaacaactgt tgaaaacagg acgtacagaa ttcgtgaata ttttgtgtcg gctcgccaac
     1201 tggtatgaaa ttgagtcaat atacgatgaa tacgtccgtc tttatgccaa tgaaatcatt
     1261 gactggttca acaaaaccat gaagtatttt aaaccaacag attttatccg atatacatgg
     1321 aaattcgcaa cagttactcc ggataaagat acagaaaccg ggcatacaac tgcatgggaa
     1381 aaggattatt gccagataac catacaacgt gatgaacttc tgaggttgat tcacactaat
     1441 acccttcagg atgctgtaat ttatctttgt cattgtctgc aggagaaata caagaaatca
     1501 tcccagaatg agacagagtt tccaggaata aaacctccgg catcagagag tgaaattaat
     1561 ataacagata attttgaatt tcattctctt ctggagctaa gtaaagataa tgagaagcgg
     1621 ctggaaacat ttcaaaaatg gtatgaaaaa aataaagttt tgttagaaag tcataaacaa
     1681 aaacgaacag agaagattga cgttgccgta cgacttgtag ggctgaaagc ctatgatctg
     1741 catgaaggga ttccggatag gccaaaacga aaagttaaag atggcataaa tgaagacatc
     1801 aaagccgata ccagtttacg cctgtcaaag acaaatataa gtgatacgtc gctaaaccgt
     1861 tatcgccgca ctgttaagga gattatcaat aacgaaatcg acgtattcct gttggaacag
     1921 aagaaaaaaa ataaaagatt tccctacagt gaagatagag atgtgattcg acctttatgg
     1981 ggaaaatgcc tgaccggaca gtaactccct gaggggataa cagcccggtt atcccctgtt
     2041 cgttatgcct cggtataccg gagttccagg gacacgagac aatcggcaaa ttatgcggct
     2101 tttccattaa cagattcggc cacaagaaca acaccgtgtt tgtttcgatc aggatgttca
     2161 ctccagattt ctgattcagg aatactttcc ccatcatagc agatccgatc accatcaaac
     2221 ggataatacc gtgcggaatc tgtatctaag ataaacacct taagattcgt tgaacgacgc
     2281 gctatatgct gccagaaggc tttacctcct tcatattgct cgttatcact taatagtata
     2341 acgcctgctt ttgtggctat tgtttcataa acaaacgtag caaggctaac atcacgtaca
     2401 gcgccatcga caacaatccc ttcaatctga gcaacgtcct gttcttccca gaatttccgg
     2461 acaaacaccc cttcaagctg tgaacgacgc tgaagctcca gagtggcaac aaccttatgg
     2521 cgaatatcac catcaataac agaaggtgca gcttcgatta ccgcagcgaa cggagtcttt
     2581 ggatcccaga tgatctggta aagcacaccc tgaatagtta ctccatccag tatcgaaaaa
     2641 cgacgagcta cagtagcagg ggagtaagac attggaatga cagccatttt atggcctaaa
     2701 atttgaaaac attagtatca atactgacct catcagtctg agcatatatt gataaagttt
     2761 ttatgtttac actcaataaa taccttgcca aacaactaat agcgatgtct acattgccat
     2821 tcgacatgct tacaattttt gtcaacaaga aagtgaattt atatcaacgg taagaagtaa
     2881 acacgaggac accaaaacca aatgtacaca cttttgtgct ttgtgtcccc tgtgtgtacc
     2941 caaatgacaa actgcaagaa aatctaggca cacacttatc taacttattg atttaaatgg
     3001 tgccgataat aggagtcgaa cctacgacct tcgcattacg aatgcgctgc tctaccaact
     3061 gagctatatc ggccctgaaa ggacatgttc acgaacgtga atcacggtag acaaggttaa
     3121 aactaaccgg gcgatgcgtc aatggccttg tgaatcaaat ggctactttt gcatcacccg
     3181 gttttattta cgcacgaatg gtgtaatcac caatgccgat ccacttgtaa gtggtcagcg
     3241 cttccagccc cattgggcca cgcgcgtgga gtttttgtgt gcttaccgcc acttccgcac
     3301 ccagtccaaa ctggccgccg tcggtaaaac gcgtagaggc gttaacgtaa acagcggacg
     3361 aatccacttc gttaacaaaa cgctgggcgt tgcgcatatc gcgggtcagg atcgcatcgg
     3421 agtgttgtgt gccgtgttca cgaatatggg cgatggcatc gtcaagatcg ctgacgattt
     3481 tgacgttcaa atctaatgac agaaactcat cgtcatactc ttcggcttta accgccacca
     3541 ccttcgcggg gcctgcctgc aactgtgtca gcgctgctgc atctgcgtgt aatgtcacgc
     3601 cgctttccgc catttgtttg cttaatgcgg gcaggaagct atcggcgatg tttttattca
     3661 ccagcaacgt ttctaccgta ttacatgtgc tcggacgctg agttttcgcg ttgacgatca
     3721 cttttaatgc ttcagcgatc tctgcacttt catcaacgta aatatggcat acgcctatac
     3781 cacctgtgat caccgggatc gtcgactgtt cgcggcacag tttatgcaaa ccagccccgc
     3841 cgcgcgggat cagcatgtcg atgtatttat ccatacgcag catttcactg accagcgcac
     3901 ggtcaggatt atcaatcgcc tgcacggcac ccgccggtaa gccacaggat ttcagggcgt
     3961 cctgaatcac cgccaccgtt gccgcgttag tgcgacaggt ttctttgcca ccacgcagga
     4021 tcaccgcatt accggttttc aggcacagcg aagcgacatc aaccgtcacg ttcgggcgcg
     4081 cttcataaat cacgccaata acccccagcg gtacgcgacg acgctcaaga cgcaggccgc
     4141 tgtccagtac gccgccatcg attacctgcc ccaccgggtc ggcgaggttg cacacctgac
     4201 gtacatcgtc ggcaatgcct ttcagccgtg cgggcgtcag tgccagacgg tcaagcatcg
     4261 cttcgctaag gccattggct cgcgcgtcag caacatcctg ggcgttagcg ttgaggatga
     4321 tttcgctttg tgcttccagt tcatcggcga ttttttccag cacgcgattt ttttcgcggc
     4381 tggaaagttg cgctaattta tacgaggctt gcttcgcggc aatgcccatt tgttccagca
     4441 tcggccagct ccttaacggg taatcatgtc atcacggtga acggcaaccg ggccgtattc
     4501 atatcccagt attgcatcaa tttcttgcga gtggtgtccg gcaatacggc gtaatgcatc
     4561 gctgttgtaa cgactgacgc cgtgggcgat atcgcgacct tcgaggttac aaatgcggat
     4621 gacttcacca cgcgagaaat tgccagtcac gcttttaatg cctttcggca acagggagct
     4681 gccgcgttca agaatggcgg cggttgcccc ttcatctacc gtgatttcac ccgcaggcgg
     4741 cgcaccgaaa atccagcgtt tacggttttc aagcggagtc gcctgggcat ggaacagcgt
     4801 accgacggaa atgccttcca tcacatcacc aatgacgccc ggcttgctgc ccgcggcaat
     4861 aatggtgtcg atacccgcac ggcaagccac gtcagcggcc tgcaatttgg tactcatgcc
     4921 gccagttccg aggcctgaaa cgctgtcacc ggcaatcgcg cgcagtgcgt catcaatgcc
     4981 gtaaacatct ttaatcagtt ctgcctgcgg attgctgcgc gggtcagcgg tatacaaacc
     5041 tttttgatcg gtcagtaaca acagtttatc ggcacccgcc agaatcgccg ccagtgcaga
     5101 aaggttgtcg ttatcgccga ctttaatctc tgccgtagcg acagcatcgt tctcattgat
     5161 taccggaacg atattgttat cgagcaacgc acgcagggtg tcgcgggcgt tcaggaagcg
     5221 ttcacggtct tccatatcag cacgagtcag cagcatttgc ccgacgtgaa tgccataaat
     5281 cgaaaacagc tgttcccaca gttgaatcag tcgactctgc cctaccgccg ccagcagttg
     5341 tttcgaggcg atagtcgctg gcagttccgg gtaacccagg tgctcacgtc cggcggcgat
     5401 cgcgcccgac gtcacaataa caatccgatg cccggcggca tgtaactgcg cgcactggcg
     5461 aacaagttca acgatatggg cacggttcag gcggcgcgat ccgcctgtta gcacactggt
     5521 gccgagtttt accaccagcg tctggctgtc actcatgatt ctctgccatt caattttagg
     5581 aaaaatgata tcaaacgaac gttttagcag gactgtcgtc ggttgccaac catctgcgag
     5641 caaagcatgg tgttttgttg cgcgggatca gcaagcgtag cggcagttgt ttacgctttt
     5701 attacagatt tactaaatta
//
LOCUS       DAZFFG010000204         5641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFFG010000204 DAZFFG010000000
VERSION     DAZFFG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.205"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            536..1102
                     /locus_tag="ACHJ7J_004769"
     CDS             536..1102
                     /locus_tag="ACHJ7J_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5002878.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENMVGFFWVIEWTVDHGFHAHVVFWIDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNEPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            1350..1922
                     /locus_tag="ACHJ7J_004770"
     CDS             1350..1922
                     /locus_tag="ACHJ7J_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002879.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLERALREQSGSRIRRILLR
                     LPWEYDNAFSSRKIWLGMDFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKQYQKQITRNVVLDSRSPDCEERLKELIWAF
                     SDFARDRRGVHDPEARYLAGNPWYPVAGQL"
     gene            1991..2227
                     /locus_tag="ACHJ7J_004771"
     CDS             1991..2227
                     /locus_tag="ACHJ7J_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002880.1"
                     /translation="MLTSITGHDSVLLRVDDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2491..3003
                     /locus_tag="ACHJ7J_004772"
     CDS             2491..3003
                     /locus_tag="ACHJ7J_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002881.1"
                     /translation="MQISEALISEPGKIQRFVQQAVDHWPHLLAVHFTLDSAEGNIDE
                     GQIHAFCTAFYRQVQERITERNHMASPASPLVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASVTVDEQCSQVVDLLQHSWQVISAGGQCRVERCFRVARGDTSGQYVALKTVALSL
                     GLPVVTTISW"
     gene            3092..3715
                     /locus_tag="ACHJ7J_004773"
     CDS             3092..3715
                     /locus_tag="ACHJ7J_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5002882.1"
                     /translation="MRTRIDYLADKYSFTERNESPRLRRQWQDVLEECRLTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGLSQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPFQQIAREVKAPRERLKYALETRLQVTALD
                     GLFWFGSQRIAADILRLRKSGRPVVTTSVEASDNLTGTTRKIPAYHL"
     gene            3811..3999
                     /locus_tag="ACHJ7J_004774"
     CDS             3811..3999
                     /locus_tag="ACHJ7J_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002883.1"
                     /translation="MARSDYDFINLSLEHELNEWLAEVVTRKLRDSFYVDVSWDALNV
                     AYSEHPEWFSELASGDED"
     gene            4363..>5641
                     /locus_tag="ACHJ7J_004775"
     CDS             4363..>5641
                     /locus_tag="ACHJ7J_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO5002884.1"
                     /translation="MQHRQDNLLANRNLLPGMVSGQCAFRLRTFSPVARYFSLLPCLC
                     ILSFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTEGP
                     CFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYITSRA
                     FLTEQDLSGGVLHITVMEGRLQQIRAEGADLPARTLKMVFPGMEGEVLNLRDIEQGME
                     QINRLRTEPVQIEISPGDREGWSVVTLTASPEWPVTGSVGFDNSGQKNTGTGQLNGVL
                     SFNNPLGLADNWFVSGGRSSDFSVSHDARHFAAGVSLPYGYTLVDYTYSWSDYLSTID
                     NRGWLWRSTGDMQTHRPGVSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGSSRKL
                     TSFSVGLNHTHKFLGGVGTLNPVFTRGMPWFGAE"
BASE COUNT         1313 a         1343 c         1481 g         1504 t
ORIGIN      
        1 gattatgttt gttcgtttct gactatcttt tatgttaagc aagataaaaa taatccatgg
       61 tatattttat tctggtatcg tgtgccgtta acgctcatgt tttgaagtga ttcgttttta
      121 acaaattaaa tgagagtgta taattttatc aggttataga tatgtgttgg ccttgtacac
      181 ggttttttca accactgaga gcaagtttgt agtttatgat gttattggtc gtaatatgtt
      241 tcttaatctc ctggtagtgg gtttttatcg cgtgttttgc agtatttcgt gatgtttatt
      301 gattctgtat tttattcgct cctcgtttat ctcatctctt tagtcaatac catcagataa
      361 tccatttctt tctgcataat gctgcgtatc gttaataacc cgtcgtatcc attctgctac
      421 agcatgcctg ataaataccg tctgtaagtt attaccgttt tagatctgat tatgagcgaa
      481 agcattaatt cgttcacaga gcttaaaaca tcattaactt tcaggagtca tcaacatgcc
      541 taaatcttac acaccaaact ggttttttac cgctttactt gacaatcaca tcaatcaaat
      601 gatggcacgc tattcctgcc tgcgggcctt acggatggat ttcttctaca ggaaagatac
      661 gcccgatttc ttacaacctg atcatcgctg gcttgaattg cagttgcgta tgctgctgga
      721 gcaggttgaa caatttgaaa atatggttgg cttcttctgg gtgattgaat ggacggttga
      781 tcatggtttt catgcgcatg tggttttctg gatcgatcgt cagagggtta aaaaaatata
      841 tccctttgcg gagcggatta cggaatgctg gcggtctatt acgcataaca gcggttcggc
      901 acaccgctgt acatatcagc cgcattatac atacaacatc aacattcctg tgcgtcataa
      961 cgagcctgaa agcatcgata atattcgcgg tgctctgcat tatctggcga aagaagagca
     1021 aaaagacggg ttgtgtgcct acggctgcaa tgaagttcct gaacgtcctg ctgcagggcg
     1081 tcctcgtaag cctcacttct gaagcttaag gcctgagcct tcgctcctgg aaacactccg
     1141 tcggtaaaaa cttaccgcct tgattaatga tgtgaactga agtcaacgga gatcattcat
     1201 cctgaacctg catccggtgt tttgttcctt gtcttcccgt tctgcttcgg ttcttcactt
     1261 attccatcaa tctcattccg caagccataa cacgtcagct cattcacggg caggacgcat
     1321 tgtgggctgc gcataacgga acatatctta tgaatgctat tccttatttc gactatagcc
     1381 tggcaccctt ctggccatct tatcagaaca aagtcatcgg cgtccttgag cgtgcgctgc
     1441 gtgagcagtc cggctcacgg atacggcgga tcctgcttcg tctgccgtgg gaatatgaca
     1501 acgccttcag cagcagaaag atctggttag gcatggattt tatcgaaacc gtcagtgcgc
     1561 tgatgaatgc aacccccgga cgtgatctct gctggctcct gacccgtcat cctgaaaaac
     1621 ctgaatacca cgtggtgctg tgcgtcagac aggagtattt cgatggccct gaactggacc
     1681 ggttgatact ggatgcctgg agtaacgtac tgggcttcgc gtcaccgggt gaagcaaagc
     1741 agtatcagaa gcagatcacc cggaatgtgg tgctggacag tcgttcaccg gactgcgaag
     1801 agagactcaa agagttgata tgggcgttca gtgatttcgc ccgcgatcgt cgtggtgtgc
     1861 acgatccgga agcccgttac ctcgccggta atccctggta tcccgtagca ggccagctct
     1921 gaaagctcag ttcagcagat tcccccgcat tcgcctaacc ttcataaatc tcacagagga
     1981 tattctgacc atgttgacct caataacagg ccacgacagc gtattgctgc gtgtcgacga
     2041 tcccctgatc gacatgaact acatcaccag tttcaccggt atgacagata aatggtttta
     2101 caagctgatc agtgaaggac attttcctaa acccatcaag ctgggccgca gcagccgctg
     2161 gtacaaaagc gaagtggagc agtggatgca acagcgaatc gaggagtcac gaggagcagc
     2221 agcatgaaac gtgttgtaat gccagtacgc tggcaatgtg cgaaatgccg gcgctggtat
     2281 tgtggaaccc ggccctgccc ctggtgctgg cgatattccc gttcatcttc ctgctggtac
     2341 cttccggtca gcagcgggtt gttatccctt cctgactgat tcgtcattct attcttattg
     2401 attataactg gcattacacc ggtgccggcg tgcttttctg tagatctgca ccggtttaac
     2461 aaaattagac aaggtttgaa ggaatattct gtgcaaataa gcgaggccct aatttcagaa
     2521 ccggggaaaa tccagcgttt tgttcagcag gcggtggacc actggccaca tctgctggca
     2581 gtccacttca cgcttgattc ggctgaaggg aatattgacg aggggcagat tcatgcattc
     2641 tgtactgcct tttaccgaca ggtgcaagaa cgtattactg agcgtaatca catggccagt
     2701 ccggcatcgc cgctggtatt acgctggttg cgggaacaac atggaggagc aacaattcga
     2761 tgcctgttgc tgctcagcca gacgagtatt tgtcacccgc gagccagtgt cacagttgat
     2821 gaacaatgtt cgcaagtggt ggatttactg caacatagct ggcaggtgat aagtgctggc
     2881 ggacaatgcc gggtggaaag gtgttttcgg gttgcccggg gtgatacatc cggtcagtat
     2941 gttgcgttaa aaacagtcgc attgtctctg gggttaccgg ttgtgacaac aatcagttgg
     3001 taatggacgt gtgaaccatc tgcgcttccg ttgattttta tggactgata aagttttgcc
     3061 agctgaatct ttatacggaa tgctcttcag tatgcgtaca cgaattgact atctggctga
     3121 taaatactct tttaccgaac ggaatgaatc tccacgcctt cgccggcagt ggcaggatgt
     3181 tctggaggag tgtcggctga cagaggccgg accagaagaa cggctgcgta ttgccctgct
     3241 gaatgtggat tacgtcacca gttttgaact gccttttcgg ctgcttttaa cccgcacgcc
     3301 gcaactgatt gccgcgcttc gggaagaatg gggcctcagc cagaaaaatg tggtgttcaa
     3361 cgataaacgg tttggctgcg tgtacagcct gaaggccagc ctttccggtg tgccggatac
     3421 ataccggtat catctgtctc atcgtattcg ccgggtggtt gggaatgaaa acacatcatt
     3481 gccatttcag cagattgccc gggaagtgaa agcgccccgt gaacgactga agtatgcgct
     3541 ggagacgaga ttgcaggtga ccgcactgga cggcctgttc tggttcggca gccagcgcat
     3601 tgcggctgat atcctgagac tgagaaagag cggaaggccg gtggtgacaa cgtccgtgga
     3661 agcgagcgat aacctgacgg gaacaacccg caaaataccg gcataccatc tctgacattg
     3721 cgatgaaggg cagatttcac cttgacaggg gcagagtgcc gctttttata ctttattccc
     3781 gtgtctgaaa aatatgcaaa ggaaacggga atggcaaggt ccgattacga ttttatcaat
     3841 ctgtctctgg aacatgaatt gaatgagtgg ctggcagagg tggttacccg caaattgcgg
     3901 gacagttttt atgtggacgt ctcctgggat gcgctgaatg tggcatacag tgaacaccct
     3961 gagtggtttt cagagcttgc ctccggggat gaggattaac aggcaaatta tgctgctatc
     4021 gggcagagtg attacctgca gggatttcca ttcataagaa tacgccgctt cgggaaagct
     4081 ccggttctcc ggagagttac gattattttt actcaaattc acaacacctg cactggaact
     4141 tgcgttgtgt cccggattgt tactccgcag gaagcatcct ttttaccata cggatgtttg
     4201 ttttccattt cccctccgga aaatacaact ccgatcacat ttctgatatt ttccccggat
     4261 tttacataac aggattgttt ctgtatgttt tttatctggt gtaaatttca gcactgacat
     4321 tccgcttacg ttaatttaca ctggataccc cacgaggaga atatgcagca ccggcaggat
     4381 aacttactgg cgaacagaaa tttgttgcct ggtatggttt ccggtcagtg cgcattcagg
     4441 ctccgcactt tctctccggt ggcacgctat ttttccctcc tcccctgcct ttgtattctt
     4501 tcgttttcgt ctccggcagc catgctgtct ccgggtgacc gcagtgcaat tcagcagcaa
     4561 cagcagcagt tgctggatga aaaccagcgt cagcgtgatg cgctggagcg cagtgcgccg
     4621 ctgaccatca cgccgtctcc ggaaacgtct gccggtactg aaggtccctg ctttacggtg
     4681 tcacgcattg ttgtcagtgg ggccacccga ctgacgtctg cagaaaccga cagactggtg
     4741 gcaccgtggg tgaatcagtg tctgaatatc acggggctga ccgcggtcac ggatgccgtg
     4801 acggacggct atatacgccg gggatatatc accagccggg cctttctgac agagcaggac
     4861 ctttcagggg gcgtactgca cataacggtc atggaaggca ggctgcagca aatccgggcg
     4921 gaaggcgctg accttcctgc ccgcaccctg aagatggttt tcccgggaat ggagggggag
     4981 gttctgaacc tgcgggatat tgagcagggg atggagcaga ttaaccgtct gcgtacggag
     5041 ccggtacaga ttgaaatatc gcccggtgac cgggagggat ggtcggtggt gacactgacg
     5101 gcatcgccgg aatggcctgt cacagggagc gtgggcttcg acaacagcgg gcagaagaat
     5161 accggaacgg ggcagttaaa tggcgtcctt tcctttaata atccgctggg gctggctgac
     5221 aactggtttg tcagcggggg acggagcagt gacttttcgg tgtcacatga tgcgaggcat
     5281 tttgccgctg gtgtcagtct gccgtatggc tataccctgg tggattacac gtattcatgg
     5341 agtgactatc tcagcaccat tgataaccgg ggctggctgt ggcgttccac gggagacatg
     5401 cagactcacc gaccgggagt gtcgcatgtc ctgttccgta acggggacat gaagacagca
     5461 ctgaccggag gtctgcagca ccgcattatt cacaattatc tggatgatgt tctgcttcag
     5521 ggcagcagcc gtaaactcac ttcattttct gtcgggctga atcacacaca caagtttctg
     5581 gggggtgtcg gaacactgaa tccggtattc acacggggga tgccctggtt cggcgcagaa
     5641 a
//
LOCUS       DAZFFG010000205         5400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFFG010000205 DAZFFG010000000
VERSION     DAZFFG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.206"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            140..1198
                     /gene="citC"
                     /locus_tag="ACHJ7J_004776"
     CDS             140..1198
                     /gene="citC"
                     /locus_tag="ACHJ7J_004776"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO5002885.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTKG
                     HRYLIQQDAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1213..1509
                     /gene="citD"
                     /locus_tag="ACHJ7J_004777"
     CDS             1213..1509
                     /gene="citD"
                     /locus_tag="ACHJ7J_004777"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO5002886.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1506..2414
                     /gene="citE"
                     /locus_tag="ACHJ7J_004778"
     CDS             1506..2414
                     /gene="citE"
                     /locus_tag="ACHJ7J_004778"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO5002887.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2425..3957
                     /gene="citF"
                     /locus_tag="ACHJ7J_004779"
     CDS             2425..3957
                     /gene="citF"
                     /locus_tag="ACHJ7J_004779"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO5002888.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3961..4512
                     /gene="citX"
                     /locus_tag="ACHJ7J_004780"
     CDS             3961..4512
                     /gene="citX"
                     /locus_tag="ACHJ7J_004780"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO5002889.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNQGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4487..5365
                     /gene="citG"
                     /locus_tag="ACHJ7J_004781"
     CDS             4487..5365
                     /gene="citG"
                     /locus_tag="ACHJ7J_004781"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO5002890.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
BASE COUNT         1244 a         1529 c         1428 g         1199 t
ORIGIN      
        1 tgtatagcga aaggagaaaa agatatacct cgatcacccc cctttctccc aagtgaaaat
       61 aaaaggttat cagtttgcaa cattgaacaa cattcgttgc aaatcgataa caacatgcac
      121 cttcaggata ctatttatta tgttcggcaa tgatattttc acccgcgtaa aacgttcaga
      181 aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac
      241 cacagtcgaa gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat
      301 tgccggaaat attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc
      361 gctgacatta gccactgaac tgataaacct cgcctatgag cggcacagca cgcatctgtt
      421 tatttatacc aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgttgac
      481 cagcgtacca ggcgtgatgg tgctgatgga aaacagcgcc acgcgattga aacgctatgc
      541 cgaatcgctg aaaaaatttc gtcatcccgg gaacaagatt ggctgcattg tgatgaacgc
      601 caatcccttt acgaagggtc accgttatct gattcaacaa gatgcggcac agtgcgactg
      661 gttgcatctg tttttagtca aagaagattc ttcacgcttc ccctatgaag accggctgga
      721 tctggtgtta aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata
      781 catcatctcc cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca
      841 ttgttacacc gaaattgatt tgaagatttt ccgtcagtac ctcgctcccg cgctgggtgt
      901 aactcaccgc tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga
      961 tatgcgctac tggctggaaa cgccgactat ctccgcaccg cccatcgaac tggttgaaat
     1021 tgagcggctg cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc
     1081 gaaaaacgat ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca
     1141 gaacctgctt gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga
     1201 aacaggtgaa aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat
     1261 gtgatgatac gcatcgcccc actcgatacg caggatatcg acctgcaaat caatagcagc
     1321 gttgagaaac agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac
     1381 aacgtgcgcg gcgtacagct gaatgtcgat gacaaaggcg cactggactg cattttacgt
     1441 gcacgactgg aagccctgct ggcacgcgcc agcggtatcc cggctctgcc atgggaggat
     1501 tgccaatgat ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt
     1561 ttgtgcctgg tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc
     1621 tgatgtttga cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg
     1681 tttatcacgc gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtcaacg
     1741 cgctggattc cgaatggggt gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg
     1801 ttgtgcgtct gccgaaaacc gataccgctc aggatgttct ggatatcgaa aaagagatcc
     1861 tgcgtatcga aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg
     1921 aatctccgct gggcattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg
     1981 gtatcgccct cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag
     2041 gaactgaact gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc
     2101 aggcgttcga taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg
     2161 cccacatcaa acagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc
     2221 tgctgcacaa cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag
     2281 aagccgctga agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg
     2341 tggacggccc ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca
     2401 tccgcgaaga ataaggcaat caaaatgacg cagaaaattg aacaatctca acgacaagaa
     2461 cgggtagcgg cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca aaactcgcca
     2521 aagcaaacct accaggctga aaaagcgcgc gatcgcaaac tgtgcgccaa cctggaagaa
     2581 gcgattcgtc gctcaggttt acaggacggc atgacggttt ccttccatca cgctttccgt
     2641 ggcggtgacc tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac
     2701 ctgaccctgg cgtccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc
     2761 cagggcgtgg ttacccgcat ttatacctcc ggcctgcgtg gtccactggc ggaagagatc
     2821 tcccgtggtc tgctggcaga accggtgcag atccactctc acggcggtcg tgtgcatctg
     2881 gtacagagcg gcgaactgaa tatcgacgtg gctttcctcg gcgtcccgtc ctgtgatgaa
     2941 ttcggtaatg ccaacggcta caccggtaaa gcctgctgcg gctccctcgg ctatgcaatg
     3001 gttgatgccg acaacgcaaa acaggtcgtg atgcttaccg aagaactgct gccttatccg
     3061 cataatccgg caagcattga acaagatcag gttgatttga tcgtcaaagt tgaccgcgtt
     3121 ggcgatgctg caaaaatcgg cgctggcgcg acccgtatga ccactaaccc gcgcgaactg
     3181 cttattgccc gtagcgctgc ggatgtgatt gtcaactctg gctacttcaa agaaggtttc
     3241 tccatgcaaa ccggtaccgg tggcgcatcg ctggcggtaa cccgtttcct ggaagacaaa
     3301 atgcgtagcc gcgatattcg cgccgacttc gccctcggcg gtattactgc aacgatggtt
     3361 gacctgcacg aaaaaggtct gatccgcaaa ctgctggatg tgcagagctt tgacagccat
     3421 gctgcgcaat cgctggcccg taaccccaat cacatcgaaa tcagcgccaa ccagtacgct
     3481 aactggggtt cgaaaggcgc atcggttgat cgtctcgacg tggtggtact gagcgcgctg
     3541 gaaattgaca cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt actgcgcggc
     3601 gcttccggtg gtcactgcga taccgcaatt gcctctgcgc tttccatcat cgtcgcaccg
     3661 ctggtacgcg gccgtattcc gactctggtg gataacgtgc tgacctgcat caccccgggc
     3721 tccagtgtcg atattctggt cacagaccac ggtatcgcag ttaacccggc acgtccggaa
     3781 ctagcagaac gtctgcagga agcgggcatt aaagtggttt ccattgagtg gctgcgcgaa
     3841 cgtgcgcgtc tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc
     3901 gttgtgcgtt accgcgatgg ctcggtgatc gatgttgtgc atcaggtgaa ggaataagcc
     3961 atgcacctgc ttcctgaact cgccagccac catgcggtgt caattcccga gctgctcgtc
     4021 agccgggatg aaaggcaagc acggcaacac gcctggctca agcgccatcc tgttccactg
     4081 gtctccttta ccgtggttgc gcctgggccg attaaagaca gcgaggtcac acgccgaatt
     4141 tttaatcaag gcgtgacagc cttgcgtgcc ttagccacaa agcagggctg gcaaattcag
     4201 gagcaggctg cactggtttc cgccagcggg ccggagggca tgttgagcat tgccgccccg
     4261 gctcgcgacc tcaagctcgc caccattgag cttgaacata gtcatcctct cgggcggtta
     4321 tgggatatcg atgtcctgac gcccgaaggc gaaattctct cccgccgcga ctattcactg
     4381 ccgcctcgcc gctgcctgtt gtgcgagcaa agcgcggccg tctgcgcgcg tggaaaaacc
     4441 catcaactga ccgatttact caaccgcatg gaggcactgc tgaacgatgt cgatgcctgc
     4501 aacgtcaact aaaaccacaa agcttgcgac gtcattaatc gatgagtacg ccctgctggg
     4561 ctggcgcgcc atgctgactg aagtcaatct gtcaccgaaa ccaggcctcg tggatcggat
     4621 taactgcggt gcgcacaaag atatggcgct ggaagatttc caccgcagcg cgctggcgat
     4681 tcagggctgg ttaccccgtt tcattgaatt tggtgcctgt agtgcagaaa tggcaccaga
     4741 agcggtactc cacgggttac gcccaattgg tatggcttgc gaaggtgata tgttccgcgc
     4801 tactgcgggc gtaaacacgc ataaaggcag cattttttct ttagggctgc tatgtgcggc
     4861 aattggccgt ttgcttcagc tcaaccagcc ggtaacgcca acaaccgttt gttctacggc
     4921 ggcaagtttc tgccgtggcc tgaccgatcg cgaactgcgt accaataatt cacaactgac
     4981 ggcaggtcaa cggttgtacc agcagcttgg ccttaccggc gcacgcggtg aagccgaagc
     5041 gggttatcca ctggtgatca accacgcctt gccgcattac ctcactctgc tggatcaggg
     5101 gttagatcct gaactggcat tgctcgatac cttgctccta ctgatggcga tcaacggcga
     5161 taccaacgtt gcatcgcgcg gtggcgaggg gggcctgcgc tggctacagc gcgaggcgca
     5221 aacattattg caaaaagggg gcattcgaac ccccgccgat ctcgattatc tccggcagtt
     5281 cgacagggag tgtatcgaac gaaatctcag tccaggcggc agtgccgacc tgctgatcct
     5341 tacctggttt ttagcacaga tttaattatt taagcacttg ataaattttg aaatattaat
//
LOCUS       DAZFFG010000206         5293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFFG010000206 DAZFFG010000000
VERSION     DAZFFG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.207"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            553..933
                     /locus_tag="ACHJ7J_004782"
     CDS             553..933
                     /locus_tag="ACHJ7J_004782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002891.1"
                     /translation="MMQKGLLYKNEEISFVDGYELIPERRFPVLLEATTLSEMELADY
                     CQIMSSFVEQVKELRAISIQAHEMKESRCADKELRKARIRLRKLEKELLRKDKAQAEA
                     AVLLMLREKFNVLWNTVGKSDISS"
     gene            complement(948..1874)
                     /gene="ltrA"
                     /locus_tag="ACHJ7J_004783"
     CDS             complement(948..1874)
                     /gene="ltrA"
                     /locus_tag="ACHJ7J_004783"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO5002892.1"
                     /translation="MGSEGETRAVSSRDEALSASHDPEGLGRANLLQQVLSRENMVEA
                     WKRVKANKGSAGADGLTIEQTVEHLKTRWPYIREALLNGTYRPQPVRRVEIPKPTGGT
                     RELGIPTVTDMLIQQALLQVLQPLIDPTFSEFSYGFRPGRSAHDAVLQAQRYAQDGFR
                     VVVDVDLEKFFDRVNHDILMDRLARRIADKDVLRLIRRYLQAGIMADGVVMARDEETP
                     QDGPLSPLLANVLLDEVDRALQQRGHCFVRYADDCNVYVRSQKAGEHIDAPTLNCVDY
                     TVLHPMLKCYDGNTSDTGGLFFLTPSQHCWSY"
     gene            complement(2342..3544)
                     /locus_tag="ACHJ7J_004784"
                     /pseudo
     CDS             complement(2342..3544)
                     /locus_tag="ACHJ7J_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            3552..3977
                     /gene="tnpA"
                     /locus_tag="ACHJ7J_004785"
                     /pseudo
     CDS             3552..3977
                     /gene="tnpA"
                     /locus_tag="ACHJ7J_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            4058..4312
                     /locus_tag="ACHJ7J_004786"
                     /pseudo
     CDS             4058..4312
                     /locus_tag="ACHJ7J_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(4341..4972)
                     /locus_tag="ACHJ7J_004787"
                     /pseudo
     CDS             complement(4341..4972)
                     /locus_tag="ACHJ7J_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(5011..5193)
                     /locus_tag="ACHJ7J_004788"
                     /pseudo
     CDS             complement(5011..5193)
                     /locus_tag="ACHJ7J_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1301 a         1337 c         1224 g         1431 t
ORIGIN      
        1 gacaaaaagt accttactac atttactaat ataatgcatg ttacatgatt ttattgtgta
       61 aatgtttatt tctgacgtac cctaacatct gtcacacttc tggaagttaa tgttattttt
      121 tctgtatatt aacttgaatt tctacctgaa acgatgacag aataaaatag gaattataat
      181 attttatatt tatgaacatt tccgacactt ttcgtctgat ttttatgcat tcagagttgt
      241 tgcttatcaa tataataatg ctttgcaaca agtatacagg aatattatct catggatatt
      301 atacaaggaa cattattatc aattgcgagc tgtacgacat acgcattatt gtgaaggaaa
      361 acagtgttct actgtgtctg tatagttgtc accttaattt ttacatagga gatagattat
      421 gcctcacatg cgatacaatt aatataaagg acaaaagtta catggctatt accacctgat
      481 aggtgctcta tacggcatat ttccagtaaa ctgaactaaa caccttcagt aatagcaaga
      541 tggagacttg acatgatgca gaaaggcctg ttatataaaa atgaggaaat atcctttgtc
      601 gatggatatg agttgatacc ggagcgaaga tttccggttt tacttgaagc cacaacatta
      661 tctgaaatgg aacttgctga ttattgtcag ataatgagct catttgtaga gcaagttaaa
      721 gagttgcgag ctatatccat tcaggctcat gaaatgaaag aaagtcgctg tgctgataaa
      781 gaattacgca aagcaagaat ccggttacgt aaactggaaa aagaacttct ccgtaaagat
      841 aaagcccagg cggaagcagc tgttcttttg atgttaaggg aaaagttcaa tgtcctctgg
      901 aacactgtgg ggaaatctga catctcttct taagtagcag aagattatta atatgaccag
      961 caatgttgcg aaggagtaag aaaaaacaag cctccagtat cactggtatt tccatcataa
     1021 cacttaagca ttggatgcag aacagtgtaa tccacacagt tcagagtcgg ggcatcaata
     1081 tgttcgcccg ccttctgact gcgaacgtac acgttgcagt cgtcggcgta ccgaacgaag
     1141 caatgaccgc gctgttgcag cgcccgatct acttcgtcca acaacacatt ggccaacagc
     1201 ggagacagtg gaccgtcttg cggcgtctcc tcgtcacgcg ccattaccac accatccgcc
     1261 atgatcccag cttgcaggta gcgccgaatc agccgtagca cgtctttgtc ggcgattcgc
     1321 ctggcaagtc ggtccatcag aatgtcgtgg ttgacccgat caaagaattt ctcaaggtca
     1381 acatcaacta ccacccggaa tccatcctgc gcgtaccgct gtgcttgtag cactgcgtcg
     1441 tgcgcgctcc ggcccggccg gaagccatag ctgaactccg agaatgtcgg gtcgatcaac
     1501 ggttgcagga cttgtagcaa cgcttgctga atgagcatgt cagtaactgt tggaatgccc
     1561 aattcccggg tgccaccagt cggtttcgga atctccacgc gacgcacagg ctgaggccga
     1621 tatgtcccgt tgagaagcgc ttcccgaatg tatggccagc gagtcttgag gtgttcaaca
     1681 gtctgctcga tggtcaatcc atcagcacct gcacttccct tgttggcctt gacgcgtttc
     1741 cacgcctcga ccatgttctc gcgcgagagc acctgttgaa gcaggttcgc tcgccccaag
     1801 ccttccgggt catggctcgc cgacagtgct tcgtcacgcg acgagaccgc gcgggtttca
     1861 ccctcgctgc ccacgatgcg cctgggcttc cccggcttct gacgcaatgt cctgtcaaga
     1921 gtcatatctt ctggcttttc ttctcgttcg gcccttcgtc ggagtcctga ctactacggc
     1981 ctctgctgac ttctcgctcc gtcttacgac gttgtccttt caggcatgag gcgagatctc
     2041 cccaggtaag aacgcgatcc ttccccgcac aactgccgga tctacgcgac ttcgactcga
     2101 ccacgaaagc ttcgcagtga tgtgcctgct cgccttgctt catcgcgcct catatccgat
     2161 tcttgttcat cggctcacgg gtttattccg tgcttcctcc ccacgcccgg tcgccctcac
     2221 gcagttgcac ttcctttcgc tcgctgtggt cagctcgcgg gaggactttc acctccaaaa
     2281 tcgcgcccat gctgggcgca cacatcctcc acgccctgaa ggacgtgggt ccctgctacg
     2341 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctg caacgagcgg cctgactgca
     2401 ccatctctca acaagcaaga atggcgtgcc ccgccgctaa aatgttacga gcgccattta
     2461 catcggcgtt cgctgtatat ccacatacct ggcatctgaa ttggctttgt gacaggcgat
     2521 tttctttcgc tgtatgacca cagcacgcgc aacgctggcg tatgtacgct ggcggaacag
     2581 caagcacctg accgccacgc cagagctgct tgtactcaag ctggcggcgc atttcatacc
     2641 agccctgatc cagtatcgaa cggtttaaac ctgattttgc ccggacattg tgccccggct
     2701 ggctgaccgt acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta
     2761 tcattgcgtg gtttttgctg acggtcgttg tgactttgtg aaggtagtcc ctgcggatat
     2821 ttgcgataca ggaattcagt cgctgtattt tgcgtttctg cttctgccag ttgttgctga
     2881 atttgacctt gcggcttaac tgtcgctgaa gtctcgccag cgtcttctgg tttttctgca
     2941 aactgtttac aggctcaaag actgtgccat ctgacagcgt ggcgagttta gccacgccag
     3001 catccagccc gaccattgat gctgaagggt gaaccggagt ggatacttca ctttctgtct
     3061 gaatactgat gtaccactta ccgcaggact ggctgacagt gacatttttc acaacacccg
     3121 tgacctgacg gctattccgg tagcacatcc agccaggttt cggcagacgg ctgttttcct
     3181 gatcgagctt aacgccctgc gggtagcgga atgcatcatt ctgtccccgc tttttgaatc
     3241 ggggaaaagc agcccgattc cggaagaagt ttttgtaagc ccgctcaagg tatttcagtg
     3301 actgttgtaa tggctgtgag ggcgaatctt taagccattg cgtttcagtg gcgtttttcc
     3361 actcaaccag ccaggaagcc attttcccgt aagggatgta tttattacca gcctcatgat
     3421 tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc
     3481 tcatttcaca ctcctgttga ccaccgggtc ttaactggaa tttaaattcc tgtaatcgtt
     3541 tcacaggtca aatgacactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc
     3601 attgtgtttt cctgatgcat gttcacctgg tctttgtcac cagataccga cgtcagattt
     3661 ttgactatga cgcgacagaa aaactacgca cttacttttc aaatgtatgt gctgattttg
     3721 aagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta attaactatc
     3781 ctcccgaact ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg ggtaggttac
     3841 tgtgacgaga tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtc
     3901 ctggctattt tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc caatacattg
     3961 aacaacagca aacacctggt caggtggaaa accgcgcctt atatccccgc cctgaaggac
     4021 agggttttac gccgcaccgg ataaagcgga gtcggggggc gtccatctgg tcaaactgga
     4081 tcagtggttc gccggctcaa aaacctgcca ttgttgcggt cacaggatgc cagaaatacc
     4141 actcaaagta cgcgaatgga aatccccctg ctgtaatgcc gaacatgacc gcaatattaa
     4201 cgcggcgatt aacatacagc acaagggtat aacagaatta atggcggcgg gtcacgtcgt
     4261 caaagcctat cgaggcttac gtaaatctgg ttcagtgcca gttgcagcct gagaagtggg
     4321 aagcctcgcc gtttacggtg ggtagcagtc actggcccaa cgtccggtat ttccatcagt
     4381 ctctgacagg tttcattctg tcgggctaca cagcgaagct gtttttcaaa gcgttcagct
     4441 cactcaagct gttatattga tcttccagta cggtgataag atattctgac gacctcgctt
     4501 tcatccgctc cagcgccacc gggaactccc ggaccatctc tgcctggcct ttgtggatgg
     4561 tttaaccgaa ctccagcaac gtaccgtgca gggcattaat ttgcgcagtc cagaacttca
     4621 ccagtggtct gcacgatcgg tgtagaacca gtattgactg ctgttcttct gtcttaacgg
     4681 agatttcctt accgggttgc tgtaccgcta tccagatagc ccgcgcatcc atcatgtcat
     4741 ttttgttact catgacaaac gttttacaaa atgggccggt aacaaccgga tcttgtgccc
     4801 ctgctttgtt aattcacgag gtccagtgct gagaggcatc acaggcctcc ataccaatca
     4861 gacagggctc cagattaatg aagaacgtca ggaaatcctg acgacgaacc tgcttatcca
     4921 ctacttaacc tgtgttctca tcaatgaagc tgtatttccc ttcttctatc acagtgtccc
     4981 attactttat ggggtgggat gtgttcatta catttctttg aacgcagcag ccatctcata
     5041 ggtgcacata caacgagagg atctggttac ctatcccggt aaaacagggc tgatttttct
     5101 ttaccagtta tggttcaaag gaccgtcacg atcgagcgga gtacgcacgt cacctgcgat
     5161 aacggtcttt gtggaataac cattgtggga gttaattaca tttaatatta aaaagaatac
     5221 catagagata tagttgcatt ataaaactaa tatttgtagt gataatattt gtagtgattt
     5281 tatttaaaaa tta
//
LOCUS       DAZFFG010000207         5288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFFG010000207 DAZFFG010000000
VERSION     DAZFFG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.208"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(86..2590)
                     /gene="copA"
                     /locus_tag="ACHJ7J_004789"
     CDS             complement(86..2590)
                     /gene="copA"
                     /locus_tag="ACHJ7J_004789"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO5002893.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITKAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            2852..3784
                     /gene="glsA"
                     /locus_tag="ACHJ7J_004790"
     CDS             2852..3784
                     /gene="glsA"
                     /locus_tag="ACHJ7J_004790"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO5002894.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGESDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            3787..5079
                     /locus_tag="ACHJ7J_004791"
     CDS             3787..5079
                     /locus_tag="ACHJ7J_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5002895.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         1129 a         1474 c         1402 g         1283 t
ORIGIN      
        1 cggataaggc gttcacgccg catccggcag tcatgcgtcg atgccaaatg cgccgcccta
       61 aagcagcgca tccgcaatga cgtacttatt ccttcggttt aaaccgcagc aaccggttgg
      121 cgttactcac tacggtaatc gacgagagcg ccattgccgc tccggcaact accgggttaa
      181 gcagtgttcc agtaaacggc cacaaaatac cggcggcgac cggaataccg atgctgttgt
      241 agataaacgc gccgagcagg ttctgtttca tgttgcgcag cgttgcgcgg gaaatagcga
      301 gcgcatccgc aacacccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg
      361 caacatcact gccgccaccc atcgcaatgc cgacatccgc ctgagccagc gctggcgcgt
      421 cgttaatgcc gtcgcccacc attgccacct gacgtccttc actttgcagg cgtttaatcg
      481 cttcggcttt accgtccggc agcaccccgg ctatcacctc atcaatcccg gcttctttgg
      541 cgatcgcatt ggcggtggtt gggttatccc cggtcaacat caccagacga tatcccgctt
      601 tatgcagacg ttgcagcgcc gccacgctat cactacgcaa cggatcgcgt acagccagca
      661 gggctaccgc tttcccgtca accgccagca gcacaggcgt tgccccttgc gatgcctgag
      721 ctgaaatatc tgcttcaatc gctttagtat caacttgctg gtcatttaac agcgcctgat
      781 tgcccagcaa taacgcatga ccttcagctt caccgctcac gcccagcccg cgcaatgtgc
      841 ggaaaccgtt gacctgcggc agctgcatat cgcttgcttt atcgaggatc gcccgtgcca
      901 gcggatggct ggaaccttgc tccagcgccg ccgccagacg caatgcctgc gcttcatcaa
      961 catcagcaaa tgttttcact gcgacaacct gcggcttccc ttcggtcagc gtcccggttt
     1021 tatcgaacac gacggtatcg agtgtactgg cgcgttgcag cgcgtcggcg tcccgcacca
     1081 gcacgccaaa ctcagccgcc cgcccgacgc cggaaataat cgacatcggc gtcgccagcc
     1141 ccagcgcaca cggacaggca ataatcagca ccgtggtggc aatcaccagg gtatagacaa
     1201 tctgcggtgc cggaccaaag aaataccaga ttgccgcact gatcagcgca atcaccacta
     1261 ctaccggcac aaatacggct gagattttat ccgccagctg accgatttct ggcttgctgc
     1321 tctgggcctg gcgcaccata cgaataatcc ttgataacgt ggtatggctg ccaaccgcac
     1381 tggcgcgaaa cagcacgctg ccgtcctgaa ctactgtccc tgcatggacg ctatcgcctt
     1441 cgcctttttg ctgcgggata ggttcgcccg tcagcatcgc ttcatccagc catgcttcgc
     1501 cctgggtaat ctcgccatct actggcacgc ggtcgcccgt cgtcaggcgc agtagcatgc
     1561 ctggctgaac ttccgccagc ggcacgcttt tttcaccttc gtcggtgacc aggcgcgccg
     1621 tcggcggagt taaatcgagt aatttctcca gcgcctttga agaacgctga cgtgcgcgcg
     1681 cttccagcat atgaccgaga ttaatcagac cgataatcat cgcactggct tcatagtaaa
     1741 gatgccgcgc ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc
     1801 acgccacgcc agtacccagc gccaccagtg tatccatcgt cgctgcaccg ttcagcaggc
     1861 ttttccatgc gctgcggtaa aaatggccgc cggcgaaaac catcactgcc agggttatca
     1921 ggccgataac caaccacagg ctgcggttgt cagcggtgac catcatgtta tcgccgatca
     1981 tcccccagac cattaccggg atacccacag ccagtgcaac aattgcctgc cagcggaagc
     2041 gcttcatcgt agcgacggcg gtttcttgct ggcgctcgcg gcgtttcgcg tcatcttcaa
     2101 tcgcttccgc accgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac
     2161 tgcccatcac cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta
     2221 cgctttgcaa cgcattttgt acgcgggtga cacagctggc gcagctcatg ccgctcagca
     2281 gcaactgctg gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg
     2341 tcagtgcttc cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag
     2401 atgcgtcata acccgcttgt ttgatggttt cgatcagctg ttctgcactg gcagtcccgg
     2461 taacgtgcgc tttagtgata gacacatccg cttgttccac atccgggcgc tgttcgagac
     2521 tttctttcac gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc aggtcgatag
     2581 tttgtgacat aaaacactcc tttaagacag ttttgactgg ctgtgataaa ggttaaacct
     2641 tccagcaagg ggaaggtcaa gaaattaata acccaggcga gtaaaagtcc gcaaagatta
     2701 aaaaatcggc tcgatttgca tcaggattag acatttatct ctttgttttc ctgtagttaa
     2761 gttgtgggtg ctaagttaaa tcaggatgcc tgaaaatcgg caccggggtg aggaattacc
     2821 tcccgcatct ataaaaagga gttaacaaaa gatgttagat gcaaacaaat tacagcaggc
     2881 agtggatcag gcttacaccc aatttcactc acttaacggc ggacaaaatg ccgattacat
     2941 tccctttctg gcgaatgtgc ctggtcaact ggcagcagtg gctatcgtga ccagcgatgg
     3001 caacgtctat agtgcgggtg agagtgatta ccgctttgcg ctggaatcca tctcgaaagt
     3061 ctgtacgtta gcccttgcgt tagaagatgt cggcccgcag gcggtacagg acaaaattgg
     3121 cgctgacccg accggattgc cctttaactc agttatcgcc ttagagttgc atggcggcaa
     3181 accgctgtcg ccactggtaa atgctggcgc tattgccacc accagcctga ttaacgctga
     3241 aaatgccgaa caacgctggc agcgaatttt acatatccaa cagcaactgg ctggtgagct
     3301 ggtagcgctc tctgacgaag tcaaccagtc ggaacaaaca accaacttcc ataaccgggc
     3361 cattgcctgg ctgctgtact ccgccggata tctctattgt gatgcaatgg aagcctgtga
     3421 cgtgtacacc cgtcagtgct ccacgctcat caatactgtt gaactggcaa cgcttggcgc
     3481 gacgctggcg gcggggggtg tgaatccgct gacgcataaa cgcgttcttc aggccgacaa
     3541 cgtgccgtac attctggccg aaatgatgat ggaagggctg tatggtcgct ccggtgactg
     3601 ggcgtatcgt gttggtttac cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt
     3661 ccctggagtg atgggaattg ccgcgttctc accaccgctg gacgaagaag gcaacagtgt
     3721 tcgcggtcaa aaaatggtgg catcggtcgc taagcaactc ggctataacg tgtttaaggg
     3781 ctgatcatga tgaacacgga aggtaataac ggtaacaaac ccctcggtct atggaatgtc
     3841 gtttccatcg gtattggggc aatggtgggg gcggggatct tcgcgctgct ggggcaggct
     3901 gcgttgctaa tggaagcctc gacctgggtc gcctttgctt ttggcggtat tgtggcgatg
     3961 ttttccggtt atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg cggcattatc
     4021 gacttctttc gtcgcggatt aggcaacggt gtcttttcgc tggcgctctc gttactgtac
     4081 ctgttgacgc tggcggtgag catcgccatg gtcgcccgtg cttttggcgc ttatgccgtg
     4141 cagtttttgc atgaaggcag ccaggaggag caccttattt tgctctacgc gttggggatc
     4201 attgcggtga tgacgctttt caactcctta agcaaccatg cggtagggcg gctggaagtg
     4261 atcctcgtcg gcattaaaat gatgatcctg ttattgctga ttattgccgg tgtctggtcg
     4321 ctgcaaccgg cgcatatttc cgtctctgcg ccccccagct ccggtgcgtt cttctcctgt
     4381 atcgggataa ccttccttgc ctatgcgggc tttggcatga tggcgaacgc ggcggataaa
     4441 gtgaaagatc cgcaggtcat tatgccacgg gcgtttctgg tggcaattgg cgttaccacg
     4501 ttgctttata tctcgctggc gctggttttg cttagcgatg tatcggcatt agagttagaa
     4561 aaatatgccg ataccgccgt agcgcaggca gcttctccgc tgctcgggca tgtgggttat
     4621 gtgatcgtcg tcatcggcgc tttactggcg acggcttcag ccatcaacgc gaacctgttc
     4681 gccgtgttta acatcatgga caacatgggc agcgaacgcg aactgccgaa gctaatgaat
     4741 aaatccctgt ggcggcagag tacctggggc aacatcattg tcgtggtgtt gattatgctg
     4801 atgacggcgg cactaaattt aggctcactc gccagcgttg ccagcgccac ctttttgatt
     4861 tgctatctgg cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca cgcctcgttg
     4921 ccgattctta tcgtaggtac gttggtgatg ttgttggtga tcgttggctt tatctacagc
     4981 ctgtggtccc agggcagccg tgcgttgata tggattattg gtgcaatctt actcagcctt
     5041 attgtggcaa tggtcatgaa gcgcaataaa accgtataac acctctctgc gcgcagtact
     5101 gcctgtactg cgcctttgtc tttaattcaa attacctttc ttattccttt aataaataag
     5161 tcacaaaaaa atgatatata cgcgataaat ctgtgatgct aatcacttat taccgtggat
     5221 tattcttaat gtgtatgatg gtaatctaaa agtcgtagtg tattctaaat aaataacgta
     5281 taagcttt
//
LOCUS       DAZFFG010000208         5234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFFG010000208 DAZFFG010000000
VERSION     DAZFFG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.209"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(118..1338)
                     /gene="fsr"
                     /locus_tag="ACHJ7J_004792"
     CDS             complement(118..1338)
                     /gene="fsr"
                     /locus_tag="ACHJ7J_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO5002896.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            1556..3208
                     /gene="ushA"
                     /locus_tag="ACHJ7J_004793"
     CDS             1556..3208
                     /gene="ushA"
                     /locus_tag="ACHJ7J_004793"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5002897.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3245..3724)
                     /gene="ybaK"
                     /locus_tag="ACHJ7J_004794"
     CDS             complement(3245..3724)
                     /gene="ybaK"
                     /locus_tag="ACHJ7J_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO5002898.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3929..4723)
                     /gene="ybaP"
                     /locus_tag="ACHJ7J_004795"
     CDS             complement(3929..4723)
                     /gene="ybaP"
                     /locus_tag="ACHJ7J_004795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO5002899.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QVMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4861..5202
                     /gene="ybaQ"
                     /locus_tag="ACHJ7J_004796"
                     /pseudo
     CDS             4861..5202
                     /gene="ybaQ"
                     /locus_tag="ACHJ7J_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
BASE COUNT         1422 a         1249 c         1372 g         1191 t
ORIGIN      
        1 aatggaatta gctgcaaaaa aagcacggat cggaaaacag aaggcgattt aacagaggaa
       61 aggcttatgg cgactgtaaa acgatgcagc caaagtttgc ctttggctgc aatgaaatca
      121 gtctttatgc cggttatcag gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc
      181 acatatttta tagactaact cgatgctggt gtgatcggcg ataagcccca gaactgccgc
      241 tcccagacct cccatgccaa aagcaaaacc gaaaaagagt ccagaaacca taccgatacg
      301 tcctggaagc agctcctgag cgtagaccag aatggcagag aatgccgaag cgaggataaa
      361 tccaataatc accgttaaaa cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt
      421 aaacggcgca acgccgagga tagagcccca aatcacatat ttccgcccaa ttttatcccc
      481 tacaggcccg ccgatcaccg tacctgccgc aacggcaaac aggaaggcaa acagatgaag
      541 ctgagcattc tggatagata atccgaattt ttgcatcaga taaaaggtgt aatagctgct
      601 gatgctcgcc atatagaaat atttcgagaa aatgaggatt aacagaatac tgaccgccag
      661 tacaacttta ttgcgcggca gtggattgat aatcgtcgct ttgggttttc ctttattcat
      721 tcggtgctgt gccgagtacc aacggctgat ttgcgccaac accacgatcg ccagcagtgc
      781 cgcaagcaca aaccaggcaa cgttgccttt gccataaggc gcgataatca ccgccgccag
      841 caagggtccc agggaactgc caaagttgcc gccgacctga aagatagatt gtgccaggcc
      901 atgccgcccg ccggacgcca tacgggccac gcgagaagat tccggatgaa agaccgatga
      961 accggtaccg accagcgccg ccgccagcag aactgcgcca aaactgcccg ccagcgcaag
     1021 cagcaccaga ccacttaagg taaagcacat gccaattggc aacgaccacg gcatcggata
     1081 tttatcggtc cagtagccga ccactggttg cagtagcgaa gaggcgagct ggaaggtgag
     1141 ggttatcatg ccaatctgca taaatgtcag agaaaattct gactgaagca gcggataaat
     1201 cgccagaatc agcgattgga tcatatcgtt cagcagatgt gagaggctga tagcacctaa
     1261 aataccaaac gatgttcggg ccttggtcgt tgacgcagcc gcgcccgcca caggctgggt
     1321 ttgttcactc attgccatag gaaagtcact ttttcagggt tgcgatgtaa agaatgatct
     1381 tatttgtgat tattacccga ctaacatacc tgtatgcgcc gcctgaagga agtctcaacg
     1441 ctgaatacag aatttctaat ctggatgcag atttatcttc accggacgca gacttgtcta
     1501 tgatgtcgcg tcatactatt tttcaacacg ttgaaatcag gtcagggaga gaagtatgaa
     1561 attattgcag cggggcgtgg cgttagcact gttaaccaca tttacactgg cgagtgaaac
     1621 tgctctggcg tatgagcagg ataaaaccta caaaattaca gttctgcata ccaatgatca
     1681 tcatgggcat ttttggcgca atgaatatgg cgaatatggt ctggcggcgc aaaaaacgct
     1741 ggtggatggt atccgcaaag aggttgcggc tgaaggcggt agcgtgctgc tactttccgg
     1801 tggcgacatt aacactggcg tgcccgagtc tgacttacag gatgccgaac ctgattttcg
     1861 cggtatgaat ctggtgggct atgacgcgat ggcgatcggt aatcatgaat ttgataatcc
     1921 gctcaccgta ttacgccagc aggaaaagtg ggcaaagttc ccgttacttt ctgccaatat
     1981 ctaccagaaa agtaccggcg agcgcctgtt taaaccatgg gcgctgttta agcgtcagga
     2041 tctgaaaatt gccgttattg gtctgacgac ggatgacaca gcgaagttag gcaacccaga
     2101 aaacttcacc gatattgagt tccgtaagcc cgccgatgaa gcgaagctgg tgattcagga
     2161 gctgcaacag acagaaaagc cagacattat tatcgcggcg acccatatgg ggcattacga
     2221 caatggtgag cacggctcta acgcaccggg cgatgtggag atggcgcgcg cgctgcctgc
     2281 cggatcgctg gcgatgatcg tcggtggtca ctcgcaagat ccggtctgca tggcggcaga
     2341 aaacaaaaaa caggtcgatt acgtgccggg tacgccatgc aaaccggatc aacaaaacgg
     2401 catctggatt gtgcaggcgc atgagtgggg caaatacgtg ggacgggctg attttgagtt
     2461 tcgtaatggc gaaatgaaaa tggttaacta ccagctgatt ccggtgaacc tgaagaagaa
     2521 agtgacctgg gaagacggga aaagcgagcg cgtgctgtac acccctgaaa tcgctgaaaa
     2581 ccagcaaatg atctcgctgt tatcgccgtt ccagaacaaa ggcaaagcgc agctggaagt
     2641 gaaaataggc gaaaccaatg gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca
     2701 gaccaatatg gggcggttga ttctggcagc acaaatggat cgcactggtg ccgactttgc
     2761 ggtgatgagc ggaggcggaa ttcgtgattc tatcgaagct ggcgatatca gctataaaaa
     2821 cgtgctgaaa gtgcagccat tcggcaatgt ggtggtgtat gccgacatga ccggtaaaga
     2881 ggtgattgat tacctgaccg ccgtcgcgca gatgaagcca gattcaggtg cctacccgca
     2941 atttgccaac gttagctttg tggcgaaaga cggcaaactg aacgacctta aaatcaaagg
     3001 cgaaccggtc gatccagcga aaacttaccg tatggcgaca ttaaacttca atgccaccgg
     3061 cggtgatgga tatccgcgcc ttgataacaa accgggctat gtgaataccg gctttattga
     3121 tgccgaagtg ctgaaagcgt atatccagaa aagctcgccg ctggatgtga gtgtttatga
     3181 accgaaaggt gaggtgagct ggcagtaatc cgaaagtgcc ggatgttcgc atccggcaca
     3241 atgcttaatc gcggcgggcg atatcagcaa atttggcatc gaggatcttc gccagatcgc
     3301 ctgccgccag ttcgatatcc agtccacgct tgccgccgga aacataaata gtggcaaatt
     3361 cttgtgcggg ggcgtcgata atcgttggca gacgtttttt ctgccccagt gggctaatcc
     3421 ccccaaccag gtatcccgtc gaacgctgcg cgaccatcgg atcggccatc tcaactttct
     3481 tggcacccag cgcttttgct acttttttaa ggtccagttg acctgcgacc ggcgtaacgg
     3541 ccacggcaag gtgtttcata tcaccgttca ctgccaccag cagcgttttg tagacctgat
     3601 ccggattcaa acctaatttt ttgacgactt catcgccaaa attggtttca gccggatcgt
     3661 gctcgtaggt atggatttga aacgaaatct tgtttttttc gagtaattta actgcgggtg
     3721 tcatggtaat tttcctgcct taaacaaaaa aaacgaagca agcatacgcc tgatggcggt
     3781 ccaaaaaaaa gagtcatctt gcctaagagt attggcagga tggtgagatt gagcgacaat
     3841 cgagttacac cgtcgcttaa agtgacggca taataataaa aaaatgaaat tcctctttga
     3901 cgggccaata gcgatattgg ccattttttt agcgcaacat ttgcggcaaa ttcccttctc
     3961 catacaggtg tagtgcaccg accgcgacca catatcgccc cggcggcatg gcgcgtaatt
     4021 tatcccgcca ggcgagattt cgctgatgca tcagcacatc gtacagcgac tgactgaacg
     4081 tattgggcag cgttatttca ttattttgcg gcggcgcatt cagccaccag ctcatcattt
     4141 gttgcagcaa ccgtgcgttg gtatgccagt gggtcagcgt atcgtccagc agcgccagtc
     4201 ctttgtcagg gagctggagc aacatggcaa tctggttttc agccccttcc agttcaatca
     4261 cgggtttatg ttgttgcttc gccgcctgca ataactggta atcgataccg tattctgccc
     4321 gcagccccag tttttgtgcc tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt
     4381 gggtagaaaa gagtgacgga gaaatgccca tcacctggct aatgtgctgc agattttgta
     4441 attgctcctc gctaatacgc tcttccagcg cctcgcaggc aggcaaatta gcaaaaggcg
     4501 tatcgctggt ggaaacatcc gcctcgacga tcagcgcatc ggcgtttttg agctttttga
     4561 gtaaacgggt gggcagggga gccatatcgt ggctacccat atgaatactg ccaatcagat
     4621 gaaaatggcg attgccggga agggtgatat cgatggcagg ccaggtgtaa tgattaccgc
     4681 gcagcgcggc ccaaagcgtt tttacccggt acaacagatc catacgacct ccctttgtga
     4741 aatatcatgc tagcgcgcgg tgagggatgg cgcaaatgga gttggggact tgtttaatgt
     4801 gtttgtatga ttcagtatgt tcttgtatcg ctattcacag ggaagcaaca gttaaaaact
     4861 atgaaacagg caacaagaaa accgacgaca cccggagata ttcttctcta tgaatatctg
     4921 gaaccgctag atttgaaaat caatgagtta gcatagttgc tgcatgttca tcgtaatagc
     4981 gtcagtgcac tgatcaataa taatcgtcaa ctcaccactg aaatggcatt acgtctggcg
     5041 aaagtttttg ataccacagt cgatttttgg ctaaacctcc aggcggcagt tgatctttgg
     5101 gaagttgaaa acaacatgcg tacccaggaa gaattgggac ggattgaaac agtggctgaa
     5161 tatttggcac gccgtgaaga gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca
     5221 tctcgtaggc cgga
//
LOCUS       DAZFFG010000209         5207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFFG010000209 DAZFFG010000000
VERSION     DAZFFG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.210"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            215..1066
                     /locus_tag="ACHJ7J_004797"
     CDS             215..1066
                     /locus_tag="ACHJ7J_004797"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5002900.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLITFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1093..2082
                     /locus_tag="ACHJ7J_004798"
     CDS             1093..2082
                     /locus_tag="ACHJ7J_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5002901.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2113..3006)
                     /locus_tag="ACHJ7J_004799"
     CDS             complement(2113..3006)
                     /locus_tag="ACHJ7J_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5002902.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLRKYPEISVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINIRLPTAGGLYHWEFEREGKALRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYITSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3207..4133)
                     /locus_tag="ACHJ7J_004800"
     CDS             complement(3207..4133)
                     /locus_tag="ACHJ7J_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5002903.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            4290..>5207
                     /locus_tag="ACHJ7J_004801"
     CDS             4290..>5207
                     /locus_tag="ACHJ7J_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5002904.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT         1413 a         1273 c         1197 g         1324 t
ORIGIN      
        1 ggtatatacg ccaggataaa ccgatatatt ttttatggat attcattttc cttcatcgga
       61 ctgtaaattg cggagatatc gaacttgcac tacccatcgc aacactcaaa cagatttatg
      121 aatttcgttc ataacccctg acaaatcctg ccgtctaatc gagcttttcc ctttacgtta
      181 tgcttctcat gaacgagaac ggaaggataa caccatgcaa aaagtaaaac tgaacaacgg
      241 tattgaaatg cccctgttgg gctttggtgt atttcagatg accgatgccg ctgaatgcga
      301 aagagcggtc attgacgcta ttaacaccgg ttatcgactg attgacaccg ccgcctccta
      361 tcaaaatgaa acccaggtcg ggaacgcact gaaacaaagc ggtatcgccc gtaacgaact
      421 ctttgtcacc acgaaactct ggttgcagga taccagttac gaaggggcca aagcccagtt
      481 cgaacgctct ctgaatcgac tgcaacttga ttacgttgac ctgtatttga ttcatcagcc
      541 ttacggtgat gtccacggtg cctggcgtgc catggaggag ctgcaacagg ccggtaaaat
      601 tcgcgctatt ggcgtcagta acttccatcc tgaccgactg gctgacctta tcacctttaa
      661 taacgtcgtt ccagcggtaa accaggttga agttaaccct ttcaatcagc agctacacgc
      721 cgttccgtgg atgcaaagcc gtggtattca gccagaagcc tgggcaccgt ttgcggaagg
      781 gaaaaatggt ctgttccagc atcctgttct cacggcaatt ggcgagaagt atggcaaaag
      841 cgtaggccag gttgttctgc gctggatcta ccaacgaggc attgtttcgc ttgcaaaatc
      901 agtacgcaaa gaacgcatgg aagagaacat caacattctc gattttgaac tcagtcctga
      961 agatatgctg caaattaccg ccctggatac cgcaaccagc gcgttcttct ctcaccgcga
     1021 tcccgcaatg gtggaatggc tgactggccg caaacttgat gtttaagccg cgataaaaga
     1081 ggtattcatt caatgcaaaa acgttatctg ggtaaatccg gactcgaagt ttccgccctt
     1141 gggctcggtt gcatgggctt aagccacggc tacggcccgg cgaccgatac ccgtcaggct
     1201 atcgaactta ttcgtgctgc ggttgaacgt ggcgtcacct tcttcgatac cgccgaagtg
     1261 tatggtccgt atctgaatga agaagtggtc ggcgaagcct taaaaccgtt tcgtgaccgc
     1321 gtggtgatcg ccactaaatt cggttttacc ttcggcgatg ataataagca gcagatttta
     1381 aacagccgtc cggaacatat ccgtgaagcc gttgaaggat cattacgtcg tctgaagaca
     1441 gatgttattg acctgctgta ccaacaccgt gttgatccgg atgttcctat tgaagatgtt
     1501 gcaggaacgg tgaaagacct catcgcagaa ggtaaggtca aacatttcgg tctgtccgag
     1561 gctggcgcac aaaccattcg tcgtgcgcat gcggtacaac ctgtcaccgc cctgcaaagc
     1621 gaatattcca tgtggtggcg tgagcctgaa caggaaattc tgccgttgct ggaggaactg
     1681 gggattggtt ttgtcccctt cagcccacta ggtaaaggtt tcctgaccgg ctcgattaag
     1741 ccgggaacca ctttcggcaa agatgattac cgcagcaccg taccgcgttt cgcagcacaa
     1801 gccattgaag ctaatgaaaa actggtcacc ttgttaggtg aactggctgc ggagaaaggg
     1861 gtaacgtctg cacaaatcgc actggcatgg ctgttagcac aaaagccgtg gattgttccc
     1921 atccctggca ccactaaact gcaccggctg gaagaaaacc tggccgctgc tgacattgtt
     1981 ctttcgcaga aagatacgca gcagataacc gaggcgctgg aaacaattaa aatcgtcggt
     2041 gaacgttact cgcctgaaca ccaggcacgc gtaggccgct aatacccaca cgggtcctgg
     2101 acaaaggacc cgttattcgg tgtagcgaag cgcatcgatc aacagcgcaa aagcaggcgg
     2161 atgctgcttg cggctggggt agtagagata atatccggga aaagttggac accactcctg
     2221 caacacctga ataagcttgc ctgacgttat atactcctga accatgtctt cagggacaca
     2281 agcgatccca aaccctgata acgcggcatc aattctttct ggcaatggac tacacgtcag
     2341 ctgcccatca accttaacgc gaagcgcttt cccttctcgc tcgaattccc agtggtaaag
     2401 tccaccggca gttggcaaac gtatgttgat gcagcgatgg ttttgtagct cgtgaggcgt
     2461 ttcaggcgca gggtttgcag caaaatacgc cggtgccccc accacagcca tccgcatatc
     2521 tggtccaatt ctgaccgcaa ccatatcttt atcaacattt tcacccagtc gtatcccggc
     2581 atcaaaacgg ccttcaacaa tatcgacaaa accgttatcc accaccagtt cgacactgat
     2641 ttcgggatac tttctgagaa aaggttttag cttcggccag accagacttc gcgcggcatg
     2701 ttccccggca gataaacgaa tattcccgga gggcgtgccg ttctgttgaa taagtgactc
     2761 cagttcctgt tcaagctcgt caatacgcgg ttcaaggcag gcaatgattc tctcccccgc
     2821 ttctgtggga gccacactgc gggtagtccg tgtcaggagg cggatattga gcctttcctc
     2881 cagagccttc attgcatggc taagtgcaga ctgagagacg ccgagtttgc ccgctgcttt
     2941 ggtaaaactt cgctctcttg ccaccacaag aaagatttgc agttcgttga agttttcttt
     3001 gagcattggc gatttccttg tagaaacatc ctcttcacag acactcatga agggggggca
     3061 tagatgctat tattttagcc acattattag taacaatgat aatctggctg tatcgaaaat
     3121 aattctaaac tttcaggaga tttattatga ataaacgtat cctcacgatt gtgccattct
     3181 tcttaccgat attctgacac atatcatcaa ttttcgagtt ttactctttt ctccaccata
     3241 aaatcaataa aagcacgaac tttagagggc attctcaccc gtgatgaaac ataaatataa
     3301 aatccaggaa atgaaacagc ccaatcctca agaatcggct gtaacttgcc cgcattgata
     3361 tgttcagcga ttgcaaaatc cagatgttga atgatacccg ttccattcag cgccattcgt
     3421 atcatgcttt cgtcatcatt agaaatatac ttacctgaaa cctctatttc gaactcttca
     3481 ttacccccca caacattaga aaactcccat gcagagagtg caccgctact tttatgccgc
     3541 aaccgaaggc aattatggtt actcagttcc tgaggcgttt caggaatacc gtgttctttt
     3601 aaataatctg gcgatgccac agtaaccagt ttaataagcg gagaaacagg aacggcagtc
     3661 atatgctcat ccagtccttg ctcaagacgt atgcccacat cacagccgtc cgcaataatg
     3721 ttagataaac cgtcatccat aatgagttct atctttgttt tggggtaacg agtaagaaac
     3781 tcgccaatat gaggctcaac aagcatccgt gctgcaacac gggaggaatt tattctcaac
     3841 aggcctgtag gttcaagttg ggtatcacca acatctttca aagcatcatc aatttgcccc
     3901 aaagccgaca ccagcacttc atacagacgc tgtccttcct cagtaagcga catattgcgg
     3961 gtcgtacggt atatcagctt ggtgttaaga cgttcctcaa gagattttaa attctgggac
     4021 aatgcggccc gcgtaatacc catttcagaa gcggctctgg aaaaactgcc ggcacgaaca
     4081 atcagcgcaa accaggcaag agaaggcaaa attgaaggct ctattttcat cattgtaaac
     4141 cttaacttaa cactgtgata gatattagac cattgatgat aaccaataca agtcatacta
     4201 ttacgccgtt ctcgatatga acacgaatgc cagttgcgga taatagaaat gatatttaaa
     4261 tctgtttagg caaatccccg gagcttgaaa tgaaaactgt ttcgataaaa cacacttact
     4321 gggatatagc tgctgatatc tatttccctg acgattttaa tagtgaaaaa aagtatcctg
     4381 ccattatcag cgcccatcca attggaagct gcaaagaaca gacatcgggt agtgtgtatg
     4441 gtgccgccct ggcaaaagct ggctttatcg tcattgcttt tgatgccagt tttcagggaa
     4501 gcagcggtgg ggaaccgcgt tatctggaag atccaacaat gcgagtaaaa gacttcagca
     4561 ttgtcgttga ttaccttacc agcttacctt acgttgatgc cgggcgtatt ggtgtactgg
     4621 gtatttgtgg tggaggtgga tatgccatta atgccgcaat gacagaacgt cgaattaaag
     4681 ctattggtac tgtcacgggt gcaaactacg ggcgtcttat gcgtgaggga tttacagcgt
     4741 ttaatcctat cggagcactc gaagcaatgg cgcaacaaag aacagacgaa gctaacggcg
     4801 caaaacttcg cgtagatgat ttactcccct cttctccgga tgcagctctt gaagcgggat
     4861 taacagaaat agaccttttt gaagccacag aatattatcg ttcgccacga ggctatgcac
     4921 ctaatggggt aaatcgttcg ctattctcac atcagactgt tgccgtgggt tgggatgcat
     4981 ttcatcttgc agaagtcctg ttaactcaac caataatggt tgtggtgggc gatagagtgg
     5041 gcgcatttgg tgcctaccgt gacggctgcg aaattattgg ccgcgctgct tcaaaacata
     5101 aagagctggt cgtggttgaa ggttattcac attacgacct gtatgataag ccagaacctg
     5161 tgaaacaggc actcgataaa ttaattcctt tttataaaac acatttg
//
LOCUS       DAZFFG010000210         5183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFFG010000210 DAZFFG010000000
VERSION     DAZFFG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.211"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            81..797
                     /gene="nanC"
                     /locus_tag="ACHJ7J_004802"
     CDS             81..797
                     /gene="nanC"
                     /locus_tag="ACHJ7J_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO5002905.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            817..1923
                     /gene="nanM"
                     /locus_tag="ACHJ7J_004803"
     CDS             817..1923
                     /gene="nanM"
                     /locus_tag="ACHJ7J_004803"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO5002906.1"
                     /translation="MNKTITALAIMMASFAANASILPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGTRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1988..2968
                     /locus_tag="ACHJ7J_004804"
     CDS             1988..2968
                     /locus_tag="ACHJ7J_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5002907.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLVNIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(2976..3626)
                     /locus_tag="ACHJ7J_004805"
     CDS             complement(2976..3626)
                     /locus_tag="ACHJ7J_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5002908.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIDQGFHIHQDKAAAAPIADVLFPD
                     ELPSQGTYVEGAAIKVTVNSYERDKKAREKAVEYHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYLLDPVNDLLPVCPNCHAMLHRRKPPFMPEELKALMDANKSN"
     gene            3763..4635
                     /locus_tag="ACHJ7J_004806"
     CDS             3763..4635
                     /locus_tag="ACHJ7J_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO5002909.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLHGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAERCSTAGGSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYKYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVEWHRKEVFRG"
     gene            4680..4859
                     /locus_tag="ACHJ7J_004807"
                     /pseudo
     CDS             4680..4859
                     /locus_tag="ACHJ7J_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         1492 a         1128 c         1205 g         1358 t
ORIGIN      
        1 aaaaatatta cacaacataa atgctaattg tttatgcggg ctttgtattg ctttctgtat
       61 cctacaaatg agtgaaattt atgaaaaagg ctaaaatact ttctggcgta ttattactgt
      121 gcttttcgtc accattaatt tctcaggctg cgacacttga cgtacgtggt ggatatcgta
      181 gtggaagcca cgcctatgag actcgactca aagtcagtga gggatggcaa aatggatggt
      241 gggcaagtat ggaaagtaat acctggaata ccattcatga taataaaaag gaaaatgccg
      301 cactcaatga tgttcaggtt gaagttaatt acgcgattaa acttgatgat caatggacgg
      361 tgcgcccggg aatgttaacg cattttagca gcaacggcac acgctacgga ccctacgtaa
      421 aactgtcctg ggacgcgaca aaagatctta attttggcat tcgctatcgt tacgactgga
      481 aagcttaccg acaacaagac ttatccggtg atatgtctcg tgataacgtt catcgttggg
      541 atggatatgt cacttaccat attaatagtg atttcacctt cgcatggcaa acgacgctat
      601 acagcaaaca gaacgattat cgctatgcaa accataagaa atgggcgacg gaaaatgcat
      661 ttgttctaca ataccatatg acgcccgata ttacgccata catagaatat gactacctgg
      721 accgtcaggg tgtttacaac ggcagagata atttatcgga aaacagttat cgcattggtg
      781 tgtcatttaa actgtagtag acaggagaca gtcacaatga ataaaacaat aacggcgctt
      841 gctatcatga tggcttcatt tgccgcaaac gcgtctatat taccggaaac tcctgtgcca
      901 tttaaaagtg gtaccggagc aattgataac gacactgtct acattggttt aggtagcgca
      961 ggtacggcat ggtacaagct ggatacacag gccaaagata aaaaatggac agcgttagct
     1021 gcattccctg gtggaacaag agatcaagca acctcggcat ttattgatgg caatctgtat
     1081 gtgtttggcg gcattggcaa aaatagcaag ggcttgactc aggtatttaa tgacgtacac
     1141 aaatacaacc ccaaaaccaa tagttgggtt aaattgatgt cgcacgcacc gatgggcatg
     1201 gcaggtcatg taacttttgt acacaacggc aaggcttatg ttactggcgg tgttaaccag
     1261 aatatcttca atggctattt tgaagatctc aacgaggctg gaaaagattc aaccgctata
     1321 gataaaatca acgctcacta ttttgacaaa aaagcagaag attatttctt caataagttt
     1381 ctgttgtctt ttgatccctc aacacagcaa tggagttacg ctggcgaatc tccctggtac
     1441 ggaacggctg gtgcggcggt tgtgaataaa ggtgataaaa cctggcttat taatggcgaa
     1501 gccaaaccag gattgcgaac ggatgccgta tttgaacttg atttcaccgg taataattta
     1561 aaatggaata agcttgctcc cgtctcatca ccagatggcg tcgctggcgg ttttgcgggg
     1621 ataagcaatg attctcttat atttgccgga ggggccggat tcaaaggttc acgagaaaat
     1681 taccagaacg gtaagaacta tgcgcatgaa ggcctgaaaa aatcatatag cactgatatt
     1741 catctttggc ataacgggaa atgggataaa tcgggtgaat tatcgcaagg tcgggcctac
     1801 ggagtatcat tgccctggaa taatagtcta ttgattattg gcggtgaaac tgcaggcggc
     1861 aaagcggtga cggattcagt tttgatctct gtgaaggata ataaagtcac agtacaaaac
     1921 taacgcttca gggccccagt aaaggggccc tgctatcaat ttatcatttc aattacgcga
     1981 aaattatatg gacgcaataa tatcgcccga ttattactat gttcttaccg ttgctggtca
     2041 gtctaatgct atggcgtatg gcgaaggact gccattaccg gacagggaag atgcgcctca
     2101 tcccagaatt aaacaattag cgagatttgc gcatacgcat cccggaggcc cgtcatgtca
     2161 ctttaacgac attattccac tgactcactg cccacacgat gttcaggata tgcagggtta
     2221 tcaccatcct ctggcaacga atcatcaaac acagtacggc accgttggcc aggcactgca
     2281 tattgcacgg aaattactgc cctttattcc tgataacgca ggggttctca tcgttccgtg
     2341 ttgccgtggc ggatcggctt ttaccgcggg cagcgaaggg acatattcag aacggcacgg
     2401 agccagccat gatgcttgtc gttggggaac ggatactccg ctataccagg atttagtcag
     2461 cagaacgcga gccgcattgg caaaaaatcc gcagaacaaa ttcctcggcg tatgctggat
     2521 gcaaggcgaa tttgacttaa tgaccagtga ctacgcgtca caccctcaac actttaatca
     2581 tatggttgaa gcctttcgta gggatctaaa acaataccat tctcagctta ataatattac
     2641 tgatgcgccc tggttttgcg gcgataccac ctggtactgg aaagaaaatt tccctcatgc
     2701 gtatgaagct atttatggca attaccaaaa taatgtttta gtcaatatta ttttcgtcga
     2761 cttccagcaa caaggtgaaa gaggactgac gaacgcgcct gatgaagacc cggacgattt
     2821 aagcacggga tattacggtt cagcgtaccg gtcaccggag aactggacga cggcactacg
     2881 aagcagtcat ttcagcgcgg cagcccgtcg agggattatt tctgacaggt ttgtagaagc
     2941 aattttgcag ttttggcgcg aaaggtgagc gttcattaat ttgatttatt cgcatccatc
     3001 agcgctttta actcctccgg cataaacggt ggtttgcgtc ggtgcaacat cgcatggcaa
     3061 ttagggcaca caggaagtaa atcattaact ggatccagca ggtaatcctc tttaatggct
     3121 gaaagcggca ccaggtgatg catatggata aagccctcag cgatatcgcc atagatcttc
     3181 accagatcaa cgccgcatac gttgcactgg catccgtgat attcgactgc tttctcgcga
     3241 gcttttttat cgcgttcata gctgtttacc gtcaccttta tcgccgcacc ttcaacgtag
     3301 gtcccctgcg acggcaactc atccgggaac agaacatcgg ctatgggagc cgcagcggct
     3361 ttatcctgat gaatatgaaa tccctgatct ataagtagct tgacgcattt agattttatt
     3421 ccgccggtga aatccgcagg ggtaaattgc gtccccgtca tcaatgtcgc tgcgatgcca
     3481 gcaatcgcct tcgaggggta acgtcggcca tgatattcaa tctcatacaa ccgtgcaggc
     3541 tggaactgat gagtttctcc cgcatcccat aaatgaatag cttcgataat ataccgccgc
     3601 ggaagatgat cggggagttt attcatagag aatgtcctta tacgttctca gaacaagcag
     3661 caaaaaaaca aatgagatca aagtaatata tcttcacttg caggcttgaa cagcagaaaa
     3721 tttgcgagca tgatcgcaat tatttcactg ccagggatat caatggcttc cagtaaatcg
     3781 ctacagcagg caattgccaa tataaaaatc tggcataagg gtgaacagcg cgcgccgcat
     3841 aagccattgt tattgctata cgtattagcg ggatacctgc atggacatcc gcgccttttc
     3901 gattatggct cggaaatcta cgaacctctg cacagtttac tggaacgttt tgggccacag
     3961 cgttcacagt accgacctga tatgccgttc tggcgattac aaggcgacgg attctggcaa
     4021 ttacacaatg ccgagcgttg ctcaacagca gggggcagcc gacaaccgcc agtgaaggaa
     4081 ctgaacgaat accacgttgc aggcggtttc gatgaacagc actacgccct ggtaaccggc
     4141 aataaaaaac tcatcaatac tctggcgcaa cagatactcg aagcgcactt caccgagagt
     4201 attcaggaag aaattgctga cgagctgggt tttgatctcc agcaaatccg taaacagcgc
     4261 gacccgttat ttcgcaaaaa tgtattacgc gcttataaat accagtgcgc gatatgtggc
     4321 tttaatatgc gccacgacga caccaccgtc gcgctggaag cggcgcatat aaaatggaaa
     4381 cagcacggcg gtccttgtga aattcctaat ggcctggcgc tttgcgcaat tcaccataaa
     4441 gcctttgata aaggatcgat tggcctggac gaaaatatgc gggtactggt atcagatgcg
     4501 gtaaacggcg gagggatcgt tgagagatta ttctgggatt ttgacgggaa aactattgcg
     4561 ttgccgcagg tgcgtaaaaa ttacccttat gaggggtttg tggagtggca tcggaaagag
     4621 gtttttaggg ggtgaggttg ataactatcg cagtatgttc aatattgctg acataatcag
     4681 tgcaggatct tgccgctagt ttacaactcg ccttcgatag cggcgcaaaa cgagttctgt
     4741 tgccgatatc ctcggctatg gatattccaa cagttccggc agagttgttt accaagtttc
     4801 aggtaagttt ttactcagaa ccggttgatg cggtttataa ggcgctggat gtgaattaac
     4861 acattcctca gcttatttat aaaaggatta ttcccatttg atataatatc agtgccaatt
     4921 tacttttttc aatggaatat atctcggcta aataacaata aaacttatcg ctaatggatt
     4981 aaataaacaa ttttctaatt catttcacca cctttgatat agaaattgat ggtgttgtcg
     5041 ctgccattgc ttatggtgat gataaaatcc acgtttctgg atcgttgcgt agaaaatcat
     5101 cactaccttg atattttgat gcgatatgat cataccgttc cgtagtacca atttcaattt
     5161 caggctaaac cccaacatta aaa
//
LOCUS       DAZFFG010000211         5156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFFG010000211 DAZFFG010000000
VERSION     DAZFFG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.212"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1421)
                     /locus_tag="ACHJ7J_004808"
     CDS             complement(<1..1421)
                     /locus_tag="ACHJ7J_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFO5002910.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKRKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGC"
     gene            complement(1414..2559)
                     /locus_tag="ACHJ7J_004809"
     CDS             complement(1414..2559)
                     /locus_tag="ACHJ7J_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HFO5002911.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLNNALVTFPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            complement(2564..4267)
                     /gene="sctC"
                     /locus_tag="ACHJ7J_004810"
     CDS             complement(2564..4267)
                     /gene="sctC"
                     /locus_tag="ACHJ7J_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFO5002912.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIIIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(4264..5013)
                     /locus_tag="ACHJ7J_004811"
     CDS             complement(4264..5013)
                     /locus_tag="ACHJ7J_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5002913.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT         1571 a         1107 c          832 g         1646 t
ORIGIN      
        1 taacagccaa tttttgttaa tttttgagca tcacttttcg taacccaata atattgttca
       61 ccattactat ttacaatgac aggtttcttg atcaaagtcg aaaccacttc atctgaatat
      121 tcaaccaaca agacaagatc atgataaatt ttaaattgcg aagctctgac ttcatttagc
      181 attaatatga catcttcatc attaagacct tccccctcac gaatgacaat accgggaatg
      241 cggataccat actcaataaa aaactgacta cgaatcctat cagcaagatt gatttcagtg
      301 tattttttag cctgaacact attcataatc aagaccaagg gtaccgtttc ggtaataacc
      361 tgatcaagtt tattaatgat acctaatgag atgtcatcat cttgattata gggttccaac
      421 ccaacggtaa tatctccctc aacaactgtt tcttcagtcc ctttcctttt gaatttaaag
      481 taaaagtaaa ttcctaagat cactgccaaa cataaaaaca caagaagggg aaaacctgga
      541 agtaacccaa tacttagcgc gaggacacat gtcacaataa tgacaaaaga attacttaat
      601 aactgggaca ttatattttg ccccatatta tcactatcgc catttacgcg cgtaacgata
      661 aatcctgcgc taatagcaat taataaagct ggaatttgag aaacaagacc atcaccgaca
      721 gtaagaattg tgtagacagt caacgctgtt gagaaatcca ttccattttg gcctactcct
      781 actgatagcc caccgataat attaacaaag ataataataa tgtttgcaat agcatcacct
      841 ttgataaatt tcatcgcacc atcaaaggaa ccatacagct gactctctcg ttctaatacg
      901 ctacgacgtt cttttgctaa atctgcatca atgatcccag cgcgtagatc ggcatcaatg
      961 ctcatttgtt tacctggcat accgtcaagc gaaaagcggg cagcaacttc agccacacgt
     1021 tccgaacctt tagtaatcac gataaactga acaatagtaa caatggagaa aataacaaaa
     1081 ccaactacca ggctatctcc aataacgaac tcaccaaaac tcgttataat ttctccagca
     1141 tccgcctcaa gaagtataag tcgactggta ctgatcgcca gcgccaaacg aaataacgtt
     1201 gtgataagga ggattgatgg aaatgaagaa aagcttaaga tcctatcaac atagaatgat
     1261 cctaaaaata caagtattgc caatgttaag ttaagcccaa tcaaaaagtc gattaaatag
     1321 gtgggcaatg gaattatgag catggcgata atcatcacca ttaatccaag aattattaat
     1381 tctggatgac tccttaaccc taccaaaact ttgttaaaca ttgaacatca ccttcttcca
     1441 tggtttttta ttttgttttt tgttacagtt ttgtgtatca aagagctctg ctgcaaatgt
     1501 tacttttatt gcctgtttac aacactccct aatctctaat aattgttcat tattaataaa
     1561 aagtgaaaat ggaagatttg tacatccact gtaaatcgcc ttcaggagta acgttttgtc
     1621 tttataatta gcatctgcgg gaaaggtcac cagtgcattg ttcaggagtt cctctgcagt
     1681 ctcaggaaat tttattaacg atatcagaaa aaatatccag taatcttctg cgctgataaa
     1741 ttttttaatt attgggttat tcattaaagt tttcagaaat aaagcctctg ccgactgaag
     1801 agacaatagt tgtgacaact tattgagaaa cataccaaat tccaatgatg aacaactcgc
     1861 atcaagtgca tttatatctg agaaaagcgt ctctttgata aattttgtca ccaacattct
     1921 attttgatag ccataactac caatccattc gacataagta tctacagcat cgtcttctgc
     1981 cattaaaaat tgccgataac tcgcccttag taattttgga tttaatgaca gcgcctggcc
     2041 aaataacttt gcttttaatg ccgagttaat gccgctatta atgaattttt tagtagtctc
     2101 ttgattaact tttctaagta atgcttcagc gtttagccgg acattttctg atatttgttt
     2161 tttacgtaaa attgcacgta acacaactac cagatcgctt tcatctggga ataatttttt
     2221 agcaaagcta tacacttcat agtcatgggt agttgcagcc ttaagcgcct ccataacttt
     2281 gtcggttttt tctattggct ggtcttccag gacatattct tctccgtcca gaaaattggc
     2341 cagttgttct tcgtagattt ttttcttcat aaactgcgtt aacgctgctg acatctcgtc
     2401 agtagcttgt aagaaccgag cttctttacc tgcgggatca tcccccattt taggcatttc
     2461 agcttcaata tcttcattat cttctgtgat aatgtcctct aagcgagaaa cttccctggc
     2521 tctttctaat acaccgacat gctctacatg aatcgccatt tcactatcct ccgacgactt
     2581 cacgattcag gtaagttttt atccatttct gcagtaattc atcgttgatt tctttatttt
     2641 ttgccatttg ctgtgtaatc gctctggcat caaccgcagc ctcattcgca ttattcatca
     2701 tgcgttcatc gatctctctt ggctcaataa gaaagacacg aacgatgtta ttcgcatttg
     2761 ttccttcata accaaataat ttaccaataa atggaatgct gcctaatatt gggatcttgc
     2821 gactttcata ggtattcgta tcgcgtgtat atcccccaat caaaagactt ttcccttggg
     2881 gcacgcgtgc aatagtactt ataagtgttc taccaacctg aggcagatca tcaacattgg
     2941 tcttgtcgga attgatttca ttgccatctt caatattcaa gagtaattca atttgatttc
     3001 gcgcagcaaa tcgtggtagc acgctaatca tcgtaccata ggttacttca tccaattccg
     3061 cagtacgctc gccgactaat ttagtataga aagtgcggtt attatcaaat atagcaggaa
     3121 tattctcttg tgttaaaacg acgggacgag caacaacggc ggctcttctt ttttgtgcaa
     3181 gtgcctgtat tgttgcaata aactgtgtcc catcaagggt actaattgaa cctgaattat
     3241 taaatgatgc acttaagctg gatccaattt ttatcgtgcc cgaccagtca gcacctagct
     3301 gttctagatc agtcttatca atatctatta tccataaaga taattcaatg tgtcgctttg
     3361 gaatatcaag cgtagctact aacttctcga taaaatcaac ctgagatact gttcctttaa
     3421 caagtaaact gttggtatca ggatatgcaa taatttgtat atcgtcagtc tctggcgctc
     3481 ctgctgtcga tattattttc tctacttgta ggtcatcatc ttcttcagca ccaggaaaag
     3541 gaggcatacg tgatacgttt tttagttgtt gacgacttga aatttcagaa agtacgttca
     3601 cccctgtgct ttgcttaatg ttattattta acaacgtcga tagtactttt gccatgccag
     3661 gaataattat cttttcgcca cgtaactcat aagtccggtc attaacgaat gtgttgacca
     3721 gatgaattat tcctacttta tttcgaccga tctcaatgcc atcgctgttt tgctccatta
     3781 atttcgcagc attaacgacc aggtcaacat aaaccggtgg ccccgataca tagaaagtac
     3841 cattgccgcc ccctttaatt tcataacgag agttatacag acccgatttt tttaagaaat
     3901 tattaaactc attcgtcgat acctttctca gattaatcaa tgcattacgc atctctgatg
     3961 agtcatagat ataaatagct ttgccatcat cataccaaat aagccccatc tgtgccgaca
     4021 ggcgttcaat caaggcatag ggttctgtta aatcaaaatt tccgctgatc tgtttcttca
     4081 tggcaagctt actaactatt gtaggcttac ccgcatatga tgaaaccgtt tcaaaaaaac
     4141 tacgtagatt ttctttattt gctacgtatc cattcgctgc tgcggcatat gcaccaggag
     4201 tcaataatcc attaaaaatg cataagactg aaattaatat aatagtaatg cgtaatttta
     4261 ttttcataac tttttacata gttctctcgg tgtttttcct aaccggcttt ttacttcggc
     4321 tgaaaagtgt gatgaggacg agtaaccata tttatgcgca atagttgtca tatcactgtt
     4381 tccttcaatg atttccaaca cggctctggc aactctccag ccacataatt ccgttttaac
     4441 tttaccaccc aatgcataac tacataaacg gcgaaaatgt gaatacgata cgccgtaaag
     4501 ctcaccaaga tcatagagtg aagtctgtat gagcgattgc gataataaat aacgaaccag
     4561 tttatagcat tctgtttttc ttagatgctg aaacaggatt tcattaatat tatcgctgct
     4621 cagataagtg gctaaaaacc aggcttcact tgcaacttta ctgaatagca caacttctga
     4681 tgcaaagggc aaggcatcag caaacaattc atgatgttcc ctttccacac tccccgttga
     4741 ttcatctata aaagctaata atttactcag gatatcaatg cgaagtggat gataaattac
     4801 tttccctgca gttacctcaa tttggcttcg gtcaatgagc agtaatatat cttccactac
     4861 cattaatttt tgatggttat taccaatcgt aataacaagg gttacatcct tgttttcatt
     4921 tccccgtaag aaccacattt cagggtccag tagggttaac ttctgtcctt gaagcaaaac
     4981 attcattgga agtaacagcc cttcttcaat catgtttcat ctccttattc catattctca
     5041 ttctccataa acttgctata aaaacatgat taaaaagtga tgattactat ttataaaata
     5101 atgattttgt tatgagcgaa tagctatcag ccacttacac tagtacgtca tcctgt
//
LOCUS       DAZFFG010000212         5148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFFG010000212 DAZFFG010000000
VERSION     DAZFFG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.213"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            93..1427
                     /gene="garP"
                     /locus_tag="ACHJ7J_004812"
     CDS             93..1427
                     /gene="garP"
                     /locus_tag="ACHJ7J_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO5002914.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1443..2213
                     /gene="garL"
                     /locus_tag="ACHJ7J_004813"
     CDS             1443..2213
                     /gene="garL"
                     /locus_tag="ACHJ7J_004813"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO5002915.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2243..3133
                     /gene="garR"
                     /locus_tag="ACHJ7J_004814"
     CDS             2243..3133
                     /gene="garR"
                     /locus_tag="ACHJ7J_004814"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5002916.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3230..4375
                     /gene="garK"
                     /locus_tag="ACHJ7J_004815"
     CDS             3230..4375
                     /gene="garK"
                     /locus_tag="ACHJ7J_004815"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO5002917.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4408..4784
                     /gene="rnpB"
                     /locus_tag="ACHJ7J_004816"
     ncRNA           4408..4784
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHJ7J_004816"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5011..>5148)
                     /locus_tag="ACHJ7J_004817"
     CDS             complement(5011..>5148)
                     /locus_tag="ACHJ7J_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002918.1"
                     /translation="LEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYTYNYDLLNA
                     I"
BASE COUNT         1219 a         1221 c         1455 g         1253 t
ORIGIN      
        1 cgagttcatt gtcgttacgg cataaatgaa actcataaat aacaccttcc ccataataat
       61 aaaaatcagc ataagtaccc gaggtaaata aaatgattct ggacaccgtt gacgaaaaaa
      121 agaaaggcgt gcatacccgc tatttaatat tactgattat ttttattgtt accgccgtta
      181 actacgccga tcgtgcaacg ctgtctattg ctggtaccga agtggcaaaa gagttgcagt
      241 taagtgcggt ttcgatgggt tacatcttct ctgcttttgg ctgggcctac ttgctgatgc
      301 aaatccccgg cggctggctg cttgataagt ttggctcgaa aaaagtttac acctatagcc
      361 tctttttctg gtcgctattc accttcctgc aaggctttgt tgatatgttc ccgctggcct
      421 gggcagggat ctccatgttc tttatgcgct ttatgctcgg cttctcggaa gcgccatcat
      481 tcccggcgaa cgcccgtatt gtcgccgcct ggttcccgac gaaagaacgt ggtactgcct
      541 ccgccatctt taactcggcg caatatttct cgctggcgct cttttcgcct ctgcttggct
      601 ggctgacttt cgcctggggc tgggagcacg tctttaccgt tatggggggg attggttttg
      661 tgctgacggc gctgtggatc aaattgattc ataacccgac agatcaccca cgtatgtctg
      721 cggaagagct gaagtttatc tctgaaaatg gcgcggtggt cgatatggac cacaaaaagc
      781 cgggcagtgc ggcagcaagc ggacccaaac tgcattacat caagcaattg ctctctaacc
      841 gcatgatgct tggtgtattt ttcggacaat attttatcaa caccatcacc tggttcttcc
      901 tcacctggtt cccgatttat ctggtgcagg aaaaaggtat gtcgattctg aaagtgggtc
      961 tggtcgcctc gattccagca ctgtgtggtt ttgcgggcgg cgtgctggga ggtgtcttct
     1021 cggattatct gatcaaacgc ggtttatccc tgaccctggc acgtaagcta ccgattgtgc
     1081 tgggaatgtt gctggcttcc accatcatct tatgtaacta caccaacaac accacgctgg
     1141 tggtcatgct gatggcgctg gctttctttg gcaaaggatt tggtgcgctg ggctggccgg
     1201 tgatttctga caccgcgccg aaagagattg ttggcctctg cggcggcgtc tttaacgtct
     1261 ttggcaatgt tgcctccatt gtcactccac tggtgattgg ctacctggta agtgaactgc
     1321 actccttcaa tgcagcactg gttttcgtgg gatgttcagc gctgatggcg atggtctgct
     1381 acctcttcgt agttggcgac attaaacgta tggaattgca gaaataagca aaggtataag
     1441 cgatgaataa cgatgttttc ccgaataaat tcaaagccgc actggctgcg aaacaggtac
     1501 aaattggttg ctggtcagca ctctctaacc cgattagcac tgaagttctt ggtttggctg
     1561 ggtttgactg gctggtgctg gatggcgaac atgcgccaaa cgatatctcc acgtttattc
     1621 cgcagttaat ggccctgaaa ggcagcgcca gcgcgccagt agtaagagtg ccgaccaacg
     1681 agccggtaat tattaagcgt cttctggata tcggtttcta taacttcctg attccttttg
     1741 tagaaacaaa agaggaagca gagcaggcgg tggcatcgac gcgttatccg ccagaaggca
     1801 ttcgcggcgt ctccgtttct caccgcgcca atatgtttgg caccgtggcg gattatttcg
     1861 ctcagtcgaa caagaacatc actattctgg tccagataga aagtcagcag ggcgtagata
     1921 acgtcgatgc cattgccgct accgaaggtg tagacggcat cttcgtcggc cccagcgatc
     1981 tggccgcggc attaggccat ctcggcaatg catcacaccc ggatgtacaa aaagcaattc
     2041 agcacatttt taaccgtgcc agcgcgcacg gcaaacccag cggtatcctc gcgccggtcg
     2101 aagccgatgc gcgtcgttat ctggaatggg gcgcgacgtt tgtggctgtc ggcagcgatc
     2161 tcggcgtctt ccgctctgcc actcagaaac tggctgatac ctttaaaaaa taaccaccac
     2221 cgcaacaaga gagatgattg atatgactat gaaagttggt tttattggcc tggggattat
     2281 gggtaaacca atgagtaaaa accttctgaa agcaggttac tcgctggtgg ttgctgaccg
     2341 taacccagaa gctattgctg acgtgattgc tgcaggtgca gaaacagcgt ctacggctaa
     2401 agcgatcgct gaacagtgcg acgtcatcat aaccatgctg ccaaactccc ctcatgtgaa
     2461 agaggtggcg ctgggtgaga atggcattat tgaaggcgcg aagccaggta cggtattgat
     2521 cgatatgagt tctatcgcac cgctggcaag ccgtgaaatc agcgaagcgc tgaaagcgaa
     2581 aggcattgat atgctggatg ctccggtgag cggcggtgaa ccgaaagcca tcgacggtac
     2641 gctgtcagtg atggtgggcg gcgacaaggc tattttcgac aaatactatg atttgatgaa
     2701 agcgatggcg ggttccgtgg tgcataccgg ggaaatcggt gcaggtaacg tcactaaact
     2761 ggcaaatcag gtcattgtgg cgctgaatat tgccgcgatg tcagaagcgt taacgctggc
     2821 aactaaagcg ggcgttaacc cggacctggt ttatcaggca attcgtggtg ggctggcggg
     2881 cagtaccgtg ctggatgcca aagcgccgat ggtgatggac cgcaacttca agccgggctt
     2941 ccgtattgat ctgcatatta aggatctggc gaatgcgctg gatacttctc acggcgtcgg
     3001 cgcacaactg ccgctcacag ctgcggttat ggagatgatg caggcactgc gtgcagatgg
     3061 tttaggaacg gcggatcata gcgccctggc gtgctactac gaaaaactgg cgaaagtcga
     3121 agttactcgt taatgacgtc gtgcccggat ggcgtattgc aatccgggcc tggaatccag
     3181 gccgaataag agaaacgccc tccggcgtca tgtggtaaca ggcataggta tgaaaatcgt
     3241 aatcgcccca gactcttata aagaaagttt atctgccagc gaggttgcgc aggcgataga
     3301 aaaaggattt cgggaaattt ttcctgatgc acagtacgtt tctgttcctg ttgccgacgg
     3361 tggcgaagga acggtggaag cgatgattgc agccacccag ggttccgaac gtcacgcctg
     3421 ggttacaggg ccgctgggcg agaaagtgaa tgccagttgg gggatctccg gcgatggcaa
     3481 aaccgcgttt attgaaatgg cggcggccag tgggctggag ctggtacctg cggaaaaacg
     3541 cgatccactc gtgaccactt cacgcggcac aggcgagtta atcctgcagg cgctggagag
     3601 cggcgcgaca aacattatta tcggcattgg cggcagcgct acaaatgatg gcggcgcagg
     3661 catggtacag gcgctggggg cgaaattatg cgacgccaac ggcaatgaaa ttggttttgg
     3721 cggcggtagt cttaatactc tgaatgatat tgatatttcc ggcctcgatc cgcgcttaaa
     3781 agattgcgtc attcgcgtcg cttgtgatgt caccaatccg ctggtgggcg ataacggcgc
     3841 atcgcgcatc tttggcccac aaaagggagc cagtgaagcg atgattgttg agctggacaa
     3901 taacctctct cactatgccg aggtcattaa aaaagcgctg catgttgatg tgaaagatgt
     3961 ccccggcgca ggagctgctg gcggtatggg agcggcgctg atggcgtttc ttggtgcgga
     4021 actgaaaagt ggtattgaaa tcgtcactac ggcgctgaat ctggaggaac atattcacga
     4081 ttgtacgctg gtgatcaccg gtgaagggcg tattgacagc cagagtattc acggcaaggt
     4141 accgattggc gtcgcaaacg tggcgaagaa gtaccataaa ccggtgattg gcattgcggg
     4201 tagcctgacc gatgatgttg gcgttgtaca tcagcatggt attgatgcgg tctttagcgt
     4261 actgaccagc attggtacgt tggacgaagc attccgcggg gcttatgaca atatctgccg
     4321 tgcttcacgt aatatcgccg cgacactggc gattggaatg cgcaacgcgg ggtgacaagg
     4381 gcgcgcaaac cctctatact gcgcgccgaa gctgaccaga cagtcgccgc ttcgtcgtcg
     4441 tcctcttcgg gggagacggg cggaggggag gaaagtccgg gctccatagg gcagggtgcc
     4501 aggtaacgcc tgggggggaa acccacgacc agtgcaacag agagcaaacc gccgatggcc
     4561 cgcgcaagcg ggatcaggta agggtgaaag ggtgcggtaa gagcgcaccg cgcggctggt
     4621 aacagtccgt ggcacggtaa actccacccg gagcaaggcc aaataggggt tcataaggta
     4681 cggcccgtac tgaacccggg taggctgctt gagccagtga gcgattgctg gcctagatga
     4741 atgactgtcc acgacagaac ccggcttatc ggtcagtttc acctgattta cgtaaaaacc
     4801 cgcttcggcg ggtttttgct tttggagggg cagaaagatg aatgactgtc cacgacacta
     4861 tacccaaaag aaagcggctt atcggtcagt tttacctgat ttaacgaaaa cccgccagcg
     4921 taaactggcg ggtttttgct tttggtgcac agggtaagat gaataaatat ctacggtatt
     4981 aataatagac tttgtaagaa gttattaagt ctatattgca tttaaaaggt cgtaattata
     5041 cgtatagtcc tctctggtaa aatattggtt ctctgagtta tatgcttcat atgtgttata
     5101 ttccccgacc tcaaaaccat tgtccggaga attttcgtct ttctcgag
//
LOCUS       DAZFFG010000213         5140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFFG010000213 DAZFFG010000000
VERSION     DAZFFG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.214"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1883
                     /locus_tag="ACHJ7J_004818"
     CDS             <1..1883
                     /locus_tag="ACHJ7J_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5002919.1"
                     /translation="TIDEIRQKTSAMSLPEASDNEEKTRQALEEQNRLIDEQKSKIKS
                     LQEKIAGYQYVLANPGWTTDNGFMINHMTSVKTVTEGLAEATNQLAVEQSRLTQMQGK
                     AQSIQDVLAGLEERRVALIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQ
                     GLVNVPLRLPQATLDDKQQTALNNSERELALSRLKGEARERARLGYAADDLGFVGEAY
                     QTARLNYINNSLDAWRNNQANKPKAHKKTEAEKTEDIYKRLIKQQKEQIALAGQNTEL
                     AKMKYQVSQGELSTLSEAQKKTLLQNAALIDQKKIREQLAAYESSLADSNASARASDE
                     AQLLGYGEGSRMRERLQEMWSIRQAFEQKNNELLRQYQAGEIEEALWKQEKELNKKYL
                     EERLSDQQDYYAKADALRNNWNAGLQEGLTNWADSATDYASQAADAVVSTMDGLVSNI
                     SDALAGNVVDWRNWGSSVLREVSKILMNAAIVNGLKSLSGAGGWLGTVGGWISGAVAN
                     AKGGVYTSANLSAYSNTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAI
                     GNVNGGGGFVYSPVYHISIQNKGSNGEIDTQSARGLVDLIDSRVVSIMQSSRRDGGLY
                     SA"
     gene            1876..2217
                     /locus_tag="ACHJ7J_004819"
     CDS             1876..2217
                     /locus_tag="ACHJ7J_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5002920.1"
                     /translation="MPEPEVFNWIPREVMETTRKLSVITVKFGDGYEQRRAGGLNADL
                     KTFKPVFRVTDEYSRAALDSFLSRHAGMRAFLWRPPKHNRTVRVVCREWSISDNAMYT
                     DFNCTFEEVTH"
     gene            2217..2915
                     /locus_tag="ACHJ7J_004820"
     CDS             2217..2915
                     /locus_tag="ACHJ7J_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5002921.1"
                     /translation="MQDIQQETLNECTKTEQSALVVLWEIDLTEVGGDRYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSEADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNI
                     DNFGGFLSINKLSQ"
     gene            2920..3663
                     /locus_tag="ACHJ7J_004821"
     CDS             2920..3663
                     /locus_tag="ACHJ7J_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5002922.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAQMQGEVVALVHSHPGGLPWLSEVDRRLQVQSDLPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAEIEMPDFHREDDWWRNGQNLYLDNMEAT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFG"
     gene            3600..4202
                     /locus_tag="ACHJ7J_004822"
     CDS             3600..4202
                     /locus_tag="ACHJ7J_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5002923.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQIRIAGRDTGENELSARLNEPLENGAVIHIVPRLAGAKSGGIFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPTATSTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            4263..>5140
                     /locus_tag="ACHJ7J_004823"
     CDS             4263..>5140
                     /locus_tag="ACHJ7J_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5002924.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDPEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGA"
BASE COUNT         1288 a         1182 c         1575 g         1095 t
ORIGIN      
        1 caacaatcga cgaaattcgc cagaaaacgt cggcaatgtc gcttcctgaa gcgtcagata
       61 atgaggaaaa gacgcggcag gcactggagg agcagaacag gttaattgac gagcagaaaa
      121 gtaagattaa atccttacag gaaaaaattg ctggctatca gtatgtgctg gcaaacccgg
      181 gctggacaac cgataacggt tttatgatta accacatgac gtcggtaaaa acagtcacag
      241 aagggcttgc agaagcaaca aatcaactgg cagttgaaca gtcccgtctc acacaaatgc
      301 agggcaaagc gcaatccatt caggatgtgc ttgccgggct ggaggagcga cgagtggcgt
      361 tgatccgtca acaggcggcg gaacaaaaca aagcgtatca gtccctgttg atcatgaatg
      421 ggcagcatac cgagtttaat cgtcttcttg ggcttggtaa tgaattactt cagcagcgac
      481 aggggctggt gaatgtaccg ttacggctac cacaggcaac cctggatgat aaacagcaga
      541 ccgcactgaa taacagcgag cgcgaactgg ctctgtcccg cctgaagggg gaagcccgtg
      601 agcgtgcccg actgggttat gctgcggatg atctcggctt tgtaggagag gcgtatcaga
      661 cagcaagatt gaattatatc aataactcac tggatgcatg gcgaaataac caggcaaata
      721 aacccaaagc gcataaaaag accgaagcgg aaaaaacaga agatatttat aaacggctga
      781 ttaaacagca gaaagagcag atagcactgg cagggcagaa tactgaactg gctaagatga
      841 aatatcaggt cagtcagggc gaattatcaa ctctgtcaga agcgcagaaa aaaacgcttt
      901 tgcagaatgc agcactcatc gaccagaaaa agattcgtga gcagcttgct gcgtatgaga
      961 gcagtctggc ggacagtaat gccagtgccc gggcatctga cgaagcgcag ttgctgggat
     1021 atggtgaagg ctcacggatg cgtgaacgac tccaggaaat gtggagtatc cggcaggcgt
     1081 ttgagcagaa aaataacgag ctgctgagac agtatcaggc cggagaaatt gaagaagccc
     1141 tgtggaaaca ggagaaagaa ctgaataaaa aatatctgga agagcgtctc agcgatcagc
     1201 aggattatta tgcaaaggcc gatgctttac gtaataactg gaatgccgga ctccaggagg
     1261 gactgaccaa ctgggcagac agtgccaccg attatgcttc acaggcggca gatgctgtcg
     1321 tttccacgat ggacgggctg gtatcaaata tttccgatgc actggccggg aatgttgtgg
     1381 actggaggaa ctgggggagt tcagttctcc gggaagtttc aaaaattctg atgaatgcag
     1441 ccattgttaa cggactgaaa tcactctccg gtgccggagg gtggcttggt acggtcggcg
     1501 gatggatttc gggggcagtg gcaaacgcaa aaggtggtgt ttacacatcg gcaaatctga
     1561 gtgcttacag taacactatt gtggatacac cgacgtattt tgcttttgcg aaaggtgccg
     1621 ggctgatggg cgaggcaggg cctgaagcta tcatgccact gactcgggca gcggacggct
     1681 ctcttggagt cagagccatt ggcaatgtga atggtggcgg tggatttgtt tattctcccg
     1741 tgtatcacat cagtattcag aataaaggga gcaatggcga gatagatacg cagtcagcca
     1801 gggggctggt ggatctgatc gacagcaggg ttgtgtcaat tatgcagtca tcacgtcggg
     1861 atggaggatt atacagtgcc tgagcctgaa gtttttaact ggatcccccg tgaggtgatg
     1921 gagacgacac gaaagctatc agttattacg gtaaagtttg gtgacggata tgaacagcga
     1981 cgggctggtg gtctgaatgc ggatctgaaa acgtttaagc cggtatttcg tgtcacagat
     2041 gaatattccc gtgccgcgct ggacagtttt ttatcccgtc atgccgggat gcgtgctttt
     2101 ttgtggcgtc cgccaaaaca caacaggact gtccgggttg tctgcaggga gtggagcatt
     2161 tcggataatg ccatgtatac cgattttaac tgtacctttg aagaggtcac tcactgatgc
     2221 aggatataca gcaggaaaca ctcaatgagt gcactaaaac ggagcaatcc gcgctggtcg
     2281 tgctctggga aattgatctg acagaggtcg gcggagatcg ttatttcttc tgtaatgagc
     2341 agaacgaaaa aggtgaacca gtcacctggc aggggcggca gtatcaggcc tatcccattc
     2401 agggaagtgg atttgagatg aacggcaaag gagccagtgc aaggccaacg ctgaaagtct
     2461 ctaatctgca cggcatggtc accgggatgg cggaagacct gcagagtctg gtcggcggaa
     2521 cggtagtcag gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc gtcaacggaa
     2581 acagcgaagc tgatccggag caggaggtga tcagccgctg gcgcattgag cagtgcagcg
     2641 aactgagtgc ggtcagcgcc tcctttgtgt tgtccacacc gacggaaacg gatggtgccg
     2701 tttttccggg gcgtatcatg ctggccaaca cctgcacctg gacctatcgc ggtgatgagt
     2761 gcggttatag tggtccggct gtcgcggatg aatatgacca gccgacgtcc gatatcacga
     2821 aggataaatg cagcaaatgc ctgagtggct gtaagtttcg caataacatc gacaactttg
     2881 gcggcttcct ttccattaac aaactttcgc agtaaatcca tgacagaaac tgaatcagcg
     2941 attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gcggcttcgt ggttagaacg
     3001 ccggaggggg aaagatattt tccctgcgtg aatatctccg gtgagccgga ggcgtatttc
     3061 cggatgtcgc cggaggactg gctgcgtgca caaatgcagg gggaggttgt ggcactggtc
     3121 cacagccacc ccggtggtct gccctggctg agtgaggtcg acaggcggct gcaggtgcag
     3181 agtgatttgc cgtggtggct ggtctgccgg ggtgagattc acaagttccg ctgtgtgccg
     3241 catcttaccg ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat
     3301 gcataccatc tggcggaaat tgagatgccg gattttcacc gcgaggatga ctggtggcgt
     3361 aacggccaga atctctatct tgacaatatg gaggcgactg gtttttaccg tgtcgcactg
     3421 acagaggcgc agccgggcga tgtgctgctg tgctgttttg gttcatcggt gccgaatcat
     3481 gccgccattt actgtggtga cggcgagctg ctgcaccata ttcctgaaca actgagcaaa
     3541 cgagagaggt ataccgacaa atggcagcga cgcacacact cactctggcg tcaccgggca
     3601 tggcgcgcat ctgcctttac ggggatttac aacgatttgg ccgccgcatc gaccttcggg
     3661 tgaaaacggg agccgaagcc atccgggcac tggccacaca gctcccggcg tttcgtcaga
     3721 aactgagcga cggctggtat cagatacgga ttgccgggcg tgatacaggt gaaaacgaat
     3781 tatcagcccg tcttaatgag ccgctggaaa atggtgccgt gatccacatc gtgccacgtc
     3841 tggcgggtgc taaaagtggc ggtatttttc aggcagtgct gggggcggct gttatggcag
     3901 ttgctatatg gatgccagga gtaggaatta tggcgagtaa tctgctgttt tctctcggtg
     3961 ccagtatgac tctgggtggt gtggcccaga tgctggcacc gaaagcccgg acgcccacag
     4021 cgaccagcac ggataacggt aagcagaaca cctatttctc atcactggat aacatggttg
     4081 cccagggcaa tgttctgcct gttctgtacg gtgaaatgcg tgtggggtct cgtgtggttt
     4141 ctcaggagat cagcacggca gatgaagggg acggtggtca ggttgtggtg attggccgct
     4201 gatgcaaaat atttcatgtg aaaccgcctc cgggcggttt tgtcgtttat ggagcgtgag
     4261 gaatgggtaa aggcagcagt aaggggcata ccccgcgcga agcgaaggac aacctgaagt
     4321 ccacgcagtt gctgagtgtg attgatgcca tcagtgaagg gccggttgaa ggtccggtgg
     4381 atggattaaa aagcgtgctg ctgaacagta cgccggtgct ggacagtgag gggaatacca
     4441 acatcgccgg tgtcacggtg gtgttccggg ctggtgagca ggagcagaca ccgccggagg
     4501 gatttgaatc ctccggatcc gagacggtgc tgggtacgga agtgaaatat gacacgccga
     4561 tcacccgcac catcacgtcg gcaaacattg accgtctgcg ttttaccttc ggtgtgcagg
     4621 cactggtgga aaccacctca aagggtgacc ggaatccgtc ggaagtccgc ctgctggttc
     4681 agatacagcg taacggtggc tgggtgacgg aaaaagacat caccattaag ggcaaaacca
     4741 cctcacagta tctggcctcg gtggtggtgg gtaacctgcc gccgcgcccg ttcagtatcc
     4801 ggatgcgcag gatgacgccg gacagcacca cagaccagct gcagaacaaa acgctctggt
     4861 cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggca ctggtcggcg
     4921 tgcaggtgga cccggagcag ttcggcagcc agcaggtgag ccgtaattat catctgcgcg
     4981 ggcgtattct gcaggtgccg tcgaattata acccgcagac gcggcaatac agcggtatct
     5041 gggacggaac gtttaagcca gcatacagca acaacatggc ctggtgtctg tgggatatgc
     5101 tgacccatcc gcgctacggc atggggaaac gtcttggtgc
//
LOCUS       DAZFFG010000214         4909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFFG010000214 DAZFFG010000000
VERSION     DAZFFG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.215"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            315..1190
                     /locus_tag="ACHJ7J_004824"
     CDS             315..1190
                     /locus_tag="ACHJ7J_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5002925.1"
                     /translation="MTKPHGLKALEQPLSALPDTLRQLIFERIQNLTHYEPMIGIMGK
                     TGVGKSSLCNELFRGEVSPVSDVNACTREVLRFRLRSGRHSLVIVDLPGVGENGQQDH
                     EYRALYRRMLPGLDLVLWVIKADDRALSVDEQFWHGVMQPYQHQVLFVLNQADKIEPC
                     HEWDIRTATPSPQQQTNLKAKQAVITTMFTPRHPVCVVSALFDWGIEEMVSTMMRCLP
                     DRATSPVATQLHGRLCTEQVRSQARHGFGEAVGQVMDRVELSSFLPTTLKVVLRTVRD
                     VVVSVARTIWDWIFF"
     gene            1405..2112
                     /locus_tag="ACHJ7J_004825"
     CDS             1405..2112
                     /locus_tag="ACHJ7J_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002926.1"
                     /translation="MTQAERRHDRLAVRLSIIISRLVAGETLDIRKLAAEFDVSVRTL
                     RRDFRERLMYLDLEYRQGQYRLLSGSRQRELAVMTFARKSGVKSLFPGLDGHLVSSLL
                     GGAGESPCLIWQGDSAQPVTDSEVFIRLVNAVQECRKVTLRGNGCNHTDISPYRLVLH
                     AGSWYLTGARQTRIIALPLADIRGVTLHPDIFTPDTTVRDILSRPDFLQALPHFRFIG
                     EVLSGFVPEPHQPATEG"
     gene            2116..2262
                     /locus_tag="ACHJ7J_004826"
     CDS             2116..2262
                     /locus_tag="ACHJ7J_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002927.1"
                     /translation="MKIRRIIPGNPTSGKRCPDCGSYDTIPWSDGWMCRDCGCEWGKD
                     NGYL"
     gene            2275..2490
                     /locus_tag="ACHJ7J_004827"
     CDS             2275..2490
                     /locus_tag="ACHJ7J_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5002928.1"
                     /translation="MGFWDTAGKLAKEVAKDALYSAQEVRAIHDRLESRSSSELRRII
                     SDNVMFSSATETEKRLARKVLRGRAEL"
     gene            2596..3006
                     /locus_tag="ACHJ7J_004828"
     CDS             2596..3006
                     /locus_tag="ACHJ7J_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002929.1"
                     /translation="MKAFIFPLMVSGLLLSASVNATEVKLPRTIIGVKDGSELMCGSE
                     DVKDGICSLIGVSDDGKYALLSSPGGSACYDPYIIVVNLATSKGKMMDYNRHATCSGE
                     ATAKFVRSSDKDHLTVVIYDPSIKKNVGLNTIDF"
     gene            3072..4010
                     /locus_tag="ACHJ7J_004829"
     CDS             3072..4010
                     /locus_tag="ACHJ7J_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002930.1"
                     /translation="MSLVLRTFNQVMLISSLCLISAGVYAAGVAKTAADAMRCDPDAG
                     DYENGYGSCPLLGNIYDADPVFKKDLDDALKSAGLSGLTGRQGAMNGPDSGLIPVDVG
                     GEKWLLGAVCERGNCGDHYLKILYIPSEHVIAGFYYNSGEENMFGDVGDAEAQILRRD
                     VPNATSVQQPPAMPVTPANDAPANTVWTFKGDSGKTLWQVCGGQSSTCAIIANTKYYV
                     AILNRKSATGCAFGDFYVAARDTATWQQYDTGTCSADAYIRKGSINNGQYLSVDIGIN
                     GVMVKQFPIGYWSMQKEFSGNRRPSWSKVKEKNQQH"
     gene            4100..>4909
                     /locus_tag="ACHJ7J_004830"
     CDS             4100..>4909
                     /locus_tag="ACHJ7J_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5002931.1"
                     /translation="MRLASRFGRINQIRRDRPLTQEELMQVVPSVFGEDKHTSRSENY
                     AYIPTIIVLESLQREGFQPFFACQTRVRDSGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLALPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLES"
BASE COUNT         1176 a         1216 c         1345 g         1172 t
ORIGIN      
        1 tgaatgtatg tgatgacgca tgccgtaatg atgaacaact gatatcagta atttgaagac
       61 gagaatatcg tttatccctg cgttgaagtt ctatggagcg cttcttcagc agcctgaaaa
      121 cagaatgagt gccagcgaat gattacgcaa gcagggacga ggtccggcag tgaattaatc
      181 gttgcatttt gagttgatac aaattggcca catgtacctg tattccaaat aatttcagaa
      241 agatattatc tccgtgaatc cccgcctcac tgaccggagt gcgggtttta tgttttacac
      301 gcagagacct ctttatgact aaacctcacg gccttaaggc ccttgaacaa ccgctgtcgg
      361 cgcttcccga tacgcttcgc cagctaattt ttgaacgtat tcagaacctc acccactacg
      421 agccgatgat aggcatcatg ggcaagactg gggtcggcaa atcctcgctc tgtaatgaac
      481 tattccgggg ggaagtctcc cccgtcagtg atgtgaacgc ctgtacccgt gaggtgctgc
      541 gcttccggct gcgcagcggg cgtcacagtc tggtgattgt tgatttgccg ggcgtgggcg
      601 aaaacgggca acaggaccat gaataccgtg ccctgtaccg gcgtatgctg ccggggctgg
      661 acctggtgct gtgggtcatt aaagccgacg accgggcgct gtcagtggac gagcagttct
      721 ggcatggggt gatgcaacct taccagcacc aggtgctgtt tgtgctgaac caggccgaca
      781 aaatcgaacc ctgccacgaa tgggatatcc gtaccgctac cccctcgcca cagcagcaga
      841 ctaacctgaa ggcgaagcag gccgtcatta ccacgatgtt cactccccgt catccagtct
      901 gcgtggtgtc agcgttgttc gactggggca ttgaagagat ggtgtcaact atgatgcgtt
      961 gtctgccgga cagggccacc agtccggtgg cgacgcagtt acatgggagg ctctgtaccg
     1021 aacaggtccg gtcgcaggcc cgtcatggct ttggtgaagc ggtggggcag gtcatggatc
     1081 gggtagaatt atcgtcgttc ctgccgacaa cactgaaagt ggtgctacgt accgtgcgtg
     1141 atgtagtggt gtcagttgcg cggaccatct gggactggat tttcttctga tactgagaag
     1201 gtattttgct aacgtgcgga cgcatcctgt ccgtttgcag acctataatc agagagaaaa
     1261 gagctacgtc cctccgtggc ccccgccatt cctgacactg agcattttta aaaatcgtct
     1321 ttctgtgtcc ataccctgcc tctcgtcctc gttaatatta ccctcatctt ttcattgaat
     1381 tagcttaccg gagaacgaag ccttatgact caggctgagc gtcgccatga ccggcttgcc
     1441 gtcagactgt cgataattat aagtcgtctg gtggcaggtg aaacgctgga tatacgcaaa
     1501 ctggcagctg agtttgatgt gtcagttcgc accctgcgtc gggactttcg tgaacgactg
     1561 atgtacctgg acctggagta tcgacagggc cagtaccgtc tgctgtccgg cagccgtcag
     1621 cgagagctgg cggtgatgac ttttgcccgt aagtcgggcg ttaagtccct gtttccggga
     1681 cttgacggac atctggtcag ctcacttttg gggggggcgg gtgaatcccc ctgccttatc
     1741 tggcaggggg attctgcaca gcccgtcacc gacagtgagg tctttatccg gctggtaaat
     1801 gctgtccagg aatgccggaa agtgacactg cgcggcaacg gatgcaacca tacggatatc
     1861 tcaccctacc gcctggttct tcatgccggt agttggtatc tgacaggggc acgtcagaca
     1921 cgcatcatcg cattgccact ggctgatatc cggggcgtca cacttcatcc cgacattttt
     1981 acccccgaca ccaccgtgcg cgacatcttg tcccgtccgg attttttaca ggcattacct
     2041 catttccggt tcattggcga agtgctttcc ggcttcgtcc ctgaaccaca tcaacccgca
     2101 acagaaggat aacatatgaa aatcagacgt atcatccccg gtaacccgac ttccggcaaa
     2161 cgctgcccgg actgtggctc ttacgacact attccttggt ccgatggctg gatgtgcagg
     2221 gactgcgggt gtgagtgggg caaagataac ggttacctgt aaaggggaaa taagatggga
     2281 ttctgggata ccgcaggaaa actggctaaa gaagttgcaa aagacgctct gtacagtgcc
     2341 caggaggtaa gagcgattca tgaccggctg gagagcagga gtagctcaga gctgagaagg
     2401 ataatcagcg acaacgttat gttcagcagc gcaacggaaa cggagaagcg gctggcacgc
     2461 aaggttttac gtggccgggc cgagctctga ttatgccaca gggtctgtgc aggccctgtc
     2521 tggcgtttag atacagggcg gcagcctttc caccacacaa tcattttcat tcaaaaaaat
     2581 aaacaggatg aatatatgaa agcattcata tttccattaa tggtttcagg tttgttgttg
     2641 agtgcatcag ttaatgccac agaggttaaa ttaccccgta ctataattgg tgtgaaagat
     2701 ggaagtgagc tgatgtgcgg aagtgaggat gtaaaagacg ggatatgcag cctcattggt
     2761 gtctctgatg atggcaaata tgccttgctg agcagtccgg gaggttcggc ctgttatgat
     2821 ccttatatca tcgttgtcaa tcttgctacc agcaaaggta aaatgatgga ttataaccgt
     2881 catgcaacat gcagtggtga ggccaccgct aagtttgtcc gttcttccga caaagaccat
     2941 cttaccgttg ttatttacga cccctccatc aaaaagaatg tcggtcttaa taccattgac
     3001 ttttaaatct tttacagggc tcttaaccga gcccgtttct tttatataaa caggatggaa
     3061 taaaagtaat catgtctctt gttttacgta catttaatca ggtaatgctg atatcgtcgc
     3121 tttgtttgat ttccgccgga gtttacgccg ccggtgttgc aaaaacggca gcagatgcaa
     3181 tgcgctgtga tcctgacgca ggtgattatg aaaacggata cggttcttgc cctcttctgg
     3241 gcaacattta cgatgcggat ccggtattta agaaagacct tgatgatgcg ctaaagtcag
     3301 caggactttc cgggttaaca gggaggcagg gtgcaatgaa cggcccggac agtggtttaa
     3361 taccggtgga tgtaggtggc gagaagtggc tgttgggggc tgtctgtgag cggggtaatt
     3421 gcggtgacca ttatcttaaa atactgtata tcccctcaga gcatgttatt gccggttttt
     3481 attacaacag cggagaggaa aacatgtttg gcgatgtcgg ggatgcagaa gcacaaatat
     3541 tacgccgcga tgtacccaac gctacatcag tgcagcagcc accggcaatg cctgtaacac
     3601 ctgcaaacga cgcaccggca aatactgtct ggacctttaa gggggacagt ggcaaaacgt
     3661 tatggcaggt ctgcggcggc cagagcagca cttgcgccat tatcgccaac actaagtact
     3721 acgttgccat actgaaccgc aaatcagcga cgggctgtgc tttcggtgat ttttatgttg
     3781 ctgcccgcga taccgcaaca tggcagcagt acgataccgg tacctgcagc gcggatgcgt
     3841 atatccgcaa gggcagcatc aacaacggcc agtatctgtc cgtcgatatt ggtatcaacg
     3901 gggtgatggt gaagcagttc ccgattggtt actggtcaat gcagaaggaa ttttccggta
     3961 accgacgccc ctcatggtca aaagttaaag agaaaaatca gcaacattaa ttatcagcta
     4021 taacatattc catcaggcca tgttcctccg gggacatggc ctttttgctt ttatttcatt
     4081 atcagataag gagttattta tgcgattagc cagtcgcttc ggacggatta atcagatacg
     4141 ccgggaccgc ccactgaccc aagaagaatt aatgcaggtc gtccccagcg tattcgggga
     4201 agacaaacac acctccagaa gcgaaaacta cgcgtacatc cccaccatca tcgtgctgga
     4261 aagcctgcag cgggaaggtt ttcagccctt tttcgcctgc cagacccgag tgcgcgactc
     4321 gggccgccgg gggtatacca aacacatgct gcgtctgcgc cgtgacgggg agataaacgg
     4381 acagcatgtc cctgaaatca tcctgctgaa ctcccatgac ggtacctcca gctaccagat
     4441 gctgccgggt tacttcagat ttgtgtgtca gaacgggtgc gtctgtggtc agtctctggg
     4501 ggaagtgcgt gttccgcacc ggggaaatgt ggtggacaga gtcattgaag gggcttacga
     4561 ggtggtgggc gtgtttgacc ggatagagga gaagcgtgat gccatgcagt cgctggccct
     4621 gccgccaccg gcacgccagg cgctggcaca ggcggcactg acttaccgtt atggtgacga
     4681 acatcagccc gtcaccaccg ccgacattct gacgccacga cgcagggagg attacggtaa
     4741 ggacctgtgg agtgcttatc agaccatcca ggagaatatg ctgaaaggcg ggatttccgg
     4801 ccgcagtgca aaaggaaaac gtatccacac ccgtgccatt cacagcatcg acaccgatat
     4861 taagctcaac cgggcgttgt gggtgatggc agaaacgctg ctggagagc
//
LOCUS       DAZFFG010000215         4850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFFG010000215 DAZFFG010000000
VERSION     DAZFFG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.216"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..362)
                     /locus_tag="ACHJ7J_004831"
     CDS             complement(<1..362)
                     /locus_tag="ACHJ7J_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="icd-like protein"
                     /protein_id="HFO5002932.1"
                     /translation="MVYKTKATGGGRQWHNTKLEHIQNTRLSAGGQCYQSGSICCHAC
                     NEHIFLEYSLRYFSRTNAHGANLSGSCSFTAFLRCFFLDEDNFFNNLSVIVDTCNPVR
                     HSSPHHGAGDGYSCSLALR"
     gene            complement(371..592)
                     /locus_tag="ACHJ7J_004832"
     CDS             complement(371..592)
                     /locus_tag="ACHJ7J_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002933.1"
                     /translation="MSKVKSSQDQFDRIIREEECRRLTGICRTTRYMMERKGVFPARR
                     QLGARSVGWLLSEVNDWLDRQPKAQLKSA"
     gene            complement(682..1419)
                     /locus_tag="ACHJ7J_004833"
     CDS             complement(682..1419)
                     /locus_tag="ACHJ7J_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002934.1"
                     /translation="MKKISIPERIYYPLPEAAKKLGCTLNDIYHFAAIGALNISVYFP
                     NCHPCKSLVIPKSLVENIDVDSGGMLQGDRWVITGMKPVGSDFEWVNYFGVASYLAKN
                     FCGFFYLNRESFSELEFIGNDARIVSSFFSTRPESENWECQIMSDGAIEIDRRFLCVM
                     AKDLEHIKDVGTMPGRKMGESPKTLAKKAELIPALIKLIPEMDDIDIGTAPVAKVIAV
                     LEAAAAMKGVFLPATDKNTWAKYLGRT"
     gene            complement(1416..2639)
                     /locus_tag="ACHJ7J_004834"
     CDS             complement(1416..2639)
                     /locus_tag="ACHJ7J_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5002935.1"
                     /translation="MAGGTNKLSDTSLRKMLGREIDRDRFYADGDGLSIKVSKIGVLT
                     WYFSFRIGGRESYSQRVKLGNYPDLSLKAAREKREQCRAWLAEGKNPKHRLNVAIHET
                     LKPVTVKEALDYWIREYAVYKRTNVDKHIEQLRKHIFPYIGDYPLSMCETRHWLECFA
                     RVRNDAPVAAGYLLQMCKQALKFCRVHRYAVSNALDDLTIDDVGRKQNKRDREHSSQE
                     LADIWQECSGKKFKPYYSSLLRLLVVFGCRTQELRLSRITEWDLKDWIWTVPKEHSKG
                     GEKILRPIPVVIRPFIQQLIEQHKNSGLLLGEIKKPEAVSQWGRMIYKRLGHIESWTL
                     HDLRRTFATTLNNMGIAPHVVEQLLGHTLGGVMAIYNRSQYLPEKLDALNKWMERLEV
                     ISGQYSNVKILKVAK"
     gene            2917..3801
                     /gene="yhdJ"
                     /locus_tag="ACHJ7J_004835"
     CDS             2917..3801
                     /gene="yhdJ"
                     /locus_tag="ACHJ7J_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5002936.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            3885..4064
                     /locus_tag="ACHJ7J_004836"
     CDS             3885..4064
                     /locus_tag="ACHJ7J_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO5002937.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(4067..4729)
                     /gene="acrS"
                     /locus_tag="ACHJ7J_004837"
     CDS             complement(4067..4729)
                     /gene="acrS"
                     /locus_tag="ACHJ7J_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO5002938.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFARHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         1399 a         1139 c          949 g         1363 t
ORIGIN      
        1 cgcaaggcca aagagcagga gtaaccatca ccagcgccgt gatgcggtga actgtggcgc
       61 acagggttac aggtatctac gatgactgac aaattattaa agaaattatc ctcatctagg
      121 aaaaaacaac gcaaaaatgc ggtaaacgaa catgaaccgg agagattcgc accatgtgcg
      181 tttgtccttg agaagtacct taaagagtat tccaggaaaa tatgctcatt gcaggcatgg
      241 caacaaatcg atccagactg atagcattgc ccaccagccg acaacctggt attctggata
      301 tgctcaagtt tagtgttatg ccactggcga ccgcccccgg tcgcctttgt tttatatacc
      361 ataactcacc ttacgccgat ttcagttgtg ctttgggttg acgatcaagc caatcattaa
      421 cctcagacag caaccaaccg acagaacgag cccccaactg acgacgggct ggaaacaccc
      481 ctttcctttc catcatataa cgcgtcgttc ggcaaatacc ggtaaggcgt cggcattcct
      541 cctcacgaat gatgcgatca aattggtctt gcgatgattt aactttgctc atatggatat
      601 gccctcgttt attatcgttc gagagcatat tgataatatg cttagaatat ttaggtgata
      661 tcacatattt tatatgtgat atcacgttcg ccctaagtat ttagcccacg tattcttatc
      721 tgttgcggga agaaacaccc ctttcatcgc tgccgcagcc tctaaaacag cgatcacttt
      781 agcaactgga gcagttccaa tatctatatc atccatttct ggaattagtt tgattaatgc
      841 agggatcaac tcagctttct tcgcaagagt ttttggcgat tctcccattt ttcttccagg
      901 catggttcca acatctttga tatgctccaa atccttagcc attacacata aaaaccttct
      961 gtcgatctca atagccccat cactcataat ttgacattcc cagttttctg attcaggacg
     1021 agttgaaaaa aaagatgata ctatccttgc atcattgcca ataaattcaa gttcagaaaa
     1081 actttcccga tttagataga agaaaccgca aaaattcttt gcaagataag aagccacacc
     1141 aaaataattt acccattcaa aatcgctacc aacaggcttc atccctgtaa ttacccagcg
     1201 atccccttgc aacatccctc cagaatcaac atcaatattt tcaactaatg attttggtat
     1261 aactagcgat ttacatggat gacagtttgg aaaataaaca gaaatattca aagcacctat
     1321 agcagcaaaa tgatatatat cattaagcgt acatcccagc ttctttgctg cttcaggcaa
     1381 tggataatat atacgctcag ggatgcttat ctttttcatt tcgctacctt caatattttt
     1441 acatttgaat actgaccaga aataacctca agccgttcca tccatttatt tagagcgtct
     1501 agtttttctg gtaagtactg gctacgattg tatatggcca ttacaccacc taatgtgtga
     1561 ccgagaagct gttcaacaac atgtggtgca atccccatgt tatttagtgt ggtagcaaac
     1621 gtcctgcgca ggtcgtgaag tgtccacgat tcaatatgcc ccagacgctt atatatcatc
     1681 ctcccccatt ggctgacagc ttctggtttc tttatttcac ccaacaaaag acctgagttt
     1741 ttgtgttgct ctataagctg ttggatgaat gggcgtatta ccactggaat agggcgcaat
     1801 atcttctctc cgcctttgct gtgttcttta ggcactgtcc agatccaatc ctttaaatcc
     1861 cactctgtaa tacgtgacaa ccgaagctcc tgggttcgac atccaaacac caccaataga
     1921 cgcaacaacg atgaatagta tggtttaaat ttcttacctg aacattcctg ccaaatatct
     1981 gctaactctt gactgctgtg ttccctatcg cgcttattct gttttctacc aacatcatcg
     2041 atagtcaggt catccagagc attgctaacc gcataacgat gtacgcggca aaacttcaga
     2101 gcctgcttac acatctgcag caaataacca gctgccactg gagcgtcatt ccttactcta
     2161 gcgaaacact ccagccaatg ccgggtttcg cacattgata acggataatc acctatatag
     2221 gggaaaatat gcttcctcag ttgctcaatg tgtttatcaa cattcgtgcg cttgtaaacg
     2281 gcgtattctc tgatccagta atccagagcc tcttttaccg tcactggttt gagtgtttca
     2341 tgtattgcca catttaaccg atgctttgga tttttacctt cagcaagcca tgctcgacac
     2401 tgttcacgtt tctcgcgcgc agctttcaga gaaagatccg ggtagtttcc aagcttaaca
     2461 cgctgtgaat acgattcacg cccaccaatc cggaaagaga aataccaagt aagaacgcca
     2521 atttttgaca ccttgatact caatccatca ccgtcagcat aaaatctatc tctatcgatt
     2581 tccctcccca gcatcttacg caacgatgta tcgcttaatt tgttcgttcc tccagccata
     2641 gaaaacctta attcatattg aaaactgact gcaccactga ctgcacactt taatattacc
     2701 ctatgaacag aaatgaacaa aggtaaacta aagaaacaac attaatcatt ttattcaata
     2761 agttatataa acactaacga accatgacaa actgtaagaa acataggttt tgataatacg
     2821 gcatgaacta attcaggtta gctaaatgct tgattaaaaa ggcgctactc ggcatgggga
     2881 agcgcctttt ttataggtgt cacaaaggga gtgaccatga gaacaggatg tgaaccgacc
     2941 cggtttggta atgaagctaa gaccattatt cacggtgatg cccttgccga acttaaaaag
     3001 ctacctactg aaagcgtcga tctgatcttt gccgacccac cgtataacat cggtaaaaat
     3061 tttgatggtc tgatcgaagc ctggaaagaa gatctgttta tcgactggct gtttgaagtg
     3121 attgcagagt gccaccgcgt tctgaaaaag cagggcagca tgtacatcat gaacagtacg
     3181 gaaaacatgc cctttatcga tctccagtgc cgcaagcttt ttaccatcaa aagtcgcatc
     3241 gtctggtcat atgacagttc tggagtgcag gcgaaaaaac actacggttc catgtatgaa
     3301 cccatcctga tgatggtgaa agacgcaaag aactacacgt tcaacggtga tgctattctg
     3361 gtagaagcca aaaccggatc gcagcgcgcg ttgatcgatt atcgcaaaaa tcctccacag
     3421 ccttacaatc atcaaaaagt accgggtaac gtctgggatt ttccgcgcgt gcgttatttg
     3481 atggatgaat atgaaaacca cccgacgcaa aaaccggaag ccttactgaa acgcattatt
     3541 ctcgcctctt ccaacccagg cgatatcgtt ctcgacccgt ttgccggtag ctttactacc
     3601 ggtgccgtag ccgtctccag cgggcgaaaa ttcattggta ttgagatcaa cagcgagtac
     3661 atcaaaatgg ggcttcgacg gctggatgtc gcgtcgcatt actctgcgga agaactggcg
     3721 aaagtgaaaa aaagaaagac cggcaacctg tcaaaacgaa gtcggttaag cgaagttgac
     3781 cccgatctca ttgcaaagta aaggaagtgt aagcctggtt ttcagattat tcatttcgtg
     3841 tatatttcct gccagacttg gttaaacatg cacaggctca ggtaatgatt cgcaagtatt
     3901 ggtggctcgt cgttttcgct gtcttcgttt ttctgtttga tactttactg atgcagtgga
     3961 ttgaactgct ggcaacagaa acagacaaat gccgcaatat gaactcagtt aatccactaa
     4021 aactggttaa ctgtgacgaa ctgaattttc aggacagaat gtgaatttac atgacactta
     4081 attcattcgt ttgatgaatt aatttcgtta tgttttcatc tggcatgaac attcttaata
     4141 cgttatcgac cagagcgggg gcttgtttat aaagatcata acccgccata ttcattaacc
     4201 agttttgaac aattccgctg aaggcaccat caataataat catcacaaca tctaaatcga
     4261 ggttatttgc tacacaacct tgttgctgac acgcctgcaa tacttcgcgg agagtctgcg
     4321 gattaaagcc catcttttcg cgtatcactc cctcggccag catctcatca ttaaattcac
     4381 atttgtgata taagattttc agcaacgcct gctggcgggg aattttggca atatattgca
     4441 agccgacaat caatttttca cgcaattgtt gaaacgggtc atgctctaat ccagccgtca
     4501 agtgttcctg gattaactcc cgcaatgaag gctgttgcaa ccacatctca ttaaacagtt
     4561 gagtcttgtt ttcgaagtgc cagtagatag cgccacgcgt aacgttagcg gcgtcggcaa
     4621 tgtcgttgag cgtcgtcttg cttacgccat gccgcgcaaa ctgggcgatg gcagtttcaa
     4681 tcagttcttg ccgggtcttc agagcttcgg ctttggttct ttttgccatg attaattatt
     4741 caggaaataa atatattcga cacagggtga gaaaatagcg aaggttaatc tatcacctaa
     4801 tgtgtattta tacgagaggg taatattgag ttgctataaa tcgtcaaata
//
LOCUS       DAZFFG010000216         4805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFFG010000216 DAZFFG010000000
VERSION     DAZFFG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.217"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            97..1383
                     /locus_tag="ACHJ7J_004838"
     CDS             97..1383
                     /locus_tag="ACHJ7J_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5002939.1"
                     /translation="MSRNTLNKLTDRQCKAAKPRDKTYKLSDGGGLYLEVSPTGSKYW
                     RMKYRRPSDKKEDRLAFGVYPTISLQDAREKRDNTKKLLVKGIDPKAEQRAAKAEEKG
                     AFTFETIGRQWVESHQMWNEAHRKRVLRSLEMYIFPHIGNSDIRKLEAMTILPLFKKV
                     DDAGKHDTANRLKQRVKDIIHSARLSGIKTEIITNDLDVRLMQYETKHHAALHPRDLP
                     DFFTRLGSFRGNPLTRLAIELTMLTFVRSSELRFARWKEFDLDRAEWIIPKKREPIDG
                     VRFSTRGTKTGKDEHIVLLSRQAVSIVKQLRELSGSYDVVFPNERNTKGVMSENTVNK
                     ALRLMGYNTQEDVTGHGFRATACSSLMESGLWQEDAVERQMSHKEYKDVKRAYKHKAD
                     YLEERKLMLQWWADYLDANREIHISPYEFGRRTRRD"
     gene            1385..2116
                     /locus_tag="ACHJ7J_004839"
     CDS             1385..2116
                     /locus_tag="ACHJ7J_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002940.1"
                     /translation="MTGLDAFYNESEDKIWFGNLISELARLNNENERVIAAAILSNYR
                     LSKTKPDTLGGLGFYQFDWVSGFTTNEDFEKQCVEFLGLLAMGHEYRESPIPGEEGTF
                     NVRKGEDFLYHDDEDDWYGFYDSFYFKRSELVSFFPELEDHNIDEPIAEETESGSLPS
                     DWRGKNTAWKMIAGLAIALYESSEDVRKNGKLNQSAVCEKAANNIAKYSEEYNDAGMT
                     MDNLRTLLKKTLQRHAPNFLGKITK"
     gene            2274..2465
                     /locus_tag="ACHJ7J_004840"
     CDS             2274..2465
                     /locus_tag="ACHJ7J_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5002941.1"
                     /translation="MKKSLIRLSEVLKRTGYSKAWVYALMSRGQFPQSVKIGARAIAF
                     IESEIDDWIDQRIAESRSN"
     gene            2514..2705
                     /locus_tag="ACHJ7J_004841"
     CDS             2514..2705
                     /locus_tag="ACHJ7J_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002942.1"
                     /translation="MQLTPTNQKLNVTHVDFETFAELIRSDPQKVSTSTWVYDVKSTN
                     GGKAIGIVSGDDYLVIDLI"
     gene            2788..2982
                     /locus_tag="ACHJ7J_004842"
     CDS             2788..2982
                     /locus_tag="ACHJ7J_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5002943.1"
                     /translation="MINYLTAKDAAEYLGVCLSRFYQLKRYIPSFPPYVNQTINGRKR
                     RVWLASSLDQFADRFLGGRK"
     gene            2979..3365
                     /locus_tag="ACHJ7J_004843"
     CDS             2979..3365
                     /locus_tag="ACHJ7J_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002944.1"
                     /translation="MSTLTRTQVAANIRDSLLSGRKLTPKEFDEILRKAGNHERSRVL
                     TLLRNDWGIPVEQFKTGAYHVTERNLEAYHSDKDETLKIWRTNARYVKTLRKVNITLS
                     LLRGLVGKVPEDTLRTIYKGIETKYL"
     gene            3839..4486
                     /locus_tag="ACHJ7J_004844"
     CDS             3839..4486
                     /locus_tag="ACHJ7J_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5002945.1"
                     /translation="MMNERSINGQTGKIISKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGRQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRKARVKASRARRESGGYDLYY"
BASE COUNT         1603 a          967 c         1127 g         1108 t
ORIGIN      
        1 tattctcatg acgtccattg ccgtctattg aaatcaagta aatctatggg gcataattgg
       61 gggcattgct ggttcgattg tatatgtgcc cccaatatgt ccagaaatac cctcaacaaa
      121 ttaacagacc gtcagtgcaa agcagctaag ccacgagaca aaacttataa attgtctgat
      181 ggtggtggtc tttatcttga agtatccccg accgggtcga agtattggcg aatgaagtat
      241 cgccgcccat cagataagaa agaagacaga cttgcttttg gtgtatatcc aaccattagc
      301 ttacaagacg ccagagaaaa acgagacaac accaaaaagt tgttagtcaa ggggatagat
      361 cccaaagcag agcaaagagc agcaaaagca gaagaaaaag gtgcattcac ttttgagaca
      421 ataggacgtc aatgggttga aagccaccag atgtggaacg aagcccacag aaaacgcgta
      481 cttagaagcc ttgaaatgta tatttttcct cacattggca actcggatat tcgcaaactg
      541 gaggcgatga caattttacc tttgtttaag aaggtagatg acgcagggaa acacgataca
      601 gctaaccgac ttaaacaacg agtaaaggac atcatacata gtgcccgtct gagcggcatt
      661 aagacagaaa ttatcacgaa tgatctcgat gtccggttaa tgcaatatga aactaagcat
      721 catgccgcat tacatcccag agacctacca gactttttca ctcgattggg aagttttaga
      781 ggtaatcctc ttacccgcct tgccattgag ctaaccatgt tgacctttgt tcgttcaagc
      841 gagttaaggt ttgcccgttg gaaagagttt gaccttgatc gggctgagtg gattatccct
      901 aaaaaaagag agcctatcga tggtgtgagg ttttccaccc gtggcacaaa gacgggaaaa
      961 gacgaacata tcgtgcttct tagccgtcag gctgtttcta ttgtaaagca gttgagagaa
     1021 ctaagcggaa gctacgatgt tgtgtttccg aatgaaagaa acaccaaagg cgtgatgagt
     1081 gaaaacacag taaacaaggc attacgttta atgggatata acacgcaaga ggacgtaact
     1141 ggacatggat tccgtgccac agcttgtagt tccttgatgg agtccggttt gtggcaggaa
     1201 gacgcagtag agcgccagat gagccacaaa gaatataaag acgttaaaag agcctacaag
     1261 cacaaggctg attacttaga agagcgcaaa ctaatgcttc aatggtgggc agattatctg
     1321 gacgccaatc gagaaataca tataagccct tacgagttcg gaaggagaac acgccgtgac
     1381 taagatgaca ggactagatg cgttttataa cgagtcagaa gataaaatct ggtttggtaa
     1441 tctgatcagc gaacttgcaa gactgaacaa tgaaaatgaa agagtgatag ctgccgctat
     1501 cttaagtaac tacagattga gcaagactaa accagatact ttaggggggc ttggctttta
     1561 tcagttcgat tgggtatctg gttttacgac taatgaagac tttgaaaagc agtgtgtgga
     1621 gtttttaggt cttctggcta tggggcatga atacagggag agcccgatac cgggggaaga
     1681 agggactttc aacgttagga aaggagaaga ctttttatat cacgatgatg aggatgattg
     1741 gtatgggttt tacgattcgt tttatttcaa gcggtccgag cttgtgagct ttttcccaga
     1801 actggaagat cacaacattg atgaaccgat agcagaggaa acagaaagtg ggtcgctccc
     1861 cagtgactgg cgcggcaaaa acaccgcctg gaaaatgatt gctgggctgg cgattgcttt
     1921 atatgaatcc agcgaggacg ttagaaagaa tggaaagctc aaccagtccg cggtgtgcga
     1981 gaaagcagca aacaacatcg caaagtacag tgaagaatac aatgatgctg gcatgaccat
     2041 ggataatcta agaacgcttc taaaaaagac tttgcagcgg cacgcaccca attttttggg
     2101 aaagataacc aagtaaaata atcgatgttc ccaaattggc tggagagacg aaaacaaacg
     2161 cccaatttgg gacaattcct ttcccgaaaa ctttttctcc aaattcacag aaaacttaaa
     2221 aaaaacaatg atttactatc tctcatagtc aagcaacgta ttacgaggta gatatgaaaa
     2281 agtcgctgat tcggctatct gaggttttga aacgcactgg ctatagcaag gcgtgggtat
     2341 atgctctgat gagccgagga caattccctc aatcagtcaa gattggtgct cgcgcaatag
     2401 cttttattga aagcgagatc gatgactgga tcgatcaacg tatagccgaa tcgcgtagta
     2461 actaaagaac agaaacaaac cggatacaaa caatacgtta ttaggtatta agaatgcagt
     2521 tgacccccac caatcaaaag ctgaacgtaa cccatgttga ttttgagacc ttcgcggaac
     2581 tgattagatc agatccacaa aaggtaagca ccagcacctg ggtttatgat gtgaaatcca
     2641 ccaacggtgg aaaagctatc ggcattgtct ctggcgatga ttacttagta atcgatctga
     2701 tttaatcgca caaaaattgt agagtgattt gggaagacag gaagtcttcc tccgtattgc
     2761 attttggtac aacagtaagg gaataaaatg attaattatc tgaccgctaa agacgcagca
     2821 gaatatcttg gtgtctgtct atcacgcttc tatcagttaa agcgatatat ccccagcttt
     2881 cccccctatg taaatcagac catcaatgga cgcaagcgca gagtgtggct tgcctccagc
     2941 cttgatcaat ttgccgacag atttctgggg ggacgaaaat gagcactctt acacgtactc
     3001 aggtcgccgc aaatatacgc gatagcctac taagtggcag gaagcttacc ccaaaggagt
     3061 ttgacgagat attaaggaaa gctggaaatc acgaacgtag tcgcgtttta acgctgttac
     3121 gcaacgattg gggaattcct gttgagcagt ttaaaacagg ggcgtatcac gtcacagaac
     3181 gcaatttaga agcataccac agcgataaag acgaaacatt gaaaatctgg aggacgaacg
     3241 ccagatatgt aaagacattg cgtaaggtaa acataacgtt gtcgttgttg cgtgggctgg
     3301 tgggcaaagt gcctgaagac acgcttagaa ctatttacaa gggtattgaa accaaatacc
     3361 tgtgattgac aaagcagaaa gccgactttg cgagagtcgg ctttgaagca attgaaatat
     3421 atgaacttta cacatggaat tatacgataa agacgcgagt ctatccacaa aatcacaagc
     3481 cgtaatcaat cgcatcaatg gtcgcacatt agcttatgtg aaatcagtta cgtacaaacg
     3541 taatgaatta aaggcggaga ataaacgcca atcatcaaag gctaaccact gcataccacc
     3601 agcctggaca aagaagaaat ggcgtacaat gaggcgagct ttgtttactc tggatgtcga
     3661 atcaactagt ggtatcccct actcctttac cttcagaccc gttaagggaa caacgccaga
     3721 gaaattcaac aaagcgttaa aggcacttac cagatggttg acttcgaaag gcaatcagtg
     3781 ggtttacgtc atggagtggg ctcgacacca gtcagagccg catgtacatg caataatcat
     3841 gatgaacgaa agaagcatta atgggcaaac tggcaaaatt atcagcaaat gggtaacact
     3901 ggcaaacaat gcaatcccat caaatgttcc tccccagtgg gatctagatt tgaaaggtca
     3961 gcaatattca gagcctacaa agtgtatagg tagacagatt gattacatgg caaagcccga
     4021 aactaagtta aatgacttta atagagaggc gagcaagagc ctatatgatt ggtcagatgc
     4081 caacgtttgg ggttattcga atggctgggc gcggcatgag ataaaaaaag aaacactttc
     4141 tatcactggc tttcatgcag taagacgcat actaaaagcc gttcaggcat caaggaaaga
     4201 caactacaaa gctaagcgac agatacagcg tacattgaaa cgacaagaca taaatcgctc
     4261 accaatcacc ccgttccaag caccgttaat tagcaaacac gattgggatt tatcagtgat
     4321 agcggtcacc aaagaggcag agacagagcg cagggagtta ctatcttcac agataataaa
     4381 ctcgaacaaa gtacaaaccc acccgctgtt aaggatgtta agaaaagcac gagtgaaagc
     4441 atccagagcc agaagagaaa gcggaggtta tgacctttac tactagtagt gacagatcgt
     4501 gacaaggcag agcaaaaaac aaccaacaat aagagaaata tgcctgccaa acagccaaaa
     4561 gcgcggagcg cggcaaacgc cgcgagaggc gcaagaaggc gaacctatcc attgaaacgg
     4621 tcagaagagc gcatcgcgct cgatgaccga agtccccact aacaccgtca acgaaagatc
     4681 aaagtcttag gaaaaaatga tgtttctttg atgggcttcc atccgtaaaa atcaaatttt
     4741 gagaaaaagt acttatagaa attaatcact ctgtaaggat aagttgatgt tagtttgaag
     4801 tgaag
//
LOCUS       DAZFFG010000217         4771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFFG010000217 DAZFFG010000000
VERSION     DAZFFG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.218"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(70..831)
                     /gene="kduD"
                     /locus_tag="ACHJ7J_004845"
     CDS             complement(70..831)
                     /gene="kduD"
                     /locus_tag="ACHJ7J_004845"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO5002946.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTVLGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(861..1697)
                     /gene="kduI"
                     /locus_tag="ACHJ7J_004846"
     CDS             complement(861..1697)
                     /gene="kduI"
                     /locus_tag="ACHJ7J_004846"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO5002947.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1984..3165)
                     /gene="yqeF"
                     /locus_tag="ACHJ7J_004847"
     CDS             complement(1984..3165)
                     /gene="yqeF"
                     /locus_tag="ACHJ7J_004847"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO5002948.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVIDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3420..4649
                     /gene="yqeG"
                     /locus_tag="ACHJ7J_004848"
     CDS             3420..4649
                     /gene="yqeG"
                     /locus_tag="ACHJ7J_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5002949.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGIKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1246 a         1218 c         1039 g         1268 t
ORIGIN      
        1 ggattatgat gatctacaga tataaaaaac cctgccatgc agcagggtca taaaagtaag
       61 aagaatgaat taacgcgcca gccaaccgcc atccacggca atggtataac cattcacata
      121 atctgaagcg ctggaggcaa ggaacactac cggccccatc aggtcactcg gcagtcccca
      181 acggccagct ggaatgcggt cgaggatttc cgcgctacgt tgttcatctg cccgtagttg
      241 ttgagtattg ttggtcgcca tgtaacccgg ggctatcgca ttaacattaa tgttgtgttt
      301 agcccattcg ttcgccatca atcgcgtcac gcccataaca ccgctttttg atgcggtata
      361 agaaggcaca cggatcccgc cctggaagga gagcattgac gcgatattga taatcttgcc
      421 gccattgcct tgcgcaataa agtgtttcgc cgctgcctga gacatgaaga atacgctctt
      481 gatattcagg ttcatgacat cgtcccagtc tttttcgctg aactcgagag catcttcgcg
      541 gcgaatcaat ccggcgttat tcaccaggat atcaatatga ccaaactcag ctaccgcgcg
      601 atccagcagt gctggaatgc catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg
      661 acgccccagc accgtgacct gcttgatggt ttcagtcggt tcaacgatgt taatgccaac
      721 aatgtcacag cccgcttgcg ccagccccaa cgccatcccc tggcccagtc cagtatcaca
      781 accagtgacg accgcaactt taccttcgag agaaaatgca cttaaaatca taacaatacc
      841 ttattcgttt atgcccacaa ctagcgcaaa tctttaacgg ccacatggtc catatcatca
      901 aagacctggt tttcaccgac catgccccag ataaaggtat aagctttggt tccgacgccg
      961 gaatggatcg accagctcgg ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc
     1021 gtttcttgcg gctgccccat catgtggaaa acgcaggcgt catcatccat attgaaatag
     1081 aaataaactt ccatccggcg ctcgtgggtg tgacacggca tggtgttcca caagttacct
     1141 ggagccagct ccgtcagccc catactcaat tggcaggttt ccagcacatc cgggacaaaa
     1201 tatttgttaa tcgtgcgacg gttactggtg aggttatcac ctaacgtgac aggagatact
     1261 tcgtccggtg tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa
     1321 aacttcgccg gagtgccggt atcaatactg gcaaagacaa cttcttttgc acctttaccc
     1381 acatacaggg cgtcgcggtg accgatttca tagcattgac catcgacagt aatcgtaccg
     1441 gcaccgccaa tattgataac acctaactcg cgacgttcaa ggaaataact tacgccgagt
     1501 tgtttaccaa cttccccgcc aacggaaacc gttttagtta ccggcataat gccgccaaca
     1561 ataattcggt caatgtggct gtaaaccatg gtgtactcat cggcgacaaa taccttttca
     1621 accaaaaatt cattgcgcag cccttgggta tccagtgttt ttgcgtgcgc actgtggatg
     1681 ctctgtctta cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat
     1741 agtgaaacac tgtttcgttt tctctataag gggatgatat gcccatggga tagggttttc
     1801 aattaaaaat aaaacaatgt ttcactttta agttaaggat taaaaaaagt gcagtgtcga
     1861 tcacgaatat gtcatgagaa tggaaaaatc agggagtcct gaaaaggtat gtcgactcac
     1921 cagccatcat tattcaacag attaatgacg gcagatattt atcggtaagg aggaaatgca
     1981 gagctactcg tcacgttcaa tggtcaatgc tacgccctgg cccccgccga tacaaagcgt
     2041 tgccagtcct ttgcgggcat tacgtttcac catttcatga accagagaaa ccaggattcg
     2101 gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga cattgacccg
     2161 acgctcatcc cattcaagca tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt
     2221 agcctcgata agatcgacat cagccaactg ccagcccaca cgctccaggc aacggcgggt
     2281 cgcatacacc ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg
     2341 gatgcgggcc agcacgggta aattcaacgc tcgtgctttg gcttcgctca tcatcattac
     2401 cgcagctgcg ccatcgttta tggatgatgc attacccgct gtcacagaac cgagactatc
     2461 aaatgaagga tttaaacggg ctaagccttc tgcactggcg tcagtgcgtg gctgttcatc
     2521 ggtatcaata accaacgtct gaccattact ttgggtcatt accgggacga tctcatcttt
     2581 aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc
     2641 ctgcaactga cggctgatgc catattcgcg agccagattt tcggcggtga caccaatatg
     2701 ataatcattg aaggcatccc acaacccatc atgcaccaga ctgtcaacca actggctatt
     2761 gccaagctgt gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc
     2821 ctggccaccg gcgatgacaa tatcagcctc gccacactgt atcgcctgag tagccagatg
     2881 cagtgcttta agcccggaac cgcaaacgtc attaatcgtg attgcagaaa cgctattagg
     2941 aagaccacct ttaatagccg attgccttgc cggattctgc cctgcccctg cagtcaacac
     3001 ctgaccaaga attacttcat ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc
     3061 tttcacgacc agactaccaa gttccacggc ggaatgaccc gctaacgcac cacgaaagca
     3121 gccgataggt gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa cctcgcacca
     3181 attaacagtg caatagtaaa tgattgttaa ttacagttat ctttattgtt taaaaaaagt
     3241 gatttttatc acaaaggaaa tatgcctgag cagcagtcag agacataact ggcacgtaag
     3301 gtttgcaacc actaacccac caatagaggg gtagataggg cgttaatctc ccatacttaa
     3361 cctggtttat ggtaaattgc cctctatttt gtttaatttg tagatgatac gttcagataa
     3421 tgtctaatat ttggtcaaaa gaagaaactc tgtggagttt cgcgctctac ggcacagccg
     3481 ttggtgcagg cacgctcttc cttcctattc agttaggttc ggcaggggca gtggtcctgt
     3541 ttattactgc tctggtcgcc tggcctttaa catattggcc acataaagcc ttatgccagt
     3601 tcatcctctc atcgaaaaca tcagcaggtg aagggataac gggcgcagta acacactact
     3661 atggcaagaa gattggtaat ctgattacca cgctgtactt catcgccttt tttgtcgtcg
     3721 tgttgatata cgcagtggca attaccaact cacttacaga acagctggca aagcatatgg
     3781 tgattgatct tcgcatccgt atgttggtga gtctgggtgt tgtattaatc ctgaatctca
     3841 tttttctgat gggacgtcat gccactattc gggtaatggg atttttggta ttcccattga
     3901 ttgcctattt cttatttctt tccatttacc ttgtcggtag ttggcaacct gatctattaa
     3961 caacccaggt agagttcaat cagaataccc ttcaccagat atggatatcg attcccgtga
     4021 tggttttcgc ctttagccat acgcccatta tttctacgtt tgccatagac agacgtgaaa
     4081 aatatggcga acacgctatg gataaatgca aaaaaattat gaaagtcgct tatctcatca
     4141 tctgcataag tgtactgttc tttgtcttta gctgcctgct ttctattcca ccttcgtata
     4201 ttgaagcggc taaagaagaa ggggttacca ttttatcggc gctttctatg ctgccgaacg
     4261 ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt agttgccatg tcgaaatcat
     4321 tcctgggtac gtactttggc gttattgaag gtgccacaga ggtcgtcaaa acaacactac
     4381 agcaggttgg tataaagaaa agtcgtgcat ttaaccgcgc actatcaatt atgttggtat
     4441 cgctgattac cttcattgtt tgttgcatta acccgaacgc gatttcgatg atttatgcga
     4501 tcagcggccc gctcattgcc atgatacttt tcatcatgcc tacgctatca acatatctca
     4561 tcccggcgct taaaccctgg cgttccatcg gaaatctgat tacgctgatc gtgggtatcc
     4621 tgtgcgtatc ggtaatgttc tttagctaat atccattgtg aatggttcaa aattgctaca
     4681 attttgaacc attccatttt tcataatgtt catttgttat tattccgccc tatcacttct
     4741 ctcgtcatat cctagaatta tcagttagtt a
//
LOCUS       DAZFFG010000218         4751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFFG010000218 DAZFFG010000000
VERSION     DAZFFG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.219"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(442..1980)
                     /gene="aldB"
                     /locus_tag="ACHJ7J_004849"
     CDS             complement(442..1980)
                     /gene="aldB"
                     /locus_tag="ACHJ7J_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO5002950.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2088..3383)
                     /locus_tag="ACHJ7J_004850"
     CDS             complement(2088..3383)
                     /locus_tag="ACHJ7J_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO5002951.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(3513..4664)
                     /gene="yiaY"
                     /locus_tag="ACHJ7J_004851"
     CDS             complement(3513..4664)
                     /gene="yiaY"
                     /locus_tag="ACHJ7J_004851"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO5002952.1"
                     /translation="MAASTFFIPSVNVIGADSLADAMNMMAEYGFRRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSIIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1141 a         1257 c         1158 g         1195 t
ORIGIN      
        1 acttcctgaa gtgtgctgcc tggcttttta acaggtcaag gcttatcata ttgaaataat
       61 ggaactattg gcaaaattta tgattaaaaa ccgtaaaaca tccacaatgc agattaagcg
      121 tattaaacct tcaataatct accgtttatt gctgattgtc ctcggaatac caatgatgat
      181 ttacggtctg gtttgcccgc tcaccatcga aacgcgagat taaactcctg gcaaaacacg
      241 ccccagaaac gcacttctgt tggcgtagtc tgggttattg cgcacgtagg tttaaaacgt
      301 aaattgtaac aacgtgcgct ttgtttatgc cggatgcggc gtgtacgcct tatccggcct
      361 acggtcaggt ccccgtaggc atgataagac gcgtaagcgt cgcatcaggc aattaatacc
      421 cgatgcgacc agcttcttac atcagaacag ccccaacggt ttatccgagt agctcaccag
      481 caggcacttg gtttgctggt aatgctcaag catcatcttg tgggtttcgc gtccaatgcc
      541 tgattgtttg tagccgccaa acgccgcatg tgccgggtaa gcgtgataac agttggtcca
      601 cacgcgcccg gcctgaatgc cgcgccccat cttataggcc agattaccgt tgcggctcca
      661 gacgcctgcg cccaggccat attgcgtatc gttcgccagc tccagcgctt cttccatcgt
      721 tttgaaggtg gtcaccgcca gcaccgggcc aaaaatctct tcctggaaca cccgcatatt
      781 gttctgacca aacagaatcg tcggttcgag gtagtagccg tctttcagtt caccttccag
      841 cagcttgcgc cgcccgccgg tgagcacgtc tgcgccctct tttttaccga tatcaatgta
      901 gttgaggatg gtttccagtt gcccgtgaga aacctgcgcc cccatttgcg tcacgctgtc
      961 gagtgggtta ccgctgcgaa tgctttcgac acggcggatg gcgcgttcca taaagcgttc
     1021 gtagattgac tcctgcacta aggcacgact cggacaggtg caaacttcgc cctggttaaa
     1081 ggcaaacagt gcaaagcctt ccagcgcttt atcgaaaaag gcatcttctt catccatcac
     1141 atcagcaaag aagatatttg gcgacttacc gcccagctcc agcgtcaccg gaataatgtt
     1201 ttgcgttgcg tattgcataa tttgttggcc cacttccgtt gagccggtaa acgccacttt
     1261 ggcgatgcgt ttcgaggtcg ccagatattc gccaatttcc ccacctgcgc cattgaccac
     1321 gttcaccacg cccggcggca gtaaatcacc gacaatttcc attagcagca gtacagaaag
     1381 cggggtaaga cgtgcgggtt tcagcaccac acagttgccc gccgccagcg cgggagccat
     1441 tttccagctc gccatcagca gcgggaagtt ccacgggata atctgcccca ccacgcctaa
     1501 cggttcatgg aaatgatagg ccacggtttc gctatcaact tcactgatcc caccttcctg
     1561 cgcccgaata cacgaggcga aatagcggaa atggtcaatc gccagcggca cgtcggcagc
     1621 actggtttcg cgaatgggtt tgccgttatc ccaggtttca gctgtcgcta acagctcgag
     1681 gttttgttcc attcgatcgg caatcttaaa cagaattgcc gcgcgatcct gcaccgaggt
     1741 atgtgcccat ttatctttca ctttatgcgc ggcatccagc gccagatcga tgtctcgttt
     1801 gcccgaagac gccacttcgc acagtagctg cccggttacc ggcgtcaggt tctggtaata
     1861 ctcaccgtcg gcaggggcta cccattcgcc gccaataaag ttgtcatagc ggttttttaa
     1921 cttgatgggg aaaccatact cgccgggctt aatctgtgct gaagggggat tattggtcat
     1981 gatcgtctcc ttgcggtgtg aggtataaca agggtagacg tggctggcga aatcttcgcc
     2041 agtcggtaac agctttacga cggctatcac gaatttacgg gcaagggtta cagcggataa
     2101 tgcgcgaagt acacggcttg ctggtcatta attttcttct cttgcgcaaa cccttcttta
     2161 acaattgtcc gtaaatcagc tcgagccgta ttatcagaga cgcccaggct cgcgctaatt
     2221 tcagcaactg tgtactcttt ccccggattg gcgagcatca cattcaggag cgttacctgg
     2281 cgcggattca ggcgtttaag cacaccagag ctaaacagca cactatcgag ttttgcgctt
     2341 cgttgtgtaa tttcatcgat atgctgctgc cagttttgca gagcacgttt aatgacaccc
     2401 gcctgatatt caaggaaata agtcagatcc ataccatctg attcagtgta ctggtagctg
     2461 gcggcatatt tgaccggtgc agcatgaagc aaacggctga tggaaatata tttaaagaca
     2521 tcgtagcccg atttcagcat ataccagtaa aacagcgccc ggctggtgcg cccgttgcca
     2581 tcccgaaacg gatgttcatg ggccagcata aaatgcagaa tgcaggcacg gaccaacgga
     2641 tgaatatacc cttcatggct gtttaaccac tggcaaactt tttccagccg ttccggtaac
     2701 aatgccgcag cgggcggttg atgcactata ttgccatcat agtcggcgat cacaacatca
     2761 tctgtttcac gaaattctcc cggacgatat ttcgcgtcat tgattccacc cataccaatg
     2821 gcatgtaact ggcgaataag cgcaggtgtc agcggttctg cgagtaagtg cggaatttcc
     2881 gccatcagcc gggcatttcc cgcaatcata tgctcgtctt cagtgcgtgg cgaccgtcct
     2941 gtttccagca tttttctcgc caccagcgtt gtagtattcg caccttccag ctgggagctg
     3001 gtgatcggct cttccaggcg taactggctt aactcggctc cggccccgcg taaccgttca
     3061 gtctgatctt tcagcgccag tgcagtggca cgtttgtcga caagctcgca cgcttctgca
     3121 atcaccgggg taatgttgaa tccggcctgt tcacccgctt cattgcaata attaattcgt
     3181 tggatcgccg cgttgcgcgc cagacgggta agcgtccacg ccacatcgac gttttctcct
     3241 tttttgatgc ggtactgcaa ctcatcaaaa ggcagataac gtcctttttc atcggtaacc
     3301 cggttaaagc gcaggtgggc caggagttcc tcacccgata agtgtgataa aggaataagt
     3361 ccgggaggtg atttgatagc catgtgctta tctcaaaaaa gcgcaatatt gagatagtgt
     3421 atagcagaaa agagaaacta tctcaaaaaa agcaagtttt gagatagttg aaaaagcggc
     3481 tgacaatttg ccagccgttg tggaaatgat gattacatcg ctgcgcgata aatcgccaca
     3541 atttcttcgt gagttgcctg gatcgggtta gtaaagccac aggcatcttt cagggcatta
     3601 gtcgccagaa ccgcgaaatc ttcttctttc acgttcaggt cgcgtaggcc tgccgggata
     3661 tccactttct tcgccagttc acggatggcg ttaatgcagg cttcagcacc ttccgcgtcg
     3721 tttttacctg tcacgttcac gcccattgca gcggcacagt cacgcagacg tgcagcggcg
     3781 actttgctgt tgaatacctg aacatgcggc agcagaacgg cgttacatac accgtgtggc
     3841 aggttgtaga aaccgcccag ctggtgcgcc atcgcatgaa cataacccag agaagcatta
     3901 ttgaacgcca taccggcaag gaactgggca taagccattg cttcacgcgc tttcgcatta
     3961 ctgccatctt caacggctaa cggcaggttt tcggcaatca tggtcacggc tttcagtgca
     4021 caagcgtcag tgatcggcgt ggcggcaata gaaacatacg cttcgatagc gtgcgttaag
     4081 gcatccatac ccgttgcggc ggtcagtgac ttcggcatac caatcatcag agaggagtca
     4141 ttgacagaaa gcagcggagt gacatgttta tcaacaatcg ccattttgat atgacgcgct
     4201 tcgtcagtga tgatgcagaa acgggtcatt tcagaggccg tacccgccgt ggtattgatg
     4261 gcgatcatcg gcagctgcgg ttttgcagag cggtcaacgc cttcgtaatc gcgaatatcg
     4321 ccgccattgg ctgccaccag cgcaatacct tttgcgcagt cgtgcggaga accaccgcct
     4381 aaggagatca cgctatcgca attattttct ttaagtaatt tcaaacctgc ggcgacgttt
     4441 tccgtggtgg ggttaggttg ggtaccatca taaataatgc taaaaatatt gcgttcttcc
     4501 agtgcttttt gcacatcgcc cgccatacct aatttcgtta acatactgtc agtgacaatc
     4561 aatgtacggc gaaacccata ttctgccatc atattcattg catcagccaa tgaatcagcg
     4621 ccgatgacat tcacagaagg aataaaaaag gttgaagctg ccataacaca ctcctgaaag
     4681 tgtaaaaaag ggcgggcagc ttactgagga ttttcattcg aaaatatgat cagtattatt
     4741 gtttagcaaa a
//
LOCUS       DAZFFG010000219         4673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFFG010000219 DAZFFG010000000
VERSION     DAZFFG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.220"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>4673
                     /gene="cdiA"
                     /locus_tag="ACHJ7J_004852"
     CDS             <1..>4673
                     /gene="cdiA"
                     /locus_tag="ACHJ7J_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFO5002953.1"
                     /translation="APSLSLSAPELTSSGVLVGSALNTQSQTLTNSGLLQGEASLTVN
                     TQRLDNQQNGTLYSAADLTLDIPDIRNSGLITGDNGLTLNTASLSNPGKIIADTLNVR
                     ATTLDGDGLLQGAGALALAGDTLSQGRNGRWLTTDDLSLRGKTLHTAGTTQGQNLTVQ
                     ADNWANSGSVLATGNLTASATGQLTSTGDIMSQGDTTLNAATTDNRGSLLSAGTLSLD
                     GNSLDNSGTVQGNHVTLHHRSTDNSGTVTGLSGLTLHSADGLTNSGALLSQNSLALSA
                     GDVTNSGRIQGQNITLDASSLTSSGAVQSALDLALTLSGDVIAATGSKITAMGDARLT
                     GKVLGNQGLISAKTLEVNGDSLSNSGEISGVNSLNVTLSGNLQQHGKMLTGGALNVNA
                     RDISNSGQLQGADNRITASSLTNSGRVQGDSGLTLTLLNALTNQTSGVLLSQNVSALS
                     APVLTNDGTIQGNGKTTLSAATQAHNSGKILSGGELTFTTPDYSGSGWLQATDLLLNV
                     AKLAGNGTVMAANQATLTGNSLTNRGLFQAAQLNVNTQTITNSGTLLGNQGLTIKGNS
                     LNNAGGKVFSGGDMLADMVSLSGAGQLVALGNLTLKLTRGLTAQGVIAANKQLSVSSQ
                     GDITNGATLQGNGITLNAAGRLTNNGQLTAGNGTTALSGSGIAMNASGSLQAGGDVSL
                     TSRGDITLDGFTGTTGSLMLTAAGSVINTALLYAGNNLSLFASTIRNHHGDMLAGDSL
                     AMQKDVSGAANAEVINTSGNIETTRGDITIRTGHLLNQREGINETKSYIPVENVAVPD
                     GANSVSVRVGDLGEDGWGYYVKSWSGTAGGGFEAWAVPTEKGATRKFLTGTTRVDVGA
                     TGGDARISAGNNLLIDADKLDNTGSHLLASEFVSLSGSQLNNQSFFGYTQDEYNVYRY
                     YGKLAMIPNDGHLQYGDASADDRVTFTLSGAPEYVTRDTGQALRAVIQAGKNVTAVFS
                     SDISNTSTTSNAGRITNTLAAPEINTPAEKNISPGMAQLAPDGTETLTVTAPDWTDTI
                     TRLTIGSGTDLASGIVVGNYPLPSGNNGYFVPSTDPDSPYLITVNPKLDGLGKVDSSL
                     FAGLYDLLRMHPGQAPRETDPAYTDDKQFPGSSYFLDRLGLKPEKDYRFLGDAAFDTR
                     YVSNYMLNQIGGRYINGVGSDTDQMRYLMDNAARAQKALGLKFGVALTADQVAALDQS
                     ILWYKAVTIKGQTVMVPEVYLSPKDVTLQNGSIISGQNVHLAGGNVTNSGSTLMAQND
                     LTIDSADSLGNLESGLINAGGALGLKAMGDINNISATITGKTVRLESLAGNVNNLTRY
                     SHWQLDAPEDSLALKHTYTGSIASVSAMDSLDIRADKNISVTGAEISAGGRAALIAGN
                     DLSLNAIDRVSSSRHANSESHQRSAELTTITAGDSVMLSAGRDVSSQGAGIAAEDNIT
                     VRAGRDVNLLAEESVTGSSSYSKKKTVIDETVRQQGTEIASGGDTTITAGRDITAVAS
                     SVTATGNISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKTTRTISEDSATREAGS
                     LLSGNR"
BASE COUNT         1142 a         1271 c         1363 g          897 t
ORIGIN      
        1 gccccgtcgc tcagcctcag tgcgccggaa ctgaccagca gcggcgtact tgttggttct
       61 gccctgaata cacagtcaca gaccctgacc aacagcggtc tgttgcaggg ggaggcctca
      121 ctcaccgtta acacacagag gcttgataat cagcagaacg gcacgctgta cagtgctgca
      181 gacctgacgc tggatatacc ggacatccgc aacagcgggc ttatcaccgg tgataatggt
      241 ttaacgttaa acactgcctc cctcagcaat ccgggaaaaa tcatcgctga cacgctgaac
      301 gtcagggcga ccacgctgga tggcgacggc ctgttgcagg gcgccggtgc actggcgctt
      361 gcaggcgaca ccctctcaca gggccgtaac gggcgctggc tgacgacgga cgacctctcc
      421 ctccggggca aaacactgca taccgcaggg accacgcagg gacagaatct caccgtgcag
      481 gcggacaact gggcgaacag tggttccgtg ctggcaaccg gtaaccttac tgcttcggca
      541 accggtcagt tgaccagtac cggcgatatc atgagccagg gggacaccac gctgaacgca
      601 gccaccacgg acaaccgggg cagtctgctt tcggccggca cgctctctct tgatggaaat
      661 tcactggata acagcggcac tgtccagggt aaccatgtca cgcttcacca cagaagcact
      721 gacaacagcg gtacggtcac ggggctgagc ggtctgaccc tgcacagcgc ggacgggctt
      781 accaacagtg gcgcgctgct gagccagaac tcgctggcgc tgagcgcagg agatgtgacc
      841 aacagcggcc ggattcaggg gcagaacatc actctggatg ccagcagcct gaccagcagc
      901 ggtgctgttc agagtgcgct ggatttggct ctgaccctga gtggcgatgt tattgctgcc
      961 accggcagca aaattaccgc catgggcgat gcccgcctga ccgggaaagt gctgggtaac
     1021 cagggattaa tcagcgcgaa gacgctggaa gtgaatggtg attcgctcag caacagtggc
     1081 gaaatcagtg gtgttaacag cctgaacgtg acactcagtg gtaatctgca acagcacggt
     1141 aaaatgctga caggcggtgc attaaacgtc aacgccagag acatcagcaa tagtgggcag
     1201 cttcaggggg cggataaccg aatcaccgcc agttcgctga ccaactcagg tcgtgtgcag
     1261 ggcgacagcg ggctgactct tacattgctg aatgccctga ccaaccagac cagcggtgtg
     1321 cttctcagcc agaacgtatc agcgctttcc gcacccgtgc tgaccaatga cggcaccatt
     1381 cagggcaacg gtaaaaccac gctcagcgcg gcaacgcagg cccacaacag tggcaagatt
     1441 ctttccggcg gtgagctgac gttcaccacg ccggactaca gtggcagtgg ctggctacag
     1501 gccactgacc tgctgctgaa cgtggcgaag cttgccggta atggcactgt gatggcggcg
     1561 aatcaggcaa cgctcacagg taacagtctg accaaccgtg gcctgttcca ggccgcacaa
     1621 ctgaacgtca acacgcagac catcaccaac agcggcacgc tgctgggtaa tcagggcctg
     1681 acaataaaag ggaacagcct gaacaacgcc ggcgggaagg tattcagcgg cggtgatatg
     1741 cttgctgata tggtctcgct cagcggcgcg ggccagctgg ttgcactcgg taacctgacg
     1801 ctgaagctca cccgtggttt gacagcacag ggcgttatcg ccgcgaataa acaactctcc
     1861 gtcagcagcc agggtgatat taccaatggc gccacattgc agggcaacgg tatcacgctg
     1921 aatgcggcgg gcaggctgac caacaatggc cagttgaccg ccggcaacgg caccacggca
     1981 ctgagcggca gcgggattgc gatgaatgcc agcggcagcc tgcaggcagg cggggacgtc
     2041 agtctgacca gccgggggga tatcactctc gacggtttta ccggcacgac gggtagtctg
     2101 atgttaacgg ccgccgggtc agtcattaac accgcactgt tgtatgcggg caataacctc
     2161 tcgctgtttg ccagcaccat ccggaaccat cacggcgata tgctggcggg tgacagcctg
     2221 gcgatgcaga aagatgtcag tggtgccgcg aatgctgaag tgattaatac ctccgggaat
     2281 atcgaaacca cccgcggtga tatcaccatc agaaccggac atttactgaa tcagcgggag
     2341 ggtataaatg agacaaaatc atacattccg gttgagaatg tcgcagtacc tgatggtgca
     2401 aattctgttt cagtcagagt gggcgattta ggtgaagatg gatggggata ttatgtgaag
     2461 tcctggagcg gtactgccgg tggtggtttt gaggcatggg ccgttccgac tgaaaaggga
     2521 gccacccgga aatttctgac tggcacaaca cgcgtcgatg ttggtgcaac agggggggat
     2581 gccagaattt cggccgggaa taatctgctg attgacgcgg acaaactgga taatacggga
     2641 agtcatcttc ttgccagcga gtttgtcagc ctgtcgggtt cacaactgaa taatcagtcc
     2701 ttctttggct atacacagga tgaatacaat gtgtatcgct attacggcaa actggccatg
     2761 atccccaacg acgggcatct gcagtatgga gatgcttctg ccgatgatcg cgttaccttt
     2821 actctctccg gagctccgga atatgtcacc agagacaccg gtcaggctct gagagccgtg
     2881 attcaggcag gcaaaaatgt cactgccgtt ttttccagtg atatcagtaa tacatccaca
     2941 acctcaaatg caggacggat cacaaatacc cttgcggcac ctgaaattaa cacgccggca
     3001 gagaaaaata tcagcccggg aatggctcaa ctggcgccgg atggcacaga gacgctgacc
     3061 gtcacggccc cggactggac tgacaccatt acccgtctga cgatcggcag tggtacggac
     3121 ctggcttccg gcatcgtggt ggggaactat cccctgcctt ccggtaacaa tggttatttt
     3181 gtcccgtcca cggacccgga cagtccgtat ctgattacgg tgaatccgaa actggatggt
     3241 ctcggaaagg tggacagcag tctgtttgcc ggactgtatg acctgctcag gatgcacccc
     3301 gggcaggcac cacgtgaaac agacccggcg tatactgatg ataaacaatt cccgggctca
     3361 tcgtattttc ttgaccggct cgggctgaaa ccggaaaaag attatcgctt cctgggggat
     3421 gccgcttttg atacccgcta tgtatcgaac tatatgctga accagatcgg ggggcgctat
     3481 atcaacgggg ttggttcaga caccgaccag atgcgctacc tgatggataa cgctgcccgt
     3541 gcacagaagg cgctcggtct gaagtttggt gtggcactga cagcagacca ggtggcggct
     3601 ctcgaccaaa gtattctgtg gtataaagcc gtcacgataa agggtcagac ggtgatggtg
     3661 ccggaagtgt atctgtcacc gaaagatgtg acgctgcaga acggcagtat tatcagcggt
     3721 cagaatgtgc acctggcagg agggaatgtc actaacagtg gcagtaccct gatggcgcag
     3781 aatgatctca ccatcgacag cgctgacagt ctgggtaacc ttgagtccgg gcttataaat
     3841 gccggtggcg ctctcggact gaaagcgatg ggcgatatca acaatatcag tgccacgatt
     3901 accggtaaga ctgtccggct ggagagtctc gccggcaatg tcaataatct cacccggtac
     3961 tcacactggc agcttgatgc accggaagac agcctcgccc tgaaacacac ttatacggga
     4021 agcattgcct ctgtcagtgc catggatagt ctggacatcc gggcagataa aaacatttcg
     4081 gtcacagggg cagaaatcag cgccggaggt cgcgcagcac ttattgccgg caatgatctc
     4141 agtctgaatg ccattgacag ggtaagcagc agtcgccatg caaacagtga atctcaccag
     4201 cgcagtgcag aacttacgac cattactgcc ggcgacagtg tgatgctgag tgcaggccgg
     4261 gatgtgagca gtcagggtgc aggtatcgca gcggaagaca acatcactgt ccgggccggg
     4321 cgcgacgtta atctgctggc ggaagagtcc gtgaccggca gcagttcata ctcgaaaaag
     4381 aaaacggtca ttgatgaaac cgttcgccag cagggaacgg aaatcgccag cggtggtgac
     4441 accaccatta ctgcaggacg ggatatcacc gctgttgcgt catccgttac cgcaaccggc
     4501 aatatcagtg tgaatgccgg tcgtgatgtc gccctgacca cggcgacaga aagtgactat
     4561 cactatctgg aaacgaagaa aaaaagcgga ggttttctca gtaagaaaac cacccgcacc
     4621 atcagtgagg acagtgccac ccgtgaagca ggctccctgc tgtcggggaa ccg
//
LOCUS       DAZFFG010000220         4662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFFG010000220 DAZFFG010000000
VERSION     DAZFFG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.221"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            164..411
                     /locus_tag="ACHJ7J_004853"
                     /pseudo
     CDS             164..411
                     /locus_tag="ACHJ7J_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
     gene            complement(428..631)
                     /locus_tag="ACHJ7J_004854"
     CDS             complement(428..631)
                     /locus_tag="ACHJ7J_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO5002954.1"
                     /translation="MVGYTVLWGPGNLSDSPKLKKIIGVFTFLAPVNPHQNQFPATLW
                     RGRPQNSLKRSAIYHAHQKQERM"
     gene            657..731
                     /gene="tap"
                     /locus_tag="ACHJ7J_004855"
     CDS             657..731
                     /gene="tap"
                     /locus_tag="ACHJ7J_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO5002955.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            724..1581
                     /gene="repA"
                     /locus_tag="ACHJ7J_004856"
     CDS             724..1581
                     /gene="repA"
                     /locus_tag="ACHJ7J_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5002956.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAVHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELISKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2552..2887
                     /locus_tag="ACHJ7J_004857"
     CDS             2552..2887
                     /locus_tag="ACHJ7J_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5002957.1"
                     /translation="MIELPDNLRGRMARLIERLEQEGNKMKMPHSCVIGGGLFELIVG
                     DKNIARVEMLYEMREKAGLTKSALAERMGLRSSAISRLESNPLGASMKTLTRYAQACG
                     ATIDIHAVY"
     gene            complement(3097..3279)
                     /locus_tag="ACHJ7J_004858"
     CDS             complement(3097..3279)
                     /locus_tag="ACHJ7J_004858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002958.1"
                     /translation="MRLVDAEAIAYEKRYSYPSIDRDGKEVNGARTYQPGKLTAWQRP
                     AFPVLVEVMSKVCNRQ"
     gene            complement(4305..4649)
                     /locus_tag="ACHJ7J_004859"
                     /pseudo
     CDS             complement(4305..4649)
                     /locus_tag="ACHJ7J_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1241 a         1115 c         1091 g         1215 t
ORIGIN      
        1 cctgtttttc agtcatttcg caataaaatt cacaccaaac gacagtaatt cctgttgatt
       61 tgtgcgccaa cacagatctt cgtcacaatt ctcaagtcgc tgatttcaaa aaactgtagt
      121 atcctctgcg aaacgatccc tgtttaaata ttgaggagac gagatgttgc agacagaaaa
      181 tgcagtgact tcctcgtcag gtacaaagcg agcatacaga aaaggtaatc ctcttacgct
      241 tgcagaacgt cagcaggcat ccctagcaag aaacgtgcca cacataaaga gctcagagtg
      301 ttcattcctg cggcgttaaa agcgcaattg caggtaatgt gtgaagctga gggggttacc
      361 caggctgaga tgattgctga actgataacg cagaagagtg catttagcta attaaaatgt
      421 aacaacatca cattctttcc tgtttctgat gtgcgtgata gattgctgat cgttttaagg
      481 aattttgtgg ccggccacgc cataaggtgg cagggaactg gttctgatgt ggatttactg
      541 gagccagaaa agtgaaaacc ccgataatct tctttaactt tggcgagtca gacagattac
      601 cggggcccca taaaaccgta tagccaacaa ttcagctata cggggagtat agttatatgc
      661 ccggaaaagt tcaagacttc tttctgtgct cactccttct gcgcattgta agtgcaggat
      721 ggtgtgactg atcttcaaca aacgtattac cgccaggtaa agaatccgaa tccggtgttt
      781 acaccccgtg aaggtgccgg aacgctgaag ttctgcgaaa aactgatgga aaaggcggtg
      841 ggtttcactt cccgtttcga tttcgccgta catgtggcgt atgcccgttc gagggggcta
      901 cgtcggcgca tgccaccggt actgcgtcga cgagccattg atgcgctgct acaggggctg
      961 tgtttccact atgacccgct ggccaaccgc gtccagtgct ccattaccac actggccatt
     1021 gagtgcggac tggcgacgga gtctgctgcc ggaaaactct ccatcacccg tgccacccgg
     1081 gccctgacgt tcctgtcaga gctgggactg attacctacc agacggaata tgacccgctt
     1141 atcgggtgct acattccgac cgacatcacg ttcacaccgg cgctgtttgc tgcccttgat
     1201 gtgtctgagg atgcagtggc tgctgcgcgc cgcagtcgtg ttgaatggga aaacagacag
     1261 cgcaaaaagc aggggctgga taccctgggt atggatgaac tgatatcgaa agcctggcgt
     1321 tttgtgcgtg agcgtttccg cagttaccag acagagctta aatcccgggg aataaagcgt
     1381 gcccgtgcgc gtcgtgatgc gggcagggaa cgccaggata tcgtcaccct ggtgaaacgg
     1441 cagctgacgc gcgaaatcgc ggaagggcgt ttcacagcca atcgtgaggc ggtaaaacgc
     1501 gaagtggagc gtcgtgtgaa ggagcgtatg attctgtcac gtaaccgtaa ttacagccgg
     1561 ctggccacag cttccccctg aaagtgacct cttctgaata atccggcccg cgccggaggc
     1621 atccgcacgc ctgaagcctg ccagtgaaca aaaaagcagc acctcataca aaaaacaacc
     1681 tcaccattca ccttctggtg catccggttc ccccctgttt ttgatacaaa acacgcttca
     1741 cagacgggga atttcgctta tccacattta actgcaaggg acttctccat aaggttacaa
     1801 ccgttcatgc cataaagcgc cagccgccag tcttacaggg tgcaatgtat cttttaaaca
     1861 cctgtttata tctcctttaa actgcttaac tacattcatt taaaaagaaa acctgttcac
     1921 tgcctgtcct atggacagac agatatgcac ctcccaccgc aagcggcggg ccccaaccgg
     1981 agccacttta gttacaacac tcagatacaa ccaccagaaa aaccccagtc ccgcgcagaa
     2041 ctgaaaccgc aatgcccccc ctcataactg aaaagcggcc ccgccccggc ccaaagggcc
     2101 ggaacagagt cgcttttaat tatgaatgtt gtaactacat tatcaccgct gtcagtcttc
     2161 tggctggatg ttctcagtac acgctcgtaa gcggccctca cggcccgcta acgcggagat
     2221 acgccccgac ttcgggtaaa ccctcgtcgg gaccactccg accgcgcaca aaagctcttt
     2281 catagctgaa agcagatatg gcttagcagg gctggggatg ggtaaggtga aatctatcaa
     2341 tccgtaccgg cttacgccgg gcttcggcgg ttttactccg gtatcatatg aagcaacgga
     2401 ctgccgcctt ccatgccgct gacgcggcat caaaactgta cttaacatgt aacatgtaac
     2461 atgcatttca tttatcagtg aaatttgata tttacgcaat tttcctgttc tggtgtttaa
     2521 tgtcattact cacccggctg cactgaatga gttgattgaa ttaccagata atctacgtgg
     2581 tcgcatggcc agattgattg aacggctgga acaggaaggc aacaaaatga aaatgcctca
     2641 cagctgtgta atcggtggag gactttttga actgatagtc ggggataaaa atatcgcaag
     2701 ggtagaaatg ttgtacgaaa tgagagaaaa agcggggtta acaaagtcag cgctggccga
     2761 acgaatgggg ttacgctcgt cagccatcag ccggctggaa agtaatcctt taggagccag
     2821 tatgaaaact ttgacaagat atgctcaggc atgtggtgca accatcgata ttcacgcagt
     2881 ctattgattt gtctctgcta acaccgccag aaaaacagaa gtgaccatag gttccgttgt
     2941 cttggcaggc ctcaagatat tctggaccag gtggagagca tggaccagcg ggggcatttg
     3001 aacagaaact tacggtttca actgctgaac aaagaatgaa accgtaattt cttgaatgtg
     3061 ggtttatccg gccttggctg ttgtaataaa caaagtttat tgtcggttgc atactttact
     3121 catgacttca accagaaccg gaaaagccgg tcgttgccat gcagttagtt tgcctggctg
     3181 ataggttctg gcaccattca cttcttttcc gtctctgtca atggagggat acgagtaacg
     3241 tttttcatac gctatcgctt ctgcatcgac caatctcatt atctcctcga ctgaaaaatt
     3301 agagcacttc ctttgtgaga tctgataaat ttgctgcact gccgcataca tgtatcctac
     3361 acacagcccc cagtctgcct tactttttac tgtatctgcc tctttacaga attgatacca
     3421 gtctgaaatt ttggggtagg aggtatccac aactgtgact ccaggcatct cgatagggat
     3481 agataagatt gacgcgctag gttcagttgc aaacgtatta taggacacac tgtttagcag
     3541 gagcaggtaa aaggtaatga aaaatctagt gcttcttctg ttgcgggaca tacctattga
     3601 tctcttattt gtattgttga tttgattttt tcctggaaag gaacaaatga agataacaaa
     3661 aaatcttcat tgctcagtat attttctcca atctcatcaa aatggggcta cgtgggaatt
     3721 tctgcgacag tgcacggtaa caaaatttgg ctcattgaaa tttaatgcgt tagccattcc
     3781 gaaccaaaac attcggggtt actgaatctg aaatgtacgg aaaacgtcag aagtcagttt
     3841 gttgtatgat ttgactatcc gacggtgttt tccgcatata aaaacgctat ttttaggggt
     3901 aaatattcct ctcaccatct tctgaacgaa aaacaagtga tttccgtcag ggagaaatcg
     3961 cttagcgggt gttatcgcag aaattcccac ggagcccctg taactgtttt tcgtccataa
     4021 attaactttc ttttgatgct gggttgaaca tatcaaaatc aggcaattac ataaatctat
     4081 gtacgggatc taggtaaata atgaaaaatc cagtcagaac gcgatgtcat tacagatccc
     4141 aagtttttta taagtctgat gcttctgtaa tgaaaccgtt ttaacgcagt aaaacctact
     4201 ttctgatatc cgggtaactg aatagacttt tactaatctc atattccgtc cctgtcagtg
     4261 taagcggctc tccagaactg tattaatatt tatttagagg ttagatcaac tgtcatattt
     4321 ttgctgatga catatacatc gtggattttc cgcctggcat gggcccagca cacaacttct
     4381 gttgttagcg cgtcaccttc acctgtgaac gcagccgcct gcagaacgtc cccggaaggg
     4441 acgaaggtgt tactacaggt gttttccctg cttgtccggt gagtatacga atcagaacgc
     4501 aggaggagat gatgaccccg tattgcgatc atccaggacc tacgtccaga tgcgccctgt
     4561 tttcgtcttt ttcacacccg gtgccagtac ctttactgat gtgtcgcgtc tgtatgaagc
     4621 ttacggatgt tcattacata actgtacagg atcagttata gc
//
LOCUS       DAZFFG010000221         4647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFFG010000221 DAZFFG010000000
VERSION     DAZFFG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.222"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            161..871
                     /locus_tag="ACHJ7J_004860"
     CDS             161..871
                     /locus_tag="ACHJ7J_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5002959.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            913..3564
                     /locus_tag="ACHJ7J_004861"
     CDS             913..3564
                     /locus_tag="ACHJ7J_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5002960.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGFKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQNSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSVFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            3578..4108
                     /locus_tag="ACHJ7J_004862"
     CDS             3578..4108
                     /locus_tag="ACHJ7J_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002961.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4121..4624
                     /locus_tag="ACHJ7J_004863"
     CDS             4121..4624
                     /locus_tag="ACHJ7J_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5002962.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVMVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
BASE COUNT         1339 a         1022 c         1035 g         1251 t
ORIGIN      
        1 actttgtagc aaggttttcc tattaaaaga gaaccaacat ttattacaaa cgcattaagt
       61 ctggaaaccc ggcgactcgc caatgtatta gcaacattta ccgattgtct gatgtaacgg
      121 ttttcccgct tatcctctta acacatggag aatgcatcag atgcaaacta cgcgtacccc
      181 ctactctatt tcctttatgg cgacagtgtt acttttattg ctctttgctt gccatagcac
      241 cgttgccaac gcagctgtcg cactgggtgc gacgcgggta atttatccgg caaaccagaa
      301 acaggtttta ttgcctgtca cgaataatga tcctgccagt gtctatctca ttcagtcatg
      361 gatagaaaat gcaggtgacc aaaaagacac ccagtttgtc attaccccac cgcttttttc
      421 catgcaaggt aaaaaagaga atacgctgcg gataatcaac gctactaatc atcagttacc
      481 aggcgaccgt gaaagcctgt tctgggtgaa tgttaaagct attcctgcta tggaaaaaga
      541 ccagaagaat gagaatacat tacagctggc aattattagc cgcataaaaa tgttttatcg
      601 ccccacgaac ctggcaatgg cacctgaaga agcgcctgca atgcttcggt ttcgtcgcag
      661 cggtagcaaa ctcactctta ttaatccaac gccctacttc attaccgtaa caaatatgaa
      721 agcaggaaat agcaatctcc caaacaccat ggtgccgcca aaaggtgaag tatcggtgga
      781 tatcccgcat gctgccaccg gggatatcag ttttcagacg atcaatgatt acggcgcatt
      841 aactccgcgt attaaggcaa ccatgcagta agccagtgac ctaattaata ataaactcaa
      901 tcggaattaa taatgacagc ctttcgtgcc gcatttaagg catatcgtat gcatcaggtg
      961 ttactactgc ctcgctttgc acggcttacc attgccctta gcttagcaac tgcggtattt
     1021 cccgttgacg cagagtatta ttttaatcca cggtttttaa gcaatgatct tgctgaatct
     1081 gtcgatttat ccgcctttac taagggacgc gaggcaccac ctggtacata tcgggtcgac
     1141 atttatttga acgacgaatt tatgactagt cgggatatta cttttattac agatgataat
     1201 aacgcagatc tcatcccatg cctgagtaca gatcttcttg tcagtcttgg cataaaaaaa
     1261 tctgcgttat tagacaataa ggaacattca gcagaaaaac atgtgccgga caacagcgca
     1321 tgcacaccac ttcaggatcg tttggtcgac gcatcaactg agtttgatgt tggtcagcaa
     1381 catctgtcac tctccgttcc gcaaatttat gttggcagaa tggctcgcgg ctatgtttct
     1441 cctgatctgt gggaagaagg aataaatgca gggcttctaa actatagttt taatggtaat
     1501 tctattaata atcgtagtaa ccataatgca ggaaaatcaa actatgcata tttgaattta
     1561 cagagtggca tcaacattgg tagttggcga ctgcgcgata actcaacgtg gagttataac
     1621 agtgggagta gcaattcatc tgacagcaat aaatggcagc atatcaatac gtcggctgaa
     1681 cgtgacatta ttccgttacg ctcacggtta acggtaggtg atagttatac cgatggcgat
     1741 atttttgata gtgtgaactt tcgtggcttc aaaataaatt caacagaagc gatgttgccc
     1801 gatagccaac atggtttcgc tccggtgatt catggtattg cccgtggcac cgcacaagtg
     1861 agtgtaaaac agaacggata cgatgtttat cagactactg ttccacccgg cccttttact
     1921 attgatgata tcaactctgc ggccaatggt ggtgatctgc aagtaaccat aaaagaggca
     1981 gacggcagta ttcagacatt atatgttcct tattcgtctg ttccggttct ccaacgtgct
     2041 ggatatacgc gttatgcgct tgccatgggg gaatatcgta gtggaaataa cctgcaaaac
     2101 tcccccaagt tcatacaagg tagcttgatg catggactgg aaggaaactg gacaccttat
     2161 ggcggaatgc aaattgcaga agattatcag gccttcaacc ttggtattgg taaagattta
     2221 ggactttttg gtgccttttc tttcgatatc acgcaggcca atacgacact tgcagatggc
     2281 acccgtcaca gcgggcaatc ggttaaatcc gtctacagca aatccttcta ccagacagga
     2341 accaatatcc aggtcgcagg atatcgctat tctacgcaag gtttttataa cttatccgac
     2401 agtgcctaca gtcgaatgag tggttacacc gtcaagcctc ctaccggaga cagcaatgag
     2461 cagacacaat ttattgatta ttttaatctg ttctacagta agcgtggtca ggaacaaata
     2521 agtatctctc agcagcttgg aaattacggt acgacatttt tcagtgccag tcgccaaagt
     2581 tactggaaca cgtcacgcag cgaccagcaa atatcatttg gattaaatgt gccgtttggt
     2641 gatattacga cttcgctgaa ttacagctat tccaataata tatggcaaaa cgatcgggat
     2701 catttactcg cttttacgct taatgttccc tttagtcatt ggatgcgtac agacagtcag
     2761 tcggtatttc gtaattcaaa tgccagttac agtatgtcaa acgatttgaa aggcggcatg
     2821 accaacctat caggggttta tggcactctg ctgccggata ataacctgaa ttatagcgtt
     2881 caggtcggta acacccaagg gggtaataca tcgtctggca ccagtggtta cagttctctt
     2941 aattatcgtg gagcttatgg taatacgaat gtcggttaca gtcggagtgg tgacagcagc
     3001 cagatttatt acggaatgag tggtgggatt attgctcatg ctgatggcat cacctttgga
     3061 cagccgctgg gcgacacaat ggttctggtt aaggctcctg gcgctgataa tgtcaaaata
     3121 gagaaccaga ccggaattca taccgactgg cgtggctatg caatattacc atttgcgaca
     3181 gaatatagag aaaatcgtgt cgctcttaac gcgaattccc ttgcagataa tgttgaactg
     3241 gatgaaaccg tagtcactgt catcccaact cacggtgcta ttgccagagc aacatttaat
     3301 gcacaaatcg gcgggaaagt attaatgacg ttgaagtacg gtaataaaag cgttccattc
     3361 ggtgcaattg tcactcacgg agagaataaa aatggcagca ttgtcgcgga aaatggtcag
     3421 gtttatctga ctggacttcc acagtcaggg aaattacagg tttcatgggg caatgataaa
     3481 aactcaaact gtattgtcga ttacaagctt cctgaagtct ctcctggaac cttgctgaac
     3541 cagcagacag caatctgtcg ctaaggaaca atagattatg aaatacaata acgttatttt
     3601 cctcggttta tgtctggggt taaccaccta ttctgcttta tccgcagata gcgttattaa
     3661 aattagcggg cgcgtcctcg attatggctg cacagtctca tcggattcgc ttaattttac
     3721 cgtagatctc caaaaaaaca gtgccagaca atttccaacg accggtagca caagtccagc
     3781 cgtccctttt cagattacgt taagtgaatg cagcaaaggg acaacggggg ttcgggttgc
     3841 atttaatggt attgaggatg cagaaaataa tactctgttg aaactggatg agggaagcaa
     3901 tacggcctcc ggtttgggta tagaaatact ggacggaaat atgcgtccgg tgaaactgaa
     3961 tgaccttcat gccgggatgc agtggatccc actggtacca gaacagaaca atattttgcc
     4021 ttactccgct cgtctgaagt caactcagaa gtccgtcaat ccgggactgg tgagggcttc
     4081 ggcaaccttt acccttgaat ttcaataatc agagtcagat atgaaaagac ttcacaagag
     4141 gttcctgtta gctacgtttt gcgcgttatt cacagcaact ctccaggccg ccgatgtcac
     4201 tatcactgtt aatggtcggg tagtcgctaa accctgcact attcaaacca aagaagctaa
     4261 cgttaatctc ggggatcttt atacgcgcaa tctgcaacaa cctggttctg catctggctg
     4321 gcacaatatt actttgtcat taaccgattg tccggctgaa acaagtgcag tgacggcaat
     4381 cgtgacaggt tcaactgaca atacgggtta ttacaaaaat gaaggtactg ccgaaaatat
     4441 tcagatagag ctaagggatg accaggatgc gacgttaaaa aatggcgata gcaaaacggt
     4501 tatggttgat gagatcactc gtaatgcaca gtttccactt aaggcaagag ctatcacggt
     4561 gaatggaaac gcaagccagg gaacgatcga ggcgctaatc aatgtgatct acacctggca
     4621 ataacgtcga ttcgattttc ttagcct
//
LOCUS       DAZFFG010000222         4553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFFG010000222 DAZFFG010000000
VERSION     DAZFFG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.223"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..697
                     /locus_tag="ACHJ7J_004864"
     CDS             <1..697
                     /locus_tag="ACHJ7J_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5002963.1"
                     /translation="GANNRDVITRTFTVQLGATRVIYNPESSGATLTVTNQQNYPMLV
                     QSEVLSEDQKSRAPFIITPPLFRLDAQQSSKLRIVRTGGNFPEDRETLQWICVKGIPP
                     KNDDIWARDTSGKESSQDSISLQLQFSVNSCIKMFIRPSSLKGNPDDFANRIYWEKHG
                     DKLKGNNPTPFYMNISELSVGNKKISHRHYIPPFSSYEFDYPKSATGQIQWRVMTDYG
                     ISGKNNQAAIKNH"
     gene            725..3277
                     /locus_tag="ACHJ7J_004865"
     CDS             725..3277
                     /locus_tag="ACHJ7J_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5002964.1"
                     /translation="MRRKTFYTLIVISINGVFFPEAYATETTGYVYKFDASLIKNHGE
                     YTDLTIFEQGAQLPGIYPVDVLLNGKKVDSSELAFHIEKDPDGKPFLKTCLSREMLIR
                     YGVKVEDYPSLFPNNSESERVSCADLSAIPQSTETFQFSEQKLLLGIPQIALRPEWSG
                     IAPVALWDDGLSALLMNYQISANRTHYKGYGRFDSNALWSNLEPGLNIGPWRLRNMTT
                     WQKQSGQNGKWTSVYTRAERGLNSIKSRLTFGEFYTPSDIFDSVSLRGVMLSSDDSMV
                     PYNQRSFAPVVRGIAQTQSRVEVRQNGYLIYSTTVAPGEFALNDLPVTGSGGDLQVTV
                     HETNGTTQEFTVPFTTPAISLHQGYLKYSISGGQYRSPGYGRNHINVEQASVMYGLPW
                     NLTAYGGVQGADHYLAGALGIGVSMGSFGALSVDGTHTRGQQKGQDTRTGYSWRTRYS
                     KSYPLTGTNFTAANYQYSSEGYHTLSDVLNTYQDGNASTIPKNNRYQRTTLILSQSLG
                     KWGYLSLNSSQDKYRNSLPSQNSWGASYSTSWKGISWSVSWAENRVTRDYIRGKSQRE
                     NNFSLWINVPLKRWIGGNDNDIHATGQIQTGSGQGTQYEAGVNGRAFDRRLYWDIREQ
                     LTSGNTATGDNSNINLTWDGTYGEVTGNYSYNKNVSQMSAGISGGIVAYNGGVIFSQR
                     LGDTTAIIEAPGADGVSVNNWPGVRTDFRGYTTQGYLAPYNENIITLNPTTLPEDVEI
                     PQTDVRIVPTKGAVVPASFITRVGGRALINILRAEGKPAPFGAIVTLQGDAGKNNDAG
                     VVGENGEVYMSGLPAKGELLVQWGNNHICHATYRLPEKKGPAGVYLTQAVCK"
     gene            3300..3728
                     /gene="afaD"
                     /locus_tag="ACHJ7J_004866"
     CDS             3300..3728
                     /gene="afaD"
                     /locus_tag="ACHJ7J_004866"
                     /inference="COORDINATES: protein motif:HMM:NF017581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfaD family invasin"
                     /protein_id="HFO5002965.1"
                     /translation="MKKFTVTVLVMMFIVASSEDSYSAHLEINIRPVLNNIQHDGEHV
                     GNGQIVCSNDHSGFHIYGGYGTEDIYPWKYNAHGKSNPYNKLTVRLEGDNWIKNEQDY
                     PGIVKFSSEEAAEFRIVIDGKQKVPADEYTIRIFGECIIF"
     gene            3886..4404
                     /locus_tag="ACHJ7J_004867"
     CDS             3886..4404
                     /locus_tag="ACHJ7J_004867"
                     /inference="COORDINATES: protein motif:HMM:NF019295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002966.1"
                     /translation="MKKMIIMGAIALTVCSSGVYAIPLGNTANAEGTTYITQQVDTTI
                     TVEGKNLTAPVQQNTAVYSVNATTIGGADAILVWTDDNYIDSNGNMLARDDNGKEWLV
                     GFSDTDVTCNAGGTYSLVTSAEVGLSSKDNYIIKCTGNNNIASVNFDVRNQEQWPAGN
                     YTLKMQAVGWTN"
BASE COUNT         1464 a          846 c          987 g         1256 t
ORIGIN      
        1 aggtgcaaat aatagggacg taataacacg tacatttact gtacaactag gggctaccag
       61 agttatttat aatccagaat cttctggtgc aacattgact gtcactaatc agcaaaacta
      121 cccaatgctc gttcagtctg aagtcctttc tgaggaccag aaatcgcggg ctccctttat
      181 tattacccca cccttatttc gtcttgatgc acaacagtct agcaaattac gaattgttcg
      241 tactggtggt aacttcccgg aggatcgcga aacactacaa tggatctgtg taaaaggcat
      301 tccgcctaaa aatgacgaca tatgggcacg agacactagt ggaaaggaat caagccaaga
      361 ttctatatcc cttcagctac agttttctgt taacagttgt attaagatgt ttatccgtcc
      421 atcttcattg aagggtaatc ctgatgattt tgccaataga atttattggg aaaaacacgg
      481 tgataaattg aaagggaaca atccaacacc attttacatg aatatatctg aactaagtgt
      541 gggaaataaa aaaatttctc atagacatta catcccacct ttttcttctt acgaatttga
      601 ttatccaaaa tcagccactg gccaaattca atggcgcgtc atgactgatt acggaatatc
      661 gggaaaaaat aatcaggcgg ctattaaaaa tcactgatat ttatacaaat ataggtatat
      721 ccgtatgcgt agaaaaacgt tttatacttt aatagtcata tctataaacg gtgtattttt
      781 cccagaagcg tatgcaacgg aaacaacagg atatgtatat aaatttgatg catcattgat
      841 aaaaaatcat ggtgaatata ctgatctcac aatatttgaa caaggtgctc agttgcctgg
      901 tatttatcca gttgatgtac tcctcaacgg caagaaagtc gattccagtg aactggcttt
      961 tcatattgaa aaggatcctg acggaaaacc attcttgaaa acctgtctat ccagagaaat
     1021 gcttattcgc tatggagtta aggtggagga ttatccttca ctatttccaa ataattcaga
     1081 atcagagcgt gtttcttgtg ccgatttatc agcaatccca caatctactg aaacttttca
     1141 gttttcggaa caaaaactgc ttcttgggat tccccagata gccctacgtc ctgaatggag
     1201 tggtattgca cctgtagcct tgtgggatga cgggctgtca gcactactta tgaattatca
     1261 gatcagcgct aaccgtacac attataaggg atatggaaga tttgatagta atgcgctatg
     1321 gagtaacctt gaaccaggac tgaatatagg tccttggcgt cttcgaaata tgaccacctg
     1381 gcagaaacaa tcaggtcaga acggaaaatg gacatcagta tataccagag cagaaagagg
     1441 actgaattcc atcaaaagtc gtctgacatt tggcgaattt tatacacctt ctgatatttt
     1501 cgacagcgta tccttacgcg gggttatgct aagttccgat gatagtatgg taccttacaa
     1561 ccagcgttcc tttgcaccag tagttcgggg aattgctcag actcaatccc gagttgaagt
     1621 tcgccagaat ggttatctaa tttacagtac tactgttgct ccgggagaat ttgccctaaa
     1681 tgatctacct gtgactggat ctggtggcga tctgcaggtt acagtccacg agaccaacgg
     1741 aaccacccaa gagttcacag taccttttac tactccagct atttcgcttc accaaggata
     1801 tttaaaatat agcatatccg gcgggcaata ccgttctcct ggatatggaa ggaatcatat
     1861 caatgtagag caagcaagtg tcatgtacgg ccttccatgg aatttgactg catatggtgg
     1921 agtacaagga gcagatcatt acctagcagg ggctctggga ataggtgtgt ctatgggatc
     1981 atttggagca ctttctgttg acggaaccca tacccgaggc caacaaaaag gtcaagatac
     2041 aagaacggga tactcttggc gtacccggta tagcaaatca taccctctaa caggaaccaa
     2101 ctttactgct gcaaactatc aatattcttc ggaaggttac catacgctat ctgatgtcct
     2161 taatacatat caagatggga atgcatcaac tataccgaaa aataataggt accaacgaac
     2221 cacactaatt ctgagccaat ctttaggtaa atggggatac ctatcattga atagctctca
     2281 ggataaatac cgtaatagtt tgccatccca gaattcttgg ggagcttctt atagtacttc
     2341 ctggaaagga atatcttggt cagtaagttg ggcggaaaat cgtgtaacac gtgattacat
     2401 ccgtggtaag agtcagcgtg agaacaactt tagtttatgg ataaacgtgc cattgaaacg
     2461 ctggataggc ggaaatgata atgatattca tgccactggt cagatccaga ctggttctgg
     2521 gcaaggtacc caatatgagg ctggagtgaa cggtcgggct tttgatcgac gcctgtactg
     2581 ggacatcagg gaacaactaa cttctgggaa tacagctaca ggagataata gcaatataaa
     2641 cctgacatgg gatggaacat acggtgaagt cactggtaac tatagttaca acaaaaatgt
     2701 cagtcaaatg agtgcaggaa tatccggagg aattgtcgcc tacaacggtg gtgtcatttt
     2761 cagccaacgt ttgggagata ctacagcgat aattgaagca ccaggtgctg atggtgtatc
     2821 agtcaacaac tggccaggtg taagaacaga ttttcgagga tataccaccc aaggatatct
     2881 tgctccttat aatgaaaaca ttatcacact caacccgaca acacttccgg aagatgttga
     2941 aattcctcaa acagatgtcc gtattgtgcc aacaaaaggg gcagttgtgc cagccagttt
     3001 tatcaccaga gtaggtggcc gggcactcat taatatactt cgtgcagaag gaaaaccagc
     3061 accttttggt gcaattgtaa ccctgcaagg tgatgccggg aaaaataatg atgctggtgt
     3121 ggtaggggag aatggtgaag tatacatgag tggcctgcca gcaaagggag aactgcttgt
     3181 tcagtggggt aataaccata tttgccatgc cacttaccga ttacctgaaa agaaaggacc
     3241 tgcaggtgtc tatttaacac aggctgtctg taagtaaacg gtgagaacag gagacagtaa
     3301 tgaagaagtt tacagtaact gttttggtga tgatgttcat tgttgctagt tcagaagaca
     3361 gttatagcgc acatttggaa attaatatca gaccagtttt gaataatatt cagcatgatg
     3421 gtgaacatgt tggtaatggg cagatcgtat gcagtaatga ccacagcggg ttccacatct
     3481 atggaggata tggaacagaa gatatttatc cttggaaata taatgcacat ggaaagagta
     3541 atccatataa taaattaaca gtgcgtctgg aaggtgataa ctggattaaa aatgaacagg
     3601 actatcctgg aatagtgaag tttagtagtg aagaagcagc tgaatttcgt attgttatag
     3661 atggaaaaca aaaggttccg gctgacgaat atactatccg aatattcgga gaatgtatta
     3721 ttttctagct tatcatacag aaaagtattt tccgaaatgt ttacaatgta aaaattacat
     3781 tggttacatc ggaaaacagt cagatattat gtatctgact gtactgatat gttttatata
     3841 taaaacatat tatgttgtca ataaatagta taatgagagt atatcatgaa aaaaatgata
     3901 atcatgggag ctattgctct gactgtatgt tcatcaggag tatatgctat acctttaggt
     3961 aatacagcta atgcagaagg tactacatat atcacacagc aggttgatac tactattaca
     4021 gtagaaggta aaaatctcac tgctcctgta cagcagaata ccgctgtgta ttcagtgaat
     4081 gccaccacta ttggtggtgc ggatgcaatt cttgtatgga cggatgataa ctacatagac
     4141 agcaatggta atatgttagc aagggatgac aatggaaaag aatggctagt tggtttttcg
     4201 gatactgatg ttacatgcaa tgccggggga acttattcac tggtaacttc tgctgaagta
     4261 ggacttagta gtaaagataa ttatattatc aaatgcacag gaaataataa tatagcatcc
     4321 gtaaattttg atgtaagaaa tcaagagcaa tggcctgctg gaaattacac actgaaaatg
     4381 caggcagtag gctggacaaa ttaatttaat acggagaaca gtaaaatatg aaaaaaatag
     4441 catatacatt aggtgctctt attttttctt ccagctccat ggctggaatt gtaggtaata
     4501 tcgcaacatc agagggtacc acatatgttc aggatactgt gaataccact cta
//
LOCUS       DAZFFG010000223         4503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFFG010000223 DAZFFG010000000
VERSION     DAZFFG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.224"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            138..680
                     /gene="fimA"
                     /locus_tag="ACHJ7J_004868"
     CDS             138..680
                     /gene="fimA"
                     /locus_tag="ACHJ7J_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5002967.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            900..1592
                     /gene="fimC"
                     /locus_tag="ACHJ7J_004869"
     CDS             900..1592
                     /gene="fimC"
                     /locus_tag="ACHJ7J_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5002968.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1623..4232
                     /locus_tag="ACHJ7J_004870"
     CDS             1623..4232
                     /locus_tag="ACHJ7J_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO5002969.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCIDLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4245..>4503
                     /gene="fimH"
                     /locus_tag="ACHJ7J_004871"
     CDS             4245..>4503
                     /gene="fimH"
                     /locus_tag="ACHJ7J_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="involved in the regulation of fimbriae length and
                     mediates adhesion of type 1 fimbriae; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HFO5002970.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNF"
BASE COUNT         1218 a          965 c         1060 g         1260 t
ORIGIN      
        1 ttttttagaa tagagtgcat attttcaatt aagacattct tagaggataa aaaggaattt
       61 actactatca gtgtcttaaa taaagtaatc ggttatatac ggatgtggag tcgataaatg
      121 agattgaagg aatatatatg aaattaagat ttatttcgtc tgcgctggct gccgcactat
      181 tcgccgctac gggtagttat gctgccgttg tagatggcgg tacaattcac tttgaaggcg
      241 aactggtgaa tgctgcctgt tcagtgaata ctgactcggc agaccaggtt gtcacactcg
      301 gtcaatatcg taccgatatt ttcaatgctg ttggtaatac ctctgcatta attccattca
      361 ctatacagtt gaacgactgc gatcctgttg ttgccgctaa tgctgccgtt gcattttctg
      421 gtcaggctga tgcaatcaat gataatttat tggccattgc atccagtacc aatacaacaa
      481 cagcaacggg tgtcggtatt gaaatacttg ataatacatc cgcaattctc aaacctgatg
      541 ggaatagctt ctcaaccaac cagaacttga tccccgggac caacgttctt catttttctg
      601 cacgttataa aggcaccggt acaagtgcat cagcagggca agcaaatgct gacgcgactt
      661 ttattatgag atacgaataa tcaaaaccac gttgttttga attatatatc acgtcttata
      721 acaaagtaat gtaccggttg tctgaagcgg tatggtggca atgtaaatcg aaatcatgtt
      781 cactttgtat catgccgctt tattaaatga aaagggaatg atgtgttgta agaaaccaaa
      841 gcaatcattt ctttatattt cttatttttg ccgtcaggaa tacacaaggc gtattaacta
      901 tgatgactaa aataaagtta ttgatgctca ttatatttta tttaatcatt tcggccagcg
      961 cccatgctgc cggagggatc gcattaggtg ccacgcgtat tatttatccc gctgatgcta
     1021 aacagactgc ggtatggatt agaaatagcc ataccaatga gcgttttctg gtcaattcgt
     1081 ggattgaaaa cagcagcggt gtaaaagaaa agtcattcat cattacaccg ccactatttg
     1141 ttagtgaacc caaaagcgaa aatactttgc gtattattta caccggtcca ccgctggcgg
     1201 cagatcgtga gtctctgttc tggatgaatg ttaagacgat cccttcggta gataaaaatg
     1261 cattgaacgg caggaatgtt ttgcaactgg cgattttatc gcgcatgaag ttatttctcc
     1321 gtccaattca attacaagaa ttacccgcag aagcgccgga cacactcaag ttttcgcgat
     1381 ccggtaacta tatcaatgtt cataatccat caccttttta tgtcaccctg gttaacttac
     1441 aagtgggcag ccaaaagttg gggaatgcta tggctgcacc cagagttaat tcacaaattc
     1501 ccttaccctc aggagtgcag ggaaagctga aatttcagac cgttaatgat tatggttcag
     1561 taaccccggt cagagaagtg aacttaaact aaccgaatca tctgacaata tcagagctaa
     1621 ttatgaaaat acccactact acggatattc cgcagaggta tacctggtgt ctggccggaa
     1681 tttgttattc atctcttgcc attttaccct cctttttaag ctatgcggaa agttatttca
     1741 acccggcatt tttattagag aatggcacat ccgttgctga tttatcgcgc tttgagagag
     1801 gtaatcatca acctgcgggc gtgtatcggg tggatctctg gcgtaatgat gagttcattg
     1861 gttcacagga tatcgtattt gaatcgacaa cagaaaatac aggtgataaa tcaggtgggt
     1921 taatgccctg ttttaaccag gtactccttg aacgaattgg ccttaatagc agtgcatttc
     1981 ccgagttagc ccagcagcaa aataataaat gtatcgattt actgaaagct gtacctgatg
     2041 ccacaattaa ctttgatttt gcagcgatgc gcctgaacat cactattcca cagatagcgt
     2101 tgctgagtag cgctcacggt tacattccgc ctgaagagtg ggacgagggt attcctgctt
     2161 tactcctgaa ttataatttc accggtaaca gaggtaatgg taacgatagc tattttttta
     2221 gtgagctcag cgggattaat attggcccgt ggcgtttacg caacaatggt tcctggaact
     2281 attttcgcgg aaatggatat cattcagaac agtggaataa tattggcacc tgggtacagc
     2341 gcgccattat tccgctgaaa agtgaactgg taatgggaga cggcaataca ggaagtgata
     2401 ttttcgatgg tgttggattt cgtggtgtac ggctttattc ttccgataat atgtatcctg
     2461 atagccagca agggtttgcc ccaacagtac gtgggattgc ccgtacggcg gcccagctaa
     2521 cgattcggca aaatggtttt attatctatc aaagctatgt ttcccccggc gcttttgaaa
     2581 ttacagattt gcacccgaca tcttcaaatg gcgatctgga cgtcaccatc gacgagcgcg
     2641 atggtaatca gcagaattac acaattccgt attcaacagt gccaatttta caacgcgaag
     2701 ggcgtttcaa atttgacctg acggcgggcg attttcgtag cggtaatagt cagcaatcat
     2761 cgcctttctt ttttcagggc acggcactcg gcggtttacc acaggaattt actgcctacg
     2821 gcgggacgca attatctgcc aattacaccg cctttttatt agggctgggg cgcaacctcg
     2881 ggaactgggg cgcagtgtcg ctggatgtga cccatgcgcg cagccagtta gccgacgaca
     2941 gtcgtcatga gggggattcc attcgcttcc tctatgcgaa atcgatgaac accttcggca
     3001 ccaattttca gttaatgggt taccgctatt cgacacaagg tttttatacc cttgatgatg
     3061 ttgcgtatcg tcgaatggag gggtacgaat atgattacga ttacgacggt gagcatcgcg
     3121 atgaaccgat aatcgtgaat taccacaatt tacgctttag ccgtaaagac cgtttgcagt
     3181 taaatatttc acaatcactt aatgactttg gctcgcttta tatctctggt acccatcaaa
     3241 aatactggaa tacttcggat tcagatacct ggtatcaggt ggggtatacc agcagctggg
     3301 ttggcatcag ttattcactc tcattttcgt ggaatgaatc tgtagggatc cccgataacg
     3361 aacgtattgt cggacttaat gtttcagtgc ctttcaatgt tctgaccaaa cgtcgctaca
     3421 cccgggaaaa tgcgctcgac cgcgcttatg cctcctttaa cgccaaccgt aacagcaacg
     3481 ggcaaaatag ctggctggca ggtgtaggtg ggaccttact ggaaggccac aacctgagtt
     3541 atcacgtaag ccagggtgat acctcgaata atgggtatac gggcagcgcc acggcaaact
     3601 ggcaggccgc ttacggtacg ctgggggtcg ggtataacta cgaccgcgat caacatgacg
     3661 ttaactggca gctgtctggc ggagtggtcg ggcatgaaaa tggcataacg ctgagccagc
     3721 ctttagggga taccaatgtt ttgattaaag cgcctggcgc aggcggtgta cgcattgaaa
     3781 atcaaactgg cattttaacc gactggcgcg gctatgcggt gatgccgtat gccacggttt
     3841 atcggtataa ccgtatcgcg cttgatacca atacgatggg gaattccatc gatgttgaaa
     3901 aaaatattag cagcgttgtg ccgacgcaag gcgcgttggt tcgtgccaat tttgataccc
     3961 gcataggcgt gcgggcgctc attaccgtta cccagggcgg aaaaccggtg ccgtttggat
     4021 cactggtacg ggaaaacagt accggaataa ccagtatggt gggtgatgac gggcaagttt
     4081 atttaagtgg tgcgccattg tctggtgaat tactggttca gtggggagac ggcgcgaact
     4141 cacgctgcat tgcgcactat gtattgccga agcaaagctt acagcaagcc gtcacagtta
     4201 tttcggcagt ttgcacacat cctggctcat aaaggaaatt atcaatgaag ataatctgca
     4261 gattattatt ggcaatggca tgtttgtgtc tggcaaacat atcctgggct actgtttgtg
     4321 caaatagtac tggcgtagca gaagatgaac actatgatct ctcaaatgtc tttaatagca
     4381 ccaataacca gccagggcag attgttgttt taccggaaaa atccggctgg gtaggtgtgt
     4441 cagcaatttg cccacccggc acgctggtga attatacata ccgtagttat gtcaccaact
     4501 tta
//
LOCUS       DAZFFG010000224         4377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFFG010000224 DAZFFG010000000
VERSION     DAZFFG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.225"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            89..1036
                     /gene="stxA1d"
                     /locus_tag="ACHJ7J_004872"
     CDS             89..1036
                     /gene="stxA1d"
                     /locus_tag="ACHJ7J_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1d subunit A"
                     /protein_id="HFO5002971.1"
                     /translation="MKIMIFRALTFFFVIFSVNAIAKEFTLDFSTAKKYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDIMGLEPEEERFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTRAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSYSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSILPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMTP
                     DEFPSMCPTDGSGRGITHNKILWDSSTLGAILIRRTISS"
     gene            1046..1315
                     /gene="stxB1d"
                     /locus_tag="ACHJ7J_004873"
     CDS             1046..1315
                     /gene="stxB1d"
                     /locus_tag="ACHJ7J_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1d subunit B"
                     /protein_id="HFO5002972.1"
                     /translation="MKKVLLIAVSLSFLSASVLAAPDCVTGKVEYTKYNDDDTFTVKV
                     ADKELFTNRWNLQSLLLSAQITGMTVTIKTTACHNGGGFSEVIFR"
     gene            1672..1899
                     /locus_tag="ACHJ7J_004874"
     CDS             1672..1899
                     /locus_tag="ACHJ7J_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5002973.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LALGFLTYLTNLYFKIKEDRRKAARGESFND"
     gene            1892..2206
                     /locus_tag="ACHJ7J_004875"
     CDS             1892..2206
                     /locus_tag="ACHJ7J_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO5002974.1"
                     /translation="MIKSYELIVKGPRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            2262..2795
                     /locus_tag="ACHJ7J_004876"
     CDS             2262..2795
                     /locus_tag="ACHJ7J_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5002975.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDNALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRISSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            3012..3197
                     /locus_tag="ACHJ7J_004877"
     CDS             3012..3197
                     /locus_tag="ACHJ7J_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5002976.1"
                     /translation="MRKLKMKLCALMLPLVVSACGSMPVAPQPCVKPPDPPAWIMQPA
                     PDWQTPLNGIISPSENG"
     gene            3302..3652
                     /locus_tag="ACHJ7J_004878"
     CDS             3302..3652
                     /locus_tag="ACHJ7J_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5002977.1"
                     /translation="MPSRIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDDDSNLESLC
                     LECHRAKTARERIR"
     gene            complement(3788..4303)
                     /locus_tag="ACHJ7J_004879"
     CDS             complement(3788..4303)
                     /locus_tag="ACHJ7J_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003841965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002978.1"
                     /translation="MDKTRKYDRALQLEILNALIDCAPHSLNKAQERDLIEKFDSYDH
                     FVACMLYLEMHGLVSTPFLRSDTMAGVDFIFNAAYCNITEKGIDFLLDDGGLSAILKV
                     QTVRLHSDTIVALEDIIRVSNISEDQKKGLISKLRELPGDAIKHLTLQLLTQGVLNLP
                     NALRLIQTTLQ"
BASE COUNT         1183 a          867 c         1122 g         1205 t
ORIGIN      
        1 aacccatgat aatataaatt tatgattcat gtagcagcat ttattatgaa tatagttatc
       61 gtatggtgct caaggagtat tgtgtaatat gaaaataatg atttttaggg cgctaacatt
      121 tttcttcgtt atcttttcag ttaatgcgat tgctaaggag tttaccttag atttctcaac
      181 agcaaagaag tatgttgatt cgctgaatgt cattcgctct gcaataggta cgccattaca
      241 gactatttca tcgggaggta catctttact gatgattgat agtggcacag gcgataattt
      301 atttgcagtc gatatcatgg ggttagaacc agaggaagag cggtttaaca atctacgact
      361 gattgttgaa cgaaataatt tatatgtgac aggatttgtt aacaggacaa acaatgtttt
      421 ttatcgcttt gctgattttt cacacgttac ctttcctggt acaagagcgg ttacattgtc
      481 tggtgacagt agctatacca cgttacagcg tgttgcgggg atcagtcgta cggggatgca
      541 gataaatcgc cattcgctga ctacttctta tctggatttg atgtcgtata gcggaacctc
      601 actgacgcag tctgtagcaa gagcaatgtt acggtttgtt actgtgacag ctgaagcttt
      661 gcgttttcgc caaatccaga ggggatttcg tacaacactt gatgatctca gtggacgttc
      721 ttatgtaatg actgctgaag atgttgatct tacattgaat tggggaagac tgagtagtat
      781 tctgcccgac tatcatggac aagactccgt tcgtgttgga agaatttctt ttgggagtat
      841 taatgcaatt ctgggaagcg tggcattaat attaaattgc catcatcatg catcacgagt
      901 tgccagaatg acacctgatg agtttccttc tatgtgcccg acagatggaa gtgggcgtgg
      961 gattactcac aataaaatat tgtgggactc atctacactg ggggcaattt tgattcgcag
     1021 aactatcagt agttgagggg gtaaaatgaa aaaagtatta ttaatagctg tttcactttc
     1081 atttctttca gcaagtgttc tggcagcgcc agattgtgta accggaaagg tggagtatac
     1141 aaaatataat gatgacgata cttttacagt taaagtggca gataaagaat tatttactaa
     1201 cagatggaat cttcagtctc ttcttctaag tgcacaaatt acggggatga cggtaactat
     1261 taagactact gcttgtcata atggaggggg attcagcgag gttatttttc gttgattcag
     1321 aatagccctg tgaagagcac aggaggaggt ttatcaatgt taaatatgtc aattcagtca
     1381 gttgacagtt gcctgactgg cagagctttt gtaaaaaaat cttgcatagg gaatcccgca
     1441 gtgcagagtg gaaactggtg gacgatatca tctctgatca tcgttaacga taatgaggcg
     1501 ggttcagtgg tgcagggcta aattcatcgg gaggaactag gcaacatgca tcaatgaaaa
     1561 tattgttaat tgagtgtttt tcatacatga ctggtaaagg tgattcaggc catcagagtt
     1621 ttgcggatgg ccttttttct ttccggtagc acagatctgt cttggttgga tatgtatcag
     1681 atggaaaaaa tatcaacagg aattgcctac ggcacctccg caggcagtgc tggctactgg
     1741 tttttacagt ggcttgatca ggtcagtccg tcacagtggg cggcaattgg cgtactggga
     1801 agtctggcac tgggctttct gacttatctg acgaatctgt acttcaagat aaaagaggac
     1861 cgtcgcaagg cggcgagggg agagtcattc aatgattaaa agctatgagt tgattgtgaa
     1921 agggccccgc aattttgaga ataaagttac ggtaacttta gcgttacggg acaaaaaacg
     1981 ctttgacggt gaaatttttg acctggacat ctcgctggac cgtgttgaag gtgccgcgct
     2041 ggagttttat gaggcagcag ccagaaggag catcagacag gtcttcctgg atgttgctgc
     2101 cgggttatgt gaaggggacg aactgttgcc agaaacgcgc ccctgttcag aggcgcggta
     2161 taccataaaa attaacagtt ctgataactc gattacgggt tgttagcttt ttgcagttgg
     2221 ctttccagta tctttcattg gtagcatcct gataaatatc catgagcgca aaaatcaaat
     2281 acggcctgtc agctgccgtt ctggcgctga ttgctgcagg cgcgtctgct cctcaaatac
     2341 ttgaccagtt tctggatgaa aaagagggta accacactac ggcataccgc gatggttccg
     2401 gtatatggac catctgtcgt ggtgccacaa tggtggatgg taagcccgtc ataccgggaa
     2461 tgaagctgtc gaaggaaaaa tgcgaccagg ttaacgctat tgaacgtgat aatgcgctgg
     2521 catgggtgga gcgcaatatt aaagtaccac tgaccgaacc acagaaagcg ggtatagcgt
     2581 cattttgtcc ctataacatt ggccccggta agtgtttccc gtcgacgttt tataagcggc
     2641 tgaatgccgg tgatcgtaag ggggcatgcg aggcgattcg ctggtggata aaagatggtg
     2701 ggcgcgattg ccgcataagt tcaaataact gctatggaca ggttattcgt cgtgaccagg
     2761 aaagcgcatt agcctgttgg gggatagatc agtgagcaga gtcgccgcga ttatttatgc
     2821 tctggttatc tgcatcatcg tctgcctgtc gtgggcggtc aatcattacc gtgataacgc
     2881 catcgcctac aaagaacagc gtgataaaaa agtcagtgag ctgaagcagg tgatcgccac
     2941 catcgctgac atgcagcagc gtcagcgtga tgttgctgcg ctcgatgcaa agtactcgag
     3001 agaattagcc aatgcgaaag ctgaaaatga aactctgcgc gctgatgttg ccgctggtcg
     3061 taagcgcctg cgggtcaatg ccagttgctc cgcagccgtg cgtgaagcca ccggatccac
     3121 cagcgtggat aatgcaacca gcccccgact ggcagacacc gctgaacggg attatttcac
     3181 cctcagagaa cggttgatga cgatgcagaa gcaactggaa ggggcgcagg aatatatccg
     3241 cacccagtgc attaagtagc ctttttatcg tggtaaacat ttcgcagggt atgaggtatt
     3301 tatgccatca cgaattccac gagcctgccg taagcgtgga tgcgcaggta caaccacaga
     3361 cagttctggt tactgcgata aacatcgtgg cgaaggatgg gtacagcatc aacgcggact
     3421 gagtcgccac cagcgtggct atggctcgaa atgggatgcc atacgtgcgc gcatactgaa
     3481 gcgtgataat catctgtgtc agaactgcct gcgcaatggg agagccgttg aggccaggac
     3541 tgtagaccac atcattccaa aagctcatgg cggcaccgat gatgactcca atcttgagtc
     3601 gttatgcctg gaatgccaca gagcgaagac agcaagggaa cgtattcgat gataatattc
     3661 actgctgtaa tgcataaaaa tagtttctat attcatgcag atacccggga gggattttgg
     3721 gttgttttaa gtgaaaaact gggatggggc aaatttgagt taattcgccc ctcagatgag
     3781 tttagcccta ctggagggtt gtttgaatta gtcgaagtgc gttcggcaga ttcagaaccc
     3841 cctgagtcag taactgtagg gtcaaatgtt ttatggcgtc tcccggaagc tctcggagtt
     3901 ttgaaatcaa tcccttcttc tgatcttcag atatatttga gacacggatt atatcctcaa
     3961 gggcaacaat cgtgtcactg tgtaaccgaa cggtttgaac tttaaggatt gcacttaagc
     4021 cgccatcatc gagaagaaaa tcaattcctt tctctgtaat gttgcagtac gcggcattga
     4081 agataaaatc aacgccagcc atggtatcgc tgcgtaagaa gggagtagaa acgagaccat
     4141 gcatttcaag gtataacatg cacgccacaa agtgatcgta actgtcaaac ttctcaatga
     4201 ggtctcgctc ctgtgccttg tttaaagagt gaggcgcgca atctataagg gcattgagga
     4261 tttcaagttg taaggctctg tcatattttc tggttttatc catttcttag actccatctt
     4321 ttatttattg agattaactg aatacaccat gctgagcatc ctgatggatg gccagta
//
LOCUS       DAZFFG010000225         4326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFFG010000225 DAZFFG010000000
VERSION     DAZFFG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.226"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(1..4169)
                     /locus_tag="ACHJ7J_004880"
                     /pseudo
     CDS             complement(1..4169)
                     /locus_tag="ACHJ7J_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
BASE COUNT          736 a         1425 c         1157 g         1008 t
ORIGIN      
        1 tatcgttttt ttatgctctg gattaatata atcaatacat ctatttgagc aatcttgtgt
       61 taatggacat gtcaacccat gttcatttac agccaagtct tttttattat cttcaatcat
      121 ttcagaatgg gatagcttta cttttctgcc gtacataccg aacagattta acccgtaatc
      181 tctaatctct ttttcataac ccagcccttt cgccgatcgg ctaacaccag cgtcatttaa
      241 tttagacact cgacaaaatg ccatacaatg gaaaaattga tcagtcccac ctaaatttaa
      301 tctcttcata tcttcataag tatcagataa tatatcccat gctctttgat ggtttagttg
      361 atcttttctt cttattagcg ctacatctgc gggacttaac cctaatggat caataccatt
      421 caccggattc agcggatacg catacagact ccatcccccc tccagcccta tcgggtcctg
      481 agtgatatac cgcctctgca acggatcgta gtaccggttc cggttgtagt acagccccga
      541 ctccttatca tactgctgcc ccggcagccg gtacggctgg tgcaggtgat gcgggttctc
      601 ctcattaagc tggttgcccc attcatcata ttccgcgctc cacgccgtat tgccgtcttc
      661 actgataagc gccagcggca ggccccggtg gtcgcagtgg taaagatgaa cttttctcgc
      721 cggtgtgtat tccggctcca cctgtctggc cagttgctcc accgtcagcc cgcactgcgc
      781 aagccacgcc cggctttcgt tactcacgcg gtctgcccgg atttcttcct ccagcctgtc
      841 cagcagccgc accagttcag ccgggaacac cacgccgtgg ccgttctcac tcccttcctg
      901 ctggagcatc tctgccaggc tgcgccgctg cgctttttcc cgctcgccgt tctctgtctc
      961 gatgcggatg agcggtgaga agctccccgg ctcgtatacc gtctggatac gcgtggtgtc
     1021 ggtctgtatc gtcgtcagcc tgtcaccgtc ccagccgtac cacgtctcct ccggtttacg
     1081 cgacagcgac atccatcctg tcaggtcacg cccgcgacgc cacacccgtt tacccgtccg
     1141 gcggcccagc gggtcgtaga ggtagcggct ctcgaccagt ggctcgccat gctgtatccg
     1201 cgtgtagaac accagacggt gctggctgtc gtagtggtag tggtgggtcc gctcgtcgtc
     1261 cgtccgtatc acacccgtcg ggatgcggtc cgtcttctcc gtcagcctgc cgtattcatc
     1321 gtaacggtac aggtagtgcg cgtcttcggt gattcggtta tccggccaca ctgtgagtgt
     1381 actgtccggg tgcagctccg ggtccggcag ccggtttccc gccgggtcgg tcgcatacgg
     1441 gatgcggatg tccaggtccg gtgcgagggt gcgcacgctc tccagcctgc ccgtggcgct
     1501 gtagccgtat tcccgcgtct gccggggggc gctgatgcgc accaggtcgc cgttgtcact
     1561 ccacccgtag tcacggtcat ataccaggct gttcaggtgc tggctctgta actggcctgc
     1621 gggggtgtat gtgctggtca gttcgtatgc ggcattactg cctgccatgc tgccgaagct
     1681 gcgcaccgtc tcacggtgca gcctgtcccg cgtatactcg accagcggcg tcccgcccag
     1741 cttcatgccc gccaggtaac cgctaccata cgtcagccac tccaccggcg gcaggctgtc
     1801 cggcgtgacg cggtttgcca gcccctgttc gttgtatgcg tgctctgtct catgctgcca
     1861 cagcagttcc cccgtctccg ggttatgcac cgtctggcgc tccccggcca ggcgccctta
     1921 tcatcataac cgtaatgcac cgccacctga tggccttcgc tcaggtggct gatgtctgtc
     1981 agccagccgt ggccgtcata ctgccactgc tctgccggtt caccgttcac cgtgcggtga
     2041 gtgatacggt ccgattcgtc gtagtgccag agggtgacaa gtccctcatc ctcactctgt
     2101 gtgagttttc cggtcaggtc ataatgataa cgctgcgtcc gcccgtcaaa gccgccctgc
     2161 tgtaccaggc ggtccagcgc atcgtagctg aagtcgctgt gactgccgtt ctcgttggtc
     2221 aggctgatga cacgtccggc agcatcgtac tccatactgc gcgtcagccc gccctgcgtg
     2281 gtgctgaccg cctttcccca cgcatcgtac tgtgtctcgc tccggttgcc gtccggggtg
     2341 ataacggcag tcaggtcgcc tgcggcgttg tattcatacc gcgtttcacg gccctgtgcg
     2401 tctttcaccg aggttaaccg gccacggttg tcatagcggc ggtaaaggct gatgccttcc
     2461 tcgcggtgga ccgccgtcat ctggccgaag cggtcgtatt catagcgggt ctggtagccc
     2521 gagcagtcgg tgaacgccag caactgcccg tagcggctcc acgtcatctg ccgggtgctg
     2581 cccgtcgcat ctgttgttgt ggccggtaat tcactgtgcg catcatcata gcggtagcgt
     2641 actgtctccc cgctgcgcga tgtctccgtt accagcctgt cccgctcatc atacgcccgg
     2701 cggctctcca gcccgtccgg gtacaccacc gccgtcacca ggttcccgtc gttatagtaa
     2761 aatttcgtct cccgcccgtc cggtgtggtg atgtccgtga tgtcgcctga caccacattc
     2821 agaccgtact ctgtccgccg tcccgccgcg tccgtctgcg ccgtgagcct tcctgccgcg
     2881 tcatacccgc tgtgcgtgac gctgccgtcc gccagttctt ttttcaccac ccgcttcagc
     2941 ccggccccgc cttctgtatg cagcacctca cgccggttca ggctgtctgt gacggtgatg
     3001 cggtcctgct catactggta gcagtaactc aggcctgccg ggtttagttg ctccaccacc
     3061 cgccccgcat cgtcgtagcg gtagcgcatc tccggccttc ccgcgtaacg gtgtgccacc
     3121 atccgcccgg ggtgctgcgc gtcatatgtg aaagcgcgca cctgcgtatt gctgcggtca
     3181 tataccgcca gcagttcacc ggcctccgta tacgtgtacc gcaccagggg cgcaccgggc
     3241 aggctctccg ggtacgccgg gtcgtgcatc agccacaccg ccgaaaggcg gatacccctg
     3301 tcggggccgt attcggtacc gggcagtgtg tcggggaacg ctgaggctga gagagggcgg
     3361 gaacggtcag aagaagatag cgaagaggtg cgggcctctt ccgcacgctg cacctgcgtg
     3421 gtcagcacca gacggaactc ccgcccggca ccgtccgtca cgccggtgat ttccccggcc
     3481 aggtcgcctg cggcctcacg ccggtacgtc agcgtccgcc cgaagcggtc cgccatcccg
     3541 gtaagcaccc ggtacggcgg cagcggcgct ggcagtacgt cctcagcacc cggcacccgc
     3601 tcagaccacc ccagtatcca ccacggcccc tgtgcgctgt tggtcgccag gtaaagatgc
     3661 gggcttaacc ggatatccgg cggcagcgac gcccacagcc gtgccagcgt gtggccgtcc
     3721 ggctgtgctg ccttaccacc gcgcaccagc cacattgact cgctgcggct gtacaccgcc
     3781 tcacccggca gcagcggctc aaagtgaatg ctccgtccgc cgttgtcgtt gagtaccagt
     3841 gcatcatcgc gtagctgtaa gcggatatca gaaggcgctt tccagccggg gccaaaaatc
     3901 cccaccggtg caggcgtctt cgtccggtag ctgctgtagg tgcgggagag aatgaacggc
     3961 agcgggccgg gcagcgcaag gtccgtctcg ccgggcagca ccttcgcccc cagcagcgga
     4021 tttaccgggt tgcccgaagt catcccgccc ggacacaccg agcacgccac gccagttggc
     4081 gcgccaattc ttacacctgc cgaaccctgg acaatgggac cgccatactg agtcatatct
     4141 ccctgacgcg ccgctggttt tccgctcata taaagtgccc ttttagtttt gttcatatcc
     4201 ctgtgttagc aacaaaaaaa ataaaatgac acacaagacc agctaatgta tttatatatt
     4261 tttcacataa acttatttgt tctcaatcaa caacaactgt tattacaggt cactttcaat
     4321 atttta
//
LOCUS       DAZFFG010000226         4305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFFG010000226 DAZFFG010000000
VERSION     DAZFFG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.227"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..290)
                     /locus_tag="ACHJ7J_004881"
     CDS             complement(<1..290)
                     /locus_tag="ACHJ7J_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5002979.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAA"
     gene            complement(405..480)
                     /locus_tag="ACHJ7J_004882"
     tRNA            complement(405..480)
                     /locus_tag="ACHJ7J_004882"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Thr,seq:ggt)
     gene            complement(487..561)
                     /locus_tag="ACHJ7J_004883"
     tRNA            complement(487..561)
                     /locus_tag="ACHJ7J_004883"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(526..528),aa:Gly,seq:tcc)
     gene            complement(678..762)
                     /locus_tag="ACHJ7J_004884"
     tRNA            complement(678..762)
                     /locus_tag="ACHJ7J_004884"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(726..728),aa:Tyr,seq:gta)
     gene            complement(771..846)
                     /locus_tag="ACHJ7J_004885"
     tRNA            complement(771..846)
                     /locus_tag="ACHJ7J_004885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(811..813),aa:Thr,seq:tgt)
     gene            1208..2158
                     /gene="coaA"
                     /locus_tag="ACHJ7J_004886"
     CDS             1208..2158
                     /gene="coaA"
                     /locus_tag="ACHJ7J_004886"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO5002980.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2187..3152)
                     /gene="birA"
                     /locus_tag="ACHJ7J_004887"
     CDS             complement(2187..3152)
                     /gene="birA"
                     /locus_tag="ACHJ7J_004887"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO5002981.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3149..4177)
                     /gene="murB"
                     /locus_tag="ACHJ7J_004888"
     CDS             complement(3149..4177)
                     /gene="murB"
                     /locus_tag="ACHJ7J_004888"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO5002982.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGRVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1093 a         1068 c          976 g         1168 t
ORIGIN      
        1 gcagcaccgg tgatcatgtt tttaacatag tcggcgtgcc ccgggcagtc tacgtgtgcg
       61 tagtgacggg tcggggtgtc gtattcaacg tgagaagtgt tgatggtgat accacgagct
      121 ttttcttccg gcgcgttatc gatctggtcg aatgcgcgag cagcaccgcc gtaggtttta
      181 gccagtacgg tagtgattgc agcggtcagc gttgttttac catggtcaac gtggccgata
      241 gtaccgacgt taacgtgcgg ttttgtacgt tcaaactttt ctttagacat cgattgtccc
      301 tctaagacac ggataaatcg gtgatatcac cacatcaacc aggcaacatg cccgacttgt
      361 tgaatgcaat aaacagaagg aaaaaacagg gaggagaaaa gaagtggtgc tgataggcag
      421 attcgaactg ccgacctcac ccttaccaag ggtgcgctct accaactgag ctatatcagc
      481 acatcttgga gcgggcagcg ggaatcgaac ccgcatcatc agcttggaag gctgaggtaa
      541 tagccattat acgatgcccg catcctggaa ctcggctacc tgattttcat tctgcactga
      601 atatcgagag aagctctctt tattcgagcc ggtaagcgaa cttatcgtct cgggctacgc
      661 catcgcgtgg ccgaaattgg tggtggggga aggattcgaa ccttcgaagt ctgtgacggc
      721 agatttacag tctgctccct ttggccgctc gggaacccca ccggacttga tggtgccgac
      781 taccggaatc gaactggtga cctactgatt acaagtcagt tgctctacct actgagctaa
      841 gtcggcatca agtagcgcgc attctatgga gacatgcgag ttcatgcaac taaaaaattg
      901 cataatttgt tttattggtc acattttatg cgacacgatg aagaaacagc cgaatggagg
      961 gcagattagc acactttttc aacatcattg tgctcaacaa tgcgctcctg ctaaaccata
     1021 attcttttta tcagatggaa tatctgtcac attgcttttc aacgatagct tcctgggaga
     1081 gattttttct tattattcct ccccatctgg tgttaccctc ctgcccatta acccattcaa
     1141 cagaactgtg acgcgccatg gcaaatatcg ctttgccgat agagctatga ccgccagaaa
     1201 catgcttatg agtataaaag agcaaacgtt aatgacgcct tacctacagt ttgaccgcaa
     1261 ccagtgggca gctctgcgtg attccgtacc tatgacgtta tcggaagatg agatcgcccg
     1321 tctcaaaggt attaatgaag atctctcgtt agaagaagtt gccgagatct atttacctct
     1381 gtcacgtttg ctgaacttct atataagctc gaatctgcgc cgtcaggcag ttctggaaca
     1441 gtttcttggt accaacgggc aacgcattcc ttacattatc agtattgctg gtagtgtcgc
     1501 ggtggggaaa agtacaaccg cccgtgtatt gcaggcacta ttaagccgtt ggccggaaca
     1561 tcgtcgtgtt gaactgatca ctacagatgg cttccttcac cctaatcagg ttctgaaaga
     1621 acgtggtctg atgaagaaga aaggcttccc ggaatcgtat gatatgcatc gcctggtgaa
     1681 gtttgtttcc gatctcaagt ccggcgtgcc aaacgttaca gcccctgttt actcgcatct
     1741 tatttatgat gtgatcccgg atggagataa aactgttgtt cagcctgata ttttaattct
     1801 tgaagggtta aatgtcttac agagcgggat ggattatcca cacgatccac atcatgtatt
     1861 tgtttctgat tttgtcgatt tttcgatata tgttgatgct ccggaagact tacttcagac
     1921 gtggtatatc aaccgttttc tgaaattccg cgaaggggct tttacagacc cggattccta
     1981 ttttcataac tacgcgaaat taactaaaga agaagctatt aagactgcca tgacgttgtg
     2041 gaaagagatc aactggctga acttaaagca aaatattcta cctactcgcg agcgcgccag
     2101 tttaatcctg acgaaaagtg ctaatcatgc ggtagaagag gtcagactac gcaaataatt
     2161 tgcaggggag ctaatgctcc cctttcttat ttttctgcac tacgcaggga tatttcaccg
     2221 cccatccagg gttttattat tccgtcctgc tcaagcaata aagcgccttg tttgtctatt
     2281 ccgcgtgaaa tgccaaatat ttctttatca ccaatgataa gtttcactgg gcgattaata
     2341 aaattatcca gcttttccca gcgcgaaaga taaggtgcca atccttcttg ttcgaagagt
     2401 tccagcgccg cgcgtaattc acgtattagc atggccgcca acgtattacg atcgagattg
     2461 atccccgctt cctgcagcgt gatccacccc tgattaacga cactctcttc aacacgacgc
     2521 attgccatgt tgatcccggc tccaatgact atttgcgccg catcgccggt tttgccagtc
     2581 agctccacaa gaatgcctgc cagcttgcga tcctgcagat agaggtcatt aggccatttg
     2641 acacgaactt tatctgctcc cagcttgcgt aatacttccg ccatcacgat accgataacc
     2701 agacttaaac caatcgccgc cgccgggcct tgttccagac gccagaacat cgacaaatat
     2761 aagtttgcgc caaaaggcga aaaccatttc cgaccccggc gaccacggcc agcctgctgg
     2821 tattctgcaa cgcaggcatc gcccgattta agctctccga tacgatcaag aaggtactga
     2881 ttcgtggagt caatcactgg cagcacagtt acactaccgc catccagctg acccaatatc
     2941 tgttcagcat taagtaactg gataggctca ggcaggctgt atcctttacc cggaacggta
     3001 aagacatcaa cgccccagtc acgcagtgtc tgaatgtgtt tattaatagc cgcccggctc
     3061 attcctaacg tttcacccaa ctgctcacct gagtgaaatt cgccattcgc taacagcgcg
     3121 attaatttca gtggcacagt gttatccttc atgaaattgt ctccactgcg ctcacttcac
     3181 ctgatgcacc aataaagcgg acttcaggct caagccagac attaaatttt tcacccactt
     3241 tttgtcttac atggtgtgcc agttgcacca catcttcgct ttttgcattg tcttcattaa
     3301 tgagaactaa cgcctgttga cggtgcaccg cagccccacc catttgcatc ccttttagct
     3361 ggcactgatc gataagccaa cctgctgcca gttttactga accatccgcc tgggggtaat
     3421 ttggtgctgt tggaaattgt gacagtaatg ctttagccgt ttcagcagat acaacagggt
     3481 ttttgaagaa actaccggca ttgccattca cttttggatc aggaagtttg gtggtgcgca
     3541 tatgacacac cgcatcaaat acttgctgtg gcgttactgt tgtaggatcc agacgagtta
     3601 agtcaccata cgttagtaca ggttgccact cttttggcag acgcagacct acggctacaa
     3661 tggcgaagcg gtcctggtat tcatgtttaa aaatactgtc gcgatagcca aaacggcact
     3721 ctttggcagt taagcgcact tgcttgcctg tcgccagttc aacacaatca acataagcgc
     3781 aaactcgctg taattctacg ccataagcac caatattctg gataggtgat gagccgacac
     3841 gaccaggaat taatgccaga ttttccagac caggcatacc ttcctgcaaa gtgtatttta
     3901 ccaggcgatg ccagttttct ccggctccta catgtaaata ccacgcatca ggttcatcat
     3961 gaatttcgat acctttgatc cggttgatga tcaccgtgcc gcgatagtct tccagaaaaa
     4021 gtacattact tccttcaccc agaataagaa cgggttgccc ttctgcggtt gcatgctgcc
     4081 aggcattgag tagttgttgt tcgtcttcgg cacatacaat gtgctgagca ttatgatcaa
     4141 tgccaaatgt gttccagggt tttaaggagt ggttcatagc tgctttcctg atgcaaaaac
     4201 gaggctagtt taccgtatct gtggggggat ggcttgtagt tatgacgaca gggaaagttt
     4261 gtagaaacgc aaaaaggccc agtcttccga ccgggccttc tgctt
//
LOCUS       DAZFFG010000227         4261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFFG010000227 DAZFFG010000000
VERSION     DAZFFG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.228"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..978
                     /locus_tag="ACHJ7J_004889"
     CDS             <1..978
                     /locus_tag="ACHJ7J_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5002983.1"
                     /translation="KGTTLGNQLEVIPADRTWRPRLQNKPKVDGPQSAIVTGPAGEEI
                     FCDEHGRVRVKFHWDRYNPATEASSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNG
                     DPDQPIIMGRTYHEDNRSPGSLPGTKTQMTIRSKTYKGSGFNELRFEDATGGEQVYIH
                     AQKNMDTEVLNNRTTDVKVDHTETIGNNQSITVGLGQTVTVGKENAGGHDQTVTVTHD
                     QNVSVGNDQSLTVTNNRSKSVGNDQDSKVTGNDTEAVDKSQSVTVGETLTVTVGDSIE
                     FVCGKSTLRMDKDGYITINGHELSLGTTGDQFYKADGDMNMKGKKILEN"
     gene            978..1520
                     /locus_tag="ACHJ7J_004890"
     CDS             978..1520
                     /locus_tag="ACHJ7J_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HFO5002984.1"
                     /translation="MIKELHDIYVPQPEEELTSTTHLLENILSHQASKSQAHVSDIPV
                     LVVGRIVSVKDSGEILIQCPHEDKPVAALGLGTVTTEYNGRLCTVQFLSGNYSHPVVT
                     GILPENLTERYHSETKKCTEDELNTERVVLTAKQELVLQCGESKIVLSADGLLQIRAL
                     YIDSQAQATHRLKGGSVQVN"
     gene            1533..2675
                     /locus_tag="ACHJ7J_004891"
     CDS             1533..2675
                     /locus_tag="ACHJ7J_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HFO5002985.1"
                     /translation="MEIFNAAKHTIADVFTATDKNGKDYLVVVIKATYQIPVNNRRPR
                     PLLPPQPLVESDIYVGEPGFSAVLYEADYVRYKEKCDVLFNATTYFVSEGNNYQTESR
                     AIVGQVDKSLKVFGPRYWQKKTSDWIISDTEFIQSVSLHYGNAFGGEYVWKDVQGNEN
                     REIFSSNIVGIGYGNHRDETVFINSRLPLVELPQDSVTRPESHPEPGAFSARPRNHPL
                     RLQYAGTYDERWRAEVSPFLPEDFDERYFQSAPPDQQTAYPQGGEKVMLINMHKERSV
                     IEFQLPHINQMPVRILTMDYEAHELMPVVDTLYFEPNEERFSVVWRTSIPLKRRIQEI
                     HTVAIGPVGKTWWQQRAAGGGETCGGCGEKNNNPELTNKPVTETGA"
     gene            2681..3694
                     /locus_tag="ACHJ7J_004892"
     CDS             2681..3694
                     /locus_tag="ACHJ7J_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002986.1"
                     /translation="MASPLFIAAAGMCCSIGYSVPSASAAIRSGLDHFAETTFYDLNG
                     KPLIGAQLYKNDTWGDERLLWMFENVVAECILKTPGMTKKNTCLIFLLSECAEQCYGS
                     SGNEFLFSRVKTTWHESSICIQGGKTGIGSAITEAQRILYSGLASYALVVGVDSYFSP
                     ARINYLLTHERVLCTSNRDGFIPGEGAGAIVFCKPDSCIQGICITGVGVSNEDAHILQ
                     NDKANMAKGLSVAIRKAIQDAGVLLAETDFHISGVTGESWYFRETALAITRCLEKKKK
                     QYPHYQLASNLGETGAASGPLTLAYLNEMMCRFPDTGERALVHFSGDSGLRVALVAEY
                     YRG"
     gene            3699..>4261
                     /locus_tag="ACHJ7J_004893"
     CDS             3699..>4261
                     /locus_tag="ACHJ7J_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HFO5002987.1"
                     /translation="MDTRVYINNREACSKASDGVSKAAFPDPCWTPPSPPAGPIVVPY
                     PNTAYASTLKDGTTTVFVCNSMVAKEDVSCFSTSTGDEPATQSQPKGVITGVIKGKAY
                     FRTWSPNVKFEGKCVARHEDLMSHNHGSQPGNTDLFYYLSEPISVGNRIWDNVLKMLC
                     WQDNYEKEVSVNITKRYADAFDSNGLS"
BASE COUNT         1264 a          813 c         1093 g         1091 t
ORIGIN      
        1 aagggaacca cgctgggaaa tcagttagag gtgataccgg cggaccggac gtggcgtcca
       61 cggctgcaaa acaaaccgaa ggtggacggg ccacagagcg ccattgtcac cgggcctgcg
      121 ggagaagaga ttttctgcga cgagcatggc cgggtacggg tgaagttcca ctgggacagg
      181 tataacccgg caacggaggc gagttcatgc tgggtaaggg tgtcgcaggc gtgggcgggg
      241 ccggggtttg gtaacctggc gataccgcgg gtgggtcagg aggtgattgt tgacttcctg
      301 aacggagacc cggaccagcc gataatcatg ggccggacat accatgagga caaccgttcg
      361 ccggggagtc tgccggggac gaagacgcag atgacgatac ggtcgaagac ctataaagga
      421 agcggattta acgagctgcg gtttgaagac gccacgggcg gtgaacaggt ctatatccat
      481 gcacagaaga acatggatac ggaagtgctg aacaaccgca ctactgatgt gaaagtggac
      541 cataccgaaa ccatcggtaa caaccagagc attaccgttg ggctgggcca gacggtgacg
      601 gtgggtaagg agaatgccgg gggacatgat cagaccgtca ctgtaactca cgatcaaaat
      661 gtaagcgtag gaaatgatca atctctgact gtcacgaaca atcgtagcaa gagcgtaggt
      721 aatgatcagg atagtaaggt aacagggaat gataccgagg ctgtggataa atctcagtct
      781 gttactgtag gggaaactct tacagtgaca gtaggcgatt ccatcgagtt tgtatgtggt
      841 aagagtactt tgcgcatgga taaagatggc tacatcacta taaatggaca tgaattgtcg
      901 ttgggaacaa caggtgatca gttttataaa gctgatggtg atatgaatat gaaagggaag
      961 aaaattctgg agaactaatg ataaaggaac tgcatgatat atacgtccca cagccagaag
     1021 aggaactgac cagtacaaca catttgctgg aaaatatttt atctcatcag gctagtaaat
     1081 cccaggcgca tgtcagtgat atccctgtgt tggttgtcgg cagaattgtc agtgtcaaag
     1141 actctggaga aatattgatt cagtgtcctc atgaagataa acctgttgca gctttgggtc
     1201 tgggaactgt aactacggag tataacggac gattatgcac tgtgcagttt ctctctggca
     1261 attattctca tccggtagtg accggaatac ttccggagaa tttaacagaa agatatcatt
     1321 cagagacgaa aaaatgtact gaagacgaac taaatacaga gcgagttgtt ttgacagcga
     1381 aacaagaatt agtgctccag tgcggcgagt caaaaattgt tttatcggcg gatggactgc
     1441 ttcagatacg cgcgctttat attgacagtc aggcgcaggc aacacacagg ttaaaaggtg
     1501 ggtctgtgca ggtaaactga ggttatatta aaatggaaat ttttaatgct gcaaaacaca
     1561 cgatagctga tgttttcact gcaacagata aaaatgggaa agattatctt gtagttgtaa
     1621 ttaaagcaac ttatcagatc cccgtaaaca atcgacgccc tcgcccacta ttaccacctc
     1681 agccattggt agaaagtgac atatatgtgg gtgaacctgg attctctgct gtattatatg
     1741 aggccgatta tgtcaggtat aaagagaaat gtgatgtttt atttaacgca acaacttatt
     1801 ttgtaagcga ggggaataat tatcagacag aatcaagagc aattgtaggt caagtcgata
     1861 agagtctaaa ggtatttggt ccgcgctact ggcagaaaaa gacgtcagac tggattatat
     1921 ctgatacaga gttcatacag tcagtctcat tacattatgg taatgccttt ggtggagagt
     1981 atgtctggaa agatgtacag ggaaatgaga atcgggaaat attttccagc aatattgttg
     2041 gaatcggtta tggcaatcat cgggatgaaa ccgtattcat taactcacgt ctgccattgg
     2101 ttgaactacc acaagatagc gtgacacgtc cagaatcaca tccggagccg ggagcatttt
     2161 ccgcccggcc aagaaaccat ccgttacgtt tacagtatgc cggaacatat gacgaacgat
     2221 ggcgtgcaga agtctccccc tttcttccgg aggattttga tgagcgttat tttcagagcg
     2281 caccacctga tcaacagacc gcatatccgc agggtggtga aaaagttatg ttgataaata
     2341 tgcataaaga acgctcagtc attgaatttc agctacctca tataaaccaa atgcccgtta
     2401 gaattttaac gatggattat gaagctcatg aattaatgcc ggttgttgat acgctttatt
     2461 ttgaacccaa tgaggagcgt tttagtgtag tctggcgtac aagtatacct cttaagcgac
     2521 ggattcagga aattcacact gttgctatag ggcctgtggg taaaacctgg tggcagcaac
     2581 gagctgcagg gggaggggaa acctgcggtg gatgcggaga aaaaaacaat aatccagaat
     2641 taacgaataa acctgtaaca gaaacaggag catagttatt atggcatcac cattatttat
     2701 tgcggcagcc ggaatgtgtt gcagtatagg atattcagtt ccttctgcat cagcggccat
     2761 tcgctcgggt ctggatcatt ttgctgaaac aactttttat gacttaaatg gaaaaccact
     2821 tattggtgct caattatata agaatgatac ctggggtgat gagcggctac tctggatgtt
     2881 tgaaaatgta gtggctgagt gtattttaaa aacacctgga atgacaaaaa aaaatacatg
     2941 tctgattttt cttttatcag aatgtgcaga gcaatgttat ggctcatcag gaaatgagtt
     3001 tctttttagt agagtaaaaa caacctggca tgaatcttct atatgtatac agggagggaa
     3061 gacagggatt ggttctgcga ttacagaagc tcagcgtatt ctatactccg gacttgcttc
     3121 atatgctctt gtggtcggtg tggatagtta tttttcacca gcaagaataa attatcttct
     3181 tactcatgaa cgcgtattat gcaccagcaa cagggatggt tttattccgg gggaaggtgc
     3241 cggggctatt gtgttttgta aaccagatag ttgtattcag ggcatttgta ttaccggtgt
     3301 cggtgtcagc aacgaagatg ctcatatcct acaaaatgat aaagctaata tggctaaagg
     3361 attatctgtt gcaatccgga aagcaattca ggatgcaggg gttttattag cagaaacaga
     3421 ctttcatatt agtggtgtca ctggtgagtc atggtatttc cgtgaaactg ctctggcaat
     3481 cacccggtgt ctggaaaaga aaaaaaaaca ataccctcat taccagcttg ccagcaatct
     3541 gggagaaacc ggggcggcat ccgggccact gacactggcc tatctgaatg aaatgatgtg
     3601 tcgattccct gatacgggag aaagagcact ggttcatttc agtggtgaca gtggacttcg
     3661 ggttgcgctg gttgcagagt attacagagg ataaaaatat ggatacgcgt gtttatatca
     3721 acaaccggga ggcttgttca aaggcatctg atggtgtatc taaagctgca tttcctgatc
     3781 cttgctggac gccaccatct cctcctgcgg gtcccatcgt ggttccttat cctaacacag
     3841 cctatgcctc aacgttgaaa gacggtacaa cgaccgtatt tgtttgtaac tcgatggtgg
     3901 caaaggaaga tgtttcctgt ttttcaacca gtacaggaga tgagccagcc acgcagtcac
     3961 agccaaaagg cgtaataacc ggggtaatta aaggaaaagc ttacttcagg acatggtcgc
     4021 caaatgtaaa gtttgaaggg aaatgcgttg cacgtcatga agatttaatg tcacacaacc
     4081 acggttctca gccaggaaat acagatttat tctattattt atcagagcct atatctgttg
     4141 ggaatagaat atgggataac gtcttgaaaa tgctatgctg gcaagataat tacgaaaaag
     4201 aagttagcgt taacattaca aagcgttatg cggatgcctt tgattctaat gggctttctc
     4261 a
//
LOCUS       DAZFFG010000228         4127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFFG010000228 DAZFFG010000000
VERSION     DAZFFG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.229"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            330..593
                     /locus_tag="ACHJ7J_004894"
     CDS             330..593
                     /locus_tag="ACHJ7J_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO5002988.1"
                     /translation="MLQIIRKGDKTTHGGSVMTCSDTMIFGGIGVARKGDMVSCPIPG
                     HGPTTIIEGNPDYLDNGIPVAFHGHKCGCGCTLISSFAPAKVA"
     gene            596..1804
                     /locus_tag="ACHJ7J_004895"
     CDS             596..1804
                     /locus_tag="ACHJ7J_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002989.1"
                     /translation="MPVLLDTIPEKAAKVPRPDTRRWLLFLAFVMSGGFALTLWNWTT
                     ERTGFIFWFTALGLPFCLWGLLFSLRRFAYKAEQVGAESRNVERETLIERETCRGQRC
                     GWILDTYVQHFSGNSPGALVNAVNHTVPASETSTPRGSKSAVRYAALPGFQTALDTEL
                     ISVTSTLVTHVQKITDTLSKDISCCLMLDCDDDIRPGLEEHFRNELTTRTGRVFRLLA
                     GKGLAGFDAWLDQRWDTPGILAGVTFSLPAQPDEGDADAITLVVLSNRRVAGYPDAVC
                     LHRPEKGKEARLVKTLNRALLWAATAPEALKTAWYTGPVLSGGSGWNMACEDNGVTFS
                     LSKDNHGIDFALGYARRAAPWLAVILASVACRDNGPQVISAQSTTDKDDVWVAVITKE
                     EVRKESPGNG"
     gene            1797..>4127
                     /locus_tag="ACHJ7J_004896"
     CDS             1797..>4127
                     /locus_tag="ACHJ7J_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO5002990.1"
                     /translation="MAKVKVKTLIGSVVTVAFFLTVIVAFLFYAFPENVALATGFGPY
                     DGQRILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIPDEE
                     DDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLCHDLWQEGDGY
                     VLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALPTDGERDALLRYRQKAD
                     HQLGWQAPVWLWLTDNATGARQDAKMAATGVIFGPEGTVKGADEAFSTLALHLQKAGM
                     AQILNDPADFGFLQLSLRLQQELKTSLAVFLSGLMQGSAAWRLRGVMFSPELTVAGAV
                     PNTCPDTPAWKAIIDDCDAVSGRKPGFNWLKMLRLILLSLIVLWGAGTLLSLLVNRTQ
                     IYQAQETARQAADTTKPLAERLRSQLVLQQAIARLQHREATGAPWYTRFGLNQDSDTL
                     AALWPLYAKNNAQLMRDATADSLRQQLNAFVQLPPASDARTQGTQHTYDILKSYLMLA
                     RPDKADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFYAQNLPAHPEWKIKPDDEL
                     ISTVRQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLADMTGDTDASTVFSTDEVVP
                     GMFTRQAWEEQVQDAVNEVVRTRRDEIDWVLTDKTHQTGSDISPEALKTRLTERYFTD
                     FGNAWLNMLNSIQWHEPTSLSEAIAQLSLIGDVRQSPLVALMNTLNYQGKTGQKGEAL
                     ADSIVDSAKKLLDKNKNRRQFIEQAQGSEGPLDGVFG"
BASE COUNT         1003 a         1013 c         1127 g          984 t
ORIGIN      
        1 agttgttctt ggattcagat agggatccca atttatcagg ttttcaaaca aaacataata
       61 aaaaggaatc taactataaa ttagatagtg cgagtaaaat aagggagtat ttacgcacta
      121 aatatggaga atgatttcag tatgctaagc atcgacttga gttgggtgtt aatgattgac
      181 tagcaagacg aatttccatg agaaataagt gtattttatt gattctcgct gcgattatat
      241 tctctttgcc agacagcaat aataagacca gtgtttcaat aaatattaat ttattctgtc
      301 ctgtaaacaa taaattaacg gagatacaaa tgttgcaaat cattagaaaa ggcgacaaaa
      361 ccactcacgg cggctctgtt atgacctgtt ctgacaccat gatatttggt gggattggcg
      421 tggcacgtaa aggtgatatg gtttcctgcc cgatacctgg acatggcccg acaaccatca
      481 tcgaaggcaa tcctgattat cttgataacg ggatcccggt tgcctttcat ggtcataaat
      541 gtggttgtgg ctgtacattg atcagttctt ttgcacctgc aaaggttgcc tgagtatgcc
      601 agtcctgctg gataccatcc ctgaaaaggc ggcgaaggtt ccacgacctg atacccgtcg
      661 gtggctgctg tttctggcgt ttgtcatgtc aggaggattt gcgttaacgc tctggaactg
      721 gacgacagaa cgcacaggct ttattttctg gtttactgca ctgggattac ctttctgtct
      781 ctggggactg cttttctccc tgcgccggtt tgcatacaaa gcagagcagg tgggggctga
      841 gtcccggaat gtggagcgcg aaacgcttat tgagcgcgaa acctgtcggg ggcagcgctg
      901 tggctggata ctcgatacat acgttcaaca cttttcagga aatagtcccg gtgcactggt
      961 taacgcagtt aatcatactg ttccagcttc agagacctca acgccacgtg gttccaaatc
     1021 tgctgtcagg tatgcagctt tacccggttt tcagactgct cttgatacag aactcatctc
     1081 tgtcacgtca acactcgtca cccatgtaca aaaaattact gacaccctgt cgaaagatat
     1141 ttcttgctgt ctgatgctgg actgtgacga cgatattcgt cccggactgg aggaacactt
     1201 cagaaatgaa ctcacgacca gaaccggcag agtcttcaga ctccttgcgg gcaaaggtct
     1261 ggcggggttt gatgcctggc tggaccagcg ctgggatacg ccgggcattc ttgcaggcgt
     1321 gacgttttca ttgcctgcgc agccagatga aggtgacgcc gatgccatta ctctcgtggt
     1381 gctcagcaac cgtagggttg ctggctatcc ggatgcagta tgccttcatc gccctgaaaa
     1441 gggcaaggaa gcccggcttg tgaaaacgct gaacagggca ctgttatggg ccgcaacagc
     1501 ccctgaagca ctaaaaacgg cttggtatac aggtccggtt ctttcaggtg gaagtggatg
     1561 gaatatggcc tgtgaagata acggggtgac attcagtctg tcaaaagaca atcatggcat
     1621 tgattttgct ctgggatatg ccagacgtgc cgccccctgg ctcgctgtaa ttcttgcctc
     1681 agtggcatgc agagataatg gcccgcaggt tatctccgcc cagtccacca ctgacaaaga
     1741 cgacgtctgg gtcgcagtca tcaccaaaga agaagttcgc aaggaatcac ccgggaatgg
     1801 ctaaggtaaa agtaaaaaca ctcattggct ctgttgtaac tgtcgccttt tttctgacgg
     1861 tcatcgtggc atttcttttt tatgcctttc cggaaaacgt cgctttggcc actggcttcg
     1921 gtccgtatga cgggcagcgc atactcacat tctgcgccat actggttgcc acgttgtttt
     1981 tactcggctg gctgacggag aaggctttcg acttcgctgg taaatccggg ctgtatttcc
     2041 agtgggggca gaaaaaggct gagagtatca ctccggtgac gaataccatt cccgatgaag
     2101 aagacgacga tgagccggtt ttcagcgaag atgctgtttc agaacatttg cgctttcgct
     2161 atgggcgccg ctggcagcgt aaggtgcgac tcctgctggt gatgggggat ccggatgtgg
     2221 tccggaaagc ggccccgggc ctttgccatg acctctggca ggaaggtgac ggctacgtgc
     2281 tgatttatgg cggagacgcg cagtcactcc cggacgagat cttcctttca aaactgaagc
     2341 gtctgcgatc cgggcaaccg gttgatggca tcattcaggt gatgagtatt tcagccctcc
     2401 cgacggatgg cgaacgggat gccctcctgc gttatcgtca gaaagccgac catcagcttg
     2461 gctggcaggc gccggtctgg ctgtggctga ctgacaacgc taccggtgcc cggcaggatg
     2521 cgaaaatggc cgcaacgggg gttatcttcg gaccggaagg aactgtgaaa ggtgcggacg
     2581 aagcattttc cacactggcg ttacacctgc aaaaagcagg aatggcacag atactgaatg
     2641 atcctgctga tttcggtttc cttcagttgt cactcagact gcagcaggag ctgaaaacca
     2701 gtctggctgt tttcctcagt ggactgatgc agggatctgc ggcctggcgt ctacgcggtg
     2761 tcatgttcag ccctgaactg accgttgccg gagcggtacc aaacacatgc cccgacacac
     2821 ctgcctggaa agctattatt gatgactgtg atgccgtcag cggcaggaag ccgggcttta
     2881 actggctgaa gatgctgcga ttgattctgc tttccctgat tgtcctgtgg ggggccggta
     2941 cactgctgtc tctgctcgtt aaccgtactc agatttatca ggcgcaggaa acggcgagac
     3001 aggcagcgga tacaacaaaa ccactggctg aacgcctgcg cagccagctt gtactccagc
     3061 aggccattgc ccgtctgcaa catcgtgagg caaccggtgc gccatggtac acccgctttg
     3121 gtcttaatca ggacagcgac acgctggccg cgctctggcc gctgtatgcg aaaaataacg
     3181 cacagctgat gagggatgcc acagcagaca gcctcaggca gcagctgaat gcctttgtgc
     3241 aactccctcc tgccagtgat gcccgtacgc agggcacaca gcatacttac gacatactca
     3301 aaagttatct gatgctggcg cgtccggaca aagcggacgc cggctggctg gcaaaaaatg
     3361 cgctgaaagc cttaccgaaa cgccggaatg tgccggatgg catatggcag aacgtcgcac
     3421 cgaaactgct ggggttttat gcgcaaaacc ttccggcgca tccggaatgg aaaattaagc
     3481 ctgacgatga attaatcagt acggtccgtc agattctgct taagcaaatt ggccagcgga
     3541 atgctgagtc cgggttatat caggagatgc ttaagcgcgt ggccagcaac tggcccgatt
     3601 taacacttgc ggatatgacc ggtgacaccg atgcctcaac ggtattcagc actgacgaag
     3661 tcgttccggg aatgtttacg cgccaggcct gggaagagca ggtacaggat gcggttaacg
     3721 aagtcgtcag aacacgccgc gatgaaattg actgggtact gacggacaag acgcatcaga
     3781 cagggagcga tatctctccg gaggcgctga agacccgcct gactgaacgc tattttaccg
     3841 attttggtaa tgcctggctg aacatgctca acagcataca gtggcatgaa cccacgtcac
     3901 tttccgaagc cattgcccag ttgagtctga tcggtgatgt ccgtcagtct ccgttagttg
     3961 cactgatgaa cacgctgaac taccagggga aaacggggca gaaaggcgag gcactggctg
     4021 attcaatcgt ggattcagcg aaaaagctgc tcgataagaa taaaaatcgc agacagttta
     4081 ttgaacaggc acagggctct gaaggcccgc tcgatggggt atttgga
//
LOCUS       DAZFFG010000229         4108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFFG010000229 DAZFFG010000000
VERSION     DAZFFG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.230"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            72..1037
                     /locus_tag="ACHJ7J_004897"
     CDS             72..1037
                     /locus_tag="ACHJ7J_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HFO5002991.1"
                     /translation="MPLDLSNTLVIGISATALFDMSESDRMFRETYATDPDSAIEKYR
                     AYMLEHENQPLQPGTGYPLVKALLGLNQYRQPDDQSPLVEVVVMSRNSPDTGIRVLNT
                     IRHDKLDITRSAFTAGETVADYLDAFDVDLFLTTNVEDAQKVIDSRFCAAAILKDPPS
                     DASDIPEGQVRIAFDGDAVLFSDQSELVYKTQGMAAFHAQEDAQQDIPMEEGPYATLL
                     KKIAKLQERLPTRVEYSPVRIALVTARNSPSEMRVIKTLRSWGVYVDEAFFLGGVEKT
                     KVLKAFRPHIFFDDQDVHLIAAAKVVPSGKVPYASNSELAEREAS"
     gene            complement(1115..1990)
                     /locus_tag="ACHJ7J_004898"
                     /pseudo
     CDS             complement(1115..1990)
                     /locus_tag="ACHJ7J_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465694.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
     gene            complement(2196..2376)
                     /locus_tag="ACHJ7J_004899"
                     /pseudo
     CDS             complement(2196..2376)
                     /locus_tag="ACHJ7J_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            2659..3027
                     /locus_tag="ACHJ7J_004900"
     CDS             2659..3027
                     /locus_tag="ACHJ7J_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002992.1"
                     /translation="MSAQEKTVQIDSFDAKFLDINTLSKLIYERKSFVIENVSDVSET
                     VKRVEHEIEKRKLSCRVYTEYRSTALAGSLWSPTVILGVASAVAIGVHNLSTWNPDYE
                     IGKNYIKRRLSVKYKKAEVL"
     gene            3244..3990
                     /locus_tag="ACHJ7J_004901"
     CDS             3244..3990
                     /locus_tag="ACHJ7J_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO5002993.1"
                     /translation="MKKVFAVSVVGAACVFAANAGAEEGKSGFYLTGKAGASVVSLSD
                     QRFLSGDEEETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NTDKGKGIWSDGYWRDDLKNEVSLNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKT
                     TGISTWDYGYGYSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSS
                     VSYKDEWGDKYKSEVDVKSHDILLGVTYNF"
BASE COUNT         1114 a          904 c          945 g         1145 t
ORIGIN      
        1 attatttatg aagtaagttc ctttaatagg attccataac ttcattgaag aactatccaa
       61 ggaacctggt tatgccttta gatttaagta acactttagt tattggcatt agcgcaactg
      121 cgctttttga catgagtgaa tcagatcgca tgtttcggga aacctatgcg accgatcctg
      181 atagtgccat tgagaagtat cgagcctaca tgctggagca cgaaaatcag cctttacaac
      241 caggaacagg ttatccctta gtcaaagctc ttctgggtct gaatcagtat cgtcaaccgg
      301 atgatcaatc ccccttggtt gaagtcgttg tgatgtcgcg taatagtcct gataccggta
      361 tccgcgtttt gaataccatt cgccatgaca aactcgatat tacacgttca gcttttaccg
      421 caggtgaaac cgttgccgat taccttgatg cctttgatgt ggatctgttt ttaacaacca
      481 acgttgaaga tgcgcaaaag gtgattgatt cccgattctg cgccgctgca atcctgaaag
      541 atccgccttc tgacgcatct gatatcccgg aagggcaagt gcgcattgct tttgacggcg
      601 acgcggtact tttttccgat caaagcgaac tggtttataa gacccaagga atggctgcct
      661 ttcatgctca ggaagacgcg cagcaggata tccctatgga agaggggcct tatgccactt
      721 tgctaaaaaa aatagccaag cttcaggaac gattgccgac ccgcgttgag tattctcctg
      781 ttcgtattgc gctggtgaca gcccgaaata gcccttcgga aatgcgcgtt attaaaacgc
      841 taagaagctg gggggtatat gtcgatgaag ctttttttct tggcggtgta gagaaaacaa
      901 aagttctgaa ggcgtttagg ccacacattt tctttgacga tcaggatgtg catttgatcg
      961 cggctgcaaa ggtagtacct tctggtaaag taccttatgc cagtaactcc gaactggcag
     1021 aacgtgaagc atcgtaatta tcgctaacaa accatttagt cattaaggct ggatggtttg
     1081 tataccacac gcaaaataac tgccctaccc cagatcaatg ctctcctcta aacacctctt
     1141 tccagtgcca ttccacaaac tgttcgcccg gataattttc tttaaccggt ggcaacgcaa
     1201 tcgcttttcc cgcaaaatcc cagaacagtc actgtaccac tccaccacca tttaccgcac
     1261 cggaaaccac cacacgcata ttttcatcca ggtcaatcga accgcgatca aacgctttat
     1321 gatggattgc gcacaatgcc aggccattag gtacttcaca agggccatgg tgctgtttcc
     1381 atctgatatg agcagcttcc agatcaatcg gcgcattgtc gtggcgcatg ttaaagccgc
     1441 agatagcaca ctgataattg taagcatgta gcaccatctg gcgaaacttc ggatcgcgtt
     1501 gacgaagaga ggtgcgaata tcaaaactcc tctcgtcagc tatctcttcc tgaatactgg
     1561 ccgggaaatg cgcctctagg agctgctgcg ccagcgtatc aattaacctg taattttttt
     1621 ccaccaacgc aaaattatcg gcatcaaatc caccagcgac attatattca atgagttcac
     1681 gtcggggcgg ctggcggcta ccactggttg agcacagttc cgcgttatgt agttcccaga
     1741 agccatcgcc cttcagacgc cagaacagca tgtccgggcg ctggtcacgg cgctgcggtc
     1801 cataacgttc taggagatcc aacaggggct cgtagatttc agaagcatag ttaaacaggc
     1861 gaccatgacc ctgccggtag tgcgagagaa cgcacagcag caataatgga ttatgtggcg
     1921 aacgctgttc gtctttgcgc cagatggtga tatttgatat cgcttgcttt agggttttgt
     1981 ctgaggtcat tgcgttggag aatccatgag cacatgtttg cgatgatgct cgcaactata
     2041 tgtacattca aggcattatc gcagacatcc tttttgcctg acggctctgt acctggcaga
     2101 tggaattctg aaaaaaatac agcgaccaac cagtcctaag cggtctggtt gatcttttaa
     2161 atttaaacat cagccaagga gtaacggcat tcaactcact cgccattacc ccgattgatc
     2221 ttcgcatagt caaacggcgt aatcgctctt tcccgattgg catccagaaa atcgacccac
     2281 cactgcaaca tcagcttacg ctcatccaga tattccgctt tatggattat acgcggcctg
     2341 cactgaattt cgctccatgt ggctcatctg acgttcagcc gctttatctc gtgctgtttt
     2401 ggtctcaata cttccatgcc gttgcaatta cctcttaaac cattccttac cattttcatg
     2461 tcttcgataa gaaatcttca tcatgcatga tcgattttgt cgatcgattc gatcgatttt
     2521 tctcttttta ccctgccttt tgcatggttt taaaataggc aaaattactt gtcattcgtg
     2581 gaaaatgatg gcggagataa ctgagcttaa aaagtttctc ttgtctccgt cacttgccat
     2641 accagccagg aaaagaagat gtcagcacaa gaaaaaacag tacagattga ttcttttgat
     2701 gcgaaatttt tagatataaa tacattaagc aagcttatct atgaacgtaa gagttttgtg
     2761 atagaaaatg tttccgatgt tagtgaaacg gtaaaaagag tggagcatga aatagaaaaa
     2821 aggaaattaa gttgtcgtgt atatactgag tacaggagca cagctttagc tggttcttta
     2881 tggtcaccaa ccgtaattct gggagtggcg agtgcagtag caataggtgt tcataattta
     2941 tctacatgga accccgatta tgaaatcggt aagaactata ttaaacggag attatcggta
     3001 aaatataaaa aggcagaagt attgtgatcg gttactccag tgcccctgcc tgttcgtatt
     3061 ctggtgaggt tgtatacaca taatttcaga ctggcgttca cgtcctcact gaggaaatat
     3121 atttattttg tgtagagtaa aagtctggct tccgtagtgt ggagggggga ggctgcctta
     3181 gtccgttttt taaatattaa aaatatatat taaagtgatg atacaataac ggagataatt
     3241 gaaatgaaaa aggtttttgc tgtttcagtg gtgggcgcag cctgtgtatt tgcagcaaat
     3301 gcaggtgctg aggaaggtaa aagcggtttt tatctgaccg gtaaagccgg tgcctctgtg
     3361 gtgtcacttt cagaccagcg tttcctgtca ggagatgaag aagaaacgtc aaagtataaa
     3421 ggtggggatg accatgacac ggtattcagt ggcggtatcg cagccggtta tgatttttat
     3481 ccgcagttca gtattccggt tcgtacagaa ctggagtttt acgcacgtgg aaaagctgat
     3541 tcgaagtata acacagataa aggtaaaggc atctggtcag atggttactg gcgtgatgac
     3601 cttaagaatg aggtatcact caacactctg atgctgaatg catactatga cttccggaat
     3661 gacagtgcat tcacaccatg ggtatccgca gggattggtt acgccaggat tcaccagaaa
     3721 acaaccggta tcagtacctg ggattacggg tatggataca gtggtcgtga atcgttgtca
     3781 cgttcaggct ctgctgacaa cttcgcatgg agccttggcg cgggtgtccg ctatgacgtc
     3841 accccggata tcgctctgga cctcagctat cgctatcttg atgcgggtga cagcagtgtg
     3901 agttacaagg acgagtgggg cgataaatat aaatcagagg ttgatgttaa aagtcatgac
     3961 atcctgcttg gtgtgactta taacttctga cgacactgct tctgaacgat aattgcgcat
     4021 atttctgcaa ttaagataat tacgtatttc tgtaattaaa aaggaacacc ctgcagtaat
     4081 attatactgc agggttgtct gttatctg
//
LOCUS       DAZFFG010000230         4103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFFG010000230 DAZFFG010000000
VERSION     DAZFFG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.231"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            836..1075
                     /locus_tag="ACHJ7J_004902"
     CDS             836..1075
                     /locus_tag="ACHJ7J_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFO5002994.1"
                     /translation="MNEDEIYRHIRQALSSAPRNQYTVELHLQMIKYADELEHITAKA
                     FCEGTGLSQSLGTEFSKMRNLTRRLKAAGLNTDLL"
     gene            1115..3553
                     /locus_tag="ACHJ7J_004903"
     CDS             1115..3553
                     /locus_tag="ACHJ7J_004903"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO5002995.1"
                     /translation="MAIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDK
                     YKGDPYGMIVIPKGASFDDMVALKNDKEIGDKINKIIHKLAEENGLKGVIDEADFNDE
                     DKLGKGKEMIDRLSKLVGIFEGLDLSSNRAEGDDLLGDAYEYLMRHFATESGKSKGQF
                     YTPAEVSRILAKVIGITPDTPRDATVYDPTCGSGSLLLKVNDEARRGLSIFGQEMDNA
                     TSALARMNMILHNNATAKIWQGNTLSDPQWKEANGKLKAFDFAVANPPFSNKNWTNGL
                     TPKKDPFERFGWGIPPEKNGDYAFLLHIIKSLKSTGKGAVILPHGVLFRGNAEANIRE
                     NLIKQGYIKGVIGLPANLFYGTSIPACIIVIDKEHAHSRKGIFMIDASRGFIKDGNKN
                     RLRSQDIHRIVDVFNHQRTVPGYSRMVPSSEIAGNDYNLNIPRYIESGDPEDLHDLTA
                     HLQGGIPARDVDALKDYWRIFPALRNVLFADDRPGYCRAQVDAQQVKPTILAHQEFKD
                     FATRSLLPFKSWVKEAGLEEIRKGDKPKALIHDISEMLLAQYANSELLNKYSVYQILM
                     DYWANVMQDDVYVIMQDGWQAAAQIRELQPVKGKDGKNIWKETHDFEFTKRRYKSDVL
                     PRSLVEIRCFPELLDALNRAQERSDEASRQLAEFIEEQAGEEDLLAEAKNDKDKVTQK
                     LVNARLAQLKKTTAGPDELAVLNRCLALIKAEASAKKALKAAQEALDRAVFKHYPTLD
                     EAAIKTLVVQDKWLATLQAGIKAEIERITQQLASRVKELEERYAEPLLALEASVEALS
                     EKVAGHLRAMGLEW"
     gene            3553..>4103
                     /locus_tag="ACHJ7J_004904"
     CDS             3553..>4103
                     /locus_tag="ACHJ7J_004904"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5002996.1"
                     /translation="MVDLMVNEQRSGTAGKDVPAGYKLTEVGVIPEDWDCVPFGNLFK
                     TNTKKKKVSDYELVSFIGMQDVSEDAQLKNNTQLPFKEVKSGFTYFEKGDVLLAKITP
                     CFENGKGCHTADLPTNVGFGSTEFHVLRENEDSDSRFIYFWTTDKKFRASLESEMVGS
                     AGHRRVPLVAIEKYLIPCPPNLQ"
BASE COUNT         1173 a          912 c         1105 g          913 t
ORIGIN      
        1 catcggaggt actaacttcc gctcctcgct caaagcagac cattcgctca gttaacgcat
       61 cagtgtcgta ctagaagtag aagtggttaa caataatgtg agttaatcaa cggagagcag
      121 gtcaactcta acctgtatca aaaaaacaaa tgtatcataa agatgccgca tgccttaatt
      181 tgagtataat tgagacggaa gtaaacggtt tatgactaga tattgttgat atatagctat
      241 ttgaagagtt gtagcgcaag cgcatagccc aaactaataa cgctacgccc cttatcaaag
      301 agcggcaaca atgcacagct cgcacgagac aatattcgca caagaaatca ggttcggaaa
      361 taacctgctc tggcatggcg gggagagtat caatggtcag gctttcggta agatgcggcg
      421 ggatgaggtc ggcgtgggtt acagtagtac aaggtcgccc accgagggta agccacagcg
      481 tggaatcctc ggtttctatc agagattcgg cgaagcaggc gcagttgtcg accaagacga
      541 tgaaatccat tgtggcatta taagggaacg aacaatagat cgagtaaatc gcagtcaggg
      601 acaaggattg tagtcataga gaagtctcct gtaacggttt tcagaaggct gatcccgacg
      661 cctgatttcg caggcgcgga aagaaagaga ggatactgcg accattaatc cgccacaagg
      721 catttacgta cataaatcgc gcagtcagat ccgactccca ctgtagttct tgaacctgat
      781 accacattta gctaatttag ctaacttatc cagtatacca gtggttgcca tgaatatgaa
      841 tgaagacgag atttatcgcc atatccggca ggcactttcc agtgcgccgc gtaaccagta
      901 caccgtcgag ctacacctgc aaatgattaa atatgccgac gaactggaac acattacggc
      961 aaaagccttt tgcgaaggca ccggtctgag ccagagcctc ggtaccgaat tcagcaaaat
     1021 gcgtaactta acccgtcggt tgaaggccgc cggtctgaat accgatctgc tgtaaccgac
     1081 ttacggcaga ttatcgcgca aaacgagata tttaatggct atcaaaaaaa ccgaactcta
     1141 ttcttccctg tgggcaagct gcgacgaatt gcgtggcggg atggacgcca gccagtacaa
     1201 agactatgta ctgaccctgc tgtttatgaa gtacgtttcc gataagtata aaggcgatcc
     1261 ttacgggatg atcgtgatac ccaaaggagc cagctttgac gatatggttg cgctgaaaaa
     1321 tgacaaagag atcggcgaca aaatcaacaa aatcatccac aagctggcgg aagagaacgg
     1381 cctgaagggc gtcatcgacg aagccgactt taacgacgaa gataagctcg gcaaaggcaa
     1441 ggaaatgatt gaccgcctga gcaagcttgt cggtatcttt gaagggttag atctctccag
     1501 caaccgggcc gaaggcgacg atctgttggg cgatgcctat gaatatctga tgcgtcactt
     1561 cgcaaccgaa tccggcaaga gtaaaggaca gttttacact ccggcggagg tgtcacgtat
     1621 tctggcgaag gtgattggta ttactcccga tacgccgcga gatgcaacgg tttatgaccc
     1681 aacctgtggc tctggttcgc tgctgttgaa agtgaatgat gaagcccgac gcggactgtc
     1741 gattttcggc caggagatgg ataacgctac cagtgcgctg gcgcggatga acatgattct
     1801 gcacaacaat gccaccgcga aaatctggca gggcaatacg ctgagcgatc cgcagtggaa
     1861 agaggcgaac ggcaaactga aagccttcga ttttgccgtg gcgaacccgc ctttttccaa
     1921 taaaaactgg accaacgggc tgacaccgaa aaaagatccg tttgaacgct tcggctgggg
     1981 tattccgccg gaaaaaaacg gcgattatgc ttttttgctg catatcatta aaagcctgaa
     2041 aagtaccggc aaaggcgcgg tgatcctgcc ccacggcgta ttgtttcgcg gtaacgccga
     2101 ggcaaatatc cgtgaaaacc taatcaagca gggctatatc aaaggcgtta ttggtttgcc
     2161 ggccaaccta ttttacggca ccagcatccc agcgtgcatt attgtgattg ataaagagca
     2221 cgcccacagc cgaaaaggca tttttatgat cgacgccagt cggggcttta tcaaagatgg
     2281 caacaagaac cgcctgcgta gccaagatat tcaccgcatt gttgacgtgt tcaaccatca
     2341 gcgaacggtg ccaggctaca gtcggatggt gccctccagc gagatcgctg ggaatgatta
     2401 caatctgaat attcctcgtt atatcgagag cggagatccg gaagatttgc acgatctgac
     2461 cgcacactta caaggtggta ttccggcgcg cgatgtagat gctttgaagg attactggcg
     2521 gatattccct gccctgcgta acgtgttatt tgctgacgac cgccccggtt actgccgggc
     2581 gcaagtggac gcgcagcagg tgaaaccgac cattctggcg catcaggagt ttaaggattt
     2641 tgccacccgc agcctgctac cgtttaagtc gtgggtgaaa gaggcaggtc tggaagagat
     2701 ccgcaaagga gataaaccca aagcgctgat ccacgatatc agcgaaatgc tattggcgca
     2761 gtacgccaac agtgagttgc tgaacaagta cagcgtgtac cagatcctga tggattactg
     2821 ggctaatgtc atgcaggacg atgtgtatgt gattatgcag gatggctggc aagccgccgc
     2881 gcaaatccgc gagcttcagc cggtgaaagg caaagatggc aaaaacatct ggaaagaaac
     2941 gcacgatttc gaatttacca aacggcgcta caagtccgat gtgctacccc gttcactggt
     3001 ggaaattcgc tgcttcccgg aactactgga tgcgctgaac cgtgcacagg aacgcagtga
     3061 cgaagccagc cgtcagttag cggaattcat cgaggagcag gcaggtgaag aggatctgct
     3121 ggccgaggcg aagaacgata aagataaggt gacgcaaaag ctggtgaatg cccgtctagc
     3181 acagttaaag aaaaccactg ccggcccaga tgagctggca gtgctgaacc gctgcctggc
     3241 gctaattaag gccgaagcca gtgcgaaaaa agcgctgaaa gccgcacaag aagcgctgga
     3301 tcgggcggtg tttaaacatt atcccacgct ggatgaagca gcgattaaaa cgctggtagt
     3361 gcaggataag tggctggcaa cgttgcaggc agggatcaag gcggagattg agcgtattac
     3421 ccagcaactg gcgtcgcgcg taaaggagct ggaagagcgt tatgctgaac cgttgctagc
     3481 gcttgaagcc tccgttgagg cgttgagcga gaaagtggcc gggcatttgc gggcgatggg
     3541 gctggagtgg tgatggtgga tttaatggtg aatgagcaac ggtcgggtac ggcggggaaa
     3601 gatgtgcctg cggggtataa actgactgag gtgggcgtga ttcctgagga ttgggattgc
     3661 gtaccatttg gtaatctgtt taaaacaaac acaaagaaaa aaaaagtcag cgattatgag
     3721 ctagtttcat ttattgggat gcaggatgtt agtgaagatg cacaattaaa aaataatact
     3781 caacttcctt ttaaagaagt taaaagtggt tttacttact ttgaaaaagg ggatgtttta
     3841 ttagctaaaa taaccccttg ttttgaaaat ggaaaaggat gtcataccgc tgatttacct
     3901 actaatgtag gttttgggag tacggaattt catgtattaa gagaaaatga agactccgat
     3961 tctcgcttca tctatttttg gacaactgat aaaaaattca gagcatcact ggaatctgaa
     4021 atggtgggaa gtgcgggaca ccgtcgtgtg ccgctagttg ctatagaaaa atatttgatt
     4081 ccatgtcccc caaatttgca aga
//
LOCUS       DAZFFG010000231         4050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFFG010000231 DAZFFG010000000
VERSION     DAZFFG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.232"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..810)
                     /locus_tag="ACHJ7J_004905"
     CDS             complement(<1..810)
                     /locus_tag="ACHJ7J_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5002997.1"
                     /translation="MRLASRFGYANQIRRDRPLAREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRSGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGNEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLES"
     gene            complement(899..1137)
                     /locus_tag="ACHJ7J_004906"
                     /pseudo
     CDS             complement(899..1137)
                     /locus_tag="ACHJ7J_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            complement(1143..1820)
                     /locus_tag="ACHJ7J_004907"
     CDS             complement(1143..1820)
                     /locus_tag="ACHJ7J_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5002998.1"
                     /translation="MNTRMLFPLLFTVASFSTSAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEAEQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARLAYARKYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            complement(1939..2823)
                     /locus_tag="ACHJ7J_004908"
     CDS             complement(1939..2823)
                     /locus_tag="ACHJ7J_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5002999.1"
                     /translation="MPFSNSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRALYRQLLPELDLIIWILRSDERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     MFPAEEWNATEKCPSRHQELSLATVTARVATLFPSSFPVLPVAAPAGWNLPALVSLMI
                     HALPPQATSAVYSHIRGENHSEQARKHAQQTFGETIGKSFDDAVARFSFPPWMLQLLR
                     KTRDRIIHLLVTLWDHLF"
     gene            2924..>4050
                     /locus_tag="ACHJ7J_004909"
     CDS             2924..>4050
                     /locus_tag="ACHJ7J_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003000.1"
                     /translation="MAKNKGICRNKKCSNPVSEFDEANALRLCDDHYKLWKRNKENRR
                     VRNICRIISKYDKSLDREIFYGVRDAAKNKTGKRIIEDAKILHQCRASKQVRKSFHKG
                     VLRSREWLNTQELLSRKHISVTGELFKFYIKDIIRLYCYKFLYSREYLNENAYKKRYQ
                     SDIHPRKVNVPILQLEVAWINNNCRSSDSSTDIIILPRMIRRMVVFNYTKINKQLEKL
                     LGGIARKQTSERNKGGLYTRIKKESDFLSMDRFMVEIENIIRPERFPTSIYPVFSHSF
                     IERELPLLNLARSQYKRAISKKVANSIFYTLQKTNVALWLHLEMFALVAFIKLYTGKG
                     FEFIKNMCAFIDYNNGIIKSTHEELLGEIKHNIQVNLKVNP"
BASE COUNT         1104 a          899 c         1020 g         1027 t
ORIGIN      
        1 gctctccagc agcgtttctg ccattaccca caatgcgcgg ttgagcttaa tatcggtgtc
       61 gatgctgtga atggcacggg tgtggatacg ttttcctttt gcactgcggc cggaaatccc
      121 gcctttcagc atattctcct gtatggtctg ataagcactc cacaggtcct taccgtaatc
      181 ctcccggcgt cgcggtgtca gaatatcagc ggtggtgacg ggctgatgct cattaccata
      241 acggtaagtc agtgccgcct gtgccagcgc ctggcgtgcc ggtggcggca gaatcagcga
      301 ctgcatggca tcacgcttct cctctatccg gtcaaaaacg cccaccacct cgtaagcccc
      361 ttcaataact ttctccacta cgtctccccg gtgtggcacc cggacttctc ccagcgactg
      421 accgcagacc aggccattgg tacaaatggc acgaaaatat ccgggtaaca tctggtagct
      481 ggatgaaccg tcatgggagt tgagcagaat aatttcaggc acatgctggc cggttatctg
      541 tccgctccgc cgcagacgca gcatgtgttt ggtatattcc cgacggctct ggtcgcgcac
      601 acgggtctgg caggcgaaga acggctgaaa gccttcccgc tgcaggtttt ccaggacggt
      661 gatggtggga atgtacgcat accgttcact gcgggaggtg tgccggtctt ctccaaaaat
      721 actcgggacg tggtacatca gttcttcgcg tgccaatgga cggtcacggc gtatctggtt
      781 cgcataacca aaacgacttg ctaatcgcat gatttgctcc ttatgaatga gttggcggta
      841 taaatgaaaa ggcgcgtctc ccggagaaga cgcggcctga tggatgaaat gaagactttt
      901 attgtctgag aagcccttca ctggcgagcc acacattaag ctgttttccg gcgtcagact
      961 caaaattcca gcaccgccag cgtagctgcc cttcggcatt gcggataacc agcaggaaat
     1021 gcgttccatg gtcatcttca ataagcacat tgcggtaagg taagctgttg tggcagccat
     1081 tgcctgtttg cgtgagaaag ggccttcggg caataaaaca gggtgacagt ccgacatacc
     1141 ggctattttc cgataaattc aacgctggca ctggtggcat taccgtcagc accaaccgtg
     1201 acggtcactt tgtcgtacgc acggtcttca ggattgaaca cttcaaactc aaccttattg
     1261 ccttttactt cgtaagagcc aaaatcagcg gtttctgttg tttcctcgtc gtatttccgg
     1321 gcgtatgcca gtcgggcagc ctgcagtacg gagtctttat taatagtggt cgtctgtttc
     1381 ggggaaaaac cggcaagtga tgtgacaaat ttcggacaga ccttcagcat atcaccgcgg
     1441 gtgaattcca ttcgtacacc aaatgcctgt aaaacggaca catcacgctc tgctatggag
     1501 tgaacggctt ttttgaccat ttccggcgcc tgttcggctt ccacctcaac agccctgaat
     1561 cccgtctgct gattacacat ttgtgatgtg gggatcaggt taccgttaat actgatacgg
     1621 ctgcccagga gcgtctgtac gccattcggg catccctgca tcaggccggt aataccaatc
     1681 gtgccatttt tcagcattac cagcgcctga ccatcttccg tcatggtctg gcaccagccg
     1741 tttttgacag cccagttacc ggcggaggtg gagaaggatg ccacagtgaa cagcagggga
     1801 aaaagcattc tggtattcat gactgacctc tgaaattaaa gagtgtgtta ttgataatga
     1861 cagacggtat acggcagtgg ttaccgtaat ccgttgtcgt gtaaaggtat acggttgcta
     1921 cgggggactc cggggtgctc agaacaggtg atcccacagt gtgaccagca ggtggataat
     1981 gcggtcccgg gttttgcgca gaagctgtaa catccagggc ggaaaactga aacgggcaac
     2041 ggcgtcgtca aaacttttcc cgatggtttc accaaaagtc tgttgtgcgt gtttccgggc
     2101 ctgttcagag tggttttccc cccggatatg tgaataaact gcgctggttg cctgtggtgg
     2161 cagcgcgtgg atcatcagtg acaccagcgc tggaaggttc cagcctgcag gcgcggctac
     2221 ggggagtacc ggaaatgatg aagggaacag ggtggccacc cgggctgtta ctgtcgccag
     2281 tgagagttcc tggtgacggg acgggcattt ttctgtggca ttccattctt cagcaggaaa
     2341 catgcgatcg gcatggctga gaacaaacag aaagcgcgag ggatctgcgc cctcattcag
     2401 taaaaactga tgcatggcaa tatcggcagc atacgcacgt tcatcactcc gcaggatcca
     2461 gataatcaga tccagttccg gcagtaactg acgataaagc gctcggtact cctgatcatg
     2521 ctgcggtgtt tcaccaatgc cgggcagatc gaccagcgtc attctgcgtt caccgagctg
     2581 cagggtaaga cgatgagcct ggcgggtgca gccgttcagg ggatgcgtgg cgcagatacg
     2641 ggactgaaaa atggcattac acaggctgct cttgccggtg ccggatttac ccataatgcc
     2701 aatcacgggc tcgtaatgaa taacaccgtg gagatgttca aggattttct ggcgtatttc
     2761 ttcaggaaga aacgtcagat atgatttcac ttgcgcggag agggaagaat tgctgaatgg
     2821 cataccggta tcctgttgat ttcactgaac cggatcacac taatccggtt tatcattcag
     2881 gtaatatggg tgtgaaaatt tttccattga cgggaagaag agtatggcta aaaacaaagg
     2941 tatttgtcgg aataaaaaat gcagtaatcc tgtttcagaa tttgatgagg caaatgcctt
     3001 aaggttatgc gatgatcatt ataagctatg gaagcgaaat aaagagaatc gtcgggtcag
     3061 gaatatttgt cgtataatca gtaaatatga caaaagtctg gaccgggaga tattttatgg
     3121 agtaagagat gctgcaaaaa ataaaacggg aaagagaatt attgaggacg caaaaatatt
     3181 acatcaatgc cgtgcctcaa agcaagtaag aaaaagtttt cacaagggtg tgcttcggtc
     3241 acgggaatgg cttaatacac aggagttact gagcagaaaa catatatctg ttacaggtga
     3301 actatttaaa ttttacataa aagatattat cagattatat tgctataagt ttctctacag
     3361 cagagaatat cttaatgaaa acgcatacaa aaaacgatat cagagtgata ttcaccccag
     3421 aaaagttaat gttccgatac tgcaacttga agttgcatgg ataaataata attgccgttc
     3481 aagtgattca agcactgata ttattatcct tccgaggatg ataagaagaa tggttgtatt
     3541 taattatacg aagataaata aacaattaga gaaattgtta ggggggatag cacgaaaaca
     3601 aacctcagaa aggaataaag gcggtctcta taccagaata aaaaaagaaa gtgactttct
     3661 ttctatggat agatttatgg tggaaataga aaatatcata aggcctgaaa ggtttccgac
     3721 atcaatttat ccagtctttt cacattcatt tattgaacga gagcttccgc tactgaatct
     3781 tgctcgatca cagtataaaa gagcaataag taaaaaagtg gcgaattcaa ttttttatac
     3841 tttgcaaaaa actaacgtag cattatggct acatcttgaa atgtttgctc tggtggcatt
     3901 cattaaactg tatacaggaa aaggttttga attcataaaa aacatgtgtg catttatcga
     3961 ttataataat gggataataa aatccacaca tgaagaacta ctgggtgaaa ttaagcataa
     4021 cattcaggtt aatttgaagg tgaaccctga
//
LOCUS       DAZFFG010000232         4027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFFG010000232 DAZFFG010000000
VERSION     DAZFFG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.233"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            84..692
                     /locus_tag="ACHJ7J_004910"
     CDS             84..692
                     /locus_tag="ACHJ7J_004910"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO5003001.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLQVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            790..2181
                     /gene="selA"
                     /locus_tag="ACHJ7J_004911"
     CDS             790..2181
                     /gene="selA"
                     /locus_tag="ACHJ7J_004911"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO5003002.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2178..4022
                     /gene="selB"
                     /locus_tag="ACHJ7J_004912"
     CDS             2178..4022
                     /gene="selB"
                     /locus_tag="ACHJ7J_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO5003003.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDVLREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          898 a         1026 c         1204 g          899 t
ORIGIN      
        1 gaggtaacaa accatcaaca ttttctgccc tttttcccat caccaggcca tactgatatt
       61 tttcctcgca caaaggactc ggcatgaaac tcgtcggtag ctacaccagc ccgtttgtac
      121 gcaaactttc tattctgttg ttagaaaagg gtataacttt cgaatttatt aatgaactgc
      181 cctataacgc ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg ccggtgctgg
      241 tgaccgaaga gggcgaatgc tggtttgatt cgccgatcat cgctgaatat attgaattaa
      301 tgaatgtcgc tccggcgatg ttgccgcgcg atccgctgga gtcgttgcag gtgcgcaaaa
      361 ttgaggcact ggcggatggc attatggatg ctgggctggt atcggtgcgt gaacaggcgc
      421 gtccagcggc gcagcagtct gaagatgaat tgttacgcca gcgggagaaa atcaaccgca
      481 gtctggatgt gctggaagga tatctggtcg atggcacact caaaaccgat acggtcaatc
      541 tggcgactat cgccattgcc tgtgccgtcg ggtatctcaa tttccgccgc gttgcgccag
      601 gctggtgtgt cgaccgccca cacttagtca aactggtcga aaacctgttt agccgcgaaa
      661 gttttgctcg caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct gacccatctc
      721 acgttacaat ccgtggttat gttaaacgcc cttctccgtg tgagagggcc ttgatcagcc
      781 aggtttccta tgacaaccga aacgcgttcc ctctatagtc aacttccggc tattgatcgc
      841 ttattgcgcg atagctcctt cctttctttg cgtgatactt atggtcacac ccgcgtggtg
      901 gaattgttgc gtcagatgct cgacgaagcg cgagaagtga ttcgtggcag ccagacgctg
      961 cctgcgtggt gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa agaggcgcag
     1021 agtgcgctgc gtccggtgat caacctgacg ggaaccgtgc tgcataccaa ccttgggcga
     1081 gctttacagg cggaagctgc ggtggaagcc gttgcgcagg ctatgcgttc gccagtgacc
     1141 ctcgagtacg atttggacga cgccggacgc ggacatcgcg atcgggcgct ggcgcagctg
     1201 ctgtgccgca ttaccggcgc ggaagatgcc tgtatcgtta ataacaatgc ggcggcggtg
     1261 ttattgatgt tggcggccac tgccagcggg aaagaggtgg tggtttctcg cggcgaattg
     1321 gtggagattg gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg ctgcacacta
     1381 cacgaagtag ggaccaccaa ccgcacgcac gcgaatgatt atcgtcaggc ggtgaatgaa
     1441 aataccgcac tgttgatgaa agtacatacc agtaactaca gcattcaggg gttcaccaaa
     1501 gcgatagacg aagctgaact ggtggcgctc ggcaaagagc tggatgttcc agtggtgact
     1561 gatttaggca gtggctcgct ggtcgatctt agccagtacg gtttgccgaa agagccaatg
     1621 ccgcaggagt tgattgcggc gggcgtcagt ctggtgagct tctccggcga caagttgtta
     1681 ggcgggccgc aggcagggat tatcgttggt aaaaaagaga tgatcgcccg cctgcaaagc
     1741 cacccgctga agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct ggaagccacg
     1801 ttgcgtcttt atttacaccc tgaagctctg agtgaaaaat taccgaccct gcgcctgctt
     1861 acccgcagcg cagaggtcat tcaaatccag gcacaacgtt tacaggctcc ccttgccgca
     1921 cattacggcg cggagtttgc ggtacaggtt atgccatgtc tttcgcagat tggcagtggt
     1981 tcgctgccgg ttgatcgcct gccgagcgcg gcattaacgt ttacacccca tgatggacgc
     2041 ggtagccacc ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc ggtgattggt
     2101 cgtatttatg acggacgatt gtggctggat ttacgctgcc ttgaagatga gcaacggttt
     2161 ttggagatgt tgttgaaatg attattgcga ctgccggaca cgttgaccac ggcaaaacaa
     2221 ccttattgca ggcgattact ggcgtaaatg ccgatcgtct gccggaagaa aaaaagcgcg
     2281 gcatgactat cgatctcggc tatgcctact ggccgcagcc ggatggccgc gtgcctggtt
     2341 ttatcgacgt acccggtcat gaaaagtttc tttccaacat gctggcgggc gttggtggta
     2401 tcgatcacgc gctgttggtg gtggcgtgcg atgacggcgt gatggcacag acccgtgagc
     2461 atctggcgat tttgcagctg accggtaacc cgatgctgac agtggcgctg accaaagccg
     2521 atcgcgtgga cgaagcgcgt gttaatgagg ttgaacgcca ggtaaaggag gtgctgcggg
     2581 aatacggttt tgctgaggca aaactgttta tcaccgcagc aaccgaaggt cggggaattg
     2641 atgtcctgcg cgagcatctg cttcagttac cagaacgtga acacgccagc caacatagtt
     2701 tccgcctcgc gatcgaccgc gcatttaccg taaaaggtgc cgggctggtc gtcaccggta
     2761 cggcgttaag cggggaagtg aaggtaggcg attcactatg gctgactggt gtaaataaac
     2821 cgatgcgtgt acgtgcgctg catgcacaaa accagccaac agaaaccgct catgccgggc
     2881 agcgtatcgc gcttaacatc gcgggggatg cggaaaaaga gcagattaac cgtggcgact
     2941 ggctgcttgc cgatgtgccg ccagagccgt tcacgcgggt gattgtcgag cttcaaaccc
     3001 atacgccgct gacccagtgg cagccgctgc atattcacca cgccgccagc cacgttacgg
     3061 gacgcgtttc gctgctggaa gataaccttg ccgagctggt gttcgacacg ccgctatggc
     3121 tggcggataa cgatcgctta gtgttgcgcg atatctcggc ccgcaatacg ctggcggggg
     3181 cgcgtgtggt gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa tatctgcaat
     3241 ggctggcgtc tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt catctggaac
     3301 gcggagcggt taaccttgcg gatttcgcct gggcgcgcca gctcaacggc gaagggatgc
     3361 gcgaactgct gcaacagccg ggctatattc aggctggtta tagcttgttg aatgcgccag
     3421 ttgccgcccg ctggcagcgg aaaattcttg acacattagc gacttatcat gagcaacatc
     3481 gcgatgaacc tggtcctggg cgcgaacgtc tgcgacgtat ggcgttgcca atggaagatg
     3541 aagcgctggt actgctgctg attgaaaaaa tgcgcgaaag cggcgacatc cacagccatc
     3601 acggctggct gcatctgcca gatcacaaag cgggcttcag cgaagagcag caggccatct
     3661 ggcaaaaagc agagccgctg tttggtgacg aaccgtggtg ggtgcgtgac ctggcaaaag
     3721 agacgggaac cgacgagcag gcaatgcgtc tgacgctacg ccaggcggcc caacagggga
     3781 taattaccgc gatcgtcaaa gatcgttatt accgtaacga tcggattgtc gagtttgcca
     3841 atatgatccg cgatctcgat caggaatgtg gttcaacctg cgcggcggat tttcgcgatc
     3901 gcttaggggt tgggcgaaaa ctggcgattc aaatcctgga atattttgac cgcattggct
     3961 ttacgcggcg tcgcggaaat gatcatttat tgcgcgacgc attattattt ccggaaaaat
     4021 aaggaaa
//
LOCUS       DAZFFG010000233         3991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFFG010000233 DAZFFG010000000
VERSION     DAZFFG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.234"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            85..1728
                     /locus_tag="ACHJ7J_004913"
     CDS             85..1728
                     /locus_tag="ACHJ7J_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR-like domain-containing protein"
                     /protein_id="HFO5003004.1"
                     /translation="METQVYINSREASSKASDGVSKAVFPDPCWTPPSPPAGPIVVPY
                     PNTAYASTMKDGTITVFICNSMVAKEDISFFSTSTGDEPATQSQPKGVITGVIKGKAY
                     FKSWSPNVKFEGKCVARHEDLMTHNHGSEPGNTITGTYKDTLDMPVQCKKEEDRIRKA
                     CKPDEDNKNDDEDEKKKEENRRNHVKNKNFMKLMGKEIGGKLETLDDKVRNTYAKLKT
                     PENEWIFSHCTGLWIKPRFGLEEYNKLLKELTEDFNKKVASLIEPMLEQLKQEIIDKA
                     QTAALHKAEKLAAHAAGRWAVGTIGATAAGVGAVVTETIATIWNVCDAIGTAYDTAKL
                     GIEAYKKLDEIKDILSLVKQAKAELENTLKNMTPSQMMATGMGALARINPCTRARRCL
                     LVDYNKTGAKKSIETGKGCCPGQTGHHLIPDEMAKGHCSGYSKGGAPTVCVEGTNNGN
                     GTHGKMHDEMIKDIKAYKKSGWFSDKDKIDYEDAKEIALDGFERTFPESRCSRACLRK
                     QLDNYYKNCDDDMPAVAGKAFTEDEVDSQNTETPTGNKR"
     gene            1736..2764
                     /locus_tag="ACHJ7J_004914"
     CDS             1736..2764
                     /locus_tag="ACHJ7J_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFO5003005.1"
                     /translation="MKITDKNIAIFKKWYIKDCTVRNKDFFHFSVRNIKESRKASVID
                     ENTVTKGEVGIYLDGSPDDCISLQPLKNMGQLYIGSASYNSYDEVVCVDDEGYVYSRS
                     CKYDSREKKIPFGIDGPLRNIITKIKNISGRLYIVGYNNSVGYRDGVNDWQSLCLNLP
                     VFDYSKEKGRFGDEFKLRDIDGFSYDDLYVVGGKGNVWHFSGAEWEQINFPSNIYLET
                     VCCAGDGYVYIGGQSGILFKGRGNKWEKVSAGGYSLPFEDIVWHAGKVWCTSDYGLWC
                     LDGDTLIPVDLPSEIAVAVGHLSVGDGVMLMAGMYGAAWHDGQEWHLLINSVEWQSDD
                     ETDDEAED"
     gene            2766..>3991
                     /locus_tag="ACHJ7J_004915"
     CDS             2766..>3991
                     /locus_tag="ACHJ7J_004915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003006.1"
                     /translation="MNHQVIQPIIRQHAANAAFYWLQKEESRFSPLVKSADLRQFNQY
                     IDANLEGLHVAGDDGWEESYAALRRWHSQGEAFVCGFLANQCQNPAWMKATWSYVSKY
                     ADTTLNGYVSALSHSAKEHVYGTVNEFLLSSIPSEVQIALNICSQSKLHLSEDFLIKK
                     LDNGNIQEKVAVLDYIRKLGLQSYLPALTVYFGSNELSVRFSAVSAACWLSSDKSMMI
                     NPLCLLIDDYLTLPPLRGIEGIRKNQQTEMLVRLLGQCCSELTYPFAFISGLPEYLQI
                     IFYAHYANTKTLPLLLSLMEKEELSRIAFVAVSLITGIDIDDKEYLLPAKDEKLPEIT
                     SRLNVFGTGIGMPDIHKVTSMCQQYRNEERLFLGKSVTASWCNTNFSDGSQLVRWIAS
                     WHLFHLDKNTSPKDNLL"
BASE COUNT         1301 a          645 c          928 g         1117 t
ORIGIN      
        1 taactatgcc ggtaataaat aattcataaa tatactgatt gccgtatcca cgggaaatta
       61 cgataagaaa gatgaatatc taatatggaa actcaagttt atatcaatag ccgggaggca
      121 agctccaagg catcggacgg cgtgtcaaaa gcggtgtttc ccgatccgtg ctggacaccg
      181 ccttctcctc cggctggccc gatagtggta ccttatccaa atactgcata tgcctcaacg
      241 atgaaggacg gaaccataac cgtattcata tgcaattcga tggtggctaa agaagatatt
      301 tccttttttt caaccagtac cggagatgaa cccgctacac aatcccaacc caaaggtgta
      361 attaccggcg tcattaaagg aaaagcctac tttaaatcat ggtcgccaaa tgtaaaattc
      421 gaagggaagt gtgtggcacg ccatgaagat ttaatgacgc acaatcatgg ttcggagcca
      481 ggaaatacta ttacaggcac atataaggat actcttgata tgcctgtaca gtgtaaaaag
      541 gaagaagatc ggataaggaa ggcatgtaaa ccggatgaag ataacaaaaa tgatgatgaa
      601 gatgaaaaaa agaaagagga aaacagaagg aatcatgtaa aaaataaaaa cttcatgaaa
      661 ttaatgggta aagaaattgg tgggaaactg gaaacgctgg atgataaagt cagaaataca
      721 tacgctaaac taaaaactcc ggagaatgaa tggatttttt ctcattgtac tggtttgtgg
      781 attaagccac gttttggcct ggaggagtat aacaaattat tgaaagaact gacggaggat
      841 tttaataaaa aagtggcttc actaatagag ccaatgcttg agcaactaaa acaagaaatt
      901 attgataaag cacaaactgc tgctctgcat aaagcagaaa aactagcagc acacgcggct
      961 ggaaggtggg ccgtgggtac gataggtgct acggctgctg gcgttggagc agttgtaacc
     1021 gagacaattg caacaatatg gaacgtctgt gatgcaattg gtacagctta tgatacagcc
     1081 aagttaggta ttgaagcgta taaaaaatta gatgagataa aagatatttt atctttagtc
     1141 aaacaggcca aagctgaact ggaaaatacg ctaaaaaata tgacgccatc tcaaatgatg
     1201 gcaacaggaa tgggggcgct ggcgagaata aatccctgta cgcgtgcaag acgatgcctt
     1261 ctcgttgatt ataataagac cggagccaaa aagagtatcg aaactggaaa agggtgctgt
     1321 cccggacaga caggccacca tcttattcct gatgaaatgg ctaaaggaca ttgcagtggt
     1381 tattcgaaag gcggggcacc gacagtttgt gtagagggca caaataatgg aaacggaacg
     1441 catggcaaaa tgcatgatga gatgatcaaa gatatcaaag cttataagaa aagtggctgg
     1501 ttcagcgata aagataaaat tgattacgaa gatgccaaag aaattgccct ggatggattt
     1561 gaacgaacat tcccggaatc aagatgcagc agagcctgcc ttagaaaaca actggataat
     1621 tattataaga attgcgatga tgatatgcca gcggtagctg gaaaagcatt tactgaagat
     1681 gaagttgatt ctcagaatac tgaaacacct acaggtaata agaggtaata tttatatgaa
     1741 aattactgat aagaacattg caatttttaa aaaatggtat attaaggatt gtactgtaag
     1801 aaataaggat tttttccatt tttctgttcg taatattaaa gagagtcgaa aagcaagtgt
     1861 tattgatgaa aatacagtaa ctaaaggtga agtcgggatc tatttagatg gtagcccaga
     1921 cgattgtata tcacttcagc cacttaaaaa catggggcag ttatatatag gtagtgcatc
     1981 atataatagc tatgacgaag ttgtttgtgt tgatgatgag ggatatgttt attcccgtag
     2041 ttgtaaatat gatagcagag agaaaaaaat tcctttcgga attgacggtc ctctaagaaa
     2101 tataataacg aaaattaaaa atatttcagg aagactgtat attgttgggt acaataactc
     2161 tgtgggatac agagacggag ttaatgactg gcagtcactc tgtttgaatc tcccggtttt
     2221 tgattactca aaagaaaaag gtagatttgg cgatgaattt aaactaagag atatagatgg
     2281 tttttcctat gatgatttgt atgtagtcgg cggtaaggga aatgtctggc atttttctgg
     2341 agcagaatgg gagcaaatta attttccctc aaacatctat ctagaaacag tgtgttgtgc
     2401 aggggatggt tatgtctaca ttggtggtca atctggtata ttatttaaag gccgtgggaa
     2461 taaatgggaa aaagtctctg cgggtggtta tagtcttccc ttcgaagata tagtctggca
     2521 tgccggaaaa gtgtggtgta catctgatta cggtctatgg tgtctggatg gtgatacatt
     2581 aattcctgtg gatcttcctt ctgaaatagc tgtagcggta gggcatttat cagtgggtga
     2641 tggtgtgatg ctaatggccg gaatgtatgg tgcagcatgg cacgacggac aggaatggca
     2701 tttactcatt aattctgttg aatggcagtc agatgatgag actgatgatg aggcagaaga
     2761 ctagtatgaa tcatcaggta atacaaccga ttattcgtca gcatgccgcg aatgcggcat
     2821 tttactggct tcagaaagaa gaaagcagat tttcgccatt agttaaaagc gccgatctca
     2881 ggcaatttaa tcaatatatt gatgctaatc tggagggatt acatgttgct ggtgatgatg
     2941 gatgggagga gagctatgcg gcactgagac gctggcactc tcagggagaa gcgtttgttt
     3001 gcggttttct ggcgaatcag tgtcagaatc ctgcctggat gaaagcaacc tggtcgtatg
     3061 tatctaaata tgcagataca acgttgaatg ggtatgtctc tgcgttatca cattcagcta
     3121 aagaacatgt ttatggtact gttaatgaat tcttactttc ctctatacct tccgaggttc
     3181 agatagcatt aaatatttgt tctcagagca aattacactt atcagaagat tttttgataa
     3241 aaaaactaga taacggaaat atacaagaaa aggtagctgt gctggattat attcgcaaac
     3301 ttggattaca gagctatctt cctgcgttaa cggtatattt tggtagcaat gaattatcag
     3361 tcagattttc agcagttagt gctgcatgtt ggctttcatc agataagtca atgatgatta
     3421 atccattatg tctgttgatt gatgattatt taaccctgcc gcctcttaga ggcattgaag
     3481 gtatcaggaa aaaccagcaa actgagatgt tagttcgact tcttgggcaa tgttgttctg
     3541 aactgactta cccttttgca tttatttctg gtttacctga gtatttacaa atcatttttt
     3601 atgcccatta tgcaaataca aaaactctac ctctattact ttcattaatg gaaaaagaag
     3661 agctttctcg tattgcgttt gttgctgtaa gtctgataac tggcattgat atagatgata
     3721 aggaatattt attaccggca aaggatgaaa agttgcctga gattacctct cggttaaatg
     3781 tgtttggaac cggcatagga atgcctgata ttcataaagt tacatcgatg tgtcaacagt
     3841 atcgtaatga ggaaagactg ttcctgggga aatcagtgac tgcatcttgg tgtaatacta
     3901 atttttctga cggaagccaa ctcgttcgct ggattgcttc atggcatctt ttccatttgg
     3961 acaagaatac ctcaccaaag gataatttgc t
//
LOCUS       DAZFFG010000234         3986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFFG010000234 DAZFFG010000000
VERSION     DAZFFG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.235"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            58..3810
                     /locus_tag="ACHJ7J_004916"
     CDS             58..3810
                     /locus_tag="ACHJ7J_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO5003007.1"
                     /translation="MGDVKVTLSAMIACAVLLHPNKLFASVAGTSLPYQTYRDFAENK
                     GEFRPGAEDVPLYDKNGNPVMTLNKAPMIDFSSNDSIGVATLVSPQYVVSVKHNGGYQ
                     YVKFGYADDSSYALVDRNNHWRDFHTPRLNKIVTEVTPLDITNAGTAKGTYQNADRFP
                     VFYRVGTGTQYVKDTNGKITYLMGAYSYKTGGLVNTPAISDWSFVTNTTNTPLSTYGT
                     PGDSGSPLFAWDADQNKWVLLAVLNSYAGVNGNTNWYTIIPAGDVKNTMKQDADAPVN
                     TKQGEGDIHWSYDEKTGLGSLTQGSTSWAMHGNLEATWPASLNSGKDLTFQGGGTVVL
                     ENTVNQGAGTLTFNDDYIVKPVDTQAWKGGGVIVNGEHLVDWQINGVTGDSLHKLGTG
                     TLKINGTGVNPGSLSVGDGTVILAQRADDNGLSQAFSSVSIVSGRPTLVLNDDKQINP
                     DNIKWGYHGGKLDINGNSLTFHKLNGADNGAILTNSGSMANVNLDFNSPDTTATIANI
                     WHGHFTGNLNINNEVTAGTQNDFAIDGGVNTQGSITQQNGRLFMQGHPVVHAVSSQDV
                     ANKLKALGDNSVLTQPVSFTQNDWENRQFSMAELNLQNAEFNLARNASLNTRINADYS
                     TVTLGSEDLYIDLNDGNGVATKPTLGKSKATAEDDQSRFNGHVQLKQGSTLTINEHFV
                     GGIDSTDSATTITSTDTTLNQLSRFTQSSLSLGEGAKLTATAGLLSDGTVSSNAGAGL
                     SLLSDQPGTMYSAQSWELSGQDTSLNVGAGGIITGDINANDAASIIFGTTDINQSTNY
                     YGNINAPLASVTMKDTAWQANKQSVVKSLTLNGSTLSFNRLGQGGLTADTLEATNSSF
                     IINADGKAADTVTVNQALTGGNNTLVVIPTTNSIKQGGDPVSLVTAPKNTQSDIFTLN
                     PVSINAGFHSFTPQLDVLETDVNKQWRLEGFYIQPDKAALRTGKSFMDLGYKNFITEI
                     NNLNDRMGDLRHTHGETGAWARLNSGSGSATDGFTGSYTHLQIGADRKHIIESGELFT
                     GVTATFTSSNNRGTGWSGRTKSTGIGVYASAMFDSGLYVDTIGKYVRHNNYYSSSALG
                     MPEQDYGSHSWYLGAEAGWRFSLPDETYIQPQTELIYGTVSENQFAWQFNGGEVYMQR
                     KQMQPLIGRTGIEFGKTFSGKDWEMTALTGVNYQYDLFKPTVTAFKDLAGDTYIDNGK
                     DSRVVFNVGLNTKIKENTRISLNVERSEFGSYNIDKLINANIRYTF"
BASE COUNT         1193 a          766 c          946 g         1081 t
ORIGIN      
        1 aaaattacag tccttaatca gcgatataaa aaacattttg gttatcctca tattctgatg
       61 ggtgatgtaa aagtaactct ttcagcaatg attgcttgcg ctgttttatt acacccaaat
      121 aaattgtttg cctccgtcgc tggaacctca cttccctatc aaacatatcg tgattttgct
      181 gagaataagg gagagttccg ccccggcgcg gaagatgtac ctctatatga taaaaatggc
      241 aatcccgtca tgacgctaaa taaagcgcca atgattgact tcagctctaa tgactcgatt
      301 ggggttgcaa cattggtttc gccacaatat gtcgtcagtg ttaagcacaa tggtggttat
      361 caatatgtga aatttggcta tgcggatgat tcctcctatg cccttgttga cagaaataat
      421 cactggagag atttccacac tccccgtctc aacaaaattg tcaccgaggt tacaccgctg
      481 gatataacga atgcgggcac agctaaggga acctatcaaa atgctgaccg tttccctgtg
      541 ttctatcgag taggtacggg aactcaatat gtaaaagata ctaacggaaa aatcacctat
      601 ttaatggggg cctactcgta taaaacaggt ggattagtaa atacgccggc tatatctgac
      661 tggagttttg tcacgaatac cactaatact ccactctcaa cctacggtac tcccggtgac
      721 agcggctctc ctctttttgc ttgggatgca gaccaaaata aatgggtttt gttagcagtt
      781 ctcaattcat atgcaggagt caatggcaat actaactggt acacaataat tcctgccggt
      841 gatgtaaaaa atacgatgaa acaagatgcc gatgcaccgg taaatacaaa acaaggtgaa
      901 ggggatattc attggtcata tgacgaaaaa acggggcttg gttcattaac ccagggaagc
      961 acatcatggg caatgcatgg taatttggaa gcaacatggc ctgcttcgct taatagtggt
     1021 aaagatctaa cttttcaggg cggcggtact gtagtgttag aaaataccgt taatcagggg
     1081 gctggcacac ttacttttaa tgatgattat attgtcaagc ccgttgatac tcaggcatgg
     1141 aaaggcggcg gagttattgt taatggtgag catcttgtcg actggcagat taatggggtt
     1201 actggtgata gtttgcataa actgggtacg ggtacgttaa aaattaacgg taccggtgta
     1261 aacccaggaa gcttaagtgt gggagacgga accgttatat tggcacagcg tgctgatgat
     1321 aacggactga gccaggcatt cagttctgtc agtattgtaa gtgggcgtcc tactctggtg
     1381 ctaaacgacg acaaacaaat taatccggat aatatcaaat ggggctatca tggcggcaag
     1441 ctcgacatta atggaaacag tttgactttt cataaactta acggggctga taatggagct
     1501 atactgacta acagtggctc catggctaat gttaatctgg atttcaacag cccagataca
     1561 acagccacga ttgctaatat ctggcacggt catttcaccg gtaatctaaa tatcaataat
     1621 gaggttacag caggaacaca gaatgatttc gctattgatg gtggagtgaa tactcagggg
     1681 agtataaccc agcaaaatgg tcggctattt atgcaaggac atccggttgt tcacgccgtc
     1741 agcagccagg acgtagcaaa taagttaaag gcgctgggtg acaattcagt actgacccaa
     1801 ccagtgtcat ttactcagaa cgattgggaa aatcgacagt tcagtatggc ggagcttaac
     1861 ttacaaaatg ctgaatttaa ccttgcgaga aacgccagcc ttaataccag aattaatgca
     1921 gattattcta ccgtgacatt aggtagtgag gatctttaca ttgatctcaa tgacggcaat
     1981 ggcgtggcaa ccaaaccaac attgggtaag tcaaaggcaa ccgcagaaga tgatcagagt
     2041 cgtttcaatg gacatgtaca gttgaaacaa ggatcgacac taactattaa tgaacatttt
     2101 gtcggtggta ttgatagtac agatagtgcg acgacgataa cctctacaga taccacactt
     2161 aatcagctca gtcgtttcac tcaatcttct ttgtcattag gtgaaggggc aaaattgacc
     2221 gctacagccg gattactttc tgacgggacg gtaagcagta atgccggggc gggcctttcc
     2281 ttgctatcag accagcctgg aacgatgtat tccgcccaaa gctgggagct tagcgggcaa
     2341 gacacgagtt taaatgttgg cgcgggtggg atcatcactg gagatatcaa tgccaatgat
     2401 gcggccagca ttatctttgg cacgactgat attaaccaat ccacaaatta ctatggaaat
     2461 atcaacgcgc cgttggcaag tgtaacaatg aaggacactg cctggcaggc aaacaagcag
     2521 tcggtggtta agtcgctcac tttgaatggt tcaacactga gttttaatcg tctcgggcag
     2581 ggggggctga cggcagatac gctggaggcc actaacagtt cgttcattat caatgccgat
     2641 ggtaaagcag cagatacggt gacggtaaac caggcattga caggggggaa taacacactg
     2701 gttgttattc ctacgacaaa ttcaataaaa cagggcggtg accctgtgtc attggttact
     2761 gccccgaaaa atacacaaag cgatatattt acacttaacc ctgtttcaat aaatgcaggg
     2821 ttccatagtt tcacaccgca acttgatgtg ttggaaaccg acgttaataa gcaatggcgt
     2881 ctggagggat tttatataca gcctgataaa gccgctttgc ggacaggcaa gagttttatg
     2941 gatctcggtt ataaaaactt cattacagaa attaataatc tgaatgatcg tatgggtgac
     3001 ttacgtcata cccatggtga aactggggca tgggctaggc ttaacagtgg ttcgggctca
     3061 gctactgatg gatttaccgg aagttatacg cacttgcaaa ttggggcaga caggaaacat
     3121 ataattgaaa gcggagagtt atttactgga gtaacggcga catttacaag cagtaataac
     3181 cggggaactg gttggtcagg aagaaccaaa tcaacgggta ttggcgtgta cgcttcagct
     3241 atgtttgact ctggacttta tgtagatact atcgggaaat atgtgcgtca taacaattat
     3301 tactcatcaa gtgcgttagg aatgccagaa caagattatg gctcgcactc ttggtatttg
     3361 ggcgctgagg ccggttggcg tttttcctta cctgacgaaa catatataca gccgcaaaca
     3421 gagttgatct acggtactgt atctgagaat cagtttgcct ggcaattcaa tgggggtgag
     3481 gtctatatgc aacgtaagca gatgcaacca ttgattggtc gcacaggaat tgaatttggc
     3541 aaaacattca gcggcaaaga ctgggaaatg acagcattga ctggggttaa ttatcagtac
     3601 gatttattta agcctactgt gacagcattc aaggatctgg caggtgatac atatattgat
     3661 aatggtaaag atagcagagt ggtattcaat gtcgggctaa atactaaaat taaagaaaac
     3721 actaggataa gtctgaatgt ggagcgttct gagttcggtt cttacaatat cgataagcta
     3781 ataaatgcta atattcgtta tacattctga gtaggagagt agataaaaaa gaaaacctga
     3841 acatattgtt caggttttct tattgattta ttgttgtagc tagtcaaata tgcttatcta
     3901 ttggatattc tgaagtgtaa aattttatct tatgctgcta ttttttaggg tggctttatt
     3961 ctttacttta gcttatagtt ggattc
//
LOCUS       DAZFFG010000235         3920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFFG010000235 DAZFFG010000000
VERSION     DAZFFG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.236"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            20..532
                     /locus_tag="ACHJ7J_004917"
     CDS             20..532
                     /locus_tag="ACHJ7J_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5003008.1"
                     /translation="MISTAHSASTGTITFNGELTANTCDAVVDNQNADAIVTLPTVGI
                     NQLENQGQTAGRTGFNIALTNCASALTAASAFFAAGPSVDFASGHLKNTDESDGGAKL
                     VSLQLRDEAPGAGSKIIQAGNGNQATSTQYVDVISGTANLPYSVEYFADGTATAGNVT
                     SNVVYFIQYK"
     gene            634..1311
                     /locus_tag="ACHJ7J_004918"
     CDS             634..1311
                     /locus_tag="ACHJ7J_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5003009.1"
                     /translation="MKSLPLLFVTLFSLHSEASIVITGTRVIYPAGEHSVSVKLNNNG
                     KLPVLIQSWVDNGDPDKKPDDSQAPFIITPPINRVDPGKGQTLRVTFTDLPLPTDRES
                     VFWLNVLEIPTRYEPKSNDNYLQVAFRTRIKLFYRPENLPGKSLEAPNTLIWKISSGQ
                     LMASNNSAWNVSLSSISVCGKKVEGKMVSPRGSEIFPLKVSPGMRVSFEAVNDYGAIM
                     KKTATVQ"
     gene            1336..>3920
                     /locus_tag="ACHJ7J_004919"
     CDS             1336..>3920
                     /locus_tag="ACHJ7J_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5003010.1"
                     /translation="MENRIFKPHIIAVTIAGLYGFSVHAVFANDTINSSDSPVAQENT
                     KKSLPSPTSERIEFATEVLELKNKQSIDLSRFSSGNYSLPGKYQAGIWINDSFLGNQD
                     IEVKGHTDGSSYVCITPDLLKILPLSWDRLSEYKFNQAPESTNCSDVLASIPDSRVTF
                     DSGEQRLDVAIPQKFIIHQARGSVSPDLWDSGIPAAFLSYNMNSWASQSRGYDYKSLY
                     TSVNSGMNIGAWYFRHNGSYTWSESGEKDYQSINTYAQRDILSIKGRLLIGQSNTTGR
                     LFDTVPFSGVQLASDERMYPSSQRGYAPEIHGIAKTHAKVTVRQNSVVIYETTVSPGE
                     FVIDDLYPTGFGGDLMVNIHEADGQERYFTVPFASTPQLLRPGSYRYSFTGGKLRQIS
                     LSEKPQFYEATWQHGLTNFLSGYLGWQQSENYYAAQAGLAVGSLAGSLSFDITQSHAT
                     FDKSGRNTGPLSGNDSSSSGQSYRLSYSKLIQNTGTSINLAAYRFSTEGYYDFTTAMM
                     TREAIKRGYDSNVIKRAKSRYTLSVNQSLAESLGQLYASASLQKYWNSDDSDKQYQLG
                     YSNHRKLVSYGISVSRSYSWYGKSDTSWMASVSFPLGNVSEEHTPNIRFDAVRTSGGH
                     YSESASISGNAGVENQYSYGVTASNAGGDTGNSLFVNGQWRTPYTYLSGSNSNGRNYR
                     SQSLGASGSLIAHSGGVTLSPYISDTWALVEAKGAKGARVSSYPGVMVDRFGYAAVPY
                     LNAYELNEVAIDPNNADNDVGFENTSRKVAPYSGAIVKVKYDTQTGVPVLIDAGWKGQ
                     PIPFGADVIDESGNYMATAGQAGQIFTRLEPGRHVLKLKAGSQGSCSLPVEIPETVSP
                     SNLLR"
BASE COUNT         1130 a          850 c          895 g         1045 t
ORIGIN      
        1 tcttgtagca accgttggaa ttatcagtac agcccactcg gcttcaaccg gtacaatcac
       61 atttaatggc gaattgactg ctaatacctg tgatgcggtt gtagataacc agaatgcaga
      121 tgcaatagtt acgttaccta ctgttggtat taatcaactg gaaaatcagg gtcagactgc
      181 tggtcgtaca ggctttaata tcgcactgac taactgtgcc agtgcgctga cagctgcttc
      241 cgctttcttt gccgctggcc ccagtgtgga ttttgcaagt ggtcacctga aaaacacgga
      301 tgaatctgat ggtggagcaa agctagtcag cctgcagtta cgcgatgagg ctcccggtgc
      361 aggtagtaaa attatccaag ccggtaatgg taatcaggct acaagtactc aatatgtaga
      421 tgtaatttcg ggtactgcaa acctgcctta ctcagttgaa tattttgcag atggtacggc
      481 caccgcgggt aatgtaacca gtaatgtagt ctatttcatc cagtacaaat aatcacacaa
      541 agataatata tcaataacgt atttaaggag ggccccccct ccttattaag aggtctcaaa
      601 atgtttacca gatgttattt aaaccacaac ctgatgaaat ctttacctct gttatttgtg
      661 actttattca gcctacattc agaagccagt atagttatta cgggaacccg ggtaatctat
      721 cccgccgggg aacattccgt cagcgtgaaa ctgaataaca atggcaagct tccggtgttg
      781 attcaaagtt gggtggataa tggtgaccca gataaaaaac cagatgattc tcaggcccca
      841 tttattatta cccccccgat taacagagtt gatcccggaa aaggacagac cttaagagtt
      901 accttcactg accttccatt acccaccgat cgtgagtctg tcttctggtt aaacgtgctg
      961 gaaatcccaa ccagatatga accaaaatca aatgataatt atcttcaggt agcatttcgt
     1021 accaggataa agctttttta ccgaccagaa aatttaccag gaaaatctct ggaggcaccg
     1081 aatacactca tctggaagat ttctagtggg caactgatgg catcaaataa ttcggcatgg
     1141 aacgtatctc tttccagtat ttcagtttgc ggaaagaaag tcgagggaaa aatggtcagc
     1201 ccaagaggat ctgaaatttt cccactaaaa gtatccccgg gtatgcgggt aagctttgaa
     1261 gctgtcaatg actacggagc aatcatgaag aaaacagcaa ccgtacagta ataaatatac
     1321 ataaaggtta ataccgtgga aaacagaata tttaaaccac acattattgc cgtaacgata
     1381 gcaggactgt atggcttttc tgttcatgct gtctttgcga atgatactat aaactcgtcg
     1441 gacagcccgg tcgctcaaga aaatacgaag aaatccctac catccccaac gtctgagcgt
     1501 attgaatttg ccactgaggt actcgaacta aaaaacaaac aatcaattga tttaagtcgt
     1561 ttttcttccg gaaactattc tctaccagga aaatatcagg caggcatatg gattaatgat
     1621 agttttcttg gaaaccaaga cattgaagtc aaagggcata cagatggttc ttcttatgtc
     1681 tgtataacgc ctgatctgct gaagatactt ccattatctt gggacagatt aagtgagtat
     1741 aaattcaatc aggcccccga atctaccaac tgttctgatg ttttagccag tataccggat
     1801 tcccgtgtaa cgtttgatag cggagaacaa cgtcttgatg ttgctattcc tcagaaattc
     1861 attatccatc aggctcgcgg aagtgtcagt cctgatttat gggacagtgg aatacccgca
     1921 gcttttctca gctacaacat gaacagctgg gcaagtcagt cacgtgggta tgattataaa
     1981 tctctttata cctcggttaa ctccggtatg aatattggtg catggtattt ccgtcataat
     2041 ggttcttaca catggtctga atctggcgag aaagattatc agtcgattaa tacctatgca
     2101 cagcgtgata ttctttccat taagggacga ttactcattg gccagtcaaa tactaccggt
     2161 cgactgtttg ataccgtccc cttttccggg gtgcaactgg caagtgatga gagaatgtat
     2221 ccctcatcgc aacgtggtta tgccccggag atacatggta tagctaagac tcacgctaaa
     2281 gtgactgtcc gccagaatag tgtggtcatt tatgagacaa cagtttcacc gggtgaattc
     2341 gttattgatg atttataccc gacgggattt ggtggagatc tcatggtcaa tattcatgaa
     2401 gcagatgggc aggaaagata tttcacagtg ccttttgcct caacaccgca attgctgagg
     2461 cctggatcat atcgttactc attcaccggg ggaaaattac gccaaatttc actgtcagaa
     2521 aaaccacagt tttatgaagc aacatggcaa cacgggttga caaactttct ttcaggttat
     2581 ctggggtggc agcagagtga aaactattat gcagctcagg ccggtttagc tgttggttca
     2641 ctggcaggct ccctgtcttt cgatatcact cagtcccacg ctacttttga taaatccgga
     2701 cgaaataccg gtccattatc aggtaatgac agctcttcat cgggtcaaag ttaccggctc
     2761 agctattcaa aattaattca gaatacagga acgtccataa acctagctgc ctatcgtttt
     2821 tcaacagaag ggtattatga cttcactact gcgatgatga cccgtgaggc gattaaacgt
     2881 ggttacgatt cgaacgtcat taaaagagcg aaaagtcgtt acactctgag cgtgaaccag
     2941 tcattggcgg aaagtctggg acagctttac gctagtgctt cactgcaaaa atactggaac
     3001 agtgacgatt cagataaaca gtatcagctt ggttacagca atcacaggaa attagtttca
     3061 tacggaataa gtgtcagccg aagctattcc tggtatggaa aaagtgatac cagttggatg
     3121 gcatccgtga gctttcccct tgggaatgtc agtgaggagc atacaccaaa tattcgcttt
     3181 gatgccgtac gtacgtcagg tggccattac tcagagtcgg cctcaatctc tggaaatgcg
     3241 ggggtcgaaa accagtatag ctatggagtg acagccagca atgccggcgg agataccggg
     3301 aacagtctgt ttgttaacgg gcaatggcgt acgccttata catacctgag cggtagcaat
     3361 agtaatggca ggaattaccg gagccagtct cttggtgcca gtggttcact gattgcgcat
     3421 tccggtggag tgacactcag cccgtacata tcagatacct gggcactggt tgaagccaag
     3481 ggcgcaaagg gagccagagt atcatcatat ccgggggtta tggtcgatcg ctttggatat
     3541 gcagcagttc catatcttaa tgcctacgaa cttaatgaag tggctataga tccaaacaat
     3601 gccgataatg acgttggttt tgagaacaca tcccgtaagg tagctcccta ttctggcgca
     3661 attgtgaagg tcaaatatga tacacagacc ggggttccag tattgattga tgcaggatgg
     3721 aaaggacaac ctataccatt cggtgccgat gttattgatg agagcggtaa ttatatggcg
     3781 actgcaggtc aggcaggaca aatttttaca aggcttgaac ccggcaggca tgtgctgaag
     3841 ttaaaagcag gaagccaagg cagttgcagt ctccccgttg aaatccccga gaccgtatcg
     3901 ccgtcaaacc tgctcagatt
//
LOCUS       DAZFFG010000236         3840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFFG010000236 DAZFFG010000000
VERSION     DAZFFG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.237"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            56..1441
                     /gene="dcuC"
                     /locus_tag="ACHJ7J_004920"
     CDS             56..1441
                     /gene="dcuC"
                     /locus_tag="ACHJ7J_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5003011.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1481..2161)
                     /gene="dpiA"
                     /locus_tag="ACHJ7J_004921"
                     /gene_synonym="citB"
     CDS             complement(1481..2161)
                     /gene="dpiA"
                     /locus_tag="ACHJ7J_004921"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO5003012.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2130..3788)
                     /gene="dpiB"
                     /locus_tag="ACHJ7J_004922"
     CDS             complement(2130..3788)
                     /gene="dpiB"
                     /locus_tag="ACHJ7J_004922"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO5003013.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          881 a          993 c          931 g         1035 t
ORIGIN      
        1 ttaagtttta ggaatttttt attatttact ttggggcctg gagacaggaa aaattatgct
       61 gacattcatt gaactcctta ttggggttgt ggttattgtg ggtgtagctc gctacatcat
      121 taaagggtat tccgccactg gcgtgttatt tgtcggtggc ctgttattgc tgattattag
      181 tgccattatg gggcacaaag tgttaccgtc cagccaggct tcaacaggct acagcgccac
      241 ggatatcgtt gaatacgtta aaatattgct aatgagccgc ggcggcgacc tcggcatgat
      301 gattatgatg ctgtgtggct ttgccgctta catgacccat atcggcgcga atgatatggt
      361 ggtcaagctg gcgtcaaaac cattacagta tattaactcc ccttacctgc tgatgattgc
      421 cgcctatttt gtcgcctgtc tgatgtctct ggccgtctct tccgcaaccg gtctgggtgt
      481 tttgctgatg gcaaccctgt ttccggtgat ggtaaacgtt ggtatcagtc gtggagcagc
      541 tgctgccatt tgtgcctccc cggcggcgat tattctcgca ccgacttcag gggatgtggt
      601 gctggcggcg caggcttccg aaatgtcgct gattgacttc gccttcaaaa cgacactgcc
      661 tatctcaatt gctgcaatta tcggcatggc gatcgcccac ttcttctggc aacgttatct
      721 ggataaaaaa gagcacatct ctcatgaaat gttagatgtc agtgaaatca ccaccactgc
      781 tcctgcgttt tatgccattt tgccgttcac gccgatcatc ggtgtactga tttttgacgg
      841 taaatggggt ccgcaattac acatcatcac tattctggtg atttgtatgc tgattgcctc
      901 cattctggag ttcctccgca gctttaatac ccagaaagtt ttctctggtc tggaagtggc
      961 ttatcgcggg atggcagatg catttgcaaa cgtggtgatg ctgctggttg ccgctggggt
     1021 attcgctcag gggcttagca ccatcggctt tattcaaagt ctgatttcta tcgctacatc
     1081 gtttggttcg gcgagtatca tcctgatgct ggtattggtg attctgacaa tgctggcggc
     1141 agtcacgacc ggttcaggca atgcgccgtt ttatgcgttt gttgagatga tcccgaaact
     1201 ggcgcactct tccggcatta acccggcgta tttgactatc ccgatgctgc aggcgtcaaa
     1261 cctcggtcgt accctgtcac ccgtttctgg cgtagtcgtt gcggttgccg ggatggcgaa
     1321 aatctcacca tttgaagtcg taaaacgcac ctcggtgccg gtgcttgttg ggctggtgat
     1381 tgttatcgtt gctacagagc tgatggtgcc aggaacggca gcagcggtca caggcaagta
     1441 aatagtaatg ccggaggcag tttcctccgg ctttatcatg tcacccactg tggtatatgc
     1501 gttgcggtct gccaactttg ccgtgaacaa tttcagcaat aatcagatgg cggctggcgc
     1561 aatattcaag ataacgcctg gcagtggtgc ggctgatggt cagtgcctgc gctaccgttt
     1621 ctgccgtatg ttgcacacca ggctctttga acaattttcg caccgcgttt agcgtcaagg
     1681 ggtcaatgcc ggtcggcagt tcgtctttgg gttcaccgcg agcataggca ttaaacatct
     1741 catcaatttg cttctggctg gcgctatcaa tactttccag catatgttta cgctggcgga
     1801 aacgggttag cgtttgcccc agccgttcat aggcaatggg cttaatgaga taatcaaata
     1861 caccacaacg tacggcttca gacaccgttt ccatatcgct ggctgcagtg gtaaacacca
     1921 cgtcgccggg ataatgcgcc tgcaccagtt catgcagtaa attaatccct ctaccgtcag
     1981 gaagatagtt atcgagcaag attagccccg gcttaaaacg ctcgatcatc attcgggcct
     2041 gcgccaggtt tcccgccagt aatatctgac tgaatccggg aatgtgacga atatattccg
     2101 catgcatctc tgccagcggc gtttcgtcct caacgatcaa tagggttaat ggagctgtca
     2161 ttaggtttca ctttcggaat atagattgaa aataaggtac cgcagggatc attatcttcg
     2221 agagtgataa caccaccgca gcgcgttaca tagctggcaa tcaagtacaa cccaatgcca
     2281 tgttcaccgg gctcgtcagc acgcgtactg accccctgct caaatatttt gtctcgtaga
     2341 gactctggaa cgccgcagcc ctgatcggcg acttcaatca ccacatcatc gccttcatcg
     2401 ctgaggaata attcaacgat cttgtttcct tcatcgctac gcaggctggc ttcgaaggcg
     2461 ttatcaagta aattgccaac aatggcggca aactcggtgc tatccagtcc tggcggcagt
     2521 tgcgaaagct ggctaccggg gacgatgacc atttttagcc ccagttcgcg ggcgcgctgc
     2581 actttaccaa aaagcagccc cgccacctgg cgatcggcaa acgcctcacg caggctgtca
     2641 ataagctgtt gctgggcctg agactccccc tgcaccatcg ccagcacgcg atcatactct
     2701 ttcatctgca acagaccatt gagcgtcgac atccaattga gatgctcgtg tcgcaatgta
     2761 cgaaggctct cgacgtattg ttttatttgc gtcagttgcg cattgagggt ggaaatttcg
     2821 tctttactac gaaagctgat aatggccccc agcaaatcat cacctgaacg aatagcttcc
     2881 cgattggcaa taacgctcag accgttaaag ttagccacca catcctgacg tttttcatca
     2941 atctgttcgg taaagaaatc ggcgggcctg accacttcag caatgggttt acccaaccat
     3001 tgccgtccgg gggaactcag ccccagcatc tttcttgcgt tacgattgat ggcggtaatg
     3061 taaccgtgcg gatccaccgc aatcagccct tcataaaccg aactaaacag cgcctcttgc
     3121 tgacggacca cgcgtgcgat ttgctttggc tccatgccca tcatctgccg acggatatgc
     3181 gcggccagga accacgacag caacatcaga atccctaaca gcacgacaaa cacacctgcc
     3241 atcggtaata aaaactcagc ccgccagcta tcgattttac tcaccaggta gccaatcgac
     3301 accacgccga tgacttttcc atcgtcatca aagattggcg ttttggcgcg catcgccata
     3361 cccattgacc ctttcccggt aatgaagtag ctctcccctt tctccagcgc gccctgtttg
     3421 gtgaactgca taggataacc aattttctcc ggattaggat ggtaaaggcg gatcgagtgc
     3481 cggtcgccaa tcaccacata atcaaaatcg gtatctcttt gtaatttgtt agcgatggtc
     3541 gccagccgtt tgtagtcacg cgtttttact gcgctgatga tactgtcatt ggaggcaata
     3601 attttcgcct gattcattgc catgtcgcgt acatgaagcg ttaaatagtc ctcaaaactg
     3661 gccgtaaaat attgcgccag ggctgcaatg acaaatattg agaacaccag aatcagcaaa
     3721 aagatacgca gcggaaatgc cagtctttgg aaaaatgcaa actgtttatt ctcgttaagc
     3781 tgcaacattg ttttccttgc gggttgtccc gagcctcttt gacggatgta attaaaatgt
//
LOCUS       DAZFFG010000237         3824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFFG010000237 DAZFFG010000000
VERSION     DAZFFG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.238"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1082
                     /locus_tag="ACHJ7J_004923"
     CDS             <1..1082
                     /locus_tag="ACHJ7J_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003014.1"
                     /translation="GPMQLEVPRDQYAGAVETMKNKIREGKVPGVTDPAEASRLIRRG
                     HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRDAALQT
                     AAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRQALQKGTGAGNIS
                     ALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSGVAGGAVGSVA
                     GGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWL
                     AGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPV
                     MEYDASHLGKMVNRLEEALPPELPA"
     gene            1158..1613
                     /locus_tag="ACHJ7J_004924"
     CDS             1158..1613
                     /locus_tag="ACHJ7J_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO5003015.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            1714..1923
                     /locus_tag="ACHJ7J_004925"
     CDS             1714..1923
                     /locus_tag="ACHJ7J_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5003016.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGTHFRLVIRNAEGQLRW
                     RCWNFESDAGKQLNPYLASEGILRQ"
     gene            2013..2831
                     /locus_tag="ACHJ7J_004926"
     CDS             2013..2831
                     /locus_tag="ACHJ7J_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5003017.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSVVLPPPARQALAQAALTYRYGDEHQPITTADILTPQRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            2923..3408
                     /locus_tag="ACHJ7J_004927"
     CDS             2923..3408
                     /locus_tag="ACHJ7J_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5003018.1"
                     /translation="MTTFSHSSITPSVSVTTASGNNQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYNHHACRTECYAMTVHYYRLRDYALQHPECRAIMRII
                     D"
     gene            3424..>3824
                     /locus_tag="ACHJ7J_004928"
     CDS             3424..>3824
                     /locus_tag="ACHJ7J_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5003019.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALCHNAAA
                     VILAHNHPSGEVTPSKADRLITERLVQALALV"
BASE COUNT          892 a         1026 c         1027 g          879 t
ORIGIN      
        1 atggtcccat gcaactggaa gtcccccgtg atcagtatgc cggcgctgtg gaaaccatga
       61 agaataagat ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc
      121 tgattcgtcg gggacatctg acttataccc aggcccgtaa tatcacccgg ttcgggacca
      181 tcgaatcggt cacttatgat attgccgagg gttcggttgt cagtctggcg gccggaggga
      241 tcagttttgc cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc
      301 tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca
      361 caacccagca acttcaccgg ctcagtgttg ttcagggtat gctgaagcat attgattttt
      421 cgacggccag cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca ggaaatatca
      481 gtgccctgaa caaagtgatg aagggtacgc tggtgacatc tctggcactg gtagctgtca
      541 caaccggccc tgacatgatc aaaatgttgc ggggacggat ctccggtgcg cagttcatca
      601 ggaatcttgc cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg
      661 ggatattgtt cagtccactg ggaccatttg gtgcactgac aggacgtgtg gttggcggtg
      721 ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag
      781 aagatcgcgt caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt
      841 tcctgctgac cggacatgag attgaaaatc tgaacgcgaa tctggcccgt gttatcgatc
      901 agaatgctct ggagatcatt ttcgccgccg gtatacaaca acgggccgcg accaatatgt
      961 taatcaaacc actggtggtc agtatcatca ggcaacgccc cgtcatggaa tatgatgcat
     1021 cccatctcgg caagatggtt aaccgactgg aagaagcatt gcccccggag ttaccggcat
     1081 aacggctatt ctgcgccttt tacgttttat cacctgattt gtcagtgtca tcgtcatgac
     1141 tcagagaggt aaataccatg attcacctgt tcaaaacctg catgattacc gccttcattc
     1201 tggggttaac gtggtctgcc ccactccggg cacaggatca acgttacatc agtatacgca
     1261 atacagatac gatatggctc ccgggaaata tttgtgccta ccagttccgg ctggataatg
     1321 gcggaaacga tgaaggattt ggccccctca ccatcactct gcaactcaaa gacaaatatg
     1381 gtcagacgct ggtgaccaga aaaatggaaa cggaagcctt tggtgacagt aatgccacgc
     1441 gaaccacaga cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca
     1501 ttaaagcaac tgaagaaagt aacggccatc gtgtcagtct gccgttatcg gttttcgatc
     1561 cccaggacta ccatccactg ctgattaccg tttccggaaa aaatgttaac tgatccctga
     1621 agtcagtttc atgcttcagc ctctgccata cgttgtggca gaggctattt atcaggagaa
     1681 accagatatg ccgggttaca cacagagtat gtcctggctg aaggatcttt ctcatacggg
     1741 caggcggttg ctgtcataac cgcttaccgc aatgtgttta tccaggatga cccgggtacg
     1801 cattttcgtc tggtgatccg caacgctgaa ggtcagttgc gctggcggtg ctggaatttt
     1861 gaatctgatg ccggaaaaca gctaaatccg tatctcgcca gtgagggaat tctcaggcaa
     1921 taaacgtctt catttcatcc atcaggccgc gtcttctccg ggagacgcgg ccttttcgtt
     1981 tataccgcta actcattcat aaggagcaaa gtatgcaatt agccagtcgt tttggtcatg
     2041 taaatcagat ccgtcgggag cgcccactga cacgcgaaga actgatgtac cacgtcccga
     2101 gtatttttgg agaagaccgg cacacctccc gcagtgaacg gtatgcgtac attcccacca
     2161 tcaccgtcct ggaaaatctg cagcgggaag gctttcagcc gttcttcgcc tgccagaccc
     2221 gtgtgcgcga ccagagccgc cgggaatata ccaaacatat gctgcgtctg cggcgggccg
     2281 gacagataac cggtcagcat gtgcctgaaa ttattctgct caactcccat gacggttcat
     2341 ccagctacca gatgttaccc ggatattttc gtgccatctg caccaatggc ctggtctgcg
     2401 gtcagtcgct gggagaagtc cgggtgccac accggggaaa cgtggtggat agggtgattg
     2461 aaggtgctta cgaggtggtg ggggtttttg accggattga agagaaacgt gatgccatgc
     2521 agtcggtggt cctgccgcca ccggcacgcc aggcactggc gcaggctgct ctgacttacc
     2581 gttatggtga tgaacatcag ccaatcacca ctgctgacat tctgacgcca caacgccggg
     2641 aggattacgg taaggacctg tggagtgctt atcagaccat ccaggagaat atgctgaaag
     2701 gcgggatttc cggtcgcagt gcaaaaggaa aacgtatcca cacccgtgcc attcacagca
     2761 tcgacaccga tattaagctc aaccgcgcat tgtgggtgat ggcagaaacg ctgctggaga
     2821 gcatgcgctg atgctgtttc cctgaaagtg cagccctgac tcacgtctgt accagtcccc
     2881 aagcattctt ccattcattt actttttata aggaatcatc tcatgacaac cttttcgcat
     2941 agctccatca caccttctgt ttccgtaacc actgcatcag ggaataacca gtctcaactg
     3001 gttgccactc ctgtccctga tgagcagcgc atcagcttct ggccgcaaca ttttggcctc
     3061 attccacagt gggtgaccct ggagccccgt gtcttcggct ggatggaccg tctgtgtgaa
     3121 gactactgcg gtggtatctg gaatctgtac accctgaaca acggtggcgc atttatggca
     3181 cctgaaccgg atgaagatga tggagaaaca tgggtactgt tcaatgccat gaacggtaac
     3241 cgcgctgaaa tgagcccgga agccgccggc attgccgcct gtctgatgac gtacaatcat
     3301 cacgcctgtc gtacggagtg ttatgccatg acggtccatt attaccggct gcgggattac
     3361 gccctgcagc atccggaatg ccgcgccatt atgcgcatca tcgactgaat ggaggcaggg
     3421 acaatgcagc agctttcctt tctgcccgga gagatgacgc ccggcgagcg cagtctcatt
     3481 ctgcgggccc tgaaaaccct ggaccgccat cttcatgaac ccggcgtggc cttcacctcc
     3541 acccgagcgg cacgggaatg gctgattctg aacatggctg gactggagcg tgaagaattc
     3601 cgggtgctgt atctgaacaa ccagaatcag ctgattgccg gtgaaaccct cttcaccggt
     3661 accatcaacc gcacggaagt ccatcctcgg gaggtcatta aacgtgccct gtgccacaat
     3721 gccgctgccg tgatactggc gcacaatcac ccgtccggtg aagtcacacc cagtaaggct
     3781 gaccggctta ttactgaacg tctggttcag gcactggccc tggt
//
LOCUS       DAZFFG010000238         3650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFFG010000238 DAZFFG010000000
VERSION     DAZFFG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.239"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..490
                     /locus_tag="ACHJ7J_004929"
     CDS             <1..490
                     /locus_tag="ACHJ7J_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFO5003020.1"
                     /translation="ITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIITLV
                     EHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEMIL
                     DNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNILK
                     TI"
     gene            522..1814
                     /gene="sctN"
                     /locus_tag="ACHJ7J_004930"
     CDS             522..1814
                     /gene="sctN"
                     /locus_tag="ACHJ7J_004930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFO5003021.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKQSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1804..2262
                     /locus_tag="ACHJ7J_004931"
     CDS             1804..2262
                     /locus_tag="ACHJ7J_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO5003022.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYERLKTRIKKQLLDQMLYQDELEQEEKYNGRSQEN"
     gene            2240..2470
                     /locus_tag="ACHJ7J_004932"
                     /pseudo
     CDS             2240..2470
                     /locus_tag="ACHJ7J_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            2768..3115
                     /locus_tag="ACHJ7J_004933"
     CDS             2768..3115
                     /locus_tag="ACHJ7J_004933"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFO5003023.1"
                     /translation="MFTQHIEKQRNVHTLNQNDINNSVNSVNVPENELTYQFQRWGQN
                     HTVRILESSEGIRLKPSDALVSDRLHEAQNNDVTAQRWVLTEQDERQGQRHQPHEEQE
                     NEGKFENDQKDES"
     gene            3075..3650
                     /locus_tag="ACHJ7J_004934"
                     /pseudo
     CDS             3075..3650
                     /locus_tag="ACHJ7J_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT         1224 a          647 c          825 g          954 t
ORIGIN      
        1 tataaccgtg cagcgcattt cagaggtaat tcagcgtctg atccaggagc gaatttcagt
       61 gcgcaatatg cggttagtta tggaggcctt agcattatgg agcccacgag agaaagatat
      121 aatcacactc gtcgagcatg tacgaggtgc attaggtcgc tacatttgcc ataagttttc
      181 atattctgga gaaattaaag caattgtgat ttcgcctgaa atagaggaca gaatcagaga
      241 tggtgtcagg cctaccgctg gcggtacttt tcttaatctc gatgcatccg aagctgagat
      301 gattctggat aattttaaac tggcactttc tggaatcaat attcctatta aagacattat
      361 tttactggga tctgtggata ttcgtcgttt tatcaaaaaa cttattgaat ctagttatcg
      421 agacctcgaa gtactttcgt acggggaact aacggaaaat gttcccgtta atattctgaa
      481 aactatttag gataatgtct tgcgatatgg agcatgctgg tatgaaaaaa ttaaaattat
      541 taaataaata cagctactta catagcataa acggctctct gattgaagca gaacttgacg
      601 acgtttctgt gggtgaagta tgcgaaattt atgcgagctg gcaagcaaat gaacgtatag
      661 ccagggctca ggtcgttggc tttcgcaatg gaaagacatt attaaacctt ataggcagct
      721 cagtggggct tacacgtact gcagttttga aaccaacggg agagcagtta accatacaga
      781 tcagtgatgc ctttcttggt tcggttttga atgcctctgg tcaaattatg gaaaggtttg
      841 tcccggacac tccaggcgat agaggaaatt tacgcctgat tgatgaactg ccaccgtcgt
      901 atcaggaacg tcgagttatt aatacaccac tagagacacg cataagagtc attgatggcg
      961 tactgacatg tggcattggt caacgtgttg ggattttcgc atcggctggc tgtggcaaaa
     1021 cggttttaat gcatatgctc gtaaacaata ctgaagctga tgtgtttgtt attggcttga
     1081 ttggcgaacg tggtagggaa gttacggaat gcgcggaatc gctgaaacaa tcagtaaatg
     1141 cggcaaaatg cgtattggtt tatgccacct ctgatttttc gtcagtagat cggtgtaatg
     1201 ccgccctgat ggcgacaacc gtggcagagt attttagaga tcgaggaaag agagttgtac
     1261 tttttattga ctcaatgacg cggtatgcac gagcactacg tgatatgaaa ctggcagcgg
     1321 gtgaaccacc tgccagacgt ggttatcctg cttctgtttt tgacagtttg cctcgcttac
     1381 tggagaggcc gggaccaaca ctaaaaggaa gtatcaccgc gttttatacc gtgttactgg
     1441 aaggggaaga tgaatcagac ccactgggtg atgaaattcg ctcaatcctt gatggccata
     1501 tctatctaag ccgcaaactc gcgggtcaag gtcactatcc ggcaatagat gttctaaaaa
     1561 gtgttagtcg agtatttggt caggtcactg atgaaaaaca cagagataat gctgcacggg
     1621 taaggaagat cctcacaact ttggaagatc tacaggtttt tattgactta ggggaatatc
     1681 gcgctgggca aaatgctgaa aatgattttg ctatgaatgc acggcctaaa ctcacaaatt
     1741 ggttgaaaca gtcagttaat gaaaaaatgc caatgagtga aactctaaaa gagcttgaac
     1801 gaattgttaa gtaaagttaa tcgacttata agaagaacag cgcagagcct ggctgcatgt
     1861 gaagcatcat tacaaaagtt aaatgcagaa aaagaaaagt tagccgaaaa ggaacgcctg
     1921 tatgatatgc aacttaaaaa cctgcagtca ctgttagata tgaaagagtt actcggcgaa
     1981 gttgtatttc gccaggatat attttactcg ttacgtaaag ttgcagtcat tcagcagcag
     2041 atcgctgaga taaatcttga aaagcaaaaa atagcagagc ggagaaaaat acttaacaag
     2101 gaaatagttc aacagcaagc acaaagaaaa cactggtggc taaaaggaga aaaatacgaa
     2161 cgactaaaaa cgcgcatcaa aaaacaactt ttagatcaga tgctttatca agacgagctt
     2221 gaacaggagg aaaaatataa tggacgaagt caagaaaatt gaacacggta aaattttaaa
     2281 cacagacaca cccgataatt ctgcttcgga tttagacagt ttcttaaaga aaaacaaaaa
     2341 aaataaagaa atgagtgaga ttgtcgctgg aaccattctc cagttattac gtcagactca
     2401 ggatagccaa ttttctacgt tggggaaaaa aaatgggcaa atgaagtcta ttgacaacaa
     2461 atcatctctc caggttgaca ggaatccctc tctttctttg caaacaaatg gaacagagca
     2521 attttctaca aagaaaattc tcaatatgac ttctcgaggt cagggtatta gttctgaact
     2581 atctgataac acgataaaaa ttaaggaaaa aatccataat ggtagccata cagagtacat
     2641 cacagatcaa aaaaacagta ataataaaga tcgtgaaaaa aaataccgcg atggagacaa
     2701 aataaatgga ccgcaggctc attcattaga cagtactaac gaaagaaggt ttgctgataa
     2761 cagaaccatg tttactcaac atatcgaaaa gcaaaggaat gttcatacgc tcaatcagaa
     2821 tgatataaat aattccgtta atagtgtcaa tgtgccagaa aatgaattga catatcagtt
     2881 tcagcgttgg ggacagaatc atacagtccg gattctggaa agtagtgaag ggatacgact
     2941 aaaaccatca gacgcgcttg tctccgacag actccatgaa gcccaaaaca atgatgttac
     3001 agcacaacgt tgggtattga ctgagcaaga tgaacgacaa ggacaacgac atcagcctca
     3061 tgaggagcaa gaaaatgaag gcaaattcga aaacgatcag aaggatgaaa gttaatgttc
     3121 ggtctgagga aagtaaatcg aaacactcat acgtttgaaa ccacttttca aaattggaaa
     3181 gaaaatggcg aagatgttgc attattaatg cccgaattta gtgctaaatg gctaccaata
     3241 gcagaagaga gtggcagctg gtctggatgg gtattgcttc gagaaatatt tcccttaatt
     3301 tcttctgagc tcgctgggat ggctttaatg ccagaaacgg aaaggttaat aggggaatgg
     3361 ctcagtttat ccagttcccc gttaaatcta aagcatcctg aactaaaata taatcgtttg
     3421 tgcgtaggta aagtatttga tggagtattg agcccagctc aaccactaat aagaatatgg
     3481 acaggggaat tgaatctttg gttagataaa gtcacagtct gccaatacgg aaacgctcca
     3541 gcgttagaca aaaaatcatt atattggccc attcattttg taattggatt tagtaaaaca
     3601 tgttacagaa gtcttgttga tattgaagtt ggtgatgttt tattaatctc
//
LOCUS       DAZFFG010000239         3554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFFG010000239 DAZFFG010000000
VERSION     DAZFFG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.240"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..263
                     /locus_tag="ACHJ7J_004935"
     CDS             <1..263
                     /locus_tag="ACHJ7J_004935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003024.1"
                     /translation="LLKKDKMNLCVPSFPSEHIHYSNFTQPRTNKKHHPGYCRYRFPA
                     GKNHKQNTGNNAFRNPPLLKKNKIKKTTVPALNAEYNILWTG"
     gene            1101..1973
                     /locus_tag="ACHJ7J_004936"
     CDS             1101..1973
                     /locus_tag="ACHJ7J_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5003025.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            2345..>3554
                     /locus_tag="ACHJ7J_004937"
     CDS             2345..>3554
                     /locus_tag="ACHJ7J_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5003026.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRTGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVLGTANGMAISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDG"
BASE COUNT          965 a          915 c          891 g          783 t
ORIGIN      
        1 tgctgctgaa gaaagacaaa atgaatctgt gtgtcccctc cttcccttca gagcacatcc
       61 actattcaaa ttttacacaa ccacgtacga acaaaaagca tcatcccggg tattgcagat
      121 accggtttcc tgccgggaag aatcataaac agaacaccgg gaataacgcc ttcaggaatc
      181 cccccctcct gaagaagaat aaaataaaga agactacagt tcctgcactg aatgctgagt
      241 acaatattct atggacagga tgacatattt ataaataaca aaaaccattt aatactgata
      301 catcaataaa atacttcctc cctgctgacg cagataaaat gcaccatttt ttcttcaaat
      361 acttctgtat aatcgccagc caatgatgag aatggattaa aataacactt cttcgacaaa
      421 cacaaaaaca gccttcattt tacatcatca tccagtttca gttaaccccg gacgagcgac
      481 agcgtttcag tacaggaatt aaaagccagc ttttgctcag taaaaacaca ctcaactaat
      541 aatatctcag cagatattat cctgaccagg acacctcaca ttactgaatt ctcttccgac
      601 agtatcactg cacaaataaa aaagaacggg ataaatattc agaccggaag aatggtgtca
      661 caaaagcatt taatcattac gcatgaccag gaagggaaac acttaaccac atggtatcaa
      721 cagatatcat tacgtgtgca ctcttataaa aaacacacag aagaagtaag ataattataa
      781 acgtaaattt aatatctccc actgacatct cccccacaat acactgaaaa atactcacag
      841 cagtaaacac agacacacct gataatcatg cagccgataa atattttccc ctttacggag
      901 aaatcagcct gctgtttaac aataacggcg tattttcatg gaaggagatc acccagtaac
      961 ggataagtcc tgatctcctt catgttaata cgcaacaatc ccatcgattc aaaacaattt
     1021 cagacagata ttatctccgt gaatcccccg ccacctttcc ggtgcgcggg gttttgtctt
     1081 ttttcaccgg gaatacatgt atgaatccgt ctgatgccat tgaggcaatt gaaaaaccgc
     1141 tctcctccct gccttactcg ctttcccgtc acatcctgga acatctgcgc aaactcaccc
     1201 gtcacgaacc cgtgattggc attatgggta aaagcggggc cggtaaatcc tcactctgta
     1261 atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc acccgggaag
     1321 tacggcgctt ccgtctgagt ggccacggtc acagcatggt tatcactgac ctgcccgggg
     1381 tgggcgagag ccgggacagg gatgcagagt atgaggccct gtaccgtgac attctgcctg
     1441 aactggacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct gtggatgagt
     1501 atttctggcg acacatcctg caccggggac atcagcgggt actgtttgtg gtgacgcagg
     1561 ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct tctcctgcac
     1621 aggcacagaa tattcgcgaa aaaacggagg cggtattccg tctgttccgg cccgtacatc
     1681 cggttgtggc cgtatcggcc cgcaccggct gggaactgga tacgctggtc agtgcgctca
     1741 tgacagcgct tcccgaccat gccgccagtc ccctgatgac ccgactgcag gacgagctgc
     1801 gcacggaatc tgtccggtct caggcccgtg aacagtttac cggtgcggtg gaccggatat
     1861 ttgacacggc ggagagcgtc tgtattgcct ctgttgcacg cacggttctg cgtgccgtcc
     1921 gtgactcggt ggtctctgtt gcccgcgctg tatggaactg gattttcttc tgaacactct
     1981 gccaggggaa atgcctgatt atattgcatt aaaaaactga aaacatgaca caaacccctc
     2041 ataccctcct gtttctcttg cgtgaccgcc ccgccatcaa tagaataaaa cgatcgataa
     2101 aacaggtatc gatcgttact atcgatcgtt tatagtggtt aatggcctgc gaaaaggcag
     2161 ctgaatctct tcatcatgca gaacggaatt gcacacaacg gactgatact tctctggcgg
     2221 atgagagagg ggaagcattt atgtccggga aaaaccactt tatctgacca tgctgttttg
     2281 tacctgccgg tatccacatt tgtgggtacc ggctttttta ttcaccctct gtaaggaaaa
     2341 gctgatgaaa cgacatctga acaccagcta caggctggta tggaatcaca ttacgggcac
     2401 cctggtggtg gcctccgaac tggcccgctc acggggaaaa cgcaccggtg tggcggttgc
     2461 gctgtctctt gctgctgtca cgtcagtccc ggcactggct gctgacacgg ttgtacaggc
     2521 tggagaaacc gtgaacggcg gaacactgac aaatcatgac aaccagattg tcctcggtac
     2581 ggccaacgga atggccatca gtaccgggct ggaactgggg ccggacagtg aagaaaacac
     2641 cggtgggcaa tggatacaga atggcgggat agccggaaat accactgtca ccacaaatgg
     2701 tcgtcaggtc gtgctggagg ggggaacagc cagtgatacg gttattcgtg acggcggggg
     2761 acagagcctg aacgggctgg cggtgaacac cacgctgaat aacagaggcg agcagtgggt
     2821 tcatgagggc ggggttgcca ccggtacaat tatcaaccgc gacggttacc agagcgttaa
     2881 aagtggcggg ctggcaacag gaaccatcat caacaccggc gcagaaggcg gcccggattc
     2941 tgacaactcg tacacgggtc agaaggtcca gggaacagca gaatccacca ccatcaacaa
     3001 aaatggacgg cagattatct tattttccgg gatagcccgt gacactctca tttacgcagg
     3061 tggtgaccag tcggtacacg gaagggccct gaataccaca ctgaatggcg gttaccagta
     3121 tgtgcacaag gacggacttg cgctgaacac ggtaattaac gaggggggct ggcaggttgt
     3181 taaggcaggt ggtgctgtcg gtaacaccac cataaatcag aacggtgaac tgagggtaca
     3241 tgccggcggg gaagccactg cagtcaccca gaacacgggc ggtgcactgg ttaccagtac
     3301 ggctgcaacc gttaccggca taaaccgcct tggagcattc tcggttgtgg agggtaaagc
     3361 tgataatgtc gtactggaaa atggcggacg cctggatgtg ctgaccggac acacagccac
     3421 taatacccgc gtggatgatg gcggaacgct ggatgtccgc aacggtggta ccgccaccac
     3481 cgtatccatg ggaaatggcg gtgtactgct ggccgattcc ggtgccgctg tcagtggtac
     3541 ccggagcgac ggaa
//
LOCUS       DAZFFG010000240         3534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFFG010000240 DAZFFG010000000
VERSION     DAZFFG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.241"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..687
                     /gene="tssI"
                     /locus_tag="ACHJ7J_004938"
     CDS             <1..687
                     /gene="tssI"
                     /locus_tag="ACHJ7J_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO5003027.1"
                     /translation="RGTTLGNQLEVIPADRTWRPRLQSKPKVDGPQSAIVTGPAGEEI
                     FCDEHGRVRVKFHWDRYHGMTEASSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNG
                     DPDQPIVTGRTYHEDNRSPGDLPGTKTQMTIRSKTYKGSGFNELRFEDATDKEQVYIH
                     AQKNMDTEVLNDRTTDVKHDHTETIGNDQKITVVKGQTVQVGTKKEGATTRVSRWRTT
                     GVSQYVMTRL"
     gene            714..1136
                     /locus_tag="ACHJ7J_004939"
     CDS             714..1136
                     /locus_tag="ACHJ7J_004939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cons domain protein"
                     /protein_id="HFO5003028.1"
                     /translation="MSHDDGLYVRNDRKVTVEGKQEHKTTGNHISLVEGKHSLVVKGD
                     LARKVSGALGIKVDGDIVLESSSRISLKVGGSFIVIQPGGVDIVGPKINLNGGGSPGT
                     PVPTLQPTVLNTLVTNDTDSNTDDDIGNDETRVKFMFS"
     gene            1284..3356
                     /locus_tag="ACHJ7J_004940"
     CDS             1284..3356
                     /locus_tag="ACHJ7J_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFO5003029.1"
                     /translation="MIISPPILHAHIDTEKDSDWVNRMMSVDPERNFPMNSGRSWHGG
                     VHLNGNLSETVRCIADGKVISIRQPEPAENTVPPLNYNGVTDKGYVLIKHETEIGSGE
                     EGKIVYYSLYMHLRSIDSNVQPGNTLYRKEPVGAAGMVDGQNAFHFQIFCDDENIQKI
                     TGRISPETDLNKDGRTDVVYGDSHFYLPAGTPIYGDMPEENSLANNSPVQRTSVPLYV
                     TMSFDKGNCTMVTRQQHEVLSDTYTEVGSPLINADGKDYEYNLYSYALQLYPKSPSAG
                     YEMLRFGRVVNTEYEELVPADAPLWRTINTPQGKGVVNLGARDIRVYSDGDFPHWTGW
                     KLVNDDADTNSQCNSPTILCTTRNDLSRMICHFPLEWDSATVESRFEWLKSPNDVLSK
                     PMTEPDLDLLIEHCKALCLVDNPLPARRVWHFDPRQFIAHFRKCGWLGENDIISVVRR
                     YQNAYPMTSELTRPLSKDTLIERITSQGQNSSGEVIRPAGLKNELSKSLRKYLITTPL
                     RIAHYFGQMARETGRFASFIENGDSSYFDMYEPGTEQGLKLGNNVVGDGARFKGRGTI
                     HLTGRENYRNYGKYRNPSDDDFFTTEPNNQLPVENAYFSCDAGGYFWASKQKYIMVSH
                     RLTPSGSLGVNYWADKGCSLSDAREVTRRINPAADGFDLVRWPAFEHSWYVLNDEITP
                     PLNYIRIL"
BASE COUNT         1064 a          657 c          967 g          846 t
ORIGIN      
        1 agaggaacca cgctgggaaa tcagttagag gtgataccgg cagaccggac gtggcgtcca
       61 cggctgcaaa gcaaaccgaa ggtggacggg ccacagagcg ccattgtcac cgggcctgcg
      121 ggagaggaaa tcttctgcga tgaacatggc cgggtacggg tgaagttcca ctgggaccgt
      181 tatcacggga tgacggaggc gagttcatgc tgggtgcgtg tatcgcaggc gtgggcgggg
      241 ccggggttcg gtaatctggc gataccgcgt gtgggtcagg aggtgatagt ggactttctg
      301 aacggcgacc cggaccagcc cattgtgacg ggacgcacat accatgaaga caaccgttcg
      361 ccgggcgacc tgccgggaac gaagacacaa atgaccatcc ggtcgaagac ctataaagga
      421 agcggattta acgagctgcg ctttgaggac gccacagata aggaacaggt ctatattcac
      481 gcgcagaaga acatggatac ggaagtgctg aatgaccgga cgacggacgt gaagcatgac
      541 cacacggaaa ccattggtaa cgaccagaag ataacggtgg tgaagggcca gacggttcag
      601 gtcggtacaa aaaaagaggg ggccacgacc agagtatcac ggtggcgaac gaccggcgta
      661 tcacagtacg taatgaccag actctgaagg tgacgaatga ccggacggtg agcgtgagtc
      721 acgatgacgg tctgtacgtc agaaacgacc gtaaagtgac ggtggaaggg aagcaggagc
      781 ataagacgac ggggaaccat atcagtctgg tggaggggaa acacagcctg gtggtgaagg
      841 gggacctggc gaggaaggtg agtggcgcgc tggggataaa ggtggacggg gatatcgtgc
      901 tggagagcag cagccggata agcctgaagg tgggtggctc gtttatcgtt attcagccgg
      961 gcggcgtgga tatagtgggg ccgaagataa atcttaatgg tggggggagt ccggggacac
     1021 cggtaccgac actgcaaccc actgtcctga acactttagt cactaatgat acggatagta
     1081 atactgatga tgatatcggt aatgatgaga ccagagtgaa gttcatgttc tcgtagtggg
     1141 ataatcattc agataaagat aacggatgac ggtagtaata atacaataat actgtgattt
     1201 tctgctttct aagcagagat gctctgtgaa tgaataattc atactcgtta catgttttat
     1261 aagcttaata aggactattg ataatgataa tcagccctcc aatattgcat gcgcatatag
     1321 acactgaaaa agactctgac tgggtaaaca gaatgatgtc tgttgatcca gaaagaaatt
     1381 tcccaatgaa tagcggtcgt tcttggcatg gtggagttca tttgaacggc aatctgtcag
     1441 agactgttag gtgtattgcc gatgggaagg ttatttcaat acgccagcca gaaccagcgg
     1501 aaaatacagt tcctccgtta aattataacg gagttacgga taaaggctat gttctgataa
     1561 aacatgaaac agaaatcggt agtggagaag agggtaaaat cgtctattac tcattatata
     1621 tgcacctgag aagcattgac agtaatgtgc agccgggaaa cacgctatac cggaaagaac
     1681 cggttggtgc agcagggatg gtggacggac agaacgcctt tcattttcag attttttgtg
     1741 atgatgaaaa cattcagaaa atcacaggaa gaatatcacc ggaaacagac ctgaataaag
     1801 atggacgaac tgatgtggtt tacggtgata gtcattttta tctccctgcc ggaacaccga
     1861 tttatgggga catgccggaa gaaaactcgc ttgcgaacaa cagtccggta caaagaacca
     1921 gcgtgccatt gtatgtcaca atgagttttg ataaaggcaa ttgtacgatg gtgacgcgtc
     1981 agcagcatga agtgctgagt gatacctata ctgaagtagg ttctcccttg attaatgctg
     2041 atggtaagga ttatgaatac aatctctatt cctacgcgct gcaactctat ccgaaaagcc
     2101 caagtgccgg ttatgagatg ctgcgttttg gtcgggttgt taatacagaa tatgaagaat
     2161 tagtcccggc ggatgcgccg ctgtggcgaa caatcaatac gccacagggt aaaggcgttg
     2221 ttaacttagg ggcgagagat atcagggttt acagcgacgg tgattttcca cactggacag
     2281 ggtggaaact ggtgaacgat gatgcggaca ccaacagcca gtgcaattca ccaacaatcc
     2341 tgtgtacaac ccgcaatgac ctaagtcgga tgatttgtca ttttccgcta gaatgggaca
     2401 gcgccaccgt ggaatcacgg tttgagtggc tgaaatcacc caatgacgta ttgagtaagc
     2461 cgatgacaga gcctgacctg gatttgctga tagaacactg taaggcgctt tgtttggtag
     2521 ataatccgtt acctgccagg cgtgtatggc attttgatcc caggcagttt attgcacatt
     2581 tcaggaaatg tgggtggtta ggagaaaatg atattataag tgtggtaaga cgttatcaaa
     2641 atgcttatcc catgacatca gaacttacaa gaccattaag taaagacact ctaattgagc
     2701 ggattactag ccaaggacaa aatagcagtg gtgaggtgat aagacctgca gggttgaaaa
     2761 atgaactgtc aaagtcacta aggaagtact taattacaac cccattgcgg attgcgcatt
     2821 atttcggtca gatggcgaga gaaacaggca gatttgcgtc ttttatagag aatggtgatt
     2881 cgagttattt tgatatgtat gagccaggaa cagaacaggg acttaagctt ggaaataatg
     2941 ttgttggtga tggtgcaaga tttaaaggca gaggtaccat acatctaact ggtcgtgaga
     3001 attatagaaa ttatggaaag tatagaaatc cgtcagatga tgattttttt actacagaac
     3061 ccaataacca actcccagtt gaaaatgcct atttttcttg cgatgccggg ggatactttt
     3121 gggcaagtaa gcaaaaatac ataatggtaa gccatcgatt gacacctagt ggtagtttag
     3181 gcgttaatta ttgggcggat aaaggatgta gtttgtcaga tgccagagaa gttacacggc
     3241 gaataaaccc agcagcagat ggcttcgatt tagtgcgttg gccagcattt gagcattctt
     3301 ggtatgtatt gaatgatgaa ataacacctc cacttaatta cataaggatt ctttaaaaca
     3361 atgaatttta aaaaaacaat aatcatttcg gtaggtttga tatttatttc attctctttt
     3421 gtggcaaagt gctctcaact caaaaatttg aataattact cagtgatgct ttgtggaaaa
     3481 gtgtcaaata atatcctgga tgatattggt ggttataaag aaagagataa aaaa
//
LOCUS       DAZFFG010000241         3514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFFG010000241 DAZFFG010000000
VERSION     DAZFFG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.242"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..2476
                     /locus_tag="ACHJ7J_004941"
     CDS             <1..2476
                     /locus_tag="ACHJ7J_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HFO5003030.1"
                     /translation="SNASRNVTFVADVRTAQIADLEVIKDGSVADGAMANTLQVKVTD
                     AFGNALGGQTVSVTAGNGATVASTVTTQSDGTVEFSVTSQTAGVSAVTATINNHSLSQ
                     NVTFVPGDASQLTSTVETNKSNYTVGETITITVTLRDAFDNLVTGAASQLAANSVLTV
                     DGTDPSETGSWVESGGVYTTTRMATIAGTDQHANLQLQTWSDGVTSDRYDIQSGSPAQ
                     ATSTIATDKNAYTAGETITVAVTLKDAHGNLVEGGESLLSGDNVTVEGAVRSGEWSET
                     AGVYTATWSAQMAGDSHHATLTLPEWGSSKQSESYSIHSGAPVQANSAIRTDKSAYIA
                     GEPLTVTITLRDEFGNPALGLTSEVIESYIDNFAVGGATSDSMQWVEQNNGEYTIVWT
                     AWVAEENLVASLKLKTWAMEIKSSLYGIQPGAAAKTQSTIVADKTIYIAGDSITVTVV
                     LKDAQGNFITDGVAQLNEENVQVRNADSIQGNNWVYNGDGKYQRQYMAHFAEANLNAQ
                     LKMAGWSDANYSKNYTINRGEVSMFRSQLRIREVLVVAGADIPVSVLLSDEFGNPVND
                     GLELLTEDAVYLQNVEKKEGAKWVSVGEGRYERTYRAYKEGENLNSYLHINGWYVDGQ
                     PSYTILPFVEVESLSVNGVRFRATDGFPETGFDGAKFTLLLTHNMRNTDYNWTAGIYG
                     INVDSNGEVTLSLLIRSEVTITGKPKNGKGNDVVFKFKIKKWFTSLGAASSNTWDIIN
                     ASCSYGQMPSSLELAQRPSGGVVPRKVGTLWGEYGNLKTYGNAFSGTDYWTTTQLLGV
                     HEKFNPETGISELGTGKSSGLCVEYY"
     gene            complement(2528..>3514)
                     /gene="eilA"
                     /locus_tag="ACHJ7J_004942"
     CDS             complement(2528..>3514)
                     /gene="eilA"
                     /locus_tag="ACHJ7J_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFO5003031.1"
                     /translation="KIPNINFKSINTQQMPSLDSAVMYMNGRMEMYRYTPESLKRALV
                     IFRDCVSTQPQNTLPYCCLAECHISLALLGISEQKQAITTAVTSIEKALEINPSNSQA
                     LGLLGLISGLKDEHSVSNVLFKQAHLLKPNSPDVYYYQSLLYFLNGDLARAFNLIEKS
                     IALEPNKMGISILKLIILYYTSPLDNAISFALNLNSQNTCNNPIITSILAMFMALKGH
                     NDKAKSLLLKLEPEHGLDYTCVNSLYTKFLIYGASIKNDIMKLLANINTNKINGVILP
                     LIYTVYGKKEYEKRWQQLIKENDLWSNVLLHDPRLLSVKNEFNTIGVMRTSA"
BASE COUNT         1016 a          674 c          878 g          946 t
ORIGIN      
        1 cagcaacgcg agccggaacg tgacgtttgt tgccgatgtc aggacggcgc agatcgctga
       61 tctggaggtc attaaggacg gctcagtggc agacggcgca atggcgaaca cgctgcaggt
      121 gaaggtcacc gatgcgttcg gcaatgcgct tggcggacag acggtcagcg tgacggcagg
      181 caacggcgca acggtcgctt cgacggtcac cacgcagtcg gacggtacgg tggagttcag
      241 tgtcaccagt cagacggcgg gagttagcgc agtcacagca accatcaaca atcatagcct
      301 gagccagaac gtcacctttg taccgggtga cgcatcgcag ctcacttcaa ccgttgaaac
      361 aaataagtct aactacacgg ttggagagac catcaccatc acggtaacgc tccgggatgc
      421 gttcgataat ctggtaaccg gtgcagcttc acagttagcc gctaatagtg tgctgacggt
      481 ggatggcacc gacccgtcag aaacgggaag ctgggttgaa tcaggtggcg tgtataccac
      541 aacccgaatg gcgacgattg ccggtaccga tcagcacgct aacctgcaac tgcagacctg
      601 gagtgatggt gtaacgtcag atcgctatga catacagtcc ggttctccgg cgcaagccac
      661 atcaactatc gccacggata aaaacgcata caccgctggc gagacgataa ccgtcgccgt
      721 gacgctgaaa gatgcgcacg gtaacctggt tgaagggggc gagtccttac tgtctggtga
      781 taacgtaaca gtggaaggag ccgtacgttc aggagagtgg tctgaaactg ccggtgttta
      841 tactgctaca tggtcggcgc aaatggcggg agactctcac cacgcgacgc tgacgttgcc
      901 cgagtggggc agcagtaaac aatcagagag ttattccatc cacagtggtg ctccggtgca
      961 ggcaaattct gctatcagga cagataaatc ggcatatatc gccggggagc cattaactgt
     1021 cacaataacg ttgcgggatg agtttggtaa ccctgcttta gggctgacat cagaagtcat
     1081 cgagtcttat atcgataatt tcgcggtagg tggcgcaact tccgattcta tgcagtgggt
     1141 cgagcagaat aatggtgaat ataccattgt ctggacagca tgggttgccg aggaaaatct
     1201 ggttgccagt ctgaaattaa aaacatgggc aatggagatt aagtcctcgt tatacggaat
     1261 acaaccaggt gcggcggcaa aaactcaatc cacgattgtc gcggacaaaa cgatatatat
     1321 tgctggggat agcataacgg tgaccgttgt tcttaaagat gcccagggta actttattac
     1381 tgatggtgtc gcgcagctta atgaggaaaa cgtacaagtc aggaacgctg attctattca
     1441 aggcaataat tgggtttaca acggcgatgg taaatatcaa agacagtata tggcgcattt
     1501 tgcggaagcg aatttgaatg cgcaactgaa gatggctggt tggagcgatg ccaattattc
     1561 aaaaaactac actattaaca gaggtgaagt aagtatgttt cgttcacagc ttcgtatacg
     1621 cgaagtttta gtggtagctg gggcggatat acctgttagt gttttattat ctgatgaatt
     1681 tggtaatcct gtgaatgacg gtctggaatt gctgacagag gatgccgtgt acttacaaaa
     1741 tgtggaaaag aaagaagggg ctaaatgggt atctgtgggt gaaggcagat atgagcgtac
     1801 ctatagggcc tacaaagaag gagaaaactt aaactcatat ctacatatta atggttggta
     1861 tgtagacggg caaccgtctt ataccatcct accttttgtc gaagtcgagt cgctgagtgt
     1921 taatggtgta agattcaggg cgacagatgg ttttccagaa acagggtttg atggtgcaaa
     1981 attcacgctg ttactaaccc ataatatgag aaatacggat tataattgga cggctgggat
     2041 ttatggaatt aatgttgaca gtaatggaga agtaacatta tctctgctaa taagaagtga
     2101 agttactatt accgggaaac ccaagaatgg taaagggaat gatgttgtat ttaaattcaa
     2161 gattaaaaaa tggtttacta gtttaggtgc tgccagtagt aatacctggg atatcataaa
     2221 tgcttcatgc agctatggtc aaatgccgtc atctttagaa ttagcgcagc ggccaagcgg
     2281 tggggtagta ccgcgaaaag taggtacgtt gtggggcgaa tatggtaatt tgaaaactta
     2341 tgggaacgct tttagcggta cggattattg gacaaccact cagctcttag gggtacacga
     2401 gaaatttaat cctgaaactg gtatatcaga gcttgggaca ggaaagtctt ctggcttgtg
     2461 tgtagagtat tattaacgaa gtaatcacta aattgccctg catcagcagg gcaatttata
     2521 ttagttctta cgcagaagta cgcatcacac caatagtatt aaattcattt ttcacactga
     2581 gcaaacgggg gtcatgaagc aaaacatttg accacaaatc attctcttta attaattgtt
     2641 gccagcgctt ttcatattct tttttaccat acacagtgta aattaacggc aaaataacac
     2701 cattgatttt gttggtgtta atatttgcca gtaacttcat gatgtcattt tttatagatg
     2761 cgccatagat cagaaatttc gtataaagcg agttaacaca ggtgtaatca agaccatgtt
     2821 caggttcaag tttaagcaat aaacttttgg ctttgtcatt atgtcctttc agtgccatga
     2881 acatagccag aatagatgta ataattggat tgttacaggt gttttgactg ttaagattta
     2941 atgcaaaaga tatcgcgtta tcgagtggtg atgtataata aagtataatt aatttaagaa
     3001 tgcttatgcc cattttattg ggttcaagag caatgctttt ttctattaaa ttaaatgctc
     3061 ttgcaaggtc gccgttcaaa aaatatagca gcgactgata ataataaacg tctggcgaat
     3121 taggtttgag taaatgtgct tgtttaaata gcacattaga cactgaatgt tcatctttta
     3181 gcccactgat taaacccagt aaacccaatg cctgagaatt tgaagggtta atttccagag
     3241 ccttttcaat ggaggttact gccgtagtta ttgcttgttt ttgttcactg attccaagga
     3301 gtgctaatga tatatggcat tccgccaggc aacagtaggg caaggtattt tgcggttgcg
     3361 tactcacaca atctctaaat attactaatg ctcgtttcaa actttctggt gtataacggt
     3421 acatttccat cctgccattc atatacataa cagcagaatc cagagatggc atttgttgtg
     3481 tattaatgga tttaaaatta atgttgggta tttt
//
LOCUS       DAZFFG010000242         3514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFFG010000242 DAZFFG010000000
VERSION     DAZFFG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.243"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            295..3498
                     /locus_tag="ACHJ7J_004943"
     CDS             295..3498
                     /locus_tag="ACHJ7J_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5003032.1"
                     /translation="MTSFLTGEAIAIGSKANAAKEQSVAIGADTKATGWGSVVIGGDD
                     IQPVHGKTYGRLIVPNKYSPATASGTGSVAIGSRTLSSGELSLALGSIASAKGDASTA
                     IGATAEAQDELSTAIGFNALSTARSALALGSESSATSESALAFGSRASAGKSSSIALG
                     TDAVVSVGDNNVALGAGSQDKAATSVSSASVRSSDGRLVTYQGFAASDPSSVVSVGAQ
                     GKERQIVNVAAGAVTENSTDVINGSQLYSVAKSLQDDINAVTNAGFALKDQGNNEVRQ
                     VSGSAIQITGDDNITTTATDSDDNKSLKISLNDDLTVGHDKAGSITVNNAAGNSSISL
                     NGGDGTISLTSPAASGDTSTTATLGVTTGQGGLTDADGATKTRLIYTPDGSSEPEELA
                     SMNDGLSFVGDDGNLINKKLNETLSVTGGAGADAEMTDNNIRVDNQEGGLWVRMTRNL
                     RDLASAVFGNGDNITTITDNGMSVTTGTGDNVRTVSLGKEGLNNGGNIITQVASGLNG
                     KQLSEADGDTLTNAANIGDLKSALQDTTSALVNSGYGVQADDGNKVTRPLGEHVSIAG
                     DGRNITTSVQDGKLLVQLSEHIDTGKNGSVTVGDTVITDEGLSITGGPSVTKNGINAG
                     GQVISGVQDGVDAHDAVNVSQLNQQINNATAATTWSLKTESSDASVAVSSQTVEVKHG
                     LNTRISAVQTDDKGNYSYEIDVTGIPVEYTDSKGNPLVNIGGKFYTRTEDPDSGNITL
                     SPAEPARVRISSDQPLVLTNVADGAVTQESVDAVNGGQLASAGNIVAGKNVVWNNGKA
                     TLSPDTFSELTTAGGSNRTTTGTVQAVPDNVADAVNMLNREGTKYFKASSSGPAAKAE
                     GRNSVAIGQSAISRAENSIAMGNGAEVSGDAAAGSVAIGSGSRAGRAHTGNYSLNGQA
                     VAGRTGSNTPVMSFGQAGQERQLQHVAPGVLSAESTDAVNGSQLYAVHRQTEGNTQTI
                     NNLGKKFDHLNDRVDELNRDIRGVGASAAAMSGIPQAYLPGKSLMGLGVGGYGGESAV
                     AIGVSRISDNGKLIMKMNAGQNSRGNFSVGAGVGWQW"
BASE COUNT          942 a          802 c          991 g          779 t
ORIGIN      
        1 tccaatatac tcaggtatct ggtaaagtat taagggatgc ttatgctttt atttaagaag
       61 aacttaatta cagtttctat actgttatcc atttcatcat ttctgacata tgccaatctt
      121 gtcgaagttc ccgtgtatgt tgaagaaact atgggaccag actggcaaag tatgacaata
      181 ggaactactg aaacaaggct acaaggaaac ggtgtttcat tcgatcatgt tccacctgta
      241 gctaaaggta gaaggtctat agctataggt actagtgctg tggctggaga tcagatgacc
      301 agtttcttga ccggagaggc aattgctatc ggctctaaag ctaatgctgc aaaagagcaa
      361 tctgtggcaa ttggtgctga taccaaagcc actggctggg ggagcgtcgt tatcggagga
      421 gacgatatac aacccgtaca cggaaaaact tatggtcgtc ttattgtccc caataaatat
      481 tcacctgcca ctgcctcagg aacaggttct gttgccattg gcagccgcac gctttccagt
      541 ggggagttat cgctggctct gggctctatc gcctctgcga aaggtgatgc ctccacggct
      601 atcggtgcca ctgccgaagc acaggatgaa ctctccaccg ccattgggtt taacgccttg
      661 tccacggcca gaagtgcact ggctcttggc agcgaatcat ctgcaacctc cgagagtgcc
      721 ctggctttcg gcagcagggc cagcgccgga aaatcatctt ccattgcatt aggcactgat
      781 gcagtggttt cggttggcga caataacgtt gctctggggg ccggttcaca ggataaagcg
      841 gccacaagtg tgagcagcgc cagtgttcgc tcatcggatg ggcgtctggt tacctatcag
      901 ggctttgccg ccagtgatcc atcctctgtt gtttctgttg gcgctcaggg gaaggaacga
      961 cagattgtga atgttgcagc cggtgccgtg actgagaaca gtaccgatgt cattaacggc
     1021 agtcagttgt attctgtggc caaatcgctt caggatgaca taaatgctgt cactaatgcc
     1081 ggatttgcac tgaaagacca gggaaataat gaggtcagac aggtgtcagg cagtgccatt
     1141 cagatcaccg gtgatgacaa tatcaccacc accgcgacgg acagtgatga taacaaatca
     1201 cttaagattt ccctcaatga tgaccttaca gtggggcatg acaaagcggg gagtataacc
     1261 gtcaataatg ctgcaggtaa cagcagtatc agcctgaacg gaggggacgg gacaatttct
     1321 ctgacctcac ccgcagcttc cggtgataca tccaccacag caacacttgg tgtgaccacg
     1381 gggcaggggg ggctgacgga tgcagacggc gccacgaaaa cccgactgat ctatacacct
     1441 gacgggagca gtgaaccgga agaactggcc tccatgaatg acggtctctc ttttgtcggt
     1501 gatgacggaa atctgattaa taaaaaactg aatgaaacac tgtcagtgac ggggggagcc
     1561 ggcgcagatg ctgaaatgac cgacaacaac attcgtgttg acaaccagga ggggggactc
     1621 tgggtccgta tgacccgtaa tcttcgtgat cttgccagtg cggtcttcgg aaacggagat
     1681 aacattacaa cgataactga caacggtatg tcggtgacaa ccggtaccgg agataacgtg
     1741 cgtaccgtct ctcttggtaa agaggggctt aataatggcg ggaatattat cactcaggtg
     1801 gccagcggac tgaacgggaa acaactgtct gaagctgacg gcgacacact gacaaatgca
     1861 gccaatattg gtgatctgaa atcagccctg caggacacaa cttcagcact ggtgaacagt
     1921 ggttacggag tgcaggctga tgacggaaac aaagtcaccc gtccactggg agaacatgtc
     1981 agtattgccg gtgatggccg taacatcacg accagtgttc aggacggaaa actgctggtt
     2041 cagctttcag aacatattga taccggtaaa aatggcagtg tgacagtggg cgatacggtg
     2101 ataacagatg aaggactgag cattacaggc ggaccatctg tcacaaaaaa cgggattaat
     2161 gcaggcggtc aggttatatc tggtgtacag gatggcgttg atgctcatga tgccgtcaat
     2221 gtatctcagt taaaccagca aattaataat gccactgctg caacaacctg gtctctgaaa
     2281 acggaaagca gcgacgcctc tgttgcagta tcgtctcaga ctgtggaagt aaaacatggc
     2341 ctgaacaccc gtatatcggc tgtacagact gatgacaaag ggaattacag ctatgaaatt
     2401 gatgtgaccg gtattcccgt ggaatatacc gacagcaaag gcaacccgct ggtgaatatt
     2461 ggcggcaagt tttatacccg gactgaagat cctgacagtg gcaacatcac cctttctccg
     2521 gcagaaccgg ccagggttcg tatcagcagt gatcagccac tggtcctgac aaacgtggcg
     2581 gacggtgcag taactcagga gtctgtggat gcggtaaacg gagggcaact ggcgtcagca
     2641 gggaatattg ttgccggcaa aaatgtggtc tggaataacg gaaaagcgac cctttctccg
     2701 gatacgttca gtgaactgac cactgccggc ggaagcaacc gtaccacgac aggtaccgta
     2761 caggccgtac cggataatgt ggcagatgct gtcaatatgc tgaaccgtga agggacaaaa
     2821 tatttcaagg catcctcatc cggaccggcg gcaaaagcag aaggcaggaa cagcgtggcc
     2881 ataggacaaa gtgccatttc ccgtgctgaa aacagcattg caatggggaa tggtgcagaa
     2941 gtcagtggtg atgcggctgc cggctctgtt gctattggca gtggttcacg ggccggacgg
     3001 gcacacaccg gtaattattc gctgaatggt caggctgtgg ccgggcgtac gggaagtaat
     3061 accccggtca tgtcattcgg gcaggccggt caggaacgcc agcttcagca tgttgcaccg
     3121 ggggtattat ccgcagaatc aacagatgcc gtgaacggaa gccagttata tgccgttcac
     3181 cgccagactg aaggaaacac tcagacaatc aataatctcg ggaagaaatt tgatcatctg
     3241 aatgacaggg tggatgagct gaaccgggat attcgcggag tcggcgcatc agcagctgcg
     3301 atgagcggta ttcctcaggc ttatctgccg gggaaaagtc tgatggggct tggtgttggt
     3361 ggatacgggg gtgaaagtgc cgtggcaata ggggtatccc gtatatcaga taacgggaaa
     3421 cttattatga aaatgaatgc agggcaaaat tcacgcggaa acttttctgt gggagctggt
     3481 gtgggctggc agtggtaacc cggggtaagg agta
//
LOCUS       DAZFFG010000243         3493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFFG010000243 DAZFFG010000000
VERSION     DAZFFG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.244"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(233..1552)
                     /gene="digH"
                     /locus_tag="ACHJ7J_004944"
     CDS             complement(233..1552)
                     /gene="digH"
                     /locus_tag="ACHJ7J_004944"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO5003033.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSN
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1683..3218)
                     /gene="gadC"
                     /locus_tag="ACHJ7J_004945"
     CDS             complement(1683..3218)
                     /gene="gadC"
                     /locus_tag="ACHJ7J_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO5003034.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3374..>3493)
                     /locus_tag="ACHJ7J_004946"
     CDS             complement(3374..>3493)
                     /locus_tag="ACHJ7J_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003035.1"
                     /translation="GFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          914 a          837 c          869 g          873 t
ORIGIN      
        1 tactgttctt cagaagagta tagcccatcg taattatttt tcggtgactg cgaatatcgt
       61 atggtttttc atattcatac atttttatta gggggattat ggctgtttaa ctaagcgtgg
      121 ttaattgcgt ggttaatttg actttagtaa gcatgattgc tagtgggata gttcaagagg
      181 gtaacaagcc agtgggtaaa gcaccggctt gttacaaagt aaaaatggga gtttaactgc
      241 cccaacgact ttgtagatag ctgaccgctt gttgagtctg cggtttattc agatagtcct
      301 cacggaacaa gatggtgccg ctaatttcgg gtaccgcatc gttaagatcg agctgctttt
      361 tcagttccgg tacgccgccg ttaatcatcc agtctggctc tatctttgaa ggttcaccca
      421 ctttatagaa ggcgataccg atatacaggc gggtcctggt cggtttaacg acatccgccc
      481 accattttgc caacacgtca taacgcgcgg cactccgtga gaacggccag taaatttggg
      541 gagcaatgta atccagcaat ccttgttcca cccatcgacg ggtgtcagcg taggattcgt
      601 cataggctgc cgcgcctcgg gtatcggaac cgagcggatc gtgtgatcgg ttacgccaca
      661 cgcctgccgg gctaacacca aattcgactc ccggcttaat gcttttaatg gtgtgcgata
      721 cctttgcaat taactgctga gtattgttgc gccgccagtc tgcttttgac gcaaatgcgc
      781 ctccgtattt gcggtacgtt tcgttatcat ttagccgtga acccggtgac tcagtataga
      841 aatagtcgtc aaactgcacg ccatctaccg gatagcggga aaccacttct gcgactattg
      901 atgtgatcca gtcctgaacc tcagggatgc ccgggtcgag gacaaagcga tcgccggacg
      961 ttctgatcca gtcgcggtgt tgcacataga cgctcgccgg ttgttgagac agggtgctat
     1021 tcagttccct gatagtaccg ggcttcgtat taaccgatac gcgatagggg ttaaaccagg
     1081 cgtgtacttt catcccacgc ttgtgggctt cgtcgagcat aaattgcagc ggatcgtaac
     1141 ccggattttc accaatctta ccggtcataa gatcggacca cggcaaaatt tttgaaggcc
     1201 acagggcggt accgtccggc ttgacctgga aaaagaccgt gtttataccg agacgttgca
     1261 gatgatccag tttgtcgatc atcgcctgtt gttgtacacg ggcccggctg gtggggttac
     1321 taatgttaac cgaggaaacc ggtggccagt cgaggcgaga caccgtggcc agccagatgc
     1381 cacgcatcgg ttgtgacgat tgttgcgtcg tggctggtgg atttgaacct gctggtggtg
     1441 tcaccatgga ctctggaggc gtgcttttac aactacacag taaaagtgcc agtgcaacca
     1501 gtatcgctgg tcttctaatc gctaatttct cgtttcggga gcagatatcc atatgtgctg
     1561 gtttccggta aacagatgtg cgctcattct cgtacttatc ccggttaagt caatacgaca
     1621 gcaagcacga aaaagggagc gatgaattat cgctccctta tcttataacc attcagacat
     1681 ggttagtgtt tcttgtcatt catcacaata tagtgtggtg aacgtgcacg cgggtgcagg
     1741 aagaagtgac cttttggtgc gttctgactg ttgattggct ccagagtgac gccggtattc
     1801 gctttgcctt tacgatcatg aacagcatag agaataaagg gcagggcaag taccaccagg
     1861 aaactaacga ccagtaattc aacatacata tcggtagagt caccctggat gttatccggc
     1921 ggcaggaagg aaacaataaa cgccataatt gaagtcagca gaccgacaat tgccacgacc
     1981 agtttcaccc ctttaccacc agggatatta aatgtgcgtt ttaagtcagg atgtttaaga
     2041 accaacacaa tgtagccaat aaacagcatg aaataagcac acagataaat caccaccgtc
     2101 agcgccagtg cgatcaggaa ggacatgttg ttaccgccac cggtattggt gaggatgatc
     2161 aacgcgatag aagtaatcac cagctgcgaa atgaccagcg ttaccggtac gccatttttg
     2221 ttcattttag cgaatgccgc tggcagcagg tttttctgcg ctgtgacata catcccgcga
     2281 gaaggaccaa caatccagga ggcgatttcc gccagaacac ccagcaacag cagtgcggag
     2341 atcacgcgaa ccgtccactc aatttccggt gccacatgag acatcagaac ggtaaaggtt
     2401 tgcattaccc ctgcggagag gttgatttca ttacccggaa tgaccatcgc aatagacaaa
     2461 ccaccaacag agcttaagca gattgccgcc accatcagca gtaacatagc cagtggatag
     2521 tcgcgccctg ggttgctcat ttcattgacg tgggttgcgg atgcttctac gcccatataa
     2581 ctcaaaatga aggcaacaaa tactaccagg gtgcccactt tagagaagtc agggaagaag
     2641 gtcttcgaat ccatttcgat agcaacgggg gcaccggagt gcagataaat agccgctaat
     2701 gcaatcaaaa taaatgcagg taacaggatg ccggcgaaga agccaacttt agcaattcgc
     2761 gccgtgtatt tcgtgccacc aaactgcgtt aatgccagcg cccaaagaat gatgagtgct
     2821 gcaatagttt tggtaatggg gtcttcattc agcgctggcc atttcaggat gtaggagagt
     2881 gcccctaaca cgaaatagag catcggaata aaaccaatgg cgatttgcag atagccaaat
     2941 gagatcgctg caaatcccca tctcggcccc agagtatttg atacccaggc gaagacacca
     3001 ccttcttccc agccgtcgac ggtggccatt tccgcagcac aaagtcccac gggaataaac
     3061 cataaaatcc cgcctaatag caggaagaag actaatgaaa agcccgatgt tgcgaaggta
     3121 gggtattcat aaacagccat taccatcgat gccgttatgg caaaaaagcc gagtaatgtg
     3181 agctgcttag ctttacctgt ctgtactgat gtagccatat tatcccccta aaacggtatt
     3241 cctgtcggaa ccgcacctgt tttgttctga agcgtattca gaacaatatt ttccgttgct
     3301 aatgccagtg aacagacttt ggaaattgtc ccgaaacggg ttcgtttcgg gacaccgtta
     3361 ccgttaaacg ttatcaggta tgtttaaagc tgttctgttg ggcaataccc tgcagtttcg
     3421 ggtgatcgct gagatatttc agggaggctt tgtagtcttc cagcaacagt tcagcaaagt
     3481 ccatttcgaa gcc
//
LOCUS       DAZFFG010000244         3485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFFG010000244 DAZFFG010000000
VERSION     DAZFFG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.245"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            536..1765
                     /locus_tag="ACHJ7J_004947"
     CDS             536..1765
                     /locus_tag="ACHJ7J_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5003036.1"
                     /translation="MSYPVQKKIKTGENVLRAAVERINWVFDTFAEICLSFSGGKDST
                     VLFHLVADIARRKKRRFSVLFIDWEAQYLCTVRHVQKMRELYMDVTESFYWVALPLTT
                     VNGVSQFQPEWICWESGVEWVRQPPDYAVTAPDFFPFYQYAMTFEEFIPAFSSWMARS
                     KRGLAVLTGVRADESLNRFMGLASQHKLRYADDKPWTTASQDGFYYTMYPLYDWKTRD
                     IWIYNAKTHAIYNPLYDLMFRAGIPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMC
                     VRVSGAASGALYANESGAYFALRKRISKPDHHTWRSYAMFLMNVMPEKTAEHYRNKIA
                     VYLRWYQTHGYPDDIPDEQDNDLGSRDVPSWRRICKTLIKNDFWCRTLSFSPNKPRHY
                     ERYLQRMKERRKEWGIL"
     gene            1750..2388
                     /locus_tag="ACHJ7J_004948"
     CDS             1750..2388
                     /locus_tag="ACHJ7J_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO5003037.1"
                     /translation="MGDPLTPEVLISMIRQYFSQVRTEEETIQALNHLRRVLHDISPF
                     AREPVDCVLWVKADEVVANDYNPNVMAPGEKRLLKQSLEQDGFTQPVVVSEEKDHYLV
                     VDGYHRQLLGRGSGAEKRLRGWLPVTCINPERKGQAARMAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWSDERIGTELGMDQDEVLRLKQISGLTELFQDEDFSPAWTVR"
     gene            2421..2633
                     /locus_tag="ACHJ7J_004949"
     CDS             2421..2633
                     /locus_tag="ACHJ7J_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5003038.1"
                     /translation="MSTQKRRLEAVEDLIALKLEVAGCWRRASARWLTVMGEADITDA
                     QREWLLRRREYCMSQIMPPGKVNSEK"
     gene            2781..3035
                     /locus_tag="ACHJ7J_004950"
     CDS             2781..3035
                     /locus_tag="ACHJ7J_004950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003039.1"
                     /translation="MSGNNLSEECFDYLCMDVNYDTVNEIIERLMVVCETPADGLHVV
                     AVVANFLMKHYGIDVFESCLAEGNGICLKLISVSEEGHIH"
     gene            complement(3193..3398)
                     /locus_tag="ACHJ7J_004951"
                     /pseudo
     CDS             complement(3193..3398)
                     /locus_tag="ACHJ7J_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          947 a          628 c          912 g          998 t
ORIGIN      
        1 atgtgataag aacaggagag gcagaaaatg cattaggaac cgacggggga aggaaaacag
       61 atttctgtac cggatgcctg atatgttaat catcacaaaa acagacgagt tatctcaggt
      121 aattcaccaa tgcgggacag agaggctgca tgaccatcag ggtgataagt cgtagtctgg
      181 cgcagagtct gatggaaatt tttgagatgt atccctgtat gtcagggatg tactgcagtg
      241 agggtcccgg catcttatta ctgtattgtg tgttaaatta cgtagtctgt ttatgtataa
      301 gatataatcc tgttttctgg catatccata ctgtacagtc atcatggcat tataagagat
      361 atgttgattg ttttgtgatg catttcaatt tttttgttgt tgttattgtt atatttttta
      421 aaaatatctt actcattgtg aaacgatttt tatcgtgcac tgtgcttgat tacatggttg
      481 ccagaggtgt ctctgtgtaa gtgatgtttc tgtatgttta ttgcattgag aacaaatgag
      541 ttatccggta cagaaaaaaa taaaaacagg tgaaaatgtt ctccgggctg ctgtggaacg
      601 gattaactgg gtttttgata cttttgctga gatttgtctg tctttttccg gaggtaagga
      661 ttccactgtc ttatttcatc tggttgcaga tatcgcccgc agaaaaaaac gacgtttttc
      721 cgtgttgttt atcgactggg aagcgcagta tttatgtact gtccgccatg tacaaaaaat
      781 gcgtgaattg tatatggatg tcacagagtc tttttactgg gtggccctgc ctctgacaac
      841 ggtgaatggg gtttcccagt tccagcctga atggatatgc tgggagtcgg gagtggaatg
      901 ggtccgtcag ccacctgatt atgctgtcac tgccccggac tttttccctt tttatcagta
      961 tgccatgaca tttgaagaat tcattccggc tttttcatcc tggatggcca gaagtaaacg
     1021 tggactggca gtgctgaccg gcgttcgtgc ggatgagtcc ctgaacaggt ttatggggct
     1081 ggcttcgcaa cataagctac gctatgcgga tgataaacca tggacgactg catcacagga
     1141 cggcttttac tacacgatgt accctttgta tgactggaaa acccgggata tatggatata
     1201 caacgcgaaa actcatgcca tttataaccc cctgtatgac ctgatgttca gagctggcat
     1261 tccattacgg aacatgcgtg tctgtgaacc ctttggccct gaacagcgta agggattatg
     1321 gctttaccat gttctggagc ctgaaacctg ggccagaatg tgtgtccggg tctcaggagc
     1381 agccagtgga gccctgtatg caaatgaaag tggtgcttat tttgcgcttc gaaaacgtat
     1441 atcaaagcct gaccatcata cctggcgcag ctatgcgatg tttttaatga atgtgatgcc
     1501 ggaaaaaacg gcggagcatt accggaataa aatagccgta tatttacgct ggtaccagac
     1561 acatggatat cctgatgata ttcctgatga gcaggataat gatctgggaa gtcgtgatgt
     1621 tccgtcatgg aggcgtatat gtaaaacgct cataaagaat gatttctggt gccggactct
     1681 gtccttcagt ccaaataaac ctcgtcacta tgaacgatat ctgcaaagaa tgaaagaaag
     1741 gagaaaggaa tgggggatcc tttaacgccg gaagtactga tatccatgat ccgtcagtat
     1801 ttcagtcagg tacgaactga agaagagacc atacaggctc ttaatcatct gcgcagggtg
     1861 cttcatgata tcagcccttt tgcccgggag cctgtggact gtgtgttatg ggtgaaagcg
     1921 gatgaggtgg tagccaacga ttacaatccc aatgtcatgg cgccgggtga gaagagactg
     1981 ctgaagcagt cactggagca ggatggtttc acacaaccag tcgtggtatc ggaagaaaaa
     2041 gaccattatc tggtggtgga cggttatcat cgtcagttgc tgggaagagg gagcggtgca
     2101 gaaaaacgcc tccggggatg gcttcctgtc acctgcatta atccggagag aaaggggcag
     2161 gcggcccgta tggctgcgac tatccgtcat aaccgggcca gaggaaagca tcagattaca
     2221 tcaatgtcag atattgttcg tgacctgtcc cgtctgggat ggtcggatga aagaattggt
     2281 acagagctgg gtatggacca ggatgaggtg ctgaggctga aacaaatcag tggactgaca
     2341 gagttgtttc aggatgaaga tttcagtccg gcttggacgg ttcgttaatg agatgaaaca
     2401 tagttctggt ggatgagttt ttgagtacgc agaagagaag actggaggcc gtggaagacc
     2461 ttattgcgct gaagcttgag gttgcaggat gctggcgcag ggcttctgca cgatggctga
     2521 ctgtaatggg ggaggctgat attacagatg cacagaggga gtggctttta cgacgacgtg
     2581 aatactgtat gtctcaaata atgcccccag gaaaggtgaa ttcagagaag tagccaggtg
     2641 aatgggaata acaaacctgt aaaaagatag ttcagtgaaa tactgttata tctctgacgg
     2701 cagagtcgtt gttatagtga tgattttgac tacgatatat atggcaaagt tacagataag
     2761 attatgagaa ggagaaatat atgtctggca ataatttatc ggaggaatgt ttcgattacc
     2821 tgtgcatgga tgttaactat gatacagtta acgaaatcat tgaacgatta atggtggtgt
     2881 gtgagactcc tgccgatgga ttgcacgttg tagctgttgt ggcgaatttt cttatgaaac
     2941 attatggaat agatgtattt gaatcatgtc tggctgaagg aaacggaata tgtctgaagc
     3001 tcatatcagt ttcagaggag gggcatatac attaaacgat ggcaggaaat atgttttata
     3061 ataatatgca atacattagc gctgtggtat tgctgtccct acagataaaa taacttatcg
     3121 gatagaatca gtacgctgag aaatttgata gtgtttgttg aattctgtat tgtaggagta
     3181 aggcatactt catttcaggc gcgtcgtgaa gtatgtagcg gtctttggga ggatggactg
     3241 ctcttcatcc cgttctgtca actgaagttt aagaaatgca atattggcag atatcgtcgg
     3301 ttcacattca gaagaacgtc tatgattttg ctttttactg cgccagttta gcgttgtgat
     3361 ttctgcaggc ggagtgcacg gactgcggca gcaacaccgt tgcttttatc aatattcaca
     3421 tcttcactgc taaatctaaa tatttccgcg tttgctataa aatgtagata tcgttacatt
     3481 tatca
//
LOCUS       DAZFFG010000245         3472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFFG010000245 DAZFFG010000000
VERSION     DAZFFG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.247"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1136
                     /locus_tag="ACHJ7J_004952"
     CDS             <1..1136
                     /locus_tag="ACHJ7J_004952"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5003040.1"
                     /translation="NYATGENNKILVNFINKTLLLRPFSIPLINAPKSTDSNLFQAAE
                     QSVGFSNILPIITVKENDNRKEWVMNGFRILMNQHMNKEVASFMKIGYNTFINEVNNL
                     NKRMGDLRNINQTNGVWARIMNGSGSSNDGYKDRTMHIQLGADHKKNINSAEIFSGIM
                     ISHTDTKSYGNSYKGETKSFGIGGYISTLHNSGIYFDMMSKYVHHENKYDLQFSSAGK
                     HNFHTYSLYAGAEVGYRYFVNDSLFIEPQAELVFGKVQGQTINWNNNVTNMIMHRGSS
                     HPLVGRTGIVSGKNFRGDNWSTTARIGINYEFDLRNGPEITTKDDFSSYISKGEKDKR
                     IIYNVGVNTKFNNNIRFGIDAERSVGGKYNTDLLINANFRYSF"
     gene            complement(1364..1545)
                     /locus_tag="ACHJ7J_004953"
                     /pseudo
     CDS             complement(1364..1545)
                     /locus_tag="ACHJ7J_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1796..>3472)
                     /locus_tag="ACHJ7J_004954"
     CDS             complement(1796..>3472)
                     /locus_tag="ACHJ7J_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5003041.1"
                     /translation="KNDGNGTAFSLEEGTSEAVKDADRSVFNGSAVLNGKTTLDIMNA
                     TFNGDISGHAGSHVELSRRSIWNMTKSSTLDSFRSKGGTLSLVTDNWSPKTLTVNTLH
                     ASSMNIAMGVSTADNTGDRIDILNKATGGHNTLDLSSLFEQTVTLKNDLTLASAPVGT
                     SHEYFSFASLNRGFTVYTPDTQVQEKDGRVYWQLKSHAGTTESQVSTDVSDDVTDTTS
                     PVAPDTGSTGSTEANGIVSEGNNSNGVSPSPDSPAENAGTMVNGSTLFKGADNTSLLK
                     KARAMFAAREFILSDSADRWTQVVDNSDADGGAWAMTGYSHGGYDDFSLNQSGLNVGF
                     RQSAAGNAWWGMGAEFYRGHSSTDDYRDDFSLWGVHVLAGKSFAGGLFVDGMTGYREL
                     SEDYTIQGELSDLSGRAKSHILTAGIRGGWKMHAAPFDMSITPTVSLNGARVDGNRLQ
                     GRERSVELHDGDALWLKAGVEAEKVSGNMTLKAGIWRNITLNDMPGMTLRDDWKARHY
                     DAEKADRYTVSFGFNGKLTEKLSVQAKVNSSFDGYFKTDAEGILGIRYDF"
BASE COUNT          977 a          815 c          719 g          961 t
ORIGIN      
        1 ctaattatgc tactggagag aataataaaa tattagttaa ctttatcaat aagacgttac
       61 tactcaggcc ttttagtatc ccattaatta atgcacctaa aagtacagat agtaacttat
      121 tccaagcagc agagcaatct gtgggtttca gtaatatatt acctattatc actgtgaaag
      181 aaaatgataa ccgtaaagaa tgggtaatga atggattcag gatattaatg aatcagcaca
      241 tgaataaaga agtggcctcg ttcatgaaaa ttggttataa tacattcatt aatgaagtaa
      301 ataatctaaa taaacgaatg ggagatctgc gtaatattaa tcagactaat ggagtatggg
      361 ctcgtataat gaatggctct ggttcatcta atgatggata taaagataga actatgcata
      421 tacaactagg agcagatcat aaaaaaaata ttaatagcgc agaaatattc agtgggataa
      481 tgatcagcca tactgataca aaatcttatg gtaattcata taaaggagaa acaaaatctt
      541 ttggaattgg tgggtatata agtacattac ataattccgg catatatttt gatatgatgt
      601 ctaaatatgt tcaccatgaa aataaatatg acttacagtt ttcttcagca ggaaaacata
      661 atttccatac atattcatta tatgctggtg ctgaggttgg atatcgttat tttgttaatg
      721 actcattatt tatagaacca caagcagaac tagtttttgg gaaagttcag ggacaaacca
      781 ttaactggaa taacaatgtt acaaatatga ttatgcatcg tggatcgtca catcctcttg
      841 taggacgtac tggtattgtc tcaggaaaga atttcagagg agataactgg agtacaacag
      901 caaggatcgg gataaattat gaatttgacc ttcgtaatgg acctgaaata acaactaaag
      961 atgattttag tagttatatc tctaaaggag aaaaagataa acgtataata tataatgtgg
     1021 gagtaaacac taaatttaat aataatatac gatttgggat tgatgcagaa cgttctgtag
     1081 gaggtaaata caatacagat cttcttataa atgctaactt tagatattct ttttaataag
     1141 aatgtcctat acacatattc tgtataaata caaatgataa aatatacagg ataaaagtac
     1201 atcttgtgga ggatgtcagc aatgtccctt gagttgctac tctgccaatg atgtctgtgt
     1261 cagcgtggtc aagtaatatt ggccacggtt ttacagtaag aacggtatct gttctctgat
     1321 tcttccagag tcaatccaga gcctgttaag gattctgtgt aaaccactga ttcaacacat
     1381 agctgaacgt ttcacccagt ctggattttt tcgacagcgt gccatttttc tccaccatct
     1441 attcatgcag cgacgtcagt aacgctttgc ttcgttgcgc tgcctgactg caagacgctc
     1501 tgactctgct aatcctcgta tttcatcctc tatggagaac agttcacgta agagtaaacc
     1561 gactgccgta tgtagccatt aagccactat tggtaacgta ggtggcctgt tatggagcaa
     1621 aaacattatc cgcagaaccc cgcagatcat tttcaaattt tcaatttacg tttttcagta
     1681 accaccaaag cacaggaatc gctcgcagcc tgcaatccgg tcagaccatt acagactgcc
     1741 tgaaggggcc tgacgggccc cttcagaaga aaaacaccgg ggataacagt tattatcaga
     1801 agtcataccg gatacccagt attccttccg catccgtctt aaagtaacca tcaaaactgc
     1861 tgtttacttt tgcctgcact gacagtttct ctgtcagctt tccgttgaaa ccaaacgaaa
     1921 cggtatagcg gtcagccttc tctgcatcat agtgacgggc tttccagtca tcacgcaggg
     1981 tcattcccgg catatcgttc agcgtgatat tccgccagat acctgccttc agcgtcatat
     2041 ttccggagac tttttctgcc tcaaccccgg ctttcagcca cagggcgtcg ccatcatgaa
     2101 gctccacgct ccgctcccgg ccctgcagac gatttccgtc cacccgggca ccgttcagtg
     2161 aaacagtcgg tgttatcgac atatcaaagg gggctgcatg catcttccac ccaccacgga
     2221 tacccgctgt caggatatgg ctttttgccc tgccggacag gtcactcagc tctccctgaa
     2281 tagtgtaatc ttcagacagc tcccggtatc cggtcatacc atccacaaac agaccaccgg
     2341 caaaggattt gcccgccaga acatgcacgc cccacaggct gaaatcatcc cggtagtcat
     2401 ctgtgctgct gtgcccccgg taaaactctg cccccatccc ccaccaggca ttaccggcag
     2461 cagactggcg gaatcccaca ttcagtccgc tctgattgag gctgaagtcg tcatatccac
     2521 catgactgta ccctgtcatg gcccatgcac caccgtctgc atcactgttg tccactacct
     2581 gtgtccagcg gtcagcactg tcactgagaa tgaactcacg ggcggcaaac atggcacgcg
     2641 ctttcttcag aagagatgtg ttatccgcgc ccttaaacag cgtactgcca ttcaccatcg
     2701 tgcccgcatt ctctgccgga gaatcagggg aaggcgacac accgttgctg ttatttcctt
     2761 cagacacaat accattagcc tcagtgcttc ctgtactccc tgtatccggt gcaaccggag
     2821 aggttgtatc tgtcacatca tcactgacat ccgttgatac ctgagattct gttgttccgg
     2881 catgactttt cagttgccag taaactctgc cgtctttctc ctgcacctgt gtgtcaggag
     2941 tgtatacagt aaaaccccgg ttcaggctgg caaaagaaaa atattcatga gaagttccga
     3001 ccggtgcaga tgccagagtc aggtcatttt tcagtgtgac ggtctgctca aacagggagg
     3061 acaggtccag tgtgttatgt ccgcccgttg ccttattcag gatatcaatc cggtcgcccg
     3121 tgttgtcggc agtgctgaca cccatagcga tattcatact gctggcatgc agtgtgttta
     3181 ccgtcagcgt tttcggcgac cagttgtctg tcacaaggga gagcgtcccg cctttgcttc
     3241 tgaacgaatc cagtgtggag cttttcgtca tattccagat actccggcgc gacaactcca
     3301 catgcgatcc ggcatgaccg ctgatatcac cattaaaggt tgcgttcatg atatccagcg
     3361 tcgtcttccc gttcaggaca gcactgccat taaacacgct acggtctgca tctttcaccg
     3421 cttctgatgt gccttcttcc agcgaaaagg ccgtaccgtt accgtcgttt tt
//
LOCUS       DAZFFG010000246         3433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFFG010000246 DAZFFG010000000
VERSION     DAZFFG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.248"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..524)
                     /locus_tag="ACHJ7J_004955"
     CDS             complement(<1..524)
                     /locus_tag="ACHJ7J_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5003042.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDAGISAREAATRATDAADSARAASTSAG
                     QAASSAQEASSGAGT"
     gene            complement(589..1188)
                     /locus_tag="ACHJ7J_004956"
     CDS             complement(589..1188)
                     /locus_tag="ACHJ7J_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5003043.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(1259..>3433)
                     /locus_tag="ACHJ7J_004957"
     CDS             complement(1259..>3433)
                     /locus_tag="ACHJ7J_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5003044.1"
                     /translation="TELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELL
                     ETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPS
                     SGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTLRQRLFRC
                     VSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTAD
                     SGEYQVLARWDTPKVVKGGSFMLRLTVAADDGSERLVSTARTTETTYRFRQLALGNYR
                     LTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFW
                     FSEKRITDIRQVETTARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKSVFVEAVG
                     RASDDAEGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEWKDASDKWNAMW
                     GVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGN
                     QIFMNDVFLKRLTAPTITSGGNPPVFSLTSDGKLTAKNADISGSVNANAGTLNNVTVN
                     ENCTIKGMLEATQVRGDFVKAVSKSFPKKAGTWGNTETPDGTVTVTISDDHNFDRQII
                     IPPIIFNGIAYDYPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDM
                     PSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          722 a         1061 c          838 g          812 t
ORIGIN      
        1 gttcctgcgc cggaagaagc ctcctgagcc gacgacgcgg cctgtccggc tgacgtgctg
       61 gctgcacgtg ctgaatctgc agcatcagtc gcacgggttg ccgcctcacg ggctgatatg
      121 ccggcatcgc tggctgactt cttcgcggct gccgtgttct gtgccaccgc ggacgcgtta
      181 cgcgccacct cttccaccat cagctcaaaa cggcgcagtg cctccggacg ggcatcatcc
      241 tccgtcatgg caccgagaaa atcattcagc gtaccgggtc gggaatcttc atacacggtg
      301 atggttccgg catgtgaagg cggaaaacct tcaacaagca ggataacgct gtactggcca
      361 tactcaacat ccatgctgta acgcccggct tcatccggat tttctgaggc cagcgtgttc
      421 accaccaccg tggtgctgtt acgttttgct ttcagctgga ttgtgcagtt ctgtaccggt
      481 tttcctgtgc cgtctttcag tacacctgaa atctttactg ccatattcac cccacaaaaa
      541 agcccgcctg aaacggcggg ctgtcataac actgtgttac ctggctaatc agaacttata
      601 accgacaccc acgatgaaac cgtcagtgcg ccagtcgcca ctgccggagc cttcataagc
      661 aatatcaatg gccacggatt cggtcgggtt aaactgcacg ccagcccccc acgccagaga
      721 cgtgttgctg tggcgaccat catcacttcc ggtcagcaca tcgtgcgttt tccccttgtt
      781 gtcagttacg cggagataat ccccggagaa agtcgacaca cggctgtaag ccacacccgc
      841 catcgcatac gcgctgaacc attcattcac gcgcacagac ggccccgcca tcacgctgaa
      901 ccagcggtta cgcacggaat cttcatgcca gcgggtatcg ctgtaacggg taagctggcg
      961 attcctgtct cctgcatagc tgaatgacgt caccagcccc agtgtgtccg taaactcata
     1021 acggtatttc acgttaatcc cgttcagatc atcactgccg ggaacgttgg tccgggcatg
     1081 aagatatccc gcgctcagcg tggactgatg ttcagacgcc catgcaggcg caccggatac
     1141 ggccagacaa atggctgcgg acaaaatggc ggcataaagt ttacgcataa ttacctctcg
     1201 cttttctgca ataaaaaagg cgtcattcct gacgcccttt attggggtta taaatatttc
     1261 aacgaatact gataccggaa gccgcttttt tggtcacaat caccgtacag tcggtgatat
     1321 tgcctgcccc ctgattgcct ttctggaaaa tcttaaactc cagagtgacg ctaccaccac
     1381 cactaggcat atcaataact gcactgtaac taccgggaat ggccccttta gtttctctgg
     1441 atgcgattaa tacgccgttt ttgcgaactt caaaaccata acccgtgtat cgcgtgcctc
     1501 ctgggttatt tccgctcccc ggatagtcat acgctatacc gttaaaaata atgggcggaa
     1561 taataatctg gcggtcaaag ttatgatcat cgctgatggt gactgtaacc gtcccgtctg
     1621 gtgtttctgt gttaccccac gtaccagcct tcttagggaa tgattttgat actgctttaa
     1681 cgaagtcacc tctgacctgg gtcgcctcca gcatgccctt aatcgtacag ttttcattta
     1741 ccgtgacatt gttgagcgtc ccggcgttcg cattcacact gccactgata tccgcatttt
     1801 tagcggtaag ctttccgtct gatgtcaggg aaaataccgg tggatttcca ccactggtaa
     1861 tggtgggggc cgtcaggcgt ttgaggaaca cgtcgttcat gaatatctga ttgccctgcg
     1921 ccacaaacat cggcgtttca ttcccgtttg ccgggtcaat aaacgcgata cgattggcgg
     1981 caaccagaaa ctgactcagt ttgccttcct ccgtgtcctc catgctgagg ccaatacccg
     2041 cgacataatg tttgccgtct ttggtctgct caattttgac gccccacatg gcattccatt
     2101 tatcactggc gtctttccac tctttcgaaa actcctccag tttgctggcg ttatcctccg
     2161 tcagctcgac tttttccagc agctccttgc cgagatggga ttcggttatc tggcctttga
     2221 aaaaatccag ataaccttcc gcatcatcgc tcgcccgacc gacagcctcc acaaataccg
     2281 atttgccaac ggtgttcaca ctgcgaacat aaaaataata atcatggccc ggcctgatat
     2341 tgatactggc agctatccag tacagcgccg tgccaagata gcgggctgtg gtttcaacct
     2401 gcctgatatc ggtaatccgc ttttccgaga accagaactc aaactgtacc gtcggatcat
     2461 aaaccgcaag atgcggcgtg gcggttatct gaaaatagcc cggcgtcagc tcaatccgcg
     2521 acggcgctgc cggtgcggca atccggaacg ataccgacgc cggatcgccc tgctgtcccc
     2581 acgcatttac cgcccggacc gtcagcctgt agttccccag cgccagttgc ctgaagcggt
     2641 atgtggtttc cgtcgtcctg gccgtactga ccagccgctc actgccgtca tccgcggcca
     2701 cggtcaggcg aagcataaaa ctccccccct tcaccacctt cggcgtgtcc cagcgcgcca
     2761 gcacctgata ttccccgctg tctgcggtga cttcggcagt caggtgctgc accgcgggcg
     2821 gcgtgacacc attcaccgtg ccgctctggt caccgtcaaa gtgcgccccg ttatccacga
     2881 tggcttcttt ttccggtaca tgctgcacgg cggtgatggc atacgtgccg tcatcgttct
     2941 cacggatact cacgcagcgg aacaggcgct ggcgcagcgt cggcagcttc agcccccata
     3001 cgctgtatcc ggcaacgccg tcaggaacac ggctcacttt taccttcacg ccgtcggtga
     3061 cggactgaac ctccacgctg accggattgc cacttccgtc aaccaggctt atcagcgtgg
     3121 taccggagga tggcagcgtg atttcacggt cgagcgtcag cgtccgggtc tggctgttca
     3181 cagccagcac gcgcccgccg gtgctgatcc ccgcatagtc atcatcgcag atttcaataa
     3241 catcgcccgg tacatggcga agcccttcag cacccacgct gaagtccacg gtctgcgttt
     3301 ccagcagttc cgttttaatc agccacagcc cggcgcggtg tgcctgcccc cggctggtac
     3361 agccaaaggc atccatcttc gtgacgttac gaccgtaacg ggcaatggcc tgcgtgtcct
     3421 ccacaagctc tgt
//
LOCUS       DAZFFG010000247         3427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFFG010000247 DAZFFG010000000
VERSION     DAZFFG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.249"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(132..455)
                     /locus_tag="ACHJ7J_004958"
     CDS             complement(132..455)
                     /locus_tag="ACHJ7J_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO5003045.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGING
                     KWLRK"
     gene            complement(463..648)
                     /gene="ynbE"
                     /locus_tag="ACHJ7J_004959"
     CDS             complement(463..648)
                     /gene="ynbE"
                     /locus_tag="ACHJ7J_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO5003046.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(645..3284)
                     /gene="ydbH"
                     /locus_tag="ACHJ7J_004960"
     CDS             complement(645..3284)
                     /gene="ydbH"
                     /locus_tag="ACHJ7J_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO5003047.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWRITRQQLTVSDGRWSWPYVGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQVSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
BASE COUNT          846 a          918 c          779 g          884 t
ORIGIN      
        1 gtaatgattt gttatttaag ggaaggatat ataaggtttt tgtagaaata cccacggtaa
       61 ttaagcatgc taacgtcagt ttaaatactg taaaagatca ttaatagata cattttgtag
      121 ccaactataa tttatttgcg caaccattta ccattaattc cctgaacata ttgccccgac
      181 ttcgcgcgag caacaagctt ttgtccagcc agtttggcta tctctttagt ggatacatta
      241 ttttgctttg ccagttgttg gtaactatgg ttacgagctt cattaatatc ttttactaat
      301 gcctgtgttt cggcatccgt ttgtagcgcc accaggtaac cgttaagcgt ttcacctacc
      361 cgcccctgac ttctggcttc gttcagggtt aaagccattg cactgtcgct catcaggcca
      421 atggcccata cagctatgag taatgttctt ttcatcatca cctcaaaata gatcgctacg
      481 ggttttgagc aggtcttcaa cgtccttatc cgctttaatg atgatttcat gctcaatttt
      541 aacgttcata ttaatggtga ttggctcttt cggcgcagct acttcaatgc gaggcgtgca
      601 accaaccaac ataaaagagg acgcaaacgc ggctatcact attttcattg tttttcctca
      661 cactcttttc ctgatgggca gccagtttcc ggtagctggg cattctgctc cagccatacc
      721 tggagattat cgccaaagcg taaactgcgc cataacgtaa aaatattctc ttcatggtga
      781 taattaaggt ttaccgtacc gcttttacca tcgacgcgac tggtgcccga aatgttggcc
      841 tgcatggtta acacaccgag attatctaaa ttaatccttg ttgatgagcg actgatttcc
      901 atataacgca accagttgat ggcagttcca gcggtcatat tgtctttggc caccgcatct
      961 gcagtgtctt tatccagccg caaggtcatt ggcccgctat ttgccagcca accatctttc
     1021 actatccatt tttcattgtt cagccatagt ggcaaggccc cactgaatgc cccggacatg
     1081 gcaaattgtt tcggattgac ggcgctaacc agttcgctgg atgagagatt attcagccgc
     1141 aacagcgccg ggtcatgttg cggcatacgt aattgtttca tcagcacgtt accgcctaac
     1201 acatcgacgc taacatcagt caacagcaat ggttcttctt cagtccacgg ataacgccct
     1261 tgcaaatcag ccgtaatatt tttcgctgtc accagattaa tcacttcggc aattcgcaac
     1321 gtaacggggc cgcgagtccc cagatgccag gctccatcgg caaaacggaa aggcaggaca
     1381 aaatcgacac cgttaacctg attatctggc atccaggcac taccgccttt caacacgccg
     1441 tgtcctcccg cgcggaatcc ttgttcaggc gcagcagaaa atgcaacctg agcatacagt
     1501 tcaccatcgc gtaagttcat cttccagtcg ggtggcacca gcggctggaa tacagtcagt
     1561 gattgtttag gccaccaggc gttaccgcgc agacgaatac cgtcccagcg accatttacc
     1621 cgaaccgggc caatctcacc agcatgtaaa tcgcctttaa agaggaaata cgtcggatcg
     1681 cgcccatcga cgctaaattt taaggttgat ggcggtaaca cactgccgcc agtgaacagc
     1741 gtttgcccgg cgtccagtga cagcgcgccg ctgaaggagg gatgctgtgc gtcacgtacc
     1801 cagacgacgg gcttgtcgag aattaatcgc ggcttttcta ccgtcatcgt accgtattgt
     1861 aactggtcga aaccggtgga gagatcggtc agcgtaatcg tgctgtcatg ccactcacct
     1921 tttcctgcga catcccaggt ggcattcatc ggtgtaaaac tcccttttcc ccagtagcgc
     1981 cactgccagc ggccagcgtc agggagaaaa tcattcgcca gcccatccat atgcagcacg
     2041 aaatcgccca gttcattttc atgcgcctgc aaaatggcct gcaaacgtcc gtcaacacca
     2101 cgttgggtga cttttacacc cgctaaaggc cagcggattt catcgatgtc cagcgaatcg
     2161 atgactcttc cctttgaacg aagtaacgcg cctggctcaa aggtcagcgt tgggtcagac
     2221 agacttccac ttacctgcgc aggtaaacgg gcatataaaa tgaggtccgc ctgtttcgct
     2281 tcaccggtca gctgcagagg cagctggctg ttatccatgc ttaattttcc tgggccaaaa
     2341 ttaagcaccg cgttgccctt acccgcttgc ccctgggtca gcacactcaa tcgtccgctg
     2401 accagagcgt tctcaagccc tgcctgccag ttgtcgactt tgacacccag tcggccactc
     2461 aaaggaaaac ctacatacgg ccagctccag cgaccatcgc ttacggtcaa ttgttgacga
     2521 gtaattcgcc acggcaaatc gagcaacgga tcgccgttat cccgtgccag cacgatcaat
     2581 tgcccgctat tttcctgcca gtccagctcg gcatccacca gtgacggttc ctgcggcaag
     2641 tttaacgtcg cagtagcatg cccacttaca ggaagtccat ccggcacgag cggcatagta
     2701 aattccccca ccagtttaac cggcggctga ttttcaaacg cgacgacatc cagttcgctg
     2761 actgtaagtt gttgcccttt cagctggcct tgaaatttaa ctttttcgcc ctgataacgc
     2821 agttgctgga tatcagaggt taatgcgaga gagagttttc cctgccattc ctgccaggga
     2881 gaaaaaatca gtttatcgat attgatccag gtgttaggca gcatggactg ccactgcgcg
     2941 agggtttttg gagcagctgg cgattgctcc gtctgcggca atttcgccag acaagcagaa
     3001 tcaagttcta ccgtgccgac gttcaataac cagcggctgg gatgtgaaag gctggcgttg
     3061 gtgatatgcg caagctgaca atctcccacc agataacgga gatcgggaat gattaaacct
     3121 ttacgcgtaa tgcgtgggct ttcatctaat gcaatacgtg tcccgagcgg tagccagatg
     3181 cccgccagcg tgggaaccca cagcccgagc gtcatcagca gcgtcaacgg cacaagaata
     3241 atcagtaata acagcgcgag aacggcttta tatttaccca gcatgggtag ttaatatcct
     3301 gatttagcga aaaattaagc attcaatacg ggtattgtgg catgtttaac cgttcagttg
     3361 aaggttgcgc ctacactaag catagttgtt gatgaatttt tcaatatcgc catagctttc
     3421 aattaaa
//
LOCUS       DAZFFG010000248         3415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFFG010000248 DAZFFG010000000
VERSION     DAZFFG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.250"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(1050..1580)
                     /locus_tag="ACHJ7J_004961"
     CDS             complement(1050..1580)
                     /locus_tag="ACHJ7J_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5003048.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAVMAITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            2250..2981
                     /locus_tag="ACHJ7J_004962"
     CDS             2250..2981
                     /locus_tag="ACHJ7J_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5003049.1"
                     /translation="MNQPIHNDYWLSRFDSLLDSALAQHRAVSLIRVDLRFPEYMPVT
                     IMAPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATSLRYFWCREFGKMYGRKHY
                     HVILLLNKDTWCSLGDFSEPSSLATMIQEAWCSALHLEPWQGNGLVHFSRWTPSRKPA
                     SSDARPSSDVPSLSGGCSDTRKASGKKSGEAAVLWIKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRWSGHLLDGR"
BASE COUNT          905 a          697 c          725 g         1088 t
ORIGIN      
        1 tgatttgatg gttaatgctt acagataatc acactttctt atgaatagta taaatgagca
       61 tgccatagtc gtttgttcag agataaaatt tactgaatga gcttatgttg tggttatctg
      121 tacttaccag atacgacgac ttatatccct cacattatat ggcttaactt ttctggggta
      181 aagcgatgga gttacagacg ctgattacga tggtttactg tctcgttatc acgggatttc
      241 tgctgtactg aatacgtgaa caggaaaata actccagagt aaatctgctc tgtttttagg
      301 acagaaaact ccatgctggc tgtaaaagtg aaaaattaac acccagtatg tatggaagtc
      361 tgaagatgtt gagaatgtcg gtatgatact atatacacgt attttactaa gtatttttac
      421 taagcatttc agtaagtgtt atacacgtat tttctactaa gtgttttacc agacatacac
      481 gtattttcat aaacacttct acaagtgttt tcgttaagca ttgctataca catccagttt
      541 agtaaatctt tacgttaact aaatcagttt aatgctgctg taaaaccttt ctgtttaagc
      601 aatcaatctg ctattcgatt ttactgatgt tacgtttaac ctttccttag atttcgagtt
      661 tcgacgttag cttgagaaaa gtatctttac gtaaagccca gtgaattaac tcaaatccga
      721 ttgaggtaac ctgatgcgat ttactgtgag tttgcgataa cactgttaaa tggcttatgg
      781 atgattttgt aacggcgaga ttgataaggt gattaactgg ccctggtgtt ttttctcagc
      841 gtaaggtgat tatcttcatc ataaggctct tttcagaaaa agtgtgatga ttggttgtgg
      901 tgttttgatt cagatgagga tctgccaatg attatgatgt attgtaagaa ataacgcctt
      961 cagaatgttg gagcatgcac ttacacaaac tgtttttcta aaattaaggg aacagtttaa
     1021 agagtataaa ctgttcatct tcttcaggat taagctacat tgttgttatg actgacagga
     1081 ttgttttctt cccctgactc tgggcttaac agtccctggc gttgtagata ttcactgact
     1141 gacattccag aactacggat cctgtcagag attttacgaa attgatcctt ccattctgca
     1201 ggtaatttct taatttgtct gactttacag gctacactgt acggggttcg ccctagtttc
     1261 tctgccagcc agaaaaggtg tttaccaatt tcatcgtccg gtacatcctc cagaacatct
     1321 cgcaataaac tctcgtctgc ctcattccat gccacaccag catttgggaa acgaggatcg
     1381 atctctcttt ccttcttctc cttcttactt tgctcttttc gggatgatac agatacgaca
     1441 gaacgttctt ttaccgctgt aattgccata actgcattga gcagagatct gcgctccaca
     1501 tcgttcagca tttttccttc acagatcaaa tcattcagga tgtcaatgac tagattcaga
     1561 ctttcttctg ttagcttcat atttcagacc ttgaagtatg tagataatca gcacaattac
     1621 taatgtgata aatatcagaa gataatttac aggtaaaccg gaaaatacat ctgaagaata
     1681 aaggcctcag cttaacgttt cagccagttt gtgagctgat tgaggtacgg cgatgacatt
     1741 aacgggaatt actcccctat agctctgagc ttatttttca ccctggcaac atatggtggc
     1801 tactgcgcat ggttttggag tagatatctt actactcgta gaattgtgct tactggtcag
     1861 gccagcgcac aggcattccg tgcaatcaat agaacactgg ttttttagtc ttccgttacc
     1921 catcaggatg ttagtgcaga ttccggtgta ttcgatcagt tgttcggcga atcagcgatc
     1981 gatcacgatg cgatttcgta tgttagggat gctggtatga ttactcgctg aaaaataatg
     2041 tgaaaaggca gtttttcttt agacatttag ctcattcatg ctgttgtttt acgttttgct
     2101 gtcgtgtgca ggattatctt ttcgttacgg gacgattcat tccgttttaa tcaggagcta
     2161 ttggcgttgc tcattggtgg gatgccgtaa agttttaccg cggcgattaa tgatgtgaag
     2221 tcaatccaaa tcaacggaga tctctcatca tgaatcaacc aatacacaat gattactggt
     2281 tatcccgttt tgacagcctt ctcgacagtg ccttggcgca gcaccgtgcc gtctcgttaa
     2341 tccgggtgga tttacgtttc cctgagtata tgcctgtcac catcatggct cccgatccgg
     2401 attcagcggt gatttctcgt ttttttgcat ccctgaaagc caaaattcag gcttaccagc
     2461 ggaaaaaaag atgtgccaat cagcgtgtgc atgcaaccag cctgcgttat ttctggtgcc
     2521 gggagtttgg caagatgtat ggcaggaaac attatcacgt gatattactg ctcaacaaag
     2581 atacctggtg ctcgctcggg gatttcagcg aaccgtcttc gctggcgacg atgatccagg
     2641 aggcatggtg cagcgccctg catcttgagc cctggcaggg taatggactg gttcattttt
     2701 ccaggtggac gccttcccgt aaaccagcat cgtctgatgc tcgcccttct tccgatgtcc
     2761 cctctttgtc tggtggatgc tctgatacta ggaaggcttc aggcaaaaag tctggtgaag
     2821 ccgctgttct ctggatcaag cgtggtgatg tggaagcgat gcagaaagcc ctggagagag
     2881 cccgttatct cgtgaaaaat gaaacgaagc tgcatgacgg ttccgggcaa cgtaattatg
     2941 gttgcagtcg ttggtcggga catcttctgg atggcaggtg aagcctgtaa aacggcatcc
     3001 ggtgcctgag cttatgctac agggcgttgg ttgacgccct tagctcgttt cctgaaaaag
     3061 tttttctgaa gtaatgcaat gaattgccat ctacttccgt tcgcacactt gcttttcaca
     3121 ccccactctt tgcaacatcg agtataagtc gaaaaacgtt tggttgttct ggttatttgc
     3181 atttactgcc attcatagaa atgaacagat cgattggtag gactcaaatg ttatagcatc
     3241 tctcttctca tagtctgctt tgtgccaaaa gcggtcatag atacgagcat tttccgaaaa
     3301 ttaattccaa ttaaaacaag tatctacagt tcaacattat tgtccattag caaaaaaaac
     3361 ataaattcag gagaatttag tgagtaacaa gttcaattca cctttacaaa aataa
//
LOCUS       DAZFFG010000249         3326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFFG010000249 DAZFFG010000000
VERSION     DAZFFG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.251"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            64..999
                     /gene="lysR"
                     /locus_tag="ACHJ7J_004963"
     CDS             64..999
                     /gene="lysR"
                     /locus_tag="ACHJ7J_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO5003050.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLNTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGFTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLMVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(986..1678)
                     /gene="ygeA"
                     /locus_tag="ACHJ7J_004964"
     CDS             complement(986..1678)
                     /gene="ygeA"
                     /locus_tag="ACHJ7J_004964"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO5003051.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLMPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(1807..3225)
                     /gene="araE"
                     /locus_tag="ACHJ7J_004965"
     CDS             complement(1807..3225)
                     /gene="araE"
                     /locus_tag="ACHJ7J_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO5003052.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT          874 a          921 c          782 g          749 t
ORIGIN      
        1 atatcgattt atgtcgagtc tatgcaaaaa tgatatggat taccggattg cgagagagcg
       61 ctaatggccg ccgttaactt acgtcatatt gaaatttttc atgcggtaat gaccgccgga
      121 agcctgactg aggcggcaca cctgctaaac acctcacagc caaccgtcag ccgcgaactt
      181 gcgcgctttg agaaggtgat cgggctgaaa ttgtttgagc gcgtacgtgg gcgattacat
      241 cctaccgtgc aaggactgcg tctgtttgaa gaagtgcaac gatcctggta cggactggat
      301 cgaattgtca gcgctgcaga aagtctgcgc gagtttcgcc agggagaact gtctattgcc
      361 tgcctgccag tcttttcgca atctttttta ccgcagctcc tgcaaccctt tctggcacgt
      421 tatcccgatg tcagcttaaa tatcgtgccc caggaatcac cgctacttga agagtggctc
      481 tcggcccagc gtcatgattt agggttcact gagacgctcc atacgcctgc gggaacagaa
      541 cgtaccgaat tactctcttt agatgaagtg tgtgtgttac ctccgggtca tccgctggcg
      601 gtaaaaaagg tattaacgcc ggatgatttt cagggtgaga actacatcag cctttcccgt
      661 actgacagct atcgccagtt gctggatcag ctatttactg aacatcaggt taaacgacgc
      721 atgatcgtag aaacccacag cgccgcttca gtctgcgcaa tggtacgggc gggggtaggt
      781 gtttcggtgg ttaacccgct caccgcactg gattatgcgg caagcggttt aatggtgcgg
      841 cggttcagta ttgcggttcc gttcaccgtc agcctgatcc gccccctgca ccgcccgtca
      901 tcagcgctgg tgcaggcgtt tagtgagcat ttacaagcag gattaccgaa actggtcact
      961 tctcttgacg ctattttgtc gtcagctacg acagcataaa agcgacagca tcctcggcat
     1021 ggatcgccgc agtatcaaac acaggcaaaa cactgcgctc ttctggcatc agtaaaccaa
     1081 tttctgtgca gccaaaaatg acgccctgtg cgccctgttc tgcaaggcga gcaatcactt
     1141 gcgcataata agcgcgtgac gcttcggtaa attgccccag acacagttct tcaaaaataa
     1201 tctgattaat tttcgcccgt tcatccgctt caggaataag acaattgatg gaaaattgtt
     1261 ccgtcagccg cccgcgataa aaatcctgtt ccatggtgta acgcgtaccc agcagcgcca
     1321 cacgagtcat tccggccccg gtaattgcac gtccggtggc atccgcaatg tgtaagaaag
     1381 gcagagtgca acgagactct atagcatccg ccactttatg catcgtattg gtacataaca
     1441 caataccttc tgcgcccgcc cgctgtaagc caagcgcagc ctcagccaga atgtccccgg
     1501 ttttatccca ttctccgcga cgctggcact cttctatttc atgaaaatcg acgctatgta
     1561 gcagcacttg cgcagagtga agcccaccaa gccgctgttt aatgccttca tttatcagac
     1621 gatagtaagg aatggtggat tcccagctca ttcctcccag caaaccaatt gttttcattc
     1681 ctctctcctg atgtgtgtta ccgcaacaag gctgaataag gaacgagaca aacgcctcaa
     1741 cggccaggtg cccaatctct attaacgaaa aaagggccgg atgtacagca catccggccc
     1801 gtgaaatcag acgccgatat ttctcaactt ctcgcctgcc atcagtttgc gttcgatatg
     1861 ttccagggtg acatttttgg tttccggaat gagccagaaa gtgatgccca caaacgcaat
     1921 gttcagcgca gtgtagagcc agaacgtacc ggcagcgcca atgctgtcga gcagcgtcag
     1981 gaaggtcgcg ccgataatca tattcgacac ccagttcgtg gtggtcgaac aggtaatacc
     2041 gaaatcgcgg catttcagcg gctgaatttc agagcacagg atccacacca ctggcgcggc
     2101 gctcatcgca taaccggcaa tacacatcat cgtcatgcca acagagagcc aggacaagcc
     2161 actggaagcc gtaccgttat caaactgcat caggcaatag cccagcacca gagtgcctaa
     2221 cgccatcacg ctgaaaccaa ttttcagagc cggtttacga cctgctttat ctaccgtaaa
     2281 gaccgcaatg aaggtcgcga acataaaggt cagtcccacg accagagtcg caatcatctg
     2341 ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc ggcgcgtagt acatgatgat
     2401 gttcataccg gtaaactgct gcatcgcctg caacaacata ccgagaaaca cagcacgacg
     2461 gacgttacgg ttgatcttaa acagtgccca accgccctgt tttaacttca ggctttcacg
     2521 aatttcgttg agttcttctc gcgctttttc cgacgtatcg cgcagcatac gcaacacttc
     2581 ttccgcctca atatgacgcc ccttttccgc cagccagcgc gggctatttg gcaggaagac
     2641 caccagaata atcagcagga ctgctggtaa agcaagaacc cccaacattg cgcgccagtt
     2701 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
     2761 caactggtac atactgatca tcttaccgcg aacgttttca cttgccattt cagaaagata
     2821 cagaggagcg gtgtaagacg cgatcccgac agcaatgccc agcaccacac gagcggcgat
     2881 taacatctct acgctggtcg caaaagcgga ccctatagaa ccgagtacaa acaggatggc
     2941 ccccgccatc aggctgtatt tacgccccag gcggaacgac agccaaccat taaacagcgc
     3001 accaattgct gcgccgagca tcatgctact gaccacccat tcctgcaaac gactggtcag
     3061 cacaaagtga tcggtaatga acggcaacgc tccggcgatt acgccgatat caagaccaaa
     3121 taacaatcct gcgaccgcag cagctaccga aacaaacata ttcatacgcc gcgtatcccg
     3181 caaagaacgt ggcgttaaag cagattccgt attgatagta accatttttt cctgccagca
     3241 gagagtaaga catagtgaaa aaatacgtga acaactcaca caggtgtcag gtcggaaaca
     3301 gcataaatat ggattaaatt gctgcg
//
LOCUS       DAZFFG010000250         3291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFFG010000250 DAZFFG010000000
VERSION     DAZFFG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.252"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1213)
                     /locus_tag="ACHJ7J_004966"
     CDS             complement(<1..1213)
                     /locus_tag="ACHJ7J_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5003053.1"
                     /translation="MPMKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSL
                     AAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDDNTG
                     GQQIARGGTARNTTVTANGLQDVMAGGSATDTVISAGGGQNLRGQAYGTVLNGGEQWI
                     HAGGSASGTVINQSGYQTIKHGGQATGTIVNTGAEGGPESENVSSGQMVGGTAESTTI
                     NKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLDGGNQYVHKYGLALNTVINEGG
                     WQVIKEGGTTAHTTINQKGKLQVNAGGEASDVTQNTGGALVTSTAATVTGTNRLGAFS
                     VVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLAD
                     SGAAVSGTRSDG"
     gene            complement(1541..2413)
                     /locus_tag="ACHJ7J_004967"
     CDS             complement(1541..2413)
                     /locus_tag="ACHJ7J_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5003054.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            2833..>3291
                     /locus_tag="ACHJ7J_004968"
     CDS             2833..>3291
                     /locus_tag="ACHJ7J_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003055.1"
                     /translation="MTEFLEKMFDRVYSEKDFSINIAIFVSGIAGVTCYLILRDYVLT
                     LFSFVIIFPVVKIIAGGLYVRIITRKGEAVAEKRLATLYNSLTGREKEVVMHFVTHGG
                     SVMTWGQMNRLDDPEPGVESLARRGLLNTSVTMDGMRETFELDLTLFNYAY"
BASE COUNT          763 a          868 c          847 g          813 t
ORIGIN      
        1 ttccgtcgct ccgggtacca ctgacagcgg caccggaatc ggccagcagt acaccgccat
       61 ttcccatgga tacggtggtg gcggtaccac cgttgcggac atccagcgtt ccgccatcat
      121 ccacacgggt attagtggct gtgtgtccgc tcagcacatc cagacgtccg ccattttcca
      181 gtacgacatt atcagcctta cccgccacaa cggagaatgc tcccaggcgg tttgtgccgg
      241 tgacggttgc agctgtgctg gtaaccagtg cacctcccgt gttctgggtg acatcagacg
      301 cttccccgcc ggcattcacc tgcagctttc ctttctggtt gatggtggta tgcgcggtag
      361 ttcctccttc cttaatcacc tgccagcccc cctcgttaat taccgtgttc agcgcaagtc
      421 cgtatttgtg cacatactgg tttcctccat ccagccgggt gttatgtgcc tctccgtgta
      481 ccgtctggtc accgccggcg taaatgaggg tgtcccgtgc catccccgaa gaccagataa
      541 cctgccggcc atttttgttg atggtggtgg attcagccgt ccctccgacc atctgaccgc
      601 tggacacatt ttcagactcc ggaccacctt ctgcaccggt gttgacgatg gttccggttg
      661 cctgcccgcc atgtttaatg gtctggtagc cagactggtt aatcaccgtt ccggatgcac
      721 tgccgcctgc atgtatccat tgttcaccac cgttaaggac agtaccgtaa gcctgcccgc
      781 gcagattctg tcctccaccg gcactgataa ccgtatccgt ggcactgcca cctgccatga
      841 catcctgcag accgtttgcg gtgaccgtgg tgtttctggc agtgccaccc cgggctatct
      901 gctgaccgcc ggtgttgtca tcagagtccg gccccagctc aagcccggta ctgacagtca
      961 cgccatcagc tgttccggat acaaactggt tgtcatggtt taccagtgtt ccgccattca
     1021 ctgtttcacc cgggtgcaca acgatgtcag cagccagcac cgggagtgac gtgactgcgg
     1081 caagagacag tgcaaccgcc acaccgccac gtttaccccg tgcgcgggcc agttcggagg
     1141 caaccacgaa agcgcccgta atgtgattcc ataccagcct gtagcaggta ttcagatgtc
     1201 gtttcatcgg catttcctta atcagagtga gggtgaataa aaaagccggt atccacagat
     1261 gtggataccg gcaggtaaag ggttacagtg ccggaggtcc ggctgacgga agtttctgac
     1321 gtgtggctct gttgttccct aaatctcatg tctgtgtgta tctgtgcacg ttgctaacag
     1381 gagttattat tagcataacg atcgatatta acgatcagtt aaatagttat cgatcgtttt
     1441 attctattat ccggctattg cacggacaag agagacagta acgaaaacag cgcttttgtg
     1501 agcagactca gaggcaggga ggacatcatc cacaacaggt tcagaagaag atccagttcc
     1561 ataccgcgcg ggcaacagag accacggtgt cacgaacagc gcgcagggcc gtacggacaa
     1621 cagaggcaac acagacgctc tccgctgtgt caaatatccg gtccaccgca ccggtaaact
     1681 gttcacgggc ctgagagcgg acagactccg tgcgcagctc gtcctgcagt cgggtcatca
     1741 gggggctggc ggcatggtcg ggaagcgctg tcatgagcgc actgaccagc gtatccagtt
     1801 cccagccggt gcgggccgat acggcaacaa ccggatgtac gggccggaac agacggaata
     1861 ccgcctccgt tttctcacga atgttctgtt cctgtgcggg agaaggctga atacctgcca
     1921 tatcccattc atggcagggt tccgttttgt cagcctgcgt caccacaaac agcacctgct
     1981 gatgtccgca ctgcaggatg tgtcgccaga aatactcatc cacagacagg gcccggtcat
     2041 cggctttaat caaccacagc accaggtcca gttcaggcag aatgtcacgg tacagggctt
     2101 catactctgc atccctgtcc cggctctcgc ccaccccggg caggtcagtg ataaccatgc
     2161 tgtgaccgtg accactcaga cggaagcgct gcacttcccg ggtgccggcg tgaacatcac
     2221 tgaccggtgt gacctccccc tgaaacagtg cattacagag tgaagattta ccggccccgc
     2281 ttttacccat aatgccaatc acgggttcgt gactggtgag tttgcgcaga tgttccagga
     2341 tgtgacgtga aagcgagtaa ggcagggaag aaaggggttt ttcaattgcc tcaatggcat
     2401 cagacggatt catacatgta ttcccggtga aaaaagacaa aaccccgcgc accggaaagg
     2461 tggcggggga ttcacggaga taatatatgt ctgaaattat ttaggatgca ggtatatgca
     2521 gctgatttgt atcagctcat tagtacagta ctaaacctca tctgacagtc cactctgtgc
     2581 caagagcgga cgttagaagt ataaaagcat ctttacaagc gggttgttgg ggagagtttt
     2641 gttgcgttcg ccacaactct tgagaatgct gctatttatt atggtagtat tgccagctaa
     2701 aatttataat gattagcgac tcaatagcca gttcaagcca gattttgatg ggacactaat
     2761 gaaatcctcc cgcactattg cggtatattc atacccactc gagcatcttt agtgttaaca
     2821 aggaatgaaa tcgtgacaga atttcttgaa aaaatgtttg atcgtgttta ttcggagaaa
     2881 gacttcagca ttaacatcgc aatatttgtc tccggaatcg caggagttac ctgttatctt
     2941 attcttcgcg attatgtact gactctgttt tcttttgtta ttatttttcc ggttgtaaag
     3001 attattgcgg gaggattata tgtgagaatt attacccgca agggagaggc agttgcagaa
     3061 aaacgactgg caacgcttta taattctctg actggaagag aaaaagaagt agtcatgcac
     3121 tttgtgacgc atggtggttc tgttatgaca tggggacaaa tgaataggct tgatgatcct
     3181 gaaccgggtg tagaatctct ggcccgtcgt ggattactga atacgtccgt aacgatggat
     3241 ggtatgcgag aaacgtttga actagatctg accctcttta attatgcata t
//
LOCUS       DAZFFG010000251         3230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFFG010000251 DAZFFG010000000
VERSION     DAZFFG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.253"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            97..333
                     /gene="ycgZ"
                     /locus_tag="ACHJ7J_004969"
     CDS             97..333
                     /gene="ycgZ"
                     /locus_tag="ACHJ7J_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO5003056.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            376..648
                     /locus_tag="ACHJ7J_004970"
     CDS             376..648
                     /locus_tag="ACHJ7J_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO5003057.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSVQTLIKQLLVMKSAE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFNGKKINRIH"
     gene            677..943
                     /gene="ariR"
                     /locus_tag="ACHJ7J_004971"
                     /gene_synonym="ymgB"
     CDS             677..943
                     /gene="ariR"
                     /locus_tag="ACHJ7J_004971"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO5003058.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            1056..1304
                     /gene="ymgC"
                     /locus_tag="ACHJ7J_004972"
     CDS             1056..1304
                     /gene="ymgC"
                     /locus_tag="ACHJ7J_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO5003059.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            1640..3163
                     /locus_tag="ACHJ7J_004973"
     CDS             1640..3163
                     /locus_tag="ACHJ7J_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5003060.1"
                     /translation="MRNTLIPIIVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNSPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          946 a          628 c          698 g          958 t
ORIGIN      
        1 caaaaaatta atttatcatt ctgtacacat atttcgtaca agtttgctat tgttacctca
       61 cttaacattg attaacattt ttaacagagg cgtagcatgc atcaaaattc agtgacttta
      121 gattctgcgg gagcaatcac tcgttacttt gcaaaagcta acttgcctac tcagcaggaa
      181 actctcgggg aaattgtgac tgagattttg aaagatggac gtaatctgag tcgaaagtcg
      241 ctttgtgcca aacttctttg ccgactggaa caagcgacag gggaagagga acagaaacat
      301 tataatgcac taattgggtt gctttttgaa taatgcaaca gcctgattat ttaacaggtt
      361 agctatgaag tcgttatgaa gacatctgat aatgaaagta taaaatatga aattactggc
      421 caggcggtgc tccagatact gcgcatgaag ataaattttt cggtgcagac tcttattaag
      481 caattactcg taatgaaatc ggctgaggaa aatgctttcc gacgagattt aattgacagc
      541 ataattcgtg attttagcaa cagtgataca ggagggccaa accgaagaac agcgatagct
      601 gacaataaaa gtatgttcaa tggtaagaaa ataaacagaa tacattaaaa tttcataagt
      661 aagatgagag gttaccatgc ttgaagatac tacaattcat aatgcaataa ctgataaagc
      721 gttatcaagt tactttcgca gttcgggtaa tttgttagaa gaagagtcag cagtgttagg
      781 gcaggctgtg actaatttaa tgctttcagg cgataatgtt aataataaaa atattatctt
      841 aagtctgata cactccctgg aaacaacaag tgatattctc aaagctgatg tgatcagaaa
      901 aacactggaa atcgtgttgc gatacacagc ggatgacatg taacttttaa ttattaagta
      961 taagtttata gaaaactcat tcatcttatt tttgtctgtc gctttagact atacacagca
     1021 taattttatt gggttaatat ttctacgagg ctgacatgaa taattcaatc ccagagagat
     1081 ttatttttca atgtgctttg tttaaaaatc tcgagaggga ggtgttcatg acgcatgggt
     1141 atgttgatag tcatattatt gatcaggctt tacgcttgcg tttaaaagat gaaaccagtg
     1201 tgattctttc tgatctctat ctgcaaatct tgcagtacat tgaaatgcat aagactacac
     1261 taacggatat cattattaat gacagggaat ccgtgctctc ttagaacttg agtaaggtta
     1321 tagaataaac atcagctgta tcatcatgct ggtgcaaagt gagcatttca ggcgttatgc
     1381 tttcttaata tatccgcaat atcagggggc aaacaggaat ggagagcgct cgttttccat
     1441 tcttgacgcc tgatatccca cctaatttgt ttaattctgc ctacattgtg ctcaataatt
     1501 aataggttag atgaagatgg agaaaatttt atcttcggcg tggatatttg tgatgctctc
     1561 taccatgctc tctgtagacg ccagcgcgag cgaatgaacg tcattttcat gtattaaacc
     1621 ctttggccgg gacaagcaaa tgcgcaatac tctcataccc atcatcgttg cgatatgctt
     1681 gtttataacc ggtgtcgcta ttttgaacat ccagctctgg tattccgcaa aagcagagta
     1741 cctggcggga gcaagatatg ccgccaacaa tatcaatcat atacttgaag aagcgtcaca
     1801 ggcgactcaa acagcggtta acattgccgg gaaggaatgc gacctcgagg agcaatatca
     1861 gcttggcact gaagcagctc tgaaacctca cctgcgcaca atcatcattc tcaaacaggg
     1921 aacggtctgg tgtacatcct taccggggaa tcgggtcctg ctgtctcgta ttcctgtttt
     1981 cccggacagt aatttactgt tggctccggc aatcgacacc gttaatagat tacctatcct
     2041 gctctatcag agccaatttg cagatacgcg cattttggtt acgataagtg atcagcatat
     2101 tcgcggggcg cttaatgtac ccttgaaagg ggtaaggtat gtattacgcg tggcggatga
     2161 cattattgga cctacgggtg atgtgatgac gcttaatgga cattatccct ataccgagaa
     2221 ggttcactcc acaaaatatc atttcactat tatctttaac tcgccgccac tctttagctt
     2281 ctacagactt atcgataaag gctttgggat tttgatcttt attctgttaa tcgcctgcgc
     2341 cgctgccttc ctgctagata ggtatttcaa taaaagcgca acgcctgaag agatcctgcg
     2401 gcgggctata aataatgggg agatcgtccc tttttaccaa ccagtggtga atggtcggga
     2461 agggacattg cggggagttg aggtgttagc ccgctggaaa caacctcacg gtggatatat
     2521 ttcacccgcg gcatttattc cacttgctga aaaatccgga ttaatcgttc cgcttacgca
     2581 aagcctgatg aatcaggttg ccagacagat gaacgctatc gcgagtaaat tgccggaagg
     2641 ttttcatatt ggaattaatt ttagcgcctc gcatattatt tcgccgacgt ttgtcgacga
     2701 gtgcttaaat taccgtgaca gttttacccg ccgcgattta aacctcgttc tggaagttac
     2761 cgagcgtgag ccattaaatg ttgatgaaag tctggttcag cggttgaaca ttctgcatga
     2821 aaatggtttt gtcatcgcgc tggatgattt cggtactggc tactcagggc tttcttatct
     2881 gcatgacctg catattgatt atatcaaaat tgatcacagt ttcgttggcc gcgtcaacgc
     2941 agacccagaa tcaacccgaa ttctggattg tgtattggat ctggcgcgta aactttcgat
     3001 cagtatcgtc gctgaaggtg tcgaaacgaa agaacaactt gattatctga accaaaataa
     3061 tatcacattt cagcagggtt attatttcta taaacctgtt acatacatcg acttggtcaa
     3121 aattatcctt tctaaaccga aggtgaaggt tgtggttgag tgaaaatcga tcagtaaggc
     3181 catagtgcgg tgtaattata gacagctaat tagctcgttg cctcttgtta
//
LOCUS       DAZFFG010000252         3214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFFG010000252 DAZFFG010000000
VERSION     DAZFFG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.254"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            79..1221
                     /locus_tag="ACHJ7J_004974"
     CDS             79..1221
                     /locus_tag="ACHJ7J_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO5003061.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAQSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKTGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1695..1796
                     /gene="ykgR"
                     /locus_tag="ACHJ7J_004975"
     CDS             1695..1796
                     /gene="ykgR"
                     /locus_tag="ACHJ7J_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO5003062.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2160..2423
                     /gene="ykgM"
                     /locus_tag="ACHJ7J_004976"
     CDS             2160..2423
                     /gene="ykgM"
                     /locus_tag="ACHJ7J_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5003063.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            2423..2563
                     /gene="ykgO"
                     /locus_tag="ACHJ7J_004977"
     CDS             2423..2563
                     /gene="ykgO"
                     /locus_tag="ACHJ7J_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO5003064.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(2633..2824)
                     /locus_tag="ACHJ7J_004978"
     CDS             complement(2633..2824)
                     /locus_tag="ACHJ7J_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003065.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
BASE COUNT          960 a          703 c          658 g          893 t
ORIGIN      
        1 atcttgattc caaagctggc aacaactaac cagttaaaac atacgcatca aaatcagata
       61 aacatttgag gtattgttat gacaaataaa cacccatcat tgttttctcc ttttatgctg
      121 acagaaaaaa taaagctacg taatcgtatc gttatggctc ctatgacgac ctggtcagcg
      181 aatcctgatg gaaccatctc tgagcaagaa ctcgaatttt ataaacgccg ctcacaaaat
      241 gttggtttag taataacagg atgtacctat gtcacgccaa gcggcattgg gtttactcat
      301 gaatttgccg catatgacga ccgtttcatt aacagccttg aaaagttagc agcggcagcc
      361 caaagtgggg gtgctccggc tattttgcag attttccacg ccggtaataa agccatcccg
      421 gagcttgtac caaataacga tgttattagt gccagcgcat caagcgtaaa aactggtgac
      481 tttatgaaga gagtggtaca aagcagggaa atgactgaaa acgaaattca ggaaaccatt
      541 agagcattcg gagatgtcac aaaaagggcg attaaagcag gttttgatgg cattgaactt
      601 catggtgcgc acggattcct tctacagaat ttcttctccc cactatttaa ccagcgtaat
      661 gaccgatggg gaggagatct cgaaggacgt atgcgcttcc cgctggccgt attacaggaa
      721 gtaaaaaatg tagtctatga atatgcgaca aagccctttg ccatcggtta cagaatatct
      781 cctgaagaat ctgcaacagg ggggctgcgg atagaggata cttataaact ccttgatcgg
      841 ctcatttcat ccggtatttc gtatatacat acctcgctgg tcagtattaa cgatagctac
      901 cctgttgagt cgccaaatgg cccccgaact attgaattga ttttaaatca tattgcaggc
      961 agagtgccgg taatcgctgc aggtaaaata cgcacaccgt ctcaggctca agaagcaatt
     1021 tccactggcc tgccacttgt tgcgatcggt aaagggctgg tgataaatcc agaatgggtg
     1081 actttagctg aatcaggtag aggccatgaa attcaaacaa cccttaatcc acaacttgtt
     1141 cctgaattaa ccattccgga taaactatgg gatcaaattc aggcatcgaa aggaactggc
     1201 tggtttccgt taatggatta aatatctatt actctcccga gagtaaaact atcaaatcaa
     1261 tgagtaaccg atacagcctc acaaattctt tcagataatt aacccgtcac tgcggcacgt
     1321 tctctcataa ggcgtgcctg cagtgtaatc cagtatgtat ccggcatagt tgacggttta
     1381 atagagctca attcctcgcc caggttacca acaacgatat cttgtataac accccaggag
     1441 atacgacatt atgatgagat gcgatatgct cctcagcgcc aatcaacgcc aggactgggc
     1501 cagtcttgag atcagactca ttcccggaac tgacttcagg cgacatatat caatgacgtt
     1561 ggtgcaataa aagatagccg atgtaataca tctattcaaa tttgtgatac atgtcaaaaa
     1621 attggttgac caaactcgtt atttatataa gggcacttac gaagcgcgct cttttttaaa
     1681 gtgaggaagt accaatgaaa gagaataaag tacaacaaat cagtcataaa ctgattaata
     1741 tcgttgtttt tgtcgcaatt gtagaatacg cctatttatt tctccatttc tattaataac
     1801 ggcaataaac tgttcacttc agtgatattt aaaatatgca tcctctccct ttttgtaagt
     1861 aattattata tccgtgggag aggaatacac gctgtcaggt aatcaatcat actgcgataa
     1921 atcatcggcc agtaaagtgg agataacctc cattctcgaa aaatccatac tctcagcgaa
     1981 actatcatca atcactcatc caggcgttta tgggagcgtc gccaatggct gttaacaatg
     2041 ccagacttcc ccgttgggga aattccacat accaaaaatg ggcacaggga ttgggtgttg
     2101 aaatgatccg gatgagcatg tatctttaca gttatgttat aacataacag gtaaaaatga
     2161 tgaagcccaa tatccatcct gagtatcgta ctgtggtgtt ccacgacacc agtgttgatg
     2221 agtactttaa aatcggctcg actatcaaaa cagaccgtga gattgagctg gatggcgtaa
     2281 cgtatccgta cgtgacaatt gatgtctctt ctaaatcgca cccgttctat acagggaagc
     2341 tgagaacagt ggcatcagaa ggaaatgttg cacgattcac ccaacgtttt ggtcgttttg
     2401 ttagcacgaa aaagggggcg tgatgaaagt tcttaactct cttcgtaccg caaaagaacg
     2461 ccatccagac tgtcagattg tgaagcgaaa aggacggcta tatgtgattt gtaaatctaa
     2521 tccacgtttt aaggccgttc agggtcgtaa gaaaaaacgt tgattcaaaa ttcgacggat
     2581 taacgatatt tgtctgatta ataatcagac cggattaatg ttggtgtatt tattacacca
     2641 gcattaattt tcctggggat gtatcttcct gttcatttaa ggtcaactgc ctgacgtttc
     2701 tctccgaata ttccattata ttaatgttga cttgttgacc agcttcgccc ctgtatgctg
     2761 gcatcaactc tcttttagac tgaacacgcc actcagtctc ctccctttgc ggcgcagcct
     2821 gcattttcac tcaaactgtt aagatgataa atgtggtaaa tctgttggta ctaacataaa
     2881 aacgtttacg ccacaggaac agtctgatcc accggtaacc ccgtcgccga cgttcgagtg
     2941 ccagttagag taacgcgcac agataactgc actcagtgcc ctgacaaaga ggccatcgtt
     3001 cctgtgacag ctggcagcct tcgtttaact tcacttaatc tgactcttgg gggcttaccg
     3061 aacagatgac gtacatacgc ccgttcaatt ttccatcact tattgtaatg aacacctata
     3121 accattttgt gcggcatgtt aatccattaa aatttcttac tgattggcaa atcatcttca
     3181 atgacagctc attatagttt tatattctat ccct
//
LOCUS       DAZFFG010000253         3148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFFG010000253 DAZFFG010000000
VERSION     DAZFFG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.255"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..619)
                     /gene="dsbA"
                     /locus_tag="ACHJ7J_004979"
     CDS             complement(<1..619)
                     /gene="dsbA"
                     /locus_tag="ACHJ7J_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO5003066.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSE"
     gene            complement(636..1622)
                     /gene="srkA"
                     /locus_tag="ACHJ7J_004980"
     CDS             complement(636..1622)
                     /gene="srkA"
                     /locus_tag="ACHJ7J_004980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO5003067.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFNHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1699..1968)
                     /locus_tag="ACHJ7J_004981"
     CDS             complement(1699..1968)
                     /locus_tag="ACHJ7J_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO5003068.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2038..2622
                     /gene="mobA"
                     /locus_tag="ACHJ7J_004982"
     CDS             2038..2622
                     /gene="mobA"
                     /locus_tag="ACHJ7J_004982"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO5003069.1"
                     /translation="MNLITTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2604..3131
                     /gene="mobB"
                     /locus_tag="ACHJ7J_004983"
     CDS             2604..3131
                     /gene="mobB"
                     /locus_tag="ACHJ7J_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO5003070.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          821 a          767 c          763 g          797 t
ORIGIN      
        1 tctcggacag atatttcact gtatcagcat actgctgaac aaaaacatcc atattgctgg
       61 tatccatacc ctgcggattc agctgatatt taccgttaac aaacatcgcc ggaacaccgc
      121 gcaattgcac gtcagctgca gctttttcct gctgagcgac cagagatttc accacgaagc
      181 tgttccacgc cgcgtcgtac tcttcacctt taatacctgc gttgataaat acatcgcgga
      241 tatcagaagc agaacgaatg gtctgggttt tctgtacgcc ttcaaacagc ggaacagtca
      301 ctttgtcttc cacgcccagc gccatcgcca cagcccatgc ctgagtcaga tctttgccca
      361 ggtcaccacc catgaagttg acgtggtatt tagtcatctt cacgccttcc ggcagttttt
      421 tcttcacatt atcagaaata tgcagaactt cttcaaactg atagcagtgc gggcagaaaa
      481 aagagaaaaa ctccagcact tgcggcgcgc cagctaccgg tttttccagg gtagtgtact
      541 gtttaccatc ttcatactgc gccgccgatg cgctaaacgc taaaactaaa ccagccagcg
      601 ccagccaaat ctttttcatg atctactctc tccgattaat acataggtgt taattgcaaa
      661 gggggttctt gtagaacttt tgcctgttct ataaaagtcg ccgtctgtcg tagccagtaa
      721 tcttccccgg ttaaccacgg gaaatttttc gggaacgcgg gatcagccca acgccgcatt
      781 agccaggcaa gataataaac caaacgcatg gcgcgtaaag gttcaatcag tccgatttca
      841 gcggtgtcga actcgctaaa ttcttcataa gcttcaataa tagtttccag ttgcatccgc
      901 tgctcggctt tatcgccatt gagcaacatc cacaaatcct gaatagctgg accattacgt
      961 gcatcatcca gatcaacaaa cattgggcca tcgcgccaga gaatattccc ggcgtggcag
     1021 tctccatgta gccgcagaac ggtgaaatct tcccgccagt gtgctgtaac ggcggcgatc
     1081 agctcatctg tcgctttcag gaatgccgct ttcaacccgg aaggtatcag tgtagcgtcc
     1141 tcaaacagct tgcgtggctc aatgagatat tcattcagac cgatggtcgg gcgatggtta
     1201 aaaagctgtt tgcgccccgt ctggtgcata cgccctaaat aacgcccaac cgcttccatc
     1261 tgatcgatat tatcagcttc gaactggcga ccaccgacgc ttggaaaaac agcgaaataa
     1321 aatccctgat gattcaataa agtctgaccg ttaaaggcca caggtgctgc gaccgggact
     1381 tcatcattta ccagctgcaa cgcaaattga tgttcttcga ggatttgatc ggctgtccaa
     1441 cgttcagggc gataaaattt gacgacaaaa cgtcgacgat cttcgtcctg aaattgatag
     1501 acacggtttt catagctgtt aagcggggta agaccggaat ccacccggat cccttgctca
     1561 aacagagcgt ccatgatggt atccgggtgt agtgtctgga aagtaaaagc gctgttgttc
     1621 atcccatcat ccggaaaaca ttacgaatga tgaaggatat catttcacga tgattttggt
     1681 ggcgccgctt acaagctttt actctttaat tacgccacga gcgcgtaaca gcgccgtttt
     1741 gaagtcttct tcataatctt tctgcaaacc cgggatcacc gcatctttcg cggaatcacg
     1801 cattttcagg tgatagatca gaatgtcatc cgtcaaatcc gccagttcgc cgtcaaaacc
     1861 tgactctttc gccagtttct gcaaaaattg caacaggtta aggtctggct ctttttgcca
     1921 ggctggctgg aggagttcaa taacttcatt cagacgttta catttcatag taatgctcct
     1981 tactcttgag acagacacgt tagcagggtc aatcccacaa taaaagaggc gatatcggtg
     2041 aatctgatta cgacgataac aggcgttgtg ctggcaggcg gtaaagccag acgaatgggc
     2101 ggcgtagata aaggattgct tgaattaaac ggcaaaccat tatggcagca tgtcgctgac
     2161 gcgcttatga cgcagctctc tcacgtcgtg gttaatgcta atcgtcatca ggaaatctat
     2221 caggcaggcg gtctgaaagt gattgaagat tcactggcgg attacccagg ccctctggca
     2281 gggatgcttt cagtaatgca gcaggaagct ggtgagtggt ttttgttttg tccgtgcgat
     2341 acgccttaca ttccccatga tttagccgcc cggcttaatc atcagcgcaa agatgcgcct
     2401 gtcgtgtggg tccatgacgg tgaacgcgat cacccgacta ttgctctggt aaaccgcgct
     2461 attgagcctt tattactaga atatctgcaa gcaggagaac gccgggtaat ggcatttatg
     2521 cgtctggctg gcggtcatgc ggttgatttc agcgatcata aagatgcatt tgttaacgtg
     2581 aatacgccag aggagcttgc ccgatggcag gaaaaacgat gataccgtta ctcgcctttg
     2641 ccgcgtggag tggtaccgga aaaactacgc ttttgaaaaa actgatcccg gcattatgcg
     2701 ccagagggat ccgtccaggg ctgattaagc atacgcacca tgatatggat gttgataagc
     2761 caggcaaaga tagctatgag ctgcgcaagg ctggcgcggc acaaaccatc gttgccagcc
     2821 agcaacgatg ggccttgatg acggaaacac cagacgaaga agagctggat ctacagtttc
     2881 tcgcaagtcg aatggatacc tcaaagctgg atttgattct ggtcgaaggg tttaagcatg
     2941 aagagatcgc aaagattgtg ctgtttcgcg atggagccgg acatcgacct gaagaattag
     3001 tgatagacag gcatgttatt gctgtagcca gtgatgtgcc gcttaatctt gatgtcgcat
     3061 tactggatat taatgatgtt gaggggctgg ctgattttgt ggtggagtgg atgcaaaatc
     3121 aggacggata agcaagagta ggctggag
//
LOCUS       DAZFFG010000254         3053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFFG010000254 DAZFFG010000000
VERSION     DAZFFG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.256"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            167..1330
                     /locus_tag="ACHJ7J_004984"
                     /pseudo
     CDS             167..1330
                     /locus_tag="ACHJ7J_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1602..2695)
                     /locus_tag="ACHJ7J_004985"
                     /pseudo
     CDS             complement(1602..2695)
                     /locus_tag="ACHJ7J_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          802 a          703 c          702 g          846 t
ORIGIN      
        1 tcctacatat cactgaataa cagcaagagc tattttgagc ttctcctgtg ttcttaccgt
       61 atttttaaca tttctcttta tgttgtgacc actacttgga ttgatcttac ccagcaatag
      121 tggacacacg gctaagtgag taaactctca gtcagaggtg actcacatga caaaaacagt
      181 atcaaccagc aaaaaaacac gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa
      241 gcttgctgag cgcatcggtg ttgctgccgc agcccgtgaa ctcagcctgt atgaatcaca
      301 actctacaac tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga
      361 gatgtctact gagattgcac gtctcaaacg ccagctggca gaacgggatg gagagctggc
      421 tatcctccaa aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt
      481 gaaaaacatc aggctgagtt cagcatcaag gctatgtgcc gcgtgctccg ggtggcccgc
      541 agcggctggt atacgtggtg tcagcggcgg acaaggataa gccctcgtca gcagttccgt
      601 cagcgctgcg acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgca
      661 ccacgcctga cggatgaact gcgtgttcag ggttaccact ttaacgtaaa aaccgtggcg
      721 gaaagcctgc gccgtcaggg gctgagggca aaggcctccc ggaagttcag cccggtcagt
      781 taccgcgcac atggcctgcc tgtgtcagaa aatctgctgg agcaggattt ttacgccagt
      841 ggtccgaacc agaagtgggc gggagacatc acgtacttac gtacagatga aggctggctg
      901 tatctggcag tggtcattga cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca
      961 cgcatgacgg cgcaactggc ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagaga
     1021 ccccggaacg ttatcgttca tacggacccg cggaagcctg tactgttcag tagattatca
     1081 ggcgctgctg aagcggcata atctgcgtgg tagtatgagc gcaaaaggct gctgttacga
     1141 taatgcctgc gtggaaagct tctttcattc gctgaaagtg gaatgtctcc acggggaaca
     1201 ctttatcagc cgagaaataa tgcgggaaac agtgtttaat tatatcgaat gtgattacaa
     1261 tcgctggcga cggcacagtt ggtgtggtga tctaagtccg gaacaatttg aaaaccagaa
     1321 cctcgcttag agctgtgtcc atattacgag ggtaggatca atgatgatgt gatagagtga
     1381 ataaacagca gcataatctt tagtatattc taatggagaa ccgcattacc gagagcatat
     1441 ccaccaactt aattatattt ttaaaggcga gtcagaacat gtagtgatag tataatgctg
     1501 atgagtataa gttcttaagc gttattaatg cgacttaatt aaatatgagt cgaaaatttt
     1561 ctggtgtcct tgacccacga tatttagaca gtcgttgtat cttaagttga cgtaattcgc
     1621 tgacgtcgat cccttgatga gagataactt aacgcactat gcggatggtt ttcattgtaa
     1681 tgctcgaatc gccattgcaa aattatgcaa tgccgttctt acattcgctt tcgacatgaa
     1741 cgcaatgtag tcttcctcca tcgttttcac gaactgtttt gccattccat tactctgagg
     1801 gctacagatt gccatcgtac aggcccccag attcaactct ctggcgaact gccgtttttc
     1861 atgagccgta taagccgaac tattatcggt cagccactgg attgaccgtt ccggtccttt
     1921 atcgccaaag cgtttttcta tcgctcctaa catgacatcc tgcaccgttg atttgtcata
     1981 atctcccgtg cttactgccc agttatagct tcatgatcgc aacagtccag tgcgaaagtg
     2041 acacgcagtt tttcactatc atcacaaccg aactcaaagc catcggaata ccagcgctgg
     2101 gcgcttttct tcaccgatat tcggccttta tgttcccact gcggccggaa ggtttgtcat
     2161 gtaaccatat gtcggcatat cgctgatgat atcaaggatg cgtgctaacg cttccgcatc
     2221 cgcagtctaa tcacatctac aacgatgttt attcggctta ttaccagatg ttttcatcca
     2281 ttgtgccagt tgcgcacgag acacgcccag aatacggctt actagggtta ttccttgtcc
     2341 attggcaaca agggagcatg cgctatccat tttcgcgact gtccatatcc cccgcttctt
     2401 tgaggatctc aacttccatg gtttttttac tcagcaatag ctgtaactct tttatttgtt
     2461 atataacggc agccagtttt gaggcaggaa cgaaatcctc tccggcagca acagtagtga
     2521 gacttccttc catatattgt tttttccatt taaacaacaa gctgggctga atatcatgta
     2581 aacgtaccat atgggacacc gtcatgccag gctacattgt ctgttgaata atggcaattt
     2641 tttcctgagt agttttacgt atacgaagct cctgccccag caaagtttca gtcatctcaa
     2701 aattagaact agtgttagac atatatccaa gcctatctct tattttaaga tacatccact
     2761 gtctggcgtt ttaaggggac ggctcagcga aaaattttct ggcagaccag tacgtgaaga
     2821 tacagtaagg tctattctgg tttgttagcg taactagtag atcacacttt tgatatttac
     2881 agcgagtgag cagatctagg tgatatgact tgtatatgag gtatcaggag atgataaaat
     2941 aagaaaatga tgtaacagga atagacataa agcgtaacat taatgtcctt ttgacttttg
     3001 gttggatata attctgtcca cataactaat ttatttgtac ttattgagat tat
//
LOCUS       DAZFFG010000255         3011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFFG010000255 DAZFFG010000000
VERSION     DAZFFG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.257"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            113..1489
                     /gene="ygeH"
                     /locus_tag="ACHJ7J_004986"
     CDS             113..1489
                     /gene="ygeH"
                     /locus_tag="ACHJ7J_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO5003071.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYEFTPESIYR
                     AMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSNIT
                     NVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNMKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
     gene            1657..1875
                     /locus_tag="ACHJ7J_004987"
     CDS             1657..1875
                     /locus_tag="ACHJ7J_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003072.1"
                     /translation="MTNPIGINNFSQSSNIANVAGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMASFQNDMYSEDNPISPFYKIEW"
     gene            1959..2462
                     /locus_tag="ACHJ7J_004988"
     CDS             1959..2462
                     /locus_tag="ACHJ7J_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5003073.1"
                     /translation="MRSPKVKFLTIFTLSILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIRSLDYGLMQINQMHIPMLKKRGIIQDERDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMDNKKKRLQYAKKIYIVYTRLNELD
                     NRKALAK"
     gene            complement(2480..>3011)
                     /locus_tag="ACHJ7J_004989"
     CDS             complement(2480..>3011)
                     /locus_tag="ACHJ7J_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5003074.1"
                     /translation="GYKDLKKAIAECNKSAAQIFILGEFAGGMMGSELVKWVKSHKID
                     AHIITFVAKMPYIDSIKLLEAGAKGCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCE
                     KISSRYSSDNQLTNRESEILQLIADGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHS
                     GIELIKTALRMGVCTI"
BASE COUNT         1058 a          529 c          502 g          922 t
ORIGIN      
        1 gatatgtcga atgtaatttc ctgtagcgtt aatgacacat aaatatgaat agccataatt
       61 tctattgctt atgcaggatg caagaaacca attttttcat agaggttaac taatggactt
      121 agaaaataaa ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct
      181 tactcaagga aatgagcaag tttatattcc accgaaagag ttaggtgtat taatagtatt
      241 acttgaatcc gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa
      301 tattattgtt agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga
      361 aaaaattggc tatgatcgct gcatagaaac aatctaccgg aaaggttatc gtttcagcgg
      421 gcaggttttc aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt
      481 ccctttcacc acttcattga atacactgga tccattaata cttaatcagg aattagtaca
      541 aatcatttca aataaaaaaa tcgatggtct ctatacctat ccgatggctg cgacaaattt
      601 ttgtaatgac cacatatctc aaaattcatt cttgagcaga ttcaagccag attatttcgt
      661 tacaggaaga ataaaccaga ataatgcagt gaatacttta tacattgagt tgatcgacgc
      721 taaaaacctt ttcctcatcg ccagtaatca tctccctgtt gatgaactac ataatacatc
      781 acaatttatt atagataata tccttcaaac ggtacataaa ccagaacgat ctgtgagatt
      841 agctaagcag gaccaaggat ataagaatca ttatttatca gatgaaatgt tagccggaaa
      901 gaaagaactt tacgagttca cccctgaaag catttacagg gccatgacta tatttgatgg
      961 attacaaaat aaaagtgata tacagacgct aaaaacagaa tgttattgcc ttctagcgga
     1021 atgccatatg tctttggcac ttcatggaaa aagtgaactt gaacttgctg ctcaaaaagc
     1081 attagagctt ttagattatg tatcaaacat aaccaatgtc gatggaaaaa ttttagctat
     1141 tatgggactg ataactggcc tgtctggaca agcaaaagta tctcatatct tatttgaaca
     1201 ggctaagata cactcaactg atatagcctc tctctactac tatagggcac ttgtcaactt
     1261 tcataatgaa aaaattgaag aggcaaggat ttgtatagac aaatcactac aactcgaacc
     1321 cagaagacga aaagcagttg tgataaaaga atgtgtagat atgtatgtgc ctaacccgct
     1381 caaaaacaac atgaaactct actataaaga aactgagagt gaaagccatc gagttataat
     1441 tgacaacatt ttgaaactaa agcagctgac gagaatttgt atgcgataaa tttttattga
     1501 cgcaatttta ttgtttagat gtatgcaact tgttatttaa acaacagaga taaaaatatc
     1561 taagatattt accttattgc aagatattta aaatactcta gatttaaaag tatggtgtaa
     1621 acaccattgg ccaggacaat taaaaaaggg agctttatga ctaatccaat cggtataaat
     1681 aatttctctc aaagttcaaa tatagcgaat gtagcgggtg atgaagtcgt ttcattagat
     1741 aaacacatca acaccagtgc tactgataca gatcaaatac aagcatttat agtttcaaca
     1801 tggatggcgt cttttcaaaa tgatatgtat tcggaagata atcctatctc acctttctat
     1861 aaaattgaat ggtagacaat tgaccaaata aatcattagc gagatactat tataatagta
     1921 tctcgttcct ttcattcaat cctcacacat aaaaatatat gcgctcacca aaagttaaat
     1981 tcttaaccat cttcacgctt tctatattaa ttacaaaaat gtctttcgcc agcagcgctt
     2041 gctttaatga agcaggaaca atgttcagaa tcgaaccaaa tttgattaaa gctatcgctc
     2101 ttgttgaaag caacctaaaa aaggatagca taggcaagaa cagagataag aataacaata
     2161 taaggagttt agattatggg ttaatgcaga taaaccagat gcacattccc atgttaaaaa
     2221 aacgtggaat aatacaagat gaacgagatt tgttagataa cccctgtctg aacattaaaa
     2281 taggaaccga gattttatat aaccattttt cacgttgtgg agtaacgtgg caatgcctgg
     2341 ggacttataa tgcgggtttt gcaatggata acaaaaaaaa gagactgcaa tatgccaaaa
     2401 aaatatacat tgtatatacc aggcttaatg aactagacaa tcgtaaggct ttagcaaaat
     2461 gagcctaaag cctctttttt tatatagtgc aaacacccat acgtaaagct gttttgatca
     2521 actcgatacc actgtgaaca tctaatttct tcatgatatt gagtctatga gtttcgactg
     2581 ttttcctgct taactgcaag aaattagcga tttctttgtt tgttttacca tcagctatga
     2641 gttgtaatat ttctgactca cgatttgtaa gttgattatc agaagaatac ctggaagata
     2701 ttttttcaca atccatatgt acactatcaa aataagtgta gccattacta atcgaatcaa
     2761 tagcgcgatt tagtttcgcc gggtgactgg ttttccatac gcatcctttc gcacctgctt
     2821 caagcaattt tattgaatca atatacggca tctttgctac aaatgtaata atatgagcat
     2881 ctattttatg cgatttaacc cattttacga gttcggaccc catcattcca cctgcaaatt
     2941 ctcctaaaat aaatatttgg gctgcagatt tgttacactc cgctatagct ttcttcagat
     3001 ctttatagcc c
//
LOCUS       DAZFFG010000256         2863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFFG010000256 DAZFFG010000000
VERSION     DAZFFG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.258"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            159..386
                     /locus_tag="ACHJ7J_004990"
     CDS             159..386
                     /locus_tag="ACHJ7J_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5003075.1"
                     /translation="MWLPLTDEQRLVILKAYGIECDRLVREKERYEITSISRTQAWKL
                     EREGTFPPRKSIGKKSCGWLLSDLLCWIQTR"
     gene            492..716
                     /locus_tag="ACHJ7J_004991"
                     /pseudo
     CDS             492..716
                     /locus_tag="ACHJ7J_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
     gene            968..2401
                     /locus_tag="ACHJ7J_004992"
     CDS             968..2401
                     /locus_tag="ACHJ7J_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5003076.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     FSSACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLHKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDENLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNPALTVPVRLIPEELELFNWIKSYCIEKGIPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPIIEGKTLCVAPNFDL"
BASE COUNT          876 a          497 c          649 g          841 t
ORIGIN      
        1 ttcagtattt tgacccataa aaatcatagt attacatttt atttgcaagg tgttgactgg
       61 atgaatgata gtgactttca ctcattgttt atccgggaaa cttagtcatt tcatgctcat
      121 gccggaaata aaaagtattg atatatcagg agggtagtat gtggcttcca ttaacagatg
      181 aacagcgtct ggtcattttg aaagcttatg ggattgaatg cgacagactg gtacgtgaaa
      241 aggaacgata tgagatcacg tcaatttctc gtacacaagc gtggaaactt gaacgggagg
      301 gaacatttcc accacgtaag tctatcggaa agaaatcttg cggatggttg ttaagtgacc
      361 ttctctgctg gatacagacc agatgaactg tctgtatttt cagtatatcc agtctgtata
      421 gcagctattg ttgctattgt agcggaaata gctgctataa ccgaaagtta ttacgaaaaa
      481 aggaaatatt tatgtcttac atcaaaccgg atactccgta tccagtatat gcgcaaccat
      541 caatgactgg aaatgcaata attgaaactc aacacaatga aaaagcatct ctggctatga
      601 ctaccacatc attattaaca gcgatgtcaa ttgcctgtca gaatcagatt gacgtgtgca
      661 accccggaaa tttacgtggg cctgttaata tttacactat ggttctggct gattcctgag
      721 aaaggaaaaa gaccgggggg aaggtgttag tgaaataatt ttaggaggtg ttaagaatga
      781 gaaacatgaa tgatatttcg tggtaaataa cacagtatga actgaattaa ccttatcggt
      841 ttattaaaca tcagatctac agatattctg tatttctcag aacattaggt ctgaacagca
      901 gctatttcgc tattatagcg ggagttgctg ctactttact aacccattaa tgaaaagaga
      961 ggcttttatg tgcttacttg caccagaaaa tccttatccg atatatgcac taccaccgct
     1021 ggtgagaaat gcaataattg aaactcaaaa aaatacacag gcacctttgg ctatggtggc
     1081 gacatccgca ttaacagcgt tctcaagtgc ctgtcagaat cagattgacg tgtgcagacc
     1141 tggaaattta cgtgggcctg ttaatcttta ctctctgatt ctggctgatt ccggtgaaag
     1201 gaaaacgacc gtggataaag tgtttatgaa agcattttat ctcagggatg aagccctggc
     1261 ggaagaatac gcgaaactgg ttgagaatta cagtacagaa aaggaaatat gggagcaaaa
     1321 gcaaaaagca ctggagtcaa aattgcataa agaaattcgc gccggtaaag attgtaaggc
     1381 aacagagtca gagcttaaaa gacatctgaa taaatgtcct gttccgccgc agatacgccg
     1441 gacaattttc aatgagacaa cgatagaggg aatgttaaaa tattattccg acagcaatcg
     1501 ctcttttgct cttgtatcca gtgaaggagg aataattttt gatggccggg caatgagtaa
     1561 acttggaata cttaactctt tgtgggatgg tggttctctt ttcatcgaca ggaaatcatc
     1621 tcccggaatt attttgaagg atccaagact gacggtatcg gtgatgattc agcctgatgt
     1681 ttaccagaaa ggtttttgta cgcgaaaaaa agaacttgtg aaaacgtcag gacatcatgc
     1741 aaggtttttg atgtgtcaac caacatcaac gcaggggaca aggataataa ccggagataa
     1801 ttattcatca cagtatcagg atttgtttga acagaggatc aatgagctta ttgatgaaaa
     1861 tctggcgatg agtggtgaac gacgttgcct tcacttttct cctcaggctg ccagaatctg
     1921 gacggattat tacaacgatg tggaatcgaa gctgggggga ttggggcctt taagacattg
     1981 ccgggaatat gctgctaaaa atgcagagta tatggcaaga ctggcaggac ttattcacca
     2041 ttcgagcggt gaagaggggg acatttcccc ttacactgca gaaatggcga gagaattagc
     2101 aatatggtac ggtaatgagt atgtgcggtt gtctaatccg ttaacttttg acaaccctgc
     2161 cctgaccgta cctgtgcgac tcattccaga ggagcttgaa cttttcaact ggataaaaag
     2221 ctattgcatt gagaagggga tcccctgtat gaaaaaaaat gatattttac agcgtggtcc
     2281 gaaccgtttc cggaaaaagg ataaaatcaa ctggttactg gatttactgt atgaacaaaa
     2341 cagagttgta ccgattattg agggaaaaac gttgtgtgtc gcacctaact ttgacctctg
     2401 aacgacttaa tcgttgagta acatatagtc tcctctgcta cttgcggagg agactaatgc
     2461 ctttaccatg tcagcgttgt tgtattagaa aactctggta caggtcagtc agtaaatata
     2521 actggtgtaa ccactattta ctattggata gagtgttgct caggatattg tcggctttag
     2581 tctgaggtga ggtgggacct tcattgtttt cgtgttcaat aaataattta ggttaattag
     2641 tattattaat gggagttatg ttatctgtta tgttgtctta tctctttcta tcgtaatatc
     2701 actcctcttt atgctaagta ttctgtatgt gcgtaatgca tatatctcca tacactacag
     2761 tgtggtggtg tatggagttt agttatgtgt aaattatgtt actacactta tagcgtaata
     2821 ttgagaaagt taatccactg aatgtatgtc tggtttgtat tta
//
LOCUS       DAZFFG010000257         2858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFFG010000257 DAZFFG010000000
VERSION     DAZFFG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.259"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..190)
                     /locus_tag="ACHJ7J_004993"
     CDS             complement(<1..190)
                     /locus_tag="ACHJ7J_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO5003077.1"
                     /translation="MQHRQNNILTKMTLLSRALSVPCCDMFRRGSPWICYLSLSVFSG
                     FFIPAFSSPAAMLSPGDRS"
     gene            233..493
                     /locus_tag="ACHJ7J_004994"
     CDS             233..493
                     /locus_tag="ACHJ7J_004994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003078.1"
                     /translation="MSKNVSEKFSPEKVCRETVLWQNIRKYVRNVIGIVVLRKSIRQK
                     TAGNSLTFTEDKQHHFVIQQENRNPPSESGQGLYWQHNLKWH"
     gene            593..1824
                     /locus_tag="ACHJ7J_004995"
                     /pseudo
     CDS             593..1824
                     /locus_tag="ACHJ7J_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566974.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
     gene            1925..2494
                     /locus_tag="ACHJ7J_004996"
     CDS             1925..2494
                     /locus_tag="ACHJ7J_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003079.1"
                     /translation="MGKNNYPENKYRKWFSTAITYLITGGLCWYMLWASAISHYTVLQ
                     NTEPEWTTYFCTGITTGSLSFIYAFYIRTFNRTLKYLLNAFTGGFCLGFILSLNCYDV
                     CVYLFPDKVISYESEYDVVFPGPSIGKYGHCEAGLWLKDQNTSRWIQLCTNKEFLRSH
                     HKQGMTDVWVTARVNKIGSYIVKYEFIYM"
BASE COUNT          895 a          561 c          607 g          795 t
ORIGIN      
        1 cactgcggtc acccggagac aacatagctg ccggagacga aaatgcgggg atgaaaaaac
       61 cagaaaaaac ggagagggag agatagcata tccacggaga gccgcgccgg aacatatcac
      121 aacaggggac agacaacgca cgggataaaa gggtcatttt cgtcagaata ttgttctgcc
      181 ggtgctgcat gttctccccg tattctggcc tgtgtgatgc aagtgtaaac tcatgtcaaa
      241 aaatgtcagc gagaaatttt cacctgaaaa agtctgtcgt gaaactgttt tatggcaaaa
      301 catcaggaag tatgtcagaa atgtgatcgg tattgtggtt ttacggaaga gcataaggca
      361 aaaaacggcg ggaaattcat tgacattcac ggaggataaa cagcaccatt ttgttatcca
      421 gcaagaaaac cgtaatcctc cctctgaatc cggacaaggt ttgtactggc aacataacct
      481 gaaatggcac taatggccag agcttactaa tacataatcc ctacaggatg tagccaccag
      541 ttttgttcat ccggtattct gatttggtaa catgctccag aaaggaagaa atatgtactg
      601 caatgaatta aaagaatttg cgcaagagca ggaacaaaaa cttatcagtg cgcttgctga
      661 taccagagag cacccgtccc ccgatggtga aacaagaagc cgggataatt atcaacggga
      721 ttacgcgcgt attctgtatt catcatcatt tcgcaggctg cagggaaaga tgcaactttt
      781 tgaaattgat cctgaaaaat tcaacagaaa cagattaacg cacagtcttg aggttgctca
      841 gatcgcccga agtatcgcgt cagacctcaa actggttaac ccggtggtgg ttgaactggc
      901 agctctggcg catgacatcg gaaatcctcc ttcggtcatt ccggtgaaaa actgcttaat
      961 gaactgtctg aagaaatcgg cggctatgaa ggtaatgcac aggcgttacg tatcctgaga
     1021 aaactggaga agaaattttc atactgcaac ggattaaatc tgactcaccg tagtttactt
     1081 tcggttgtta agtatcccgc ccctcgcact gcaactactg ccggcaagtt catttatgat
     1141 gatgactttg atttttatac taacctgctt gctgaaaatc agctcgatct gaatcccgga
     1201 gagaaaacga ttgatgtgca gataatggat ctcgcggatg agattgccta cgccgcgcat
     1261 gacctggaag atgcccttag caggaacatg gtcacgattg aagatattga atatgagttt
     1321 cagatttctg acgaattccg gggagcgaag gaacagttca gggaaatcgt tactcagtcg
     1381 agaaacaccg cttttcaggc taacttacta aaaacctcag aagaatttgc catcatattc
     1441 cgcaaggagt taacttcaaa tattgtcaat cgcctcgttg cagatatttc tgtagtaacg
     1501 aacctgaatg gttttcagga gctggggttc gggaaactga atgcgttatc cgaagggctc
     1561 aaaaaacttc tctttaaagt catcatgcga aaacgtaata tcctcaccta tgagttcagg
     1621 ggaaataaaa taatcaggga tttatataac ttttacaatg aaggagagaa ttataaattt
     1681 ctgcctcctg aacttaaatt caccttaccc caaccagatt cctgtatatt tgaaatcagc
     1741 aaaaaacgag cagtggttga ctatatttca ggtatgatgg atacatttgc agtcaaggaa
     1801 tgggaaactc actgtctgaa gtaacactgt caattaccag aggcgcatgt tttcccatta
     1861 tggttatttc tcctttattc cgtttcggtg acaggttatt tttaaatatt ctgggaatga
     1921 gttggtggga aagaacaatt accctgaaaa taaatacaga aaatggtttt ccacagcaat
     1981 aacatatctt attacaggtg gtttgtgctg gtatatgttg tgggcatccg ctatatctca
     2041 ttatactgtt ttacaaaata cggaacctga atggacaaca tatttttgca ccggtataac
     2101 caccggttct ctttctttta tttatgcatt ttatatccgc acctttaaca gaacattaaa
     2161 gtatttatta aatgcattca cggggggatt ttgtcttgga tttatactga gtctgaactg
     2221 ttacgatgtc tgtgtttatc tttttccgga taaggttatt agctatgaat ctgaatatga
     2281 tgttgttttt ccgggaccat caataggaaa gtatggccat tgtgaagcgg gactctggtt
     2341 gaaagatcaa aacacttcca gatggataca actttgtaca aataaagagt tcctgcgcag
     2401 tcaccataag caggggatga cagatgtatg ggtaacagct cgtgttaata aaattgggtc
     2461 gtatatcgtg aaatatgaat ttatctatat gtaggtaaaa actgacatat ttaccatgga
     2521 tgagttgcta tatttttgct catacaggag taattgatta ctggctatac taaaaataca
     2581 gagaaaaact atcccaagat aaagattacc aactcacaga gacaagtctt gccttataaa
     2641 cagataaata tcatcaacac agattcagaa gctgaagcag taagccatgg ttaacagctt
     2701 gcttccagta cagataaaac aatctgtcac gctgactggc tcttatcact atcgttattt
     2761 catacctcta cagtataact catttatctt tattgccata tattagcatg aagtcaggca
     2821 ttttcatccc ctgccgataa tatatcaact ccggagaa
//
LOCUS       DAZFFG010000258         2842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFFG010000258 DAZFFG010000000
VERSION     DAZFFG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.260"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            110..2389
                     /gene="ydeP"
                     /locus_tag="ACHJ7J_004997"
     CDS             110..2389
                     /gene="ydeP"
                     /locus_tag="ACHJ7J_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO5003080.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFERCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLDSAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTQSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2637..2834
                     /gene="safA"
                     /locus_tag="ACHJ7J_004998"
     CDS             2637..2834
                     /gene="safA"
                     /locus_tag="ACHJ7J_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO5003081.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          760 a          659 c          693 g          730 t
ORIGIN      
        1 gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca
       61 tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa
      121 aattgaatcc taccagggtg ctgccggtgg ttggggtgct gttaaatccg tagcgaatgc
      181 agtacgtaag cagatggata tacgccagga tgttattgcc atgtttgaca tgaataagcc
      241 agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg cgtcattcga
      301 catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc aggtaaacgc
      361 ctctttcttt gctgagaata cggttcaatc attacttacc tggggagacc acgagcttga
      421 ggctgcgggg cgactcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc
      481 attaagctgg caacaagctt tcgatgaaat tggcgcacgc cttcaaagct atagtgatcc
      541 caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca
      601 gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga
      661 accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga
      721 agactttgag aggtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc
      781 tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa
      841 tcctctacag gaacgtggcc tggagcgatt tactgcaccg caaaacccgt ttgaaatgct
      901 gacgaactct gagactcagt tggacagtgc ctactataac gtgcgcattg gtggtgatat
      961 ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagcgccgc
     1021 aggtcggccc tcattgctgg atgacgaatt tattcaaacg cataccgtcg gctttgacga
     1081 gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt ctggattaag
     1141 tcagactcaa atcgccgaac tggctgatgc atatgccgct gccgaacgaa ccattatctg
     1201 ttacggaatg gggatcactc agcacgaaca tggtacccag aatgtacagc aactggtcaa
     1261 tctgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg
     1321 ccactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt
     1381 tctggctcgc ctgggtgagc gctatggctt cacaccacct caagcacctg gacatgctgc
     1441 aattgccagc atgcaagcaa tatgtacagg gcaggctcga gcattgatct gcatgggggg
     1501 caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     1561 tctggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     1621 ctatattctg ccggttctgg gacgtagcga gattgacatg caaaaaagcg gtgcgcaggc
     1681 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     1741 cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc
     1801 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     1861 tgaagctgtg ctgccagagt tcgccgatta taaccagcgc atccgacatc ctggtggttt
     1921 tcacctgata aacgctgctg ctgaaaggcg ctggatgact cagtcaggta aggctaattt
     1981 cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     2041 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     2101 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     2161 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     2221 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     2281 gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg
     2341 cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc
     2401 atttcttctg ctgtcatccg cacagcagaa gaatccctca ttgactatta tcgcgcaatt
     2461 tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt
     2521 aagaaactcc gttcagtact gaagcgccag tcctatttcc tcttttctcc ggcctgttat
     2581 attaagcata ctgattaacg atgcttaacg ttattcgctt aataaacata tttgaaatgc
     2641 atgcgaccac agtgaaaaac aaaatcacgc aaagagacaa ctataaagaa atcatgtctg
     2701 taattgtggt tgtcttatta ctgaccctta cgttgatagc cattttttcg gcaattgatc
     2761 agctgggcat ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata
     2821 gtttgcaaga ctgaaaataa ac
//
LOCUS       DAZFFG010000259         2759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFFG010000259 DAZFFG010000000
VERSION     DAZFFG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.261"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..524)
                     /locus_tag="ACHJ7J_004999"
     CDS             complement(<1..524)
                     /locus_tag="ACHJ7J_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5003082.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATRATDAADSARAASTSAG
                     QAASSAQEASSGAGT"
     gene            complement(585..>2759)
                     /locus_tag="ACHJ7J_005000"
     CDS             complement(585..>2759)
                     /locus_tag="ACHJ7J_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5003083.1"
                     /translation="TELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELL
                     ETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPS
                     SGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTLRQRLFRC
                     VSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTAD
                     SGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQLAPGNYR
                     LTVRAVNARGQQGEPASVSFRIAAPAEPSQIELTPGYFQITATPHLAVYDPTVQFEFW
                     FSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKSTFVEAVG
                     RASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLEEFSKEWKDASDKWNAMW
                     GVKIEQTKDGKHYVAGLGLSMEDTEEGKLSQFLVAANRIAFIDPTNGNETPMFVAQGN
                     QIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGTLNNVTVN
                     ENCTIKGMLEANQVRGDFVKTVSKSFPKKAGTWGNTETPNGTVTVTIFDDHNFDRQII
                     IPPIIFNGIAYYYPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDM
                     PSGGGNVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          551 a          859 c          677 g          672 t
ORIGIN      
        1 gttcctgcgc cggaagaagc ctcctgagcc gacgacgcgg cctgtccggc tgacgtgctg
       61 gctgcacgtg ctgaatctgc agcatcagtc gcacgggttg ccgcctcacg ggctgatgtg
      121 ctggcatcgc tggctgactt cttcgcggct gccgtgttct gtgccaccgc ggacgcgtta
      181 cgcgccacct cttccaccat cagttcaaaa cggcgcagag cctccggacg ggcatcatcc
      241 tccgtcatgg caccgagaaa atcattcagc gtaccgggtc gggaatcttc atacacggtg
      301 atggtcccgg catgtgacgg cgggaatcct tccaccaaca gaataacgct gtactgaccg
      361 tactcaacgt ccatgctgta acgcccggct tcatccggat tttctgaggc cagcgtgttc
      421 accaccaccg tggtgctgtt acgtctggct ttcagttgaa tagtgcagtt ctgtattggt
      481 tttcccgcac catctttcag cacacctgaa atctgtactg ccatactcac tccacaaata
      541 aaaaaggcgc catttctggc gcccgtattg gggttataaa tatttcaacg gatactgata
      601 ccggaagcgg cttttttggt cacaatcacc gtacagtctg tgatgttacc tgcgccctga
      661 ttgcctttct ggaaaatctt aaactccaga gtgacgttac caccaccact cggcatatca
      721 ataaccgcac tgtaactacc gggaatggct cctttagttt ctctggatgc gattaatacg
      781 ccatttttgc gaacttcaaa accataaccc gtgtatcgcg tacctcccgg gttatttccg
      841 ctccccggat agtaatacgc tataccgtta aaaataatgg gcggaataat gatttggcgg
      901 tcaaagttat gatcatcgaa aatggtgact gtaaccgtcc cgtttggtgt ttccgtgtta
      961 ccccacgtac cagccttttt cgggaatgat ttggatacag ttttaacgaa gtcacctctg
     1021 acctggttcg cctccagcat gcccttaatt gtacagttct catttaccgt gacgttgttg
     1081 agcgtcccgg agttcgcgtt cacgttaccg ctgatatccg catttttcgc cgtcagccgc
     1141 ccgtccggtg tcagggaaaa tgccggcgga ttgccgccgc tggtaatggt tggcgccgtc
     1201 agatacttca ggaacacttc gttcatgaat atctggttgc cctgcgccac aaacatcggc
     1261 gtttcattcc cgtttgtcgg gtcaataaac gcgatacggt tagcggcaac caggaactgg
     1321 ctcagtttgc cttcctccgt gtcctccatg ctgaggccaa gccccgcgac ataatgtttg
     1381 ccgtctttgg tctgctcaat tttgacgccc cacatggcat tccacttatc actggcgtcc
     1441 ttccactctt tcgaaaactc ctccagtctg ctggcgttat cctccgtcag gtcgactttt
     1501 tccagcaact ccttgccgag atgagattcg gttatcttgc ctttgaaaaa atccaggtaa
     1561 ccttccgcat catcgctcgc ccgaccgaca gcctccacga atgtcgattt gccgactgtg
     1621 ttcacactgc gaacgtaaaa ataataatca tggcccggct taatattgat actggcggct
     1681 atccagtaca gcgccgtacc aagataacgc gcgcttgttt caacctgcct gatatccgca
     1741 atccgctttt ccgagaacca gaactcaaac tgcaccgtcg ggtcataaac cgcaagatgc
     1801 ggcgtggcgg ttatctgaaa atagcccggt gtcagctcaa tctgtgacgg ctctgccggg
     1861 gcggcaatcc ggaacgatac cgacgccggc tccccctgct gcccccgggc atttactgcc
     1921 cggactgtca gcctgtagtt ccctggcgcc agttgcctga agcggtatgt ggtttccgtc
     1981 gtcctggccg tgctgaccag ccgctcactg ccgtcatccg ctgccacggt caggcgaagc
     2041 ataaagctca cccccttcac caccttcggc gtgtcccagc gcgccagcac ctgatattcc
     2101 ccgctgtctg cggtgacttc ggcagtcagg tgctgcaccg ctggcggcgt gacaccattc
     2161 accgtgccgc tctggtcacc gtcaaagtgc gccccgttat ccacgatggc ttctttttcc
     2221 ggtacatgct gcacggcggt gatggcatac gtgccgtcat cgttctcacg gatactcacg
     2281 cagcggaaca ggcgctggcg caacgtcggc aacttcagcc cccatacgct gtatccggca
     2341 acgccgtcag gaacacggct cacttttacc ttcacgccgt cggtgacgga ctggacctcc
     2401 acgctgaccg gattgccact tccgtcaacc aggcttatca gcgtggtgcc ggaggatggc
     2461 agcgtgattt cacggtcgag cgtcagcgtc cgggtctggc tgttcaccgc cagcacgcgc
     2521 ccgccggtgc tgatccccgc atagtcatca tcgcagattt caataacatc acccggtaca
     2581 tggcgaagcc cttcggcacc cacgctgaaa tccacggtct gcgtctccag cagctccgtt
     2641 ttaatcagcc acagcccggc gcggtgtgcc tgcccccggc tggtacagcc aaaggcatcc
     2701 atcttcgtaa cattacgacc gtaacgggca atggcctgcg tatcttcaac aagctctgt
//
LOCUS       DAZFFG010000260         2745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFFG010000260 DAZFFG010000000
VERSION     DAZFFG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.262"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>2745
                     /gene="rhsB"
                     /locus_tag="ACHJ7J_005001"
     CDS             <1..>2745
                     /gene="rhsB"
                     /locus_tag="ACHJ7J_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO5003084.1"
                     /translation="PLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLLLRDNTLI
                     LSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSP
                     HRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAG
                     EFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRD
                     NGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRG
                     RMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYCYEQNRITITDSLDRREVLHTQ
                     GEGGLKRVVKKEHADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPD
                     GRASAFYYNHHSQLTSATGPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLP
                     CATDDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYD
                     SRGQLIAVKDMQGHEMRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGGLTRS
                     MEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEG
                     LVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVTVHYGYDEKGRL
                     TGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLG
                     DTPLVEYTRDRLHRETLRSFGHYELTTAYTPAGQLQSQHLNSLQYDRDYTWNDNGELI
                     RISSPRHTRSYSYSNSGRLTGVHTTTSTLDIRIPYATDPAGNRLPDPELHPDSTLSMW
                     PDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQ
                     "
BASE COUNT          614 a          853 c          843 g          435 t
ORIGIN      
        1 cccgctgccg ttcatcctct cccgcaccta cagcagttac cggacaaaaa cgcccgcgcc
       61 ggtggggagc ctcggccccg gctggaaaat gcctgcggat atccgcttgc tgctgcgtga
      121 taacacactg atactcagtg ataacggcgg cagaagcctg tattttgagc acctgtttcc
      181 cggtgaggac ggttacagcc gcagcgagtc actgtggctg gtgcgcggcg gcgtggcgaa
      241 actggatgaa ggtcaccggc tggccgcact ctggcaggcg ctgccggaag agcttcgcct
      301 gagtccgcat cgttatctgg cgacaaacag tccgcagggg ccgtggtggc tgctcggctg
      361 gtgtgagcgg gtgccggaag cggatgaggt gctgcctgcg ccgctgccgc cgtaccgggt
      421 actgaccggg ctggtggacc gcttcgggcg cacacagacg ttccaccgcg aagccgccgg
      481 tgaattcagc ggcgaaatca ccggcgtgac ggatggtgcc gggcgtcact tccggctggt
      541 actgaccacg caggcgcagc gggcagaaga agcccggcag caggccattt ccggcgggac
      601 ggaaccgtcc gcttttcctg ataccctgcc gggttacacc gaatatggcc gggacaacgg
      661 catccgtctg tctgccgtgt ggctgacgca cgacccggaa tacccggaga atttacctgc
      721 cgcgccgctg gtgcgctatg gctggacgcc acgcggcgaa ctggcggcgg tgtatgaccg
      781 cagcaataca caggtgcgca gctttactta cgatgataaa taccggggcc ggatggtggc
      841 gcaccgtcac acgggccgac cggaaatccg ttaccgttac gacagcgacg ggcgggtgac
      901 agagcagctt aacccggcgg gcttaagcta cacctactgt tatgagcaaa accgcatcac
      961 catcaccgac agcctggacc gccgtgaagt gctgcacacg cagggcgaag gcgggctgaa
     1021 gcgggtggtg aaaaaggaac acgcggacgg cagcgtcacg cagagtcagt ttgatgcggt
     1081 gggcaggctt aaggcacaga cggatgccgc aggccggaca acagaataca gcccggatgt
     1141 ggtgacggga ctcatcacgc gcatcaccac accggatggc agggcatcgg cattttacta
     1201 taaccaccac agccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga
     1261 atatgatgaa tccggccgtc tgattcagga aaccgcccct gacggcgata tcacccgcta
     1321 ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gacgatgcca ccggcagccg
     1381 gaaaaccatg acgtggagcc gttacggcca gttgctgagc ttcaccgact gttccggtta
     1441 tgtaacccgt tatgaccatg accgtttcgg gcagatgacg gcggtgcacc gcgaggaagg
     1501 gctgagtcag taccgcgcat acgacagccg tggacagtta attgccgtga aagacatgca
     1561 gggccatgaa atgcggtatg aatacaacgc cgccggtgac ctgaccgccg tcattgctcc
     1621 ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccatcc gtaccacgca
     1681 gggcggtctg acgcgcagta tggaatacga tgctgccgga cgggtcatcc gcctgaccag
     1741 tgaaaacggc agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac
     1801 cggctttgac ggccgcacac aacgttatca ccacgacctg accggcaaac ttatccgcag
     1861 cgaggatgag gggctggtca cccactggca ctatgacgaa gcagaccgcc tcacgcaccg
     1921 caccgtgaag ggtgaaaccg cagagcggtg gcagtatgat gaacgcggct ggctgacaga
     1981 catcagccat atcagcgaag ggcaccgggt gacggtgcat tacgggtatg atgagaaagg
     2041 ccggctgacc ggtgagcgtc agacggtgca tcacccgcag acggaagcac tgctctggca
     2101 gcatgagacc agacacgctt acaacgcgca ggggctggcg aaccgctgta taccggacag
     2161 cctgcccgcc gtggaatggc tgacctacgg cagcggctgg ctggcaggca tgaaactcgg
     2221 cgacacaccg ctggtggagt acacccgcga ccgcctgcac cgggaaacgc tgcgcagctt
     2281 cggccattat gaactcacca ccgcttatac ccctgccggg cagttacaga gccagcacct
     2341 gaacagcctg cagtatgacc gcgattacac ctggaacgac aacggcgaac tcatccgcat
     2401 cagcagcccg cgccataccc ggagttacag ctacagcaac tccggcaggc tgaccggcgt
     2461 tcacaccacc acctcaactc tggatatccg catcccgtat gccacggacc cggcaggtaa
     2521 ccgcctgccc gacccggagc tgcacccgga cagcaccctc agcatgtggc cggataaccg
     2581 tatcgcccgt gacgcgcact atctttaccg gtatgaccgt cacggcagac tgacagagaa
     2641 aaccgacctc atcccggaag gggttatccg cacggatgat gagcgcaccc accggtacca
     2701 ttacgacagt cagcaccggc tggtgcacta cacgcggaca caata
//
LOCUS       DAZFFG010000261         2708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFFG010000261 DAZFFG010000000
VERSION     DAZFFG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.263"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>2708
                     /locus_tag="ACHJ7J_005002"
     CDS             <1..>2708
                     /locus_tag="ACHJ7J_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO5003085.1"
                     /translation="ASGGANTEIINTSGNIETHQGDIVVRTGHLLNQREGFSATTTTR
                     TNPSSIQGMGNALVDIPLSLLPDGSYGYFTREVENQHGTPCNGHGACNITMDTLYYYA
                     PFADSATQRFLSSQNITTVTGADNPAGRIASGRNLSAEAERLENRASFILANGDIALS
                     GRELSNQSWQTGTENEYLVYRYDPKMFYGSYATGSLDKLPLLSPEFENNTIRFSLDGR
                     EKDYTPGKTYYSVIQAGGDVKTRFTSSINNGTTTAHAGSVSPVVSAPVLNTLSQQTGG
                     DSLTQTALQQYEPVVVGSPQWHDELAGALKNIAGGSPLTGQTGISDDWPLPSGNNGYL
                     VPSTDPDSPYLITVNPKLDGLGQVDSHLFAGLYELLGAKPGQAPRETAPSYTDEKQFL
                     GSSYFLDRLGLKPEKDYRFLGDAVFDTRYVSNAVLSRTGSRYINGLGSDTEQMRYLMD
                     NAARQQKGLGLEFGVALTAEQIAQLDGSILWWESVTINGQTVMVPKLYLSPEDITLHN
                     GSVISGNNVQLAGGNITNSSGSINAQNDLSLDSTGYIDNLNAGLISAGGSLDLSAIGD
                     ISNISSVISGKTVQLESVSGNISNITRRQQWNAGSDSRYGGVHLSGTDTGPVATIKGT
                     DSLSLDAGKNIDITGATVSSGGDLGMSAGNDINIAATLISGSKSQSGFWHTDDNSSSS
                     TTSQGSSISAGGNLAMAAGHNLDVTASSVSAGHSALLSAGNDLSLNAVRESKNSRNGR
                     SESHESHAAVSTVTAGDNFLLVAGRDIASQAAGMAAENNVVIRGGRDVNLVAESAGAG
                     DSYTSKKKKEINETVRQQGTEIASGGDTTVNAGRDITAVASSVTATGNISVNAGRDVA
                     LTTATESDYHYLETKKKSGGFLSKKTTHTISEDSASREAGSLLSGNR"
BASE COUNT          692 a          698 c          762 g          556 t
ORIGIN      
        1 gcttccggcg gtgcaaacac agagattatc aatacctccg ggaatattga gacgcatcag
       61 ggcgatattg ttgtaagaac cgggcacctt ctgaaccagc gggagggatt ttctgccaca
      121 acaacaaccc ggactaaccc ctcatccatt cagggaatgg gaaatgctct ggttgatatt
      181 cccctttccc ttcttcctga cggcagctat ggctatttca cccgtgaagt tgaaaatcag
      241 cacggtacgc cctgcaacgg gcacggggca tgcaatatca caatggatac gctttattat
      301 tacgctccgt ttgctgacag tgccacacag cgctttctca gcagccagaa catcacaaca
      361 gtaactggtg ctgataatcc ggcaggccgc attgcgtcag ggcgtaatct ttctgctgag
      421 gctgaacgac tggaaaaccg ggcgtcattt atcctggcga atggggatat cgcactctcg
      481 ggcagagagt taagcaatca gagctggcag acggggacag agaatgaata tctggtatac
      541 cgctacgacc cgaaaatgtt ttacggtagc tatgccacag gctctctgga taaactgccc
      601 ctgctgtcac cggaatttga aaacaatacc atcagatttt cactggatgg ccgggaaaaa
      661 gattacacgc ccggtaagac gtattattcc gttattcagg cgggcgggga tgttaagacc
      721 cgttttacca gcagtatcaa taacggaaca accactgcac atgcaggtag tgtcagtccg
      781 gtggtctctg cacctgtact gaatacgtta agtcagcaga ccggcggaga cagtctgaca
      841 cagacagcgc tgcagcagta tgagccggtg gtggttggct ctccgcaatg gcacgatgaa
      901 ctggcaggtg ccctgaaaaa tattgccgga ggttcgccac tgaccggtca gaccggtatc
      961 agtgatgact ggccactgcc ttccggcaac aatggatacc tggttccgtc cacggacccg
     1021 gacagtccgt atctgattac ggtaaacccg aaactggatg gtctcggaca ggtggacagc
     1081 catttgtttg ccggactgta tgagcttctt ggagcgaagc cgggtcaggc gccacgtgaa
     1141 acggctccgt cgtataccga tgaaaaacag tttctgggct catcgtattt tcttgaccgc
     1201 ctcgggctga aaccggaaaa agattatcgt ttcctggggg atgcggtctt tgatacccgg
     1261 tatgtcagta acgcggtgct gagccggacg ggttcacgtt atatcaacgg actgggttca
     1321 gacacggaac agatgcggta tctgatggat aacgcggcca gacaacagaa aggactggga
     1381 ttagagtttg gtgtggcgct gacagctgaa cagattgctc agcttgacgg cagcatactg
     1441 tggtgggagt cagtcaccat caacggacag acagtcatgg tcccgaaact gtatctgtcg
     1501 ccggaagata tcaccctgca taacggcagc gttatcagcg ggaacaacgt gcagcttgcg
     1561 ggcggcaata tcaccaacag cagcggcagc atcaacgcac agaacgacct ttcgctcgac
     1621 agtaccggct atatcgacaa cctgaatgcg gggctgataa gcgcgggcgg tagcctggac
     1681 ctgagcgcca tcggggatat cagcaatatc agctcagtca tcagcggtaa aaccgtacaa
     1741 ctggaaagcg tgagtggcaa catcagcaat atcacccggc gtcagcaatg gaatgcgggc
     1801 agtgacagcc gatatggtgg tgtgcatctc agcggtacgg acaccggtcc ggttgcgacc
     1861 attaaaggca ctgattcact ttcactggat gcagggaaaa acattgatat taccggggca
     1921 acggtctcgt ccggtggaga ccttggaatg tctgcgggta atgatatcaa cattgccgca
     1981 accctgataa gcgggagcaa aagtcagtcc ggtttctggc acactgatga caacagttca
     2041 tcatccacca cctcacaggg cagcagcatc agcgccggcg gtaacctggc gatggctgca
     2101 ggccataatc tggatgtcac ggcatcctct gtttctgccg ggcacagcgc cctgctttct
     2161 gcaggtaacg acctgagtct gaatgcagtc agggaaagca aaaacagtcg caacggcagg
     2221 tcagaaagtc atgaaagcca cgcagctgtg tccacggtga cagcgggcga taacttcctc
     2281 cttgttgccg gtcgtgatat tgccagtcag gctgccggta tggctgcgga aaataacgtg
     2341 gtcatccggg gcggacgtga tgtgaacctg gtggcagagt ctgccggcgc aggcgacagc
     2401 tatacgtcga agaaaaagaa agagattaac gagacagtcc gtcagcaggg aacggaaatc
     2461 gccagcggtg gtgacaccac cgtcaacgca ggacgggata tcaccgctgt tgcgtcatcc
     2521 gttaccgcaa ccggcaatat cagcgtgaat gccggtcgtg atgtcgccct gaccacggcg
     2581 acagaaagtg actatcacta tctggaaacg aagaaaaaaa gcggaggttt tctcagtaag
     2641 aaaaccaccc acaccatcag tgaggacagt gcctcccgtg aagcaggctc cctgctgtcg
     2701 gggaaccg
//
LOCUS       DAZFFG010000262         2690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFFG010000262 DAZFFG010000000
VERSION     DAZFFG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.264"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..222
                     /locus_tag="ACHJ7J_005003"
     CDS             <1..222
                     /locus_tag="ACHJ7J_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5003086.1"
                     /translation="KIIEVAMLNHTTHPRGRDSHNLNKFTWRFIALSAAKPRVITIVA
                     TSEQEARQQSPAGCVMVFTARIRKEVRHA"
     gene            215..535
                     /locus_tag="ACHJ7J_005004"
     CDS             215..535
                     /locus_tag="ACHJ7J_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003087.1"
                     /translation="MPDMSNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDAKAMRIDMGNIGLLLAMLGHFDISLRCTIENATDALNAIKKANTD
                     SNRG"
     gene            542..841
                     /locus_tag="ACHJ7J_005005"
     CDS             542..841
                     /locus_tag="ACHJ7J_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5003088.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYRDKNGNRVTVKSIMFDRVYF
                     IRDGYSFHSSLNVEIFICRFRREIPPSRNNHVSRVDVDKKLQELKNMIAAWREQK"
     gene            838..2655
                     /locus_tag="ACHJ7J_005006"
     CDS             838..2655
                     /locus_tag="ACHJ7J_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO5003089.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERGELAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLHRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFANQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEESEDWMPSCVSVTNDDSKN"
BASE COUNT          744 a          561 c          752 g          633 t
ORIGIN      
        1 aagattatcg aggtagctat gttaaatcat accacacacc cgcgagggcg ggattcgcac
       61 aacctgaata aattcacctg gcgttttatc gccctgagcg ccgcaaaacc gcgtgtgatt
      121 accatcgtgg ccaccagtga acaggaagcc cgccagcaat ccccggctgg ctgcgtgatg
      181 gtgttcaccg cccgtatccg caaggaggtg cgccatgcct gatatgtcaa attaccagta
      241 cctgattaat ccgcatttta actgtgagca tgatattgct aaaaaggttt attccgctgc
      301 ggatggggct actgacaata tatcaatggc tgttgcgtca attggtagcc tgatgtggca
      361 tgcgtcagaa aatgaggaat atgacgcaaa ggccatgcgc attgatatgg gtaatatcgg
      421 tttgttactg gcaatgcttg gacattttga tatttcgtta cggtgcacca ttgaaaatgc
      481 cacagatgca ttaaatgcca taaagaaagc gaatactgat tcaaatcggg gataaataat
      541 catgagaacg tatttatctg gcttgactgc cagcggttat gcacatccca aaattatccc
      601 cggcgctatt tatcgggata agaacggcaa cagagtaaca gtaaaatcga taatgtttga
      661 ccgtgtgtac tttattcgtg atggctattc atttcatagc tcgctgaacg tggagatctt
      721 tatttgcaga ttccggcggg aaatcccgcc ttccagaaat aaccatgtgt cacgtgtgga
      781 tgtggataaa aaactacagg aactgaaaaa catgattgcc gcgtggagag agcagaaatg
      841 aaaaaagcgc caaatttaaa acaccaaccg cgtgacaaaa tgacggaagt catcattttt
      901 gcgggtagtg atgcgtgggc acatgcgaag cagtggcagg aacaggacgg gcgactggct
      961 ggcgataacg tgccacctgt ctggcttgga gagcaacaac ttgccgaact ggacaacctg
     1021 caaatcgtac cagacggacg ctatcgtgtg cgtctctatc aggcggggtt attgcgtccg
     1081 ggacttgtta ataccatcgg gcagaaactg gcagcggcag gtgtcaggga tgctgattat
     1141 taccctgaag gaatgcacag ccagaaacgg gagaactggc gcgaatatct ggagcgtgag
     1201 cggggagagc tggcggaaaa gaaaaaggtt gttgaactgc ctgtaaagaa aaaagagcgg
     1261 gtaaaagacg ataacgcttc atcactggcg cttaaccaga tgggagcaag tcaacgcggc
     1321 gaagttctcc tggcacatta tggtggtgaa ctggcgattc atgctgactc tgacactgtt
     1381 caccattaca acggcgttgt atgggagccg gttcaggata aagagttaca gcgtgctatg
     1441 gcgcagattt tcattgatgc ggagatcagc tattcacaga acgctattaa atcagcggtc
     1501 gataccatga agttaagttt gcctgtaatg gggaatacag cccgtaactt gattggattc
     1561 agtaacgggg tatttgatac cagaacaggt aattttcggg agcataacaa aaacgactgg
     1621 ttgttaattg ccagtgaatt acctttcagc ccaccagcag agggggaaac gctggcaaca
     1681 catgcgccga atttctggaa gtggttgcac cgttcggtgg ctgagaatga tcgcaaagcg
     1741 gatcgtgtac tggcggcatt attcatggtg ctggcgaaca gatatgactg gcagttattc
     1801 attgaggtaa ctggtcctgg tggaagcggt aaaagcgtaa tggcggagat ttgcaccatg
     1861 ctggcgggta aggctaatac agtatcagca agcatgaagg cgctggaaga tgcaagggaa
     1921 cgtgcgttag tggttggctt ttctctgatt atcatgccgg atatgacccg ctacgctggt
     1981 gatggggcag ggattaaggc cattacaggc ggtgacaagg tggcaattga cccgaaacac
     2041 aaagctccct actcaacgcg tattccggca gtagtgctgg cggttaacaa taacgccatg
     2101 tcattcagtg accgcagcgg ggggatctca cgtcgtcggg tgatattcaa tttttcggaa
     2161 gttgtaccgg agaacgaacg tgatccgatg ctggcggaaa aaatagaagg tgagctggcg
     2221 gtagtgattc gccatctgct tacacggttt gctaaccagg acgaagccag acgcctgtta
     2281 tatgagcagc agaaatctga agaagcactg gcgataaagc gagaggggga ttcgctggtg
     2341 gacttctgcg gctatctcat ggcgtcggta atgtgtgatg gcctgttagt gggtaatgct
     2401 gaaattgtgc cattcagccc gcgcagatat ctctatcatg cctatctggc ttatatgagg
     2461 gcacatgggt ttggtaaacc tgtaacactg acgcgcttcg gtaaagatat gccgggggca
     2521 atggcggaat atggcaggga gtatatgaaa cggaaaacga agcacggttt gcgttcaaac
     2581 gtgacactga cggaggaatc agaagactgg atgccatcat gtgtatcggt cactaatgac
     2641 gatagcaaaa attaaactta tggaataact gttcaccact gttcaccctg
//
LOCUS       DAZFFG010000263         2666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFFG010000263 DAZFFG010000000
VERSION     DAZFFG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.265"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            101..589
                     /gene="eco"
                     /locus_tag="ACHJ7J_005007"
     CDS             101..589
                     /gene="eco"
                     /locus_tag="ACHJ7J_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO5003090.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(965..2611)
                     /gene="mqo"
                     /locus_tag="ACHJ7J_005008"
     CDS             complement(965..2611)
                     /gene="mqo"
                     /locus_tag="ACHJ7J_005008"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO5003091.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          617 a          746 c          672 g          631 t
ORIGIN      
        1 ctttaagtta ttcataaagc aaattaataa atctgatgaa tatgttaacc ttcagcgaca
       61 tcatcggtgc aaacctataa atgaagaagg aaagcaaaaa atgaagacca ttctacctgc
      121 agtattgttt gccgctttcg ccaccacttc cgcctgggcg gcagagagcg tccagccact
      181 ggaaaaaatc gcgccttatc cacaagctga aaaaggcatg aagcgtcagg tgattcagtt
      241 aaccccgcaa gaagatgaat ctaccctgaa agtagaactg ttaatcggtc agacgctgga
      301 agtcgattgc aatttgcatc gtctcggcgg gaagctggaa agcaaaacgc tggaaggctg
      361 gggctatgat tattatgtct ttgataaagt tagctcgccg gtttccacga tgatggcctg
      421 cccggatggc aagaaagaga agaaatttgt caccgcgtat ctgggcgatg ctggaatgct
      481 gcgttacaac agcaagctgc cgatcgtggt gtatacgcca gacaatgtag atgtgaagta
      541 ccgcgtctgg aaggcggaag agaaaattga caacgcggta gttcgctaaa ctgctgtgaa
      601 gtgctccacc ccgtaggttg gataaggcgg tcacgccgca tccgacatct aacgcccgag
      661 ccggttgcct gatgcgacgc tgacgcgtct tatcaggcct acaccgctgt gaagtgcgtc
      721 accccgtagg tcggataagg cggtcacgcc gcatccgaca tctaacgccc gagccggttg
      781 cctgatgcga cgctgacgcg tgttatcagg cctacaccgc tgtgaagtgc tccaccccgt
      841 aggtcggata aggcggttac gccgcatccg acatccaacg tccgagccgg ttgcctgatg
      901 cgacgctggc gcgtcttatc agcatacgcc acatccggca taccatgccg gatgtggcac
      961 atcattacaa cgcaatatcc gccacttctt tttgaacggg ttgcggtttc aactgcggtt
     1021 tcggcgtact atctgctgct tgcggtttgt cgtatttcag ccccagcact tcgctggtgt
     1081 actgcaactc gcgttctgtt gcggctacat caccgttcag cttgcgtcca taagacggaa
     1141 cgatcgcttt caacgtagcc tgccattgcg ggctggaaac gcgatcgcca aatacttttt
     1201 ccagcagatc caacataatc ggcgcggcgg ttgacgcccc tggcgatgcc cccaggagcg
     1261 cggcaatggt tccttgctgg tcactgacca cttcagtacc cagacgcagt acaccacctt
     1321 tctcggcatc acgcttgata atctgcacac gctgccccgc ttgccacaaa cgccagtcct
     1381 cttttttcgc ttgcggatag tactctttca acgcttcaaa acgatcctct tcactcaaca
     1441 tcacctgact caccagatat ttcaccagat cgaaattatc cagcccgacg tgcatcatcg
     1501 gcatcacgtt agaggtggtg gtggaactca tcagatccca caatgaaccg tttttgagga
     1561 atttggttga gaaggtggca aatggcccaa acagcactac gcgtttaccg tccagaacgc
     1621 gggtatcgat atgcggaacc gacatcggtg gtgcgccaac ggatgcttta ccgtaaacct
     1681 tcgccagatg gtgattaacc acgtccgggt tttccgaaac aaggaactgt ccgcccaccg
     1741 ggaaaccggc gtagtctttc gcttccggaa tccccgattc ctgtaacagc ttcagcgccg
     1801 caccgcccgc gccgataaag acaaatttgg cacgaatgtt ctgtgcagtg ccatttttta
     1861 gatcggcaac ggtaacggtc caggtgttat cgtcattacg ctttagggcg cggacttcgc
     1921 tgctgagttg cagcgagaag ttagatttct tctgcaagga agcaattaac tggcgggtga
     1981 tctcgccgta gttcacatcg gtaccaattt ccgtacgcgt ggctgccact ttctgttgcg
     2041 gatcgcgccc ttccatcact aacggtgccc actctttgat ctgcgcgtga tcttcagagt
     2101 aacgcatacc gcgaaacagc gagctttgtt gcaacgcggc gtaacgggcg cgcaggaaat
     2161 taacgttatc ctcgccccag acaaagctca tatgcggaac ggtattgata aatgaacgcg
     2221 gagtacgcag cacgccgcgc tcgacctggt gcgcccagaa ctggcgggaa atctgaaatg
     2281 cttcgttaat ggcgactgct ttttcaatac tgatgctgcc atcggcgttt tgcggggtgt
     2341 agttcagttc catcagtgca gaatgcccgg ttccggcgtt attccagccg ttcgaactct
     2401 cctgcgcgac accctccagg cgctccacca tggtcatcga ccattcaggc tccagctcgc
     2461 gtaaataggt ccccaacgtg gcgctcataa tgccgccgcc aatcaacagt acatcagttt
     2521 cctgctcctc ggacgctttc gcttttgccg ccatcgaaac ggcattaagc cccacggcca
     2581 tcgagaagag catggcagtc acttttttca tcttgttaat gccttacttt tagtcgcttt
     2641 attgcaggtg agatttgcgc ggcatc
//
LOCUS       DAZFFG010000264         2587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFFG010000264 DAZFFG010000000
VERSION     DAZFFG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.266"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            86..1516
                     /locus_tag="ACHJ7J_005009"
     CDS             86..1516
                     /locus_tag="ACHJ7J_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5003092.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEMNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1557..2489)
                     /locus_tag="ACHJ7J_005010"
     CDS             complement(1557..2489)
                     /locus_tag="ACHJ7J_005010"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5003093.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHLQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPVADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          804 a          543 c          569 g          671 t
ORIGIN      
        1 attctgataa tgcatcctgc cgctggacat tatttcagca taagtggata ttcaaagttt
       61 tgctgtttta tcagggaata tttatatgat acgtaagtca gctacaggtg ttattgttgc
      121 gttagccgta atctggggtg gtggcacatg gtacacaggt acgcaaattc agcctggtgt
      181 cgaaaaattt attaaagatt ttaacgatgc taaaaagaaa ggtgaacatg cctacgatat
      241 gacgttaagt tatcaaaatt ttgacaaagg cttttttaat tcccgttttc aaatgcaaat
      301 gacattcgat aacggtgcac ccgatctcaa tatcaagcca ggccagaaag ttgtatttga
      361 tgtggatgtt gagcacggtc cgttgcccat cacaatgtta atgcatggta atgtcatccc
      421 agcactggca gcggcaaaag tgaacttagt gaataatgaa ctgacacaac cgctatttat
      481 cgccgcgaaa aataaatcgc ccgtggaagc gacattgcga ttcgcgtttg gtggctcatt
      541 ctccacgaca ttagatgttg cccctgcaga gtatggaaaa ttctcttttg gtgaaggcca
      601 gtttactttt aatggtgatg gtagttcatt gtctaacctg gatattgaag gcaaagtcga
      661 agatattgtt ctgcaatttt caccaatgaa caaagtaacg gcgaaaagtt ttaccattga
      721 ttctctgaca cgattagaag aaaagaaatt tccggttggt gaaagcgagt cgaaatttaa
      781 tcaggttaat attatcaatc agggggaaga cgttgcccaa atcgatgctt tcgttgcaaa
      841 aaccaggctg gatcgcgtta aagacaaaga ttatatcaat gtcaatctga cctacgaact
      901 tgataagtta acaaaaggga atcagcaact cggtagtggt gagtggtcat tgattgctga
      961 atctattgat ccctcagcag tgcgccaatt tatcatccag tataacattg cgatgcagaa
     1021 gcagcttgct gcacaccctg agttagcaaa cgatgaagtt gctctgcaag aaatgaatgc
     1081 tgcattgttc aaagagtatt taccgttatt acaaaaaagt gagccgacca ttaaacaacc
     1141 ggtaagatgg aagaacgcac tcggcgaact aaatgccaat ctggatatca gtattgccga
     1201 cccagccaaa tcttcatcat ccacaaacaa agatatcaaa tcgctcaatt ttgatgtgaa
     1261 gttaccgctt aatgtcgtca cagaaaccgc aaaacagctt aatttatctg aaggaatgga
     1321 tgcggaaaaa gcgcaaaagc aggctgataa acaaatcagc gggatgatga cattaggtca
     1381 gatgtttcag ttaatcacga ttgacaacaa taccgcctcg ctgcagctgc gttatacacc
     1441 gggtaaagtt gtttttaacg gacaggagat gagcgaagaa gaatttatgt ctcgtgccgg
     1501 acgttttgtt cattaaaata ctcactcgcc tctgctcatt cagaggcgag caaaatttac
     1561 ttgtcctgcc gctgtgatga catcaacgac gtcagaagcc ggtctttttc ctgccacagt
     1621 gagttcagcc attgctgaaa atggcgctta aagcttttgt cattgatgta gtcgccatgt
     1681 aactcatcgg caacaggctg caaatccaca tgtacgacaa tccgcgtcag tttaccgctt
     1741 aacatatcga agaacggctg acggttattg tccggataac acagcgtaac attaagcagt
     1801 ttatcgaatt gtttacccag tacatttagc gccatcgcaa tgcctgccgc ctttggtggc
     1861 aacaagtttt gaaaagtgga gtgggtttgc agatgttttt cttgcgtgaa acgggagcct
     1921 tcaacgaaat tgacaatggt ggtgggatgc aggcgaaact tttcacaaga acgccgagtg
     1981 gtttcaacat ctttaccacg tcgctccgga tgacgtaata aataagcgcg ggaataacgc
     2041 ttcataaacg gcatatccag cgcccagcac gccaggccaa gaaatggcac ccaggccagc
     2101 tgttgcttga ggaaatattt attcatcgga atgtgcttac gaaacagcac gcacagtacg
     2161 acaatatctg cccaactacg gtggttacaa ataagcagat accagttctt cttacttaac
     2221 ccttccagcc cgtgaacttc ccattgcagg tgtgggttca gatgcagtaa taccgccaga
     2281 ccttcacacc agcaatacat cataaaatca caaaaacgcg aaacctttcg ccaaatgact
     2341 ggcacaggta gcaatagttt tacaatcccg gcaatgatga tcgggacgga gcaaaaaata
     2401 gtgaccaaaa tggtcaatac gatactcagc aaaagggtta tcgcagcgag tattctcgtc
     2461 ataatgaatt tattcaaaag gttagccata gacagtgcgc taaagaagcg caaggcgcag
     2521 attttagcag aaaacgaccc aaataacgga tgatccttaa ggagaaaaat aattcatatc
     2581 tatccac
//
LOCUS       DAZFFG010000265         2568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFFG010000265 DAZFFG010000000
VERSION     DAZFFG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.267"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            21..386
                     /locus_tag="ACHJ7J_005011"
     CDS             21..386
                     /locus_tag="ACHJ7J_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003094.1"
                     /translation="MDKTFNYINKSKLSESLLRKHCVSVRLNNDELQLLNTKRGETSK
                     GEWLRLASLDKFPPIVPPINIEAWKSLSEISQKLNILISHLESKSDGSTLTKTEIFAV
                     KRQISELRSCLTASSTSVR"
     gene            392..1525
                     /locus_tag="ACHJ7J_005012"
     CDS             392..1525
                     /locus_tag="ACHJ7J_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO5003095.1"
                     /translation="MKGMQKIKRGKTFNGLISYLLKPASHHKSEPYVVGGNVIESFAE
                     ALSAEFNATKLLRSDVSKPVWHNSLRLPAGESLPIQQWKLLADDYMKRMDFSETHLRA
                     YVLHDDKDGQHIHIVASRIDATSGKLYLGKNENLISTRIIQDLEKDYQLTQTKGTDAL
                     NPSSSPVKRKKSRNETMLEERTGEKCAKTIIQEAIDTLITSKISEERFVQQLAAQKIK
                     AIPNRASTGKMNGFSFEYANISFKASQLGKKYSWKNLEPFILIDTIQTMESNATDFFW
                     AVNAEDILLPLPERPKPLLDEYLDTKTITATNSYINIAMRWIEAIPWLDNIVGIVKSL
                     NNPFFKHKNKPALIFSASPLSIDLTSVPSVEKSVKKNVFRPKI"
     gene            1765..2343
                     /locus_tag="ACHJ7J_005013"
     CDS             1765..2343
                     /locus_tag="ACHJ7J_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010616891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4755 domain-containing protein"
                     /protein_id="HFO5003096.1"
                     /translation="MKYFMMKRVLLAIALPVLVAYFGGSKVMSVYWVPLFFVSAMLLA
                     TAYGKYVVKKNNSSTFNYFNDNNDGIYLHKFNECEIRLDTNKKEVFLKNEKGAKTYSF
                     DDIKEWSYNITYGGEVAGAGLSSIRENGKTLGQNLSETGLFIRVKDVRKPEWHIRFYP
                     RNSAFNSHNGLNDLKAQLNQWMEIFDQNLNAA"
BASE COUNT          872 a          477 c          481 g          738 t
ORIGIN      
        1 aacatatgaa aggagttcgt atggataaaa cattcaatta tataaataaa agcaaattat
       61 cagagagttt gctaagaaaa cattgtgtta gcgtcaggct aaataatgat gaattgcagc
      121 ttctcaacac caaacgtggc gaaacaagca agggagaatg gcttcgatta gcttcccttg
      181 acaagtttcc accaatagtt cccccaatta atatagaagc atggaaatca ttgtctgaaa
      241 tctctcagaa gctgaacatc ctgataagtc atcttgaaag taaaagtgat ggaagcacat
      301 taacaaaaac ggagatcttt gcagttaagc gtcagatctc agagcttcgc tcatgcctta
      361 ccgcttcaag cacatctgtc aggtaggttg catgaaaggg atgcaaaaaa tcaaaagagg
      421 aaaaaccttt aatgggctaa tttcttattt attaaaacct gcttcgcacc acaagtcaga
      481 accgtatgta gtaggtggta atgtcattga gagcttcgca gaagcgttaa gtgcagagtt
      541 caatgcgacg aagcttctac gttcagatgt cagtaaacct gtttggcaca attctctgcg
      601 tctgcctgct ggtgagagtc tacctataca acaatggaag ctgttagccg acgactatat
      661 gaagcgtatg gacttctctg aaacgcatct acgtgcatac gtgcttcatg atgataaaga
      721 tggtcaacat atccatatag tagcaagccg cattgatgca acatccggga agctctactt
      781 aggtaaaaat gagaacctta tcagcacacg tatcatccag gatcttgaaa aagactacca
      841 gctaacacaa accaaaggaa ctgatgcact aaacccctct tcatcaccag taaaacgtaa
      901 aaaatcacgt aatgaaacaa tgttagaaga gagaacaggc gagaaatgtg caaagacgat
      961 catacaagaa gctatagata cgctcataac cagcaagatt tccgaagaaa ggtttgtgca
     1021 gcagcttgca gctcagaaga taaaagcaat tcccaacagg gcttcaaccg gaaaaatgaa
     1081 cggtttttcg tttgagtacg ccaacatttc attcaaggct tctcagcttg gtaaaaagta
     1141 ttcatggaaa aacttagagc cgttcatact cattgataca atccaaacga tggaatctaa
     1201 tgcaacagat tttttttggg cagtgaatgc ggaagacata ttgcttccat tacctgaacg
     1261 acccaaacct ctattagatg aatatttaga tacgaaaaca ataacagcta ccaatagtta
     1321 tataaatatc gctatgagat ggatagaagc aataccctgg ttagataaca tagtcggtat
     1381 cgtaaagagc ttgaataatc ctttctttaa acacaaaaac aaaccagcac tgatcttttc
     1441 tgcttcacca ctttctatag acctaacatc tgttccctct gttgaaaaat ctgtgaaaaa
     1501 aaacgttttc agaccaaaaa tttagcataa attaacctaa aaaactctat gctgctgatt
     1561 ttatgacaaa ataattgatc gttataatcg atcgattaat cacacttgat agtcatagac
     1621 tatcaagtta caagatatcg atcggtacaa atgatttgat aactttatcg cttcgtccaa
     1681 aatgtacctt gaacgaaatg tcggctaata gtttgattta actagcctat tttcaatatc
     1741 gttatatcat acaggaaaaa acgtatgaaa tacttcatga tgaagcgtgt gttgctggcg
     1801 attgcactcc ctgtccttgt tgcttacttt gggggttcta aagttatgtc agtgtattgg
     1861 gttcctctgt ttttcgtcag tgcaatgctt ctggctacag catatggcaa atatgttgtc
     1921 aaaaagaata actcatccac tttcaattat tttaatgaca ataatgatgg gatctatctt
     1981 cataaattca atgaatgtga aatccggtta gacacaaata aaaaagaggt tttcctcaaa
     2041 aatgagaaag gtgcaaaaac gtattctttt gatgatatca aagagtggag ctacaacatt
     2101 acatacggtg gtgaagtggc aggtgctggc ttaagtagta tcagagagaa cggtaaaaca
     2161 ttaggtcaaa acctctcaga aacaggttta tttattcgtg taaaagacgt tcgtaaacca
     2221 gagtggcata tcaggttcta tcctcgtaat agcgctttta attcgcataa tggcttgaat
     2281 gacctgaaag cgcagctcaa tcaatggatg gagatctttg atcagaactt gaatgcagca
     2341 tgaaattgag catcttatat ttaaaacaga atctatatag gaacagattc tgttttaagt
     2401 gtttttatta tagttcgcaa tatagttgaa atttctttct tttaatgacc ttgccaatac
     2461 ggaacgatca tattttcttt tttccgttat aaaaacagtt tctgtattag ctttgttctt
     2521 catgatgaag tgaatgatta acccgcacat ccttcttatt ttatcggc
//
LOCUS       DAZFFG010000266         2544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFFG010000266 DAZFFG010000000
VERSION     DAZFFG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.268"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..456
                     /locus_tag="ACHJ7J_005014"
     CDS             <1..456
                     /locus_tag="ACHJ7J_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO5003097.1"
                     /translation="DDTAVTGQEIWQEVQSGKWGEIATFSVTPELIAAAKDAKKREIE
                     AWRKEQESQPFTLEWSGRTWNAGPDSMARLYQAVMALKSDTARKTMVWGDAENQQVKL
                     SMQKLDELLTAMVQAQVDRNDEIYRRQREMKDELNNLDDLKSIRNFIVE"
     gene            complement(1429..1959)
                     /locus_tag="ACHJ7J_005015"
     CDS             complement(1429..1959)
                     /locus_tag="ACHJ7J_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5003098.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANVGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(2302..>2544)
                     /locus_tag="ACHJ7J_005016"
     CDS             complement(2302..>2544)
                     /locus_tag="ACHJ7J_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO5003099.1"
                     /translation="TYKSGKKGVRYLFECKDPESKAPKYIQFSDHIIAPRKSSHFHIF
                     MGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          736 a          490 c          584 g          734 t
ORIGIN      
        1 gatgatacgg cggtaacggg tcaggaaatc tggcaggagg tgcagagtgg taaatggggc
       61 gaaatagcca cgttcagcgt cacgcctgaa cttatcgcag cggcgaagga tgccaagaaa
      121 cgggaaatcg aggcgtggcg caaagaacag gaatcgcagc cgttcacgtt agaatggagc
      181 ggtcgtacct ggaatgctgg ccccgactca atggcccgtc tttatcaggc agtaatggca
      241 ttaaagtctg acacagcgcg aaaaaccatg gtatggggcg atgcggaaaa ccagcaggtg
      301 aaactgtcga tgcagaaact ggatgaactg cttactgcaa tggttcaggc gcaggtagat
      361 cgcaatgatg agatttatcg tcgccagcgg gaaatgaagg atgaactgaa taatctggat
      421 gatttgaagt cgatcaggaa ttttatagtg gaataacaga agccgcggca cgtcgtatgc
      481 aagaacgtac cgcggctggc tggataactt tctatagtgc gagtattgaa tgatttccag
      541 ccgttaccga ttttatataa ctgcagtgga attattcaac acacaggcaa aacggaataa
      601 gctgagaaaa aaaaagcgtg cttacccaat ctggggtaaa tcacgtctga atgacacctg
      661 aaaacaggtg gtggcctcaa gaaggcttgg aatattcttc tttaatgtta tgtaatttat
      721 tgatttttcg tgtacgattt taagacattt attccaagaa aaatttttaa ctctttgatt
      781 tttcgactct gtatcatcgg tctcgaaaac cggagtaggg gcaactctac cgggggttca
      841 aatccccctc tctccgccac tttatcaatg acttatctcc cgacttcccg ccttgctttt
      901 cctaaacaga acaatcgtag aatattcttg aagggttaga tcgtcactgt tttctgttcg
      961 atactgtgac attcagcact tgattcgcta tggatctgac aggaagcttt cgagcgaaaa
     1021 tgtgcagtta ttcagtcgtt ttcttatcgc tcaccattat tcttttagac attcatccta
     1081 tgaaactgcc gcaaagttgg tggtgggaac tgaagttgcg tagagaaggg gtcaataatt
     1141 gagtcaaaaa aggaacgcgg ccgtcagatt aaagccgcgg gacaaagtcc ataatttcca
     1201 agagttgttg aatttcccag cttttttgaa agcctttgta tcatactaac aagtaaaaaa
     1261 ggtttaaata ccaaaattaa cctgtatttt tgataagtct taccattgta cgatttacac
     1321 agtttctgct cctccttgtt gatagtggaa agggtgcatt gtgcacttgc gtaaacattt
     1381 atgacgcgtt gatgtccatg gttcacattt ccttgtcagg ggatcgactt atttgcggcg
     1441 aggcatcatt cgtaacagag tattatcttt ctgaatgtag tgatgaaaga gtgctgcgcc
     1501 agcgtgagct gcgattaaaa agtaacctac attcgcccag gtttcgtgaa tattttttat
     1561 cagtgcttta atttcactgt ttggggtaac aaagggagac acattgaaac caaggaaact
     1621 ccacgatttt ccactgtaag ccatcaaagc aatccccagc agaggaagtg caaggaaggt
     1681 tatataaagc atgatgtgca ttagcgaagc cgctttcatt tgccaggcag gtggcggtgg
     1741 tacaatagaa ggatcactat aacggtgttt tataatcagg cgtgaaaaca ttaacaccca
     1801 aacgaatata cccgcattgt aatgtgtttc tcgcatcagc agataagtac tactaccttt
     1861 aggaaaccag ccacggagtt ccatcgcggc ataggttatt gcaacgaaaa gcagcgttat
     1921 ccaatgtaaa taaatttgag tttttgagaa tcggttcatt tttgtagctc ctgtaatttt
     1981 cacagtcaag ctatcccacg aaccttaaaa attccttata cagatgtggt tttaagaatt
     2041 tgttatttaa aaaattgata gtgcacattg taaccagcca atataagcaa tggcgtgagc
     2101 acaggtatct ttctctgttg gccgtattgt gtatggaatc cattattggt taactcaatg
     2161 cgggttagct tattaatata cagacacgtc atagtgatga gcgtgtctgt agctagcttg
     2221 cgttcagtgg cgtttgtgga tggaccagtg gcagttatga tgacttacta aagcggcatc
     2281 gaggcgttat catgagaata ctcaatgaga catcatttcc tcgaccactt cttcgctact
     2341 caactgatat ggataatacg ttggccagtt ttccatttca tttaataaag attgctgtga
     2401 atcattaccc ataaaaatgt gaaaatgaga tgattttcgt ggtgcaatta tatgatcgct
     2461 aaattgtata tatttagggg ctttgctttc aggatcttta cattcgaata agtaacgaac
     2521 gcctttcttg cctgatttat aggt
//
LOCUS       DAZFFG010000267         2517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFFG010000267 DAZFFG010000000
VERSION     DAZFFG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.269"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            575..1562
                     /locus_tag="ACHJ7J_005017"
                     /pseudo
     CDS             575..1562
                     /locus_tag="ACHJ7J_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1968..2189)
                     /locus_tag="ACHJ7J_005018"
     CDS             complement(1968..2189)
                     /locus_tag="ACHJ7J_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003100.1"
                     /translation="MHFRKMKVVFLFIHVLAVVMISIMTVHLCSMAVGKVPEECDISV
                     RIAALVVLWLVFSGLIYWLFTAFMKTDVM"
     gene            complement(2204..2368)
                     /locus_tag="ACHJ7J_005019"
     CDS             complement(2204..2368)
                     /locus_tag="ACHJ7J_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003101.1"
                     /translation="MEDKKEDVVMQDMHFLLEDFICGHISRKTGRVPDRLRQRMDKLR
                     LQRVRKNRTD"
BASE COUNT          775 a          555 c          581 g          606 t
ORIGIN      
        1 ttatatttag actggccccc tgaatctcca gacaaccaat atcacttaaa taagtgatag
       61 tcttaatact attttttaga tagtcactgg agaatagatg attgatgcct tagggccgga
      121 gaaacgcaga cgccgtacta cacaagaaaa gatcgcgatt ggcggtcgtt ttgcatggtt
      181 tgcctccaga tattagtagc gcaaccattc ccatcgcagg acactcggga tgcagcataa
      241 agctcttatc aggtcacaaa ctcgggcccg gttatgttgt ggtgagaata tcacctttaa
      301 tcaggtggcc aaatttactg tgtcactata gacaaagaga tttaatttac atggtaagag
      361 catatggcac attacaacag gaggtctctg taaatatatc cagaaaacat atctcataaa
      421 acccggtaaa tgtaatgaac gcatccccaa ttaaatctgg agcacagtga tgaataaagg
      481 gggaaatcag agaggtgtgg agcctcgcag atttccggca tcatagtgcc caatacggga
      541 tgcataaagc tccttcagac tggagactcc acaaatgaaa tatacaccgg ttggtgttga
      601 tatcgcaaaa catctgattc aggttcactt catcgacgag cacacaggtg aagtgattga
      661 taatcaactg tgtcgtcagg attttctgat gttcttctgt aaccgggaac cctgcctgat
      721 tggtatggag gcttgtggag gctctcaaca ctgggcacgg gaactgacaa agaaggggca
      781 taaagtccgg ttgttgcagg cccgcttcgt taaagtgttc gtcatgggta ataaaaacga
      841 tgttatggat gcccgggcta tctggatggc agtacaacag cccggcaagg aaatcgctgt
      901 caaaacagaa gaacagcagt cggtactggt tctgcatcgt tcccgtagac aactggtgaa
      961 gttacgcacc atgcagatta actccctgca cggcacgctc ctggaatttg gtgaaacaat
     1021 tcacaaaggt cgggcagcaa tggagcggga gttccctgaa gcactggagc ggatgaaaga
     1081 gagactgcca ccgtatctca ttactgtgct ggaaaaccag tacaaccggc tgatttagct
     1141 ggactcactg atagaggata ttgaaaaaca gcttaccagc gtggcgaaac agaatgaaac
     1201 ctgtaagcgg ttgctggata ttcccggtgt tgggccactt attgcgacgg cagcggtggc
     1261 caccatgggg gaagcatcag cgtttaaatc agggcgagag ttcgcggctt atgttggtct
     1321 ggtgccaaga cagactggct caggagggaa aatccgcctg ccggggataa gcaaacgtgg
     1381 tgatacatat ctgcgaaccc tgtttatcca tggctggtcc gtggataacc gaactgaaaa
     1441 aacgtcgtcc ggtctgcgtg gcaatcgtcg ccatggcaaa caagctggca cgaacagtgt
     1501 gggcaatagc cgcccatgga cgtaaatacg ataaagaata cgtcagtatc aggtcatatt
     1561 aatcactgat accgataaac gatgaattct taataaaaag gcgaatgctg aaaggttgct
     1621 ctggcgacca gtgtgatgac aaagacaggt aagaccgtga ctcactaaac ctgaatgggg
     1681 ttttgggctt gaagcccgcg cagtaaatga ggtgtgagtc agcgaattac ataggggcta
     1741 gcagctttat ggctgcaata aagccggata taaaactgca acctaccgtc aatgccagaa
     1801 caatggaagc tgtacaaacg ggatgcgttc atataatgcc ccatgaatac aggatagtga
     1861 aaacagacat gataatcagg aaagccatac ctgttacaga agaaagtttc ctgcggggat
     1921 aatacataac cagaaacttt ctggccggtt atccatgtat attatattta cattacgtca
     1981 gttttcataa aggctgtaaa cagccagtaa atcagcccac tgaataccag ccacagcaca
     2041 accagagctg caatcctcac agagatatca cactcttccg gaacttttcc cacagccata
     2101 ctgcacagat ggactgtcat aatagaaatc atcaccacag caaggacatg aatgaaaaga
     2161 aaaacgactt tcattttccg gaaatgcata tacaacccct gactcagtca gtcctgtttt
     2221 tcctgaccct ctgcaatctt aacttatcca tgcgctgacg aagcctgtct ggaacacgtc
     2281 ctgtctttct tgaaatatga ccacagataa aatcttcaag cagaaaatgc atgtcctgca
     2341 ttaccacatc ttcttttttg tcttccatta aaacagcctg acaaaaatca aaatttacag
     2401 agagaaaata atacagtatc tgattgtact gatagcccat tggggacatc atgatggtac
     2461 caatcacttg taatatacaa actaccactt agctctacta tgataaaata tatcttt
//
LOCUS       DAZFFG010000268         2505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFFG010000268 DAZFFG010000000
VERSION     DAZFFG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.270"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            333..476
                     /locus_tag="ACHJ7J_005020"
     CDS             333..476
                     /locus_tag="ACHJ7J_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO5003102.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            489..698
                     /gene="cspE"
                     /locus_tag="ACHJ7J_005021"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             489..698
                     /gene="cspE"
                     /locus_tag="ACHJ7J_005021"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5003103.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNLTAI"
     gene            864..1673
                     /gene="rlmA"
                     /locus_tag="ACHJ7J_005022"
     CDS             864..1673
                     /gene="rlmA"
                     /locus_tag="ACHJ7J_005022"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO5003104.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1670..2236)
                     /gene="mntP"
                     /locus_tag="ACHJ7J_005023"
     CDS             complement(1670..2236)
                     /gene="mntP"
                     /locus_tag="ACHJ7J_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO5003105.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2233..2355)
                     /locus_tag="ACHJ7J_005024"
     CDS             complement(2233..2355)
                     /locus_tag="ACHJ7J_005024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003106.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT          670 a          595 c          604 g          636 t
ORIGIN      
        1 ctgttttatg agaaaaagtg atataacttt ttattcattg catagcagaa aatgtgatat
       61 tgcacgcact atgtaataac ttctcccact gacctggaac aactgaactt attgaactat
      121 gttagaaact acgtcagttt aagtatctgc cggaactgac aaggttaagc acaatgatat
      181 atcggcgtgt attccgttgc ataagtgtgc aaaaaaagtg gaagacgtat cgagatttgt
      241 gcgtctgatc gagacatgtt taaaaatggc ttgccataat taacgttgta tgtgataaca
      301 gatttcgggt taaacgaggt acagttctgt ttatgtgtgg cattttcagt aaagaagtcc
      361 tgagtaaaca cgttgacgtt gaataccgct tctctgccga gccttatatt ggtgcctcat
      421 gcagtaatgt gtcagtttta tctatgttat gcctgcgggc gaagaaaaca atctaaggaa
      481 tttttcaaat ggcaaagatt aaaggtcagg ttaagtggtt caacgagtct aaaggttttg
      541 gcttcattac tcctgctgat ggcagcaaag acgtgttcgt acacttctcc gctatccagg
      601 gtaatggctt caaaactctg gctgaaggtc agaacgttga gttcgaaatt caggacggcc
      661 agaaaggtcc ggcagctgtt aacttaacag ctatctgatc gaatccactg atctgaagtg
      721 tgaatacgct tcaatctcgc tataaagcct cgtcgaatgc gaggcttttt actatgcttt
      781 atcttcgctc ctggcgttcg gatatttgcc cgccgcgtga ttcgcgttac acttgcggcc
      841 tttagtatcc tgccggagtt gtcatgtcgt tttcctgtcc actttgccat cagcctcttt
      901 cgcgtgaaaa aaacagctat atctgtcccc aacgacatca gtttgatatg gcgaaagaag
      961 ggtatgtcaa tctgctgccc gttcagcata aacggtctcg tgatccgggc gacagcgcgg
     1021 aaatgatgca agcacgccgc gcattcttag atgccggaca ttatcagccg ctgcgtgatg
     1081 caattgtcgc ccaactgcgg gaacggctta atgaaaaggc cacggcggtg ctggatattg
     1141 gctgtggtga agggtattac acacacgcat ttgccgatgc gttgcccgaa atcaccacgt
     1201 ttggtctgga tgtttcgaag gtagcgataa aagcggcggc gaaacgctat ccgcaggtca
     1261 ctctttgtgt cgcttccagc caccgtttgc cgttttccga taccagtatg gacgccataa
     1321 tacgtattta cgcgccgtgt aaagcagaag aattagcacg agtagtgaag cccggcggct
     1381 gggtcattac tgccacgccg ggaccgcgac atttgatgga gctgaagggg ctgatttaca
     1441 atgaagtaca tcttcatgca cctcatgcag aacaactgga aggttttaca ttacagcaga
     1501 gtgatgagtt gtgttatccg atgcgtcttc gcggtgatga agccgtcgca ttattgcaga
     1561 tgacgccgtt tgcctggcgt gcgaagccag aagtctggca aacactggca gcaaaagaag
     1621 tgttcgactg ccagacggac tttaatattc acctctggca gcgttcttat taaccgtgga
     1681 agtgcgtcca gaggatctgg acgccgatgc cgatcagcac cagcccgccg agaatttccg
     1741 cttttttccc aataattgag ccgataaagc gaccaaccat catccctaat gtcgacataa
     1801 tcaaggttgc acaaccaatg gccaatgcgg tcgcgataat gttgacctga aggaaagcaa
     1861 gaccaacacc cacagccatg gcatccaggc tggtggcaat cgcggtggtt accagtagcc
     1921 agaaaccgtg ccgacggcgc ggctcttcat cttcatcatc tgcgccacga aaaccctcaa
     1981 taatcattcg cccgccgagg aatatcagca gcacaaacgc aatccagtga ttccattcaa
     2041 ggacaaaccg gctggctaac atgcccattc cccagccgat cagcggcgtc agggtttcga
     2101 cggcaccaaa aataaggccg gttcgcaatg cttcagaaaa tttcggttta tggagggtgg
     2161 cacctttacc gattgatgca gcaaatgcat ccatcgacat accaaacgca agaagaacag
     2221 tagcagtgat attcatgaca acgtcctgac cggggtatcc ataaaacaca tcatcgcccc
     2281 cagcaaacat gcatacatga acaacaagcc gcgatgggtt ttcgcgttca tgcaggcata
     2341 gcgattgatg tgcatatggt ctcgcctgac tgaaagtctt cagtccgtac gtgccacgtt
     2401 ttgcaacgag tatgttgaca cgtacatttc cggaatctgg aaattggcta ctccccaaat
     2461 gagggcgcaa ccttaacata ttttgagaat ataaaacaac tgttg
//
LOCUS       DAZFFG010000269         2462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFFG010000269 DAZFFG010000000
VERSION     DAZFFG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.271"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            22..2214
                     /gene="katG"
                     /locus_tag="ACHJ7J_005025"
     CDS             22..2214
                     /gene="katG"
                     /locus_tag="ACHJ7J_005025"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO5003107.1"
                     /translation="MKKVLSILIALALSNTSFANDDSSKKFYQPKTLDLSSLRMHSIE
                     SNPYGEQFNYTKNFSSLDIDAVKKDIHELLTNSQDWWPADYGNYGPFFIRMAWHGAGT
                     YRTYDGRGGADGGQQRFEPLNSWPDNVNLDKARRLLWPIKKKYGAKLSWGDLMVLTGN
                     VALESMGFKTLGFAGGRQDDWQSDLVYWGPGDKPLSDNRDKKGNLQKPLAATQMGLIY
                     VNPEGPNGNPDPVASAKDIREAFGRMAMDDEETVALIAGGHTFGKAHGAASPEKCVGP
                     APDGAPISEQGLGWKNKCGSGHGKDTISSGLEGAWSTAPTQFTMQYLKNLYKYDWVLD
                     KSPAGAWQWKPKNATNIVQDAHDPSQLHPLMMFTTDIALKTDPKYREITTRFLNNPDE
                     FKTAFARAWFKLTHRDMGPKARYIGSEVPSETFVWQDPLPKANYKTIDSKDISSLKKI
                     ITNSGLSNSELIKTAWASASTFRGTDYRGGNNGARIQLLPQKEWEVNEPKNLQLVLAA
                     LNNIKDNFNNNNKEGKKVSLSDLIVLAGNTAIENSAKKAGYNVSIPFTPGRTDATQSD
                     TDIISFNYLEPTADGFRNYYDKSRNRLSPVEALIDKASKLNLTVPEMTVLIGGLRVLN
                     INTNNSKNGVLTDTPGYLNNKYFINLLDMSTRWEESTKGSDIFKGYDRKTNQPKWNAT
                     SVDLIFGANPELRAVAEVYASDDAKDKFINDFIKVWTKIMNLDRFDMK"
     gene            2268..>2462
                     /locus_tag="ACHJ7J_005026"
     CDS             2268..>2462
                     /locus_tag="ACHJ7J_005026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003108.1"
                     /translation="MKKMLMLTGVMCLSIPFHTHAADLSEDMLILSQNLNIISKSESD
                     KEIITALDKMYNAAVDAMEEI"
BASE COUNT          834 a          412 c          519 g          697 t
ORIGIN      
        1 ttattaaaag agggggcgta tatgaaaaaa gtgttgtcga ttttaattgc tttagcatta
       61 agtaatacat catttgctaa tgatgattca tccaaaaaat tttatcagcc aaaaactctt
      121 gatttatcat cgttacgcat gcatagcatt gagtcaaatc catatggtga gcaatttaat
      181 tataccaaga attttagctc attagatatt gatgcagtca aaaaagatat tcatgaacta
      241 ttaacaaatt cacaggactg gtggcctgca gattatggta attatggacc attttttatc
      301 agaatggcat ggcatggggc cggtacctac cgtacatacg atggcagagg aggagctgat
      361 ggtggtcaac aacgctttga gccattgaat agctggccag ataatgtgaa tctggataag
      421 gctcgtagac ttttatggcc aattaagaaa aaatacggtg ctaagttatc gtggggagat
      481 cttatggttc ttaccggtaa tgtggcgctg gagtccatgg gatttaaaac attggggttt
      541 gccggtggaa ggcaagatga ctggcaatcc gatctcgtgt attggggacc aggtgataaa
      601 cccctgtcag acaatcgtga taaaaaaggt aatctgcaaa aaccattagc tgctacacaa
      661 atgggactta tttatgttaa tccagaaggc cctaacggta atccggatcc ggtagcatct
      721 gcaaaagaca taagggaagc atttggtcga atggcaatgg atgacgaaga aactgtcgct
      781 cttattgccg gtggacatac ttttggtaaa gctcatggtg ctgcatcacc tgaaaaatgt
      841 gttgggccag ctcctgatgg ggccccaatt agtgagcaag gtttaggctg gaaaaacaaa
      901 tgtggttcag gtcatggaaa agacacaatc agtagtggtt tggagggggc ctggtctaca
      961 gccccaaccc aattcacaat gcaatatctt aaaaacttat ataaatatga ttgggttttg
     1021 gataagagcc cggctggggc ttggcaatgg aaacctaaaa atgcaactaa tatagtccag
     1081 gatgctcatg atccttctca attacatcca ttaatgatgt tcacaacaga tatagcatta
     1141 aaaacagatc ctaaatatcg tgaaataaca acacgttttc tgaataatcc tgatgagttc
     1201 aaaactgcat ttgccagagc atggtttaaa ctgacccaca gagatatggg gccgaaagcc
     1261 agatatatag gtagcgaagt tccatcagaa acctttgtct ggcaagatcc tctacctaaa
     1321 gctaattata aaactattga tagtaaagat atttcctctc tgaagaaaat tataacaaac
     1381 tcaggtctga gtaattctga acttattaag actgcatggg cttctgcgtc aactttcagg
     1441 ggaactgatt accggggagg taataatggt gcaagaatcc aattgcttcc tcagaaagaa
     1501 tgggaggtta atgaacctaa aaatttacag ttagtgcttg cagcattaaa taatattaaa
     1561 gataatttca ataataataa caaagagggg aaaaaagtat cattatcaga tcttattgtt
     1621 cttgccggga atacagcgat tgaaaactcg gccaagaagg ctggatataa tgtcagtata
     1681 ccttttactc caggtagaac agatgcaact cagtctgata ctgatattat atcgttcaat
     1741 tatctggaac caacagctga tggattcaga aactactatg acaaatcaag aaacagactt
     1801 tctcctgttg aagccctgat tgataaagca agcaagctta atcttactgt tcctgagatg
     1861 actgtactca ttggaggatt gagagtatta aatattaaca caaataattc taaaaacggt
     1921 gtgcttactg acactccagg gtatcttaat aataaatatt ttataaatct cttagatatg
     1981 tcaacaagat gggaagagtc aaccaaaggt agtgacatat ttaaaggata tgatagaaaa
     2041 accaatcaac ctaaatggaa tgccacatcc gtagatctta tttttggcgc taacccagaa
     2101 ttacgtgccg tagctgaagt atatgcttca gatgatgcaa aagataaatt cattaatgat
     2161 tttattaaag tgtggacaaa gataatgaat cttgacagat ttgatatgaa ataattaact
     2221 ttcattgggg aggttcctcc ccaattctta ttataggagg ttattaaatg aagaaaatgc
     2281 tgatgttgac aggagtgatg tgtttaagta taccttttca tacacatgct gccgatttgt
     2341 cagaagatat gctaattctt tcgcagaatt taaatataat tagcaaatca gagagtgata
     2401 aagaaattat caccgctctg gacaaaatgt ataatgctgc tgttgatgca atggaagaaa
     2461 ta
//
LOCUS       DAZFFG010000270         2422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFFG010000270 DAZFFG010000000
VERSION     DAZFFG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.272"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            34..357
                     /locus_tag="ACHJ7J_005027"
     CDS             34..357
                     /locus_tag="ACHJ7J_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5003109.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNNDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            363..1499
                     /locus_tag="ACHJ7J_005028"
     CDS             363..1499
                     /locus_tag="ACHJ7J_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5003110.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1496..>2422)
                     /locus_tag="ACHJ7J_005029"
     CDS             complement(1496..>2422)
                     /locus_tag="ACHJ7J_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5003111.1"
                     /translation="IAASENISHAATVLGIAQANVSKYLADFESKVGLKVFDRTTRQL
                     MLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGIITYLSKHVIDKI
                     KDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYA
                     SQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDN
                     TQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYQPWR
                     VQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          595 a          563 c          618 g          646 t
ORIGIN      
        1 taattgttct tttttatatt ctctgaggga aatatgttcc tggactattt tgcactggga
       61 gtgcttattt ttgtatttct ggtgatattc tatgggatta ttattttaca tgatattccc
      121 tacctgattg ctaaaaaacg taatcatcct catgccgacg ccattcatgt tgctggttgg
      181 gtgagtcttt ttacgctgca tgttatctgg ccatttttgt ggatttgggc cacgctttac
      241 cgcccggagc ggggatgggg aatgcaaaac aatgactcct ccgttgtgca actacaacag
      301 cgtattgccg ggctggagaa acagctcgcc gacatcaaat cctcttctgc cgagtaatac
      361 ttatggattt actgattatt ttgacctatg tggctttcgc atgggcaatg tttaagatct
      421 tcaaaattcc tgtaaataaa tggaccattc ccacagcggc cctggggggc atatttattg
      481 tcagcggttt aattctgtta atgaactata accatccgta tacctttaaa gcgcaaaaag
      541 cggttatttc tattcctgtt gtcccacagg tgacaggcgt ggtaatagaa gtaacggata
      601 agaaaaatac gctgattaaa aaaggtgagg tgctatttcg actggacccg acgcgttatc
      661 aggcgcgggt ggatcggctg atggcggata tcgttaccgc agaacataaa cagcgggcgt
      721 tgggcgcaga gttagatgaa atggcggcga atactcagaa ggcaaaggcc acgcgggata
      781 aattcgctaa ggagtaccag cgttacgcac gcggcagcca ggcgaaagta aacccgtttt
      841 cagaacgcga tattgatgtg gcacggcaaa attatctggc gcaggaagcc tccgtaaagt
      901 catcggcggc ggaacaaaaa cagatccaga gccagctgga tagcctggtg ttgggtgaac
      961 attctcaaat cgccagcctg aaagcgcagc tcgcggaagc aaaatataac cttgagcaga
     1021 cgatagtgcg tgcgccgagc gatggttatg tgacccaagt gctgattcgt ccgggtacct
     1081 atgccgcgtc gctgccgcta cgtccggtga tggtgtttat acccgatcag aaacgacaaa
     1141 tcgtggcgca gttccgtcag aactccttgc tgcgcctggc tcccggcgat gatgcggaag
     1201 tggtatttaa cgctctgcca ggcaaagtgt tcagcggtaa gctggcagcc attagtccag
     1261 ctgttcccgg cggagcttat cagtcgaccg gcaccttaca gacgttaaac acagcgccgg
     1321 gttcagatgg cgttatcgcg accattgaac tggatgagca cactgatttg agcgctttac
     1381 cagacggtat ttacgcccag gtggcggtct actctgatca tttcagccat gtctcggtga
     1441 tgcgcaaagt gctgttacgc atgaccagtt gggtgcatta cctttatctc gatcattaat
     1501 cttcgctttc cgccgcttgt tcaggccgca gttgctgcga ctgagcaagc tggtggcgaa
     1561 tttcacgcag catttcatcc agcacatact ggacccgcca gggctggtat tcccgtttac
     1621 ggaagatagc caccagatcc agccaccatt catacgggtg ctgaaagcag ggcaccagcg
     1681 tgccgtcttg cagatcgctc tgcacacttt tgtctggcgc aaacacaatc cccaggtgat
     1741 ttcgtgccag ctccagcgcc gattgcgtgt tgtcgcaaac gtaatttccc ttcacgcgat
     1801 aatcccgcac ctctttactc ccggcgacgt tgaagcgcca gatattcgcg tcgtcgatca
     1861 tcatcgagtc gatcaaaata caggagtgat gttcgagttc gtcaggacgg ctaatgggat
     1921 gtttctcaag atagcgctgg ctggcataag cggttacggc atattgggta ataaaactgg
     1981 caaccagcga ttcatctttt ggtggggcat agctgattaa aacatcacaa tcatcaggaa
     2041 attcgacgcc ttcataaaag gcattacgct caagattgca ggtttttagc gataaggtga
     2101 tatcaccgat atctttaatt ttatcgatta catgtttgga taaataggta attatgccgg
     2161 ttggggcgta gatggtgacc cgaccacgtt tctcatgctt ataatctgca ataaaattat
     2221 taagctgctc atttctgtcc agcatgtcgt tgatgtacgg taatagcgcg gtgccaaaag
     2281 gtgtgagcat cagttgtcgg gtggttcggt caaaaacttt taaacccact tttgattcaa
     2341 aatcagcaag atatttgctg acgttggcct gtgcgatgcc aagtacagtc gcggcatggc
     2401 tgatattttc actggcagcg at
//
LOCUS       DAZFFG010000271         2389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFFG010000271 DAZFFG010000000
VERSION     DAZFFG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.273"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            131..634
                     /locus_tag="ACHJ7J_005030"
     CDS             131..634
                     /locus_tag="ACHJ7J_005030"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5003112.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(680..1096)
                     /gene="rraB"
                     /locus_tag="ACHJ7J_005031"
     CDS             complement(680..1096)
                     /gene="rraB"
                     /locus_tag="ACHJ7J_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO5003113.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1258..2262
                     /gene="argF"
                     /locus_tag="ACHJ7J_005032"
     CDS             1258..2262
                     /gene="argF"
                     /locus_tag="ACHJ7J_005032"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5003114.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKANKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSN
                     "
BASE COUNT          614 a          556 c          593 g          626 t
ORIGIN      
        1 tgccacgtta caatactctt atcagtaata ggctggcaaa aactttattg ctttataaga
       61 tgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt ataattactt tatttttatt
      121 gggttaaact atgaataaca ttgcgccgca atcacctgta atgcgtcgcc tgacgctgca
      181 ggataatcct gctattgccc gcgtcattcg tcaggtatcc gccgaatacg gtcttaccgc
      241 tgataaaggc tacaccgtcg ccgatccgaa tcttgacgag ctgtatcaag tgtatagtca
      301 gccgggtcat gcatattggg tggttgagta cgagggtgaa gtggtcggcg gcggtgggat
      361 tgcgccatta gcaggtagtg aatcggacat ttgcgaactg caaaagatgt attttcttcc
      421 ggctattcgc ggtaaagggc tggcgaaaaa actggcctta atggcgatgg agcaggcaag
      481 agaaatgggc ttcaaacgct gctatctgga aacgaccgct tttttgaaag aagcgattgg
      541 tttgtatgaa catttaggtt ttcaacatat cgactatgca cttggctgca cgggccatgt
      601 tgattgtgaa gtgcggatgt tgcgcgaact ctaaaaaaat gcccactagt gtgtgctatc
      661 gggcatttta atcaattaat tagtggcgaa ccccgtcatc gtcttcatcg acaaaatctt
      721 catcgtcgcc gtcttcgccg ttgggatctt caaagtaagt gccccaaccg tcgtactcaa
      781 catcaaattt ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc agatcggcat
      841 tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat atcaccatct tcaacttcca
      901 gctcttctgg atcggtcact tcgtaaccga gtttaaacgc ttcaactgct gctttttcca
      961 gggtttccag atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg tccggatcgc
     1021 tgccatcttc cagtaattct tcaataatca aacgtgtttc ttcacgctgt tcttccagtt
     1081 gttccgggtt tgccatggct cattcctctt aatgtcctgc cgatactcat attgtcacac
     1141 accactggca ttgcctccac ctttgtgaca aagatttatg ctttagactt gcaaatgaat
     1201 aatcatccat ataaattgaa ttttaattca ttgaggcgtt agccacagga gggatctatg
     1261 tccgggtttt atcataagca tttcctgaaa ttactcgatt tcacgccagc tgaactcaac
     1321 agcctgctgc agttagccgc gaagctgaaa gccaataaga aaagcggtaa agaagaagcc
     1381 aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag actcgacccg tacccgatgc
     1441 tctttcgaag ttgccgcata tgaccagggt gctcgcgtta cttatctcgg cccaagcggc
     1501 agccagattg gtcataaaga gtcgattaaa gacactgccc gcgtgctggg tcgcatgtat
     1561 gacggtattc agtatcgcgg ctatggtcag gagattgtcg aaacactggc ggaatacgct
     1621 ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc ccacgcagtt gctggcggat
     1681 ctcctcacca tgcaggagca tttacccggt aaagcattca acgaaatgac gctggtctat
     1741 gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg aagctgcggc gcttaccggt
     1801 ctggatttgc gtctggtcgc gccacaggca tgctggccgg aagctgcgct ggttacggaa
     1861 tgccgcgccc tggcacagca aaatggcggg aatattacgc tgacggaaga tgtagccaaa
     1921 ggagttgaag gtgctgactt tatctatacc gatgtgtggg tgtcgatggg ggaagcaaaa
     1981 gagaaatggg cagaacggat tgcattactg cgtgattatc aggtgaacag caagatgatg
     2041 cagttgaccg gtaacccgga ggtcaaattc ctccactgcc tgcccgcgtt tcatgacgac
     2101 cagacgacgc ttggtaagaa aatggcggaa gagtttggcc tacatggcgg aatggaagtg
     2161 actgatgagg tcttcgaatc tgccgccagc attgtttttg atcaggcgga aaaccgtatg
     2221 cataccatca aagcagtgat ggtcgcgacg ctcagtaact aattgatatc gctataaaga
     2281 tgccgggaaa tacaaaacta tcccggcatc acattatttt atgcagtgat aggtctattc
     2341 ttccacccaa ttttttctat tgcagaacgt aaatcttgcg gtggattta
//
LOCUS       DAZFFG010000272         2351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFFG010000272 DAZFFG010000000
VERSION     DAZFFG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.274"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(37..111)
                     /locus_tag="ACHJ7J_005033"
     tRNA            complement(37..111)
                     /locus_tag="ACHJ7J_005033"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(76..78),aa:Arg,seq:cct)
     gene            complement(187..1119)
                     /locus_tag="ACHJ7J_005034"
     CDS             complement(187..1119)
                     /locus_tag="ACHJ7J_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO5003115.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1413..2168
                     /gene="mlaA"
                     /locus_tag="ACHJ7J_005035"
     CDS             1413..2168
                     /gene="mlaA"
                     /locus_tag="ACHJ7J_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO5003116.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAKAIQDDLKDIDSE"
BASE COUNT          586 a          560 c          570 g          635 t
ORIGIN      
        1 tttatagaca atggtagacg ttggtgtacg gcgggatggt gtcccctgca ggaatcgaac
       61 ctgcaattag cccttaggag gggctcgtta tatccattta actaagagga caatgcggca
      121 tgagtatacc cgctaatgga ctgcggggta agtacgctgc cgtgcgattg cttaaaccct
      181 cgccatttat gccgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg
      241 gcgtgcttct gctttgcgct tgttgctcat gtcgttacgg atctgtgcat gactcattaa
      301 cgcgaagata aaggtgccgc cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca
      361 gatgaaatcg ctccagtgca gcgtaccgtt aaacaccaga tagaggattt caacagaacc
      421 gacaacgatg tgagtggtgt cacccagggc aataagccag gtcatcaata taatcaccac
      481 aatctttgcc gcacccgctg caggaaacat ccataccata gtggcgatca gccagccgga
      541 aatgatagcg ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt
      601 gacaaatgca tcgcgagttt cttcattgaa gataggcata tattcaaatg cccacgccgc
      661 aatacctgtc ccgagaatat tacccagcag cacgacgccc cataaccgca taagtaagcc
      721 aacgttgctc attgtcggtt tttgcatgac gggtagtaca gcagtcacgg tgttttcggt
      781 aaataattgc tggcgggcca taatgacgat aataaaacca aaggtataac ctagattctc
      841 cagcaagaag ctgcccggca caccttccag ttcgacatga aatatccctt ttgccagtaa
      901 tgaagcgccc atcgacagac ccgccgcaat ggctgaccac agtagtgcca ttgcgtcgcg
      961 ttccagctct ttttcaccat cctggcggat atgctcatga attgccatcg cccgggaggg
     1021 gagtcgatct tcatctattt ctattttttt gccacgctct ttttcttcac tctcaacttc
     1081 aatttcgtcg ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc
     1141 acgtatagct aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat
     1201 gtagaaaagg ccgcgtttgc ctttttctgt ttccatagaa tcaagtagcc tacagggcgg
     1261 cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct
     1321 gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac
     1381 tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc
     1441 tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg
     1501 acccgttaga agggttcaac cgcaccatgt acaacttcaa cttcaatgta ttagacccgt
     1561 atattgttcg accggtcgct gttgcctggc gtgattatgt tccacaaccg gcgcgtaacg
     1621 gtttgagcaa ctttaccggc aaccttgaag aacctgcggt gatggttaac tacttcttgc
     1681 agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac accattttgg
     1741 ggatgggtgg ttttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac
     1801 ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatgggcct tacgtccagt
     1861 taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggcg gatggtcttt
     1921 acccggttct atcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggga
     1981 tagaaactcg tgcgcagttg ttggattccg acggtctgct gcgtcagtcg tccgatcctt
     2041 atattatggt gcgcgaagcg tacttccagc gtcatgattt catcgctaat ggcggcgaac
     2101 tcaaaccgca ggaaaatccg aacgcgaaag cgattcagga tgatttaaaa gatatcgatt
     2161 ctgaataaga aataaataaa aaaggtgagt cgcaatactc accttttttg ttgttatccc
     2221 tcaattacca caagtaaaag gagataaacc gacaatgacc cggacgtaat tatgtaaaaa
     2281 tactgatgtt cgtcactgac tatttccgat attcagctgt tgaaaaatca aaaaactgga
     2341 aaaataattt a
//
LOCUS       DAZFFG010000273         2242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFFG010000273 DAZFFG010000000
VERSION     DAZFFG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.275"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            23..1750
                     /locus_tag="ACHJ7J_005036"
     CDS             23..1750
                     /locus_tag="ACHJ7J_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO5003117.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPQPENKESQQQQPSTPTEQQVLAA
                     QQAAIKEAEQSAAAAKALAQQEVQQYSDKQALQGRLQEAPTFARAAKAKATHIANPGT
                     ARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYF
                     PSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLV
                     FLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAE
                     INAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIE
                     LMIKKQRESGVTLSTFGVGDDNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQ
                     TLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLF
                     ELTLNGQKASIDKLRYAPDNKLAKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTIN
                     APSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGVDPQGYRAEFIRLIEL
                     ADGVTDISQ"
     gene            complement(1821..>2242)
                     /locus_tag="ACHJ7J_005037"
     CDS             complement(1821..>2242)
                     /locus_tag="ACHJ7J_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFO5003118.1"
                     /translation="IINSFNSFNSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQY
                     LSQSCGAFVCMAAQEVIEQMESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVN
                     RNCYLDKYGDANINHYYRNLEIKHSQPKNRASSKRVS"
BASE COUNT          647 a          456 c          534 g          605 t
ORIGIN      
        1 agtttattta aaaggatttt atatgcgaaa taaaaatata atcatgttgc ttatgagtag
       61 tttgattttg tcaggatgtg ggccgcaacc tgagaataag gaaagtcagc aacaacaacc
      121 cagtactccc acagagcagc aagtgcttgc cgcgcagcaa gctgcaataa aagaggctga
      181 gcaaagcgcc gccgccgcga aagccttggc ccagcaagaa gtgcaacaat attcagacaa
      241 acaggcttta caggggcgat tgcaggaagc gccaacattt gcaagagcgg ctaaagcaaa
      301 agctacacat atcgcaaatc caggaaccgc tcgctaccag cagttcgatg ataatccggt
      361 taagcaggta gcgcaaaatc cgttggcgac gtttagtctt gacgttgaca ctggcagtta
      421 tgcgaatgta aggcgtttcc tcaatcaagg gctgttacct ccgccagacg ctgtgcgggt
      481 ggaggagata gtcaattatt tcccgtctga ttgggatatc aaagacaaac aatctattcc
      541 ggcctctaag ccaatacctt tcgctatgcg ctacgaattg gcacctgcac catggaatga
      601 acagcgaaca ttgctgaaag ttgatatcct ggcgaaagat cgcaaaagtg aagagttacc
      661 agcttctaat ctggtctttc ttatcgacac ttctggttca atgatttctg atgaacgttt
      721 gccacttatc cagtcttcgt tgaaattatt ggtcaaagaa cttcgtgagc aggataacat
      781 tgccatcgtg acctacgctg gcgactcccg tattgcattg ccttctatct ccgggagtca
      841 taaggcggaa attaatgccg caattgattc gctggatgcc gaaggcagta ccaatggcgg
      901 tgccgggctg gaactggctt atcagcaggc ggcgaagggg tttattaagg gcggtatcaa
      961 tcgcatttta ttggccactg acggtgactt taacgttggt attgacgatc caaaatcgat
     1021 tgaattaatg atcaaaaaac agcgggagtc cggtgttact ctgtcgacgt ttggcgtggg
     1081 ggatgacaat tacaacgagg caatgatggt gcgaattgcc gatgttggca acggtaacta
     1141 cagctacatt gataccctct ctgaagcgca gaaagtattg aacagtgaaa tgcggcagac
     1201 gttgattacc gtagcaaaag atgtcaaagc gcaaattgag tttaaccccg cgtgggtaac
     1261 ggaataccgt cagattggtt atgaaaagcg ccaacttcgg gcggaagatt ttaataacga
     1321 caacgttgat gcgggggata ttggcgcagg caaacatata acgttgttat ttgaattaac
     1381 gctgaacggg caaaaagcat caattgataa gttacgctat gccccggata acaaattagc
     1441 gaaatcggac aaaacgaaag aactggcctg gttaaaaatt cgctggaaat acccgcaggg
     1501 aaaagaaagt cagttagttg aattcccgct ggggccaaca ataaacgcgc cctctgaaga
     1561 tatgcgtttt cgcgcagcag tagctgcata tgggcaaaag ttacgcggtt ctgaatacct
     1621 gaacaatacc tcctggcagc agatcaaaca gtgggctcag caggcaaaag gggtagatcc
     1681 acagggttac agggcggaat ttattcgcct gattgaactg gcggatggtg tgactgacat
     1741 cagtcagtga tgacttttta gcaaactatg ttcgaccagt cagcatattt gctgactggt
     1801 cgaattaatt aacaatgatg ttaacttact cttttgcttg atgctctatt tttgggttgt
     1861 gagtgtttta tttctaagtt tctataataa tgattgatat ttgcatcgcc atatttatct
     1921 aaataacaat ttctgtttac cagtcgatgt tgaaaatcaa tttcgtactg ctcttctgct
     1981 gaatattttt taaatctgtc agcatagttt tttaataacg tatagggggc gctgtcagaa
     2041 ttgctttcca tttgttcaat cacttcctgg gctgccatgc acacaaatgc accgcaactt
     2101 tggcttaagt attgctgaag atcggcatgt aaaaatatta tctctggttc agttaaatga
     2161 tctgttatat tcttattatt cggcgtttct ctcgtggggt cacaattgaa actattgaaa
     2221 ctattgaaac tgttaatgat ac
//
LOCUS       DAZFFG010000274         2162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFFG010000274 DAZFFG010000000
VERSION     DAZFFG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.276"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(13..291)
                     /locus_tag="ACHJ7J_005038"
     CDS             complement(13..291)
                     /locus_tag="ACHJ7J_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5003119.1"
                     /translation="MIRCHLNRLMGERRLKVADVVRDTGLPRSTVTALWKDNATRIEL
                     PVLSILCHYFNCDVSELLEFTSDENHVELSRPAKGLNNFSHRVWFMRK"
     gene            complement(558..1970)
                     /locus_tag="ACHJ7J_005039"
     CDS             complement(558..1970)
                     /locus_tag="ACHJ7J_005039"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5003120.1"
                     /translation="MNTNPLNTNFLKLKFPPLFEEVTQATQAITHAPAELITGVQLSV
                     MSLAAQGVVVFEHSDGRRSPVSLYSIVLAESGERKTAVCNLLQKPIQDFQKKQLKNYE
                     EKINVYEADLQSWAVLNKTITRQIRKNIEKGGDSVSEQEKLRKHYINKPTPPKRPKLL
                     YSDATPEAIIQGLVESIGTSGLISDEGGVIFNGRAMGNTPLFNQLWDGGIVDVERKGH
                     RLNVDNARLMALIITQRNELLLFRKKHGARVQGNGFFARCLWSVTTSTQGIRMHQPDV
                     PVDTSVLDKFNKRVEELLEQTLAGTTPCVLHFSADAERRFAQYQNEIEQRILTEKGKH
                     DALPGILSKLPENAVRLATLMHFFYEFKGDEIDVATLNYIFMIMEYYYQQAVNILTAS
                     LSSDEEDAKRLWEWLMTGHFSTSTSVAKSEIRRYAPYQLRDVVRYNKALKYLEDIGRI
                     YIKKYKNGNGTVSQVIDINR"
BASE COUNT          612 a          496 c          403 g          651 t
ORIGIN      
        1 aatgattttg tcttatttac gcataaacca gacacgatgt gaaaaattat ttaaaccctt
       61 tgctggacga gataattcaa cgtgattttc atcagaagta aactccagta actcactgac
      121 atcacaatta aaataatgac agagaatgct tagcactggc aactcaatcc gggtggcgtt
      181 atctttccag agcgctgtga cagtgctacg cggaagaccg gtatcgcgga ccacatcggc
      241 aacctttaat ctgcgttcac ccatcagtct gttcaggtgg cacctaatca tcaatcactc
      301 ctgtaataca gaaactcaaa atgtcattca gcaaagcatt ctgcttcaca gaaaataaaa
      361 agcatcagca cgaacctgac agtcgtcaga cctgggttaa aatgttaacc gtaaggttta
      421 cactgttgtc gtctggtttt tcctatacgg atacaaagtg tgcttttcat gcaggaaaat
      481 tttcaggcaa tacccccccc tgcgatttct gctcttcgga gcaggaaaag cagaggagta
      541 gttatgcata tcttacatta acgattgata tcaatcactt gactgacagt accgttaccg
      601 tttttgtact tttttatata tatcctacca atatcctcaa gatattttaa cgctttgtta
      661 tagcggacta catcacgcag ctggtagggc gcataacgtc ttatctctga cttcgcgact
      721 gaggtagacg tcgagaaatg gcctgtcatt aaccactccc agagtctttt agcgtcctcc
      781 tcatcggaac tcaggctggc agtcaaaata ttgactgctt gctggtaata atattccata
      841 atcataaaaa tatagtttag tgttgctaca tcaatctcat cacccttaaa ttcataaaaa
      901 aagtgcatca gtgttgccag ccttacagca ttctcaggaa gctttgataa aattcccgga
      961 agtgcatcat gctttccttt ctcggttaat atcctttgct caatttcatt ttgatactgt
     1021 gcaaagcgcc tctccgcgtc agcagaaaaa tgcagaacac aaggagtggt tccggcaagc
     1081 gtttgttcaa gtaattcctc aactcgtttg ttaaatttat ccagaacgct ggtatctacg
     1141 gggacatcag gttgatgcat tcttataccc tgcgttgatg tagttacaga ccacagacag
     1201 cgggcaaaaa aaccatttcc ctggacacga gcaccatgct ttttcctgaa caggagtaac
     1261 tcatttctct gggtgattat aagcgccata agtctggcat tatcaacatt cagacgatgt
     1321 cctttacgct ccacatcaac aatcccacca tcccagagtt gattaaaaag aggtgtatta
     1381 cccatcgccc gcccattaaa aatcacaccg ccttcatcgg agattaaacc tgacgtacct
     1441 atgctttcaa ccagtccctg gatgatagcc tcaggtgtcg catcactgta taaaagcttt
     1501 ggtcgctttg gaggggtagg tttattaatg taatgctttc tcaatttctc ctgctcagaa
     1561 acactatcac ctcctttctc gatatttttc ctaatctggc gggtaatagt tttgttcaag
     1621 acagcccatg actgcaaatc agcttcatag acatttattt tctcctcata attcttcagt
     1681 tgctttttct gaaagtcttg tatcggtttc tgcaggagat tgcacactgc cgttttcctc
     1741 tcccctgatt cagccagtac aatgctgtaa agagacacag ggcttcttct tccatcactg
     1801 tgttcaaaca caaccacacc ctgagcggcg agtgacatta ccgacaactg tactcctgtg
     1861 atcaactctg ccggtgcatg cgttatggct tgtgtggcct gtgttacttc ctcaaaaagt
     1921 ggtggaaatt taagtttcaa gaaatttgta ttaagtggat tagtgttcat ttctatctcc
     1981 tgtgaatcat ttggatgaag agcaggagag caacttaaac gttaaaattt atgctgtgaa
     2041 gccacctcag tggaattaga ttgcatcgga ctgaattagt ttaaaacatc aatctgtaag
     2101 catcatcgaa attgaaaatg atttaacctc acgactatta ataattactt atcaatttat
     2161 tt
//
LOCUS       DAZFFG010000275         2156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFFG010000275 DAZFFG010000000
VERSION     DAZFFG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.277"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(769..903)
                     /locus_tag="ACHJ7J_005040"
                     /pseudo
     CDS             complement(769..903)
                     /locus_tag="ACHJ7J_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(873..1016)
                     /locus_tag="ACHJ7J_005041"
                     /pseudo
     CDS             complement(873..1016)
                     /locus_tag="ACHJ7J_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1101..1298)
                     /locus_tag="ACHJ7J_005042"
     CDS             complement(1101..1298)
                     /locus_tag="ACHJ7J_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5003121.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREGIRTLRQLQLLHQNR"
     gene            complement(1318..1806)
                     /locus_tag="ACHJ7J_005043"
     CDS             complement(1318..1806)
                     /locus_tag="ACHJ7J_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5003122.1"
                     /translation="MKLSLTLEADSINVQALNMGRIVVDIDGVNLTELINVVCDNGYS
                     LRVVDESDRASTDCTLPFAALTGIRCSTAHITERDNAWLFSLSHQTNDTGESEWIHFT
                     GSGYLLRIGAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDAGAECLPGLPTF
                     DW"
     gene            complement(1803..>2156)
                     /locus_tag="ACHJ7J_005044"
     CDS             complement(1803..>2156)
                     /locus_tag="ACHJ7J_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5003123.1"
                     /translation="VREVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQH
                     IEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATGLMTRSNYR
                     TVNNITLGKHPEAKQ"
BASE COUNT          575 a          525 c          505 g          551 t
ORIGIN      
        1 tttttataac caatgaaatc tacaacaagc aactgatgga taatccgata ttggtcgcat
       61 ttaatggcat ggtagcaaag ttttcatcac atacctcctt tgcaccagaa taaacgccag
      121 agatctgaaa ttgcgagtac actacagaaa ttgtactgcc ggtcccatca gcataaaacc
      181 atagttacac aggtaatcat cattattcgc caagataata aatattattc ggtagaatag
      241 cgttctcagg tggtcagtaa aattgtcttc aacaagatgt ctgcgcatct cgtctgctag
      301 ccctacataa ctgctctcga ccgaaactat ttattgaatg ttcaccaaga tgggcgtgga
      361 gacaaaatga cgtcttttga tattagctac ctgagccaca tggtcaaaac gttcaagtag
      421 catagcgaaa ccaccctgcc cacgtaatgt ggacgatgca gcttccagcc agagcaaact
      481 tgggaccatc gactacaaaa tgaagaatac tgaactgctt ttctggcagg cctatatact
      541 gcacggatat gggtgatttc agacacaaaa aaagccgctc ttgagcgact cgatttgcat
      601 acggtgtggt gcgaaggccg gactcaaaca tgtgacttaa cctcatgatt taaaattgtt
      661 aataataaac aatatcatct tataccaaca cagataccaa cacgaaaaat cactggtttt
      721 cggcatcgaa ccaggacaat cagctcctga ttgagatacc gagctctgtt actcgtcatc
      781 ttcctcgaca ggcttcattg catcaagtag tactcgcacc atcgcgatag aaccaatccg
      841 gataaaagca tcttctccat ctatatctgt taactgatat cgctcacttg atgccggata
      901 agtgtgtcga tttgcagcag tgtactcttt tgtacagcca gaatgtggct gactgaggtg
      961 gagcagataa tgtccccgtt gaccacttcc ggccgggagg ctgtgatttc agacataatt
     1021 gatattcctt tttgcagata aatgaaaacg ccagccctgt tcaggctggc gtcagtggaa
     1081 tgaagtatgg tgagtaatat ttatcggttc tggtgcagaa gctgcagttg gcgcagggta
     1141 cggataccct ccctggcctg ttcgatacag ggcaacagtg ctgccgaatc cgttttatcc
     1201 tcatcgttgt caaagataat tcccgattcg cagtcgatat tgtcctgcag ccacgcaatc
     1261 agaatatcca gcgccgtttc tgtggttaat aatttcatcc agtgtctttt gtcctgatta
     1321 ccagtcgaaa gtgggtaaac ccggcagaca ttctgcgccg gcgtccagat gaatgagact
     1381 gacgccataa cgccgggtaa gtgtgacaac cagacgacgg aacgtttttg acaggcccag
     1441 ccgtttaagt cgcagaaccg ggtacgacca cgcaccgata cgtaacagat atccgctgcc
     1501 ggtaaaatga atccattctg attcgccggt gtcatttgtc tgatgcgaca gcgagaacag
     1561 ccaggcgttg tccctttctg tgatatgagc ggtactgcag cgtatgccgg taagggcggc
     1621 aaatggtagc gtgcagtctg ttgaggcccg gtcagattca tcaacaacac gaagggagta
     1681 accgttatcg cagaccacat taatcagttc agtgagattt acaccatcaa tgtcaacgac
     1741 aatgcgcccc atgttcagtg cctgcacgtt aatgctgtcg gcttccagtg tcagggaaag
     1801 tttcattgtt tcgcctccgg atgcttcccc agggtaatgt tatttaccgt tctgtagttg
     1861 ctgcgggtca tcagacctgt tgcccggcgt gcccggagaa tatcaatgct gttgattaac
     1921 tgcgacgaag ttcctgcgct gaatcccggc tggtcggtac gcaccagcgc gtatttttcc
     1981 acgagaaagt tcaccgcatc acacagtgaa atgcctgcct caatatgctg ctcaatcaca
     2041 cgttcatcag cgaacggtgt gtcattcagc gtgaggccgt agtgctggtc cagcaaccgg
     2101 gtgagcagtg tctgccagat ggtgacggga gacgggcagc gagatacctc ccgtac
//
LOCUS       DAZFFG010000276         2078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFFG010000276 DAZFFG010000000
VERSION     DAZFFG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.278"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..724
                     /locus_tag="ACHJ7J_005045"
     CDS             <1..724
                     /locus_tag="ACHJ7J_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5003124.1"
                     /translation="FLMNTTSTRYDRFAIRLTQIISRLLSGESLDLKNLAEEFGVSER
                     TLQRDFHQRLLHLNIVSENGRWRLDGKRFTDSSLDVLAFMRNTGISQLFPSPDRRLMH
                     YLTGETSYSPCLIWPSSHSDSAVSSECFQRLIQAICQQNCIHLLTGNDSHAVFEPYRL
                     IHTEGSWYLVACQAEIIRVIPVASINQVKLTTESFVRRDDICHLTAGERFISSLPHVP
                     YIHDVMHSANHRSYKKYCSSDR"
     gene            787..1257
                     /locus_tag="ACHJ7J_005046"
     CDS             787..1257
                     /locus_tag="ACHJ7J_005046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003125.1"
                     /translation="MNIVKIKASFYILMLLSSSSLAGQENWDLKCTLDTGGVMTLSHK
                     KDIVYITYKSQDKSSDKGSVVIKLNTHSGQAQQSITANDVTDNRVFVFRGTGENIEGA
                     MAITYEEYKGQSDAHISVMNPMGKEIESHACLTNTINARSNLLYTGIEHMPFIH"
     gene            1334..2053
                     /locus_tag="ACHJ7J_005047"
     CDS             1334..2053
                     /locus_tag="ACHJ7J_005047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003126.1"
                     /translation="MSKRKVLTGLFISCSIFILTGCDNVSASDNQEIIKSQVLSNIDS
                     TRTLGTALEHRQRCESFKWEDGTDEQGRQTVTYTCDMSASATNSIWKREMTRFANGYA
                     SLIESAKRSIDQYNEACVANGSDPYCFKKEPDNSKLKEGLSAAKKQADNEIIRVKQII
                     IWSVLPDTEQPVQLLSAKYYFDFNNDSQSWASQQGDNLLKDIYNNNENNNPIIGLIGY
                     EAAKINCSYRADTAACNYLKW"
BASE COUNT          659 a          423 c          423 g          573 t
ORIGIN      
        1 ttttctgatg aacactacat cgactcgtta tgaccggttt gccatcaggc tgacacagat
       61 tatcagccgg ttactgtcag gagagtctct tgacctaaaa aacctggcag aagagtttgg
      121 agtatctgaa cgaacgctac agcgagattt ccaccagcga ttactccacc ttaatatcgt
      181 tagtgagaat gggcgctggc gacttgacgg gaaacgcttt acagacagtt cgttagatgt
      241 tctggcgttt atgcgtaaca ccggaatctc tcaattattt ccgtcacctg atcgcaggtt
      301 gatgcattac ctcacgggtg aaacgtcata ttctccctgc cttatctggc cttcatcgca
      361 ttctgactcg gctgtgtcat cagaatgttt tcagcgactg atacaggcga tctgtcaaca
      421 aaactgcatt catttgctga ctggcaatga ttctcacgcg gtctttgaac cttatcgtct
      481 tattcacacg gaaggaagtt ggtatttagt tgcctgtcag gccgaaataa tcagggttat
      541 tccagtagcc agcatcaacc aggtgaagtt aaccacagag agttttgtgc gcagagatga
      601 catctgccat ctcactgcgg gtgagcggtt catctcatcc ttacctcatg ttccttatat
      661 ccatgatgta atgcactcgg ctaatcatcg gtcatataaa aaatattgct catcagatag
      721 atgagtatta aaagaggagc tgcttcatca attaagaata gtctccccac ttaatcagtg
      781 ggctatatga atattgtaaa aataaaagca tctttttata tactaatgtt gttaagcagt
      841 tcttcactgg ctggtcagga aaactgggat ctaaaatgca cacttgatac tggtggggtt
      901 atgacactga gtcataaaaa agacatcgta tatattacct ataaatcaca ggataaaagt
      961 tctgataaag gcagcgtagt catcaaactg aatactcatt caggtcaagc gcagcagtcc
     1021 atcaccgcaa atgatgttac tgacaataga gtctttgtat ttcgaggaac tggagagaat
     1081 atagaaggcg ctatggctat cacttatgaa gagtacaaag gtcagtctga tgctcatatc
     1141 agtgtcatga accctatggg taaagaaatt gaaagtcacg cctgtctgac caatacgatc
     1201 aatgccagga gcaatttact ttatacaggc atcgaacata tgcccttcat tcattaaaac
     1261 cagattaacc atcttatcag acctgattta gtcaaggtca cgtattttac caaaacttca
     1321 aaaggaatca ctcatgtcta aaagaaaagt acttactgga ttgtttatca gttgctcaat
     1381 atttattctt actggctgtg ataatgtatc cgcttcagac aatcaggaaa ttataaaatc
     1441 acaggtatta tcaaatatag actcaaccag aacccttggg actgcattag agcatcgcca
     1501 gcgatgtgaa tcgtttaaat gggaagatgg aaccgatgaa caaggacgcc agactgttac
     1561 ttatacgtgt gatatgtcgg catctgcaac aaatagcatc tggaaaaggg aaatgactcg
     1621 gtttgctaat ggctacgcca gtttgataga aagcgcaaag cgctctatag accaatacaa
     1681 tgaggcctgt gtcgccaatg gaagtgatcc ttattgcttt aagaaagaac cagacaattc
     1741 aaagttgaaa gaaggtttaa gtgcagctaa aaaacaggct gataatgaaa tcatcagagt
     1801 taagcaaatt attatctgga gcgttttgcc agataccgag caacccgtcc aactgctctc
     1861 agcaaaatat tattttgatt ttaataatga ctctcagagc tgggccagcc aacaaggaga
     1921 taacctccta aaagatattt acaacaacaa tgagaacaat aaccccatta taggtttaat
     1981 cggctacgaa gcagctaaaa taaactgttc atatagagca gacactgctg cttgtaatta
     2041 tttaaaatgg tagccatgaa taaacaaagc tttggtaa
//
LOCUS       DAZFFG010000277         2067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFFG010000277 DAZFFG010000000
VERSION     DAZFFG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.279"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            95..904
                     /gene="yqeI"
                     /locus_tag="ACHJ7J_005048"
     CDS             95..904
                     /gene="yqeI"
                     /locus_tag="ACHJ7J_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5003127.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIEVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRD"
     gene            897..1379
                     /gene="yqeJ"
                     /locus_tag="ACHJ7J_005049"
     CDS             897..1379
                     /gene="yqeJ"
                     /locus_tag="ACHJ7J_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO5003128.1"
                     /translation="MINYKKNLLFIVVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNQKNKTGTVALSGTWQQGNRESKSIRRNIEYTWVENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1412..1492)
                     /locus_tag="ACHJ7J_005050"
     CDS             complement(1412..1492)
                     /locus_tag="ACHJ7J_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003129.1"
                     /translation="MKDVDQNFDALYWHILQAYLILLFYD"
     gene            complement(1528..1953)
                     /gene="yqeK"
                     /locus_tag="ACHJ7J_005051"
     CDS             complement(1528..1953)
                     /gene="yqeK"
                     /locus_tag="ACHJ7J_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO5003130.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRYVNVNSVSKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLILSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          716 a          362 c          350 g          639 t
ORIGIN      
        1 aaatataaaa ttaatatata tttatgccgt aaataccaat atacttagca aactatgtga
       61 tttccatttc gattgattta gtgtttattg acgtatgtac tggattatta acgataatat
      121 cgagttctgg cctgagcacc gaaaattaat atcggtacat aacgccgatc ttaacgtcgt
      181 tctgacaacg ccagccagtc gttgtttatc acttctactt gaagcttttc ctgatgtggt
      241 tgcacaacaa gattttttca ccagagtctg ggaagaagaa ggtatgcgtg tgcctactaa
      301 cacgttatat cagaacatat ccattatcag acgcggattt cgcgctgttg gtgatactac
      361 ccactcgctt attgcaaccg tgccgagaag aggattcaag atccataatg acattaacat
      421 acaaaatcat gtaataaact cgtcaacaga cgcacataca cacaatgccc cacctgccat
      481 agaagttaat gcgggataca aagagagcat tggtggcgca aagaatttca ataacaaaat
      541 cctcaaacat ataaaaagtt atctaattat gttgagcgca tttgtcatag gcgcatattc
      601 tgcatattgg ctatggaata ataatcaacc gaagccattt ttcaaggatt acaagactgt
      661 tgcggaaatt aatggctgtc attttaatgt aacagaagat acaattgatg ggttgaaaga
      721 gttcgataaa tataaaacac ggatactgga ttcggggatt aactgtaaaa aatatccgtg
      781 gttatacttc cctcttgcaa aatcctcacc ggggatgatt gttatggcat gcaataaaaa
      841 ctataaccaa catgaagtgg caggctgttt aaccctgtct taccgtgagg ttaatcgtga
      901 ttaattataa aaaaaactta ttatttattg tagtttttat cagtgggttt attctcttca
      961 ctgtctattc ttacactgca gaaaaaatga tatataacga aacatgcact gcaaattggg
     1021 tgatttttaa tgaccagggg cgagcaaatc ttactatcga ctttatgtat aaccaaaaaa
     1081 ataaaacagg aactgttgcg ttgagtggga cctggcaaca aggtaataga gaaagtaaat
     1141 caatacggcg aaatattgaa tacacatggg ttgaaaacta tgacacagcc catttaactt
     1201 caaaaaaagt taataagttt gagattatgg atcaagtcga tgatgataga cttgctgaac
     1261 ttattcctga tttttatgtc tttccagaaa aaagtgtaag ctataatata ctaaagcaag
     1321 gtaaacatgc ttttattttg agtattggta acagagcaat aatgcattgt gcaaggtaga
     1381 ctaaaataga taacaaaaag atggaacctc gttaatcata aaataataaa attaaatatg
     1441 cttgcagtat atgccagtat aaagcatcaa agttttgatc aacatctttc attttagaca
     1501 tcaccttgca atgtaaaata tcatgtatca acaacatctg gtgcatatcc atgagtcgaa
     1561 ctcaacattc atcttagctc gattattatt ccttcgtaca gtcgatgtaa acaatacaga
     1621 aagaggatta ttaagaacca gtttactcag tgaatcaata gaggaaagga ttaacgtttc
     1681 tttaaaagaa ttaatttcat catctgttaa actaaactca tcattgacag atcgtgagat
     1741 ataactgttt ttaactttac tctttacgtt gactttactg acagaattaa cattcacata
     1801 tcttgaattt aatgcccatg atgttgtttg cgtgagaacc ttctcagcat taaggaattt
     1861 ttttaacgga atccttggtt tctttttcga gtccgaatta actatatcat gcatatagac
     1921 catttcatga atctgcgaaa actcaatgtc cattatatac ctcacatttt taccgtgact
     1981 cgatgttact gttcaataat caccatccat caatactaaa attaataccc caaatgtgcc
     2041 gataacaaat atagtcattc tacgtaa
//
LOCUS       DAZFFG010000278         2052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFFG010000278 DAZFFG010000000
VERSION     DAZFFG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.280"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            208..>2052
                     /locus_tag="ACHJ7J_005052"
     CDS             208..>2052
                     /locus_tag="ACHJ7J_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO5003131.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKGITVR
                     IDNINGKKNNSNSSDVFIPYVNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHIL
                     RTVNSLNVGEKLNDDNLADHSIFTNEFSVIIPPSYYDATSVVNANNGVREKLFESDSK
                     VKDIVDDMSNHDVESEKDIFVIGGMIEKLDSLVDESFSENTDNIQTVKDLLTQLTDGM
                     ELFALRDIVAFPSSIIAKLLKSTLNSDHELVMRALDTYLCYFRNKDLSNDTQIINFFH
                     ALFLKKPELMVSENYRFIQFIDLLFENGSVEEKNLAFDLYHNYLALPEIKQFVTEEIK
                     LNFNEQQGLLDKDNKCYILLSSDNSGRVMSLSHQALISMLEPEVKKKTIWNNYSIYPS
                     LQDTHEVVRDDPETICTRAFPLFAKGWEYAQRNKKHQLILNALGFKGYIRDVFMSAIM
                     RKTYFVPECNNQPTELNSSFSSLMTDSDQWQQHSLKDKHYANLLTMLDLKEASESDKS
                     KIFFCLSAVFANISHSNVFNGIPDASKTLKRYAFALLAKAHSLDESMIS"
BASE COUNT          690 a          338 c          384 g          640 t
ORIGIN      
        1 taaattaact atcaaaaatc gctcatttct gcttattgat agaaagattg attgccagcc
       61 tgtttagagc ggtgcagcag caaactttca taaccaggtt gatcctgaat ttgcagtacg
      121 aaaagttaaa aatgagcgat ttctgtaaga ttccccattt tgccaggctt atgattcgca
      181 gatcattcaa gtgcaaagga gcgatgtatg gattgttcaa aatgcaatgg ttatgcgacg
      241 atgttattaa atatggtgca gggttctgat ccggttaatt tactggaatt acatggtttc
      301 cttgaacatt tcgcatatta tgtttcattt ggcaaattta atgctggcca ccagcgctat
      361 aatgcattta aaaaattcgt cagtgagatt tcggagatca gcgcaaacga tatcaatatg
      421 acgattaaaa ccgggcaatc cagacatgaa aatgttatct ctataaatat gaatgatgca
      481 attcctcgtg atgagaaagg gatcaccgtc agaatagata atattaatgg aaagaaaaat
      541 aactctaatt catcagatgt ttttattcct tatgtaaata catttcccga cctcaaaaat
      601 aaaatactca ggatgaaaat tgaactgact gaaggtagtg gtttttctaa aagtctttct
      661 gacagtcaga ttgagatgca tattctgagg actgttaatt cgctaaacgt tggcgagaaa
      721 ttgaatgatg ataatcttgc agatcattct atattcacta acgaattctc tgttattatt
      781 ccgccatcct attatgatgc gacttctgta gtaaacgcca ataatggtgt gagagaaaaa
      841 ctatttgaaa gtgatagtaa agttaaagat atcgtcgatg atatgtctaa tcatgatgtg
      901 gagagcgaaa aagatatatt tgttatcgga ggaatgatag aaaaactgga ttctctggta
      961 gacgaatctt tcagtgaaaa tactgataac atacaaacag taaaagattt gttaacgcaa
     1021 ttaactgatg gcatggaact cttcgcgctg cgggacatag tggcattccc atcgtccata
     1081 atagcaaaat tattaaaatc cacacttaat agcgatcatg aacttgttat gcgcgcattg
     1141 gatacgtatc tgtgctactt cagaaataaa gatctgagta atgatacgca aataattaac
     1201 tttttccatg ctctcttttt aaagaaacca gagttgatgg tttctgaaaa ttatagattt
     1261 attcaattta ttgatttgct ttttgaaaac ggaagtgttg aagagaaaaa cttagcattt
     1321 gacctttatc acaattatct tgcattacct gaaataaaac aattcgttac tgaagaaata
     1381 aaattgaatt tcaatgagca gcaaggattg ttagataaag ataataaatg ttatatcctg
     1441 ttatcatccg ataactctgg gcgagtaatg agcttatcgc accaggcgtt aatctcgatg
     1501 cttgagccag aggttaagaa aaaaactatc tggaataatt actctattta tccatctttg
     1561 caggataccc atgaagttgt cagagatgat ccagaaacga tttgtacgcg tgcatttcct
     1621 ttatttgcaa aaggatggga atatgcgcag agaaataaga aacaccaact gattctcaat
     1681 gctcttggat tcaaaggtta tattcgtgac gtatttatgt cagcaataat gcgaaaaaca
     1741 tactttgtgc ccgaatgcaa taatcaaccg acagaactta attcatcatt ttcctctttg
     1801 atgaccgatt cagaccaatg gcaacagcat tctctgaaag ataaacatta cgctaatcta
     1861 ttaacgatgc tggatctcaa agaggccagt gaaagtgata agtctaaaat atttttctgc
     1921 ctgtctgccg tatttgcaaa tatttctcac agtaatgtct ttaacggtat tcctgatgcg
     1981 tcaaaaactt taaaacgtta tgcatttgct ttattggcga aagctcattc tttagatgaa
     2041 agtatgataa gc
//
LOCUS       DAZFFG010000279         2043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFFG010000279 DAZFFG010000000
VERSION     DAZFFG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.281"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            102..314
                     /locus_tag="ACHJ7J_005053"
     CDS             102..314
                     /locus_tag="ACHJ7J_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003132.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
     gene            386..985
                     /locus_tag="ACHJ7J_005054"
     CDS             386..985
                     /locus_tag="ACHJ7J_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO5003133.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQFGFDYWTPVSGAITPRERELVSGFVEYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFDAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     DFQQVYKAVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            985..1254
                     /locus_tag="ACHJ7J_005055"
                     /pseudo
     CDS             985..1254
                     /locus_tag="ACHJ7J_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
     gene            1271..1819
                     /locus_tag="ACHJ7J_005056"
     CDS             1271..1819
                     /locus_tag="ACHJ7J_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5003134.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGCGRPRS
                     MFYQLFSSKKLTKTAVNEFLRRMQKAGIEKTELEAFFREMINGKQKSWLAHCTDEEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMSKLRMAEQLNEYHPDWTLITCRRRIDLWL
                     KITEFMLYKPILITLKKLQNEQ"
BASE COUNT          601 a          397 c          490 g          555 t
ORIGIN      
        1 attaaataat caaatatacg acgtgaaata aatatttttc agattaatat ttttgtctct
       61 atgtggatat aaccgtttgt acttataaac ctggaggcat cgtggaaaaa ataaagaaac
      121 tatttagtag caaatatgca gtcatacgtc gtgatgacct gtcagttata gtcgaaatgg
      181 attacttccc tgaaactaaa aaatcaatga tgtatcgtaa tggtcgaaag gcaatttttt
      241 taccgatgag ggtaagtgac attatgggaa atgataaact gctggatgaa ttgcgagtca
      301 gagcatcctg ttagtattgg cattaattct ggtatactac ataacgggct gaacacccat
      361 tctactgcgc cagcggagaa ctacgatggc gcatatacaa ctggtcaaac aaacctcttc
      421 cggattactt ctcccggcga cgccggagag ttgcgatttt ctgcatcaaa tcaaaatagg
      481 tgagtggata cacgcagact ttaagcgtgt gcgtaactac gcattccata agcgtttttt
      541 caaacttctg caattcggct tcgattattg gaccccggtc agtggggcaa tcacgcctcg
      601 agaacgagaa ctggtatcag gcttcgttga gtacctgtgc gaatcagtag gtcgggaaca
      661 tactcctgcc ctgagtgatg ccgcagagca atacctgaat accgttgcga cacgcagaac
      721 ccgggatacg gcattgctta agtcatttga cgctttccgc gaatgggtaa ccattcaggc
      781 cggattttac accgagcatt tttatccgga cggtagccgc gggcgtcggg cgaaatccat
      841 cgcgtttgcg aatatggacg aaaccgactt tcagcaggtt tataaagccg tactgaatgt
      901 gctgtggaac tggatcctgt tccgtaaatt ctcctctccg gaggaagtcg aaaatgtggc
      961 cgcgcagctg ctggagtttg cgtaatggtg gatttacgta aagcggcgtg gggccagatg
     1021 tgccaggtca gaatccctgg ctactgcaat cacaatcccg aaacttcagt gcttacgcat
     1081 tacaggctgg cggggacgtg cggaatagcg acaaaaccac acgatatgca ggcagcgatt
     1141 gcctgtagct catgccacga tttaatcgac gggcgggtaa aaaccatcga ttacaccaaa
     1201 gaagaattac gcctgatgca tgcagaaggt gtttttcgca cacaagaaat ctggataaag
     1261 ggggatattt atgatttatc caacaaacac cgggaaaagc ggagagcacc ttcgcctcac
     1321 tacgctggaa agcgtctgga ttcagggaaa actgcgtatg tggggacgct ggtcgtatat
     1381 tggttgcggc aggccaagga gtatgttcta tcagttgttt tcgtcaaaaa aattgacgaa
     1441 aacagcagtt aatgaattcc tgcgtagaat gcaaaaagct ggaatagaga aaactgagct
     1501 ggaagcattt tttcgtgaga tgattaacgg taagcaaaag agttggctgg ctcattgtac
     1561 tgatgaggag gcgttatgca ttgatcgggt gataagtgag gtgctggcag aacacccagg
     1621 attgattagc gtccttcgac aacgatatga ggggcggggg atgagcaaac tgagaatggc
     1681 agaacagtta aatgaatatc accctgactg gacattgatt acatgtcgac gtcggatcga
     1741 cctatggctg aagattactg aatttatgct ttataaacca attctcataa cactaaaaaa
     1801 gttgcaaaat gagcaataaa ttgatttaat aaagctatgc ttcgcaaagc tgtatgcgtt
     1861 gcatagtctt tctagaatta gtccataaat agatgttaaa gccgccattg tgcggttttt
     1921 tattttatgg atatatcctg ttaattatta tatcaatgag tttttattat gaaatttttt
     1981 aaaaaattat tggtattttc agttctttcc atatcatttt catcaattgc agccgatact
     2041 aat
//
LOCUS       DAZFFG010000280         2024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFFG010000280 DAZFFG010000000
VERSION     DAZFFG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.282"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(152..331)
                     /locus_tag="ACHJ7J_005057"
     CDS             complement(152..331)
                     /locus_tag="ACHJ7J_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFO5003135.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            complement(398..2008)
                     /locus_tag="ACHJ7J_005058"
     CDS             complement(398..2008)
                     /locus_tag="ACHJ7J_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO5003136.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDIISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAINTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
BASE COUNT          530 a          429 c          365 g          700 t
ORIGIN      
        1 tttcttataa cacttgtttg gtaactattt tcaatacaaa tcatatttgt tagttcttca
       61 ttaacacact gcgagtgttt tgttactccc cgcatatatt ttattctctt gttcgattct
      121 atttttctta atcgaacata taattttctt gttagtcgga tttacgaatc atgagactta
      181 taatggaagg aatgagacta agcggccagg ttaaacctgt aattattgca gcaaacaacc
      241 gatatgttac tttttcttcc gagaagataa atattaaagt cgatgtcaca accagaccta
      301 tcaggtaagc taacagtaat ttattgatca cttttacctc ccctttcgat ttctctttga
      361 gcggtccttt taatccagga ccgcctcatt atgaaaacta tttaattccc tcatcattga
      421 taaggttgga catctctttc aacatgagag tcattgaagc tttcgaccaa tttagcggaa
      481 tgatagggct tggcgcttca tgcaaagttc ccgacttgtg gatatagtta tagctttcgg
      541 gaagtgccat ttctggtacg ctcctgccat ttgcaccaat catattctcg cctgttattt
      601 gggccaatga tctattcata aattgtactg agtcattcaa atactcttct tttcttgatt
      661 ctttataaac aattgctgct gattttgcat accatgagtc aaaaaaccat tctgcttctg
      721 ttgatggaat gaagctcttt tctctttttg catgcgaatt ttgatcggta tcggttttaa
      781 tatcattgaa ccaaaaattt gccgactgat aattatcctt ctcgtatctt tttataccat
      841 acggacctgc tagtttttta actattttta gaacttgctc ttttcttctg gtgtttatct
      901 ttgaaagatt tgcaggataa ataacattaa gtaaagccgc atcggcttct ctgtaatgtt
      961 tatctttttc cagataacct ggggattctc cccctaaatc aagctgtaac gttattcttt
     1021 cataaccttt atctatgagg tggttcaacc tcgttgttga aagagtttca tccagttcat
     1081 tgactttagc ttcgcgtagt aaatcagaga caaaaacaga gtctttcttc gagagtaggt
     1141 ttgacaaacg ttccagtcca gaggtcacca gtgcaacgga agatgtgttt aatcgggcat
     1201 cttcctccca cgctccggaa tcttccataa catagaaatt ggctttatcc agataagcaa
     1261 tcagcagcgc aaccgatttt agtcggtcac ctttttgcca atcttcagcg ttaatagtac
     1321 cagtattgat tgcttgtata aggagatcaa gataaagacc taacgcatca ttttgtttat
     1381 ggttccacag ctgcggcttg ccttcttcct gcacatctgc catcacgggg gagttgctgt
     1441 caaatcggat atgcacagca ttcatttgcc cgggaatacc atccagtcgt tttgggttgc
     1501 taatgatgtc ctgcatccgc ttaatctggt cgggcgtaga catataatcc cacagggtga
     1561 gcagaacctt ttttgcggct acactatttc cctggtctga tactaatgcc atatagcccc
     1621 ataaactatc cctcagccag acagcatcat agtgggtttc tgccgctgtt ttgtcatctt
     1681 cgaaagtaga agcaagaata aacgttcccc agggtttctc cgtcaacgtt aactttcgtg
     1741 tgttttctgt taatgttagc aatccttgaa tttcgtcttt ggtatagtgg ctctttattt
     1801 cattttgtaa tttggctgag tgaatcagaa gacgataatt aggatactta tctggattta
     1861 acgataaata acttttatct gttgctgccc ccgtggaatt aagaatgtta aatacagatt
     1921 cactcgtcac ttctgcccct gtcgcctggc atgtgccgca cgaaaatata ccagcgagca
     1981 gaagagttaa tgcagatttc cctttcataa aatattccat caat
//
LOCUS       DAZFFG010000281         1977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFFG010000281 DAZFFG010000000
VERSION     DAZFFG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.283"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..940
                     /locus_tag="ACHJ7J_005059"
     CDS             <1..940
                     /locus_tag="ACHJ7J_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO5003137.1"
                     /translation="FVFLLPMIIFYQPWVNALPSTPRHASPEQLEKTVRYLTQTVHPR
                     SADNIDNLNRSAEYIKEVFVSSGARVTSQDVPITGGPYKNIVADYGPADGPLIIIGAH
                     YDSASSYENDQLTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPHFFR
                     SDEMGSAVHAASLEHPVKLMIALEMIGYYDSAPGSQNYPYPAMSWLYPDRGDFIAVVG
                     RMQDINAVRQVKAALLSSQDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAIMITDTAF
                     YRNKQYHLPGDTADRLNYQKMAQVVNGVITLLYNSK"
     gene            1043..1546
                     /locus_tag="ACHJ7J_005060"
     CDS             1043..1546
                     /locus_tag="ACHJ7J_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO5003138.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRNELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            1698..>1977
                     /locus_tag="ACHJ7J_005061"
     CDS             1698..>1977
                     /locus_tag="ACHJ7J_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFO5003139.1"
                     /translation="MKLNKALDYTFLVNKLKAQGIIFYAGLSEAEITAIEQTFNFCFP
                     LDFKAFLHTALPSTEGFIHWRQALHSSKMENEVKQKFKPPLEGVLYDVI"
BASE COUNT          565 a          390 c          460 g          562 t
ORIGIN      
        1 atttgtgttt ttactcccta tgattatttt ttaccaaccc tgggtcaatg cattaccgtc
       61 aacgccccga cacgcaagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac
      121 tgtgcatcca cgtagtgccg acaatattga taatctgaat aggtccgcag agtatataaa
      181 agaggtcttt gtcagtagcg gtgccagagt tacctcgcag gatgtcccca ttacgggtgg
      241 cccctacaaa aacattgttg ctgattatgg tcctgccgat ggaccgctga ttattattgg
      301 tgcgcattat gactctgcca gcagttatga aaacgatcaa ttgacctata cgccgggcgc
      361 ggatgataac gccagcggtg tggcaggatt actcgaactg gcacgtttgt tacatcagca
      421 agtaccgaaa acaggcgtgc agctggtcgc ctatgcgtcg gaagaaccgc acttctttcg
      481 tagcgatgaa atggggagcg cggtgcatgc agcttcgctt gagcatccag tgaaattaat
      541 gatagcactg gagatgattg gctattacga ctctgcgcct ggaagccaga attaccctta
      601 cccggcaatg tcctggcttt atcccgatcg gggagacttt attgccgtgg tcggcagaat
      661 gcaggatatc aacgccgttc gtcaggtaaa agcggcattg ttgtcatctc aggatttatc
      721 tgtttattct atgaatgccc cagggtttat tcccggtatt gatttctctg atcacctgaa
      781 ttattggcaa cacgatattc ccgccataat gattactgac accgcttttt atcgtaataa
      841 acaataccac ttgcccggtg ataccgcaga cagattgaat tatcagaaaa tggctcaggt
      901 agtgaatggt gttataactt tgttatacaa cagtaaataa taaaaatttc tcgaggtggg
      961 tccagaaaaa gcgacatgca cattttattt tccctcatct gttttaagat gcgaaacatg
     1021 aaattcaacg gaggaaaaat aaatgggaat gattggctat tttgcagaaa ttgactcaga
     1081 aaagattaac caactgcttg agtctactga aaaacctttg atggataata tacatgacac
     1141 gctttcagga ttacgccgac tggatattga taaacgctgg gattttttac actttgggct
     1201 gacaggcacc tctgcgttcg atccagcgaa gaatgatccg ctgagtcgtg ccgtgcttgg
     1261 tgaacacagc ctcgaagatg gtatagatgg tttcctggga ttgacatgga atcaggagtt
     1321 agccgcgaca atcgaccgac tggaatcact ggatcgaaat gaactgcgta aacagttttc
     1381 cattaagcga ctcaatgaaa tggagattta tccgggagtg accttttcag aagagttaga
     1441 ggggcaactc tttgcttcga tcatgcttga tatggaaaag ctgattagcg cgtatcgacg
     1501 tatgctgcga caagggaatc atgcgctgac ggttattgtt gggtgattta gttggcagca
     1561 gaattgaagg ttgatgtata gaagtttaaa tgaaacattc gttctaaaag ctggatgtga
     1621 tgcagaaatg ttttatccag cttacgaatg atttaacttc gttttagact gaagcataag
     1681 gatttaaggt tattgggatg aaattaaaca aggcattgga ctacacattt ctggttaaca
     1741 aattaaaagc gcaaggaatt atattctatg caggtttgag tgaggctgag attactgcca
     1801 ttgagcaaac ttttaatttt tgctttccac tcgattttaa agcatttttg catactgctt
     1861 tgccatcgac tgaaggtttt attcactggc ggcaagcgct gcactcatca aaaatggaaa
     1921 acgaggtgaa acagaaattc aaaccccctt tagaaggtgt attatacgat gttataa
//
LOCUS       DAZFFG010000282         1896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFFG010000282 DAZFFG010000000
VERSION     DAZFFG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.284"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(201..1613)
                     /gene="pykF"
                     /locus_tag="ACHJ7J_005062"
     CDS             complement(201..1613)
                     /gene="pykF"
                     /locus_tag="ACHJ7J_005062"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO5003140.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1621..1815)
                     /locus_tag="ACHJ7J_005063"
     CDS             complement(1621..1815)
                     /locus_tag="ACHJ7J_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003141.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          478 a          440 c          463 g          515 t
ORIGIN      
        1 tcgctcaaag aagcatcgaa cgctgggttt tagataaaaa aattgagaga aaacaggatg
       61 cttccatcgg attcatctta gataaagtga gattatatag tgaaatccga tttgattttt
      121 tattgcttct ggttatcgat taaataaaaa aagcgcccat cagggcgctt cgatatacaa
      181 attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt agtgccgctc
      241 ggtaccagtg caccagaaac cataactaca acgtcacctt tgtgtgccag accgctctgc
      301 agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc tttaaccagc
      361 tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt ggtggtcagt
      421 gccaggatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt accgccctgg
      481 gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc accacggcat
      541 accgcttcgg taatgcgcag tttacggttg tcattgttga actcaagacg gctgttcatc
      601 acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag cgggtattta
      661 ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat ggcgtttgca
      721 acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc cagcatctgg
      781 gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat cttctgggcg
      841 aagataactt cttctaccgg gatttctaca cccaggtcgc cacgcgcaac catgatgccg
      901 tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc gattttggag
      961 atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc gataacgtca
     1021 gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca accaaagatc
     1081 aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc aggcaggttc
     1141 acacctttgt tttcacccag gtcgccgttg ttcagcactt tacagataac tttgttacct
     1201 tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt gttgccaaca
     1261 gacaggtcag ttgtgaaacc ttcatacgtt accgcaacca tttcgctgtt gccgataaca
     1321 gatttatcag tagtgaaagt aaaggtctga ccagctttca gagaaacgtc gttaccgcct
     1381 tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc ggcggtttta
     1441 ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc tgcatagtca
     1501 ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt agctaacatc
     1561 tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt catgacagtc
     1621 ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact gccggaaagc
     1681 gaatccggta ttacgaaagt ggtggtgcct cgctctaagg ataggtgaca tcgaaaaagc
     1741 gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt tgagtcgttg
     1801 agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca ttatacgctg
     1861 aaattaatcc aaaaggaaaa caaaaacagc gtttca
//
LOCUS       DAZFFG010000283         1858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFFG010000283 DAZFFG010000000
VERSION     DAZFFG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.285"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            24..1157
                     /gene="ompN"
                     /locus_tag="ACHJ7J_005064"
     CDS             24..1157
                     /gene="ompN"
                     /locus_tag="ACHJ7J_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO5003142.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYIDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1298..1732
                     /gene="uspF"
                     /locus_tag="ACHJ7J_005065"
     CDS             1298..1732
                     /gene="uspF"
                     /locus_tag="ACHJ7J_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO5003143.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          500 a          433 c          424 g          501 t
ORIGIN      
        1 aaaataacta aaggatttat tcaatgaaaa gcaaagtact ggcactttta attcctgccc
       61 tgctcgccgc aggtgctgca catgcagccg aagtttataa taaagacggc aacaaattag
      121 atctgtatgg caaagttgat ggcctgcatt atttttctga taattcagcg aaagatggcg
      181 accagagcta tgcgcgtctg ggttttaaag gcgaaaccca aattaacgat caactcactg
      241 gctacggtca atgggaatac aatattcagg caaacaacac tgaatcttca aaaaaccagt
      301 catggacccg tctggcattt gccggactga aatttgcaga ttacggttct ttcgattacg
      361 gacgtaatta tggcgtaatg tacgacatcg aaggctggac cgatatgctg cctgaatttg
      421 gcggtgactc ttataccaat gcagacaact ttatgactgg tcgagccaat ggcgtcgcga
      481 cttatcgtaa tactgatttc ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc
      541 aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac caacaacgga cgtgatgttc
      601 gccatgaaaa cggtgacggc tggggtcttt ccacaacata tgatttaggc atgggcttta
      661 gcgctggtgc ggcatacacc tcttctgacc gcaccaatga ccaggttaac catactgcgg
      721 cgggtggtga taaagcagac gcgtggactg ctgggctaaa atacgatgct aacaatattt
      781 acctggcaac catgtattca gaaacgcgta atatgacccc gtttggcgac agcgattatg
      841 ctgttgcaaa caaaacccag aactttgaag tcactgcaca gtaccagttt gattttggcc
      901 tgcgtccggc agtctctttc ctgatgtcta aaggccgtga cctgcacgct gccggtggtg
      961 cagacaaccc ggcaggtgtt gatgataaag atctggttaa atacgccgat gttggcgcga
     1021 cttactattt caataaaaac atgtccacct atattgacta taaaatcaac ctgttggatg
     1081 aagatgacag tttctacgct gccaacggca tctctaccga tgatattgtc gctttaggtc
     1141 tggtttatca gttctaaatc ctcctgcccg ctgttatggc gggctttttc tgcttattct
     1201 tcctcctttg atctaaatta aaaatgtgaa ctccgtcatt acacaaaaag tgtcatctgg
     1261 cgttacactt tatgcggata ctaaaacagg aggttttatg aacagaacga ttcttgtccc
     1321 tatcgatatt tccgattcag aattaactca acgcgtgatt agccacgttg aggcagaggc
     1381 aaagattgat gatgcagagg ttcatttcct gacggtaata ccttcactgc cctactatgc
     1441 ctctctgggt ttagcgtatt ccgcagaatt accggcaatg gatgacctga aagcggaagc
     1501 caaatcgcaa ctggaagaga tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt
     1561 ccatgttgag gaaggttcgc ccaaagaccg cattctggaa ttggcgaaga agatccccgc
     1621 tcatatgatc atcattgctt cccatcgacc ggatatcacc acttatctgc tcggttccaa
     1681 cgccgcagct gtagtgcgtc atgcagagtg ctccgtgctg gttgtgcgct gacactaacg
     1741 cccgcacatt gctgcgggct ttttgattca tttcgcaaat gtgctgacat tttcccctct
     1801 aatccgtacc atacacgcca ctgtttttat atcagatttc ttatgctggg cattccgg
//
LOCUS       DAZFFG010000284         1736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFFG010000284 DAZFFG010000000
VERSION     DAZFFG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.286"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(117..560)
                     /locus_tag="ACHJ7J_005066"
     CDS             complement(117..560)
                     /locus_tag="ACHJ7J_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5003144.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            717..1646
                     /gene="metA"
                     /locus_tag="ACHJ7J_005067"
     CDS             717..1646
                     /gene="metA"
                     /locus_tag="ACHJ7J_005067"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO5003145.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          420 a          480 c          392 g          444 t
ORIGIN      
        1 aagcgagagg ccatcctgac ggatggcctt tttgcgttgg tgcaaaaaaa tgccggatgc
       61 gacgctggcg cgtcttatcc agcttacgca ggcacgatag cacgataggg ggcagcttat
      121 tcccccacat acgccagatt cagcaacgga tacggtttcc ccaaatcgtc cacctcagag
      181 cgtcccgtaa ccttaaaacc caccttctta tagaacccaa ccgcctgctc attttgctca
      241 ttaacgttgg ttgtcagttc cggggccatt gagagcgcat gcttcaccag catccggccc
      301 acgccgcagc cgcacacatc aggatcgata aacagcgcat ccatatgctg cccacttagc
      361 aacataaatc caaccggctg atcccgctca ttaaccgcga cccacaacgg cgcttccggc
      421 agaaaggaac gaaccagctc ttccagctcg gcccgatact ctgctgataa aaaatcgtgg
      481 gtggcatcga cagatcgaca ccaaatcgca acgagttcct ccccttcctc atgccgtgag
      541 cggcgaatac taataaccat tttctctcct tttagtcatt cttatattct aacgtagtct
      601 tttccttgaa actttctcac cttcaacatg caggctcgac attggcaaat tttctggtta
      661 tcttcagcta tctggatgtc taaacgtata agcgtatgta gtgaggtaat caggttatgc
      721 cgattcgtgt gccggacgag ctacccgccg tcaatttctt gcgtgaagaa aacgtctttg
      781 tgatgacaac ttctcgtgcg tctggtcagg aaattcgtcc acttaaggtt ctgatcctta
      841 acctgatgcc gaagaagatt gaaactgaaa atcagtttct gcgcctgctt tcaaactcac
      901 ctttgcaggt cgatattcag ctgttgcgca tcgattcccg tgaatcgcgc aacacgcccg
      961 cagagcatct gaacaacttc tactgtaact ttgaagatat tcaggaacaa aactttgacg
     1021 gtctgatagt taccggtgcg ccgctgggtc tggtggagtt taatgatgtc gcttactggc
     1081 cgcagatcaa acaggtgctg gagtggtcga aagatcacgt cacctcgacg ctgtttgtct
     1141 gttgggcggt ccaggccgcg ctcaatatcc tctacggcat tcctaagcaa acccgcaccg
     1201 acaaactctc tggcgtttac gagcatcaca ttctccatcc tcatgcgctt ctgacgcgtg
     1261 gctttgatga ttcattcctg gcaccgcatt cgcgctatgc tgactttccg gcagcgttga
     1321 ttcgtgatta taccgatttg gaaattctgg cagagacgga agaaggggat gcatatctgt
     1381 ttgccagtaa agataagcgc attgcctttg tgacgggcca tcccgaatat gatgcgcaaa
     1441 cgctggcgca ggaatttttc cgcgatgtgg aagccggact agacccggat gtaccgtata
     1501 actatttccc gcacaatgat ccgcaaaata caccgcgagc gagctggcgt agtcacggta
     1561 atttactgtt taccaactgg ctcaactatt acgtctacca gatcacgcca tacgatctac
     1621 ggcacatgaa tccaacgctg gattaatctt ctgtgatagt cgatcgttaa gcgattcagc
     1681 accttacctc aggcaccttc gggtgccttt tttatttccg aaacgtacct cagcag
//
LOCUS       DAZFFG010000285         1713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFFG010000285 DAZFFG010000000
VERSION     DAZFFG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.287"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(104..1129)
                     /locus_tag="ACHJ7J_005068"
     CDS             complement(104..1129)
                     /locus_tag="ACHJ7J_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFO5003146.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQNYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            complement(1155..>1713)
                     /gene="rfaY"
                     /locus_tag="ACHJ7J_005069"
     CDS             complement(1155..>1713)
                     /gene="rfaY"
                     /locus_tag="ACHJ7J_005069"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO5003147.1"
                     /translation="KVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVET
                     DRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKAS
                     MEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEI
                     KDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          538 a          348 c          282 g          545 t
ORIGIN      
        1 aagggtaatc attgctcatc gtgttctctt gcctgaacta ataaaatatg atagtactca
       61 tccttaatta ttattgtaac tcagacatcc atgattttta ttattatcca aatagttttc
      121 ttttatactt atttaattta aatttaatga agtaaataaa agcgttaaac gtatcaccat
      181 ttttctttgc atgttgatat ttcacttgat actgcttttc attcgtggct ggaataaacg
      241 ccacatcatt ccagcaagaa gcctgataag cctcattaaa atagttctgg cagggataat
      301 tcgtccagtc gttccagggc ttcgttacgc caacataatg aatcagctta gtatcatcgg
      361 taatggtctt tttataatct tcatcgcttt tattttttaa ctcataatca atgccataaa
      421 agcaatcata atcgccgcta ataaaaatac aatgaccaaa gaacagaata tttaaaatat
      481 cctgatcaag gcatttatac ttcgtgtgct gcgggtcact tgccagcata gcaatggctt
      541 tattcataaa atcatttttc gcccatgctt caagctgcag atatactaca ccggcgttga
      601 aataattccc tggaagtccc tctatagcca gacgcttcgc tggtttttcc tgcataaaag
      661 gcacatcttt aataacagct gcgaacttat ccccatcaaa attaatatca agcaatccag
      721 ctaatgagcc tttacagatg acatcagcat cgatatataa cagattttca atagtttcac
      781 tcaaatactg gaacgcaact aaacggaaga atgttgcata agaccactgt ttcaggctgg
      841 ggaagatatc aataaaggca ttggaaactt tataaattat tacttcaacg ttagcatttt
      901 tgctggtaaa agcattaaca cgttgtagat aatcttcatc aatgtagtcg gtaaagagat
      961 gaaacttaac cgcaaggtcc gggttatgca taacaactga ttgcatagat actgccgcac
     1021 caaataaaaa gttacggtca ataccgtaag cgatatttaa cacacgatcg gtgttagcaa
     1081 tttccccaga gacagcgact tcagtaatac tctcagctaa taaatccatg ttggttccgt
     1141 tttgactgtg tggtttacgc tttgcctttt aattttttaa taaatttgcg tagtttggtt
     1201 ctgtagatta ctgaatagta gccataatct tttatttcgt tggcaatacc caggtgacgt
     1261 tccatggcca gacgatcacg agctttacgc tctgccgtac agcttttacc ggataggtca
     1321 attatcctga caccatcttt actaacgata aagttacccc ggtgtgggtc gcccgatagc
     1381 atatttaagg catgcaattt ttccattgaa gccttaattt ctgctttaac attttctgga
     1441 ataatcggca tatcattgag ctctacgccc tcgacatact cgataagcat gataaaaaca
     1501 ctggcatagt tgaaaatttt acgctcagcc agaaaataaa aatcattagg aaaagtaaga
     1561 cctgcacttc tgacacgatc ggtttcaacg atcaaattct gatagtagtc accttttaca
     1621 aaagatttta aaaaacgttc attccttttc gttttcggag caaagacttt aaaaacatag
     1681 cgaccatatt tcgtatcaat caacgatact ttg
//
LOCUS       DAZFFG010000286         1706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFFG010000286 DAZFFG010000000
VERSION     DAZFFG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.288"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..628)
                     /locus_tag="ACHJ7J_005070"
     CDS             complement(<1..628)
                     /locus_tag="ACHJ7J_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO5003148.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGKSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTIRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWLYSLPGIIENNIISTEQRFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNRNYEILYGRDHVIYINTNSLDEAVW"
     gene            886..1386
                     /locus_tag="ACHJ7J_005071"
     CDS             886..1386
                     /locus_tag="ACHJ7J_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5003149.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRKLKPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            1388..1681
                     /locus_tag="ACHJ7J_005072"
     CDS             1388..1681
                     /locus_tag="ACHJ7J_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO5003150.1"
                     /translation="MSLVGYNSKIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLLISQDRLTH"
BASE COUNT          596 a          310 c          257 g          543 t
ORIGIN      
        1 cccagactgc ttcatccaaa ctattggtat taatatagat gacatgatct ctaccatata
       61 atatttcata attcctgttt ttgtttacaa gaattttatc gatatgtctc agctgcggat
      121 cattactggc attaacaata taaaaagcaa tgaaaatgat agcaaaaaga aaaaaaagca
      181 ttacatactt aaacagctta ctgctaaaga aacgttgttc tgtagagata atgttatttt
      241 caataatgcc tggcaatgaa tacagccaaa cttcattttt ttcttttaca gcaaatttga
      301 gacctaaaac ttctatttgc tgattcaatg ttaacgttct atcttcaggt tcttgtgctg
      361 ttaattctct gattgttaca accagatcag ggtcggtagt aattattatc tcaaaactac
      421 ctgtaagctc gccataagga acgactattg tattttctgg taactcagaa aaagcatcac
      481 tttttcctaa cgaaacatca ttaccaatga taacaagtag acgtccatta agtatttcat
      541 actcgcatcc attcaatggt ccgctcaaca aacgaatggc ataagattcc aataagtctt
      601 gtgataagaa tttatcatta ttttccatga ataccacccg ctaattccat aagctctagt
      661 ttagagtagt gtaacaagac ggaaatttca tgaccaaatt atgatgacac agaataaaaa
      721 aattataatt tcgagaacta ttttaaatgc ctctatgcat aactttattt acaatttaag
      781 gtataaaccc tattccctca atgttaagca aatataacaa ttactcttat tttaactttt
      841 acacgtaata tttaccctaa atgattttag agtacaggtt ggcatatgca agtcttttcc
      901 agtgatgtct acttcactgt aggcactaat gcattattag catcccaaaa agaatattac
      961 agcgatttag ttgcgttggt tgatcttggg cattcgttcg ttgttattga tgagcatcaa
     1021 catcgaaagc tcaaaccaaa tacagaacct gtaaacatcc ttttaagcaa caatttcatc
     1081 aggatcaata agaatattac gttatctgat ttaacgcatt tcctgatttc gaatcttcat
     1141 acacaaaatg tgtattccac acaagaacca cttacccatg atgagataga tatcctgcgg
     1201 ttatgtgttt cttactcgtt aaaacaaata gccattatta aaggaattga ttataaaaca
     1261 gtatcatatc acaaaataag agccctaaat aaacttaaca taaaaggtac tgttgagttg
     1321 tttatagcac tctgtgaatg ggataaacat tattttaaac tccaatcttg cgtaagagaa
     1381 agttgatatg tcattagtag gatataatag taaaatcata ataaccatcg cgctgatatc
     1441 gttgctctct gcatgcacca gagaaaattc attaaaggag aatcaaaata aaagaagtgg
     1501 cgaatcccaa gcgatagtag atgcaagaca gttctttagc gaacacccgg aatatatatc
     1561 ttcagcagaa ctggagcaaa agctatatcg cgaatttgca caggttacta cagttccagc
     1621 ttataaagag atgagtatgt atcaattgct tctcatttcg caagatcgtt taactcatta
     1681 aataatgaat ctacacgctt tttgcg
//
LOCUS       DAZFFG010000287         1702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFFG010000287 DAZFFG010000000
VERSION     DAZFFG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.289"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(116..226)
                     /locus_tag="ACHJ7J_005073"
     CDS             complement(116..226)
                     /locus_tag="ACHJ7J_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003151.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            complement(279..683)
                     /locus_tag="ACHJ7J_005074"
     CDS             complement(279..683)
                     /locus_tag="ACHJ7J_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO5003152.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(904..1635)
                     /gene="bluR"
                     /locus_tag="ACHJ7J_005075"
     CDS             complement(904..1635)
                     /gene="bluR"
                     /locus_tag="ACHJ7J_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO5003153.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLAAPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          468 a          362 c          359 g          513 t
ORIGIN      
        1 gatgatgact aaatcaaaac acatgtgcgt taagtattga aacgggcgta tggcacgccc
       61 gttgttttta taaatatatt aaccgttata aaataacgta tagaaagtca agtgatcaca
      121 tttcaaatat caatttgata gtattggcct ggtgattatt tatgggtagc aataaaagga
      181 cagtatttat catccatagg gatagtctct gtacttttat tcccattatg ctaatgcctt
      241 actgaattat gaagcatttc ttaagcatcc aactttagct agattaatgg tttattattt
      301 tctacatctt caatatataa aagcgtatta tcaatggcgt agtaactgcg tttgttatga
      361 ttaacatcag taacccaacg gaaaacgccc gcgtctgcca gtgttgaaca gtattcccga
      421 aatgtagatt ttccgcaaat atgaagcaat gcggcctctt ttattttagc agggttcttg
      481 gtcgtactaa cttttaacag gttcctggtt cctcttaata acaaaaccgt gtcatcgtga
      541 gtaataattc tgatgttatc cgtagccaga taataaatgt aatgtgcaat acggtgatgt
      601 tttaactctg aataaaacca ggagaagttt tgctcttttc tcacttgctc aaacatcttt
      661 tgaaaaacaa cgacctgatc catcaggata ataacctctt gttagttgtg agactgcgta
      721 gtgtgcacga tcggttttac cacttcaatc tggtctgtcc tttggctgtg atatgtacaa
      781 agtgtgaaag ggggattacc tgaattctcc cggtgagcat tttgtaatgg accagctccg
      841 gtacaaatgc tgttgtgggt tcagattata acattctgtc taaggggcgg gataaaggtg
      901 aaattagggg gcatgaaaga tgactttata accttgctca ccccagtgtt gtaaaagttc
      961 gttttgcctt ctcgttggtg ccatgcctgt ccagacaatc aatgtttgcg tcgggaacag
     1021 ttccgggcgc ggtgattcaa tgggagcagc aagaacagaa atatgccatc ctgacaatga
     1081 taaacgccat gcttccagcc acaatcgagc tctctcttca acattccacg ccatcagaat
     1141 ggcttcttta ccgggcttac ggcgcatttc gaaaagcgag gctgctgcgt attcaattaa
     1201 tgcgccgtca aacatactgc tcataatgcg ggaggtgttg tgatcaagca cgagacgctg
     1261 gcgaacagga aggtaaacat gattaatcaa ttgatctact gggtactctc gacccagtga
     1321 aataattctc gcgcgtagtt tggcaggatt agccatacga agaattgaca tcatctcttc
     1381 ttgcaggcgg ctccagtcat cttccgtatc ctggctggtg gtttccagta atgctttaac
     1441 tttgcctaca gggacgccat tacttatcca acgcttgatc tcttcgatac gttgtatgtc
     1501 ttcttcatca aagagtcggt gtccgccttc actgcgctgt ggttttaaca aaccgtagcg
     1561 gcgttgccag gcccggagag tgacaggatt aatcccgcaa cgttcagcaa catcaccaat
     1621 gctgtaataa gccacaattc ctccttgcgg tcataaatct ccgtcgcctg tatacgaccc
     1681 aataatactt tgtacaatat ac
//
LOCUS       DAZFFG010000288         1693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFFG010000288 DAZFFG010000000
VERSION     DAZFFG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.290"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..564
                     /locus_tag="ACHJ7J_005076"
     CDS             <1..564
                     /locus_tag="ACHJ7J_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO5003154.1"
                     /translation="LKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSA
                     MIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSY
                     PFNSPCPNGTEKVSAYHTHGADSHGEYWDKIFSGKDEKIVKSKDNNIKSFYLGTPSGN
                     FKAIDNHGKEITNGKGLPNVCRVHGNM"
     gene            536..918
                     /gene="yhhH"
                     /locus_tag="ACHJ7J_005077"
                     /pseudo
     CDS             536..918
                     /gene="yhhH"
                     /locus_tag="ACHJ7J_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
     gene            1023..1664
                     /locus_tag="ACHJ7J_005078"
                     /pseudo
     CDS             1023..1664
                     /locus_tag="ACHJ7J_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
BASE COUNT          619 a          244 c          360 g          470 t
ORIGIN      
        1 ctgaaggggg gatggaacct gtatggatat caattgaatc cgatatcaga catcgacccc
       61 ctgggtttat ctatgtggga ggatgcaaaa tcgggggcat gtactaatgg tctttgcggc
      121 acactatccg ctatgatagg tccagataaa tttgattcta tagatagcac cgcatatgac
      181 gccttaaata aaataaatag ccaatctatt tgcgaagata aagagttcgc tggtttaata
      241 tgtaaggata atagtggcag atatttctca acagcaccta accgaggaga aagaaaagga
      301 tcatatccat tcaatagccc ttgccctaat ggtactgaga aagtatcagc ttatcatact
      361 catggtgcag atagtcatgg agaatattgg gacaaaatat tttcaggtaa agatgagaaa
      421 atagttaaaa gtaaagataa caatatcaag tcattttatt taggtacgcc cagtggtaat
      481 tttaaagcaa tagataacca cgggaaggaa ataacaaacg gaaaaggatt acctaatgtc
      541 tgcagagttc atggtaatat gtaaaaaata ttgtttagga actgtgtcat tgtatctttg
      601 tttgttttta catacaacac atgggcgcag tgtaataata atattaaaat aatgcgcaag
      661 tatgaaagtg aaggtaaata taccgttaga aatttggtta aaaataaagc tatagcattg
      721 gaattagctg agatatatgt taagaatcgt tatggacagg atgccgcaga agaagaaaaa
      781 ccatacgaaa tcactgagtt aacaacaagt tgggttgttg aaggtaccat tcactcagac
      841 caaattgctg gtggggtttt tattatagaa ataggcaaaa atgatgggag aattctgaat
      901 tttggccacg gaaaataagt atgtaaggat aatcaaacat cgtgtgcgct gatggcagag
      961 taggtggagg actccagaca gtcaaacgat aaaaaaagat agcctttgtg gaggttcctg
     1021 caatgtcaaa tacataccag aaaagaaagg caagtaaaga gtatggttta tataatcaat
     1081 gtaagaaact aaatgatgat gaattatttc gcttacttga tgatcacaat tccttgaaaa
     1141 ggatttcatc tgccagagta ttacagttaa gaggtgggca agacgctgtt agattggcaa
     1201 ttgagttctg ctctgataaa aattatatcc gtagagatat cggagcattt atactcgggc
     1261 aaataaaaat ttgcaaaaaa tgcgaagata atatttttaa tattttgaac aatatggcat
     1321 tgaatgataa gagcgcttgc gttcgagcta cggcaatcga gtcaacggct cagcgatgca
     1381 agaaaaaccc aaattattaa cctaaaatag tagaacaatc tcaaattact gcttttgata
     1441 aatcgactaa tgtcagacgt gctacagcat ttgctatttc tgttatcaat gataaagcaa
     1501 caattccact attgattaat ctgttaaaag atccaaatgg agatgtcaga aactgggccg
     1561 catttgcaat aaatatcaat aaatatgata atagtgatat tagggattat tttgtggaga
     1621 tgcttcagga taaaaatgag gaagtccgta ttgaagcaat aatctgactt tcctacagaa
     1681 aagataaaag ggt
//
LOCUS       DAZFFG010000289         1634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFFG010000289 DAZFFG010000000
VERSION     DAZFFG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.291"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..357
                     /locus_tag="ACHJ7J_005079"
     CDS             <1..357
                     /locus_tag="ACHJ7J_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5003155.1"
                     /translation="FRLGSHNDMTFDEGTSSRDTLRGCAKHSVRELPVNWWVQPSVIR
                     TFSSRGNMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSG
                     SSAEGYNGQATLNVTF"
     gene            478..>1634
                     /locus_tag="ACHJ7J_005080"
     CDS             478..>1634
                     /locus_tag="ACHJ7J_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HFO5003156.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANG"
BASE COUNT          382 a          430 c          422 g          400 t
ORIGIN      
        1 ttccgtctgg gcagccacaa cgatatgacc tttgacgaag gcacctcatc ccgtgacacc
       61 ctgcgcggct gtgccaaaca cagtgtgcgt gaactgccgg tgaactggtg ggtacagcct
      121 tctgttatcc gcaccttcag ttcccggggg aacatgagca tgggtacagc cgcagccggc
      181 agtaacatga cgttctcacc gtcacagaat ggtacgtcac tggacctgca ggccggactg
      241 gaagcccgtg tccgggaaaa catcacccta ggcgttcagg ctggttatgt ccacagcgtc
      301 agcggcagca gcgctgaagg ctataacggt caggccacgc tgaatgtgac cttctgacag
      361 aaccatcgcc tctctgtggt ccctgtcatc atgatcggga cccggaccgg cgcaacggat
      421 attcaacgcc acattcgctg gcattcacaa taaaatgata ttcatcacgg agtgactatg
      481 ttacagatag tcggcgcgct gatcctgctg atcgcaggat ttgccattct tcgccttttg
      541 ttcagagcat taatcagcac ggcttctgcg ctggcagggc tcatattgct gtgtctgttc
      601 ggcccggcct tactggctgg ctatatcacc gaacgcataa cccggttgtt ccatattcgc
      661 tggctggcag gcgtatttct gacgattgcc ggaatgatca tcagcttcat gtggggactt
      721 gatggtaaac atatcgcgct ggaggctcac acctttgact ctgtgaaatt tattctgacc
      781 accgctctcg ccggtggtct gctggctgtt cccctgcaga tcaaaaacat tcagcagaac
      841 gggatcacac cagaagatat cagcaaggaa attaacgggt attactgctg tttttatact
      901 gcctttttcc ttatggcgtg ttctgcatgc gcaccattga tcgcgttaca gtacgatatt
      961 tcaccgtcac tgatgtggtg gggcgggttg ttgtactggc tggctgcatt agtgacgctg
     1021 ctatgggcgg ccagccaaat ccaggcgctg aaaaaactga cctgtgccat cagccagaca
     1081 ctggaagaac aaccggtgct caacagtaaa tcgtggctga ccagtttgca aaacgattac
     1141 agccttcctg actcactgac ggagcgcatc tggctgacgc tcatttctca acggatttcc
     1201 cggggagagc tgagggaatt tgaactggca gacggaaact ggttactgaa caatgcctgg
     1261 tatgaaagaa acatggcagg gtttaacgaa cagttgaaag agaacctgtc attcacacct
     1321 gatgaactga aaacgctctt ccggaaccgc ctgaatttat caccggaagc gaatgacgat
     1381 tttctcgatc gttgcctgga cggcggtgac tggtatccct tttcagaagg tcgccgtttt
     1441 gtatcattcc atcacgtgga tgagcttcgt atctgtgcct cctgcgggct gacagaagta
     1501 catcatgccc cggaaaatca taagccggat ccagaatggt actgctcctc tctttgtcgc
     1561 gaaacagaaa cactgtgtca ggaaatttat gaacgccctt acaacagctt tatttccgat
     1621 gcaacggcga atgg
//
LOCUS       DAZFFG010000290         1628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFFG010000290 DAZFFG010000000
VERSION     DAZFFG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.292"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(165..>1628)
                     /locus_tag="ACHJ7J_005081"
     CDS             complement(165..>1628)
                     /locus_tag="ACHJ7J_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFO5003157.1"
                     /translation="GMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNLANLSRDP
                     VHANDSISPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAQDKYGPVPA
                     DATEEQRQAYLAKLRDTPEYKKEQEKYGTGSDMQRGIQAATAALQGLAGGNMAGALAG
                     ASAPELAHLLKSTEKDPAVNAIAHAILGGAVAAMQGNNVAAGAAGAATGELAARAIAG
                     MLYPGVKQSDLSEEQKQTISTLATVSAGLTGGLTGNSTASAAVGAQSGKNAVENNALS
                     LPSGMVSYGQAVSSWNQYADANNLTPEQKQAGLDKIAKGELPEGANISKVIVDGYKDG
                     VLIAGAWYLGPAASVGKVIGGGVIAEIANGTYQWFDLSQPGNENKNWDWKSSASAGIT
                     GMLAPGRTVGQNVGIAMGSAFFTDGPNAGAIGGAAAGAWAGGLFGEYAPGIVNSVTGK
                     EIPGFVYDYWGGVASEFSSGFIKDLNKPKGSSEDKKK"
BASE COUNT          367 a          499 c          320 g          442 t
ORIGIN      
        1 tataaaacca aaaacgatag ctcttttaaa ctgattcact ggaaaataaa acccatcacc
       61 tttaaaaaaa taaaagattg aaaccactcc tcccagaaat agtaacatga ctatagatga
      121 taaacagcta taaaggaaca ataccacata actatatagt tgtttcattt tttcttatcc
      181 tctgacgaac ctttcggttt attgagatct ttaataaatc cagatgaaaa ctctgaagca
      241 acccctcccc aataatcata aacaaaccca ggaatttcct ttccagtaac agagtttaca
      301 attccaggtg catattcccc aaataatcca ccagcccatg cacctgcggc agcacctcca
      361 atagctcctg cattcggtcc atcagtaaaa aatgcactgc ccatcgcaat tcctacgttt
      421 tgtccaacag ttcttcccgg tgcaagcata ccagtaatgc ctgcactggc actgcttttc
      481 cagtcccagt ttttgttctc attacccggc tgactcagat caaaccactg gtatgttccg
      541 ttagctatct cagcaataac tccaccaccg atcactttcc cgacggacgc tgcaggcccc
      601 agataccagg cacctgcaat caatacgcca tctttataac catcaactat aactttgctt
      661 atattggctc cttcaggtaa ctcacctttg gcaattttat ccagtcctgc ctgcttctgt
      721 tccggggtga gattattggc atcagcatac tgattccatg aggaaaccgc ctgaccataa
      781 ctcaccattc ctgatggcag actgagcgca ttattctcaa cagcattctt cccgctttgt
      841 gctcccactg ctgctgatgc ggtgctgttt ccggttagac cgccggtcag ccctgctgaa
      901 acggttgcca gcgtacttat cgtctgcttc tgctcttcac tgaggtcaga ctgcttcacg
      961 cccggataca gcattccggc aatcgctctg gctgccagct ctcccgttgc tgcgcctgct
     1021 gcaccggcag caacattatt accctgcatt gccgctactg caccgcccag aatggcgtga
     1081 gcaatggcgt ttactgccgg gtctttttct gttgatttca gcagatgcgc cagttccggc
     1141 gctgaagcac ctgccagcgc gcctgccata ttgccgcccg ccaggccctg aagtgcagcc
     1201 gttgcagcct ggataccgcg ctgcatatcg ctgccggtac catacttttc ctgttccttt
     1261 ttgtattccg gcgtatcacg cagttttgcc agatatgcct gccgctgttc ttccgtcgca
     1321 tccgccggaa caggcccata tttatcctgc gcagccttca acgcattcag ttccccctgc
     1381 gtccgcgcga tatccgccac ctgactgcct atgtcactga taagccccac tgtctgcaga
     1441 cgccgttgct ctttctcctt gtcaaatatc gggctgatac tgtcgttagc gtgcacaggg
     1501 tcacggctca ggttcgccag attctgcttc tgattgtccc tgtcccggat ggtgatagtg
     1561 ccttctgcca ctgcggcctg agtcgttcct tccgcatgtc cgctgtgacc tccggcggat
     1621 atcatgcc
//
LOCUS       DAZFFG010000291         1606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFFG010000291 DAZFFG010000000
VERSION     DAZFFG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.293"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..568
                     /locus_tag="ACHJ7J_005082"
     CDS             <1..568
                     /locus_tag="ACHJ7J_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO5003158.1"
                     /translation="LNNYSDLKETTSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPV
                     PKPSTQVGQKVLDKILNYTNTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGL
                     QFYEVVPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNE
                     STPMAFENIKQVIDDMATDATMFDINKK"
     gene            complement(664..1004)
                     /gene="tnpA"
                     /locus_tag="ACHJ7J_005083"
                     /pseudo
     CDS             complement(664..1004)
                     /gene="tnpA"
                     /locus_tag="ACHJ7J_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1012..>1606
                     /locus_tag="ACHJ7J_005084"
     CDS             1012..>1606
                     /locus_tag="ACHJ7J_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5003159.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSNGTVF"
BASE COUNT          445 a          363 c          395 g          403 t
ORIGIN      
        1 tttaaacaat tactctgatt taaaagaaac aacctcggct acaggtaaac cggttttacg
       61 ttgggtagac ccgagttttg atcaaagcaa atatgacagc atcgtctgga acccaatcac
      121 ttattatccg gtaccgaaac cttcaaccca ggtagggcag aaagttctgg ataaaatttt
      181 gaactatacc aacaccgaaa tgaaagaagc gatagcgcag cgtaaaccac tggttaccac
      241 cgctgggccg cgtagtctga ttttccgtgg ggccattacc ggtgtagata ccagcaaaga
      301 agggctgcaa ttctatgaag tggttcctgt tgcattagtg gttgcgggga cgcaaatggc
      361 tacaggccac cgtaccatgg atactcgctt gtattttgaa ggtgagctga ttgatgcagc
      421 gactaataaa ccggttatca aagtcgttcg tcagggcgaa ggtaaagacc tgaataacga
      481 aagtacgcca atggctttcg aaaatattaa acaagttatt gatgacatgg cgaccgatgc
      541 caccatgttt gacattaaca aaaagtagtt ccagacgcgc catcgttaga tggcgctttt
      601 tatccggtgc gccgtaaaac cccatccttc agggcgggga tataaggcgc ggttttccac
      661 ctgaccaggt gtttgctgtt gttcaatgta ttggcggatg acggatattg gcgcagtaac
      721 ctacccgata cgcctttgag gctgtttacc agactggata tcgccagttt gggaggttag
      781 ttaattaaca aatggacgtg atctggttcg ccatccattt caaccagttc agcttcaaaa
      841 taagcacata catttgaaaa gtaagtgcgt agtttttctg tcgcgtcatg gtcaaaaatc
      901 tggcgtcggt atctggtgac aaagaccagg tgaacatgca tcaggaaaac acaatgcctt
      961 ccacgccgga tatcggtttc ttttttcaca gaccaaagta tcatttgacc tgtgaaacga
     1021 ttacaggcat ttaaattcca gttaagacca ggtggtcaac aggagtgtga aatgaggcgc
     1081 ttcgcaggcg catgtcgttt cgttttcaat cgtgctctgg cacgtcagaa tgagaatcat
     1141 gaggccggta ataaatacat cccttacggg aaaatggctt cctggctggt tgagtggaaa
     1201 aatgccactg aaacgcaatg gcttaaagat tctccctcac agccattgca acagtcactt
     1261 aaagaccttg agcgggctta caaaaacttc ttccggaagc gggctgcttt tccccgattc
     1321 aaaaaacggg gacagaatga tgcattccgc tacccgcagg gcgttaagct cgatcaggaa
     1381 aacagccgta tttttctgcc gaaactgggc tggatgcgct accggaatag ccgtcaggtc
     1441 acgggtgttg tgaaaaatgt cactgtcagc cagtcctgcg gtaagtggta catcagtatt
     1501 cagacagaaa gtgaagtatc cactccggct cacccttcag catcaatggt cgggctggat
     1561 gctggcgtgg ctaaactcgc cacgctgtca aatggcacag tctttg
//
LOCUS       DAZFFG010000292         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFFG010000292 DAZFFG010000000
VERSION     DAZFFG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.294"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            803..1405
                     /gene="fimB"
                     /locus_tag="ACHJ7J_005085"
     CDS             803..1405
                     /gene="fimB"
                     /locus_tag="ACHJ7J_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO5003160.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          488 a          273 c          331 g          495 t
ORIGIN      
        1 cttggtgaaa ataagtgttt acccgccatc aggctgagca taattctcat catgaaatat
       61 gtttcctggt ttgtggcttg taactggtca cttctgaagt cgatctggag aggcttgttg
      121 atgttggtgt tttcaggatg atgtttcact tagtttgttt gccgtatcgc ccggcgaatg
      181 gctgtgattg aggaaggttt aagtcgcagt gaccaaagcc atatttacca gcgaatgtag
      241 atgaaaaaat cgtctcctgc gttcccccat atctccagga taaaaaggaa tgtaacaatc
      301 tcatggctta aactgacgaa ccagccagga ataatcgcta gggacctaag aattagcttg
      361 ataatagcca ctaagaaatt actgtgttcc atgaaatagc cattttgtgg caaatggagt
      421 tgactaataa tgtcatatgt gagacggcta gttgaacgaa tattaaattt tgctgaattt
      481 tttatgttga ttttacttgt tacagaacat atcacatgat atatagataa gattagttgc
      541 attaatgatg agggttatta ttagattcgt atcagattga taaatatata aaagtatata
      601 gcatgcaaga gcatagcgtt tgtatggcaa cgttattata attaactgtt gctaccccat
      661 ttaagttcac tcagaagaac tggtccactt acgttagtta ttaagcaaac gttcgctttt
      721 ataaacataa tcaggataaa aatgttggat tattgctaac ccagcacagc tagtgcgcgt
      781 ctgtaattat aagggaaaaa cgatgaagaa taaggctgat aacaaaaaaa ggaacttcct
      841 gacccatagt gaaatcgaat cactccttaa agcagcaaat accgggcctc atgcagcacg
      901 taattattgt ctgactttgc tttgttttat tcatggtttc cgggcgagtg aaatttgtcg
      961 attgaggatt tcggatattg atcttaaggc aaagtgtata tatatccatc gattaaaaaa
     1021 aggcttttca acaacgcacc cgctattgaa caaagaagtt caggctttaa aaaactggtt
     1081 gagtatccgt acttcgtacc cgcatgctga gagcgagtgg gtatttttat cgcgtaaggg
     1141 gaatccgctt tctcggcaac agttttacca tattatctcg acttccggtg gtaatgccgg
     1201 gttgtcactg gagattcatc cgcacatgtt acgccattcg tgtggttttg ctttggcgaa
     1261 tatgggaata gatacgcgac ttatccagga ttatcttggg catcgcaata ttcgtcatac
     1321 tgtctggtat accgccagca atgcagggcg tttttacggc atctgggata gagccagagg
     1381 acgacagcgt cacgctgttt tatagagata gttgagatac cagggatggt gttaaaaact
     1441 acattattct tacgatatat caaaaatgat taaccattta ttaattgatg ttattgtaag
     1501 tataattctt aattatcata ataaacatta agttaaccat atccatacaa aatacaatgg
     1561 tttatgttct tcaaaataaa taaacaa
//
LOCUS       DAZFFG010000293         1556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFFG010000293 DAZFFG010000000
VERSION     DAZFFG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.295"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            1..556
                     /locus_tag="ACHJ7J_005086"
                     /pseudo
     CDS             1..556
                     /locus_tag="ACHJ7J_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            838..1158
                     /locus_tag="ACHJ7J_005087"
     CDS             838..1158
                     /locus_tag="ACHJ7J_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003161.1"
                     /translation="MDIWPEFQRDLEMYRDVVLSIKRNLRLYEECIESLVHQIGSTNF
                     DNAQPLFDDLFRMQSELATVLYKYEYKPGKRIQDLIYHLDRDDFYSRKYWHKKFSDGL
                     AWPE"
     gene            1282..>1556
                     /locus_tag="ACHJ7J_005088"
     CDS             1282..>1556
                     /locus_tag="ACHJ7J_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFO5003162.1"
                     /translation="MPARQVCQNFFHDALAPFHKYRQNALLDATIALINGASLTLTSI
                     ERYLPGLARVKNKIKRVDRLLGNESLHRDIPRIFSNIIAMLTSQLSR"
BASE COUNT          478 a          319 c          379 g          380 t
ORIGIN      
        1 ggcatgatat ccgccggagg tcacagcgga catgcggaag gaacgactca ggccgcagtg
       61 gcagatggca caatcaccat ccgcggcggg gacaatcaga agcagaatct ggcgaatctg
      121 agccgtgacc cggaacacgc caacgacagt atcagcccga tatttgacaa ggagaaagag
      181 cagaggcgtc tgcagacagt ggggcttatc agtgacatag gcagtcaggt ggcggatatc
      241 gcgcggacgc agggggaact gaatgcgttg aaggctgcgc aggataaata tgggccttgg
      301 cccttataac tcgaaataca gccagcaata cgctgatgcg tatggtcaga tagtcaatct
      361 cctcgatatc accagtgttg atgcacagaa tcagcagcag gtgaaaaacg ccatgattaa
      421 ctatttcatg gtcaccaaag gggtggatcg tcagaccgca gaaagctaca ccgaaacaaa
      481 gcagggcttg gagatcattg cggcttcggt aacgcctctt atagggcaag ctgcatcaaa
      541 taaattaagc taccttggta ttgggaaaaa gatatccttt gatggtgatt tttatactgt
      601 agatggaatg aaattcagca aaagttatta tgagaaatta tgggcgcaag gcaggacctg
      661 cgccattcgt gcaatcaaga gaagttttga atagcaatcc aaaaattgag ccggatccta
      721 gaggcgcacc tggctactta agatatgaag gtgcaggtct tgaaatgatc tataacccga
      781 aaacgggaca agttggtcat attcaaccag tcaaggttaa ataaaaagag gaagtaaatg
      841 gatatttggc ctgaatttca acgagatctt gagatgtatc gagatgttgt tttatcaata
      901 aaacgtaacc ttcggttgta tgaggagtgt attgaatcgt tagttcatca gatcggaagc
      961 accaattttg ataatgcaca gcctctattt gatgacctct ttcgtatgca atctgaactt
     1021 gcaactgtgt tatacaaata tgaatataag ccaggcaaaa ggatacaaga tttaatatat
     1081 catctggaca gggatgattt ttactcaaga aaatattggc ataaaaaatt cagtgatgga
     1141 ttggcatggc ctgaataata gaagtagaaa tcccgccctc agttagcgct gaggaatcct
     1201 tagtaatggg cgggtaaaaa agacaggcat agagttttgt gatctactga tttgtgcaac
     1261 caattcaatg agatcgcttc tatgcctgct cgacaagtat gtcagaattt tttccacgat
     1321 gcgttagcac cgtttcacaa ataccgacaa aatgctttgc tggatgccac catcgctctc
     1381 atcaatggcg catcactgac gctgaccagc atcgagcgtt atttacccgg cttggctcgg
     1441 gtcaaaaata aaatcaaacg cgttgaccga ctgctcggta atgaatctct tcaccgcgat
     1501 atcccacgga ttttcagtaa tattattgcc atgctgacta gccagctatc tcgtaa
//
LOCUS       DAZFFG010000294         1502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFFG010000294 DAZFFG010000000
VERSION     DAZFFG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.296"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>1502)
                     /locus_tag="ACHJ7J_005089"
     CDS             complement(<1..>1502)
                     /locus_tag="ACHJ7J_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5003163.1"
                     /translation="DGTVEISITSQTAGISTVTATINNSTLSQNVTFVADVRTAKIAD
                     LEVIKDGSVADGSTANTLRVRVTDAFGNALAGQTVSVLADNGATVSPTVITGPDGTVE
                     ISVTSQTAGTSVVTASINNSSLSRDVMFIADIRTAQIADLVVIKDGSEADGSTANTLR
                     ARVTDAFGNALAGQTVSVLADNGATVAPTVTTQPDGTVEISVTSQTAGTSTVTASINS
                     SSLSRNVTFIADVRTAKIADLVVIKDGSEADGSTANTLRARVTDAFGNELAGQTVSVL
                     ADNGATVASTVTTGQDGTVEISVTSQTAGISAVTVSINNSTLSQNVMFIADIRTAQIA
                     ELVVIKDGSEADGATANTLQVKVTDAFGNTLAGQTVSVMADNGAAVASMMTTKPDGTV
                     EISVTSQTAGISVVTASINSSSQSRDVTFVADIRTAQIADLVVIKDGSVADGAMANKL
                     RARVTDAFGNALAGQTVSVFAGNGATTAPTVTTQPDGTVEISVTSQTAGT"
BASE COUNT          245 a          523 c          404 g          330 t
ORIGIN      
        1 ggtgcctgcc gtctggctgg tgacagagat ctccaccgtg ccgtccggct gcgtggtgac
       61 cgtcggagca gtcgtggcac cgttgcctgc gaacacgctg accgtctgcc cggcaagtgc
      121 attaccgaac gcatcggtga ccctcgcccg cagcttattc gccattgcgc cgtcagccac
      181 tgagccgtcc ttaatgacca ccaggtccgc gatctgcgcc gtcctgatat cggcaacaaa
      241 cgtcacatcc cggctctgac tgctgctgtt gatgctggcg gtgaccacac tgattcctgc
      301 tgtctggctg gtgacactga tctccaccgt gccgtccggc tttgtggtca tcatcgaagc
      361 gaccgctgca ccgttgtccg ccatcacgct gaccgtctgc ccggcaagcg tattaccgaa
      421 cgcatcggtg accttcacct gcagcgtgtt cgccgtcgca ccgtccgcct ctgaaccatc
      481 cttaataacc accagctccg cgatctgcgc cgtcctgata tcggcgataa acatcacgtt
      541 ctggctcagg gtgctgttgt tgatgctgac ggtgaccgca ctgattccgg cggtctgact
      601 ggtgacactg atctccaccg tgccgtcctg ccctgtggtg accgtcgaag cgaccgttgc
      661 gccgttatcc gccaacacgc tgaccgtctg cccggcaagc tcattaccga atgcatcggt
      721 gaccctcgca cgcagcgtgt tcgccgtcga accgtccgcc tctgaaccat ccttaatgac
      781 caccagatcc gcgatcttcg ccgtcctgac atcggcgata aacgtcacgt tccggctcag
      841 gctgctgctg ttgatgctgg cggtgactgt actggtaccc gccgtctggc tggtgacact
      901 gatctccacc gtaccgtccg gctgagtggt gaccgtcggg gcaaccgttg cgccgttgtc
      961 cgccaacacg ctgaccgtct gcccggcaag cgcattgccg aacgcatcgg tcaccctcgc
     1021 ccgcagcgtg ttcgccgtcg aaccgtccgc ctctgaacca tccttaatga ccaccagatc
     1081 cgcgatctgc gccgtcctga tatcggcgat aaacatcacg tcccggctca ggctgctgtt
     1141 gttgatgctg gcagtgacca cactggtgcc cgccgtctgg ctggtgacag agatctccac
     1201 cgtgccgtcc gggcctgtga tgaccgtcgg agacaccgtt gcgccgttgt ccgccaacac
     1261 gctgaccgtc tgcccggcaa gtgcattacc gaacgcatcc gtcaccctca cccgcagcgt
     1321 gttcgccgtc gaaccgtctg ccactgagcc gtccttaatg acctccagat cagcgatctt
     1381 cgccgtcctg acatccgcga caaatgtcac gttctgactc agggtgctgt tgttgatggt
     1441 tgcagtgact gtactgatcc ccgccgtctg gctggtgata ctgatctcca ccgtgccatc
     1501 cg
//
LOCUS       DAZFFG010000295         1492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFFG010000295 DAZFFG010000000
VERSION     DAZFFG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.297"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(85..441)
                     /locus_tag="ACHJ7J_005090"
     CDS             complement(85..441)
                     /locus_tag="ACHJ7J_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003164.1"
                     /translation="MINLSLIDLNRIQFREKFTWQLLVNVENGRVVSNYHLPDGAIAG
                     SVEALLELAERARLIKPLTCHHDDDLHFTGRMMSNYENGVEASRERLHDDYCFGTLPE
                     FIELLTSCGYQVIQGG"
     gene            complement(434..622)
                     /locus_tag="ACHJ7J_005091"
     CDS             complement(434..622)
                     /locus_tag="ACHJ7J_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5003165.1"
                     /translation="MATTPTLIHSQTAFIWRFIAFGASESQIINVTAWTEREARNHCP
                     SGCVAVFAARIRQEVCHD"
     gene            complement(656..1114)
                     /locus_tag="ACHJ7J_005092"
                     /pseudo
     CDS             complement(656..1114)
                     /locus_tag="ACHJ7J_005092"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            1113..1307
                     /locus_tag="ACHJ7J_005093"
     CDS             1113..1307
                     /locus_tag="ACHJ7J_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HFO5003166.1"
                     /translation="MSTVIKRDKKQKGTGKAPPFHMRIVPELKEQFDNEASNDGVSLA
                     NWLKELGRAELKRRGIEPKG"
     gene            complement(1340..1474)
                     /locus_tag="ACHJ7J_005094"
                     /pseudo
     CDS             complement(1340..1474)
                     /locus_tag="ACHJ7J_005094"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
BASE COUNT          376 a          370 c          390 g          356 t
ORIGIN      
        1 atattgataa aagcgcatca cccgcatcat aagacacgcc ggaaaattcc tgtttcaggg
       61 aattaaaacg atcatcacgc atgtttaccc cccctgaatg acctgataac cgcaactggt
      121 cagcaattcg ataaattccg gcaatgtgcc gaaacagtaa tcatcatgca gacgttcgcg
      181 ggatgcttca acgccgtttt cataattgct catcatacgt ccggtaaaat gcagatcatc
      241 atcgtgatgg cacgttaacg gcttaatcag tcgcgcacgt tctgccagct ccagcaatgc
      301 ttcaacgctt ccggcaattg caccatccgg caggtgataa ttacttacca cgcgtccatt
      361 ctctacattg accagtagct gccaggtaaa tttctcacga aactggatgc ggttaaggtc
      421 gataagtgac aggttaatca tggcacacct cctgacgaat acgggcggcg aatacagcga
      481 cacaaccgga cgggcaatgg ttacgcgctt cgcgttctgt ccaggcggtt acgttgatga
      541 tttgggattc tgacgcgcca aaggcaataa agcgccagat aaaggcagtt tgagaatgta
      601 taagggtagg ggtagtagcc atgttggcag cctccttaga cgttgggata tccccaccgc
      661 cggaggtggt aagctcgctg gcggtggact gtacagggtt accacaactg gcgtccaagg
      721 tgaccagccc gaccgaagtc ggccccgtac agcccaccat tgttaaggtg tgcgtgtgcg
      781 ccgatacaaa aaaagacgcg tgcggcgtct gtatcgccct ggacatcagc ggggtggtaa
      841 tcccggcacc cgttttatga ggtgcacatg aatcatagcc ctggctggca gtggcgatca
      901 acaggttttt catacccgct ttattcaggt tgtgcgagtc ccgcccttgc gggtgtgtgg
      961 tatgatttaa catagctacc tcgataatct tgctatcgtt ggtggttaga cgccctgcat
     1021 gtgtgccacc actgcggggc gttgcgtttt ttgatgtaca cgtgttaagg tgtacaccta
     1081 tcaaaccaaa acataacgct ataggtgtac acatgtcaac agttattaag cgcgataaaa
     1141 aacaaaaagg cacaggaaag gccccaccat tccatatgcg tatagtccct gaattgaagg
     1201 agcagtttga taacgaagcc agtaacgatg gagtaagcct agccaactgg cttaaagagt
     1261 tggggcgcgc tgaactaaag cggcgcggta ttgaacccaa aggctgacaa tccaccagca
     1321 agccagttgg ggattgctgg ctggcttcct gttcgctggt ggcctcgatg gtaatcacgc
     1381 gcggttgtgc tgtactcagg gcgataaaac gccagatgta tttattcagg ttgtgcgagt
     1441 cccgcccttg cgggtgtgtg gtatgattta acatagctac ctcgatactc tt
//
LOCUS       DAZFFG010000296         1489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFFG010000296 DAZFFG010000000
VERSION     DAZFFG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.299"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1326
                     /gene="espX1"
                     /locus_tag="ACHJ7J_005095"
     CDS             <1..1326
                     /gene="espX1"
                     /locus_tag="ACHJ7J_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO5003167.1"
                     /translation="KVFCEYKTEFLILFDDFFRSQDLPKPSPVLHHFFQYTHLRDAHF
                     YRCKLIEHTVQFYFFKHKGITLLRLDVFDDRTSECLSEEIKIYQECYEKFVKFLKANF
                     NQEIYPQLYTPEIFYEACRNLQSFYAHQETSQNAKYSAIDKKKSHLNQEIKKLIKKNI
                     FPELYNEQCNKIPSSSTDDNQQITWQNFKTSNSAYSQLCEKLSLFKSVPSRLIEKSAY
                     CSTENMITNKFDVVFSFCGDNIKEFILLLPYNKSLEMYELNEQKIQYVTTPNININKL
                     LLSNITIKKSNLSHGYYFGCVLSNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFT
                     NTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLV
                     RDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFLMKKISHQD"
BASE COUNT          568 a          263 c          219 g          439 t
ORIGIN      
        1 aaagtgtttt gcgagtataa aacagagttc ttaattttgt ttgatgactt tttcagaagt
       61 caggatttac ctaaaccatc tccagtcctt catcattttt ttcaatatac tcatctgaga
      121 gatgcacatt tttatagatg taaactcata gaacatacag ttcaatttta tttttttaaa
      181 cacaaaggca tcacattact tagattggat gtttttgatg acagaacaag cgaatgtctt
      241 tctgaagaga ttaagatata tcaagaatgc tatgaaaaat tcgttaagtt tttaaaggca
      301 aacttcaacc aggaaatata cccacagcta tatactcctg aaatatttta tgaggcctgc
      361 agaaatctcc agtcatttta tgctcatcag gagacgtcac aaaacgctaa atatagtgca
      421 atagacaaga aaaaaagtca tcttaaccag gaaatcaaaa agctaattaa aaaaaatatt
      481 ttccctgaac tatacaatga gcaatgcaat aaaatcccct cctcatcgac agatgataac
      541 cagcaaataa catggcagaa ttttaaaact agcaattcgg catattcaca actatgtgaa
      601 aagttatctt tatttaaaag cgtgcccagt cggttaatag aaaaatcagc ttactgctca
      661 actgaaaaca tgatcaccaa taaatttgat gttgtctttt ctttctgcgg agataatata
      721 aaggaattta tacttttatt accctataat aaatcgctgg agatgtatga acttaacgag
      781 caaaagatcc agtatgtgac tacccctaat attaatataa ataaactgtt gctatcaaat
      841 ataacaataa aaaagagcaa cctttcacat gggtattatt ttggttgtgt attatccaat
      901 atatcatgtt ttgaaagcga cttatcaaac accatctttt caaatggaga aataaacaac
      961 ctcttcatta aaaaaagcaa catatttgga acatcgttca ctaataccat gataaaaaat
     1021 ttgcgctgtg aagatattat gcctggccga tggaccactc aacttgtcaa taagcactta
     1081 ggttacagat acacaggagt attcaaaacc ctggcaagta ttgatgacaa accgtcccgt
     1141 ttcgaaattt tgataccatt ggtacaaact ttagttcgcg acaatgtaaa gctaaataat
     1201 gatgtgtata aagaactgaa aaagtttatg cacgattacg acaaaacatc cccagaaatg
     1261 agaaaatacc ttcagagcat caacgagtct atgttcctta tgaaaaaaat atctcatcaa
     1321 gattaaaaca ccccaaaaaa gaagtaattt cagaatgaaa ttacctgtga taattattaa
     1381 ttaactgcag agaaataaaa aataatctga agcatgctct gaaacatcct actctgtcac
     1441 tctaatgatg gcaccttgct cttcttataa agaagggaac aattaagaa
//
LOCUS       DAZFFG010000297         1480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFFG010000297 DAZFFG010000000
VERSION     DAZFFG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.300"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            164..1375
                     /gene="bluF"
                     /locus_tag="ACHJ7J_005096"
     CDS             164..1375
                     /gene="bluF"
                     /locus_tag="ACHJ7J_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO5003168.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADRSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALVFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANGLVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
BASE COUNT          427 a          302 c          309 g          442 t
ORIGIN      
        1 tttaactgac aacctataca aaaattttcg ccaactcgtg ttcaaatttt tccatttttt
       61 atcaatgtaa agaaacagtt atgttttggt attaaaagaa agacaccgga agatatgtct
      121 gttaccgtct tactctcgcc tctcccatta ccctggacag ttaatgctta ccacccttat
      181 ttatcgtagc catatacgtg acgacgaacc tgtcaaaaaa atcgaagaaa tggtttcgat
      241 agcaaatcgc aggaacatgc agtctgacgt aacagggatc ttactgttta atggttctca
      301 ttttttccag cttctggaag gtccggaaga acaggttaaa atgatatatc gggctatatg
      361 ccaggatcca cggcactata atattgttga gctgctgtgc gattacgcgc ctgctcgccg
      421 ttttggcaaa gcgggaatgg aattatttga tttacgcctg cacgagcgag atgacgtttt
      481 acaggccgta ttcgacaaag gcacatcaaa atttcagcta acttatgatg acagagcgct
      541 acaatttttt cgtacttttg tccttgcaac cgaacaatca acctatttcg agattcctgc
      601 tgaagactcc tggcttttta tcgctgacag atctgataaa gaacttgatt cctgtaccct
      661 ttcaccaaat ataaacgacc actttgcctt tcatcctatt gttgatccct tatcgcggcg
      721 gataatcgct tttgaagcca ttgtgcaaaa aaatgaagac agcccgtcag ctatagcggt
      781 tgggcagcgt aaagacgggg aaatctacac agcggatctc aaaagtaagg cgcttgtatt
      841 cgcgatggca cacgcacttg agctcggtga taaaatgata tctatcaatc tattacctat
      901 gaccctggtt aacgagcctg acgcagtctc ttttttactt gatgaaataa aggccaatgg
      961 tctggtgcca gaacaaatca tcgttgaatt tactgaaagt gaagtcatat ctcggtttga
     1021 tgagtttgcc gaagcggtta aatcgctaaa ggctgccggt atcagtgtag caattgatca
     1081 ttttggcgca ggttttgctg gtttgttact cctgtcacgc ttccagcctg acagaattaa
     1141 aatcagtcag gaattgatta ccaatgttca taaaagcgga ccacggcagg caattattca
     1201 ggcgatcata aaatgctgta catcacttga aattcaagtc agtgctatgg gcgtggcaac
     1261 accagaagag tggatgtggc ttgaatccgc aggaattgag atgtttcagg gggatctgtt
     1321 tgctaaagct aaattgaatg gtatcccttc agttgcgtgg ccggagaaaa aataattttc
     1381 agcacattat tttgcatgtt ttctggaaat catcgtgaaa atgaatacgt tatgttttaa
     1441 tttaatctac attaaaaata atgtttcatc caatttagct
//
LOCUS       DAZFFG010000298         1456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFFG010000298 DAZFFG010000000
VERSION     DAZFFG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.301"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            26..>1456
                     /locus_tag="ACHJ7J_005097"
     CDS             26..>1456
                     /locus_tag="ACHJ7J_005097"
                     /inference="COORDINATES: protein motif:HMM:TIGR04393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO5003169.1"
                     /translation="MNKIYKVIWSMALNTWVVVSEISSFIKKKSCCLCSGLILVSPFS
                     LGADIECLYSSCYADDNIIITTERNLIDEAKQQYPSSRYAALFVGQKSETNLRVISGG
                     KVTASTLQVGVNNNSVVDIRDGGEIIIDDYGQNITSLFNIGQGGEGVVNIDGAGSVLN
                     FIPGDEEINVGYSSTGYLNITNGGKFISTPSDSSWGSIYAGGRGGASGVIKVSGKGSL
                     LEPAYRLYVGVWGDGKLEVTSGGIVNTQNFQMGYIASGEAIVSGTGSQLNIDNTAAIV
                     STTNINARGTLIINNGGKVTADAIALGNTDNYDKPSTGVAEVLIGGEDNNIIQEAGII
                     DANKFIFRGENTSLTIKHASDNFNLVSDISSQGLSSYKKYYYGSINAVHGLTTLSGNN
                     SGYTGALNISSPARISVTEQNNLGNATIANDGTLSITSQGNWTFSNTMTGLGFLDVNT
                     ANNTFSFQNATNTAGFRGTLMLSDTSF"
BASE COUNT          454 a          258 c          329 g          415 t
ORIGIN      
        1 tcttatctaa aaataggata tataagtgaa taaaatatat aaagttatct ggagtatggc
       61 tcttaataca tgggttgtgg tatctgaaat aagctctttt ataaagaaga agtcctgttg
      121 tctctgttca ggtctgattc ttgtatcccc cttctctttg ggggcggata ttgaatgcct
      181 ttattcctca tgttatgcag atgacaatat tattatcaca acggaacgaa atctgattga
      241 tgaagcaaaa caacagtatc cctcatcaag atatgctgct ctttttgtag gtcaaaaaag
      301 cgaaacaaat ctccgggtta tttcaggggg taaagtaacc gcttccactc tacaggttgg
      361 tgttaacaac aatagtgttg tcgatatcag ggatggtggg gagattataa ttgatgacta
      421 tggtcagaat ataacatctc tttttaatat tggacaaggt ggagaggggg ttgttaatat
      481 tgatggtgca ggcagtgtgc ttaattttat tccgggcgat gaagaaataa atgtaggtta
      541 ctcttcaact ggttatctga atattaccaa tggtggtaaa ttcatttcaa caccaagtga
      601 tagctcatgg ggctctatct atgcaggagg ccgtggcggg gcttcgggag taattaaagt
      661 atccggaaaa ggaagtttgc ttgaacctgc atatcgattg tacgttggtg tatggggaga
      721 cggaaaactt gaagtgacca gtggaggtat agttaatacc caaaattttc aaatgggata
      781 tatagcttca ggagaggcaa tcgtatcagg aacaggctct cagctcaata tagataacac
      841 tgcagcaatc gtttcaacca ctaacattaa tgcacgtggt actcttatta ttaataatgg
      901 cggtaaagta acggctgacg ctatagcttt aggaaacact gataattatg ataagcccag
      961 taccggggta gcagaagtcc ttattggagg agaggataat aacatcattc aggaagcagg
     1021 gataattgat gctaataaat ttatattcag aggagaaaac acatcattaa caattaagca
     1081 tgccagcgat aattttaatc tggtatctga tataagcagc cagggactct ccagctataa
     1141 aaaatattat tatggcagta ttaacgccgt acatggatta acaaccctta gcggcaataa
     1201 ctctggttac accggggctc tcaatatcag ttctccggcc cggattagcg tcacagaaca
     1261 gaataatctg ggcaatgcca ctatagctaa tgacggtact ctgagtataa ccagccaggg
     1321 taactggact ttcagtaaca cgatgacagg gctggggttc ctcgatgtta ataccgcaaa
     1381 caatactttc agttttcaga atgcaacaaa cactgccgga ttccgcggaa cactgatgct
     1441 gtccgatacg tcattt
//
LOCUS       DAZFFG010000299         1404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFFG010000299 DAZFFG010000000
VERSION     DAZFFG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.302"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..337)
                     /locus_tag="ACHJ7J_005098"
     CDS             complement(<1..337)
                     /locus_tag="ACHJ7J_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO5003170.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLN"
     gene            complement(792..1247)
                     /locus_tag="ACHJ7J_005099"
                     /pseudo
     CDS             complement(792..1247)
                     /locus_tag="ACHJ7J_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(1289..>1404)
                     /locus_tag="ACHJ7J_005100"
     CDS             complement(1289..>1404)
                     /locus_tag="ACHJ7J_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO5003171.1"
                     /translation="AAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
BASE COUNT          371 a          336 c          283 g          414 t
ORIGIN      
        1 gatttagatt tttcagatat ggtcttatga tctttccctt acttaataaa tagcatgtat
       61 aggattcgga aagtccagca tctattttgt acctcattat attagataag gatgtgagaa
      121 attccttgtt atcattcgca gccatatatg ccagtccgag ttcagcctct gcggccttac
      181 aggcaaaata gctaagtgaa cacgcttttc gtccctcttt agtactggct gatattagtg
      241 cccataatat tgctttgtct gccaaatcta actctctctg aaattgtctt gcagtaactg
      301 cactcgcaga aatagagaaa aaaatacaat tgttaataac tttgccatat tttttagtcc
      361 gtaactgtta atgtgctatc tggctctgaa aatattaact catttaattt ttgcatgtca
      421 ttagatgaac cagttataca accctcggaa cagaatcgag gtcctaaaga cggaccgaaa
      481 gcgtttaaac aactatgtgt taaaaatcca tcacgattat atgtttctgt atttgctgac
      541 ggtactaaac gtataccatt aggttttcta ttggttgctc caggaccatt aacattccag
      601 ctccataccc cttgaggtat tggacctcta ttttgtaagt gtgtacaacc aggattattt
      661 ttacattgca tattattacc attattacca gaggcgacag gaatagtaag cacatcataa
      721 gagttatcat cattttgcgt atctgaaacg catgaaagca tacttaaagt tatgctgtat
      781 aaacaggtcg ctaatcccaa cgggtcaata tcagttatcg gattcaacgg atactgataa
      841 aaattccatc cccctttcag cccaatcgga tcctgagtga tatatcgccc ctgcagcggg
      901 tcataatagc ggtggcggtt gtaatacagg ccggactcct catcatactg ctgccccggc
      961 aggcggataa gctgctgcag ctgatgcggg ttctcttcat tcagcaggtt gccccactca
     1021 tcgtattctg cgcaccattc tgtagcacct tccgtgctga taagcgccag cggcaggccg
     1081 cgatggtcgc agtggtacag gtggattttt cgcgccggcg tgtacaccgg gtccatctgg
     1141 ttttgcatct gcgccaccgt caggccgcac gatgccagcc agcggcggct ttcctcactc
     1201 acccggtcag ccaggatttc actttccagc cggtcgagca tctgcaccgt gccggcggga
     1261 atcccccact gtgcataggc aactcacgct atgaacgctt cagcttatca atagcggaag
     1321 ttataatttc attgtcatca aacttgtaca acatagtatc taaaacagga agtagcgttt
     1381 tatcacctaa ttcacccgca gctt
//
LOCUS       DAZFFG010000300         1379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFFG010000300 DAZFFG010000000
VERSION     DAZFFG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.303"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>1379)
                     /locus_tag="ACHJ7J_005101"
     CDS             complement(<1..>1379)
                     /locus_tag="ACHJ7J_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5003172.1"
                     /translation="VKTEQLQQFIKIMEQKAQLPIYIDKNNLKESIFSVLHNDPQHVD
                     KDQHLPCDKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQLTIQDNNNKTE
                     TFSFTNLQWENLLQYYRENHQQPNIAGSRNLTDNIDKIKNTISTSEIIECASPEIRSS
                     VLNDLYSIANFLPDNNLTPNESWKRFCDTCERFYVAQKSITGDKSERLTRKLSISDAG
                     ITMTFTIGDVVINTISTAIPEDATGQRCIEGLNLAEMDLTDIDLSKMALRNVNFNGSI
                     LRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDFRKVRHLTYVNFKNANLRNSNFS
                     GKVLTGVNFTGSDLSNAYLEHIDFTTVILYETPATPATPATPATPATPATPATPATSE
                     TPKIPEIPGTHKIPATPKTPATPQIPEIPETPKVILTGAILNYSDLSGKDLSEYNLTG
                     IICMYTNFS"
BASE COUNT          366 a          228 c          294 g          491 t
ORIGIN      
        1 tgaaaagttg gtatacatgc agataatacc agtaagatta tattctgaaa gatcttttcc
       61 cgatagatcg gaataattta gtattgcgcc agtaagaatt actttaggtg tttcaggtat
      121 ttcaggtatt tgaggtgttg caggtgtttt aggcgttgca ggtattttat gtgttccagg
      181 tatttcaggt attttaggag tttcagatgt tgcaggtgtt gcaggtgttg caggtgttgc
      241 aggtgttgca ggtgttgcag gtgttgcagg tgttgcaggt gtttcatata gaatcacggt
      301 tgtgaaatct atgtgttcaa gataagcgtt actaaggtca cttccagtaa agtttacgcc
      361 agtgagaact tttccgctga agttactgtt ccgtagattt gcgtttttga aatttacata
      421 agtcaagtgg cgaactttac gaaaatcatt attttctaat gaggcatttt cgaattctgc
      481 attacgcaga tcacaatcgg taaaatccac gccttcacag atcgtaccgg agaacttggc
      541 atttctaaga atgctgccat taaaattgac attccttagc gccattttcg acaagtctat
      601 gtcggttaaa tccatctctg ctaaattcaa cccttcgata caccgttgac ccgttgcatc
      661 ttcaggaata gcagtgctaa tagtattgat gacaacatca cctatcgtga aggtcattgt
      721 aattcctgca tcagagatag agagttttcg cgtaagacgt tcactcttat ctccagtgat
      781 actcttctga gcaacgtaaa agcgctcgca tgtgtcacaa aatcttttcc agctctcatt
      841 tggggtcaga ttattgtccg ggaggaaatt agcaatgcta taaagatcgt tcaggacgct
      901 acttcttatt tcaggagagg cgcattcaat aatttcagag gtggatattg tatttttaat
      961 tttatctata ttatccgtga gatttcgtga tcctgctata tttggctgct ggtggttttc
     1021 tctatagtat tgcagcaaat tttcccattg taagtttgta aacgagaaag tttcagtctt
     1081 gttattgtta tcctgtattg ttaattgatt ttctactggg tcaaaagaaa tcatgaagtt
     1141 attcaggtgt tgatattgat gagtggcatc taatttcact tcaaatgaat ttgaactttt
     1201 gcaggcatgt tttaaaaact tatcacaggg aaggtgttga tctttatcta cgtgttgtgg
     1261 gtcattgtgc aaaacagaga aaatactctc tttcaaattg ttcttgtcaa tatagattgg
     1321 tagttgcgct ttttgttcca ttattttaat aaactgctgc aattgctcgg ttttaacat
//
LOCUS       DAZFFG010000301         1362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFFG010000301 DAZFFG010000000
VERSION     DAZFFG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.304"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            370..888
                     /locus_tag="ACHJ7J_005102"
     CDS             370..888
                     /locus_tag="ACHJ7J_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5003173.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPSGQRAHKPFIFTVALNKAVPLLYNALASGEMLPKVELHWWRTS
                     VEGKQEHYFTTRLTDATIVDMNLHMPHCQDPAQREFTQLLAVSLAYRKVEWEHIKSGT
                     SGADDWRAPLEA"
     gene            1109..>1362
                     /locus_tag="ACHJ7J_005103"
     CDS             1109..>1362
                     /locus_tag="ACHJ7J_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5003174.1"
                     /translation="MSLNGLRFTLEIDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKKATLTIWQGAVAQRYVTGVVAGFGMQEN"
BASE COUNT          343 a          292 c          377 g          350 t
ORIGIN      
        1 aatacatcat aaggaattcc tttaaggaat gtcttaattg cgtgctggaa cgatggcgga
       61 gtaagtgtcg taataatttg tttttgacgc ggctgtggat ttttatgggg ggggaagata
      121 cgttatggat aattctgctg ttgaagttga tatattttta gcttatgtga ccgtgttctg
      181 aaattgacta aaaatatatt taaacaccat aaaagagttt ttcagtaggt gctaattacg
      241 tgtgcctgta actcgaataa agaaaagggt gtggttcctg atgtgagccg catcacactc
      301 taacccgtcg gggattaacg acgggttgtt cgtaaaacag cagttgataa tttcacaagg
      361 agttcataaa tgccaacccc atgttacatt tcaattacag gtcagaccca ggggaacatc
      421 actgccggtg ctttcaccgc agattctgtc ggcaatatct acgtgcaggg acacgaagat
      481 gaaatgctgg tgcaggagtt tctgcataac gttactgttc caaccgatcc acagtctggt
      541 caaccttccg ggcagcgcgc gcacaaaccg ttcattttca ccgtggcgct gaacaaagcc
      601 gttccgctgc tgtacaacgc gctggcctcc ggtgaaatgc tgccgaaagt ggagctgcac
      661 tggtggcgta cttcagttga aggcaagcag gagcactact tcaccacacg tcttactgac
      721 gcgacgattg tggacatgaa cctgcacatg ccgcactgcc aggaccctgc acagcgtgag
      781 tttacgcaat tgctcgcagt atcactggca taccgtaagg ttgagtggga gcacatcaaa
      841 tccggtactt ctggtgccga tgactggcgt gcaccgctgg aagcataaat ctgagtcaac
      901 aacatcctgc cttcgtgcag gatgttgttt ttgtacgtgt aaaaaatgag tgtcagtaag
      961 gagttacgct ttcatgaggg agatggcgga tgccggaaac ccgggcggta gcgtacctga
     1021 aaatgtaaag atgagtgtaa cgctgcactg gcaccctttg ctgcatggtg agaatcattt
     1081 ggtccgtgaa taaatcaggg aggtcattat gtccttaaac ggtctgcgct ttacgctgga
     1141 gattgacggc caggagccgg acacctttgc agtggtgaat ttcaggttaa tccagaacca
     1201 gtcatatccg tttgtgatga gtgtggatgt tgccagcgac tcttttatgc agacggcgga
     1261 gatgctgctg gagaagaagg cgacgctgac catctggcag ggcgcggtgg cgcagcgtta
     1321 tgtcaccggc gtggtggcgg gttttggtat gcaggagaac aa
//
LOCUS       DAZFFG010000302         1344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFFG010000302 DAZFFG010000000
VERSION     DAZFFG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.305"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1255
                     /locus_tag="ACHJ7J_005104"
     CDS             <1..1255
                     /locus_tag="ACHJ7J_005104"
                     /inference="COORDINATES: protein motif:HMM:NF015371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5003175.1"
                     /translation="VALEDASTTKKGIVQLSSATNSTSETLAATPNAIKAVMDEVGKK
                     AAMHSPAFSGTPTTPTPPDDADGAEIANVAFVRKLIAGLVGSSPAALDTLNELAVALG
                     NDPHFATTVTNALSGKQPLSDILTAISKLTVQADKLLYFNTGGNITLSPLSVKARSLL
                     AQLTSEDMRQVLEISDSVKLQRNIYDRTAGMGALPGAFGFGALTSEIKYFSAGEGQSE
                     LITWCMQTPPGRYLVEQRNNPDFNPIIPGITFTGVLEIKISDKVYSHEEHQGKVKLLI
                     FYGKYGDIYRTVLYADRTTPYLSPWSSGYINRRGLASLLGNDSLQPEVGNILLAAYYG
                     TSDSDMARTLQRGKVYPGSRLSHVEFSAGVATGAGIKESTPTCHVSGMSFSSYLPGSY
                     MALSGSGGATRDNGSSLIGLFVRVW"
BASE COUNT          368 a          287 c          357 g          332 t
ORIGIN      
        1 tgtggcgctt gaggatgcaa gcacaacgaa aaaggggata gtacagctca gcagtgcgac
       61 caacagcacg tcagaaacgc ttgctgcaac cccaaatgcc atcaaagccg ttatggatga
      121 ggtagggaaa aaagcggcga tgcacagccc ggcgttctca ggaacaccaa ccacaccaac
      181 accgccggat gatgcggatg gagctgaaat agccaatgtg gcgtttgtgc gtaaactgat
      241 tgccgggctg gttgggtcat caccggcggc gcttgatacg ctgaacgagc tggcggtggc
      301 gctgggtaac gaccctcatt ttgcaacaac agtcactaat gcactgtcag gtaaacagcc
      361 gcttagtgat attttaacag caatcagcaa attaacagtg caggcagata aacttctgta
      421 ctttaataca ggagggaata tcacgctgtc tccgttgtca gtaaaagccc gttcactgtt
      481 ggcacaactc acatccgaag atatgcgcca ggtgctggaa atctcagata gcgtgaaatt
      541 acaaagaaat atttatgacc gaactgcagg gatgggcgca ttacctggcg catttggttt
      601 tggagcactg acttcggaga ttaaatattt tagtgcaggt gaaggtcagt ctgagcttat
      661 cacctggtgt atgcagacgc cgccagggcg ctaccttgtg gagcagagaa acaatccaga
      721 ttttaatccc atcattccgg gtattacttt taccggggta ctcgaaatta aaatttctga
      781 caaggtatac agtcatgaag agcatcaggg taaagtaaaa ttactgatat tttacggtaa
      841 atatggcgat atatacagaa cagttcttta tgcagatcgc actactccgt acctgtcccc
      901 ctggtcctcc ggatacataa acagaagagg gctcgccagt ctgcttggta acgatagcct
      961 gcaaccggaa gtcggtaata ttcttcttgc agcttattac ggtacatcag acagcgatat
     1021 ggccagaact ttgcagagag gaaaggttta tcctggctca aggttgtcgc atgtggagtt
     1081 ttcagcaggg gtggcgacag gggctggtat taaagagagt acgccgacgt gccatgtcag
     1141 tgggatgtcg ttttcatctt atcttcctgg ttcatacatg gcgttatccg gttcgggtgg
     1201 tgccacccgg gacaacggaa gcagtcttat aggtttgttt gtgcgggtat ggtagatgat
     1261 gaaaatacag gatattaaaa atctccgcac gcttgaaagc ggtgcagtgg attgtgagat
     1321 attgttttct ggcatgaatg aata
//
LOCUS       DAZFFG010000303         1334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFFG010000303 DAZFFG010000000
VERSION     DAZFFG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.306"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            3..737
                     /locus_tag="ACHJ7J_005105"
     CDS             3..737
                     /locus_tag="ACHJ7J_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5003176.1"
                     /translation="MTGCGNLSNVSPEGTTDEPVWPSPDRTMFSHSGTQKGSWPVPEK
                     LRLIKAGMNKDQIYQLAGRPHFGEGIAGVREWDYLFNFTGPDGDYVCQFKILFDRNMD
                     ARSFFWKPENCSQEQEVNRYERTLQGDILFDHDSSVLTEKGREQLMIVAEELRKEGVR
                     RFIIEGHTDHTGSEPYNNALSEKRAGTVKGWFISQGFDAGNIEIRGKGDKEPLVVCEN
                     TGDEVMKECLRPNRRVVITAEEEGDR"
     gene            827..1006
                     /gene="tnpB"
                     /locus_tag="ACHJ7J_005106"
                     /pseudo
     CDS             827..1006
                     /gene="tnpB"
                     /locus_tag="ACHJ7J_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1011..1334
                     /locus_tag="ACHJ7J_005107"
                     /pseudo
     CDS             1011..1334
                     /locus_tag="ACHJ7J_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          376 a          268 c          370 g          320 t
ORIGIN      
        1 ttctgaccgg gtgcggtaac ctcagtaatg tttcaccgga aggaacgact gacgaacctg
       61 tatggccttc tcctgacagg acaatgttca gtcacagcgg aacccagaaa ggctcctggc
      121 ctgtaccgga gaagctgcgt ctgataaaag ccggaatgaa taaggatcag atatatcaac
      181 tggcaggtcg tcctcatttt ggtgaaggga tagcgggtgt gagggaatgg gattatctct
      241 ttaatttcac cggacctgac ggagattatg tctgccagtt caagatcctt ttcgacagaa
      301 acatggatgc aaggtcattt ttctggaaac cggaaaactg cagtcaggaa caggaagtaa
      361 acagatatga gagaacatta cagggggata ttctgttcga tcatgattca tcagtactga
      421 cagaaaaagg acgtgaacaa ctgatgattg ttgcagaaga actcagaaaa gaaggagtca
      481 ggcgtttcat tatagaaggt catacagatc atacggggtc agaaccatat aataacgcac
      541 tgtcagagaa acgggccggc acagtcaaag ggtggtttat aagccagggt tttgatgcag
      601 gaaatataga gatccggggt aaaggtgaca aggaacctct ggttgtctgt gaaaatacgg
      661 gggatgaggt tatgaaggaa tgtcttcgtc caaaccgaag agtggtgatt actgctgagg
      721 aagaaggaga caggtaaata gatctgatat accagagctt gttatttata tgcttttatt
      781 tttcggggcg cgttagcgcc ctttttattc ttacccttaa ccatctatgg gggatggtgg
      841 tgattgccaa tgcaagaata tgcgccggga catcgacaca ctgatacagt acgtccagaa
      901 cgaacttaac gctgcatggc atgatggtgc tgcttttgtc ttcaccaata aagcccgctc
      961 ccgcataaaa gttctccgct gggataaata tgatgtatgg ctgtgtaacc gtgaagaaaa
     1021 tcgccgggtt gtaccgtctt gataagaaga tcagtagccg cctcgtggaa aaatccgcca
     1081 gtggcaacag cgttatgccc gtccgctact ggaagatctg tggtcatggc ttgaagagca
     1141 ggaaccgcaa tgttctccgg gaagggcatt acacaaagcc attgtctatg cgctgtctca
     1201 tcgcgtggaa ctaagccgct tcctggaaga tggtgcggtg ccgctggata ataatgtgtg
     1261 tgaacgggcc atcaaaaacg tggtcctggg cagaaaatcg tggctgttcg ccggttcgcg
     1321 gatggcagga gaac
//
LOCUS       DAZFFG010000304         1330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFFG010000304 DAZFFG010000000
VERSION     DAZFFG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.307"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            69..473
                     /gene="stpA"
                     /locus_tag="ACHJ7J_005108"
     CDS             69..473
                     /gene="stpA"
                     /locus_tag="ACHJ7J_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO5003177.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(520..1044)
                     /gene="ygaP"
                     /locus_tag="ACHJ7J_005109"
     CDS             complement(520..1044)
                     /gene="ygaP"
                     /locus_tag="ACHJ7J_005109"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO5003178.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1054..>1330)
                     /locus_tag="ACHJ7J_005110"
     CDS             complement(1054..>1330)
                     /locus_tag="ACHJ7J_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5003179.1"
                     /translation="ASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGLSA
                     SATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          349 a          327 c          322 g          332 t
ORIGIN      
        1 aataagtgat cgcttacact acgcgacgaa atactttttt gttttggcgt taaaaggttt
       61 tctttattat gtccgtaatg ttacaaagtt taaataacat tcgcaccctc cgtgcgatgg
      121 ctcgcgaatt ctccattgac gttcttgaag aaatgctcga aaaattcagg gttgtcacta
      181 aagaaagacg tgaagaagaa gaacagcagc agcgtgaact ggctgagcgt caggaaaaaa
      241 ttagcacctg gctggagctg atgaaggctg acggaattaa cccggaagag ttattgggta
      301 atagctctgc tgctgcacca cgtgctggta aaaaacgcca gccgcgtccg gcgaaatata
      361 aattcaccga tgttaacggt gaaactaaaa cctggaccgg tcagggccgt acaccgaagc
      421 caatcgctca ggcgctggca gaaggtaaat ctctcgacga tttcctgatc taattactga
      481 ggccggatgt cgctgccagg gcgcgtccgg cgtcgcttct caagctcgtt ggttccacgg
      541 catcttatct aacaaccttg ccattccaca aaaaccgctg attcctgcaa acagtaaccc
      601 ggcaccaaca aagccactta ataagaagaa accgctattt acggtataac ccagtacaac
      661 gccgattaat attaaaccac ccgcagcgat ctgcacctgg cgcattaacg gcaagggttg
      721 agatttattt accgctactg gcaatccagc tcttttccag ccatcaatcc catcttcgag
      781 taaaaatatt tctgcggggg cggcaatcgc tgctaattta tcggcgttat tactggtgcg
      841 tttacctgcc tggcagtgga atataatttg ctcgcgacgt aatttagccg gaagacctga
      901 ctgttccagc acggataatg gagccagatc tgcttcagga atatgttcac gaagatattc
      961 atcagcgtca cgaatatcga ttaactttgc gccgcgtgcg attaattctt gtgcatcatg
     1021 cggcgaaatg gttgtcaaag ccatagtgac tccttacgga caatagacat ttttcagggt
     1081 ggcgataatg gcatttaccg cctcattttt aatggaatat agaatgcgtt gggcatcccg
     1141 ttggctgtcg ataagccctt cgtcccgcat acgagcgaga tgctgtgatg tcgcagaggc
     1201 gctcagtccg gtaatgcgcg tcagctctcc cgcgctggtg ccgggggaac cgctaagcat
     1261 gcacagaatc agcaaccgtt tagggtggct cattgctttc aataaggccg ctgcctgttc
     1321 ggcactggcc
//
LOCUS       DAZFFG010000305         1311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFFG010000305 DAZFFG010000000
VERSION     DAZFFG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.308"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..280
                     /locus_tag="ACHJ7J_005111"
     CDS             <1..280
                     /locus_tag="ACHJ7J_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO5003180.1"
                     /translation="LESQAIETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNY
                     VDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSKWTFQE"
     gene            294..533
                     /locus_tag="ACHJ7J_005112"
     CDS             294..533
                     /locus_tag="ACHJ7J_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO5003181.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            553..885
                     /gene="sctI"
                     /locus_tag="ACHJ7J_005113"
     CDS             553..885
                     /gene="sctI"
                     /locus_tag="ACHJ7J_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO5003182.1"
                     /translation="MSVSNMPPIDRVEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARNIVA
                     AVETLTRS"
     gene            882..>1311
                     /gene="sctJ"
                     /locus_tag="ACHJ7J_005114"
     CDS             882..>1311
                     /gene="sctJ"
                     /locus_tag="ACHJ7J_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO5003183.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALP"
BASE COUNT          437 a          216 c          275 g          383 t
ORIGIN      
        1 attggaaagt caggctattg aaacgttgtc ggcgataggt ctttcttatg aaaaatataa
       61 aacagctaac aatgtgtatt tcaatatcat tggtacgtta agtgacagtg aattaaataa
      121 aattaataac tatgttgacg aatattataa acaatggggt aaacaatatg taagatttaa
      181 tgtgaatttg aaaaatcagg acacaaataa tagttcattt agctacggag ataaccgatt
      241 cgagaagtct caaggtagca agtggacatt tcaggaataa ttgaggagga cttatggcag
      301 attggaatgg ttatattatg gatatcagca aacaatttga tcagggcgtt gatgatctga
      361 accagcaagt tgaaaaagcg ttggaggatt tagcaaccaa tccttccgac ccgaagttcc
      421 ttgccgaata tcaaagtgca ttagctgagt atacattata tcgaaatgcg caatctaacg
      481 ttgtaaaagc gtataaagat ctcgattctg caatcataca aaacttccgc taaactggat
      541 aaggattttg ttatgtcagt aagtaatatg ccacctattg atagagttga gcaatcaact
      601 gctcatgaaa tacagcaagc gaaagtgatt gatttaaatg accgggttct caatcttgac
      661 aacccggatg ataaaatgat ctccgctttt gccaactatg ctgtacaaac tgaaaattgg
      721 cagcaaaatg cgttacaagc attaaggtct gataaaaagg ggttaactcc tgaaaaatta
      781 ttagtcttgc aggatcatgt gcttaattat aatgtcgagg tttcattggt gggaacatta
      841 gcaagaaata ttgtcgctgc agttgagacg ttgacccgct catgaaatat atatcattat
      901 tattgtttat cctgctgttg tgcggctgca agcagcagga attattgaac catcttgatc
      961 aacagcaagc taatgatgtc cttgctgttt tacaaagaca taatattaat gctgaaaaga
     1021 aggatcaagg caaaacagga ttttcaatat ttgtcgaacc aacggatttt gcttctgcgg
     1081 tggattggct aaaaatctac aatctcccgg gtaagcctga tattcaaata tcgcagatgt
     1141 tccctgcaga tgcactcgtt tcatctccac gagcggaaaa agcaaggcta tattctgcta
     1201 ttgagcagcg gttagagcaa tctctcaaaa ttatggatgg cattgtttct tcgagagtac
     1261 atgttagtta cgatgttgat actggcgata gtggtaaaac tgctttacca a
//
LOCUS       DAZFFG010000306         1292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFFG010000306 DAZFFG010000000
VERSION     DAZFFG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.309"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(89..316)
                     /gene="mchB"
                     /locus_tag="ACHJ7J_005115"
     CDS             complement(89..316)
                     /gene="mchB"
                     /locus_tag="ACHJ7J_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO5003184.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(333..542)
                     /gene="mchI"
                     /locus_tag="ACHJ7J_005116"
     CDS             complement(333..542)
                     /gene="mchI"
                     /locus_tag="ACHJ7J_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO5003185.1"
                     /translation="MSYKKLYQLTAIFSLPLTILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRKILVWSGWKNGS"
     gene            651..887
                     /locus_tag="ACHJ7J_005117"
     CDS             651..887
                     /locus_tag="ACHJ7J_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003186.1"
                     /translation="MIKDNSKVDDSADRLSFTVTLFVANSILPDLTQTKTKENDCYLL
                     FKKKCHIVHIKSRGILRYVTDAIVSQDLMVVAHV"
     gene            955..1200
                     /locus_tag="ACHJ7J_005118"
     CDS             955..1200
                     /locus_tag="ACHJ7J_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003187.1"
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKYDNFPIEVESSVELET
                     HCITNNKSASGIVTHDYDADYICGCGEIMCPGCGHDL"
BASE COUNT          432 a          267 c          239 g          354 t
ORIGIN      
        1 taaatggatc ttctatctgc tttaaacgaa agggatatat tttacaggca ctaccgaaaa
       61 agtgcctgta aaataaataa aattataatt agctaccgcc accagcagaa gaactggcac
      121 taccacttcc cacggtcgag ccaattgctg ttgtcaaacc ggcagatacg gctccaacta
      181 caaccccaag tggaccacca ggtattccgg cgagagctcc aacaatagct gcagcacctg
      241 cggcattagc tgaagtcgct ggcgcacctc ccgccccgga aatatatctt aactgtgatt
      301 ctgttatttc tcgcatataa actccatcat atttaacttc cgtttttcca gcctgaccag
      361 accagaatct ttcgaatccc atgaatcagc tcaataaaac caagaaatat tataaagctt
      421 agaaaaacgt caacataaga attcccttcg ccaacaatcc gaagggatga aagtgaaacc
      481 aataagatag taagaggtaa actgaatata gccgtcaatt ggtacagttt tttataactc
      541 attaatacct acctgttgct ttattttcat tatgaaaagt taccaaatct tagcgcttca
      601 gtcaggggag tcaggtcatc gataaagaat attccagtag agtggcgctc atgatcaaag
      661 acaacagcaa agttgatgac agtgctgacc gactgtcatt tacagtaacc ctgtttgtag
      721 caaattccat cttacctgac ctcacccaga caaagacaaa agagaatgat tgttatttac
      781 ttttcaaaaa aaagtgtcat attgtccata taaagtcaag aggtatttta cgttatgtaa
      841 ctgatgcaat tgttagtcag gatctcatgg ttgtcgctca tgtataacct gagacttgaa
      901 cgtacactaa tagcactgac ggtcatacat cgggaaatgt gaggaggcat aagaatgaat
      961 tgtgataata atcacagaaa tgaagaattc attgttacct ttgataaagg caacaagcaa
     1021 gacaattcaa gacgaaaata cgataatttt cctatagagg tagaatcctc cgtagaactg
     1081 gagacacact gtatcacaaa taataagtcg gcttccggta tagtaacaca tgactatgat
     1141 gccgattata tttgtggttg tggtgaaatt atgtgtcctg gttgcggtca tgacctataa
     1201 caaacatttt tgcaaactga tacctgatca ggtacaagcc tgttgattat tatgctgaat
     1261 attccagtta tcaatccatc gatatgaaca tt
//
LOCUS       DAZFFG010000307         1288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFFG010000307 DAZFFG010000000
VERSION     DAZFFG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.310"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(460..1095)
                     /locus_tag="ACHJ7J_005119"
     CDS             complement(460..1095)
                     /locus_tag="ACHJ7J_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO5003188.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          350 a          274 c          257 g          407 t
ORIGIN      
        1 cgtaacgcct ctataaataa tccgcaaaat atcgtcgtag cggcactcga tatttttaca
       61 ttcctgtatc aagtactgaa aatcatttca tcgctacttc caattccttt ctgcatggta
      121 agtagtgagg acgaaaagaa gaatatgata aatattggtt cgtggtaatc agagagagtc
      181 gcgacatttg tgatgttatt cttacaactg taagaagcgc cggtgattta caagtaaggg
      241 tatgattacg ctttttaaag cgtaccataa aatataacat attaaataat ttgtgatgac
      301 acgggattat tgcggtttac tttgaggaat ataaaatgcg agtttataga acagcgagcg
      361 ccaaataatt ttatgaccac aacctgtcgt attgtggtca taaaaaattg tcggctggag
      421 ggagttaaat cctttacctc aatatactgc ctgtcagcat tagttcacaa acagtgcttc
      481 gctatcgacg atgattccat cttccgcacc tttaagcgcc aggtttgcct cgtagtattg
      541 ctttttatca agcagtttct gcgcatcagc aagtgcatta cgagtctgtt taagcggcat
      601 cagatattgg ttttccatta caccaacacc ggccaggcga agctcttcca ttgcgccctt
      661 tttatcccct ttcgccattt tttcattggc aatttttatc gcggcttctt tttcgggtgt
      721 cgctacataa ctctcagaaa ttccgacaga tgcattaatg acaatatatt ggtcatcatt
      781 gacattggtc tttttacctg gcttagcgaa ctttgcccat tcggtactgt catctgacaa
      841 caaagctgaa gcttcattgg tcagttcttt tgccttttcc ggatcgccat gaaacagagc
      901 caggcgcgct acctgcacgt cacgcatcgc atataaacct tgttcagaga tacgctctgc
      961 tgcagctgcc gttttctggg tttgctgaac ttgctgagtt tgctgttgtg ccgctaccgg
     1021 agcattatca gcagcccata cagttgaaga tgccagaatt gctgtgacca tcgtagccat
     1081 aattaaacgt ttcatactta cctcattctt taacagtttt agaaaacgcc gccatcatat
     1141 tgggtgagca tgaaatgaga aatcagagag caacggtcat tttgaaaggg ttatcttaca
     1201 gttgtagtta ttatcgcaag aagtatttta gtcttacaat tgtaggaata tgcttcctgt
     1261 cacagagaac tattcttatg taccacaa
//
LOCUS       DAZFFG010000308         1280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFFG010000308 DAZFFG010000000
VERSION     DAZFFG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.311"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(107..1207)
                     /gene="ompC"
                     /locus_tag="ACHJ7J_005120"
     CDS             complement(107..1207)
                     /gene="ompC"
                     /locus_tag="ACHJ7J_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5003189.1"
                     /translation="MKLKIVAVVVTSLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          367 a          285 c          263 g          365 t
ORIGIN      
        1 ggtgaatgac cagaaatatc aaaaattgct catttttact taaggagtca tacagcagca
       61 cttaaatcaa aaaccagccc agagaagggc tggtcaggag aggatgttag aactgatagg
      121 taatgcccgt agcaacgatg ttatcggtgc gaatatctac ggcacgggta aagtcgcttt
      181 catcaatcag gttgatttta taatcaacat aggtcgacat atttttgttg aagaaatacg
      241 tcgcaccaac gtcgatatac tcaatcatgt cctggtcgcc gtagcggccc agatctctgc
      301 ctttcgattt caggtaagcg agtgaggggc gcaagccgaa gtcgaattga tattgcgcca
      361 ccacttcaaa actttgtgct ttgttagaaa tataaccatc gccataggtg gtcatgttta
      421 atgtttcagc gtaattagct gcaaagtaga tgttgttggc atcatatttt atacctgtag
      481 cccacagttc tgcattctta ccggaggcat tgagaggatt agcagcggct ctttcctgcg
      541 catttgtccg atcggatttg gtatacaccg ctccgatacc aaagccatca taaacataag
      601 ttgaggagat accgaaaccg tcaccgttgg cacgcgtgta gtcagcacca taaagatgac
      661 tgttgtcagt acgttcattt ttgccttgat attgcgcggc aaagttcagc ccttcaatta
      721 agccaaagaa atcgtagttg cgataggttg ctacaccggt ggtacggtat gtcatgaaga
      781 catctttctg actccaggta tcgccaccaa attctggcaa catatcagtc catgaaccaa
      841 tgtcatagcc gacaccgtag ttacggccat agtccagaga gccaaattca ctaattttta
      901 aaccagcata ggccagacgg gtcttgttgc cgcgcgagcc gttggcttca tcgttatagc
      961 ctttaaaatc atattcccaa tgaccaaaac caatcatttg atcattaatt tgcgtttctc
     1021 ctttaaagcc gagacgggca taagttttat caccatcgtc acgcttatca tcagtaaaat
     1081 aacgtagagc ggtaaccttg ccataaaggt ctagtttatt accatccttg ttatagactt
     1141 cggcagcgtg tgctacgttc gcagctaaca aactagttac aaccaccgca actattttta
     1201 atttcattta acagaatcct tttaattatc gttaaacgta ttttctaaac gaattttaaa
     1261 cggcgtcatt tataaatgac
//
LOCUS       DAZFFG010000309         1278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFFG010000309 DAZFFG010000000
VERSION     DAZFFG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.312"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..1271
                     /locus_tag="ACHJ7J_005121"
     CDS             <1..1271
                     /locus_tag="ACHJ7J_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003190.1"
                     /translation="AGALNAPIQESIEAYEAIVTPEEIKYIRTGLEELKTAHYSLTVF
                     LNLERLFPVVNIISSYYRKYLNDGIRPNNISSMLCRYLYEPQSNDIFCHSNIINSIVE
                     YYYVRAIKPLSLNISNDVMLCVDKLKDIVFNFNNKTIISEADLINIAVVLKKEPDNHC
                     LMPYLELHKVIHLISHGETDEAYEMLKRVSLDDLPSGYLTAAFLILRTALRIKRERKK
                     IKNGVFSSDITAILESQGIYTDYVPVIKDYTNNGIDGSLTKNSFLSGTILPDANNLTI
                     MRIVRMYNNMVRRISHWNDLYHDGVFPESVYGLLDKVDAILGKILNIIFFENITSRRN
                     LAFILKNKKILTTSELNDNLIGILINCPLITCVQNLEILIQYLRCPGEELRNIIISID
                     RKSWNLICGALKILEEDRRGRAEITQKDGK"
BASE COUNT          441 a          228 c          237 g          372 t
ORIGIN      
        1 aagcaggtgc gctgaatgct cccatacagg agagcattga ggcttacgaa gccattgtta
       61 caccagaaga gattaaatac atcagaactg gtctggaaga attaaaaacc gcacattatt
      121 cactgaccgt attccttaac cttgaacgtc tattccctgt tgttaatata atctcgtcat
      181 attacagaaa gtatcttaac gatggaataa ggccaaataa tatctcgtct atgctatgca
      241 ggtatcttta tgaaccccaa agtaatgaca tcttctgtca cagtaacatc attaattcaa
      301 ttgtggagta ctactacgtg cgggcaataa aacccctatc actgaatata agcaatgatg
      361 ttatgctgtg cgtggataaa ttaaaagaca ttgtatttaa ttttaataac aaaaccatta
      421 tttctgaagc ggatttaata aatattgcag ttgtattaaa aaaagaacct gacaatcatt
      481 gtctgatgcc atatcttgaa ctgcataagg tgatacattt aatcagccat ggagaaactg
      541 acgaagctta tgagatgctt aaacgggttt ctctggatga tttaccttca ggatatctga
      601 cagctgcttt tttaattctc cgtactgcat tacgaataaa gagagaaagg aaaaaaatca
      661 aaaatggtgt tttctcctcg gatataacag caatactgga aagtcagggg atctatactg
      721 actatgtccc tgtcattaag gactatacaa acaacggcat tgatgggtct cttacgaaaa
      781 attcattttt atctggcacc attttaccag atgccaacaa tctgaccatc atgcgtatag
      841 taagaatgta taataatatg gtcagaagaa taagtcactg gaatgacctt tatcatgacg
      901 gtgtttttcc ggagtctgtt tatgggctcc ttgataaagt tgatgccatt cttggtaaga
      961 tactcaatat tatctttttt gaaaatatca catccagacg gaatttagcc ttcatcctga
     1021 aaaataaaaa aatccttacc acaagtgagc tgaatgataa cctcattggt atactgatta
     1081 actgtccact tataacgtgc gtacaaaacc tcgaaatttt aatacaatac cttcgctgtc
     1141 cgggagagga gttaagaaat ataattattt caattgacag gaaatcatgg aatttgatat
     1201 gcggagccct gaaaatcctg gaggaagaca gaagaggacg agcagaaata acacagaaag
     1261 atgggaaata attgcata
//
LOCUS       DAZFFG010000310         1260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFFG010000310 DAZFFG010000000
VERSION     DAZFFG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.313"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>1260
                     /locus_tag="ACHJ7J_005122"
     CDS             <1..>1260
                     /locus_tag="ACHJ7J_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Contact-dependent inhibitor A"
                     /protein_id="HFO5003191.1"
                     /translation="EVNAALHAKDLTVTAGANRVTADGRVRALKGEGDVPKVAVDTGA
                     LGGMYARRIHLTSTESGVGVNLGNLYAREGDIILSSSGKLVLKNSLAGGNTTVTGADV
                     SLSGDNKAGGNLSVTGTTGLTLNQSRLVTDKNLVLSSSGQIVQNGGELTAGQNAMLSA
                     QHLNQTSGAVNAAENVTLTTTGDTTLKGRSVAGKILTVSSGSLNNGGTLVAGRDATVK
                     TGTFSNTGAVQGNGLKVTATDLTSTGSIKSGSTLDISARNATLSGDAGAKDSARVTVS
                     GTLENRGRLVSDDVLTLSATQINNSGTLSGAKELVASADALTTAEKSVTNSDGNLMLN
                     SASSTLAGETSAGGTVSVKGNSLKTTTTAQTQGNSVSVDVQNAQLDGTQAARDILTLN
                     ASEKLTHSGKSSAPSLSLSAPELTSSGV"
BASE COUNT          321 a          356 c          364 g          219 t
ORIGIN      
        1 gaagttaatg ccgcgcttca tgcgaaggat ttaactgtca ctgcaggcgc taaccgtgta
       61 actgcagatg gtcgtgtcag agccctgaag ggcgaaggtg atgtgccgaa agttgccgtt
      121 gataccggcg ctctcggtgg aatgtacgcc aggcgtattc atctgacctc cactgaaagt
      181 ggtgtcgggg ttaatcttgg taacctttat gcccgtgagg gcgatatcat actgagcagt
      241 tccggaaaac tggtcctgaa gaacagcctt gccggcggca ataccaccgt aaccggagcg
      301 gatgtctcac tttcagggga taacaaagcc ggaggaaatc tcagcgttac cgggacaacg
      361 ggactgacac tgaatcagtc ccgtctggtg acggataaaa atctggtgct gtcttcatcc
      421 gggcagattg tacagaacgg tggtgaactg actgccggac agaacgccat gctcagtgca
      481 cagcacctga accagacttc cggggccgtg aatgcagctg aaaatgtcac ccttaccacc
      541 acgggtgata ccacactgaa aggccgcagc gttgccggga aaatactcac cgtcagttcc
      601 ggcagcctga acaacggcgg gacactggtt gccgggcgcg atgccacggt gaaaaccggg
      661 acattcagta ataccggtgc cgtccagggg aacggcctga aagtcaccgc cactgacctg
      721 accagcaccg gcagtattaa aagtggcagc acactcgata tcagtgcccg caatgccaca
      781 ctgtccggtg atgccggagc aaaagacagc gcccgcgtta ccgtcagcgg tacacttgaa
      841 aaccgcggca gactcgtcag cgatgacgtg ctgacgctca gtgccacgca gataaacaac
      901 agcggtaccc tctccggggc aaaggaactt gtggcttctg cagacgcact gaccaccgcg
      961 gaaaaatcgg tcacaaacag tgacggtaac ctcatgctga acagcgcgtc ttccacactg
     1021 gcgggtgaaa ccagtgcggg tggcacggtg tctgtaaaag gcaacagtct gaagaccacg
     1081 accactgcgc agacgcaggg caacagtgtc agcgtggatg tgcagaacgc acagcttgac
     1141 ggaacacagg ctgccagaga catcctcacc ctgaacgcca gtgaaaagct cacccacagc
     1201 gggaaaagca gcgccccgtc gctcagcctc agtgcgccgg aactgaccag cagcggtgtg
//
LOCUS       DAZFFG010000311         1247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFFG010000311 DAZFFG010000000
VERSION     DAZFFG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.314"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1016)
                     /locus_tag="ACHJ7J_005123"
     CDS             complement(<1..1016)
                     /locus_tag="ACHJ7J_005123"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO5003192.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREG"
     gene            complement(1027..>1247)
                     /locus_tag="ACHJ7J_005124"
     CDS             complement(1027..>1247)
                     /locus_tag="ACHJ7J_005124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003193.1"
                     /translation="IIQEAQKAHKNNNIKHLTTTQEFSIALFFNEKILFQQYLTINNI
                     RILLFKHESFALAYFIDKSYFFNAIQSF"
BASE COUNT          310 a          304 c          310 g          323 t
ORIGIN      
        1 tagccttcac gaccgaggcg caggaattca tagtactgtg caattacctg acccgccggg
       61 cgggagaagt tgatggcgaa agtaccaatc tgacccccca ggtagtcaac gttgaacacc
      121 agttcctgcg gcagcgcttc ttcgtcacgc cagataaccc agccgcagcc cagcggagcc
      181 agaccgaatt tatggcctga agcactgatc gatttcacac gtggcaggcg gaagtcccag
      241 acgatatccg gggcgacgaa cggtgccagg aagccaccac tggcggcgtc gatgtgcatg
      301 tcgatgtcga taccggtgtc ggcctggaat ttatccagcg catcgtgcag cggttgcggg
      361 aactcatagt taccggtgta ggtcacgccg aaagtcggca ccacgccgat ggtattttcg
      421 tcgcaggctt caatcatgcg tttcggatcc ataaacaact gaccggggcg catagggatc
      481 tcacgcagct ccacatccca gtagcgggcg aatttatgcc agcagatttg caccgggccg
      541 cacaccaggt ttggtttatc cgttggtttg cctgcagctt ccatacgctt gcgccaacgc
      601 catttcatcg ccatcccgcc gagcatacag gcctcggaag aaccaatggt gttggtgcca
      661 acggcctgac catttttcgg cgcaggcgca tgccacagat cggcaaccat atttacgcaa
      721 cgcaggtcga tggctgcgga ttgcggatat tcttctttgt cgatccagtt tttgttaatg
      781 gataaatcca tcaacttgtg gacattttcg tcgtcccagg tctggcagaa agtggccagg
      841 ttctgacgag cgttgccatc aagatataat tcgtcattga taatctgaaa tgcgacatcg
      901 tcgcgcattt cgtgcagcgg aaaacgtttt gattctgcga tagtggaaat agacttcgca
      961 ccaaaacgtg aatcgagtag ttccgacctt aaatccgtta cttgcttctt atccatttta
     1021 aactccttaa aatgattgga tcgcattaaa aaagtaggat ttatcgataa agtaagcaag
     1081 tgcaaaggac tcgtgtttaa ataacaaaat cctaatgtta tttatcgtga gatattgttg
     1141 aaataatatt ttttcattaa aaaacaatgc aatagagaat tcttgggtgg tggtaaggtg
     1201 ttttatgttg ttatttttat gcgctttctg tgcctcctga attatca
//
LOCUS       DAZFFG010000312         1246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFFG010000312 DAZFFG010000000
VERSION     DAZFFG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.315"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..125)
                     /gene="hokD"
                     /locus_tag="ACHJ7J_005125"
     CDS             complement(<1..125)
                     /gene="hokD"
                     /locus_tag="ACHJ7J_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5003194.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVA"
     gene            complement(491..>1246)
                     /locus_tag="ACHJ7J_005126"
     CDS             complement(491..>1246)
                     /locus_tag="ACHJ7J_005126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003195.1"
                     /translation="ERTKWVMFLPCSKEEVEPRHVFDIVFGVLCLEKAGINPQDISIY
                     IDSPNRNLDGFFSSASKYPYTSKASAEFFNDLHDNNYSNIVMFVTGHGCPQGLDAKNP
                     IPPHQLLKALKGTPNLNNAIVYLGQCYAGTFNFVGAGKRKDGEPEVILIGATNLTESL
                     SIGTTETFLDGNEFPWPANIFLLHVFKWMSEPSDIDGDGRYTVMDSYKHAGIFTNLVN
                     KKTKTDMFGEIINIHAECNKLMALASSGTGGIG"
BASE COUNT          348 a          279 c          233 g          386 t
ORIGIN      
        1 gcgacctccg tctggccggt tcggattcgt acctcgcaga ggtctttcct cgttaccagt
       61 gccgtcacta tgacggttaa acagatgacg atcagggcga ttaacatcgc cttttgctgc
      121 ttcatagcct gcttctcctt gacctttcgg tccgtaagag gcaatctata tgtgacgagc
      181 atataggggc ctcacttcga tttatagtcg ggtggggctt ttatctatct gccgttggtg
      241 ttcatgcccg aggcagatag cctcaagcac ccgcagcaat tctacttaac tctccttttc
      301 ccgcaaaccg ttttttctca cgggaataag tcgttattgt aattaactga catgacgatt
      361 aattaaaatt caattttttg cgctggtctt gcgtttaata tccagcattc ttgatgggtg
      421 tggtgcgcaa tcaataagtt ttggagttga gttttttttg ccttataatt taacttattt
      481 gtcgtatcaa ctatccaatt ccccccgtcc ccgaagaagc cagagccatg agtttattac
      541 attctgcatg gatattaatt atctcaccaa acatatctgt ttttgttttt ttattcacaa
      601 ggttagtaaa aatacccgca tgcttatagg aatccattac ggtataacgg ccgtcaccat
      661 ctatatcaga cggttccgac atccatttaa aaacatgaag gagaaaaata ttggctggcc
      721 aaggaaattc attcccgtca agaaacgttt cagtagtccc tatactcagg ctttcagtaa
      781 gattagtcgc cccaattaaa ataacttcag gttcaccatc ctttctttta cccgctccaa
      841 caaaattgaa tgtacctgca taacactgcc caagataaac aatagcattg tttaaattag
      901 gcgttccttt taaagcttta agaagttgat gaggcggaat aggatttttt gcatcgagcc
      961 cttgtgggca accatgaccg gtaacaaaca taactatatt actgtagtta ttatcatgca
     1021 agtcattaaa aaattctgct gatgctttcg acgtataagg atacttcgag gctgatgaaa
     1081 aaaaaccatc taaatttctg ttagggctgt caatatatat tgatatatcc tgggggttta
     1141 taccagcctt ctcaaggcat agcacaccaa aaacaatatc aaaaacgtgg cgcggctcaa
     1201 cctcttcttt agaacaaggc aaaaacataa cccattttgt acgctc
//
LOCUS       DAZFFG010000313         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFFG010000313 DAZFFG010000000
VERSION     DAZFFG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.316"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..225
                     /locus_tag="ACHJ7J_005127"
     CDS             <1..225
                     /locus_tag="ACHJ7J_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFO5003196.1"
                     /translation="VNYSLYRKAVEDLATRGFSIVNSQGSESRNPALSAKADAERIMI
                     KMASLLGFDPVSRRRNPPETEEEDELDRLA"
     gene            209..>1233
                     /locus_tag="ACHJ7J_005128"
     CDS             209..>1233
                     /locus_tag="ACHJ7J_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5003197.1"
                     /translation="MTAWHEYAEGVKNGKITACKRLKQAVKRYFYDLENPLYTFDPEV
                     VERFIAFSRVCPHVKGAMRGSPIKLEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTVAAILANWFLVMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRKRVNI
                     QAHKVIHPKTNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEMNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMLTKRFNIWCQGATPWMGNGAWAECAGT
                     FAEADLYG"
BASE COUNT          319 a          295 c          354 g          265 t
ORIGIN      
        1 gttaactact ccctataccg caaagccgtc gaagaccttg cgacgcgcgg cttcagcatt
       61 gttaacagtc agggcagcga gagcagaaat cccgccctga gcgcaaaggc tgacgcagaa
      121 agaataatga tcaaaatggc ttctttgctg ggttttgacc cggtaagccg tcgcagaaat
      181 ccaccggaaa cagaggaaga ggacgagctt gaccgcctgg catgagtacg cagaaggcgt
      241 aaaaaacggc aaaattacgg cctgtaaacg actgaaacag gccgttaaac ggtattttta
      301 tgaccttgaa aacccccttt acacgttcga tccggaggtc gtggagcggt ttattgcctt
      361 ttccagggtg tgtccgcacg taaaaggcgc aatgcgcggt agcccaatta aactggagcc
      421 gtggcagcag ttcgcctttg cgtgcatcct gggctttaag gttaaggcca ccgggcggcg
      481 caaatacacc agtgcattca ttgaagtacc gcgaaaaaat gccaaatcca cggtcgccgc
      541 tatcctggct aactggtttc tggttatgga aaacgggcag caggatattt acaccgccgc
      601 cgtgagtcgt gatcaggcgc ggatcgtgtt tgatgatgcg cgtcagatgt gccttttatc
      661 ccgaccgtta cgaaagcggg taaatattca ggcgcacaag gtgatacacc cgaaaaccaa
      721 cagcctgtta aagccactgg cagcaaaagc tgcaaccatt gaggggacaa acccgagtct
      781 tgccattgtg gatgaatatc acctgcaccc agacaacggg gtttattccg cacttgagct
      841 ggggatgggg gcgcgtccgg aggggctgtt atttgccatc accacatcgg ggagcaacgt
      901 tgtttcagcc tgtaaacaac actatgacta ttgctgtcag atactggatg gtgaagagat
      961 gaacgaatcc atgttcgttc tgatttacga gctggatgat gaaagcgagg ttgacgatcc
     1021 ggcgatgtgg ataaaggcga atccaaatat cgatgtttcc gtcgatcgtg aaaaactggc
     1081 ctcaaccatc cagaaagcgc ggggtattcc gtcgcagtgg gtggaaatgc tcaccaagcg
     1141 attcaatatc tggtgtcagg gggctacgcc gtggatgggt aacggtgcat gggcggagtg
     1201 cgccggaacg ttcgcggagg cggatttata cgg
//
LOCUS       DAZFFG010000314         1232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFFG010000314 DAZFFG010000000
VERSION     DAZFFG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.317"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            1..1060
                     /locus_tag="ACHJ7J_005129"
                     /pseudo
     CDS             1..1060
                     /locus_tag="ACHJ7J_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          395 a          210 c          289 g          338 t
ORIGIN      
        1 gtcagatatt ctactgttga ctatttgtgc cgtcatttct ggtgcagagg gttgggaaga
       61 tatagaggat tttggtgaaa catatctcga ttttttgaag caatatggtg attttgaaaa
      121 tggtattcct gttcacgata ccattgccag agttgtatcc tgtatcagtc ctacaaaatt
      181 tcacgagtgc tttattaact gaatgcgtga ccgccattcc tcaaatgata aagacgttat
      241 tgcaattgat ggaacaacgc tccggcactc ttatgacaag agtcgctgca ggggagcgat
      301 tcatgtcatt agtgcgttct caactatgca cagtctggtc ctcgggcaga tcaagacgga
      361 tgagaaatcc aatgagatta cagctattcc tgaacttctt aacatgatgg atattaaagg
      421 aaaaatcatc acaactgatg cgatgggttg ccagaaagat attgcagaaa agatacaaaa
      481 acagggaggt gattatttat tcgctgtaaa gggaaatcag gggcgactaa ataaagcctt
      541 tgaggaaaaa ttcccgctga aagaattaaa taatccaaag catgacagtt acgaaataag
      601 tgaaaagagt cacggcagag aagaaacccg tcttcatatt gtttgcgatg tccctgatga
      661 acttattgat ttcacgtttg aatggaaagg actaaaaaaa ttatgcatgg cagtctcctt
      721 tcggtcaata atagcagaac aaaagaaaga gcccgaaatg acggtcagat attatatcag
      781 ctctgctgat ttaactgcag agaagttcgc cacagcgatc cggaatcact ggcacgtgga
      841 aaataagctg cactggcgcc tggacgtgat aatgaatgaa gacgactgca gaataagaag
      901 aggaaatgca gcagaattat tttcagggat aaggcatatc actattaata ttttgacgaa
      961 tgataaggta ttcaaggcag ggctaagacg taagatgaga aaagcagcca tggacagaaa
     1021 ctacctcgcg tcagtccttg cggggtgcgg gctttcgtaa tcttgccctg ttttcggggc
     1081 ggctcctata taactaatgt gaggttttat gagtatccga catcaatata atgaagcttt
     1141 aaataagctg gaagtagatg ttaatgatgg tttgcgagat ttaataaaca tatattgtgc
     1201 tgctattgat tcttttgaga atgatatcgt tg
//
LOCUS       DAZFFG010000315         1221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFFG010000315 DAZFFG010000000
VERSION     DAZFFG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.318"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..232
                     /locus_tag="ACHJ7J_005130"
     CDS             <1..232
                     /locus_tag="ACHJ7J_005130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003198.1"
                     /translation="EGVIQVPESPDAPEALPGCRSISDDDLCAWCTRLLYRPGELSLC
                     RLSTGNGIWPSVCDRNDYAYGCPEFQPNIIHP"
     gene            326..805
                     /locus_tag="ACHJ7J_005131"
     CDS             326..805
                     /locus_tag="ACHJ7J_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5003199.1"
                     /translation="MKIASQNTTALSSTTAPENDQSRLVATLIPDEQRISFWPQYFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            821..>1221
                     /locus_tag="ACHJ7J_005132"
     CDS             821..>1221
                     /locus_tag="ACHJ7J_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5003200.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALCHNAAA
                     VILAHNHPSGEVTPSKADRLITERLVQALALV"
BASE COUNT          264 a          356 c          323 g          278 t
ORIGIN      
        1 tgaaggcgtg attcaggtac cagaatcacc cgacgccccg gaggcgctgc cggggtgtcg
       61 ttccatttct gatgatgacc tgtgcgcctg gtgtacccgt ctgctttacc gtcccggaga
      121 actcagtctg tgcagactga gtacaggcaa tggcatctgg ccatccgtct gtgaccggaa
      181 cgattacgcg tacggctgtc cggaatttca gccgaatatc attcatccct gatattaccg
      241 ggtctcacgg tcagtgatga acgtgatgtt gtcagccccc ggcctttatt tcctctcatt
      301 tatacacatt gtaaggattt cactcatgaa aatcgcttct cagaacacaa ctgctctttc
      361 ttcaaccaca gcaccagaga atgaccagtc ccgacttgtt gccaccctca tccctgatga
      421 acagcgcatc agcttctggc cgcagtattt tggcctcatt ccacaatggg tgaccctgga
      481 gccccgtgtc ttcggctgga tggaccgtct gtgtgaagac tactgcggtg gtatctggaa
      541 tctgtacacc ctgaacaacg gtggggcatt tatggcaccc gaaccggatg acgatgatga
      601 cgaaacatgg gtactgttca atgccatgaa cggtaaccgc gctgaaatga gcccggaagc
      661 cgccggcatt gccgcctgtc tgatgacgta cagccatcac gcctgtcgta cggagtgtta
      721 tgccatgacg gtccattatt accggctgcg ggattacgcc ctgcagcatc cggaatgcag
      781 cgccattatg cgcatcatcg actgaatgga ggcagggaca atgcagcaac tttcctttct
      841 gcctggagag atgacgcccg gcgagcgcag cctcattctg cgggccctga aaaccctgga
      901 ccgccatctt catgaacccg gagtggcctt cacctccacc cgtgcggcac gggaatggct
      961 gattctgaac atggcgggac tggagcggga agagttccgg gtgctgtatc tgaacaacca
     1021 gaatcagctg attgccggtg aaaccctctt caccggcacc atcaaccgta cggaagttca
     1081 tcctcgggag gtcattaaac gtgccctgtg ccacaatgcc gctgccgtga tactggcgca
     1141 caatcacccg tccggtgaag tcacacccag taaggctgac cggcttatta ctgaacgtct
     1201 ggttcaggca ctggccctgg t
//
LOCUS       DAZFFG010000316         1210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFFG010000316 DAZFFG010000000
VERSION     DAZFFG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.319"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>1210
                     /locus_tag="ACHJ7J_005133"
     CDS             <1..>1210
                     /locus_tag="ACHJ7J_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003201.1"
                     /translation="KCAGIWRKNTKKSHALILNLIMFCEMFLSSLSSLVVVNDPKKMK
                     RIFPLFIVSENINIHAEPDIEYFRVIDRAFDEVANYTGRIFSYLRTHEPLKLTSCVNK
                     QTLLSMGGYLNEWNVFDSLSRVRDFFRLSSAVFTKLDNNIYSLEVDSFCLYRDYEIAR
                     NRLMMRASHLYSEVHEFSNKHFHLNSWVKDHMPSYLNSDGVFSSFHLSELENISPDDL
                     HEEYGNISLFNWVHAYQCLVELSKEEMSKRFSSTKPIPLQLDRWLIIKSRESWLSFFQ
                     RKGIAADAAKKLIDYFTFNSKSHDLNDCPFIPCMDGLCLMPALIANSSVTRSLMSLFG
                     SKKISQASKGRFHEQQFIKQVRDAGIKASPIDAHANYQCDCVILLDDCLIFTELKSNG
                     QPIYYGKYYQQ"
BASE COUNT          392 a          190 c          222 g          406 t
ORIGIN      
        1 aaatgtgctg gtatttggcg taaaaataca aaaaaatcac atgcattaat acttaatctc
       61 atcatgtttt gtgagatgtt tttatcatca ttatcatcct tggttgtggt caatgatcct
      121 aaaaaaatga agaggatttt tccattattt atcgtttctg aaaatattaa tattcatgct
      181 gaacctgata tcgaatattt tcgagttatc gatagggcat ttgatgaggt agccaattac
      241 accggacgaa tattttctta tttacggact catgagccat tgaaactaac ttcttgtgtg
      301 aataaacaaa ccttgctttc aatgggaggg taccttaatg agtggaatgt ttttgactca
      361 ttaagcagag taagagactt tttcagacta agtagtgcag tatttacaaa attggataat
      421 aacatttact ctcttgaggt tgatagtttt tgtttatacc gagattatga gattgcaaga
      481 aatagactaa tgatgagagc gtcacatcta tatagcgaag ttcatgaatt tagtaacaaa
      541 cattttcatc taaatagttg ggtaaaggat catatgccat cttacttaaa ttctgatggc
      601 gtttttagct catttcatct aagcgagttg gaaaatatat ctcctgatga ccttcatgaa
      661 gaatatggga atatatcatt atttaattgg gtgcacgctt atcaatgttt agtcgagtta
      721 tctaaagaag aaatgagcaa aagattttca tcgacaaaac ctatacccct acagttggat
      781 cgctggctga tcataaaaag ccgtgagagt tggttatcat tctttcaacg taaggggatt
      841 gcagcagatg cagcaaaaaa actgatagat tattttacct tcaattcaaa atcacatgat
      901 cttaatgatt gcccatttat tccctgtatg gacggtttat gcttaatgcc tgcattaata
      961 gcaaatagct cagtgactcg ctctctaatg tcattatttg gttccaaaaa aatttctcaa
     1021 gcaagtaaag ggcgatttca tgagcaacaa tttataaaac aagttcgtga tgcggggatt
     1081 aaagcttctc caattgatgc tcatgctaat tatcagtgcg attgcgtgat tctcctggat
     1141 gattgtttga tttttacaga actaaaatca aatggtcaac caatatatta tggtaagtac
     1201 tatcagcaag
//
LOCUS       DAZFFG010000317         1181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFFG010000317 DAZFFG010000000
VERSION     DAZFFG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.320"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            52..576
                     /gene="lpfA1"
                     /locus_tag="ACHJ7J_005134"
     CDS             52..576
                     /gene="lpfA1"
                     /locus_tag="ACHJ7J_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFO5003202.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFTGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLKTGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            641..1181
                     /locus_tag="ACHJ7J_005135"
                     /pseudo
     CDS             641..1181
                     /locus_tag="ACHJ7J_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
BASE COUNT          325 a          249 c          273 g          334 t
ORIGIN      
        1 ttaatacatc aagattttct ttttaatgta attttttaaa tggagttttt catgaaaaag
       61 gtcgtttttg ccttaaccgc actggcgtta acttctggca ctgtattcgc agcagagtct
      121 ggtgacggca cggttaaatt tacaggcgag atcgtggatt caccttgcgt actgtccgtt
      181 gactctcaga accaggaagt tgtgttgggt caggtacaaa aatctgtatt tgctgcggtt
      241 ggtgacaaat ccccggcgaa acctttcgaa atcaaactgg aagattgcga taccacaacg
      301 atgaaaaaag ccaatgtgag ctttactggt gttggtgatg cagataaatc tgatctgatt
      361 tctgttagca ctgaagcagg tgcggccaaa ggtgttggta tcggtattta tgataacagc
      421 aacaccctgg tcgctcttaa cggtggtaaa gcaagtgttg acctgaaaac aggccagacg
      481 gttctctact ttaccgccaa ctacgtttca actcttgcaa cggtaacgac cggttacggc
      541 aatgcgcagg tcgatttcaa cctgtcttac gagtaatcgt ccgttttaca aatggcggcg
      601 taattgccgc cattagtcgt caaagttcat ggatcgaatg atgaaaagta aatttgttgc
      661 gttggctctc tctctgttcc tttcacagtc agttctggcg ggtggcgtcg gacttagctc
      721 tactcgcgta atttatgatg gcagtaagaa agaagcctcg ctgaccgtac agaataaaag
      781 taagacggaa gagtttctta tccaatcctg ggtggatgat gccgcgggca gcaaaaaaca
      841 ccgtttatta ttacgccccc actattcaag cttgatccag aaaagaacaa tattttgcgt
      901 attgtgaata ttacgcccgg cctgccgcag gatcgcgaat cggtctactg ggttaatgtg
      961 aaagccattc catctaaaag tgacgacagc gaaaacaaaa atgttttgca gattgccgtt
     1021 cgcacccgaa tcaaactttt ttatcgcccg gcaggtttaa agggtgatgt aaaaaccgcg
     1081 ccaaatgaac tgcgatttac gcgtaacggt aatcagctcc gcgttgataa tccgacagtt
     1141 tttaatatta cctttaatca attttttgcg aatgacaaag a
//
LOCUS       DAZFFG010000318         1176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFFG010000318 DAZFFG010000000
VERSION     DAZFFG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.321"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>1176
                     /locus_tag="ACHJ7J_005136"
     CDS             <1..>1176
                     /locus_tag="ACHJ7J_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Contact-dependent inhibitor A"
                     /protein_id="HFO5003203.1"
                     /translation="EVNAALHAKDLTVTAGANRVTADGRVRALKGEGDVPKVAVDTGA
                     LGGMYARRIHLTSTESGVGVNLGNLYARDGDITLDASGRLTVNNSLATGAVTAKGQGV
                     TLTGDHKAGGNLSVSSRSDIVLSNGTLNSDKDFSLTAGGRITQQNEKLTAGRDVTLAA
                     KNITQDTASQINAARDIVTVASDTLTTQGQITAGQNLTASATTLTQDGILLAKGHAGL
                     DAGTLNNSGAVQGASLTLGSTTLSNSGSLLSGGPLTVNTRDFTQSGRTGAKGKVDIMA
                     SGKLTSTGLLVSDDALVLKAQDVTQNGVLSGGKGLTVSAQALSSGKKSVIHSDATMTL
                     NVTTVALDGETSAGDTLLVQADKLSTAAGAQLQSGKNLSINARDARLAGTQAAQQTMV
                     "
BASE COUNT          292 a          320 c          359 g          205 t
ORIGIN      
        1 gaagtgaatg ccgcgcttca tgcgaaggat ttaactgtca ctgcaggcgc taaccgtgta
       61 actgcagatg gtcgtgtcag agccctgaag ggcgaaggtg atgtgccgaa agttgccgtt
      121 gataccggcg cgctcggtgg aatgtacgcc aggcgtattc atctgacctc cactgaaagt
      181 ggtgtcgggg ttaatcttgg taacctttat gcccgcgatg gcgatatcac cctggatgcc
      241 agcggcagac tgactgtcaa caacagtctc gccacggggg ccgtcactgc aaaaggtcag
      301 ggcgtcacct taaccggcga ccataaagcg ggaggtaacc tgagcgtcag cagccggagc
      361 gatatcgttc tcagcaatgg aacgcttaac agcgacaagg acttcagcct gaccgccggc
      421 ggcagaatca ctcaacagaa tgaaaaactg actgccggcc gggatgtaac gcttgccgcg
      481 aaaaacatca cacaggatac cgctagccag attaacgcgg cccgcgatat cgtgactgtc
      541 gccagtgaca cgctgacaac acagggacag ataaccgccg ggcagaatct cacggccagc
      601 gccaccacgc tgacacagga cggaatattg ctggcgaaag gtcatgcggg actcgatgcc
      661 ggcacgctga ataacagtgg tgctgttcag ggggcttccc tgacgctcgg cagtacaacg
      721 ctcagcaaca gtggctccct gctcagtggc ggtcccctga ccgtgaatac ccgcgacttt
      781 acccagagcg gccgcactgg cgcgaagggc aaagtggata tcatggccag tgggaaactg
      841 accagtacag gtttgctggt gagcgatgat gcactggtgc tgaaggcgca ggatgtgaca
      901 cagaacggtg tgctgtccgg cggcaaaggg ctgacggtca gtgcgcaggc gctgagttcc
      961 ggtaaaaaat cggtcatcca cagcgatgct acgatgacgc tgaatgtgac aacagtggcg
     1021 ctggacgggg aaaccagtgc cggtgacacc ctcctggttc aggcagacaa actgagtact
     1081 gcagcgggcg cacagcttca gagcggtaaa aatctcagca tcaacgccag agatgcacgt
     1141 cttgcaggta cgcaggcagc acaacagacc atggtg
//
LOCUS       DAZFFG010000319         1174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFFG010000319 DAZFFG010000000
VERSION     DAZFFG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.322"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1157)
                     /locus_tag="ACHJ7J_005137"
     CDS             complement(<1..1157)
                     /locus_tag="ACHJ7J_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003204.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIKNIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELKEFELADGNWLLDNAWYERNMAGFNEKLREN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANG"
BASE COUNT          313 a          295 c          291 g          275 t
ORIGIN      
        1 ccattcgccg ttgcatcgga aataaaaccg gtgtaagaac gttcataaat gtcctgacac
       61 agtgtttctg tttcgcgaca aagagaggag cagtaccatt ccggatccgg cttatgattt
      121 tccggggcat gatgtacttc tgtcagcccg caggaggcac agatacgaag ctcatccacg
      181 tggtggaatg atacaaaacg gcggccttct gaaaaggggt accagtcacc gccgtccagg
      241 caacgatcga gaaaatcgtc attcgcttcc ggtgataaat tcaggcggtt ccggaagagg
      301 gttttcagtt catcaggggt aaatgacagg ttctctctca gcttttcgtt gaaacccgcc
      361 atgtttcttt cataccaggc attgtccagt agccagtttc cgtctgccag ttcaaattcc
      421 ttcagttctc cccgggaaat ccgttgtgaa atgagtgtca gccagatgcg ctccgtcagc
      481 gtttcaggaa ggctgtaatc gttttgcaaa ctgctcagcc acgatttact gttgagcacc
      541 ggttgttctt ccagtgtctg gcggatggca ctggtcagtc ttttcagcgc ctggatctgg
      601 ctggccgccc atagcagcgt cactaatgca gccagccagt acaacaaccc accccaccac
      661 atcagtgagg gtgaaatatc gaactgcaat gcgatcaatg gtgcgtatgc agaacacgcc
      721 ataaggaaaa aagcagtata aaaacagcag taatacccgt taatttcctt gctgatatct
      781 tcaggtgtga gcccgttctg ctgaatgttt ttgatctgca cgggaagagc cagcagacca
      841 gcggcgagag cggtggtcag aataaatttt acagaatcaa aagtatgagc ctccagtgcg
      901 atatgtttac catcaagtcc ccacatgaag ctgatgatca ttccggcaat cgtcagaaat
      961 acgcctgcca gccagcgaat atggaataac cgggttatgc gttcagtgat atagccagcc
     1021 agtaaggccg gaccgaacag acacagcaat ataagccctg ccagcgcaga cgctgtgctg
     1081 gttaatgctc tgaacaaaag gcgaagaatg gcaaatcctg cgatcagcag aatcagcgcg
     1141 ccgactatct gtaacatagt cactccgtga tgaa
//
LOCUS       DAZFFG010000320         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFFG010000320 DAZFFG010000000
VERSION     DAZFFG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.323"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(235..>1159)
                     /locus_tag="ACHJ7J_005138"
     CDS             complement(235..>1159)
                     /locus_tag="ACHJ7J_005138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003205.1"
                     /translation="KTMEFKLDLKSNALDSFNEAIKKFQQGENGDYKAFKFAILHLSH
                     CFELTLKMYIQTLDENLIFSKCYRAVIERAKSDKVNLIEAFYKMEAEEFDFEELIKGH
                     KNPHTITLDQAISIATHEKCSITSVDLVDKELLDDIAWMKDLRNSIEHYEFSFTIKHV
                     RLAMGRLVRGLAEFSDIFGLYDLDKEVSKEISNSYNILSDEYEHNVHEGYLDVADARK
                     AAFLGVRPKFFELVNWKEYICDYCGHETMIQNNDSTSGYRCTYCGAEDSGSIEVTCDV
                     CGAEWPEEEMSSWLGEGTNTCPDCRDISNKP"
BASE COUNT          369 a          243 c          173 g          374 t
ORIGIN      
        1 ggatggacgc ctcccatccc gtttattcaa aggatgtaag gtttacagta tgtactccat
       61 gtaagaggat gaacagaaat ctattcctcc atattagcaa taatgtcata aattaggctt
      121 gccaattcgg ttgacaatat cttccgcctc tcacgctata atgccagaag cattatacat
      181 tataaaacat gagaggcgga caagaatgca tcttcaatca aggattaatc ttagtcatgg
      241 tttgttgcta atatctctac agtcaggaca agtgttagtc ccctccccaa gccaagaact
      301 catttcctct tcaggccatt cagcaccaca aacatcacat gtgacttcaa ttgagcctga
      361 gtcttcggca ccgcaatacg tacaacgata tcctgaagta gaatcgttat tttgaatcat
      421 tgtctcatga ccacagtaat cgcagatata ttctttccaa ttaactaact caaaaaactt
      481 tggtctaacc ccaagaaatg ctgcttttct agcatcagct acatcaagat aaccttcatg
      541 aacattatgc tcatattcgt cagaaagtat attataggaa ttagatattt ccttagaaac
      601 ttctttatct agatcgtata aaccaaatat atcagagaac tcagccaacc ctctgaccaa
      661 acgccccatt gccagacgaa catgtttaat agtaaacgag aattcgtaat gctcaattga
      721 atttctaaga tctttcatcc aagcgatatc atctagtaac tctttatcaa ctaagtccac
      781 agaggttatg ctacactttt catgtgtagc tatcgatatt gcttgatcca aagtaatagt
      841 atgaggattt ttatgtcctt taataagttc ttcaaagtcg aattcttcag cctccatttt
      901 ataaaaagct tcaataagat tgactttgtc acttttggca cgctctatta ctgctctgta
      961 gcattttgaa aaaatcaagt tttcgtctaa tgtttgaata tacattttca acgtcaactc
     1021 aaaacaatga gacaaatgta gtatagcaaa cttgaaagct ttataatctc cattttctcc
     1081 ttgctggaat tttttgatgg cttcattaaa actatctaaa gcattggatt ttagatcaag
     1141 tttaaattcc attgttttc
//
LOCUS       DAZFFG010000321         1139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFFG010000321 DAZFFG010000000
VERSION     DAZFFG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.324"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            96..>1139
                     /gene="tssD"
                     /locus_tag="ACHJ7J_005139"
     CDS             96..>1139
                     /gene="tssD"
                     /locus_tag="ACHJ7J_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO5003206.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTTESIGNRWQSGHENEIFTFSL
                     ANSITNTGLGSQLQGITFCKLIDKSTPLLINSINNNEQLFMEFDFYRINRFGRWEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITVSYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAVGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSKVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNTEDLLRMRKGYVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPY"
BASE COUNT          428 a          189 c          200 g          322 t
ORIGIN      
        1 tatacgccta caatttcctg gagtaaataa ataacaatta acaagcataa tagtgccatt
       61 gataaaatag catgccataa aaggactttt cagggatgag taatattgtt tacctgacag
      121 taacgggaga acaacaagga agcatctccg caggttgtgg gactactgaa tctataggta
      181 atcgctggca gagcgggcat gagaatgaaa tatttacatt ctcactcgca aatagcatta
      241 ctaatacggg gcttggttca cagttacagg gtataacatt ttgtaaatta attgataaaa
      301 gcactccatt acttattaat tccattaaca ataatgagca attatttatg gaatttgact
      361 tttatagaat caatagattt ggcagatggg aaaagtatta ttatatacaa ctaagaggcg
      421 cttttttatc ggctattcat catcagatca ttgaaaatca actggataca gaaacaataa
      481 ctgttagtta tgaatttatc ctctgtcaac atcttatcgc aaataccgag ttcagctatt
      541 tggcactccc tgaaaattat aaccgtttgt ttttaccaaa ttcaaaaaac caaacaaata
      601 atcgtttcaa aacgttaaac agcaaagctg ttggcaggct acttgctgct ggtggcgtat
      661 acaatgggaa cattgaaggt ttcagagata ctgcggaaaa actgggtgga gatgcaatca
      721 aaggctatga tcaaatacta aatgaaaaaa cagcgggcat agcgatagca acagcatcta
      781 ttcttttaac aaagcgttct aaagttgata catatacaga aataaatagt tacttaggca
      841 aacttagagg tcaacaaaaa cttcttgatg gcatagacat aatagaaata atatacatta
      901 agagaccttc aaaagactta gctaacttac gaaaggagtt taacaaaact gtaagaaaaa
      961 attttcttat caaacttgca aaaacctccg aagcatctgg aagattcaac accgaagacc
     1021 ttttaagaat gagaaagggc tatgttcctc taaattataa tgttcaccat aaactatctc
     1081 tagatgatgg tggtactaat gatttcgaaa atttagtatt aatcgaaaac gaaccatat
//
LOCUS       DAZFFG010000322         1138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFFG010000322 DAZFFG010000000
VERSION     DAZFFG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.325"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>1138)
                     /locus_tag="ACHJ7J_005140"
     CDS             complement(<1..>1138)
                     /locus_tag="ACHJ7J_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO5003207.1"
                     /translation="SSLSQNVTFVADVRTAQITDLAVIKDGSVADGATANTLQVKVTD
                     AFGNTLAGQTVSVLADNGATVAPTVITEPDGTVAISVTSQTAGISTVTATINNSTLSQ
                     NVTFIADVRTAKIADLVVIKDGSVADGAMANMLRARVTDAFGNALAGQTVSVFAGNGA
                     TTAPTVTTQPDGTVEISVTSQTAGTSAVTASINSSSQSRDVTFIADVRTAQIASLEVT
                     QDNAVADGAMADMLRARVTDAFGNALAGQTVSVMADNGAAVASTMTTKPDGTVEISVT
                     SQTAGISVVTASINSSSQSRDVTFIADIRTAQIADLVVIKDGSVADGAMANMLRARVT
                     DAFGNALAGQTVSVFAGNGATTAPTVTTQPDGTVEISVTSQTAGT"
BASE COUNT          183 a          394 c          312 g          249 t
ORIGIN      
        1 ggtgcctgcc gtctggctgg tgacagagat ctccaccgta ccgtccggct gcgtggtgac
       61 cgtcggagca gtcgtggcac cgttgcctgc gaacacgctg accgtctgcc cggcaagtgc
      121 attaccgaac gcatcggtga ccctcgcccg cagcatattc gccattgcgc cgtcagccac
      181 tgagccgtcc ttaatgacca ccaggtccgc gatctgcgcc gtcctgatat cggcgataaa
      241 cgtcacatcc cggctttgac tgctgctgtt gatgctggcg gtgaccacac tgattcctgc
      301 cgtctggctg gtgacactga tctccaccgt gccgtccggc tttgtggtca tcgtcgaagc
      361 gaccgctgca ccgttgtccg ccatcacgct gaccgtctgc ccggcaagcg cattaccgaa
      421 cgcatcggtc accctcgccc gcagcatatc cgccattgcg ccgtcagcca cggcgttatc
      481 ctgcgtcacc tccagactcg cgatctgcgc cgttctgaca tcggcgataa acgtcacatc
      541 ccggctctgg ctgctgctgt taatgctggc agtgaccgca ctggtgcctg ccgtctggct
      601 ggtgacagag atctccaccg tgccgtccgg ctgcgtggtg accgtcggag cagtcgtggc
      661 accgttgcct gcgaacacgc tgaccgtctg cccggcaagt gcattaccga acgcatcggt
      721 gaccctcgcc cgcagcatat tcgccattgc gccgtcagcc actgagccgt ccttaatgac
      781 caccaggtcc gcgatcttcg ccgtcctgac atcggcgata aatgtcacgt tctgactcag
      841 ggtgctgttg ttgatggttg cagtgactgt actgatcccc gccgtctggc tggtgacact
      901 gatcgccacc gtgccgtccg gctctgtgat caccgtcggg gcaactgttg caccgttgtc
      961 cgccaacacg ctgaccgtct gtccggcaag cgtattaccg aacgcatccg tgaccttcac
     1021 ctgcagcgta ttcgccgtcg caccgtctgc cactgagccg tccttaataa ccgccaggtc
     1081 cgtgatctgc gccgttctga catcagcgac aaacgtcacg ttctggctca ggctgctg
//
LOCUS       DAZFFG010000323         1125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFFG010000323 DAZFFG010000000
VERSION     DAZFFG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.326"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            34..662
                     /locus_tag="ACHJ7J_005141"
                     /pseudo
     CDS             34..662
                     /locus_tag="ACHJ7J_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004890961.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
     gene            complement(801..1079)
                     /gene="mcmA"
                     /locus_tag="ACHJ7J_005142"
     CDS             complement(801..1079)
                     /gene="mcmA"
                     /locus_tag="ACHJ7J_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin McmA"
                     /protein_id="HFO5003208.1"
                     /translation="MRKLSENEIKQISGGDGNDGQAELISIGSVAGTFISPGFGSIAG
                     AYIGDKVHSWATTATVSPSMSPSGIGLSSQFGSGRCTSSASSSAGSGN"
BASE COUNT          329 a          217 c          182 g          397 t
ORIGIN      
        1 ataacaaaaa aaatgcaact ataaaaaaca gtcatcagcg gctgcttttt aatgtttact
       61 ttatcatttt ctattatctt tattcctgca ttccttacaa acaggcagga acttatatcc
      121 aggggacttc ttgttccagc attatttctt gctgaatttc taattcttgt tccactgtat
      181 tttattttct tccgaaaaag agaaggatta ggaagaggtt cttttcgacc ggatgttttt
      241 tactcttttc tctttaataa tgattattca gttatttgtc ccttatctgc tggcagtgag
      301 gaaaacggaa gaatggatga tctcgcaaat gtctttttct gaggccattt tatgtttctt
      361 ctgtacttct atgttcctgt atatgaagag attgtatttc gagggtgttt ttttaactct
      421 ttataattct ggtttaatga taatatttac atctctgcca ttgtaacatc agtcatattc
      481 tctgcattgc atctacaata tactgacttt cgtacatttt ttatgctctt tttagtatcg
      541 ttagttctta ttagcgcaca aataaagtcg aatggtctgt taatgccata ttacttcaca
      601 tggcgatgaa tgcagtcata gcaggaatac aatatctagc ttatatcagt tattcccatt
      661 aacagagcat tttctgttac aatctcatat gataaaaatt aaacggactg gcattgttac
      721 tatgcggtat gcataaaatg ataaacgttc ggaggagcct aacttaaacc attacatagg
      781 catcattaac aatataaggt ttaatttcca ctccccgcag acgaagaagc acttgatgta
      841 catctgccgg atccaaactg ggatgataat cctatacctg agggagacat ggagggacta
      901 accgttgcag tcgttgccca tgaatgtact ttatcaccta tataagcccc tgcaatagaa
      961 ccaaatcccg gactaataaa cgtaccagca actgaaccaa tagaaattaa ttctgcctgc
     1021 ccgtcattcc cgtcacctcc agatatttgt tttatttcat tttcagatag ttttctcatt
     1081 tcaacacctt cgctataaga tttgtgaaga agtaaaaatt tacgt
//
LOCUS       DAZFFG010000324         1113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFFG010000324 DAZFFG010000000
VERSION     DAZFFG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.327"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..1052)
                     /locus_tag="ACHJ7J_005143"
     CDS             complement(<1..1052)
                     /locus_tag="ACHJ7J_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO5003209.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALAIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPL
                     ISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLW
                     LEDVENAGQPVPDEQFS"
BASE COUNT          361 a          200 c          178 g          374 t
ORIGIN      
        1 gagaactgtt catctggaac aggttgtccg gcattctcca catcttcaag ccacagcaga
       61 agacgtaata tttcatctat attttcgaag ctaacataat cgtagcgacg atgggtagca
      121 taaatttttc gtgctaattt tttatctgtg ataattggta ttccgatttc ctttgcatat
      181 ttccttacgg ctaaggctac ctcattagtt tctcttacag aaatcaatgg aataggtgaa
      241 agatgtggct taaagtatat ccctattgca atgtgagtag ggttggcaat catcaaacga
      301 ctattactga catcagattt caattgctca gaaagaattt cttgatggcg ctctctgcgt
      361 ttagacttaa tttctggatt tccttcttgt tccttgtatt ctcttttaac ttcttgttta
      421 tccattttca tatctttcat aaataaaaaa tattcagcga taaaatcaaa aattaagaca
      481 attatcatac tgccgagaca atacagtatg agaaggaata gcatctctcc ccaatctgat
      541 aataaagata tgatatctcc atcaagagtt ttaaaaagca gtgatttatg attactccaa
      601 aatactttga tcgccaatgc aaaaaaaata atataaagaa ttgctttaac aaattctttt
      661 acggttttta agccaaatat acgctttaag ccatttactg gattcaatga atcaaactta
      721 aatttaacag cttcagttgc taattgcatt ttactttgaa accaggagac aagtattgtt
      781 gtgaacatgc aaaccagcaa aaaaggaatg atcgttttca accaaccaat ccccatagct
      841 ttaaaatagt cccaaatatc tggtttagcg ttatgatcaa ggatgtattc aagaatcgac
      901 ataatgtgat gcacatcaaa aacatatccg agatataaag tacccacaag cattattaca
      961 gagacggtta agtctctact ttttagaatt tgcccttttt tagaagcatc ctggagtttc
     1021 ttttgtgttg gcttttcagt tttattggcc atgaaacact ctatttctca atgtatagtt
     1081 gcaattgttc tgggaaaaag gataacggca tta
//
LOCUS       DAZFFG010000325         1112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFFG010000325 DAZFFG010000000
VERSION     DAZFFG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.328"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..614
                     /locus_tag="ACHJ7J_005144"
     CDS             <1..614
                     /locus_tag="ACHJ7J_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001664547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5003210.1"
                     /translation="KSKKISLTCVGDFRQTIYQTAITTKKPLSTIEKKQFYIDKNFEM
                     EDISLCRRSILEICELSDTVHSNMGFSPTTSNVKVNDPNIMNHIGAFAVKDSDVNNYI
                     TRFNPVLLRSKVTSGKKYAIESLQKFTYGKAKGLGFDRVLILPTKEYISFLCGERDVF
                     NDQKTESSKNKLYVALTRARYSVAIIYPDVIPKNCPLKIWTPE"
     gene            640..>1112
                     /locus_tag="ACHJ7J_005145"
     CDS             640..>1112
                     /locus_tag="ACHJ7J_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003211.1"
                     /translation="MTNYIYSKKFWDDKDFIHHVQQENNELELLIVCDVESNFSFSSA
                     EAHRLISLGSSTVNMDSLSVAHLKINFENKKILRNIRLNEIILKSYGSRQTPGPEKYQ
                     LQIAYSLFNCQGKITSIIKKNETTDSETKTLMTSIKIKEIIGQFSEDLIELIKIV"
BASE COUNT          407 a          180 c          189 g          336 t
ORIGIN      
        1 caaagtcaaa aaaaatatct cttacatgcg taggtgattt tcgccaaaca atttaccaaa
       61 cagcaataac gactaaaaag cccttatcga cgatagaaaa aaaacagttt tacattgata
      121 agaattttga aatggaagac atttcattgt gtagacgttc aatattagaa atttgtgagt
      181 tatcagatac ggtgcattcc aatatgggtt tttcccccac cacttctaat gttaaagtca
      241 atgaccctaa cattatgaat catattggag ctttcgctgt gaaagactca gatgttaata
      301 attacattac caggtttaac cctgttttat taagatcaaa agtaacaagc ggcaagaaat
      361 atgcgattga atcattacag aaattcacat atggcaaagc taaggggctt ggtttcgacc
      421 gagtgttaat tcttcctact aaagagtaca ttagctttct ttgtggagaa agggatgttt
      481 ttaacgatca aaaaacagaa tcgtccaaaa acaaattata tgtagcctta acacgagcac
      541 gttatagtgt tgcaataatt tatcctgatg ttattcctaa aaactgtccg cttaagatat
      601 ggactccaga ataatttata aagtttattg aggattaaaa tgacgaatta tatatattcg
      661 aagaaatttt gggacgataa ggacttcata catcatgttc agcaagagaa caatgaattg
      721 gagttgctaa tagtttgtga tgttgagtca aacttttctt tttcctctgc cgaagcccat
      781 cgattaatat cccttggttc ttcaacagtt aatatggaca gtctttcagt agcgcattta
      841 aaaattaatt ttgaaaacaa aaaaatactt agaaatatac gcttgaacga aataatatta
      901 aagagctatg gctctcggca aacaccaggt cctgagaaat accagctaca gatagcatat
      961 tctcttttca attgtcaggg aaaaattaca agcataatta agaaaaatga gacaactgat
     1021 tccgaaacca aaaccctgat gaccagtatt aagataaagg aaattattgg ccaattttcg
     1081 gaggatctga ttgaattaat aaaaatagtt tt
//
LOCUS       DAZFFG010000326         1106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFFG010000326 DAZFFG010000000
VERSION     DAZFFG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.329"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(144..>1106)
                     /locus_tag="ACHJ7J_005146"
     CDS             complement(144..>1106)
                     /locus_tag="ACHJ7J_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003212.1"
                     /translation="PSSFNSNKHDIDSYFDFFDYKPDSIFSHQHFLSYILSTIKRVGS
                     LELIIFPESALSQKHFNSLKTLLFETFGENAPSLLSGVYNSNDDIGENYALLSFIEES
                     GNGFDTIRQDKHHRWCLERNQLRNYNLTSSLDPSKKWWENIAIGRRKLTTLHTNNGVS
                     LCPLICEDLARQEPVAQAVRSIGPNLVVSLLLYGPQISSRCPGKYSAVLSDDPSSSVL
                     SVTAFGMTQRSTGLGFEPSTQVALWSEPSKGSETLELPSADHAIVLELEIEQTGMWSL
                     DGRCEEKPILRKRMHSTIALDTTQSVTALKQGIAKFLNSRRSHV"
BASE COUNT          338 a          217 c          192 g          359 t
ORIGIN      
        1 aaaaatcact aattatttta agttctggga atatattaat aatgtcatgc gcatcatcga
       61 atatctctct ggcataggaa gggttttgaa tcaccatatc tgcaattgcc ctcaagagca
      121 tcttatcatc aatacttttc ttctcagaca tgtgacctcc tagaattcag aaatttagct
      181 ataccttgtt taagtgcagt cacactttgg gttgtatcaa gagctattgt ggaatgcatc
      241 ctttttctta atataggttt ttcctcacaa cggccatcca aagaccacat tcctgtttgc
      301 tctatctcca attctagcac aatagcatga tctgcagaag gcagttccaa agtttcagag
      361 cctttactag gctcactcca taaggcaact tgagtgctag gttcaaagcc tagaccagta
      421 gatctttgtg tcattccaaa tgctgtaaca gatagaaccg atgagctagg atcatctgat
      481 aatacagctg aatatttacc agggcatctc gaactaattt ggggaccata tagcagtaag
      541 ctaacaacta agtttggccc tatagaccta accgcctgag ctacaggttc ttgtctagct
      601 aagtcctcac atattaaagg acatagtgat acaccattat tagtatgcaa tgttgtaagc
      661 ttgcggcgtc ctatcgcaat attctcccac catttcttac taggatctaa acttgaggtt
      721 aagttataat tccttaattg atttcgttca agacaccagc gatggtgttt atcctgtctg
      781 attgtatcaa atccattgcc actttcttct ataaacgata aaagtgcata gttttctccg
      841 atatcatcat tactattata aactcctgat agcaatgatg gtgcattttc tccaaaggtt
      901 tcaaaaagta gcgtttttag actgttaaaa tgtttttggg ataatgcact ttctgggaat
      961 attatcaact cgagtgagcc aactctcttt attgtcgaaa gtatataaga aagaaaatgc
     1021 tgatgactaa aaattgaatc tggtttgtaa tcgaaaaaat cgaaataact gtctatatca
     1081 tgcttgttag aattaaatga tgaagg
//
LOCUS       DAZFFG010000327         1097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFFG010000327 DAZFFG010000000
VERSION     DAZFFG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.330"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            101..199
                     /locus_tag="ACHJ7J_005147"
                     /pseudo
     CDS             101..199
                     /locus_tag="ACHJ7J_005147"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            437..1045
                     /locus_tag="ACHJ7J_005148"
                     /pseudo
     CDS             437..1045
                     /locus_tag="ACHJ7J_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          331 a          278 c          245 g          243 t
ORIGIN      
        1 acctaaaatt cctgagggga tagtgtacac caaatattgt gtccactatc catgaaatca
       61 ttaatcgcag tgcgtaaaaa gcccttattt tcccgttgag ttcataatca aaatggttgt
      121 actctcgtat cggccagaaa ccaccgtagc gaaacttact cgtgagcatg ggatcaacaa
      181 taatctactg ttcaggtgat accagtactg ctacaaagga aaactacgcc tccttcagca
      241 atagaaaaca acgtggcagc acagctcccc ataacactta atgccgaata gcttgtccct
      301 acaacattct cctggtcaca acttgtagct gctgaggcgc ccgaatcact caatatcagc
      361 tgtaaagcga cattccggca ccgatcactc cggctaaatg gttccgtcag ggacaaagat
      421 ctagcaggtc gctggcatca ccgatatgag aaacggcttc aacactgttc tgcaggtgga
      481 tatgtacgcc gtgttcaacg agctgtacca cgatgggcag ataacggaaa cagcctgctg
      541 ggctcacgtc gtcgcaagct ccacgatgcg cacattcgca ctccgtctgc gctgacggag
      601 gaatccctga aacagatcgg cgagttgtat gccattgagg cggaaataaa ggaggatgcc
      661 gacaaaacta aaccgtgcag aacgtcagca aaaagctaaa gtgcgactga tatccctagc
      721 aagaagctgg ctgagtaaaa agatgaaaac gttatcgcgg ctggcgaaag tgttcacgta
      781 tttactgaat cagcagccgg cgcaggctaa caatactgaa ggtgaaagat ccgaagcaaa
      841 taacaacatt gctgataatg gccagtctag gccacaaaaa ctaactattc ttcagttcgg
      901 agcacgggga aagcgatgag ctctgctgta cagtctcatc agggcgatca aactgaacga
      961 tattaatcaa cggcgcaacc tgagttatgt gcttgacgta atcgccgact atcttataca
     1021 ccgggtcact aaactactcc cctagcgcat agcactgcca actgattaac atatctccct
     1081 caatatggtt ctcgcta
//
LOCUS       DAZFFG010000328         1090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFFG010000328 DAZFFG010000000
VERSION     DAZFFG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.331"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(57..383)
                     /gene="ybfB"
                     /locus_tag="ACHJ7J_005149"
     CDS             complement(57..383)
                     /gene="ybfB"
                     /locus_tag="ACHJ7J_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO5003213.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(383..>1090)
                     /locus_tag="ACHJ7J_005150"
     CDS             complement(383..>1090)
                     /locus_tag="ACHJ7J_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5003214.1"
                     /translation="ATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNR
                     HRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGE
                     RSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRC
                     DLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKC
                     SGNNIGRFIRFVFTGVM"
BASE COUNT          328 a          256 c          184 g          322 t
ORIGIN      
        1 gtggcatacg ggatatcaat gcccaggtta catataataa gagcaattaa cttaatttat
       61 ttgtttataa tgcgcttact taatacaaac cattgaatat accctcctga cagaaaataa
      121 agccatatta ttatcgccat aatatactta ctatcaatcc ttacgccaat aatgtctgaa
      181 acagtcccta taaatattag agcaataaag aatactatgc ctgttggaaa acatattacc
      241 atcattccat aagacatgag actaattaac tcgctcacat cacgagtgga gtctaatagt
      301 gaaagtctat gcatggaaaa gaatagtatt aaaagcgata aagccagcca tatagctctg
      361 aataaaaaaa taatatattt cattacatca ctccggtgaa tacaaatcta ataaaacgtc
      421 caatgttatt tccggagcac ttcatcttac atgtttctaa agaatcatta gaccagtctt
      481 ggcagttttt aaatagccct aaatttatag gtgtgaatat atcaggcaat acagaataac
      541 gcggccttac ctctttaaac ttccctttta aacagttctc cacacaacta ttatcggttt
      601 caacgaggtc acaacggcca gcagcttctt ttgtatcatt acttgcttta cctggccccc
      661 atagaccatc tccttttgat tctccccgtt ggtcttggcc accacaagta tagtcccctt
      721 taccatctgg gacacaaaga tattgatgat agaacggatt cccccatata tctggaccgc
      781 ttctttcacc agttccgccc attgaatgca gtggcttaat gcattttagt gtttctaatc
      841 ctaatggatc tatatttgag atcggattca acggatactg ataaaaattc catcccccct
      901 tcagcccaat cggatcctga gtgatatacc tcccctgcag cgggtcataa tagcggtggc
      961 ggttgtaata caggccggac tcctcatcat actgctgacc cggcaggcgg atgagctgct
     1021 gcagctggtg cgggttctct tcattcagca ggttgcccca ttcatcgtat tctgcgcacc
     1081 attctgtagc
//
LOCUS       DAZFFG010000329         1090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFFG010000329 DAZFFG010000000
VERSION     DAZFFG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.332"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..624)
                     /gene="sctT"
                     /locus_tag="ACHJ7J_005151"
     CDS             complement(<1..624)
                     /gene="sctT"
                     /locus_tag="ACHJ7J_005151"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO5003215.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQ"
     gene            complement(626..886)
                     /locus_tag="ACHJ7J_005152"
     CDS             complement(626..886)
                     /locus_tag="ACHJ7J_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5003216.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(896..>1090)
                     /locus_tag="ACHJ7J_005153"
     CDS             complement(896..>1090)
                     /locus_tag="ACHJ7J_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5003217.1"
                     /translation="FYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMD
                     GWTMLSKGLILQYFDLSINP"
BASE COUNT          386 a          230 c          193 g          281 t
ORIGIN      
        1 ttgtgaagca taacgtgcca gcaatcccag gacagcctca ccgcccagca taacggcaat
       61 cacggggctt gcgtaaacga tagtatgagt cattatgtta ttaatatatg agaataaagg
      121 ctctaattta ggaaaattaa aattacctga aggccataaa ttataacttt gccaaagagt
      181 ttccagaata aagttcattc caccgttagt aagataaacc gcagcagaga aaaggttaaa
      241 taatcttgct aattccgagg tatcgacacc tgtagccgga tccaaagtac tacttaatgt
      301 tgcaccgcgt tggttatcga taaagctacc tatagcaagg aaaatccaaa atggggatgc
      361 taataagcat gcaataaaca gccctatgat aatttctctg gcagcgaaca aagcaatatc
      421 gatttcattt aagttcaata attcatagtg gtaatgctga actaatgcac atgataccac
      481 tattattatt ggatgacgga ctactgttgg tatattccca ctggcgaaaa atggtaacaa
      541 atagaaagtg ggtgccaatc ggcaaaaacc gagcgctgta gcagccagca gactatgcaa
      601 ctgatagagt atcgcctcac ccatgctatc ctctcgcgaa tgctaaattc agcatttcaa
      661 taccaaaaga atataatttt tcgccatacc atccgctcat taaaaaaaag caaatactta
      721 cgcataataa tttcacgcca aatggcaacg tctgttcttg caactgtgtt accgtttgaa
      781 aaagccctac taataaacca acaaatgtcg caactgcgat aggtcctgca gacataacta
      841 aaatcagata cagcgcccta ttccctgcaa aaacaatatc gtccatattt tttcctcagg
      901 gattaatgga taggtcaaaa tattgtaaaa tgagtccttt tgataacatt gtccaaccgt
      961 ccatagcgac aaacagaatt aattttatcg gtgtagatat ggttaccggg ctcatcatca
     1021 tcatacctaa cgttaacaaa acgctggata taaccagatc cactacaaca aagggtaaat
     1081 agatataaaa
//
LOCUS       DAZFFG010000330         1065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFFG010000330 DAZFFG010000000
VERSION     DAZFFG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.333"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            285..917
                     /gene="yqeH"
                     /locus_tag="ACHJ7J_005154"
     CDS             285..917
                     /gene="yqeH"
                     /locus_tag="ACHJ7J_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5003218.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          362 a          180 c          190 g          333 t
ORIGIN      
        1 tagattcgca agatatcttc tggtcattca aaaacaattt gcgatataga tattctcata
       61 ctatgagata gttatctgga ccactctcaa ccatacaaga ttaaatcagt gaaacataat
      121 catatttaat aacgagaaat gcatttttaa atgtcttaat cagaattata gcaaatacag
      181 attaatcaca atttttccca gcaaaattac aaaatcatgg tgtattgaca tcatcaataa
      241 ccgatgaaat tatgcaatta tatacggata gggaggttct taacatgggg ctttgtagtc
      301 gttataaaag tcttacatgc aatagttgct caatgcattg ccaaataatg ccagaagagt
      361 caccgcgttt gcagtattgc gcaaactcgt gtttttgtat gtggcccgaa gaaagttcat
      421 attttaatcg tggtgtggta gaaggtatct taacaaaaaa ccacaatgcg agattaagcg
      481 gatatatttt tgtcgatttt tcagtaagtt ttttacgtct attccttgaa aaagactgga
      541 ttgactatct tgcaagtact gacatgggga ttgttttagt cagcgaccgc aatatgcaat
      601 cgttagctaa ttactggcga aaacacaact ctgcaatatc tgctgtcata tataatgatg
      661 atggtcttga tgttgccaat gaaaagatca gacaactatt tattggtcgt tatttatcat
      721 ttaccagagg taacacatta actcagatgg aattcaccat aatggggtat atggtttctg
      781 gttataatcc atatcagatt gctgaagttc tggatatgga tatccgtagc atctacgcgt
      841 acaagcaacg aatcgaaaag agaatgggtg gtaaaataaa cgaattattt attcgttcac
      901 attcggtcca acattgatac tcaaaaatct gccagaatca tactctgttt atacgggcat
      961 ttattgaaat gcccgtattt tataaatagg ccgtcccctc aagagtaaac atcatagtat
     1021 ttaaaaccat cacacgaaaa attcagaagc attacgtaat atgaa
//
LOCUS       DAZFFG010000331         1060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFFG010000331 DAZFFG010000000
VERSION     DAZFFG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.334"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            127..>1060
                     /locus_tag="ACHJ7J_005155"
     CDS             127..>1060
                     /locus_tag="ACHJ7J_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003219.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKKDEESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEEGHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETNLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHIFPDESATLVLYFSNN
                     DLECFVFERDIPSQYRFFKMFTNLALVIDQLTEAN"
BASE COUNT          334 a          220 c          195 g          311 t
ORIGIN      
        1 tgattgtttc tgagtgtaca gagaccactt tcgcctgata aatgaaaaat gagcgatttt
       61 tgcaagttag tttttctata gccgagtacg ctcagcgcct cgcttgatta caatgaggtt
      121 gctgctatga gtcctgataa tatcggttcg gctgtcaatc gcttctcagg ttggccatct
      181 aatgatcctc taaaaaaaga tgaagaatca atgacatcac ccgtgactgt gcaggtaata
      241 aagatgattg ccgccatcct tgatccacat cggatagtcg ttggaattga tacactttat
      301 acaacgcagc gcgaaacatt tacctcagag agtcaacagc tcattgaatc agcattgcaa
      361 acacttcatg tgatgaattt aaataacctg gaaacaaatc gcgatgaaga aggtcactat
      421 ctggcttttg aatataacgt tgacacctct gacagaataa ccgtttctgc ttataatgaa
      481 aaaaaacctg gctgctatgg ccaatgtctt gtaatacagg atgacgaaac aaacttattt
      541 aacctgataa aaattaaaat tttcgaagcc aggcataatt atgtcgctca attgatgagc
      601 gatccggaat ttatgttcaa attcagttat agcgctcaac aaaacagatt agaacctcat
      661 cttctggttc ccgcttattt ccctctaaaa acaaacgaac cggttacgca agaggatgta
      721 ctgttacttt atcgtttctt taaaatgaat gatgatttta ataaattaac atcagatgaa
      781 tacatgagta tcctgacacc tctgatgaag tgtgagcgta gcgtccacga caacaacagg
      841 tatgtgacgg gcaaagatac ccttcttctg gactatccac cttctggaaa ccagatacat
      901 ttccatattt tccctgacga atcagcaaca ctagtccttt atttttcaaa caatgacctt
      961 gaatgtttcg tctttgaaag agatattccg agccagtatc gctttttcaa aatgtttacc
     1021 aatctggcac tggttataga tcagttaacc gaagccaata
//
LOCUS       DAZFFG010000332         1045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFFG010000332 DAZFFG010000000
VERSION     DAZFFG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.335"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(114..899)
                     /locus_tag="ACHJ7J_005156"
     CDS             complement(114..899)
                     /locus_tag="ACHJ7J_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003220.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESPIDEKKKKTQTAQSSINKNVP
                     QQLTDKDLYGNETTLVVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVATFSGIPDRQKPVTCNKDKNKDESLDVVDAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWATWSPVNYE
                     SKVIFPQIGNKNSDSLDVAEQQISQLKQKTYAAVVKELVNRYQ"
BASE COUNT          330 a          223 c          165 g          327 t
ORIGIN      
        1 tctaaaaaaa atatatatgt aaaattaatt agcagacttg aaccagagta taaatagaaa
       61 agaaaatgct ctttcttgct aagtaccatt aaaacgtctg gtataaaacc ttactactga
      121 tagcgattca ccagttcttt aaccactgct gcgtacgttt tctgcttaag ttgtgaaatc
      181 tgctgttctg ccacatcaag gctgtcagaa tttttattcc ctatctgcgg aaaaatcact
      241 ttcgattcat aattcacagg cgaccaggta gcccactcac ctgtagccac atccaccagc
      301 gtgaatcgaa ccaaataacg cagtgacatc gtgtcagtca atttttgatt tttataatct
      361 gaccaaacta ttgatttcaa tccagagtca tattttcctg tctgcaacgt atcctggaaa
      421 acaataatcg ctttctgatg gccttttgcc gcaacaaaac gtaatgcctg catccagttc
      481 atattttcgg catctacaac atccagactt tcatctttgt ttttgtcttt attacaagtt
      541 acaggttttt gcctgtcagg aatgccagaa aatgtggcaa ctgtataata ttttctcatc
      601 tcctcctgca taatggtttc cggtgcgcga ctgccagact gaacaagtat aacgggagaa
      661 ttaagaggca cacgaaactc atctccttca agcgcagctt gaatatcctc ctcggataca
      721 accagtgtgg tttcattacc atataaatct ttgtctgtta gttgctgggg aacgttttta
      781 ttaatagaac tttgtgcggt ttgagttttt ttcttttttt catcaatagg agattcagag
      841 gcacatcctg caagcagcga gaataataca catatacaac aaacccttct taaattcata
      901 atcggacact ccctcgcctg tcataatcat ggtaatgttt agctaacgat ttttaattat
      961 atactccact tgaatcagaa caaacaaaaa taacagcaaa tataaattca ttaaatcaat
     1021 agaaaaatga aaatcataac atgca
//
LOCUS       DAZFFG010000333         1021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFFG010000333 DAZFFG010000000
VERSION     DAZFFG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.336"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>1021
                     /locus_tag="ACHJ7J_005157"
     rRNA            <1..>1021
                     /locus_tag="ACHJ7J_005157"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          247 a          232 c          320 g          222 t
ORIGIN      
        1 cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca aacacgaaag
       61 tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg ggtcagcgca
      121 agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg gtcctaaggt
      181 agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga tggccaggct
      241 gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt acccgcggca
      301 agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga gccttgatgt
      361 gtaggatagg tgggaggctt agaagtgtgg acgccagtct gcatggagcc gaccttgaaa
      421 taccaccctt taatgtttga tgttctaacg tggacccgtg atccgggttg cggacagtgt
      481 ctggtgggta gtttgactgg ggcggtctcc tcctaaagag taacggagga gcacgaaggt
      541 tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg cataagccag cttgactgcg
      601 agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg atccggtggt tctgaatgga
      661 agggccatcg ctcaacggat aaaaggtact ccggggataa caggctgata ccgcccaaga
      721 gttcatatcg acggcggtgt ttggcacctc gatgtcggct catcacatcc tggggctgaa
      781 gtaggtccca agggtatggc tgttcgccat ttaaagtggt acgcgagctg ggtttagaac
      841 gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg agaactgagg ggggctgctc
      901 ctagtacgag aggaccggag tggacgcatc actggtgttc gggttgtcat gccaatggca
      961 ctgcccggta gctaaatgcg gaagagataa gtgctgaaag catctaagca cgaaacttgc
     1021 c
//
LOCUS       DAZFFG010000334         1018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFFG010000334 DAZFFG010000000
VERSION     DAZFFG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.337"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>1018
                     /locus_tag="ACHJ7J_005158"
     CDS             <1..>1018
                     /locus_tag="ACHJ7J_005158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003221.1"
                     /translation="NVDGKNSKFIFTPQKTFYIGLNRSKGFVNITNGANFLSNGNISM
                     GSMMFNGTGGEVNVSGTGSLLKAKNFYLENSSILSITDGGRVDVENIYIGPNITPGGI
                     IISGKDASLNVAEDILWGNGGNHAICTADVYMCAFTDGNKITVRDNAVINADSQTITI
                     GNGNNSNALIAIGAAPDESPEIAGYLNARRIVFDSDNRPTLLALNYTGSNYNLYQNLE
                     STLQYNYTNDSFIPVIAFLTGTTDVSGDNSGFGGRFYIADGAEINTTVPNNLGNAVIA
                     TDGNVNIKYNGDWIFNNVLYGHGTISINTDGHTFSFLKGTLTQHQFHDSIKFTGKLAL
                     YNTDF"
BASE COUNT          339 a          175 c          213 g          291 t
ORIGIN      
        1 gaatgttgat ggaaaaaaca gtaaatttat atttacccca caaaaaacat tttatattgg
       61 cctcaatagg agtaaaggtt ttgttaatat aacaaacggt gctaattttt tatcaaatgg
      121 aaatatcagt atggggagca tgatgttcaa tggcaccggt ggtgaggtta atgtatcagg
      181 aacgggaagc cttcttaagg caaaaaactt ttatttagaa aacagtagta tattgagcat
      241 caccgacgga ggacgtgttg atgtagaaaa tatatatata ggaccaaaca taacccccgg
      301 aggcattatt atttccggga aagatgcgtc attgaatgta gccgaggata ttctatgggg
      361 aaatggaggt aatcatgcta tttgcacagc ggacgtttat atgtgtgcct ttacagatgg
      421 taataaaatt accgtccgcg ataatgcagt aattaatgca gacagtcaaa ctataactat
      481 tggcaatggc aataatagca atgcactaat tgcaataggt gccgctcccg atgaatctcc
      541 agagattgcc ggatacttaa atgcaagacg catagtattt gatagcgaca atagacccac
      601 actgctggca ctgaactata ctggaagcaa ctataatctg taccagaatc tggagagtac
      661 gcttcaatat aactacacca atgactcttt tattccggtg attgcatttc ttacaggaac
      721 tacggatgta agtggagata actccggatt tggtggtaga ttttatattg ctgacggtgc
      781 agagattaat acaactgtac ctaataatct gggtaatgcg gttatagcta cagacggcaa
      841 cgttaatata aaatataatg gagactggat tttcaacaat gttctttatg gtcacggaac
      901 cattagtata aatacggatg gacatacatt ctcttttctg aaagggaccc ttactcaaca
      961 ccagtttcat gattcgataa aattcacagg aaagcttgct ctttataaca ccgatttc
//
LOCUS       DAZFFG010000335         1015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFFG010000335 DAZFFG010000000
VERSION     DAZFFG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.338"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..701
                     /locus_tag="ACHJ7J_005159"
     CDS             <1..701
                     /locus_tag="ACHJ7J_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterotoxin A family protein"
                     /protein_id="HFO5003222.1"
                     /translation="SNDLFRADTRAPNQLRRVNGLLPRGQDDAYERGTPININLFDHV
                     MGMPTGNTRYDDGYVSTTTTLNEAHNIGQNMFGGSNEYYIYVISPAPNMFDVNGVLGR
                     YSPHPYENEVAALGGIPFSQIIGWYRVNFGVIETPMVRNRAYRSDLFHGLNIAPNEDG
                     YRLAGFPDGHQAWREEPWRRYAPLQCSTDLRVISIITCHSIINGLSNKKLSDLKKKLL
                     SVMALPVLLLIDDF"
BASE COUNT          292 a          133 c          201 g          389 t
ORIGIN      
        1 attcaaatga tctttttcgt gctgatacta gagctccaaa tcaacttagg agagttaatg
       61 gtttgttacc aagaggtcag gatgatgcat atgaacgtgg tacgcctata aatattaatc
      121 tattcgatca tgttatggga atgcctactg gcaatacgag atatgatgat ggatatgtat
      181 caactacaac tacattaaat gaggcgcata atattggtca aaatatgttt ggtggcagta
      241 atgagtatta tatatatgtt atttcacctg caccaaacat gtttgatgtt aatggtgttc
      301 ttggtcgtta cagtcctcat ccatatgaaa atgaagttgc tgcacttggt ggaatacctt
      361 tctctcaaat aattggttgg tatcgtgtta actttggagt tattgaaact cctatggtca
      421 gaaacagagc atacagaagt gatctgtttc atggtttgaa tatagctcct aatgaagatg
      481 gttatcgtct tgctggtttt cctgatggac atcaggcatg gagagaagaa ccatggagga
      541 gatatgctcc attacaatgt tccactgatt tgagagtaat tagtataatc acatgtcatt
      601 caataataaa tggcctttct aacaaaaaat tgtctgattt gaaaaagaaa cttcttagtg
      661 taatggcatt accagtattg ttgttaattg atgattttta atttttgttg tgtttttatt
      721 atatggattg gtaacttttt atattgttta tttctactgt attattttgt gaaaataatt
      781 atttttcttg ttggctgttt gtcattgatg ttgcttggta tttttatgta tatgtctgta
      841 tgttaagtta tattgtttaa attacgtatg atttttctta atatattaat ttgtttttat
      901 atcaggagaa attttatatc tgaaaccaag tcccatattt ttgcagaggt gaggttgtga
      961 gttcgataga taagatttca actggtattt cctatggcac ctccgtaggc agtgc
//
LOCUS       DAZFFG010000336         1003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFFG010000336 DAZFFG010000000
VERSION     DAZFFG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.339"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..965
                     /locus_tag="ACHJ7J_005160"
     CDS             <1..965
                     /locus_tag="ACHJ7J_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO5003223.1"
                     /translation="KQYGDFENGIPVHDTIARVVSCISPTKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAMSEKSHGREKIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          319 a          184 c          245 g          255 t
ORIGIN      
        1 tgaagcaata tggtgatttt gaaaatggta ttcctgttca cgataccatt gccagagttg
       61 tatcctgtat cagccctaca aaatttcatg agtgctttat taactggatg cgtgattgtc
      121 attcttcaga tgataaagac gtcattgcaa ttgatggaaa aacgctccgg cattcttatg
      181 acaagagtcg ccgcagggga gcgattcatg tcattagtgc gttctcaaca atgcacagtc
      241 tggtcatcgg acagatcaag acggatgaga aatctaatga gattacagct atccctgaac
      301 ttcttaacat gctggatatt aaagggaaaa ttatcacaac tgatgcgatg ggttgccaga
      361 aagatatcgc agagaagata caaaaacagg gcggtgatta tttattcgcg gtaaaaggta
      421 accaggggcg gcttaataaa gcctttgagg aaaaatttcc gctgaaagaa ttaaataatc
      481 cagagcatga cagttacgca atgagtgaaa agagtcacgg cagagaaaaa atccgtcttc
      541 atattgtttg cgatgtccct gatgaactta ttgatttcac gtttgaatgg aaagggctga
      601 agaaattatg cgtggcagtc tcctttcggt ccataatagc agagcaaaag aaagagccag
      661 aaatgacggt cagatattat atcagttctg ctgatttaac cgctgagaaa ttcgccacag
      721 cgatccgaaa tcactggcac gtggagaata agctgcactg gcgtctggac gtggtaatga
      781 atgaagacga ctgcaaaata agaagaggaa atgcagcaga attattttca gggatacggc
      841 acattgctat taatattttg acgaatgata aggtattcaa ggcagggtta agacgtaaga
      901 tgcgaaaagc agccatggac agaaactatc tggcgtcagt ccttgcgggg agcgggcttt
      961 cgtaatcttg ccctggcttc acgccactca tcagggttga aac
//
LOCUS       DAZFFG010000337          978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFFG010000337 DAZFFG010000000
VERSION     DAZFFG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.340"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(91..>978)
                     /locus_tag="ACHJ7J_005161"
     CDS             complement(91..>978)
                     /locus_tag="ACHJ7J_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5003224.1"
                     /translation="LFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYVNLDPVIQPG
                     QNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNSVTYPFPLTT
                     NTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNFT
                     WNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTD
                     SARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKLPLGLSATYGQTG
                     NKVSAGTVQSVIGVTFIYE"
BASE COUNT          275 a          224 c          208 g          271 t
ORIGIN      
        1 ttcttacagg tgtaggataa tagccttcac aaaataatta tccgcgcaat tttcgtgaat
       61 ggagcgcata gactaaggtc aaaatcacaa ttattcatag ataaaagtga caccaatgac
      121 tgactggacg gttccggctg aaaccttgtt tcctgtttgt ccataggttg cgcttaaacc
      181 cagaggtagt ttgcttttat ttactgtccc cagggagaca ttttctcctg tagccagtat
      241 ttttccatta cgcatcagag agacacccac tcccgatgct tttgttgcat ctggtgctgt
      301 gttagcaaac acttgtcgag cactgtcagt ggtggcaccc gaaagataaa atgagagctt
      361 ttgttcactt gagcaatata cgccaagggg gatttctgcg cttccaggaa agtccgggag
      421 attcacggtg acattacgac tatcaacagt gcagccgcct gttggcataa caacactgtt
      481 atttgaaata atattccacg taaaattacg cggatttccg cttcctaacg tggcaatttt
      541 atacatatga atacgggcaa taacttcccc ggcttttatg acaactccac cggccgcccc
      601 gactggggta atatagagtt ttaacggtaa tggcatcggt gtcttatcac caatatccaa
      661 tacgttggta tttgtcgtca acgggaaggg gtaggtcaca ctattccagt acagcgatcc
      721 tttatatgac tgtagggagc ctgcgaaagc tgagccttgt accaggttta tatgatcagt
      781 atcgtaccag ccaccgtaat cattccagca acttatatgc tgagacaagt ctacaaccag
      841 attctgtccc ggttgtatta cagggtcgag attaacataa acagaggttg tacctgctcc
      901 aatacttgac ccgccatcga cattacaact gaaggcaaaa actttgcctg ccattaatag
      961 aagataaata ccgaacag
//
LOCUS       DAZFFG010000338          961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFFG010000338 DAZFFG010000000
VERSION     DAZFFG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.341"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            21..782
                     /gene="ydeO"
                     /locus_tag="ACHJ7J_005162"
     CDS             21..782
                     /gene="ydeO"
                     /locus_tag="ACHJ7J_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO5003225.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          292 a          183 c          187 g          299 t
ORIGIN      
        1 tattgtttta aggagataaa atgtcgctcg tttgttctgt tatatttatt catcatgcct
       61 tcaacgctaa cattttagat aaagattacg ccttctctga cggcgagatc ctgatggtag
      121 ataacgctgt tcgtacgcat tttgaacctt atgagcggca ttttaaagaa atcggattta
      181 atgaaaatac cattaaaaaa tatctacaat gcactaacat ccagacagtg acggtgcctg
      241 ttcctgcgaa gtttttacgt gcttctaatg taccgactgg attgcttaat gaaatgattg
      301 cttatctcaa ctcggaagaa cgcaatcatc ataatttttc agaacttttg cttttttctt
      361 gcctgtctat ttttgccaca tgcaaaggtt tcattacact attaactaac ggtgtgctat
      421 ccgtttcggg gaaagtgaga aatattgtca acatgaagct ggcgcaccca tggaagctga
      481 aagatatttg tgactgcctg tacatcagtg aaagcctgtt gaagaaaaaa ctaaagcaag
      541 agcaaacgac attctcacag attctattag atgccagaat gcagcacgca aaaaatttga
      601 tacgcgtaga aggttcagtc aataaaattg ccgaacaatg tggttatgcc agtacatctt
      661 attttattta tgcgttccgc aaacatttcg gcaacagtcc gaagagagtt tctaaggagt
      721 atcgttgtca aaggcacacg ggtatgaaca cgggcaacac gatgaatgct ttagctattt
      781 gattatttgc taacgagtag tcaaccacac acgctacgta agaattaaat ggggcagcca
      841 ttccctgccc cgcgttgttt ttaggcgata gatttattga aataaataag tgacattcat
      901 cacatattta tgcatttgca taacctgttg catgattatt tatgatctca attcggcatt
      961 t
//
LOCUS       DAZFFG010000339          960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFFG010000339 DAZFFG010000000
VERSION     DAZFFG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.342"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..215
                     /locus_tag="ACHJ7J_005163"
     CDS             <1..215
                     /locus_tag="ACHJ7J_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO5003226.1"
                     /translation="SVQYENISVVLSKRRDIIEQAPTYEISEPVFAYEKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            231..812
                     /locus_tag="ACHJ7J_005164"
     CDS             231..812
                     /locus_tag="ACHJ7J_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO5003227.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNITPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLICLLSGCHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            754..>960
                     /locus_tag="ACHJ7J_005165"
     CDS             754..>960
                     /locus_tag="ACHJ7J_005165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003228.1"
                     /translation="MRKKIEMSLIKSPANGVVIKRKISDGLKEIVSLKENILLETTEK
                     IQSIEVKREEKFIQGYYDGYTKGII"
BASE COUNT          323 a          158 c          167 g          312 t
ORIGIN      
        1 caagcgtaca atatgaaaat atttctgttg tattgtcaaa acgaagagac attattgagc
       61 aagcaccaac atatgaaatt agcgaacctg tttttgctta tgaaaaggct atgccggttt
      121 ctatcttact tgcattaata tccgtagcga cttgctggct gttatggaag tatagggcca
      181 ttctaactaa tttagtcagg ttgaaaatta agtagacata tgagagcctt atgaatcttg
      241 ccctaagaaa gattatatat gatccaatta gttatattca tcctcaacga gtaagcttga
      301 atattacgcc tattaacaat ccggtgctta gatctattac caatgaaatg attcttctgc
      361 agtacaatct ttcagttgaa cattttaatc tgaatagttc acttatttac tacataaaca
      421 attggaattt atttccattg atctgtttac tcagtggttg tcacttttat cgagaaaggt
      481 ttgcagaacg cggatttttt tataaggttc ctgctgtgct aagggattat ttatcagcta
      541 taccggtgga aataaatgaa aaggcacgat acaaacccgg catcgctaac tatcacaata
      601 tcatcacatg tggtttttca acgttgttac cctatataag gcaacaacca ttggcaatgc
      661 agcaacgatt taatttatta tttcctgatt ttgttgacca catacaattg ccgttaccac
      721 ttgcgtcaac tcttttagag agaattacat tttatgcgaa aaaaaataga gatgagcttg
      781 ataaaatctc ctgcaaatgg tgttgtgatt aaaagaaaaa tttctgatgg attgaaagaa
      841 atagtttctc ttaaagaaaa catccttctt gaaaccaccg aaaaaattca gagcattgag
      901 gtaaagagag aagaaaaatt tattcaaggt tattatgatg gctatacaaa aggcattatt
//
LOCUS       DAZFFG010000340          956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFFG010000340 DAZFFG010000000
VERSION     DAZFFG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.343"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..588)
                     /locus_tag="ACHJ7J_005166"
     CDS             complement(<1..588)
                     /locus_tag="ACHJ7J_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003229.1"
                     /translation="MDNLIGTPPNHAVPHNYIDMEQMEYLCHLNRFSKLSDDFLINGP
                     REDLFTYKYVLDGSFSNLQHLLPKGPLQKIQQRLSSLMHKNMFHCFHVFLLKLCSIES
                     IPLPNADYAFFDGEMPFTLTDEQIENISFLNAYHKEKKGNNDIVTFDFMSADHHYNFS
                     TTIALTNDSFHISSINNHNSQVIFDENIHLHPYELP"
BASE COUNT          325 a          141 c          170 g          320 t
ORIGIN      
        1 tggtaattca tagggatgta aatggatgtt ttcatcgaat ataacctgtg agttatggtt
       61 gtttattgaa gatatatgga agctatcatt tgttaaagct atggttgtgc taaaattata
      121 gtggtgatca gcgctcatga aatcaaatgt aacaatgtcg ttgtttcctt tcttttcttt
      181 gtgatatgcg tttaaaaaag aaatattttc gatctgttca tcagttaatg taaatggcat
      241 ttcgccatca aagaaagcgt aatcggcatt tgggagagga atactctcaa ttgagcatag
      301 ttttaataaa aaaacatgaa aacaatgaaa catgttttta tgcataagag aacttaatct
      361 ctgttgtatc ttctgtagtg gtcctttagg aagtaaatgc tgtaaatttg aaaatgaacc
      421 atctaaaaca tatttatatg taaaaaggtc ctctcttgga ccatttatta aaaaatcgtc
      481 agacaattta gaaaaacgat tgagatgaca taaatattcc atttgctcca tgtctatata
      541 gttatgggga actgcgtggt ttggtggtgt gccaataaga ttatccataa atcaccttga
      601 taaaatttaa taaaatctct ttattttaac ctcgacaata aaagcggcta taaaaaaacg
      661 ctcattttct gatgctgcgg ttaacccgtg gaatgtaccg ttaaccccct aaaaattgca
      721 aatccagggt gaacaccctg ggttaaaatg gaaataatga gctatttttt acagtattca
      781 ccggtaatat aagtaacact tgttgctaac ctaactagca gtacggttaa ttaacaacat
      841 agaagttatt gtttttcaaa caactcaata ttgcacgccg gagggtttat ggttgctaaa
      901 ttaaaaccaa gtgttcttat taatactaat ccttttttag aatcaatgaa taagga
//
LOCUS       DAZFFG010000341          943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFFG010000341 DAZFFG010000000
VERSION     DAZFFG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.344"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            308..383
                     /locus_tag="ACHJ7J_005167"
     tRNA            308..383
                     /locus_tag="ACHJ7J_005167"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:341..343,aa:Ile,seq:cat)
BASE COUNT          292 a          164 c          200 g          287 t
ORIGIN      
        1 tatggagtga aaagtaacag atatcataat gatgcgaaaa ctcttcagtt aatgtaataa
       61 acttatatta ttagatacaa aatcattaca aagtatcatt gaatcgatga taactataga
      121 tagtgttaat tattaacatt tgcttgttga ctgcgagtat cgactttgcg aatggaggcg
      181 atgttgggtt ttatgtcgca tgtattgacg acccaggatg gatgagaacg cataaaaatc
      241 tgttatttac atcataagat catcatgttt attgcgtggt ttataagtat tgttattgca
      301 tacttaaggc cctttagctc agtggttaga gcaggcgact cataatcgct tggtcgctgg
      361 ttcaagtcca gcaggggcca ccaaatcatc attcatgtat caggaagagt taagggtaca
      421 aggggtactc gatggcggtc tatatttcat gactaacgcc atttaaagga agaagtgcgg
      481 tgggtggttt cgaatgaggg ctggtgtttt ggtacgtggc acgccactgc aatgtattta
      541 tccatcagta ctcattcaaa agtacctgga aaaaatgccc gctatgccaa gcgggcaaat
      601 tgcttacatg tggaattaag taattaacga taaagccaga gtattatcat ttattatacc
      661 cattcattta atcaggttaa ttactcaaat cactgaacta ttctctgttc gctcactgat
      721 gatgagtgag taccacaacc aatgtatgta gaacaatgcc gtagagtaag gatgaaatgt
      781 ctctgccacg ccttaaacat ctactactga ctagaacacc cgccgcaggt agatgagttt
      841 tgtgagcgtt acaggagtct ggctttgctc attaatcaac gctatttaga tataaatttc
      901 taacactcga aaaacgattt tcactaatta attataacgg gtg
//
LOCUS       DAZFFG010000342          938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFFG010000342 DAZFFG010000000
VERSION     DAZFFG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.345"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            16..>938
                     /locus_tag="ACHJ7J_005168"
     CDS             16..>938
                     /locus_tag="ACHJ7J_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO5003230.1"
                     /translation="MNRIYSLKYCHLTQGLIAVSELASRVSSKTGRKLLATFIVSALS
                     YGAAEYACAAQMDTRNFWIRDYLDLAQNKGAFQPGAYGVKTPLKNGGEFSFPEVTIPD
                     FSPVSAKGATTAIGNAYSVTASHNGTIHHAIKTQTWGQSDYHYVDRVTKGDFAVQRLA
                     KFVVETAGATEHVDFGLSAAEALERYGIEFNGKKQIIGFRVGAGATGVTSYGVGQAYN
                     PLLRSASMFQLNWNNMLATNNTGGFYNEVTGGDSGSGFYLYDNQKKKWVILGTLTGKV
                     FSSKDTWAFFARYDQNTVDTLKNTFTQEVNL"
BASE COUNT          271 a          182 c          218 g          267 t
ORIGIN      
        1 tatggagtct tacccgtgaa cagaatatat tcacttaagt attgtcacct tactcaaggg
       61 cttatagctg tatcagaatt agcatcccga gtatcctcta aaaccggtcg aaaacttctc
      121 gctacattta tagtttctgc cttgtcttat ggtgcggctg aatatgcctg tgcagcacag
      181 atggacaccc gaaatttctg gatccgtgat tatcttgatt tggcacagaa taaaggtgca
      241 tttcagcccg gagcatacgg ggtaaaaacg ccattaaaaa atgggggaga attcagtttt
      301 cctgaagtaa cgatccctga tttttctcca gtatccgcta aaggtgcaac aactgctatt
      361 ggtaatgcct acagcgttac agcaagtcat aatggcacta ttcaccatgc cattaaaact
      421 cagacatggg gacagtcaga ttatcattat gttgatcgtg tgaccaaagg tgactttgcg
      481 gtccagcggc tggctaagtt tgttgttgaa actgcaggtg caacggaaca cgttgatttt
      541 ggtttatctg cagcagaggc acttgaacgc tatggcattg agtttaatgg gaaaaaacaa
      601 ataatcggtt tccgggtcgg agccggagca actggtgtta catcttatgg ggtgggacag
      661 gcatataatc cattattacg cagtgcctct atgtttcagt taaactggaa caatatgtta
      721 gccacgaata atacaggtgg gttttataat gaagtgacag gaggtgacag cggttccgga
      781 ttttacttgt atgataatca gaaaaaaaaa tgggtcattt tggggacact gacaggtaaa
      841 gtattctcca gtaaggatac ctgggccttt tttgctcgat atgatcagaa cactgtcgac
      901 accctgaaaa atacttttac tcaggaagtg aatctcaa
//
LOCUS       DAZFFG010000343          932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFFG010000343 DAZFFG010000000
VERSION     DAZFFG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.346"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>932)
                     /locus_tag="ACHJ7J_005169"
     CDS             complement(<1..>932)
                     /locus_tag="ACHJ7J_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003231.1"
                     /translation="QNNKLQPAEIYKESQQTAKLEFFCDEFLKISQSRYGLSTSTDSI
                     ANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKNEFAADYEPYTLTFT
                     RGENNEGILSVESKEGFISQRTINLNEYETAINIINEHVIKENIHNTVQSPTEKDISK
                     INSSDKPYKTSSKESIKSQLYSDQKNIQTCYYILRKNTEWYKYASSEERYDKFKNSSK
                     EIKNTYKQIVQAQKKLNQMKYINKLSGELIDITDKKLAPLINDSFSYTRDFFAYSKQE
                     KNIFTFDNSKFVDPKEKEGLMVQHSNGQLVITGK"
BASE COUNT          237 a          143 c          164 g          388 t
ORIGIN      
        1 atttaccagt aataaccagt tgcccattgc tgtgttgtac cattaaacct tctttttcct
       61 ttggatctac aaacttagaa ttatcaaatg tgaagatatt tttttcctgt tttgagtaag
      121 caaagaaatc tcgtgtatag ctgaaactat catttattaa tggtgctaac tttttgtcgg
      181 tgatatcgat aagctcacca cttaatttat tgatgtattt catttggttc aattttttct
      241 gcgcttgtac aatttgcttg taggtatttt taatttcctt agaagaattc ttgaatttat
      301 catagcgttc ctcggaggat gcgtatttgt accattctgt attttttctc agaatgtagt
      361 agcaggtctg tatatttttt tgatcgctgt aaagttgact tttaatactt tccttactgc
      421 tggttttata aggtttatca gagctgttta tttttgaaat gtctttttct gtcggtgact
      481 ggaccgtgtt gtggatattt tcttttataa catgttcatt gatgatgtta attgcagttt
      541 catattcatt taagtttatt gttctttgtg aaataaaccc ttctttgctt tcgacactca
      601 aaataccttc gttgttttca cctcgggtaa aggtaagggt atagggttca taatccgctg
      661 caaattcatt tttatacgtt atgacatgtt cgacaaaatg tcttattggt acgaaaccat
      721 aactgtgaac gtcaattttt tgggtcttga tactatcaat tgcatctgag gctttcgtga
      781 aaaaagccaa taaattagct atgctgtctg ttgaggttga taacccgtat ctactttgag
      841 atatttttag aaactcatcg caaaaaaact caagtttggc ggtttgttgt gattctttgt
      901 aaatttctgc tggttgcaat ttattatttt gt
//
LOCUS       DAZFFG010000344          913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFFG010000344 DAZFFG010000000
VERSION     DAZFFG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.347"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..754
                     /locus_tag="ACHJ7J_005170"
     CDS             <1..754
                     /locus_tag="ACHJ7J_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003232.1"
                     /translation="TFYSGFSNNEKKSNFFKRHGYKKMDGSSIRFNSHQIRHLLNTLA
                     QRQGLTDEEIAKWSGRVNVKQNRVYNHISEEEILEKYESLKEQSENFIITEKGDFKDK
                     NYIHGSIPISGNSLHITEFGYCTHDYVISSCEKFRDCVNCSEQLCIKGNSENLTRLKS
                     RLAEISNLIDKVSDRMNGDIQQMKNDQWLMVHTRTKERLIELIGILENEKIPDGSFIR
                     LDNTNFTHLSRIIGEQRLLKRNMENINVKKTN"
BASE COUNT          362 a          123 c          177 g          251 t
ORIGIN      
        1 gacattttat tctggtttta gtaataatga gaaaaagtct aattttttca aaaggcatgg
       61 atataagaaa atggatggaa gtagcatcag atttaattct catcaaataa ggcatctact
      121 caatactctt gcacagaggc agggactaac tgatgaagaa atagctaaat ggtcaggaag
      181 agtgaatgta aaacagaacc gagtttacaa ccatattagc gaggaagaaa tcttagaaaa
      241 atatgaatcg ctgaaagaac agtcagagaa cttcattata actgaaaaag gtgattttaa
      301 agataagaac tatattcatg gctcgatacc aatcagtggc aattcactgc atatcactga
      361 atttggatat tgtacacatg attatgtaat atcctcatgc gaaaagttta gagactgtgt
      421 taattgctct gagcaacttt gcataaaagg taatagtgaa aatttaacga ggctaaaaag
      481 caggcttgca gaaataagca atcttataga taaggtttca gatagaatga atggagatat
      541 tcagcaaatg aaaaatgatc aatggttaat ggttcacaca aggaccaaag aaagattaat
      601 tgaattgatt ggtatattgg aaaatgaaaa gattcctgat ggttcattta tcagattaga
      661 caacacaaat ttcacacatc tttcaaggat aataggagaa caaagacttt tgaaacgaaa
      721 tatggagaat ataaatgtca aaaaaactaa ctaagagtga aaagaatatg atacttgata
      781 caatatgggg atggagtgat tcatcaatta catgggaaca actttgtgag gaatgttctg
      841 tatttcttaa atcccgaccg agtaggcaaa ctctgtccta tcacaaagat attgctaatg
      901 cctacctaag taa
//
LOCUS       DAZFFG010000345          908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFFG010000345 DAZFFG010000000
VERSION     DAZFFG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.348"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>908)
                     /locus_tag="ACHJ7J_005171"
     CDS             complement(<1..>908)
                     /locus_tag="ACHJ7J_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5003233.1"
                     /translation="DNNHVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVDGFSIK
                     ERNFFTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTA
                     YFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCDLLKHEDMASFAYENKKLKKLFTLL
                     DKRDMILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNK
                     DSRINKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYNLIKTLDVCNYDSETKVLTS
                     HHGIIRDEENKCYCLGALQVPFNQMK"
BASE COUNT          294 a          180 c          134 g          300 t
ORIGIN      
        1 ttttcatctg attaaatgga acctgcaaag caccgagaca ataacattta ttttcttcat
       61 cccttattat tccgtgatga ctggttaaaa ctttcgtttc tgagtcataa ttacaaacat
      121 cgagcgtttt aattaagtta tatgtatcac cggaagagag tttatgattt gctagtcgtg
      181 cactatagtt accattaaaa ttgatttcat gatttccagc cagaacgacg acatttttat
      241 ttatacgact atctttatta ccctccatat ttcgcataga atttagcaaa gtaaggatat
      301 atttatcacc aaaaatagta ctgaatctat cgcctgtatg atccccaagg aagatacaag
      361 gggtaatcgc atcgcctttt aaaaaaacat gttgatgcaa ctcattcaag atcatgtctc
      421 tcttatctaa taaagtgaag agttttttca attttttatt ttcatatgcg aatgaagcca
      481 tgtcttcgtg tttcaataaa tcacataata aactccaccc cttttccctg atgcctatat
      541 gccctgaaac gagcaatgca taaagtacag cgcctactct tccatctgta tcgccaaaat
      601 aagccgtgtt ggcattttgt gggttaatca caccacactg tttataaaag tctatttttg
      661 aaaaaacaga ttcaacatag ctatcaaact tttccttagt aaacgtctct ttagagacag
      721 cccaacgcca gtcaggtttg aaattaccac taagaagagt aaagaaattt ctctctttga
      781 tagaaaaccc atcaacttta taaattatgt ctgatttatc cccggagaaa ttaataaaat
      841 aataaccgtc cttacgctca atcgtaacgg taatgggaga cgataatttt atcacatggt
      901 tattgtct
//
LOCUS       DAZFFG010000346          894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFFG010000346 DAZFFG010000000
VERSION     DAZFFG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.349"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..551
                     /locus_tag="ACHJ7J_005172"
     CDS             <1..551
                     /locus_tag="ACHJ7J_005172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003234.1"
                     /translation="TVGGAGVSKKNIKNAKAKMLKGVSKYWNNKFKLKLKDAICGDIE
                     LPVRFKILWVKSNEHYLMNIHKKFPREGVTGFEMNVSGSTRDWIYAHEFGHCFGLPDE
                     YAYDPAGGETVAYYKPDGTMDNKINVPYDGGITNDPSSTIMSAVDNTIVLERHCWPIA
                     IEANDIMKNSKRNITTKCEVML"
     gene            612..>894
                     /locus_tag="ACHJ7J_005173"
     CDS             612..>894
                     /locus_tag="ACHJ7J_005173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003235.1"
                     /translation="MSDLSVNDFAIWVECDNSKTSECLYTKKNNLLIDINFRNDSGRD
                     IYVPFEYLRKTGPAIKLMNFNGSDGFYLRPNLTSKELLSHLTVIHSGESV"
BASE COUNT          320 a          118 c          189 g          267 t
ORIGIN      
        1 aaactgtggg tggtgcaggt gtgagtaaaa aaaacataaa aaatgcaaaa gcgaaaatgc
       61 tcaaaggggt aagtaaatac tggaataata aattcaaatt gaaactaaaa gatgctattt
      121 gtggagatat tgagctacct gttcgattta agattttatg ggtaaaatca aacgaacatt
      181 atttaatgaa tattcacaaa aaatttccaa gagaaggtgt tactggattc gagatgaatg
      241 tatctggttc aactcgtgat tggatatatg cccatgagtt cgggcattgc tttggtttac
      301 ctgatgaata tgcttatgat ccagcaggtg gagaaacagt tgcatactat aaaccagatg
      361 gaacaatgga taataagata aatgtcccat atgatggtgg aattactaat gacccttcat
      421 caacaataat gtcggcagtg gacaacacga ttgttctgga aagacattgc tggcctattg
      481 ctatagaagc taatgatata atgaaaaatt ctaaacgcaa tataacaacg aaatgtgaag
      541 ttatgctgta gttacgacga gtgatttgtt gtactaataa tacgaaatcg aatggaaatt
      601 aggggaatac aatgagtgat cttagtgtaa acgattttgc catttgggtt gagtgtgata
      661 actcgaaaac ttctgaatgc ttgtatacta aaaaaaataa cttgttgatt gatattaatt
      721 ttcggaatga tagtggccgt gatatttatg tcccctttga gtatttaaga aaaacaggtc
      781 cagcaataaa attaatgaat tttaatggga gtgacggatt ttacctccgt ccgaatctta
      841 caagcaagga attattgtct cacttaactg taatacattc aggggaaagt gtta
//
LOCUS       DAZFFG010000347          891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFFG010000347 DAZFFG010000000
VERSION     DAZFFG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.350"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..652)
                     /locus_tag="ACHJ7J_005174"
     CDS             complement(<1..652)
                     /locus_tag="ACHJ7J_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003236.1"
                     /translation="MTSRRIAYAYFDPVWDKSVHSTAQLVRTSVGPLEYQEEMKDHLI
                     CPQCYEPLNRIPSESSITIMTDGREALFRHSPNPNAPFCLLRSGAIIGKRYSSEEEAQ
                     KAVSDNDVIIISGFMQERPELVYRDNTDSSATPTEMYFESKNGVSVEIPISRHQGETF
                     NLPSKITSVQALCTNFQENYYKEIFITNGTGHAHSYIFCDALKSISEIRSEQEEPGF"
BASE COUNT          272 a          159 c          187 g          273 t
ORIGIN      
        1 aaaaacccgg ttcttcttgt tcagagcgga tctcagaaat actttttaat gcatcgcaaa
       61 aaatataact gtgagcgtgg ccagtgccat tagtgataaa tatctcttta tagtagtttt
      121 cttgaaagtt agtacataga gcttgcacgc ttgttatctt gctgggcaga ttgaatgttt
      181 caccttgatg acgagagatg ggaatctcaa cagatacccc attcttactt tcaaagtaca
      241 tttccgttgg agtggcggaa gaatctgtat tatcacgata tacaagttct ggtcgttcct
      301 gcatgaatcc ggatatgatg atgacatcat tgtcgctaac agctttttgg gcttcttctt
      361 cactagaata gcgtttcccg ataatagctc cgcttcttaa caaacaaaaa ggagcgtttg
      421 gatttgggga gtggcggaag agagcttctc gcccgtcagt cataatcgtt atacttgact
      481 ctgatggtat acgattaagt ggctcataac attggggaca aatgagatga tctttcattt
      541 cttcttgata ttctaatggc ccaacacttg tacgaactaa ctgtgctgta ctgtgtacgc
      601 ttttatccca aacaggatcg aaataagcat aagcaatgcg gcggctagtc atgagttatc
      661 tccgataagg tagaatatat agaattcgaa caaagttttg gctgttgtat tgtttgagca
      721 attactaagc ctattttgca tagtaactaa acaaacacat ttgttacaat ctatttacat
      781 aaatttaaga ataaaaatat gtgcaaacgg ccttaattta gcttgaatac tgagggcaaa
      841 aatggggcaa aatgccgtaa aaggggcaaa aatggggcaa caaaaatgtt g
//
LOCUS       DAZFFG010000348          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFFG010000348 DAZFFG010000000
VERSION     DAZFFG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.351"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..284
                     /locus_tag="ACHJ7J_005175"
     CDS             <1..284
                     /locus_tag="ACHJ7J_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5003237.1"
                     /translation="SNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDPRKNIR
                     QKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPH"
     gene            546..>885
                     /locus_tag="ACHJ7J_005176"
     CDS             546..>885
                     /locus_tag="ACHJ7J_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003238.1"
                     /translation="MKIGTVAGTNDSITTIATNDMVQEHVTNFTKDLFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSFQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNSYQFIVTIN"
BASE COUNT          300 a          137 c          175 g          273 t
ORIGIN      
        1 gaagcaatac tatggcaccc acacctgtat atgctgatta tttaaataac actactgatt
       61 tcagaccaga tcctgaagat atcttcaaat gtggacagac gctcgaatgt ggcgaccccc
      121 gcaaaaacat tagacaaaaa tatggacatc atgggttagg ggtaaatcag aatcaacaat
      181 ttgataatgg tgttatgggg ttaaattcca ggatggcagt gtttgatgaa aataaaaaaa
      241 taataggttc ttcctcaggg cttagttgtt ttcaaccaca ttaaaactca atgagtgctg
      301 tggtatatct ttttaatgct gccagtattt cataggtttt cttaatcgat tgtagaggta
      361 tgtactgatg acgcctctac aatctttagg gactttctct actatgagat aagaaatttc
      421 atattatgct ttaaggctga atgatttgtg caaaatataa ttatttttcc atcgaacatg
      481 aggtgtttgt cataagatta aatgataact atgtattcct cgcggtgaat tatatgaggt
      541 aaaagatgaa aattggaact gtggcaggta ctaacgacag tataacgaca atcgcaacaa
      601 atgatatggt gcaagagcat gttactaact tcactaaaga cctgtttggg tatattgcaa
      661 atggtatagg tgatgatata tcaagtattg ccaggacaat gctcggtgaa gttgtggaaa
      721 aaattgatga ttggcaaatc gaacgtttcc aacaatcttt tcaagatgac aaaatatcat
      781 ttaccataca aacgaatcat tcagaaaaat atagtatgct atcgggcatg cgtgcccata
      841 ttctacgtag gaataatagt tatcagttta ttgtgacaat taata
//
LOCUS       DAZFFG010000349          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFFG010000349 DAZFFG010000000
VERSION     DAZFFG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.352"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..438
                     /locus_tag="ACHJ7J_005177"
     CDS             <1..438
                     /locus_tag="ACHJ7J_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO5003239.1"
                     /translation="SVEVIDEDPIRDLCVLKFKAGSFSGPNIILSSLDKISIGEEVGV
                     LGYPHCNEGRKVLTFQTARVGAKVLLSSNNVKSKHAVINLQSRPGQSGSVIFNLKNNE
                     VVGVLVGAYTNNAAGSVFIGGINPAALHQTTYAISAEYIKDML"
     gene            435..881
                     /locus_tag="ACHJ7J_005178"
     CDS             435..881
                     /locus_tag="ACHJ7J_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003240.1"
                     /translation="MISIPNEDAEAWLSTLKKYQSSTIKSFLEQGTEEQALESWLTAS
                     GPSLTSPFGGDASSMPGKKAFVDQFKQEFRAFVCGSDKYVKERNGLGELGGDIKTYLV
                     SVISSSIATSLGTTTVFIAPAVVIMLITISKMGLNAWCSLETVKDS"
BASE COUNT          267 a          137 c          190 g          289 t
ORIGIN      
        1 tctgttgagg taattgatga ggatccaatc agagatcttt gtgttttgaa atttaaggca
       61 ggatcgtttt ctgggcccaa tattatcctc tcaagcttag ataaaatatc tattggtgaa
      121 gaagttggtg ttttgggtta ccctcattgt aatgagggta gaaaagtact tacattccaa
      181 acagcaagag tcggtgctaa ggttttactt tcgagtaata atgtaaagtc taaacatgca
      241 gttataaatt tgcaatcaag accaggtcaa tcaggttcag ttatttttaa tttaaaaaat
      301 aatgaggttg tcggggtgtt agttggtgct tatacaaata atgcagcggg ctctgtattt
      361 attggaggaa ttaatcctgc agctttgcat caaactactt atgcgatttc agctgaatat
      421 attaaggata tgttatgatc tctataccaa atgaagatgc tgaagcttgg ctatctacct
      481 taaaaaaata tcaatcatca actattaaat cctttctcga gcaaggtacg gaagagcaag
      541 ctcttgaatc atggttaaca gcttcaggcc caagtttaac atctccattt ggaggggatg
      601 cttccagcat gccgggtaaa aaggcatttg ttgatcagtt taagcaagag tttagagctt
      661 ttgtttgtgg aagcgataaa tatgtcaaag agaggaatgg attgggtgag cttggcgggg
      721 atattaaaac ctatcttgtc agtgttattt caagttcaat tgctacctct ttaggtacta
      781 ctacagtatt tattgctccc gctgttgtaa tcatgcttat tacgatctca aaaatgggtc
      841 ttaatgcgtg gtgttcatta gaaaccgtaa aagactctta aaa
//
LOCUS       DAZFFG010000350          871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFFG010000350 DAZFFG010000000
VERSION     DAZFFG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.353"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(155..586)
                     /gene="tssE"
                     /locus_tag="ACHJ7J_005179"
     CDS             complement(155..586)
                     /gene="tssE"
                     /locus_tag="ACHJ7J_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5003241.1"
                     /translation="MAELSLYEKLYGNFSEGFEINVLCEHEQIILSILDNMQRILNTR
                     AGSLKHLPDYGLPDMANILQGMPGTAHQLMCVLSDVLLKYEPRIKRVDVTMPEQTQPG
                     ELHYFIDAELKDAGLVRYGTTFMPEGRVLLRHLKQQRYVQT"
     gene            complement(666..>871)
                     /locus_tag="ACHJ7J_005180"
     CDS             complement(666..>871)
                     /locus_tag="ACHJ7J_005180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003242.1"
                     /translation="VIKTEGNIVTFNYNQRERAGFEVRAAYSYALIYNGKGGARVKAI
                     SEDVIVSMDGDISVNDSVKLQLY"
BASE COUNT          259 a          224 c          141 g          247 t
ORIGIN      
        1 aaaaactcat acattattag tgttgatatg ccagagttat caggataatg aaacattcag
       61 ccagctttgt cataagccag aataatgtca cattcacacc tgacatatat ccatcactgt
      121 ttactaattt ctgttctgcc ctccctgctc cggctcacgt ctgaacgtac cgctgctgtt
      181 tcagatgtcg cagtaacacc ctgccctccg gcataaatgt tgttccgtaa cgaaccagtc
      241 cggcatcttt aagttctgca tcaatgaaat aatgcaactc accaggctga gtctgctccg
      301 gcatcgtcac gtcgacacgc ttaatacgtg gctcatactt cagcagaaca tctgaaagca
      361 cacacatcag ttgatgcgcc gtacccggca ttccttgcag aatgtttgcc atatccggca
      421 gaccataatc cggaagatgt ttaagacttc cggcccgggt attcagaata cgctgcatgt
      481 tgtccagaat agagagtata atctgctcat gttcacaaag aacattaatt tcaaatcctt
      541 ctgaaaaatt accatacagc ttctcataaa gcgataactc agccattaat caccatattc
      601 tatttatgat gctaatcaca agtgtctgca ctctgacata cagactaact aaatacactc
      661 agcaactaat ataactggag ctttacagaa tcatttaccg aaatatctcc atccatgcta
      721 acaataacat cctcagaaat agcctttact cttgcgcctc ccttaccgtt atatattaac
      781 gcataagagt atgcagctct gacttcaaag ccagcgcgtt cccgctgatt atagttaaat
      841 gtgacaatgt taccttctgt tttaatcaca t
//
LOCUS       DAZFFG010000351          866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFFG010000351 DAZFFG010000000
VERSION     DAZFFG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.354"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>866
                     /locus_tag="ACHJ7J_005181"
     rRNA            <1..>866
                     /locus_tag="ACHJ7J_005181"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          220 a          195 c          285 g          166 t
ORIGIN      
        1 gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata
       61 ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc
      121 cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt
      181 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
      241 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      301 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      361 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      421 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      481 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      541 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      601 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      661 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      721 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      781 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      841 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAZFFG010000352          862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFFG010000352 DAZFFG010000000
VERSION     DAZFFG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.355"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            113..784
                     /locus_tag="ACHJ7J_005182"
     CDS             113..784
                     /locus_tag="ACHJ7J_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5003243.1"
                     /translation="MYCDITDLVPKTIQYKYIKSYGPLNKYQLKRIEQTIDKALGEYP
                     RVMAIRVELHMPVRRPLCDMLLADDYARTDAAAISRFNASLNAKIKHYELKQKKAGKR
                     VRETSSRIVWAREFCPTNNKKHYHVLLLLNRDAFNSLGSFESDNGILLRLIREAWMSA
                     INLIYPKNKELVQIPDNPCYYISAKNGKQSEAYRELIFRSSYLAKEESKCFTDWERNF
                     GCSIK"
BASE COUNT          286 a          132 c          194 g          250 t
ORIGIN      
        1 taagtgataa taggttagtt ataacaggac ccatgctaat taaatatcat gaagtagtta
       61 ctttatgttg gtaatgtaaa tcgaaggatg taatcagtgg tgagggtaga ttatgtattg
      121 tgatataaca gacttggttc cgaaaactat tcagtataaa tatatcaaat cctatggacc
      181 cctaaataaa tatcagctaa aacggataga gcagacaatt gataaagcac tgggtgaata
      241 cccaagggtg atggcaataa gggtggagct tcatatgccc gtacgcaggc cgctctgcga
      301 tatgctcctt gccgatgatt atgccagaac agatgctgct gcaatctcac ggttcaatgc
      361 ttcacttaat gcgaagataa agcactatga gcttaaacag aaaaaggcag gtaagcgggt
      421 acgtgaaact tcatctcgta ttgtgtgggc cagggagttc tgtcctacga ataataaaaa
      481 acactatcac gttttgcttc tacttaaccg ggatgctttt aatagtcttg gatcgtttga
      541 gagtgataat ggaatactgc ttcgtcttat ccgtgaagca tggatgagcg ctattaattt
      601 gatttatcct aaaaataaag aactggtaca gatacctgac aatccatgct attacataag
      661 tgcaaaaaac ggtaagcaaa gtgaagctta cagagagctg atatttcgct caagttatct
      721 tgctaaagag gaaagtaaat gttttacaga ttgggagcga aattttggtt gtagtattaa
      781 gtgaagaggt acagtgggtt atgagacttt ttaagtaaag attttttaaa agaacaacag
      841 tatcgattat attttcgaag gg
//
LOCUS       DAZFFG010000353          851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFFG010000353 DAZFFG010000000
VERSION     DAZFFG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.356"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>851
                     /locus_tag="ACHJ7J_005183"
     CDS             <1..>851
                     /locus_tag="ACHJ7J_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5003244.1"
                     /translation="LYMLKRDFNSAISQARLTVTTPMISLYPYIISNVLYILPLSIIL
                     SFVLYFLWQHWMSRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQRE
                     DGKNISPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHP
                     DFVGDVLHIKNAISDKFNIVLEITERNLVEDTDLALQKINELRIHGCEFAVDDFGTGY
                     CSLGLLQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTPHQ
                     AEYLIHN"
BASE COUNT          261 a          148 c          170 g          272 t
ORIGIN      
        1 gttatatatg ttaaaaagag attttaattc tgcgatcagt caggctcgtc ttacagttac
       61 tacgccgatg atatcgttat atccctatat cattagcaat gtgctttata ttttgccgtt
      121 atctattatt ctttcttttg ttctctattt cttatggcag cattggatgt cacggaaaat
      181 gtcgctggca gaagaaatta aaaaaggaat gtcatccgga gaattttctg tgcattatca
      241 accagtgtgc gacacgacaa ccaaagcgtg tctgggggtt gaagcgttaa tgcgctggca
      301 acgcgaagat ggtaaaaata tatctccggt agtttttatc cgcgctgcag aagaggaaaa
      361 tttaataata ccgttaacta aacatctctt tgaactgatt attcaggatg tacaaagctg
      421 gaaagtgaaa aagccgtttc accttgggat aaatattgcc gcggaacatc ttgcacatcc
      481 tgactttgtt ggtgatgttt tacatattaa aaatgcaata tctgataaat ttaatattgt
      541 ccttgagatt accgaacgca atctggttga agacactgat cttgcattac agaaaataaa
      601 tgagttaagg atccatggct gcgaatttgc agttgatgat tttggcacgg gatattgttc
      661 ccttggcctt ctgcaaaagt tgtctgttga ttatcttaaa atagataaaa gctttataga
      721 cacactcact acagcggaag acgaaacgcc agttttagat attattatta agctgagtaa
      781 tcgactcaat ttgataacga ttgcagaagg tgtcagtact cctcaccagg cagaatatct
      841 tattcataat a
//
LOCUS       DAZFFG010000354          841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFFG010000354 DAZFFG010000000
VERSION     DAZFFG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.357"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..803
                     /locus_tag="ACHJ7J_005184"
     CDS             <1..803
                     /locus_tag="ACHJ7J_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO5003245.1"
                     /translation="YLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVL
                     SFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIP
                     EMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNIAFNMNNI
                     YLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNI
                     AREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          277 a          154 c          153 g          257 t
ORIGIN      
        1 attatttaaa gtcatttaat gaaacagcaa aacaatttaa tacctcaatt attgtatatt
       61 taattgaccc caaatacttt gctgatctgc cgacgtcaca gttttggtcg tacgcgacat
      121 acttcagggt attgtctttt gaatatctga gtgaaagtat ttccacactg ctgtatctgg
      181 atgccgatgt tgtttgtaaa ggaagcctga aacctctcac agaaattata tttaaagatg
      241 agtttgctgc ggtcattcct gacaatgata gtactcaggc ggcatgtgca aaacgcctca
      301 acattcccga aatgaatgga cgttatttca atgcaggcgt tatctatgtc aatcttaaaa
      361 aatggcatga agcaaatttg acaccgtatt tactcaaact tttacgaggg gaaactaaat
      421 atggctctct taaatattta gatcaggatg cgttgaatat cgcatttaat atgaataata
      481 tctaccttgc gaaggatttt gatactattt ataccctgaa aaacgaactt tatgatcgta
      541 gtcatcgaaa gtatcagcaa accattaccg ataaaacagt gttgattcac tatacaggga
      601 taactaaacc atggcatagc tgggctggat atccgtctgc atcatacttt aatatcgcgc
      661 gtgaacaatc tccctggaag aaatatcctc ttaaagaggc gcggactgtt gcagaaatgc
      721 agaaacaata taagcatctg tttgcccatg gtgagtatat aaaaggcata acttcattaa
      781 ttaagtacaa gcttaagaaa taaattactc ttatgattac aagtatacgt tatcgcggct
      841 t
//
LOCUS       DAZFFG010000355          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFFG010000355 DAZFFG010000000
VERSION     DAZFFG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.358"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..562
                     /locus_tag="ACHJ7J_005185"
     CDS             <1..562
                     /locus_tag="ACHJ7J_005185"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5003246.1"
                     /translation="YIQNRTVQSSVMNTHGSEWYLSQRMRVEHTELFNQLFSQFRISG
                     SNIIREDIIFFILDIFNSYQNTFPLLINCLYSYTSKVESIIYTNISYNWHVKDIACLL
                     HISTSLLKKKLREENTSFCRIKNQIRMRMAVDMLEKSNRSIDNIARECGYNDTSHFIC
                     IFKKYFSFPPGKYKNRISNSSELIHE"
BASE COUNT          314 a          156 c          137 g          227 t
ORIGIN      
        1 atatatacaa aacagaacag tacaaagctc tgttatgaat actcatggct cagagtggta
       61 tctctcacaa agaatgagag tggagcatac ggagcttttt aatcaacttt tttctcagtt
      121 ccgtatatcc ggaagcaaca taataaggga agatattatt ttctttattc ttgacatctt
      181 caacagttac cagaatacat ttcctttatt gattaattgc ttatacagct acacatcaaa
      241 agtggaatcc atcatttata caaacatatc atataactgg catgttaaag atattgcatg
      301 tttactgcat ataagtacaa gcctgttaaa aaagaaactc agagaggaga acacaagttt
      361 ttgcagaata aagaaccaga taagaatgag aatggcagtt gatatgctgg aaaaaagcaa
      421 cagaagtata gacaatattg ccagagaatg tggctataat gatacatcac attttatctg
      481 catcttcaaa aaatattttt catttccacc gggaaagtat aaaaacagaa taagtaattc
      541 ctcagagctt attcatgaat aaatatgaaa taaccacttc tgtaatacac atatacctca
      601 gatatgatag aataaaacag tcgcgtttct ggcatctttt caggtcgaag ggacagtccg
      661 gcaaatcata tgccagaaac gcgcatcaga aatgcagcaa caaagattac atccaattcg
      721 agggctacat acaacacttg ctgtccacag catgtctttc cgaaaaacgt ctacaaacat
      781 acagaaatcc acaataagat atctgaagcc tgacaaagtc tgcagactct gact
//
LOCUS       DAZFFG010000356          828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFFG010000356 DAZFFG010000000
VERSION     DAZFFG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.359"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..505)
                     /gene="yhaB"
                     /locus_tag="ACHJ7J_005186"
     CDS             complement(<1..505)
                     /gene="yhaB"
                     /locus_tag="ACHJ7J_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO5003247.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILF"
     gene            complement(761..>828)
                     /locus_tag="ACHJ7J_005187"
     CDS             complement(761..>828)
                     /locus_tag="ACHJ7J_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO5003248.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          245 a          155 c          160 g          268 t
ORIGIN      
        1 aaaagagaat ttgccaacaa tctgaagtaa tgctggtttt attaaagtgc acaaggggtg
       61 ttaagatggc ttcttttcgg gtgaattttg cacaatactt agaaatcgtc tccttaatac
      121 attccagctc ttttttattg acggctgagc aagtacattg ttcgccaaaa ggtgcaaaag
      181 tgcctacatc taatgtttgt atgatataat catcatgttc tcctgtagaa atatctatga
      241 tgaatggact catattttcc ggttggattc ggatcatgac agaaggaaca tcgccttgat
      301 agaaattcaa tgcagtaatt gcagataata ctctccgcag ttcattactt atactgtata
      361 ttttattaac gtcattggtg gagacaattt ctgcaaactt tctttttacc gtccaataat
      421 caatatttct attgtgattg ttaaccactg cgtgcattgg tgggattgaa gggtgaaaaa
      481 tatgtgcaat tgggaagcct ttcattgatg cctgccttgt catcatgaca aacaataata
      541 gatgcttatt ttcctcgcag ggttctttcg cactatcaaa aaacgctcat ttacccggcg
      601 ttacagtaaa cgaagcaaca ttctgtggcc tacgccgatc aaaagctctg tgaaaatttc
      661 ccttgttgtc tttgtctgac cttatcgcgg cgatcagcaa ttattcgttt tgtgatcctc
      721 aacacaaaat gctattcgtc gcctatgaag ggagaagaat ttatggaaaa cgattgcatt
      781 cacaaaatga cgtcctgtca gaagagaaaa agttgacgtt aatcaatt
//
LOCUS       DAZFFG010000357          818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFFG010000357 DAZFFG010000000
VERSION     DAZFFG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.360"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>818)
                     /gene="yjgL"
                     /locus_tag="ACHJ7J_005188"
     CDS             complement(<1..>818)
                     /gene="yjgL"
                     /locus_tag="ACHJ7J_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO5003249.1"
                     /translation="LNNSQELNNPQELNNSQESNDSQELNNSQDLNNSQVSCKGSVDS
                     TITDLLETTLNNALLAIRNEHLLLMPHVCNESISYLLSENGILEEIDKLYELNDHGID
                     NDKVGNNEINEIKVNLSHILIDSLDDAKVNLTPVIDSILETFSKSPYINDVRILDWCF
                     NKSMQYLGDSEKITHACSVMSHIDFDCNQSKIAEALFFNLNKEPYKNSPELQELIWEK
                     LVVYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFLKVFFMKQPDF"
BASE COUNT          268 a          153 c          111 g          286 t
ORIGIN      
        1 caaaatcagg ttgcttcata aaaaaaacct tcaaaaaaat atcatttacc aaaatgctga
       61 caggcacttc attaaatagc aacttaacat catcaaataa ccttagtatt aatcgcgatt
      121 tttcttgatt acttaaatta aaatcattga catatacaac caacttctcc caaatcaatt
      181 cctgtaactc ggggctgttt ttatagggtt ctttattgag gttgaaaaat aatgcctcag
      241 ctattttaga ttgattgcaa tcaaaatcaa tatgactcat cacggagcat gcatgcgtta
      301 tcttttcaga atcaccaaga tattgcatac ttttattaaa acaccaatcc agtattctta
      361 catcattaat atatggggat tttgaaaaag tctccagaat cgaatcgatg accggtgtaa
      421 ggttaacctt tgcatcatct aaggaatcaa taagaatatg agacaggtta actttgattt
      481 cattaatttc attgttacct actttgtcat tatcaattcc gtgatcattt aattcgtaga
      541 gcttatctat ttcttccagt ataccatttt cgctcagtaa gtatgaaatc gattcattac
      601 atacatgagg cattaatagc agatgttcgt tccttattgc taataatgca ttattcaatg
      661 ttgtttctaa taaatccgta atcgtagaat caactgaacc tttacaactc acctgcgaat
      721 tatttaagtc ctgcgagtta tttaactcct gcgaatcatt tgactcctga gaattattta
      781 gctcctgtgg attatttaac tcctgcgaat tatttaac
//
LOCUS       DAZFFG010000358          807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFFG010000358 DAZFFG010000000
VERSION     DAZFFG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.361"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(153..716)
                     /gene="ipbA"
                     /locus_tag="ACHJ7J_005189"
                     /gene_synonym="fimX"
     CDS             complement(153..716)
                     /gene="ipbA"
                     /locus_tag="ACHJ7J_005189"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO5003250.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          240 a          160 c          166 g          241 t
ORIGIN      
        1 ttggcggtta atcatttcaa atcatggggt tattttactt tagcgagaaa tatcaacatg
       61 ttatgtgttt tattgaattg aatgggctgt ttttttatgg aagaattgag gaaaacgtag
      121 gaatctgaca ttttgaatca gaaggtactg ttttaacgag gctttttttt ccacacccct
      181 ttaaatcgag cagcattact ggctgtgtat ctgacagtat gctggatatt tcgatgaccc
      241 agataatcct gaagtagtcg ggtatcgaca ccattgtcag ccagagcaaa accacaggca
      301 tgacgtaaca tatgaggatg tgcttttaca gctaatcctg cacgtcttcc agcctcacga
      361 atgatagaaa aaaaatgttg tctgctgata gggcggcgtt tccgggagag aaaaagccag
      421 tctccttcaa ctccgttttc aattaacttc ctctgcttaa gccacaactt gatcaaatta
      481 tattcatcag gaagaagagg atgtgttgtc gagaaaccat ttttaatccg ccgaatatta
      541 agttgttttc ctgatgcatc aatatcggat aaacgtaaat caagaagttc actggcccta
      601 aaaccgtgaa tgaaagccat cataattaga cagcgatttc tttcaggaaa ggacatgctc
      661 tgggctgcat ccatcagcct gtagacttca tcctgtgtga gatattttct cgtcatacgc
      721 caccgtgtac agttgtaaaa aagagaggca cacccagata agtatgcctg caatcagaac
      781 gttatcgatg taatctcatt atctcat
//
LOCUS       DAZFFG010000359          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFFG010000359 DAZFFG010000000
VERSION     DAZFFG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.362"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          248 a          155 c          153 g          239 t
ORIGIN      
        1 taagtgttct ttgctttaat gttaatgata ttcattggca ttatcgaaca gtgaatatct
       61 tgagcaggtt ttatatttat gcccaagata tatatttcta ataaatcatg atgccaatat
      121 catatcaaga tagtgaaact ggctccttga aatacagaca gtggatgtgt cttaatataa
      181 gagacaggct tggatatatg tctgacacta gttctaattt tgagatgact ggaactttgc
      241 tgggacagga gcttcgtaaa cgtaaactcc tcaggaaaag atcgccatta ttcagcagat
      301 aatgtaacct ggcatgacgg ttgtcccatg tggcgtgttt gcatggtatt cagcccagca
      361 atataggggc aaatccagac accatctcaa aaacggagga tgtatgccaa aattgtagca
      421 agactcagaa aagtctccag aggacgagtt aagtctgaaa tcttaagcaa agatatacga
      481 tatcggtcca tgcaaggtag aagtaagctt attattatca gaagcacgga ataccaactt
      541 ttgacatttt atgtatccaa cgatgactgc aataccttta ttgccccctc ctgttctgtc
      601 tcatcccctt tctgacacag gccatgcccg gctgtcagtc tgactgaatt tgatatcgct
      661 atggataaat catctgagaa atataattat ttatacagta agacccatat cccgccacgg
      721 ccctccaggt ctgcagacat atatgtcttc aatctggttt tccgtatttt tatgaaaaat
      781 tataatatga aacaa
//
LOCUS       DAZFFG010000360          792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFFG010000360 DAZFFG010000000
VERSION     DAZFFG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.363"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..192
                     /locus_tag="ACHJ7J_005190"
     CDS             <1..192
                     /locus_tag="ACHJ7J_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003251.1"
                     /translation="DIKKIDEKIEEIDNAIRDLSSETERFKIKYQQENTLNDVVQNIR
                     TWLKENQNMVKAIKKIDTE"
BASE COUNT          285 a          132 c          144 g          231 t
ORIGIN      
        1 gatataaaga aaatagatga aaaaattgaa gaaatagata atgcaatccg ggatctttcc
       61 tctgaaacag agagattcaa gataaagtat caacaagaga atacacttaa tgatgtagtt
      121 caaaatatca ggacttggct aaaagaaaat caaaatatgg tgaaagctat taagaaaatt
      181 gatactgaat aacatggcga ataaataaac ggatcaagtc gttcctatga caatgaggtt
      241 atacgaaaag acgcttactc atcagaaatc tggtatgctt taattaaacc agtgtcgctg
      301 atgtgtaagc gttgacgttg ggagacgatt aaatggttat accactccta cgtcttttcc
      361 atacggcgta taagagtgca acatgttgag ttttccgagt gaatggtgca ttttactaac
      421 cgagccattt gaatcaacca acgcaataaa aaagactgga ataataagcc tgtatttatt
      481 atttatcgca atcgcacttt acggttttat ccaactgaaa agagactgct tccagcggtt
      541 gtggattatc cctgggcgat agtcatagtc tggtgtcagg attcacaaat gacctgtttc
      601 cttacatgga tataaacaca acatagagtg cggtagttaa tctgaataat ctaatttcgg
      661 ctattcagca caaaacaaca ttattgattc tttataagag agaatctctc catcattttt
      721 atataaatca ttattaagaa aatccagaag ctcttgcctt acactataaa tattgacttt
      781 ttggtcaacc aa
//
LOCUS       DAZFFG010000361          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFFG010000361 DAZFFG010000000
VERSION     DAZFFG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.364"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            1..701
                     /locus_tag="ACHJ7J_005191"
                     /pseudo
     CDS             1..701
                     /locus_tag="ACHJ7J_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          305 a          143 c          120 g          212 t
ORIGIN      
        1 acccgacaag gcaatcataa ctaaaattag cgtagaagag ggaataccag taagattcag
       61 tgtccaaacc atgttttcag atactaattt tattgctgaa caacgagctg acttaccgac
      121 taatataaaa gatattcaaa gcctgtatca aaaaatgacg aagttatata ttgagcacag
      181 tgaaaataaa aacaggatga aagtctttgc cggaaccaac tttattgatt tcaatatgac
      241 aggacagaac ctgtccgggt tcgttctaac cttgtcaagg ttttatttcg aagatctact
      301 taatataaat ttcactgacg caaatttagg tgacactatt ttttcacata aagaacaccc
      361 taccccaaaa ttatataaag acgaacaata tcttgacaaa caaatcgaag gtttattttc
      421 aacattatta accattaatg acaacttttt acgagcaaaa gcggaaattg cttcgacaat
      481 aattaaattt ttagaggcca ggataaccaa tctttcatac aacgacatat taaaatatca
      541 gcaagaattc caaaaacaat gttataagca agttaaagcc tttacgacac tctcccgata
      601 caataaaatt caaacatggg cagaaatgtc tgaatatcaa ttcgaagtat ttcaatacga
      661 gacgcttaac ccaaaaaaaa tgtcacgcac gccttattta aagaggccac ttcctgatga
      721 aaaagatata aattatgggg tggaaataga aataccgtct ggtaaaagaa ttcgcttatc
//
LOCUS       DAZFFG010000362          779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFFG010000362 DAZFFG010000000
VERSION     DAZFFG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.365"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(121..684)
                     /gene="fimA"
                     /locus_tag="ACHJ7J_005192"
     CDS             complement(121..684)
                     /gene="fimA"
                     /locus_tag="ACHJ7J_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5003252.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          236 a          186 c          153 g          204 t
ORIGIN      
        1 aatacaaacc actcctcatt aataatgaga ttcacattgg tgaattgctc gggtgtgcgt
       61 agccatgcca cacacaccct gtttcaattt cagatgacgt atagccacct taagattgaa
      121 ttatagatat tgaactttga aggtcgcatc tgcgtttgca gtaccagcta cggactgccc
      181 gagtgcaata taacgagcct ggaacggcag tatattcgtg ccatcaatca agtcagtttt
      241 agcgctaaaa gtggcaccat caagtattaa tacctcaccc gtacggtcca aaatttggat
      301 accgacattt tgggcagagc ctgccgctga actctgcagc gctaacgtat cgtcattact
      361 ggtgacagta gtacccagga atgcaacagc tgcattactg gaaacattgg tatcacaatc
      421 attcagcttg atattgaagc caacggcgga gctgaggtca cccgctttag ccaggcgaga
      481 agaacgaacc tgacccagct caaccgtttg gttcattgat tcggaatcaa tcgcacaagc
      541 ggcatttacc acttcacctt taaaatgaat aacaccgcca ttaacagtgg tcgttactga
      601 ttcatcaccc tcggctgcgc ttaccgcagc agacgacatc gccaacacag aaacgacaat
      661 cataccaaca tgtttgagtt tcataattct ccaaaattta tatttaagta gctgaataaa
      721 gagagttaac aattaagtca ttctgttatt gcttataaaa caagaatcat tcttgattg
//
LOCUS       DAZFFG010000363          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFFG010000363 DAZFFG010000000
VERSION     DAZFFG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.366"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..573)
                     /gene="fimE"
                     /locus_tag="ACHJ7J_005193"
     CDS             complement(<1..573)
                     /gene="fimE"
                     /locus_tag="ACHJ7J_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO5003253.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINE"
BASE COUNT          242 a          184 c          146 g          197 t
ORIGIN      
        1 ttcgtttatg agattatttc tttcccataa tccggcaaaa cgagcagcat tactggcggt
       61 ataacgtaca gtatggcgaa tatttcgatg cccgagataa tcctgaatta aacgagtatc
      121 tgcaccacgc tccgccagtt cataaccgca agcatgcctt aacatatgag gatgagtctg
      181 cgttacggtt ccagcttcaa taccggcatc acgaataatg cgataggcct gctggcgaga
      241 aagccgactc ccgcggcgag aaataaatat agcgtcggtc cggtcagcgc ctttccagtt
      301 agcacgttcc agggtccagc gttccacagc ttcacgctca tcaaaacgta acgggtgaac
      361 ggtagaaaat ccgttcttca gtcggcgaat atttattcga ccttcattaa ggtcaagatc
      421 ctgataatgc agatcaagca gttcactaat acgcatccca tgccgatatg ccaacagaat
      481 aagacaataa tctctggctc ccgttgcccc gtaacaaacc gcctgcatca tggcctgaac
      541 ttctttaccg gtaagataac gacgtttact cacaatagta gtactcctaa ctgagatata
      601 tttgaatgaa tacctatagg aaacctcaat cggtcaaaat tagcccaata gataaaagat
      661 aaccacacac gaagtgatgt ggctatcagt caattacatt aaatatcata caaaaataaa
      721 atatcactga tgtgacaaca tctattttca ttgatttgat tcaatataa
//
LOCUS       DAZFFG010000364          745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFFG010000364 DAZFFG010000000
VERSION     DAZFFG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.367"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          265 a          130 c          111 g          239 t
ORIGIN      
        1 ggcaagaaac tcaaaaaaca acgtcaacgt aacgtcaact ctgaaagtgc tctttcggtc
       61 gctttttggg ttgaatgcac gtagaaatcc ccaaccatga tatttgagcg cataatcacg
      121 attatgaaag gttttttctt taggttaaac atatgtttat caaacataca atattatgac
      181 aacatcacaa ttatttaaat ccatgtctta tatttgacat catcaaaaaa gtaataaata
      241 aaaattacgc tatcaaaaat actaatgtga attttctacg actggacccg acaacgccta
      301 agttaggcat gcgattaaaa atcacaatct acattaagct atgagttcaa ctgatagatg
      361 taaataatgc ctagttaact gtgtcgttta agaaagatag tagagtgaaa tgaaagtata
      421 tcgctatcat atgcgttgag aaaaagctct aaaacctaaa ttcttaccat gcagccgatt
      481 actttataaa ttttagtttt acgcaagatt ttgataagtg gatgacatca ataatattca
      541 tttttattga tgtcatctac caggagtgtg ctttcaataa aacctgagtc gtttaagttt
      601 taaatctaag atcgtcattt cgaccacatc aatagaatac tttggaataa ttttcctaag
      661 attgaaaata acttccatca attcctgcat agcagccctt tcaatcttca tctcactgag
      721 gatagttttt attctgtaca acaga
//
LOCUS       DAZFFG010000365          729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFFG010000365 DAZFFG010000000
VERSION     DAZFFG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.368"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            31..>729
                     /locus_tag="ACHJ7J_005194"
     CDS             31..>729
                     /locus_tag="ACHJ7J_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5003254.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYH"
BASE COUNT          242 a          127 c          160 g          200 t
ORIGIN      
        1 tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa tattgttgct
       61 aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg tgtagaaatc
      121 tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt aaaattcgtt
      181 attgaacgtg caaaacaggc acaagttcca aagcacgtta ttgataaagc aattgataaa
      241 gccaaaggcg gcggagatga aacattcgtg cagggccgtt atgaaggctt tggccctaat
      301 ggctcaatga ttatcgccga gacattgact tcaaatgtta accgtacgat tgctaacgtt
      361 cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt cagctatatg
      421 tttgacaata cgggtgtgat tgtatttaaa ggatcagacc ctgaccatat ttttgaaatt
      481 ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa cattgttatt
      541 tatactgaac ctactgacct tcataaagga atcgcggctc taaaagcagc tggaatcact
      601 gagttctcaa caacagaatt agaaatgatt gctcaatcag aagttgagct ttccccagaa
      661 gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga tgtacaaaaa
      721 gtttatcat
//
LOCUS       DAZFFG010000366          729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFFG010000366 DAZFFG010000000
VERSION     DAZFFG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.369"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>729)
                     /locus_tag="ACHJ7J_005195"
     CDS             complement(<1..>729)
                     /locus_tag="ACHJ7J_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003255.1"
                     /translation="GLVQAATLLLDVVNTVNGGRVLATDSADVKGTTLNNTGTLQGAD
                     LLVNYHTFSNSGTLLGTSGLGVKGSSLLQNGTGRLYSAGNLLLDAQDFSGQGQVVATG
                     DVTLKLIAALTNHGTLAAGKTLSVTSQNAVTNGGVMQGDAMMLSAGEAFTNNGTLTAG
                     KGNSVFSAQRLFLNAPGSLQAGGDVSLNSRSDITISGFTGTAGSLTMNVAGTLLNSAL
                     IYAGNNLKLFTDRLHNQHGDILAG"
BASE COUNT          153 a          214 c          196 g          166 t
ORIGIN      
        1 ttgccggcca ggatatcacc atgctggtta tgcagacggt ctgtaaacag cttcaggtta
       61 ttccccgcat aaatcagcgc actgttcagc agggtaccgg ccacattcat tgtcagactg
      121 cctgccgtgc cggtaaaacc actgatggtg atatcactcc ggctgttcag actcacatcg
      181 ccaccggcct gaagtgaacc cggtgcgtta aggaaaagac gctgtgcgct gaaaacactg
      241 ttgcctttac cggcagtcag tgttccattg ttggtgaatg cctctcctgc actgagcatc
      301 atggcatcac cctgcatcac accgccgttg gtgacggcgt tctgcgacgt gacggaaagg
      361 gttttccctg cggccagggt accatgattc gtgagggcag caatcagttt cagtgtgaca
      421 tcaccggtgg ccaccacctg gccctgacca ctgaagtcct gagcgtcaag cagcaggttg
      481 cctgcactgt acaggcgccc tgtaccattt tgcagcagtg aactgccctt aacaccaagc
      541 ccggaggttc ccagcagggt accgctgttg ctgaatgtgt ggtaattcac cagcaggtcc
      601 gcaccctgaa gcgtaccggt attattcagc gtggttcctt taacgtcggc actgtcggtg
      661 gcaagtacgc gtccgccgtt gacagtattc accacatcca gcagcagggt ggcagcctgt
      721 accagtccg
//
LOCUS       DAZFFG010000367          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFFG010000367 DAZFFG010000000
VERSION     DAZFFG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.370"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     repeat_region   117..572
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=117..145
                     /rpt_unit_seq="gagttccccgcgctagcggggataaaccg"
BASE COUNT          189 a          173 c          180 g          170 t
ORIGIN      
        1 aatcaatggg ttaagtgctc tttaacataa tggatgtgtt gtttgtgtga tactgtaaag
       61 ttggtagatt gtgactggct taaaaaatca ttaattaata ataggttatg tttagagagt
      121 tccccgcgct agcggggata aaccgagaag cttatcaacg ttgccaatac gctggccgag
      181 ttccccgcgc tagcggggat aaaccgtttc caggcgttgg cggtcggcgt cggccaccga
      241 gttccccgcg ctagcgggga taaaccgacc gaaaagttaa agtagtgccc gtagttggtg
      301 agttccccgc gctagcgggg ataaaccgct ggaccgtcgg ataaaagcga caacgcagga
      361 gagttccccg cgctagcggg gataaaccgg ccggtcgtgt cgctgatagc ctcaacgcgg
      421 cgagttcccc gcgctagcgg ggataaaccg gtattttgtc acgaccagca cgaggccgaa
      481 tagagttccc cgcgccagcg gggataaacc gccgcaaaag gtattatttt tatccgtcag
      541 tacgagttcc ccgcgccagc ggggataaac caccatataa cccgttatct ctttctcaag
      601 tttttatatt agctgtactt gtaataagca acatatccac gtaacacctc atgttcaaaa
      661 tagttcccca tgccagataa gttcacaatt atcgatacaa aaatcaaatt ta
//
LOCUS       DAZFFG010000368          696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFFG010000368 DAZFFG010000000
VERSION     DAZFFG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.371"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..203
                     /locus_tag="ACHJ7J_005196"
     CDS             <1..203
                     /locus_tag="ACHJ7J_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO5003256.1"
                     /translation="MALELSYVYIKYVYGKEKAEFQKPYSITDDNNCWKIEGKQPKTL
                     GGNFTILIAKKDGQVLNVIHTK"
     gene            complement(416..>696)
                     /locus_tag="ACHJ7J_005197"
     CDS             complement(416..>696)
                     /locus_tag="ACHJ7J_005197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003257.1"
                     /translation="LLPVSRYGGAGSHKGKKFPAVRRPIVHGGNAPASIGYRSDNRVT
                     CRHIAYLMFILFHKPSVHIVIVKIQYIFADAVILLHVGSAEYCSRYGQ"
BASE COUNT          235 a          130 c          141 g          190 t
ORIGIN      
        1 aaatggcttt ggaattatca tatgtttaca ttaaatatgt atacgggaaa gaaaaagcag
       61 aatttcagaa accatatagt attacagatg acaataattg ttggaaaatc gagggaaaac
      121 aaccgaagac tttaggagga aattttacaa tactaatagc gaaaaaagat gggcaagtgt
      181 tgaatgtaat acacacaaaa taatccactg tacagtcgat tcaaatattt taccaaggtg
      241 tgtttcgtaa caattttgtg tacaatatga ctctacatat cgacgaaaaa accacttgat
      301 ttgacaggaa cacattcaaa aaaatttctt ttttcatttc aggagcgatt ttcatctgtt
      361 acttgaagta tctgtgagat taagtgactc cccctaacgt cgtactaaat agtaattact
      421 ggccataccg ggagcagtat tcggcgctcc ccacatgcag cagaataacc gcatcagcga
      481 agatgtattg tatttttact atcacaatgt gtacggacgg tttatggaaa agaatgaaca
      541 ttagatacgc gatgtggcgg caggttacac ggttatcact acgatagcca atactggctg
      601 gtgcattacc gccgtgaaca ataggacgcc gtactgctgg aaatttctta cctttatgac
      661 tccccgcgcc gccgtatcgg gaaacgggaa gcaatc
//
LOCUS       DAZFFG010000369          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFFG010000369 DAZFFG010000000
VERSION     DAZFFG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.372"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..680)
                     /locus_tag="ACHJ7J_005198"
     CDS             complement(<1..680)
                     /locus_tag="ACHJ7J_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003258.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIEMVTLLNALQPGGKCVITGDFQKELAYLQNVILYNDSSLRMDFFGYNARIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVVFSCGTGGIVDLSSLDLRN
                     VDLELYDFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPV
                     LTSISDMYS"
BASE COUNT          220 a          114 c          117 g          238 t
ORIGIN      
        1 gaatacatat cgctaatgct tgttagaaca ggcgttattt ttagtaaata atcccaggag
       61 attgtggtgt tttgttttga actaacgaaa tttacctgaa acatgttggc attagtaaaa
      121 tctgtgtcat ccaatttaaa aggatttaaa attgcattgg ccatatgttt atctgtaaaa
      181 tcataaagtt ctaaatcgac attccgtaag tccagagagg atagatcaac tatacctcca
      241 gttccacaac taaaaactac gtttaacctc ctgatttcat tgtaaatatc tttcaatgga
      301 caattaacat tgatattgcc tgtggatatt tcctggtttt ttaacggttc attaattgta
      361 agttcgcagg tattgtctga tcgttgaata attcgggcgt tataaccaaa aaaatccata
      421 cgaagagacg aatcattata aagaattaca ttttgtaagt acgctaattc tttttgaaaa
      481 tcaccagtga taacacattt cccacctggt tgtaacgcgt taagtaaagt gaccatttca
      541 atagaggtct gttttagttt ttctatacct ggtgctagtg cttcgaggtt attaactatg
      601 gggctttcag gaagatcatt catttttcga cggactaaat cactactaat atcattacca
      661 attgatgaaa cagggaacat tattttatc
//
LOCUS       DAZFFG010000370          677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFFG010000370 DAZFFG010000000
VERSION     DAZFFG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.373"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..603)
                     /locus_tag="ACHJ7J_005199"
     CDS             complement(<1..603)
                     /locus_tag="ACHJ7J_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5003259.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAE"
BASE COUNT          214 a          134 c          149 g          180 t
ORIGIN      
        1 ttccgccaaa tatcgcgttg cagaaagtcg gcaaataatt aacactttgt ctcctttagt
       61 gaaaattttt ccttcttcag gctccagtag taaataatgt atttggccaa attgatcggt
      121 gagttttcct tcgcagggat tacctgaagt ggcttgatga ccggtaatta atgccatgct
      181 accaatatat tcttcttctg taatagctga actatggtcg cgtggaatcc agggcgcaac
      241 tattttaccg gtataatgca ccgcaataat cgtaaacagt aaactgacag gaaccacgaa
      301 caggttcgac agtggtgatt gccagaccat gatgcaggca tgctgcaaca ggatgccaat
      361 aagaccaaaa aaaccagcca gcaagcagag aacgacgaga gctggcaatc ttccgatatt
      421 gagataatga agtgcctggc taatatgacc tgtggttatg gaatcataat ggtcaagatg
      481 tgcatcaaga gcaccggaga gcatgtgacc gcagataagc gcaaaaattt ctaaaaggcc
      541 gatcaacagt acaaaggaaa tagcgaaaag ataaggggta ttatagtcgg cgaataaaat
      601 catatgtcac tccaatgtcc atttagtgac gcaatattta ctttgggata gtttccttgc
      661 ttacatttat ctttgtt
//
LOCUS       DAZFFG010000371          666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFFG010000371 DAZFFG010000000
VERSION     DAZFFG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.374"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>666
                     /locus_tag="ACHJ7J_005200"
     CDS             <1..>666
                     /locus_tag="ACHJ7J_005200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003260.1"
                     /translation="RCKFQIDGSSPSKKYILPFSARDITAGADINTGTVLSTVRYNKP
                     IQIQCEPTSGAPSDEEITIKYYLQVLTTPYPSSGMIAANGLPVYNTPISGIGFALDSA
                     NPVATPTYPALMDVQTIAANISDIRYIFGTNALLMRLYKTGAPGSGAINGEDLPTLKT
                     FITTESNYDPVPKQEDMAFISFSGVMNVVSYTCRTPDVVVSLGEHETNIFTTPGTSTQ
                     WVD"
BASE COUNT          211 a          150 c          127 g          178 t
ORIGIN      
        1 caagatgtaa gttccagata gatggttcaa gtccgtcaaa aaaatacata ttaccattct
       61 cagcccgaga tatcacagcc ggagccgaca tcaataccgg tacggttctc tcaaccgtca
      121 gatataataa acctattcaa atacagtgtg agccaacatc cggcgcacca tctgatgaag
      181 aaataacaat taagtactat cttcaggtgt tgactacccc ttacccgtca tcaggaatga
      241 ttgctgccaa tggattacca gtatataaca caccgatttc aggaattgga tttgctctcg
      301 actcagcaaa tcctgtagct acaccaacat atcctgcatt aatggacgta cagacaattg
      361 cggctaacat atctgatatc aggtatatct ttggtaccaa cgccctatta atgcggttat
      421 ataaaacggg ggcaccaggg agtggagcta taaatgggga agatttaccc actttaaaaa
      481 catttattac aaccgaatcg aattatgatc cagtccctaa gcaagaggat atggccttca
      541 tttcattttc aggtgttatg aacgtggtct cctatacttg cagaacacca gatgttgtag
      601 tttcattagg cgagcatgag accaatatat ttacaacccc aggaacttcg actcaatggg
      661 ttgatt
//
LOCUS       DAZFFG010000372          659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFFG010000372 DAZFFG010000000
VERSION     DAZFFG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.375"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>659
                     /locus_tag="ACHJ7J_005201"
     CDS             <1..>659
                     /locus_tag="ACHJ7J_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003261.1"
                     /translation="ATTLDGYGLLQGAGALALAGDTLSQGRNGRWLTAGDLTLRGKTL
                     HTAGTTQGQNLTVQADNWANSGSVLATGNLTASATGQLTSTGDIMSQGDTTLNAATTD
                     NRGSLLSAGTLSLDGNSLDNSGTVQGNHVTIRQNSVTNSGTLTGIAALTLAARMVSPQ
                     PALMNNGGSLLTSGDLTITAGSITSSGHWQGKRVLITADSLANSGAIQAADSLTARLT
                     G"
BASE COUNT          144 a          203 c          200 g          112 t
ORIGIN      
        1 gcgaccacgc tggatggcta cggcctgttg cagggcgccg gtgcactggc gcttgcaggc
       61 gacaccctct cacagggccg taacggacgc tggctgacgg cgggcgacct caccctccgg
      121 ggtaaaacac tgcataccgc agggaccacg cagggacaga atctcactgt gcaggcggac
      181 aactgggcga acagtggttc tgtgctggca accggtaacc ttactgcttc ggcaaccggt
      241 cagttgacca gtaccggcga tatcatgagc cagggtgaca ccacgctgaa cgcagccacc
      301 acggacaacc ggggcagtct gctttcggcc ggcacgctct ctcttgatgg aaattcactg
      361 gataacagcg gcactgtcca gggtaaccat gtcacgattc gtcagaacag tgtcaccaac
      421 agtggcacgc tcaccgggat agccgcgctg acgcttgccg cccgtatggt atcccctcaa
      481 cctgcgctga tgaataacgg aggttcattg ctgaccagcg gcgatctgac aatcaccgca
      541 ggcagtatta ccagttccgg acactggcag ggcaaacggg tgctgatcac cgcagacagt
      601 ctggcaaaca gcggggcgat ccaggcggct gacagcctga ctgcacgtct gacgggtga
//
LOCUS       DAZFFG010000373          655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFFG010000373 DAZFFG010000000
VERSION     DAZFFG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.376"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..393)
                     /locus_tag="ACHJ7J_005202"
     CDS             complement(<1..393)
                     /locus_tag="ACHJ7J_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003262.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQTQEILNQINNIRYEKNKSAECR"
BASE COUNT          176 a          109 c          130 g          240 t
ORIGIN      
        1 tcgacattca gcacttttgt tcttttcgta tctaatgtta ttaatttgat taagtatttc
       61 ctgtgtctgc cgcgtaagtg gtgtcgaact tatcgattca atgttagtaa taagacttac
      121 aagttgagcc acaacttttg agtagtctgc atctgtatat ttggtgttcg tgcggcacgc
      181 ataaatttgt gggaggtgtt gccgcgctaa atcatattgt tcagggttgt atccttctcc
      241 agagaatgtg gaattaatgg gttgttgttc ttcccgatta tcacaaggcc acatcggtgt
      301 gtctggtgtt ttaacattgt cggtatctgt tgtgcggtta taaatatcgc cgtgatatgt
      361 atttatcata cctggggtaa agtcaattga cataacattc cttattcatc atctgtttta
      421 tgctaagatt tttaaattgg ataattagag caggaatgga ttttcttcta cgcacaataa
      481 atatcatgcg tcatatcttc ctgatataat tacctttgac ttccctttta tctccacccg
      541 gtaatatttg tgtataaaaa agggcattga tagtatcgta aaggaaaacg ttaacaacta
      601 tcaaaaagcg ctcattttaa aaagttatga tgttctccga tagtgtgtga aatga
//
LOCUS       DAZFFG010000374          651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFFG010000374 DAZFFG010000000
VERSION     DAZFFG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.377"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            187..>651
                     /locus_tag="ACHJ7J_005203"
     CDS             187..>651
                     /locus_tag="ACHJ7J_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFO5003263.1"
                     /translation="MVDNVTGGSVWTQSPSFMPELDGEKNKTQLIIDDIVAYLKNPSV
                     YSLEKEGPLNHFFNNCSEIEPWVLIGGGLIIIVSTSEQDPESLIVTVGDIEGKCATQI
                     PVPPALTTSLKDIFIRPHTNAKYESFTKEQIKANIKSNVINYNCKLHFNEKPE"
BASE COUNT          221 a          106 c          126 g          198 t
ORIGIN      
        1 tccattacaa ggagcggttt cccacgttga cgtcccggtt tacccacctt attaccaggt
       61 tgatgacctg atggggtaat gacgtagaaa gacaaaggaa tatacaaatg agcgaaattt
      121 gatagttgat ttttaaatag taatcttatt attactacaa ctttcatatg aatgaggaga
      181 aagaaaatgg ttgataacgt aactggtggc agtgtatgga cacaatctcc ttctttcatg
      241 cctgagttag atggagaaaa aaataaaact caattgatca ttgatgatat agtggcgtat
      301 cttaaaaatc cttcagttta ttcgcttgaa aaagaaggtc cgttaaatca tttttttaat
      361 aattgtagcg agattgaacc atgggtttta atcggtggtg gacttattat tattgtctcc
      421 acatctgagc aagaccctga aagtctgatt gtaactgtgg gggatattga aggaaagtgt
      481 gctacacaga ttcccgtacc acctgcgctt acaacatccc tcaaagatat ttttattcgc
      541 cctcatacga acgcaaagta tgagagtttt acaaaagagc agataaaagc caacattaaa
      601 tctaatgtta taaattataa ttgtaaattg cattttaatg aaaaaccaga a
//
LOCUS       DAZFFG010000375          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFFG010000375 DAZFFG010000000
VERSION     DAZFFG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.378"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>650)
                     /locus_tag="ACHJ7J_005204"
     CDS             complement(<1..>650)
                     /locus_tag="ACHJ7J_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO5003264.1"
                     /translation="GKVPLKACYSSPEDIQKHIPFELEQQFDNLQKNPPPGTCVVACD
                     KFGEALSVFFHRMEKEKLTHMAAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPNAT
                     NTAVRYKANNCDSFGSLRSFMNIKQAQKQWVITEICSDCVGITPYLPREQAHLLSGIN
                     NELQPPLSPSALHLLIQMGMHENIVLFFDKFKNSQEMTASKILAAKSITGTYGLYV"
BASE COUNT          184 a          114 c          128 g          224 t
ORIGIN      
        1 atacatataa tccatatgtt cctgtaattg atttcgcagc aagaatcttt gatgctgtca
       61 tttcttggga gtttttaaat ttatcaaaaa aaagaacaat gttttcatgc atccccattt
      121 gtattaatag atgtaatgct gatggtgata atggtggttg caactcatta tttataccac
      181 ttaataaatg ggcttgttct cgagggagat aaggagttat tcctacacag tcggagcaaa
      241 tttctgtgat tacccattgt ttttgtgcct gtttaatatt cataaaggat cgcaatgatc
      301 caaaagaatc acagttgttt gctttatagc gtactgcagt attggttgca ttaggatcat
      361 aaaaggatac aacatattct gtttcaccag caggcgtttt tttgattctc aagcggacgg
      421 ccatagcgtg agtttgagat tgaacgattg ccgccatatg cgttaacttc tctttttcca
      481 ttctgtgaaa aaagactgat aatgcctccc caaacttatc acaggcaaca acgcatgtac
      541 cgggtggtgg atttttttgt agattgtcaa attgctgctc aagctcgaat ggaatatgtt
      601 tttgtatatc ttccggagat gaatagcaag cctttaatgg aactttccct
//
LOCUS       DAZFFG010000376          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFFG010000376 DAZFFG010000000
VERSION     DAZFFG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.379"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            73..639
                     /locus_tag="ACHJ7J_005205"
     CDS             73..639
                     /locus_tag="ACHJ7J_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003265.1"
                     /translation="MKKITKQTKNTIFIVAALVGICSYIFPRSPDTQKTDDIVGVWTS
                     DYSYGTQSVLAGVNVRTSYFSNGKYNVNGRMSFKIDNANGKTALVEYYVSGAGEWVLH
                     DKELIATLSSLKSIPDRIVSDGKDFDIQDLEILENIMRKKFPTIEERMAPGTSQSYII
                     KNDSHNQKLLEVINPFGNNFDITITREK"
BASE COUNT          230 a          107 c          127 g          186 t
ORIGIN      
        1 atttattttc agaaatagat tgaaaatgat aaaatgcacc tatctctttc actcattcta
       61 aatggaattt ttatgaaaaa aataaccaag caaacaaaga atacaatatt tattgtggca
      121 gctcttgttg gaatctgttc ctacattttt cccagaagcc ctgatactca aaagacagat
      181 gatatcgttg gggtgtggac atctgattat tcatatggca cacagagtgt tcttgctggt
      241 gtcaatgtga gaacttctta tttttctaat gggaaatata atgtaaatgg tcgaatgtct
      301 tttaagatag ataatgcaaa tggaaaaaca gcccttgttg aatattatgt tagtggtgct
      361 ggagagtggg tactccacga taaagaactg atagccacac ttagcagcct gaaatcaatt
      421 ccagaccgga ttgtctctga tggaaaagac ttcgacattc aggatcttga aatattagaa
      481 aatatcatgc gcaaaaaatt tccaactatc gaggaaagaa tggctccagg aacaagccag
      541 tcttacatca tcaaaaatga cagtcataat caaaagctac tggaggtaat aaatccgttt
      601 gggaacaact ttgacataac cataacaaga gagaaatagg gctgggattg
//
LOCUS       DAZFFG010000377          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFFG010000377 DAZFFG010000000
VERSION     DAZFFG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.380"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..466)
                     /locus_tag="ACHJ7J_005206"
     CDS             complement(<1..466)
                     /locus_tag="ACHJ7J_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFO5003266.1"
                     /translation="MKMFSKNKITIAAISALLISGTAMAQTSSGSVSATGSFNAATCS
                     VTLPNATASFGTVTNQEVNAAKAYQKIKSVTADQGISVTDCTTPASLTVATTNFQSNG
                     YVYPALNEATGNQQKDFALWMKVGDKDVSSYNAPIELANGTYPIELQMIKISN"
BASE COUNT          197 a          124 c          121 g          205 t
ORIGIN      
        1 tatttgatat tttaatcatt tgcaactcga ttggatatgt accattagca agttcgattg
       61 gtgcattata agaagataca tctttatcac cgactttcat ccaaagcgca aaatcctttt
      121 gttgattacc ggtagcctcg tttagagcag gataaacata gccattagac tggaaattag
      181 tagttgctac cgttagtgat gcaggagtgg tacaatcggt aacactaatc ccctgatctg
      241 cggttacact ttttattttc tggtacgctt ttgcagcatt tacctcttga ttcgttactg
      301 tgccaaaaga tgctgttgca ttaggcaggg taacactgca agtcgcggca ttaaaagaac
      361 cagtagcgga gacagaacca ctgcttgtct gggccattgc ggtaccagaa ataagcagag
      421 ccgaaatagc tgcgatggtg attttattct tagaaaacat tttcatgttc aacctcaatc
      481 ttgattttct taaatttttc ctatgtgtat agtgatgggc aaagagatgt gagaataaaa
      541 ctttcaaaat ttagcaacac atcccaatgc ctcttttcta ttcttcaaaa aaaaagatta
      601 attcaagcca taaaaatgca accattcgat acttatttgt acatgac
//
LOCUS       DAZFFG010000378          637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFFG010000378 DAZFFG010000000
VERSION     DAZFFG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.381"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..205)
                     /locus_tag="ACHJ7J_005207"
     CDS             complement(<1..205)
                     /locus_tag="ACHJ7J_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003267.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKKATLTIWQGVIP"
     gene            complement(224..508)
                     /locus_tag="ACHJ7J_005208"
     CDS             complement(224..508)
                     /locus_tag="ACHJ7J_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HFO5003268.1"
                     /translation="MPLAAKLTDKGTQHDGYYETVITAGSSTVFIDGLPAARQGDPLT
                     PHAKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDCGGVVIGGGTVNIG"
BASE COUNT          160 a          168 c          158 g          151 t
ORIGIN      
        1 gcgggatgac gccctgccag atggtcagcg tcgccttctt ctccagcagc atctccgccg
       61 tctgcataaa agagtcgctg gcaacatcca cactcatcac aaacggatat gactggttct
      121 ggattaacct gaaattcacc actgcaaagg tgtccggctc ctggccgtca acctccagcg
      181 taaagcgcag accttttaag gacataaata cctctcagac atatcagcct atatttaccg
      241 taccgccacc aatgaccaca ccaccacagt cgatggcatc accggttctg gcggcgggca
      301 ggccgtcaat aaagacggtt gacgagcctc tggcgatttt acggggatga gggggatgct
      361 tcggtttagc atgcggcgtc agcggatctc cctggcgggc tgcggggaga ccatcgataa
      421 aaacggtgga tgacccggcg gtgatgacgg tctcgtaata accgtcgtgc tgggtgcctt
      481 tgtcggtgag tttggctgct aaaggcataa ttcctcctgt taagaatttc tacagcagca
      541 ggtttcaaaa atccggagaa atattcaacg aaatattaat cacttcaccg gatattctga
      601 tacgtacctt cactggtcta tttaagtaat acaaatt
//
LOCUS       DAZFFG010000379          637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFFG010000379 DAZFFG010000000
VERSION     DAZFFG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.382"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..113)
                     /locus_tag="ACHJ7J_005209"
     CDS             complement(<1..113)
                     /locus_tag="ACHJ7J_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003269.1"
                     /translation="MKRVLFFLLMIFVNFGVIADCEIQAKDHDCFTIFAKGT"
     gene            complement(110..>637)
                     /locus_tag="ACHJ7J_005210"
     CDS             complement(110..>637)
                     /locus_tag="ACHJ7J_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5003270.1"
                     /translation="LKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPT
                     PDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQ
                     DDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGE
                     ILAKMKQRTSRGIRK"
BASE COUNT          165 a          135 c          124 g          213 t
ORIGIN      
        1 gtacccttag cgaaaatagt aaaacaatca tgatctttag cttgtatttc gcaatcagct
       61 ataacaccaa aattaacaaa tatcatcagc aaaaagaaca aaactctttt cattttctta
      121 ttcctctcga tgttctctgt ttcattttag ctaaaatttc acccaaatca tctatccctg
      181 ctgttgctgg cccatcgggt ttattaatgt ctgggaataa atagggttct gcacaagtat
      241 atcctggtgg taattgagtt gttggccaat ttgtctgtgg caggtaatca cttgctgtct
      301 ttgtatccat ccctggatac tggggatcat cctgtggcgt acaagtcaga tttggtttta
      361 cccacttcct gcaatacagt gtgtagtcgt aagggcttaa tgctctgaag aatgttttga
      421 aaacattagg cgcactactg gcctggtcaa taggatatgg cagtcttaat tgtttcgcta
      481 tgtctggcaa tttacttgga tcgggcgtag gcggtttgtt gggtctcgct ccatatccgc
      541 cattatttaa gtgtccatat tttgatgcca gtcccattga atctatatac tgaaccggat
      601 tcaacggata ctgataaaaa ttccatcccc ccttcag
//
LOCUS       DAZFFG010000380          634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFFG010000380 DAZFFG010000000
VERSION     DAZFFG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.383"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>634)
                     /locus_tag="ACHJ7J_005211"
     CDS             complement(<1..>634)
                     /locus_tag="ACHJ7J_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO5003271.1"
                     /translation="RLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNYLLK
                     MYSRQHETGGDAIYCGLYGHHIKNGVTYSPINTEFNEGALLFDFFYKKVRFHIGCLFI
                     RKQLLEDNNLFFDEDLRLGEDLDFIYRLLLTCDMYAVPYYMYKHNYRENSLMNSCRTI
                     THYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLN"
BASE COUNT          203 a          122 c           98 g          211 t
ORIGIN      
        1 attaagtaga acattccata aataacgagt tttatgataa gctctatttt gactcaataa
       61 tgtatgaatt tcttctttcc ggttaccttt gtataactgc atcacagaag agtagattct
      121 ttcgtgcgca aatgactcgt gtcgataatg agtgatggtt ctacatgagt tcattaggga
      181 attttctcta tagttatgct tatacatata atatggaacc gcatacatat cgcatgtaag
      241 tagcagtcga tagataaaat ccagatcttc tcctagtcgt aaatcttcat caaaaaaaag
      301 attattatct tccaggagtt gttttcttat aaacaagcac cctatatgga atctgacctt
      361 tttataaaaa aagtcgaaaa gtaaagcccc ttcattaaac tctgtattta taggtgagta
      421 agtaacacca ttttttatat gatggccata aagcccacaa taaatggcat ctcctcccgt
      481 ctcatgttgt ctggaataca tcttcagcag atagttagga tctatctcat catcatcatc
      541 cataaaacat ataaactgtc cttgcgcctt gcggatacct gtattcctgg cagacgaaac
      601 accgccattt tctttattga taataattaa tcga
//
LOCUS       DAZFFG010000381          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFFG010000381 DAZFFG010000000
VERSION     DAZFFG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.384"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..367
                     /locus_tag="ACHJ7J_005212"
     CDS             <1..367
                     /locus_tag="ACHJ7J_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO5003272.1"
                     /translation="LIYPEELKMADHFEYEEDFETDDFDIKKSEIEINDENDDQMINS
                     YEELPVKIEFVLGKKVMNLYEIDELCAKRIISLLPESEKNIEIRVNGALTGYGELVEV
                     DDKLGVEIHSWLSRNNNVK"
     gene            357..>633
                     /locus_tag="ACHJ7J_005213"
     CDS             357..>633
                     /locus_tag="ACHJ7J_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5003273.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFV"
BASE COUNT          207 a           79 c          138 g          209 t
ORIGIN      
        1 tttaatttat ccagaggagt taaaaatggc tgatcatttt gagtacgagg aagattttga
       61 aacagatgac tttgatatca aaaaaagcga aattgagatt aatgatgaaa atgacgatca
      121 gatgattaat agttatgaag agttgcctgt aaaaattgag tttgttcttg gtaagaaagt
      181 aatgaatcta tatgagatag acgagctctg tgcaaaaaga ataatatctc tgcttcctga
      241 atctgagaaa aatatagaga tacgtgtaaa tggcgcgcta actggctatg gcgaacttgt
      301 tgaagtggat gataaattgg gcgtagaaat tcattcttgg ttatcaagga ataataatgt
      361 caaatagcat ttcgctgata gcgatattat cattatttac actattgccg tttataatag
      421 catccgggac atgttttatt aaattctcga ttgtttttgt tattgtccga aacgcgctgg
      481 gacttcagca agtgccatca aatatgacac ttaatggggt tgctttgctt ttgtcaatgt
      541 tcgtaatgat gccggttggg aaggaaatat ataataacag tcagaatgag aatctttcat
      601 tcaataatgt agcgtcggtt gttaattttg ttg
//
LOCUS       DAZFFG010000382          632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFFG010000382 DAZFFG010000000
VERSION     DAZFFG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.385"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>632)
                     /locus_tag="ACHJ7J_005214"
     CDS             complement(<1..>632)
                     /locus_tag="ACHJ7J_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO5003274.1"
                     /translation="YLKNKIPFENMIIDLSALKKDVLVSLKQCCFKKMTFTGNISYEN
                     LNGPVFENCFFEECNFENVSLVGFDKVTCESYDVLCNVKPNNKIPIYGMFKGCFLYQC
                     EMKNFKIETSKIYSINQDPQRGDKKVGAYLFMQSFVYASNLQDGVCKEASVIASSLLS
                     CNIAKLNGVGMDFIETSFYGKTRGGFRDSNNFYDCDFRYVNMTCKRDGIR"
BASE COUNT          212 a          124 c           76 g          220 t
ORIGIN      
        1 cacgaattcc atctcgtttg catgtcatat taacataacg aaaatcacaa tcataaaaat
       61 tattactatc tctaaaacca ccgcgagttt ttccataaaa cgaagtttct atgaaatcca
      121 ttccaacacc attaagcttt gctatattgc aagataataa actgcttgca ataacacttg
      181 cttctttaca cactccatct tgaagattac ttgcatatac aaatgactgc ataaacagat
      241 atgcaccgac tttcttatcc cctctttggg gatcttgatt tatagagtat atttttgatg
      301 tctctatctt gaaatttttc atttcgcatt gatacaaaaa acacccttta aacataccgt
      361 aaatcggtat tttattattt ggttttacgt tacaaagaac gtcatagctt tcacaggtca
      421 ctttatcaaa gccaacaagg gacacattct cgaaattaca ttcctcaaaa aaacaatttt
      481 caaatactgg gccgttaaga ttttcgtaag atatattacc tgtgaatgtc atctttttaa
      541 agcaacactg ttttagcgat accagaacat cttttttcag tgcagataaa tcaattatca
      601 tattttcaaa ggggatttta ttttttaaat at
//
LOCUS       DAZFFG010000383          620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFFG010000383 DAZFFG010000000
VERSION     DAZFFG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.386"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          215 a          123 c           76 g          206 t
ORIGIN      
        1 actatacaca acttcctgaa taaaataaat attcgtttta tcaccttcga taacatcttc
       61 cattaatccc tccagacaca accaaattac atggcaagag tataattcta atgccactca
      121 ccagattgaa tacttgactt aatattatca cttgatcaaa caatcataat gaatttcatc
      181 tttattgtct ttttaaaagt gatagctaag ctcgttttat tcattaaacc agatgatgca
      241 atactttgga tcaaataaat attcccacta agatgaatgt tcattttgct ttcataagcc
      301 atacgcttgc cctgctgcaa ggactaatcg aaagtggaac attatttcac gcagtcattt
      361 acatacgatg gcaaaccgtt tttatttgat tgcatctccc taatcaaata catatttatt
      421 tctctactac taactagttc agccagttat cacatcacat ttgagcatta taattcccca
      481 aaaacattca tctattaatg gcgaatgaaa tagataatga attaaaataa tcatctagta
      541 aaaattgttt gataccggga tcataagccc gcataacaga gtgttttgtt tccaataatg
      601 ggataatagt attcactcga
//
LOCUS       DAZFFG010000384          616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFFG010000384 DAZFFG010000000
VERSION     DAZFFG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.387"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>616
                     /gene="espY4"
                     /locus_tag="ACHJ7J_005215"
     CDS             <1..>616
                     /gene="espY4"
                     /locus_tag="ACHJ7J_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector EspY4"
                     /protein_id="HFO5003275.1"
                     /translation="LEGPVFENCYFDDCKFNNIQLYETNDDTVGSGNKKPIKGMFKGC
                     FISKCKIENYRCETSKIYTVTQPDSIQEKLGSYLFMQSFVQDCIIQGGCCPGSSILLS
                     HFYNCNIIGLDAHGMDFLANSFNKSNYDEIRGPDTGTVFYNCNLKHIKINDGLREDGS
                     DRSMFNPKEYFSDYIERRSRNLKLDDNFSKCVESIKKGTDSSFS"
BASE COUNT          205 a           80 c          125 g          206 t
ORIGIN      
        1 accttgaagg tccggttttt gaaaattgct atttcgatga ttgtaaattc aacaacatac
       61 agctttatga gactaatgat gatactgtgg ggtcaggaaa taaaaaacca ataaaaggta
      121 tgtttaaagg ttgttttatt tctaaatgca aaattgaaaa ttatagatgt gagacgtcta
      181 aaatctacac cgttacacaa cctgatagta ttcaagaaaa attaggttct tatctcttta
      241 tgcagtcttt cgttcaggat tgtataatcc agggaggatg ttgtcctggc tcgagtatac
      301 ttctgagtca tttttataac tgtaatataa tagggcttga tgctcatgga atggattttt
      361 tggcaaattc gttcaacaag tctaattatg atgaaatcag gggtccggat acggggactg
      421 tgttttataa ttgtaatttg aagcatatta aaataaacga tggtcttcgt gaagatggtt
      481 cagatagatc tatgtttaac cccaaagagt atttttctga ttacattgaa agaagatcga
      541 gaaatcttaa gttagatgat aatttttcga aatgcgtaga gagtataaag aaagggaccg
      601 attcttcatt tagcaa
//
LOCUS       DAZFFG010000385          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFFG010000385 DAZFFG010000000
VERSION     DAZFFG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.388"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          190 a          120 c          121 g          183 t
ORIGIN      
        1 tctatatatg taaataatgg cgagttaacg tatcacctga cacgcttcta tccaaaatta
       61 agaagtattt gactactcaa gccaataaat gtgtatagaa tgattatacg ggatagttac
      121 aagttcaccg atacggtgca ggactcaatg gcgatgtctg tgggagcgtt tgtgtcacga
      181 tatgatggac gtcctttctt aatgcagtga gtgccatact tttactgact gcattaatcc
      241 gagagtaaca tcccatgaac acgatcagga ttgttggtat cgacattgcc aggcctgttt
      301 ttcaggccta cgtctggatt aatgatgatt ctgttgcata gaatagaaac agctcatgct
      361 tcaaattgca ggatatcgtt caacgattcg aatccggaac actattcgct atggaggcat
      421 gttcaacttc tcgttctggg gaatgaacgt tcaggactat ggaatatctc ctgactattc
      481 cctgctcaac agcatcattt ttggttaaaa aataacatta tccttaatat atagaaaagg
      541 aactcgtcct ggacaagtcc tattaaaaat gcatatcata ttcacctcat cggaaaacga
      601 ttaaaaatca gtaa
//
LOCUS       DAZFFG010000386          613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFFG010000386 DAZFFG010000000
VERSION     DAZFFG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.389"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(1..>613)
                     /locus_tag="ACHJ7J_005216"
     CDS             complement(1..>613)
                     /locus_tag="ACHJ7J_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO5003276.1"
                     /translation="GMVLSAIALSFHLWPGFHNPVVLQSVTVGPKSTPYTMYFNFDKA
                     LVPFLLVLCTASLFEKEIKSEVSLWMWGGLFLSVPLILFLAVFLGGLNPEIHFPEWLP
                     EFILANLFFVSLAEESLFRGYIQSRLSEVMSPLVALVVAALLFGLFHYSGGTLLMLFA
                     TLSGIVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPLLKHH"
BASE COUNT          252 a          136 c           99 g          126 t
ORIGIN      
        1 tcaatgatgt tttaataatg gataggtaaa aaataacaag tgacacagat tcaacccaaa
       61 atgaaaaagg gtggcaaccc acaaacgccc actccacatc catgacaatc catacacaat
      121 accagataac gtggcaaata acataagtaa agtaccacct gaataatgaa aaagaccaaa
      181 cagcaaagct gccacaacta atgcaaccaa tggggacatt acttctgata accgtgactg
      241 aatatatcct ctaaacaatg actcctctgc cagagataca aaaaacaaat tagccaatat
      301 aaactctggc aaccactcag ggaaatgaat ttctggattt aacccaccca aaaacacagc
      361 caaaaataag ataagaggaa cagagagaaa gagacccccc cacatccaca aagacacttc
      421 tgattttatt tctttttcaa acaaagaggc tgtacacagg actaacaaaa atggcactag
      481 cgccttatca aaattaaaat acattgtata tggtgtgctt ttaggtccga cagtaacaga
      541 ctgtagcact acgggattgt gaaaaccagg ccataaatgg aaagataatg ctatggctga
      601 taaaaccata cca
//
LOCUS       DAZFFG010000387          596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFFG010000387 DAZFFG010000000
VERSION     DAZFFG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.390"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>596
                     /locus_tag="ACHJ7J_005217"
     CDS             <1..>596
                     /locus_tag="ACHJ7J_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFO5003277.1"
                     /translation="AQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNIPDNATVQSVV
                     DDLSHAGQIHFTSALTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGR
                     ATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNMLQAGAFNYSLN
                     RDSDESWYLRSEERYRAEVPLYASMLIQAMDYDRILAGS"
BASE COUNT          138 a          169 c          177 g          112 t
ORIGIN      
        1 gctcagcgcg gcacggccct gaagctgacc ggcagcacag tgctgaacgg tgccattgac
       61 cccacgaatg tcaccctgac atccggtgcc acctggaata tccccgataa cgccacggtt
      121 cagtcggtgg tggatgacct cagccatgcc ggacagattc atttcacctc cgccctcaca
      181 gggaagttcg ttccgacaac tctgcaggtg aaaaacctga acgggcagaa tggcaccatc
      241 agcctgcgtg tacgcccgga tatggcgcag aacaatgctg acagactggt cattgacggt
      301 ggcagggcaa ccggaaaaac catcctgaat ctggtgaacg ccggcaacag tggtacgggg
      361 ctggcgacca ccggtaaggg tattcaggtg gttgaggcca ttaacggtgc caccacggag
      421 gaaggggctt ttgtccaggg gaatatgctg caggccgggg cctttaacta ctccctcaac
      481 cgggacagtg atgagagctg gtatctgcgc agtgaagaac gttatcgtgc tgaagtcccc
      541 ctgtacgcct ccatgctgat acaggcaatg gactatgacc ggattctggc aggctc
//
LOCUS       DAZFFG010000388          596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFFG010000388 DAZFFG010000000
VERSION     DAZFFG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.391"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>596
                     /locus_tag="ACHJ7J_005218"
     CDS             <1..>596
                     /locus_tag="ACHJ7J_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFO5003278.1"
                     /translation="AQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVV
                     DDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGR
                     ATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLN
                     RDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGS"
BASE COUNT          141 a          172 c          177 g          106 t
ORIGIN      
        1 gctcagcgcg gtacagccct gaagctgacc ggcagcactg tgctgaacgg tgccattgac
       61 cccacgaatg tcactctcgc ctccggtgcc acctggaata tccctgataa cgccacggtg
      121 cagtcggtgg tggatgacct cagccatgcc ggacagattc atttcacctc cacccgcaca
      181 gggaagttcg taccggcaac cctgaaagtg aaaaacctga acgggcagaa tggcaccatc
      241 agcctgcgtg tacgcccgga tatggcacag aacaatgctg acagactggt cattgacggc
      301 ggcagggcaa ccggaaaaac catcctgaac ctggtgaacg ccggcaacag tgcgtcgggg
      361 ctggcgacca gcggtaaggg tattcaggtg gtggaagcca ttaacggtgc caccacggag
      421 gaaggggcct ttgtccaggg gaacaggctg caggccgggg cctttaacta ctccctcaac
      481 cgggacagtg atgagagctg gtatctgcgc agtgaaaatg cttatcgtgc agaagtcccc
      541 ctgtatgcct ccatgctgac acaggcaatg gactatgacc ggattctggc aggctc
//
LOCUS       DAZFFG010000389          595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFFG010000389 DAZFFG010000000
VERSION     DAZFFG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.392"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>595
                     /locus_tag="ACHJ7J_005219"
     CDS             <1..>595
                     /locus_tag="ACHJ7J_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003279.1"
                     /translation="IFDSPDASSIFDTQGYLSTYHVQPDLLDYKNKIIILLVKFLNHI
                     NEANSKYKTAEKKDFYNKLLAPLRYINNDGNKILILNYLITNIFTSTNSNPDGLLFDA
                     ILDIAHNSVHRGYLNQHSLIRHRCVQHALINIISKQTDIDLKIKWIKDIVLVLKLQHD
                     DLSKCISPLIFAIESIKTTSINDTTIKTSSQILKLHN"
BASE COUNT          224 a          109 c           79 g          183 t
ORIGIN      
        1 tgatttttga tagtcccgat gcatcttcta tttttgatac ccagggatac ctctcgacgt
       61 atcatgttca gccagattta ctcgattata agaacaaaat aatcatcctt ttggttaaat
      121 ttcttaatca tattaatgag gcaaatagca aatacaaaac tgcggaaaag aaagatttct
      181 ataacaagtt attggcacca ctacgctata taaataacga cggaaataaa atattgatat
      241 taaattatct tataacgaac atctttacat ccacaaattc aaatcctgat ggtttgctct
      301 ttgatgctat actggatatt gcacataact ccgttcacag aggatattta aatcaacata
      361 gtttaatcag gcatcgctgc gtccaacacg cactgattaa catcatctca aaacaaacag
      421 atattgactt aaaaataaaa tggataaaag atatagtttt agtgttgaaa ttacaacatg
      481 atgatttatc gaaatgtatc agcccactta ttttcgctat tgaatcgata aaaacaacca
      541 gcattaacga cacgacaatt aaaacctctt cgcaaatatt aaaactgcat aatta
//
LOCUS       DAZFFG010000390          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFFG010000390 DAZFFG010000000
VERSION     DAZFFG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.393"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            42..581
                     /gene="elfA"
                     /locus_tag="ACHJ7J_005220"
     CDS             42..581
                     /gene="elfA"
                     /locus_tag="ACHJ7J_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5003280.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
BASE COUNT          183 a          111 c          135 g          164 t
ORIGIN      
        1 aaaggcatta tcatatttac gaagagatca ggatgataac gatgaaaaaa agtgtattga
       61 cggcgtttat aactgtggta tgtgcaacgt ccagcgttat ggctgctgat gataatgcta
      121 tcacggatgg ctcagtaaca tttaatggta aagttattgc tccagcttgt accctggtag
      181 ctgcgacgaa agattccgtg gtgactttgc cagatgttag tgccacgaag ttgcaaacca
      241 atggtcaggt ttctggcgtg caaactgatg tgccaattga attaaaagat tgtgatacta
      301 ccgtaacaaa aaatgcaacg ttcaccttta atggcactgc ggatactact cagattacag
      361 cgtttgctaa ccaggcctca tctgatgctg ctacaaacgt ggccctgcaa atgtatatga
      421 atgatggtac aacggccatc acgccagaca cagaaaccgg gaacattttg ttgcaagatg
      481 gagatcagac gttgactttt aaagttgatt atatcgctac ggggaaagcg acttctggta
      541 atgtgaatgc ggtaacaaat ttccatatta actattatta atagaactca tta
//
LOCUS       DAZFFG010000391          591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFFG010000391 DAZFFG010000000
VERSION     DAZFFG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.394"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          168 a          105 c          129 g          189 t
ORIGIN      
        1 atgaaaaata tgtacgggga agtatttacg tacgtaaaaa atcaggtata ctgctaaaaa
       61 tatttatatt gctgcatagt ttgaacccgc ctccgagagg ggctttatat gcgtgcgccg
      121 tgactggcgc tgttcttcaa ctgtgatggc tgaagagcgg ttaaggcgga gcagcagggt
      181 aaggttgcct gtatcaccaa agccgatact cacactgggc gctatgacct tgcgagccat
      241 acgggatgta cacagaaaga aaggtacgac cgcaattaat aacaaaatct taaaaatcgc
      301 atatggcact attatttttc taaatattgt atattttagc attgcgggat atccctgtaa
      361 cggagttgca taatagcatt ttgctctacg actttgccag tctccagcag tggctggctt
      421 ttttatgatc gtaatatcca gcgtctcaat aaatgttgtt gtcagagtac gttaagttgt
      481 agcaagatat ctgacaagat atgttaatgt tgcagattac ttttaacacg attatcattt
      541 ctttatgcgt tgatgaataa actgtattta tcttttttat gcttgcgttt t
//
LOCUS       DAZFFG010000392          590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFFG010000392 DAZFFG010000000
VERSION     DAZFFG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.395"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>590)
                     /locus_tag="ACHJ7J_005221"
     CDS             complement(<1..>590)
                     /locus_tag="ACHJ7J_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5003281.1"
                     /translation="DESVREYHSRNLLKKPDYINSIRVFFRKWFNLELPGVSENQVKC
                     LYQGKPKRRELGKAVKVKDTKKGPFSDDEVITFNEGAMWLYDSGEITLDELAMALITS
                     YTGRRPIQTSHLKLKDIVSLFQNVGTDYSINFPRAKHSGVFRSEFTKLKITEDLHDII
                     LILGERSINTVENYLGRCVDNYELKEIPLFIDVIAL"
BASE COUNT          183 a          126 c           74 g          207 t
ORIGIN      
        1 tcaagcgcaa taacatcaat aaataaaggt atttccttta actcataatt atcaacacat
       61 ctgccaagat agttttcaac agtgttaata cttctctcac ccaatatcaa gataatatcg
      121 tgcaagtctt cagttatttt caactttgta aactcagaac gaaatactcc actatgtttt
      181 gctcttggaa aatttattga ataatcggtt cctacattct ggaatagact cacgatatct
      241 ttcaatttca agtgtgaggt ctgtataggt cgcctccccg tgtatgaagt tatcagcgcc
      301 attgccaatt catcgagtgt tatttcacca gaatcatata accacatggc accttcatta
      361 aaggtaatta cctcatcatc actaaatggt ccttttttag tatctttaac ttttaccgct
      421 ttaccaagtt cccttctttt aggtttgcct tgatataaac atttcacttg attttcagag
      481 acacctggaa gttctaaatt aaaccactta cggaaaaaaa ctcttatact attaatataa
      541 tccggctttt ttaataaatt cctactatgg tattctctaa cagactcatc
//
LOCUS       DAZFFG010000393          587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFFG010000393 DAZFFG010000000
VERSION     DAZFFG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.396"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            1..587
                     /locus_tag="ACHJ7J_005222"
                     /pseudo
     CDS             1..587
                     /locus_tag="ACHJ7J_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484288.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          187 a          107 c          112 g          181 t
ORIGIN      
        1 gtttatatcg actaatattg cagagtttct agacaacttt acgaaaatta cagattccag
       61 actttgcaaa ggtttcagtg actgggctaa ttcagtcaaa gaaggcgcat cgaatgactt
      121 caaaaaaaat gtggatatag cattggtgag attgtttaaa tgtgttgaac tccacaatag
      181 taaacttgac ttaacagatc ttcacttggg gtctttgcca cctcttccat cctggattga
      241 ggtgctttat cttcgtcata atggccttgc cacaatacag gttcccaaat tctgcaaaga
      301 attagagctc gatttcaata attatatggt atttccaaaa gtatccgatg gaattaccca
      361 ggtctccgta gataataatc tgatatcgcg tgttgactca tctccgtcaa aggctatgac
      421 aatttctatt tatcgcaata aaatatggta aataccagca atgcctgaga ctactaagct
      481 gtttaattgt ggcgataata agataaaaga aattcaatac ttcccaaatc atctggagaa
      541 agcacgtatg gagtgtgata ttgaggttgt ctctgcgata tatagca
//
LOCUS       DAZFFG010000394          586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFFG010000394 DAZFFG010000000
VERSION     DAZFFG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.397"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            12..503
                     /gene="ygeG"
                     /locus_tag="ACHJ7J_005223"
     CDS             12..503
                     /gene="ygeG"
                     /locus_tag="ACHJ7J_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5003282.1"
                     /translation="MNKETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDGIY
                     AYAFDYHEKGNITDAEIYYKLLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFFHIINNCEDDSVKSKAQAYVELLTDNSE
                     DND"
BASE COUNT          222 a           91 c           97 g          176 t
ORIGIN      
        1 agaggaatat tatgaacaaa gaaacaattg aaatattcaa taatagtgat gaatgggcaa
       61 atcaactaaa acacgcatta tcgaaaggag aaaacctggc attactgcat ggtttaactc
      121 ctgatatcct tgatggaata tatgcatatg cattcgacta ccatgaaaag ggtaatataa
      181 cggacgcaga aatttattat aaattattgt gcatttatgc gttcgaaaat catgaatatc
      241 taaaaggttt tgcatcagta tgtcagtcaa aaaagaaata tcaacaagca tatgaccttt
      301 acaaactaag ttacaattac tccccatatg atgactattc agttatttat cgtatgggtc
      361 aatgtcaaat tggcgcaaaa aatatcgata acgcaatgca atgtttcttt catataatta
      421 acaattgtga ggatgatagt gttaagagta aagcgcaggc atatgtcgaa ctcttaaccg
      481 ataattcaga agataatgat taagcattac aatcatttgg ttaaaattgc tggttatagt
      541 gcagttaacc actataacca gtacctctta tcttttctta aaccaa
//
LOCUS       DAZFFG010000395          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFFG010000395 DAZFFG010000000
VERSION     DAZFFG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.398"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..553)
                     /locus_tag="ACHJ7J_005224"
     CDS             complement(<1..553)
                     /locus_tag="ACHJ7J_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5003283.1"
                     /translation="MIYIINDEVRFNSSSNKLTSLLTSEEVILTYPAGKCLKLLLDNA
                     YSVVSHNQFFEKVWSDSSAEVATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGF
                     KINEEINITVLDNNHNENESVESEENDTHTENINKNHVAKGIFTTKNTLLIVCFFAAW
                     GIVYISSYFFDMAFQSKSHTIKKI"
BASE COUNT          169 a           97 c           96 g          216 t
ORIGIN      
        1 ctattttttt tatagtgtga gatttgcttt gaaaagccat atcgaaaaaa taagaagata
       61 tatacactat cccccatgcc gcaaaaaaac aaactataag taatgtattc tttgtggtaa
      121 atataccttt tgcaacgtga tttttattta tattttcagt atgggtgtca ttctcttcac
      181 tctcaacact ttcattctca ttgtgattat tgtcgagaac agttatgttg atttcttcat
      241 tgattttgaa gcctttacgt ggcaccgttc tgataacatc attaaatatc gtcgctgagc
      301 tgatttcacg aaaggaacgc cttatcatcg atatattttg atataaagtg ttggttgcga
      361 cttcggcact gctatcgctc cagacttttt caaagaattg attgtgtgac acgacactat
      421 aagcgttatc taaaagtaac tttaagcatt ttcccgcagg ataagtgagt atcacttcct
      481 ctgatgtaag gagtgatgtt aacttattag atgatgagtt gaatcttact tcatcattta
      541 taatgtagat cattacattt tataccttag ttgtgtat
//
LOCUS       DAZFFG010000396          576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFFG010000396 DAZFFG010000000
VERSION     DAZFFG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.399"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>576
                     /locus_tag="ACHJ7J_005225"
     rRNA            <1..>576
                     /locus_tag="ACHJ7J_005225"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          162 a          125 c          177 g          112 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatg
//
LOCUS       DAZFFG010000397          572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFFG010000397 DAZFFG010000000
VERSION     DAZFFG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.400"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(64..432)
                     /locus_tag="ACHJ7J_005226"
     CDS             complement(64..432)
                     /locus_tag="ACHJ7J_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5003284.1"
                     /translation="MSDTLSGTTHPDDNNSHPWWGLPCTVTPCFGARLVQESNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSRGYVYMAVYPTPETKK"
BASE COUNT          133 a          141 c          168 g          130 t
ORIGIN      
        1 atgagaaatg gggaagtggc gtggtgaaaa caccaagccg gagcagaagg ttattctgat
       61 gatttacttt ttcgtttcgg gcgtcggata aacagccata taaacgtaac ctcgactgcc
      121 gagggtatcg gctttgcagg tcagcccttt tgcatacagc gtgacggtat gctgatggcg
      181 gggattcagc tcaccgctgg tgagcatgag ttccagttgt ttcatcagta gcggaaaggc
      241 ctggtccaga tggtacgcat ccgcgtcgct gaaccggcct ctgataccgg cgcggtcggc
      301 aaggtaatgc aaccggttac tctcctgcac cagacgtgcc ccgaaacagg gcgtcacggt
      361 gcagggcagc ccccaccagg ggtggctgtt gttgtcatcg ggatgagttg tcccggagag
      421 tgtgtctgac acgataaaat cctcacaaat aatcagttaa gaatggtccg gtagcggcga
      481 taattctgtt catcagaatg ccagtcctga ttcagtacag ggtgacgctc atcagccgga
      541 catacgggcc aaaaactgtc cttacggcgt tc
//
LOCUS       DAZFFG010000398          570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFFG010000398 DAZFFG010000000
VERSION     DAZFFG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.401"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>570
                     /gene="eilA"
                     /locus_tag="ACHJ7J_005227"
     CDS             <1..>570
                     /gene="eilA"
                     /locus_tag="ACHJ7J_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFO5003285.1"
                     /translation="EYHIPPKELGVIILLLNADGEIVSKEEIIDKVWSASVASDESLT
                     RCIYALRKLLHENKQCKYIETVYGRGYRFTVPIVIVTDNEPVKSTTTLAVFPFRTEGS
                     INVLKLHYELVQGLSKYAFCGLDILPASVTNEASDFSTIHQFINKTGPEYYIMGQVVH
                     YGKNWRLFIELIHARTHKLIDHQSIDFNPD"
BASE COUNT          166 a          110 c          112 g          182 t
ORIGIN      
        1 gaatatcata ttccacccaa agaacttggc gttatcattc tattacttaa tgctgacggt
       61 gaaattgtta gcaaagaaga gattatcgat aaggtctgga gcgcgagtgt tgctagtgat
      121 gaatctttaa cacggtgtat ttatgcattg cgtaaacttt tgcatgagaa taaacagtgt
      181 aagtatatcg aaacggtcta tggtcgtgga taccgtttta ctgtccccat tgtgattgta
      241 accgataatg aaccggtaaa gtcgaccacc actcttgccg tttttccctt tcgtactgaa
      301 ggttcgatca atgttctaaa actccattac gaactggtcc agggcctttc aaaatatgct
      361 ttttgtggtt tagatatttt acccgcgtcg gtaacgaatg aagccagtga tttctccact
      421 atccatcagt ttatcaataa aacaggccca gaatattata ttatgggtca ggtcgttcac
      481 tatggtaaaa actggcgttt atttattgaa ttaatccatg ccagaacgca taaattgatt
      541 gatcatcaaa gtattgattt caaccccgac
//
LOCUS       DAZFFG010000399          569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFFG010000399 DAZFFG010000000
VERSION     DAZFFG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.402"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            74..>569
                     /locus_tag="ACHJ7J_005228"
     CDS             74..>569
                     /locus_tag="ACHJ7J_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFO5003286.1"
                     /translation="MLVSKSNGFNASAVLGSGSYNENKSSKHMELLAHSIVKLICKEA
                     ASATYRGALETLQKMMSECKYKEGNAFVIMGAGGQLKRIKYDVDENKLKIFTIYFDNN
                     YEVVTDGEPDVVCLSKQVWENLLIKLKLDNKENTASEVHKSANKGEVEQLVECSKRNE
                     KTLLD"
BASE COUNT          215 a           73 c          120 g          161 t
ORIGIN      
        1 gagcgatttt ttatagtgac aaacagtaat aacattaaaa atagccatgt agacattaat
       61 tgaggaatag acaatgttag ttagtaaaag caacggattt aacgctagcg cagttttggg
      121 tagtggaagt tataatgaaa ataaatcttc taaacatatg gagctactag ctcatagtat
      181 tgtaaaatta atttgtaagg aagctgcatc agcgacgtat cgcggtgctc ttgaaacttt
      241 acaaaaaatg atgtctgaat gtaaatataa agaaggcaac gcctttgtca ttatgggagc
      301 tggagggcaa ttaaaacgta ttaaatatga tgttgatgaa aacaaattaa aaatattcac
      361 catatacttt gataataatt acgaggtagt tactgatggt gagcctgacg tagtatgttt
      421 aagcaagcag gtctgggaaa atcttctcat taaactaaag ctggataaca aagaaaacac
      481 ggcttctgaa gttcataaat cagcgaataa aggtgaggtt gagcaattag ttgaatgctc
      541 taagagaaat gaaaagacgc ttcttgata
//
LOCUS       DAZFFG010000400          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFFG010000400 DAZFFG010000000
VERSION     DAZFFG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.403"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..202)
                     /locus_tag="ACHJ7J_005229"
     CDS             complement(<1..202)
                     /locus_tag="ACHJ7J_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertussis toxin-like subunit ArtA"
                     /protein_id="HFO5003287.1"
                     /translation="MRIVLILLFTLWSFGSYAVQFVYRIDSRPPDVVFRDGFRSHGNN
                     RNLQQHIRGDSCAAGSRDSAFIA"
     gene            complement(221..>566)
                     /locus_tag="ACHJ7J_005230"
     CDS             complement(221..>566)
                     /locus_tag="ACHJ7J_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subtilase family AB5 toxin binding subunit"
                     /protein_id="HFO5003288.1"
                     /translation="GAKKDGMYSDVTIRDIHTGVVDSKPYFCVEVVKSGLDTKACLVL
                     GYSIWSNAFDTFYNQAMYYYSTGQKIRLYYEPNTWTHPDFSKITTNAIAGFSTCSSDD
                     YCFGPDRKKGSN"
BASE COUNT          204 a          116 c           79 g          167 t
ORIGIN      
        1 tagcaatgaa tgcgctatcc cttgatccag cagcacatga atcaccacgg atatgctgct
       61 gcaaattcct gttgttacca tgagatctaa aaccatctct aaaaacaaca tcaggaggtc
      121 ttgaatcaat acgatataca aattgtacag catatgaacc aaaagaccat aaagtaaata
      181 acaatatcaa tactattctc atataacgat ctctataatc ttaattactt cctttctttc
      241 tatcaggacc aaaacaataa tcgtctgaag agcaggttga aaatccagct attgcattag
      301 ttgttatttt tgaaaagtca gggtgtgtcc atgtgtttgg ctcataatat aaccgtatct
      361 tttgtccagt agaataatag tacatagctt gattatagaa cgtatcaaac gcattagacc
      421 aaatcgaata tcctaaaact aaacaagctt tcgtatcaag accagatttg acaacctcaa
      481 cacagaaata tggtttagaa tcaacaactc cagtatgaat atctctaata gttacatcag
      541 aatacattcc atccttttta gcacct
//
LOCUS       DAZFFG010000401          562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFFG010000401 DAZFFG010000000
VERSION     DAZFFG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.404"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>562
                     /locus_tag="ACHJ7J_005231"
     CDS             <1..>562
                     /locus_tag="ACHJ7J_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO5003289.1"
                     /translation="FYRNEKIECACDFMMAKDAQGYIDLSELDLTSCHFKGDVISKVS
                     FISSNLQHVTFECKEIGDCNFTTAIVDNVIFKCRRLHNVIFIKASGDYVDFSKNILDT
                     VDFSQSQLTHSNFRECQIRNSNFDHCYLYASHFTRAEFLTDKEISFIKSNLTAVMFDH
                     VRISTGNFKDSVTQLMVLSIDYSDIFG"
BASE COUNT          185 a           80 c          100 g          197 t
ORIGIN      
        1 aattttatag aaatgaaaaa atagaatgcg cttgtgattt tatgatggct aaagatgcgc
       61 aggggtatat cgacctgtct gaattggatt taacaagttg tcattttaaa ggtgacgtta
      121 tttcgaaggt gtctttcata tcatcaaatc tacaacatgt aacattcgaa tgtaaagaaa
      181 ttggggattg caattttact actgcaatag ttgataatgt catatttaaa tgtcgacgtt
      241 tacacaatgt aatttttatc aaagcgagtg gtgattatgt cgattttagc aaaaatattc
      301 ttgatacagt tgacttctcg cagagtcaac ttactcatag taattttcgt gaatgccaga
      361 ttagaaattc aaacttcgat cattgttatc tttatgcttc gcacttcacc agagcagaat
      421 ttcttactga caaagaaata tcatttatta aatcgaattt aacagctgtt atgtttgatc
      481 atgtgcgaat atcgacaggg aattttaaag acagcgttac acaactaatg gtattatcta
      541 ttgattactc agatatattt gg
//
LOCUS       DAZFFG010000402          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFFG010000402 DAZFFG010000000
VERSION     DAZFFG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.405"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(61..225)
                     /locus_tag="ACHJ7J_005232"
                     /pseudo
     CDS             complement(61..225)
                     /locus_tag="ACHJ7J_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505217.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(299..>550)
                     /locus_tag="ACHJ7J_005233"
     CDS             complement(299..>550)
                     /locus_tag="ACHJ7J_005233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5003290.1"
                     /translation="VKDAATGIININSGTGMASGVGIQVASGNSITNTPAKFNIATEM
                     TPESNTSQKIPLVARLIQTDTNVTPGKIESVITFTINYY"
BASE COUNT          170 a          106 c           98 g          176 t
ORIGIN      
        1 ataccttgta aaaaatcctg ataaagcaaa tcctaaatca ccacgaggaa atccccgcta
       61 gaaaagatat ggcattgcca gtgtgagatt tttgtacaag ataatacgca gcataaccga
      121 aacaattcct ataaaaattt gccaacgtaa cagacgataa agtggggcaa tgtctgcatt
      181 gcataaaaat ttatgtggtg ctgttcctaa acatatatcg cacatctatg ttagagctga
      241 cactctttta tatttgaaat ggttcatatt taccacctcc catcatgaaa cagttgtgtt
      301 aataataatt aattgtaaaa gttataactg actctatttt ccctggtgta acgttagtgt
      361 ctgtttgtat caatcgtgca accaatggaa ttttttgaga agtattagac tccggggtca
      421 tctcagttgc gatattaaat ttggccggtg tgtttgttat actattgcct gaggctacct
      481 gaattccgac tcccgatgcc atgcctgtgc cactgttaat attaattata cctgtagcag
      541 catctttaac
//
LOCUS       DAZFFG010000403          543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFFG010000403 DAZFFG010000000
VERSION     DAZFFG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.406"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            145..>543
                     /locus_tag="ACHJ7J_005234"
     CDS             145..>543
                     /locus_tag="ACHJ7J_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5003291.1"
                     /translation="MNSQNALSNMRLAVRGEISCHSTLVLKKIRFYNCAIIYLRNAQL
                     LVKTIDGGVINIPPESICYIEKNTVIDVTLNVLGKGMPYDIFHIDNNILSCICKVMEP
                     LLLNPHKVGSMRSRIFNCTADKTDTEIFKII"
BASE COUNT          180 a           78 c          103 g          182 t
ORIGIN      
        1 gactaactat ttatagtcta tgaaattagt tgatatgtcg atgcgaaact ttttaccggg
       61 aagtattcat ctttgcgtta tttccgagtt tacagagtgg atatcccatg aaactatatt
      121 gttacaaagc atgggggtga ctgtatgaat agtcaaaatg ctttaagtaa tatgaggctt
      181 gctgttagag gagagatatc ctgtcactct acattggttc ttaaaaagat tagattctac
      241 aattgtgcta ttatttatct tcgcaatgcc caattactgg ttaaaacaat agatgggggg
      301 gttattaata ttcctcctga atcaatatgt tatattgaga aaaatacagt cattgatgtt
      361 acacttaacg tgttaggtaa aggcatgcct tatgatattt tccacataga caacaatata
      421 ttaagctgta tctgcaaagt catggaacca ttgttactga atccacataa agttggttct
      481 atgcgaagta gaatttttaa ttgcacggcg gataagacag atacagaaat atttaaaata
      541 ata
//
LOCUS       DAZFFG010000404          542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFFG010000404 DAZFFG010000000
VERSION     DAZFFG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.407"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>542
                     /locus_tag="ACHJ7J_005235"
     CDS             <1..>542
                     /locus_tag="ACHJ7J_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO5003292.1"
                     /translation="DLSSTSDISSVCYAFPVGKKIMLVSRHYLPEFQLQNPANKNRAI
                     YRQWVKAGWIRTTPGDCIDYDRIRDDIMADAENFNIRLVGFDTWNATHLRTQLQGAGF
                     EVEPFPQTYLRFSPAAKSFEVFVNRKVIVHRGDPVLAWSMSNVVMQSDANANIKPNKK
                     KSSNKIDPSVAALMAFGTFQA"
BASE COUNT          137 a          133 c          149 g          123 t
ORIGIN      
        1 gacttatcat caaccagcga tatttccagc gtgtgctatg cctttccggt cggtaaaaag
       61 attatgctgg tttcccgtca ctatctgccg gaatttcagc tacagaaccc cgccaataaa
      121 aaccgcgcca tctatcgcca gtgggtaaag gcgggctgga tacgcacaac accgggtgac
      181 tgcattgatt atgaccgtat ccgtgatgac atcatggcgg atgcagagaa tttcaatatc
      241 aggctggtgg gcttcgatac atggaacgcc acgcacctga ggacgcagct acagggggca
      301 ggatttgagg tggagccgtt cccgcaaaca taccttcgtt tcagtccggc ggcgaaatcg
      361 ttcgaagttt ttgttaaccg gaaggtgatt gtgcatcgtg gcgatccggt gctggcctgg
      421 tcaatgagta atgttgtgat gcagagtgac gcgaacgcca atatcaagcc gaacaagaaa
      481 aaatcatcca acaagataga cccgagcgtt gcggcgctga tggcgtttgg cacattccag
      541 gc
//
LOCUS       DAZFFG010000405          539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFFG010000405 DAZFFG010000000
VERSION     DAZFFG010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.408"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>539
                     /locus_tag="ACHJ7J_005236"
     CDS             <1..>539
                     /locus_tag="ACHJ7J_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO5003293.1"
                     /translation="HKVIHPKTNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVY
                     SALELGMGARPEGLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNESMFVLIYELDD
                     ESEVDDPAMWIKANPNIDVSVDREKLASTIQKARGIPSQWVEMLTKRFNIWCQGATPW
                     MGNGAWAECAGTFAEADLYG"
BASE COUNT          139 a          125 c          159 g          116 t
ORIGIN      
        1 cacaaggtga tacacccgaa aaccaacagc ctgttaaagc cactggcagc aaaagcggca
       61 accattgaag gtacaaaccc gagtcttgcc attgtggatg aatatcacct gcaccctgac
      121 aacggtgttt attccgcgct tgaactggga atgggcgcgc gtccggaggg gttattattt
      181 gccatcacca catcggggag caacgttgtt tcagcctgta aacaacacta cgactattgc
      241 tgccagatac tggatggtga agaggtgaac gaatccatgt tcgtgctgat ttacgagctg
      301 gatgatgaaa gcgaggttga cgatccggcg atgtggataa aggcgaatcc caatatcgat
      361 gtttccgtcg atcgtgaaaa actggcctca accatccaga aagcgcgggg tattccgtcg
      421 cagtgggtgg aaatgctcac caagcgattc aatatctggt gtcagggggc tacgccgtgg
      481 atgggtaacg gtgcatgggc ggagtgcgcc ggaacgttcg cggaggcgga tttatacgg
//
LOCUS       DAZFFG010000406          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFFG010000406 DAZFFG010000000
VERSION     DAZFFG010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.409"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          158 a          134 c           96 g          149 t
ORIGIN      
        1 tacatgccga gatgtaatta tcttgtcatt agagatgaca aaaccggtac ccagtaattt
       61 tacttcgcct gcatcaaccg cgccaacagc gacaatatgt cctgtactaa tcatactcac
      121 tcctttgctc aacagtgcgt tgaccctgct taaaaagtta accaattaac tttctcattt
      181 caagagcaag cgcgcgctcg tatccccgcc acgcctgccc gctttatgta gtggttttca
      241 tgcaggtgca tgacataagc aaaagcccgc cagttctggc gggcctcagc aaaaacgatc
      301 ctaaaacgat catgcggatt catgcagcat agacatgcaa agcttgttag aacggtgatt
      361 cccatgttaa aggaggctta ctcttgttga ggttaaacat ccacagtaac tcctgccaat
      421 acgaacactt tattcatcat agtgttctaa tttcgctttt gctaaacgtc catgtaagtg
      481 attaacaaaa tgtaaattac tcaaaatcag gctcataacg cattttaatt gcatcca
//
LOCUS       DAZFFG010000407          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFFG010000407 DAZFFG010000000
VERSION     DAZFFG010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.410"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..515)
                     /locus_tag="ACHJ7J_005237"
     CDS             complement(<1..515)
                     /locus_tag="ACHJ7J_005237"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5003294.1"
                     /translation="MNKPKVAVLLATYNGECWISAQIDSILAQRDVDVTIYISDDCST
                     DKTKEICSNYSERFNNIKLLSNSERMGGASANFFRLLLSNEYDQHDFVALSDQDDIWK
                     ENKLIHAIKQIGTDGFYSSNVTAFYSDGRQVLINKSQKMTSYDYFFEGGGPGCTYVFS
                     VENAFKLREFLV"
BASE COUNT          175 a          104 c           76 g          181 t
ORIGIN      
        1 actagaaatt ccctaagttt gaatgcattt tcaactgaaa agacgtaagt acaaccgggc
       61 cccccacctt caaaaaaata atcataggag gtcatcttct gggatttatt tattaaaact
      121 tgtcttccat cgctataaaa tgcagttaca ttactggaat aaaagccgtc agttccaatt
      181 tgtttaattg catgaataag tttattttct ttccaaatat catcttgatc agataaagca
      241 acaaaatcat gttgatcata ctcatttgaa agcaataatc ggaaaaaatt agcacttgcg
      301 ccccccatcc gttctgaatt tgaaagtagc ttaatattat taaatctttc tgaatagtta
      361 ctgcaaatct cttttgtttt atcagttgag caatcatcac ttatataaat tgtaacgtcg
      421 acatctcttt gtgcaagaat gctatcgatt tgtgcactga tccaacactc tccattgtat
      481 gtagccagta aaacagcaac tttaggttta ttcatattct catccttgta aggaat
//
LOCUS       DAZFFG010000408          535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFFG010000408 DAZFFG010000000
VERSION     DAZFFG010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.411"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            260..367
                     /locus_tag="ACHJ7J_005238"
     CDS             260..367
                     /locus_tag="ACHJ7J_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5003295.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT          145 a          129 c          147 g          114 t
ORIGIN      
        1 aaaaaccgga atgcactctt cattgcaaac atttcaggga tgaagtgaag atgaattgag
       61 tcgtagtatc cacaccaagc cggcgttatc cggtgaggcg caatgttgcg ggggctttat
      121 ccctggtggc attggttgct ggaaagagaa aacccccgca cgttgagagg taaaacctga
      181 caacagaacg ggggctaatc ttgaaaccag acacttcaag aatagccgca aaatgttgtc
      241 attacaacaa ggcggctata tgacgctcgc gcagtttgcc atgactttct ggcacgacct
      301 ggcggcaccg atcctggcgg gaattattac cgcagcgatt gtcggctggt ggcgtaaccg
      361 gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc
      421 gcaggggtaa cagcctgcgg ccatttttgt ctttatcact tccacaagta aatattggca
      481 acccgagctg gaagcatcat ccagagcact catgaaacga ctgtaacagc tgttg
//
LOCUS       DAZFFG010000409          533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFFG010000409 DAZFFG010000000
VERSION     DAZFFG010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.412"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            3..509
                     /locus_tag="ACHJ7J_005239"
     CDS             3..509
                     /locus_tag="ACHJ7J_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5003296.1"
                     /translation="MTCISQPKKTTLRDKAMVNFAFDYLSSPGSLPFTTAATELSAIH
                     GHSTSQYRLGEFYLHGSDGKPLDYTQARYWSEQSAEQENPRAQSKLGWIYLKGLGVKP
                     DTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAER
                     LLAGLPAH"
BASE COUNT          162 a          126 c          118 g          127 t
ORIGIN      
        1 caattacctg tattagccaa cccaagaaaa cgacgttgcg tgataaagcc atggtgaatt
       61 ttgcctttga ttatttaagc tcaccgggca gtcttccatt caccacggca gccacggagc
      121 tttccgcgat tcatggtcac tcaacgtcgc aatatcgcct tggagaattt tatcttcatg
      181 gtagcgacgg taaaccactg gattatacac aggcgagata ctggtctgag caatcagcgg
      241 aacaggaaaa tccacgcgcg caaagtaaac tggggtggat ctacctcaaa ggtctggggg
      301 tcaaacccga cacccgtaaa gcaattctct ggtataagga agcagctgaa caagggtatg
      361 ctcatgctca atatacttta ggtttgatct acagaaatgg ctcaggtatt aatgttaacc
      421 attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa aatgcggaaa
      481 gattacttgc cgggcttccc gcacattaac gtcgactgtc acccgaacag atg
//
LOCUS       DAZFFG010000410          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFFG010000410 DAZFFG010000000
VERSION     DAZFFG010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.413"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>530
                     /locus_tag="ACHJ7J_005240"
     CDS             <1..>530
                     /locus_tag="ACHJ7J_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5003297.1"
                     /translation="NGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGD
                     LMRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLW
                     ADIVAQGTRHSMKASSDNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLS
                     LDDGQDNAGYVKFGHG"
BASE COUNT          107 a          145 c          173 g          105 t
ORIGIN      
        1 aacggtgaaa ataacagcgt ccgtctcagc attcagggcg gccatctcgg tcacgataac
       61 aacggcggta ttgcccgtgg ggccacgccg gaaagcagcg gcagctatgg cttcgtccgt
      121 ctggagggtg acctgatgag aacagaggtt gccggtatgt ctgtgaccgc gggggtatat
      181 ggtgctgcag gccattcttc cgttgatgtt aaggatgatg acggctcccg tgccggcacg
      241 gtccgggatg atgccggcag cctgggcgga tacctgaatc tgatacacaa cgcctccggc
      301 ctgtgggctg acattgtggc ccagggaacc cgccacagca tgaaagcctc atcggacaat
      361 aacgacttcc gcgtccgggg ctggggctgg ctgggttcgc tggaaaccgg tctgcccttc
      421 agtatcactg acaatctgat gctggagccg caactgcagt acacctggca gggactctcc
      481 ctggatgacg gccaggataa cgccggttat gtgaagttcg ggcatggcag
//
LOCUS       DAZFFG010000411          526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFFG010000411 DAZFFG010000000
VERSION     DAZFFG010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.414"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>526)
                     /locus_tag="ACHJ7J_005241"
     CDS             complement(<1..>526)
                     /locus_tag="ACHJ7J_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5003298.1"
                     /translation="LHFYDYSRRYAINENIAKDAVTNNIALMSTFYDSDNTSGEVLNN
                     DFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSS
                     HYNDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDATNLINLKTLLNDGC
                     FIKYSHFNDVALIK"
BASE COUNT          176 a          103 c           77 g          170 t
ORIGIN      
        1 tgctttatta atgccacgtc attaaaatga ctatatttaa taaagcaacc atcattgagt
       61 agtgttttaa gattgattaa attagttgca tcttcacgta ccatcgtggc aacaggaaca
      121 ggcaatgaca tgaaaacttc ttcattcacc atcaagtgaa tcatgcattg cgttcccttt
      181 gtcatatcca cacttacgct acaaatatcg ttgtaatgcg aagagtgcat ttcaggaaac
      241 agattaaaca cggtatcttg aaaggcacta aatcgtgtta ataaatcctg ataatgaaca
      301 ggttttgttt caaaattatc atcagcaaat ccaagcacta ttgcatgctc tacatctaac
      361 gcagtttcaa atgactcttc ttctgtaaaa tcattattta aaacctcacc tgacgtatta
      421 tcactgtcat aaaaagtgct catcaatgcg atattgtttg ttacagcgtc ttttgcaata
      481 ttctcattaa ttgcatatcg cctgctataa tcataaaaat gaagaa
//
LOCUS       DAZFFG010000412          517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFFG010000412 DAZFFG010000000
VERSION     DAZFFG010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.415"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(19..150)
                     /locus_tag="ACHJ7J_005242"
     CDS             complement(19..150)
                     /locus_tag="ACHJ7J_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003299.1"
                     /translation="MTPGRNQYEAPDSLSNPIVESNDTPANSTQFTLEPFSIFKWDT"
     gene            complement(304..>517)
                     /locus_tag="ACHJ7J_005243"
     CDS             complement(304..>517)
                     /locus_tag="ACHJ7J_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5003300.1"
                     /translation="TAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLA
                     KEKPVEGSPLFLLQMCTEQLVVSGKI"
BASE COUNT          146 a          114 c          107 g          150 t
ORIGIN      
        1 aacttccctg acaagttatt aggtatccca cttgaagatt gagaatggct ccagagtaaa
       61 ctgtgtactg ttcgccggag tgtcattact ctcaacaatc ggatttgaca gtgagtcggg
      121 cgcttcatac tggtttctac ctggagtcat tgtcacttct cccaactgga taaatatatc
      181 gccaattcac aaagtgggcc aaaatcaggt gaaaacgaat tattacgcct ggtaaaggct
      241 atagtacaac taagtgtggc gaatcaggca gtcttgcatt tgtcatcaat ctcatgtctt
      301 acatcaaatt tttccactca ccactagttg ttccgtacac atttgtagta aaaacagtgg
      361 actgccttct accggctttt ctttagccag caacgaaatc tgtcgataga aggttggttc
      421 gagaggaacg gaacacaatt catctgcaat ggttttcagc gttaactctg gtaataaggc
      481 gatgcctaat ccctgtcgaa taaaactgat cgctgta
//
LOCUS       DAZFFG010000413          516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFFG010000413 DAZFFG010000000
VERSION     DAZFFG010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.416"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..399
                     /gene="ybfC"
                     /locus_tag="ACHJ7J_005244"
     CDS             <1..399
                     /gene="ybfC"
                     /locus_tag="ACHJ7J_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO5003301.1"
                     /translation="EDIGEYYWQTELGTCKNNKFTPNGARLLIRVGSLRLNENHAIKG
                     TLQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTY
                     ARMTAHLPNKDESYECLTKIQHELLRSEEK"
BASE COUNT          190 a           87 c          110 g          129 t
ORIGIN      
        1 gaggacatcg gtgagtatta ttggcaaaca gaactgggta catgtaaaaa taataaattc
       61 acaccaaacg gggcgagact cttaattcgc gtcgggtcac tacgccttaa tgaaaatcat
      121 gccatcaaag gaacgttgca ggagttgata aatacagcag aaaaaacggc gtttctcggc
      181 gatcggttta gaagttatat aagagcgggt atctatcaaa aaaagagcag tgatccagct
      241 cagttgttgg cagtccttga taattcaatc atggttaaat attttaaaga tgagaagcca
      301 acttatgctc gaatgacagc acatctcccg aacaaagatg agtcttacga atgtttaaca
      361 aaaatacagc atgaactgct tcgtagtgaa gaaaaataaa acatcttcct gagagaataa
      421 attaaccatt cgggaaaaca tgttcttaat ggatatccag gtacaccaga aggtcgattt
      481 ggaaaaatgg attacgatgg taagaatatc atatat
//
LOCUS       DAZFFG010000414          509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFFG010000414 DAZFFG010000000
VERSION     DAZFFG010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.417"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>509
                     /locus_tag="ACHJ7J_005245"
     CDS             <1..>509
                     /locus_tag="ACHJ7J_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5003302.1"
                     /translation="ATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNR
                     HRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNGMDPLGLYEFKSKNIDDIGIFALA
                     MCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTH
                     GFYSDDKGN"
BASE COUNT          165 a           92 c          131 g          121 t
ORIGIN      
        1 gctacagaat ggtgcgcaga atacgatgag tggggcaacc tgctgaatga agagaacccg
       61 catcagctgc agcagctcat ccgcctgccg gggcagcagt atgatgagga gtccggcctg
      121 tattacaacc gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatccg
      181 attgggctga aagggggatg gaacctgtat acatatccgc tgagcccggt gaatggcatg
      241 gatccattag gattatatga atttaaatca aaaaatatag atgatattgg aatatttgca
      301 ttggcaatgt gtaatggaga atcaattaac gagaataaag aatatggtgg actaatatgt
      361 aagaagcaag gtgaatattt ccccatgaat ccgataagtt caaatgataa tgatagtgta
      421 gacttgcgaa atataaaatg ccctgaaggt tcagagagag taggcgatta tcacactcac
      481 ggtttttact ctgacgataa aggaaataa
//
LOCUS       DAZFFG010000415          503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFFG010000415 DAZFFG010000000
VERSION     DAZFFG010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.418"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>503
                     /locus_tag="ACHJ7J_005246"
     CDS             <1..>503
                     /locus_tag="ACHJ7J_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5003303.1"
                     /translation="LSREEQAVLGIKYRQLKESFNDDDNVKHINSDLDITNDISDKNM
                     KIVADISGNTNLEANLQLSLDGITLPFIGKGEQHKVQIKLAMQNKANDVNVILIEEPE
                     NHLSHMNLSSLVNFIEKKHEGKQLFISTHSSFVMNKLSLNKICLLGNGYKKLDKIDSG
                     TVKNLKR"
BASE COUNT          207 a           72 c           77 g          147 t
ORIGIN      
        1 tacttagtag agaagaacaa gctgtactgg gtataaaata tcgacaactg aaagaaagtt
       61 tcaatgatga tgataatgta aaacatatta acagcgacct tgatataaca aatgacattt
      121 cagataagaa catgaaaatt gttgctgata tatcaggaaa cactaatttg gaagcaaacc
      181 ttcaattatc acttgatggt ataactttac cctttattgg aaagggtgaa caacataaag
      241 tacaaattaa gcttgcaatg caaaataaag ctaatgatgt taatgttatt ttgattgagg
      301 aaccagaaaa ccacctctct catatgaatc tatcatcatt agttaacttt attgaaaaaa
      361 aacatgaagg taaacaacta tttattagta cgcacagttc atttgttatg aacaaattaa
      421 gcctcaacaa aatttgtcta ctcggtaatg gttataaaaa acttgataaa atagactctg
      481 gtacagttaa aaaccttaaa aga
//
LOCUS       DAZFFG010000416          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFFG010000416 DAZFFG010000000
VERSION     DAZFFG010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.419"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(19..375)
                     /locus_tag="ACHJ7J_005247"
     CDS             complement(19..375)
                     /locus_tag="ACHJ7J_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5003304.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT          175 a           98 c          103 g          123 t
ORIGIN      
        1 atcgccccaa ttctaagatt aatttgaacc aaacttcgga tcgtttccgt aacgattgct
       61 gccagaagta ccttctgtgc agaaaaaaac cagaagcacg agccagccaa taaacggaac
      121 aaaaaagagc aaggcccagg cacctgaacg gtcggtatcg tgtaagcgac ggatagcaag
      181 cgcaaggacg ggaaggaagg ttgccagcaa ataaagcata gacagatatg gaagctccag
      241 acctaaaatc aattgaatga cattgataat agcgccgacg atggcgttaa tcagagtaaa
      301 catccagtac tctttacgac gtgcgcgacc agagaaacca acataatttt ttaatacgga
      361 taaataccac tgcataaaaa catcccttta attaaaagtg acgaaagtct attccgggta
      421 ttgtgtaaat gctattcgta tctttctttt tcggatgtta taaaggtttt gatatttaag
      481 ataaatacat ggttatttt
//
LOCUS       DAZFFG010000417          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFFG010000417 DAZFFG010000000
VERSION     DAZFFG010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.420"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..356
                     /locus_tag="ACHJ7J_005248"
     CDS             <1..356
                     /locus_tag="ACHJ7J_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5003305.1"
                     /translation="PCYVRSNIEGHNITAHIWVYPSLTEVECEVLELLVKGYSVTRIS
                     ESRFRSVKTVSLQKHQIYKKLGIRNDITFWLDLSLSSCVKMKFTYKEGTVKESPLSCL
                     NNVSNIAHNISCKNP"
BASE COUNT          161 a           80 c          103 g          154 t
ORIGIN      
        1 ttccctgtta tgtaagaagt aatattgaag ggcataatat cactgctcat atatgggtgt
       61 atccgtcact gacagaggtt gaatgtgagg tgcttgaatt attagtgaaa ggctattcgg
      121 ttacccggat ctcagagagc aggtttcgca gcgttaaaac agtttcatta cagaaacatc
      181 agatttataa gaagctgggg atccgtaatg acatcacatt ctggctggat ctttcattat
      241 cttcctgtgt aaaaatgaaa tttacctata aagagggaac agttaaagaa tcaccactat
      301 catgtctgaa taatgtatca aatatcgcgc ataacatttc atgtaagaat ccttaacagg
      361 agtgaggttg cttccgtgat aaaatcagat aaatatttct tactggagta gaggtcagaa
      421 aaatttcaca atatatcaaa taaatagggc gttctcttgc tttctgtgat ggccttttat
      481 tcggaaagga agtatcat
//
LOCUS       DAZFFG010000418          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFFG010000418 DAZFFG010000000
VERSION     DAZFFG010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.421"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..225
                     /locus_tag="ACHJ7J_005249"
     CDS             <1..225
                     /locus_tag="ACHJ7J_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFO5003306.1"
                     /translation="VNYSIYRKAVEDLATRGFSIVNSQGSESRNPALSAKADAERIMI
                     KMASLLGFDPVSRRRNPPETEEEDELDRLA"
     gene            209..>492
                     /locus_tag="ACHJ7J_005250"
     CDS             209..>492
                     /locus_tag="ACHJ7J_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5003307.1"
                     /translation="MTAWHEYAEGVKNGKITACKRLKQAVKRYFSDLENSLYTFDPEV
                     VERFIAFSRVCPHVKGAMRGSPIELEPWQQFAFACILGFKVKATGRRKYT"
BASE COUNT          132 a          125 c          135 g          100 t
ORIGIN      
        1 gttaactact ccatataccg caaagccgtc gaagaccttg cgacgcgcgg gttcagcatt
       61 gttaacagtc agggcagcga gagcagaaac cccgcactga gcgcaaaggc tgacgcagaa
      121 agaataatga tcaaaatggc ttctttgctg ggttttgacc cggtaagccg tcgcagaaat
      181 ccaccggaaa cagaggaaga ggacgaactt gaccgcctgg catgagtacg cagaaggcgt
      241 aaaaaacggc aaaattacgg cctgtaaacg actgaaacag gccgttaaac ggtatttttc
      301 tgaccttgaa aactcccttt acacgttcga tccggaggtc gtggagcggt ttattgcctt
      361 ttcccgggtg tgtccgcacg taaaaggcgc aatgcgcggt agtcccattg aactggagcc
      421 gtggcagcag ttcgcctttg cctgcatcct gggctttaag gttaaggcca ccggacggcg
      481 caaatacacc ag
//
LOCUS       DAZFFG010000419          487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFFG010000419 DAZFFG010000000
VERSION     DAZFFG010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.422"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(39..>487)
                     /locus_tag="ACHJ7J_005251"
     CDS             complement(39..>487)
                     /locus_tag="ACHJ7J_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pertussis toxin-like subunit ArtA"
                     /protein_id="HFO5003308.1"
                     /translation="IRTNNVFYSLAPSVQLLRERGVTFSRFEEIMMREQSEIVADGRI
                     PTENIAEAVQLNYERTTGIVSDGHGTTNSRFLNIPTDSNPGIIPNIPVPQVSVRQRIN
                     AFGSLITACFSLRGSRDEENGENVDVVTFYDARPVLKTLLVSTSIR"
BASE COUNT          154 a          104 c           71 g          158 t
ORIGIN      
        1 atgataattt atcacaaaac attatttatt aaataatgtc acctgattga tgtactaacc
       61 aataatgttt tcaggacagg ccgtgcgtca taaaatgtaa caacatcaac attttctcca
      121 ttttcttcat cacgacttcc tctcaaagaa aaacatgcag taattaacga gccaaaagca
      181 ttaattctct gacgaacact cacctgaggt actggaatat ttggaataat acctggattt
      241 gaatccgtcg gtatattaag aaaccttgag tttgttgttc catgaccatc gctaactatt
      301 ccggtagttc tttcatagtt taactgtact gcttccgcta tattttctgt cggtattcga
      361 ccatcagcaa caatttcact ttgctcacgc atcataatct cttcgaatcg actaaaagtt
      421 acacctctct cccttaagag ttgaacagat ggagcaagtg aataaaaaac attattggtt
      481 cttatcc
//
LOCUS       DAZFFG010000420          482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFFG010000420 DAZFFG010000000
VERSION     DAZFFG010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.423"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            2..>482
                     /locus_tag="ACHJ7J_005252"
     CDS             2..>482
                     /locus_tag="ACHJ7J_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5003309.1"
                     /translation="MIMDNENKAPLFYPNLYLTIQLRNRECSVLTMEATALTLSLFYR
                     FLRENDISIDERILHNRFISNSEIERLFIFLTKKRSRRIRSQSISKLTLYHRLNTIAG
                     YLSWLTETLYRYDTPEINILNSEKMVRSIKERKPIIPKRQDRTYENDGIAENVIKIIM
                     "
BASE COUNT          181 a           70 c           81 g          150 t
ORIGIN      
        1 catcattatg gataacgaaa ataaagcccc gcttttttat cctaatttat acttaacaat
       61 tcagttaagg aatagggaat gttctgtgtt aacaatggaa gcgacagcct taacactgtc
      121 gctattttat agatttttac gagagaatga tatatcaatt gatgaacgta ttttacataa
      181 tagattcata tcgaattctg aaattgagcg cttgttcatt ttcttaacaa aaaaacgttc
      241 aaggcgcata cgttcacaaa gtatatcaaa gttaacatta tatcacaggt taaataccat
      301 cgctggttat ttatcgtggt taacagaaac gctgtataga tatgatacac cagaaattaa
      361 tatattaaat tcagagaaaa tggttcgttc tatcaaagaa aggaagccaa taatacctaa
      421 gaggcaagat aggacttatg aaaatgacgg tattgctgaa aatgtcataa aaattattat
      481 gg
//
LOCUS       DAZFFG010000421          475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFFG010000421 DAZFFG010000000
VERSION     DAZFFG010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.424"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(100..>475)
                     /locus_tag="ACHJ7J_005253"
     CDS             complement(100..>475)
                     /locus_tag="ACHJ7J_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO5003310.1"
                     /translation="SLTTGNVSELHSSSEPSRQGVFPVICTQLHPGTVTKRQLLTGWG
                     PVFIIGDDPFSLRWMSEHLEKFKSLNALGLVVNVASADRMELLNKQADGLLLVPVVCD
                     NLMQLLQLNAYPVLITETEISQ"
BASE COUNT          158 a          100 c           83 g          134 t
ORIGIN      
        1 taatagttat catgaaaatt cataaacacc atctattcta tgagatgatt atcagtaatt
       61 atggaaacat cagcaacatg cttatgagat ggtatacatt cactgagata tttccgtttc
      121 tgtgattaat accggataag catttaattg caaaagctgc atcagattat cgcagacaac
      181 aggcaccaat aataatccat cagcctgctt atttaatagt tccattcggt ctgctgatgc
      241 aacattcaca accaaaccga gggcatttag cgatttaaac ttctccaggt gttcactcat
      301 ccatcgcaga gaaaaaggat catcgccaat tataaaaaca ggtccccagc cagttaataa
      361 ctgacgtttc gttacagtac cgggatgaag ttgagtacat attacaggaa ataccccctg
      421 tcgtgatggc tccgatgacg aatgtaattc agaaacattt cctgttgtca acgaa
//
LOCUS       DAZFFG010000422          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFFG010000422 DAZFFG010000000
VERSION     DAZFFG010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.425"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>472
                     /locus_tag="ACHJ7J_005254"
     rRNA            <1..>472
                     /locus_tag="ACHJ7J_005254"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          118 a          113 c          142 g           99 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg ga
//
LOCUS       DAZFFG010000423          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFFG010000423 DAZFFG010000000
VERSION     DAZFFG010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.426"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..133
                     /locus_tag="ACHJ7J_005255"
     CDS             <1..133
                     /locus_tag="ACHJ7J_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO5003311.1"
                     /translation="LETAKRNGLEPHAWLTDVLMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(268..>469)
                     /locus_tag="ACHJ7J_005256"
     CDS             complement(268..>469)
                     /locus_tag="ACHJ7J_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003312.1"
                     /translation="AFFRRASSAAHTNDRGLITVDLFCCTGAIARQVPEAGSEVLSRV
                     IASGREVLTRGLMAGDDVLTRE"
BASE COUNT          107 a          146 c          129 g           87 t
ORIGIN      
        1 gctggaaacc gcgaaacgca acggtctgga gccgcatgcc tggctgacag acgtcctgat
       61 gcgtctgccg gagtggccgg aggagcgact ggcagagttg ctgcctcttg agggatttac
      121 cttctccggg tgagtgatac ctgccgtcag gtgttcgtgc accgggccat aacctgcagt
      181 cgggagttga actcctgacg acaggaaatg agccagacca gcaaccacac gggctgacag
      241 cgaataagaa cctgaatcag cccggagtca ttcccgggtc agtacatcat cacctgccat
      301 taatccccga gtcagtactt cgcggcctga cgcgatcacc ctactcagta cttcagagcc
      361 cgcctccggt acctgcctgg cgatcgcccc tgtgcaacaa aataaatcaa ccgttatcag
      421 accacgatcg ttcgtatgcg ccgccgatga cgcgcgcctg aaaaacgcg
//
LOCUS       DAZFFG010000424          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFFG010000424 DAZFFG010000000
VERSION     DAZFFG010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.427"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..104)
                     /gene="yrhD"
                     /locus_tag="ACHJ7J_005257"
     CDS             complement(<1..104)
                     /gene="yrhD"
                     /locus_tag="ACHJ7J_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFO5003313.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFS"
     gene            complement(199..>468)
                     /gene="yrhA"
                     /locus_tag="ACHJ7J_005258"
     CDS             complement(199..>468)
                     /gene="yrhA"
                     /locus_tag="ACHJ7J_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HFO5003314.1"
                     /translation="NGLRLFSLSIPEPSVKNLFAVNEFYRNNDDFINPDLQERLVIGD
                     DSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIMDSCS"
BASE COUNT          151 a           76 c           74 g          167 t
ORIGIN      
        1 tcactaaaat catcatagcc attagctatt tcggctaaaa tagagactac atgtcttcgg
       61 tccatctcac ttaaggagtg tagttccgtt gtaagttttt ccatagcttg cattgctaaa
      121 tttcgaacaa ggaattttct ggtggtaatc tctaaaaaga tggcatggtt tacaatgatt
      181 tttgtttcct tttgattatt atgaacaact gtccatgatt tcgtttaaga atgaagagaa
      241 atcactaaac gaactgaata tattttctgt gccaatatta tctctaattt caaaaaagtt
      301 acttttaatg tcataagtaa atattgaaat gctatcatcc ccgatcacta accgttcttg
      361 tagatcaggg tttatgaaat catcattatt tctataaaat tcatttacgg caaaaaggtt
      421 tttaactgaa ggttctggaa tcgagaggct aaataatcgt aatccatt
//
LOCUS       DAZFFG010000425          458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFFG010000425 DAZFFG010000000
VERSION     DAZFFG010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.428"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>458)
                     /locus_tag="ACHJ7J_005259"
     rRNA            complement(<1..>458)
                     /locus_tag="ACHJ7J_005259"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           83 a          146 c          101 g          128 t
ORIGIN      
        1 gcgtgccttc tcccgaagtt acggcaccat tttgcctagt tccttcaccc gagttctctc
       61 aagcgccttg gtattctcta cctgaccacc tgtgtcggtt tggggtacga tttgatgtta
      121 cctgatgctt agaggctttt cctggaagca gggcatttgt tgcttcagca ccgtagtgcc
      181 tcgtcatcac gcctcagcct tgattttccg gatttgcctg gaaaaccagc ctacacgctt
      241 aaaccgggac aaccgtcgcc cggccaacat agccttctcc gtcccccctt cgcagtaaca
      301 ccaagtacag gaatattaac ctgtttccca tcgactacgc ctttcggcct cgccttaggg
      361 gtcgactcac cctgccccga ttaacgttgg acaggaaccc ttggtcttcc ggcgagcggg
      421 cttttcaccc gctttatcgt tacttatgtc agcattcg
//
LOCUS       DAZFFG010000426          451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFFG010000426 DAZFFG010000000
VERSION     DAZFFG010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.429"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..365
                     /locus_tag="ACHJ7J_005260"
     CDS             <1..365
                     /locus_tag="ACHJ7J_005260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003315.1"
                     /translation="APLPENSIVFDISAISTGGADAVLVWANDEYVDDNGYTVAKDAN
                     GKKWPLLFSINDFTCSNGVVSYKNVNNQVLDVGYNDEVTSCEGLNGTATLNVHARNSQ
                     EWPAGSYTLKLFAVGWID"
BASE COUNT          139 a           74 c           97 g          141 t
ORIGIN      
        1 cagccccact tccagaaaac agtatcgtat ttgatatatc ggctatttca actggaggtg
       61 ctgatgctgt tttagtatgg gctaatgatg agtatgttga tgataacggc tacactgtag
      121 caaaagatgc aaatggtaag aaatggcctt tattattttc tattaacgac tttacatgct
      181 ccaatggagt tgtttcatat aaaaatgtaa ataatcaagt gttagatgta ggatataatg
      241 atgaagtgac atcctgtgag ggattgaatg gtactgccac actgaatgta catgcacgga
      301 atagccaaga atggcctgca ggttcatata cattaaaact attcgctgtt ggctggatag
      361 actgatcaga gttcatgttc tctgtgtatg atgctctatc acaatcatag aggtaattaa
      421 ccatatacag gtgtcctgtc tgtaaatact c
//
LOCUS       DAZFFG010000427          451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFFG010000427 DAZFFG010000000
VERSION     DAZFFG010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.430"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>451
                     /locus_tag="ACHJ7J_005261"
     CDS             <1..>451
                     /locus_tag="ACHJ7J_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO5003316.1"
                     /translation="TKTALSGVQAGQAATMASATGDPNAMGVSLSLTTQKSKSQQHSE
                     SDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKAGGNTSLDAANDILLSGAA
                     NTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFAGVNAAKGSEKGNGT"
BASE COUNT          121 a          111 c          140 g           79 t
ORIGIN      
        1 ccacaaagac agcgctgtct ggtgtgcagg ccggacaggc tgcgacaatg gcctccgcaa
       61 ccggtgaccc gaatgcgatg ggagtcagcc tgtcgctcac cacccagaaa tcgaaatcac
      121 aacaacattc tgaaagtgac acagtatccg gcagtacgct gaatgccggg aataatctgt
      181 ctgttgtcgc aaccggtaaa aacaggggcg ataaccgcgg agatattgtg attgccggaa
      241 gccagcttaa ggccggtggt aacacaagcc tggatgccgc gaatgatatt ctgttgagtg
      301 gcgccgcaaa cacacaaaaa acaacgggca ggaacagcag cagtggcggt ggcgtgggtg
      361 tcagtatcgg tgccggtggt aacggtgccg gtatcagcgt ctttgccggc gttaatgcgg
      421 caaaaggcag cgagaaaggt aacggtactg a
//
LOCUS       DAZFFG010000428          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFFG010000428 DAZFFG010000000
VERSION     DAZFFG010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.431"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>448
                     /locus_tag="ACHJ7J_005262"
     CDS             <1..>448
                     /locus_tag="ACHJ7J_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO5003317.1"
                     /translation="TKTALSGVQAGQAAAMATATGDPNATGVSLSLTTQKSKSQQHSE
                     SDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKAGGNTSLDAANDILLSGAA
                     NTQKTTGRNSSSGGGVGVSIGAGKGAGISAFASVNAAKGREKGNGI"
BASE COUNT          125 a          109 c          139 g           75 t
ORIGIN      
        1 ccacaaagac agcgctgtct ggtgtgcagg ccggacaggc tgcggcaatg gccaccgcaa
       61 ctggtgaccc gaatgcgacg ggagtcagcc tgtcgctcac cacccagaaa tcgaaatcac
      121 aacaacattc tgaaagtgac acagtatccg gcagtacgct gaatgccggg aataatctgt
      181 ctgttgtcgc aaccggcaaa aacaggggcg ataaccgcgg agatattgtg attgcaggaa
      241 gccagcttaa ggccggtggt aacacaagcc tggatgccgc gaatgatatt ctgttgagtg
      301 gcgccgcaaa cacacaaaaa acaacgggca ggaacagcag cagtggcggt ggcgtgggtg
      361 tcagtatcgg tgcaggtaaa ggtgccggta tcagcgcctt tgccagcgtt aatgcggcaa
      421 aaggcaggga gaaaggtaac ggtatcga
//
LOCUS       DAZFFG010000429          444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFFG010000429 DAZFFG010000000
VERSION     DAZFFG010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.432"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>444
                     /locus_tag="ACHJ7J_005263"
     CDS             <1..>444
                     /locus_tag="ACHJ7J_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5003318.1"
                     /translation="QRPEKDQIITYQKSLGNETKGMILTNCSCVKTTFNWADLSESDC
                     QKVDFSEANLSNTILPDIVMMKDTKLYRTDLFNPILKAEAESTEEKDISPLAKIILDY
                     IESDKNPESLKFDEKSTVIKIKQDIDNFIFYNQHLKKIFNRAMNLL"
BASE COUNT          180 a           78 c           64 g          122 t
ORIGIN      
        1 caacgtccag aaaaagatca aataataact tatcagaaat cacttggaaa tgaaacgaaa
       61 gggatgattc taactaactg ctcctgtgtt aaaaccactt tcaattgggc agatttatca
      121 gaaagtgatt gccaaaaggt ggactttagc gaggctaatc tttcaaacac tattcttcct
      181 gacatcgtca tgatgaaaga cacgaaactt taccgtaccg acctttttaa tccgatatta
      241 aaagcagaag cagaatcaac agaagaaaaa gacattagcc cattagcaaa aattattctt
      301 gactatattg aatcagataa aaaccctgag tcattaaaat ttgatgaaaa atcaacagtt
      361 attaaaataa aacaagatat cgataatttt attttctaca atcagcattt aaagaaaata
      421 ttcaatcgcg caatgaatct tcta
//
LOCUS       DAZFFG010000430          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFFG010000430 DAZFFG010000000
VERSION     DAZFFG010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.433"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>443
                     /locus_tag="ACHJ7J_005264"
     CDS             <1..>443
                     /locus_tag="ACHJ7J_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO5003319.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVV"
BASE COUNT          113 a          103 c          122 g          105 t
ORIGIN      
        1 ccgatcgaag acgtattctc catctccggt cgtggtaccg ttgttaccgg tcgtgtagaa
       61 cgcggtatca tcaaagttgg tgaagaagtt gaaatcgttg gtatcaaaga gactcagaag
      121 tctacctgta ctggcgttga aatgttccgc aaactgctgg acgaaggccg tgctggtgag
      181 aacgtaggtg ttctgctgcg tggtatcaaa cgtgaagaaa tcgaacgtgg tcaggtactg
      241 gctaagccgg gcaccatcaa gccgcacacc aagttcgaat ctgaagtgta cattctgtcc
      301 aaagatgaag gcggccgtca tactccgttc ttcaaaggct accgtccgca gttctacttc
      361 cgtactactg acgtgactgg taccatcgaa ctgccggaag gcgtagagat ggtaatgccg
      421 ggcgacaaca tcaaaatggt tgt
//
LOCUS       DAZFFG010000431          440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFFG010000431 DAZFFG010000000
VERSION     DAZFFG010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.434"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..372
                     /locus_tag="ACHJ7J_005265"
     CDS             <1..372
                     /locus_tag="ACHJ7J_005265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003320.1"
                     /translation="TNKLENPDDFFSGTLSMDIQSDSVGIIRVFGSVTTKSSTSGVNH
                     YRVNRDITFDVMDFKKNTFYISEVKTIAHPGDNVPAFEFAGYVVNVINFDSDRIVVSK
                     HNNTFIFGGETLPSFICVKNN"
BASE COUNT          131 a           71 c           89 g          149 t
ORIGIN      
        1 actaataagc tggaaaaccc tgatgatttc ttttctggaa cattatcaat ggatattcaa
       61 tcagatagtg taggtataat cagggttttt ggttctgtta cgaccaaatc atcgacctct
      121 ggtgtcaacc actatcgcgt taaccgtgat ataacctttg atgtgatgga tttcaaaaag
      181 aatacttttt atattagtga agtgaaaact attgctcatc caggtgataa cgttcctgcg
      241 tttgaatttg cagggtatgt agtcaatgtt attaattttg atagcgacag gatcgtggtc
      301 agtaaacata ataacacttt catttttggt ggcgaaacac ttccttcatt tatttgtgtc
      361 aaaaacaact aaccttagaa gaaagggcag gtatcctgct atctttgagt gattttgtag
      421 gagccagcta gtttgaagat
//
LOCUS       DAZFFG010000432          435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFFG010000432 DAZFFG010000000
VERSION     DAZFFG010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.435"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(10..>435)
                     /locus_tag="ACHJ7J_005266"
     CDS             complement(10..>435)
                     /locus_tag="ACHJ7J_005266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003321.1"
                     /translation="APSKHQEAVRNGGSEMRKFKIIIETGIAGGDFEDVFEVDDDATP
                     DEIHDEAKEIFFNYCNYSYHGIKDEEEVLFKSYAHEVTTTFEVVFFLKTDDEIFGLNE
                     RGQITPTQITEHLSIDELFYFIDLPKPPSFSNAMFPEYL"
BASE COUNT          125 a           87 c           70 g          153 t
ORIGIN      
        1 attgaaaatt tataaatatt caggaaacat agcattactg aaagatggtg gttttggtaa
       61 atcaataaag taaaaaagct catctatgct taaatgctca gtaatttgcg tgggcgttat
      121 ttgtcctctt tcattcaaac caaatatttc atcgtcagtt ttcagaaaaa acaccacttc
      181 aaatgttgtg gttacttcat gcgcataaga tttgaacagc acttcctctt cgtcttttat
      241 tccgtgatat gagtaattgc agtagttaaa gaaaatttct tttgcttcgt catgaatttc
      301 atcaggtgtt gcgtcatcgt ccacttcgaa tacatcctca aaatctccac cagctattcc
      361 cgtttcaata attattttga actttcgcat ttcactaccg ccatttcgaa cggcctcctg
      421 atgttttgag ggtgc
//
LOCUS       DAZFFG010000433          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFFG010000433 DAZFFG010000000
VERSION     DAZFFG010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.436"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          137 a           82 c           71 g          141 t
ORIGIN      
        1 gagtataaaa tctcttctga tgtttaattg atttgaatgt tcgtaagcta tatcacttac
       61 tcaatccatt ttacccagag tcataaaata gagtgtctgt tgatctcatt ttcatattcc
      121 tttcagactg aagtgcgttt tcatcacccg atgagaaaga actgaagcga tgtttgccat
      181 agattgcaag atgccctgtc tcattgtcta tcgtggtgca cctttatacc tgttatacca
      241 gatcaaaaat cacgcaatcc atacaacaaa accagatttg caattcgtgt cacaaaatat
      301 gtcgatcttt ttctaagaag aagatgccat gtgaagccag acgaacactt gcggtggtct
      361 tcaaaaacta aagatcttct tttaactatg tgttttataa ataatttatt taaagtctaa
      421 acaagggaag c
//
LOCUS       DAZFFG010000434          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFFG010000434 DAZFFG010000000
VERSION     DAZFFG010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.437"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>428)
                     /locus_tag="ACHJ7J_005267"
     CDS             complement(<1..>428)
                     /locus_tag="ACHJ7J_005267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003322.1"
                     /translation="CAAAVALPAASVAFVDAVVALSFASDACLVASSFEADADDAIDA
                     AEAADNAAAVFDEAALFSDAFVFVSDVFAAEADFAASLACSAASPAFVVAVEADDAAL
                     SADFPAAVALAEAFVSDVFAAPALVAASRFEDSAAAALFDA"
BASE COUNT          126 a          125 c          128 g           49 t
ORIGIN      
        1 gcatcaaaaa gtgctgccgc agcagagtcc tcaaaacgcg aggcggccac cagtgccggt
       61 gcggcgaaaa cgtcagaaac gaatgcctca gccagtgcca ccgccgccgg aaaatcggca
      121 gaaagtgctg catcgtccgc ttcaacagcc acaacgaagg ctggcgaagc cgctgaacag
      181 gccagcgaag cagcgaagtc tgcttccgca gcgaagacat ctgaaacaaa cacgaaagcg
      241 tcagaaaaca gggcggcttc gtcaaaaacg gcagcagcat tatcagcagc ttcggcagcg
      301 tcaatggcat catctgcgtc tgcttcaaaa gatgaggcga ccagacaggc gtcagaagcg
      361 aaggacagtg ccacgacggc atccacgaag gcgacagagg cagcgggcag tgcgacggcg
      421 gcagcaca
//
LOCUS       DAZFFG010000435          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFFG010000435 DAZFFG010000000
VERSION     DAZFFG010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.438"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..75)
                     /locus_tag="ACHJ7J_005268"
     CDS             complement(<1..75)
                     /locus_tag="ACHJ7J_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003323.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKK"
     gene            93..284
                     /gene="yfgG"
                     /locus_tag="ACHJ7J_005269"
     CDS             93..284
                     /gene="yfgG"
                     /locus_tag="ACHJ7J_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO5003324.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          101 a          109 c           84 g          134 t
ORIGIN      
        1 ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc
       61 cacagcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt atgcgaaaac
      121 gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt atcttcttct
      181 ttggccgttt tatctactcg tccgtcggtg cctggcagca ccatcagagc aaaaaagaag
      241 ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta ccttctccac
      301 tttcacagaa cataacggca cttcgctgtc ggatgctttt gctctttggg attatcaaag
      361 cggcagatat tctttcatct taaattttac gtctttgtcc tgactgatgt ttatcctgtt
      421 tggctgcg
//
LOCUS       DAZFFG010000436          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFFG010000436 DAZFFG010000000
VERSION     DAZFFG010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.439"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>427
                     /locus_tag="ACHJ7J_005270"
     CDS             <1..>427
                     /locus_tag="ACHJ7J_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFO5003325.1"
                     /translation="FKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTN
                     FWDKNRQLTTDEISCYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPS
                     WPKEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFN"
BASE COUNT          164 a           90 c           61 g          112 t
ORIGIN      
        1 gttcaaaaaa ctattctctc cttcgaaatt aatgacaatt atcggtgagg acattcctct
       61 catatcagaa aaacagtcac tttcaaaggt acttttaaat gataagaata atgaactgag
      121 cgatgggaca aacttctggg ataaaaatcg tcaattaacc acagatgaaa tatcttgcta
      181 tcttaaaaag atcgccgcca atgcaaaaaa tactcaagtc aattatccta ctgatttcta
      241 cctccccaat tccaacagca cttacctgga agtcgctctc aatgataata ttaagagcga
      301 tccatcatgg ccgaaagaag tccagttatt ccccataaat actggcggac actggatatt
      361 agtttcgcta cagaaaatag ttaatgaaaa aaacaacaca caacaaataa aatgcatcat
      421 attcaat
//
LOCUS       DAZFFG010000437          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFFG010000437 DAZFFG010000000
VERSION     DAZFFG010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.440"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..120
                     /locus_tag="ACHJ7J_005271"
     CDS             <1..120
                     /locus_tag="ACHJ7J_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003326.1"
                     /translation="GFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          129 a           90 c           66 g          140 t
ORIGIN      
        1 ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc cctgaaatat
       61 ctcagcgatc acccgaaact gcagggtatt gcccagcaga acagctttaa acacacctga
      121 taacataacg ttgtaaaaac cgaatgccca gcctttaaaa aaacagctgg gcattcggtt
      181 gcttattaat cgcaataata tattggctgt ctattcatcg tgttaataag atatatgcta
      241 tatgaatgtt tattaaatgt agcgattacc tttaatgtca cgaaaagata ttatttgctt
      301 actattaatt tccctgcgtt cttaaacacg ttttttgtat tataaatcca gtcatcctgc
      361 ccgtagaaca tcacacatta tcatcctgtt ctcccgcttc gtttaaattt tttatcaatc
      421 aattt
//
LOCUS       DAZFFG010000438          416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFFG010000438 DAZFFG010000000
VERSION     DAZFFG010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.441"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..377)
                     /locus_tag="ACHJ7J_005272"
     CDS             complement(<1..377)
                     /locus_tag="ACHJ7J_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HFO5003327.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISYNRFTYYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYT"
BASE COUNT           91 a          121 c          107 g           97 t
ORIGIN      
        1 gtatagccct gcagcagtga acggttaccg ccggtcactt cgttgatgat acccttcgct
       61 tccccacccg ctttcaggtt cgggttattc tgtatcagtc caccaagctg cgtcggatta
      121 agcttaccgg tggcattatt gagaatcagc ccttccttcc cgacgttgta atacgtaaac
      181 cggttatacg aaatcccggc cccgttcggc gtggcaatgt tcacgaccgg cacaccattt
      241 gccgctttat ccattccggc cccgttttgt ggggtgatga cggcccccac agccggtaac
      301 aacggctgcc cggcgataat cgtactgata aggtaactca gcaggcggta agtgaagcga
      361 acgggaggct gatgcatggt aataatccct taaaacgcga cggcaacgcg ccagta
//
LOCUS       DAZFFG010000439          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFFG010000439 DAZFFG010000000
VERSION     DAZFFG010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.442"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>414
                     /locus_tag="ACHJ7J_005273"
     CDS             <1..>414
                     /locus_tag="ACHJ7J_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO5003328.1"
                     /translation="AFSTMHSLVIGQIKTDEKSNEIAAIPELLNMLDIKGKIITTDAM
                     GCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGR
                     EEIRLHNVCDVPDELIDFTFEWKGLKKLCVAVSFRS"
BASE COUNT          140 a           71 c           98 g          105 t
ORIGIN      
        1 tgcgttctca acaatgcaca gtctggtcat cggacagatc aagacggatg agaaatctaa
       61 tgagattgca gctatcccag aacttcttaa catgctggat attaaaggaa aaattatcac
      121 aactgatgct atgggttgcc agaaagatat cgcagagaag atacaaaaac agggcggtga
      181 ttatttattc gctgtaaaag gaaatcaggg gcggcttaat aaagcctttg aggaaaaatt
      241 tccactgaaa gaattaaata atccagagca tgacagttac gcaatgagtg aaaagagtca
      301 cggcagagaa gagatccgtc ttcataatgt ttgcgatgtc cctgatgaac ttattgattt
      361 cacgtttgaa tggaaagggc tgaagaaatt atgcgtggca gtctcctttc ggtc
//
LOCUS       DAZFFG010000440          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFFG010000440 DAZFFG010000000
VERSION     DAZFFG010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.443"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>413
                     /locus_tag="ACHJ7J_005274"
     CDS             <1..>413
                     /locus_tag="ACHJ7J_005274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003329.1"
                     /translation="AHSFISNETVDINNSSLTLYPNIKVDKENLSTYTAKKWNLTGDR
                     STFRVGNQTSLNGDISVEKYGDIYIGEESSISGYSSTWSGNLRAPYANISVNNTHWNI
                     IGNSSAEQMYLNKSQLDFDKSNNFSTLTANDFHSR"
BASE COUNT          163 a           59 c           62 g          129 t
ORIGIN      
        1 gcacattctt ttatatctaa tgaaactgta gatatcaata actcatcttt aacgctatat
       61 ccaaatatta aagttgataa agaaaattta tcaacttata cagccaaaaa atggaatcta
      121 acaggagata ggtcaacatt tagagtagga aatcaaactt ccctaaatgg agatatttct
      181 gtagaaaaat atggagatat ttatataggt gaggaaagtt ctatatctgg atatagttct
      241 acatggtcag gaaacttgag agcaccatat gctaatattt cagtaaacaa tacgcactgg
      301 aatattattg gaaactcaag tgcagaacaa atgtatctta ataaatcaca acttgatttt
      361 gataaaagta ataatttttc aacattaact gctaatgatt ttcatagccg tca
//
LOCUS       DAZFFG010000441          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFFG010000441 DAZFFG010000000
VERSION     DAZFFG010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.444"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>412
                     /locus_tag="ACHJ7J_005275"
     CDS             <1..>412
                     /locus_tag="ACHJ7J_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003330.1"
                     /translation="PIKLLASYKDKELARTIINDNIVEKTHDDINNAEFTDGGIDDRS
                     DIGIDSNDTDDIDIDNQEPIKSPANDENKKVGQTIINENIVENTHDINNKKMIFSAIK
                     EIYDEDPSFIFDKISHKLRHTVTEFDESGKSEPTG"
BASE COUNT          171 a           71 c           74 g           96 t
ORIGIN      
        1 ccaataaaat tacttgcgag ctataaagac aaagaactcg cccgtactat cattaatgac
       61 aacatagttg agaagactca tgatgatata aataatgctg aatttactga tggtggtatt
      121 gatgatcgta gtgatattgg cattgactcc aatgatacag acgatattga tattgataat
      181 caagaaccaa taaaatcacc cgcgaacgat gaaaacaaaa aagtcggcca aaccatcatc
      241 aatgaaaaca tagtagagaa tactcatgat atcaataaca agaaaatgat cttcagcgca
      301 ataaaagaaa tatatgatga agatccaagt tttatcttcg ataaaatatc acacaaactc
      361 agacatacgg taacggaatt cgatgagagc gggaaaagcg aaccaacggg ct
//
LOCUS       DAZFFG010000442          409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFFG010000442 DAZFFG010000000
VERSION     DAZFFG010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.445"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>409
                     /locus_tag="ACHJ7J_005276"
     CDS             <1..>409
                     /locus_tag="ACHJ7J_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003331.1"
                     /translation="MALSALNLSNSGQWIAKNLTLKANSLTSAGDITGVDALTLTVNQ
                     TLNNHASGKLLSAGVLTLKADSVTNDGHLQGETLTLAASGGVNNRSGGVLMSRNALNV
                     STATLSNQGTIQGGGGVFLNATDRLQNDGKILSG"
BASE COUNT          102 a          109 c          124 g           74 t
ORIGIN      
        1 atggcgctca gtgcactgaa tttaagcaac agcggacaat ggattgcaaa aaatctgact
       61 ctgaaggcga actcactgac cagtgcgggt gacatcaccg gtgtggatgc tctcacgctc
      121 acggtgaatc agacgctgaa caatcacgcg agcggaaaac tgctcagtgc aggtgtgctg
      181 acgctgaagg cagacagtgt cacaaacgac gggcatctgc agggcgaaac gctgacgctg
      241 gccgcctccg gcggcgtgaa caaccgttcc ggtggtgttc tgatgagccg gaatgcactg
      301 aatgtcagta ctgcgaccct gagtaaccag ggcacgatac agggtggtgg cggggttttc
      361 ctgaacgcca ctgaccgtct gcagaacgac ggcaaaatcc tctccggca
//
LOCUS       DAZFFG010000443          400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFFG010000443 DAZFFG010000000
VERSION     DAZFFG010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.446"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            36..>400
                     /gene="espR1"
                     /locus_tag="ACHJ7J_005277"
     CDS             36..>400
                     /gene="espR1"
                     /locus_tag="ACHJ7J_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFO5003332.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLTTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNALESINFP"
BASE COUNT          157 a           83 c           56 g          104 t
ORIGIN      
        1 aaatcagaaa aacattgtat tactacggag cagttatgaa attcccttca atatttaaca
       61 aaataaaacc acaatccata cagcaacatc cagaaaaaaa tcaacttaac tggatgctcg
      121 aattaaataa atggaaagaa gaacgtatac ttacaggtga aatccatcgt ccggaatgtc
      181 gaaacgaagc cgctaaaagg ataaactgtg cttttttgtc gaaacagaat gacattgatt
      241 tatcaggact taatttaact actcaaccac cagggctgca aaacttcacc tctatcaatc
      301 ttgataataa ccaactcaca cattttgatg caaccaacta cgatagactc gtaaaactta
      361 gtctgaatag taacgctctt gagtcaataa attttcctca
//
LOCUS       DAZFFG010000444          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFFG010000444 DAZFFG010000000
VERSION     DAZFFG010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.447"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          134 a           81 c           73 g          108 t
ORIGIN      
        1 gtatcacgta aaaatatagt catcatagat gttatgcagt acgcacaaaa aaacagactg
       61 gatttgcttg ctgatctata atctttatct aaattactat tggcgctttg gataataaca
      121 taagcatata ttacctccta gcaataatag acataacctc atatcaaggt gtcactggta
      181 aataatcgga taaaagacag tatcggtcgg cgcatagagc ttcttctatt tcaccagaag
      241 tgcacagcag ggaactacca atcacacggt tagtgtcatg ggtggaggat gcgacccgtt
      301 ctttccctat aacggggata cgcagtttct atcagtacat gcagaaaacc cctaattcac
      361 atcactgcag aaagcattta atagaaatat acatcg
//
LOCUS       DAZFFG010000445          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFFG010000445 DAZFFG010000000
VERSION     DAZFFG010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.448"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          147 a           71 c           55 g          123 t
ORIGIN      
        1 ttataaaatg tatgaataac aacattaatt ttattctgct atccacagat tgatctatcg
       61 gccaattaga gaaaattgca taatccattt tcacaagcaa gagcagacat aaaattgatg
      121 gtagatagtc cgaaaaaaat attgtccgta tccattttaa catttgtttt ccccctacaa
      181 tgcaaggtat aaaatttgat gcaaatagaa cttttcatct tagccataaa agatataaca
      241 tttttagaga acaaaaatta aacctcatgt caatctataa cagcattcat ttactacctt
      301 ctggtacata cattcatcat tggttcatgg aggaagttat agcttcccgc ctacataaag
      361 agaaactaat actgctgcgg tagatagagc aactat
//
LOCUS       DAZFFG010000446          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFFG010000446 DAZFFG010000000
VERSION     DAZFFG010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.449"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..365
                     /locus_tag="ACHJ7J_005278"
     CDS             <1..365
                     /locus_tag="ACHJ7J_005278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003333.1"
                     /translation="GPLSSENVVFSLHGITNGANSIIITGQDDSVGVNGDTVAKDSNG
                     KEWSLTLYGSDITCENGGEFSFEDSSNFPGLITNDPSVYKCTGSNNTAGISINFRNEE
                     EWPTGSYTLKMSAQAWSE"
BASE COUNT          135 a           63 c           86 g          111 t
ORIGIN      
        1 ccggcccatt gagtagtgaa aatgttgtat ttagtttgca tggtattaca aatggcgcaa
       61 actctatcat cataactgga caagatgatt ctgtaggtgt taatggagat acagttgcta
      121 aagatagtaa tggtaaagaa tggagcttaa cattatatgg aagcgatata acctgtgaaa
      181 atggtggtga attctccttc gaggactctt ctaactttcc aggtctaata acaaatgatc
      241 catccgtata taaatgtaca gggagcaata atacagcagg aatatctata aactttagaa
      301 acgaagaaga atggccaaca ggttcatata ctttaaaaat gtcagctcag gcttggtctg
      361 agtaatagta aaccacctcc tcatgaggag gtggt
//
LOCUS       DAZFFG010000447          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFFG010000447 DAZFFG010000000
VERSION     DAZFFG010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.450"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(105..374)
                     /locus_tag="ACHJ7J_005279"
     CDS             complement(105..374)
                     /locus_tag="ACHJ7J_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HFO5003334.1"
                     /translation="MDKNYVFFNTHKNALIPGRVEDEHFWLLIGISTIHSKKVVMSMQ
                     DYLVHGIPRKNVCEKYKLSHSYFDNSLARLHRISCLITGLIKYYS"
BASE COUNT          141 a           61 c           36 g          143 t
ORIGIN      
        1 taaacacata gttaaaaatt tcactatatt taattctgat acgatgtatt tttttaataa
       61 aacaatcata taacactcac tatatagtac atgatttaat tatttcatga ataatacttt
      121 atcaaccctg taattaaaca acttattcga tgtaatctag ctaaactatt atcaaaataa
      181 ctatggctta acttatattt ttcacataca ttttttctcg ggattccgtg gacaagataa
      241 tcctgcatag acataacaac ttttttacta tgtatagtag atatacctat caataaccag
      301 aagtgttcat cttctaccct tcctggtata agggcatttt tatgagtgtt aaaaaaaaca
      361 taatttttat caataaatct a
//
LOCUS       DAZFFG010000448          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFFG010000448 DAZFFG010000000
VERSION     DAZFFG010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.451"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(149..367)
                     /gene="ymgF"
                     /locus_tag="ACHJ7J_005280"
     CDS             complement(149..367)
                     /gene="ymgF"
                     /locus_tag="ACHJ7J_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO5003335.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
BASE COUNT          124 a           79 c           73 g          102 t
ORIGIN      
        1 atagaaatta tttcaatgat atataaaaac aactgtgata acaccagggt tgacaactat
       61 ttgcgcaata attcctttcc ttcaatgatc caggcgttca ctgtcagcac cccattcagc
      121 cgcatactga cagtgagaaa ctgaaagatt attgctgatc cctgtggctg acgagtcttt
      181 cccttaatcg attaaccaga ttgatcgcca gccaaacaag ggcattaagc aaatttatcg
      241 tcacaagcac ttctcccagc ccggtactgg gttttgcaat aaggatgacc aggagagtga
      301 tcagcataaa tgcaatggaa aagatgatat agagagtgaa ataatccaga ttattactat
      361 tgttcatgct actaatgg
//
LOCUS       DAZFFG010000449          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFFG010000449 DAZFFG010000000
VERSION     DAZFFG010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.452"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..357
                     /locus_tag="ACHJ7J_005281"
     CDS             <1..357
                     /locus_tag="ACHJ7J_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5003336.1"
                     /translation="FRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIR
                     TFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSING
                     SSAEGYNSQATLNVTF"
BASE COUNT           79 a          112 c          103 g           81 t
ORIGIN      
        1 ttccgtctgg gcagccacaa cgatatgacc tttggtgaag gcacctcatc ccgtgccccc
       61 ctgcgtgaca gtgcaaaaca cagtgtgcgt gaactgccgg tgaactggtg ggtacagcct
      121 tccgttatcc gcaccttcag ctcccggggt gatatgcgtg tggggacctc caccgcaggc
      181 agcgggatga cgttctctcc ctcacagaat ggcacttcac tggatctgca ggccggactg
      241 gaagcccgtg tccgggaaaa tatcaccctg ggcgttcagg caggttatgc ccacagcatc
      301 aacggcagca gcgctgaagg ttataacagt caggccacac tgaatgtgac cttctgataa
      361 ttctgcattg cctct
//
LOCUS       DAZFFG010000450          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFFG010000450 DAZFFG010000000
VERSION     DAZFFG010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.453"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          124 a           61 c           64 g          122 t
ORIGIN      
        1 tagtgcttta ttttaacaaa agattcacct caggcatctt cattatattt gagcctatgg
       61 taggaaattc agtgagcagc acgtatacaa ggttatatct tcaccggatc attggctgta
      121 agtggtatct tattttagag tcagaaaaaa gacattggta cgataaaggg ctttcttata
      181 aacattgata caatttcacc tgcaaaaatt attatgctaa gtttcatcga acgccttgtc
      241 tctcgttctt tctctttttt caaaagacgg caatcctgtc ttacacagaa gattaaatct
      301 tcatgacaaa aatacaagtt atttcaagag aggtgcatat cagaaaataa tgattgagag
      361 taatagttaa g
//
LOCUS       DAZFFG010000451          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFFG010000451 DAZFFG010000000
VERSION     DAZFFG010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.454"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(135..>365)
                     /locus_tag="ACHJ7J_005282"
     CDS             complement(135..>365)
                     /locus_tag="ACHJ7J_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003337.1"
                     /translation="REISDNMLDSVKGGMNLNGLPGSTNVIDLRGKDMGTYIDANGAC
                     WAPDTPSIIMYPGGSGPSYSMSSSTSSANSGS"
BASE COUNT          106 a           81 c           71 g          107 t
ORIGIN      
        1 gtaatatatg aaaaggctct cgttaagatg tctggtacaa aatgtcagat catatgcctt
       61 tgtaaagaga cgacagaagt ggagtcttac aggcacatca taaataccct ccggcatagc
      121 cggagggtat ttatttaact gccgctgttt gcactggatg tggaactact catactataa
      181 gaaggtccac ttccccccgg atacatgatg atggatggag tatccggagc ccagcatgct
      241 ccattagcat caatatatgt tcccatatct tttccacgta gatctattac attagtagaa
      301 ccaggtaatc cattaagatt catccctcct ttcacggaat caagcatgtt atctgatatt
      361 tctct
//
LOCUS       DAZFFG010000452          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFFG010000452 DAZFFG010000000
VERSION     DAZFFG010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.455"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>365)
                     /locus_tag="ACHJ7J_005283"
     CDS             complement(<1..>365)
                     /locus_tag="ACHJ7J_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003338.1"
                     /translation="LMQVVSFIVDNNLTTKNIFSWRNPLKDERDYFVYDGRGTFKDKL
                     PDEIALNAFAEVFSRALTDKRDIFTTSVIVLLMSAPSRISEVFSLPVDCEIQQITRSG
                     DVKHGLRFWAGKGYGGDIK"
BASE COUNT          125 a           78 c           48 g          114 t
ORIGIN      
        1 tttaatatca ccaccataac ctttcccagc ccagaacctt aagccatgtt taacatctcc
       61 acttcttgta atctgctgta tttcacaatc tactggtaat gaaaaaactt cagaaattct
      121 tgatggcgca gacattaaaa gcacgataac agatgtagta aaaatatctc ttttatcagt
      181 taatgctcta ctaaagacct cagcaaatgc gttcaaagca atttcatctg gaagtttgtc
      241 tttaaaggtg cctctaccgt cataaacaaa ataatctcgc tcatccttta atgggtttcg
      301 ccacgaaaaa atatttttag tagtaagatt attatctaca ataaacgata caacttgcat
      361 taacg
//
LOCUS       DAZFFG010000453          362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFFG010000453 DAZFFG010000000
VERSION     DAZFFG010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.456"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>362
                     /locus_tag="ACHJ7J_005284"
     CDS             <1..>362
                     /locus_tag="ACHJ7J_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO5003339.1"
                     /translation="DIENILDGKGDLFKKRTLWEFVRDLFPGSHIKEVKGLIYEFVTK
                     VDNKAEVFDKIKSLAKTEQQWRFSTKTDFTTNENNEVIVSRSFNLYTGVTSNDNEKKQ
                     VSSERLTLDNYLDDSHFD"
BASE COUNT          127 a           53 c           72 g          110 t
ORIGIN      
        1 aggatattga aaatatcctt gatggcaaag gggatttatt taaaaaacgt acactgtggg
       61 agtttgttcg cgatcttttt cccggttctc atattaaaga agtcaaaggc ttaatttacg
      121 aatttgtcac taaagttgat aacaaggccg aggtgttcga taagattaaa tcgctggcaa
      181 aaacagaaca acaatggcga tttagtacta agacagactt taccacaaat gaaaataatg
      241 aagttattgt gagtagatcc tttaatctct acacgggggt tacttcgaat gataacgaaa
      301 aaaaacaggt ttcttcagag agattaacgc ttgataatta tcttgatgat tcacattttg
      361 at
//
LOCUS       DAZFFG010000454          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFFG010000454 DAZFFG010000000
VERSION     DAZFFG010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.457"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>360
                     /locus_tag="ACHJ7J_005285"
     CDS             <1..>360
                     /locus_tag="ACHJ7J_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5003340.1"
                     /translation="IVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETI
                     IKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMVEGVNFLS
                     QLLNIEDEVLNVLLRQNI"
BASE COUNT          112 a           65 c           68 g          115 t
ORIGIN      
        1 attgtttatt tacttaataa tatgacagta aatgataatg ctaatgatgt tgcagttact
       61 gagtcgtaca aacccatctg gaactggaaa atatcgcaat acaatgtatc tgatattaaa
      121 ttcgaaacaa ttataaaacc ccagttcgct gacagaatct atttctccaa ttgttcaccc
      181 gttgatccca caagtaccag accaacctat tttggtgata ccgatggctc agtcggtgca
      241 gttttgtacg cgcttttcgc aacgggacat ttgggaatta tggttgaagg ggtaaatttt
      301 cttagccagt tgcttaatat agaagatgaa gttctcaacg ttttattaag gcagaacatt
//
LOCUS       DAZFFG010000455          351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFFG010000455 DAZFFG010000000
VERSION     DAZFFG010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.458"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            138..344
                     /locus_tag="ACHJ7J_005286"
     CDS             138..344
                     /locus_tag="ACHJ7J_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO5003341.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          103 a           72 c           84 g           92 t
ORIGIN      
        1 aataaaccat caataagcaa aaataagtgg tcggatgagt agtaaaatta cacaaaaggc
       61 ggtactattt tcatcagata aacaaattca tttttccggt gctattcacc gggtagatac
      121 aaaaggggga gaaaagtatg gaactctaca gagaatatcc tgcatggctt atctttttac
      181 gccgtactta tgcggttgca gcgggcgttc tggcgctgcc tttcatgctt ttctggaaag
      241 accgcgcccg cttttacagc tacctgcatc gcgtctggtc gaaaaccagc gataaaccgg
      301 tgtggatgga tcaggccgaa aaggcaactg gtgattttta ctgaatgctg a
//
LOCUS       DAZFFG010000456          351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFFG010000456 DAZFFG010000000
VERSION     DAZFFG010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.459"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>351
                     /locus_tag="ACHJ7J_005287"
     CDS             <1..>351
                     /locus_tag="ACHJ7J_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 1"
                     /protein_id="HFO5003342.1"
                     /translation="VVFCIRKDWKERPGGDVIQLVETKNAIESAYKCSINIISDPDEI
                     LNIHPDIVHIFNMQTFEESKLFLTKAKQIGAFCVLSTVYWDMHDAFFVNAMQKMHIYP
                     SGKYFELLKRVFHLT"
BASE COUNT          114 a           49 c           69 g          119 t
ORIGIN      
        1 gtagttttct gtataaggaa agactggaag gagcgtcctg gaggagatgt gattcaattg
       61 gttgagacta agaacgcaat tgagtcagcg tataaatgct ctattaacat tatcagtgat
      121 ccggatgaaa ttttgaatat tcaccctgat attgtccaca tattcaatat gcaaactttt
      181 gaggaaagta agttatttct tacaaaagca aagcaaatcg gcgcattttg tgttctttct
      241 accgtttatt gggatatgca tgatgcattc tttgttaatg caatgcaaaa aatgcatatt
      301 tatcctagtg gaaaatattt tgaactttta aaaagagtat ttcatttaac g
//
LOCUS       DAZFFG010000457          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFFG010000457 DAZFFG010000000
VERSION     DAZFFG010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.460"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            53..>348
                     /locus_tag="ACHJ7J_005288"
     CDS             53..>348
                     /locus_tag="ACHJ7J_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO5003343.1"
                     /translation="MITDLILHNHPRMKKITLNDNHIAHLNTKTTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDSLAQMQYWTAVRNIIDDTNEVTIDLSYNL"
BASE COUNT          133 a           73 c           45 g           97 t
ORIGIN      
        1 gctggatgtc tcccggcaag aatctctgat tgagttaagt gcccatcaca atatgatcac
       61 tgaccttata ttacacaatc atcccaggat gaaaaaaatc actttaaacg acaaccatat
      121 tgcacattta aacaccaaaa ccactacaaa acttgaatat ttaaacttaa gcaataacaa
      181 tttattgcca accaatgaca ttgatcaact aatatcatca aaacatcttt ggcatgtatt
      241 agttaacggc atcaacaatg attcacttgc acaaatgcag tactggactg cagtaagaaa
      301 tataattgat gacactaatg aagtgaccat tgatttatct tataacct
//
LOCUS       DAZFFG010000458          342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFFG010000458 DAZFFG010000000
VERSION     DAZFFG010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.461"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>342
                     /locus_tag="ACHJ7J_005289"
     CDS             <1..>342
                     /locus_tag="ACHJ7J_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5003344.1"
                     /translation="QADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTL
                     NNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAV
                     NLNEGTLTLND"
BASE COUNT           92 a          104 c           95 g           51 t
ORIGIN      
        1 tcaggcggat gccctgatgc tggaaaaagg cagttcattc acgctgaacg ccggtgatac
       61 ggccacggat accacggtaa atggcggact gttcaccgcc aggggcggca cactggcggg
      121 caccaccacg ctgaataacg gcgccatact taccctttcc gggaagacgg tgaacaacga
      181 taccctgacc atccgtgaag gcgatgcact cctgcaggga ggctctctca ccggtaacgg
      241 cagcgtggaa aaatcaggaa gtggcacact cactgtcagc aacaccacac tcacccagaa
      301 agccgtcaac ctgaatgaag gcacgctgac gctgaacgac ag
//
LOCUS       DAZFFG010000459          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFFG010000459 DAZFFG010000000
VERSION     DAZFFG010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.462"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>333)
                     /locus_tag="ACHJ7J_005290"
     CDS             complement(<1..>333)
                     /locus_tag="ACHJ7J_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003345.1"
                     /translation="LGLKYAPVDGDGYCLLRAILVLKQHDYSWALGSHKTQKEVYEEF
                     IKIVDKQTIEALVDAAFYDLREDVKTLFGVDLQSDNKIQGVGSPMSWSFLFFKKQFID
                     SCLNNEKCI"
BASE COUNT          106 a           74 c           46 g          107 t
ORIGIN      
        1 gatacatttt tcgttattca agcaactatc aatgaattgt tttttaaaaa aaagaaagct
       61 ccatgacatg ggactaccca caccctgaat tttgttgtca gattgcagat caacgccaaa
      121 taatgtctta acatcttccc tgagatcata gaatgccgca tcaacaagag cctcgatcgt
      181 ttgtttatca acaattttaa taaactcttc gtatacttcc ttttgtgtct tatgactgcc
      241 cagcgcccat gaataatcat gttgttttaa aaccagtata gctcttaaca gacaatatcc
      301 atcgccatct acaggagcat attttaatcc taa
//
LOCUS       DAZFFG010000460          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFFG010000460 DAZFFG010000000
VERSION     DAZFFG010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.463"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          109 a           57 c           57 g          106 t
ORIGIN      
        1 cgtcagcgag aaatgaaaga agcgttaaat acactgaagg atctgagctc aatcagaaag
       61 ctggcaatta acaagtattt tacgggtgat tttgggcttt tgttacaaat tcgcaacgaa
      121 gaattgtgag ccattagccc aagaaaatta tattttaatg atttcaacag ttactagtgt
      181 gtttcttatt ataatcgtca agtaaaaaat ctttgtgttt ttcagccagt aaacctactt
      241 ccccccattt caaaatatgt gtccagtatc ccatgccgac agataatatt ggagcgtaaa
      301 aaataacaat tctatttttt gcattttcg
//
LOCUS       DAZFFG010000461          318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFFG010000461 DAZFFG010000000
VERSION     DAZFFG010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.464"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          104 a           66 c           53 g           95 t
ORIGIN      
        1 gttattttct gaaactctca gaataatttc aagattatat attagccatt ttgatgagta
       61 atttaggtta ttggtgaaca gagtggcgct ttctgctcgc taattccgca agcctgacaa
      121 aaccgaagtg aataaaaatg caatataaca gaataaaaga aaacacccct cactggtgct
      181 ttctgctgtt aatgtacatt ttctggcata aaaaatgctg taaatgaccc ggtcacgaca
      241 tacaccgaat tctctacgct tatagcgtta cctccccccc aaactttcat tagtgaaatt
      301 aaaggtaagg ccatatct
//
LOCUS       DAZFFG010000462          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFFG010000462 DAZFFG010000000
VERSION     DAZFFG010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.465"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..275
                     /locus_tag="ACHJ7J_005291"
     CDS             <1..275
                     /locus_tag="ACHJ7J_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003346.1"
                     /translation="NGNDYLSLGYYDQDGYHIQKGFRIDGDNLTQYCSKKARSASAWF
                     EHSKAIIAESFATGSDHQVVNELKGERLRELNEAFNPSNLARGYEY"
BASE COUNT          122 a           50 c           63 g           82 t
ORIGIN      
        1 aaaatggaaa tgattactta tctctcggtt actacgatca ggatggctac cacattcaaa
       61 aaggatttcg tattgatggt gataatctca cccaatattg tagtaaaaaa gccaggagtg
      121 cttctgcgtg gtttgaacac agtaaagcaa tcattgcaga atcatttgca acaggttccg
      181 atcatcaagt tgtaaacgaa ctcaaagggg aaagactgag agaacttaac gaagctttta
      241 atccttcaaa tctagcaaga ggatatgaat attaagagga tgatagaaaa tatacacttg
      301 agaatacaaa agaaata
//
LOCUS       DAZFFG010000463          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFFG010000463 DAZFFG010000000
VERSION     DAZFFG010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.466"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>317)
                     /locus_tag="ACHJ7J_005292"
     CDS             complement(<1..>317)
                     /locus_tag="ACHJ7J_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5003347.1"
                     /translation="LYVIGQYCDQSVPDGSGGTEPRITCNAYLTTQRKAWDVLSDFCS
                     AMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVN
                     WIDP"
BASE COUNT           64 a          103 c           88 g           62 t
ORIGIN      
        1 gggtcaatcc agttcacctc aacggcatta tggcggtcct tcagggcgct gaagctgtag
       61 cggaacggcg cgccatcatc cggcatcacc acattactgc ggttataggt ccacacctta
      121 tccgacggtc ggtcctgcac gaacgtcagc gtctgcccgt tccataccgg catacagcgc
      181 atcgccgagc agaaatcact gagcacatcc cacgccttgc gctgtgtggt caggtacgca
      241 ttacaggtga tgcgcggctc cgtgccgcca gagccgtccg gcactgactg gtcgcagtac
      301 tggccgatga catacag
//
LOCUS       DAZFFG010000464          304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFFG010000464 DAZFFG010000000
VERSION     DAZFFG010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.467"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>304)
                     /locus_tag="ACHJ7J_005293"
     CDS             complement(<1..>304)
                     /locus_tag="ACHJ7J_005293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase 2 family protein"
                     /protein_id="HFO5003348.1"
                     /translation="QNADNFSIDIYVRDDGSNNVNLVNLRQMTSCLPINVFEGSNIGV
                     TKSFFTLLEQVDSYDYYAFCDQDDVWLDDKISSAVTMMKDSEGPVLYCSAYTLVDA"
BASE COUNT          110 a           65 c           42 g           87 t
ORIGIN      
        1 tttgcatcaa ccaaggtata agctgaacag tataatactg gaccttcgga atccttcatc
       61 atggtcactg cgcttgaaat tttatcatca agccaaacat catcctgatc gcaaaaggca
      121 taataatcat atgaatcaac ctgctccaag agagtaaaaa aacttttagt cacacctata
      181 ttgcttccct caaaaacatt aataggtaaa caggatgtca tttgccgaag gtttaccaaa
      241 ttaacattat tagaaccatc atccctgaca tatatatcaa tagaaaaatt atctgcattc
      301 tggt
//
LOCUS       DAZFFG010000465          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFFG010000465 DAZFFG010000000
VERSION     DAZFFG010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.468"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            23..>294
                     /locus_tag="ACHJ7J_005294"
     CDS             23..>294
                     /locus_tag="ACHJ7J_005294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003349.1"
                     /translation="MNEHANIGMRMLAVAVKKKNGGADVAGEPYKMQIKDYQYDNQGK
                     ISNDSKYSYSYADMMSTSPFFNPFLIRKHCIIGAEILCRVQTQNSY"
BASE COUNT          109 a           44 c           60 g           81 t
ORIGIN      
        1 atacacagca tggaaataga tattgaatga gcatgccaat attggtatgc gaatgctggc
       61 ggtagcagta aaaaagaaaa atggtggggc ggatgtagca ggagagccat ataaaatgca
      121 aataaaagac taccaatatg ataatcaagg aaagatcagt aatgattcca aatattcata
      181 ttcatatgct gacatgatgt cgacttctcc ttttttcaac ccatttttga tacgaaagca
      241 ttgtattatt ggtgctgaaa tcttatgcag agttcaaacg caaaacagtt atat
//
LOCUS       DAZFFG010000466          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFFG010000466 DAZFFG010000000
VERSION     DAZFFG010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.469"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            1..190
                     /locus_tag="ACHJ7J_005295"
                     /pseudo
     CDS             1..190
                     /locus_tag="ACHJ7J_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
BASE COUNT           77 a           77 c           61 g           79 t
ORIGIN      
        1 cccgctgccg ttcattctct cccgcaccta cagcagttac cggacaaaaa cgcccgcgcc
       61 ggtggggagc ctcggccccg gctggaaaat gcctgcggat atccgcttac agctgcgcga
      121 taacacactg atactcagtg ataacggcgg cagaagcctg tattttgagc acctgtttcc
      181 agggaaagat catcagaagt acactttttg tactaaataa ttcgcatttt atgtttaaaa
      241 attgagatat tcctcattac ctaaagctgt cttttattgc ttatacatga tcaa
//
LOCUS       DAZFFG010000467          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFFG010000467 DAZFFG010000000
VERSION     DAZFFG010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.470"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            163..>294
                     /locus_tag="ACHJ7J_005296"
     CDS             163..>294
                     /locus_tag="ACHJ7J_005296"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO5003350.1"
                     /translation="MNKLISACAGAGKTHRIVSEAIQAIDKGERILIITYTINNQKEI
                     "
BASE COUNT          112 a           43 c           53 g           86 t
ORIGIN      
        1 tttatgataa attaacgaca cttgatgaaa aaattgcttt tttgaaaaca tggtataacg
       61 gagatgatgg tgcaggtaag aaaaaagtag attccgcaat cagaatattt gactctgagg
      121 atagcattct ttacccacat tttttcaagg atgttttcaa tcatgaataa attaattagc
      181 gcatgtgcag gtgcaggaaa aacccaccgc attgttagtg aggctattca agccattgac
      241 aaaggagaac gtattttaat cattacatat acaataaata accaaaaaga aatt
//
LOCUS       DAZFFG010000468          293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFFG010000468 DAZFFG010000000
VERSION     DAZFFG010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.471"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>293
                     /locus_tag="ACHJ7J_005297"
     CDS             <1..>293
                     /locus_tag="ACHJ7J_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO5003351.1"
                     /translation="KVPDWPGMYEQQGENLNGQLEQYEIFDYPGRFKDEQHGKDFTLY
                     QMESLRSDAEKATGQSNSPKLWPGTRFTLTGHPQKMLNREWQVVQSILSGSQP"
BASE COUNT           79 a           64 c          104 g           46 t
ORIGIN      
        1 aaggtgccgg actggccggg gatgtatgag cagcagggtg agaacctgaa cggacagctg
       61 gagcagtacg agatatttga ttatccggga aggttcaagg acgagcagca cggcaaggat
      121 ttcacgctgt accagatgga gagcctgcgc agtgatgcgg aaaaggccac agggcagagt
      181 aattcaccga agctgtggcc gggaacgcgg tttacgctga cggggcatcc gcagaagatg
      241 ctgaaccgtg aatggcaggt ggtacagagc atactgtcag gaagccagcc gca
//
LOCUS       DAZFFG010000469          291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFFG010000469 DAZFFG010000000
VERSION     DAZFFG010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.472"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT           85 a           85 c           45 g           76 t
ORIGIN      
        1 cacaccctga aagtaacaat gcgaaaacca ggagcctatc ccaacatgat taggccgctg
       61 gtaatataca gcagagcctt ataacttcag ttcttttccc atcagctcag tatctgacga
      121 aaacaatgaa tccagctata aaccctacct actatccaca ggcgagcttt gcctccttct
      181 ggtatcttcc cctgtttact cgactgaata tgaggttcac agcatcgctg cctccagata
      241 attgctcaac cattcgtcat cagcaacctt ttcactttga aaacaacatc g
//
LOCUS       DAZFFG010000470          289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFFG010000470 DAZFFG010000000
VERSION     DAZFFG010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.473"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..>289)
                     /gene="wzy"
                     /locus_tag="ACHJ7J_005298"
     CDS             complement(<1..>289)
                     /gene="wzy"
                     /locus_tag="ACHJ7J_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158642113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O154 family O-antigen polymerase"
                     /protein_id="HFO5003352.1"
                     /translation="TTIIIILSSSVEYFKLGIPLFGHGIYAEFGYPFLHHVSVMSWLL
                     LFTCNSFKNRIIFILVMVFAFVNPLLMINRDLMLLTCFVFIFILLGKRKVSL"
BASE COUNT          131 a           46 c           32 g           80 t
ORIGIN      
        1 tagactaacc tttcgttttc ctaaaaggat aaaaataaaa acaaaacaag ttaatagcat
       61 taaatcacga ttaatcatca acaatggatt aacaaatgca aaaaccatta caagtataaa
      121 aattattcta ttcttaaagg aattacatgt aaataataat aaccatgaca taactgaaac
      181 atggtgcaaa aaaggatacc caaactctgc atatatacca tgtccaaaaa gaggaattcc
      241 taacttaaaa tattcaacac tgcttgatag aattataatg attgttgtc
//
LOCUS       DAZFFG010000471          288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFFG010000471 DAZFFG010000000
VERSION     DAZFFG010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.474"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>288
                     /locus_tag="ACHJ7J_005299"
     CDS             <1..>288
                     /locus_tag="ACHJ7J_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO5003353.1"
                     /translation="TACVKNNASYQFNNALPNKETISSNFCERLEQWGNKLLNNGEER
                     AIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFT"
BASE COUNT           98 a           51 c           52 g           87 t
ORIGIN      
        1 tacagcttgt gtaaaaaata atgccagtta tcaatttaat aacgcattac ctaacaaaga
       61 aaccatctcc agcaattttt gtgaacgact agaacaatgg ggtaataagt tgcttaataa
      121 tggtgaagaa agagcaattg ccgtggagcg aattaaagaa gcttacaatt cgaacatggc
      181 atctcttgat ttgtcatatc ttgatttgag tgagttaccg cctatcccct cgacagttaa
      241 tacgttaaat ctggaaaaca attgtctaac ttgtcttgac tttactga
//
LOCUS       DAZFFG010000472          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFFG010000472 DAZFFG010000000
VERSION     DAZFFG010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.475"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>278
                     /locus_tag="ACHJ7J_005300"
     CDS             <1..>278
                     /locus_tag="ACHJ7J_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5003354.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRL
                     TTIQNDRTRIQTIYQPGSFTPLIRVETATGELARTQRRSLADALQQSGG"
BASE COUNT           63 a           83 c           90 g           42 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtatggcg acgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccggaag tgacctggta cggctgggat
      121 ggcgaccgcc tgaccacgat acagaacgac agaacccgca tccagacgat ttatcagccg
      181 gggagcttca caccactcat cagagttgaa actgccaccg gtgaactggc cagaacgcag
      241 cgccgcagcc tggcggatgc gcttcagcag tccggcgg
//
LOCUS       DAZFFG010000473          272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFFG010000473 DAZFFG010000000
VERSION     DAZFFG010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.476"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>272
                     /locus_tag="ACHJ7J_005301"
     CDS             <1..>272
                     /locus_tag="ACHJ7J_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFO5003355.1"
                     /translation="VDNVKLIPPQEYLPFVYLMERSYIILTDSGGIQEEAPSLGKPVL
                     VMRETTERLEALNAGTVKLVGTNYDTIIKEFEYLMNDKSHYESMSKS"
BASE COUNT           91 a           41 c           50 g           90 t
ORIGIN      
        1 gttgataatg taaaattaat tccacctcaa gaatatcttc catttgttta tttaatggaa
       61 cgttcatata ttatcttaac tgattcagga ggtattcaag aagaagctcc ttccttgggc
      121 aaacctgttc tcgttatgcg tgaaactact gaaaggctag aagctttaaa tgcgggtacc
      181 gtgaagttag ttggtacaaa ttatgatact atcattaagg aatttgagta tctaatgaac
      241 gataagtcac attatgaaag tatgtcaaaa tc
//
LOCUS       DAZFFG010000474          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFFG010000474 DAZFFG010000000
VERSION     DAZFFG010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.477"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>270
                     /locus_tag="ACHJ7J_005302"
     CDS             <1..>270
                     /locus_tag="ACHJ7J_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO5003356.1"
                     /translation="SMSIRYRSKYYSRDSALIEEETIFSDAELKAILPMRRMYGVGDY
                     KSNSSSLSSLSGLKDPTGTPVCYYIHNDSKPSLGYGPTCNNWLSQS"
BASE COUNT           80 a           61 c           47 g           82 t
ORIGIN      
        1 ctcaatgtct attcgttata gatcaaaata ttattccaga gattccgcct taatagaaga
       61 agaaacaata ttttctgacg cagaactaaa agctattctg cctatgcgtc gcatgtacgg
      121 ggttggtgac tataagtcaa attcctcttc tctatcctca ctctcggggc tcaaggaccc
      181 aacgggcaca cccgtctgtt attatattca taatgatagt aaaccttcct taggttatgg
      241 tccaacatgc aataattggt taagccaatc
//
LOCUS       DAZFFG010000475          268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFFG010000475 DAZFFG010000000
VERSION     DAZFFG010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.478"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
BASE COUNT          104 a           31 c           62 g           71 t
ORIGIN      
        1 tattcacttg gcacaagagc tgcccaagaa ggatatagtg agtatatcat agcgaagctt
       61 ttggatcata gatcaacgag acttgttagc tgttatgtaa aaaatgtacc agagtatgca
      121 gaaaggattg atgaggttat gacaacggaa gttataaagt atgtgaatgc atttaaaggg
      181 catattataa ataaaagtga tgctcaaaag aaaataaaaa atcacgaggg tatagattca
      241 ggaaactgtt caaactgtaa gagttgtg
//
LOCUS       DAZFFG010000476          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFFG010000476 DAZFFG010000000
VERSION     DAZFFG010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.479"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            71..>267
                     /gene="dinJ"
                     /locus_tag="ACHJ7J_005303"
     CDS             71..>267
                     /gene="dinJ"
                     /locus_tag="ACHJ7J_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO5003357.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNS"
BASE COUNT           76 a           65 c           64 g           62 t
ORIGIN      
        1 tagattgtcg ctgttgctca tttgagctac aattcaagct gaataaatat acagcacagg
       61 agatacccca atggctgcta acgcgtttgt tcgcgcccga atcgatgaag atctgaagaa
      121 tcaggcggcg gacgtactgg ccgggatggg gctgaccatc tctgacctgg ttcgcataac
      181 cctcacaaag gtcgcgcgtg aaaaggcatt gccgtttgat ttacgcgagc ctaatcaatt
      241 aaccattcaa tcaatcaaaa acagcga
//
LOCUS       DAZFFG010000477          260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFFG010000477 DAZFFG010000000
VERSION     DAZFFG010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.denovo.480"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..>260
                     /locus_tag="ACHJ7J_005304"
     CDS             <1..>260
                     /locus_tag="ACHJ7J_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5003358.1"
                     /translation="VVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLE
                     LVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKIL"
BASE COUNT           57 a           63 c           81 g           59 t
ORIGIN      
        1 gtagttgctg cgactgacgg cccgatgccg cagactcgtg agcacatcct gctgggtcgt
       61 caggtaggcg ttccgtacat catcgtgttc ctgaacaaat gcgacatggt tgatgacgaa
      121 gagctgctgg aactggttga aatggaagtt cgtgaacttc tgtctcagta cgacttcccg
      181 ggcgacgaca ctccgatcgt tcgtggttct gctctgaaag cgctggaagg cgacgcagag
      241 tgggaagcga aaatcctgga
//
LOCUS       DAZFFG010000478         9643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.guided.153, whole genome shotgun sequence.
ACCESSION   DAZFFG010000478 DAZFFG010000000
VERSION     DAZFFG010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.guided.153"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            complement(<1..186)
                     /locus_tag="ACHJ7J_005305"
     CDS             complement(<1..186)
                     /locus_tag="ACHJ7J_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5003359.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGA"
     gene            complement(405..1382)
                     /gene="epmA"
                     /locus_tag="ACHJ7J_005306"
     CDS             complement(405..1382)
                     /gene="epmA"
                     /locus_tag="ACHJ7J_005306"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5003360.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1707..3515
                     /gene="frdA"
                     /locus_tag="ACHJ7J_005307"
     CDS             1707..3515
                     /gene="frdA"
                     /locus_tag="ACHJ7J_005307"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5003361.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3508..4242
                     /gene="frdB"
                     /locus_tag="ACHJ7J_005308"
     CDS             3508..4242
                     /gene="frdB"
                     /locus_tag="ACHJ7J_005308"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5003362.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4253..4648
                     /gene="frdC"
                     /locus_tag="ACHJ7J_005309"
     CDS             4253..4648
                     /gene="frdC"
                     /locus_tag="ACHJ7J_005309"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5003363.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4659..5018
                     /gene="frdD"
                     /locus_tag="ACHJ7J_005310"
     CDS             4659..5018
                     /gene="frdD"
                     /locus_tag="ACHJ7J_005310"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5003364.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5081..6214
                     /locus_tag="ACHJ7J_005311"
     CDS             5081..6214
                     /locus_tag="ACHJ7J_005311"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5003365.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQINLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6303..6836
                     /gene="blc"
                     /locus_tag="ACHJ7J_005312"
     CDS             6303..6836
                     /gene="blc"
                     /locus_tag="ACHJ7J_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5003366.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6833..7150)
                     /gene="sugE"
                     /locus_tag="ACHJ7J_005313"
     CDS             complement(6833..7150)
                     /gene="sugE"
                     /locus_tag="ACHJ7J_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5003367.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7326..7472)
                     /gene="ecnB"
                     /locus_tag="ACHJ7J_005314"
     CDS             complement(7326..7472)
                     /gene="ecnB"
                     /locus_tag="ACHJ7J_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5003368.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7583..7708)
                     /gene="ecnA"
                     /locus_tag="ACHJ7J_005315"
     CDS             complement(7583..7708)
                     /gene="ecnA"
                     /locus_tag="ACHJ7J_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5003369.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7760..8326)
                     /gene="efp"
                     /locus_tag="ACHJ7J_005316"
     CDS             complement(7760..8326)
                     /gene="efp"
                     /locus_tag="ACHJ7J_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5003370.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8368..9396
                     /gene="epmB"
                     /locus_tag="ACHJ7J_005317"
     CDS             8368..9396
                     /gene="epmB"
                     /locus_tag="ACHJ7J_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5003371.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2450 a         2480 c         2471 g         2242 t
ORIGIN      
        1 cgctcccatt tcagccatca ttaaggcgaa tggaataaag aataacaatg cagaaaatat
       61 ataaaaggga atcgcactat aacccattaa gtaataagcc gatgggctat tggcaaatcc
      121 aaatacggaa gtaaagatca tcaatatgag tcctatcaga ctcatctttt ttatcgtgtg
      181 aggcatgaaa ccatccttca tttcagcgcg tgtataaaac gcgcccactg aactgcactg
      241 cccaacattg agcgggcagc gtaaaaacca atacagaatt gatacctgga tgatatcaaa
      301 tatccagtct taaattgtgg cctttagcgg aaaaatgaaa atatatgcta cagaatggcg
      361 cttatcacgc cattcttcgc tgttaattca gtaatttttc agaattatgc ccggtcaacg
      421 ctaaaggcga tgacttcagc cagtgtctcc gcacccagcg ccaacatcac cagacgatca
      481 acgcccaatg ccacgccgga acagtcaggc ataccgactt tcaaggcttc aatcagattc
      541 tggtcaatgg ggtgctgcgg caaaccacgc gccgcgcgct tacggttatc ttgttcaaag
      601 cgttgttgct gctcacgggc atccgtcaat tcatggaaac cattcgccag ctcaatacct
      661 ttataataaa cctcaaagcg ttcagcgacc cgatgatctt cggtactgat ttgcgccagt
      721 gatgcctggc tggctggaaa gtggtacaca aaggtcggtt tttctttacc aatatttggc
      781 tctacgccaa aggtaaacag caattgtagc aacgtgtcgc ggtcttcttc ggtatcagca
      841 acgttgctca aatccagttt cgctgcgact tcccgcagtt gcgttttgtc ggcagagagc
      901 gggtcaattt ccagataacg caagaaagct tgttgataag aaaggctttc tgctgccggg
      961 cagtccagca cctgttgtaa gaggtcgtcc acctcgttca tcaaccggta catatcatag
     1021 tgcggtcgat accactccag catagtgaac tcagggttgt gataacgccc catctcttca
     1081 ttacggaaac tgcggcacag ctggaatacc ggcccgcaac cggcaaccag caggcgtttc
     1141 atatggtatt ccgggctggt cattaaccag agattcatcc cctgcgaatg cccggggcca
     1201 acgaaacgtg tctcaaacgg gaccaaatga atatcggtta ccgtcgcctg gctcatacaa
     1261 ggcgtctcca cctccagcac tccacgatcg gcaaagaaac gacggatctc cgccataatc
     1321 gccgcgcgtt ttaataagtt aggaatggat gcgctcggct gccaggatgc cgtttcgctc
     1381 atagttaaat ctccagtttt tgacaagggc acgaagtcta ctcgcaacgc gacggcgaaa
     1441 caaattttac gcaggaataa aacaacggtg ggcagtgact aaaaaaagca cgatctgatg
     1501 gtttagtaat taaattaatc atcttcagtg ataatttagc cctcttgcgc actaaaaaaa
     1561 tcgatctcgt caaatttcag acttatccat cagactatac tgttgtacct ataaaggagc
     1621 agtggaatag cgttcgcaga ccgtaacttt caggcactta ccctgaagta cggggctgtg
     1681 ggataaaaac aatctggagg aatgtcgtgc aaacctttca agccgatctt gccattgtag
     1741 gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc gcaggcaaat ccaaatgcaa
     1801 aaatcgcact aatctcaaaa gtatacccga tgcgtagcca taccgttgct gcagaagggg
     1861 gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata tcactttcac gatacagtag
     1921 cgggtggcga ctggttgtgt gagcaggatg tcgtggatta tttcgtccac cactgcccaa
     1981 ccgaaatgac ccaactggaa ctgtgggggt gcccatggag ccgtcgcccg gatggtagcg
     2041 tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgtac ctggttcgcc gccgataaga
     2101 ccggcttcca tatgctgcac acgctgttcc agacctctct gcaattcccg cagatccagc
     2161 gttttgacga acatttcgta ctggatattc tggttgatga tggtcatgtt cgcggcctgg
     2221 tagcaatgaa catgatggaa ggcacgctgg tgcagatccg tgctaacgcg gtcgttatgg
     2281 ctaccggcgg tgcgggtcgc gtttatcgtt acaacaccaa cggcggcatc gttaccggtg
     2341 acggtatggg tatggcgcta agccacggcg ttccgctgcg tgacatggaa ttcgttcagt
     2401 atcacccaac cggtctgcca ggttccggta tcctgatgac cgaaggctgc cgcggtgaag
     2461 gtggtattct ggtcaacaaa aatggctacc gttatctgca agattacggc atgggcccgg
     2521 aaactccgct gggcgagccg aaaaacaaat atatggaact gggtccacgc gacaaagttt
     2581 ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat ctccacgccg cgtggcgatg
     2641 tggtttatct cgacctgcgt cacctcggcg agaaaaaact gcatgaacgt ctgccgttca
     2701 tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt taaagaaccg attccggtac
     2761 gtccgaccgc acactacacc atgggcggta tcgaaaccga tcagaactgt gaaacccgca
     2821 ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg tctgcacggt gcaaaccgtc
     2881 tgggttctaa ctccctggcg gaactggtgg tcttcggccg tctggccggt gaacaagcga
     2941 cagagcgtgc agcaactgcc ggtaatggca acgaagcggc aattgaagcg caggcagctg
     3001 gcgttgaaca acgtctgaaa gatctggtta accaggatgg cggcgaaaac tgggcgaaga
     3061 tccgcgacga aatgggcctg gctatggaag aaggctgcgg tatctaccgt acgccggaac
     3121 tgatgcagaa aaccatcgac aagctggcag agctgcagga acgcttcaag cgcgtgcgca
     3181 tcaccgacac ttccagcgtg ttcaacaccg acctgctcta caccattgaa ctgggccacg
     3241 gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc acgtaaagag tcccgcggcg
     3301 cacaccagcg tctggacgaa ggttgcaccg agcgtgacga cgtcaacttc ctcaaacaca
     3361 ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga gtacagcgac gtgaagatta
     3421 ctacgctgcc gccagctaaa cgcgtttacg gtggcgaagc ggatgcagcc gataaggcgg
     3481 aagcagccaa taagaaggag aaggcgaatg gctgagatga aaaacctgaa aattgaggtg
     3541 gtgcgctata acccggaagt cgataccgca ccgcatagcg cattctatga agtgccttat
     3601 gacgcaacta cctcattact ggatgcgctg ggctacatca aagacaacct ggcaccggac
     3661 ctgagctacc gctggtcctg ccgtatggcg atttgtggtt cctgcggcat gatggttaac
     3721 aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt acaccgacgg tatgaaggtt
     3781 gaagcgttag ctaacttccc gattgaacgc gatctggtgg tcgatatgac ccacttcatc
     3841 gaaagtctgg aagcgatcaa accgtacatc atcggcaact cccgcaccgc ggatcagggt
     3901 actaacatcc agaccccggc gcagatggcc aagtatcacc agttctccgg ttgcatcaac
     3961 tgtggtctgt gctacgccgc gtgcccgcag tttggcctga acccagagtt catcggtccg
     4021 gctgccatta cgctggcgca tcgttataac gaagatagcc gcgaccacgg taagaaggag
     4081 cgtatggcgc agttgaacag ccagaacggc gtatggagct gtactttcgt gggctactgc
     4141 tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa
     4201 agttcgaaag actttcttat cgcgaccctg aaaccacgct aaggagtgca acatgacgac
     4261 taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat tgccgtttta
     4321 tcgcttttac atgctgcgcg aaggcacggc ggttccggct gtgtggttca gcattgaact
     4381 gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt
     4441 tttacaaaac ccggttattg tgatcattaa cctgatcact ctggcggcag ctctgctgca
     4501 caccaaaacc tggtttgaac tggcaccgaa agcggccaat atcattgtaa aagacgaaaa
     4561 aatgggacca gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt
     4621 aatcctgttt gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc
     4681 gttctgacga accggtattc tggggcctct tcggggccgg tggtatgtgg agcgccatca
     4741 ttgcgccggt gatgatcctt ctggtgggta ttctgctgcc actggggctg tttccgggtg
     4801 atgcactgag ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc
     4861 tgttcctgat gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc
     4921 acgatctgaa aatccacgta cctgcgggca aatgggtttt ctacggtctg gctgctatcc
     4981 tgacagttgt cacgctgatt ggtgtcgtta caatctaacg catcgccaat gtaaatccgg
     5041 cccgcctatg gcgggccgtt ttgtatggaa accagaccct atgttcaaaa cgacgctctg
     5101 cgccttatta attaccgcct cttgctccac atttgctgcc cctcaacaaa tcaacgatat
     5161 tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atcccgggta tggcggtggc
     5221 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa
     5281 gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg
     5341 cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa
     5401 atactggcct gaacttaccg ctaaacagtg gaacgggatc acgctattac atctcgcaac
     5461 ctacaccgct ggcggcctgc cattgcaggt gccggatgag gtgaaatcct caagcgactt
     5521 gctgcgcttc tatcaaaact ggcagcctgc atgggcgcca ggaacacaac gtctgtatgc
     5581 caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
     5641 gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt
     5701 accgcccgca gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt
     5761 ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc
     5821 ccgctgggtg caaatcaatt taaaacccct tgatatcaat gagaaaacgc ttcaacaagg
     5881 gatacaactg gcacaatctc gctactggca aaccggcgat atgtatcagg gtctgggctg
     5941 ggaaatgctg gactggccgg taaatcctga catcatcgtt aacggcagtg acaataaaat
     6001 tgcactggca gcacgccccg taaaagcgat tacgccccca actcctgcag tacgcgcatc
     6061 atgggtacat aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt ttattccaga
     6121 aaaagagctg ggtatcgtga tgctggctaa caaaaactat cccaatccag cgagagtcgc
     6181 cgccgcctgg cagattctca acgctctgca gtaaaatttc atcgggtccg aattttcgga
     6241 ccttttctcc gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat
     6301 ttatgcgcct gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgca
     6361 gttctcctac gccgccgcgt ggcgtgaccg tagtgaataa tttcgacgct aaacgctatc
     6421 tcggtacctg gtatgagatt gcccgttttg atcaccgctt tgaacgtgga ctggaaaaag
     6481 tcaccgcaac atacagcctg agggatgatg gcggcctgaa tgtcattaat aaaggctata
     6541 accctgacag agaaatgtgg cagcagagtg aagggaaagc gtactttacc ggcgcaccaa
     6601 ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta tggcggttat aacgttattg
     6661 cactcgatcg ggaataccgc catgcgctgg tttgcggccc ggaccgcgac tacctgtgga
     6721 tactctcccg cacgccaacc atttctgacg aagtgaaaca ggagatgctg gcagtcgcga
     6781 cccgggaagg gtttgatgtc agtaagttta tttgggtaca gcagcccggt agttaatgag
     6841 tgctgagttt cagaccaata atccccaata caattaacgc cagactcgcc aggcgcatcg
     6901 ggttagcgga ctcaccgagc agcacaatgc cggtgatggc cgcgccgact gcgccaatac
     6961 ccgtccacac ggcataagcc gtccctacag gtaatgactt catcgcccag gcaagtagcg
     7021 ccatactgac aatcatcgcc gtcacggtaa taacacttgg cgtcaaacga ctaaagccgt
     7081 gggtatattt caggccaacg gcccatacca cttcgagcag accagcaata actaagataa
     7141 tccaggacat atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga ggcgtttgca
     7201 ggtcgtcctg caaagctgat ggttgaacgg atattttgcc aggtggcagg ggaatttcaa
     7261 ctattacctt agtgactatc gcagaaaaaa caaacggcac gacacagctg tcgtaccgtt
     7321 attgcttatt gctgcgcttt cgttgctgca ccagaaatcg cgttaccgcc atcagaaatg
     7381 tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata ctgttgaaag caccagaaca
     7441 gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt ccttttaggt aatgttattt
     7501 atgtttgcct atagcaaatt aataatagac aacatcgcca aaaatgatgg aaatctgatg
     7561 atttttcgga agagaagaaa aattagctgg cagcgcggga gatggagttg ccgagatgtt
     7621 tgatgtcttc gccgaaaccg cgagcggtgt tacagcccgt gagcaacgtg ctggcaagca
     7681 aaacaagaac gataaggcgt ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc
     7741 aggccgcacc acaaccgcat tacttcacgc gagagacgta ttcaccagag cgggtatcca
     7801 ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt aaccacagcg ccagtagaca
     7861 gggtagccgg tttgccgcca gtacctgcgg tatcaccttt caggcccgga tcggtatcaa
     7921 cgatttccag ttcaacgaag ttcggcggag taacggagat cggctgacca ttccacagag
     7981 ttacgataca ctctgcctga tccagcagcc atttagcgtt gtcgccaatt gcttttgcgt
     8041 cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt
     8101 acaggtaagt caggttcata tcgacaacat cagcgccttc agcggaatca gtagatttga
     8161 aggttttttc tacgcgagta ccggtcagca gacgacgcag tttaacgcga gcaaatgcct
     8221 ggcctttacc cggttttacg aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca
     8281 tgattttaag accagcacga aaatcgttgc tatagtacgt tgccataagg ccctctgaaa
     8341 tttgttaatt ggtagctaag ccacaaaatg gcgcatattg taaccctaaa taccccatcc
     8401 agagaagatt ggttaacgca acttgccgat gttgtgaccg atcctgatga acttctgcgt
     8461 cttttgaata tagacgcgga cgaaaaactg ttggccggac gcaacgccaa aaagctgttt
     8521 gccctgcgtg tgccccgctc atttatcgat cgcatggaga aaggcaatcc ggacgatccg
     8581 cttttgcgtc aggtaattac ctcgcaagat gagtttgcca tcgcgcccgg attctccacc
     8641 gacccgctgg aagaacagca cagcgtagtg cctggtttgt tgcataaata ccacaaccgg
     8701 gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc gttattgctt ccgtcgccac
     8761 ttcccttatg cagaaaatca gggcaacaag cgtaactggc aaactgcact tgagtatgtt
     8821 gctgcgcatc cggaactgga cgagatgatt ttctccggcg gcgatccgct gatggcgaaa
     8881 gatcacgagc tggactggtt gctcacacaa ctggaagcca tcccgcatat caaacgtctg
     8941 cggattcaca gtcgtctgcc gattgtgatc ccggcacgca tcacagatgc gctggttgaa
     9001 cgctttgccc gttctacgct gcaaatcttg ctggtgaatc acatcaatca tgccaatgag
     9061 gtagatgaaa cattccgtca ggcgatggct aagttgcgcc gggtaggcgt tactttgctg
     9121 aaccagagcg ttctgttacg tgatgtgaac gataacgcac aaacgctggc aaacctgagt
     9181 aatgcgttgt tcgatgccgg cgtaatgccg tattacctgc atgtgctcga taaagtacag
     9241 ggcgcggcgc attttatggt gagtgatgac gaagcgcggc agattatgcg tgagttgctg
     9301 acactggtgt cgggatatct ggtgccgaaa ctggcgcgag aaattggcgg cgaacccagc
     9361 aaaacgccgc tggatctcca gctacgccag cagtaactga ttaaacaggg aagtcaaaca
     9421 ttaaaattgc ctgatgcgct acgcttatca ggcctacatc gtgcattgaa attgaatgga
     9481 tttgcaatga actgtaggcc ggataaggcg tttacgccgc atccggcaag agcaacaaac
     9541 gcaagtacag caacctgtca ttaaacaggg aagtttaatc tttgcccgtt gcataacctt
     9601 taattattca tatgcattgc tgttgttact ctttatataa cta
//
LOCUS       DAZFFG010000479         3476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.guided.246, whole genome shotgun sequence.
ACCESSION   DAZFFG010000479 DAZFFG010000000
VERSION     DAZFFG010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.guided.246"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            <1..402
                     /locus_tag="ACHJ7J_005318"
     CDS             <1..402
                     /locus_tag="ACHJ7J_005318"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5003372.1"
                     /translation="CAIFLRAVTMTASQHYSPQQIAAWAQIDESRWKEKLAKSQVRVA
                     VINAKLVGFITCVEYYIDMLFVDPEYTRRGVASALLKPLIKSESELTVDASITAKPFF
                     ERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            721..927
                     /locus_tag="ACHJ7J_005319"
                     /pseudo
     CDS             721..927
                     /locus_tag="ACHJ7J_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            974..1255
                     /locus_tag="ACHJ7J_005320"
                     /pseudo
     CDS             974..1255
                     /locus_tag="ACHJ7J_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(1312..2769)
                     /gene="pepD"
                     /locus_tag="ACHJ7J_005321"
     CDS             complement(1312..2769)
                     /gene="pepD"
                     /locus_tag="ACHJ7J_005321"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5003373.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3030..>3476
                     /gene="gpt"
                     /locus_tag="ACHJ7J_005322"
     CDS             3030..>3476
                     /gene="gpt"
                     /locus_tag="ACHJ7J_005322"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5003374.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPI"
BASE COUNT          872 a          858 c          869 g          877 t
ORIGIN      
        1 tgcgctattt tccttagagc ggttacgatg accgccagtc agcattattc accacaacaa
       61 attgccgcct gggcgcagat tgacgaatct cgctggaagg agaaactcgc gaaatcacaa
      121 gtgcgggttg cagtcattaa tgcaaaactg gtcggcttta ttacctgcgt tgaatactat
      181 atcgatatgt tatttgttga ccctgaatac acccgccgtg gggttgccag cgctttgtta
      241 aaacctttga ttaagtctga atccgaactt acggtggacg caagcataac cgcaaaaccc
      301 ttttttgaac gttatggttt tcagacagtt aagcagcagc gcgttgaatg ccggggagcg
      361 tggtttacta atttttatat gcgatataaa ccgcaacatt aaatccagct tgcaatgaaa
      421 ataacgcccg cctggtatgt gcgcgttttc tgattccaca aactgcaagg aggtaaatca
      481 tgacaaatcc tttatcaatg actctttgca gacctttcca ggattaattc tttttttctt
      541 gccctggatt cgtctgccat ttcctgattt ttaatattta tatactctaa ataattcgag
      601 ttgcaggaag gtgacaagcg agtgaatcgc caggagctta ctaaagtaag tgactggggt
      661 gaacgaacgc agtcgcagta catgcaacct gaagtatgac gagtataaat ggaatggtac
      721 atgggcaaat atattcgtcc cttatccgat gcggtattta ccatcgcatc tgatgacctg
      781 tggatcgaga gtttagcgat ccaacaatta cacaccacgg caaatttacc caacatgcag
      841 cgcgtagtgg ggatgccaga tttacacccc ggacgcggct acccgattgg cgcagcgttc
      901 ttctctgttg gtcgttttta cccggcaaga cgtcgcggta acggtgctgg aaaccgaagc
      961 cgggtgttat cctgacacgc tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac
     1021 cgactcggcg gtacgcgcta cgcatctggc atccggtatt agcgtgaagg ttcagtcaga
     1081 acgcagccag catgctaaca aacggctggc gcgattgctg attgcctgga agctggagca
     1141 gcagcaacag gaaaatagcg cggcgctgaa atcacagcgg cgaatgttcc atcaccagat
     1201 tgaacgtggc aacccgcgac gaacgtttac agggatggct tttatcgaag gataatgaag
     1261 gataaaactg ccggaaggcg attaaacgcc atccggcaga aaatcaaata attacttcgc
     1321 cggaatttct ttcagcagtt cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg
     1381 aacttgctca tccggagagt gtggaccggt gatagttggc ccgatagaaa ccatgtccat
     1441 ttccggatac ggttttttga acagaccaca ttccaggccc gcgtggataa tctggatgtt
     1501 cggcgtcttg ttgaacaggc gctgataggt ttcacgtacc agatgcatca ccggagaatt
     1561 agcgtccggc tgccagccag gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt
     1621 acccagcgaa tccagcatgc tcaccacgta gtctttaccg ctgtcgatca gagaacggat
     1681 cagacagtga atttctacgt tattgtcagt catggtcacc acaccgacgt tcagggaggt
     1741 ttcgaccaca ccttttgcca cgtcggagtt gcggatcaca ccgttcgggg tggcgttcag
     1801 cagacgaata aaggtatcgc gagatttcgc aatcagggca gctttatcgt tcgctacaga
     1861 gtccagcaac aaggccagat ttttctcttt ctctgccagc tcgtttttca ggatctcctg
     1921 ataggtattc accagagatt tcagggcgtc gactttatca gctgcgacag caatggtcgc
     1981 aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg ttgaaatcaa taagacgcag
     2041 gtccagttct tctgcatgac ccgccaggaa gcgcaccagc agtttgttgg cattacccag
     2101 gccaacgtgg atttccccgc cggagtgacc gcctttcaga ccttttaagg ttaacttgaa
     2161 ggtttcaaaa ccagctggaa ccgcttcacg atctaaatgc aggttggagg tgaagtcgat
     2221 accccccgcg caacccatgt agatttcacc ttcttcttcg gagtcggtgt taatcagaat
     2281 atcagcctgc aaccagttgc tctgtaagcc aaaagcaccg tccataccgg cttcttcggt
     2341 catggtcagc agcacttcca gcggaccgtg aaccacgttt tcgtcagcca gaaccgccag
     2401 tgcagaggcc ataccaatgc cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca
     2461 ttcgccatca atataaggct ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt
     2521 tttctgcggc accatatcga ggtgggcctg caagacgacc ggtttacgat tttccatacc
     2581 tgcggtagca ggtttacgaa tcaggatatt acctacctga tcgcgttcga catggaaacc
     2641 tttctctttt gcccaaccaa caatgtattc agcgagttgc tcttcatgat aggacgggtg
     2701 aggaatagaa cagattttgg caaaaatatc ccacagcggc tgtggagata attgagacag
     2761 ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac agtattgcag gtcacagggt
     2821 tagcagaaaa tgttgtcaac acaagacagg cttgcgagat atgtttgaga ataccacttt
     2881 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     2941 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     3001 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     3061 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     3121 gcattattgc cgtaagccgt ggcggtctgg taccgggtgc gttactggcg cgtgaactgg
     3181 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     3241 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     3301 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     3361 ccatctttgc aaaaccggct ggtcgtccgc tggttgatga ttatgttgtt gatatcccgc
     3421 aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatc
//
LOCUS       DAZFFG010000480         1491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2203M45224
            SAMN43267849-rid23065853.guided.298, whole genome shotgun sequence.
ACCESSION   DAZFFG010000480 DAZFFG010000000
VERSION     DAZFFG010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267849
            Sequence Read Archive: SRR30307047
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350366.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350366.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:11:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,244
            Genes (coding)                    :: 5,089
            CDSs (with protein)               :: 5,089
            Genes (RNA)                       :: 80
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 100 of 155
            Pseudo Genes (internal stop)      :: 40 of 155
            Pseudo Genes (multiple problems)  :: 35 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267849-rid23065853.guided.298"
                     /serotype="O154:H31"
                     /isolate="2203M45224"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-03"
                     /collected_by="SSI"
                     /note="subtype: stx1d"
     gene            61..1008
                     /gene="stxA1d"
                     /locus_tag="ACHJ7J_005323"
     CDS             61..1008
                     /gene="stxA1d"
                     /locus_tag="ACHJ7J_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1d subunit A"
                     /protein_id="HFO5003375.1"
                     /translation="MKIMIFRALTFFFVIFSVNAIAKEFTLDFSTAKKYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDIMGLEPEEERFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTRAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSYSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSILPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMTP
                     DEFPSMCPTDGSGRGITHNKILWDSSTLGAILIRRTISS"
     gene            1018..1287
                     /gene="stxB1d"
                     /locus_tag="ACHJ7J_005324"
     CDS             1018..1287
                     /gene="stxB1d"
                     /locus_tag="ACHJ7J_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1d subunit B"
                     /protein_id="HFO5003376.1"
                     /translation="MKKVLLIAVSLSFLSASVLAAPDCVTGKVEYTKYNDDDTFTVKV
                     ADKELFTNRWNLQSLLLSAQITGMTVTIKTTACHNGGGFSEVIFR"
BASE COUNT          418 a          248 c          359 g          466 t
ORIGIN      
        1 atgtagcagc atttattatg aatatagtta tcgtatggtg ctcaaggagt attgtgtaat
       61 atgaaaataa tgatttttag ggcgctaaca tttttcttcg ttatcttttc agttaatgcg
      121 attgctaagg agtttacctt agatttctca acagcaaaga agtatgttga ttcgctgaat
      181 gtcattcgct ctgcaatagg tacgccatta cagactattt catcgggagg tacatcttta
      241 ctgatgattg atagtggcac aggcgataat ttatttgcag tcgatatcat ggggttagaa
      301 ccagaggaag agcggtttaa caatctacga ctgattgttg aacgaaataa tttatatgtg
      361 acaggatttg ttaacaggac aaacaatgtt ttttatcgct ttgctgattt ttcacacgtt
      421 acctttcctg gtacaagagc ggttacattg tctggtgaca gtagctatac cacgttacag
      481 cgtgttgcgg ggatcagtcg tacggggatg cagataaatc gccattcgct gactacttct
      541 tatctggatt tgatgtcgta tagcggaacc tcactgacgc agtctgtagc aagagcaatg
      601 ttacggtttg ttactgtgac agctgaagct ttgcgttttc gccaaatcca gaggggattt
      661 cgtacaacac ttgatgatct cagtggacgt tcttatgtaa tgactgctga agatgttgat
      721 cttacattga attggggaag actgagtagt attctgcccg actatcatgg acaagactcc
      781 gttcgtgttg gaagaatttc ttttgggagt attaatgcaa ttctgggaag cgtggcatta
      841 atattaaatt gccatcatca tgcatcacga gttgccagaa tgacacctga tgagtttcct
      901 tctatgtgcc cgacagatgg aagtgggcgt gggattactc acaataaaat attgtgggac
      961 tcatctacac tgggggcaat tttgattcgc agaactatca gtagttgagg gggtaaaatg
     1021 aaaaaagtat tattaatagc tgtttcactt tcatttcttt cagcaagtgt tctggcagcg
     1081 ccagattgtg taaccggaaa ggtggagtat acaaaatata atgatgacga tacttttaca
     1141 gttaaagtgg cagataaaga attatttact aacagatgga atcttcagtc tcttcttcta
     1201 agtgcacaaa ttacggggat gacggtaact attaagacta ctgcttgtca taatggaggg
     1261 ggattcagcg aggttatttt tcgttgattc agaatagccc tgtgaagagc acaggaggag
     1321 gtttatcaat gttaaatatg tcaattcagt cagttgacag ttgcctgact ggcagagctt
     1381 ttgtaaaaaa atcttgcata gggaatcccg cagtgcagag tggaaactgg tggacgatat
     1441 catctctgat catcgttaac gataatgagg cgggttcagt ggtgcagggc t
//
